LOCUS       DAZGCS010000001        96574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGCS010000001 DAZGCS010000000
VERSION     DAZGCS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.001"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..565)
                     /gene="ppiA"
                     /locus_tag="ACHK01_000001"
                     /gene_synonym="rotA"
     CDS             complement(<1..565)
                     /gene="ppiA"
                     /locus_tag="ACHK01_000001"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO8095019.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKV"
     gene            836..2017
                     /gene="tsgA"
                     /locus_tag="ACHK01_000002"
     CDS             836..2017
                     /gene="tsgA"
                     /locus_tag="ACHK01_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO8095020.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            2279..4822
                     /gene="nirB"
                     /locus_tag="ACHK01_000003"
     CDS             2279..4822
                     /gene="nirB"
                     /locus_tag="ACHK01_000003"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO8095021.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTEDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4819..5145
                     /gene="nirD"
                     /locus_tag="ACHK01_000004"
     CDS             4819..5145
                     /gene="nirD"
                     /locus_tag="ACHK01_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO8095022.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            5272..6078
                     /gene="nirC"
                     /locus_tag="ACHK01_000005"
     CDS             5272..6078
                     /gene="nirC"
                     /locus_tag="ACHK01_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO8095023.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            6097..7470
                     /gene="cysG"
                     /locus_tag="ACHK01_000006"
     CDS             6097..7470
                     /gene="cysG"
                     /locus_tag="ACHK01_000006"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO8095024.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     MNWFSNH"
     gene            7717..7884
                     /gene="yhfL"
                     /locus_tag="ACHK01_000007"
     CDS             7717..7884
                     /gene="yhfL"
                     /locus_tag="ACHK01_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO8095025.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            8179..9516
                     /gene="frlA"
                     /locus_tag="ACHK01_000008"
     CDS             8179..9516
                     /gene="frlA"
                     /locus_tag="ACHK01_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO8095026.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGAQGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            9537..10559
                     /gene="frlB"
                     /locus_tag="ACHK01_000009"
     CDS             9537..10559
                     /gene="frlB"
                     /locus_tag="ACHK01_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO8095027.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQHGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            10609..11439
                     /gene="frlC"
                     /locus_tag="ACHK01_000010"
     CDS             10609..11439
                     /gene="frlC"
                     /locus_tag="ACHK01_000010"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO8095028.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            11436..12221
                     /gene="frlD"
                     /locus_tag="ACHK01_000011"
     CDS             11436..12221
                     /gene="frlD"
                     /locus_tag="ACHK01_000011"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO8095029.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGPI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            12321..13052
                     /gene="frlR"
                     /locus_tag="ACHK01_000012"
                     /gene_synonym="yhfR"
     CDS             12321..13052
                     /gene="frlR"
                     /locus_tag="ACHK01_000012"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO8095030.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(13204..14289)
                     /gene="yhfS"
                     /locus_tag="ACHK01_000013"
     CDS             complement(13204..14289)
                     /gene="yhfS"
                     /locus_tag="ACHK01_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8095031.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANISTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(14301..15605)
                     /gene="yhfT"
                     /locus_tag="ACHK01_000014"
     CDS             complement(14301..15605)
                     /gene="yhfT"
                     /locus_tag="ACHK01_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO8095032.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVKQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(15617..15970)
                     /gene="yhfU"
                     /locus_tag="ACHK01_000015"
     CDS             complement(15617..15970)
                     /gene="yhfU"
                     /locus_tag="ACHK01_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO8095033.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(15981..16859)
                     /gene="php"
                     /locus_tag="ACHK01_000016"
     CDS             complement(15981..16859)
                     /gene="php"
                     /locus_tag="ACHK01_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO8095034.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(16856..18082)
                     /gene="yhfW"
                     /locus_tag="ACHK01_000017"
     CDS             complement(16856..18082)
                     /gene="yhfW"
                     /locus_tag="ACHK01_000017"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8095035.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(18082..19245)
                     /gene="yhfX"
                     /locus_tag="ACHK01_000018"
     CDS             complement(18082..19245)
                     /gene="yhfX"
                     /locus_tag="ACHK01_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO8095036.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIVIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(19329..19691)
                     /gene="yhfY"
                     /locus_tag="ACHK01_000019"
     CDS             complement(19329..19691)
                     /gene="yhfY"
                     /locus_tag="ACHK01_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO8095037.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(19708..20613)
                     /gene="yhfZ"
                     /locus_tag="ACHK01_000020"
     CDS             complement(19708..20613)
                     /gene="yhfZ"
                     /locus_tag="ACHK01_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO8095038.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(20882..21886)
                     /gene="trpS"
                     /locus_tag="ACHK01_000021"
     CDS             complement(20882..21886)
                     /gene="trpS"
                     /locus_tag="ACHK01_000021"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO8095039.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(21879..22637)
                     /gene="gph"
                     /locus_tag="ACHK01_000022"
     CDS             complement(21879..22637)
                     /gene="gph"
                     /locus_tag="ACHK01_000022"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO8095040.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(22630..23307)
                     /gene="rpe"
                     /locus_tag="ACHK01_000023"
     CDS             complement(22630..23307)
                     /gene="rpe"
                     /locus_tag="ACHK01_000023"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO8095041.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(23325..24161)
                     /gene="dam"
                     /locus_tag="ACHK01_000024"
     CDS             complement(23325..24161)
                     /gene="dam"
                     /locus_tag="ACHK01_000024"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8095042.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(24268..25554)
                     /gene="damX"
                     /locus_tag="ACHK01_000025"
     CDS             complement(24268..25554)
                     /gene="damX"
                     /locus_tag="ACHK01_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO8095043.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(25646..26734)
                     /gene="aroB"
                     /locus_tag="ACHK01_000026"
     CDS             complement(25646..26734)
                     /gene="aroB"
                     /locus_tag="ACHK01_000026"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO8095044.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(26791..27312)
                     /gene="aroK"
                     /locus_tag="ACHK01_000027"
     CDS             complement(26791..27312)
                     /gene="aroK"
                     /locus_tag="ACHK01_000027"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO8095045.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(27713..28951)
                     /gene="hofQ"
                     /locus_tag="ACHK01_000028"
     CDS             complement(27713..28951)
                     /gene="hofQ"
                     /locus_tag="ACHK01_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO8095046.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(28863..29267)
                     /gene="hofP"
                     /locus_tag="ACHK01_000029"
     CDS             complement(28863..29267)
                     /gene="hofP"
                     /locus_tag="ACHK01_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO8095047.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(29257..29697)
                     /gene="hofO"
                     /locus_tag="ACHK01_000030"
     CDS             complement(29257..29697)
                     /gene="hofO"
                     /locus_tag="ACHK01_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO8095048.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(29681..30220)
                     /gene="hofN"
                     /locus_tag="ACHK01_000031"
     CDS             complement(29681..30220)
                     /gene="hofN"
                     /locus_tag="ACHK01_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO8095049.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(30220..30999)
                     /gene="hofM"
                     /locus_tag="ACHK01_000032"
     CDS             complement(30220..30999)
                     /gene="hofM"
                     /locus_tag="ACHK01_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO8095050.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            31119..33671
                     /gene="mrcA"
                     /locus_tag="ACHK01_000033"
     CDS             31119..33671
                     /gene="mrcA"
                     /locus_tag="ACHK01_000033"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO8095051.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(33836..34396)
                     /gene="nudE"
                     /locus_tag="ACHK01_000034"
     CDS             complement(33836..34396)
                     /gene="nudE"
                     /locus_tag="ACHK01_000034"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO8095052.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            34716..36851
                     /gene="igaA"
                     /locus_tag="ACHK01_000035"
     CDS             34716..36851
                     /gene="igaA"
                     /locus_tag="ACHK01_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO8095053.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            36916..37584
                     /gene="yrfG"
                     /locus_tag="ACHK01_000036"
     CDS             36916..37584
                     /gene="yrfG"
                     /locus_tag="ACHK01_000036"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO8095054.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            37595..37996
                     /gene="hslR"
                     /locus_tag="ACHK01_000037"
     CDS             37595..37996
                     /gene="hslR"
                     /locus_tag="ACHK01_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO8095055.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            38021..38899
                     /gene="hslO"
                     /locus_tag="ACHK01_000038"
     CDS             38021..38899
                     /gene="hslO"
                     /locus_tag="ACHK01_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO8095056.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(39034..40758)
                     /gene="yhgE"
                     /locus_tag="ACHK01_000039"
     CDS             complement(39034..40758)
                     /gene="yhgE"
                     /locus_tag="ACHK01_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO8095057.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            41136..42758
                     /gene="pckA"
                     /locus_tag="ACHK01_000040"
     CDS             41136..42758
                     /gene="pckA"
                     /locus_tag="ACHK01_000040"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO8095058.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(42834..44186)
                     /gene="envZ"
                     /locus_tag="ACHK01_000041"
     CDS             complement(42834..44186)
                     /gene="envZ"
                     /locus_tag="ACHK01_000041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO8095059.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(44183..44902)
                     /gene="ompR"
                     /locus_tag="ACHK01_000042"
     CDS             complement(44183..44902)
                     /gene="ompR"
                     /locus_tag="ACHK01_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO8095060.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            45130..45606
                     /gene="greB"
                     /locus_tag="ACHK01_000043"
     CDS             45130..45606
                     /gene="greB"
                     /locus_tag="ACHK01_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO8095061.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            45703..48024
                     /gene="yhgF"
                     /locus_tag="ACHK01_000044"
     CDS             45703..48024
                     /gene="yhgF"
                     /locus_tag="ACHK01_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO8095062.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            48462..48689
                     /gene="feoA"
                     /locus_tag="ACHK01_000045"
     CDS             48462..48689
                     /gene="feoA"
                     /locus_tag="ACHK01_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO8095063.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            48706..51027
                     /gene="feoB"
                     /locus_tag="ACHK01_000046"
     CDS             48706..51027
                     /gene="feoB"
                     /locus_tag="ACHK01_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO8095064.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            51027..51263
                     /gene="feoC"
                     /locus_tag="ACHK01_000047"
     CDS             51027..51263
                     /gene="feoC"
                     /locus_tag="ACHK01_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO8095065.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            51466..52344
                     /gene="rpnA"
                     /locus_tag="ACHK01_000048"
     CDS             51466..52344
                     /gene="rpnA"
                     /locus_tag="ACHK01_000048"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO8095066.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(52373..53143)
                     /gene="bioH"
                     /locus_tag="ACHK01_000049"
     CDS             complement(52373..53143)
                     /gene="bioH"
                     /locus_tag="ACHK01_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO8095067.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            53181..53864
                     /gene="gntX"
                     /locus_tag="ACHK01_000050"
     CDS             53181..53864
                     /gene="gntX"
                     /locus_tag="ACHK01_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO8095068.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            53923..54498
                     /gene="nfuA"
                     /locus_tag="ACHK01_000051"
     CDS             53923..54498
                     /gene="nfuA"
                     /locus_tag="ACHK01_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO8095069.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            54859..56175
                     /gene="gntT"
                     /locus_tag="ACHK01_000052"
     CDS             54859..56175
                     /gene="gntT"
                     /locus_tag="ACHK01_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8095070.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(56220..58304)
                     /gene="malQ"
                     /locus_tag="ACHK01_000053"
     CDS             complement(56220..58304)
                     /gene="malQ"
                     /locus_tag="ACHK01_000053"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO8095071.1"
                     /translation="MESKRLDNAALAAGISPNYINAYGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDHE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(58314..60707)
                     /gene="malP"
                     /locus_tag="ACHK01_000054"
     CDS             complement(58314..60707)
                     /gene="malP"
                     /locus_tag="ACHK01_000054"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO8095072.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            61319..64024
                     /gene="malT"
                     /locus_tag="ACHK01_000055"
     CDS             61319..64024
                     /gene="malT"
                     /locus_tag="ACHK01_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO8095073.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(64067..65083)
                     /gene="rtcA"
                     /locus_tag="ACHK01_000056"
     CDS             complement(64067..65083)
                     /gene="rtcA"
                     /locus_tag="ACHK01_000056"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO8095074.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGIVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(65087..66313)
                     /gene="rtcB"
                     /locus_tag="ACHK01_000057"
     CDS             complement(65087..66313)
                     /gene="rtcB"
                     /locus_tag="ACHK01_000057"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO8095075.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            66502..68100
                     /gene="rtcR"
                     /locus_tag="ACHK01_000058"
     CDS             66502..68100
                     /gene="rtcR"
                     /locus_tag="ACHK01_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO8095076.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSVFQLISGTVRDL
                     RQRVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(68082..68840)
                     /locus_tag="ACHK01_000059"
     CDS             complement(68082..68840)
                     /locus_tag="ACHK01_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO8095077.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(68857..69687)
                     /gene="glpG"
                     /locus_tag="ACHK01_000060"
     CDS             complement(68857..69687)
                     /gene="glpG"
                     /locus_tag="ACHK01_000060"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO8095078.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(69732..70058)
                     /gene="glpE"
                     /locus_tag="ACHK01_000061"
     CDS             complement(69732..70058)
                     /gene="glpE"
                     /locus_tag="ACHK01_000061"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO8095079.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            70248..71753
                     /gene="glpD"
                     /locus_tag="ACHK01_000062"
     CDS             70248..71753
                     /gene="glpD"
                     /locus_tag="ACHK01_000062"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO8095080.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(71805..72410)
                     /locus_tag="ACHK01_000063"
     CDS             complement(71805..72410)
                     /locus_tag="ACHK01_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095081.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(72413..73180)
                     /locus_tag="ACHK01_000064"
     CDS             complement(72413..73180)
                     /locus_tag="ACHK01_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095082.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(73183..73893)
                     /locus_tag="ACHK01_000065"
     CDS             complement(73183..73893)
                     /locus_tag="ACHK01_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095083.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(73909..75417)
                     /locus_tag="ACHK01_000066"
     CDS             complement(73909..75417)
                     /locus_tag="ACHK01_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8095084.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(75542..77989)
                     /gene="glgP"
                     /locus_tag="ACHK01_000067"
     CDS             complement(75542..77989)
                     /gene="glgP"
                     /locus_tag="ACHK01_000067"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO8095085.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(78008..79441)
                     /gene="glgA"
                     /locus_tag="ACHK01_000068"
     CDS             complement(78008..79441)
                     /gene="glgA"
                     /locus_tag="ACHK01_000068"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO8095086.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(79441..80736)
                     /gene="glgC"
                     /locus_tag="ACHK01_000069"
     CDS             complement(79441..80736)
                     /gene="glgC"
                     /locus_tag="ACHK01_000069"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO8095087.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(80754..82727)
                     /gene="glgX"
                     /locus_tag="ACHK01_000070"
     CDS             complement(80754..82727)
                     /gene="glgX"
                     /locus_tag="ACHK01_000070"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO8095088.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(82724..84910)
                     /gene="glgB"
                     /locus_tag="ACHK01_000071"
     CDS             complement(82724..84910)
                     /gene="glgB"
                     /locus_tag="ACHK01_000071"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO8095089.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(85183..86286)
                     /gene="asd"
                     /locus_tag="ACHK01_000072"
     CDS             complement(85183..86286)
                     /gene="asd"
                     /locus_tag="ACHK01_000072"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8095090.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            86479..87072
                     /gene="yhgN"
                     /locus_tag="ACHK01_000073"
     CDS             86479..87072
                     /gene="yhgN"
                     /locus_tag="ACHK01_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO8095091.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(87118..88413)
                     /locus_tag="ACHK01_000074"
     CDS             complement(87118..88413)
                     /locus_tag="ACHK01_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO8095092.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHENDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(88320..90416)
                     /locus_tag="ACHK01_000075"
     CDS             complement(88320..90416)
                     /locus_tag="ACHK01_000075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO8095093.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(90556..91896)
                     /gene="gntU"
                     /locus_tag="ACHK01_000076"
     CDS             complement(90556..91896)
                     /gene="gntU"
                     /locus_tag="ACHK01_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8095094.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPIIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(91900..92427)
                     /gene="gntK"
                     /locus_tag="ACHK01_000077"
     CDS             complement(91900..92427)
                     /gene="gntK"
                     /locus_tag="ACHK01_000077"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO8095095.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(92566..93561)
                     /gene="gntR"
                     /locus_tag="ACHK01_000078"
     CDS             complement(92566..93561)
                     /gene="gntR"
                     /locus_tag="ACHK01_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO8095096.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(93785..94480)
                     /gene="yhhW"
                     /locus_tag="ACHK01_000079"
     CDS             complement(93785..94480)
                     /gene="yhhW"
                     /locus_tag="ACHK01_000079"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO8095097.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(94603..95640)
                     /gene="yhhX"
                     /locus_tag="ACHK01_000080"
     CDS             complement(94603..95640)
                     /gene="yhhX"
                     /locus_tag="ACHK01_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8095098.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            95972..96460
                     /gene="yhhY"
                     /locus_tag="ACHK01_000081"
     CDS             95972..96460
                     /gene="yhhY"
                     /locus_tag="ACHK01_000081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8095099.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        23267 a        26195 c        24296 g        22816 t
ORIGIN      
        1 ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac ggaccaacgt
       61 catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc actttaccaa
      121 ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg gcaacgttga
      181 taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg atggtgccac
      241 gcgtgttgcg caggccgtta tcggcttcat ttttgattgg cgggtttggt tttttctgct
      301 gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg cggtgaaagg
      361 tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca gacactggcg
      421 ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac aatacgtgcg
      481 ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcgaaa acagcagcca
      541 tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag aaaaaaagca
      601 agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta attttacgta
      661 tctgaaatat atgtaacctg acgatcactt tttattccgt gatcaaaatc acctcttaaa
      721 atgcaattta gcaaccgatt gcaataaaac atttaaacag atcacaaaat cacctaaaat
      781 cgcccgctcg cggcatatcc cgctatgcca ttttattttt gaacgcaggc caatcatgac
      841 taacagcaat cgcatcaagc tcacatggat tagctttctc tcctacgcac tgactggtgc
      901 gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca atctgcctgt
      961 ttccagtatg agtaacacct tcaccttcct caacgccggc attttaatct ctatcttcct
     1021 caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg gctttctcct
     1081 gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt tctcggcggc
     1141 gatgttcatt ctcggggtgg tcagcggcat caccatgtcg attggtacat tcctggtaac
     1201 acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta tttaccgact ccttcttcag
     1261 tatggctggg atgattttcc caatgatcgc cgcgtttcta ctggcgcgca gcattgagtg
     1321 gtactgggtt tatgcctgca tcgggctggt gtatgtcgct atttttattc tgaccttcgg
     1381 ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg gatgctccgg tagagaaaga
     1441 aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca tcctcggtca
     1501 gttaggtttt atctcctggg tgcctgagta tgccaaaggc ctgggcatga gcctgaacga
     1561 cgcgggcacg ctggtgagta acttctggat gtcatacatg gtcggcatgt gggcgttcag
     1621 ctttattctt cgcttctttg atttgcaacg cattctgacc gtactggctg gtctggctgc
     1681 gattctgatg tacgtcttta acaccggaac accagcacat atggcgtggt caattctcgc
     1741 tctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt cacagcagac
     1801 caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcggaacca tcggtactat
     1861 gttgaccttt gtggttaccg gcccgattgt tgaacatagc ggtccgcagg cggcactgct
     1921 tacagcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag gtttcgtcag
     1981 ccgtcaccgt cagcataaca ccctgacctc tcattaattg ctaatgccgg acggcactat
     2041 cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat ctatttctat aaacccgttc
     2101 attttgtcta ttttttgcac aaacatgaaa tatcagacaa ttccgtgact taagaaaatt
     2161 tatacaaatc agcaatatac cccttaagga gtatataaag gtgaatttga tttacatcaa
     2221 taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg aggcaaaaat
     2281 gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct ttatcgaaga
     2341 tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg aagaaccgcg
     2401 catcgcttat gaccgcgtac acctctcgtc ttacttctct caccacaccg ccgaagagct
     2461 gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg tcggcgaacg
     2521 cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccgggc gtaccgtttt
     2581 ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg atcccgccaa tcaaaggttc
     2641 tgatactgag gactgctttg tctatcgcac cattgaagac ctcaacgcca ttgaatcctg
     2701 cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc ctgttaggtc tggaagccgc
     2761 tggcgcgctg aaaaacttag gtattgaaac ccacgttatc gaatttgccc ctatgctgat
     2821 ggcggaacag cttgatcaga tgggtggcga gcagctgcgt cgcaaaatcg aaagtatggg
     2881 cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg caggaaggtg tcgaagcgcg
     2941 taaaaccatg cgttttgccg acggcagcga gctggaagtt gactttatcg tcttctctac
     3001 cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg ctccgcgtgg
     3061 gggtattgtc attaatgatt cctgccagac ttccgatccg gatatctacg ccatcggtga
     3121 atgcgcaagc tggaacaacc gtgtatttgg tctggtagca ccaggctaca aaatggcgca
     3181 ggtcgccgtt gaccatattc tgggtagcga aaacgccttt gaaggtgctg accttagcgc
     3241 caagctgaaa ctgctgggcg tagacgtagg cggtattggt gatgcgcacg gtcgcacgcc
     3301 tggcgcacgt agctacgttt acctcgacga aagtaaagag atctacaaac gcctgattgt
     3361 cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca gcgactacgg
     3421 taacctgctg caactggtgc tgaacgctat cgaactgccg gaaaacccgg attccctgat
     3481 cctgccagca cactctggta gcggcaagcc gtctatcggt gttgataaac tgccggacag
     3541 cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat ctgattgctg ccatcaacaa
     3601 aggctgccac accgttgcgg cgctgaaagc tgaaaccaaa gcgggtactg gctgcggtgg
     3661 ctgtatcccg ctggttactc aggtactgaa cgcggaactg gcgaaacagg gcatcgaagt
     3721 taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactgttcc atttgatccg
     3781 cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag gctacggttg
     3841 tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc tgctggaacg aatacattct
     3901 gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcta acatccagaa
     3961 agacggcacc tactcggtga tcccgcgttc tccgggcggt gaaatcaccc cggaagggct
     4021 gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac accaaaatca ccggctccca
     4081 gcgcctggcg atgtttggcg cacagaaaga cgatctgccg gaaatctggc gtcagctgat
     4141 tgaagctggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg cgaaaacctg
     4201 cgtgggtagc acctggtgcc gctacggtgt tggcgatagc gtcggcctcg gcgtggaact
     4261 ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg tctccggctg
     4321 tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca ctgaaaaagg
     4381 ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg atctgctggc
     4441 agcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga tgttctacat
     4501 ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg aaggcggcat
     4561 cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgctc atctggaaga
     4621 agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg actgaaacgg tcaatacacc
     4681 gtccgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc cgaacgtgca
     4741 gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta tcccggtaac
     4801 tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga tgacatcctg
     4861 cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg tagcaatttt ccgcccgtat
     4921 cacagcgatc aggtgtttgc gatcagcaac atcgacccgt tcttcgagtc cagcgtgctg
     4981 tcacgcgggc tgattgcgga acaccagggc gagctgtggg tcgccagccc gctgaaaaaa
     5041 cagcgttttc gcttaagtga tggcttgtgc atggaagacg aacagttttc cgtcaaacat
     5101 tacgaagcac gagtgaaaga cggcgtggtg cagcttcgcg gttaatgttt taacgggagg
     5161 cgcaatgcct cccctttttg catggtcctg taatgacctt cggtatattg caggacattt
     5221 tttaaacttt ttgttttatt tttttgtttt tattttttaa aggataatca aatgtttaca
     5281 gacactatta ataagtgtgc ggctaacgct gcgcgcattg cacgcctgtc ggcaaataac
     5341 ccgctcggct tttgggtcag ctccgccatg gcgggcgcgt atgtgggtct tgggatcatc
     5401 ttgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt gatgggcgcg
     5461 acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt caccggacac
     5521 accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca aatgtgggca
     5581 atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctctat
     5641 agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc cgtcgcgctg
     5701 gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg
     5761 gtttgcctgg caatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc
     5821 tggtggtgtc tgctggcatt tatcgcgtct ggctacgagc actctatcgc taacatgacg
     5881 ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc gggtattggt
     5941 cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt catgggattg
     6001 ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact
     6061 gaaacggctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc
     6121 caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca
     6181 aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag
     6241 ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc
     6301 cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacgcgct taaccagcgc
     6361 gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tggtcgatgc gccgaaagcc
     6421 gccagcttta ttatgccgtc gattattgac cgctcaccgc tcatggtagc ggtctcctct
     6481 ggcggcacct ctccggttct ggcacgcctg ttgcgcgaaa aacttgaatc actgctgccg
     6541 ttgcatctgg gccaggtagc gaaatacgcc gggcaattac gcgggcgagt gaaacaacag
     6601 ttcgccacga tgggtgagcg tcgccgtttc tgggagaaat tgttcgttaa cgatcgcctg
     6661 gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaaccaccga acagttaatc
     6721 aacgaaccgc ttgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc
     6781 gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt ggtctacgac
     6841 cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc
     6901 ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat cctgctgcgg
     6961 gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt tatttttggc
     7021 cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc ggtggttccg
     7081 ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac gcatcgcgat
     7141 tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga gctggactgg
     7201 gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa tcaggccgcg
     7261 actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt ggcaattgtc
     7321 gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca actcggtgaa
     7381 cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc
     7441 gataaaatga actggttctc caaccattaa tttaacccgg gccagagaat tctggccttc
     7501 ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa atattatctg
     7561 ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg ctcactgacg
     7621 ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca tataatcaat
     7681 gaatattaat taatatataa tacataggga atgtaaatga acaaatttat taaagttgca
     7741 ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat
     7801 aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc
     7861 ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg
     7921 cccgacgccc tctggatttt ccgagggcgt atttgtttct atatccttct tgcccccgcc
     7981 cgtaaaaata ttttcacttt aaattcaatt tgataactac atccattagc cacagcgcga
     8041 tcctgtgcga aattttgtga tcttcctcca cattacataa catcatatgt tgttatattc
     8101 atcatgcatt gtcatgttac cttttaaatg actgcaaact ctcccctaca acgtattgga
     8161 caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg attttgggcc
     8221 gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa
     8281 gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat tggcgggtta
     8341 attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc
     8401 gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg
     8461 gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc
     8521 aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta
     8581 attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg
     8641 ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg
     8701 tttaaagcgg agaattttgc cgcccctacc accactgcga ttggcgcaac gggcagcttt
     8761 atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc
     8821 tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt
     8881 tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg
     8941 cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctggatcccc
     9001 gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt cattcttggt
     9061 tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac
     9121 ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc
     9181 atcctgcaag gggcgcaggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg
     9241 ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg
     9301 tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc
     9361 accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt
     9421 cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg
     9481 gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctggagaa aataaaatgt
     9541 tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg
     9601 aaaaagttct cagccatgac gttccgctgg tgcacaccat cgtggaagag atggtgaagc
     9661 gcgacattga tcgtatttat ttcgttgcct gcggatcgcc actcaacgcg gcgcaaacgg
     9721 cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt
     9781 tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact
     9841 acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca
     9901 ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg
     9961 attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg
    10021 agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc
    10081 agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcatg
    10141 gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc
    10201 tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga
    10261 ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc
    10321 tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta
    10381 aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc
    10441 cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta
    10501 tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat
    10561 ccctctcccg gcccggcaac gggccgaatt tacgcaagga gttacccgat gaaaacaggt
    10621 atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag
    10681 ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta
    10741 aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc
    10801 ggctatacgc cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg
    10861 cgtcgcgaaa gcctcgacat gatcaagctg gcgatggata tggcaaaaga gatgaacgcg
    10921 ggttatacgc tgatttccgc ggcccacgcg ggctatctca cgccacctaa tgttatctgg
    10981 ggacggctgg cagagaacct aagcgaactg tgtgagtacg cggaaaacat cggcatggat
    11041 ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg
    11101 cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga catttgcgcg
    11161 ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggtga taaattgcgt
    11221 catctgcata ttgtcgacag cgacggggcc agtgacacac attacattcc cggcgaaggc
    11281 aaaatgccgc tgcgggagct gatgcgcgat attatcgacc ggggctatga cggttactgc
    11341 acggtggaac tggtgacgat gtatatgaac gagcccagac tctatgcccg ccaggcgctg
    11401 gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctggcg acaatcggcg
    11461 ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt aatgcggtca
    11521 atgtggcggt gtactgcact cgctacggca tacagccggg acccattacc tgggtgggtg
    11581 acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc gatatcagcc
    11641 atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac gacaatgatc
    11701 gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt gaagaggatt
    11761 acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat gcggaagacg
    11821 cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg
    11881 acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc
    11941 aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc gcaggaacag
    12001 tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag ttctggcgtc
    12061 aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg ttcattgccg
    12121 gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg cagggaacgg
    12181 cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt tggcgtgagc
    12241 atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg agtaattcat
    12301 tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa ctcctctacg
    12361 ctaccgtccg ccagcgactg ctggatgata tcgcgcaggg ggtttaccag gccgggcaac
    12421 agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt accattcgca
    12481 aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga aaaggcacct
    12541 ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt accgattttg
    12601 gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga acaggaacgg gtcagcgccg
    12661 cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat ctctgccggg
    12721 tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt
    12781 atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta tttcaggagc
    12841 gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc gccacccgcc
    12901 cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg
    12961 cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc gccaatcgca
    13021 tcaccttaac tattgataat aaaagacatt aaccgtaggc cggataagat gcgccagcat
    13081 cgcatccggc gatgctggcg cgttgaattt tacatcccgt acgttcctct caccctaacc
    13141 ctctccccaa aggggcgagg ggaccgttca gtacggaatt tagcttgacg gtgctatcca
    13201 gcattaaata ctggcaatac tctcacgcaa aatccgcagc accgtctctt caccgctgcg
    13261 attcgggtta atgcgaatcg cacaatgttc tgattgtgga ttcgcctggc gaaacgttcc
    13321 ggaaaggcga taaaagagcg gcgggatttc atatttcgac tctgcaccca ccgggtaagg
    13381 caaggcaccg cgcttttgcg cctcttccag cactctggcg gcaatcggct gatgaaactc
    13441 gacaatcaac accttcgact gcgcattagc aatcaccgcg cttttcactt ccggcaccgc
    13501 accaccgtta agcaaagcca gcaaccgttc agatacccct gcctgcaccg cgtgcatcac
    13561 tggcgcaaac accagaccac gcaagacttc cagcgcctgt gcgccctgga tctggctacc
    13621 gccggagtaa agcgtggcgc gaatacggtt gataacatca gcatcgccga ccactgcacc
    13681 aacaccctct ggcccaaata gcttgaagca ggaaaatgtc gagatattcg cgccgcattc
    13741 acagccgatt cgcgccacct tcatcaccgc atagttgtca tcggttaacg ctggaacacc
    13801 tgccgcgcgc aacgttgcca gcacatctgc cagcacgtag ctgtcctgcg gctgctggcg
    13861 cgtatgctgc accagcgccg catccggttg ttgctcgtcg acgacctgct tcagtgccga
    13921 caggtcattg aaatcaacag taataagcgt cagccccatc tgctcaataa taacccgtgt
    13981 cgtcgggtaa acaggcgcgt catgcaccag aagacgctgc cccggtttga gcaaagccgc
    14041 caacccggcg cgaatcgcgc cagtccccgc gccctgcacc agcgccgcag cctgtgcgtg
    14101 aaatgcatca gccagcacct gttccacacg ctgggtaacg cgcggttgat tcagtcctgg
    14161 cgttaagcct aaatcaccgc cagtaagaaa ctcgctgccg gggaaatggc gacaaatgct
    14221 atccaccagc gcaaactgct tttgctgcgc ctcaataatc gtcaggcttt gcagaggaaa
    14281 cgtcttcata ggtgccttcc ttaagccgga acgaacaggc caagccagta aagaacattc
    14341 agcagaatac cggtgatcat tactgccacg accggtgccg ccattttctg taccggacgg
    14401 cccagggatt cgttgaggaa gtaaatggca accgcgatag agaatccggt ataacccgcc
    14461 atcttaattg ctgcgaaaat cgaaccgacc agcagcgcca cttccatcag catattcatg
    14521 gcgttacgga tgttatccga cgcattacgc accgacgggt agcgtcccag ccatttgccg
    14581 atcgaacgaa gcagcaagac ttccgccgaa ataaccactg cgcctaatac cgctgcaacc
    14641 atcggattcg gcgagagata gcccaccgca taaacaaagg taaagcccgc aactgcatac
    14701 acacccgttg ctaaggcggt ggtggcaatc aacggtacaa aacccagccc gcgcataaat
    14761 tctgccagtg ccgcctggtt gatcagcgtt tgcgattgtt ccggcgttac gcctgcggag
    14821 tacgctttct ccagagtgaa gatcgacact tcactgccgg cgaaaatctt catgctggca
    14881 acggcggcaa tcaatgctcc cacgatggcg atatagggta agtttttgat aatccgcgac
    14941 gtgcgttctt caaacaccga aagcccgctg gcatcaatat cattttcatc acgatgacgc
    15001 aggtcgtgag ttatcgcgat ccccagcagc atcaccatgc caataaagat ttcgatggat
    15061 tcagggttaa gatgtgggaa atagcgcacg actaccacac gggtcatcag aacaaccacg
    15121 gcggcgacca gactttgctt ccagccaaac tggtagaaaa tcgccaccag cgggaacagt
    15181 gcaaaagcag aaaccaccgg cgagcttaat tccccgaggc tacctaatac atccaccggc
    15241 agcgcggtca gcagctgctt tactggcaac aggcaagtaa ggatcaatac gccccagata
    15301 gcgccaagac caaacgccat caggctgttt atcgccagta cgccgaggat atcggtagga
    15361 agaaagagta accatgcgtt gagcagtccg gttttcaggg taaaagagat ccccaccgag
    15421 gccacaaaac caatgctcag accaaaagcg atactccccg cctcgcgacg gttcatatag
    15481 ccttcaatca gttgcggcag gatcgggcgg atgccgtcat gaaaaacagc cgccgagcga
    15541 tgcgccagaa gcgatgtcat acccgtcagg cacgccacca cgataatctg aatatacaga
    15601 tccatagtcg tgccttttat ttcagatggt taatcagcat cggaatcgcg tgttcgacgt
    15661 gctcaacgga aaggccaaac gccacttttc cttcagcgat cattttggcg atatgctcgt
    15721 ctttcgcttt aatgcctggt ttggcaatgg tgcaactttt gttatagcca atcaccgcga
    15781 tggcaatcga caatgccgcg cccgcaccgg tattacacgc accgatgtag taatccagtt
    15841 gcccggattt cactttcatt gccgcttcca tgtcgttgtg aatgaaaact tcaaaactgc
    15901 caggagccgt cgcttcaatg gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa
    15961 tctttttcat gagtctgtcc ttattggaaa aattgagagg gagtttcacg taacatcaca
    16021 tccacatcgg cctgactgaa tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa
    16081 tagtcatagc cataaccacc attggctttt aaatgggagc ggcgcgtaat atccatcgac
    16141 agcatgacgc ggttcagcaa cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt
    16201 tcgtccgggt agtaactgtt cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg
    16261 atcatcttca aaatgttgtc gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc
    16321 gaaagatcaa ctccgtgggc ttgcaacaga gccagttgtt ccagccccat ggtgctgaac
    16381 gaagtatgcg tggagatcgg gcgtccggtc tggttatgcg ccagcgcagc ggcaataaac
    16441 accttctctt ccagcggcgt aatcttccct tcgctagagc cgatctccgc gataatcccg
    16501 gctttcaggt cggtgccatc gataccctgt tcaatttcat cgaccatctc ctgcgccagt
    16561 tcctgcacgc tgcgggtcgc cacatgctcc gggaaaaaag cgtcctggta ataaccggtg
    16621 caggccacca cgttgatccc cgtctcgcgc attacatcga gcataaattg cgcattgcgc
    16681 cccatgtaac ggttggtcat ctcaatcaca ttacgcacgc cccgggtcat caggtcgttc
    16741 atctcctggc aaatgaacgc atactgatca aggcggcagt ccacgttgtt tttaaagccg
    16801 gagagatcaa tatgcagatg ctcatgggcc agggtgtaac ccgtcggatc aaaactcata
    16861 gggtgtctcc tgcaaaccgg agggaggaaa gaaaagagcg accattttgt ggcggtggcg
    16921 cacggaaaaa ttcacacacg gtagccccca catctgaaag cgtggtgcgc acaccgagct
    16981 gcgtagctat catcccttgc tgataaacca gcactggcac cacttcgcgg gtatggtggc
    17041 tgtgaccaat ggtcggatcg ttaccgtgat ccgccatcac gaccaggcaa tcatctggct
    17101 gcatcgcctc aacaagccgg gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac
    17161 gtgcgacgtc ttctgcatga ccagcgaggt cggtttcctg gatattggtg caaataaacg
    17221 ccgtcggatg cgtgttaaat tcgtcaaggg tgatatccat aatccgctgg ctatccacca
    17281 ggttttgcca gctcacgcca taaggattgc tgacgatatc tgccacttta cctaccagca
    17341 cggttggcac gcccgcctca tacagttttt gcggcacctg cactttttca tcaacaccgt
    17401 agcccatatg cacgacctgg aaaccgttgt cataagcgcc agaacgcggc gcattgatgc
    17461 caataaagcg cccttctttg ctttctgcgg catcgagaat acgctgactg tcggttaaca
    17521 agccgccaaa tgtaatgacc cgaccgacct gtacctgctc acgcacgata cgaccaattt
    17581 tgattgcgtc gtcaaaagag atcacagaga gattggcggt aatgttatag acttggccta
    17641 aatccgcctc gagattatcg ccaatcgcaa ccgcctgatt gacccacaga aattgcagat
    17701 catcgccacg gcgctccacc tgccagccag cggaaactaa tgcctgctca acacggtcaa
    17761 tcacatcgcg aaaaggcatc cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat
    17821 gccccataaa ggtatcgcca ccttcatgtt gcagctctgc cacgccccag gttgcggaat
    17881 ctgacggctg catatcgcct ggcgcataac ccaatgcgtt gattagcccc agcttctcca
    17941 gcgttggtag ctgcaaatgc ggcaactggc tcaggatgtg accacaggta ttcgctcccg
    18001 catcttgcgg acgcaccagc gtgacatctt tcattgcccc tacgccaaag ctatcaatca
    18061 ctaacaccac aaatcgcgcc attacgcccc cagagagttt ccaagactgt cataacgacc
    18121 aacgattttc ggttcgccac ggtgaatacc ggacaccagc accacatcgc tacgggtgac
    18181 aaaaatctgc gtgcggaaac agagcaccac tgcactgctt accggaaact cgcctgccag
    18241 cgacagggtg tagtcgatac tgctgtcatc cacagttttg agattggttt cagtaatctt
    18301 ttgattttct ggcgtaaaca ccaacgcatg ttgcgcatga ccacgacggt agtaaccgcc
    18361 gccgtagcag tagctgtcgc cacggaaatg atgggagatt tcactcaacc agagcatcgc
    18421 gatacgttca gactgatcgc cctgctggtt tgccggaata gtgcccgtca gcgcatgacc
    18481 gggttcggca tgagtcacac cgtattgcgc cagtaatggc agcgaagtgc agctggtcgc
    18541 tgaaggcgcg ttcagttgct caatcacaat accagatttc gccagttgat cccgtgcttg
    18601 taccagcgtg tgaagattcg gtgtcggcaa aactttcccg gcagcctcat cccaaagcag
    18661 gcaagggaaa tgggtaagtc cggctaaatg cagccccggc agtttcttaa tttcagcaac
    18721 cacctcgtgc agcgaatgct gtacaaaacc gctctcctgc cccggataaa gaaaatcatc
    18781 gtcgctataa actttgagca gcacagactg gattcgcccg gccttcaccg ccgccgcaga
    18841 aacttcccgc gctttgtcga gagtaaacac ggtgatgacg tcggtgccct gttcaacggc
    18901 gtcagcaacc tgatgacaag ggatttgtac cagatgcccc tgatgcgcca ccggcaaacc
    18961 agcgcggcgc atgactcgcg cctctttgta atccaccgcc acaatgccgc tgtagcccaa
    19021 cgccagcagt ttttccgcca gccatgggtt acgaccgaat tgtttggtca tcagatacag
    19081 ttcaatgccg taaagccgcg ccgtctcaat cagccgctta ccgttttcca gtatctgatc
    19141 cacgtcgatc acccagctgt ccggggcgat tttgccctgc tgccacaaac ttagtgcggc
    19201 ggaaatcagc gccgggttct ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt
    19261 ctgttccttg aattaaatat tcattttttt gaatatttag gttgtgcgac gtggatttgt
    19321 cgggagcttc aaacctcttc gttagcagcc atccataatc cataaaggtt ggccagcaaa
    19381 tagccttctt cacacgggtt aacttccagt gcgaattcct tcaacaatac ctgatgcaat
    19441 tgcacaacag cttgccagtg gctggattgc gccagctctg ccagcaattc gttatccagc
    19501 ggctctattt cttcaccacg gcgactgcgc atcagtgcac tcgccatatg tgtaatcgcc
    19561 atcgttcctt gctcactgcg cactggcaga tggcactctg tttccagtac gttgacgacc
    19621 tgcatcatgc ctttgcagat gtccttatca ataacgcctg cctcgcaaag caggttgagt
    19681 ctggtttcca tatcagatgc ctttcgttta ataactcggt tcctgttccc cactcaccac
    19741 gcgttgttga tgggcgagca gggcgtgttt atctacaacg gcacgcagca gttgttgcat
    19801 tgggtaatca tcggctcgcg tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc
    19861 gtctgtgagc ggcgtcgcct ctaatcccag catggtcagt tcgttttccg ccaccacgtt
    19921 ccagataacc gcatcgacat cgcctttaac aatgcgttgt aaactctcgt gataagagag
    19981 atcgacacgt tccacatcac tatcgccaaa aaagacatcg gtcatgattt tctgatccgc
    20041 cgaacggtta tccagcccca cgcgcttcac gtttgcggac tcgcctttac ggcaaatcaa
    20101 ctggtgctcg ccaacgtagg tgtgcggccc caactccagc gcgaggcaaa accctttttg
    20161 cgtgagataa ctttccgccg ccagtcggga aaccaccgcc atgtcataca cgccgttaag
    20221 taaacactcc acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc
    20281 aaactgggct ttcaatccgc tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat
    20341 cgcacatacc acgttgttga tatccacatg actcagcaat gctttgttat ccatctcgac
    20401 cagataactg ccattgcgcc cacggcgttc aatccgtatc gctccgcttg attccagcgt
    20461 tttcaacgcg gcctgcgtca ggccaacgga tgaacggcat tcagttgcca gctcatctat
    20521 ggttttcaac ctattaccgc atttttcacc caacaaataa cgggccagcg tcgtgataac
    20581 gacgccttct tttttgataa acgttcgacg cataatatat tttcaataaa atgaatgttt
    20641 atatcttcat taaaatgaag ataagtggca actgtgagat gtggaaacgg cgaggcgctt
    20701 cacatttttt cggattattg caaatgagga taaaaaaacc gggtttcccc ggtttcagag
    20761 tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt aattcccact ggcgcttgcc
    20821 tgctcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccga gcgttttgat
    20881 attacggctt cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta cgggaagcgt
    20941 gtacactggc tttttctgcg ccgtctttca tcacctgttg caggaaggct tcatcgttgc
    21001 ggaaacggtg atagcgttcc tgcaattcag tcagcatacc ggaaacggca tcagccactt
    21061 cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct ggaatgctct
    21121 ggcccgttac cgcagaaagg atatccagca ggttggaaac gcccgctttg ttctgcacat
    21181 cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt ttcttcacta
    21241 ccgatttcgg atcttccagc aggccgataa cgttattgcg gttatcgtca gacttggaca
    21301 tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga ataaacggct
    21361 ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcgata tcgcgactca
    21421 gttccaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga tacagcagga
    21481 tgtccgcagc catcagcacc ggatagtcaa acagaccagc gttgatgttc tcggcataac
    21541 gcgcagattt atctttaaac tgggtcatgc ggctcagttc gccgaagtag gtatagcagt
    21601 tcagtgccca gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa atggtgcttt
    21661 tctcaggatc gataccacaa gccagataca aggccagcgt atccagcgtc gctttacgca
    21721 gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag taaatgcaat
    21781 ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta ccaatggtca
    21841 attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt cgattcctga
    21901 ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg ataaattaca
    21961 tcaggctggc tgagatcgat agcctcgccg tagttatatc cgtaggttaa gccaactgat
    22021 gggcaacctg ccgcttttgc cgcctgaata tcattgcgtg agtcgccgac aaacagcatc
    22081 tgttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg atgcggtttt
    22141 ttgttttgca catcatcgcc gccgataacc acgctaaagt atttggcaat atctaaggct
    22201 tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaggcctag cggcaggcct
    22261 ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt cccctcttcg
    22321 gcaacctcgc catagtagcg atcgaacagt ttacgcagaa tacgtacctg ttcttctgcc
    22381 ggaatgtcgt catcaacggg cggtttaccc attgttttac gctgagtcgc acgttcctga
    22441 cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat ccaggtaata
    22501 acgcgttctt cacctgcgac gggcaactcc agcgcataca gcgccatatc taccgcagca
    22561 gcaagaccgg gagcactgtc gaccagcgta ccatcaagat caaaagcgac gccgcgaata
    22621 tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat caatgacttt
    22681 tttgtagtct ggctggtcga agattgccga accggcgacg aacatatccg cgcccgccgc
    22741 agcgatttcg ccaatgttgt tcaccttcac gccaccgtct acttccagac gaatgtcaaa
    22801 gccagactcg tcgatacgac gacgtacttc gcgcagttta tccagtgttt gaggaatgaa
    22861 cgactgaccg ccgaaaccag gattgacgga catcagcagg atcacatcca gcttatccat
    22921 cacgtaatcc agatagctca gcggtgtcgc cgggttaaat accagtcccg ctttacagcc
    22981 attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg gatgaaaggt
    23041 aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacag gtttcaccat
    23101 caggtgtacg tcgatagggg cggtaatgcc atagttacgc aaggatttca gcaccattgg
    23161 cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca cgacatcagc
    23221 gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag ccgacagaat
    23281 tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt tttcgcgggt
    23341 gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg tgtgccgccg
    23401 ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg ataccactca
    23461 cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc aaccagacct
    23521 tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt gtgatacgcc
    23581 gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac gacggatgca
    23641 tcatctgcgc gcgccatgct atcggcgtaa gactcacaat agaaaaaggc attctgcgct
    23701 ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta gcggccaaac
    23761 ggcacattaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc gtagcggttc
    23821 aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa ctcttcgcgg
    23881 aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg tgcggcctgt
    23941 acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc gctattgata
    24001 tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc acctacaaaa
    24061 ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag caggggatac
    24121 ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgctgactaa ctaattacac
    24181 cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcaac tcccgcaacg
    24241 ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct gacgcagcgg
    24301 tttcgcccac gggtttttgg cctggacatc tgctggcaat gtagataccg cttttttcgc
    24361 ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac cattacgcgt
    24421 cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt tcaggttgcc
    24481 gtagttagag gaactgctca gctgcagagt gtaatggctg gacggtgccg atttcaacga
    24541 accaacatta cctgcggtct tccctccagc ggctggtgtt gccgtggttt gcgccgggga
    24601 tgccgtctgt actggagccg tagtcgccgt ctcttttgtt gctggcgtag aagtcgcagc
    24661 cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg tctgtgctac
    24721 cggcttcggc tccgttttca cggttgcttg cggttttttc ggttcaatca ctgcctgctg
    24781 gcgagctgga cgcgtagtgg ccggacgttc agcggtttgc gttttcgccg tttcacggga
    24841 tgcattgcca ttgcgaacag gcgcgacagt cgcgggttcg gtcggcaatg tggaattgac
    24901 cgccacattg ttcaactgtt gctgattttg tggttgggtc agcgcattgt tcaggtcacc
    24961 ctgcacttca acacgttgtt gaccatccgt tgccgccggg gtttgccctt gagtcggcgt
    25021 agaagagatc ggcggcagag aaacatcctg ctgagtattt tccgcagacg tggttcccgg
    25081 cgctggctgc acgccattgg cctgatcggt cgcattgcca gcaagatcaa tactcttctc
    25141 gccagacgcg gtttgatcgc tggaagaggt cgagggggct tttagcgcag aaccgatacc
    25201 gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact gacgagaagc
    25261 gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct cttcatccac
    25321 ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat tgcgctcttt
    25381 ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat caaaattgat
    25441 ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag aacgaccagt
    25501 acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat ccatttaaca
    25561 ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat agaccgcttg
    25621 ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg gcgttaagaa
    25681 caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac ggaagaatta
    25741 agcgcatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa tacgcctgcg
    25801 cggacatttc gcgcggccca ttgaccggta acccagcccg cgtgagcagg gttataatac
    25861 gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc gccatcacca
    25921 tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca gcttcaatgg
    25981 catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg cgctcgtcgg
    26041 cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc attgccggac
    26101 cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac gcaccgtcaa
    26161 gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc gggggaagcg
    26221 ttttcagaca gtcgagatcc accaccactg aagcaggctg gtagaacgcg ccaatcatgt
    26281 ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg acctgcgaca
    26341 gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc gccgcgaagc
    26401 cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg cgaccatgcg
    26461 gcttttgtaa caacgccgta aagacggtat cgagtacagc caggctttta tactgctcgc
    26521 cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct
    26581 tatcgagata cagaggagcc agggtttcgt tggtgaccaa catgacctgc tcgcccgatt
    26641 tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta attgggtaac
    26701 tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct taattactgt
    26761 acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt gaataatctg
    26821 gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg caatctcttc
    26881 atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg gtgtttcaac
    26941 gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt
    27001 ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac gggatttcac
    27061 agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat tgatgacctt
    27121 ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc
    27181 tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga gttgttgagc
    27241 taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa agatattgcg
    27301 tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac ccgcttcgct
    27361 cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag tcagactgaa
    27421 aattatctca atactccagc gggtttggca actgaataaa tcacccatcc ccctgcatat
    27481 ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc accgggtaag
    27541 attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc atggaacgca
    27601 ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata cctgcgcgcg
    27661 tcaaatagat gaaacacgtt cagcataaaa acattaccgc ttttacggct gtttactcac
    27721 tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct tcttttccgt
    27781 catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg ctatcctgac
    27841 ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg cttttgacct
    27901 cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg gcctgctgta
    27961 gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcga cctttttgta
    28021 acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc gacgtcgcgc
    28081 cactttcccc gctggaaacc tgatatggaa tttcgctccc ctgtttaatg ctggcaggct
    28141 gaagatgtga ggccagcaga cgcggactgg cgataatatc cagctgctgt ttttgttcga
    28201 gcgcagaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta aaaccgacat
    28261 gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc ccaacgccac
    28321 cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa cttttttcat
    28381 taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc atttgcgcta
    28441 cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg cgattggtgc
    28501 gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct ttcgccagtt
    28561 ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga tttgcctgcg
    28621 cccgcgcctg ctccgcctcc tggcgggcga tattgttatt ctgccaggca atggaatgca
    28681 ctgagagaat gttgtcttcc tgccgcgtta tcagtccggc gcttttcact acagtttgta
    28741 gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg acgtctggcg
    28801 acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga gctaccggaa
    28861 cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg ggtatcagca
    28921 tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg ccacaaccat
    28981 tgtggcggtt cgcagtttgt cccggttccc agcgttagtg cgtctggcgt cagctgtaaa
    29041 attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt cttttgcccg
    29101 tcttttatta caccgatgat gcgctcgcct cgccctacca tcccctgata gcgccactgg
    29161 ctaagttcgc taatccggca tagatcttcc ggcggtttaa aagggtcacg cataccggtt
    29221 aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcgtctccag
    29281 ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac
    29341 gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc
    29401 caactcacct ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa
    29461 atccagaggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg
    29521 atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc
    29581 ttcatggtgt gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca
    29641 gcaaagctgg cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc
    29701 taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg
    29761 ccccccgctg attgagatga aaagaagcat cctggcgaag tgacgtttct agtgcgttta
    29821 acgcggtaat gcttgttgta agccccttga tctccagcgt tccctgctgc cagcttatcg
    29881 ttgtcagcca ggcgtgttca ggtaaaagag ccgccagtgc ttccagcgca gattgccagt
    29941 cgcgggtaaa ttgtcgctgg cgctgccttt gcaaacgctg ctcacgaatt tgttggcgct
    30001 ccagcaaacg tggtttcgtt atctgtaagc tgtgggcgag ttgttgttcc ccctgaagca
    30061 aaacggcgtc tacgcgagct tcggcgctgc ttgtcagacg cagtattagc gttatcccga
    30121 cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc
    30181 gttgctgtcg ccagggcaaa aaattaattg gcgggttcat cagtacgcct tcccaagcgc
    30241 cagccccagc gcgatggcaa agtctccacc tgacggcggt agcggcggct gacgaacaga
    30301 aacggcctcc cagggatcaa atcccccttc gccacatatc gcgacgcttt cgggatcaac
    30361 ggataacgct gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca
    30421 gcgatagcgt gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg
    30481 agaaggtaaa aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac
    30541 atgaacacgc aacctttctg ccagagtaag cagcgttgcc agctctttgc tttgcgcggc
    30601 agtcacgtta taagcggggc tgagtgagtc ttcgctataa tcgaagcgca gggagtccgg
    30661 atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc
    30721 aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa
    30781 cataatgtga tgacgctgcg gcagttcgcg actccaaggt aacaacgttt tagccagctg
    30841 ctgcgcatca acaatccgcc catctttgat aatgtcgttc tccagcggca accgccacca
    30901 gcgttgcaaa aagcattctt ttgcgccccg tacgatcgca accgctaccg cttcttgctg
    30961 ttgtaaatgc aaaccaattt gccagatctt aaatgccatt gtgatgatct ccttatcacc
    31021 cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt
    31081 tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt
    31141 tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct
    31201 acatcgagcc acaactgccg gatgtggcga cattaaaaga tgttcgcctg caaattccga
    31261 tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc
    31321 cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca
    31381 gccgcttcta cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg
    31441 cgctgttctc cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa
    31501 atttcttcct cagtccagaa cgcacgctga tgcgtaagat taaggaagtc ttcctcgcga
    31561 ttcgcattga acagttgctg acgaaagacg agatcctcga gctttatctg aacaagattt
    31621 acctcggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggaaaaacgg
    31681 tcgaccaact gacgctgaac gaaatggcgg tgatagccgg gctgccgaaa gcgccttcca
    31741 ccttcaaccc gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc
    31801 ggatgctgga tgaagggtat atcacccaac aacagttcga tcagacacgc actgaggcga
    31861 ttaacgctaa ctatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg
    31921 tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt
    31981 acaccaccat cacccgcaaa gtgcagcagg ccgcacagca ggcggtacgt aataacgtgc
    32041 tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg
    32101 agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgcca acctatggtc
    32161 cgctgctgcc tgccgcagtc accagcgcca atcctcagca agcgacggcg atgctggcgg
    32221 acgggtcgac cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg
    32281 atactcagca aggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa
    32341 tctgggttcg tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg
    32401 cgctggtgtc gatcaatccg caaaacggtg ccgttatggc gctggtcggt ggctttgatt
    32461 tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca
    32521 aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg
    32581 atgtgccaat ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac
    32641 caccgcagta tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg
    32701 tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct
    32761 tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct
    32821 tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggtggc ttcctggtgg
    32881 acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac
    32941 cgaaagtagc ctgcccggaa tgcgatattc cggtgattta cggtgatacg cagaaatcga
    33001 acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt
    33061 ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc
    33121 aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga
    33181 acaccaatat ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt
    33241 tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt
    33301 tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc
    33361 gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag
    33421 gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg
    33481 tgccggagca gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca
    33541 ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc
    33601 cgacacaaca ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg
    33661 aattgttctg attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg
    33721 tttatgccgg atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata
    33781 ttgcaggaaa cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca
    33841 ctcgcccctg ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt
    33901 cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg
    33961 gtagcggctc tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga
    34021 tattcatttt gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat
    34081 cgttcgcacc aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt
    34141 cacccggatc aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt
    34201 attcgcggat caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt
    34261 tggttggacg cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc
    34321 tctcgacggt aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt
    34381 gtaatgattt gctcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg
    34441 gacacgcttt cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat
    34501 cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt
    34561 ccgccttcag agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag
    34621 ttaaactcca cgcttgccga tagccaaccg cagaatcatg tattgtgtct ggtgcgactg
    34681 accacgcctg acagactaag taagatgggg aaagcatgag caccattgtg atttttttag
    34741 ctgctttgct ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc
    34801 gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag
    34861 aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc
    34921 caacgggagc cagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca
    34981 tgatgctgac acacgctatc acgcgttacg gcatctctac cgacgatccg aataaatggc
    35041 gttactacct cgattcggta gaagtccacc tgcccccttt ctgggaacaa tacatcaacg
    35101 atgagaatac cgttgaactg atttataccg attcgctgcc gctggttatt tcactcaacg
    35161 gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt
    35221 ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca
    35281 aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga
    35341 tcgtcgcctc cttcctgatg ttctttttct gcctgattac cccagatgta tttgttccgt
    35401 ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc
    35461 cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat
    35521 tgtttggcga aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg
    35581 tctatcccgc acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg
    35641 atatctatct cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag
    35701 taaaaaactt tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc
    35761 tggtgctgtt tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct
    35821 catggatgaa aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg
    35881 gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt
    35941 ccggaacctg gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca
    36001 tctggaatga cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg
    36061 cattgactga agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca
    36121 gtgcctcatt acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg
    36181 acattgtact gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa
    36241 atgcgctggt caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg
    36301 ccgggaagct cgatggcgtg aatgtgttat tacgcccggt gagtgcagaa tcgctggata
    36361 acctggtggc aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat
    36421 cactaaacag tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg
    36481 ttgatcagcc ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg
    36541 ctttccagaa actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca
    36601 caaaaatctt cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc
    36661 gttccggcct gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg
    36721 ccatttacaa tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg
    36781 agattcaggc ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc
    36841 ttatcgaata acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact
    36901 ctggagattt ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg
    36961 gatatggacg gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg
    37021 cctgaaacat ggggcgccaa aaacggggtt acgccacagg aagcgatgga atatatgcgc
    37081 cagcaatatc acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag
    37141 caactgggtc tggatatctg tgcgatgacc accgagatgg gaccgcgtgc cgtactgcgt
    37201 gaagatacca ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc
    37261 accaatgcgc atccgcacaa cctggcggta aaacttgagc ataccggtct ggacgcacac
    37321 cttgatttat tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg
    37381 catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc
    37441 gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat
    37501 cctgattccg ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc
    37561 cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt
    37621 tcgactggat aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga
    37681 aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt
    37741 cgagctgaat gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa
    37801 ggcgattact gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc
    37861 ggaaagtgta gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat
    37921 gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca
    37981 cggcgacagt gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt
    38041 acatcgctat ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac
    38101 cctgcaacag atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga
    38161 actgctggtt gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt
    38221 acagctgcaa ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca
    38281 aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct
    38341 ggtcggcaat ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg
    38401 cgtggttggt ctggaaggtg ataccctggc ggcctgcctg gaagattact ttatgcgttc
    38461 tgaacagctg ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc
    38521 aggcggtatg ttgttgcagg taatgcctgc gcaaaatgcc cagcaggacg actttgacca
    38581 cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga
    38641 agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga
    38701 gttcaaatgc acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga
    38761 agaagttgat agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg
    38821 taaccactat ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc
    38881 agatccgcaa gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac
    38941 gtaatggcct gatgtgctgc gcttaccagg cctgtgaaat aaatcgcaaa ttattgttgg
    39001 tcagatacag cgtcgtatcc gttaaaagag ttgtcactta cttaagttta tttccagcgt
    39061 ttcaccatct accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt
    39121 cgcagctgtg agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc
    39181 gtcccattct tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc
    39241 aaacaggatc cgctccgcct gaccatcact attcagatct tgctcgacca ggacgcaggc
    39301 gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt
    39361 cgcatccggt ttaacagaac caggggcaat taacacattt tcggctaata ctttttccga
    39421 tacctgttcc tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt
    39481 cgggtctttc ataaacccgg catcgctttt cagcgactca agcgcagcac gtccatagcg
    39541 accgctctgc tcgagcatgt agatagttac ctggtctgac gtgtttttgc cgctctgata
    39601 acgcgccata tggctgttca cgctaatgcg catactgtcc agcaccggcg aattaagaag
    39661 caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaac
    39721 gggattttgc cctttacgcc agacgatgct gacaaaatac cccagcgacc acaccagtaa
    39781 caccagcacc gccagcacgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg
    39841 cagccataac gcccaggcgg cgacgaacac atacagcgga gcaaccagca aagcggtttt
    39901 aatcaggcaa cgcaacggcc ctgtccaggg aagtgacgct ttttgcggat cgcggacaat
    39961 agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg
    40021 gcgagaaatc gcgctcaggc ccgtaaacgg cagagtgatg ataaacatca gggttagcaa
    40081 tgatactaac ggcagcaacc ccgtggcgat taacgtgaac aacttttgaa tagagtcgat
    40141 taaacgtgac tgtgttcgcg cgaggatcac cgccagcgcg gtaaccagac ctaacgtgag
    40201 ataaataaac cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt
    40261 aaacaactca ctccacagta aaagcaccag ccacgtcaag ccattggcga gaaaaatcac
    40321 cagtaatata agtacgttat gccatactga ctggtaaaaa tagcgatagc ggctactgtc
    40381 attgcggatg cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcagcagcag
    40441 atagcaacca aaatcccaaa gcgccttttc agctctccag gggttcatgc catcagtttg
    40501 ccacttcagc cagccgctca tccccagcgt ggcaataaac accagcgcca gccatcccca
    40561 aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcaacggt
    40621 cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg cgatcaacag
    40681 gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga
    40741 aagttcaacg ttatccatgt tggttatcca gaatcaaaag tgggttaatt atcgcatccg
    40801 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    40861 actttaccga ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc
    40921 ccgccatata aactaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    40981 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    41041 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    41101 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    41161 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    41221 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    41281 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    41341 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    41401 aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg
    41461 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc
    41521 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    41581 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    41641 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa
    41701 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    41761 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    41821 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    41881 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggtgatg
    41941 acgagcacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    42001 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc
    42061 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    42121 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacattgtt aagccggttt
    42181 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    42241 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    42301 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    42361 tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    42421 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    42481 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata
    42541 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    42601 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    42661 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    42721 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    42781 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    42841 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    42901 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    42961 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    43021 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    43081 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    43141 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact
    43201 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    43261 atcacctcac cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    43321 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    43381 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    43441 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    43501 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctccgaagcg
    43561 ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    43621 agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    43681 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    43741 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    43801 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg
    43861 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    43921 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    43981 tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg
    44041 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    44101 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    44161 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    44221 ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca
    44281 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    44341 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    44401 ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac
    44461 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    44521 gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt
    44581 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    44641 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    44701 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    44761 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    44821 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    44881 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    44941 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    45001 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    45061 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    45121 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    45181 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    45241 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    45301 atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac
    45361 tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat
    45421 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    45481 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggct
    45541 gtggtgaata ctcctgccgg ggaagcgagc tggtatgtta atgctatcga gtacgtgaaa
    45601 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    45661 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    45721 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    45781 ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    45841 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    45901 gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat
    45961 ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc
    46021 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    46081 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgcggctgc acaatatgtt
    46141 gatgccgata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatcctg
    46201 atggaacgtt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    46261 aacgcgcatc tggtctcaac cgtggtgagc ggtaaagaag aggaaggggc aaaattccgc
    46321 gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg
    46381 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgat
    46441 gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg
    46501 aacaatgccc cggcggatag ctggcgcaaa ggcgtagtga gctggacctg gcgcatcaag
    46561 gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa
    46621 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    46681 cgtgcaacga tgggcctcga tccgggtctg cgtactgggg taaaagtggc ggtggtcgat
    46741 gccactggca aactggtagc gaccgatacc atttacccgc acaccggaca agccgcaaaa
    46801 gcagcgatga ctgttgctgc gctgtgtgaa aagcataacg ttgaactggt ggcgatcggc
    46861 aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg
    46921 aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc
    46981 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc
    47041 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    47101 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta
    47161 gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta
    47221 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    47281 aacggccagt tcaagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    47341 ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct
    47401 accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    47461 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    47521 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    47581 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    47641 gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa ctttggcgcg
    47701 tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    47761 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    47821 gaagtggatc ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc
    47881 gaaaccaacg cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    47941 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    48001 gcggcaatgg gcaaaaaacg ttaaacgccc tgattgaccc ctaaacctgc gcgataaggt
    48061 gtccacgccg catctggcaa cgaacgcctt gtctgacata aaagtgccgg agaatatctc
    48121 cggccttttt attccacaac caaactcata atatattccg gcaatattta tcacttcatt
    48181 aacaactgaa accttaatta aacattagcc agtccgggta attcactatt cgaattatat
    48241 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    48301 cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgttgttg
    48361 attatttaat ctctcctttg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc
    48421 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    48481 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    48541 tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg
    48601 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    48661 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    48721 ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    48781 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    48841 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    48901 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    48961 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    49021 ctacaactgc tggaactcgg cattccctgc attgtggcac tgaacatgct cgacattgcc
    49081 gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccagtg
    49141 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    49201 tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca
    49261 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    49321 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    49381 ctggatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc
    49441 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    49501 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    49561 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    49621 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    49681 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    49741 ggcattaaca ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc
    49801 ttccttgagg actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag
    49861 gcgctgggct tgccggggaa atcctttgtg ccgttgatcg tcggtttcgg ttgtaacgta
    49921 ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    49981 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    50041 ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg
    50101 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt
    50161 gtcatggagt tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg
    50221 cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc
    50281 ctgagcgctt tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg
    50341 gcgctggctt ccgtcagccg ggtgatcacc ccggtcttca agccgattgg cgtacatgaa
    50401 gataactggc aggcaacggt tggcctgttt acgggcgcaa tggcgaaaga agtggtggtg
    50461 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggcg
    50521 gaattcaacc tcggtgaaga gctgttcagt gcggtagatg aaacctggca gagcctgaaa
    50581 gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg cgacggcgaa
    50641 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc tgccgcatac
    50701 agctacctga ttttcgtcct gctgtatgta ccgtgtatct cggtgatggg ggctatcgcc
    50761 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    50821 tcactggcaa cattgttcta tcaggtcgcc agctacagtc agcatccaac ttacagcctg
    50881 gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg
    50941 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    51001 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg
    51061 cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa
    51121 tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag
    51181 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    51241 gcgagtggtg ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc
    51301 gtctgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg
    51361 cgtaagttcc tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggagtgaag
    51421 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag
    51481 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt
    51541 gattttcttg cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc
    51601 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    51661 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    51721 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    51781 caccttgatg ccggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    51841 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    51901 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    51961 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    52021 cgcgacctga tggggcaggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    52081 gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    52141 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca
    52201 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    52261 atgcttgaat ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa
    52321 gagttggctg cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc
    52381 tctgcttcaa cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat
    52441 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    52501 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatgggca
    52561 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    52621 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    52681 gcgccgtttc agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    52741 catcactgag ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt
    52801 cacgagcact aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat
    52861 gggttaacgc aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    52921 tatcaggtgc ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc
    52981 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    53041 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    53101 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    53161 tgacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    53221 cactggcgtt aggtcattgg gggatttgtt cggtctgctc acgcgctgcc cgtacagata
    53281 aaacgttatg cccacaatgt ggattacccg ccacacactc acatcttccc tgcggtcgct
    53341 gcctgcaaaa accaccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt
    53401 taagtccgct tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt
    53461 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg
    53521 atcgcatcat cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga
    53581 gcgatttgct gtgtcagccg ttatcacgct ggttgcactg ccgatgggat agcaaagccg
    53641 tcacacgaac acgggccact gcgacccagc attttctcag tgcccggcta cgcaagcgca
    53701 acctgaaaaa tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg
    53761 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    53821 gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc
    53881 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    53941 gctgcacaag cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc
    54001 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg
    54061 gatgccgtgg aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat
    54121 gagttaagcg caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt
    54181 tctcagttaa cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg
    54241 ctgatggagc gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac
    54301 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc
    54361 ggcggctgta acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag
    54421 ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc
    54481 gaacactcct actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc
    54541 gtcatttcgg gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat
    54601 ccctcccttc gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc
    54661 tctggtgatt ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact
    54721 cctgacatct gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct
    54781 ctgttcccga ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa
    54841 tgaaaggcca ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg
    54901 ctcctgatga tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct
    54961 gttggattaa tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc
    55021 ggcgggacgc tcggtagcct tgccctgatc atgggttttg gcgcaatgct gggcaaaatg
    55081 ctggcagact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa
    55141 aagcacatcc agtgggcggt ggtgttaacc ggttttaccg ttggttttgc cctgttctat
    55201 gaagtgggct tcgtgctgat gctgcctctg gtgtttacca tcgcggcttc cgcgaatatt
    55261 ccactgcttt atgttggtgt accaatggcg gctgcactgt ctgtgaccca cggcttcctg
    55321 ccaccgcatc cgggcccgac tgcgattgcc accattttca atgccgatat gggtaaaacc
    55381 ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct
    55441 cgcgtgttga aaggtatcga taagccaatc ccggaaggtc tctacagcgc gaaaaccttc
    55501 agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggtagta
    55561 ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg
    55621 gtagcggagt tcctcggtga cccggtaatg gctacgctga ttgccgtgct gattgccatg
    55681 ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct
    55741 tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag
    55801 gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt
    55861 tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca
    55921 accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt
    55981 agcccggagc tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac
    56041 gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa
    56101 tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg
    56161 aatatggtga tttgataaca ctgactgccg gatgcatcgc atccggcatc aaccgcactc
    56221 tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcacg
    56281 ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac
    56341 tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt
    56401 agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg gttcagcgtc
    56461 ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta
    56521 tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt
    56581 accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag
    56641 taaccgcgta gcgtaggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac
    56701 gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag
    56761 tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca
    56821 ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc
    56881 accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa
    56941 cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc
    57001 aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt
    57061 aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgacgctttc
    57121 aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac gccaaccgcc
    57181 agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca
    57241 aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct
    57301 tcgcagaact gacgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc
    57361 cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc
    57421 tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggt catctgctca
    57481 tgatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta
    57541 accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc
    57601 ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac ggcgttaacg
    57661 tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactctcc
    57721 ggatttgccg gatagagcgc atgaatcggg ttcaggccga tgaacgaccc gccacgtttt
    57781 gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc
    57841 gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc
    57901 tgcggttcgt aacagcgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc
    57961 tgggtgagtg tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg
    58021 ccccccgtta catggccttt gtactgtgtt ccttcttcgg tggtcagcag ccagctatat
    58081 tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc
    58141 gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc
    58201 cgtttggttt cggcgctaat cgactgcggt ttaccgtagg cattgatgta attggggcta
    58261 atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt
    58321 tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg
    58381 cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca
    58441 tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag atacggatcg
    58501 ccgccttgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg
    58561 tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat
    58621 ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt atgaccaaaa
    58681 ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc atccagcgta
    58741 ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta
    58801 cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcagtttttc cgccgccgaa
    58861 acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg atcgttgttg
    58921 atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt
    58981 gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga
    59041 atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc
    59101 agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc agtacgaact
    59161 ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg
    59221 aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac
    59281 tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg tttgatccag
    59341 cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat
    59401 tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg
    59461 aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt
    59521 ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta
    59581 ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacatcc
    59641 cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggta
    59701 atggcccagg cgtcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct
    59761 ggaatcgcga tagttgggtg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg
    59821 tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga acaggcacac
    59881 tggaagtact gctgcatcaa gcgcagcttt ttaccggcag tatggttgtc gtttggatag
    59941 agaactttgg tcagtttttc cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg
    60001 tcgttaaatt tagtcagatc aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc
    60061 tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta
    60121 aactccggct cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc
    60181 agtgcttcgt tgtggcggaa ccacgggtag ttactgcggt gccagtcatc cggcgcttca
    60241 acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa
    60301 cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc
    60361 agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtccgt cagattgatg
    60421 tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg
    60481 cccgtcaggc gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc
    60541 accggcttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgctagc
    60601 caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt
    60661 gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt
    60721 ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc
    60781 atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcaa
    60841 ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgcaata cagcgcagaa
    60901 aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc
    60961 tataaattaa gtaaaggagt gaaacagttt cataagtaaa atatccagtg tgctccatct
    61021 cattcttaat agatttatta agatcatctt tttagatggc actttcatca ggaatgaaga
    61081 agaaaccctt gcttaaatga atctgatgaa cataagggaa accagtattc acgctggatc
    61141 agcgtcgttt taggtgagtt gttaataaag atttggaatt gtgacacagt gcaaattcag
    61201 acacataaaa aaacgtcatc gcttgcatta gaaaggtttc tggccgacct tataaccatt
    61261 aattacgaag cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt gattaactat
    61321 gctgattccg tcaaaactaa gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg
    61381 cctgctggct aaactttccg gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc
    61441 gggctacgga aagaccaccc tcatttccca gtgggcggca ggcaaaaacg atatcggctg
    61501 gtactcgctg gatgaaggtg ataaccagca agagcgtttc gccagctatc tcattgccgc
    61561 cgtgcagcag gcaaccaacg gtcactgtgc gatatgtgag acgatggcgc aaaaacggca
    61621 atatgccagc ctgacgtcac tcttcgccca gcttttcatt gagctggcgg aatggcatag
    61681 cccactttat ctggtcatcg atgactatca tctgatcact aatccagtga tccacgagtc
    61741 aatgcgcttc tttattcgcc atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa
    61801 ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgat caactgctgg aaattggcag
    61861 tcagcaactg gcatttaccc atcaggaagc gaagcagttt tttgattgcc gtctgtcatc
    61921 gccgattgaa gctgcagaaa gcagtcggat ttgcgatgac gtttccggtt gggcgacggc
    61981 actacagcta atcgccctct ccgcccggca gaatacccac tcagcccata agtcggcacg
    62041 ccgcctggcg ggaatcaatg ccagccatct ttcggattat ctggtcgatg aggttttgga
    62101 taacgtcgat ctcgcaacgc gccattttct gttgaaaagc gccattttgc gctcaatgaa
    62161 cgatgccctc atcacccgtg tgaccggcga agaaaacggg caaatgcgtc tcgaagagat
    62221 tgagcgtcag gggctgtttt tacagcggat ggatgatacc ggcgagtggt tctgctatca
    62281 cccgctgttt ggcaacttcc tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc
    62341 ggaaatccac cgtgccgccg cagaaagctg gatggcccag ggatttccca gcgaagcgat
    62401 tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc
    62461 ctggagtttg ttcaaccata gcgaactgtc gctgctggaa gagtcgctta aggccctgcc
    62521 gtgggacagt ttgctggaaa atccgcagtt agtgttattg caggcgtggc taatgcaaag
    62581 ccaacatcgc tatggcgaag ttaacaccct gctagcccgt gctgaacatg aaatcaagga
    62641 catcagagaa ggcaccatgc acgcagaatt taacgctctg cgcgcccagg tggcgattaa
    62701 cgatggtaat ccggatgaag cggaacggct ggcaaaactg gcactggaag agctgccgcc
    62761 gggctggttc tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa
    62821 aggcgaattg acccgctcac tggcgctaat gcagcaaacc gaacagatgg cacgccagca
    62881 cgacgtctgg cactacgcct tatggagttt aatccagcaa agtgaaattc tgtttgccca
    62941 agggttcctg caaaccgcgt gggaaacaca ggaaaaagcg ttccagctga tcaacgagca
    63001 gcatctggaa cagctgccga tgcatgagtt tctggtgcgc atccgagcac aactgttatg
    63061 ggcctgggca cggctggatg aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc
    63121 gtcttatcag ccacagcaac agcttcagtg cctggcaatg ttgattcaat gctcgctggc
    63181 ccgtggtgat ttagataacg cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa
    63241 tggcaaatat cacagcgact ggatctctaa cgccaacaaa gtccgggtga tttactggca
    63301 aatgaccggc gataaagccg ccgctgccaa ctggttgcgt catacggcta aaccggagtt
    63361 tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct
    63421 gggcgagttt gaacctgcgg aaattgttct cgaagaactc aatgaaaatg cccgcagtct
    63481 gcggttgatg agcgatctca accgtaacct gttgctgctt aatcaactgt actggcaggc
    63541 cggacgtaaa agtgacgccc agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac
    63601 cggatttatc agccattttg tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct
    63661 gattcaactc aatacgctgc cggaactgga acagcatcgc gcacagcgta ttctgcgaga
    63721 aatcaatcaa catcatcggc ataaattcgc ccatttcgat gagaatttcg ttgaacgtct
    63781 gctaaatcat ccggaagtgc cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg
    63841 gcaggtcctg gggctgattt actccggtta cagcaatgaa caaattgcgg gcgaactgga
    63901 agtggcggca accaccatca agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc
    63961 ccaccgtcag gctgccgtgc agcacgcgca gaaactgctg aagatgatgg gttatggggt
    64021 gtgagtttag ccggataatg cattggatcc ggtttacatc tttacatcat tcaatactca
    64081 cccgcgttac gccatctgtt tctatcaaac caaaccgcac cggcaagaaa cgctccacca
    64141 ccgcgatatt ggtcagcaga tggcatgaag gatgggcgac cttaaattct cccgcgcccg
    64201 ccagcgccat cggcagtacc agttggtcgg cgagatattc ccccaccgcc gctgggcttg
    64261 ccaggtagcg tttcacctct ttcactaact gcgcggctac cacctcggca ctgacgcgct
    64321 tttcaccgac gacgaaaaag cgttcagtga tattttcact ttcgacttcc agcgagacgg
    64381 tattacccgg cccctggtcg cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac
    64441 tccccgccag tgtagcgatt tcacgctcag caacatggcg cggcacacca gctaatagaa
    64501 cctctccacg catctgcaca atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg
    64561 ccacgggcga gacttccgtt gccaccacgc cgcctccggc aggataaaaa ccgtgacgta
    64621 acagcgtggt ttgctgatga attcctattt tcgccagcag cggctccagc acccggcgga
    64681 taaaatccgc aggcggggcc gacgggttat cggtgcctcc gctcacttca acacgcgaag
    64741 gtccatcggc aaaccacagc gcgggcagca ccgtttgcag caccagcgta cagcttccgg
    64801 cactaccgat agcaaagcgg tactcgccgc cgcgcacgat gccaggccgg aagaccagac
    64861 gctgcgaccc cagctccgcg ccttccaccg ttgccctaca aatttccgca gccgctttta
    64921 ccgcggtcag atgctggcgc aacagccccg gtttcgcccg cccggcacga atgctggtga
    64981 tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc
    65041 caccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt catcatttat ccttttacgc
    65101 acaccacctg acgcagggta tagataactt ccaccagatc gctttgtgcc gccatcaccg
    65161 catcaatatc tttatacgcc atcgggattt cgtcgatcac ttcggcatct ttacggcatt
    65221 ccacatgcgc ggtggcacga atttgatctt ccacgctgaa cagttttttc gctttagtac
    65281 ggctcattac ccgcccggca ccgtggctgc acgaacagaa agattcttca tttcccagcc
    65341 cacggacgat aaagcttttt gctcccatcg accctggaat aattccatat tgaccagcac
    65401 gcgcagacac cgcgccttta cgcgtcacgt agatctcttc accaaagtgc tgttcttttt
    65461 gcacatagtt gtggtgacag ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg
    65521 ttttctgcgt aatgctctgc aatgccgtta ccacgttttc catcatcgca tcgcggttaa
    65581 ggctggcaaa aagctgcgcc caggccacgg ctttcaggta atcatcaaag tattccgtac
    65641 cttccataaa gtacgccaga tcacgcgacg gcaacgtctc aagcgtttcc tgcatctctt
    65701 tttgtgccag atcgataaag taagtcccga tggcgttacc aattccgcgt gaaccggagt
    65761 gcagcataat ccacacctgg tccgactcat caaggcagat ttcaataaag tggttaccgg
    65821 ttcccagcgt tcccaggtgt ttatagttat tggtattcag gaaacgggga tatttttgcg
    65881 ttaaccactg ataaccggct tcaagctcag cccatttcgc atcgacgtta acaggcgggt
    65941 tttcccatgc gcctttatca cgtttacaac ggccagtggt acgcccgtgc ggcacggcca
    66001 tttcaatcgc ctgacgcagc tctgccaggt tttcaggcag gtcttccgcc gttaacgcgg
    66061 tacgcagcgc gttcattcca cagccaatat ccacgcccac cgccgccgga ataatcgccc
    66121 ctttggtcgg gatcacgcta ccaatggtgg aacctttacc caggtgtaca tcaggcatta
    66181 ccgcaatatg tttgaaaata aacggcatct tcgccgtatt aataagttgc tgacgcgcat
    66241 cggcttctac cggcacgcct ttggtccaca tttttaccgg ggcattttca gtggtcagta
    66301 attcgtaatt cattttgttt ttctcttttc gttgtttgct gtcgtgataa ttgcaaccgt
    66361 cgtgccagaa aattaaataa gcagattaat tttttaattc attgttttta aataggttaa
    66421 atctttacgt ccgtaaacgg agattttcca caaaaccaat tcaccgataa tgaaatatcg
    66481 tcttttataa ggatatctaa gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg
    66541 ctggattatg ccggacgcgg cagtcagcgc tggtcgaaat ggcgtccgac gctttgttta
    66601 tgccagcaag aatcgttagt catcgatcgg ctggaattgt tgcacgacac ccgctcgcgc
    66661 tcgctattcg aaacgcttaa acgcgatatc gccagcgttt cgccagaaac agaagtcgtg
    66721 ggcgttgaga ttgaactgca taacccgtgg gatttcgaag aggtttacgc ctgcctgcat
    66781 gatttcgccc gtggttacga gtttcagcca gaaaaagaag actatttaat tcacatcacc
    66841 accggcaccc acgtcgcgca gatttgctgg tttctgctgg cggaagcgcg ttacctgccc
    66901 gcccggctga tacaatcttc acctccgcgc aaaaaagaac agccgcgcgg cgcaggtgaa
    66961 gtgacgatta tcgatctcga tttaagccgt tataacgcca tcgccagccg ctttgccgag
    67021 gaacgccagc aaacgcttga ttttcttaag tccggcattg ccacgcgtaa cccccacttc
    67081 aaccgcatga ttgagcagat cgaaaaagtg gcgatcaaat cccgcgcgcc gattctgctt
    67141 aacggtccaa ccggcgcggg caagtcattt ctggcgcgac gcatcttcga gttaaaacag
    67201 gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat
    67261 accgccatgt cgacgctgtt tggtcatgta aaaggcgcgt ttaccggggc gcgggaatct
    67321 cgtgaaggtt tattacgcag cgccaacggc ggaatgttgt ttcttgatga gattggcgaa
    67381 ctgggcgcag acgaacaggc aatgctgcta aaagccattg aagagaaaac cttttacccg
    67441 tttggcagcg atcgccaggt gagcagtgtt tttcagctta tctccggaac ggtgcgcgat
    67501 ttgcgccagc gggttgccga aggcaaattt cgcgaagatc tgtacgcgcg gatcaatctc
    67561 tggaccttca ccctgccggg tctacgccag cgccaggaag atattgaacc gaaccttgat
    67621 tatgaagtgg agcgccacgc cacactcact ggcgacagcg tgcgttttaa caccgaagcg
    67681 cggcgcgcct ggttggcttt tgcgacctct ccccaggcaa catggcgcgg taactttcgc
    67741 gaactttctg ccagcgtcac gcggatggcc acctttgcca ctagcggacg catcactctg
    67801 gacgtggttg aagatgagat aaaccgtctg cgctataact ggcaggagag ccgcccctcc
    67861 gcgcttacgg cgttgctggg cgcagaggca gaaaacatcg acctttttga caggctgcaa
    67921 ctggaacacg tgatagctct ctgtcgccag gcaaagtcgc tttccgctgc cggacgcctg
    67981 ctttttgatg tttcgcgcca gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa
    68041 tacctggcgc gttttggtct gacgtgggaa gccgtgcagg atcagcacag ctccagttga
    68101 atatgatggt ccgtcagcac ctgcattacg cttactggcg gcggggcgtc ggtgtagacg
    68161 gcatctacca tgctgatgct gcccatattg accatcgcgt tacggccaaa tttcgagtga
    68221 tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgcttggt gcgaacttcg
    68281 tggtaatcga actccagcag cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg
    68341 aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgccaatgat cccgccatcg
    68401 cggctgcgta attcgccacc ggcgagaata atgcggaaat cttcttttac catcaacgtg
    68461 ttagcaacat tgagattgtt ggtgacaata cgcaaattgc tgtgattaag cagcgcgtgc
    68521 gccaccgctt ccggcgtggt gccgatatcg ataaacagtg tcgagccgtt ggggatttgc
    68581 tccgccactt tgcgggcaat cctctctttt tcttcggtct gggtggcttt gcgatcgtgc
    68641 cacggcgtgt taaccgaact ggaaggcagc gccgcaccgc catggtgacg caggatcagg
    68701 ttttgctccg ccagctcatt gaggtcgcgg cgaatagtct gcgggctgac ggagaaatgc
    68761 tctaccagct cttcagtact gacataaccc tgctgtttaa ccagttcgat aataccgttg
    68821 tgacgttgtg tttgtttcat ttataaatcc ctggaattat tttcgttttc gcgcattgag
    68881 cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc
    68941 catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg caaagataat
    69001 taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcaccacgta gccagacgta
    69061 gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc tgaatttttg
    69121 ctgcacatag ccgcttaaca gggcgctaat gagagtaata acaattagct taccgctacc
    69181 gaggcgtttt tccaccgcac cgccgagata ccaccaccag agcaggttaa agaggatatg
    69241 catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt
    69301 tggatcgaat ggccaggcca gccataacat cacttcctga tcgccgagaa tttgcatggc
    69361 gataaacacc accacgcagg cgatcatcac cacccaggtt accggacctg cgcgttcacg
    69421 caaggcggca aagaaaggat aacggcgata atgcaggcca ctgccggtat ggcctgcctg
    69481 ccagctggcc gccagataac gcggatctgc cgggttttcg agaaaacgcg ccagctccgc
    69541 ccgtacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt tatgttgttg
    69601 aatcgtgagg ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg
    69661 gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac aaaaggggac
    69721 agtataaagc gttacgcgcc gtacgccacc tctgcgggaa actgacgttg ccaggcttca
    69781 aagccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata ctgcgccgcg
    69841 cctttgctgc tattgccgtg ataacacatc accatcaccg gagtgtcaaa gtcgttatca
    69901 cgcataaacg cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc atgccccata
    69961 gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg caacttctgg
    70021 tgcgcgtcgg caacgttaat acattcgaac tgatccatgc gtctctcttt ctttacaaac
    70081 aagtgggcaa atttaccgca cagtttacgt cgaagcggca gataaacgcc ataatgttat
    70141 acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt gctaatatgt
    70201 tcgataacga acatttatga gctttaacga aagtgaatga gggcagcatg gaaaccaaag
    70261 atctgattgt gataggtggc ggcatcaatg gtgctggtat cgcggcagac gccgctggac
    70321 gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca
    70381 gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc cgcctggtca
    70441 gcgaggcgct ggctgaacgt gaagtgctgc tgaaaatggc tccgcatatc gccttcccga
    70501 tgcgttttcg cctgccacat cgtccgcatc tgcgcccggc gtggatgatt cggattggtc
    70561 tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt
    70621 ttggcgcaaa ctcagtgtta aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt
    70681 gggtagacga cgcccgtctg gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg
    70741 aagtgcttac tcggactcgc gccacctctg ctcgccgcga aaacggcctg tggattgtgg
    70801 aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc ctggttaacg
    70861 ccaccggccc gtgggtgaaa cagttcttcg acgaggggat gcatctgcct tcgccttatg
    70921 gcattcgcct gatcaaaggc agccatattg tggtgccgcg tgtgcatacc cagaagcagg
    70981 cctacattct gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg atggacgaat
    71041 tttccatcat cggcaccacc gacgtagagt ataaaggcga tccgaaagcg gtgaagattg
    71101 aagagagtga aatcaactat ctgctgaaag tgtataacgc gcactttaaa aaacagctta
    71161 gccgtgacga tatcgtctgg acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg
    71221 actcgccgca ggctattacc cgtgattaca cccttgatat ccatgatgaa aacggcaaag
    71281 caccgctgct gtcggtattc ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg
    71341 cgctggaaaa actgacgccg tattatcagg gtattggccc ggcgtggacg aaagagagcg
    71401 tgctgccggg tggtgccatt gaaggcgacc gcgacgatta cgcagctcgt ctgcgccgtc
    71461 gctatccatt cctgactgaa tcgctggcgc gtcattacgc acgcacttac ggcagcaaca
    71521 gcgagctgct gctcggcaat gcgggggcga taagcgacct cggggaggat ttcggtcatg
    71581 agttctacga agcggaactg aaatacctgg tggatcacga atgggtccgc cgcaccgacg
    71641 atgccctgtg gcgtcgcaca aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg
    71701 tgagtcagtg gctggtggaa tatacgcagc agaagttgtc gctggcgtcg taaggatgat
    71761 gcccggttga agtgcaaccg ggctatttgg attccttaga ctattcattg taaataattg
    71821 aataacggcc cctgagctaa aatcgccaaa cttaagcacg ttacccaatc gccccacttc
    71881 ttcctctggt ttctattttt tctgcaccaa tggcaataat atcccaatgc aaaaccaagc
    71941 tgtgcactaa tcgggccgac aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc
    72001 aggggaggcc agacaaacgt cagaaagaaa tttcggatat tttccagcaa cgctgatttc
    72061 tctggagaaa tataaagaat gaaattcgag agaattaacc agaaatagac cggcgaaaag
    72121 accagccagc aatgcagtct gttttttaat tcaggaggcg ttctgcggga gaattgccaa
    72181 ccgatgccag ccattacact gaaaaaaagt gcatattcaa ccattaactc aagttgtaat
    72241 tcctgaagag aaatacgcac aaccaggagc ggaaaggaga atattatttc tgccagaaaa
    72301 gttaacgtga atataagtac ggctaagaca ataaccgtcg gccataccaa taatggcacg
    72361 ataacaaaac cgagaattac agggggaata taaagccgga atttaatcat ttttattttc
    72421 ctggtgattc ggatgatgcg tcatacatcg cgcataaaca ctcatcaatt atccagtcgt
    72481 ctacccagac ctgatgctgt gtgccctgtt ctggctgctt tggtgggttc tcaatcgcct
    72541 gccgcgcgcg ttctttcaag atggattgat tgttgaaggc ccaggcattg atttgggtgg
    72601 taatgacgtt atcgatatcg tcctggttat ctctgatata atcctcacaa tatctgttca
    72661 ataaacaggc aagagaaacg gtcgaccaaa agccgggaac tgaattaatg gcatccagca
    72721 atggcgcaag agcgatatcg gcacggaaaa acatttccag catatactgt aattcgacag
    72781 tttcatcgcc ggatacccaa tcagtcatat actggcaggc aaatcgttgc aaaattttcc
    72841 attcatcctc acgccaattt tctttactgg caaggttgat acggcgcaga gaaaaaacta
    72901 cgtgaatatt gtcaaaatgc ccctgtgcaa caaattccag taaacgcggc agaaaatagc
    72961 ggacctcatc gctattttgc ggatcaggcc ctgggctact attccaggca ttgattaagg
    73021 aatacgggat cgctcgtaac gatgtactcc gtaatgcctt ttgttcctgc tgactcaggc
    73081 aatattcaca acaaaccaca aatttctgcg gcacccgata acgacgaaag gtgtgataaa
    73141 tctgctcaat taatgcctgc atggcggggc tgggttgcat agttaccccc tcgcccgctg
    73201 atcgcgtgta aacggttgat agacaaaacg agcattcgcc gcgttcaggc gaataagggc
    73261 gctacgcgaa agggaaaagc tggggcgaat gcaactattg cagtctcgac agctttcctc
    73321 tgcgcaattt tccactgagt aatgacgcgt gtagagtttc agttcggcct gcttatcgcg
    73381 aaatccgcgt aaactgttat tgttatgaaa tttccaggca tctttaaaga cctgaaagat
    73441 atagacatcc tcaatccagg agtcacattt tttaagcggg cgctttgcta ttatcacccg
    73501 ctcatccgga tttagcatcg ccgtaaattg tacggtgacg tcatttgtcg tgttcaggtc
    73561 acattgaaca tctggtacat cactgatata ggggtcaaat ccaaaatcag caccacaggc
    73621 acaaagacgg ctaatggtgg cggtatcgag ggaaaatccc ggccatacat tgctggacgt
    73681 taagccaata tttaatgtga cattatatcg aagcaatccc tcaagaccgt cagcaccgac
    73741 gaacttttcc ccttcatcca gggcctggtt aatgagttgc tgaacaatgc cttcatccca
    73801 gtatgccgat ttgcagttgg caatcgtaat atccacgcct gtgaagggat cagtaccggc
    73861 aaggcttgca aataatgtat taactaattc catttttatt atccagcttt aaggtagcgt
    73921 cttatacatc gggacataac ctacatcttc caccaaactc tgtccctgcg gcgttaaaaa
    73981 ccactccaac aatttttgtg tctctgacgt cgtattttcc cgcgttacca taaaggcatc
    74041 gacgatatag ggatatttgc cgttgcgaat gttttcggca gtcggtgcaa taccgttaat
    74101 cgctagcagt tttatatttt tatcgtcgtt catttgtgtt gcgtaataac ggaaggtgta
    74161 acctattgcg ttgtttgtat tacggtattc ggcaacgact ttaatcattc cctccatcat
    74221 acttgccact tctgtttcct gcggcgagat catgcggaca tttttcatga cctgggattg
    74281 catcaccgtc tggctaccag agtcttccgg gcgttgccag gtctggatct cctgatcgtt
    74341 accgccaacc gtgcgccagt tggtaattgc accactgaag atgtcacgca cttgttgttc
    74401 ggtcagggaa ttaaccgggt tatctgcatt gacgatgaaa acaaacgctt cacgggcaaa
    74461 aggcgtgtaa atcaaagtga cgcccgattc ctccgcgcgt tttttctgcc caccggaagg
    74521 ttgagccaca aagataatat cggcattacc tttaacaatc ttgttatatg cttcgggagt
    74581 acgagagttc gccagatatt cccattcgtg aaaatcttcc ggcaaaacgc ttagtgcata
    74641 aaaggcagag gcataaatgg ggtacgccgc cgttgcgcca tcaagtcgcg gccagttttg
    74701 cgtgaactga atttgcggtt taccacgcag tggtgtcagt tgattattga gtttatcggg
    74761 tcgccaggcc caggtatcaa tattttcacg cacaccgacg ccgggattaa gctgtttata
    74821 taaataagcc tggtaaccgg cgacaaaacc aagcatgaga aggataaaca agagagaatt
    74881 acggcaacgt aacccggacg tgcccgttaa aggatatctg gtcggccagc cgtaccccag
    74941 cgaaaaactg atatgcgcgc caacaggtat aatgactaac caccagtact caccaccgac
    75001 taatgaggct aacaaaacgc ccagccaggg gagcataaga tcagccaacc aacccgctcc
    75061 catctggtga aattgataat ctgccagatg cagcgcgatt atccagaccc ccagggttaa
    75121 taatgcgggt actaaaaaaa gacattgtcg ccagacggct atttctcttt tttctggtgc
    75181 actatcgtcc gcccacactc gccccgagaa aaatagcatt aatgcgccca gcgccagcat
    75241 aaaaatgttg agcagaaggt aaattgttgg cgtgataaag atattacaaa cttcaataat
    75301 accggcaagt ccgacaccca gcatggcaat aaccgccgcc aaaaactgcg ccagaatggg
    75361 gatgccgaaa aaagtcatta ccagcgaggt caaaatccat tttctgtttt gcattattct
    75421 ttccattctt tctgaatggc gaaattatac tccccgagtc cccttgcccc ttctggacac
    75481 ttttccgaaa tgatggcgaa aaaaacggga ccctttggcc ccgttctatt tattagtgaa
    75541 attacaatct caccggatca atatgccaga tatgatcggc gtactctttg atagtacggt
    75601 cagaagagaa gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct
    75661 gaagctcgta gagttcatcg actttatcct gacaatcgac atagctgcga taatccgcca
    75721 gtacctggta gtgatcgccg aagttgatca gcgaatcaac cagatcgcga tagcgacccg
    75781 gatcttccgg actgaataca ccgctgccga tttgcgtcag cacctgatgc agctcctcat
    75841 ctttctcgta gtattcacgc ggtttgtagc cctgacgacg cagttcttcc acttcttccg
    75901 ctgtgttacc aaagataaag atattgtcag caccgacatg atccagcatc tcgacattcg
    75961 caccgtccaa cgtaccgata gtcagcgcac cgttaagcgc aaacttcatg ttactggtgc
    76021 cggaagcttc cgtccccgcc agcgaaatct gttcagacag atcggccgcc ggaatgatca
    76081 actgcgccag gctaacgctg tagttcggga tgaacacgac tttcagcttg tcgccaatct
    76141 gcggatcgtt gttgatcact ttcgctacgt cattgatcaa atgaataatg tgcttcgcca
    76201 tgtaataggc cgaagccgcc ttaccgccaa aaatattcac gcgcggtacc cacttcgcat
    76261 ccgggtcggc cttgatgcgg ttatagcggg taatcacatg caacacattc atcaattgac
    76321 gtttgtattc gtgaatacgt ttgatttgta catcgaacaa cgcctttgga ttcaccacca
    76381 cattcagctg ctgggcgata tactctgcca gacgcttttt gttctccagc ttcgcctgat
    76441 gcacagcgtg attaaccatt gggaaatcac agtgttgttg cagctcatta agcaggctaa
    76501 ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac ggctgaaagc gatgggttcg
    76561 ctaccgctag ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa cgacccggga
    76621 agattttcgc aaagtcggca aacaacgatt gcaccatcag attagagtgc agttccgata
    76681 caccgttaac tttgtggctc acaacaaccg ccagccaggc catacgcaca cgacgaccgt
    76741 tggattcatc aatgatcgac gcccgtccca gcagatcggt atcgttcgga tactgttcct
    76801 gcaaggtttt caggaaatag tcgttgattt caaagatgat ctgcaggtga cgcggcagaa
    76861 ttttacccag catatcaacc ggccaggttt ccagcgcctc gctcatcagc gtgtggttag
    76921 tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc ccagctaaat tggtgctcat
    76981 cgatcagcag acgcatcatc tcaggaatcg acagtaccgg atgggtatca ttgagatgaa
    77041 tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa
    77101 tgtcctgaat ggtcgaggaa accaggaagt attcctgacg caggcgcagc tcacgcccgg
    77161 agtaggtgga gtcatccgga tacagtacgc gagatacgtt ctcggagtgg tttttatctt
    77221 ccactgccgc gaagtagtca ccctggttga atttaccgag gttaatttcg ctactggctt
    77281 gggcactcca caaacgcaac gtgttggtcg cgtcggtatc gtaaccaggg attatctgat
    77341 cgtaagcgac tcccagaatc tcttcggttt caatccagcg cgttttttta ccttcctgct
    77401 gaatgcgacc accaaaacgg actttatagc gcgtgttgtg gcgtttgaat tcccacgggt
    77461 taccgtattc cagccagtag tctggcgact ctttctggct accgttaacg atgttctgct
    77521 tgaacatacc gtagtcatag cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca
    77581 gagaatcaag gaagcaagcc gccagacgtc ccaggccacc gttaccgagg cctgggtcat
    77641 tttcttcatc aatcagctct tcgagattta accccatcgc ttccagtgcg ccctgtacat
    77701 cttcgtaaat tcctagcgac aacatggcgt tggagagcgt acggccaatc aaaaactcca
    77761 tcgacaggta gtaaacctga cgagtttctt gcgacaactg ggcacggttt gaacgtaacc
    77821 agcgctccac gagacgatcg cgcacagcaa ataacgttgc gttcagccat tcatgtttat
    77881 tggcgacgac cgggtccttt ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa
    77941 gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg agcattcata taggcgtttc
    78001 ctgaaaacta tttcaagcga tagtaaagct cacggtacga cttcgccgcg acctgccagc
    78061 taaaatccat tgccatagcc tgacgttgca caaaccgcca cagtgaagga cgggaccaca
    78121 gtacaaaagc acgccgaata gcccgtaaca gcgaccaggc attactatct tcaaagacaa
    78181 acccactggc gacgccatct gcaaggttct caagagaaca gtcagaaacc gtatcagcaa
    78241 gcccaccggt gcgccgcact aacggcagcg taccgtactt caatccataa agttgcgtta
    78301 agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc cgcgccgccc ataatgcgat
    78361 gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg accggggtat tccgctgccg
    78421 ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc
    78481 cctgctccag aagacccggt aaggcttcca gcaccaggtc gagacctttc tggctggtca
    78541 gacggctcac cactgcaaaa agcggcactt tatcgtcaac cttaagcccc attgcgattt
    78601 gtaactggcg cttattttcc gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca
    78661 acagtaagtc cgtctctgga ctccagattt tctcgtccac gccgttcagt acgccggaaa
    78721 gacgcccttc acggtgacgc tgttgcaaca gaccttccat accgtaggca aactgcggtt
    78781 cggtgatctc gcgagcgtag gttggactga ccgccgtaat gtgatcggca tagtacagac
    78841 cggccttcag gaaagagatt tgtccgttga attccagccc atgaatatta aagaatgacc
    78901 atggcaattg gatgtcattc atgtgatgtg cataaaacat gccttgatag gccaagttgt
    78961 gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc
    79021 ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca gaatgggtca agcccgctgg
    79081 ccatttctgc cccaacccac cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa
    79141 ataagttggt atcgtgatac gggcttcccg gacgatcata gagatgcggc gcgtcaatca
    79201 ggtatatgcc aaccccgttg taatgaccga acaacagcgt gatatgtccg gcgaaggtat
    79261 cacgacggga tactacctgc gcatcggtca cgccacggcg aatatcggga aatgcaggca
    79321 acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat
    79381 cagccagacc gccggtttta agcagcggga acatctctga acatacatgt aaaacctgca
    79441 ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg cgcgttacca gcacgatgcc
    79501 ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc
    79561 ttccggaata acacaagcac gatcgatgac gcagcggcgc agacggcacg agcgacctac
    79621 ccatacttcc ggtaacaata cggcggaatc aatgttgcag aatgaattca cgcgaacgcg
    79681 cgagaacaga acggactgca ccaccaccga accggagatc acacaaccgc cggataccag
    79741 tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa
    79801 tgattcattg taggtgcgaa ttggccaatt gcgatcgtac atatccagtt ccggcaccac
    79861 agaggccaga tcgagattcg ctttccagta agcttccagc gtacccacat cgcgccagta
    79921 cggctcggca tccgggtcgg attgtacgca agagagcggg aacgggtgcg cataggccag
    79981 accggcttcg gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg agttctcatc
    80041 acgatcgtct tcttccagca gttcatacag atagtcggcg tcaaagacgt agatacccat
    80101 actcgccaga gatttgctcg gatcgttcgg cattgacggt gggttagcag gtttttcaac
    80161 gaattcgata attttatcgt tctcatcaac cgccataacg ccaaatgcgg aggcttcttc
    80221 aatcggtact ggcatacaag caacggtgca acgtgcgcct ttttcgacgt gatcgataag
    80281 catacgcgag tagtcttgct tgtagatatg gtcgcccgcc aggatcacca cgtattccgc
    80341 tttataacgg cggataatgt cgaggttttg ggtgaccgca tctgcggtgc cgcgatacca
    80401 gttttcccct ttcattctct gctgtgctgg cagcagatcg acaaactcgt tcatttcttc
    80461 attgaagaat gaccagccgc gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt
    80521 gatcacgccc atacgacgga tcccggagtt gatgcagtta gacagcgcaa agtcgataat
    80581 gcggaactta ccgccgaagt gtacggccgg ttttgctcgc ttattggtta aatccttcag
    80641 gcgggtacca cgtcctcccg ccagtatcag ggcaacagat ttcaatggca gctggcgcgc
    80701 caacattaag tgatcgttct tctctaaact aaccatgact aactcctttt ttatcatctc
    80761 tggaacacac acaatccgtg tgcaggtccc tgccagacag ccgtaatcac tgggttatcc
    80821 tctccagcga atgggggaat ggcgtgccac tccccagcag gtaaaacaat ctctgttacc
    80881 tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga gcagaatttg cagctgtttc
    80941 ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat atcgatttag ccaacggaca
    81001 ttgccgtcgc cttcttccca ccagcgattc tccaccaaag cgggaatgcg cttgcgcaga
    81061 tggattaacg cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca gtccaaccag
    81121 gttaattggt tatcctgaca gtaggcattg ttattgccat gctggctgtg accatgttcg
    81181 tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa cagggcgtga
    81241 atgctgtcgc gccgccgttc aaccaggtca agagaaccgc ctaacccttc tttaccatga
    81301 ttgttactgt aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt
    81361 ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta
    81421 atcgcggcac tcggcagacg accattacgt ttaaaaacat cgctggaggc agcaaaacgc
    81481 ccggcaaacg cccccagagg caaatcataa tgtagccaga aacgacgggc agcatcgcgg
    81541 aaatgatcgt tccactcggc aaacagcggc gggaaatttc ccacctgata accaccagga
    81601 gcgatatccc acggttcagc aattaacttc acctgcgaga gcaccgggca gttctggata
    81661 gcggtaaaca acggcgcatc ctgacggaac tctggcgtac ggcccatgac tgccgccaga
    81721 tcaaagcgga aaccatcgac gtggcaggtt tctacccaat aacgcaggca ggcgctggca
    81781 taatccacca ccgccggatg actcaaattg agcgtgttgc cgcaaccggt ccagttgtga
    81841 taatcgccgt cttctcttat ccaataatag ctacggttat cgatcccacg cagcgagaat
    81901 aacgggccgt cgaggtccag ttccgcacta tggttgagca cgatatcaag aatgacttcg
    81961 atacccgctt tatgcagtgc tttgattgca tcgcgaaact catccagcgc cgtttctggc
    82021 gagcaggcat acgccggatg cagcgcaaac atcgccaccg ggttgtaacc ccagtagtta
    82081 cttagcccca tgcgttgcag acgtggttca ctggcaaact gcgccactgg cagcagttcc
    82141 agcgcggtaa tgcccaattg tttcaaatag ttgatcatca ccggatgccc gagggcttta
    82201 taagtgccac ggatctcgac cgggatctcc gggtgcaagt acgttaatcc tttgacatgg
    82261 gcttcataaa tgatggtgct gccccacggc gtgcgcggcg gggcatcatc ttcccagtca
    82321 tagtgatcaa ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt
    82381 tcattatgac cggcgtgcag cagcgggtta tctttaaact ccccgtcaat ttgccgcgcg
    82441 caaggatcaa tcaacaactt cgccgggtta aagcgatgcc cctcggcggg ttgccagggg
    82501 ccatgaacgc gataaccata acgcaaaccc gggcgcgcat ccggcagata accgtgccaa
    82561 atgtcgccac tgtgccctgg caagtcatag cgatgttcct ggccattggc gtcaaagaca
    82621 cacagttcta cccgctcggc atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg
    82681 taatgcgcgc cgaggggagc gggtttgcca atggcgagtt gtgtcattct gcctcccgaa
    82741 ccagccagat agtggccagc ggtggtagcg tcaggcttag tgaatgctga cgaccgtggc
    82801 tggcaatctc atcgctgtgt accgtgccgc cattgcctgc attactgccg tgatagtgca
    82861 tggaatcggt attgaggatt tcacgccatt tgcccggctg gtttatgccg aagcgataat
    82921 catgacgcgg taccggcgta aagttactgg caacgatgat ttcgttaccc tctttatcgc
    82981 gacgcacaaa gatcagcacc gagcgttctt tgtcatccac caccagccat tcaaagccgt
    83041 acgggtcaaa atccagttca tgcattgctt tatggtggcg gtaggtgagg ttcagatcgc
    83101 gcaccagacg ctggacaccg tggtgccagt tatcgccgcc ttccaacaga tgccagtcga
    83161 ggctggcgtc atggttccac tcgcggccct gggcaaattc gttacccatg aacagtagtt
    83221 tcttgcccgg gaatgcccac atccagccat agtaggcgcg caggttcgcg aatttctgcc
    83281 atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc gtggaccact tcatcatgcg
    83341 acaacggcag gacgaagttt tcagtgtagt tgtagagaat cccgaaggtc agtttatcgt
    83401 gatgatactg acgataaacc gggtcgagct tcatgtagtc cagggtgtca tgcatccagc
    83461 cgaggttcca cttgtaccag aagcccagac cgcccatatc ctgcggacga gaaacgccag
    83521 ggaaatcggt agactcctca gccattgtca ccgcaccgga aacctgctca ccaagaatac
    83581 ggttggtatt acgcaagaat tcaatcgctt caagattctc gcgcccgcca aattcgttcg
    83641 ggatccactc cccctcttta cggctgtagt cgcgataaat cattgacgcc accgcatcga
    83701 cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag cgcgttaccg acgaggaagt
    83761 tactgacttc acggcgacca tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt
    83821 cacgcggatc gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat
    83881 cagtcgggaa gtggcctggc acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg
    83941 catcaatgaa ataacggaag tcgtcgcgag taccaaaacg gcgggttggt gcatacaggc
    84001 cggttggctg ataaccccaa ctgccatcga agggatgctc gttaatgggc agtagttcga
    84061 ggtgggtaaa gcccatccat ttagcataag gcaccagttg atcggccagc tcgcggtagc
    84121 tcaaccagaa attgttgtcg gtgtggcgac gccaggaacc caggtgaact tcataaatag
    84181 agattggcgc atcaaactga ttcgcttttt tgcgctcttc agtctgtaca accttttccg
    84241 gcagcccgca aataagagac gcggtttccg ggcgcatttg cgcttcgaag gcataagggt
    84301 cggacttcag acgcaagttg ccattggcat caatcatctc gtatttatag agctgaccgt
    84361 tatgcgcccc agggataaac agttcccaga tgccgctctc tttacgcagg cgcatcgggt
    84421 gacggcgacc gtcccagtag ttaaattgcc caaccaccga gacccgacgg gcgtttggag
    84481 cccagacaga gaaacgcgta cctgtgacgc catccatagt atctgcatgc gcgcctaagg
    84541 tttcatacgg gcgcaggtga gtaccttcag ataatagcca ggcatccatt tcctggatta
    84601 gcggaccaaa acggtaagga tcatcaatca ggttttgctg accatgccag acaacagcca
    84661 actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt
    84721 cgagacactc cagttttgcg agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg
    84781 tagcgtcggg taaaagggca cggacttcca gtcccgcggt ggttttatgc attcccagta
    84841 cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac
    84901 gatcggacat gcttgtcttc ctgttttatt gtgtcacccc atccaattgg atttttgact
    84961 tctggttgtg acattttttt gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc
    85021 gtcctcactt caggtaaggc tgtgaatact catgtattca gccaccctta aagaatagcc
    85081 aatgctctat ttaactcccg gtaaatcatg aaacatctgc gcttactcct gtattacgca
    85141 ctaacagggg cggcatcgcg ccccagattt aatgaataaa gattacgcca gttgacgaag
    85201 catccgacgc agcggctccg cggcccccca cagcagctgg tcgcccacgg taaaggctga
    85261 caggaactct ggtcccatat tcagcttacg caggcggcct accggcgtgg tcagcgtgcc
    85321 ggtaacggca gctggggtta gctcacgcat agtgatttcc cgatcgttcg gaacgacttt
    85381 cgcccacgga ttgtgcgcag ccagcagttc ttccacggtc ggaatagaca catctttttt
    85441 caatttaata gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca cacataaacc
    85501 atctaccgga attacggaag atgtgttgag gatcttgttg gtttccgcct gccctttcca
    85561 ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc cacggaatca ggctacccgc
    85621 cagcggcacg ccaaagttat ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt
    85681 gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt tcatctgcca catggccata
    85741 cagatggccc atctgggtta ataactcacg catatgtcgc gcaccaccgc cggaagcggc
    85801 ctggtaggtt gcaacggaca cccaatcaac aagatcattg gcgaataaac cacccaacga
    85861 catcaacatc aggcttacgg tacagttacc gccaacaaaa gtcctgatgc cattatttaa
    85921 tccgtcggta atgacgtcct gattgacggg gtcaagaatg atgatggcgt catctttcat
    85981 gcgcagagac gatgctgcgt caatccagta accttgccat ccgctttcac gaagctttgg
    86041 atagatttcg ttggtataat cgccgccctg acaggtcaca atgatatcga gggcctttag
    86101 cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc
    86161 ctggccaagc tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc
    86221 aaccatgcgt tgcatgagaa cggagccgac cataccgcgc cagccgataa aaccaacatt
    86281 tttcataagc gtttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt
    86341 ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag
    86401 cgaagccatc agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat
    86461 tatcagggaa tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt
    86521 atggatccgc tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa
    86581 agacggcggg caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc
    86641 ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc gagcagaaac cgtctccatt
    86701 tctggcggca tcattctgtt tctaatcgcc attaagatga ttttccccag cgcttcagga
    86761 aatagcagcg ggcttccggc aggtgaagag ccatttatcg tgccgttggc aattccgtta
    86821 gtcgccgggc cgactattct cgccacgctg atgttgctgt ctcaccagta cccaaatcag
    86881 atgggacatc tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta
    86941 cagtcttcgc tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg
    87001 atgggattga ttctggtgat gatggcaacc cagatgttcc tcgacggcat tagaatgtgg
    87061 atgaaggggt aaggttcaaa caggtgctgc acacaaccag acgcggcacc tgttttatta
    87121 cctgaactga taatgacctt cgcccaatcg gataaccctt ccctcttctt ctgcgcggtt
    87181 cagccacaca ctaatttgtt cgatggttaa accagtacaa gacgacagcc tttcaatact
    87241 tatcgattcc tttgcaaaga tcgccagttc cgccacaaag agttgataaa aatcgacagg
    87301 cgtatgcgcg ggcattactg cgtaatttgc gtcattttca tgcagcgaaa acagttctgg
    87361 ttgagcgtta cggagtgctc tgtcatacat cagcccagga acgtctgaca gtctgatcgg
    87421 cacattctca gcctgttcag cactccatga cgccccattt tctactaatc gtgaattggc
    87481 tgattgcata tcctgatttg gtttaacctg cacaggcagc cactgatgtt ttaatgcctc
    87541 tatcgccccg gttatcgttc caccggtcat tcccgcacgt acaaccattg cgctttcagc
    87601 aaggcaataa atatatttat ttcgcgccat cgcattgccg acggtgaaac gcacctctgg
    87661 gtaaaacggc gaaatcaaca ccaggttgcc tgctataagc ccttcacgcc atttcactaa
    87721 cgtactcgtt tttaacaagc tatcagctaa tacgccaacg gcagttcccc cggcctccag
    87781 tgccgacgcc attgcacatt catcaatacc tcgcgcacca ccagagataa cgcaaatccc
    87841 ctgttgggcc agtttcgcgg ccagttgttg ggtatagcgc aaatcgtcag tcggagcatc
    87901 tcgcgagcca acaatcgcca taccttccgc ttgcagtaat gctttattcc cgcagccaaa
    87961 taaaacggga ggtgcatccg ttcgcaatcg gtttttcaag cgaacgggat aatcagcatc
    88021 tcctcgggtt atgatccata aaccggcgcg ttgccactta tctaccgcca acgccagact
    88081 atgaccgcgc gccagcaagc caagaatacg ctcctgagaa atacgcggat cgctccagcc
    88141 tgtaagtttc tcttgcggtt gcggcaccag aagctcggcg ggactgatac gttggtgctt
    88201 cagccataat gcaaacttcc cccattcact attactaaga ggtttatgct cactcgccgc
    88261 cgcgcgagaa aaatcgctgg ttagcaggag agtcgcttgt gcattggctg aaagattcat
    88321 caatttttta ccgaggtaga agcaagggca agaggataaa ccgtcgggca acctgcctgg
    88381 cgtaacagtg ctgtcagaac tgtcagcgtc catgcagaat ccacgatatc gtcaaccagc
    88441 aacgccggac ctggcatcaa aggaggggta atcacaaacg ccccgtcgag attttgacac
    88501 tggtgaaaac ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca
    88561 tcaataaaag gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg
    88621 aggtgcctta atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt
    88681 tggcgaacca tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc
    88741 tcctgtgcta cctgctttcc ccatccggaa tctccccaac gagaaaggac tcgccccgta
    88801 gatccttgta aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat
    88861 gctacctgtt tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca
    88921 tggagtaagc cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat
    88981 ttaccacaac gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac
    89041 tccttggtcg tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa
    89101 tggttaattc gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg
    89161 gtatacacca caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc
    89221 ccataacgga gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta
    89281 agtacgttga gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat
    89341 ttatgaatag cgcggtcttc accaccacaa agcaatatgc caacagcgga atctatagca
    89401 cgcccggcac gccctacctg ttggtagtaa ccgacaatag accccggcat ctggtaatga
    89461 ataacaaacc ccaaatccgg tttgtcaaag cccattccca gcgccgtcgt cgcgaccaga
    89521 actttaattt tatttgccag caacgcctgc tccagatatt ccctggcagt attagaatct
    89581 tccgcgcctt cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt
    89641 aaccactcgg cgacaaggtc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg
    89701 gaaaactgag ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact
    89761 aaggcatcaa gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc
    89821 aattgctgac ggatatcctc aacgacacgg ttattcgctg tcgcggttgt acccagaact
    89881 ggggtattcg caggcagttg gcgcaaaata tctaatatac gtcggtaatc cgggcgaaaa
    89941 tcgtggcccc aatcagagat gcagtgcgcc tcgtcgacca caagcaagcc aatacgatcg
    90001 gctataggat ataaaaccgt ttcgataaag tcttggtttg ccaaacgttc cggggaaacc
    90061 agcaagcaat cgacctcacc ttgcagcaac ttatcgctaa tacgctgcca ttcttccctg
    90121 tttgtagaat tcaacgtttc agccgtaata cctaaccgtt cagcggcggc aacctggtta
    90181 cgcatcaggg caagcaaagg agagatgata attgtcgggc cagcaccgcg atcgcggaag
    90241 attttactgg cgatgaagta cacggcactt ttcccccagc cagtgcgttg gacaaccagc
    90301 agttttcgtc gttggttcac caccgcgtct atggcttccc attgcccttc cctgaaagta
    90361 gcggtggtat ttcctaacat ccgctgtaaa agcaattcag ctgcgtgctt ttccatcttt
    90421 atctcctgcg atctttaggc agtgattaga acggattatt agcaggtaaa tttgcgagag
    90481 ggatcaaaaa gatgaagaga ggagataaga gaaatgaatt actgcgagca agctcgcagt
    90541 aatttgtcgc agtaattaac tcaacagctg gaacgcaatc atcccaacga tggcaccgac
    90601 agtgccgagg atggtttcca tcatggtcca ggttttcagc gtttcggctt cggtcgcgcc
    90661 ggtaaattta ccgaacaacc agaaaccggc gtcgttaacg tggctgacaa caatcgaacc
    90721 accagcgata caaatcgaca gcgccgccat ttgcgcaccg gagtagttca gttgttcaat
    90781 aatcggcatc accagtccta ccgccgttaa acaggctacg gtggcagaac cctgaatgat
    90841 gcgcactgca gctgccagca cgaagcaggt gatagcaatc ggcaggccca tgccggttaa
    90901 cgcttcgccc agtgccggac ctacgccaga gtcaaccagc acctgtttga acacgccgcc
    90961 tgcaccaatc accagcagaa tgatccccgc cggttgcagc gcgtgaccgc aaatctccat
    91021 cactttgtct tttggcatgc cctgacgcat tgccaggcca taaatcgcta ccagacaagc
    91081 aaccagaatc gcggtaaacg gatgaccaat aaactcgaac cattcgtaag cggtagagcc
    91141 ttctggcaca aaacgcgcgg caatggtttt cagccccacc agcaccagcg gcaacaggat
    91201 cagcgacagg ctgaatccga aagatggcat tttgccttcg ccgagatgcg gttcgctgat
    91261 gtcgtcagga atatgcaact ccacgtagcg gctgatgaaa ttaccccaca gcggcccggc
    91321 aataatcatt cccggaattg ccgcacacag gccaatcagg atcatccagc caaaatcggc
    91381 gttcatctgc gatgccagca gcattggcgc tggccctggc accaggaacg ccgccgctgc
    91441 cgccacgcct gcgaataatg ggattaccag cttcaccagg ttcgtaccgg tgtggcgcgc
    91501 cattgagaaa gcaacgctaa tcagcagaac aatcgccact tcaaagaaca gcggtagcgc
    91561 acagaccagc cccgcaaggc cgatggcata atgcgcgcgg ctgtgaccga aggatttaag
    91621 cattttgacg gcaatctgat cgactgcgcc ggtttcatgt aagatcttgc caaacatagc
    91681 tcccagggcg acaaccaccg ccaggaagcc gagggtgcct cccatccctt tttccatcgt
    91741 cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca gcccccatgg acaccaccat
    91801 caaagccagg aaagcgtgca tacgcgcctt catgactaaa aacagcagca gtaaaacaga
    91861 ccctactgct gttaaaacaa gcgttaatgt agtcactact tatttgcctt ttttaataac
    91921 ctcaatggtg cttgccacaa caccttccag cggttgatcg atatccacca ccagtacatc
    91981 agtttcgtcc gcacccggct cctgcagcgt ttcaaactgc gtcaccaaca tttgggtttt
    92041 aaagaaatgg cctttgcgcg ctttcaggcg gctttcaatc acgtcaaaat cgcctttcag
    92101 gtagatgaaa gagagattcg gattaccttc acgcagcaag tcgcgatagt gttttttcaa
    92161 tgcagaacag acgatcagtg acactttatt agtgcgctgc atagcaaacg cggcgtcgtt
    92221 cagcgcctgc aaccacggtt tgcgatcgtc gtcattcagc ggttcgccag acgccatttt
    92281 ttcgatattg cgccgtggat ggaggaaatc gccatcaaga aacgcggcat gaagttgatg
    92341 cgccacttca ctggcgaccg cagatttgcc gctgcctgag acgcccatca agacgtaaat
    92401 gtggtgatca tggttagtcg tgctcaaagt ggtgccccca caatacaaga attaaaaatg
    92461 ttacgggtaa ctgttatcgg taacattgtc cggccggaca atatcagaag caatatccat
    92521 ccgtgcctta agtgtataag tgtgagctac ttcaaatttg tgggcttaaa tagatccgcc
    92581 cggtgacaag gtgaaaccta aatctaacat tttcggtgtc acagattcgc cacgaatacg
    92641 cgccagcagg cgttcagcgc caatactgcc catccgctca cgcggcgtca gcacgctcgc
    92701 cagtcgtggc tccatcacct gaccaatgtc atgaccgtgg aaaccggcaa tcgccatatc
    92761 gtcaggaact tttaacccca gacgctgaca ttcaaacgcc gcgccgaccg ccagatcatc
    92821 attagtacag aacacgccat ccagctgcgg atattcccgc cgcgcctggc gaatcagttc
    92881 aataccggaa gagtaagaag aagattgctc aaccatcacg ctatacggca ccaggcctgc
    92941 atccagcatc gcctgttcgt atcccttctg tttgatgata gtacgttcgt cgagacgtgc
    93001 gccgagatag gcaatgtggc gatgcccgcg cgcaataatg gcggtagtca tctggcgtgc
    93061 cgcttcaaag ttatcaaaac cgacggcgat atcgaggcac ggcgactggc tgtccatcaa
    93121 ctccaccacc gggatccccg ccacttcaat catctttaag gtgcgcggcg tgtgggtacg
    93181 ttcggtgagg atcaggccgt cgatattcca ggacagcatg gattcgagac gttcttgctc
    93241 catttccggt ttataaccgt agtgcgccag catcgtctga taaccgtgcg cgtcggtgac
    93301 gctttcgatt ccgcgtaata cttccgcgaa aacctggttg gtgagagaag gtaacaggac
    93361 gccaatcgcc cggctggtag cgttagagag gatatcgggc gcacgattgg gaatatagcc
    93421 cagttcatca agagcggcgg caatcttgcc gcgtagagcg acggaaacct gctccgggtt
    93481 acgcaaaaag cggctgaccg tcattttggt cacgcctaca cggtcagcca catcctgaag
    93541 tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag agatagattc tcttagttta
    93601 acacggacgc atcagaacct tcccggggaa aacagtgctt gtttacaatt attagatata
    93661 cactaaatca attgcatcgc attgtgctaa cgacgcggca aaaccccgga agcgtgcatc
    93721 agcacgcgac cggagcgagc gaggaagcct gcaaaaaggc aggcttcaaa gatgccgttg
    93781 agttttaaac cggcggcaga tcgaacagta acacttcgct gtcgctatcc gcatggatgg
    93841 agattgcctg ctcatcccag attgccagac catcgctggt cgaggctttc acgccgttaa
    93901 tggtgacatt gcctttcacc acctggatcc agacgcggcg ttcagcggca atctgatgca
    93961 ccgactgctc atctttcagc aacgcccaac gatacagttc catatcctga tgcactttca
    94021 gcgaaccatc tcgcgcatcc ggcgagagca ccagctgttt gccctgcacg gcatcgaagc
    94081 gacgctgttc ataacgcggc gtaatgccat tttcttcagg catgatccag atctgataca
    94141 gatgcagacg ctcggtgctg cttgggttgt actctgagtg acgaataccc gtacccgcac
    94201 tcataatctg gaactcaccc gccggaacct gctctttatt gcccatgctg tcctgatgct
    94261 caacagtacc ttccagcacg taggtcaaaa tttccatatc tttatgcggg tgagtgccga
    94321 agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg cagcgcggaa aagcccataa
    94381 agttcggatc gtaatagttg gcaaaagaga aagtatgcca ggagtccagc cagccatgat
    94441 ttgcatgacc acgttcattt gctttgcgta agtagatcat ttgtattcgc cccatgaatg
    94501 atttcgatgg gcttagtgtg gacccgatct gcctgggatg atagagggtg aaaattgacc
    94561 cctctgttca aaaaaattga actattcgag gggccatcaa acttacttag cgagagttac
    94621 tgtggaggga gaggcttgct caaatccgcg ttcaaggatt tccagattcg taagaacttc
    94681 agattccttg acgtaatttg gcgcaccgtg ggtgatggtt tgatacaacg catcataaac
    94741 gcgcccgtaa tcgcccatct ccggcttcat ctcttctctg accgtcacgc cctcgtcatt
    94801 gacatactcc agcacaccga ccgaatcatc cgctgcgaat cccggttcgc ccggcataat
    94861 attagccttc aggctggttt cctgctggtc gataccgtat ttaataaacg aacctttctt
    94921 accgtgaacg ataaatttcg gataatcgat tttcaccaga tggctggttt tgacgatggc
    94981 tttcaggtcg ccataaaaca gttgcgcttc aaaggtgtcg tcaggattgg ctttattacg
    95041 caggctgcgg atgtcataag cgacgtgatc cgggcgaccg aacagagaaa taatctggtc
    95101 catcgtatgc acaccaaggc cataaaacgc gccatcctgc ggcagcccag gtttggtctc
    95161 tgccaccggg cggtaatagt caaaatggct ttccacttca acaatctctc ccaacttgcc
    95221 actttcaatc gcttttttcg ctgtcaggaa gcaggagtca aagcgacgat tctgatacgg
    95281 cgtgacggtc agccctttgc ttttcgccaa cgcaaacagc tccttcgcct gggcaagcgt
    95341 cggggtgaac ggtttttcga ccagcacatt tttcccggct tccagcgcgc gtttcgcgta
    95401 ctcgaaatgg ctgtccgcgt gggtgcagac aacaaccagc ttaacatcgg gatcgtttag
    95461 tacttcgtcg agatcgctgg tgaaatggat atgggaataa atgggagcct gttcttccgg
    95521 cttcgcatgg cgacgaaaaa tatgcgcgac atgccagcta tccttgcggt taagtacata
    95581 cggcagatgg taacgggtgg tgcttttgcc gaagccaata aaggcgcagt tgatgaccat
    95641 gatttcgtcc tttttaaggt ggttatttac accttagcgc aaagcggacg tggttcctac
    95701 tggagtgcgc ataacgaaca caagcactcc cgtggataaa ttgagaacga aagatcaaaa
    95761 aaaagccagc acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt
    95821 caggttctcc gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact
    95881 tgtccaacac tttttgcaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga
    95941 aagggacatt taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga
    96001 aacacgggat tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac
    96061 actacaggtg cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcccgg
    96121 catcaagcaa ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca ccattgacgt
    96181 gcaacagcgc ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actctcgttg
    96241 gaagaaccgc ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg
    96301 gttgcgggta gatcgcattg aactaaccgt gtttgtcgat aacgccccgg caattaaggt
    96361 ctataaaaaa tacggctttg aaattgaagg gactggtaag aagtacgcat tgcgtaatgg
    96421 tgaatatgtc gatgcatatt atatggcgcg ggtgaagtaa gatagtgccc tttttctgag
    96481 atggaaaaag ggtgtcattc aaaatcgaca taccttcctt taaggtattt atttgcccaa
    96541 tacatatatt gagatttaac tcataattac ttcc
//
LOCUS       DAZGCS010000002        86702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGCS010000002 DAZGCS010000000
VERSION     DAZGCS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.002"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            224..850
                     /gene="ydhY"
                     /locus_tag="ACHK01_000082"
     CDS             224..850
                     /gene="ydhY"
                     /locus_tag="ACHK01_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO8095100.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            871..2973
                     /gene="ydhV"
                     /locus_tag="ACHK01_000083"
     CDS             871..2973
                     /gene="ydhV"
                     /locus_tag="ACHK01_000083"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO8095101.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYLLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2986..3624
                     /gene="ydhW"
                     /locus_tag="ACHK01_000084"
     CDS             2986..3624
                     /gene="ydhW"
                     /locus_tag="ACHK01_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO8095102.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3688..4356
                     /gene="ydhX"
                     /locus_tag="ACHK01_000085"
     CDS             3688..4356
                     /gene="ydhX"
                     /locus_tag="ACHK01_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8095103.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4353..5138
                     /gene="phsC"
                     /locus_tag="ACHK01_000086"
     CDS             4353..5138
                     /gene="phsC"
                     /locus_tag="ACHK01_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO8095104.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5142..5954
                     /gene="ydhT"
                     /locus_tag="ACHK01_000087"
     CDS             5142..5954
                     /gene="ydhT"
                     /locus_tag="ACHK01_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO8095105.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5966..7570)
                     /gene="ydhS"
                     /locus_tag="ACHK01_000088"
     CDS             complement(5966..7570)
                     /gene="ydhS"
                     /locus_tag="ACHK01_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO8095106.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7696..8001)
                     /gene="ydhR"
                     /locus_tag="ACHK01_000089"
     CDS             complement(7696..8001)
                     /gene="ydhR"
                     /locus_tag="ACHK01_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO8095107.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8109..8185)
                     /locus_tag="ACHK01_000090"
     tRNA            complement(8109..8185)
                     /locus_tag="ACHK01_000090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8149..8151),aa:Val,seq:gac)
     gene            complement(8190..8266)
                     /locus_tag="ACHK01_000091"
     tRNA            complement(8190..8266)
                     /locus_tag="ACHK01_000091"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8230..8232),aa:Val,seq:gac)
     gene            8574..9830
                     /gene="ydhQ"
                     /locus_tag="ACHK01_000092"
     CDS             8574..9830
                     /gene="ydhQ"
                     /locus_tag="ACHK01_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8095108.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9871..11244)
                     /gene="mdtK"
                     /locus_tag="ACHK01_000093"
     CDS             complement(9871..11244)
                     /gene="mdtK"
                     /locus_tag="ACHK01_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO8095109.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11459..12100
                     /gene="ribE"
                     /locus_tag="ACHK01_000094"
     CDS             11459..12100
                     /gene="ribE"
                     /locus_tag="ACHK01_000094"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO8095110.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12140..13288)
                     /gene="cfa"
                     /locus_tag="ACHK01_000095"
     CDS             complement(12140..13288)
                     /gene="cfa"
                     /locus_tag="ACHK01_000095"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO8095111.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13579..14790)
                     /gene="punC"
                     /locus_tag="ACHK01_000096"
     CDS             complement(13579..14790)
                     /gene="punC"
                     /locus_tag="ACHK01_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO8095112.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14903..15835
                     /gene="punR"
                     /locus_tag="ACHK01_000097"
     CDS             14903..15835
                     /gene="punR"
                     /locus_tag="ACHK01_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO8095113.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMNGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15832..16857)
                     /gene="purR"
                     /locus_tag="ACHK01_000098"
     CDS             complement(15832..16857)
                     /gene="purR"
                     /locus_tag="ACHK01_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO8095114.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17156..17245
                     /gene="cydH"
                     /locus_tag="ACHK01_000099"
                     /gene_synonym="ynhF"
     CDS             17156..17245
                     /gene="cydH"
                     /locus_tag="ACHK01_000099"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO8095115.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17411..18580
                     /gene="ydhP"
                     /locus_tag="ACHK01_000100"
     CDS             17411..18580
                     /gene="ydhP"
                     /locus_tag="ACHK01_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8095116.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18726..19307)
                     /gene="sodB"
                     /locus_tag="ACHK01_000101"
     CDS             complement(18726..19307)
                     /gene="sodB"
                     /locus_tag="ACHK01_000101"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO8095117.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19435..20250)
                     /gene="mepH"
                     /locus_tag="ACHK01_000102"
     CDS             complement(19435..20250)
                     /gene="mepH"
                     /locus_tag="ACHK01_000102"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO8095118.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTTSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20585..20932
                     /gene="grxD"
                     /locus_tag="ACHK01_000103"
     CDS             20585..20932
                     /gene="grxD"
                     /locus_tag="ACHK01_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO8095119.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20983..25599)
                     /gene="lhr"
                     /locus_tag="ACHK01_000104"
     CDS             complement(20983..25599)
                     /gene="lhr"
                     /locus_tag="ACHK01_000104"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO8095120.1"
                     /translation="MADNPDSSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHQARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25692..26339)
                     /gene="rnt"
                     /locus_tag="ACHK01_000105"
     CDS             complement(25692..26339)
                     /gene="rnt"
                     /locus_tag="ACHK01_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO8095121.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26442..26849)
                     /gene="gloA"
                     /locus_tag="ACHK01_000106"
     CDS             complement(26442..26849)
                     /gene="gloA"
                     /locus_tag="ACHK01_000106"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO8095122.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26930..28027)
                     /gene="nemA"
                     /locus_tag="ACHK01_000107"
     CDS             complement(26930..28027)
                     /gene="nemA"
                     /locus_tag="ACHK01_000107"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO8095123.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28064..28663)
                     /gene="nemR"
                     /locus_tag="ACHK01_000108"
     CDS             complement(28064..28663)
                     /gene="nemR"
                     /locus_tag="ACHK01_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO8095124.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28766..29005
                     /gene="ydhL"
                     /locus_tag="ACHK01_000109"
     CDS             28766..29005
                     /gene="ydhL"
                     /locus_tag="ACHK01_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO8095125.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29054..29950
                     /gene="ydhF"
                     /locus_tag="ACHK01_000110"
     CDS             29054..29950
                     /gene="ydhF"
                     /locus_tag="ACHK01_000110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO8095126.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30031..30552
                     /gene="sodC"
                     /locus_tag="ACHK01_000111"
     CDS             30031..30552
                     /gene="sodC"
                     /locus_tag="ACHK01_000111"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO8095127.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30553..32565)
                     /gene="ydhK"
                     /locus_tag="ACHK01_000112"
     CDS             complement(30553..32565)
                     /gene="ydhK"
                     /locus_tag="ACHK01_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8095128.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDVGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKSQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32565..33422)
                     /gene="ydhJ"
                     /locus_tag="ACHK01_000113"
     CDS             complement(32565..33422)
                     /gene="ydhJ"
                     /locus_tag="ACHK01_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8095129.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33425..33661)
                     /gene="ydhI"
                     /locus_tag="ACHK01_000114"
     CDS             complement(33425..33661)
                     /gene="ydhI"
                     /locus_tag="ACHK01_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO8095130.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33862..34296
                     /gene="slyA"
                     /locus_tag="ACHK01_000115"
     CDS             33862..34296
                     /gene="slyA"
                     /locus_tag="ACHK01_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO8095131.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34343..34810)
                     /gene="slyB"
                     /locus_tag="ACHK01_000116"
     CDS             complement(34343..34810)
                     /gene="slyB"
                     /locus_tag="ACHK01_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO8095132.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35084..36193
                     /gene="anmK"
                     /locus_tag="ACHK01_000117"
     CDS             35084..36193
                     /gene="anmK"
                     /locus_tag="ACHK01_000117"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO8095133.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36291..36620
                     /gene="mliC"
                     /locus_tag="ACHK01_000118"
     CDS             36291..36620
                     /gene="mliC"
                     /locus_tag="ACHK01_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO8095134.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36679..37335
                     /gene="pdxH"
                     /locus_tag="ACHK01_000119"
     CDS             36679..37335
                     /gene="pdxH"
                     /locus_tag="ACHK01_000119"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO8095135.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQLENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37464..38738
                     /gene="tyrS"
                     /locus_tag="ACHK01_000120"
     CDS             37464..38738
                     /gene="tyrS"
                     /locus_tag="ACHK01_000120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO8095136.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38797..39660
                     /gene="pdxY"
                     /locus_tag="ACHK01_000121"
     CDS             38797..39660
                     /gene="pdxY"
                     /locus_tag="ACHK01_000121"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO8095137.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39704..40309)
                     /gene="gstA"
                     /locus_tag="ACHK01_000122"
     CDS             complement(39704..40309)
                     /gene="gstA"
                     /locus_tag="ACHK01_000122"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO8095138.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPAVLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40415..41917)
                     /gene="dtpA"
                     /locus_tag="ACHK01_000123"
     CDS             complement(40415..41917)
                     /gene="dtpA"
                     /locus_tag="ACHK01_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO8095139.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42528..43163)
                     /gene="nth"
                     /locus_tag="ACHK01_000124"
     CDS             complement(42528..43163)
                     /gene="nth"
                     /locus_tag="ACHK01_000124"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO8095140.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43163..43858)
                     /gene="rsxE"
                     /locus_tag="ACHK01_000125"
     CDS             complement(43163..43858)
                     /gene="rsxE"
                     /locus_tag="ACHK01_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO8095141.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43862..44482)
                     /gene="rsxG"
                     /locus_tag="ACHK01_000126"
     CDS             complement(43862..44482)
                     /gene="rsxG"
                     /locus_tag="ACHK01_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO8095142.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44486..45544)
                     /gene="rsxD"
                     /locus_tag="ACHK01_000127"
     CDS             complement(44486..45544)
                     /gene="rsxD"
                     /locus_tag="ACHK01_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO8095143.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45545..47767)
                     /gene="rsxC"
                     /locus_tag="ACHK01_000128"
     CDS             complement(45545..47767)
                     /gene="rsxC"
                     /locus_tag="ACHK01_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO8095144.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQSNAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(47760..48338)
                     /gene="rsxB"
                     /locus_tag="ACHK01_000129"
     CDS             complement(47760..48338)
                     /gene="rsxB"
                     /locus_tag="ACHK01_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO8095145.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48338..48919)
                     /gene="rsxA"
                     /locus_tag="ACHK01_000130"
     CDS             complement(48338..48919)
                     /gene="rsxA"
                     /locus_tag="ACHK01_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO8095146.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48996..49436)
                     /gene="ydgK"
                     /locus_tag="ACHK01_000131"
     CDS             complement(48996..49436)
                     /gene="ydgK"
                     /locus_tag="ACHK01_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO8095147.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49522..49737)
                     /gene="ydgT"
                     /locus_tag="ACHK01_000132"
     CDS             complement(49522..49737)
                     /gene="ydgT"
                     /locus_tag="ACHK01_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO8095148.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50010..50135)
                     /gene="blr"
                     /locus_tag="ACHK01_000133"
     CDS             complement(50010..50135)
                     /gene="blr"
                     /locus_tag="ACHK01_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO8095149.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50378..51418
                     /gene="ydgJ"
                     /locus_tag="ACHK01_000134"
     CDS             50378..51418
                     /gene="ydgJ"
                     /locus_tag="ACHK01_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8095150.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51453..52454)
                     /gene="add"
                     /locus_tag="ACHK01_000135"
     CDS             complement(51453..52454)
                     /gene="add"
                     /locus_tag="ACHK01_000135"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO8095151.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52558..53730)
                     /gene="malY"
                     /locus_tag="ACHK01_000136"
     CDS             complement(52558..53730)
                     /gene="malY"
                     /locus_tag="ACHK01_000136"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO8095152.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53740..55332)
                     /gene="malX"
                     /locus_tag="ACHK01_000137"
     CDS             complement(53740..55332)
                     /gene="malX"
                     /locus_tag="ACHK01_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO8095153.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55507..56535
                     /gene="malI"
                     /locus_tag="ACHK01_000138"
     CDS             55507..56535
                     /gene="malI"
                     /locus_tag="ACHK01_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO8095154.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56647..57414
                     /gene="hdhA"
                     /locus_tag="ACHK01_000139"
     CDS             56647..57414
                     /gene="hdhA"
                     /locus_tag="ACHK01_000139"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO8095155.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57643..58233
                     /gene="uidR"
                     /locus_tag="ACHK01_000140"
     CDS             57643..58233
                     /gene="uidR"
                     /locus_tag="ACHK01_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO8095156.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58621..60432
                     /gene="uidA"
                     /locus_tag="ACHK01_000141"
     CDS             58621..60432
                     /gene="uidA"
                     /locus_tag="ACHK01_000141"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO8095157.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60429..61802
                     /gene="uidB"
                     /locus_tag="ACHK01_000142"
     CDS             60429..61802
                     /gene="uidB"
                     /locus_tag="ACHK01_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO8095158.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSFATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61841..63106
                     /gene="uidC"
                     /locus_tag="ACHK01_000143"
     CDS             61841..63106
                     /gene="uidC"
                     /locus_tag="ACHK01_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO8095159.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63151..64659)
                     /gene="ydgA"
                     /locus_tag="ACHK01_000144"
     CDS             complement(63151..64659)
                     /gene="ydgA"
                     /locus_tag="ACHK01_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8095160.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLESMEINGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMLALNVPVVPAIPQQ"
     gene            complement(64760..65935)
                     /gene="manA"
                     /locus_tag="ACHK01_000145"
     CDS             complement(64760..65935)
                     /gene="manA"
                     /locus_tag="ACHK01_000145"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO8095161.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66134..67780
                     /gene="fumB"
                     /locus_tag="ACHK01_000146"
     CDS             66134..67780
                     /gene="fumB"
                     /locus_tag="ACHK01_000146"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8095162.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67923..69326
                     /gene="fumC"
                     /locus_tag="ACHK01_000147"
     CDS             67923..69326
                     /gene="fumC"
                     /locus_tag="ACHK01_000147"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO8095163.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69323..70252)
                     /gene="tus"
                     /locus_tag="ACHK01_000148"
     CDS             complement(69323..70252)
                     /gene="tus"
                     /locus_tag="ACHK01_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO8095164.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70328..71633)
                     /gene="rstB"
                     /locus_tag="ACHK01_000149"
                     /pseudo
     CDS             complement(70328..71633)
                     /gene="rstB"
                     /locus_tag="ACHK01_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
     gene            complement(71637..72356)
                     /gene="rstA"
                     /locus_tag="ACHK01_000150"
     CDS             complement(71637..72356)
                     /gene="rstA"
                     /locus_tag="ACHK01_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO8095165.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72485..72820
                     /gene="ydgC"
                     /locus_tag="ACHK01_000151"
     CDS             72485..72820
                     /gene="ydgC"
                     /locus_tag="ACHK01_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO8095166.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72817..73539)
                     /gene="folM"
                     /locus_tag="ACHK01_000152"
     CDS             complement(72817..73539)
                     /gene="folM"
                     /locus_tag="ACHK01_000152"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO8095167.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73576..74958)
                     /gene="ydgI"
                     /locus_tag="ACHK01_000153"
     CDS             complement(73576..74958)
                     /gene="ydgI"
                     /locus_tag="ACHK01_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8095168.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75144..76088)
                     /gene="ydgH"
                     /locus_tag="ACHK01_000154"
     CDS             complement(75144..76088)
                     /gene="ydgH"
                     /locus_tag="ACHK01_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO8095169.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76612..78144
                     /gene="pntA"
                     /locus_tag="ACHK01_000155"
     CDS             76612..78144
                     /gene="pntA"
                     /locus_tag="ACHK01_000155"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO8095170.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKTAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78155..79543
                     /gene="pntB"
                     /locus_tag="ACHK01_000156"
     CDS             78155..79543
                     /gene="pntB"
                     /locus_tag="ACHK01_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO8095171.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79568..80473)
                     /gene="tqsA"
                     /locus_tag="ACHK01_000157"
                     /pseudo
     CDS             complement(79568..80473)
                     /gene="tqsA"
                     /locus_tag="ACHK01_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(80480..80662)
                     /locus_tag="ACHK01_000158"
     CDS             complement(80480..80662)
                     /locus_tag="ACHK01_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095172.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            80650..81879
                     /locus_tag="ACHK01_000159"
     CDS             80650..81879
                     /locus_tag="ACHK01_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8095173.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            82245..82433
                     /locus_tag="ACHK01_000160"
     CDS             82245..82433
                     /locus_tag="ACHK01_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8095174.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            82491..82991
                     /locus_tag="ACHK01_000161"
                     /pseudo
     CDS             82491..82991
                     /locus_tag="ACHK01_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(82934..83113)
                     /locus_tag="ACHK01_000162"
     CDS             complement(82934..83113)
                     /locus_tag="ACHK01_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8095175.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKEPE"
     gene            83313..83513
                     /locus_tag="ACHK01_000163"
     CDS             83313..83513
                     /locus_tag="ACHK01_000163"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO8095176.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVGL"
     gene            83510..83740
                     /locus_tag="ACHK01_000164"
     CDS             83510..83740
                     /locus_tag="ACHK01_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095177.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            83730..83954
                     /locus_tag="ACHK01_000165"
     CDS             83730..83954
                     /locus_tag="ACHK01_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095178.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLAFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            83947..84312
                     /locus_tag="ACHK01_000166"
     CDS             83947..84312
                     /locus_tag="ACHK01_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095179.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
     gene            84305..84526
                     /locus_tag="ACHK01_000167"
     CDS             84305..84526
                     /locus_tag="ACHK01_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095180.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISKLIRASFFLIGTKEYK
                     STGIDVLNIAADYADFVTEVILRKTTDGD"
     gene            84528..84761
                     /locus_tag="ACHK01_000168"
     CDS             84528..84761
                     /locus_tag="ACHK01_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095181.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWHETLGDNECDKQKSR
                     IVTALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            84767..85066
                     /locus_tag="ACHK01_000169"
     CDS             84767..85066
                     /locus_tag="ACHK01_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8095182.1"
                     /translation="MKLKYPGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIRKMLNVFRGKK"
     gene            85063..>86702
                     /locus_tag="ACHK01_000170"
     CDS             85063..>86702
                     /locus_tag="ACHK01_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO8095183.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLARERQNLSEGLFVELPVKQKAQLSQMADSERAQLLSDRF
                     DGVCVHPESEIVHVWRGGLWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLTTNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDKDLPEKVAAE
                     IPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVADF"
BASE COUNT        21984 a        21240 c        22601 g        20877 t
ORIGIN      
        1 aattttaatt gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct
       61 aaccataaag gattagttta tcggcgttaa atcttttctc atacactatc gggttaggga
      121 aaatatctgg tgttggggac agagtcagca gcgtattagt tgaatgctta atatcaggct
      181 gctgaattgt cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg
      241 tccactatta gatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct
      301 tgctggtcta acaattgccc ctgcgttact gtcacttttg ggttgcaaac aggaagatat
      361 tgatagcggc acagtagggt tgataaatac acccaaaggg gtgctggtga cccagcgagc
      421 gcgatgcacg ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt
      481 agggacattc ttctcccgta tcaaaatcca ccgcaattat ttctttggcg acaacggggt
      541 tggctctggc ggcggcctgt atggcgatct caactatacc gcggacacct gccgtcaatg
      601 caaagagccg caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga
      661 aggctgtatt accgtcgatc ataaacgctg tattggctgt agcgcctgta ctacggcatg
      721 cccgtggatg atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg
      781 cggtgaatgc gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat
      841 tactgtgtga atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta
      901 agagtcaatc tcacgacagg aaatattacc ctcgaagatt ccagtaagtt taaaagtttt
      961 gtcggtggca tgggcttcgg ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa
     1021 cctttcgatg aagcgaataa attagtcttt gctaccggcc cattaactgg atctggtgcc
     1081 ccctgtagtt ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtt
     1141 gtcgatgccc atatgggtgg cttttttgca gcgcaaatga aattcgctgg atacgacgtc
     1201 attattatcg aagggaaggc gaaatcaccg gtatggctga agattaaaga tgacaaagtt
     1261 agcctggaaa aagccgattt cttatgggga aaagggacgc gcgcaacgac ggaagaaatt
     1321 tgtcgattga ccagtccgga aacctgtgtg gcggctattg gtcaggctgg ggaaaacctt
     1381 gttcctctct ctggcatgtt gaatagccgt aaccacagcg gcggtgcggg aactggcgca
     1441 attatgggtt cgaaaaacct gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt
     1501 gccgatcgtc aggagatgaa gcgtctcaac gattacatga tgactgaact tattggtgcg
     1561 aataacaacc atgtcgtgcc aagtacgccg caatcgtggg cagagtattc agatcccaag
     1621 tcacgttgga cagcacgtaa agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa
     1681 acgggggaaa ttccgccagg caatcagaac acggtcggct ttcgtaccta taaatccgtt
     1741 tttgacttag gtccggcggc agagaaatac acagtaaaaa tgagtggctg ccactcttgc
     1801 ccgatccgtt gtatgaccca aatgaatatt cctcgggtga aagagtttgg cgtgcccagc
     1861 acaggtggta acacttgtgt agccaacttt gtccatacca ccatcttccc gaacgggccg
     1921 aaagattttg aagataaaga cgatggtcgt gtgattggta acctggttgg tctgaatttg
     1981 ttcgatgact acggcctatg gtgtaactac gggcagctgc atcgcgactt tacttattgt
     2041 tacagcaaag gggtgttcaa gcgtgttctg ccagctgaag agtatgcgga aattcactgg
     2101 gatcaactgg aagcgggtga cgttaatttc attaaagatt tttactacct tctggcgcat
     2161 cgtgtgggtg agctgagtca cctggctgat ggttcatatg ccatcgcaga acgctggaat
     2221 ttgggtgaag agtactgggg ctacgcaaaa aataaactct ggtcgccgtt tggctatccg
     2281 gttcaccatg ccaatgaagc gtcagcgcag gtcggttcca ttgttaactg tatgttcaac
     2341 cgtgactgca tgacgcatac ccatatcaat tttattggtt cgggtttgcc attgaaactg
     2401 cagcgtgaag tggcgaaaga gctttttggt tctgaagatg cctacgatga aaccaaaaac
     2461 tacacgccaa tcaatgacgc aaaaattaaa tatgcaaagt ggtcgctgtt gcgggtctgt
     2521 ttgcataacg ccgtcaccct gtgcaactgg gtctggccaa tgaccgtttc gccgctgaaa
     2581 agccgtaatt accggggcga tctggcgctt gaagccaaat tcttcaaagc gatcaccggc
     2641 gaagagatga ctcaggaaaa attagattta gctgcagagc gtatctttac gttgcatcgt
     2701 gcctacacgg taaaactgat gcaaaccaaa gatatgcgta atgaacacga tcttatctgt
     2761 tcctgggtat tcgacaagga tccgcagatc ccggtcttta ctgaaggtac tgacaaaatg
     2821 gatcgtgacg atatgcatgc ttcgctcacg atgttctaca aagaaatggg ctgggaccca
     2881 cagcttggtt gtccgaccca cgaaacattg cagcgtctgg ggctggaaga tattgccgcc
     2941 gatctggcag cacacaatct actgcctgcg taaggaatgg gtgagatgaa ccatcgggat
     3001 gaattgccgt tagcgaaagt cagcgaagtt gacgaggcga agcggcaatg gttacagggg
     3061 atgcgtcacc ccgttgatac ggtaaccgaa ccagaaccgg ctgaaatcct ggcagagttc
     3121 attcgccagc attctgtggc tgggcagctg gtggcgcgcg ccgtgtttct ctcgccaccc
     3181 tattcggttg cagaagagga actgtcagtt ttgctggaaa gcattaagca gaacggagac
     3241 tacgcggata tcgcttgtat gacgggaagt caggatgatt actattactc aacgcaggcc
     3301 atgagtgaaa actacgcggc gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc
     3361 attgcacacg ccgttcgttt tgagtgccag acgtatcctc gtccttacaa agtcgccatg
     3421 ctgatgcaag cgccatatta cttccaggaa gcgcagattg aagccgcgat cgccgcgatg
     3481 gatgttgcac ctgaatatgc cgatatacgg caggtggagt catcgacggc ggtgttgtac
     3541 ttgtttagcg agcgatttat gacctatggc aaggcttatg gcttatgcga atggttcgag
     3601 gtagagcagt ttcagaaccc ctgaaatttc agggagatgt catggatagg gtggacagtt
     3661 gcagccactg cattagggga taatcagatg tccttcactc gacgcaaatt tgttctgggg
     3721 atgggaacag taatcttttt tactggttct gcgtcttctc tgttagcgaa cacgaggcaa
     3781 gaaaaggaag ttcggtatgc catgattcat gacgagtcac ggtgcaatgg ctgtaatatt
     3841 tgcgcccgcg catgccgtaa aactaaccac gtccccgctc agggaagccg tttatcgata
     3901 gcacacattc cagtcaccga taacgataac gaaacgcagt atcacttttt tcgccagtca
     3961 tgtcagcact gcgaagatgc tccgtgcatc gacgtatgcc cgacaggggc gtcgtggcgt
     4021 gatgagcagg ggatcgtgcg ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt
     4081 ggcgcttgtc cgtaccaggt tcgctacctt aaccctgtca ctaaagtggc agataaatgt
     4141 gatttctgcg ctgagtcccg actggcaaaa ggctttccgc ccatttgcgt cagcgcctgc
     4201 ccggaacacg cattgatctt tggtcgcgaa gatagcccag aaattcaggc gtggttgcag
     4261 caaaataaat actatcaata tcagctacct ggtgccggga aaccgcatct gtatcgccgg
     4321 ttcggtcaac atttgattaa aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt
     4381 ttcagagcca gttagcgaac tatgtgccac agttcactcc cgaattttgg ccggtgtggt
     4441 tgatcattgc cggagtattg ctggttggga tgtggttggt gctggggctg catgccttgc
     4501 ttcgtgctcg tggcgtgaag aaatcagtca ccgattatgg tgagaagatt tatctttact
     4561 gcaaagcggt cagattatgg cactggtcga atgcgttact ctttgtattg ttgctggcaa
     4621 gtgggctgat aaatcacttt gcgctggtgg gcgcaactgc ggttaaaagt ctggttgcgg
     4681 tgcatgaagt ttgcggattt ttgttactcg catgctggct cggctttgtg ctgatcaatg
     4741 ccgttggggg taatggtcac cactatcgca ttcgtcgtca ggggtggctg gagcgagcgg
     4801 caaaacaaac gcgattttat ttgtttggca ttatgcaggg ggaagaacat cctttcccgg
     4861 caacaaccca gtctaaattt aatcccttac agcaggtcgc ctatgttggt gttatgtatg
     4921 gattgctgcc gttgttacta ttgacggggc tgctgtgtct ctatccgcaa gccgtgggag
     4981 atgtgtttcc tggcgtaaga tactggttat tgcaggcgca ttttgctctg gcatttataa
     5041 gcctcttttt tatcttcggt catctttatc tttgcaccac ggggcgtacg ccacacgaaa
     5101 cctttaaaag catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag
     5161 acctgcgaga atggcgtatc ggagcggtga tgtatcgctg gttcctgcgc catttcccac
     5221 gcggcggtag ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg
     5281 cggagagtct ggttgagtat gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc
     5341 aggaagtttc ctctatgcaa aaactggcca cagaccccgg agagatacca ttttgcagtc
     5401 agtttgcccg cagcgacgat catgctcgca tcggctgttg tgaggataac gcccgaatcg
     5461 caaccgctgg gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg
     5521 tcggctttaa cagtcacatt ggcagctccg gtgaacgcgc acgggttgct gtaaccggta
     5581 attcttcgcg gataagcagc gctggagata gtagtcggat tgcgaatacc ggaatgcgag
     5641 tacgcgtttg tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa
     5701 tcgccagctt tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg
     5761 ccagcggtga aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc
     5821 cagggggaag cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca
     5881 ttgaaggtga aaacaatatt cgaaccggag tccgctatcg tcttaatgaa cagcatcagt
     5941 ttgttgagtg ctgatagctt tacctttaat caaacgaaag acgccgacga gcacgggatg
     6001 caggctttac gaccgcccga gccatcgcct caccaatttc tgcacatgcc gtaagtccct
     6061 gaacgaaagg ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa
     6121 tatcttcggg ttgctgtaac gtataatctt cgccaacatc agggatttca tcccctgttt
     6181 tctgtaattg ctcgcgtagc ccagggaaag gaatatcttt cactttaagc ggacgctgtc
     6241 cgcgggcatc aataaaaacg tcaaacgagt agctgttgtc ttccgttttc aggacggtgc
     6301 gcgactcatt aatttccatt ttgtagtctt caccgagggc gagaatatga atgattcccg
     6361 cttcacgtaa ggcaagtagg cgacgaatag actctgaagg gattgccgca taattatcga
     6421 tgaaaaccct ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgaggaa
     6481 caatttcctg tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt
     6541 tttctcgttt attgcgttcg acttcctgga gatttttttc tgcccagtca aatgggtcgc
     6601 gttgtttgcg ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg
     6661 ctatgcgttg gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata
     6721 cccgatccaa aaggccctct tcaccttttt gaatctcagc atttaatgcc tgatcggtga
     6781 cgatgtgtaa gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacccg
     6841 tgcgcgacat caacgtaata tttagctttt cacttgcgtt atcgcggtta aaaacgacgt
     6901 gttgtttatc atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca
     6961 gtccgctcaa ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca
     7021 ggcctgacca cggactggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt
     7081 gacccgtggc aatcaccgct aaatcaaacg tctcgctggg taaatcctga tttgtagcga
     7141 gcatgacgcc agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca
     7201 ctgcaaattt ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt
     7261 cgcccagcag aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat
     7321 aacgctggag atggctggct tcttgctttt gtagccattc gagatacgta caattaatcg
     7381 gcggtatttc aatactggca atatttgcca gcatcatttt tgagttttcc tcatcactgt
     7441 atggcatccc gacaccggcc tcgtcagcct gctcgaagat agaaatagaa agtggagttt
     7501 gttgctgtag aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa
     7561 tttttttcat tgttacttac ctgtacgcat tctttcgaat ttagtgttat cagataagtt
     7621 tggttgagat ttttagaatt tgagaaattt cagacaggga tgaaaagggc gctaaccgcc
     7681 cttgattaaa ttctgtcagg cgagttttgc ctgattgatt tgactcagtg gctcattgac
     7741 atcgaaaact ttggcgacaa cttcctcaac gccgagattt ttaagtcggg cagtatgttt
     7801 ttccagatag gcaagggcgc ttttttcatc agtaaaaaga tagatcccac cggcttcgtg
     7861 gttcttttca ctttctgtcc ataccttcca cagaaaacca ggttcctgat taatcgactc
     7921 agcaagcggt ttaagctgct cagccattgc gtcgccaaat gggccattaa aagcaaaatg
     7981 aagttgtaac aaagttgcca tagcgattct ccttctgata acgcatacga agacagtgta
     8041 aatcttttaa cgtattggat acagagaaga aacagaggga aaaaggagaa gaccagatta
     8101 agaaaatctg gtgcgtccga gtggactcga accaccgacc cccaccatgt caaggtggtg
     8161 ctctaaccaa ctgagctacg gacgcaggat ggtgcgttca attggactcg aaccaacgac
     8221 ccccaccatg tcaaggtggt gctctaacca actgagctat gaacgcaacg ttgtaggtga
     8281 caacggggac gaatattagc ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc
     8341 tgcgctattt cgaccgtttg cagagctttt aagcaaattg gctatatttt gttgatttgc
     8401 aagggcgatt tttattcagg atcgcattta catctgatac aacccaataa ttcgtgtatc
     8461 agagtttaaa cagagttgtt tgccaccatc tcaaatctgt cgtggataaa tcctccgtag
     8521 cctttacact taggtagcaa taaaggcaca cagatgcagc aaaggaataa atcatgggat
     8581 ctgatgcgaa aaacttgatg agcgacggga atgtgcaaat tgttaagacc ggcgaggtca
     8641 ttggcgcgac gcaacttact gaaggcgagt tgattgttga agctggcgga agagccgaaa
     8701 ataccgtggt cacgggggct ggctggttga aagtggcaac cggtgggatc gccaaatgca
     8761 cacagtacgg taacaatggc acgctatcgg tcagcgacgg tgccattgcc acagatattg
     8821 ttcagtccga gggaggcgca attagtctct ctacgctcgc tacggttaat ggccgccatc
     8881 ccgaaggtga attcagcgtt gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg
     8941 gtaacctgcg tgtactggaa ggacatcgcg cggaaaaaat cattctcgat caagagggcg
     9001 gcctgttggt taatgggaca acctcagcgg tcgtggtaga tgaaggtggt gaattgttgg
     9061 tgtatccagg tggggaagcc agcaattgtg agattaatca gggcggcgtt tttatgctgg
     9121 ccgggaaagc cagtgatacg ttgcttgctg gtggcaccat gaataatctc ggtggtgaag
     9181 actctgacac tattgttgag aatggatcca tttatcgtct ggggacggat ggccttcagc
     9241 tctacagttc cggtaagacg caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc
     9301 atgccggtac gctggaaaat gcggtaatac aaggtggaac agtgatcctg ttgtcaccca
     9361 ccagcgcgga cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa ctgaccggga
     9421 gtgttgcatt actggacggc gcttcaatga ttattggcta tggcgcagat ctgcaacaat
     9481 caacgattac tgtacagcag ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg
     9541 acggtgtcac ttttattgtt ggtaacatca atctgaatgg cggaaaactg tggctgatca
     9601 ctggtgcggc aacgcatgtg caactgaaag tgaaacgcct gcgcggagag ggagcgattt
     9661 gcctgcaaac cagtgcgaaa gaaatctcac ctgacttcat caatgtgaaa ggggaagtta
     9721 ccggggatat acacgttgag ataacagatg ccagtcggca aactctgtgc aacgctctga
     9781 aattacagcc agacgaagac gggattggcg caacgctcca gcctgcgtaa aaacacaggc
     9841 gcaacccgtg aaggttgcgc cttgtcttta ttagcgggat gctcgttgca gaatgataac
     9901 tgacggcaga cgttgcagga accgcatacg cagcatcatc ataatggctg ccgacgtcag
     9961 gccaataata aagcctatcc agaagcctgc tggccccata ggttcaacga ccagatcggt
    10021 cagtgccaga atatagccgc ttggcaagcc cagcacccag taagccgtaa aggtgatata
    10081 gaaaatggaa cgcgtatctt tataaccacg caaaatccca ctgccaatca cctggattga
    10141 gtcagaaatc tgatataccg ccgccagcaa catcaaatgc gcagccagcg ttacaacctc
    10201 gggattgtcg ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga atatggccgt
    10261 cagggttgcc atacagacac ccaccataag cccggtccgc gcagcggttt gcgcatccag
    10321 cgttgagccc tgacccagac gataacctac gcggatagtt accgctgccg ccagcgacat
    10381 tggaagcaca aacatcagtg aactaaagtt cagggcaatc tggtgtcctg cgacatcaac
    10441 aataccgagc ggagacacta acagagccac gacggcaaac agtgtcactt caaagaatag
    10501 tgccagcgca atcggcaaac cgagttgaat cagtcgtttc ataaccgcag gatcgggttt
    10561 cgcggtgcct ttttcgttac gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac
    10621 catggcaagg aacatgaccc aatacaccgc cgcagtagcc acgccacaac caacgccacc
    10681 gagctcaggc ataccgaaat gaccataaat aaagatatag ttcaccggaa tgttcaccag
    10741 caggccgata aagcccatta ccatacccgg cttggttttt gccagacctt cacattggtt
    10801 acgggcaacc tggaagaaca gatagcccgg cgcgccccac aacaacgcac gcagataacc
    10861 cacggctttg tccgccagag ccggatcgat gttttccatg gagcggataa tgtaacctgc
    10921 attccacagc acaagcataa tgagaacgga aacaaaacct gccagccaga aaccttgtcg
    10981 cacctgatgc gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga taaccggcgt
    11041 taatgccagc agcagtccgt gaccaaagag gatcgccgga agccagatag aagtaccgat
    11101 agcgaccgcc gccatgtcgg tggcactata gccgcccgcc atcacggtat cgacaaaacc
    11161 catcgcagtt tgggcgattt gcgcgagaat caccgggatt gctaatgcta ataacagacg
    11221 cgcttcactg atatacttct gcacgtgaac accttttatt tgtagttata tgagagacta
    11281 aaaagccgcc tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg
    11341 aggatttatt aattctccag tgaaaaaata gtcattctta ccccttcact ggtaagggcg
    11401 tttgccacct gctattgtgc tgaacagaat gtcttaactg atttcaggag ttgtaagtat
    11461 gtttacgggg attgtacagg gcaccgcaaa actggtgtcg attgacgaga aaccaaattt
    11521 tcgtacgcat gtggtggagt tacccgacca catgctggac ggcctggaaa caggtgcttc
    11581 cgtggcgcat aacggttgct gcctgaccgt gacggaaatt aacggcaacc atgtcagttt
    11641 tgacctgatg aaagaaacgt tacgcattac caatcttggc gatttaaaag tgggggattg
    11701 ggtaaacgtt gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact taatgtcagg
    11761 tcatattatg accaccgctg aagtggcgaa aatattaacc tcggaaaata atcgccagat
    11821 ctggtttaaa gtccaggata gtcagttgat gaaatatatc ctgtacaaag gatttattgg
    11881 catcgacggt attagcctga ccgtcggcga agtcacgcca acgcgttttt gcgtccattt
    11941 aattccggaa actctggaac gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa
    12001 cattgaaatt gatccacaaa ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc
    12061 acgagaaaat gccatgaatc aaccaggtac agaagcctga tctgaaatcg cccccggaga
    12121 gggggcgata gaatagcctt tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga
    12181 acacgacctg ccagagttga atatcacggg cgcggaaagc acctgcacag gcattcagat
    12241 aataggtaaa cattcgttta aagcgttcac tgtagttatc cgcaatttct ggccatgcgg
    12301 cgaggaatcg ttcataccac gccatcaacg tagtatcgta atcagcaccg aagttatgcc
    12361 agtcttccat cacgaagtgg ggttcgctgg attgagcaat ctggcgtaca gagggcaggc
    12421 aaccgttcgg aaaaatatat ttattaatcc agggatcaac attcagatcg gtttttttcg
    12481 aaccgatagt atggagcagg aatatacctt ccggtttcaa attacgatcc accaccgcaa
    12541 aataggtatc gtaatttttc ggtccgacgt gctcgaacat ccccacagaa acaatacgat
    12601 caaactggtc gttcaggtca cgataatctt gcagcaaaat ggtgacatcc aggccttcac
    12661 agcgttcctg agccattttt tgctgttcgg cagaaatggt gacgccgacc acgcttacgt
    12721 catagttaga tgccatgtag tgcgccagtc cgccccagcc gcagccaata tccagtacgc
    12781 gcatccctgg ttttaactgc aatttttcac aaatcatttt gagcttcgcc tgctgggcag
    12841 attccagatt atcggcatct ttccagtaag cgcaggaata ttgcatgaag ggatcaagca
    12901 tgcggctgaa caagtcatta cccaaatcgt aatgctcctt gccgactatc caggcacgtt
    12961 ttttactctg cagattgaag agacgagcgc tggcaatacg cagcgtgtct ttgaaatgat
    13021 gggggagttg gttctcgaga cctgcgcgta agactttgct aaaaaacata tccagtcggt
    13081 cacattccca ccagccatcc atataacttt cgcctaaccc caaagagcct tcttgcagaa
    13141 cgcgtttaaa aaaatcgggg tttttcacac gaatatccgc cggggcagaa ccgttaatgg
    13201 ctataccggc acggctaagt aattcgttgg cgatacggta ccagttgtca tccggtacac
    13261 tgacttcttc tatacacgat gaactcatag tttctccatc actggtcggt gatcagaacc
    13321 ttaaaacagc gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt
    13381 ctataatccg acgtagaaca gagggaaagg aggaaccttt gccgaaatga ccatccatag
    13441 tgagtcggga acgatcctgt ccccgtaaaa acataatatt tatcgtagct ttaacaactc
    13501 gaaattagta taggccgcta atttcaatca tacaacaaaa ttttgttagc agcctaagta
    13561 taagtatatc gatatagatc agtgtgattc gctatgagcg acttcggcat tgccatgatt
    13621 ctggcagcca acttcttcac aacgttgcat catgtaaccc agagcgacca gcactactgt
    13681 tgataacatc acgctggtgg tggtgagcaa tggcgtgctg atactgatca gccaggaaac
    13741 taccagactt gcgaggaagc acagacccag ttgtagagtg ttctgcaacg ctgcggcgcg
    13801 accagttgcg tgtgggaagg gacgcagcgc ctgggcgaca acaatcgggt agatcgcgcc
    13861 attggcaatc gccatcacac agaatgggat caggatttcg accagcgaca catggctaat
    13921 gaagcctgca gcccaggtcg caatgacgct gactgcaaac agcaccagca accacggtaa
    13981 taactgcttg ccttgccatt tctgcagcgc ggcgcgacag ccataaccac caatcagaaa
    14041 cgcgatagtt tgcgggacat aacttaaacc aataactgcc gggctgtagc ccatttcact
    14101 aaggatgaac ggtgaaccgg tcagccatgc aaaaaaactg gctgaacagg ccgcgtatat
    14161 cagcacgttg ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg
    14221 actattgtta cgggccttcg tcgtgggttt gagccagaaa ataggcagaa tcagcaccac
    14281 ggtaatggca aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca gcagccagct
    14341 tcctaacaga ggagccaggg ccggagatag acccaccagc ggcatgatgg tcgcaaaaat
    14401 tcggttaact ttctgtgaag gataataatc cgtcactaac gcttgccaga taaccgccgc
    14461 ggcgcagaca cccacagcct gtacaaaacg caataccagc agcgtagcgg cgttttctac
    14521 ccacagcatc cccagactac ctaacgcaaa aattgtcagg ccgattaata acaccggttt
    14581 acgaccataa cggtcggaga gtggccccca cagaagctgg gctgcggcaa aaccggcaag
    14641 gaacagacta aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc
    14701 ggcgaaagca ggcagataca tatcggttgc cagaaaaccg agtacgctca aacccgccag
    14761 ccagactaaa aatcttttcc caggttgcat gtatattctc taaatctctc aattaaacac
    14821 cgccgcaaag tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg atgactggtt
    14881 tcaaaaattt tgcaggcaga ctatgtggtc agaatattca ctcgaagttg ttgatgcggt
    14941 agcgcgtaat ggtagtttta gcgctgcggc acaggagctg catcgcgttc cttctgcggt
    15001 cagctatacc gtgcgtcagc tggaagagtg gctggcggtg ccgctctttg aacgacggca
    15061 ccgtgatgtg gagctgaccg ctgctggagc gtggtttctc aaagaagggc gctctgttgt
    15121 caaaaaaatg cagatcaccc gccagcaatg tcagcagata gcgaacggct ggcgcggtca
    15181 gttagctatc gcagtggata atattgtcag gccagaacgt acacggcaga tgatcgttga
    15241 tttttatcgc cattttgatg atgtcgaact tcttgtcttt caggaagtgt tcaacggtgt
    15301 ctgggatgcg ctttccgacg ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc
    15361 ggtaggcggt cgttatgcct tccgggatat ggggatgcta agctggagtt gcgttgttgc
    15421 cagccaccac ccgctggcgt tgatgaatgg cccattcagc gatgatacgt tgcgcaactg
    15481 gccgtcgttg gtgcgcgaag acacctcgcg aacgctacct aaacgtatta cctggttgct
    15541 ggataaccaa aaaagagttg tcgtgcctga ctgggaatca tcggcaacct gtatctcggc
    15601 aggattatgc atagggatgg tgccaacaca tttcgccaaa ccgtggctta atgaggggaa
    15661 gtgggtagca cttgaattgg aaaacccctt cccggattcg gcatgttgtc tgacatggca
    15721 gcaaaatgat atgtcgcctg cgttaacctg gctgcttgag tatctcggcg atagtgagac
    15781 gctgaataag gagtggctgc gggagccgga agagactccc gcaacgggtg attaacgacg
    15841 atagtcgcgg aacgggccgt cagccacgga gcggcgttca atcaagcgcg gatgcacttc
    15901 aatggactgc ggttcttcac gtttgttgac gatacgatcc aacagcatgt tgaacgctgt
    15961 ttcacccagc gaatcttttg gctggtggat cgtggtcagc gccggcgtaa aatagcgggc
    16021 gttgcgcacg ttatcataac cgatcagcga aacatcctgc gggacgcgca gacccatttc
    16081 atcagcagca caaagtgcgc ccattgccat gatatcgcca ccacagaaga cggcagtagg
    16141 gcgatgcggc tgcgacagta tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc
    16201 accctgaaca atccagcttt ccggcacctt gatcatcgct tcttccatcg ccttcataaa
    16261 accggcaagg cggcctgcgc cggtgttacg ttccagcggg ccggggatga cgccgatttc
    16321 gcggtgaccg cgttcaatca gataacgccc ggccatgtag ccgccttcga acgcgttatc
    16381 aatgaccgca tcggtgaagt cagcttttgc ttcaccccag tccatcacca ccattgggat
    16441 atggcgatac tcttccagca tcgccagcaa cggctctggg tactcagaac acatcaccag
    16501 cagaccatcg acgcgttttt gcgccatcat cgacagataa gcccgctgtt tctcaagatt
    16561 gttccacgca ttacccagaa tcagggtgta acctttctgg aagcaatttt tttcaaccgc
    16621 ttcaatgatc tcggcaaaat aggccgcttc gctgctggtc gccagcaaac cgatagactt
    16681 ggtgtggtta accttcaggc tacgcgccac cgcgctaggg gagtagtgta attctttaat
    16741 cgctgcccac acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg
    16801 tgacacagtt gtagtggaaa cgtttgctcg tttcgctaca tcttttattg ttgccattcc
    16861 atttcactcc agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta
    16921 cacaccttac gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg
    16981 aaggggtcaa tggccagtac gcaaatagtg taagcgtgga attttggccg atcttgacga
    17041 aaaggggaag tggtaaaaac acttatcatt ataaatgacg ggagattttt tcatcacagt
    17101 gtgtaaaaat gcgtaatatc actatttgtg tggtaataaa aaaggagaaa acttgatgag
    17161 caccgacctt aaattttcac tggtaacaac gattatcgtc ctcggtttga tcgtggccgt
    17221 gggtttgact gccgcgctgc actgatttcc tctcgaggga gcgcgttctc cctcgcatct
    17281 ccttcctgtt tgttagcaaa atggcaaaag tgttttctct ttttgttatt ttcatttttt
    17341 tgtgattgct gtcatgcttt cgccacttta atgaatatcg cgtaatgacg cgaagaggtg
    17401 gagataaggc atgaaaatta actatccgtt gctggcgctg gcgattggcg cgtttggtat
    17461 cgggacaacg gagttctcgc caatgggctt gttgcccgtc attgcgcgcg gtgtggatgt
    17521 ctcgattccc gctgccggaa tgttaatcag tgcctatgca gttggcgtaa tggttggcgc
    17581 gccgctgatg acgcttctac tttctcatcg tgcccgccgc agtgcgttga ttttcctgat
    17641 ggcaattttc acgctcggca acgtactttc cgccatcgcg ccggattata tgaccctgat
    17701 gctttcacgc attttgacca gcctgaatca cggagcattt tttggtttgg gttcagtcgt
    17761 ggccgcaagc gtggtgccaa aacataaaca ggccagcgca gttgccacta tgtttatggg
    17821 gttaaccctg gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg aaaccatcgg
    17881 ctggcggatg tcatttctgg caacggcggg gctgggagtg atttcaatgg taagtctgtt
    17941 cttctcatta cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt
    18001 attaatgcgt ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag ctggtgcaat
    18061 gtttactctc tacacctata tctctccggt actacaaagt attacccacg caacaccagt
    18121 gttcgtcacg gcaatgctgg tgctgattgg tgtcggattc tctatcggta actatctcgg
    18181 cggcaaactg gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt tgctgctgat
    18241 ggtgattatg ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag ctattagcat
    18301 ggtggtgtgg ggcgcagcaa cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg
    18361 tgtcgccagt gaagcgccgg gtctgtcttc atcagtcaat attggtgcct ttaatcttgg
    18421 aaatgcgctg ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg gatacagctt
    18481 tgtgccggtg atgggagcga ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc
    18541 cagaaaacaa cctgaaacag tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg
    18601 ataaccttct gcgttacagt tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta
    18661 acgcctcatc cgcgttggca tggtaagcga agcgcatcag gcaatgctgc atttgccatc
    18721 agttattatg cagcgagatt tttcgctacg aactcccagt tcaccagcgc ccagaagtgc
    18781 tccagatagc caggacgtgc attgcgatag tcgatgtaat aagcgtgttc ccagacatca
    18841 acggtcagca gcggagtcgc atcggtggtc agcggagtac ccgcgttaga ggttgaaacg
    18901 atagccagtt tgccatcgct gtttttcacc agccaggtcc agccagaacc aaagtttttg
    18961 atcgctgcat cagtaaactg cgctttgaaa tcggcaaagc tgccaaaaga tgcggcgata
    19021 gcttcagcga cttttccagt cggttcgcca ccggcgttcg gtgccaggca gttccagtag
    19081 aaagtatggt tccagacctg agctgcgttg ttgaatacgc caccttcaga gctgcgaata
    19141 atctcttcca gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg
    19201 acataagtct gatgatgctt gccgtagtga tactcgatag tttccgcaga aatgtgcggt
    19261 gccagagcat ctttagcata tggtagtgca ggtaattcga atgacattgc tactctcctt
    19321 tattattaat ttgcatacgt acaatagcct tattgtgcgt atggataggg tagcaaatta
    19381 aaaggtgaga caaaagatga acttaccctg ttgccgtaac aacagggtaa agttttagcg
    19441 aagtgttttt ggggtcatta cccgacgagc gccaacatag tggcgctgcc agtagtcttc
    19501 actgagagaa gtgatttgaa tttcctgacc tgtgcgcggt gactgaataa atttgccgtt
    19561 gccgacatac acgccgacat gatcggctgt gccgcgtccc tgagtacgga aaaagaccag
    19621 gtcgccgttt ttcagttcac tacgttcgat tggcgctgca tcacgcaggt gatacatttc
    19681 attcgccgta cgcggaatac gaattttcac caaatcttta taagcgtaat aaaccaggcc
    19741 gctgcaatca aaaccggtac gcggtgagct gccaccccaa cgatatggct taccaatttg
    19801 ctgcatcagt ttattcattg ccactttcgt agctttttgc accttcgctt tgtgcgcgtc
    19861 ggcaagagtt cctgacgcgg catttttgac tttcacacaa tgcgatttat agcccttacg
    19921 tttggtacac ttttcggtaa agctggctga ggccgtttta ttgacggcgt gtttgctgcg
    19981 agttttggcg tttttagaag cggtaatgga agatttttta acggaagaag cggttttgct
    20041 tgtggtcgta cttttactgg cggctttttt cgctgttttc tgggtctttt tggtggttgc
    20101 cgtgcttttc tttttatctg cttttttggt gatatgagtg gtagcagaac tctccctggc
    20161 ttgctttgaa gcatgggcca taggcgttaa aggcagggtg gtaaaaagta aagcacagag
    20221 cgtgatcgaa atacggttta tccgcgccac tgagcgtccc tctgttaagg caggtctatc
    20281 atgcctgcgt ctgatttaca aaacttgatg attctaatgc atagaagaag agaaagttaa
    20341 tagcttttat tgttaaaaaa tacgtttcgt tggcgagcgc aaaaaaatgc agcagtgaaa
    20401 gctaaaaatg gttataaata cgccagtttg ccgcgcatca gagaagaaac gaatatttga
    20461 aatgcaacaa atagagcaaa gggataaaat agcaaaagcg tgatgaaaat gttattttcc
    20521 gatgccgcac tgacccgcta caatgtcaga ttactgccgt aaagaagttt gaggaagcaa
    20581 gacaatgagc accactatcg aaaaaatcca acgccagatt gctgaaaacc cgatcctgct
    20641 gtacatgaaa ggttcaccga aactgccgag ctgcggtttc tctgcccagg cagtccaggc
    20701 gcttgccgca tgtggcgaac gttttgccta tgttgatatt ctgcagaatc cggacattcg
    20761 tgcggaactg ccgaaatatg ctaactggcc gaccttcccg caactgtggg ttgacggcga
    20821 gctggtcggc ggttgtgata tcgtgatcga aatgtatcag cgtggcgaac tgcagcagct
    20881 gatcaaagaa actgccgcta aatacaagtc tgaagagccg gacgcggaat aatccgtcct
    20941 gcaatactga cggatgcgaa cgggcatccg tcagtccttt ctctatcccc aatccagccc
    21001 ttgtggcgaa cttgaaaatc ccgcctcacg cagcagcgta aacatcggcg tttgccggac
    21061 cggtagatca ttcacttctg ttagcgtaaa gcgcagccgt ggttcgcgac gcagtgcggt
    21121 agtcagcgcg tggaaaacct ctggggcgag taattcctct ttttcctgcc agaccagcat
    21181 ttttttgcca ccttgcgcca gatagagtaa caatttgccg ccagaaacga ccaccagcgc
    21241 acccgcccga cgcgttggaa ccagcgttgc cggatgtgca ggccagggca gaagatttcc
    21301 ccacacatta gccggatcgt tggcagagag cgccactggt gtatagtggc gcgtttgcgt
    21361 ggcttgtgtc gccagatcgc gcaatcggtc aatagtcaga cgttcagcga attgcgcgcc
    21421 acccagacct tctacaaaac gacctcgcat aattcgcccg gagtcttcca tacttcgaca
    21481 aagcgtttgc atcgatggaa acccgccagg gatattttcg gctatcaccg cctgacgact
    21541 gatgatgccg tagcggtcga gcatattttc cgccagcgcc agcatccttt cggtatcgtt
    21601 tagtggctcc acctgcaata acgaccagcg tccagccaga tttggtgtgt tgtaagatac
    21661 cagcggcgag acgggttgcg catagacagg acgtccacga cgagcctggt gactgcggcg
    21721 agttgaggtg cgtgcgttgg aactgctgcg ggtgagggcg cgtaacggtg cccaaatgtc
    21781 gctggtgatg acgccttgcc agaccagcgc ccataacgcc tcttgcaggg cagagagatc
    21841 aaccgattcg ccgattttgt cgcgtatccg ttggctgatt tgttgtgcaa accaggctcc
    21901 tccgtcagcc agaacagcga ctatcgcttg ttgcagcgcc gaacgattcg cctgatccgc
    21961 ttcggcggga gtgaacgatt ctgcagcata ttcctgtaga tgcagtgcca ccaggccgtc
    22021 atcttcaccc agtttttttt gccccgacca gataaccgca ccggttgcca gtaattcatc
    22081 gagcatttct gacgaatagt cgcgtacgcg ggcaggcagg atctggcttt cccagagtga
    22141 ggcgggtaaa ccgactccgg caagctgttc gatcacccgc atcacgccat ctacgccctc
    22201 ataaacgcct ggcgatgttg aggcaaagag cgccgggcta ccatcggtgg cgggtaatac
    22261 gccctgacgt tccagcagca atcgcgcata ggtcgtggct gcaacgggac gcgtcgcttc
    22321 tctggcggct tgcagcgagc gcaaacgcag acgacgaaat acttcatcgc tcacccagat
    22381 gtcttgttgc agattcatca ccagaccctg ttcacgcagt tgctgaagct gctcttcgac
    22441 aatggcaata ccgagactaa actcatgagc cagttgttca gccgtgacca gagcatgagc
    22501 ccggagatag cgcagaaaga gatcgcgaag cgggtaactg actctatgta aataaatctc
    22561 tggcaatgac tctggtagtc gtacgccgag ggcatcacgc agcctggcgg catcatccat
    22621 acaggcaaga cgctcctgtc cgctaatcat cgctgggaag attcgtttta ctgcaagaag
    22681 attttccaga tacgacgcaa cctcttcact gctgcctgta tgccgttgcg ccaggtcttc
    22741 aacggtcatt ggccccagtt cgcgcagcag gtcgaacaat ccttcttcac ctttcgctct
    22801 tctgccagga gccagtcgtt gcaactcttc ttccacctgg cgaatgacct gcgggtcgag
    22861 taattccccc ggatcgacct gtccgagtag attgcgcagt aactcgctgt ccagcgacag
    22921 tacggatgcc cggcgctctg ccagcggggc gtcgctctgg tacataaatt ccgcgacata
    22981 gccgaacaat aaacttgtgg cgaaaggcga gggcgtagtg gtcgttacat cggatatttg
    23041 aatttcgcca ccgttcaggc gacgcatcaa acgttccagt gcgggaagat cataaacatc
    23101 ttgcagacat tcgcgtaggg tttcgagaat gaccggaaaa tccggatatc cctgagcgat
    23161 ttccagcaac tgactggcgc gcagccgttg ttgccaaagc ggggtgcgat ggcccggagt
    23221 gcgccccggc attaataatg cccgcgcggc gcattcgcga aaacgggcgg cgaaaagtgc
    23281 cgagctgcct accgcctcgc ggacaatttg cagcaacttt tctggttcaa acaaaaaaat
    23341 cgcggcgtcg ggcaatttac catcagtgtc aggaatgcgc gcaacaatgc cgtcatcact
    23401 ggcgaccaca gacgcgtcag cgccccatag ggcatgtatt cgcccggcaa tcgccagcgc
    23461 ccagggttca tgcacccgtc ttccataggg agagtgcaaa ataatacgcc agtcgccaat
    23521 ttcatcacgg caccgttcga ggaccagatg gcgactcccc ggaacgatgc ccgtcgcgtt
    23581 gcgttgctcg tcaatcaacc cctgaatatt ggcgatcgta ttttcttctg ccagccacgg
    23641 gggaatagtc ccggaaaaga acgcgccatc cgccagcaaa tgaagaaaat cgccgatcat
    23701 ctcgcctaat tcagccggac gtccgttacc ttcaccacgc cagaagggga gccgggcaga
    23761 acgacccgga gcaggagtca caatcacctg atcgcgggtg atttgctgga tccgccatga
    23821 ggtagcgccg agagtgataa tgtcgttcac ccgcgactca tataccatct cctcatccag
    23881 ttcacccacc cgccgcgaac cggcctgttc ttcaccttcg ggtaataaca cgctatacat
    23941 gccacgatcc ggaatggtgc cgccgctggt aaccgccaac aattgagcgc caggtcgggc
    24001 ggtcaatatc ccggtctccc tgttccagac cagtttgggg cgaaaagcag aaaaatcgcc
    24061 agagggatag cgcccggaaa gcatatccag cgtggcgtca aaaacacgtc ttggcagatc
    24121 tttccacggt gcggcacggc gtacgcggga gtaccattcg tctacctgta atgcctccat
    24181 cgccgcggcg gcaacggttt gctgcgcaag gacgtcgagt ggattatgcg gcggtgtcag
    24241 gttttccagc ctgcctgcga acatacactc tacaatgact gcggaatcga ctaagtcacg
    24301 ccgggtacga gggaaaaaca gccctttaga tacaccgcca acctgatgtc cggcgcgacc
    24361 aatgcgttgt aacccactgg caacagaaag cggcgttgcc acctgaatca ccagatccac
    24421 tgcgcccata tcaatcccca gttcgagact ggaggttgcg accacgcagc gtaactcacc
    24481 cgatttcagc gcctgctcgg tgattgctcg ttgttcttta gagacggagc cgtggtgtga
    24541 gcgggcaata aaaacgtcgc tactttgtac acggttagag gttgcgccgg aggtcgactc
    24601 gaaatgggcc gcatcaacgg cgatagacgg ggaacgctgt aatcgtgcgg cgtaaagctc
    24661 atttaatcgt gccgtcagtt tttccgccag cccacgtgaa ttagtaaaga caatggtcga
    24721 gcgatggcgc aacacttcat caaggatacc cgtttcaata tatggccaga tggagccttc
    24781 ccggccggca tggctgtctt cgccggtgct gctggcgacc gatgagacat catccatatt
    24841 ggcgaccggt acgacaattc gtatctgcgg atggcgcatt gcgggcgggt tgactaccgt
    24901 aaccgggcga tcgccaccaa gaaatgctgc cacatcgctg gctgagcgca cagtggcaga
    24961 aaggccaatt cgctgtgctg aggtgtggag cagcgcatcg agccgctcca gacttaacgc
    25021 cagatgcgca ccacgtttac tgcccgctac cgcgtggact tcatcaataa ttaccgtttc
    25081 gacgccgcgt agcgtttcgc gcgcgcggga ggtcagcatc agatagagtg attcgggtgt
    25141 ggtgatcaga atatccggcg gattacgggt gagtttgctg cgttcctgtg caggcgtatc
    25201 gccagtacgg atccctacgc gaagattgac ttccgtttcg ccgcgccgcc gccgttcatc
    25261 ggcaataccc ttcaacggga gctgcaagtt gcgctgaacg tcggtgccca gagcttttat
    25321 cggtgaaata tagaggatgc gtgaggtttt acgcttatgc gcctcgcggg tatcttcgcc
    25381 gccttcgcgg aagagccgat cgagggcgta gagaaatgct gccagcgttt tcccggagcc
    25441 ggtcggtgca atcaccagcg catgttcgct tcgcgccgcc acatgccagg tttgcgactg
    25501 gacagcggtc ggctgtttaa aggcgcgaag aaaccagtcg cgggtcgccg gtgaaaacac
    25561 gtccggcagg agcgatgaag agtctggatt atctgccatt aacgctattc cccgtgaata
    25621 tcatgcagga tgcgtggaat agcctcacta tagatgcctg aatcattgca tgtcatcagg
    25681 catcgactcg attacacctc ttcggcggca ggtagcggcc agcctcccag acgtttccag
    25741 cggttgacga tttcacaaaa cagcacagca gtgcgttcgg tgtcgtacag cgcggagtgc
    25801 gcctgggtgc tgtcgaagtc catgccagcg gtctggcaag cctttgacaa tacggtttgt
    25861 ccgagtgcca gcccggccag tgcagcagtg tcaaaagtgg cgaaagggtg gaacgggtta
    25921 cgtttcagtg aggcgcgttc tgcggcggcc atcataaagc tgtgatcaaa attggcattg
    25981 tgcgccacca taatggcgcg gttacagccg ctcgctttaa tacctttacg tacaacttta
    26041 aaaatttcgt gcagcgcctc gtattcgctg accgcgccgc gatcgggatc gttcgggtca
    26101 atgccgttga aggcgagggc ttctggttgc aaatttgcgc cgacaaatgg ttcgacgtgg
    26161 aaatgtaatg tggtgtccgg catcagccag ccttgttcat ccattttcag ggtgatggcg
    26221 gcaatctcaa gcagcgcatc ggttttggcg ttaaatccgg ctgtttcaac atcgatcaca
    26281 acaggataaa aaccacgaaa acggtcgcac agaccggtaa gttgagcgtt atcggacatc
    26341 tgattctctt aatacggata aattgcagcg cgcattatga caaatattac ttgtcgatgc
    26401 agtaaagatg cgggcgcgat gagttcacgc ccggcaggag attagttgcc cagaccgcga
    26461 ccggcgtctt tctcttcgat taactcaatt ttgtaaccgt ccggatcttc cacaaacgcg
    26521 ataaccgtag taccgccttt taccggaccc gcttcacggg tcacgttacc cccgttttga
    26581 cggatttttt cgcacgcttc agcggcgtta tctacgctaa gcgcgatgtg accataagca
    26641 gtgccgagtt cgtatttatc cacgccccag ttgtaggtca gttcaatcac cgcttcttcg
    26701 gtttccgggc cgtagccaac aaacgccagt gagtatttgt attccgggtt ttcgctggta
    26761 cgcagcagtt tcatgcccag cactttggta taaaaatcga tggagcgttg cagatcgcca
    26821 acgcgcagca tggtatgaag aagacgcatc tttttatcct caaaatggct aacagattca
    26881 aaatgttgtt ttagtatagc ggcggcttta cgccgctcgc aatgttggat tacaacgtcg
    26941 ggtaatcggt atagccttcc gcgccgccac cgtagaaact ttcggcacgc tgtgggttaa
    27001 gctcagcttt gcgctgcaag cgggcgacca gatccgggtt cgcaatccag tcacgaccaa
    27061 atgccaccgc atcaattaac cctttgccga tcagcgtttc agctttttct actgtgtatg
    27121 cacctgcgcc gataatcgga ccgtggaaac gggcgcgtac tttttcgcgg aacgcatcag
    27181 tatacggttc accccccgcc caatctggtt ctgacatatg cagataagca atgccgcgtt
    27241 tacccagttg ttcaatcaga tacagtgcat cggcttcttc attcgggccg ttatccgtgt
    27301 tctggaaagt accgattggc gaaatgcgaa tgccaatgcg atcggcaccc cattcttcaa
    27361 tcccggcatc gaccacttcc agtaccagac gtgcgcgatt ttccacgctg ccgccgtact
    27421 gatcggtacg atggtttgaa gaaggagaaa ggaactgatg cagcaaataa ccgtgagcag
    27481 agtggagctc taccagatca aaaccggctt cacgcgcgtt agcaatggcc tgacggaaat
    27541 cattgacgat acctggaatc tcttccagtt caagcgcacg cggcatggat gtttcaacac
    27601 ggatcgcctg accattttca tcgcgcagag aagtacgtgt tcccgcgcta agtgctgaag
    27661 gcgctaccgg tgcctgaccg ccaggttgca ggctggcgtg agaaatgcgt ccggtgtgcc
    27721 acagctgcac ggccatatga ccattttcag catgaacgcc agcggtgatt tttttccatg
    27781 cggcaatttg ctccggacta tggatgccag gcgcacctgc atatcctttt gcctgggcag
    27841 aaatttgcgt ggcttcacta ataatcaaac cggcactggc acgttggcga tagtattccg
    27901 ccatcaacgg ggtaggaatg tcacccggtt caatactgcg cagacgcgtc agcggtgcca
    27961 taaaaatacg gtttgccgcc gtgatcgcgc ccactttcag tggggaatac agtttttcag
    28021 atgacataaa ggctcccggt cgtctggtga taaaaaatgc ctgctaaacg gcaggcgtcg
    28081 caataatgtt ttttacatgg gccagcgcgt tttccagtgg ctcgaaactg cgcgaaattt
    28141 tggcctgcag attcgcaccc agccatagtg cgtaaagcac ttgtgcctgt tgtagcggtt
    28201 cgccacaaaa ggttaaacaa tggttctcac ggccattttc cagcgcctga gagagcaggg
    28261 cgatcacgcc gcgagcacct ttatccatcg cgctgcgcat atcttctgac agatcgcaca
    28321 cttcggcaga gagttttact gtcaggcaac cactgatggt tccatgttgg caaaactggt
    28381 tcagtgtttg ctggtaataa gccagtatgc ggtcgcggta gttaccttcg ccggattgca
    28441 gcaactcagt cagtcgctgg tgatatgcgg cgtaatgacg ctcaagcatg gcaacgccaa
    28501 acgcttcttt agagcgaaag tagtgataga aggacccttt cggcacttca gcggttttta
    28561 gtaattcgct tagccccatc ccggtgaatc cacgttgcag gcaaagttgc tcgcccgtcg
    28621 ccaggagatg ttcgcgagta tcatgttcgg tgtgtttgtt catgcgttgg agtgtagtag
    28681 accagtcggt ctagtgcaag caaagttgct actttggaca ttaacagctt caataatggt
    28741 aataatctta tctgaggagg gcgaagtggc ggagcaatta gagttctttc ccgtccagag
    28801 cccgtgccgg ggaatttgcc agtctgacga acgcgggttt tgtcgcggct gttttcgtag
    28861 tcgtgatgag cgttttaact ggaataaaat gagcgatggc gaaaaacaag aggtgctgcg
    28921 cctttgtcgg cagcgattaa tgcgcaaatt acgcgcaaat aagccagcgt catcagacga
    28981 accggagcaa ccatcactct tttgaaccta atcttgcgta tactcctctc atatctgata
    29041 agaggaagcg attatggttc agcgtattac tattgcgccg caaggcccgg agttttcccg
    29101 ttttgtgatg ggctactggc gattgatgga ctggaatatg tccgcccgcc agctggtcag
    29161 ttttattgaa gagcatctgg atctcggcgt gaccaccgtg gaccatgctg atatttatgg
    29221 tggctatcag tgcgaagcgg cgtttggcga ggcactgaaa ctggcacctc acctgcgtga
    29281 acggatggaa atcgtcagta aatgcggtat cgcgacgacc gcgcgtgaag aaaacgtcat
    29341 tggtcattac atcactgacc gcgatcacat cattaagagc gccgaacagt cgctaattaa
    29401 tctcgcgacc gatcatctgg atctgctgtt aatccaccga ccagacccgt taatggatgc
    29461 cgatgaagtg gcggacgcgt tcaaacatct gcatcagagc ggcaaagtgc gtcattttgg
    29521 cgtatcgaac tttacgcctg cgcaatttgc cctgttgcaa tcacgtctgc cgtttaccct
    29581 tgccactaat caggtggaaa tatccccggt gcatcagccg ttactgctgg atggcacgct
    29641 cgaccaacta caacaactgc gtgttcgtcc gatggcgtgg tcctgccttg gtggtggtcg
    29701 tctgtttaat gatgattatt tccagccgct gcgtgatgaa ctggctgtgg tggcagagga
    29761 gttaaacgcg ggctcgattg aacaggtggt ttacgcctgg gtattacgtt taccatcgca
    29821 gccgctgcca attatcggtt caggtaaaat tgagcgcgta cgggcagctg tcgaagcaga
    29881 aacactgaaa atgacccgtc aacaatggtt tcgtatccgt aaagcggcac tggggtacga
    29941 cgtaccgtaa gcacttttag gaatagccgc cgttcaaaaa tgtgtcactg gtttacactt
    30001 attcaggaat gcacaatgaa cggaggtcct atgaaacgtt ttagtctggc tattctggcg
    30061 ctggttgttg caaccggcgc acaagctgcc agtgaaaaag tcgagatgaa cctcgtcacg
    30121 tcgcaagggg tagggcagtc aattggtagc gtcaccatta ctgaaaccga taaaggtctg
    30181 gagttttcgc ccgatctgaa agcattaccc cccggtgaac atggcttcca tattcatgcc
    30241 aaaggaagct gccagccagc caccaaagat ggcaaagcca gcgccgcgga atccgcaggc
    30301 gggcatcttg atccacaaaa taccggtaaa catgaagggc cagaaggtgc cgggcattta
    30361 ggcgatctgc ctgcactggt cgtcaataat gacggcaaag ctaccgatgc cgtcatcgcg
    30421 cctcgtctga aatcactgga tgaaatcaaa gacaaagcgc tgatggtcca cgttggcggc
    30481 gataatatgt ccgatcaacc taaaccgctg ggcggtggcg gtgaacgcta tgcctgtggt
    30541 gtaattaagt aattacgagg ccagcgtacc ttgcggtggt gcttgctcaa gttgcgaaag
    30601 cgagcagtac agccgccaga caattgccgc cagctcacgg gcggcaggtt gatgatgacg
    30661 ggcaaggctg ttgcaaatcc gctgtaattc ttcaagtgtg gccgccagtg atttttgctg
    30721 aacgccacgc tcactcatca ctccccgcaa cagtgaaata cagttatccc gtactcgcga
    30781 taacggatcg gaacgcgatt cccagtcgcg caattgccag acaacatgag aacagttcag
    30841 cagcactaca ccccagcgta ataaccaacg gcgagccagc gcatcctggc tgttactcaa
    30901 ctgactgacg tgatgataag tgagcgattc aaattcgctt tcactcagtg ttggatggcg
    30961 gcttagctga tcgacaaaat cccggcgcag cgcgcgaata tggcggcggc ttttacgagc
    31021 atccgatcct ggacgcagaa tggcgaacgc tagccaagcc agcgcaacgc caacgatttt
    31081 tgccagatta tcgttaagaa aatcagcaaa atcatacacc gggggattag tgacagcgat
    31141 aaacgaaccc ataaaaacaa tcagttgccc ccacagtgcg gcaaatttgg gcatctgcga
    31201 tttaagaagc tgcattgtcg ccagcagtgg aaagagaaac agtaaaaatt gccacagatc
    31261 gctaatctgg accatcaggc caaatttgac cacaaagctg aatagcgaaa gtaacaccag
    31321 cgtgcgcatc agcagtgaca acgacttaaa cggtgctgcg acggcggagt agagtacgca
    31381 gctaattgct gccagcgtta atgcattggc accgacatcc cattgcgatg caatactcca
    31441 tgcgccaatc atcatcaacg tacaaaatgt acgcagcccg ctccacatag cttcggcgtt
    31501 gtcggtatga cgagccagag cggatgtccg tgcgagtctg gcatgatcat ctacaccgct
    31561 ttgcagacga tgtaattcct gactactttg cagataaagg cggcaaaaat aacgtagtcg
    31621 ctgccagaag gcgacgtgcc ggtagtcggc gacgttggtc gggcgtagcg gggcgataat
    31681 acgtgcgacg gtgtaagcat ctgtttgcga actggcgagc gccgtcagca attgttcgag
    31741 aatttctcgc gtggcacctg gcggtgaggg ccagttgagc aacatacgtc gcaggctgga
    31801 gatgacactg gtcatacgta attgctggtg gagtagcgca ttaaggcgcg cgttttgctg
    31861 gcgaaaacga tagtggctcc agaaagcctg gatacgcagc aaattcatgg tcagtatctg
    31921 cccaatcacc ccctcatgtg ctgcacgaat ggcatcgttt gtttcaggct gccagagtaa
    31981 actggcatgt tccagtaatc gggcgtgcat gtttttcaat gcggttaaaa gggctgtagc
    32041 atcggaactg ctaggcagga tcatcatcat catgccgccg cacaaaatac cgactatcac
    32101 ctcgcaaacg cgcgcctgag cgatatccca cagctggctg gcctcagtaa tattaaccat
    32161 cggaaaggcg atgatggcag ccgtgtagcc cgccagttga aatgcatacg cgacgttatt
    32221 cgtgaagtgc gcacaggccc aggtacaaaa gccaagccac gccgacatgc tcaatagaaa
    32281 aaaccacggc tcattgagcg tatgcccggc aagaagcagt gccgcaatgg ctcccagtaa
    32341 actgccagcg atgcgtccga ggcttttgct gatgacaccg ccaacggtgg gaaagctaac
    32401 cactgcagcc gaggtcatcg cccaataggg ttcatccaga ttcaaataat aggcaaccgt
    32461 cagcgccaga cacatggcaa tggtattgcg taacgcataa cgccattgcg ccagcgtggc
    32521 cttgaaccag ggcaaattgc gcaaggacca tgacgatgcg ttcattattg acctacggca
    32581 atgctacagg tggtgcccga caccagcgtg acatcgcctg gaactttatc cagcgcaaag
    32641 cggacgggaa cgcgttgggc gagacgaacc caggggacgt taggtttcac atccggaata
    32701 aggcttgagt cactttcaac gctttgatca taaatcgccc gaccgatact ggaaacgtga
    32761 ccccgtaacg ttttgttatc actatagagg gtaatttgtg ctggtgcacc ttcgcggata
    32821 tggcgcaact tggtttcttc aaaataacca atgacgtaaa acgagtggct gtcgacaagg
    32881 gcaaacagcg gtttcccggt gtcagcgtaa tcgccgatgc gggtagtgag gttagtcacc
    32941 caaccactca ccggggcgcg aatttccgtt tgcgccagtt gccattgtgt ctgtttcagc
    33001 gtggcctgtg cggcattaac actggcctgc atcgctttaa cattgaggtt agcggtatcg
    33061 agttcttcgg cagaaataaa attttgtgag agatgacgac gacgattcgc ttcgttattg
    33121 gcttttgcga ggtcagattg cgctttcgcc agctgagcct gtgcgttcag ctcggcgatc
    33181 tgaaagggcg ttttatcgat cgtgagcaga agatcccccg cgttaaccag ttggttgtct
    33241 tttatattca gctcaacaat acgtcccgac acctgtgggg tgatagaaac cttttctgcg
    33301 cgtattttcc cgtcgcgtgt ccacggagat tgcatgtaat aattccacag ccaccatccg
    33361 gcaagaaccg caactatcgc tacaatgatt gttgagaaat acttaattgt tttaattgac
    33421 atagttacca cgcaatcagt attgccagag caaggcaaac gcaaatcgca aacagcgata
    33481 aatccattaa caagggatgc cagatgtcac cggcgtagat ccagccacga agcaggcggt
    33541 gtacgacaag ccagatgacg aatccaaacg cgaaggcttt gaaaaacgga ggaaaataga
    33601 cggacgcacc gaacaccaga tcttgtaagg gcaatcctgt tgcattgagc ataaacttca
    33661 caaagtagat tcctttacga ccgaatatat tgcgttagat taataaatat tctttaagtg
    33721 cgaaaaattt acgcgcaatt tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac
    33781 atttgtttta gcaatacaat tgctgcacac tattctaaaa gccgcataat atcttagcaa
    33841 gctaattata aggagatgaa attggaatcg ccactaggtt ctgatctggc acggttggtg
    33901 cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc tggagttaac acaaacccat
    33961 tgggttacgt tacacaatat ccatcagtta cctccagacc agtcgcaaat tcaactggca
    34021 aaagcgattg gcatcgagca gccatcactg gtccgtactc tggaccaact ggaagaaaaa
    34081 gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg
    34141 gaaaaggcag agccactgat cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa
    34201 atattacatg gcatctccgc agaggaactg gagcaactga ttacgctcat cgcaaaactt
    34261 gagcataata tcattgagtt acaggccaaa gggtgaaatg aagggggcgt gtggtcagta
    34321 acctgaccac acgcaaactt atttagcgcg gagaaacggt cacctgactg ccattgctgg
    34381 ccagtacgac acgttggccc ggagagaaac gagtgttgcc ttgtttctgt accaccatga
    34441 tggtattacc atcgtcttta cgaatttcca gctcgacacc ctgcgttttg ttcattgcac
    34501 tctgtacgcc ctgaccagct acgccacctg caacagcgcc tgctgcagta gccagagaac
    34561 gcccggttcc gccaccaaca gtattcccca ggaaaccacc aagaacagca ccgccaattg
    34621 caccgataac gttggaatca tcaccgccct gaatctgtac cggacgtacg ttaacgatgg
    34681 tgccatagct gacattctgt acttgtttcg cttcagaagc ggtataaaca tcccctgaca
    34741 gggtgtcgtt attaacacaa ccgacaagag acagacctac cattgaaaca accaatacgc
    34801 gtttaatcat tgaaaaatct cctgttcacc atgaaacgcc acgcaagcat ccctcatggt
    34861 caaagtatat ggcatatgtg aatccatggt tacatgttct accaaatatg agcaaatcat
    34921 aatgaaagta ggatcaacca attataaaca aaacattcat gatgattatt gatttgaaga
    34981 taatgtttta ctaccaacgg taaaaatcgg tcattccgca tggtattcac gaaaagttta
    35041 tgtttgagtg gtgcgcaaac gtcagattga aaaaggatga actatgaaat cgggccgctt
    35101 tattggcgtt atgtcaggca ccagccttga tggtgttgat gttgtgttgg cgacaataga
    35161 tgaacaccgg gtcgcacagc tggcaagctt gagctggccg atcccggtgt ctctgaaaca
    35221 ggctgtactg gatatttgcc agggccagca gcttacactt tcgcagtttg gacagcttga
    35281 tactcaactc gggcgacttt ttgctgatgc ggtcaatgcc ttgcttaagg aacaaaacct
    35341 gcaggcgaga gatatagttg cgatcggctg tcacggtcaa accgtctggc atgaaccgac
    35401 gggcgtggca ccacacactt tacagattgg cgataacaat caaattgtgg cacgcaccgg
    35461 aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac aaggcgcacc
    35521 gctggtacct gcgttccatc atgccttgct ggctcactca accgagcgac gaatggtgct
    35581 caatattggc ggcatcgcca atctgtcact gctcattcct gggcagccgg ttggcggcta
    35641 cgataccggt cctggtaaca tgctgatgga tgcctggatc tggcgtcagg ccggtaaacc
    35701 ttacgataaa gatgccgagt gggcacgggc gggtaaagtt attctcccac tgctgcaaaa
    35761 tatgctcagc gacccatatt tctcgcaacc tgcaccgaaa agcaccggac gcgaatactt
    35821 taactacggc tggctggagc gccatttacg ccattttccg ggtgttgatc cccgtgatgt
    35881 tcaggccaca ctggcagaac tcaccgccgt gaccatttct gaacaagttt tgttgagcgg
    35941 tggctgcgaa cgattgatgg tatgtggtgg aggtagtcgt aatccgctac tcatggcgcg
    36001 tctggcggca ttactgccag gcacagaagt caccaccacc gatgccgttg gcattagtgg
    36061 cgatgacatg gaagcattgg ctttcgcctg gcttgcctgg cggacgctgg cgggattacc
    36121 aggaaatctg ccttccgtca ctggcgcaag ccaggagacg gtactggggg ctattttccc
    36181 tgctaacccg tgatatcaga gttaactgaa tttttcttac cgcgtctgcc gttacacttg
    36241 gaacgttagg gagggcgtat tgccctccag accaggaaag tcttcgggaa atgtctatga
    36301 aaaaactgtt actgatctgt ttgccggtgc ttctcaccgg ctgtagcgcg ttcaatcaac
    36361 tggttgagca catgcaaacc gctacgctgg aataccagtg tgatgaaaaa ccgttgacgg
    36421 tcaaactgaa taatccgcgc caggaagtca gttttgttta cgataatcaa ctactgcatc
    36481 tcaaacaggg catttcagcg tctggtgcac gttacactga cggaatctat gttttctggt
    36541 cgaaaggcga tgaagcgact gtctataaac gcgaccgcat cgtattgaat aactgtcagt
    36601 tacaaaatcc acagcgttga gatttttcca ggggcggcgc acaatagcgt cacccactga
    36661 caatccgtaa agaaaaccat gtctgataac gacgaattgc agcaaatcgc gcatctgcgc
    36721 cgtgaataca ccaaaggcgg gttacgccgt cgcgatcttc ccgccgatcc attaaccctt
    36781 tttgaacgtt ggctctctca ggcgtgtgaa gccaaactgg cagaccctac cgcgatggtg
    36841 gtcgctaccg tggatgaaca tgctcagcct tatcaacgca tcgttttact caaacattac
    36901 gacgaaaaag gcatggtgtt ttacaccaac ctcggtagcc gtaaagcaca tcaacttgaa
    36961 aataatccgc gcgttagcct gctgttcccg tggcataccc ttgaacgcca ggtgatggtg
    37021 atcggtaaag cagaacgact ttcgactctc gaagtgatga aatattttca tagccgcccg
    37081 cgtgatagcc agattggtgc atgggtttcg aagcagtcca gtcgcatttc tgcccgcggt
    37141 atccttgaaa gtaaattcct ggagctgaag cagaagtttc aacagggcga agtgccattg
    37201 ccgagctttt ggggcggttt tcgcgtcagc cttgaacaga ttgagttctg gcagggcggt
    37261 gagcatcgcc tgcatgaccg ctttttgtac cagcgagaaa atgatgcgtg gaagattgat
    37321 cgtcttgcac cctgaaaaga tgcaaaaatc ttgctttaat cgctggtact cctgattctg
    37381 gcactttatt ctatgtctct ttcgcatctg gcgaaaagtc gtgtaccggc aaaggtgcag
    37441 tcgttatata catggagatt ttgatggcaa gcagtaactt gattaaacaa ttgcaagagc
    37501 gggggctggt agcccaggtg acggacgagg aagcgttagc agagcgactg gcgcaaggcc
    37561 cgatcgcgct ctattgcggc ttcgatccta ccgctgacag cttgcatttg gggcatcttg
    37621 ttccattgtt atgcctgaaa cgcttccagc aggcgggcca caagccggtt gcgctggtag
    37681 gcggcgcgac gggtctgatt ggcgacccga gcttcaaagc tgccgagcgt aagctgaaca
    37741 ccgaagaaac tgttcaggag tgggtggaca aaatccgtaa gcaggttgcc ccgttcctcg
    37801 atttcgactg tggagaaaac tctgctatcg cggcgaacaa ctatgactgg ttcggcaata
    37861 tgaatgtgct gaccttcctg cgcgatattg gcaaacactt ctccgttaac cagatgatca
    37921 acaaagaagc ggttaagcag cgtctcaacc gtgaagatca ggggatttcg ttcactgagt
    37981 tttcctacaa cctgttgcag ggttatgact tcgcctgtct gaacaaacag tacggtgtgg
    38041 tgctgcaaat tggtggttct gaccagtggg gtaacatcac ttctggtatc gacctgaccc
    38101 gtcgtctgca tcagaatcag gtgtttggcc tgaccgttcc gctgatcact aaagcagatg
    38161 gcaccaaatt tggtaaaact gaaggcggcg cagtctggtt ggatccgaag aaaaccagcc
    38221 cgtacaaatt ctaccagttc tggatcaaca ctgcggatgc cgacgtttac cgcttcctga
    38281 agttcttcac ctttatgagc attgaagaga tcaacgccct ggaagaagaa gataaaaaca
    38341 gcggtaaagc accgcgcgcc cagtatgtac tggcggagca ggtgactcgt ctggttcacg
    38401 gtgaagaagg tttacaggcg gcaaaacgta ttaccgaatg cctgttcagc ggttctttga
    38461 gtgcgctgag tgaagcggac ttcgaacagc tggcgcagga cggcgtaccg atggttgaga
    38521 tggaaaaggg cgcagacctg atgcaggcgc tggtcgattc tgaactgcaa ccttcccgtg
    38581 gtcaggcgcg taaaactatc gcctccaatg ccatcaccat taacggtgaa aaacagtccg
    38641 atcctgaata cttctttaaa gaagaagatc gtctgtttgg tcgttttacc ttactgcgtc
    38701 gcggcaaaaa gaattactgt ctgatttgct ggaaataatg cattaagtgg aaagggggag
    38761 tgagaaatca ctccccctgg tttttataca gggaacatga tgaaaaatat tctcgctatc
    38821 cagtctcacg ttgtttatgg tcatgcgggt aacagtgcgg cagagtttcc tatgcgccgc
    38881 ctgggcgcga acgtctggcc gctgaacacc gttcaatttt ctaatcacac ccaatacggc
    38941 aaatggactg gctgcgtgat gccgcccagc catttaaccg aaattgtgca aggcattgcc
    39001 gccattgata aattacacac ctgtgatgcc gtgttaagtg gctatctggg atcggcggag
    39061 cagggtgaac atatcctcgg tatcgtccgt caggtgaaag ccgcgaatcc gcaggcgaaa
    39121 tatttttgcg atccggtaat gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc
    39181 gcagagtttc atgtgcggca cggtttgcct gccagcgata tcattgcgcc gaatctggtt
    39241 gagctggaaa tactctgtga gcatgcggta aataacgtcg aagaagcggt tctggcagcg
    39301 cgcgaactca ttgcgcaagg gccacaaatt gtgttggtta aacacctggc gcgagctggc
    39361 tacagccgtg accgttttga aatgctgctg gtcaccgccg atgaagcctg gcatatcagc
    39421 cgtccgctgg tggattttgg tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt
    39481 ttactgctgg tgaaactgct tcagggggca acgctgcagg aggcgctgga acatgtgacc
    39541 gctgcagtct acgaaatcat ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg
    39601 gtggctgctc aggatcgtat tgccaaacca gaacattact tcagcgcaac aaagctctga
    39661 aatatctcat ttcggcccac aacgacctgt gggccgatgg atattacttt aagccttccg
    39721 ctgacagcgc gtcttgtact tccggacgtt cagccatacg ttgcataaat gctgcaatgt
    39781 gctctaaccc ttccagattc agtttcaccg cgtatgccca gcgcagaacc gtaaacagat
    39841 aggcatcagc aattgtaaat ctttgcccgc agatccagtg ctcatccttc agtgcctcgt
    39901 tcacatattg cagcttcttc tccagctgcg cgcgaactgt cggtttgtac tcttccggtg
    39961 tatcagggcg aaacagaggt gtgaaacctt tatgcagctc ggtggcgatg taattcagcc
    40021 attcgatggt tttatagcgg gaaatactgt ttaccggtgc cagcaactgg cggtcgggga
    40081 cgctgtcggc aagatactgc ataatcgcta cgccttccgt cagcaaagta ccgtcatcca
    40141 gcagcactgc aggcacctgc cccttagggt taacgacaaa gtaatcgtca ccgttttcga
    40201 gacgtttttt cattaaatcc acactgacga gggtaaaatc ctttccgctc tcacgcaggg
    40261 tgatatggga agcgagagag caggcacccg gtttgtagaa caatttcatc ggtaactcct
    40321 tttgtctgtg gtttaaagta tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa
    40381 aagttagcgg cttgtaaaag agtttccctg aattttacgc tacagcagct ttcgccgctt
    40441 tgtctgcagc gtcatcctgc gtcatacggt gcagtttagg cgcggtcagc agcatcagta
    40501 ctgcaataat ggcagtagcg acaccaatct gcaagaatac gcgaccataa acttccagtg
    40561 acatcagcgg gtcggtaacg ttatccggca cagccatcat acccgcaaca taaccaccaa
    40621 tcaggtttgc accggcagtg gtcaggaacc agctacccat aatgaagccc atcagacgct
    40681 gcggaacgag ttgtgcaacc attgccagac ccagaccaga gatcatcagt tccccgatgc
    40741 tctgcaggcc atagcttgcg accagccagc ttacagacac gataccagcg tcagacgcga
    40801 atttcgcacc cagcggcaga atcaggaacg caccagaaca catcaccatg ccgattgcaa
    40861 acttggttgg catcggcagg gtatcgccca tcttgttata gatagcggcc agaatcggac
    40921 taccgatgat gatccagaac gggttcagtg cctgatactg ctcaggttct acggccagac
    40981 ccagaatgga gtgctcaacg ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc
    41041 tgtacagcac gaagaagata atggcttcga gcatcaggat gaaggcaacg atcattttac
    41101 gacgcgcagc acctttcatc gcgaaggctt ctttaccgaa gataaccacg ataccgaagg
    41161 caacaacgcc cagcgccata cgcgcaactt cctgattgtg cagcagccag gtggcgatag
    41221 cgatcagtgc cacaacacca ataatggtca gcagcaggtt acggtagttg ataggctcga
    41281 agtctggttt tgaaccgtac tgtttaaccc agcgttggca gaaggcgaag ttaacgatag
    41341 tgatcagcag gcctacaacg ctcaacgcaa acgcaacact ccagccgtat ttcgcggcca
    41401 gccacggcgt agcaatcata gagaagaaag agccgatgtt gacggacatg tagtacatgg
    41461 tgaatgcacc gtccagacgc gggtcgtttt tctcatagca tgtagaaagc agagaagacg
    41521 ggttagcttt aaacaggccg ttaccgaccg caatagccgc catacccata taaacgatac
    41581 cggcgtcgtg accagaccag gcaaccagag cataaccaat cgccagcaca atagcgccga
    41641 gcataattac gcgtttagta cccagtacct tgtcacctaa ccagccgccg atagcgacca
    41701 gaccataaac cagggcacta aaggaagaga aaagggtgat tgaatccgct tcagacatac
    41761 ccagttgttt aaccaggtaa acagccataa ttccttgtag gccgtaataa ccaaaacgtt
    41821 cccataactc aatcgagaag atgagataga acgccttcgg ttgtttgaaa gcgttcaaac
    41881 tgacgctttc agttggtttt tggtttgcag tggacacttt tacctctttt tttacatccc
    41941 atattaacgg gggtgctttc tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt
    42001 gggaggggaa acggcaggta atgttcacta tcttgcactt tcagacaagt cctttgtaat
    42061 aatctgttac atataactga ggcggggaat tcaaccattc cgggaaatgt tatccagcgt
    42121 taaatttttc aaaaaacgta acggcagatt ttttcactgc actgagaggg gggtgatggc
    42181 ttaacggcga tatgttctga tatttagcgt ttttactagt gagatagtcc agtttctgaa
    42241 aaatagccag tgtaatgttt tgtaggtcaa tatttgttgc gtgtgtaaat ttaaatgatt
    42301 ttaacctgat tcttgcaatg tttttacatt gcgacagcat cgtgatcttg atcacgaatg
    42361 aatagaaaat ttcgctatta aaaaaacaat taacctgcat ttttggttat ccgcaaacgg
    42421 attacctgat gataacgcta agcaatggca tcattcacgt taaaaagaag ttttaaaaga
    42481 ggataaagaa aggttatcaa tggggtaatc ggtgttaccc cttttcttca gatgtcaact
    42541 ttctctttgt attcacaaag atcttcaata atacaagagc cacagcgggg cttgcgggca
    42601 atgcaggtat aacgcccgtg caggatcaac caatggtggc agtcgacttt aaactctgcc
    42661 ggaaccactt tcagtagctt ttcttctacc tgttcgacgt ttttccccgg cgcaaattga
    42721 gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca
    42781 gtgtttaata cgacgttggc tgttttacga cctacgccgg gcagggcttc aagcgcagca
    42841 cgatcttccg gaacctcgcc attatgctgc tccagcaaga tacggcaggt tttgatgata
    42901 ttttctgctt tgctgttata aagcccaatc gttttgatat aggttttcac cccttcaacg
    42961 cccagttcaa gcatcgctgc aggcgtattc gccaccgggt agagtttcgc cgtcgcctta
    43021 ttaacactga catcggtcgc ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc
    43081 gaactgaaat taagctcggt ggtgggatga ggattgttct cacgcaggcg agtgaggatc
    43141 tccaggcgtt ttgctttgtt catcagacat tccctgtttc accgtttggc aatgcacgtt
    43201 cggcagctgc ttcagtacgg cgctttttca ttctttcatc aatcaggtat tttcctgcca
    43261 gcatcagtcc caggccaata aatgcacctg gaggcagcat cgccagcagg aaaggggagt
    43321 cggtgtggaa aatttccacg cgtaatactt ttgcccagct acctaacagc gcatctgcac
    43381 cgtcaaacaa tgtgccattg ccgataattt cgcgtagtga acccagcacg aacatggcgc
    43441 aggttgcgcc cataccaatt gaaaagccgt ccagtgccga aagcgccgga ccttttttgg
    43501 cggcgaaggc ttcagcgcgg cccacaacga tacagttagt gacaatcagc ggaataaaaa
    43561 tccctaatga ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt acagcgctga
    43621 ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat ctcggctggc gtccagtgac
    43681 gcagcgtcga aatggtcagg ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac
    43741 ccagagcgtt agtggcagtg gacgtgaccg ccaacagagg acaaaggccg agcaactgga
    43801 ccagcgcaga gttgtttttc cacaacccct gaacaataac gtctttaatt tcgctcacgc
    43861 tttattctcc acaggcagga agttgagaaa gttgtgccgg taacgtctga gcgtacaatc
    43921 cggcgcgttt taccgcatta accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt
    43981 cgaaatcacc accatctttc ttcaccgccc agtgcgcatc atctgcacca ctgatttttt
    44041 tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc gattttatcg ccaagccctg
    44101 gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt gccgttaaaa tcggctccca
    44161 ccagcagctg aatcgcaccg gaatagccat ctggcgcggt tgcttccaga acggcggcta
    44221 ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc acctttacct aactctggcg
    44281 cagttaccag atagcaactc tgtgccagcg cattgttata gcgttcggct ggcagcacct
    44341 gatcaaataa tgccttttgt tgcagactgg cctgttcagc aatcgtcgtt ttggtcatct
    44401 ggttgatggc cgcagttaac cctgttgaac ccgctgcaaa tagcgctaac gtaatgccgt
    44461 gttttcggat agttttcagc atggtttacc ctttgcgatg gccgtagacg cgcggacgcg
    44521 tgtagtaatc gatcagagga accgtgatgt tcgccagcag gacggcaaaa gccacgccgt
    44581 caggatagcc gccgaaactg cggatcaacc agactaataa gcccgcaagc gcgccgaaaa
    44641 taagacgacc acgattggtc gtagaagcgg taaccgggtc agtcaaaata aagaatgcgc
    44701 cgagcatggt cgcaccagac agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga
    44761 acaaccagcc caacgttgcg cataacgcca gcgttactaa gaagctgagg ggaatatgcc
    44821 agcgaatcgc tttctgccat agcaaccaca ggccgccagc cagccaggcg agatttaccc
    44881 attgccaacc agcgcccgcc agaataccgc tgtagatcgg atattgcata atctgttcaa
    44941 ccgaatgacc ggcacggaca gaggttttaa atgtatccag cggtgtcgcc tgactaatgc
    45001 catcaatacc taagcgtagt gtgttcatat caccaccact ggcggtatgc ccgctgaaaa
    45061 taacctgtat ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc
    45121 agctggtcat ctgcaccggg aaggagatca gtaagaccac ataaccaatc attgctggat
    45181 taaacgggtt ttgccccaga ccgccataca actgtttagc gatgatcacc gcaaacaccg
    45241 tacccagcac gaccatccac catggcgcga gggggggaat acttaccgcc agcaataagc
    45301 ctgtcagcaa tgctgagtta tctttcaacg ttgcggctac cgactgcttg cgtagtttga
    45361 gtacgagagc ttcggctaac agagcactaa ccgatgccaa caggatctga acgagagtac
    45421 cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca
    45481 gcataatgcg cgatgtctgg cgctggttat gggtataagg ggagctagct attctgaata
    45541 ccatttagtc ctcgtttaca accttctgct gggcggcttt tttggcctga gcgcgggcaa
    45601 tagccgcggc aactgccgct ttgcgcggat caacctgttc ttctggttct gcgttagtct
    45661 gttgctgttc cagcttgcgc gctttggcac gggcaatagc ggcttcgacg gcggctttgc
    45721 gcggatcaac ctgttcttct ggttccgcat tagcctgttg ctgttccagt ttgcgcgctt
    45781 tggctcgggc aatagcggct tcgacggcgg ctttgcgcgg atcaacctgt tcttctggtt
    45841 ccgcattaga ctgttgctgt tccagcttgc gcgctttggc acgggcaata gcggcttcga
    45901 cggcggcttt gcgcggatcg acctgttctt ctggttccgc attagcctgt tgctgttcca
    45961 gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgg ggatcgacct
    46021 gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgcgctttg gcgcgggcga
    46081 tagctgcttc aacggcagtt ttacgtggat cagcaacggt tgctgcgtcg ttagtttgct
    46141 gcagttctgc ctgtttcgct ctggcttgcg ctttacgggc ttcccgtgct gcaataattg
    46201 cactgttatc cgggcgttcg cccgctttaa tcacaatagg ctgtgtagcc tgggcctgtt
    46261 tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt atctttggct gcaggttgaa
    46321 cggctgcgct cttatgtcgt tcaaggcgag ccgctttttc gcgctccaga cgagcctggc
    46381 gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc ttcctgacga atagccgcaa
    46441 tttcagcttt ttcctgacgg aaatattgca ccaggggaat attgctcggg caaacccacg
    46501 cgcaagcccc acattcaatg caatcagcaa tgttatgcgt ggtagcttta tcgtgttgct
    46561 gacctttgct gaaccagtac aactgttgcg gcaaaagatc cgcagggcag gcgtcagcac
    46621 aggcgctaca ccggatgcag ctttgttctt cctgtggttc gccaagttca ttggcagagg
    46681 gagccaacag acagttggta atctttacga ccgggacatc cagccatggc aaggtaaagc
    46741 ccattagcgg gccacccata atcaccattt gatcggcaga ggggcagaat ccggcatcat
    46801 tcaataaatg acgcactggc gtccccagcc gtgcccagac gttgcccggg cgagcgattg
    46861 cttcgccagt cagggttaca acacgctcgg taatcggctc gccatcaata acggcacgtt
    46921 tcactgcata agcagtgccg acgttttgca ttaatacgcc gatatcggat gaacgcccgc
    46981 catgtggaac ctgcttcccg gtcagaatgt aggttaattg tttagcaccg ccagaaggat
    47041 atttggttgg aatcacccgc agagaaatat cgttagagtc cgccagcacc gcgcgcagca
    47101 tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag aatttcgcgt ggctgcagaa
    47161 tatgcgcaag aatgcgaata ccctctacga cctgagccgc gcaatcctgc atcaaacggt
    47221 catcggcggt aatgtacggc tcgcactcag ccgcgttgat aatcaacgtt tcaatcttat
    47281 ctccgccacc ctgcaattta acgcctgtcg ggaatcctgc accgcccagc ccggctacac
    47341 caaactgatg tatgcgctcg attaactctt cgcgactgcg agtgcgataa tcggcccagc
    47401 cgtcgcgcgg gatccagcag tcttcaccat cggcatcaat aatcacgctt aattcagcta
    47461 aagctgaagg atgagccgta gagtggggcg caatagccgt aacggtaccc gaggtgggcg
    47521 cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg ctggccgcgc aatactttat
    47581 cgccgacgct aacgcacaac tcaccttcag cgccaatatg ctgtttcagt ggaataacaa
    47641 aacgctgcgc cagtggtacc tggcgcaggg gtgtaccgtt ggactgggtt ttcatctccg
    47701 gtggatggat gccgccgttg aaatcccaga ttttattttt tctgaatgca gagaataact
    47761 taagcatggt gttccacggg aatgatacgc acgggaatgg tgttcagatc ccatttccag
    47821 gagtcaggtg tttctgcgac cggttgcaac gagatgcagt gcgtcgggca cggatcaaca
    47881 cataaattgc agcccgtaca gagatcactc attaccgtat gcatggcacg ggtagcgcca
    47941 acgatggcgt ctaccggaca agcctgaata catttagtgc agccaataca gttattttca
    48001 tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg cttcgccatc cagcggctgc
    48061 ggctcgacat taagcaactc ggcaattttt agcatcacag cttcgccacc tggggcgcaa
    48121 cggttgattt tttcaccgtt acagctgatg gcttccgcgt aggggcgaca gccgggataa
    48181 ccgcactgac cacactggct ctgcggtaag atttcgtcaa ttttctcaac gaccggatcg
    48241 tcttccaccg caaaacggcg ggaggcataa cccagaatgg cgccaaacgc caggcccagc
    48301 aggctcacgg cggcaacggc aatccagata gcattcatta caacttcacc aaaccactaa
    48361 agcccataaa ggccagagac ataagacctg cggtaattaa cgcaatggca ttaccgcgaa
    48421 aaggtgccgg gacatcagcc acagcaaggc gttcgcggat ggcggcgaag agcaccatca
    48481 ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc cgactgcaag aaattgtgcc
    48541 cgagattgat attcagcaac gccacgccga gcactgcaca gttggtggtg ataagcggca
    48601 aaaaaatccc cagcagacgg taaagcaccg ggctggtttt gcgcaccacc atctcggtga
    48661 actgcacgac cacagcaatc accagaataa atgccagggt gcgcaggtaa atcagattaa
    48721 gtgggatcaa aatccacgta tcgataagcc aggcgcaaat agacgccagc gtcatcacaa
    48781 acgttgttgc cagccccatg cccatcgcgg tttccagttt tttggaaacc cccataaacg
    48841 gacagagacc gagaaacttg accagtacaa agttattgac cagtacagtt ccgacaaaca
    48901 gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa gccgcctatt atccgttaat
    48961 ccagagcagg cgacaacagg ttaactgtaa ggttattacg gattcacaaa cgtcgctttt
    49021 acccgcgacg agcgtttgaa ataggggacg atcaatgcgg ttgccaacaa agtaaacagc
    49081 aactggcgaa cggctatgcc atcttccacg ggtgaaaaag cgaatgcttt tactgccagc
    49141 aatacggaaa taagcagcca gataatatag tgtttaggaa cacaacgccg acgtttaaag
    49201 aaagcgatgg tcaaccacag tgtgtaatac cacatggcga tagcggtaat aaaagaaacg
    49261 ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg tttgaaatgt ttgcggagaa
    49321 gataacgcag cggtatacag caacaacgcc agcgtcgtac tcaacagagc gactaaaagc
    49381 caggccaacg ggccaagcaa ccagcctcca atacgttgtg gcgttgtggt ggtcataggt
    49441 tctccagaat agcgacgcaa acaggcaaat ctaattttag gggcgttatt ataaggcttt
    49501 tataaccgat cgctactttc tttattggac atagtgccag acggacttcg gtacctggcc
    49561 gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga cggtgatcgg cagcacgata
    49621 catattgatc agttcctgat cgtccgtcag ggtgtaatta agatggtcgt agagtttttc
    49681 cagactttcg agtgaactga ttttgcgaaa ttttaataag tagtcctgaa cagtcataaa
    49741 aggtccataa aataattaat accaaagggg taggtattat tagtgatgat aaaatttcac
    49801 caaaagtgaa cgcttgtgtt attacaataa taagaatgcg ctgatggcaa atattcaggg
    49861 atgaaaaact gctctgaaaa gagtaacgcg ctttactatc cctgacaatc accaacaaca
    49921 tcgaacaaga taataaattc ctggtttaat atccgacaag tgaaaacatg cacccggacg
    49981 ggcagcatgt cgctccacaa gtgcagagct tacttgtgtt gtaccgaagc actctgttca
    50041 ggtggctgat agttgtcaat gtgactcgcc acgccaagaa gaatgactga aacgacaaga
    50101 acgatccaac ctgttaattc aataagacga ttcattacag cccacatctc tcttgattga
    50161 tccattaact tcagggggta aatgttactt agcaataata gctccagcaa gatttttact
    50221 gaggtttttg cgatatttag cttttgtcgt tggaaaattc gctatttttt tgacttaagt
    50281 taaacaacat cccttattgc tggcaggtta ttaaactgtt gagcgtgggt aaaggatagt
    50341 gtcaaatagc catcatactt caatgagagg caatgacatg agcgacaaca tccgtgttgg
    50401 gttgattggg tatggttatg cgagcaaaac cttccatgcg cccctgattg cgggcacgcc
    50461 cgggctggaa ctggcggtaa tctccagcag tgatgaaaca aaagtaaaag ccgactggcc
    50521 aacggttacg gttgtctctg agccgaagca tctgtttaac gatcccaaca tagacctgat
    50581 tgtcattcct acacccaacg atacccattt cccgttagcc aaagcggcgc ttgaggcggg
    50641 taaacatgtg gtcgttgata aaccctttac cgtgacactg tcacaagcgc gagagctgga
    50701 tgcgctggca aaaagcctgg ggcgtgtgct gtctgtattc cataaccgtc gctgggatag
    50761 cgatttcttg acgctaaaag gtttactcgc ggaaggcgtg ctgggtgaag ttgcttactt
    50821 tgagtctcat tttgaccgct tccgtccgca ggtgcgcgat cgttggcgtg aacagggcgg
    50881 cccaggcagc ggtatctggt acgatttagc accacatctt cttgatcagg ccattacgct
    50941 ttttggttta ccggtcagca tgacggtaga tttggcacag ttacggcccg gagcgcagtc
    51001 gaccgattat ttccacgcca tcttgtccta tccacagcgg cgagtcattt tacacggtac
    51061 catgctggca gccgctgagt cagcacggta tatcgtgcat ggatcccgag gcagttatgt
    51121 gaaatatggc ctcgatccac aggaagaacg tctgaaaaat ggcgagcgtc taccgcagga
    51181 agactggggc tacgatatgc gtgatggcgt acttacccgc gtggaaggtg aggaacgtgt
    51241 cgaagaaacg ctgttgacgg tgcctgggaa ttatccggct tactatgcgg ctattcgtga
    51301 tgcgttaaat ggcgatggtg aaaatccggt tccggcaagc caggcaatcc aggtaatgga
    51361 gttgattgag ctgggcatcg aatccgccaa acatcgcgcg actttgtgcc ttgcatgaaa
    51421 aatggatggt gcaatttgca ccatccattt tgttacttcg cggcgacttt ttctcgcagt
    51481 gcgcgttttt cctcagcgct gaggaaagcc atttccagac cattaatctg tgcctggcgg
    51541 atttgctcgc gggataaccc agcagctggc gcggcaacgg tatattcgtg aatgatatcc
    51601 actccctgta cgccgggatc gtcagtgtta atgctggcac gaatgccatg ctcaaggaac
    51661 gttttcagcg gatgtgcagc cagctctgct acggtgctgg tctgaatatt ggaggtcaga
    51721 caggattcaa taccaatttg ttgctcggcg agaaaatcca tcagcgcccg atcttcaatg
    51781 gcttttacgc catgtccaat acgctccgca cccagttcac gaatcgcctg ccagatgctt
    51841 tccggcccgg cagcttcgcc tgcatggacg gtaatatgcc agcccgcatc acgcgcgcgg
    51901 ttgaagtgag aaaggaacag acttcccggg aaaccaagtt catcaccggc taaatcaagt
    51961 gcggtaatct ggtcacggtg ggctaaaaag gcctccagct cttgctgaca ggcggcttcg
    52021 ccgaaggtcc ggctcataat gccgataagc ttcgcctgca caccaaaggt gcggcaacct
    52081 tcacgtacgc catcgatcac cgcttcgaca acacccgcta caggcagctg atgtgccatt
    52141 gccatgtagc ctggtgaaaa acgcagctcg acatagtgca ggccgtgacg ggctgcatct
    52201 tcaatgtttt caaatgccac gcggcgacag gcatcaagag aggcgagaac tttaacgccc
    52261 cagtcaagtt tggtcagaaa gctcaccaga tcgggttcgt tggcaatgac ctgaacgtgg
    52321 ggaatcagtg tttccaggga ttgtgcagga agcgagatat tatactggcg gccaagttca
    52381 agaatggtct ggggacgaat gttgccatca aggtggcgat ggatatcagt taatggcagg
    52441 gtggtatcaa tcatggtcgc actctttttc tgaatataaa agtgcgcacc attataaaaa
    52501 caaagcaggg taaaaatcca cgattgcgca acgatttttt acgttgcgca attggggtta
    52561 gcgaacagcg cggatggcgt ttattaatcc agccacacct ttttccagtt tcgaacgtgg
    52621 gcagccggca ttgagacgga caaaaccacg accttcttca ccataggtat accccggcat
    52681 gatcgcgact ttttcttgct cgataagcgc tttttgcaac gcgttgtcgt caatattcaa
    52741 cggacgtaga tcaagccagg ccagataagt ggattgcggg atctgccagt tgagttcagg
    52801 aaacgcggcg ttcattttat ctgagatata cgtcaggtta tctttcagat agacgcgtaa
    52861 ggcatccagc cacggtgcac cttgctgata ggcggcgata tgggcagtta acgccagtac
    52921 cgaaggggaa gaaagcccat cacgtccttt cagtgccgat aaataggcat cacggctgct
    52981 gctattttct ataatcccgt aagcaccggt cagcgcggga atattgaaac ttttcgagcc
    53041 cgacgtcagc aacgcccagt ctccccgcgc cacattactc cagggaatat gcggctgctc
    53101 gccccagacc atatccatat ggatttcatc ggaaataacc cgcacaccat gacgctcgca
    53161 caggtcagcc atgatctcca gctcatcgca cgtccacact ttcccggtag gattctgtgg
    53221 gctacacagg agcataattt tacattctgg tttcgccaac acggcttcca gcttgcccat
    53281 atcgcaaaac cagtcgtcag cctgcttctc taaagcaacg ggcattactg tgcgctggtt
    53341 accttcaatg gccttgtaaa atgcgtcata ggcgggcgtg tggatcacca cgccttcacc
    53401 tgtttcagac cactgacgaa tcagttctga aaccatatag atgacagaag ggccatacac
    53461 caccgtctgc gtatcaattg cggtgtaatg ctgggtggaa aaccagtggg caatagccgc
    53521 gagaaactca tcgtttttcc agcggctgta gccaaatacg ccgtgcatca ggcgctgatt
    53581 cagcgcctcg ataatgcagg gggcagtggc aaaatccatg tctgaaatcg tgaacggtaa
    53641 caggtcagca gtgccgaaac ggtcagcgac ataatcccac tgtgtacacc atgtgccatg
    53701 acgatccacg acctttgaaa aatcgaacat atcttatcct tatgcctgga cagtatgcat
    53761 cagaccggcc atttcatctt ttactgactg aacttgtggc ccgataacaa cctgcaggtt
    53821 atgttgatta agttgtacta cgccaattgc ccgattgtcc ttcagtgcct gcacattaac
    53881 aagcgacata tctttcacag acaaacgcag acgggtaatg cagttatcga ggctgacaat
    53941 attgtcggca ccgcctaatg cttcgaggat tgcaggaacg ttgtaacctg atttacccgg
    54001 cgcaccggca acggcttttt cgattgagct ggcaacttcg ctatcgcgcc ccggggtttt
    54061 cagattgaag cgggtgatag cgaaacggaa gatgacgtag taaacgacaa accagattgc
    54121 cgccaccact ggcaccatgt accacttggt tgacagacca tgcaaaatac cgaacaccac
    54181 gaagtcgatg atattgccgt cggtattacc gatggtgacg ccgagcacag acatgacggt
    54241 gaagccgagg ccggttaaca gcgcgtggat gacatacaga actggcgcta cgaacaggaa
    54301 caggaattcc agcggttcgg tagtgccgcc aacgacgcag gcgatcaggc cagaaatcag
    54361 cagaccttta attttatggc gattttccgg gcgcgcgcag tgatacatag ctaacgctgc
    54421 acccggcaga ccgccgagaa acgcaggcat tttaccttgc gaaaggaaac gcgtggcgct
    54481 ttcagaaaaa ccgtgagtgg tcgggcaact caattgcgcc tggaagatgg tcagtgcgcc
    54541 gctgacggtt tgaccgcaga cttcctgcgt gccgcctgcg tcggtaaagc gaattaatgc
    54601 taccagaatg tgatgcagac caaacggcaa cagcagacgt tcaccggtac caaacagcat
    54661 cggtccgaaa tcacccgcgc tgtttatcat atggcccaag ccgctaatac ccatggcgaa
    54721 aatcggccag actaatggaa tcaccaggcc gacaaggccc atcaccagcg aggagataat
    54781 tggtacgaag cgcgtaccgc cgaagaatgc cagcgcatcc ggcaggcgga tattatggaa
    54841 acgctcatgc agcatccaga cgataatacc ggcgatcacc gcaccgagga tcccggtatc
    54901 gatcgactgg atcccaagaa tgctctggat gttattggct ttcagaaccg cggcatccgt
    54961 ggttggcaga atgcctttat tggtcaacca gaagtttacc gcgaggttca ttaccgcata
    55021 accgacgaag ccagcgaatg ccgctacgcc tttattttcg cgtgccaggc ccagcgggat
    55081 ggcgatacag aacatgacag gcaggaaact aaaagcaaac gagccaatct tactcatcca
    55141 ggtaaagata gcctgcaaca cggggttgcc caggaccggg atcagggtta tgacatcatg
    55201 gctgctaaga gaactaccaa tgccgagcat aatgccgcag aacgacaata atgccacggg
    55261 taacatgaag gttttgccta actgctggaa gaactcccac agcgtgactt tcggtgctgt
    55321 tttcgccgtc ataaaacgac tcctcgtaga gaacaatctg gattcagtaa attgcgcgag
    55381 aagataaaac gttttatcaa attttagtga ggcataaatc acattacgca acgataatag
    55441 cgggtataag ataaataaaa ggtaaaacgt tttatctgtc acataatcag ggagtaggtc
    55501 atctgcatgg ctaccgccaa aaaaataacc attcatgatg ttgcgctggc tgcgggcgtg
    55561 tcggtaagta ccgtttcgct ggtgcttagt ggcaaagggc gaatctctac cgccacagga
    55621 gaacgcgtta acgccgccat tgaagagctg ggatttgtgc gcaatcgcca ggcgtcggcg
    55681 ctgcgcggcg ggcaaagcgg cgtcattggt ttgatcgtcc gtgatttatc tgcgccgttt
    55741 tacgccgaat tgacggccgg attgacggaa gctctggaag cgcagggacg gatggttttt
    55801 ttgcttcacg gcggtaaaga cggcgagcag ctggcacagc ggttttcact gttactgaat
    55861 cagggtgtgg atggtgtggt aattgccggg gctgcaggaa gcagcgatga cctgcgacgg
    55921 atggcagaag aaaaagctat cccggtgatt ttcgcttccc gtgccagtta tcttgatgat
    55981 gttgatacgg ttcgcccgga caacatgcag gctgcacagt tgttgacgga gcatctcatt
    56041 cgcaatgggc atcagcggat cgcctggctg ggagggcaaa gttcctcatt aacccgtgca
    56101 gaacgggtgg ggggctattg tgcaactcta ctaaaatttg gcctgccgtt tcacagcgat
    56161 tgggtgctgg agtgcacatc cagccagaag caagccgcgg aagctatcac ggcgctttta
    56221 cgtcataacc cgaccatcag tgctgtggtt tgctataacg aaactattgc gatgggggca
    56281 tggtttggtt tgctcaaagc agggcggcaa agcggggaaa gcggagtcga tcgttacttt
    56341 gagcaacaag tttcgctggc ggcatttacc gatgcgacac caaccacact tgatgatata
    56401 cccgttacct gggccagcac gcctgcgcgg gaacttggta ccacacttgc ggatcgcatg
    56461 atgcaaaaaa tcacccatga agagacgcat tcacgcaatc ttattattcc cgcccggctc
    56521 attgcggcga aataatcctc tctttatctg ctatacctgg tagtgtcctt tcctcaaggt
    56581 taatgataaa aaatgtagca cacaggcggt gccttttatt tttactgaag ttcacaggag
    56641 gtttatgtgt ttaattctga caacctgaga ctcgacggaa aatgcgccat catcacaggt
    56701 gcgggtgcag gtattggtaa agaaatcgcc attacattcg cgacagctgg cgcatctgtg
    56761 gtggtcagtg atattaacgc cgacgcagct aaccatgttg tagacgaaat tcaacaactg
    56821 ggtggtcagg catttgcctg ccgttgtgat attacttccg aacaggaact ctctgcactg
    56881 gcagactttg ccgtcagtaa gctgggtaaa gttgatatcc tggttaacaa cgccggtggc
    56941 ggtggtccta aaccgtttga tatgccaatg gcagattttc gccgtgctta tgaactgaat
    57001 gtgttttctt ttttccatct gtcacaactt gttgcgccag aaatggaaaa aaatggcggt
    57061 ggcgttattc tgaccatcac ttctatggcg gcagaaaata aaaatataaa catgacttcc
    57121 tatgcatcat ctaaagctgc ggccagtcat ctggtcagaa atatggcatt tgacctgggt
    57181 gaaaaaaata ttcgggtgaa tggcattgcg ccgggggcaa tattaaccga tgccctgaaa
    57241 tccgttatta caccagaaat tgaacaaaaa atgttacagc acacgccgat cagacgtctg
    57301 ggccaaccgc aagatattgc taacgcggcg ctgttccttt gctcgcctgc agccagctgg
    57361 gtaagcggac aaattctcac cgtctccggt ggtggggtac aggagctcaa ttaatacact
    57421 aacgaaccgg taaacagccg tatgtgttgt ttaccgggat agcgtgtgca attcatcaac
    57481 gtctctgcta aacaactggc agccaaatca cggctattgg ttaaccaata gctggatgaa
    57541 aagcatacga atagagtgtg ccttcgcact attcaacagc aatgataggc gctcacctga
    57601 caacgcggta aactagttat tcacgctaac tataatggtt taatgatgga taacatgcag
    57661 actgaagcac aaccgacacg gacccggatc ctcaatgctg ccagagagat tttttcagaa
    57721 aatggatttc acagtgcctc gatgaaagcc atctgtaaat cctgcgccat tagtcccggg
    57781 acgctctatc accatttcat ctccaaagaa gccttgattc aggcgattat cttacaggac
    57841 caggagaggg cgctggcccg tttccgggaa ccgattgaag ggattcattt tgttgactat
    57901 atggtcgagt ctattgtctc tctcacccat gaagcctttg ggcaacgggc gctggtggtc
    57961 gaaattatgg cggaagggat gcgtaaccca caggtcgccg ccatgcttaa aaataagcat
    58021 atgacgatca ctgaatttgt tgcccagcgg atgcgtgatg cccagcaaaa aggcgagata
    58081 agcccagaca tcaacacgtc aatgacttca cgtttactgc tggatctgac ctacggtgta
    58141 ctggccgata tcgaagcgga agacctggcg cgtgaagcgt cgtttgctca gggattacgc
    58201 gcgatgattg gcggtatctt aaccgcatcc tgatcctctc atttcgcggg ctggtgataa
    58261 ctgtgcccgc gtttcatact ttaatttccc tgtacaaaat acaatctagc cgggatcgga
    58321 gaattccccc caaaaatatt caccgtagcc atatgtcatg agagtttatc gttctcaaga
    58381 tgctcgaacg aacgttcgat tgcttatttt atggcttctg tcaacgctgt tttaaagatt
    58441 aatgcgatca atatcacgct gtgggtattg cagattttgg ttttttgatc gcggtgtcag
    58501 ttctttttat ttccatttct cttccatggg tttctcacag ataactgtgt gcaacacaga
    58561 attggttaac taatcagatt aaaggttgac cagtattttt atttgaatga ggagtccctt
    58621 atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
    58681 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
    58741 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
    58801 cgtaattatg tgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca
    58861 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
    58921 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
    58981 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
    59041 cagactatcc cgccgggaat ggtgattacc gatgaaaacg gcaagaaaaa gcagtcttac
    59101 ttccatgatt tctttaacta tgccgggatc catcgcagcg taatggtcta caccacgccg
    59161 aacacctggg tggacgatat caccgtggtg acgcatgttg cgcaagactg taaccacgcg
    59221 tctgttgact ggcaggtggt agcaaatggt gatgtcagcg ttgaactgcg tgatgcggat
    59281 cagcaggtgg ttgcaactgg acaaggtacc agcgggactt tgcaagtggt gaatccccac
    59341 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
    59401 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
    59461 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
    59521 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
    59581 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
    59641 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
    59701 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
    59761 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
    59821 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
    59881 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
    59941 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
    60001 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
    60061 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
    60121 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
    60181 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
    60241 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
    60301 ttgcgcgttg gaggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
    60361 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
    60421 ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt cggctacagc ctcggtgacg
    60481 tcgccaataa cttcgccttc gcaatggggg cgctcttcct gttgagttac tacaccgacg
    60541 tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt actggtgcgg gtattcgatg
    60601 ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt gaatacccgc tggggaaaat
    60661 tccgcccgtt tttactcttc ggtactgcgc cgttaatgat cttcagcgtg ctggtattct
    60721 gggtaccgac cgactggagc catagcagca aagtagtgta tgcatatttg acctacatgg
    60781 gcctcgggct ttgctacagc ctggtgaata ttccttatgg ttcatttgct accgcgatga
    60841 cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg cgggattgcc gcttcattaa
    60901 cctttgtctg cctggcattt ctgataggac cgagcattaa gaactccagc ccggaagaga
    60961 tggtgtcggt ataccatttc tggacgattg tgctggcgat tgccggaatg gtgctttact
    61021 tcatctgctt caaatcgacg cgtgagaatg tggtacgtat cgtggcgcaa ccgtcattga
    61081 agatcagtct gcaaaccctg aaacggaatc gcccgctgtt tatgttgtgc atcggtgcgc
    61141 tgtgtgtgct gatttcgacc ttcgcggtca gcgcctcgtc gttgttctac gtgcgctatg
    61201 tgttaaatga taccgggctg ttcactgtgc tggtactggt gcaaaacctg gttggtactg
    61261 tggcatcggc accgctggtg ccggggatgg tcgcgaggat cggtaaaaag aataccttcc
    61321 tgattggcgc tttgctggga acctgcggtt atctgctatt cttctgggtt tccgtctggt
    61381 cactgccggt ggcgttggtt gcgttggcca tcgcttcaat tggtcagggc gttactatga
    61441 ctgtgatgtg ggcactggaa gctgataccg tagaatacgg tgaatacctg accggcgtgc
    61501 gaattgaagg gctcacctat tcactattct catttacccg taaatgcggt caggcaatcg
    61561 gaggttcaat tcctgccttt attttgggat taagcggata tatcgccaat caggtgcaaa
    61621 cgccggaagt tattatgggc atccgcacat caattgcctt agtaccttgc ggatttatgc
    61681 tactggcatt cgttattatc tggttttatc cgctcacgga taaaaaattc aaagaaatcg
    61741 tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt aatcagcgat atcactaatt
    61801 aatattcaat aaaaataatc agaacatcaa aggtgcaact atgagaaaaa tagtggccat
    61861 ggccgttatt tgcctgacgg ctgcctctgg ccttacctct gcttatgcgg cgcaactggc
    61921 tgacgatgaa gcgggactac gcatcagact gaaaaacgaa ttgcgcaggg cggataagcc
    61981 cagtgctggc gcgggaagag atatttacgc atgggtacag ggaggattgc tcgatttcaa
    62041 tagtggttat tattccaata ttattggcgt tgaaggcggg gcgtattatg tttataaatt
    62101 aggtgctcgt gctgatatga gtacccggtg gtatcttgat ggtgataaaa gttttggctt
    62161 tgccctgggg gcagtaaaaa taaaacccag tgaaaatagc ctgcttaaat taggtcgctt
    62221 cgggacggat tatagttatg gtagcttacc ttatcgtatt ccgttaatgg ctggcagttc
    62281 gcaacgtaca ttaccgacag tttctgaagg agcattaggt tattgggctt taacaccaaa
    62341 tattgatctg tggggaatgt ggcgttcacg agtattttta tggactgatt caacaaccgg
    62401 tattcgtgat gaaggggtgt ataacagcca gacgggaaaa tacgataaac atcgcgcacg
    62461 ttctttttta gccgccagtt ggcatgatga taccagtcgc tattctctgg gggcatcggt
    62521 acagaaagat gtttccaatc agatacaaag tattctcgag aaaagcatac cgctcgaccc
    62581 gaattatacg ttgaaagggg agttgctcgg cttttacgcg cagctcgaag gtttaagtcg
    62641 taataccagc cagcccaatg aaacggcgtt ggttagtgga caattgacct ggaatgcgcc
    62701 gtggggaagc gtatttggca gtggtggtta tttgcgccat gcaatgaatg gtgccgtggt
    62761 ggataccgac attggctatc ccttttcatt aagtcttgat cgtaaccgtg aaggaatgca
    62821 gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa tttacactga tatttgcacc
    62881 gattgtgact cgcggctatg aatccagtaa acgagatgtg cggattgaag gcgcgggtat
    62941 cttaggtggt atgaactatc gggtcagcga agggccgtta caagggatga atttctttct
    63001 tgctgccgat aaagggcggg aaaagcgcga tggcagtacg ctgggcgatc gcctgaatta
    63061 ctgggatgtg aaaatgagta ttcagtatga ctttatgctg aagtaaaaaa taacgccgga
    63121 gagaaaaatc tccggcgttt tggctgtgaa ttactgctgc ggaatagcgg gtacgaccgg
    63181 aacatttaat gccagcattg caaacatacc aacgaaatct tccagtggca ttttctgccc
    63241 gtttaacgtt atctgaccgt tagtatattg caggctggtg gtgatggtat tgtcctgcaa
    63301 ggtggtcaga cggaacatct gccccattgc tgatgcacct tcaacttgct gtttcgccag
    63361 ttttttcgct tgatcttcct gataaccttc cagcttcgct acctgagtca taaactcagt
    63421 tgccatatcc accggaatgg tcagtttcgc atccagagat ttaaccgaac gatctacttc
    63481 ctgcgccagc gtttgcggcg cttctttagt cgttgccgga tctttcagga acagcgacag
    63541 attcagcgca ctttcaccct gactgttttt ccagcttagc ggcgcgatag taatcaccgg
    63601 atcgcctttc aacatcagcg gcagggcgct aaagaaggct tccgtcactt tctcctgata
    63661 aagttcggga ttgttggcaa tttctggctg tgccagcagc gcctgagttt gcgcgttata
    63721 ttgctggcta aactgatgcc aggcttcacc atcaatctgg ccgactttta aagtcagctt
    63781 gccgctgccc agatcctgat tctgtacctt caggctgttt agcgagtaat ccagttggct
    63841 attgatcgtt ttaccgtcat tgaccagatc cgatttaccg ttgatctcca tgctttccag
    63901 cagtgccagt tctttgcctt ccactgaaat ggtcattttt tccagtgaca gtttttggtt
    63961 tcctacgcgc tcaccaaaac ttgccagcgt gctggaaccg tcggttttca gattattaaa
    64021 ggtcaactgc actttctggt tgtattcgtt aactgcgtct atccgaccac tttgcgcctc
    64081 cccggaaagg gagatggctt tgccgtctct gtcagcattt aactggaact cgccgccgct
    64141 aaaggcgact ttttcatcct tttgctcgta attcagtggc ttgagcgaaa tatcggaact
    64201 ggaatcaccg ctgtaaccaa tgcgcgagtt aatctcaaaa ggcgtttcac cttttgccat
    64261 atcaaacagt ggtttgctta cttcgttatt aaccagcgtg gtttgaattg atgccatcga
    64321 cgggatcagg ttcagttttt taagctgggc aagcgggaag ggaccatgat caaccgattc
    64381 gttgaagatg acgctctgac cgcttttaat ccacggattt tctttcccgg caatgggttt
    64441 caccaacagt tgcaactggc tgctgaatac gccgcgatga tagttttgat aactcacttc
    64501 caggttggat tcaggagctg tcagtttgag ttgcgcgttc gcctgcgcga ccatgtcttc
    64561 gagatgggtt tcaatcttct tgcctgtata ccatgcgccg cctgtccaga ctacgcctag
    64621 cgcaacaatg acgcctaccg ctaccagcga tttattcata atgattatcc ataaaatgaa
    64681 atcaggcgga ctggccgcct gaaggtgtta taagccttta ataagcttag caagagatgt
    64741 taattttttc agtaagctct tacagcttgt tgtaaacacg cgctaaacgg ccgtggcctt
    64801 tgacagtcac cggtgattcg ttggcggcaa taaacgctga ttcacccggt ttaagctgta
    64861 actgctgaga acctttccac aacgttgcat cgccttcgac gcagaacaaa atggcggcac
    64921 tctgctggct aatggtggtt tctttatcac taaggtcatg cagcgagaag gcaaaatcat
    64981 ccactggaat cgggaagtcc agttctgcac cttgtttcac cggctgggtc aacaactggt
    65041 tagccggttt ggcttcgaat ttcacattgg caaccagttc cggaatatca atgtacttag
    65101 gcgtcagacc cgcacgcagc acgttatcgg agttcgccat cacttccagc gccacgcctt
    65161 gcaggtaagc gtgcggtgtt tcagcgaaca ggaacatcgc ttcgccagga ttcaatttca
    65221 ccacattcag taacagcggg gagaacaggc cgctgtcttc cgggtaaaat tcagaaatta
    65281 aacgaatcgt ttgccacggt tcaccctgct gactgtctag cgccgatttt aaaatcgcca
    65341 gcgcgcggga tttttcttca ccctgcatat tcaacaggct ggcgaacagt tcgcttaaac
    65401 gttcggcatt aggctgttgt aaaaagtgag caattgccgg atgtgcacct gcgactggct
    65461 ggagtaggga gacaatctcg gaaaactcac gaaacgcgtt catcgcaagg aaaggcgtca
    65521 gcgcaaaaac cagctccggc ttgtggttag gatctttata gttacgctcg gcggcatcca
    65581 tcgggatacc tgcggcattt tctttggcaa aaccgatttc agaattgcgt ttgtttggat
    65641 gaacctgaat ggagagcggc tgtgctgcgc ataatacttt gaacaggaaa ggcagttcgc
    65701 caaagcgttt ggcaacggcc tctccgagca gagtcgattt attactctca atcacatcac
    65761 gcagtgaaac gatatctccg gcggcattct gcacccgtga actgcttttc ggatgtgcgc
    65821 ccatccacag ctcggccatc ggctggctgg acggattttc cataccataa agttcagtca
    65881 acgccgtttt gctgccccag gcatagtttt gcactgagtt aatgagtttt tgcatgatca
    65941 atccctgttt taatgtggaa attaatcccg ctattaaagc aagaatccta cgggaagtaa
    66001 cctggagccg caaaaagtcg tactagtctc agtttttgtt aaaaaagtgt gtaggatatt
    66061 gttactcgct tttaacaggg caacggaaca cccgcccaga gcataaccaa accaggcagt
    66121 aagtgagaga acaatgtcaa acaaaccctt tcattatcag gctccttttc cactcaaaaa
    66181 agatgatact gagtattacc tgctaaccag cgaacacgtt agcgtatctg aatttgaagg
    66241 gcaggagatt ttgaaagtcg cacccgaagc gttaactctg ttggcgcgtc aggcatttca
    66301 tgatgcgtca tttatgctgc gtccggctca ccaacaacag gtggccgaca ttctgcgtga
    66361 cccggaagcc agcgaaaatg ataaatatgt ggcgctgcaa ttcctgcgca actccgacat
    66421 cgcggcgaaa ggtgttctgc caacctgtca ggataccggc actgcgatta ttgttggtaa
    66481 aaaagggcag cgtgtatgga ccggtggctg tgatgaagcg gcgctggcgc gcggtgtcta
    66541 taacacttat atcgaagata atttgcgcta ctcgcaaaac gcgccgctgg atatgtataa
    66601 agaggtgaat accggcacca atctgccagc gcagatcgat ctttatgccg ttgatggcga
    66661 cgagtacaaa ttcctctgta tcgccaaagg tggtggttcg gcaaacaaga cgtatctcta
    66721 tcaggaaacc aaagcgttac taacgccggg gaaactgaaa aattacctgg ttgagaagat
    66781 gcgcacgctg ggtacggcgg cctgtcctcc gtatcatatt gcgttcgtaa ttggtggaac
    66841 ttctgcagaa actaacctta aaacagtgaa actggcttcc gctaaatact atgacgaact
    66901 gccaacggaa gggaatgagc acggtcaggc attccgcgat gtggaactgg aaaaagaatt
    66961 gctgatcgaa gcgcaaaatc tcggtctggg tgcgcagttt ggtggtaaat acttcgctca
    67021 cgacatacgc gtgattcgcc tgccacgtca cggcgcatcc tgtccggttg gtatgggtgt
    67081 ttcctgctct gctgaccgta atatcaaagc gaagatcaac cgtcagggga tctggatcga
    67141 aaaactggaa cataatccag gtaaatatat ccctgaagag ctgcgtaaag cgggagaagg
    67201 cgaagcggtg cgcgttgacc ttaaccgtcc gatgaaagag atcctcgcac agttgtcgca
    67261 gtatcccgtt tctacacgct tatcgcttaa cggcacgatt atcgtcggtc gtgatattgc
    67321 tcacgccaaa ctgaaagagc ggatggataa cggtgaaggg ctgccgcagt acattaaaga
    67381 tcatccgatt tactacgcgg gtccggccaa aacgccggaa ggttatgcct ccggttctct
    67441 tggcccaacg accgccggac ggatggattc ttatgtcgat caactgcaag cgcagggcgg
    67501 aagtatgatc atgctggcga aaggcaaccg cagccagcag gtgacggatg cctgtaaaaa
    67561 acacggcggc ttctaccttg gcagtatcgg tggtccggcc gctgtattgg cgcagggaag
    67621 tattaagagc ctggaatgtg ttgaatatcc ggaactggga atggaagcca tctggaaaat
    67681 tgaagtggaa gatttcccgg cgtttatcct tgtggatgat aaaggaaatg acttcttcca
    67741 gcagatacaa ctcacacagt gcactcgctg tgtgaaataa acagagccgc ccttcggggc
    67801 ggttttttta catggcacga aagaccaaac atttgttatc aaatagtaaa taataagtga
    67861 gctaaaagtt gcttaacgaa agcaaaacag aaagaaaaaa ttaaacaggt gaggagcagg
    67921 ccatgaatac agtacgcagc gaaaaagatt cgatgggggc gattgatgtc ccggcagata
    67981 agctgtgggg cgcacaaact caacgctcgc tggagcattt ccgcatttcg acggagaaaa
    68041 tgcccacctc actgattcat gcgctggcgc taaccaagcg cgcagcggca aaagttaatg
    68101 aagatttagg cttgttgtct gaagagaaag cgagcgccat tcggcaggcg gcggatgaag
    68161 tactggcagg acagcatgac gacgaattcc cgctggctat ctggcagacc ggctccggca
    68221 cgcaaagtaa catgaacatg aacgaagtgc tggctaaccg ggccagtgaa ttactcggcg
    68281 gcgtgcgcgg gatggaacgt aaagttcacc ctaacgacga cgtgaacaaa agccaaagtt
    68341 ccaacgatgt ctttccgacg gcgatgcacg ttgcggcgct gctggcgctg cgcaagcaac
    68401 tcattccgca gcttaaaacc ctgacacaga cactgagtga aaaatcgcgt gcatttgccg
    68461 atatcgtcaa aatcggtcga acccacttgc aggacgccac gccgctaaca ctagggcagg
    68521 agatttccgg ctgggtagcg atgctcgagc ataatctcaa acatatcgaa tacagcctgc
    68581 ctcacgtagc ggaactggct cttggcggta cagcggtggg tactggacta aatacccatc
    68641 cggagtatgc gcgtcgcgta gcagatgaac tggcagtcat tacctgtgca ccgtttgtta
    68701 ccgcgccgaa caaatttgaa gcgcttgcga cctgtgatgc cctggttcag gcgcacggcg
    68761 cgttgaaagg gttggctgcg tcactgatga aaatcgccaa tgatgtccgc tggctggcct
    68821 ctggcccgcg ctgcggaatt ggtgaaatct caatcccgga aaatgagccg ggcagctcaa
    68881 tcatgccggg gaaagtgaac ccaacccagt gtgaagcatt aaccatgctc tgctgtcagg
    68941 taatggggaa cgacgtggcg atcaacatgg gtggcgcttc cggtaacttt gaactgaacg
    69001 tcttccgtcc gatggtgatc cataatttcc tgcaatcggt gcgcttgctg gcagatggca
    69061 tggaaagttt taacaaacac tgcgcagtgg gtattgaacc gaatcgtgag cgaatcaatc
    69121 aattactcaa tgaatcgctg atgctggtga ctgcgcttaa cacccacatt ggttatgaca
    69181 aagccgcgga gatcgccaaa aaagcgcata aagaagggct gaccttaaaa gctgcggccc
    69241 ttgcgctggg gtatcttagc gaagccgagt ttgacagctg ggtacggcca gaacagatgg
    69301 tcggcagtat gaaagccggg cgttaatctg caacatacag gtgcagccgt ggaatgatca
    69361 aacgaagcgg ctgcgcctgt ggtttataac ggtgctgcac attgtcggca tcgtaattta
    69421 acaactcacc aacgtctggc acgcccgcac cattatcccg attaatcagt gcaatcagcg
    69481 gtgtagggca ggcgtgttgc acttgttttt gatctccttt gtaccagacg cgggcaatcg
    69541 gctgcacctt caccggacgt ttgattttta acttcgcgtt ctgtggcagg gcagcgatat
    69601 cctgatactc tcgctccagt tttctttgcc actcctcgcg cgtccacggt gcgacactgc
    69661 gtggtgattt caggcttttt tccagctgtg ccaggacttc atcacgatgc aaattcttaa
    69721 tgatatgttt attagcccaa ccaaagcgta aagtggcggg gtcgtgcaga acggtgagcg
    69781 tgcggtaagc attaagggtg atcagccccg gcaaatgacg atgcacccat tcaaaacgtg
    69841 ccgcggtggg gagttctgat tcaaccgtga cgatatgctc gaacgtggtc ttgagtttat
    69901 tgatgtgctg aatatgactg accaacgctg cttgcgaaag gttatcgacc tggtaacaca
    69961 acacgccagg cagacggaca gcggccttgc tgctgcgatt ttcggactgt tgttgaataa
    70021 ataaatggcg gaaatgacgc aacgccagcg actgcgcgtc gttgccgaga tgttgtttta
    70081 cctcaatacg attaagcgga ttatgctcat cctctttttt tacctccggc aaagagaaca
    70141 cgcgggcaac caatagcttg tgttgctcaa gatgagcggc aaatgcagcc agctcttgtt
    70201 ccatctggcg aaaggtagtg ttgagtcggt ctacgagatc gtaacgcgcc ataatattaa
    70261 ccactttagt tacaacatac ttattttata gcacagtggt ggtgacttga ccatcgtgcg
    70321 cgtagtgtca ggcagaggta aattgcggga tgttatgcca taacggccag ctaaacgaga
    70381 agcgagcacc acccagttcg ctggtgtcac aattaaccgt accgcccatt gccagtgcta
    70441 tagagtggac aattgccagc cccagcccgc agccgccggt tgagcgatcc cggctgggat
    70501 cgaggcgaac aaaaggttca aagatatgtt cgcggttttc tggggcaatc cctgggccat
    70561 catcctcaac aattaatgtc gctctattcc ccgacagtag caggctggtt tcaaccgttg
    70621 aatggcagta ccgcagggcg ttattgagca aattatccag cacgcgctcc attaagcgca
    70681 tatccaacgc cgcataatgg ccttgcgcga gcgttttaat ccgtaccgtt ttatcgggcg
    70741 ttactgcctg aatgtctgcc agatgcgttg acagccacaa cggcaggtct ggttcgctaa
    70801 gatgaagctc gttttgtggg cgatcgagtc gggcataagt cagcagctct tcaattaaag
    70861 cttcaagttg actgatatca cgattcaacg cctgggattc ggcggcgctc aggttatcgc
    70921 tcatctccag tcgataacgc aggcgcacta acggtgttcg cagttcgtga gcgataccgt
    70981 caataagctg ttttttgctg gcaatcaagg cgttgatatt gtccgccatc tggttaaatg
    71041 cgataccaag tcgttcaaag ctcgaaccct catcaaagtg gatacgttca ttgagatgcc
    71101 catcgccaaa tcgttgcgcc gctgcttcca gttttaacat atcctgccag tgcggacgca
    71161 tccagataaa caccggaaag gcgagggaaa tagcaataaa agtgatcagg gcgatatcca
    71221 gcaatcgcat ctgatggaga taataaagat aaggaacagg accaactgcc agcacgtagt
    71281 ggctgcgcgg gatacgctgc aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc
    71341 cacgcagtcg gtgcatggaa atatcatcaa gatggtattt actcagtggc tcgacacgca
    71401 gatcgaaaga gagatttaaa tccatctctt tcagcgtctt accccagtcg tgtgggggga
    71461 tcgatctctc gcaattcgct gcgcatcaga tacagcgaac tgttcatcaa atcatccagc
    71521 gactgtttgc ccgcgcgttc ggcggtaaat ttgtacacca gcccaaccag cagagacatc
    71581 acaaggaagc agacaaacaa taacaggtaa aactggataa acagtttttt catcgcttat
    71641 tcccatgcat gaggcgcaaa aagatagcct ttgttacgca cagttttaat gcgataaggt
    71701 tctgcggcgt tatcgagcag tttttttctt aaccgcgaaa tagccacgtc cacgctacga
    71761 tccagtccgt cataactgac gccgcgtaaa tttttcagca atgcatcgcg gtccatgatt
    71821 tgcccggcat gggtagctaa ttcccacaat aattcgaaat cagctgtcga gagcgagatt
    71881 tcagtgttag ccagggtgac tacgcggttg atgggatcga tggtcaacgt gccgaaatgc
    71941 agggctttgt agggagtcag agacgtttcc tgaagacctt tggtcagtgt ggcttgctca
    72001 ttctgacgca aatgcaaacg taaacgcgct agcaaaacag cagggggcgt cgttttgaga
    72061 atatagtcgc aggcacccat ttccagtgcc aggatgtggt tcatatcgct atcgagagag
    72121 gttagaagaa caatcggtcc agaccacttt gcgcgtaaat cacgacaaat ggtcatgccg
    72181 tccttgcctg gtagcatgat gtcgagtaac accaaatccg gactttctcg caaaatggtt
    72241 tcttcggcct ggtcgccgcg cggttctacg gtaacctgca tatcatgttt tgccaggtac
    72301 gcggcaatca gtgaaccgac ttccgcatca tcttccacaa atacgatagt gttcataaca
    72361 ttcacggtag atataaaaac gtcacaatac accgcgctgt ttttacttac tatcaatcgt
    72421 ttctaaacaa gttttcatca ggtattctac tgatgacttg ttatggaagt gttaaggtaa
    72481 aaagatgggg ctggtaatca aagccgcgct cggtgcgctg gtagtgctgt tgattggtgt
    72541 tttagcaaaa acgaaaaatt attatatcgc cgggctgatt ccacttttcc cgacctttgc
    72601 gcttatcgcg cattatattg ttgccagcga acgcggcatt gaagccttac gcgcaaccat
    72661 catttttagt atgtggtcga ttattcccta ttttgtttac ctggtgtcgc tgtggtattt
    72721 caccggaatg atgcgcttgc ccgccgcttt tgttggttct gttgcgtgtt gggggataag
    72781 cgcatgggtg ttgattatat gctggataaa gctgcattaa cgcagatgac gaccgccatc
    72841 aagtgggaaa ctgcgtccgg tgacaaagca gctggtaagt aagtaatcga ccaggtcgat
    72901 cacttctttc tcgccaggcg cggttttcat cagtgattta ttcagcgcct gttgtcgata
    72961 ttcggcatca tcatgttcat taaacaggat cagcgatggc gcaatagaat tcactttcac
    73021 ttccggtgcc agcttgcggg caaacgagcg ggtcatatta tccagtgccg ctttgcttgc
    73081 agcatacgca acatgtttgt cgctaccgcg ctccaccaca taatcggtaa agtgaatgat
    73141 atcgctggcg gcgtgtccgt gcccacgcag taatctttcc agcgcatggt tgagcaggta
    73201 tggggtatta acgtggatct gcatcatgca agccagtacg tcggccagtg gtgtacctgg
    73261 tttttccgcc atccacgcac tggcgttatg caaaatagca cgcagaccat gggtgctttt
    73321 tagtacttca tcggcaaacg ccatcacacc gtcgttggtc gaaaaatcag cctgaataca
    73381 ctgcgcacct gccttaatca gtccatcaat ggctggatag tgcgtccgat agctgacaat
    73441 caccggttgc ttttgattaa tgaaatgcca ggcgagggcg aggccgatgc gacgacctcc
    73501 gccagtaatt aatattggca agggctgggc tttacccatc gttatctcct ttgctatcca
    73561 acgatgggat gaacgttatc ccaccagcat ccaggtcgcc ggaactgagg caattaacag
    73621 catgccaatc agcaccattt cctgacggtt cagtacatta tcatgtgtat gcgttttgcg
    73681 cgcatacagg aaaaccagca gccccggcgc atacagaaca acggataaca gcaagtgcat
    73741 tggaccagag gcgtacaata accataagcc ataaatgcag gcaccgacac ctaccgcttt
    73801 atgcagtgga cgggttgcga ttttcagcaa gaacgcgccg accaggaaat agggcaccag
    73861 aatcatttct gaggcgatgg tcagcaacgt gttgtaatcg gaaccggtca gccagatgag
    73921 taccagacaa atttgcacgc agatgttggt caaccacagc gaggcggatg gcgcagcttg
    73981 tgcgttctgg cgcgcaaaaa tgcgtgggaa tgctttatga gtggcagcca ggaacggaac
    74041 ttctgccgcc atgatggtcc agcttaagta cgcgccgcaa acggaaacga tcaaaccggc
    74101 agcgatgatg atttcgcccc atggtcccat catttccacc atcagaccgg ccattgacgg
    74161 gttacgaatt tcagccagtt caggacgtgc caccacgccc agcgaaagca gcgttaccag
    74221 caagtaaacg cccagagcgg agagaaccgc cagcagtgtc gctttgccaa catcacgttt
    74281 attacgcgca cgcgcagaaa caaccacggc accttccaca ccaatgaaaa cccacagggt
    74341 gatcagcatg gtgtttttca cctgttccca cacgggtacg ccaagtgcaa gtccggtgaa
    74401 gtcgagcttg aaggtatcca gtttgaacat catcatcgcc agcacaacaa acagacccag
    74461 cggcaacaat tttgccagtg tcgccaccag gttaatgctg gcagcggttt gcaccccgcg
    74521 caggatcaaa aagtgaacaa tccacaataa agccgatgca ccgactatcg attgccaggt
    74581 attaccatcg ccgaacaggc gcaattccgg cgtgtccgta aaaaagctta acgcggaaaa
    74641 aacgatcacc agataggaga cgttggcgat gactgcgcac agccagtatc cccatgcgga
    74701 acaaaagccg attagctcgc caaacccttc gcgggcatag gtaaagatac cgccgtcaag
    74761 ttcgggacga atgcgcgtga ggatcagcat ggcaaaggcc agcaataaaa tgccagcgcc
    74821 agtaataccc cagccgatga gcagtgctgc cgggctggca actgccgcca tattttgcgg
    74881 cagactgaaa acacccgcgc ccagcattga gcttaatacc agcgcggtga gtgcgctcag
    74941 tcccagtttc ttttccatcg atatccagtg attaaaacgt gataagaaga ataaatatta
    75001 gggcaaaacg cataaaaatg gagtttgcca ctgaagcgcg ggattttacg aagagatgtg
    75061 tctgcatgca atggtcgcgg ggaaatttgt gactaaaaat gcgaaaggcg gcatcaagcc
    75121 gccttatagg agtaactgaa tagttattta tacagatctg cgctgacggt caggttatta
    75181 ccacgttcct gccactggcg ggtgatgtgg taatacttag cacctttctt cgcggcacgt
    75241 ttcgcaacct gataggagac ttcggtcatg ttgccgtagt tgccagagaa tttgatgctg
    75301 tcgaacggga ccatcatcgc tgcggtcgct ttgttcagtt cttcgacttt agtgccatcc
    75361 gggagcgtga cggtgtaacg cccgcctttt gatgactggg tttcaaagaa acgaccgact
    75421 tcagaacttg gtgatgcggt agtcgcaaca cctgggatct caactttctt cgcggcttcg
    75481 ccaccggcag ccagagctgc acgtcctgct tcggaatctg ccgggatcac atccgggctc
    75541 tggacgatac gtttcttagc atctttttta tagatgaatg cagtaatacg ctggttgccg
    75601 ccctggttgg catcgatttg acgaacgatg tagaaagagt aggcaccttt cgcttttgcc
    75661 gctttggtga tggcatcatt gacttctggc tggctacgat agaagccctg gacggtgacc
    75721 gtgtcaaacg gttcaatcag aacagcctga tctttcggca gttcgacaac accgttaatt
    75781 acgcgattac ttgtttcttc tgctttttca gcatcggctt tatagaggtc agcgaccaca
    75841 cgccagttac cgctgttacc aaaatcagaa gtgtcgacaa cataaaaaga ggcggcacct
    75901 tctttatctg cgcgacgaga aacggctttc accgcttcgc caatagcatt aaaacgaccg
    75961 gtaaccacta cacggtcaaa aggtttaacc gctgccgctt gctccggtgt cagttctgtt
    76021 gctgcgttaa cggagaatgc cgtagcagaa agcagtgccg acgccaggag ggtgttctta
    76081 agcttcataa aaataatcct tcgccttgcg caaaccaggt actggtattg ttattaacga
    76141 gaaacgtggc tgattattgc atttaaacgg tgtaactgtc tgcgtcattt ttcatatcac
    76201 attctttaag ccaattttaa tcctgctcaa atgaccgtct atgcttaaaa aacagccgta
    76261 tcagcatcat tactactgaa gcaactgaat tatataagtt aatttaatgt taagtagtga
    76321 ttcgtgccgg ggcgatgtct cgttttaccc gaccgtcgaa gacaattatc agtctttatc
    76381 cggcgttcta aggtgtttat cccactatca cggctgaatc gttaatattt tgcgagttca
    76441 cgccgaaata ctgatttttg gcgctagatc acaggcataa ttttcagtac gttatagggc
    76501 gtttgttact aatttatttt aacggagtaa catttagctc gtacatgagc ggcttgtgtg
    76561 gctcctgaca caggcaaacc atcatcaata aaaccgatgg aagggaatat catgcgaatt
    76621 ggcataccaa gagaacggtt aaccaatgaa acccgtgttg cagcaacgcc aaaaacagtg
    76681 gaacagctgc tgaaactggg ttttaccgtc gcggtagaga gcggcgcggg tcaactggca
    76741 agttttgacg ataaagcgtt tgtgcaagcg ggcgctgaaa ttgtagaagg gaatagcgtc
    76801 tggcagtcag agatcattct gaaggtcaat gcgccgttag atgatgaaat tgcgttactg
    76861 aatcctggga caacgctggt gagttttatc tggcctgcgc agaatccgga attaatgcaa
    76921 aaacttgcgg aacgtaacgt gaccgtgatg gcgatggatt ctgtgccgcg tatctcacgc
    76981 gcacaatcgc tggacgcact aagctcgatg gcgaacatcg ccggttatcg cgccattgtt
    77041 gaagcggcac atgaatttgg gcgcttcttt accgggcaaa ttactgcggc cgggaaagtg
    77101 ccaccggcaa aagtgatggt gattggtgcg ggtgttgcag gtctggccgc cattggcgca
    77161 gcaaacagtc tcggcgcgat tgtgcgtgca ttcgacaccc gcccggaagt gaaagaacaa
    77221 gttcaaagta tgggcgcgga attcctcgag ctggatttta aagaggaagc gggcagcggc
    77281 gatggctatg ccaaagtgat gtcggacgcg ttcatcaaag cggaaatgga actctttgcc
    77341 gcccaggcaa aagaggtcga tatcattgtc accaccgcgc ttattccagg caaaacagcg
    77401 ccgaagctaa ttacccgtga aatggttgac tccatgaagg cgggcagtgt gattgtcgac
    77461 ctggcagccc aaaacggtgg caactgtgaa tacaccgtgc cgggtgaaat cttcactacg
    77521 gaaaatggtg tcaaagtgat tggttatacc gatcttccgg gccgtctgcc gacgcaatcc
    77581 tcacagcttt acggtactaa cctcgttaat ctgctgaaac tgttgtgcaa agagaaagac
    77641 ggcaatatca ctgttgattt tgatgatgtg gtgattcgcg gcgtgaccgt gatccgtgcg
    77701 ggcgaaatta cctggccggc accgccgatt caggtatcag ctcagccgca ggcggcacaa
    77761 aaagcggcac cggaagtgaa aactgaggaa aaatgtgcct gctcaccgtg gcgtaaatac
    77821 gcattgatgg cgctggcaat catccttttc ggttggctgg caagcgttgc gccgaaagag
    77881 tttcttgggc acttcaccgt tttcgcgctg gcctgcgttg tcggttatta cgtggtgtgg
    77941 aatgtatcgc atgcgctgca tacaccgttg atgtcggtca ccaacgcgat ttcagggatt
    78001 attgttgtcg gagcactgtt gcagattggc cagggcggct gggttagctt ccttagtttt
    78061 atcgcggtgc ttatagccag cattaatatt ttcggtggct tcaccgtgac tcagcgcatg
    78121 ctgaaaatgt tccgcaaaaa ttaaggggta acatatgtct ggaggattag ttacagctgc
    78181 atacattgtt gccgcgatcc tgtttatctt cagtctggcc ggtctttcga aacatgaaac
    78241 gtctcgccag ggtaacaact tcggtatcgc cgggatggcg attgcgttaa tcgcaaccat
    78301 ttttggaccg gatacgggta atgttggctg gatcttgctg gcgatggtca ttggtggggc
    78361 aattggtatc cgtctggcga agaaagttga aatgaccgaa atgccagaac tggtggcgat
    78421 cctgcatagc ttcgtgggtc tggcggcagt gctggttggc tttaacagtt atctgcatca
    78481 tgacgcggga atggcaccga ttctggtcaa tattcacctg acggaagtgt tcctcggtat
    78541 cttcatcggg gcggtaacgt tcacgggttc ggtggtggcg ttcggcaaac tgtgtggcaa
    78601 gatttcgtct aaaccgttga tgctgccaaa ccgtcacaaa atgaacctgg cggctctggt
    78661 cgtttccttc ctgctgctga ttgtatttgt tcgcacggac agcgtcggcc tgcaagtgct
    78721 ggcattgctg ataatgaccg ccattgcgct ggtattcggc tggcatttag tcgcctccat
    78781 cggtggtgca gatatgccag tggtggtgtc gatgctgaac tcgtactccg gctgggcggc
    78841 tgcggctgcg ggctttatgc tcagcaacga cctgctgatt gtgaccggtg cgctggtcgg
    78901 ttcttcgggg gctatccttt cttacattat gtgtaaggcg atgaaccgtt cctttatcag
    78961 cgttattgcg ggtggtttcg gcaccgacgg ctcttctact ggcgatgatc aggaagtggg
    79021 tgagcaccgc gaaatcaccg cagaagagac agcggaactg ctgaaaaact cccattcagt
    79081 gatcattact ccggggtacg gcatggcagt cgcgcaggcg caatatcctg tcgctgaaat
    79141 taccgagaaa ctccgcgctc gtggtatcaa cgtgcgtttc ggtatccacc cggttgcggg
    79201 gcgtttgcct ggacatatga acgtattgct ggctgaagca aaagtaccgt atgacatcgt
    79261 gctggaaatg gacgagatca acgatgactt tgctgatacc gataccgtac tggtgattgg
    79321 tgctaacgat acggttaacc cggcggcgca ggatgatccg aagagtccga ttgctggtat
    79381 gcctgtgctg gaagtgtgga aagcgcagaa cgtgattgtc tttaaacgtt cgatgaacac
    79441 tggctatgct ggtgtgcaaa acccgctgtt cttcaaggaa aacacccaca tgctgtttgg
    79501 tgacgccaaa gccagcgtgg atgcaatcct gaaagctctg taaccctgac ggcctctgcg
    79561 gaggccgtca ctctttattg agatcgctta acagaacggc gatgctttga cctcccgctg
    79621 tttgttcaag cgcaattttg acaataattg ttaacggcac ggaaagcagc atacccaccg
    79681 gtcctaacaa ccatccccaa aaaatcaacg acaaaaatac caccaatgtg gaaagcccca
    79741 gcccacgtcc catgatgcgc ggctccagaa tattgccgaa gaccagatta atcagcagat
    79801 atcccgccag caccagcaac gcttcgtaga agccattaaa caccagtacc tgagcgatag
    79861 gggggattgc cgcgagaact gaaccaatat tagggatgta attaaacgca aaggccatca
    79921 atccccagac aaaagcgaag cgaacatcga gtgcggcgag catcgcccag gcgaccaggc
    79981 cggtgatgat gctgatggct gttttcagca ccagataatg agaaacactg tcaattgcac
    80041 gttgaatcgc cgccatccct tcaaccggac gcgccatcat ttgctgaaat tttccgggca
    80101 attgtggcac ttcgagcagc ataaacagca ccgtcagcag caataaaaat attgatgaca
    80161 tggcattaga taactgcgtc aataagttgg tgagcaacgt catcgccgcg ttcggatcaa
    80221 tgtaatgcgc cagctggtca actgagacgt caatccctac gcgttgcaac aacggctcaa
    80281 gagcttgcag cggcgtcata atagagttgc gatattgcgg taacgtccgc gtcaactcgt
    80341 tgagcgcgga acccagataa gctaatagca acaccatcgc catcacgatg atggtcatca
    80401 aaatcgacac cgccagtaca cgcggcactc gccagcggac catgtgttgc accagcgggt
    80461 taagaataac agcattttat catttagtgt tttttgctgt ttcataatgt ttcaggctgt
    80521 gcattatgtt tttgattatc aacagatttg tgattttcct tgttccagct tgtttcacgt
    80581 tgtagccctt tgtgcaacga tcggtgtagt cactggtgta gtcacttttt cgtaaagagg
    80641 gggtaaaacg tgagcagagc aatgaacaaa ctgagcgata ccttgctaag gaaacttcat
    80701 ggtaagccag cggagaaaaa cacgttttat agtgatggcg gaaacctgag cgtgaaatat
    80761 ttaacatcag ggaaattgac ctggtatttc acatacaggg ccggaacggg aagggagaca
    80821 cgacccgaac gcattaagct gggaagttat cctgatctga gcctgaaagc agccagggaa
    80881 aaagccgcac agtgtcgcac atggctggct gaggggaaaa atccacgtca tgagatgaat
    80941 tacaccgtac aggaagcgtt aaagcccgta acggttggcg atgcgctcac ctactggctt
    81001 gagtattacg taaaggaaaa ccgcgtggat tatatcgccc tgaaaaggcg acttaataat
    81061 cacgtaatac agcagattgg tgctatgccg ctggataaat gcgagctacg gcactggctg
    81121 gcctgttttg accaggtggc aaagcgaacg cctgttactg ccggattcgt gctacaggcg
    81181 tgcaaacagg cgcttaagtt ctgccgtagg cggcgctatg caatcagcaa cgttctggac
    81241 gatctgaacg ttgcggacgt tgggaaaaaa ccggatataa gcgaacgtgt cttaagtaac
    81301 aaagaacttg gcgaattatt gcaggcactg gacaaaaaaa tattctcccc ttactacgtc
    81361 gcgttaatcc gcctcctgat tgtcttcgga gccagaacgg tagaactgag gttatcggag
    81421 atcggggagt gggattttac ggaaatgcta tggaccgttc cgaaggagca cagcaaaacg
    81481 aaggtcgcaa tattccggcc cataccggaa gcgatcctgc cgttcgtcac gcagctggtg
    81541 gagcagaaca ggcacacggg cttattgctg ggggaagtga aacaggaggc cagcgtatcg
    81601 cagtatggaa gattagcgca caggaggctt aatcaccctc actggtcact gcatgacatc
    81661 cggcgcacct ttacaaccat gctgaacgat ttaggcgttg atccgcatgt cgtggagcag
    81721 cttacaggcc accagatgcc aggaatgcag cgagtttata atcattcccg ttatctggat
    81781 gcgaaacgca atgcgctgga tatgtggacg gagcggttag ggatactggc gggaacacat
    81841 gaaaacgtaa ccacgctacc cgtagccaga agaaaataat ttttttcgtg ttttttcagt
    81901 atgcgcatac tggatatgcg aacagataca acgtgcaaca atgagcaaca atgtggaaca
    81961 actacgaaca agaggcgaaa aagcgtacgg atttataagt agctgatttt taatgcgtta
    82021 ctggtttttt gtacactagc gaatcactct ttaaagagcg agaaaaaagg catggaacaa
    82081 tattttatgc ctttgtatta acatgaattt aaaagatttt attcttactg tttcattttc
    82141 tgtaaatcat acatcctcag tatttgccat tcatcactct agcgggaaca atacgacaca
    82201 ataagacacc tgatgactct ttaagaaacg aaaggggggc aatagtgtta agcactgatc
    82261 ggtttatacg tgaaaaagaa tgcgaaaaac taaccggcct tagccgtacg tgtcgctacc
    82321 gcctggaaaa ggccggacaa ttcccatcac gtcgtaaact tggcggtcgt tccgttggct
    82381 ggtctttatc cgaggttctg gcctggaagg atagctgcaa ggcagttcat taatcacgct
    82441 ggcggcacgc agccgccaca catcaatcat ctgaacacag agctataacc atgaagattg
    82501 aatatacgcc agaacgtggg cggggattcg ttcgccctgg tgaaactgaa aaacaacaaa
    82561 attggggttt ttcaggtata aaaaaagcgg ccccgaaatg gagccgcctt tctgaacaga
    82621 taacccgctg cgccgtttgt gtgtgtgatc ccaaacataa gcacggggat gatagccgtt
    82681 atcaggctgg tgggcaatgc aatcagtctg gttcagttcg ttgccatacc tgcaatgagc
    82741 gcttttccct gtactcttta aggaattgct caagggcaaa agcacatggc gcgaatcttt
    82801 ctgattcatg ctctatcttt ctgcgccgtc ttttccgtgc cggtgataat gttttggtca
    82861 attctttatc ggtcattgtg ttgtcctgca tagcaatgcg ccgtaatacc ttacaccacg
    82921 gcgctgatgg tgattactct ggttctttgg ccttgcgccg ctggcgatat tcaacttctc
    82981 gttttaatgc cgcagtaaca aattgccccg tactttcgcc aggcattttt accgcctcaa
    83041 cattgttcat aacttcatgc ggaacccttg ccgcaacggt ttgtgatttt gcgtttaccg
    83101 cttttgtcgc catgtggtgt acccctcata aaaaacaaat gcagtatgca ggaaaaaaaa
    83161 taagtgttca acacttgacg tgtttaacac ctgggtttaa attggtgttc aacaccttgt
    83221 tggcgcaagg tgcagaaacg acgaaacccg gcagtgcgct aacactaacc gggcttctaa
    83281 ccaccaacga tagcgaaagt atcgaggtag ctatgagaaa tcataccaca catccgcaag
    83341 ggcgggactc gcacaacctg aataaataca tctggcgttt tatcgccctg agcacggcac
    83401 aaccgcgcgt gattaccatt gaggccacca gcgaacagga agcacgccag caatctccgg
    83461 ctggctgcgt gatggtattt gctgcccgta ttcgtcaggg ggtaggctta tgagccagga
    83521 aatcacacta caacaggcag cagaacgcgc ccaccaaatc gaagttattt gcgcactggc
    83581 agaggattac cctggcatga tgaccgacag cgaatcaggg gcaatcatcg gcttacttaa
    83641 acgccttagc ggtgaggtct gcgtattcct gagcgatgaa caggaaagaa gaacgcttat
    83701 ttctaacgaa aaaaaatgcg gaggggtaca tgtgcaataa aaccacaccg gacgcagccg
    83761 ccgccgcgct cactacgctg atgcacgcgc ttattgatat ttctgttatt gctgacaggg
    83821 cgcataaaca cgcagccaga gaatcagaat gcatcttcca ttatttagca tttgtgcagc
    83881 taaaagccga tcaggcactg gataaggccg gaaaaattat catggctgat gtgcaggagg
    83941 tgcaccatgc ataatctgtc aatttctgac cttaacagca ttcagtttga tgagaaattt
    84001 accgggcagt tgctggtcaa tgtggagaac gggcgcatag tgcgtaatta tcacctgccg
    84061 gatggtgcaa ttgccggaag cgttgaagca ttgctggaac tggcggaacg tgcgcgactg
    84121 attaagccgt caacgagcca tcacgatgat gatctgcatt ttaccggatg tatggtgagt
    84181 cactacgaaa acggcgtcga ggtatcctgt gaacggctgc gtgatgattg ctgtttcggc
    84241 acactgccgg aatttatcga gttgctgacc agttgcggtt atcaggtcat tcaggggggt
    84301 aaacatgcgt gatgatcgtt ttaattccct gaaacaggaa ttttccggcg ttcctgatga
    84361 tgcggctgat gcgctttcgt caatatccaa actgattaga gcatcttttt tcttaattgg
    84421 cacgaaagaa tataaatcaa ctggaataga cgtactgaat atcgccgccg attatgctga
    84481 tttcgtgaca gaggttattt taagaaaaac gacggacggg gattaatatg cgtgatattt
    84541 accacgaaac aatataccgc gcatttcttg cactttctca cagtgaaaat atgttggaaa
    84601 tattgcgcat atggcatgaa acacttggcg acaatgaatg cgacaaacaa aaatcaagaa
    84661 ttgttacggc attaataacg cttcttgagc ctgtaataat ggaactgcaa gaaatagacc
    84721 tattgcacga cagatataaa gaacagcaca ccggagaata aaaataatga aacttaaata
    84781 tcctggctta actgccagcg gaaaaactcg cactaaattc atgtgcgggg atatttaccg
    84841 cgataagtac ggcggcacgg taatgattaa gggcgtggag gaacggcgcg taacttaccg
    84901 ccgtgaaggc tacgaatatg attgcgttat gcctgtttat cagttcgacc gtgatttttc
    84961 tctggtgcag gctgtaccgc gtaacgtgcc caccagcagg gagaaagcac gcgccaatat
    85021 tcaggaaata agaaagatgt tgaacgtatt caggggtaaa aaatgaaact tgcaccgaac
    85081 gtaaaacagc agtcacgcgg cataaaacac aaagaaacag aagtcattat ttttgcgggt
    85141 agtgatgcct ggtcacacgc aaaacaatgg caggaacatg acgcgcgtat ggccggagat
    85201 aatgagcctc ctgtgtggct tggggagcag cagttatccg aactggataa gctgcaaatt
    85261 gtgccggaag gcagaaaatc cgtgcgcata ttcagggccg gatatcttgc gccagtaatg
    85321 ataaaggcga ttggtcagaa gctggcggcg gcaggcgtac aggatgcaaa tttttaccct
    85381 gatggtatgc acggtcagaa ggtggagaac tggcgcgaat atctggcccg tgagcgccag
    85441 aatctttctg aaggtctgtt cgttgagctt ccggtaaagc aaaaggcgca actttcgcag
    85501 atggcggaca gtgaacgcgc tcagctgctt tccgatcgct ttgatggcgt ttgcgtacat
    85561 cctgaaagtg aaatcgttca cgtatggcgc ggcgggttat ggtgtccggt cagcacaatg
    85621 gaacttagcc gcgaaatggt ggcgatctat tcagagcaca gggccacttt cagcaagcgc
    85681 gtaatcaata acgccgttga agcgttaaaa gttattgccg aaccaatggg cgagccgtcc
    85741 ggcgatttgt tgccgttcgc caatggtgcg cttgacctga aaacggggga attttccccg
    85801 cacacgccgg agaactggat caccacgcac aacggcattg agtacacgcc accagcacca
    85861 ggggagaaca tccgcgataa cgcgccaaac tttcataaat ggcttgagca cgcagccgga
    85921 aaagacccgc gcaagatgat gcgtatatgt gccgcgctgt acatgattat ggcgaaccgg
    85981 tacgactggc agatgtttat tgaggccacc ggagacggcg gaagcggtaa aagtacattc
    86041 acgcacatag ccagccttct ggcagggaaa cagaacacgg taagcgctga aatgacatcg
    86101 cttgatgatg ctggtggacg tgcgcaggtt gtcgggagtc gtcttatcgt cctggcagac
    86161 cagccgaaat atacaggcga aggaacgggc atcaagaaaa tcacgggcgg cgaccccgtg
    86221 gaaattaacc cgaaatatga aaagcgtttc acggcggtaa tcagggcggt ggtgctgacg
    86281 accaacaaca acccgatgat attcaccgaa cgggccggag gcgtggcacg tcgtcgggtt
    86341 atttttcgct ttgacaatat cgttagcgag gcagaaaagg acaaggattt gccggagaag
    86401 gtcgcggctg aaatccctgt gattatccgc cgattactgg cgaactttgc cgaccctgaa
    86461 aaggcacggg ctttactcct ggaacagcgc gacggtgatg aagcactggc aataaagcaa
    86521 cagacggatc cggttattga gttttgccag ttcctgaatt ttctggagga agcgcgcggc
    86581 ctgatgatgg gtggcggtgg cgattccgtg aagtacacga ccagaaacag cctttaccgc
    86641 gtctatctgg cgtttatggc atacgcagga agaagcaagc cgctaaacgt ggctgatttc
    86701 ag
//
LOCUS       DAZGCS010000003        81632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGCS010000003 DAZGCS010000000
VERSION     DAZGCS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.003"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1146
                     /gene="proV"
                     /locus_tag="ACHK01_000171"
     CDS             <1..1146
                     /gene="proV"
                     /locus_tag="ACHK01_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO8095184.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1139..2203
                     /gene="proW"
                     /locus_tag="ACHK01_000172"
     CDS             1139..2203
                     /gene="proW"
                     /locus_tag="ACHK01_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO8095185.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2261..3253
                     /gene="proX"
                     /locus_tag="ACHK01_000173"
     CDS             2261..3253
                     /gene="proX"
                     /locus_tag="ACHK01_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO8095186.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3545..4729
                     /locus_tag="ACHK01_000174"
     CDS             3545..4729
                     /locus_tag="ACHK01_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8095187.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4853..5590
                     /gene="ygaZ"
                     /locus_tag="ACHK01_000175"
     CDS             4853..5590
                     /gene="ygaZ"
                     /locus_tag="ACHK01_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO8095188.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5580..5915
                     /gene="ygaH"
                     /locus_tag="ACHK01_000176"
     CDS             5580..5915
                     /gene="ygaH"
                     /locus_tag="ACHK01_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO8095189.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLG
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6006..6536
                     /gene="emrR"
                     /locus_tag="ACHK01_000177"
     CDS             6006..6536
                     /gene="emrR"
                     /locus_tag="ACHK01_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO8095190.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHLLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6663..7835
                     /gene="emrA"
                     /locus_tag="ACHK01_000178"
     CDS             6663..7835
                     /gene="emrA"
                     /locus_tag="ACHK01_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO8095191.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7852..9390
                     /gene="emrB"
                     /locus_tag="ACHK01_000179"
     CDS             7852..9390
                     /gene="emrB"
                     /locus_tag="ACHK01_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO8095192.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9454..9969)
                     /gene="luxS"
                     /locus_tag="ACHK01_000180"
     CDS             complement(9454..9969)
                     /gene="luxS"
                     /locus_tag="ACHK01_000180"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO8095193.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(10119..11675)
                     /gene="gshA"
                     /locus_tag="ACHK01_000181"
     CDS             complement(10119..11675)
                     /gene="gshA"
                     /locus_tag="ACHK01_000181"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO8095194.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11748..12176)
                     /gene="yqaA"
                     /locus_tag="ACHK01_000182"
     CDS             complement(11748..12176)
                     /gene="yqaA"
                     /locus_tag="ACHK01_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO8095195.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12173..12739)
                     /gene="yqaB"
                     /locus_tag="ACHK01_000183"
     CDS             complement(12173..12739)
                     /gene="yqaB"
                     /locus_tag="ACHK01_000183"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO8095196.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13020..13096)
                     /locus_tag="ACHK01_000184"
     tRNA            complement(13020..13096)
                     /locus_tag="ACHK01_000184"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13060..13062),aa:Arg,seq:acg)
     gene            complement(13295..13371)
                     /locus_tag="ACHK01_000185"
     tRNA            complement(13295..13371)
                     /locus_tag="ACHK01_000185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13335..13337),aa:Arg,seq:acg)
     gene            complement(13435..13511)
                     /locus_tag="ACHK01_000186"
     tRNA            complement(13435..13511)
                     /locus_tag="ACHK01_000186"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13475..13477),aa:Arg,seq:acg)
     gene            complement(13709..13785)
                     /locus_tag="ACHK01_000187"
     tRNA            complement(13709..13785)
                     /locus_tag="ACHK01_000187"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13749..13751),aa:Arg,seq:acg)
     gene            complement(13789..13881)
                     /locus_tag="ACHK01_000188"
     tRNA            complement(13789..13881)
                     /locus_tag="ACHK01_000188"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13845..13847),aa:Ser,seq:gct)
     gene            complement(14197..14382)
                     /gene="csrA"
                     /locus_tag="ACHK01_000189"
     CDS             complement(14197..14382)
                     /gene="csrA"
                     /locus_tag="ACHK01_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO8095197.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14617..17247)
                     /gene="alaS"
                     /locus_tag="ACHK01_000190"
     CDS             complement(14617..17247)
                     /gene="alaS"
                     /locus_tag="ACHK01_000190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO8095198.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17375..17875)
                     /gene="recX"
                     /locus_tag="ACHK01_000191"
     CDS             complement(17375..17875)
                     /gene="recX"
                     /locus_tag="ACHK01_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO8095199.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18118..19179)
                     /gene="recA"
                     /locus_tag="ACHK01_000192"
     CDS             complement(18118..19179)
                     /gene="recA"
                     /locus_tag="ACHK01_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO8095200.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19259..19756)
                     /gene="pncC"
                     /locus_tag="ACHK01_000193"
     CDS             complement(19259..19756)
                     /gene="pncC"
                     /locus_tag="ACHK01_000193"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO8095201.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19901..20986)
                     /gene="mltB"
                     /locus_tag="ACHK01_000194"
     CDS             complement(19901..20986)
                     /gene="mltB"
                     /locus_tag="ACHK01_000194"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO8095202.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21242..21805
                     /gene="srlA"
                     /locus_tag="ACHK01_000195"
                     /gene_synonym="gutA"
     CDS             21242..21805
                     /gene="srlA"
                     /locus_tag="ACHK01_000195"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO8095203.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21802..22761
                     /gene="srlE"
                     /locus_tag="ACHK01_000196"
                     /gene_synonym="gutE"
     CDS             21802..22761
                     /gene="srlE"
                     /locus_tag="ACHK01_000196"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO8095204.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22772..23143
                     /gene="srlB"
                     /locus_tag="ACHK01_000197"
                     /gene_synonym="gutB"
     CDS             22772..23143
                     /gene="srlB"
                     /locus_tag="ACHK01_000197"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO8095205.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23147..23926
                     /gene="srlD"
                     /locus_tag="ACHK01_000198"
     CDS             23147..23926
                     /gene="srlD"
                     /locus_tag="ACHK01_000198"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO8095206.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24031..24390
                     /gene="gutM"
                     /locus_tag="ACHK01_000199"
     CDS             24031..24390
                     /gene="gutM"
                     /locus_tag="ACHK01_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO8095207.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24457..25230
                     /gene="srlR"
                     /locus_tag="ACHK01_000200"
                     /gene_synonym="gutR"
     CDS             24457..25230
                     /gene="srlR"
                     /locus_tag="ACHK01_000200"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO8095208.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25223..26188
                     /gene="gutQ"
                     /locus_tag="ACHK01_000201"
     CDS             25223..26188
                     /gene="gutQ"
                     /locus_tag="ACHK01_000201"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO8095209.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26185..27699)
                     /gene="norR"
                     /locus_tag="ACHK01_000202"
     CDS             complement(26185..27699)
                     /gene="norR"
                     /locus_tag="ACHK01_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO8095210.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27886..29325
                     /gene="norV"
                     /locus_tag="ACHK01_000203"
     CDS             27886..29325
                     /gene="norV"
                     /locus_tag="ACHK01_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO8095211.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29322..30455
                     /gene="norW"
                     /locus_tag="ACHK01_000204"
     CDS             29322..30455
                     /gene="norW"
                     /locus_tag="ACHK01_000204"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO8095212.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30681..32933)
                     /gene="hypF"
                     /locus_tag="ACHK01_000205"
     CDS             complement(30681..32933)
                     /gene="hypF"
                     /locus_tag="ACHK01_000205"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO8095213.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33086..33613)
                     /gene="hydN"
                     /locus_tag="ACHK01_000206"
     CDS             complement(33086..33613)
                     /gene="hydN"
                     /locus_tag="ACHK01_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO8095214.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33762..34772)
                     /gene="ascG"
                     /locus_tag="ACHK01_000207"
     CDS             complement(33762..34772)
                     /gene="ascG"
                     /locus_tag="ACHK01_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO8095215.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            35032..36489
                     /gene="ascF"
                     /locus_tag="ACHK01_000208"
     CDS             35032..36489
                     /gene="ascF"
                     /locus_tag="ACHK01_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO8095216.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            36498..37922
                     /gene="ascB"
                     /locus_tag="ACHK01_000209"
     CDS             36498..37922
                     /gene="ascB"
                     /locus_tag="ACHK01_000209"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO8095217.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     KITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38036..38455)
                     /locus_tag="ACHK01_000210"
     CDS             complement(38036..38455)
                     /locus_tag="ACHK01_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8095218.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38526..39280)
                     /locus_tag="ACHK01_000211"
                     /pseudo
     CDS             complement(38526..39280)
                     /locus_tag="ACHK01_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39323..39793)
                     /gene="hycI"
                     /locus_tag="ACHK01_000212"
     CDS             complement(39323..39793)
                     /gene="hycI"
                     /locus_tag="ACHK01_000212"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO8095219.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(39786..40196)
                     /gene="hycH"
                     /locus_tag="ACHK01_000213"
     CDS             complement(39786..40196)
                     /gene="hycH"
                     /locus_tag="ACHK01_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO8095220.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40193..40960)
                     /gene="hycG"
                     /locus_tag="ACHK01_000214"
     CDS             complement(40193..40960)
                     /gene="hycG"
                     /locus_tag="ACHK01_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO8095221.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(40960..41502)
                     /gene="hycF"
                     /locus_tag="ACHK01_000215"
     CDS             complement(40960..41502)
                     /gene="hycF"
                     /locus_tag="ACHK01_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO8095222.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41512..43221)
                     /gene="hycE"
                     /locus_tag="ACHK01_000216"
     CDS             complement(41512..43221)
                     /gene="hycE"
                     /locus_tag="ACHK01_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO8095223.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43239..44162)
                     /gene="hycD"
                     /locus_tag="ACHK01_000217"
     CDS             complement(43239..44162)
                     /gene="hycD"
                     /locus_tag="ACHK01_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO8095224.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44165..45991)
                     /gene="hycC"
                     /locus_tag="ACHK01_000218"
     CDS             complement(44165..45991)
                     /gene="hycC"
                     /locus_tag="ACHK01_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO8095225.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(45988..46599)
                     /gene="hycB"
                     /locus_tag="ACHK01_000219"
     CDS             complement(45988..46599)
                     /gene="hycB"
                     /locus_tag="ACHK01_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO8095226.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46724..47185)
                     /gene="hycA"
                     /locus_tag="ACHK01_000220"
     CDS             complement(46724..47185)
                     /gene="hycA"
                     /locus_tag="ACHK01_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO8095227.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47397..47747
                     /gene="hypA"
                     /locus_tag="ACHK01_000221"
     CDS             47397..47747
                     /gene="hypA"
                     /locus_tag="ACHK01_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8095228.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47751..48623
                     /gene="hypB"
                     /locus_tag="ACHK01_000222"
     CDS             47751..48623
                     /gene="hypB"
                     /locus_tag="ACHK01_000222"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO8095229.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48614..48886
                     /gene="hypC"
                     /locus_tag="ACHK01_000223"
     CDS             48614..48886
                     /gene="hypC"
                     /locus_tag="ACHK01_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO8095230.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48886..50007
                     /gene="hypD"
                     /locus_tag="ACHK01_000224"
     CDS             48886..50007
                     /gene="hypD"
                     /locus_tag="ACHK01_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO8095231.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50004..51014
                     /gene="hypE"
                     /locus_tag="ACHK01_000225"
     CDS             50004..51014
                     /gene="hypE"
                     /locus_tag="ACHK01_000225"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO8095232.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51088..53166
                     /gene="flhA"
                     /locus_tag="ACHK01_000226"
     CDS             51088..53166
                     /gene="flhA"
                     /locus_tag="ACHK01_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO8095233.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53203..53556)
                     /gene="ygbA"
                     /locus_tag="ACHK01_000227"
     CDS             complement(53203..53556)
                     /gene="ygbA"
                     /locus_tag="ACHK01_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO8095234.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(53588..53767)
                     /locus_tag="ACHK01_000228"
     CDS             complement(53588..53767)
                     /locus_tag="ACHK01_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095235.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            53843..56404
                     /gene="mutS"
                     /locus_tag="ACHK01_000229"
     CDS             53843..56404
                     /gene="mutS"
                     /locus_tag="ACHK01_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO8095236.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            56510..57166
                     /gene="pphB"
                     /locus_tag="ACHK01_000230"
     CDS             56510..57166
                     /gene="pphB"
                     /locus_tag="ACHK01_000230"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8095237.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(57217..57984)
                     /gene="ygbI"
                     /locus_tag="ACHK01_000231"
     CDS             complement(57217..57984)
                     /gene="ygbI"
                     /locus_tag="ACHK01_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO8095238.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            58180..59088
                     /gene="ltnD"
                     /locus_tag="ACHK01_000232"
     CDS             58180..59088
                     /gene="ltnD"
                     /locus_tag="ACHK01_000232"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO8095239.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            59085..60347
                     /gene="otnK"
                     /locus_tag="ACHK01_000233"
     CDS             59085..60347
                     /gene="otnK"
                     /locus_tag="ACHK01_000233"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO8095240.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            60344..60982
                     /gene="ygbL"
                     /locus_tag="ACHK01_000234"
     CDS             60344..60982
                     /gene="ygbL"
                     /locus_tag="ACHK01_000234"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO8095241.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            60987..61763
                     /gene="ygbM"
                     /locus_tag="ACHK01_000235"
     CDS             60987..61763
                     /gene="ygbM"
                     /locus_tag="ACHK01_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO8095242.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            61852..63216
                     /gene="ygbN"
                     /locus_tag="ACHK01_000236"
     CDS             61852..63216
                     /gene="ygbN"
                     /locus_tag="ACHK01_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO8095243.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(63255..63509)
                     /locus_tag="ACHK01_000237"
     CDS             complement(63255..63509)
                     /locus_tag="ACHK01_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO8095244.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRDT
                     EPLRRTSREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(63520..64947)
                     /locus_tag="ACHK01_000238"
     CDS             complement(63520..64947)
                     /locus_tag="ACHK01_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO8095245.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(64947..65540)
                     /locus_tag="ACHK01_000239"
     CDS             complement(64947..65540)
                     /locus_tag="ACHK01_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO8095246.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            65687..66094
                     /locus_tag="ACHK01_000240"
     CDS             65687..66094
                     /locus_tag="ACHK01_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO8095247.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(66215..67207)
                     /gene="rpoS"
                     /locus_tag="ACHK01_000241"
     CDS             complement(66215..67207)
                     /gene="rpoS"
                     /locus_tag="ACHK01_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO8095248.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67270..68364)
                     /gene="nlpD"
                     /locus_tag="ACHK01_000242"
     CDS             complement(67270..68364)
                     /gene="nlpD"
                     /locus_tag="ACHK01_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO8095249.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68549..69175)
                     /gene="pcm"
                     /locus_tag="ACHK01_000243"
     CDS             complement(68549..69175)
                     /gene="pcm"
                     /locus_tag="ACHK01_000243"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO8095250.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69169..69930)
                     /gene="surE"
                     /locus_tag="ACHK01_000244"
     CDS             complement(69169..69930)
                     /gene="surE"
                     /locus_tag="ACHK01_000244"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO8095251.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(69911..70960)
                     /gene="truD"
                     /locus_tag="ACHK01_000245"
     CDS             complement(69911..70960)
                     /gene="truD"
                     /locus_tag="ACHK01_000245"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO8095252.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(70957..71436)
                     /gene="ispF"
                     /locus_tag="ACHK01_000246"
     CDS             complement(70957..71436)
                     /gene="ispF"
                     /locus_tag="ACHK01_000246"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO8095253.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71436..72146)
                     /gene="ispD"
                     /locus_tag="ACHK01_000247"
     CDS             complement(71436..72146)
                     /gene="ispD"
                     /locus_tag="ACHK01_000247"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO8095254.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72165..72476)
                     /gene="ftsB"
                     /locus_tag="ACHK01_000248"
     CDS             complement(72165..72476)
                     /gene="ftsB"
                     /locus_tag="ACHK01_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO8095255.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72670..72993)
                     /gene="ygbE"
                     /locus_tag="ACHK01_000249"
     CDS             complement(72670..72993)
                     /gene="ygbE"
                     /locus_tag="ACHK01_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO8095256.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(73043..73648)
                     /gene="cysC"
                     /locus_tag="ACHK01_000250"
     CDS             complement(73043..73648)
                     /gene="cysC"
                     /locus_tag="ACHK01_000250"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO8095257.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73648..75075)
                     /gene="cysN"
                     /locus_tag="ACHK01_000251"
     CDS             complement(73648..75075)
                     /gene="cysN"
                     /locus_tag="ACHK01_000251"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO8095258.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(75077..75985)
                     /gene="cysD"
                     /locus_tag="ACHK01_000252"
     CDS             complement(75077..75985)
                     /gene="cysD"
                     /locus_tag="ACHK01_000252"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO8095259.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76237..77274
                     /gene="iap"
                     /locus_tag="ACHK01_000253"
     CDS             76237..77274
                     /gene="iap"
                     /locus_tag="ACHK01_000253"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO8095260.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77295..77934
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77417..77445
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(78031..78324)
                     /gene="cas2e"
                     /locus_tag="ACHK01_000254"
     CDS             complement(78031..78324)
                     /gene="cas2e"
                     /locus_tag="ACHK01_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO8095261.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(78324..79244)
                     /gene="cas1e"
                     /locus_tag="ACHK01_000255"
     CDS             complement(78324..79244)
                     /gene="cas1e"
                     /locus_tag="ACHK01_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO8095262.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(79241..79891)
                     /gene="cas6e"
                     /locus_tag="ACHK01_000256"
     CDS             complement(79241..79891)
                     /gene="cas6e"
                     /locus_tag="ACHK01_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO8095263.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(79873..80619)
                     /gene="cas5e"
                     /locus_tag="ACHK01_000257"
     CDS             complement(79873..80619)
                     /gene="cas5e"
                     /locus_tag="ACHK01_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO8095264.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(80630..>81632)
                     /gene="cas7e"
                     /locus_tag="ACHK01_000258"
     CDS             complement(80630..>81632)
                     /gene="cas7e"
                     /locus_tag="ACHK01_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO8095265.1"
                     /translation="SPKTVILGGATRLRVSSQSLKRAWRTSELFEQALAGHIGVRSGR
                     IAREAATILIEKGIEDKKAIEWAVEIADYLGKAKKDKKQKNDKKPKDPLTSAETEQLV
                     HISPAEFDAVKALAHQLAEEKRAPKEKDLALLRKDRMAVDIAMFGRMLAKKPGFNVEA
                     ACQVAHAFGVSETIVENDFFTAVDDLRQASEDAGAGHVDETGFGSALFYTYICIDKDL
                     LVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAYASWALAEKGTDQPRSLAA
                     AFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFDVMNKQGSMKDVLDFICA"
BASE COUNT        19060 a        22246 c        21109 g        19217 t
ORIGIN      
        1 cgagcgttca aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg
       61 ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc
      121 atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa
      181 cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa
      241 ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg
      301 catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa
      361 gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac
      421 agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta
      481 gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt
      541 cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc
      601 tttatttccc acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa
      661 aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat
      721 tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt
      781 gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca
      841 ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag
      901 tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt
      961 gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg
     1021 ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat
     1081 gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat
     1141 ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg cgcaatccgc
     1201 agacgcctgg ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac
     1261 cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct
     1321 gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc
     1381 cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcagttgct
     1441 gctgggtatg cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc
     1501 cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc
     1561 ctggtcgcag gcaatggtta ctctggcgct ggtattaacc gccctgctgt tctgtatcgt
     1621 catcggtttg ccgttgggga tctggctggc gagaagtccg cgagcggcga aaattattcg
     1681 tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat
     1741 gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc
     1801 gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc
     1861 gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat
     1921 gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat
     1981 tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct
     2041 ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga
     2101 tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac
     2161 cactggccct gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt
     2221 gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt
     2281 tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa
     2341 aggtattact gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct
     2401 ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg aagtggatta
     2461 caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac
     2521 gccactgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg
     2581 ggtatttgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta
     2641 caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt tcgataccaa
     2701 cggcgacgga aaagcggatt taaccggttg taaccctggc tggggctgcg aaggtgcgat
     2761 caaccaccag cttgccgcgt atgaactgac ccataccgtg acgcataatc aggggaacta
     2821 cgcggcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttacta
     2881 cacctggacg ccgtactggg tgagtaacga actgaagccg ggcaaagatg tcgtctggtt
     2941 gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac tgccgaatgg
     3001 tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctgggccga
     3061 gaaaaacccg gcagcagcga aactgtttgc cattatgcag ttgccagtgg cagatattaa
     3121 cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt
     3181 tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc
     3241 agcgcagaag taatttttat tcaggcggat aaggcgtttt tgcctcatcc gccgctctgt
     3301 acacaatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg
     3361 tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg
     3421 ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc
     3481 ccatattcat caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa
     3541 aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc
     3601 caccggtctg gcggtcgcca gtaactatta cgctcagcca ttgctcgaca ccatcgcgcg
     3661 taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta
     3721 tgcagcaggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat
     3781 tgtctcgatg accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct
     3841 ggcgatgatg atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct
     3901 ggttccgctg gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat
     3961 tatgagcggt ctgctgttgg ggatcttgct ggcacggaca gttgccggat tgctggcgaa
     4021 tctcggcggc tggcgcaccg tcttttgggt tgcttcggtg ttaatggcac tgatggcgct
     4081 ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt
     4141 gttgggttcc gttttcagta tgtttatcag cgataaaatt ctgcgtaccc gcgcgttgct
     4201 gggctgcctg acctttgcca atttcagcat tctctggacc tcaatggcct ttttgcttgc
     4261 cgctccacct tttaactaca gcgatggtgt aattggtctg tttggacttg cgggagctgc
     4321 cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgat aagggcaaat cgcaccacac
     4381 cacaactttc ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac
     4441 ttccgtactg gcgttgatta tcggaatcct ggtgctggat ctcaccgtgc agggcgtgca
     4501 tatcactaac cagacggtaa tttatcggat acatcctgat gcgcgcaatc gcctgaccgc
     4561 aggttacatg actagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc
     4621 ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa
     4681 cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa
     4741 ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta
     4801 agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag
     4861 ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca aagacagttt
     4921 accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct
     4981 gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca
     5041 gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tggattgctg cactgaccgt
     5101 catggcaatg gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca
     5161 gcgtctgcaa aaatcgaaaa ccgccctgtg ggcgtttggc ctgacggatg aggtttttgc
     5221 cgccgcaacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg
     5281 cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg
     5341 cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc
     5401 actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc
     5461 agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc
     5521 aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga
     5581 tgagctatga ggttctactg cttgggttac tggttggcgc ggcgaattat tgcttccgct
     5641 atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta
     5701 ttttgctcgg caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac
     5761 cagaagtaat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg
     5821 gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct
     5881 atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac
     5941 aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa tgaggtcata
     6001 cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc
     6061 acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca
     6121 agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta
     6181 tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt
     6241 gtgctcttgg gtcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg
     6301 gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg
     6361 aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatctac
     6421 tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc
     6481 tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg
     6541 tcatctcgct caaaaatcca gacttataaa agaaaaaatg actggccagc atcgcaacat
     6601 gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca
     6661 atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac
     6721 gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga
     6781 tttattggtt tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga
     6841 atcaaattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca
     6901 ccgattttgt aaaagaaggc gacgtgctgg tcactctcga cccgacagat gctcgccagg
     6961 cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga
     7021 ttaacagcaa acagttgcag gcgaatattg aggtgcagaa aatcgccctc gcaaaagcac
     7081 aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc
     7141 tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac
     7201 aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc
     7261 aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca
     7321 gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa
     7381 cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag
     7441 agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg
     7501 gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat
     7561 tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac
     7621 ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt
     7681 ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac
     7741 gttcaacacc ggtcgcggta agtaccgcgc gtgaaatcag cctagcacct gtcaataaac
     7801 tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag
     7861 caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca
     7921 ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat
     7981 ctgggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc
     8041 atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt
     8101 tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag cagcctgaat
     8161 atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat cccgctttcg
     8221 caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcact ggcgttgtgg
     8281 tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta tatcagcgat
     8341 aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc ggtggtgttg
     8401 atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg gattgatgcc
     8461 gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct cgaccgcggt
     8521 aaagagctgg actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg
     8581 gctatctgct tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg
     8641 ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc
     8701 tacttcggcg ctattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg
     8761 acctgggcag gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt
     8821 atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg
     8881 tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg
     8941 tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc
     9001 accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctccaac
     9061 tttacgcgta cgctggcggg gtctatcggc acgtccatca ccaccaccat gtggaccaac
     9121 cgcgagtcga tgcaccacgc acagttaacc gagtcggtaa acccgtttaa cccaaacgcc
     9181 caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc ctccggctgg
     9241 attgcccagc agatcaccaa ccaggggctg attatttccg ccaatgagat cttctggatg
     9301 tcagccggga tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccatttggc
     9361 gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc
     9421 ttttttcaat taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc
     9481 ttccggcagc gccagttctt cgttgctgtt gatgcgcacg tcacgttcca gaatgctacg
     9541 cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt
     9601 cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca
     9661 ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg
     9721 gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat
     9781 aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca cttctttgtt
     9841 cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt
     9901 tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa
     9961 caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca
    10021 ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg
    10081 aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac
    10141 accgcaaacg gttcggtatc agcggcttcc atttcctgct gacggcgttc agacgcctcg
    10201 cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg cagcagatta
    10261 cggtaggctt ctgcaaatgc tttgcctgtt ccgccaatac cagtatcaat catagacctt
    10321 aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag ttcatcacac
    10381 actttctgat acgcttcgcc gccgttaata ctatccagcg tttgcgcgac gcgtttcaga
    10441 tcgcggaaca gatctttacc cacctgcggt aacgggaact gtgcggtttc gcagccgata
    10501 cccagcgtca gacccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta
    10561 caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac
    10621 aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc
    10681 agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggcgactcg
    10741 ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca gttcgttttc aatctgcaac
    10801 acgttgctgt tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct
    10861 tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag
    10921 gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag
    10981 gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt
    11041 tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc
    11101 caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg
    11161 gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa
    11221 ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca
    11281 cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc
    11341 tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg
    11401 cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat atcaccatcc
    11461 actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc
    11521 agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc agcattaaca
    11581 cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc
    11641 agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg
    11701 ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac
    11761 atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt accaaggcac
    11821 aaaaaaaaga ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa
    11881 tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag
    11941 ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata
    12001 acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga
    12061 tgactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt
    12121 gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca
    12181 agcgaacatc cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat
    12241 cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg
    12301 tgtctggcgc gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac
    12361 ggcgtaatcc caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta
    12421 cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga
    12481 cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggt
    12541 cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat
    12601 taagcgcaat cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc
    12661 acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac
    12721 ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt
    12781 aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta
    12841 gttatttaga aatgagatat ttttgaagga aatttttggg agaagatggt gcatccggga
    12901 ggattactca ctggcgttcg cccttcgggc cgttgctgac gcaacgttat cctccctggt
    12961 gcctgcggtt aataccgcta tctcttcgat accttcattg ctgaaggtta cggagaatat
    13021 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
    13081 gctgagctac ggatgcatcg ggaaaattta ttttactgct gatattgata tcgctactaa
    13141 agccatatca agtaagagat ggtgcatccg ggaggattac tcgccttgcg gctcgccctt
    13201 cgggccgttg ctaaagcaac gttatcatcc ctggtgcctg cggttaattc cgctacctct
    13261 tcgatacctt cattgctgaa gatttcggag aatatggtgc atccgggagg attcgaacct
    13321 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa
    13381 atttatttta ctgctgatat tgataccgct actaaagcca tatcaagtaa gagatggtgc
    13441 atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga
    13501 gctacggatg catcgggaaa cttactttac tgcagatttt ttgataccgc tactaaagcc
    13561 gtatcaagta agagatggtg catccgggag gattactcgc cttgcggctc gcccttcggg
    13621 ccgttgctaa agcaacgtta tcctccctgg tgcctgcggt tataccgcca cctcttccat
    13681 accttcatcg ctgaaagtac gtagaatatg gtgcatccgg gaggattcga acctccgacc
    13741 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg gcggtgaggc
    13801 ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg ggagcgcctt
    13861 cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg
    13921 ctcatcgtcg ctgcgtggcg cacatattac tttctgagac ttataagtca aacaattttt
    13981 cctacacttt tatcgtttgc acacttcacg ttcaattagt ctgtaaaaac gtcaaaaaga
    14041 gtgttttatc aacagaaaaa tggaggtctg acagatagta gtaatgcaaa aaaatggaga
    14101 cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag aaattttgag
    14161 ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact gctgggattt
    14221 ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct tcggggcatt
    14281 tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga cctcatcccc
    14341 aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct ccttgaaaga
    14401 ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc caaaaagtaa
    14461 gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta aacctaagtt
    14521 tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta atgcgaatgc
    14581 cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg cgctgaccca
    14641 gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc
    14701 catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac caatcagttc
    14761 ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag aaaccttacc
    14821 ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta aatcgtcaac
    14881 catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca gcttaacacc
    14941 attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt
    15001 aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc gcactttatc
    15061 cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc
    15121 tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac gaacgcctgc
    15181 agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg
    15241 agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta cacgctcatc
    15301 atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga gatccatgat
    15361 gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg
    15421 aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt
    15481 aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg cagcgtgcat
    15541 caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat caacatcagc
    15601 ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc
    15661 ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt tcagttcgcc
    15721 tttatcacca acctgaccgc cggattccgc atagaatggt gtttgatcca gcacgaccac
    15781 agcttcctgg cctgcattga tggcatcaac cgctttacca tcaacaaaca gcgcggtcac
    15841 tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat caacacggat
    15901 cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc
    15961 cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac aaacatcagc
    16021 cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt caccatccag
    16081 cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc gctccagagt
    16141 acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct gctggcgttt
    16201 caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa
    16261 ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga tacgacgcag
    16321 tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga acgcacaaga
    16381 acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg cgccagtgac
    16441 tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag agttaacgtg
    16501 ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg gcttcggcag
    16561 cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt tccagatctc
    16621 aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa tgtggtcgcc
    16681 gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca tctgccagaa
    16741 gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac gttcgcgcgg
    16801 gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat agacggtaac
    16861 ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa
    16921 ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa
    16981 ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg
    17041 cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc caaggaacac
    17101 atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt tatggggtac
    17161 cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg
    17221 acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac gaaatatcgc
    17281 caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga ataagataag
    17341 ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt cgccaaatat
    17401 cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt
    17461 ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg
    17521 cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt
    17581 tctgattcag ttcctgacga atacgtgcag gtccataacc tttgcggcta cggctggcga
    17641 taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac caggcaataa
    17701 cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg cccataatcg
    17761 gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta
    17821 ccgcacgatc caacaggcga gcatatgccg ggcgacggga cgttgattct gtcatggcat
    17881 atccttacaa cttaaaaaag caaaagggcc gcagatgcga cccaggtttt gctgataaac
    17941 agatttaata gtcaatgagt gtgcagaatg ttcccgcagg cccaattcgt tagaggatcg
    18001 aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa ccctctcccc
    18061 aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa cgaacgatta
    18121 aaaatcttcg ttagtttctg ctacgccttc gctgtcatct acagagaaat ccggcgttga
    18181 gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg
    18241 gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct cacctttgta
    18301 gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc
    18361 gccgtagaag ttgatacctt cgccgtagag gatctggaat tcagcctgtt taaacggcgc
    18421 agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc
    18481 tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt
    18541 accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt
    18601 gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg
    18661 gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc
    18721 tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc
    18781 cagggcgtca cagatttcca gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt
    18841 atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat
    18901 aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt
    18961 tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc
    19021 cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg
    19081 gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc
    19141 tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg
    19201 taataccgga tagtcaatat gttctgttga agcaattata ctgtatgctc atacagtatc
    19261 aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac
    19321 gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca ccgcgggcag
    19381 tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg
    19441 cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgcg cctatcgcca
    19501 tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc
    19561 cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg
    19621 agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa gactcggcag
    19681 ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcactt aactgcatca
    19741 gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac
    19801 tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg
    19861 attagccaga gggaagctca cgcccccctc ttgtaaatag ctactgtact cgcgccagcg
    19921 ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg ttgtaacggg
    19981 tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca acatccagac
    20041 gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgcggcgg
    20101 caagctgcga aatgctgtac ttagttttga agccatttgg caagcctgga gcctgaccgt
    20161 ttgccattac cgcgacctga tcgcctttca cccagccgtg cgctttgaaa tagttcgcca
    20221 cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta
    20281 ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg
    20341 aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcaacagg aaggtttcca
    20401 gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat
    20461 cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga
    20521 cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact
    20581 gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac
    20641 gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat
    20701 ccatcagccg cagtaccgaa tccagacgtt tcgcctggga gagaatttcc tgcaactgct
    20761 gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat
    20821 tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag
    20881 acggcgttcc ggtggtcgta tcagtctcag taggttttgg cttgctgcta caggcggcaa
    20941 gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta
    21001 acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt
    21061 cactgcccga tacggacttt acataactca actcattccc ctcgctatcc ttttattcaa
    21121 actttcaaat taaaatattt atctttcatt ttgcgatcaa aataacactt ttaaatcttt
    21181 caatctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac
    21241 aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg
    21301 agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc tgctggttat
    21361 catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg
    21421 cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg
    21481 caatccgatg accctaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta
    21541 cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgg
    21601 cgaactgttt gtttatcttg gcattgccag cggtctgaca acgctgaacc tgccacttgg
    21661 cccactggcg gtgagttatc tgctggttgg tctggtcacc aatttcttcc gcggctgggt
    21721 gaccgatctg accaccgcca tttttgagaa aaagatgggc attcaacttg aacaaaaagt
    21781 tcacctggca ggagcaacat catgacgcat attcggatcg aaaaaggaac gggtggctgg
    21841 ggcggcccgc ttgagctgaa agccacgccg ggaaaaaaaa tcgtctatat caccgccggt
    21901 acccggcctg cgattgttga caaactggca cagcttactg gctggcaggc tattgacgga
    21961 tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc
    22021 acattacgct gcggcatcta tccgaaacga cgtattccca ccattaatat ccactcgacg
    22081 ggcaagtccg gtccgctggc gcagtacatt gtggaagata tttatgtctc tggcgtaaaa
    22141 gaagaaaaca tcactgtagt gggtgatgcg acaccacaac cctcttccgt gggccgtgac
    22201 tatgacacca gtaagaaaat caccgaacaa agcgatggtt tactggcgaa ggtgggaatg
    22261 ggcatggggt ccgccgttgc ggtgctgttt caatctggtc gtgacaccat cgacactgta
    22321 ttaaaaacca ttctgccgtt tatggcattc gtctcggcgc tcattggcat cattatggct
    22381 tctggccttg gtgactggat tgcccacggt cttgctccgc tggcgagcca tccactgggt
    22441 ctggtcatgc tggcgctcat ctgctccttc ccactgcttt cacctttcct cggcccaggc
    22501 gcagttatcg cacaggttat cggcgtattg attggcgtgc agattggtct cggcaatatt
    22561 ccgccgcatc tggctttacc ggcactgttt gccatcaacg cgcaggcggc ctgcgacttc
    22621 atcccggtcg gtttgtcgct ggcggaagct cgtcaggaca cggttcgcgt cggtgtccct
    22681 tctgtactgg tgagccgctt tttaaccggc gcgccgactg tactgatcgc ctggtttgtc
    22741 tccggtttta tctatcaata gaggctgaaa catgaccgtt atttatcaga ccaccatcac
    22801 ccgtatcggc gcgagtgcca ctgacgccct cagcgaccag atgctcatca cctttcgtga
    22861 aggcgcgcca gcggacctcg aagagtattg cttcattcat tgccacggcg agttgaaagg
    22921 tgaactccat cctggtttgc aatttacgct cgggcagcat cgctatccgg tgaccgccgt
    22981 tggcagcgtg gcggaagaca accttcgcga actgggtcat gtcaccctgc gcttcgatgg
    23041 tttaaacgaa gcggaatttc cgggcactgt ccatgtggca ggccctgtac ccgacgatat
    23101 cgcgccggga tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc
    23161 cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc tggctgccga
    23221 ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg tggcacaaga
    23281 aattaacgcc gaatatggtg aaggtatggc gtacggtttt ggtgctgacg ccaccagcga
    23341 gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg tggatttgct
    23401 ggtctatagc gccggaatag caaaagcagc ctttatcagc gacttccagc tcggcgattt
    23461 tgaccgttcg ctacaagtga atctggtggg ttatttcctg tgtgcccgtg aattttcgcg
    23521 tttgatgatc cgcgacggga ttcagggtcg cattattcag atcaactcga aatccggcaa
    23581 agtgggtagc aaacacaact ctggctacag cgcagcgaaa tttggtggcg tcgggctgac
    23641 tcaatcactg gcgctggatc tggcggagta cggcattacg gtgcattcac tgatgctcgg
    23701 taacctgctg aaatcgccga tgttccagtc actgttgcca caatacgcga ccaagctggg
    23761 tatcaaaccg gatcaagtcg agcagtatta catcgacaaa gtaccgctca aacgcggctg
    23821 cgattatcaa gatgtgctga atatgctgct gttctacgcc agtcctaagg cgtcgtactg
    23881 caccggacag tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat
    23941 ccgttaagga tctccgtgag actatagaat gcctgatgcg ctacgctcat caggcataca
    24001 ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt
    24061 atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc
    24121 ttcgacacac tatgccagca agggcgggtc ggcgtgggcc gttccagcgg gcgctttaaa
    24181 ccgcgggtcg tggtcgccat cgccctggac gatcagcagc gcatcgtcga caccttgttt
    24241 atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc ccgcaattac cggtatgcat
    24301 gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta
    24361 tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag
    24421 ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc
    24481 gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac
    24541 tttgacacca caggcacaac cattcgcaaa gatctggtca ttctggaaca tgccggaacc
    24601 gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat
    24661 cataaaacgc tcatcaacac ccacaagaaa gagctgattg cagaagctgc cgttagtttt
    24721 attcatgatg gcgactcaat aattcttgat gctggcagta ccgttttgca gatggtcccc
    24781 ctgctctcgc gctttaataa catcacggtg atgaccaaca gtctgcatat cgtcaatgcg
    24841 ctatctgaac tggataacga acaaaccatc ctgatgccag gcggaacatt tcgcaaaaaa
    24901 tcggcctcat ttcacgggca actggcagag aatgccttcg agcatttcac cttcgacaaa
    24961 ttattcatgg gtactgacgg catcgatctc aatgcgggcg taaccacctt caacgaggtc
    25021 tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttaat ggcggactca
    25081 tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg
    25141 attaccgacg caggtatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat
    25201 gtgatcataa ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt
    25261 aatgctggag ttgcaggaag caagccgttt accggaacgt ctgggcgatg attttgttcg
    25321 cgccgccaat atcatcctgc actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc
    25381 gggccacatt ggtaagaaaa tcgccgcaac acttgccagt accggcacac cggctttttt
    25441 tgtccatccg gcagaagcac tgcacggcga tctggggatg attgaaagcc gcgatgtgat
    25501 gctgtttatc tcttactccg gtggcgcgaa ggaactggat ctgattattc cgcgtctgga
    25561 agataaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc
    25621 ggcaaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc
    25681 gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca
    25741 ggcgcgcggt tttaatgaag aagattttgc ccgctcccac ccagctgggg cactgggcgc
    25801 tcgcttgctg aataaagtgc atcatctgat gcgccgtgat gacgctatcc cgcaggtagc
    25861 gttagccgcc agcgtgatgg atgcgatgct ggagctcagc cgcaccggtc tggggctggt
    25921 ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg
    25981 ctggctggtt ggcggcggcg cactcaccac gccagttaat gaagcgatga cgacaggcgg
    26041 caccacatta caggcgcaaa gccgtgccat cgacgccaaa gagatcctga tgaagcgcaa
    26101 aatcactgcc gcaccggtgg tagatgaaaa cggcaaactc accggcgcaa ttaacttgca
    26161 ggatttctat caggccggga ttatttaatc cttcaatccc agacgtttcg ccagccgatg
    26221 caggttggcg acgtcagttt ccagcattcg cgcgcaggca gcccagttgt gatgattttg
    26281 tgccagtgcc tggcgaatgg tttcacgctg gaacgcttct gtcgcttcac gcagattttg
    26341 cttaacaacg ggcaccgctg ccgcttctgg cggcggcaac gtcacctcag gaaaagcaaa
    26401 atgttgcgcc tcaagaatca cttcatcgcc gctgcgagtg gctctcgaca gtactaccgc
    26461 ccgatgaata gcatgttcca gttcgcgcac gttccccgga aaactgtagt gttgcagtaa
    26521 atttcgcgcc ccggcactta ataccacgcg ggagagcccc agccgcaaac gacactgctc
    26581 gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga
    26641 aagtggaaac acgctcaggc gatgaaacaa gtcagcgcga aatcgccctg ccagtacctc
    26701 ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aactgcggtc
    26761 atcgccaacg cgctgaatat cgccatactg caacactcgc agcaacttgg cctgcaaagc
    26821 caacgacaac tcgccgatct catcgagaaa cagtgtgccg ttatccgcca tttcaaactt
    26881 cccgctgcgg ttactgatag cgccagtaaa cgcccctttc acatgcccga acagttcact
    26941 ttccgccaca ctttccggca gtgcggcaca gttgagatag atcagcggat tcaccgcccg
    27001 tggcgaggct tcatgaatcg ctttcgccac cagctcctta ccggttcccg tctcaccgct
    27061 gattaagacg ttgagatcgg acgccgccac aatctcaatc tcttttttca attgcgtcat
    27121 gcctggcgat aagccaatca tctgcgtctg tttcaccgct tcaaacggcg cggcatcgcc
    27181 cggcagcata ttctggcttt ccagttgctc aatcagcaac gcattgctta acgctcccgc
    27241 cgccagcgca gcaatcagcc gtaactcttc atcgctgaaa acatcgaact gatcgggctg
    27301 catcccgtcg agcgtcaatg cgccaatcag gttttgcccg gcaaacaatg gcagaccaac
    27361 gcaggcgtga accttcagac tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa
    27421 ttcgctgtct gcaggaaagc gcaccacgtc cccggcgcgg gcaatcgctt caagccgtgg
    27481 atgcccttcc agcgcaaagc gtctaccgag tacatccttt gccagaccgt cgatggcaag
    27541 cggaataaac tgccgcgaat cgtaacgtag caacgcagac gcatcgcact ccagcacctg
    27601 acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga ccaatcccac gctgcaattc
    27661 gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg
    27721 actatagata ttgtcatatc gaccatttga ttgatagtca ttttgactac tcattaatga
    27781 gcatactttt atttatatag taaaaacaat cagataaaaa actggcacac aatctgcaat
    27841 tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc tattgtggtg
    27901 aaaaataaca ttcattgggt tggtcaacgt gactgggaag tgcgtgattt tcacggcacg
    27961 gaatataaaa cgctgcgcgg cagcagttac aacagctacc tcatccgcga agaaaaaaac
    28021 gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat
    28081 gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcagaaga ggaccacgct
    28141 ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc cgatctactg tactgccaac
    28201 gctatcgact cgataaatgg tcatcaccat catccggagt ggaattttaa tgtggtgaaa
    28261 actggcgaca cgctggatat cggcaacggc aaacagctca tttttgtcga aacaccaatg
    28321 ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct gttcagtaac
    28381 gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt ggatcagacg
    28441 gagcttttcg agcagtgcca gcgttactac gccaatatcc tgacgccgtt cagccgcctg
    28501 gtaacgccga aaattaccga gatcctgggc tttaacttac cagtcgatat gatagcgacc
    28561 tcccacggcg tggtctggcg cgataacccg acgcaaattg tcgagctgta cctgaagtgg
    28621 gccgctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc gaataacacc
    28681 cgcatgatgg ctgacgctat cgcccaggga attgcggaaa ctgacccacg cgtggcggtg
    28741 aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt cttccgctca
    28801 aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa aatcgccggg
    28861 ctggtggagg agatgactgg tttacgcttc cgtaacaaac gcgccagtgc tttcggctct
    28921 cacggctgga gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc
    28981 gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc aggacgctct ggagttatgt
    29041 cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg
    29101 gtgaatacgg tggttaaaga agaaacctct gccaccacga cggctgacct cggcccacgg
    29161 atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc aatgcaggac
    29221 gttgcgccag gaacgccgtg gagtgaagtt ccggataact tcctctgccc ggaatgctcc
    29281 ctcggcaaag acgtctttga agaactggca tcggaggcaa aatgagtaac ggcattgtga
    29341 tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca
    29401 ctattccatt aaccctgatt gccgccgaca gcatggatga gtacaacaaa cctgacctca
    29461 gccatgttat cagtcagggg caacgtgccg acgaccttac ccgccagacg gcgggtgaat
    29521 ttgccgagca gtttaatctg cgcctgtttc cgcacacctg ggtgacggat atcgatgccg
    29581 aagcccgtgt ggtgaaaagc cagaataatc agtggcaata cgacaagcta gtgctggcaa
    29641 ccggtgccag cgcctttgtc ccgccagtgc ctgggcgtga gttaatgctg acgttaaata
    29701 gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg
    29761 tcggtggtgg cttgattggt agcgaactgg cgatggattt ttgtcgtgca ggcaaagcgg
    29821 tcacgctaat cgacaacgct gccagtattc tggcgtcatt aatgccaccg gaagtaagca
    29881 gccgcttgca gcatcggttg acggagatgg gcgttcatct gttgttgaaa tctcagttgc
    29941 aggggctgga aaaaacagat tctggcattc tggcaacgct ggaccgccag cgcagtatcg
    30001 aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aaccgccctg gcacgacgcg
    30061 ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg
    30121 atatttacgc gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg ttcctccagc
    30181 cgattcaact tagcgcgatg gtgctggcaa aaaatcttct cggcaataac acgccactga
    30241 aactaccggc aatgctggtg aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg
    30301 aaacccagcg tcaggattta cgctggcaga ttaataccga acgccaggga atggtggcgc
    30361 gcggcgttga cgatgctgac cagcttcgcg cctttgtggt cagtgaggat cggatgaaag
    30421 aggcatttgg attgttgaaa acgttgccga tgtaggtggg ctactgtgcc taaaatgtcg
    30481 gatgcgacgc tggcgcgtct tatccaacct acggggagcg catgtgttgg tcacatactg
    30541 cgttcatgtc gcatccacct actgtgccca aaatgtcgga tgcgacgctg gcgcgtctta
    30601 tccgacctac ggggacgcat gtgtaggccg gataaggcgt ttacgccgca tccggcaatg
    30661 gtgtccaaat gcaacacgtt ttatccgttc tggacttcac ccgctaacca acgcgccgca
    30721 gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag
    30781 agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata
    30841 accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac
    30901 gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccggtgcc
    30961 tgccagttca gccactgctg ccagaaagtg gcgagatcca gttgattgtc caccagcggc
    31021 attgtcaccg gatgcgtcac tccgtggcac gaagctgcga gtgcctccag agcacaagcc
    31081 gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc cacggcatcg
    31141 aacaaccgcc cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc
    31201 agcacgctcc agttttgctg ttgcacactt gctgtttcag aataattctg ccactccggc
    31261 acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcgtcgccgc caaatcgcca
    31321 cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc
    31381 agacactcgc cgccccacaa agcgccgttc tcccccatac cgataccatc gagcgtcaaa
    31441 gcaatgacat caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcgtgg
    31501 gcatgatgat gcagcaccgt ttgcgtcggc atattcattt cgctcgccca ctggctggag
    31561 acatagcccg gatgcacgtc atgcacaacg tattgcggag tgaaatcgta gatgttttgc
    31621 atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga
    31681 tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtgttttt cagatccgcg
    31741 ccaagacaca gcacaggcgg aacattttta aagcccggag gcaaaaccag cgcatccggc
    31801 acatacccgc gtgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc
    31861 cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc
    31921 agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga ggtcatcacc
    31981 agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacattacc
    32041 ccgacttcgt taaggtcagg ggcgatatca tcacaaagct caggaacgta ttttttatcc
    32101 accagcacaa tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc tggtaaaccg
    32161 tcagccactg gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg cgcccgcagt
    32221 gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg
    32281 atggcgacaa tgttgcccat ttttaactgt gcgatagccg cctgtaatgc cgcctcttgt
    32341 tctgcatgtt caccatgact tacccattca agatgtgggc cacactccgg gcaggccacc
    32401 ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact ctttgtcaca agccggacac
    32461 aacggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg
    32521 aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg ttcgcctggg
    32581 gtattcattt cggcaagaca ggcagggcaa gtagcggcat cgggaacaat ttgcgtattc
    32641 atggtgccgc ctgtgctctg gcgtatagtg aactcggtgg gcagttgtga ccagatgtac
    32701 ggctcacgct cgacgctatc aatacgcgcc agcggcgggc agtgctgatg caattgaaca
    32761 agaaacgttt ccgggtcttc cagcagccgg acttctacgc catcgccgtc attacagaca
    32821 tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc
    32881 tgcactttgc ctcgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta
    32941 ttcccgccat catgaattgc gtaacccgcc ctgccggaca cgactgcgtc gtatccggca
    33001 gtctcaggtc ggcgataccg ccagctccgt attctacgaa tatttccggg aattcctttg
    33061 atgccagaac agttctataa gatttttaga acatcagcgc cgtacggcga cgtttttctg
    33121 cgctcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgggtc gggcaagccg
    33181 ccatacacgc cgggccgtct tcgcgatggt tgcacaggtc gcatttattg gcttcagctt
    33241 tgtcagcccg tacattcagc cccgcgccgc tgttgcggat caccggacgt accaccactt
    33301 ccatcgcgcc atacgggcaa gccaccacgc aggttttgca accaatgcaa cgttcctgca
    33361 tcacatgaac aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg
    33421 gtgcatcttc acactgacgg cagactgtcg ccgtggaaat gttcacacct ttaatgacat
    33481 ggattcgcgg taaaaaggtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag
    33541 aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga
    33601 tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc
    33661 atgccagttt ttaatttgct gttatttaag gaaattaatt tctgtaatgc aggaaaaaag
    33721 atgtcatcga cactagtgac gatgacatgt gatgacaatg tttatcgcga aagggcaatg
    33781 agtgagtcgc ggcggatcag ttttccgctg aaggttttcg gcggtgagaa atccccgcca
    33841 tcgagcataa aaatcagccg tccaataatt tcctgaatca tctctgttac cggaattttt
    33901 acgctggaga gcgccggaac ggtgtagggg gcaattgcga tatcatcgaa tccgataact
    33961 gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc
    34021 atatcgtcgt tactggcaac taacgcgcta aatttagccc cacgttcgag caacgtttct
    34081 accccttcgg ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc
    34141 gcaataccat gctgcgccag cgcgtcttta tacccagcaa gacgttcaat gctggtgggg
    34201 gaatccatcg agccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc
    34261 acggcattaa agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc
    34321 aggcggcgat taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcgatctca
    34381 tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac
    34441 tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgccgc
    34501 cctttctctt ccgccattcg cgcggcatga aagagtaatt cactaaaata aatgccgtgg
    34561 taaagcgtgt tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgc
    34621 gccagcaagt ttgggcggta accgctctct tctaccgcct gaaacacgcg atctttagtc
    34681 tcctggctga cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg
    34741 gcgcgcttcg ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac
    34801 gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt
    34861 gaccggtttc acaaatataa aaaatgaaca attaactctc ttgcttattt agtgacaact
    34921 attcatgatt ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtta
    34981 cgtcgactga taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa
    35041 aattatgcgg cgctggcacg ctcggtgata gcggcactgg gcggcgttga taacatctcg
    35101 gcagtcacgc actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac
    35161 agcccgacgt taaaaaccat ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt
    35221 caggtgatta tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg
    35281 ggagatatgc agcccgcaca gcccgtgggt aaacccaaac tcacgctgcg tcgcattggt
    35341 gcggggatcc tcgatgcgct gatcagcacc atgtcaccgc tgatcccggc gattatcggc
    35401 ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga gcggcgtgtt gacaaaagga
    35461 tcgccgacct taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgcta
    35521 atggtcgcgg cttcggccgc catcaaattt aaaaccaata tgtcgctggc gattgccatc
    35581 gcaggtgtgc tggtacatcc gagctttatt gaactgatgg cgaaagcggc ccagggtgaa
    35641 catgtcgaat ttgccctgat tccggtcacc gcggtgaaat atacctacac ggtgatcccg
    35701 gcgctggtga tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg
    35761 gctgtgacca aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca
    35821 atcctgctga ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac
    35881 accattcatg gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg
    35941 ctggtaatga ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa
    36001 accggcaaag aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt
    36061 tcatcactgg cggtggcgtg gaaaacgaaa aacccggaac tgcgccagac ggcgctggct
    36121 gcggcggcat cagccattat ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc
    36181 cgcctgaaac gtccgcttat cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc
    36241 ggtatggcgg ggcttgccag ccactcaatg gcagcgccgg ggctatttac cagcgtgcag
    36301 ttcttcgatc cggcgaatcc aatgagcatc gtctgggtat tcgcggtcat ggcgctggcg
    36361 gtggtgctgt cgtttatcct cacactgttg ctcggctttg aggatattcc tgttgaggaa
    36421 gcgactgccg aggcgcgaaa gcatcagagc gtacaaccga ccgtcgccaa agaagtaagt
    36481 cttaattgag gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg
    36541 ccgccaacca gtctgaaggt gcgttccgtg aaggtggcaa agggctgacc actgtcgata
    36601 tgatcccaca cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt
    36661 tgcgcgatga cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag
    36721 aagatatcgc cctgatggca gagatgggat tcaaggtttt ccgtacctca attgcctgga
    36781 gccgcctctt tccgcagggc gataaaatca cgcccaatca gcagggcatt gctttttacc
    36841 gttctgtctt tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact
    36901 tcgatgtgcc gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg
    36961 agtttttcag ccgctacgcc agaacctgct ttgaagcatt tgatggtctg gtgaaatact
    37021 ggctaacctt caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg
    37081 tgtttgaaga aggtgaaaat caggatcagg tgaaatatca ggccgcgcat caccagctgg
    37141 ttgccagtgc gctagccacc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct
    37201 gtatgctggc gggcggtaac ttctaccctt acagctgcaa accggaagat gtctgggcgg
    37261 cgctggagaa agatcgggaa aacctgtttt ttatcgatgt gcaggcgcgc ggcgcgtatc
    37321 cggcttactc tgcccgcgta tttcgcgaaa aaggggtaac catcaacaaa gcaccgggcg
    37381 atgatgaaat cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcgcgct
    37441 gcgcctcggc ggagatgaac gccaacaaca gcagtgcggc gaacgtggtg aaatcgctgc
    37501 gtaatccgta tctacaggtg agcgactggg gctggggaat tgatccactc ggtctgcgta
    37561 tcaccatgaa tatgatgtac gatcgttatc agaagccgct gtttctggtg gaaaacggcc
    37621 tcggcgcaaa agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct
    37681 atttacgcga acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg
    37741 gctacaccac ctggggctgt attgatttag tttccgcctc tacgggtgaa atgagcaaac
    37801 gctacggttt tgtctacgta gaccgtgacg acgcaggcaa cggcacgctg acgcgcacgc
    37861 gtaagaaatc gttctggtgg tataaaaaag taattgccag taatggggaa gatttagagt
    37921 aagtaaccgt gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccat
    37981 gaaggcccga taagcgcagc gcatcgggca atctggggtt tggcatcagt caaaatcaaa
    38041 gaaaccattc cggcttaacg ccaaatcggg ctgaaagtgc cttaatgtgg gatatcgtca
    38101 gggatcgttg tccagacaaa atctgactga ccagggattt agagccgatt tcgttcttca
    38161 aatccgcata ggtcagatta tgttgatcaa tcaacgtgcg aagtaacgcc acgccaaccg
    38221 gcattgccgc aacggctttg tcgaactctg caaacttttc gttgttgttt tcatactctg
    38281 caatgcggct cgccagaaag tcgataagtg gattggtgtc atcttcttca atcaaatatt
    38341 cgaccagttc cagtgcttca cgataatcat cttctgagtc actgcctccc agaaagggga
    38401 ctgcattgac cagttctctt gttgctttaa cagcgcgtgc agcattagca gtcattcttt
    38461 gttctctctg tagtaacgtg acgtctgtct gcacgtaatc cccctcatag ccactctgca
    38521 aaaagtcagt tctggttcac cccataaagc acatccccct ttatcaagca acgctgcgtc
    38581 tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg gtttgaggac gagtttacgg
    38641 ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta acttgccaaa ccagccgtga
    38701 acacgctgcc atttggcgtc ttttaccgat acagactggt tatccagtaa ccggaatcaa
    38761 actgcatatc cagcaattgc gtgtggccat tcctttgtaa atccagcaaa tcatcaatgc
    38821 cagtttcact tgcggtataa aaaccatcgg actgaaaaac acacgggcga ccttgctgat
    38881 taaaggtcat cagaatcaaa aacgcgttag gtgccagacc atttcctgaa ataccccgcc
    38941 agataaccag atcctgaatg ccgttacgat caagatcggc acggtaaagt tgcgcattac
    39001 ccgccatatt ttgcagcact cccacaggca cagaccagga tttaccggcg cgatctttac
    39061 cgttcaattg ccattcacta tcatcatttg gcatcttttc aagcgtcacg ccagcaacgc
    39121 cgacttttct aaaatgccag ttttgctcgg catcataagg catggtcagg agaggtttca
    39181 gcgcccgctt ttgcgcgtat tcgctgtcac tcattggcag atattcggta aagctggtgg
    39241 cctgtgcagg cagcactaag ctcaataaaa aagcgcacat ccctgttctg atgttcaatt
    39301 ccctgaattc cctgatggag aattattctt cttccaccgc taactgcgca aatccaccat
    39361 ttccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctggg
    39421 tcatcgggta gtaaaagccg acgatatccg gctgaatgcc gaggaaaatc acttcaccaa
    39481 tgtcttcttt taactggtcg ataaggtaat tcaacggcat gttatgggta gtcatcataa
    39541 acatctcggc gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat
    39601 ccgtggcgtc gacaatcagc agtcgtgtcg ggcgcagttc acggatggcg acgatgtcgt
    39661 tttccggtgc actaccgccg tcaatcacca cccagttacc tttcggcgcg gcggcgcact
    39721 tttccgccag cagcgggcca gcgccatcat cgcccatcat gctattgcca acacagagta
    39781 aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca
    39841 tgcagcatac ttaacagcat tgtactccat tccttctgct gcgaagtttg cgctgcccgg
    39901 gctttatcga atgcgttggc cagcatcggc acatggttaa tgtcgatgac gatctcgcca
    39961 tatttcggta cgccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc
    40021 catgccagat attcatccca tgggcaggtg agcgccgctt ccaggcaatc gataacccca
    40081 aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca
    40141 tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga
    40201 tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt
    40261 tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtcaggaagt
    40321 catcggcaat ctgacgaccg taacgataac ctgccaggcg gcgtgcttcg cgatccactt
    40381 tcacacgcag cggctgcacc atatcgccat gcaggatttc cgccggttgt tcatcctgct
    40441 cacccgggcc acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt
    40501 acagcgtggc ggcaggcgtt ggcgggcaac cagggatgta gacatctacc gggacgattt
    40561 tatccgtacc gccccacacg cagtagagat cgtggaagat cccgccactg ttaccgcagg
    40621 caccgtagga gatacagatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg
    40681 atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag
    40741 gaacgacttt aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg gcgaaaattt
    40801 cgatttcgca gccgttgcag ccgccgcagt ccacgcggta aacgtaggca gaacgtttga
    40861 tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg
    40921 gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg
    40981 gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca
    41041 agtctcaaag ctttcgcggt ggttttccgc gcggctgtcg ccgttgtgct taagtagcgc
    41101 aatggcgtag tcgatctctt tctggacagc gaaaggacga ttgcagacgc gacagttgca
    41161 cagcgcgaag cgggactgtt gcaggaagtc ttctttcttc cacaccgcca gttcgtactc
    41221 ttgcgacagt ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca
    41281 gcgtccaaga ttgaactccc acgcaagctc tcctgtggcg aggtcggttt caaccgttaa
    41341 ggcgtttgac gggcaggcat tgacgcaggc cgcgcagccg atgcactgct gcgggttctg
    41401 ctctggctta ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt
    41461 cgccgtgccg gttttgataa cttttttgat aaaggtaaac atggcgattc cttatttcag
    41521 cggcgagttt ttacgctcaa tgctgtagcg ctcgagttct ttgtacggca ccactttgct
    41581 cttcttctta cgtacatcga ccaccgtcat gcggtcggta caggagtagc aagggtccag
    41641 gctaccgata atcagcggcg catcggaaac ggtattgcca cgcagcatgt agcgcagggt
    41701 cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc
    41761 ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg
    41821 gtgcggaatg taggtaaagc cttccaccat cagcggaccg cccggcaggt tatccagacc
    41881 gtagtcgatc atgttcagcg cggtataaac ttcgttgata cgcactttca gacgggaaat
    41941 aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg
    42001 gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga accatcgggc caacgttact
    42061 gaaatcgcgg gcgatttccg ggtccagacg accaatgccg acggtgcgct gttccatgtt
    42121 cggcgtactc agcagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag
    42181 ctggcgggtc tggatcatgt catctttcaa cagatcgcga cgaatcccgc cgatcaggtt
    42241 caggccgtag gttttacgcg caccggtgag gatctctgcc attttcatgg aggtttcacg
    42301 cacgcggaag aactgcataa agccggagtc aaagccggta aagtggcagg cgagaccgag
    42361 gttgagcaga tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc
    42421 acgttctggc acctgaatac ccatcgcgtt ttctaccgac gtggtgtagg cagtgctgtg
    42481 ggcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat
    42541 acgggtttcc gccagttttt ccatgccgcg atgaacgtag aacagacggt agtcggcgtc
    42601 gataatgttt tcgccatcga cgaacagacg gaagtggccc ggttcgtcag aagtgacgtg
    42661 cagcggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta
    42721 ggtttcagca tcggtagtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag
    42781 cggataaagt tcatccggcc agtcatccgg cagcaccaga cgacgttcat ccggcagacc
    42841 aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac
    42901 gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac
    42961 ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    43021 tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa
    43081 ctccaccact tccggcaggt agttcacctt cacggtcacg gtcagctgat ctttggtctg
    43141 ccaggcgtgg tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg
    43201 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    43261 aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc
    43321 agacgggcca tgctgttttc gaacagcgcg ataaccagga cgccgacgac cagtttgacg
    43381 atggcaatca ccagcgccag cagcagccca ccggcggtga aggtttccat ctgtccccac
    43441 gggataaata cgccgacgaa catctgcagc accaccagct gtttcaggct gataccccat
    43501 ttcatgacgc caaagccgct gccgctgtat tcagagagcg ggccttcctg cagctcctgc
    43561 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaggtggc gaacgcacag
    43621 gcacaaagcg ccagtaccag cgggatgctc tggctcagcg gccagtgata aacggtgtcg
    43681 gtgatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc
    43741 agcatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcgccgat agcggtaaac
    43801 gggctaccgg tatccagacc agaaatggca aagaagaaac gcgcgatggc aaagagatac
    43861 agtaaggtga ttaaatcacc cagttgcggc agcggagaac cgacggtcac caccggcagc
    43921 gcagtagcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag
    43981 ccggaggcat ccgggccaac gctctggcgg cccagcagtt tgataatgtc acgatactcc
    44041 tgcaacacac ccggcccacg acggttatgt aagcgggctc gcgccacgcg ggtaataccg
    44101 gagagcagcg gcgcaacggc aaatagcacc agcgcctgaa ttaacggata taaaacactc
    44161 attctcaggc tcctcgtgaa acaataatca ccaccagcac cgccagttca accagcgcca
    44221 tccggcggaa cagcaacgca ctcccctcgc actgccagcc cggcaccaga gacaccggat
    44281 tcagccattt gcgtagtttc agcaccggcg caaacgcctg tttcaccggc atggcaaaac
    44341 cgtgagcggt aatcaccatt gatttttcgt ggtcgtagcc gcacacccag gccgcaccgc
    44401 gggaacgcga cggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg
    44461 ggcaggcaat cagcagcaac gtgatcatcg gttgagaaac ggtggtgtta gcaggctcca
    44521 gcggcagagg tacagcagca gagagcatcg gcagcagcca cggcgcagca acaccgccaa
    44581 ttacgcagca aatcgccagt gccactacgc ttacgctcat caggagcggc gcacaggtgg
    44641 cgttttcggc ttctttggta cgcggcgcgc cgaggaacgt gacgccatag actttcgcca
    44701 tacacatcac cgccagcgca ccggtaattg ccagacccac agcgagcagc ggccccagca
    44761 gacgggcaac aaacgcgcca ctattgctca gtttgaaaaa ggattgatag ataacccatt
    44821 ccccggcaaa cccgttcagc ggcggcagcg cagccattgc catcaacccg actaacatgg
    44881 cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac
    44941 cggtacggaa ccagacgctc cccgccccga ggaacagtac gcttttgaac aggctatggt
    45001 taagcagatg gtacagacca ccgaccagac caagggcaat cagcgccggt tgttcgagcg
    45061 cgatacccgt tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt
    45121 ggtaagccag caggcgctgg atgttgtgtt ccatcaacgc atacagacca ccgacgaacg
    45181 cggtgatcat gccgagcacc agcagcgcga tcccccacca cagcggcgcg ttaccaccca
    45241 gcagcgacag ggttaaaatg cccagcaggc caattttcat gactaccgta gaaaacaacg
    45301 cggcagctgg cgcagaggcg ttagcatgtg cctgcggcac ccagccgtgc agcggaataa
    45361 tcccggccag caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca
    45421 gctgttgcat acgcatatcc agcaggcgca gatccagcgt gccataacgc tgccacagca
    45481 gccagcaagc aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc
    45541 cctctttgct gttgctggtg aggaacaccg cacacaaggc catgacttcg gccattacca
    45601 cgaacatgcc gaggttgctg gcaatgacgg cgcagacggc ggcagccatc aacatattga
    45661 tctgcaagcc gttgcacttc acctgcgcgt ggcgatgcca gtcaatgtta tagaggctga
    45721 caaacagacc gcaaaggccg agggtaatca gccaaatcgc gttaagcggg gagatttgca
    45781 catcgtagct taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agcacagtga
    45841 agcccgcggc tgccgtatac aggctaccaa ccgcgccgcc aattccggct atccagccac
    45901 ttagcgtctt ttgaaaagaa aagagaaatg ccagaacagc ggcggcaata aaccacgcca
    45961 cgccgctatt gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa
    46021 caaggtgaga tcgccaaagt ccgtattaaa agtcagctca cgcttacgtt tgctgacgcg
    46081 ggcgatatcg gtgttatcca ccagatgcag ggctttggtc gggcacatcc gcacgcaggc
    46141 cggaccttgt tcatcaaaac tgcaaaggtc acatttgacg gcgatcgcgc gaatacccgg
    46201 cacccagtca agcagcgtac tgatacgcgc cggagcaggc ggtgccggtg gcgctttcgg
    46261 ggtattggca tttgcaggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa
    46321 cgggcaggcg atgccgcaca gcttgcagct tacgcacagg ctttcattca actgcacggc
    46381 cccatcaacg cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca
    46441 gtggtgacag agctgcggcg cagattcttt ttcatttagc atcactctca ggcgcggcat
    46501 tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag tgtggcagcc
    46561 gatacagagc gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg
    46621 tcatttttga cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat
    46681 ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt
    46741 gtccctttac cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc
    46801 tgtcgtaaac gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga
    46861 tgttgacctt gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt
    46921 tgtcttcgcc atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg
    46981 ccagggtgac gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg
    47041 cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat
    47101 tgcagtagat gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt
    47161 tctcgcttat ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt
    47221 aagcattttt tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat
    47281 gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga
    47341 taatcaacac tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc
    47401 acgaaataac cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg
    47461 gcgcaaaacg cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca
    47521 gctctcttgc cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac
    47581 tgcacctcga agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc
    47641 tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg
    47701 cagacgacgg tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa
    47761 catgcggttg cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg
    47821 cgtttcgtag cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta
    47881 aagcgcctga atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg
    47941 aagcgggcac tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg
    48001 tgctggacaa aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc
    48061 aactggtgct caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa
    48121 ccttaatgcg cctgaaagac agcgttccgt gcgcggttat tgaaggtgac cagcaaaccg
    48181 tgaacgatgc cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg aacaccggta
    48241 aaggctgcca tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg
    48301 ataacggtat tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc
    48361 tcggtgaaaa acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga
    48421 aatatccgca tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc
    48481 cgtatctcaa ctttgacgtt gaaaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa
    48541 ttgaaatcat ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc
    48601 tggagacaca gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca
    48661 ggcgaaagtc gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg
    48721 cgatgaaaac ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat
    48781 gagcgtaatt aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga
    48841 cgttgagccg gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat
    48901 gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca
    48961 catctctctt acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc
    49021 cacgctatct ttaaattcgg cctcgaccag ttactgccgg aaaacgttga gtttatccac
    49081 ggtccggggt gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt
    49141 gccagccatc cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa
    49201 cagggttcgc tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg
    49261 atggatgcgt tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta
    49321 ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat
    49381 gtgcagaatt tttacttctt ctgccagcat attacgctta tcccgacgct tcgcagtttg
    49441 ctggaacagc cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt
    49501 atcggcactg atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtggct
    49561 ggtttcgaac cgcttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg
    49621 gcccacagca aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg
    49681 gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta
    49741 ggcgtgattg aatcttctgg tgtacacctg acgccagatt atcaacgatt cgatgccgaa
    49801 gcgcatttcc gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcacg ttgtggtgaa
    49861 gtattaacgg gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct
    49921 caaaccgcat ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag
    49981 tatcgtcagc aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc
    50041 aggcgatgca gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg
    50101 cagaacagga agatcaggca cgtcttgagc tggcgcagct ggtagcggaa ggcgaccgtc
    50161 tggcgttctc caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg
    50221 gcaagctggc gatttgcggc actgcgaatg acgttgcggt cagtggcgct attccgcgct
    50281 atctctcctg tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag
    50341 tgaccagcat ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta
    50401 aagtggtgca gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa
    50461 ttccggcgaa tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttactggtta
    50521 gcggcacgct cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg
    50581 atggcgaact ggtcagcgac tgcgcggtac tgacgccgct tattcagacg ctgcgtgaca
    50641 ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg tgtgaacgcg gtagttcatg
    50701 agttcgcggc agcctgcggt tgtggtattg aaatttcaga agcggcactg ccggttaaac
    50761 ctgccgtgcg cggcgtttgc gaactgctgg gactggacgc ccttaacttt gccaacgaag
    50821 gcaaactggt gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt
    50881 cccatccact gggaaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc
    50941 gtcttgccgg tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc
    51001 cgcgtatatg ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc
    51061 tttgcggtgc tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag
    51121 caagggttgt tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt
    51181 gaggctcttt cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg
    51241 tggcaagcgc agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga cacccccatt
    51301 aaatatgaag acgaaactgt tctggcacac ggtccggtac gccgcatttt gtcgcgccct
    51361 gatacgctgc attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggtcgcaggt
    51421 gggctatacc ccaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt
    51481 tttggtggct gtgaatttat tcgttatgac gatcgtccct ggagcgaaaa agagttcaat
    51541 cgtctgcaaa catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc
    51601 gtcaacaatg tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt
    51661 gccattacca acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc
    51721 aaagaaatcc attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt
    51781 aaaaacaaac tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa
    51841 cagagcgaag tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg
    51901 ctgctgatca atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcgac
    51961 acctggggca accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg
    52021 ctgggcgtgc tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat
    52081 ttgctgcgcc agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag
    52141 gaaatccatc gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc
    52201 aacaatgttg atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg
    52261 cttaaacaag ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact
    52321 ggcacgggta aagagctgat tgcccgcgcg atccataatc tcagtgggcg taataatcgc
    52381 cgcatggtca aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt
    52441 ggtcatgagc gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg
    52501 gcggataaaa gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg
    52561 aagttgctgc gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt
    52621 cagacggacg tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac
    52681 cgtgagttcc gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca
    52741 ctacgcgagc gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc
    52801 cgtcgtctgg ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac
    52861 atggagtggc cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta
    52921 acacgcggca acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg
    52981 ccgcctgccg caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc
    53041 gtgctgaaag aaaccaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg
    53101 ctgaaacgca cgaccctgct gtcacggatg aagcggcttg gaattgataa atcggcattg
    53161 atttaactgc aaattgccgg acagatctgc ctgtccggca tactattcac gagttttttt
    53221 cggacgatat ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac
    53281 ggttaagatc ggatgacgcc acagcattcg cggcccggcc caacgcataa tctgtttcat
    53341 ctcttcacgc tttgctggct ggtaacagtg caccggacac tgcttacagg ctggtttctc
    53401 ttcgccgaac acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg
    53461 ctccggctcc gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat
    53521 cgtcagtttt tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgt
    53581 atttatatta catcttaatc ttaaagggca ctatgactct aaagaagaaa ggttcgccaa
    53641 ccgacgcaat tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aacagcatct
    53701 ttcccggaac cagcatcaag aactcaccgt tcgcttcttc ccctgaaatg attaactccg
    53761 gtatcatgtg cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata
    53821 tcagggaacc ggacataacc ccatgagtgc aatagaaaat ttcgacgccc atacgcccat
    53881 gatgcagcag tatctcaggc tgaaagccca gcatcccgag atcctgctgt tttaccggat
    53941 gggtgatttt tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat
    54001 ttcactgacc aaacgcggtg cttcggcggg agagccgatc ccgatggcgg ggattcccta
    54061 ccatgcggtg gaaaactatc tcgccaaact ggtgaatcag ggagagtccg ttgccatctg
    54121 cgaacaaatt ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat
    54181 cgttacgcca ggcaccatca gcgatgaagc cctgttacag gagcgtcagg acaacctgct
    54241 ggcggctatc tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagctccgg
    54301 gcgttttcgc ctgagcgaac cggccgaccg cgaaacgatg gcggcagagc tgcaacgcac
    54361 taatccggcg gaactgctgt atgcagaaga tttcgccgag atgtcgctga ttgaaggccg
    54421 tcgcggcctg cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt
    54481 gaatctgcaa tttggcactc gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg
    54541 actttgtgct gccggttgtc tgttgcagta tgcgaaagat acccaacgta cgactctgcc
    54601 gcatattcgt tccatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac
    54661 gcgtcgtaat ctggaaatca cccagaacct ggcgggtggt gcggaaaata cgctggcttc
    54721 tgtgctcgac tgcaccgtca cgccgatggg cagccgtatg ctgaaacgct ggctgcatat
    54781 gccagtgcgc gatacccgcg tgttgcttga gcgccagcaa actatcggcg cattgcagga
    54841 tttcaccgcc gagttgcagc cggtactgcg tcaggtcggc gacctggaac gtattctggc
    54901 acgtctggct ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca
    54961 gcaactgccg gagctgcgtg cacagttaga aactgtcgat agtgcaccgg tacaggcgct
    55021 acgtgagaag atgggcgagt ttgccgagct gcgcgatctg ctggagcgag caatcatcga
    55081 cacaccgcca gtgctggtac gcgacggtgg tgttatcgca tcgggctata acgaagagct
    55141 ggatgagtgg cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg
    55201 cgagcgtgaa cgtaccggcc tggacacgct aaaagttggc tttaatgcgg tgcacggcta
    55261 ctacattcaa atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca
    55321 gacgctgaaa aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt
    55381 tctcacctca aaaggcaaag cactggctct ggaaaaacag ctttatgaag aactgttcga
    55441 cctgctgttg ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga
    55501 cgtgctggtg aacctggcgg aacgggccta taccctgaac tacacctgcc cgacctttat
    55561 tgataaaccg ggcattcgca ttaccgaagg ccgccatccg gtggttgaac aggtgctgaa
    55621 tgagccgttt atcgccaacc cgctgaatct gtcgccgcag cgtcgcatgt tgatcatcac
    55681 cggtccgaac atgggcggta aaagtaccta tatgcgccag accgcgttga ttgcgctaat
    55741 ggcctatatc ggcagctacg taccggcgca aaaagtcgag attggcccga ttgaccgtat
    55801 ctttacccgc gtaggcgcgg cagatgacct ggcttccggg cgttcgacct ttatggtgga
    55861 gatgaccgaa accgccaata ttttacataa tgccaccgaa tatagtctgg tattgatgga
    55921 tgagatcggg cgtggaacgt ccacttacga tggtctgtcg ctggcgtggg cgtgcgcgga
    55981 aaatctggcg aataagatta aggcattgac gctgtttgcc acccactatt tcgagctgac
    56041 acagttaccg gagaaaatgg aaggcgtcgc caacgtgcat ctcgatgcac tggagcacgg
    56101 cgacaccatt gcctttatgc acagcgtgca ggatggcgcg gcgagcaaaa gctacggcct
    56161 ggcggttgca gctctggccg gcgtgccaaa agaggttatt aagcgcgcac ggcaaaagct
    56221 gcgtgagctg gaaagcattt cgccgaacgc cgccgctacg caagtagatg gtacgcaaat
    56281 gtctttgctg tccgtaccag aagaaacttc gcctgcggtc gaggcactgg aaaacctgga
    56341 cccggattca ctgactccgc gtcaggcgct ggagtggatt tatcgcttga agagtctggt
    56401 gtaataaaaa ttcccgatag tcttttgcta tcgggaatat taacgacaac tgacgaatca
    56461 aataaaaata ccctgtataa taggaaagct tattttacag ggtaaaacca tgccatctac
    56521 acgctatcaa aaaatcaatg cccatcacta tcgccatata tgggtcgttg gtgatattca
    56581 tggtgaatat cagttattac aatcccgctt acatcaactc tcttttttcc ccgaaaccga
    56641 cttacttatt tctgtcggcg ataatattga tcgtgggccg gagagtcttg acgtcctgcg
    56701 cctgctaaac cagccctggt ttatctccgt taaaggcaac cacgaagcaa tggcgctgga
    56761 tgcgttcgcg accggcgatg gcaatatgtg gcttgccagc ggtggtgact ggtttttcga
    56821 tttaaatgat tcagagcaaa aagaagctac agatctgttg ctgaaattcc atcaccttcc
    56881 acatattatt gaaatcacta acgacaacat aaaatatgtc atcgcacatg cagattatcc
    56941 ggggagtgaa tatctctttg gtaaagaaat agcggagagc gaattactct ggcctgttga
    57001 tcgtgtgcag aaatcgctta atggcgagtt acaacaaata aacggcgctg attattttat
    57061 atttggacat atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac
    57121 cggatcgccg aaaagtgggc ggctgtcatt ttataaaata aggtagtctc atgcttcttc
    57181 tgtgaagcat gagtaaccag atgttattgc aggccattat tcatttttcg ctaccagcaa
    57241 agagagatcc tgcttcgcca gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtaat
    57301 aatctgatca aactcgctta atggtaacgc cagccatgtc gccacctgac catatttcgt
    57361 cgcatcacag accaaaactc gctggcggct ggcactggca atcgcccgtt tcaccgtgac
    57421 tttatcttcc gctggcgtag aaatcccccg cacactccat gacgatgcag aaataaaagc
    57481 ctgatcaatc atcaggctgc gcagcatggt cgcagcggct tccccgacac aggaacggtt
    57541 ttcccgacac actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta
    57601 gttcgcaata acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg
    57661 tgctatcgct aacgtggtcg ttcccgcatc cagatagata caacttcccg gctgaacaag
    57721 acttgccgcc agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag tttttacctg
    57781 atgagaaggt tcatgcgcca cgcgtcccgg agactggacg cctccggaca ccagcacaac
    57841 ggctccctgc tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg acacattcat
    57901 tctgtccgtt agttcagcaa tactgacaat gcctttttca gctaccatct caaggatgat
    57961 ttggcgacgc tctacgggta tcaacttttg ctccttcctt tgtcctgctg acattctacg
    58021 ctatttgcct gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa aataacacac
    58081 aatgttaatt tatgtgaatc agctcaccat atcgttatct tccagcgctt atattcacaa
    58141 tatcaaacaa aatatcactt aaattaacaa ggagagcaga tgaaaacggg atctgagttt
    58201 catgtcggta tcgttggctt agggtcaatg ggaatgggag cagcactgtc atgtgtccgc
    58261 gcaggtcttt ctacctgggg cgcagacctg aacagcaatg cctgcgctac gttgaaagag
    58321 gcaggtgctt gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg
    58381 ctggtgctgg tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt
    58441 gcacaacatc tgaaacccgg tacggcagta atggtttctt ccactatcgc tagtgctgat
    58501 gcgcaagaaa ttgctaccgc tctggctgga ttcgatctgg aaatgctgga tgcgccagtt
    58561 tctggtggtg cagtaaaagc cgctaacggt gaaatgactg tcatggcctc cggtagcgat
    58621 attgcctttg aacgactggc acccgtgctg gaagccgttg ccggaaaagt ttatcgcata
    58681 ggtgcagaac cgggactagg ttcgaccgta aaaattattc accagttgtt agcgggcgta
    58741 catattgctg ccggagccga agcgatggca cttgcagccc gtgcggggat cccgctggat
    58801 gtgatgtatg acgtcgtgac caatgccgcc ggaaattcct ggatgttcga aaaccggatg
    58861 cgtcatgtgg tggatggcga ttacaccccg cattcagccg tcgatatttt tgttaaggat
    58921 cttggtctgg ttgccgatac agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca
    58981 gcattgaata tgttcaccag cgccagtaac gcaggttacg ggaaagaaga cgatagcgca
    59041 gttatcaaga ttttctctgg catcactcta ccgggagcga aatcatgatc aagattggcg
    59101 ttatcgccga tgattttacc ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc
    59161 taccaacggt acaaattaac ggtgttccaa caggtaaaat gccggaagca atcgacgcac
    59221 tggtgatcag cctgaaaacg cgctcctgtc cggtggttga agccacacag caatcgctgg
    59281 cggctctgag ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa tactgctcta
    59341 ctttcgacag tacggcgaaa ggtaatatcg gcccggttac cgatgcatta atggatgctc
    59401 tcgacacgcc gtttacggtc ttctctccgg ccctgccggt caacggacgt acggtttatc
    59461 aggggtattt gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc catcacccgg
    59521 taaatcccat gaccgacagc tatcttcccc gtctggttga agcgcaatcc acagggcgct
    59581 gcggcgtcgt ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt caagagctgg
    59641 ctcgcttaca gcaagagggc taccgctacg cggtgcttga tgcgctgacc gaacaccatc
    59701 tggaaattca gggagaagcc ttgcgcgatg ccccactggt aacgggcggt tctggtctgg
    59761 cgattggcct ggcccggcag tggacgcaag aaaacggtaa ccaggctcgc aaagcagggc
    59821 atccgctcgc tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg accaaccgcc
    59881 aggtagcaca ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca cgctgcctct
    59941 caactgaaac tctggctact tatgcacacg aactggcaga gtgggttctg ggccaggaaa
    60001 gtgtacttgc tccactggtt tttgccaccg ccagcactga cgcattggca gcaattcaac
    60061 agcaatacgg tgcacaaaaa gccagtcagg cagtagaaac actgttttct caactagcgg
    60121 cgcggttagc ggcggaaggc gtggcacgct ttattgtcgc aggcggtgag acctccggcg
    60181 tagtcacaca gagcctgggc ataaaagggt ttcatattgg cccaaccatt tcccccggcg
    60241 tgccgtgggt aaacgcactg gataagcctg tctcactcgc ccttaaatct ggcaacttcg
    60301 gtgatgaagc ctttttttca cgagcccaaa gagagttttt atcatgagcg atttcgcaaa
    60361 agtagagcag tctttgcgag aggagatgac gcggattgcc agttcattct ttcagcgcgg
    60421 ctatgcaacc ggttcggctg gcaatctgtc gctgctttta cctgacggga atttactggc
    60481 gacaccgaca ggttcatgcc tgggcaatct cgatccgcag cggctttcca aagtcgccgc
    60541 ggatggcgaa tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc atctggcgct
    60601 gtatcgcaac aatccgcgct gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc
    60661 gctttcctgc ctgcaagggc tggacagcag caacgttatt cgtccgttca caccatacgt
    60721 ggtgatgcgg atgggaaatg tcccgctggt gccttattac cgaccgggcg ataaacgcat
    60781 cgcacaggat ctggcggaac tggcagcaga caatcaggct tttttactgg caaatcatgg
    60841 cccagtggtt tgcggtgaaa gcctgcaaga agccgccaac aatatggaag agctggagga
    60901 aacggcaaag ctgattttta ttctcggtga ccgcccgatc cgttatctga ccgcaggtga
    60961 aattgcggaa ttaaggagtt aatgcaatgc ctcgttttgc agctaattta tccatgatgt
    61021 tcaccgaagt gccttttatt gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg
    61081 tggaatttct gtttccctat gactactcca ccctgcaaat ccaaaagcaa ctggagcaaa
    61141 accatctgac actggcgctg ttcaataccg ctcccggaga tattaatgcg ggggaatggg
    61201 gattatccgc ccttcccgga cgtgagcacg aagcacacgc agacatcgac ctggcactgg
    61261 aatatgcatt ggcgctcaat tgtgaacaag tccatgtgat ggcaggcgtc gtgcccgctg
    61321 gcgaagacgc tgaacggtac cgggcagtat ttatcgataa tctccgctac gctgccgacc
    61381 gctttgcgcc acacggcaag cgaatattag ttgaagcact cagccccggc gttaagcctc
    61441 attatctctt ctccagccag tatcaggcac tggctattgt tgaagaggtt gcgcgagata
    61501 acgtgtttat tcaactagat acttttcatg cgcaaaaagt agatggcaac ctgacgcatt
    61561 taatccgcga ctacgctggg aaatatgcgc acgtacaaat tgccggacta cctgatcgac
    61621 atgaaccgga cgatggagaa atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg
    61681 gatatcaggg ctggatcggt tgtgaatata aacctcgtgg cctcaccgaa gaagggcttg
    61741 gctggtttga cgcctggcgc taattcgtta aatccctaat tacaacgtac ccatacatcc
    61801 ccccatctac ggatggggta ggttaactat aactttcaga cagggtttcc catgtccaca
    61861 attacattgt tatgcattgc gttagctggc gtaatcatgc tgttgctgct ggtcatcaag
    61921 gcaaaggtac aaccattcgt tgctctgctc ctcgtcagcc tgttagtcgc acttgcggcg
    61981 ggtataccgg cgggcgaagt gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt
    62041 tccgtcacta ttattattgg tctgggcgct atgctggggc gtatgatcga acactctggt
    62101 ggtgcagagt cactggctaa ttatttcagt cgcaagttag gtgacaaacg aactatcgct
    62161 gcgctgactc tggcagcgtt cttcctcggt attcccgtct tctttgatgt cggctttatt
    62221 attcttgcgc caatcattta cggttttgcc aaggttgcca aaatatcgcc actcaaattt
    62281 ggcctgcctg tcgctggaat catgctaact gttcatgtgg cggtaccgcc gcatccaggc
    62341 cctgtcgccg cagcggggtt actccacgca gacatcggct ggctaaccat catcggtatt
    62401 gcgatttcta ttcccgtagg gattgttggc tactttgcag cgaaaataat caatacgcgc
    62461 caatatgcga tgtcggtgga agtactggaa cagatgcaac tggctccggc cagtgaggaa
    62521 ggcgcaacaa aattaagcga taaaataaat ccaccgggcg tcgcgctggt cacctcgcta
    62581 attgttattc ctatcgcgat tatcatggcg ggtacggttt ccgcaacact gatgccgcct
    62641 tcgcatcccc tgcttggtac gctacagctg atcggctcac caatggtagc tctaatgatt
    62701 gcgctggtgc tggcattctg gttattggct ttacgtcgcg gctggagctt acaacacacc
    62761 agcgacatta tgggctcagc ccttcccact gcggcggtag tgattttggt tactggtgct
    62821 ggaggggtat ttggcaaagt gctggtggaa tcgggcgtcg gcaaagccct tgccaatatg
    62881 ctacaaatga ttgacctgcc tctgttacca gccgcattta ttatttcact ggctctgcgt
    62941 gcatcgcagg ggtcagcaac cgtagcaatc ctgacaaccg gcgggttact ctcagaagcg
    63001 gtgatgggat tgaatccgat tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg
    63061 cttggcgcgt cacatattaa tgactcaggg ttctggattg tgaccaaata tctggggttg
    63121 tcggtagcag acggtctgaa aacctggaca gtgttaacga ccattctcgg ttttaccgga
    63181 ttcttaatta cctggtgcgt atgggcggta atttgacctt aataaaaagg tccgatgggc
    63241 atcggacctt ttatttaacg cttaccttcc accagcagcg gcgggatgct cggcaccatt
    63301 ggcgcgtcat caatatcttt ctgcgtcatg cggaacgctt cgggataatg ttcgcggctg
    63361 gtacggcgca gcggttcggt atcgcgcagc ggttcggtat cgcgccaggt ataaaggcaa
    63421 tgctggcact gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc
    63481 tgttcatcgg cacaacgtgg acaaatcatc ttcttctcct tatttacgtg cagccagcat
    63541 agcggtcagt ttttcagccc aggctttggt ttccggtaaa tccaccaccg gttggctgta
    63601 gtgaccacgg ttgtccgggg cgacaggcgt agtggcgtcg ataatcagct tgtcggtgat
    63661 ccccgcaggg cttgagcctg gatcgagttc cagcacggac atattcggca actgcaccaa
    63721 atcccctgcc gggttcactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg
    63781 gtcaacgtct tcatcgacca taatcaccat cttcacgtag cccagaccat gcggcgtggt
    63841 cattgcgcgc aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat
    63901 cgccagcagg ccatgggtgt acatggcgtt taccgcctgc acttcaggga actcggcttt
    63961 cagctgctga tacagcggca cgcaggtggc tggccccatc aggtagtcga tttcggtcca
    64021 cggcataccg aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat
    64081 gcgcaccacg gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc
    64141 ttcgatttcg cgtttacggc tttcgatgac cccttcgagg atcacttctg aaccccacgg
    64201 cacatcaaaa ccggtcaacg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc
    64261 tgccatttcg tactcggact gatcatattt cagcggcgtg gcccccatca gcgtgatgat
    64321 cggatcgtta ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt ctgctttatg
    64381 cagatgcagg gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt
    64441 cacttccatg cggtagatgc cgacgttctg cttgccgaag ttatccgggt cgagcggatc
    64501 gcgggaaacc acgcacgctt tgtcgagata gaaaccgcca tcgccatcgt ttaaacgaaa
    64561 cagcggcagg atatcgaaca ggttaatctc ttcgccatcg acggtgttct gcgcccaggc
    64621 tggattggcg cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc
    64681 aatctgcttt ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca
    64741 ggaaccgatg gtattcatcg tcacgcgggc atcggtaaag ccgcgaatat tatcaaacca
    64801 cagcgcgggc gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg
    64861 ctcagcgttc acctcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa
    64921 aaagctgcgt aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc
    64981 ggcaatcctt gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg
    65041 gctaccacat ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc
    65101 ggcggcacca tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg
    65161 ctaagcggca tttctcgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc
    65221 gccgcgcgcc ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata
    65281 ctgcacggaa taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc
    65341 tgatccgccg ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc
    65401 gtttccagtt caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc
    65461 ggcatctccc gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc
    65521 atcccgacga tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt
    65581 aacatactcc ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca
    65641 ctaaaaaacc gctatgatag caacagagaa ttagcccgga gtttacatgg cgttacgaaa
    65701 taaagcgttc catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga
    65761 gttacctgac ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg
    65821 tatcgaacag gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat
    65881 gttggcaaga atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg
    65941 gcgacgcttt gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat
    66001 tggtgacagc gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt
    66061 tatgcagctg gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta
    66121 tcgcgtctta tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc
    66181 tcgcttgaga ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat
    66241 tcagcccctg cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc
    66301 gaacacgttc acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt
    66361 accccagcaa accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca
    66421 gccatttgac gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct
    66481 ctttttcatc ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt
    66541 ctaccgaggt aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat
    66601 ccagttgctc tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac
    66661 gtgcggttcg caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac
    66721 gggtttggtt cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg
    66781 agaagcggaa accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc
    66841 cctcttcgat aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta
    66901 ccaccagacg caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg
    66961 cgcgacgcgc aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac
    67021 caaggtaaag ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct
    67081 cttcggccaa atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct
    67141 caactccgtt ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct
    67201 gactcataag gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcatggt
    67261 tccgccgatt tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg
    67321 aatttcaaaa tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg
    67381 ccccgccttc acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact
    67441 caggtaatca tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc
    67501 agcataaaca acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat
    67561 atcaatcccc ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt
    67621 cggccagcgc caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt
    67681 tgtgcttgct gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt
    67741 cggcaacatt ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg
    67801 cgacgcaaca gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc
    67861 gtcggcctgg gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg
    67921 caaggtctga ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc
    67981 acggaaatcg ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt
    68041 ataggtactg ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat
    68101 gcgtccgttt tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg
    68161 ttgttgagta gcctgaattt gtggctgctg taccggctga atttgcggtt gctgggctgt
    68221 agacgtcgtc cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg
    68281 cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc
    68341 cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc
    68401 cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa
    68461 ggcaccctac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc
    68521 aggaaaaatc caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa
    68581 agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca
    68641 aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca
    68701 ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc
    68761 gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat
    68821 ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat
    68881 gctggacaag atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca
    68941 cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg
    69001 aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg
    69061 cttcatcaac gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct
    69121 gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt
    69181 gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa
    69241 atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc
    69301 cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg
    69361 atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat
    69421 acctttgatt tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg
    69481 caacggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta
    69541 atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat
    69601 cgcggcggct accgtaccgg aataaataac gtcatccccc agattcggcc cggcgttaat
    69661 tccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca
    69721 atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag
    69781 ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac
    69841 ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac
    69901 cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga
    69961 caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat
    70021 cccaccaatt ccagcttaat tgttgcggat acagcaacat cgctctgcgc gcggcttcaa
    70081 ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gccttttcga
    70141 atgccagcgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca
    70201 tcagctcttt atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgctacaa
    70261 accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg
    70321 cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc
    70381 aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc
    70441 cctgcaagtt gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt
    70501 ttacgcaaat atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa
    70561 ccagggtaaa ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat
    70621 actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg
    70681 gcacgcgagc gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc
    70741 tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat
    70801 tgcagccgtt tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc
    70861 ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg
    70921 gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag
    70981 cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt
    71041 agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg
    71101 catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt
    71161 gcccagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct
    71221 gtcggcacct ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc
    71281 cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc
    71341 cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc
    71401 aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt
    71461 cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc
    71521 gcacggcctt cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg
    71581 gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg
    71641 aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca
    71701 tttttgcccg gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc
    71761 gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga
    71821 cgagcggcgt catgcaccaa tacccactgc gcgtcgccag cggctttcaa acctgccagc
    71881 acggaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga
    71941 ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg
    72001 ggatgcgcca gcaacgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga
    72061 tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg
    72121 caaacatcca aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc
    72181 cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc
    72241 ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga
    72301 tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc
    72361 tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac
    72421 agcgaatact gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg
    72481 ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg
    72541 gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg
    72601 gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga
    72661 aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac
    72721 cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc
    72781 caccactacc gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa
    72841 cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa
    72901 accgaccacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt
    72961 atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg
    73021 ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    73081 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    73141 caggcgcttc gtaaacagaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt
    73201 tcttatataa gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca
    73261 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    73321 gcgagataaa tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac
    73381 cgacgcgacg gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc
    73441 cgtggcgaac attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct
    73501 cctccagcac cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca
    73561 ctacaccacg atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc
    73621 agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca
    73681 gtgcggaaag tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga
    73741 tggcgcagca gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt
    73801 gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa
    73861 caccagcggc tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc
    73921 aacttcacgc tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc
    73981 gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa gcggctgttc
    74041 cgccatccat accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc
    74101 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    74161 tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc
    74221 cacaccagaa ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcgt
    74281 tccggcgtaa ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat
    74341 tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg
    74401 accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt ccagcgcaga
    74461 gagcggcaca aagcgaatat ccagattgcc aggtaactgt tcggcgaaag tcaggtagtc
    74521 tttacgaatg cgggtgaatg tctcttcact gtaatccacc agatccattt tgttgatcgc
    74581 tacgaccaga tgtttgatcc ccagcagcgt ggagataaaa ctgtgacgac gggtttgatc
    74641 gagcacgcct ttacgggcat cgatcagtaa gatcgccagc tcacacgttg aggcgccggt
    74701 cgccatattg cgggtatact gctcgtgccc gggggtgtcg gcgataataa atttacgctt
    74761 ctcggtagag aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg
    74821 caggccgtcc accagcagag ccagatccag tttttcgccc tgggtgccgt gacgcttact
    74881 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg
    74941 accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct
    75001 tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc
    75061 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct
    75121 ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga
    75181 tgatttccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca
    75241 gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    75301 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat
    75361 atagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    75421 agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt
    75481 gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt
    75541 aaattcgctc tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat
    75601 caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt
    75661 tcgcgctgcc gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    75721 ccagcagttc gcagccgtag gctttagcag tacgatcgcg gaactcatac atctcgcgga
    75781 atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct
    75841 tgcgcgccaa atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    75901 ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    75961 ggtgagtaag tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca
    76021 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    76081 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    76141 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg
    76201 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    76261 accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa
    76321 cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg
    76381 accggaactc cagcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    76441 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    76501 cgcaagaact ggcataacgt gacaggaagt acggtgattg ccgctcatga aggcaaagcg
    76561 ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac
    76621 gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta
    76681 ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    76741 tttgtggcga ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg
    76801 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    76861 ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg
    76921 cgcgacaggg cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggt
    76981 ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa
    77041 gcgggcattg ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt
    77101 tatcagcaac gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga
    77161 ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt
    77221 gatgttatgc ggataatgct accgctggtg aaggagctgg cgaaggcgtc ttgatgggtt
    77281 tgaaaatggg agctgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat
    77341 cgcatccagt gcgcccggtt tatccccgct gacgcgggga acacaaaacc aaacttctcc
    77401 ataaattcca tagccgcggt ttatccccgc tggcgcgggg aacacgagtc tatcagcgac
    77461 actaccggca atagcgacgg tttatccccg ctggcgcggg gaacacggct ttaaaattgt
    77521 gttcccgtct ggcgctgggg gtttatcccc gctggcgcgg ggaacacgca tccatgccga
    77581 cgcctttacg tgtgcggggc ggtttatccc cgctggcgcg gggaacacgc gcgaatttgt
    77641 gcgcatgggg cgcatttttg gcggtttatc cccgctggcg cggggaacac acgatggcga
    77701 tgcgtgagaa agggggtcga tacggtttat ccccgctggc gcggggaaca cagttcgctg
    77761 agtagccttt tttctgtgcc taacggttta tccccgctgg cgcggggaac acgaggtggc
    77821 aatacgcgta gatcatttgg tcgtcggttt atccccgctg gcgcggggaa caccaaaata
    77881 ttacgagctt cgtcaggcca tggaccggtt tatccccgct ggcgcgggga acacactaag
    77941 catacataac tgtttttaaa caaatttatt ccgcatcaac aatctaccaa ctaaattaaa
    78001 acatttcctt atttttaaag aacggataac ttattgatta tcaacaggaa gaaaagaaac
    78061 caaacgtaac ccatccaaat ccaccggaat acgtctgttt tctccccagg tctgaaattc
    78121 aaaacccgac tcggtattgg tcgcccaggc catcaccaca tttccgcaac cagccagttg
    78181 ggtaatttgc tgccagatca tctcccgaat acgttttgat gtatcaccaa catacacacc
    78241 ggcacgcact tccagtagcc agattgcgag ccgtccacgt aagcgcggcg ggacattttc
    78301 tgtaacaacc acgaccatgc tcatcagccg cgtcctcgat gaccgctatc gcccaacgtt
    78361 tcaggttcgg ggatggctgg cggtaacata tccggcgcag gttgtggcgg ttcaatttca
    78421 cctgcggcaa ggacttcctc aattaacggt attaatttgc ccgttaactt agtgctacgg
    78481 aaaatatcgc gacaggctaa tctgacttct ttatcaggtt ctgcgggttg cctcgctgct
    78541 atttcaaatg cctttggcac aaccgaatca aatttaatga tatcggctat gtcataaaca
    78601 aatgaaagcg gtttgccact atggataaac ccaatagcgg gcgcatatcc cgcggctaat
    78661 actgccgctt cagatatacc gtacagacat gatgtagcag cactgatgca gcgattcaca
    78721 acatcgcctt tttcccagtc tttaggatcg tatttgcgac cattccattt cacaccatat
    78781 tgtttcgcca gtaatgcata cgtctggcga acgcgagatc cctcaattcc acgtaactgc
    78841 tccactgaac ggcgagctgg ctgtggttca cgaaaacgta attcatacat tttgcgcacc
    78901 accttcaacc gtagatcttc cgttaaagcc agctttgcct ggtagagtaa tttatctgcc
    78961 cgcgccccac cgggttgtcc ggaggagtaa acgcgaacgc ccgcttcacc gacccagacc
    79021 agcagtgttc ccaccgtggc ggccagatgc accgccgcgt gggaaactct cgttcctggt
    79081 tcgagcataa tgcaggcgac cgatcccacc ggaatatgcg ttcggatccc tgttttgtcg
    79141 atcagcacga aagcgccgtc cagtacgtcg atctgaccgt actggaggaa gatcatggag
    79201 gtgcgatctt ttaatgggat cgggctcagt ggtacaaacg tcacgcctct gctccgggtt
    79261 tgatcagcat cagaccacag ccgaacgccc ggcttttacc atacccctgg ctcaggcgtt
    79321 gcacaaataa cccaggatcg gtgaccgtaa gcatccccgt ataatccacg ctactgaact
    79381 ggatcagttg ccgggagttt tcccgccgca gttgctgttg tcggtaggca tcaaccgagg
    79441 tatccagcag tgtaaaaccg cttctctccc cctgcgctgc cagccagtcc agcgccgcct
    79501 gttgttgatg caaccagaca tcacttcctt ccgcctgccc cctcacctgc cgtttcgcct
    79561 ccatcagcag atcgtggcgc ttgcccgctt tacagattgt tggattcgcc cgcaggttga
    79621 aacaaagttg ttgtccggta cacagctcgg gcgcaaatga ccggcattct atggtgaacg
    79681 tatcactttc cgccgggcgt tcctgcgaca gtacaaaaaa gcgaaacgcg ccctggagtt
    79741 cttcccgacg ataaagaaat tgcctttctt tgccgccagg gaagagatcc cacagccact
    79801 gatgcatcac atattcaccg cgatccacca gatgcagcaa ctgcgcaggc gaaagctggc
    79861 cggtatgtaa ggttattctt gagaggtaca tggttcctcc ttgctgagcc acggcccctg
    79921 attgacggtg cgttccccaa acagccactg ctgacgattt aaaggaacat cccggcggcg
    79981 taatattttg ctcgcgacca ggccgtcgtg tttcccttcc caccagcatt catcctgaag
    80041 tttcgggagt gagactttca gtttatggaa atgatcctga tactgttggt atgcattaca
    80101 taaggcatca cacgcgttgc cttcgagcag taacggcgca agaggtaatg ccagcggatg
    80161 actttttcgc cccagataaa gcggaaaaac tggatggcgt aaaccgtcct gcaactgttc
    80221 gaggctgtaa ggcgcatcgg ctgttgttgc caccgccacc atccaccagg catcggtgta
    80281 gtagtcgcgc cgggagataa tcgcgctcaa aagctcaggg tcgctcaact cttcgcgacg
    80341 gctgaaataa cgtgctttac gcacctcttt tggcatctgg accgtgtgat aatcccgtgc
    80401 ccagcgcggg ttacggctgg cgcaaaccac cagtgaatag tggcgattaa acgcgtttaa
    80461 gcgttcggta tcatcacgcc gaatccctac cccggcagcc agtagcccca gcaatgctga
    80521 gcgcgaagga agttcatggg tatgacgaac ttcacctgga gcatcgaccc cccaggacgc
    80581 catcggccca tgaagctgaa aaatcagata ttggctcata agccgcccct tacgcgcaga
    80641 taaagtccag cacgtccttc atgcttccct gtttgttcat cacgtcaaaa cttgcgcatt
    80701 cggtcttctg ttcgtagacc gtattcatgt tttcgcgaag cgttgtaata cgctgcaccg
    80761 ccacatctaa ctgacgtgtg ccattaatgg gttcatagaa agccgccgcc agagagcgtg
    80821 gttgatcggt gcctttttct gccagcgccc aggaggcgta ggcacggctg gcaaagctgt
    80881 tctgtttgcc ggttggggag actttaagtg cggcttccgt aaaggcacgt atggtctgat
    80941 tagctaacgc ttcgtcaccg ccgaggtttt ctaccagcag atctttatca atgcagatat
    81001 aggtgtagaa cagcgcagaa ccgaatccgg tttcatctac atgaccggca ccggcatctt
    81061 cagaagcctg gcgtaaatca tctacggcgg tgaaaaaatc attttcgaca atcgtttcac
    81121 tgacaccaaa cgcatgcgcg acctggcagg cggcttctac attaaacccg ggttttttcg
    81181 ccagcatacg accaaacata gcgatatcta ccgccatacg gtctttacgt aacaaagcga
    81241 gatctttctc ttttggcgcg cgcttttctt ctgccagttg atgggccagc gcttttacgg
    81301 cgtcaaattc tgccgggctg atatggacga gctgttcagt ttcggcgcta gtgagcggat
    81361 cttttggttt tttgtcgttt ttttgttttt tgtctttttt ggctttccca agataatcgg
    81421 ctatctctac tgcccattcg atggcttttt tatcttcgat tcctttctca atcaggatag
    81481 ttgccgcctc acgcgcaata cgcccgctac gaacaccaat atggcccgcc agcgcctgtt
    81541 caaaaagttc agaagtgcgc caagcacgct tcagactttg cgaggaaacg cgcagacgcg
    81601 ttgctccacc cagaatcaca gttttcggag ag
//
LOCUS       DAZGCS010000004        80216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGCS010000004 DAZGCS010000000
VERSION     DAZGCS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.004"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            102..686
                     /gene="yraH"
                     /locus_tag="ACHK01_000259"
     CDS             102..686
                     /gene="yraH"
                     /locus_tag="ACHK01_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8095266.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            766..1461
                     /gene="yraI"
                     /locus_tag="ACHK01_000260"
     CDS             766..1461
                     /gene="yraI"
                     /locus_tag="ACHK01_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8095267.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1491..4007
                     /gene="yraJ"
                     /locus_tag="ACHK01_000261"
     CDS             1491..4007
                     /gene="yraJ"
                     /locus_tag="ACHK01_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8095268.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALYL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4018..5109
                     /gene="yraK"
                     /locus_tag="ACHK01_000262"
     CDS             4018..5109
                     /gene="yraK"
                     /locus_tag="ACHK01_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8095269.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5152..6012)
                     /gene="rsmI"
                     /locus_tag="ACHK01_000263"
     CDS             complement(5152..6012)
                     /gene="rsmI"
                     /locus_tag="ACHK01_000263"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO8095270.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6076..8064
                     /gene="lpoA"
                     /locus_tag="ACHK01_000264"
     CDS             6076..8064
                     /gene="lpoA"
                     /locus_tag="ACHK01_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO8095271.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTTQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFR
                     LEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQV
                     QGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8022..8417
                     /gene="yraN"
                     /locus_tag="ACHK01_000265"
     CDS             8022..8417
                     /gene="yraN"
                     /locus_tag="ACHK01_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO8095272.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8437..9027
                     /gene="diaA"
                     /locus_tag="ACHK01_000266"
     CDS             8437..9027
                     /gene="diaA"
                     /locus_tag="ACHK01_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO8095273.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9037..9612
                     /gene="dolP"
                     /locus_tag="ACHK01_000267"
     CDS             9037..9612
                     /gene="dolP"
                     /locus_tag="ACHK01_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8095274.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9726..10766)
                     /gene="yraQ"
                     /locus_tag="ACHK01_000268"
     CDS             complement(9726..10766)
                     /gene="yraQ"
                     /locus_tag="ACHK01_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8095275.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10839..11474)
                     /gene="yraR"
                     /locus_tag="ACHK01_000269"
     CDS             complement(10839..11474)
                     /gene="yraR"
                     /locus_tag="ACHK01_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO8095276.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11602..12120
                     /gene="yhbO"
                     /locus_tag="ACHK01_000270"
     CDS             11602..12120
                     /gene="yhbO"
                     /locus_tag="ACHK01_000270"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO8095277.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12100..12543)
                     /gene="yhbP"
                     /locus_tag="ACHK01_000271"
     CDS             complement(12100..12543)
                     /gene="yhbP"
                     /locus_tag="ACHK01_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO8095278.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12594..12896
                     /gene="yhbQ"
                     /locus_tag="ACHK01_000272"
     CDS             12594..12896
                     /gene="yhbQ"
                     /locus_tag="ACHK01_000272"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO8095279.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(12883..13386)
                     /gene="yhbS"
                     /locus_tag="ACHK01_000273"
     CDS             complement(12883..13386)
                     /gene="yhbS"
                     /locus_tag="ACHK01_000273"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8095280.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13380..13904)
                     /gene="ubiT"
                     /locus_tag="ACHK01_000274"
     CDS             complement(13380..13904)
                     /gene="ubiT"
                     /locus_tag="ACHK01_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO8095281.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14113..15108
                     /gene="ubiU"
                     /locus_tag="ACHK01_000275"
     CDS             14113..15108
                     /gene="ubiU"
                     /locus_tag="ACHK01_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO8095282.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15117..15995
                     /gene="ubiV"
                     /locus_tag="ACHK01_000276"
     CDS             15117..15995
                     /gene="ubiV"
                     /locus_tag="ACHK01_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO8095283.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16201..17208
                     /gene="yhbW"
                     /locus_tag="ACHK01_000277"
     CDS             16201..17208
                     /gene="yhbW"
                     /locus_tag="ACHK01_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO8095284.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17326..18570)
                     /gene="mtr"
                     /locus_tag="ACHK01_000278"
     CDS             complement(17326..18570)
                     /gene="mtr"
                     /locus_tag="ACHK01_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO8095285.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18724..20613)
                     /gene="deaD"
                     /locus_tag="ACHK01_000279"
     CDS             complement(18724..20613)
                     /gene="deaD"
                     /locus_tag="ACHK01_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO8095286.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20606..20686)
                     /gene="yrbN"
                     /locus_tag="ACHK01_000280"
     CDS             complement(20606..20686)
                     /gene="yrbN"
                     /locus_tag="ACHK01_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO8095287.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20793..21677)
                     /gene="nlpI"
                     /locus_tag="ACHK01_000281"
     CDS             complement(20793..21677)
                     /gene="nlpI"
                     /locus_tag="ACHK01_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO8095288.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21786..23921)
                     /gene="pnp"
                     /locus_tag="ACHK01_000282"
     CDS             complement(21786..23921)
                     /gene="pnp"
                     /locus_tag="ACHK01_000282"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO8095289.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24168..24437)
                     /gene="rpsO"
                     /locus_tag="ACHK01_000283"
     CDS             complement(24168..24437)
                     /gene="rpsO"
                     /locus_tag="ACHK01_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO8095290.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24586..25530)
                     /gene="truB"
                     /locus_tag="ACHK01_000284"
     CDS             complement(24586..25530)
                     /gene="truB"
                     /locus_tag="ACHK01_000284"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO8095291.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25530..25931)
                     /gene="rbfA"
                     /locus_tag="ACHK01_000285"
     CDS             complement(25530..25931)
                     /gene="rbfA"
                     /locus_tag="ACHK01_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO8095292.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26095..28767)
                     /gene="infB"
                     /locus_tag="ACHK01_000286"
     CDS             complement(26095..28767)
                     /gene="infB"
                     /locus_tag="ACHK01_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO8095293.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28792..30279)
                     /gene="nusA"
                     /locus_tag="ACHK01_000287"
     CDS             complement(28792..30279)
                     /gene="nusA"
                     /locus_tag="ACHK01_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO8095294.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQALEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30307..30759)
                     /gene="rimP"
                     /locus_tag="ACHK01_000288"
     CDS             complement(30307..30759)
                     /gene="rimP"
                     /locus_tag="ACHK01_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO8095295.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30966..31042)
                     /locus_tag="ACHK01_000289"
     tRNA            complement(30966..31042)
                     /locus_tag="ACHK01_000289"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31006..31008),aa:Met,seq:cat)
     gene            31390..32733
                     /gene="argG"
                     /locus_tag="ACHK01_000290"
     CDS             31390..32733
                     /gene="argG"
                     /locus_tag="ACHK01_000290"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO8095296.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32741..34366)
                     /gene="yhbX"
                     /locus_tag="ACHK01_000291"
     CDS             complement(32741..34366)
                     /gene="yhbX"
                     /locus_tag="ACHK01_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8095297.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34823..34909)
                     /locus_tag="ACHK01_000292"
     tRNA            complement(34823..34909)
                     /locus_tag="ACHK01_000292"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34873..34875),aa:Leu,seq:gag)
     gene            complement(34924..35256)
                     /gene="secG"
                     /locus_tag="ACHK01_000293"
     CDS             complement(34924..35256)
                     /gene="secG"
                     /locus_tag="ACHK01_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO8095298.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35484..36821)
                     /gene="glmM"
                     /locus_tag="ACHK01_000294"
     CDS             complement(35484..36821)
                     /gene="glmM"
                     /locus_tag="ACHK01_000294"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO8095299.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36814..37662)
                     /gene="folP"
                     /locus_tag="ACHK01_000295"
     CDS             complement(36814..37662)
                     /gene="folP"
                     /locus_tag="ACHK01_000295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO8095300.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVVPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37752..39686)
                     /gene="ftsH"
                     /locus_tag="ACHK01_000296"
     CDS             complement(37752..39686)
                     /gene="ftsH"
                     /locus_tag="ACHK01_000296"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO8095301.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39786..40415)
                     /gene="rlmE"
                     /locus_tag="ACHK01_000297"
     CDS             complement(39786..40415)
                     /gene="rlmE"
                     /locus_tag="ACHK01_000297"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO8095302.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40541..40834
                     /gene="yhbY"
                     /locus_tag="ACHK01_000298"
     CDS             40541..40834
                     /gene="yhbY"
                     /locus_tag="ACHK01_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO8095303.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40990..41466)
                     /gene="greA"
                     /locus_tag="ACHK01_000299"
     CDS             complement(40990..41466)
                     /gene="greA"
                     /locus_tag="ACHK01_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO8095304.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41714..43147
                     /gene="dacB"
                     /locus_tag="ACHK01_000300"
     CDS             41714..43147
                     /gene="dacB"
                     /locus_tag="ACHK01_000300"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8095305.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43187..44359)
                     /gene="cgtA"
                     /locus_tag="ACHK01_000301"
     CDS             complement(43187..44359)
                     /gene="cgtA"
                     /locus_tag="ACHK01_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO8095306.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44375..45340)
                     /gene="yhbE"
                     /locus_tag="ACHK01_000302"
                     /pseudo
     CDS             complement(44375..45340)
                     /gene="yhbE"
                     /locus_tag="ACHK01_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(45467..45724)
                     /gene="rpmA"
                     /locus_tag="ACHK01_000303"
     CDS             complement(45467..45724)
                     /gene="rpmA"
                     /locus_tag="ACHK01_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO8095307.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45745..46056)
                     /gene="rplU"
                     /locus_tag="ACHK01_000304"
     CDS             complement(45745..46056)
                     /gene="rplU"
                     /locus_tag="ACHK01_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO8095308.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46315..47286
                     /gene="ispB"
                     /locus_tag="ACHK01_000305"
     CDS             46315..47286
                     /gene="ispB"
                     /locus_tag="ACHK01_000305"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO8095309.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVQEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47514..47792
                     /gene="sfsB"
                     /locus_tag="ACHK01_000306"
     CDS             47514..47792
                     /gene="sfsB"
                     /locus_tag="ACHK01_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO8095310.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47840..49099)
                     /gene="murA"
                     /locus_tag="ACHK01_000307"
     CDS             complement(47840..49099)
                     /gene="murA"
                     /locus_tag="ACHK01_000307"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO8095311.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49154..49408)
                     /gene="ibaG"
                     /locus_tag="ACHK01_000308"
     CDS             complement(49154..49408)
                     /gene="ibaG"
                     /locus_tag="ACHK01_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO8095312.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49568..49861)
                     /gene="mlaB"
                     /locus_tag="ACHK01_000309"
     CDS             complement(49568..49861)
                     /gene="mlaB"
                     /locus_tag="ACHK01_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO8095313.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49861..50496)
                     /gene="mlaC"
                     /locus_tag="ACHK01_000310"
     CDS             complement(49861..50496)
                     /gene="mlaC"
                     /locus_tag="ACHK01_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO8095314.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50515..51066)
                     /gene="mlaD"
                     /locus_tag="ACHK01_000311"
     CDS             complement(50515..51066)
                     /gene="mlaD"
                     /locus_tag="ACHK01_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO8095315.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51071..51853)
                     /gene="mlaE"
                     /locus_tag="ACHK01_000312"
     CDS             complement(51071..51853)
                     /gene="mlaE"
                     /locus_tag="ACHK01_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO8095316.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51861..52670)
                     /gene="mlaF"
                     /locus_tag="ACHK01_000313"
     CDS             complement(51861..52670)
                     /gene="mlaF"
                     /locus_tag="ACHK01_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO8095317.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52880..53857
                     /gene="yrbG"
                     /locus_tag="ACHK01_000314"
     CDS             52880..53857
                     /gene="yrbG"
                     /locus_tag="ACHK01_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO8095318.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53871..54857
                     /gene="kdsD"
                     /locus_tag="ACHK01_000315"
     CDS             53871..54857
                     /gene="kdsD"
                     /locus_tag="ACHK01_000315"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO8095319.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELEQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54878..55444
                     /gene="kdsC"
                     /locus_tag="ACHK01_000316"
     CDS             54878..55444
                     /gene="kdsC"
                     /locus_tag="ACHK01_000316"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO8095320.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55441..56016
                     /gene="lptC"
                     /locus_tag="ACHK01_000317"
     CDS             55441..56016
                     /gene="lptC"
                     /locus_tag="ACHK01_000317"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO8095321.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55985..56542
                     /gene="lptA"
                     /locus_tag="ACHK01_000318"
     CDS             55985..56542
                     /gene="lptA"
                     /locus_tag="ACHK01_000318"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO8095322.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56549..57274
                     /gene="lptB"
                     /locus_tag="ACHK01_000319"
     CDS             56549..57274
                     /gene="lptB"
                     /locus_tag="ACHK01_000319"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO8095323.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57322..58755
                     /gene="rpoN"
                     /locus_tag="ACHK01_000320"
     CDS             57322..58755
                     /gene="rpoN"
                     /locus_tag="ACHK01_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO8095324.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58778..59065
                     /gene="hpf"
                     /locus_tag="ACHK01_000321"
     CDS             58778..59065
                     /gene="hpf"
                     /locus_tag="ACHK01_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO8095325.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59183..59674
                     /gene="ptsN"
                     /locus_tag="ACHK01_000322"
     CDS             59183..59674
                     /gene="ptsN"
                     /locus_tag="ACHK01_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO8095326.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLEAPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59720..60574
                     /gene="rapZ"
                     /locus_tag="ACHK01_000323"
     CDS             59720..60574
                     /gene="rapZ"
                     /locus_tag="ACHK01_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO8095327.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60571..60843
                     /gene="npr"
                     /locus_tag="ACHK01_000324"
     CDS             60571..60843
                     /gene="npr"
                     /locus_tag="ACHK01_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO8095328.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61057..61689
                     /gene="yrbL"
                     /locus_tag="ACHK01_000325"
     CDS             61057..61689
                     /gene="yrbL"
                     /locus_tag="ACHK01_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO8095329.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61686..62414)
                     /gene="mtgA"
                     /locus_tag="ACHK01_000326"
     CDS             complement(61686..62414)
                     /gene="mtgA"
                     /locus_tag="ACHK01_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO8095330.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62411..63064)
                     /gene="elbB"
                     /locus_tag="ACHK01_000327"
     CDS             complement(62411..63064)
                     /gene="elbB"
                     /locus_tag="ACHK01_000327"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO8095331.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63294..65630)
                     /gene="arcB"
                     /locus_tag="ACHK01_000328"
     CDS             complement(63294..65630)
                     /gene="arcB"
                     /locus_tag="ACHK01_000328"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO8095332.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLCFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65726..66655)
                     /gene="yhcC"
                     /locus_tag="ACHK01_000329"
     CDS             complement(65726..66655)
                     /gene="yhcC"
                     /locus_tag="ACHK01_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO8095333.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67330..71790
                     /gene="gltB"
                     /locus_tag="ACHK01_000330"
     CDS             67330..71790
                     /gene="gltB"
                     /locus_tag="ACHK01_000330"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO8095334.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71803..73221
                     /gene="gltD"
                     /locus_tag="ACHK01_000331"
     CDS             71803..73221
                     /gene="gltD"
                     /locus_tag="ACHK01_000331"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO8095335.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEDNMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73405..74532
                     /gene="yhcG"
                     /locus_tag="ACHK01_000332"
     CDS             73405..74532
                     /gene="yhcG"
                     /locus_tag="ACHK01_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO8095336.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74592..75056)
                     /gene="nanQ"
                     /locus_tag="ACHK01_000333"
     CDS             complement(74592..75056)
                     /gene="nanQ"
                     /locus_tag="ACHK01_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO8095337.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(75053..75928)
                     /gene="nanK"
                     /locus_tag="ACHK01_000334"
     CDS             complement(75053..75928)
                     /gene="nanK"
                     /locus_tag="ACHK01_000334"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8095338.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75925..76614)
                     /gene="nanE"
                     /locus_tag="ACHK01_000335"
     CDS             complement(75925..76614)
                     /gene="nanE"
                     /locus_tag="ACHK01_000335"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO8095339.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76662..78152)
                     /gene="nanT"
                     /locus_tag="ACHK01_000336"
     CDS             complement(76662..78152)
                     /gene="nanT"
                     /locus_tag="ACHK01_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO8095340.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78261..79154)
                     /gene="nanA"
                     /locus_tag="ACHK01_000337"
     CDS             complement(78261..79154)
                     /gene="nanA"
                     /locus_tag="ACHK01_000337"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8095341.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79276..80067)
                     /gene="nanR"
                     /locus_tag="ACHK01_000338"
     CDS             complement(79276..80067)
                     /gene="nanR"
                     /locus_tag="ACHK01_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8095342.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19780 a        21660 c        19893 g        18883 t
ORIGIN      
        1 aatgttcaat gaaatatttc tacctgtata atctttaaag atgttgaaca tatattcaca
       61 ttaaatatga ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa
      121 cagctattgt gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa
      181 tagtttttga tggcgagatt ttaaaatccg cttgtgaaat caatgactct gataagaaaa
      241 ttgaagttgc tcttggtcac tataatgccg aacagtttcg ttctgttggc gatcgtagcc
      301 caaaaattcc attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata
      361 acggcaacgt tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggcaccgtcc
      421 ctaacttccc taatctggca aaagtgggct cttttgctgg cacagcggca acaggtgtgg
      481 gtatccgtat tgacgatgcg gaaagcggaa accttatgcc actgaatgct atggggaatg
      541 ataacacggt ttatcagatc ccagcggact ccaatggtat tgtcaatgtt gacctcatcg
      601 cttactacgt atcaaccgtt gaagcatcag aaatcacccc aggggaagca gacgctgtcg
      661 ttaacgtaac gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg
      721 cccttttcct tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca
      781 ttcgcggtga taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt
      841 atcgttttac agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct
      901 gtcgcaaaca aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat
      961 ggtacaagcc gtgccccatt tattataacc ccaccgctat ttcgtcttga agctggcgat
     1021 gactcatcac tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg
     1081 ttctacatta atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac
     1141 gagttgacgc tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag
     1201 ggacgggtaa acgatgcgtg gacatcactg gaatttaaac gtagtgacca ttcactcaat
     1261 atatataacc caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat
     1321 ctcacaagca aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca
     1381 tctggcggaa agaacgtgaa atgggctgca atcaatgatt atggcggcag ttccgggaca
     1441 gaaactcgtc cactgcaata acaaatataa aaaacacagg tcatcaggga atgccacaac
     1501 gacaccacca gggacataaa cgcacaccga aacagttggc gctcatcatc aaacgctgtt
     1561 tgccgatggt gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt
     1621 atttcgaccc cattatgctg gaaactacaa aaagtggtat gcaaacaact gatctgtcac
     1681 gtttttcaaa aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata
     1741 aaaagaaggt ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc
     1801 cacagtttac ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc
     1861 tggctgaaaa agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag
     1921 ctgctgaatt tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt
     1981 accgtgatgc aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt
     2041 ttaccaacta ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac
     2101 gacagtacct gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt
     2161 ctacgtggac acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac
     2221 gtgatatcaa ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta
     2281 ttttttccag ctacaccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa
     2341 acagccagcg cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga
     2401 ctatcaggca aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa
     2461 ttaacgatct ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg
     2521 acggtacgca acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg
     2581 ggcatctaaa atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca
     2641 aggaacccga atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt
     2701 atggcggcct gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac
     2761 ttggcgcact gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc
     2821 agcactcttt tcatggctat caatggcgta cgcagtacat caaagatatc ccggaaacca
     2881 acaccaatat cgctgtcagc tactatcgct ataccaacga tggctatttt agttttaatg
     2941 aagccaatac ccgtaattgg aactataaca gtcgccaaaa aagtgaaatt caattcaaca
     3001 tcagccagac aatatttgat ggggtaagtc tgtatgcctc cggttcgcag caagactatt
     3061 ggggcaataa cgagaaaaac aggaatatct ctgttggggt ttccggtcag caatggggaa
     3121 ttggttacag cctgaattat caatacagtc gctacacgga tcaaaataat gaccgcgcac
     3181 tctatttgaa tctcagtatt ccgttagaac gctggttacc gcgtagccgg gtttcctatc
     3241 agatgaccag ccagaaagat cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc
     3301 tggatgatgg tcgcctgagc tatagcctgg aacaaagtct ggatgacgat aacaaccata
     3361 acagtagcct gaacgccagt taccgttcac cttatggcac cttcagtgcc ggatacagct
     3421 acggtaatga cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc
     3481 atggcgtgac gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaatgggg
     3541 cttctggcgt gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag
     3601 tggttcctta tctcacgact taccaggaaa accgtctctc ggtagatact acgcagctgc
     3661 ccgataacgt cgatcttgag caaacaacac agtttgtggt gcccaacaga ggtgcaatgg
     3721 tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca
     3781 acggtaaacc gttgcccttt ggcgctcttg ccagcaacga tgagacgggg caacaaagta
     3841 tcgtcgatga gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga
     3901 ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt
     3961 cagaaccaac aacctctgta ttacaaggca cggcgcagtg ccattaagga taaaaaaatg
     4021 aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc
     4081 tcaggagggt gtggagctga cagcactagc ggtgcgacaa attacagcag tgtggttgat
     4141 gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc tgccacgcta
     4201 agtagcacta actggcaata cgcctgtacc tgctctgcgg gtaaggcagt taaacttgtc
     4261 tatatggtca gccctgtact taccaccact ggacatcaag caggatatta caaactcaat
     4321 gatagtctgg atattaaaac cacattacag gcgaatgaca ttccaggact cgtgactgac
     4381 cagaccgttt ctgttaacac ccgattcaca cagataaaaa gcaacaccgt atattctgct
     4441 gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt
     4501 gcaaatacca cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg
     4561 aaaacggata ttgccgtcat taaaggcgca tgggtcgatg gaatgggaag cccgtctaca
     4621 ggtgacttcc atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg
     4681 tgcaaaatta atcagggcga tgttattaag gttaattttg gattcatcaa tggtcagaag
     4741 tttaccaccc gcaatgccat gccagatggt tttactccag tagactttga tatcacttat
     4801 gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact
     4861 ggggtagtag accagtacaa cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat
     4921 gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt
     4981 atccttcccg ttgatccttc cgggcatggc accatcaaca tgcgcgcctg gccagttaat
     5041 ctggtcggtg gtgagctgga aacaggaaaa tttcagggca ccgccaccat taccgtcatc
     5101 gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg cttcggcact cttacccctg
     5161 ctgctccagc gcatacttat acagcgcatt tttcttcacg ccgtgaattt ctgccgccag
     5221 cgccgccgct tttttcaacg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc
     5281 ggcgggtaag tcttcttcct gtgctttatg accttcgata atcagcacca tttcgccttt
     5341 gcgacggttt tcatcttctt ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat
     5401 ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac
     5461 cgcaacgata tcttccaggc tatctaacag acggtgggtg gattcataaa aaatcagcgt
     5521 gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa
     5581 aaagccttcg taacagaaac ggtcagaggg taaacccgcg gcgcttaacg cagtgatagc
     5641 agcacacggc ccgggtagcg gcaccacgca aatccccgct tcacggcagg tacgcaccag
     5701 atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttctg
     5761 cccttcttgc agcttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag
     5821 cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc
     5881 ctcggcggca atcagatcaa cggcctgtaa tacctctaac gcacgctggg tgatatccgc
     5941 cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg
     6001 gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaat
     6061 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     6121 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     6181 actgcttata tgcagggcac gacgcaggct gattctgcct tttatcttca gcagatgcag
     6241 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     6301 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     6361 tctcagcgtc gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt
     6421 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     6481 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     6541 cgcgcgttaa ttgctcagga accgctgctc ggcgcgaaag aaaaacagca gaatattgat
     6601 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     6661 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     6721 aacgatcccg acatgatgaa agccgggatc gccgactggc aaaaacgtta tccgaacaat
     6781 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     6841 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     6901 attcagcaag gctttgaagc ggcgaaaaat atcggcactc agccagtggc ggctcaggta
     6961 gctgccgcac ctgccgcaga cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt
     7021 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg aacagcctgc ggcacagcca
     7081 gcgcctgtaa gcaccccggc agcaaccacc acagcggtaa gcgcgccagc gaatccatcc
     7141 gcagaactga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     7201 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     7261 cttaagagca acaccccgct gaacgtgctg gcactgaacc agccggagaa tatcgaaaac
     7321 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     7381 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc
     7441 gatcgcgtag ccaatgcgtt tgcgcaggag tggcagaaac tgggcggcgg caccgttctg
     7501 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc
     7561 gctttaacgg gtaccccgat tactcccaga gcgacaaccg atgaccagtt caccaataat
     7621 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     7681 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagctcccgc
     7741 agtgcgcaag ggaccgctgg cccggatttc cgactggaga tggaaggctt gcagtacagc
     7801 gaaatcccga tgctggcagg cggtaatcta ccgttaatgc agcaggcact cagcgcggtg
     7861 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     7921 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     7981 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     8041 ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagtacaag
     8101 cgcgtcgctg gctggaaggc aaaggactgc gatttgtcgc cgctaacgtg aacgagcgtg
     8161 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     8221 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     8281 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     8341 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     8401 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     8461 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     8521 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     8581 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     8641 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     8701 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     8761 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgtaaaagcg
     8821 gtagaagctg ccgttacgcg tgatatgact attgtggcat tgaccggcta tgacggcggc
     8881 gaacttgcag gtttgttagg gccacaagat gtggagatcc gcattccttc gcatcgtagt
     8941 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     9001 acgcttttcc ctcaccagga tgtttaagga gaatacatga aggcattatc gccaatcgca
     9061 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     9121 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     9181 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaagcc
     9241 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     9301 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     9361 aacgagattc gtcagggcca gccgattggt ctgggcgaag cgtctaacga tacgtggatc
     9421 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     9481 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     9541 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     9601 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     9661 tacaacgatg caaactgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     9721 tcaaatcaga acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc
     9781 cctgtcagcc ataaggcttt cgctgggaac gctttgcgca gcataatcag tgacggcaaa
     9841 ctcaccgccg ggagcgtcat caacaatgcc agcgccggag cagttcccat acctgccagc
     9901 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     9961 accgccatcg ccatcaccca catcaggctg ttatcgacag caccatcggc atgggggaat
    10021 aaccagacgc gagcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
    10081 cagaaaagcg tccatagcgc cctgccccag cggctaaaaa aaccgccctg tgcttccggt
    10141 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
    10201 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
    10261 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
    10321 aatgccagcg caccgcccat cgacacctgt tggcgacgca ttcccgccgc gaccggagcc
    10381 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
    10441 aagcgcgatt gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
    10501 gaaccgagga tcacccccag cactgccgct ttccataccg cgaggaaata gatcatcgcg
    10561 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
    10621 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
    10681 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
    10741 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
    10801 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
    10861 tctgaagagc ttaaaatcgt cacaccttca tgcccgggcc gcatcgcctc tgccagcatg
    10921 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggtaataa acggaacagc
    10981 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg
    11041 cgagcaatgg tcaatttcgg ccagttctgg gcgattaatg cttcttccat ctcccctttg
    11101 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
    11161 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
    11221 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
    11281 atgtcgatgg gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc atggggatta
    11341 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
    11401 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
    11461 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
    11521 caccaggctt aatagaccat gaggtaatta tccccgattg tgggaaattc gcctcatcca
    11581 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt
    11641 gaagattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
    11701 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
    11761 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggc
    11821 ggccattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
    11881 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
    11941 gatgtgattc gggggcgcaa actgaccgcg gttaaaccga tcattattga tgttaaaaac
    12001 gcgggcgcgg aattttacga tcaggaagtc gtggtcgata aagatcagct ggttaccagc
    12061 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
    12121 gccgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
    12181 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
    12241 gtacgccttg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
    12301 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
    12361 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
    12421 gactttctgc gcatcaaaca gataaaaagc attagcgcac cagagttccc cttcctgctg
    12481 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
    12541 cattgctgtt ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
    12601 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
    12661 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
    12721 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
    12781 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaagcg
    12841 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
    12901 ctcgtgatac tcaaccagac cagttacgcc attcaacgcg tcatccgcta aacgatgtac
    12961 ctggaaggcg ctttcagtgc ctggccagcg gcaacgcagg tcgtgatgtg ccgccagttc
    13021 aaagccaaaa cggctgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
    13081 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
    13141 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
    13201 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
    13261 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
    13321 gaatgagcga cgtagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
    13381 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
    13441 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
    13501 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
    13561 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
    13621 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
    13681 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
    13741 ccttcaagaa actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc
    13801 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
    13861 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
    13921 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
    13981 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
    14041 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct
    14101 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    14161 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac
    14221 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    14281 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    14341 tggcagcgtg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    14401 ctcgccatgc tagagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    14461 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgct
    14521 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacaactggc acgggtcaca
    14581 cctgtaccac tggaagtctt tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc
    14641 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg taggcgcgtg ttctccggcc
    14701 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    14761 atcgaccgtt atcaggacgg cgaaaacgca ggttatccga cgctatgtaa agggcgttat
    14821 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    14881 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    14941 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    15001 gccgatccgc aaaacttcat accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    15061 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    15121 aatattcctt agggccagtg ctgtggtact ggccaaaaga gacgctggaa gatttttatc
    15181 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcgata tgcagcaagc
    15241 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagtggca
    15301 agcagattgt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    15361 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    15421 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    15481 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    15541 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt
    15601 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    15661 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    15721 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    15781 ccatgagcgg ctacgtttac aacctcggta acgagctggc atccatgcag gggctggttg
    15841 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    15901 ctaatgaaaa tggcgctgcg ccactaccgt tgacggcaaa cagcgactgt aacggctact
    15961 ggcggcggct ggccgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    16021 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    16081 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    16141 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    16201 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    16261 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    16321 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    16381 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    16441 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    16501 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    16561 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    16621 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    16681 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    16741 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    16801 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    16861 atggtgtgca tcaatattat cgccgccgat agcaaccgcg acgctgagtt tctgtttacc
    16921 tcaatgcagc aagcctttgt gaagttgcgc cgtggcgaaa ccgggcaact gccgccaccg
    16981 attcaaaata tggatcagtt ctggtcgccg tccgaacagt atggtgtgca gcaggcgctg
    17041 agtatgtcgt tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc
    17101 gaaaccgacg ccgatgagat tatggtcaac gggcagattt tcgaccacca ggcgcggctg
    17161 cattcgtttg agctggcgat ggatgttaag gaagagttgt tgggatagtg tgtcttaacg
    17221 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    17281 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    17341 cagtaaatta aagctcgata aaatatggac cagtgcgttg ccaacgccaa acaccagaat
    17401 cagcgtaatc atcggcttgc cgccccagac gcggaatttc gggctgccaa agcgtttacg
    17461 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    17521 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    17581 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    17641 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    17701 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    17761 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    17821 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    17881 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggcttggcac
    17941 gttaccgtga taaccaaacg acgccaggca gaacggcaat gtcatcagca gatacggcgc
    18001 ataagatgca ttgctttctg caacgttgaa caatgtcgca ggctgcacat gccccaacag
    18061 gctgccaaag gtgaggaaga aggtaatgac tttcgccccc agcacaatcg ccgtcatgcg
    18121 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    18181 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    18241 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    18301 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    18361 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    18421 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    18481 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    18541 tgacgtttgg gtggtggtta gtgttgccat gagggcttct ctccagtgaa aaatagtgcg
    18601 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    18661 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    18721 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    18781 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    18841 accactgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    18901 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    18961 gagaatgcga gtacgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    19021 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctgc tgatgtcgcc
    19081 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    19141 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    19201 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    19261 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    19321 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    19381 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    19441 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    19501 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    19561 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    19621 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    19681 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    19741 caggcgttcc agtgtctgtt cacgaagcgc ctggttcatg tcaccgttca gcgcggcgct
    19801 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    19861 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    19921 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    19981 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    20041 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    20101 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    20161 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    20221 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    20281 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    20341 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    20401 ctcaggatca agattctgca gcagaggtaa agagaatgct gcagtttttc cgctccccgt
    20461 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    20521 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    20581 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    20641 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    20701 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    20761 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    20821 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    20881 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    20941 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    21001 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    21061 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    21121 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    21181 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    21241 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    21301 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    21361 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    21421 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    21481 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    21541 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    21601 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    21661 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    21721 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    21781 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    21841 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc cagaactttc
    21901 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    21961 atttgagaga tgtgtaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    22021 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    22081 tcttcgatac gacgaatagc gtgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    22141 gtaccgtcat cttcgatttc gatggtggtg ccagtttctt cggtcagggc acggataaca
    22201 gaaccgcctt taccgataac gtctttgatc ttgtccgggt tgatcttgat ggtatggata
    22261 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    22321 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    22381 ataccttcaa ttttgatatc catctgcaat gcagagatac cgtcgcggga acccgcaact
    22441 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    22501 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gatcggcaca
    22561 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga ggaaccgttg
    22621 gattcagtga tttcagacac aacacgtacg gtgtacggga atttgtccat atccggcatg
    22681 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    22741 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggtg
    22801 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcagt acccagcgtt
    22861 gcggtaacca gcgcctgcgt ttcaccacgg gtgaacagcg cagaaccgtg agtacgcggc
    22921 agcacgccag tacgcacatc cagaccacga atcatatctt tttcacgacc gtcgatacgc
    22981 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    23041 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    23101 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    23161 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    23221 tgccagtccc aacgtggttt accggcttct ttcaccagtt cattgatgtt ctgaataaca
    23281 acctgctgtt gttcatgacc gaacactact gcgcccagca tctggtcttc gctcagcaat
    23341 tcagcttcag attcaaccat cagtacagcg gcttcagtac cagcaacaac cagatccagt
    23401 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    23461 acgcgggcag caccaatcgg accattgaac ggaataccag acagagacag tgctgcggaa
    23521 gcaccaatca tcgcgacgat atccggatta acttgcgggt taacagaaac cacggtggcg
    23581 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    23641 agacgcgcga tcagggtttc gccttcgctt gggcggcctt cacgacggaa gaagctaccc
    23701 gggatacgac cagcagcgta agtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    23761 tgacctggtt tggctttttt ctggccaaca acggtaacga ataccgcggt gtcatccatg
    23821 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    23881 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    23941 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    24001 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    24061 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    24121 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    24181 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    24241 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    24301 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    24361 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    24421 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    24481 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    24541 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    24601 aaccaccagg cgacgaggcg caacgcggcc ttcatcgtca atttcgccca taccgataaa
    24661 tttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    24721 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    24781 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    24841 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    24901 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    24961 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    25021 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    25081 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    25141 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    25201 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    25261 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    25321 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgccagttgc
    25381 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    25441 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    25501 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    25561 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    25621 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    25681 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    25741 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    25801 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    25861 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    25921 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    25981 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cgcatcaggc
    26041 agtccatgca ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    26101 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    26161 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    26221 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    26281 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    26341 aaatttcggt gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    26401 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    26461 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    26521 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    26581 ggtgataccc cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    26641 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatatt
    26701 cacttcgtga acttcgcctt cggtcatgtt ggcaaacatg ttctcgagtt tagatttttg
    26761 ctgacgcgcc agtttaactt cgcggaattt accctgacga tagagtgcaa cttcacgcgc
    26821 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggaacgc cggacaggcc
    26881 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    26941 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    27001 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    27061 aaccgcaccg ctcgccatac ctttacgtac cgcttttagc tccagaactt ccgcctgcag
    27121 caggatagcg tccagcagtt catcgatacc ggtacccgcc ttcgcagata cgtgtacgaa
    27181 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    27241 acgatccgga tcggcttctg gtttatcgat cttgttcact gcaaccacca ccggtacctg
    27301 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    27361 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    27421 agcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    27481 accaatgtgc tgggtaatgc cgcccgcttc gccagaggcc actttcgttg aacgaatgta
    27541 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    27601 cggttcagcc gcagcccccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    27661 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    27721 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    27781 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    27841 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    27901 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    27961 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacggcc
    28021 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagttgtgac
    28081 atgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    28141 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gacgcgcttc
    28201 ttcttcagct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    28261 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    28321 tttcgcttgt tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt cagctttttg
    28381 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    28441 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    28501 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    28561 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    28621 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    28681 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    28741 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    28801 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt
    28861 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    28921 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    28981 atcgtcagcc ggtttgttat caccgaggct ttcttccagg gcctgtgcaa tggtggccag
    29041 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    29101 caacagctct ttcatcggaa cataggccag ttcttccagc gtcgagaaac cttcttctac
    29161 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    29221 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt tgagttccca
    29281 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    29341 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    29401 aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac
    29461 gatatcgata cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat
    29521 acctacacag gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    29581 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    29641 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    29701 cgcgccacgc gcttccgggc gaacggaata gagcacgcca cgaacgcggt cgccagggcg
    29761 gaagttttca cgcggcagca tatcttcgcg caggatcacg gcttcagcat tgttgccgag
    29821 atccaaagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    29881 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    29941 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    30001 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    30061 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    30121 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    30181 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    30241 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    30301 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    30361 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    30421 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    30481 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    30541 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    30601 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    30661 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    30721 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    30781 aaagggctta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    30841 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    30901 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    30961 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    31021 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaag
    31081 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    31141 gtgatccatg ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccaattc
    31201 actttcattt gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca
    31261 aaaaacacac taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat
    31321 tttgcataaa aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg
    31381 tgttatttta tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct
    31441 ttttctggcg gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt
    31501 ccttatgcat atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct
    31561 cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg
    31621 gtggccgaag gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg
    31681 acctatttca acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg
    31741 atgaaagaag atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc
    31801 gaacgtttct atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg
    31861 cttgatactg actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt
    31921 gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg
    31981 cttggtgcaa cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc
    32041 aacccaatta tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc
    32101 acagtacgct ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac
    32161 gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac
    32221 cagattgaaa accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg
    32281 gcactgctgc acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt
    32341 gagcagtatc acgcgcatgg tcgtcagttg ggccgtctgc tgtatcaggg gcgttggttt
    32401 gactcccagg cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact
    32461 ggtgaagtta ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca
    32521 gagaacctga cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg
    32581 ccagatgatc gtattggtca attgaccatg cgcaacctgg atatcaccga tacccgcgag
    32641 aaacttttcg gttatgccaa aactggcctg ctctcctcct ctgccacttc aggcgtgccg
    32701 caggtggaga atctggaaaa taaaggccag taattcgaca tcaaccctgc ccttcagggg
    32761 tggggttacc ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat
    32821 catggtttgc atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctccagcg
    32881 tttgcggctg ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtacg
    32941 cagtggaaaa gatatcgttt tctgttgtgc gatccacgcc atccccaaga acggggctat
    33001 accagataaa catcgggaca tgatatgcct gctggctggc ctccctgcct ccatgaaaat
    33061 agacgttctt cttcgtaggg tcgcgttcca ggccgtggtc ggcaaaatac atgaccgagg
    33121 cgcggcgatc ttttagtaat tcaaaaacct gacctagcaa actgtctgtg taatgaatgg
    33181 agttgtcata gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat
    33241 aggcgctaca agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct
    33301 gcgtcttttg ctgtaacgct tgcgataaat gcggcaacaa caattcatca aatcctctga
    33361 catagactgt ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa
    33421 aagcggattg cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa
    33481 tgttgtcggg gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg
    33541 gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt
    33601 ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt
    33661 cgacacgtac agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg
    33721 ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg
    33781 ctaaagcaaa atagttgaga ttaaaaaacg gcgtataggt agcaaatcgc gacgctagta
    33841 tatatggact gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa
    33901 ataaactgcc cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg
    33961 aaacatcata ttttattatt actgccaaaa aaagaaggga taaaaaagca aaagcacata
    34021 gataagggat atacatcccc agcattttga caacttcatc cggatcactc tgcagcacac
    34081 taatcgcaaa tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac
    34141 tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg
    34201 atgatatacg tttaacaacc aggatggtca ggacgaagaa cagcaatcgc tgaaccatat
    34261 gcgctccgga agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca
    34321 accaatgaga tttaaaactt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt
    34381 tcacaaccaa tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa
    34441 ctctgatcca tatcagaccg aaacactgtt agttttttct ttttaaatga tgcggccatt
    34501 gttctcagta attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca
    34561 tcagtagtta tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg
    34621 taccggaact ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta
    34681 taactaaggc gtaccggcac catcgtttca aagtaccagc tacgagtaaa gcaactggac
    34741 gagatacaga tacctgacaa ccattcctca gaccaggacc aaaacgaaaa aagacgcttt
    34801 tcagcgtctc ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt
    34861 gggcactacc acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac
    34921 tttttagttc gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt
    34981 tttcgccggc gcactcaggt tttcccattc gctaccttta ttggttttat tgctattgat
    35041 gttacccagc accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt
    35101 catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga
    35161 ggctcccata tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc
    35221 cacaataagg aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt
    35281 gccgcgtacc aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg
    35341 gtgtgaatac taaccaaagc gaatgacctt cgcaagggca attttatcgc attgtatcaa
    35401 ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc
    35461 tttttagcca gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca
    35521 aattcagtca cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg
    35581 ccggatttac gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc
    35641 acggctttaa ctgactcatg ctcaagtgga tcgccgctac ctgcggtgta acgtacgtta
    35701 accagaatct gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta
    35761 cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtagtttta
    35821 tccagtagga tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt
    35881 ttttccagta cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc
    35941 agcgccagtt caagccccat gttgctcatc aatgtaccca cagcgccacc acgcagctgg
    36001 ccctgacgaa gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg
    36061 ccttcatggt cgaccataat cacgcgatcg ccatcgccgt cgaaggcaat accgagatcc
    36121 gctttttcag ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca
    36181 ttgatgttta caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc
    36241 agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt
    36301 tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg
    36361 gcatcaacga tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt
    36421 tccatttccg cttcgatggc ctcttctacc gcatccggca gtttggtgcc gtcgatagag
    36481 aagaatttaa tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc
    36541 tctgcgcgga aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag
    36601 agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca
    36661 gaaatacgcg tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta
    36721 cccgcggccc aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga
    36781 ccacgaatcc catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc
    36841 agacagagtg gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg
    36901 aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg
    36961 ctcggacggc accacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa
    37021 cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga
    37081 gagattttta ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc
    37141 ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc
    37201 atacttcgga gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg
    37261 taaaccggtt tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt
    37321 aataatgtga gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac
    37381 cgagatccag acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa
    37441 ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat
    37501 gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt
    37561 atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg
    37621 atggctaagg tccagtgaag taccctgggc aaagagtttc atgatgttat ccctggtatg
    37681 aattgataag aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct
    37741 gatgcgggaa cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta
    37801 cgcggttcat caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa
    37861 gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca
    37921 atctgcggtg cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata
    37981 tccatattgt cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc
    38041 acttcctggt cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta
    38101 cggccgagga atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag
    38161 aagccccact gggtcaccat gttacgtgcc agattggtcg caactttaat atcgttggac
    38221 gcaccggtag aaacatgttc cggcccatag atgatctctt ctgccagacg accaccgtac
    38281 agcgtagaaa tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc
    38341 aagaagaaag tcacacccag cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga
    38401 tcgtgttccg gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat
    38461 tctttctgcg cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct
    38521 ttcgctttct cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt
    38581 gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca
    38641 atgattgccg cgtcgatatc gggggccaat ggtacgcgac gcatgtgaac tttcaggatc
    38701 tgctcacgac cgcgaacatc tggcaagccg accacaacct gacggtcgaa acggccagga
    38761 cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct
    38821 tcgttacctt cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca
    38881 tcgtgaccac cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata
    38941 aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca
    39001 cccacaccga cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc
    39061 gcttcgcctg caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc
    39121 aggacgcctt tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac
    39181 tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc
    39241 gttttgatct gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct
    39301 ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc
    39361 atcgggaacc aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg
    39421 acaaccttga cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg
    39481 taagtggtat aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc
    39541 gcttcacgaa cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg
    39601 ccattagact cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc
    39661 agccagagta ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt
    39721 gttaaaaaca gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct
    39781 cccggttagg gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcacgagaa
    39841 gagtccggct tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca
    39901 tcgaaacctt cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca
    39961 cgacacattt ctaacgccag ttccaccaga tacatggcac gggggatatc caccgccggt
    40021 gttccgctca tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg
    40081 cgctccagca gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca
    40141 ccaacgatag gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt
    40201 tgggtgacca catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt
    40261 cccggtttaa agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa
    40321 cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag
    40381 cggctggagc tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca
    40441 tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta
    40501 gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca
    40561 aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa
    40621 tggtttgacc gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat
    40681 caaggtgaaa atcgccaccg aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt
    40741 gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc
    40801 aactaaagaa cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg
    40861 ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg
    40921 ataaaaggcc gcatagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt
    40981 gggtaattct tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt
    41041 tttgatgacc acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt
    41101 tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg
    41161 ttcttcgtca gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt
    41221 gttgggcatt ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt
    41281 aatacggcct tcgcagaaac cctgctgttc acgagctgcg tggtattcgg cgttttcttt
    41341 caggtcgcca tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga
    41401 tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc
    41461 ttgcatttgt tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg
    41521 ttcggcctgc ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt
    41581 acaagtccca ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga
    41641 agggtcatcg tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg
    41701 ttagcgcgag attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt
    41761 cagtgttcag gccgcgaatg ttgatgagta cgttactcaa ctccccgctg gtgccaacct
    41821 tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca
    41881 gatggcgctg cccgccagta cgcagaaagt gattactgcg ctggcggcgt tgattcaact
    41941 cggccccgat tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt
    42001 acttaagggt gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat
    42061 tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt
    42121 gatagatacc tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat
    42181 gacacaatgc tttagcgctc cgcctgccgc tgccatagtt gaccgcaact gtttctccgt
    42241 ctcgctctac agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta
    42301 ccccgttacg atgttcagcc aggtacgcac cctcccccga ggttctgccg aagcgcaata
    42361 ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt acgctgacgg gatgcctgcc
    42421 acaacgttct gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg
    42481 ggcaattctg aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg
    42541 ccagactcag gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgttgca
    42601 cgatctgctt aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg
    42661 catgataggc catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt
    42721 gcgtcagatc ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg
    42781 ttcagggctt tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata
    42841 cattgcccaa cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga
    42901 cggctctttg cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc
    42961 gaaaaccggt tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg
    43021 gcaacgaatg gcgtttgtac aatatctttc tggctatgca gtagaaccgg cggatcagcg
    43081 taatcgccgt attccgttag tgcgttttga aagccgtttg tataaagata tttatcagaa
    43141 caattagtca aaaagaaacc ccggcacatg gccggggttt tgctgattaa cgcttgtaaa
    43201 tgaactcaac gccttcttcg tcgtcttcgt cccagtcatc atcccagtcc tcatcatcct
    43261 cttcagcaat ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct
    43321 ctggctgttt cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat
    43381 cccagcagag atctttcacg cccagtccac tcgccgcaga gatcagataa tatttatctt
    43441 cccagcccag cgcttcagca atcgctttcg ctttctcttc agcttctgcc ttatccagca
    43501 gatcgatctt attgaacact aaccaacgtg gtttcgccgc cagatcctgg ctgtattttt
    43561 ccagctcgct gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga
    43621 tatcgatgag gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cgaatgccca
    43681 gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct
    43741 tttcgttgtc cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg
    43801 ccactttcgg tttagccgcc gataccgcac gaataaaggt cgatttaccc gcgtttggca
    43861 tccccaacat accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcgc
    43921 ccggcgtacc gttagttttc tgccgtggtg tacggttaac tgacgatttg aaacgggtat
    43981 tgcccagacc gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttggtcatat
    44041 cgcccatggt ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaattg
    44101 tgacgtcttt accgcgctta ccggtacagt cgcggcttgc gccattctga ccgcgctctg
    44161 cacggaaaga tttttcaaaa cgataatcga taagcgtgtt caggttctcg tcggcttcca
    44221 tccatacgtc accaccatca ccgccgtcgc cgccatccgg gccgcctttc ggaatatact
    44281 tttcgcggcg gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg
    44341 cttcatcaac aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata
    44401 ccaccgttgt atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg
    44461 cgcccgcaac cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga
    44521 agaaatcggg tcaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga
    44581 gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc
    44641 catatcctac caaggtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa
    44701 gctgcgctat cactccaggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca
    44761 gtaaaaacag gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat
    44821 aactcaccca aaccgtcgcc gcaccgaccc caaagataac tccccaggtg taatcggtga
    44881 gctttgtaaa tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca
    44941 tcaatgcccc tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa
    45001 ccatcatgcc aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt
    45061 gcaaggatga gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca
    45121 aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa
    45181 gaatggcacc aaggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt
    45241 ccatcacctc cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg
    45301 tgagcgccaa aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg
    45361 catttgcgga ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg taaaaagccc
    45421 cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga
    45481 tgctgataaa tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt
    45541 tagcaaacag agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac
    45601 cacgttgacg aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc
    45661 ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg
    45721 ccatttgaaa tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa
    45781 ccactgacga tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt
    45841 aactttctcg ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa
    45901 aggaacgccg attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac
    45961 agtttcgcca gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg
    46021 gtgttgttta ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac
    46081 cttttcaatg attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa
    46141 acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg
    46201 gtaacgttta tctgtgccat tttttcagta caatcactct atattcctaa ccataaaccc
    46261 taagttgcct ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat
    46321 ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt
    46381 gagcagctta attccgacgt ccagctgatc aatcagttag gctattacat cgtcagcggc
    46441 ggcggtaaac gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag
    46501 ggaaatgcgc atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgctg
    46561 cacgacgacg ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgctgca
    46621 tttggcaatg ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg
    46681 atgaccagcc tcggttcgct caaagtgctg gaagtcatgt cagaagccgt aaacgtcatc
    46741 gcagaaggtg aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac
    46801 tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc
    46861 gggattctgg ctggctgtac gccggaagag gagaaaggcc tgcaggatta tgggcgctat
    46921 ctcggcactg ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag
    46981 ttaggtaaaa atgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat
    47041 gcgatgcatc atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacagggt
    47101 aacggtcgcc atcttctgga accggttcag gaagcaatga acgcttgtgg atctcttgaa
    47161 tggacgcgtc agcgtgccga ggaagaagca gacaaagcca tcgcagcatt acaggtgctc
    47221 ccggacaccc cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat
    47281 cgttaatccc ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa
    47341 aacactaatt cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga
    47401 atattcacac tctttacagg aactttttag agcaataggc catcaggagt atagtgatgc
    47461 ccgacagaag aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa
    47521 gtaatttcat tgactggcat cccgctgaca tcattgcggg tttgcgcaag aagggaacgt
    47581 caatggcggc ggagtctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat
    47641 cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg
    47701 ttatctggcc atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt
    47761 tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg
    47821 gctgccagct ctcagacgat tactcgcctt tcacacgctc aatatttgca cctaaagcgc
    47881 gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg
    47941 tcgtcccttc cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg
    48001 ccataacctg tgcgccagaa agtttttcaa caccgtgaca aataacggta ttgctttcga
    48061 tttcggcatg cgcgcccata cggctaagct ccggcacatg cataaagcgg ttttcaaaga
    48121 ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct
    48181 gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt
    48241 tgccatgcat atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac
    48301 gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc
    48361 cgcgagaaat cgccgccgcc accaggaaag taccagtttc gatacgatcc ggtagcacgc
    48421 gatagacacc gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac
    48481 cgctaatttt cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac
    48541 gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt tgcagcacac atgatggtca
    48601 ccgttgcgcc aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga
    48661 cggaagcttt aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag
    48721 aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc gccaggcagt gaaacttgcc
    48781 cctgaccaaa gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa
    48841 ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat
    48901 tacgttctac tttcgcaccc agctggctta gcagcttcat tgatgtatcg acgtctttca
    48961 gtttcgggac gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag
    49021 gcagagcagc atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct
    49081 gaacacgaaa tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg
    49141 aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat
    49201 acgctttgat cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat
    49261 agaccgtctg ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa
    49321 cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca
    49381 acacgctctg aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg
    49441 aaacatctta gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca
    49501 gacaaggctc gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg
    49561 aaaaaattta acgaggcaga acatcagcag gcaaattata caatttcgcg agggtataca
    49621 ctttgtcgtt taccccctga agcgtcacgt tgttgccctg ctttttcgcc agatcgataa
    49681 gatggagcag cagtgccagt ccacccgtat ccacgcggga cacacggcta agatcgatgc
    49741 aggtaatccc cttcaccgct tcctcacgca tttcccaaag cggtagcaga acgtcctgat
    49801 ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca
    49861 ttattttttc tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag
    49921 gccgtcgata cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat
    49981 actgacgcct tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg
    50041 ccactggaag tccagacgca ccggcggacg gccattcggg tcaataatgg taacgcgaat
    50101 aggcacaatg gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg
    50161 atacatcgcc agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta
    50221 ggcttcacgt tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc
    50281 gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg
    50341 gttggcccga atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc
    50401 gtccatcagc ttataaggat tggtctggtc tgccgcggtt gccgcactca gaggtgcaat
    50461 caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt
    50521 cgttgtaccc acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc
    50581 actattctta ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag
    50641 caccatcgca gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag
    50701 ttccgggtct tcaaaaccga cgtttaatgc cagatattgt tcccccagca ggccggaagt
    50761 acgaatgctc agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt
    50821 tacgcgcggc agataggttt tcgggtccag cgtaatatcc gccacccgac ccacaacaac
    50881 gccaccaata ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata
    50941 aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa
    51001 cagcgccgcc agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt
    51061 ttgcatgaac tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag
    51121 ccagagacga gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga
    51181 tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct
    51241 taatcagaca gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga
    51301 agaacccgct atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca
    51361 cggcgacgaa aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag
    51421 aaataacccg acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag
    51481 cgcgcatcag gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg
    51541 cggcaaccac cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg
    51601 tttccgcact ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga
    51661 acacgccaga aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca
    51721 ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta
    51781 acccggcccg cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg
    51841 catttaacag catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca
    51901 ggatagcgga acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga
    51961 tcaggattcg cctgcaacgc ctgggcactg ccatgagcga caattttttt gtccgccagg
    52021 atccaggcgt gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa
    52081 gtcacgccca gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg
    52141 ggatcttgcc caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca
    52201 cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt cagaaggcat tagcttagcc
    52261 gctccacgca gccccacggc ctccagcttc atcatcactg tactatgcaa caatggcgcg
    52321 ggaagttggg tatgttcgcg cagaggatag gcgacgttgt caaatacgtt catatcagtg
    52381 aacaacgccc cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa
    52441 cgagacatcg ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt
    52501 tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgattgcc
    52561 gtgatcttcc ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc
    52621 gtaaaactga catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct
    52681 tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta
    52741 cagaatatta cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata
    52801 aagtcaaaat taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata
    52861 cctgaagaaa ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt
    52921 ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg
    52981 catcccgccg ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat
    53041 catcgtctcg cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct
    53101 cggctcaaac attatcaata tattgctgat cctcggtctg gctgcgctgg ttcgtccttt
    53161 taccgtccat tctgatgttc tacgccgtga attaccctta atgttgttgg tcagcgtggt
    53221 ggccggttcc gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt
    53281 tctggctgtg ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg
    53341 gactgacagc ctgaccagag agcagcttgc agagctgccg cgtgacggcg gattgcccgt
    53401 cgcgttttta tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt
    53461 tgataacgcc acggtgctgg cgaattactt tgccatcagc gagttgacga tggggctgac
    53521 ggcaattgct atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa
    53581 aggtgaaaac gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat
    53641 cgtgttgggt ttacccgcgc tgataacgcc aggagagatt gatccactgg cgtacagtcg
    53701 tgactacagc gtgatgttgc tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc
    53761 cccgcaaccg ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc
    53821 gatgttgtac tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg
    53881 tagagttaca accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac
    53941 gtgaatgcct ggcggagctt gagcaataca tcaatcagaa tttcacgctt gcttgtgaaa
    54001 agatgttctg gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcacatcg
    54061 ggcgcaaaat ggcggcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg
    54121 gtgaagccgc gcatggtgat ttaggcatgg ttaccccaca ggatgtggtg attgctatct
    54181 ctaactcagg tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg
    54241 taccgttaat ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc
    54301 atctgtgtgt taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca
    54361 ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct
    54421 ttactgctga agattttgcg ctctcacacc caggcggcgc actgggtcgt aaacttctgc
    54481 tgcgcgtaaa cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca
    54541 gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg
    54601 atgacaatat gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata
    54661 tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat gacgccgggg ggaatacgtg
    54721 tgcgccctgg cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct
    54781 ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc
    54841 gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct
    54901 tgcgacctgt tacggccctg tcagcgccga cgttatggca aaagcagaga acattcgtct
    54961 gctgatcctc gacgttgatg gcgtactgtc agatggcctg atttatatgg gcaataacgg
    55021 cgaagagctg aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc
    55081 tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc
    55141 cacattgggg atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga
    55201 tttgctggaa aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat
    55261 cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg cgcatccact
    55321 gttgatcccg cgcgccgatt acgtgacgcg cattgctggc ggtcgtggcg cagtgcgcga
    55381 agtttgcgac ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat
    55441 atgagtaaag ccagacgttg ggttatcatt gtgctatcac tggcggttct ggtgatgatc
    55501 ggcattaata tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc
    55561 acctataaaa gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat
    55621 cgattgattg ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag
    55681 ccggtactta ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa
    55741 gccaagctga ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactc
    55801 gtgccggact ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg
    55861 caggatgtta cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt
    55921 ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca
    55981 tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct
    56041 ggccgccagc attccggcat ttgccgtaac cggagacact gatcagccga tccacattga
    56101 atcggaccag caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat
    56161 cgtcacccag ggcaccatca aaattaatgc cgacaaagtg gttgttaccc gtccgggcgg
    56221 cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca
    56281 ggacaacggt aaaccggttg aaggtcacgc ttcccagatg cactacgaac tggcaaaaga
    56341 ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt gatagcaaca ttaagggcga
    56401 taagatcact tacctggtaa aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg
    56461 cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc
    56521 ggcacagaag aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa
    56581 agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat
    56641 tgtcggtctg ctggggccaa acggtgccgg taagaccacc actttctaca tggttgtagg
    56701 cattgtgccg cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc
    56761 tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca caggaagcct ccattttccg
    56821 tcgcctcagc gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc
    56881 tgaacaacgt gaagaccgcg cgaacgagct gatggaagag tttcacattg agcacctgcg
    56941 tgacagcatg gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc
    57001 actggctgcg aatccgaaat ttattctgct cgacgaaccg tttgccgggg ttgacccgat
    57061 ctcggttatc gacattaaac gcatcattga gcacctgcgc gacagcggcc tgggcgtgct
    57121 gatcactgac cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag
    57181 tcaggggcat ttgatcgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa
    57241 gcgtgtatac cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag
    57301 caggagagta cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg
    57361 gcgatgacgc cacagctcca acaggcaatt cgtctgttgc agttgtcgac gctggaactt
    57421 cagcaggagc tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat
    57481 gaagaaatcg acacccgcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc
    57541 gaacaaaaag agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc
    57601 gctggtacac catccggcac cagcggtgac tacattgacg atgagctgcc ggtctatcag
    57661 ggcgaaacga cgcagacctt gcaggattac ctgatgtggc aggtcgagct gacaccgttt
    57721 tccgacactg accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat
    57781 ctgactgtcc cgctggaaga tattctcgaa agtatgggcg atgaagagat cgacatcgac
    57841 gaggttgaag ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa
    57901 gatctgcgtg actgcctgct aatccaactc tcccaattcg ataaaaccac gccgtggctg
    57961 gaagaggcca gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc
    58021 actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc
    58081 cagtcgctcg atccgcgccc cgggcagtcg atccagactg gcgaacctga gtatgtcatt
    58141 ccagatgtgc tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc
    58201 attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat
    58261 gatgacagcc agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg
    58321 gaaagccgta acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc
    58381 ttctttgagc aaggtgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct
    58441 gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca
    58501 cgaggcattt ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc
    58561 gaagcttcct ccacggcgat tcgtgcactg gtgaagaaat taatcgcggc ggaaaaccca
    58621 gcgaaaccgt tgagcgacag caagttaacc tctttactgt cggaacaagg tatcatggtg
    58681 gcacgccgca ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt
    58741 aaacagctcg tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa
    58801 taacgtcgag atcaccgagg cactgcgcga atttgttaca gctaaatttg ccaaacttga
    58861 gcaatatttt gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca
    58921 cacctcagat gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca
    58981 ggacatgtac gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca
    59041 taaagataaa ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt
    59101 tgtgcggcac aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt
    59161 gggcaggttc ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt
    59221 tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag agcaaaaaac gcgccctgga
    59281 aatcatcagc gagctggcgg cgaaacaact tagcctgccg cctcaggtgg tttttgaagc
    59341 tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca
    59401 tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaagcgcc
    59461 tatagctttc gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc
    59521 ggcagaccaa actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga
    59581 caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat
    59641 tacggatacc gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg
    59701 ttgtgaggag aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa
    59761 atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt
    59821 agtgttgtta cccgatctgg ctcgaactct ggccgatcgc gagatttctg ccgccgtcag
    59881 cattgatgtt cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct
    59941 gcctgacgct ttctcaccgc aactactgtt cctggatgcc gaccgtaata ccttaattcg
    60001 tcgttacagt gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag
    60061 tgctatcgac aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt
    60121 cgatacctca gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg
    60181 taaacgtgaa cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc
    60241 tatcgatgca gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa
    60301 actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga
    60361 agtacacaat tttatctacc agacgcgaag ctatcttgaa ctatggttac ctatgctgga
    60421 aaccaacaac cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc
    60481 ggtgtatatt gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc
    60541 acgccatcgt acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca
    60601 aacaagctgg gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcagggtttt
    60661 gacgctgaag tgctgttacg taatgacgaa ggcaccgagg ccgaagccaa cagcgttatt
    60721 gcgctgctga tgttggattc tgccaaagga cggcagattg aagttgaagc gaccggccca
    60781 caggaagagg aagcactggc cgccgttatc gccctcttta actctggttt tgatgaagat
    60841 taatcttcat cactttgaca tacaactccc ttcaaactcc ccccgacaat aagaaaatca
    60901 cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag
    60961 aagaacctta agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat
    61021 actggagatc gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt
    61081 cccctgggca ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt
    61141 atcaagattg tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag
    61201 tactacgcgc atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt
    61261 accgttgaga cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggt
    61321 aaaccctcca ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca
    61381 caactgcgcc agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg
    61441 atgtcgctga agccgcagaa tatcctctgt caccgcatca gtgagtcaga ggttatcccg
    61501 gtcgtctgtg acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg
    61561 tgttgtctgc gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct
    61621 attgccctgc agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc
    61681 gaagcttaat ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc
    61741 cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga
    61801 agtggattag gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg
    61861 ggtttgtgga aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg
    61921 gcgatattca ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc
    61981 gttaatccag cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt
    62041 ttggccgtct gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg
    62101 tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga
    62161 tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag
    62221 tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc
    62281 attaccgctg agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa
    62341 acggcgagga caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag
    62401 cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg
    62461 ccgcttctgc aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt
    62521 catcaaccac gatatcatcg acaggacagg gcacatgctc cgcgcccatc tcttccagca
    62581 cttctgcggt atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg
    62641 gcagcatcgc cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt
    62701 gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagagtg gcaaaattgc
    62761 ttaaattctt cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat
    62821 cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacgt
    62881 tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat
    62941 ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg
    63001 cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct
    63061 tcattgtgat catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt
    63121 acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg
    63181 cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag
    63241 ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt
    63301 ttagtggctt ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc
    63361 atctcttcaa tccattcacc gacgttatct tcccaggcag gaaggtccgg agactgaatt
    63421 tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta
    63481 tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg
    63541 acatagcccg gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct
    63601 acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc
    63661 tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc
    63721 gcggttagcg ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg
    63781 tactcttgtt tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg
    63841 cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga
    63901 atatccagca acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg
    63961 gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg
    64021 ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca
    64081 tcaaacgcat catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt
    64141 gaacctttgc cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga
    64201 cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc
    64261 acctggtaat acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag
    64321 tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg
    64381 ccttgctggg tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc
    64441 gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg gctccaggtt aaagcacaat
    64501 cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa
    64561 tcaaccggtt gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata
    64621 tcgttaaaga tattccccag cgtgacggcc gaaacatgga tggtcttgag atatttttcc
    64681 tgctcggcgg tgagttcggt atccagcaga atacggctca ggccgacgat accgttcagc
    64741 ggtgtacgca attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcccgt
    64801 tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg
    64861 tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc
    64921 ccgtccgggt aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt
    64981 tcatcggttt caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg
    65041 tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa
    65101 aactctttat cttcgttacg ataaaaaacc aggtcgggtg aagcatcaag gaaggaacgt
    65161 aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt
    65221 ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc
    65281 tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc
    65341 atctcctcca gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg
    65401 acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa
    65461 cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc
    65521 acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac
    65581 ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc
    65641 cttcacgaca acctgcccca ataataccca attctgacag ttacgttata aattgtgtga
    65701 gaaatgcgga attcccctca acgacctact ccgttggagg tatccacgga cgttccagcg
    65761 ccgatccctg tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc
    65821 gattttcgca ccacagagga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt
    65881 ggtagatcac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct
    65941 ccagttcaat accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca
    66001 caatatgcag cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg
    66061 tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct
    66121 tcagcccgcg ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc
    66181 ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt
    66241 cgtaaccctg gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg
    66301 ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag
    66361 aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc
    66421 gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct
    66481 gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg
    66541 taccgtcacg gttagggcag ctaaaaccgc cgtgtagcgt cagcttatgc accttttgcc
    66601 cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct
    66661 gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga
    66721 cctggatcaa tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa
    66781 tttcatgcat aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct
    66841 tatggatttt aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa
    66901 tgctggtttt ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag
    66961 gaattgactg tttctctaac gacttccctt ttagccttaa agataaaatc cattttaatt
    67021 tcagtaattt aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat
    67081 ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca
    67141 gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg
    67201 aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac
    67261 gctctttctg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag
    67321 gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc
    67381 gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc
    67441 cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg
    67501 ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta
    67561 gcaaaaaact acgccgtcgg gatgctattc ctgaataaag atcctgaact cgccgctgcc
    67621 gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt
    67681 gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt
    67741 gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc gcgatatgga gcgccgtctg
    67801 tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt
    67861 agcctgtcga atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc
    67921 ttttatctgg atcttgcgga cctgcgtctg gaatcggcca tctgcctgtt ccaccagcgc
    67981 ttctccacta acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctggcgcat
    68041 aacggtgaaa tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa
    68101 ttccagacgc cgcttatccc tgatctgcac gacgccgcac cgttcgtcaa tgaaaccggc
    68161 tctgactcca gttcgatgga taacatgctg gaactgctgc tggcaggcgg aatggatatc
    68221 atccgcgcca tgcgtctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg
    68281 gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggcg
    68341 ggcatcgtga tgtccgacgg tcgttttgcc gcctgtaacc tcgaccgtaa cggtctgcgt
    68401 ccggcgcgct acgtcatcac caaagataag ctcatcacct gcgcctctga agtcggtatc
    68461 tgggattacc agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg
    68521 gttatcgaca cccgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa
    68581 agccgccatc catataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa
    68641 gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct cgccagctat
    68701 cagaaacagt ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa
    68761 aacggtcagg aagcggtcgg ttcgatgggc gacgacaccc cattcgccgt gctctccagt
    68821 cagccgcgca ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca
    68881 atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg
    68941 aacgtctttt gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg
    69001 ctctactccg atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg
    69061 ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctgtgc
    69121 gacaaagccg aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat
    69181 atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg ttggcgcgat ccagacccgt
    69241 ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc
    69301 gatccgcacc acttcgccgt gttgctgggc ttcggcgcga cggctattta tccatacctt
    69361 gcctatgaaa cgctgggccg cctggtagac acccatgcga ttgccaaaga ttatcgtacc
    69421 gtgatgctca actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg
    69481 ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac
    69541 gatgatgtag tgggcctgtg cttccagggg gcggtcagcc gcattggtgg ggcgagcttt
    69601 gaagacttcc agcaggatct gctgaacctg tcgaaacgtg cctggctggc gcgtaagccc
    69661 atcagccagg gcggcctgct gaaatacgtc cacggcggcg aataccacgc ttacaacccg
    69721 gacgtggtgc gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa
    69781 tacgcgaagc tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg
    69841 ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc
    69901 tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg
    69961 gaagcgatga atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg
    70021 cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt
    70081 actccggcgt atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag
    70141 ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc
    70201 tattcggtgc ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc
    70261 gaggacttag cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc
    70321 gtgaagctgg tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat
    70381 gccgatttaa tcaccatcgc aggctatgac ggtggcaccg gcgcaagccc gctgtcgtca
    70441 gtgaaatacg caggttgccc gtgggagctg gggcttgttg aaacccagca ggcgctggtt
    70501 gccaatggtc tgcgtcataa gatccgtttg caggtcgatg gcggcctgaa aaccggtgtt
    70561 gatatcatca aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccgatg
    70621 gtggcgctcg gctgtaaata tctacgtatt tgccatctga acaactgcgc aacgggtgta
    70681 gcaactcagg atgacaaact gcgtaagaac cactatcacg gcctgccgtt caaggttacc
    70741 aattactttg aatttattgc ccgtgaaacc cgcgagctga tggcacagct gggcgtaacg
    70801 cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag aactggacgg tttcaccgcc
    70861 aaacagcaaa aactggcgct gtcgaagctg ctggagaccg ccgaaccgca tccaggtaag
    70921 gcactctact gcaccgaaaa caacccaccg tttgataacg gcctgctgaa cgcgcagttg
    70981 ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatt
    71041 cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc
    71101 gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt
    71161 ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat
    71221 gtcggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc
    71281 cgcagccatg aagcaagtat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt
    71341 ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc
    71401 gtggtagaag gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt
    71461 ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg gcggcttcgc ttacgttctc
    71521 gatgaaagcg gtgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaagcgtt
    71581 gacgatctgg cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat
    71641 actggctctc agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt
    71701 gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcatcg tagtcgtagc
    71761 gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat
    71821 caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgcaaaatt
    71881 gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc
    71941 tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc cggtacacaa ctacatcccg
    72001 aactggctga agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag
    72061 accaacaccc tgccggaagt ttgcggacga gtctgcccgc aagaccgtct gtgcgaaggt
    72121 tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg gcaacattga gcgttatatc
    72181 aatgataaag cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt
    72241 aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg
    72301 cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc
    72361 ttcggtattc cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc
    72421 actggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac
    72481 gatctgctga gtgattacga tgccgtcttc cttggcgtcg ggacttatca gtcaatgcgc
    72541 ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc
    72601 aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa
    72661 ggcaaacgcg tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgtacgtcc
    72721 gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc gtgatgaaga taacatgccg
    72781 ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc
    72841 cagccactgg ggattgaagt gaacggtaac ggtaaagtca gcggcgtaaa aatggtacgt
    72901 accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc
    72961 gaacatatcg ttccggcaga tgcagtgatc atggcgtttg gtttccgtcc acacaacatg
    73021 gaatggctgg caaaacacag cgtcgagctg gattcacaag gccgcatcat cgccccggaa
    73081 ggcagcgaca acgccttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc
    73141 cgtggttccg atctggtggt aaccgctatt gccgaaggtc gtaaggcggc agacggtatt
    73201 atgaactggc tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg
    73261 ctgaaattct cctttctttc tggaaagcgc ctcgcataat ctgaagaaaa ccaatgtaat
    73321 acgctcatct ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg
    73381 cagcgccata taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac
    73441 cagcaaatcc acgacggtat tattcatctg gtcgatagcg cccggacgga aacggtacgt
    73501 agcgttaacg cgctgatgac cgcgacatac tgggaaattg gccgacgaat tgtcgaattt
    73561 gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc taatcaagcg actatcaaag
    73621 gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa acttacgcca aatgaggctt
    73681 ttttacctct tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca
    73741 ttgggaattc cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg
    73801 tctgccaaat ctttccccct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    73861 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    73921 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    73981 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcagca ggcgatacgc
    74041 gatccctttg tgctcgaatt tctggaattg aaagatgaat actcagaatc cgattttgag
    74101 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    74161 gttggtcgac agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    74221 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    74281 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    74341 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    74401 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    74461 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    74521 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    74581 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    74641 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    74701 attcccggtt ttaagatgat ggtttgctcg ttttcaatgg tgctgcaaag ctgataatca
    74761 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    74821 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    74881 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    74941 tcgtaacgac ccggcgcttt ttcttgcggc ctggcggcta atgccagcgt taacgcgtcc
    75001 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    75061 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcatggcgg tagtgcgccg
    75121 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    75181 gatacccttc tgccagacca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    75241 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcgatggatc agccattgcg
    75301 cttgttcgtc gccctgcccg gcgcgtgtaa aaatagttct cgcatccgcg cccgccagtt
    75361 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcttccacg caacctgtgc
    75421 gtccacagcc gcagactgga ccatgcggat cggcaagcgt atgcccgata tgccccgcca
    75481 gaccgccagg gccggtaagc agtttaccgc cgctcactac accgccgccg acgccagtgg
    75541 aaacggtgat aaacaccatc tcggttacat cgccttccag cgcctgatac tctgcccatg
    75601 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    75661 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc
    75721 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    75781 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    75841 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    75901 cgccgatatc aatcgccagt gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    75961 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    76021 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca
    76081 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    76141 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    76201 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    76261 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    76321 gcccgcctgc gccagcgcat cgacatcttc aatataggcc gtgatgcgta ccggagaatc
    76381 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    76441 cagatttgcc acaccttcaa tacgaatggc gaccgcgccc gcctgctctg ccgctaatgc
    76501 catggcggcg acgatttcgg gtttatcgag cgggctgtca ggaaccggct ggcaggagac
    76561 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    76621 tcccttagcg aaaggcccgg tacagagacc gggcaacagg attaactttt ggttttgact
    76681 aaatcgtttt tggcgctgcc aaacggcacg gcaccgctga atggtttacc gtcgatagcg
    76741 tcatgagtac gcaacgcttc cgggcgcaac caacgctgaa cgcgagaagg catatccagc
    76801 ccaatcagca ggatcaccac gaacgtcaga ctgaacgaga gcgatgccag cgcagtaccc
    76861 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcacc gcccaatgcg
    76921 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    76981 atcagttttg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    77041 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat cagcagctgc
    77101 gaggccagca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagcca
    77161 cctacgcagc atcccaccgc cgcgccaaag ccactaaaga acagcacatt ggctacagta
    77221 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    77281 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgtttg
    77341 cctgtactct gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    77401 acgatcgcgg cattttgcag gttaccggcg aagcagaacc acagcgcagt agccgccgcc
    77461 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc caccattgtg
    77521 cgtactggtg ctttacctgc gtgtttctct ttccagtctt ccgcttccgg gatgttttta
    77581 cgcagccaga gagcaaagat gattggcaaa atgccgataa agaacagcgc acgccagccc
    77641 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    77701 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    77761 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    77821 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    77881 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    77941 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    78001 ccgaattcac cctgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    78061 agcagatatc ccaaccaggc agcggaaaat gcgcgccatt gtgcacggtt gagatggcga
    78121 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    78181 acacgctttg gtataaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    78241 ggcgagcgag gggaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc
    78301 agttctggca gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa
    78361 acgacatcca tataatggag gacagttttc aggccgcgga atacgcccgt tttgatcagt
    78421 aaatcaatga ctttattgca ttcagtttgc agtttctgcg cggtctggat atcgccttct
    78481 ttcagcgcct taacgatccc ctgatagcgc cagcccatga tgttgtaggt actaccgata
    78541 ccaccatcag cgcccgccag cagaccagag gcgaagattt cgtcgtaacc gttatagagc
    78601 acaagatcag gatgttcacg acggatctgc tccatctgat agagatcgcc agaggtctgt
    78661 ttcagcgcac ctacgccagg caatgtaaca agtgtgttga tctgatccag ggtcagtttt
    78721 accccactca gggctggaat gttgtacacc accatcggca aaccatccgc cgaatcaata
    78781 attgcccgat agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg
    78841 gcggagacgg catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg
    78901 gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg
    78961 atttccagta cctgttcacg ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca
    79021 cccacgtata aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc
    79081 gctttatcca gtgcttgttg ttggtcaaaa ggagtcagga gtgcagccat tacgccacgt
    79141 aaattcgttg ccataaatac ctctgaagtg atgcttgtct gataaacgat atacctttat
    79201 acctgttata ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc
    79261 ttcactaaag tggcattatt tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc
    79321 agagacgctg ttgagatgcg attgcaacgc acgatcggct tcgtcaggat catgacggcg
    79381 gatcgcatca acgatcgcaa tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc
    79441 ctgatcggta accgttgggc gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc
    79501 catgaagatt gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg
    79561 aatgaatgcc gcgttgttat ccagcgactg actgttgatt tccagtgctt ttgccagcaa
    79621 atcgatttgc tcatcggtgg catgttcagc cgcatagcgc accaggctgg attcaaagaa
    79681 cagacgtaat tgttcgaaat gggcaatccc accaggatga gaaaggaaat ctttcgccat
    79741 gccggaaagc tcgccgatga tggtgtccgc agaaggacgt gagacgcggg cacgttcgcc
    79801 gttgttgatt tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcacagaagg
    79861 acgcccgacg ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc
    79921 aaattcacga cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt
    79981 acgcgccagc gggcggctac gcaagttgcg accaattgca ggtgaagaat cttcggtttg
    80041 cgaatcaaat gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg
    80101 acatcctatc acaggattga aagtagggga aaatggcagg tttttctctt tgtgcctcat
    80161 cattaccata attaacggaa taattaacta ttgcgaaaaa ttaatgtaac gcagat
//
LOCUS       DAZGCS010000005        80197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGCS010000005 DAZGCS010000000
VERSION     DAZGCS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.005"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1597
                     /locus_tag="ACHK01_000339"
     CDS             <1..1597
                     /locus_tag="ACHK01_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO8095343.1"
                     /translation="DNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDK
                     ALAAGAAEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANIS
                     QPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQSETEQNDQKAEQNSPNA
                     LQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQETFDEESQVEAK
                     ENDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAG
                     PGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAP
                     EFNRRTNSGKEEEKAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGH
                     AESSIYWEDPETAILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFY
                     SDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCL
                     NTDEWPAIKTLSLPRWAKEYAND"
     gene            1590..2399
                     /gene="recT"
                     /locus_tag="ACHK01_000340"
     CDS             1590..2399
                     /gene="recT"
                     /locus_tag="ACHK01_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO8095344.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            2455..2604
                     /gene="ralR"
                     /locus_tag="ACHK01_000341"
     CDS             2455..2604
                     /gene="ralR"
                     /locus_tag="ACHK01_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO8095345.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            2642..2830
                     /locus_tag="ACHK01_000342"
     CDS             2642..2830
                     /locus_tag="ACHK01_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO8095346.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            2930..3145
                     /gene="xisR"
                     /locus_tag="ACHK01_000343"
     CDS             2930..3145
                     /gene="xisR"
                     /locus_tag="ACHK01_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO8095347.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            3147..4382
                     /gene="intR"
                     /locus_tag="ACHK01_000344"
     CDS             3147..4382
                     /gene="intR"
                     /locus_tag="ACHK01_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8095348.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            4434..5369
                     /gene="ttcA"
                     /locus_tag="ACHK01_000345"
     CDS             4434..5369
                     /gene="ttcA"
                     /locus_tag="ACHK01_000345"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO8095349.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(5498..6871)
                     /gene="dbpA"
                     /locus_tag="ACHK01_000346"
     CDS             complement(5498..6871)
                     /gene="dbpA"
                     /locus_tag="ACHK01_000346"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO8095350.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(6901..7074)
                     /gene="ynaL"
                     /locus_tag="ACHK01_000347"
     CDS             complement(6901..7074)
                     /gene="ynaL"
                     /locus_tag="ACHK01_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO8095351.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(7349..8332)
                     /gene="zntB"
                     /locus_tag="ACHK01_000348"
     CDS             complement(7349..8332)
                     /gene="zntB"
                     /locus_tag="ACHK01_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO8095352.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(8346..8393)
                     /gene="ydaN"
                     /locus_tag="ACHK01_000349"
     CDS             complement(8346..8393)
                     /gene="ydaN"
                     /locus_tag="ACHK01_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO8095353.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            8587..9819
                     /gene="dgcM"
                     /locus_tag="ACHK01_000350"
     CDS             8587..9819
                     /gene="dgcM"
                     /locus_tag="ACHK01_000350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO8095354.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(9840..10403)
                     /gene="smrA"
                     /locus_tag="ACHK01_000351"
     CDS             complement(9840..10403)
                     /gene="smrA"
                     /locus_tag="ACHK01_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO8095355.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(10733..11641)
                     /gene="abgR"
                     /locus_tag="ACHK01_000352"
     CDS             complement(10733..11641)
                     /gene="abgR"
                     /locus_tag="ACHK01_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8095356.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            11817..13127
                     /gene="abgA"
                     /locus_tag="ACHK01_000353"
     CDS             11817..13127
                     /gene="abgA"
                     /locus_tag="ACHK01_000353"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO8095357.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGM
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            13127..14572
                     /gene="abgB"
                     /locus_tag="ACHK01_000354"
     CDS             13127..14572
                     /gene="abgB"
                     /locus_tag="ACHK01_000354"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO8095358.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            14606..16132
                     /gene="abgT"
                     /locus_tag="ACHK01_000355"
     CDS             14606..16132
                     /gene="abgT"
                     /locus_tag="ACHK01_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO8095359.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            16143..16658
                     /gene="ogt"
                     /locus_tag="ACHK01_000356"
     CDS             16143..16658
                     /gene="ogt"
                     /locus_tag="ACHK01_000356"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO8095360.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            16853..17605
                     /gene="fnr"
                     /locus_tag="ACHK01_000357"
     CDS             16853..17605
                     /gene="fnr"
                     /locus_tag="ACHK01_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO8095361.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            17757..18707
                     /gene="uspE"
                     /locus_tag="ACHK01_000358"
     CDS             17757..18707
                     /gene="uspE"
                     /locus_tag="ACHK01_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO8095362.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(18757..19014)
                     /gene="ynaJ"
                     /locus_tag="ACHK01_000359"
     CDS             complement(18757..19014)
                     /gene="ynaJ"
                     /locus_tag="ACHK01_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO8095363.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            19258..20289
                     /gene="ynaI"
                     /locus_tag="ACHK01_000360"
     CDS             19258..20289
                     /gene="ynaI"
                     /locus_tag="ACHK01_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO8095364.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(20340..21953)
                     /gene="mppA"
                     /locus_tag="ACHK01_000361"
     CDS             complement(20340..21953)
                     /gene="mppA"
                     /locus_tag="ACHK01_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO8095365.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(22290..23189)
                     /gene="pgrR"
                     /locus_tag="ACHK01_000362"
     CDS             complement(22290..23189)
                     /gene="pgrR"
                     /locus_tag="ACHK01_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO8095366.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            23327..24247
                     /locus_tag="ACHK01_000363"
     CDS             23327..24247
                     /locus_tag="ACHK01_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8095367.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            24247..25179
                     /locus_tag="ACHK01_000364"
     CDS             24247..25179
                     /locus_tag="ACHK01_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8095368.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTFGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGVTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            25197..25835
                     /locus_tag="ACHK01_000365"
     CDS             25197..25835
                     /locus_tag="ACHK01_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8095369.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKSFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            26144..26872
                     /gene="mpaA"
                     /locus_tag="ACHK01_000366"
     CDS             26144..26872
                     /gene="mpaA"
                     /locus_tag="ACHK01_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO8095370.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(26847..27812)
                     /gene="ycjG"
                     /locus_tag="ACHK01_000367"
     CDS             complement(26847..27812)
                     /gene="ycjG"
                     /locus_tag="ACHK01_000367"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO8095371.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            27931..28437
                     /gene="tpx"
                     /locus_tag="ACHK01_000368"
     CDS             27931..28437
                     /gene="tpx"
                     /locus_tag="ACHK01_000368"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO8095372.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(28481..30022)
                     /gene="tyrR"
                     /locus_tag="ACHK01_000369"
     CDS             complement(28481..30022)
                     /gene="tyrR"
                     /locus_tag="ACHK01_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO8095373.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(30170..31231)
                     /gene="ycjF"
                     /locus_tag="ACHK01_000370"
     CDS             complement(30170..31231)
                     /gene="ycjF"
                     /locus_tag="ACHK01_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO8095374.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGTVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(31228..32625)
                     /gene="ycjX"
                     /locus_tag="ACHK01_000371"
     CDS             complement(31228..32625)
                     /gene="ycjX"
                     /locus_tag="ACHK01_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO8095375.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            32780..33778
                     /gene="ycjW"
                     /locus_tag="ACHK01_000372"
     CDS             32780..33778
                     /gene="ycjW"
                     /locus_tag="ACHK01_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8095376.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(33889..34794)
                     /gene="ompG"
                     /locus_tag="ACHK01_000373"
     CDS             complement(33889..34794)
                     /gene="ompG"
                     /locus_tag="ACHK01_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO8095377.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(34839..35921)
                     /locus_tag="ACHK01_000374"
     CDS             complement(34839..35921)
                     /locus_tag="ACHK01_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8095378.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(35935..36594)
                     /gene="pgmB"
                     /locus_tag="ACHK01_000375"
     CDS             complement(35935..36594)
                     /gene="pgmB"
                     /locus_tag="ACHK01_000375"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO8095379.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(36591..38858)
                     /gene="ycjT"
                     /locus_tag="ACHK01_000376"
     CDS             complement(36591..38858)
                     /gene="ycjT"
                     /locus_tag="ACHK01_000376"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO8095380.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(38855..39910)
                     /gene="ycjS"
                     /locus_tag="ACHK01_000377"
     CDS             complement(38855..39910)
                     /gene="ycjS"
                     /locus_tag="ACHK01_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8095381.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(39920..40708)
                     /gene="ycjR"
                     /locus_tag="ACHK01_000378"
     CDS             complement(39920..40708)
                     /gene="ycjR"
                     /locus_tag="ACHK01_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8095382.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(40726..41778)
                     /gene="ycjQ"
                     /locus_tag="ACHK01_000379"
     CDS             complement(40726..41778)
                     /gene="ycjQ"
                     /locus_tag="ACHK01_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO8095383.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(41809..42651)
                     /gene="ycjP"
                     /locus_tag="ACHK01_000380"
     CDS             complement(41809..42651)
                     /gene="ycjP"
                     /locus_tag="ACHK01_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8095384.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(42638..43519)
                     /gene="ycjO"
                     /locus_tag="ACHK01_000381"
     CDS             complement(42638..43519)
                     /gene="ycjO"
                     /locus_tag="ACHK01_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8095385.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(43540..44832)
                     /gene="ycjN"
                     /locus_tag="ACHK01_000382"
     CDS             complement(43540..44832)
                     /gene="ycjN"
                     /locus_tag="ACHK01_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8095386.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILLAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(44846..46525)
                     /gene="ycjM"
                     /locus_tag="ACHK01_000383"
     CDS             complement(44846..46525)
                     /gene="ycjM"
                     /locus_tag="ACHK01_000383"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO8095387.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFYPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(46738..47052)
                     /gene="pspE"
                     /locus_tag="ACHK01_000384"
     CDS             complement(46738..47052)
                     /gene="pspE"
                     /locus_tag="ACHK01_000384"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO8095388.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(47127..47348)
                     /gene="pspD"
                     /locus_tag="ACHK01_000385"
     CDS             complement(47127..47348)
                     /gene="pspD"
                     /locus_tag="ACHK01_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO8095389.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRSLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(47357..47716)
                     /gene="pspC"
                     /locus_tag="ACHK01_000386"
     CDS             complement(47357..47716)
                     /gene="pspC"
                     /locus_tag="ACHK01_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO8095390.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(47716..47940)
                     /gene="pspB"
                     /locus_tag="ACHK01_000387"
     CDS             complement(47716..47940)
                     /gene="pspB"
                     /locus_tag="ACHK01_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO8095391.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(47994..48662)
                     /gene="pspA"
                     /locus_tag="ACHK01_000388"
     CDS             complement(47994..48662)
                     /gene="pspA"
                     /locus_tag="ACHK01_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO8095392.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            48829..49806
                     /gene="pspF"
                     /locus_tag="ACHK01_000389"
     CDS             48829..49806
                     /gene="pspF"
                     /locus_tag="ACHK01_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO8095393.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(49926..51191)
                     /gene="puuE"
                     /locus_tag="ACHK01_000390"
     CDS             complement(49926..51191)
                     /gene="puuE"
                     /locus_tag="ACHK01_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO8095394.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(51229..52509)
                     /gene="puuB"
                     /locus_tag="ACHK01_000391"
     CDS             complement(51229..52509)
                     /gene="puuB"
                     /locus_tag="ACHK01_000391"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO8095395.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(52511..53998)
                     /gene="puuC"
                     /locus_tag="ACHK01_000392"
     CDS             complement(52511..53998)
                     /gene="puuC"
                     /locus_tag="ACHK01_000392"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO8095396.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(54273..54830)
                     /gene="puuR"
                     /locus_tag="ACHK01_000393"
     CDS             complement(54273..54830)
                     /gene="puuR"
                     /locus_tag="ACHK01_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO8095397.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(54857..55621)
                     /gene="puuD"
                     /locus_tag="ACHK01_000394"
     CDS             complement(54857..55621)
                     /gene="puuD"
                     /locus_tag="ACHK01_000394"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO8095398.1"
                     /translation="MENIMNNPVIGVVMCRNRLKDHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            55833..57251
                     /gene="puuA"
                     /locus_tag="ACHK01_000395"
     CDS             55833..57251
                     /gene="puuA"
                     /locus_tag="ACHK01_000395"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO8095399.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            57400..57564
                     /locus_tag="ACHK01_000396"
     CDS             57400..57564
                     /locus_tag="ACHK01_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095400.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            57554..58939
                     /gene="puuP"
                     /locus_tag="ACHK01_000397"
     CDS             57554..58939
                     /gene="puuP"
                     /locus_tag="ACHK01_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO8095401.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            59073..59318
                     /gene="ymjA"
                     /locus_tag="ACHK01_000398"
     CDS             59073..59318
                     /gene="ymjA"
                     /locus_tag="ACHK01_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO8095402.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            59631..61274
                     /gene="sapA"
                     /locus_tag="ACHK01_000399"
     CDS             59631..61274
                     /gene="sapA"
                     /locus_tag="ACHK01_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO8095403.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            61271..62236
                     /gene="sapB"
                     /locus_tag="ACHK01_000400"
     CDS             61271..62236
                     /gene="sapB"
                     /locus_tag="ACHK01_000400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO8095404.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            62223..63113
                     /gene="sapC"
                     /locus_tag="ACHK01_000401"
     CDS             62223..63113
                     /gene="sapC"
                     /locus_tag="ACHK01_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO8095405.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            63113..64105
                     /gene="sapD"
                     /locus_tag="ACHK01_000402"
     CDS             63113..64105
                     /gene="sapD"
                     /locus_tag="ACHK01_000402"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO8095406.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            64107..64913
                     /gene="sapF"
                     /locus_tag="ACHK01_000403"
     CDS             64107..64913
                     /gene="sapF"
                     /locus_tag="ACHK01_000403"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO8095407.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            64981..65334
                     /gene="ycjD"
                     /locus_tag="ACHK01_000404"
     CDS             64981..65334
                     /gene="ycjD"
                     /locus_tag="ACHK01_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO8095408.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQNWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            65702..66490
                     /gene="fabI"
                     /locus_tag="ACHK01_000405"
     CDS             65702..66490
                     /gene="fabI"
                     /locus_tag="ACHK01_000405"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO8095409.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            66635..67762
                     /gene="yciW"
                     /locus_tag="ACHK01_000406"
     CDS             66635..67762
                     /gene="yciW"
                     /locus_tag="ACHK01_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO8095410.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            67830..69764
                     /gene="rnb"
                     /locus_tag="ACHK01_000407"
     CDS             67830..69764
                     /gene="rnb"
                     /locus_tag="ACHK01_000407"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO8095411.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(69842..69946)
                     /locus_tag="ACHK01_000408"
     CDS             complement(69842..69946)
                     /locus_tag="ACHK01_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095412.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            70000..71985
                     /gene="pdeR"
                     /locus_tag="ACHK01_000409"
     CDS             70000..71985
                     /gene="pdeR"
                     /locus_tag="ACHK01_000409"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8095413.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(71967..72095)
                     /gene="ymiD"
                     /locus_tag="ACHK01_000410"
     CDS             complement(71967..72095)
                     /gene="ymiD"
                     /locus_tag="ACHK01_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO8095414.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            72132..72305
                     /gene="yciZ"
                     /locus_tag="ACHK01_000411"
     CDS             72132..72305
                     /gene="yciZ"
                     /locus_tag="ACHK01_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO8095415.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            72395..73144
                     /gene="yciT"
                     /locus_tag="ACHK01_000412"
     CDS             72395..73144
                     /gene="yciT"
                     /locus_tag="ACHK01_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO8095416.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            73413..73631
                     /gene="osmB"
                     /locus_tag="ACHK01_000413"
     CDS             73413..73631
                     /gene="osmB"
                     /locus_tag="ACHK01_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO8095417.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(73757..74083)
                     /gene="yciH"
                     /locus_tag="ACHK01_000414"
     CDS             complement(73757..74083)
                     /gene="yciH"
                     /locus_tag="ACHK01_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO8095418.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(74083..74820)
                     /gene="pyrF"
                     /locus_tag="ACHK01_000415"
     CDS             complement(74083..74820)
                     /gene="pyrF"
                     /locus_tag="ACHK01_000415"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO8095419.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CSLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(75013..76182)
                     /gene="lapB"
                     /locus_tag="ACHK01_000416"
     CDS             complement(75013..76182)
                     /gene="lapB"
                     /locus_tag="ACHK01_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO8095420.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(76189..76497)
                     /gene="lapA"
                     /locus_tag="ACHK01_000417"
     CDS             complement(76189..76497)
                     /gene="lapA"
                     /locus_tag="ACHK01_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO8095421.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(76646..77410)
                     /gene="pgpB"
                     /locus_tag="ACHK01_000418"
     CDS             complement(76646..77410)
                     /gene="pgpB"
                     /locus_tag="ACHK01_000418"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO8095422.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            77580..78170
                     /gene="ribA"
                     /locus_tag="ACHK01_000419"
     CDS             77580..78170
                     /gene="ribA"
                     /locus_tag="ACHK01_000419"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO8095423.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(78234..>80197)
                     /gene="acnA"
                     /locus_tag="ACHK01_000420"
     CDS             complement(78234..>80197)
                     /gene="acnA"
                     /locus_tag="ACHK01_000420"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO8095424.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKNRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        19867 a        20648 c        19796 g        19886 t
ORIGIN      
        1 ggacaacgaa aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaggggcc
       61 gttctacttc ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa acaaactttc
      121 tggtctggac aaggcgctgg ctgccggggc tgctgaaatc acgaaagaag aatatcttgc
      181 ccgcaaaaac ggaacataca cggacttacc gcaaaatgtg gataccgctg aagattccgt
      241 acaaccggag ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa
      301 tatcagccag cctgacgccg ataaattgct tgctgcatca cgtggtgaat ttgttgaagg
      361 gattagcgat ccgaatgatc cgaaatgggt gaaggggatt gaaacctgcg attctgtgaa
      421 ccagaaccag tcagaaacgg aacagaacga ccaaaaagcg gaacaaaaca gcccaaatgc
      481 gttacaagac gagccagaaa cgaaacaatc cgaaccagta gcgcaacagg aagaggaaaa
      541 agtctgtacc gcctgcggtc agagtggtgg cggcaactgc cctgattgtg gcgcggtgat
      601 gggcgacgca acataccagg aaacattcga tgaagagagt caggttgaag ctaaggaaaa
      661 tgatccggag gaaatggaag gcgctgaaca tccgcacaat gagaatgctg gcagcgatcc
      721 gcatcgcgat tgcagtgatg aaactggcga agtcgcagat cccgtaatcg tagaagacat
      781 agagccaggt atttattacg gaatttcgaa tgagaattac cacgcgggtc ccggtgtcag
      841 taagtctcag ctcgatgaca ttgctgatac tccggcactg tatttgtggc gtaaaaatgc
      901 ccccgtggac accacaaaga caaaaacgct cgatttagga accgctttcc actgccgggt
      961 acttgagccg gaagaattca gtaaccgctt tatcgtagca cctgaattta accgccggac
     1021 aaactccgga aaagaagaag agaaagcgtt tctgagggaa tgcgcaagca caggaaaaac
     1081 ggttatcact gccgaagaag gccggaaaat tgaactcatg tatcagagcg ttatggcttt
     1141 gccgctgggg caatggcttg ttgaaagcgc cggacacgct gaatcatcaa tttactggga
     1201 agatcctgaa acagcaattt tgtgtcggtg ccgtccggac aaaattatcc ctgaatttca
     1261 ctggatcatg gacgtgaaaa ctacggcgga tattcaacga ttcaaaaccg cttattacga
     1321 ctaccgctat cacgttcagg atgcattcta cagtgacggt tatgaagcac agtttggagt
     1381 gcagccaact ttcgtttttc tggttgccag cacaactatt gaatgcggac gttatccggt
     1441 tgaaattttc atgatgggcg aagaagcaaa actggcaggt cagctggaat atcaccgcaa
     1501 tctgcgaacc ctggctgact gcctcaatac cgatgaatgg ccagctatta agacgttatc
     1561 actgccccgc tgggctaagg aatatgcaaa tgactaagca accaccaatc gcaaaagccg
     1621 atctgcaaaa aactcaggga aaccgtgcac cagcagcaat taaaaataac gacgtgatta
     1681 gttttattaa ccagccatca atgaaagagc aactggcagc agctcttcca cgccatatga
     1741 cggctgaacg tatgatccgt atcgccacca cagaaattcg taaagttccg gcgttaggaa
     1801 actgtgacac tatgagtttt gtcagtgcaa tcgtacagtg ttcacagctc ggacttgagc
     1861 ccggtagcgc cctcggtcat gcatatttac tgccttttgg taataaaaac gaaaagagcg
     1921 gtaaaaaaaa cgttcagcta atcattggct atcgcggcat gattgatctg gctcgccgtt
     1981 caggtcaaat cgccagcttg tcagcccgtg ttgtccgtga aggtgacgag tttaatttcg
     2041 aatttggcct tgatgaaaag ttaatacacc gcccaggaga aaacgaagat gcccctgtta
     2101 cccacgtcta tgctgtcgca agactgaaag acggaggtac tcagtttgaa gttatgacgc
     2161 gcaaacagat tgagctggtg cgcagccaga gtaaagctgg taataacggg ccgtgggtaa
     2221 ctcactggga agaaatggca aagaaaacgg ctattcgtcg cctgttcaaa tatctgcccg
     2281 tatcaattga gatccagcgt gcagtatcaa tggatgaaaa ggaaccactg acaatcgatc
     2341 ctgcagattc ctctgtatta accggggaat acagtgtaat cgataattca gaggaataat
     2401 tcagcctggc ggtgtaatgc accgccaact tgaaatattt ttatgagaaa aattatgaga
     2461 tatgacaatg ttaaaccatg tccattttgt ggttgtccat cagtaacggt gaaagccatt
     2521 tcaggatatt accgagcgaa gtgtaacgga tgcgaatccc gaaccggtta tggtggaagt
     2581 gaaaaagaag cactcgaacg atgaaataaa cgaaccactg gaaataataa tggaggtgtt
     2641 catgtataaa attaccgcca ctattgaaaa ggaaggtggc actcctacta actggacaag
     2701 atattcaaaa tctaaactaa cgaaatcaga atgcgaaaaa atgctctcag gtaaaaaaga
     2761 agcaggcgtt tccagagagc agaaagtaaa actgataaat tttaattgcg agaaacttca
     2821 gtcctcgtga attgcattgt attcaaatta aaacttcata gctgattatt aataatcaac
     2881 atcgggcgtc aatttaagtc taacattggc gcctgccaga ggtgatgcga tggcacaagt
     2941 aatctttaat gaagagtgga tggttgaata cggcctgatg cttcgcactg gtctgggggc
     3001 cagacaaatt gaagcatacc gccagaactg ttgggtggaa ggcttccact tcaaacgagt
     3061 atctccttta gggaagccag acagtaagcg agggattatc tggtataact atccaaagat
     3121 aaatcagttt atcaaagact catgatatgt ctaaattacc aacaggtgtc gagattcgag
     3181 gtaaatacat tcgcatctgg ttcatgtttc gaggaaaacg atgtcgggaa acattgaaag
     3241 gctgggaggt tactaacagt aacattaaaa aagccgggaa tttaagagcg ttgatagttc
     3301 atgaaatcaa ttccggtgag tttgagtatt taagacgttt tccccagtcc agcactgggg
     3361 caaaaatggt gacaacgagg gtcataaaaa cgttcgggga gctttgtgat atctggacaa
     3421 aaattaaaga aacagagtta acaacaaaca caatgaagaa aacgaaatca caattaaaaa
     3481 cactcaggat aataatttgt gagagtactc cgatatcgca tattcgttat agcgatatct
     3541 taaactaccg gaatgaactg ctgcatggag aaacgcttta cctggataat ccaagatcca
     3601 acaaaaaagg aagaaccgtg cgcacagttg ataactatat cgccctgctc tgttcgttgt
     3661 tacgttttgc gtatcagtcg ggatttatat caaccaaacc atttgaagga gtaaagaaat
     3721 tacagagaaa cagaataaag cctgacccgt tatctaaaac agaattcaat gcattaatgg
     3781 aaagtgaaaa aggacagagc cagaacttgt ggaaatttgc cgtatactcc gggcttcgtc
     3841 acggggaact ggctgctctg gcgtgggagg atgtggattt cgagaaggga gttgtgaatg
     3901 tcagaagaaa cctgacgata ctggatatgt tcggtccccc aaaaacaaat gcggggatcc
     3961 ggacggtaac actactgcag cctgctcttg aagcactgaa ggagcaatac aaactgaccg
     4021 ggcatcatcg caaaagcgaa atcacctttt atcatcggga gtacggcaga accgaaaagc
     4081 aaaaactgca ttttgttttc atgcccaggg tgtgtaacgg aaaacaaaaa ccttattact
     4141 cggtaagcag tttgggggca aggtggaatg cagcagtaaa acgtgctggt attcgccgcc
     4201 gtaatccgta ccatacgcgg catacttttg cctgctggct gttgacggca ggagcgaacc
     4261 cggcatttat agccagccaa atggggcatg aaactgcgca gatggtgtat gaaatttacg
     4321 gtatgtggat tgatgacatg aacgacgaac aggtagccat gttgaatgcg cggttatcat
     4381 agttgcaaag tttgccccca atttgcccca tttagtacca gagaactgaa ataatgcaag
     4441 aaaatcaaca aattacaaag aaagaacaat acaacctgaa caaattacaa aaacgtctgc
     4501 gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc gatcgcatca
     4561 tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg cgcaatttgc
     4621 agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa aagcaaccgg
     4681 gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag tacaagattg
     4741 ttgaagagaa tacttacggt atcgtgaaag agaagattcc agagggcaaa accacttgct
     4801 cactgtgttc tcgccttcgt cgcggtatcc tttatcgtac cgcaacggaa ctgggggcga
     4861 cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc ttaaatatgt
     4921 tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat ggcaaacata
     4981 tcgttattcg tccgctggcc tactgccgcg agaaagatat tcagcgattt gccgatgcaa
     5041 aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg caacgtcagg
     5101 tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc gagacgatgt
     5161 tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg ttcgatttta
     5221 aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt gatcgcgaag
     5281 agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag ttggatgagt
     5341 tacgactgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc aagcgtatcg
     5401 ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca atcccgaata
     5461 agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac cgcacccggc
     5521 acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga gcaacagcct
     5581 gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata tctgcgccat
     5641 caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt ttggcttttt
     5701 tcccgccatc gatacacaac gttgccattt ctgcttccag cggcacaatg gaactattag
     5761 ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata ttggcccgct
     5821 gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca cgagctgtac
     5881 gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc accaccagct
     5941 caagcgattt aatatccaga ccacgcgcag caacatcagt agcaaccagt acacgggcgc
     6001 taccgttagc aaaacgtacc agagtctgat cgcgatcgcg ttgctccaga tcgccgtgta
     6061 acgacaatgc actttgccct acttcattca gcgcgtcgca gacagcctgg caatcttttt
     6121 tggtattgca aaacaccacg caagaggatg gctgatgcaa gcttaataac cgttgcaaca
     6181 gaggaatttt gcctttgctg gatgtctcat aaaattgttg ttcaatgggt ggcaaagcat
     6241 ctgttgagtc aatttcaatc gccaaaggat cgcgttgtac tcgtccgctg attgcggcga
     6301 tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc gcaaaacgga
     6361 tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc tcatccatca
     6421 ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc agcaaacgac
     6481 ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc ataccgaacg
     6541 gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca
     6601 attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa gcctgggttt
     6661 gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc
     6721 tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga agcgcggcgg
     6781 cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt tgggcgggag
     6841 gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat cgacgcaaag
     6901 ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggttcttc
     6961 atcaggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca ctggaacagg
     7021 atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc
     7081 ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg caaaaaaaag
     7141 ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg
     7201 aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagggag
     7261 atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg gcgtttttgt
     7321 atacagacag ccggaaaaat tgcttttgtt acaaccattt actacgatgc aaccataaag
     7381 caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccacc
     7441 cgccgccagg gatcccacca aggttgacgc caaataaccc tgtcagaaag gtactgggta
     7501 aaaagaccat tgccatcaat gacattgtat aggtacgacg agctaaattt tcctgcatca
     7561 cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa
     7621 gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca tcgctcatcc
     7681 acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac
     7741 gcatcacgat taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat
     7801 caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata aattcgctgg
     7861 aatgatcggt caacgcatcg cacacatcca ccagccaccc tccgcaatcg gtcggacccg
     7921 tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt tgtcgggtcg
     7981 aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat
     8041 cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaga cggctgactc
     8101 ggggccgagt gctctcgcct gccagcgcat cacgtacgtt attgggaagc agcggtgttg
     8161 tcgccagcca ttgggcgctt tcatggtgta cataattaag gtggagccag cagggatgcg
     8221 cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc
     8281 aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc acagtgcctc
     8341 catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca
     8401 ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg
     8461 cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct
     8521 gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa aaataacggg
     8581 atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatt
     8641 gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact gttgatgcaa
     8701 gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca aaaagaactg
     8761 ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct cactgttcag
     8821 cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct gacagaaaca
     8881 gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt aaaagccagt
     8941 cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac
     9001 tctgcaccaa tgttgttgat tgacccctca cgagatggcc aaatcgtcga tgctaacctc
     9061 gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca tacctgggaa
     9121 attaatatgc tcgggcgtcg cgtcatgcct atcatgcatg aaatttcgca tttacccggc
     9181 ggtcataaac ctttgaattt tgttcataaa ctggcggatg gatctactcg tcatgtgcag
     9241 acctatgccg ggccgattga aatttatggc gacaagctca tgttatgtat tgtgcatgat
     9301 attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca tgacgcgatg
     9361 accggattac tgaatcggcg acagttttat cacattaccg agccaggcca aatgcagcat
     9421 ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt
     9481 aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc ccgcaccctt
     9541 gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta
     9601 ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgtgtaagc
     9661 gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg tgtggcgcat
     9721 cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcgg
     9781 gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc gtctcgagat
     9841 caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc cgttttacgt
     9901 agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcagtgca atatgcctga
     9961 acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgcgat
    10021 ttatcatccc gtcctttgcc atgaataatc agcacgttac gcaaaccatc agccatggct
    10081 tgttgaatga aactgaacat cattttgcgg cactcttcca ccggctggcg caaaagattc
    10141 aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc atgttgcaac
    10201 ccttcccgcc gaaactccag cggctgactt agtgggatga tgtcgagaaa tccggtggtg
    10261 aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga
    10321 tgccagtgga catcggtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac
    10381 agcgatttgt cgtcaaggtt catgttacat cctcccgcaa ttaagagcgc gatatgataa
    10441 ccagaccggg tcggtccaac aacgtattac ccaaaattcc agtaataagt tccaaatatt
    10501 gccgatattt taagcaaaat gcttatgcat gattattcat tcacgatatt aataatgtaa
    10561 cttatatttt cgtgaaatct gtcactgaag aaaattggca actaaaggtt aaaaccgtta
    10621 taacacagtc accggcgcag aggagacaat gccggactta agacgcggat gcactgctgt
    10681 gtgtactgta gagtctggcg gatgtcgaca gactctattt ttttatgcag ttttaacttt
    10741 gcagatagcc gcattctcgc cggaattgcg tgattaatga cgcggtcagt ggtgtctggc
    10801 gactatcacg ccgctgaatc aaataatagg ccgctttcgg taaaatttcg ctaaccggca
    10861 acatcaccag cccctgtccg tgcaagggat cgcagcccat ttcttcaggc agtatgctga
    10921 gaaaatcact ttttgccacc agactgatac aggctgagaa cgtctcgcag actacaccga
    10981 cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa ctgtttgtag taactgccgt
    11041 gtggcgtcgg cattgtccag ctgtaatcca gtaactgttt gatcgaacgg gcaccaatgg
    11101 cggggtgtcc cgggcggcaa aagatcgcga attgcttttc cagtaatttc tcaaaagtaa
    11161 attcgtggtc gtacggtccc tgataatagg tattgatggt gaaatccaat tctccctgac
    11221 gcaattcatt aatcatcgac accagttgcc cttccataat gcgtactttt acctgcggat
    11281 gctgctgatg aaaacgagat atgacagctg gcatcagact gcgggaaata ctggccccca
    11341 tgccgatatt aatctgccct gccagttgcc cttgtcgttg gcgaatttcc tcttgcgctg
    11401 cgcgcagctc ttcaagaata agactggcgt gctgataaaa actttcaccg gcatcagtta
    11461 acgtcacgcc tttactacgg cgaaaaaaga gttgcgccgc taatccttct tccagctcct
    11521 gaatagattt actcagtgcc ggttgcgaca tattcaacat tcggctcgct ccgcgaatgc
    11581 tgccctgacg agccacttca acaaaagcac gaatttgatg aatttttacc tgaaaagcca
    11641 tgacgccacc gataaccgtt atttatcaga ccaaagaaac tggcatctac tttaatgcag
    11701 atgattgtgt cagggtaatt tatgaacggt taaaactgtg aaaaatcagt tagtgataag
    11761 taaaaactat cgctacatga accgggtcac actttttact gatgacggga aaggttatgg
    11821 agtctttgaa tcaatttgtt aattcgcttg ccccaaaatt atcgcactgg cgacgtgatt
    11881 ttcatcacta tgcagagtct ggctgggtgg aattccgcac tgccaccctt gttgcggaag
    11941 aattgcagca gctcggctat tcactggcgc tgggccgcga agtagttaat gaaagtagcc
    12001 ggatgggact acctgatgaa ctcactctac aacgcgaatt cgagcgcgct cgtcaacagg
    12061 gtgcgctagc acaatggatt gcggtttttg aaggtggttt cactggtatc gtcgccaccc
    12121 tggataccgg tcgccccggt ccggtgatgg ctttccgtgt cgatatggac gcgctggatc
    12181 tcagtgaaga gcaggatgtc agccatcgcc cctaccgcga cggttttgcg tcatgtaacg
    12241 ccggaatgat gcatgcctgt ggtcatgatg gacataccgc cattgggctt gggatggcgc
    12301 atacccttaa acaattcgag tccggactac atggcgtcat caaactgatt tttcagcctg
    12361 cagaggaagg tacgcgtggc gcgcgggcga tggtcgatgc aggtgtcgta gatgatgttg
    12421 attattttac tgccgtgcac attggcactg gcgtacctgc gggcaccgtg gtgtgcggca
    12481 gtgataattt tatggcaacc accaaatttg acgcgcactt caccgggacc gccgctcacg
    12541 caggcgcaaa accagaagac ggtcacaatg ccttgttggc ggcagcacaa gcaactcttg
    12601 cactgcatgc aatcgccccg cacagcgaag gagcttccag agtaaacgtg ggcgttatgc
    12661 aggcaggaag cggccgtaac gttgttcctg cctcggcgtt gctgaaagtg gaaacacgcg
    12721 gggccagcga cgtcattaat caatatgttt ttgaccgtgc acaacaagcg attcagggcg
    12781 cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat gggtgcagct accgccagtt
    12841 ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc ggctcaggtc gcgggggtca
    12901 atcaggccat tgaacgtgtt gaagcgcctg cgggttccga agatgccaca ttaatgatgg
    12961 cccgcgtgca gcaacatcaa gggcaagcct cctacgtggt gtttggcaca cagctggcgg
    13021 caggtcatca caacgaaaaa ttcgattttg acgagcaggt tctcgctatt gccgtcgaaa
    13081 cgctggcgcg caccgcgctc aattttccct ggacgcgagg tatctgatgc aggaaatcta
    13141 tcgttttatc gacgatgcga ttgaagccga tcgccaacgt tataccgata ttgccgatca
    13201 aatctgggat catccagaaa cacgttttga agagttctgg tcagcggagc atctggcttc
    13261 ggcgctggaa tctgcaagct tcaccgttac ccgcaacgta ggcaatatcc caaatgcatt
    13321 tattgcttcg tttggtcaag gcaaaccggt tatcgccctg ctgggagaat atgacgccct
    13381 ggcaggttta agtcagcaag ctggttgcgc gcaacctaca tccgcgacgc ccggtgaaaa
    13441 tggtcacggt tgcggacaca atttgctggg aaccgccgcc tttgccgctg caatagccgt
    13501 caagaaatgg ctggaacaat atgggcaagg cggcacagtg cgcttttatg gttgtcctgg
    13561 cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag ggggtatttg atgatgtgga
    13621 tgcggcactc acctggcacc cggaagcctt tgccggcatg ttcaataccc gcacgctggc
    13681 aaacattcag gcatcatggt gctttaaagg gatcgcagca catgccgcga attcccctca
    13741 tttgggacgc agcgcccttg atgccgtaac gttgatgacc actggcacca acttcctcaa
    13801 cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc acaaatagcg gcgggatctc
    13861 gcccaacgtg gtccaggcgc aggcagaagt gctttatctt atccgtgccc ccgaaatgac
    13921 cgacgtgcag catatttatg atcgggtcgc caaaatcgcc gaaggtgcgg cattgatgac
    13981 cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc agttatctcc cgaatcgcac
    14041 cttagaaaat gccatgtacc atgccctatc ccattttggt accccggaat ggaactccga
    14101 agaactggct tttgcgaaac aaattcaggc tacgctcacc cccaacgatc ggcaaaacag
    14161 tctgaataat atcgccgcaa ccggtggcga aaacggcaag gtttttgcac tacgtcatcg
    14221 tgaaacggta ctggcgaatg aggtcgctcc atatgccgcc accgataacg tgcttgcggc
    14281 atcgactgat gtcggcgacg tcagttggaa actgcctgtt gcccagtgtt tcagcccctg
    14341 ttttgccgtc ggtacaccgc tacatacgtg gcaactggtt agccaggggc gaacatctat
    14401 tgctcataaa ggaatgctgc tggcggcgaa aactatggca gcaaccacag tcaatctctt
    14461 cattgattca gggctattgc aagaatgcca acaagagcat cagcaagtta cggacacgca
    14521 accgtatcac tgccctatcc cgaaaaacgt gacaccgtca cctttaaaat aacaacaacg
    14581 caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt cctcccaatc
    14641 cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttccccatc cttttttgct
    14701 ctttatctat ttgataatcg tactcatggt gacgacggca attttgtcag cctttggcgt
    14761 cagtgcgaaa aacccgaccg atggtacgcc ggtggtggtg aaaaacctgc tcagtgtgga
    14821 aggattacac tggtttttac ccaatgtgat taaaaacttt agcggttttg ctccacttgg
    14881 agcgatcctg gcgctggttt taggtgccgg cctagcggag cgcgtcggct tactgccagc
    14941 gctaatggtt aaaatggcat cgcatgttaa tgcccgctac gccagttata tggtgctgtt
    15001 tattgctttt ttcagccaca tttcttccga tgcggcgtta gtgatcatgc caccgatggg
    15061 tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg ctgccattgc
    15121 aggcgtaggt tgcggcttta cggctaattt actgattgtc acaaccgacg tgttgctgtc
    15181 ggggatcagc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca gtgtaattga
    15241 taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg gcttgataac
    15301 cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg agaaactgca
    15361 gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat cgctactttt
    15421 tattgctgcg atcgcgctga tggtgatccc ggaaaacggg atattgcgcg atccgattaa
    15481 tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactaatca ttcttttttt
    15541 ctttgtggtt tcgctggctt atggcatcgc tacccgcaca attcgacgtc aggcggattt
    15601 accgcattta atgattgaac cgatgaaaga gatggcggga tttatcgtga tggtttttcc
    15661 cctcgcccag tttgtcgcca tgtttaactg gagcaacatg gggaaattca tcgccgtggg
    15721 gctgaccgat atacttgaaa gttcagggct tagcggcatc ccggcgtttg tcggtctggc
    15781 gttgctttcc tctttcttat gcatgtttat tgccagcggt tccgcaatct ggtcgattct
    15841 ggcccccatt ttcgtaccaa tgtttatgct acttggcttt cacccggcat ttgcgcaaat
    15901 cctctttcgt attgccgact catccgtatt gcctttagcg ccggtatctc cttttgttcc
    15961 actgtttctt ggattcctgc aacgctacaa accagacgcg aaactgggta cttactattc
    16021 gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgctgatgt tgctggcgtg
    16081 gtatcttgtt ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac
    16141 ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccactg tgggtgattt
    16201 gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc
    16261 agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc accaacccag
    16321 gcggtttaag cgacaagctt cgtgattatt ttgccggtaa tcttagcatt attgatacgc
    16381 ttcccactgc cacagggggg acgccatttc agcgcgaagt ctggaaaact ctacgcacta
    16441 tcccctgcgg gcaggtaatg cattacggcc aactggctga acaattgggc cgccctggcg
    16501 cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat cagcatcgtc gtaccttgcc
    16561 atcgggttat tggccgaaac ggcaccatga ccggatatgc aggcggagtt cagcgaaaag
    16621 agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc
    16681 ttgttcacac ttttatgtaa agttaccctt aacaacttaa gggttttcaa atagatagac
    16741 atatatttac atctaatatc ggaattctct gctgttaagg tttgcttaga cttacttgct
    16801 ccctaaaaag atgttaaaat tgacaaatat caattacggc ttgagcagac ctatgatccc
    16861 ggaaaagcga attatacggc gcattcagtc tggcggttgt gctatccatt gccaggattg
    16921 cagcatcagc cagctttgca tcccgttcac actcaacgaa catgagcttg atcagcttga
    16981 taatatcatt gagcggaaga agcctattca gaaaggccag acgctgttta aggctggtga
    17041 tgaacttaaa tcgctttatg ccatccgctc cggtacgatt aaaagttata ccatcactga
    17101 gcaaggcgac gagcaaatca ctggtttcca tttagcaggc gacctggtgg gatttgacgc
    17161 catcggcagc ggccatcacc cgagcttcgc gcaggcgctg gaaacctcga tggtatgtga
    17221 aatcccgttc gaaacgctgg acgatttgtc tggtaaaatg ccgaatctgc gtcagcagat
    17281 gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac atgatcctgc tgttgtcgaa
    17341 gaaaaatgcc gaggaacgtc tggctgcatt catctacaac ctgtcccgtc gttttgccca
    17401 acgcggcttc tcccctcgtg aattccgcct gacgatgact cgtggcgata tcggtaacta
    17461 tctgggcctg acggtagaaa ccatcagccg tctgctgggt cgcttccaga aaagcggtat
    17521 gctggcagtc aaaggtaaat acatcaccat cgaaaataac gatgcgctgg cccagcttgc
    17581 tggtcatacg cgtaacgttg cctgattttt ccgcataact cactatcctt ctgtcatatc
    17641 attaaatttt tctgatttat tgatctggca gaaggttcat cactgtttca ttcaccagat
    17701 atgggttaat cttttaatta caaactgcgt tgacagttgt tgtaaggaga ccctgtatgg
    17761 ctatgtatca gaacatgctc gttgttatcg atcctaacca ggacgaccaa ccagcattgc
    17821 ggcgagctgt ttatttacat caacggattg gtggcaaaat taaagccttt ttgccgatct
    17881 atgacttctc atacgaaatg accaccctgc tctccccgga cgaacgtacc gctatgcgtc
    17941 agggcgtcat cagccagcgt acagcctgga tccacgagca ggcaaaatat tatctcaatg
    18001 ctggcgttcc cattgaaatt aaagtggtct ggcataaccg tcccttcgaa gccatcattc
    18061 aggaagtgat cagcggcggg cacgatttgg tgctaaaaat ggcgcaccaa catgaccgtc
    18121 tggaagcggt gatttttacg ccaacggact ggcatctgtt acgcaaatgc ccaagcccgg
    18181 tgtggatggt gaaagaccag ccgtggccgg aaggaggcaa ggcgctggtg gcggtgaatc
    18241 tcgccagtga agagccgtat cataatgcgc tcaatgaaaa actggtcaaa gagacgatcg
    18301 aactggcaga acaagtcaac cataccgaag ttcatctggt tggcgcttat ccggtaacgc
    18361 caattaatat cgcgattgaa ctgccggaat ttgatccgag cgtttataac gatgccattc
    18421 gtgggcaaca tttgctggca atgaaagctc tgcggcagaa attcggcatt aatgaaaaca
    18481 tgacgcacgt agaaaaaggt ctgccagaag aggtgattcc tgatttggcg gagcacttac
    18541 aggcggggat tgtggttctg ggcacggtag gacgcaccgg tatttcagca gcattcctcg
    18601 gcaacacggc ggaacaggtg attgatcatc ttcgctgcga tctgttggtc attaaacctg
    18661 accagtatca gacacccgtt gaactggatg acgaagaaga cgattaacga ctcttcaata
    18721 actgacaagg cccgagttat cgggcctatt ttttacttac tgctcttcgc cgccaaggaa
    18781 ataaattccc aacgggattg ccagcaacac agtaaagacc aggctataaa caaaaatcat
    18841 cgatgactga atcacataca ttgatgtcgt ccactcagac agcggaatat tgtactgttc
    18901 tataacgccg ccgatagtcg cgcgagtcac aaccgacgcc gcaataatga aaaccgccaa
    18961 caaaccaaag agaaatttct tccctttcgc tgacttcagt ttcgccataa tcatgatgta
    19021 atccttgtgc agacaaattt cttgttacct aaacgtaaca ataccatgac atttcaccgt
    19081 tgtctgctct gaaaacctca cctcttagct ggatttacaa tcaacgcatt gattagttag
    19141 ctaatgttgc tacttccttc cctgactatt gatgatctga atgaattgtc gcggtaaaat
    19201 agccccgcca ggggtttccg gcatgacgac aattatctgg aggagcctga taaaacaatg
    19261 atcgctgaac tgtttacaaa taatgcgctt aatctggtca ttattttcgg tagctgcgca
    19321 gcattgattc tgatgagctt ttggtttcgc cgcggaaatc gtaaaagaaa aggtttttta
    19381 ttccatgcgg tgcaattttt aatctacacc ataattatca gtgctgttgg tagcattatt
    19441 aattatgtta tagaaaacta taaactcaaa tttatcaccc caggcgttat cgattttatc
    19501 tgtacgtccc tgattgcggt tattctgacg attaagctat ttttgctgat taatcagttt
    19561 gaaaaacagc agattaaaaa aggtcgcgat atcaccagtg cgcggattat gtcgcgcatt
    19621 atcaaaatca ccattattgt ggtgcttgtt ctgctttatg gcgaacattt cggcatgagc
    19681 ctttctggct tgctgacctt tggtggtatt ggtggtctgg ctgtcggtat ggccggtaaa
    19741 gatattctga gtaatttctt ttccgggatc atgctctatt tcgaccgtcc tttcagtatt
    19801 ggcgactgga tccgttcacc ggacagaaat atcgaaggta cagtagcgga aattggctgg
    19861 cgaattacca aaattacgac ctttgataat cgtccattgt acgtaccgaa ctcgctgttt
    19921 tcgtcgatca gcgtagaaaa cccaggacga atgaccaacc gccgcattac cacgaccatt
    19981 ggtttacgtt atgaggatgc ggcaaaagtg ggcgttattg tcgaagccgt acgtgagatg
    20041 ctgaaaaatc acccggccat cgaccagcga caaaccttac tggtttattt caaccagttt
    20101 gccgactcgt cattgaatat tatggtttat tgctttacca aaaccacggt atgggctgaa
    20161 tggcttgctg cacagcaaga cgtttatttg aagattatcg atattgtgca gtcacacggc
    20221 gcggactttg ccttccccag ccagacgctg tatatggata acattacacc gcaggaacag
    20281 gggcgctaaa tcagacatca ctggcgttat ccacaagggt aacgccagtt cccatcacat
    20341 caatgcttca caatatacat agtccggctg tacgccacat cttccgggtt attaatggga
    20401 taacctttca gccacggctt gattaatcgt ccattggtat attgataaat tggtgcaatc
    20461 ggtgcttgct ccatgaggat tttttctgcc gcgttgtaat cggcattacg cgctttaacg
    20521 gtattttccg tcgatgcctg ggccagaact ttgtcgtatg ccggattgtt aaagcgtgaa
    20581 atatttcctg aatgcgttga cgttaataat gtcaggaaag tggagggttc attataatcc
    20641 cccacccatg aggcgcggat aacatcaaaa ttgccggtgt tacggctatc gatataggtt
    20701 ttccattcct gattttgcaa tttaacatct acgccaaggt tctttttcca catcgatgct
    20761 acagcaatcg caattttttg atggttttct gaagtgttat acaaaagcgt cagcttcagc
    20821 ggtttttgcg gaccataacc agctgcgctc aacaaagttt ttgcctgcgc attcagttct
    20881 tcctgactca tttgttcaaa cggcgaaggt tccggcgtaa atccggcggt aacatctggt
    20941 gtaaaatgcc acgctggctt ttcgcccgtc cctaatactt tttcggtcat caggcggcga
    21001 tctatcgtca tacttaatgc cagacgaacg cgctgatctg ccgtcggccc tttttgcgtg
    21061 ttaaacgcat aataataggt cccgagctgc ggcggcgtat aaacctgccc cggaatatcc
    21121 ttcaacagct tctgatacat atttttcggg aaggattcgg tgatatcaat atcccccgcg
    21181 aggtaacgct tagtggctgc ggattcctga ttaattggca ggaaggtcac tttttgcagt
    21241 accgttttgg cgttatccca ataatgggta ttcggtacca cgaccagttt ttcattgact
    21301 acgcgttctt taagaacata agcgccattg ccgatcagat ttccgggttt cgtccactct
    21361 ttaccgcttt ctacgttggc tttttgcacc gggaagaagg caaagttagc ggttaaattc
    21421 acaaaccacg gcaacggttt atcaagctga attttcaagg tatgggcatc aactgcggtg
    21481 acgccaagct ggtcaggcgt agctttacca tcaataattg cctgtgcgtt gttgattccc
    21541 gccagcgcgg caaaccatgc aaatggcgat agcgttttcg ggtccaccag acgttgccag
    21601 ctgtagacaa aatcttgcgc cgttaccggt gtgccatccg cccattttgc gttatcgcgc
    21661 agggtaaaag tccagatacg gttgtcatta cttttccact gagtcgcaac gccggggaca
    21721 atctcccctt tctcgttctg attcaccagt ccttcaaaca gatcgcgaat gacctgaatc
    21781 tctggcaggc ccacggcttt agcgggatcc agcgacgcag gctcatcttt aatgtggcgc
    21841 accagctcct gcttctctgc cagtactgtg ccgctcggaa cttctgcagc atacgaaaga
    21901 gaaatgctgc tgaccaacag cgcacaacac gtgactgaaa cagagtgctt cataagtttc
    21961 cctctaaccg acattaaaat tgatgcgtaa ttatttgttt cgccttaaag aaatgcaaac
    22021 aacttccatc aaaaaagtga cacaggaagc ataaaatgtt ttatgtatca atatattaaa
    22081 tcagatttag tgtaaataat cgacgccaaa atgcggcaaa gcacatagaa caccgaacac
    22141 tacgttcagg cttcgcacgt tcaaaccagt tttcggtatc aaaatcagca tctgtcgcca
    22201 ttgtacgaag aataaaaaat aaccaaaacg ttgaaaggaa ctgagaatac gttaagagag
    22261 aaattaaatt aatgtatcag caagtagtgc taaacagcac ctttatactt cagcctatct
    22321 ataaataatg agaatgccga gccagcatgt cgacggtgtg gatagtacag gtgataaccg
    22381 ggtaaatctg gtgtgaattt atccaaaaca cgtatcagtt ttttttcttt aatagcccgc
    22441 tcaacctgat cataaggtag atacgccaat ccatgcccat caattgcagc atcaatgatc
    22501 aggtctatcg tattcagtaa aagctgacct tccatgcgaa cacgaacttc acgtccaccg
    22561 cgtattaatc tccagcgatt tgctgtaccc gatgtgggaa gatacaaatt aattgcctga
    22621 tgatctatta attgtgacac tgacgttgga acacttcggc gagaaaaata atccggtgag
    22681 ccaacaatag ccattggtat atctggcccg attcgaatgg cgatcatatc tttatccatc
    22741 tccccaccca gacggacgcc tgcatcaaaa cgttcagaaa cgacatcggt caaaccataa
    22801 tcaatggtga gctgaatatc aatttcagga tgcgatttca ggaatgtgcg cattgctggt
    22861 aacaatatcg tttttgctgc atgttctaca gtagtaatac gtattgtccc ggatgggcgg
    22921 ttctgcagat cgctcaggga tgccatggct gaatctatgt catgcaacat agggccaaga
    22981 acagacaaaa gatgctcgcc cgcttcagtt ggaacaacgc tgcgcgtggt tcgcgtcaga
    23041 agccgcaatc ccaatcgttc ttctatacga cgcactatct ggcttaaagc tgactgcgcc
    23101 atgctcaggc gggctgctgc acgagtgaag ctacgctcct ctgcaacgac gacaaacgcc
    23161 atcagatcag cgatttcttc acgcttcatt cagtatctcc atgcaatcta ttaatcattt
    23221 aaatgatagg tctaatcatg attttgggtc taatcaactt aatgatcaat atctatagtg
    23281 atatcaccaa ccgttaaaac gaaccgacaa ctccatgagg aacggaatga tgaataataa
    23341 agtcagcttc actaacagca ataatccaac catctctttg tctgcagtga tctatttccc
    23401 tccaaaattt gatgaaaccc gtcagtatca ggcaattgtg ttatctcacc caggtggggg
    23461 cgttaaagaa caaacggccg gaacctatgc caaaaaactg gcggaaaagg gatttgtcac
    23521 tattgcttat gacgcatctt atcaaggtga aagtggcggc gagccgcgtc agttagaaaa
    23581 cccatacatt cgtactgaag acatcagtgc cgtgattgat tacctgacaa cgctttctta
    23641 tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc ggtgcaggat atacagccaa
    23701 tgctgcaatt caggatcgcc gcatcaaggc catcggtacc gttagtgcgg tcaatattgg
    23761 ttcgatgttc cgtaatggtt gggaaaacaa tgtgaaatct attgatgccc tgccatacgt
    23821 tgaagcgggt tcaaacgccc gaaccagcga tattagcagc ggagaatatg ccgttatgcc
    23881 actggcacca atgaaggagt ccgatgcgcc gaacgaagaa ctgcgtcagg cctgggagta
    23941 ttaccacacc cctcgcgcgc agtatccaac agcaccgggt tacgctactt tgcgcagcct
    24001 taaccagatt attacctatg atgcttacca tatggcggaa gtgtacctga ctcagccgat
    24061 gcagattgtg gcgggcagcc aggcagggag caaatggatg agtgacgacc tgtacgatcg
    24121 ggcatcaagc caggataaac gctatcacat tgttgaaggc gcaaaccata tggatcttta
    24181 tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta gctccgttct ttgaggaaac
    24241 tctgtaatga aacatattca aatccgcaat agtgatatgg cctggcatat tgctgccaat
    24301 attcagtttc caccaaattt tgatgagtca aaacagtatc cggcgattat cagcgtccat
    24361 ccttttggca gttgtaagga gcagacgttt ggcaatatct atggcaaagc attggctgaa
    24421 aaaggctatc tcgttctcgc ttatgacgcc agtttccagg gagaatctgg tggggagcct
    24481 cgctggatag aagatccgac acagcgcgta gaggatatta gccgtgttat cgattatgca
    24541 gtaactctgc catatgttaa tgctgaacgt attggcgtgc tgggagtgtg tgggggtggt
    24601 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagcagtagt gagtattacg
    24661 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attacgatcc gattggcgca
    24721 ctgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
    24781 gaattacttc ccgccagcct ggatgcagca aaagcccatg gagtgaccga acgcgatgtt
    24841 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
    24901 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
    24961 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
    25021 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
    25081 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
    25141 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
    25201 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
    25261 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
    25321 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
    25381 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
    25441 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
    25501 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
    25561 ttggtgagcc cctaaaatca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
    25621 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
    25681 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
    25741 tcacagatat catcgataaa cctgagaaac acattggtga taatataggt atcaatcaac
    25801 ccgtgactga tggtgacaag ccattattca tgtaaatcaa ttacatagtt accatcgtaa
    25861 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
    25921 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
    25981 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
    26041 aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat
    26101 ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg taaaccgccc
    26161 acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg
    26221 cgcgccgtta atctggtttc ctgcccctgc agccagtcgc gaaagtggtt tgattctggc
    26281 tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc tgcggacttt
    26341 gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagct
    26401 ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaactg
    26461 gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt
    26521 actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc agcttataca
    26581 ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta ttgaagatcc
    26641 cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag
    26701 cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca acttgcattg
    26761 catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc
    26821 gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc
    26881 gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc
    26941 ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc
    27001 agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg
    27061 gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta
    27121 cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc
    27181 gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc cgccaatagc
    27241 tggcaacgcg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac gatcagcgtc
    27301 gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg gttatccagc
    27361 ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga
    27421 tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag cgttatgccg
    27481 atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa tgcacaatcc
    27541 agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct
    27601 ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc actttctcca
    27661 taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc
    27721 accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc
    27781 caggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg cggtggtctg
    27841 cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac
    27901 gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac
    27961 ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc
    28021 gtagcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg
    28081 aacattttcc cgagtattga taccggagtt tgcgccgcat cagtacgtaa gtttaaccaa
    28141 ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct gccgttcgcc
    28201 cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt
    28261 aacgctgaat tcctgcaagc ctacggtgtg gcaattgctg atggcccact gaaaggtctg
    28321 gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg
    28381 gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataattt
    28441 aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct
    28501 gactcagacc atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt
    28561 ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa
    28621 aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg
    28681 cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg
    28741 tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac gcataacgcg
    28801 taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa
    28861 agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca cgtagcggcg
    28921 gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac atgccttttt
    28981 gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct
    29041 catggtcttc gccaacccga cggaaagtgc catcattaag gaaacgcagt aatttcgcct
    29101 gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg ttcgcctgct
    29161 caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt
    29221 ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat
    29281 ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg
    29341 cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg
    29401 cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca
    29461 tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat
    29521 cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct
    29581 cgttatggga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca
    29641 attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cgcaaaaagc tgacagctcg
    29701 ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt tcgggcaacg
    29761 cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac
    29821 gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa
    29881 actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta
    29941 aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt
    30001 cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg
    30061 atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga
    30121 aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc
    30181 tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga cgacggaaat
    30241 ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt
    30301 taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca
    30361 aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg
    30421 ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc
    30481 ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg cgccaggcga
    30541 taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg
    30601 ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat
    30661 acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac cagcgttgca
    30721 gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcggc
    30781 ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct
    30841 gtcgcaagcg ccataagcgc cgccactctg ttaccacagt acctacgcca gcgccgataa
    30901 tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc caggcattca
    30961 ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca
    31021 ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt
    31081 gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt
    31141 cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc ggaccgtcga
    31201 aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattcaag
    31261 cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa
    31321 cgcttcaaac tggaaccctt gcttgtccca gaacgcctga ccgggcaaac gcgcaggaac
    31381 ttcaccggga taaacggtga gtggcgcacc atcgctgagc cggttaccgc gcaatgccgg
    31441 aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc
    31501 caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct gaatcagttg
    31561 ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg ctttagtggc
    31621 agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg tacgctgccc
    31681 gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg
    31741 cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa agcgcagaaa
    31801 atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc gctcgcgcag
    31861 cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc aggcatcgac
    31921 atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcccg gcaagacaaa
    31981 gcgcccaggc tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca
    32041 cgcggcggca atgtctgcca gccggttttc gtcggcaggc gctagcgggt ccagcccttc
    32101 gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta agcccgtcat
    32161 ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc
    32221 gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc gtagcagcga
    32281 atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg ttggcgtcgg
    32341 ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga agcgcggaat
    32401 gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac gcaccgcgct
    32461 taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca tcgcagtgat
    32521 aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat gtctgtcgac
    32581 accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg tcctgtgctg
    32641 ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca ggccattgat
    32701 tatcaactgc ccgtgttgtc atcgacgggc gaactccata agatgtgcag cattcacgtt
    32761 cggacggagt tcctgaccta tgtcccctac tatttatgat attgccaggg ttgcaggcgt
    32821 atcaaaatcc accgtatcac gcgtgctgaa taagcaaact aatatctccc cggaagcgcg
    32881 cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag ccaaacaagc tggcccgcgc
    32941 gctgacctct tcgggttttg atgccattat ggtgatttct acccgttcga ccaaaactac
    33001 ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc accgccaaag ctgaagagga
    33061 aggtttcgac gtgatattgc agacgtcgca caatccggca gaagacttac aaaaatgtga
    33121 aagcaaaatt aagcagaaaa tgattaaagg cattattatg ctaagttcgc cagcggatga
    33181 gtcatttttt gcccaacttg ataaatacga tattcctgtc gtggtgattg gcaaagttga
    33241 aggtcaatat gcccatgttt attctgtcga taccgataat tttggcgaca gcattgcgtt
    33301 gaccgatgca ttaattgaaa gtgggcatca aaatattgcc tgcctgcatg ctccgcttga
    33361 tgtccatgtt tcagtggatc gggtaaatgg ttataagcag agcctggctg cgcataatat
    33421 tgcagtgcgt gatgaatgga ttgttgacgg cggttatacc catgaaacag cactgcaagc
    33481 cgcgcgggaa ttattaagcc agtcaccgtt gcctgaggca gtgtttgcta ctgacagcct
    33541 gaaattaatg agtatttatc gtgcggcagc agagaaaaat attgctattc cgcagcagtt
    33601 agcggtggtg ggttatagta atgaaacgct gtcgtttatt ttaacgcctg cgccaggcgg
    33661 tattgatatc ccaacacagg agttaggtca acaaagctgt gagttattat tccgcttaat
    33721 ttctggaaaa ccgtcaccac agaatattac cgttgccacg catatgacgc tgaaataaat
    33781 accagctacc ttgcctgaat gccggatgcg atgctagcgc atcttatccg gcctacattt
    33841 atagttcgga taaagtactc gtgccgcatc cggcaattta gcccattatc agaacgagta
    33901 atttacgccg acacctgcat aatggaattt atcgctgtcg ccttcgtcgt gatcctgcca
    33961 ctcaaacgcg tattccagcg aaacggaaag cccgttctgg aaatcataac cgtaaaataa
    34021 acctacacgg ttaaaatcat ggccttcacg ttcaatatca tcctgccagt cccagttact
    34081 ccagcgatcc agcccaatgc gcgtatacgg cgtcaccgag tggttgccga gcgtcagcgg
    34141 caaataggcg cgaatttctt gcgtagaaaa ctcaccgtta ttgcggctgt cgtccatatt
    34201 aaagccgcgc tcgagataat agttcactcg caaggcaacc gtttcgttga aggtatattg
    34261 caggcctgtt tccgtttcga ctcgggtatc agcgtaaccg gtcgtgttca gatcgttggc
    34321 aaatttatac atcgacaacc aaccgttgaa acgtaaatcg tcagtcagtt tcacatccca
    34381 gtctggcgcg attttccagc gctgcatatt cgcagtgtct ttacccggtt catcaacgta
    34441 gtgataacca taattacgga aaccgccggt caggccgaaa ctgaaatcat cgttttcgag
    34501 gaactgataa tgcacctcca gctccgggcg atcaaaccac gttccacgtt tacccgcgct
    34561 ataatctacc ggcccttcct gataataggc cagagcaatt ctccacggcc cgttggcggc
    34621 attaaaatag actgaaggct ccgccagccc atccatatct tcgccataac cctcgacgtt
    34681 ttctatttcg tacatcgcgc cgatattaaa gtgccagtcg ttcctttcct cggcctgtgc
    34741 gcaggccatt cccgcacaca tcaccagtgc ggtacagggt aataactttt tcattattgt
    34801 tccttgtatt attctggggg aatgccttcc ccctgtattt agcgaattgc tatttccgtt
    34861 tctgcatcaa agaaatgaca tttcgtcata tcaaaatgaa tagtgatatt ttctcctgca
    34921 tgataatcat ttaacgcacc agcacggacc actaactcgt gccccccaac cgtggtgtag
    34981 agcataaatt ccgcaccggt taattctgcc acgctaattt tggcggaaat gttattttcc
    35041 tcttgcgcgt ccggatgaat atcttccgga cgtattccca tcacgatggg cttatgcaaa
    35101 cttccctgtg ttttcagaac cgctaatttc tcttcgggaa tggttaattt aagcgtttcc
    35161 gtaacgaatt tatcgccatc gatcgtgcca cgaataaaat tcatcgccgg tgatccaata
    35221 aatccggcaa caaacatatt cgctggttgg ttataaaccg ttttcggcgc accaacttgc
    35281 tgaacaatcc catccttcat aatcacaatc cgcgtcgcca tggtcatcgc ttcggtctga
    35341 tcgtgggtca cgtagatcat ggtggtattc agcttctggt gtagcttgct gatctctgcg
    35401 cgcatttgca cgcgcagctt ggcatcaagg ttagagagcg gttcatccat taaaaacacg
    35461 cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct ggcgttgccc gccggaaagc
    35521 gcgcccggct tgcgtttcag gtactcacgc aggccgagaa tttgcgccgc ccagttcacc
    35581 cgctcatcaa tcacctcttt ggcgattttt tgcatcttca ggccaaacgc catgttgtcg
    35641 taaaccgtca tatgcggata caacgcgtag ttctggaaca ccatcgctat attgcgtgct
    35701 ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca gatcgccgcc gctgatctcc
    35761 tcaagcccgg caatcatgcg cagggtggtc gacttaccgc agcccgacgg gccgacaaac
    35821 acgatgaact ctttatcggc aatttccagg ttgaagtcct tcaccacatg cacctggtta
    35881 tcgtagattt tttgaatatg ttgtaacgaa agctgagcca tgttgattcc tttgctatac
    35941 gttttgccag aaggccgata accgcgacca ggtcagagag tccgttgaag gcaacagtaa
    36001 ttgcgccccg gttaagcccg cgccgatccc caccgagcgc ataccgctgg cgttaatggc
    36061 gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc tgcggcggca cgcccagccc
    36121 ggcacaggcg gcgagaaaga tttccgggtc cggtttcgag tttttaagtt gggaagcatc
    36181 cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct aaaatcgtcg gcgcattcag
    36241 ggagacagaa gccagcccaa ccgggatctg ctgtgcacgg agatctgcca gcaaagagcg
    36301 aatgccgggt agaacagcgc tgaccgtcaa ctcgcgcagt gagtggacat agagcagatt
    36361 tttgcgatac gccagttgcg ccctctcctg cgagttaaag tcgccctctt tgcccccgtg
    36421 ttgcagaatg cgccgcagag actcatcgcg gctgatccct tttagggatt cgttaaactg
    36481 cgcatcaatg ctgatgccaa tttcagcggc aatctgctgc cacgcctgga aatgcagatg
    36541 cgcggtatcg gtgattacac catccagatc gaaaattacc ccttgcagtt tcattcatcc
    36601 tcctgatgtg tggtagcggt cccattgaag ggcaaaataa aatcacccaa acagaaaaca
    36661 gattcttcag ccacggatat aagctgaccg ttcaaacgca gtgaaacggg cgcagaagtt
    36721 cgaatcgcaa tacgctgcgc gtcaagagtg acctgtaatt cgcagccctg ccagaacaga
    36781 gggaaagaca actgttgcca ctgctcaggt aacgccggat tgagatgcaa ttcaccgtca
    36841 cgcacgctca ccccggcaaa accctgaatc gcccccagcc agatagcgcc agttgcggca
    36901 gcatggatac catcatcaca actatgcgga tcagcaccaa gatcgatttc agtcccctcg
    36961 cgccagaact gataactttg ggtcagcagg ccacagcgtg cggcaacaat gccgtggatt
    37021 gctttactta atgacgagtc gtgaatagtg cgcggttcat aaaattgcag attggcaaga
    37081 cacgatgccg ctgagaactg ctccggcagc atgtaattga gcatcaccac atcagcttgt
    37141 ttgaggattt gcatctcgtt cacttctgcg cgtgaataat ccagcagaat ggtttgcttc
    37201 cccgccgccg ctttgtattt cgccagatta atcgccggct tagccataaa cgaatcatcc
    37261 tgcggcaaaa cgccgtcggg ctgaatttct ggcagccgca gttctttaag gaacatttcg
    37321 gcgcgatgga taaacgcatc gtcgctacag ccaaactggc gggcaatact cagcgcctgc
    37381 tggacgttgt aatacgccat atagctggtg aaggcgttat tattgacatg ttcggtatat
    37441 tcgtcaggcc caataacatc atgaatttcc agacggtcgt taaccctcac cgcgcggcta
    37501 atccagaact ttgcagtttc cagaagtagc gccatgcctt catgcgcaat gaaactttca
    37561 tcccccgtgg tctgccagta ttgaataacc gcccaggcga tatcggccac cagatgatgt
    37621 tccgcctgcg ccgaggccac tttttgccgc agcccggtgc gaatgttaat ggcggcaaat
    37681 tccggcgtct cttcttcgcc gctgcgcgcg ctttcccacg gaaatagcgc gccctgccag
    37741 ccgttgcgtc gcgctttctc ctgcgcgcct ggcaagttgt gccagcgata acgcagtaaa
    37801 cttcgggcaa ccgtcggatc gctaaacaga tgaaacggta acaaaaatac ttctgtatcc
    37861 cagaaaacgt ggcctttgta gccttcgccg gttaagcctt ttgccgcaat gctgctgcgc
    37921 tcgtcgtggg caggcgtcat gatgcgcaga tgataaagcg cataatctaa cgcttgctga
    37981 tcgtgcgctt cgccgccatt taccgtgata cgacgtttct gccaccattg acgccagttt
    38041 tctgttgata ctgcaagaag ttggtcgtaa ctctgctgcg cgcacatttc aagctggcga
    38101 agcgacgcgc tgccccactc gtctaaaacg gcttgcctgt catcccgcca gtcgatccac
    38161 accagttttt gcaacgtcac tgtctcgcct gcatgaagct gcgcactggt atgttgctgc
    38221 aaacggcgct ctttggcggt atagcattgc tgcacatcac cgctcaccat acagcaacag
    38281 ctgatggcca catcactgcg tccatcctgg gtggtgtaga tcccctgcat cagatgctga
    38341 ccaaacaccc gcacctgggt ttcgtcgaga tgttggcgac cgtggttggt ttgcgtggcg
    38401 tcgatacctg ttgaaatcag cactgacgcg tcggcgtcca gtggcgtaat agtgatttcc
    38461 agcacaatga gcggcagttg gtctgccgaa acaaagcgac ggctggcgat ggtgtaacct
    38521 gtgccgttgc tggtacgcca gacaacgcta cggcgtaatt ctccactggc aaagtcaagt
    38581 tcacgctgcc aggcttcgcg ggataacgag aaaacctcac catttatggc aatctccatc
    38641 cccaagatat caggcaggtt caccagttcg ttgatttcac cttttcccgc ccgatgatac
    38701 agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat cttcttcatg gctggcgcga
    38761 agcccaagat agccgttccc ctgcgccatc agcgatgcat acttgttcag ggtatgctgg
    38821 ctgaaatggg gttctgataa cgttactggc ctggtcataa ttccacacgc gtccctgttt
    38881 ctgcggattg atacaacgcc gccaccagtt gctggatgat gtacccctgc tcggcgtcgg
    38941 ctatcatcac gggcttgccc tgtacgtgat tgataaaggc ttccatgctg cgcaaatggc
    39001 ggttgtcgtc tgccagttcc cgttgcatca gcgtcattaa ttcaccgttg ttatcggtgt
    39061 agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc acaaaagctg acgttcataa
    39121 tcgactgttc gcggatgttg agtgcaaatg acgtttccag ccacagaatg ccgccgttat
    39181 gaaattcaat ggtgccaaat aacgaatctt cgacgctgta agttgccgga tcccactcgc
    39241 caaattgacc gcagctcttt tgcgtgccga tcttttgaaa gctatgcgca ttcacgcttt
    39301 tcaccgccgg aaagcccagc acatacatcg ccgcatccag catatgaatg ccgatgtcga
    39361 taagcgggcc accaccctgc agttctttat tggtaaagac accccagccg ggaacgccgc
    39421 agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc caaaacgccg ttggtcacct
    39481 gttcacgcag ctgttgcgta tcgagcgcaa aacggtggtg aaagtcgtag gccagcacct
    39541 tgcccagttt gcgcgcggta tcgcacattt cccgcgcctg ttctggcgtc atggcgggcg
    39601 gtttttcgca catcacatgg cagcccgctt ccagtgccat cagggtatgt tcgtaatgaa
    39661 aacggttagg tgagcagacg ctaaccacat caggtttcac cgccagcagc atggcctgcg
    39721 gatcgtccca cacggatgca ttaccgtatt tatctgccag cgcctgcgcc tgggaaaggc
    39781 ggctgtcaca gacagccacc agttccagat cgttgcgggt gcagtagtac gaagcatgaa
    39841 ccttatccgc cacctggcct gcgcctatta tcgcgacccg cagcggagag cttgtcattg
    39901 cacttttcac aaagacctct tagcaggtac gcaaccaggc caacgaatca cggtacgcct
    39961 gggcaggatc ttccgcccgg atacgccctt cgtacactac atagccctga tagttatccg
    40021 cgcgcagctg ttcaaacagc gcgtggaaat ccagggtgcc gctgcccggc tggtagcgat
    40081 ggttatccgc aatatgcaca tgaccgagca ggtcgcggtt gtcatgcagc gcctgcgcca
    40141 ggttatcttc ttcgatattc atgtgataga aatcgccgat aatctgtaca tgtttaagat
    40201 cgttttcgac gatgtaacgg cgggcatcgg cgagggtgtt gatcatatga tcctgatagc
    40261 ggtttaacgg ttcgagatac accacggttc cggtacgcgc ggcgacctgt tccagtacgt
    40321 gcagggaatc actcaccatt ttgcggtcgc cgtccaggct acgcggcgag gtcatcggcg
    40381 gtaagcggaa ggtaaacatg ccccacgctg ccggaacgac gataccttta ccccccactt
    40441 ctgccagcgc ttcgagaatg cgttcgatct gctttaagcc attaagacga cgctcttcga
    40501 taaagtcgcc aatccaaccg tcatagccac cgcaggcggt ggtcaccggt aaaccggttt
    40561 ctttgattgc tgctttgact tcttcgagat tgttgaccag cagtttgccg tcaatctcaa
    40621 aaccatcgaa gcccatctct ttgatataac gaaatttctc cagaatgttt tccggaaaga
    40681 atgcctgatt ctgtgtgcct attttcattt tttcattcct ttgaattaaa acgtaacgcc
    40741 cattttgatg ctctgttccg gatgctgatc gacatactgc atgtagcttt ccgggctctt
    40801 ggcaaaggtc actaccgggt cgattaaatc ttcgcaattg agataaccgt tcatcagcag
    40861 ttcccagcag gtttcttcaa tacgcttacg gctccagcgc ggataatccg ggttcggttc
    40921 gctgcacgca cgggagaaaa caattttggc gttattgaaa tgcgcttcgc gtccgaggtt
    40981 aaaaccttca gcaaatggtt tggcaaacgc gacataagag atggtgccgc cataagcaag
    41041 gccacgcagc gccgattgca gcgcgtcggc gtaaccgctg gtttcgataa tcacatcggc
    41101 accctgcttg ccggtcagcg ttttgatctc ttttcctacg tcagtgccaa tggggttaag
    41161 gcagaaatca gcaccgtggc gacgggcaat atcacagcga taggcgatag gatcgacgcc
    41221 aatcaccacc gacgcgccag cgcgtttcgc cagttggatg gctatttgac caatcgcgcc
    41281 aagccctacc accaccacaa aatcacccac gcgcacgttg gcatcgcgca cgccgctcat
    41341 ggcaaactgc gccgggtcgt aacaaacggc gtttttccac gagctgcctt ccggcatttt
    41401 gcgcagcttg tagttattca ccgcgttaat gatgaccgtc tcggagagcg ggccgtagcc
    41461 gcatacgctg tcgcccaccg cgtagtcggt aacgtcgctg ccgcactcga taatgtcgcc
    41521 aaccaccatg ttgccaagct ggaatttgcc aaactcaatg ccgcgcggcg catctgccgg
    41581 acgcggagtg aacatctgcc attcgccgtt aaagtcttca tcgataaacg ggctggcggc
    41641 gcggaagtcg accacttccg ttccgtgttt cggtgcgccg aaacgcacgc ggattttcac
    41701 ttcattggct aaaatcgccc gatcttcata ctcaaccagc gcagcaacac gcggtgctgt
    41761 ggctactaac tttttcattt aaaactcctt gtaaagaaac tggccgcttt agcccttcac
    41821 gccaccggcg gtcaaaccac tcttaataaa acgttccgaa agcgcataca taatcaccac
    41881 cggcaatgcg gtcaccagtg aggccgccat catccgtccc cagatgtaat ctggcgtact
    41941 gaacagcgcg ttcaggccca ccggtaaggt gaaattgctg gcgctggaga ggaaaatcga
    42001 cgcaaacaga tagtcgttcc acgccaccat aaagcagtag acgaacacgg atatcagccc
    42061 ggacatcgcc aacggcacgg tgatgcggaa gataatttgc agacggttga ggccatccat
    42121 catcgccgct tcttcgatct catcggggat ggtgtcgaag tagcttttca gcataaacac
    42181 ggcggtgggc agagtttgcg tcaccatggt gatgatcagc gccatctcgg tgtcgtaaat
    42241 gcccagcgcg gtgatgattt tgaacagcgg caccaccagc aaaatgccag agaacatata
    42301 caccgtgtaa aagctggcat tgatggtcat ccgaccttta aagcgcaggc gggaaagcgc
    42361 gtaagcccca agaatgccga ggaataccgc caccacggat gaaaccaccg acaccaccag
    42421 actgttacgg aagtagtcga caaacgggaa aatcatcggg ttaaaaatgt cgacgtaatg
    42481 ctccagcgtc cactgctgcg gcagcagcgt aggatgcagt gagatcgcct ctttcgcgcc
    42541 cttgaacgag gtcatcagca tcacaaaaaa tggaaacagc gtgatgatga gaaacagcgc
    42601 cagcccgcag taaaaaccga tgcgactgag tgtgcgttta tttgttgcca ttgaggttca
    42661 cccgtttcct ggtcagcaga atgacagcga aaataatgat gaagagcacc accgagatcg
    42721 ccgccgcttt ccctaagtca ttaaaggcaa aggcggtttt gtagagatat acaccgagaa
    42781 tatcgacctt ggtcgtcagc aaataaacat ccgcgaacat gtagaacatc cagatggtgc
    42841 gcagggtcac caccgtcgcc aggaccggca taatcgcggg cagcgtgacg atacgaaacc
    42901 gttgccaggc attagcgcca tccatctctg cagcttcgta cagcgatttg tcgatggtct
    42961 gcaaaatcgc cagaaacgag ataaaggcat acgggaagta gcgccagatg gcgaacagca
    43021 ccaccagcac aaagctactg cccggattat cgaaccacag cggtgcctgc tcatagagat
    43081 gcagaagatc gacgccgagg tagttcacaa tgccgtagcc gttgttgaac atgtatttcc
    43141 aggcaaacac cagcgaaatg gacggcgtta cgtaggagag gatcaccagc gaacgcgcgg
    43201 ttttgcgcag atggaactcg cggttgaaaa acatcgccac tgccagcccg agcacggtgc
    43261 tgcccgccac caccagcgcg gtataccaga ccgtcatcca cagcgagtgc cagaagccgg
    43321 gatcggagag gatacgcaca tagttactca ccccaacaaa ggttgactcg atgttgggat
    43381 tgagcggcag acgtaaaaaa ctgatttcga tattcgacac cattggccag gccaccagac
    43441 cacccagcag taataagctg ggcgcgagaa gcagcagcgc aaagggcata tcggaacgac
    43501 ctgaaaaaag cctgttcatt gcggattcct gtcctgggtt tagtgctgtt cgatcagttc
    43561 atccagcttt ttctggctcg cttgcagcgt gccagggaga tcggctttac ccacggtgac
    43621 gttatgcacc atggagctca ccactccaga ccccgtcaca tcacccatgc gggtaaagtt
    43681 tttatcccct actgcaccaa agacctgaat atttggcagt tcaccgatta gctgattcgg
    43741 tagttcaccc agcgccttaa taacgtcgtt gtctttccag gtggcggtag tgacaaccgc
    43801 tttattcacc ggcagcgcag cacctggcga catcatcacc cagtcggcaa tgttgtctgc
    43861 ctgctccata aaggtgacaa atttttctgc tgcttccgtc tcttcggtct tttgcccggc
    43921 ggtaatggtc agcgaggtca acatgccgta gaccgcagag tttttctcgg ttggcaccac
    43981 gaaaccgacg tttttcgggt cgccttcttt aatcacagcc agaaggatat aggtggagta
    44041 aatcgccatc ggcgcggtgc cgttcataaa ggcgtctttc acttccatga tgtcgttaga
    44101 acccggcata gtgttggcag caaggtcgcg gtaataggtc agtgcctgca tcatctctgg
    44161 tgtatcaagg gtgattttgc cttcggcgtt aaagacgtta gcctggttgg ataacgcaaa
    44221 ctgggagaag gattgttccg tcaacacgct ttctgctgta ggcagcgcaa tgccgtattt
    44281 tttattcgcc gggtcattca gtttctgtgc aacgtccagc agctgttgcc agtttttcgg
    44341 ctcctcaagc cccgcttttg ccagcacatc tttgcgatac cagataccgc caatccaggc
    44401 gctgacagga acaccggtcc atgcgctacc atcttcggta cgcacaatac gcagtacgcc
    44461 atcgtaaaac gcgccttcac caacgttact gatgactgtg gcaaccgctt tgcgatcgat
    44521 aagctgctct ttgtccatca ctttggcgta gtcatggctg gtttcgatca cttccggcag
    44581 cgaaccgcta cgtgaaagag taatgacttt agtgttatag gcatcttctt ccacgggcac
    44641 ttgcttaacg gtgatgccag ggttttcttt ttcaaaacgg gcaatcaatt tactgataac
    44701 ggcctggcgc tcctgctcca ccgaagaatg cataaattcg atggatacat tcgttttatt
    44761 ttcttcttta cagcctgaaa tcagggcgca ggaaaccagt gctgataaca gcacgatttt
    44821 tgatttaatc atgagatgtt cctttttatt taatccacat aacctgccag gggcgcagcg
    44881 ttatttcatt acccaatata tcaacttcac taatcagatc ccgaccatgc agattagtaa
    44941 tatttacatg ctgaatattt ttactgacat taaacaatcc agtcagacaa ttaccatcag
    45001 cgttacttct ttgaatacgc attacggatg aattaatcgt atcgatggta aaattattat
    45061 ccggataaaa ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata
    45121 ccgcatgcct taatgtagct tcatcgttca gttctcgggt tatctcttta ctgtgatatt
    45181 ttttacggtt aatcgcacgg ttatatccga gtttttcgac acctgcgtaa tcattacgcg
    45241 agccaagaat actttgaata tatatcgctg gcacaccggg gaaacttaac aaaatcgcat
    45301 gggcgagaat aaacctggcg caacgttctt catcgctact ctcacggcgg cttaacgcat
    45361 ccatataggt cacatttatt tcatacggac tgcgtgtacc gtcgggatta tttttccagt
    45421 ttactaatgc accttcctgc tgtaacgcct cgaccagctc taatatttcg ctttcaggca
    45481 acaagccccg tagcgggttt agcccgatgc catcgtgaga ggcgaggaag ttaaaccagg
    45541 tggtgttgct ggaaggtagt gtcaggtttt gcgcccacgc acaaagcgcc tcaacgttct
    45601 gtttttgcac cgcatgcagc accagtggcg gcagcgagaa ctggtacacc atatgtgctt
    45661 cgtcatcgcc tgcgccaaag taagcaatgt tgtctttatg cggaacattg gtctcggtaa
    45721 tgatcactgt acctggcgca acgttatcaa taatcgaccg taacagtttg ataatcagat
    45781 gtgttttttc cagatggatg cagctttttc ccggctcttt ccacataaag ccaacggcat
    45841 ccaggcggac atactcagca cctttcgcaa ggtaacagag taaaacatcc accatcgcca
    45901 gcaacacttc agggctacgg taattcaggt caatttgatc gtcactaaag gtggtccata
    45961 aatggcgcgt tgaatgatcg cgcatctgga atggcgttaa taacggtaac gcacgcgggc
    46021 gagtgacggc gctgagatcg gtttgcgggt caacggcaat aaaaaaatct tcaaaacctg
    46081 gatgctgttg taaatagttt ttaaaccatt cactttttgc cgacatatgg ttgcagacaa
    46141 aatcaaacat taaatgactg cattcaccga gttgctgaat atcctgccac tcccccgctt
    46201 cactggcgac ctgatgataa tcaattaccg aaaagccatc atcagatgac catggataaa
    46261 acggcaacaa atgaacatgt gaaaaaatgc tttgcagcca ttgatggtaa aactgattaa
    46321 atgtgggtaa tggttttaaa tcattgctgt gaaattgatc ggcataggta attaacacga
    46381 catcactttc atcccagtgt tttttacgtc gctgtgtaat taatcgtttc gcactctcaa
    46441 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
    46501 ggtaatccgt aattttctgt ttcatgttgc ctttccgtgg tagcggtccc atgaaacgag
    46561 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
    46621 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
    46681 aaataaggtg tgacagaaaa aaaaacggcg cataagcgcc gctcatggtg aattctttta
    46741 acctttgacc ttcggcattg cgatgtcttt caggccaccg gcgttctcaa cgtgggtata
    46801 tcccatctcg ctaaggatct cttttgcttg ccctgactgg cgtccggcat tgcaatacac
    46861 tttcacggtg tcgtttttat ccggaacggc ggtggcaatg cgctctttca cttccttcag
    46921 gggaatattg atggccccct gaacgtgctc ttgctgatac tgctctggaa cacgaacatc
    46981 gatccagtgt tcagcggcga aaacgggcag tgaaaacacc agtgccagag caagtaagcc
    47041 ttttttaaac atgtaagcgt ccttctatgg atgaaaattg ccatccatta tacgcctgag
    47101 atttaacaat ttttgcgaaa ctcccctcac cttttataac gctgtgccag tttattagca
    47161 gcccgactta acagcggttc cagcgccaca gccagcaaca ttttcagcga tcggcgagca
    47221 actgatttta tcgcccagcc cgccacaccc gccgggccat agcgcagtgc ggtaagaagt
    47281 accagcttgc ctgctaattt gaaaccaggc tttacctttt gcccggcctg ttgccagcga
    47341 gtattcataa ctttcctcac agttgacgga aacggctacg taacgtgaaa gtatcggaag
    47401 tgacataacg ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt
    47461 catccagcaa ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg
    47521 gatcaagcgc aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga
    47581 aaatcgacag caccaccagg atacgcacca gttttgccgg tacatcaaaa tagttggcaa
    47641 tcccggcgca gacgccgcgg accatgccct gctgtggaat acgccataat tttttattga
    47701 gattaatgcc cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc
    47761 cagcgcctga atacgttcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg
    47821 ctgctcactt tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca
    47881 gatcggtaaa acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat
    47941 aagtactcct tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga
    48001 ttgtcttgct tcattttggc ttttaattgt gccagttgtt cgctgattgc atcatcggct
    48061 ttcagttcgg caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct
    48121 tccgcttcca tctggtcaat acgacgttcg aaagattcga aacgagccat tgcttcatcc
    48181 agtttgccac tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatgacgt
    48241 aacatcaatg cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag ctcaccgatc
    48301 tctttcttca tgcgtgccag cgtgtcgtcc accagcgtca cttcatgttc caggctctta
    48361 atcagatcgg tcagtttctg tttttcaatt aacgctgcac gtgccagatc ctctctctct
    48421 ttcagcagcg ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca
    48481 atacggcgag tcagctgttt cttttctgcc aacgcgcgcg ccgaagtaga acgtacttca
    48541 accagtgtat cttccatctc ctggatcatc agacgaacca gtttctgtgg atcttccgct
    48601 ttctctaaca gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc
    48661 ataatgttgt cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt
    48721 tgcgtgccaa ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac
    48781 atgccaggat gagttagcga attacactaa caagtggcga atttcatcat ggcagaatac
    48841 aaagataatt tacttggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat
    48901 ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg taaagagctg
    48961 attgccagcc gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac
    49021 tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg
    49081 tttaccggtg cgcaaaaacg tcatcctggg agatttgaac gtgccgacgg cggtacgcta
    49141 tttcttgatg aactcgctac ggcaccgatg atggtgcagg agaaattatt gcgcgtgatt
    49201 gagtacggtg aactggagcg cgttggcggc agccagccat tgcaggtgaa tgtgcggttg
    49261 gtatgcgcga cgaatgccga tctcccggcg atggtcaatg aaggcacttt tcgcgctgac
    49321 ctgctcgacc gactggcttt tgatgttgta caactgccac cactgcgcga gcgcgaaagc
    49381 gacataatgt tgatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct
    49441 ctgttcccgg ggtttacgga gcgcgccaga gaaacattgc tgaattatcg ttggccggga
    49501 aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat
    49561 tatccgcttg atgacatcat tattgatccc tttaaacggc gtccgcctga agacgctatc
    49621 gccgtttcag aaaccacctc gcttccaaca ctgccgctgg atttacgtga gtttcagatg
    49681 cagcaggaaa aagagttgct gcaactcagt ttgcaacaag ggaaatataa ccagaagcgc
    49741 gcggctgaat tactggggtt aacttatcat cagttccgcg cgttgttgaa aaagcaccag
    49801 atttagcgaa gttgagcaat acaacttgca ggatgcggcg tgaatgcttt attcggcata
    49861 cataatcaat acgtcgtttg caggcctgat aagcgcagcg catcagacat tattgcgttg
    49921 ggctattaat cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcacatcc
    49981 gggatggtca gcggatacag gaagcgaatc acgttgccgt atgcgccaca ggtcagcagg
    50041 agcagcccct gcgccagcgc gcgttgctgg attttctgtg caatcgccgc tgacggcttg
    50101 cccgtttgcg gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg taccgcagca
    50161 atggccggaa cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg gcccagttga
    50221 ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga gcaccgcgtg cgcggcagcc
    50281 accgccagcg ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg cgcgtccata
    50341 atattcgcgt taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct tttcgccatc
    50401 gtcattaagt ccggcttatc ggcgtaatga tccatggcaa acagtttacc ggtacgcgca
    50461 aagccgcttt gcacttcatc ggcaatcatc acaataccgt gctcgtcgca caggcggcga
    50521 atggcggcaa ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg caccggttcg
    50581 aaaataatgg ccgcgacctg cttcgcttcg atgtctgatt taaacaagcg ttcgatggcg
    50641 tcgagggagt cctgtgttga aatgccgtgt aaatctgacg gataaggtac gtgatacacc
    50701 gaaccaggga acgggccgaa gccgattttg tacggcgcaa cttttccggt cagcgccatg
    50761 gtcatatatg tacgaccgtg gaagccaccg ctaaacgcaa tcacgccagg tcgtccggta
    50821 tgggcgcgag caattttcac cgcgttttcc accgcttccg caccggtagt gaagaatgcg
    50881 gttttagcct gcccgctcac tggggcaagg gcgttaattt tctccgccag cgtgacgtag
    50941 ctttcgtacg gcacaatctg atacgccgtg tgggtaaact gttgcaattg ctgctccacc
    51001 gccgcgacca gatcagggtg gcgatgcccg gtattcagca ccgcaatgcc tgcggcgaaa
    51061 tcgatgtatt cgttgccctc tacgtccttc agcgtagcgt tttctgccga ctgggcgaag
    51121 aagttacaca tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg atggaattca
    51181 ttgttgctca tgtttcacac cttttgactc ttcactgtta atcggaaatt aaacgcccag
    51241 acgatcgcgc aggctgtaat aagccgcgcc catcgcggta aatggcacgc gcagcgtgcg
    51301 cccgccgggg aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt cggcgtcgcc
    51361 gcgcagcagt tcggcaatca gacgcccggc aagatgggta caggtgacgc cgtggccgct
    51421 gtagccctgc atgtaataga tgttggtatc aaggcgaccg aactgcggca tgcgcgacaa
    51481 ggtcagcagg aagttgcccg tccagcggta atcaattttc acgcccttca gctgcgggaa
    51541 ggttttcagc agtttcggca ccacaaggcg ctcaacgtca tccgggtcgc gcgcgccgta
    51601 gaccacgccg ccgccgtaca gcaggcggtt gtcggcggta agacggtagt aatcaagcag
    51661 atagttacag tcttccacac agtagttttt cgggatcagc gaacgggcta aatcttccga
    51721 cagccgttcg gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg ccagttccgg
    51781 ctctacttta tcgcccagat acgcattccc ggcgacaatc acatacttcg ccgtcacctg
    51841 acctttggca gttcgcacaa cggctggcgt ggtgtgctgg atctgcgtca cggcagaaag
    51901 ttcatacacg cgcccgccgt tgaggcggat ggcgtccgct tcgccaatgg cgaggtttag
    51961 cggatgaata tgcccaccgc tgtgatccag cagcgcaccg gtatagcgat cgctggctac
    52021 ttcacggcga atggcgttgg cgtccagcaa ttccagctgt ttattgccgt agcgttccca
    52081 gttctctttc tgctcttcaa gtgtggcgag ctgtttatca ttcatcgcca caaacaggcc
    52141 gccggggcgg tagtcgcagt caatctgata acgtttgata cgttcgcgga tgatctcacc
    52201 accttcgaac atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc ttttttcgat
    52261 cacgtcgatg tcgcggctgt aggagttcac aagctgcccg ccattgcgcc cgcttgcgcc
    52321 aaagccgatg cgtgaggctt cgagaaccac tacgtcaaag cccgcttccg ccagatgcag
    52381 cgcggaagag agcccggtat agccgccgcc gaccacgcaa acgtcgcagg aaatcgactc
    52441 attcagcgtg tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac tgcttgtatg
    52501 ttcggtcatt tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa
    52561 gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg
    52621 tcatatcgcc gtcgttgtag ttattgacga agacggaacc agccttcagg cggcggctca
    52681 tgcggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg tactggctgt
    52741 cgttggcaag ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc agcaccggac
    52801 cgaaaatctc ttcgcggctt aaggacgcat tcgggtctac atccacaaag atggtcgggc
    52861 cgatggcggc agccagctca gcgttacgac catccagcag cagttgccct ttgctttcgc
    52921 cttcccgaat aaagctatgg accgagtcgg cgtgggcgca gtcgattaag gtgcccatgg
    52981 tggttgcggg atcaagtgga tggcccggct gccagttttg cgcctgctgt tttaacaggg
    53041 ctaagaattc atcggcgatg ctctcttcca gcaacaaacg cgttccggcg atgcacacct
    53101 ggccctggtt gtagaagatg cctgcggcgg tggcgcttgc cgctttttgc agatccggac
    53161 aatcggcgaa aacgaggttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt
    53221 tgctgtcgcc cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa
    53281 tggcgtcgat atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca
    53341 ccacgttcaa cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg
    53401 cactgagcgg tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg
    53461 ggccgagttt ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca
    53521 cgccgaccgg ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc
    53581 catacacttt gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat
    53641 catcacgcag actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct
    53701 cttcggcgtg ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg
    53761 gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt
    53821 cgatatcgac gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg accggatcaa
    53881 cggtttcaaa ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt
    53941 tttcaatggc gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta
    54001 tgactcctgt ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc
    54061 cccgggccgt atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag
    54121 ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg
    54181 attcgctcac ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc
    54241 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    54301 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    54361 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    54421 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    54481 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc attatgaacc
    54541 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    54601 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    54661 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    54721 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    54781 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    54841 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    54901 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    54961 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    55021 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgc ccatgcagag
    55081 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    55141 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    55201 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    55261 ccagttcctg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    55321 taattaacgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    55381 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    55441 tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc atgtggtagc gcaattggca
    55501 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgat
    55561 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    55621 tgctcaatct cacaaagtgg actaaattat cgccattact gcggtttaaa tgacctttat
    55681 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    55741 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    55801 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    55861 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    55921 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    55981 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa gggtgttatt
    56041 tcccggcctc ggtgtttgca atggatattc tgggcaacgt agtagaagaa gcgggtctgg
    56101 gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt tctcggttcc ttaacccctt
    56161 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    56221 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctacgccagc
    56281 gcggattgtt cccggttgta gcggtagagc tggagttcta tttactggat cgtcagcgcg
    56341 acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa
    56401 gccaggttta ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg
    56461 aactggcgca gttacagtta attcccgccg acggtgcggt cgccgaagcc tcgccgggtc
    56521 agtttgaaat caacctttac cataccgata acgtgctgga agcctgcgat gatgcgctgg
    56581 cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg
    56641 cgaagccgta tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata
    56701 atcgtggcga gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga
    56761 tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga
    56821 actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata
    56881 acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg tcataatcac cgcgtggaat
    56941 atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta
    57001 ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg
    57061 aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctaggtgag tttatcgaga
    57121 atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa
    57181 atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga
    57241 aaaacgcgta attccccatg cggggttgtt tcatcggcct gagcggagcc aggcctgaca
    57301 tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc
    57361 ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg
    57421 catggcgagg gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg
    57481 cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg
    57541 ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac
    57601 ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac
    57661 gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc
    57721 ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact
    57781 ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaatcc
    57841 gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat
    57901 caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt
    57961 gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt
    58021 cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt
    58081 cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc
    58141 gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt
    58201 ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg
    58261 cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc
    58321 gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc
    58381 actgcctgaa attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac
    58441 cacgtttgtg aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct
    58501 gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa
    58561 atggcggact ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt
    58621 ctttgattta gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt
    58681 cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa
    58741 ggatcacttc cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg
    58801 ggttaaccta gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc
    58861 atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac
    58921 gccggtgagc gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc
    58981 gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat catccgccct
    59041 tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt
    59101 ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca
    59161 caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca
    59221 gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg
    59281 agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg
    59341 tcagtatcgt tgttcggata aagcgttcac gccgcatccg gctaacaatg acacgcactg
    59401 cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg
    59461 tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg
    59521 ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat
    59581 gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg
    59641 tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg
    59701 aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag
    59761 tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc
    59821 agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg
    59881 ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg
    59941 ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg
    60001 tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca
    60061 acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg
    60121 ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg
    60181 caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc
    60241 gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc
    60301 gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa
    60361 tgccgcaggt agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga
    60421 ccggggaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg
    60481 acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca
    60541 ccgccaaacc gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata
    60601 accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac
    60661 cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat
    60721 cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca
    60781 cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata
    60841 tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc
    60901 ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc
    60961 cggacagttt cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg
    61021 cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc
    61081 tggcggcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca
    61141 ttttgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg
    61201 tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg
    61261 tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt
    61321 cctgctgacc ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgttacaagg
    61381 cgcgtcactg tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg
    61441 tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac
    61501 gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat
    61561 gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact
    61621 gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac
    61681 gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac
    61741 cggttttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat
    61801 gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga
    61861 agtgatccgc ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc
    61921 ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc
    61981 gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat
    62041 gatcaccgaa atggttttta gctggccagg tttagggcgc tggttaatta acgccatccg
    62101 ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat
    62161 tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg
    62221 gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct
    62281 ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg
    62341 ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg
    62401 gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga
    62461 ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg
    62521 gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg
    62581 ccggggcgac acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg
    62641 cgatcccttc gctgctgttg gccattatcg ttgtagcgtt tgccggaccg agtttgtccc
    62701 acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca
    62761 tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa
    62821 cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga
    62881 tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg
    62941 gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt
    63001 atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg
    63061 ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt
    63121 actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt
    63181 cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg
    63241 ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt
    63301 tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg
    63361 gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga
    63421 cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg
    63481 ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt
    63541 ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga
    63601 atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga
    63661 cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg
    63721 tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag
    63781 ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag
    63841 taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc
    63901 agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc
    63961 actgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg
    64021 agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt
    64081 cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa
    64141 gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa gcggtaaaac
    64201 ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg
    64261 gtaaatccac gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat
    64321 tgattgacga tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca
    64381 tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg
    64441 attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg
    64501 aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg
    64561 cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca
    64621 ttattgccga tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc
    64681 tgatgctgga attacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg
    64741 gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc
    64801 gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg
    64861 ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg
    64921 aatcctcctt ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag
    64981 gtgatggata aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa
    65041 cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa
    65101 catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag
    65161 ctggatggtg ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt
    65221 gagtcgcaga actggaccgt gctgcgtttc tggcataacg agattgattg taatgaggag
    65281 gcggtgctgg agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc
    65341 gagggggccg accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa
    65401 tattcggcaa ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag
    65461 cctgtttgtt gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag
    65521 agggttaggg taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctggc
    65581 aactatagct actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta
    65641 ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc
    65701 tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat
    65761 cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca
    65821 gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt
    65881 tctgcagtgc gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa
    65941 agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct
    66001 ggatggtgac tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag
    66061 ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc
    66121 cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg
    66181 tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga
    66241 aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat
    66301 caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt
    66361 tactattgaa gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat
    66421 ctccggtgaa gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga
    66481 attgaaataa tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt
    66541 tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca
    66601 ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa
    66661 aagccactgg tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt
    66721 cccggaagcc gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac
    66781 gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc
    66841 tgaccagctt accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc
    66901 cctgacggtt gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact
    66961 tacgccgctc cccgggcctg attcctccag ggaaagcaat cataggttgg cacaaatcac
    67021 gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca
    67081 tctgaatgac gtcggtctta ctgccgggga ctgtgtgatc attaaccaaa tcattggttt
    67141 tattggcttt caggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg
    67201 ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc
    67261 aatacgctgg cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc
    67321 aacagcagaa ctttgccaac tggccgaaac actctctctc caccctattt cactttccct
    67381 tctcgaaagg ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc
    67441 gttgttatgc gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc
    67501 aaatgaatat aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg
    67561 ggctgaccgt cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc
    67621 acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac
    67681 gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat
    67741 cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc
    67801 cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc
    67861 ttaaacagca actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa
    67921 aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga
    67981 tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg
    68041 aatccgcaga gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc
    68101 agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc
    68161 cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg
    68221 aaatgcgccg tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca
    68281 tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg
    68341 aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag
    68401 atctgaccgc gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg
    68461 cccttttcgc taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc
    68521 caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca
    68581 ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct
    68641 gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg
    68701 atggcaccat tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcaaagc
    68761 tggtgtatga ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg
    68821 aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc
    68881 gtcataacca cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa
    68941 aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag
    69001 aagcgatgat tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg
    69061 gcatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc
    69121 tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca
    69181 aactgcgtcg tgaactggac gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct
    69241 tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag
    69301 catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc
    69361 tgaaagcggt tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa
    69421 tggccgagcg tcgccgtctc aaccggatgg cagaacgtga tgttggtgac tggttatacg
    69481 cacgcttcct gaaagacaaa gccgggaccg acacccgttt cgcagcggaa attgtcgata
    69541 tcagccgtgg cggcatgcgt gttcgtttgg ttgataacgg cgctatcgcc tttattccgg
    69601 cacctttctt acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac
    69661 aaattaaagg tgaaacggtt tacaaagtga ctgacgttat tgacgtcact attgccgaag
    69721 tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc
    69781 attcctcatg gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt
    69841 tttaactgac tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata
    69901 taaaaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat
    69961 ttaaccgcgg actccgcagt taaccggagg atatgcatca tgaaaaccgt tagggagtcc
    70021 acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc
    70081 gatgttttgc gcttttctac caccgaaacc acagaacctg atcgtacttt gcagttatct
    70141 gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt
    70201 ctgaccgtcg atgagagcga tctttctgtg catctggtag gacgaaaaat caataaacgg
    70261 gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca
    70321 cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat agtcattctc
    70381 gacagccggg ggaatattca acgcttcaat cggttatgtg aagattacac agggttgaaa
    70441 gaacacgacg tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg
    70501 gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg
    70561 tggataccaa catgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc
    70621 ggcagtggca aaaacgagat ttttttaatc tgttccggca ccgacattac cgaagagcgc
    70681 cgcgctcagg agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat
    70741 cgtaacgcaa tgcaggattt aatcgatcac gctattaatc atgcagataa caataaagtt
    70801 ggggttgtgt atcttgattt ggataatttc aaaaaggtca atgacgccta tgggcatttg
    70861 tttggtgacc agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac
    70921 caggtgttgg cgcgtccagg tggggatgag tttctggtac tggcatccaa cacctcacaa
    70981 agcgcgctgg aagcaatggc atcacgaatt ttgacccgct tacggctccc ctttcgcatt
    71041 ggtttaattg aagtttatac cagctgttca gtaggtattg cactctctcc cgaacatggt
    71101 tcagacagca cggctattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc
    71161 ggacgaggac aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc
    71221 tggctggata ccaacttgcg taaagcactg gaaaacgatc agttggttat tcactatcaa
    71281 ccgaaaatca cctggcgtgg cgaagtgcgc agtctggaag cactagtacg ttggcagtca
    71341 cctgaacgtg ggttgattcc accgttggac ttcatttcct acgccgaaga gtcagggcta
    71401 attgtgcctt taggccgttg ggtgattctc gatgtcgtac gccaggtggc aaagtggcgg
    71461 gataaaggca taaacctgcg agtggcggta aatatttctg cacgtcagct cgccgatcaa
    71521 accattttca ccgccctgaa acaggttctc caggaactca attttgaata ctgccctata
    71581 gatgttgaac tgacagagag ttgtctgatt gagaatgatg aactggcact gtctgttatt
    71641 caacaattta gccaactagg tgcgcaagtg catctggatg attttggtac cggctactct
    71701 tcactttcgc aactggcgcg ctttccgatc gatgccatca aacttgacca ggtttttgtt
    71761 cgagatattc acaaacaacc tgtctcgcag tcactggtcc gggcgatcgt cgctgtggcc
    71821 caggcattga atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga agatgctttt
    71881 ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt ttgccaaacc gatgcccgcc
    71941 gtcgccttcg aacgctggta taaacgctat ctgaagcgcg cataagtaga tgaatatata
    72001 tacttagcgc tatatcccgt cattatatat cgtattcctg gatgataatt agccgttgct
    72061 gttaaacact tctcttttag cggcaatttt tgcattattc attttccact tttgctataa
    72121 ggatgtcctt gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt
    72181 tagatatcct tgtggcaggc gattaccact ctgcgatcca taatctcgag atacttaaag
    72241 cagaactctt gcgccaggtt gctgaatcta cgccagacat cccgaaggca ccgtgggaga
    72301 tttaacaatc gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc agtatttttt
    72361 tatttagtat tataacgtta taagaattac agcgatgaat tcccgacaac aaactattct
    72421 acagatggtc attgaccagg gtcaggttag cgtaaccgat ctggcaaaag ccactggagt
    72481 ttctgaagtt accattcgcc aggatctcaa caccctcgaa aaactgagtt acctccgccg
    72541 tgcacatggc tttgcagttt cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa
    72601 ctatacgctg aagcgtgaac tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac
    72661 catctttatc gaaaatggta gcagcaatgc cctgctcgcc cggactctgg gcgaacagaa
    72721 gaaaaatgtc actatcatca cagtcagcag ctacattgcg catttgctga aagacgcgcc
    72781 ttgtgaagtc attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc
    72841 tttgacacgc cagtgcatcc agcaggtaca tttcagcaaa gcatttattg gtattgatgg
    72901 ctggcaacct gaaactggat tcaccggtcg cgatatgatg cgtaccgatg tggtcaatgc
    72961 cgtgctggaa aaagagtgcg aagcgatagt cctgactgac agctcgaaat ttggtgctgt
    73021 acattcatac tccatcggtc ccgttgagcg attcaatcgc gtgattaccg attcgaaaat
    73081 acgcgccagc gatctgatgc atcttgagca cagtaaactc accgttcacg tcgttgacat
    73141 ttaaccattt cccttctcta cggatgattt gcagtttggc aaatcatccg ctctaagatg
    73201 attcctggtt gataattaag actatttacc tgttattaac actctcaaga tataaaatta
    73261 ttatcagcga tataacagga agtcattatc acctgcgtga tataaccctg cgcgcgagca
    73321 gatttcacgg aataatttca ccaggcttat tcttagctat tatagttata gagagcttac
    73381 ttccgtgaat cataaattca ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg
    73441 cggctgttct ggcaattact ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac
    73501 gggaccgcaa caccgcaatc ggcgcgggtg caggggcatt aggcggtgca gtactgaccg
    73561 atggcagtac gttgggtaca ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg
    73621 tcggtaaata atcgtaaata aatgtcgatt atcgactcgc aattataata cgcttgattc
    73681 tgttcagtac gaaataactg aataatacgc acagtaaaat attcgaaagc cacggatata
    73741 tccgtggctt tttatgttaa ccgcctgcga gttttacctt catccctttc gcttcaagta
    73801 gtgattttaa taaatcacgc ttatcgccct ggatttcaat aactccatct ttaactgctc
    73861 cgccgcagcc gcattttttc ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga
    73921 gatcgacacc ggtaatcagg caaacgccct tacctttacg tccactggtc tgacgctgaa
    73981 tacgcaccac accgtcgcct ttagggcgta caggggccgc tttgggctca tcaatacgtc
    74041 cggtttccgt tgagtagacc agacggctgt tggaatcact catcatgcac tccgctgtaa
    74101 agaggcgttg atcgctttca gcgtctgcgc tggatctacc gattgcgtta ccgggcgacc
    74161 aatcaccata taatcaacac cagccgacaa cgcctgttct ggcgtcataa tgcggcgctg
    74221 gtcaccagct tcactcccct gcggacgaat gcccggcgta accagtttga actcctgacc
    74281 gaatacctgt ttaaagcgca cagcttcctg agcagaacac accacaccat caaggctaca
    74341 tttttgcgtc agtgccgcca gacgttctgc ataatctgca ggtgacagtg tcatgccaag
    74401 atcgaccagg tcgctggctt ccatgctggt caacactgtc acagcaatca aaagcggtgc
    74461 atctttgcca aacggaacca gtgcctcacg cgctgcggtc atcatacgcg ccccaccaga
    74521 ggcatgaaca ttcaccatcc acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc
    74581 agtgttagga atatcgtgga atttcaggtc aagaaagata tcaaaaccac gctgttgaag
    74641 ttcgcgcaca aactgtggcc caaacaatgt aaacatctct ttgccgacct tcagacgaca
    74701 atcgcgtggg tcgatcttgt cgacaaagga cagcgcgtca tcacgattat gataatcaag
    74761 ggcaacaacc acaggagaat tcgtaacagc gcgggaagaa gatgaagcag ttaacgtcat
    74821 gaccagacct tcttgatgat gggcaccgga agtggcgcga aacaggtaaa cggcgagcat
    74881 tctacccgtg gaaataaaaa attaacagtt gcgatttttc ccctggcagg ctttttctgc
    74941 tccgtcgttg ccggagcaca atggaacatc aataactagt atgttgtaac taaagtcgga
    75001 tttttttaaa aattacaggc catcaagacc gcgaatcggt ttaatggttg accaggcccg
    75061 acaagacgga caatgccagt agagggtgta tgcggtaaaa ccacatttct ggcagcgata
    75121 acgaggctta ctacgtacct tttcgccaac catgtcacgc agcaccatca ggctctcttt
    75181 ggcacgccct tcttccgctt catttaagtg gtaatccatt aacttatgga acacacgcat
    75241 ggtcggatga cgctgaagct ggcgcgtaat atagatctgt gcagcctcac taccgtcgcg
    75301 cgcttcgatg atatccgcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc
    75361 gcgttgcagg aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcaa
    75421 catttccagc gtttcgctga ccagttctct gtcctgggat atcacgcgtt gcaaactttc
    75481 gacggctttg gcgtattctc ctttcgccat aaacacgcgt cccatcatga tggatacgcg
    75541 ggcgctgttt ttatctgccg ccgccccttt ttttagcaag gtcatggcac gatcgagatc
    75601 gtcgctggcc atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg
    75661 tttatcttta cccagcttca ccaggcgttc ggcaacatca attgctttct gccactcact
    75721 ggtagcctgg tagatttgta gcaactgttg cagagcgcca atgcggaagt cagtttcatc
    75781 ggtcagctga ttgaacatgt cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg
    75841 ccctagttgt tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag
    75901 ggtctgatgg atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag
    75961 cgtaaggtgg gcttcaacgg tgcctgtatc ctctttaagc atatcgagaa acaggtctac
    76021 cgctttatcc tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa
    76081 gcggttggct tcatcttgct tattttgttg cgcacttctg cggcccatat accagccata
    76141 ggcagcggct acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc
    76201 cgctgacgag tgcggcacta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg
    76261 ctttatttta cgttcagcgc gcgccaggga aacacgaact cgcagccaga acaggccaca
    76321 aatcaaccaa ccgatagcaa accccgcagc aaacaatacc gccagcaatg tggaaatacg
    76381 gtactcccct tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacccaa
    76441 cgtgaccgaa atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt
    76501 acttcccgtt atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta
    76561 aagtaccatt ttcgcgccgt ctgcgaaatg gcatccacgc gcattgttga tgatttaagg
    76621 gctaaattcg gaaaatcaac cagcgttaac tttcttgttc tcgttgcgct atttcgcgat
    76681 tttcttccgc aggtggtgtt aatggcccac aaattcgttg cgcaagccac gttgccaccg
    76741 ccaccagcgc ccacgaaatc aacgtagcta ctaccagatc gcgtggccaa tgcatcccga
    76801 gcagcaggcg gcttcccatg actcccgttg cccagaccag caagatagca atggttaacg
    76861 ttcgccgacg cggccacagc aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg
    76921 tgtgaccgga aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg
    76981 ggatattttt ctcttcagcc aactgttctt tcactaaatt tccgcgttct gctcgcttta
    77041 aagtgtagaa ctcatcaacc ggaatatgat gtgttttttc cagccagata acaaaaggtc
    77101 gtggttcctg gactttgtct ttgatccagg atttaacgcc ttgtcccaca aggattgcgg
    77161 ccgccagaat ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa
    77221 accagccgaa taaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa
    77281 cccaaaaagc cgcttttagt agccaacttt gttctccagg ttgccaacgc cagccagaaa
    77341 tccatacggc tactggcatg acaagcaata gtgcagctcc cactgcggta cgtctggcaa
    77401 tcgaacgcat ggcctctcct ttttgataag tcccacaatc ataactgaaa acgccagttc
    77461 caggaaaaat tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga
    77521 accccttctc gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca
    77581 tgcagcttaa acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg
    77641 tgggatttga agaactggca accggacacg atcatgtcgc gctagtctat ggcgatattt
    77701 ccgggcatac cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt
    77761 tcagcttgcg ctgcgattgt ggcttccagc tcgaagcggc attgacgcaa attgccgagg
    77821 aaggccgtgg tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata
    77881 aaatccgcgc ttacgcactg caggatcaag gttacgatac cgtagaggct aaccaccagt
    77941 taggcttcgc cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg
    78001 gcgtcaatga agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag
    78061 cagggattaa tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac
    78121 attatctcga tactaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca
    78181 ttgtgtaatt agtttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc
    78241 aacatattac gaatgacata atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc
    78301 gcggtgtcga tacgacaacg gcagggtacg acctcctggc taccatccgc gcgcgtaagc
    78361 gtcaccggaa ccgtcgcacc gggttgtaga ttttgcagat cgccaatatc aatcttctct
    78421 tccccggtta gtcctaacgt tttacgcgtt acgccttgcg gaaattccag cggcaggatg
    78481 cccatgccaa ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga
    78541 attccaagca gacgaggacc ttttgccgcc cagtcacggc ttgagcctga tccatactct
    78601 ttcccggcaa tcaccgccag cggcgtttgt tcctgcttat agcgcatcgc agcatcataa
    78661 atagagacta cgtcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc
    78721 atttcattac ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca
    78781 cgccgcgaac cgtaggagtt aaagtctttt cgctcaacac cccgaccttg tagatatcga
    78841 cccgctgggc tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa
    78901 tcccccagca ttgcgaggat ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc
    78961 tgcatttcat caaagaaagg cgataagcga atataggttg agtcctcctg ccaaccgtag
    79021 gtatcggatc gtgtgacgtt aatttccttc cactctgctg tgccttcaaa aacttctgcg
    79081 tactctttgc ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc
    79141 gatggccaga tatctttcag ataaaccggg tcgcctttac gatcatgacc aataggctca
    79201 gcagccaggt tgatattcat atttcccgcc agcgcatagg caaccaccag cggcggcgag
    79261 gccagccagt tagttttaac cagcggatgg atacggcctt caaagttacg gttgccggac
    79321 agcaccgcac cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc
    79381 ggcccagagt taccaataca ggtggtacaa ccgtatccca caaggttaaa ccccagttcg
    79441 tcgagatacg gtgtcagttt cgcttttgcc agataatcag aaacgacttt cgaacccggt
    79501 gccagcgacg ctttgaccca cggttgccgc ttgaggccca gagttacggc ttttttcgcc
    79561 agcaagcctg cggccatcag cacacttggg ttagaggtgt tggtgcacga ggttatcgca
    79621 gcaatgacca cagcgccatc aggtaactga tactgatgtc cgttcataac ataatcgacc
    79681 ggctggcgat ttttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt
    79741 ggtacatcgg gcagtgcaac gcgatcctgt gggcgttttg gccctgccag gctcgcttca
    79801 acgtcattca tatccagttc taacgtactg gtaaaaatcg gttcatcgcc cgggttacgc
    79861 cacatgccct gcgctttggc atatttttcg accaactcaa cctgatcttc gctgcgcccg
    79921 cttaaacgca tgtaatcgag ggttacagca tcgattggga agaagccaca ggtggcacca
    79981 tattctggcg acatattggc aatggtggcg cgatccgcca gcggtagtga atccagacca
    80041 tcaccataaa attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaaca
    80101 gtgagaacca ggtctgtggc ggtaatacct tcacgtaatt taccggtaag tttaaagccc
    80161 actacgtccg ggataagcat ggaaaccggc tggccta
//
LOCUS       DAZGCS010000006        75920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGCS010000006 DAZGCS010000000
VERSION     DAZGCS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.006"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            331..705
                     /gene="tomB"
                     /locus_tag="ACHK01_000421"
     CDS             331..705
                     /gene="tomB"
                     /locus_tag="ACHK01_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO8095425.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            731..949
                     /gene="hha"
                     /locus_tag="ACHK01_000422"
     CDS             731..949
                     /gene="hha"
                     /locus_tag="ACHK01_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO8095426.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1121..1672
                     /gene="maa"
                     /locus_tag="ACHK01_000423"
     CDS             1121..1672
                     /gene="maa"
                     /locus_tag="ACHK01_000423"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO8095427.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1788..2258
                     /gene="ylaC"
                     /locus_tag="ACHK01_000424"
     CDS             1788..2258
                     /gene="ylaC"
                     /locus_tag="ACHK01_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO8095428.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2422..3972
                     /gene="pdeB"
                     /locus_tag="ACHK01_000425"
     CDS             2422..3972
                     /gene="pdeB"
                     /locus_tag="ACHK01_000425"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO8095429.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4014..4367)
                     /gene="ybaA"
                     /locus_tag="ACHK01_000426"
     CDS             complement(4014..4367)
                     /gene="ybaA"
                     /locus_tag="ACHK01_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFO8095430.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4487..4583)
                     /gene="ffs"
                     /locus_tag="ACHK01_000427"
     ncRNA           complement(4487..4583)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK01_000427"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4746..5057
                     /locus_tag="ACHK01_000428"
     CDS             4746..5057
                     /locus_tag="ACHK01_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO8095431.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5088..5660)
                     /gene="ybaY"
                     /locus_tag="ACHK01_000429"
     CDS             complement(5088..5660)
                     /gene="ybaY"
                     /locus_tag="ACHK01_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO8095432.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5878..6738
                     /gene="tesB"
                     /locus_tag="ACHK01_000430"
     CDS             5878..6738
                     /gene="tesB"
                     /locus_tag="ACHK01_000430"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO8095433.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6786..8072)
                     /gene="amtB"
                     /locus_tag="ACHK01_000431"
     CDS             complement(6786..8072)
                     /gene="amtB"
                     /locus_tag="ACHK01_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO8095434.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8102..8440)
                     /gene="glnK"
                     /locus_tag="ACHK01_000432"
     CDS             complement(8102..8440)
                     /gene="glnK"
                     /locus_tag="ACHK01_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO8095435.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8621..10402)
                     /gene="mdlB"
                     /locus_tag="ACHK01_000433"
     CDS             complement(8621..10402)
                     /gene="mdlB"
                     /locus_tag="ACHK01_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8095436.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10395..12167)
                     /gene="mdlA"
                     /locus_tag="ACHK01_000434"
     CDS             complement(10395..12167)
                     /gene="mdlA"
                     /locus_tag="ACHK01_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8095437.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12197..12655)
                     /gene="decR"
                     /locus_tag="ACHK01_000435"
     CDS             complement(12197..12655)
                     /gene="decR"
                     /locus_tag="ACHK01_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO8095438.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12809..13627)
                     /gene="cof"
                     /locus_tag="ACHK01_000436"
     CDS             complement(12809..13627)
                     /gene="cof"
                     /locus_tag="ACHK01_000436"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO8095439.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13727..15427
                     /gene="ybaE"
                     /locus_tag="ACHK01_000437"
     CDS             13727..15427
                     /gene="ybaE"
                     /locus_tag="ACHK01_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO8095440.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15492..16187
                     /gene="queC"
                     /locus_tag="ACHK01_000438"
     CDS             15492..16187
                     /gene="queC"
                     /locus_tag="ACHK01_000438"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO8095441.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(16239..16637)
                     /gene="fadM"
                     /locus_tag="ACHK01_000439"
     CDS             complement(16239..16637)
                     /gene="fadM"
                     /locus_tag="ACHK01_000439"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO8095442.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16731..17102)
                     /gene="ybaV"
                     /locus_tag="ACHK01_000440"
     CDS             complement(16731..17102)
                     /gene="ybaV"
                     /locus_tag="ACHK01_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO8095443.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17253..19124)
                     /gene="ppiD"
                     /locus_tag="ACHK01_000441"
     CDS             complement(17253..19124)
                     /gene="ppiD"
                     /locus_tag="ACHK01_000441"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8095444.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNEGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19316..19606)
                     /gene="hupB"
                     /locus_tag="ACHK01_000442"
     CDS             complement(19316..19606)
                     /gene="hupB"
                     /locus_tag="ACHK01_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO8095445.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19797..22151)
                     /gene="lon"
                     /locus_tag="ACHK01_000443"
     CDS             complement(19797..22151)
                     /gene="lon"
                     /locus_tag="ACHK01_000443"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO8095446.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22339..23613)
                     /gene="clpX"
                     /locus_tag="ACHK01_000444"
     CDS             complement(22339..23613)
                     /gene="clpX"
                     /locus_tag="ACHK01_000444"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO8095447.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23739..24362)
                     /gene="clpP"
                     /locus_tag="ACHK01_000445"
     CDS             complement(23739..24362)
                     /gene="clpP"
                     /locus_tag="ACHK01_000445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO8095448.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24608..25906)
                     /gene="tig"
                     /locus_tag="ACHK01_000446"
     CDS             complement(24608..25906)
                     /gene="tig"
                     /locus_tag="ACHK01_000446"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO8095449.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26250..26567)
                     /gene="bolA"
                     /locus_tag="ACHK01_000447"
     CDS             complement(26250..26567)
                     /gene="bolA"
                     /locus_tag="ACHK01_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO8095450.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26872..27450
                     /gene="yajG"
                     /locus_tag="ACHK01_000448"
     CDS             26872..27450
                     /gene="yajG"
                     /locus_tag="ACHK01_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8095451.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27494..28969
                     /gene="ampG"
                     /locus_tag="ACHK01_000449"
     CDS             27494..28969
                     /gene="ampG"
                     /locus_tag="ACHK01_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO8095452.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            29430..30377
                     /gene="cyoA"
                     /locus_tag="ACHK01_000450"
     CDS             29430..30377
                     /gene="cyoA"
                     /locus_tag="ACHK01_000450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO8095453.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            30399..32390
                     /gene="cyoB"
                     /locus_tag="ACHK01_000451"
     CDS             30399..32390
                     /gene="cyoB"
                     /locus_tag="ACHK01_000451"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO8095454.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            32380..32994
                     /gene="cyoC"
                     /locus_tag="ACHK01_000452"
     CDS             32380..32994
                     /gene="cyoC"
                     /locus_tag="ACHK01_000452"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO8095455.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            32994..33323
                     /gene="cyoD"
                     /locus_tag="ACHK01_000453"
     CDS             32994..33323
                     /gene="cyoD"
                     /locus_tag="ACHK01_000453"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO8095456.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            33335..34225
                     /gene="cyoE"
                     /locus_tag="ACHK01_000454"
     CDS             33335..34225
                     /gene="cyoE"
                     /locus_tag="ACHK01_000454"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO8095457.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            34410..34721
                     /locus_tag="ACHK01_000455"
     CDS             34410..34721
                     /locus_tag="ACHK01_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8095458.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            34705..35247
                     /locus_tag="ACHK01_000456"
     CDS             34705..35247
                     /locus_tag="ACHK01_000456"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8095459.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(35303..36238)
                     /locus_tag="ACHK01_000457"
     CDS             complement(35303..36238)
                     /locus_tag="ACHK01_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8095460.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            36646..38010
                     /gene="yajR"
                     /locus_tag="ACHK01_000458"
     CDS             36646..38010
                     /gene="yajR"
                     /locus_tag="ACHK01_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8095461.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(38138..38629)
                     /gene="yajQ"
                     /locus_tag="ACHK01_000459"
     CDS             complement(38138..38629)
                     /gene="yajQ"
                     /locus_tag="ACHK01_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO8095462.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            38797..39708
                     /gene="panE"
                     /locus_tag="ACHK01_000460"
     CDS             38797..39708
                     /gene="panE"
                     /locus_tag="ACHK01_000460"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO8095463.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            39671..40261
                     /gene="yajL"
                     /locus_tag="ACHK01_000461"
     CDS             39671..40261
                     /gene="yajL"
                     /locus_tag="ACHK01_000461"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO8095464.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVANGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(40315..41763)
                     /gene="thiI"
                     /locus_tag="ACHK01_000462"
     CDS             complement(40315..41763)
                     /gene="thiI"
                     /locus_tag="ACHK01_000462"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO8095465.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            41969..42211
                     /gene="xseB"
                     /locus_tag="ACHK01_000463"
     CDS             41969..42211
                     /gene="xseB"
                     /locus_tag="ACHK01_000463"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO8095466.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            42211..43110
                     /gene="ispA"
                     /locus_tag="ACHK01_000464"
     CDS             42211..43110
                     /gene="ispA"
                     /locus_tag="ACHK01_000464"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO8095467.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            43135..44997
                     /gene="dxs"
                     /locus_tag="ACHK01_000465"
     CDS             43135..44997
                     /gene="dxs"
                     /locus_tag="ACHK01_000465"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO8095468.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            45177..46151
                     /gene="yajO"
                     /locus_tag="ACHK01_000466"
     CDS             45177..46151
                     /gene="yajO"
                     /locus_tag="ACHK01_000466"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO8095469.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(46205..46723)
                     /gene="pgpA"
                     /locus_tag="ACHK01_000467"
     CDS             complement(46205..46723)
                     /gene="pgpA"
                     /locus_tag="ACHK01_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO8095470.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(46701..47678)
                     /gene="thiL"
                     /locus_tag="ACHK01_000468"
     CDS             complement(46701..47678)
                     /gene="thiL"
                     /locus_tag="ACHK01_000468"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO8095471.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(47756..48175)
                     /gene="nusB"
                     /locus_tag="ACHK01_000469"
     CDS             complement(47756..48175)
                     /gene="nusB"
                     /locus_tag="ACHK01_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO8095472.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(48195..48665)
                     /gene="ribE"
                     /locus_tag="ACHK01_000470"
     CDS             complement(48195..48665)
                     /gene="ribE"
                     /locus_tag="ACHK01_000470"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO8095473.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(48754..49857)
                     /gene="ribD"
                     /locus_tag="ACHK01_000471"
     CDS             complement(48754..49857)
                     /gene="ribD"
                     /locus_tag="ACHK01_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO8095474.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSTQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQVL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(49861..50310)
                     /gene="nrdR"
                     /locus_tag="ACHK01_000472"
     CDS             complement(49861..50310)
                     /gene="nrdR"
                     /locus_tag="ACHK01_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO8095475.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            50461..51000
                     /gene="yajI"
                     /locus_tag="ACHK01_000473"
     CDS             50461..51000
                     /gene="yajI"
                     /locus_tag="ACHK01_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8095476.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSALNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLKSQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            51299..52183
                     /gene="tsx"
                     /locus_tag="ACHK01_000474"
     CDS             51299..52183
                     /gene="tsx"
                     /locus_tag="ACHK01_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO8095477.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(52360..52707)
                     /gene="yajD"
                     /locus_tag="ACHK01_000475"
     CDS             complement(52360..52707)
                     /gene="yajD"
                     /locus_tag="ACHK01_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO8095478.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(52836..53807)
                     /gene="secF"
                     /locus_tag="ACHK01_000476"
     CDS             complement(52836..53807)
                     /gene="secF"
                     /locus_tag="ACHK01_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO8095479.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(53818..55665)
                     /gene="secD"
                     /locus_tag="ACHK01_000477"
     CDS             complement(53818..55665)
                     /gene="secD"
                     /locus_tag="ACHK01_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO8095480.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(55693..56025)
                     /gene="yajC"
                     /locus_tag="ACHK01_000478"
     CDS             complement(55693..56025)
                     /gene="yajC"
                     /locus_tag="ACHK01_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO8095481.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(56048..57175)
                     /gene="tgt"
                     /locus_tag="ACHK01_000479"
     CDS             complement(56048..57175)
                     /gene="tgt"
                     /locus_tag="ACHK01_000479"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO8095482.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(57231..58301)
                     /gene="queA"
                     /locus_tag="ACHK01_000480"
     CDS             complement(57231..58301)
                     /gene="queA"
                     /locus_tag="ACHK01_000480"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO8095483.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            58394..58975
                     /gene="acpH"
                     /locus_tag="ACHK01_000481"
     CDS             58394..58975
                     /gene="acpH"
                     /locus_tag="ACHK01_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO8095484.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(58980..60797)
                     /gene="malZ"
                     /locus_tag="ACHK01_000482"
     CDS             complement(58980..60797)
                     /gene="malZ"
                     /locus_tag="ACHK01_000482"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO8095485.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(60953..62326)
                     /gene="proY"
                     /locus_tag="ACHK01_000483"
     CDS             complement(60953..62326)
                     /gene="proY"
                     /locus_tag="ACHK01_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO8095486.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(62402..63721)
                     /gene="brnQ"
                     /locus_tag="ACHK01_000484"
     CDS             complement(62402..63721)
                     /gene="brnQ"
                     /locus_tag="ACHK01_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO8095487.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(64128..65423)
                     /gene="phoR"
                     /locus_tag="ACHK01_000485"
     CDS             complement(64128..65423)
                     /gene="phoR"
                     /locus_tag="ACHK01_000485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO8095488.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(65481..66170)
                     /gene="phoB"
                     /locus_tag="ACHK01_000486"
     CDS             complement(65481..66170)
                     /gene="phoB"
                     /locus_tag="ACHK01_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO8095489.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            66360..67562
                     /gene="sbcD"
                     /locus_tag="ACHK01_000487"
     CDS             66360..67562
                     /gene="sbcD"
                     /locus_tag="ACHK01_000487"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO8095490.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITADEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            67559..70702
                     /gene="sbcC"
                     /locus_tag="ACHK01_000488"
     CDS             67559..70702
                     /gene="sbcC"
                     /locus_tag="ACHK01_000488"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO8095491.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            70828..72012
                     /gene="araJ"
                     /locus_tag="ACHK01_000489"
     CDS             70828..72012
                     /gene="araJ"
                     /locus_tag="ACHK01_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO8095492.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(72156..73064)
                     /gene="mak"
                     /locus_tag="ACHK01_000490"
     CDS             complement(72156..73064)
                     /gene="mak"
                     /locus_tag="ACHK01_000490"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO8095493.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            73189..74100
                     /gene="rdgC"
                     /locus_tag="ACHK01_000491"
     CDS             73189..74100
                     /gene="rdgC"
                     /locus_tag="ACHK01_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO8095494.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(74178..74261)
                     /locus_tag="ACHK01_000492"
     CDS             complement(74178..74261)
                     /locus_tag="ACHK01_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095495.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(74258..74599)
                     /locus_tag="ACHK01_000493"
                     /pseudo
     CDS             complement(74258..74599)
                     /locus_tag="ACHK01_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(74747..75031)
                     /gene="ppnP"
                     /locus_tag="ACHK01_000494"
     CDS             complement(74747..75031)
                     /gene="ppnP"
                     /locus_tag="ACHK01_000494"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO8095496.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(75103..75780)
                     /gene="aroM"
                     /locus_tag="ACHK01_000495"
     CDS             complement(75103..75780)
                     /gene="aroM"
                     /locus_tag="ACHK01_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO8095497.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDIQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
BASE COUNT        18475 a        20238 c        18787 g        18420 t
ORIGIN      
        1 tttgcggtaa ttctttattc attcctcggt tattacgtca tattcagagc aatcctggta
       61 ttagtgtcac caatttcatc tggcgataat cctgaaatgt tatgaacagt tcgagcaaac
      121 tgcttttacc tgctgcgggt tagtgctagt atgaaaaagt gagtcctgtc ccgattcctt
      181 cctaattgta atttttcgta ataatgcgat gaaaacctgc aaagagtggc ttatagttaa
      241 gctaacaaac gagagggcaa gtccaggtca gtaagttttt tccatcccga aaggtgtccg
      301 ttagttcaac cactaagaag gggacgcgtt atggatgaat actcacccaa aagacatgat
      361 atcgcacagc ttaagtttct ctgtgaaacc ctgtatcatg actgtcttgc aaaccttgaa
      421 gaaagcaatc atggctgggt taacgaccca acctcggcga tcaacctcca gttgaatgaa
      481 ctgattgagc atattgcgac cttcgcactt aattacaaaa ttaagtataa tgaagacaat
      541 aagctcattg agcagatcga cgaatatctg gatgacacct ttatgttgtt cagcagttat
      601 ggtattaata tgcaggatct tcagaaatgg cggaagtcag gtaatcgact attccgttgt
      661 tttgtcaatg cgacgaaaga gaatcctgcg agtttatctt gttagaatta ttacaaccat
      721 gggtagaagt atgtccgaaa aacctttaac gaaaaccgat tatttaatgc gtttacgtcg
      781 ttgccagaca attgacacgc tggagcgtgt tatcgagaaa aataaatacg aattatcaga
      841 taatgaactg gcggtatttt actcagccgc agatcaccgc ctcgccgaat tgaccatgaa
      901 taaactatac gacaagatcc cttcctcagt atggaaattt attcgctaat aaataattcg
      961 ctttcggagc tataaccggc tgtttattaa gaattttata ctttttcacc atgaagacat
     1021 accctatgtg atctttatca cacagatgta atgggaacgt tctcttcact gacttttcgt
     1081 cttactgtgt tgccgcattt tcagcaaccg gaggtcagta atgagcacag aaaaagaaaa
     1141 gatgattgct ggtgagttgt atcgctcggc agatgagacg ttatctcgcg atcgcctgcg
     1201 cgctcgtcag cttattcacc gatacaatca ttccctggcg gaagagcaca cattacgcca
     1261 gcaaattctc gctgatctat tcggtcaggt gacagaggct tatattgagc caacgtttcg
     1321 ctgtgactat ggctataaca tttttctcgg taataatttt ttcgccaact tcgattgcgt
     1381 gatgcttgat gtctgcccta ttcgcatcgg tgataactgt atgttggcac caggcgttca
     1441 tatctacacg gcaacacatc ccatcgaccc tgtagcacgt aatagcggtg ctgaactggg
     1501 gaaacccgtc accatcggta ataacgtctg gattggcgga cgcgcggtca ttaaccctgg
     1561 tgtgaccatt ggtgataacg tcgtggtagc ctcaggtgca gttgtcacaa aagatgtccc
     1621 ggacaacgtt gtcgtgggcg gtaatccagc cagaataatt aaaaaattgt aatcggtttt
     1681 tcgcaactgt tatgcaaaat tgtggtaaat ctgttacttc ccctctacta ttcccacgtt
     1741 aaaatagggt gttccctgga aagttgcaga taccacgaag gcaaacgatg accgaaatac
     1801 aacgcctgct gaccgaaacg attgagtctc tgaatacccg cgaaaaacgc gacaacaaac
     1861 cccgctttag tatcagtttt atccgtaaac atccggggct gtttatcggt atgtacgttg
     1921 ctttttttgc caccctggcg gtgatgttgc agtccgaaac gctgtcaggc tctgtctggc
     1981 tactggttgt attatttatc ctgcttaatg gtttcttctt tttcgatgtc tacccacgct
     2041 accgctatga agatatcgac gtgctggatt tccgcgtttg ctataacggc gaatggtaca
     2101 acacgcgctt tgtacctgcc gcgctggttg aagccatctt gaactctccg cgtgtcgcgg
     2161 atgttcataa ggaacaactg caaaaaatga tcgtccgtaa aggtgaactg tctttttacg
     2221 atatttttac cctcgctcgc gccgaatcaa catcttaagt tagggttaca taccaggcgt
     2281 aaagctctgc gcctggtcaa atgacaatga tcgtttccac ccatcacttc atgaaatacc
     2341 agctctacct ccttatctcc tgccagcctt tttccacaat cagatatact ttccctacac
     2401 tgtgttaata aggatatgct ggtgagaaca cgacatctgg tcggccttat ttcgggagta
     2461 ctgattcttt cagtattgct gcctgtcggc ttaagcatct ggctggccca tcagcaggta
     2521 gaaacatcgt ttattgaaga gctggatacc tattcctccc gcgtcgctat tcgggccaat
     2581 aaggtggcga cacaagggaa agatgcgctg caagagctgg aaagatggca aggcgctgcc
     2641 tgtagcgaag cccatctcat ggaaatgcgt cgggtatctt acagttatcg ctatattcag
     2701 gaagtggttt atatcgataa caacgttccc cagtgttcgt ctctggagca tgaaagtccg
     2761 cccgatacct tccccgagcc aggtaaaatt tcgaaagatg gttatcgtgt ctggttaaca
     2821 tcgcataacg atttaggcat tatccgttac atggtcgcca tgggaacggc acattatgtc
     2881 gtcatgatcg accccgcttc ctttattgat gtcattccct atagctcatg gcaaattgat
     2941 gccgccatta ttggcaatgc ccataacgtt gtcataacca gcagcgatga aattgctcag
     3001 ggaattatta ccaggctaca aaaaacaccc ggtgagcata tcgaaaataa tggaatcatt
     3061 tacgatatcc tgcccttccc ggagatgaat atttcgatca tcacctgggc ttcaacgaaa
     3121 atgttgcaga aaggctggca tcggcaagtc tttatttggt taccgctcgg gttggtgatt
     3181 ggcctgctgg cagcgatgtt tgtgctgcgt attttgcgcc gtatccagtc accgcatcat
     3241 cggctgcagg atgctatcga aaatcgtgat atttgcgtgc actatcagcc gattgtctcc
     3301 ttagccaatg gcaaaattgt cggtgctgag gcactggcgc gctggccgca gacagacggt
     3361 agttggttgt caccagatag ttttattccg ctggcacagc aaacgggcct ttctgagcca
     3421 ttgacgctac tgattataag aagcgtcttt gaagatatgg gcgactggct gcgtcagcat
     3481 ccacagcagc atatttcgat caatcttgaa tccaccgtgc tcacctcgga aaaaatcccg
     3541 caattgctgc gtgaaatgat caatcactat caggttaatc ccagacagat cgcgcttgaa
     3601 ctcactgaac gcgagtttgc cgatccgaaa accagcgccc cgataatttc tcgctaccgg
     3661 gaggcgggcc atgaaattta tcttgatgat tttggtacgg gatattcaag tttaagttat
     3721 ttacaggatc tggatgtcga cattctgaag atcgataaat ctttcgttga tgcgctggaa
     3781 tataaaaatg tcacgccgca tatcatcgaa atggcaaaaa cactgaaact gaaaatggta
     3841 gcggagggaa tcgaaaccag taaacaagaa gagtggctac gccagcatgg cgtgcactac
     3901 ggtcagggct ggctctacag caaggcatta ccgaaagaag atttcttacg ctgggccgag
     3961 caacatttgt gaatttttgg gctgacggca cgccgcacag cccgtcacgc tacctattcg
     4021 tcgatgattg actcgaatcc gccatagatc attcgcttgc cgtcaaacgg catggactcg
     4081 ccgaactctt tcatccgtgg gtccgacatc atcttttgag tagcagcgtc gcggacctct
     4141 tttgaagggt attcaatcca gctaaagaca acctcttcat tctcttccgc tttcaccgcc
     4201 atacgaaaat cggtcacttt gccatccggt acatcgctgg cccagcattc gacaatacga
     4261 agcgcgccaa actctttaaa caatggcgca gccttagcgg ccatttcccg ataggcatcc
     4321 tttttatcgg caggaacggc aaccacaaaa ccatcaacat acttcatcag acatacctcc
     4381 gaagcgtact gcgcagccag acaattcttg cgacgtcagt tgtggttaaa gtttagccat
     4441 gttggggttt tttgacgaaa cggtgggagg cagaaatggg tgggggccct gccagctaca
     4501 tcccggcaca cgcgtcatct gccttggctg cttccttccg gacctgacct ggtaaacaga
     4561 gtagcgttgc gggagaacca acagagcccc cattgagagc gttgagaacc aacgcgcagg
     4621 cgcattatca ctgctgcccc ggctaattgc aacccatcgc gtgccagatg ctggtttctt
     4681 gcgcaatgcg acttcactcg ggcgtttttc cagattatgc tgaaaaatta cctcaggaag
     4741 aaaagatgga aaaagaagat tcatttcccc aacgcgtctg gcaaatcgtc gccgctattc
     4801 ccgaaggcta tgtcaccact tacggtgatg tggcgaaact ggcgggatcg ccccgcgccg
     4861 cgcgccaggt gggcggtgtg ttaaagcgtc tccctgaagg cagcacctta ccctggcacc
     4921 gggtggttaa tcgccacggc acaatttcgc taaccggacc ggatttacag cgtcagcgac
     4981 aggcattact ggcagaaggt gtgatggtat cgggaagcgg gcaaatcgac ttgcagcgtt
     5041 atcgctggaa ctactaaccc tctccgggcg gagagggtta acgtcgctta atattgcgta
     5101 ggagctggaa ctgccgaaga cggattcacc tgagttggtg aagtcgaagg tacggtagtc
     5161 gttgcaccac cgctggcctg aaccggcacg gcggtttgct gcaccggcac caatgtcagg
     5221 tcggctttag ttccgccctt gttgatcacc ggctgaacgg tatcggtaat aaataccagt
     5281 ttgtcattca cggtaatcgc cgcactcaac agaatacgcg cgttcggctg aacatctgcc
     5341 gggttaaatg gcagaacaaa gctgaatggt gactgtttac cttcagtacg caccgctttc
     5401 tgcgccagca cttttgacgg tgcatcggct aacgacgcgt cagaaagtgt cacggtcagc
     5461 acagcatcag gcggcagtgc gactttctga cggatccaga cggtaccgga gacattcggt
     5521 tgctggatag caggttgttg tgttgctgaa atagacgtat ttgcagccgg ggctggcgtc
     5581 tgaatatccg cgcttttatc tgcgcaagcc gccaacgcaa tcgcaaccgc taaaccactg
     5641 gccatgtgca cgagtttcat tcatttctcc ttattatcaa tgcaccagcg ggctaacttt
     5701 cctcgccgga agagtggtta acaaaatagt aacgtcaaca agtgtggcac acatcacgca
     5761 tttctgcctg taattagccc gtaattcaga ccattgcacc catcggacca gtagcaaatt
     5821 tgcgttatac tcaactcact ttggcttgct gcggcagctt tgttactgga gagttatatg
     5881 agtcaggcgc taaaaaattt actgacattg ttaaatctgg aaaaaattga ggaaggactc
     5941 tttcgcggcc agagtgaaga tttaggttta cgccaggtgt ttggcggcca ggtcgtgggt
     6001 caggccttgt atgctgcaaa agagaccgtc cctgaagagc ggctggtaca ttcgtttcac
     6061 agctactttc ttcgccctgg cgatagtaag aagccgatta tttatgatgt cgaaacgctg
     6121 cgtgacggta acagcttcag cgcccgccgg gttgctgcta ttcaaaacgg caaaccgatt
     6181 ttttatatga cggcctcttt ccaggcacca gaagcgggtt tcgaacatca aaaaacaatg
     6241 ccgtccgcgc cagcgcctga tggcctgcct tcggaaacgc aaatcgccca atcgctggcg
     6301 cacctgctgc cgccagtgct taaagataaa ttcatctgcg atcgtccgct ggaagtccgt
     6361 ccggtggagt ttcataaccc actgaaaggt cacgtcgcag aaccacatcg tcaggtgtgg
     6421 atccgcgcaa atggtagcgt gccggatgac ctgcgcgttc atcagtatct gctcggttac
     6481 gcttctgatc ttaacttcct gccggtagct ctacagccgc acggcatcgg ttttctcgaa
     6541 ccggggattc agattgccac cattgaccat tccatgtggt tccatcgccc gtttaatttg
     6601 aatgaatggc tgctgtatag cgtggagagc acctcggcgt ccagcgcacg cggctttgtg
     6661 cgcggtgagt tttataccca ggacggcgtg ctggttgcct cgaccgttca ggaaggggtg
     6721 atgcgtaatc acaattaaaa aaatagccgg aggtaaaacc gtccggctat tttttgcagt
     6781 gcttgttacg cgttataggc attctcgccg tgactgttga catccagccc ttctcgctcc
     6841 tgctcttccg gtacacgcag accaaccgtc agatccgcca gtttgtagcc aataaatgcc
     6901 accacaccgg accagacgat cgtaatggcg atgctttcca gctgtaccag caactgatgg
     6961 cccatcgtca caccttcagc gaagcccacg ccacccagcg aactggcggc aaaaatccca
     7021 gtcatgatac agccgacaat gccacaaacg ccgtgcacac cgaagacatc gcagggatca
     7081 tccacccgca gcaagcgttt gagcatagta acgccccaca agcccgccag accagctacc
     7141 acgccgataa tcaacgcgcc gccaacccca atgtagccac acgctggcgt cacgccgacc
     7201 agaccggcaa tcgcgccaga acacgctccc agcagagaag gcttaccacg cagcgcccat
     7261 tcaccgaaga tccagccaag aattgccgcc gccgttgcga ccacagtatt cacaaatgcc
     7321 agtgccgcga tttcattcgc cgtgcccgct gacccggcgt taaagccaaa ccaaccaata
     7381 tagagaatgg cagtaccggt gaagaccatt ggcaggttgt gcggtttaaa cgcctctttg
     7441 ccgaagccca cgcgtttgcc tatcagatac gcgcccacca gaccggcgat tgcggcgtta
     7501 atatgcacca ccgtgccacc cgcgaaatcc agcgcaccgt gagaagccag caaaccaccg
     7561 ccccacacca tatgcgcaat cggaatgtaa gagagcgtca gccataccac cacgaaaatc
     7621 aacacagctg agaagcggat tcgttccgcc agcgccccaa cgatcaagcc gacggtaatg
     7681 caggcaaacg agccctgaaa cgccacgtgg atatactgat aaatgctgcc catcaccgcc
     7741 gtcagttcga tgttttttag catcaaccag ttaatgttgc cgaagaagtt gttgccctca
     7801 ccaaacgcca gcgagtaacc gtaaaccacc cagagaatac agaccagtgc aaacgtcacc
     7861 gtcacctgcg tcagcatcga cagcacgttt ttgccgcgaa tcaacccacc gtaaaacagg
     7921 gcaatccccg gaatagtcat aaacagcacc agcgcagtac aaatcatcat aaacgcattg
     7981 tcggctttat cggccaccgc aggtgcagcc attaccagtc ccggcagcat cgccagtgaa
     8041 gcaagcccag tttttatcgt cgctatcttc atttttcgtt cctgttgctg tgtgccagag
     8101 attacagcgc cgcttcgtcg gcttcgccgg tacgaatacg aatgacgcgt tgcaattcag
     8161 cgacgaagat tttgccgtcg ccaatttttc cggtgtaagc cgccttactg acgatatcga
     8221 tcacttcatc gagttggtca tcagcaatcg ccacatcaat ttttactttt ggcaggaagt
     8281 tgacgctgta ttccgccccc cggtacagct cggcatgccc tttctgacgc ccgaaacctt
     8341 tcacttcggt gacggtcagg ccctgaatac caatggaaga taacgcttca cgaacgtctt
     8401 ccagcttgaa tggtttgatt atcacggtca ccagcttcat agatcccctc cggtcagaat
     8461 tcggtaatgg ttctgctaca cgaagaaggt attgcaagcg gtgtgccaga aatgaaaaac
     8521 gagaaagaga gcaggaagtt aagaagattg aaaatgaaaa cgcactatga cagtgcacag
     8581 tgcgttacat ttttgcacca acgatgaaat attgcgctat tcaggcgctc aatgattctt
     8641 cttcacgcac gctggctgcc agctcttcgc ccgcaagttg cagttgatac atctgccagt
     8701 agcgtccctg ggccgccagc agttgctggt gagtgccctg ctccacggct tgcccacgat
     8761 gaagcaccag aatggtgtcg gcatcaacaa tggtcgataa gcggtgagca atcaccacca
     8821 gcgtggtatg ttcacgcaca gccgccagag catgttgaat cgcctgttca gtaccggagt
     8881 caatgctggc ggttgcctca tcaaggatca ggatttgcgg cgtctccacc aacacgcgtg
     8941 ccagtgccag cagttgcttt tgcccgacgg agagattatt cccctgctcg cccagcggcg
     9001 tataaatacc atcactcatg ctgcgcgcca gctccgccag ttgcacggtt tccagcgcct
     9061 gccagacgcg ttcttcggag atatcacgcc ccagcgtcac gttggcgagg aaggtatccg
     9121 ccagcaccac cggatcttgc tgcaccattg ccacgccctg gcgcagcgcg ctgtgactta
     9181 gcgaacttaa tggacgacca tcaaggcgaa tctcaccttc cgttagcggg taatagccca
     9241 tcaataaact ggcgagggtg cttttgccac tgccggtatg cccgaccagt gccacaaagt
     9301 tacgcgaagg cacagagaga ttaatgttct ttagcaccag attgtcatcg cgataagcaa
     9361 atgacacgtt atcgacttcg atggtgccac tctgtaacgg gcgatcatca ttgccatatt
     9421 gctggcgcgg tccgtccatc agttcaaaca cgcgctcacc agcaacaaca gcctgttgca
     9481 gcatcgcctg ttgcgtggtc agttcgatta atggttcgtt aagtcgccca agatagctga
     9541 taaacgcata cagcacgccc acttcaatgg tgccgctagc ggagaagcca aacagcatca
     9601 acaagccaca aagaatgagc gatgaaaaca gactcagcag cggacgcagc agaaaaccgt
     9661 cgaggcgcag ggtttgcatc ctggccatat agtgtgaacg actggcctcc cccatacgtt
     9721 cgccaaatcg cgcctgctga cggaactgct ggataacgct catgccattg atgatttcgt
     9781 taaagccgtc gttgatatcc gctagatagg cgcgcacacg acggacaatc ggcgtgctgt
     9841 aacgctggta tatcaccatt accaccagca ccaccgggaa aatcattatc gccaccagcg
     9901 ccattcgcca gtcgaggctg aacatcgcca ccagcatcgc gcccaccagt gcggcgctac
     9961 gcaggacagt tgccactacg gtaacgtaga gatcgcggat cacttcagtg tcattagtga
    10021 cgcgggaaat cacctgcccg acgggttggg tatcaaactc gcttaatggc tggcgcaatg
    10081 cggcatccat cacgtcggta cgcaactgtt gcactacgcc tactgccgcc cgattaaaca
    10141 gcagcgactg cgcgtaatgt agcccggcgg caaacagttg tagcccaaca tacgccgcag
    10201 ccagccctgc aaccattttc aacggcaggt tatttttcgc taccatattg tcgataaaat
    10261 agctgataag cagcggccca ctgacttctg ccgctgccgc aacccacatc atcaggaccg
    10321 caatccccag cggtttacgc cacggcgaac cgtacgctaa cagacgcttg agagtcggcc
    10381 acagttggct aaaactacgc atcgacggcc tcctcgcgaa tttccggagc gtcgtcgagc
    10441 gccgcctcca gttgttgata gcgatacata tcgcgatacc agccgctttg ttgcgccagc
    10501 tcatcatgat tgccacgctg ggcgatatgt ccgtgctgca tcacaataat ttcactggct
    10561 tccgtcagtg ccgaaaggcg atgggcactg atgattaccg ttcttccctg tccccactga
    10621 cgcaggttat gcaggatctg gtgctctgtg cgtccgtcca ccgccgaaag cgcatcatca
    10681 aggatgagga tttccgcgtt gactaataac gctcgagcaa tggagatacg ctgtttttgc
    10741 ccaccggaaa gcatcacgcc gcgctcgccc acttctgtat cgtaaccttg cggtagacgc
    10801 aaaatatcgt catgtacgct ggctaaccgc gcgacatgct caatctcttg ctgggtggca
    10861 ttcgggcaac ccaacgcgat gttgttcgct acggtgtcgg aaaaaaggaa cggtgtctgg
    10921 ctaactaccg ccaggcggct acgccagcta tcgagttgta acttcgtcag aggaatatca
    10981 tgaaagcgaa tatccccctc gctgacgtcg aaatgacgct gaatgagcga caacagggta
    11041 cttttgccgg aaccagtcgg cccgcagata cccagcatct gaccgggttt cagggcgaaa
    11101 ttgacgtttt ccagcgcagg atggtcagtc tgcggatacg tgaactggtg aatatttaca
    11161 tcaagttcgc cacgcccttc cggcacgggt tcgctaccat cgttcaccac cggcgcttcc
    11221 gccagcatcg cacgaatacg gctgtacgca gcactaccac gttccacaat gttaaacatc
    11281 catgccagcg ccagcattgg ccaaatcatc agacctaaat acatcataaa actggtgagc
    11341 tggcccagcg ttaaactgcc ctgcgccacc atccaactac cgccgccaat cgccagcaag
    11401 ttcgccatac caatcgcgat atagatggtt gggtcgaaac gagcatcaat acgcgccacc
    11461 cgcatgtttt ttttgccggt atcttcggca tccgcggcaa ataacgccga ctggcgatct
    11521 tccagaccaa aggctttgat catgcggata ctggtgaggc tttcctgggt gcggtcatta
    11581 agactagaaa acgccgcctg tgccagctta aagcgttcat gcaaggcatc gccgttgcgc
    11641 ttgatcatga tcgccatcac tggcatcggc aacagggcaa ataaggtcaa ctgccagcta
    11701 atttgcgtag acatcataat caacacagcg cagcccatca ccagtgaatc caccagcgtc
    11761 agcacccctt ctccggcggc aaacacgacg cgatcgacgt cattggtcgc acgagccatg
    11821 agatcacccg tgcgatgacg caggtaaaac tcaggatgct gccggcttag ctgacggtaa
    11881 taatcttcac gcagttcaac agccagttga taagacgcac caaacagcaa tacccgccag
    11941 acgtaacgca ggagataaac cacaacggca atcagcacca tggtggcgat ccacatcagg
    12001 atctgcccgg tagtaaagtg ttgttctgtc acgccatcga caacaatacc aaccactttt
    12061 ggcggaacca gttgcagcat cgcgataatg acaagcaagg cgacagcccc gagatagcga
    12121 cgccattccc gacggaaata ccagcttaat tgagcaaata atcgcacgcg ttgtgtcctg
    12181 acctgattct ggatatttat tcgatgggta aagaagtggt gtatttaatc tgttccatcg
    12241 cgaagctgga agtgacgtcc gacagccccg gcacgctgtt taccagacgc ttataaaact
    12301 cgtcgtagcg tttcatgtcg gcaacctgga cgcgcatcag ataatcgtat tcaccagcca
    12361 tgcgccagaa ccccagcact tctggcattt cggtaaccac cgtgacaaag cggcaatacc
    12421 attcgctgct gtgatgttgc gtttttatca gcacaaaggc ggtcaggccg aggcctattt
    12481 tttccggatc cagcagggcg actttgccga taaggatacc gtcgtcctcc agccgtttca
    12541 ggcgcttcca gcaaggggtg gttgtcagat taacggcttc agccagtgcc tgcaaagaga
    12601 gggtgcaatc ctgctgcagt aaggccagca gcttacggtc aattttatct aacatagccc
    12661 ttccacggag aatttttttc tcttttaatt ctattttaaa ggtgaaatcg cgacaattta
    12721 ttctgtgcgg tttaaacacc acggcacaaa atgacaaatt caacgcaaaa aaaaaccggg
    12781 caattgcccg gtgctggaag ggatctcgtt attcggggga ataaggtaga tgtggatagt
    12841 ccagccagtg cgtcaaatag tgagagacag cctgatttcg gcaatgtcca atcaccggta
    12901 aatgcgggag ctccgcgcgc agttgcggca tcgcattgcc cataataaac ccgctaccga
    12961 cgctgcctaa catttcgcga tcgttcatcg catcaccaaa ggccatgcaa tcgcgcaacg
    13021 ataaacctaa atgttgggtc agcaccgtca atgcagcgcc tttattgcag cccaccggca
    13081 gcacttcgag gcaatccgtg gcggaaaaac acaaatgtgc acgctcgcct aatgcttcgt
    13141 atagctggat ctgcaagcgt gtaagatcgt cgtgatcgcc acagaagcag atcttggtga
    13201 cgctgccgag tggcattttt ttgacatcga ttatctgata acgaaaaccg ctatagacaa
    13261 atgcctgcaa caacgcaggg atctctttcc cggtaaacca accgtcgtca ttgaagatat
    13321 gcatgctggc tcgggtatcc cattgctgat acagcaccag ctccgcgaca tccgcaggta
    13381 aatcatcacg atgtaaaagt tcaccttcca gagaatgcac gcgcgttccg ttgccggtaa
    13441 tcaaatatgc atccagcgat agcgccccga gaatatgctg catctccagc gcatgacgcc
    13501 ccgtggcaaa agtgagggta atgtcgcgtt cacgcagtcg cgccaaagta gagagggttt
    13561 tctcacctaa atgatggtca ggcatcaata aagtgccatc catatcaaat gctgccagac
    13621 gagccatttc tttctccact ctgtgacacg gttaatggtg attgagtatc acctgatata
    13681 tacggaagta atagtgaata gttaaataat attgttccgg gtttatatgc gattgctcaa
    13741 ccgtcttaac cagtatcaac gtctgtggca accttcagcc ggaaagccgc aaaccgtcac
    13801 cgtcagcgaa ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg
    13861 tcaggcacag gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg
    13921 acaattacgc tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact
    13981 ggaaaccgga aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg
    14041 cactctgtta cagccgttta tgggcggaca atggcaaaac gatacaccca cgttgcgtat
    14101 tccctactat cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca
    14161 gcatctcgcc gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc
    14221 gattggcgat ttagcgcatc actgggaaac ctctactgac gggttacgct gggactttta
    14281 tcttcgttca accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca
    14341 gcgattattg atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg
    14401 tattgaagtc acccatccgc agtgtctgac cttcttttta catcgccccg attactggct
    14461 tgcgcaccgg ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg
    14521 cactggtcct tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga
    14581 ttattaccat ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct
    14641 tttcgaaaaa gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc
    14701 ggaggagctg caacgggtca gccaggtcag tagtggcatc agtttaggtt tttgctattt
    14761 aacgttgcgc aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat
    14821 tcatcaatcc ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca
    14881 tgcattactg ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc
    14941 gaaaaccttg acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct
    15001 acaggcgaca ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaaa
    15061 ctgggacgac acgaccctac tggcacacgc agacctcatg atgggcgaca gattaattgg
    15121 cgaagcaccg gaatatactc tggagcaatg gctacgttgc gatccactat ggccacatgt
    15181 tttcgacgct ccagcatatg cacatctgca atcgacgctg gacgcggtgc aagtaatgcc
    15241 tgatgaggaa aaccgattta atgccctgaa agcggttttt agccagttaa tggcagacgc
    15301 gacgctgacg ccgctgttca actatcacta tcgcattagt gcccctcccg gcgtgaacgg
    15361 tgtgcgactg acaccgcgcg gctggtttga atttaccgaa gcctggcttc ccgcgccgtc
    15421 gcaatgaata gctggtccgg gttaatcgca ggcgttacca taacgctttt attgttcaag
    15481 caggattatc tatgaaacgt gctgtcgttg tgttcagtgg aggccaggat tccaccacct
    15541 gtctggtgca ggcattacaa caatatgatg aagtccattg cgtgacgttc gattacggtc
    15601 agcgacatcg cgcagaaatc gacgtggcac gcgaactggc gctgaaactg ggagcacgcg
    15661 cgcataaggt tcttgatgta acgctgctca acgagctggc ggtcagcagc ctgacacgtg
    15721 acagcattcc ggtgcctgat tatgaacctg aagccgatgg catcccgaat acgtttgtcc
    15781 ctgggcgtaa tattttgttc ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag
    15841 aagccgtaat taccggcgtc tgtgaaacgg atttctccgg ctacccggat tgccgcgatg
    15901 agtttgtgaa agcactaaac catgccgtca gtttgggcat ggcgaaagat attcgttttg
    15961 aaacgccgct gatgtggatt gataaagcgg aaacctgggc gctggcagat tattacggca
    16021 aactggattt agtccgtaac gaaacgttga cctgctataa cggcattaaa ggcgacggtt
    16081 gcggtcattg tgcggcatgt aatttacgcg ccaacggttt gaatcattat ctggccgata
    16141 aaccgacggt gatggcagcg atgaagcaga aaaccgggtt gaggtaatta ttccgggtgt
    16201 cgccggatgc ggcttgagca tccggcacca caaaacgttt acttaaccat ctgctccagc
    16261 ttttcgcgca attccccttc cagagctaat gctttctgcg ttttaagatc aatacaaaca
    16321 aacgtaataa gcgcatccgc taccacctgc ccttccggct ccagtgtaat gacctggctt
    16381 aagatgccgc ttttaccgtt taattgctgc aactgactgg taatagttaa caggtcactt
    16441 aataccgctg ggcgacgata gttaatattg atattgacca cgacgaaggc gatgttatgg
    16501 gccgtcatcc actgaaaact gtcgctattt tccaacccat cccagcgggc ttcttcgaga
    16561 aattcaaggt agcgggcgtt gttgacgtgc tggtaaacgt cgagatgata tccacgaact
    16621 ttgatttgtg tttgcatagc gcaataacct tacgttgttg ttataagtac agaggtcata
    16681 actggtatga cctcttcagt ctggcaaaaa attgccacta tgcaaattaa ttacagggtt
    16741 aataccgcca gattacgttc caccagcgaa ttgcccatcc ccggcacctg ctttagatcc
    16801 tccaccgttt taaacggacc gtactcttcg cgataactga caatcgcctg cgctttcttc
    16861 aggccaacgc cattcatcgc gcgggctagc tcttccgcgc tggcattatt aatgctgacc
    16921 cgggtgcctt cttcgtcact ggctttcgcc ggtactgctg ctttactttg tgctgcagga
    16981 gcttccgctt tggtttctac cgttgtcggt ttcgccacag aagctgccgc cagcgcgcta
    17041 tgagacattc cggcacaggc cagggacagg gtaatgagca gtgctttaat tccgtgtttc
    17101 atactgtttt ctccttgttt gttaacagtg gaatcacctt aacggcagtg aaaacagtaa
    17161 acaaatggca atgttcagaa atggaaaagg ccgcgaaagc gaccttttct tgttacagct
    17221 gcaacgcatt tttttgcgag gcggcttcag gattattgct gttccagcgc atcgccaatt
    17281 ttgattttcg cctctttacg caggttactc atcagagctt caaagacgat ttgtgcgttg
    17341 ttctgggtga taccctgcac catcgctttt ttctgatctt ccggcattga accttgtttc
    17401 acttcatcca gcgccagcag aaccacatta ccttgcatat cggtcgccat accgtagctc
    17461 ggtttgtctt tcgctggcag tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg
    17521 gaacggctta agattttcgg ctcgccaaat ttcagaccgg cagcctgcat agcttccgca
    17581 cctttgccgg ctttcaaatc aaccagcagt ttctcagcat ccactttcgc ctgttgttca
    17641 gctttgttgt gctgaaccag tgccttaact tgttcctgaa catctgccaa cggtttcacc
    17701 gcttccggtt tgtgctcgct gatgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg
    17761 atgtcagagt tgatgcccgg cgcgccgttt tcacctacca gaccgccgtt aaagatagcg
    17821 tcggcaaccg gcttgaagtt caactcttcc ggcaggttat ctttgctaaa ccaacccgtc
    17881 tgagtggctt taacgccggc agcttgctct gcaccggcca gagactcggt gtcgttgctt
    17941 gccgcatcgc tcactttctg ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc
    18001 actttcgccg caatgtcgtc acgtacttcg tctaacgatt tcactttcgc tggctgaatg
    18061 tcgtccagac gtacaatcag gaaaccgacc gaagatttga tgacaccaga cagttgccct
    18121 ttttctttca gaccagcatt tttcagttcg tcggggatag tggcatcttc taaccaaccc
    18181 atatcgccgc cgttacgggc agagataata tcggcagatt tttctttggc taatgcagca
    18241 aaatcaccgc cttcattcag ctcatcaagt accgctttcg cttcatcttc agttttggtc
    18301 tggatgatgc tgtagcgggt acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag
    18361 tagctctgga tatccgcatc gctaaccggt tgctgcatcg ttgcggcatc cagcttgatg
    18421 taactcacgc ggaattgttc cggcgtcatg aaattgtttt tgttttgttc gtagtagctg
    18481 gcaatctcct gttcggtcac aggctgcttc gccgccagcg cgttaacatc gatagtcgcc
    18541 tcacgcacca cgcgttgttg cgcgaccagt gccgccagct cgtcggtttc acctttcagc
    18601 ataaaatcgg taccggcaac accgttaatc agctgttggg tagtgagctg gttacgcagc
    18661 gcctgggcgt actgatcggc ggtcatcccc atctggttga ggataccgtt atagcggctg
    18721 ttatcaaatt tgccgtcaac ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc
    18781 tcatcactga tacccagttt cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc
    18841 agacgattca gcacctgttg acgcagggtt ttcatatagc cttcgttcgc tgccagctcg
    18901 gagtattgat cgcccagctg ttgctgcatg cgattacgct cgctgttaaa ggcgttctcg
    18961 aactgcccac ggctgatttc ctggtcattc acttttgcgg cgtagttatt gcctccgcca
    19021 atcaggtaac cactcacgcc ggtcaatatg aacgacacga taatgatacc gaaaataatc
    19081 ttgagcacga gactgtttgc agccgtgcgt aagctgtcca tcatggtgta acaacactcc
    19141 gctgtagatg actgtgtacc tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca
    19201 agaaacgggg gcaattgtca gcccacaaga cgcagaaagt caccgaatac aaataaaaaa
    19261 ggcacatcag tagatgcgcc cttgaacttc gtcacatccc caccggggac aacgcttagt
    19321 ttaccgcgtc tttcagtgct ttacctgcac ggaagctcgg tactttagca gcagcgatgg
    19381 tgatctcttt accggtctgc gggttgcggc cagtacgggc agcacgctct ttaacggcaa
    19441 aagtaccaaa acctaccagt gctacatcat ccccttcttt cagagattca gttacggaag
    19501 caataatagc atctaacgca cggccagccg cagctttaga gatatcagcc cctgcagcaa
    19561 tcttgtcgat caattgagat ttattcactc ttctcttcct ctttataatt tatatcgcac
    19621 ttgaatcctt caaggtacga acgcgcagca gttatatcag gccagccatc cccttacaac
    19681 accccttaat aagggcaagc ccgatccgcc atctaactta gcgagacaaa aaaaggctgg
    19741 caagcccgaa ttagcctgcc agccctgttt ttattagtgc attttgcgcg aggtcactat
    19801 tttgcagtca caacctgcat accagacggt tcattttgta gcgccagagt cagaacttcc
    19861 tcaatgcgct tcacaggatg aatgtccaga tcggcaatta cgttgtcagg aatctcttcc
    19921 agatcgcgtt tattttcgaa cggaattagc actgttttaa tcccgccgcg atgcgctgcc
    19981 aggagttttt ctttcaaacc accgatcggc agtacctgac cacgcagagt gatctcaccg
    20041 gtcattgcca catcggcacg aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac
    20101 atagcaatac cggcactcgg accatctttc ggcgtcgcac cttccggaac gtggacgtgg
    20161 atgtcacgtt tttcgtaaaa atcagggttg atccccagtt tttccgcacg cgcacgaacc
    20221 accgttaacg ccgcctgaat agactcctgc atcacttcgc cgagcgaacc ggtataggtc
    20281 agtttgcctt tacccggaac acatgcggtt tcaatggtca gcaagtcacc gcccacttcc
    20341 gtccacgcca gaccggttac ctgaccgaca cggttttcgt tatcagcgcg accatagtcg
    20401 aaacgctgaa caccgaggta gtcatgcagg ttatcgccgt taatttcgat atgttttaat
    20461 gacttatcga gcagtaactg cttaaccgct ttgcggcaca gtttggagat ttcacgctcc
    20521 agaccacgca cgcccgcctc acgggtgtag taacgaataa tgccgataat ggcgctatcg
    20581 tcgacggtca gctcaccttt tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga
    20641 cgtttggcga tgttcagttt ttcatcttcg gtataaccgg agaggcgaat cacttccata
    20701 cgatccagca gcggtgccgg aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg
    20761 ctgagatcgt aatccacttc caggtagtgg tcgctgaacg ctacgttctg ctctggatcc
    20821 agcacttcaa gcagtgcaga ggccggatcg cctcgcatgt cagaagacat tttgtcgatc
    20881 tcatcgagca ggaacagcgg gtttttcacg cccactttcg ccattttctg gatcagttta
    20941 cccggcatag aaccgatgta agtacggcgg tgaccacgga tttccgcttc atcacgcacg
    21001 ccgcccagcg ccatacggac atatttacgc ccggtggctt tggcaatgga ctgaccaaga
    21061 gaggttttac ctacccccgg cggccctacc aggcagagga tcggtccctt gattttgttg
    21121 acacggcttt gaaccgcaag gtactcaagg atgcgatctt tcacgcgctc cagaccataa
    21181 tggtcggtat caaggatttc ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca
    21241 ttccacggta cctgtaccat ccagtcgata taaccacgca ctacggtcgc ttctgccgac
    21301 atcggagaca tcattttcag cttctgcaac tctgcttccg ctttctcttt tgcctctttc
    21361 ggcatcttcg ccgcgtcgat tttgcgcttc agggcttcgt tttcgtccgg cgcgtcgtcc
    21421 atttcaccga gttctttctg aatagctttc atttgctcgt tcagatagta ctcacgctgg
    21481 gatttctcca tctgcttttt aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg
    21541 atttccgatt ccatcattgc catcagatat tccagacgtt cgttaacgtc ggacatctcc
    21601 agaacggact gtttgtcagc cagtttcagc ggcatatgtg cagcaatggt atccgccaga
    21661 cgcgccggat cgtcgatgct attcagcgac gtcagcactt ctggtgggat ttttttgttc
    21721 agcttgatgt agccttcgaa ctggctgatt gcagtacgca ccagcacttc ctgttcccgc
    21781 tcatcaatgg tcggcgactc cagatactcc gccttcgcag aaaagtgttc gccattgtca
    21841 gagagcgcag aaatacgcgc gcgctgtaac ccctcgacca gcactttgac ggtgccgtca
    21901 ggcagtttca gcatctgcaa tatagaggcc acggtcccga cggtgaaaag atcgtttaca
    21961 cccggctcat ccgttgaagc ttctttctgc gcgaccagca taattttttt atcatggtcc
    22021 atcgccgctt ccagacaacg gatagatttt tcccgcccga caaataaggg gatgaccatg
    22081 tgcggataaa ccaccacatc gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc
    22141 tcaggattca tagagctctc tcttagttta atttccgcca ggtaatcaga tgacacgact
    22201 gtgcttcacg ccatctatta acatgtacgt cagtatatgg ggatgtttcc cccacattca
    22261 acgccgagaa tagaggaaaa attaaagggg agataaaatc cccccttttt ggttaactaa
    22321 ttgtatggga atggttaatt attcaccaga tgcctgttgc gcttccggct tgccataaat
    22381 cagcaacggt ttgctttgac catcaattac cgactcgtcg ataaccactt tttcgacatc
    22441 ttccatggac ggcagatcgt acatggtatc gagcagtgcg gcttctacga tggaacgcag
    22501 gccacgggca ccggttttac gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc
    22561 acggaattcc agatccacgc cttccagatt aaacagcgcc tgatactgct tggtcagggc
    22621 gtttttcggc tctttgagga tctgaatcag agcttcttcg ctcagttcat tcaacgttgc
    22681 gacaaccggc agacgaccaa tgaactcagg gatcagacca aacttgatca gatcttccgg
    22741 ttcaacctgc gccagcagct cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc
    22801 gccaaaacca atgccggagc cggtttctac acgatgggaa atcactttat ccagaccggc
    22861 aaacgcaccg ccacaaataa acaggatctt agaggtatca acctgcaaga attcctgctg
    22921 cggatgttta cgcccacctt gcggtggaac agcagctacc gtaccttcga tcagtttcaa
    22981 cagtgcctgc tgtacgcctt caccggaaac gtcacgggta atagacgggt tgtctgactt
    23041 acgagaaatc ttgtcgattt catcgatgta gacaatcccg cgctgcgctt tctgtacgtc
    23101 gtaatcgcac ttttgcaaca gcttctgaat gatgttttca acgtcttcgc ccacataacc
    23161 ggcttcggtc agcgtggttg cgtcggccat ggtgaacggg acgtccagca ggcgcgccag
    23221 cgtttcagcc agcagcgttt taccggaacc ggtcggaccg atcagcagaa tgttactttt
    23281 gcccaactcg acaccattgc tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac
    23341 cgcgaccgcc agcacttttt tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt
    23401 gcgaatttca tgcggcgtcg gtagcgcact gcgttcacga tgcggtgcaa cttctttaat
    23461 ctcttcgcga atgatgtcgt tacataaatc aacacattcg tcgcagatat acacggatgg
    23521 accggcaatc agcttgcgca cttcatgctg gcttttgccg caaaaagagc aatacagcaa
    23581 tttgcctgag ccatctttgc gtttatctgt catgggtcaa aacctcttct ttgttctttg
    23641 tgccgcacac gacgacgcaa atgccattct caggcgcaag ccgcttacag cgttgtgccg
    23701 ccctggataa gtatagcggc acagttgcgc ctctggcatc aattacgatg ggtcagaatc
    23761 gaatcgacca gaccgtattc caccgcttca ggggcggaaa ggaagcgatc gcgctcggta
    23821 tcacgttcaa tctgttctaa tgattgaccc gtatgaagcg ccataagttc attcatgcgc
    23881 cctttaactt tcagaatttc acgggcatga atttcgatat cggtcgcctg gccctggtag
    23941 ccgcccaacg gttggtgaat catcacgcgc gaattcggca ggcaaaaacg tttacctttt
    24001 gcccctgcgg tcagcaagaa agcgcccatc gaggccgcct ggcccataca gatggtgctg
    24061 acatcaggct tgataaactg catggtgtca tagatagaca tcccggcagt gatcaccccg
    24121 cctggggagt taatgtacag atagatatct ttttctgggt tttccgcttc caggaacagc
    24181 atctgcgcca caatcaggtt agccatgtgg tcttcaacct ggccagtcag aaaaatgacg
    24241 cgttccttaa gtagacgaga atagatatca aaagagcgct caccgcgtga ggtctgttca
    24301 atgaccatcg gcaccagcgc catatggggt gcaaagttat ctcgttcgcc gctgtatgac
    24361 atttccgtct cctggataaa attgaaaaaa cctgctgtac cgattgtaac cttatggacg
    24421 gattatcagc tagtccctgt gttatgggta cggcatcacc atatttcaag cataacaatc
    24481 ttttgttgtt acgctaacac cgaaagaggc ttttcgcacg gttccatgca aaattcatga
    24541 caaaaaaaag cccaccacct ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct
    24601 gcgtaaatta cgcctgctgg ttcatcagct cgttgaaagt ggtttctttt tcagtcactt
    24661 tcgctttcgc cagtacagct tcaacagcct gttcttccag agcaacattg cgcatgttgt
    24721 ccatcagttc tttgtttttg ctgtagaact cgataacttc tttcggatct tcgtacgcag
    24781 aagccatctc ttcgatcagg cctttcacgc gctcttcgtc agctttcagc tcgttggtgc
    24841 ggataacttc gcccagcagc aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca
    24901 gttcgcgcgg cagttccaga gcttgttttt cgttgccacc gaaacgctgt gcagcctggc
    24961 gacgcagaac gtcgatttcg ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt
    25021 ttaccagacc ttcgatcgcc tgagacttaa cgcggttacg gatggcgctc ttcagctcgc
    25081 gctccatgtt tttacgcact tcagcgcgca gaccttctac ggaaccatct tcaacgccga
    25141 aacgtttgat gaattctgca gtcagttccg gcagttcacg ctcttcaact ttcttcaggt
    25201 tgatagcgaa tttcgctgct ttacctttca ggttttctgc gtggtattct tccgggaagg
    25261 tcacgtcgat ggtgaactct tcgccagctt tgtggccttt gataccgtct tcaaagcccg
    25321 ggatcatacg accctggccc atcgccagta cgaaatcaga cgctttaccg ccttcgaact
    25381 cttcgccgtc tacagaaccg gtgaagtcga tggttacgcg gtcttctgct tcaacagcgc
    25441 cgtctttttc tttccaggtc gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt
    25501 cagcgtcggt cacttcaacg atcggttttt caacttcgat cgcttccaga ccctgcagtt
    25561 caacttccgg ataaacttca aactctacag agtaagtgaa gtcttcaccc agcttgtatt
    25621 cgcccggaac ataagtcggt gcgccagccg gattgatttt ttctttaatg atggcgtcaa
    25681 tgaagttacg gctcatcagg tcacccagaa cgtcctggcg tacagacgcg ccataacgct
    25741 gagcaacgat attcattggc actttgcctt tgcggaagcc gtcaatacgt acttttttcg
    25801 caacgttgac cagctcgctt ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg
    25861 ttacacggcg gccaaggcct tgagtggttt caactgaaac ttgcatcttg ttacctcaaa
    25921 aaatcacagt gctcggtcaa ctcttacccc gcaagcacag gttgttggct tcgcggaacc
    25981 gggatgttct tataatcaat cacattccct gttaccagaa tcatcccgaa agcattcaaa
    26041 taggacgcgg cattatagcg gcatcacttt tatgagtcga gaacggttat tgcgcgctgt
    26101 cgcgtatttt ttcacaattc ccgggcaatt ttcgtctgaa tgctgggcaa aaagcgcaaa
    26161 attcagacaa aacaaaacgg cccgtaggcc gtttttcata aaatctttag caacatactg
    26221 gaaaagcgcc gacagttgca aatgcgtttt tacgcgatgc ttcctgctcc acgacaggga
    26281 ggagaggcaa agacggtgtc ctgcaaccct tcccactcct taatagtgta agtatgcaga
    26341 gccagcgcat gaacggtagt agagagttcc tccgctaaag tactgtaaat cattcgatga
    26401 cgattcagaa aacgttcacc cgtaaaacga tcgctgacca gcacaacttt aaaatggctt
    26461 tcagagccgg ctgggacatt gtgacgatag ctttcatcca ctacttcgag gaatacgggt
    26521 tggaacgccg cccttaattt ttcttctatc cgctcacgta tcatcatgaa atatctcctc
    26581 cgacaacgct gagatgtcat ccatcccttt aaatactagc cgcttttacc gtttccattg
    26641 cagcttaaca acaaatattt aggtttcgtc ctggtttttc cgtcaagcgt atgaagttta
    26701 ctgatttaaa gattgaaaca caggcctcgc atcagtgttt cttttgccag gggcgattat
    26761 ttcgtcaaca tgatgaattt acatgcctta cccacttccc ctcgccgttg gcaatgttat
    26821 gatggcggga atttttacca aacacgctta agagtctctg agagcccgaa catgttcaaa
    26881 aaaatcctct tcccgttagt tgctctgttt atgcttgcag gatgcgcaaa accgccaaca
    26941 actattgaag tttccccgac gattacactg ccacagcagg atccaagcct gatgggcgtc
    27001 accgtaagca ttaatggtgc cgatcagcgt accgatcagg cgctggcaaa agtcacccgc
    27061 gataatcaaa tcgttaccct gaccgcctcc cgcgatctgc gtttcctgct gcaagaagtg
    27121 ctggaaaaac agatgaccgc gcgtggttac atggttggtc cgaatggccc ggttaatctg
    27181 caaatcatcg ttagccaact gtatgctgac gtgtcccagg gcaacgtgcg ctataacatc
    27241 gctaccaaag cggatatcgc catcatcgct accgcgcaga atggcaacaa aatgaccaaa
    27301 aactatcgtg ccagctacaa cgttgaaggt gcgttccagg cctccaacaa aaatatcgcc
    27361 gatgcggtta acagtgtgct gaccgatacc atcgctgata tgtctcagga caccagcatt
    27421 cacgaattta tcaagcagaa cgcgcgttaa tttctgccct ctggcccggt gcaaaccggg
    27481 cctgtagacg cccatgtcca gtcaatattt acgtattttt caacagccgc gttcagccat
    27541 attgctgatc ctgggttttg cttccgggct accgctcgcc ctgacatccg gtaccttaca
    27601 ggcctggatg acggtcgaga atattgatct caaaaccatt ggtttcttct ctctggtagg
    27661 ccaggcttac gtttttaaat tcctctggtc accgctgatg gaccgctaca cgcccccatt
    27721 ttttgggcgg cggcgcggtt ggctgctcgc cacgcaaatc ctgttattag tcgccattgc
    27781 ggcgatgggt tttctcgaac ctggcaccca actccgctgg atggcggcac tggcggtggt
    27841 gatcgctttt tgctcggcct cccaggatat tgtcttcgat gcgtggaaaa ccgatgtgct
    27901 cccggcagaa gaacgtggtg cgggcgcggc aatcagcgtg ctgggttacc gtttagggat
    27961 gctggtttcc ggcggcttag ccctgtggct ggcagataaa tggctgggct ggcagggcat
    28021 gtactggttg atggcggcgc tgttgatccc ttgtattatc gcgacgttgc ttgcaccaga
    28081 accgaccgac accattcctg tgccaaaaac gctggaacaa gcggttgttg cacctctgcg
    28141 agatttcttt ggtcgcaata atgcctggct tattttgctt cttatcgtgc tgtataagct
    28201 gggcgacgca ttcgccatga gcctgacaac cacgtttttg attcgcggcg tcgggtttga
    28261 tgcgggtgaa gtaggcgtgg ttaacaaaac gcttggctta ctggcgacca ttgtgggcgc
    28321 attgtacggt gggattttga tgcagcgcct gtcgctgttc cgggcgctgc tgattttcgg
    28381 cattttacaa ggtgcgtcta acgctggtta ctggctgctg tcgattactg ataagcatct
    28441 ctacagcatg ggcgcagccg tctttttcga aaacctctgt ggcgggatgg gcacatcagc
    28501 ctttgtcgcg ctgttaatga cgctgtgtaa taagtcattt tccgcaactc aatttgccct
    28561 gctctcagcg ctttccgctg taggacgagt ttatgtcggc cccgtggcgg gttggtttgt
    28621 tgaagcacac ggctggtcga cattctatct attctccgtc gccgctgccg taccggggct
    28681 tattttgctg ctggtttgcc gccagacgct tgaatataca cgagtaaatg acaactttat
    28741 ctcccgtacc gaatatccgg caggttatgc ctttgccatg tggacactgg cggcgggcgt
    28801 cagcctgttg gccgtgtggt tactgctgtt gacgatggac gcgctggatt tgacgcactt
    28861 ctctttcctg cctgctctgc tggaagtcgg ggttttagtc gccctttctg gcgtcgtgct
    28921 tggtggtttg ctggattatc tggcgctacg aaaaacgcat ctgacgtaat ctgtaaatat
    28981 tatttagctg tagtaatcac tcgccgtaat tattacggct aaataaacat cagtatcgct
    29041 taataatatt tgttcttttg tgcggcttag cgtttggaaa ttattgacgc aatttatgaa
    29101 ttctattttt tcttacacga ttcagctaat gagtctttat tttcccccca cccaattgtc
    29161 accataatga taattatttg ttaaacaatt gttttattcc agattggtta taccaattgc
    29221 ccgtccaaac tccttctgca ctcccctttc gttataacgc ccttttgcaa cagcttctta
    29281 aaatcaactt gatatgtttt gcaacatatg tgacctgaca gccaaatcca agtaacagga
    29341 atttaatcat gtttacagta atgtaacctt cccgtaaaat gcccacacac tttaaacgcc
    29401 accagatccc gtggaattga ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg
    29461 gatggttgtc attatttgca ggcactgtat tgctcagtgg ctgtaattct gcgctgttag
    29521 atcccaaagg acagattggt ctggagcaac gttcactgat actgacggca tttggcctga
    29581 tgttgattgt cgttattccc gcaatcttga tggctgttgg tttcgcctgg aagtatcgtg
    29641 cgagcaataa agatgctaag tacagcccga actggtcaca ctccaataaa gtggaagctg
    29701 tggtctggac ggtacctatc ttaatcatca tcttccttgc ggtactgacc tggaaaacca
    29761 ctcacgctct tgagcctagc aagccgctgg cacacgacga gaagcccatt accatcgaag
    29821 tggtttccat ggactggaaa tggttcttca tctacccgga acagggcatt gctaccgtga
    29881 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
    29941 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
    30001 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
    30061 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
    30121 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
    30181 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
    30241 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
    30301 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
    30361 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
    30421 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
    30481 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
    30541 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
    30601 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
    30661 cgggcgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
    30721 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
    30781 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
    30841 ttactgttgt tggtgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
    30901 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgtcg
    30961 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
    31021 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag atgccagtat
    31081 ttacctgggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
    31141 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
    31201 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
    31261 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
    31321 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt
    31381 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
    31441 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
    31501 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggacc atcggtttta
    31561 tcgtcacttt ctcggtgggc gggatgactg gcgtgctgct ggcagtaccg ggcgcggact
    31621 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
    31681 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagccttc ggtttcaaac
    31741 tgaatgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
    31801 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
    31861 acccgcagtt ccacaccatg ctgatgattg cagctagcgg tgcagtactg attgcgctgg
    31921 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
    31981 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
    32041 cgttctataa ctttgccgtt gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga
    32101 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
    32161 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
    32221 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
    32281 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
    32341 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
    32401 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
    32461 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
    32521 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
    32581 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
    32641 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
    32701 tttggtgccg gatttatcgg gatggaaatc tacgaattcc atcacctgat tgttaacggc
    32761 atgggtccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
    32821 ctgcacgtca cttccggtct tatctggatg gcggtgctga tggtacaaat cgctcgtcgc
    32881 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
    32941 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
    33001 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
    33061 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
    33121 cggccgtaat tctgggaaca atcctggcaa tggcagtagt acaggttctg gtgcatctgg
    33181 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
    33241 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
    33301 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
    33361 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
    33421 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
    33481 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
    33541 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
    33601 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
    33661 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
    33721 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat
    33781 tggctactgt gcagtaaccg gcgagttcga tagcggcgca gcgatcctgc tggctatctt
    33841 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
    33901 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
    33961 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
    34021 tggatataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
    34081 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
    34141 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
    34201 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
    34261 tttttattgt ctgagatcag agatacggct aataaccgta tgtcttgaca gagtgagcag
    34321 atgaacataa attaatcata cggttgatag ccgtattatg tatgagatcg actccctacg
    34381 atttcattca gttaatcaaa gaggtcataa tgcctggaaa aacagccact ctacccgatg
    34441 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
    34501 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
    34561 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
    34621 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
    34681 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
    34741 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
    34801 ggtaaccctg ttatcgataa atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc
    34861 gactgtgcgg ctaaagcact tattgacaga caaagtggtg aactgatcgg catctgtact
    34921 tttacggcat attcgctgga aaaacaacgc gtttctggcg tccttcaggg ttcacaacct
    34981 tcagaaattg gtgttgtcag attagtcatg ttgggggtag cacggaagta tcaaaagcag
    35041 ggctttggtc aggacctact atgtgatttt tttgaacatg taaaaataat tcaccaggca
    35101 ttaccaatta aaggggttta tcttgatgct gaccctgccg ccattaattt ttatgctcgt
    35161 ctcggctttg ttcagctttc agcgacacca aatgcttttg gtgctgtacc tatgtttttg
    35221 gcgattcagc atattctcgc ggcttagatt attatcgcca cccgccaggg caagttttgc
    35281 ctcagccggg ggctgagcag cgttaccact tttgttcaat tgcccccgct ttcagcaact
    35341 ccacatcctt gccgttaatg gttgccgttg cggcaacgat tgtggtcctt acagttgcac
    35401 aagattttcg ctcaaattcc ggtttataaa gaataagaga cgaattactg gtaaatgaac
    35461 caaataggta atcagtaaag ctaatattca gccggtttaa accgaaaggc gcgaagccaa
    35521 ccgtttggtt aatctcctta ccggatgggc gacgaatgct gaatgtcacg cgaagattgg
    35581 ccacttcgcc taatttattg gtggtggcga agaggctatc catgcaggaa aaacctttat
    35641 ctaaaaaatt atctcgcatc ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa
    35701 ccgtttttct gtcaatcatg tcttttcgac aagtagccag aaagtcttcc gggccaccat
    35761 aaatgtggac acctttatca agatattcct tcgctttctt acaatctacc tcaacatact
    35821 gaccattggc gtacataatg cccaacctgg accatgctaa attcacaccg tgctgcgcgg
    35881 cctgattaaa tagtgcatac gctttggcat aatccacccg cacctgctca ccgtcgtagt
    35941 agataccccc cagcgctaac ggagcttctc tgtcaccttg tttagccgct aactcgaacc
    36001 atttaatcgc ttcctgaaca tcaggtttgg tatcataacg aggctgataa tattcccagc
    36061 ctaacaggct ctgcgcgtcg atatctcccg attcagccat tttatgcagt gtatttataa
    36121 catcgggagc aggtttacca ccgtatatgc tgtcatacaa ttcatgcgct ttctcgctgg
    36181 ccaccacgct tggtgaaact atcagcatta cagaaaataa tattctttta gttaacatgt
    36241 atacctttat gtcctcactg tcatatattt ccataataat taaccaccaa atatccattt
    36301 aatgaataaa tacagtgcca taaaggtcca agtaataagc acacatttag cctgaaacat
    36361 tatcatccat gtcaccaaat cgtttattta gtgagataaa actagcatat tcgtaagata
    36421 aaaaatgcac atggcaatca agaaatgata ttattacttt gttgatgtaa aactatttag
    36481 ttttaaaatt cattttttca acatatagag cattcatttt atgaatgcaa aaaaacatac
    36541 ctgacacaca aattactctc ctctgtaata ttttctcatt aacactctgt ttacccaaca
    36601 tgcgctccgc actacactat gtgggcattt ttttaagggg tggtaatgaa cgattataaa
    36661 atgacgccag gtgagaggcg cgcgacctgg ggtttaggga ccgtattctc gttgcgcatg
    36721 ctgggcatgt ttatggttct gccggttctg accacgtacg gcatggctct gcaaggcgcc
    36781 agcgaagcat taatcggtat tgccattggt atttatggtc tgactcaggc cgtttttcag
    36841 attccgtttg gcctgctttc cgaccgcatt ggtcgcaaac cattaattgt cggtgggctg
    36901 gcggtgtttg ccgccggtag cgttatcgct gcgctttccg actccatctg gggaattatt
    36961 ctgggccggg cgctacaagg ctctggagca attgccgccg ccgttatggc gctgctttcc
    37021 gatctcacgc gcgaacaaaa ccgcaccaaa gcgatggcgt ttatcggcgt gagctttggc
    37081 attacctttg ccattgcgat ggtgcttggc ccgatcatca ctcacaaact tgggctgcac
    37141 gcgctgttct ggatgatcgc tattctggca acaaccggca ttgcgttgac catttgggtt
    37201 gtgcccaaca gtagcactca cgtacttaat cgtgagtccg gaatggtgaa aggcagtttc
    37261 agtaaagtgc tggcggaacc gcggctgcta aaactcaact ttggcattat gtgtctgcac
    37321 atgcttctga tgtctacgtt tgttgccctg cccggacagc tagctgatgc ggggttcccg
    37381 gcggctgaac actggaaggt ctatctggcg acgatgttaa tcgcctttgg ctcggtcgtg
    37441 cctttcatta tctacgctga agttaagcgc aaaatgaagc aagtctttgt cttctgcgtc
    37501 gggttgatcg tggttgcgga aattgtgttg tggaacgcac aaacgcagtt ctggcaactg
    37561 gtggtcggcg tgcagctttt ctttgtggcg tttaatttga tggaagccct tctgccctca
    37621 cttatcagta aagagtcgcc agcaggttac aaaggtacgg cgatgggtgt ctactccacc
    37681 agccagtttc ttggcgtggc gattggcggt tcgctgggcg gctggattga cggcatgttt
    37741 gacggtcagg gggtatttct cgctggcgca atgctggccg cagtgtggct ggcagtcgcc
    37801 agtaccatga aagaaccgcc gtatgtcagc agtttgcgca ttgaaatccc ggcggacatt
    37861 gccgcaaacg aagcgttaaa agtgcgtttg ctggaaactg aaggcgtcaa agaagtgttg
    37921 attgcagaag aagagcattc agcttatgtg aaaatcgaca gcaaagtgac aaatcgcttt
    37981 gaggttgaac aggcaattcg tcaggcataa aaaacgcggc gaattgcctg ctgcgctacg
    38041 cttaccaggc ctacaaagtt cttgagttac aaaccgttgt aggccgaatc aggcgttcat
    38101 gccgcatctg acatgaacaa aacgcacatt gtcgcgatta atcgcggaag tttttgaact
    38161 ggaacggctg gccgagatcg ccaccacgta ccatcgccat gacagcctgc aaatcatcac
    38221 gagatttgcc agtcacacgg atctcatcgc cctgaatttg cgcctgcact ttcagtttgc
    38281 tgtctttaat catcttgacg attttcttct gggtcgcgct ctcaatgccc tgtttcagtt
    38341 tcgcttccac aaaccaggtt ttaccgctat gaacgatatt ttccggtaca tccagtgaac
    38401 tgccttcaat gccgcgcttc agcagcttgg cacgcagaat atccagcaac tgattgacct
    38461 ggaagtcgga ctcgctcaac actttgatgg ttttgctggc gtcgttcagc tcaaatgagg
    38521 cttcaacgtt acggaagtca aaacgggact ccacttcgcg actcgcgtta tcgaccgcgt
    38581 tacgtgcttc ctgaagatca acttcagaga caatatcgaa agatggcatc ttttcctctc
    38641 ccttcatttt tgatgcgaag cataatacct gcaaagttaa ataaccaaca gtcctgcacc
    38701 aggacgtatc ccttaattgt gcatagtcgc aacagcatgc acaattaagg gatagcctta
    38761 ttgaacaccc ggagtggttg cgggtgagga ggaacaatga aaattaccgt attgggatgc
    38821 ggtgccttag ggcaattatg gcttacagca ctttgtaaac agggtcacga agttcagggc
    38881 tggctgcgcg taccgcaacc ttattgtagt gtgaatctgg ttgagactga tggttcgata
    38941 tttaacgaat cgctgaccgc caacgatccc gattttcttg ccaccagcga tctgctcctg
    39001 gtgacgctga aagcatggca ggtttacgat gccgtcaaaa gcctcgcgtc cacactgcct
    39061 gtaaccacgc caatactgtt aattcacaac ggcatgggca ccatcgaaga gttgcaaaac
    39121 attcagcagc cattactgat gggcaccacc acccatgccg cccgccgcga cggcaatgtc
    39181 attattcatg tggcaaacgg aatcacgcat attggtccgg cacggcaaca ggatggcgat
    39241 tacagttatc tggcggatat tttgcaaacc gtgttgcctg acgttgcctg gcataacaat
    39301 attcgcgccg agctgtggcg caagctggcc gtcaactgtg tgattaatcc actgaccgcc
    39361 atctggaatt gcccgaatgg tgaattacgt catcatccgc aagaaattat gcagatatgc
    39421 gaagaagtcg cggcagtgat cgagcgcgaa gggcatcata cttcagcaga agatttgcgt
    39481 gattacgtga tgcaggtgat tgatgccaca gcggaaaata tctcgtcgat gttgcaggat
    39541 atccgcgcgc tgcgccacac cgaaatcgac tatatcaatg gttttctctt acgccgcgcc
    39601 cgcgcgcatg ggattgccgt accggaaaac acccgcctgt ttgaaatggt aaaaagaaag
    39661 gagagtgaat atgagcgcat cggcactggt ttgcctcgcc cctggtagtg aagagactga
    39721 agccgtcacc actatcgatc tgctggttcg cggcggtatc aaagtcacca ctgccagcgt
    39781 cgccagcgat ggcaacctgg ccattacctg ctcgcgcggc gtgaagctgc tggcggatgc
    39841 gccgctggtc gaggtggcta atggcgaata tgacgtgatc gtactgcctg gtggcattaa
    39901 aggcgcagag tgttttcgcg atagtaccct gctggttgaa accgttaaac agttccaccg
    39961 ttccgggcgt atcgtcgcgg ctatttgcgc cgcgccagcc accgtgctgg taccgcacga
    40021 tatcttcccg attggcaata tgaccggctt cccgacgctg aaagacaaaa ttcccgccga
    40081 acaatggcag gacaagcgcg tcgtctggga tgcacgggta aaattgctga ccagccaggg
    40141 gccgggtacg gctattgatt ttggtctgaa aattatcgac ctgctggttg ggcgcgagaa
    40201 agcccatgaa gtggcatcac aactggtgat ggcggcaggg atttataatt attacgagta
    40261 gtgtcggatg cggcaaacgt cgcatctgac cagatgcgac gtaaaaacca caaattatgg
    40321 gcgatacacc ttcacattgt taaagccctg ctcgcgcaga tagagcgcct gcagacggct
    40381 catcaccccg cgctcacacc acagtagcca ggttctgttc tggtcgagat cgccaaactt
    40441 ggtgctcagt ttatagaacg gcagagaaac tacatcaatc ccttcgactt tcagtggctt
    40501 atcttcctgt tcatcgatag aacggatatc gaggatcacg tcgttcgggc cgaagccatt
    40561 gacggtttcc acttccacca cttcctgctc ggtctgctgg gcgatttcgc ggatatcaac
    40621 gttattcgct tcttcaacca ctttatcgag aatgctgaag tcgaatttct cttcttccgc
    40681 ttcaatcttc gacttaaccg ctttcaccgt cgggcttttg gagataacac cgcaatattc
    40741 cggcatcgtg cgagcaaagt cttcggtgcc aatctggcga gccaggttga tgatgtgctc
    40801 tttgtcgtaa gaaatcagcg gacgcaggat cagcgtatcg gagacgttat caatcaggcg
    40861 caggttggtc agcgtctgac tggacacctg gccgagcgct tcgccggtga ccagcgcctg
    40921 tacgccgtaa cgttcagcca ctttagacgc ggcacgcacc atcatacgtt tgaggataac
    40981 gcccatctga ccgtcgtcga ttttctcgag aatttccccg acgaccggtt cgaaattaat
    41041 agcgacaaaa cgcacgcggt gggagctgcc aaaacggttc cacagataat gcgccacctg
    41101 acgcacgcca atttcatgcg ccgcgccgcc aaggttaaag aaacagtaat gcacgcggca
    41161 gccgcgacgc atcaacatat aactggaaac accggagtcg aaaccaccgg aaatgagcga
    41221 cagcacatct tcctgggtgc cgatcgggaa accgccaata ccttcgtagc ggcctttaat
    41281 cagcaggaga cgatcgtctt ccacttccag atggacagtc acttccggat tggtcagctt
    41341 cacgcgcgcg gactcaatat gctgatttaa accgccgccg acgtaacgtt ccacatcaat
    41401 cgagctaaaa tcatgtttgc cacgacgctt cacgcgtacg cagaaggttt tgccttccag
    41461 ctgatcgcga tactgaacca acgctttctc aaaaatatcg tgcatgtcgg taaacggcac
    41521 gtcttcgact tcgagaatat ggtggatacc cggaatacgg gtcagggcgt cgcgaatagc
    41581 cagacgctgg ttttcatctt ttgcgcgaac ttcgatgtta tcccagtggc ggacgacagc
    41641 gagcgtctca tcatagtgct ttaaaacgtt acgaatgttc ccggtaagga tttttataaa
    41701 gcgcaagcgc acagattggc ttttgatggt gatttccggg aacaatttaa tgataaactt
    41761 catggcggca atggttcgtt ggcaagcctt aagcgacttg tatagggaaa aatacagcag
    41821 cccacacctg cggctgcatc caggcgcgga agtataccac taacatcgct ttgctgcgca
    41881 catcacctta ccattgcgcg ttatttgcta tttgccctga gtccgttacc atgacggggc
    41941 gaaaaatatt gagagtcaga cattcattat gccgaagaaa aatgaggctc ccgccagctt
    42001 tgaaaaggcg ctgagcgagc tggaacagat tgtaacccgt ctggaaagtg gcgacctgcc
    42061 gctggaagag gcgctgaacg agttcgaacg cggcgtgcag ctggcacgtc aggggcaggc
    42121 caaattacaa caagccgaac agcgcgtaca aattctgctg tctgacaatg aagacgcctc
    42181 tctaacccct tttacaccgg acaatgagta atggactttc cgcagcaact cgaagcctgc
    42241 gttaagcagg ccaaccaagc gctgagccgt tttatcgccc cactgccctt tcagaacact
    42301 cccgtggtcg aaaccatgca gtatggcgca ttattaggtg gtaagcgcct gcgacctttt
    42361 ctggtttatg ccaccggtca tatgtttggc gttagcacaa acacgctgga cgcacccgct
    42421 gccgccgtag agtgtatcca cgcttactca ttaattcatg atgatttacc ggcgatggat
    42481 gatgacgatc tgcgtcgcgg tttgccgacc tgccatgtga agtttggcga agcaaacgcg
    42541 attctcgctg gcgacgcttt acaaacgctg gcgttctcga ttctaagcga tgccaatatg
    42601 ccggaagtgt cggatcgcga cagaatttcg atgatttctg aactggcgag cgccagcggt
    42661 attgccggaa tgtgcggtgg tcaggcatta gatttagacg cggaaggcaa acacgtacct
    42721 ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt
    42781 cgccttggtg cattaagcgc cggagataaa gggcgtcgtg ctctgccagt actcgacaag
    42841 tacgcagaga gcatcggcct tgccttccag gttcaggatg acatcctgga tgtggtagga
    42901 gatactgcaa cgttgggaaa acgccagggt gccgaccagc aacttggtaa aagtacctac
    42961 cctgcacttc tgggtcttga gcaagcccgg aagaaagccc gggatctgat cgacgatgcc
    43021 cgccagtcgc tgaaacaact ggctgaacaa tcactcgata cctcggcact ggaagcgcta
    43081 gcggactaca tcatccagcg taataaataa acaataagta ttaataggcc cctgatgagt
    43141 tttgatattg ccaaataccc gaccctggca ctggtcgact ccacccagga gttacgactg
    43201 ttgccgaaag agagtttacc gaaactctgc gacgaactgc gccgctattt actcgacagc
    43261 gtgagccgtt ccagcgggca cttcgcctcc gggctgggca cggtcgaact gaccgtggcg
    43321 ctgcactatg tctacaacac cccgtttgac caattgattt gggatgtggg gcatcaggct
    43381 tatccgcata aaattttgac cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc
    43441 ggcctgcacc cgttcccgtg gcgcggcgaa agcgaatatg acgtattaag cgtcggtcat
    43501 tcatcaacct ccatcagtgc cggaattggt attgcggttg ctgccgagaa agaaggcaaa
    43561 aatcgccgca ccgtctgtgt cattggcgat ggcgcgatta ccgctggcat ggcgtttgaa
    43621 gcgatgaatc acgcgggcga tatccgtcct gatatgctgg tggtcctcaa cgacaatgaa
    43681 atgtcgattt ccgaaaatgt cggcgcgctc aataaccatc tggcacagct gctttccggt
    43741 aagctttact cttcgctgcg cgaaggcggg aaaaaagttt tctctggcgt tccgccaatt
    43801 aaagagctgc tcaaacgtac cgaagaacat attaaaggca tggtagtgcc tggcacgttg
    43861 tttgaagagc tgggctttaa ctacatcggc ccggttgacg gtcacgatgt gctggggctt
    43921 atcaccacgc tgaagaacat gcgcgacctg aaaggcccgc agttcctgca tatcatgacc
    43981 aaaaaaggtc gtggttatga accggcagaa aaagacccca tcactttcca cgccgtgcct
    44041 aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca
    44101 aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt
    44161 actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgt
    44221 tacttcgacg tggcaatcgc cgagcaacac gcggtgacct ttgccgccgg tctggcgatt
    44281 ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag
    44341 gtgctgcatg acgtggcgat tcaaaagctc ccggtcctgt tcgccatcga ccgcgcgggc
    44401 attgttggtg ctgacggtca aacccatcag ggcgcttttg acctctctta cctgcgctgc
    44461 ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat
    44521 accggctatc actataacga tggcccgtca gcggtgcgct acccgcgtgg caacgcggtc
    44581 ggcgtggaac tgacgccgct ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt
    44641 ggcgagaaac tggcgatcct taactttggt acgctgatgc cagaagcggc gaaagtcgcc
    44701 gaatcgctga acgccacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta
    44761 attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg
    44821 ggcggcgcag gcagcggcgt gaacgaagtg ctaatggccc atcgtaaacc agtacccgtg
    44881 ctgaacattg gcctgcctga cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc
    44941 gaactcggcc tcgatgccgc cggtatggaa gccaaaatca aggcctggct ggcataatcc
    45001 ctactccact cctgctatgc ttaacaaatt attcatagac tctaaataat tcgagttgca
    45061 ggaaggcggc aaacgagtga agccccagga gcttacataa gtaagtgact ggggtgaacg
    45121 aatgcagccg cagcacatgc aacttgaagt atgacgagta tggcaggagt ggcagcatgc
    45181 aatacaaccc cttaggaaaa accgaccttc gcgtttcccg actttgcctc ggctgtatga
    45241 cctttggcga gccagatcgt ggtaatcacg catggacact gccggaagag agtagccgtc
    45301 ccatcattaa acgcgcgctg gaaggcggca taaatttctt tgataccgcc aatagctatt
    45361 ccgatggcag cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag
    45421 acgtggtcgt tgcgacgaaa gtgttccatc gcgttggtga tttaccggaa ggattatccc
    45481 gtgcacaaat tttgcgctct atcgacgaca gcctgcgccg tctcggcatg gattatgtcg
    45541 atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg ctggaagccc
    45601 tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt
    45661 cgcagtttgc tcaggccctg aagctacaaa aacagcacgg ctgggcgcag tttatcagta
    45721 tgcaggatca ctacaatctg atttatcgcg aagaagagcg cgagatgctg cctctgtgtt
    45781 atcaggaggg cgtggcggtg attccgtgga gcccgctggc gcgggggcga ctgacgcgtc
    45841 cgtggggaga aactaccgca cgactggtgt ctgatgaggt ggggagaaat ctctataaag
    45901 aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt gaagaactcg
    45961 gtgcaacacg agcacaagtt gcgctggcct ggttgttgag taaaccgggc attgccgcac
    46021 cgattatcgg tacatcgcgg gaagaacagc ttgatgagct gctgaacgcg gtggatatca
    46081 ctttgaagcc agaacagatt gccgaactgg aaacgccgta taaaccgcat gcggtcgtag
    46141 gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc
    46201 acaactacga cagaataccc agcggccagt gatgaccgat aaagtacagg atgcctgcgg
    46261 aaatcacccc ggcgacaata tcgtcgatca tgatccccat gccgccatgt acattgcgat
    46321 caaaccagcg gatcggccac ggcttccaca tatccagaat acggaaaatc acaaacccgg
    46381 cggcaaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac ataccaataa
    46441 attcgtccca gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac
    46501 aaagatagac gccgatacag atccccaaca tcaccaccag cgagtagagc tgccagggta
    46561 aaaaggtcat cagataccag aacggaatcg ctgccagcga tcccatcgtg ccaggaacga
    46621 ttgggcttaa tccacttccg aatccgacag caagcagatg ccacggatta ctcatcttca
    46681 ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt catatccttt ccagtcaaac
    46741 gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt catttgcccg
    46801 atacaggtaa acggtacgcc caggtgtccg agagccacat ccagcgcgcc acggttcagt
    46861 tccggcacag tgaaacacaa ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc
    46921 tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatgc caggtcaata
    46981 cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag accgtcagag
    47041 agatcgatag ctgaatttgc cagatcgcgc agtgcctgtc cctgtaaaat acgcggcgat
    47101 ggacggagat gacgtttgat caagtagtcc gcatctttag catcggcaac ctgcaaacgg
    47161 ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg taccggtcac atagatccag
    47221 tcacccggtt tcgctccaga gcgcgttaag gctcgtccca tcggaacaaa gccgtggata
    47281 cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg catatcgtaa
    47341 taattgagaa gatcaaacaa actgtcgctg aaggactcaa gccacgcttc gtctacgtcc
    47401 ggtaaggtta atgccagcgt cagccaggcc ggatcggccc ccatcgctgc cagatcgctt
    47461 aggttcaccg ccagtgcttt atacgccaga tcagcaggat cgatatcatg gaggaagtgg
    47521 ttgcccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg gatattgaga
    47581 agagcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct tacacggtca
    47641 aaataacggg caatcaggga gaactcgcca catgccatac gttatgcctc agcaggtaaa
    47701 aaagaaaagg ccggcgatcg cggaacggtc ttccgtgaat ctaccggcct ggatatcact
    47761 ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg aacttatggc
    47821 tgtcttctgc gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc actttgtatg
    47881 gcacatcgct acgtttagac agctcgtaca gcgcaatgcg cagtactgct ttttctacct
    47941 gtcccagttc ttccagcagg cgggacaggt atggcttcat cagtccgtcg aggtatgcgg
    48001 tgttagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg tcttttacat
    48061 cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac aactgccagg
    48121 agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt ttcacggatt
    48181 tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc aagcgcggtc
    48241 agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat cgcttgttca
    48301 atgctttcag tggtcagaac cccaaaagca accggaattt cgctgtcctg ggcaacatgc
    48361 gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc accacgaata
    48421 accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc
    48481 agcggcagct cataggcacc aggcacccaa acaacggtaa tgttttcatc ttttacctga
    48541 ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat aaagttgttg
    48601 aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc ttcaataatg
    48661 ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt tagcataata
    48721 tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat gcaggcaaac
    48781 atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc
    48841 aagccctggc agcgagcata atccacgggc gtcgctgcct aatagtttag gtgcgatata
    48901 gacaatcagc tcatcgacta aacccacctg cagcaatgcg ccagcgagcg ttggccccgc
    48961 ttccacccag atgctgttaa tttgctgttt acccagttgc atcatcagta caaccagatc
    49021 cagatgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc actcacgaga
    49081 atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc gatgttccgg
    49141 cgtcacgcga ttttggctat caatcacaat acgtaccggc tgacggagat tttgttgcgg
    49201 gtagagcacc tgagtttgtt catccagttc ggaccaacgc accgttaagg caggatcatc
    49261 cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgcgcgcgca gtcgttgtac
    49321 gtcgcgccgc gcctgagttg aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg
    49381 gccatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgctt
    49441 gagaaagcct ttattcaatt gctcggcttc actcatcatc agcccgtggc tgacgtcaat
    49501 gccagcctgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag gatcttgcat
    49561 cgctgcgacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg
    49621 accatgatgg ctacagggtt cgagtgtgac ataggcggtc gcacctttgg ctttttcacc
    49681 cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gctcgttggt ggtaaccttc
    49741 accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt
    49801 aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt cctgcacggc
    49861 ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga aactgcggta
    49921 gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct ccatcaccag
    49981 attgccaatc atcttgctcg gtacttcgcg ctcaccggtg gcgcgcaact gcgatttaat
    50041 atgattgatt gccatttcaa cgtcatcgga actcaccgga cgtttttcca gcgcccgcag
    50101 cattccgctg cgcaatttct cttcattaaa cggttcacgc acgtcgttgc tttttacaac
    50161 acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac acaccagaca
    50221 ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa ttaccttagt
    50281 gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg ttaacggaaa
    50341 tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga taaaggatcg
    50401 atgctttcgc tgcttttacc ctctaccatt aggctaatta cgacgagaaa gggacgacct
    50461 atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct tagtgcctgt
    50521 gtgcagcaaa gcgaagtgcg acagatgaaa cacagcgtca gcgcgctgaa ccaggagatg
    50581 acgcaactca atcaagaaac agtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc
    50641 agcagtgggg tttaccttct gcctggagcg aaaacaccgg caagactgaa aagccagatc
    50701 ggtactttac gtatgtcgct ggtgaatatt acgcctgata cagatggcac caccctgacg
    50761 ctacgtattc agggtgagtc gaatgatccc ttgcccgcat tcagcggaac tgttgaatat
    50821 gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcaaaa tcaattgatt
    50881 aacgcccctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca gttaaagggg
    50941 atttctgtgg atcagttagg ctttgtacgt atccacgaca ttcaacctgt tatgcagtaa
    51001 acgtatttcg gggcgatttt gtgcgtcccg caacatcttt ccccgtcatt ttgttactct
    51061 gcttacatca cccagattga tagtaaaagt ttgcaacaag ggtgaaagtc agtacaatcc
    51121 ctgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac
    51181 gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta
    51241 gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat
    51301 gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa
    51361 cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt
    51421 tgtcggaagc tatcacaccc gtttcggacc gcagatccgc aatgatacct accttgagta
    51481 cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt
    51541 cttcggcggt aactccgatg caaaaggtat ctggaaccac ggttctccgc tgtttatgga
    51601 aatcgaacca cgtttctcca tcgacaagct gaccaatact gaccttagct tcggtccgtt
    51661 caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggtcg
    51721 ccagagcacc tggtacatgg gtctgggtac cgacatcgat actggtctgc cgatgagcct
    51781 gtccatgaac gtctatgcga aataccagtg gcagaactat ggcgcagcga acgaaaacga
    51841 gtgggacggt taccgtttca aaattaaata ctttgtgccg attaccgatc tgtggggcgg
    51901 tcagctgagc tacatcggct tcaccaactt cgactggggt tccgatttgg gggatgacag
    51961 cggtaacgca atcaacggta ttaagacccg tactaataac tctatcgctt ccagccatat
    52021 tctggctctg aactacgatc actggcacta ctctgtcgta gctcgttact ggcacgacgg
    52081 tggtcagtgg aacgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc
    52141 taccggctgg ggtggttacc tggttgtagg ttacaacttc tgattatgaa aatgccggga
    52201 tttattcccg gcatttcaga ttgttaacaa cgtgcgcttt gttcatgccg gatgcggcgt
    52261 aaacgctttg tccgacccac aaaagcacgt aaattcaata tattgctgaa ataacgtagt
    52321 cctgataagc gtagcgcatc aggcaatttt acgttttaat cacttcttct tgttcatcat
    52381 cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc
    52441 ccctgcgata acggtcgtac catactgatc cgcttcggta tatttcgaat gctcatgatc
    52501 gtggcaatag agacaaagca attcccagtt actgccatct tccgggttat tggtatggtc
    52561 gtgatcaatg tggtgaacag taagttcacg caggttggaa tagacaaact cgcgggaaca
    52621 gcgaccgcag acccacggat agatttttaa tgctttttcg cgatagccgc tttctaaccg
    52681 cgcatagttt tttgggatga tagccatgaa aagttacctg gatgaaatgt agggttacgc
    52741 gcagtttccc aagatgcggg gattttcgca atctccatac atcaaaaaaa atcccgatct
    52801 tctgaccggg attttcaaca tcaacgggaa cttgattaag gcagaattga cggctgatcc
    52861 gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga
    52921 gccaacgcag atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg
    52981 gtcagcgaga agccttccag taccggacca ccgaagaggt acagcatcag gataaccatc
    53041 aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag
    53101 atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaatacg gtccgatacc
    53161 acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg
    53221 tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc
    53281 gcaataacca cccctgccgc cagtcgccac tcaaagcgga aacctacgta cacgaggata
    53341 gacagcagcg ctgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc
    53401 ggaccgacga actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc
    53461 ttcagaacct ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg
    53521 accatgatgt cgtggctgct accaaagttt tgcagcattg gctcttcaaa accggctttt
    53581 tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc
    53641 gtaccaccgg taaaatccag cccccagtta aagccgcgca cgcccataat aacgatagca
    53701 gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag
    53761 actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca
    53821 gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc
    53881 gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt
    53941 aattgccccg gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc
    54001 atcgaagata gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg
    54061 tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgccac
    54121 cgcaagggtt aagacgatac ccgcgatacc tggcatactc agcgttgcgc ctggcaacag
    54181 cgacataatg ccgacgatta agatcaagtt ggcaatcaga gcactggtcg caatcagacc
    54241 aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc
    54301 ctccagcccc tgttcaatgt tctgcatacc cagggttggg ccaatggtac gttcttcaac
    54361 aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc
    54421 gttcgggttg ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc
    54481 gatgttaatc acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc
    54541 gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt ccttagtgaa
    54601 gttagacatg atgttaccac cagcgctatc gagcgagatg ttaacctgcg gttggttgta
    54661 ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg gtcagaatta cgcgtttgta
    54721 cagcacaact ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc
    54781 ggatgccgca gcggcctggt caacgttggt gtttaccaga cggaattcca gcgttgccgt
    54841 cgcacccaga atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat
    54901 acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt
    54961 acgcaggata ttaatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc
    55021 gctcattacc gcacgcagct ggttgctgcc ctggctgcta atcaccaggt ccggatggcg
    55081 cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag taatgctcag
    55141 gccgtagttg ttttctttac gaacggtggt atacgggatg cccttttcac gcagatcgct
    55201 gcgcaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc
    55261 catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcgtgaat
    55321 agctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc
    55381 cataacgccc attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc
    55441 aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt
    55501 tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg
    55561 aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac
    55621 cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg
    55681 gaaaaatttt aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc
    55741 acgtttaata actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc
    55801 tactttggtt acgcggccaa ccaggccacc gttcgtcaga acttcatcac ctttggcaat
    55861 ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa
    55921 atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc
    55981 cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt
    56041 attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag
    56101 ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct
    56161 ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt
    56221 caagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat
    56281 cgagtgggcc agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca
    56341 cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac
    56401 cacgacgtac gccttcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt
    56461 tgtctgccgg aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct
    56521 cacccacagc cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa
    56581 cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat gataccaaac agcgcatttt
    56641 tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca
    56701 tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga
    56761 cgatatccga accaagatcg tactgaatct ccattgattt ttcaggatcg aggaaaatcg
    56821 gatcgccgtt gatcgggtta cggaagtgca cgccctgttc ggtgatttta cgaatatcgc
    56881 caaggctgaa gacctggaaa ccgccggagt cggtgaggat cggccccttc cactgcataa
    56941 aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga
    57001 aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt
    57061 ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa
    57121 agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt
    57181 ctccactacg tcagaaaaac agtccaacgt tttaaaccag cgccgcggaa ttactccccg
    57241 acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa
    57301 aagcgatatt tctcttctac cgctgcttta taggcgttca tggtgtgttg ataaccggca
    57361 aaggccgaaa ccagcataat cagcgtcgac tctggcaagt ggaagttcgt caccagcgca
    57421 tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc gaagaacggt
    57481 tcaatgagat cgtttttcgc tgcctgagcc gcgctttcca gcgaacgtac tgaagtggtg
    57541 ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc
    57601 tgcggtactt cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgcacc
    57661 ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg
    57721 cgcaattttt ccagcaaagg ctcgtcaaaa tgcagaccag cggtcggggc tgcaaccgcg
    57781 cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacgg
    57841 tcgatatacg gcggcagcgg catatggccg atactgttga ggatatccag taccgaacga
    57901 tcatcattga attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata
    57961 ctttcgtcat cgcccagcag cagttctgcg ccaggttttg gcgctttcga ggcgcgaata
    58021 tgcgcaagaa tgcgtttgtc gtcgagcatc cgctcaacca gcacttcaat cttgccgccg
    58081 ctggctttac gcccaaacag gcgcgccggg atcacgcggg tattattaaa aaccagaaga
    58141 tcgccggggt tgagcttatc aagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc
    58201 ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc
    58261 agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa
    58321 ataagaggcg ggtagtctag tgccggggcg ctctccctgc aacctttacc cccttttgga
    58381 tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc
    58441 gctttccggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc
    58501 cgacgtcgtg gctggcattc atatgcatcg acgtatcgac gtattaaccg acaatctgcc
    58561 ggaagtccgc gaagcacggg aatggtttcg tagtgaaact cgccgcgttg cgcctattac
    58621 gctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt
    58681 tccgctacag gaatttgtct gttatgcccg cgagcaagtg atgacgattt tgccggactc
    58741 accgccacgt tttatcaatc tgaacaatta cttgtggtca gagcagtggc tggtgcgcta
    58801 tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc gcccacgtct
    58861 ggatgccctg cgtgactcct ggtacgattt agacgctcat tatgacgccc tcgaaacccg
    58921 cttctggcag ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt tataaaacgt
    58981 tagttcatcc ataccgtagc cgaaatggca ggcaaagcca gaatcccgtt tgtcagttgc
    59041 ccatggcctt ctttgcgttg ccattgcgcg acattaagaa acggtgacgc ggggagcacc
    59101 acttcacagg cctcgccacg gttgattgcc accagtacac gttgctgatt cagcacgcgg
    59161 acaaatacca ctacgttatc ttccgcatac agcacctgac agccgccacg acgcagcgcc
    59221 tgacttttct tacgcagcgc aatcattcgc tggtacagcg cgaataacgc cgtatcctgc
    59281 ttttccacct gccaggggaa cggtttacgg caaaacggat cgtttttgcc atccagtcct
    59341 acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca gaccaccgcc
    59401 agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt atcgtggctg
    59461 tcgagctgat taaacatacg taattgttgt tgatgagaaa gccctgcgcg gtaattatcc
    59521 atccaggcca tacaggtttg ggcatcaatt tgctgcggat cgtaagagat atcggtattg
    59581 gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt tcatggcggc atcttccaca
    59641 tcggcctgta accattgccg tgcatcgcca aaatgttcgc cgacaatata cgcttccggc
    59701 tgggtttctt tcgccgcttc ggtgatccca gcaacgtgct gcatattatt gcgcgcccca
    59761 cccgcctccc ccagcatatg caccacatcc agccgccagc cgtccatact ccacggcgct
    59821 ttcagccaat ggcggacaat actgtcttcc ccgcgataaa tttcattcac cagactttcc
    59881 gactgataat ccagcttcgg caagctggca tagccaagcc agtcgagcgc cgtgccatca
    59941 tcactaaacg agtaccagtc gcgccagggc gattcagggt tgtgacaagc tccccccgtg
    60001 ccacgattgt gcctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa cacgccatcc
    60061 agcaccagcc gcattcccag ctgctgcgta ttgtggcgta agcgcagcaa cgccccatca
    60121 ccgccaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg tacgctggga
    60181 gctttaaaca ccggattgag atacagcgcc gtcacgccaa gctttttcag atacggcagt
    60241 ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc ctgcgccgtg
    60301 accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg gtaatagaca
    60361 tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa gatctgataa
    60421 aaaatctgat ccgcagccca ttgtgggccg atatccggta catcgacggc aaactgctcc
    60481 agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg gcgatcgtgc
    60541 cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc aatcgccgca
    60601 cgccatgcgg tgacgccagg ctgctgctga ctgcgctgct tatgcatcga tactgacatt
    60661 tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc gcccgtcagc
    60721 cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac cggcaggtgc
    60781 catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc catagcctaa
    60841 tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc ggataacttt
    60901 cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag gattactggt
    60961 tttcagccag ctgacgatcg tggcggcgtt taaacatcca gccaatcaac agcacaacaa
    61021 tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca atcaacccga
    61081 taataaagag caggaaaatc aaaccgccga tggtcgttgc taccccaccc ggcactttaa
    61141 atttcagcgc cttaacttct tctggcggca aacggcgacg gaaggcaatt tgcgacagca
    61201 ggatcataat ccacacccac accgtggcga aggttgccag cgaagcgatc accaggaaga
    61261 cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta gtcatcacca
    61321 gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc ggcgcgctgc
    61381 cctgctctgc cataccgtgg agcatacggc ctacgccaaa tacgtcactg ttaattgccg
    61441 acagcgaagc agtcagcaca acaaagttaa gaatgctggc ggcaaaggta atgcccatat
    61501 gctggaacgt cagcacgaac gggctaccgg cagtgccaac ctgattccac gggtagatag
    61561 acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc acggagttaa
    61621 tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc ggcggtaatc ccgataattt
    61681 cgatcccacc gtaagcaaac atcaccattt gcaacgacat caccatccca agccagccgt
    61741 tactgaagaa gccgccgttg ctccacaggt tatgaatacc ggtcggttgc ccgccgttgc
    61801 caatccccca gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg gtggcgactt
    61861 taaagaacga gaaccagaat tccagctcac cgaagacctt cacgctcatc aggtttacgg
    61921 cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc gggaaccaga
    61981 cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg atttcaaagc
    62041 aataggtcca gccggtaatg taacctgcca gcgggccgag gttttcctgc gcataacgcg
    62101 agaaagagct ggctgccggg ttatgtaccg acatttcccc cagcgcacgc ataatgatat
    62161 acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc attttgatgg
    62221 cgtctgccga accgtaaaac agcccggtgc caattgctga acccagtgcc ataaagcgta
    62281 tgtggcgggt acttagccca cgctttagct tgttcttact ttccatcaat tcctaaccca
    62341 tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa tgttcagtga
    62401 tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata atggccagaa
    62461 ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc ggtaaacgct
    62521 gcgcccagga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg agaataccaa
    62581 aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc caccatgagc
    62641 gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc agcaccggta
    62701 cggagatctg gatcagctgg cttaagccga ggttagaaac caccatcgag aagccgccga
    62761 ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat tctgcacaag
    62821 cacaggtcag gccaactgcc gttaccaggc aggcgatgaa gattaacgcc gccagcagga
    62881 agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca gcgccgtttg
    62941 cagactgatc gaccagcgac gcgctgtctg aacccagacg gaacagcgcc aggtacagca
    63001 gagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc agcagacgcg
    63061 cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac accattgcgc
    63121 ccagcgtatc catggtcaga tagccgttaa cgaagccgtt agaaaacgca gcgttttgat
    63181 aagcctcagt cgccgtgctg atagaacccg ccggccagac aatcgcggca acagacagga
    63241 tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc agcagcttgc
    63301 ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag ataaacagcg
    63361 gcagcgcgga atcaccagtc agcggcgcaa tccccacttc aaaggaaacg gtagctgtac
    63421 gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc agtacgccag
    63481 cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt gccagcgcca
    63541 ctaccgttag taccggcaga ccaacggcag taatgaggaa gccgaatgcc gcagtccaga
    63601 cgtgttcgcc tgcctgcaag ccgaccattg gagggaaaat aatgttacct gcgccgacga
    63661 acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat tgatgggtca
    63721 taaatcatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc aatccctggg
    63781 gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc aggcgcggcg
    63841 aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc tgaagcgcaa
    63901 tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg tatatagcga
    63961 taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg ctaatcatgc
    64021 gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt attttgactg caaagaaaat
    64081 aagccagcat cggctggctt atggcaataa aagatgacaa aggcggatta atcgctgttt
    64141 ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc tactgtactc
    64201 tcaatattca ggcgactttc gtgatgattc accgcatgtt tcacgatcgc taaccctaat
    64261 ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg ctcggtcagg
    64321 cgcggaatat gctccggtgc aatgcccggt ccgttatctt caacgctaaa ttcggcaccg
    64381 tgcggcactc gctgccagcg tacggtgata tgcgtgcctt ctggagtatg attcacggcg
    64441 ttatagacca ggttcgaaat cgcactgcgt agctgatctt cgttgccaga cactttgagg
    64501 ccgttatcta tctcaaaggt aaatgtctgt tttttctgac tcagagtctg agcctcacgc
    64561 tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaaatg cgtcggtgcg
    64621 gcttctattt tcgacagcgt cagcaactgc ttcaccagcc cttccatccg ctgggtctgc
    64681 tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg ctcatccatc
    64741 atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct cacgttggca
    64801 aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg cgccaccatc
    64861 agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc ggtgttgagc
    64921 accagattga gcgggcgaga aaaatcacgc gttttcagat attgcgtaaa ctccgggtaa
    64981 cgcagcaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc aagaatttgt
    65041 tgcgccagac cgttacacca gaaaataccg ccctcttcag tggtcagcac cactgcgtcg
    65101 ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc acggcggcgt
    65161 tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca gctaccacgc
    65221 cccggtggcg gggtcatact acgatccacc cacagccacc atgaaaggcg caataaattc
    65281 cagaaatgcc agataagcag tcccgttacc gatgccagca aaaaccaggg caggtaacca
    65341 aaaaatgcac ccaggatgaa agccgggaag cagcaaagta gcagctccag caccagcctt
    65401 ttccacgaca gccgttccag cacgtaagat actccagtta agaaatcata agccctgctc
    65461 tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt accgcgcacg
    65521 gtctgcacca tgcggtcatg cccgcctggc tccagtgctt tacgtaggcg acgaatgtgg
    65581 acatcgaccg tacgatcttc cacatacacg ttagttcccc agacgtggtt taacagctgc
    65641 tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt aaattctgtc
    65701 ggccccatct ccagcggctc ttcgcccgcc atcactcggt gagatgtcgg gtcgagactt
    65761 aatccctgca tctcaatcac ctcttccacc gccattggcg aaatacggcg cattaccgct
    65821 ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg tgatatagtc atccgcgccg
    65881 gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat caccactgga
    65941 atatcccggg tcatcgactc gcgcttgagg tgtttgatga actggatacc ggagccgcca
    66001 ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg attcacagca
    66061 ctgtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac gaagcagacc
    66121 atttcgcgaa ttggagcttc atcttctacg accagaatac gtctcgccat gatttgccct
    66181 gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga tttatgacag
    66241 atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc gcagaaaaaa
    66301 gcaaatggca catctgtttg ggtataatcg cgcccatgct ttttcgccag ggaatcgtta
    66361 tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt aaaagccgcg
    66421 aagctgaaca tcaggctttt cttgactggc tgctggaaac agcacaaacc catcaggtgg
    66481 atgcgattat tgttgctggt gatgttttcg ataccggctc gccgcccagt tacgcccgca
    66541 cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg gtggtactgg
    66601 caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg gcgttcctca
    66661 atacgaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt cgcgacggga
    66721 cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc gcgtgacatt attaccagcc
    66781 aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt accgattatt
    66841 accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg cccatcatcg
    66901 ccacgggaca cttaacgacc gtgggtgcca gtaaaagtga cgccgtgcgt gacatttata
    66961 ttggcacgct ggacgcgttt ccagcacaaa actttccacc agccgactac atcgcgctcg
    67021 ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat tgcggctctc
    67081 ccattccact gagttttgat gaatgcggaa agagtaaata tgtccatctg gtgacatttt
    67141 caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa cccatggcgg
    67201 tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc gatgtatcac
    67261 aggagccgcc tgtctggctg gatatcgaaa tcactgctga tgagtatctg catgatattc
    67321 agcgcaaaat ccaggcatta accgaatcat tgcctgttga agtattgctg gtacgccgga
    67381 gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc agcgaactca
    67441 gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga actggatgaa tcgcagcagc
    67501 aacgtctgca gcatcttttc accacgacgt tgcataccct cgccggagaa cacgaagcat
    67561 gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat ggaagattga
    67621 tttcacccgc gagccgttcg ccagcaacgg gctgtttgcc attaccggcc caaccggtgc
    67681 ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcacgaaa ctccgcgtct
    67741 ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgctg aatgtctggc
    67801 ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca ttctggagcc agaatcgggc
    67861 gcgtaaccaa cccgacggta atttgcaggt gccacgcgtt gagctggcgc gctgcgccga
    67921 cggcaaaatt ctcgccgaca aagtgaaaga taagctagaa ctgacagcaa cgttaaccgg
    67981 gctggattac gggcgcttca cccgttcgat gctgctttcg caggggcaat ttgctgcctt
    68041 cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg gcactgaaat
    68101 ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca ctgagctgga
    68161 gaagctgcaa gcgcaggcca gcggcgtcgc gttgctcacg ccggaacaag tgcaatcgct
    68221 gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttacttaccg cgcagcagca
    68281 agaacaacaa tcactaaact ggttaacgcg tctggacgaa ttgcagcaag aagccagccg
    68341 ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac ctcaactggc
    68401 ggcgctcagt ctggcacaac cggcacgaaa tcttcgtccg cactgggaac gcatcgcaga
    68461 acacagcgcg gcgctggcgc atactcgcca gcagattgaa gaagtaaata ctcgcttaca
    68521 gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg gcgaagcagt cagcagaatt
    68581 acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct tccgtcagtg
    68641 gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg atcgcgagca
    68701 tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaatg cgcttgcggc
    68761 gatcacgttg acgttaaccg ccgatgaagt tgctaccgcc ctggcgcaac atgctgagca
    68821 acgcccactg cgtcagcacc tggtcgcgct acatggacag attgttcccc aacaaaaacg
    68881 tctggcgcag ttacaggtcg ctatccagaa tgtcacgcaa gaacagacgc aacgtaacgc
    68941 cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg ccgatgtgaa
    69001 aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gctcaacgtg cacagttaca
    69061 ggcgggtcag ccttgtccac tttgtggttc caccagccac ccggcggtcg aggcgtatca
    69121 ggcgctggag cctggtgtta atcagtctcg attactggcg ctggaaaacg aagttaaaaa
    69181 gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggac gccttaacaa agcagcttca
    69241 gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag caagcactta ctcaacaatg
    69301 gcaagccgtc acggccagcc tcaatatcac cttgcagcca caggacgata ttcaaccgtg
    69361 gctggatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac gacatgaatt
    69421 acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc aaattgaaca
    69481 acgccagcag cagctcttaa cggcattggc gggttatgca ctgacattgc cacaggaaga
    69541 tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc agcaacgcca
    69601 gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acgccgattc tggaaacgtt
    69661 gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata actggcggca
    69721 ggttcatgaa caatgtctcg cattacacag ccagcagcag acgttacagc aacaggatgt
    69781 tctggcggcg caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc tacaggccag
    69841 cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa cactaacgca
    69901 gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa cgctggtcac
    69961 tcagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt tggctctcac
    70021 tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt tgcgtgaaaa
    70081 caccacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata accgtcagca
    70141 acaacaaacc ttaatgcagc aaattgctca aatgacgcag caggttgagg actggggata
    70201 tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg cccaggggct
    70261 gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc acgggcgcta
    70321 tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata cctggcaggc
    70381 agatgcggta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg ttagtctggc
    70441 gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact cgctgttcct
    70501 tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg atgcgctgga
    70561 tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgtagaag cgatgaaaga
    70621 gcgtattccg gtgcagatca aagtgaaaaa gatcaacggc ctgggctaca gcaaactgga
    70681 aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg ggcgaattat
    70741 ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac ggtgattggt
    70801 agccgttctg gtggttgtga tggtggtatg aaaaaagtca ttttatcttt ggctctgggc
    70861 acgtttggtt tggggatggc cgaatttggc attatgggcg tgctcacgga gctggcgcat
    70921 aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc actgggggtg
    70981 gtggtcggtg cgccaatcat cgcactcttt tccagccgct actcactcaa acatatcttg
    71041 ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc ttcgtcttac
    71101 ctgatgcttg ccattggtcg cctggtttcc ggctttccgc atggcgcatt ttttggcgtc
    71161 ggcgcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc cgtggcgggg
    71221 atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac gtatttaagt
    71281 caggaattta gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg
    71341 gcatcggtct atttttgggt gccggatatt cgcgacgagg cgaaaggaaa gctgcgcgaa
    71401 caatttcact ttttacgcag cccggccccg tggttaattt tcgccgccac gatgtttggc
    71461 aacgcaggtg tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt
    71521 ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat ggtgctgggg
    71581 aatgtgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtaact
    71641 gactttatca ttgtactggc actgctgatg ctttttttct gcggcggcat gaaaacaacg
    71701 tcgcttattt ttgcttttat ttgttgcgcg ggattatttg ccctttcagc acctctgcaa
    71761 atattgttac tacaaaacgc caaaggcgga gagttattag gtgccgcagg tgggcaaata
    71821 gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg
    71881 ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc gatgtcatcg
    71941 ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa
    72001 ccactggggt agattactgt cacggtgcgc gttgcttatt gccggatgcg gcgtgaacgc
    72061 cttatccgcc ctacgcggtt ccggcacatt ttgtagatac tgataagacg cggcaagcgt
    72121 cgcatcaagc atgatgcgcc aattgcctac gttttttact cttgtggcca taaccacgca
    72181 gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg
    72241 ccgccgaaga caaattgttt aatcaactgc ccaaccgttt gatataaacg gtctacattg
    72301 ctcatcccgc cccccaggac aatcacatcc ggatcgagaa tattcacgac atgtgccagc
    72361 gattttgcca gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg atcgctttct
    72421 tcaaccaggc ggataatttc actgcctttc agcgcatgtc cgctcaaacg acgataatct
    72481 gtggcaaatc ccgtgcccga aataaaggtt tcaatacaac cttgtttacc gcaataacaa
    72541 gggacttcct cgcgataacg cagttcgtct tcgtccatcc acggtagcgg attgtgtccc
    72601 cactcgcctg ccgtgccatt gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg
    72661 ccgcatcccg tgccgataat cacggcaaat accgtctgtg ctcccgctgc cgcgccatct
    72721 actgcttctg aaacggccag acagttggcg tcatttgcca gccgcacttc ccgctgcaac
    72781 ctcgcgctta agtctttatc gaatggctga ccgttgagcc aggttgaatt ggcattctta
    72841 accacaccgg tgtaaggcga aattgagcca ggaatgccca tacctaccgt tccgcgctgc
    72901 cctgtcgcct gctccgccat atcaaccaac gtggcgatcg tttcaatagt ctgccggtaa
    72961 tcatcacgcg gcgtgggcag acgatggcgg tacaactgct cccctgcatc gcccagtgca
    73021 atcacttcag ttttggtgcc gcctaaatcg atacctatac gcacggtact ctccttattt
    73081 ttttcaatat caatagcgta gagacggaca accggattgg caatgcaagg ccgccgacaa
    73141 ttcgttatca tgcccgctaa atttaacgac aaggccgtgg aaattatcat gctgtggttc
    73201 aaaaatttaa tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga agagatggaa
    73261 aaacagctag cctcgatggc atttacccca tgcggcagcc aggacatggc gaagatgggc
    73321 tgggttcctc cgatgggatc gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt
    73381 gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa gcaggcgctg
    73441 gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa
    73501 gattcgctga aagacgaagt gctgcactct ctgttgccgc gtgctttcag ccgttttagc
    73561 cagacaatga tgtggatcga cacggttaac ggtttgatta tggtggactg cgccagtgcc
    73621 aaaaaagcgg aagatacgct ggcactgctg cgtaaaagcc tggggtcgtt accggttgta
    73681 ccattgagca tggaaaaccc gattgaactg acgttgaccg aatgggttcg ctccggtagt
    73741 gcggcacagg gcttccagct gcttgatgaa gccgagctga aatcgttgct ggaagatggc
    73801 ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag agatcaccaa tcacattgaa
    73861 gccggaaaag tggtgactaa actggcgctc gactggcagc agcgcattca gtttgtgatg
    73921 tgcgacgatg gttcgctcaa gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa
    73981 gatatcgacc gtgaagattt cgcccagcgt tttgatgccg atttcatcct gatgactggt
    74041 gaactggcag cgttaattca aaacctgatt gaaggattag gtggcgaagc acaacgttaa
    74101 ttgctgattt tcctttaatg ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag
    74161 ccacaccagg catataatta ttcgctacgg cgggcaataa tttttagcgc agcaatatta
    74221 tgcgttttat gctgtaactt gctccatgga cgttgtgtca ttgtttttcc tcaagccagt
    74281 gacgcgcttc actgcgaatt ttccgcattg ccccggtttt cactaaatcc cgtaacagcg
    74341 aaagatcggg ttgttcgaca aataagagta atgatggatc ctcttttaac cacgtcgttt
    74401 tcacatcggc agcgagctgt ggattattca tagctagcga ccgatgccga gactcggtca
    74461 atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat
    74521 cattgcgact ttgcagcaat gcacgccacc attcatcatc tgatttatct tttatcgtag
    74581 ccagttccgc aacaatatct ggagatcttc actgatattt atatcaacct tgatgaatta
    74641 tgaatatacc ggaaattgaa aaaatcgcag caaaggaaat attattatgg gttaaagaga
    74701 ggatatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac agatagcggc
    74761 acagataaga ggtgggttcg gcaacttgca gatgaaactc actgtgaccg ggaacattaa
    74821 acaccgaacc ggcttcatac acctgccagt cggtcgcgtc aggcagtaac acattcagcg
    74881 cgccactgat taccgtcatt tcttccggct cagcggtgct gaaggtgtat tcgccttcaa
    74941 ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatt gatttcactt
    75001 tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtttggta gttgaacatc
    75061 tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacacca gtaattccgc
    75121 agccagccgt gcaatcaata cgttagacag caagacagga acatcgagct gtttttgcag
    75181 taaatcgcga tgacgttggt gaaatcctaa acaatccagc atgatgacat cagccccttt
    75241 tgccagtaat tctttcccgg catcaatgat tttttgttct gaatcatgaa tggggttacc
    75301 caatgaaaat accggcgatt tctgcaaaat ttgccatttt tgcgcctgaa cgggcagcat
    75361 ctcctcaacc ggaacgataa cccccacctg atgatcttca acaatagagg aaaccagtgg
    75421 aggcaatatt cgcgacggct caagaaagat cgtattacgc gcagtcatac tactaatgtt
    75481 tgctgtactc attaataaaa tgacgtcata accctgatta tcgagcactt caaccacacc
    75541 ttgcaggtct cgctccactt tacgacgcga aacatgggcc agctggatgt catttaataa
    75601 tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt cttcacgact
    75661 taacttcccc agcaggctat gatgggaaat attatcttcg tcaatgtatt ccgtcaggag
    75721 cggcaaaacc tcctgcatag gtacaatgcc gatggtcagg atcgccaacg acgcactcat
    75781 tgttcatcca ccttactttt ctttcgtcgt taattaccgg gcaagtgtga agcaccactg
    75841 ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacgtgc acggcataat
    75901 gtaaagattc caaatgattc
//
LOCUS       DAZGCS010000007        72916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGCS010000007 DAZGCS010000000
VERSION     DAZGCS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.007"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..145)
                     /gene="yojO"
                     /locus_tag="ACHK01_000496"
     CDS             complement(<1..145)
                     /gene="yojO"
                     /locus_tag="ACHK01_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO8095498.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLV"
     gene            254..748
                     /gene="napF"
                     /locus_tag="ACHK01_000497"
     CDS             254..748
                     /gene="napF"
                     /locus_tag="ACHK01_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO8095499.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDWQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            738..1001
                     /gene="napD"
                     /locus_tag="ACHK01_000498"
     CDS             738..1001
                     /gene="napD"
                     /locus_tag="ACHK01_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO8095500.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            998..3484
                     /gene="napA"
                     /locus_tag="ACHK01_000499"
     CDS             998..3484
                     /gene="napA"
                     /locus_tag="ACHK01_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO8095501.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3491..4186
                     /gene="napG"
                     /locus_tag="ACHK01_000500"
     CDS             3491..4186
                     /gene="napG"
                     /locus_tag="ACHK01_000500"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO8095502.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4173..5036
                     /gene="napH"
                     /locus_tag="ACHK01_000501"
     CDS             4173..5036
                     /gene="napH"
                     /locus_tag="ACHK01_000501"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO8095503.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5033..5482
                     /gene="napB"
                     /locus_tag="ACHK01_000502"
     CDS             5033..5482
                     /gene="napB"
                     /locus_tag="ACHK01_000502"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO8095504.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5492..6094
                     /gene="napC"
                     /locus_tag="ACHK01_000503"
     CDS             5492..6094
                     /gene="napC"
                     /locus_tag="ACHK01_000503"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO8095505.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6107..6730
                     /gene="ccmA"
                     /locus_tag="ACHK01_000504"
     CDS             6107..6730
                     /gene="ccmA"
                     /locus_tag="ACHK01_000504"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO8095506.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6727..7389
                     /gene="ccmB"
                     /locus_tag="ACHK01_000505"
     CDS             6727..7389
                     /gene="ccmB"
                     /locus_tag="ACHK01_000505"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO8095507.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7431..8168
                     /gene="ccmC"
                     /locus_tag="ACHK01_000506"
     CDS             7431..8168
                     /gene="ccmC"
                     /locus_tag="ACHK01_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO8095508.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8165..8374
                     /gene="ccmD"
                     /locus_tag="ACHK01_000507"
     CDS             8165..8374
                     /gene="ccmD"
                     /locus_tag="ACHK01_000507"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO8095509.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8371..8850
                     /gene="ccmE"
                     /locus_tag="ACHK01_000508"
     CDS             8371..8850
                     /gene="ccmE"
                     /locus_tag="ACHK01_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO8095510.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8847..10790
                     /gene="ccmF"
                     /locus_tag="ACHK01_000509"
     CDS             8847..10790
                     /gene="ccmF"
                     /locus_tag="ACHK01_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO8095511.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10787..11344
                     /gene="dsbE"
                     /locus_tag="ACHK01_000510"
     CDS             10787..11344
                     /gene="dsbE"
                     /locus_tag="ACHK01_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO8095512.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11341..12393
                     /gene="ccmH"
                     /locus_tag="ACHK01_000511"
     CDS             11341..12393
                     /gene="ccmH"
                     /locus_tag="ACHK01_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO8095513.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12428..13075)
                     /gene="narP"
                     /locus_tag="ACHK01_000512"
     CDS             complement(12428..13075)
                     /gene="narP"
                     /locus_tag="ACHK01_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO8095514.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13395..15986
                     /gene="yejO"
                     /locus_tag="ACHK01_000513"
                     /pseudo
     CDS             13395..15986
                     /gene="yejO"
                     /locus_tag="ACHK01_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16090..16166)
                     /locus_tag="ACHK01_000514"
     tRNA            complement(16090..16166)
                     /locus_tag="ACHK01_000514"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16130..16132),aa:Pro,seq:ggg)
     gene            complement(16241..18001)
                     /gene="yejM"
                     /locus_tag="ACHK01_000515"
     CDS             complement(16241..18001)
                     /gene="yejM"
                     /locus_tag="ACHK01_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO8095515.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(18021..18248)
                     /gene="yejL"
                     /locus_tag="ACHK01_000516"
     CDS             complement(18021..18248)
                     /gene="yejL"
                     /locus_tag="ACHK01_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO8095516.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18430..19437
                     /gene="yejK"
                     /locus_tag="ACHK01_000517"
     CDS             18430..19437
                     /gene="yejK"
                     /locus_tag="ACHK01_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO8095517.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19576..19860)
                     /gene="rplY"
                     /locus_tag="ACHK01_000518"
     CDS             complement(19576..19860)
                     /gene="rplY"
                     /locus_tag="ACHK01_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO8095518.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19985..21745)
                     /gene="radD"
                     /locus_tag="ACHK01_000519"
     CDS             complement(19985..21745)
                     /gene="radD"
                     /locus_tag="ACHK01_000519"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8095519.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21894..22589
                     /gene="rsuA"
                     /locus_tag="ACHK01_000520"
     CDS             21894..22589
                     /gene="rsuA"
                     /locus_tag="ACHK01_000520"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO8095520.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22617..23807
                     /gene="bcr"
                     /locus_tag="ACHK01_000521"
     CDS             22617..23807
                     /gene="bcr"
                     /locus_tag="ACHK01_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO8095521.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSFILFCLYASR
                     PKKR"
     gene            24140..24484
                     /gene="yejG"
                     /locus_tag="ACHK01_000522"
     CDS             24140..24484
                     /gene="yejG"
                     /locus_tag="ACHK01_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO8095522.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24488..26077)
                     /gene="yejF"
                     /locus_tag="ACHK01_000523"
     CDS             complement(24488..26077)
                     /gene="yejF"
                     /locus_tag="ACHK01_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO8095523.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26079..27104)
                     /gene="yejE"
                     /locus_tag="ACHK01_000524"
     CDS             complement(26079..27104)
                     /gene="yejE"
                     /locus_tag="ACHK01_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8095524.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27104..28198)
                     /gene="yejB"
                     /locus_tag="ACHK01_000525"
     CDS             complement(27104..28198)
                     /gene="yejB"
                     /locus_tag="ACHK01_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO8095525.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMIWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28199..30013)
                     /gene="yejA"
                     /locus_tag="ACHK01_000526"
     CDS             complement(28199..30013)
                     /gene="yejA"
                     /locus_tag="ACHK01_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO8095526.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(30095..31651)
                     /gene="pdeN"
                     /locus_tag="ACHK01_000527"
     CDS             complement(30095..31651)
                     /gene="pdeN"
                     /locus_tag="ACHK01_000527"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8095527.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31832..32398)
                     /gene="mepS"
                     /locus_tag="ACHK01_000528"
     CDS             complement(31832..32398)
                     /gene="mepS"
                     /locus_tag="ACHK01_000528"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO8095528.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRILSRS"
     gene            complement(32810..33523)
                     /gene="lpxT"
                     /locus_tag="ACHK01_000529"
     CDS             complement(32810..33523)
                     /gene="lpxT"
                     /locus_tag="ACHK01_000529"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO8095529.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VLLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(33562..34548)
                     /gene="yeiR"
                     /locus_tag="ACHK01_000530"
     CDS             complement(33562..34548)
                     /gene="yeiR"
                     /locus_tag="ACHK01_000530"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO8095530.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(34666..36132)
                     /gene="yeiQ"
                     /locus_tag="ACHK01_000531"
                     /pseudo
     CDS             complement(34666..36132)
                     /gene="yeiQ"
                     /locus_tag="ACHK01_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(36355..36927)
                     /gene="yeiP"
                     /locus_tag="ACHK01_000532"
     CDS             complement(36355..36927)
                     /gene="yeiP"
                     /locus_tag="ACHK01_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO8095531.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            37082..37336
                     /gene="yeiW"
                     /locus_tag="ACHK01_000533"
     CDS             37082..37336
                     /gene="yeiW"
                     /locus_tag="ACHK01_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8095532.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(37333..38511)
                     /gene="setB"
                     /locus_tag="ACHK01_000534"
     CDS             complement(37333..38511)
                     /gene="setB"
                     /locus_tag="ACHK01_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO8095533.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMGTAAGLEIPTMLIAGYFAK
                     RLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLMP
                     GQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV
                     "
     gene            38879..40009
                     /gene="fruB"
                     /locus_tag="ACHK01_000535"
     CDS             38879..40009
                     /gene="fruB"
                     /locus_tag="ACHK01_000535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO8095534.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSAPTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40009..40947
                     /gene="fruK"
                     /locus_tag="ACHK01_000536"
     CDS             40009..40947
                     /gene="fruK"
                     /locus_tag="ACHK01_000536"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO8095535.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            40964..42655
                     /gene="fruA"
                     /locus_tag="ACHK01_000537"
     CDS             40964..42655
                     /gene="fruA"
                     /locus_tag="ACHK01_000537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO8095536.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(42748..42894)
                     /locus_tag="ACHK01_000538"
     CDS             complement(42748..42894)
                     /locus_tag="ACHK01_000538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095537.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            43079..44020
                     /gene="psuK"
                     /locus_tag="ACHK01_000539"
     CDS             43079..44020
                     /gene="psuK"
                     /locus_tag="ACHK01_000539"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO8095538.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            44008..44946
                     /gene="psuG"
                     /locus_tag="ACHK01_000540"
     CDS             44008..44946
                     /gene="psuG"
                     /locus_tag="ACHK01_000540"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO8095539.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            45040..46290
                     /gene="psuT"
                     /locus_tag="ACHK01_000541"
     CDS             45040..46290
                     /gene="psuT"
                     /locus_tag="ACHK01_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8095540.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFSFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46361..47059)
                     /gene="yeiL"
                     /locus_tag="ACHK01_000542"
     CDS             complement(46361..47059)
                     /gene="yeiL"
                     /locus_tag="ACHK01_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO8095541.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            47189..48130
                     /gene="rihB"
                     /locus_tag="ACHK01_000543"
     CDS             47189..48130
                     /gene="rihB"
                     /locus_tag="ACHK01_000543"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO8095542.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            48230..49480
                     /gene="nupX"
                     /locus_tag="ACHK01_000544"
     CDS             48230..49480
                     /gene="nupX"
                     /locus_tag="ACHK01_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8095543.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49536..50624)
                     /locus_tag="ACHK01_000545"
     CDS             complement(49536..50624)
                     /locus_tag="ACHK01_000545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8095544.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(50628..51485)
                     /gene="nfo"
                     /locus_tag="ACHK01_000546"
     CDS             complement(50628..51485)
                     /gene="nfo"
                     /locus_tag="ACHK01_000546"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO8095545.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(51559..52608)
                     /gene="yeiH"
                     /locus_tag="ACHK01_000547"
     CDS             complement(51559..52608)
                     /gene="yeiH"
                     /locus_tag="ACHK01_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO8095546.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            52707..53588
                     /gene="yieE"
                     /locus_tag="ACHK01_000548"
     CDS             52707..53588
                     /gene="yieE"
                     /locus_tag="ACHK01_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO8095547.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            53793..55262
                     /gene="lysP"
                     /locus_tag="ACHK01_000549"
     CDS             53793..55262
                     /gene="lysP"
                     /locus_tag="ACHK01_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO8095548.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPH
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            55555..57546
                     /gene="cirA"
                     /locus_tag="ACHK01_000550"
     CDS             55555..57546
                     /gene="cirA"
                     /locus_tag="ACHK01_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO8095549.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(57578..58414)
                     /gene="yeiG"
                     /locus_tag="ACHK01_000551"
     CDS             complement(57578..58414)
                     /gene="yeiG"
                     /locus_tag="ACHK01_000551"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8095550.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            58672..59340
                     /gene="folE"
                     /locus_tag="ACHK01_000552"
     CDS             58672..59340
                     /gene="folE"
                     /locus_tag="ACHK01_000552"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO8095551.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            59357..60514
                     /gene="yeiB"
                     /locus_tag="ACHK01_000553"
     CDS             59357..60514
                     /gene="yeiB"
                     /locus_tag="ACHK01_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO8095552.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            60656..61696
                     /gene="galS"
                     /locus_tag="ACHK01_000554"
     CDS             60656..61696
                     /gene="galS"
                     /locus_tag="ACHK01_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO8095553.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDSRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            61976..62974
                     /gene="mglB"
                     /locus_tag="ACHK01_000555"
     CDS             61976..62974
                     /gene="mglB"
                     /locus_tag="ACHK01_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO8095554.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            63035..64555
                     /gene="mglA"
                     /locus_tag="ACHK01_000556"
     CDS             63035..64555
                     /gene="mglA"
                     /locus_tag="ACHK01_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO8095555.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            64571..65581
                     /gene="mglC"
                     /locus_tag="ACHK01_000557"
     CDS             64571..65581
                     /gene="mglC"
                     /locus_tag="ACHK01_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO8095556.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(65652..66887)
                     /gene="preA"
                     /locus_tag="ACHK01_000558"
     CDS             complement(65652..66887)
                     /gene="preA"
                     /locus_tag="ACHK01_000558"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO8095557.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(66881..68119)
                     /gene="preT"
                     /locus_tag="ACHK01_000559"
     CDS             complement(66881..68119)
                     /gene="preT"
                     /locus_tag="ACHK01_000559"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO8095558.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLLLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(68438..68677)
                     /gene="yeiS"
                     /locus_tag="ACHK01_000560"
     CDS             complement(68438..68677)
                     /gene="yeiS"
                     /locus_tag="ACHK01_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO8095559.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(68680..69399)
                     /gene="sanA"
                     /locus_tag="ACHK01_000561"
     CDS             complement(68680..69399)
                     /gene="sanA"
                     /locus_tag="ACHK01_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO8095560.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(69549..70433)
                     /gene="cdd"
                     /locus_tag="ACHK01_000562"
     CDS             complement(69549..70433)
                     /gene="cdd"
                     /locus_tag="ACHK01_000562"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO8095561.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEEALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(70563..71258)
                     /gene="yohK"
                     /locus_tag="ACHK01_000563"
     CDS             complement(70563..71258)
                     /gene="yohK"
                     /locus_tag="ACHK01_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO8095562.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(71255..71653)
                     /gene="yohJ"
                     /locus_tag="ACHK01_000564"
     CDS             complement(71255..71653)
                     /gene="yohJ"
                     /locus_tag="ACHK01_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO8095563.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            71892..72842
                     /gene="dusC"
                     /locus_tag="ACHK01_000565"
     CDS             71892..72842
                     /gene="dusC"
                     /locus_tag="ACHK01_000565"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO8095564.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT        17631 a        17925 c        19475 g        17885 t
ORIGIN      
        1 aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatattta taaccatttg
       61 aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat cctgctacag
      121 gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta gcggctataa
      181 aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag cgcgttaatg
      241 atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg gtcgctggcg
      301 caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt ttctgaccca
      361 ttgcacccgc tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc aacgcggcgc
      421 gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt acgcctgcgc
      481 gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg attggcagtt
      541 caccattggg gacgcgtgcc tggcgtatca gtctgttgaa tgccgccgct gtcaggatag
      601 ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc agccgcaact
      661 taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg tatcagccat
      721 aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc gtgcaggcca
      781 aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc tgtgaagttg
      841 ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa gacagcgaaa
      901 cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg gtgtcgctgg
      961 tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc gtcgtagctt
     1021 tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg tgccgggcgt
     1081 tgcccgcgcc gttgttggtc agcaggaagc catcaaatgg gataaagcgc cgtgccgttt
     1141 ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg tggcctgtca
     1201 gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct atttcctgcc
     1261 caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga aaaacggtaa
     1321 atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg atgtgatgga
     1381 agagaaattc aaaaccgccc tgaaagaaaa aggaccggaa tcgatcggta tgttcggttc
     1441 tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgttcaaag cgggcttccg
     1501 ttcgaacaac atcgacccga acgcacgtca ctgtatggcg tcggcagtag ttggctttat
     1561 gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc aggctgacgc
     1621 gtttgtgctg tggggcgcta acatggcgga gatgcacccg atcctctggt cacgcatcac
     1681 taaccgtcgt ctctctaacc agaacgtcac cgtggcggtg ctttctacct accagcatcg
     1741 tagcttcgag ctggcggata acggcatcat ctttacgccg caatctgacc tggtgatcct
     1801 gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact tcttcagcaa
     1861 gcacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc cgacccatcc
     1921 gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga gctttgaaga
     1981 ttacaaagcc ttcgttgccg agtatacgct ggaaaaaact gccgaaatga ccggcgtgcc
     2041 gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga aagtcatctc
     2101 ctactggacg atgggcttca accagcatac tcgtggcgtg tgggccaaca acctggtcta
     2161 caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt tctccctgac
     2221 cgggcagcct tccgcgtgtg gtactgcgcg tgaagtgggc acctttgctc accgtctgcc
     2281 tgcggacatg gtggtgacta acgagaaaca ccgtgatatt tgcgagaaga agtggaatat
     2341 cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag accgtgcact
     2401 gaaagacggc aagctgaatg tttactggac catgtgtacc aacaacatgc aggccgggcc
     2461 gaacattaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca tcatcgtctc
     2521 cgatccgtat ccgacagtca gtgcgctggc agccgacttg atcctgccga ccgcaatgtg
     2581 ggtagagaaa gagggcgctt acggtaacgc cgaacgccgt actcagttct ggcgtcagca
     2641 ggtacaggcg ccaggcgaag cgaaatcgga tctctggcag ttagtccagt tctcccgccg
     2701 cttcaaaact gaagaagtat ggccggaaga gctgctggcg aagaaaccgg aactgcgtgg
     2761 caaaacgctg tacgaagttc tgtatgccac ccccgaagtg agcaaattcc cggtatccga
     2821 actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc tgcaaaaagg
     2881 gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg cgccgttcga
     2941 tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag aaacacagtg
     3001 gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata agttctacgg
     3061 taaaccggat ggcaaagcgg tgatcttcgc actgccgttc gaaccggcgg cggaagcacc
     3121 ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact ggcacaccgg
     3181 cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg tcttgtttat
     3241 tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga aagtggtttc
     3301 tcgccgtggt gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc cgccacaggg
     3361 cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactga cgctggatgc
     3421 gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac tggagaaggt
     3481 gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct ttctgcgcga
     3541 tgtcgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt tacaacagca
     3601 aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg agaacgcctt
     3661 tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg acaccttaaa
     3721 actggcgacg ctggcctctg gtctgtcggc gggcacgcca tattttgtcg cacgggatat
     3781 tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccaagcg gtgcgctgga
     3841 tcgtgagatt gaatcgatcg acgacgcgcg gatggggctg gcggtactgg tggaccagga
     3901 aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat gcccgaaaat
     3961 tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc acgcccgctt
     4021 tctgccgacg gttcacagcg acgcctgtac cggttgcggt aagtgcgaaa aagtatgcgt
     4081 gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg agttaggtca
     4141 ccattaccgc ttcggttggc tggaggggaa caatggcaaa tcgtaaacgt gacgccgggc
     4201 gcgaggcgct ggagaaaaaa ggctggtggc gcagtcaccg ttggctggtg ttacgtcgcc
     4261 tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc atggtttggt gtgtggatct
     4321 tgcacggcaa ctacagcagt agcctgttat tcgacaccgt tccgctgacc gatccgctga
     4381 tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg accggtgcgg
     4441 tgattatcac cgtgctctac gccctggcgg gaaagcgatt attttgcagc tgggtctgcc
     4501 cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac ctcaatcagt
     4561 ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg ctgggatcgg
     4621 ccttaactgg cacgctcatc tgggaatgga tcaaccccgt atctctgatg gggcgcagcc
     4681 tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta tttgatctac
     4741 tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg tatggcgtgc
     4801 tgggtagcaa aggtgtgatt accgttgcgg ccaccgatcg gcagaaatgt aaccgctgta
     4861 tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg ctggatgaac
     4921 aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc gtggatgtct
     4981 gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa tcatgaaaag
     5041 ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg tggtaagcgg
     5101 cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat ccgggacaca
     5161 ggaaggggcc attcggatgc caaaagagca ggaacggatg ccgctgaact atgtgaatca
     5221 gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata ccaaccgctg
     5281 cttgcagtgc cacggtgtcg aaagttatcg caccactggc gcgccgcgta tcagtcctac
     5341 tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc gttatttctg
     5401 tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata cctttacccc
     5461 atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta agcctggtct
     5521 gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg ggacgctgct
     5581 gttgatcggt tttgttggcg gcatcgtctt ctggggtggc tttaacaccg ggatggaaaa
     5641 agccaatacc gaagagttct gcattagctg ccacgaaatg cgcaacacgg tgtatcagga
     5701 atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct gtccggattg
     5761 tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa gtaaagagct
     5821 gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc atcgtctgac
     5881 gatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt gccgtaactg
     5941 tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga agatgcatga
     6001 ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag cgcacaagct
     6061 gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg gtatgcttga
     6121 agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct tgtcatttac
     6181 gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga agacaacgct
     6241 tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct ggcaagggca
     6301 gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag gccatcagcc
     6361 ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc gcgatggcga
     6421 taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg aagatattcc
     6481 tgtaaatcag ctctcggccg ggcaacaacg ccgcgtcgct ttagcgcgtc tgtggctgac
     6541 ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta acggcgtcga
     6601 tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga ttctgactac
     6661 ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga cgcaaacgag
     6721 ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt tcgccatagc
     6781 gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt tccgctcagt
     6841 atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg ggttgctgcg
     6901 ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca ggacggcagt
     6961 cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc gaaggtgatg
     7021 gcgcactgga tggtaaccgg tctgccgtta ctcattcttt cgccactggt agcaatgcta
     7081 ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct gggaacgcct
     7141 acgcttggct ttctcggtgc accgggcgtg gcgctgacag tgggacttaa gcgcggtggt
     7201 gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt cgccaccgcc
     7261 gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat tttaggcgcg
     7321 ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt acgaatcagc
     7381 attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact atgtggaaaa
     7441 cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg tttataccgt
     7501 ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga ttcggctttg
     7561 ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat gtgcctgcgg
     7621 cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt ggccttgtct
     7681 ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt gccgtgttta
     7741 cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc acctggtggg
     7801 tatgggatgc acgtctgact tctgaactgg tgctgctttt tttgtatgtg ggtgtgattg
     7861 ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt atcctggtgc
     7921 tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg aacaccctgc
     7981 atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg ccgctgcgct
     8041 ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg atgcgtaatt
     8101 tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg aaaagaggcc
     8161 gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg gttacgcctt
     8221 ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg tcgtgcactc
     8281 ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc gtgaggcgcg
     8341 tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa ccgcttgtgg
     8401 attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct atatgcgctg
     8461 cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa gcgtgaaact
     8521 cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat gccgggtagt
     8581 gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc tgaaggctca
     8641 gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca gggcgttgtg
     8701 gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct ggcgaaacac
     8761 gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca ccgtcgcccg
     8821 gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg ctgctgtgcc
     8881 tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg gcgcgcggag
     8941 atgcgcgcat gatggcgtct tcccgcttgt ttgcctggct gctgtttatg tctgtggctg
     9001 gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc acctatgttg
     9061 ccagcaactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc tggggcgcgc
     9121 atgaaggctc gctcctgctg tgggtgctgc tgatgagcgg ctggaccttt gcggtggcga
     9181 tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata atggggatgg
     9241 tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca cgcacgctgc
     9301 cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggatccg gggctgatct
     9361 tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt gcttttgcca
     9421 ttgcttcttt gttgtgcggg cgtctggaca gcacttatgc gcgttttact cgtccgtgga
     9481 cgctggcggc gtggatcttc ttgacgctcg gcattgtgct cggttccgca tgggcctatt
     9541 acgaactcgg ctggggcggc tggtggttct gggatccggt agaaaacgcc tcgtttatgc
     9601 cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa cgcgccagct
     9661 tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg ctggggactt
     9721 tcctggtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat ccggcacgcg
     9781 gtatgtttat cctcgccttt atggtactgg tgattggtgg ttcgctgctg ctgtttgccg
     9841 cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg gaatctctgc
     9901 tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtattactg gggacgctgc
     9961 tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa ccgttcttca
    10021 acaccatgtt tacctggctg atggtgccgt ttgcgctgct gcttggtgtc ggtcctctgg
    10081 tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc gccttcatct
    10141 caacgctggt gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt gtggcgatga
    10201 cggtgctcgg cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg gaagctgcgc
    10261 tgcgtatttc acgtggcacg aaaaccacct tcagttattg gggaatggtg gcggctcacc
    10321 tggggctggc agtgacaatt gttggtattg cctttagcca gaactatagc gttgagcgtg
    10381 atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc accttccgtg
    10441 atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc ggcgtaacgc
    10501 gcgatggcaa gccggaaacg gtgctgtatg cggaaaaacg ttattacaac actgccgggt
    10561 cgatgatgac cgaagcggca attgacggcg gcatcacgcg tgacctgtac gccgcgctcg
    10621 gtgaagagct ggaaaacggc gcgtgggccg tgcgtcttta ctacaaacca tttgttcgct
    10681 ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg tttgatcctc
    10741 gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggccgtatga agcgcaaagt
    10801 attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt ggcagctggc
    10861 gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg gcaagcctgt
    10921 gccgaagttt cgtctcgaat cactggacaa tccggggcag ttttatcagg cggatgtgct
    10981 gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga cctgccgtgc
    11041 ggaacatcaa tatctgaatc agctttcagc gcagggcatt cgcgtggtcg gcatgaacta
    11101 taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc cttacgcgct
    11161 aagcctgttt gatggcgacg gcatgttagg gctggatctc ggtgtctatg gcgcgccaga
    11221 aaccttcctt attgacggca acggtatcat tcgctatcgc catgcgggcg atctcaatcc
    11281 tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg aggccgcaca
    11341 atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcact ggcgaccatc
    11401 gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct cactgaagaa
    11461 ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat gattgccacc
    11521 gacctgcgtc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa agagattgtc
    11581 gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt aacgccgctg
    11641 accgtgctgc tgtgggtgct tccggtagtg gctattggca ttggcggttg ggtcatatac
    11701 gcccgctcgc ggcgtcgggt acgcgtagtg ccggaagcgt ttcctgaaca aagcgtgcag
    11761 gaaggtaagc gtgccggata tattgtttat ctgccgggta ttgtggtggc gttaattgtg
    11821 gctggcgtca gctactacca gactggcaat tatcagcagg tgaaaatctg gcagcaggcc
    11881 acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagccga tccgctcaac
    11941 gaagaagaga tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa aaatccggga
    12001 gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa cgctagtatc
    12061 gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagcga tgccgcactg
    12121 ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct cggtggtgaa
    12181 ctgctgcgcc agttggtgag aagcgaccac agcaatatcc gtgtgttaag catgtatgcg
    12241 tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga gatgatgttg
    12301 aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat cgcgcaggcg
    12361 atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg atgccatcgg
    12421 gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggctgcc acgcgtgagc
    12481 ggacattgag tttgcgcagc agattgcgaa tatgtacttt tactgtctgc tctgaaatat
    12541 tcaacaccga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc agaacatcca
    12601 gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt tcacgttcac
    12661 gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctcca gcacgaattg
    12721 cttccagcaa tacttccggg tcgctgtctt tcaacagata accgtctgcg cctgcgtcta
    12781 tcagtgcaaa gacatcgctg gaggcatctg atacggtcag gataataatt tgcgcggtaa
    12841 cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc atattgagat
    12901 ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc gcgccgtcgc
    12961 ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga acaccgcgtc
    13021 gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca ggcatagtag
    13081 tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac gctaccaggc
    13141 gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac cttgtcgttc
    13201 gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca tcaagaaaaa
    13261 caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta aaaactgact
    13321 ccgggtatgg agctatgggt attttccgta cccaatgctt ttaacagcaa ttaatttcat
    13381 aggatgaaag ctcaattcat caatctggtt ctgtttctct ttgtcgttcc gcaatatctg
    13441 ttctgatggc tacagcgtta tattcaccca tagcattggc atcaacagtt gagtatggtg
    13501 agacagttga tggtgttgtc ctggaaaaag atatccagct ggtttatggg accgccaata
    13561 atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta agtagtaata
    13621 ctgaaattaa aggagggtat cagtacattg aaatgaatgg taccgcagaa tactcagtat
    13681 taaatgacgg ttatcaaatt gttcaaatgg gtggcgcggc aaaccagact accctcaata
    13741 atggtgtgct acaggtttat ggcgcagcga atgatcccac gattaaaggc gggcgcttaa
    13801 tcgttgaaaa agatgggatt accgtccttg ccgctatcga aaagggagga ttactggagg
    13861 ttaaagaggg gggattagcg attgcggtag atcagaaagc aggcggtgct attaaagcaa
    13921 gcacgcgggc catggaggta ttcggaacaa accgtctcgg tcagttcgat atcaagaatg
    13981 gtattgctaa taatatgttg ttggaaaacg gcggaagttt gcgagttgaa gaaaatgact
    14041 tcgcttataa taccactgta gatagtggcg gcttactgga ggttatggat ggcgggactg
    14101 taactggcgt tgataaaaaa gcaggcggaa aattaattgt ctcaacgaat gcgctggaag
    14161 tgagtggtcc aaacagtaaa ggccaattta gtataaaaga tggtgtgtca aaaaattatg
    14221 aactggatga tggttccggg ctcattgtta tggaggacac gcaggccatt gatactatcc
    14281 ttgatgagca tgccactatg caatcgctgg gaaaggatac tggtacgaaa gtgcaggcaa
    14341 atgcggtata tgatctcggt cgatcagatc agaatggaag tatcacgtat tcctcaaaag
    14401 ccatctctga aaatatggtt atcaacaatg gccgcgctaa cgtctgggct ggcacaatgg
    14461 ttaacgtttc agtcagaggg aatgatggca ttcttgaggt catgaagccg caaataaatt
    14521 atgcacccgc aatgttggtg ggtaaggtag tggtttctga gggcgcttct tttagaacgc
    14581 atggtgccgt ggataccagc aaagcggacg tttcgctcga aaatagcgta tggaccatca
    14641 ttgccgatat cactacgacg aaccaaaaca ccctcctcaa cttagccaac cttgcgatgt
    14701 ctgacgcaaa tgtgattatg atggatgagc cagtgactcg ttcatcagtg acggcaagtg
    14761 cggaaaattt cattacgttg accaccaata ccctgtcggg aaacggcaat ttttatatgc
    14821 gtaccgatat ggctaatcat cagagcgatc agctcaacgt caccggtcag gcaacaggtg
    14881 atttcaaaat attcgtgacg gacaccggtg ccagcccggc agcaggagat agccttacac
    14941 tggtaacaac gggcggcggt gatgctgcat ttacgttggg caatgccgga ggcgttgttg
    15001 atatcggtac gtatgaatat accttgctgg ataatggcaa ccatagctgg agtttggcag
    15061 agaatcgcgc gcaaattacc ccttcaacca ctgatgtgct gaatatggcg gcagcacaac
    15121 cgctggtatt tgatgcagaa ctggacaccg tgcgtgagcg tcttggtagc gtaaaaggcg
    15181 ttaattacga tacggcgatg tggagttcgg caattaacac ccgcaacaac gtgaccactg
    15241 atgcgggcgc tggctttgag caaacattga ccgggctgac gctcggtatc gatagccgtt
    15301 tctcccgtga agaaaccagc acaattcgcg gcttgttctt tggttactct cattctgata
    15361 ttggttttga tcgcggcggc aaaggcaatg tcgatagcta taccctgggg gcttatgccg
    15421 gttgggagca tcagaacggt gcctatgttg atggagtggt gaaagttgac cgttttgcca
    15481 acaccatcca ttgcaagatg agtaatgggg caacagcgtt tggcgattac aatagtaacg
    15541 gcgcgggtgc tcatgtcgag agcgggttcc gttgggttga cggattgtgg agtgttagac
    15601 cctatctggc ctttaccggc tttaccacag atggtcagga ctacacgtta tcaaacggca
    15661 tgcgcgcgga tgtgggaaat acccggatat tacgcgctga agcgggaacg gcggtaagct
    15721 atcacatgga cctgcaaaac ggtacgacgc tggaaccctg actgaaagcc gccgtgcgtc
    15781 aggaatacgc cgattctaac caggtgaaag ttaatgacga tggcaaattt aataatgatg
    15841 tggctggaac ccgtggcgtt tatcaggctg ggataaggtc atcgtttacc ccgacgttaa
    15901 gcggtcattt gtcagtcagc tatggcaatg gcgcaggggt agaatcgccg tggaatactc
    15961 aggcgggtgt ggtctggacg ttctgataac aaaaaataaa caggctgtga tgtgtcacgg
    16021 tctgtttatc gaattaattg cagatataaa aaaaaccaac cgtaaaggtt ggttttttct
    16081 tgggattttt ggtcggcacg agaggatttg aacctccgac ccccgacacc ccatgacggt
    16141 gcgctaccag gctgcgctac gtgccgactc gtggctgcta atactaccgt tttccacacc
    16201 gattgcaagt aagatatttt gctaactgat ttataattaa tcagttagcg ataaaacgct
    16261 tctcgtctgt cagcacttgc aataacaaac ttaactgtgg tttttcatct ttcactcttt
    16321 caccacgtaa gttgtaagtg cggtatttac cgttattgtt cagcaccagc gtctttttcg
    16381 gggtggtaat tgccagcgta tcgttatccg ctgcagtaac ccagtaatga cggcgttgag
    16441 ggttgaacaa atcctgacct tgcgaatatt cgctggcagg tgtgctgaca tgtagcaggc
    16501 gttgcatcag cgtcgtcatc agatcggtat gatcagtcag agcattaata cgctgcgccg
    16561 gcgtgcctgg ccagtgaatc actaatggca cctgcagatg accgtgggac cagtcaaagg
    16621 tttcttcgtc ttcgctcagc ggaatacccc gaccggcagt gataatcacc accgtattgt
    16681 ccagtttgcc agaatcacgc agtgcattga gcacgcggtt gatctggtca tcgacattgc
    16741 ctgccgcccg gctatatttc cgtgcaaatg cctgctgatt gctgtcgtca atgttagtgc
    16801 cattgaaaga gacccacgag aaccagcggt tatcttcttg tgcgtagcgg cccagccagt
    16861 tgatccactg cgtggcggtc tgctcgtcgg attgggtgcg tacgctcggc atcgagaaat
    16921 ctgacaacaa tgcctggcga tacagcgggc tggtaaagcc atctgatgag aataacccca
    16981 gctgatagcc ttgctgatta agcgcagtaa ttaatgccgc aggcgtacgg gtcgacagaa
    17041 tgccgtccat atagctcggc gagatgccat agaacaggcc aaagatgccg ttgtctgtag
    17101 tgttgccgga gctcatatgg cgcgtgaacg aaatattttg ctcagcaaaa cctgccagcg
    17161 caggcatctg cttctcgaag cgtgagtagt tcaggccatc gacagtaatc aacagcacat
    17221 tctgcccggt gcccatatcg cgatagcgca gttcgcttaa cggatactga acggaaacgg
    17281 cgtctggatt accttgctca ataagacggc gttgatactc ctgcgcatca agcagaccat
    17341 gcttctcaag aaaacgtcgc gccgtcatcg ggtacgaaag cggcaggtta gcgcgctgca
    17401 tggtgatcgg gcgatagaag ttggcatcgg cccagatata caccacatgc gaggcgataa
    17461 aggcgataaa taagaatgcg gccagcgggc gcgcgaagcg tcgacgacgc gtcaggctgc
    17521 gcagcttttg ccagctccac gtcgcaaaca ccagttcaag caataaaata accggcacgc
    17581 tgatgaacat cagctgccag tcgcgcgcca tctcattttc gtctgggttg ataaccagtt
    17641 gccagacgat gggattaaga tggagatgga aacgagtaaa gacttcgcta tcgatcagta
    17701 atagcgtcat tcccgccgtt gccagaatga cggacaaaaa cctcatcagc ctctgggagc
    17761 cgacgataaa ggtcagcggg aagaggatca gcaagtaggt ggcgaacacc aggaagctga
    17821 aatggccgat aatgcttacg taggaataaa tgcgaccagc aagcgttgtc ggccagtcgg
    17881 cgataaacag gtaacggctg ccaatgacga gcgaaagcag aatattgaac agtgcaaacc
    17941 agtgccccca actgaccatc tgggagactt tttcacggta gcgctgacga tgagttacca
    18001 taacctgtta tctgtttccc ttagtgcgct ttgtcttcgt tgatagagga ctgtaaggcg
    18061 cgggcaaaag agttggcaat tgcctggcgt tgggccgggg caatgctggt gttgataagg
    18121 ttagtgacca tgttgcctaa caccatcagg gaaaggtcgg tcggagcctt atgtttttcc
    18181 agtacgttga gcagctctgc gagcaattgt tcaacctgtt catcactgta gcgggaaatt
    18241 tgtggcataa atcgaaatca gttagttgat gaaagggcaa catattaccg tagcaacggt
    18301 tttttttctg cttttttatg cgttaaatca ttctcgttac gctgccttct gtaaaatgac
    18361 cgccatcttt tattgcgtcg cttaaccggc ggtggttgaa taccgcccgg tcttaaagga
    18421 gagtttatca tgagtctgga tatcaaccag attgctctgc accagcttat caagcgtgat
    18481 gagcagaacc ttgagctggt gttgcgcgat tcattgctgg aaccgacaga aaccgtcgtt
    18541 gagatggtgg cagaactgca tcgggtctat agcgccaaaa ataaagctta cggcttgttt
    18601 agcgaagaga gtgaactggc gcagacgctg cgcttacagc gtcagggcga agaggacttt
    18661 ctggcattta gccgcgcggc aaccgggcgc ctgcgtgacg aattagcgaa atatcctttt
    18721 gctgacggtg gattcgtgct gttttgccac taccgttatc tggcggtgga gtatctgctg
    18781 gtggcagttc tgagcaacct gagcagtatg cgcgttaacg aaaatctcga catcaatcca
    18841 acccattatc ttgatatcaa tcatgcggat attgttgcgc gtatcgattt aaccgaatgg
    18901 gaaaccaatc cagagtccac ccgctatctc actttcctga aagggcgagt agggcgcaaa
    18961 gtggcggatt tctttatgga tttcctcggg gccagcgaag ggttaaacgc gaaagcgcaa
    19021 aaccgtgggc tgttgcaagc ggttgatgat ttcactgccg aagcacaact ggataaagca
    19081 gagcgacaga acgtgcgcca gcaggtttat agctactgta atgagcagct gcaggcaggc
    19141 gaagagatcg aactggagtc gctgtcgaaa gagctggcag gcgtcagcga agtgagtttc
    19201 acggagtttg cggctgaaaa aggctacgaa ctggaagaga gcttcccggc agaccgtagc
    19261 acactgcgtc agttgacgaa gtttgccggt agcggcggtg gcttgactat taactttgat
    19321 gcgatgctgt taggtgaacg catcttctgg gatccggcaa ccgataccct gaccattaaa
    19381 ggcacgccgc cgaatttgcg tgaccaattg cagcgccgga cgtctggcgg caattaatcg
    19441 gttttgtgtt ctgaatgcca gatgcggcgt gaacgcctta tctggcctgc aaaaaatagc
    19501 aataccgtaa tcaggcataa tctgcagata caaaaaaccc cgccggagcg gggtttttta
    19561 caacttattc agcaattaag cgcgaacgaa gtcgatgtgc tgcagcttcg gtttgtacgg
    19621 gtgacgctgt acgtcctgag ctttaacttt gatttcttta ccgtcaacaa cgatggtcag
    19681 aacttcgctg tagaattcag ctttagcttg catgttcatg actttgtcgt gatccagctc
    19741 gatagccagc ggcgcttctt tgccaccgta gatgattgcc gggaacttgt tagcggcacg
    19801 caggcggcgg ctcgcaccct taccctgctc tttacgtact tctgcgttga tagtaaacat
    19861 ttctttctct cttaaattaa tcctgctaca ggcgacccag caacaggtaa gtgatctgct
    19921 ttgcgtatgc aaaagcgggc gagattctat actcaatcgc tttatacatc aatcaaaact
    19981 accattaacc gcgtaattcg tgcgcccgac gaaaacgacc ttcgtaatcg aacacttttt
    20041 cacgcacttg ccagtactga cctttcatgc gggcaacgac aaaatccggg tgtcgcaata
    20101 aggcttgctg ggcgaggata tcggcggcgg tgatccagcg cagagggatg cccggtgtgc
    20161 gcgtatgcgg gcggataaaa agctgctcga aggcagttcg ctgggcgggc gtttgcagac
    20221 ggaaacgctc actcacatcc gcgccgtctt catcgtaata ggtgattttc aaccattcgc
    20281 ctttctcgtc atgcccatgc tgtaaagaca tgccgctaca gcgtaatacc agcgcgtctt
    20341 tcagtcgtag cgccgctttt aacatatcgt ctggatcaac cagtacggtg tcacattcgc
    20401 ggcagcggcg agcggcaata tcgttttccg cgttacattg cgggcaattt ttaaaacgga
    20461 aacggaagtc acattgttcg cgatgaccgt cgtcatcttc aaaccatccc tgacagcgac
    20521 gaccaaagtg ttcaatcaat gtcccgtcgg cggtcgtttt cccccaaaag gtgttggcaa
    20581 aaccgcaggc agggcagaaa acctgtaccg gaacgttgtc acttttgcct tttggtgtac
    20641 caacttccgg cgcgtagaga tcgtgaggat tacccgcata atcaagaatt aagcaatcag
    20701 tcttgcccgg agcgagacgc agaccgcgcc cgacaatttg ttggtaaaga ctcactgatt
    20761 cggtagggcg cagaatggcg ataagatcga cgtgcggggc gtcaaatccg gtggtcagta
    20821 ctgcgacgtt gaccagatag cgaaaacgct gggctttaaa atcttcaatt aacacatcgc
    20881 gctcagcgcc gggggtgtcg ccagtaatca gtgcagcatc ttcggcaggc agtaatccca
    20941 caatctcttt tgcgtgttca acggtggcgg cgaaaatcat tacccctttg cgcttttcgg
    21001 cgaactccat gatctggctg atgatatgtg gcgtaatgcg ttgctgtttt ttcagctcac
    21061 ggttgagatc agcttcgctg aacagcccgt tactctgtgc ctgcaagcgg ctgaaatcgt
    21121 attgcactac tggcatatcc agtcgttctg gcggcgtcag atagccgtgt ttaatcatat
    21181 aacgcagcgg cagctcataa atgcagtcac ggaaaagggc tttctcatcg ccgcgtacca
    21241 tgccgtgata atggaactgg tagatccagc ctttgcccaa tcgaaaaggc gtggcagtca
    21301 gccccagcag gcgtaagtgg ggattcactt tagtcaggtg agtgaggatt tgctgatact
    21361 ggctctcttc atcgtcacca atacggtggc attcatcgac aatcaacagc gaaaattcac
    21421 cctgaaaggc atcaagatta cgagcgaccg actgcacgct gccaaatacc actttaccgt
    21481 ggctctcttt gcgctttaac ccagcggcaa aaatatcggc ttccagcccc agcgcctgat
    21541 actttgcatg gttttgcgcc accagttctt tgacgtgtgc cagcaccaga acacgaccac
    21601 gcgccagccg tgccagttcg gcaatcacca ggcttttacc tgcgccggtg ggcagcacga
    21661 taacggcagg ggttttatga cgacgaaaat ggttgagcgt ggcatccacg gcttcttgct
    21721 gatatgggcg gagtgtaaaa atcatggtct cgcgtaggta gattattctg ctaatagtat
    21781 gccatggatc tttttccctt gagggatgtt attagaccgc tatactgggc agacaacagt
    21841 tcagattttc gggtcaatgc ccgacctccg tttttacagg caaaatcaca cacatgcgac
    21901 ttgataaatt tatcgcacag caactcggcg ttagccgtgc tattgccggg cgtgaaatcc
    21961 gcggcaatcg tgtcaccgtc gatggcgaaa ttgttcgtaa tgcagcgttc aaactgcttc
    22021 ctgaacatga tgtcgcttac gatggcaacc cgctggcgca gcaacatggt ccacgttact
    22081 tcatgctcaa taagcctcag ggctatgttt gctccacgga cgaccctgat cacccaacgg
    22141 tgctctattt tctggatgaa ccggtagcct ggaaactgca cgccgcagga cgtctggata
    22201 tcgataccac cggtctggtg ctgatgactg atgatggtca gtggtcgcac cgcattactt
    22261 ctccgcgcca tcattgcgag aagacctatc tggtgacact ggaatcacct gtagctgacg
    22321 atacggcaga gcaatttgct aaaggcgtgc agctgcataa cgaaaaagat ctcactaagc
    22381 ctgcggtgct ggaagtgatt accccaacgc aggttcgtct gaccatcagc gaagggcgtt
    22441 atcatcaggt gaaacgcatg ttcgccgccg tgggtaacca cgtggttgag ctgcatcgtg
    22501 aacgtattgg cggtattacg ctggatgctg atttagcccc cggtgaatat cgtccgttaa
    22561 ctgaagaaga aattgccagc gtcgtctaac ttttccatga ctttcaggag cccgttgtga
    22621 ccacccgaca gcattcgtcg tttgctattg tttttatcct tggcctgctg gccatgttga
    22681 tgccgctgtc gattgatatg tatctgcccg cgctaccggt aatttcagcg cagtttggcg
    22741 tacctgcggg cagtacgcag atgaccctca gtacttatat tctgggcttt gcgttggggc
    22801 agttaatcta cgggccgatg gcagacagct tcgggcgtaa gccggtggtg ctcggcggta
    22861 cgctggtgtt tgccgccgcc gcggtggcgt gtgcgttggc aaacaccatc gatcagctga
    22921 ttgtgatgcg tttcttccac gggctggcgg cggctgcggc cagcgtggtt atcaacgctt
    22981 tgatgcgcga tatttacccg aaagaagagt tctcgcggat gatgtcgttt gtcatgctgg
    23041 tgaccactat tgcaccgttg atggcaccga ttgtcggcgg ttgggtgctg gtgtggctga
    23101 gctggcatta catcttctgg atcctggcat tagcggcgat tctggcttcg gcaatgattt
    23161 tcttcctgat taaagaaacc ttaccaccgg agcgtcgtca gccatttcac attcgtacca
    23221 ctattggtaa ctttgcggcg ctgttccgtc ataagcgtgt cctgagttat atgctggcca
    23281 gcggtttcag ctttgccgga atgttctcgt tcttaagcgc cggaccgttt gtctacattg
    23341 aaattaacca cgtcgcgccg gaaaactttg gttattactt tgcactaaac attgttttcc
    23401 tgttcgtgat gaccattttt aacagccgct tcgttcgccg cattggcgcg ttaaatatgt
    23461 tccgctcggg gttgtggata caatttatta tggcagcgtg gatggtcatc agtgcgctgc
    23521 tggggctggg attctggtcg ctggtggttg gcgttgcggc gtttgtgggc tgcgtgtcga
    23581 tggtgtcatc caatgcgatg gcggtcattc ttgatgagtt tccccatatg gcgggaacgg
    23641 catcttcgct ggcaggaacc ttccgttttg gcataggggc aattgttggc gcattgcttt
    23701 ctcttgcgac ctttaactct gcatggccga tgatttggtc aattgcattc tgcgcaagca
    23761 gcttcattct cttctgtctg tacgccagtc gaccgaaaaa acggtgatct attgcacaac
    23821 gaggaagcta aaaggcttcc tttgttgatg catgtcaacc acaaatctat cattcccccg
    23881 atatatgttt attttatgta aaatcaattt atgtaaaaag tcacatcatt gtagttaaaa
    23941 aggttgagtt agatcgcaga aacgggtaca tatagccccg caaacgtgac cacgcccgca
    24001 gatattactt aaatcagagc catagaggcc acgcaggcga ggcatcaatc tttacgatct
    24061 gtataaagac ggattgttga tgatgtgtta aaattgatgt aaacaaattg tgaagtgaat
    24121 gtacttccgg ggaaaataag tgacttcatt acaactctca atcgtccatc gactgccgca
    24181 gaactatcgc tggtctgctg gtttcgcagg ttcgaaggtt gaaccgattc cgcaaaatgg
    24241 accgtgcggt gacaacagcc tggtggcgct taaattgctt agcccggatg gtgataatgc
    24301 atggtcggtg atgtataaac taagccaggc gttaagcgac atcgaagttc catgttcggt
    24361 gctggagtgt gaaggtgagc cgtgcctgtt tgtaaatcgc caggacgagt ttgctgcaac
    24421 atgccgattg aaaaattttg gtgtggcaat tgccgaaccc ttttcaaact acaatccttt
    24481 ttaagcgtca gctcaacgcc agtagctgac gcgtatactc ctgctgcggt gtggcaaaca
    24541 cgcgcgcgca cggtccttgt tccactactt ccccttgtcg cagtacgata acctgatgac
    24601 ataacgcgcg gacaacgtgc aaatcgtggc tgataaacaa ataggccagt tgatgctttt
    24661 gttgcaatga tttcaatagc gtcaatattt gcgcctgtac cgttttgtcg agtgatgatg
    24721 tcggttcatc aagtatgatc agcgagggct taagaattaa tgccctggca atcgcaatac
    24781 gttgtcgctg accaccagag aactccgccg gataacggtg gcgtgtttca ggatctaatc
    24841 ccacttcatg catcacggct atcacttgtt gttcgcgttg tgcggcagaa agcgtcggct
    24901 ggtgaacccg taagccttcc tcaataatct gcaaaacgtt gagtcgtggg ttgagcgagg
    24961 agtttggatc ctgaaatacc acctgaatgc gatgacgaat aggtaacagc tggcggcgat
    25021 ttaaattttg cagtggctga ccgtcaaaga tgatgctgcc ctgagaatta atcagtcgca
    25081 gcagcgccag tcccgtcgta cttttcccgg aaccggactc gcccactaaa cccagtgttt
    25141 cacccgctcg tagcgtaaaa ctgatgtttt tcaccaccac attatgatcc acaatgcgct
    25201 tcaaaatccc tttgcgaatg gggaaggcaa cctgaagctg ttcaacatcc agcaacgttg
    25261 aggcaggttc tggcaacggc actggatcgc ctgacggttc actgttgagt agcttttgtg
    25321 tgtaaggatg agtgggtgat gcaaatagcg tagcggcgta attttgctcg acacagcgac
    25381 cgttttgcat taccgccacg cggtgggcca gttttctgac aatgctgagg ttatgagtaa
    25441 taaacagcat gcccatattc agctcgcctt gcagttcgcg caacagctgt aaaatctgcg
    25501 cctggacaga gacgtccagt gcggtggtcg gttcatcggc aattaataat tccggtcgcg
    25561 ttaacagcgc catcgcaatc atcacccgct ggcgttcgcc gccggagagc tgatgcggat
    25621 aatctgtcag ccgttttgcc gcctggcgga taccaacgcg atcaaggcag ttaagaattt
    25681 cgccacgagc cgcttcccga cgcatcccgc ggtggagtga aagcacttca taaagctgtt
    25741 tttccagggt atgcaatgga tttaacgaca ccatcggttc ctgaaaaatc atggcgatct
    25801 tattaccgcg tacaccgcgc aacgtttgat cgctgacatg aagcagcgat tcgccatgaa
    25861 aacgaatatc gccggagaga tattcaaccg gcggggaagg gagcaggcgt aaaattgaca
    25921 gcgcggtaac gcttttgcct gaacctgact cacccaccag cgccagcgtt tcgccagcct
    25981 caatctgtag tgaaacatca ttgactactg tacgtacggt ttgctgatgg cgaaaaccca
    26041 ccgacaaatt ttcaatcgct aacagagttt gcgtcatgct acaccgcctt attaggatca
    26101 aatgcgtcgc ggacggcttc accaataaag atcagcaaag acaataatat cgccaccgac
    26161 aagaaggcgg tgatcccaag ccacggggcc tgaaggttat ttttcccttg taacagcagt
    26221 tcaccgagtg acggtgaacc gagcggtaga ccgaagccga ggaaatcgag cgaggtcagg
    26281 gtggttatcg aactacataa aataaacggt aaaaaggtga gggtcgcgac catggcatta
    26341 ggcaacatat gacgcaggat gatactgcga tcgctgacgc caagcgcctg tgccgcacga
    26401 atgtagtcga aattacgagt acgtaaaaac tccgcccgca ccacgccgac cagactcatc
    26461 cagccaaaca agacagtaat tgccagcagc caccagaagt taggctgtac gacgctggaa
    26521 agtaaaataa tcaaaaacag cgtcggcatc cccgaccata cttcaataaa gcgttgtccc
    26581 cagagatcga ctttaccgcc gtaatagcct tgtagcgccc ccgccagcac gcccatcacg
    26641 ctggaacaga gagtcagcat caggccaaac agaaccgaga tccgcgtgcc atagagaatg
    26701 cgtgccagca catcgccgcc gttggcatcc gttcccagcc agttttgccg ggagggggga
    26761 gaagggaagg gcttatcggt agcaaagttg atactggtag caccaaagcg aatcggtgcc
    26821 cacagtaccc agccgttatt ttccagccgt tgtttcagcc acgggtcctg ataatcagcc
    26881 tgacttgcca gcgggccgcc aaaatcgctt tcgctgtagt ttttcaacaa cgggaaatac
    26941 caactgccgt cataacgtac cagcaacggt ttatcgttgg cgataagttc agaacacaaa
    27001 ctcaaaccaa acaaaacgag gaaaatccat aacgaccagt agccgcgacg gttatggcga
    27061 aaacgcgccc aacgggcctg attgacgggg ctgagtcgcg acattaacgt ccctcaaaat
    27121 ctatacgcgg atcgaccagc gtatagctga tatcactgac aatattcagc agcaggccaa
    27181 tcagggtgaa aatatacaag gtaccaaaca ttacaggata atcgcgcgag acggtcgctt
    27241 cgtagcccag taagccaagg ccattgagtg aaaacatcac ttcaatcagc agcgagccgg
    27301 taaaaaacat gctgataaac gtcgccggaa aaccggcaat caccagcagc atggcgttgc
    27361 ggaacacatg tttccagaga atattttttt cacttacccc tttcgcacgc gcggtcacca
    27421 cgtattgctt gcgcacttca tcaaggaatg agtttttggt cagcatggtc agcgccgcaa
    27481 agccaccaat cactgtcgcc agcaccggca gcgtgatatg ccacagataa tcggtgattt
    27541 tctgatacca cggcagcgaa tcaaagttag cggaaaccag gccgcgtaga gggaacaggt
    27601 cgaaatagct gccgcccgcg aagaagacaa tcagcaggat ggcaaacaaa aaggccggaa
    27661 tggcgtagcc gatgatgata aatgcgctac tccagacgtc aaagcggctc ccattataaa
    27721 cagctttgcg aatgcctaac ggaatcgaca ccagatagat aatcagcgtg ctccacaatc
    27781 cgagggtgat ggaaaccggc agactgtctt taatcagcgt cagcaccgag gcgctgcgaa
    27841 aaaggctatc accaaaatca aagcggatgt agtcccagat cattttgaag taacgttcgt
    27901 ggatcggctt atcgaaaccg tagcgatgag tgatctcggc gatcacttct ggatctaatc
    27961 cgcgtccgcc acggtaatta ctgtcgctga tattgccgac gcccgtttgc gcatggctgg
    28021 cgcgaatacc ttcaccgcct gcgccgggta atactccggc attaccaaac tcaatggcgg
    28081 cgatggcctg gtcgaccgga ccgccaggcg caatttgcac gatgaaaaag ttgatagtga
    28141 taatcgccca taacgttggg atcaccagca acagacggcg aatcagataa gcgcccatct
    28201 actctccctg ttgtctggct gacggcagtt tggccgcttt attaacgtca taccaccagg
    28261 tatcgatacc caggctgtaa acagggcgta cagcgggttg ggagaattta tcccaccagg
    28321 cgagacggtc ttccgccatg taccacattg gcagcatgta ataattccac gttaatacgc
    28381 gatccagtgc tcgccctaac ggcagtaatt tttctttatt tccctgcgcg gcaataattt
    28441 gattgatcag cgagtcgata accggacttt gcacgccggg ggcattataa gtggaattga
    28501 tatattccga tgaccaggaa atctgtaaat cggaactggg ccacggcatc gcccgccata
    28561 cgcgcggcat catgtcatag tcgcgactgc gcatgcggtt ggtgatttgc gagttatcca
    28621 ccttgcgaat gtccatgttg atacccagcc gttgcaggct gtgctggaac ggcaataccc
    28681 actgactatt gctgcttgcg ggaagcaata attcaaagct gagtggctga cccgttgtgg
    28741 cattaacgcg ttgctgaccc ttcagcaccc agcccgcttc gttgagaagt ttgtcggctt
    28801 ttaacaggtt gtcacgatcg tagccatcgc ctttagatac cggcggctgg tagatttgtg
    28861 tgaagacttc tgacggtaga tcttttttca ttggtgccag aagcaccagc tccgcggcgt
    28921 cggggtaatt tctggccgcg tattcggtat tctgaaaata actgttcgtg cgactccagg
    28981 cattgtaaaa caacgccttg ttcatccatt caaagtcaaa ggcgagggtg attgcttccc
    29041 ggacccggcg atcgctgaat accggacgtt ggatattaaa cgccagccag cgcgtatcct
    29101 gggctgattc gttcttttgc tcatctttga tgatgtattt tttatcgaaa tttttaccgg
    29161 tataacgcgt ggcccagttt ttagcgtcgt tttccatacg caaatcaaag gcacctgctt
    29221 taaacgcttc aaaggcgaca ttatcatcga ggtaataatc gtagcgaatg gtgtcgaaat
    29281 tccagcgtcc acggtttacc ggtaagtttg ctgcccagta atctttcacg cgggaataga
    29341 caatattttg ccccattttc caggacgtaa ttcggtacgg accactggca agcggaggcg
    29401 tggcgagcgg atcgctaagt ttgtgatcct tccagtactt ttctggaaat accggcagcg
    29461 aaaacagact caacatatct tctttgccgg gtttagctaa ctcaatgcgc acggttaacg
    29521 gcgcaatggc tttgacggtg gtgcctttgt agaccagacg aaattgcggc acgccttcgg
    29581 tcataaattt ttgaaaagta aactctacat cgcgggcagt aatgggcgaa ccatcatgaa
    29641 aacgggcgcg cggattaata gcgacctcca cccaggaata atcgtcagca tagcgtgcgc
    29701 tttcagcaat cagcgggtaa taactgcctg gttcgtcatc ggaagtcgta aatagcgtgt
    29761 cgtacagctg ctcggtgcgt gcgcccgggt tgccgcgcag tgcatagcgg ttgaaattat
    29821 cgaaggtacc gagggctgac aacgttatct gcccaccttt tggcgcggcg gggttcacat
    29881 aatcaaaatg gttgaaatta aacgcgtacc ggggttcgcc cagcacggca aaggcatagc
    29941 tttccttgat agcctgcgcc tgcgcaccaa aggtgaacag agcgataaac agcagcagta
    30001 tgcgcacaat catctgcaac ggatttcctt ttactcatca ttagccgcaa tccggcatgc
    30061 tttcactatg aataataagg gacgtaagca caccttacca ctgcggcgta tacggtttct
    30121 tcagccagcg aacaaaatcg tccagcggta acgggcgact aatccagtag ccttgcatga
    30181 aattaacgcc gcgttcgctt agccatcgcg cttgttccgg cgtttcgacc ccttcagcaa
    30241 ccgtcagcat attgaggcgt ttcgccagcg tcagcaccgc gtcaagtacg ggtgaagtga
    30301 tcgtttccgt accgatggcg ttgataaatc cacggtcaat tttcagataa tcgagcgtaa
    30361 aacgctcaag atagataagc gcgctgtgcc cggtgccgaa gtcatcaata gcaatttcta
    30421 cgcctaccga gtgcagccag gcgaagagtt gtgtggcttc ttgctctttc agcatatcgc
    30481 gctcggtaat ttccagcaca atctggaaat ggtgtgcggg tagggaagtg agcagtttct
    30541 ggatatctgc tttaaagctt tcgctgtgca gatggtccgg cgcaatgtta atgccaaatt
    30601 tgacgcctac cggcagcact ttttctaatt ctgcggcatc gcgggcaatt aactcaaaca
    30661 gatgctgagt cagcggcaca atcatctttt gcgattcggc aaagttaatg aaggcatccg
    30721 ggggaatttc tcccgcgaca ggatgccgcc agcgtagcag tacttccagg cccgttactc
    30781 gcaaagcttg tgtatccacc accggttgat acgccacgta aaattgttcg cgcttgatgg
    30841 cggtcatgat ttctctgccg gggcgcatac gtacgctcat caggtaatag cagagcaggc
    30901 caacgacagt tccactcatg ccgcccagta aaaatgcgta ccacacatcg ttccatgtcc
    30961 agtcatctgc ataaagccga acggtcagag gaatgcccgc aattttagtt tcacggactg
    31021 gcatgtcggt taattcgtta acgttcatca aacgtgaaga aaaggtagat agcgcagtat
    31081 tgccaataat gagggcgacg ccatcgtaat cttcctgccg tgaactataa aagagtgaag
    31141 gcgtcaggtt gagattcaga gcggcaaaga cgccgctatt tttcagcaaa gggttgcgat
    31201 accagatgac gattgcgggt ttgttcggca ccatcggcgt gccgggtaag atcgccatat
    31261 cgacattttt attaatgtcg agcgccggaa tcaattcatt gagtggaatg tccatctcac
    31321 cggtcgcaga tgaacagaat gtttttttat ctttcaccag cacaaacgta cggacattca
    31381 tgctaaacgc cgcgcgggcg gtcagttcgg ggttagcctg ctggcaggta tctaaggtca
    31441 gcggctggag ccggtcagta gtggatttca ggtcggcaaa ataggtatcg agatactttt
    31501 gtacgtcggt aatcagtgtg tcgtatttga cttcgtgctt atgccaggcc actaaaaact
    31561 gaaggcaact caccagtatc gcaatcatca cgcctgcaac aatgcaggta agcaggagct
    31621 tacgtccaga attgggagcg cgtatgaaca tgattccgtt aatttcctga aattacgaca
    31681 acatttaaaa gataacaatg accggactat agcctggatc aagacgcgga aacatcattt
    31741 tctctgatga atgaaaacaa aaaagcactg cctaagcagt gctttcagtt aactcgttag
    31801 gatagccaag ggattgcatc caaacggttt attagctgcg gctgagaatc cggcgtgctt
    31861 cgttgtaacg cttcttccag tacggttcat tcatgctgga aataataaca ccactgctgg
    31921 tggaagcatg gacaaactga ttgttgccga tataaatacc gacatggcgt cccgttgaac
    31981 cggcacggaa cagaactaaa tcacccgtac gcaaattact gcgggaaaca gatttaccca
    32041 tttcctgctg ttcgtaagtc gaacgcggaa gttctaagcc aaattgctca cggaatgtac
    32101 gctgtacgaa accagaacaa tcgatacctt ttttagtgct gccgcccaga cgataacgta
    32161 cgcctttcca gtcagcatac tgatccataa ttcgcgattt tacgtcgaca ttacgaacca
    32221 ggttttcaaa ttcatcctga gaagcttgca gtgatgatgt ttcactaccc actgcacgtg
    32281 tctcaggatg catattcttt gcggtgttat ttgcactaca tgcagaaagc agaaccgcta
    32341 ctgcaatcgc ggggatcccg cgcaagatat atctcaaaat cggttgagat ttgaccatgt
    32401 tgtttgtttt cccttgaagt ccttaacgac aaatatcgtt ataaaaaaat gccaagcgac
    32461 acgagactaa ttacctgggc atgttgaaac aattccttaa ggccaaatct gtggactaac
    32521 gcacaaaatt atatgctgtg aggataatct accgccgagg caaaacgagt tcgagattac
    32581 ccgattggtg cggtcttggc gagcggtttt acatgatttt ttataagaaa aagtgataca
    32641 gaaagttaat aagcgggatt ggtcaattaa agtacgattg agtgaaacgc atgcaatcca
    32701 ttcatttact tagagaatga tttttgcgcg cgatggatga aagcacggtg cgcatcatca
    32761 ggattatcct cactataaaa ataagcctga tgatgttaat tactgtgagt tatttgtttt
    32821 ggaaatgttt gttttttccc ggtagtgatt tgtcaaaaaa ggtgattaat cgatcactta
    32881 atggcgtcag caacacccag ggcaaaccga tcaatatcac ggtcatcgaa ccgacaatga
    32941 tgtcagtaaa ccagtgtgcg ccaatcatta ctctgggaaa tgcaaaaacc acaaaaataa
    33001 taagggcgat aaggcctgca actttgccga aataacgcca catgaatgcc gaaaaaataa
    33061 gcagcatcat gccgtgatcg ccgggaaagc tatctcgtga ggcatctttc gtgggaacag
    33121 agagcagttc gctgacgcgg ttaatatcgg taaaagtcaa tgttgggctg gcccgtttta
    33181 caggaattaa tgcctgaccc agctggttta atagcactgc agttaatagc atgaccagac
    33241 ccataatcac gatacgtcgt ctgccagggg cgttttcttt cagccagaaa ctcagcatca
    33301 acattcccat cgccagcaga gaacaaccgt cgaaagcgcg attgttggtc aatgcaacca
    33361 gccacaaaaa ggccttactt tcgaccagtt tctgattaaa gaaataaaaa atatccgcat
    33421 caatcggcaa ccagaatcca tgattaacgg gaatatacca ggaaagaaac agcgcgaggc
    33481 cgacaatatt caacaacact atttgcggca aatttttaat catattttct ctaattatct
    33541 tactgaaaaa cgcaggcaac cttacgcggt agtcgctaaa cgaagcttca acagcgcgct
    33601 ctgcaaagca ttccagtcag cttcgctgct ggaaatcagc tcaatacggc tgtccggtgg
    33661 cgcaacgttt tgcgtttcaa tgtgcaggtc atcgccctga cggttgattc gtaccagccc
    33721 ttcgggaata cgcagcacgc ctttgacgcg ttccaccggt gcaagtcgcg cccattccag
    33781 aatgccaatg gtgtcgaata ccgtatcagc gtcgaatatc cagccgcagg cctgatatcc
    33841 ttgcccgctg ttcagactgc gacgccagcg ttgatgctct ggcaggctta acgctgctaa
    33901 cccttttttc acgacatgct gatgagaatg cgcggcgctg gcgggcaact cggctaaatt
    33961 gcgacgcggc aaatccagaa gatgaccgtc aactttccca tgctcactgt gaattaattg
    34021 tcgatcgcca ccattttgct gccaccaacg ctgtagcgct tgctcacttt cgggcgtcgc
    34081 acggtcggat ttattggcga caatgatgtc tgcggcagcc agctggtcac ggaagttttc
    34141 attgctggcg cttttttcgt ccagcagcaa gcgcggatcg agaatgcaca aggtggcgcg
    34201 cagatctatc cacggttcat agactggtgc ggttaacaga tcgaggatct gtttcggatg
    34261 gcccagcccg gtcggctcta tcaacaagcg gtctggtttt ccctgacgca gtaaggtatt
    34321 caaccctacc tgcatgggta aaccattaac gcagcacatg cagccgccgg ggatctcttt
    34381 cagcaatgcg ccgctatcgg cgagcaaagc accatcaatt ccgacttccc caaattcatt
    34441 aaccagtacc gcccattttt cgttgggatc tttatgggct aacagatgaa gaatcgacgt
    34501 ggttttcccg ctgccgagaa aaccggtgat gaggttggtc ctggtcacaa cggctccgca
    34561 atactctggt aagttaactg agcgttaatc ctggctaaaa aaggcagaaa agagaagggg
    34621 agagcggacg aaggtgagct tcatccgctt taaccgcaga aatcgttaaa ttttgagggt
    34681 gtttaatagc gcctgatgtg cgccgaattg tacgatttgt tgccaggctt gttcgatggc
    34741 ctgcacaaaa tgcgggttat ctggcagatc atcaccgaaa acttcacgca gggaaagcag
    34801 ggcggttacg cgttgttcac tgctgctgcc cgcaacaagt tcgcgaattt tatcgctaag
    34861 cggatcgcga acatcaatgg catttccggc atcatcaacg ccgctgacgt aacgcatcca
    34921 gccagcaacg cctaatgcca gcaacgacca gtccgtttcg caccccagat gtatgcgaat
    34981 acctgccagc atacgttgcg gtaatttctg gctaccatcc attgcgattt gccaggtctt
    35041 atgtttcagc gccggattag caaaacgtgc aattaacttg tccgcatatt gtgttaaatc
    35101 gacatcttta atttgtagtg tcggcgcttg ctcattcagc attaatgttc tggcggcatg
    35161 gcgaaatgcg cgatcctgca tgcaatcact gatatgggcg aatcctgaaa ggtaacccag
    35221 ataagcgaga aaagagtggc tgccattaag catacgcagt ttcatctctt cccatggcag
    35281 gacatcattc accatttgta cacctgcgac ttcccaggca ggacgcccag cgacgaagtt
    35341 atcttccacc acccactgga taaacggttc gcagctaatc gcgcagggat catttacccc
    35401 cagatgctgg ctgatttctg ccagtgattc gtcggttgca gccggaacaa tgcggtcgac
    35461 catggttccc ggaaaactga cgtgttattt tatccacccg gcgagttctg gcgaacgttt
    35521 ttctgccatt cccagcaccg cgtttttcac cacatggcca ttgtcgggaa tattgtcgca
    35581 ggagagcacg gtaaacggtg taaggcctcg ctcacggcgg cgtttcagtg cttcgacgag
    35641 aatacccggt gcggagtgag gttcttcagg ggtttgtaga tcgtgaataa tccgcggatt
    35701 actggtgtcg agtgcaccgg tggccgggtc aatacaatag cctttttcgg taatcgtcag
    35761 ggaaacaatt gccacctgtg gctcgcaaaa tttctcaata attgccgcta aggaatccag
    35821 ttttgcatta aggcattcgt gaacggcacc gacaattatc acctgattgc cgtccgcacc
    35881 tttttccagc acggtatata aatggttctg tgcgcggagc tggctcatca gttgatcacc
    35941 gctgaacaag ctgatttcac agatccccca gtcgccgccc tgggcattca gcacacgatc
    36001 ggtcagtaac gcctgatgag cgcggtgaaa ggcgccaaag ccaaaatgaa cgatacgtga
    36061 ttgcaactgc tggcgatcgt agcgtggagc gtgtatatga tgcgggagcg taacggaggc
    36121 aattgtcttc ataggagtca cgtcttagta acgataaatt aacaatgaca gtgtaaagtt
    36181 atatgacagc aattgaaatt ggtctggatt gttaatgcga tgaagtgtga gggcgatcaa
    36241 tcaacgagtg aaaattgagg gcgtaaggtg ttcaggaagc gcaaaataga agattggtat
    36301 aataaattaa aaaagggaca gcctgagcta tcccttttct gcatgcggct gaagttagtc
    36361 agcacgcccc atataacggc gttcttcgat atggatacga attttttcgc ccgggcttaa
    36421 gtattctggt acctgaatca ccagaccagt gctcaatgtc gccggtttgt tacgggcgct
    36481 ggcagatgcc cctttgatgc ctggtgcggt ttcaacgatt tccagatcaa cggtctgcgg
    36541 cagctcaagc gccagcagtt gaccgtccca ggtcagcacc tgcatgtccg gcatgccgcc
    36601 ctctggcata aacagcaact cttcttcaat ctgatctttg gtgaaggtat acggggtata
    36661 gtcttcttta tccataaaga catattcgtt gccatcgaca taggagaagt caacgtaacg
    36721 gcgggtcagc gtcacggtgt caacgatatc atcacctttg aaacgctctt ctactttcag
    36781 cccggtacgg acatcagaaa aacgcatttt gtacagcgta gcggcaccgc gggcagtggg
    36841 cgactgaata tcaatatcct taaccagcag cagtttgccg ttgtaattca gtaccatacc
    36901 ttttttaatt tcgttcgctc ttggcatcga aaaattcctg tagtcgaaaa tgtcaaaaat
    36961 atcgcgacaa agtactcgcg acgggccttt cgggcaagtg gtattcgcac ttttgctggt
    37021 gcaaaaaagg tggtactgtg cgcgctcatc aatccggtgg ttaaccttaa gagaacaacg
    37081 tatgaaatgc cgtccgggtt gcggggcgtg ttgcactgcc ccttcaattt ccagccctat
    37141 tcccggtatg ccagatggca agcccgccaa tacgccctgc attcagcttg atgaacagca
    37201 acgctgtaaa atattcacct cgccactgcg accgaaagtc tgtgcaggct tacaagccag
    37261 cgctgaaatg tgcggtaact cgcgtcagca ggccatgacc tggcttatcg atctggaaat
    37321 gctgaccgcg ccttaaacat ctttaatccg cagtaagcaa aacagagtgg cgataatcat
    37381 caccatcgca aaccagaaca cagcgtgata attccagatc tcggcgacga tgcccgccac
    37441 tgatcctgcg atgatccagc ccacgcgcga cgtgttggta tagagcgtgg tggctgaacc
    37501 cgcctgaccg ggcatcagat cctgaaaata gagcatcccg atgccgccca gaatgccaat
    37561 aaaaatagca tttagcagct gcaagcccaa cagaatgaca ggtgaatgcg ccatcagcat
    37621 tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc attaagaaac gcttacccag
    37681 acgtttggcg aaatatccgg caatcaacat cgtcgggatt tccagcccgg cggcggtccc
    37741 catcacaccg gccagtttct cgggaagatg cagttcgttg ataataaata gcggcatgtt
    37801 gatgatgtac aggctgttcg agccccacat caatgtacaa atgacaaaca gcagcagcgt
    37861 atcgcgacgg ttacggcgcg gcgcttcgat cgtgccggtc gccagcggaa gctctttttg
    37921 catcgacggt aaaaacagcc acaccatcac accgcaaaca ataaacgcta ccgctgcgct
    37981 cagatacatt accgtaaagc tgaaacccat cgctaaggca taagccagcg gtgggccaat
    38041 gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg aacatcaccg cctcacgtcc
    38101 ggttttgtcg gcatgttcac gggcaagggc aaacatttgc gggttagcgg tcgagccaaa
    38161 gctgctaaga aagacgccaa cgaatagcaa aacaaagtag ttgcgattcc aggcaaaaag
    38221 ggtgcaggcc agcacgccta acaggcagca aaagacaatc agcgatttgc gatcgccgcg
    38281 cttatcagag cgcccggcga gaaactgact taccagaatc ccaatgacag cgctgccggt
    38341 aaagaagaat cccaccatcg ccggacgagc atgtacttca tcagtaagaa aaatactgag
    38401 tgtcggggtt tgcagagcgc ccgcaatacc ggtgagaaag gcaacgatta aaaacgccgt
    38461 cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg gagttatgca tgctggtcga
    38521 tcactggaag aggaagacgc ggagtttacg ctgcttgtca gaaaataaac agacagcgaa
    38581 atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt tgctgattgg ctgctgacgc
    38641 tgctgaaatg aaattgctga taacggattt tcccatcagc aattaggaaa aatggcaaaa
    38701 aattgtgcag cacatcaaac ttttgctcat aactttacgg ctttccttgc gtgctgaaac
    38761 agaagtatta tgctttcttg aaacgtttca gcgcgatctt gtctttaacc ctaagccagg
    38821 ttggcgcttt ttttctcata gaggctgaat cgtttcaatt cagcaagaga ggagaactat
    38881 gttccagtta tccgtacagg acatccatcc gggcgaaaag gccggagaca aagaagaggc
    38941 gattcgccag gtcgctgcgg cgctggtgca ggccggtaat gtagcagaag gctacgtcaa
    39001 tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc ggcaatggta ttgctattcc
    39061 acacggcact accgacaccc gcgatcaggt gctgaaaacc ggcgttcagg tatttcagtt
    39121 cccggaaggc gtcacctggg gtgacggtca ggtagcgtac gtggcaatcg gtattgctgc
    39181 cagctcggat gaacatctgg gcctgctacg ccagctgacc cacgtactga gcgatgattc
    39241 cgttgctgaa caactgaagt cagcaccaac agcagaagaa cttcgcgcat tactgatggg
    39301 cgaaaagcag agtgagcagc tgaagctcga caacgaaatg ctgacgctgg atatcgtcgc
    39361 cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt ctgaaagagg cgagggcagt
    39421 tgacgccact ttcgtcacca aagccatcaa tgaacaaccg ctgaacctcg gacagggtat
    39481 ctggctgagc gatagcgccg aaggcaatct gcgtagcgcg attgcggtaa gccgtgcggc
    39541 aaatgctttt gatgtggacg gcgaaacggc agccatgctg gtgagtgtgg cgatgaatga
    39601 cgatcagccc attgcggttc ttaagcgtct cgctgatttg ttgctcgaca ataaagctga
    39661 ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg ctgaccagcg atgatgcgcc
    39721 gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat gaacacggcc tgcatgctcg
    39781 tccaggtacc atgctggtca ataccattaa acaatttaac agtgatatta ccgtgacaaa
    39841 ccttgatggc accggcaaac cggcaaacgg acgtagtctg atgaaagttg tggcacttgg
    39901 cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt gcagatgctg aacaggcgct
    39961 gaaagcaatc ggcgacgcta tcgctgctgg tcttggggag ggcgcataat gagcagacgt
    40021 gttgctacta tcacccttaa tccggcttat gaccttgttg gtttctgccc ggaaattgaa
    40081 cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg cggcgggtaa aggcatcaac
    40141 gtggccaaag tattaaaaga cctgggaatt gatgtcaccg ttggcggctt cctcggtaaa
    40201 gacaatcagg atggttttca gcaactgttc agcgagctgg gcattgccaa ccgtttccag
    40261 gttgtacagg ggcgcacccg aattaacgtt aagctgacgg aaaaagacgg cgaagtgacc
    40321 gacttcaact tctcgggttt tgaagtcacc cccgccgact gggaacgctt tgtgactgat
    40381 tctctgagct ggctcggtca gttcgatatg gtctgtgtca gcggaagctt accgtcaggc
    40441 gtcagcccgg aagcgttcac cgactggatg actcgcctgc gtagtcagtg tccttgcatt
    40501 atctttgata gtagccgtga agcgttagta gcaggtttga aagcggcacc gtggctggtg
    40561 aaacctaacc gccgcgagct ggaaatctgg gcaggccgta aactgcctga aatgaaagat
    40621 gtgattgaag ctgcacatgc gctacgtgaa caaggtattg cgcatgttgt tatttcactg
    40681 ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat ggatcgccaa accaccgtca
    40741 gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg ttggtggcct gatttatggc
    40801 ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg cgactgctgt tgcagccctg
    40861 gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc agttggccgc aatgatggcg
    40921 cgcgtcgact tacaaccttt taactgacag caggagaggc ataatgaaaa cgctgctgat
    40981 tattgacgct aatctcggtc aggcacgcgc ctatatggcg aagaccctgc tgggcgcggc
    41041 ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac gacgcagaaa tggcgattgt
    41101 tctcggtgat tccatcccga atgacagcgc gctgaacggt aaaaatgtct ggctgggtga
    41161 tatttcccgg gcagttgcgc accctgagct gttcctgagt gaagccaaag gccatgcgaa
    41221 accttacact gcgccggtca ctgcgacagc accggttgcc gccagcggtc cgaaacgcgt
    41281 agttgcggtg actgcttgcc cgactggcgt agcacacacc tttatggcgg ctgaagccat
    41341 tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt gaaacccgtg gttctgttgg
    41401 cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg gatctggtga ttgtggcggc
    41461 agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg atgtatcgta cctctaccgg
    41521 tctggcgctg aagaaaaccg cgcaggaact ggataaagcg gttgctgaag caacgccgta
    41581 tgaaccggcg ggcaaagctc aaacggcgac ctctgaaggt aagaaagaga gtgcaggcgc
    41641 ataccgtcac ttgctgacgg gcgtctctta catgctgccg atggtcgttg caggtggtct
    41701 gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt aaagagccgg gtacgttggc
    41761 agcggcgctg atgcagattg gtggtggttc agcctttgcg ctgatggtgc cggtactggc
    41821 aggttacatt gccttctcca ttgccgatcg tccgggtctc acgccgggtc tgattggcgg
    41881 tatgctggcg gtcagcaccg gttctggctt cattggcggt attattgcgg gcttcctggc
    41941 tggttacatt gcgaagttaa tcagtacgca actgaaactg ccacagagta tggaggcgct
    42001 gaaaccgatc ctgatcattc cgctaatttc cagtctggtg gtcggtctgg cgatgatcta
    42061 cctgatcggt aaaccggttg ctggcattct cgaagggttg actcactggc tgcagaccat
    42121 ggggactgcg aatgcggttc tgctgggggc gatcctcggt ggcatgatgt gtactgacat
    42181 gggcggtccg gtaaataaag cagcgtacgc attcggtgtg ggtctgctga gtactcaaac
    42241 ctatggcccg atggcggcga ttatggcggc aggtatggtg ccaccgctgg caatgggcct
    42301 ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag caggaaggtg gcaaagccgc
    42361 tctggtactg ggactgtgct ttatttcgga aggtgcaatt ccgtttgctg ctcgtgatcc
    42421 gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg actggcgcaa tctcaatggc
    42481 gattggtgcg aaactgatgg caccgcacgg tggtctgttt gttctgctga tccctggcgc
    42541 tattacgccg gtattgggtt acctggtagc aattattgcc ggtacgctgg tggcgggttt
    42601 ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta gcgaaagccg cgtaataaaa
    42661 ggtgtgttac agggcagaaa tttactgccc tgtatcattg gatgagcgca aagagaatga
    42721 ccggtttgtg aatgtaattt tatgaattta aatggtttta tctttcagta gaaacctgaa
    42781 acgttcatca aacactaagg ttatttcctc aagggcagat tcttttctgc ccttttttag
    42841 tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt tgtaattctg gcatttaatc
    42901 gcattttgtg agcctgctca aatctgcccg ctttgcctct ctggcggcaa tttactttac
    42961 gtaaaacatg catactatga gcacatgttt aaatatgaac acatgtttaa actgtctttg
    43021 cagtatctat attcttgtgg attgacaggg ttggtaattt tttaccagag gacgtgctat
    43081 gcgcgaaaag gattatgtcg taattatagg ttcggcgaat attgatgtcg ccggatattc
    43141 acatgaatca ttaaattatg cggattcaaa tccaggtaaa ataaaattta cacctggtgg
    43201 agtagggcga aatattgcac aaaacctggc gttgctgggt aacaaagcct ggttactgag
    43261 cgcagtaggt agtgattttt atggtcaatc gctgctaacg caaaccaatc aatctggcgt
    43321 ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg tcgagttatt tatcattact
    43381 cgataatacc ggtgaaatgc tggttgctat aaatgacatg aatattagca acgctattac
    43441 agctgaatat ctcgcacagc accgtgaatt tattcagagg gcaaaggtca ttgtcgcgga
    43501 ctgtaatatc agtgaagagg cactggcatg gattctggat aatgctgcca acgtacccgt
    43561 atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg cgcgaccgtc taaatcagat
    43621 ccacactctg aagccaaacc gccttgaagc ggaaacactg agtgggattg cgctgtcagg
    43681 gcgtggagat gtggcaaaag ttgctgcctg gttccatcaa catggcctga accgactggt
    43741 attgagcatg ggcggcgatg gcgtttatta cagtgatatc agcggtgaaa gtggctggtc
    43801 tgccccgatc aaaaccaatg ttattaatgt taccggagcg ggcgatgcca tgatggcggg
    43861 acttgcttcg tgttgggtag acggaatgcc gtttgccgaa tctgttcgtt tcgcacaggg
    43921 atgttcgtca atggcgctct cctgtgaata caccaataac cccgatttat cgattgccaa
    43981 cgttatatcg ttagtggaga acgcagaatg tctgaattaa aaatttcccc tgaattatta
    44041 caaatttccc cggaagtgca ggacgcttta aaaaacaaaa aaccggttgt tgcgctggaa
    44101 tcgaccataa tttctcacgg aatgccattc ccacaaaatg cccaaaccgc aattgaagta
    44161 gaagaaacta ttcgtaaaca gggcgcagta ccagccacta tcgccattat tggcggtgtg
    44221 atgaaagtgg gcttaagtaa agaagaaatc gaattgctgg gccgtgaagg acataacgtg
    44281 accaaagtta gtcgtcgcga tttacctttt gttgttgccg ccggaaaaaa tggcgcaacc
    44341 actgtggctt caacgatgat tattgcggcg cttgccggaa ttaaagtatt tgccaccggg
    44401 ggaattggtg gtgtgcatcg cggggcggaa cataccttcg atatttctgc cgatttacag
    44461 gaactggcaa ataccaatgt cactgttgtt tgtgcggggg caaaatctat tctcgattta
    44521 ggattaacaa cagaatattt agaaaccttc ggtgtaccgt taattggtta tcagacaaaa
    44581 gcgctgcctg cgtttttctg ccgaaccagc ccatttgaag tcagcattcg tcttgacagt
    44641 gcccgcgaaa ttgctcgcgc gatggcagtg aagtggcaaa ccggtttgaa cggcggcctg
    44701 gtagtggcga atccgatccc ggaacagttt gcgatgccag aagaaaccat caatgcagca
    44761 attgatcaag ccgttgctga agccgaagaa cagggtgtta ttgggaaaga gagtacaccg
    44821 ttcctgctgg cgcgtgttgc ggagctaaca ggcggtgata gcctgaaatc caatattcaa
    44881 ctggtcttta ataacgccat tctggcgagc gaaattgcca aagaatatca gcgtctcgtg
    44941 ggttaatttc tggcgggcag aaagccgtcg cccgtactca tcaaacatac caaaacctgg
    45001 cgctgacgcc gggtttactg gcatgaaggg aaaatcatta tggatataat gagaagtgtt
    45061 gtggggatgg tggtattgct ggcaattgcc tttgtgttgt cggtaaataa aaagagcatc
    45121 agcttgcgca cggttggcgc tgcgctgctg ctgcaaattg ccattggtgg catcatgctc
    45181 tattttcccc cgggaaaatg ggcagtagag caggcggcat taggcgtcca taaagtgatg
    45241 tcctacagtg acgcaggaag tgcctttatt tttggttcgc tggtcggtcc caaaatggat
    45301 gttctgtttg acggtgcggg ctttatcttc gcctttcgcg tactcccggc gattattttc
    45361 gttaccgcgc ttatcagctt gctgtactac attggcgtaa tggggctgct gattcgtatt
    45421 cttggcagta tttttcagaa agccctcaac atcagcaaaa tcgaatcttt tgtcgcagtc
    45481 accactattt tcctcgggca aaatgagatc ccggcgatcg ttaaaccttt tattgatcgc
    45541 atgaatcgca acgagctatt tacggctatt tgtagcggca tggcgtctat tgcgggttcg
    45601 atgatgattg gctatgccgg aatgggcgtg ccgattgact atttgttagc cgcttcgctg
    45661 atggctattc ctggcggtat cctgtttgca cgtattctta gtccggcgac ggaaccttcg
    45721 caagttacgt ttgaaaatct gtcgtttagc gaaacaccgc caaaaagcat tattgaagct
    45781 gcggcgaacg gtgcaatgac cgggctgaaa atcgccgccg gtgtggcgac ggtggtaatg
    45841 gcatttgtgg caattattgc gctgatcaac ggcattattg gtggagttgg cggctggttt
    45901 agttttgcca atgtctctct ggaaagcatt ttcggctatg tgctggcccc gttggcatgg
    45961 attatggggg tggactggag tgatgcaaat cttgccggaa gtttgattgg gcagaaactg
    46021 gcgattaatg agtttgttgc ttatctcaat ttctcgccct atctgcaaac gagcggcacg
    46081 ctggatgtca agaccattgc cattatctcg ttcgccctct gtgggtttgc aaactttggt
    46141 tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc caaaacgtgc tccagaaatc
    46201 gcccagcttg gtttacgggc gctggcagca gcaacgcttt ccaacctgat gagtgcgact
    46261 attgccgggt tctttattgg tctggcgtaa tgctcgattt gtagaccgga taaaacgtat
    46321 taagcgttgc atccggcaat cgcgctattg caaataattt ttactgcatc atcccggaga
    46381 atttattctc cgggtccatc tccagcgcca gtcctgacaa ctgctttctg tttttaatga
    46441 gatacccttt cttgctttta gttaataaac cgtcgtgaat gaattgcgcg agaacatata
    46501 aaagatgtcg ataagaaacg cctaaatact ctgccgcttg cgtatgcttt tcgtgataaa
    46561 gatcaccttc ctgcgagagt aatataaatg ctgccaggcg attcactaac ggaaatgatt
    46621 gattctgagt taaagaaaca atattacgat aatttttatg acttaaggtg acgcagagtt
    46681 ttcgtaaaaa tagcgtgtcg tttaataacc gcgggcggta atgtttcata gggagcgcaa
    46741 ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc atggtctttg tcgattaact
    46801 caatctcgcc aataaaacag ggggcggcaa agaaatcgat cagcgacacg cgaccattag
    46861 ctagcgtggc gtaaagcctg gcgcggcctc gcgtcaggta aaacagccag gacggctgtt
    46921 gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa cagccgcgta tcggctgaaa
    46981 catccgttaa aaagcaatcc ttaaacgcgg attcactcat caattgctgc ttaagatcat
    47041 tattgtggat ttctttcacg atgtttatcc gctatgagat ttctcatatt tagcatacgt
    47101 acgcggttgg taagattcaa tcatcggccc ggtcgcattt tctttgctgt agaaaaaata
    47161 acgcaactgg aaacagagga aataaacaat ggaaaagaga aaaattattc tggattgtga
    47221 tccgggtcat gatgatgcta ttgctataat gatggcggcg aaacatccgg caatagattt
    47281 attaggcatc actattgtag cgggtaatca gacgcttgat aaaacattaa ttaatggcct
    47341 gaatgtttgc cagaaactgg agattaatgt tccggtttat gcggggatgc cgcaacccat
    47401 tatgcgtcaa caaatcgttg ccgataatat tcacggtgaa accggactgg atggcccggt
    47461 attcgagccg ctgacccgcc aggcagaaag tacccacgcg gtgaaataca tcattgatac
    47521 cctgatggca agcgatggcg atatcactct ggtgccggtt ggtccgcttt caaatatcgc
    47581 ggtggcaatg cgtatgcaac ccgcgatcct gcccaaaatc cgtgaaattg tgctaatggg
    47641 cggcgcttac ggtacaggca acttcacgcc atctgccgag ttcaacatct ttgctgaccc
    47701 ggaagcagcc cgcgtggtgt tcacctctgg cgttccatta gtgatgatgg gcctcgatct
    47761 caccaaccag accgtttgca ccccggacgt gattgctcgg atggaaaggg caggcggccc
    47821 tgccggagag ctgttcagcg acatcatgaa cttcactctc aaaacgcagt tcgaaaacta
    47881 cggcctggct ggcggcccgg tgcacgacgc cacctgcatc ggttatctga ttaaccctga
    47941 tggcattaaa acccaggaga tgtacgtcga agtggacgtc aacagtggcc cttgctatgg
    48001 gcgcaccgtc tgcgacgagc tgggcgttct tggcaagccc gccaatacca aagtcggcat
    48061 cactattgat accgactggt tctggggctt agtcgaagag tgcgtgcgcg gctacatcaa
    48121 aacccattaa gcgttgataa caagccgggc cgatagcgca agatgcaggc ccggctattt
    48181 ctgatgggca gaaagccgtc gcccaatatg acatgaagag ataatcacta tggatgtcat
    48241 gagaagtgtt ctgggaatgg tggtattgct ggcaattgca tttttgctgt cagtaaacaa
    48301 gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg ttacaggttg tgattggcgg
    48361 cattatgctt tggttaccgc cagggcgttg ggtcgctgaa aaagtcgctt ttggcgtgca
    48421 taaagtgatg gcgtacagcg acgcgggtag cgcatttatc ttcggttcgc tggtcgggcc
    48481 gaaaatggac acgctgtttg atggcgcagg atttatcttt ggtttcagag tattaccggc
    48541 aattatcttc gttacagcac tggtgagtat tctctactac atcggtgtga tggggatttt
    48601 aattcgcatt ctcggcggta tcttccagaa agcgttaaat atcagcaaga tcgagtcatt
    48661 cgtcgcggtc actaccattt tcctcgggca aaacgaaatt ccggcaatcg tcaaaccctt
    48721 tatcgatcgt ctgaatcgca atgaattatt tacagcgatt tgtagtggca tggcctcgat
    48781 tgctggttcg acaatgattg gttacgccgc cctgggcgta cctgtggaat atttgctggc
    48841 ggcatcgtta atggcgatcc caggcgggat cttgtttgcc cgcctgttaa gcccggctac
    48901 ggaatcttcg caggtttcct ttaataacct ctctttcacc gaaacaccgc caaaaagcat
    48961 tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac
    49021 agttgttatg gcatttgtcg ccatcattgc gttaattaat ggtattatcg gcggcgttgg
    49081 cggctggttt ggttttgcac atgcctcgct ggagtccatt ttaggttacc tgttggcccc
    49141 attggcgtgg gtgatggggg ttgactggag tgatgcaaat cttgccggga gtttgattgg
    49201 gcagaagctg gcgatcaatg aatttgtcgc ttatctcaat ttctcgccat atctgcaaac
    49261 gggtggcact ctggatgcta aaaccgtggc gattatttct ttcgcgttgt gcggtttcgc
    49321 taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc
    49381 gccggaaatc gcccaacttg gtttacgcgc gctggcggcg gcgacacttt ctaacctgat
    49441 gagtgctact attgcaggat tctttattgg tttagcgtag tgcgcaattt atgagccgga
    49501 taagatgcgg tcaagcgtcg aatccggcaa cattgttaca ccattggcac taatgaaagc
    49561 gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgcga aattgccgcg
    49621 caggctaccg caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa gctatatacc
    49681 aagcccgcca taaaaccatc gtcagcacca aaactgtcta ctgtcgtatg cgctggcgcg
    49741 gtcagcaaaa attgttctcc atccttatcg ctgcaaaata cagattcatc tttcagataa
    49801 acaaaaatgc gtttaacacc ttgttgatgc agcgaattta cagctctgat gcgatcgttg
    49861 tcgtctttaa tcggttgccc ccacaaaatt tccagttcat tttgtgttgg tttcagagtg
    49921 tggatccggc tgtaccagtt ttttactttg ttcgctttaa attcggaaac ggtatcgaca
    49981 aacatcggaa tttcatcagc aatggtaaag acccactcca gggcttcagg tgtgagatta
    50041 caatcagcca gcaccacacc agcatggcga atcaagtccc gtgaagtatt gagcagttga
    50101 ggcgttagct gttgcagaat atgtgtgtca ttgattgcca ggatagtttc ttcctgctta
    50161 ttagcgatag cgagataagt tgcagtgctg tgtccatgca gacgaatgca gtttgaaacg
    50221 ttcacgcccg cgcggcgcgt ttcttccagt aatgtctcgc cgtaaaaatc gttgccaata
    50281 gctgatatta aatgcacatc ccgaccaagc agagccaggt tatgcgcgat attacgtccg
    50341 acgccgcctg cggaacagtg aacagaacct ggatgagagg ctgcttgcgg gtaacggata
    50401 tccgccattc cgcgaatatc catattgatt gcacccacaa ccacacagta atcctgctcg
    50461 gtaaggatat atcctttgcc tttaatcaaa cccttgcgca tcaaatccat aatatgagcg
    50521 gcaacacgtg agcggctgat ttgcagaata tcggcgattt cattctgttg aatcagcggg
    50581 ttacgccgta aaattttaag aatctgcttt tccctgtcgt tcatcgatca ggccaccgct
    50641 ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc cgggttgatg
    50701 gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca gcggaacgca
    50761 tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct gccaaaggtg
    50821 cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccaacaat acgggcaaaa
    50881 tccgcgaacg ttttctcgca ttcggctgga gtacgcaaat catacccggc agcgaaagca
    50941 tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc gataatcgcc
    51001 gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc
    51061 gctgttacac cttgagtttt atccagcgca atgttgatgg attcggcaat acgcgcaagg
    51121 caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag caaagaaagc
    51181 cccagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt ttccagagct
    51241 tcagtgaccg gatgtccgag gttaatcaga tagctgtcgt ggggaagaat ttgcgccgat
    51301 gtgtagtgat atttttcaca ggccgctttg aattcatcga tggtttgcgt cgtgagcggt
    51361 gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg
    51421 gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc tccaatgtat
    51481 ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag cgggttaaca
    51541 ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa tcgcaccacc
    51601 accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg ccccggcttt
    51661 tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg ccagcaggaa
    51721 ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga acgagttaaa
    51781 gatggcaacg acgatgaaca atatggcaaa ccacggaata gtgattttgc ttttctcgcc
    51841 gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga acggagccag
    51901 catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg ggctgatggc
    51961 atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt agataccgaa
    52021 agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga ggaaaatcgc
    52081 gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg cttccgcttt
    52141 cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac cggcaccgat
    52201 caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc aagccagcag
    52261 gaaggtactg gacagcgtca gcacgtcaat gatgatccca ctgataccga catcggcaat
    52321 ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata aatattgctt
    52381 ggcaaacagc acaccaccgt cacagctttt ccagatgtgc ggatagatgg tgttgcctaa
    52441 aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg caaccgcggg
    52501 aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta accccggaat
    52561 aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa ccctctcctt
    52621 taaccgggca taaggttacg actaactggt ttaaaaataa aattgattat atatttataa
    52681 ttaatcttta taagtggtaa gcgactatgc acatcaccct ccggcagttg gaagtttttg
    52741 cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat
    52801 cagcagtgag cgcagccttg accgacctgg aagggcagct tggcgtgcaa ctgtttgatc
    52861 gcgtggggaa aagactggtt gttaatgaac acgggcggct gctctatccg cgtgcgttgg
    52921 cattgcttga acaggcggtt gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc
    52981 gtatctatgc cagtagtacc atcggtaact acattctgcc tgcagttatc gcccgttatc
    53041 gccatgatta tccgcagttg ccgattgaac ttagcgttgg gaacagccag gacgtgatgc
    53101 aagcggtgct ggatttccgc gttgatattg gttttattga aggaccgtgc cacagcactg
    53161 aaattatttc tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg ccgacttcgc
    53221 cgttggcccg tggtccggtc accttagagc agctggccgc tgcgccgtgg atcctgcgtg
    53281 aacgcggttc cggcacgcgg gagattgtcg attatctatt gctgtcacat ttaccgaagt
    53341 ttgagatggc gatggaatta ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt
    53401 tgggaattag ttgcctgtcg cgacgggtga ttgaagatca attgcaggca ggcacattaa
    53461 gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc
    53521 aaaaacacct ttccaacgcg ctacggcgct ttctggacta ttgcgatccc gcgaatgtac
    53581 cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga cgcagcgccc
    53641 tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccct gggcgatcat
    53701 gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct gctacaatcg
    53761 cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa accacagaag
    53821 cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc attggcggtt
    53881 ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg
    53941 gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg accagtctcg
    54001 gtgaactggc tgcatatatg ccggtttccg gttcgtttgc cacttacggt cagaactatg
    54061 ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg gcggtgacta
    54121 tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg gatacaccgg
    54181 gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac atctcagttc
    54241 gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca gttattgtct
    54301 ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag cctgcgggct
    54361 ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg
    54421 tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt gctgcaggcg
    54481 agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc tggcgaatcc
    54541 tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac accgatccga
    54601 gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg gtgttccagc
    54661 acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg gcggtgctgt
    54721 cagcgggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg
    54781 gtaaagcgcc gcacattttc gctaaactgt cgcgtggtgg cgtgccgcgt aatgcgctgt
    54841 atgcgacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc aaccagacgg
    54901 tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg ctggggattg
    54961 ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac attaacgatc
    55021 tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt ctgtgtctga
    55081 ttatcacttt gggccagaac tacgaagcgt tcctgaaaga taccattgac tggggcggcg
    55141 tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc tacaagctga
    55201 ttaaaggaac tcacttcgta cgttacagcg aaatgaagtt cccgcagaac gataagaaat
    55261 aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca attccatttc
    55321 cctgacaaat catgcggata taaaatttaa catttgggtt gataattgtt atcgtttgca
    55381 ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctgttccg gctttctggg
    55441 atgatcacct gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg ggacgtgaag
    55501 aagatgtgag cgataaccca ttttattttc gtagttacct catggagata tggaatgttt
    55561 aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg ctatttcttg tgcatggcct
    55621 gtgttagcgg tcgatgatga tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa
    55681 aacctcaaag atgcacccgc cagtatcagc gtcattaccc aggaagacct gcagcgaaaa
    55741 ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa
    55801 ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctacac cctgattctc
    55861 gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga tttcgatctg
    55921 aactggatcc cggtcgattc catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg
    55981 ctatacggct ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag
    56041 aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga tcgcggtgat
    56101 acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct gggaatgaaa
    56161 gcttacggca gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac gaccaccgat
    56221 accggagaaa cgccgcgtat tgaaggattc tccagccgcg acggcaatgt cgaatttgcc
    56281 tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg tcaggatcgt
    56341 gattccgact cgctggacaa aaaccgcctg gaacgccaga actactccgt cagccataat
    56401 gggcgttggg attacggcac cagcgaactg aaatactacg gtgagaaagt cgagaacaaa
    56461 aaccctggca acagcagccc gataacttcc gaaagcaata cggtcgacgg caaatacacg
    56521 ttgccgctga cggcgattaa tcagtttctc acggttggcg gtgaatggcg tcacgacaaa
    56581 cttagcgatg cggtgaacct gaccggggga accagctcca aaacgtctgc cagccagtac
    56641 gcgctgtttg tggaagatga atggcggatc ttcgagccgc tggcgctgac gaccggcgtg
    56701 cgtatggacg atcacgaaac ctacggtgaa cactggagtc cgcgtgccta cctggtttat
    56761 aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa agcaccttct
    56821 ctgttgcaac ttagccctga ctggacgagc aattcctgcc gtggcgcatg taagattgtg
    56881 ggtagcccgg atctgaaacc agaaaccagc gaaagttggg agctggggct ttactacatg
    56941 ggtgaagaag gctggctgga aggggttgaa tccagcgtta ccgttttccg taacgatgtg
    57001 aaagatcgca tcagcatcag ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac
    57061 tttgttggtt ttgagacggg cgctaacgga cggcgcatac cggtatttag ctactacaac
    57121 gttaacaaag ctcgtattca gggcgtggaa accgaactga aaattccgtt caacgatgaa
    57181 tggaaactgt cgatcaacta cacctacaac gatggtcgtg atgtcagcaa cggtgaaaac
    57241 aaaccgctat ccgatctgcc gttccatact gctaacggta cgctggactg gaaaccgctg
    57301 gcgctggaag actggtcatt ctatgtttct ggtcactata ccgggcagaa acgcgccgac
    57361 agcgcgacgg ctaaaacacc gggcggttat accatctgga ataccggcgc ggcctggcag
    57421 gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt
    57481 cgtgacgact acagctataa cgaagacgga cgtcgttact ttatggcagt ggattatcgc
    57541 ttctgatgag aagatgcccg gcgaaccggg cggactttca cttcagtaaa tactgcgcat
    57601 ggaagcgcag gtgatcctct ataaaagagg cgatgaagta gtaactgtga tcatatcccg
    57661 gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc agtaccgcag
    57721 gttgcaactg gtcggcaaga aactgatcat tatcgccctg atcgataagc gtcgggatcg
    57781 catcctgcgc gttactggca tacatcagcg cgcaactgtc ccattccagc catgcatttt
    57841 tgtcctcacc taaatagcgg ctaaacgctt tgattcccca cgggacgctg cacggattca
    57901 caattggcgc aaaggccgaa acgctggtgt atttacccgg atttttcagc gccataatca
    57961 gcgcaccgtg accacccatt gagtgaccgc taatggcgca gcggtcgctg acattaaatt
    58021 gcgactgaac cagcgccggt aattcatcgc gcagataatc atacatccgg taatgcgtcg
    58081 cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa tcgtagccat
    58141 cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg catcaccagt acaatcccca
    58201 gttctgccgc cacgcgctgg gcacccgcct tggtggtgaa gttctcgtca ttgcaggtta
    58261 atccggaaag ccagtacagc actggtggcg gggtgtgatc acgagggggt gggagaaaga
    58321 tactgaacgt catcgggcag tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc
    58381 cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc
    58441 aatttcttca tcataccgtt tattcaatgt gctgtgagta actttcactt ccgtatttgc
    58501 ataacgatgt tttaacatct gctgatgaaa ggcagcggca attacaataa ttatcgctgt
    58561 gaatactgga ttatgtgcgc cgcctcacgc acaataatca ggctgtaaat cagcttaata
    58621 actttgcccc cacgcagggc ggaggcgtca cacctgcagg agaaatcata aatgccatca
    58681 ctcagtaaag aagcggccct ggttcatgaa gcgttagttg cgcgaggact ggaaacaccg
    58741 ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca aaagccttat tgctggtcat
    58801 atgaccgaaa tcatgcagct gctgaatctc gacctggctg atgacagttt gatggaaacg
    58861 ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct ccggtctgga ttacgccaac
    58921 ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc
    58981 gatatcactc tgaccagtac ctgtgaacac cattttgtta ccatcgatgg caaagcgacg
    59041 gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg tatcgtgcag
    59101 ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct tattgcgcta
    59161 caaacgctgc tgggcaccaa taacgtggct gtctcgatcg acgcggtgca ttactgcgtg
    59221 aaggcgcgtg gcatccgcga tgcaaccagt gccacaacaa cgacctctct tggtggattg
    59281 ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca tcacaactga
    59341 ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat tttgttcgcg gcgtcgccat
    59401 tctggggatc ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg cttatctcaa
    59461 tcccgcctgg tacggtgcta ttacgccgca ggatgcatgg acctgggcat ttcttgatct
    59521 catcggccag gtgaaattcc tcacgctttt tgcgctgctg tttggtgctg gactgcaaat
    59581 gttgctgccc cgtggcagac gctggatcca gtcgcggtta acgctgttag tcttattggg
    59641 ctttattcac ggtttattgt tctgggacgg cgatattctg ctggcttacg ggctggtggg
    59701 cttaatctgc tggcggctgg tgcgcgatgc gccatcggta aaaagcctgt ttaataccgg
    59761 cgtcatgctt tatctggtgg ggcttggcgt tttgctgtta ttggggctga tttccgacag
    59821 ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa aatactggaa
    59881 gcttcacggc ggcgttgatg cgatcagtaa tcgtgccgat ggtgttggca acagtttact
    59941 ggcactgggc gcacagtatg gctggcaact ggctgggatg atgctcattg gtgcggcgct
    60001 gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc gtactggttt
    60061 tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc aatggcagct
    60121 ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc
    60181 gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat taagccgctt
    60241 taagctggtg ctggcaatcg cctgcgtcgg acggatggcg ctgaccaact atctattgca
    60301 aacgctgatt tgtaccacgc ttttttacca cctcggtttg tttatgcatt ttgaccgcct
    60361 ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct ctgttatctg
    60421 gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc
    60481 tgccggaccg gcaatatcta aaacatcaag ataacgatct ggatcacatt cgttaacaaa
    60541 acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat tcagtgctga
    60601 caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag tgaatatgat
    60661 caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct
    60721 caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga
    60781 gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta gcgacaccat
    60841 tggcgtggtg gtgatggacg tttctgatgc gtttttcggt gcgctggtaa aagcggtgga
    60901 tctggtcgcc cagcagcatc agaaatacgt gctaatcggc aatagctatc atgaagcgga
    60961 aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca
    61021 ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc ccggtatggt
    61081 gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg ataatctcag
    61141 cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta ttggttatct
    61201 ttcttccagc cacggcattg aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt
    61261 gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc cggacatgcc
    61321 gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta ccgctgtatt
    61381 tgcttataac gacaatatgg ccgctggcgc actgacagca ttaaaagata atggcattgc
    61441 gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc gttacaccga
    61501 cccgcaatta acgaccgtgc gttatcccat tgcttcaatg actaaattag ccaccgaact
    61561 ggccttgcag ggggcagcag gcaatattga ttctcgtgcc agccactgtt ttatgccgac
    61621 gttagtgcgt cgtcattctg tcgcaacgcg ccagaatgcg gcggcgatca ctaactcaac
    61681 aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct taacaatgtg
    61741 atagctatga ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa taatcacttt
    61801 caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt taccgggcat
    61861 ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt aactctactg
    61921 ccccgccgag catttatctc aagcactacc ctgcataaga aaaaccggag ataccatgaa
    61981 taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg ccgctgcaca
    62041 cgctgctgat actcgcattg gtgtaacaat ctataagtac gacgataact ttatgtctgt
    62101 agtgcgcaaa gctattgagc aagatgcgaa agccgcgcca gatgttcagc tgctgatgaa
    62161 tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc tggcgaaagg
    62221 ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg tgattgagaa
    62281 agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc gtaaggcgct
    62341 ggatagctac gacaaagcct actacgttgg cactgattcc aaagagtccg gcattattca
    62401 gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga acaaagacgg
    62461 tcagattcag ttcgtactac tgaaaggtga gccgggccat ccggatgcag aagcacgtac
    62521 cacttacgtg attaaagagc tgaacgataa aggcatcaaa actgaacagt tacagttaga
    62581 taccgcaatg tgggacaccg ctcaggcgaa agataagatg gacgcctggc tgtctggccc
    62641 gaacgccaac aaaatcgaag tggttattgc taacaacgat gcgatggcaa tgggcgcggt
    62701 tgaagcgctg aaagcacaca acaagtccag cattccggtg tttggcgtcg atgcgctgcc
    62761 agaagctctg gcgctggtga aatccggtgc actggcgggc accgtactga acgatgctaa
    62821 caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca aaggtgcggc
    62881 tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg ttggcgtaga
    62941 taaagacaac ctggctgaat tcagcaagaa ataagactga ttgggcgcag tcgtttactg
    63001 cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga ctccgtcttc
    63061 cggggaatac ttgttggaaa tgagcggtat caacaagtcc tttcctggtg ttaaggcact
    63121 tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg gggaaaacgg
    63181 tgcaggaaaa tcgacattat taaaatgcct gtttggtatt tatcaaaaag actccggcac
    63241 cattttattc cagggtaaag agatcgattt ccattccgcc aaagaggcac tggaaaatgg
    63301 tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga tggataacat
    63361 gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa tgtaccgcga
    63421 aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc gcgtcggcac
    63481 attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct ataacgcgaa
    63541 aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca atcatctgtt
    63601 cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct cgcataaaat
    63661 ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc agtggatcgc
    63721 caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg ttgggcgttc
    63781 tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc tcgaggtgcg
    63841 taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc tgcataaagg
    63901 ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg ttgagacgtt
    63961 atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac agatcaataa
    64021 ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg agcgccgctc
    64081 aacgggaatt tatgcctatc tggatattgg ttttaactcg ttaatttcca atattcgcaa
    64141 ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata cccagtgggt
    64201 gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt cgctctccgg
    64261 tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag aaatattaat
    64321 gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt atcagttaat
    64381 tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa tgcctgagtt
    64441 gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg gaattgtcga
    64501 tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc tttaagatta
    64561 ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga aagagggcgg
    64621 tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc caacattttt
    64681 aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta ttatcgcgct
    64741 cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc gtcaggtagg
    64801 gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca acaaagtgtt
    64861 cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg ccattggtgc
    64921 ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc cgttcattac
    64981 cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg actttgtcgg
    65041 ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg gctttgtcgc
    65101 gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg tggcgttcgt
    65161 ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg gcggtaaccc
    65221 ggaagcggca aaagtatctg gtgtcaacgt cggcctgaac ctgctgatga tctacgcgtt
    65281 gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg gctctgccac
    65341 caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg taggcggtgt
    65401 atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa ttatttttac
    65461 cgtcatcaac tatggcctga cgtatatcgg cgttaaccca tactggcagt acatcatcaa
    65521 aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc gtaagaagtg
    65581 atgtgattgt ggatattgcc ggatgcgctg catccggcaa tcggtgccaa atgccggatg
    65641 cgtctctggt tttacaacgt taccgggtgt tctttctcgc ctttcttaaa cttcacttcc
    65701 ccgagctcaa tacaacccac cgggcagacg tgaccacaaa gcagacaacc cacacatttc
    65761 tcggtattac aatgcggtgt gcgggttttc tcgctccatt ccatcgcctg gtgaccgccg
    65821 tcgtaacagg aaatataaca gcgtccacag ccaacacatt tatcaagatt gatacgggga
    65881 tagacaatat aactgcggtc taaatcttcc gccgggacaa tattgttatt cgccagacct
    65941 accatctcct gcagcgaatc aaatccctga tcggcgagat aatgcgacaa cccgcttgcc
    66001 atatcttcca ctatccgata cccgtactgc atgatgccgg tggtcacctg taacgttgct
    66061 gcgccgagca ataaaaactc agccgcatcc tcccaggttt caatgccgcc gataccgctg
    66121 attgggaaat cgcgcagttc tggatgggtg cgcatctgct gaatgaagcg cagggcgatc
    66181 ggttttaccg ctttaccgga atatccggaa atactcgatt ttccgttaac gatcggcata
    66241 ccgattttct gattaagatc gatattggtg atggatttaa cggtgttaat cgccgcaatg
    66301 ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc acatatcacc gatattcggc
    66361 gtcatcttcg ccagcattgg cagcgtggaa ccccgtttca ctgcccgaca atatttttct
    66421 accagctccg ggctttgccc gacatcgcta cccatcgcat gagaagtcat ttgcggacag
    66481 gagaagttac actcgatcat atccgcgcca gcttcttgca ccaggcgcgc cagctcctcc
    66541 cattgctgct cattttcccc catgatcgaa gcgatcaata ctttgtccgg gtaatcttcc
    66601 ttcagccgac gcagggcggc cagattctct tccaacggat gttcagcaat ctgctccata
    66661 tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg cggcgagact
    66721 tcgttggcga taaaaaagcc gatcgtttta aacaccacac cgccccaacc tgtgtcgtag
    66781 gctttggcac acatctcata gcagttgcct accggcgaag aagagaggca gaacgggttg
    66841 ggaaacttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag caagctccct
    66901 ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca tagaccacgg
    66961 ttttgtcacc ctcaacaata tcgcccgcag caaagacttg cggatcgcgg gtctgataat
    67021 tttgtgtatt gatagtgtta cgctgcggct ctaactccgc aaaggcatcc agtctggcat
    67081 gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgccc ggtaaccgta
    67141 catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca tcgatgatcg
    67201 aaacgcccag ctcccgggcg ctggcaaact ctttttcgct tgccggaaac tcatctaact
    67261 cttcacgcgc tacgcaggtt accgcctgac agccgagaac tttcagcgtg ctggctacgt
    67321 ccatcgcaac atcaccgccg ccgataatta atgcgctttg tggaatgctg atatcgcctt
    67381 gcgcctgtcg tgcacgttgc aggaagtcca cggcaatctc aacgtcgcta tgctcaaaca
    67441 gcaataaccc ggaaccgctc gataacccca cggtgaccag taccgcgcgg ttttctgctt
    67501 tcagctgctc aagggtgagt gtgttaccga cttcgttgtt gcacttaatg gtcacgccca
    67561 ttttttcaat acgggcgatc tctgcatcca gcactgattg cggtaaccgg aattgcggaa
    67621 taccgttacg caaccagcca ccggggtgcg cttctttctc ataaatcgtg acgtcataac
    67681 cctggtttgt cagtgttaca ctggcctgca atccggcagg acctgcgcca ataatcgcga
    67741 ctttgccgag cgttttagta ccgggctgat aaatttccat cccggtttgt tgttcaaaat
    67801 cagtaacaaa acgctgtaag cggccgatat caatgggagc atcaacaccg gcacgggtac
    67861 aaccgctttg acataatttc tccgtcgggc acactctggc acaaacggca ccgagggcat
    67921 tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt gagcgaataa
    67981 atttccccgg atcggtctgt gccgggcaag cctgactaca gggagcgtca tgacataata
    68041 aacagcgaga ggcttcttta atcgccagta aaggcgtaaa agccggagtg agttcatcca
    68101 gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattaag gcgaacgcta
    68161 aatatactcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca ccccagtcac
    68221 ttacttatgt aagctcctgg ggactcactc acttgtcgcc ttcctgcaac tcgaattatt
    68281 tagagtataa atattgcgtg ctgatattaa ggctatgcgc gtggggaaat ttatactgtg
    68341 aaaggattca caaatagctg cggttcagaa tgttatatcc gcagcttatt gagtgaagta
    68401 ataattattc tcaataatta acagaaaaga gaaaatatta acgatagaaa ataagataaa
    68461 caatcatgcc gatagataaa attccgaagc cgatatatgc caccatataa gcgaaaaaga
    68521 gtccgggatt ttttgctcgc cagacataca ccggttccgg tgcattttta acccgtttat
    68581 caatcgcgcc tgcgcgccag cctttccatg cttcgcggaa acaaaatatc gggataagga
    68641 aaaaaacggc aacgacaaaa aactgctgta catccataac tactttcctt gtttcttttg
    68701 taattcaagt aactgttcgg gtgtgacggc gggatacccc tgcgcatctt ccggcacctg
    68761 gtgcatagcc ggaataggga ccagcggccc taaaaaacgc ggttcacgtt taaaaatata
    68821 aaggtcagcc agcgctccga aacgggcggc aaattcgcga atacgtactg acagcatatc
    68881 tttcggtgac ggtacggcat aacactgagc ctgaatcccc atatgcagcg caataaataa
    68941 tgcccgctca cagtggaaac gttgggtgat aataatgaaa tcattagtat cgaaaacttt
    69001 gcgtgtacgc actatggaat ccagcgtacg aaagcctgcg taatcgagaa caatatctga
    69061 tgggtcgaca ccagcagcga ttaaatcttt gcgcatggtc atcggctcat tataactttg
    69121 caatgcgtta tcgccgctca gtaatagata atttacctta ccgctgttat aggcattaat
    69181 cgctccttga atgcggtagc gataatactg attaattacg ccagtacgat aatattttgc
    69241 tgttccgagc accacaccga cctggcggta ggggagatcc tgcaattcgt cgtagatata
    69301 aggcgcggtt ttccagctca tccagcgatc gaggccgagc acagtcaaca gcagcaagcc
    69361 gatcaggact aacaggctga ggaacacgcg ctttaacatg caggagactc gatctaaggg
    69421 aaatttccat caggctactt tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc
    69481 gggattttcg acaccaggag agggctggca agccggacaa ggcgttcacg ccgcatccgg
    69541 caccaggctt aagcgagaag cacgcggtcg atactgtgac agccgagcgc tttcagcgtt
    69601 gcggaggtgg catcccactg aatcaacggc gcatcggctt tttctgccag aaccgcgcgc
    69661 tggatatccg ggtaatcata acccttgaga ttcaacagaa ttaacgctcc ctgcaacggt
    69721 ggcagagtcg ggttgaatgc ggcgttttca gcgtagctgc cactgaaaat acgaccgtct
    69781 ttacattcca gcgcgacacc gcttggcgac ttactgtaag gcatgtgcga acggtttgcc
    69841 gctgcaatcg ccgcctgaga aagcgcatca ccagtcagcg catagccgtg atcctgctcg
    69901 tccatcagca gcgttttaat ctccagatct ttcggcccaa aggcatccgg cagatagtca
    69961 cgcagcgcgt gtgcctcgcg gcccggcaga tgaatacgca gatccagacc gctgttcagt
    70021 tcattcataa actgacggca gtgaccacaa ggcgtgtagt taacggtgat ggctgcaagc
    70081 gcttcttcac cactcaacca ggcgtggctg atcgcgcttt gttcggcatg aacggtttgc
    70141 tgcattgtcg caccaataaa ttccatattg gcaccgaaat accaggttcc gctcacaccg
    70201 cgcgcaattg cgccaacatt aaaattcgac aatggcgtac gcgcacaggc cgccgccagc
    70261 ggaagtagtg cgaatgccag cgcgtcttcg tccagccccg ttgcgctctt cagcgatgag
    70321 acttgctccc cggtcaacaa agcggggaag tacttgtctg ccagaatagg ttccagtgca
    70381 gattgcaagt tatccgcaag ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg
    70441 cctcataatc atgtaatggg ataacagttt acgaacccgt tatggcttta tatgtgatct
    70501 gaatctcatt aattatgcaa cgcgtgaaac ttacatcaaa aatgcgcgac gcatcgcaaa
    70561 ttttagccca ttactgccag aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt
    70621 atcccgcata acaccagcgc tagcgaacta aatgcacctt cctgataatc cagctcggcg
    70681 cagcgcgccg taccgagggc gtgcgaggca gtccccattg ccagaccgcg cgcagctttg
    70741 gtacgaatac gcatcgcatt aagcaatgta tggccaaata ccgcgccgag gatgccgacg
    70801 aaaatcacgc aaactgcgct gattgccgga ataccgccaa tactgccgcc aaccgccatt
    70861 gcaataggcg tggtgactga tttcggcagg attgacgcgg cgatttccgg tgaagcgccc
    70921 atcaacaatg ccacggaagt ccccgtcacc atcgcaacca cgctgccgat aaaacagatg
    70981 gtgatgatcg atttccagcg cgcgcggatc tggtgtagct gctcatataa aggataggcc
    71041 agcgcgacga ccgccggttg cagcagatcg tttagcactt cgctaccttt aaagtagctg
    71101 tcgtaagaga tgccggtcag cattaaaaaa ggaatgatca ccaccattgc taccagcaac
    71161 gggttaagca ggggaaactt ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc
    71221 aacgtcagcg gtaatgacca ccagatattc gccatcattc ttctgatcct ttctgaccta
    71281 cgactttacg ttcaccgtgt accagttgtg aactccagct caccaccaga aaaaccacca
    71341 gcgtactgac agcacaggaa accactaccg ggccaaactg cgcgcggagc aaatcaaaat
    71401 attgcatgac gcctacgcca atcggcacaa acaatagcgc catatagcga atcagtacgt
    71461 agcaccccgg attgacccat ttcgccggaa gaatttgcaa ggccagcagg acaaacagga
    71521 tcagcatccc gatgatgctg cccggaatgg ttaccggtag cagggaagca atgaaaatgc
    71581 ctgcatacag gcaggcataa atcaggacga aagcgcgtaa atattgccag ataatgttca
    71641 gtgtcttact cataatcaac accccagatg agatggattc atcatacaat taattcgtaa
    71701 aacgtgctac cgatcacatt aagaaatcag cgcttatttt ttcgctcccc ggcattacgc
    71761 aatgcgcaac gaactcaggc atacatcaag catagagagg gattgcagtt tgcagcaatc
    71821 cctctttgta gacctttaat ccgctgacca taggcgctaa catagcgcct cattattttg
    71881 cgggtgatga tatgcgtgtg ttactggcac cgatggaggg agtgcttgac tctctggtgc
    71941 gtgaattgct gaccgaagtt aacgactacg atctgtgcat caccgagttt gtccgcgtgg
    72001 tggatcaact gctgccggta aaagtctttc atcgcatttg ccctgagcta caaaacgcca
    72061 gccggacacc atctggtacg ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc
    72121 tggcagagaa cgccgcccgt gcggtggagt taggttcctg gggcgtggat ctcaattgcg
    72181 gctgcccgtc gaaaacggtt aacggtagcg gcggcggcgc gacgttactc aaagatcctg
    72241 aactcatcta ccagggtgca aaagcgatgc gtgaagctgt accggcgcat ttgcccgtca
    72301 gcgtgaaagt gcgtctgggc tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg
    72361 ttcaacaggc tggcgctacg gagctggtgg tgcatgggcg gacgaaagag cagggttacc
    72421 gcgcggagca tattgactgg caggcgattg gcgatattcg ccagcggctg aatattccgg
    72481 tgattgccaa cggtgaaatc tgggactggc agagcgcgca acaatgcatg gcgatcagcg
    72541 gctgcgacgc agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg agccgggtag
    72601 taaaatataa cgaaccgcga atgccgtggc cggaggtggt tgctttgctg caaaaatata
    72661 cccgtctgga aaagcagggc gataccgggt tatatcacgt tgcgcggatt aaacagtggt
    72721 tgagttattt gcgtaaagaa tacgatgaag caacgggatt atttcagcat gttcgggtgt
    72781 tgaataattc ccctgatatt gcaagggcta ttcaggcaat tgatatcgat aagctgcgat
    72841 aatagtggaa tcgcagctta tcagagacaa tgaatcgctt attcagcttc atcatcttgt
    72901 tgcgctcctt tcatga
//
LOCUS       DAZGCS010000008        72407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGCS010000008 DAZGCS010000000
VERSION     DAZGCS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.008"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..712
                     /gene="yadN"
                     /locus_tag="ACHK01_000566"
     CDS             128..712
                     /gene="yadN"
                     /locus_tag="ACHK01_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8095565.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            817..1557
                     /gene="yadV"
                     /locus_tag="ACHK01_000567"
     CDS             817..1557
                     /gene="yadV"
                     /locus_tag="ACHK01_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8095566.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1592..4192
                     /gene="htrE"
                     /locus_tag="ACHK01_000568"
     CDS             1592..4192
                     /gene="htrE"
                     /locus_tag="ACHK01_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO8095567.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAEPVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4209..4778
                     /gene="yadM"
                     /locus_tag="ACHK01_000569"
     CDS             4209..4778
                     /gene="yadM"
                     /locus_tag="ACHK01_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8095568.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4793..5395
                     /gene="yadL"
                     /locus_tag="ACHK01_000570"
     CDS             4793..5395
                     /gene="yadL"
                     /locus_tag="ACHK01_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO8095569.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5422..6018
                     /locus_tag="ACHK01_000571"
     CDS             5422..6018
                     /locus_tag="ACHK01_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8095570.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6002..7345
                     /locus_tag="ACHK01_000572"
     CDS             6002..7345
                     /locus_tag="ACHK01_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO8095571.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7458..8252
                     /gene="panB"
                     /locus_tag="ACHK01_000573"
     CDS             7458..8252
                     /gene="panB"
                     /locus_tag="ACHK01_000573"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO8095572.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8264..9115
                     /gene="panC"
                     /locus_tag="ACHK01_000574"
     CDS             8264..9115
                     /gene="panC"
                     /locus_tag="ACHK01_000574"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO8095573.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9189..10091)
                     /gene="rpnC"
                     /locus_tag="ACHK01_000575"
     CDS             complement(9189..10091)
                     /gene="rpnC"
                     /locus_tag="ACHK01_000575"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO8095574.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            10365..10745
                     /gene="panD"
                     /locus_tag="ACHK01_000576"
     CDS             10365..10745
                     /gene="panD"
                     /locus_tag="ACHK01_000576"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO8095575.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10749..11978)
                     /gene="yadE"
                     /locus_tag="ACHK01_000577"
     CDS             complement(10749..11978)
                     /gene="yadE"
                     /locus_tag="ACHK01_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO8095576.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12042..12482)
                     /gene="yadI"
                     /locus_tag="ACHK01_000578"
     CDS             complement(12042..12482)
                     /gene="yadI"
                     /locus_tag="ACHK01_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8095577.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12587..13357)
                     /gene="yadH"
                     /locus_tag="ACHK01_000579"
     CDS             complement(12587..13357)
                     /gene="yadH"
                     /locus_tag="ACHK01_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8095578.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13354..14280)
                     /gene="yadG"
                     /locus_tag="ACHK01_000580"
     CDS             complement(13354..14280)
                     /gene="yadG"
                     /locus_tag="ACHK01_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8095579.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14389..15051
                     /gene="can"
                     /locus_tag="ACHK01_000581"
     CDS             14389..15051
                     /gene="can"
                     /locus_tag="ACHK01_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO8095580.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15092..15628)
                     /gene="hpt"
                     /locus_tag="ACHK01_000582"
     CDS             complement(15092..15628)
                     /gene="hpt"
                     /locus_tag="ACHK01_000582"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO8095581.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15834..18224
                     /gene="gcd"
                     /locus_tag="ACHK01_000583"
     CDS             15834..18224
                     /gene="gcd"
                     /locus_tag="ACHK01_000583"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO8095582.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18271..19821)
                     /gene="cueO"
                     /locus_tag="ACHK01_000584"
     CDS             complement(18271..19821)
                     /gene="cueO"
                     /locus_tag="ACHK01_000584"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO8095583.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19987..20334
                     /gene="yacC"
                     /locus_tag="ACHK01_000585"
     CDS             19987..20334
                     /gene="yacC"
                     /locus_tag="ACHK01_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO8095584.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20440..21306
                     /gene="speE"
                     /locus_tag="ACHK01_000586"
     CDS             20440..21306
                     /gene="speE"
                     /locus_tag="ACHK01_000586"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO8095585.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21322..22116
                     /gene="speD"
                     /locus_tag="ACHK01_000587"
     CDS             21322..22116
                     /gene="speD"
                     /locus_tag="ACHK01_000587"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO8095586.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22154..22516)
                     /gene="yacL"
                     /locus_tag="ACHK01_000588"
     CDS             complement(22154..22516)
                     /gene="yacL"
                     /locus_tag="ACHK01_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO8095587.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVIVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22692..25289)
                     /gene="acnB"
                     /locus_tag="ACHK01_000589"
     CDS             complement(22692..25289)
                     /gene="acnB"
                     /locus_tag="ACHK01_000589"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO8095588.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAVVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25644..27401
                     /gene="yacH"
                     /locus_tag="ACHK01_000590"
     CDS             25644..27401
                     /gene="yacH"
                     /locus_tag="ACHK01_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO8095589.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27472..28896)
                     /gene="lpdA"
                     /locus_tag="ACHK01_000591"
     CDS             complement(27472..28896)
                     /gene="lpdA"
                     /locus_tag="ACHK01_000591"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO8095590.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29221..31113)
                     /gene="aceF"
                     /locus_tag="ACHK01_000592"
     CDS             complement(29221..31113)
                     /gene="aceF"
                     /locus_tag="ACHK01_000592"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO8095591.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKIAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31128..33791)
                     /gene="aceE"
                     /locus_tag="ACHK01_000593"
     CDS             complement(31128..33791)
                     /gene="aceE"
                     /locus_tag="ACHK01_000593"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO8095592.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33952..34716)
                     /gene="pdhR"
                     /locus_tag="ACHK01_000594"
     CDS             complement(33952..34716)
                     /gene="pdhR"
                     /locus_tag="ACHK01_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO8095593.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35257..36630
                     /gene="aroP"
                     /locus_tag="ACHK01_000595"
     CDS             35257..36630
                     /gene="aroP"
                     /locus_tag="ACHK01_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO8095594.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36673..37527)
                     /gene="ampE"
                     /locus_tag="ACHK01_000596"
     CDS             complement(36673..37527)
                     /gene="ampE"
                     /locus_tag="ACHK01_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO8095595.1"
                     /translation="MTLFTTLLMLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37524..38075)
                     /gene="ampD"
                     /locus_tag="ACHK01_000597"
     CDS             complement(37524..38075)
                     /gene="ampD"
                     /locus_tag="ACHK01_000597"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO8095596.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38163..39056
                     /gene="nadC"
                     /locus_tag="ACHK01_000598"
     CDS             38163..39056
                     /gene="nadC"
                     /locus_tag="ACHK01_000598"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO8095597.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39259..39699
                     /gene="ppdD"
                     /locus_tag="ACHK01_000599"
     CDS             39259..39699
                     /gene="ppdD"
                     /locus_tag="ACHK01_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO8095598.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39709..41094
                     /gene="gspE"
                     /locus_tag="ACHK01_000600"
     CDS             39709..41094
                     /gene="gspE"
                     /locus_tag="ACHK01_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO8095599.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41084..42286
                     /gene="hofC"
                     /locus_tag="ACHK01_000601"
     CDS             41084..42286
                     /gene="hofC"
                     /locus_tag="ACHK01_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO8095600.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42321..43364)
                     /gene="guaC"
                     /locus_tag="ACHK01_000602"
     CDS             complement(42321..43364)
                     /gene="guaC"
                     /locus_tag="ACHK01_000602"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO8095601.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43520..43564)
                     /locus_tag="ACHK01_000603"
     CDS             complement(43520..43564)
                     /locus_tag="ACHK01_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095602.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43589..44209
                     /gene="coaE"
                     /locus_tag="ACHK01_000604"
     CDS             43589..44209
                     /gene="coaE"
                     /locus_tag="ACHK01_000604"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO8095603.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44209..44952
                     /gene="zapD"
                     /locus_tag="ACHK01_000605"
     CDS             44209..44952
                     /gene="zapD"
                     /locus_tag="ACHK01_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO8095604.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            44962..45159
                     /gene="yacG"
                     /locus_tag="ACHK01_000606"
     CDS             44962..45159
                     /gene="yacG"
                     /locus_tag="ACHK01_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO8095605.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45259..45648)
                     /gene="mutT"
                     /locus_tag="ACHK01_000607"
     CDS             complement(45259..45648)
                     /gene="mutT"
                     /locus_tag="ACHK01_000607"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO8095606.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(45708..48413)
                     /gene="secA"
                     /locus_tag="ACHK01_000608"
     CDS             complement(45708..48413)
                     /gene="secA"
                     /locus_tag="ACHK01_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO8095607.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48475..48987)
                     /gene="secM"
                     /locus_tag="ACHK01_000609"
     CDS             complement(48475..48987)
                     /gene="secM"
                     /locus_tag="ACHK01_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO8095608.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49218..50135)
                     /gene="lpxC"
                     /locus_tag="ACHK01_000610"
     CDS             complement(49218..50135)
                     /gene="lpxC"
                     /locus_tag="ACHK01_000610"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO8095609.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50236..51387)
                     /gene="ftsZ"
                     /locus_tag="ACHK01_000611"
     CDS             complement(50236..51387)
                     /gene="ftsZ"
                     /locus_tag="ACHK01_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO8095610.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51448..52710)
                     /gene="ftsA"
                     /locus_tag="ACHK01_000612"
     CDS             complement(51448..52710)
                     /gene="ftsA"
                     /locus_tag="ACHK01_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO8095611.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52707..53537)
                     /gene="ftsQ"
                     /locus_tag="ACHK01_000613"
     CDS             complement(52707..53537)
                     /gene="ftsQ"
                     /locus_tag="ACHK01_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO8095612.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53539..54459)
                     /gene="ddlB"
                     /locus_tag="ACHK01_000614"
     CDS             complement(53539..54459)
                     /gene="ddlB"
                     /locus_tag="ACHK01_000614"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8095613.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54452..55927)
                     /gene="murC"
                     /locus_tag="ACHK01_000615"
     CDS             complement(54452..55927)
                     /gene="murC"
                     /locus_tag="ACHK01_000615"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO8095614.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(55981..57048)
                     /gene="murG"
                     /locus_tag="ACHK01_000616"
     CDS             complement(55981..57048)
                     /gene="murG"
                     /locus_tag="ACHK01_000616"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO8095615.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57045..58289)
                     /gene="ftsW"
                     /locus_tag="ACHK01_000617"
     CDS             complement(57045..58289)
                     /gene="ftsW"
                     /locus_tag="ACHK01_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO8095616.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58289..59605)
                     /gene="murD"
                     /locus_tag="ACHK01_000618"
     CDS             complement(58289..59605)
                     /gene="murD"
                     /locus_tag="ACHK01_000618"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO8095617.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59608..60690)
                     /gene="mraY"
                     /locus_tag="ACHK01_000619"
     CDS             complement(59608..60690)
                     /gene="mraY"
                     /locus_tag="ACHK01_000619"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO8095618.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60684..62042)
                     /gene="murF"
                     /locus_tag="ACHK01_000620"
     CDS             complement(60684..62042)
                     /gene="murF"
                     /locus_tag="ACHK01_000620"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO8095619.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62039..63526)
                     /gene="murE"
                     /locus_tag="ACHK01_000621"
     CDS             complement(62039..63526)
                     /gene="murE"
                     /locus_tag="ACHK01_000621"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO8095620.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63513..65279)
                     /gene="ftsI"
                     /locus_tag="ACHK01_000622"
     CDS             complement(63513..65279)
                     /gene="ftsI"
                     /locus_tag="ACHK01_000622"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO8095621.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65295..65660)
                     /gene="ftsL"
                     /locus_tag="ACHK01_000623"
     CDS             complement(65295..65660)
                     /gene="ftsL"
                     /locus_tag="ACHK01_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO8095622.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65657..66598)
                     /gene="rsmH"
                     /locus_tag="ACHK01_000624"
     CDS             complement(65657..66598)
                     /gene="rsmH"
                     /locus_tag="ACHK01_000624"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO8095623.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66600..67058)
                     /gene="mraZ"
                     /locus_tag="ACHK01_000625"
     CDS             complement(66600..67058)
                     /gene="mraZ"
                     /locus_tag="ACHK01_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO8095624.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67660..68664)
                     /gene="cra"
                     /locus_tag="ACHK01_000626"
     CDS             complement(67660..68664)
                     /gene="cra"
                     /locus_tag="ACHK01_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO8095625.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68746..68832)
                     /locus_tag="ACHK01_000627"
     CDS             complement(68746..68832)
                     /locus_tag="ACHK01_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO8095626.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(68844..69335)
                     /gene="ilvN"
                     /locus_tag="ACHK01_000628"
     CDS             complement(68844..69335)
                     /gene="ilvN"
                     /locus_tag="ACHK01_000628"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8095627.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69338..71062)
                     /gene="ilvI"
                     /locus_tag="ACHK01_000629"
     CDS             complement(69338..71062)
                     /gene="ilvI"
                     /locus_tag="ACHK01_000629"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO8095628.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71181..71225
                     /locus_tag="ACHK01_000630"
     CDS             71181..71225
                     /locus_tag="ACHK01_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095629.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(71380..72324)
                     /gene="leuO"
                     /locus_tag="ACHK01_000631"
     CDS             complement(71380..72324)
                     /gene="leuO"
                     /locus_tag="ACHK01_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO8095630.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17792 a        19490 c        17556 g        17569 t
ORIGIN      
        1 ggttgtaaat tttggtaaca aatatcattg ctcattaata tctagcatct gcttataaca
       61 tttgtgtcat gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag
      121 gatttttatg tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc
      181 aggtactgct tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga
      241 taacacctgt gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac
      301 tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa
      361 acctttcagc atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa
      421 aatgaccttc ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc
      481 tattaacaac ccgtctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat
      541 caaacaggtt caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac
      601 aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc
      661 aactgctggt tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac
      721 caattaaatt gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt
      781 attccctgtt ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca
      841 acagctttat gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc
      901 atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa
      961 aataaaggga ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct
     1021 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
     1081 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
     1141 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
     1201 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
     1261 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
     1321 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
     1381 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
     1441 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
     1501 catttttacg ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca
     1561 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
     1621 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
     1681 tgctgaacct gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
     1741 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
     1801 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
     1861 aaacgcacaa gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga
     1921 tataaataac gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa
     1981 tttgacggaa attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga
     2041 tatagacgtt cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt
     2101 atgggaaaat ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga
     2161 aactcctggt cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg
     2221 tgcatggcga ctgcgtgcct caggcaacta caactggatg accgattctg gcagtaatta
     2281 tgattttaag aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct
     2341 tggtgagtct tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt
     2401 atacagcgac agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg
     2461 cgttgctaat accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac
     2521 gactgtgccg ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga
     2581 tcttattgtt actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc
     2641 atcggtcgtt caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt
     2701 cttaaaagat gatattcagg atgagcctaa tttatttcag gccagctact actacggtct
     2761 gaataactat ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg
     2821 tttgttaggt cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc
     2881 caatgttcgt ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa
     2941 caagttattc gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca
     3001 gaattacctt ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca
     3061 agatcttgaa ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacggtcag
     3121 tattaaccaa ccgctaaaat ttgagaaaaa agattacggt tcattttatc tttccggaag
     3181 ttggtccgat tactgggctt ccggacaaaa tcgtagcaat tactctattg gctacagtaa
     3241 cagtgcatcc tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga
     3301 cactgacgat agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga
     3361 acaacggact tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa
     3421 taaccaactc aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt
     3481 aaatactggc tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc
     3541 cagctatgaa tcaccgtggg gaagcctggc aggttcagtt tctgcaaata gcgataacag
     3601 tcgtcaagtg tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt
     3661 cagtaatgac agttttagcg actccgatac actggcggta gttcaggctc caggtgctca
     3721 aggagcgcga ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag
     3781 cgctctttct ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga
     3841 tgttgaatta aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc
     3901 tgattttgaa accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa
     3961 aaatattcca tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg
     4021 acagggtgga caagcatttg ttcgtggtat tgagcagcag ggaaatatca gcattaaatg
     4081 gctcgaagaa agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga
     4141 aaaaatagcg caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg
     4201 gacgtcaaat gataaaaata acgccacata aaatagcgat actgatggga atattattat
     4261 caccatcagt atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa
     4321 cctgtaacat cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat
     4381 tgagaatccc taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg
     4441 attttaaact ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga
     4501 ccggaaatgc atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt
     4561 cgacgacaag taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc
     4621 ttaaacctaa cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg
     4681 gtcttgaaat gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt
     4741 ttcgtgcact ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt
     4801 taaaaattta cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt
     4861 accctcttat gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg
     4921 tacttgtact gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg
     4981 cgatgtatat atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt
     5041 caaagactgt gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac
     5101 atgcgaaggt actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa
     5161 agcaagtgcc gtcgccgttg aagtctggag caccgaaact ccggcaacag ggagcgcaac
     5221 acaatttagt tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc
     5281 ggttgtttat tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa
     5341 tgtcactgcg ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac
     5401 tgacagatcc aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt
     5461 ctgtttctgt cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac
     5521 cttgttgcca atgtaaaaaa cagcacttgc aaaagcggaa tcagtaacca gggtaatatt
     5581 gatcttggcg tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa
     5641 ccaggtggaa aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat
     5701 gtgcttaatc agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca
     5761 aggcaaatat ttgccaatga agtcgcaacc ggggctaaaa atgtcggtgt cgttctcttt
     5821 tccattcagg acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc
     5881 gtgtatccag taatgacttc tgccctgcat aattcttcat ggaaatttta tgccagaatg
     5941 caaaaaatcg atcccgcgct ggatgtcata tctggccagg taatgagtca tatattagtt
     6001 gatgtgtact acgaataata gatcaaaaca aataactgcg actgttacag cagtagatgg
     6061 tggagtgata atgaagaaat tcttcagaca ttttcttttt ttaattctgt gcttgagttg
     6121 ttataccgcg agtgctggca ctgatgataa cgtaggatat attgttggga atagttacgg
     6181 cgttggtccg tcagatcaaa aatggagaga aacggggccg aatggcgatg ctactgtcat
     6241 attcagatat gcaacaagta caaataattt agttttctac aaaccgacgc agcttggacc
     6301 tacaggtgta aaattacagt ggagtcagtt agataccgct tctggtggtg gttttcttta
     6361 ttgcaacaga agtgatagca caagtggtag cgcaatgcgt attgaaaatg caatggttga
     6421 ctcaggtaaa atgtatggct cccataaatt atttaataca tcagttcctg gtttgtatta
     6481 cacattatta atttcaaaca tgtggtcagc ttacggtacc gtaactaacg ttagttcacc
     6541 tgggatatac attggtgact ctgcagaaca atatttttcg tggtataatc caagcgaaga
     6601 cgtgttatat tggagttgca ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg
     6661 tatttatcag acccttacaa ttgaattcta tacagataca aactttgatc caacagtaac
     6721 tcaacagatc aaattatcca gctcatcaaa ttatctgtat tcatttaaag cctatggcgc
     6781 aggccaaggt ataaatgagc atagttattt tatcaaaatc gatttcgatc tgctcaatgt
     6841 caagttgact aatcccactt gttttaccgc tatgcttagt ggaacttctg tgactggttc
     6901 tacggtaaaa atgggtgaat atagtgcaga acaaatcaga aacggtgcca caccggttcc
     6961 ttttgatatt tcacttcaaa actgcgttcg tgtgactaat attgagacaa aattagtttc
     7021 aacaaaggtt ggtactgaaa acgggcaact ccttggtaat actctcacag gtaatgacgc
     7081 agcaaaagga gtcggtgtac tcatagaagg tttagcaact agtaaaaatc ctctaatgac
     7141 attgaaacct aatgattcaa attctgttta taaagattac gacccaagag gcaaagacga
     7201 tacaacagga ggagtttacc cggatcaaga tacaggtata acataccctc tccatttcca
     7261 ggccacgcta caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag
     7321 tactttccag gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg
     7381 taattgataa atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc
     7441 atttatcagg acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg
     7501 ataaaaagcg tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg
     7561 atgaaggact taacgtcatg ctggtaggcg attcgctggg catgacggtt cagggccacg
     7621 actccaccct tcccgttacc gtcgaggata tcgcctatca taccgccgcc gtacgtcgcg
     7681 gcgcgccaaa ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac
     7741 aagcctttga aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg
     7801 gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg
     7861 gtcacttagg tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc
     7921 gcggcgatga agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg
     7981 cacagctgct ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac
     8041 tggcgatccc ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga
     8101 tgcacgacgc cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg
     8161 ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg
     8221 tttatccggg cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac
     8281 cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct
     8341 ggtgcctact atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc
     8401 ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga
     8461 agatctggca cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa
     8521 agtggattta gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca
     8581 tacttacgtt gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca
     8641 ttttcgcggc gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc
     8701 ctgcttcggt gaaaaggact ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat
     8761 gggcttcgat attgagattg tcggtgtgcc aattatgcgc gccaaagacg gtctggcgct
     8821 aagttcccgt aacggttatc tgacggcgga acaacgcaaa attgcgcctg gtctgtacaa
     8881 agttttaagt tcgattgctg acaaattgca ggctggggaa cgggatctcg atgaaattat
     8941 taccattgcg gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg
     9001 cgatgccgac acattgctgg aagtttctga aaccagcaaa cgggcagtaa ttctggtagc
     9061 cgcctggctt ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg cgtaatactt
     9121 aactggcgct acggctgatg gcgccagtta ttaatttacc ccacgtcatc tgtcataaca
     9181 caggtaactt aaataaggtt aattacctta tcaatctcag caagagataa atttgtcatc
     9241 tctgcaacgt cttcccgaga cattccttta cttagaagac gcagggcgaa ttcttgcctt
     9301 tcctcctgtc ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgcgcc
     9361 agcgtcatca tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc
     9421 gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg
     9481 ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc
     9541 tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg
     9601 tcatccggtg tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgtgcc
     9661 agctccggcg agtaaaatat atcaaaccag cacattgaga gcggataagg tgtggcctcg
     9721 ccctgataaa acagtatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc
     9781 cggtgcatgg cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg
     9841 ctttggtgtt caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag
     9901 agcacgtccg tgctgtgtcc tttcaggctc tcttcaatga aactccccga ctctaaatga
     9961 agcgtgttga ggtcacaaag ttctcgtaat tccactggca aatgtatctc cagaaagtcg
    10021 cgagccgtct ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc
    10081 ggtgcatcca tcttccctgt cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg
    10141 cgcgccgtcg tcagccgcct ctttagtgtt taccgagcag cgttcagaga aatttcaatt
    10201 cgttacaggc gatacatggc acgcttcggc gcggtacgca aactgctgac gcaaatgcca
    10261 tcagatgagt agacactcat caaaaatcgg gcaatactgc gtgagaattt cctcaaagca
    10321 ggcaccgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc
    10381 tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt
    10441 gcgccattga ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccatcgata
    10501 tctggaatgt caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt
    10561 cgagaattat ttctgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca
    10621 tcatcgccag cttcgttacc atgccagatg aagaagctcg cacctggcga cccaatgtcg
    10681 cctattttga aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg
    10741 cttgattgtt atccctgcgg ctggttactc accagccgcg acatcgtctc cagcgaatcc
    10801 gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct
    10861 ttcatggttg tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca
    10921 ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg
    10981 cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt
    11041 cggcgatagc catccacccg atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg
    11101 cgaatttcgt taagctcaga aacgctcata aattgcagcg attttgggtt ccacttctgc
    11161 gggtgacgtt tgatgcgcga ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc
    11221 aatacaggat acgcatagcg gctcaccgac ttgaggccat catcaaaggt aatcaccacc
    11281 gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagctgcgc catgctcagt
    11341 gtcgcgtatc ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg
    11401 gtcgtcgaag tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg gtgataggtt
    11461 agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc gagtcgatcg
    11521 ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg
    11581 tagcgcaaat tgtccgccag taccccaaat ggcgcactgc ccgcactcgg cgcgttatag
    11641 accggcgtat ctttccaggt aattaagttc tgattactca gcggcttgtt gaggtcaccc
    11701 aaaccgtctt caactttttg tcgcccctga actggctcga gatgcccttt atcgataaac
    11761 cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg
    11821 atttgcccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagatc
    11881 gcagcatttt cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta
    11941 aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa
    12001 caacgctttt attgtagcaa aagcgctata agaatcgtgg gttaatactc atacaaatta
    12061 tgtttgagga cgaaaggcgg atttttttgt tgttggtgcc aaagactact cacctccggc
    12121 ccacccagtt cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc
    12181 atcatctgtt caattaacgg taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg
    12241 tggcttaata atgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg
    12301 acaccctcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta
    12361 gagcttaacc cacgccagaa attcacggcc cggcactgaa gaagcgcccc atgttttttc
    12421 tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac
    12481 atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata
    12541 gccgtcaaaa ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac
    12601 cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca
    12661 gtacgccaaa ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac
    12721 cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca
    12781 aagtcagtga gtaaaagacc ccacccaaat acgtgagtgg cgttaacaca aaggttggca
    12841 ccaggctgat gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga
    12901 atagcaccgc cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg
    12961 gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc
    13021 caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt
    13081 caatattacg ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcattgg
    13141 tgatcaccga catcatgatc agccccggta cgatgaactg catatagctg aagccatgca
    13201 tatcgccaat acgcgaacct atcaggttac cgaagataat aaagtaaagg gtcatggtga
    13261 tgactggcgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc
    13321 agatgctttt tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt
    13381 aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg
    13441 aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc
    13501 aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg
    13561 acttttcggt gcgagatcga gaataaaggt ttccgatttc agcttagcca gcagcgcctt
    13621 catcgaggta ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat
    13681 ttctgcttct tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc
    13741 cttcaaaaag ccccacattg agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc
    13801 gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat
    13861 cccgccagat aacatacgcg cacgttcgtt gcgttttccc catagatcga gttgtttaag
    13921 atatttttcg ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt
    13981 taccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc
    14041 caactgacgt ttagcgttca cgacatcttt ctcgagatcg taaccaaata cgctgacccg
    14101 cccggaggtt ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc
    14161 gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag
    14221 cgcctgaacg ccgcctggat aagttttttt aagctgttga agttccagtg caatggtcat
    14281 aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg
    14341 ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata
    14401 gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg
    14461 ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac
    14521 agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt
    14581 aatgttgcta acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca
    14641 gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta
    14701 caagccgccg ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc
    14761 gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat
    14821 accttgtgtg aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg
    14881 caatcagcgt ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac
    14941 gacggcttgc tgcgtgatct ggatgttacc gccaccaacc gcgaaaccct tgagcaacgt
    15001 taccgtcacg ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat
    15061 gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga
    15121 tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat
    15181 ccgggatcga gaaaccgata aattctaccg ggacgttcac ttcacgacgg gacggtttat
    15241 ccagcagcgt acaaatcgcc agcgacttcg gttcgcgcag gcttaagatc tcacgcactt
    15301 tcgacagtgt attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga
    15361 tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt
    15421 agctggaggc ggtcataaag tcgacttcat gagatacctg aacttcacgg cacaggtccg
    15481 ccataaacat aaatgagcca cgcagcagac ccaccagcac catatcgctg ccgctgtctt
    15541 tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg
    15601 ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac
    15661 ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa
    15721 ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa
    15781 aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa
    15841 ttaacaatac aggctcgcga cgattactcg tcacgctaac agcccttttt gcagcgcttt
    15901 gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact
    15961 accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg
    16021 ccgcgctttg gctgtacgca gccctgctgc tcggcaccat gatttggggc gtctgggaag
    16081 ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc ttcggcatct
    16141 ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg
    16201 cactggtggt ggcactgctg attagcggtg gtatcctgac ctgggccgga tttaacgatc
    16261 cgcaggaaat caacggcacc ttaagcgcca atgccacacc tgcggaagct atctcacccg
    16321 ttgccgatca ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc
    16381 tgaaacaaat taacgccgat aacgtccata atctgaaaga agcctgggtg ttccgtactg
    16441 gcgatgtgaa gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag
    16501 tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca
    16561 gcggcaaaga gaaatggcat tacgatcctg agctgaaaac taacgagtct ttccagcacg
    16621 taacctgccg tggcgtctct tatcatgaag ccaaagcaga aacggcttcg ccggaagtga
    16681 tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgactg attgcgatta
    16741 acgctgaaaa cggcaaactg tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa
    16801 gcaatatgcc agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg attatcaccg
    16861 ataaaaccat cgtgatggcc ggttcagtta ccgataactt ctcaacccgc gaaacgtctg
    16921 gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt gatcccggcg
    16981 cgaaagatcc gaacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact
    17041 cctgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg atgggcgtga
    17101 ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga
    17161 ttctggcgct gaatgccact accgggaaac tggcgtggag ctaccagacc gttcaccacg
    17221 acctgtggga catggatctt ccggcacagc cgacgctggc ggacatcacc gttaatggtc
    17281 agaaagtgcc agttatttac gctccggcga aaaccggcaa catttttgtg ctcgatcgtc
    17341 gcaatggcga actggtggtt ccggccccgg aaaaaccggt gccgcaaggt gctgcgaaag
    17401 gcgattacgt tactccgacc cagccgttct ctgaactgag cttccgtccg aagaaagatt
    17461 tgagcggtgc ggatatgtgg ggagccacca tgtttgacca actggtgtgc cgcgtgatgt
    17521 tccaccagat gcgctatgaa ggcattttca cgccgccatc tgaacagggc acgctggtct
    17581 tcccgggtaa cctgggaatg tttgaatggg gcgggatttc cgttgatcca aatcgtgaag
    17641 tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtccgg
    17701 gtaacccgat ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc
    17761 cacagtacgg tgtaccgtat ggtgtcacgc tcaacccgtt cctctcaccg tttggtctgc
    17821 cgtgtaaaca gccagcatgg ggttatatct cggcgctgga tctgaaaacc aatgaagtgg
    17881 tgtggaagaa acgtattggt acgccgcaag acagtatgcc gttcccgatg cctgtaccgg
    17941 tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc
    18001 tgtttatcgc tgctacggca gataactacc tgcgcgctta caacatgagc aacggtgaaa
    18061 aactgtggca gggccgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga
    18121 atggcaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg
    18181 gcgactatat tgtggcttat gcgctgccgg atgatgtgaa ataaccataa tgcccggaga
    18241 gatccgggca tatttccgaa tacggtcttt ttataccgta aaccctaaca tcatccccgt
    18301 atcttcatgc tccagcagat ggcagtgcgc catataagca tgttctttcg gtgcgttgtg
    18361 attaaacttc accagcactt cactgacatt gccttctacc ttaacggtat ctttccagcc
    18421 cgcgcgatga gccgctggcg gtttgccatt ttctgacaag atacggaact gggtgccgtg
    18481 aatatggaac ggatgcagca tcatgtcgcc cacgccagag ataacccaac gttcgtactg
    18541 ccctttcgcc gccgcaaaca tcggcttatt catatcaaac gcctgaccgt tgattttatt
    18601 ggcatggtgg aaatcgaact tcccgccatg gttcatatga ttcatattgc cgtgccccat
    18661 atggcccatc atctggctat gatccatccc ggccatcgcc tgatcgccat atttctccat
    18721 cagcatctgc atccccatca tatcgagcat cgggtccata gaaagttgca gcttgcgtac
    18781 cgtcagccct tccagcgaag gtaacgcagg caggctactt aatgtgtctg gcaaagcacc
    18841 ggaggcacta atagcaatcg gctgaatccg cattaccgga tgcggcttgt caaacggcgc
    18901 aatcgccatc cccatctggc tgaccggtag cgtcaccagg tcaaagggtt tgttgtcgtt
    18961 aacctccacc agcacttcaa aacgctcgcc catcagcacc ggcagctcgc tcaccttcac
    19021 cggttcaggt agcagaccac cgtcgctggc aatcacatac aacgggcgat tgtcgctggt
    19081 ggcgaaattg agcgagcggg cattacagcc attgagcaaa cgcaggcgca gccaaccacg
    19141 tggggcagcg tgttgtgggt agattgcacc gttggtcagc agcgtatcgc caaaccagcc
    19201 cacggcggcg gtcatcacat ccagttgata atcaatctgc ccatcagcgt taaatttttt
    19261 atcctgaaca atcaccggaa catcatcgat accccactgt tttggcagca ttaatttcag
    19321 gatctcgtca tcttcaatta ccaccagccc agccaacccc atcgccacct gtcgcccggt
    19381 cttgccatgt tgatgtggat ggaaccagca agtagcggca ggttgatcaa cgttcaacgt
    19441 caccgagcgc ttgccacctg gcggaataat tccctgcggg ccgccgtcga cttcacccgg
    19501 tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat agatatcaac
    19561 cgttaccgct ttgccgcgct gtaatttcac cgccggcccc agcagattgc cgttatagcc
    19621 ccaggtagtt gcagttttct cgccaaaggt agactgacct gcgccaatag ttaactgaat
    19681 gcgattacgg gcatcggtcg tgagcaaatc agggatcggt aacgttgggc gttccgccgc
    19741 aaagactgcg cggctccaca gcggcaaggc tgaagccaca cccagcgcga cggaatattt
    19801 taagaaatca cgacgttgca tagttatttc cttattctta agcaggcgaa acaaaatcaa
    19861 acgttgagca tagtccttcc ccttacggga aggtcaagcc gcagacacaa tttaatcgac
    19921 aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa gcccctggta
    19981 gaagcaatga agacgttttt cagaacagtg ttattcggca gcctgatggc cgtttgcgca
    20041 aacagttacg cgctcagcga gtctgaagcc gaagatatgg ccgatttaac ggcagttttt
    20101 gtctttctga agaacgattg tggttaccag aacttaccta acgggcaaat tcgtcgcgca
    20161 ctggtctttt tcgcccagca aaaccagtgg gatctcagta attacgacac cttcgacatg
    20221 aaagccctcg gtgaagacag ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa
    20281 aagtgcaaag ccctggcccg cgattcctta agcctgcttg cctacgtcaa ataatccctg
    20341 ataccttttt gtagaaatat tgaccgtgca tccgcggtca atgttagcta ttatgttgcg
    20401 cccttttttt acgggtgtta acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg
    20461 catgaaacgc tacacgacca gtttgggcag tactttgcgg tagataacgt tctgtatcat
    20521 gaaaagaccg atcaccaaga tctgatcatt tttgagaacg ctgcatttgg tcgcgtaatg
    20581 gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt ttatctatca tgagatgatg
    20641 acccatgttc cgctactggc ccacggtcac gcgaaacatg tgctgattat cggcggcggc
    20701 gacggtgcca tgctgcgtga agtaacccga cataaaaacg ttgagtcaat cacgatggtg
    20761 gaaatcgatg cgggtgtcgt atcgttctgc cgtcagtatc tacccaacca taacgccggt
    20821 agctacgacg atccgcgctt taagttggtg atcgacgatg gcgtcaattt cgttaatcaa
    20881 accagccaga cctttgatgt cattatctcc gactgcaccg atcctatcgg tcccggcgaa
    20941 agccttttca cttcggcatt ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc
    21001 ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag aagccatcga cagccatcgc
    21061 aaactcagcc attacttcag cgacgttggc ttttatcagg cggcgattcc gacctattac
    21121 ggcggtatca tgacttttgc atgggcgaca gataacgacg ccttacgcca tctctcaacc
    21181 gaaattattc aggcgcgttt tctcgcttct ggcctgaaat gccgttatta caatccggca
    21241 atccatacgg cagcttttgc cttacctcag tatctgcaag acgcactggc ttcacagccg
    21301 tcctaaggag aagataagaa attgaaaaaa ctgaaactgc atggctttaa taatctgacc
    21361 aaaagtctga gtttttgtat ttacgatatc tgctacgcca aaactgccga agagcgcgac
    21421 ggttatattg cttatatcga tgaactctat aatgccaacc gtctgaccga aatcctgtca
    21481 gaaacctgtt ccattatcgg ggccaatatt cttaacatcg cccgccagga ttacgaacca
    21541 cagggtgcca gcgtcactat tctggtgagt gaagaaccgg tcgacccgaa acttatcgac
    21601 aaaacagaac accccggccc actgccagaa acggtcgttg cccatctcga taaaagtcat
    21661 atttgcgtac atacctaccc ggaaagtcat cctgaaggcg gtttatgtac cttccgcgcc
    21721 gatattgaag tctctacctg cggcgtgatt tctccgctga aggcgctgaa ttacctgatc
    21781 caccagcttg agtccgatat cgtaaccatt gattatcgcg tgcgcggttt tactcgcgac
    21841 attaacggta tgaaacactt tatcgaccat gagattaatt cgattcagaa ctttatgtct
    21901 gacgatatga aggcgctgta tgacatggtg gatgtaaatg tctatcagga aaatatcttc
    21961 cataccaaga tgttgcttaa agagttcgac cttaagcact acatgttcca caccaaaccg
    22021 gaagatttaa ccgacagcga gcgccaggaa attaccgctg cgctgtggaa agaaatgcgc
    22081 gagatttatt acgggcgcaa tatgccagct gtttaacgtc tcgggcggag ctcccaggct
    22141 ccgccagatt tatttacttc tgctgcacga aattacggta agccgccacg acctgcagaa
    22201 aatcctcaac gccgcatagc gacaggcttt cttcgtcgta gtagttcatc ccctcttcca
    22261 tttcatcgcc agcgaattcc agttgattgg cgcgaacaat cacctcttca ccgtccatcc
    22321 acagagtgta ctcatgccct gcccgctgcc aggaacgttc gctacctttc agtgcgtgcg
    22381 ccgcttgttc cacttcatca agcaaggcca ggttttcttt cacctcttca ttaaaccagt
    22441 gcccgaccac ttcatgcccc atggacatac gcacctttac aactccggta atatcgcgca
    22501 gaaattcgta atccatagtg tgttcctctg ctcccggcaa tgcgccgaaa tcgcattgcg
    22561 ccattgatgt aattatcgca gccttaccgg agaaaaacag cggaacggcg atggtttaga
    22621 aataaaaaaa tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt
    22681 gcgcttactt attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga
    22741 gaaagctggt tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgcc
    22801 acgtaggtct ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacaaccgcc
    22861 agttccgcag aagccaggaa gacattcgcg ccagtaccca gacggttcgg gaagttacgg
    22921 gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg
    22981 gaacagccag ggatctcgat acgcgcacca ctcttaccga agacgctgta gtagccttct
    23041 tcagtcaact gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcagt
    23101 tggcctttgt gcgcatccag cagtttaccc gcagcacgga agtgaccgat gttggtcatg
    23161 caggaaccga taaatacttc gtcgatcttc tcaccctgta ccgcagacag cggacgcgcg
    23221 tcatccgggt cgttcggtgc acagaggatt ggctctttaa tatccgccag atcgatgtcg
    23281 atcactgccg cgtattcagc atctgcatcg gcttccagca gctcaggatt cgccagccat
    23341 ttttccatgc cctgaatacg acgttccagg gtgcgacgat cgccgtaacc ttccgcgatc
    23401 atccacttca gcaggacgat gttagagttc aggtattcga tgatcggttc tttgttcagc
    23461 ttgatggtac aaccagcggc agaacgctcg gcggacgcat cggtcagttc aaatgcctgc
    23521 tcaactttca gatccggcag accttcaatt tccaggatgc ggccagagaa gatgtttttc
    23581 ttgcctttct tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcatgt
    23641 accagatcgc gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat
    23701 tccggcatat ccagcggcat tacgcctgtt gcggctgcaa atgccaccag accagaaccc
    23761 gccgggaaag agataccgat cgggaaacgg gtatgggaat caccaccggt accgacggta
    23821 tccggcagca gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac
    23881 acaccgccac ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc
    23941 ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg
    24001 cacgccaggt ctttcagttc atcacgggtc atcgggccgg tggtgtcctg ggaacctaca
    24061 gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg
    24121 cctaccattt tttgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga
    24181 cggaacacat cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc
    24241 ccgataatca gcggaatacg gccaccagca cgcacttcat caatcagcac gtcggttttc
    24301 agttcgaagg tcgccagcag ttcgccggtt tcgtggttac gcacttcacc tttgtacggg
    24361 taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt cgacttcgat tggcagtgca
    24421 cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca
    24481 ccgccgcgtt tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagtta
    24541 gtggcggatt tacgcgaaga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa
    24601 cctttctgtt gcagagcttc gatttgcttg atcggaccaa caacaccagg ctggtctggc
    24661 tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt
    24721 gaccacgcat ccggtgccgg agaaaggtca tcggtgttag tttcgccagt gactttgaag
    24781 acggtaacgg tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc
    24841 caggactgca taacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca
    24901 tagaagttat cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt
    24961 ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc
    25021 agcagttcga tggctttttc cggagtcagc agaggggatt tggcttcgcc tttcgcgata
    25081 gcagccagga agcctgcttt gacataggcg gcttcatcga cgcctggggg aacacggttg
    25141 gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca
    25201 agtgcggcca tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca
    25261 gctacgtgct tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg
    25321 cggcaggcga ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta
    25381 ccgcaacggg cagaatagca ggattttgac gatgtgttaa tctgtttaca aaaaagcaac
    25441 ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt
    25501 cgttaattcg catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta
    25561 aactaccgcc tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt
    25621 gcgatttcct tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg
    25681 cgctaatttg cagtgccggg ctttgtgccg cctctaccgg gctatatata aaaagccgca
    25741 cagtggaagc gcctgtggaa ccgcaatcga cacaacagac tgcgcctgac atcaccgcag
    25801 ttacgcttcc tgcaacggtt tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat
    25861 tcagcactgc acaaatcgat caatgggttg cgcctgtcgc gctgtacccc gattctctgc
    25921 tttcacaagt gttaatggca tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt
    25981 cgcacgataa tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt
    26041 gggacgccag cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa
    26101 acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg
    26161 actcggtaca gcgattgcgg caactggcgc aacaaaccgg ttcgctgaag tcatcaaccg
    26221 aacaaaaagt tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac
    26281 ccgtcatacc atccaatacc gttttaactg ccagccccgt cattacagag cctgcaacaa
    26341 ccgtcatttc cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa
    26401 ccgtggttta cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag
    26461 ccggagaacc gtttattgac agctttgtgc gcggattcgg ctatagcatg ggtgttgcta
    26521 ccacgtacgc actattcagc agcatcgact gggatgacga cgatcatgac catcatcatc
    26581 atgacgatga tgattatcat caccacgatg gcggtcatcg tgacggtaat ggctggcaac
    26641 acaacggcga caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc
    26701 ttactgataa gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc
    26761 atgatcagga tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagcgcca
    26821 cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc
    26881 aacgaacaca cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc
    26941 ggtttaatga agctgaaaac tatgggagct atgacgactt ccgcgacttc agccgtcgcc
    27001 aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg ttatcagtcg gcctcgcctg
    27061 agcagcgcca ggcagtccgc gagaaaatgc agactaaccc acagatccag cagcgaagag
    27121 acgcagcgcg tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg tttaaggaaa
    27181 aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag cgtgttcaat
    27241 cagcatcacc tgaacaacgt caggtttttc gggaaaaagt tcaggagagc cgcccacaac
    27301 gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca gcagtacgcg
    27361 aacgtctctc tgagcgcgga gcaaggcgac aggaaaggta aattgcagac gtaaaaaaag
    27421 cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaca attacttctt
    27481 cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg ccgccaggcc
    27541 cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca
    27601 acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg taccgacaat
    27661 cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca taccgtctgc
    27721 gcagtcggaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg tttcatagct
    27781 gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt ctggttcggt
    27841 ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg cgataacttc
    27901 agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac cgacgatatc
    27961 gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc
    28021 acggtcgtcc acttccacgc cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg
    28081 accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc
    28141 catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg tttccagcat
    28201 caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt tgtcagctgc
    28261 cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg cgtggtatac
    28321 ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt ctggtacttc
    28381 tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg gaataaacgg
    28441 cagttggatc gggcgagaac ccgctgcaat gatcgcattg tcgaagttga tcacggtttt
    28501 gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca gaccgttgac
    28561 cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct gattgatcac
    28621 tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga agacgatacc
    28681 gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca gtgctttaga
    28741 agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac
    28801 ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc
    28861 aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat atttatctcc
    28921 ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag
    28981 tcacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg cacccagaaa
    29041 tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc cgtcggtatt
    29101 ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat
    29161 aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac
    29221 ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc
    29281 accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg
    29341 cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat
    29401 agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc cgccgatgct
    29461 ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt
    29521 cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt
    29581 gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt
    29641 cttcagggtc agacgctgac cgtcttccga caacgaactg ttgaagcgag gcatctgctc
    29701 aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca catccagctt
    29761 acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg tgatatcggt
    29821 tttgtcgaag tgagtaacat gcgggatcat cacccagtta cggctcaggt tcgcaccaga
    29881 aattttctgg atacggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac
    29941 cttcggccac ggcagcatgc ctgggatacc gccgccagtc gccgccggag ctgcttctgc
    30001 acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg
    30061 gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac ggcggatcag
    30121 cggagtcgcg tgaacgtaag cgtcgttttc tgcaaattca gatttgcctt ccgctttcgc
    30181 agccggtgct gctgccggag cttcagcttt tgctgccgga gccggcgctg ccgcttcctg
    30241 tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc
    30301 agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggagc
    30361 cgggacttcc atagaagctt tatcgccttc tacggtgatc agtgactgtt cagcggcaat
    30421 tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc cgatatccgg
    30481 aacgttaact tctttcacgc cagccgctgg tgcaggggcc gctgccggag ccgcttcctg
    30541 tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca taatcagcga
    30601 gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtgc cagcaaacgg
    30661 agccggaact tccatagaag ctttatcgcc ttctacggtg atcagcgact gttcagcttc
    30721 aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc tgccgatatc
    30781 cggaacgtta acgtcttttg ccgctgcagc cgctggtgct gctgccggag ctgtttcttt
    30841 cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat
    30901 aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc
    30961 cgcctgcgga gacggaactt ccatagaggc tttgtcgcct tctacggtga tcagcgactg
    31021 ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa cttcatcagc
    31081 cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta cgccagacgc
    31141 gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac cactttctta
    31201 tcgatttcgc cacgtttagc cagttcgccc agcgccgcta caaccacgta ggaagcatca
    31261 acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc
    31321 agtacgcggt agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa cagtttcata
    31381 tagtcggtag atgccactgc cggagcgtcg ttcatcacct gagcgatata cggaacgcgc
    31441 ggagtttcca gcgggtgcag catgttccag cgttcacaat cctgaccatc tcgcgccagt
    31501 tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt cgccaggatc
    31561 tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg aactttacct
    31621 ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc ctcagcacct
    31681 tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc
    31741 tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc aacttcgtaa
    31801 gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat gtggctgtga
    31861 ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt accgccgatc
    31921 aggaagccac gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat acgctggaag
    31981 ccgaacatcg agtaatagat gtagaacgga atcatcggca gattgttggt gctgtaagag
    32041 gtcgccgctg ccagccagga acaacctgcg cccaactcgt tgatcccttc ctgcagaatc
    32101 tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc
    32161 tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc gaaagtacgc
    32221 gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt cagcattacg
    32281 ttcagggcac gaacgaaagc gatagtggta gagatctctt tgctctgctc ttccagcagc
    32341 gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg ctgacggctt
    32401 ggcagataac cgtgcagttt ctgacgctgt gcgtgcagat aggtatgctc ttcagaacct
    32461 tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg cacattgaaa
    32521 cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg cgcgatgttt
    32581 ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag gattactgtc
    32641 gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt cggatcgtga
    32701 ccaccacggt tcagtgccca gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt
    32761 tcaggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga tttgaaggtc
    32821 tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct ggtatcttta
    32881 cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca gccagcacct
    32941 tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga
    33001 cgctgcaggt tacagttgat aacgaagacc aggttatcca gtttttcacg ggtagcgatg
    33061 gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg
    33121 gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa tttagcctgg
    33181 taaatagcac caatcggccc cagacccata gatacggtcg ggaactgcca gaattccggc
    33241 atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg acggaagtta
    33301 tccagctgct cctgagtcag acgaccttcc aggaaagcac gagcgtacac gcccggggag
    33361 atgtggccct ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc acggaagaag
    33421 tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat atggccgccc
    33481 agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca gcggatagct
    33541 gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc ttcaacgggg
    33601 atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc gcctttgcgg
    33661 gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc ttcacggatg
    33721 accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa
    33781 cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg gaacctgtcc
    33841 cattgaactc tcgccggaaa gctcaataag acaggttcta cgtttagttg ccgcgctttt
    33901 atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc actaattctt
    33961 tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacgac ttctgtcgag
    34021 caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt cttccggctt
    34081 accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg gcagcatctc
    34141 gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca tcggctccat
    34201 acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg caatctgata
    34261 ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca gctctatggc
    34321 gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg cggcgtaata
    34381 agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg actcaggatg
    34441 gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc tctggacaaa
    34501 agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc
    34561 acgcaaggag ggacgggaga cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt
    34621 ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct caatcacatc
    34681 ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta agccacttgc
    34741 cgaagtcaat tggtcttacc aatttcatat ctgtgacgct aaagtaacaa agtattcacc
    34801 ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa caacttgaca
    34861 tatataacgt ttcaaagttg taactatgca caaatgtaga ctttacgtag gaaaggagtt
    34921 ttttaacgat tattaaacta taaaaatccg aattgaaccg attcacttac caattttgtg
    34981 atttttaatt caattaaaac gaatttaaat tcattctaca tattgagagg ggttgaggct
    35041 gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc
    35101 attcgctgcc gcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac
    35161 ttctttgatg taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact
    35221 gcgtagatca aaaaaacaat caccgcacga ggtttcatga tggaaggtca acagcacggc
    35281 gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg
    35341 atagggacag ggctattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt
    35401 atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa
    35461 atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc
    35521 agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg
    35581 gctgagctga ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg
    35641 gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg
    35701 tttggcgaga tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc
    35761 atcttcggcg gctggctgct attcagtggc aacggcggcc cgcaggcgac cgttagcaac
    35821 ctgtgggatc agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg
    35881 attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat
    35941 aacccggagc aaagtatacc gaaagcaact aaccaggtta tctaccgcat cctgattttc
    36001 tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat
    36061 accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg
    36121 aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc
    36181 cgtatgctgt ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat
    36241 aaacgtggtg taccagtaaa taccattctg gtgtctgcac tggtaacggc gttgtgcgta
    36301 ctgattaact accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct
    36361 gcactggtaa tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag
    36421 caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc
    36481 tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt
    36541 tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag
    36601 aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga
    36661 gggcaataat ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac
    36721 cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac
    36781 atgcggttca cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt
    36841 atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa
    36901 ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc
    36961 aatgccggac tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt attgccatgc
    37021 acgcaaaaac gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca
    37081 gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg
    37141 caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc
    37201 catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg
    37261 aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca ccagtagcgt
    37321 gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc
    37381 aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc
    37441 ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa
    37501 catcagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt
    37561 gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc
    37621 gtcatgtttt tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc
    37681 tgttgatact gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata
    37741 gaaaaatcat tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt
    37801 ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag
    37861 acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg
    37921 aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc
    37981 accagcaggg tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg
    38041 cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct
    38101 aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta
    38161 acatgccgcc tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta
    38221 atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg
    38281 atgccaacaa tgatattacg gcaaaacttt taccggaaaa ttctcgctct catgccacgg
    38341 tgatcacccg cgagaatggc gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc
    38401 aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaatg
    38461 ccaatcaatc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca
    38521 ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg
    38581 aattgctgga aggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc
    38641 gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt
    38701 ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg
    38761 tcgaaaaagc gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg
    38821 aagaacttga tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa
    38881 cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg
    38941 gcaacgtcac tgacaaaaca ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg
    39001 tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt cgctaatagt
    39061 tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac tttcttcgta
    39121 acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt
    39181 cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacca
    39241 aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg
    39301 gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg
    39361 cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag
    39421 ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa tggcattccc
    39481 tcgcctacca ccacccacta tgtttcagcc atgagtgtgg caaaaggcgt ggtgtcgctg
    39541 accgggcaag aaagtctcaa tggactaagc gtcgtcatga caccgggttg ggataacgca
    39601 aacggcgtca ccggctggac gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa
    39661 gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca
    39721 cagctcactg ccctgtgtct gcgttatcat ggagtcttgc tggatgccag cgaagaggtg
    39781 gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct
    39841 accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt
    39901 cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg
    39961 ctaactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca
    40021 gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat
    40081 gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat
    40141 attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc
    40201 gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg
    40261 ttacagcagg tgagccaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg
    40321 gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctaca
    40381 ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt
    40441 aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa
    40501 atccatccgc gtgccggact cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat
    40561 cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa
    40621 gcggcgcaaa ctggtcacct ggtgttgtct accctacaca ctaattccac ctgcgaaacg
    40681 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
    40741 gtaatagccc agcgtctggt acgcaaactt tgcccacatt gtcgccggca gcaaggggag
    40801 cccatccaca ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt
    40861 tgtgtacatt gctaccacgg tttttatggt cgtacggcct tatttgaagt tctgcccata
    40921 acgccggtca ttcgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac
    40981 gcacgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc
    41041 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact
    41101 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
    41161 gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac ttagcctgaa
    41221 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
    41281 actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga
    41341 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
    41401 gggcattgct ttttccaatg ccttattacc ctggtcagag gtatttccgc cactctatca
    41461 ggcaatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
    41521 gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc
    41581 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
    41641 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
    41701 catgacgctg gcagacttta gtggcgaatg gagctggctg ctggtgttgt tcggctttct
    41761 gctggcgata gccaataagt tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa
    41821 attgctgtta cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat
    41881 ttttacgatt ctggcgctga cacaaagtgc aggcattact tttttgcagg gcgtagagag
    41941 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
    42001 tatcagtaac ggtcatccca tctggctggc gctaaaaaat accggggagt ttagcccgct
    42061 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa
    42121 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
    42181 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta
    42241 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
    42301 tccacaccag cgttggaaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca
    42361 cggataaatg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa
    42421 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc
    42481 agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc
    42541 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
    42601 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
    42661 ccgccgccaa aggcttttgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc
    42721 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
    42781 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
    42841 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca
    42901 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
    42961 gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc
    43021 tgtttagttt tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc
    43081 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc
    43141 acagcagtca aaatatcaaa agaagccagc gcagaggcca tagaaaatgt acctacggtg
    43201 tccatatttg cggcgataat tggcacgccg gaccagctct gacctgaatg tttgaaggtg
    43261 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
    43321 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
    43381 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
    43441 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
    43501 atagtgagcg aacgtttcat caatttcgct ttcgtgcact catttttaac gttttgataa
    43561 tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat
    43621 tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
    43681 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
    43741 tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
    43801 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct
    43861 gattcagcaa gagacgcaac accagatcca gcaagctact tccccctatg tactgtgggt
    43921 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
    43981 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca
    44041 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
    44101 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
    44161 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
    44221 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
    44281 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
    44341 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
    44401 aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac
    44461 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc
    44521 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
    44581 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
    44641 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
    44701 acccaggcac ttaccatggt gctggattta attcgccagt cggctccctt ccgtaaacaa
    44761 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
    44821 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
    44881 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
    44941 gcctgttgct aaggagtgaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    45001 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    45061 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    45121 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgccgcacgt
    45181 aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    45241 gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt cattggctgg
    45301 cggaaaatca tcggcattaa gaccgactag cgacatccac tcaccgggtt gcccttcttt
    45361 accccacggc tccccttccc agctttcgac cagccagaac cacagagtta tatgcctgtc
    45421 cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacttc
    45481 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    45541 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgcgtta taaagatttc
    45601 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    45661 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    45721 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    45781 ttgcgctcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    45841 ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    45901 tcaacctctt caggcatacg tacctggact ttgctcagcg tgctgataac ttcatatttt
    45961 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    46021 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgctgccagg
    46081 tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc
    46141 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatg
    46201 cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc ggcaattggt
    46261 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    46321 tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc ttcacgaatg
    46381 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    46441 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    46501 ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca
    46561 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    46621 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    46681 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    46741 ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg ccagtcggct
    46801 ttaatttttt caatttgctc tacggtcgga ttttccagcg cggcaacttc tgcctgccag
    46861 ctaccaccga gcacaatatc tgtaccacga cccgccatat tggtcgcgat agtcaccgca
    46921 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg
    46981 acgttgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga tttttcgatg
    47041 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    47101 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    47161 attggacggt tggtcggaac aacgacggta tccagcttgt agatggagct aaattcgaaa
    47221 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    47281 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    47341 tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg accggtgtgt
    47401 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcgcg ggtaaacagc
    47461 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    47521 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    47581 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    47641 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    47701 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    47761 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    47821 cgttcttcag ggctgaacgc catattgtcg cgcaggtagt caaagccgta ttcgttgttc
    47881 gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc
    47941 aggttgatac cgacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    48001 cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt tagtgcgttc
    48061 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    48121 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    48181 acgcgcttac ttgcctcacg taccacggcg aaagcttccg ggatcagatt ttccagcact
    48241 tcgccttttt ccagacgtgc acgaaactct gcggttttcc ctttcagttc ttcgtcggag
    48301 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    48361 agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag cataataaaa
    48421 tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt gttgttaggt
    48481 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    48541 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    48601 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    48661 caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat
    48721 taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat tcgggcggcg
    48781 tgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    48841 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    48901 taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    48961 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt caaacgtatt
    49021 tagcgcacaa atcttatcat taaagcctgc agtccacagc acgaatgaag ataagttgtg
    49081 cggaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaatttaa
    49141 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    49201 cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc caacggcagt
    49261 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    49321 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    49381 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    49441 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    49501 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    49561 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    49621 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    49681 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    49741 atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    49801 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    49861 atgttatcga tgcccaagcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    49921 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    49981 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    50041 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    50101 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    50161 ccaactgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    50221 aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat ccagataatc
    50281 cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg gcttctgctc
    50341 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    50401 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    50461 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    50521 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    50581 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    50641 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    50701 cacgccagaa cccatcattg cgtagcccat ctcagacatt acggtgcgta cgtctgcaaa
    50761 gtccacgttc atcaaacccg gacgagtaat cagttcagcg ataccttgca cagcgccttt
    50821 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    50881 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    50941 cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    51001 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    51061 accggtacca ccccccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    51121 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    51181 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    51241 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc gctcgcgcac
    51301 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    51361 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagg
    51421 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    51481 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    51541 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    51601 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    51661 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    51721 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    51781 ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg ccagggtctg
    51841 acgttgcaga ctccgcggcg gacgaccacc tacgctcggc acttccacgc tctcatcttt
    51901 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    51961 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    52021 cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    52081 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    52141 cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    52201 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tccgcacgcc
    52261 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    52321 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    52381 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    52441 gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    52501 caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga ggtcgttcac
    52561 cccgccttta tccataccac gtgacgggca gcttcccacg ccaatgatat tgaccatacc
    52621 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac
    52681 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    52741 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    52801 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    52861 agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    52921 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    52981 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattggcg
    53041 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    53101 ctgaaggtgt ttccttccgc gtctaccata tgttgatcat tccaccgcgc aatcggcaca
    53161 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    53221 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcttg ggtcataaag
    53281 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    53341 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagccc
    53401 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    53461 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctatt tcgcgtgttc
    53521 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    53581 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    53641 ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt caatacgtcc
    53701 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    53761 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    53821 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    53881 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    53941 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    54001 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    54061 ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    54121 cggcgcgacc ggtaaaccgg caccttgcca cagcagtttg ctgcgtagtt tatccattga
    54181 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    54241 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    54301 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    54361 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    54421 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    54481 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    54541 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    54601 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    54661 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    54721 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    54781 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    54841 tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    54901 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgcccagtcc
    54961 cctggaagct ctccagcgca cgtaaaatag cctcgtcatc aattccctct tccgtagcaa
    55021 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc
    55081 gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    55141 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc tgacgtccca
    55201 ctcgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    55261 acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag tcgccctggt
    55321 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    55381 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    55441 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    55501 ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt cctgcaatgg
    55561 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    55621 tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatggcg ctggaaacaa
    55681 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgctccca
    55741 gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    55801 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg ccgacaaaat
    55861 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    55921 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    55981 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    56041 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    56101 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    56161 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    56221 cgccggtagt cctgccgcgg cgatttcact caccgttaaa gcaccggagc ggcaaacgac
    56281 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca ctttatgctg
    56341 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt taccgctctg
    56401 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    56461 aatgcgtgcg ccctgggaac cacccactac cagcacacga accggacctt cacgtccagc
    56521 caaacgttgc tgcggcaacg gcagcgccag tacatcggta cgcaccgggt tacccaccac
    56581 ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtggcaa tcttcgccag
    56641 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    56701 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gtaccacgtc
    56761 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    56821 cgcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa tgaaatcaat
    56881 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    56941 aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga atacatgtcc
    57001 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    57061 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    57121 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa tgtcagacct
    57181 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa gctaaaccag
    57241 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa cgctttgcgg
    57301 ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag caccacaccg
    57361 acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    57421 tactccagtt tttgtaccga gttacctaaa ccttgtcccc aaagttcgcc gcgaccaaac
    57481 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    57541 aatgcggtaa cacggcggat acggtacggt tcggcgagta tcagcaacac aaccgctgaa
    57601 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    57661 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    57721 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    57781 tcgtcgcctt tacgcaccag atagttggcg atataacaaa acagcgacag ttttgtcagc
    57841 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaaccgag
    57901 ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    57961 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat gaatgccagg
    58021 atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    58081 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcaaggcc gaaggtcagc
    58141 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    58201 acccagcctt ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    58261 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    58321 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc ggggagagca
    58381 gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt tccatagttt
    58441 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcaccgtca cgaccaaaac
    58501 agtacaaacg cacgttgtcg ccattcagat agcgcgccag tggactgaag tccgccgatt
    58561 taccatcgcc gcccagcaac aaatgcagcg tgccgtctac ctgcaagcca ttcaacgccg
    58621 cttccgtact gccgacgtta gtggctttcg aatcgttaat ccagcgtacg ccattgtgct
    58681 ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc
    58741 tggcgcgcgg caaaccagct gcgtctgcca gtgccagtgc tgccagcgca ttggtgtagt
    58801 tatgctgccc ggaaagtttc atctctttca cgttcagcac tttctcgcct ttcactcgca
    58861 gccaggtttc tccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    58921 cgcagcgttc atcagcaccg cgaatcggca ttgttaaggc atcatcagca ttgacgacac
    58981 aaactttcgc attttcgtaa atacgcaatt ttgccgcacg gtattgctgc aaaccaaacg
    59041 gatagcggtc catatgatct tcagtcacgt tcagaatggt cgccgccacc gcctgtaagc
    59101 tggaagtggt ttccagctgg aagctcgaca gttccagcac gtacagttcg cactcagcat
    59161 caagcagcat caacgctggc aggccaatat tgccacccac accaacgtta acccccgccg
    59221 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    59281 ccacaatcgg tgcttgtgct tcgcggcaga acagctcgat atcgccaacg atttcgattc
    59341 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    59401 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    59461 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    59521 cgcgagcgag gaaaaagtcc acgcaggaaa gcccagtgag gcccaggccg ataatgacga
    59581 catttttacc ctgataatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    59641 aaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    59701 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    59761 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    59821 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    59881 cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    59941 tcctgccccg actatcgccg tgcagacaat gaccagttcc ccggcgtgtc gcagatacgg
    60001 tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc
    60061 accggcgaca aatacggtcg gcataattgc caggccgtcg agaccatcgg tcaggtttac
    60121 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca accccagctg
    60181 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    60241 aaggtacagg gcgaaggcga tacccagcgc aatgaccgac atccagaaat acttccaacg
    60301 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    60361 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    60421 ccacagcagt acggagatca caatcgccgt caggatcata atcccgccca tggtcggcgt
    60481 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac caaaggaaag
    60541 tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca gcgcggtcag
    60601 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    60661 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    60721 tcttccatgg cggcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    60781 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    60841 ctggcggtgc tgatagcatg gctttgttta cccacgctta acacgcggtc aataccagca
    60901 gcttttgccg cctcgcccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    60961 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg aacagcggcg
    61021 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    61081 tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc tttgatagca
    61141 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    61201 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    61261 tccgtaccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    61321 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    61381 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    61441 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    61501 accagcgcag cttccgggcg agtcagactc acagtccagg ctatttcgcc ctgatggttc
    61561 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    61621 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    61681 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    61741 cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    61801 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    61861 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcgcc tttcagggca
    61921 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    61981 tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt tacgctaatc
    62041 atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    62101 tgccaacaat ctggtaatcc tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    62161 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    62221 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    62281 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    62341 gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc ttgcccgcac
    62401 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    62461 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    62521 gacgcgcggc ggtttttaac agatcagcca gcgggtagcc aagtgccagc agtgtcgcca
    62581 gcgcgagcag caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    62641 cgccccaact tgaactaaag cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg
    62701 ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    62761 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta acaatcgcct
    62821 gaccacaatg atgttcagaa taaagcagcc atttcgcggc ttcgtagtgt tccatatcac
    62881 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca aatttcaatg
    62941 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aacgtcgcgc
    63001 cctgatccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg gtggtatttt
    63061 ctgtcgggat cactttcccc agcagaccgt taccaacggt gcccattacc gcgctggttt
    63121 cgccaagcag ttggctccac tgcgccagca gctgggtagt cgtggttttg ccgttggtgc
    63181 ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    63241 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    63301 tttcaccgtc ggtcgcctca cctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    63361 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    63421 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    63481 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    63541 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    63601 gtacgcaata cgccgcccat gatggcacca aagaccggcg ctgaaacggc gccgccgtag
    63661 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    63721 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    63781 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    63841 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggattcc
    63901 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    63961 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    64021 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    64081 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    64141 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    64201 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    64261 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    64321 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    64381 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    64441 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    64501 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    64561 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    64621 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    64681 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    64741 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    64801 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    64861 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    64921 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    64981 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    65041 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    65101 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    65161 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    65221 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    65281 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    65341 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    65401 tcaaggatca ggttgcgcca ttcaatatct aaagcatctc gctccagcac cagttgttcg
    65461 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    65521 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    65581 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    65641 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    65701 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    65761 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    65821 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    65881 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    65941 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    66001 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    66061 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    66121 aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    66181 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    66241 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    66301 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    66361 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    66421 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    66481 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    66541 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    66601 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    66661 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    66721 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    66781 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    66841 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    66901 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    66961 tgaccggcag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    67021 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    67081 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    67141 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    67201 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    67261 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    67321 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    67381 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    67441 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    67501 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    67561 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacgcgc
    67621 cagagggaaa ttcacctggc gcgtattttt gttcgcggct tagctacggc tgagcacacc
    67681 gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac gcggttcgtc
    67741 caggcttgcc aggacaatct ccagcacacg ctctgcgaca tcgcggtgac gttgagccac
    67801 tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg tggcaattgc
    67861 caggtcagaa ggcagtttac catcgcgacg tagcgtgaca tccatcaccc cttgcaacaa
    67921 cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc atttttcgaa
    67981 taactgggca gccgcctccc gctcatagct gttggcatac aggaaatgca cttcgcgcgg
    68041 atcatctttc caggcagtac ggaaaccttg ttcacgcagg aagctgacag aaagctccgg
    68101 cagcgcgcca agataaagca ccgtctcggc gggaaactta cgtaactctt ccgccagcat
    68161 ttcggcatca tcctgatcgg caccaaccac gctggtgaaa tgttcacgat cgagggcgcg
    68221 gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag gatgctcagg
    68281 tggcaacgac gtcgaaacaa taatggcatc aacctgacgc tgtaaaaggt gctcaatgca
    68341 gcgcatttcg ttgtctggct gatcttctga gcaggcaatc agcagttgat aaccccgttg
    68401 ccgcgcctgg cgctcaagat agttagcgat gcgggtatag ctggtgttct ccagatcggg
    68461 gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg
    68521 gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt cgctcacacg
    68581 gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca ctcccgccag
    68641 ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa accatagcca
    68701 ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac tgcggcaact
    68761 gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg
    68821 caaattgcgc actgagatca ttctcaacgc attattttat cgccgcgcga aagtccgacc
    68881 acaccagagc gagcaacttc cacaattttc gccacatcgc gaatcgatgc taaaaatgca
    68941 tcaagcttat cgctggtgcc tgctaattga acggtataaa gcgagggggt gacatcgata
    69001 atttgcccac ggaatatttc cgtattacgt ttcacttcgt cacgcccgta accgctggcc
    69061 tgaattttca ccagcatgat ttcccgctca acatgcgcgc cctgccccaa ctcactcacg
    69121 cgcaagacat cgaccagctt gtgtaattgc ttttcgatct gctcaagcac tttttcatcg
    69181 cccacggtct ggatggtcat acgcgataat gtcggatcgt cggttggcgc aacggtcagg
    69241 ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat
    69301 tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc gttttgctta
    69361 accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat
    69421 cgacggtaac atcaacaaac accaggcgat tattgcacac ctgttccagc gcctcgctaa
    69481 gtttgctttc cagctcatgc ggatgagaga tctggatccc gacgtgacca taggcttccg
    69541 ccagacggac gaaatcgggt agtgattgca tataagattg tgaatgacgg ccggaataga
    69601 tcatgtcctg ccactgtttc accatcccca gatagcggtt attgagattc accaccagta
    69661 ccggcaactc gtattgcaac gcggtagaca gttcctggat gttcatctga atactgccgt
    69721 cgccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc agtgccgcag
    69781 gtaaaccaaa acccatcgtg ccgaggccac cggaattgat ccagcgacgc ggtttgtcga
    69841 atggataata aagtgcagca aacatctggt gctgcccgac atcggacgtc acgtaagcgt
    69901 ctcccttcgt caaccgccaa agagtctcga tcaccgcctg cggtttaatc ttttcactgt
    69961 gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatttgctgc caccagtcgc
    70021 ggatctcatc cagtggttga tgggcggatt cttgcgacaa gagttcaagc atttgttcga
    70081 ggacctggcg agcatccccc acaatcggga tatccgcagt cacggtttta gaaatggaag
    70141 taggatcaat atcgatatgc agaacagtgg catttgggca gtactttgcc agattgttcg
    70201 tcgttcggtc atcaaatcgt accccgacgg cgaaaatcac atccgcgtta tgcatcgtca
    70261 tattggcttc gtaggtaccg tgcattccca gcatgcccag cgcctgacga tgcgttgccg
    70321 gaaacgcccc cagccccatc aatgaggaaa caacgggcag attcaacgcc tccaccgttt
    70381 ctttcaactg ctgatggcag cccgccatga ttgccccacc gcctacgtag acaaccggtt
    70441 tttttgccgc taccagcgtt tgcagagcgc gcttaatttg ccctttatgt ccggtagtag
    70501 tgggattgta agaacgcata ctgaccgact caggccagac atagggtaat ttgttcgccg
    70561 gattaagaat atctttcggt aaatcaacga ccactggtcc tgggcgacca cttgccgcca
    70621 gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt
    70681 gtttaaccac cggtcgcgaa atccccacca tgtcgcactc ctgaaaggca tcgtaaccta
    70741 tcaacgaggt cgctacctgc ccggaaagga caactaatgg aatggaatcc atataagcgg
    70801 tggcgatgcc ggtaatcgca ttggtcgccc ctggacccga cgttaccagc acgacgccga
    70861 cttccccggt cgcgcgcgcc agaccatcgg ccatatgcac tgccgcctgc tcatgacgaa
    70921 ctaaaacatg atcgatacca cccacggtat gcaatgcatc gtaaatatca aggactgcgc
    70981 ctccgggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg acgaccatct
    71041 cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac
    71101 acattaaccg tccgatcttg cttaagcaat tggcagcaag agacataaac gcagggtaat
    71161 aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac gaaggattaa
    71221 attgataata aatcggctga atcccacaac ttacagcaat ttctctattt ttttaattaa
    71281 tcctaaaaca gagagcccac acattcagaa taaaattcta cattcaataa aaaatacaga
    71341 ataaaccaga cattcatgcc tgacctattc tgcattcagt tagcgtttgc aaattgagac
    71401 taattgctct tccatccact gatggccttt atcgcgcccg gcagcttcat gccaggagag
    71461 ataacaggtt ctgctatttt gttttaacgg cagcggtaat acctgtaatt ctaaggattc
    71521 agcgaactct tcagccagcc aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac
    71581 gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct tatctaccgt
    71641 gtcataccaa ggttgactaa atgacgcgaa acgatcgagc gaaaccgccg catgttgttc
    71701 gttataaaca tcatgtttca gtaacgggcc cttaattgtt ggatgatttt tgctggctac
    71761 cagcaccatt tcatctttaa ataatggcac gctggtaaat tcaggacgat ggaagtcttc
    71821 ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt tctgatttaa
    71881 tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat tataaatctg
    71941 cgaggtcaga atgctgtcta acgggctgca aacacaaaga tgaaatacac gttcactgct
    72001 cgcgggttca aaacctgaac caggcaattc attttgtact agttgcaatg cctgacgaac
    72061 tgagccaaaa agttgaaatg cgcgagcagt cggttgaata ccacggccat aacgaacaaa
    72121 aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg caggttgcga
    72181 catacccaga acatgagcgg cacgggtaat gttttgctcc tgcatcacgg catcgaaaac
    72241 ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg ccgtctctga
    72301 atgatctgtt tgtacctctg gcatacttaa ctccactgtc acacttaact ccctttccct
    72361 tattggaatg cagagaaata atcctgaaga atatgattta tcatgca
//
LOCUS       DAZGCS010000009        71068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGCS010000009 DAZGCS010000000
VERSION     DAZGCS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.009"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(139..3663)
                     /gene="nifJ"
                     /locus_tag="ACHK01_000632"
     CDS             complement(139..3663)
                     /gene="nifJ"
                     /locus_tag="ACHK01_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO8095631.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3937..4203
                     /gene="ydbJ"
                     /locus_tag="ACHK01_000633"
     CDS             3937..4203
                     /gene="ydbJ"
                     /locus_tag="ACHK01_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO8095632.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4200..4622)
                     /gene="hslJ"
                     /locus_tag="ACHK01_000634"
     CDS             complement(4200..4622)
                     /gene="hslJ"
                     /locus_tag="ACHK01_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO8095633.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4733..5722)
                     /gene="ldhA"
                     /locus_tag="ACHK01_000635"
     CDS             complement(4733..5722)
                     /gene="ldhA"
                     /locus_tag="ACHK01_000635"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8095634.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5930..8569
                     /gene="ydbH"
                     /locus_tag="ACHK01_000636"
     CDS             5930..8569
                     /gene="ydbH"
                     /locus_tag="ACHK01_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO8095635.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            8566..8751
                     /gene="ynbE"
                     /locus_tag="ACHK01_000637"
     CDS             8566..8751
                     /gene="ynbE"
                     /locus_tag="ACHK01_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO8095636.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            8756..9085
                     /gene="ydbL"
                     /locus_tag="ACHK01_000638"
     CDS             8756..9085
                     /gene="ydbL"
                     /locus_tag="ACHK01_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO8095637.1"
                     /translation="MMKKTLLLCAFLVGLVSSNIMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(9257..10162)
                     /gene="feaR"
                     /locus_tag="ACHK01_000639"
     CDS             complement(9257..10162)
                     /gene="feaR"
                     /locus_tag="ACHK01_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO8095638.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            10398..11897
                     /gene="feaB"
                     /locus_tag="ACHK01_000640"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             10398..11897
                     /gene="feaB"
                     /locus_tag="ACHK01_000640"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO8095639.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(11955..14228)
                     /gene="tynA"
                     /locus_tag="ACHK01_000641"
     CDS             complement(11955..14228)
                     /gene="tynA"
                     /locus_tag="ACHK01_000641"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO8095640.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPTKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(14476..16521)
                     /gene="paaZ"
                     /locus_tag="ACHK01_000642"
     CDS             complement(14476..16521)
                     /gene="paaZ"
                     /locus_tag="ACHK01_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO8095641.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQTLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            16806..17735
                     /gene="paaA"
                     /locus_tag="ACHK01_000643"
     CDS             16806..17735
                     /gene="paaA"
                     /locus_tag="ACHK01_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO8095642.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWKEGTWVREAALAHAQKQHARKVA"
     gene            17747..18034
                     /gene="paaB"
                     /locus_tag="ACHK01_000644"
     CDS             17747..18034
                     /gene="paaB"
                     /locus_tag="ACHK01_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO8095643.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            18043..18789
                     /gene="paaC"
                     /locus_tag="ACHK01_000645"
     CDS             18043..18789
                     /gene="paaC"
                     /locus_tag="ACHK01_000645"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO8095644.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            18804..19301
                     /gene="paaD"
                     /locus_tag="ACHK01_000646"
     CDS             18804..19301
                     /gene="paaD"
                     /locus_tag="ACHK01_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO8095645.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            19309..20379
                     /gene="paaE"
                     /locus_tag="ACHK01_000647"
     CDS             19309..20379
                     /gene="paaE"
                     /locus_tag="ACHK01_000647"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO8095646.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            20376..21143
                     /gene="paaF"
                     /locus_tag="ACHK01_000648"
     CDS             20376..21143
                     /gene="paaF"
                     /locus_tag="ACHK01_000648"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO8095647.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            21143..21931
                     /gene="paaG"
                     /locus_tag="ACHK01_000649"
     CDS             21143..21931
                     /gene="paaG"
                     /locus_tag="ACHK01_000649"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO8095648.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLECDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            21933..23360
                     /gene="paaH"
                     /locus_tag="ACHK01_000650"
     CDS             21933..23360
                     /gene="paaH"
                     /locus_tag="ACHK01_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO8095649.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            23350..23772
                     /gene="paaI"
                     /locus_tag="ACHK01_000651"
     CDS             23350..23772
                     /gene="paaI"
                     /locus_tag="ACHK01_000651"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO8095650.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            23772..24977
                     /gene="paaJ"
                     /locus_tag="ACHK01_000652"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             23772..24977
                     /gene="paaJ"
                     /locus_tag="ACHK01_000652"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO8095651.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            25004..26317
                     /gene="paaK"
                     /locus_tag="ACHK01_000653"
     CDS             25004..26317
                     /gene="paaK"
                     /locus_tag="ACHK01_000653"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO8095652.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            26418..27368
                     /gene="paaX"
                     /locus_tag="ACHK01_000654"
     CDS             26418..27368
                     /gene="paaX"
                     /locus_tag="ACHK01_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO8095653.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            27350..27940
                     /gene="paaY"
                     /locus_tag="ACHK01_000655"
     CDS             27350..27940
                     /gene="paaY"
                     /locus_tag="ACHK01_000655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO8095654.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHMGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            28172..29032
                     /gene="pdxI"
                     /locus_tag="ACHK01_000656"
     CDS             28172..29032
                     /gene="pdxI"
                     /locus_tag="ACHK01_000656"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO8095655.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            29096..31402
                     /gene="ydbD"
                     /locus_tag="ACHK01_000657"
     CDS             29096..31402
                     /gene="ydbD"
                     /locus_tag="ACHK01_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO8095656.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            31573..32178
                     /gene="ynbA"
                     /locus_tag="ACHK01_000658"
     CDS             31573..32178
                     /gene="ynbA"
                     /locus_tag="ACHK01_000658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8095657.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            32178..33074
                     /gene="ynbB"
                     /locus_tag="ACHK01_000659"
     CDS             32178..33074
                     /gene="ynbB"
                     /locus_tag="ACHK01_000659"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8095658.1"
                     /translation="MLEKSLATLFALLILATLINHFLVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            33090..34847
                     /gene="ynbC"
                     /locus_tag="ACHK01_000660"
     CDS             33090..34847
                     /gene="ynbC"
                     /locus_tag="ACHK01_000660"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO8095659.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            34837..36153
                     /gene="ynbD"
                     /locus_tag="ACHK01_000661"
     CDS             34837..36153
                     /gene="ynbD"
                     /locus_tag="ACHK01_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO8095660.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(36204..36809)
                     /gene="azoR"
                     /locus_tag="ACHK01_000662"
     CDS             complement(36204..36809)
                     /gene="azoR"
                     /locus_tag="ACHK01_000662"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO8095661.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            37010..40912
                     /gene="hrpA"
                     /locus_tag="ACHK01_000663"
     CDS             37010..40912
                     /gene="hrpA"
                     /locus_tag="ACHK01_000663"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO8095662.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            41184..41984
                     /gene="ydcF"
                     /locus_tag="ACHK01_000664"
     CDS             41184..41984
                     /gene="ydcF"
                     /locus_tag="ACHK01_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO8095663.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            42181..43620
                     /gene="aldA"
                     /locus_tag="ACHK01_000665"
     CDS             42181..43620
                     /gene="aldA"
                     /locus_tag="ACHK01_000665"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO8095664.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(43662..44663)
                     /gene="gap"
                     /locus_tag="ACHK01_000666"
     CDS             complement(43662..44663)
                     /gene="gap"
                     /locus_tag="ACHK01_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8095665.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            44852..45382
                     /gene="cybB"
                     /locus_tag="ACHK01_000667"
     CDS             44852..45382
                     /gene="cybB"
                     /locus_tag="ACHK01_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO8095666.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            45627..45800
                     /locus_tag="ACHK01_000668"
     CDS             45627..45800
                     /locus_tag="ACHK01_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095667.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            45826..45894
                     /gene="yncP"
                     /locus_tag="ACHK01_000669"
     CDS             45826..45894
                     /gene="yncP"
                     /locus_tag="ACHK01_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO8095668.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(45872..46021)
                     /gene="hokB"
                     /locus_tag="ACHK01_000670"
     CDS             complement(45872..46021)
                     /gene="hokB"
                     /locus_tag="ACHK01_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO8095669.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            46420..48060
                     /gene="trg"
                     /locus_tag="ACHK01_000671"
     CDS             46420..48060
                     /gene="trg"
                     /locus_tag="ACHK01_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO8095670.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(48098..49021)
                     /gene="ydcI"
                     /locus_tag="ACHK01_000672"
     CDS             complement(48098..49021)
                     /gene="ydcI"
                     /locus_tag="ACHK01_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8095671.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            49238..50581
                     /gene="ydcJ"
                     /locus_tag="ACHK01_000673"
     CDS             49238..50581
                     /gene="ydcJ"
                     /locus_tag="ACHK01_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8095672.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            50806..52461
                     /gene="opgD"
                     /locus_tag="ACHK01_000674"
                     /gene_synonym="mdoD"
     CDS             50806..52461
                     /gene="opgD"
                     /locus_tag="ACHK01_000674"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO8095673.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            52601..52825
                     /gene="ydcH"
                     /locus_tag="ACHK01_000675"
     CDS             52601..52825
                     /gene="ydcH"
                     /locus_tag="ACHK01_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO8095674.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            52888..53424
                     /gene="rimL"
                     /locus_tag="ACHK01_000676"
     CDS             52888..53424
                     /gene="rimL"
                     /locus_tag="ACHK01_000676"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO8095675.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(53419..54399)
                     /gene="ydcK"
                     /locus_tag="ACHK01_000677"
     CDS             complement(53419..54399)
                     /gene="ydcK"
                     /locus_tag="ACHK01_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO8095676.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            54523..55515
                     /gene="tehA"
                     /locus_tag="ACHK01_000678"
     CDS             54523..55515
                     /gene="tehA"
                     /locus_tag="ACHK01_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO8095677.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            55512..56105
                     /gene="tehB"
                     /locus_tag="ACHK01_000679"
     CDS             55512..56105
                     /gene="tehB"
                     /locus_tag="ACHK01_000679"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO8095678.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            56408..57076
                     /gene="ydcL"
                     /locus_tag="ACHK01_000680"
     CDS             56408..57076
                     /gene="ydcL"
                     /locus_tag="ACHK01_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO8095679.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(57175..57537)
                     /gene="tnpA"
                     /locus_tag="ACHK01_000681"
                     /pseudo
     CDS             complement(57175..57537)
                     /gene="tnpA"
                     /locus_tag="ACHK01_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            57607..58815
                     /gene="insQ"
                     /locus_tag="ACHK01_000682"
     CDS             57607..58815
                     /gene="insQ"
                     /locus_tag="ACHK01_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8095680.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTPARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(58855..60069)
                     /gene="ydcO"
                     /locus_tag="ACHK01_000683"
     CDS             complement(58855..60069)
                     /gene="ydcO"
                     /locus_tag="ACHK01_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO8095681.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIFTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            60122..60658
                     /gene="sutR"
                     /locus_tag="ACHK01_000684"
     CDS             60122..60658
                     /gene="sutR"
                     /locus_tag="ACHK01_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO8095682.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            60739..62691
                     /gene="rlhA"
                     /locus_tag="ACHK01_000685"
     CDS             60739..62691
                     /gene="rlhA"
                     /locus_tag="ACHK01_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO8095683.1"
                     /translation="MSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNASNS
                     LKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMGILE
                     LDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATIEFF
                     IHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLSMKD
                     NDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRASSGR
                     TEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDVAVT
                     EPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNRNLD
                     HNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNAEKA
                     MNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASYQRG
                     SRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPVMIT
                     KHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKNHIL
                     KMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(62783..63013)
                     /gene="yncJ"
                     /locus_tag="ACHK01_000686"
     CDS             complement(62783..63013)
                     /gene="yncJ"
                     /locus_tag="ACHK01_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO8095684.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            63235..63389
                     /locus_tag="ACHK01_000687"
                     /pseudo
     CDS             63235..63389
                     /locus_tag="ACHK01_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            63435..63851
                     /gene="hicB"
                     /locus_tag="ACHK01_000688"
     CDS             63435..63851
                     /gene="hicB"
                     /locus_tag="ACHK01_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO8095685.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            63930..65336
                     /gene="ydcR"
                     /locus_tag="ACHK01_000689"
     CDS             63930..65336
                     /gene="ydcR"
                     /locus_tag="ACHK01_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8095686.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            65581..66726
                     /gene="ydcS"
                     /locus_tag="ACHK01_000690"
     CDS             65581..66726
                     /gene="ydcS"
                     /locus_tag="ACHK01_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO8095687.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            66744..67757
                     /gene="ydcT"
                     /locus_tag="ACHK01_000691"
     CDS             66744..67757
                     /gene="ydcT"
                     /locus_tag="ACHK01_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8095688.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            67758..68699
                     /gene="ydcU"
                     /locus_tag="ACHK01_000692"
     CDS             67758..68699
                     /gene="ydcU"
                     /locus_tag="ACHK01_000692"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8095689.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            68689..69483
                     /gene="ydcV"
                     /locus_tag="ACHK01_000693"
     CDS             68689..69483
                     /gene="ydcV"
                     /locus_tag="ACHK01_000693"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8095690.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLATAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            69505..70929
                     /gene="patD"
                     /locus_tag="ACHK01_000694"
     CDS             69505..70929
                     /gene="patD"
                     /locus_tag="ACHK01_000694"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO8095691.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT        17475 a        17010 c        18761 g        17822 t
ORIGIN      
        1 gttatatgcc tttattgtca cagattttat tttctgttgg gccattgcat tgccactgat
       61 tttccaacat ataaaaagac aagcccgaac agtcgtccgg gctttttttt agaattggat
      121 aatccttatc cagagcattt aatcggtgtt gcttttttcc gcttttccgg ccatttgtgc
      181 caggaagtca tagcgttttt gcaaatcagc tgcagcatct ttccataact gttctgccac
      241 ttctggctgc tgcgaattca gccgacggaa acgttgctcg tgaagtaacg tttcttccag
      301 tgctactgac ggcgggcgtg aatccagcgc cagcggcagt ttgccttcat cggcacgacg
      361 cggatcgaag cgatatagcg gccagaagcc ggtagctgtg agttggcgca tctggtcgtg
      421 gctgagtgcc agatcgtaac catgctcttc acatgggcta taagcgatga tcagcgatgg
      481 ccccggatac gcttccgctt cctgaatcgc tttcaccgtc tggttgagct gcgcgccgag
      541 agaaatctgc gccacataaa catgaccgta catcatcata ctgacgccaa gatctttacg
      601 cgctttacgt ttgccgtgct cgccaaattt agttactgca cccagcggtg tcgctttcga
      661 cgcctgacca ccggtgttgg aatagcattg cgtatccagc accagaatgt tgacgttttc
      721 cgtcaaactc aatacatgat ccagaccgcc aaagccgata tcgtaagccc agccatcacc
      781 accaatcagc cagattgatt tttccaccag tgcatctgca tcacgtagca gttcatgtgc
      841 ttcggcaaca tcgttgagtt gctggcgtaa agctgcgacc tgttctcgac gaacctctgg
      901 cgtagcgtct gatttcaacg ccgtcagtaa ttccgccggg attttatcgg caaattgatc
      961 cagcagacgc agcacgcgga cacggtgttg atcgaccgtc aggcggaaac caaggccaaa
     1021 ttcggcatta tcttcaaata gagagttcgc ccatgccggc ccacgaccgt tggcatcggt
     1081 ggtatacggt gtagagggca ggttaccgcc ataaattgaa gaacagccgg tggcgttagc
     1141 gatcaacatc cggtcgccat agagctgagt cagtaattta atatacggcg tctcgccaca
     1201 accggagcaa gcacctgaat attcaaacag cggtgtaatc agctgcgatg tacgaatatc
     1261 aatacgttcc agtttgctac ggtcgatttc tggcaggttg aggaagaaat cgtaattgat
     1321 tttctcttct tcgacatgtt ccaggcgcga catcatattg atggctttaa tctctggatt
     1381 ctgacggtct ttcgccgggc aaacttcgac gcacaggtta caaccggtgc aatcttccgg
     1441 tgccacctgc aagacatatt tctgcccgcg catatcacgc gatttcacat ccagcgaatg
     1501 caggctggca ggggcgtttt ccatcgcttc aggcggcacc acttttgcgc gaatagctga
     1561 gtgtgggcaa gcggcaacgc agtggttaca ttgggtacag agttcctctt tccagatggg
     1621 gatctcttcg gcgatattgc gtttttccca gcgagtggtg cccatcggcc aggtgccgtc
     1681 tggcggcagc gccgaaacgg ggagggcatc accaagccct gcgagcatcg ctgcggttac
     1741 ggttttcacg aaatcagggg cggcatcgga aaccactggc ggtcgattgg cgctgtgcgg
     1801 atttaccggt tgcaacggaa cttcttctac ggattcacgc gccagcgcca gagcctgcca
     1861 gttgcgttcc accagatcct ggcctttgct actgtaactt ttggcaatcg caccctgcaa
     1921 ttctgcgagg gcgctatcgc caggcagaat ttgcgtcaga tggaaaaaag ccatctgcat
     1981 gacggtatta atacgggccg ccaggccaca ttcgcgggcg attttcgccg cgttaatcac
     2041 atagaagcgc gcttttttct gatttagcac ggcctgaact tcctgcggca agcgcgacca
     2101 cacttcatct gcgctgtacg gcgtgttgag caggaaaatg ccgccaggtt ttaaacgctc
     2161 agccatctga tatttatcga taaactgcaa ctggtggcag ccaacaaaat cagcctggga
     2221 aatgagataa gcggaacgaa tcggctgttc gctcacgcga aggtgagaaa ccgtcaggcc
     2281 gcccgccttt ttggagtcgt aaacaaaata gccctgtgcg taccacggcg tggaattacc
     2341 gataatcttg atattgtttt tggtcgcgga aacgctgcca tcactaccaa ggccataaaa
     2401 caaggcttcc agtttcgccg agtttggcag ggtgttttcc ggcaacggca gtgacagatt
     2461 ggtcacatca tcgtaaatac caaccgtaaa gcgcgctttc ggtttagccg cgttgagctc
     2521 ggcaaatacc gccagtacac agtccgggcc aaattctttg gatgaaagac catagcgccc
     2581 accaatgaca cggggcagag tttcgcgctc gccattatta aaggcttctg ccagtgcggt
     2641 cattacatcc agatagagcg gttctgcctg ggcaccgggt tctttggttc tgtccagtac
     2701 cgccacgctg cgtacggatc ccggcagagc ttgcagcaag tgtttggcgg agaagggacg
     2761 gtacaggcga actttcagca cgccaacttt ttcgccacgg gttagcaatt catcaaccac
     2821 ttcttcacag gtgccaatgg cagagcccat caggataatc acccgttccg cttgcggatg
     2881 cccgtaatat tcaaacggct gatactgacg acctgtcgcg gcagagaaat cattcatcgc
     2941 ctgttcaaca tggtcataga ccgcgtcgta ccatgggttg gtggcttcgc gagactggaa
     3001 ataagtgtca ggattggcgg acgtaccgcg gatcaccgga tgttccgggt tgagcgcccg
     3061 ggcgcgatga gcatcaattt cggcctgcgg catgagatca agaatagtgt catcggccag
     3121 cgggacaatt ttattgattt cgtgggacgt gcggaaacca tcaaagaaat gaataaatgg
     3181 cacgcggctc ttcagcgttg cgatgtgcga aatgagagca aaatcttgcg cttcctggac
     3241 gttagccgca cacaacatcg cgcaacccgt ctggcgcacc gccatgacgt cggaatgatc
     3301 gccaaaaata gagagtgcat gtgtggcaac ggtacgtgcc gctacatgca ggacaaacgg
     3361 tgtgagttcg cctgccagtt tgtacagcgt cgggatcatc agcagcaaac cctgcgatga
     3421 cgtaaacgat gttgaaaggg cacccgtctg caaagcgcca tgcacggtag cgatagcacc
     3481 cgcttccgac tgcatttcaa ccacgcgtgg tgtgtctccc caaacgttct tcaagccgtt
     3541 tccggcccag gcatcagcct gttctgccat cgtggaactg ggggtaatag ggtagatggc
     3601 gataacttca ctggtgcgaa acgccaccga agcaaccgcg ccattaccgt caatagtaat
     3661 catatgacac ccttacattg cgcaaatgag gggcgcacga aattgctgcg cgcccagtag
     3721 taatccttca attttagcaa atggctttct tctgcatttt cgcttttgtg tcccccacat
     3781 cagcgtaatg aatgttttga tcaaacagag ggcaaaaaaa tagccataaa aaagtaaaaa
     3841 tgcggggcaa cgcgcaggac agctctcgac gccgccctct gtgctgccta ttatttatgg
     3901 cggtgtcgtt tggcttgaag atcagaggaa gaagagatgc gagcagcgtt ttgggtaggg
     3961 tgtgccgctt tattgttgtc ggcgtgtagt agtgaacctg ttcagcaggc gactgcggcg
     4021 cacgtagcgc caggtttaaa agcgtcgatg tccagtagtg gagaagcaaa ttgtgcaatg
     4081 atcggcggtt cgctttctgt tgcccgtcaa ctggatggta cggcgattgg gatgtgtgca
     4141 ttacccaacg gcaaacgctg tagcgaacag tcacttgccg ccgggagctg tggcagctat
     4201 taattcatta aatccgccag cttataagtt aatgtctgtt tcgcggtcgc cagcgttaac
     4261 tggttcgcgg tcagatccac ttgtgcacct tctttcagca tttcgctaat ggtgttatcg
     4321 agttcattaa gctgcgggtt agcgcacatc atacgggtca ttgccagccc tttggctgtc
     4381 agttcaccat tagacagttt gccttcaccg ctaaagcggt tacacatgct gccggaaatc
     4441 atcatttttt caccaaagct gatttctggc ggatttttat cgctggtcac gggcttaccg
     4501 tttacgcttt ccagcacaaa gcgatgatgc tgtagctgtt ctggcgtaac agcaattttg
     4561 tcattactta cacatcccgc catcagcagg cttagcgcaa caaacgcggc tactttcttc
     4621 attgtggttc tcaattacag tttctgactc aggactattt taagaataga gaatgaaagg
     4681 tcattgggga ttatctgaat cagctcccct ggattgcagg ggagcggcaa gattaaacca
     4741 gttcgttcgg gcaggtttca cctttttcca gattgcttaa gttttgcagc gtagtctgag
     4801 aaatactggt cagagcttct gctgtcagga atgcctggtg cccggtaaac agcacgttgt
     4861 ggcaggcaga caggcgacgg aatacgtcat cctggatcac gtcgttggat ttatcttcaa
     4921 agaacagatc gcgttcgttc tcatacacgt ccatacccaa cgaaccaatt ttctgatttt
     4981 tcagcgcttc aattgctgcc tgagaatcaa tcaatgcacc gcgactggta ttgacgatca
     5041 tcacgccatt tttcatctga tcgaaggcgg cttcgttcaa cagatggtag ttttccggtg
     5101 tcagcgggca gtgcagagag ataacgtctg attcagagaa cagggttggc agatcgacat
     5161 actccacacc gagttccagc gccgctgcac ttggatacgg atcgaacgcc agcagacgca
     5221 taccaaaacc tttcagaatg cgcagcatcg ccacaccgat tttaccggta ccgataacgc
     5281 ctgccgtttt gccatacata gtaaagccgg tcagaccttc cagagagaag ttagcgtcac
     5341 gggtacgctg atacgcgcgg tgaatacggc ggttcagcgt catcatcata ccgatggcgt
     5401 gttcagcaac ggcctctgga tcataggctg gaacacggac tactttcagc cccagttctt
     5461 ttgccgcgtc aaggtcgacg ttattgaaac cggcacagcg cagggcgata tatttaacgc
     5521 cgtgcttttt cagctcttcc agcaccgggc ggctgccgtc atcgtttacg aaaatacata
     5581 ccgcttcgca gccattggca gttttagcgg ttttttccgt cagcagaaag tcaaaaaatt
     5641 ccagctcaaa gccaaaggac tcgttcacct gttgcaggta cttcttgtcg tactgttttg
     5701 tgctataaac ggcgagtttc ataagacttt ctccagtgat gttgaatcac atttaagcta
     5761 ctaaaaatat tttacaaaat ttcaaattta attgaaagct atggcgatat tgaaaaattc
     5821 atcaacaact atgcttagtg taggcgcaac cttcaactga acggttaaac atgccacaat
     5881 acccgtattg aatgcttaat ttttcgctaa atcaggatat taactaccca tgctgggtaa
     5941 atataaagcc gttctcgcgc tgttattact gattattctt gtgccgttga cgctgttgat
     6001 gacgctcggg ctgtgggttc ccacgctggc gggcatctgg ctaccgctcg ggacacgtat
     6061 tgcattagat gaaagcccac gcattacgcg taaaggttta atcattcccg atctccgtta
     6121 tctggtggga gattgtcagc ttgcgcatat caccaacgcc agcctttcac atcccagccg
     6181 ctggttattg aacgtcggca cggtagaact tgattctgct tgtctggcga aattgccgca
     6241 gacggagcaa tcgccagctg ctccaaaaac cctcgcgcag tggcagtcca tgctgcctaa
     6301 cacctggatc aatatcgata aactgatttt ttctccctgg caggaatggc agggaaaact
     6361 ctctctcgca ttaacctctg atatccagca actgcgttat cagggcgaaa aagttaaatt
     6421 tcaaggccag ctgaaagggc aacaacttac agtcagcgaa ctggatgtcg tcgcgtttga
     6481 aaatcagccg ccggttaaac tggtggggga atttactatg ccgctcgtgc cggatggact
     6541 tcctgtaagt gggcatgcta ctgcgacgtt aaacttgccg caggaaccgt cactggtgga
     6601 tgcagagctg gactggcagg aaaatagcgg gcaattgatt gtgctggcac gggataacgg
     6661 cgatccgttg ctcgatttgc cgtggcaaat tactcgtcaa caattgaccg taagcgatgg
     6721 tcgctggagt tggccgtatg caggttttcc tttgagtggc cgactgggtg tcaaagtcga
     6781 caactggcag gcagggcttg agaacgctct gatcagcgga cgactgagtg tgctgaccca
     6841 ggggcaagcg ggtaaaggca acgcggtgct aaattttggc ccaggaaaat taagcatgga
     6901 taacagtcag ctgcctctgc agctgaccgg tgaagcgaaa caggcggacc tcattttata
     6961 tgcccgttta cctgcgcagc taagtggaag tctgtctgac ccaacgctga cctttgagcc
     7021 aggcgcgtta cttcgttcaa agggaagagt catcgattcg ctggacatcg atgaaatccg
     7081 ctggccttta gcgggtgtaa aagtcaccca acgtggtgtt gacggacgtt tgcaggccat
     7141 tttgcaggcg catgaaaatg aactgggcga tttcgtgctg catatggatg ggctggcgaa
     7201 tgattttctc cctgacgcgg gccgctggca gtggcgctac tggggaaaag gcagttttac
     7261 accgatgaat gccacctggg atgtcgcagg aaaaggtgaa tggcatgaca gcacgattac
     7321 gctgaccgac ctctccaccg gtttcgacca gttacaatac ggtacgatga cggtagaaaa
     7381 gccgcgatta attctcgaca agcccgtcgt ctgggtacgt gacgcacagc atccctcctt
     7441 cagcggcgcg ctgtcactgg acgccgggca aacgctgttc actggcggca gtgtgttacc
     7501 gccatcaacc ttaaaattta gcgtcgatgg gcgcgatccg acgtatttcc tctttaaagg
     7561 cgatttacat gctggtgaga ttggcccggt tcgggtaaat ggtcgctggg acggtattcg
     7621 tctgcgcggt aacgcctggt ggccaaaaca atcactgact gtattccagc cgctggtgcc
     7681 acccgactgg aagatgaact tacgcgatgg tgaactgtat gctcaggttg cattttctgc
     7741 tgcgccggaa caaggattcc gcgcgggagg gcacggcgtg ttgaaaggcg gtagtgcctg
     7801 gatgccagat aatcaggtta acggtgtcga ttttgtcctg cctttccgtt ttgccgatgg
     7861 agcctggcat ctggggactc gcggccccgt tacgttgcga attgccgaag tgattaatct
     7921 ggtgacagcg aaaaatatta cggctgattt gcaagggcgt tatccgtgga ctgaagaaga
     7981 acccttgctg ttgactgatg ttagcgtcga tgtgttaggc ggtaacgtac tgatgaaaca
     8041 attacgtatg ccgcaacatg acccggcgct gttgcggctg aataatctct catccagcga
     8101 actggttagc gccgtcaatc cgaaacaatt cgccatgtcc ggggcattta gtggtgcatt
     8161 gccattatgg ctaaacaacg aaaaatggat agtgaaagat ggttggctgg caaatagcgg
     8221 gccaatgacc ttgcggctgg ataaagacac cgcagatgcg gtggtgaaag acaatatgac
     8281 tgcgggttca gcaattaact ggttgcgcta tatggaaatt agccggtcat cgacaaaaat
     8341 taacttagat aatctcggtt tattaaccat gcaggccaac attacaggta ccagtcgcgt
     8401 tgatggtaaa agcggtacgg taaaccttaa ttaccatcat gaagagaata tttttacgct
     8461 gtggcgcagt ttacgctttg gcgataatct ccaggcatgg ttggagcaga acgcacgtct
     8521 gccgggaaat gactgtccgc aaggaaaaga gtgtgaggaa aaacaatgaa aattttactg
     8581 gctgcgttga cgtcatcttt tatgctggtt ggctgtacgc ctcgcattga agtcgctgca
     8641 cctaaggaac cgatcactat caatatgaac gttaaaattg agcatgagat catcatcaag
     8701 gcagacaaag atgtcgaaga gctgcttgaa actcgtagcg atcttttctg aggcgatgat
     8761 gaagaaaaca ttacttcttt gtgcgtttct tgttgggctg gtaagcagca atataatggc
     8821 attgactctg gatgaagcca gaactcaggg gcgggtaggt gaaacatttt atggttatct
     8881 ggttgcgctg aaaacggatg ctgaaacaga gaaattagta accgacatta atgccgaacg
     8941 taaagcgagt taccaacaac tggcaaagca aaataatgtg tcggtagatg atatcgcgaa
     9001 actcgccggg caaaagctcg tagctcgggc caaaccgggg gaatatgtac aagggataaa
     9061 cggtaaatgg gtgcgaaaat tttaatagca aaaccgcaat atcgggataa tgctacagga
     9121 gaaagcgatt gatctatttt cctgaaacaa ggtgaatatt aaaaaactcc tgttaaattg
     9181 ccttttgccc tgaaaaatgc atagtcaaat atctgtttta actaattggc gttgcagtac
     9241 atgcaacgcg aattagttag cggaatttac gccgatactc gcctggcgtc atcccaaagc
     9301 gttgcttaaa tactgttgaa aaatgactct ggtcggaaaa tccccaatga aaaccaatgc
     9361 ctgccagttt ttcatcatct gcggcatggc gaatcgcatc agcacaaaaa tcgagacgac
     9421 ggttacgaat atattgcgcg accaccaaac ctttatcggc aaacattcgg tacaaactac
     9481 gtactgacat acctgtctct ccggctatcc actccgggcg taatatctct tcgcgaatat
     9541 tatcgtctat cagcgtaacc actttttgaa actgacgttc acggcgaggt tgaacagatt
     9601 cccgctgatg aagtaccggg cgcagcagac acaccatcgc ctgtagcgca gcttcacttt
     9661 ctgtttcaga aagtgccggg ttattcatgc tctcctgtaa caggcgatga ctgagttgca
     9721 ccatgggtaa gtcagcgtcc agtctttctg cgcagacagg tttttgatgg ggaaaatatt
     9781 gttccagcag agtgcgtggt aaaagtaatg aaatctgttt agaagactcc tgccagtaaa
     9841 gcgaacaggg gcgtgaggca tcgagtaacg taatatcgcc agcgccaatc tgcacctgac
     9901 gctcatcctg ctccattatt gcctgaccac taagttgaaa aacggtgtaa aaccaggcat
     9961 cgtcgctgcc ttttacctcc tgccaggtgc ggtataaatt cactccgttg gttgtcacgg
    10021 tgctcagctt tagtcctttg gcaaaatgcg tttccagtac acccgtgtaa cgctcagtca
    10081 gcaggcgtcc ggtaaaattt ccgcatacct gattgatttg ggaaagccat tgctgaaact
    10141 cattatccac tgcggggttc atggcacgtt ttcgctctgt gaaatgtatt tttattgttg
    10201 catttgtgtt gcaataaacg aagctaatga gcctgactat aggaaataag tcttgtcagg
    10261 catagagaca taagcggtta ttgtcacgat ttgcggagct tgtcacagct gacaaagcga
    10321 atgtcacagc gaaaaaagtg acttttcttg tcgctgcgta cactgaaatc acactgggta
    10381 aataataagg aaaagtgatg acagagccgc atgtagcagt attaagccag gtccaacagt
    10441 ttctcgatcg tcaacacggt ctttatattg atggtcgtcc aggccccgca caaagtgaaa
    10501 aacgattggc gatctttgat ccggccaccg ggcaagaaat tgcgtctact gctgatgcca
    10561 acgaagcgga tgtagataac gcagtcatgt ctgcctggcg ggcctttgtc tcgcgtcgct
    10621 gggccgggcg attacccgca gagcgtgaac gtattctgct acgttttgcc gatctggtgg
    10681 aacagcacag tgaggagctg gcgcaactgg aaaccctgga gcaaggcaag tcaattgcca
    10741 tttcccgtgc ttttgaagtg ggctgtacgc tgaactggat gcgttatacc gccgggttaa
    10801 cgaccaaaat cgcgggtaaa acgctggact tgtcgattcc cttaccccag ggggcgcgtt
    10861 atcaggcctg gacgcgtaaa gagccggttg gcgtagtggc gggaattgtg ccatggaact
    10921 ttccgttgat gattggtatg tggaaggtga tgccagcact ggcagcaggc tgttcaatcg
    10981 tgattaagcc ttcggaaacc acgccactga cgatgttgcg cgtggcggaa ctggccagcg
    11041 aggctggtat ccctgatggc gtttttaatg tggtcaccgg gtcaggtgct gtatgcggcg
    11101 cggccctgac gtcacatcct catgttgcga aaatcagttt taccggttca accgcgacgg
    11161 gaaaaggtat tgccagaact gctgctgatc acttaacgcg tgtaacgctg gaactgggcg
    11221 gtaaaaaccc ggcaattgta ttaaaagatg ctgatccgca atgggttatt gaaggcttga
    11281 tgaccggaag cttcctgaat caagggcaag tatgcgccgc cagttcgcga atttatattg
    11341 aagcgccgtt gtttgacacg ctggttagtg gatttgagca ggcggtaaaa tcgttgcaag
    11401 tgggaccggg gatgtcacct gttgcacaga ttaacccttt ggtttctcgt gcgcactgcg
    11461 acaaagtgtg ttcattcctc gacgatgcgc aggcacagca agcagagctg attcgcgggt
    11521 cgaatggacc agccggagag gggtattatg ttgcgccaac gctggtggta aatcccgatg
    11581 ctaaattgcg cttaactcgt gaagaggtgt ttggtccggt ggtaaacctg gtgcgagtag
    11641 cggatggaga agaggcgtta caactggcaa acgacacgga atatggctta actgccagtg
    11701 tctggacgca aaatctctcc caggctctgg aatatagcga tcgcttacag gcagggacgg
    11761 tgtgggtaaa cagccatacc ttaattgacg ctaacttacc gtttggtggg atgaagcagt
    11821 caggaacggg ccgtgatttt ggccccgact ggctggacgg ttggtgtgaa actaagtcgg
    11881 tgtgtgtacg gtattaatct ggttcgctca taagtaaaaa acggcacctg gtgccgtttt
    11941 tttgtctgaa acaatcactt atctttcttc agcgccccta gcgttggcgt ttcgtcaaag
    12001 aagttccatg gtttaagcag agtatgtacc cattcggtcg gcataatcgg ccactcttcg
    12061 gcgcgggcca catgtgtggt gccggtggtc atccagacaa cggcgtcggt gttgtccagc
    12121 gactcgttat ctttactgta ttgtccaaga ccggtgtcat gagtagaacg gttcggatat
    12181 ttgccttccg ggaaacgctc gccaggatga taacgcgtta cccaaagctg cttgtccata
    12241 aagcttaaac gatgatagat ccactcgtcc ggcgcgaact gggcaccttt tgctaccggg
    12301 tgagtaccac ctgcataagg aataatttga taggaaactg gattgcccat gcggttctct
    12361 ttgttcgggt tactcaacag acgaatcgtg cccggatcaa acttctgcgc ggcatcctgt
    12421 tcattgccga tgttgtactg attaacttgc atggtactgg tgcgtgggcc accggcagta
    12481 ttcggtttta ccactgggtc catcgccacc aggctgttat tctcgccatc tacatccaga
    12541 tcgaggcgga aattataaat atgttggtgt gtagtaccca cgatattgtg atcgataagc
    12601 gtgccgtagc gcgtgtcatc tttcgccgtc tcatcgtgca tggttttcgc tttaacacct
    12661 ttcaccgctt cgatgcccgt agcaccggca tcaatgccaa tagtgccgtt ttcatggaag
    12721 atccagtcaa aaatgtagtc atagttaccc actgtactga tccagcgcac cactaactcc
    12781 cggcgttcgg tactgacgtt gggctggccc atttcctgat gcttatactc cggcccggca
    12841 taacgttcaa ataccgcgat agcgcgaggg atctccatcg gcacgccagt gtagtcggcg
    12901 atggtttcat taaggagcac tgcgttagac ggggcatctt taccacgagc aattggtgag
    12961 gttagcgtgc ccataccgta gtcaccagag tccagatacg ctttaaagta ccagccaata
    13021 tcaggatcac cgtaaggcac aatcatgccg ccgagagaac cttcgtacat gactttgcgt
    13081 ttggtgccat tgtcgttata agtcacggtg gagatcatcg gcccgacgcg agagttcatg
    13141 ctgaggtgaa aatcccagtt ccgccagtga atcatatcgc cagtaatggt gtaattttta
    13201 ccttcaggct caatgatttg cataggctta actgccggag caacgcggtc acggctatca
    13261 aatgggcgtg cggtcattgg caccggaact accggacctt cttcaatctt aacgattttt
    13321 ttctgttcta aatcaacgac cgccaccagg ttttcgatgg gatgtgccca gtagttgcca
    13381 tcaccgacat caagatagct gatgactttg agcaaccggg catcttgttt caggccatct
    13441 ttaccatcga aataacctac ggtcagcggc gtggtaatca cttttttcgc atcagtaata
    13501 ccgcgtttct tcacggcagc ggcaaattct tcactgttgt taataatgtt ctgcacactg
    13561 gcgaaatcat ccagcaacac cataccgtgg gcgtctttag tgggttgcca ggagagcagt
    13621 ttgttgtttt gcagatccac caccgcttcg atgatatgtt tgccgtcgag cataatgacg
    13681 tcggctttgc gcggctggtc aaccggtttg ttttccagcg caaacgccca gacagcttct
    13741 ttatctggcg gtagcaggga gatctcagta aaacgggtat tgggtttgaa gtccgcggaa
    13801 gctttaacaa tttcaacggc ctgtttaatt tcgtccgcag ttagcgcatt aagtgggtga
    13861 gggcgctttt ctacctgaaa ggtttgatcc agcccggact ggaaaacatc gttaataaag
    13921 gtgtcagaaa cccaggcttt attgtctttc atcactaccg gtacttgcag tgccagaggc
    13981 tgaccattaa caattgctgt ttgcgcacca ggcttcactt tcacgtacgc gccatcttta
    14041 atcagggtaa agagctgggc gtagtcgtcc cactgcacat cggcaccaaa ttctttaagc
    14101 gttttatcca ttggcaccat atgcgcttca ccaccgtggg caaataccgg cgcttgccag
    14161 gcgaaactta aggcgactgc caacgccagg gttgttttac gggcagaata cagagagggg
    14221 cttcccatta ttaacctcgt cagatgttgt gttcttgtta gcaaccgcgc tctgtgggcg
    14281 gtttagtcag gttcacatta tcagtactga tgcaaagggg attgcctgca cctgccaggt
    14341 tgtttggcag gtgtgccagc ttttcataca gtggatgccc tgaaaataga tgtacacatc
    14401 atgcataatg tgacaacgtc acaaaattta gtgaaataaa agggcaacta ttcgccgttg
    14461 cccttcattc accgattaat cgacaaaatc accgtgctgc ctggccacca gcgtcagaat
    14521 tgaatacagc gccaccgggg tttgatgctg attgaatacc tctaccgccc attccaccac
    14581 acctgttggt ttttcttctg cgctacgctg ttttttcagc gtcttgcgct tacaggtgag
    14641 acgcacctgg atggtatcgc ctggctttac gggttcgata aaacgcaagc tttccagccc
    14701 gtagttagca atgaccggac caacaccggc atcgacaaac agacccgcag ccgcagaaag
    14761 cacaaaatac ccatgcacca cccgctcacc gaaaatagat tcggcagcag caatcttatc
    14821 catatgtgca tagaaatgat cgccgctgag gcaagcaaag ttaacaatat cggcctctgt
    14881 cattgtgcgg cggggagtca acaggctgtc gcctggttgt agctcctcaa aatatttgcg
    14941 gaacggatga atacgatctt cttcgacttt cgcaccgcgc acccactgtt tactgatagc
    15001 ggcaagcatc gtcggactac cctgaacagc ggttcgctgc atgtaatgtt tcaccgctcg
    15061 taaaccgcct aattcttcac cgcctcctgc gcgaccaggc ccaccatgta ccagttgtgg
    15121 cagtggggag ccatgcccgg tggattcttt tgccgattct tcattgagga tctgaattcg
    15181 cccatgcgta cgtgccgcgt cggcaataaa ctgacgcgca atttgcggat cagccgtcac
    15241 cagcgttccc gcaaggctac cgccgcctgc acaagccagt tgcagagcat gttgctggtt
    15301 ttgtgctggc attagcgttg cgacagggcc aaaggcttct gttgcatgta ccgccggtgt
    15361 ttcatccggc tgcggacagt acaataaggt tggcgggaag aaggcacccg ccgcagataa
    15421 atccgcctga ccaccgaggc gaatctcgca tcctgcagcc agcaatgtgt tcactttttc
    15481 ctgcacatca gcacgctgtt cagcatttac cagtgcgccc attttcacgc cttcttgtgc
    15541 aggatcaccg accacgactt tctgtaatcg cgcaaccaga gcatcactga cagcattaac
    15601 caatgtctgc ggcacaataa tccgccggat tgccgtacat ttttgcccgg cttttgtggt
    15661 catctcacgc acaacttcac gaataaacag cgcaaactcc ggttgatccg gggtgatatc
    15721 ttcgcccagt acgcagcaat tcagggaatc agcttccatg gtgaagggga tagatttggc
    15781 tacgatattt ggctgaactc gtagcatctg tccggtcgtc gcggaccccg tgaaagtcac
    15841 cacatcctgg ctgtccagat gatccaaaag gtcgcccgca ctaccgcaga tcagactaat
    15901 tgcgccttcg ggaacaagac cactatcgac aattgatttc accatcgcct gagtcagttg
    15961 ggccgtcgcg gtagctggtt tgatgatggc tggcattccg cccagccacg ttggtgccag
    16021 cttttccagc attccccagc aggggaagtt aaaggcgtta atatgcactg ccacgcctga
    16081 ctttgaggtc agtacatggc gcgcggcaaa tccaccttct ttcgataagg ggagcaattc
    16141 atcttccggc cacagcgtat cgtcaggcag ctcccggcta ccgaggctgg cgtaagtaaa
    16201 taacgtccca atgccacctt caatatcaac ccaactgtct gcccgcgttg cgcctgtttg
    16261 cgcagaaaga gcatagaaac gctctttttc actcagcaga tgtttagcga ccgctttaag
    16321 catcgccgca cgttcgataa aggtcatagc gcgaagggct ggggcacctt tttcaatggc
    16381 aaacaggcgg gcagccgcca tatcaagacc ttcactggtc acttcccata acgcctcgcc
    16441 gctaatagcg tggtgaatca aacggctacg gccccggcca gactgccagg taccggataa
    16501 gaaactggct aactgctgca tcgctactct ccagatgttt cacatttctg ttgctaatag
    16561 ttgaatcgcg aatcataaaa agcaagggat cttttaatga aatgttaact atgcgatctg
    16621 tatagcaact gcggaaaaca ttaatgcaat gataaataat gatttataaa aagagggtgc
    16681 gaaatccgtc acagttcaag catacaaaat ttgtgatttt agttaaccat tgtgtaactt
    16741 tcataaaaca atgtgattcg tgtttttaat taattcacga aaactggaat cgtaaaggtg
    16801 atgacgtgac ccaagaagaa cgctttgagc aacggatagc ccaggaaacg gctatcgagc
    16861 cacaggactg gatgcccgat gcttaccgaa agacattgat ccgccagatt gggcagcatg
    16921 cgcactccga gattgttggt atgttgcctg aaggtaactg gatcactcgc gcaccaacct
    16981 tgcggcgtaa agccattctg ttagccaaag tgcaggatga agccggtcat ggtctgtatc
    17041 tctatagcgc cgccgaaaca ctgggctgtg ccagagaaga catctaccaa aaaatgctcg
    17101 acggacggat gaaatattcc tccatcttta attatccgac attgagttgg gctgatatcg
    17161 gcgttatcgg ctggctggtg gatggcgcag cgatcgtcaa tcaggtggcg ttatgccgaa
    17221 cttcttatgg cccgtatgcc agagcaatgg tgaaaatctg caaagaagag agttttcacc
    17281 agcgtcaggg gtttgaagcc tgcatggcac tggcgcaggg cagcgaagct caaaagcaga
    17341 tgttgcaaga cgccattaac cgtttctggt ggccagcctt aatgatgttc gggccaaacg
    17401 atgataactc gccaaacagc gccagaagtc tcgcctggaa aatcaaacgt ttcaccaacg
    17461 acgaactccg ccagcgtttc gtggacaaca ccgttccaca ggttgaaatg ctcggtatga
    17521 ccgttcctga cccggatctg cattttgaca ctgaaagcgg tcactaccgc tttggtgaga
    17581 tcgactggca ggagtttaac gaagtaatta acggtcgcgg aatttgtaat caggagcggc
    17641 ttgacgccaa acgtaaagcc tggaaagaag gtacctgggt acgggaagca gcgctggccc
    17701 atgcacaaaa acaacatgcc cgtaaggtcg cataaggaga ttcaaaatga gtaatgttta
    17761 ctggccgtta tacgaagttt tcgtgcgtgg caaacagggc ttatcacacc gccatgttgg
    17821 cagtttacat gctgccgatg agcggatggc actggaaaat gcccgtgatg cttacacccg
    17881 tcgtagcgaa ggatgttcaa tttgggtggt gaaggcgagt gaaattgttg cctcgcaacc
    17941 ggaagaacgc ggtgaatttt ttgatccggc tgaaagcaag gtctatcgcc atccaacgtt
    18001 ttacaccatc cctgatggca ttgagcacat gtgaggtcgg aaatgaatca gttaacggct
    18061 tacaccttgc gcctgggcga taactgcctg gtgctctccc agcggttggg tgaatggtgc
    18121 ggtcatgcac cggaactgga aatcgatctc gcactggcaa atattggact cgatttatta
    18181 ggccaggcac gcaacttctt atcgtatgcc gctgaattag cgggagaagg cgatgaagat
    18241 accctggcct tcacccgaga cgagcgccag ttcagcaact tattgttggt tgaacagcca
    18301 aacggcaatt ttgccgacac cattgcacgc cagtatttca tcgatgcatg gcatgtggcg
    18361 ctctttaccc gtctgatgga gagccgtgat ccgcaactgg cggcgatttc tgccaaagca
    18421 attaaagaag cgcgttatca cctgcgtttt agtcgtggct ggctggagcg actgggcaat
    18481 ggtactgacg tatcagggca aaagatgcag caggccatca acaagttgtg gcgttttacc
    18541 gccgaactgt tcgatgccga cgagattgat attgcactga gtgaagaggg tattgcggtt
    18601 gatccacgca ctttacgcgc agcgtgggaa gccgaagttt ttgccgggat caacgaagcc
    18661 acattgaacg taccgcaaga gcaggcgtat cgcactggcg gtaaaaaagg actgcatacc
    18721 gaacaccttg gacccatgct ggcagaaatg cagtatctcc agcgtgtctt gcccggtcag
    18781 caatggtaac agaggagatg ggtatgcaac gtctggccac cattgcaccg ccacaggttc
    18841 atgagatatg ggcgctactc agccagatcc cggacccgga gatcccggtg ctgaccatta
    18901 ccgatttagg catggtgcgt aatgtgacac agatgggaga aggatgggtg atcggcttta
    18961 cgccgactta ttccggttgt ccggcaacgg aacatttgat tggcgcgata cgtgaggcaa
    19021 tgacaaccca cgggtttacc cccgttcagg ttgtgctgca actcgacccg gcatggacca
    19081 ccgactggat gacccccgat gcccgtgagc gtctgcgaca gtatggcatt agcccgcccg
    19141 ccggacacag ttgccatgcc catttgccgc cagaagtacg ttgcccgcgc tgcgccagcg
    19201 tccataccac acttatcagt gaatttggtt ccacggcctg caaagcattg tatcgctgcg
    19261 atagttgccg cgaacctttc gattatttca aatgtatttg aggatgccat gacaacgttt
    19321 cattccttaa cggtggcaaa agtggagccg gaaacccgtg atgcggtgac cattaccttt
    19381 gcggtgcccc agcctttgca ggaggcgtat cgctttcgcc ccggtcaaca tttgacctta
    19441 aaagccagct ttgatggtga agaattacgc cgttgttact ccatttgccg cagctatctg
    19501 cctggcgaaa ttagtgtggc ggtgaaagcc attgaaggcg gacgtttctc ccgctatgcc
    19561 cgcgaacaca tccgccaggg tatgacgctg gaggtcatgg tgccgcaggg gcatttcggc
    19621 tatcagccac aggccgaacg ccagggccgc tatctggcta ttgcagcagg atcaggtatt
    19681 acgccaatgc tggcgattat cgccaccact ttacaaaccg agcctgaaag tcagttcacc
    19741 ctgatctacg gtaaccgtac cagccagagc atgatgtttc gccaggcact ggcagacctg
    19801 aaagacaaat atcctcagcg tttacagttg ttgtgcattt tcagtcagga aaccctcgac
    19861 agcgatctgc ttcacgggcg tattgacggt gaaaaattac agtcacttgg ggcctcgctc
    19921 attaattttc gtctttatga tgaggcattt atttgtggtc cggcggcgat gatggatgag
    19981 acggaagccg ccttaaaagc actgggaatg ccagataaaa ccattcatct ggagcggttt
    20041 aatacgcctg gcacgcgcgt caaacgtagc gttaacgtgc aaagtgacgg acaaaaagtg
    20101 actgtacgtc aggatgggcg ggatcgggaa atcgtgctta atgccgacga tgaaagcatt
    20161 ctcgatgcgg cattgcgcca gggggctgat ctgccctatg cctgcaaagg cggcgtctgt
    20221 gcgacctgca aatgcaaagt gctgcgtggc aaagtggcga tggaaaccaa ttacagtctg
    20281 gaaccggatg aactggccgc aggctatgtg ttgagttgcc aggcactgcc gctgaccagc
    20341 gatgtggtgg ttgactttga cgcgaagggg atggcatgag cgaactgatc gtcagccgtc
    20401 agcaacaagt attgttgctg acccttaacc gtcccgccgc acgtaatgcg ctaaataatg
    20461 cccttctgac gcaactggta aatgaactgg aagctgcggc taccgatagc agcatttcgg
    20521 tctgtgtgat taccggtaat gcacgctttt ttgccgctgg ggccgatctc aacgaaatgg
    20581 cagaaaaaga tctcgcggcc accttaaacg atacacgccc gcagctatgg gcgcgattgc
    20641 aggccttcaa caaacctctc atcgcagccg tcaacggtta cgcgcttggt gcgggttgcg
    20701 aactggcatt gttgtgcgat gtggtggttg ccggagagaa cgcgcgtttt ggtttgccgg
    20761 aaatcactct cggcatcatg ccaggcgcag gaggaacgca acgtttaatc cgtagtgtcg
    20821 gtaaatcgtt agccagcaaa atggtgctga gcggagaaag tatcaccgct cagcaagcac
    20881 agcaggccgg gctggttagc gacgtcttcc ccagcgattt aaccctcgaa tacgccttac
    20941 agctggcgtc gaaaatggca cgtcactcgc cgctggcctt acaagcggca aagcaagcgc
    21001 tgcgccagtc gcaggaagtg gctttgcaag ccggacttgc ccaggagcga cagttattca
    21061 ccttgctggc ggcaacagaa gatcgtcatg aaggcatctc cgctttctta caaaaacgca
    21121 cgcccgactt taaaggacgc taatgatgga attcatcctc agtcatgtag aaaagggcgt
    21181 gatgacacta acgctcaacc gcccggaacg cctgaacagt tttaatgatg agatgcacgc
    21241 acaactggca gagtgcctga aacaggtcga gcgcgacgac actatccgtt gcctgttact
    21301 tacaggtgcc gggcgcggat tttgtgctgg tcaggatctt aacgatcgta acgttgatcc
    21361 cacaggcccc gcaccggatt tagggatgtc agttgaacgt ttctataacc cactggtacg
    21421 tcgcctggca aaactgccaa aaccggtgat ctgtgcagtc aatggcgtgg cggcaggggc
    21481 tggcgcaaca ctggcactgg gatgcgacat cgttattgct gcccgttcag caaaattcgt
    21541 catggcgttt agtaagttag gtttagtacc cgattgcggt ggaacctggt tactgccacg
    21601 cgttgctggg cgagcgcgcg ccatggggct ggcactgctg gggaatcaac tgagtgctga
    21661 acaggcgcac gaatggggga tgatctggca ggttgttgat gatgaaacgc tggcagatac
    21721 cgcgcaacag ctggcacggc atctggcgac acaaccgaca tttggtcttg gacttatcaa
    21781 gcaagcgata aatagcgctg aaaccaatac tctcgatacg caactggatc tggaatgcga
    21841 ctatcagcga cttgccggac ggagcgcaga ttatcgtgaa ggtgtcagtg cgttcctggc
    21901 taaacgctca ccgcagttca cggggaaata gcatgatgat aaatgtgcaa actgtggcag
    21961 tgattgggag cggcaccatg ggggctggca ttgctgaagt tgccgccagt catggacatc
    22021 aggttttact gtatgacatt tctgctgaag cgctgacccg cgcaatcgac gggatacacg
    22081 cgcggctaaa ttcacgcgtg acgcggggaa aactgactgc tgaaacctgt gaacgcacat
    22141 tgaaacgcct gatcccggtg accgatattc acgcgctggc agctgcggac ctggtcattg
    22201 aagcggcgtc tgaacgtctg gaagtcaaaa aagcgctctt tgcacagctg gcggaagttt
    22261 gcccgccaca aacgctattg accactaaca cttcgtcaat ctctataacc gcgattgctg
    22321 cggaggtaaa aaatcctgaa cgtgttgcgg ggctgcattt ttttaacccg gcaccggtga
    22381 tgaagttggt ggaggtggtc agtgggctgg caacggcagc ggaagttgtt gagcagttgt
    22441 gtgaactaac gttgagttgg ggtaagcagc ctgtgcgctg tcattcgacc cctggattta
    22501 tcgttaaccg tgtggcgcgt ccttattatt ccgaggcctg gcgggcactg gaagagcagg
    22561 ttgctgcacc agaagtgatt gacgctgcac ttcgcgatgg cgctggtttc ccgatggggc
    22621 cgctggaatt aaccgatctg attggtcagg acgtcaattt tgctgtcacc tgttcggtgt
    22681 ttaacgcttt ctggcaggag cgtcgttttt taccttcgct ggtgcaacag gaactggtga
    22741 ttggtggacg gttgggcaag aaaagtgggc tgggcgtgta cgactggcgc gcggaacgtg
    22801 aggcagttgt tggcctggaa gcggtaagcg acagttttag cccaatgaaa gtagaaaaga
    22861 aaagtgacgg tgtcacggaa attgacgatg ttttattgat tgagacacaa ggcgagacgg
    22921 cacaggcgct ggcaatacga ctggcacgcc cggtggtagt ggtcgacaaa atggcgggca
    22981 aggtggtgac cattgctgcg gcagcggtga acccggactc agcgacccgc aaggccattt
    23041 attacctgca acagcagggc aaaacagtgc tgcaaattgc agattaccca ggaatgctga
    23101 tttggcgaac ggtagcaatg atcatcaatg aagcccttga tgcgctgcaa aaaggcgtgg
    23161 cctctgaaca ggatatcgat accgccatgc gtcttggggt gaattatcca tatggcccgc
    23221 ttgcctgggg agcgcaactt ggctggcagc gaatattaag gctcctggaa aatctacaac
    23281 atcactatgg cgaagaacgc tatcgcccat gttcattgct gcgccaacga gcgcttctgg
    23341 agagcggtta tgagtcataa ggcctggcaa aatgcccatg caatgtatga gaacgatgcc
    23401 tgcgccaaag cgcttggcat cgacattatc tcaatggatg aaggctttgc tgtagtgacc
    23461 atgaccgtca ctgcacaaat gcttaatggt catcaaagtt gccacggcgg gcagctattt
    23521 tcactggctg atactgcctt tgcctacgct tgcaatagcc aggggttggc agctgtcgct
    23581 tctgcctgca cgattgattt tttacgtcca ggctttgccg gagacacctt aactgcgact
    23641 gcgcaggtac gtcatcaggg caagcaaacc ggtgtttacg acatcgaaat tgttaaccaa
    23701 caacaaaaaa cggttgcgct gtttcgcggt aaatctcacc gcatcggcgg caccattaca
    23761 ggagaagcct gatgcgtgaa gcctttatct gtgacggaat tcgtacgcca attggtcgct
    23821 acggcggggc attatcaggt gttcgggctg acgatctggc tgctatccct ttgcgggagc
    23881 tgctggtgcg aaacccgcgt ctcgatgcgg agtgtatcga tgatgtgatc ctcggctgtg
    23941 ctaatcaggc gggagaagac aaccgtaacg tagcccggat ggcgacttta ctggcggggc
    24001 tgccgcagag tgtttccggc acaaccatta accgcttgtg tggttccggg ctggacgcac
    24061 tggggttcgc cgcacgggcg attaaagcgg gcgatggcga tttgctgatc gccggtggcg
    24121 tggagtcaat gtctcgggca ccgtttgtta tgggcaaggc aaccagtgca ttttctcgtc
    24181 aggctgagat gtttgatacc actattggct ggcgatttgt gaacccgctc atggctcaac
    24241 aatttggaac tgacagcatg ccggaaacgg cagagaatgt agctgaactg ttaaaaatct
    24301 cgcgagaaga tcaagatagt tttgcgctac gcagtcagca acgtacggca aaagcgcaat
    24361 cctcaggcat tctggctgag gagattgttc cggttgtgtt gaaaaacaag aaaggcgttg
    24421 taacagaaat acaacatgat gagcatctgc gcccggaaac gacgctggaa cagttacgtg
    24481 ggttaaaagc accatttcgt gccaatgggg tgattaccgc aggcaatgcc tccggggtga
    24541 atgacggagc cgctgcgttg attattgcca gtgaacagat ggcagcagcg caaggactga
    24601 cgccgcgagc gcgtatcgta gccatggcaa ccgccggggt ggaaccgcgc ctgatggggc
    24661 ttggtccggt gcctgcaact cgccgggtgc tggaacgtgc ggggctgagt attcacgata
    24721 tggacgtgat tgaactgaac gaagcgttcg cggcccaggc gttgggtgta ctacgcgaat
    24781 tggggctgcc tgatgatgcc ccacatgtta accccaacgg aggcgctatc gccttaggcc
    24841 atccgttggg aatgagtggt gcccgcctgg cactggctgc cagccatgag ctgcatcggc
    24901 gtaacggtcg ttacgcatta tgcaccatgt gcatcggtgt cggtcagggc atcgccatga
    24961 ttctggagcg tgtttgagca tatcaacctg cgagtaccct acaatgataa ccaatacaaa
    25021 gcttgacccg attgaaaccg cgtctgttga tgagttacag gcgttgcaaa cacagcgtct
    25081 gaaatggacg ctcaaacacg cgtatgaaaa tgtcccgatg tatcggcgca aattcgacgc
    25141 agcaggcgta catcctgatg atttcaggga actttcagac ctgcgtaaat ttccctgtac
    25201 caccaaacag gatctgcgcg acaactatcc ctttgacacc tttgccgtgc cgatggaaca
    25261 agtggtgcgc attcatgctt cttcgggaac cacaggtaaa ccgacagttg tcggttatac
    25321 gcaaaacgat attgataact gggccaatat tgtagcgcgt tctttgcgtg cggcaggggg
    25381 ctcgcccaaa gacaaaattc atgttgccta tggttacggc ctgtttactg gtgggctggg
    25441 cgcgcactat ggtgccgaac gtctgggcgc tacggtgatc ccgatgtctg gcgggcagac
    25501 ggaaaaacaa gcgcaactga tccgcgattt tcaaccagat atgatcatgg ttacgccatc
    25561 ttattgcctt aacctgattg aagagctgga gcggcagttg ggcggtgatg ccagcggttg
    25621 ctcgctgcgg gttggcgtgt ttggtgctga gccgtggaca caggccatgc gtaaagagat
    25681 tgaacggcgt ctggggatca ccgcactgga tatttatggc ctgtcagaag tgatggggcc
    25741 gggggtggcg atggagtgtc tggaaactac cgacggtcca actatttggg aagatcattt
    25801 ctatcctgaa attgttaatc ctcatgacgg cacaccgctt gccgatggtg aacatggcga
    25861 actgttattc accacgctga ccaaagaagc attgccggtc attcgttacc gcacgcgtga
    25921 tttaacccga ctgttaccag gaacggcgcg gactatgcgc cgcatggatc gcatcagcgg
    25981 acgcagcgat gacatgttga ttattcgcgg tgtaaatgtc tttccgtcac aactggaaga
    26041 agagattgtc aaattcgaac atttatcgcc gcattaccaa ctggaggtga accgccgggg
    26101 gcatcttgat tcactttctg tgaaagtgga gttgaaagaa agtagcctga cattgacgca
    26161 tgagcagcgt tgccaggtat gccaccagct gcgccatcgg attaagtcga tggtggggat
    26221 ctctaccgat gtgatgatcg ttaactgtgg cagtatcccg cgttcagaag gcaaggcgtg
    26281 tcgggtgttt gatctgcgca atattgttgg tgcctgacgt gtcgtctggc cctggtgagg
    26341 taaagcgcca gggccagaag tcgatacgac ctgtgctatg attcataaat cacaacaata
    26401 acaacagact gaatcgaatg agtaaacttg atacttttat ccaacatgct gtaaacgctg
    26461 ttcctgtcag tggcacatct ttgatctcct ctctgtatgg tgattctctt tcccatcgtg
    26521 gtggtgaaat ctggctgggt agtctggctg ctttgctgga agggctggga tttggtgagc
    26581 gtttcgtgcg cactgctttg tttcgtctta ataaagaagg ctggctggat gtttcccgca
    26641 tcgggcgacg cagtttctat agcctcagtg ataaaggctt gcgcctgacg cgacgggcag
    26701 aaagtaaaat ttatcgcgca gagcaacccg catgggatgg taaatggctc ctgttgctct
    26761 cggaaggttt agataaagcg acgctggctg atgtcaaaaa gcagttgatc tggcaaggtt
    26821 ttggtgcact ggcgcccagc ctgatggcat cgccgtcgca aaaactggcc gatgtacaga
    26881 cacttttgca tgaagcgggt gtggcggata acgtgatttg ttttgaagcg caaataccac
    26941 tggcgctttc tcgtgcagca ctgcgtgcca gagtagaaga gtgctggcat ttaactgaac
    27001 aaaatgccat gtacgaaacc tttattcagt cattccgccc gttggtgcag ctgttaaaag
    27061 aggcagcaga cgagttaacc ccggagcgtg catttcatat tcagctttta ctgatccatt
    27121 tttatcgccg tgtcgtcctt aaagaccctt tgttgccgga ggagttgctt ccggctcact
    27181 gggcagggca tacggcgcgt cagctgtgta tcaacattta tcagcgcgta gcgcctgctg
    27241 ctttagcgtt cgttagtgaa aaaggtgaaa cctcggtcgg tgaactgcct tcgccgggta
    27301 gcctgtattt tcaacgtttt ggcggtttga atattgaaca ggaggcgtta tgccaattta
    27361 tcagatagac ggtctgactc cggttgtgcc agaagagagt tttgtccatc cgacagcggt
    27421 attgatcggc gatgttattc tcggcaaggg tgtttacgtt gggccaaatg ccagcctgcg
    27481 tggcgatttt ggtcgtatcg tggtgaaaga tggcgcgaac attcaggata attgcgttat
    27541 gcacggtttt cccgagcagg atactgtcgt tgaagaagat gggcatatgg gtcatagcgc
    27601 tatccttcac ggctgcatta tccgccgcaa tgcattagtg ggaatgaacg cggtagtgat
    27661 ggatggtgcg gtgattggcg agaacagcat tgttggcgca tccgcatttg tgaaagccaa
    27721 agcagaaatg cctgccaatt acctgattgt cggcagcccg gcgaaactga ttcgtgagct
    27781 tagtgagcag gagctggcat ggaaaaagca gggtacgcat gagtatcagg tgctggtgac
    27841 acgctgtaag cagacgttac atcaagtcga accattgcgg gaagttgaac ctggcaggaa
    27901 acgcctggta tttgatgaga atctgcgacc gaaacagtaa cgtccagata ctgaacgttc
    27961 taatttttag catgtgatac ctaaaccgca acgctgatac aggttgcggt ttttttattg
    28021 ccgaatgcgg cgctttacgc gttttgtttt ggtgtctttc aggacaatag agcaactcat
    28081 ccagtaatct tgtttacgcc ttcgcattat ttatctcttt tcgtttctat actgattttt
    28141 ctcaatccgt tttattacag ggcagggtgc gatgagcagc aatacattta ctctcggtac
    28201 aaaatccgtt aaccgtcttg gttatggcgc gatgcaactg gcaggtcctg gggtttttgg
    28261 tcccccaaga gatcgccacg tcgctataac cgtgctgcgc gaggcgctgg cattgggcgt
    28321 caatcacatt gataccagcg acttttatgg tccgcacgtc accaatcaga ttatccgcga
    28381 agcgctttat ccttactctg acgacctgac aattgtcact aaaattggtg cgcggcgtgg
    28441 agaggacgca tcctggttgc ccgcattttc tccggcagag ctgcaaaaag cggtgcacga
    28501 taatctacgt aatctcgggc tggacgtact ggatgtggtt aacttgcgca ttatgatggg
    28561 ggatggtcat ggcccagcgg aaggatcgat tgaagccagc ctgaccgtgc tggcagagat
    28621 gcaacaacaa ggcctggtaa aacatattgg cctaagcaac gtcacaccga cgcaggttgc
    28681 agaggcgcgc aagattgccg aaatcgtctg tgtgcagaac gaatacaaca ttgctcatcg
    28741 tgctgatgac gcaatgattg atgctttggc tcgtgacggc attgcctacg tgccgttctt
    28801 cccgctcggg ggctttacac cgctgcaatc ctctacgctg tcggatgttg ctgcgagcct
    28861 gggtgcaaca cccatgcagg tggcgctggc gtggctgtta caacgttcac cgaatatttt
    28921 gctgatccca gggacgtctt ctgttgcgca tttacgggag aatatggctg ctgaaaaatt
    28981 gcatctttct gaggaagtgt tgtctacgtt ggatggtatt tcgcgagaat aacgaatata
    29041 caaaagggaa agatgcattt cccttttgtt tcttttttaa tggcatggag tgcatatgtt
    29101 taaggccagg atttgcggct ggataggttt acttcccctg tttatgctga gtcttccagt
    29161 acaggcggaa cttcgatgtg ttgcaaatac agttgatatt gaaccatttt tttctgcggc
    29221 taccgctgaa gataaacaac aagttgaaca agctatcaac agtagcgtga atcttgtccc
    29281 cttcggttta tctgcatcgg actggaaagt gcatcgtggc gatttagtgg tagaaggtaa
    29341 tatagagagt aatcaaaaat tgattgttct tggtaatttg acagtcaaag gtaatatttc
    29401 cactttctct ctttctaatc catgggttat tctcggtaac gtgacggcaa cgaatatagt
    29461 tacggattca ccactattaa ttaccggttc gataaatgca agtggactgg tatttatcga
    29521 ctcatattac gataatccgt cttcgattaa ggggagtatt aatgcgcgtg gaatatttat
    29581 caatgacata attgcacctg ttgttgcgtc ttcgacaaat agtgaattca tggtccgtgc
    29641 gagtgacaaa aatgacactg aaaatgtcaa aaaagcgctg atgataataa atcctgatgc
    29701 atattattgg ggtctaataa atgatgaaga tgctctgaaa gaaattttta agcgaagcaa
    29761 tattcgcatg gcagggaatg tctgtaatca gatgaaaaaa gaagcgctgt ttcgccctaa
    29821 gccttctcct gagttagtgc aggaattgca aatgctggat gaaggcaacg ttgctgcatt
    29881 tgaaggacga gatattgcga catttgatct tgccataatg cgaactctcc caaggcttaa
    29941 aggaatttca gctaacctac gcaaacaact tattaatagc aatgatgaac aaacgattga
    30001 aagtatggcg agatatatgc ctgacaatga gattctggag ttgaccgatc agcaattggg
    30061 ttaccagcct gttgttctgg ggttgctcga ccgtgaaccg ctctctgtcg aaataatgac
    30121 gcgaatgagt cgtctacctg atggtgtcgg tccgttgaat cttgcgctac gtgaaaatct
    30181 ccctctggat atcgtaatga ctctggcgaa aagagattgg gatatgatca ttcaggagct
    30241 ttataaagat gcgtggttat tgcctgaatc cattattgat ggctatatcc gcagtgatga
    30301 ttcctctatt cgtcaggtcg gtgctggagg acaactcacc tacaatcagg caatgcagtt
    30361 ggcgaacgat tcatcaaaca atgttgtcac aagcttagcg ttcaagctgg cagagatgaa
    30421 acaccatggt caattattgc ggatgacgcc acaagagagt gacaaagttg cagcctattt
    30481 ataccaaaaa ttcgagaatg acgatgatct aatacgtgtg ttatttttag cattgccaga
    30541 taacttacag tttaattttg ttaaaaggat ggagaaaaaa tcccctgcct acttttgctg
    30601 tcgggatatg caggtaattc actctgacgc tgctctacaa cggttattga cacgtttcaa
    30661 cgatcctgag gggtggagta atctggcgaa aaatcagtat ctgagcacgt cgatgaaaca
    30721 aaaaatttgg caacgtgctt tatcgcatcg gaaaaataat ccgaaagcag attcagctgc
    30781 gtatgaaacc agcgctgata tgattttgtc tgagcttatc agccacggtg aagttgatga
    30841 tcagatgcta ttaaatgcca ccgccctgat acgtttggaa gactgggact ttttagagag
    30901 tgcattagtt agttgggata atttacccgc tgttgtcctc aaggaattac agcaaaatac
    30961 gccacgcaat gatatttggg cgaagttttt tctgaggcag gaaaacagct cccgtgcgca
    31021 ggttgatgaa gcattacgcg tctattatgc actcgacccc gatgcgttag cacaactgga
    31081 tgtactggca aaacaaccgg atcgtatatg gtggagtaca ctggcgaaaa gcaatctcac
    31141 atttttcaag ttcggcgcac ttaacaaccg ccacacaccg cctgcagtac tggcggcaga
    31201 aattgatccc gagtggtgga ttgtggcgat gaataatccc cgttttccag ttgatgtatt
    31261 aaaggcgagg ctgaaacgtg atcctttgct ggcgttagag cttgttaacc ctgaactgga
    31321 tttagtccgc cagctggcgc ttaacggtaa gacgcgcgca atacgggaac aagcgatgag
    31381 aaaacttgat gagttgtatt gatccacaac gtaatatttc ttaacccttt gattaattgg
    31441 ggataattat tcctgacagt gagtccccaa taccttgata tattctgaat ttttaatgaa
    31501 gcggcgtgtt gcgatatctc cgtcagggga attgatgcac catagcgcaa accgaattat
    31561 caaggattga taatgacgct ctaccagata aaaccgctct ttcagtcgct gttaaggccg
    31621 acgatgtttt ggctttacaa gcaccacgtt acagcgaatc acatcactct tgctgcactg
    31681 gcgctttctc ttcttaccgg attgctgttg atgttggtgg cacaacccat cctctttatg
    31741 ctattgccca tcgtgctttt tatccgtatg gcgctcaatg cgctggatgg catgttggcg
    31801 cgtgagtgca atcagcaaac acggcttggg gcaattttga atgagactgg tgatgttatt
    31861 tccgatattg cgctctattt accattttta tttttaccgg aaagtaacgc atcactcgtg
    31921 atactcatgt tattttgcac catattgacc gagttttgcg gtttactcgc gcagacgatt
    31981 aacggtgttc gcagttatgc cggtccattt ggcaaaagcg atcgcgcgtt aatatttggt
    32041 ctgtggggtc tggccgttgc catttatcca cagtggatgc agtggaataa tcttttatgg
    32101 agcattgctt caattctgct tctctggact gcgattaatc gttgtcggag tgtgcttctt
    32161 atgagcgctg aaagataatg ctggaaaaat ctctggcaac acttttcgcg ttgttaattt
    32221 tagcaacgct gataaaccat tttctggtat ggcggttacc ggagagaaaa gaggatgaag
    32281 ttacattacg tattcgtacc tggtggggca tcgtcatttg tttttcactg gtgatttcag
    32341 gtccacgctg gatgacgttg acgttttttg cgctgataag ttttctggca ttgaaagaat
    32401 attgtacgct tatatctgta cattttccgc ggtggttata ttgggttatt cctcttaact
    32461 atttgtttat cggttttaat tgctttgagc tgtatctatt atttatacct ttggctgggt
    32521 ttctaatatt agccaccggg caagtttttg tgggggaccc ctccggtttt ctgcataccg
    32581 tgagcgccat tttttggggc tggataatga ccgttttcgc cttgagtcat gccgcctggt
    32641 tattaatgtt gccaaccaca aatatccagg gcggggcgtt actggtgtta tttcttttgg
    32701 cattaaccga gtcaaacgat attgcacagt atttatgggg aaaatcctgc ggcagaagaa
    32761 aagtggtccc caaagtcagc ccgggaaaaa cgctggaagg tctggtgggg ggcgtcatca
    32821 ccaccatgat tgcgtcactg attattggac cgttgctgac accgctaaat acattacagg
    32881 cattattagc gggtttgtta attggtatta gtggtttttg cggcgatgtc gtgatgtcag
    32941 ccatcaaacg agatattggt gttaaagata gtggaaaact attaccagga catggcggac
    33001 tccttgacag gattgactca ttaattttca ccgctccggt atttttttat tttattcgct
    33061 acagctgtta ctgaaggaaa atagagaaaa tggaaaattc acgcatccct ggggaacatt
    33121 tttttaccac cagtgataat acagcgttgt tttatcggca ctggcccgct ttacagcccg
    33181 gggcgaaaaa ggtcatcgtc ttatttcatc ggggtcatga acattctggt cgtctacaac
    33241 atctcgttga tgaactggcg atgccagata ctgcttttta tgcatgggat gcccgggggc
    33301 atggaaaaac ttcggggccg cgtggttata gcccatctct tgcgcgttca gtgcaggatg
    33361 tcgatgaatt tgtccgtttt gctgccagcg acagccaggt tggactggaa gaggtggttg
    33421 tgatcgcgca aagcgtcggc gcagtgctgg ttgccacatg ggttcatgat tatgcgcctg
    33481 caattcgcgg gctggtgctg gcttctccgg cctttaaggt taaattgtat gtgccgctgg
    33541 cacgtcctgc gctggcgtta tggcatcgtc tgcgtggtct gttttttatt aattcctatg
    33601 tgaaaggacg ctatttgacc cacgatcggc aacgggtggc gagtttcaat aatgatccgc
    33661 tgatcacacg ggcgattgcc gtcaatatct tgctcgatct ctacaaaacg tctgaacgta
    33721 ttattagaga tgcggcggcg attacgctcc ccacgcaact tctgatatca ggcgatgact
    33781 atgtggtgca tcgccaaccg cagattgatt tttatcagag attacgtagc cctctgaaag
    33841 agctgcatct gctgccaggc ttttatcacg acacgttggg tgaagagaac agggcgcagg
    33901 catttgaaaa aatgcaaagc tttattagtc gtttatatgc taacaagtca caaaaatttg
    33961 attatcagca tgaagaccgc actggaccat cagcggatcg ctggcggctc ctttcaggtg
    34021 gacccgtgcc attatcgccg gttgatttgg cgtatcgctt tatgcgaaag gcgatgaaat
    34081 tgttcggggc gcactctgcg ggcctgcatc tcggaatgag caccggcttt gattcaggca
    34141 gttccctgga ttatgtctat caaaatcaac cgcaaggcag taacgcattc gggcgcttaa
    34201 ttgacaaaat ctacctgaac agtgttggct ggcgcggtat tcgccagcgt aaaacccatt
    34261 tacaaatact gattaaacaa gccgttgccg atctccacgc caaaggttta gccgtccgcg
    34321 tggttgacat tgccgcaggg catgggcgct atgtactgga tgcgctggca aacgagcctg
    34381 ccgtaagcga tattttgtta cgtgattaca gtgagttaaa tgttgcacag gggcaagcga
    34441 tgattgccca acggggaatg tctgggcggg tgcgttttga acagggcgat gcgtttaacc
    34501 cggaggaact cagcgcgtta actccgcggc ctacgctggc gattgtctct ggcctgtatg
    34561 agctttttcc cgaaaatgag caggtaaaaa actcactcgc aggtcttgcc aatgccatcg
    34621 aaccgggcgg cattctcatc tacaccgggc agccgtggca ccctcaactg gagatgattg
    34681 ccggggtgtt aaccagtcat aaagatggta aaccgtgggt aatgcgcgtg cgttcgcaag
    34741 gggagatgga ttcactcgtg cgtgatgccg gatttgataa atgcacacaa cggattgatg
    34801 agtggggtat ttttacggtt tcgatggcgg tgcgtcgtga taactgaacg tcggaacgta
    34861 ttgctacaag gcgctggctg gttattgttg ctggccccgt ttttcttctt cacctatgga
    34921 tctcttaatc agttcaccgc ggttcaggac cttaacagcc atgatatccc cagtcaggta
    34981 ttcggttggg aaacggcgat cccttttctt ccctggacta ttgttcctta ctggagtctg
    35041 gatcttttat atggattttc gctgttcgtt tgtagcacga cattcgaaca gcgccgactt
    35101 gtccaccggc ttattctggc aacggtaatg gcctgctgcg gttttttgct ctatccgctg
    35161 aagtttagtt ttatccgtcc tgaagtgagt ggggtgacgg gatggctatt ttcgcaactt
    35221 gaactgtttg atctgcctta taaccagtct ccttcgctgc atattattct ctgctggcta
    35281 ctttggcgtc actttcgtca gcatctggct gagaggtggc gtaaagtctg cggcggatgg
    35341 tttttactca tcgctatttc tactctgacg acctggcagc atcattttat tgatgtcatc
    35401 acagggctgg cggtaggtat gttgattgac tggatggtgc ccgtcgaccg tcgttggaat
    35461 tatcagaaac ctgatcaacg tcgaatcaaa atagcactgc cctatgtcgt aggcgcgggc
    35521 tcgtgcattg tattgatgga gctaatgatg atgattcagt tatggtggtc agtctggtta
    35581 tgttggccag tattatcgct actcataatt ggccgtgggt acggtgggct tggcgcgata
    35641 acaacaggga aagatagtca ggggaaacta ccgcccgccg tttactggct gacattgccc
    35701 tgccgcatcg ggatgtggct gtctatgcgt tggttttgtc gtcgcctgga gccggtgagc
    35761 aaaatgactg ctggtgttta tttaggggcg tttccacgac atattccggc acagaatgcg
    35821 gttctggacg tcacctttga attccctcgc ggacgagcga caaaagatcg actctatttc
    35881 tgtgtaccga tgctggatct ggtggttccg gaagaggggg agctccgaca ggccgtggcg
    35941 atgctggaaa cattacgcga agagcaaggc agcgttctgg tccattgcgc gttgggatta
    36001 tcgcgcagtg cgctggtagt ggcggcatgg ttgttatgtt acggacactg taaaaccgtt
    36061 gatgaagcga ttagttatat tcgagccaga cgttcgcata ttgtgcttaa ggaagatcac
    36121 aaagcgatgc tgaaattatg ggaaaacagg taagtggatt gagatgtgga ctgaactaca
    36181 gtccacatca agatcgtgtc cggttatgca gcaacaatgc tgtcgatggc cgctttcgcg
    36241 tcagactgcg ctttcgctgc catttccgga ccgtatgcga tcccttcggc gaagacaaat
    36301 ttcacatcgg taatgccgat aaagccgagg aacgtggaca gatacggcgt caccaggtcc
    36361 gttggtccat ctttgtggat cccaccgcgg ctggtaataa cgatggcttt tttacccgtt
    36421 accagacctt ccggaccgtt ctcggtatag cggaaagtaa cgcctgcgcg tgcaaccagg
    36481 tcaaaatagt ttttcaactg agttgagatg ttgaagttat acatcggtgc cgcaataacg
    36541 ataacgtcgt gggctttcag ctcggcaatc agctcatcgg aaagcgccag agcttcctgc
    36601 tgacgcggag tcagcggcgc atcgctcgga cgcagtgcac caaccagttc gccatccagt
    36661 accggaatcg gatttgcagc caggtcgcga acggtgattt catcagcgga gtgcttttcg
    36721 cgccattgtt caacaaaata atcggacaac tgattagact gagagtaccc tgccaggatg
    36781 ctggatttaa gaactaatac cttgctcatg gtgtttcctt atagatgttt gaatgggcga
    36841 tgccccgttg cttgctgaca ctttattcac aatcctgcca cagagatagc gcaataaatc
    36901 gaagcctatg ttcgaattta ttgaacaacg catagaaagc cgcgatgtgg tactctatat
    36961 ctatcattta aaagaaaatt aatcaggcag actactgccc actaacgtta tgacagaaca
    37021 acaaaaattg acctttacgg ccttgcagca gcggctggat tcgctgatgc tgcgcgacag
    37081 actgcgtttt tctcggcgtc tgcacggtgt taagaaggtt aaaaatcctg atgcacaaca
    37141 ggccattttc caggagatgg cgaaagagat tgaccaggcg gcagggaaag tcctgctgcg
    37201 tgaagcggca cgaccggaaa ttacttatcc tgacaattta ccggttagtc agaaaaaaca
    37261 ggacattctc gaagcgattc gtgatcacca ggtggtgatc gtcgccgggg aaacgggttc
    37321 tggtaaaacg actcagttac cgaaaatctg tatggagctg gggcgcggga ttaaagggct
    37381 gatcggccat acccagccgc gtcgcctggc ggcaagaacg gtggcgaacc gtattgcgga
    37441 agagctgaaa acggagccgg gcggttgcat cggttacaaa gtgcgtttca gcgatcacgt
    37501 tagtgataac acgatggtca agctgatgac cgacggtatc ctgctggcgg agatccagca
    37561 agaccgcctg ctgatgcagt acgacaccat cattattgac gaagcacacg aacgcagcct
    37621 gaatatcgat tttctgctcg gctatttgaa agagttgctg ccgcggcgtc ctgacctaaa
    37681 aatcattatc acttccgcga ctatcgaccc ggaacgcttt tcgcgccact ttaataatgc
    37741 gccgattatc gaagtctccg gtcggaccta tccggtggaa gtgcgctatc gaccgattgt
    37801 tgaagaagcc gacgacaccg agcgcgatca gttgcaggcg atttttgacg ccgtagacga
    37861 actgagtcag gaaagccctg gcgacattct gatctttatg agtggtgagc gggaaattcg
    37921 cgataccgcc gatgcgctga acaagctaaa tctgcgtcat accgaaatct tgccgcttta
    37981 tgcgcggctt tcgaacagcg aacagaaccg cgtgttccag tcgcacagcg gacggcgcat
    38041 tgtgctggcg accaacgtcg cggaaacctc gctgaccgta ccggggatta aatacgttat
    38101 cgaccccggt acagcacgta tcagccgcta cagctatcgc accaaagtgc agcgtttgcc
    38161 gattgaaccc atttcgcagg cgtctgccaa tcagcgtaaa ggccgctgtg ggcgtgtgtc
    38221 cgaagggatc tgtattcgtc tctattccga agacgatttc ctttcgcgcc cggagtttac
    38281 cgatccggag attctgcgta ccaacctggc ctcggttatt ttgcagatga ccgcgctggg
    38341 gctgggcgat atcgctgcgt tcccgtttgt cgaagcaccg gataaacgca atatccagga
    38401 tggcgtgcgt ctgctcgaag aactgggcgc gatcaccacg gatgaacagg ccagcgccta
    38461 taaactgacg ccgctcggtc gccagctctc gcagttgcct gtcgatccac gtctggcgcg
    38521 tatggtgctg gaagcgcaaa aacatggctg cgtgcgtgag gcgatgatta tcacgtccgc
    38581 gctttccatt caggatccgc gcgagcgtcc gatggataaa cagcaggcat cggacgaaaa
    38641 acatcgtcgc ttccacgaca aagagtctga cttcctcgcg tttgtgaatc tgtggaatta
    38701 tcttggcgag cagcaaaagg cgctttcttc caacgccttc cgtcgcctgt gtcgtaccga
    38761 ttatctcaac tatctgcgcg tgcgcgaatg gcaggatatc tacacccagt tgcgccaggt
    38821 ggtgaaagaa cttggcattc cggttaacag cgaaccggcg gagtatcgcg aaattcacat
    38881 tgcgttgctg acgggtttac tttcccatat cggcatgaaa gatgccgata aacaagaata
    38941 taccggcgca cgtaacgcgc gtttctccat ctttcccggt tctggtttat tcaaaaaacc
    39001 gccgaaatgg gtaatggtgg cggaactggt agaaaccagc cgcctgtggg ggcgcattgc
    39061 tgcgcgtatc gacccggaat gggtggagcc tgtcgctcag catttgatta aacgcaccta
    39121 cagcgaacct cactgggaac gggcgcaggg cgcggtgatg gcaacggaaa aagtcactgt
    39181 ttatggtttg ccgattgttg ccgcgcgcaa ggtcaactac agccagatcg atccggcgtt
    39241 atgccgtgaa ctctttattc gccatgcgct ggtggaaggt gactggcaga cgcgtcacgc
    39301 attcttccgt gaaaacctga aactgcgggc cgaagtggaa gagctggaac acaaatcacg
    39361 tcgccgcgat attctggttg atgacgaaac gttgtttgag ttctatgacc agcgcatcag
    39421 ccacgatgtg atctctgcac gccacttcga cagctggtgg aaaaaagtca gccgcgaaac
    39481 ccctgatttg ctcaactttg aaaaaagcat gttgatcaaa gagggcgcag aaaaaatcag
    39541 caagctggat tacccgaact tctggcatca gggcaatctc aagctgcgtt tgagctatca
    39601 gtttgagccc ggcgcggatg ctgacggtgt gaccgtacat attccgctgc cgttacttaa
    39661 ccaggttgag gaaagcgggt ttgaatggca gatccccggt ctgcgccgcg aactggtgat
    39721 tgctctgatt aaatcgttgc cgaaaccggt acgccgtaat tttgtacccg caccaaacta
    39781 cgccgaagcg tttttaggcc gcgtcacacc gctggagtta ccgttgctcg acagccttga
    39841 gcgcgagtta cggcggatga ccggtgttac cgttgaccgc gaagactggc actgggatca
    39901 ggtgcccgat cacctgaaaa tcaccttccg cgtggtggac gacaaaaata agaagctaaa
    39961 agaagggcgc tcattacagg atctgaaaga tgcgctgaaa ggcaaagtgc aggaaacgct
    40021 gtccgcggtg gcggatgacg gtatcgagca gagcgggctg catatctgga gctttgggca
    40081 actgccggaa agctacgaac agaagcgcgg caactataaa gtgaaggcgt ggccggcgct
    40141 ggtggatgaa cgtgacagtg tggcgatcaa actgtttgat aacccgctgg agcaaaaaca
    40201 ggcaatgtgg aacggtcttc gccgtctact gctgctaaat attccctcgc cgatcaagta
    40261 tttacatgag aagttaccaa acaaagccaa gcttggactg tactttaacc cgtatggcaa
    40321 agtgctggag ctgatcgacg actgtatctc ctgcggtgtg gatcaactga tcgacgccaa
    40381 tggtggcccg gtctggacgg aagaaggctt tgctgcgctg catgagaaag tgcgtgccga
    40441 actgaacgac acggtggtag atattgcgaa gcaggtcgag caaattctta cggcagtgtt
    40501 caatatcaac aaacgtctga aagggcgggt ggatatgacc atggcactgg ggctttctga
    40561 cattaaagcg cagatgggcg ggttggtata tcgcggtttt gtcactggta acggcttcaa
    40621 acggctgggc gacacgctgc gatatttgca ggcgattgaa aaacggctgg aaaaactggc
    40681 ggttgatcca catcgcgacc gtgctcagat gctgaaagtt gaaaacgtcc agcaggcgtg
    40741 gcagcaatgg atcaacaaac tgccgcctgc acgtcgtgag gatgaagacg tgaaagagat
    40801 ccgttggatg atagaagagt tgcgcgttag ttacttcgct caacaacttg gtacgcctta
    40861 tccgatttca gataagcgta ttttgcaggc gatggagcag attagcggtt aaccctgcta
    40921 tttgcctgat aaagaaaaaa cccggtaagc atttagcgcc gggtttttta ttaattctaa
    40981 aacggtaagg gtaaaaattc aggaattcag aaaaatacaa ttctctgctg caagatgaat
    41041 aatgtttatc tacagcattt ccttaaaaga tatgtcaggc ttgcggagtg gcggttaagg
    41101 acatacgact tcctcctttc agagtgctcc gcttctcact attatctcac acagtattct
    41161 taagggaacg ataaggagga accatgaaca ttaccccgtt tccgacgctt tcgccggcaa
    41221 ctatagatgc cataaatgtt atcggacagt ggctggcgca ggatgatttc tccggtgagg
    41281 tgccgtatca ggccgattgc gtgatccttg caggcaatgc ggttatgccg actatcgacg
    41341 cggcatgtaa gattgctcgc gatcagcaaa ttcctttact gattagtggt ggtatcggtc
    41401 actcgacaac ttttttgtat agcgccatcg cacagcatcc gcactacaac actatccgca
    41461 ccactggcag agcagaagcg accatcctgg cggatatcgc tcatcagttc tggcacattc
    41521 cgcatgaaaa aatctggatt gaagaccagt caacaaactg cggtgaaaac gcacgcttta
    41581 gcatcgcgct attgaaacag gccgtagaac gagttcatac ggctatcgtt gttcaggacc
    41641 ccaccatgca gcggcgcacg atggcgacgt tccgccgtat gactggggac aatcccgatg
    41701 caccacgctg gttaagttat cccggattcg ttcctcagtt aggaaataac gcagacagtg
    41761 taatctttat taatcagtta caaggattat ggccggttga gcgttatctc tcactactca
    41821 ctggcgagct gccgcgttta cgcgatgata gcgatggcta cggtccccgc gggcgagatt
    41881 ttatcgttca cgttgatttt ccggcagaag tcatccatgc atggcaaacg ctgaaacatg
    41941 atgcggtgct catcgaggcg atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg
    42001 aaccacttgt ttgcaaacgg gcatgactcc tgacttttat ttctgccttt tattcctttt
    42061 acacttgttt ttatgaagcc cttcacagaa ttgtcctttc acgatgccgt ctctctgatg
    42121 attgatgtta attaacaatg cattcaccga aaataaacat ataaatcaca ggagtcgccc
    42181 atgtcagtac ccgttcaaca tcctatgtat atcgatggac agtttgttac ctggcgtgga
    42241 gacgcatgga ttgatgtggt aaaccctgct acagaggctg tcatttcccg catacccgat
    42301 ggtcaggccg aggatgcccg taaggcaatc gatgcagcag aacgtgcaca accagaatgg
    42361 gaagcgttgc ctgctattga acgcgccagt tggttgcgca aaatctccgc cgggatccgc
    42421 gaacgcgcca gtgaaatcag tgcgctgatt gttgaagaag ggggcaagat ccagcagctg
    42481 gctgaagtcg aagtggcttt tactgccgac tatatcgatt acatggcgga gtgggcacgg
    42541 cgttacgagg gcgagattat tcaaagcgat cgtccaggag aaaatattct tttgtttaaa
    42601 cgtgcgcttg gtgtgactac cggcattctg ccgtggaact tcccgttctt cctcattgcc
    42661 cgcaaaatgg ctcccgctct tttgaccggt aataccatcg tcattaaacc tagtgaattt
    42721 acgccaaaca atgcgattgc attcgccaaa atcgtcgatg aaataggcct tccgcgcggc
    42781 gtgtttaacc ttgtactggg gcgtggtgaa accgttgggc aagaactggc gggtaaccca
    42841 aaggtcgcaa tggtcagtat gacaggcagc gtctctgcag gtgagaagat catggcgact
    42901 gcggcgaaaa acatcaccaa agtgtgtctg gaattggggg gtaaagcacc agctatcgta
    42961 atggacgatg ccgatcttga actggcagtc aaagccatcg ttgattcacg cgtcattaat
    43021 agtgggcaag tgtgtaactg tgcagaacgt gtttatgtac agaaaggcat ttatgatcag
    43081 ttcgtcaatc ggctgggtga agcgatgcag gcggttcaat ttggtaaccc cgctgaacgc
    43141 aacgacattg cgatggggcc gttgattaac gccgcggcgc tggaaagggt tgagcaaaaa
    43201 gtggcgcgcg cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg
    43261 aaaggatatt attatccgcc gacattgctg ctggatgttc gccaggaaat gtcgattatg
    43321 catgaggaaa cctttggccc ggttctgccg gtggtcgcat ttgacacgct ggaagaggct
    43381 atctcaatgg ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg
    43441 aacgtcgcga tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa
    43501 aacttcgaag ctatgcaagg cttccacgcc ggatggcgta aatccggtat tggcggcgca
    43561 gatggtaaac atggcctgca tgaatatctg cagacccagg tggtttattt acagtcgtaa
    43621 tgagtaaaag aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt
    43681 tcgagggtgc gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt
    43741 ttcaccagtt gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa
    43801 ccgaaatggc tgccaatgat atcggaagag actatttctt catcggtata accaaatgac
    43861 tcgttattgg tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttt
    43921 ccgagaatcg ataccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
    43981 cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcccccgta
    44041 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgtggg
    44101 ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct
    44161 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa gacagttagt ggtgcatgac
    44221 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattgacgtt ataaacgata
    44281 gttttcattt caccggcagg ggcggaaatc aacaccttct tcgcaccagc atcaagatgc
    44341 gcctgcgatt tctcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcccct
    44401 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
    44461 tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaacggacc atagtttgaa
    44521 tcatgtttca gcagataggc gagaattttt ggggaagtga gatcattaat agcgacaacg
    44581 tctatgttgc ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa
    44641 ccgttaatac caactttact catggttttc tcctgtcagg aacgttcgga tgaaaattga
    44701 tcctttccag gcttagccca ggattgtggg atgggcaatc tccattctta cagtgaaacg
    44761 taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt
    44821 aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat
    44881 cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg
    44941 tggtttcttc ccacgtagcg atcggccact catcaacatg gttcatgttt cctgtggtat
    45001 ctcaattctc gtgctgatgg tggttcgtct tttgttaaga ctgaaatacc caaccccgcc
    45061 gattatacct aagccaaaac cgatgatgac gggactggcg catttgggac atttggtgat
    45121 ttatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa
    45181 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt
    45241 tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg tcatcgggtt
    45301 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
    45361 gatgccgcgt aaacgttcct gaaggatatt taaagaaaac gcctgtacta aaaccgaccc
    45421 gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga
    45481 caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc
    45541 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat
    45601 aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa
    45661 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
    45721 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcctcga
    45781 aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc
    45841 agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
    45901 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
    45961 tgaatgtcag aatcgtaatg cagataatga gcagacacac caccagaggg ttgtgcttca
    46021 tagcctttct ccttgccgga tggcgggtaa gaggctaaga tctgaattgc taggttcatt
    46081 cgttggcctc ggttgataga aatatcggtc ggggccttcg tctttctgat tcccggttag
    46141 cctgaaaaca gaaagtctca ggcacccgca ggcatcctat gaggtttcct tagggacgaa
    46201 aataatcact tcacgaaatt gcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc
    46261 agtttagcca ttcgttactc tctttattcc aatagcatta attttctctg caataattgt
    46321 tgtaaaaatg tgacgcaaag aggtttttgg tcataagtaa ttaccgtcaa gtgccgatga
    46381 ctttctatca ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacat
    46441 cgattaggtt ttttgcatca catcaggctg gttccgttat ttgcctgcat tcttggcggt
    46501 atcttagttc tattcgcatt aagttctgct ctggctggct atttcctctg gcaggccgat
    46561 cgcgatcagc gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca
    46621 gattttttgc gttcagcccg gatcaatatg attcaggccg gggctgcgag tcgtattgcg
    46681 gaaatggaag caatgaagcg caatattgcg caagccgaat cggagattaa acagtcgcag
    46741 caaggttatc gtgcttatca gaatcgaccg gtgaaaacac ctgctgatga agccctcgac
    46801 actgaattaa atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat
    46861 gccaaaaatg gcatgtttga agcgattatt aatcatgaaa gtgagcagat ccgaccgctg
    46921 gataatgctt ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagggccaac
    46981 caactggcgg aactggccca tcagcgcacc cgcctgggtg ggatgttcat gattggcgcg
    47041 tttgtgcttg ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt
    47101 cgtccactgc aacatgccgc acaacggatt gaaaaaatcg ctagtggcga tctgacgatg
    47161 aaggatgaac cggcgggtcg taatgaaatc ggtcgcttaa atcgtcattt acagcaaatg
    47221 cagcattcac tggggatgac agtagggact gttcgacagg gtgcggaaga gatttatcgt
    47281 ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg
    47341 gcggcaatcg aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat
    47401 gcggataacg cgcatcatgc cagcaaactg gcgcaagagg cttctattaa agccagcgat
    47461 ggcgggcaga cggtttccgg tgtagtaaaa acgatgggcg ctatctccac gagttcgaag
    47521 aaaatttctg agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca
    47581 ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc
    47641 gccagcgaag tacggacact cgcaagccgc agcgctcagg cggcgaaaga gattgaaggc
    47701 ttgatcagtg aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggcaggg
    47761 aaaaccatga gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc
    47821 gccgccgcct cggatgaaca aagtagaggc ataactcagg ttagccaggc gatttctgaa
    47881 atggataagg tgacgcaaca gaatgcttct ctggtagaag aggcctcagc agcggcggtg
    47941 tcccttgaag aacaggctgc acgattaact gaggcggtgg atgtattccg tctgcacaaa
    48001 cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag ttagtttcgc tacggtgtga
    48061 aaatgttcaa ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa
    48121 tagcgttaat catcaactgg caaccagaag agaacgtcgc atctacgcgg gtcagtattc
    48181 caatcggttc gcctgcacca tgtcccggaa caggcagggc caccagcgtg gcatgacgca
    48241 ggtcgtcttt tacagcgcca gaagggacaa accacacgta atcgtattca accgtaagtt
    48301 gacgagatag cgaagcagac agcgtttcga tacaacccga aggaatttta cagccctggc
    48361 tctgcactaa tgcatctgaa tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg
    48421 gccattccag cacccggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga
    48481 caaccagctt caacgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag
    48541 gatctgacat ccgaccaatg ccgatatcga tttccccggt ttttaaaccc gccagaatca
    48601 tagggttact cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac
    48661 ctataaccga aggtaatatc cccagtgccg cagtaggtag tgcaccaacc ctgacgacat
    48721 cattattaag accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa
    48781 ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac
    48841 cacgctcaaa caagcgagcg ccagtcagct gctccagttc attcaatgtc ttagagagcg
    48901 caggttgact caaattaagg gtttcagccg cgcgccccaa agttccttgt tgtgcgacag
    48961 ctacgaatgt atgaaggtgg cgcaaacgga tgcgctgact aaacagacta tttttttcca
    49021 taagcgatgt taaaaacgaa gcggtgtcgc tgacaagtga agttgtttga ttatgataac
    49081 ttgattgcaa aatattatta acaattaaag caattatgtt acagcaaaat ggataatatt
    49141 gatgttttcg cggcgagatc acagtttgta aattcttccc gcaagagtga atgcggttac
    49201 ctacactcca gattactgac cactggaggc agacactatg gcgaacagca tcacggcgga
    49261 tgagattcgg gaacagtttt cgcaggcaat gtcagccatg taccagcaag aagttccgca
    49321 atatggcacg ctgctggaac tggtagctga tgtgaatctg gctgtgctgg aaaacaatcc
    49381 tcaactgcac gaaaaaatgg taaatgcaga cgagctggcg cgactgaatg ttgaacgtca
    49441 tggggcgatt cgcgttggga ctgcacaaga gcttgctact cttcggcgga tgtttgccat
    49501 tatggggatg tacccggtga gctattacga tctctcgcag gcaggggtgc cggtacattc
    49561 gacagcattt cggcccattg atgatgcttc tctggcgcgt aatcccttcc gcgtttttac
    49621 ctccttactc cgccttgagc ttatcgagaa cgaaattttg cgccagaaag cggcggagat
    49681 tctgcgtcag cgcgatatct tcaccccacg ttgtcgacaa ctgttagagg aatatgagca
    49741 gcagggcggt tttaacgaaa cacaggcaca ggagtttgtg caggaagccc tggaaacgtt
    49801 tcgctggcac cagtcagcaa cggtagatga agaaacctat cgcgccttgc acaacgaaca
    49861 tcggttgatt gctgatgtgg tctgttttcc tggatgccat atcaaccacc tgacaccacg
    49921 tacgctggat attgaccggg tgcagtcgat gatgcctgaa tgcggaattg aacccaaaat
    49981 tctcatcgag gggccgccgc gccgcgaggt accgatttta ctacgccaga ccagctttaa
    50041 agcactggaa gagacggtgt tgtttgcggg gcagaaacag ggcacgcata ccgcgcgctt
    50101 tggtgaaatt gagcagcgtg gcgtggcatt aacgccgaaa gggcgacaac tgtatgatga
    50161 tcttctgcgt aacgctggaa ccgggcagga taatctcact caccaaatgc atttacagga
    50221 aaccttccgc acttttcctg acagtgagtt tttaatgcgt cagcaagggc tggcatggtt
    50281 ccggtaccgt ctgacgcctt caggtgaggc gcatcgtcag gcgattcatc ccggagacga
    50341 tccacagccc ttaattgaac gtggttgggt cgcggcgcaa cccattacct atgaagattt
    50401 cttgcccgtt agcgcggcgg ggatcttcca gtcaaatctg ggtaatgaaa cgcagacacg
    50461 cagtcacggt aatgccagtc gcgaagcatt tgagcaggcg ttgggttgtc cggttttgga
    50521 tgagttccag ctttaccagg aagcggaaga acgcagtaaa cgtcgctgtg gtttgcttta
    50581 aattctgacc atccgccttt gcaaaaattt gcctgatttt acaaacgaat caggctcatc
    50641 ccatcgacat aaaaaaaatg ccgatttatg catattctct cagttcaaca attggattat
    50701 taataaatat tgtctagagt gagcggtcat aaataagcac tttcttgccg ctgaaaacga
    50761 ccagcgcggg accattcaca acaccagaag gactcacttt caggtatgga tcgtagacga
    50821 tttattaaag gttcaatggc tatggccgcc gtgtgcggta ccagcggcat tgcttctctt
    50881 ttttctcagg cggcattcgc ggcagattct gatattgccg acgggcaaac ccagcgtttt
    50941 gacttctcca ttctacagtc aatggcgcac gacttagcgc aaacagcgtg gcgtggtgcg
    51001 cctcgtccgt tacctgacac gctggcgaca atgacgccgc aggcttataa cagtattcaa
    51061 tacgacgccg aaaaatcgct ctggcataac gttgagaacc gtcaactgga cgctcagttc
    51121 ttccatatgg gaatgggatt ccgtcgccgc gttcgtatgt tttctgtaga tccagcaaca
    51181 catctggcgc gtgaaattca ctttcgcccg gagttgttca aatacaacga tgcgggtgtt
    51241 gatacaaaac aattagaagg gcaaagcgat ctcggctttg ccggttttcg cgtgtttaaa
    51301 gcccccgaac tggcgcgccg tgatgtagta tcatttctcg gcgcgagtta tttccgcgcc
    51361 gttgatgata catatcaata cggtttgtcg gcccgcggcc tggcgatcga cacttacact
    51421 gacagtaaag aagagttccc cgactttacc gccttctggt ttgatacggt aaaaccgggg
    51481 gcaactacct ttaccgttta tgcgttgctc gatagcgcca gcattactgg tgcctataag
    51541 ttcactatcc attgtgagaa aagtcaggtg attatggatg tggaaaatca cctgtatgcg
    51601 cgcaaagaca ttaaacagct gggcattgcg ccgatgacca gtatgttcag ctgcggtact
    51661 aatgaacgtc ggatgtgcga tacaattcat ccgcaaattc atgactctga tcgtctgtcc
    51721 atgtggcggg gcaacggcga gtggatttgc cgtccgctga ataatccgca aaaattgcag
    51781 ttcaatgctt acaccgacaa caacccgaaa gggtttggtt tattgcaact ggatcgtgac
    51841 ttctcccatt atcaggacat tatgggctgg tataacaaac gcccaagtct gtgggtggag
    51901 ccgcgtaaca agtggggtaa gggcaccatc ggcctgatgg aaatcccaac aacgggcgaa
    51961 acgctggata acattgtctg cttctggcag ccagaaaaag ctgtaaaagc aggtgatgag
    52021 tttgcattcc agtatcgtct gtactggagt gcgcaaccgc ctgttcattg cccattagcg
    52081 cgcgttatgg cgacgcgtac cggcatgggc ggtttcccgg aaggttgggc accaggtgaa
    52141 cactatcccg aaaaatgggc gcgtcgtttt gccgtcgatt tcgttggtgg tgatctgaaa
    52201 gctgccgcac caaaaggcat tgagccggtg attacgcttt ccagtgggga agcgaagcaa
    52261 atcgaaattc tctatattga acccattgat ggttatcgta ttcagtttga ctggtatccg
    52321 acttcggact ccactgatcc ggtcgatatg cggatgtatc tgcgttgtca gggcgacgct
    52381 atcagtgaaa catggctgta tcagtatttc ccgccagcgc cggataaacg tcagtatgtt
    52441 gacgaccgcg tgatgagtta atcgtttttt cttcggcacc ttcttcggga ggtgccgtct
    52501 ggttaaacac gatcccgctc gcatttttcc ctaagttaaa tgagtaatct gatggtgtgt
    52561 atttcagata caccttgtca gccactaaca gggagtgcgt atgtttccag aataccgaga
    52621 tttaatatcc cgtctgaaaa acgaaaatcc tcgctttatg tccttgttcg ataaacacaa
    52681 taaacttgat catgaaattg ccagaaagga aggttccgac ggtcgagggt acaatgcgga
    52741 agtggtccgc atgaaaaaac aaaagctaca gttaaaagat gagatgctca aaatcctgca
    52801 gcaggagagc gtcaaagagg tgtaaacttt cctaagccgc cagccaggcg gcttttttaa
    52861 caacagcatg gattgactgg agataagatg actgaaacga taaaagtaag cgaatcactt
    52921 gaattacatg ctgttgcgga aagtcacgtc acacctcttt atcagttaat ctgtaaaaat
    52981 aaaacctggt tacagcagtc gctaaactgg ccgcagttcg ttcagagtga agaggacacg
    53041 cgaaaaacgg tgcagggtaa tgtgatgttg catcaacgcg gctatgccaa aatgttcatg
    53101 attttcaaag aagatgaact tatcggcgtt atctcgttta atcgtattga accactgaat
    53161 aaaactgctg aaatcggcta ctggctggac gaatctcatc aggggcaggg gatcatttct
    53221 caggcgctgc aggcattgat tcatcattac gctcagtctg gtgaacttag acgcttcgtg
    53281 atcaaatgtc gggtggacaa tccgcaaagc aaccaggtcg ctttgcgcaa tggttttatc
    53341 cttgaaggtt gcctgaaaca ggctgagttc ctgaatgatg cctatgatga tgtgaactta
    53401 tacgcgcgta ttatcgattc ataataaccc tgccagcggc gttcgcgtaa tgcgctcctc
    53461 gccgttaatg acttttggac cacggacatg aaccgtgtcg ccatcaaagg cttcaacgac
    53521 ggcgtggtcg gttaactcca catggttttc aattatcacc gcacctgaaa tacggctctc
    53581 accttgtatt acaacgtgct cgtcgagcag aatcggtcca ccacgtacca cggcatttcc
    53641 gccaatcaag acatgatgtt ttaacacaca attaccttcc acaatggcat attccgccac
    53701 ctgcgaactg taatgaatcg tcggaatggc atcttcttct atgccagctt tcacctgcgc
    53761 gtgaccgtag actttagcgc aatcgcatag ccagacgttg ttctcttcat taccttcaac
    53821 actggcaaaa tcaaaaactt cggcgcgatg ttcaataaaa gcataccgga cgacggcatc
    53881 gccataaatt tgtgcctggt ggacaatacg cgaggcgcta acccttgcgc gatcataaat
    53941 ttggagcagg agttgatggt cgggcgttaa gccttgtgcg gcgacgatca tagaatgttg
    54001 atcaattaag gcgtgaccaa atattctaca ttgcccgtaa actaatgagt catgaatggt
    54061 gacactgtca cttatgtatg caccctggct gatttcacta ttatcgatcc agacattatc
    54121 cgttgcgtaa acctctcccc acagtacgct ggtgccggtg atgcgcgtat taccggaaat
    54181 caccgttccg ccgaacgcaa tagcattctg gtcgtaaatc cagcaatttc cttcctgtgc
    54241 taacaccgtt tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc
    54301 gctaatggcg ataatctgcc gtaataacac atttttttta gtgccatctt cctgataact
    54361 aaaggctcgc tgttcttcac ttagacgata tttgcgcata gcgttttccc acaggtgact
    54421 tactataacc gtagcaaatt ctgcggctct ggctatgctc gagaaaattc cataaaatgc
    54481 atttcaaata tactttataa attaaacaaa atgagtaaga agatgcagag cgataaagtg
    54541 ctcaatttgc cggcaggcta ctttggtatt gtgttgggga cgatagggat gggatttgcc
    54601 tggcgctatg ccagccaggt ttggcaggtc agccactggt taggggatgg gctggtgatt
    54661 ctggcgatga tcatctgggg attattaact agcgcattta ttacccgact catacgcttt
    54721 ccgcatagcg tgctggcgga agttcgccat ccagtgctga gcagttttgt gagtttgttt
    54781 ccggcaacga cgatgctggt ggcgattggt tttgttccgt ggtttcgccc actggcggtg
    54841 tgcctgttca gttttggtgt cgtggttcag ttggcttatg ccgcctggca aactgcggga
    54901 ttatggcgcg gatctcaccc tgaagaagct accacgcctg gactgtatct gccgacagtt
    54961 gccaacaact ttatcagcgc aatggcctgt ggtgcgttgg gctacaccga cgccggtctg
    55021 gtgtttttag gcgcaggcgt tttctcatgg ctaagccttg aaccggtgat cttgcagcgt
    55081 ctgcgcagtt cgggagaatt acccacggca ctgcggacat cactcggcat tcagctcgct
    55141 cctgcgctgg tggcttgtag tgcctggctg agcgtcaacg gcggcgaggg tgacacgctg
    55201 gcgaaaatgc ttttcggtta tggactgctg caactgctgt ttatgctacg tctgatgcca
    55261 tggtatctct cccagccatt taatgcttca ttctggagtt tctcgttcgg cgtatctgca
    55321 ctggcaacca ccggtttgca tctggggagt ggcagcgata atggattttt ccatacgctg
    55381 gcggtgccgc tgtttatctt taccaatttt attattgcaa tactgctcat ccgtactttt
    55441 gcgcttctga tgcagggaaa attgttagtc agaaccgagc gcgccgtttt aatgaaagca
    55501 gaggacaaag aatgatcatt cgtgacgaaa actattttac tgataaatat gaattaaccc
    55561 gcacacactc tgaagtactg gaagcggtga aagtggttaa accgggtaaa acgctggatc
    55621 tgggctgtgg caatggtcgt aacagtcttt acctggcagc caatggttat gatgttgacg
    55681 catgggataa aaatgccatg agtatcgcca acgtcgagcg cattaaatcc attgaaaatc
    55741 tggataattt acacacccga gtcgttgatc tgaataacct cacatttgat agacagtacg
    55801 attttattct ttcgactgtg gtactgatgt tccttgaggc taaaaccatc ccagggctga
    55861 ttgtcaatat gcaacgttgc actaaacctg gtggttacaa cctgattgtg gcggcgatgg
    55921 ataccgctga ttatccatgt accgtcggct tcccgtttgc cttcaaagag ggagaattac
    55981 gtcgatatta cgaaggctgg gagagggtga aatacaatga agacgtcggc gaactgcacc
    56041 gcaccgacgc caacggtaat cgtattaaac tgcgtttcgc cacgatgctg gcacgtaaaa
    56101 aatgacccgc taagaataag gcgcgtgaaa attccccacg ctgagatgat ttactgttct
    56161 tcttttcggt aagcatattt tttatcgaag ggatgtgaaa ttaatcacag tagtcgaagt
    56221 tttttagcag cttaacttac tgaaatttaa ctactgatga ttgacttagc ccctttttcg
    56281 gcattgacta tgtcgtctga aaaggggctg aaaaatttat tttcaccaac actttttttg
    56341 ccacaacacg aagcggcgct ttttgctata acttagaaag taatataatc atctcaggaa
    56401 actattcatg cgtaccacat catttgcgaa agttgcagct ttatgcggct tgttggctct
    56461 gtctggttgt gcctctaaaa tcacccagcc agataaatat tctggttttt taaacaatta
    56521 ctctgattta aaagaaacaa cctcagcaac aggtaaacct gttttacgtt gggtagaccc
    56581 gagttttgat caaagcaaat atgacagcat cgtctggaac ccaatcactt attatccggt
    56641 accaaaacct tctacccagg tagggcagaa agttctggat aaaattttga actataccaa
    56701 caccgaaatg aaagaagcga tagcgcagcg taaaccactg gttaccaccg ctgggccgcg
    56761 tagtctgatt ttccgtgggg ccattaccgg tgtagatacc agcaaagaag ggctgcaatt
    56821 ctatgaagtg gttcctgttg cattagtggt tgcggggaca caaatggcta caggccaccg
    56881 taccatggat actcgcctct attttgaagg tgagctgatt gatgcagcga ctaataaacc
    56941 ggttatcaaa gtcgttcgtc agggcgaagg taaagacctg aataacgaaa gtacgccaat
    57001 ggctttcgaa aatattaaac aagttattga tgacatggcg accgatgcca ccatgtttga
    57061 cgttaacaaa aagtagttcc agacgcgcca tcgttagatg gcgcttttta tccggtgcgc
    57121 cgtaaaaccc catccttcag ggcggggata taaggcgcgg ttttccacct gaccaggtgt
    57181 ttgctgttgt tcaatgtatt ggcggatgac ggatattggc gcacctccgc agctactggc
    57241 aaaatagcca ggactccaca aaacgccttt gtagtaatac ctgactgcaa tatctggtcg
    57301 atctcgtcgc agtaacctac tcgatacgcc tttgaggctg tttaccagac tggatatcgc
    57361 cagtttggga ggatagttaa ttaacaaatg gacgtgatct ggttcgccat ccatttaaac
    57421 cagttcagct tcaaaatcag cacatacatt tgaaaagtaa gtgcgtagtt tttctgtcgc
    57481 gtcatagtca aaaatctgac gtcggtatct ggtgacaaag accaggtgaa catgcatcag
    57541 gaaaacacaa tgcttccccg tcggatatcg gtttctttat tcatagacca aagtatcatt
    57601 tggcctgtga aacgattaca ggcatttaaa ttccagttaa gacccggtgg tcaacaggag
    57661 cgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcgctt
    57721 cagaatgaga atcatgaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg
    57781 ctggttgagt ggaaaaacgc cactgaaacg caatggctta aagattcgcc ctcacagcca
    57841 ttgcaacagt cactgaaagg ccttgagcgg gcttacaaaa acttcttcca gaagcgggct
    57901 gcttttcccc gattcaaaaa gcggggacag aatgatgcat tccgctaccc gcagggcgtt
    57961 aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat gcgctaccgg
    58021 aacagccgtc aggtcacggg agttgtgaaa aatgtcactg tcagccagtc ctgcggtaag
    58081 tggtacatca gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca
    58141 atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt
    58201 gagcctgtaa acagttttca gaaaaaccag aagacgccgg cgagacttca gcgacagtta
    58261 agccgcaagg tcaaattcag caacaactgg cagaagcaga aacgcaaaat acagcgactg
    58321 cattcctgta tcgcaaatat ccgcagggac taccttcaca aagtcacaac gaccgtcagc
    58381 aaaaaccacg caatgatagt cattgaggat ttgaaggtca gcaacatgtc aaagtcagca
    58441 gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa aatcaggttt aaaccgttcg
    58501 atactggatc agggctggta tgaaatgcgc cgccagcttg aatacaagca gctctggagt
    58561 ggcggtcagg tgcttgctgt tccgccagcg tatacaagcc agcgttgcgc gtgctgtggt
    58621 catacagcga aagaaaatcg tctgtcacaa agtaaattca gatgccaggt atgtggatat
    58681 acagcgaacg ccgatgtaaa cggcgctcgt aacattttag cggcggggca cgccgttctt
    58741 gcctgtggag ggatggtgca gtcaggccgc ccgttgaagc aggaacccac cgaaatgatt
    58801 caggcgacag cctgaacgta gcagggatcc acgtccttca gggcgtggag gatgtcaata
    58861 tcggtttctg tcagcgatta aattcaacac cacgtaacaa acgcctccgg caattaatcc
    58921 ccagaacgca gaaccaatcc cgaccagcgt caatccactt gccgttacca gaaatgccac
    58981 caccgccgcg tcgcgctcac gctcattatg cagcgcctga tacaaactgc cgccaatggt
    59041 actcaacagc gccagacctg ccagcatctg gatccaactt acgggcaggg cagccatcat
    59101 cccggtaatg gcactaccaa acagacctgc gagcaaatag aaaatgcctg caccggcagc
    59161 tgccagccaa cgttgatctt tatccggatg cgcttccggg ctttggcaaa tagccgcggt
    59221 gattgccgca ataccgacgg aataaacgcc gaaaggggaa aaaaccagtg ccagcaatcc
    59281 agtaaataca attaatggcg aaacaggagt tgaatatccg gctgctttca ttgctgcgat
    59341 acccggtgcg ttttgcgatg ccatcgtcac cagaaaaagg gggagtgcaa cgctcaggct
    59401 gtgagcaaac gaaaaatcag gggtaatata agtggggaga acgggtttaa agacaacatc
    59461 agttgtgaca acgtcacctt gcgcgataac gatcacgatc ccaataatca tcgcggcaat
    59521 taccgcatag cgcggcgcaa cggccttggt tgccagccat accagcaaca tacttccaca
    59581 caacgtaaat tgaccgtcca gactggcaaa cgcctgtaaa ccaaagcgta ataaaatccc
    59641 ggcaagcatt gccgccgcaa gcgagtgcgg aataatgcgc atcagacgag caaagagtcc
    59701 cgttatgccg cagaggacta ttaacgcgtt ggtgaaaata aaaacgccga tggcttcgtt
    59761 aagtgttagt ccctgcaatc cggtgaccaa caaagccgcg ccaggcgttg accatgcggt
    59821 gagaacaggt acgcgatacc ataatgtcag agtcagcgta ctgacgccca ttgccagccc
    59881 cagcgccgtc atccagccag agatttgtgc agtggtggct ccggcgacaa tcgctgcttg
    59941 ccagattatt gccgctgaac tggcgtagcc aattaatacc gccagaaacc ccgccagtag
    60001 cgtgggtgga ggaacagaaa acagacgcat agtcacctcg tgcgttatag cgtacaggca
    60061 ccgtagcatt tgtccgttat aacgcacaag tgataaactt ccgttttgcc ggaggagtcg
    60121 catggaaaat ctcgctcgct ttttatccac cacacttaaa caactacgcc agcagcgcgg
    60181 ctggagtctt tcgcgactgg cagaagcgac gggcgtttct aaagcaatgt tagggcagat
    60241 tgagcgtaat gagtccagtc cgacggtcgc gacattatgg aaaattgcca ccgggttgaa
    60301 cgtaccattt tcaacattta tttctccgcc gcagcccgct acgccttcag tttatgatcc
    60361 acaacagcac gcgatggtga tcacttcgct gtttccttat gatccacaac tctgctttga
    60421 acacttctcg attcagatgg cacccggtgc aataagcgaa tcgacgcccc atgagaaggg
    60481 ggtaatcgaa catgtcgtcg tcattgatgg acaacttgat ctgtgcgttg atggggaatg
    60541 gcagaccctt aattgtgggg aaggcgttcg atttgctgca gacgtcacgc atatctatcg
    60601 taatggtggg gagcaaaccg tacattttca ttccctcatc cattacccgc gcagttaagc
    60661 gggaaaacta tttcgcaacg tcgcgcttgt ggctaaaata gccgccattt ttcagctact
    60721 ggataagaat gtgaccgtat ctctcatcga cttgaactgt taagcccggc acgcgatgcc
    60781 gccattgccc gcgaagctat tttgcacggt gccgatgctg tttatatcgg cggccctggt
    60841 tttggtgccc gtcataatgc cagtaatagc ctgaaagata ttgccgagct ggtgccgttt
    60901 gcccatcgtt atggtgcaaa aattttcgtc acgcttaaca ccattttgca tgatgatgag
    60961 ctggaacccg cgcaacggct gattactgac ctctaccaga ccggtgtcga tgcgctgatt
    61021 gttcaggata tggggattct ggaacttgat attccgccga ttgaactgca cgccagtacg
    61081 cagtgcgaca ttcgtacagt tgaaaaagcg aagttcctct ctgatgttgg cttcacgcag
    61141 attgtgctgg cgcgagagct gaatcttgat cagatccgcg cgattcacca ggctacggac
    61201 gcgaccattg aattctttat tcatggggca ctgtgcgtgg cctattcggg tcagtgctac
    61261 atttctcatg cgcaaacagg gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt
    61321 ttgccataca cattgaaaga cgatcagggg cgggtggttt cctatgaaaa acatctgctg
    61381 tcgatgaaag ataacgatca gacagccaac ctcggcgcgc tgattgatgc tggcgtacgc
    61441 tccttcaaga ttgaagggcg ttacaaagat atgagctacg tgaagaatat caccgcccat
    61501 tatcgccaga tgctggatgc cattattgaa gaacgtggcg atctgacgcg tgcttcatca
    61561 ggtcgtactg aacatttctt tgttccatcg acggaaaaga ctttccaccg tggtagcaca
    61621 gattattttg tgaatgcccg taaaggcgat attggcgcgt tcgattcgcc gaaatttatc
    61681 ggcctgccgg tgggtgaagt attgaaagtg gcgaaagatc atctcgatgt tgccgtcacc
    61741 gagccactgg caaatggtga tggcctgaac gtgatgatta aacgtgaagt catcggtttt
    61801 cgtgccaata cggtcgagaa aaccagagaa aatcagtacc gcgtctggcc caatgaaatg
    61861 ccagcagatt tgcacaaaat tcgtccacat cacccactaa accgtaatct tgatcataac
    61921 tggcagcagg cactgacaaa aacctccagc gaacgtcggg tggcggtaga cattgaactg
    61981 ggcggctggc aggaacaact gattctgacc ctcaccagtg aagagggtgt cagcatcacg
    62041 catacgctgg acgggcagtt cgacgaagcc aataacgccg aaaaagcaat gaacaatctg
    62101 aaggatggtc tggcaaaact ggggcaaacc ctctattacg cccgcgatgt gcaaattaat
    62161 ttgccggggg cgctgtttgt accaaacagt ctgttaaacc agttccgccg tgaagctgct
    62221 gacatgctgg atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa accggttgct
    62281 gatcctgcgc cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt atacaaccag
    62341 aaagcgcgtg aattttatca tcgctatggt gtgcagctga ttgacgcggc gtatgaagca
    62401 catgaagaga agggcgaagt cccggtgatg atcaccaagc attgtctgcg ctttgccttt
    62461 aatctgtgcc cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa
    62521 ctggttaacg gcgatgaagt attaacgcta aagtttgatt gccgcccatg cgagatgcac
    62581 gtcattggca aaatcaaaaa tcacatactg aaaatgccgt taccgggaag cgtagtggca
    62641 tccgtaagtc cggatgagct gctgaaaaca ttgcctaagc gaaaagggta aaacgccagt
    62701 tttctggtta ctcacaactc attgaatctg catgatattg cctgccgggt aaggcgtctc
    62761 gccgcatccg gcaacaaatg actcaacgtt gtcccgcttc tggtttgcgc gatttttgcc
    62821 agtaaaaatg ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc aattcatcgc
    62881 tgtccgcttc atgacgcagc tgatgatcca cattctttac ccacacaaat tcatgtcctt
    62941 tgtgccctgc catgagttgt cctgagaaca gcgcacacgt taataagaca accgataacg
    63001 ccttcgtaaa catcctgcca cctttttctt accttttgcc gctatgatgc cgatcgtttc
    63061 ttgaggttat tattcagttt tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg
    63121 attttgcatt gcattctgcc gttgcggcga tttagtgcta ttttcgagca aattacacac
    63181 agaggtaaac actaatgttt actttgttga tatactcagc ggcagggagg cgatgtgaaa
    63241 caaagcgagt tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa tggccgcaac
    63301 catttgaaac tcaggtttca tgggaggcgc agtgtcatgc cgcgtcactc ctgcgtaaag
    63361 caatcctgaa acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag
    63421 agacagtcga aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata
    63481 tggtttcttt tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc gctgaagcga
    63541 tggaagcggc aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc
    63601 ttatcccgtt accttcgcca ttaaatagtc atgatcactt tattgaagta cctttgagcg
    63661 tcgcctctaa agtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt
    63721 tagccaggcg aattggcaaa cctaaacagg agattactcg cctatttaac ttgcaccatg
    63781 cgacaaaaat cgacgccgtc cagcttgcgg caaaggcgct tggcaaagag ttatcgctgg
    63841 tgatggttta attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg
    63901 aatcatctgc acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat
    63961 tacgcgagca gattgcgtcg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg
    64021 accaggtggc gctttctggc atgagcttta tgactgtcag ccatgcctat cagttgctcg
    64081 aaagtctggg atatattatc gcgcgaccgc agtcgggtta ttacgttgcg ccacaggcaa
    64141 taaaaatgcc gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca
    64201 cttatatttt tgatatgttg caggccagtc gcgatccgtc ggtcgttccg tttgcctcgg
    64261 cctttcccga cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa
    64321 gcaaaaccgc cacagcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc
    64381 gtcaggctat tgctcgtcgc tatgccttac agggcatcac catttctcct gatgaaattg
    64441 tcattactgc cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg
    64501 gcgattgggt gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc
    64561 tacggctgaa ggcgttatcg gtggcgacgg atgttaaaga agggatcgat cttcaggcgc
    64621 tggaactggc gttgcaggat tatccggtga aagcgtgctg gctgatgact aatagccaga
    64681 acccactcgg atttacctta acgccgcaaa aaaaagcaca actggtggcg ttactcaatc
    64741 agtacaacgt aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa
    64801 aaccgctgcc tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt
    64861 cgaaatgtct ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta
    64921 aaattcaacg cttgcagttg atgagtacgc tttccaccag ctcaccgatg caacttgcgc
    64981 tggtggatta cctttccacg cgccgatacg acgcccatct tcgtcgcctg cgtcgccagc
    65041 ttgcggaacg taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtga
    65101 aaattcatca taatgacagt ggttactttc tctggctgga gctccccgag ccgttagatg
    65161 ccggcgaatt aagcctggcg gcactgacgc atcatatcag tattgcgccg ggtaaaatgt
    65221 tttctaccgg tgaaaactgg tcacgttttt tccgttttaa taccgcgtgg cagtggggag
    65281 agcgtgaaga acaggcggta aaacaattag gcaaacttat tcaagaacgg ctgtaatagc
    65341 gtttaattta attcctctta gattgggtaa tatgaatttc gaatagcagt catatttcct
    65401 aactccttga ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac
    65461 aacctgatta aattttctca ggggcgaagg tgtgcctgca agccgccgtc tatggttaaa
    65521 caaggagata tttttacggc acggcggctg aacaattaat tacgacagga gtaagacctt
    65581 atgagcaaga catttgcccg cagcagcctg tgtgcgctca gcatgacaat aatgaccgct
    65641 cacgccgccg aaccgcctac caatttagat aaaccggaag ggcgactgga tattatcgcc
    65701 tggccgggat acatcgaacg cggacaaact gataaacaat acgactgggt aacgcagttc
    65761 gaaaaagaga caggctgcgc ggtgaatgtg aaaaccgccg cgacttccga tgaaatggtc
    65821 agtctgatga ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgt
    65881 ttgattatgg gtaaacgcgt gcagccgatt aataccgcat tgattcccaa ctggaaaacg
    65941 ctcgatccgc gcgtggttaa aggcgactgg tttaatgttg gcggcaaagt ttacggcaca
    66001 ccttaccaat gggggccgaa cctgctgatg tacaacacta aaaccttccc gacgccgccg
    66061 gatagctggc aagtggtttt tgttgagcaa aatctgccgg acggcaagag caataaaggc
    66121 cgcgttcagg cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc
    66181 actcagccgc agttgggcat cagcgatccg tatcaactca ccgaagaaca gtaccaggcg
    66241 gtgctgaaag tgctgcgcgc tcaacacagt ttgatccatc gctactggca tgacactacc
    66301 gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt ccagtgcctg gccctatcag
    66361 gccaacgccc tgaaagccga aggccagcct gttgctaccg ttttcccgaa ggagggtgtt
    66421 accggttggg ctgataccac catgctgcat agcgaagcga aacatccggt ttgcgcctac
    66481 aaatggatga actggtcatt aacgccaaaa gtgcagggcg atgtggcggc ctggtttggc
    66541 tcgttaccgg tagtgccgga agggtgtaaa gccagtccgt tattaggcga aaaaggttgt
    66601 gaaaccaacg gttttaacta tttcgacaaa atcgccttct ggaaaacgcc tatagcagaa
    66661 gggggcaagt ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt
    66721 cgctaacttc gctggggtgc tttatgacgt acgcagtgga gtttgacaac gtctcgcggt
    66781 tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct
    66841 tctctatgct ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct
    66901 tcgaacagct ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac
    66961 cgtgggagcg ggacgtgaat actgtctttc aggactacgc gctatttccg catatgtcga
    67021 ttcttgacaa tgtcgcctat gggctgatgg tcaaaggcgt gaataaaaag cagcggcacg
    67081 caatggcgca agaggcgctg gagaaagtgg cgttggggtt tgtacatcaa cgtaaaccgt
    67141 cacaactttc tggtggtcag cgccagcggg ttgctatcgc cagagcattg gtgaatgaac
    67201 cgcgcgtatt gctgttggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga
    67261 tgcagctgga actgaaaaaa ctgcaacagt ctctcggtat cacttttatc ttcgttaccc
    67321 acgatcaggg cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca
    67381 ttgagcaggt cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg
    67441 gattcgtcgg gacatccaac gtttttgatg gactgatggc agagaaactt tgtggcatga
    67501 cgggaagctt cgccctgcga ccggaacata tccgcctcaa cacccctggt gaactgcagg
    67561 ccaatggcac gatccaggcg gtgcaatatc agggcgcggc aactcgtttt gaactgaaat
    67621 tgaacggcgg tgaaaaactg cttgtgagtc aggccaatat gacaggcgaa gaactgcctg
    67681 ccacgctcac gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc
    67741 tggttgagga gaggtgaatg gcgatgaatg tattgcaatc accttcacgt ccaggtctgg
    67801 gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg gctgttttta ctgctgcttg
    67861 gcccgctaat gtggtttggc attgtctatt tcggctcgct gctgacactg ttatggcagg
    67921 gattttatac ttttgacgat ttcaccatgt cggtaacgcc ggaactgacg ctggcgaata
    67981 tccgtgcgct gtttaatccg gcgaattacg acatcattct ccgcacgctg actatggctg
    68041 tggcagtcac tatcgccagc gccattctgg cttttccaat ggcgtggtat atggcgcgct
    68101 ataccagcgg gaaaatgaaa gctttttttt atattgcggt aatgttgccg atgtgggcga
    68161 gctacattgt taaagcctat gcctggacgt tattgctggc aaaagatggc gtggctcagt
    68221 ggtttttaca acatcttggg ctggaaccac tgctgacggc gttccttaca ttacctgcgg
    68281 tgggcggaaa tacgctgtca acttccgggc tggggcgctt tctggtgttt ctctatatct
    68341 ggttgccgtt catgatcctg cccgttcagg cggcgcttga gcgtttgccg ccgtcattgt
    68401 tgcaggcgtc ggctgatctc ggcgcacgtc cacgacaaac ctttcgctat gtggtgctgc
    68461 cgctggcaat cccgggtatt gccgctggct ctatctttac cttctcactc acactgggcg
    68521 attttatcgt cccgcagctg gttggtcctc caggatattt tatcggcaat atggtttatt
    68581 cccagcaggg ggcgattggc aatatgccga tggcggcggc attcaccctg gtgccgattg
    68641 ttctcatcgc actgtacctg gcgttcgtga aacgtctggg agcgttcgat gcactctgaa
    68701 cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg tggttttcct acattttccc
    68761 atcctgatta tcgccgctta tgcgtttaac actgaagatg cggcgtttag ttttccaccg
    68821 cagggcctga cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc
    68881 gtgacactgt cacttaaagt ggcggcgctg gcgacattaa tggcgctggt gttagggacg
    68941 ctggcaactg ccgcgctgtg gcgacgagac tttttcggca aaaacgccat ttcgctgtta
    69001 ctgctgctgc ccattgcgct gccgggcatt gtcactggtc tggcgctatt aaccgctttt
    69061 aaaaccatca atctggagcc gggatttttc accatcgtgg tcggtcatgc gactttttgc
    69121 gtagtggtgg tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcgtg gagtctggtt
    69181 gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct tccgctacgt agtgttgccg
    69241 aatctcagtt cggcgctact ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa
    69301 atcatcgtta cgacttttac ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat
    69361 cagcttgggc gaccgcgtga tgtaccggta actaacgtgg tggcactgct ggttatgttg
    69421 gtaacaacct tgccgatcct gggggcctgg tggctaaccc gcgaaggcga caatggtcaa
    69481 taaccactga tacaggaata tgctatgcaa cataagttac tgattaacgg agaactggtt
    69541 agcggcgaag gggaaaaaca gcctgtctat aatccggcaa cgggggacgt tttactggag
    69601 attgccgagg catccgcaga gcaggtcgat gctgctgtgc gcgcggcaga tgcagcattt
    69661 gccgaatggg ggcaaaccac gccgaaagtg cgtgcggaat gtctgctgaa actggctgat
    69721 gttatcgaag aaaatggtca ggtttttgcc gaactggagt cccgtaattg tggcaaaccg
    69781 ctgcatagtg cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg ctttttcgcg
    69841 ggtgcggcgc gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg tcatacttcg
    69901 atgatccgtc gcgatccgtt gggggtcgtg gcttctatcg caccgtggaa ttatccgctg
    69961 atgatggcag cgtggaaact ggctccggcg ctggcggcag ggaactgcgt agtgcttaaa
    70021 ccatcagaaa ttaccccgct gaccgcgttg aagttggcag agctggcgaa agatatcttc
    70081 ccggcaggcg tgattaacgt actgtttggc agaggcaaaa cggtgggtga tccgctgacc
    70141 ggtcatccca aagtgcggat ggtgtcgctg acgggctcta tcgccaccgg cgagcacatc
    70201 atcagccata ccgcgtcgtc cattaagcgt actcatatgg aacttggtgg caaagcgcca
    70261 gtgattgttt ttgatgatgc ggatattgaa gcagtggtcg aaggtgtacg tacatttggc
    70321 tattacaatg ctggacagga ttgtactgcg gcttgtcgga tctacgcgca aaaaggcatt
    70381 tacgatacgc tggtggaaaa actgggtgct gcggtggcaa cgttaaaatc tggtgcgccg
    70441 gatgacgagt ctacggagct tggaccttta agctcgctgg cgcatctcga acgcgtcagc
    70501 aaggcagtag aagaggcgaa agcgacaggg cacatcaaag tgatcactgg cggtgaaaag
    70561 cgcaagggta atggctatta ctatgcgccg acgctgctgg ctggcgcatt acaggacgat
    70621 gccatcgtgc aaaaagaggt atttggtcca gtagtgagtg ttacgccctt cgacaacgaa
    70681 gaacaggtgg tgaactgggc gaatgacagc cagtacggac ttgcatcttc ggtatggacg
    70741 aaagatgtgg gcagggcgca tcgcgtcagc gcacggctgc aatatggttg tacctgggtc
    70801 aatacccatt tcatgctggt aagtgaaatg ccgcacggtg ggcagaaact ttctggttac
    70861 ggcaaggata tgtcacttta tgggctggag gattacaccg tcgtccgcca cgtcatggtt
    70921 aaacattaag ttagatgtta gtggctgtta taggacggcc actatcgttt taggttctga
    70981 gtttaatggc ggttaagtat tagaatgatt acattaataa ctagcgtaaa aattaggcta
    71041 aatgtttgat ggcgattttt catcctgt
//
LOCUS       DAZGCS010000010        70312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGCS010000010 DAZGCS010000000
VERSION     DAZGCS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.010"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(21..173)
                     /locus_tag="ACHK01_000695"
     CDS             complement(21..173)
                     /locus_tag="ACHK01_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095692.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            191..382
                     /locus_tag="ACHK01_000696"
     CDS             191..382
                     /locus_tag="ACHK01_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO8095693.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            693..1211
                     /locus_tag="ACHK01_000697"
     CDS             693..1211
                     /locus_tag="ACHK01_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8095694.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            1227..1766
                     /locus_tag="ACHK01_000698"
     CDS             1227..1766
                     /locus_tag="ACHK01_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO8095695.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1861..3438)
                     /gene="guaA"
                     /locus_tag="ACHK01_000699"
     CDS             complement(1861..3438)
                     /gene="guaA"
                     /locus_tag="ACHK01_000699"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO8095696.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3507..4973)
                     /gene="guaB"
                     /locus_tag="ACHK01_000700"
     CDS             complement(3507..4973)
                     /gene="guaB"
                     /locus_tag="ACHK01_000700"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO8095697.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            5135..6505
                     /gene="xseA"
                     /locus_tag="ACHK01_000701"
     CDS             5135..6505
                     /gene="xseA"
                     /locus_tag="ACHK01_000701"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO8095698.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6502..6717)
                     /locus_tag="ACHK01_000702"
     CDS             complement(6502..6717)
                     /locus_tag="ACHK01_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO8095699.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6786..8258)
                     /gene="der"
                     /locus_tag="ACHK01_000703"
     CDS             complement(6786..8258)
                     /gene="der"
                     /locus_tag="ACHK01_000703"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO8095700.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8376..9554)
                     /gene="bamB"
                     /locus_tag="ACHK01_000704"
     CDS             complement(8376..9554)
                     /gene="bamB"
                     /locus_tag="ACHK01_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO8095701.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9565..10185)
                     /gene="yfgM"
                     /locus_tag="ACHK01_000705"
     CDS             complement(9565..10185)
                     /gene="yfgM"
                     /locus_tag="ACHK01_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO8095702.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(10203..11477)
                     /gene="hisS"
                     /locus_tag="ACHK01_000706"
     CDS             complement(10203..11477)
                     /gene="hisS"
                     /locus_tag="ACHK01_000706"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO8095703.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11588..12706)
                     /gene="ispG"
                     /locus_tag="ACHK01_000707"
                     /gene_synonym="gcpE"
     CDS             complement(11588..12706)
                     /gene="ispG"
                     /locus_tag="ACHK01_000707"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO8095704.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12733..13746)
                     /gene="rodZ"
                     /locus_tag="ACHK01_000708"
     CDS             complement(12733..13746)
                     /gene="rodZ"
                     /locus_tag="ACHK01_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO8095705.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(14031..15185)
                     /locus_tag="ACHK01_000709"
     CDS             complement(14031..15185)
                     /locus_tag="ACHK01_000709"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO8095706.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15335..15766)
                     /gene="ndk"
                     /locus_tag="ACHK01_000710"
     CDS             complement(15335..15766)
                     /gene="ndk"
                     /locus_tag="ACHK01_000710"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO8095707.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15915..18227)
                     /gene="pbpC"
                     /locus_tag="ACHK01_000711"
     CDS             complement(15915..18227)
                     /gene="pbpC"
                     /locus_tag="ACHK01_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO8095708.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPDQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(18228..23189)
                     /gene="yfhM"
                     /locus_tag="ACHK01_000712"
     CDS             complement(18228..23189)
                     /gene="yfhM"
                     /locus_tag="ACHK01_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO8095709.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            23396..24241
                     /gene="sseA"
                     /locus_tag="ACHK01_000713"
     CDS             23396..24241
                     /gene="sseA"
                     /locus_tag="ACHK01_000713"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO8095710.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24903..25028
                     /gene="timP"
                     /locus_tag="ACHK01_000714"
     CDS             24903..25028
                     /gene="timP"
                     /locus_tag="ACHK01_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO8095711.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(25060..25836)
                     /gene="sseB"
                     /locus_tag="ACHK01_000715"
     CDS             complement(25060..25836)
                     /gene="sseB"
                     /locus_tag="ACHK01_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO8095712.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25978..27261)
                     /gene="pepB"
                     /locus_tag="ACHK01_000716"
     CDS             complement(25978..27261)
                     /gene="pepB"
                     /locus_tag="ACHK01_000716"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO8095713.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(27439..27639)
                     /gene="iscX"
                     /locus_tag="ACHK01_000717"
     CDS             complement(27439..27639)
                     /gene="iscX"
                     /locus_tag="ACHK01_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO8095714.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27651..27986)
                     /gene="fdx"
                     /locus_tag="ACHK01_000718"
     CDS             complement(27651..27986)
                     /gene="fdx"
                     /locus_tag="ACHK01_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO8095715.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27988..29838)
                     /gene="hscA"
                     /locus_tag="ACHK01_000719"
     CDS             complement(27988..29838)
                     /gene="hscA"
                     /locus_tag="ACHK01_000719"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO8095716.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29855..30370)
                     /gene="hscB"
                     /locus_tag="ACHK01_000720"
     CDS             complement(29855..30370)
                     /gene="hscB"
                     /locus_tag="ACHK01_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO8095717.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(30466..30789)
                     /gene="iscA"
                     /locus_tag="ACHK01_000721"
     CDS             complement(30466..30789)
                     /gene="iscA"
                     /locus_tag="ACHK01_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO8095718.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30806..31192)
                     /gene="iscU"
                     /locus_tag="ACHK01_000722"
     CDS             complement(30806..31192)
                     /gene="iscU"
                     /locus_tag="ACHK01_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO8095719.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(31220..32434)
                     /gene="iscS"
                     /locus_tag="ACHK01_000723"
     CDS             complement(31220..32434)
                     /gene="iscS"
                     /locus_tag="ACHK01_000723"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO8095720.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(32546..33034)
                     /gene="iscR"
                     /locus_tag="ACHK01_000724"
     CDS             complement(32546..33034)
                     /gene="iscR"
                     /locus_tag="ACHK01_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO8095721.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(33304..34044)
                     /gene="trmJ"
                     /locus_tag="ACHK01_000725"
     CDS             complement(33304..34044)
                     /gene="trmJ"
                     /locus_tag="ACHK01_000725"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO8095722.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            34163..34966
                     /gene="suhB"
                     /locus_tag="ACHK01_000726"
     CDS             34163..34966
                     /gene="suhB"
                     /locus_tag="ACHK01_000726"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO8095723.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            35111..35965
                     /locus_tag="ACHK01_000727"
     CDS             35111..35965
                     /locus_tag="ACHK01_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8095724.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            36156..37436
                     /gene="csiE"
                     /locus_tag="ACHK01_000728"
     CDS             36156..37436
                     /gene="csiE"
                     /locus_tag="ACHK01_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO8095725.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(37428..38567)
                     /locus_tag="ACHK01_000729"
     CDS             complement(37428..38567)
                     /locus_tag="ACHK01_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO8095726.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGESRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38727..39617)
                     /gene="hcaR"
                     /locus_tag="ACHK01_000730"
     CDS             complement(38727..39617)
                     /gene="hcaR"
                     /locus_tag="ACHK01_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO8095727.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            39753..41114
                     /gene="hcaE"
                     /locus_tag="ACHK01_000731"
     CDS             39753..41114
                     /gene="hcaE"
                     /locus_tag="ACHK01_000731"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO8095728.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            41111..41629
                     /gene="hcaF"
                     /locus_tag="ACHK01_000732"
     CDS             41111..41629
                     /gene="hcaF"
                     /locus_tag="ACHK01_000732"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO8095729.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41629..41949
                     /locus_tag="ACHK01_000733"
     CDS             41629..41949
                     /locus_tag="ACHK01_000733"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO8095730.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41946..42758
                     /gene="hcaB"
                     /locus_tag="ACHK01_000734"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41946..42758
                     /gene="hcaB"
                     /locus_tag="ACHK01_000734"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO8095731.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42768..43970
                     /gene="hcaD"
                     /locus_tag="ACHK01_000735"
     CDS             42768..43970
                     /gene="hcaD"
                     /locus_tag="ACHK01_000735"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO8095732.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            44067..44489
                     /locus_tag="ACHK01_000736"
                     /pseudo
     CDS             44067..44489
                     /locus_tag="ACHK01_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            complement(44537..45409)
                     /locus_tag="ACHK01_000737"
     CDS             complement(44537..45409)
                     /locus_tag="ACHK01_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8095733.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(45421..46515)
                     /locus_tag="ACHK01_000738"
     CDS             complement(45421..46515)
                     /locus_tag="ACHK01_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8095734.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(46548..47546)
                     /locus_tag="ACHK01_000739"
     CDS             complement(46548..47546)
                     /locus_tag="ACHK01_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8095735.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(47571..49082)
                     /locus_tag="ACHK01_000740"
     CDS             complement(47571..49082)
                     /locus_tag="ACHK01_000740"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8095736.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(49105..50088)
                     /locus_tag="ACHK01_000741"
     CDS             complement(49105..50088)
                     /locus_tag="ACHK01_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8095737.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIY
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(50185..53466)
                     /locus_tag="ACHK01_000742"
     CDS             complement(50185..53466)
                     /locus_tag="ACHK01_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO8095738.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            53584..54777
                     /locus_tag="ACHK01_000743"
     CDS             53584..54777
                     /locus_tag="ACHK01_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO8095739.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54841..56094)
                     /gene="glyA"
                     /locus_tag="ACHK01_000744"
     CDS             complement(54841..56094)
                     /gene="glyA"
                     /locus_tag="ACHK01_000744"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO8095740.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            56422..57612
                     /gene="hmpA"
                     /locus_tag="ACHK01_000745"
     CDS             56422..57612
                     /gene="hmpA"
                     /locus_tag="ACHK01_000745"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO8095741.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57657..57995)
                     /gene="glnB"
                     /locus_tag="ACHK01_000746"
     CDS             complement(57657..57995)
                     /gene="glnB"
                     /locus_tag="ACHK01_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO8095742.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(58056..59390)
                     /gene="glrR"
                     /locus_tag="ACHK01_000747"
     CDS             complement(58056..59390)
                     /gene="glrR"
                     /locus_tag="ACHK01_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO8095743.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(59380..60093)
                     /gene="qseG"
                     /locus_tag="ACHK01_000748"
     CDS             complement(59380..60093)
                     /gene="qseG"
                     /locus_tag="ACHK01_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO8095744.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(60258..61685)
                     /gene="qseE"
                     /locus_tag="ACHK01_000749"
     CDS             complement(60258..61685)
                     /gene="qseE"
                     /locus_tag="ACHK01_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO8095745.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(62260..66147)
                     /gene="purL"
                     /locus_tag="ACHK01_000750"
                     /gene_synonym="purI"
     CDS             complement(62260..66147)
                     /gene="purL"
                     /locus_tag="ACHK01_000750"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO8095746.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            66405..67961
                     /gene="mltF"
                     /locus_tag="ACHK01_000751"
     CDS             66405..67961
                     /gene="mltF"
                     /locus_tag="ACHK01_000751"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO8095747.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67958..68461)
                     /gene="tadA"
                     /locus_tag="ACHK01_000752"
     CDS             complement(67958..68461)
                     /gene="tadA"
                     /locus_tag="ACHK01_000752"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO8095748.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(68519..69154)
                     /gene="yfhb"
                     /locus_tag="ACHK01_000753"
     CDS             complement(68519..69154)
                     /gene="yfhb"
                     /locus_tag="ACHK01_000753"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO8095749.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            69363..70211
                     /locus_tag="ACHK01_000754"
     CDS             69363..70211
                     /locus_tag="ACHK01_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8095750.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRQSEA
                     LMKKLV"
BASE COUNT        16729 a        19340 c        17646 g        16597 t
ORIGIN      
        1 gaattattac gtttatcatg ttaattcatc attattacat catcattgta aataattaaa
       61 ttaacttcca taacattaaa atatgtatcc actgacgctt ttttacataa cgaagaattg
      121 accattttgt cctgttgtgc cttaatgtaa gtaccgtcca cagcgtggga catacttcaa
      181 ggaacctttt gtgagtcagg caaccagtat gcgaaaacga caccgattta acagtcgcat
      241 gacccgtatc gtactgctca tcagctttat cttcttcttt ggccgtttta tctactcgtc
      301 cgtcggtgcc tggcagcacc atcagagcaa aaaagaagct cagcaatcca cactctccgt
      361 cgaatcaccg gtacaacgtt agcggttacc ttctccactt tcacagaacc taacggcact
      421 tcgctgtcgg atgcttttgc tctttgggat tatcaaagcg gcagatattc tttcatctta
      481 aattttacgt ctttgtcctg actgatgttt atcctgtttg gctgcgaaat aaatataaaa
      541 ttaatatata tgttgtaatg atatattttt ataaattatt cccggcgtga attttaataa
      601 atttaatcta tccctttata cgcaatacat ttactttcct cttttgatga tcttaaatgt
      661 cttatttttc gtaatgtgta taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc
      721 ctgtagcaat gttagcgtca ttcactctgg caggatgcca gtcaaatgct gacgatcatg
      781 ccgccgatgt ttatcaaacc gatcaactga ataccaaaca agaaactaaa accgttaata
      841 ttatttccat tcttcccgca aaagttgccg tagacaactc ccaaaataaa cggaacgcac
      901 aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt tatcggccac aacgtcggtt
      961 ctggcagcaa ttccggaacg acggcagggg cagttggcgg cggagctgta ggcgcggcag
     1021 cgggttctat ggtgaatgat aaaaccttag tggaaggtgt ttctttaacg tataaggaag
     1081 gcaccaaagt gtatacctcc acccaggtgg gtaaagagtg ccagtttacg acaggtttag
     1141 ccgttgttat taccacgacg tataacgaaa cgcgtattca gccaaatact aaatgtcctg
     1201 aaaagagcta ataatcagga ggagtcatga agaaagtttt tctttgcgcc atcttggcct
     1261 ccttaagcta tccggctatc gcctcatcat tgcaggatca actctcggct gtcgcagaag
     1321 cggaacagca aggtaaaaat gaagagcaaa agcagcatga cgaatgggtc gcggagcgca
     1381 acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc ccaggccgcc gctaacaaaa
     1441 gagcggcaac ggcagcagca aataagaaag ctcgtcagga taaactggac gccgaagcct
     1501 ctgcggacaa aaaacgcgat caaagttatg aagatgagct acgcagctta gagattcaga
     1561 aacaaaaact ggcgctggca aaagaagaag cccgcgttaa gcgagaaaac gaatttatcg
     1621 atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcca
     1681 acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag cgtgggcaaa gcagaagaga
     1741 acaaatcgga cagctggttt aattaatcga tgttagtaac ttcaagccta tgattcttga
     1801 agataaaaaa ccctctgtag taacagaggg ttttgttcat tcatagtgca gggattaaaa
     1861 tcattcccac tcaatggtag ctggcggctt gccgctgatg tcatacacca cgcgggaaat
     1921 accgttcact tcattgataa tgcggttgga aacgcgaccg aggaaatcgt acggcagatg
     1981 cgcccagtgt gcggtcataa agtcgatggt ttcgacagca cgcagagaga caacccagtc
     2041 atacttacga ccatcgccca ttacgccaac ggaacgtacc ggcaggaaca cagtgaacgc
     2101 ctggctgact ttgtcgtaca ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc
     2161 acggcgcagc aggtcacagt actctttctt cacttcaccc agcacacgaa cgccaaggcc
     2221 tggtcccggg aacgggtgac ggtacagcat gtcgtacggc aggcccagct ccagaccaat
     2281 cttacgcact tcgtctttga acagctcttt cagcggttca accaggccca tcttcatctc
     2341 tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg tgtgctttac cggttgcaga
     2401 agccgcagat tcgataacgt cagggtagat ggtgccctgc gccagccact tcacgtcttc
     2461 cagtttcagc gcttcttcat cgaatacttc aacgaaaacg cgcccgatga ttttacgttt
     2521 tgcttccgga tcgttttcgc cagccagcgc tgacaggaag cgatcttctg ccggtacgtg
     2581 aacaatgtta agaccaaagt gatcgccaaa catatccaga acctgctctg cttcgttgag
     2641 acgcagcagg ccgttgtcga cgaatacgca agtcaggttt ttaccgatag cgcggtgcag
     2701 cagcattgcg gttacggagg aatccacacc accagagagg ccgaggatga ctttatcgtc
     2761 gcctacctgc tcgcggatgc gagctacagc atcgtcgata attttcgctg gcgtccacag
     2821 ggcttcacac tggcagatat cacgcacaaa acgctccagc atgcgcatac cctggcgggt
     2881 gtgagtcact tccgggtgga actgtacgcc atagaagcgt ttttcttcgt tagccataat
     2941 ggcaaacggg cagctttcgg tgctggctac ggtgacgaag tcggacggga tagcggtaac
     3001 tttatcgccg tggctcatcc agacatcgag cagcggttta ccgtctgcgg tcagcgcatc
     3061 ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca acctgcgcgt agccaaattc
     3121 acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt gccatggtct gcatgccata
     3181 gcaaacgccg aataccggta cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt
     3241 ttcttcggtg gtgctttccg ggccgccgga aagaataatg ccgcttggat tgaagtcacg
     3301 aatttgtgct tctgtcacat cccacgccca cagttcgcag taaacaccca gctcacgcac
     3361 gcggcgcgca accagttgag tgtactgaga accgaagtcc agaatgagga tgcgatgctt
     3421 atgaatgttt tccgtcattg acgcttattc cgaggcaagt gaaacagata atataaatcg
     3481 cccgacatga agtcgggcga agagaatcag gagcccagac ggtagttcgg ggactcttta
     3541 gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg caccgctgat acgtacaaac
     3601 tccgctttag tacgcagttc gtcgatagta ccacagccgg tcagacccat acaggagcgc
     3661 aggccgccca tctgctggtg aatgatctct ttcaggcgac ctttataagc tacgcgacct
     3721 tcgatacctt ccggcaccag tttgtcggca gcgttatcgc tctggaaata acggtcagag
     3781 gaacctttgg acatcgcgcc cagggaaccc ataccacggt aagatttgta agaacggccc
     3841 tggtagagtt cgatttcacc cggagattct tcagtacccg ccagcatgga acctaccatc
     3901 accgcgcttg cgccagctgc gatagctttg gcgatgtcgc cggagaagcg aataccacca
     3961 tctgcgataa ccggaatacc ggtgccttcc agggcttcaa ctgcgtcagc aacggcagtg
     4021 atctgcggaa cacctacgcc agtaacgata cgagtcgtac agatagaacc agggccgata
     4081 ccgactttaa ccgcgctgca accagcttct gccaaagcgc gtgcacctgc agctgttgcc
     4141 acgttgccgc cgatgatttg cagatccgga tatttagcac gggtttcacg gatacgctgc
     4201 aaaactcctt ctgagtgacc gtgggaggag tcgatcagca gaacgtcaac gccagcggca
     4261 accagcgcgt caacacgctc ttcgttaccc gcacctgcgc caactgcagc accaacacgc
     4321 agacggcctt gctcgtcttt acaggcgttc ggtttacgtt ccgctttctg gaagtctttc
     4381 acggtgatca tgccgatcag gtggaattcg tcatcaacca ccagcgcttt ttcaacgcgt
     4441 ttttcgtgca tttttgccag caccacttca cgggcttcac cttcacgcac ggtgaccaga
     4501 cgctctttcg gcgtcatgta aacgctaacc ggctggttca ggtcggtaac aaaacgcacg
     4561 tcacgaccgg tgataatacc caccagttcg ttttcttcgg taacgaccgg atagcccgca
     4621 aaaccgttac gctcggtcag ttctttcact tcgcgcagcg tcgtggttgg cagaacagtc
     4681 tgcggatcag tcaccacacc agattcgtgt tttttcacac ggcgaacttc ttctgcctgg
     4741 cgttcaatgg acatgttttt gtggataaag ccgataccgc cttcctgagc cagagcaata
     4801 gccaggcgcg cttccgttac ggtatccatt gctgcggaaa gcatagggat attcagacga
     4861 atagttttcg tcagctgggt gctgaggtca gcagtattcg gcagaacggt agaatgagca
     4921 ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag cgatacgtag catgggcaat
     4981 atctcgacca gagtggttaa taaatattgc cgcggcatta tacagagcgt aaccgattgc
     5041 atctacccct ttttgcaaaa aatgcttgct atccccgaag ggcgggttac tatcgactga
     5101 ataacctgct gattttgaat ttgatctcgc tcacatgtta ccttctcaat cccctgcaat
     5161 ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg cttgagcatg agatgggaca
     5221 ggtttggatc agcggcgaaa tctctaattt cacacaaccg gcttccggtc actggtactt
     5281 tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg ttccgcaaca gcaaccgtcg
     5341 ggtgaccttc cgtccacagc atgggcaaca agttttagtt cgcgccaata ttacgctcta
     5401 cgagccgcgc ggcgactacc agataatcgt tgagagtatg cagccggccg gtgaagggct
     5461 gctgcaacag aagtacgaac agctcaaagc gaagttgcag gctgaaggat tgttcgatct
     5521 gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac
     5581 cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt cgcgatcctt ctctaccggt
     5641 gatcatctac cccaccgccg ttcagggcga tgacgcgccg gggcaaactg ttcgcgccat
     5701 tgaactggcg aatcagcgca atgagtgcga cgtgttgatc gttgggcgcg gcggcggttc
     5761 gctggaagat ttatggagtt ttaacgacga acgcgtagcg cgggcgattt ttgccagccg
     5821 cattccggtc gtcagcgccg tcgggcatga gacggatgtg accattgccg attttgttgc
     5881 cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta gtgagccgta atcagcaaga
     5941 gttactgcgc caggtgcaat cgacccgtca acggctggag atggcgatgg attattatct
     6001 cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga ttacagcaac agcatccgca
     6061 gctccggctg gcacgccagc aaaccatgct tgagcgcctg caaaagcgga tgagctttgc
     6121 gctggaaaat caacttaagc gtaccgggca acagcagcag cggttaacac agcggctgaa
     6181 tcagcaaaat ccacagccga agattcatcg cgcgcaaacg cgcattcagc aactggaata
     6241 tcgtttagca gaaaccctgc gcgtacagct tagcgccacg cgtgaacgtt tcggtaatgc
     6301 agtaacgcac ctcgaagccg taagcccact gtcaacgctg gcgcgtggat acagcgttac
     6361 tactgctact gacggcaatg tactgaaaaa agtgaagcaa gttaaagcgg gtgaaatgct
     6421 aaccacacgt ctggaagacg gctggataga aagtgaagta aaaaacatcc agccggtaaa
     6481 aaaatcgcgt aaaaaggtgc attaagccag cacgaattca acgcgttttt tcgatatcaa
     6541 gccatgcccg tgctgacaga agtaatctac tgcaccgcag gcttttaaca cctgcaacgg
     6601 ttgatggcag tctggacaaa gagctttcat ttcaataatt tccccgcagg atgggcaacg
     6661 cgcatggccg ttatcctgat caagcacatg ctgacattgt ggacaatgca gttccatatg
     6721 aattcctccg gtagccatcc ctctacattc atagagggaa tggcagataa aatacttacg
     6781 gataattatt tatttttctt gatgtgcttc atcagacgct tacgtttacg catctgggtt
     6841 ggcgtcaggg tgttacgctt gttcgcatac gggttttccc cttctttgaa ctgaatacga
     6901 atcggcgatc ccattacgtc cagcgatttg cggaagtagt tcatcaagta gcgcttgtag
     6961 gaatcaggca ggtctttcac ctgattaccg tgaatcacca caatcggcgg gttataacca
     7021 ccagcgtggg catatttcag cttcacacga cgaccgcgta ccagcggcgg ttggtgatct
     7081 tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc ccacacgacg ggtggagctg
     7141 tcatacgctt cacgtactga ttcaaacaag ttaccaacac cactgccgtg caaggcagag
     7201 ataaagtgca cacgagcaaa atcgataaag cccagacgga agtccagcgt ttctttcacc
     7261 tgctctttca cttcctggct caggccatcc cacttattca ccacaatgac aagtgaacgc
     7321 ccactattga gaataaagcc cagcagcgag agatcctgat cggaaatacc ttcgcgcgca
     7381 tcaatcacta acatcaccac gttggcatct tcaatggcct gcaacgtttt gattacggag
     7441 aatttctcta cagcatcggt gattttgccg cgtttacgta cgccagcggt gtcaatgagc
     7501 acatactcac gtccatcgcg ttccattgga atgtagatac tgtcacgcgt cgtgccaggc
     7561 atgtcataaa caacaacgcg ctcttcacca agaatacggt tagtgagtgt agacttacct
     7621 acgttcggac gacccacaat cgccagtttg atcggcagac tttgcgggtc gaagtcgtct
     7681 tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg cccagtattc agcgtcttcg
     7741 tcgacttcct cttgcggtgc gagatcttcc atccacggca gcagcacatg ctccagcaga
     7801 cttaatacgc cacgaccgtg agacgcggcg atcgggtaga tttcacctaa accaagcgag
     7861 tagaaatcaa ccactgcctg atcgggatcc agaccgtcag ttttgtttgc caccaggaag
     7921 gtcggttttt cacgggagcg cagatgtttg gcaatcgctt catctgccgg catcaggccc
     7981 gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt caatcgccag cagcgactgt
     8041 tccgccatgc gggtttctac accgtcttct gtgccatcaa tcccgccggt atcaatacag
     8101 ataaactcac ggccttcaat ttccgcacga ccgtacttac ggtcacgagt cagacccggg
     8161 aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt taaataacgt ggattttcct
     8221 acgttagggc gcccgacaag cgcgaccaca ggtaccatgt ttaaagcctc atttttataa
     8281 atcatcagac aacgcacgct atattcgcgt cgttgttaaa aacaggaaaa cggcccctgt
     8341 ccaggagccg ttttcaaagt gaacgacaga gacgattaac gtgtaataga gtacacggtt
     8401 ccgtcttttg cctggatcag cagtttgccg tcagcggcaa ccggttcagt ctggaaaccg
     8461 gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt cgacgttaat ccagtgcaga
     8521 taaccttcac tgtcaccgac caccaggttg ccattataca gcaccggaga agtcagcagg
     8581 cgatgcagca gatcgctttg tgcccacagc gtaacgccgc catcaatggt caacgccatc
     8641 acccggtcat tttgatcgac cagatagatg cgattgccgt cgacgatgaa atcattcacc
     8701 gaacccagtt cgcgtttcca cataatctga ccactgcgca gatcaagcgc cgtcaggtta
     8761 ccattatagg ccagcgcgaa aacaacgccg ttaacaacga cgggagtcgt gtcaacatcg
     8821 ctcagacggt caatttcggt agaaccggtc gcctgggaaa tacgctgctg ccaaatcatc
     8881 tggccctgtt ccatcagcac tgcgctgacg cgaccattat cgccccccac gacggccgca
     8941 ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga gcgaaggcat atcaaggtta
     9001 actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt gtaactgacc gttactggtg
     9061 tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa gtgcttcacc cgcgacttta
     9121 gtttgccatg ccacagtacc atcgctggta ttcagcgcgt aaacctgcgc cttttcgctg
     9181 ccaatgtaga catgcccacc agacacggtc acaccgccag aaagtaatgc aggctcttta
     9241 gagaaccagc catctttctc ggccaggctg acagaccaga tttctttgcc atcatccgca
     9301 ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga caacgttgtc cgccagtgcc
     9361 ggatgaagat tggaatagaa gttgccaatg ccgctaccaa cggaagtact ccacgccgtg
     9421 gtcggcgtaa actggttttc aacggttggc aatggggaca tctttaccac atcttcttcg
     9481 ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa gcagtcctgg cagcagtaat
     9541 ttacgcaatt gcatcgggtc cctctcagat ggacaaatta ttaattttca tctgcatcat
     9601 ttcgctcagt gccggagtaa catcgctttt cacgcctgct tcccatgcac tacgcgcacc
     9661 ttgcttatca cctttgctca gcaatgcttc accacgcagg tcggcaacaa tggcagccca
     9721 cccttcacct ttgatggtat caagggtttt cagcgcggca tcagcctgct tgagctgtac
     9781 ctgaacgcga gcaagacgca gatttatcac ggctttgaga ttttcatcgc tcgtgtctgc
     9841 cagcccctgt tgtaactggg cggcagcttt ctctagttca tttttgtcaa caaattgctg
     9901 cgcaagttcc aaagaagcca gcgcaccata agtattttta ttttcagcag caaatttttc
     9961 cgccgccggg atgctatccg gtttgccttc gctcactgcg gtaaccgcat tttgataggc
    10021 aagagaagcg gagcgtgcag aatcaacctg atggctgttc cagtagcgcc agccaatcag
    10081 tgcgccaacg cccaaaatca ccccaacagc cagtgctttg ccattttcag caaaaaagcg
    10141 tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt tccacgctgt ccttctcctt
    10201 ccttaaccca gtaacgtgcg caagtgcgcg gctacgctat cctgcgcaac tgccgtttgc
    10261 tcaccagagc gcaaatcctt cactactgct gtgccgttag ccacttcaga ctcacccagc
    10321 accacagcaa cgcgggcacc ccatttatca gcacgggcaa actgtttctt aaagttgccg
    10381 ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat cacgcagacg ctcagctaat
    10441 gccatagccg cagattgtgt atcagcacct gaagccacca ggtatatatc gacaacagga
    10501 tcggctttaa attccggatt aacggcctgt actaacaata caagacgttc gaggcccatc
    10561 gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt ccacaagacc gtcataacga
    10621 ccgcctgcac acacggtgcc ctgggagccg agactgttag tcacccactc gaaaacggta
    10681 cggttatagt aatccagacc acgcaccaga cgctggttta cggtgtaagc gatccccgcg
    10741 ctctcaagca gtttgcacag accggcaaaa tgctcacgag attcctcgtc cagataatca
    10801 cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg gattttttga atccagcacg
    10861 cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt ccagcttttc tttatgctgc
    10921 tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg cttccagcga accgatagaa
    10981 ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc gccaccagcg ggcggtgagc
    11041 ataatcagtt cagcgtcgat atccggacct tgcagaccga aaacttcgca gcccaactga
    11101 tggaactgac gataacgccc tttctgcgga cgctcgtgac ggaacatcgg cccgatatac
    11161 cacagacgct gttcctgatt gtacagaaga ccatgctcga tgccggcgcg tacacagccc
    11221 gccgtccctt cagggcgcag agtcaggctg tcgccattgc gatcctcaaa ggtgtacatc
    11281 tctttttcaa ccacgtcggt gacttcacca atcgcacgtt tgaatagcgg ggtctgctct
    11341 acaatcggca agcggatttc actgtaaccg tagctgccga gcacgttttt cagtgtgcct
    11401 tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat cgttcatgcc gcgaatggct
    11461 tgaatgtttt ttgccacgtt tattctcttt ctgaatataa aaatgaaccc tcaacgcttc
    11521 cctcaatgtt tcgggagcta tgcgggttca atcatacacg ggaagcgagg cgcttcccat
    11581 cacgttatta tttttcaacc tgctgaacgt caattcgacg cgcttcgtcc agctgactgg
    11641 ctttcgcacg aatgcgtgct tccagctggt cgatcatatc gttgttgtcc agacggtctt
    11701 tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc gccggtgacg ccgagtgtag
    11761 aaaccagcgc ctcacctggg ccattcacca cgcagccgat aatcgaaacg tccatcggag
    11821 tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt accgataaca tcaaattcct
    11881 gacgcgaaca ggtcgggcag gcgatgaagt tgatcccgcg cgaacggata cgcagcgatt
    11941 tcaaaatatc gaaaccgact ttgatctctt cgaccggatc ggccgccagt gatacgcgca
    12001 gcgtgtcgcc gatgccttca gacagcagca gacctaaacc aatggcggat tttactgccc
    12061 cgctgcgtgc accaccggct tcggtgatcc ccagatgcag cggctgatcg atctgttttg
    12121 ccagcaaacg ataagactca acagcgagga agacgtcaga cgctttcacg ctgactttga
    12181 actgatcgaa gttcaggcga tcgagatgat caacatggcg catggcggat tccagcaacg
    12241 cctgcggcgt cggttcgcca tacttttctt gcagatcttt ttccagcgat ccggcgttaa
    12301 cgccaatacg gatcggaatg tttttatcgc gcgcacagtc aaccaccatg cgaatacgct
    12361 cttcattacc gatattgcca gggttaatac gcagacaatc gacgccgtat tccgctactt
    12421 tcagtgcaat gcgatagtcg aagtggatgt cagccaccag cggcacgtta acctgctgtt
    12481 tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac ggagacacgg acgatatcag
    12541 cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc ttcgacgtct gtcgtacgcg
    12601 tattggtcat ggactgtacg gcgatgggag caccatcgcc aatcggcaca ttcccaacgt
    12661 aaatacgtgt tgattttcta cgttgaattg gagcctggtt atgcatgaaa aatctcccgc
    12721 gttacccgtc tgttactgcg ccggtgattg ttcggcattg agggtcagac gcgcaacctg
    12781 gttagttctg ataaaacgac tcagatcgac aggtttccct tgatactgga tctgtactgc
    12841 ggctggcgca ccaattttca gtttgtatgg tgcctggccg gttaagttca aattaccgtc
    12901 tttacgctgc ataccgctaa acaatttttt accggtagca tcagtaacct ccagccagca
    12961 atcggcagta aagttcatca ccagcgcatt cggatcagcc gccggcgtag tcacgcctgc
    13021 ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt gttgccgttg gtgccggggt
    13081 cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc gcattctgtt gcggatcaac
    13141 agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg gttgcggtag tatccacaga
    13201 cgctggcggc gtggacgttg tagccgggtc tgtagttgtc gacgtattta acggaacact
    13261 ctgcccctgc tcgctattac tgctcagttc cgccgaagat tgatcggcca tagtggtgat
    13321 ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca ccgctcaggc cgataaccac
    13381 aaacaacacc agccaggtga aggtcatcag ccagccgtca cgttttttgc ggcgtttacc
    13441 gagggaaaaa ctctgcatag gcgcaacttt tgcagcccgt agtggagcct gcttttccag
    13501 ccctggcagc agttcttctt ctggaatatg taccagacgc gcataagagc ggatatatcc
    13561 gcgcaggaat gttgaagcaa gatcggcggg tgccttatct tcttcaatgt cgcgtaccgt
    13621 ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga ctaagtccta gttgttcgcg
    13681 agcattacgc aggcgagcgc cggtagtaag tgcttcattt tggtcgtgcg tggcttcagt
    13741 attcattcgc tgcaggtacg tttaaatgag aattgagatg ccggtgaacc atcatgctca
    13801 cccacaccgc gaaacatccg ttaagttaac cgttatcata cagtataaga ccgtctgtgt
    13861 gctcgtgaca aagcccacat tcactaaggc taacttactg ttgcatcgtt acatactgcc
    13921 ttaaagtcag caaaaacgca ccgttaatat tgaccagaca attgcaactt aatacttcat
    13981 tcataaaaga ttacgccacg gtacataaag taaccgtggc gtaatggcta tcagaccgct
    14041 ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg tacgtttcgt acggtcgata
    14101 acatcgcccg ccaactgacc acaggcagca tcgatatcat caccacgggt tttacgcaca
    14161 atagtggtga aaccgtagct catcagcact tttgagaaac ggtcgatacg gctgttcgag
    14221 ctgcgtccat acggcgcgcc cgggaagggg ttccacggga tcaggttgat cttacacggc
    14281 gtatctttca gcagttccgc cagttggtgc gcgtgttcag tgccgtcgtt aacgtggtca
    14341 agcatcacat actcaatagt gacacggccc tgattggcgt tggatttctc cagataacgg
    14401 cgcaccgcag caaggaacgt ttcgatattg tactttttgt tgatcggcac aatttcgtca
    14461 cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg caacgtcgat catatcgccc
    14521 agtttatcca gcgccggaac tacaccggaa gtagaaagcg tgacgcgacg tttagacagg
    14581 ccaaaaccga aatcatcaag catgatttcc atcgccggaa cgacgttgtt caggttgagc
    14641 agcggctcgc ccatgcccat catcactacg ttagtgattg ggcgctgacc ggtgactttt
    14701 gctgcgccga cgattttcgc cgcacgccac acctggccaa taatttccga tacccgcagg
    14761 ttgcggttaa agccctgctg ggcggtggaa cagaatttac actccagcgc acaccccacc
    14821 tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga tatacaccgt ttcgacgcgc
    14881 tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg atgaacgctg ttcttcaacc
    14941 acttccggtg cgcggatttc cgccacctct ttcagtttgc cgcgcaacac tttgttgatg
    15001 tcggtcatct catcaaagtt gtcgcagcaa tagtgataca tccacttcat cacctgatcg
    15061 gcgcggaagg gtttttcacc taaatcttta aaaaactccc gcatctgctg acggttgaga
    15121 tccagcaggt tgatttttcc atctttcgtg gtgacgtttt caggtgtgac taattgttca
    15181 gacatatgct attccggcct cgttattaca cgttatggcc cctggaggat tgaaaaaaga
    15241 aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat tgtacaaatt ctggcgcacg
    15301 gatgccacgt ttgcacgcgg catttacgaa attattaacg ggtgcgcggg cacacttcgc
    15361 cttcgccaaa gaaataagcg atttcgcgag cggcagattc gacggaatca gaaccgtggg
    15421 taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag agtaccagcc agtgcgtttg
    15481 ccggattggt cgcgcccagc agatcgcggt gacgctgaac ggcgttttca ccttccagca
    15541 cggaaaccac gatcgggcca gaggtcatga attcaaccag accatcaaag aacggttttc
    15601 catcgtgttc agcataaaag ccacgtgcct gttcaacggt caggtgcagc attttggtgc
    15661 cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat attaccaatg acgttttttg
    15721 ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat agccattttt acctctgtaa
    15781 attgttctgt tgttgtctgt accagcgtac gagatggcgc ggattataat gagcaacagg
    15841 gccgttgact attgatgaag gtaacatttt tttaaaataa aatgagtttt agcaacaatc
    15901 agggacaaaa cagactattg catgacaaat ttcactgtcg cgatttgccc cacatcatcc
    15961 atcaccagca attgataatc acctttatcc gtcaaatgca gggtcacgtt gcgcccgcgt
    16021 tcagttaacg gttcgccatt cagaaaccac cagcgttcac ctgcccctcc acttgattgc
    16081 aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa taatcgcgcc atcgcgaacg
    16141 cctgtcagct gcagagggag ctgggcatcg tgaccgtatg acgggcaaat tgtcgaggcc
    16201 ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc agggttccag cggtagcggc
    16261 cagacattta tcatttcttg tcgcgcttgc gggcaatcag cggcaacacg tttgccattt
    16321 tcatccagcc agatggggaa acgaatgcca ttaatgcctt cctgatccgg cagtaataga
    16381 gttggcggct gactcccgtc cagcagccag gttgccaggc ggcggcgaca gttaccgtca
    16441 ccttccggca aagactgtcc gcccggccag cagataacgc cacgagtgac tgagttcggg
    16501 cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga tattattgac ctgattcaac
    16561 aatggtacgg cactggcaaa gccaaactga ccaacaacgg gcgtgccgtc cggtctgcca
    16621 gtccagatcc caatgacata gcgagcgtta accccaatcg cccaggcgtc acgatagcca
    16681 tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg gcaaggcact atccggcaag
    16741 ggtttcgctt catcagccat aatccggcga atgatccacg ccgcccccga cgacattaaa
    16801 ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt tgcctgcctt gccgtggcga
    16861 gcaaacgcgg tatacgctgc cgccatatct tccagttttg caccagcgcc gccgagaatg
    16921 agtgaaagat tcggcgcagc accgttgggc aaatataacg gcaatccaac attgcgtaac
    16981 tttgccgcaa accgtttcgg tccataggct tccagcacct gcacagcagg taagttcagc
    17041 gagcgcacca gcgcctcgct catgctgatc gggccatgaa aaccgctatc aaagttacct
    17101 ggtcgataat caccggtgcg ccgggggacg tcttgcagca gtgatgccgg gtggatcaag
    17161 ccttcatcca gcgccagacc ataaacaaac ggtttgagca ccgatcctgg cgatcggatc
    17221 gcattgacca tatcaacatg accaaagcgt gaatcatcgt tgagatcaac cgatcccacc
    17281 cagccgcgaa cacgcatatc ggtatgatca accacgatca tcgccagtga gctgcgcggt
    17341 ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac gtcgttgaag accggcatcc
    17401 agcgtagtaa cgattttgtc gcttttgctt ttaccgagca tcatgcgcga aaacagcggt
    17461 gccagttgcg gcatttgtcg gggtgccagc cagatgggtt cttcccttga ctcttttacc
    17521 tgctcacggg accacacacc ttgcacggcc atccgttcga gcactttatt acgcgcggct
    17581 tcggcacgct ccggccaacg atccgggcga agacggctgg gcgcttgcgg caaaaccgcc
    17641 agcattgccg cctcggaata gcttaaattc gcaggcgact ttccgagata agcccaactt
    17701 gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc ggttaagata caaggtcaga
    17761 atttcacgct tagacagatg ccattccagt tgcaacgcgc gccagagctg gcgaattttg
    17821 ccgccaaatg ttttggggtg aggatcaagc agacgagcaa cctgcatagt gagcgtgctg
    17881 ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg ctgcgcgcgc caccgagaat
    17941 ggattcaccc ccggatgctt ccagaaccag cgatcttcat aattgatcag cgcttcaagg
    18001 taacgtggag aaacatcttc gattgttacc ggataacgcc agatgccgtc agcatcggcg
    18061 aagcgccata gcggcgtacc atcctgcgcc acgaccactc gtgcgggatt gacttcatgc
    18121 agcggtagag gccagagttt atctgctccc cacgctgcaa gaaaaaggag aaatggcgca
    18181 gctgccagcg ttatccagca gccgcgtttg gttaacaagc gaggcattta cggtcggaca
    18241 atcagcagat cttcagccgc gccggtcgcc cgccattggg gaacatacat tgattccacc
    18301 atcggttgcg gtacctgata cgttcccggc gtcaccgccc gcgccagata caccagcgtt
    18361 accggttggt attcatcaac ggcaaccgcc gccacaaagc gatcgtcacg gaactcaatg
    18421 tgcttaatac tcgcctgctg catctggttc agtaagtttt gcacttcgcc accactttgc
    18481 tccaggctgg cgctaccgtt cgccagattc tgattttcca gttccagacc cgcaggcagc
    18541 agatccacga ctaacgcatc cggcacgctg ttactggctt ttacctgcaa ccagaccagc
    18601 accagatcgc cgctacgtaa cgagtccagc gatttgctct taccatcagt accaagaata
    18661 tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg cggattgcgg ataaccgctg
    18721 gcatccacac gcagccataa cggctgatcg ccactgtttc tcacctgcaa ggtggcaagt
    18781 tgatcgctat tcagattgct gtttagcgtt ttctcgcctg tcagcggctc agctgagaaa
    18841 gaggtttgcg cctgccattt acccggtaaa tcctgaatcg tacgggcagc caggaacaac
    18901 gcgttacttt cctgcgtcga tagccagcgt tcaccaaatg cctgctgcga aagcgtgttc
    18961 agcaaagtgt tctgctcatc gggtagcagt ttattttctt ccagcaagta gagcattaac
    19021 gcgttgtcgc gcagtggact accgtaatca cccagccata tccgctcatc actatttctc
    19081 ggcgttttca gcgccagcac aatcgcttct tcaccacgcg ttgcatcacc catggttttc
    19141 agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg catctgcacg atgctcccag
    19201 atttcacgca gcgcacccag tggtgctttt tgctgacggg ccagcaccag cgcagcgtaa
    19261 gactgaacgg cgaatttact ggctttgaga ttatccgcgt acgggatcga catcatgccc
    19321 ggatcttgta aatagcgcag cagacgctca ttaccccggt taatggcgtc tgtcggcacg
    19381 ctgtaaccct gttcgcctgc gcggaccagg aaatccatca cgtaagccgt cagccagtac
    19441 tcttcgtcac cgtttttatc ccacagcgca aagccgccgt tatcacgttg catttgcagc
    19501 aaacgggaaa tgccgatatc gaccgatgca cggcgtttct catcactgtc gcctttgatg
    19561 cccaacgcct gcagttgggc tgcgttggta taaagtgacg ggaacaggcc gctggcggtt
    19621 tgctcaagac agccgtacgg atacgctttt aactctttga tataacgtgc aatgttcagc
    19681 ggtggtttgc cgctcaacaa caattgccct tccagcgtaa caggcgagaa gttttgcaat
    19741 ccatccgccg gaatcgccca tgtctcacca ggctgtaacg ccgtaccgta attaaccgtt
    19801 tgtgccggga acgccggacg gacgccgatt ttccactgct tatgctgatc ggcaacggtt
    19861 tcacccggta acgctaaccc gctaatggtg gcctgaattt ctccatcgcc ataacccggc
    19921 aatgctcgca ccgggataaa cagcgtagta cgcacacctg gcgctaattc aacggctgcg
    19981 ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca gggcaacgtt cagtttttgc
    20041 ggtttatcgg taagattagt gatatccagc gtcagacgcg aggtatcgcc actcgccata
    20101 aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa ctatcacttt gctttcgttg
    20161 ctaccgaaat catctgccgt ccaggcttgc gccatgacgc gcaattcacc gttaaaatcg
    20221 ccaatcggca gtgtaaccga gccttcgccc tgttcgttga gcgttaccgg cagcgcctgc
    20281 tgcgcgacaa tattgacgtg attgaccggc ggtttaccac cacgtttcag ctcatcacca
    20341 tcgccaccga aacgcagagc tgccagacgc ccctgacctt caataacctg accgtaaata
    20401 tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg cctgccacgg atctggcgtg
    20461 acgtagtcag taatattcag cacaccacta tcaacggcag acaccagcac attcacctgt
    20521 ttaggcttct cgccattttt agtgctggct ttaattttca cggttaatgg ttgattggga
    20581 cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac ggttttcatc gccaagcggc
    20641 agatgcaaca caccaaccgc gcgttttggc gtcgcggagc gagatttatc gccaggacgt
    20701 accaccagcg tacttaaata cagatcatga cgattccagg ttttatcgac cggaatcgtc
    20761 agatccagcc cttgagccgg aacatcaatc tcttgccacc acagcggccc ttcactggac
    20821 tcgaccatcg cataaccttt acccgccgtt ggcgcggcga tatgcaactt aatggtgtcg
    20881 ccagggcgat aactggcttt atccagtttc agcgtgacac ggtcgggtcg cactgcgccg
    20941 ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga aacgaacact actgaccgct
    21001 tcattcggcg ctttgacttc cagacgataa gcgccccact ctaccggaaa actgaccttg
    21061 ccggtttcgt ccgctttcag atccagagtt tgttcatttt cgatcagatc tttttgatca
    21121 aactgtgact gccagccttc atcttctgac cagttccagt agtaatcgcg gcgttcgcga
    21181 atcaggcgca cctgcaagcc cgacacggct tttttcacgc cttgcgcatc gctataaacg
    21241 atgtcaaaag cggcgttact gccttcatca acaatgggct gtttcaccgt gctgtcagta
    21301 cgataatcgt aaaccgattt cgaggcgaac tgcggacgga tccccggcaa tgcatcggca
    21361 ggccagatag cctgctcagc gcggcgcgtc accgggcgac cgcccgattc cagcaaacta
    21421 ccctggaaaa taacctgtaa tggggaatgc gtttccttcc actggctttc tgtagaaact
    21481 tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat ccagcgtgcg ggaaagattt
    21541 tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg acacagcttc acgcagtggg
    21601 cgcaggaaaa gttgcccttg caaagtatta ccattagcag gtgcaccata caggtagtac
    21661 cccaccacgg agaatttcac ttcatctttc ggcgttagcg gggttttctc accggtcaga
    21721 ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga aatcccacat ccgatactgg
    21781 ttatcgcccg tgttagcgcg aatatgccac ataccggttg ccgcattgct atcgagtggc
    21841 caggtaaagt ggtagaggcc attctccggc tgactaacga cgctcctgag tacctgccca
    21901 tcgggtttaa tcacgtctaa cttgatgggt tgattgggca acgctttacc gtctgcatca
    21961 cgcagcaaac cattgaggat taccgtttca cctgggcgat aaagatcgcg tgggccaaac
    22021 atgaaaaact gtttgctata gcctggcgcg ccagcaatgt taaattctgc taagtccagc
    22081 gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt tacgcgccaa cagtaatgcc
    22141 gcgtttttat cattttccag ctgcacatgc ccctgagcgt cactggttgc ctgagtcaga
    22201 gtctgccctt tctcatttaa taaagagact tcaattcctt gctgggccgc gccgttttcc
    22261 agactttggg taaagatatc cagacgattg tgataacggt gagctgaaac gccgatatca
    22321 cttaacgtaa acagcgtcgc gggattactg taatcgtaac gtccagcctg attcatcaca
    22381 gccagataca cgcccgcctg ctgaagcggt ttgatatcgc ccagcggcag caataatttt
    22441 tcacgggtgt tacgcgcagg attgagatca aaccgtccgg tgtagaccag atccgccatc
    22501 tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc ggtattccca ttggctaatg
    22561 aatgctggca gagattctgg cttaacgcgg aagaagttaa catcgacatt attaacgttg
    22621 agcgccatta ccggcagccc ttcaacgact ttgccaggca gcagcgaacc acggctggca
    22681 aaaccgacgc tgggttggat gtcgcgggta gttatagttt tttcgtaatc tttactgaag
    22741 gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa cgatcaaatc acgtttcggt
    22801 tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca gctcccaggc accatccact
    22861 ttgccgcttt ttttatcgac gacatgaata acgcgtgaga aatcctgatc cgggtcgaga
    22921 gggatggaga acgtcagcac cagcgtggct gcaccatcaa gttggacttc agagagatcc
    22981 agcaatgtca gcaccttacc ggcactctgt tgggccagtt tttgtctctc cggcacggag
    23041 agttttgctg aactcgcgtt ttctgaagag gccgctttag taacttcaga aggcgcatct
    23101 tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc ctgccagcgc cagcattagc
    23161 atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct gccacaatgg cccgttagca
    23221 acgtcgaata attattatgc gtgagaattt acgttatgta attcagtatc acgcccatac
    23281 aatccgaatt ttagtgagaa ttgtggctca gacagccaaa tcagcctctt tcgcttgtcg
    23341 cttttcacta aacatccgac aatttagcct aaccccggca aaaatggaga tgcctatgtc
    23401 cacgacatgg tttgtaggag ccgactggct cgccgaacat attgatgacc cggaaattca
    23461 gattattgat gcccgcatgg cgtcgcctgg acaggaagat cgtaacgttg ctcaggagta
    23521 tctgaatgga catattcccg gcgcagtgtt ttttgatatc gaagcgcttt ctgatcacac
    23581 ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc gccgtggcga tgcgtgaatt
    23641 aggcgttaac caggataagc acctgattgt ctatgacgaa ggtaatcttt tctcagcccc
    23701 acgagcatgg tggatgctgc gcacctttgg tgtagagaaa gtgtcgattc tggggggtgg
    23761 acttgcaggc tggcagcgcg atgatctgct gttagaagaa ggtgcagtag agctgccgga
    23821 aggagagttt aacgccgcgt ttaatcctga agccgtggtg aaagtaaccg atgtattatt
    23881 ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac gttttaacgc
    23941 agaagttgat gaacctcgcc caggtttacg tcgcggacat attcccggag cactgaatgt
    24001 tccgtggacg gaactggtgc gcgaaggcga actaaaaacg accgatgaac tggatgcgat
    24061 attttttggt cgcggcgtca gctacgacaa accaattatc gtcagctgcg gctctggtgt
    24121 aacggcagcc gtggttttgt tagcactcgc gacgctggat gtgccaaacg tgaaactgta
    24181 cgacggcgca tggagtgaat ggggcgcgcg ggcagattta ccggttgagc cagtgaaata
    24241 agtattttac aggcaataaa aaaccgccga atttggcggt tttttattgc tggtctggtt
    24301 cgcggccttt ccagcaggtt gacttgtgtt acatgagcaa cgcaggtgct tcacagcaaa
    24361 acaatactca ccagtaactc tctttttgtc aagcaaaaga gagtaattat tgtttattta
    24421 gcgtattatc gacaccggcc ctttccgccg tgttcggtaa taaattaacc tggcttatta
    24481 gtccgaattc agacaaatat aaacaaatcc tgctcaaaat taaaaatact aaccggtaaa
    24541 agatattact taaacatgta aattcacttt cctttaaaaa acaaaaaacc gccaaaatca
    24601 ggcggttatt tgttgctggt ccggttcgcg gcctttccag caggttgtat taccgtagta
    24661 atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa
    24721 gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa
    24781 gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc
    24841 ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca
    24901 caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag
    24961 taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca
    25021 gcaagtaagc ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttctgttt
    25081 aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat gttcggtaat
    25141 aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc
    25201 aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc
    25261 ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat
    25321 aaaagcacgc ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg
    25381 tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg
    25441 gctgctcagc ggatttccct ctccaccaat caacaaactg atttcacgcg gcataaattc
    25501 tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa
    25561 tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc
    25621 taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc
    25681 aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt
    25741 ggattccagt agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc
    25801 cagcaggtct tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt
    25861 cggggcgcaa atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt
    25921 gaatttgcag aatttgtagg ccggataagg catttacgcc gcatccggca tattagttta
    25981 cgccgttaac agattagcaa tcgtgcgcac accaagtccc gtagcacccg cagaccactg
    26041 ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata
    26101 gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc
    26161 gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc
    26221 cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc
    26281 aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc
    26341 cccggtgagg gtcgccgcat caatgatcag ttccggtttc tgcgcactgg catcaatcag
    26401 accatcggca agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc
    26461 gttgcgatag gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc
    26521 acagcagagg aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc
    26581 cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg
    26641 tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac
    26701 tggcgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc
    26761 tgaaccgcgt ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt
    26821 ggtgatccga taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg
    26881 tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc
    26941 gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc
    27001 cactttacgc gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc
    27061 atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc
    27121 cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt
    27181 attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt
    27241 aatcttcatc gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca
    27301 gccccaggat ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc
    27361 ccaggagcat agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg
    27421 tgaagcatga cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag
    27481 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
    27541 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
    27601 ttcgccaatt tcgcggctat cggtccattt aagtcccata ctaacctctg ttaatgctca
    27661 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
    27721 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
    27781 tcctgctctg agctttccgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg
    27841 gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg
    27901 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gagatcctga
    27961 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
    28021 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
    28081 tttaatcgct tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc
    28141 ggcagcatcg tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag
    28201 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg
    28261 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
    28321 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
    28381 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
    28441 accaccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
    28501 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
    28561 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
    28621 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
    28681 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
    28741 gacgacttta tccgggtcga tggaagtcag aggtggacga ccgaaaaatt cgcctacccg
    28801 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcatc
    28861 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
    28921 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
    28981 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag
    29041 ttcacgctga acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc
    29101 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
    29161 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
    29221 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt
    29281 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
    29341 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
    29401 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
    29461 cgggttcagc agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
    29521 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
    29581 tttaacagaa ctaatggtgt tggcggtatc aagcgctgcg ttagtacgcg cgtcataacc
    29641 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
    29701 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
    29761 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
    29821 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
    29881 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg
    29941 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
    30001 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
    30061 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
    30121 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
    30181 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
    30241 gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
    30301 aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
    30361 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
    30421 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
    30481 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
    30541 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
    30601 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
    30661 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
    30721 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
    30781 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
    30841 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
    30901 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
    30961 tcacccattc ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa
    31021 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc
    31081 cacaggccgg tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac
    31141 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
    31201 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
    31261 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
    31321 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
    31381 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
    31441 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
    31501 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
    31561 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
    31621 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca
    31681 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
    31741 cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac
    31801 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
    31861 ggtcgatagg cagtttaccc acgctctggg ttgcatcaac gtgatagata atgccacgag
    31921 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
    31981 gcatgatgga aacaaggatg gtgtcgtcac gcatcgctgc ttcaagttct ttcaggtcga
    32041 taatgccgtt acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac
    32101 ggcaggtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
    32161 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
    32221 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
    32281 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
    32341 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
    32401 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
    32461 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
    32521 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
    32581 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    32641 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    32701 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
    32761 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    32821 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    32881 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    32941 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    33001 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    33061 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    33121 cccgagtaaa ttggtcaact atttacttga ctgatttact cgggtattta accttcagtg
    33181 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
    33241 cctacggttc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacta
    33301 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
    33361 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
    33421 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
    33481 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
    33541 ctcgccgttt tcctgagttg ccagccaggc catgcgcact tcataggcaa taacctgaac
    33601 cgccatcgcc aggttcagcg agctgtattc cgggttagcc gcaatcgcga catgataatg
    33661 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
    33721 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
    33781 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
    33841 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgctgccag
    33901 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    33961 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    34021 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    34081 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    34141 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    34201 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    34261 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    34321 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    34381 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    34441 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    34501 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc
    34561 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    34621 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    34681 caaactgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    34741 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    34801 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    34861 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccacgtgttg tgaaagccat
    34921 gctggcgaac atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt
    34981 gatgtcactc acccgccctc gcctttcagg cgctattctg aaatacttcc tcaccgcttt
    35041 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    35101 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    35161 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    35221 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    35281 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    35341 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    35401 ggtcagttgg ttacccgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    35461 taaatcaaaa ggcacgccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    35521 tgtggtgcgc catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat
    35581 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    35641 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    35701 tggctactta cttgatgaga gttacagcgg cgaaaattat atcgccagcg tcagcccgat
    35761 cccgctttta ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa
    35821 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    35881 tgatgctttt tccgatcgcc acggcgatgt ttatcgcgaa cagatggtgg actttatcct
    35941 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    36001 caccttaaga catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac
    36061 gcttgccaac atttctgatg attagcattc ccttcgtcat ttccctgagc aaactttagc
    36121 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    36181 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    36241 tgaccgccac cacggcaact ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    36301 ttgatatcag cgaaacagga caggagatcc tgcgctatca tcaactcaca ctgacggctg
    36361 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    36421 actggctacg acgtggtttc cgtctgtgtc catcatttat taccagccat ttcacccccg
    36481 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttatgac gataccaatc
    36541 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa acgcgcgata
    36601 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca acaccacgct ggaattacgc
    36661 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    36721 aggaaattgg acgccactgg cagcgtcggg cgctccagcc tgtaccacct gatgagccac
    36781 tgtttatggc actacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    36841 agcgagacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    36901 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatacc cacctcgccc
    36961 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    37021 ccagactgta cccacgcctg gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg
    37081 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    37141 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tcttcttacc gggaatgata
    37201 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    37261 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    37321 ttattgcgcc ttataccatg ccattaccgc tcttttcacc accgctgatc tatacggacc
    37381 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    37441 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    37501 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    37561 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgagca
    37621 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    37681 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    37741 caaatcgccg agatcaacag catatcgcgt gcactacaac ggcggaaaag tttattactc
    37801 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    37861 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    37921 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    37981 gctgaccaac cggtgctctc ctgctggcgg ctttcccctt gtggctgaat cgtcggacgg
    38041 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcgcgag gatcacccga
    38101 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    38161 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    38221 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    38281 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc
    38341 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    38401 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    38461 aaaccaatcc ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag
    38521 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    38581 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    38641 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    38701 acgcatgttg caaaaataat gcgtaattat gccgttacgt ttgccaaacg ttcctgcaca
    38761 atggcgatga aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa
    38821 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgtccggt attaaaatta
    38881 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    38941 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    39001 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    39061 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacaca
    39121 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    39181 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    39241 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    39301 ggcagtaaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    39361 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    39421 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    39481 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    39541 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    39601 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    39661 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    39721 tcaatacatc tgatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    39781 accaactgat tgatacccaa aatggtcggg tcactccgcg tatttatacc gacccagata
    39841 tttaccaact ggagcttgaa cgtattttcg gccgttgctg gctatttctc gcccacgaaa
    39901 gccagatccc aaaacctggt gatttcttta acacctacat gggggaagat gcggttgtcg
    39961 tagtgcgtca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    40021 tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct
    40081 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    40141 aagggttgtg taaatcccac tggggactaa acgaagttcc ttgtgtggag agttataaag
    40201 ggctgatttt tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca
    40261 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    40321 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    40381 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    40441 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    40501 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    40561 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    40621 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatattt
    40681 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    40741 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    40801 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    40861 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    40921 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    40981 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    41041 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    41101 aggtgatgaa atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct
    41161 ctttcacgaa gccagcttac tggacgactg gaaatttcgt gactggctgg cgcagctcga
    41221 cgaagagatt cgttacacca tgcgcaccac agttaacgcg caaacacgcg accgccgcaa
    41281 aggcgtccag ccaccgacaa cctggatttt taatgacacc aaagaccagc tggagcggcg
    41341 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcacccgtca
    41401 cttaatcagc aactgccaga taagcgaaac cgacatccca aacgtatttg ctgtgcgggt
    41461 aaattatctg ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    41521 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    41581 cctggatcaa gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    41641 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc
    41701 gtcatcgccc tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    41761 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    41821 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    41881 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    41941 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    42001 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca ggttgccacg ctggaactgt
    42061 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    42121 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    42181 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    42241 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    42301 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    42361 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccgcca
    42421 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    42481 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    42541 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    42601 cgctgcaatt tttcccgcaa ccggcggatt ttacgggtcc gtatgtgatg ttggcatcgc
    42661 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    42721 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    42781 ccatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    42841 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    42901 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    42961 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    43021 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    43081 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    43141 gctttaccct acgccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    43201 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    43261 agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac
    43321 caccgcccgt gcaacgctat cttttacagc gccaccagca ggctggtgtg cgcattctgc
    43381 tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg
    43441 gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac
    43501 tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca
    43561 cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg
    43621 cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg
    43681 caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca
    43741 gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc
    43801 cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcagtca
    43861 cgctgaatca ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt
    43921 ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa ccaggataat
    43981 tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat
    44041 cacataatct tgcaaaggga ttggttatga acacactacg ttattttgat tttggagctg
    44101 cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc attatttttg
    44161 gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg ttgggcgcgc
    44221 cgatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc gcgatattaa
    44281 tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac acgctgggta
    44341 cggcggtgat tggtcaccat tactaggata tgaccggcga tgcggttggg ccaaatatga
    44401 ttaatttctg gaagaatgtc agtatcgctg gcgcattcct gctgttggca attaccggac
    44461 cgggggcgat ttctctcgat cggcgttagg aaagatgccg gatgcggcat gaacgcctta
    44521 tccggcattt tataaattac agcaacgcaa cccgcaacga catctctgaa gttgttgatt
    44581 cacctggtgc aagcgcaatg agatcacccc cttccgggcg atgatgatca tccggcgcat
    44641 ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg
    44701 aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt
    44761 cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg ttcacccact
    44821 ggcgcggcaa cggcgcgggc tggttaaaat ccagttcctg cggtagctgc tcgcaaaact
    44881 cacccgccag ccactgctcc cgctccagcc agtaaccgct cgcctgtgcc tgaatccgcg
    44941 tttgcggcga caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc
    45001 cttgattggt gacagagagc gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac
    45061 ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa ctatcatcgc
    45121 tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc
    45181 actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacga ttgccgctca
    45241 cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca ccgctttttt
    45301 taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt
    45361 cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc cgctaactcc
    45421 ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca gcgcataggc
    45481 atcacctgcc tgttccagcg agaagcgatg ggtaatggcg ttacgcggcc acagcttcca
    45541 gtccgtcaga tcatgggcgc atttttccat atggaacaga ctggtcaccc aggagccgat
    45601 aatccgccgt tgatggtgca tcagatcggc gctgacctcg aattccactt ttccggtttc
    45661 accaatgtaa accacccgtc cccagtcagc ggtggattgc agtgccagca agcgacctgc
    45721 ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtgagtt cggcgataat
    45781 ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc atcaccccta actgttttgc
    45841 catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac cgcgaccttt
    45901 cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca cgttatcgct
    45961 gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc aactgataaa
    46021 cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt cttccgccag
    46081 cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt
    46141 acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata
    46201 caccagcacg cgatcaccct ctttaaaatg gcgacagcct tgccccatcg ccacaatctg
    46261 cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg gtgccgccgc
    46321 cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc cggaggattt
    46381 cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc gcagatcgac
    46441 ggttgaattt cctggtaaat aagctgccag catcgttttc ataaatccct cattaacaat
    46501 acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct
    46561 ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat gatgacgccg
    46621 cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt accgattagc
    46681 gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc
    46741 gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg
    46801 ttaccagaac cgaggcgcgc cgccaacaaa atgccggtca ccgccgctaa taatcccgaa
    46861 agggtaaaga taagaatgcg cacccgacga acgttgatgc cgcacaactg cgccgccgta
    46921 gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg ttttgcggct gatgaacaca
    46981 aacagcgcaa acaacacaat catgatcagc gcggataccg gcacaccgag aaattgtccg
    47041 cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt cgtcataaac
    47101 agccccattc cgcgcagggc gctccacagc cccagtgtgg caacgaaact tggtacgtta
    47161 aacacgccgc gcagcacccc ggccagcgtt cccatcagcg cgcctaacag caacaccagc
    47221 agacacgcca ccgccagcgg aacttcaaat tgcagcaaaa atgccaggca caccgagaca
    47281 aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat cagcgtcatc
    47341 gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg gttattcaat
    47401 gagataaagc caggcgcatt cagggagaag accagataaa gaatggcgat taccaccagc
    47461 aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct cttgccctgc
    47521 ggcaatgata atgacgaagc agacatcttc aggttcctcg gtaaaattca tcagtgcaca
    47581 gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac
    47641 gtaccgtgct gtaacaacag gatgcggtcg cacaccagcg gcaactcctc cacttcactg
    47701 gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg gtaaatctgc
    47761 tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg
    47821 gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag cgtgccgatg
    47881 ggcgtttcgc tactggcggc cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg
    47941 cggatggtgg accattgcag cactccgttg gcgctgattt tttgccgatt ggtcagcact
    48001 gtattttcgt caacgcccaa ccagggaatg atccccgctt ctttgcggtt ttctggcgta
    48061 tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcgtgatttt ctcgccgttg
    48121 ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag caattcactg
    48181 cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cgcgacgtaa cgtaaagctg
    48241 atatcctcca gcttgggctt atggcgtaac gcacggactt ccagtacggc ctgatccaca
    48301 atttcctgag gggcaaccgg cgcaatatca acgtgatcac gccctagcat tagcgacaca
    48361 atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc atcgcgcata
    48421 acggtggcac aggaggcaat gcggcggatt tcttccatcc ggtggctgac ataaatcacc
    48481 gccacgccca gtgccgacat ctttttcacc gcactgatca ccagttcaac ttctgcactg
    48541 gcaagcgagc tggtgggttc atcaagaatg accacgcgcg gctcgccctt catcacccgt
    48601 gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg ttcaggacta
    48661 acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg caggtaatca
    48721 atcatgccgt tgcggcgagg ccattgacca aggcagaggt tttctgccac cgtcagccct
    48781 tccaccagac ttaattcctg ataaaccgca cgaaccccca gttcagcggc acggcgagtc
    48841 aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc gctatccggg
    48901 cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt tttacctaac
    48961 agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc aacgacgccg
    49021 gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt tgccgtaaac
    49081 atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg ccagccactg
    49141 tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc
    49201 ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc
    49261 ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag caatttcggt
    49321 tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt
    49381 taccgcgccg agtgtcgcac cgcccgactc ccccataatg gcgttgagat ccggcgtgga
    49441 gataatcagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctgattagc
    49501 gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg
    49561 cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt aaattttatt
    49621 ggcaataaaa taatcggcgg cagcgttacc cagtttttta ccaaattcca gtggatcgcc
    49681 gaccagatac gccgagacat atttatcgac acccttttga ttaatacagg tgttgtagca
    49741 aatcaccgga atacccgctt cactggcgcg acgaacggta cggctactgc cattttcaga
    49801 cactgccgat aaaataatgg catcgacatt acgcgccacg agggtatcaa caaaggtact
    49861 ttctttcgaa atatcaccct gggcgttagt ttcaattaac tgtacctgta ctgaagaatc
    49921 ttttgccgca tcctgaacgc cctgacgcac tccagcgtaa tatccctggg tatcaaggta
    49981 tattgcacca atggtcattt ctttatccgc agccctggca aatagtgcgc tacctaatag
    50041 cgtagccatt aataatagat tacgggttgt tctcattttt ttaggcatag tgcctcctgt
    50101 agggttttta ttaacaacgg cttattctaa ttattttgtg atgagcggca gcgcgtgccg
    50161 ctcaaatatt acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacggt
    50221 ggtgaataat gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga
    50281 ttcatattgc gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt
    50341 ttctttatgc tgctgttgta aatcgccata cagcgacaac aggtcaggtt gtgaaacagc
    50401 aaagaaatcc gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact
    50461 aaacagttgc tgtgcgattt gttgttcgcc cagcagtcgc agcgccattc cttgccagaa
    50521 gagataatca accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt
    50581 cgccgccaga cgtaaacaac gcgtcgcttc agtttcatcg ccctgcgcgt tggcgcatat
    50641 cgcctgccag aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt
    50701 ctccggataa tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct gtctggcatc
    50761 aaggtgctgc caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc
    50821 cccgccttcc cacgggtgga atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg
    50881 acccgtgaga tgccacaaat tgagcaactc tgctgtcatg tcgtcgcgtt tcagcgcaat
    50941 ttccagatta ttttccagac gcgccagtcg tttctccggt gtggctccgc ttaacttatc
    51001 aagcaaatcc cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc
    51061 aagataacgc gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc
    51121 gcgccagccg tcggcaaact ccggcgacat ctctacgcaa cgttgccata aggtaatggc
    51181 tttgttgtag ctacgcttgt tgtagtagaa gcaggccagt aaatgacggg caaaccagca
    51241 ctcttcaata ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg
    51301 cgggaagaca tcaatggctt ttgcgaccag ttcgccgcgt tcggctttcg gcaataaact
    51361 cgcctgcagg taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc
    51421 cagggtgggc attccccagt taatcagttg cccggcggtc atcagagcgt taacgtcgcg
    51481 tccctgacac agaccgcgcc actgcgcgag ggctgattcg ctacgaccat cgaaccagtt
    51541 cagccaccac agagtggcgt tcagcggata atcacgcagc agtttcgcgc gctgcaaacg
    51601 cgcgttgtcc tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg
    51661 attggttggg caggcacgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc
    51721 attacgcgcc gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca
    51781 gaccgcacgc cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact
    51841 ggcgcgaatc aaactggcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt
    51901 cagagcctga gtggcatacg ccaccgcttg cgggaaatcg gcgcggttat attccagcat
    51961 cgccagcgcc aggttacagc gataatccag cggatccagc accacgccgc gcaggtagta
    52021 atcgaacggt gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc
    52081 ttcatctgta ctggtaatgt cttgtgctgc gagtggtgct ttggcgacgt ccggcaacgg
    52141 caactcttgc ggctgatgtt cctgataact caggacaata ttgccgtcgg catcggagag
    52201 ctcaatggtc agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg
    52261 catcagtgcc acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag
    52321 gcggtatccg ttcaacggag agatggcata cagcccccac tcaatccccc gctcactacg
    52381 ctggagtttt atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg
    52441 caggaaatac tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg
    52501 gttatcggca aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact
    52561 cttatcccac gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc
    52621 aatatggtgg ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccaac
    52681 aaaatcgtac tgtgattttt cagccatata tgaggttgga acgggcacat ttttatagcg
    52741 agaaatgtcc actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa
    52801 agcggagacg gcccgtttgc cgtgatcaaa caccgccgtt acatccggcg ggaaaacgct
    52861 ctgatgccct tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt
    52921 ggcgttaccg ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt
    52981 gaaacctgtc atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg
    53041 tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg
    53101 atgttgcggc cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag
    53161 cgcaggttta atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc
    53221 gcgatgcacc cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag
    53281 ccacaccgcc tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta
    53341 gggataaacc gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat
    53401 atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg
    53461 agtcatggtg ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat
    53521 tgaacaatct ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg
    53581 aagatgagag cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg
    53641 gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt
    53701 tcgattccgg cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat
    53761 attgacgatg aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa
    53821 ggtgactgga cgctgtgcct gaacgtaacg cccaccagta ttgagtgtca ggttgctaat
    53881 gcttgtttaa gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag
    53941 gcgctgctgt ccgaaatcga aaaatgctgg catcgtcatc gcaaactgtg gccgggccgc
    54001 accatcaatc tggcgctggc aatccacggg caggttgatc cggtgaccgg cgtatcgcaa
    54061 accatgccgc aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaagagaag
    54121 ctgggcattc gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg
    54181 caaaataatt cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg
    54241 tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acagattggt
    54301 cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa
    54361 accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg
    54421 gttaatactc agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag
    54481 agtggagaac cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt
    54541 ctgtataact tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt
    54601 gcctttggtg agaactggct taatactatt attcgccaga caggatttaa cccgttcgac
    54661 cgcgacgaag gaccgagcgt gaaagcgacg caaattggct ttgggcaatt aagccgcgca
    54721 caacaggtgc tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg
    54781 cgcgataacg tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc
    54841 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    54901 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    54961 ttctgcttct ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt
    55021 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    55081 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcgaccagat caaccaggaa
    55141 cagatggtta tcagtaccgc cggaaaccac tttgtagccg cgttccagga acacttctac
    55201 catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    55261 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    55321 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
    55381 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    55441 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    55501 gaacaggtaa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc
    55561 ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc
    55621 cagatcggcg tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
    55681 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    55741 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    55801 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    55861 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
    55921 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    55981 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    56041 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    56101 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    56161 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    56221 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    56281 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    56341 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    56401 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    56461 atccctttac tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt
    56521 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    56581 cgtgaagccc tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg
    56641 ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa
    56701 cagtacaaca tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg
    56761 caggaagtgc tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat
    56821 cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc
    56881 gatttccgca ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag
    56941 ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg
    57001 aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat
    57061 ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg
    57121 cacaatcacg ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt
    57181 atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca
    57241 atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc
    57301 catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag
    57361 tcactgccgc gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct
    57421 aaaggtcagt ttgatagcga aggtctgatg gatttgagca aacaggaagg tgcgttcagc
    57481 gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa
    57541 cagttagtgg atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat
    57601 aaggtgctgt gatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa
    57661 attgccgcgt cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg
    57721 aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag
    57781 gtatcgacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc
    57841 atatactccg cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact
    57901 tcggtcaccg tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc
    57961 ttgaagggtt ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt
    58021 cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc
    58081 agctcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc
    58141 gcgtgggtga cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac
    58201 tgattacgtg cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct
    58261 actaatgcat cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg
    58321 accaactggc gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg
    58381 gagaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc
    58441 agaagcggaa tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg
    58501 aggcggtaat agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg
    58561 tgagtggcag aaataatccg cacgtcgata tcaatatcgc ggttgctgcc cagcgggcgc
    58621 actttacgct cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca
    58681 atttcatcga gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg
    58741 acagcgccag taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg
    58801 ggcaatgcgc cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg
    58861 atagcctggg cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg
    58921 tctgattgcg ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg
    58981 acaattgcct cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca
    59041 attgcctgat atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc
    59101 tgtgttgcag caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt
    59161 cccggctgca ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc
    59221 aggtcgctga taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca
    59281 ctttccgccg tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc
    59341 aatcccggat cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac
    59401 ctcatgggtg gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc
    59461 agccggtttg cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt
    59521 ggtgagttcc agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct
    59581 gtcgctggac tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag
    59641 cgcctgaccg tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact
    59701 taacgcttca atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc
    59761 gagcaaaatc ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg
    59821 ctgacgcgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag
    59881 cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag
    59941 atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt
    60001 attgtgattc tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg
    60061 tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag
    60121 tgggcgtaat tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat
    60181 atcgtacgtg aagatttcga gcactgcccg gggccgaact gacaaataaa atagcctgat
    60241 aggataggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa
    60301 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    60361 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc
    60421 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaattggcgt gccggtattg
    60481 atgacatcaa tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat
    60541 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    60601 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    60661 gcgggcaggc tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc
    60721 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    60781 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    60841 ggcccgacaa cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    60901 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    60961 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    61021 gaaacgctat tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt
    61081 ttcaccggcc cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat
    61141 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    61201 tgcccacgag agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca
    61261 aaggcttcaa gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg
    61321 gccagattgt tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    61381 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    61441 gtaggatcgt ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    61501 gtcatcgctt cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga
    61561 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga
    61621 attagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac aggccagcgt
    61681 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    61741 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    61801 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    61861 tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    61921 agccactgct tactatatgc agtaactgtg ccaataaaga aaatagtttg gtaacgtact
    61981 gattatacgt cgctttgagg ggtttatggc ttctccgtta tttgaatgta aatcagccac
    62041 tgtgtcgcta aaaagagaca acttaagata aacttattag ataatattaa aatcaatgag
    62101 ttaagtgtcg ccagaaagcg acacggtaaa ccacccattg tcgtgattta cagacacaaa
    62161 aaagctcccg gagttgggag cttatgatag tggttggtgc ttaatgccgg atgcggcgta
    62221 aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact gcttacgcgc
    62281 attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat gccaggagtt
    62341 gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc gaccactttc
    62401 agtcgtaatc gacgtaatac cgttcggtga accgttcggg ttagccgggt aggtttcagt
    62461 gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt ccagtgccgc
    62521 cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag caatcggcat
    62581 ttgcgagccc accatcccct gcaacagcag cgacgggctt tgggtcactt caaccaggct
    62641 gaaacgcgct tcaaagcgat cggaggtatt gcgcacaaaa cgtggccaca actcactacc
    62701 tgggatcagt tcgcgcaggt tagacatcat ctggcaaccg ttacataccc ccagcgccag
    62761 cgtttgcgga cggtggaaga aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat
    62821 tgacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac caccgcacgc
    62881 gaccagggcg tggaaatctt ccaggcccgt gcgtccagtc agcaggtcac tcatatgcac
    62941 atcgattgca tcgaagcctg cacggtggaa agctgccgcc atttcaacat gcgagttcac
    63001 gccctgctca cgcagcacag caactttcgg acgtgcgcca gtggcaatat acggtgctgc
    63061 cacatcttcg ttgatatcga acgacagttt cacattcagg cccggatcgg cgtcgtttga
    63121 tttcgcctgg tgctcctggt cagcacactc cgggttgtca cgcaggcgct gcatctgcca
    63181 ggtagtttct gcccaccaga cacgcaacgt ggtgcggctt tcgctgaata cagtctgccc
    63241 gttggcggta atcacaaaac ggtcaccgga aaccgcctgc cctacataat ggacacaatc
    63301 agcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg cacgaacctg
    63361 aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcgc ccagagaggc
    63421 aatatccgcg ttaatgccgc aatgaccagc aaaggccatt tccgccagcg ttaccagcag
    63481 gccgccatca gagcggtcgt ggtatgcgag cagcttacgc tgtgcaacca gcgcctgaat
    63541 cgcgtcatag aagcctttca gttgcgcgac atcgcgtaca tctgccggtt tgtcgccaag
    63601 ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc
    63661 aatcagcagc agtgcgttat cttcggtaga aagctgcggc gtgatggtgt gacgtacatc
    63721 ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt cgcgctcttc
    63781 gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg ggatcgtcag
    63841 gcccagcgcc ggacaaagct cttcgcccac ggctttaacg gcttcataca ggcccgcatc
    63901 ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc gtttgatatc
    63961 gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac gggcagaggc
    64021 ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt caccgtagta
    64081 gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc acggacccac
    64141 catctgatcg cgcgctacca tgccagttac gctgcggtca ccgatggtca ccaggaaagt
    64201 tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc
    64261 ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg
    64321 cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa aatgacgatc
    64381 gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg gtgcgcgctc
    64441 acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca gcacgtagcg
    64501 ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt cgcttagaat
    64561 atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg gcatcgcgtt
    64621 agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat caccaagctg
    64681 ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt cgcgctgtac
    64741 ggaagcaaag tcgaggtcgg catcagactg acctgacgcc atggaagacg ccgctccgcc
    64801 gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac cgacgttgat
    64861 ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca gcatgatcgg
    64921 tttgtgataa ccgcgcagct cttcgccgtt gtggctgttc actttttctt cataagtacg
    64981 gaagtagcca ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcgc cgcccagcgg
    65041 gccttcggtc atgatgtcca gcgcggtgac aatgcgctca ggcttaccga aatcttcttc
    65101 ccacggctgt tcgaagccag gaattcgcag gttggaaacg gagaaaccaa ccaaacccgc
    65161 tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt
    65221 cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga ctttcatcag
    65281 aatatgcgcc ggttcctgat ggaaatcgta gcggcccgtt tcgtggtcag caaagtagcg
    65341 gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga gaacgtaatc
    65401 tggcgtggtt tcgaaggtgt ttttgatcat cttaaacagc gatttcggct gttgctcacc
    65461 gtcgataacc caatcggcgt taaaaatctt gtggcggcag tgctcggagt tcgcctgggc
    65521 gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag catcctgcag
    65581 atagtcaatt tcatcttccg ccagtgccag gccaagacgc aggttagcgt cgattagcgc
    65641 ctgacggccc tgccccagca aatcaacgct ggtgaccgga gtcggttgat ggtgtgcaaa
    65701 cagctgctct gcatcatcta aagcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc
    65761 agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat agtaagcaac
    65821 gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat cggtcgcttt
    65881 cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt tgccttgcgg
    65941 ggcgtggctg gcgagtgccg ggccatattt cagcaggcgt tcaagttgtg cgtgctcatc
    66001 atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac
    66061 cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg ccgacagtgc
    66121 aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt cggtgtaccc
    66181 aaggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg
    66241 gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc
    66301 atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc actattatta
    66361 cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa aaattaaaga
    66421 ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc tggccatcca
    66481 ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc
    66541 gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa ccttttggcc
    66601 tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg aaagtgaccg
    66661 tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc gacctgctgg
    66721 cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc cctacctatt
    66781 attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt acgctgggca
    66841 acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtggttaac gatctccaga
    66901 ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa aaaggctctg
    66961 cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct gattctgtcg
    67021 ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat atcaccgatg
    67081 aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc gccgccctgc
    67141 tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa gagaaatacc
    67201 tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc gccgtcgatg
    67261 cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt gactggcgtt
    67321 tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact tcaccgacgg
    67381 gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc attacggatc
    67441 gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat atgatgagta
    67501 aagtgccgga aagtgtgccg gagaacgagc ggatctggtt tgccctcgct gcgtacaata
    67561 tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg
    67621 acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc tattacagca
    67681 agctgactta cggctacgct cgtggacatg aagcctacgc ttatgtcgaa aatattcgta
    67741 agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct acagaagcgg
    67801 cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc aaagagaaat
    67861 ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat tctccctctc
    67921 tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag gattgcgctt
    67981 ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc aacgctgcgc
    68041 actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc ggatgatgca
    68101 gcacatccat taaagatccc gcagcgccag ttttcgcgtc atgcgcacca aagaccacgc
    68161 gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca agcgtgacat
    68221 acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc cgcagggcca
    68281 tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag ccttcgccga
    68341 ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca tcccaggcac
    68401 gtttcgccag cgtcagcgcg tgacgcatcc agtattcgtg gctaaattcg acttcagaca
    68461 aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat gctttactct
    68521 attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga cagaaataaa
    68581 gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc ggagtgccga
    68641 ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc aatacccaac
    68701 caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc cagggcgtat
    68761 cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag atatcagcat
    68821 cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg gtaacattgt
    68881 cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg ctgtgaccaa
    68941 aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct tttatcaata
    69001 acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc ggttggcgac
    69061 gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt
    69121 caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc
    69181 ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc cggcaacaat
    69241 cgaccgcatc atggctaact ggaattttta attcatggca attagcggca atggaatata
    69301 aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc gcgaggaaag
    69361 atatgaacgg cttacttcgt atccgtcagc gttaccaggg gcttgcccag agtgataaaa
    69421 aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagt tctcagcaac
    69481 tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct
    69541 ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc cagccggaat
    69601 cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg
    69661 aaaaactgat taaagaaaat actgccgcta tgtacgcaac gctaaacgtt aatagtgaag
    69721 agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt ctgaccggta
    69781 ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag attggcttca
    69841 atgctgccgc agtgcgcgat atgcatgcgc tactcgcaac agtacaggcg tcgtcccctg
    69901 acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac ctggcggcag
    69961 atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact cccaatgccc
    70021 tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg acaaacagtg
    70081 cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc attgcgctga
    70141 ttcagcagga tctggaactg gcaccagaac gtattcgtca gagtgaagcg ctgatgaaaa
    70201 aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt
    70261 ttccttatgg cgttgttaat cactaaaaaa tgcatcaatt gtgatatgtg tg
//
LOCUS       DAZGCS010000011        67359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGCS010000011 DAZGCS010000000
VERSION     DAZGCS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.011"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..2737)
                     /gene="mgtA"
                     /locus_tag="ACHK01_000755"
     CDS             complement(41..2737)
                     /gene="mgtA"
                     /locus_tag="ACHK01_000755"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO8095751.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2878..2931)
                     /gene="mgtL"
                     /locus_tag="ACHK01_000756"
     CDS             complement(2878..2931)
                     /gene="mgtL"
                     /locus_tag="ACHK01_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO8095752.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3116..4063
                     /gene="treR"
                     /locus_tag="ACHK01_000757"
     CDS             3116..4063
                     /gene="treR"
                     /locus_tag="ACHK01_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO8095753.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4182..5603
                     /gene="treB"
                     /locus_tag="ACHK01_000758"
     CDS             4182..5603
                     /gene="treB"
                     /locus_tag="ACHK01_000758"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO8095754.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5653..7308
                     /gene="treC"
                     /locus_tag="ACHK01_000759"
     CDS             5653..7308
                     /gene="treC"
                     /locus_tag="ACHK01_000759"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO8095755.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKLEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            7702..9840
                     /gene="nrdD"
                     /locus_tag="ACHK01_000760"
     CDS             7702..9840
                     /gene="nrdD"
                     /locus_tag="ACHK01_000760"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO8095756.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            9998..10462
                     /gene="nrdG"
                     /locus_tag="ACHK01_000761"
     CDS             9998..10462
                     /gene="nrdG"
                     /locus_tag="ACHK01_000761"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO8095757.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QLFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10507..10893)
                     /gene="cybC"
                     /locus_tag="ACHK01_000762"
     CDS             complement(10507..10893)
                     /gene="cybC"
                     /locus_tag="ACHK01_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO8095758.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11076..12428)
                     /gene="pmbA"
                     /locus_tag="ACHK01_000763"
     CDS             complement(11076..12428)
                     /gene="pmbA"
                     /locus_tag="ACHK01_000763"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO8095759.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            12522..13073
                     /gene="yjgA"
                     /locus_tag="ACHK01_000764"
     CDS             12522..13073
                     /gene="yjgA"
                     /locus_tag="ACHK01_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO8095760.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(13229..14602)
                     /gene="mpl"
                     /locus_tag="ACHK01_000765"
     CDS             complement(13229..14602)
                     /gene="mpl"
                     /locus_tag="ACHK01_000765"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO8095761.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            14778..15776
                     /gene="fbp"
                     /locus_tag="ACHK01_000766"
     CDS             14778..15776
                     /gene="fbp"
                     /locus_tag="ACHK01_000766"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO8095762.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(15809..16804)
                     /gene="yjfF"
                     /locus_tag="ACHK01_000767"
     CDS             complement(15809..16804)
                     /gene="yjfF"
                     /locus_tag="ACHK01_000767"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO8095763.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16791..17813)
                     /gene="ytfT"
                     /locus_tag="ACHK01_000768"
     CDS             complement(16791..17813)
                     /gene="ytfT"
                     /locus_tag="ACHK01_000768"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO8095764.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(17827..19329)
                     /gene="ytfR"
                     /locus_tag="ACHK01_000769"
     CDS             complement(17827..19329)
                     /gene="ytfR"
                     /locus_tag="ACHK01_000769"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO8095765.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(19469..20425)
                     /gene="ytfQ"
                     /locus_tag="ACHK01_000770"
     CDS             complement(19469..20425)
                     /gene="ytfQ"
                     /locus_tag="ACHK01_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO8095766.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            20735..21265
                     /gene="ppa"
                     /locus_tag="ACHK01_000771"
     CDS             20735..21265
                     /gene="ppa"
                     /locus_tag="ACHK01_000771"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO8095767.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(21345..21695)
                     /gene="chpB"
                     /locus_tag="ACHK01_000772"
     CDS             complement(21345..21695)
                     /gene="chpB"
                     /locus_tag="ACHK01_000772"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO8095768.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(21689..21940)
                     /gene="chpS"
                     /locus_tag="ACHK01_000773"
     CDS             complement(21689..21940)
                     /gene="chpS"
                     /locus_tag="ACHK01_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO8095769.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(22153..22494)
                     /gene="ytfP"
                     /locus_tag="ACHK01_000774"
     CDS             complement(22153..22494)
                     /gene="ytfP"
                     /locus_tag="ACHK01_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO8095770.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(22497..26276)
                     /gene="tamB"
                     /locus_tag="ACHK01_000775"
     CDS             complement(22497..26276)
                     /gene="tamB"
                     /locus_tag="ACHK01_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO8095771.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAAERWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNCTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(26273..28006)
                     /gene="tamA"
                     /locus_tag="ACHK01_000776"
     CDS             complement(26273..28006)
                     /gene="tamA"
                     /locus_tag="ACHK01_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO8095772.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            28212..28850
                     /gene="msrA"
                     /locus_tag="ACHK01_000777"
     CDS             28212..28850
                     /gene="msrA"
                     /locus_tag="ACHK01_000777"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO8095773.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            29173..30516
                     /gene="paeA"
                     /locus_tag="ACHK01_000778"
     CDS             29173..30516
                     /gene="paeA"
                     /locus_tag="ACHK01_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO8095774.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(30578..30784)
                     /gene="ytfK"
                     /locus_tag="ACHK01_000779"
     CDS             complement(30578..30784)
                     /gene="ytfK"
                     /locus_tag="ACHK01_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO8095775.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            31109..31666
                     /gene="ytfJ"
                     /locus_tag="ACHK01_000780"
     CDS             31109..31666
                     /gene="ytfJ"
                     /locus_tag="ACHK01_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8095776.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(31656..32396)
                     /gene="cysQ"
                     /locus_tag="ACHK01_000781"
     CDS             complement(31656..32396)
                     /gene="cysQ"
                     /locus_tag="ACHK01_000781"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO8095777.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            32586..34529
                     /gene="cpdB"
                     /locus_tag="ACHK01_000782"
     CDS             32586..34529
                     /gene="cpdB"
                     /locus_tag="ACHK01_000782"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO8095778.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(34658..35038)
                     /gene="ytfH"
                     /locus_tag="ACHK01_000783"
     CDS             complement(34658..35038)
                     /gene="ytfH"
                     /locus_tag="ACHK01_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO8095779.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            35127..35987
                     /gene="qorB"
                     /locus_tag="ACHK01_000784"
     CDS             35127..35987
                     /gene="qorB"
                     /locus_tag="ACHK01_000784"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8095780.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            36096..37061
                     /gene="ytfF"
                     /locus_tag="ACHK01_000785"
     CDS             36096..37061
                     /gene="ytfF"
                     /locus_tag="ACHK01_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8095781.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            37169..37831
                     /gene="ytfE"
                     /locus_tag="ACHK01_000786"
     CDS             37169..37831
                     /gene="ytfE"
                     /locus_tag="ACHK01_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO8095782.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(37876..39288)
                     /gene="cycA"
                     /locus_tag="ACHK01_000787"
                     /gene_synonym="dagA"
     CDS             complement(37876..39288)
                     /gene="cycA"
                     /locus_tag="ACHK01_000787"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO8095783.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(39597..40217)
                     /gene="fklB"
                     /locus_tag="ACHK01_000788"
     CDS             complement(39597..40217)
                     /gene="fklB"
                     /locus_tag="ACHK01_000788"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8095784.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            40436..41074
                     /gene="ytfB"
                     /locus_tag="ACHK01_000789"
     CDS             40436..41074
                     /gene="ytfB"
                     /locus_tag="ACHK01_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO8095785.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(41058..41705)
                     /locus_tag="ACHK01_000790"
     CDS             complement(41058..41705)
                     /locus_tag="ACHK01_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8095786.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            41918..42709
                     /locus_tag="ACHK01_000791"
     CDS             41918..42709
                     /locus_tag="ACHK01_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO8095787.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            42719..43561
                     /locus_tag="ACHK01_000792"
     CDS             42719..43561
                     /locus_tag="ACHK01_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8095788.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            43571..44347
                     /locus_tag="ACHK01_000793"
     CDS             43571..44347
                     /locus_tag="ACHK01_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO8095789.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            44357..45898
                     /locus_tag="ACHK01_000794"
     CDS             44357..45898
                     /locus_tag="ACHK01_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8095790.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            45895..47976
                     /locus_tag="ACHK01_000795"
     CDS             45895..47976
                     /locus_tag="ACHK01_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO8095791.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            47997..48473
                     /locus_tag="ACHK01_000796"
     CDS             47997..48473
                     /locus_tag="ACHK01_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO8095792.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            48516..49817
                     /locus_tag="ACHK01_000797"
     CDS             48516..49817
                     /locus_tag="ACHK01_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO8095793.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            49870..50841
                     /locus_tag="ACHK01_000798"
     CDS             49870..50841
                     /locus_tag="ACHK01_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO8095794.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENFYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(50953..51402)
                     /gene="rplI"
                     /locus_tag="ACHK01_000799"
     CDS             complement(50953..51402)
                     /gene="rplI"
                     /locus_tag="ACHK01_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO8095795.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(51444..51671)
                     /gene="rpsR"
                     /locus_tag="ACHK01_000800"
     CDS             complement(51444..51671)
                     /gene="rpsR"
                     /locus_tag="ACHK01_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO8095796.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(51676..51990)
                     /gene="priB"
                     /locus_tag="ACHK01_000801"
     CDS             complement(51676..51990)
                     /gene="priB"
                     /locus_tag="ACHK01_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO8095797.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(51997..52392)
                     /gene="rpsF"
                     /locus_tag="ACHK01_000802"
     CDS             complement(51997..52392)
                     /gene="rpsF"
                     /locus_tag="ACHK01_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO8095798.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            52719..52994
                     /gene="yjfY"
                     /locus_tag="ACHK01_000803"
     CDS             52719..52994
                     /gene="yjfY"
                     /locus_tag="ACHK01_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO8095799.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(53123..53809)
                     /gene="ulaF"
                     /locus_tag="ACHK01_000804"
     CDS             complement(53123..53809)
                     /gene="ulaF"
                     /locus_tag="ACHK01_000804"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO8095800.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(53809..54663)
                     /gene="ulaE"
                     /locus_tag="ACHK01_000805"
     CDS             complement(53809..54663)
                     /gene="ulaE"
                     /locus_tag="ACHK01_000805"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO8095801.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(54673..55323)
                     /gene="ulaD"
                     /locus_tag="ACHK01_000806"
     CDS             complement(54673..55323)
                     /gene="ulaD"
                     /locus_tag="ACHK01_000806"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8095802.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(55337..55801)
                     /gene="ulaC"
                     /locus_tag="ACHK01_000807"
     CDS             complement(55337..55801)
                     /gene="ulaC"
                     /locus_tag="ACHK01_000807"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO8095803.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(55811..56116)
                     /gene="ulaB"
                     /locus_tag="ACHK01_000808"
     CDS             complement(55811..56116)
                     /gene="ulaB"
                     /locus_tag="ACHK01_000808"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO8095804.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSKIDHTVNSCAVGEY
                     KSELSNADIIIASTHIAGEISVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(56132..57529)
                     /gene="ulaA"
                     /locus_tag="ACHK01_000809"
     CDS             complement(56132..57529)
                     /gene="ulaA"
                     /locus_tag="ACHK01_000809"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8095805.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYN
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            57884..58948
                     /gene="ulaG"
                     /locus_tag="ACHK01_000810"
     CDS             57884..58948
                     /gene="ulaG"
                     /locus_tag="ACHK01_000810"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO8095806.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVASGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            59056..59811
                     /gene="ulaR"
                     /locus_tag="ACHK01_000811"
     CDS             59056..59811
                     /gene="ulaR"
                     /locus_tag="ACHK01_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO8095807.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(59808..60557)
                     /gene="yjfP"
                     /locus_tag="ACHK01_000812"
     CDS             complement(59808..60557)
                     /gene="yjfP"
                     /locus_tag="ACHK01_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8095808.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            60739..61068
                     /gene="bsmA"
                     /locus_tag="ACHK01_000813"
     CDS             60739..61068
                     /gene="bsmA"
                     /locus_tag="ACHK01_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO8095809.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            61217..61492
                     /gene="yjfN"
                     /locus_tag="ACHK01_000814"
     CDS             61217..61492
                     /gene="yjfN"
                     /locus_tag="ACHK01_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO8095810.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(61609..63234)
                     /gene="aidB"
                     /locus_tag="ACHK01_000815"
     CDS             complement(61609..63234)
                     /gene="aidB"
                     /locus_tag="ACHK01_000815"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO8095811.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(63318..64481)
                     /gene="yjfC"
                     /locus_tag="ACHK01_000816"
     CDS             complement(63318..64481)
                     /gene="yjfC"
                     /locus_tag="ACHK01_000816"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8095812.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(64484..65122)
                     /gene="yjfM"
                     /locus_tag="ACHK01_000817"
     CDS             complement(64484..65122)
                     /gene="yjfM"
                     /locus_tag="ACHK01_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO8095813.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(65132..65530)
                     /gene="yjfL"
                     /locus_tag="ACHK01_000818"
     CDS             complement(65132..65530)
                     /gene="yjfL"
                     /locus_tag="ACHK01_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO8095814.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(65548..66207)
                     /gene="yjfK"
                     /locus_tag="ACHK01_000819"
     CDS             complement(65548..66207)
                     /gene="yjfK"
                     /locus_tag="ACHK01_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO8095815.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(66258..66956)
                     /gene="yjfJ"
                     /locus_tag="ACHK01_000820"
     CDS             complement(66258..66956)
                     /gene="yjfJ"
                     /locus_tag="ACHK01_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO8095816.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(66975..>67359)
                     /gene="yjfI"
                     /locus_tag="ACHK01_000821"
     CDS             complement(66975..>67359)
                     /gene="yjfI"
                     /locus_tag="ACHK01_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO8095817.1"
                     /translation="ALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSQVDNALDLAEITEEYSH"
BASE COUNT        16501 a        17866 c        16865 g        16127 t
ORIGIN      
        1 ctgacctgaa agagtatttc aggccagcct catcacttgc ttactgccag ccgtaacgac
       61 ggctataaaa tcctttcacc aactgggtta acgtcatata ccctgccaaa atcgcaacca
      121 gccacgggaa atagcttaac ggcagcgcct gcagctgcag ataactggcc agcggtgaaa
      181 acggcaatgc gatcccgaca atcatcacga tcacggtcat gatcattaac ggccacgatg
      241 cacagctctg aataaacggc acacggcggg tgcggatcat atgcacaatc agcgtttgcg
      301 acagtaagcc caccacaaac catcccgact ggaacagcgt ttgcgtttcc ggcgtgttgg
      361 catggaatac ccaccacatc aggcaaaacg tcagaatatc gaagatcgag ctgatcggtc
      421 cgaagaagat cataaagcgc cccagatccg ccggattcca acgctgcggc ttttgaattt
      481 gctcgtcgtc gacgttatca aacgggatcg ccacctgtga cacatcatac agcaggttct
      541 gaataagcaa gtgtaacggc aacatcggca ggaagggcaa gaaagcactc gccaccagca
      601 cgctgaacac attaccgaag ttggagctcg ccgtcatttt gatgtatttc agcatgttgg
      661 cgaaagtgcg acgtccttca ataaccccct cttccagcac catcaggctt ttttccagca
      721 ggatgatatc agccgcttca cgggcaatat ctaccgcgcc gtccacagaa atgccgatat
      781 ccgcagcgcg taaggcgggc gcatcattaa taccatcgcc cataaagcca accacatgcc
      841 cttcgcgctt cagtaaggtc acaatgcgtt ctttatgcat cggcgtcagg cgggcaaaca
      901 gcgtggtgcg ctgtgcgaga tttgccagtt cgtcgtcaga tagcgtttca atatcactac
      961 caatgaccac ctctcccgcg tccagcccca cttcatggca cactttcgct gcaactaact
     1021 cactgtcgcc agtaaggatt tttacggtga ttccactcgc ttttaatgcc ttcagtgccg
     1081 gagcggttgt ctcttttggc ggatcaagga aagcaatata tccttcgagg atcaggtcgg
     1141 attcatccgc ccgctggtaa tctccttcac gcgctggcag gtatttcgtc gccaccgcaa
     1201 ccacgcgcag cccctgacga ttaagcgtat cagtaacccg cttaatctta cgcagcatga
     1261 tgtcatcgag cggcacaatc tcgccattgt gacgcacctg cgaacacaca ttgaggattt
     1321 cctgcaatgc acctttgcaa accagctgat ggtgctcggt attttctgcc actaccaccg
     1381 acatccggcg acgctcgaaa tcaaacggaa tctcatcaat tttttgccaa cgactggcca
     1441 gcgagcgcgc tgactcttca tccgtacctt cgagcaccgc tgtatcgagc aggtttttaa
     1501 gtccggtctg ataatgactg ttcaaccacg cgctatgcag cacgcgttcg ctggttttac
     1561 cggagatatc ggtatgattc tccagcacaa ttttgtcctg tgtcagggtg ccagttttat
     1621 cagtgcacag aatatccatt gcgccaaagt tctgaatagc atccagatgt ttgacgatca
     1681 ctttctgttt cgacagcttt actgcgccgc gcgccagcgt cgaggtaaca atcatcggca
     1741 acatttccgg cgttaagcct accgcgaccg aaagcgcaaa cagtgccgct tcccaccagt
     1801 cgcctttggt gtaaccattg attaacagca ccaccggagc catcaccagc ataaagcgaa
     1861 tcagcagcat actgacgcgg ctgatccctt gctgaaaggc attcggctcg ctttcctgct
     1921 cactaacacg ccccgccagt tgaccaaacc aggtattggc acctgtagca atcaccattg
     1981 cttgtgcagt gccgctcacc acggtggtgc ccataaaaca cagggtgtca cactccagcg
     2041 gattgctgtg ctccggctgg cgagtggtag cggctttttc tacgggcaga gactcaccgg
     2101 ttaacgacgc ctgagcgacg aacagatccc gcgcctgcaa gatacgtaaa tctgccggga
     2161 tcatatcccc cgccgccagt tttattatat cgccgggcac cagctggtcg atcgggatct
     2221 ccagccagcc attttcgcct ttgtcgttaa ttacgcgcag aaccgtcgct gtattgctga
     2281 ccatcgcttt cagggcatct gccgctttag tggaacgtgc ttcctgaata aagttcagca
     2341 acgtagaaat agcgaccatt agcgcgataa cgcccgcggc aaataaatct tccgtggcgt
     2401 aagaaatagc gccgagaatg gtgagtaaaa tattaaaggg gttgcgatag cagacccata
     2461 aatgtaccca ccacggcgac ggttgttgtg cgggtaattt attttcacca tgttgttcgc
     2521 gggcagattc cacttccgcc tgatttaacc cttccggatg ggtgtcgaac gttttccata
     2581 attcttcttc gggcatcacc gccattttca ggcaatgcgc acttaaggac ggcgggaccg
     2641 ccgtattcac tgtctgctgc gcgccaggca atggatcgcg atgaaccaga cgggaaggta
     2701 aatggcgaat gagccgggta aaaatttctt taaacataag gagtccctcc gcactgtctg
     2761 aaacagtaca gaaatgtctt caggcgtggc gaagccttac ctgaaggtaa taaagctgag
     2821 cagggacgtt aaaacgtcac ggccttacgc atccggtaac gccgtgaaaa gacgggctta
     2881 ccggaaaagt tttaatctcc gtcgagggag aggcgtggga tcaggttcca tataacctcc
     2941 ggtaagtgaa aaaagcgccg caaataataa ggagaatcca ataatattcg tagcatctat
     3001 ggcgatataa tacctgctgg caactaaacc aacgataaac cagactttac ccattgctga
     3061 atgcacgggt aaagttaggc tcaaataatt aaacaacacg ttacaggaca acaggatgca
     3121 aaatcggctg accatcaaag acatcgcacg cttaagcggc gtggggaaat ctacagtttc
     3181 ccgggtgctg aataacgaaa gcggcgtgag ccagcgcacc cgcgagcgtg ttgaagcagt
     3241 gatgaatcag catggatttt ccccttcccg ctctgcgcgc gctatgcgtg ggcaaagcga
     3301 taaagtggtc gccatcattg ttacccgcct ggattcgttg tcagaaaatc tcgccgttca
     3361 aaccatgctg ccagcgttct atgaacaagg ttacgaccca atcatgatgg aaagtcagtt
     3421 ttccccgcaa ttagttgccg aacatttggg ggtgctgaaa cggcgtaata tcgacggcgt
     3481 agtgctgttc ggttttactg gcataacaga agaaatgtta gcccactggc agtcatcgct
     3541 ggttctgctg gcgcgtgacg caaaaggttt tgcttcggtc tgttatgacg acgaaggggc
     3601 aatcaagatc ctgatgcaac ggctgtatga ccaggggcat cgtaatatca gttatctcgg
     3661 cgtgccgcat agtgacgtga cgaccgggaa gcgacgtcac gaagcctacc tggcgttctg
     3721 caaagcgcat aaactgaatc ccgttgccgc tctgccaggc cttgctatga agcaaggcta
     3781 tgagaacgta gcaaaagtga ttacgcctga aactaccgcc ttactgtgcg caaccgacac
     3841 gctggcactt ggcgcaagta aatacctgca agagcaacgc atcgacacct tgcaactggc
     3901 gagcgtcggt aatacgccat taatgaaatt ccttcatccg gagatcgtca ccgtagatcc
     3961 tggttacgcc gaagctggac gccaggcggc ctgccaattg atcgcacagg taaccgggcg
     4021 cagcgaaccg caacaaatca tcatccccgc caccctgtcc tgatcgtttc ctgaacgata
     4081 aattgtgatc ttcgctgcgt ttcgggaacg ttcccgtttt taaatttttc cgcgcaatat
     4141 attctgcagc caaccaaaaa tgtcatctgc catggggctt tatgatgagc aaaataaacc
     4201 aaacggatat cgatcggttg attgaactgg tcggcgggcg cggcaatatt gcgacggtga
     4261 gccactgtat tactcgcctg cgctttgtcc tcaaccaacc ggccaatgcc agaccgaaag
     4321 aaattgagca actccccatg gtgaaaggct gtttcaccaa tgccgggcaa tttcaggtgg
     4381 tgattggcac caacgtgggt gattactatc aagcactaat agcgtcaacc ggacaggcgc
     4441 aggttgataa agagcaggta aaaaaagccg cccggcagaa tatgaaatgg catgagcagt
     4501 tgatctctca tttcgcggag atcttcttcc cgttgctgcc cgcgttgatt agcggcggtt
     4561 tgatcctcgg ttttcgtaat gtgatcggcg atttgcccat gagcaacggt caaacgctgg
     4621 cgcaaatgta cccttccctg caaacgatct acgattttct gtggttgatc ggtgaagcga
     4681 tcttcttcta cctgccggtc gggatttgct ggtcagcggt gaaaaaaatg ggcggcacgc
     4741 cgatccttgg tatcgtgctt ggcgtgacac tggtttcccc ccagctgatg aacgcttatc
     4801 tgctcgggca gcagctgccg gaagtgtggg actttggcat gttcagcatc gccaaagtgg
     4861 gctatcaggc gcaggtgatc ccggcactgt tagccgggct ggcgctgggc gttattgaaa
     4921 ctcgccttaa acgcatcgta ccggattacc tctatctggt ggtggtgccc gtctgttcgc
     4981 tgatcctcgc ggtgttcctc gcccatgcgc tgattggtcc gtttggtcgc atgattggcg
     5041 atggcgttgc ctttgcggta cgtcacctga tgaccggcag ctttgctccg attggtgcgg
     5101 cattgtttgg cttcctgtac gcgccgctgg tgatcaccgg cgtacaccag accacccttg
     5161 ctattgattt gcagatgatt caaagcatgg gtggcacgcc agtgtggccg ctgattgcgc
     5221 tgtcgaatat cgctcagggc tccgccgtga taggcattat catttccagc cgcaagcaca
     5281 atgaacgcga gatctccgtg cctgccgcta tctccgcctg gcttggggtc actgagcctg
     5341 caatgtacgg catcaacctg aaatatcgct tcccgatgct gtgcgcgatg attggttctg
     5401 gtctggcagg attactatgc ggcctgaacg gcgttatggc gaatggtatc ggcgtaggcg
     5461 gcctgccggg aattctctcg attcaaccga gctactggca ggtatttgcg ctggcaatgg
     5521 ttatcgccat catcatcccg attgtactca cctcgtttat ctatcagcgg aaataccgcc
     5581 tgggcacgct ggatattgtt taattttctt cggggcgcaa ttgcgccccc tcgcatttgc
     5641 aggaataacg taatgactaa tcttccccac tggtggcaaa acggcgttat ctaccagatt
     5701 tatccaaaga gttttcagga caccacgggt agcggcaccg gcgatttacg tggcgttatc
     5761 caacgcctgg actatctgca taaactgggc gttgatgcca tctggctaac ccccttttat
     5821 atctctcccc aggtcgataa cggttacgac gtagcgaact atacggcgat tgatcccacc
     5881 tacggcacgc tggacgattt tgacgaactg gtgacgcagg caaaatcgcg cgggattcgt
     5941 atcattctcg atatggtgtt taaccatacc tctacccaac atgcctggtt tcgcgaggcg
     6001 ctgaacaaag aaagccctta ccgccagttt tatatctggc gcgatggcga accagaaacg
     6061 ccaccgaaca actggcgttc aaaatttggc ggtagtgcgt ggcgctggca tgcggaaagc
     6121 gaacagtact atttgcatct ctttgcacca gaacaggcgg atctcaactg ggagaatcca
     6181 gcggtacgcg cagagctgaa aaaagtctgt gaattctggg ccgatcgtgg ggtcgacggg
     6241 ttgcgcctgg atgtggtgaa tctgatctcc aaagacccgc gtttccctga agacctggat
     6301 ggcgacgggc gtcgctttta caccgacggg ccacgagcac acgagttttt gcacgagatg
     6361 aaccgcgatg tgtttacgcc acgcgggtta atgaccgtag gtgaaatgtc ctccaccagc
     6421 cttgagcatt gccagcgata cgcggcactg acaggcagtg aattgtcgat gacctttaat
     6481 tttcatcacc tgaaggtcga ttatcccggt ggtgaaaaat ggacgctggc taaacctgac
     6541 tttgtggcgt tgaaaacatt gttccgccac tggcaacaag gaatgcacaa cgtggcatgg
     6601 aatgccttgt tctggtgtaa ccacgatcag ccgcgcatta tttctcgctt tggtgatgaa
     6661 ggtgaatacc gcgtgcctgc ggcaaaaatg ctggcgatgg tgctgcatgg catgcaggga
     6721 acgccgtata tctaccaagg cgaagagatt ggcatgacca acccgcattt cacgcgcatt
     6781 actgactatc gcgacgtgga gagcctcaat atgtttgccg agctgcgcaa cgatggtcgt
     6841 gatgccgacg agttattggc aatcctcgcc agtaaatccc gtgacaacag ccgcacgccc
     6901 atgcaatgga gcaacggcga taatgccggg tttacggctg gcgaaccgtg gattggcctg
     6961 ggcgataact atcaacaaat caacgtagaa gccgcgctgg ccgatgactc ctcggtgttt
     7021 tacacctacc aaaagttaat cgcactgcgt aagctagaag ccatcctgac atggggcaat
     7081 taccaggatc tgctgccaaa cagccctgta ttgtggtgct atcgccgtga atggaagggg
     7141 caaaccttgc tggtcattgc caaccttagc cgtgagatcc aaccctggca gccagggcaa
     7201 atgcgcggca actggcagct tgtgatgcat aactacgaag aagcctcacc acaaccctgt
     7261 gccatgactt tacggccttt tgaggctgtc tggtggttac agaagtaagt atccgctaag
     7321 ccccgggaat gcctgtcatt ccggggtttt gtctgtcagc atgttgtttt tgttgatttt
     7381 tcaaccagca aattcattaa aaaatttaca taacgctgta gcgcccgtca tccgtacgct
     7441 ctgcttttta ctttgagcta catcaaaaaa agttcaaaca tccttgatgc aaagcactat
     7501 atatagactt taaaatgcgt cccaacccaa tatgttgtat taatcgacta taattgctac
     7561 tacagctccc cacgaaaaag gtgcggcgtt gtggataagc ggatggcgat tgcggaaagc
     7621 accggaaaac gaaacgaaaa aaccggaaaa cgcctttccc aatttctgtg gataacctgt
     7681 tcttaaaaat atggagcgat catgacaccg catgtgatga aacgagacgg ctgcaaagtg
     7741 ccgtttaaat cagagcgcat caaagaagcg attctgcgtg cagctaaagc agcggaagtc
     7801 gatgatgccg attattgcgc cactgttgcc gcggttgtca gcgagcagat gcagggccgc
     7861 aaccaggtgg atatcaacga gatccagacc gcagttgaaa accagctgat gtcgggtcca
     7921 tacaaacaac tggctcgtgc ttacatcgag taccgtcacg atcgcgacat tgaacgtgaa
     7981 aaacgcggtc gcctgaacca ggagatccgt ggtctggtcg agcagaccaa cgcctcgtta
     8041 ctcaacgaaa acgccaacaa agacagcaag gtgattccaa cccagcgcga cctgctggcc
     8101 gggatcgtgg ctaaacacta tgcacgccag cacctgctgc cgcgtgacgt ggtgcaggca
     8161 catgagcgtg gcgatattca ctatcacgat ctcgattact cgccgttctt cccgatgttc
     8221 aactgcatgt tgatcgacct gaaaggcatg ctgacccagg gctttaaaat ggggaacgcc
     8281 gagattgaac cgccgaagtc gatctctacg gcaaccgcgg taactgcgca gattattgct
     8341 caggtcgcca gccatattta tggcggcacc accattaacc gtatcgatga agtgctggcg
     8401 ccgtttgtta ccgccagcta caacaaacat cgcaagaccg cagaagagtg gaacatcccg
     8461 gacgccgaag gctacgcaaa ctctcgtacc atcaaagagt gctacgatgc cttccagtcg
     8521 ctggagtacg aagtaaacac cctgcacacc gccaacggtc agacgccgtt tgtaaccttt
     8581 ggttttggtc tgggcaccag ctgggaatcg cgcctgattc aggaatcgat cctgcgtaac
     8641 cgtatcgcag gcctcggtaa aaaccgtaaa actgcggtgt tcccgaaact ggtgtttgcg
     8701 attcgcgatg gcctgaacca taaaaaaggc gatccgaact acgacatcaa acagctggcg
     8761 ctggagtgcg caagcaagcg catgtatccg gatatcctga actacgatca ggtagtgaaa
     8821 gtcaccggtt cgtttaaaac cccgatgggc tgccgcagct tcctcggcgt gtgggaaaat
     8881 gaaaacggcg agcagatcca cgatggtcgt aacaacctcg gcgtgatcag cctgaacctg
     8941 ccgcgtattg ctctggaagc aaaaggcgat gaagccacct tctggaagct gctggatgaa
     9001 cgtctggtgc tggcacgtaa ggcgctgatg acccgtatcg ctcgtctcga aggcgtgaaa
     9061 gcgcgcgtgg ccccgatcct ctatatggaa ggtgcttgtg gcgtgcgtct gaatgctgac
     9121 gatgatgttt ctgaaatctt caaaaacggt cgtgcgtcta tttcgctggg ttacatcggc
     9181 atccacgaaa ccattaacgc gctgttcggc ggcgagcatg tatatgacaa cgagcagctt
     9241 cgcgcgaaag gtatcgccat tgttgaacgt ctgcgtcagg cagtggatca gtggaaagaa
     9301 gaaacgggtt atggtttcag tctctacagc acgccgagtg aaaacctgtg cgaccgcttc
     9361 tgccgtctcg atactgctga gtttggcgtg gtgccgggcg tgaccgataa aggttactac
     9421 accaacagtt tccacctcga tgtggagaag aaggtgaacc cgtacgacaa gatcgacttt
     9481 gaagcgcctt acccgccgct ggcgaacggt ggtttcattt gctacggcga gtatccaaac
     9541 attcagcaca acctgaaggc gctggaagat gtctgggatt acagctatca gcatgtaccg
     9601 tattacggca ccaatacacc gattgatgag tgctacgagt gtggctttac cggtgagttc
     9661 gagtgcacca gcaaaggctt cacttgcccg aaatgtggta accatgacgc ctcccgtgtg
     9721 tcggtaactc gccgcgtgtg cggatattta ggtagcccgg atgcacgtcc gtttaacgct
     9781 ggtaagcagg aagaagttaa gcgccgcgtt aaacatttgg ggaatgggca gataggttaa
     9841 tcttcactct gcgctaagca aatgccggat gcggcgtaaa cgccttatcc ggcctacatt
     9901 tggcaacggc tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattaccgga
     9961 tgcagcctac acgcggactg ataagccagg cgaaataatg aattatcatc agtactatcc
    10021 tgtcgacatc gtcaacggcc ccggcactcg ctgcaccctg tttgtctccg ggtgtgttca
    10081 tgaatgcccc ggttgctata acaaaagcac atggcgggta aattctggtc agctatttac
    10141 caaagcaatg gaagaccaga tcattaacga tctgaatgac actcgtatca aacgccaggg
    10201 tatttccctc tccggcggcg atccgctgca tccgcaaaac gtgccggata ttctgaaact
    10261 ggtacaacgc atccgcgccg agtgtccggg taaagacatc tgggtgtgga caggctataa
    10321 actcgacgaa ctcaacgcta cgcaaatgca ggttgttgat ttgattaacg tgctggtcga
    10381 cggcaagttt gtgcaggatt taaaagaccc ttccctgatc tggcgcggca gcagcaatca
    10441 ggtggtgcat catttgcgat gataagttcc ctgttacctg agtgcaggca acagggaaat
    10501 gaggaattaa cgatacttct ggtgataggc gttgcgggtc gttttcagtt gctctgcggc
    10561 ggcctgcgct tcttttactt taccttcatt tgccagcttc agcgcgtcgt caatctgacc
    10621 gaccagaatg tcgaaaccgt ggcggaaatc tttcatttcc gggctgtccg gtgatttatc
    10681 ttcgagcttc ggcggcgttg ctttttgcgc atccagcgct gcggcgcgca tcatcgttaa
    10741 cgcgtctttg acttgcgccg cgttatccgc tttttcgatc acttttaaat tgtcgttgag
    10801 ggtttccata ttgtcttcaa gatcagcggc aaacgacgca gaactgaata ccaacgagga
    10861 gactgcaaga atagctaaca ggcttttacg cattgctcac ttcctttttt atttacactt
    10921 catccttcac actgcctcat cgacggctac gctcattcgc ccgggtcacg tactgcgagt
    10981 acgctccccg gccctcattc actttccgtc tcgatgcagt gcgaatgatt ttgtgtaatt
    11041 ttttagttta tagcgcggca ggtcgcgcca gttttttact gcccggcgat tttcatctcc
    11101 ggcaacagca cagaaccaca ctgtatatta ctgcgtgttt caatatcgtt accgacggtg
    11161 acaatattgc gccacatatc ttttaaatta cctgcgatgg tgatttcact aaccggatac
    11221 tgaatttcgc cattctctac ccagaagccc gcagcaccac gggaataatc accagtaatg
    11281 gcactcacgc cctggcccat cagctccgtc accaccagcc cggtgcccat ctctttgagc
    11341 atttgttcga agcttagccc ttgtccggca atccgccagt tgtgaatacc gcccgcatgt
    11401 ccggtgcttt tcagccccag tttccgcgcc gagtagctgg tcagcagcca ctgagtcagg
    11461 atgccatctt taataatatc gcgacgctcg gtgcgcacac cttcgctgtc gaatggcgtc
    11521 gacgccagcc ctttcagcag atgcggatgc tcttcaatgg tcagccagtc cggcagaatt
    11581 tgtttaccca gcgaatccag cagaaaggta gatttacggt aaaccgagcc acccgctatc
    11641 gcccccacca gatggccaaa aagcccggtt gccacttcat tggcaaaaat gactggcgct
    11701 ttcatggtgg agagtttacg cggtgacagg cgcgataaag tacggcgagc acagtcggcc
    11761 ccaacccact ctggcgtttg cagatcgctc atcgcacgac caatggtgta ggcgtaatca
    11821 cgctccatat cgccattttc ttcggcaatt acacagctgg agagtgaatg acgcgtagag
    11881 cagtaaccct gcaacatgcc gtggctgttg ccaaaaactt tgacaccgta gtggctgtta
    11941 aaactgccgc cttcggtatt ggtaatgcgt ttgtccgcct gcaatgccgc ctgttctgcg
    12001 cgggccgcca gttcaatggc atcatccggg gaaacttcag cagggtggaa caagtcgaga
    12061 tctggtgcgt caaaggccag cagctctttg tctgccaggc cggcacaggg atctggcgag
    12121 gtataacggg caatatccag cgccgcctgt acagtacggg caatggcctg cgggcttaaa
    12181 tcggtggatg atgcgctacc tttgcggttc tggtgataaa cagtgatccc cagcgcgcca
    12241 tcgctattga attcaacatt ctccacttca ccataacgcg tgcttacgct aatgccggtg
    12301 gtcttgctga cggcaacttc cgcaccgtcc gatttgcctg aggccaactc cagcgcagtg
    12361 gaaactgctt cttccagaat cttgcgctgc gcttcaactt gagagattac tttcattgca
    12421 agtgccataa tgtagagagt ttctctgaag tctaacagag aaccgttttt cagtgcgcat
    12481 cttaactggt aacattagcc tcttttttta aggagcctga gatgactaag cagcccgaag
    12541 actggctcga cgacgttccc ggtgatgaca tcgaagacga agacgatgaa attatctggg
    12601 tcagtaaaag tgaaattaaa cgtgatgccg aggagctaaa acgccttggc gcggaaattg
    12661 ttgatctggg gaaaaacgcg ctggataaga tcccgttaga tgcggatctg cgcgcggcta
    12721 ttgagctcgc ccagcgtatt aagatggaag gtcgccgccg ccagttgcag ctcatcggta
    12781 agatgctgcg ccagcgcgac gtagagccta ttcgtcaggc gctggataag ctgaaaaatc
    12841 gccacaatca gcaggtggtg ctgttccata aactggaaaa cctgcgcgat cgtctgatcg
    12901 atcaaggtga tgacgccatc gctgaagtat tgaatctgtg gcccgatgcc gatcgtcagc
    12961 agctgcgtac tctgatccgt aacgcgaaga aagagaaaga agggaataag ccgccgaaat
    13021 ccgcacgcca gattttccag tatctgcgcg aactggcgga gaatgaaggg taattttcgt
    13081 tgttgatgtg agtatggttt gccggatgcg gtgcacctgt aggcctgata agacgcatca
    13141 agcgtcgcat caggcactat gctcaaatgc cggatgcggc gtgaacgcct tatccggcct
    13201 acgcacacta tatcaggctg aggccgaatt actgcgcggc ttccgccttc ttcgccagac
    13261 catccagcag tttctgatgg atgccaccaa aaccgccgtt gctcatgacc agaatatggt
    13321 cgccaggctg agcggttttc accaccatat ctgccagcgt atccacatcg ccactccagt
    13381 gtgcaggctg aacgcaggct tctgccactt ctgccacctg ccacggaata tgcgccggtt
    13441 gcagcaggaa gacttcatcg gcacgaccta atgaaggtgc cagatcgtct ttgcagatcc
    13501 ccattttcat ggtatttgag cgcggttcca gcacagcaat aatgcgcgcc gtaccaccaa
    13561 ctttgccacg cagcgccgcc agcgttgcca gaatcgccgt cgggtgatgg gcaaaatcgt
    13621 catataccgt cacgccattc gcttcaccac gcaactccag acggcggcga gcgttaataa
    13681 acgaacccag cgcgttagcg gcatctgcag gcgctacacc aacatggcga gccgctgcaa
    13741 tcgccatcag gccattgtgc atattatgtt cgcctaccag cgaccatttc acttcgccca
    13801 ctttttcgcc atcaagcaag acttcccatt cggaagcatc ggtggtcagc tttttcgcct
    13861 gccagtgccc ctgctcaccc accagctcct gctcgctcca gcagcccatc gccatggtct
    13921 gtttcaggtt gatgtcattt tccggccaga taatacggcc ctgccccgga acgatacgca
    13981 ccagatggtg gaactgtttc tggatcgctt tcaggtcgtc aaagatatcg gcgtgatcga
    14041 actcaaggtt gttgaggatc agcgtacgcg ggcagtaatg gacaaattta gagcgtttgt
    14101 cgaagaaggc gcagtcgtac tcatccgctt cgataacaaa gaagtcgctt tcgcccagac
    14161 gcgccgaaac ctcaaagttc cccggcacac cgccgattac aaagcccggt ttgtaaccac
    14221 actgttccag aatccaggtc gccattcccg cggtggtggt tttgccgtgt gtacctgcaa
    14281 cggccagcac ccagcggtcg cgcagcacaa aatcgtgcag ccactgtgga cctgacatat
    14341 aagggatgtt tttttccagt accgcttcca cacacggatt tccacgggtc atggcgttgc
    14401 caataatcac cagatccggc tgcggatcga gctggctggc atcgtaaccc tgaatcagtt
    14461 caatgccttg cttctcaagt aaggtgctca tcggcggata cacattggcg tccgaacccg
    14521 ttacttcatg gcctaactgg cgcgccagca tcgccagacc gcccataaac gtgccacaaa
    14581 ttcctaaaat atgaatgcgc atacgtcact atcctttttt aatctgccgc tcattttacg
    14641 catatgtcgg gcaagtgaga aacgcatttc aggataatcc gcaatttgct ggcgcgattc
    14701 acctgagcgc aacattccga ttatttgtta agattgttgc ggtcgcttta ctccataaac
    14761 attgcaggga aagttttatg aaaacgttag gtgaatttat tgtcgaaaag cagcacgagt
    14821 tttctcatgc taccggtgag ctcactgctt tgctgtcggc aataaaactg ggcgccaaga
    14881 ttatccatcg cgatatcaac aaagcaggac tggttgatat cctgggtgcc agcggtgctg
    14941 agaacgtgca gggcgaggtt cagcaaaaac tcgacttgtt cgctaatgaa aaactgaaag
    15001 ccgcactgaa agcacgcgat atcgttgcgg gcattgcctc tgaagaagaa gatgagattg
    15061 tcgtctttga aggctgtgaa catgcaaaat acgtggtgct gatggaccca ctggatggct
    15121 cgtccaacat cgatgttaac gtctctgtcg gtaccatttt ctccatctac cgccgcgtta
    15181 cgcccgtcgg cacgccggta acggaagaag atttcctcca gcctggtaac aaacaggttg
    15241 cggcaggtta cgtggtatac ggctcctcta ccatgctggt ttacaccacc ggatgcggtg
    15301 ttcacgcctt tacttacgat ccttcgctcg gcgttttctg cctgtgccag gaacggatgc
    15361 gcttcccgga gaaaggcaaa acctactcca tcaacgaagg aaactacatt aagtttccga
    15421 acggggtgaa aaagtacatt aaattctgcc aggaagaaga taagtccacc aaccgcccgt
    15481 atacctcacg ttatatcggt tcactggtcg cggatttcca ccgtaacctg ctgaaaggcg
    15541 gtatttatct ctacccaagc accgccagcc acccggacgg taaactgcgt ttactgtatg
    15601 agtgcaaccc gatggcattc ctggcggaac aagcgggcgg taaagcgagc gatggcaaag
    15661 agcgtattct ggatatcatc ccggaaaccc tgcaccagcg ccgttcattc tttgtcggca
    15721 acgaccatat ggttgaagat gtcgaacgct ttatccgtga attcccggac gcgtaatctc
    15781 ctgcgcctct ccacacggag aggcgttttt atcgctgcgc aatgttgact cttgtcactg
    15841 gcgaactctg acgattctcc cacagcaccg ttaatccacg ctgtaatgct ataaaaataa
    15901 acaacaaaat accgatggcg attttcgtcc accatgagct cagcgtgcca tcaaagttta
    15961 tgtaagtctg aatcagtccc tgaatcgcca cgccaaaaag cgtccctaat accgttccaa
    16021 cgccaccgct caaaagcgta ccgccaatta ccactgaggc gatagcatcc agttccacac
    16081 ctacgcccgc cagcgcatat ccggcctggg tataaatcga gaagacaatc cccgccagcg
    16141 ttgccagtcc ggtggagagc atataaatgc gaatagtggt gctgcgggta gaaatcccca
    16201 tcaggtttgc cgacgttgcg ttgccgccaa tggcgtatac ctgattacca aaacgggtac
    16261 gatgcgcgag gaatatgccg ataaccacca ccgccagcat cagcagtccc atcgcactta
    16321 agcgaccgcc gccagggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt
    16381 ttatcggaat cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacattcctg
    16441 ccaaagtaat gataaacgcc gggatcttca gggcgtcgat caaaagcccc ataaatgcgc
    16501 caaaggcaca gcccattacc agcaccagcg gaaatgccag caacggcgag aggccgaagt
    16561 cgccaatcac ttttgccaga aacacgccgg taaaagcgat caccgaaccg acggagagat
    16621 cgatcccacc ggagaggatc acaaaggtca tgccaacggc aatgatccca agaaaggcgt
    16681 tatcggtcag gatattgcag atcactcttg tggaagcaaa accgggaaac tgcgtcaggc
    16741 agtagagata gcccagcaca aagacgccga tggtgatcat cagtggcaaa ttacgtttta
    16801 tcacggctac gtactccttt aatcagactg ataaagcgtt gcgactggac aatcagcaca
    16861 caaagcacca ccaccgcttt tacgacctgg ttcatctccg gcggaaagcc agaaagtaaa
    16921 attccggtat tcattccctg aataatcagc gcccccacca ccgaaagtag caggttaaag
    16981 cgcccgccca tcagcgatcc gccgccaatc accaccgcga gaatggcgtc cagctccagc
    17041 cataacccgg cgttattggc atcggcaccg cgaatatccg ccgccacgat aatgcccgca
    17101 atcgccgcac atagcccgct caacacgtag gtgagcatga cgatgattcg cgtgtttacc
    17161 ccggcatttt ttgccgcccg aatgttgata ccaacggctt cgataaacat ccccagcgcc
    17221 gttttgcggg tcaacagcca gaacaggaga agcgtcagca ccgcgataat gaccggcgtt
    17281 ggcaggaaca acagcgatcc gctgccaaac catgagagat ccggcgagtt aaacgtgacg
    17341 atctggccgg aggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg
    17401 gcgacaaacg gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg
    17461 atgccggtgc ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc
    17521 gcagccgttg tggctccggc gatagccatc accgccccta cggagagatc aatcccaccg
    17581 gtggcgatca ccagcgtcat accaatcgcc agtaacgcaa cgggagctgc acggttaaga
    17641 atgtcgatag ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg
    17701 gctaccaggc tatcgaccag cagcaccagc aatagcgccg ccagctgcga cattcccgtt
    17761 ggccagcgaa agcgcctttt cggcggcgta gtgtccggga gagattgagg catcacactg
    17821 ttctccttac gccgcaatgg cgttcatgat cgccggaacg gaaagctctg ccagcgggat
    17881 ctccgccacc tgtttgcgat cgcgcatgat aatcacccga tcggcatagc ccaccagctc
    17941 ttccagttca gaggagatca ccagcagcgc cagaccatcg gcgcatagcg tttcaatcag
    18001 gcggatgatc tcggcgtggg cacccacatc aatgccgcgg gttggctcat cgaggatcag
    18061 aaattgcggt cgggtcagta gccaacgtga aagcaacact ttttgctgat tgccgccaga
    18121 gagaaattca atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg
    18181 ttcggcaatc tcttgctgct ctttgcggga aatgggtcgt agccagccgc gctgggcctg
    18241 gagagcgagg atgatatttt cccgcaccga ggcggcggcg atgatgccat cggttttcct
    18301 gtcttccggg cagaatccga tgcccagtac tgaagcctga tgtggcgatc gcagggtttg
    18361 cggtttgcct ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc
    18421 ggcggtttcg gtacgtccgg atcccagcaa gccagccaga ccgacgatct cgccggggcg
    18481 cacttcgaga tcaaacggtg ctatcgttcc ttttttgccg taatttttga acgcggcaac
    18541 gggtttgtcg ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg
    18601 ccccagcatc atttttacca gttcgatctg cggtagctcg cgcgtttccc gacagcctac
    18661 gaaactgccg ttgcgtaaga cagtgatccg atcgctgacc tgatagacct gatcgagaaa
    18721 gtgagtgaca aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggccaaataa
    18781 tagctccact tcctgggtgt cgaggctggc ggtgggttca tcgaggatca gcactttggc
    18841 ggagagatcg atagcccggc aaatggcgac gatttgctgc atcgcgactg aaaagcggtt
    18901 aagcggttcg cgcacgtcga ggaagaaacc gtaagatgcc atcagttcgg tggcgcgctt
    18961 ttccatctct ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt
    19021 atcagcaacc gacatattgg gtagcaggtt gacttcctga tagacggtgc cgataccgag
    19081 ttgttgtgca tgggcggtat ttttcggtga gatagcctgg ccttccagcc agatggtgcc
    19141 gcgatcggcg tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt
    19201 ttcaccaagc agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc
    19261 tttgacgccg gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc
    19321 ggtcgtcatg gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta
    19381 agcgtcgcat caggcttcag cgcaccaccg ccggatgcgg cgcgagcgcc ttatccggcc
    19441 tacgaatcgg catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact
    19501 cttcttttgc ggtatcaggc agataaaggg tggatttggt cagggtcagc ttttcaggca
    19561 tggtgccgtc ttttttgtat ttctccagcg catcgaaagc gggacctgcc atattcggcg
    19621 tcagttcaac actggcgttc gcttcgccat caatcatcgc tttgtagata tccggtacac
    19681 cgtcaatgga acccgtgagg atatctttgc ccggtttcag gcccgcttct ttaattgcct
    19741 gaattgcgcc aatcaccatg tcatcgttat gggcgtaaac catgcagatg tttttgccgt
    19801 tgttttccgc tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac
    19861 ctgactgcga gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt
    19921 tcttacggtc aatggcgacg ctggccccaa cggtgccctg caactccacc acgttgcatg
    19981 gtttgccatt cacttctttt accagccagt caccgatcaa cttaccttcg aggatgttgt
    20041 cggcagtaac ggtggtcata tagagcgatt tgtctttcac atcgatggaa cggtcgagca
    20101 agaagaccgg gatttcggca tctttcgcct cttttaatac cggttcccaa cctgtcgcga
    20161 ccaccggagc gataaagatc gcatccaccc cttgtgcaac gaaggaacgt accgctttaa
    20221 tctggttttc ctgcttttgc tgaccatcgg caattttcag cgtaattccg cgtttttcgg
    20281 cctcactttt cgccacattg gtttctgcgg cgcgccagcc ggattccgat ccgacctgcg
    20341 aaaatcctac ggttaatgga gcggccaacg ccatagacga catggctgcc gagactgcag
    20401 agactacaag taaacgtttc cacatagggt tgtcctcgtc ggggtagtta ttattggtta
    20461 aaagatgttc gcgaaaaaac tatagacaat cggtgatgta acgaattgcg ttacatcaca
    20521 cttcaaaaaa gtaaataaaa cgttaatcac aagtttgtaa tcgctttcat ctcactatga
    20581 aaaatgcggc tacggttatg gattttcctg ctctgcatac cgtcttaaaa ctggcgaaaa
    20641 aggaaaatga agacgaaaac aagcgaagac attcggcgcg agttggctat aatactcggc
    20701 acttgtttgc cacatatttt taaaggaaac agacatgagc ttactcaacg tccctgcggg
    20761 taaagatctg ccggaagaca tctacgtggt tattgagatc ccggctaacg cagatccgat
    20821 caaatacgaa atcgacaaag agagcggcgc actgttcgtt gaccgcttca tgtccaccgc
    20881 gatgttctat ccgtgcaact acggttacat caaccacacc ctgtctctgg acggtgaccc
    20941 ggttgacgta ctggtcccga ctccgtaccc gctgcagccg ggttctgtga tccgttgccg
    21001 tccggttggc gttctgaaaa tgaccgacga agccggtgaa gatgcgaaac tgattgctgt
    21061 tccgcacacc aagctgagca aagaatacga tcacattaaa gacgttaacg atctgccaga
    21121 gctgctgaaa gcgcagatcg ctcacttctt cgagcactac aaagacctcg aaaaaggcaa
    21181 gtgggtgaaa gttgaaggtt gggaaaacgc agaagccgct aaagctgaaa tcgttgcctc
    21241 cttcgagcgc gcaaagaata aataagttct tctggcgcaa taaccctgaa caccgggctt
    21301 cggttagtaa gggttttttt atgcccgcga taaataaaca caccttattc caccaccgcc
    21361 tgcaagcgta ataacgcctc ttccaccacc tcatccgcag ccagaccaat acgctttgcc
    21421 agccgggcgc gtagatccat catccgcacc tgattcacca gcaccacgcc gtgcacatcg
    21481 ccttcttcgc aatgtaaagg aacgctaaat ccggcataac gggcaaaatt tccgccctgc
    21541 gtaatggggg ccaccagcgt cattcccagt tgattaaagg cttgaacgga gagcacaagc
    21601 gcaggtcgac cagcaccttg ctgttcatgg ccgcttgctg gatcaaagcc aaccagcaca
    21661 atgtctcccc gttcaaattc acttttcttt accatatttc gtcacccgca ggggtggatt
    21721 taccccagac atcctgctcg ctaagttccg cggcgttcat gtcacactgt gccagcagtt
    21781 catcaagcga gtagcgcctg gagatgggtg tcagaatcag ttggttgttg ctcacctgcg
    21841 cttccacgct ctgccccggc tgtaagttaa gttctttcat tacgatattg ggaatgacca
    21901 tacctgcact gttcccccat ctttttatgg taatacgcat ctgcacctcc ttaagttata
    21961 caaagtataa ctcacggtgc tacaggaaac gttttacccc tgcacatttg tgaacgccag
    22021 cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag caaaagatgg
    22081 aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa caacgccacc cgaaggtggc
    22141 gtgcttttat tattacttat ccctgtctaa ccagtcgccg ctttcaatta gctttaatcc
    22201 atcgacgggt cgttgataaa cgtacatcca tgcactcccg tacggcgtct gaatcaactg
    22261 gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt tcggccagcg tggcgttgtc
    22321 aatacgataa acttcaccgt gtactgttcc gttccccgga actgcgcctg gatagtggcc
    22381 caggctatac aactggtagt tatcgatact gaaatcgccc agtaactggg cattggtcat
    22441 ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag acaaatattc gcattgctaa
    22501 aactcgaact gatagagcaa atccagtgcc tggtctacac cagacacggc ttccagatat
    22561 agcttaggca tcaggcgata acgtaacgtg agtgttgcta tagagtcaaa tatacccacg
    22621 ccgtatttca cttgcagacc tggcaataca tagccgctga ccactacctg ggaggagtcg
    22681 cctactccct gggtgtcgag cgctaaattg cttacgccaa acgtctcgcc gattttaccc
    22741 acaatctggc cactttgcgc aacccccagg ccaatcagca ttgaggtcat cgccgcactg
    22801 tcgctctgat cgctctccag cccttgtcca cgtagcaaat aagacaatgc agcttgttgc
    22861 gacatagccg ggtcagagaa gatctccgct ttcggttcgt ccgccagacc agtgacgcga
    22921 actccggcga ttacgtcgtc ttctgtagca tccgggttac gaatagcttc aatattaaga
    22981 tacggttgat ctggcggacc agagaacagt agctcaccct tacgcacaat cagatcctga
    23041 ccataggcat ggaagcgccc ttcagggatg ttgatctgcc cgttcaggcc cagcccttgt
    23101 ttgtcctgaa cgacgttaag atcgcccgtc agccgcgctt tcaggccaaa ggcgtcaatg
    23161 cgcacattgt tgccaacgtg gacaatcagg ttactgttaa tcggaatcga cgccgttttc
    23221 ggctcttccg gttgcaggtt atcgttaagc atcaccacat cgctggagac gcctactgcg
    23281 ctttccggca gatcgtgcac cacgatgcgc gcccacggga catccacgcg accatcgagg
    23341 gtaaacaggt ttggtgtagc ctcgaataca acatctggcg atacatccat tcgtaccatc
    23401 ggcggcacgg tgatccgcac tttactgcct ttcgccgtta ctcgtgcccg ccagttttca
    23461 atttggctcc agtcggcatc accgttcagg tagatttcac cctgctgggt ccgtacggta
    23521 ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa gctggctcgg ctgcatatca
    23581 aacggcataa agttgccgtc gatatccaca cccgtaacct gaagctgacc aaacaactgc
    23641 gggctttgca catcaccacc cagacgcaag ttggcactca ccatccccgc tgctttttcc
    23701 ccacgggtaa agatggggtt tatcatcgca aggttgaagt tacggatatt gacgttgcca
    23761 ccaagattac ggcggccttg cggatcggtc acctgcacct gtccatcaaa ctggccgtta
    23821 ttggtcaggc ggatggtcca gcccaattcg gcacggttgt tacgcaattc cgccgtcaga
    23881 ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga cggtttgcgt tacctgcacg
    23941 ttacgccccg aaagggtgat actgccctgc ggcagcccct ctttcgtggt gtcccaggca
    24001 acatccgctt tacccgtgaa gataccgctg gcctgagtgg tttctggcat aaacggtttc
    24061 agcatggcga ggtcgaagcg gttgagattc accaccgcac gcccttcggc ccccgcatcg
    24121 atagtttgcg gcacgcacag ttccgcattc gggttaagcc agcagtgtgg cccgatgctg
    24181 attttttgct ccttattgcg gtaatccagc gcaatatcgc gggtcagcga ccacgggccg
    24241 accggcgtct ggaagcgggt attgctaaga gttcccttcc agcgttcttc tttgcgatca
    24301 aaacttcctg ccagattaag ctgcccggaa accggctcgc cctgaatccg caactgtagc
    24361 tcgtgctgct tttcgctgcc tttggcattc agggtgacga ggttgatatt tacatccggc
    24421 tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga tctgatcggt ggatttaatg
    24481 tcgccttcca cgcgaacctg cgtcacggaa agttcctgcc agcgcaggcc gcgcgcggtg
    24541 atatctgcca gtagttgtgg cgcttccacc gtgccgcgta ctttcaccag ccctttcgct
    24601 gtaccgccaa gccccggcag cgcgttatcc agccccggcg cgttgatggt ggcatcaaga
    24661 ttgagatctt ttacccccag ctcgcctttc acttcggcac tgtttggccc cagttccaga
    24721 tgaagccctg ggatcatcca ctgcatataa ctgttgcctt tcagcgtgcc gtcaacgttc
    24781 actttgttct gtttgacgtt accggtcagc ttcagttctg gcacctccat ctgccaggtg
    24841 ccgccgtaca ggctaccgcg ggttttaatc aagccattga gtttcgacgg ccactccggg
    24901 atctctttgg cggtgttaat gccgttaagc gttagctcac cgcgccaact aatggcctgc
    24961 tgccagtcga gcaacgcctt gagttcagtt ttcccttcca gcgccgcgac ggtgagtttg
    25021 tcgagattga cctgctgttc attacctttg gcatcaaggg taatggtggc tggcgggatc
    25081 tccagtccct tcactgccgt acgcatagag agcgtgtaat cggtcatttt gccggtaagt
    25141 ttcagtttca gatcatccgc ctgatactgc ttctcaccgg tgaacggcca gtaaagctgt
    25201 ttgctgttca cttccacgtt gagtggcaat ccggcttccg ccagtcgcgt ctgggcgcgt
    25261 aaatccatat ccaccggacc ggaaaggtta acgccaatct ccagctgttc gcgcagcgca
    25321 ccgcccactt tcagcttcag tttttctcct ttcaacggct ccacgtttag tgtgcagttg
    25381 agggtgatat ccaccggcca gttgtctgac agctgcgccg tgccgctggc gttgacgatc
    25441 ccttgactgg aatcgatatc cagggcgtcc agtttagtat tgccgtcaat gctgctcact
    25501 ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg tcacgcgtaa ctgctcgcct
    25561 ttaaactctt caatgttcag gttaagcggc aaatgcacgt cggtcatttc cggcaatacc
    25621 gggcgagaaa agagatcttt cagcgtttcg ccgagcggct tttcctccgg ctgcggattt
    25681 tcaattttcg gttcgaccac ttcttcctgc gccacttccg ccactttcgg cagagcaatc
    25741 agcaggcctt tcagcgacgt cggtttcagg gtcagggttt tctcctgcca gttcaggccg
    25801 gaggtgaagt ccatcaccga caccgtggtg tcatcaatct tgatgttgac gttgtccagc
    25861 gccacccgtg tcagggtgat gggatacggc gtggagagat ccagcggacc gctatcttcc
    25921 tcttcttcaa cctgttcaga aggaggcatt tttttactgt cgatgttgac ctgaatgtct
    25981 ttcagcgcca ggtcattaat acaaacgcta ctgttccaca ggcactcaag accgaccgcc
    26041 agatgcagat tacccgcttt taccgccacg cctggctgct cataacgaac gtcagacaag
    26101 gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca gtcctggcac ccagcgttcc
    26161 gccgctttaa atacaagatg caggccgctg gtggtgccca ccagaaacgc caccgatccc
    26221 agcaacagta agataacgat aaccacgccg aggctgattt ttttccataa actcataatt
    26281 ctggccccag accgatgtaa aactgtaacc cgtgttcgtc tttatccgcg accggtacgg
    26341 caaaatcgag tttgattggc ccgaccggcg attcccagcg cacgccgacc ccggtaccgg
    26401 ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc actatcgaca aacaccgcgc
    26461 cccaccattt tccggtcacg ttgtactggt attccagcga tccggttatc aacttcgagg
    26521 cccctttcag gtcaccgttg gcgtatttcg gggcgataga tttgtatttg tagccgcgaa
    26581 tactgcgatc gcccccggcg aagaaacgca gatccggcgg tactttgtcg aaatcaccgg
    26641 tttcaatcca gcccagcgtg ccgcgtgtaa cgaaacgatg gcgatcgtac agtgtgcgga
    26701 tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc tgaaccccag gccgtgttgg
    26761 agtagtcgat agagtagcgt tgcgagtcgc cccaggttgg catcaggcca ccacgagaac
    26821 gcgtgcggct aatcatcacc ccaggataaa acagcatcgt ggtgttggta atttcaccct
    26881 gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg ctgccagccg ctggagagat
    26941 cccagtagcg agaagccacc agcgtagtgg agtcagattc ggtatcgttc aggtcagtgc
    27001 gcttaaaacc gccctgcacc aaataatatt gttccagtgg attcttcagc agcggcattt
    27061 tatagctgaa gtcgagggtc tgttccggcg cggaaatact ggtactggtg gtcagactgt
    27121 gaccataaga gttcatccac ggctttttcc acgtcgcttt cacgcgcggt cccacgtccg
    27181 tagagtaacc gaccccggtt tcgatggtgt tttcagttcg cggcgaaacc acgcccgtca
    27241 agggtaatac tttcgtttcg cgcgctttat caaattgtgg agccaccacc accgagttaa
    27301 accagccggt agcagaaagt cggcggttca gttccgccag atctttcgat tcgtactcat
    27361 cgccctcttt aaacggcacc agattttgca ggtattcatc gcggatttgt gatccttcaa
    27421 aggtcacatg cccaaagcgg taacgttcgc cactgttata atcaatatcc cagaaggctt
    27481 tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa ttcgctatcg aaataacctt
    27541 tacgcaacgc aatgctggtt aaggactttt tgaaattttc ataatcgccc tggttcagca
    27601 ccgtgccaat agccgggcga gtatcgagca atttcaaata gtctttatcg gtccgcgcgc
    27661 cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg cacacctggc gtgactttgg
    27721 cgatcaatac ctgccgccct ttctttggcg gtggacggag atcaaattca atggtcggct
    27781 ggtaataacc cagcgctttc agaccttcgc ggatggcatc atcgacgcgt gcgcgaaagc
    27841 gacggtctgg cgtcacttca tcactttcaa tcgtagaaag ctgcgcacga acgttctttt
    27901 ccagctgtcc cgataacccc tcgacctgta gacggacgtt cgcggcgacg gcagatccgc
    27961 ttaagcagag taagcttaca cagcataact gtcggatata gcgcacattt tctcctgaat
    28021 atcctttttt cctgcccctg gaacgccgtt aaacggctta acaaaaatcc agtaatatgg
    28081 attaaaaagc agactaaacc ccaaatattt cttatgtttt actttagacc tattcacggt
    28141 ggttattgtg tgcaaatacg cctcttgtta caaccttaac cccaatgacc gattttcggg
    28201 agagcgacac catgagttta tttgataaaa agcatctggt ttcccccgcc gatgccctgc
    28261 ctggacgtaa caccccgatg cccgtagcca cgctgcatgc ggtcaacggt cactcaatga
    28321 ccaatgtacc tgacggaatg gagattgcca tttttgcgat gggttgtttc tggggagtgg
    28381 agcgtctgtt ctggcagtta cccggcgttt acagcaccgc cgcaggctat accggaggct
    28441 atacgccaaa tccgacttat cgggaagtgt gctccggtga tacgggtcat gccgaagcgg
    28501 tacgcattgt ttacgatcct tccgtcatca gctatgagca gttgctacag gtattttggg
    28561 agaatcacga tcccgcccag ggaatgcgtc agggcaatga ccacggcacg cagtatcgtt
    28621 cagcgattta tccgctgacc ccagaacagg atgccgcagc tcgcgccagt ctggaacgtt
    28681 ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat caccacggaa atcgctaacg
    28741 ccacaccgtt ttattatgcc gaagatgacc accagcaata tctgcataaa aacccgtatg
    28801 gttactgtgg aattggcgga attggcgtct gtctgccacc ggaagcatag cgttacgggt
    28861 acaaatgtag attgttgata aagtgctctt tgtctatgcc ggatgcggcg tgaatgcctt
    28921 accaggccta caaaatcgtc caaattcaat atattgcagg gactgcgtag gcctgatcgg
    28981 catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc tctggcgact
    29041 ttacagtacc ttacgctata ctagccactg aaaatgccgg ttcactttct tcgaatcggc
    29101 tttcaatgtg tatttcacac aaattaatca acttcccctt ccgaggatct ggcctgaaag
    29161 gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc tgtaagtgcg
    29221 ttcttctcga tgtctgagat ctcacttgcc gcctcacgca aaatcaaact taaactgctg
    29281 gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa tcccggcatg
    29341 ttctttacgg tggtccaaat cggtctgaac gcagttgcga ttctcggcgg tatcgtcggt
    29401 gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc ggcagaactc
    29461 tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt tatcctgttt
    29521 gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc tttgcgtatc
    29581 atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc tggtgtggtt cttcaacggc
    29641 ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga tgacatcact
    29701 tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt acgtaaacag
    29761 gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc gtcttcaatg
    29821 acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag cctgaagaat
    29881 aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat tgaccacatc
    29941 atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca aagcctggca
    30001 ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt aaccctttca
    30061 gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat gaacgagtac
    30121 gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat gggcgatctg
    30181 gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg gctgattgac
    30241 ggcggcaccc caattgacga cgtcatgcgc gtgctggata ttgacgagtt cccgcagtcg
    30301 ggcaactacg aaaccatcgg aggctttatg atgtttatgc tgcgtaagat cccgaaacgc
    30361 accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga taactaccgc
    30421 atcgaccagt tgctggtgac ccggatcgac agcaaggcca ccgccctttc gccaaaactg
    30481 cctgacgcta aagataaaga agaaagcgtc gcgtaacccc cagaaacatc aacggctcct
    30541 gaatcaggag ccgtcttatt actgcatagc actttggtta agccatctct gtttgcagac
    30601 gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc ttcacttttg
    30661 ggataagaat cttaccctta tcaaattcaa aagcgccaac gtccttgatg tataaccgtc
    30721 cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt tggaaaattt
    30781 tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc ccaactatta
    30841 tgaggcgcaa attttaatgc ctagtgacta tagactatcc gggcaatgtt tccaccgcgt
    30901 ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc gaagcgcggt
    30961 cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa ctggcatcac
    31021 acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa gtggtcgtat
    31081 cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg cactcacctg
    31141 cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa accggtcagc gagtgccgcc
    31201 gattggcatc accgatcgtg gcgagttggt gcttgataaa gatcagttta gctacaaaac
    31261 ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg ctggtcgcac
    31321 ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga agttaccgca
    31381 cgatcgttac cagaccacca ccattgttaa caccgacgac gcaattccgg gttccggcat
    31441 gtttgtgcgc agcagtctgg agagtaataa aaagctttat ccctggtcgc agtttattgt
    31501 tgatagcaat ggcgtcgcac gcggtgcctg gcagctggat gaaaaaagtt ccgctgtagt
    31561 ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctta ctcaggaaga
    31621 ggtgcagcaa gtgatggacc tgctgcataa attaattaat aaatagacac tctgaaccca
    31681 ggattcagga acgactcgcg cggagtgtaa tccagcggtt taccctgcca gtcgtgaacg
    31741 tgcgctccgg cagctgcggc cacagcatgt ccagcggcgg tgtcccaaat attcgttggt
    31801 ccgaagcgcg ggtacagctg cgcctgtcct tccgccacca ggcagaattt cagcgaagag
    31861 ccgatggacg tggtctgatg ttcgccaagc tgttgcagat actctttcag ctccgcatct
    31921 gcatgggaac ggctgataac caccagcggc gggcgcgcat cgcggacctg aatctgcttg
    31981 cgcacaccgc actcttcttt ccaggccttg ccttctgccg cgctgtacat tacgttcatt
    32041 accggcgcat acaccacacc taaaatcggt ttgccatggt caatgagcgc aatgttaacg
    32101 gtgaattcgc cattgcgttt aataaactct ttagtaccat ccagcgggtc taccagccag
    32161 taacgctgcc agtgctgacg gacttcccaa ccgggaggat cttcttcaga aaggaccgga
    32221 atatccggtg tcagcgtacg taaaccgtcc atgataacgg tgtgtgcggc aatatccgct
    32281 gccgttaccg gagaattgtc cgctttgctg acgacgtcca tcggtttcgt cccgtcgtag
    32341 acctgcataa tggcatcgcc tgcattccgt gcaagctggc atacttgatc taacatttct
    32401 ccacctcgtc tctgtgagcg gtgttaactt attgttttac ttatacccta tcgttaatga
    32461 atgcgccaac tgtgatagtg tcatcatttt caaagcgtaa aattgtggca ttcttcactg
    32521 ttctataagt aagacgttta ttctcccttt tctttcgtat tcccgatgat aaaaggatgt
    32581 ccctgatgat taagtttagc gcaacgctcc tggccacgct gattgccgcc agtgtgaatg
    32641 cagcgacggt cgatctgcgt atcatggaaa ccactgatct gcatagcaac atgatggatt
    32701 tcgattatta caaagacacc gccacggaaa aattcggact ggtacgtacg gcaagcctga
    32761 ttaacgatgc ccgcaatgaa gtgaaaaaca gcgtactggt cgataacggc gatttgattc
    32821 aggggagtcc gctggccgat tacatgtcgg cgaaaggatt aaaagcaggt gatgttcatc
    32881 cggtttataa ggcgctgaat acgctggatt atacggtcgg tacactcggc aatcatgaat
    32941 ttaactacgg cctggattac ctgaaaaatg cgctggcggg agcgaaattc ccttatgtaa
    33001 atgccaacgt cattgacgcc agaaccaaac agccaatgtt tacaccgtat ttaattaaag
    33061 ataccgaagt ggtcgataaa gacggaaaaa aacagacgct gaagattggc tatattggcg
    33121 tcgtgccgcc gcaaatcatg ggctgggata aagctaattt atccggaaaa gtgacggtga
    33181 atgatattac cgaaaccgtg cgcaaatacg tgcctgaaat gcgcgagaaa ggtgccgatg
    33241 tcgttgtcgt tctggcgcat tccgggctgt ctgccgatcc gtataaagta atggcggaaa
    33301 actcagttta ttacctcagt gaaattccgg gcgttaacgc cattatgttt ggccatgctc
    33361 acgccgtttt cccgggtaaa gattttgctg atatcgaagg ggctgatatc accaaaggca
    33421 cgctgaatgg tgttccggcg gtaatgccgg gcatgtgggg cgatcatctt ggtgtggtcg
    33481 acttacaact cagtaatgac agcggtaaat ggcaggtgac gcaggcgaaa gcggaagcac
    33541 gaccgattta cgacatcgct aataaaaaat ccctcgcggc ggaagacagc aagctggtag
    33601 aaacactcaa agccgatcac gatgccacac gccagttcgt cagcaagcca atcggtaaat
    33661 ctgccgacaa tatgtatagc tatctggcac tggtgcagga cgatccgacc gtgcaggtgg
    33721 tgaacaacgc gcaaaaagcg tatgtcgaac attacattca gggcgatccg gatctggcaa
    33781 aactgccggt gctttcagct gccgcaccgt ttaaagttgg tggtcgcaaa aatgacccgg
    33841 caagctatgt ggaggtggaa aaaggccagt tgaccttccg taatgccgcc gatctttatc
    33901 tctaccccaa tacactgatt gtggtgaaag ccagcggtaa agaggtgaaa gagtggctgg
    33961 agtgctctgc cggacagttt aaccagattg atcctaacag cacgaaacca cagtcactca
    34021 ttaactggga tggtttccgc acttataact ttgatgtgat tgatggtgtg aattatcaga
    34081 ttgatgttac ccagcccgcc cgttatgacg gcgagtgcca gatgattaat gccaatgcgg
    34141 aaaggattaa gaacctgacc tttaacggca agccgattga tccgaacgcc atgttcctcg
    34201 ttgccaccaa taactatcgc gcttacggcg gcaaatttgc cggtacgggc gacagccata
    34261 tcgcttttgc ttcaccggat gagaaccgct cggtgctggc agcgtggatt gctgatgagt
    34321 cgaaacgtgc gggggaaatt cacccggcgg cagataacaa ctggcgttta gcaccgattg
    34381 ctggcgataa gaaactggat atccgtttcg aaacttcccc gtcagataaa gccgcagcgt
    34441 ttattaaaga gaaagggcaa tatccgatga ataaagtcgc gaccgatgat atcgggtttg
    34501 cgatttatca ggtggatttg agtaagtaaa acacttcttt ttcggcctat aaatcatcaa
    34561 ccgcatccgg catttattgg cggatgcgat gctggcgcat attatccgcc ctacaagcca
    34621 tgcaccgtag accagataag ctcagcgcat ccggcagtta tgccgcacgt tcatcccgca
    34681 ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc cagtgcagca accttttcgc
    34741 tcacctgctc gcccagcggc gtgaggctgt attcaacatg cggtggcacc accggatacg
    34801 cgatacggtt aagaaaacca tcctgttcta acgcctgtaa cgactgcgca agcatctttt
    34861 cgctcacccc gccgattttg cgccgcaggt cgctaaagcg atgagtacct tcgcgtagcg
    34921 ccaccagaat caacaccccc caacggctgg tgacgtgttt caacacctcg cgcgacgggc
    34981 actgttccgc aaagagatta ccctctttca gttgttgcga caggctaacc tgactcattt
    35041 catacttacc tttttgtacg tacttactaa aagtaagttt aggtgttagc gtatttaaac
    35101 tcaagacaaa acgatggaga cttcccatga tcgctattac tggtgccact ggccaacttg
    35161 gtcactatgt tattgaatcc ttgatgaaaa cggttcctgc cagccaaata gtggctatcg
    35221 ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg cattaccgtg cgtcaggctg
    35281 actacggcga tgaagccgca ctgacatctg cacttcaggg agtggaaaaa ctactgctga
    35341 tctcttccag cgaagtgggc cagcgtgccc cgcagcatcg caatgtgatt aacgcggcga
    35401 aggcggcggg cgtgaaattt atcgcttata ccagtctgct tcacgcggat aaatctccgc
    35461 tcggcctcgc cgatgagcac atcgagacgg agaaaatgct ggctgattct ggcatcgttt
    35521 acaccctgct gcgcaacggc tggtacagcg aaaactacct cgccagcgca ccggcagcac
    35581 tggagcacgg cgtatttatc ggtgccgcgg gcgatggcaa aattgcctca gcaacgcggg
    35641 cagattatgc ggcagctgcg gcacgcgtga ttagcgaagc cggtcacgaa ggcaaggttt
    35701 acgaactggc gggcgatagt gcctggacgt tgacacagtt agcggcagag ctgaccaaac
    35761 agagcggcaa acaggttacc tatcaaaatc tgagcgaagc cgatttcgcc gcggcactga
    35821 aaagcgtcgg actgcccgac ggactggcgg atatgctggc ggattctgac gttggcgcat
    35881 cgaaaggcgg tctgtttgat gacagcaaaa cgcttagcaa attgattggc cgcccaacga
    35941 caacgttagc cgaaagcgta agccatcttt ttaatgttaa taactagtta attaaagtgg
    36001 catcctcccg catcctctct gataatgacg ggatgccggg agcaatcatg tctgcttcct
    36061 gaacgtttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    36121 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgtg ccggaatacc
    36181 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctacccattg
    36241 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    36301 tgctcactat gatgggcaac ctcatttatt acttctgcct tgccagtgcc attcaacgta
    36361 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtcatt cctgtctttg
    36421 ccaatctgct ttatagccag cgcgacggca aactcgcgtg gggaaaactc gccccggcac
    36481 tgatttgtat tggcatcggc ctcgcgagtg tgaatattgc tgagttaaac cacggactcc
    36541 ccgattttga ctgggcacgt tatacctctg gcatcgtgct agcgttagtt tccgtggtct
    36601 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    36661 cgatgatgtg ggcaacagcg caggcgctgg tcacactgcc ggtttcactt atcggctatc
    36721 tcgtcgcttg ttactggctg aatacgcaaa cgccggactt ctccctaccc tttggccccc
    36781 gtccgctggt gtttattagt ctgatgattg cgatagccgt gctttgctca tgggttggcg
    36841 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    36901 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    36961 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcatcgcg gtcagagcaa
    37021 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    37081 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    37141 ttatcttatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    37201 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    37261 tggcggtaag cagacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    37321 tgaagctgaa ctggcaaagc tcgctgaaca accgattgag aaagactggc gtagcgcccc
    37381 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcagtt
    37441 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    37501 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctttcca gccacatgat
    37561 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    37621 gccaatcagc gtaatggaaa gcgagcacga tgaagcgggt gaactgctgg aagtaatcaa
    37681 acacactacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    37741 taacggcatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    37801 actgttccca cgcgcgctgg cgggtgagtg atctaaaagc tggatggcat tgcgccatcc
    37861 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacagcca
    37921 gcccaacccc agcgcgataa accacagcgg agtaaccagc agcgcctggc gagtgtcatc
    37981 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    38041 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    38101 caccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    38161 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    38221 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    38281 agcgaacgct ttcggcgcaa caccttcctg cgccagacca aacagcatac ggctggtaga
    38341 gaagacaccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    38401 ggcagccgca ggcaaaccta ccaacacaaa cagctcaacg aacgggcttt tctccgggac
    38461 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    38521 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    38581 cgcagctgta gtacctacca gctcaatccc cacgaaagcg aaaaccgcta tctggaatcc
    38641 ggcaaagaag ccacttaaac ctttcgggaa ccagccgccg tcattccaca aatgcgcgaa
    38701 tgacgcttcc acaccggtcg gtgactgaaa gtgcatcgcc accatgacca ggccgacgac
    38761 aatcagcgac acgatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    38821 cattttcacg gtggcaaggt tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    38881 ccagtcggag agatcgggga accagaactg agcataagcc gtgatcgcca ccacgtctgc
    38941 cataccggtt acaacccagc agaaccagta ggtccagccg gtgaaatatc ctgcccacgg
    39001 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    39061 ttcccccatt gcccgcatca cgaaaaagag cataaaacca atgatcatat aaacgaaaat
    39121 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    39181 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    39241 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    39301 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    39361 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    39421 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    39481 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    39541 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    39601 agaatttcca gcagttcgac ttcaaacacc agggtactga acggagggat ggatgcgcct
    39661 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    39721 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg aaattcagcg
    39781 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    39841 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    39901 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    39961 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgctcg
    40021 tggatttcac gcagcgcgcg atgcaccaca tcaaccggaa cagccggatg tttgccttcc
    40081 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    40141 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    40201 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    40261 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    40321 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    40381 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    40441 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    40501 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    40561 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    40621 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcaca
    40681 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    40741 ggaaggccag ccagaagagc agccgcaaac tacccaaacg caaccattcc agccagatag
    40801 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    40861 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    40921 gggtaagccg ctgagtaatt tgcaaaacgg gcaaatggtg aaaatccgcc agaacgccag
    40981 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    41041 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    41101 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctgggggga aaccatactg
    41161 atatgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccgcc aagcgcagca
    41221 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    41281 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgctgt
    41341 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccag
    41401 gggttagcaa tataataatc ccatgtaaaa tcaataattt tctcaatggc tttttgtggc
    41461 ggaagatctg agaaatcaag ggccttttct ttttgtcgaa tatcgttcca ggtatattcc
    41521 agtacttcaa taaataaatt ttctttatta ccaaagtgat gataaaccgt ctgtttacta
    41581 caccccgcag ccttaacaat attatcgacc ctggcccctt cataaccata ttcggcaaat
    41641 tcattaaccg cgcataacag cagcttttcc ttcaggctct gtacgttgct ggtatcaaga
    41701 tacataacgc ttccccacga cctcttcaag ataactatct taacccgatt aattagaagc
    41761 tccagtaaaa aggataaaaa cttgtacttt catcaagtta tcaaatgtcc tgtgatcact
    41821 atcgcatatc aaccaaccag atggttgatc tttcttgcgg cacgtcgcaa gctaattgtc
    41881 acttagaaaa caatattcaa aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    41941 cgtgattacc ggagccgctc gtggtattgg aaagggctgt gcgctggagc ttgctcgtgg
    42001 cggatttaac ctgctgatta acgatcttcc cgatgccgac agcgtagaaa aactgcatat
    42061 cacacaacaa gaatgtatag ctgaaggcgt ggaagtgatt tgcttccctg ccgatgtcgg
    42121 cgatctctcc ttgcatgaag agatgctcga cgcggcgcaa aaccagtggg gacgtctgga
    42181 ctgcctgctc aacaacgcag gcatttcagt gaaaaaacgc ggtgaccttc tcgacctcga
    42241 accagacagc ttcgaccaaa atattgccat taacacccgc gcgccgttct tcctcgctca
    42301 ggcattcagt aaacgtctgc tcgcacagcc gaaaccggaa gcagaactgc cgcaccgctc
    42361 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatatac
    42421 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    42481 acaaattggc gtttatgaag tgcgccccgg tctcatcaaa accgatatga ctattccggc
    42541 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    42601 tccggctgat attgcttcca ctgtccgcgc gatggcggaa ggtaaactga tttacacctg
    42661 tggtcaggcg gtcgccatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    42721 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcaacac tgggctataa
    42781 aacgccgcta cccgccatta ttgatgcctg cgccgcgcgt ggtattggcg cgattgctcc
    42841 ctggcgcagg gagttgcaag gtgaagatct ccagcaaatc gcccaccagc ttgccgccag
    42901 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    42961 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    43021 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc acaaaagatc tgtacgaagc
    43081 gcgggaacaa gtaaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    43141 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    43201 gttacgccag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    43261 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    43321 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttagtgccga ccactgatct
    43381 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    43441 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    43501 gcagaaagat attaacagca cgctggatat tagcgtcgat cgcatcgcgc attattgtta
    43561 aggaatgatc atgagtgacc aacccgttct gttcagcaga gtggcggctt cctgccgcct
    43621 gacattgaac cgtgaagata aatgtcatgc tattaacgaa gagatgatcg aatctctcga
    43681 ccattattta aatgaaattg aaaacgacac cacattacga ttagtcgaat taacggcaac
    43741 cggcgataaa ttcttttgtg cgggcggcga tattaaatcg tggtcggcct attcgccatt
    43801 agatatgggc agaaaatgga ttaaacgcgg taatgacgtt tttaatcgcc tgcgaaactt
    43861 accccaatta accgttgcga atctcaacgg tcataccatc ggcggtggta ttgaactggc
    43921 cttgtgctgc gatattcgta ttgcccgccc cggcgcgaaa ttctctaacc cggaagtgat
    43981 gctcggcatg gtgcctggct ggatgggcat tgaacgtgtg cttaatcagg ttggtccggt
    44041 ggtcggtcgc cagatgctga tgctgggtaa acgcctgacg gcgcaggaag cccaggcggc
    44101 gaatttaatt gatgaagtgg tagaaaaaga acaggtggaa agctggatgg cgaaccagtt
    44161 agcgcagctg gaaaaatgcg gtccggtggc gcttgcacat attaagcaac tgattctggc
    44221 actggaaaat aaacatgccg attacccgca ccagctactg gccggattaa tgtccgccac
    44281 tcaggattgc cagcaggcga cgcgcgcctt tgccgaaaaa agtagcgtaa gcttccataa
    44341 tcaataagga aattgtatgc gtaagataac aaccgccgag gcactggcgg cgcaaattca
    44401 ggacggtgcg actattgcta ttagcggtaa cggcggcggt atggtggaag cggaccatat
    44461 tctggctgct attgaagcgc gtttcctgca aaccggacac cctcgcgacc tgacgctaat
    44521 ccactcgctg ggtattggcg atcgcgactg caaaggcact aatcgttttg cgcacgcaga
    44581 gatgctcaaa cgcattattg ccgggcactt tacctggtcg cccaaaatgc aggcgctggt
    44641 aaaaaataac acgattgaag cctactgctt tcctggtggc gtgattcagg cactgctacg
    44701 ggagatcggc gccggacgtc cggggctttt tacccacgtt gggctgggat cgtttgttga
    44761 tccacgcaat ggcggcggta agtcgaatga atgcactacc gacgacctgg tagaactgat
    44821 tgaaatcgat ggtgaaacca aacttcgtta tcgccctttc aaagtggatt acgcgatttt
    44881 gcgtggcact tatgccgatc ctcgaggcaa cgtcagcctt gaagaagaag cgattgatat
    44941 ggatagctat tccatggcgc tggcagcaca caacagcggc ggcaaagtgt tcgtacaggt
    45001 acgcgatgtg ctggaagctg gcgcgattga accacgccgg gtcaaattac cgggcattct
    45061 ggttgatggc atcgttgaac accgcgaaca accgcaaacc tatcttggcg gttacgacct
    45121 gaccattagc ggtcaacatc gccgtctgag ttctaacgac gctattgaac tggttagtca
    45181 tccggtgcgc cgcctgattg cccgtcgggc agcacgcgaa ctggtggctg gcgcttcaac
    45241 caactttggc tttggtattc ctggcggtat tccaggcgta gcgctgcgcg aaggcgttcc
    45301 ttaccaaagt ttatggctga gcgtcgagca aggcgtacat aacggcatga tgcttgatga
    45361 tgcgtttttc ggctgcgccc gcaacgccga tgccattatt ccatcactcg atcaatttga
    45421 gttctacagt ggcggaggga tcgatatcac cttcctcggc atgggagaga tggatcagta
    45481 cggtaacgtc aacgtctccc atctcaatgg caatctcatt ggtcccggcg gttttctcga
    45541 aattgcgcaa aacgcccgta aagtggtgtt ctgcggcacg ttcgacgcca aaggtagcaa
    45601 gattgatgta acgccagatg gcttgcatat cacccagtca ggtcaaatcc ctaaactggt
    45661 tacccaggtg gaaaaaatca cttttagcgc cgcctacgca cagcaaagtg gtcaggaagt
    45721 gttgtatatc actgaacgcg cagtattcca gttaacggca gaaggcgttg aattaattga
    45781 aatcgcgccg ggtgtggaga ttgagcgcga cattctgccg tatatggctt tccgtccaat
    45841 tatcaagcac ccacgcctga tggaaagtag cctgtttacg ccgatggagg atgcatgagt
    45901 cagcttgatg acaccatcct tgatgcgctg acgcacgtta ctttcccaaa gggttttgca
    45961 caggcagagc ccgcatgggt tgtcacggta gacggtgttg attacccact ctggcaaaca
    46021 gatgccctgg tagtcggcag tggtgcagca ggattacgtg cggcagttga acttaaacgc
    46081 cgccagcaaa atgtgctgat cgctaccgcc gggttatata tggggacgtc ggcatgttct
    46141 ggttccgata aacagacact atttaccgcc gctaccgcgg gcaacggcga caacttcacc
    46201 aaactggcag aggcactggc gagcggtggg gcgatggatc acgacaccgc ttatgtcgaa
    46261 gcggtaggtt ctctgcacac tctaggcggg ttgcaatatc ttggtctgga attaccggaa
    46321 gatcgctatg gcgcgattct tcgttatcaa accgaccatg acgaagccgg gcgtgcaacc
    46381 tcgtgtgggc cgcgtacctc aaggttaatg gtgaaagtgc tgttggaaga agtacagcgc
    46441 ctcgccattc cagtgttgac cagtgcaaca gtgattaaac tgctgcatca gcgtgacgaa
    46501 aacggcgaag accgcgtggc aggggcaatc ctcgcgaccg gtcatcgcgc ccataaccca
    46561 tgggggctgg caattgtgac tgcgcccaat gtggtactgg caacgggcgg accgggcgag
    46621 ctttatcgcg acagtgttta tccacataaa tgcttcggct cgctggggct ggcgctggag
    46681 gaaggtctga cgctaaccaa tctgaccgaa agccagtttg gtataggcac gccgcgtagc
    46741 acgtttccgt ggaatttatc cggcacctat gtgcaggtga tcccgtatat ctattccgtg
    46801 gatgctgagg gtaacgagta taacttcctc gcggattact atcgcaccac ccaggagctg
    46861 gcttcaaaca ttttccgtaa aggctatcag tggccgttcc acgccactcg ggtgatggat
    46921 tttggctcca gcttgttaga tatggcagta gcgcaagagc agcaatcagg gcgtcaggta
    46981 tttatggatt tcaatcgcaa tcctgaagcg gtgccgggtg acctgccgtt ctcattagat
    47041 cgactggacg acgacgttcg cgcgtatctg gaaaataacg acgctctggc accatcgccc
    47101 atcgaacggc tgcaacgaat gaatccgctg tctatctcgc tgtataagat gcacggttac
    47161 gatctcacca cacagccttt gcagtttgcc atgaataatc agcatatgaa tggcggcatt
    47221 gaagtggata tctggggaca aacctccctg cccggctgtt tcgccgtggg ggaagttgct
    47281 ggcacacacg gcgtcactcg ccctggtggt gcagcattga atgccgggca ggtttttgct
    47341 gttcgtctgg cacgttttat tggttgcacg caaaagcgga atattgatgg agatatagca
    47401 cagctggtag ctcctacact ggcttctata agagagatca ttactcaggc gcacgataac
    47461 gggaccggga tgccgttgtc ggttgtgaga gaaaaaattc aggcacgaat gtctgaccat
    47521 gcgggattta tttgccatgc cgataaagtc cgacgcgcca ctcgcgatgc cctgctattg
    47581 agcgaatttg tccaacggca tggattggct atcaaacatg tgggtgaagt tgccgagctg
    47641 tttatgtggc ggcatatggc actgacttct gccgccgtat taacgcaatt aacgcattat
    47701 attgacgcag gtggtggtag tcggggagca cgtattattc tcgacaggga tggaaattct
    47761 attccgcaaa cacgaaatgg tttttgtgat gcctggcgct ttcgttcaga gcgtactgag
    47821 gataaaaaag ataaactact tattcattat tgcaacggta tctttcacgt cagagaaaca
    47881 cctgttcgtg agtttcctat tatccgaggt atttggtttg aaaaaaactg gcctggtttt
    47941 cttaatggga caatttacca gccacaggat gaatgatatt gcaaaggtta atcactatgc
    48001 gaaatataat tagatctatt aatgctctac ttgcagcatt gaacattacc attttggcga
    48061 tcatcgtggc atgtgtgacg tggcaggtgg ctgcacgatt tatatttact agtcccagta
    48121 tttttacaga tgaactcagc cgtttgctct ttatttgtct tggacttttt ggtggggcat
    48181 acaccgcagg gcaaaaccgg catctggcga ttgacctgct acctatgatg ctgaaaggaa
    48241 aagcccgacg tcatttattt ttgtgtattc agattatcgt tattatcttc gcaaccatca
    48301 ttatggtata tggtggtggg ctattaacga tggatacatt cgattcaggt cagacctcgc
    48361 ccgctttagg ctggcaaatg ggatatatct atatgagcat cccgattagt ggtgtactta
    48421 ttattatata caccatagat atggttttaa ccgaacttaa acaaccactc taaagataac
    48481 aaacaacaac caaccatagt aggaatggag aaattatgga ttactggtta ccgattattg
    48541 tgttatttgg tgccttcttt tttatgttgg cattaggtgt acctattgtt tatgccattg
    48601 gtttatcgac actggcatca atttccacac aactcgattt caactcagca ttgagtgtgg
    48661 tttcccaaaa gttagcctcc gggttagata gttttacgtt gttggcaatc ccatttttta
    48721 tcttgtctgg caacattatg aaccatggag gaatagcgcg acgattaatc aactttgccc
    48781 gtattctcgg cggtcgtctg ccaggctcac ttgcccactg taatattctc gccaatatgc
    48841 tgttcggggc aatttctggt tcagccgtgg cttcggcggc tgcgatgggc ggcgtcatgc
    48901 atccgcaaca ggttaaagag ggttacgacc cggcattcag tactgcggtt aacgttgcct
    48961 ctgccccgac gggtttgctt attccaccaa gtaatacgtt aatcgtctat tcactggtca
    49021 gtggtggtac ttctattgcc gcactgttcc tggcgggtta tgttccggga attttgctgg
    49081 gtctggcact gatggtaata gccggtatta tcgcggtacg tcgcggttat ccaaagccag
    49141 aacgcccgac tttacgccag gctggcgttg cgatatggat ggctatcccc agtattttcc
    49201 tgattatttt aattatgggg ggtgtattaa gtggtatttt taccccgaca gaggcctccg
    49261 caattgcagt aatttatacc cttttccttg cactggttct ttatcgtgaa atctcagtta
    49321 aagatttacc taaaatattt ctggaatccg ttattaccac tgcaattgtc ttgttattaa
    49381 ttggttcttc gatgggtatg tcatgggcaa tgtcaaacgc tgacgttccc ttcctgattc
    49441 tcgatttgct taacactatt tctgacaacc caataataat cctgcttatt atcaatatta
    49501 tcctgttgat tatcggcact tttatggata tgacaccggc ggtactgatt tttacgccga
    49561 tatttttacc ggttgtcacc gaactgggca tggacccgat tcactttggt attgtcatgg
    49621 tactgaacat gtgtatcggt atttgtactc cgcctgtagg cagtgtgtta tttgtcggtt
    49681 gtagtgtatc taaattaccg attaataaaa ttatcaaacc tatgctgcca ttctatgccg
    49741 tgatggtatt ggtgctggca atggtgacct atattccgca aatcagtatg gcattgccaa
    49801 gagcactggg ctactgatgc gcattcactg gcggcacctg ccgccatatt tattagaaaa
    49861 ggaacatcta tgaaaataaa agtatccgct ggcattatcg gtgctgttct tatgttatcc
    49921 gcaagccagt cctgggcagt gacattaaaa ctgagtcata atcaggataa gtctcatcct
    49981 gttcataaag cgatggagtt ctttgcgaaa aagagcaaag agtactctaa cggtgatatt
    50041 actattcgta tttatccaaa tggaacattg ggtactcaac gagaaacaat ggagctgatt
    50101 cgttctggcg ctattcccct ggtaaaaacc aatgcggcag aaatggaagc atttgaaaat
    50161 ttctataaat tatttagcct gccttatttg ttccgcgatc gtgatcatta ttatcaggtc
    50221 atgcagggcg atatcgggag aaaaatcctc gactcaacga aaagcaaagg ttatttcggg
    50281 ctgacttttt atgatggagg cgcccgcagt ttctatggca ataaaccagt actgaaacca
    50341 gacgatctga aaggcatgaa agtccgtgtc cagccaagcc ctggcgcagt tgaaatgatc
    50401 aaagtcatgg gcggtaaccc gacgccactg gattacggcg agttgtatac agccttacag
    50461 cagggtgtgg tcgatatggc agaaaacagc gtgatggcgc tgaccaccat gcgtcacggt
    50521 gaagtggcaa aatccttcag ccttgacgaa cacactatgg ttcccgatgt ggttctgatg
    50581 agcaatgctg cgtttgataa acttagcccg gaaaatcagg cagttatatt aaaagcagct
    50641 aaagaatcaa tgagctatat gaaagacttg tggagcgagg aagagaaaca agaatttgcg
    50701 aaactggata aaatgggcgt gaaagtctac caggtagata aagctccgtt tatcgagaaa
    50761 gtacagccaa tgtacgcaaa cttcgctaag gacaacccag cccttgcccc aatgctggct
    50821 gatattcagg cagctaagta attatcttcg tagggctgct tattgtggcc cttatcgata
    50881 tctgcaagtg ctcgctacaa aagcaaaacg ccgaccaatg gtcggcgttt ttacgtctcg
    50941 ttgaataacg aattattcag ctactacgtt tacgatcact ttcgcgaata cttcgctgtg
    51001 aacctggaag ctcacttcgt gttcgccagt ggtacgcaga acgccgttcg gcagacgaac
    51061 ttcgctctta gccacttcaa cgccagctgc agttacagcg tcagcgatgt cgcgagtacc
    51121 gatggaaccg aacagtttac cttcgtcgcc agctttagac gcgatggtaa cagtttccag
    51181 tgcattgatt ttctcagcgc gagcattagc agctgccaga acttcagcca gtttagcttc
    51241 cagttcagcg cgacgtgctt cgaagaattc aatgtttttc ttggtagctg gaacagcttt
    51301 accctgcggt accaggaagt tacgagcata gcccgcttta acgtttacct gatcacccag
    51361 gctacccagg tttgctactt tatcaagcag aataacttgc attaccttat cctctcaaag
    51421 tcgtattaat ggaccgtgac cgattactga tggcgatcag tgtacggcag cagggacagg
    51481 tagcgagcgc gtttgatagc gcgagccagc tgacgctggt attttgcacg ggtaccggtg
    51541 atacggcttg ggacaatctt accgctttcg gtgatgtagt ttttcagcgt agcgatatct
    51601 ttatagtcga tctcttgaac gccttccgcg gtgaaacggc agaacttgcg acgacggaaa
    51661 taacgtgcca tatggctagt ctccagaatc tatcaattca atctgctcgg catgcaaaac
    51721 cattttgctc agtccgttct ttgccttgtg gcatgaaatg aacccctgaa cggttatgcg
    51781 actgccgacc gttatactgt gagtaatggc ctggttttcg tgtccgctaa caataacggg
    51841 catttgacac catgcctgcc ggtgaaagcc ggcttcctcc tgcacagaac gatgctcaag
    51901 cacgaactgg cagtgaggaa ttcctgatgg actgaccttt cgaaggggag tcctgcacac
    51961 ggtgccggac aacaccagac ggttggtcat cagaaattac tcttcagaat ccccagcttc
    52021 agcatcatca gcggtttcgt ttgcgaaatc atcgcgacgc tcacggcgct cgtctttcgc
    52081 tttaaccatc ggagatgctt cggtaacagc gtgcttggta cgcataacca tgctgcggat
    52141 aacggcatcg ttgaagcgga aggtagtttc cagctcatcg atcacttcct gcggagcttc
    52201 aacgttcatc agaacgtagt gtgctttgtg cagtttgttg atcgggtaag ccagctgacg
    52261 gcggccccag tcttccagac ggtggatctt gccttctgca ccagtgatgg cagcagtgta
    52321 gcgctcgatc atgcccggaa cctgttcgct ctggtcagga tggaccataa aaacgatttc
    52381 gtaatgacgc atcgaattgc tccttacgga ttattcagcc tcctgtctgg gtcagccgaa
    52441 tcccggggag gcaaggaacg tgttaaaggt cggctgaaaa atgacgcgtt atagtacttg
    52501 tatgccccgc taaactcaag ggcagagccg aaataaatct cccctttcgc aattaacctc
    52561 taaaccgctg atttggagtt catcacactt catcatggtg tttttttgaa caactgcatc
    52621 agaaatggtc accatcaact tctttatcat tgattaaaat taaatcagaa gatcatcaca
    52681 gaggagagca cacaaagtca gccgtcgagg aggtatctat gttcagtcga gttttagccc
    52741 ttctggctgt gcttttgcta agtgcaaata catgggcagc cattgaaatc aataaccacc
    52801 aggccagaaa tatggacgat atgcaaagct taggcgtgat ttatatcaat cataatttcg
    52861 ccactgaaag tgaagcacgt caggcattaa atgaagaaac agatgcgcag ggcgcaacgt
    52921 actaccacgt aattctgatg cgggaaccgg ggagtaacgg caatatgcac gccagcgcgg
    52981 atatttatcg ctagcaccag gtataaccaa cgaaacattg ccatagtttg ctttgccccc
    53041 ttcgcagggg gctttttttg aaaaacggct gcggaattag accagttatc tcccgaagaa
    53101 ggaaatttcc gcagcgcgtg ttttacttct gcccgtaata agcgttagga ccatgcttac
    53161 gcatgaagtg tttattcatc aggaagctgt cgatgtgatt gagttgtgga ttaatactgc
    53221 gcgcaatcca cgccattttc gccacttctt ccatcactac cgcgttatgc accgcgtcgt
    53281 gggcatcttt cccccaggcg aacggtccgt gctgatacac cacaattccc ggcgtatgca
    53341 gcggctcggc gttgcccagc gtttcgataa tcactttgcc agtgttcagt tcatactcac
    53401 cctgcacctc ttcttcgctt aatccacgcg tacacgggat gtcgccaaag aagtagtcgg
    53461 catgcgtagt gcctaacgcc gggatcgcca gccccgcctg cgcccatgcg gtagcatgag
    53521 tggagtgggt atggacaatg ccgccaagcg acggataacg acggtagagt tcaagatgcg
    53581 tcgcggtgtc ggaagacgga cgatattccc cttccaccac cttaccgctc atatcgacca
    53641 ccaccatatc gtctgctttc atggtttcgt aggcgacgcc gctgggcttg atcaccacca
    53701 gcccgcgttc gcggtcgata gcgctgacgt tgccccaggt aaaggtcacc agcccgtagc
    53761 gcggcagatc catgttggct tcaaatacct gctgttttag cttttgcatt atgccgcctc
    53821 caccatgccc gctttcgcca tgcgcgcttt cacccaatca cgcgctttcg ccacttctgc
    53881 cgccgggtct tccgccgttt cgctccacat ctcaatcagg tacggcccgc aatagccact
    53941 ctgtttgagc gtttcgaaac aacgttcgaa atccactaca ccttcgccaa acggcacgtt
    54001 tttgaagacg ccaggtttgg tgtctttcac atgtaccgcg acgatatgcc cgattccggc
    54061 ctgcaactcc atctgcacat cgttgtccca cgccgacagg ttgccgatat ccgggtagag
    54121 ctggaaccac gggttgttga gatagtgcgc gtaacccagc gccttgctga tggagttcat
    54181 caacggataa tccatgatct ccatcgccag cgtcacctgc gcgcggctcg ccatctcaac
    54241 gctctctttc aggccgtcac ggaaacgacg acgcgtttcg ttattggctt cctgatagta
    54301 aacgtcatag cccgccagct ggatcacgcg aatgccgacg tcctgggcga actggatagc
    54361 tttacgcata atctccagcc cctgcgcccg caccgcgtca tcttcactgc ccagcgggaa
    54421 acgacgatga gcagaaaggc acatggacgg cacgcgcacg ccggtttcaa caatcgcatt
    54481 caccagcgcc agacgctgct cgcggctcca gtcgaggcgc gacaggcgct cgtccgtttc
    54541 atctaccgac atctcgacaa aatcgaagcc caacgttttt gccagttgca ggcgttccag
    54601 ccagcactcc ccggcgggga gcgctttttc atagatgcca agcgggattt gtttggacaa
    54661 catatccgct ccttagcccc acagttcggc gatggaacgt ttgaactgac gcgcggcttc
    54721 aaccggagaa gcggcatcac ggatgctgcg accggcgata aacacatgaa ttggaatgcc
    54781 tttgaacagc ggcagatctt ccagcgccag gccaccggta acggtgactt tgaagcccat
    54841 atcggaaaga cgtttgatcg cagtgatatc ggcttcaccc cacgccacgc cagcggcctg
    54901 cgcgtcacgg ctgcggtgat aaaccacctg ctgaatacct gcgtcgcgcc actgttgcgc
    54961 ctgttcccag gtccagtaac cggtcagttc gatctgcacg tcgccgttaa actcttttgc
    55021 tacgtccagc gcacctttgg cggtgttgat atccgcacaa caaatcacgg tcacccagtc
    55081 agcgttggct tcgaagcaca tacgagaaag aattttgccc gcatcggcaa ttttggcgtc
    55141 tgccagcacg attttgtgcg ggtagagcgc tttcagatcg cgaaccgcac gcacgccttc
    55201 gcccacgcac agaatggtac ccacttcgat aatgtcgact tcttcggcaa tcaggcgagt
    55261 ggtttcgtag gcgctatcca tagtctggtt gtccagcgcg acttgcaaca tcggtaatga
    55321 catcttcaat tccttcttaa gctgccgcgt tggtgcggtc aatgagatcc agtacttcct
    55381 gctcggtacg gcaggcacgt aaacggtcga aattttcctc atcttcaaac aggttgacga
    55441 tctgcatgat gcccacttcc tggtgcgtgt tggcatcgac cgccgccatg gtgatgagga
    55501 tatccaccgg gtcgttatct tcgtggttga actccagcgg ctttttcagc gtcaccagcg
    55561 caaaaccggt tttcttaaca ccctcttccg ggcgaccgtg cggcattgcc aggccaggtg
    55621 cgagcacaaa gtacggaccg tgctgttcaa ccgcatccag aatcgcctgg tagtaacgcg
    55681 gctcgacaac atccgccgcc accagcaggt caacgccgat ttttactgcg tcctgccatg
    55741 tctcagcttc agcctgcagg cggatggatt tattttccgc cagcgaatca cgtaatttca
    55801 tggcgcgtcc ttacttcaca tcctgcggga aatgctcttt gatcacttcc agcagtttcg
    55861 ggccaaaatc ggcaggagag agcatgttgc gcacgccaac cacgtatttg ttgccggtca
    55921 cggagatctc gcccgcgatg tgcgtggaag cgatgataat atccgcgttg ctcagctcac
    55981 ttttgtactc gccaaccgcg cagctgttta ccgtgtggtc aatctttgat tgggttaaaa
    56041 actggtccac tttcattttc atgatcatgg aactaccttg tccgttgcca cacacagcca
    56101 gaatacgtac ggtcataatc aaaactcctt attaagcaga ctgttctgcc agttgttttt
    56161 ctgcatcttc ttcagcgcgc agtgcgcggc cagcgaagaa catataagcc agtgcaatta
    56221 caatgatgac ggccataaag gcgataccga tggagaagaa gccctgcatc atcggcggtg
    56281 ccagaatcga ccagtccgcc atgcccatcc aggcgctcat accggtgagt ttcaccgccc
    56341 agacgcagcc aaagatttca atcatcccca tcaccagaca tatcttcagc gccgcacgcc
    56401 agccgccgaa gtggttagcg aacacgccga tggtggcgtt ggagaagaac atcgggataa
    56461 agccgggaat aatcaggatc gaggaaccgc aggcgaccag gatgccaacc gcaatcagct
    56521 gaccgatggt gccccacata aagccccaaa ccacggcgtt cggcgcgaag ctatagatag
    56581 ccgcacagtc aatcgccaga accgcaccag ggatcaggcg ctgggagata ccgttaaatg
    56641 cttcagagag ttccgccaca aacatgcgca cgccctgagt gatgatgaag atcgccaccg
    56701 cgaaggagaa accagtttgc aggatgtaca ccgtccagtt cactttgcct gccatcgcct
    56761 gcacggtgtc gataccgaag gagagcagaa tggcaccaaa gaagatggtc atcacaatcg
    56821 ccgtggagac gatgttgtcg tggaaaatat tcagccagcc tggcagtttg aggtcttcaa
    56881 cgctttcctc ttttttgccg aggaacggcg caactttata ggcaatccat gatgcaaact
    56941 gttgctggtg accgatggag aaaccgcagc cgtccgtcac ttcctgagtc ggcttgtaca
    57001 ttatgttgga ggtaatgccc cagtagagcg aaaccagaat cgccgtgcag ataatggtcg
    57061 tccacatgga gtagccgaaa ataaacaacg taacggcaat cagccccgcc tgctggaaca
    57121 tgatatgacc agtcagcatg atggtacgaa taccggtaat gcgacgcagc agcacgtaac
    57181 agatgttcaa cgccagcgct aacaacaccg cgtaacctac ccagttatag gcatcgccca
    57241 tgcggtcgat ggttgccatc atcgacgcgt aagtatcgga aattgcgccg ttaatgccgt
    57301 agacttcgga cattttcgcc accaccggtt tgaaggtgct ggtgaggatg ccggaccctg
    57361 cctgcaacaa catgaaacca attatggttt taatcgtgcc tttaataata acgctgacac
    57421 ttttgcgcag taggatgtag cccagacagg tcacaatacc cagcaacaac ggggcattgg
    57481 tcatgacctg gttaaaaaac acggtaaaga tgttgtagag gatctccata acgatctcct
    57541 gaagaagagg taaccgggta ttccacacac ccggaatgtt gtgcactcac tctaattttc
    57601 aaaagtaatc acaacaagat tatttatgat taaatgtgac gcgacccgca aataattcca
    57661 catggataga atgggattca cacaacgaaa caactattca tttaaattca tttagttaat
    57721 tgaattacac attccattaa atctttccac actcaaaatc aacaaccact tccctggcaa
    57781 aaagtcaaaa aatggtcatt gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa
    57841 tcacatattg attcgaattg aactatcatg aaggtaaatg gcgatgagta aagtgaaaag
    57901 tatcacccga gaatcctgga tcctgagcac tttcccggag tggggtagct ggctgaatga
    57961 agaaattgaa caagagcagg tcgcctctgg cacatttgcg atgtggtggc taggctgcac
    58021 cgggatctgg ttgaaatcag aaggtggcac caacgtttgc gttgatttct ggtgcggcac
    58081 tggcaaacaa agtcacggta acccgttaat gaaacagggt caccagatgc agcgcatggc
    58141 tggcgtgaaa aaactgcagc caaacctacg caccaccccg tttgttcttg atccgtttgc
    58201 gattcgccag atcgacgcgg tactggcgac tcacgatcac aacgatcata tcgacgttaa
    58261 cgtcgctgct gccgtgatgc agaactgtgc tgatgacgta ccattcattg gaccgaaaac
    58321 ctgcgttgat ttgtggattg gctggggcgt accgaaagag cgttgcatcg tggtcaaacc
    58381 gggcgatgtt gtaaaagtga aagacattga aattcatgcg cttgacgctt tcgaccgtac
    58441 tgcactgatc accctgcctg ccgatcaaaa agcggctggc gtactgccgg acggcatgga
    58501 cgatcgcgcg gtgaactatc tgttcaaaac gcctggcggc tccctgtatc acagcggcga
    58561 ctcccactac tctaactatt atgcgaagca cggtaacgaa catcagatcg atgtggcgtt
    58621 aggatcgtat ggtgagaacc cgcgcggtat taccgacaaa atgaccagcg ccgatatgct
    58681 gcgtatgggt gaagccctga atgcgaaagt ggttatcccg ttccaccacg acatctggtc
    58741 aaacttccag gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa aagatcgtct
    58801 gaagtatggc ttcaagccgt ttatctggca ggttggcggc aaatttacct ggccgctgga
    58861 taaagacaac ttcgagtacc actatccgcg cggtttcgat gattgcttca ctattgaacc
    58921 ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat accggatagc gtggagaagt
    58981 tcgcgctatc cggcgtagta tttcctggct atttcaaata tcatctaaaa aaatcaaatt
    59041 ttatcggaat agctcatgac tgaagcacaa agacatcaaa tcctcctgga aatgctcgca
    59101 caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc gcctgccact
    59161 gcgcgacgcg atatcaataa acttgacgaa agcggcaaac tgaaaaaggt gcgcaatggc
    59221 gcagaagcta ttacccagca gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag
    59281 aatcacgatg aaaaagtacg tatcgctaaa gcagcctcgc agctggttaa tccgggcgaa
    59341 agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag
    59401 ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt acctgatcga tcaagaacat
    59461 gacagcgtga tcattatggg tggacagtac aacaaaagtc agtccatcac tttaagcccg
    59521 cagggcagcg aaaacagtct ctatgccggg cactggatgt ttaccagcgg aaaagggctg
    59581 accgcagaag ggttgtataa aaccgatatg ctgacagcaa tggcagagca gaagatgctg
    59641 agcgtggtag ggaaactggt ggtactggtt gatagcagta agattggcga acgcgcggga
    59701 atgcttttta gccgtgccga tcaaatcgat atgctgatca ccggcaaaaa tgctaacccg
    59761 gaaatcctgc aacaactgga agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg
    59821 cggaaaaatg tcacggcagc atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc
    59881 tgccatgaac aggttagcag tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt
    59941 agtgattcgt cggcaggcac aacgtcatcg tcgaggccat gccacagcag tagaggtctg
    60001 tccccaagtt gttccaggtg gtttgtcgct tcccactctg ccagcggcgc gataatgtta
    60061 ttgaattcat tctgctgtgc tgccgtttca ggtatcagcg gtggaaacag tgaacgggcg
    60121 agcgatgtaa aatagcccga tcccatcatg ctggcggtac atttcaccgt ggggtggcga
    60181 gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc caccgactgc cagacggtca
    60241 tcaagcagcc agttttcttc ggctattgcc gcacgtaaag tagtgaattc ctgcatactt
    60301 tgtagcaaga tttgccagaa ttgatttaac cgccgcgctt cgtcgctact aaaacggcta
    60361 ccgtgatcgg gcgcatccgg catgatcacc cgcaaaccag cctgcgccag cgcaacggca
    60421 aaatagctat acaccaggct ggatgaagta aagccgtgat aaaaaattac gcacggtaac
    60481 ggggtatctt tttgcccgac aggataagca tgaagaacgg gaatatctgc cagctcgcgt
    60541 gattctattt caatcatctg tctctccttt ttgtctggta tgacatgaag aaatccttag
    60601 cacaagtttc tgctggcggt attagttcat aatgttgaga tgtgggttac gctttcgtca
    60661 cgttttcatt cgaaaatcgc gtaccaggta acaattcggg aacattcccc caaagttaaa
    60721 ttaataaggc actacactat ggttagcagg aaacgtaata gcgttattta ccggtttgcc
    60781 agtttattat tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac gccacagcca
    60841 gcaccgccag taacgaacca tccgcaagag attcgccgcg accagacgca agggttacag
    60901 cgaataggta gcgtaagcac gatggttcgg ggttctccgg atgacgcatt agcagaaatc
    60961 agagcaaaag ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt ggacgaaacc
    61021 atcgtgacag gacagtggta ttcacaagcc attttgtacc gtaaataaat cagacaactt
    61081 acatagtcag ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac aataaattac
    61141 cggctggaag cctgggcgtt tattaccgcg atggaatgcc ctgcaacatg tggggaaacg
    61201 aaaaatggag ctgacgatga aacaattact tgcctcaccc tcgctgcaat tagtgactta
    61261 tcctgcgagc gccacggcac agtctgcaga attcgccagt gctgactgcg tgacgggcct
    61321 gaatgaaata ggtcagatct cagttagcaa tatttcaggg gatccgcagg acgtggaacg
    61381 tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta ttaccatgta
    61441 cgaagaccag cagcctgata actggcgcgt acaggcgata ctgtatgcat gagtgtcatc
    61501 tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt ttccggccta caattttcac
    61561 atatttcatt gagttaatcg aaaccagcgt cgcatcagtc gtatacgctt acacacacac
    61621 tccccccgtc gcccgcagca ataaatcatt ctggatctgc tctgacaacc gtacgccgcc
    61681 gcgcgtatct aacatcacct gacaccacgc ctgtgccatt ggcggagagg catatttcaa
    61741 catttgcgca ccgcagccca gcaggaatag ctgatgagta atctctcgcc ccaattcttc
    61801 agctggcttg cgcagctgct gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg
    61861 ccctttcact tccacaaacg cttccgacaa taagtcgtat acgcccgctt gcttattgag
    61921 aacgcgcagc acatccaggc acataatatt gccggaacct tcccaaatac tgtttaccgg
    61981 catctcccgg taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac
    62041 ctccatcgct tcggccacaa acggcatacc tcttttgcag atcacaaatt ttgccgcagg
    62101 cgtaaataaa cgcgcccaca gggcttcttt ggcatcggca cgccggtccc acgctcgcgc
    62161 aagacgaaac agcaacgccg tttgcccttc aagctgaagt gccatgcgac ttaagacatg
    62221 acgcataagg ggctgttgga tcaatggatt accaaaaaca tggcgttgat gtgcatgata
    62281 aatcgccagc gaaaatgcac ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg
    62341 cgtcatccca cccattttca ggatcagacg aattccttcc ccttcctgcc ccaacaacca
    62401 gccaatggca tcctgaaact ccacttcgca gctggcgtta gagcgattac ccagcttatc
    62461 tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa
    62521 aaagcaggac aaaccgcccg tagtctgcgc cagcaccaga tgcgcatcgc tttgcggcac
    62581 cgagaaaaac catttatgcc ccaccagccg ataagagcca tcttccaggc gctctgcgcg
    62641 ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa
    62701 caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc tcagcagcgg
    62761 cgtggtccag tcctgaaacg gcgcgggtaa catctgtaac agcaatggcg tggcggcaaa
    62821 ggtcatggtt atcggacata acgaccctgc ctcaacctgt gcgtgcaaca taaaacgcgc
    62881 cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc caggcaagat tgtgcacccg
    62941 attggtacat agcgcctgca tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag
    63001 acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga ttcacattcg ccagccgccc
    63061 cagttcaagg gattcagccg ttcctaactg ctgaccaata ctggctagaa aatcgctatc
    63121 ccagccagca ccttcacgcg ttaccgcttc gcagagcgcg ccatcagaca ggtataagtt
    63181 gctgttattt aatggtatag gttgattaaa aacggtgtga gtttgccagt gcactgtgtc
    63241 tccctccatc aatggcagtc accattagta tggtcactgc catggattca tgacagaaag
    63301 agattgctaa aacattctta tccagcaatg taatgcggaa cgaaacgtga ggtgtctttg
    63361 gtgatcagtg tgttatcttc acggatcccc attccgcacg cttcatcatc gacaatccag
    63421 ctaccgatga gtgtgtagct atcgccaaat cgcggcagag gttgaaacgc ctggtagatc
    63481 atcggttcat cggcgtaatc accatcagcg tggtcaacga cgttattctg accgtcgaaa
    63541 atggtgacgt ttccgccttc gcgcgagtag atcggtttac gcacatagct ttcgccagcg
    63601 gcaatctgcg atttttcacc ctcaaaccag gatgcaagaa ggttaggatg accagggaag
    63661 aagcgccaaa gcagcggcat tagcccttta ttactcaaga tacttttcca taacggctcc
    63721 acccattgct cgcgacgctt gcacagcagc ggaccgttat cgtcacgcat catccactcc
    63781 agcggataca gcttaaatgc acgctggatg acattatcat caagatcggt cagtacaccg
    63841 ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg actcctgccc tgcctgctgg
    63901 gcgcagtcct gcaagtacag cacggtagtc ctgtcttcat cggtgtcctg acagcagcag
    63961 aaataaaacg gctcccggct ataaagctcg ctaaagcgtg aaatcaggcg ttcctgaatg
    64021 gcattgtact gatcggcatc acgcggaata atgccgctgc gccgggcatc ctccagccac
    64081 aaccactgga aataagccga ctcatacaac gaggtcggcg tatcggcgtt gtactccagc
    64141 agcttcaccg gcgcattgcc acaccaggca aaatccatcc ggccatacag cgaaggatcg
    64201 cgtgcgcgcc agctttcggc gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc
    64261 aggatctctt cgtctttaac cgcgcgatcc accacctcaa ggcacatctg atgcagttcc
    64321 gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag taaaacggta agcccgactc
    64381 tcgtcccaat agatttcatt gtcgatgata tgaaagtcga aaccgttgtt agcggcgatc
    64441 tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct ccccaatgcc
    64501 cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac ggttgacgct tttttggtgg
    64561 taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag gagtaatcac
    64621 cggaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg ttatagacga
    64681 acggctcatc gcgatcttta cgaataactc ggcttaacaa aaatccagaa acgaccggga
    64741 tccaactctg ttcaacattg tcgtagtagc aattttcgta cttagactga caattctgct
    64801 gagtcatatt cttcggaaca tcggcataaa atgccgcttt ggcgttgttc cagccacgcg
    64861 cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc
    64921 cgtcgccgtc gttatcgaca tcgccacttt cactccatcc tttgactatt aaaaaaacgg
    64981 caccgcccat tagcaccaac gttaagtatt tttgtgcgta gcgattgcgg cgcggctcaa
    65041 aaggattatg cggtcttcca atgcgactga tcgctccgtg accaatttta ctgttatttc
    65101 ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct gcgttaaaaa
    65161 taccaccagc cagtgcggcg gttcccataa acattcctgc tgcggtattg tgattaataa
    65221 ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt aactgaatca
    65281 ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg attgccgcac
    65341 tggataaggg gatcacgtaa cccaacaatg taccgctgaa tgccagtgac gccgcggtat
    65401 tattgttttt gattaattgc cattcgttgt gcggtgtaat tttggagtag ataaacagaa
    65461 aaataatcac catagccacg ccaataaaaa aataggcact aaaggcaaga agtgaatcca
    65521 gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg tcagcgggat
    65581 atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt taaaagattc
    65641 ctcaccattt aacagcaaat attcgtaagt atcttcggtt acttggcgtt ggtagcccat
    65701 ggtaaaatta tggacctccc atttggcatg gttttgattt tctacttttt ccagcatgta
    65761 gacgggttcg atatttcccg gttcttcgct gttaaaaaat cgctgccagc gtttttcctg
    65821 ccagtttaag atcattgccc ccatcacttt ggcgttgatc gcctcgcgcc agtgactctc
    65881 tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa tgtcatcaat
    65941 atcttcgccg ccggtggtat tgatttgcag aaattcatcg cctgaagtgt agtagcggaa
    66001 aatctgacta ccgccgccca gatcgatgtg gctgacagcg gctaccgtaa attcctcacc
    66061 cggcagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt cgagcgtaaa
    66121 gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat tatccttgcc
    66181 aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc agccttttag
    66241 cgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg
    66301 gcgctacttt tattgctacc gccaattccg gcttccgcca gcttttcatc aagatcgcga
    66361 ccgtctgcga ctttttccaa ctgtgcagca gcatccaggc gagcctgacg ttcggcctga
    66421 cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg
    66481 gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc
    66541 atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt ttccacgcca
    66601 tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag attctcaagg
    66661 cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt gcttaacgct
    66721 tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag cttatcgtga
    66781 cttaatttca cccgcgccag cagatcaacg cgagattttc cggctttatc gagttcagct
    66841 ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc aatggattct
    66901 tccgcctggg agataaacga tttccccagc gtaaataaac tttttaaaat tcccataaat
    66961 acccgtcctt gtaattagtg tgaatattct tctgtaattt cagccagatc caatgcgtta
    67021 tctacctgcg aggttatctc aagcaggata tcttcaagag aagatttaag cgataacgcc
    67081 ccgaaaacaa tgtaatactc ttcctgttgc acgctggaga ttcctaccga tgacaacggc
    67141 atcatcttct gatttcttaa taagaaggta ttaaattcat ctggattgct gatactactt
    67201 accggacaaa taaaggtttc gataatcatt tggcgggaag taaaaagaat attgatttgc
    67261 aaatcgccat aatcattcat tttaataatt aatgcgctct cgctatttgt cacatcaata
    67321 ttttgttcag gtacagtttg cagcgccgtc gccagcgcc
//
LOCUS       DAZGCS010000012        65340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGCS010000012 DAZGCS010000000
VERSION     DAZGCS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.012"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..494)
                     /gene="folK"
                     /locus_tag="ACHK01_000822"
     CDS             complement(15..494)
                     /gene="folK"
                     /locus_tag="ACHK01_000822"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO8095818.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(491..1888)
                     /gene="pcnB"
                     /locus_tag="ACHK01_000823"
     CDS             complement(491..1888)
                     /gene="pcnB"
                     /locus_tag="ACHK01_000823"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO8095819.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1948..2844)
                     /gene="gluQRS"
                     /locus_tag="ACHK01_000824"
     CDS             complement(1948..2844)
                     /gene="gluQRS"
                     /locus_tag="ACHK01_000824"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO8095820.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2911..3366)
                     /gene="dksA"
                     /locus_tag="ACHK01_000825"
     CDS             complement(2911..3366)
                     /gene="dksA"
                     /locus_tag="ACHK01_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO8095821.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3544..4248)
                     /gene="sfsA"
                     /locus_tag="ACHK01_000826"
     CDS             complement(3544..4248)
                     /gene="sfsA"
                     /locus_tag="ACHK01_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO8095822.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4263..4793)
                     /gene="thpR"
                     /locus_tag="ACHK01_000827"
     CDS             complement(4263..4793)
                     /gene="thpR"
                     /locus_tag="ACHK01_000827"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO8095823.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4867..7296
                     /gene="hrpB"
                     /locus_tag="ACHK01_000828"
     CDS             4867..7296
                     /gene="hrpB"
                     /locus_tag="ACHK01_000828"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO8095824.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGFLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7492..10026
                     /gene="mrcB"
                     /locus_tag="ACHK01_000829"
     CDS             7492..10026
                     /gene="mrcB"
                     /locus_tag="ACHK01_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO8095825.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10246..12489
                     /gene="fhuA"
                     /locus_tag="ACHK01_000830"
     CDS             10246..12489
                     /gene="fhuA"
                     /locus_tag="ACHK01_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO8095826.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12540..13337
                     /gene="fhuC"
                     /locus_tag="ACHK01_000831"
     CDS             12540..13337
                     /gene="fhuC"
                     /locus_tag="ACHK01_000831"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO8095827.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIVQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13337..14227
                     /gene="fhuD"
                     /locus_tag="ACHK01_000832"
     CDS             13337..14227
                     /gene="fhuD"
                     /locus_tag="ACHK01_000832"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO8095828.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14224..16206
                     /gene="fhuB"
                     /locus_tag="ACHK01_000833"
     CDS             14224..16206
                     /gene="fhuB"
                     /locus_tag="ACHK01_000833"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO8095829.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16241..17521)
                     /gene="hemL"
                     /locus_tag="ACHK01_000834"
     CDS             complement(16241..17521)
                     /gene="hemL"
                     /locus_tag="ACHK01_000834"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO8095830.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17687..17734
                     /gene="yadX"
                     /locus_tag="ACHK01_000835"
     CDS             17687..17734
                     /gene="yadX"
                     /locus_tag="ACHK01_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO8095831.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17746..19167
                     /gene="clcA"
                     /locus_tag="ACHK01_000836"
     CDS             17746..19167
                     /gene="clcA"
                     /locus_tag="ACHK01_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO8095832.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19191..19256
                     /gene="yadW"
                     /locus_tag="ACHK01_000837"
     CDS             19191..19256
                     /gene="yadW"
                     /locus_tag="ACHK01_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO8095833.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19249..19593
                     /gene="erpA"
                     /locus_tag="ACHK01_000838"
     CDS             19249..19593
                     /gene="erpA"
                     /locus_tag="ACHK01_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO8095834.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19640..20263)
                     /gene="yadS"
                     /locus_tag="ACHK01_000839"
     CDS             complement(19640..20263)
                     /gene="yadS"
                     /locus_tag="ACHK01_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO8095835.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20301..21101)
                     /gene="btuF"
                     /locus_tag="ACHK01_000840"
     CDS             complement(20301..21101)
                     /gene="btuF"
                     /locus_tag="ACHK01_000840"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO8095836.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21094..21792)
                     /gene="mtnN"
                     /locus_tag="ACHK01_000841"
     CDS             complement(21094..21792)
                     /gene="mtnN"
                     /locus_tag="ACHK01_000841"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO8095837.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21876..23393
                     /gene="dgt"
                     /locus_tag="ACHK01_000842"
     CDS             21876..23393
                     /gene="dgt"
                     /locus_tag="ACHK01_000842"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO8095838.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23523..24947
                     /gene="degP"
                     /locus_tag="ACHK01_000843"
     CDS             23523..24947
                     /gene="degP"
                     /locus_tag="ACHK01_000843"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO8095839.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25102..26259
                     /gene="cdaR"
                     /locus_tag="ACHK01_000844"
     CDS             25102..26259
                     /gene="cdaR"
                     /locus_tag="ACHK01_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO8095840.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTELDLGNFDDRLLLYVALQLDEER"
     gene            complement(26348..26734)
                     /gene="yaeH"
                     /locus_tag="ACHK01_000845"
     CDS             complement(26348..26734)
                     /gene="yaeH"
                     /locus_tag="ACHK01_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO8095841.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27046..27870)
                     /gene="dapD"
                     /locus_tag="ACHK01_000846"
     CDS             complement(27046..27870)
                     /gene="dapD"
                     /locus_tag="ACHK01_000846"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO8095842.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27901..30573)
                     /gene="glnD"
                     /locus_tag="ACHK01_000847"
     CDS             complement(27901..30573)
                     /gene="glnD"
                     /locus_tag="ACHK01_000847"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO8095843.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30635..31429)
                     /gene="map"
                     /locus_tag="ACHK01_000848"
     CDS             complement(30635..31429)
                     /gene="map"
                     /locus_tag="ACHK01_000848"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO8095844.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31797..32522
                     /gene="rpsB"
                     /locus_tag="ACHK01_000849"
     CDS             31797..32522
                     /gene="rpsB"
                     /locus_tag="ACHK01_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO8095845.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32780..33631
                     /gene="tsf"
                     /locus_tag="ACHK01_000850"
     CDS             32780..33631
                     /gene="tsf"
                     /locus_tag="ACHK01_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO8095846.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33778..34503
                     /gene="pyrH"
                     /locus_tag="ACHK01_000851"
     CDS             33778..34503
                     /gene="pyrH"
                     /locus_tag="ACHK01_000851"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO8095847.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34795..35352
                     /gene="frr"
                     /locus_tag="ACHK01_000852"
     CDS             34795..35352
                     /gene="frr"
                     /locus_tag="ACHK01_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO8095848.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35444..36640
                     /gene="ispC"
                     /locus_tag="ACHK01_000853"
     CDS             35444..36640
                     /gene="ispC"
                     /locus_tag="ACHK01_000853"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO8095849.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36829..37587
                     /gene="ispU"
                     /locus_tag="ACHK01_000854"
                     /gene_synonym="uppS"
     CDS             36829..37587
                     /gene="ispU"
                     /locus_tag="ACHK01_000854"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO8095850.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37600..38457
                     /gene="cdsA"
                     /locus_tag="ACHK01_000855"
     CDS             37600..38457
                     /gene="cdsA"
                     /locus_tag="ACHK01_000855"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8095851.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38469..39821
                     /gene="rseP"
                     /locus_tag="ACHK01_000856"
     CDS             38469..39821
                     /gene="rseP"
                     /locus_tag="ACHK01_000856"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO8095852.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39851..42283
                     /gene="bamA"
                     /locus_tag="ACHK01_000857"
     CDS             39851..42283
                     /gene="bamA"
                     /locus_tag="ACHK01_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO8095853.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42405..42890
                     /gene="skp"
                     /locus_tag="ACHK01_000858"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42405..42890
                     /gene="skp"
                     /locus_tag="ACHK01_000858"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO8095854.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42894..43919
                     /gene="lpxD"
                     /locus_tag="ACHK01_000859"
     CDS             42894..43919
                     /gene="lpxD"
                     /locus_tag="ACHK01_000859"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO8095855.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44024..44479
                     /gene="fabZ"
                     /locus_tag="ACHK01_000860"
     CDS             44024..44479
                     /gene="fabZ"
                     /locus_tag="ACHK01_000860"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO8095856.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44483..45271
                     /gene="lpxA"
                     /locus_tag="ACHK01_000861"
     CDS             44483..45271
                     /gene="lpxA"
                     /locus_tag="ACHK01_000861"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO8095857.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45271..46419
                     /gene="lpxB"
                     /locus_tag="ACHK01_000862"
     CDS             45271..46419
                     /gene="lpxB"
                     /locus_tag="ACHK01_000862"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO8095858.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46416..47012
                     /gene="rnhB"
                     /locus_tag="ACHK01_000863"
     CDS             46416..47012
                     /gene="rnhB"
                     /locus_tag="ACHK01_000863"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO8095859.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47049..50531
                     /gene="dnaE"
                     /locus_tag="ACHK01_000864"
     CDS             47049..50531
                     /gene="dnaE"
                     /locus_tag="ACHK01_000864"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO8095860.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50544..51503
                     /gene="accA"
                     /locus_tag="ACHK01_000865"
     CDS             50544..51503
                     /gene="accA"
                     /locus_tag="ACHK01_000865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO8095861.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51602..53743
                     /gene="ldcC"
                     /locus_tag="ACHK01_000866"
     CDS             51602..53743
                     /gene="ldcC"
                     /locus_tag="ACHK01_000866"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO8095862.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     VQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53800..54189
                     /gene="yaeR"
                     /locus_tag="ACHK01_000867"
     CDS             53800..54189
                     /gene="yaeR"
                     /locus_tag="ACHK01_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8095863.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54254..55552
                     /gene="tilS"
                     /locus_tag="ACHK01_000868"
     CDS             54254..55552
                     /gene="tilS"
                     /locus_tag="ACHK01_000868"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO8095864.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55601..55861)
                     /gene="rof"
                     /locus_tag="ACHK01_000869"
     CDS             complement(55601..55861)
                     /gene="rof"
                     /locus_tag="ACHK01_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO8095865.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55848..56048)
                     /gene="yaeP"
                     /locus_tag="ACHK01_000870"
     CDS             complement(55848..56048)
                     /gene="yaeP"
                     /locus_tag="ACHK01_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO8095866.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56214..56759
                     /gene="yaeQ"
                     /locus_tag="ACHK01_000871"
     CDS             56214..56759
                     /gene="yaeQ"
                     /locus_tag="ACHK01_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO8095867.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSVFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56756..57178
                     /gene="arfB"
                     /locus_tag="ACHK01_000872"
     CDS             56756..57178
                     /gene="arfB"
                     /locus_tag="ACHK01_000872"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO8095868.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57192..57902
                     /gene="nlpE"
                     /locus_tag="ACHK01_000873"
                     /gene_synonym="cutF"
     CDS             57192..57902
                     /gene="nlpE"
                     /locus_tag="ACHK01_000873"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO8095869.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58057..58880)
                     /gene="yaeF"
                     /locus_tag="ACHK01_000874"
                     /pseudo
     CDS             complement(58057..58880)
                     /gene="yaeF"
                     /locus_tag="ACHK01_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58934..60652)
                     /gene="proS"
                     /locus_tag="ACHK01_000875"
     CDS             complement(58934..60652)
                     /gene="proS"
                     /locus_tag="ACHK01_000875"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO8095870.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60764..61471)
                     /gene="tsaA"
                     /locus_tag="ACHK01_000876"
     CDS             complement(60764..61471)
                     /gene="tsaA"
                     /locus_tag="ACHK01_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO8095871.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61468..61872)
                     /gene="rcsF"
                     /locus_tag="ACHK01_000877"
     CDS             complement(61468..61872)
                     /gene="rcsF"
                     /locus_tag="ACHK01_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO8095872.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61990..62805)
                     /gene="metQ"
                     /locus_tag="ACHK01_000878"
     CDS             complement(61990..62805)
                     /gene="metQ"
                     /locus_tag="ACHK01_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO8095873.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62845..63498)
                     /gene="metI"
                     /locus_tag="ACHK01_000879"
     CDS             complement(62845..63498)
                     /gene="metI"
                     /locus_tag="ACHK01_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO8095874.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63491..64522)
                     /gene="metN"
                     /locus_tag="ACHK01_000880"
     CDS             complement(63491..64522)
                     /gene="metN"
                     /locus_tag="ACHK01_000880"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO8095875.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64710..65282
                     /gene="gmhB"
                     /locus_tag="ACHK01_000881"
     CDS             64710..65282
                     /gene="gmhB"
                     /locus_tag="ACHK01_000881"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO8095876.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15416 a        16274 c        18018 g        15632 t
ORIGIN      
        1 gtaatataaa taaattacca tttgcttaat ttgtcaaatg ctcttgtatg taagacttca
       61 cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa tctcaaacag cggccacagc
      121 ataaatccac gattcttcat atcgtagtgc ggaacggtca ggcgttcggt atttatcact
      181 tcattaccaa acagcatgat atcgagatcc agcgtgcgtg gtccccagcg ttcagcttta
      241 cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt tgagtaactc ttccggggcg
      301 agcgaagttt caagcgccac ggcggcgttg aggtaatcgg gttgatcttg cggccccagc
      361 ggtggggtgc ggtaaaacga agaaacggca agaatgtggc tttcagggat atcgcctaat
      421 gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc tatggcaata
      481 tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac
      541 gacgacgcgg tgacggttct tcatccaact cgttaagcat ccctttttga tctggtggcg
      601 cggaaacctg gaactcaccc caccatttca ccagacgctg cagttcagcg ttacgttcaa
      661 cttcagctcg caaggccaac aggtcataag ccgcacggaa cttaggatgc tccagcagtt
      721 tccatgcacg tttaccctga cgtcgggaca tacgcaactg caactgccag atatcgcggg
      781 ttaacgtcgt cagacgtttc gggattgcca gtgaacggca ggcttcgtcc agcacgtcgt
      841 tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg
      901 ccgtctccag cagtgggtac cagaacatgg cggcaaacag gaacgccggg ttcacgcgca
      961 tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc cgctccatcg
     1021 ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc tggaacagat
     1081 gatattcaca cagcagctta taggtttcgt aaccgtagcc cgcttgtagc agtttaagcg
     1141 attcttcaaa caggcgtgcc ggtgggatat cgttcagcag ggtagcgagg cgagggatcg
     1201 gttctgcggt ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt accgcgcgca
     1261 gcatacgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga cggataacgc
     1321 cgtccttcag atccttcata ccgccaacgt aatcacggac ggtaaaatcc gctacgctgt
     1381 aatacaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa
     1441 tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt
     1501 taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca aacatcacat
     1561 gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg cgcacctgct
     1621 caggcgtggc gttagtggtt acgtcaaagt ctttcggctt tttgccaagt aacaggtcgc
     1681 gcacgccgcc gccaaccagc caggcttcgt atcccgcttt gttgagcctg tacattacct
     1741 tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc gggatcaccg
     1801 tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt agcaccttgc
     1861 ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa catcattcag
     1921 gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg ttgaatttac
     1981 aattgccgac tcgggcacgg cggtaagccg ccagtttttg acggctgact gaaggatttg
     2041 ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt gaagtgccgc
     2101 gattagtacc gggcgtggat cgcctttcgg caacgcaggc gcatgattct gcttggaaag
     2161 tttagcgcct tgtggattaa gcgccagcgg cagatgaatg taatctggca ctttccagcc
     2221 aaaaagctgg tacagcgaga tttgccttac tgttggttca atcagatcag ccccacgcac
     2281 tatttctgta acgccctgga aatgatcatc aaccacaaca gccaggttgt aggcgaacaa
     2341 cccatcacgg cgatgaatga taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat
     2401 gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc tggcggatac gcactgcggc
     2461 gttgtctggt ccatgatgca acacccggca atgaccgtcg taaataccgc caatgctttg
     2521 aatacgcgca cgcgtacagg tgcagtaata acttagtcct tgttcatgta accaggcgag
     2581 tgcttcacga taggcgtcgt gacgttgcga ttgccagaga acatcgccgt cccagtgcag
     2641 accgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt cacgaggcgg
     2701 gtcgatatct tctatgcgta ccagccagcg accttgccgg gcgcgagcct gcaaatagct
     2761 gccgagcgcg gcgatcagag agccaaaatg aagctcgccg gaaggagagg gggcgaagcg
     2821 gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga gcatttcccg
     2881 cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt cgcgaatttc
     2941 agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat
     3001 accaatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca cttttttcag
     3061 cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca gttcgaggct
     3121 gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg cttcatcctg
     3181 catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg cttccagaat
     3241 acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct tctcctgata
     3301 tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt tttgcccttc
     3361 ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg aaaaatgagg
     3421 ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac acttgacaac
     3481 tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca gttacttact
     3541 ttactacaat gtaaccggca gtgatttttt aagagccatg ccttcagcag aaatttccgc
     3601 tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt gcgcgtattt
     3661 ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa
     3721 gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt gtttctgacc
     3781 tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg taaccgattt
     3841 cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg
     3901 ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt ctgaaattga
     3961 ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtattga cgcaaataaa
     4021 tgcgccgctc tggctttgag ttagttccca ggtgtgtggg tatttccgtt tggtgttgtc
     4081 tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt
     4141 cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt
     4201 gtaacgctga attagcgtcg cgcgctgtag agggggagaa aattccattg cgacaatcct
     4261 tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac
     4321 gaggaggcgt aaagggtgaa ctccgtcacc gcataagacc agttaaaacc tggcggcggg
     4381 attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg
     4441 ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca tattcgccag ctggattaag
     4501 ccgcgtggag gctgacgcat ccctaaccac accacacgcg aacgcagcca ttgtccggcg
     4561 tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag agaaagcgcc
     4621 ttctctttct cggcgctcac ttcgcctaaa aatgccagag tcagatgcaa attatcggcg
     4681 gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat aatctgttcg
     4741 cggatttctg caggtaagtc gatagcaaag aacagacgtt gcggttcaga catgtgaggc
     4801 actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa ccctctggag
     4861 cgttttgtgt cgtcgttgcc cgttgctgcc gtcttatctg aattacttac cgctctcgat
     4921 tgtgcgccgc aggtattatt aagtgcgccg accggggccg ggaaatcaac ctggctgccg
     4981 ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctgctgga gccgcgtcgt
     5041 ctggcggcgc gtaacgtcgc gcaacgactg gcggagctgc ttaacgaaaa gccaggcgat
     5101 accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg
     5161 gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc cggaactgag cggtgttgga
     5221 ttggtgatcc tcgatgaatt tcatgagcgc agcttgcagg cggatttggc gttggcgctg
     5281 ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat gtcggctacg
     5341 ctggataacg accgcttgca gcaaatgctg ccagaagcgc ccgtcgtcat ctcagaaggg
     5401 cgctcgtttc cggttgaacg ccattattta ccgctgcccg cgcatcagcg ttttgacgaa
     5461 gccgttgcgg tagccaccgc cgaaatgctg cgtcaggaaa gcggatcatt actgttattt
     5521 ttacctggcg tcggagaaat tcagcgtgtg caggagcaac tggcttcgcg catcggcagt
     5581 gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc
     5641 ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt
     5701 ttaaccattg aaggtattcg tctggtggtg gattgtgccc aggagcgtgt ggcgcgtttt
     5761 gatccgcgca cggggcttac gcgactgatt actcaacgcg ttagccaggc atccatgacg
     5821 cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatca gcctgcattt aatcgccaaa
     5881 gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga tcttacaaag cgatctttcc
     5941 ggttttctga tggaattact gcaatgggga tgcagcgatc cggcgcagat gagctggctg
     6001 gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc tgttacaaat gctgggggcg
     6061 ctggagggtg aacggcttag tgcgcagggg caaaaaatgg cagcgctggg taacgatccg
     6121 cgtttagcgg caatgctggt tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa
     6181 attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg cgtggcgttt
     6241 tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta
     6301 cgtggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggggc gtttgccgat
     6361 cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac tggcaaacgg catgggagcg
     6421 atgctcgatg ccaacgacgc gctaagccgc cacgaatggt tgatcgcacc gttattattg
     6481 cagggcagcg cctcgccgga tgcgcggatt ttactggcgt taccggtcga tattgatgaa
     6541 ttagtacaac gctgtccgca gctggtgcag caatctgaca ccgtggaatg ggatgacgcg
     6601 caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc agttgacggt gaaagtgcag
     6661 ccactggcga aaccgtcaga agacgagttg catcaggcga tgcttaacgg cattcgtgaa
     6721 aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt gcgtttgtta
     6781 tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga aagtttattg
     6841 gcgacgctgg aaacgtggct gctgccacat atgactggcg tacattcact acgcggcctg
     6901 aaatcactcg acatttatca ggcactacgc ggattacttg attggggaat gcagcaacgt
     6961 ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat cgccattcgt
     7021 tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aagagatgtt tggcgaggcc
     7081 accaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggagttgct ttcacctgcc
     7141 caaaggccat tacaaatcac acgagatttg agcgccttct ggaaaggagc gtaccgtgag
     7201 gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga cccggcaaat
     7261 actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc ctctccctgt
     7321 gggagagggc cggggtgagg gcatcagcgc gcacgttcac cctcacccta accctctccc
     7381 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7441 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7501 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7561 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7621 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7681 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7741 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgcct
     7801 gcggcagttt atggccgaat ggtcaatctt gagccagaca tgaccatcag caagaacgag
     7861 atggtgaagc tgctggaggc gacccagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     7921 gaatttaccg tgcaggccaa cagcattgag atgattcgcc gtccgtttga tttcccggac
     7981 agtaaagaag gacaggtgcg cgcgcgtctg acctttgatg gcgatcatct ggcgacgatc
     8041 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8101 atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg tttcccggat
     8161 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8221 agtctctact caatcggacg tgcggtgctg gcaaacctga ccgccggacg cacggtacag
     8281 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8341 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8401 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8461 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8521 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8581 cgtaacccaa aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8641 cagattattg atcaagaact ctatgacatg ttgagtgcac gtccgctggg tgttcagcca
     8701 cgcggtgggg tgatctctcc acagccagcc tttatgcaac tggtgcgtca ggagctgcag
     8761 gcgaaattag gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8821 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     8881 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     8941 gttcgtgcga tggtcggtgg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9001 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgactgc cttaagccag
     9061 ccgaaaatct atcgtctgaa tacgtggatt gcggatgcgc caatcgcgct gcgtcagccg
     9121 aatggccagg tctggtcacc gcagaatgat gaccgtcgtt atagcgaaag cggcagagtg
     9181 atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9241 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9301 catccggttc cggcgatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9361 gcgttccaga ccatcgccag cggcggtaac cgtgcaccgc tttctgcact gcgttcggta
     9421 attgcggaag atggcaaggt gctgtatcag agcttcccgc aggcggaacg cgctgttccg
     9481 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg cggtacgggt
     9541 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9601 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9661 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9721 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9781 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9841 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
     9901 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
     9961 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10021 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10081 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10141 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10201 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10261 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggtaagc
    10321 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10381 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcgacag
    10441 tctgctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10501 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10561 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10621 cgcggctttg cggcagaagg ccaaagccag aataactatc tgaatggcct gaagttgcag
    10681 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10741 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt gaatatggtc
    10801 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10861 ctgttccaga ctggttttga ctttagcgat tcgttggatg atgacggtgt ttactcttat
    10921 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    10981 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11041 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11101 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11161 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11221 acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca aaacagcgtt
    11281 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11341 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11401 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11461 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11521 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaataccga tttcgacttc
    11581 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataagca gaaacaaacg
    11641 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct gggcggtcgt
    11701 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11761 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11821 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    11881 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    11941 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12001 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12061 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12121 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12181 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tctgacgctg
    12241 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12301 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12361 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12421 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttc
    12481 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12541 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12601 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12661 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggccgtcatc
    12721 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12781 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12841 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    12901 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    12961 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13021 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacctcggcg ctggatatcg
    13081 cccaccaggt tgatgtgctg tcgctggtgc accgtttaag tcaggagcgt ggcctgacgg
    13141 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13201 tgcgcggcgg tgaaatgatt gttcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13261 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13321 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13381 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgatcc
    13441 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13501 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13561 ggactcagtg atcgacgtcg gtttgcgcac agaacctaac cttgaactgc tgaccgaaat
    13621 gaaaccatcg tttatggtct ggtcggcagg atatggccct tcaccagaaa tgctggctcg
    13681 tattgcgccg gggcgtggat ttaacttcag cgacggcaaa cagccgctgg cgatggcgcg
    13741 taagtcactg acggaaatgg cagatttact taacctgcaa agcgcagcag aaacgcattt
    13801 agcgcactat gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgaacg
    13861 cccgttattg ctgacgacgc ttatcgatcc gcgccatatg ctggttttcg gtccaaacag
    13921 cttgtttcag gaaattcttg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    13981 cttctggggc agtaccgccg tcagcatcga tcgtctggcg gcgtataaag atgttgatgt
    14041 gctctgtttt gatcacgaca acagcaaaga catggacgcg ctaatggcaa ccccgctgtg
    14101 gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacccgcag tctggtttta
    14161 tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg gataacgcca tcggaggtaa
    14221 agcgtgagta aacgaattgc gctttttccg gcgttattgc tggcgctgct ggtgattgtc
    14281 gctacggcgc tgacctggat gaacttttcg caggcgctgc cgcgtagcca gtgggcgcag
    14341 gccgcctggt cgccggatat taacgtcatc gagcagatga tttttcacta cagcttgtta
    14401 ccacgtctgg cgatttctct actggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14461 cagcaagtgc tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc tacaggcgcg
    14521 caactgggga ttaccgtcac cacgctctgg gcgatcccag gtgctatggc gagccagttt
    14581 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14641 cggctgtcgc cggtaacgct gattctcgcg gggctggtag tgagccttta ttgtggggca
    14701 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt cctgtggagc
    14761 accggaacgc tgacgcaaac cgactggggc ggcgttgagc gtttatggcc gcagttgctg
    14821 ggcggcgtga tgctgacgtt actgctactt cgtccgttaa ccctgatggg gcttgatgat
    14881 ggcgtggcgc gcaatctcgg gctggccttg tcgctcgcgc gtctggcggc gctgtcgctg
    14941 gcgatagtca tcagtgcgct gctggtgaac gcagtgggga ttatcggctt tatcgggttg
    15001 ttcgcgccac tgctggcaaa aatgctgggg gcgcggcgtt tgttgccacg gttgatgctg
    15061 gcgtcgctga ttggtgcgtt aattctgtgg ctttctgatc aaatcatcct ctggctgact
    15121 cgcgtgtgga tggaagtttc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15181 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15241 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgctgttg
    15301 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15361 ggggcgctgc tcgatgattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15421 gcgggcgtca tgctggcggt agcgggctgt attattcagc ggctgaccgg caacccgatg
    15481 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15541 tttctggtac cgggtaatgc ctttggctgg ctgttacccg caggcagtct cggggcggcg
    15601 gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15661 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15721 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15781 accgatgcgc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattaccccg
    15841 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcccg agccgtagga
    15901 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    15961 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16021 atgatgggct ttcgacggac gatgccacac atcgtaattt cggcgctggt gggggggtta
    16081 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16141 gggctgctgt caacctttat cggcgcgcca tattttatct atttgttgag aaagcagagc
    16201 cgttaattga atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc gcaaacaccc
    16261 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg gacataaagc
    16321 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16381 gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16441 cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga attccggctt
    16501 cttctgccgc ttccagcaga ccttctgcca gacgtgatgt cagctcatcc aacgtttcgt
    16561 gaacgcccgg ttgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaattgggt
    16621 taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca tccattacat
    16681 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg cccaggcagg
    16741 tgagatccgg ttccacaccg taataatcct gtgcgccagc tagcgctacg cggaagccag
    16801 tcattacttc atcgatgatc agcaatgcgc caaattcgtc gcacagcgcg cgcagacctg
    16861 gcaggaactc tggcagcggt ggaacgcagt tcatattgcc tgccaccggc tcgacgataa
    16921 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc agatcgttat
    16981 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac acccggcgag tttggctggc
    17041 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17101 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc
    17161 tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc gggaccagtt
    17221 cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag cttaaaccac
    17281 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17341 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataggcgc
    17401 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17461 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17521 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17581 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17641 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17701 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17761 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgta gacaactgat tcgccaactt
    17821 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    17881 gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    17941 ggggcgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18001 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18061 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18121 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18181 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18241 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18301 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18361 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18421 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18481 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18541 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18601 ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg tggattgctg
    18661 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18721 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18781 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18841 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    18901 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    18961 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19021 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa accgctatac
    19081 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19141 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19201 ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19261 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19321 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19381 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19441 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19501 gattataccg aaggtctgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19561 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19621 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19681 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19741 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19801 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg gccagtacat
    19861 cacgaatgat cccgccgcca acgccagtaa tgacgcccat gcaaaccgcg attaacggac
    19921 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    19981 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20041 tcagcatgct ggtgaccatt gcaacgacca gatcggtggg atctttcacc caaaataccg
    20101 ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tactgcggtt accacgccca
    20161 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20221 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20281 gcacctgaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag ctgttgtgca
    20341 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20401 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20461 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20521 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20581 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20641 ggtttatcag catattgcgc tttcagttgc gcgtactgat ccagcaggga ttgcgcggct
    20701 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20761 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20821 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggctt cagcgcgaca
    20881 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    20941 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcagcaaa ggcaagttca
    21001 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga
    21061 aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt tctgcaccag
    21121 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21181 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21241 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21301 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21361 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21421 tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa
    21481 tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat cgcccacttt
    21541 caacgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21601 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21661 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21721 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21781 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21841 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    21901 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    21961 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22021 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22081 actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta agccgtctga
    22141 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct tttgaaagca
    22201 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22261 aagcggcgat aaatgactgg tttcgccagc gtttataccc ggaagatgcc gaaagccagc
    22321 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22381 ttaacgagtt gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22441 gcattcgtct ggtgcataca ttgatgagga tgaatctcac ctgggcacag gttggcggta
    22501 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22561 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22621 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22681 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22741 agcagcttta tcatcatttg cacgaggcgt ggggtcagca tgagaaaggt tcgctctttt
    22801 cgctagtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22861 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta ccatacgcgg
    22921 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    22981 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23041 atgtgtttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23101 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23161 tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg cttattccac aaactctcga
    23221 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23281 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23341 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23401 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcgcg
    23461 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23521 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23581 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23641 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23701 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23761 ctccgttctg ccaggaaggt tcaccgttcc agagctctcc gttctgccag gggggccagg
    23821 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    23881 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    23941 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24001 ctgatatcgc gctgattcag atccagaacc cgaaaaacct gaccgcaatt aagatggcgg
    24061 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggcctgg
    24121 gagagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgccgaaa
    24181 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24241 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24301 gcaacatcgg tatcggtttt gctatcccga gcaacatggt gaaaaacctg acctcgcaga
    24361 tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24421 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24481 tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24541 acggtaagcc aatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24601 gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg aatctggaac
    24661 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggtg
    24721 ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg aaaacgggca
    24781 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg aaccagcagg
    24841 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    24901 tgaacattca gcgcggcgac agctccatct acctgttaat gcagtaatct ccctcaaccc
    24961 cttcctgaaa acgggaaggg gttctcctta caatctgtga acttcaccac aactccatac
    25021 atcttcatca tcctttaggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25081 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25141 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgtggg
    25201 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25261 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tggcacgtca tctgcacggt
    25321 gtgcggcaag ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25381 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25441 atgatgctgg aacagtcgcg gttgatgcac ttgttggctc aggatagccg tttgcgggag
    25501 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttac tgaatgggcg
    25561 caacggctgg ggatcgatct caatcaaccg cgagtggtgg ctattgttga ggtcgacagc
    25621 ggtcagcttg gtgtggacag cgcaatggcg gagttacaac aactgcaaaa cgcgctgact
    25681 acgcccgagc gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa
    25741 ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg agttgaacaa
    25801 ctgattaccc gcatgaaaga gtacggccag ctgcgttttc gcgtttcact gggcaactat
    25861 tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg
    25921 ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat gttacctgtg
    25981 ttactcgaca gtttgcgtgg cgactggcag gccaacgaac tggcgcgacc gctggcgcgg
    26041 ctgaaagcga tggacaataa cggcttgctg cgacgaacgc tggcggcgtg gtttcgtcac
    26101 aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc atcgtaatac tctggagtat
    26161 cggcttaatc gtatatcgga actgaccgag cttgatttgg gtaattttga tgacaggttg
    26221 ctgctgtatg tggcgttgca gctggatgaa gagcggtagg ttatgcgtta aggtcgaggc
    26281 ggcgctggct cattcccctc agcccgaagg gtagagggga atgtccgagt taaccgtcag
    26341 ttcggcatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg atcttattgg
    26401 ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc
    26461 tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga ttcgggctga
    26521 tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt ttacgctgac
    26581 gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc tggaaataga
    26641 ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt tcttcaggat
    26701 tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat gagtcagtaa
    26761 attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc gcagaaagcc
    26821 tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct ttattgtggc
    26881 gatattccat gggatggatt agcacattta tcttcgtttt gcgaattaca tgacagtccg
    26941 aaacaagatg aaaaagtatt taaaaaaggg gggaagaatg agtaggtcga attattctga
    27001 gccagaaagc atacgggcag cattatgctg cccgtatatg tactcttaat cgatggtacg
    27061 cagcagttcg ttaatgccga ctttgccgcg agttttcgcg tcaactttct taacgataac
    27121 cgcacagtag aggctgtatt tgccatcttt cgacggcaga ttacctgaaa caaccacaga
    27181 ccccgccgga acgcgaccgt agtggatttc gccggtttca cggtcgtaaa tacgggtgct
    27241 ctgaccaatg tatacgccca tagagatgac ggaaccttct tcgacaatca ccccttcaac
    27301 cacttcggag cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt tagcctgcag
    27361 cggctccagc acgccgccga tgcccacgcc accggaaagg tggacgtttt taccaatctg
    27421 cgcacaagaa ccgacggtcg cccaggtatc aaccatcgtg ccttcatcaa catatgcgcc
    27481 gatgttgacg taagacggca tcagcacggt gttacgggca ataaatgcac cctgacgtac
    27541 cgccgctggt ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt cgtagtcggc
    27601 gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca cctgattatc
    27661 attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca cccactgacc
    27721 gtcaattttt tccgctacac gcagtgcacc ggaatccagc agggcgatca cctgatttac
    27781 cgcttcgcgg gtaacggtgt ctgcgttggc tggcgtgatc tcggcacggc gttcaaaagc
    27841 ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc agtaaacaca
    27901 tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa
    27961 ctcgttatta agtgcacgcc ggtcagcggt ggcaattatg aataaatctt ctactcgctc
    28021 gccaatggtt gtaattcggg caccatgaag cgaaattccc agatcggcaa aaattttccc
    28081 gactctcgcc agcagtcctg gctggtcgag cgcgataagc tcaaggaacg atttgcggtc
    28141 ggtatgggtc ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta atttggcggg
    28201 ttggcgacgg ggctgcggtg gctgccagct actttgcgtc agtacttgct ccagaccaaa
    28261 ccgaataacc tcatgacgat ctgcggacag cgggctgcca tcgggttcca gcacgataaa
    28321 ggtatccatc gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt
    28381 gcggcggtct aattcggcac agacggcggc aaacagatag gggcggtccg ggctccagat
    28441 aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc aataccagcg gtttgcttaa
    28501 atcatgctgt aataaatggc gggcatgcca ggccagttga tttgggctat ggcggacaaa
    28561 atagttagca cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga tgttatccat
    28621 gcgcagtagt gccagtgcct ggagttggtg atggcgaacc cgttcgcgca tatccggcgt
    28681 gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc gcaacagact
    28741 ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca
    28801 taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct tgatgacttc
    28861 agggtcctga atatcgcggc gttgtgctgt caccgacatc agcaagtgct ggcgaaccag
    28921 ccaggcaacc agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa aatgcactac
    28981 atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat cgtgaaacag
    29041 cgcggcgatg aaaatcagct cagttgacgg caggcgcggc cagacgtcca cacacaacgg
    29101 atggcgctgg cgcgtttctt cactggcaaa actctccagt ttcagcatca cgcggatagt
    29161 atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga cgatatgcga
    29221 ccattgcggc atatacgcgc cgagcacgct atggcgatgc attggcaata gcccgcgccg
    29281 caccgctccg gggtgacgca gaatgctcaa aaacagtttt cgtgcttccg gaatattaca
    29341 cagcggttgt tgcagatggc gacgggcatg gcgtaactgg cgcagcgtgg tggagtaaat
    29401 gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg cttccggctg
    29461 gcgcataaat agtgtttcat cacgcaggtc gattagcgta ccgcgtagct gaaactcatc
    29521 gtcgattgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat cgaacagttg
    29581 cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat ccttcatcat
    29641 ccgctcgacc ggttcgttgc cttcaccact gtaattcaga cgctgagcga cgctaagttg
    29701 gcgatcgaat aacagacgat tatcgtaacg gctgacgacc agatgcaggg caaagcgaat
    29761 acgccacagt atatgcagac attcgtttaa ttccgcccgc tccgctgagg ttaagaagcc
    29821 aaacccaacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca cccattgcag
    29881 agtgtggata tcgcgcaagc cgccagggct acttttgatg tctggttcaa gattgtagct
    29941 ggtgccgtgg taacgctgat ggcgctggtt ctgttcttca actttcgccg cgtagaactt
    30001 gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc agttcgagga agagcgcaac
    30061 atcgccaatt aataagcggg attcgattaa attggtggcg acggttaaat ccgataaccc
    30121 ttccagcatg cactcttcaa gcgtgcgcac actatgaccg acttccagct ttacatccca
    30181 gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct ttttacggct
    30241 taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt agccaccgac
    30301 ggcgaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata atcgttgcag
    30361 gagctggtcg ataaactcgg tgcgcgcctc aatcaactgt tctgcagaga tcccattgtc
    30421 aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc caccgacggt
    30481 taattcatca cggggccaga cgcatggatt ttgcggttga ccgggcaggg tggggagagc
    30541 ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag attaaattat
    30601 cgcattaaaa aagccggcat tcgccggctt catcttattc gtcgtgcgag attatcgccg
    30661 ggatggtgtc atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc accacaatag
    30721 tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag ccatctttca
    30781 tggtgcggat ctctttttta cccgcgttga ccattggctc aatggtgaac gtcatccccg
    30841 gtttcagcac gacgttggtt tcacgggagt catagtgcag cacctgcggt tcttcatgga
    30901 agccgcgacc aataccgtgt ccacaatatt cacgaacgac ggagaagcct tctgcttcga
    30961 caaacttctg aatcgccgca ccgatttcgc gcaggttaat gcctggtttg accatgcgca
    31021 gcgccaggta caggctttct tgcgtgatgc ggcacagacg ttcgcccatg atggtcggct
    31081 taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt acggtgacat
    31141 caatgttaac gatatcgcca tctttcagca gcttagcatc gtccgggata ccgtggcaca
    31201 ccacttcatt aatagagatg caaacggatt tcggatagcc gtgatagccg aggcaggcag
    31261 aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccagc tcgccggtgc
    31321 tgacgcccgg tttaacatac ggttcgatca tctccagcac ttcggcagcc agtcggccag
    31381 cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt aattctgtcc
    31441 atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat cacacccggg
    31501 caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc tataatagaa
    31561 aattcgacat ctgatgctgt acacagcgcc aacaattatt ggtgtccatg acgtatttgt
    31621 ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac ggaagcgaca
    31681 atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc cctttggggt
    31741 cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt ttaatcatgg
    31801 caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac cagacccgtt
    31861 actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt cacatcatca
    31921 accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac aagattgctt
    31981 ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag
    32041 acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc ggtatgctga
    32101 ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa actcagtctc
    32161 aggacggtac tttcgaaaag ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg
    32221 agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg gacgctctgt
    32281 ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac ctgggtattc
    32341 cggtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc gttatcccgg
    32401 gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct gcaaccgtac
    32461 gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta gaagctgagt
    32521 aataaggctt gataactccc ccaaaatagt tcgagttgca gaaaggcggc aagctcgaga
    32581 attcccggga gcttacatca gtaagtgacc gggatgagcg agcgaagata acgcatctgc
    32641 ggcgcgaaat atgaaggggg agagccctta tagaccaggt agtacacgtt tggttagggg
    32701 gcctgcatat ggcccccttt ttcactttta tatctgtgcg gtttaatgcc gggcagatca
    32761 catctccgag gattttagaa tggctgaaat taccgcatcc ctggtaaaag agctgcgtga
    32821 gcgtactggc gcaggcatga tggattgcaa aaaagcactg actgaagcta acggcgacat
    32881 cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg
    32941 caacgttgct gctgacggcg tgatcaaaac caaaatcgac ggcaactacg gcatcattct
    33001 ggaagttaac tgccagactg acttcgttgc aaaagacgct ggtttccagg cgttcgcaga
    33061 caaagttctg gacgcagctg ttgctggcaa aatcactgac gttgaagttc tgaaagcaca
    33121 gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt gaaaacatca acattcgccg
    33181 cgttgctgcg ctggaaggcg acgttctggg ttcttatcag cacggtgcgc gtatcggcgt
    33241 tctggttgct gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc
    33301 tgcaagcaag ccagaattca tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga
    33361 ataccaggta cagctggata tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa
    33421 aatggttgaa ggccgcatga agaaattcac cggcgaagtt tctctgaccg gtcagccgtt
    33481 cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg ctgaagtgac
    33541 tggcttcatc cgcttcgaag tgggtgaagg catcgagaaa gttgagactg actttgcagc
    33601 agaagttgct gcgatgtcca agcagtctta attatcgaaa aggagccgcc tgagggcggc
    33661 ttctttttgt gcccatcttg taaattcagc taacccttgt ggggctgcgc tgaaaagcga
    33721 cgtacaatgt cgctagtatt aattcatttc aatcgttgac agtctcagga aagaaacatg
    33781 gctaccaatg caaaacccgt ctataaacgc attctgctta agttgagtgg cgaagctctg
    33841 cagggcactg aaggcttcgg tattgatgca agcatactgg atcgtatggc tcaggaaatc
    33901 aaagaactgg ttgaactggg tattcaggtt ggtgtggtga ttggtggggg taacctgttc
    33961 cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca catggggatg
    34021 ctggcgaccg taatgaacgg cctggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac
    34081 gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg acagctacag ctgggcagaa
    34141 gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac aggtaacccg
    34201 ttctttacca ccgactcagc agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg
    34261 ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc cggcgaaaga tccaaccgca
    34321 accatgtacg agcaactgac ttacagcgaa gtgctggaaa aagagctgaa agtcatggac
    34381 ctggcggcct tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt caacatgaac
    34441 aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa
    34501 taattcccgt gatggataaa taagggtaag attccgcgta agtatcgcgg gggcgttagt
    34561 ctggttataa ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc agcaaccgga
    34621 gcgtacaaaa gtacgtgagg atggcgagca ctgcccgggg ccaaaatggc aaataaaata
    34681 gcctaataat ccagacgatt acccgtaata tgtttaatca gggctatact tagcacactt
    34741 ccactgtgtg tgactgtctg gtctgactga gacaagtttt caaggattcg taacgtgatt
    34801 agcgatatca gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc gttcaaaacc
    34861 caaatcagca aaatacgcac gggtcgtgct tctcccagcc tgctggatgg cattgtcgtg
    34921 gaatattacg gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt agaagattcc
    34981 cgtacactga aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga aaaagcgatt
    35041 atggcgtccg atcttggcct gaacccgaac tctgcgggta gcgacatccg tgttccgctg
    35101 ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg tgaagcagaa
    35161 caagcgcgtg ttgcagtacg taacgtgcgt cgtgacgcga acgacaaagt gaaagcactg
    35221 ttgaaagata aagagatcag cgaagacgac gatcgccgtt ctcaggacga tgtacagaaa
    35281 ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga agcagaactg
    35341 atgcagttct gatttcttga acgacaaaaa cgccgctcag tagatccttg cggatcggct
    35401 ggcggcgttt tgctttttat tctgtctcaa ctctggatgt ttcatgaagc aactcaccat
    35461 tctgggctcg accggctcga ttggttgcag cacgctggac gtggtgcgcc ataatcccga
    35521 acacttccgc gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg tagaacagtg
    35581 cctggaattc tctccccgct atgccgtaat ggacgatgaa gcgagtgcga aacttcttaa
    35641 aacgatgcta cagcaacagg gtagccgcac cgaagtctta agtgggcaac aagccgcttg
    35701 cgatatggca gcgcttgagg atgttgatca ggtgatggca gccattgttg gcgctgctgg
    35761 gctgttacct acgcttgctg cgatccgcgc gggtaaaacc attttgctgg ccaataaaga
    35821 atcactggtt acctgcggac gtctgtttat ggacgccgta aagcagagca aagcgcaatt
    35881 gttaccggtc gatagcgaac ataacgccat ttttcagagt ttaccgcaac ctatccagca
    35941 taatctggga tacgctgacc ttgagcaaaa tggcgtggtg tccattttac ttaccgggtc
    36001 tggtggccct ttccgtgaga cgccattgcg cgatttggca acaatgacgc cggatcaagc
    36061 ctgccgtcat ccgaactggt cgatggggcg taaaatttct gtcgattcgg ctaccatgat
    36121 gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt aacgccagcg ccagccagat
    36181 ggaagtgctg attcacccgc agtcagtgat tcactcaatg gtgcgctatc aggacggcag
    36241 tgttctggcg cagctggggg aaccggatat gcgtacgcca attgcccaca ccatggcatg
    36301 gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa gtgcgttgac
    36361 atttgccgca ccggattatg atcgttatcc atgcctgaaa ctggcgatgg aggcgttcga
    36421 acaaggtcag gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg ttgctgcttt
    36481 tcttgcgcaa caaatccgct ttacggatat cgctgcgttg aatttatcgg tactggaaaa
    36541 aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg cgaacgcgcg
    36601 tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga ggataatccg gctacagaga
    36661 gtcgcgctat ttgttagcgt agggcttcag tgatatagtc tgcgccatct gatcgtaagt
    36721 agttggcttt ataaggtcag gtatgccgtg gttttacacg gctttttttt gtataggctt
    36781 cagtattcct gagtaccgta aaccctgtca gggaataaaa aacgcgtgat gttgtctgct
    36841 actcaaccac ttagcgaaaa attgccagcg catggctgcc gtcatgttgc gatcattatg
    36901 gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg gcataaagcc
    36961 ggggcaaaat ccgtccgccg ggctgtctct tttgcggcca acaacggtat tgaggcgtta
    37021 acgctgtatg cctttagtag tgaaaactgg aaccgaccag cgcaggaagt cagtgcgtta
    37081 atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg acataacgtg
    37141 cgtctgcgta ttattggcga taccagtcgc tttaactcgc gtttgcaaga acgtattcgt
    37201 aaatctgaag cgctaacagc cgggaatacc ggtctgacgc tgaatattgc ggcgaactac
    37261 ggtggacgtt gggatatagt ccagggagtc aggcaactgg ctgaaaaggt gcagcaagga
    37321 aacctgcaac cagatcagat agatgaagag atgctaaacc agcatgtctg tatgcatgaa
    37381 ctggcccctg tagatttagt aattaggact gggggggagc atcgcattag taactttttg
    37441 ctttggcaaa ttgcctatgc cgaactttac tttacagatg ttctctggcc cgatttcgat
    37501 gaacaagact ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg tttcggcggc
    37561 accgagcccg gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta tcgcctgata
    37621 tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc gccggtgggg
    37681 ttcgccatcg taacgctggt ggtctgcatg ctggcagcgt gggaatgggg acagcttagc
    37741 ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt attgttggcg
    37801 ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc atcaaccgct ggttgaaatc
    37861 tcactttggg cttcgctggg ttggtggatt gtcgcgctat tgctggtgct gttttaccca
    37921 ggttccgcag caatctggcg taactctaaa acattgcgcc ttatttttgg cgtgctaacc
    37981 attgttccct tcttctgggg catgctggcg ttacgggcct ggcactacga cgagaatcat
    38041 tacagtggcg caatatggct gctctatgtc atgatcctgg tatggggcgc tgactctggc
    38101 gcatatatgt ttggcaaatt gtttggtaaa cataagctgg caccgaaggt ttctccgggt
    38161 aaaacctggc aaggctttat aggtggacta gcgaccgcag cggtaatctc atggggttat
    38221 ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac tcatttgctc tattgtcgca
    38281 gcgttagcct cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga agcaggaatt
    38341 aaggacagcg gtcatttaat tccaggacac ggtggtattt tagatcgtat tgatagcctg
    38401 acggctgcgg taccggtctt tgcttgcttg ttgttactgg tattcaggac gctttaacgg
    38461 aaggtaatat gctgagtttt ctctgggatt tggcttcgtt catcgttgca ctgggtgtac
    38521 ttatcaccgt gcatgaattt ggtcatttct gggttgcccg gcgttgtggt gttcgcgttg
    38581 agcgtttctc aatagggttt ggtaaggcgc tctggcggcg aactgataag ctcggcaccg
    38641 aatatgttat cgccctgatc ccgctgggcg gttatgtcaa aatgctggat gagcgcgcag
    38701 aaccggtcgt tccggaactc cgccaccatg ccttcaataa taaatctgtt ggccaacgag
    38761 cggcgattat tgccgcaggt ccggttgcaa acttcatttt tgctatcttt gcctactggc
    38821 tggtttttat tattggtgtg cctggcgtac gtccggtggt tggtgaaata gcagccaatt
    38881 cgatagctgc ggaagcacaa attgcaccag gtacggaact aaaagccgta gatggtatcg
    38941 aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga taaaattggc gatgaaagca
    39001 ccaccattac agtagcgcca tttggcagcg atcaacggcg ggatgtaaag ctcgatttac
    39061 gtcactgggc gtttgagcct gataaagaag atccggtatc ttcgctgggg attcgtcctc
    39121 gtgggccgca aattgaacct gtactggaaa atgtgcagcc aaactcggcg gcaagcaagg
    39181 caggtttgca agcaggcgac aggatcgtta aagtcgatgg tcagccctta acgcagtggg
    39241 tgacctttgt gatgcttgtc cgggataacc cgggtaaatc cttagcgtta gaaatcgaaa
    39301 ggcagggaag tcccttgtct ttgacattaa tcccggagag taaaccgggt aatggtaaag
    39361 cgattggttt tgtcggtatt gagccgaaag tcattccttt gccagatgag tataaagttg
    39421 tacgccagta tgggccgttc aacgccatcg tcgaagccac ggacaaaacg tggcagctga
    39481 tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa ctgaacaacc
    39541 tcagtgggcc gatctctatc gccaaggggg ctgggatgac agcggaactc ggggtagttt
    39601 attacctgcc gtttcttgcg cttattagcg tgaacttagg gataattaac ctgtttccgt
    39661 tgcccgtact tgacgggggg catctgctgt tccttgcgat cgaaaagatc aagggcggac
    39721 cggtatccga gcgggttcaa gacttttgtt atcgcattgg ctcgattctg ctggtgctgt
    39781 taatggggct tgcacttttc aatgatttct ctcggttatg agagttagtt aggaagaacg
    39841 cataataacg atggcgatga aaaagttgct catagcgtcg ctgctgttta gcagcgccac
    39901 cgtatacggt gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc ttcagcgtgt
    39961 cgccgttggt gcggccctcc tcagtatgcc ggtgcgcaca ggcgacacgg ttaatgatga
    40021 agatatcagt aataccattc gcgctctgtt tgctaccggc aactttgagg atgttcgcgt
    40081 ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa cgtccgacca ttgccagcat
    40141 tactttctcc ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc tcgaggcttc
    40201 tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt gccgatatcg agaaaggtct
    40261 ggaagacttc tactacagcg tcggtaaata tagcgccagc gtaaaagctg tcgtgacccc
    40321 gctgccgcgc aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt cagctgaaat
    40381 ccagcaaatt aacattgttg gtaaccatgc tttcaccacc gacgaactga tctctcattt
    40441 ccaactgcgt gacgaagtgc cgtggtggaa cgtggtaggc gatcgtaaat accagaaaca
    40501 gaaactggcg ggcgaccttg aaaccctgcg cagctactat ctggatcgcg gttatgcccg
    40561 tttcaacatc gactctaccc aggtcagtct gacgccagat aaaaaaggta tttacgtcac
    40621 ggtgaacatc accgaaggcg atcagtacaa gctttctggc gttgaagtga gcggcaacct
    40681 tgccgggcac tccgctgaaa ttgagcagct gactaagatc gagccgggtg agctgtataa
    40741 cggcaccaaa gtgaccaaga tggaagatga catcaaaaag cttctcggtc gctatggtta
    40801 tgcctatccg cgcgtacagt cgatgcccga aattaacgat gccgacaaaa ccgttaaatt
    40861 acgtgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt ttgaaggtaa
    40921 cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag gtgcatggct
    40981 ggggagcgat ctggtcgatc agggtaagga gcgtctgaat cgtctgggct tctttgaaac
    41041 tgtcgatacc gatacccaac gtgttccggg tagcccggac caggtagatg tagtctacaa
    41101 ggtgaaagaa cgtaacaccg gtagcttcaa cttcgggatt ggttacggta ctgaaagtgg
    41161 cgtgagcttc caggctggtg tgcagcagga taactggtta ggtacaggtt atgctgttgg
    41221 tatcaacggg accaaaaacg attaccagac ctatgctgaa ctgtcggtaa ccaacccgta
    41281 cttcactgta gatggtgtaa gcctcggtgg tcgtctcttc tataatgact tccaggcaga
    41341 tgacgccgac ctgtccgact ataccaacaa gagttatggt acagacgtga cgttgggctt
    41401 cccgatcaac gaatataact cgctgcgtgc aggtctgggt tatgtacata actccctgtc
    41461 caacatgcag cctcaggttg cgatgtggcg ttatctgtac tctatgggtg aacatccgag
    41521 cacctctgat caggataata gcttcaaaac ggacgacttc acgttcaact atggttggac
    41581 ctataacaag cttgaccgtg gttacttccc gacagatggt tcacgtgtca acctgaccgg
    41641 taaagtgacc attcctggat cggataacga atactacaaa gtgacgttag acacggcgac
    41701 ttatgtgccg atcgatgacg atcacaaatg ggttgttctg gggcgtaccc gctggggtta
    41761 tggtgatggt ttaggcggca aagagatgcc gttctacgag aacttctatg ccggtggttc
    41821 cagcaccgtg cgtggcttcc agtccaatac aattggtccg aaagcagttt acttcccgca
    41881 tcaggccagt aattatgatc cggactatga ttacgaatgt gcgactcagg acggcgcgaa
    41941 agacctgtgt aaatcggatg atgctgtagg cggtaacgcc atggcggttg ccagcctcga
    42001 gttcatcacc ccgacgccgt ttattagcga taagtatgct aactcggttc gtacttcctt
    42061 cttctgggat atgggtaccg tttgggatac aaactgggat tccagccaat attctggata
    42121 tccggactat agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat tacaatggat
    42181 gtccccattg gggccgttgg tgttctccta cgcccagccg ttcaaaaagt acgatggaga
    42241 caaggcagaa cagttccagt ttaacatcgg taaaacctgg taagtgttct ccacaaagga
    42301 atgtagtggt agtgtagcga tgactttagg cgatcaatat aagatcgccg ggccacgcaa
    42361 agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa aagtggttat
    42421 tagctgcagg tctcggttta gcactggcaa cttctgctca ggcggctgac aaaattgcaa
    42481 tcgtcaacat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt tctaacacgc
    42541 tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc gatctgcagg
    42601 ctaaaatgaa aaagctgcag tccatgaaag cgggcagcga tcgcactaag ctggaaaaag
    42661 acgtgatggc tcagcgccag acttttgctc agaaagcgca ggcttttgag caggatcgcg
    42721 cacgtcgttc caacgaagaa cgcggcaaac tggttactcg tatccagact gctgtgaaat
    42781 ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt gcttacaaca
    42841 gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa gtaatgcctt
    42901 caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt gatggcgata
    42961 tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt acgttcatgg
    43021 ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt gtcatgaccc
    43081 aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc tacctgactt
    43141 acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac attgcaccca
    43201 gtgcggtgat cgacgcgacg gcaaagctgg gtaacaacgt atcgattggc gctaacgcgg
    43261 tgattgagtc cggcgttgaa ctgggcgata acgtgattat cggtgccggt tgcttcgtag
    43321 gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc atttaccatg
    43381 agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc gcagacggct
    43441 ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt cgcgtaatta
    43501 ttggcgatcg cgtggagatc ggtgcctgca caaccatcga tcgcggcgcg ctggatgaca
    43561 ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat aacgtcgtga
    43621 ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg aaaattggtc
    43681 gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata tgcgacaaag
    43741 tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc gtctattcct
    43801 caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg gtgatgaaca
    43861 ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa caagactaac
    43921 gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt gcggcaggcc
    43981 gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta ctaacactca
    44041 tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt tcttactggt
    44101 ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa atgtctctgt
    44161 caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg gtgtgctgat
    44221 tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag gaaaactgga
    44281 accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc gcccggtcgt
    44341 gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg gcctgacccg
    44401 ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc gaagcaacga tgatgtgtgc
    44461 tcgtagccgg gaggcctgat aagtgattga taaatccgcc tttgtgcatc caaccgccat
    44521 tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt gtatcgttgg
    44581 accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg tgaatggtca
    44641 tactaaaatt ggccgcgata atgagattta tcagttcgcc tccatcggcg aagttaacca
    44701 ggatctgaaa tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta accgcattcg
    44761 cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga aggtgggcag
    44821 cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag gtaaccgctg
    44881 tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact tcgcgatcat
    44941 cggcggcatg accgcagtcc atcagttctg catcattggt gcgcacgtga tggttggcgg
    45001 ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta accacgcaac
    45061 gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg aggcgattac
    45121 cgctatccgc aatgcgtata agctgattta tcgtagcggt aaaacgctcg atgaagtgaa
    45181 accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta ccgatttctt
    45241 tgcacgctca acgcgcggtc tgattcgtta atgactgaac agcgtccatt aacgattgcc
    45301 ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg cgctctgaaa
    45361 gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca ggctgaaggc
    45421 tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga agtgctcggt
    45481 cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg cgaactgaag
    45541 ccagatattt ttgttggtat tgatgcgcct gacttcaata ttactcttga aggtaacctc
    45601 aaaaagcagg gtatcaaaac cattcattac gtcagtccat ccgtctgggc gtggcgacag
    45661 aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct gcctttcgaa
    45721 aaagcgtttt atgacaaata caatgtaccg tgccgcttta tcggtcatac catggctgat
    45781 gccatgccgt tagatccaga taaaaatgcc gcccgtgatg tgctggggat ccctcacgat
    45841 gcccactgcc tggcgttgct accagggagc cgtggtgcag aagttgaaat gcttagtgcc
    45901 gatttcctga aaacggccca gcttttgcgc cagacatatc cggatctcga aatcgtggtg
    45961 ccactggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctga agtcgcgcca
    46021 gacctttcag ttcatttgct ggatgggatg ggccgtgagg cgatggtcgc cagcgatgcg
    46081 gcgctactgg cgtcgggtac ggcagccctg gagtgtatgc tggcgaaatg cccgatggtg
    46141 gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt gaaaactgat
    46201 tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagaatt attgcaggaa
    46261 gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa cgggaaaacc
    46321 agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat
    46381 gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtt tatccgcaca
    46441 cgcagctggt tgcgggtgtg gatgaagtcg gacgtgggcc gttagttggc gcggtcgtca
    46501 ccgctgcggt gatccttgac ccggcgcgcc cgattgccgg gctgaatgat tccaaaaagc
    46561 tgagcgaaaa acgccgtctg gcgctctatg aagagatcaa agagaaagcg ttgagctgga
    46621 gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa cattcttcat gcgaccatgc
    46681 tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg ttgattgatg
    46741 gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggc gatagccgcg
    46801 taccggaaat cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc gaaatggcgg
    46861 cgctggatat tgttttcccg caatatggtt ttgcccaaca caaagggtac ccaaccgctt
    46921 ttcatctgga aaaactggct gaacacggcg cgaccgaaca ccatcggcgc agctttgggc
    46981 ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa gtaaaccgga
    47041 atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac tactcgatga
    47101 tcgatggcct ggccaaaacc gcaccgttgg taaaaaaggc ggcggcgttg ggtatgccag
    47161 cactggcgat caccgatttc accaaccttt gtggtctggt gaagttctac ggagcgggac
    47221 atggcgcagg gattaagcct atcgtcgggg cagattttaa cgtccagtgc gacctgctgg
    47281 gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat cagaatctga
    47341 cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg atcatcgatc
    47401 gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcgga cgcatgggcg
    47461 acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt gtcgcgtttt
    47521 atgaagaaca cttcccggat cgctattttc ttgagctgat ccgcaccggc aggccggatg
    47581 aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg cccgtcgtgg
    47641 cgaccaacga cgtgcgcttt atcgacagca gcgactttga cgcacacgaa atccgcgtcg
    47701 cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat tcgccgcagc
    47761 aatatatgcg tagcgaagag gagatgtgtg agctgtttgc cgacatcccc gaagcccttg
    47821 ccaacaccgt tgagatcgcc aaacgctgta acgtaaccgt gcgtcttggt gaatacttcc
    47881 tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag cgtgcaaaag
    47941 agggcctgga agagcgtctg gcctttttat tccctgatga ggaagaacgt cttaagcgcc
    48001 gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag atgggcttcc
    48061 cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac ggcgtaccgg
    48121 tagggccagg ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg aaaatcaccg
    48181 acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg gaacgtgtct
    48241 ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt atcgagcacg
    48301 tagcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt acgatggcgg
    48361 cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc tttgtcgatc
    48421 gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg tttgaagccg
    48481 agccgcagct gccggaaatc tacgaagcgg atgaagaagt taaggcgctg atcgacatgg
    48541 cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg gtggttatcg
    48601 caccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc aaacatccgg
    48661 tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc gacttccttg
    48721 gtttgcgtac gctcaccatc atcaactggg cgctggagat gatcaacaag cggcgggcga
    48781 agaatggcga gccgccgctg gatatcgccg cgatcccgct ggatgacaag aaaagcttcg
    48841 acatgctgca acgctcggaa accactgcgg tattccagct tgaatcgcgc ggcatgaagg
    48901 acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgccctg gtggcactgt
    48961 tccgccccgg tccgttgcaa tcagggatgg tggataactt tatcgaccgt aaacatggtc
    49021 gtgaagagat ctcctatccg gacgtgcagt ggcagcatga aagcttgaaa ccggtactgg
    49081 agccaaccta cggcatcatc ctgtatcagg aacaggtcat gcagattgcc caggtgcttt
    49141 ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc gatggggaag aaaaagccgg
    49201 aagagatggc taagcaacgt tctgtatttg ctgaaggtgc agaaaagaac ggaatcaacg
    49261 ctgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgctggttac ggatttaaca
    49321 aatcgcactc tgcggcctat gctttggtgt catatcaaac gttatggctg aaagcacact
    49381 atcctgcgga gtttatggcg gcggtaatga ccgccgatat ggacaacacc gagaaggtgg
    49441 tgggtctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca gatataaact
    49501 ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt attggcgcga
    49561 tcaaaggggt cggtgaaggt ccgattgagg ccatcatcga agcccgtaat aaaggcggct
    49621 acttccgcga actgtttgat ctctgcgccc gtaccgacac caaaaagttg aaccgtcgcg
    49681 tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacat cgcgcggcgc
    49741 tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa gcggaagcta
    49801 tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt gaacaatcct
    49861 acgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt gaaacgttag
    49921 gcctgtacct gaccggacac cctatcaacc agtatttaaa agagattgag cgttatgtcg
    49981 gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc acggctgcgg
    50041 ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc ggtatctgca
    50101 cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc ctggataaat
    50161 accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc agctttgatg
    50221 acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac gaagcccggg
    50281 aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat gaccagcttt
    50341 taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca gtacatctct
    50401 actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg cgtgtctctc
    50461 cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag gtggaactgg
    50521 agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa cagccgattg
    50581 cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat gagaaactgg
    50641 atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa ctgacacgta
    50701 aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat ccacagcgtc
    50761 cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg gctggcgacc
    50821 gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtcttgac ggtcgtccgg
    50881 tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc cgtaactttg
    50941 gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg gctgaacgct
    51001 ttaagatgcc aatcatcacc tttatcgaca ccccgggggc ttatccgggc gtgggcgcag
    51061 aagagcgcgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct cgccttagcg
    51121 taccgactat ttgtaccgtt atcggtgaag gtggttctgg cggcgcgctg gcgattggcg
    51181 tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg ccggaaggtt
    51241 gtgcgtccat tctgtggaag agcgctgata aagcgccgct ggcggctgaa gcgatgggta
    51301 tcatcgctcc gcgtctgaaa gaactaaaac tgatcgactc catcatcccg gaaccgctgg
    51361 gtggtgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa ctgctggcgg
    51421 atctggccga tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt tatcagcgcc
    51481 tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac ttcggtggcc
    51541 cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc aggaggaaca
    51601 catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg agcccatcaa
    51661 agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac aaaacagcgt
    51721 tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt ttgactggga
    51781 tgagtacagt ctcgatttat gcagcgatat caatcaactt aatgaatatc tcccgcttta
    51841 tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc ggatggcgct
    51901 ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta tgcgtcagta
    51961 caccgacgaa tatcttgata acattacacc gccgttcacg aaagccttgt ttacctacgt
    52021 caaagagcgg aagtacacct tttgtacgcc ggggcatatg ggcggcaccg catatcaaaa
    52081 aagcccggtt ggctgcctgt tttatgattt tttcggcggg aataccctca aggctgatgt
    52141 ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac accgggccac acctggaagc
    52201 ggaagagtac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta ccaacggaac
    52261 atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta cgctgttgat
    52321 cgaccgcaat tgtcataaat cgctggcgca tctgttgatg atgaacgatg tagtgccagt
    52381 ctggctgaaa ccgacgcgta atgcgttggg gattcttggt gggatcccgc gccgtgaatt
    52441 tactcgcgac agcatcgaag agaaagtcgc tgccaccacg caggcacaat ggccggttca
    52501 tgcggtgatt accaactcca cctatgatgg cttgctctac aacaccgact ggatcaaaca
    52561 gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtaca cccattttca
    52621 tccgatctac caggggaaaa gtggtatgag cggcgagcgt gttgcgggaa aagtgatctt
    52681 cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc tgatccacat
    52741 taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata ccaccacctc
    52801 gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc gtggtaatcc
    52861 gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca aagaggtcca
    52921 gcggttgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga
    52981 tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg cacggcttta acgatgcgga
    53041 tgccgatcat atgtttctcg atccggttaa agtcactatt ttgacaccgg ggatggacga
    53101 acagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcaaaat tcctcgacga
    53161 acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct ttagtattgg
    53221 tatcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta aacgctctta
    53281 cgatctcaac ctgcggatca aaaatatgct acccgatctc tatgctgaag atcccgattt
    53341 ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca
    53401 cgatcttccc ggtttgatgt tgcgggcatt cgatactttg ccggaaatga tcatgacgcc
    53461 acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact
    53521 ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat
    53581 gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac tgatgctttg
    53641 ttccgtagtg caacattacc ccggttttga aacggatatt cacggcgcga aacaggacga
    53701 agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggcttc
    53761 cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt
    53821 caccatattg cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt
    53881 cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat
    53941 ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc gccggagcgc
    54001 cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga tgacatcgat
    54061 gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca
    54121 tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt ggaactgtat
    54181 gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat tgctactgta
    54241 aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac gccagattct
    54301 ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg
    54361 gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt taagtgccaa
    54421 tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt
    54481 ggtcgaacgc gtgcaacttg cgcaagaagg actgggcatt gaggcccagg cgcggcaggc
    54541 gcgttatcag gcatttgccc gcaccttgtt gcccggtgaa gtgctggtca ccgcgcaaca
    54601 tctcgacgat caatgtgaaa cttttctgct ggcgctaaaa cgcggtagcg gccctgccgg
    54661 gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc gcccgttgct
    54721 cgcccgcacg cggggagaac tggagcagtg ggcattggcg catggtttac gctggattga
    54781 agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc gcgtagtgcc
    54841 gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg ccgcactttg
    54901 tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac actgccaaac
    54961 gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc gccgcgcggc
    55021 gattatccgt cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg acgcgctggt
    55081 gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt tacgcttggg
    55141 cgcgtttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg tcaccgggca
    55201 aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg ctggaattac cggcggggct
    55261 gggaagcgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt
    55321 cagcgtgcgt ttcaaagcgg caggattgct gcatattgtc gggcgcaacg gcggacgtaa
    55381 gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca ccacgccact
    55441 gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc aagaaggtgt
    55501 ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt aagtgaaagc
    55561 cggataagac gcatcaaacg tcgcatccgg cgaaagttaa tcaggactcg ctcaccacca
    55621 ccgtaccgat ttccgggtgg ctaaagctgg taattttatc cagacgtagc tcacgggttt
    55681 cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa tcgctggctt
    55741 ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga tgctggcagg
    55801 cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca ttcattgaca
    55861 taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac agcctcagaa
    55921 agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag cgcgtccgga
    55981 acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa ctcacaatat
    56041 ttttccacat gccctcctgt cagcactctg acttaaccgt ggatgcaagt ctaagcctac
    56101 gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa tatcagcgcc
    56161 gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt cttatggcgc
    56221 ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc aaccagtttc
    56281 tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt atgatgctgc
    56341 gcttgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt ggtttgtgtg
    56401 ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat ttgtggattg
    56461 agctggggct gccggatgag cggcggatta agaaagcctg cacccaggcc gcagaagtgg
    56521 cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat cagagcaaat
    56581 gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg gcgaaagtaa
    56641 gcgtctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggc gtgatctggt
    56701 tatcggacga taagaataat ctggaagtta acttaaccgc ctggcaacaa ccttcatgat
    56761 tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg ccattcgtgc
    56821 gcagggcgcg ggcgggcagc atgttaataa gacctcaacg gccattcatc tgcgttttga
    56881 cattcgggcg tccagcctgc cagagtatta caaagagcgt ctactcgccg ccagccatca
    56941 tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca gtcaggaact
    57001 gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt aaagatttaa caacagaaca
    57061 aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc tggcatcgaa
    57121 agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc gggaataaaa
    57181 agaaggaata gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc agcctcttta
    57241 ctctgatggg atgtaataac cgggccgaag tcgatacgct ttctccagca caggctgccg
    57301 aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat tgcgaaggaa
    57361 tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag cgttatctcg
    57421 gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga accgctgaca
    57481 agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa ggcgatgcgc
    57541 tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat acgctggaac
    57601 cggcacaatc tagtttacct atgacgccga tgaccctgcg aggcatgtat ttttatatgg
    57661 ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta gcgaataacg
    57721 cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg gtgttactgt
    57781 cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca accaaagtat
    57841 tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt ttggggcagt
    57901 aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct tatcaggcct
    57961 acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata aggcgtttac
    58021 gccgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc caacaaagcg
    58081 acccgccttg atataaatcc caggcttcag gtgcccaaca tactgtaact gcgtttctgg
    58141 cttaaacgca gagacatcac caatacgcat atgcatcaga tcggcagggc taatccagcc
    58201 cgattgcgcc agtgtaagtg gatgcccggc tttggcaaag gcatccgtga caaattccga
    58261 acaaaaccac gactttttgt ctccttcgcc cacactgctt aactgcgctt tcgccaggcc
    58321 gctgacgcac tttggcgaaa atcctcggag aacggattca gtgagcacat ctggcgagtc
    58381 accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc tttgatttta
    58441 ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg
    58501 aaaagcttat cactatgctt catcgctttt ttaagggaaa caatctggac gccagcgcct
    58561 gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac agaggaagtg
    58621 ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag caaatcgccg
    58681 ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc
    58741 tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga tgggtctggc
    58801 tggctgatat caacggtaca agccgaaagt aaaagaaagc tggggagaaa caggcggcag
    58861 tacgcctttg gtttatccat tttatacaat ccatgtaaaa aaagggccct gaaattcagg
    58921 accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg tcaccagtct
    58981 taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg ttgtcgaggt
    59041 tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca gcaaacatca
    59101 cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca cgcagttcgc
    59161 tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc atcggcagaa
    59221 tcgccacctg gaacggcgcg atagcgtcag gccatacgat gcctcgttcg tcgtagttct
    59281 gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc atcgtcagga
    59341 tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag tacttggtac
    59401 ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta ccctggccat
    59461 ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg gtagcgacat
    59521 cgcgatccca gttgatgccg aagtagtgtt taccatcgat gttagcacca gcagcgaaat
    59581 cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt accggaccca
    59641 gtgaacccgg accggcttta accacggcac gaatttcttc ttcggtcgcg aaagtcagcg
    59701 ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga tcaccgcgca
    59761 ccagcagcgc aaccagcggg aagctactgc cttcaaccgc tttaaccagc agagtcttaa
    59821 ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt ttcgcgttcg
    59881 gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc ggcgcgatag
    59941 cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc acatcgtctt
    60001 caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata gaaccggtgt
    60061 cggcttgtac ggcgcggaaa tccagcccca tgcggctgaa gattttgctg taggccgcat
    60121 acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag taagcatctt
    60181 tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg tcgcggaact
    60241 tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc tcgttacgaa
    60301 tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc tcgccacggt
    60361 caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct tgccacaaat
    60421 cagctggctg aaccaccggc atcgacacct cgatcgcacc ggcgttgttc atctcttcac
    60481 gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag gtatataacc
    60541 cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg atcacctcgg
    60601 cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc atgttgttac
    60661 ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa aagtgattta
    60721 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60781 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60841 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgtggg tcctgcgcca
    60901 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    60961 tttcgacctc tgcggtaaaa ctcaccgcca tctctgccgc tggcgcactt tgcgcatagc
    61021 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    61081 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    61141 caacctcttt cagctctacc agcgacatac caattgggtt agggcggaaa gtagagcgtg
    61201 ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc
    61261 agccgccttc catcgtttga tgaaagacga aaaggatcca taaatggctg aacgcttcca
    61321 ggccgcgaac ggcgtcggcc tggttgtagg gagcaatgag atgcagttcg ccgttggcgc
    61381 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61441 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61501 acatacagcc tgacgatagc agcctggcgt accgctggtg acttcgcagc tatgcagtaa
    61561 taccgcattg gccttcattt tcgaggcgtt gatttgcatc cgcttacgcg cggttggaat
    61621 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61681 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61741 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61801 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61861 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    61921 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    61981 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    62041 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    62101 ttcacgcgtt acgatcaggt ttacgtacgg ggagtcttta tcttcgacaa agataccgtc
    62161 tttagctgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    62221 ttgcgcgtcg tccagagatc gaggcagctg cggtgcttcc agctcaacaa ttttcagatt
    62281 ttttgggttc tcaacaacat caagaacggt cggcagcagg ccaacgccat ctttcagttt
    62341 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62401 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62461 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62521 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62581 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62641 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62701 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62761 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62821 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    62881 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    62941 cgcgttatag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    63001 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    63061 cagacccggc aatgcttccg gtaacagcac tttacggacg atctgcatcg gcgtggcacc
    63121 cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat
    63181 acgggcaata aacggtgctg caccaacggt taacggaaca atcgctgcct gcaaaccaat
    63241 cgatgtacca acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63301 gatagaacgg aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat
    63361 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaagcc
    63421 aaaaaaaccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63481 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63541 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaact
    63601 taacgccacc ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63661 gcgcggtttc agaaagcagt ggggcatcga ccgactgacc ggtaaactcc agacgcagca
    63721 tcggcacgca gtcagtgaat ggctccgctt gcagacgttc ctggtaatct tccgggatat
    63781 ccagatgcag ggtcgactga ataaacttct gcgccaacgg cgttttcgga tgcgaaaaca
    63841 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    63901 aaatacgctt tacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc
    63961 ggttaatgtc tttcagcagc tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    64021 tggcttcatc acacagcaat actttgggat tgctggctaa cgcacgggca atcgccacac
    64081 gctgtttctg cccaccggaa agattcgatg ggtagctatc atgcttatcg ccaagaccaa
    64141 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    64201 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64261 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcagtt
    64321 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64381 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64441 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64501 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatc
    64561 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64621 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64681 tcacatgcct tattaaggag ctataaaagg tggcaaagag cgtacccgca atttttcttg
    64741 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64801 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64861 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    64921 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    64981 attgcccgca tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gactgccgta
    65041 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    65101 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggctgcggcg aacgtgggaa
    65161 caaaagtgct ggtgcgtacg ggtaaaccta ttacgcctga agcagaaaac gcggcggatt
    65221 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcat
    65281 aatgattaaa agatgagcaa ttgaaataaa aatgcatttt tccacttgtc ttcctgagac
//
LOCUS       DAZGCS010000013        61053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGCS010000013 DAZGCS010000000
VERSION     DAZGCS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.013"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..1187)
                     /gene="hemW"
                     /locus_tag="ACHK01_000882"
     CDS             complement(51..1187)
                     /gene="hemW"
                     /locus_tag="ACHK01_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO8095877.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(1180..1773)
                     /gene="rdgB"
                     /locus_tag="ACHK01_000883"
     CDS             complement(1180..1773)
                     /gene="rdgB"
                     /locus_tag="ACHK01_000883"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO8095878.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(1781..2071)
                     /gene="yggU"
                     /locus_tag="ACHK01_000884"
     CDS             complement(1781..2071)
                     /gene="yggU"
                     /locus_tag="ACHK01_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO8095879.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(2068..2634)
                     /gene="yggT"
                     /locus_tag="ACHK01_000885"
     CDS             complement(2068..2634)
                     /gene="yggT"
                     /locus_tag="ACHK01_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO8095880.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(2652..3356)
                     /gene="yggS"
                     /locus_tag="ACHK01_000886"
     CDS             complement(2652..3356)
                     /gene="yggS"
                     /locus_tag="ACHK01_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO8095881.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3374..4354
                     /gene="yggR"
                     /locus_tag="ACHK01_000887"
     CDS             3374..4354
                     /gene="yggR"
                     /locus_tag="ACHK01_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO8095882.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(4543..4959)
                     /gene="ruvX"
                     /locus_tag="ACHK01_000888"
     CDS             complement(4543..4959)
                     /gene="ruvX"
                     /locus_tag="ACHK01_000888"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO8095883.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(4959..5522)
                     /gene="yqgE"
                     /locus_tag="ACHK01_000889"
     CDS             complement(4959..5522)
                     /gene="yqgE"
                     /locus_tag="ACHK01_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO8095884.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(5631..6581)
                     /gene="gshB"
                     /locus_tag="ACHK01_000890"
     CDS             complement(5631..6581)
                     /gene="gshB"
                     /locus_tag="ACHK01_000890"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO8095885.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(6594..7325)
                     /gene="rsmE"
                     /locus_tag="ACHK01_000891"
     CDS             complement(6594..7325)
                     /gene="rsmE"
                     /locus_tag="ACHK01_000891"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO8095886.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(7405..8112)
                     /gene="endA"
                     /locus_tag="ACHK01_000892"
     CDS             complement(7405..8112)
                     /gene="endA"
                     /locus_tag="ACHK01_000892"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO8095887.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(8207..8704)
                     /gene="yggI"
                     /locus_tag="ACHK01_000893"
     CDS             complement(8207..8704)
                     /gene="yggI"
                     /locus_tag="ACHK01_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO8095888.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(8781..10175)
                     /gene="galP"
                     /locus_tag="ACHK01_000894"
     CDS             complement(8781..10175)
                     /gene="galP"
                     /locus_tag="ACHK01_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO8095889.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(10611..11765)
                     /gene="metK"
                     /locus_tag="ACHK01_000895"
     CDS             complement(10611..11765)
                     /gene="metK"
                     /locus_tag="ACHK01_000895"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO8095890.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            11821..12072
                     /gene="yqgD"
                     /locus_tag="ACHK01_000896"
     CDS             11821..12072
                     /gene="yqgD"
                     /locus_tag="ACHK01_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO8095891.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            12183..12326
                     /gene="yqgG"
                     /locus_tag="ACHK01_000897"
     CDS             12183..12326
                     /gene="yqgG"
                     /locus_tag="ACHK01_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO8095892.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            12420..12551
                     /gene="yqgB"
                     /locus_tag="ACHK01_000898"
     CDS             12420..12551
                     /gene="yqgB"
                     /locus_tag="ACHK01_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO8095893.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            12560..14536
                     /gene="speA"
                     /locus_tag="ACHK01_000899"
     CDS             12560..14536
                     /gene="speA"
                     /locus_tag="ACHK01_000899"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO8095894.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            14673..15593
                     /gene="speB"
                     /locus_tag="ACHK01_000900"
     CDS             14673..15593
                     /gene="speB"
                     /locus_tag="ACHK01_000900"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO8095895.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            15744..17306
                     /locus_tag="ACHK01_000901"
     CDS             15744..17306
                     /locus_tag="ACHK01_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8095896.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRALCGPEFVTSTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSYIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQHRDFPLLTRTAPERVSNSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLVAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YAEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTETAFAWACGVSTADVVEVQ
                     PDTPDKAIYEAN"
     gene            complement(17392..18150)
                     /gene="loiP"
                     /locus_tag="ACHK01_000902"
                     /gene_synonym="yggG"
     CDS             complement(17392..18150)
                     /gene="loiP"
                     /locus_tag="ACHK01_000902"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO8095897.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            18428..20419
                     /gene="tkt"
                     /locus_tag="ACHK01_000903"
     CDS             18428..20419
                     /gene="tkt"
                     /locus_tag="ACHK01_000903"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8095898.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            20733..21176
                     /gene="cmtB"
                     /locus_tag="ACHK01_000904"
     CDS             20733..21176
                     /gene="cmtB"
                     /locus_tag="ACHK01_000904"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO8095899.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAVDADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            21204..22592
                     /gene="cmtA"
                     /locus_tag="ACHK01_000905"
     CDS             21204..22592
                     /gene="cmtA"
                     /locus_tag="ACHK01_000905"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO8095900.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNLGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            22607..23884
                     /gene="yggP"
                     /locus_tag="ACHK01_000906"
     CDS             22607..23884
                     /gene="yggP"
                     /locus_tag="ACHK01_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8095901.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            23881..24846
                     /gene="glpX"
                     /locus_tag="ACHK01_000907"
     CDS             23881..24846
                     /gene="glpX"
                     /locus_tag="ACHK01_000907"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8095902.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDTYMKKLVVNRLAAGAIDLSLPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            24868..25377
                     /gene="fumE"
                     /locus_tag="ACHK01_000908"
     CDS             24868..25377
                     /gene="fumE"
                     /locus_tag="ACHK01_000908"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO8095903.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            25374..26087
                     /locus_tag="ACHK01_000909"
     CDS             25374..26087
                     /locus_tag="ACHK01_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO8095904.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(26059..26729)
                     /locus_tag="ACHK01_000910"
                     /pseudo
     CDS             complement(26059..26729)
                     /locus_tag="ACHK01_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(26723..27400)
                     /locus_tag="ACHK01_000911"
     CDS             complement(26723..27400)
                     /locus_tag="ACHK01_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8095905.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(27388..28095)
                     /locus_tag="ACHK01_000912"
     CDS             complement(27388..28095)
                     /locus_tag="ACHK01_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO8095906.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(28096..28674)
                     /locus_tag="ACHK01_000913"
     CDS             complement(28096..28674)
                     /locus_tag="ACHK01_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO8095907.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(28697..29128)
                     /locus_tag="ACHK01_000914"
     CDS             complement(28697..29128)
                     /locus_tag="ACHK01_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO8095908.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            29500..30519
                     /gene="epd"
                     /locus_tag="ACHK01_000915"
     CDS             29500..30519
                     /gene="epd"
                     /locus_tag="ACHK01_000915"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO8095909.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            30569..31732
                     /gene="pgk"
                     /locus_tag="ACHK01_000916"
     CDS             30569..31732
                     /gene="pgk"
                     /locus_tag="ACHK01_000916"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO8095910.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            31947..33026
                     /gene="fbaA"
                     /locus_tag="ACHK01_000917"
     CDS             31947..33026
                     /gene="fbaA"
                     /locus_tag="ACHK01_000917"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8095911.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(33090..33188)
                     /locus_tag="ACHK01_000918"
                     /pseudo
     CDS             complement(33090..33188)
                     /locus_tag="ACHK01_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33384..34244
                     /gene="mscS"
                     /locus_tag="ACHK01_000919"
     CDS             33384..34244
                     /gene="mscS"
                     /locus_tag="ACHK01_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8095912.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            34383..35018
                     /gene="argO"
                     /locus_tag="ACHK01_000920"
     CDS             34383..35018
                     /gene="argO"
                     /locus_tag="ACHK01_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO8095913.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            35111..35851
                     /gene="yggE"
                     /locus_tag="ACHK01_000921"
     CDS             35111..35851
                     /gene="yggE"
                     /locus_tag="ACHK01_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO8095914.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVSDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            36018..36914
                     /gene="ygfI"
                     /locus_tag="ACHK01_000922"
     CDS             36018..36914
                     /gene="ygfI"
                     /locus_tag="ACHK01_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8095915.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMSASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(36911..38389)
                     /gene="scpC"
                     /locus_tag="ACHK01_000923"
     CDS             complement(36911..38389)
                     /gene="scpC"
                     /locus_tag="ACHK01_000923"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO8095916.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(38413..39198)
                     /gene="scpB"
                     /locus_tag="ACHK01_000924"
     CDS             complement(38413..39198)
                     /gene="scpB"
                     /locus_tag="ACHK01_000924"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO8095917.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHAIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(39209..40204)
                     /gene="meaB"
                     /locus_tag="ACHK01_000925"
     CDS             complement(39209..40204)
                     /gene="meaB"
                     /locus_tag="ACHK01_000925"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO8095918.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(40197..42341)
                     /gene="scpA"
                     /locus_tag="ACHK01_000926"
     CDS             complement(40197..42341)
                     /gene="scpA"
                     /locus_tag="ACHK01_000926"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO8095919.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRIVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(42545..43438)
                     /gene="argP"
                     /locus_tag="ACHK01_000927"
     CDS             complement(42545..43438)
                     /gene="argP"
                     /locus_tag="ACHK01_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO8095920.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            43580..43765
                     /locus_tag="ACHK01_000928"
     CDS             43580..43765
                     /locus_tag="ACHK01_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095921.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            43821..44480
                     /gene="rpiA"
                     /locus_tag="ACHK01_000929"
     CDS             43821..44480
                     /gene="rpiA"
                     /locus_tag="ACHK01_000929"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO8095922.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAYFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            44736..45968
                     /gene="serA"
                     /locus_tag="ACHK01_000930"
     CDS             44736..45968
                     /gene="serA"
                     /locus_tag="ACHK01_000930"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO8095923.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            46197..46256
                     /gene="ibsC"
                     /locus_tag="ACHK01_000931"
     CDS             46197..46256
                     /gene="ibsC"
                     /locus_tag="ACHK01_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO8095924.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            46589..46648
                     /locus_tag="ACHK01_000932"
     CDS             46589..46648
                     /locus_tag="ACHK01_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8095925.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(46749..47351)
                     /gene="fau"
                     /locus_tag="ACHK01_000933"
     CDS             complement(46749..47351)
                     /gene="fau"
                     /locus_tag="ACHK01_000933"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO8095926.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(47372..47555)
                     /gene="ssrS"
                     /locus_tag="ACHK01_000934"
     ncRNA           complement(47372..47555)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK01_000934"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(47597..47926)
                     /gene="zapA"
                     /locus_tag="ACHK01_000935"
     CDS             complement(47597..47926)
                     /gene="zapA"
                     /locus_tag="ACHK01_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8095927.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            48094..48672
                     /gene="ygfB"
                     /locus_tag="ACHK01_000936"
     CDS             48094..48672
                     /gene="ygfB"
                     /locus_tag="ACHK01_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO8095928.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            48698..50023
                     /gene="pepP"
                     /locus_tag="ACHK01_000937"
     CDS             48698..50023
                     /gene="pepP"
                     /locus_tag="ACHK01_000937"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO8095929.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            50020..51198
                     /gene="ubiH"
                     /locus_tag="ACHK01_000938"
                     /gene_synonym="visB"
     CDS             50020..51198
                     /gene="ubiH"
                     /locus_tag="ACHK01_000938"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO8095930.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGETLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            51221..52423
                     /gene="ubiI"
                     /locus_tag="ACHK01_000939"
                     /gene_synonym="visC"
     CDS             51221..52423
                     /gene="ubiI"
                     /locus_tag="ACHK01_000939"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO8095931.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLVADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(52497..52580)
                     /locus_tag="ACHK01_000940"
     CDS             complement(52497..52580)
                     /locus_tag="ACHK01_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8095932.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            52870..53964
                     /gene="gcvT"
                     /locus_tag="ACHK01_000941"
     CDS             52870..53964
                     /gene="gcvT"
                     /locus_tag="ACHK01_000941"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO8095933.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            53988..54377
                     /gene="gcvH"
                     /locus_tag="ACHK01_000942"
     CDS             53988..54377
                     /gene="gcvH"
                     /locus_tag="ACHK01_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO8095934.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTFTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            54496..57369
                     /gene="gcvP"
                     /locus_tag="ACHK01_000943"
     CDS             54496..57369
                     /gene="gcvP"
                     /locus_tag="ACHK01_000943"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO8095935.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            57630..58373
                     /gene="ygfF"
                     /locus_tag="ACHK01_000944"
     CDS             57630..58373
                     /gene="ygfF"
                     /locus_tag="ACHK01_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8095936.1"
                     /translation="MVIELVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(58430..59863)
                     /gene="bglA"
                     /locus_tag="ACHK01_000945"
     CDS             complement(58430..59863)
                     /gene="bglA"
                     /locus_tag="ACHK01_000945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO8095937.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            59908..60219
                     /gene="yqfB"
                     /locus_tag="ACHK01_000946"
     CDS             59908..60219
                     /gene="yqfB"
                     /locus_tag="ACHK01_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO8095938.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            60383..61042
                     /gene="yqfA"
                     /locus_tag="ACHK01_000947"
     CDS             60383..61042
                     /gene="yqfA"
                     /locus_tag="ACHK01_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO8095939.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT        14560 a        15337 c        16336 g        14820 t
ORIGIN      
        1 cgggggaggg ggggaagaag tgattaatgc gcccgcagac gcattaatga ttactcagcg
       61 aggaataatt caagaagtga gtttaagaat aatttaccat gttccgttat ctgccagtaa
      121 tccgcacatt cggtgagata accctgggca atcgcctcgt ctaactgtgg gcgaatcaca
      181 tcttcgcaaa gcccggtata cgcaataaac tccacgcgcg gcgcggcctc cagcagacgg
      241 aagcgattca taaagaactc aaacggctta tctgcggctt cgacatcacg ctggctttcc
      301 agataccttc cttgcataaa accacgcgga tgacgcgttt tagtggtacg cagaatgcgc
      361 ccatccggga aggtcacttt gccgtgcgcg ccgcagccaa taccgatgta gtcgccaaag
      421 cgccagtagt tgagattgtg ctggcactga taactgggtt tggcgtaagc ggaagtttca
      481 tattgctgat aacccgctgc ggttaataac tgatgcccct gttcgaatat atcccacagc
      541 gcgtcgtcgt ccggcagtac cggcgggcgt gaaccaaaca gcgtattagg ttcgatggtc
      601 agttgatacc aggaaagatg cggcggattc agttcaatgg cctggcgtag atcgccaagc
      661 gcctcttcca gtgattgatc cggtagtcca tgcatcaaat cgaggttaaa gctacgcagc
      721 cccagcccgc tcgccagctt tgccgcgcgt ttcgcttctt gcgggccatg aatacgccca
      781 agtcgtttca gcttgtcttc gctaaaactc tgcacaccaa tagagatgcg gttcacacca
      841 gcacgctgat aatcgacaaa gcgatcggct tctaccgtac cagggttcgc ttccatagta
      901 atttctgcat ccgctgccag cggcaaacgc gcacgcacgc cgtccagcag cgtttgcatc
      961 gccgggccgg aaagcaggct cggcgtacca ccgccaataa agattgtctt tacttcacgg
     1021 ccctgagcgt aagccacatc gttgtccaga tcgcaaagca gatgctgaac gtaatcgtcg
     1081 tgcggcactt ctcctttcaa cgcgtgagag ttgaaatcgc agtacgggca tttctgcacg
     1141 caccacggga tgtgaatgta gagactcagc ggcggtaatt taaccattac gtaaagcgtc
     1201 caacagcagt ttcaatgcct gaccacggtg ggaaatggcg ctcttttctt cgcgggtcag
     1261 ttcggcagcg gttttccctt cggaaggtac gaagaagatt ggatcataac caaagccacc
     1321 agtgcccgct ggttcacgag taatcacgcc cggccagcta ccgtggcaca ccagcggagt
     1381 cggatcttcc gcgtgacgca gatataccag cacgcagtgg aaacgcgcct gacgttggtc
     1441 gtccggtacg tctttcagtg tttccaacag tttttgcaga ttcttttgat cggtcgcgtc
     1501 ttcaccggaa taacgcgcgg agtaaatacc cggcgcgcca cccagcgcat ctaccgccag
     1561 accagagtcg tcggcaattg ccggtaaacc ggtcactttt gccgcatggc gcgctttcag
     1621 aatcgcgttt tcgataaagg tcaggccggt ttcttcagcg gaatctacgc cgaggtctgt
     1681 ttgggccacg atatcaagac cgaagtcgct aagcagcgac gccagctcac gcactttacc
     1741 ggcattgccg gttgcgagga caactttttg cataggatac ctaattaatt aacgccgcga
     1801 tttctggcgg gatttgttgc ggattaatga ttttaatttg tttgtggcgg ccaagttcgc
     1861 ctttttcaat taccacctgg cttttggcta cccggaattg cttaccgaga aacttcacca
     1921 gatggctgtt ggcctggccg tcaaccggcg gcgcggtaat ggcgacttta acttcgtcgc
     1981 catgtaaacc gacaatagaa tcacggctgg ctttcggctg aatatagagc cgtaaaacca
     2041 gaccgtcatc attaactgtt acggcactca taacgccatc cacagccccg gcagcagcat
     2101 gtttccggtt gcctgtaata cttctgcgac acccatattg atgacataca gcagcagaac
     2161 gaggatcatc ggcgagaaat caatcccacc cattgccggt agcaggcggc gaatcgggcg
     2221 cagcagcgga tcggccagct gaatcagcac gtattcaatc ggactacgac cctggcttac
     2281 ccagctcata atcgccatca ccagcaggac ccagaaaatc agcaggccga tggttttcag
     2341 cagaatcagt aaaccggcaa tccagatgat tggcaggaag gtcaccactt taaacagcac
     2401 gatggctttg ataaaactga gaatgtacgc aaccagcagc gaggcgctat caatgggacc
     2461 cattgccgga ataacgcggc gcagtggccc gataattggc tgtgttacct tcactacaaa
     2521 ctgtgagaag gggttgtaaa aatcacaatg agcccactgc atccagatgc gtaataacag
     2581 caccatggta tacagctcaa tgaccgttga aagcaggaaa gtcaacgtat tcatggcgtt
     2641 cctttaattc cttatttttt agagtaatca cgcgcaccaa aaattgcagt gccgatacga
     2701 accatcgtgc tacctgccgc aatggcggct tccatatcgt ccgacattcc cagagagagc
     2761 gtgtcgatat gcgggtagcg cgttttcagt ccggcaaatg ctacagccat ttggcgtgca
     2821 acttcaaact gccttacata ttctgactca ggcgcaggga ttgccatcag cccgcgcaga
     2881 cgtaaacgcg gtagttcagc gaccgccgct gccagctcgt ccagttctgc cagttgaatc
     2941 ccggacttac tgttttcatc actaatatta atttgaatca gtacgttaag agggggaagt
     3001 tctgccgggc gctgatcgtt gaggcgggta gcaatgcgca aacggtcgat ggtatgacac
     3061 cagtcgaaat gctctgccac caggcggctt ttattagact gcaacgggcc aataaaatgc
     3121 cattctaatc ctgttacgcc cagttcctga aagtggcgaa ttttatctac cccttcctga
     3181 acgtagtttt caccaaattg acgctgcccg gcatcaatgg cttctgcgat ggcgctcgca
     3241 ggttttgttt tactgactgc aagcagcgta atttcttctg gagaacggcc gcaacgcgtt
     3301 gcagccgctg agattttgtc ccggacctgt gccaggttat gcgcaatatc gttcattttc
     3361 cgaggatctt agtatgaata tggaagaaat tgtggccctt agtgtaaagc ataacgtctc
     3421 ggatctacac ctgtgcagcg cctggcccgc acgatggcgc attcgtggca gaatggaagc
     3481 tgcgccgttt gatgcgccgg acgtcgaaga gctactgcgg gagtggctgg atgacgatca
     3541 gcgggcaata ttgctggaaa atggccagct ggattttgcc gtgtcgctgg cggaaaacca
     3601 gcggttgcgt ggcagtgcgt tcgcgcaacg gcaaggtatt tctctggcat tacggttgtt
     3661 accttcgcac tgtccacagc tcgaacagct tggcgcacca acggtattgc cggaattact
     3721 caagagcgag aatggcctga ttctggtgac gggggcgacg gggagcggca aatctaccac
     3781 gctggcggcg atggttggct atctcaatca acatgccgat gcgcatattc tgacgctgga
     3841 agatcctgtt gaatatatct atgccagcca gcgatgtttg atccagcagc gggaaattgg
     3901 tttgcactgt atgacgttcg catcgggatt gcgggccgca ttgcgggaag atcctgatgt
     3961 gattttgctc ggagagctgc gtgacagcga gacaatccgt ctggcactga cggcggcaga
     4021 aaccgggcat ttggtgctgg caacattaca tacgcgtggt gccgcgcagg cagttgagcg
     4081 actggtggat tcatttccgg cgcaggaaaa agatcccgta cgtaatcaac tggcaggtag
     4141 tttacgggcc gtgttgtcac aaaagctgga agtggataaa caggaaggac gcgtggcgct
     4201 gtttgaatta ttgattaaca ctcccgcggt ggggaatttg attcgcgaag ggaaaaccca
     4261 ccagttaccg catgttattc aaaccgggca gcaggtgggg atgataacgt ttcagcagag
     4321 ttatcagcag cgggtggggg aagggcgttt gtgacgttgc catgcccgat gcgcaagttt
     4381 atagggccta taaaggcaat aaatgtctga tgcgacgcta acgcgtctta tcagacctac
     4441 aatcgctgcc acatgtaggc cggataaggc gttcacgccg catccggcaa taaggcgtaa
     4501 ggcgcgatga tattatacac tccagcaggc gtttaaatcg ccttaatatc cctgctcgaa
     4561 atagctttcg agaataataa ccgcagaggc agagtcaact ttgcctttgt tgagcgcccg
     4621 atagccgccc tgttcaaaca gaccggaacg ggcttccacg gtgctaagac gttcgtcatg
     4681 gagctttact tcaacaccga aacgaccatg aatacggttg gcaaatttac gcgctctggc
     4741 agtcaatggt tgctcggtgc cgtccatatt cagcggcaaa ccgacgatga tttcgtccgg
     4801 ctgccactct ttcagtaaac gttcgatgag gttccagtcc ggcgtaccgt cctgtgcttt
     4861 aattgcaggc aaagggcgag cagtgccggt aatgcgttgg ccgaccgcta cgccaatgct
     4921 tttggtgccg aagtcgaagg cgagtaaggt tccactcatc aggcgtgccc tgccacacca
     4981 ggcatggtga gaatatccac accaatcagt tttgccgcct cgcgccagcg gtcggcaatc
     5041 ggtgttttga acagaatatt cagatccgcc ggggccgtta gccacgcgtt atcgagaatt
     5101 tcttgttcca gttgaccttt ttcccaggag gcataaccca gagccaccaa tacgtcagac
     5161 ggttgtttat cggtgccgag cgtttccagc acatcgcggg aagtggtcat cactgtgttg
     5221 tctgaaatgc gaatgctgga agcaaaattg gagggcggag tatgcaaaat aaacccgcga
     5281 tcttcagcca gcggaccgcc gagcataacc ggtttatcca gacggattga ttcatcacgc
     5341 ggctccggcg tgatcttcag cttttccaga atcccttcaa ttttgagatt ttccagcggc
     5401 ttgttgacga tgatccccat tgcaccattg gtattatgtt cgcaaatgta gaccacggaa
     5461 cgacggaaaa tcggatcctg gagagcaggc atggcaataa gaaagtgatg ctgtaaattc
     5521 attgtcagag gttctgtttc ctggttcaaa aaagcaacag cgcccagtat gcgtggaaag
     5581 aagggcgctg tcactcagag tctcaacgag atccttctcg ctaaggtggg ttactgctgc
     5641 tgtaaacgtg cttcgatggc atccattaac attccggtga tcgacaccgg aaactctgct
     5701 tcaatctcac gaatacaggt tgggctggtg acgttaattt cagtcagacg gtcgccgatg
     5761 atatccagac caacaaaaat cagccctttt tctttcagcg tcggcccgat ctgacgggcg
     5821 attttccagt cactttccgt cagcggacga ggttcaccgc gaccaccggc agccagattg
     5881 ccacgggttt cgcccccctg cggaatacgc gccaggcagt acggtaccgg ctcgccatcc
     5941 accaccagca cgcgtttgtc gccatcttta atggctggca ggtaattttg cgccatgcag
     6001 tagcgagtgc catgctcagt cagggtttcg gcaatcacgc cgaggtttgg atcgccttct
     6061 ttcacgcgga aaatcgacgc gccgcccata ccgtccagcg gcttaagaat gatgtcgctg
     6121 tgtttctccc agaacgcttt tagctgcgct ttattgcgcg taaccagcgt ttctggcgtt
     6181 aagtcagaga accaggcggt aaacagtttc tcgttacagt cgcgcaggct ctgcggcttg
     6241 ttaacgatca gcgtcccttt ctcttcggca cgttccagaa tataggtcgc gtagataaac
     6301 tcggtatcaa acggcgggtc tttacgcatc aggatcacat cgagatcggc cagcggcaga
     6361 tcctgttcac cgacgaatga aaaccactct tcgtagttct gcttcacgtt cagcgtgcgg
     6421 gtatgggcgc gggcttcgcc attgatcaga tacagatcgc ccatctccat atagtgaagt
     6481 tcgtaaccac gacgctgtgc ttccagcaac atagcgaaac tggaatcttt cttgatgttg
     6541 atgtttgcga tggggtccat cacgatgccg agcttgatca ttattcttct ccgttagccc
     6601 aaatcgccaa atcgtacttg tagcgcggta atggcggtga gcgcagttgt ctctgtacgc
     6661 aaaacgcgag gtcccaacag gatatcagta aattgatagc gggcagtcat ggcaatttca
     6721 tctgccgata aaccgccttc cgggccaatc agcaggcgga cgcgttcaac cggtaaaggc
     6781 aacgtattga tgctgttact ggcgcgcggg tgaagattca gtttcagtcc ttcatcctgc
     6841 tctgcacacc aggcttccag atccatcgct ggacggattt ccggcacccg gttacgacca
     6901 cactgctcac aggcagcaat tgcaatcttc tgccactgct gaagcttctt gttcagacgt
     6961 tcactatcca gtttaacgcc gcagcgctca gaaaaaagtg gcgtaatgag gcttacaccg
     7021 agttcgatcg atttctggat cgtaaattcc attttttcac cacgcgacat cacctgaccg
     7081 aggtgaatat gcagcggaga ttcgcgatcg tcgatcttgc cttccagcac cttcacttcc
     7141 acgctttttt tgctggcgct ggtaatttcg gcgtcaaaga cctggttgct accgtcaaac
     7201 aattgcaacg cctgccccgg ccccatgcgc agtacgcgcc cgatatggtt ggcggcatct
     7261 tcgcaaagcg cgatgtgaga atggctggtc agtggttcag gatgataaat gcgggggata
     7321 cgcatagtta aaaatccgcg tcgtctcccc acgcggttgt acgcgtgggg taggggttaa
     7381 caaaaagaat cccgctagtg taggttagct ctttcgcgcc tggcaagcgc gttgcacata
     7441 cgggttatga ttgccctgca ccttcgcgat gcgttcatcg cgctcgcact cccagtcggt
     7501 aaccggatac atcttgttcc atgcgttgaa cagctgcgtt tgctggcgag agagtgtcag
     7561 gttgtattgg tcgcgcatat agaagtaggt gcgcgcaatg gcaccgcgtg cacgcgctgg
     7621 tggttcggca gctttttctt tgaaatcgac cttcatggcg cattgaccgt actggccttc
     7681 gccgccattc cactggctgt acataaagtt gccgcgatcg ccattcacct cgccgactga
     7741 cggttgcagg ttatgcatat cgctttccat cttgcgatag accggatctt tagcgcagtt
     7801 tttacgtcca ccgtcctgcc agcactggcg ctggtgaccg aactgccagg cgggaacgac
     7861 gtgttcccac tctacgcggc tggcgcggtt ttcatttttg cgcacctgat agccgcacga
     7921 ttgcagatca acaacgcctt ttttgccctg ccagttaatt ttacatccgc aataaaacgt
     7981 accgggcgcg tcagcgtgga cttttaccgc cgcggctttc gcctgagaaa aactattgat
     8041 accttcggcc aaagccgggc cggaaaatgc tgcgctcagt accaccgcag caatagacaa
     8101 ataacggtac atcacttact ccgtgttaaa acgagccagc aacgtagcga aagttgtttt
     8161 ggtatgcaat cagccagatc aggaaagttc ctgatagttc agatggttat ttcgcaacca
     8221 gctgttcacc gcagtgaaca cagcgataga cggcctcgcc acgaactacg cgattatggc
     8281 ggcgtacggt aagctgatgc tcctggcact tgcagcggta ggggaaggtg ttgcgacgca
     8341 cggattgcag ttcgaactga tgcgtacgac gggcgggaac acccagcacg ctttccatca
     8401 tccacttcca ctctttgcca tgtggcgcta cgcggccgaa atgtttccat accagcaaat
     8461 gtgccagttc gtgcggtacc acttcttcaa taaaagcttc actgttttcc aacagcaaaa
     8521 cgggattgag gcgaatttca tagctttcca gccaggccgt tccggcggag gttccgcgct
     8581 gggtgtaaga gagttttggc tccgggtagt tacgccctag cttcaggttg gcctgggcga
     8641 gtttttcccg caggcgacgc ataacggcct gttggatggc gatagggaga cgggatgttt
     8701 tcatagcggc agaggataga gcaaagaaaa gatgactgca agaggtggct tcctccgcga
     8761 cgggaggaag cctgaggaga ttaatcgtga gcgccgattt cgcgcagttt acgacctttc
     8821 atcagattac gttcaatatg ttccagcgaa acgtgtttgg tttccggtac cagccacaat
     8881 gtcagcagga taaacagtac gttcagagcc gcatacaccc agaaggtgtt ggcgttaccc
     8941 agcgtgttga gcatggtcag gaacgttgcg ccaacgatca tgttggcaat ccagttggtg
     9001 gcagtggagc aggtgatgcc aaaatcgcgg cctttcagcg gctgaatttc ggagcacagt
     9061 acccaaatca gcggaccggc actcatggca aaaccgacaa taaacatcag cagcatggcg
     9121 atggcgaaat actgcgccga cggagagtga ataccgatat gcatcattgt accgagtacg
     9181 cccatgccag cagccatcac caggaagccc agcgttagcg ttggtttacg tccccagcgg
     9241 tcaacaaggc cgattgcgat aaaggtggca agtacgttgg tcaggccgac aatcacggtc
     9301 ccccacattt gctcggtagt gttggtataa cccgccagtt cgaagatttt cggcgcgtaa
     9361 tacatgatga cgttcatccc ggtgaattgc tgcattacct gcaacagtac gccaaggaac
     9421 accgcgcggc ggaagttgct gttctcttta aacagcgccc agccactctg tttaacctgc
     9481 aaactttcac ggatttcatc cagttcgcgt ttcgcttccg cgctggtgtc acgcaggcgt
     9541 agcagcacgc gttcggcatc aacaaaacgg cgtttggcgg caaaccaacg tgggctgtct
     9601 ggcaggaaga agacaccaat cagcagcaaa attgccggga tgataatcac acccagcatc
     9661 cagcgccatg caccggtgta gctgaaggcg gtatcagaaa gataagcacc gaggatcccg
     9721 atagtgatca tcaactgata catcgagatc atactgccac gaattttttc cggcgcaatt
     9781 tcagagaggt acagcggtgc ggtataagag gccacaccca ccgccagccc cagtagaacg
     9841 cgggaaagaa tcagtacttc aacgtttggc gcagccgcag agaacagcga accggcaaca
     9901 aacaaaattg cgccgatcat caggctcttt ttgcgcccga gtttaaagga gagccagccg
     9961 ctgcccaccg caccgactgc cgcaccgaac atcatggagc ttacgaccca ttcttgcgtg
    10021 tgcgaagtaa tctggaattc atctgcaata aacggcagtg cgccagcaat tacaccgata
    10081 tccaggccaa agagtaatcc cgccagagcg gcaaggaagc agacgaaaaa cgtcattgcc
    10141 ttgtttgacc gcccctgttt tttagcgtca ggcatgatgc cctccaatat ggttattttt
    10201 tattgtgaat taagataggt gagtacgacg taaaaagatg tgaagcaaat cacatcagtg
    10261 taatcgctta cactaacatt tatcttagtt atggtttaag tgcatgaaaa taattgagtt
    10321 atatcatcat agattaaacg ctgttatctg caattaagac tttactgaaa agaaatgtaa
    10381 caactgtgaa aactgaataa taatatgaaa atattacaaa tatttggctg gtgttattgg
    10441 tgtaatcggt ttcagtagtg atgataattt gcgataaaac gtgttgagga agaatgatgt
    10501 tattgtgcgg gcagcttttt gccggatgcg gcgtgaatgc ctatccaccg cggtgaggta
    10561 aaagaaaaag gccagcgcga ggctggcctt tgaaagcagg tgaagaaaga ttacttcaga
    10621 ccggcagcat cgcgcagcag ctgcgctttg tcggtttttt cccacgggaa atgttcacga
    10681 ccaaagtgac cgtatgctgc ggtttctttg tagatcgggt gcagcagatc cagcatctga
    10741 atcagaccgt atgggcgcag gtcgaagaac tcacgtacca gcagagtcag ttgttcagaa
    10801 ggcactttct cagtaccgaa agtttctacc atgatggagg tcggttcagc cacgccgatt
    10861 gcgtaggaaa cctgaatttc acaacgatcg gccaggccag cagcaacgat gtttttcgcg
    10921 acataacgtg ctgcgtaggc tgcggaacgg tccacttttg atggatcttt accagagaat
    10981 gctccgccac cgtgacgtgc catgccgccg taggtatcaa cgataatttt acgaccagtc
    11041 agaccgcagt cacccatcgg gccaccgata acaaaacgac cggtcgggtt gatgaagaat
    11101 ttggtggcag aagtcagcca ttcagcgggc agaattggct tgatgatctc ttccattacc
    11161 gcttcttgca gcgatttctg gtcgatctct tcagagtgct gagtggaaag cacgacagca
    11221 tcaataccaa cgattttgcc gtcgtcatac tggaaagtca cctggctttt cgcgtccggg
    11281 cgcagccacg gcagagtgcc gtttttacgc acttcagcct gacgctgtac cagacggtgt
    11341 gcataggtga taggtgctgg catcagcacg tcggtttcat tagttgcgta gccaaacatc
    11401 agaccctggt cacccgcgcc ctgttccagc ggatcggcac ggtcaacgcc ctggttgatg
    11461 tcaggagact gtttgccgat agcgctcaga accgcacagg agttagcgtc aaagcccatg
    11521 tcggaatgca catagccaat ttcgcgaacg gtgttacggg tgatctcttc gatgtctacc
    11581 caggcgctgg tggtgatttc gccgccaact aaaaccatgc cggtttttac gtaggtttcg
    11641 caagcaacgc gtgctttcgg atcctgttcg aggatcgcgt ctaaaacggc atcagagatt
    11701 tggtcagcaa ttttgtcagg atgcccttca gagacggact cggacgtaaa aaggtgtttt
    11761 gccatattta atatcaccta aagagaattt ggttagctca aactgttgtg tggattttct
    11821 gtggtagcgg atcctaccac gactctgcaa gttaaaaaca ctggcagtct gagtgttaat
    11881 cggtatggat ggattaacat ctggatggct attttaggtc aattcttcac ccgatttcca
    11941 cttttttttg aatcgtgtct cattctgtta aaaacgtggc tggaaatttt tcctgacaat
    12001 gccggcattc tgcgtattta tcttttgcaa ttttctgcca ttgtggggta taaaacgcgg
    12061 cgcgcggctt aaataaaaag cacacgacgt ttctttcgtg ttgccacttc cagccgggtt
    12121 gaaatcagag ttttggcttg tgggttcgtc ttaacaggcg gccgtggagg tgatacgaaa
    12181 taatgaaccg ttgtctgctg cttaacctgt ctcaccgttc tggtgaagat tcgttccccg
    12241 cactctgcat ctctgctttg catacctgcc gatgttatac ccatctcggc gcttctcagg
    12301 attcaagagc tggttacagt tactgaggac tgaacaaggg cgctcttgta aaaacaagag
    12361 ttttctcgtg gtttcgccga actgtcacac ttacgttcgg ttatgtgctt aataatgtta
    12421 tgaaaaagaa accggttgcg cagttggagc gtcagcattc actgctggaa aatccatgtg
    12481 cttatgggtt gttatcgcag ttccaggctg cgatagtcgt taactgtttt acacttaata
    12541 aaataatttg aggttcgcta tgtctgacga catgtctatg ggtttgcctt cgtcagcggg
    12601 cgaacacggt gtactacgct ccatgcagga ggttgcaatg agctcccagg aagccagcaa
    12661 gatgctgcgt acttacaata ttgcctggtg gggcaataac tactatgacg ttaacgagct
    12721 gggccacatc agcgtgtgcc cggacccgga cgtcccggaa gctcgcgtcg atctcgcgca
    12781 gttagtgaaa actcgtgaag cacagggtca gcgtctgcct gcactgttct gtttcccaca
    12841 gatcctgcag caccgtttgc gttccattaa cgccgcgttc aaacgtgcga gggaatccta
    12901 cggctataac ggcgattact tccttgttta tccgatcaaa gttaaccagc accgtcgcgt
    12961 gattgagtcc ctaattcatt cgggcgaacc gctgggtctg gaagccggtt ccaaagccga
    13021 attgatggca gtgctggcac atgctggcat gacccgtagc gtcatcgtct gcaacggtta
    13081 taaagaccgc gaatatatcc gcctggcatt aattggcgag aagatggggc acaaggtcta
    13141 tctggtcatt gagaagatgt cagaaatcgc cattgtgctg gatgaagcag aacgtctgaa
    13201 tgtcgttcct cgtctgggcg tgcgtgcacg tctggcttcg cagggctcgg gtaaatggca
    13261 gtcctccggc ggggaaaaat cgaagttcgg cctggctgcg actcaggtac tgcaactggt
    13321 tgaaaccctg cgtgaagccg ggcgtctcga cagcctgcaa ctactgcact tccacctcgg
    13381 ttcgcagatg gcgaatattc gcgatatcgc cacaggcgtt cgtgaatcgg cgcgtttcta
    13441 tgtggaactg cacaagctgg gcgtcaatat tcagtgcttc gacgtcggcg gcggtctggg
    13501 cgtggactat gaaggcaccc gttcgcagtc tgactgctcc gtaaactacg gcctcaatga
    13561 atatgccaac aacatcatct gggcgattgg tgatgcgtgt gaagaaaacg gtctgccgca
    13621 tccgacggta atcaccgaat cgggtcgtgc ggtgactgcg catcacaccg tgctggtgtc
    13681 taatatcatc ggcgtggaac gtaacgaata cacggtgccg accgcgcctg cagaagatgc
    13741 gccgcgcgcg ctgcaaagca tgtgggaaac ctggcaggag atgcacgaac cgggaactcg
    13801 ccgttctctg cgtgaatggt tacacgacag tcagatggat ctgcacgaca ttcatatcgg
    13861 ctactcttcc ggcaccttta gcctgcaaga acgtgcatgg gcagaacaac tctatctgag
    13921 catgtgccat gaagtgcaga agcaactgga tccgcaaaac cgtgcgcatc gtccgataat
    13981 cgacgaactg caggaacgta tggcggacaa aatgtacgtc aatttctcgc tgttccagtc
    14041 aatgccggac gcatggggga tcgaccagtt gttcccggtt ctgccgctgg aagggctgga
    14101 tcaagtgccg gaacgtcgcg ctgtgctgct ggatattacc tgtgactctg acggtgctat
    14161 cgaccactat attgatggtg atggtattgc cacgacaatg ccaatgccgg agtacgatcc
    14221 agagaatccg ccgatgctcg gtttctttat ggtcggcgca tatcaggaga tcctcggcaa
    14281 catgcacaac ctgttcggtg ataccgaagc ggttgacgtg ttcgtcttcc ctgacggtag
    14341 tgtagaagtg gaactgtctg acgaaggcga taccgttgcg gatatgctgc aatatgtgca
    14401 gctcgatccg aaaacgctgt taactcagtt ccgcgatcaa gtgaagaaaa ccgatcttga
    14461 tgctgaactg caacaacagt tccttgaaga gttcgaggca ggtttgtacg gttatactta
    14521 tctggaagat gagtaagtcc gtattacttg aatccgctta atttagcggt gataatccgc
    14581 cacaatttat tgtgacaaat ccaacccttc ctcgtcgggc ctaacgacgc ggaagggttt
    14641 ttttatatcg actttgtaat aggagtccat ccatgagcac cttaggtcat caatacgata
    14701 actcactggt ttccaacgcc tttggttttt tacgcctgcc gatgaacttc cagccgtatg
    14761 acagcgatgc tgactgggtg attactggtg tgccgttcga tatggccact tctggtcgtg
    14821 cggggggacg ccacggtccg gcagcgatcc gtcaggtttc gacgaatctt gcctgggaac
    14881 acaatcgctt cccgtggaat ttcgacatgc gtgagcgcct gaacgtcgtg gactgcggcg
    14941 atctggtata tgcctttggc gatgcccgtg agatgagcga aaaactgcag gcacacgccg
    15001 agaagctgct ggctgccggt aagcgtatgc tctctttcgg tggtgaccac tttgttacgc
    15061 tgccgctgct gcgtgctcat gcgaagcatt tcggcaaaat ggcgctggta cactttgacg
    15121 cccacaccga tacctacgcg aacggttgtg aatttgacca cggcaccatg ttctataccg
    15181 cgccgaaaga aggtctgatc gacccgaatc attccgtgca gattggtatt cgtaccgagt
    15241 ttgataaaga caacggtttt accgtgctgg acgcctgcca ggtgaacgat cgcagcgtgg
    15301 atgacgttat cgcccaagtg aaacagattg tgggtgatat gccggtttac ctgacctttg
    15361 atatcgactg cctggatcct gctttcgcgc cgggaaccgg tacgccagtg attggcggcc
    15421 tgacctccga tcgcgccatt aaactggtac gcggcctgaa agatctcaac atcgttggga
    15481 tggacgtagt ggaagtggct ccggcatacg atcagtcgga aatcactgct ctggcagcgg
    15541 caacgctggc gctggaaatg ctgtatattc aggcggcgaa aaagggcgag taagtaacct
    15601 gcctggtcga attttttgca cagtcccttg tctggcacaa gggacttatc atttaaaacc
    15661 tatcctgtta attaagtcat ctttaccctg actgctgttt ttttaatgga ttgttattac
    15721 cgtgttttta atggaataaa tagatggaac gcactcttcc ctggaagcct cttctttgtt
    15781 tgttatttct tttatcaact ggctgcgata gagcagaaaa acagaaaatt gccgcacagc
    15841 tgcaacaaga caaagaagct atttcctctg tcgtcaaaaa gagttatcgc gcattatgtg
    15901 gccctgagtt tgtcaccagc acagattcgc acgatcgcct tgcggaaatg cagcaagtat
    15961 ttacgcctgc tttaatggag cctgtactga aatactggca agagtcaccc aaaacgtgta
    16021 aacgacgttt ccccagttat agcgatcctt tagcgacctt cgtcgaaggc gacaaggcgc
    16081 agattggtgc tttttttggt caccaatctt atattgatat tccaatggaa ttaatccgtg
    16141 aaggggatgg taaatggaag attgcggcga ttgattttgc atcgttacat gagtttgaaa
    16201 aaaacggtcg ggttactgat gaggtgcaga aaaaaataga tttgcttaaa aaacgctcat
    16261 tcaagcaaca tagggatttc cctctgttga cgaggaccgc gccagagcgg gtcagtaatt
    16321 cccctttgac atcggtggag caaacagtta cggagccaga ctcttcctgg ctggatgtct
    16381 tactgacaat aggcaaatat gccctttatc tggtagcgat aatacttatt ctcgccttgg
    16441 tggctgtttt gtggattggt tatggcctgg tgttcgccgg acatcgcctt ttcaaagcat
    16501 cctgtaaaga tccctcaatt caaaaaattc ctgctgccga acgttgggca atggcagtcg
    16561 gcgcgcctta tgccattgcg ggtaacaatc actgggcgcg acgagtcgtt aatgatgatt
    16621 ccgtggaagc agagaatgat tgcaaaagtg aagttgagtc acttgctgac atgtggggcg
    16681 tctttgatcg ggattcgtta cttgagcaac tcttagcttt gtttgtagca gggcaccgtt
    16741 cagtttatgc agaacagatc aaaaacgata gcgaaatgcc ggatgctgag tatcgcgcgt
    16801 tcgctcagca attagctcag aacgcgaaat caagtagcga agcgaaagag cgtttgtggc
    16861 agctacagat ggtcagaaaa aatcgacgta aaatatgcga tatagatttt tgtgcctggg
    16921 atatggtgcg ttttgtcatg ctatgtaata gcggggcgca agtgggatac attacccagc
    16981 gtgaaatggt cgatttttcg atgctggcag ccagacgcgt acagcaacat tacagaagct
    17041 ggcgcgaatt ggccgggcat tttttgctgg cccgttggta ctggaaagca acggataaac
    17101 ggcacctgat tacccatatt ctttttaaga aagccattac atctttatta aaagagcagg
    17161 ggagtccgtg gcgtacttta ccctgggata ctccattacc tgaaggaaca gagacggctt
    17221 ttgcctgggc ttgtggcgta agcactgccg atgtagtaga ggtgcaaccg gatacaccag
    17281 ataaagcaat ctacgaagcg aattgatgcc ccaaatgatt gagcttgttt aatgactctg
    17341 gccggctctg aatgaaaatt tctccgacat tcctgtcaga gaaatatgaa atcactttat
    17401 cccatccgcg ctcatgcgat cgcgaatatg ctgggcgcgt tcggcggatg ccggatggtc
    17461 gtcaaacatt gagctttggc ggccttcttc cagtttcgcc agtttttcaa agctggtggc
    17521 aagacctgcc gggctgatgc cgcgttggcg tagaagatcg taagaataat catcggcttc
    17581 tgcttcctgg cgctgggaga attgcgaatt gactaatttc tcgcccagat caccaagttg
    17641 tgattgagat aaactgccga caatcccgcc cgcagaggcc gcagctactc gcacggcatt
    17701 tgtaccaagc gccacctgca tccctttctt cacatggcct aacgccacgt gccccatctc
    17761 gtgaccgatc accgcttcga cttcgttatc cgtcatcata tccatcagcc cgctatagac
    17821 gcggatacag ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat acactttgta
    17881 attcactggc tgaccgttga tattgttgcc tagcgcattg gcaatagttg tcagacgttt
    17941 agcgtattcg ctattggctg gcgcaatcgt cgccttgctg tccatctcct gacatgcctg
    18001 atcgctcagg gttttcacct gcgcatcact caaactgtaa gcctgaaaag cttccgctcc
    18061 tgatgagagc agtccgttgg agtccatatt ctggcaaccg gtcagtaccg ttgccacgct
    18121 cattgctacc aataaggcgc gaattttcat gttttttctt ccgcacaagg tcaggattat
    18181 tctaaaaatg cgccgtttgc aggtgaaccg acgctcagtc tcagtataag gaaaagcgca
    18241 gcggacgggc gagtagattg cgcaacatgc gagcatgatc cagagatttc tgaagcggca
    18301 aaaggatgtt ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa attttccggc
    18361 gtagcccaaa acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca tccgatctgg
    18421 agtcaaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga gcatggacgc
    18481 agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg acattgccga
    18541 agtcctgtgg cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg ctgaccgtga
    18601 ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc tgcacctcac
    18661 cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact ctaaaactcc
    18721 gggccacccg gaagtaggtt ataccgctgg tgtggaaacc accaccggtc cgctgggtca
    18781 gggtattgcc aacgcagtcg gtatggcgat tgcagaaaaa acgctggcgg cgcagtttaa
    18841 ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg acggctgcat
    18901 gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc tgggtaaact
    18961 gattgcgttc tacgatgaca acggtatctc tattgatggt cacgttgaag gctggttcac
    19021 cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg acatcgacgg
    19081 tcatgacgca gcatccatca aacgcgcagt agaagaagcg cgcgcagtga ctgacaaacc
    19141 gtccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag ccggtactca
    19201 cgactcccac ggtgcgccgc tgggtgatgc agaaatcgct ctgacccgcg aacagctggg
    19261 ctggaaatac gcaccgttcg aaatcccgtc tgaaatctat gcgcagtggg atgcgaaaga
    19321 agcaggccag gcgaaagaat ctgcatggaa tgagaagttt gcggcttacg cgaaagctta
    19381 tccgcaggaa gcggctgaat ttacccgccg tatgaaaggc gaaatgccgt ctgacttcga
    19441 cgccaaagcg aaagagttta tcgctaaact gcaggctaat ccggcgaaaa tcgccagccg
    19501 taaagcgtct cagaatgcta ttgaagcgtt cggcccgctg ttgccggaat tcctcggcgg
    19561 ctccgctgac ctggcaccgt ctaacctgac cctgtggtct ggttctaaag caatcaacga
    19621 agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga ccgcgattgc
    19681 taacggtatc tccctgcacg gtggtttcct gccgtacacc tccaccttcc tgatgttcgt
    19741 ggaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc aggtgatggt
    19801 ttacacccac gactccatcg gtctgggcga agacggcccg actcaccagc cggttgagca
    19861 ggtcgcttct ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg accaggttga
    19921 atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg cactgatcct
    19981 ctcccgtcag aacctggcgc agcaggaacg aactgaagag caactggcaa acatcgcgcg
    20041 cggtggttat gtgctgaaag actgcgccgg tcagccggaa ctgattttca tcgctaccgg
    20101 ttcagaagtt gaactggctg ttgctgccta cgaaaaactg actgccgaag gcgtgaaagc
    20161 gcgcgtggtg tccatgccgt ctaccgacgc atttgacaag caggatgctg cttaccgtga
    20221 atccgtactg ccgaaagcgg ttactgcacg cgttgcggta gaagcgggta ttgctgacta
    20281 ctggtacaag tatgttggcc tgaatggcgc tatcatcggt atgaccacct tcggtgagtc
    20341 agctccggca gagttgctgt tcgaagagtt cggcttcacc gtcgacaacg tcgttgcgaa
    20401 agcaaaagaa ctgctgtaat tagcatttcg ggtaaaaagg tcgcttcggc gacctttttt
    20461 attaccttga tatgtccgtt tgcggacaag caatagataa agcgtgttgt agatcacaaa
    20521 tatttatatg caataaatat caattatgta atatgcatca cgatatgcgt attgacattt
    20581 gttgttatat ctataactca atgttatata agaaattaat aattcactgt tttcaaaaca
    20641 ccggtttccc tgctcaattg ctttcattaa accgcacgag tattttattt ttaccgccgg
    20701 acggtaccct aaaaataaaa taaggataat ttatgcggct tagtgactat tttccagaat
    20761 catcaatctc agttatacat tcagcaaaag attggcagga agctatcgat ttctcgatgg
    20821 tatcattgct ggataaaaac tatatcagcg agaattacat tcaggcaatt aaagattcca
    20881 ccattaacaa tggcccttat tatattctcg caccaggcgt ggcaatgcct catgcgcgac
    20941 cggaatgtgg ggcgcttaaa accgggatgt ctttgacatt acttgaacaa ggtgtttatt
    21001 ttccggggaa tgacgaacca attaaattac tcatcggact ctctgctgtc gatgccgatt
    21061 cgcacattgg cgctattcag gcgttaagtg aattactgtg cgaagaagaa atactcgaac
    21121 aactcttaac agcatcatca gaaaaacaat tagcggacat tatcagccgc ggataattac
    21181 tctctcctct acaggataat attatggaaa acaagtctgc tcgtgcaaag gtccaggctt
    21241 ttgggggctt tttgactgca atggtcatcc ccaatatagg tgcttttatt gcctggggct
    21301 tcataactgc gttatttatt cccaccggtt ggctgccgaa tgaacatttc gccaaaattg
    21361 tcggcccgat gattacctat ttattgcccg tgatgattgg ttctactggt ggtcatctgg
    21421 tcggcggtaa acgcggggcg gtcatgggcg gaataggtac tattggtgtg atcgttggcg
    21481 cagagatccc gatgttcctt ggctcaatga tcatggggcc gctcggtggg ttggtcataa
    21541 aatatgtcga taaggcactg gaaaaacgca tacctgccgg ttttgagatg gttatcaata
    21601 acttctcatt aggtatcgcg gggatgctcc tttgtctgct gggttttgaa gtgatcggcc
    21661 cggcggtgtt aattgccaat actttcgtca aagagtgtat cgaggcgctg gtacatgcgg
    21721 gttatctgcc tctgctgtca gtcatcaatg aaccggcgaa agtgcttttc cttaataatg
    21781 cgatcgatca gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat
    21841 ccatcttctt tatggtggcc tctaacctag gtccgggcct ggggctgctg ctggcgttta
    21901 ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc
    21961 tcggtgggat ccacgaactg tatttcccat atgtgctgat gaagccgctg actattattg
    22021 ccatgattgc gggcggtatg tctggtacct ggatgtttaa cttactggac ggtggtctgg
    22081 tggctggccc aagtccgggg tctatctttg cttacctggc actgacgccg aaaggctcgt
    22141 tcctggcgac aattgccggt gttacggtag gtaccctggt gtcctttgct attacttcgc
    22201 tgatactgaa gatggaaaaa acggtggaaa cggagagcga agatgagttt gctcagtcag
    22261 ccaatgcggt taaggcgatg aaacaagagg gtgcattctc gttaagcagg gttaagcgta
    22321 tcgcctttgt ttgcgatgcg gggatgggct ccagtgcgat gggcgcgacc accttccgta
    22381 aacgcctgga aaaagcgggg ctggcaatag aagtaaaaca ttacgcgata gaaaacgtgc
    22441 ctgcggatgc cgatatcgtc gtcactcacg ccagtctgga agggcgcgtg aaacgtgtga
    22501 cggataaacc gctgatattg attaataact atattggcga tccaaaactc gacactttat
    22561 ttaatcaatt aaccgccgaa cataaacact gattacagag gtaaaaatga aaaccaaagt
    22621 tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc cagaaattac
    22681 cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga cctggaaagc
    22741 ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc atccggtcat
    22801 taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca ctggcaaata
    22861 taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg gatattcagc
    22921 aggctacagc tacgaatatt ttggcggtaa cgccacctat atgattattc ccgaaatcgc
    22981 cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg cgtcgctggc
    23041 agagcctatg tgctgcatta ttggtgctta tcatgccaat tatcacacca cgcaatatgt
    23101 ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg cgtgtgcggg
    23161 tccgatgggc attggcgcta tcgattacgc cattaacggt ggcatacaac cgtcacgggt
    23221 ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg cagaagctgc tgccggtgga
    23281 tctggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga tgagcgatcc
    23341 ggtccagacg ctgcgggcgc tgacaggaga tgtcgggttc gatgacattt ttgtttatgc
    23401 ggcggtgcct gctgtcgttg agatggctga cgaattactg gcggaagatg gctgtctgaa
    23461 cttctttgcc gggccgacgg ataaaaactt caaagtgccg tttaatttct acaacgtcca
    23521 ttacaacagc acgcacgtag tcggtacatc cggcggttca acggacgaca tgaaagaggc
    23581 gattgcgtta agcgccacgg ggcagttgca gccgtccttt atggtcacgc atatcggtgg
    23641 gctggatgcg gtgccagata ccgtgctcaa tctgccggat atccctggcg gtaaaaaact
    23701 catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg aaaaaggcaa
    23761 aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg gcatctggaa
    23821 tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg aggccgcgca
    23881 atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt ggctgcctgg
    23941 ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac cgcgatgcgc
    24001 caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg agaaattgac
    24061 catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc agaagtcgat
    24121 atcgcggttg acccgattga aggcacgcgg atggtagcga tggggcagag caatgcgctg
    24181 gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatac gtatatgaaa
    24241 aagctggttg ttaatcgact ggcggcgggc gcgattgatc tgtctttgcc tctggcggac
    24301 aacttgcgca acgtggcgaa agcgttaggt aaaccgctgg acaagctgcg catggttacg
    24361 ctggataaac cgcgcctgag tgccgcgatt gaagaagcaa cacagctggg cgtgaaggtt
    24421 ttcgctctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca ggataatcct
    24481 tacgacgtga tgtacaccat cggcggtgcg ccagaaggcg tgatttctgc ctgtgccgtt
    24541 aaagcattag gtggggatat gcaggccgag ctgattgact tttgtcaggc taaaggggat
    24601 tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgtaaggc aatgggcgtt
    24661 gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg ggaacgatat cctctttagc
    24721 gctacgggcg tgacgggcgg cgagttggtg aacggtatcc aacagacggc gaatggggtg
    24781 cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat agactccctg
    24841 cattgatggt gatctatcgg aagaccaatg gcgacgctga cagaagatga tgtgcttgag
    24901 caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc tattttgctc
    24961 caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga gatcgtcgaa
    25021 tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga tgttgcgctg
    25081 cgtttgattt atgcgctggg gaaaatggat aaatggctgt atgccgatat cacgcacttt
    25141 tcccagtact ggcattacct gaacgaacag gatgaaacgc ccggatttgc cgatgacatg
    25201 acctgggatt ttatcagcaa cgtcaatagc ataacctgca atgcgacgct ctatgacgcg
    25261 ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt tagcggaatg
    25321 gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac gccgtgaaaa
    25381 ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa gagatagaac
    25441 gcgtgcataa accgttgctg cgtatgctgg cggccttaca gacggtaagt ccgcagcgac
    25501 gtacggtggt ttttctttgc gctccgccgg ggacgggtaa atctacctta actaccttct
    25561 gggaatatct cgcgcaacaa gaccctgaat tacccgctat ccagacgctc ccgatggatg
    25621 gttttcacca ttacaatagc tggctggatg cgcatcaatt gcgccccttc aaaggcgcac
    25681 cagagacatt tgacgttgcg aaactggcgg aaaatctgtg ccaggtagtg gaaggggatt
    25741 gtacgtggcc gcagtatgat cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta
    25801 ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct ggacgacgag aagtggtgcc
    25861 agcttgcgca attttgtgat ttctcgatct ttatcaacgc gccagctact gcgttgcggg
    25921 aacgactggt agggcgcaag ctggcgggag gggtatcgct ggcagacgca gaagcttttt
    25981 atgaccgtac cgacgggccg aatgttcgcc gggtgctgga agagagtctg cctgcaaatt
    26041 tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaacgt tcctgattag
    26101 accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact
    26161 gattgctacg acgctcgtgc ctcgctggcc gcacttctcc agccagcttt caaacacgct
    26221 caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg gcggagagag
    26281 tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat ccaacgggtg
    26341 cgagttggca gtgtctgcta actgacaaca ttgccatggg tacgttgagt gatttcatcc
    26401 gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt gctgtgaaaa
    26461 agttggtttt ccgcttcctg aaacagtacg ccaacctttg ccgcgcgctg gcggtttttc
    26521 aggtttttca tcgcctgttg ttgcagcata accgtaccgg ctgtgggcct aagaagacct
    26581 gccattactc gtaacagcgt cgatttccct gcgccattgt caccggtcag cgccaaccat
    26641 tcaccttgtt tgagttgcag agaaatgtcg caaagacaat ccgttgccgc gccaggccag
    26701 cgataagata tcttatttaa cgttaacata gtggctggag cgctccgttt tgcaattgcc
    26761 acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg ttcaaaaaga ataatcagcg
    26821 aaccacgttc aaacgcccag tgttgtaagc gctgcaacag catttcgctg gcctgcggag
    26881 tcaggcggct aaaggcttca tcaagaatta agagctttgg ctgcatcgcg atggcgcagg
    26941 cgatgaccac gcgctgtgtc tctccaccgg agagtgttgc cgggtgacga tggcgcaacg
    27001 gctggcactc ggtcagagtc agggcggcat caatccgcgc cataatctct gcttcagcaa
    27061 gacacagatt ttctggccca aaggcaactt cttcttccac gctaaaggta cagccggaga
    27121 gctgcaaata gggcgattgc tggacgagct ggatggtggc ggattgttca ttaagcggga
    27181 gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt tatctctcct ggcagaaagt
    27241 ccggatacca gcccgccatc agctgtgcca gcgtactttt gccactgccg ttgtcaccaa
    27301 agatggcgac catccccggt ttgacatagt gaaagtcata acagaacgga ggatgcgttg
    27361 agtgtgtcgg gcaataacgg aactgttcta acgtaaccat atccacgctc cgaattctgt
    27421 cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt agggtgctat cggctggtgc
    27481 ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc atatccagcg ccgcaccacg
    27541 gatagccaga tcgtttagcg cattgtgtgt tagcggaata attaacgcgg gcatcgcacg
    27601 taaacgctga taccagccct catccagcgg cacgccacgg gcacgctgcg cttcatgaat
    27661 aatcgctaat tgccgtttta actgctcaac gaccaataac ggcccggcaa acagataagc
    27721 gacgcctggc ggcaggcgag aagcaaacag agcgcgaata aaacattgca cggggacgta
    27781 ctgcatccac aactgtgagg ttgaaacaat cgccagaatc cgcaaccaga gcgtaatagc
    27841 atgcgaccac cgttctggcg agcgtggcgt gccgcttagc cattctggta gccagccacc
    27901 gtgtaccagc cacagcccgg ctcccagcga gaacatcagc cagacgacat attttgcccg
    27961 ccgtcgcgtg gcctttagcg caataaggca gaaaaaggtc gccgcgctat agatcggcaa
    28021 aatggtctgc gcgggcagga taagcgtggt gcaggccgcc aatgcccaca gtgttaatga
    28081 ggtaaatgga tgcatttagc gcacggcagc cattgccgga aaatggcgcg tggtgcgaat
    28141 cggcagctgg cgcagtaaaa gccagacaat caccgctgta agaattttat cgaccaggtt
    28201 cgcgcctatc accgtaatcg ccaccgactc caccaggttt tgccccatcg agtgcatcca
    28261 ggcaacgaac aaatcggctc cgctaccggt gacaccgccg aacagcgcag tacgcagcgg
    28321 tacagcgacg accgttaccg caagcgtaat aatcacgccg ctgacaacga cttttggcag
    28381 ggtgcgaaac caacccgctc gcgccagcca accggcgacc aggccgatga ccatcgcgac
    28441 gggtgcaaat gcggcggcga tcgggtcggt cagcaatccc cagagtaaat tggtcagtaa
    28501 gccggtcagc attccgatca ccggaccaag cagtaccgcg ctaataagtg tgccgatgga
    28561 gtcgagaaat ataggtaatt tgaccatgct ggccagttgc ccgccgatca tattgatggc
    28621 gatagatata acgatcaaca ccagtgcctg actggaaaag tggctacgcg ccataagaaa
    28681 acctctttga aagtagttat ttaacggggg aaatatgcaa ctcgtcgtaa ccagaaagcg
    28741 cgcaaggttg gcggctaaac gccagttcct caaggctacc gatcaccagt tcaagcgtgg
    28801 tccgcacggt acagccgggc atcatatgac cgccaagcat cgtgccgtgt ggatcggaaa
    28861 cgcagagatg aagatgctca ccactctgtt ccagcgtgcc gtttagtgag atcacttcga
    28921 atgtaccgtt caggagcgtg gtgccttctt gcccggcata gcgcaaggcg acgtcagtca
    28981 ggctaccggt acagcctgca atccaggcgg catgaagttg atgctgctgt gcaaaagcgt
    29041 gtagctggga aaaaacttcc tgcccgggga ggaggcgcag tgcgtaaaag cgtgcggagg
    29101 aagaatatgg gtagggcaat gtcatcattg gagtactccg aaactcggac gtattgcgga
    29161 taatatcgac gttgcatcag tcatattctg atataagtca gggtttgtga cggcacaacg
    29221 aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg aataaattcc acaggggcgg
    29281 ggctgaaaac gtgacgtaag tcagataatt gctttctttg actggacaat cattcctttt
    29341 attccacgtt tcgcttatcc tagctgaagc gtttcagtcg attaaatgtt cgacaattaa
    29401 ccaatcagtc gcagtttgcg acaggtaagg tttccccgga cgatttgctg gattactctg
    29461 tctggcaaat ttgctcaggg aaaaccttgc aggagatcta tgaccgtacg cgtagcgata
    29521 aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt tgtatgaatc cggacgccgg
    29581 gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg ctgcgggcat ggcgcatttg
    29641 ttgaaatatg acaccagcca tggccgtttt gcatgggaag tacgacagga acgcgatcaa
    29701 ctttttgttg gtgatgacgc catccgcgta ttgcatgaac gttcactgca atcgctcccc
    29761 tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg gcgtatatgg ctcccgcgag
    29821 catggcgaag cgcatattgc cgccggggcc aaaaaagtgc tcttttcaca tcctggcagt
    29881 aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg atcaacttcg tgcggaacac
    29941 cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa ttcccgtcat caaattgtta
    30001 gatgatgcgt acggtattga gtccggcact gtgaccacaa ttcactccgc catgcacgat
    30061 caacaggtta ttgatgcata ccatcctgac ctgcgtcgca cccgggcagc cagccagtcg
    30121 atcattccgg tcgatactaa actggccgcc ggtatcacac gattttttcc gcaatttaac
    30181 gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa atgtgacggc aatcgattta
    30241 agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca acctgttgct gcaaaaagca
    30301 gcacaaggtg catttcatgg tatagttgac tatacggaat tgccgttggt ctctgtagat
    30361 tttaaccacg atccgcacag tgccattgtc gatggcaccc aaacccgggt cagtggcgca
    30421 cacctgatca aaacgttggt ctggtgcgat aacgaatggg gctttgctaa ccgaatgctc
    30481 gacacgacgt tagctatggc tactgttgct ttcaggtaag acgcaagcag cgtctgcaaa
    30541 acttttagaa tcaacgagag gattcaccat gtctgtaatt aagatgaccg atctggatct
    30601 tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag acgggaaagt
    30661 aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc gaactggccc tgaaacaagg
    30721 cgcaaaagtg atggtaactt cccacctggg tcgtcctacc gaaggcgagt acaacgaaga
    30781 attctctctg ctgccggttg ttaactacct gaaagacaaa ctgtctaacc cggttcgtct
    30841 ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt gaactggttg ttctggaaaa
    30901 cgttcgcttc aacaaaggcg agaagaaaga cgacgaaacc ctgtccaaaa aatacgctgc
    30961 actgtgtgac gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc aggcttctac
    31021 tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg cagctgaact
    31081 ggacgcgctg ggtaaagcac tgaaagaacc tgctcgcccg atggtggcta tcgttggtgg
    31141 ttctaaagta tctaccaaac tgaccgttct ggactccctg tctaaaatcg ctgaccagct
    31201 gattgttggt ggtggtatcg ctaacacctt tatcgcggca caaggccacg atgtgggtaa
    31261 atccctgtac gaagctgacc tggttgacga agctaaacgt ctgctgacca cctgcaacat
    31321 cccggttccg tctgatgttc gcgtagcaac cgagttctct gaaactgcac cggctaccct
    31381 gaaatctgtt aacgatgtga aagctgacga gcagatcctg gatatcggtg atgcttccgc
    31441 tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg gtccggttgg
    31501 cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc gtggctaacg ctatcgcaga
    31561 cagcgaagcg ttctccatcg ctggcggcgg cgacactctg gcagcaatcg acctgttcgg
    31621 cattgctgac aaaatctcct acatctccac tggcggcggc gcattcctcg aattcgtgga
    31681 aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt aaaaaatcac
    31741 agggcaggga aacctgccct tgtttcagcg cgcttttaga gcacgcattt tttcatactc
    31801 taaataattc gagttgcagg aaggcgacaa gagagtgaat ccccaggagc ttacataagt
    31861 aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gatgagtata
    31921 aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta aaacctggcg
    31981 taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac aacttcgcac
    32041 tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtactggaa accgctgcta
    32101 aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt atcgctggta
    32161 aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc tctggtgcgc
    32221 atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac actgaccact
    32281 gcgcgaagaa actgctgccg tggatcgacg gtctgttgga cgcgggtgaa aaacacttcg
    32341 cagctaccgg taagccgctg ttctcttctc acatgatcga cctgtctgaa gaatctctgc
    32401 aagagaacat cgaaatctgc tctaaatacc tggagcgtat gtccaaaatc ggcatgactc
    32461 tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac agccacatgg
    32521 acgcttctgc actgtacacc cagccggaag acgttgatta cgcatacacc gaactgagca
    32581 aaatcagccc gcgtttcacc atcgctgcgt ccttcggtaa cgtacacggt gtttacaagc
    32641 cgggtaacgt ggttctaact ccgaccatcc tgcgtgattc tcaggaatat gtttctaaga
    32701 aacacaacct gccgcacaac agcctgaact tcgtattcca cggtggttcc ggttctactg
    32761 ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc gataccgata
    32821 cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa gcttatctgc
    32881 agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac tacgatccgc
    32941 gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa gcattccagg
    33001 aactgaacgc gatcgacgtt ctgtaagata ttcctttctg cttatctcaa ggcccgctct
    33061 gcgggtcttt ttttcgccaa aagcaaaatt gcctgatgcg ctacgcttat caggcctaca
    33121 cgtatcctgc aatatattga atttgcaggt ttttgtaggc cggataaggc gttcacgccg
    33181 catccggcat gacaaagcgc acattgatat ctgtctgagc cccgctctgc ggggattttt
    33241 tcgccaaaag caaggtgatt cagatgagaa atctgtgatc tatttggcaa aattatgctt
    33301 tattgtttac ccttgtcaga ctgcccgtca taaggcggcg gagtgtattt ctccattttg
    33361 agtcagttga aaaggaatat tgaatggaag atttgaatgt tgtcgatagc ataaacggcg
    33421 cgggaagctg gctggtagct aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg
    33481 cggcactcgc gatcatcatc gttggtttga ttatcgcgcg gatgatttcc aacgcggtga
    33541 atcgcctgat gatctcccgt aaaatcgatg ccactgttgc tgattttctt tctgcattag
    33601 tccgttacgg tattatcgcc tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa
    33661 cagcgtcagt cattgctgta ctcggtgccg caggcttagc tgttggtctg gctttgcagg
    33721 ggtcactttc taacctggcc gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg
    33781 gagaatatgt tgacctcggc ggcgtagccg gtactgtgct gagtgtgcag attttctcca
    33841 ccaccatgcg tactgcagac ggtaaaatta tcgttattcc gaacggtaaa attattgccg
    33901 gaaatattat taacttctcc cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg
    33961 cgtatgattc cgatatcgat caggttaagc agatcctgac caatattatc cagtctgaag
    34021 atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga
    34081 ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct gcaaaacgtg tactgggatg
    34141 tgctggagcg tattaaacgt gaatttgatg ccgccggtat cagcttcccg tacccgcaaa
    34201 tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag
    34261 ataatactct ctggcctctc tcttattagt tattctgatt gccaattaat attatcaatt
    34321 tccgctaata acaatcccgc gatatagtct ctgcatcaga tacttaattc ggaatatcca
    34381 acgtgttttc ttattacttt caaggtcttg ctcttggggc agctatgatt ctgccgctcg
    34441 gcccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac attatgattg
    34501 ccttactttg cgctatcagc gatttggtcc tgatttgcgc ggggattttt ggtggtagcg
    34561 cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc gtagtcttct
    34621 tgctctggta tggttttggc gcttttaaaa cagcgatgag tagtaatatt gagttagcta
    34681 gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc caccatgttg gcagtgacct
    34741 ggctgaatcc gcatgtttac ctggatactt ttgttgtact gggcagcctt ggcgggcagc
    34801 ttgacgtcga accaaaacgt tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt
    34861 tctttggtct ggctattctc gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac
    34921 agcgcattat caatctggta gtgggatgtg tcatgtggtt tatagccttg cagctggcga
    34981 gagacggtat tgctcatgca caagccttgt tcagttaggc gctatctgat ggaaaaataa
    35041 aacagaggcg ctaagcttgc ctccagaggt cctgagtttt gggcaagtca attaacacgg
    35101 agagactaac gtgaagttca aagttatcgc cctggcggca ttaatgggta ttagcgggat
    35161 ggcagcgcag gctaacgaat tgccggatgg accgcatatt gtcacctccg gtacggcaag
    35221 tgtggatgcg gtgtcagaca ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga
    35281 tgccgctaca gccaagaaac aggcagatga gcgcgtcgca caatacattt ccttccttga
    35341 actcaatcag atcgcgaaaa aagatatcag ctcagcgaat ttacgcaccc agccagatta
    35401 tgattatcag gatggtaaaa gtatcctgaa aggctaccgc gctgtgagaa cggtggaagt
    35461 cacgctccgt cagttagaca aactgaattc cttgctggat ggcgcgctga aggcgggtct
    35521 taacgaaatt cgttctgtgt cgctgggcgt ggcgcagcct gatgcctata aagacaaagc
    35581 gcgtaaggca gcgattgata acgcgattca tcaggcgcag gaactggcga acggctttca
    35641 tcgtaaactg gggccggtat atagcgtgcg ctaccatgtt tccaactatc agcccagccc
    35701 aatggtgcgg atgatgaaag ccgatgccgc gccggtgtct gcccaggaaa cttacgagca
    35761 ggccgctatt cagtttgatg atcaggtcga tgtggtcttc cagttagaac ctgtggatca
    35821 acaacccgct aaaacacctg cagcacaata atacttgtag cccgacgtat tcgtcgggct
    35881 aatgccttta cccgatattg cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat
    35941 cacctatctt tgctcgcggt ttcatcaagg ataggctgac tgaaacggtt aagcggacaa
    36001 ggatgcgggg aggggggatg gacattttta tttcgaaaaa gatgcgcaat ttcattttat
    36061 tagcgcaaac caataatatt gcccgggcgg cagaaaaaat ccatatgtcg gcttcgccat
    36121 ttggtaaaag cattgccgcg ctggaagaac aaattggcta tacgctattt acccgcaaag
    36181 ataataatat cagcctcaat aaagccggtc aggaactgta tcaaaaactg ttcccggttt
    36241 atcaacgact ttctgcgatt gataatgaaa tccataactc agggcgtcgt tcacgagaaa
    36301 ttgtgattgg catagataat acttatccga caattatttt cgatcagttg attagtctcg
    36361 gagataagta tgaaggcgtg accgcccagc cagttgagtt cagcgaaaac ggcgttatcg
    36421 ataatctttt tgaccgtcag ttagatttta ttatctcccc acagcatgta tctgcacgag
    36481 ttcaggaact ggaaaacctg actatcagtg aattaccacc gctgcgattg ggctttctcg
    36541 tttctcgccg ttatgaagag cgtcaagagc aagagctgct tcaggagctg ccctggctac
    36601 aaatgcgctt tcaaaaccgg gctaacttcg aagcaatgat tgatgcgaat atgcgacctt
    36661 gtgggatcaa tcccaccatt atttatcgtc cctacagttt tatggccaaa atcagcgccg
    36721 tggagcgcgg acattttctg acggtgatcc cgcattttgc ctggcgactg gtgaatccgg
    36781 caacgctgaa atattttgat gcgccgcaca ggccgatgta tatgcaggaa tatctttatt
    36841 caatcagaaa tcatcgttat accgccacga tgcttcagca tattgctgaa gatcgtgacg
    36901 ggacaaacca ttaacccagc atcgagccgg ttgcaattaa attacggtgt aagtcgaaga
    36961 cgtggctaag atcgtggtga atatgtccgc cgggcgcatt ttccagatag cgatgcagat
    37021 aatcccgata cataggatgt gcacaattat caatgatagt gcgggcgcgt tgaagcgggg
    37081 aaagaccgcg cagatcggcg atcccttgtt cggtgatgat cactttgacg ctgtgttcgc
    37141 tgtgatcaac atggctgcac attggcacga cggttgagat cttgccttct ttagcaatcg
    37201 acggagccat aaagatcgac agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc
    37261 cgttcatcag atcgaccccg gctacgtgcg ttgagttggc atgcccgtaa atatcaaact
    37321 ccaggccgac gttcagagcg atgacgccca gacgacggat gatttccggg ttattggaaa
    37381 tctcctgcgg acgcaacaca atgcggctgg caaagtaatc catattgtcg taaatcttgc
    37441 gcagggaatc ggccgagatt gtcaggctgg aggcgctggc cccgctgatt ttgccggttt
    37501 ccagtaaatg caccaccgat tcctgtagca cttccgaata catcataaac ggaggaattt
    37561 ccgggttttc ccccagacgc gccattaccg cattattgat attgcccacg ccactttgca
    37621 gcggcagaaa ttccggcgga atacgcccat gcgccatttc ctgcaataag aacgtgacca
    37681 cgttatcggc aatctgctgg cacatgggat tttgcttatc cagcatatta ccggcgtcgg
    37741 gcaagttggt ttccacgacg gcgacaatct ttttcggatc gatttgcaca tagcgggtac
    37801 cgacgcgatc cattgcatgg aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa
    37861 tcacaatatc cgccagttct gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca
    37921 ctttcttcgc ccgcagcagc catgtcggcg cattaccgat cccgctggtt aaccagactc
    37981 gaccatccgg tgccagtgcc gatgcttcga tgacggcaac atcaatgtcg ccgaagaaac
    38041 cgtaattgac catttgcgcc acttcgctca aatgcaggtc aacgaaactc accgcgccct
    38101 gattgatctt tttacgtaaa ccggacgatg tttgatatgg cgcacgccag gaaacagcat
    38161 cggcgtcaga aagtacatcg tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa
    38221 tttgatacgg ctttttggcc tcatgctgtt cgttagctct gcgggcaatc gcggtcggta
    38281 aggctttcgg cgaacccgct ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga
    38341 taatttctgc cgcttcatcg gcggtcatcc ttgtccactg agtttccatt tttactcctt
    38401 cgttcgcagg gattaatgac caacgaaatt aggtttacgt ttttcgagga aagcgttcat
    38461 cccttcctgg taatcttcgc tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa
    38521 ttcatcggag ttcatggtgt gcgcttcgcc cagtacacgc agctcttctt tgataacagc
    38581 aatggctaac ggcgctttct cagagatgtg gtgcgccatt tgtaaggtga aatcttccag
    38641 ttcttccact tccacaacat ggttgaggat gccgacagcc aacgcgcgct gggcggtgat
    38701 tggcgaagcg gtaaaaatca gttctttgac aatgtggaag cccgcgtcgc gggtcaggtt
    38761 gtgaatgccg accaggttat acgggacgcc gaggtttaca ggcgtcattg agaaggttga
    38821 ggtactggcg gcgataatca gatcggaact catgatcatt tcaaatgcgc caccccaaac
    38881 actaccttcc accatcgaaa tgatcggttt cgggaatttt tggatcatgc gggtgatttg
    38941 acgcaatgga tcatcatagg agagcggatc gcgaccgcca gacggcagtt cgtgaatagc
    39001 gtggcctgcg gagaagactt tggatccact cggcgcgcgc aaaatgatac agcgaatttc
    39061 cggccggttg agatcgctta atgcctgcat aagatcatca ataaagactt tacttaaggc
    39121 attaagtttg cggccatagt taaactcaat gaccgccact ttgttgatag tgacaacgtt
    39181 aacatactga taagacataa aaattccttt aatcaaaata ttgcgtctgg ataaattcac
    39241 tgagctgccg caggccggtg cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct
    39301 ggcggtaata gcgatcgaaa tcttcattcg cgaacaggtg attcagtact tcttcttcgg
    39361 tctgcttacg cagccattcc accgattgtt gttgccgcac ttgttgtaaa cgaccactgg
    39421 cagttagcgc ggttttgaag tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt
    39481 ccagtgcgct acaagtcaga acccgtggct gccattcgtc gtatttacgt cgcagaatat
    39541 gcagggcact ctcgtacata tgccgggcaa tggcgacatt ggtatggtta tcgccatcgt
    39601 ctttgttgat aacgatcaga tcagccactt ccatcagccc ttttttaatg ccctgcagat
    39661 catcgccgcc accggcaatt tgcaacgaga taaaacagtc caccatgcgg gcgacttctg
    39721 tttccgactg cccgacgcca accgtttcga caatcactac gtcataaccc gctgcttcgc
    39781 ataacagcat taattcccgc gctcgctgac tggcaccgcc cagatgaccg gaggatggta
    39841 ccgggcgaat aaacgccgct tcggcacgcg ccaggtcatt catgcgggtt ttatccccga
    39901 gaatgctacc gccagtgacc gggctgctgg gatcgaccgc aataaccgcg acctttaatc
    39961 cctctcgaat caacaacatg ccaaaggcct caagaaaggt acttttcccc gcgccggggg
    40021 tgccggtaac gcccagtcgc agggtgttac cgcagtacgg cataatggca tcaagcagct
    40081 gcgtacttag tgcctgatga cgcgggtgac ggctttccac cagcgtcatg gcctgggcga
    40141 gtgtggcacg ctcaccctga cgtaagcggc gaatactttc tgccagcgtg gcttcattaa
    40201 tcatgatgct ggcttatcag attcagtacg tcgcgcacac tgtcgagcat aggtgtacct
    40261 ggaccataaa tcgccgccac gccgcgctct tgcaggaagg cgtaatcctg cggcggaatg
    40321 acgccacccg cgaccacgca gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt
    40381 tccgggatca gcgttttatg accggcagcc agtgaggatg cgcccactac gtgaacgtcg
    40441 ttttctacgg ccaggcgggc gatctcttca ggtgtagaga acatcgggct taaatctacg
    40501 tcgaaaccga gatcggaata ggcgctggcg atcactttcg cgccgcgatc gtgtccatcc
    40561 tggcccatct tagcgatcag aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc
    40621 gtttgcgcaa caatggcatc gaactcggag gccgatttct cagactgatg atagctttgc
    40681 gcaatcacgc cggtaacaca ctggcttggc accagataac ggtcgaaagc gacttccagc
    40741 gcatcggaaa tttcacccag ggtggcgcga acgcgagcgg cattaacagc ggcagccagc
    40801 aggttttcgt tatgctgtgc ggcgtgagtc agggcgttca acgcggcggt tacggcggca
    40861 tcatcacggg tggcgcgaat gcgttccagc gaagcaattt gctcgttacg caccatcacg
    40921 ttgtcgatct caagtacatc ggtttcgtct tcgtgatcca gtttgtactt gttgacacca
    40981 acgatgacac gcttgccctg gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc
    41041 attcgttttg gcagacctgc ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt
    41101 tggataatag ctctggcttg tttgacgatt tgatcggtca gcgactcaat gtaataggat
    41161 ccggccagtg gatcgacgat gcggcagagt tctgattctt cctggatgat gatctgggtg
    41221 ttgcgggcaa tgcgtgctga gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg
    41281 gtatgcagtg actgagtacc gcccagcgtc gcagccagcg cttcaatggt ggtgcggata
    41341 acgttgttat acggatcctg ttcagtcagg ctccagcctg aggtctggca gtgggtacgc
    41401 agcgccagtg atttcgggtc ctgtgcgcca aatccactga ccgcttcgct ccataaataa
    41461 cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccgatgcc gaagaagaac
    41521 gacaggcgag gagcgaagtc atcaattttc agtccggcag agattgctgc tttgatgtac
    41581 tcaatcccat cagcgagcgt aaatgctacc tgctgcacgc agttggcacc cgcttcaccc
    41641 atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga acaccaggcg
    41701 atgatgtcgg cgataatgcg cattgacggt tttggcgggt aaatataggt gttgcggcag
    41761 aggtactctt tgaggatatc gttctgaatg gtgccggtca gtttatcagg tgtaacacct
    41821 tgctcttccg cggcgatgat ataaaacgcc agtactggta gcactgcgcc attcatggtc
    41881 atcgaaaccg acattttatc cagcgggatc tggtcgaaca ggactttcat atcttccacg
    41941 gtgtcgatag cgacgcccgc tttgccgacg tcacccgcca cgcgtgggtt atcggagtcg
    42001 tagccacggt gggtggcaag gtcaaacgca acggaaagac ctttttgccc ggcggccagg
    42061 ttacggcgat aaaaagcgtt ggactctttt gctgttgaaa aaccagcata ctgacggatg
    42121 gtccacggtt gggcggtata catagtggca cgcgggccac gaacgtaggg cggcaaacca
    42181 ggaagggtac ctgtcacctc cagattatcg agatcggctt cggtatacag cggcttgatg
    42241 gcgatccctt ccgcggtttg ctgaaccagc gagtcgacag ttttctcccg acggctcaat
    42301 tccttgttgg caagctgttg ccactcctgc tcgttagaca ttatttgctc cgtaaaacgc
    42361 aatcaaaatg agggtatttg cgcttacggt gagcttttta cgtgggtttg tcgccagcaa
    42421 aaaaaatgca ttcagacgtc gttttttgcc gcctgagatt tgtctatttt gtgatgtgat
    42481 tcaaggaata caaaaagaag tgaggataac gcctgatatg tgctatcagg cattatttga
    42541 tggattaatc ctgacgaagg actttgtgac cataatcgag caacgcatca gtgactttac
    42601 gcatcatgcg gctttcagga gcaaagcggt gccagtagag catccggcgt tgaaatagcc
    42661 ctggcgttaa gtcaatcagt tcaccgctgg ctagttcttt ctcgatttgc aggtgcggga
    42721 tcatacagca ggtggtgccc tggcgagcaa gttgcacgaa cgcttctgaa gaattaacga
    42781 tatggcaggg cacgctgcct ggaggcagat cgaagttttg ctgcaaaaag gcctggtgca
    42841 tatcgtcaag atggtcaaag gcgaccacag gtgctttcaa taatgccgaa cgcgttacgc
    42901 cgttagggaa atatttttcg gcaaagggtt ttgagctgac gaacagatag tcgagcgcac
    42961 caagtttatc gacaagacaa ctcggcagcg cctgatgttg aatactcacc gcgccgacca
    43021 cttcgccgcg gcgcagacgt tcctgagtgc gggtttcatc ttctacctgc aagttgaggc
    43081 ggataggcga atcagccaac acaggagcca gtgcaggaag caaccacgtc gccagactgt
    43141 cggcgttgac cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc
    43201 actcttcttc cagcaactcc acctggcgca gcagtgccag cagtttttgc ccttgttccg
    43261 tcgggcgcgg cggtacggta cgcaccaaca gcggctgccc gaacatattt tccagttgct
    43321 taatgcgctg tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc
    43381 ctcgttcacg tatcaccgca tccagtgcct gtaatgttct gtagtccggg cgtttcattg
    43441 ctgttgtctg ctcctggtaa atgctttttc ctgcactatg acacaatttt gtgtcaggtt
    43501 gcatataccg aagccgtcgg cgggtaacat ttgtattcgt ggttaaacgc gcagcttaaa
    43561 agatcaggga tttgcgctaa tgcatttcgc tcagcgagtt cgcgccctgg ttgtgttgaa
    43621 tggcgtggcg ttattgcctc tgttcgcacc gtggagcggc atacccagac cgttgtatgc
    43681 tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga tattttatgg atgagttaac
    43741 cacgcggctt gccaacgggg tctgaatcgc tttttttgta tataatgcgt gtgaaatttc
    43801 ataccacagg cgaaacgatc atgacgcagg atgaattgaa aaaagcagta ggatgggcgg
    43861 cacttcagta tgttcagccc ggcaccattg ttggtgtagg tacaggttcc accgccgcat
    43921 actttattga cgcgctcggt acaatgaaag gccagattga aggggccgtt tccagttcag
    43981 atgcttccac tgaaaaactg aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg
    44041 acagccttgg catctacgtt gatggcgcag atgaaatcaa cggccacatg caaatgatca
    44101 aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc ttcggttgca gaaaaattta
    44161 tctgtattgc agacgcttcc aagcaggttg atattctggg taaattcccg ctgccagtag
    44221 aagttatccc gatggcacgt agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc
    44281 cggaataccg tcagggcgtg gtgaccgata atggcaacgt gatcctcgac gtccacggca
    44341 tggaaatcct tgacccgata gcgatggaaa acgccataaa tgcgattcct ggtgtggtga
    44401 ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct gattggcaca cctgacggtg
    44461 tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc cgttaaaaaa attctcttca
    44521 ttaaatttgg tgacatgtgt cacgttttta cctggctatt gtcgattgct ctaaataaat
    44581 cttctaaacc agcatattca tccaagaatt acctttgcgt gatatttcct caacatcgcg
    44641 acgcaaacgt tcatattgcc gcaatattat tttttgatat gttgaaaggc ggacgcaaat
    44701 ccgcacacaa catttcaaaa gacaggattg ggtaaatggc aaaggtatcg ctggagaaag
    44761 acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg
    44821 cagctggtta caccaacatc gaatttcaca aaggcgcgct ggatgatgaa caattaaaag
    44881 aatccatccg cgatgcccac ttcatcggcc tgcgatcccg tacccatctg actgaagacg
    44941 tgatcaacgc cgcagaaaaa ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg
    45001 ttgatctgga tgcggcggca aagcgcggga tcccggtatt taacgcaccg ttctcaaata
    45061 cgcgctctgt tgcggagctg gtgattggcg aactgctgct gctattgcgc ggcgtgccgg
    45121 aagccaatgc taaagcgcac cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag
    45181 cgcgcggcaa aaagctgggt atcatcggct acggtcatat tggtacgcaa ttgggcattc
    45241 tggctgaatc gctgggaatg tatgtttact tttatgatat tgaaaataaa ctgccgctgg
    45301 gcaacgccac tcaggtacag catctttctg acctgctgaa tatgagcgat gtggtgagtc
    45361 tgcatgtacc agagaatccg tccaccaaaa atatgatggg cgcgaaagaa atttcactaa
    45421 tgaagcccgg ctcgctgctg attaatgctt cgcgcggtac tgtggtggat attccggcgc
    45481 tgtgtgatgc gctggcgagc aaacatctgg cgggggcggc aatcgacgta ttcccgacgg
    45541 aacctgcgac caatagcgat ccatttacct ctccgctgtg tgaattcgac aacgtccttc
    45601 tgacgccaca cattggcggt tcgactcagg aagcgcagga gaatatcggc ctggaagttg
    45661 cgggtaaatt gatcaagtat tctgacaatg gctcaacgct ctctgcggtg aacttcccgg
    45721 aagtctcgct gccactgcat ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg
    45781 gcgtgctaac tgcgctgaac aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat
    45841 atttacaaac ttccgcccag atgggttatg tagttattga tattgaagct gacgaagacg
    45901 ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg taccattcgc gcccgtctgc
    45961 tgtactaatt ccccttctct gaaaattaac gggcaggtca ctgacttgcc cgttttttta
    46021 tcccttctcc acaccgttgc cgcgttgcgc attctacttt gcgagtctgc tccagaaaat
    46081 aaatcaaagt cagatcaatg cgttgtgttt atttaatggg gatttacgat ggcgggacag
    46141 catggggctg taacggtaaa gccctcaccg aagcgagggc ttaaaggaga agggttatga
    46201 tgcgacttgt catcatactg attgtactgt tactgataag tttcggagct tattaacagt
    46261 cagtctcagg ggaggagtca tcctccctta cccttactca ctaaattagg tcaaagaatc
    46321 aacgatgtca atcagggcga tgcggggtgc atcgccctga accgaacttt ttgatagcca
    46381 cttcatcgcc cacgtttact gccgtaatag atcctttttc ccaaaaatta tctctgtttt
    46441 gcatggggtt tttcgagcac gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg
    46501 ttatttaaag agagcttacg atagctgggc agcatggggc tgtaacggta aagccctcac
    46561 cgaagcgagg gcttgaagga gaagggttat gatgcgactt gccatcatac tgattgtact
    46621 gttactcata agtttcccag cttattaaca gtcagtcaca ggggaggagc aatcctccct
    46681 tacccttact cactaaatta ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg
    46741 tatcgccctt accactccca gactttcgac ggtgtaacca ccgcaggaag agggatatcc
    46801 cactcttcaa cggggagttt ttccaccaac tgacaatcat gcgcataacc caccggttgc
    46861 gttttatagt gctgccagtt ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg
    46921 cgctgaccgt actcatcaaa ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg
    46981 ggtagcacgt cacgcacatc cagttttggc tcatggatct tcaacctgtt catcaccagt
    47041 tcgctttgcg gatggtaatt caggaacagc aaattaccgg cactaaaggg atgcaaaact
    47101 ggaagatata cgcgcttacc ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt
    47161 tcaccatcaa aagagaggaa tacagcgacc gtatgcgcca tcaccaccgg gggataagtc
    47221 atcatccggg tagcggcttg ttgacccatt tcctgctgtt gttccggcgt taacgcacga
    47281 cgacgttgcc gaatcatttt gcggatttct tgtcgggata atgtcagtgg gagttctggt
    47341 agttgcgtca tggctggtgc cagataagaa gggaatctcc gagatgccgc cgcaggctgt
    47401 aacccttgaa cccttggttc aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg
    47461 gaccgagcat gctcaccaac cgcggagcgc cacattcttg tggtatgaaa tatcggctca
    47521 ggggactggc ccgcttgcga acatctcaga gaaattttgt cttcacggtt actctaccac
    47581 agtaaaccga aaagtgtcat tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt
    47641 caagtaacgc ttgttctatg gtctgctgca gcatccgaat acgctgttcc atacttgccg
    47701 cgtagtcacg agtcttcgct ttttcttgcg ctaactcata gctgatattc aatgcggcaa
    47761 tgaagaccaa ctgttcagta tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt
    47821 ggttcagatc gtccgctgcc tgattcaacg catccctttg gtcaggcggg cagttcacac
    47881 gcagtgaacg gccaaaaatt tggatatcga cgggttgtgc agacatgcca ccttcctgct
    47941 gattgactgc gctgccttcg tctttagacc catggtctgc gaaggggcga cactatagct
    48001 accctgatga gaagagacaa gcccttttct ggtccaccag ggcccaaagt ggtagcatat
    48061 catgaatatt cctccctttg acgacgaatg cttatgtcta tacagaacga aatgcctggt
    48121 tacaacgaaa tgaaccagta tctgaaccaa caagggacgg gtctgacccc tgctgagatg
    48181 catggtttaa tcagtgggat gatatgtggc ggtaacgatg acagctcatg gctaccgcta
    48241 cttcacgacc tgacgaacga aggcatggct ttcggtcatg agctggcaca ggcactgcgt
    48301 aaaatgcact ctgccaccag cgatgccctg caggatgacg gcttcctttt tcagctttat
    48361 ctgcctgatg gcgatgatgt cagcgttttc gatcgggctg atgcattggc aggttgggtc
    48421 aatcacttcc tgcttggtct tggcgttacg caaccgaagc tggataaagt gaccggcgaa
    48481 accggtgaag ctatcgacga tctgcgtaac attgcgcaac tgggttacga cgaagacgaa
    48541 gatcaggaag agcttgaaat gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg
    48601 ctgttatgcc acgacacctt tactcatccg caaccgaccg cgccagaagt acaaaaaccg
    48661 actctacact aaaaacaaaa aacgtaagga gagtgttatg agtgagatat cccggcaaga
    48721 gtttcagcgt cgccgtcagg ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat
    48781 ttttgctgca ccagaagtaa cacgtagcgc cgacagcgaa tacccctatc gtcagaacag
    48841 tgacttctgg tacttcaccg gctttaacga accggaagcg gtgctggtgc tgattaaaag
    48901 cgatgacact cataaccaca gcgttctgtt taaccgcgtt cgcgacctga cggcggagat
    48961 ctggtttggc cgtcgcttag gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc
    49021 actggcattc agcgaaatca atcagcaact ttatcaacta cttaacggcc tggatgtggt
    49081 ttaccatgcc cagggcgaat atacctatgc tgatgaaatc gtgaacagtg cgctggaaaa
    49141 actgcgtaaa ggttcgcggc aaaatctcac cgcaccggca acgatgatcg actggcgtcc
    49201 tgttgttcat gaaatgcgcc tgttcaaatc gccagaagag attgccgtac tccgccgcgc
    49261 gggagaaatc accgccatgg cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt
    49321 cgagtaccat ctggaaggcg aaattcacca cgaatttaac cgccacggtg cgcgctatcc
    49381 gtcctataac accattgtcg gcagcggtga aaacggctgc attctgcact acaccgaaaa
    49441 cgagagtgaa ctgcgcgacg gtgacctggt gttgattgac gcgggttgtg aatacaaagg
    49501 ttacgcgggc gatattaccc gcaccttccc ggtcaacggc aaattcaccc aggcccagcg
    49561 tgaaatctac gacattgtgc tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg
    49621 aacttccatt ctggaagtca ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa
    49681 actcggcatc ctgaaaggtg atgttgatga actgatcgct cagaacgccc atcgtccttt
    49741 ctttatgcat ggccttagcc actggttagg actggatgtc catgacgttg gcgtttatgg
    49801 tcaggatcgc tcgcgcattc tggaaccggg gatggtactg accgtagagc ctggtctgta
    49861 tatcgcgccg gatgcggatg tgccagaaca atatcgcggt atcggcattc gaattgaaga
    49921 cgacattgtg attaccgaaa ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc
    49981 ggaagaaatc gaagcgttga tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt
    50041 ggcggcatgg cgggcgcgac gctggcgctg gctatttccc ggttaagtca cggggcgctg
    50101 ccggtacatt tgattgaagc gactgcgcca gagtcacatg ctcatccggg ctttgatgga
    50161 cgagcgatag cgctggcggc gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa
    50221 tctctggcgg attgcgcaac tgccatcacc accgtgcatg tcagcgatcg tggtcacgct
    50281 ggatttgtca ccctcgccgc agaagattac caactggcgg cgctgggaca ggttgtcgaa
    50341 ttgcacaatg tcgggcaacg gctgtttgca ttgctgcgta aagcacctgg cgtaatgctg
    50401 cattgccccg atcgcgtagc taacgttgcc cgtactcaga gtcacgttga agtgacgctg
    50461 gagagtggcg agacgctgac gggccgcgtg ctgatagccg ctgatggcac ccattcagcg
    50521 ttagccaccg cgtgcggcgt tgactggcag caggagcctt acgaacaact ggccgtgatt
    50581 gccaacgttg cgacttccgt tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat
    50641 ggcccgctgg cgatgttgcc gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca
    50701 ctggaacggc gcgaagaggt gctgtcgtgg agcgacgaga agttttgccg tgaactccag
    50761 tcggcctttg gctggcgact tggaaaaatt acccacgccg gtaaacgcag tgcttatccg
    50821 ctggcgttaa cccgcgccgc cagagctatt acccatcgta ccgtgctggt gggcaatgcg
    50881 gcgcaaactc tgcacccgat tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg
    50941 agccttgcgg aaaccctgac tcaggcgcat gagcgcggag aagacatcgg tgattacggc
    51001 atattgtgcc gctatcagca gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg
    51061 gacagcctgg tacatctttt tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc
    51121 ggactgatga cgatggaatt attcaccccg gcacgcgatg tgctggcgca gcgcaccctc
    51181 ggttgggtgg cgcgttgaag gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc
    51241 cattgttggt ggcggcatgg tggggctggc ggttgcctgc ggcttacagg ggagcggctt
    51301 acgcgttgcc gtactggagc agcgcgtacc ggaacctctg gtggcggatg caccaccaca
    51361 actgcgcgtt tcggctatca atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg
    51421 gcaggacatt ctctctcgta gggccagttg ttatcacggt atggaagtgt gggacaaaga
    51481 cagttttggt cacatttcgt ttgacgatca aagcatgggc tatagccatc ttgggcatat
    51541 cgttgaaaac tcagtgattc actacgcgct gtggaacaaa gcgcagcagt cgtcagatat
    51601 cactctgttg gcccccgcag aattacagca ggtcgcctgg ggagaaaatg aaactttcct
    51661 gacgctgaaa gatggcagca tgttaacggc gcgtctggtg attggcgcgg acggtgctaa
    51721 ttcctggttg cgcaacaaag cagatattcc gctgactttc tgggattatc agcatcacgc
    51781 gctggtagcg accattcgca cggaagaacc gcatgatgcg gtggcgcggc aggttttcca
    51841 tggcgaaggc attctggcct ttttaccgct tagcgatccg catctttgct caattgtctg
    51901 gtcactgtcg ccagaggaag cgcagcggat gcagcaggca agtgaagatg aatttaatcg
    51961 cgcgttaaat atcgcttttg ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca
    52021 ggtgttccca ctgacggggc gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctggt
    52081 gggcgacgcc gcgcatacca ttcacccgct ggcggggcag ggagtaaatc tcggctttat
    52141 ggatgctgca gagctgattg ctgaactgaa acggttgcat cgtcagggga aagacatcgg
    52201 gcagtacatt tacctgcgcc ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct
    52261 ggctggtatg cagggattcc gcgatctgtt ttccggtacc aatccggcga aaaaactgct
    52321 gcgtgatatt ggtttgaaac tggccgacac gcttcctggc gttaaaccac aacttatccg
    52381 ccaggcaatg ggattaaacg atttgcctga atggctgcgt taaaaatttc tcttctgctg
    52441 tttatttgat acccatcaca ctttcatctc ccggtttttt cgccgggaga ttttcctcat
    52501 ttgaaataaa ctaatttcac ctccgttttc gcattatatt ttctaatgtc attatttttt
    52561 gatttagtgt tttttgacat ttttttagct cttaatattg ccttattcaa attagctttc
    52621 taatggcatc atctttatat agaaactggt gtgttttttg gttttttatt ctgtcgcgat
    52681 tttggcgttt tttaaccata agctaatgtg atgatcaatt ttaccttatg gttaacagtc
    52741 tgtttcggtg gtaagttcag gcaaaagaga acgattgcgt tggggaccgg gagtggctcc
    52801 gatgctgggt ttcgtggtga taatttcacc atgaaaagtt gtcagccccg cttattcaat
    52861 gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg cggcgctcgc
    52921 atggtggatt tccacggctg gatgatgccg ctacattacg gttcgcaaat cgacgaacat
    52981 catgcggtac gtaccgatgc cggaatgttt gatgtgtcac atatgaccat cgtcgatctc
    53041 cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc
    53101 accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg tgtgatagat
    53161 gacctcatcg tctactactt tactgaagat ttcttccgcc tcgttgttaa ctccgccacc
    53221 cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat cgaaattacc
    53281 gttcgtgatg acctttccat gattgccgtg caagggccga atgcgcaggc aaaagctgcc
    53341 acactgttta atgacgccca gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg
    53401 caggcgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg ctatgaaatt
    53461 gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag
    53521 ccatgtggct tgggcgcgcg tgacacgctg cgtctggaag cgggcatgaa tctttatggt
    53581 caggagatgg acgaaactat ttctccttta gccgccaaca tgggctggac tatcgcctgg
    53641 gaaccggcag atcgtgactt tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt
    53701 acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg
    53761 gtacgtttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag cgggactttc
    53821 tccccgacgc tgggttacag cattgcgctg gcacgcgtgc cggaaggtat tggcgaaacg
    53881 gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacgaaacc tgtttttgtg
    53941 cgtaacggta aagccgtcgc gtgatttact tttttggaga ttgattgatg agcaacgtac
    54001 cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc gacggcactt
    54061 tcaccgttgg tataaccgaa catgcccagg agctgttagg cgatatggtg tttgttgacc
    54121 tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa
    54181 aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac
    54241 tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa
    54301 tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca tacgaagcat
    54361 tgttagaaga cgagtaacgg ctttattcct cttctgcggg agaggatcag ggtgaggaaa
    54421 atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt
    54481 caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg cgcttttatt
    54541 gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca
    54601 caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct tgcgaccccg
    54661 ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa ggctattgcc
    54721 agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt gcagctaccg
    54781 ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta cactccgtat
    54841 caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca ggtaacgctg
    54901 gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac cgctgccgcc
    54961 gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg
    55021 gcttccgatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga aacctttggt
    55081 tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg
    55141 ctgttacagc aggttggcac aaccggtgaa atccacgact acaccgcgct tattagcgaa
    55201 ctgaaatcac gcaaaattgt ggtcagtgtc gccgccgata ttatggcgct ggtgctgtta
    55261 actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg
    55321 ccgatgggct acggtggccc acacgcggcc ttctttgcgg cgaaagatga atacaaacgc
    55381 tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc
    55441 atggcgatgc agactcgcga gcaacatatt cgccgtgaga aagcgaactc caacatttgt
    55501 acttcccagg tgctgctggc aaacatcgcc agcctgtatg ccgtttatca cggcccgatt
    55561 ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atatcctggc ggcgggcctg
    55621 caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg tgtggaagtg
    55681 gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat
    55741 attctgaacg cggttgggat cacccttgat gaaacaacca cgcgtgaaaa cgtaatgcag
    55801 cttttcaacg tgctgctggg cgataaccac ggcctggaca tcgacacgct ggacaaagac
    55861 gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga aatcctcacc
    55921 catccggtgt ttaatcgcta ccacagcgaa accgaaatga tgcgctatat gcactcgctg
    55981 gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg caccatgaaa
    56041 ctgaacgccg ccgccgagat gatcccaatc acctggccgg aatttgccga actgcacccg
    56101 ttttgtccgc cggaacaggc tgaaggttat cagcagatga ttgcgcagct ggctgactgg
    56161 ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc
    56221 gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga agggcatcgc
    56281 gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc acatatggca
    56341 ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct gactgatctg
    56401 cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac ttatccttct
    56461 acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc
    56521 ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc
    56581 tttattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat tccgcacggc
    56641 ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggcacc gtttgtaccg
    56701 ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca
    56761 ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat gatgggcgca
    56821 gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat tgccagccgc
    56881 ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt
    56941 attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga tattgccaag
    57001 cgcctgatcg actacggttt ccacgcgccg accatgtcgt tcccggtggc gggtacgctg
    57061 atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg
    57121 ctggctatcc gcgcagaaat cgaccaggtt aaagccggtg tctggccgct ggaagataac
    57181 ccgctggtga acgcgccgca cattcagaac gaactggtcg ccgagtgggc gcatccgtac
    57241 agccgtgagg ttgcagtatt cccggcaggt gtggcagaca aatactggcc gacggtgaaa
    57301 cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa
    57361 taccagtaat tcactgattc gactattttc taaaggcgct tcggcgcctt tttagtcaga
    57421 tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag ggtgagggga
    57481 acaggccggc actggcgcga atatttaccc tcaccccggc cctctccctg aaagggcgag
    57541 ggggaaaagc gtgccaacat tgaagattga gccagtttgt tagcaatctc aaagatacgt
    57601 caacgaatga atttttctcg gaaaaacaaa tggttataga acttgtgacc ggtggcagtc
    57661 gcggcatcgg gcgggcaact gcattactgt tggcgcaaga agggtatacg gtggcggtta
    57721 attatcagca aaacctccac gcggcgcagg aagtgatgaa cttaataacg caagccggtg
    57781 gcaaagcatt cgtgctccag gcggatatca gcgacgaaaa ccaggtcgtt gcgatgttta
    57841 cagcaatcga tcagcacgat gaaccgctag cagcgctggt caataacgcc gggatcttgt
    57901 ttacccagtg caccgttgaa aaccttaccg cagagcgaat caaccgagta ctttccacca
    57961 acgtgacggg atattttctc tgctgccgcg aggcggtaaa acgcatggcg cttaaaaatg
    58021 gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc ctcacggttg ggttcgccag
    58081 gggaatatgt tgattacgcg gcatcgaaag gggcgattga tacgttaacc accggactat
    58141 cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt gcggccaggg tttatttata
    58201 ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga tcgcgttaag tcgaacatcc
    58261 ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc cattgtctgg ctactaagtg
    58321 ataaagcctc ttacgtcacg ggaagtttta tcgatttggc gggcgggaaa taaaacaggg
    58381 aagttgtctg accggatgca acaagtattg catccggtac ttcatcgact taaagcttct
    58441 cgccgttgct ggcaatcacc tcttggtacc agttaaagct cttcttacgt gaacgcgaca
    58501 tatcgccagc accgtcgtca tgtttattca catagataaa gccgtagcgt ttgctgtact
    58561 gcccggtggt gaacgatacg cagtcgatgc agccccacgg tgtgtagccc atcagatcca
    58621 cgccatcgta agtcaccgct tttttcatct cttcgatatg ggcgcgcagg tagtcaatgc
    58681 ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc gtaagcgcca aaaccgtttt
    58741 cgacaataaa cagcggcctc tgataacgct catacagttc gcaaagggca tagcgcagac
    58801 ctactggatc aatctgccag ccccagtcag acgctttaac atacgggttc ggtacgctgc
    58861 cttcgaaacc agagatcgca tcgccggtgc cgccttcggc cttcactgcg ttggtcatgt
    58921 aatagctgaa accaagataa tcgcaggtgc cttcacgcag tacatcgaga tcgccgtctt
    58981 ccattttgat gttaaatccg cgacgctccc actcgttcaa cacataggac gggtaatagc
    59041 cgcgtagctg cacatcggta aagacgtagc gttcgcgcat cgactcctga gcgaacatca
    59101 catcgtccgg gttacagcag tagggataga gcggcaccat cgccagcata cagccgactt
    59161 tcatctccgg gttaatgcga cgcgcagctt tcaccgccag ggcgctggcg acaaactggt
    59221 gatgcagcac ctggtacatc gtctcttccg ggttgtcatg ctcggtataa accacgccgg
    59281 agcagcagta accgaacagc ggtgcacgcc agttacgctg gttgttaatt tcgttgaagg
    59341 tcatccagta tttgacttta tgcttatagc gttcaaacac cacttccgcg aaacggacaa
    59401 agaaatcaac cactttacgg ttggtccagc taccgtattg ctgcaccaga tgcagcggca
    59461 tttcaaagtg ggagagggtg atcaccggtt cgatgttgta tttcagcagt tcatcgaaca
    59521 tatcatcgta gaacttcagc ccttcttcgt ttggctgagc ttcatcacct tttggaaaaa
    59581 tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc catttcggca aatagcttga
    59641 tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg gtttggatag tatttgcctg
    59701 gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc gccaccggtc agaacgtcac
    59761 aaatgctcgg ccctttgccg cctttgttcc agctgccttc gacctgatga gcggcaactg
    59821 cgccgcccca taagaaatct ttcggtaagg tgagtttttt cactatcata ggctccagat
    59881 gagtcaattc ttacaggaaa tagtaccatg cagccaaacg acatcacttt ttttcaacgt
    59941 ttccaggatg acattctggc tgggcgtaaa accatcacca tccgcgacga gtctgaatcg
    60001 cacttcaaaa cgggtgatgt gcttcgtgtc gggcgttttg aagatgacgg ttatttttgc
    60061 acgattgaag tcaccgcaac ctcaaccgta aggctggata cgctgactga aaaacatgca
    60121 gagcaggaaa atatgaccct gactgaactg aaaaaggtca ttgccgacat ctatcccggt
    60181 cagacacagt tttatgtgat tgaatttaaa tgtctttaaa ttgaactcac gcctgttagt
    60241 tgaaatttct catttaattt catgattttt aggagattag tctcaaatca gaatttattt
    60301 tagctaacag gtgttcactg gaactattct cagttacgct ggagaggtat acatgtgcgt
    60361 gtacgtttct ccggagtaag ttatggttca gaagcccctc attaagcagg gatattcgct
    60421 ggcagaggaa attgccaaca gcgtcagtca cggcattggg ttggtgtttg gtatcgttgg
    60481 gctggtgttg ctactggttc aggcggtgga tctcaatgcc agcgccacag cgataaccag
    60541 ctacagcctc tatggcggca gtatgatcct gctgttcctc gcttcgacgc tctatcacgc
    60601 cattcctcat caacgggcaa aaatgtggct gaagaaattt gaccattgcg ctatttacct
    60661 gttgattgcc ggaacctaca cgccgttttt gctggtgggg ctggattctc cgttagcgcg
    60721 cgggttgatg attgttatct ggagcctggc attgctgggg attctgttta aactgaccat
    60781 cgcgcaccga ttcaaaattt tatctctggt gacctatctg gcgatgggct ggctgtcgct
    60841 ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc agcgttacct tactggcggt
    60901 aggcggcgtg gtttattcgc tcggggtgat tttctacgtc tgcaaacgca ttccatacaa
    60961 ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg tgccactttc tggcgatcta
    61021 tttgtatatt gggcaggcgt aatgggtatg cct
//
LOCUS       DAZGCS010000014        60601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGCS010000014 DAZGCS010000000
VERSION     DAZGCS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.014"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..754
                     /locus_tag="ACHK01_000948"
     CDS             38..754
                     /locus_tag="ACHK01_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8095940.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINNQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            784..1284
                     /locus_tag="ACHK01_000949"
     CDS             784..1284
                     /locus_tag="ACHK01_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO8095941.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1376..4060
                     /locus_tag="ACHK01_000950"
     CDS             1376..4060
                     /locus_tag="ACHK01_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO8095942.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4057..5145
                     /locus_tag="ACHK01_000951"
     CDS             4057..5145
                     /locus_tag="ACHK01_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO8095943.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5233..6531)
                     /gene="exuT"
                     /locus_tag="ACHK01_000952"
     CDS             complement(5233..6531)
                     /gene="exuT"
                     /locus_tag="ACHK01_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO8095944.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7014..8426
                     /gene="uxaC"
                     /locus_tag="ACHK01_000953"
     CDS             7014..8426
                     /gene="uxaC"
                     /locus_tag="ACHK01_000953"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO8095945.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8441..9928
                     /gene="uxaA"
                     /locus_tag="ACHK01_000954"
     CDS             8441..9928
                     /gene="uxaA"
                     /locus_tag="ACHK01_000954"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO8095946.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10011..10562
                     /gene="ygjV"
                     /locus_tag="ACHK01_000955"
     CDS             10011..10562
                     /gene="ygjV"
                     /locus_tag="ACHK01_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO8095947.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10567..11811)
                     /gene="sstT"
                     /locus_tag="ACHK01_000956"
     CDS             complement(10567..11811)
                     /gene="sstT"
                     /locus_tag="ACHK01_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO8095948.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12210..13175)
                     /gene="alx"
                     /locus_tag="ACHK01_000957"
     CDS             complement(12210..13175)
                     /gene="alx"
                     /locus_tag="ACHK01_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO8095949.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13458..14444)
                     /gene="ygjR"
                     /locus_tag="ACHK01_000958"
     CDS             complement(13458..14444)
                     /gene="ygjR"
                     /locus_tag="ACHK01_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8095950.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VGAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14523..15206)
                     /gene="ygjQ"
                     /locus_tag="ACHK01_000959"
     CDS             complement(14523..15206)
                     /gene="ygjQ"
                     /locus_tag="ACHK01_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO8095951.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15283..15786)
                     /gene="ygjP"
                     /locus_tag="ACHK01_000960"
     CDS             complement(15283..15786)
                     /gene="ygjP"
                     /locus_tag="ACHK01_000960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO8095952.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15871..17007
                     /gene="rlmG"
                     /locus_tag="ACHK01_000961"
     CDS             15871..17007
                     /gene="rlmG"
                     /locus_tag="ACHK01_000961"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO8095953.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17198..17281
                     /locus_tag="ACHK01_000962"
                     /pseudo
     CDS             17198..17281
                     /locus_tag="ACHK01_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17278..17694
                     /gene="higA"
                     /locus_tag="ACHK01_000963"
     CDS             17278..17694
                     /gene="higA"
                     /locus_tag="ACHK01_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO8095954.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17739..19757)
                     /gene="fadH"
                     /locus_tag="ACHK01_000964"
     CDS             complement(17739..19757)
                     /gene="fadH"
                     /locus_tag="ACHK01_000964"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO8095955.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20083..22434)
                     /gene="ygjK"
                     /locus_tag="ACHK01_000965"
     CDS             complement(20083..22434)
                     /gene="ygjK"
                     /locus_tag="ACHK01_000965"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO8095956.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22451..23521)
                     /gene="ygjJ"
                     /locus_tag="ACHK01_000966"
     CDS             complement(22451..23521)
                     /gene="ygjJ"
                     /locus_tag="ACHK01_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO8095957.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23655..25088)
                     /gene="ygjI"
                     /locus_tag="ACHK01_000967"
     CDS             complement(23655..25088)
                     /gene="ygjI"
                     /locus_tag="ACHK01_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8095958.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25151..25600)
                     /gene="ebgC"
                     /locus_tag="ACHK01_000968"
     CDS             complement(25151..25600)
                     /gene="ebgC"
                     /locus_tag="ACHK01_000968"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO8095959.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25597..28689)
                     /gene="ebgA"
                     /locus_tag="ACHK01_000969"
     CDS             complement(25597..28689)
                     /gene="ebgA"
                     /locus_tag="ACHK01_000969"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO8095960.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRGFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28873..29856)
                     /gene="ebgR"
                     /locus_tag="ACHK01_000970"
     CDS             complement(28873..29856)
                     /gene="ebgR"
                     /locus_tag="ACHK01_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO8095961.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30075..30407
                     /gene="ygjH"
                     /locus_tag="ACHK01_000971"
     CDS             30075..30407
                     /gene="ygjH"
                     /locus_tag="ACHK01_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO8095962.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30449..31828)
                     /gene="ygjG"
                     /locus_tag="ACHK01_000972"
     CDS             complement(30449..31828)
                     /gene="ygjG"
                     /locus_tag="ACHK01_000972"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO8095963.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32246..33766
                     /gene="aer"
                     /locus_tag="ACHK01_000973"
     CDS             32246..33766
                     /gene="aer"
                     /locus_tag="ACHK01_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO8095964.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNSSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33920..34543)
                     /gene="nfeR"
                     /locus_tag="ACHK01_000974"
                     /gene_synonym="yqjI"
     CDS             complement(33920..34543)
                     /gene="nfeR"
                     /locus_tag="ACHK01_000974"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO8095965.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34820..35584
                     /gene="nfeF"
                     /locus_tag="ACHK01_000975"
                     /gene_synonym="yqjH"
     CDS             34820..35584
                     /gene="nfeF"
                     /locus_tag="ACHK01_000975"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO8095966.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35638..35713)
                     /locus_tag="ACHK01_000976"
     tRNA            complement(35638..35713)
                     /locus_tag="ACHK01_000976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35678..35680),aa:Ile,seq:cat)
     gene            35838..36344
                     /gene="mug"
                     /locus_tag="ACHK01_000977"
     CDS             35838..36344
                     /gene="mug"
                     /locus_tag="ACHK01_000977"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO8095967.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36423..38264)
                     /gene="rpoD"
                     /locus_tag="ACHK01_000978"
     CDS             complement(36423..38264)
                     /gene="rpoD"
                     /locus_tag="ACHK01_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO8095968.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38459..40204)
                     /gene="dnaG"
                     /locus_tag="ACHK01_000979"
     CDS             complement(38459..40204)
                     /gene="dnaG"
                     /locus_tag="ACHK01_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO8095969.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40315..40530)
                     /gene="rpsU"
                     /locus_tag="ACHK01_000980"
     CDS             complement(40315..40530)
                     /gene="rpsU"
                     /locus_tag="ACHK01_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO8095970.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40768..41781
                     /gene="tsaD"
                     /locus_tag="ACHK01_000981"
     CDS             40768..41781
                     /gene="tsaD"
                     /locus_tag="ACHK01_000981"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO8095971.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVTAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41824..43287)
                     /gene="ttdT"
                     /locus_tag="ACHK01_000982"
     CDS             complement(41824..43287)
                     /gene="ttdT"
                     /locus_tag="ACHK01_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO8095972.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43336..43941)
                     /gene="ttdB"
                     /locus_tag="ACHK01_000983"
     CDS             complement(43336..43941)
                     /gene="ttdB"
                     /locus_tag="ACHK01_000983"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO8095973.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43938..44849)
                     /gene="ttdA"
                     /locus_tag="ACHK01_000984"
     CDS             complement(43938..44849)
                     /gene="ttdA"
                     /locus_tag="ACHK01_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO8095974.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45056..45988
                     /gene="ttdR"
                     /locus_tag="ACHK01_000985"
                     /gene_synonym="dan"
     CDS             45056..45988
                     /gene="ttdR"
                     /locus_tag="ACHK01_000985"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO8095975.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46001..46618)
                     /gene="plsY"
                     /locus_tag="ACHK01_000986"
     CDS             complement(46001..46618)
                     /gene="plsY"
                     /locus_tag="ACHK01_000986"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO8095976.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46723..47091
                     /gene="folB"
                     /locus_tag="ACHK01_000987"
     CDS             46723..47091
                     /gene="folB"
                     /locus_tag="ACHK01_000987"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO8095977.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47182..48003
                     /gene="bacA"
                     /locus_tag="ACHK01_000988"
     CDS             47182..48003
                     /gene="bacA"
                     /locus_tag="ACHK01_000988"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8095978.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48166..49404)
                     /gene="cca"
                     /locus_tag="ACHK01_000989"
     CDS             complement(48166..49404)
                     /gene="cca"
                     /locus_tag="ACHK01_000989"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO8095979.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49468..50088)
                     /gene="ygiM"
                     /locus_tag="ACHK01_000990"
     CDS             complement(49468..50088)
                     /gene="ygiM"
                     /locus_tag="ACHK01_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO8095980.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50330..51631
                     /gene="ygiF"
                     /locus_tag="ACHK01_000991"
     CDS             50330..51631
                     /gene="ygiF"
                     /locus_tag="ACHK01_000991"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO8095981.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51654..54494
                     /gene="glnE"
                     /locus_tag="ACHK01_000992"
     CDS             51654..54494
                     /gene="glnE"
                     /locus_tag="ACHK01_000992"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO8095982.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54542..55975
                     /gene="hldE"
                     /locus_tag="ACHK01_000993"
     CDS             54542..55975
                     /gene="hldE"
                     /locus_tag="ACHK01_000993"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO8095983.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALVAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56095..56154)
                     /gene="ibsE"
                     /locus_tag="ACHK01_000994"
     CDS             complement(56095..56154)
                     /gene="ibsE"
                     /locus_tag="ACHK01_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO8095984.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56471..56530)
                     /locus_tag="ACHK01_000995"
     CDS             complement(56471..56530)
                     /locus_tag="ACHK01_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8095985.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(56769..58430)
                     /gene="yqiK"
                     /locus_tag="ACHK01_000996"
     CDS             complement(56769..58430)
                     /gene="yqiK"
                     /locus_tag="ACHK01_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO8095986.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(58457..59086)
                     /gene="yqiJ"
                     /locus_tag="ACHK01_000997"
     CDS             complement(58457..59086)
                     /gene="yqiJ"
                     /locus_tag="ACHK01_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO8095987.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59355..59555
                     /gene="glgS"
                     /locus_tag="ACHK01_000998"
     CDS             59355..59555
                     /gene="glgS"
                     /locus_tag="ACHK01_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO8095988.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59598..60599)
                     /gene="yqiI"
                     /locus_tag="ACHK01_000999"
     CDS             complement(59598..60599)
                     /gene="yqiI"
                     /locus_tag="ACHK01_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8095989.1"
                     /translation="MDCYAEHEGGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNVF
                     CNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIAA
                     PECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLP
                     DEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKINS
                     RTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIFH
                     KDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
BASE COUNT        14627 a        15349 c        16052 g        14573 t
ORIGIN      
        1 caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt
       61 atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac
      121 tgtttatcct atggctgttt ccattaataa tcagggtgaa ggcaacgttc gcgtaatatc
      181 taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac
      241 gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc
      301 acctaaattc gctttaccag ccggtagtag caaaaccgta cgttttgttg cgatggaacc
      361 agagcaaaaa gagaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt
      421 tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt
      481 tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt
      541 agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag
      601 caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa
      661 tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact ggatcaccaa
      721 agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa cgaaggaata
      781 gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc
      841 tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat
      901 atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg
      961 cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat
     1021 gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt
     1081 gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt
     1141 accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg
     1201 atgtttgctc aaaccaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc
     1261 atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg
     1321 gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga
     1381 taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac
     1441 caaaattccc gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta
     1501 tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt
     1561 tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc
     1621 gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg
     1681 ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta
     1741 taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga
     1801 tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca
     1861 agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc
     1921 gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc
     1981 agatgatgtc agtgacagta atgctgacgt cagcgatctc tattatcgtt atgatttttt
     2041 acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg
     2101 cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc
     2161 gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct
     2221 ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct
     2281 caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc
     2341 attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac
     2401 cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca
     2461 ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca
     2521 atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa
     2581 taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg
     2641 taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc
     2701 gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga
     2761 gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt
     2821 cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg
     2881 ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat
     2941 ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag
     3001 tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt
     3061 gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc
     3121 aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca
     3181 gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg
     3241 aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg
     3301 aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc
     3361 ctttacgggc acttatagct ctacgctggc tgtcagtcgc tatggcgtta atttgggagc
     3421 cagtggtaca gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca
     3481 ggatgaagat agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca
     3541 gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga
     3601 ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg
     3661 gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt
     3721 taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt
     3781 ggggctgaac agcgccatgt tactggtagc tgaagatggc ggatttacac ttgaaattaa
     3841 cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag
     3901 tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac
     3961 ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg
     4021 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
     4081 atattgggct tgtttggcac gctcactggc gttcaggcag ctcctgacgt gacaagtgaa
     4141 attacgtatg atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg
     4201 gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt
     4261 acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg
     4321 cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg
     4381 ccaggatgca aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca
     4441 tgtacagaag atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt
     4501 gagcagacag agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta
     4561 cattcgaaca gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc
     4621 aaccatattg acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac
     4681 ttgcatccaa caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac
     4741 atgtgcctgt acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat
     4801 gaagggcgac cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg
     4861 accaccaccg acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc
     4921 ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa
     4981 cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc
     5041 ttgactctgg cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc
     5101 aaattgtcag taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac
     5161 aaacgctttt ttctatataa acgacgaagc cgtctgataa ggcggcttcg tcattacaac
     5221 ttaagttaaa cgttaatgtt gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat
     5281 cgccggtttg ttctgcaaca cggtccagat aaccagcgca cctaacaggt caaatactgc
     5341 cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc
     5401 aaacagcgta cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc
     5461 gttacgaccg aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc
     5521 aaaaccaccg atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat
     5581 acccgggcca atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag
     5641 gttcacacca aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag
     5701 gtcagcaaac agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta
     5761 aactttaaac atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgccag
     5821 gaaacgcggc agcgcgatac cccagaactg acggttacgc aggatctgac caacggacat
     5881 tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc
     5941 ttcatcggtc agatgcttct ggtcgcgtgg atgtttatag aaaatcagcc atgccatcgc
     6001 ccagataaag ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcattacgat
     6061 tgcccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     6121 acctaccgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     6181 cgggatcatc gctgcttccg cggcaccgac tgcaccacga gcaatcgcca ggccacccca
     6241 gctaccagcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     6301 accgattttc gtacccagca catccagtac atagcccgct accggttgca tgaccgtata
     6361 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     6421 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     6481 aagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     6541 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     6601 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     6661 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     6721 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     6781 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     6841 aggccagtac tggcgcggcc tgcagagaga tttaccactt tgagagtaat ttttttaact
     6901 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     6961 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     7021 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     7081 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     7141 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     7201 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg
     7261 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     7321 ggacgcacct cgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     7381 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     7441 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     7501 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     7561 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     7621 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     7681 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     7741 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     7801 ctggcgaacc cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     7861 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     7921 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     7981 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     8041 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     8101 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     8161 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     8221 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     8281 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     8341 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     8401 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     8461 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     8521 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     8581 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     8641 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     8701 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     8761 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct
     8821 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     8881 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     8941 acaactgggc gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc
     9001 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     9061 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     9121 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     9181 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     9241 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     9301 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     9361 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     9421 cttcaaacag tactttattg cccatgacca gccgatctat gaaaacccat cgccggggaa
     9481 caaagcgggc ggtatcacca cgctggaaga taaatccctt ggctgtaccc agaaagcggg
     9541 ttccagcgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa
     9601 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     9661 ccatatggtg ctgttcagta ctggtcgtgg cacgccatac ggcggctttg taccgacggt
     9721 gaaaatcgct accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     9781 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     9841 cgttgtgttt gccaacggta aacaaacctg caacgagcgt aacgacttcc gcgaactggc
     9901 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     9961 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
    10021 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
    10081 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
    10141 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
    10201 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
    10261 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
    10321 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
    10381 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
    10441 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
    10501 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
    10561 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
    10621 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
    10681 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
    10741 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
    10801 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
    10861 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgccag ccatattgat
    10921 ggtggcaccc agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca
    10981 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
    11041 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    11101 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    11161 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    11221 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    11281 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    11341 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    11401 aaccatgctc attaccagcc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc
    11461 acccgcgcta ctggataaat gcagggtaga agggaaggca aagctgaaga ctactgcggc
    11521 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    11581 ctgcccgtgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    11641 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    11701 ttttgagatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    11761 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    11821 ctttcattgt gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    11881 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    11941 gattttaaag agaggtcata ttttgcaact taatggttac atttatttga cataaaaata
    12001 aagccaacgt gcggcttcca gactgatgac aacatcaaaa aaggactcgg tcagtcccct
    12061 cgccccgttt ggggagaggg ttagggtgag gggaacaggc cagcatcggt gcgaaaaatt
    12121 accctcaccc cgaccctctc cctggaaggg agagggagaa aacggtgctc atgttaatga
    12181 cagggtacac ggcttaggca gattaaaaat tatccacccc gctgcttatc atgccgataa
    12241 ttcacccagg cgttgataat aaacgtcatc accagaatgc caaacaccac gcccagcgag
    12301 acggcgattg gaatatggta gaagtcgaca atcagcatct tgataccgat aaacaccaga
    12361 atcaccgcca ggccatattt gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa
    12421 tacatcgcac gcaggccgag gatcgcaaac aggtttgagg tcagcacaat gaacgggtca
    12481 gtggtcacgg cgaagatagc cggaatgcta tccacggcga aaatcacgtc gctcaattcc
    12541 accagaatca gtaccagcat cagcggtgtg gcgtacagca atccattctt acgcacaaag
    12601 aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc
    12661 ggcttgtctc caatgcctga ttcatcttca tgggcgagcg ccatcttcac gccggtaaac
    12721 agcaggaagg caccgaagat atacaggatc cagtcgaact gcgaaattag ccagctgcca
    12781 gtgaagatca tgatggtacg cagaacaatc gccccgagca cgccatacac cagcacgcgg
    12841 cgttgtaatg ccgccggaac agagaaatag ctgaacaaca tcagccagac aaagacgtta
    12901 tcgaccgcca gcgatttctc aatcagataa cctgtgagaa aggccagtgc ctgtggatcg
    12961 gcaacggcgc gaccttcggt ttgcaccaga taccaccaga aagcggcgtt aaacagtaac
    13021 gacagcgtca cccagaccag cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc
    13081 ccacgacgcc cctgcaacaa caggtcgata gccagcataa tggcgacaac aacagcgaat
    13141 ccgccccata gcaacggcgt gccgacagta ttcatagaag ttccttacac ataaaaaaac
    13201 ggccaacgtc ggaagacgtc agccgctgct ttttatgcat agacctcgcc ttccggcaag
    13261 gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc
    13321 ggatgtggtt tttcacacat cgtaatgacg atcgaccggc aatgaagtta ctcccctttg
    13381 caggtaacaa agtatgacag accattcagc cagtcaatgg tctgttacat accttttaca
    13441 ctgttttact ttggcaatta tagttttacg ctatctgccg gaaaaatcac cccagtctgg
    13501 cggcggatct cggtcagcag tttggcggtg atgcgactga ccgccagccc cggatgatcc
    13561 accagatgct catccaccag ggtagcgaac agctctgctt cgtagagcat ggtattaata
    13621 tgctgcggct gggtgagatc ctgcatttgg ctgccacgcg gcacgaagca cactttctgg
    13681 cattcagaca gtttttcaat caccagcgat cctgcttcgc cctgaatctc gctcgccagg
    13741 acagaatcac tgacttttga gtgctgcaag gtgacgctga aatcaccgta atccatcacc
    13801 accacgccgt gggcatctac accgcttgcc agcaaactgg cggttgcctg cacgcttttc
    13861 ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa agccgatatc cataattgaa
    13921 ccgttagaga atgacggatt aaaggtgttg ggattttcac cgtccaggta acgttgatag
    13981 cgcgaggaat actggcaata gttgaaaaag actttacgca atttgccgac tttcggcagc
    14041 gcctggcgca gcaaatgaaa gttcggcagg caggcggttt taaatgcctc aaacagcacc
    14101 acctgatttt cccgcgcaca ggcaatggcg gcacccactt ccgccagatt cgacgccagt
    14161 ggtttctcgc aaatcacatg aattttatgg ctaaggaaaa gttgtgtctg ggaaaaatgc
    14221 agggcattcg ggctggcaat atacaccgcg tcaatggcat cgctttccgc catcgcttcc
    14281 agcgaggtaa acagatgctc gacagaaaaa tcattggcga agtgctgagc ctgctcaagg
    14341 ctgcgggaat atacggcggt taacttgtat ttaccgctct catgggcggc ctcgacgaac
    14401 tggcgagtga tccagttcgt accaatcaca gcgaaacgta tcataacgcg tggatactcc
    14461 ataaagggat tctgtcgtca atgtagcacg cctttttcat cttcgacgtg cgcagatacg
    14521 cattattctt ttgccggaca gccatgctca ctaaacggcc caatcatgac cgatggcccg
    14581 agatatttcg gctggcgatg gaggattttg gcgtcaatca cggcgctgac acgagcgagt
    14641 ttctcccgca gttgagtata aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg
    14701 atagcgtcga tgctgtactg ttttgccagc cagatggcgc gttggttatg aaattcttgc
    14761 gagataatgg tgatatggtt ttcaccaaaa acctttttgg cacgcactac cgaatccagc
    14821 gttgagaatc cggcatagtc gcagaagatc actttcgccg gtacgccttt agcgatcaaa
    14881 gcctgctgca tacctgaggc ttcatcgtaa tttttacgtc cgttatcgcc acttaccagt
    14941 agccatttca cttttccggc gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag
    15001 tagcgattac ccggcctggc ccccagcaat aagcccacgt tacgctccgg aactgcgttg
    15061 acatcgctcc aggtcagctg cttgctggcg ttgaccataa cgcgatcggc aataaaaatg
    15121 gtcgctcctg ctataagcaa caacaaacag ccaatcttaa gagtccggcg agaaaaacaa
    15181 atacggagaa gaaggcgggg ctgcatggtc gttatcctta acgaaagggg ggaggtcgat
    15241 tttaggcgct aaagaaagaa aagataacgc ctgacatcat tttcagattt tgtcctgccc
    15301 aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt actgcggctc
    15361 catatggcag cacaactggt aaaacgcttt gttatgctct ttctccttca ggtgcgccag
    15421 ttcgtgcacc acgatcatac gtaaaaacgg ttccggcgcg ttgcgaaaca cagtagcgac
    15481 gcggatctct gcttttgctt tcagcttgcc gccctgcaca cgagaaacag cggtatgtaa
    15541 cccgagcgcg ttttttagca catggatctt attgtcatac atcactttat tgatcggcgg
    15601 ggcattacgc agaaactgat ttttcagatc ctgagtatat tgccagaggg ctttatcggt
    15661 ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac gctgttcgtt
    15721 aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat aagtaagatt
    15781 gttcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt ttcaggggca
    15841 acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg tttccgttca
    15901 ctgacactac aacgttttcc ggcgacggat gacgttaacc cgctacaggc gtgggaagcg
    15961 gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc ggtgttgatc
    16021 ctgaatgatg cctttggtgc gttaagctgc gcgctggcgg aacataagcc gtacagcatt
    16081 ggcgactcat acatcagtga actggcgacg cgcgagaatt tacgcctcaa cgggattgat
    16141 gagtcgagcg tgaagtttct cgacagcacc gccgactacc cgcaacagcc gggtgtggtg
    16201 ctgatcaaag tgccgaaaac actggcattg ctggaacagc aactgcgtgc gctgcgcaaa
    16261 gtggtcacgt cggatacacg tattattgcc ggtgccaaag cccgtgacat tcacacttcc
    16321 acgctggagc tgttcgagaa agtgctcggc ccgaccacca ctacgctggc atggaaaaaa
    16381 gcgcgcctga ttaattgcac tttcaatgaa ccgccgctgg ccgatgcgcc gcagaccgtt
    16441 agctggaagc tggaaggtac tgactggact atccacaacc atgcgaatgt cttctcccgc
    16501 accgggctgg atattggcgc acgcttcttt atgcaacatc tgccagagaa tctcgaaggt
    16561 gagattgtcg atctcggctg tggtaacggc gttattggcc tgacgctgct tgataaaaac
    16621 ccgcaggcga aagtggtgtt tgtcgatgaa tcgccgatgg cggtcgcttc cagccgaatg
    16681 aatgttgaaa ccaacatgcc agaggcgttg gatcgctgcg agtttatgat caacaacgcg
    16741 ctctccggag tggagccttt ccgctttaat gctgtgctct gcaacccgcc gtttcatcag
    16801 caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accacgcccg ccgctgcctg
    16861 aaaatcaacg gcgaactgta tatcgttgcc aaccgtcacc tggattactt ccataaactg
    16921 aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt ggtgctgaaa
    16981 acagtgaagc tggggcgtcg tcggtaagat tgggagttac ctgccggatg cggcgttaac
    17041 gccttatccg gcctacgttc agcacgttac tcccaggccg gataagacgc ggcaagcgtc
    17101 gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt
    17161 caccatcctc tacattctct tgtttagcgt tgttcaacgt ttatgctaca tacgtgaagt
    17221 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    17281 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggc accttttctg
    17341 gcgggtattc agaacgagga acaatacgcc caggcgctgg aactggtaga tcatctgctg
    17401 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    17461 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    17521 gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt
    17581 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    17641 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    17701 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    17761 gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt
    17821 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    17881 cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca
    17941 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    18001 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    18061 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    18121 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    18181 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    18241 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    18301 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg
    18361 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    18421 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg
    18481 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg
    18541 tgggcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    18601 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc
    18661 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    18721 atctgatcga ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc
    18781 gattgcgctt ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgacaccata
    18841 tcggcatcgc cgcgcgagag aatgtcgtcg gcaacctgcg gatcgttaat ccggttggtg
    18901 gtcaccagcg gcagcgaaac gtggcctttc agtttgcgcg tgacccaagt aaatgcgccg
    18961 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    19021 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    19081 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    19141 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcattcggtt gcggtaatcg
    19201 ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac
    19261 ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag
    19321 tgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    19381 cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc
    19441 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg
    19501 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    19561 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    19621 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    19681 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    19741 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    19801 gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    19861 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgcgt tttatccggc
    19921 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcacgg
    19981 tgcagatgcc ggatgcgacg cttgtcgcgt tttatccggc caggaggatt gcacatgtag
    20041 ggcggataag gcgtttacgc cgcatccgcc agcagttgca aattattgct tacggaaaaa
    20101 atcgttatac aacatataca aatgcgcggc actccaggag aaatttggtg cgccttgctg
    20161 tgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc
    20221 gtgacggaag aacgtatccg ccagtttcag ggcatcatcg cgataaccgt aacgctccat
    20281 ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccacgccagt agatatcagc
    20341 gccaaaagcc ggattggtta acgccgccgt tcccagcggg acaaaggtgt tgaactcttt
    20401 aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa
    20461 cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc
    20521 gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag tcgggtcgaa
    20581 catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc
    20641 ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc tgtacatata
    20701 gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc cgtcctgact
    20761 gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag cgacatattt
    20821 atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacgcc ccgattccca
    20881 cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct ccaccacgcg
    20941 ggcgtagttg ttcagcccag actgcgtctc ttctttgttg cctttcttca ccgtaaacag
    21001 catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc
    21061 gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta
    21121 catctctgcc aaccaggctt tatcctgggt gacgttgtac acttccatca ccgaccaggc
    21181 ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac cacgctcggg
    21241 gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgctatc
    21301 gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga tgtcgggatt
    21361 gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct gattgccgga
    21421 gaaccagcgc ccggtcaccg acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga
    21481 gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt
    21541 cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct gcgaggcggt
    21601 gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc
    21661 ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc
    21721 cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg
    21781 atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac
    21841 tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt cgccagcaat
    21901 ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccgtc
    21961 ccacaccaga tccagcggtt tattgctggt gattttggtt tccagtagtg acgtgcgcgg
    22021 cgtggcgaag cgcagagtca tttcgacttg cacatctttt gctgtcagtt tttgcaccag
    22081 cgcaccggga atactgtatg cctccagcgt aaagtcgact ttcttgccgt cctgccagac
    22141 ggtcaggcgg tcgaaattgc tggccataaa gttgatgtac tcttccgtca gcagcgcaac
    22201 gcccggaaag ccgcccatgg tgttagggcc gtctggcaac agatgaccat gccaggctcc
    22261 gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg
    22321 cggcgcgcca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgggcgga
    22381 aaaacttatc agcagagcgc aggttactgg cgttaaaata gtttttattt tcatgaaatt
    22441 acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgtgag
    22501 aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt
    22561 cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat
    22621 gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc
    22681 tcaccatcat cccactttca cttcccggta cgtccagccc gtcggcgcgt ccggtatggt
    22741 gccaggcgcg agcggcaaag ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc
    22801 cgtcggtgat ttccagaccg ttcatccagg tgatgccttc ggcaaggtca tactggatgt
    22861 aaccgttcat cattgccgga taggtgtatt tgtcgtcata gcgatcgtac ttgccaaaat
    22921 gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa
    22981 agttgccgct taagttctgc accttcagcg cggtcataat gtcgcgggta ttgggaatga
    23041 cgtagcccag atcgggggtg aagtcgcccc accattgcag gtcatccagc gaggaatcct
    23101 gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt
    23161 taattccgcc ctcgaaacca ccccagctat ggtctggcac ccaggcattg ccgtcgtggt
    23221 cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct
    23281 ccaggccacc ttcaatatag gtaccgtcgc tgtatttatg tttattctca ccctctaaat
    23341 ccatatatcc accaacccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg
    23401 taattgcagg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag
    23461 aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca
    23521 ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat
    23581 ataaaggcag aaactatttt ccccctcccc atccaggaag agggaaaatg cctgctcatt
    23641 ttttattagt ctgctcaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca
    23701 gccgttaatc cctttatata tttactgtat ttccaccacg caaagccgag gaagataaca
    23761 ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa
    23821 gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg
    23881 cgggagccca tgcggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa
    23941 gccagcatga tgaataacgg cggaagcatg gacgctgcgg cagtcatatt aataatagta
    24001 ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag cgggatgacg
    24061 atcaggaact ggatccacgc tgcgcgcgct ggaacgccgt tttcgttcag ttcgacggtt
    24121 ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc
    24181 cacatcagca gggaaccgaa catcgcggta aaggacacca gaccgacaaa gcgattcatc
    24241 aacgcttccg gtagaccaaa atacgccgcc atgccgtgga atacctgcac cgatccgccg
    24301 gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga gacggaatac
    24361 agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt cgaaccgcct
    24421 ttgacgtcgt taacgtacac agcgacggac tccgcgccac ctgcggccat aaagatccag
    24481 gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac ggtgatgggg
    24541 tcagcaggct gtacgccgcc aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt
    24601 aacagcagca tcagcgttga agtgacggag gtaattggcc ccaacatttt cgccccgttg
    24661 gtggaaaccc aggtggagaa ggcgaacagc accatactga taatggtggt ggcaaccggc
    24721 gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac gcgcggcaac
    24781 agtgaggtga aaaagaacag gtttacgaac cagtaggtat aggcagtaat aaatgcccaa
    24841 cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt tttatttaac
    24901 gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa
    24961 aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt gttaaagctg
    25021 taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt acgtttggta
    25081 tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc
    25141 gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg agaaccactt
    25201 ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa ataacgattt
    25261 gcccttcgtg gacctcaacg gtttctccgc agccttttaa atattcacgg tcagtttcat
    25321 cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc
    25381 cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg
    25441 cggagtctgt ctccacgcgg taagtcaatg agtcaccgat ggagtgggcg acgccaggct
    25501 gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt
    25561 aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct cgctgtgcaa
    25621 attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg cggtagcttc
    25681 tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc agacgcgcca
    25741 ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagcagct ggtggtcgag
    25801 gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt
    25861 ttcctgggta tagcgccagg cgctgaagtt aattgggcgc tgcggaacca ccagcagacc
    25921 gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc cgttgttctg
    25981 cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat cgatgatgtt
    26041 agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg ccacctgatc
    26101 gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc
    26161 gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc gccagatgta
    26221 ggtgcagcgc atcccgaagt caaacaccgg cggggcaata actgtgcggc tgatgatcaa
    26281 cacttcacca tcgctctgtt ctacggcaaa gccgcgcaga tgttcctgca tgatctgcaa
    26341 atgattcggt tgccacagcc cttcgtactc ctgcttgtgg ttgtcgatca ttggcttgaa
    26401 gaagttgatc tttggctcgc gggtcagcag cgactcgcca ttcacctgcc aggatgtcgg
    26461 tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa
    26521 acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca ctggttgcgc
    26581 ggtgttttcc ttcagcggga actgataagt ggcgatagaa tgcccggctt cgctgtagcg
    26641 ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg
    26701 cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg
    26761 cgtcgcgagc gtttcacctt cgacgcgcac ctctgcgtgc agggtgtagt catcaagcgt
    26821 ggtaaaccac agtttatttt cgactttcag ctcgccgtga gtcagatcca gcgcgtggat
    26881 ttttaccggc gcgataacct gtttgtactc tttcaggccc ggtcccggcg tctgatcgga
    26941 atagatcaaa ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc
    27001 gaatttatac cagacgttgc cgttgtcatc ctgcgcctgg atcccgtggt cgcaccattc
    27061 ccagacataa tgtccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag
    27121 cccgcctggt ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg
    27181 cgggtattca ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc
    27241 gaccacttca gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt
    27301 cgctgcgtgg tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca
    27361 gatgatgatc gacggatggt ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta
    27421 gactttttcc cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc
    27481 gtgcgattcg acgtcggttt ccgccatcac aaacaggccg tagatatcac acagttcgta
    27541 aaaacgcgga tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat
    27601 caactggaga tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt
    27661 gtcgtgacgg ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc
    27721 gcgcactttg atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt
    27781 ggcgtctttc agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg
    27841 cggctgttcg acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc
    27901 aatggcgctg ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac
    27961 gacaggggag gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata
    28021 ggcttcgtca aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac
    28081 cagataaaca tcgcggaaga tccccgctga ccaccacata tcctggtctt ccacgtaggt
    28141 agagtccgcc cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc
    28201 gctgatgtca aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt
    28261 aacatagact tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg
    28321 ccagccgtcg ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac
    28381 aaacggcaca tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc
    28441 ttccatttgc cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt
    28501 gaaggcttct ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa
    28561 gggcagaaac aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata
    28621 tgaaaaaaag tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca
    28681 gcgattcata gggcctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg
    28741 tgtaattgct ggggagttta gtaaaatttt tactaaaaag tagcaggtaa atgcatttac
    28801 tttaactgga tcacgaaaat aaacggcagc actgtccggc aaaaacgatt gccggacagt
    28861 gtgtaagggg atttaacggg tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa
    28921 cagcggcagc gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc cctgacttcc
    28981 catcatctcg gaatggatgc gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg
    29041 gatatcgtta acgctgataa gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat
    29101 tgcccgcagt acgccgatag caatggaatc ggaagcaaca aacagtgcct tcggatagtc
    29161 ttcccgcgcc agcatttgtt ttgccagttc ataacccgac gaactggaaa aaccgccgcg
    29221 ccagatatcc tcttcgcgta ccacttgttt cagtcggcca tattccgcaa aggcgacctc
    29281 acgaatatcc gccttgccag gctcatcttc accgccaata aaaccaatac gattaacgcc
    29341 ctggttgata tagaagtcga tgatttcttt actgatgcgt gccagatcga tatccaccgc
    29401 atcgtaaccg ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg tcaacgcaca
    29461 ggcggcggcg cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg tgacgttttt
    29521 aatgtctggt aatccgctgt gttcataaca gttggtgagc tcaatgccca gcttttcgca
    29581 ctgagtttca atgccgtggc ggatcgccag atagtaagga tcgttgatct ccagctcctg
    29641 ttggtagctg tagatagcca gaatatggtg ttggttgact gcacctgtct ggagtttacg
    29701 ggcactactg gtcttgtact ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc
    29761 ttctttcaca ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg ccagggatac
    29821 gccagcttcg attgcgatgt cttttagtgt tgccatgttt acctttttta ctttccctga
    29881 tgtgcgcccc cattgtagag gaagtgctta tcgggtcacc tgttttcagt aaataaagcg
    29941 cgtttctttg acgctgtaac ggtaacacag cggctcacga atgtgatcac gcctgcattc
    30001 tccattttta gcctacagga ttgagaaaaa cttttgctat agtttagtaa aattttaccc
    30061 gaagggagga aacaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    30121 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    30181 ttgatgtggg ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag
    30241 aagagctgat ggggaaaacg gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg
    30301 gtgaaacgtc ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgt
    30361 tgttaacgcc ggagcggatg atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg
    30421 cgacgtcgta tcgccatccg atttgatatt acgcttcttc gacacttact cgcatggccg
    30481 ccagcgcctt acgcgccgct ttgatcacca gttcacactg ttcaatggtc agtgtcagtg
    30541 gcggttcaat gcggatcgtt ttggcgttat tgagcgttcc ggccaccagt acgcgctggc
    30601 ggaacatctc gctggcaaag ttatagccga tttcgttatc aacaaactca attgccatca
    30661 acatcccttt accacgcgct tcctgtacca gatcgggata ttcccgcgcc agttgacgga
    30721 aaccgtccag caacatatcg cctttttgct cagcctgagc cggtaagttc tgctccagca
    30781 acacattgat ggtcgccagc gccgccgcac aggccagcgg gttgccgcca aaggtggtgg
    30841 tatgcaggaa tgggttgtcg aacagaactg aaaacacctc ttcagtggcg atggtcgcgc
    30901 caatcggcat cacgccgccg ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt
    30961 tctcatgctc gcaggcgaac atcttgcccg tgcgccccat gcccgtttgt acttcatcga
    31021 ggatcatcag tgcgccgaac tcatcgcata gcttacgtac ggcggtgaga tagcccggcg
    31081 gcggcagaat tacgccacct tcaccctgaa tcggttcgag gatcaccgca gccacatcat
    31141 caccggtttt tttgcactcg ttaagagccg tgcgcatggc ttcgatattg ccaaacggca
    31201 catgacggaa gcccggcagc agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg
    31261 ccgacagcgc gccaagtgat ttaccgtgga acgcgccgct ggtggcaata aaagtaaact
    31321 tgccgcgcgg tgactggtaa gctttcgcca gcttcagcgc tgcttcaacg gactcggtgc
    31381 cgctattaca gaagaagctg tatttcagtt taccgggcgt tagcgcagca agggttttcg
    31441 ccaacatcgc ccgtaacgga tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt
    31501 gattctgtac ggcggaaacc acaactggat tacggtgccc cacgttgaaa attccaaaac
    31561 ctcccaggca gtcgataaac tcctgtccct gggtgtcgac aagcgtattt aaacctcccg
    31621 cttgccactc tacggctccg taatccccgc cggcggtaac agatttgcga tactctaaaa
    31681 accccggatt gacatgctct ttgaagtatt caatcacctc tcggttaagt gctttcatct
    31741 cctcatgatc cagcgttcgc ttctcaatga gattcagggc gtgggcgctg cacgctaaag
    31801 ccgatgcgct cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat
    31861 accgatttaa gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa atcgggataa
    31921 acacaaattc ggttatcatt tgtgcaatat ttaatgctat ctgttaacat ttgttgttat
    31981 tttgcacggt ttttgtcggg tatggatttg atgattgctg cgcaaatgca cgttttcagg
    32041 gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta acagagtgaa
    32101 agtattttaa atcaaagaat tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa
    32161 ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga agttaacaac
    32221 catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac
    32281 cccgctggcg gacgatacca ctctgatgtc cactaccgat ctgcaaagct atatcactca
    32341 tgctaatgac acttttgtgc aggtgagcgg ctttaccttg caagagttac aagggcagcc
    32401 gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata tgtggttcac
    32461 cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa atggtgacca
    32521 ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat
    32581 gtcgattcgt acccgggcga cggatgaaga gattgcggcg gtggagccgc tgtacaaagc
    32641 gctgaacgcc ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg
    32701 gctgggtaaa ctgccttcat taccgcttcg ctggcgggcg cgtggagtga tgaccctgat
    32761 gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga cgtatttcct
    32821 ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat
    32881 agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata gtgttgagca
    32941 cctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc ttggcctgat
    33001 gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa atagcagtga
    33061 gacgctggcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag ttgataacgt
    33121 tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac
    33181 ggcgtcggct gccgataaac tttctatcac cgccagtaat gcggcagtgc agggtgggga
    33241 ggcgatgacc acggtgatca agacaatgga cgatatcgcc gacagtaccc agcgcattgg
    33301 caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg ccctgaatgc
    33361 ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga
    33421 agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac gatattcgca agctgattga
    33481 tgccagtgct gataaggtgc aatccggttc gcagcaggta cacgccgccg gacggacgat
    33541 ggaagatatt gtggcacagg tgaaaaacgt cacccagttg atcgcccaga ttagccattc
    33601 aacgctggaa caggccgatg ggctttccag cctgacccgt gcagtggatg agcttaacct
    33661 gatcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg cgatggtgaa
    33721 acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg tatgccggat
    33781 ccggcggttg gagcacaatg cctgatgcga tgctggcgca tcttatcagg cctgcggggt
    33841 gtgtagcggg tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg
    33901 ccactggcgg gtgcggcgat taatccagtt gcgtaatatc aaaagcggcg cggtcgatca
    33961 ccgcaatgat cttttttatt tgtgcatcac tgatatcgct ctggttgacg tgtaaatcca
    34021 gcaccgcttt aaaattatcc agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc
    34081 caacgcaacg cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca
    34141 gccactgagc gccttgttcg gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga
    34201 tggtaatcag cgactgctcc tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg
    34261 gggtgtaatt cccctgggtt aggttctcaa tcgctttaat caattcgtaa ccgtggctgt
    34321 catcgcgcga gagaatatcc agaatcacca gacgcaattc accgtgacca aagaaacgtt
    34381 gccgacgacc gccgccgcga ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt
    34441 gaccgtgtcc gcagtgctcg tgttctgact tctcaccttt gcagcagccc tcatggcgtg
    34501 gctggccttc atgtttacaa cacccttcgt gatgatggct catatcagcc tccttttgct
    34561 tgattagata tatctaattt atatctgatg tgagcattac tgcaagtaca aaaatgatgt
    34621 ttaaacgtgg aagcacctca catccaggcc ggataagacg ttcacgccgc atccggcagc
    34681 attgctatgg cactgaatgc cttcacatac ttcagatata tcagttctac aaatcgcttg
    34741 catttatcat gattaacaat cattatcatt tacgaaaatt atttagatat atctgattac
    34801 tatcacgaag gcgataacaa tgaataactc cccccgctac ccgcagcgcg ttcgcaatga
    34861 tctgcgcttc cgtgaactga ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg
    34921 cattgtcctc ggcggcgagg cgctggacgg ttttacatcg cgcggctttg acgatcacag
    34981 caaactcttc tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg
    35041 catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga
    35101 actacgccat gaactggcga tcgatttctt tattcacgac ggtggggtcg ccagcggctg
    35161 ggcgatgcag gcgcaaccgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt
    35221 ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact
    35281 gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt
    35341 tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga
    35401 atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc
    35461 cgatgattac ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg
    35521 ctttgaagcg gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa
    35581 gtaactgata tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg
    35641 tggcccctgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact
    35701 gagctaaggg gccatggtag cggattataa agtaactccg tgtcgcaatc cagccattac
    35761 cgtgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat
    35821 gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt
    35881 gcggtatcaa ccctgggctt tcatccgctg ggactggttt tccctttgct catccagcaa
    35941 atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg
    36001 aggcacagca tctgctggat tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg
    36061 tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa
    36121 aaattgaaga ttatcagccg caggcgttgg cgattctggg caaacaagca tatgaacagg
    36181 gattcagcca gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga
    36241 tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actagagaaa ctggttgaag
    36301 cgtatcgcga gctggaccag gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa
    36361 ggtggcgttt gtgcaaaggg actagtgccg ggtgcggcgt aacgcctgat ccggcctacc
    36421 gattaatcgt ccaggaagct acgcaacact tcagaacggc tcgggtgacg cagtttgcgc
    36481 agcgccttcg cttcgatctg acggatacgt tcgcgggtaa cgtcgaactg tttacccact
    36541 tcttccagcg tgtggtcggt gttcatatcg ataccgaaac gcatacgcag aactttcgct
    36601 tcacgcgcgg tcaggccagc cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc
    36661 gcagaatcca gcggcagctc gagggtggta tcctcgatga aatcccccag atgcgaatct
    36721 tcatcatcac cgatcggcgt ttccatggag attggctctt tggcgatctt cagcactttg
    36781 cggatcttgt cttccggcat cagcatacgt tcagccagtt cttccggcgt cggttcacgg
    36841 cccatctctt gcagcatctg gcgagaaata cggttgagtt tgttgatagt ctcaatcata
    36901 tgcaccggaa tacggatggt gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg
    36961 atccaccagg ttgcgtaggt ggagaacttg taaccacggc ggtattcgaa tttatcaacc
    37021 gctttcatca gaccgatgtt gccttcctga atcaggtcaa ggaactgcaa gccacggttg
    37081 gtgtatttct tggcgataga aataaccaga cgtaagttcg cttcaaccat ctctttcttc
    37141 gcacggcggg ctttcgcttc accgatggac atacgacggt tgatatcttt aacctgctcg
    37201 atggtcaggc cggtttcttc ttcaatctgc tgcagtttct gcagggcgcg atgcacttct
    37261 tcagagacat cgtgcagttt ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac
    37321 caggtatcgc tggtttcgtt gccggtaaac agggtaatga agtttttctt cggcattttg
    37381 cactgctcaa cgcagagctt catgatcaga cgttcttgcg tacgaacgcg gtccatcatg
    37441 acgcgcatgc tgttgaccag gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat
    37501 acttcagaca gtttcaggat ctcttcctga gcggcagcgt gactgcgacc tttcgctttg
    37561 atggtgtcac gcgttacaac gtactgagcg cgtagttccg caaatttttc gcgagccagt
    37621 tccgggtcga tgctgttgtc atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca
    37681 tcttcgtcat cgtccagatc ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt
    37741 gccagatctt cttctgcgtt cgggtcaaca aagccggtga tcagatcgga cagacgcgct
    37801 tcttctgctt caacacgatc gtactgttcc agcagatagg tgatcgcttc cggatattca
    37861 gcaacggagc attgaacctg gttgatcccg tcttcaatac gcttagcgat gtcaatttcg
    37921 ccttcgcggg tcaacagttc aacggtgccc atttcacgca tgtacatgcg taccgggtca
    37981 gtcgtgcgcc cgatttcaga ttccacgctg gaaagcacct gcgcggcggc ttcggcagca
    38041 tcttcgtccg cggtgttttc agccagcatc agatcatcgg catccggtgc ttcttccatc
    38101 acctgaatgc ccatgtcgtt gatcatttgg atgatgtctt cgatctgatc tgaatcgacg
    38161 atatcttccg gcagatggtc attgacctcg gcataggtca gatagccttg ctccttacca
    38221 cgggtgacaa gaagtttcag ctgtgactgc gggttttgct ccataagacg gtatccacac
    38281 ttatttcatg aggttggtgt tgggcgattg acccgatgtc cttcagccgt taacgctgaa
    38341 atccaacggt tgtttaccgc caacgataat tacgagggcg tacttatata tttgccgctg
    38401 cctctcagtg cggctgtcgg gggcttcccg atcgctcttc ggcacttaag ccgttaaatc
    38461 actttttcgc cagctcctgg tttaatgtcc agagctccag gcgttcttcg ttgcttaaac
    38521 catgcgtgcg ctcacgagcg attaactctt cctggcgcag ttcaagcagc gaatcaaaca
    38581 tatggttgag tgagtcggtg aaggtttgct cagcaatatt cttatctgct atatcgtccc
    38641 acatcgacag tttttcaagg gtggcagcat tatttgtacc acgatagtgc tctaaaagtt
    38701 gcccggtggt cagacctggc tgggagagac aagtgttgac cagttctctg aataagccaa
    38761 gtccagggag cttattttca tccagattct caagcggcgg gaccaacgtc gctaattctg
    38821 gattttgcac cagcaaccct ataagtatac gcatggtcgt gcgttttagc tgcggaacag
    38881 ggcgagaaac gccgctctct gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa
    38941 gtatgcctaa tttgttgcct aattcctgac gaagatatat tcgcagcgtt tcgcccggca
    39001 cttgcgatat caatggcagt gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca
    39061 gatcaacttg cggcatcagg ctgttaaaca gaaatgcgga aagcggcatc gcctgctcca
    39121 tccgcgcttc aaacgcttct ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat
    39181 ccggcaaaaa cataaagcgt agctgacggc cgtctgtcat gtaaggcagc gccgtttcca
    39241 gcgctcgcca tgcggcatcg cggcctgcac ggtcgccgtc ataacagcaa atgacattgt
    39301 tggtcgcgcg gaacaacagt tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa
    39361 cggcgtaatt aatgccgtat tgcgccagcg ccaccacgtc catatagcct tcgaccacaa
    39421 gcagacgatt gggttcagcg ttatcctgct gcgcttcata aagaccgtaa agctggcggc
    39481 ctttatggaa aatgtctgtt tccggcgagt tcaggtattt gggggtatcg ttgcccagca
    39541 cgcgcccgcc aaaaccaatc acccgaccgc gtttatcgcg aatggggaac atcacccgct
    39601 cgcggaaacg atcgtaactg cgtccctgat cgttagtgac caacatcccc gcatcaatca
    39661 atgactggcg attttctgga ttgccgccaa accgcttcag gacgttgtcc cagccggggg
    39721 gcgcaaaacc aatcgcaaag cgagcgataa cctcgtggct taatccgcgt ttttccagat
    39781 actggcgcgc agacgtggca acaggttgtt gtaaagattg ttggtaaaac gtattcagac
    39841 cgtccatcaa ctgataaagc gtttgcctct gatggcgctc gatctggctg gggccgctgc
    39901 ctgcttcaaa tggcacttca agattgtgca ttgctgccag ctcttcgacc gtttcaacga
    39961 actcgagctt gtcgtagttc atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa
    40021 agcagtggta aaactgtttc tcaccgttaa cggtgaatga cggggttttc tcgttgtgga
    40081 atggacaaca cgcgtggaaa ttcttgccct gctttttcag cttcacacgg gcatcgatca
    40141 gatcgacgat gtcagtgcgt gccagcagat cattaatgaa tacgcgtggg attcgtccag
    40201 ccataggccc cgatttttag caattcataa acgaaaataa gccgcgcatt cctttcggaa
    40261 gcacggcctt acaactacaa ctcggtctga tcggagagca acgctctcgg ggaattagta
    40321 cagacgagtg cggcgtgcgt tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga
    40381 agctttagcg cgcttacgtt cggtagtcgg tttttcatag aactcacgac gacgaacttc
    40441 cgccagaaca cctgcttttt cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg
    40501 ctcgttttca cgtactttaa ttaccggcat gtgcctctca cctttgatta attcggtttg
    40561 ccgctggcat caacgccagc ttatttcaaa atggtgcgga attttactgc aattgctgct
    40621 gctttgtaaa gcaccgcggc cttttttgaa agggactttt ataagggtga ggagtataca
    40681 cgaggctttc tccaggggcg aacaaagttt tacatcaacc cgcattggtc ctacactgcg
    40741 cggtaataaa gcgaggtaaa acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat
    40801 gaaaccggca tcgccattta cgacgatgaa aaaggtttgt tagccaacca attgtatagt
    40861 caggtgaaat tgcacgctga ctacggcggc gtcgtgcctg aactggcctc ccgcgatcac
    40921 gtgcgcaaaa ccgtaccgtt gatccaggcg gcgctaaagg agtctggttt aacggcaaaa
    40981 gacattgatg ctgtggccta taccgcaggc cctggattag tcggcgcact gctggttggc
    41041 gcaaccgtgg ggcgttctct ggcgtttgcc tggaacgtta cggcaatccc ggtacaccat
    41101 atggaagggc atctgttagc gccgatgctg gaagataacc cgccggaatt tccgtttgtc
    41161 gcgctgctgg tgtccggcgg tcatacgcag ttaatcagcg tgactggcat tggtcagtac
    41221 gagctgctcg gcgagtctat cgatgatgcc gccggtgaag cgtttgataa aaccgcgaag
    41281 ctgctggggc tggattatcc tggcggaccg ttactgtcga aaatggcggc tcagggtact
    41341 gccgggcgct ttgttttccc gcgtccgatg accgaccgtc cggggctgga tttcagcttt
    41401 tctggtctga aaacctttgc ggcgaacacg attcgtgaca acggcaccga cgaccagacg
    41461 cgtgctgata tcgcccgcgc ctttgaagat gcggtggtcg atacgttgat gattaagtgt
    41521 aagcgagcgt tggatcagac gggctttaag cgactggtca tggcgggcgg cgtgagtgct
    41581 aaccgcacgt tacgggcgaa gctggcggaa atgatgaaaa aacgccgcgg cgaagtgttc
    41641 tacgcgcgtc cggaattttg tactgataac ggcgcgatga tcgcctatgc cggaatggtg
    41701 cggtttaaag caggcgcgac ggcggatctc ggcgttagcg tgcgtccgcg ctggccactg
    41761 gcagagttac cggctgcgta aaactatccg gtgccggaga gcaatttccg gcaccgtcct
    41821 cacttaaagc aacaccacgg gcatccacag caagccggta atcaccagca acacgaggaa
    41881 tatcagtcca aaaatcgccc ccagtcgcca gtaatccacc gttggcagat aaccactacc
    41941 gtagtaaatc ggactgggtc cggtggcgta tggtgtaaga atgctcccca gaccaattgc
    42001 cgcgccaacc atcaggcaga acaccggcag cgggatttcc ggcatcgcca gcgcagcggc
    42061 aatcatcatc ggtgcgaggg cggaggtata cgccgtggcg ctggcgaaaa agtagcgcag
    42121 cagataaaac accacaatca acgccaccat caccatcgtt ggcgaataac cgcttaagct
    42181 gcctgctaac agtttgccaa accagctaat aaaaccagtg ttgttgagtc cggtagcgag
    42241 ggtgataagc gaggccagcc agaagaaaac gttccacgcg gctttattac tgacaatgtc
    42301 gtcccaactg ataatgcgca gaagcagcat cagtgccacc acgctgtaac cgaccatcgc
    42361 ggcatcgata taatcaccgc cgaaaatcca cagcaccagc gcgcctacca tcagccccag
    42421 catccgtttt tcacgcgaac agagcgggcc cattgcctgc agttccgtct ctgcccagcg
    42481 cggcacctga tcgcctgact tcagtaccgg cgggtacagc acataagcca gccagggaac
    42541 cagcagcacc agcaaaatac tgagcggcaa catcccgagg aaccagtcgc cccaactcag
    42601 cgtggcgtga gatgcgcttt tcatcagtcc aattaacagc aagtttggtg ccatcgccgt
    42661 caagaaaatg gcgctggtca cgcagtcggc aacaatcccc atccacatga tgtacgagcc
    42721 aatgctgcgc gaactgctgt cgtttggttg tgattgatag agcggtggca ggttacggat
    42781 gatgggatag ataatccccg caccacgcgc cgagttggac ggtgttacag gtgcgaggat
    42841 cagctcggag aacatcaccg catagccgag aaacagcgtg cgatgtccca tctttttcac
    42901 cagaatcagc gcaatgcggc gtcccagccc ggttttttca tagcctgtgc caaacataaa
    42961 ggcggcgaaa atcagccaga taaccgaatt agaaaaaccg gaaaccgccc acgagaggga
    43021 ttttgcagtg aatttaaagc ctggctgggc gagctgttcg gggctgaaca gcagccaggg
    43081 agagagtatg gcgatgatgg agatacccac catcgccacc acggcacccg gcacgggttc
    43141 gaggatcagt ccaacgatca cgccagtaaa aacggcaaag tagagccagg tatgactctc
    43201 cagccctgcg ggaaccggaa ttagagcaat aatggcgatg accgccagcg gcgcaaggta
    43261 tcgccaccat tcagtggaag gtttcatcgt gttatgcctg cgaaaaagga gagggggcga
    43321 gcctctccgg gagggttatt tgatgtaatg gacgtgctcg cagatctctt ccacgatggg
    43381 atcgcggcgt tcggcgaaca gctttttgtt ttcggctatc aggttgttgc cgtgggtatc
    43441 aatagagaca atcagcgggc cgaactcttt gacccggcag acccacagtg actccggcat
    43501 cccgagttct gtccagtgca cttcttcaat ctcttccacc tgggttgccg ccagcaccgc
    43561 gcagcctgcc gggaaaatca catgtagcgc cttgaatttc tggcagcctt cttcggtcag
    43621 cggccccatt ccgcctttgc caaccaccag tttcacgccg gtctgctcga taaattcacg
    43681 ttcaaaactt tccatacgca tactggttgt cgggccaacg gaaaccatct cccatttatc
    43741 gccgtttttg cgcacgatgg ggccagcgtg gaaaatcgct ttgccgttga gatcgtaagg
    43801 gatcggacgt ttcagttcga tcaagcggcg gtgacaaacg tcacggcagg tcaccagcgt
    43861 accggtcagg tagatcacat cgccgacgcg aatatcttgc agatcttcag ctttgatcgg
    43921 ggttgtcagg atctttttca taacgcgctc cgggtgtgag acagattttc aaaggtgaga
    43981 tcggcatgaa ccagcagtgt gccgcgacga tgcgcccagc agccggtaga gacagcaacg
    44041 ccgatggttg acggatggcg ggcggcagat tcgatatgca cgcccatcac tgaactgttg
    44101 ccggtcagcc cttgtggacc aatccccaga cggttgagtc cttcttccag gcgtagctcc
    44161 agctctgccg cttttggatt gggatggcgg ctgccaatcg gacgcaaaat ggctttacgc
    44221 gagagtacgg cggcggtttc caccgaggtg gcgatgccca cgcccaccag taccggtgga
    44281 caggcgttta cggcgagggt ggagatattt tcgaagacga atttcaccac gccttcgtag
    44341 ccttctgacg gcattaacac tttcgagcgg ccaggtagcg tgcagccgcc gcctgccatg
    44401 taaacttcga tttctgcatc gtcattgtcg gggatgatgt cccaggtgac ccacggtacg
    44461 ccgctaccgg tatttttgcc ggtgtttact tcgtcaaaaa tttctaccgc attgtgacgt
    44521 agtggcgctt tgacggttgc ctcttccacg gcttgtttga gtatgctttg cagctcgcca
    44581 agcagtggga agcgggaacc gactttaacg aagaacataa tctccccggt gtcctgacag
    44641 gcaggacgat tcaggtcaat cgctttttgc atgttgtcga acatcgtatg gtagataatt
    44701 ttccccatcg acgacgtttc ggcatccttt agctgtttta gtttatccac cacgtcatca
    44761 ggcattcggg tagaaatcat ggcggtaaag ttagcgacaa tctctgtcaa cttattcact
    44821 gcctgttgct tattactttc gctcatcatg gcaactccag tttttgtatt tgtctggaaa
    44881 aattcaccgc cacaaactac cactaaaaat gtggttttat attttttgcc accgtgaata
    44941 tgtaattaac agttcgtgaa tcaacgcgaa aataagaatg aatattgcgc cagagcaggg
    45001 aatggcaatg ttttgcgaaa tacactgcgc ttatcacaac tgcggataat taacgatgct
    45061 gaatagctgg cctttagcca aagacttgca ggtactggta gaaatcgtcc acagcgggag
    45121 ttttagcgcc gcagcggcga cgcttgggca gacgcctgca tttgtcacca agcggatcca
    45181 aatccttgaa aatacgctgg caacaacgct gctgaaccgc tccgctcgcg gcgtggcgct
    45241 gactgaaagt ggccaacgtt gttacgaaca tgcgctggaa atcctcaccc aatatcagcg
    45301 actggtcgat gacgtcacgc aaatcaaaac gcgcccggag gggatgattc gtattggctg
    45361 tagttttggt tttgggcgca gccatattgc gccagccatt accgaactta tgcgcaatta
    45421 tcctgagtta caggtgcatt ttgaattgtt cgatcggcaa attgatttag tgcaggataa
    45481 tattgatctg gatattcgta ttaatgacga aattcctgat tattatattg cgcatctgtt
    45541 aacgaaaaat aaaagaatat tatgcgcagc acctgagtat ctgcaaaaat atccgcagcc
    45601 acaatcatta caggaattaa gtcgtcatga ctgtctggtg accaaagagc gcgatatgac
    45661 ccacggaata tgggagttgg ggaacggtca ggagaaaaaa tcggtgaagg ttagcgggca
    45721 tctttcctcc aatagcggcg agatagtgtt gcaatgggcg ctggagggca aagggattat
    45781 gctgcgttcc gagtgggatg tgttgccgtt tctggagagt ggcaaactgg tgcaagtatt
    45841 gccggagtat gcacaaagcg ccaatatctg ggctgtttac cgggagccgc tctatcgcag
    45901 catgaaatta cgtgtctgcg tggaatttct ggcggcatgg tgccagcaac ggctgggcaa
    45961 gcccgatgaa ggctatcagg tcatgtagat ccaccagaaa tcactcggga tccttttcgc
    46021 gctttctttt gaatttcgtc cagatttttg tctcctgacg acgccacaga cgctggatgt
    46081 tgtcatgatg acgcagcagg atcaggcaag agagcatcga aaccgggaag gtgaattgtg
    46141 gcttaaacca ccatacataa aacggagcaa tcagtgcact gacaatcgct cccagcgacg
    46201 agtatccgct caatagcacg gtcagtaacc aggttcccgc cattactccg gtgagatccc
    46261 agccaatggg tgcgatggca ccaaaagcgg tagcaacgcc ttttcctcct ttaaatccga
    46321 agaaaacggg ccagatgtgt ccaagacagg cggcgatggc aattaagcct agccagaagg
    46381 ggctgacacc taattcatac gcgccccaga cgggcaacat tcctttcaga acgtcgaaaa
    46441 tcagtactgc tacggctgct cccttgccac cgatacgtaa cacattggtt gcgcctggat
    46501 tgccggagcc gctggttcgc ggatcgggca gcccacacaa gcggcaaacc agaatggcac
    46561 tggaaatgga gccgcagagg tacgcgatga ggatcattcc aggcgcgatt gcactcataa
    46621 gctgttccgt tttgaaaatt cgtgttcaac gatgaatctg tggataatac gcacatttcg
    46681 ccggaagtgg tatccggtta gccaaaaagc aggcaggacg tgatggatat tgtatttata
    46741 gagcaacttt cggtaatcac cactattggt gtttacgact gggaacagac catcgaacag
    46801 aagttagtgt tcgatatcga aatggcatgg gataaccgta aagcggcgaa aagtgatgat
    46861 gtggcggatt gcctcagtta cgctgacatt gcagaaacgg tggtcagcca cgtcgagggg
    46921 gcgcgttttg cgctggtgga acgcgtggct gaagaggtgg cggagctgct gttagcacgc
    46981 ttcaactcgc cgtgggtgcg tatcaaactc agcaagccag gcgcagtggc gcgggcggcg
    47041 aatgttggcg taatcattga gcgtggcaat aatctgaaag aaaataatta attttacagc
    47101 tgttaaacca aacggttata acctggtctt actgcagtag tccggacaag cggtacattt
    47161 taataattta ggggtttatt gatgagcgat atgcactcgc tgctgatagc ggcaatattg
    47221 ggtgtggtcg aaggattgac agaatttctg ccggtatcca gcacgggcca tatgattatt
    47281 gtcggtcact tgttggggtt cgagggcgac acggcgaaaa cctttgaagt tgtgatccag
    47341 ttaggatcaa ttctggcggt agtagtgatg ttctggcggc gtctgtttgg cctgattggc
    47401 atccactttg gccgcccgtt gcagcacgaa ggagaaagca aaggtcgttt aacgctgatc
    47461 cacattttgc tggggatgat tccggcggtg gtactggggc tgttgttcca cgacacgatt
    47521 aagtcattgt ttaacccgat aaatgtgatg tatgcactgg tcgttggcgg tttgttgctg
    47581 attgccgccg aatgcctgaa gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc
    47641 tatcgtcagg cgtttatgat tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc
    47701 cgttccgggg ccaccatttc aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc
    47761 gagttctcgt tcctgctggc ggtaccgatg atgatgggcg caacggcgct cgatctctac
    47821 aaaagctggg gcttcctgac aaccggcgat atcccgatgt ttgccgttgg gtttatcacc
    47881 gcttttgtgg tggcgctgat agcgattaaa accttcctgc aattgattaa gcgcatttcg
    47941 tttatcccgt tcgccattta tcgctttatt gtggcggctg cggtgtacgt cgtgttcttt
    48001 tgattgagaa aaggttcggg agcaggcctg gttttctccc tcgctctgta gggagagggc
    48061 cggggtgggg gtgattgttc gcaccggtgc tggtctgttc ccctcaccct aaccctctcc
    48121 ccgaaggggc gaggggatgg ttcgtgttaa cgtttagttt tatactcagt caggctttgg
    48181 gcaacgttgt tccttccaac tggctaccgc cgcaatccgc cgtcgggtca gctcctcgcg
    48241 aatctccaca cctttaaatc ccgcttcaac gaccgctttt gtcggcactg actgcgccac
    48301 ttcccaggct tcgcgcaacc aacggccttg cgggtagtcc gctgattcaa aaccggttct
    48361 gccgcgtacg tcagcctcgc tggtcagcgc cagttgctcg acacgctgcg gtttacgcca
    48421 ggcatcgatg gaatcaaata atttgacgat ggtttttggg ttcagcattg ggaaggtgtg
    48481 gatgagatcg tgaaactcag ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg
    48541 cagacgctgg catagttgtt ccactaactt aacacccgcc gggccatgcc catgatgacg
    48601 cggccaaagt tctggcggcg tcagtccttt accgagatcg tggcataaag ttgcgaaacg
    48661 gacatcaacc tgcggactca gcatcgccgc cattgagagc gtcattaagg tatgaatacc
    48721 cgtgtcgatt tccggatgcc acctggcagg ggccggaacg ccaaacaggg cgtcaatttc
    48781 cgggaataaa acgcgaagtg caccgcaatc gcgcagtacc tggaagaaca cctggggatt
    48841 gcgggtggtg agggcgcttt ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag
    48901 ttcacccgca tgggtcatct cgcgcatcag cgtcagagtt tcatcggcaa tacgaaaacc
    48961 gaggtgggca taacgcgcag caaaacgcgc cacgcgcaga acgcgtaacg gatcttcgcc
    49021 aaaagcgggg gaaacatggc gcaacagacg attttgcaga tcgcccagac cgttgtacgg
    49081 gtcgataatc tcgccgttat cgtcctgagc cagcgcatta atggtcagat cgcgacgctt
    49141 aagatcatct tccagcgtga catccggtgc ggcatagcaa gtaaaaccgg tgtaaccgga
    49201 accggatttc cgttcggtac gtgccagcgc atactcttca tgcgtttgcg gatgcagaaa
    49261 cacaggaaaa tcgcggccta cctgctggta gcccgcgtcg agcatctcct gtggcgtact
    49321 gccgaccacc acccaatctc tgtctttgac cggtagccct aacaatgcat cccgaacagc
    49381 accaccgacc agataaatct tcacgccatc atcccttcgt caaatacaca attcctaata
    49441 ataagacagt gcaacgaaga aggcgattta gttcatccag cgatctttgc gtttgcgcgt
    49501 tgggatcagg tgtggcagta ccagaccgag cagcaagcca agccccagca cgccaccgcc
    49561 atacataaac cattgcatga tgatggtgcg ctgtttgtca tccagctgta cgctggcggc
    49621 atcgaccttt ttctgcgcga caatcagctc gtttttcaat ttctgatttt cttcttttaa
    49681 tccgttgatc acgctgtcgc tctgcgccac tttttgctgc atttctgcgg tgcgctgatt
    49741 ccaggtgtta tcgatattgg tgagtttatc ggtcagggtt ttgacctgat tttccagatc
    49801 tggcacacgg gagcgcaggc ttggctcagt gctaagttgt ttcaacggga tccaggcggt
    49861 acggccagag ctgtctttca cctgggcata attggtgttg gcgtcagttt gtaataaggt
    49921 cacttcctcg ccggcgttaa ccgtgcccac gaggcgataa tgatctcccg gaccgctacg
    49981 gacccaggta ttcagttcat cagaaacata gcgcgtttct tcagcgtgtg agacggcagt
    50041 ggcgctaagt gcgagtaaag ttaatccgat caggcgtaat tttggcatca ggctgtcgtt
    50101 attgtcatgg aaagtggaac gatagtagtg gcatcagtgt cgctacgcaa agcattcgac
    50161 atcaatcgga atcctctgtg tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa
    50221 ttgcccgttg catggcaatt tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta
    50281 agttcgccgt tcatcgtcac ttatgtgaca taaccgataa gtaaaggcca tggctcagga
    50341 aatcgaatta aagtttattg ttaatcacag tgccgttgag gcgttgcgtg accatctcaa
    50401 tacgctgggc ggcgagcacc atgaccccgt gcagttgctg aatatttact acgaaacgcc
    50461 ggataactgg ctgcgtgggc acgatatggg cttacgtatt cgtggcgaaa acggtcgcta
    50521 tgagatgacc atgaaagttg caggaagagt gacaggcggc ttacaccagc gcccggaata
    50581 taacgtggcg ttgagcgaac cgacgctcga cctggcgcag ttaccgacgg aagtctggcc
    50641 gaacggcgaa ttgcccgccg atctcgcttc ccgcgtacaa ccgctgttca gcaccgattt
    50701 ttatcgcgaa aaatggctgg tggcggtcga tgacagccga attgaaatcg ctctcgatca
    50761 gggggaggtg aaagcggggg aatttgctga acctatctgt gagctggaac tggaactgct
    50821 tagcggcgac acgcgcgcgg tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt
    50881 acgccagggc agcctgagca aagcggcgcg tggctatcat ctggcgcagg gcaatccggc
    50941 gcgtgaaatc aaaccgacca ccattttgca tgttgcggca aaagccgatg tggaacaggg
    51001 gctggaagcg gcgctcgagc tggcgttagc gcaatggcag tatcatgaag aactgtgggt
    51061 acgcggcaac gatgcggcga aagaacaggt gctggcagcc attggtctgg tccgtcatac
    51121 actgatgctg ttcggtggta ttgtgccgcg caaagcgagc actcacttac gtgatttgct
    51181 gactcaatgc gaagcgacca tcgcttctgc ggtttctgcc gtgacggcgg tctactctac
    51241 caaaacggca atggcgaagc tggcgttgac cgaatggctg gtaagcaaag cctggcagcc
    51301 gtttttagat gccaaagcgc agagtaaaat gagcgattcc ttcaaacgct ttgccgatat
    51361 ccatctttcc cgccatgccg ctgaactgaa aagcgttttc tgccagccgt tgggcgatcg
    51421 ctaccgtgac cagttgccac gtctgacgcg tgatattgac tcaatactgt tgctggcggg
    51481 ttactatgat cctgtcgtcg cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc
    51541 tattgcaacc gggcaacgca ttgaaattga acatttccgt aatgaggcaa acaatcagga
    51601 accgttctgg ttgcacagcg gaaaacgtta atcaggcaag gataaaatcg cttatgaagc
    51661 cgctctcttc accgttacag cagtactggc agaccgttgt tgagcggctg ccagagcctt
    51721 tagccgagaa atcacttagc gcacaggcga agtcagtact tactttcagt gattttgtgc
    51781 aggacagcgt gattgcgcat ccagagtggc tgacggaact ggaaagccaa tcgccgcagg
    51841 ccgacgaatg gcagcattac gcggcatggt tgcaggaggc gctcagcaat gtgagtgacg
    51901 aagccgggtt aatgcgcgag ctgcggctat tccggcggcg cattatggtg cgcatcgcct
    51961 gggcgcaaac gctggctttg gttactgaag agagcatatt gcagcagctc agccatctgg
    52021 cggagacgct gattgtcgcc gcgcgtgact ggctgtatga cgcttgctgc cgcgagtggg
    52081 gaacgccgtg caatgcgcag ggcgaagcgc aaccgctgct gattttaggc atgggtaagc
    52141 tgggcggtgg ggaactgaat ttctcctcag atatcgatct gatttttgcc tggccggaac
    52201 atggttgtac gcagggcgga cgccgggaac tggataacgc gcagtttttt acccgcatgg
    52261 ggcagcggct gattaaagtg ttggatcaac caacgcagga tggcttcgtc tatcgcgtgg
    52321 atatgcggct gcgtccgttt ggcgaaagtg gcccgctggt gctgagcttt gccgcgttgg
    52381 aggattatta ccaggagcag gggcgcgact gggagcgtta cgcgatggtc aaggcgcgga
    52441 ttatgggcga tagcgaaggc gtctatgcta acgagttgcg tgcgatgctg cgcccgtttg
    52501 ttttccgtcg ttacatcgat ttcagcgtga ttcagtcgct gcgcaacatg aaagggatga
    52561 ttgcccgtga agtgcgtcga cgtggtttga ccgacaatat caaactcggc gcaggcggca
    52621 ttcgcgaaat tgaatttatc gttcaggtgt tccagctcat tcgcggtggg cgcgaaccgt
    52681 cgctgcagtc gcgctcttta ctgccaacgc tcagcgtcat tgccgcgctg catctgcttt
    52741 ctgaaaacga tgctgaacaa ttgcgagtgg cgtatctgtt cctgcggcgt ctggaaaacc
    52801 tgctgcaaag cattaacgac gaacaaaccc agacgcttcc ttctgatgag cttaaccgtg
    52861 cgcggctggc gtgggcgatg gactttgctg actggccgca actgaccggg gcgctgaccg
    52921 cacatatgac caatgtgcgc cgggtgttta atgaattgat tggcgacgac gaaagtgaaa
    52981 ctcaggaaga gtcgctgtcg gaacagtggc gtgagctgtg gcaggatgcg ttgcaggaag
    53041 atgacactac gccagtgctg gcgcatctta gcgaggatga tcgcaaacag gtgctaacgc
    53101 tgattgccga tttccgcaaa gagctggata agcgcaccat cgggccgcga ggacgtcagg
    53161 tgctcgacca tctgatgccg catctgctaa gtgatgtctg tgcgcgtgaa gacgctgccg
    53221 ttacgctgtc gcgcattacc gccttgctgg tggggattgt tacccgcacc acctatttag
    53281 aattgctcag tgaattcccc gcggcgctta aacatttgat ttctctgtgt gccgcgtcgc
    53341 cgatgattgc cagccaactg gcgcgttatc cattattgct ggatgaattg ctcgatccaa
    53401 acacccttta ccagccgacg gcgaccgatg cctaccgcga tgagttgcgc cagtatttgc
    53461 tgcgcgtgcc ggaagatgac gaagagcaac agcttgaggc gctgcgtcag ttcaaacagg
    53521 cgcagctgtt acgcatcgcc gcagcggata tcgctggtac gctaccggtg atgaaagtga
    53581 gcgatcactt aacctggctg gcagaggcta tgatagatgc cgtcgttcag caggcgtggg
    53641 ttcaaatggt ggcccgctac ggtaagccaa atcacctcaa cgatcgcgaa ggccgtggtt
    53701 ttgcggtggt cggctacggc aagctgggcg gctgggagtt aggctacagt tccgatcttg
    53761 acctgatctt cctccatgat tgcccaatgg atgcgatgac tgacggcgag cgggaaatcg
    53821 acgggcgaca gttttatctg cgtctggcgc aacgcattat gcatctgttc agtacgcgta
    53881 cctcttccgg cattttgtat gaagtggatg cacggctacg cccgtccggg gccgcgggaa
    53941 tgctggtgac atccgcagaa gcatttgccg attatcagaa aaacgaggcc tggacgtggg
    54001 aacatcaggc gctggtgcgt gcgcgggtag tgtacggcga tccgcagctc accgcgcact
    54061 ttgacgcggt gcgtcgcgag attatgacgc tgccgcgtga aggtaaaact ctgcaaacgg
    54121 aagtgcggga aatgcgcgag aaaatgcgcg ctcatctcgg caataaacat cgcgatcgct
    54181 ttgatatcaa agctgatgaa ggcggaatta ccgatatcga atttattacc caatatctgg
    54241 tgttgcgcta cgctcatgaa aaaccgaagt taacgcgctg gtcagacaac gtgcgcattc
    54301 tggaactact ggcgcaaaac gacattatgg aagagcagga agcgatggcg ctgacccgtg
    54361 cttatactac gctgcgcgat gaacttcatc atctggcatt acaggaattg ccgggccatg
    54421 tgccggagga ttgcttcacc gcagagcgtg aactggtgcg ggcaagctgg cagaagtggc
    54481 tggtggaaga atgaagtatg gtattatcgc gcgcaaattt tgaatctctc aggagacagg
    54541 aatgaaagta acgctgccag agtttgaacg tgcaggagtg atggtggttg gtgatgtgat
    54601 gctggatcgt tactggtacg gtcccaccag ccgtatctcg ccggaagcgc cggtgcccgt
    54661 ggttaaagtc aataccatcg aagaacgtcc gggcggcgcg gctaacgtgg caatgaacat
    54721 cgcttctctc ggtgccaatg ctcgcctggt cgggctgacg ggcattgacg atgcggcgcg
    54781 tgcgctgagt aaatctctgg ccgacgtcaa cgtcaaatgc gacttcgttt ctgtaccgac
    54841 gcatccgact atcaccaaat tacgggtact ttcccgcaac caacagctga tccgcctgga
    54901 ttttgaagaa ggtttcgaag gtgttgatcc gcagccgctg cacgagcgga ttaatcaggc
    54961 gctgagttcg attggcgcgc tggtgctttc tgactacgcc aaaggtgcgc tggcaagcgt
    55021 acagcagatg atccaactgg cgcgtaaagc gggcgttccg gtgctgattg atccaaaagg
    55081 taccgatttt gagcgctacc gcggcgctac gctgttaacg ccgaatctct cggaatttga
    55141 agctgttgtc ggtaaatgta agaccgaaga agagattgtt gagcgcggca tgaaactgat
    55201 tgccgattac gaactctcgg ctctgttagt gacccgttcc gaacagggta tgtcgctgct
    55261 gcaaccgggt aaagcgccgc tgcatatgcc aacccaggcg caggaagtgt atgacgttac
    55321 cggtgcgggc gacacggtga ttggcgtcct ggcggcaacg ctggcagcgg gtaattcgct
    55381 ggaagaagcc tgcttctttg ccaatgcggc ggctggcgtg gtggtcggca aactgggaac
    55441 ctccacggtt tcgccgatcg agctggaaaa tgcagtgcgt gggcgcgcag ataccggctt
    55501 tggtgtgatg accgaagagg aactgaagct tgctgtagcg gcagcgcgta aacgcggcga
    55561 aaaggtggta atgaccaacg gtgtctttga catcctgcac gccgggcacg tctcctatct
    55621 ggcaaatgct cgcaaactgg gtgaccgtct gattgtcgct gtgaacagcg atgcctccac
    55681 caaacggctg aaaggggatt cccgcccggt aaacccactc gagcagcgta tgattgtgct
    55741 gggcgcactg gtagcggtcg actgggtagt gtcgtttgaa gaagacacgc cgcagcgtct
    55801 gatcgccggg atcctgccag atctgctggt gaaaggcggc gactataaac cagaagaaat
    55861 tgccgggagt aaagaagtct gggccaacgg cggcgaagtg ctggtgctca acttcgaaga
    55921 cggttgctcg accactaaca tcatcaagaa gatccaacag gataaaaaag gctaaccgga
    55981 aagcggttca cagatctcgc ttcgtagcag gcaggcctat tgacgctgtt gttttttcaa
    56041 cctaaagtaa aggaacaagg gtaagggagg atttctcccc cctctgatca gttgttagta
    56101 agtcgggaaa cttaacagta acaacacaac cagtatgatg acgaacttca tcataaccct
    56161 ttccttctat aaggcccctt cctcgggagg ggctttcccg tttcagcccc ttgctgactc
    56221 cccgattgtt gatgagcgcc ggaccccgcg caacgctttc cctgaagcag ttttgccttc
    56281 cccgagcggt cacgcaaaac gctgcaacgt cctgagcatt tgcacctttt agcggaacgc
    56341 agatataaaa aatccccggc tttttgggcc agggatatgt gttaatgaaa ccttcttgac
    56401 gctgattttt tttcaaccta aagtaaagga acaagggtga gggaggattt ctcccccctc
    56461 tgattggctg ttaataagct gcgaaactta cgagtaacaa cacaatcagt atgatgacga
    56521 gcttcatcat aaccctttcc ttctgtaagg ccccttcctc gggaggggct ttcccgtttc
    56581 agccctttgc tgactccccg attgttgatg agcgccggac ctcgcgcaac gctttcccag
    56641 aggcagtttt gccttccccg agcggtcacg caaaacgttg caacgacctg atcatttgca
    56701 gtttttagcg gaacgcagat ataaaaaatc cccggctttt tgtgccaggg acgttcatag
    56761 ctgaaaattt attctacgtt ttcggcgacg ggagttgttg aggttaaggg tgaagtcaat
    56821 gccgccagtg agccgcctga aacgccaatt tcattgagca aggagtcaat cagcggtgcc
    56881 tgtgtgcggt aagagagagc ggctgataat gcttgttccg ccaggtttcc gccccccaca
    56941 ttacccgtgt ttgcatcacc cgcagcgctg ccacgattca gaccatcgac ctgaataatc
    57001 ttgataccgt caatagattt catcggctca acggattttt ctattaccgc aggtagcgcc
    57061 tgcaacaagg ccagtttgaa tttaaggctg gtttgttcat cagaaagtac gttgatagca
    57121 tcgttcagcg cacgttgtgc ttctgcttcc gccagaccct ttttacgtgt agcttcggct
    57181 aactcaacga tagccgccgc ctgcatctct gcggcttctt tttctgcttt cgcccgcacg
    57241 gtcagttcaa ccgctttggt ttctgcatcc tgcgcggcag cgattagggc aacttgttta
    57301 gcacgatcgg cttctgcggt ctggcgagtg gtttctacgt tttgttgcgc acttactgct
    57361 tctgcgagtg caaggttagc acgcgcttct gcctgggact gttgttccga tttggcggca
    57421 atagcgatcg atttcgtctg gttggcgatt tcggtgacct gctgttgttc gatctcttta
    57481 atacgaactt cacgttcagc ctcaaccttt cttgagcgga cggcctgctc gcgctcgatc
    57541 tccgtttcct gaatttgtcg ttcagccaga attcgcgtct gctctgcttc acgacgacgt
    57601 tcagcttcaa aagccgcaat acgtgcattc tgttcggcag tacgggtttt aacctgctgc
    57661 tcctgctcaa gcgtcataaa cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca
    57721 tcacggtttt tctcacgcac cgcaacttct acatcctgtt caacttcgtt acgttcgcga
    57781 cgacggcgct ccgtctcctg agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc
    57841 ggattgaaat gttctttcga ggtctggtta aagttggtaa gtgaaacgct ttccagctca
    57901 agaccgtttt tcgacaggtc ttctgcgaca gtattttgta ctccctgcac gaagttctcg
    57961 cgggtatcct gtaactcatg catggtcatt tgtgcagctg ttgcacggag ggcatcgaca
    58021 aatttatctt caaccaacat acgtaagtct tctggtgata gcgtgcgttg ccccaacgtc
    58081 tgcgccgcag tggcgatgcc ttctacagac ggttttaccc gtacaaagaa agcgactact
    58141 acatcgacac gcatacgatc tttcgtaatc aggctatcaa tggttgagcg gctgacttcc
    58201 agcttcagag tattcatatt gatggggatt atttcatgaa agatcggcat cacgattgcg
    58261 ccaccgctca tcaccacttt ttgcccacct aaaccagtac gaacaaaagc ttgctctgcc
    58321 gaagcgcgac gatagagcct ggcgaaaata attccaataa taaacagaat gcatacggca
    58381 ataatcgcgg taaacatcca ggagggcaca gaattaacaa tatcatccat tattaaattt
    58441 ccttttgatt aataaattac aatatttgtg gccagggatt gttttccgcc aaatatcgcg
    58501 ttgcagaaag tcggcaaata attaacactt tgtctccttt agtgaaaaat tttccttctt
    58561 caggctccag tagtaaataa tgtatttggc caaattgatc ggtgagtttt ccttcgcagg
    58621 gattacctga agtggcttga tgaccggtaa ttaatgccat gctaccaata tattcttctt
    58681 ctgtaatagc tgaactatgg tcgcgtggaa tccagggcgc aactatttta ccggtataat
    58741 gcaccgcaat aatcgtaaac agtaaactga caggaaccac gaacaggttc gacagtggtg
    58801 attgccagac catgatgcag gcatgctgca acaggatgcc aataagacca aaaaaaccag
    58861 ccagcaagca gagaacgacg agagctggca atcttccgat attgagataa tgaagtgcct
    58921 ggctaatatg acctgtggtt atggaatcat aatggtcaag atgtgcatca agagcaccgg
    58981 agagcatgtg accgcagata agcgctaaaa tttctaaaag gccgatcaac agtacaaagg
    59041 aaatagcgaa aagataaggg gtattatagt cggcgaataa aatcatatgt cactccaatg
    59101 tccatttagt gacgcaatat ttactttggg atagtttcct tgcttacatt tatctttgtt
    59161 aataataaca catgttagag aagttctgga agagaaagtt ttttaatatg gttttttatt
    59221 tttcactaaa aagtgtgatc ggggacaata tatttacgca cgttatgttt aaaggcacta
    59281 cactgattgg ggaatactga aatcagaaaa agcatatggc cttaaaaggt atgcgataac
    59341 cggaggatgt gcttatggat catagtctta attctttaaa taatttcgat ttcctggcgc
    59401 gtagttttgc cagaatgcac gcagaaggtc gcccggtcga tattcttgcc gttactggta
    59461 acatggatga agaacataga acctggtttt gcgcacgtta tgcctggtat tgtcaacaga
    59521 tgatgcaggc aagagagctg gagttagagc actgatataa cgggcctgat ggcccgtttt
    59581 agtgtttgta ttagatgcta gttataggtg attttgagga tgacgtcttt actaaacggt
    59641 ccctcaacaa cagtctctgc cggattcgca ctaagttctg ccctgaatgg tgcggtgacc
    59701 gaaggtgcac tagacgatga aaaagaagca aattgtgaga agcggttaaa ttccgttgct
    59761 gttccatctt tatgaaatat cttcaatagc agtccattac ccatatccag atggctataa
    59821 tcactcaaaa tatccgtagt gaaaaagctc cctaaaatat tgaattgctc agtacaggcc
    59881 gcaccaggat cttttgtcat gactacacta aacgtctcac taacgctggt atttttaatt
    59941 gttcgggaat ttattttacc aaagtcaaca acgccatcag ctggttccag tgcgacatta
    60001 acaaagcagt caaggacatg aatattattt aatccacgaa tcctgaaggt caaatttttg
    60061 tctacttcta tagctcctgc gccactcata cctttaaact ctagagcgat atattcatca
    60121 ggtaacacca ggtttttatt aactctattg gcattttttg ctaatttaat atagagttgt
    60181 acgcgagctg gaaaggttac tgataattga ggctcaggac ctccactaca gacagcgggg
    60241 tttttatagt gattattaaa ggtacccgct ccttttatat caatgcattc tggtgctgcg
    60301 atagcattag gggcagtcga atcataatca actccattgt atgttacgcc aaaaatatag
    60361 gagttctgat tataaaagtc ctgaccgcct ggtaaaaaca tcattatatt agtccaggct
    60421 gatgggtatt cttctccagg tgatggggca ggtaatgcat tattacagaa gacattcata
    60481 aaatactcac tgctttgcca gatcttatca ccttttttac cattactggg gatagaaatt
    60541 cttggtacgt aaccaatgac tacggtattt cccccttcat gttcagcata gcagtccatt
    60601 g
//
LOCUS       DAZGCS010000015        60363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGCS010000015 DAZGCS010000000
VERSION     DAZGCS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.015"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..151)
                     /locus_tag="ACHK01_001000"
     CDS             complement(<1..151)
                     /locus_tag="ACHK01_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO8095990.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYF"
     gene            complement(204..1460)
                     /gene="xapB"
                     /locus_tag="ACHK01_001001"
     CDS             complement(204..1460)
                     /gene="xapB"
                     /locus_tag="ACHK01_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO8095991.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(1520..2353)
                     /gene="xapA"
                     /locus_tag="ACHK01_001002"
     CDS             complement(1520..2353)
                     /gene="xapA"
                     /locus_tag="ACHK01_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO8095992.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2602..3366
                     /gene="yfeN"
                     /locus_tag="ACHK01_001003"
     CDS             2602..3366
                     /gene="yfeN"
                     /locus_tag="ACHK01_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO8095993.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3405..4331)
                     /gene="yfeR"
                     /locus_tag="ACHK01_001004"
     CDS             complement(3405..4331)
                     /gene="yfeR"
                     /locus_tag="ACHK01_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8095994.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4421..5419
                     /gene="yfeH"
                     /locus_tag="ACHK01_001005"
     CDS             4421..5419
                     /gene="yfeH"
                     /locus_tag="ACHK01_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO8095995.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(5416..5634)
                     /gene="ypeB"
                     /locus_tag="ACHK01_001006"
     CDS             complement(5416..5634)
                     /gene="ypeB"
                     /locus_tag="ACHK01_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO8095996.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5636..7651)
                     /gene="ligA"
                     /locus_tag="ACHK01_001007"
     CDS             complement(5636..7651)
                     /gene="ligA"
                     /locus_tag="ACHK01_001007"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO8095997.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7722..8708)
                     /gene="zipA"
                     /locus_tag="ACHK01_001008"
     CDS             complement(7722..8708)
                     /gene="zipA"
                     /locus_tag="ACHK01_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO8095998.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8938..9699
                     /gene="cysZ"
                     /locus_tag="ACHK01_001009"
     CDS             8938..9699
                     /gene="cysZ"
                     /locus_tag="ACHK01_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO8095999.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9884..10855
                     /gene="cysK"
                     /locus_tag="ACHK01_001010"
     CDS             9884..10855
                     /gene="cysK"
                     /locus_tag="ACHK01_001010"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO8096000.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11239..11496
                     /gene="ptsH"
                     /locus_tag="ACHK01_001011"
     CDS             11239..11496
                     /gene="ptsH"
                     /locus_tag="ACHK01_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO8096001.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11541..13268
                     /gene="ptsI"
                     /locus_tag="ACHK01_001012"
     CDS             11541..13268
                     /gene="ptsI"
                     /locus_tag="ACHK01_001012"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO8096002.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13309..13818
                     /gene="crr"
                     /locus_tag="ACHK01_001013"
     CDS             13309..13818
                     /gene="crr"
                     /locus_tag="ACHK01_001013"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO8096003.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13860..14711)
                     /gene="pdxK"
                     /locus_tag="ACHK01_001014"
                     /gene_synonym="thiJ"
     CDS             complement(13860..14711)
                     /gene="pdxK"
                     /locus_tag="ACHK01_001014"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO8096004.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14816..15184
                     /gene="yfeK"
                     /locus_tag="ACHK01_001015"
     CDS             14816..15184
                     /gene="yfeK"
                     /locus_tag="ACHK01_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO8096005.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15187..16098)
                     /gene="cysM"
                     /locus_tag="ACHK01_001016"
     CDS             complement(15187..16098)
                     /gene="cysM"
                     /locus_tag="ACHK01_001016"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO8096006.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16232..17329)
                     /gene="cysA"
                     /locus_tag="ACHK01_001017"
     CDS             complement(16232..17329)
                     /gene="cysA"
                     /locus_tag="ACHK01_001017"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO8096007.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17319..18194)
                     /gene="cysW"
                     /locus_tag="ACHK01_001018"
     CDS             complement(17319..18194)
                     /gene="cysW"
                     /locus_tag="ACHK01_001018"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO8096008.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18194..19027)
                     /gene="cysT"
                     /locus_tag="ACHK01_001019"
     CDS             complement(18194..19027)
                     /gene="cysT"
                     /locus_tag="ACHK01_001019"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO8096009.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(19027..20043)
                     /gene="cysP"
                     /locus_tag="ACHK01_001020"
     CDS             complement(19027..20043)
                     /gene="cysP"
                     /locus_tag="ACHK01_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO8096010.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(20214..21005)
                     /gene="ucpA"
                     /locus_tag="ACHK01_001021"
     CDS             complement(20214..21005)
                     /gene="ucpA"
                     /locus_tag="ACHK01_001021"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO8096011.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21134..21991)
                     /gene="murR"
                     /locus_tag="ACHK01_001022"
     CDS             complement(21134..21991)
                     /gene="murR"
                     /locus_tag="ACHK01_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO8096012.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            22155..23051
                     /gene="murQ"
                     /locus_tag="ACHK01_001023"
     CDS             22155..23051
                     /gene="murQ"
                     /locus_tag="ACHK01_001023"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO8096013.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23055..24479
                     /gene="murP"
                     /locus_tag="ACHK01_001024"
     CDS             23055..24479
                     /gene="murP"
                     /locus_tag="ACHK01_001024"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO8096014.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24484..25785
                     /gene="pbp4b"
                     /locus_tag="ACHK01_001025"
     CDS             24484..25785
                     /gene="pbp4b"
                     /locus_tag="ACHK01_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO8096015.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(25843..26742)
                     /gene="yfeX"
                     /locus_tag="ACHK01_001026"
     CDS             complement(25843..26742)
                     /gene="yfeX"
                     /locus_tag="ACHK01_001026"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO8096016.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26838..27413)
                     /gene="yfeY"
                     /locus_tag="ACHK01_001027"
     CDS             complement(26838..27413)
                     /gene="yfeY"
                     /locus_tag="ACHK01_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO8096017.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27474..27923)
                     /gene="yfeZ"
                     /locus_tag="ACHK01_001028"
     CDS             complement(27474..27923)
                     /gene="yfeZ"
                     /locus_tag="ACHK01_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO8096018.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(27910..28335)
                     /gene="ypeA"
                     /locus_tag="ACHK01_001029"
     CDS             complement(27910..28335)
                     /gene="ypeA"
                     /locus_tag="ACHK01_001029"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO8096019.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28549..29418
                     /gene="amiA"
                     /locus_tag="ACHK01_001030"
     CDS             28549..29418
                     /gene="amiA"
                     /locus_tag="ACHK01_001030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO8096020.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29422..30321
                     /gene="hemF"
                     /locus_tag="ACHK01_001031"
     CDS             29422..30321
                     /gene="hemF"
                     /locus_tag="ACHK01_001031"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO8096021.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30327..31379)
                     /gene="eutR"
                     /locus_tag="ACHK01_001032"
     CDS             complement(30327..31379)
                     /gene="eutR"
                     /locus_tag="ACHK01_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO8096022.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31425..31925)
                     /gene="eutK"
                     /locus_tag="ACHK01_001033"
     CDS             complement(31425..31925)
                     /gene="eutK"
                     /locus_tag="ACHK01_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO8096023.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(31938..32597)
                     /gene="eutL"
                     /locus_tag="ACHK01_001034"
     CDS             complement(31938..32597)
                     /gene="eutL"
                     /locus_tag="ACHK01_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO8096024.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32607..33494)
                     /gene="eutC"
                     /locus_tag="ACHK01_001035"
     CDS             complement(32607..33494)
                     /gene="eutC"
                     /locus_tag="ACHK01_001035"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO8096025.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33515..34876)
                     /gene="eutB"
                     /locus_tag="ACHK01_001036"
     CDS             complement(33515..34876)
                     /gene="eutB"
                     /locus_tag="ACHK01_001036"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO8096026.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(34888..36291)
                     /gene="eutA"
                     /locus_tag="ACHK01_001037"
     CDS             complement(34888..36291)
                     /gene="eutA"
                     /locus_tag="ACHK01_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO8096027.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36288..37514)
                     /gene="eutH"
                     /locus_tag="ACHK01_001038"
     CDS             complement(36288..37514)
                     /gene="eutH"
                     /locus_tag="ACHK01_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO8096028.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(37631..38818)
                     /gene="eutG"
                     /locus_tag="ACHK01_001039"
     CDS             complement(37631..38818)
                     /gene="eutG"
                     /locus_tag="ACHK01_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO8096029.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(38808..39644)
                     /gene="eutJ"
                     /locus_tag="ACHK01_001040"
     CDS             complement(38808..39644)
                     /gene="eutJ"
                     /locus_tag="ACHK01_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO8096030.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(39655..41058)
                     /gene="eutE"
                     /locus_tag="ACHK01_001041"
     CDS             complement(39655..41058)
                     /gene="eutE"
                     /locus_tag="ACHK01_001041"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO8096031.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41070..41357)
                     /gene="eutN"
                     /locus_tag="ACHK01_001042"
     CDS             complement(41070..41357)
                     /gene="eutN"
                     /locus_tag="ACHK01_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO8096032.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41464..41757)
                     /gene="eutM"
                     /locus_tag="ACHK01_001043"
     CDS             complement(41464..41757)
                     /gene="eutM"
                     /locus_tag="ACHK01_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO8096033.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(41796..42812)
                     /gene="pta"
                     /locus_tag="ACHK01_001044"
     CDS             complement(41796..42812)
                     /gene="pta"
                     /locus_tag="ACHK01_001044"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8096034.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     PLGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(42809..43612)
                     /gene="eutT"
                     /locus_tag="ACHK01_001045"
     CDS             complement(42809..43612)
                     /gene="eutT"
                     /locus_tag="ACHK01_001045"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO8096035.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(43609..44310)
                     /gene="eutQ"
                     /locus_tag="ACHK01_001046"
     CDS             complement(43609..44310)
                     /gene="eutQ"
                     /locus_tag="ACHK01_001046"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO8096036.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44285..44764)
                     /gene="eutP"
                     /locus_tag="ACHK01_001047"
     CDS             complement(44285..44764)
                     /gene="eutP"
                     /locus_tag="ACHK01_001047"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO8096037.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLMDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(44777..45112)
                     /gene="eutS"
                     /locus_tag="ACHK01_001048"
     CDS             complement(44777..45112)
                     /gene="eutS"
                     /locus_tag="ACHK01_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO8096038.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45405..47684)
                     /gene="maeB"
                     /locus_tag="ACHK01_001049"
     CDS             complement(45405..47684)
                     /gene="maeB"
                     /locus_tag="ACHK01_001049"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO8096039.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47973..48923
                     /gene="tal"
                     /locus_tag="ACHK01_001050"
     CDS             47973..48923
                     /gene="tal"
                     /locus_tag="ACHK01_001050"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8096040.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            48943..50946
                     /gene="tkt"
                     /locus_tag="ACHK01_001051"
     CDS             48943..50946
                     /gene="tkt"
                     /locus_tag="ACHK01_001051"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8096041.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(51042..52085)
                     /gene="ypfG"
                     /locus_tag="ACHK01_001052"
     CDS             complement(51042..52085)
                     /gene="ypfG"
                     /locus_tag="ACHK01_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO8096042.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(52211..52786)
                     /gene="nudK"
                     /locus_tag="ACHK01_001053"
     CDS             complement(52211..52786)
                     /gene="nudK"
                     /locus_tag="ACHK01_001053"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO8096043.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(52854..54833)
                     /gene="aegA"
                     /locus_tag="ACHK01_001054"
     CDS             complement(52854..54833)
                     /gene="aegA"
                     /locus_tag="ACHK01_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO8096044.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            55039..56739
                     /gene="narQ"
                     /locus_tag="ACHK01_001055"
     CDS             55039..56739
                     /gene="narQ"
                     /locus_tag="ACHK01_001055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO8096045.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            56903..60016
                     /gene="acrD"
                     /locus_tag="ACHK01_001056"
     CDS             56903..60016
                     /gene="acrD"
                     /locus_tag="ACHK01_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO8096046.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60115..60174)
                     /gene="ypfM"
                     /locus_tag="ACHK01_001057"
     CDS             complement(60115..60174)
                     /gene="ypfM"
                     /locus_tag="ACHK01_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO8096047.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14156 a        16627 c        15401 g        14179 t
ORIGIN      
        1 taaagtattt caccaccaga tatccgacat acataaacac aatggataat atacctggta
       61 atacatttgg tacaggaata ggcaagcgca cgattgtaaa caaagcacca atgccaaaac
      121 caacgccgga agaaaataaa atatctttca ctaattaacc tttatcataa aagcagctct
      181 gaagagcaga gctgcgaatc cttttaatga gccaccgctc gatgctttat cttttcaggg
      241 tcatgattat atttaaaccc aaagaaaaat atcactgcga gaaaaagagc atatcctgca
      301 aacaccagcc agatagtttg ccagtctttt acgccatcca ccgaaaagta atctactgcc
      361 atgccactca gaatcgagcc aacccatgcg ccgacaccat ttaccatggt cataaagagc
      421 ccctgcgcgc tggcacgaat gctggaatca acttcctgtt cgacaaatac cgaaccagaa
      481 atattgaaga aatcgaatgc acagccataa acaatcatcg acagcagcag caaaataaat
      541 ccggttgttg acggatcgcc ataggcgaag aagccaaagc gcagcgtcca ggccaccata
      601 ctcatcagca tgacggtttt aatgccaaat cgctttaaaa agaatgggat agtcagtata
      661 aagcccactt ctgccatctg tgaaactgac agtaaaatgg agggatattt caccacaaaa
      721 ctgtcagcaa actccgggtt acgggcgaaa tcatgtagga acggattacc aaaaacgttg
      781 gtaatttgca gtaccgcacc cagcatcatg gcaaagagga aaaagatggc catgcgtgga
      841 tttttaaaca gcacgaaggc atccagaccc agcttgctgg caagcgatgt ggtcgctttt
      901 ttctccgcaa ccggaatctt cggcaaagtc agcgcataag ccgacagcag caatgacgca
      961 ccggacgcga tatacagctg cagactactc aattccagat gcagcaggct tactgcccac
     1021 atcgcgacaa tgaaccccac cgtaccaaaa acgcgaatgg gcgggaaagc ggtcaccggg
     1081 tcaagccctg cctgggcaag acaggaataa gagacgctgt tcgataacgc aatagtcggc
     1141 ataaacgcca tcgcattgac taacatcacc caaaacatca tatccggatc agttacggat
     1201 gccgcataaa aaagtacgcc cgcacacacc aggtgacaca gcatgtatgc acgttctgcg
     1261 cgcagccatt tgtctgcgat gatccccatt ataccaggca taataatcgc ggcgatccct
     1321 ttggaactgt aaaccatgcc aacattagcg ccggtgaaat gaagggtatt aatcatgtaa
     1381 gagccgaggg taaccagcca gctcccccag ataaaatatt gcaaaaagga cattaccttt
     1441 aagcgcatcg cgatgctcat aatcgtttcc ttgtcgcatc atttggcctc acccgcgagg
     1501 ccgtttctta ttgtttttct caggcaattt tgcgcagaaa gccacaaata aggttgatga
     1561 agttctgctt agagagctcc gcagccgcca gcgtttgcgc atgtgacaat ttcacatcgc
     1621 ttaaaccttc cgccatattg gtaatcgcag agaccgcaac gactttaagg tcgcaatggc
     1681 gagctgaaat aacctcaggc accacagaca taccaacaac atccccacca ataatttgca
     1741 tcatgcgaat ttccgccgca gtctcgaaat tcggccccgg atacgagacg aacacgccct
     1801 ccgtcagagg gaaaccctct tctttcgcca ctttttgtaa cagtgcgcgg tattccgcat
     1861 cgtaggcatt tgccagtgag aagaagcgct ctccaaaacg atcatcgtta agacccacca
     1921 tcggcgtacc gggcatggtg ttgatatgat ctttcaatgc gaccaggctg cctgccccca
     1981 cttccggacg cagcgagcct gccgcattgg tgcagaacag tagctcgcag cccagcagct
     2041 taaaggtacg aattgcgtca gtcataatgg tcattccacg accttcgtag aaatgtccgc
     2101 gacctttcat acataccacc ggtacgccct ggagatggcc cagcactaac tcccccgcat
     2161 gaccatgtac cgtacttacc gggaagcccg gcagcttttc gtaggaaatt gcgacagcgt
     2221 tctcaatctg atcggccagc gcgccgagcc cggaacctaa aataaaggcc actcgtggcg
     2281 tgaaatcagg tttataagtc ttgataatat ctatgcaaaa cagcgggtta tgagaaaatt
     2341 gaacctgaga catacatatc ctttttctgt agggtggaat ctaacgcctt tttatagcga
     2401 ggttgtcccg gctcttacca atacactttt ctcacgcatt caatagcgat tcgatatcga
     2461 tggatttata tcttttctct attaatccac ggtaaaaact gtattggcga tattaattcc
     2521 tttgcattaa caatatgtcg tttttagcaa accactttat taagcaatct actaataacg
     2581 atcggcatag ggaattatgc tatgaaaaaa catcttttaa ctctgacact ttcctctata
     2641 ttagcgatac ctgttgtatc tcatgcagaa tttaaaggcg gttttgcaga tatcggcgtg
     2701 cattatctgg actggaccag ccggactacc gaaaaatcgt cgaccaaatc acacaaagat
     2761 gattttggtt atcttgaatt tgaaggtggc gctaacttta gttggggaga aatgtacggg
     2821 ttcttcgact gggaaaactt ctataacggt cgccataata aaccaggtag cgagcagcgt
     2881 tataccttta aaaataccaa ccgtatttac ctgggtgaca ccggatttaa tctctacttg
     2941 catgcgtatg gcacctacgg ttctgcgaat cgcgtgaatt tccacgacga tatgttcctg
     3001 tacggtatcg gctacaattt caccggcagc ggttggtggt tcaaaccgtt ctttgctaag
     3061 cgttatacag atcaaaccta ttacactggc gacaacggct atgtcgccgg ctgggttgca
     3121 ggttacaact ttatgctggg cagcgagaaa ttcactctta ccaactggaa cgagtacgag
     3181 tttgaccgtg acgctaccta tgcagcgggt aatggcggta aagaagggct gaatggcgca
     3241 gttgcactgt ggtggaatgc aacatcacac attactacgg ggattcagta tcgatatgcg
     3301 gatgacaaac tgggcgaaga tttttaccag gatgcgataa tctactccat caaatttaac
     3361 ttctgattga ttactgccgg ggctcccggc agtatgaatt cactctatat ctgatacaac
     3421 ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     3481 gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     3541 acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg tagcgccagt
     3601 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt
     3661 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     3721 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     3781 ggtacataat cttcgacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     3841 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag
     3901 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     3961 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     4021 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     4081 gtatcgcgca acgtgctgtt cagttcatcc agcagtcgct caagacgcaa agccagctgc
     4141 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     4201 tgattttcca gttccttcac actgtgactc actgccgact ggctcaggcc gatacgctct
     4261 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
     4321 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     4381 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
     4441 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     4501 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gcgattgccc tgctgttctt
     4561 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     4621 gcatttgtgg gtaatttgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc
     4681 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat
     4741 tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     4801 ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt caccattgct
     4861 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     4921 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
     4981 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     5041 ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
     5101 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
     5161 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     5221 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     5281 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     5341 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     5401 cagcaccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     5461 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     5521 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     5581 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     5641 tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct
     5701 tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc ttcgacacgc
     5761 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgtgacatct
     5821 gacttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
     5881 cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     5941 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
     6001 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
     6061 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
     6121 agcgagcaaa ggtggtttct ttcgcttttt ctagcgcgtt aaccacgttt tgtgccgatt
     6181 ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg
     6241 gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa
     6301 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gctccgcaaa
     6361 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
     6421 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     6481 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
     6541 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
     6601 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     6661 cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta
     6721 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga
     6781 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
     6841 gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     6901 acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     6961 ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
     7021 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg
     7081 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     7141 gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaata gcgcgaatag
     7201 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga
     7261 ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc
     7321 accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa
     7381 agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc
     7441 tgaaagccgc cagcggcgca gcgcctacac gttgggtagg cgaatcaggc gtaatcagtt
     7501 ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
     7561 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
     7621 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
     7681 caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
     7741 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
     7801 tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
     7861 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatggt gacacccgga
     7921 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
     7981 aataacgccg ggccgctacc atccgggcta agatgacgat ggtaaatatt catatcgcca
     8041 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
     8101 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
     8161 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
     8221 gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc
     8281 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg ctgcaccggc
     8341 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
     8401 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
     8461 gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
     8521 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
     8581 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
     8641 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
     8701 tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact
     8761 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
     8821 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
     8881 acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg
     8941 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
     9001 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
     9061 ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc
     9121 atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc
     9181 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
     9241 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
     9301 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
     9361 aaatttgcct ggtatctgcc gcgcgcaatt gtattgctaa ttctttactt aatccctggt
     9421 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
     9481 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
     9541 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
     9601 atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg
     9661 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
     9721 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
     9781 atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
     9841 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
     9901 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
     9961 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
    10021 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
    10081 tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
    10141 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
    10201 gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa
    10261 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
    10321 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
    10381 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
    10441 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
    10501 cgattctcca gttatcgccc aggcactggc tggtgaagag attaaacctg gcccgcataa
    10561 aattcagggt attggcgctg gttttatccc agctaacctc gatctcaagc tggtcgataa
    10621 agtcattggc atcaccaatg aagaagcgat ttctaccgca cgtcgtctga tggaagaaga
    10681 aggtattctt gcaggtatct cttctggagc agctgttgct gcggcgttga aactacaaga
    10741 agatgaaagc tttaccaaca agaatattgt ggttattcta ccgtcatcgg gtgagcgtta
    10801 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaactgcaac agtaatgcca
    10861 gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
    10921 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
    10981 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
    11041 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
    11101 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc
    11161 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
    11221 ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg
    11281 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
    11341 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
    11401 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt
    11461 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
    11521 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
    11581 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
    11641 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
    11701 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
    11761 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
    11821 aagataagca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
    11881 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
    11941 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
    12001 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
    12061 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
    12121 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
    12181 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
    12241 aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
    12301 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtgtgcgcta
    12361 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
    12421 tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    12481 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
    12541 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
    12601 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc
    12661 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
    12721 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    12781 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    12841 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    12901 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    12961 accaaccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    13021 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    13081 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    13141 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    13201 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    13261 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    13321 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    13381 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    13441 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    13501 gacggcacca ttggtaaaat ctttgaaacc aaccacgcgt tctctatcga atctgatagc
    13561 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    13621 aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    13681 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    13741 gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt
    13801 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt
    13861 tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc
    13921 atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc cttccctttc
    13981 agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt
    14041 acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc
    14101 tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc
    14161 agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt
    14221 tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac
    14281 gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg
    14341 accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt
    14401 ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca
    14461 tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat cgcaccaccg
    14521 tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc
    14581 agaccgttct gtttgatagc aggcacggca atgctgttgc ccacgctgcc gtaaaccacc
    14641 tgcgactgca cggcgacgat atccgcctgc agcgccctgc tcttatcgtt aaacaacaac
    14701 aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact
    14761 ttacctgatt ctggcagtca aatcggctat cacaaaacaa ggataaggta attcaatgaa
    14821 gaaaatcatt tgtctggtca ttacactact aatgacactc cccgcttacg cgaagttaac
    14881 tgcccatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt
    14941 gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttctc atctgcgtct
    15001 gaagctgggc aatacccgta accgcattga cactgccgag cagtttattg ataaggttgc
    15061 ttcgtcgtca tcgattaccg ggaagccgta tattgtgaag atccccggta agagcgatga
    15121 gaacgcacag ccttttttac atgcgctaat tgcgcagacg gataaaacgg tgcctgcgca
    15181 ataatcttaa atccccgccc cctggctaaa atgctcttcc ccaaacaccc cggtggaaag
    15241 gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc
    15301 cacccgcagt gctccggcaa ccgcgccgcc ggagctgacg ccacagaata ttccttcccg
    15361 cactgccagt tcgcgcatgg tgttttctgc atcgcgctga tgaatatcca gcacctcatc
    15421 caccagagaa gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgccggg
    15481 aatactgctg ccttcttccg gctgcaggcc aacaatcgtt actggtttgg attgttcgcg
    15541 cataaagcgt gaaacgccgg tgatagtgcc ggttgttccc atgctggaga caaaatgggt
    15601 aatgcgcccg ccggtttgct gccagatttc cggcccggta gtggtgtaat gggcgtaagg
    15661 gttatcggga ttattaaact gatcgagcag ctttccttcg ccacgattcg ccatctccag
    15721 cgccagatcg cgcgcacctt ccatgccctg ctctttggtg acaagaatca gttccgcacc
    15781 ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat
    15841 gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc
    15901 ttcgattaag acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga
    15961 aagtgccgca cgatctttca ccgaacctgc cgggttattg ccttccagtt ttaaccacac
    16021 ttcactgccg ttatccggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt
    16081 ttgttctaat gtactcacga tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg
    16141 cgtcttatca ggtctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc
    16201 gcctccgggc gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct
    16261 catcgcgggt ttcgatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga
    16321 ataaacgctc gccacgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt
    16381 tgtaccaccc cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca
    16441 gtacctgtac cggcagcggc gaatcgagac tggtacggcg gctgatatcc acttcccacg
    16501 ggcgcaggaa gagatccacc ggcccctgat aagcaggtgt gtagcccagc ggccagcgat
    16561 gtgcgccaac atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca
    16621 taaattcgag tacaaaacgg gtcgctggtt cgcgccatac ctgattcggc gcatcagcct
    16681 gttcaatatt cccctgactc atcaccacta cacgatcggc tacttcggtc gcctcttcct
    16741 ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc atgcagttga cgcagccagc
    16801 gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa cggttcatca agcagcagaa
    16861 tttgcggttc tacagccagc gcgcgcgcca gcgccacacg ctgtttctgg ccgccggaaa
    16921 gctgcgccgg ataacgatcc gccagatgcg caagctggac catttccagc aattttgtca
    16981 ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa
    17041 aagcgatatt gtcgaatacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac
    17101 cgaccttgcg atcgcgcgcg tgcaggcggc tgacgtcggt gccgtggaag cgaatatgcc
    17161 cgctggtttg atgctccagc ccggcgataa tgcgcagcag cgtggttttc ccggaaccgg
    17221 acggccccag caacgcgacc atctgacctg aaggaatatc cagtgagata tcgttcagca
    17281 cctgggtgcg accaaacgac ttcttaatat tggcaatctc aatgctcatg atgttcctcc
    17341 tgctgtgcgc gtttttcctg attctccagg cgccactgca acatactttt taaaaacagg
    17401 gtgataatcg ccatcagcgt taacagtgcc gcagcggtaa aggagccgac ggtgttgtag
    17461 tcctgctcca gcaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg
    17521 gaaaccaccg acactgcgcc aaactcgcca atcgcgcggg cgttggtcag taccacgcca
    17581 taaagcagcg cccagcgaat attcggcaat gtgacgcgac ggaacatctg ccagccagac
    17641 gcgccaagca aaatcgccgc ttcatcctcc tggctgccct ggcttaacat caccggcacc
    17701 agctcgcgca ccacaaacgg acacgtcaca aagatggtga ccagcaccat tccgggccag
    17761 gagaacataa tttgcagatt atgttcatcc agccagccgc ccagcgggcc gttagagccg
    17821 tagaacagca gatacaccag accggcaacc accggcgata cggcaaacgg aatgtccagt
    17881 agcgtcagca gtaactggcg tccagggaag ttaaagcgcg tcaccagcca ggccagcaga
    17941 atgccgaaca ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg
    18001 gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg gcatcagccc cttgctgaat
    18061 gcctgcacga agatgtaaat catcggcacc agcaggatga acgccgaaac cagcatcccg
    18121 atgccaatca gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgg
    18181 gtaacttccg ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa
    18241 ttgagaacag cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgctgccg
    18301 ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg
    18361 cgatatttcc ggcgataaaa atcaccgcgc caaattcacc aagactacgg gtaaacgaca
    18421 gcgccacgcc cgccaccagc gccggagaaa gctccggcag caccactttg cagaaactct
    18481 gccagcgcgt tgcaccaagc gtttccgccg cttcttcata ttccgggcct aactcttcca
    18541 gcaccggctg cacggtacgt accacaaacg gaatgctggt aaaggccatc gccaccgcaa
    18601 tccccagcca ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac
    18661 cgtttacaga aaagagcgag gctagcgtta aaccggcgac tgccgttggc agcgcaaagg
    18721 gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa gcgatagcgg gttaagatcc
    18781 acgccatcag caggccaaaa acgccgttaa aaatcgatgc cacaaacgct gacagcagcg
    18841 ttactttgta ggccgctact acctgcgggt tggtgatcac ctcccagtac tgcgcccagc
    18901 tcatctgagc cagctgcatc accagcgcgg agagcggcag cagcaaaatc aggcacacaa
    18961 acagcagact ggtgccgagg cttaaggtaa agcccggcag cacgcgtctt gatgaaactg
    19021 caaacattaa ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg
    19081 ggttttcatc acttccggcc aggagccaaa tttgtcttcc acgcggaaca gctcggtctg
    19141 cgggaatttg tttttcagtt tgtccatgac ttccgggtta ttcacacgat agtaatagtc
    19201 ggtgatgatg gtctgcgcct gtgggctgta gagccagttc aggtaggctt ttgcggcttt
    19261 ttccgtaccg ttggcctgca catttttatc gacccacgcc accgggaatt ccgccagaat
    19321 attggttttc gggatcacca cttcaaagcc ctgtgcttca tactgtttac ggatgttgtt
    19381 cacttccgat tcaaagctaa tcagcacatc gcccaggcca cgctcggcaa aagtggtggt
    19441 tgcgccacga ccgccggtat cgaacacttc aacgtttttc aggaactggg tcataaactg
    19501 ttcggttttg cctttgtcac caccgtcagc tttatccgct gcgccccatg ccgccagata
    19561 ggtataacgc gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg
    19621 caccaggtcg ttccaatcgt ggatattctt cgggttaccc ttacgcacca ggaagcccat
    19681 ggtggagtag aacggcgagc tattattcgg caggcgcgac tgccagtcgg ccgggatcag
    19741 cttgccttta tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtcggc
    19801 ttttaagccc tgtaaaatcg ccagcgcctg ttttgatgac ccggcatgag attgttttat
    19861 cgtcagtttg tcgccgccgt tatcttttgc ccattgttgc tcaaacggcg gattcagggc
    19921 ggcaaacagc tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg
    19981 gcccgccagc agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc
    20041 cattgcgcac ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt
    20101 gaaaggagta acggttttat ataccgtttg gtgatttgga agttgaaaag ggaataagaa
    20161 ccgggtttaa aacgaaatcc tccccacaaa tgcagggagg aaacagaggt gaatcagata
    20221 ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt
    20281 aaatagctgg attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg
    20341 gcgaggcgac gcatcgggat tgctttcgcc atttcagtca gcaccgactc tggatcttcc
    20401 gggttcgact ggcgggcaat gctttccgcc attggtgtgc gcacgtatcc cgggcaaatg
    20461 gcgttaacgc gaataccaga ctgcgcgtac tccaccgcca gcgattttgt caggccaaca
    20521 atcgccgctt tcgttaaggc gtacgccgtt tcgccaggat cggccaccat atcaccagtg
    20581 actgaagaca tcatcacaat gcgaccatct ttgcgcgcaa tcatctctgg cagcaccgcc
    20641 ttcgtgacgt tccatacgcc tttaatattg atgtcaatat ggaaatcgcg atcttcatcg
    20701 ctcatatcga ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg
    20761 cgcccttctt tttccttcgc gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca
    20821 tcggcgacaa ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc cagcttttcg
    20881 atctcagggg agatatccag caagattagg tttgcgccat gacgcgcaaa agttctggca
    20941 attccttcgc caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta
    21001 cccattttaa tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac
    21061 aggataaaca aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc
    21121 gctgctttat gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca
    21181 atgactcgac atcattgagt tgcacaagac caacaaacag taaatcggta acagaatttt
    21241 gcgccgtgcg ggtagacatc gacgagctac gccattcggt ttcaccggaa acggtatcga
    21301 gcgtgaaatg cgccagccgc cgcagcggag agtcagccag agaagtaatg gcaatcaccg
    21361 ttgccccctg ctttcgtgcc gcttctgcgc acaagacaat ctctttttta ctgccgctgt
    21421 aagagatagc gatttgtaca tcgccttttt tcaacgcctg ggaaacggtg gcttgtacgt
    21481 gcgtgtcggc ttcgcaggcc acgcgatagc caattttcat cagtttgaac gacagatcgc
    21541 gccccaccag cgccgatccg cccaggcccg ttatctgaat aaattgcgct ttactgatga
    21601 catcaatgat tttttgcagc cgcgcgtagt cgaacaacgc gcaggtttgc tccagcgcca
    21661 gctctttttc gcgattcagt ttgcgggcaa tcacttccag cgaatcatca ctggtaatcg
    21721 aactgtgcag gtgcagcgcc gtggcattcg ttttctcccg gctggcactg tattcgccaa
    21781 ttaacgccat acgcaattca gtaaatcctt gcgcgccgag cttctgggca aacttcacaa
    21841 tactcgactg gctgatgccg agctgtttcg ccatcttgcg cgaagagacg gatttcagct
    21901 cactcacgtt ggcccgcaga aagtcagcaa ttttttgctc gttctcggta aattcacttt
    21961 ccgcattgcg tattttcgtc aggtacaaca tcttccactc tcctgggctg gcagcaaaaa
    22021 ttccgcttaa gttcttatga cgctcttcac actctgcgag tgtagactca atgattcctt
    22081 taagtctgtc aattcgggat atatgattcc atacatctgc aattatttga tcgtaaatag
    22141 taaggtcacc accgatgcaa cttgaaaaga tgattaccga aggctcgaac gccgcctcgg
    22201 ctgaaattga ccgcgtatca acgctggaaa tgtgccggat tatcaacgat gaagataaaa
    22261 ccgtaccgct tgccgttgag cgcgtactgc cggatatcgc cgcggcgatc gatgttatcc
    22321 acgcccaggt aagcggcggc ggtcgtctga tttatctcgg tgcgggaaca tccggtcgtc
    22381 tggggattct ggatgccagc gaatgtccgc ccacctacgg cgtgaaaccg ggtttggtgg
    22441 ttggtttgat tgctggaggc gaatatgcca ttcagcatgc ggtggaaggc gctgaagata
    22501 gccgggaagg tggtgttaac gatctgaaaa acatcggctt aaccgcgcag gatgtggtgg
    22561 ttggcattgc ggccagtggt cgcacgccgt atgtaattgc cggactggaa tacgcacgcc
    22621 agctcggctg ccgcacagtg ggaatttcct gtaatccggg gagcgccgtt tcaaccaccg
    22681 cagagtttgc cattacaccg attgtaggtg ccgaagtggt taccggttct tcgcggatga
    22741 aagcaggtac agcgcagaaa ctggtgctca atatgctctc caccgggctg atgattaaat
    22801 ccggcaaagt gttcggcaac ctgatggtcg atgtggtcgc caccaacgaa aaactgcatg
    22861 tgcgccaggt caatatcgtt aaaaacgcca ccggatgtaa tgccgaacaa gcggaagcgg
    22921 cgttaattgc ctgcgagcgc aactgtaaaa cggccattgt gatggtgctg aaaaatctcg
    22981 atgccgcaga agctaaaaaa cgcctggatc aacacggcgg ctttattcgt caggttttag
    23041 acaaggaata acccatggcc aaagagatca gcagtgaact tctgaacacc attcttaccc
    23101 gtgtcggcgg accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg
    23161 gtgtacatga cagttcactg gttgatccca atatcaaaac gctggaaggc gtgaagggcg
    23221 tcattttgac cagcgaccag gtgcaggtcg tttttgggcc tggtaaagcg catcgtgccg
    23281 caaaagcgat gagcgagctg ctgggcgagg caccggtaca ggatgccgca gaaatcgccg
    23341 cccagaacaa acgtcagtta aaagccaaac aaacctccgg cgtgcaacaa tttctcgcca
    23401 aattcgccac catcttcacg ccgctgatcc ccggttttat tgccgccggt ctgctgctgg
    23461 ggatagcgac gttaattgcc acggtgatgc acgttccggc agatgctcag ggcacgctac
    23521 ccgacgcgct gaattttatg aaggtgttca gcaaaggttt gttcactttc ctggtgattc
    23581 tggtgggcta taacgccgcc caggcatttg gcggcacggg cgtcaatggc gcaattattg
    23641 ccgcactctt tttgctcggt tataaccccg ctgcaaccac cggttactac gccggttttc
    23701 acgatttctt tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ctgattgccg
    23761 cgtgggcctg cgcccgcatt gaaggcatgg tgcgccgctt tatgccggac gatctcgaca
    23821 tgctgctgac ctcgttaatc accctgctga tcaccgctac gcttgcgtac ctgatcatta
    23881 tgccgctggg tggctggtta ttcgaaggta tgtcatggct gtttatgcac ctgaacagta
    23941 atccgttcgg ttgtgcggtt ttagccgggc tgttcctgat cgccgtggta tttggcgtgc
    24001 atcagggctt tattcctgtt tacctcgcgt taatggacag ccagggattc aacagcttat
    24061 ttcccatcct ttcaatggca ggcgcgggcc aggtgggcgc ggcgctggca ctctactggc
    24121 gggcgcaacc gcacagtgca ttacgcagtc aggtacgcgg ggcgattatt cccggtctgc
    24181 tgggcgttgg tgaaccgttg atttacggcg tcaccctgcc ccgcatgaaa ccgtttatta
    24241 ccgcctgttt aggcggcgca gcgggcggtt tgtttatcgg cctgatagcc tggtggggtc
    24301 tgccgatggg cttaaacagc gcctttgggc cgtctggtct ggtggcgctg ccgctgatga
    24361 cttccgcaca aggcatcctt ccggcaatgg cggtttatgc tggcgggatt ctggtggcat
    24421 gggtttgcgg gtttattttc accacgctct ttggctgccg taacgtcaat ctggactgat
    24481 atcatgaaac ggacaatgct ctatctttct ctgctggctg ttagttgtag cgtcagcgcc
    24541 gcaaaatacc ctgttctgac agaaagttcg ccagagaaag cagggttcaa cgtcgaacgg
    24601 cttaaccaga tggatcgctg gattagccag caagtggacg ccggttatcc cggcgtaaac
    24661 ctgctgatca ttaaagataa tcagattgtg tatcgcaaag cctggggagc ggcgaaaaag
    24721 tacgatggca gcgtgttgat ggaacagcct gtcaaagcca ccaccgggac gctgtatgac
    24781 ctggcctcaa acaccaaaat gtacgccacg aacttcgccc tgcaaaagct gatgagcgaa
    24841 ggcaaactgc atcctgacga tcggattgcg aaatatattc cggggtttgc cgatagtcca
    24901 aatgacacca ttaaaggcaa aaacaccctg cggatttctg acctgctgca tcacagcggc
    24961 ggtttcccgg cagatccgca atacccgaat aaagcggtcg cgggcgcgtt atattcccag
    25021 gataaaggcc agacgctgga gatgatcaag cgcacgccgc tagagtatca gcccggcagc
    25081 aagcatattt acagtgatgt cgattatatg ctgcttggat ttatcgtcga gtcggtcacc
    25141 ggtcagccgc ttgaccgcta tgttgaagag tcgatttatc gcccgctcgg cctgacacat
    25201 acggtattta acccgctgct gaaaggcttt aaaccgcaac aaattgccgc cacggaattg
    25261 aacggcaata cccgcgatgg cgtgatccat ttcccgaata tccgcacctc cactctctgg
    25321 ggtcaggtgc acgatgaaaa agccttttat tcgatgggcg gcgtttccgg gcacgcaggt
    25381 ttgttttcca ataccggcga tattgcggtg ttaatgcaaa cgatgctgaa cggcgggggc
    25441 tatggcgatg tgcagctgtt cagtgcggaa acggtgaaga tgttcaccac cagctctaag
    25501 gaagatgcca cttttggcct cggctggcgc gtaaatggta atgccaccat gacgccgacg
    25561 tttggcacgc tggcaagccc gcaaacctac ggtcacactg gctggaccgg aacggtgacc
    25621 gttatcgatc cggtgaatca tatggcgatt gtgatgttaa gtaacaagcc acattcaccg
    25681 gttgccgatc cgcaaaagaa tcccaatatg ttcgaaagcg gtcagttgcc aattgcaact
    25741 tatggttggg tggttgatca ggtatatgcg gcgttaaagc agtaataaaa aggccagtca
    25801 gtaatgttac tgcctggcct ttcatcatcg aatcagaaac gcttacagcg ccatcaactt
    25861 gtccagcgac ggcgcgaagt aatagccgcc agtgaccggt ttggtgaaac gtaacatcgc
    25921 atcacgcttg ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag
    25981 acgcgcgcag taggcgcaga agtacagacc gtgagtgcca ctggcggtgc cgtacggcag
    26041 gctctggcga acaatcttca gtcctttgcc atcttctttc agatcaacgc gggtcaggtg
    26101 agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat
    26161 catcatctcc tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gttcccaacg
    26221 ctggacaaac acatagctgc cgcccgcatc cacgccgtct ttgataaccg ccacttcgcg
    26281 acgcgtctct tcacccgccg ggttttccgt accgtcaaca aagccgctca ggtcacgctc
    26341 ttcaacccaa cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc
    26401 catcgccgcc tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa
    26461 cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc
    26521 ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg ttaccaaagg caaccaccgc
    26581 accaagatgc gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt
    26641 actggccgca cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc
    26701 gcggcaatgt tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa
    26761 taatgccacg caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcggcggcg
    26821 gtttgttcag acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg
    26881 gtatcgtccg aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta
    26941 atatgttgac tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt
    27001 tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg
    27061 gtgtcagcag gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg
    27121 ttaatcacca tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg
    27181 ttcgcggttt tcattccgct gcgcaggcga taatcgccat caagcgcatc ggcaatggct
    27241 tgttcttgca gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg
    27301 gacgacccaa accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa
    27361 cagccggtca gcatcaatgg catagcgcat aacattaaac gcagcgattt catgtcactt
    27421 cctttggtta ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct
    27481 tttacttcat taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca
    27541 atatccgcca ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc
    27601 cacggttgcc agcaaagtaa aaccacctgc gccaacagca acagaaaata gagcacatgc
    27661 caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg
    27721 ccaggaatca gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca
    27781 gtaccctgct cacgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc
    27841 aatagcacaa gccagaagag caagggtaaa cgcaggcgtc cgtgcgcatc ataatggaca
    27901 ggatggaact cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc
    27961 gtgttcatat cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacatt
    28021 gatctgaatt ttcgggcagc cacgagcaat cagctttttc tccagccgat taagcaacgc
    28081 attggcaatc ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg
    28141 atgcccgtca taaccgccca tcaccgttcc gaccacttca ccgtttacct cagcgaccaa
    28201 aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg
    28261 acgcagcaag tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa
    28321 tacgcgtatc tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg
    28381 aacggtcgaa ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt
    28441 tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat caaaccgtcg taacggatta
    28501 cgcgatacga tataacatct ggaactttat tattacaact caggccgtat gagcactttt
    28561 aaaccactaa aaacactcac ttcgcgccgc caggtgctga aagccggttt ggctgccctg
    28621 acgttgtcag gaatgtcgca agccatcgcc aaagacgaac ctttaaaaac cagcaacgga
    28681 cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc
    28741 cacggcggaa ttgataccgg agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg
    28801 ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta
    28861 acgcgttctg gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat
    28921 ggcgcagatc tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt
    28981 gcttcggtat ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct
    29041 gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta
    29101 ttgcagcaag tgctgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctc
    29161 ggctcgcata ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa
    29221 caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg
    29281 tttatcacca acccggaaga agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc
    29341 gccacagcga ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca
    29401 cattcgaaaa agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc
    29461 aaccttcagg atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa
    29521 gatagttggc agcgcgaagc tggcggcggc gggcgtagtc gggtgttgcg taatggtggt
    29581 gttttcgaac aggcaggcgt caacttttct cacgtccacg gcgaggcgat gccagcttcc
    29641 gccaccgctc atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttcactg
    29701 gtagtgcatc cgcataaccc gtatgttccc accagccacg cgaatgtgcg tttttttatt
    29761 gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg gcggcttcga tttaacccct
    29821 ttctatggtt ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgcctg
    29881 ccatttggtg aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc
    29941 aaacatcgca acgaacagcg cggtattggc gggctgttct ttgatgatct gaacacgcca
    30001 gatttcgacc actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat
    30061 ttaccaattg tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag
    30121 ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cactctgttt
    30181 ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc
    30241 tgggagtatg attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt
    30301 aaggtcaggg attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc
    30361 aacgacggct tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag
    30421 aatccccact gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt
    30481 tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa
    30541 atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat gcagttgatt acacaagtcc
    30601 agcaccgtca ccggctcaga catattttcc agcacatatt cacgggcgcg ggaaagtaat
    30661 ctacggtaac tctgatgact gatgctttcc gccgtcacca ttggttgcgc gtcttccagc
    30721 atcgccccca tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg
    30781 agattttccg ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg
    30841 gctttatgct gctctttcac ttccagcgcc gactggctac gcagcatatg taatacccga
    30901 tccgggttat gcaaaaagtt agcctgtcgg gtgatgacat cttcagaaag caccacgccc
    30961 aggatcgtgt aatcatccgg cgtgctcagt tcaaattcag tgccaccagg gcgggtggcg
    31021 atttccgcgc ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga
    31081 atgccaaacc agaacgagtt cggccagacc aggcacgact gacgcagcgc cagaccggtg
    31141 tattcacgaa aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg
    31201 cccggatgca gctgatcgta aatctgctgc caggcggtaa tcgttaaagc atgttcatag
    31261 acatccgttg tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg
    31321 ttttcgggta agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg
    31381 atgttaatgc cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga
    31441 cgataacgac tactgcgttt acgtaacgtc ccggcagaaa agagctgttc gagcgcattt
    31501 ctggcttttt ccagcggcca gccaaagtgg gcagccactt ctcctgccgt cattccctga
    31561 cgtactgatg ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg
    31621 tcgggttccc ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg
    31681 tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac gcccggtacg catcgcggca
    31741 gcacaacctg cgtccagcgc cgcacgacac gccgccagat cgccttccac caccagcgtt
    31801 aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg
    31861 gcatccgctg catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg
    31921 atcatcggca acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca
    31981 tcggtaaagg cgttacaggc tgctttacac gcggcctggc taccggttaa aaatgctgcc
    32041 gagtagttgg tttcagacgg tggcgggaca taggtcacca gctgtacgtc ggcagatttc
    32101 aacgctgcat caatgccata ggtcgcttcc agcggcggtg ccaccagata cgccatcgga
    32161 tcgccaagcg tgatcccggc ggttgatgag agataagaac cggtacgcga aaccacatgc
    32221 gccaggaagg cggtgttttc tgcgtcattg gcccactgga atgctgcacc attttcaata
    32281 tgtgcaacca tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata
    32341 atcagcactt caccggcagt cggtgacggg ccgtgtgcag cgcccgcata cagcgagcga
    32401 ccatatacca cttccaccat cgcctgcttg gtcgcttcat cagcggcaat gtaggtgacg
    32461 tcatcggaat cagcagaaat cagcccgagg ctacgaatat gcggcggcaa ttttagttca
    32521 cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc
    32581 aaatctaaag ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc
    32641 tccagcatac gtttcgccaa atccacaatg acggctgcgg cttcaaccgg cggcgtgccg
    32701 ccctggtgaa tgttagagat acaggtgcga tcggcctcaa cggtggtcgc catacgcggc
    32761 gagtaaacgg cgtagcagga gaggctttct gactgcccta accccggacg ttcccccacc
    32821 agcaggatca ccactttcgc gccgaggatc tcaccgatct gatcttcaat cttcacgcga
    32881 ccataacgaa caaagaatgg cgtgccgact ttcagcccgg cctgtttcag gcccgccatc
    32941 agcggcggca ggatctcttc gtagttcacg gtgatggcat cagttgacag gccatcagaa
    33001 ataacgacct gtacgtccgg attagcaaca cactgtgctt tcagcgcttc aacagcttct
    33061 gcacacaggc ggcggcccat atccgggcga gtcaggtaga gatttttgtc gctaatttca
    33121 gagcgtactt ccagcaagcc ctgcgctttc acccactctt ccggcacttc tttcagtacg
    33181 gtatctttcg aacgagagtg atcggccagg aaacgcagca gcgcctgggt acgtggacgc
    33241 ggaccggcac gaccggtaca aacgcgggcc acggtgctgc ggcgcagttc tgttaatacg
    33301 tctgcgcgat gcgggttttc aacaccaatc cacgcttttg cttcagcgga acctaaatcc
    33361 agcgcacagc tttccgaggt caccggtgcc gcacagttgg tggtggcgca ctttgcttct
    33421 gacggcgcgg gggccgtttg tcccattgac gccatcacgc tgcgtacaat ttcttcaatc
    33481 tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg
    33541 cccgtttggt caggcgaccg tttgccataa tgcccatgct ttccagccag cgttcaaact
    33601 ccggtgacgg acgcaggttg agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg
    33661 tctggtagtt gagcatgatg tcatcaccca gcggcatccc catgatgtag ttgcagcctg
    33721 cggtggcgag caggatcatc aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg
    33781 tgtaacagca gtcacagccc atagagatgc cgctcagctt gcccataaag tgatcttcta
    33841 agccagcacg gataatctgg cggtcgttgt agagatactc cggcccaata aagccgacca
    33901 cggtgttgac gataaacgga tcgtaatgac gcgccagccc atagttacgt gcttccatcg
    33961 tcacctggtc agcgccgaag ttagcgccag cggatagcgc agagccttgt ccggtttcga
    34021 agtagaggca gttttccccg gcgatacggt tgaactctgc acccactgcg cgcgcttcgt
    34081 cgagcatcgc cagttccacg ccaaactctt tcagcccttt ttcgctgcca cagatactct
    34141 ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg
    34201 ccagtacgca gccctgagtt gggatgttga atttgtcgat cacgccgtaa atggtgtcca
    34261 acacgcggct taagttttcc acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc
    34321 ccaccccgaa ggaaagccct tcgtagattt gcgcggcgat actttgcacg tcgtcacggg
    34381 tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat accaatggtg gtattggcct
    34441 ttttgattac cggcattttc ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg
    34501 ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc
    34561 tggtttcatc gctcagcaca tactcacgca gttcgctgat gctccagttt ttaatctggt
    34621 tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg
    34681 caatcaccgg attattgcgg atgtccgcta cggtcatttc cgacaacacc tgctttgccg
    34741 ccacgcgctc ctgtgaactt gccgctgcaa cgcctgccag cacatccccc gaacgcagtt
    34801 cgttggcttt agccagcacc tcttttacat ccttaaactg atatacattg ctgaacaatg
    34861 tggtctttag tttcataagt cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac
    34921 cggcacaacc gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac
    34981 aatcacttcg tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acaacatgcc
    35041 cagagcttta ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg
    35101 cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag
    35161 cgaggcggga agcgccagca cgtacgcgtc agttttggga tcaagatcca gctgaagcag
    35221 cgcctgttgc caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt
    35281 gcgcagcggc agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg
    35341 tgcacccgcg ccaatcaccg tggcgcgtac ggtttgcgcc ggaaactgca cattcatctc
    35401 acgcaggcgc ggatggtcat gcagcgccgt tgccagaagt gggccaatat cggcaaaaca
    35461 gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg cctccagaaa gcgtaatgat
    35521 ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc aatgcctgcg cgagcggcga
    35581 aagcgttccg tcaatcactt cgacaatcag cgccgccatc cgccgggtaa cctgcaccag
    35641 ctgcgcgccg gtcagcgaac gggcgtcagt gcctgcaccg aagcactcat ccacaatcat
    35701 ctgccccggt ttatgagcgt aaaccacgcg cccctggctg tcggtttcca acaggcgacc
    35761 accgacgttg agacaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc
    35821 ggtgccaccg ccgatgtcga tattcagtac ccgacacagc cgttgttcag aaagggtttg
    35881 tgccccggct ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcaacgac
    35941 aaaatcgccc agcgactgag agagcgccat caccgccggg cgagcattgc gggttttcgc
    36001 gctttcaccg gtgatgatga tggcaccaga atcaacgctt tccggcgcaa tgcccgcagc
    36061 ctgatattgc tctaaaatca gcgatttcag ttccgcttct tttaaaccac cctgtttatc
    36121 gacaggggta aagaataccg ggctttgcca gctaatttcg cgtttaatga attcgtagcg
    36181 cggcacctgc gacaccgccg cacggttaac cagttccagg tgggagaaaa tcacctgggt
    36241 ggtggtggtg ccgatatcga taccgacgct caatagctgg cgagtgttca cgattgtgcc
    36301 tccgcttcgg ttttagtcgc ggtcgcgtct tcttttggca ccagcatcat cgccacgcca
    36361 atcgccgtta cgccgccgat caacttgccg acaatcatcg ggaagatcat ggcgttcatg
    36421 ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg
    36481 cagttgatga ctttgccgcg ggtatccatc tgcttcatca tgccgaacat cgggatgttg
    36541 ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt tcatattcag tactttaccg
    36601 acgctcatca gcggtttttc aaaccagcga gtcagcagca gcaccattgg atacgcccct
    36661 aacagcacgc aggagataga accgataact tcaatggcgc gcatcacttc accgggttta
    36721 tcgccagggg ccataaagat aggatccagt ccggggatca gttcccagcc aaggaggaat
    36781 ttcactaccg cagcggcaag accgagggtg atcaatgcaa cgaggaattt ggcaaagatc
    36841 tggaagccgt tgatcatttt ttccgggatg aatttcagcc ccagcgccac cagaatcgcg
    36901 acaatgatca ccgggatcat gttcatcagg atcagggcga aagtgaattc caccggctga
    36961 ccgttgatct gcacaccgga gtacatagca accagaccac cagcaataca gccaatcgga
    37021 atggtcacaa tgcccgccag cacgccgagc gccagataac gacgatcaga aggttcgata
    37081 atgccgagcg ccaccggaat ggaaaacaca atcgttggcc ccatcatcga cccgagaatt
    37141 aacccagagt atagccacgc tgctacgtcg ccgcccgcca gctctttggc gaggaagaag
    37201 ccgcccatat cgcacgccag cagtgttccg gcgaacattg acgggtttgc gccgagcatt
    37261 tcgtaaaccg ggataattac cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc
    37321 ataccgacca tcgccagacc cagagcgccc attgccataa agccttcttc gaactgaccg
    37381 cctgatcctt cgatactttt accgaactta ccgaggaaac gagcagaacc gccgaactgc
    37441 gacaggatcc tgtctacggc agctatcagc ataaagaaca tcatgatgta catgatgatt
    37501 tcgttaattc ccatcgcctt tactccctgt tagttgttat ttattggcgg atgcggcgta
    37561 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcgtcg
    37621 catccggcat ttattgcgcc gccgcgtaca gtccgacaat ttgttccagg ctggcggtac
    37681 gcgggttgct gcgcagacaa atatcttcct gcgcggcctg cgcccatgca ccgtaatgcg
    37741 cagatgtcgc gccaacatcg cccagtcgtt taccaatccc aacttccgca atcagctcac
    37801 ttaccgcgtt aatagcgtca cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct
    37861 gactaaaccg ttcgcgacaa accatccggt taaattccat caccgttggc agcaacatgg
    37921 cgttcgccag accgtgtgga atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc
    37981 acaacccaag acccgcactg gaaaacgcca ttcccgccat acatgaagcc agcaacatgc
    38041 tctcgcgcgc ggcaaggtcg tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca
    38101 tcgcaatggc accaatcgcc agactgtcgg taaacggtgt agcgttcagg gcgctgtatg
    38161 cttcaatggc atgggttaac gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac
    38221 cttcggtcaa tgcggcgtcg aggatcgcca catccggcat cagcgaggca tgggctaaca
    38281 cctgcttgcg cccgctcacc gcgtcgataa tcaccgttac attggtggtt tcagagccgg
    38341 ttccggcggt agttggaatg gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg
    38401 acatctctgc cagcgtgcta tccgggttcg tcaccagcaa cgccacggct ttcgccgcat
    38461 ccagcaccga gccgccgcca aatgcgatca ccccgtcaca acctgactca cgcaactgcg
    38521 ccacggctgc acacacgtcg gtaatgcacg gttcgcccac cggacatagc cagagcgtca
    38581 tggcgatgcc tttaacggcc aggctgcgcg tcagcccggc ggtcatccct gcctgatgca
    38641 aaaagctgtc tgccatcacg aacagatgtt tcagcccacg cgtttgcgct tgctgcccgc
    38701 aactgctcac cgcgcccaga ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc
    38761 gttgcagatt cagggtatcg aacgcctgaa agagcgcggt ctgcaattca ttttgcatat
    38821 agcccctccg ctttctctcg cccactactg gcgatcgcca gcggggtcat aaacaaactg
    38881 tgctgcggta aatgcacctg taacgccggg aattgtttgc gaaacagttc tgccacgccc
    38941 ggttgcatac aggagccgcc cgccagccat aaatcagtaa tgccctgccc ttcaatatgc
    39001 cgggcgacaa tgtccgccat tttttcgtac accggtttca ccgcaggcca aatctcgtca
    39061 ccgtgaccgc gcttgtactg ctccgcctct tccagcgaaa tacggcgatt tccggcgaga
    39121 gtcagagaga tgtgatgtcc gccggtggct tcatccgccg agtacgtcac tttgcccttt
    39181 ttcacgatgg caatgccggt agtgccaccg ccgatatcca ctacgcctgc gttatccagt
    39241 tgcagtagat ccgcgactgc ggttggctca tccagcacat ggctcacttc cagcccggca
    39301 gactccagca cgttgatgga aatacgcggg tccgtacctg gaggaaatga agtcgccgca
    39361 tggctaaaac ggcgaccgaa ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg
    39421 acagcgccga agaaatccca gacgatgccg tcgcggacca catcggccca gtcgaggcat
    39481 accgccaccg gctgaccgtc gcggtcgaca accatcgaca ccacatcgca ggtgcccaga
    39541 tccaccccca gccacagcgg agattccgtc gcggcgggcg tctggttaca cagcgttgcc
    39601 gccgtttgca gacgtggggt gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac
    39661 aatgcgaaac gcatcgacta atacacagcg acgcagacgg acaaacgtac gcgcgctggt
    39721 taccccttca ccggttggcg tggtgatggt catggtggtc cagccttccc cgcccagccc
    39781 cagcccggca atgcacggtc cgttcttaac gaaaatgctg gtatcaatag cattcgccat
    39841 ctggttcatg ttttcgatgt tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc
    39901 cagtttcacc gccagcgcaa tggcatccgc cacgttggcg acgcgcacga cgggcagcac
    39961 cggcatcatc agttcggtca cggcaaacgg atgttctgca gtggtttcca caaacagcaa
    40021 acgcgtttct tgcggcacgt tcaggccgat tgccgccgcg attttcgccg catcgcgacc
    40081 aacccagtca cggctgacgg tgccttttcc gcgctcgtcg atatttttca gcaacaccgg
    40141 ttgcagctgc tgcgcctgtt ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg
    40201 catcagttca tcggctacgc tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat
    40261 gttgttatcg aaagacgcgc ctttgacgat ggactgagca gcacgggcga ggtcagcggt
    40321 ttcatccacc actaccggcg ggttgccagc gcctgcggca atcagacgtt tattggtgtg
    40381 tttacgcgcc gcttctacta ccgcttcgcc gccggttacc accagcaggc cgatacccgg
    40441 aaacttgaac aagcgttgcg cggtttcgat atccggattt gccacagtaa ccagtaagtt
    40501 ttccggccca cctgcggcaa caatcgcctg gttgagcagc gtaatcgccc gctgggagac
    40561 ttttttcgcc gccggatgcg gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat
    40621 ggcgttatta attacggttg ccgccgggtt agtggacgga gttaccgaag ccaccacgcc
    40681 ccagggcgcg ttttcaatta aagtcaggcc gttgtcgccg gtcagcacct gcggagagag
    40741 acactcaacg cctggcgtgc cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac
    40801 gcggcccatg ccggtttcac tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc
    40861 tgcctcacga atggcagcaa tggctaactg gcgcattgcc acgcttttta acccttgctg
    40921 ggcgactttg gctgccgcaa cggcgtcatc cagggacgcg aaaacgccca tctcatgaac
    40981 ggcggcggac ggcgtgtcac tgctttgcat tttcagcagt accgctttca ccacctgttc
    41041 aatatcctgt tgattcatga tgttctgcct tatttgtgga aaattacctg accgccagac
    41101 accacctcat cgacaatgcc aatcacgcac agatcgaccg gtgacgtttc gcttttatgc
    41161 gcctggcggg cggaactgcc actcaccagt aacacccact ccccggttcc cgcgccaata
    41221 ttgtcgatgg cgacggcgca ttgcccgtca ggattacctt gtggatcaat catttccacc
    41281 atcagcaatt tgtcatgcgc cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg
    41341 acgactgcca gtttcatacc cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc
    41401 tccgcagaag gtaaaatgaa aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg
    41461 tgattacagg ttgctgctat cgcctttcag gccgatcggg aagacttctt ccaggtcgcc
    41521 gtgcggacgc gggataacgt gtacagatac cagctcgccg atacgctgtg ccgctgcggc
    41581 acctgcatcg gtcgcggctt tacaggctgc aacatcacca cgaaccatgg cagtacacag
    41641 gccgccgcca atctgcttaa caccaaccag cttgacgcgc gcagctttca ccatcgcgtc
    41701 agaagcctca atcagtgcaa ccaggccccg ggtttcgatc attcctaatg cttccattgt
    41761 gctttcctct ttatcagggt ccagaacggg accgttcatt caaccagtgt ttgtaaactg
    41821 ctttcgcggt tcacttctgt ctgacgcggc acagctgcca ccagcgccag ctcgataatt
    41881 tcctgcacgc tacaaccacg agagagatcg tgcatcggcg cggcaagtcc ttgtatcagt
    41941 ggcccgacgg cacgatatcc gccgagtcgt tgtgcgattt tgtaaccaat atttccggct
    42001 tccagcgacg gaaaaaccat cacattggcc ttgccctgta gcgggctggc aggcgctttt
    42061 tgcgccgcca cttccggcac gaaggcggcg tcaaactgta actcgccatc caccaccagc
    42121 tttggtgcgc gctcacggac gatttctgtc gcctgctgga cgttagcaac acaggggtga
    42181 cgggcgctac cattgctgga aaacgacagc atcgccacgc gcggctcttc tccggtgatg
    42241 gcgcgccagg tttcggcact ggcaagcgcg atatccgcca gctgcgccgc cgtcggctgt
    42301 ggcaccacgc tgcaatcggc aaagcccaac ggcgggccgc tgtactgtgg caacatcagg
    42361 aaaatggaag agagcgtttt acagcctggc tgcaagccga taatgcgtaa tccggcacgc
    42421 agcacatttg ccgtggaaga gaggttgccc gcgatacaga catccgcttt accggcgctg
    42481 accattgctg cggcgaacat cagagggtcg gtaagttttt ccagcgcatc cggcggcgtt
    42541 ttttcgcccg cgcgggccag ccagcgatga gcaaattctt cccgcattgc gaggttgcca
    42601 tgcggatcaa tcacctgtag cccgtccatc gccacgccgt gactgagcgc aaactgacga
    42661 agttcaaacg gattggcgac cagaatgggc gttgccagac cttgttgatg taaatattgc
    42721 gcggctttca gcacacgttg gtctaacgca tccggaaaaa ccactctggc gggcgctcgc
    42781 aacgccagtt cacgacaacg ttcaataatc attgcgtttc ccccagtcgt tgttggatct
    42841 gcttcaccgt tagcggctgc ttcgttacgc tcaggatcat catcacgtag accgtgctgg
    42901 aaaggcggtt cagcgcctgc aaaatgtccg ggcgtaatac ttcaaagctg cgggtgataa
    42961 acacctgcgc cgctacggtt tcggtttccc gcactttggt acgtagcaga ttgagcaacg
    43021 ccgcatcgcg cccgtggctg gcttccggca ccagatgatc gtggtcgaga tagcgcagcg
    43081 gctggtgaga aagtcgatgc agatcttcgt cagaaagccc gacaatcgcc tggcagccca
    43141 gcggttcacc cagcgcatcg gcgcgcataa tgttgccaag acgcgaacgg atatccgcca
    43201 gccacggctg ccacggttcc gccagttcaa tttgcagcca cactgccagc gcaatggtgc
    43261 tgtcgagaac cgcgcgaaaa cccagacgcg gatcgctttt ggcgaccatt ttttccgccg
    43321 acaggtgagt cagcgtatcc ggcttttttg ccaccggctg gcggcacagt tcgcagcacg
    43381 cctgcggatg ttcatcgcta ctggtcagcc catgaacggg ctgcggctgc tgctgttcat
    43441 cgtcaacaaa caggcggccc tgctcgtcaa taaacttgat gcgcagatgg cggctttcca
    43501 gtaactcccg ggcagagggc gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt
    43561 cgctgagcgt atggttcgct cttagccatg cttcggtgat gaaatctttc atagggattg
    43621 ccagttagcc ggccaggcga catacaggaa tttcacgctg gatgtcgtac caaattcgat
    43681 actggagcct ttcgggataa acatcacgtc acccgctttg gcgatcatgg tttcgccttc
    43741 gtggcgaaca tgcagctccc cttccagcac catgtcgatt tcgtcgtagt tcagggtcca
    43801 cgggaagaat gcgttctccc actgcataaa accagcggcc atgctgctgc cgtcgtctcc
    43861 ggtcacaaga tcagtcaagc ccacgcaatg cggctctgcg ccgtcgaaac gaccaaactt
    43921 gacgctgctg ccatcaatca ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg
    43981 ctgcattgcg ccctgctcca gcgactgttt ttccttcatc actttttcca tcagctgtgc
    44041 gaccaggctt tcggtaaact ggccttccgg cagctgggca atgatggttt cgcggatgcg
    44101 ctggctttcg gttttgtcgg ctggcacgct ggcgggcacg gacgccgtta ccgggatcga
    44161 ttcatcacat tcggtgatgg taaaacccag tagatccgcc acttcacggg cttccggggt
    44221 aataatgctg gcgcgcagaa ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg
    44281 aatatcattc gctgtgatga gttttttcac ctgcttcctc ctgtttggtg agagatgcca
    44341 gataatccac cagttgctgc acgctttgcg ggtcgtggct gtttagctcg aaaatcggct
    44401 cttcgaaacc cgtctcaagc aataactttc gcgttgcggc gacgtcagca tctggcatgt
    44461 ccgttttact gatgacggcg atttgccgtt tgctgacgcc aatatccatc aacccggcag
    44521 gcaaccgact ttccgggtca tttgcgccat ggacatagat cagcatgtct acatcctgta
    44581 gcgtggtaat taaggcgtga taccagcggg gatggctaaa atattcaccc ggcgtatcaa
    44641 tatcgccctt atcattaaat tccacggcct gtgtttttct ggcgagggta taattcccct
    44701 gcaacgcatt aaataacgtt gtttttcctg ccccgacggt acccacaaac gcaattcgtt
    44761 tcatgaatgc ctcgtattaa cttttggtca tttcacaaag cgtataattt aataagcgac
    44821 ctaaaccgct gacggtttgt gataacgctt cttctactgc gcccaccgag ccataaatca
    44881 ccaatgcacc gctgaagcgg tcgagaaaac cgatatgcac atcagcggct ttcagcgcta
    44941 aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt cataatgcca attgcgcccg
    45001 catccggaac gccgattttc ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca
    45061 gtgtgacctg tttgccgggc acaaattcct gaatgatgcg ttctttatcc atgagtcgcc
    45121 gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc agagtaaata acaaaattcg
    45181 gcaagcgcgt ttaaaaggtg aggtagatca ctaagaatgg agagatattc gctgtggtgc
    45241 ataaactttc atatgcaacg catgaatata aataaaataa tatcgagata ttatatagac
    45301 tgcttttttt attattcgca aactcagtaa taagctttac taatatattg cagccaaacc
    45361 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa agaattacag cggttgggtt
    45421 tgcgcttcaa ccacggccag cgccaccatg ttgacgatac gacgcaccga tgcgatcggc
    45481 gttaacacgt gaaccggttt cgccacgccc atcagcaccg gaccgacagt cacaccttcc
    45541 gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc
    45601 agaatattgg cagaaccttt caaagggctg tccggcatac ggtcgttgcg aatcgcttcc
    45661 accagcgctg catcgccgtg catttcacca tcaatcatca gatctggcgc acgatccctg
    45721 accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg
    45781 gagtgcgaca acagagcaac gcgcggctca ataccaaaac gacggacagt ttctgccgcc
    45841 atcaaggtga tctccgccag ctcttctgcg tctggttcgt cattgacgta ggtatcggca
    45901 ataaaggtgt taccactcgg cagcagcagc gcgttcattg cacctgcggt gtgaacgcca
    45961 tcgcgataac caaagacatt tttcaccacg ctaaaatgtt catgataatc gcccaccgta
    46021 ccgcagatca ttgcatcggc ttccccacgc tgaaccatga tcgcgccgat cactgtcggg
    46081 ttactgatca gcgcccgctg cgcctgttcc tgagtgacac cgcgacgctt catgatctgg
    46141 aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa
    46201 tcaacgcccg ctttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga
    46261 cgaccgataa ggatcggttt cgccagtccc agcgttacca gttcctgagt ggcatgcaga
    46321 acgcgcgcct cttccccttc tggcagaaca acgcgcttcg gcgctttgcg agcctgggag
    46381 aaaatcggct tcataaacag gttggttttg taaacgaact cagtcagctt gtcgatgtag
    46441 acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact ccatcgcggc tttagcgacc
    46501 gcaggagcga tcttaacgat caagcgcgga tcaaacggtt ttggaatgat gtattccgga
    46561 ccaaagctca gatcctgatc gccatacgct gaagccacca cttcgctctg ttcggcgtgg
    46621 gccagttccg caatcgcgcg taccgccgcc agtttcatct cttcattgat agccgttgcg
    46681 ccaacatcca gcgcgccacg gaagataaac gggaagcaaa ggacgttgtt gacctggttt
    46741 ggatagtcag aacgaccggt gcaaatgatg gcatccggac gcacttcttt cgccagaggc
    46801 ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgcacg agccattttc
    46861 ttcaccattt cctgggtcag cactttcggg ccggaacatc caaggaaaat atccgcgcct
    46921 tcaatcacat catcgagggt acgtttgccg tcatccacca ccgcatatgc ggctttggtt
    46981 tccgccatgt ttggctcacg gccctgatag ataacgcctt ttgaatcgca aaccacgatg
    47041 ttatgttttt gcagacccag cgctaccagc aggttcatac aggcgattgc tgcggcaccc
    47101 gcgccggaaa ccaccatccg cacgtcggag atgtttttct ccaccacgcg caagccgttg
    47161 aggatggcgg cagtgctgat gattgccgtg ccgtgctgat cgtcgtggaa taccggaata
    47221 ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg
    47281 aggttgattc cgccgaaggt cggttcgagc gcagcgacaa cttcaataaa tttgtccggg
    47341 tcgagttcat caacttcaat gtcaaataca tcaatcccgg cgaatttctt aaacagaacg
    47401 cccttgcctt ccatcaccgg tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc
    47461 gccgtaccgt tagagatcac cgccaccagg ttaccgcgcg cggtatattt gtaggctttt
    47521 aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc
    47581 agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga
    47641 aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca
    47701 cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt
    47761 attaccctgc cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg
    47821 ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt
    47881 ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca
    47941 tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt
    48001 tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg
    48061 atgccaccac caatccttcg ctgttactca aagctgccgg attatcacaa tatgagcatt
    48121 taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg
    48181 tcgcagcgtg tgacaaactg gcagtcaatt tcggtgctga aattctcaaa atcgtacccg
    48241 gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga
    48301 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattttga
    48361 tcaaactggc ttcgacctgg gaaggaattc gcgcggccga agagctggaa aaagaaggta
    48421 ttaactgcaa cctgacgctg ctgttttctt ttgcccaggc gcgggcctgt gcagaagcgg
    48481 gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc
    48541 cgatggaccc gtatgtggtg gaagaagatc cgggcgttaa atcggtgcgc aatatctacg
    48601 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
    48661 aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg
    48721 agctgcagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct cagacgttcc
    48781 cacgcccagc tcccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg
    48841 cggtagaaaa actgtctgaa ggcattcgtc tgttcgctgt tgatcaacgc aaactggaag
    48901 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
    48961 gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc
    49021 ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat
    49081 aatcctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
    49141 tcgatgctgc tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg
    49201 aagaacttcc gtcagttgca ttcgaaaacc ccaggccacc cggagattgg ctatacgcca
    49261 ggtgttgaaa ccaccaccgg tccgcttgga caaggtttgg cgaacgccgt cggactggcg
    49321 atagcagagc gtacactggc ggcgcagttt aatcagccgg atcatgaaat tgtcgatcac
    49381 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt
    49441 tcgctggcgg gcacgctggg actgggcaag ctgattggtt tttacgatca caacggtatt
    49501 tccatcgacg gtgaaacaga aggctggttt accgacgata cggcaaaacg ttttgaagcc
    49561 tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg
    49621 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
    49681 ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc tctgggggaa
    49741 gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
    49801 aaagagattt atcacgcctg ggatgcccgc gaaaaaggcg aaaaagcgca gcagagctgg
    49861 aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttacccgt
    49921 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag
    49981 ttgcaggcga atccggcgaa aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct
    50041 tacggaccga tgctaccgga gctgctcgga ggttcagcgg atctggctcc cagcaacctc
    50101 accatctgga aaggctctgt gtcgctgaag gaagatccgg cgggcaacta cattcactac
    50161 ggggtgcgtg aatttggcat gaccgctatc gccaacggca ttgcgcacca cggcggcttt
    50221 gtgccgtata ccgcgacgtt cctgatgttt gttgaatacg cccgtaatgc cgcgcggatg
    50281 gcggcactga tgaaagcacg gcagattatg gtttataccc acgattctat tggcttaggg
    50341 gaagatggtc cgacgcacca ggccgttgag caactggcca gtctgcgctt aacgccgaat
    50401 ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt
    50461 gagcgccaca acgggccgac agcactgatc ctctcaaggc agaatctggc ccaggtggaa
    50521 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc
    50581 ggtaagccag atattattct gattgccacc ggttcagaga tggaaatcac cctgcaagcg
    50641 gcagagaaat tagcgggaga aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat
    50701 attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct
    50761 cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg
    50821 gcaattgtcg ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc
    50881 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt
    50941 gcctgatggt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt cttatccggc
    51001 ctacagcgtc ttatccggcc tacatgtccc cgcaattttg tttaacgggt gatccacaac
    51061 gtgggccagg cgtctggccc atgccagtta tcgcaggtgg gttctgcggc ataacgcacc
    51121 aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg
    51181 cgccctttcg ctaaggtcac cagttcacgc gatttctcat caaatgttgc gttcatcagt
    51241 tccagttcat tcgtctcctg accgttgttg aatggcaaac gcaaccgaac cgggcgcgag
    51301 gctagtggct ttttacgcga cacaatccat gccaaatcaa tggtgttata ggcccctgcc
    51361 tcacagctaa tcatcatcag cgctttatca tcagtcagcg cagtgacatt cacctcacga
    51421 cgtaacggat caagcgagca gcgcagacca ttcatccgcc agtttccata atccagcaaa
    51481 tcgttgcgtt cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc gacctctttc
    51541 agcgcaggcg cgggcggtac gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt
    51601 tcactgccaa cgcgcttttg ctgagcatca ataaacaaca acgccgcttt taagccactc
    51661 agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc
    51721 aaaaacgcgg tgatggttgc cgtatcatcg gttactaata accatggtga aatccgccac
    51781 ttgtcaccac ttaacgccaa cggctcacca tctaacagca gccgtggcgc tatctctcct
    51841 tcagacgcat ccggcgactt caacccgccg cgctcaatac gtaaaacggc atcggtatgc
    51901 gccccggcgc tgcggctcag ggtcatcacc agtccattat gatcgcccgt attgcgcgcc
    51961 acgcagaagt tttggttatt acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt
    52021 gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag
    52081 cgcatggacg gtacgacccc agaagttcaa acaaacagca caagtcgtat cttcgttgcg
    52141 caggtagcgc ggctcaatca gatttgtcgg attgagccaa taataaacaa cgaaatatta
    52201 ttgttatttt tcagtccatt aaatgtgacg tttgcaaata gttaagcaat aacaccgtct
    52261 taccgtcacg tatctcgccg gttttgatca tctccagcgc ctggctgaac ggcagctcga
    52321 gcacttcaat atcttcatct tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt
    52381 attcggcgat aaaaaagtgg attagctcag tcacaccgcc aggcgacata tacagttcaa
    52441 ataatttgcg cacttcgccc acttcatagc ccgtctcttc aatcgcttct ttacgaatgc
    52501 atacttccgg ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt
    52561 cattgccatt aacccaggta gcgacacgga actgacgaat cagaaccacg ctctttttct
    52621 tcgcgttgta caggaggatc gtcgcaccat taccgcgatc gtaaacttca cgtttatggc
    52681 ggataacttc gccatctttg cgcgtgagat cgtaggtaat gttgtgcagg gtgaaatagt
    52741 tatcggagag aattttgtct ttaatgaggg tgatttgttg cgtcataccg actccacagc
    52801 gcgaaatgaa caattatctt acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga
    52861 gatttgactg attttacccc cagccagtca ataatcccct gtgccgcatg acgtccttct
    52921 gccattgcgg taaccaccag atccgcacca cgcacggcgt cgccaccggc gaagattttc
    52981 ggattggtgg tctggtaacg gtactggctt tccacatccg cgatgatgcg gccccatttg
    53041 tctaccgtta caccgtgcga ctccagccac ggcatcccgt gtggattgaa gccaaacgcc
    53101 ataatcaccg catcggctgg catgacaaat tcactgccct caatcggtac aggtcgccga
    53161 cgcccctggg catccggctc gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga
    53221 ccttgttcat tcagctcaag cgccaccggc tggacgttaa attcgaagtt ggccccctct
    53281 tcgcgggcgt tcttcacctc tttcttcgag cctggcatgt tagcttcatc acgacgataa
    53341 gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac gcacacagtc catcgcggtg
    53401 tcgccgccgc ccagtaccac gacgttaagt ccggcggtat tgataaacgg ctcttccggt
    53461 agctcttcga gccccatcac ctgtttagtg ttggcaatga ggaacggcag cgcgtcataa
    53521 acgcccggcg catcttcatt gggtaaaccc gctttcatgg aacggtaagt gcctacgcca
    53581 acgaagaccg cgtcgtattg ttccaaaagc gaatccaaag agacatcttt acccacttca
    53641 caattgagtt cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg tgccagcagg
    53701 gatttatcca gtttgaaaga aggaatgccg aaagtgagca agccaccaat ttctggatgg
    53761 cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa catccgcaca ggccagccct
    53821 gccgggcctg ccccgataat cgccacccgc ttgtccactt tggtgacatg acttaagtca
    53881 ggacgccaac ctttcgccaa cgcctgatct gaaatatagc gttcaatgtt gccgatagtt
    53941 acagcaccgt gctcatcgcg aatagtacag gcaccttcac acaaacggtc ttgcggacaa
    54001 acgcgtccgg taatttccgg cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg
    54061 atgtttccgg ctttcaccag ttcaatccac tgcggaatat ggttatgcag cgggcaggtc
    54121 cattcacaaa cgctgtgctc gccgcactta aggcagcgcg aggcttcccg ttgtgcctgg
    54181 tcggcgcgaa atggcagata aatttcatca aaaccggttt tgcgcgcttc aatcgccagt
    54241 ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt cgactttact cattaccggc
    54301 atttcttgcg ccgcggtact ggcatgccac ggttgatgtt cctgacgcgc ggtgcgcaag
    54361 cggcgggatt tcgccatgcc ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc
    54421 gggcaattct caacacaggc aggaccgttt tcgcgccccg cacaaaggtc gcatttatgc
    54481 gccgtggctt ttacttttcc tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaaa
    54541 ggacaggcca ccacgcagga tttacagcca atacactttt gctgattgac ctgaatgctg
    54601 tcatcaacgt ggctgattgc gccattaggg cagctacggg cgcagggcgc atcttcacaa
    54661 tggtgacagg tcactgcact acgttgctgt tgatgtttga taaccgtaat tcggggatga
    54721 aaatggtgtt ggctcaggac atgttgctca tcattgtgag ccatgacaca ggcgatttca
    54781 caagcatgac aacccagaca ttgctgactg ttggccataa taaaacgatt cataacgacc
    54841 ttcttttttg gttgtaaaaa ccttattctt tatatgagtg ttgttattac ccgacttaca
    54901 ggggaatcgg caatgttcat gtgcccagaa taagtaacta tttcgctata aactgtggca
    54961 gatcaaataa tcccatcact gactaaattg tgtttcagct aactggaaca ttaatgattt
    55021 tttgtggaga agacgcgtgt gattgttaaa cgacccgtct cggccagtct ggcccgggcc
    55081 tttttttaca ttgtgctgct gtcgattctt tccacgggta tcgctctgct aactctggcg
    55141 agcagtttgc gcgacgctga ggctatcaat attgccggat cgctacgtat gcagagttac
    55201 cgcctgggct acgacttgca aagtggcagt ccacaactca atgcacatcg ccagttgttt
    55261 cagcaggcac tgcattcacc ggtattaacc aatctcaacg tctggtatgt gccagaagca
    55321 gtaaaaactc gctatgcgca tctgaatgcc aactggctgg agatgaataa tcggctcagc
    55381 aagggcgatt tgccgtggta tcaggccaat attaataatt atgttaatca gatagacctg
    55441 ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc
    55501 ctggctggcg gtatcggtat tttcacgctg gtctttttta ctttgcgccg tatacgccat
    55561 caggtggttg ccccgctgaa tcagctggtt accgccagtc aacgtattga acacgggcag
    55621 ttcgactcgc cgccgctgga taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc
    55681 tttaaccaga tgtcgagcga gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa
    55741 gaaaagaccc gcgatctcca cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcc
    55801 caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt
    55861 cgcgacaatg aagcggctga atatctggag ttaaatgtcg gtgaaaactg gcggattagc
    55921 gaagggcaac caaacccgga attgccgatg cagattttac cggtgacaat gcaagagacg
    55981 gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac
    56041 agcgtttcgt cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag
    56101 cagttattgt tgatggaaga acgtgcaacc atcgcccgcg aattgcacga ctcgctggct
    56161 caggtacttt cttacttacg tatccagttg acgttactga agcgttcgat accggaagat
    56221 aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga tgcttatcgg
    56281 cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga tctcccctcc
    56341 gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgccaaact gaccctcgac
    56401 tgccgtctgc caaccctggc gctggatgcg caaatgcagg tgcatttgtt gcaaattatt
    56461 cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc
    56521 gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat tggcatcggt
    56581 gaaccgaaag agcccgaagg tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg
    56641 ctgggtggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc
    56701 tttcgctccg cggagggtga ggaaagtcag ctgatgtaat gcctcctact gaccaaagaa
    56761 tacttgcact taaggttcag tataaaaggg catgataatt tacattaact cctttttttc
    56821 tccacgattg gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacgatacgc
    56881 agaaacacga ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg
    56941 ggtgctggca atcctgttgt gtctgacagg taccctggcg attttctcat tgcccgttga
    57001 acaatacccc gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc
    57061 ggcccagacg ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga
    57121 taatctcatg tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag
    57181 ttttaaagca ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc
    57241 agccatgcga aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg
    57301 cgataccaat attctgacca ttgctttcgt ctctaccgat ggttcgatgg ataaacagga
    57361 tattgccgat tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg
    57421 ggatatcgat gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact
    57481 caacagtttc cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca
    57541 gattgcggtt gggcaacttg gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac
    57601 cattaacgcc cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgagggt
    57661 gaatcaagac ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga
    57721 gaaatacgat tatcttagcc gcttcaatgg taagccagcc tccgggctgg gggtaaaact
    57781 ggcctccggc gcaaacgaaa tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct
    57841 ggcgcagtat ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt
    57901 taaagcctcc attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct
    57961 cgttatgtat ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc
    58021 ggtggtgttg atgggaacct tctccgtact ttacgccttc ggttacagcg tcaacacctt
    58081 aaccatgttc gcgatggtgc tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt
    58141 ggaaaacgtc gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa
    58201 atcgatgggg cagatccagg gggcactggt cgggattgcg atggttcttt cggcggtatt
    58261 tgtaccaatg gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac
    58321 cattgttgcg gcgatggtgc tgtcagtact ggtagcgatg atcctcactc cggctctgtg
    58381 tgccacacta cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc
    58441 ctggtttaac cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat
    58501 tctccaccgt agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt
    58561 cctgttcctg cgtttgccga cgtcgttctt accgctggaa gaccgtggca tgtttactac
    58621 ctcggtacaa ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat
    58681 cgagaaatat tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccactgttgg
    58741 ttctggcccc gggggtaacg ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg
    58801 gagcgaacgc gacagtaaga ccggcacctc atttgccatt atcgagcgtg caacgaaggc
    58861 gtttaacaag attaaagaag ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct
    58921 tggtagttct gcaggttttg atatggagtt gcaggaccac gctggagcag gtcacgatgc
    58981 gctgatggca gcacgtaacc agttgctggc gctggcggcg gaaaacccgg agctaacccg
    59041 tgtgcgccat aacggcctcg atgacagtcc gcagttgcag attgatatcg accagcgtaa
    59101 agctcaggcg ctgggcgttg ctatcgacga tattaacgac acactgcaaa ccgcctgggg
    59161 ttcgagctat gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc
    59221 agctgcgccg tatcgcatgc tgccagatga cattaatctc tggtatgtcc gaaataaaga
    59281 tggcggcatg gtgcccttct ctgctttcgc gacctcacgc tgggagacag gctcgccgcg
    59341 tctggaacgc tataacggtt attctgcggt tgagattgtt ggggaagccg caccgggggt
    59401 cagtaccggt acggcgatgg atattatgga atcgttagtg aagcagttgc caaacggctt
    59461 tggtctggag tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc
    59521 gctgtacgcc atttccttgc tggtagtatt cctgtgtctg gcggcattgt atgaaagctg
    59581 gtcggtaccg ttctcggtaa tgctggtcgt gccgctgggg gttatcggcg cgctgctggc
    59641 aacctggatg cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat
    59701 tggtttatcg gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa
    59761 aggccacgac ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct
    59821 gatgacctcg ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg
    59881 ttccggtggt cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagctactat
    59941 tctggctatt tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa
    60001 gccgcgcccg gaataagcaa taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt
    60061 tccgattaag aacctgctca gcgggttctt gctgtttata ctttgtctcg ggaattactt
    60121 acgaagcata acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac
    60181 ctctcttata attatgggta ttctacggaa acaatatacc gtggtgaagc taatttactc
    60241 gattgctgcg atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt
    60301 gataatttgt atgtgataca cagcaacatt tcgagatatt catacggcat ctaatactga
    60361 ttt
//
LOCUS       DAZGCS010000016        59244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGCS010000016 DAZGCS010000000
VERSION     DAZGCS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.016"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..857
                     /locus_tag="ACHK01_001058"
     CDS             9..857
                     /locus_tag="ACHK01_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO8096048.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSVTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQNYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            854..1345
                     /locus_tag="ACHK01_001059"
     CDS             854..1345
                     /locus_tag="ACHK01_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO8096049.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(1387..2337)
                     /gene="corA"
                     /locus_tag="ACHK01_001060"
     CDS             complement(1387..2337)
                     /gene="corA"
                     /locus_tag="ACHK01_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO8096050.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2489..2545)
                     /gene="ysgD"
                     /locus_tag="ACHK01_001061"
     CDS             complement(2489..2545)
                     /gene="ysgD"
                     /locus_tag="ACHK01_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO8096051.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2707..3471
                     /gene="yigE"
                     /locus_tag="ACHK01_001062"
     CDS             2707..3471
                     /gene="yigE"
                     /locus_tag="ACHK01_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO8096052.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3618..5780)
                     /gene="uvrD"
                     /locus_tag="ACHK01_001063"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3618..5780)
                     /gene="uvrD"
                     /locus_tag="ACHK01_001063"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO8096053.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5864..6580)
                     /gene="yigB"
                     /locus_tag="ACHK01_001064"
     CDS             complement(5864..6580)
                     /gene="yigB"
                     /locus_tag="ACHK01_001064"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO8096054.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6580..7476)
                     /gene="xerC"
                     /locus_tag="ACHK01_001065"
     CDS             complement(6580..7476)
                     /gene="xerC"
                     /locus_tag="ACHK01_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO8096055.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7473..8180)
                     /gene="yigA"
                     /locus_tag="ACHK01_001066"
     CDS             complement(7473..8180)
                     /gene="yigA"
                     /locus_tag="ACHK01_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO8096056.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8177..9001)
                     /gene="dapF"
                     /locus_tag="ACHK01_001067"
     CDS             complement(8177..9001)
                     /gene="dapF"
                     /locus_tag="ACHK01_001067"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO8096057.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9038..9241)
                     /gene="yifL"
                     /locus_tag="ACHK01_001068"
     CDS             complement(9038..9241)
                     /gene="yifL"
                     /locus_tag="ACHK01_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8096058.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9591..10145
                     /locus_tag="ACHK01_001069"
     CDS             9591..10145
                     /locus_tag="ACHK01_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO8096059.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10483..11991
                     /locus_tag="ACHK01_001070"
     CDS             10483..11991
                     /locus_tag="ACHK01_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO8096060.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11988..13313
                     /locus_tag="ACHK01_001071"
     CDS             11988..13313
                     /locus_tag="ACHK01_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO8096061.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            13361..13681
                     /gene="cyaY"
                     /locus_tag="ACHK01_001072"
     CDS             13361..13681
                     /gene="cyaY"
                     /locus_tag="ACHK01_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO8096062.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13721..16267)
                     /gene="cyaA"
                     /locus_tag="ACHK01_001073"
     CDS             complement(13721..16267)
                     /gene="cyaA"
                     /locus_tag="ACHK01_001073"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO8096063.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            16654..17595
                     /gene="hemC"
                     /locus_tag="ACHK01_001074"
     CDS             16654..17595
                     /gene="hemC"
                     /locus_tag="ACHK01_001074"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO8096064.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            17592..18332
                     /gene="hemD"
                     /locus_tag="ACHK01_001075"
     CDS             17592..18332
                     /gene="hemD"
                     /locus_tag="ACHK01_001075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO8096065.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            18354..19547
                     /gene="hemX"
                     /locus_tag="ACHK01_001076"
     CDS             18354..19547
                     /gene="hemX"
                     /locus_tag="ACHK01_001076"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO8096066.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFIAIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQGDTPAA
                     APQGE"
     gene            19550..20746
                     /gene="hemY"
                     /locus_tag="ACHK01_001077"
     CDS             19550..20746
                     /gene="hemY"
                     /locus_tag="ACHK01_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO8096067.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            21424..21561
                     /locus_tag="ACHK01_001078"
                     /pseudo
     CDS             21424..21561
                     /locus_tag="ACHK01_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21669..22904)
                     /gene="aslB"
                     /locus_tag="ACHK01_001079"
     CDS             complement(21669..22904)
                     /gene="aslB"
                     /locus_tag="ACHK01_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8096068.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(23051..23127)
                     /locus_tag="ACHK01_001080"
     tRNA            complement(23051..23127)
                     /locus_tag="ACHK01_001080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23091..23093),aa:Pro,seq:tgg)
     gene            complement(23170..23256)
                     /locus_tag="ACHK01_001081"
     tRNA            complement(23170..23256)
                     /locus_tag="ACHK01_001081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23220..23222),aa:Leu,seq:cag)
     gene            complement(23277..23352)
                     /locus_tag="ACHK01_001082"
     tRNA            complement(23277..23352)
                     /locus_tag="ACHK01_001082"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23317..23319),aa:His,seq:gtg)
     gene            complement(23411..23487)
                     /locus_tag="ACHK01_001083"
     tRNA            complement(23411..23487)
                     /locus_tag="ACHK01_001083"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23451..23453),aa:Arg,seq:ccg)
     gene            complement(23590..24975)
                     /gene="yifK"
                     /locus_tag="ACHK01_001084"
     CDS             complement(23590..24975)
                     /gene="yifK"
                     /locus_tag="ACHK01_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8096069.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(25166..25906)
                     /gene="wecG"
                     /locus_tag="ACHK01_001085"
     CDS             complement(25166..25906)
                     /gene="wecG"
                     /locus_tag="ACHK01_001085"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO8096070.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25909..27261)
                     /gene="wzyE"
                     /locus_tag="ACHK01_001086"
     CDS             complement(25909..27261)
                     /gene="wzyE"
                     /locus_tag="ACHK01_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO8096071.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(27258..28337)
                     /gene="rffT"
                     /locus_tag="ACHK01_001087"
                     /gene_synonym="wecF"
     CDS             complement(27258..28337)
                     /gene="rffT"
                     /locus_tag="ACHK01_001087"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO8096072.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFAARGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(28334..29584)
                     /gene="wzxE"
                     /locus_tag="ACHK01_001088"
     CDS             complement(28334..29584)
                     /gene="wzxE"
                     /locus_tag="ACHK01_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO8096073.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29586..30716)
                     /gene="rffA"
                     /locus_tag="ACHK01_001089"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29586..30716)
                     /gene="rffA"
                     /locus_tag="ACHK01_001089"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO8096074.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30721..31395)
                     /gene="rffC"
                     /locus_tag="ACHK01_001090"
                     /gene_synonym="wecD"
     CDS             complement(30721..31395)
                     /gene="rffC"
                     /locus_tag="ACHK01_001090"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO8096075.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(31373..32254)
                     /gene="rfbA"
                     /locus_tag="ACHK01_001091"
     CDS             complement(31373..32254)
                     /gene="rfbA"
                     /locus_tag="ACHK01_001091"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8096076.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(32273..33340)
                     /gene="rffG"
                     /locus_tag="ACHK01_001092"
     CDS             complement(32273..33340)
                     /gene="rffG"
                     /locus_tag="ACHK01_001092"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8096077.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(33340..34602)
                     /gene="wecC"
                     /locus_tag="ACHK01_001093"
     CDS             complement(33340..34602)
                     /gene="wecC"
                     /locus_tag="ACHK01_001093"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO8096078.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34599..35729)
                     /gene="wecB"
                     /locus_tag="ACHK01_001094"
     CDS             complement(34599..35729)
                     /gene="wecB"
                     /locus_tag="ACHK01_001094"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO8096079.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35785..36831)
                     /gene="wzzE"
                     /locus_tag="ACHK01_001095"
     CDS             complement(35785..36831)
                     /gene="wzzE"
                     /locus_tag="ACHK01_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO8096080.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36843..37946)
                     /gene="wecA"
                     /locus_tag="ACHK01_001096"
     CDS             complement(36843..37946)
                     /gene="wecA"
                     /locus_tag="ACHK01_001096"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO8096081.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(38186..39445)
                     /gene="rho"
                     /locus_tag="ACHK01_001097"
     CDS             complement(38186..39445)
                     /gene="rho"
                     /locus_tag="ACHK01_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO8096082.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39530..39631)
                     /gene="rhoL"
                     /locus_tag="ACHK01_001098"
     CDS             complement(39530..39631)
                     /gene="rhoL"
                     /locus_tag="ACHK01_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO8096083.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39772..40101)
                     /gene="trxA"
                     /locus_tag="ACHK01_001099"
     CDS             complement(39772..40101)
                     /gene="trxA"
                     /locus_tag="ACHK01_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO8096084.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            40232..41497
                     /gene="rhlB"
                     /locus_tag="ACHK01_001100"
     CDS             40232..41497
                     /gene="rhlB"
                     /locus_tag="ACHK01_001100"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO8096085.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41631..43115
                     /gene="gppA"
                     /locus_tag="ACHK01_001101"
     CDS             41631..43115
                     /gene="gppA"
                     /locus_tag="ACHK01_001101"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO8096086.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(43162..45183)
                     /gene="rep"
                     /locus_tag="ACHK01_001102"
     CDS             complement(43162..45183)
                     /gene="rep"
                     /locus_tag="ACHK01_001102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO8096087.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALTVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            45400..45849
                     /locus_tag="ACHK01_001103"
     CDS             45400..45849
                     /locus_tag="ACHK01_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO8096088.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            46048..46329
                     /gene="ppiC"
                     /locus_tag="ACHK01_001104"
     CDS             46048..46329
                     /gene="ppiC"
                     /locus_tag="ACHK01_001104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO8096089.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(46416..47891)
                     /gene="ilvC"
                     /locus_tag="ACHK01_001105"
     CDS             complement(46416..47891)
                     /gene="ilvC"
                     /locus_tag="ACHK01_001105"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO8096090.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            48041..48934
                     /gene="ilvY"
                     /locus_tag="ACHK01_001106"
     CDS             48041..48934
                     /gene="ilvY"
                     /locus_tag="ACHK01_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO8096091.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48986..50530)
                     /gene="ilvA"
                     /locus_tag="ACHK01_001107"
     CDS             complement(48986..50530)
                     /gene="ilvA"
                     /locus_tag="ACHK01_001107"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO8096092.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50533..52383)
                     /gene="ilvD"
                     /locus_tag="ACHK01_001108"
     CDS             complement(50533..52383)
                     /gene="ilvD"
                     /locus_tag="ACHK01_001108"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO8096093.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52448..53377)
                     /gene="ilvE"
                     /locus_tag="ACHK01_001109"
     CDS             complement(52448..53377)
                     /gene="ilvE"
                     /locus_tag="ACHK01_001109"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO8096094.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWKRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIDVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(53397..53660)
                     /gene="ilvM"
                     /locus_tag="ACHK01_001110"
     CDS             complement(53397..53660)
                     /gene="ilvM"
                     /locus_tag="ACHK01_001110"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO8096095.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53657..55303)
                     /gene="ilvG"
                     /locus_tag="ACHK01_001111"
     CDS             complement(53657..55303)
                     /gene="ilvG"
                     /locus_tag="ACHK01_001111"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO8096096.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(55306..55356)
                     /gene="ilvX"
                     /locus_tag="ACHK01_001112"
     CDS             complement(55306..55356)
                     /gene="ilvX"
                     /locus_tag="ACHK01_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO8096097.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(55443..55541)
                     /gene="ilvL"
                     /locus_tag="ACHK01_001113"
     CDS             complement(55443..55541)
                     /gene="ilvL"
                     /locus_tag="ACHK01_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO8096098.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            55895..57415
                     /gene="yifB"
                     /locus_tag="ACHK01_001114"
     CDS             55895..57415
                     /gene="yifB"
                     /locus_tag="ACHK01_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO8096099.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(57440..57778)
                     /gene="maoP"
                     /locus_tag="ACHK01_001115"
     CDS             complement(57440..57778)
                     /gene="maoP"
                     /locus_tag="ACHK01_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO8096100.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            57897..58736
                     /gene="hdfR"
                     /locus_tag="ACHK01_001116"
     CDS             57897..58736
                     /gene="hdfR"
                     /locus_tag="ACHK01_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO8096101.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(58832..58907)
                     /locus_tag="ACHK01_001117"
     tRNA            complement(58832..58907)
                     /locus_tag="ACHK01_001117"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58872..58874),aa:Trp,seq:cca)
     gene            complement(58916..58992)
                     /locus_tag="ACHK01_001118"
     tRNA            complement(58916..58992)
                     /locus_tag="ACHK01_001118"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58956..58958),aa:Asp,seq:gtc)
     gene            complement(59047..59162)
                     /gene="rrf"
                     /locus_tag="ACHK01_001119"
     rRNA            complement(59047..59162)
                     /gene="rrf"
                     /locus_tag="ACHK01_001119"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        14676 a        15788 c        14909 g        13871 t
ORIGIN      
        1 aaatgcaaat gaataacata ttacttccgc caattaatat tccctgtaca cttttcgaaa
       61 ctattagttt attcgatgat ttttctgctg atgatatgca atatggtgat atggtagaac
      121 aagactttct ctcactcggc ctaagcgata tctcagcaaa agttgatcct tacagactta
      181 ttaaatacca tttccctggc ccaggttcta ttaatgttgc ttttagtgcc tcaagttccg
      241 taacaaaaat atcacaacgt gaatgcactg atattttatt tgcagaaatg aaagaactag
      301 caaaaatgtt ttcctttttt ggacaaaata aaacacttat cgaagatctg attgaacatt
      361 ttagatatgg aaatggcagc aattttcata gccaacaatt aaatttatct tttcatgaaa
      421 aaataaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      481 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      541 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      601 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaactacg
      661 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      721 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      781 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      841 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      901 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      961 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
     1021 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
     1081 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
     1141 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
     1201 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1261 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1321 tgactttaaa aaagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1381 ctcatattac aaccagttct tccgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1441 cataaagata atcgcgcctg ggtagccgaa actccacttc aactctggca taaattcaaa
     1501 gttcatcccg tagctggaag ccaccagcgt cggtggcagg aacaccacgg ataccaccga
     1561 gaagattttg atgatgcggt tctgctcgat gttgataaag cccatcgccg cctgcatcag
     1621 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1681 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gccttgcgta ccaggaagtt
     1741 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1801 cgccagcgtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1861 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1921 ggtttcgaac agatccagca gcaactcgta ggcgttaccg tctaccatcg actggctacg
     1981 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     2041 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     2101 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     2161 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     2221 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2281 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2341 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2401 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2461 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2521 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2581 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2641 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2701 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2761 atttttctca ccctcacctt acttcccttg tttgctgttg ccgctgatga ttgcgcactc
     2821 tcagatccga cgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2881 atgtactggc aaaaagctaa tggcgaagcg tggggaacgt tacatgctct gctggcggat
     2941 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     3001 gcgccgctcg gtttgtacat cgaaaacggt cagcagaagg tggcgttaaa tctcgcttca
     3061 ggtgaaggga atttctttat ccgtcctggc ggcgtgtttt atgtcgcggg agataaagtc
     3121 ggcatcgttc gtctggatgc cttcaaaacc agtaaagaga ttcagtttgc ggtgcagtca
     3181 gggccaatgt tgatggaaaa cggtgtaatt aatccgcgta ttcatcccaa cgtcgcctca
     3241 cgcaaaattc gtaacggtgt tgggattaat aaacatggga atgccgtgtt tttgttgagc
     3301 cagcaggcga caaattttta tgattttgcc tgttatgcca aagcgaaact gaacgttgag
     3361 cagctgcttt atcttgatgg cacgatttca catatgtata tgaaaggcgg ggcaatcccg
     3421 tggcaacgct atccttttgt caccatgatt tccgtcgagc ggaaaggtta gaatgatagt
     3481 tttgtgtcag atactaaata tgctgctgat gccggatgcg gcgtaaacgc cttatccggc
     3541 ctacatgacg ttgcaattta ttgaatctga atgatttttt aggccaaata aggtgcgcag
     3601 caccgcatcc ggcaacgtta caccgactcc agccgggcat aagccgccac cagccattta
     3661 ataccctggc cctgaaatgc cacctgcaaa cggctatgct caccgctgcc ttccatattg
     3721 acaatggtgc cttcaccaaa cttagcgtgg cgtacgcgct ggccgagctt gtagccgctg
     3781 tcgttctcga ccatcggcgt acccatccgc tgatggctga ccgggcggct taccgtggcg
     3841 cgcaggcgca cctcttccac acactcttcc ggcagctcgc caataaagcg cgacgggcga
     3901 tggtaaacct ctttgccata cagacggcga gtttccgcgt aggtcagcgt cagtttctgc
     3961 atcgcgcggg ttacgccaac gtaggccaga cggcgttctt cttccagacg cccgccttca
     4021 tccagcgaca tctggcttgg gaacataccc tcttccatac cgacaataaa cacctgcggg
     4081 aactccaggc ctttcgccga gtgcagcgtc atcaactgca ctgcatcctg ccaggtatcc
     4141 gcctgcccct cgcccgcttc cagcgccgca tgggagagga atgcctgcag cggcattaaa
     4201 tcttcatctt cttcgttgta gctgaactgg cgcgttgccg tcaccagttc ctctaagttt
     4261 tcgatacgcg tctggccttt ttcgcccttc tcctgctcgt acatggtgcg caggccggag
     4321 tctttaatta cccggtcagt ctgtacatgc agcggcatat cggcagtttc ttgcgctaag
     4381 gcatcgatta attccataaa tcgctgcaag gcgctggcag ctcgcccggc gagggctttt
     4441 tcctgcaaca gttcacgaca tgcctgccag agtgttaact ggcgatcgcg cgatgtctga
     4501 cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg gcgtattcac cacgcgctca
     4561 aacgccgcgt cgtcgttacg gttggcaatc agacgcagat acgagagcgc gtctttgatt
     4621 tcctggcgtt cgaagaagcg catcccgccg taaatacggt acggcatact ggcctgcaac
     4681 agtgcttctt ccagtacacg cgactgggcg ttactgcggt agagaatggc gcactcggca
     4741 agcgccccgc cgttgtcctg ccaggttttg atgcggttaa ccacaaaacg cgcttcatcg
     4801 agttcgttaa aagcgcaata gagggaaata ggctcaccgt ccgcgccatc ggtccacagt
     4861 tttttaccca gacgcccgtt attgttttca atcagggcgt tagcggcgct cagaatattg
     4921 ctggtagagc ggtagttttg ctccagacga atagtttcgg caccggggaa atcattaagg
     4981 aaacgctgaa tattctccac ctgcgccccg cgccagccgt agattgactg gtcgtcatca
     5041 ccgacgatca tcactttgcc ggtgtcgccc gccagcaggc ggatccacgc gtactgaatg
     5101 ttgttggtat cctggaattc gtccaccagg atattggtaa aacgttcgcg gtagtgttgt
     5161 aggatatgcg gcttgttaag ccacagttcg tgagcgcgca gcagcagctc ggcgaagtcc
     5221 accaggcccg cgcggtcaca cgcttcctga tacgcctgat acaccttctg ccaggtctgc
     5281 tccaccggat taccgtagct ttgaatatga tgcggacgca ggccttcatc tttctggctg
     5341 ttgatgtacc acattgcctg gcgcggcggc cactgcttct cgtcgaggtt catggctttg
     5401 atcagacgct taagcaggcg cagctggtcc tcgctatcga ggatctggaa atcctgcggc
     5461 agattggcgt ccatatggtg cgcacgcagc aggcggtgcg ccagcccgtg gaaggtgccg
     5521 acccacatgc cgccctggct ggtgcccatc agttgcccga tacgatgacg catctccgcc
     5581 gccgctttgt tggtaaacgt caccgccata atcgagtatg gcgagcagtt ttccacgctc
     5641 atcagccagg cgatacgatg caccagtacg cgcgtcttac cactgcccgc gcccgccagc
     5701 accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct gtttgtcatt aaggctgtcg
     5761 agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat aggttgggcg gagaagcgca
     5821 acgccctccg ccaaaaagct gggtatatat acagatttgc tgattatatc agcgaggtca
     5881 gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctgtcccag gtttgcatca
     5941 gatcgccatt ttccggtctg atccaacaag cctgcattcc gctgcgaatt gccccaccca
     6001 cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg ttgagttttt
     6061 ccgcagccaa aaagtacata tcgctgaacg gttttgagcg cccgtgcggg ccagcgcgca
     6121 gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgggcgtta ccgttggtga
     6181 tcgccaccag cggccatttc ttcgccagct gttttaaggt gtcgtgagtt tgctgcggga
     6241 cgtcgattcg gctgcgccat ttggcaaagt taatcattgc tgcgtgtgcg cctgcactgg
     6301 cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga aaacgccaac
     6361 gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc aggcgttgca
     6421 gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag gtaagcgcct
     6481 ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc aggtcaaagg
     6541 tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat tatttccccc gtttggcgcg
     6601 tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa tcaagatgag tatagatttg
     6661 cgtggtggag aggttggcat gacccagcag ctcctgcaca ccacgaagat cgccgctcga
     6721 ctccagcata tgcgtagcga acgagtgacg taatttatgt gggtgaacgt gattattcag
     6781 cccttgtttt atgccccatt cggcaaagcg tttctgcaca ttacgcgcgg agatacgctt
     6841 gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca aacaggtcgc gcaaatcaag
     6901 ccagtgctca atccacgcca cagcgttgcg accaatcggc aggcggcgct ctttgctgcc
     6961 tttccccatt acccacactt caccagactc caggtcgagg tgtttgatat ccagccccac
     7021 cagctcggaa agacgcagac ccgcgccgta catcacttcc agcattgcac ggtcgcgtac
     7081 agcgagggga tcgttgatat caatatccag cagccgattc atatcgtcga cgtcgatgtt
     7141 tttcggcaga tgacgcggcg ctttcggtgc cgaaactcct ttcgccgggt tagctttgag
     7201 ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc gcagaaagcc gtaacgccag
     7261 acttgctgct cccagccctt tacggcgact gcgtacagca aaattgcgca ccatcgtcac
     7321 atcacattgc tgccagcttt gcaggccgtt ttcgctggca aaattgatga tcgcctcaag
     7381 ctgacgctgg tagttaagca gggttatcgg gctaagctgg cgctccacgc tcagataacg
     7441 taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc gttcaatcca acgctccaga
     7501 agctccggca acatcagcgc aatttcatga agtaactgcg ttccttgccc ttgttgatag
     7561 tgactggcat cgcgactggt aaacagcaca acacccaaat cagcatcgct tcccagcatc
     7621 gacatcgcca ccgatcccac cgctttcgct tccggtagca ccaccagcaa ctctggtccg
     7681 ttaagcggcc caagatagtg ctgttcctgc cccaaacgct gaatacgcag cggttcgaaa
     7741 gactgacggc ttaatgccag atgagtgtgg ttcgacggcg cacctaagcg ccagcgatcc
     7801 ggaaacaggc gcagactcgc acctgccagg ccgagatcgc gcgcccagcg gtgaaagcgc
     7861 atcagcatat cgtcgagact gctggcggcg gtgagactgc gctgcaggta gagtaggcga
     7921 taaaacaggc cttcgttggc gatagcctgt tccatcaaca gcgccatgtt ctcttccaga
     7981 acatgaatat gattacgtgc gcgggccatg tgccactcga ccaacgaaac ggtgccgcgt
     8041 accggatgcg gcacacgtat cgcttctact gcgcgcgcat tacggataaa aaactcagga
     8101 tttttaatca gataatcgac aaccgcccgg tcatcaagct ccgtgagtgt ttcctgcagt
     8161 tcttcccctg gttgcttcat agatgaataa atccgtcgta gacatgtacc gccgggccag
     8221 tcatatataa cgggtgaccc ggacctttcc aggcgatatc aagacgaccg ccggggagtt
     8281 ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc taccgccacc gccgcacatg
     8341 cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc ataaacgcgt aaacgaatat
     8401 gctcgcgctt aaccacttgc ataaaaccga tattggctcg ctccggaaaa cgctcgtggc
     8461 tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg acctgaatca
     8521 cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg gcggcgcgca
     8581 taatataggt cttttccgct ttgttagcgc gaaacggcac ggcggaaggt tcgaagttgg
     8641 gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc atccgcccgt
     8701 tggcggtgct gacgcggata tcgcgcttat tggtcagccc tttcagacgc acaaaacggg
     8761 caaagcagcg cgcaccattg ccgcactgcg ccacttcact gccatcagca ttgaaaatgc
     8821 gatagtgaaa atccagttca ggatcatacg gcggctcaac caccagcagt tggtcaaacc
     8881 ctactcccag gtgccgatca gccaggcgac gaatcagctc cggtgaaaaa aagacattct
     8941 gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc gagaactgca
     9001 tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc tgggatggac
     9061 catcgccagt ggcgcgatcg tttttatccg gcaccgtgga ttgcgtttgc gtctctaccg
     9121 gtttggtcgg cggcggtgcg tttttatctg caggcgggaa atagagcgga cctttcagac
     9181 cgcagcccgt caggctgaag agagtaatta atacagtgag tgccttaaac acgtttttca
     9241 ttatggattg cctgtaaagt tcatcgcttt ctgctttcta tcatcgcagg agaaggcgta
     9301 aaagcaagcg tttagcgact ctttatgaag tctgaaaagt gaggaaatgc tgcgagtata
     9361 acgcttacac gtggggattt atgttttgct ggtagtttcg tgatcggcac ctcgcgccag
     9421 gatggcttta tgcactatcg caatgtggtt tgtttgttgt cttgttcatt gttcttatcg
     9481 tcggcatggg gcttttaata gagtggcgca ccatgcaatt tcgttgtctg accttagtcg
     9541 ccagtgacat acaactttgc caacctggtg ataagaggga ataaaatctg atggggctat
     9601 ttacccaact ggaaaaattt gaccaaaagc ttacgcgcgg ttatgcccgg tgggggcgct
     9661 gggtatggcg gacgttaatt gtcgttccgg tactggtcgt gttgtggaat ataggacagg
     9721 cagtatgggg aggaccaaga ggtggagtga ttctggaaat ccacagtgaa attgaccgcc
     9781 cgatactggg attcagtgtt aacggcgtgg cgggcgcgaa tgcttttgca aatggtggcg
     9841 gttcaacaac ctgttgtggt gatgttagtg gggatacggc agaggttatc tggacattaa
     9901 gtactactcg tacacagtat aatgctggaa tgcgtctgga gaaacggaat atgacgttac
     9961 ctctccccaa aagagaatgg ggagaagatt ttctgcatgt gcattttatg cctggcgata
    10021 aagtgctgct ggggtggagt aaagattctt tttctccgta tgaagatctt cacaacgggg
    10081 ggtacaagac ccgcgttcgg caggatgtga aagataaact ttatggtaca gggaaaatga
    10141 attgatagat ttctataaaa atcacgttcg gcagaaagcg taataatgtg gggaatgtta
    10201 gataccggtg gtgggaccta agtacaacac gcgaaagaca ataaagtagg taacggtggg
    10261 cagagtcgca tgttgacacc cttcccggcg tcaatccggc acattttaac gtgatcagtc
    10321 acaccatggc gcagtagctc gtatttatat cgtgaatgaa aacctcaagt ttaccccagc
    10381 aactatcaga aaatagcgat ggcctttata gattctgaaa agtgacgatt tgccgcaaaa
    10441 gttacgctta cgtgcgagga attatgttct gctggtagtt tcgtgatcgg cacctcgcgc
    10501 caggatggct ttatgcacta tcgcaatgtg gtttgtttgt tgtcttgttc attgttttta
    10561 tcgtcggcat ggggatgtcg gcttgatccg cctgaacata atatttacca gaaacaggga
    10621 aaaggcgtgg tatacctgcg cccttataaa gaaacaaatc tttctcttcc tgacgtgaat
    10681 tacaaaagtt tgcgccgttt acccaattta ttaatagacc cgacaacact agacaaatgg
    10741 gataaagtac ctcctctgac tgatctgaca accgactacc tctatgaagg cgcacaggcc
    10801 tggtatcctc attactcctg gcatagcgat ggtcgctata tcctctacgc gggtgaagtt
    10861 gtgcaaaacc cgccagacaa acctcccgtc gatgtcgcct cgttcaaggc gtggggtgat
    10921 tttgccgcag ataaacacag tctctatttc gaaggcaagc gcaccgataa caatggcggg
    10981 ggaaatcgct tagatatcaa aacactgcat caggtggaat tccggcctcc ctgggatcct
    11041 gacttactgg ggctgatatt acgtgacgct aatttcttgt atatcaatgg gcatcgcctc
    11101 gcagatcccg atagtttccg tgtgctggca caaaagtcat gggatcagcg aggaaagttt
    11161 tctaccgcgt ttaatccctg tgtggctatg ccatttggcc cctgggatac cctggctcgt
    11221 acacggacca aaatcctgat caacggcgaa cagcttgatg ccgacccgga caccttttcc
    11281 gtcgtgcgct ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg gctgcagcgt
    11341 aaagtcctcg acaaggaaaa tctggagtgg gatgaagatt taacaaagca ctgtctggat
    11401 ttttctctgc tggaaaagaa agtgttctgg cgtaaaggac ctgcttgtaa acaggaagaa
    11461 ttacccggac tcgatccgga gcagtttcaa cccatcagtg atgctgtcgc ccagtatcag
    11521 gactcgcttt ataccatcat cgaaacagag tctggtgacc gcaagctgga aatcgtgaaa
    11581 cttgatgatc ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg ccacggctat
    11641 ttacttacgc gtgccgaagg gtggccatac cattccggtt tacatgtgtt tgaatctgac
    11701 ggaccgctga tcttactgga taaccgttct ccggatgaac gcgaagccca tcttaatgac
    11761 catccctttt tgcgcagatg gtatgcccgc gataaccgct acatttacag ctttgatggc
    11821 gcgcagctct ggcgataccg caccgctgat ccgaaacaag ttcgcttaat ctggaaggaa
    11881 caacattcgg gatatggcta tggcgtaaat tacaaaacgg gatatctgga cggaaaaatt
    11941 actgatgacg gcgaatttat tcctgccccg cgcaatgagg cgacaaaatg aagggtcaaa
    12001 taatcacagt tgcactcatc ctgttgggac acacatttct gacgcctgct gtacaggcca
    12061 tagggttcga ctattacaat gatcacggtg ttatgtctta cggtaaaggt tattgggggg
    12121 ataagaaaat cattgcccaa tttccgaaga tgaacagagc cgatttgcgc cttgtgacaa
    12181 atatatccgg ggagcgggag ttggtggaag gctatatacc caccgatgaa ataagcataa
    12241 aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg
    12301 ttagcaatcc accgggaaca cccactgtcg atattgccag ctttcaggct atgggccgct
    12361 tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt aatagcggcg
    12421 cgtcacgtgt tgatctggcg acactaaaag ctatcgaagg taactctacc acgctggtgg
    12481 atagtaaaaa tctctatttg tccggtcgac gtcaggggag cagcagtaat gttactgtat
    12541 tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga aatttgtatt
    12601 ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt caccttacgg
    12661 cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg gtttttcgcg
    12721 acaataaggg tatgcatcgt tatccctttg ggcaattgtc tggcaaagcg atacccgtag
    12781 atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgt
    12841 ccgaccgtca gtggagcaag tggatagacc taccagatat tgaacctgaa caattccatc
    12901 tgattactgg caatattgcg caatataaag atcggttgta tgtaacaaaa ttatcgacat
    12961 ttggtgaaga ccagcttgag ataatcccgc tggatacgcc agacctggtc attgatcgct
    13021 catttaatag cggcaaacag catgcttatt ttatccgcca attacggtca aagagcgtgc
    13081 aaattattcc agttaacggt ccgctaacta aaaacgatcg cttcgcttat gacgatcgca
    13141 atgtttatac atggaccgat acagaggtaa ggattacgcc ctccccctgc ccggcgaaaa
    13201 cacacgtcag agaagagaat gtgcgtgaac ttcacaacag agacattatt attccgttga
    13261 cggatgactc atgccggaac gcagcaacag acgggcaaac tttgaagccc tgaccccata
    13321 ccgctatact gccgccatca cttaaagaca ggatacaaca atgaacgaca gtgaatttca
    13381 tcgcctggct gatcaactct ggctgaccat tgaagaacgc ctggacgact gggatggcga
    13441 cagcgatatc gactgcgaaa tcaacggcgg cgtactgacc attacctttg aaaacggcag
    13501 caaaatcatt attaaccgcc aggagccgct gcaccaggtg tggctggcaa ccaaacaggg
    13561 cggctaccat tttgacctaa aaggcgatga atggatttgc gatcgcagcg gcgaaacctt
    13621 ctgggatttg ctggaacagg cggcgacaca gcaagcgggt gaaacagtca gtttccgcta
    13681 agattgcatg ccggataagc ctcgctttcc ggcacgttca tcacgaaaaa tattgctgta
    13741 atagcggcgt atcgtgatcc tgattggcag gcggcatgtt accgatagat tttgtgcgga
    13801 acggaatcac ctgttcacga ccatcaacct tcacaatctg atagaactgc ggcaggttga
    13861 agttgatgaa gcttgagccg taggtgaagc ggtcgtgcga tgacgagtag aagcgactga
    13921 cgtcacgcac cagttcctct ttgctgcctt cgcagtggtg atacacctca acccggttgc
    13981 tttcgtcgag aatgtagata ttaaagccat tctcgtcttg cgtttcttcg aagaagaact
    14041 ggatgatccc ttcactagca aagccgtcca ccaccgccgg taatttgacg tgattggttt
    14101 caacctgcac tgacaagccg tgcagtttgt tatgcgaaat agcgccataa aactcgatgg
    14161 cgttttccag tttttgtacc gatacattca ggcgttcgaa gaacaacccc caggtttgac
    14221 cagaaacgcg cagcgccttg aaacgcccgg tttcctggcg ggtgctggaa aggcgcagtt
    14281 caatacactc agaaaccagt tgctgcacgc gagtacggat taacccgcgc agatgctggc
    14341 tataacagaa gacttccacg ctatctggcg gtgcggcgtc ctgatgcatt ttgccgagaa
    14401 tagttttcag ggcttcgatc atcgattgct cgccgttgaa gtgcagcgta cgcacttcgt
    14461 tccacgagtt gcggtacagc aggtcaacgc tacctaccag gcaattttga ttctcgccaa
    14521 agctgaagac atccagctta cggaaatcaa aatgcaccac ctgattgcgg aacgccgctg
    14581 tcgggtcata ttccaggtta acgataatcg ccagatggcg gatctcacac gggctgtaga
    14641 gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg gtgcgacacg tcggcgacca
    14701 tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt aatatacaaa cgggtgcgcg
    14761 aggtcagcag gccgttaaac catgcccacg ccaccagttt gttcaggtaa cggttatatt
    14821 ccagcggctg atggctgatg atcgactcaa tatttggcgc gcggttatac agataccaac
    14881 ctgaacggtt agcacggccc ggcggcacat aaataaaggt cagattcggt tccgagagat
    14941 cgggtgaaat ctgcgggttt accagcgtca ctttacctgg taatgcttca aacgcggcat
    15001 acagcttacg cgtcagcacg ccgatatcct gcggactggc ggagacgcta aggttattgc
    15061 gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat cgcgtcgagc aactcgttgt
    15121 gcgcctcacg cacctgatca atcttccagt tagcgcggtt atcgagcatt gccagacgag
    15181 cttcgtccca accccactcg ctcactaact ggctcaacac tgcgcgacgc cagcctacgc
    15241 aggcgcgttc acggctgagc ttttcgcaca cttttaaata gaagcagcga cgtactaaat
    15301 ccagacgggt aaaatcttca atcgccgtca ggtattcagt aacacgctcc agcatcatgc
    15361 agtatggatc gagaccaaac gatacaatct cgccgtcgtg caaacgctgt ttgatatctt
    15421 tcgccagcag acgtgggttc gggtattccc aggaataggc ttccagcagc agtgttttca
    15481 gtaccgcttt gtatggggaa tcgatactct tgtagagctg ccaaaggctg gcaccaaagt
    15541 actcttcagc agaaagcgag cttaagccac cgagatccag ccattcattt ggcgtcagca
    15601 cgccctgcgc gtaaagcgtc atcacatagt cgtcgtaatg ctcttcttcg tcgcacggca
    15661 ccatattcca cagaatacgc ttaccggcga gacgcacggc ggtacgataa aattcgtcaa
    15721 gcagcagtat atgctgggtg gagccacaat cttcgccccc caggctgccg ctttcattat
    15781 gacggaagcg gttttcatca atcaggaaga agctgacttc cacacccagc gaggcggccc
    15841 agctttccag cagactacat ttacgttgta gtaactggcg ctcttcgcta tcgagccagg
    15901 attgatgaca gacccagata tccaggtcag aggaacaact ttgccctacg gacgaggtgc
    15961 tgcccatggt gtatacacca gtaattggaa gctcaccttt cggtgggtcc tgtactgaca
    16021 ttccacgata cagttcaagc tcgttcaggt agtggcgttg agtttcatca ggcgtgtaaa
    16081 ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg cattagcgga tggtgatagt
    16141 gcaacaatgt cggcagtaga ctgtagacct gttggaatgc aggccccata gcagcaagcg
    16201 cgcgatccac acgcaattga tttatggcat ccagtctctg tttcagagtc tcaatataga
    16261 ggtacaagac gtatcgcctg atttgctacc cgtcatgact gtgattccgc caacatcaac
    16321 ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt aaccgttacc acttttcgcg
    16381 cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa cgtgatcaat ttaacacctt
    16441 gctgattgac cgtaaagaaa gatgcgctac atacaagtgt agcaccgttt attctctgta
    16501 aattccttat tacgacggcg tgaaacgcct gtcaggatcc actgccagac ctcattttac
    16561 ggtttgcgca ggcgtctacg tttcaccaca acactgacat cactctggca aggatgttag
    16621 gatggaccac ggatgataat gacggtaaca agcatgttag acaatgtttt aagaattgcc
    16681 acacgccaaa gcccacttgc actctggcag gcacactatg tcaaagacaa gttgatggcg
    16741 agccatccgg gcctggtggt tgaactggta ccgatggtga cgcgcggcga tgtgattctt
    16801 gatacgccgc tggcgaaagt aggcggaaaa ggcttatttg taaaagagct ggaagtcgcg
    16861 ctcctggaga atcgcgccga tatcgccgta cattcaatga aagatgtgcc ggttgaattc
    16921 ccgcaaggtc tggggttggt cactatttgt gagcgtgaag atcctcgcga tgcctttgtg
    16981 tccaataatt atgacagtct ggatgcgtta ccggcaggca gtatcgtcgg gacgtccagt
    17041 ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc tgattatccg ctccctgcgc
    17101 ggcaacgtcg gcactcgcct gagcaaacta gataacggcg aatacgatgc catcattctt
    17161 gccgtagccg gactaaaacg tttaggtctg gagtcccgca ttcgcgccgc gttaccaccc
    17221 gagatttctc ttccggcggt aggacaaggt gcagtcggta ttgaatgccg ccttgatgat
    17281 acgcgcactc gcgagctgct tgccgcgctg aatcaccacg aaactgcact gcgcgttacc
    17341 gcagaacgcg ccatgaatac ccgtctcgaa ggtggatgtc aggtgccaat tggtagctac
    17401 gccgagctta ttgatggtga aatctggctg cgtgcgctgg tcggcgcgcc ggacggttcg
    17461 cagattattc gcggtgaacg ccgcggtgcg ccgcaaaatg ccgaacaaat ggggatttcg
    17521 ctggcagaag agctactgaa taacggcgcg cgcgagatcc tcgctgaagt ctataacgga
    17581 gacgccccgg catgagtatc ctggtcaccc gcccgtctcc cgctggagaa gagttagtga
    17641 gccgtctgcg cacactgggg caggtggcct ggcattttcc gctgattgag ttttctccgg
    17701 gtcgacaatt accgcaactt gctgatcaac tggcggcgct gggggagagc gatctgttgt
    17761 ttgccctctc gcaacacgcg gttgcttttg cccaatcaca gctgcatcag caagatcgta
    17821 aatggccccg actacctgat tatttcgcca ttggacgcac caccgcactg gcgctacata
    17881 ccgtaagcgg acagaagatt ctctacccgc aggatcggga aatcagcgaa gtcttgctac
    17941 aattacctga attacaaaat attgcgggca aacgtgcgct gatattacgt ggcaatggcg
    18001 gtcgtgagct aattggggat accctgacag cgcgcggtgc tgaggtcact ttttgtgaat
    18061 gttatcaacg atgcgcaatc cattacgatg gtgcagaaga agcgatgcgc tggcaatccc
    18121 gcgaggtgac gatggtcgtt gttaccagcg gtgaaatgtt gcagcaactc tggtcgctga
    18181 tcccacaatg gtatcgtgag cactggttac tacactgtcg actattggtc gtcagtgagc
    18241 gtttggcgaa actcgcccgg gaactgggct ggcaagacat taaggtcgcc gataacgctg
    18301 acaacgatgc gcttttacgg gcattacaat aactctcata acaggaagcc ataatgacgg
    18361 aacaagaaaa aacctccgcc gtggttgaag agaccaggga ggccgtggac accacgtcac
    18421 aacctgtcgc aacagaaaaa aagagtaaga acaatactgc attgattctc agcgcggtgg
    18481 ctatcgctat tgctctggcg gcgggtgtcg gtttgtatgg ctggggtaaa caacaggccg
    18541 tcaatcagac cgccaccagc gatgccctgg ctaaccaact gacggcattg caaaaagccc
    18601 aggagagcca aaaagccgag ctggaaggca ttattaagca acaagctgca cagcttaagc
    18661 aggcgaatcg tcagcaagaa acgctggcaa aacagttgga tgaagtccaa caaaaggtcg
    18721 ccaccatttc cggcagcgat gctaaaacct ggctgctggc tcaggccgat tttctggtga
    18781 aactcgccgg acggaagctg tggagcgatc aggacgtcac gaccgctgca gcgttgctga
    18841 aaagtgcaga tgccagcctg gcggatatga atgacccgag tctgattacc gttcgtcggg
    18901 caattaccga tgatatcgcc agcctttctg cagtttcgca ggtggattat gacggcatca
    18961 tccttaagct taatcagctt tcaaatcagg tagataacct gcgtctggcc gataatgaca
    19021 gcgatggttc gccgatggat tccgacggtg aagagctttc cagttccatc agcgaatggc
    19081 gtatcaatct gcaaaaaagc tggcagaact ttatggacaa cttcattgcg attcgccgtc
    19141 gtgatgacac cgccgtaccg ctgttagcgc caaatcagga tatctatctg cgcgaaaata
    19201 ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg tcaccaggaa gagacttatc
    19261 gccaggcgct ggagaacgtc tccacctggg tacgagctta ctacgatact gatgatgcca
    19321 ccaccaaagc gttcctcgac gaggtggacc agttaagcca gcaaaatatc tcgatggatc
    19381 ttccggaaac cctgcaaagc caggcgatgc tggaaaaact gatgcagacc cgcgtgcgta
    19441 acctgctggc acaaccggca gcggggacaa cggaagctaa acctgcacct gcacctgcac
    19501 ctgcaccgca aggtgatact ccggcagccg cgccgcaagg agaataacca tgctaaaagt
    19561 gttattactc tttgttttgc tgattgcggg gatagtggtt ggcccgatga ttgccggtca
    19621 tcagggttac gtgctgatcc agactgacaa ctacaatatc gaaaccagcg tcacgggcct
    19681 ggcgattatt ttgattctgg cgatggtagt gctgtttgcc attgagtggc tactgcggcg
    19741 gatcttccgc acaggcgcac acacccgtgg gtggtttgtc ggacgtaagc gtcgccgtgc
    19801 ccgtaagcag accgaacagg cgctgctgaa actggcggaa ggcgattatc agcaagttga
    19861 aaagctgatg gcgaaaaatg ccgatcacgc ggaacaaccg gtggtgaact atctactggc
    19921 tgccgaagcc gcgcaacaac gtggtgatga agcacgcgcc aaccaacatc tggaacgcgc
    19981 agcagagctg gccggcaatg acaccattcc ggtagaaatc acccgtgtac gtctgcaact
    20041 ggcccgtaat gaaaaccatg ctgcacgcca cggcgtggat aagctgctgg aagttacgcc
    20101 acgccatccg gaagtattac gtctggcgga acaggcgtat atccgcacag gtgcatggag
    20161 ttctctgctg gatatcatcc catcaatggc gaaagcccat gttggtgatg aagaacatcg
    20221 tgcaatgctg gaacaacagg catggattgg cctgatggat caggcgcgtg ccgataacgg
    20281 tagtgaaggt ttgcgtaact ggtggaaaaa ccaaagccgg aaaacgcgtc atcaggtagc
    20341 gttgcaggtg gcaatggcgg aacatcttat tgaatgtgac gatcatgata ctgcccagca
    20401 aattatcatc gatggcctga aacgccagta cgacgatcgc ctactgctgc cgattcctcg
    20461 actgaaaacc aacaatccgg aacagctgga aaaagtgctg cgccagcaga tcaaaaacgt
    20521 cggcgatcgc ccgctgttgt ggagcacact gggccagtca ctgatgaagc acggagaatg
    20581 gcaggaagca tcgctcgcct tccgcgcggc gctgaaacaa cgtccggacg cctacgatta
    20641 cgcatggctt gccgacgcgc tggacagact gcacaagccg gaagaagctg cagcgatgcg
    20701 tcgcgacggt ttgatgttaa cgttgcagaa caacccacca cagtagttcc ttctcacccg
    20761 gaggcaagca cctccgggac ctttctgatg cataaaaaaa cgcctgctct tattacggag
    20821 caggcgttaa aacaggtctg tatgacaaca agtgggtgct tcactcaacg ttgtgtccat
    20881 ggtgtctgat gaggcataag cgacatctgt cagtggacga taagcaccgt aaacggctct
    20941 gcgtcattcc ggagtttatg aggcactaag gcgaacataa gagatggaat gagcatctac
    21001 ccgtttatta tgccacagag aatcgggaaa taacatccct taacacttgt tatgagataa
    21061 ttctgtaatc ctctttgctt cctgagtaat aacttcctga gtgaatattt aacctgagct
    21121 tgatcctaca catacttatt atgaatgata aaattcattc aattaataac acatatatta
    21181 attgccgtta aaactaaaaa agcatcaata atcaacgtga tataataaac ctgccttaca
    21241 tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg
    21301 gataaataaa taatttattt ttatcactat tatttatgta atcatcctgt cagggagagg
    21361 gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt
    21421 tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc cgctcttcct
    21481 ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga
    21541 tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg
    21601 taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg
    21661 ccgctttttt acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga
    21721 ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc
    21781 tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg
    21841 tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca ctgcgccggt
    21901 aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca
    21961 attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg
    22021 taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa
    22081 accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc
    22141 ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc accaaattca
    22201 tcggggcgca ccgaccattc cgtcagttcc ccctgaatat caccaggcgc atgtagtttc
    22261 agtccatcgc tggcagctgt ttcatcagcc aggcgctcga ccaccggaat aaactggatg
    22321 aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg
    22381 cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca
    22441 cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc
    22501 tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac
    22561 catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca
    22621 ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag taacgttggt
    22681 tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga
    22741 cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg
    22801 gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt
    22861 ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg
    22921 aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg
    22981 cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg
    23041 ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt
    23101 tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct
    23161 ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc
    23221 tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg
    23281 gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg
    23341 agctatagcc accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc
    23401 cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag
    23461 cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact
    23521 tcccacccct ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg
    23581 ttttgctgat tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt
    23641 ataaatcgcc gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc
    23701 ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc
    23761 ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg
    23821 aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc
    23881 actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc
    23941 aattagcaga atagcaatag atactgccac acccgcaacg ggtacgccgt gacgggaaac
    24001 tttcgccatc gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca
    24061 actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat
    24121 gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct
    24181 gcctatttca ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat
    24241 caggatccgc cacagcacct tgcctacggc actgcgcagc gtcacctgcg gattcttcgc
    24301 ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag
    24361 cgcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct
    24421 aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca gaccaatgac
    24481 aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta
    24541 caaccgcacc gccgccagat tcgccaacgc caccagcgcc actgcgatca atgcgggtat
    24601 ccactgcgcc atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga
    24661 gatccccacc gccatccaca taaaccaata agaccaggcg gtgagataac caaagaacgg
    24721 gctcatataa cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat
    24781 ttcgcccatt gaacgcatga tgaaaaagac gaatagcccg gcgatgatat aggccaacaa
    24841 tacggatggc ccggcccatt tcaaggtact ggcggccccc ataaacaggc cgacgccaat
    24901 ggtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg
    24961 tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat
    25021 cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa
    25081 gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact
    25141 ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac
    25201 gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact
    25261 ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt
    25321 aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga
    25381 agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa
    25441 tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga
    25501 tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac
    25561 cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga
    25621 gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta
    25681 caacgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg
    25741 cgttatcttc aatggtcagc attttttcgg cattaatggc aaccagcgtt ccctgcttaa
    25801 gctgcccgtc agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact
    25861 gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac
    25921 ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca
    25981 gtacaacagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg
    26041 tgagacaaac gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa
    26101 gcagaaactg tgcaatatcg cagccttata gcgattagtc tcgcggttgc ccagctcata
    26161 cagccagtcg aaccatttga tgatcagtcc aaccacaatc gccccgagtg ggatgaacaa
    26221 cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg
    26281 gttattcagc acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgacc
    26341 cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg
    26401 gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccatg gcgagaaggt
    26461 gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata
    26521 gcgttttagc gccagccaga acatgcccac aatccccagc acgcccgccg ccgccagcat
    26581 ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat
    26641 gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct
    26701 gacgaggaaa aacagccacg ctttgctatc ctggcgcaga aagtagacca ccagcatcgc
    26761 cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat
    26821 ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac
    26881 gcttaccagc gcgatgccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat
    26941 ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt
    27001 gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg
    27061 cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa
    27121 aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa
    27181 ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacaa caaacaggcc
    27241 actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc ccgctgccag
    27301 ccttgtagat agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg
    27361 cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa
    27421 tgctgttccg tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc
    27481 gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca
    27541 agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt
    27601 tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct
    27661 tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga
    27721 tgaacggcgc acaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga
    27781 atggtcattt tcccttcacg ttgccgatcg ttcgccatcg tattgagaga agggtccatc
    27841 cgcgtcggga agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc
    27901 aaatcgccgc gggcggcaaa tacacagccg actcgctttt gcgccaggcg acgtagtggg
    27961 taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc
    28021 cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg
    28081 aactgaccgt ggaagaaaaa acgctgctga cggttagctt ttgctttcgc gatgaccgct
    28141 tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt
    28201 aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcagccagc
    28261 gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg
    28321 tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa
    28381 aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga
    28441 ttagccagtg ggcaaatacc atcaataaag tgaactggct gatttccgcc agaatataaa
    28501 accgcaatga cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca
    28561 catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca
    28621 gcagccagat agcaaaatca cgcagcagcc agacggtaaa actcgccgcc gccactgccg
    28681 gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc
    28741 gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat
    28801 cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca
    28861 gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga
    28921 gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta
    28981 acgggatgac accacgtttg atcaacatga tagcggcagg aattaccacc agcgcgggaa
    29041 tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg
    29101 cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa
    29161 agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca
    29221 ctaaacgcac cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag
    29281 ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag
    29341 tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta
    29401 caccgttaaa gatgccagct ccggcaagca cgccgagcac ggtaatcaac tggcggaaat
    29461 ttgccgccag cccaagcccc gccggaccaa atgacacagc cagcaactta ccgaccagta
    29521 acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg
    29581 acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac
    29641 aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct
    29701 tcaccgtgga actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg
    29761 aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata
    29821 tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc
    29881 agctcgatac gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc
    29941 gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct
    30001 tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga
    30061 ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt
    30121 aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt
    30181 tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg
    30241 gacatcacgc cctgagcggc atcttctacc acaaacagat tatgcttttt cgccaacgcc
    30301 ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt
    30361 ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca
    30421 tccacaaaaa cgatttttgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg
    30481 tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc
    30541 agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc
    30601 cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc
    30661 gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga
    30721 tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt
    30781 aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata
    30841 cgcccagttt agcgccgttt gcatcagctc agcacctaca ccgcgtccag ccagcaggcc
    30901 aattcgcgca tctgtcgcat tgagttcccg taaagagaca taaccacgaa taccgccgga
    30961 cgccgcacgt aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca
    31021 ctgtgcataa aagcgtctgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg
    31081 cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac
    31141 agcaccacta tcactgacat tgttcacggg tagtgccaaa tcaacttcac cttctaccag
    31201 ggagaatccc agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc
    31261 ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt cagacgtaat
    31321 gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact
    31381 ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg
    31441 aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt
    31501 ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt
    31561 caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa
    31621 cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac
    31681 gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc
    31741 cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga
    31801 aattgtcgtc aaactccacc acgccaaagc gttccgggtc catcacctga tagccaaata
    31861 ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga
    31921 agaagatgtt atcgcccagc accagacaag aaggttcacc gttgaggaag gtttcaccga
    31981 tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac
    32041 cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga
    32101 tgatgagaat ttcgcggata ccggccaaca tcagcaccga cagcggatag taaatcattg
    32161 gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg
    32221 tgccggaacc gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt
    32281 tcagacctaa acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat
    32341 tagccagata ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc
    32401 aaccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac
    32461 ggtcagcgac aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc ttgttcggag
    32521 ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt
    32581 gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc
    32641 gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca
    32701 gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt
    32761 aaggaccgta gttattcgag cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc
    32821 aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat
    32881 acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg
    32941 tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag
    33001 cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg
    33061 ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct
    33121 gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg
    33181 caaagtgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct
    33241 tatcgaccac taccaccgca tcgctcgttt cgttgatgat ataacgcacc agcgccgagc
    33301 caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc
    33361 atcgacgaca tactgctgat ggacattgtc gccattgata actttgaact gactatgatc
    33421 gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca
    33481 aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca gcgtttcgcc
    33541 gctgtgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc
    33601 aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc
    33661 ggtagcagca aggcagtcgg caaccgccgc tttcacctga tcgataaccc agaacggttt
    33721 gtgatcgtta acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac
    33781 gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat
    33841 attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc
    33901 ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc
    33961 ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa
    34021 aattttgtac agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg
    34081 atcgttttta atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc
    34141 aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc
    34201 tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac
    34261 cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat
    34321 atctggctca tgatcgcctt taaacggcgt gggtacagca atcagccagg catccgcttc
    34381 aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc
    34441 caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt
    34501 gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg taggcagccc
    34561 gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt
    34621 tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg
    34681 ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc
    34741 tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg
    34801 gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct
    34861 gagtcggtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg
    34921 ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg
    34981 ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct
    35041 gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc
    35101 accagaatca tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc
    35161 agcgtgtcgc tgctcatcac ctgatcacgc acccataaca gtgcatcaat gactgtatta
    35221 ccagtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt
    35281 tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc
    35341 ggccacggcg aatagagatc gcccgtgcgt aaaccagcct caacgtgacc aacaggaata
    35401 cgctgataaa acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc gtgaaccagc
    35461 acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt
    35521 atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa
    35581 aagagtttca gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc
    35641 tcaaaaaaag gatcttttgc caacgcatgc accaacggag ccatcttgat ggcttccggg
    35701 cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc
    35761 tttgcgctcg cagcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc
    35821 cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc
    35881 gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag
    35941 ggttggacca acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa
    36001 ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag
    36061 gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt
    36121 atgctgctca gcaattttca gcgcctgctc aatgctgttc atccggcggt cgtagatggc
    36181 tttcgccacc tcttcctgac gcttcacctg agctttcatc tggatggtgc gtgccgccca
    36241 tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata
    36301 ctgacgtaac aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt
    36361 gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa
    36421 cgccgcatcg gctttgctgt tgcccaccat ccgctgcttg taatagtcgg tttgcagcca
    36481 gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc
    36541 catgaccgat ggttggtcgg cagaagccat gtttgaacgg gcatccaggt tacgcaaaaa
    36601 ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt
    36661 cgcgctccac tcttgtcgag caaaaaaagt atacgccagc gcgattaacg caaacgccag
    36721 ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc
    36781 cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc
    36841 atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc
    36901 ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct
    36961 agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg
    37021 ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg acgggaagta
    37081 aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg
    37141 cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc
    37201 cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc
    37261 cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg
    37321 cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag
    37381 caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac
    37441 gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta
    37501 atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc
    37561 caggagccaa agatataacc caggctactg agataaagtt tgccgaacac catcataaca
    37621 atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc
    37681 agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata
    37741 tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg
    37801 agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt
    37861 tttgccactt tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga
    37921 tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag
    37981 tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg
    38041 caaaaattaa ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc
    38101 ctaacatcgc cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac
    38161 gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat
    38221 tggtcttagt catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg
    38281 ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct
    38341 cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga
    38401 tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt
    38461 cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct
    38521 cttccacgtt acgcgccgca ccgaagaagc gtttcggacg atgaagggca ttggcgtcca
    38581 caccaccggt caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac
    38641 gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct
    38701 tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag
    38761 caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga
    38821 tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct
    38881 gcagcagcat agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac
    38941 cgataggtga tgccagatcc agtacgcgag cggttaaatc ttcagtagaa ccgttaccac
    39001 gttccatacg cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt
    39061 tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc
    39121 gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga
    39181 aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt
    39241 ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga
    39301 tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca
    39361 tacgagccag gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg
    39421 tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg
    39481 aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca
    39541 tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga
    39601 gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt
    39661 acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact
    39721 cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag
    39781 gttagcgtcg aggaactctt tcaactgacc tttagacagt gcacccactt tggttgccgc
    39841 cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc
    39901 agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc
    39961 agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa
    40021 atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag
    40081 gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg
    40141 ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta
    40201 aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa
    40261 gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca
    40321 taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc
    40381 cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta
    40441 tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccacgtg ccttaattat
    40501 ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc
    40561 taccggcctg aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt
    40621 gctggaaagc ggcgttgaca ttctgattgg cactacgggg cgtttaattg actacgccaa
    40681 gcagaaccac attaacctcg gtgccattca ggtggtggta ctggacgaag ccgatcgcat
    40741 gtacgatctg ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa
    40801 ccagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt
    40861 cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg
    40921 tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct
    40981 gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga
    41041 agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt
    41101 cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct
    41161 ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa
    41221 ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc
    41281 aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc
    41341 tattgagacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat
    41401 gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg
    41461 tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca
    41521 ttcaaactgt atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat
    41581 tcaggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca
    41641 cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc
    41701 gcgaggtggc tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg
    41761 ctggcctgaa tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc
    41821 tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta
    41881 cggcgacatt acgtcttgcc gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc
    41941 tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg
    42001 ttgctcacac aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta
    42061 ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct
    42121 gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg
    42181 ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg
    42241 gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg
    42301 cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca
    42361 ttcattgcgg tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag
    42421 tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta
    42481 tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg
    42541 tcgagcagga tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata
    42601 ttgatcaggc gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg
    42661 aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa
    42721 tcggcctgag cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc
    42781 tggatcttcc cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc
    42841 agactaatcc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg
    42901 cagaacaact ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg
    42961 atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc
    43021 aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc
    43081 agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg
    43141 acgcatcttt tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt
    43201 cgccagatgg ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg
    43261 ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac
    43321 cagctcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg
    43381 ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct
    43441 ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc
    43501 ttttgacgcg tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg
    43561 ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc
    43621 cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat
    43681 gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat
    43741 gccgtggatc agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc
    43801 tgccaaccag tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact
    43861 caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc
    43921 cagctttttc agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa
    43981 tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc
    44041 aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt tttgcatcag
    44101 gaatttttca aacactcgcg actgatggtt accgcgataa agaatagcgt aatctttgta
    44161 ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct cggcttcatg
    44221 ttcttcgtta ttcgcgctta aaactttaag ctctgtgccg taacccagtt cggagaacag
    44281 acgcttctca aagacgtgcg ggttattggc gatcaggatg ttcgccgctt tcaaaatacg
    44341 tccggaagag cggtagttct gctcaagctt aatcaccgtc agcgccggaa aatcctgact
    44401 cagcagcacc aggttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc
    44461 gcccaccacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagct catactggct
    44521 ggtgtttgtg tcctgatact catccaccag cagatagcga attttgttct gccagcgctc
    44581 gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc
    44641 gagaacattg cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg
    44701 atcccgctcg ccctttgcct ctgccgccgc ctgcgccgga gttttgagat cgttcttcca
    44761 gttggagatg gtcgaaatca gctgttgcag gagaactttg tcatcttcaa tcagcccctc
    44821 ggtcaactct ttaagcaaag caagctgatc ggtatcgtca aacagcgaaa agttcgcctt
    44881 catcccaagc gccgcatact cgcgtttgat gatatccaac cccagcgtgt gaaaagtgga
    44941 gatcatcagc ccgcgcgcct ctttgcgacc cagcgtctgc ccgacacgct ctttcatctc
    45001 gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc
    45061 gcggatcaga tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc
    45121 cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg
    45181 cataggtgta ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat
    45241 gtgaccgcgt gtaagccgct aaccggttgg ctgcggatgt ttaaatcaga gagagaagac
    45301 cgaaatcgga gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg
    45361 ttattacttt ttcagagttt actaaggaga ggtttactcg tggcgattaa tttcagccct
    45421 aaggttggtg aaatacagga atgcaacttt ggtaactatc ctgttatcca gaatggacag
    45481 ttcagcacca cgtattacga tggccgcatt cctcctgaaa tgataaaaaa caggttagtt
    45541 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
    45601 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
    45661 gtcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
    45721 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
    45781 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
    45841 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
    45901 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
    45961 ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
    46021 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
    46081 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
    46141 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
    46201 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
    46261 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
    46321 cgcaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
    46381 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaacagc aatacgtttc
    46441 atatctgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gctgcgaatc
    46501 gcttcgttca catcacgcag ttgcgcgtta tctaccgcgc cttccggaat agctttaccc
    46561 aagtcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
    46621 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
    46681 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
    46741 tcttcaatga tgccggaatc gaccatggtt tcgaacgcca gttcaacgcc cgctttcacc
    46801 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
    46861 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
    46921 tcatcgttgg cccagtccgc catcataccg gaggagaact cgccggagat gatgtcgtcc
    46981 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
    47041 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
    47101 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
    47161 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
    47221 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
    47281 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat accttcgcct
    47341 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
    47401 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatatcttta
    47461 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
    47521 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg
    47581 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
    47641 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
    47701 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
    47761 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
    47821 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
    47881 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
    47941 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
    48001 cattgcaatt tctgcaacgt caacattgag ggctgcgcca ttggatttac gcgatctgaa
    48061 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
    48121 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
    48181 atttgtgcgc gataaccgca cggtaacgct gaccgaagcg ggcgaagagt tacgcgtttt
    48241 cgcccagcaa acattgttgc agtatcagca actgcgccac accatcgatc agcaagggcc
    48301 gtcgctctct ggcgagttac atattttctg ctcggtgacc gccgcttaca gccatctacc
    48361 gccgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
    48421 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
    48481 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
    48541 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
    48601 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
    48661 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
    48721 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
    48781 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
    48841 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
    48901 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
    48961 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
    49021 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
    49081 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
    49141 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
    49201 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
    49261 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
    49321 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
    49381 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
    49441 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
    49501 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
    49561 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt
    49621 tgtgctgggc gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca
    49681 ccgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
    49741 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta
    49801 ccgctacgcc ttcagcaaat agccctacgc gcggcagatc aaccggatga cccgcatcca
    49861 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
    49921 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
    49981 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
    50041 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
    50101 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
    50161 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
    50221 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
    50281 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
    50341 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
    50401 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
    50461 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
    50521 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
    50581 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
    50641 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
    50701 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
    50761 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
    50821 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
    50881 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
    50941 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
    51001 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
    51061 gacgcccgcg gttttaacga tgcagccgtt ttctgcgaaa ttaccgtaga gcaccgccag
    51121 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccattgg cgcgatcgtc
    51181 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtggtacgaa tgcctgcagg
    51241 acctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
    51301 cagcgtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
    51361 gcgatccagt tcgccgagaa taccgataac accaccagca cggtgaacat cttccatatg
    51421 gtatttctgg gtgctcggcg caactttaca cagctgtgga accttgcggg aaagcttatc
    51481 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
    51541 ggtgttagtc gatccaccca tcgcgatatc cagcgtcatg gcgttttcaa acgccgcttt
    51601 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
    51661 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtcggcgt gggttgccag
    51721 cagcgagccg ttgcccggct gcgacagacc cagcgcttcg gtcaggcagt tcattgagtt
    51781 agcggtaaac atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc
    51841 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
    51901 tttgattatc tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa
    51961 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
    52021 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
    52081 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tggcaatccc
    52141 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
    52201 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
    52261 gttcacaacc gcgataatcg gcttaccgaa atcggcgtcg gtcattccgg tggcgcgcca
    52321 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
    52381 catactttat ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt
    52441 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
    52501 agaggccgaa gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa
    52561 cctgaatacc gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
    52621 cttcatccgc caggtacagg gattcgcgcg acagaacctg ctcacgcaca tcaattccca
    52681 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga
    52741 acggtggggt gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag
    52801 agatataacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
    52861 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
    52921 ttggtgctgc gcgcttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
    52981 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
    53041 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
    53101 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg
    53161 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
    53221 ggaatacaac cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc
    53281 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
    53341 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
    53401 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
    53461 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
    53521 atatttatat tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca
    53581 cgatgacgca ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca
    53641 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
    53701 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
    53761 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
    53821 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
    53881 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
    53941 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
    54001 cattcatcat gaaagagccg tcaccggaga tacagacgac agtatcgttc ggtcgtgcga
    54061 cttgtgcgcc aaccgccgct ggtaaaccga aacccatggt gcctaagccg ctggaggtaa
    54121 tgaaattttc cgggcgagtg tgtgcgatgt gctgcgcggc ccacatctgg tgctgcccca
    54181 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaatg
    54241 gcgcgtagat agcgtcaccg ggatggtcgt aacgccaggc atgttcatca cgcagctgcg
    54301 cgcagtgtag ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
    54361 ttaaatcacc ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
    54421 tatccatatg gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt
    54481 catcaaaacg tgcacccacg gcgatcagca agtcgcactc ctgcaccgcg aagttcgccg
    54541 ctttggtgcc atgcattccc agcatgccca gatagtacgg ataatctgct tcaactgcgc
    54601 ccagcccttt cagcgtgcag gtggcaggca tttttgtggt agcgagaaat tctcgtaaag
    54661 caggaactgc ctgcgccata cccacgccac caccaacgta cagcatcggt ttttgcgctt
    54721 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
    54781 cggtggtgaa ccacggttcc aggtcaccgc tggctagctg gatatctttt gggatatcga
    54841 ccagaaccgg accaggacga cctgagctgg caacgtcgaa tgcttcagcc ataatgcgcg
    54901 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccca
    54961 ggacatccac ttcctgaaat gcgtccgtgc cgataaacgg tgcggacact tgaccggtga
    55021 tggcaacaac agggatagaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
    55081 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
    55141 tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat
    55201 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
    55261 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
    55321 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
    55381 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
    55441 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
    55501 ctaatcacga ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    55561 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttt
    55621 ggccgatttt tatgatgcgt ttaataattt caatagaaat tgttgttttt taaaaagaaa
    55681 atagtgagtg aaaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat
    55741 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
    55801 gcacaaataa acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata
    55861 tcttatgcac ataatagtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
    55921 cgcagccctg ggagtaaatg cgcccccaat cactgttgag gtacatatca gtaaaggtct
    55981 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    56041 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    56101 ggctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    56161 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    56221 actggcgctt acaggcgctc tgcgtggcgt tcccggcgca atctccagtg caactgaagc
    56281 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    56341 taacggtgaa ggatgcctga tagccgatca tctgcaggct gtctgtgcgt ttctggaagg
    56401 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    56461 tctcagtgat gttgtcggtc aggagcaagg aaagcgagga ctggaaatta ccgccgctgg
    56521 cgggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    56581 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
    56641 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    56701 gcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga
    56761 aatttcgttg gcgcataacg gcgtgctttt tcttgatgag ctacctgaat ttgaacggcg
    56821 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacacg
    56881 agccaaaata acctatccag cccgtttcca gcttgttgcg gcgatgaatc ccagccctac
    56941 cggacattat cagggaaacc ataaccgctg cacgccagaa cagacattac gttatctcaa
    57001 ccggctctcg gggccctttc tcgaccgctt cgatctctca ctggagatcc cattaccacc
    57061 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    57121 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    57181 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaagg
    57241 aacactgatc catctggggt tatcgattcg tgcctggcag cggttattga aagttgcacg
    57301 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    57361 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    57421 gggcatttcg ccctttttat taatcatcag aatcggtgta gtcttcagca ccttcaacct
    57481 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    57541 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    57601 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    57661 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    57721 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    57781 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    57841 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    57901 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    57961 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    58021 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    58081 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    58141 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
    58201 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
    58261 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    58321 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    58381 gatatttcac tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt
    58441 atctgcgact tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    58501 acgaagtgcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    58561 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    58621 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    58681 aaaatacgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg
    58741 aatggctggc aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa
    58801 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    58861 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    58921 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    58981 aactaccgct ccaccgaatt cttttacaac caccggtttt atgaccggct tactgcttaa
    59041 tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta
    59101 cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct acggccgcca
    59161 ggcaaattct gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa
    59221 atctttctct catccgccaa aaca
//
LOCUS       DAZGCS010000017        55085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGCS010000017 DAZGCS010000000
VERSION     DAZGCS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.017"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..612
                     /locus_tag="ACHK01_001120"
                     /pseudo
     CDS             6..612
                     /locus_tag="ACHK01_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            715..1218
                     /gene="yfbM"
                     /locus_tag="ACHK01_001121"
     CDS             715..1218
                     /gene="yfbM"
                     /locus_tag="ACHK01_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO8096102.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1285..2742)
                     /gene="nuoN"
                     /locus_tag="ACHK01_001122"
     CDS             complement(1285..2742)
                     /gene="nuoN"
                     /locus_tag="ACHK01_001122"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO8096103.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2749..4278)
                     /gene="nuoM"
                     /locus_tag="ACHK01_001123"
     CDS             complement(2749..4278)
                     /gene="nuoM"
                     /locus_tag="ACHK01_001123"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO8096104.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4442..6283)
                     /gene="nuoL"
                     /locus_tag="ACHK01_001124"
     CDS             complement(4442..6283)
                     /gene="nuoL"
                     /locus_tag="ACHK01_001124"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO8096105.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6280..6582)
                     /gene="nuoK"
                     /locus_tag="ACHK01_001125"
     CDS             complement(6280..6582)
                     /gene="nuoK"
                     /locus_tag="ACHK01_001125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO8096106.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6579..7133)
                     /gene="nuoJ"
                     /locus_tag="ACHK01_001126"
     CDS             complement(6579..7133)
                     /gene="nuoJ"
                     /locus_tag="ACHK01_001126"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO8096107.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7145..7687)
                     /gene="nuoI"
                     /locus_tag="ACHK01_001127"
     CDS             complement(7145..7687)
                     /gene="nuoI"
                     /locus_tag="ACHK01_001127"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO8096108.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7702..8679)
                     /gene="nuoH"
                     /locus_tag="ACHK01_001128"
     CDS             complement(7702..8679)
                     /gene="nuoH"
                     /locus_tag="ACHK01_001128"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO8096109.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8676..11402)
                     /gene="nuoG"
                     /locus_tag="ACHK01_001129"
     CDS             complement(8676..11402)
                     /gene="nuoG"
                     /locus_tag="ACHK01_001129"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO8096110.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11455..12792)
                     /gene="nuoF"
                     /locus_tag="ACHK01_001130"
     CDS             complement(11455..12792)
                     /gene="nuoF"
                     /locus_tag="ACHK01_001130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO8096111.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12789..13289)
                     /gene="nuoE"
                     /locus_tag="ACHK01_001131"
     CDS             complement(12789..13289)
                     /gene="nuoE"
                     /locus_tag="ACHK01_001131"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO8096112.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13292..15094)
                     /gene="nuoC"
                     /locus_tag="ACHK01_001132"
     CDS             complement(13292..15094)
                     /gene="nuoC"
                     /locus_tag="ACHK01_001132"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO8096113.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15188..15850)
                     /gene="nuoB"
                     /locus_tag="ACHK01_001133"
     CDS             complement(15188..15850)
                     /gene="nuoB"
                     /locus_tag="ACHK01_001133"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO8096114.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15866..16309)
                     /gene="nuoA"
                     /locus_tag="ACHK01_001134"
     CDS             complement(15866..16309)
                     /gene="nuoA"
                     /locus_tag="ACHK01_001134"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO8096115.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16940..17878)
                     /gene="lrhA"
                     /locus_tag="ACHK01_001135"
     CDS             complement(16940..17878)
                     /gene="lrhA"
                     /locus_tag="ACHK01_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO8096116.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            18798..20015
                     /gene="alaA"
                     /locus_tag="ACHK01_001136"
     CDS             18798..20015
                     /gene="alaA"
                     /locus_tag="ACHK01_001136"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO8096117.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20099..20698
                     /gene="yfbR"
                     /locus_tag="ACHK01_001137"
     CDS             20099..20698
                     /gene="yfbR"
                     /locus_tag="ACHK01_001137"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8096118.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20757..22589)
                     /gene="yfbS"
                     /locus_tag="ACHK01_001138"
     CDS             complement(20757..22589)
                     /gene="yfbS"
                     /locus_tag="ACHK01_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO8096119.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22676..23326)
                     /gene="hxpA"
                     /locus_tag="ACHK01_001139"
     CDS             complement(22676..23326)
                     /gene="hxpA"
                     /locus_tag="ACHK01_001139"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO8096120.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23337..23831)
                     /gene="yfbU"
                     /locus_tag="ACHK01_001140"
     CDS             complement(23337..23831)
                     /gene="yfbU"
                     /locus_tag="ACHK01_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO8096121.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23914..24369)
                     /gene="yfbV"
                     /locus_tag="ACHK01_001141"
     CDS             complement(23914..24369)
                     /gene="yfbV"
                     /locus_tag="ACHK01_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO8096122.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24707..25909
                     /gene="ackA"
                     /locus_tag="ACHK01_001142"
     CDS             24707..25909
                     /gene="ackA"
                     /locus_tag="ACHK01_001142"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO8096123.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25984..28128
                     /gene="pta"
                     /locus_tag="ACHK01_001143"
     CDS             25984..28128
                     /gene="pta"
                     /locus_tag="ACHK01_001143"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8096124.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28318..29838
                     /gene="yfcC"
                     /locus_tag="ACHK01_001144"
     CDS             28318..29838
                     /gene="yfcC"
                     /locus_tag="ACHK01_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO8096125.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29871..30413)
                     /gene="yfcD"
                     /locus_tag="ACHK01_001145"
     CDS             complement(29871..30413)
                     /gene="yfcD"
                     /locus_tag="ACHK01_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO8096126.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30471..31022)
                     /gene="yfcE"
                     /locus_tag="ACHK01_001146"
     CDS             complement(30471..31022)
                     /gene="yfcE"
                     /locus_tag="ACHK01_001146"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO8096127.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31078..31722)
                     /gene="yfcF"
                     /locus_tag="ACHK01_001147"
     CDS             complement(31078..31722)
                     /gene="yfcF"
                     /locus_tag="ACHK01_001147"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO8096128.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31858..32505
                     /gene="yfcG"
                     /locus_tag="ACHK01_001148"
     CDS             31858..32505
                     /gene="yfcG"
                     /locus_tag="ACHK01_001148"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO8096129.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32562..32924
                     /gene="folX"
                     /locus_tag="ACHK01_001149"
     CDS             32562..32924
                     /gene="folX"
                     /locus_tag="ACHK01_001149"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO8096130.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32945..33838
                     /gene="yfcH"
                     /locus_tag="ACHK01_001150"
     CDS             32945..33838
                     /gene="yfcH"
                     /locus_tag="ACHK01_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO8096131.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33886..34776)
                     /gene="rpnB"
                     /locus_tag="ACHK01_001151"
     CDS             complement(33886..34776)
                     /gene="rpnB"
                     /locus_tag="ACHK01_001151"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO8096132.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIAHKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34973..35746)
                     /gene="hisP"
                     /locus_tag="ACHK01_001152"
     CDS             complement(34973..35746)
                     /gene="hisP"
                     /locus_tag="ACHK01_001152"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO8096133.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35754..36470)
                     /gene="hisM"
                     /locus_tag="ACHK01_001153"
     CDS             complement(35754..36470)
                     /gene="hisM"
                     /locus_tag="ACHK01_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8096134.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36467..37153)
                     /gene="hisQ"
                     /locus_tag="ACHK01_001154"
     CDS             complement(36467..37153)
                     /gene="hisQ"
                     /locus_tag="ACHK01_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO8096135.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37243..38025)
                     /gene="hisJ"
                     /locus_tag="ACHK01_001155"
     CDS             complement(37243..38025)
                     /gene="hisJ"
                     /locus_tag="ACHK01_001155"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO8096136.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38246..39028)
                     /gene="argT"
                     /locus_tag="ACHK01_001156"
     CDS             complement(38246..39028)
                     /gene="argT"
                     /locus_tag="ACHK01_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO8096137.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39294..39863)
                     /gene="ubiX"
                     /locus_tag="ACHK01_001157"
     CDS             complement(39294..39863)
                     /gene="ubiX"
                     /locus_tag="ACHK01_001157"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO8096138.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39958..41475)
                     /gene="purF"
                     /locus_tag="ACHK01_001158"
     CDS             complement(39958..41475)
                     /gene="purF"
                     /locus_tag="ACHK01_001158"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO8096139.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41512..42000)
                     /gene="cvpA"
                     /locus_tag="ACHK01_001159"
     CDS             complement(41512..42000)
                     /gene="cvpA"
                     /locus_tag="ACHK01_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO8096140.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42259..42921)
                     /gene="dedD"
                     /locus_tag="ACHK01_001160"
     CDS             complement(42259..42921)
                     /gene="dedD"
                     /locus_tag="ACHK01_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO8096141.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42911..44179)
                     /gene="folC"
                     /locus_tag="ACHK01_001161"
     CDS             complement(42911..44179)
                     /gene="folC"
                     /locus_tag="ACHK01_001161"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO8096142.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44249..45163)
                     /gene="accD"
                     /locus_tag="ACHK01_001162"
     CDS             complement(44249..45163)
                     /gene="accD"
                     /locus_tag="ACHK01_001162"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO8096143.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45319..45978)
                     /gene="dedA"
                     /locus_tag="ACHK01_001163"
     CDS             complement(45319..45978)
                     /gene="dedA"
                     /locus_tag="ACHK01_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8096144.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46061..46873)
                     /gene="truA"
                     /locus_tag="ACHK01_001164"
     CDS             complement(46061..46873)
                     /gene="truA"
                     /locus_tag="ACHK01_001164"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO8096145.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46873..47886)
                     /gene="usg"
                     /locus_tag="ACHK01_001165"
     CDS             complement(46873..47886)
                     /gene="usg"
                     /locus_tag="ACHK01_001165"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8096146.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47952..49088)
                     /gene="pdxB"
                     /locus_tag="ACHK01_001166"
     CDS             complement(47952..49088)
                     /gene="pdxB"
                     /locus_tag="ACHK01_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO8096147.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49187..50182
                     /gene="flk"
                     /locus_tag="ACHK01_001167"
     CDS             49187..50182
                     /gene="flk"
                     /locus_tag="ACHK01_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO8096148.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50179..51357)
                     /gene="yfcJ"
                     /locus_tag="ACHK01_001168"
     CDS             complement(50179..51357)
                     /gene="yfcJ"
                     /locus_tag="ACHK01_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096149.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51641..52861)
                     /gene="fabB"
                     /locus_tag="ACHK01_001169"
     CDS             complement(51641..52861)
                     /gene="fabB"
                     /locus_tag="ACHK01_001169"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO8096150.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53020..55026
                     /gene="mnmC"
                     /locus_tag="ACHK01_001170"
     CDS             53020..55026
                     /gene="mnmC"
                     /locus_tag="ACHK01_001170"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO8096151.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT        13700 a        15078 c        13821 g        12486 t
ORIGIN      
        1 tatttctgat ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc
       61 aacgccccga cacgtcagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
      121 tgtgcatcca cgtaatgccg acaatattga taatctgaat aggtccgtag agtatataaa
      181 agagttcttt gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt
      241 acgactctgc gcctggtagc caggattacc cttacccgca atgtcctggc tttatcccga
      301 tcggggggac tttattgcct ggtcggcaga atacaggata tcaacgccgt tcgtcaggta
      361 aaaacggcat tgttatcatc ccgggattta tctgtttatt ctatgaatgc cccatggttt
      421 attcccggta ttgatttctc tgaccacctg aattattggc aacacgatat tcccgccgta
      481 atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgatacagca
      541 gacagattga attatcagaa aatggctcag gtggtggatg gggttataac tttgttatac
      601 aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa aagcgacatg cacattttat
      661 tttccctcgt ttattttaag atgcgaaaca taaaattcaa cggaggaaaa ataaatggga
      721 atgattggct attttgcaga aattgactca gaaaaaatta accaactgct tgagtctact
      781 gaaaaaccct taatggacaa tattcatgac actctttcag gattacgccg actggatatt
      841 gataaacgct gggatttttt acactttggg ctgacaggca cctctgcttt tgatcccgcg
      901 aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga tggcatagat
      961 ggcttcctgg gattgacctg gaatcaggag ttagccgcga caatcgaccg actggaagct
     1021 ttggatcgaa gtgagctgcg taaaaagttt tccattaagc gactcaatga aatggagatt
     1081 tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcttc gatcatgctt
     1141 gatatggaaa agctgattag cgcgtatcga cgtatgctgc gacaggggaa tcatgcgctg
     1201 acggttattg ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg
     1261 ctttctgaat tacgaactga cagcttacat cagcggcatg gctaaccgca caatgctaat
     1321 cagcggttgt ggccatacac ccagcaccag taccaacagt gcagagatca gcacaacgat
     1381 accgcccgcg ctgtactgcc agtttgatgg tgcatcgcga cccggttgtt ccggggcgtg
     1441 aagatacagg ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac
     1501 cacggcaccc accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt
     1561 accgataaag cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac
     1621 tgccgcgagg atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc
     1681 cgggccacga tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa
     1741 cagataaccg gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc
     1801 aatcagcgcc accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt
     1861 gatgttggtc tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat
     1921 cgccagtact acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat
     1981 caccacaccg aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg
     2041 cgcgccctgg tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc
     2101 gaggccaaca atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc
     2161 gccgaggttt ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc
     2221 cataccgaac agcaggaatg aagacgctgc ggcagaaagg atggtgtatt tgatactggc
     2281 ttccagcgaa cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat
     2341 cagttcaata ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc
     2401 cagcgcggca attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag
     2461 ccacgggtag gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag
     2521 catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccgac
     2581 aaaccagagc gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa
     2641 atgattgcgt cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag
     2701 caacggtagc agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt
     2761 agtagtaacg gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc
     2821 cagaatcggc tgcggataga agcccagcag taccagcagc accaccagca acaggatcat
     2881 aaacagctca cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt
     2941 accgaagtaa gcgcgatgta acatcgccag cgaataaaca gatgcaaaga ccagcccaaa
     3001 ggtagagata acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc
     3061 gccgacgaag ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag
     3121 tgctggcagc catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg
     3181 gatacgttca taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc
     3241 aatcatctgg attaccgcgc cctggtaggc caactggctg ccggtgtaga tggcaatcag
     3301 cacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatgt cggtctgggc
     3361 gaaggccatc cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc
     3421 aaactctgcc gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc
     3481 agttttcagc aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc
     3541 atccggcagc cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa
     3601 gcccagcatt aacaggtatt ccacaccact ggacattggc gtattcagca gctcttcata
     3661 gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat
     3721 caacatcacc agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg
     3781 cgttttaccg tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag
     3841 catcatttcc cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac
     3901 gccgcccagg atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt
     3961 ccacgaacag agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga
     4021 cagcccgtca atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa
     4081 ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca
     4141 cagttgcagc gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgcggcac
     4201 cttgacgcca aagcgttcgg tctgccagca caggaagccg ccgataaagg gaattaatat
     4261 tagccagggt agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt
     4321 ttcaacgaat tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg
     4381 cgaatgcggc ttatccgacc tacgttctta tatgccaagg ggcaatccca caatccttaa
     4441 ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca
     4501 cataccagcg cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa
     4561 ggacagccgg gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac
     4621 ccaggaatgg cttgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc
     4681 accaggtgcc cagcagacgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt
     4741 tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa
     4801 tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca
     4861 gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca
     4921 ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga
     4981 tgaaaatcat acggaaggtg tagagcgagg tcataaacgc acccaccaga cctgccacca
     5041 tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc
     5101 ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa
     5161 ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg
     5221 ccagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca
     5281 agtggaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct
     5341 ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac
     5401 cggccagcag cagcgtaacc gccccaacaa tgcccaccag atgcagaact tccggggtca
     5461 tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg
     5521 cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca
     5581 acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca
     5641 gcatgttatt gccatcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca
     5701 gggtgcccag ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac
     5761 gggtcacgac gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac
     5821 cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt
     5881 tgtcggcaag caccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc
     5941 gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta catgtggatc aggaaaccca
     6001 caccagtgac cactgagagc atggtcagcg acaggccgtc caacaccagg ttaaaaccga
     6061 tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt
     6121 tagcgaagaa atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa
     6181 cgccgacgat cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga
     6241 agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac
     6301 tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat
     6361 actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg
     6421 accccagtag cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag
     6481 accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa
     6541 taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt
     6601 cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg
     6661 acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt
     6721 acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata
     6781 ccctgatcgt taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa
     6841 attgccggac caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa
     6901 ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag
     6961 acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc
     7021 gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact
     7081 cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct
     7141 ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct
     7201 tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc
     7261 ccggaccgga gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc
     7321 ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac
     7381 cacagaaaat gcagcgtgag aagttgatac ggaaaaattc cggataccag cgaccgtctt
     7441 tggtttctgc cttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac
     7501 aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac
     7561 ggggcggcag atagaccggc tcttccgggt acattcgcgt ttcgcgtttt gcgaacgcgt
     7621 gcaggccgat catccagata ctacgaacct gggtgccgaa acctactaac aattctttta
     7681 aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag
     7741 caagttgatc agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata
     7801 acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt
     7861 cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt
     7921 caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa
     7981 cttcataccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg
     8041 gtcaaacggg tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa
     8101 gaattgcggg atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatattgaa
     8161 tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag
     8221 ggtctgcgca gaagcacgca tcgcaccgag cagcgagtat ttgttgttac ttgaccagcc
     8281 cgcaaacagc accgcgtaaa ctgccagacc tgccatcatc aggaagaaca aaatcccgat
     8341 gttcaggtcg gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag
     8401 cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga acttcgggat
     8461 ccagtcttct ttaaagaaca ttttgatcat atccgcaacc aactggagcg aaccgcccca
     8521 gccaacacgg ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa
     8581 gctcatgaat gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt
     8641 cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc
     8701 tcaagatgcg cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc
     8761 tgccctgccg tcagcccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa
     8821 ctaaaggaga cgcgtgtacc tgcgtttaca cccaacttcg cggcatccgc tgggttgagt
     8881 ttgatgtacg gctgcggcat acgactctgg aagaccggag cacgctgtga caattcatca
     8941 ctgccaaaca ggtggtagta cggcgcaata cgccatttcc cgtcctgcgg ctggaagcgt
     9001 gccggtacgc tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc
     9061 ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg
     9121 gagttccagc ccggagccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg
     9181 ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga
     9241 acgctgatat tggcgcgcat ggcggtacgg ccgctgtaac ggtgcggttc acgggccagt
     9301 ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc
     9361 gggattttcg ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg
     9421 ctcagcaggg tgctgtgcag cgagtgtaac caacgccagc tttccagcat gacagttttg
     9481 ctatcgtaat aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta
     9541 attaccgtac cgtcgctttc agcaaagctg gcagcggaaa gcaccagatg ggcgttttcc
     9601 atgatcgctg tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca
     9661 ttcacgcggg tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg
     9721 gcgcgtccgg tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc
     9781 agccccatgc tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct
     9841 ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct ctaagctacc ggcgttcgtc
     9901 ccggagataa tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg
     9961 ctttgcagct caggttcgat accgtcaacc gctggtgcag agttatccag cgcatgggca
    10021 atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata
    10081 tcatccagac gagtgtcatc aacgttggta acaaacagcg gatgcttagc acgttgaccg
    10141 atgttgagga ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc
    10201 gctttacctt tcaccgcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg
    10261 tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa
    10321 atgccgcctt cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg
    10381 ataccggtgt agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg
    10441 ctggcacgcg gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg
    10501 ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc
    10561 tgacgcggac gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg
    10621 aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt
    10681 tcaccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc
    10741 atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg
    10801 caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg
    10861 aagtagacgt tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg
    10921 tagtaacgca cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc
    10981 cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg
    11041 gtcatcacgg tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg
    11101 tgcgggtggt tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg
    11161 tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga
    11221 ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg
    11281 cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct
    11341 tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc
    11401 attagcatgc ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca
    11461 gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg
    11521 tttgattccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac
    11581 tgcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc
    11641 aagtgtttcg atatctcccg gctgaccttc gccacgctcc agcgcacgca gaattttcac
    11701 gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa
    11761 ctcttccaga ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag
    11821 cgccgtaccc agacggcttc ccgctttacc gatactttcg aattccatcg gcaggtcaag
    11881 gtgcgcttca gtcaggaaat cagtccccgc gccgcctggc tgccaggctt tgaatttcag
    11941 accgtcacgc ataccaccgg cgtaatcttc aaggatttca cgcgcggtag taccgaacgg
    12001 cagttcccac aggcccggat ttttcacccg accagagaag cccatcagct tggtgccagc
    12061 atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac
    12121 gttacacagg gtttcgacgt tgttgacgca ggttggttta ccccatgcac cggaggttgc
    12181 cgggaaaggt ggcttcgagc gtgggttagc gcgacgccct tccagggagt tgattaacgc
    12241 tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcaaa
    12301 acctgttccc ataatatttt tgccaagcaa acctgcttcg gtggcttcgg caatggcacg
    12361 gcgcagattc actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc
    12421 tttcagcgca aacgcggaga taagcatacc ttccaccagc aggtgcggca gttgctccat
    12481 cagaaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg
    12541 gatgttcatg gattcgtctt tcggcatcag gctccatttc aggccggtag aaaagcccgc
    12601 gccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag
    12661 cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcgatatt cgtccagcca
    12721 cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat
    12781 aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg
    12841 tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt
    12901 ggcagcagcg taaagcgacc gtcaaatgtc gtttgccctg gtttgatgtt tagctttttc
    12961 tcgagcgccg cctgaatacc ctggtaaccg ttgatatgac agaccacgct gtcgcaataa
    13021 cggatcacat ggcggccaac cggctggcgg aagatctggc tgtagaacgt tgccacgcct
    13081 tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc gccatccggc
    13141 acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct
    13201 tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc
    13261 tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat
    13321 cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa
    13381 tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac
    13441 cgtcgctggt caggtagtaa ctgttgatcc ccttcgtcgc ctcaatcatc tggaaagatt
    13501 cattggcagg catcaccggc ccccacgaca cttgcaagaa gtgggtgatc agggtttcga
    13561 tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg
    13621 ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt
    13681 ccactttcag cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa
    13741 agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg
    13801 tggcacgcag gcccgcgccg gtggtgcccc actccagcgc ctctttcgcg ccataggcgg
    13861 caacgccctg ggaacgacct ttcagaatgg tattttgcag cgctgctttc tcgtaagacg
    13921 ccagacgttt cggcatccag tcgaggaact cacgcagcag gcgatcccag ccacgcggca
    13981 ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg catacggaaa ccagtgattg
    14041 cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacact ggcgtcattg
    14101 cgccgacgtc ctgaataaag gtcgagatat acagcaggtg gctgttgatg cggaacagct
    14161 cagaaagcat tacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct
    14221 ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattcg atacggtcgg
    14281 tatacggaat gtagctgtgc caggactggc gttcgcccat tttctccgca ccacggtggt
    14341 ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc atcgagctgc aaaacgatac
    14401 ggaaagcacc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt
    14461 tttcagtacc acgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca
    14521 gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat
    14581 ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt
    14641 gaccgtcgaa tgtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg
    14701 ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca
    14761 tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag
    14821 gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga
    14881 cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga
    14941 tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac
    15001 ggttgcgcag ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt
    15061 cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg
    15121 cgcagtaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag
    15181 gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct
    15241 tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg
    15301 agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg
    15361 cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc
    15421 cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga
    15481 taacccattt tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg
    15541 taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggggaagca cgcaatactt
    15601 ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac
    15661 agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt
    15721 cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga
    15781 tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg
    15841 tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg
    15901 ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca
    15961 gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc
    16021 agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga
    16081 taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga
    16141 ccgagtcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta
    16201 aaaaccaacc gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag
    16261 cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat
    16321 caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg
    16381 gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg
    16441 gtcttttact cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt
    16501 tacataatat caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc
    16561 aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct
    16621 attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg
    16681 acacgctttt gtcattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc
    16741 attgtagcag gtgatttttt tcaggcgatt atttgtgcgt acggggcgtg aatctctggt
    16801 gattgaaaaa tgaacagttt tgtacgttct gcacgatgtg aagaaggttc tttactaaaa
    16861 aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga
    16921 attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt
    16981 ccggagcaga tattgggctg tattgccacg gattatggta gctctccatc gcctgataga
    17041 tcacctgagc cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcgg
    17101 gtagcggcgg taagccatcg actccactca gcacacgcag gtctggactc atcatctcga
    17161 ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg
    17221 aggcgacata agccagtcgc cagggaatat ctgctttatt aagcgtcgcc agcaccatat
    17281 cgcgaaacgg acttggatca tccagcaata caagagggat cggctcacct ttttgcaata
    17341 cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg
    17401 ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca
    17461 tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc
    17521 gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca
    17581 cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt
    17641 tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac
    17701 gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg
    17761 actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa
    17821 caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat
    17881 attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca
    17941 attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat
    18001 gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat
    18061 cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg
    18121 acatgcgcgt gtttgtataa atgtaaatgt aagtccttgt tccactctcg tgcagcatcg
    18181 ctggtcatac gcgaacacgt acaaacagca atggtgaggc ggcatcaaga gcagggatcc
    18241 gtcatttatg cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta
    18301 tctgatgcaa agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca
    18361 aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct
    18421 taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca cgctgttaaa
    18481 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat
    18541 tttcacacaa ttatcttaca gataaaaaaa ccagacttac aattaagaat cagaacaatt
    18601 cactatataa cattgcatgt aaagcatata cacctcatta ttttgtcatt attaagttat
    18661 taacagcaca atcgagcctt ccactctggc aaaatcttat tctgcagacc ttcaaaacac
    18721 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
    18781 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
    18841 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
    18901 tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc
    18961 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt
    19021 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
    19081 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
    19141 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta tggaccgcgg
    19201 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
    19261 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta
    19321 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
    19381 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct
    19441 acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct
    19501 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
    19561 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
    19621 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
    19681 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
    19741 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
    19801 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
    19861 tcctgttgca ggaaaaagtc ctgttggtgc aagggacggc attcaactgg ccgtggccgg
    19921 atcacttccg cattgtcact ctgccgcgtg tcgatgatat cgagctgtct ttgagtaagt
    19981 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
    20041 atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
    20101 gaaacagagc catttttttg cccatctctc acgcctgaaa ctcattaacc gctggccgct
    20161 aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
    20221 ccatgcgctg gcagctatca aaaatcgtaa atttggcggt aatgtcaacg ccgaacgtat
    20281 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
    20341 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
    20401 gcaaaaactg gtcgatatgg ttccggaaga gctgcaggat atctttgcgc cgttaattga
    20461 cgagcatgca tatagcgatg aagaaaaatc gttggtgaaa caggcagatg cactgtgtgc
    20521 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
    20581 gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt
    20641 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
    20701 agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
    20761 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
    20821 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta
    20881 accggtgaag aaaccggtgt cataaaggcg gcagatgctg ccatcgccac gaccatcgcg
    20941 aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
    21001 accgccgtcg cggtattaga gataaacagc ccaataaccg ccgacaaaac aaacaaacac
    21061 cccagcatca tatgtggccc gtaaccgccg ccaatgtcca tcagcccttt caccgccagc
    21121 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
    21181 atgctcggcc agtgaatgga tttataggcg ctttcggcat cgatacagcg gaatttcccc
    21241 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatcggt cagcattaac
    21301 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
    21361 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt tgccagcata
    21421 ccgatcagtt tccagttacc aacaaccagg atgatatcgc ccagcagcag aggctcatcc
    21481 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
    21541 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
    21601 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
    21661 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
    21721 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
    21781 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
    21841 ttgagatcgt ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc
    21901 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
    21961 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
    22021 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
    22081 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
    22141 atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt
    22201 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
    22261 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
    22321 ctgttgcccg ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
    22381 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
    22441 gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
    22501 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
    22561 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
    22621 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
    22681 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
    22741 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
    22801 aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
    22861 gcggcgcaag ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc
    22921 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc
    22981 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
    23041 tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg
    23101 cctcgatgtg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
    23161 taaaatggcg caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
    23221 gtgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
    23281 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacacgg gcctcctcag
    23341 gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
    23401 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
    23461 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
    23521 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
    23581 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
    23641 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc
    23701 atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt
    23761 gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg
    23821 gtcatttcca ttgttgactc ctgtatcact atactacggt gaaaaaaaag aaggctgagt
    23881 atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc
    23941 cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc
    24001 aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt
    24061 aaggattgca gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat
    24121 gggtaaactt aaggcgaaca gcgcagtggc aactgccggc ccaagctgac cgcccagggc
    24181 aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt
    24241 catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca gcggccacgt
    24301 ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg
    24361 cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca
    24421 actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta
    24481 gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc
    24541 cattggctga aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa
    24601 aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat
    24661 ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt
    24721 agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa
    24781 tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa
    24841 atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga
    24901 agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct
    24961 gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat
    25021 cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc
    25081 ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa
    25141 cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc
    25201 cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag
    25261 ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa
    25321 catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg
    25381 cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg
    25441 tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc
    25501 aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga
    25561 ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt
    25621 ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct
    25681 ggacgctgtt gtattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc
    25741 tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg
    25801 tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac
    25861 caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc
    25921 cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa
    25981 accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc
    26041 agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct
    26101 atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg
    26161 aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt
    26221 tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc
    26281 aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc
    26341 cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct
    26401 cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc
    26461 ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt
    26521 gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct
    26581 aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gcccgctgcc ggttctcggc
    26641 gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg
    26701 aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc
    26761 tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact
    26821 tccgcagacc gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa
    26881 atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc
    26941 gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc
    27001 tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga gcgtatcgag
    27061 aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc
    27121 acttctgagc gcagccgtcg tctgtctccg cctgcgttcc gttatcagct gactgaactt
    27181 gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg acgaaccgcg taccgttaaa
    27241 gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca
    27301 gagatcaacc gtgttgcagc gtctcagggt gtagaactgg gtgcagggat tgaaatcgtt
    27361 gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgaactgcg taagaacaaa
    27421 ggcatgaccg aaaccgttgc ccgcgaacag ttggaagaca acgtggtgct cggtacgctg
    27481 atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac
    27541 accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc
    27601 gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg
    27661 gatccgaccg ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc
    27721 ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt
    27781 agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg
    27841 atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa
    27901 gcgccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc
    27961 ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg
    28021 ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc
    28081 gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat
    28141 ccgcagcttt gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt
    28201 ctcttaattt gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt
    28261 gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg
    28321 tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt
    28381 atcttttttg ttgctatttt aaccagcctt gccacctggg tagttccggt ggggatgttt
    28441 gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat
    28501 ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag
    28561 ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga aggattaacc
    28621 tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc
    28681 gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc
    28741 catacccgcg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc
    28801 ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta
    28861 atggtccggc tgggctatga cagtattacc accgtcctgg tgacctatat tgccacgcaa
    28921 atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc
    28981 ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg
    29041 attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca
    29101 cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg ctgatgttga acaacgtccg
    29161 tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt
    29221 tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg
    29281 ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc
    29341 atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt
    29401 ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta
    29461 aataccatcc tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc
    29521 tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg ttctggtcag
    29581 gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgccag
    29641 gttaccgtgc tggcattcca gtttggtgat ggcttcagtc acatcattta cccaacctca
    29701 gcttcgttaa tggcaactct cggtgtatgc agggtggact tccgtaactg gctgaaggtg
    29761 ggcgcgaccc tacttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag
    29821 ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc
    29881 gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc
    29941 agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa
    30001 acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca gctgaacaac
    30061 gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg
    30121 acaccggcaa tgcccagctc ttcttccgct tcgcgacgcg cggattccag cagttgctca
    30181 tcggcctgaa ccacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc
    30241 tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca
    30301 cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc
    30361 tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg
    30421 aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta
    30481 atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta
    30541 tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga gccggggttg
    30601 aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc
    30661 acatcgttct ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa
    30721 aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc
    30781 agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt atgtgccact
    30841 tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg
    30901 ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg ggcaaacaac
    30961 tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc
    31021 atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca
    31081 gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa
    31141 tgtcgcataa tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat
    31201 cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag
    31261 tactaacaaa tgttctgcca tcgcaaacag tttctctgca ctggcttttc cctcggccgt
    31321 tagtggcgct tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg
    31381 catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc
    31441 aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat
    31501 ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg
    31561 accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat
    31621 atgaaacgac aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa
    31681 aaagtgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa
    31741 gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt
    31801 aattcgccgc gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg
    31861 atcgatctct atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa
    31921 gcagagcttg attatcgctt gattaaggta gacctgggga aaggcggtca gtttcgtccg
    31981 gaatttttgc gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc
    32041 gatggcggcg aaccgttaag cctctttgag tctggtgcca ttttgttgta tctggctgag
    32101 aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta
    32161 ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca
    32221 gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac
    32281 catgtactga acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt
    32341 gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat tgacctcgca
    32401 atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag
    32461 gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc
    32521 gtgtattatt tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta
    32581 ttattcgtat aaagaacctt cgtttacgta cgtttatcgg aattaaggaa gaagaaatta
    32641 acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca
    32701 ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc
    32761 atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg
    32821 cacgtgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc
    32881 gctacgccga ttcggtatcc atgaccttaa gctggcagcg ttaaccgcca tatcgggagg
    32941 ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg
    33001 tttgctggag ctgggccatc aaatcacggt agtgacgcgt aacccacaga aagccagttc
    33061 cgttctcggc cctcgggtga cgctatggca agggctagcc gatcaaagca acctcaacgg
    33121 cgttgatgcg gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacga
    33181 gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat
    33241 taatgccagc gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg
    33301 cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttacccataa
    33361 actctgcgcc cgctgggaag aaattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg
    33421 cctgctgcgt accggtgtag tgctggcacc ggatggcggt attctcggta aaatgctgcc
    33481 accgtttcgt cttggcctgg gcgggccgat tggttccggt cggcagtatc ttgcctggat
    33541 tcatatcgat gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc
    33601 atttaatatg gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca
    33661 tgcgctgcat cgcccggcca ttttgcgcgt ccccgcgacc gccattcgac tgttaatggg
    33721 cgaatcttca gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc
    33781 gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg
    33841 tggtttacag caaacatccg ccagttaact cccggtgtta caggattagt ggctttgcgc
    33901 gataagatcg tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct
    33961 atccagcatc tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc
    34021 ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttccgc
    34081 tatctcacca ataaatgcac gaaaacgctg ggcatcccct gtttgtaata cgtaattaaa
    34141 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    34201 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat
    34261 tttgcggtgt tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc
    34321 tgatgaatat attttgtggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga
    34381 ataaggataa ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc
    34441 tttatagccc gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa
    34501 agccattaat tcttccggct tactttggtg ctctatcact acataaatat aacccactcc
    34561 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cgtcatcaat
    34621 aaaactgttt ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg
    34681 aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    34741 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    34801 aagatgacgc gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg
    34861 acagcgaatt cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac
    34921 tcgcaaattc cccatgaaaa tactccggca gcgttagcca ccggagagga aattatttca
    34981 gcgatccctt aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg
    35041 gcgcgccctc ttcttctatt ttcccctgat ggaggaaaat gacatgagta gaaacatggc
    35101 gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct
    35161 gcataatacg caacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa
    35221 acagtaaaac ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac
    35281 cgccggaaag atgcaccgga tatttcccct gcgcacgttc gtctatcccg acttttgcca
    35341 gatacttgac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    35401 cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca
    35461 tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt
    35521 ctttgtcgcg caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt
    35581 tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga
    35641 tgcttattac atctccggca ttcgcttgca gtgagacccc tttcagcact tcatgttcgc
    35701 cgtagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    35761 gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc
    35821 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga
    35881 tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcaccgt ggccgtaaac
    35941 gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata
    36001 cgcagcgcag aaggcaaaat aatgcaacga tacattttaa aagtcgagaa gccataggct
    36061 ctggcggctt caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta
    36121 gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag
    36181 aaagcgttaa ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac
    36241 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    36301 aactggatgt atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg
    36361 cctatcacta ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa
    36421 ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    36481 tcttcacacc cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacagtgg
    36541 tgaaaaccag gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt
    36601 tgcctgccag ttgggtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg
    36661 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    36721 ccggaaacat gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct
    36781 ctatatgccc tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac
    36841 cgtagataaa accgagagta ataataccag cgaccatcgg atcgatatca atctgcccga
    36901 cgcccatcgc ctccgtcacc gtgtttagcg caatctgcaa accgtagaaa atcagcagca
    36961 tcaacaccaa gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg
    37021 aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    37081 ctacagaact gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    37141 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    37201 ccggttaccc gaagggagcg ggctttcttt caccgatggc gattaaccgc cataaacatc
    37261 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    37321 ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    37381 tacgccaaac agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac
    37441 gggttgtttg aggaaacctt cactggcagc gacctcatcc tggaacgcgg catcaatacg
    37501 tccggcagtc aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc
    37561 ttttggtgcc caatgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac
    37621 ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa
    37681 acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt
    37741 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    37801 atccagcgga ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc
    37861 cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata
    37921 ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca gcaaacgccg cagttgcgct
    37981 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    38041 cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc
    38101 atcaaggcaa aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta
    38161 tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca
    38221 tcaggtacag cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt
    38281 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag
    38341 ccgtcagttc agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt
    38401 tgtcttttac tgatgagcca gcaaaggcga aatctttacc agcaggttgc ttgaggaatc
    38461 cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg
    38521 aatagaccaa atcctggttg gcataggcca ccacatccac gcctttgcta cgccaggtct
    38581 cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    38641 aatccagcgt tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt
    38701 acagcttgtc ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa
    38761 taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa
    38821 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    38881 caacaaaatc acctttagca tctttcgacg agaacggcgc ataggtggta tcggttccga
    38941 tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaaca
    39001 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    39061 gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg
    39121 acaacagtaa cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt
    39181 gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg
    39241 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    39301 ccctgccagc gggcaaagag atcttcagga agggttatcg caaactggtc aagaacacga
    39361 ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg
    39421 ggaggcataa tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag
    39481 tgcaatggtg tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct
    39541 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggaaag ggttttgatt
    39601 gaacagggta aaatcaccat ccctagcgtc tggaaagaac cggaagagat gctggcggca
    39661 atatcgcgcg catcgtgcgt gacatcggct aatgcctgca cttcgcgcag agaaaaatcc
    39721 gtttcgaggg ataaggtctg gcgcgctgcc tggctcatca ccagatgcgt ttcgatatct
    39781 gtgacatcgc gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcgccgctg
    39841 atgcctacaa tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    39901 aggatttcgg cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca
    39961 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    40021 atcattacgt aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    40081 gacgccgttg aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc
    40141 gtcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    40201 ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata
    40261 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    40321 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac
    40381 cagcaggacg tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga
    40441 cttacgacgc agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac
    40501 gaagccctgg cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga
    40561 ggtttctggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    40621 ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    40681 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    40741 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc
    40801 gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat
    40861 atattctgta cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt
    40921 tggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catatgcgcc
    40981 gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta
    41041 gtggcggaag ttgtccagct cgctagcgaa gatattaagc agaatttccg agtcggaagt
    41101 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    41161 cagattgccg ttgtgggcaa gcgtaatgcc atacggagag ttaacgtaaa acggctgcgc
    41221 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    41281 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    41341 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    41401 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    41461 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    41521 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    41581 ctgcgggatc agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc
    41641 gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    41701 cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    41761 cacgatagca ccaacgatca gggtagcgat aaacagtacc gcgatggcaa ttccatttcg
    41821 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    41881 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    41941 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    42001 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    42061 aaacgtttgc gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt
    42121 caacgccatc gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta
    42181 aacgccttat ccgacctacg gttctaacac tgcccaggcc tgataagacg cgccagcgtc
    42241 gcatcaggca agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga
    42301 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac
    42361 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    42421 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag
    42481 ttgcacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc
    42541 tggcttcggc gcaggtggtg cttcaacctt cggtttaggc ggctccaccg gtttcggctt
    42601 cggtggggcg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggt
    42661 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    42721 cggctgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc
    42781 cgctttcggc accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc
    42841 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    42901 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    42961 cagtgtggaa agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg
    43021 catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    43081 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    43141 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    43201 gcacgcgccc gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg
    43261 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    43321 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg
    43381 cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat
    43441 tttccagcgt gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc
    43501 actcaacgcc ccgacgttgt aacagtgcac ctttttcctg tgccacatca gcaatggtag
    43561 aaggcatttc cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct
    43621 cgcgaccaat actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg
    43681 ttactaccgc gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    43741 cttccagaat caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac
    43801 cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg
    43861 cggtgtgggc cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca
    43921 ccagatgcgg tgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    43981 gcgtacggca ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg
    44041 gtttcagaac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    44101 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    44161 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    44221 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg
    44281 gtaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    44341 ccagaatgct cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatggcgc
    44401 ctttctcgat caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct
    44461 gttcgataac acgcggaccg gcaaagccaa ttaacgcttt cggttcagcg atgttgagat
    44521 cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga
    44581 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    44641 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg
    44701 ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc
    44761 ccattgaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    44821 gagtgccttt catcaccacc agcgcatctt tttcgccagt ttctttctgc gcagatgcca
    44881 gacggtcttt atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc
    44941 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    45001 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    45061 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    45121 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    45181 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    45241 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    45301 gaaccgcgga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa
    45361 ttcgaaaacc ccaggcaata ttgaaacaaa aataatgcca acaatcatca gcttaaggtt
    45421 gctctgaatg aaggggagcg tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca
    45481 caacagtgcg ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc
    45541 cgcaacaaac ggcgcgaacg ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt
    45601 gccgccatgt ctctcgtaaa actgatgagt cttatcaaga taactgcgac gaaagatttt
    45661 cgaatccggg ttactgaaca gtttgtcacc aaataaccgt ccaatggtat agttcactgc
    45721 atcaccgaca atagcggcaa tcaacataag caccaccatc atatggacat tgagatcgtt
    45781 agtttccagc gacgccaatg caccagcaac aaacagcagc gaatcaccag gcaggaatgg
    45841 cgttaccacc agccccgttt cgcagaacag aatcaaaaac agaatggcat aaacccagac
    45901 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    45961 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
    46021 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    46081 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
    46141 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    46201 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    46261 tgaccatatg atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac
    46321 gcgtgacatt aatatgcata acgttacgcc acggggttcg ggactggcac tgcaccgcac
    46381 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
    46441 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat
    46501 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    46561 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    46621 aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac
    46681 cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    46741 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    46801 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt
    46861 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    46921 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    46981 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac
    47041 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc
    47101 acgcgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
    47161 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
    47221 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa
    47281 caacggcagc atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat
    47341 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
    47401 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
    47461 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    47521 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    47581 gagagcaaac agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
    47641 ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
    47701 gaattcagcg gcatcctgca cggtgattgt cttaccacca aagcgcagtt gttcgcctgc
    47761 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    47821 ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc
    47881 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
    47941 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    48001 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
    48061 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    48121 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
    48181 gggccatgca gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg
    48241 tgctgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct
    48301 ttaccttcca gggtataacc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac
    48361 tccacgttaa gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
    48421 tcattcaggc aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc
    48481 agaatcgctc ccggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg
    48541 tacggcccat ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat
    48601 tcatccagcg agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc
    48661 aatgttttaa tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg
    48721 cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt
    48781 agtgaggaga aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa
    48841 ccaattcccg cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg
    48901 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    48961 accatcagcg catccgcgtc tgccagttga gcaacgggga ttggacgccc gggaaccgcg
    49021 gtcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    49081 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc
    49141 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    49201 ttccggccct cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg
    49261 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    49321 ctcactgacg caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    49381 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca
    49441 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    49501 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    49561 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    49621 ggaagggaag atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt
    49681 accggcggag cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga
    49741 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
    49801 aattccagcg aaactgttta accatctggt gacctggttg caggcgcgcc agacgttaag
    49861 ccagcaaaat acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc
    49921 cagtgaactg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
    49981 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
    50041 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac
    50101 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    50161 gctggtctgg ctggttcgtt agccccgacg aaatgacagt atcgtgacaa taattcccag
    50221 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    50281 tcccgccagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    50341 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
    50401 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcacctgg
    50461 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
    50521 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    50581 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    50641 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt
    50701 ccagataaac ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt
    50761 gcgcactgtg ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag
    50821 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc
    50881 accgtaaatc gccattccat tccatgacat cactttgcca gagtgttttg gccctacgat
    50941 gcctaacccc caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa
    51001 acgcccgatg accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag
    51061 cagcaacgcg ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
    51121 accatattga tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg
    51181 aatcccgacg gcaatcccga ccatggtatt gccatagccc agatcatgat gaacaaacag
    51241 cgggataacc ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat
    51301 gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
    51361 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
    51421 ttacagtgcg ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg
    51481 gaagtttgcc tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc
    51541 ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg
    51601 tcttatccga cctactccga ctactccgac ctactgcgaa ttaatctttc agcttgcgca
    51661 ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt agacataacg gtagtcagtt
    51721 cgcgatcggt cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc agctcttcaa
    51781 tgttgatgct cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct
    51841 gtacgccagc agcgcccaga gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc
    51901 tcttatcgcc gaacacttca cggatagctg ccagttcttt cacgtcgcca accggagtcg
    51961 aagtaccatg agagttcagg taatcgattg gggtatcaac gccgtgcatc gccatcttca
    52021 tgcagcgtac tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc
    52081 cgtagccaac gatttcagca tagatgtgag caccacgcgc cagcgcgtgt tccagctctt
    52141 caaccactac cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag
    52201 tacgggaggc tttttccggg gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga
    52261 attcgcaggc catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt
    52321 tgcccagttg gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg
    52381 agctgatgga gtagttaacg ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc
    52441 cggatgccat cgctttggtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc
    52501 gcattgcgtc agcgccgaac acctggaaac gcggggaacc gccgccagaa cctgcaatca
    52561 ggccaacgcg cgggttattc tggtaagctt ccggagaaag gcccgcatct gcgattgcct
    52621 gctccataga aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt
    52681 caatgaggcc agtggtatcc agttttacgt tgccccagac gtggctacgc atgccggaat
    52741 ccttcagctc ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt
    52801 cctgctggtt attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca
    52861 ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg
    52921 tacgccgaac aagtccgatc agccatttaa tagagaaatt tgcgcagcct tacacacatc
    52981 gctaagatcg agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa
    53041 cctgccaacc tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc
    53101 tatttttcca acgataacgg gctggaagag acgcgttatg tttttctggg aggcaaccaa
    53161 ttagaggtac gctttcctga gcatccacat cctctgtttg tggtagcaga gagcggcttc
    53221 ggcaccggat taaacttcct gacgctatgg caggcatttg atcagtttcg cgaagcgcat
    53281 ccgcaagcgc aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt
    53341 gcggatttag ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt
    53401 caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaggccgc
    53461 gtgacgctgg atttatggtt tggcgatatt aacgaactga ccagccaact ggacgattcg
    53521 ctaaatcaaa aagtagatgc ctggtttctg gacggctttg cgccagcgaa aaacccggat
    53581 atgtggacgc aaaatctgtt taacgccatg gcaaggttgg cgcgtccggg cggcacgctg
    53641 gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc aggacgccgg attcacgatg
    53701 caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca
    53761 ttaccgctcc cctgctccgc gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg
    53821 gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc tggcgctatt acggcgcggc
    53881 tggcaggtaa cgctttattg cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc
    53941 cagggggcgc tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct
    54001 aatgcgttta cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac
    54061 tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg
    54121 ttgtcaatgg atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa
    54181 attacgggcg ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc
    54241 ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat
    54301 tatcaatatc agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca
    54361 ggagatcagc aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccga
    54421 ttcagccaaa cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg
    54481 acaacgccgg aactggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca
    54541 caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat
    54601 acggcgtaca gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg
    54661 caggcacagt gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt
    54721 tgtgccaccc gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc
    54781 gtggaatatg cgtcgttggc ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac
    54841 gatctcttta tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc
    54901 gagattctgg cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg
    54961 gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg
    55021 gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga acgccttatc cgacctacgt
    55081 gtgac
//
LOCUS       DAZGCS010000018        54472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGCS010000018 DAZGCS010000000
VERSION     DAZGCS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.018"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..677
                     /gene="rpiB"
                     /locus_tag="ACHK01_001171"
                     /gene_synonym="alsI"
     CDS             228..677
                     /gene="rpiB"
                     /locus_tag="ACHK01_001171"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO8096152.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
     gene            746..1075
                     /gene="yjdP"
                     /locus_tag="ACHK01_001172"
     CDS             746..1075
                     /gene="yjdP"
                     /locus_tag="ACHK01_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO8096153.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1222..1980)
                     /gene="phnP"
                     /locus_tag="ACHK01_001173"
     CDS             complement(1222..1980)
                     /gene="phnP"
                     /locus_tag="ACHK01_001173"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO8096154.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(1982..2416)
                     /gene="phnO"
                     /locus_tag="ACHK01_001174"
     CDS             complement(1982..2416)
                     /gene="phnO"
                     /locus_tag="ACHK01_001174"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO8096155.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2403..2960)
                     /gene="phnN"
                     /locus_tag="ACHK01_001175"
     CDS             complement(2403..2960)
                     /gene="phnN"
                     /locus_tag="ACHK01_001175"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO8096156.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2960..4096)
                     /gene="phnM"
                     /locus_tag="ACHK01_001176"
     CDS             complement(2960..4096)
                     /gene="phnM"
                     /locus_tag="ACHK01_001176"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO8096157.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAL
                     CRVRQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(4093..4773)
                     /gene="phnL"
                     /locus_tag="ACHK01_001177"
     CDS             complement(4093..4773)
                     /gene="phnL"
                     /locus_tag="ACHK01_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO8096158.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4884..5642)
                     /gene="phnK"
                     /locus_tag="ACHK01_001178"
     CDS             complement(4884..5642)
                     /gene="phnK"
                     /locus_tag="ACHK01_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO8096159.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5639..6484)
                     /gene="phnJ"
                     /locus_tag="ACHK01_001179"
     CDS             complement(5639..6484)
                     /gene="phnJ"
                     /locus_tag="ACHK01_001179"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO8096160.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6477..7541)
                     /gene="phnI"
                     /locus_tag="ACHK01_001180"
     CDS             complement(6477..7541)
                     /gene="phnI"
                     /locus_tag="ACHK01_001180"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO8096161.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7541..8125)
                     /gene="phnH"
                     /locus_tag="ACHK01_001181"
     CDS             complement(7541..8125)
                     /gene="phnH"
                     /locus_tag="ACHK01_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO8096162.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(8122..8574)
                     /gene="phnG"
                     /locus_tag="ACHK01_001182"
     CDS             complement(8122..8574)
                     /gene="phnG"
                     /locus_tag="ACHK01_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO8096163.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8575..9300)
                     /gene="phnF"
                     /locus_tag="ACHK01_001183"
     CDS             complement(8575..9300)
                     /gene="phnF"
                     /locus_tag="ACHK01_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO8096164.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9321..10100)
                     /gene="phnE"
                     /locus_tag="ACHK01_001184"
     CDS             complement(9321..10100)
                     /gene="phnE"
                     /locus_tag="ACHK01_001184"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO8096165.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(10206..11222)
                     /gene="phnD"
                     /locus_tag="ACHK01_001185"
     CDS             complement(10206..11222)
                     /gene="phnD"
                     /locus_tag="ACHK01_001185"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8096166.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(11247..12035)
                     /gene="phnC"
                     /locus_tag="ACHK01_001186"
     CDS             complement(11247..12035)
                     /gene="phnC"
                     /locus_tag="ACHK01_001186"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO8096167.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(12168..12611)
                     /gene="yjdN"
                     /locus_tag="ACHK01_001187"
     CDS             complement(12168..12611)
                     /gene="yjdN"
                     /locus_tag="ACHK01_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO8096168.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12771..13106)
                     /gene="yjdM"
                     /locus_tag="ACHK01_001188"
     CDS             complement(12771..13106)
                     /gene="yjdM"
                     /locus_tag="ACHK01_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO8096169.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            13508..15736
                     /gene="crfC"
                     /locus_tag="ACHK01_001189"
     CDS             13508..15736
                     /gene="crfC"
                     /locus_tag="ACHK01_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO8096170.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            15733..16611
                     /gene="rdcB"
                     /locus_tag="ACHK01_001190"
     CDS             15733..16611
                     /gene="rdcB"
                     /locus_tag="ACHK01_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO8096171.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRKHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            16875..18377
                     /gene="proP"
                     /locus_tag="ACHK01_001191"
     CDS             16875..18377
                     /gene="proP"
                     /locus_tag="ACHK01_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO8096172.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            18489..18578
                     /gene="pmrR"
                     /locus_tag="ACHK01_001192"
     CDS             18489..18578
                     /gene="pmrR"
                     /locus_tag="ACHK01_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO8096173.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(18554..19654)
                     /gene="pmrB"
                     /locus_tag="ACHK01_001193"
     CDS             complement(18554..19654)
                     /gene="pmrB"
                     /locus_tag="ACHK01_001193"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO8096174.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19655..20323)
                     /gene="basR"
                     /locus_tag="ACHK01_001194"
     CDS             complement(19655..20323)
                     /gene="basR"
                     /locus_tag="ACHK01_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO8096175.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(20320..21963)
                     /gene="eptA"
                     /locus_tag="ACHK01_001195"
     CDS             complement(20320..21963)
                     /gene="eptA"
                     /locus_tag="ACHK01_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO8096176.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(22067..23404)
                     /gene="adiC"
                     /locus_tag="ACHK01_001196"
     CDS             complement(22067..23404)
                     /gene="adiC"
                     /locus_tag="ACHK01_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO8096177.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(23541..24302)
                     /gene="adiY"
                     /locus_tag="ACHK01_001197"
     CDS             complement(23541..24302)
                     /gene="adiY"
                     /locus_tag="ACHK01_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO8096178.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(24627..26894)
                     /gene="adiA"
                     /locus_tag="ACHK01_001198"
     CDS             complement(24627..26894)
                     /gene="adiA"
                     /locus_tag="ACHK01_001198"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO8096179.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(27093..28001)
                     /gene="melR"
                     /locus_tag="ACHK01_001199"
     CDS             complement(27093..28001)
                     /gene="melR"
                     /locus_tag="ACHK01_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO8096180.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRAHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            28284..29639
                     /gene="melA"
                     /locus_tag="ACHK01_001200"
     CDS             28284..29639
                     /gene="melA"
                     /locus_tag="ACHK01_001200"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO8096181.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            29742..31163
                     /gene="melB"
                     /locus_tag="ACHK01_001201"
     CDS             29742..31163
                     /gene="melB"
                     /locus_tag="ACHK01_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO8096182.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(31302..31931)
                     /gene="yjdF"
                     /locus_tag="ACHK01_001202"
     CDS             complement(31302..31931)
                     /gene="yjdF"
                     /locus_tag="ACHK01_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO8096183.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(32054..33700)
                     /gene="fumB"
                     /locus_tag="ACHK01_001203"
     CDS             complement(32054..33700)
                     /gene="fumB"
                     /locus_tag="ACHK01_001203"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8096184.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(33778..35118)
                     /gene="dcuB"
                     /locus_tag="ACHK01_001204"
     CDS             complement(33778..35118)
                     /gene="dcuB"
                     /locus_tag="ACHK01_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO8096185.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(35689..36408)
                     /gene="dcuR"
                     /locus_tag="ACHK01_001205"
     CDS             complement(35689..36408)
                     /gene="dcuR"
                     /locus_tag="ACHK01_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO8096186.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(36405..38036)
                     /gene="dcuS"
                     /locus_tag="ACHK01_001206"
     CDS             complement(36405..38036)
                     /gene="dcuS"
                     /locus_tag="ACHK01_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO8096187.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            38217..38447
                     /gene="yjdI"
                     /locus_tag="ACHK01_001207"
     CDS             38217..38447
                     /gene="yjdI"
                     /locus_tag="ACHK01_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO8096188.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            38459..38731
                     /gene="yjdJ"
                     /locus_tag="ACHK01_001208"
     CDS             38459..38731
                     /gene="yjdJ"
                     /locus_tag="ACHK01_001208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8096189.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            38958..39254
                     /gene="ghoS"
                     /locus_tag="ACHK01_001209"
     CDS             38958..39254
                     /gene="ghoS"
                     /locus_tag="ACHK01_001209"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8096190.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            39282..39455
                     /gene="ghoT"
                     /locus_tag="ACHK01_001210"
     CDS             39282..39455
                     /gene="ghoT"
                     /locus_tag="ACHK01_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO8096191.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(39574..41091)
                     /gene="lysS"
                     /locus_tag="ACHK01_001211"
     CDS             complement(39574..41091)
                     /gene="lysS"
                     /locus_tag="ACHK01_001211"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8096192.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(41328..42785)
                     /gene="dtpC"
                     /locus_tag="ACHK01_001212"
     CDS             complement(41328..42785)
                     /gene="dtpC"
                     /locus_tag="ACHK01_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO8096193.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(42844..44991)
                     /gene="cadA"
                     /locus_tag="ACHK01_001213"
     CDS             complement(42844..44991)
                     /gene="cadA"
                     /locus_tag="ACHK01_001213"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO8096194.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(45071..46405)
                     /gene="cadB"
                     /locus_tag="ACHK01_001214"
     CDS             complement(45071..46405)
                     /gene="cadB"
                     /locus_tag="ACHK01_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO8096195.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(46771..48309)
                     /gene="cadC"
                     /locus_tag="ACHK01_001215"
     CDS             complement(46771..48309)
                     /gene="cadC"
                     /locus_tag="ACHK01_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO8096196.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(48569..48683)
                     /locus_tag="ACHK01_001216"
                     /pseudo
     CDS             complement(48569..48683)
                     /locus_tag="ACHK01_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(49058..49630)
                     /locus_tag="ACHK01_001217"
     CDS             complement(49058..49630)
                     /locus_tag="ACHK01_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8096197.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     GSAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(49727..49924)
                     /locus_tag="ACHK01_001218"
     CDS             complement(49727..49924)
                     /locus_tag="ACHK01_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8096198.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(49936..50424)
                     /locus_tag="ACHK01_001219"
     CDS             complement(49936..50424)
                     /locus_tag="ACHK01_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8096199.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTRDVGESEWIHFT
                     GTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDANAECLPGLPTF
                     DW"
     gene            complement(50421..50798)
                     /locus_tag="ACHK01_001220"
     CDS             complement(50421..50798)
                     /locus_tag="ACHK01_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8096200.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(50888..51256)
                     /locus_tag="ACHK01_001221"
     CDS             complement(50888..51256)
                     /locus_tag="ACHK01_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8096201.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(51419..51640)
                     /locus_tag="ACHK01_001222"
     CDS             complement(51419..51640)
                     /locus_tag="ACHK01_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8096202.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(51703..52179)
                     /locus_tag="ACHK01_001223"
     CDS             complement(51703..52179)
                     /locus_tag="ACHK01_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO8096203.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(52195..52680)
                     /locus_tag="ACHK01_001224"
     CDS             complement(52195..52680)
                     /locus_tag="ACHK01_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8096204.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFIAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTEYYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(52735..53553)
                     /locus_tag="ACHK01_001225"
     CDS             complement(52735..53553)
                     /locus_tag="ACHK01_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8096205.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(53653..53886)
                     /locus_tag="ACHK01_001226"
     CDS             complement(53653..53886)
                     /locus_tag="ACHK01_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8096206.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(53965..54420)
                     /locus_tag="ACHK01_001227"
     CDS             complement(53965..54420)
                     /locus_tag="ACHK01_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO8096207.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT        12980 a        13574 c        14405 g        13513 t
ORIGIN      
        1 aattttattt catttaatta gtttaaaaac atgatgttat ttgtttttct ggttttttag
       61 atgatagatg ttgctggatg gtgagtgtct ttaacgctat tgcgctgtca gatgtaactc
      121 tgtaaaacag atcaggaagg cgtaattcat cgattttggg tgtttttaat tcattaacat
      181 cacaaatgtt ttttgattgt gaagttttgc acggacgggg aagatgaatg aaaaagattg
      241 catttggctg tgatcatgtc ggtttcattt taaaacatga aatagtggca catttagttg
      301 agcgtggcgt tgaagtgatt gataaaggaa cctggtcgtc agagcgtact gattatccac
      361 attacgccag tcaagtcgca ctggctgttg ctggcggaga ggttgatggc gggattttga
      421 tttgtggtac tggcgtcggt atttcgatag cggcgaacaa gtttgccgga attcgcgcgg
      481 tcgtctgtag cgaaccttat tccgcgcaac tttcgcggca gcataacgac accaacgtgc
      541 tggcttttgg ttcacgagtg gttggcctcg aactggcaaa aatgattgtg gatgcgtggc
      601 tgggcgcaca gtacgaaggc ggtcgtcatc aacaacgcgt ggaggcgatt acggcaatag
      661 agcagtggag aaattgagat tcatccacta cttgcatgga tgagtaatga ctaatgtgga
      721 tagagtttct ttttgaggtt ggctaatgaa acgctttccg ctatttcttt tattcaccct
      781 gctcacgctg tccaccgttc cggcccaggc cgatattatc gacgacacca ttggcaatat
      841 tcagcaggcg attaacgacg cttataaccc tgaccgtggg cgagactatg aagattcgcg
      901 cgacgacggc tggcagcgtg aagtgagcga cgatcggcgc agacaatatg acgaccgccg
      961 ccgccagttt gaagatcgcc gcaggcagct ggacgatcgc cagcgccagc ttgatcagga
     1021 gcgtcggcag ctagaagatg aagagcggag aatggaagat gagtatgggc gatgaggttg
     1081 ggtatggagc aggcatcgcc cctcacccca atggggagag ggagaaaacc agcgcaatat
     1141 tcaatatcga gcacaatcgg tcccctcgcc cctctgggga gagggttagg gtgagggggc
     1201 cagcgcgcac attccctctc atcacgccac cccaatctcc atcccatcaa accccgcctc
     1261 aaaacccggc ggcagcgcat tctccatcag ccacgcatca aactggtggc tgatatgggt
     1321 cagaatcacc cgtggcgagt ggataacctg attgagcgca agcacggtat ttaaatcaca
     1381 gtgattacgc ggcgcatcct cgcgcggcgg gtggctgcaa tcaatcacca tcacctgcgg
     1441 atgattattg agtaaaaatt tctgtgtttt ttccggcagg ccagcggtgt cagatagcca
     1501 cgccacccgg ctatgtgccg tttccagcag ataaccgaag gtcagttttg agtggttaag
     1561 cggcagcggc gtgacctgca acccctgcaa atcaaatacc acaaacggtt ccactgtgtg
     1621 gctgaaatca agcaggcccg gatgtttaaa cagatcgtcg caaccctggt catccggcgg
     1681 gccgtaaacc gggatcggat cgccaacgcc ccagcgcagc ggaaacagcc cctggacgtg
     1741 atccatatga taatgcgtca gcaaaaactg ctggaacgat ccgggcgacc agcgatcggc
     1801 gagatcgtgc agcccggcgt cgatcagggt gattgcgtcg ttaaacttca ctacgccgct
     1861 gcatggctgg cggcgatact gcggtgagcg ccgcgctcgg tcgcaggccg cgcactcgca
     1921 gccccatgcc ggaacgccct gtgcgccgcc ggtgccggtg agcgtgaggg tcagactcat
     1981 gttacagcgc cttggtgaag cggaagtgac tctgctcgta gccttctcgc agatagaaac
     2041 ggtgcgcgtc gtggcgcttc acgttggtcg aaagttcggt catttcggcc ccggcctggc
     2101 gggcttcttc ttctgcccac gccagtaact tactgccgac gttcagaccg cgcgcctgcg
     2161 gcattaccac caactcctga atttcgccga tccagttgac atgatgcaga tgaaactgca
     2221 aatgcaggcc gatcataccg acaacttcgc catccagcag cgccagatgg tagcgcatgt
     2281 tcgggtcgcg caggttggcg ttaaagccca cgcgaaacgc gtggtggtca aactccgcct
     2341 gttttagctc gcaaatcaac gcgtaaaccg cgtcggtgtc gtactgcgtg gccgggcgaa
     2401 gctcacaagc aggcatggtg tttctccttc tgatggatta gcgtcagcag cgtgtcgacc
     2461 gactggcgca ggctgccgtc attattgagc gtatggcaat cctgtggagt atagcgggcg
     2521 gcgcgcgcca gccgggcgtt aatttcactg gcgttttcgc ggccacggtt ttccaggcgc
     2581 tggcgcagga tctctggcga aacctgtaag caaatgggca gcagcgccga ttgatagcgc
     2641 gcccgcgcct gcggcagatg ggcgcgtgag ccgttgacca gcacgtcgaa tccagcgtgc
     2701 agccagaggt caatctcgac gccgacgcca taatacagac cgttagcgtg ccagcttaag
     2761 gccagcagat tttgccccgc gcgggtaaaa aactcctgct cgctcagggc gatatggttt
     2821 tcgcttccag cgctggcgtc gcgcgtgatg tagcgatgcg ccaccagtag ctgagtttgt
     2881 tcccgcaggc ggagttccgc cagcaggcta tctttcccgg agccggacgg ccccattaac
     2941 caaatcagtt ttcccatcat cagaacaccc ttttaccctg acgccagacg tggtcgatat
     3001 gaatgtggtt gcccttgcga tgcgccagca ccaggtcggc gcgtttaccc tcgccaatca
     3061 ccccgcgatc ctggagatta agcgcttgcg ccggattttt ggtcaccagc ttcaccgcct
     3121 gcggcagcgt aaagcggttg ctctcgtcat cggcgacgcg gaacgccgca tccagcaggc
     3181 tggcggggta gtagtcggaa gagaggatat ccagcaggcc aagctgcgcc agttcactgg
     3241 ccgccacgtt gccggagtgc gaaccgccac gcacgatatt tggcgcgccc atcagcacgt
     3301 tcatgccatg cttgcgcgag gcttccgctg cttcgaacgt agtgggaaat tcggcgatca
     3361 cgctgccaag ctggtgggat tcggcaacgt gggcgtgcgt ggcgtcatcg tggctggcga
     3421 gggcaatttg tcttacgcgg cacagggcgg cgatagtttc gcgattcggc tgcgaccagc
     3481 gtgcggcgag cgccagttgc tcttcttcgt actgctgcat ctgcgcatcg gtgagcgagt
     3541 atttgccctg ataatactcg cgatacttct cgcggttggc gaactggcgc tggcccggcg
     3601 agtgatccat cagcgacacc agcgtcaccg gctcgcgctg caccagtttt tcaaacagcg
     3661 gcagcgtagt gtgatgcggc agttcgcagc gcagatgcag gcggtgctcg gcgcggttga
     3721 cgccgcgttt ctgcgtctct tcgatggcgt tgatcatctt ctccagattt tccagccgat
     3781 cgccgccgtc gcgcacgtcg ccaattgcta cggcatccag tacggtggtg atgccgctcg
     3841 ccaccatcag cgcgtcgtgg ctgctcatcg ccgaatgggc gggccagtcg actttcgggc
     3901 gcggggtgaa gaatttatcg aggttgtcgg tatgcagctc aatcaggccg ggtagtagcc
     3961 agccaccttc gccgtccatc gcttctggca ggcggctctg gctttcggca aaggcgcgga
     4021 tttcgccatc ctgcacctcc agcgaaccgt gcaccacctc gttttccagc accagcttaa
     4081 cgttattgat aatcatgaag aggctcccat tgggtgcagg cggtcggcga cgtcattacg
     4141 tacagcttcg tcatggaaga tgcctacgat ggctgcgcca cgggttttgg cttcgcgaat
     4201 cagttccacc accgcggcgc tgtttttggc gtccagcgag gcggtaggtt cgtcaagcag
     4261 cagaatgggg tagtcgacga taaagccgcg ggcgatgttg acgcgctgct gttcgccacc
     4321 ggaaaatgtc gatggtgcca ggtgccacag gcgttccggc acgttcaggc gggtgagaag
     4381 acgcgcggct ttagcggcgc aggcttcacg cggaacgccg gtatcgagca gcggctgcat
     4441 caccacttcc agtgctgaga tacgcgggat gacgcgcaga aactggctca cccagccgac
     4501 ggtggttttg cggatttcca ccactttgcg cgctggcgcg gtgaccaggt ctacccactc
     4561 gtcaccgtgt ttgatctgga tttgaccttc gtcgggtaga tagttggcgt acagcgagcg
     4621 tagcagagtt gatttgccgc tgccggaatg gccgtggagc accacgcatt cgcccgcgtt
     4681 gacggtgagc gaggcgcgat tgaggacggg caggcgcacg ccgttttgct ggtgcaggat
     4741 gaaggtttta ctgacgtttt gtacgttaat catttttcgc ctcgtggttc gggtgtcgga
     4801 tgcgacgctg acgcgtctta tccgacctac ggggagcgca tttgtaggcc ggataaggcg
     4861 tttacgccgc atccggcacc ggctcaattc tgcaaaaccg atgacaccag cagctgtgta
     4921 tacggatgat gcgggtcgtc gagcacgcgg tcggttaacc cactctccac cacttgcccc
     4981 tgcttcatca ccagcaaacg gtccgccagc aggcgggcga cgcctaaatc atgggtgaca
     5041 atcaccaccg cgaggttcag ctccaccacc aggccgcgca gcaggtcgag caggcgggcc
     5101 tgcaccgaca catccagccc gccggtcggt tcatccataa acaccagctt cggatgcgtc
     5161 accaggttgc gggcaatctg caaacgctgc tgcataccgc cggaaaaggt ggtcggcagg
     5221 tcgtcgatcc ggttggcggg aatctccacc tcttccagcc acttctgcgc ggtggcgcga
     5281 atgtcgccgt aatgacgcgc cccggtcgcc atcaaccgct cgccgatatt gccgcctgcc
     5341 gagacctgac ggcgcaggcc gtcgagtgga tgctgatgca ctacgcccca ttcggtgcgc
     5401 agcaggcgac ggcggtcggc ctcgctcatt gcatacagcg aacggttctc gtagtgaatc
     5461 tccccctgct gcggcgtcag acgcgcggag atcgacttca gcagcgtggt cttcccggag
     5521 ccggattctc cgacaatgcc cagcacttct cccggccata aatcaaaaga gacatcgcta
     5581 aagcctttgc ccggcgcgta aaggtgggtc aggttattga ccgaaagtaa cggtttattc
     5641 attggctttt cgcctcgctc tgttggcggc aataatcggt atcggagcag acaaacatgc
     5701 ggtttccggc gtcatccagc accacttcat caagatagct gtgggtcgat ccgcagatgg
     5761 cgcatggctc atcccactgc tgaacggtga acgggtgatc gtcgaaatcg agactttcca
     5821 cgcgggtaaa cggcggcacc gcatagatgc gcttctcgcg ccctgctccg aacagctgca
     5881 gggcgggcat catgtccatt ttcgggttat cgaatttcgg gatcggcgac gggtccatca
     5941 cgtagcgccc gttcaccttc accggataag cataggtggt ggcgatatga ccgaagcggg
     6001 cgatatcttc atacagtttc acctgcatca cgccgtactc ctccagcgcg tgcatagtgc
     6061 gggtctccgt ttcgcgcggc tcgataaagc gcagcggttc cgggattggc acctggaaga
     6121 taatgatctg atcttcggtc agcggcgttt cggggatgcg gtgacgcgtc tggataagcg
     6181 tcgcatcgtc cgtacgttca gtggtgttta ccccggtcac gcgcttaaag aagttgcgaa
     6241 tcgacacggc gttggtggtg tcatccgcac cctggtcaat caccttcaac acgtcgcttt
     6301 cgccaatcac gctggcggtg agctgaatgc cgccggttcc ccagccgtag ggcatcggca
     6361 tctcgcgccc gccaaacggc acctgataac cggggatagc caccgctttt aagatggcgc
     6421 ggcggatcat gcgtttggtc tgctcgtcga ggtaggcaaa gttgtagccg ctcagattag
     6481 ccatggttct gctcctgttg cagacgtttg agtagctcca gttcggcctg gaaatcgacg
     6541 tagtgcggga gtttgaggtg cgagacaaag cctgcggctt cgacgttgtc ggcatgcgcc
     6601 agcacgaact cttcatcctg cgccgggcct gtcgcgtgct cgccgtattc cggggcttgc
     6661 aacgcacggt caaccagcgc cattgccatc gctttgcgct cgctcatgcc gaataccagc
     6721 ccgtagccgc gcgtgaagtg cggcggctca tccggcgggt cgataaaacc gttgaccatt
     6781 tcacattcgg tcatcagcag ttcgccgacg ttcaccgcaa atcccagctc ttccggcaca
     6841 atcgacacgt cgatgtaacc actgcggatc tcgcccgcga acgggtgatt gcgcccgtaa
     6901 ccgcgttggg tggagtaggc cagcgccagc aaatagcctt cgtcgccgcg catcaactgc
     6961 tgcaaacggg aagaacgtga gcaggggtaa accggcggcg tgcgggtgat atcatccggc
     7021 tgtgcgccgc tatcctcttc aaacttcgcc agcccctgac gcgccagcag gctgaaaacg
     7081 tgcggcgacg gctgttgttc gctgtcggcg gtggtcagcg tcggcgcttc accgtttgcc
     7141 agcagggtga aatcgagcag gcgatgggtg tagtcgtagg ttgggccaag cagctggccg
     7201 ccgggaatgt ctttataaac cgccgaaata cgccgttcga gacgcatctc tgtggtgtcg
     7261 agcggctcgc ttaccgcgag cttcgccagc gtggtgcggt aggcgcgcag caggaaaatt
     7321 gcttcgacgt tatcgccgct ggcctgtttc agcgccagcg ccgccagttc gcggtcggca
     7381 atgccgcctt cggtcatcac gcgatccacc gcaagattaa gctgctgttc aatctgggcg
     7441 acgctcagtt caggcaaatc ggtatcgccc cggcgtcggc tctcttgcag ggcgtgagcg
     7501 gcgtcgatcg ccttctcgcc ccctttcacg gcaacgtaca tcagcacacc tccacatgag
     7561 tggttcgcgg aatagccagc aggcgctcgc cgcaggtcag gatcaggtcg atgccgagcg
     7621 ggaacgggtg cgggcgctcg gtgagttcgt gcagaatgca ctccggcagc tgcggagcga
     7681 tcattcgttc ttcggcaatg cccgcgccgg tgagacgtaa catgcgcccg ccgctcaggc
     7741 tggcgacctg taaaatcagc gttgcacccg cttccggcgc aacggcggtg ccggtggaaa
     7801 gggcgttaag ctgttcgctg gaaatcgcct catccgtcac cgcgaaggtc gcctgttccg
     7861 gctggctgac cagcggcgcg ttggtatgaa aacgcaggct ctggctgacg atatcgttac
     7921 tcaatggcgc ggcaagccac accggcgtgt cgttatcggc cagcgtcagc agcacgctgg
     7981 tggtggcgat attcagcggc tgccagccgc gcttgagctg atgcagggcg acaatcacgc
     8041 ccggctcgct catggccttt aacaggcgac gaaaactgtg ctgggcatcc tgcacgggaa
     8101 gcataaaagc ggtttccagg gtcatgcgtt gtctccgcga accatcgtaa agaagtcgac
     8161 ccggctggcg ttcacttcgg cctggcgtgc ggcaatgcgt gccatacggt cagcgtccag
     8221 cggggcaata agggtttctg ataagttttg aaaatagcga gattgctgca tcagcgcgtc
     8281 aatcagcgcg cagcgttcgg cgtgctgttt gtcgcgcccc agcacccagc tgtagccgag
     8341 cgtgccgtca gtcaggcgta cggcggcgcg ggtgagcgtg gcgtcgccgg caaaaaagcg
     8401 ttcgccggtg ccgcccatcc gcgcctgaat ctgcaccagg ccggtttcgg cggcgcggat
     8461 aacctcatag tcggcggtga tgttcagtgc tttcaggcgg gctgccagtt cagccggttg
     8521 gctgtgcgcc agtacggaca tccagtgctg gcgggtcgcg gtatctgcgt gcattcagtg
     8581 ctccatagtg aattcaatca tgtcggcgcg cgtcaggctg acggagtact ccgccgggct
     8641 gctttcaccg tcacggtggt taagggtgcg cacgcacagc agcggcgaca tattcggaat
     8701 ttcaagacgc tggcactctt tggcctgggc gcgacgggcg ctgatccgcg tctggctgcg
     8761 gcgcagcgca attccggttt gctcgcgcag aaaatcgtgc agcgaaccgc tgtcgaagcg
     8821 ttgcagcgtc ggccaaaggg tgaggtccgc aaagtagtgg tcgattaaac agagcgcgac
     8881 gccgttgacg cgacgcaggg tgcgcaaatg gataacgttc tcgccctcgg taatccccag
     8941 cgcgtcagcg acgtgaccgg acgcggggcg caataccgac agcagttttt cgctggtggg
     9001 atggctaccc tggtccagca ggttctggct aaaacgcgcc tgggcgttga gcgggtaatc
     9061 gaacgggcgc atcagcacca gcacgccgac gccctgacgg cgctgtaccc agcctttttc
     9121 caccagttgg tcgatggcgc ggcgcagggt gtggcggttc acctcaaagc gcgccgccag
     9181 ttgctgctcg gcgggaagat agtcgccgca gcggtagtgt tgacgaagct cctgctcaag
     9241 ttttgcggct atctcctgat agcgtgttgg gtagctggtc ggatgtgtag acaagtgcat
     9301 agatatcaat gcctcgctta tcagataaag tgcttacgca accgttgaga gaggaaatcc
     9361 agcaggctga ccgtgacgat gataagcacc atcagggcgc aggtttgttg gaactggaaa
     9421 ccgcgaatcg cttcccacag ggtgacgccg atcccgcctg caccgaccat gccgacgacg
     9481 gtcgccgagc ggacgttgga ttcgaagcga tagagggagt aggagatcag cagcggcatc
     9541 acctgcggca gcacgccgta gaggatctct tcgagcttgt tggcgccggt ggcgcgaatg
     9601 ccttccaccg ggccgggttc aatcgcttcc accgcttcgg aaagcagttt ggagagcacg
     9661 ccggtggtgt ggataaacag cgccagcacg ccagcgaacg ggccgaggcc gacggcgacc
     9721 acgaacagca tggcgaagac catttcgtta atggcgcggc aggcgtccat caggcggcga
     9781 acgggctggt aaacccacca cggcaccagg ttttcggcgc tcatcaggcc gaaggggatg
     9841 gagagaacca ccgccagcgc cgtgccccag acggcgattt gcagcgtgac ggccatttcg
     9901 gtgaggtaat cctgccactg gctgaaatcg ggcgggaaga agtcggcggc gaacgtcgcc
     9961 atgttgccgc cgtctttaat cagcgtgagc ggggccattt ccgcgccctg ccacgagacg
    10021 accaacacgg cgagaacaac ggcccagctc agaagcgaga accagctgcg cttgggtggg
    10081 gcgatggtga tggtttgcat gtttggctcc ggtctgtagg ccggataagg tgcttgcacc
    10141 gcatccggca tcaacgcctg cacattgtcg gatgcgacgc cggggcgtct tatccgacct
    10201 acgatttact gcaccgcttt actcaccgaa ctcatcgcgc ttaacgcgtt gttcaggcgg
    10261 tccaggtcat ccagctgcgc ctgaatctcg gtggtttttg ccagcttgtc ctgctcattc
    10321 agtcctttat tgcttttcac gccctgcatc tctttaaaca gcgcgagctg gcgaatcggc
    10381 accagttgca ggtcgctgga agcgcggaac ggcgcccagc ccaggcgttc cagcaccgct
    10441 ttttcttccg gcgttttgcc gtagttcata aagaagtcgt agatcttgtc tttggtggtt
    10501 tcggaaaggt ttttacgcca gacgatcgga tcgcctggga tcagcggcga cttccagatc
    10561 accttcagtt ctttcagctt ctctggcgcg gaggttttca gcttgtcgag gttttcggtg
    10621 ttgttggtgg caacatccac ctgcttgttg gcgacggcca gcgcgttggt ttcatgccca
    10681 gcgttgacgg tgcgcttaaa gtcgctggcg gagatattgt ttttggcaaa gacgtagtaa
    10741 ccggggacga ggaagccaga ggtggagtta ggatcgccat tgccgaaggt gagatctttc
    10801 cgcttcgcca gcagatcgtt caggttgttg atcggactat ctttgttgac gatcaacacg
    10861 ctccagtaac ctggcgatcc atccgccgcg accgtctggg cgaagacctg gccgttggcg
    10921 cgatccaccg cttccattgc cgacaggttg ccgtaccagg cgatatccac tttattgaag
    10981 cgcattccct ggataatgcc tgcgtagtct ggggcaaaga aggcgttcac cttcacgccc
    11041 agcttcttct ccatatcctg taagaatggc gtccattgcg gtttcaggtt ttgctgtgat
    11101 tccgttgaaa taatgccgaa attcagcgcc ttttcctgct cttcggcgtg tgccgggctt
    11161 aacagggtgc tgaggctgaa catgctggtg aaggccagcg aggcaattat cttagcgttc
    11221 attcgttttc ctcaatgatg gggatgtcag gcagctttcg cgttctcttc gatgcgatta
    11281 atgctgcggt agagatggtc aaaacgttcg ttatcaaact gttggctgct gccgtcgtag
    11341 aaaacgtgcc cctggcgcag ggcgacgatg cgttcgcagt agcgcagggc gtaatccacc
    11401 tgatgcagcg tgacgaccac ggtgatgccg tcgttctgat tgatgtcgcg cagggtgtcc
    11461 atcacgatgc gggcggattc cgggtccagc gaggcgatgg gttcatcggc cagaatcacc
    11521 ttcgcctgct gcatcagcgc gcgggcaatc gccacacgct gctgctgtcc gccggagagg
    11581 gtggaaacgc gctgatgggc aaaatgcacc atgccaacgc gggtcagcgc ctgtaacgcg
    11641 cgctgtttct gctcgccggt gaaccagcta aaacaggtgc gccagaacgg cgtgctgccg
    11701 agcgcgccaa tcagcacgtt ctccagtacg ctcaggcggt tcaccaggtt gaattgttgg
    11761 aagatgtagc cggtgttggc gcggcttttg cggatatcgc gcgccagacg gccttcgcgc
    11821 tggactgtgc ggcccagcag ctcgatatgg ctgccggcgg atttatcgcc ggtaatcaaa
    11881 ccgcttaagt gacgtaaaag ggtggatttg ccggaacccg acggcccaag cagagccacc
    11941 atttcaccgt gatgaatgtt cagatcaacc gcatgcagcg cctgatgctg attgaaggtt
    12001 ttggcgagct tctcgacacg gataatcgtt tgcatgatgc agcctcccta aaaaagtggc
    12061 tccatcgtgg cggattattg tgacgatttg gttaattaaa agtgactaac agatgaagag
    12121 ttaacgggaa ttcgatgaca gtgcgggagg gcgggcctcc cggcgggtta ttgcgttggt
    12181 tgttgtttga cgacattaat catccacggt acgccaaatt tatcggtgac tttgccaaag
    12241 ccatgcgccc agaaagtttc ctgccaggcc atttcgattt ttccgttagc ggcaagattg
    12301 tcaaaccagc gttttccttc ttcgacctgt tgcgaatcga gcaccagcgt aaagccggag
    12361 tagctggctt ttcctgacgg tatagcatcg ctcatcatga tgtcgcttcc ggcaatgcgc
    12421 acgttggcgt gggcgatggc ggtatcggga aattgcattc cggaagggca gttctcggcg
    12481 ctgtcctgcg ctgattttgg catttcgccg aagctgattt tatagagcag ttcagcgccc
    12541 aacgtacgtt gataataagc aattgcgtcg gcacagttac cggcaaaaga gaggtaggga
    12601 cttaacggca tgatggtgac ctcagttaag agaagccagt taagtgtagt tcgaaattta
    12661 tacagatgag agagaagcct gataagacgc ggtcagcgtc gcatcaggca gtcggcatcg
    12721 ttgccggatg cggcgcgagc gccttatccg gcttaccgat cacaatacaa tcagttcttt
    12781 ttcacaaact cagatttcag tttcatcgga ccaaaaccgt cgattttgca atcgatgtta
    12841 tggtcgcctt caaccaggcg gatgtttttc actttggtgc caattttcag catcgaagag
    12901 ctacctttca ccttcagatc tttaatgatg gtaacgctgt cgccgtcagc cagcagattg
    12961 ccgttagcat ctttaacgat cagctcgtcg ctttcctgtg caggttctgc gtcgttccat
    13021 tcgtaggcac attccgggca gatgtacatg ccgttatctt cgtaagtgta ttcggagttg
    13081 cattttgggc agtgtggtaa tgacatgtgg atttcctcaa aagtcagcag ggcaaaagcg
    13141 ccaaaaaacg gcagattgcc gaaaaaggcc gcaattatac acaaaatccc tgagtttgtc
    13201 gggggtactg ctgattttag acgacgatcg ttttcatttg aaaggaaacg caaaaagaaa
    13261 ataatctttt tgcctctatt tattatttaa gataatgtta atgctctact tgttcttttt
    13321 tttattcaca tgatattttt atctttctga cgttttaaac gtatcagaag acgttattta
    13381 ttctgataaa aaacaacaca ctaaatataa aattatttct tgcagtaatt ataaaagccg
    13441 aaaacagaac tcaaaaacaa tctggctaaa taaaagtaac aaaatttgct ttaaggaaga
    13501 attttctatg tacacacaga ccctgtatga gttaagtcag gaggctgaac gcctgttaca
    13561 gctttctcgc caacagttgc agttactgga aaaaatgcct ctctctgtac ccggagacga
    13621 cgcgccacaa ctggctttac cctggagtca gcctaatatc gccgaacgtc acgcgatgct
    13681 gaataatgag ttgcgtaaaa tttcccgact ggaaatggtg ctggcaattg tcggtaccat
    13741 gaaagcaggg aaatcaacca ccattaatgc cattgttggt acggaggttc tgcctaatcg
    13801 taatcgccca atgactgcgc tgccgacgct tattcgccat acgcccgggc aaaaggaacc
    13861 ggtactgcat ttttcacatg tcgcgccaat cgattgttta attcaacaat tacaacagcg
    13921 cctgcgtgat tgcgatatta agcatctgac cgatgtgctg gaaatagata aagatatgcg
    13981 tgcgcttatg cagcggatcg aaaatggcgt cgctttcgaa aaatattatc tgggtgccca
    14041 gcctattttt cattgtctga aaagtttgaa tgatttagtg cgactggcga aggcgctgga
    14101 cgtcgatttt cctttttctg cttacgccgc cattgagcat attcccgtga ttgaagtgga
    14161 gtttgtccat ctggcggggc tggagagtta tcccggtcag ttgacgttac tggatacccc
    14221 cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg cttaaccagc agctggcacg
    14281 cgcctcggcg gtactggcgg tgctggatta tacgcaactg aaatcgatct ccgatgaaga
    14341 ggtccgtgag gcgattttgg cggtggggca atcggtgccg ctgtatgtgc tggtcaataa
    14401 gttcgatcaa caggatcgta acagtgacga cgccgaccag gtgcgggcac tgatttccgg
    14461 gacgctgatg aaaggctgta ttacgccaca gcagatattt ccggtgtcgt cgatgtgggg
    14521 ctacctggcg aatcgggcgc gccatgagtt agccaacaac ggtaagttac cagcgccaga
    14581 gcaacaacgc tgggtggaag attttgccca tgccgcgctc ggcaggcgct ggcgtcatgc
    14641 cgatctggcg gacctcgaac atattcgtca tgctgccgat cagttgtggg aagattcgct
    14701 gttcgcccag ccaattcagg cgttgcttca tgccgcttac gctaacgcct cgttgtatgc
    14761 tttgcgatct gctgcgcata aactgttgaa ttacgcgcag caggcgcggg aatacctgga
    14821 ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg cggcaaaata tccatcaggt
    14881 cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag gtgagcggcg agattaaaca
    14941 tgaaatcgag ctggccctga cctccgccaa ccactttctg cgtcaacaac aagatgcgct
    15001 gaatgcgcag ttagccgcct tgtttcagga tgattcggag ccgttaagcg agatgcgtac
    15061 ccgctgtgag acactgttac agacggcgca gaacaccatc agtcgcgact ttacgctgcg
    15121 ttttgccgag ctggaatcca ccctttgccg ggtgttaacc gatgttattc gccccattga
    15181 gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt cctgggtttc attttcctgt
    15241 ttttcacggc gtagttcccc acttcaacac tcgccagctg ttcagtgcag tcatttcgcg
    15301 ccaggatgca acggacgagc agagcacgcg tttaggcgtt gtgcgtgaga ctttttcgcg
    15361 ctggttgaat cagcccgact ggggacgggg aaatgagaaa tccccgacag aaacggttga
    15421 ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat ctttattgcc aacaaatggc
    15481 taaagttctg gcagagcagg tcgatgaatc tgttacggca ggcatgaata cttttttcgc
    15541 tgagttcgct tcatgtttga cggaattaca gacgcgttta cgcgaaagcc tggctctgcg
    15601 tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa ttgcagcaga ctgtgatgac
    15661 tcacggctgg atttacaccg acgcccagct gttacgcgat gatattcaaa cacttttcac
    15721 ggcagaacga tattgaccaa gacgttactg gacggccccg gtcgcgtgct ggagtcggtt
    15781 tatccccgct ttttagtgga tctggcgcag ggcgatgatg cccgccttcc acaagcccat
    15841 cagcagcagt ttcgtgaacg actgatgcag gaacttcttt cgcgtgtgca gcttcagaca
    15901 tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc gactgacaat ggtggaaaaa
    15961 ctggcgtcga tgctggatcc cggtcatctg gcgctgacgc agatcgcgca gcatctggcg
    16021 ctgctgcaaa aaatggatca ccgcaagcac tctgctttcc cggagctccc ccagcaaatt
    16081 gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga aggaaaaggc attaacgcaa
    16141 cgaggcctac tggtgcaggc gggtgaacag agcgagcaaa tttttacccg ctggcgtgct
    16201 ggggcgtata acgcctggtc gttgcctggg cgctgtttta tcgttctcga agagttgcgt
    16261 tggggggtat ttggcgatgc ctgtcgtctg ggaagcccgc aagcggtggc gttgttgctg
    16321 ggtgatttgc gcgtgaaagc gacacaacat ctggcagaga gtatcaatgc ggccccgacc
    16381 acgcgtcact attaccatca gtggtttgcc tcttcgaccg ttccgacggg cggggatcat
    16441 gctgattttt taagttggct gggaaagtgg accacggcag ataaacaacc cgtttgctgg
    16501 tcagtgaccc aacgctggca aactgtcgcg ctggggatgc cacgactctg ttcagcgcag
    16561 cgtctggcgg gggcaatggt cgaggaaatc ttctctgtaa atttggcgta aataatcagt
    16621 tacatcaatg agtcctaaac gaaatccatg tgtgaagttg atcacaaatt taaacactgg
    16681 tagggtaaaa aggtcattaa ctgcccaatt caggcgtcaa ctggtttgat tgtacattcc
    16741 ttaaccggag ggtgtaagca aacccgctac gcttgttaca gagattgcat cctgcaattc
    16801 ccgctcccct tttgcggccg tcgcgctgat ttttctggcg tttgcggaaa tgggccaact
    16861 ctgcgaggaa agctatgctg aaaaggaaaa aagtaaaacc gattaccctt cgtgatgtca
    16921 ccattattga tgacggtaaa ctgcgtaaag ccattaccgc agcatcactg ggtaatgcaa
    16981 tggaatggtt tgattttggt gtttatggtt ttgttgctta cgcattaggt aaagtttttt
    17041 tcccgggggc tgaccccagc gtgcagatgg ttgctgcact tgccacgttc tccgttccct
    17101 ttctgattcg accgcttggc gggctcttct ttggtatgtt gggcgataaa tatggtcgcc
    17161 agaagatcct cgctatcact attgtgatta tgtcgatcag tacgttctgt attggcttaa
    17221 taccgtccta cgacacgatt ggtatttggg caccgattct gctgttgatc tgtaagatgg
    17281 cacaaggttt ctcggtcggc ggtgaatata ccggggcgtc gatatttgtt gcggaatact
    17341 cacctgaccg taaacgtggc tttatgggca gctggctgga cttcggttct attgccgggt
    17401 ttgtgctggg tgctggcgta gtggtgttaa tttcgaccat tgtcggcgaa gcgaacttcc
    17461 tcgactgggg ctggcgtatt ccgttcttta ttgctctgcc gttagggatt atcgggcttt
    17521 acctgcgcca tgcgctggaa gaaactccgg cgttccagca gcatgtcgat aaactggaac
    17581 agggcgaccg cgaaggtttg caggatggcc cgaaagtctc gtttaaagag attgccacca
    17641 aacactggcg cagcctgttg acatgtattg gtctggtaat tgccaccaac gtgacttact
    17701 acatgttgct gacctatatg ccgagttatt tgtcgcataa cctgcattac tccgaagacc
    17761 acggggtgct gattattatc gccattatga tcggtatgct gtttgtccag ccggtgatgg
    17821 gcttgctgag tgaccgtttt ggccgtcgtc cgtttgtgct acttggtagt gttgccctgt
    17881 ttgtgttggc gatcccggcg tttattctga ttaacagtaa cgtcatcggc ctgatttttg
    17941 ccgggttact gatgctggcg gtgatcctta actgctttac gggcgttatg gcttctacct
    18001 tgccagcgat gttcccgacg catatccgtt acagcgcgct ggcggcggca tttaatattt
    18061 cggtgctggt tgccggtctg acgccaacac tggcggcctg gctggtcgaa agctcgcaga
    18121 atctgatgat gcctgcctat tacctgatgg tagtggcggt gattggttta atcaccggcg
    18181 taaccatgaa agagacggca aatcgtccgt tgaaaggtgc gacaccggcg gcgtcagata
    18241 tacaggaagc gaaggaaatt ctcgtcgagc attacgataa tatcgagcag aaaatcgata
    18301 atattgacca cgagattgcc gatttgcagg cgaaacgtac ccgcctggtg cagcaacatc
    18361 cgcgaattga tgaataagct gaaacggatg gcctgatgtg acgctgtctt atcaggccaa
    18421 ttgaactctt aaggttcact taatctctga cgcgcatact ctcctccagg ttaacggagg
    18481 agagtgcaat gaaaaaccgt gtttatgaaa gtttaactac cgtgttcagc gtgctggtgg
    18541 tcagcagctt tctttatatc tggtttgcca cgttctgatc tttcttcagc cgtacccagg
    18601 cccgcgtgcc ggaagtctct tgccggtttt gcaggaaaaa ctgcccgtga tgcaactgtg
    18661 taatgcggct gacaatactt aaccccagac caatcccgcc ataacggctg tccatacgta
    18721 caaacgcttt actcaactcc ccgcatttac tctcatcaat acctggtcct tcatcttcaa
    18781 ctgccatgac cgctccgtca tcttcttgca gcttaatcat aatgttgctg ccttgcgggc
    18841 tgtaacgatg ggcgttttct accaggtttc gcaataacat ccgcagcagg gttgcatcac
    18901 cctgaacggt gatgtcggcg gcgctctctg gcaatagcag ggtttgctgt cgctggtcga
    18961 gcatggtact gagttcgtca tacgagggga gaatgacatc ttccagcagt tttacatgtt
    19021 gataattacc ggaagaaaat gactgtccgg cacgcgccag ttgcagcagc tgggagacgc
    19081 tctccatcat ctgatcaagt cgtgccacta acggtgctac atcaatgtga tgcgttttcg
    19141 ccagcagttc cagatgcaaa cgcacccccg ccagtggcgt tcgcagttcg tgcgcgacgt
    19201 cagcggtaaa caacctttcg ttatccagcg tgctggtcag gcgactgacc agatcgttta
    19261 acgccgaaac caccgcttcg atttcgaggg tggcgctgtg aatggcaatg ggcgttaagt
    19321 tgtcggcagt gcgcgcttcc agctcttttt gcagctccgc cagcgggcgg gtgatgcggc
    19381 gcaccgcctg atagcagata aatagcgtca ggctgaccat aaagacgccg gggacaatca
    19441 ggctggcgac cgcctcgcgg atctcacgca tgatgtggcg atcgttgttg cgattgtcgc
    19501 gtagcgcctg ctcaaacagc tgaatctgct cggtactttc atgccatagc cagaagacgc
    19561 tgatcagctc aaacaccaac aaaatggccc cgatggtcaa tatcagccgt tggcgcagcg
    19621 atattggtcg gcgcagaaaa tgcatcagat tcaattagtt ttcctcattc gcgaccagca
    19681 tatagccaaa gccgcgcacg gtgcggatac gggctttgcc cactttgtcg cgcagattgt
    19741 ggatatgcac ttccagggtg ttggtcgagg gttcattgtc ccagttatag atgtcgttgt
    19801 agagaatttc ccgatgcacc ggactgcctg ctttgagcat taaccgtgac agcagagcat
    19861 attctttggg cgtcagaatc aactcttcac cgcccatcca tacctgacgg cgacccatat
    19921 tcagcgtcag attgccaaca atcagctcac tttcgccctg attattatgg cgtcgtagca
    19981 gggcgcggat acgggcgtgt aactcttcca gcgcaaaagg cttcaccaga tagtcatcgg
    20041 caccgacatc cagcccagcg attttgtcgg tcagcgtatc gcgagcggtg aggatcagta
    20101 ctggcagggt gtattttttc tgccggatac gggcgagaaa atgcagtcca tcttcgtcgg
    20161 gtaaccctaa atccagtacc accaggctgt aatgaccggc ctcaaggctt tgttccgcca
    20221 tccgcgcggt tgtcacgcca tcgcacgcgt agccttcagt ttgcgccgcc agaatcaatc
    20281 cctgcaataa cagcgtatcg tcttcaacaa tcagaatttt cattcactca ctctcctgca
    20341 agtttgcaga atatcatccg cagcctggta atacttcgtc tcaacgccag ttaatcccaa
    20401 tagcgtggag aataaattgt cttgtgaata gtgttgcgtt tgcgcctgtt tttgcaggca
    20461 gttctggtca acctgatacc gtttttgata atcctccgac agccacagca gcatcggcac
    20521 ctgtttttgg ctatccgggg cgatggcata aggcagaccg tgcagataga tgccattttc
    20581 acctaacgat tcaccgtggt cagaaagata aaccaggctg gtggtaaatt tatcctgatg
    20641 ttctttcagc agattaatcg ctttatcaac aatatagtcg acgtaaacca gcgtgttgtc
    20701 gtaagtgttc accagttgct ctttgctaca ggtctggatc tcattggtgt cgcaggttgg
    20761 ggtaaatttc ctgaactgcg gcggatagcg gttgtaatag gtcggaccgt ggctgccgat
    20821 ggtgtgtaag acaatcacgc catcaccctg caggttattg atgtactctt ccagcccgtg
    20881 gaacagcact tcgtcatagc attcgccgtt gatgcactga ccaggcagat tcagcgcggt
    20941 gacgttctgg tgaggcacgc ggtcgcaggc acctttacag ccgccatcgt tgtcattcca
    21001 cagcacgttg atgcccgctc gctgaatgat atccagcacg ccttcctggt gctgtgccag
    21061 ctcttctttg taatgctcac gcggcatatc cgagaacatg cacggtactg aaactgccgt
    21121 tgccgtgccg caagatgcgg tattagggaa atagaccacg ttatctttcg ccagccgcgg
    21181 gttagtttca cgcgggtagc cgttgaggga gaagttctcc gcccgcgagg tttcgccgac
    21241 aatcaggatg gtcaaatttt tacgtttttc gttctgcatt aacgggttgc ggtgggcgtc
    21301 ttcaccaatt cgcaccagcg gtagatttgc cagtcgctga tgggagtacc atgaccagct
    21361 ggcaacaatg ctgttagagg ggcttaagga tttcaccagc tctttgttat tgcggaacaa
    21421 cgaggcgtag tctttataaa acagtgcggc gaccagcaaa atcagtagta cagaaaccag
    21481 aatattggct ccacggaaaa gaacactgcg cagacgcgag gtggcaggtt tgatttttat
    21541 ccagcaggca atcagcgcag caagcacgcc gctgaatccc agcgttaata acatttgcgg
    21601 tgtcatcagc gcataacttt ctgccggagt ggtatcaata atattggcaa tcatcgagcg
    21661 gtcgatgacg atgccgtaag tcattatgaa atattgtgca gccgcgccaa ccagaataaa
    21721 caggcaggcc agtggtcgat taagccataa gaaagagctt agtgtcagga caatattaat
    21781 cacgctgaaa gcgacgaccg gcatcgacaa gaaaaccagt acgttatgca gcgaatccag
    21841 cggcagcgcc tgcaacacct gtttaaaaaa ggcaatattc aggcagatag agatataaaa
    21901 agcggccaac aatagccagg cgagtaaatt caaagagggt ctttttagta ggcgcttcaa
    21961 catcacggtg tttccatcga acaaagtgcg catatgctcg caaagcaaaa ttaagccaac
    22021 cttaagttct taaggttggc ttttatgttt gctggattaa ggggaattaa tctttgctta
    22081 ttggtgcatc taagggatac gggtttttat gtagccggtt gtaattcagc gcatacatgg
    22141 cggtgatgac catcagggtg acaaatgacc acataacctc tttcgctccg gaccccacca
    22201 cggcccagat gcagtagagg aaggcaatgg tagtaactgc cagatatgcc gggcgtgctt
    22261 taccaaagtg accgtgtccg agcagcagta acgccgcaca ggtgtaaaga tatggcacca
    22321 gtgtaaagat gaccgagacg gaagaaacca gaccgaactc tttggtcgcg tttggtgaaa
    22381 tgctgctgag ctggaatatg gtcatcaaaa taccgacgat aatcagcccc gccactggcg
    22441 tacccgcttt atttacacgg gcaaaaatcg gtgggaacag tccgtcatcg gcagcggctt
    22501 tcgccgtttg acccgccagc aacgtccagc cgcccagtga acctaagcaa cccgcagctg
    22561 cgcagaagga aacaatggcc ccggcggtgt cacccaacgc catccgtgcg gcatcaccga
    22621 atggcgaagc agaaacgcgc agtgcggcat tagggatcat ccccataatc gcggtggtag
    22681 aaagtacata gcaaacggcg gcaatcaata ccccaccaat ggtggcgata gggacattgc
    22741 gtttcgggtt tttcaccaca cctgcggcaa cggaggcact ttccacaccg atgaacgacc
    22801 acagcgtaac gttaagggta ctttgaattg caccgaaggt gcccaggccg ctgacgttcc
    22861 atgccgccat ataggtttca ccacggaacc agaaccagcc aaatacggca atcccgacga
    22921 tggggatcag cgccagcacg gtggcaactg cctgcacacg ggtgatcatt ttcggaccga
    22981 caatgttcag caggacgaag atccacagca ccacgacgca ggtgatggtt aataccaatg
    23041 gatctttcag aatcgggaag aagtaactta aatatcctac gccaatgacc accatggcga
    23101 tattgccgat ccagcaggcc agccagtaga ggacgttggt ttgataaccg agaaacgggc
    23161 caaagcagcg gcgggcgtaa gcgtaagaac caccaggact tggatcgagg aacgacattt
    23221 tggcgtatac catcgagagc cccagcgcac cgataatcgt caccaaccat ccataaatgg
    23281 caatcccgcc agtcgaggcc aggtttgcag gtaacagaaa aacacctgac cccataatat
    23341 tccccgacac catcagggtg acggggatta agcccacttt gtgagcatca gcatccgaag
    23401 acataattaa actcctgcga aggcgagctt cgtgacaata aattacgtca tatcatacgc
    23461 ctgcattcga tagttgaatt attatcgacc gggttatcgc tgatgccgtt attaatatag
    23521 ctcttcgtta aggttaaaaa tcaggcgaca gtgcgttctc tgtgctgact aacataatgc
    23581 agcggcgtca taccgtagaa gtctttaaag acagaaataa agtacgacgt actgttgtag
    23641 ccgcaggact gtgatacctg agagatattt ttaccgtcca tcattaattc atttacggca
    23701 taacgcatgc ggcaggtggt gattatttgg ctataactgg tgttttcgct tttcaacttt
    23761 ttctttaaca gacttgggct aagacataaa caactggcta ccatactaag attccagtct
    23821 ttgtgaatat cgctttcaat aatttgataa acgctgtcac ttacacaatt acgtaacata
    23881 tacatcatta atgaaaggaa ttttttactg tcgagaaaac gggatagcac cgtgaataat
    23941 aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg gcatcatgct gtgttgcgcg
    24001 gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga gtatgggcgt agcgctacgt
    24061 tgccatactg gtatttgcga gagatcttta ttcagaaacc ggaggtaatc tttgatgatg
    24121 tcgtgactaa tatgagcaac caaagtgttg ttaagagagg agacgtcgat aatattattt
    24181 tcacaattta ataacgccat atggttagct ttaaggctaa taggttcttt cccattcacc
    24241 cttatccaga catctttttc agtcaataaa acaatacaag gttggtcgct gcaaatcctc
    24301 atacatgtac tcctgagtgc gaataaaaga ccagttctcg ctaaagcaaa gcgataccgg
    24361 gaaatggtta tataattgta tgatgaatat aaacatgcat aaaaataacg agttttacta
    24421 atattacaaa tttttatagt aatttctcat gtacatattt ttttacagga taaataacag
    24481 gcaaaaaaaa gcgcggggtg gggccgcgca aaaagtatga catttttgtt tgcagcaatt
    24541 tttatgaaaa cgttgccata ctcaaaaggc ctaaggccag gcggcgtaat gttatttaaa
    24601 caattacgcc ttcagcggaa tagtggttac gctttcacgc acataacgtg gtaaataccg
    24661 tcaataattt cagtcccttc agtttcgtgt tcaaatccag ggaaatggtg gtcccaggat
    24721 tgcagcgagc gtaaataact tacttgcgga ctgtttttat cgccgaagtt ttcaccagac
    24781 agcagcatcg ggattcctgg cggatacggg ataactgagt ttgccgcgat gcgtcctggc
    24841 agattttcaa tggataccag ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgc
    24901 ggggtgactt ccgccaccgg caggccggaa taggcttcgt tcaaccgtgc gccagggttg
    24961 ttttctttca gccaggcaaa catggtgtca cccagatcgt gaatccccat gttcgcgtaa
    25021 gtgtcaggat attgttcaac aagttccggc atcacctgcg ccagcggtgt gttggcgtca
    25081 tagtggcgtt tgaaggagca aagggtgtta accagagttc cccatttccc acgggttacg
    25141 cccatagaga acaggaacat aatttggaag tcagtggtgc gggtaggtac aatgccgtgg
    25201 cgaccaagcc aggcagtgac cagcgccgcc ggaacaccgg tttcttccag ttcaccatct
    25261 tcacccattc ccggagcaag gatgctgact ttaatcgggt cgagcatact ccagttatcc
    25321 ggaatatctt tgaagccgtg ccagctttcg cccggatgca ttacccagca gtcctgaacg
    25381 gtggtcagca gtttggttgg tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg
    25441 gtgacgactt ctttgttcca cggtttgaag aaccagctac cgtcagcggt gaactcttta
    25501 tatagccgcg ccatcgcctg acggaaatca accgcttcgt caatcacttc ctgtgtcagt
    25561 gacaggccgc tgttgccgtc catcatcgac accgccacgt cgttggatgc gcagatggca
    25621 tacagcgggg aggtggtggc atgcatcatg taggcctggt tgaagcggga gaagttaatc
    25681 gccccacgac cttcacgtac atgaatataa gaagcctgtg acagcgcatt cagcagtttg
    25741 tgggtggagt gggtggcgaa aacggtagga ccgttgtgat cgccaggttc gccgcgcatg
    25801 gcatagtgat cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag
    25861 tgcagacgat cggaggtttt ttccagcaga tcctgcgctt ctttagcgtt ataacacacg
    25921 ccgtcatagg tgcagttggt caccacgcag taagacggtt tttgcccggc tttgtctttg
    25981 gtcagcgggc tttcactgat tttcttctgc aaggtttcag gttgcatttc ctgcggatag
    26041 attggcccga taatgccgta gcggttgcgg cttggcacca tatagaccgg tttcgcgcct
    26101 gtcagcatca aaccttgttc gatggattta tggcagttac ggtcaacgac cacgacatcg
    26161 ttatcggtca tgcaagcctg catgatggtg cggttagagc cggaagtacc gacgactacc
    26221 gaccaggagc gatcggcacc aaatacgcgt gcggcatatt tttcgctttc gccaaatgcg
    26281 ccagtatggt caagcaaaga accgagggaa gttcgttcga tgcccatgtc ggtgcggaac
    26341 agattttcac catagtagtc atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg
    26401 ccgccctggt ggcctggcgc tgcccaggaa tattcatgga tgtcactata tttcatcagc
    26461 gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg tcatcgcggc aacggcgcgt
    26521 ccggcgataa agtcggcggt atcttccaga atccaggcga attcatcgac aagctccagc
    26581 aggtcgcgat ccattgcggc gagggctttt tcccgatcgc ccaacaggaa gaccggcacg
    26641 ttttgttggc gctcatgaag cttaccgatc aattgtctga cgttttgatg ttcgtccgga
    26701 tgttccattt gatagctgaa catcaggcag tcaatggctt cgtttgaaga gagaatggca
    26761 aaaccatcat caaaggaggt ggatttaatc acggtaacat tttgctggct taaagcatct
    26821 gccagacgct caacggcgtt accgacccag gtgtcttgat ggagaaactc gctttcaaca
    26881 attaatactt tcatcattgc ttacccggtt atgaaggaat cttcgttgcg ggcaagtatc
    26941 ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac atttttgaaa tatttatttt
    27001 tataaacaat gcgttatgtg attattttaa attattttca ctgcttatta tcgacggcta
    27061 aactattttt ttggctgata ctgatatcgt ctttagccgg gaaacgtctg gcggcgctgt
    27121 tggctaagtt tgcggtattg ttgcggcgac atgccgacat atttgccgaa cgtgctgtaa
    27181 aaacgactac ttgaacgaaa gcctgccgtc agggcaatat cgagaatact tttatcggta
    27241 tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg taatgtactg tttcatcgtc
    27301 aattgcatga cccgctggaa tatccccatt gcatagttgg cgttaagttt gacgtgctca
    27361 gccacatcgt tgatggtcag cgcctgatca tagttttcgg caataaagcc cagcatctgg
    27421 ctaacataaa attgcgcatg gcgcgagacg ctgtttttgt gtgcgcgcga ggttttattg
    27481 accagaatcg gttcccagcc agagaggcta aatcgcttga gcatcaggcc aatttcatca
    27541 atggcgagct ggcgaatttg ctcgttcgga ctgtttaatt cctgctgcca gcggcgcact
    27601 tcaaacgggc taagttgctg tgtggccagt gatttgatca ccatgccgtg agtgacgtgg
    27661 ttaatcaggt ctttatccag cggccaggag agaaacagat gcatcggcag attaaaaatc
    27721 gccatgctct gacaggttcc ggtatctgtt agttggtgcg gtgtacaggc ccagaacagc
    27781 gtgatatgac cctgattgat attcactttt tcattgttga tcaggtattc cacatcgcca
    27841 tcgaaaggca cattcacttc gacctgacca tgccagtggc tggtgggcat gatatgcggt
    27901 gcgcgaaact caatctccat ccgctggtat tccgaataca gcgacagcgg gctgcgggtc
    27961 tgtttttcgt cgctgctgca cataaacgta tctgtattca tggatggctc tctttcctgg
    28021 aatatcagaa ttatggcagg agtgagggag gatgactgcg agtgggagca cggttttcac
    28081 cctcttccca gagaggcgag gggagcgtcc gagcatcatg aggccgaaaa ctctgctttt
    28141 caggtaactt attaccataa actcagattt actgctgctt cacgcaggat ctgagtttat
    28201 gggaatgctc aacctggaag ccggaggttt tctgcagatt cgcctgccat gatgaagtta
    28261 ttcaagcaag ccaggagatc tgcatgatga ctgcacccaa aattacattt atcggcgctg
    28321 gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt ccatcgcgag gcgctgaaaa
    28381 cggcgcatat tgccctgatg gacattgatc ccacccgcct ggaagagtcg catattgtgg
    28441 tgcgtaagct gatggattca gcaggggcca gcggcaaaat cacctgccac acccaacaga
    28501 aagaagcctt acaggatgcc gattttgtcg tggtggcatt tcagattggc ggttatgaac
    28561 cttgcacggt gactgatttc gaggtctgta agcggcatgg tctggaacaa accattgccg
    28621 atacgttggg gccgggcggt attatgcgcg cgctacgtac cattccgcat ctgtggcaaa
    28681 tttgcgagga catgacggaa gtctgccccg atgccaccat gctcaactat gttaacccaa
    28741 tggcgatgaa tacctgggcg atgtatgccc gctatccgca tatcaaacag gtcgggctgt
    28801 gccattcggt gcagggaacg gcggaagagc tggcgcgtga cctcaatatc gacccagcta
    28861 cgctgcgtta ccgttgcgca ggtatcaacc atatggcgtt ttacctggag ctggagcgca
    28921 aaaccgccga cggcagttat gtgaatctct acccggaact gctggcggct tatgaagcag
    28981 ggcaggcacc gaagccgaat attcatggca atactcgctg ccagaatatt gtgcgctacg
    29041 aaatgttcaa aaagctgggc tacttcgtca cggaatcgtc agaacatttt gctgagtaca
    29101 caccgtggtt tattaagcca ggtcgtgagg atttgattga gcgttataaa gtaccgctgg
    29161 atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg gcataaagag ctggaggagt
    29221 ataaaaacgc ctcccggatt gatattaaac cgtcacggga atatgccagc acaatcatga
    29281 acgctatctg gactggcgag ccgagtgtga tttacggcaa cgtccgtaac gatggtttga
    29341 ttgataacct gccacaagga tgttgcgtgg aagtagcctg tctggttgat gctaatggca
    29401 ttcagccgac caaagtcggt acgctacctt cgcatctggc cgccctgatg caaaccaaca
    29461 tcaacgtaca gacgctgctg accgaagcta ttcttacgga aaatcgcgac cgtgtttacc
    29521 acgccgcgat gatggacccg catactgctg ccgtgctggg cattgatgaa atatatgctc
    29581 ttgttgacga cctgattgcc gcccacggcg actggctgcc aggctggttg caccgttaaa
    29641 acgcgactaa acgctactgc gccgggggat ttattccggc gcacacctct gacgatacca
    29701 ataacagaag gcgggcgttg gtaacagcga cccgataccc tatgagcatt tcaatgacta
    29761 caaaactcag ttatggattt ggagcgttcg ggaaggattt tgcgatcggc attgtgtata
    29821 tgtacctcat gtattactac accgatgtcg ttgggctgtc tgtgggtttg gtcggtactt
    29881 tgtttctggt ggcgaggatc tgggatgcta ttaacgatcc gattatggga tggattgtaa
    29941 atgctacgcg atcgcgatgg ggtaagttca aaccctggat cctgatcggt acgttggcaa
    30001 actctgtaat cttatttctc ctctttagtg cgcatctgtt tgaaggtact actcagattg
    30061 tctttgtttg cgtgacctac atcctctggg gcatgactta caccattatg gatattccct
    30121 tctggtcgct ggttccaacc atcacgctcg ataaacgtga gcgcgaacaa ctggttcctt
    30181 atccgcgttt ttttgccagt ctggcaggct ttgttacggc aggtgtgacg ctaccatttg
    30241 ttaattatgt cggcggtggc gatcggggat ttggctttca gatgttcact ctggtactaa
    30301 tcgccttttt tattgtttca accatcatca ctctgcgcaa tgtgcatgaa gtcttttcgt
    30361 cagacaatca accgtctgct gaaggaagcc atctgacact taaagccatc gttgcgctaa
    30421 tttataaaaa cgatcagctt tcatgcctct tgggtatggc tcttgcttat aatgtagcca
    30481 gcaacattat taccggattt gctatctatt atttctcata tgttatcggt gatgcggatt
    30541 tgttccccta ttatctgtcg tatgcgggag ctgctaacct ggtgacgtta gtattcttcc
    30601 cacgcttagt taaatcatta tcccgacgca ttttatgggc cggagcatct attcttccgg
    30661 tgttaagctg tggtgttctc ctgttaatgg cattaatgag ctatcacaac gtcgtcctca
    30721 ttgtgattgc gggtattttg ctgaatgtgg gaacggcgct tttctgggta ttacaggtca
    30781 tcatggtggc agataccgtt gattacggtg aatataaact gcacgtacgc tgtgaaagta
    30841 tcgcttactc cgtgcagact atggtggtga agggcggttc agcctttgcg gcttttttca
    30901 ttgcggttgt gttagggatg attggctatg taccgaatgt tgaacagtct acgcaagccc
    30961 tattaggtat gcagtttatt atgattgctc taccaactct gtttttcatg gtaacgctga
    31021 ttctctactt ccgtttctat cgcctcaatg gcgacacgct gcgcaggatc cagattcatc
    31081 tgctggataa atatcgcaaa gtaccgcccg agcctgttca tgctgatatt ccggtcggtg
    31141 cagtgagtga tgtgaaagcc tgacgtggac aatagctaac gaaaatggcc tgatgcgata
    31201 tgtttatcag gccacgtctt aatgtaatgc tttggatttg caaattttgt agaccggata
    31261 aggaattcac gccgcatccg gcatcaacaa agcgcacgtt gttatccggt tatcaagcca
    31321 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
    31381 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
    31441 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
    31501 cttatcgcca gcgccacgca gcagaccagg aacgccacca ttttacgtcc gcgcacgtac
    31561 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
    31621 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
    31681 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
    31741 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
    31801 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
    31861 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggcttgagt
    31921 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcgtg
    31981 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
    32041 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatc tgctggaaga
    32101 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
    32161 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
    32221 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
    32281 acgcgtcggt aacctgctga ctgcggttac ctttcgccag catgatcatg ctgccgccgt
    32341 gggattgcag cagatccacg taggagtcca tacggcctgc ggtggttggg ccaagtgaac
    32401 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
    32461 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
    32521 ctcggcccac gataatggtg ccggtgagcg acaaacgagt ggataccggg tattgcgaaa
    32581 gctgggcgag gatctctttc atcgggcggt taaggtcaac tttcaccgct tcgccttcac
    32641 cggcctggcg cagttcttgt ggaatgtact ggcctgggtt gtgttccagt ttttcgatcc
    32701 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
    32761 tgccgaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
    32821 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
    32881 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
    32941 cgtaatagtg agcgcttgct aacttgacgg ttttcaggtt ggtttccgca gacgtaccgc
    33001 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
    33061 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
    33121 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
    33181 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
    33241 tgtccagcgc cgcattctgt gaatagcgca ggttatcttc gatataggtg ttatagacgc
    33301 ctttcgacag cgcttcttca tcaccgccgc cggtccacac gcgctggcct tttttaccga
    33361 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
    33421 agtttcttaa gaattgcagc gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
    33481 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
    33541 cttgctgcgc cagtagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
    33601 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
    33661 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
    33721 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
    33781 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc taacaccaat
    33841 caaccccggc agaataaaac tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
    33901 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
    33961 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
    34021 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
    34081 aatggcatac gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
    34141 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
    34201 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
    34261 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
    34321 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
    34381 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttatcgag
    34441 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
    34501 gaactcttcg tctttatcca gatctttacc gcggaaccag ctgaagatac cgatcgccag
    34561 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
    34621 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
    34681 actggcgata atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
    34741 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
    34801 cgtaccgcaa agaatggtca gtgtacaggt cacaaacggc gcgacaattg agacatattt
    34861 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
    34921 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
    34981 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
    35041 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
    35101 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
    35161 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
    35221 tggtcgttac cggctttagc aaatacctca cagtaaatat tggctgatta aagcagcggc
    35281 aaatttatgc actgtgataa gcggcttttt cagagagaaa aactctctgg cgatgggttt
    35341 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
    35401 gtctattttt tgttctcagg gcagatatca ttaggtttaa agattaatct gatctaccca
    35461 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttatcg
    35521 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatggaggg
    35581 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
    35641 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
    35701 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaaccg gacgccccgt
    35761 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
    35821 acggcaggaa acacgcgaga tgttaacctc gttagccagc tcgtcggttg aaaactcata
    35881 gtcctgatgc gcgtcaatcc actggcacag cgtgcgtaac gtctgcggcg ttaagccttt
    35941 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
    36001 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agccggtgag
    36061 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
    36121 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
    36181 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
    36241 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
    36301 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
    36361 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
    36421 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
    36481 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
    36541 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
    36601 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
    36661 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
    36721 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
    36781 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcggt taatcttgct gattaaaaaa
    36841 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
    36901 ttggctgttt tgagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
    36961 agaatcacat gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcagcatag
    37021 ttgaccagac catcgagtcg ctgcatcagt ttacgtactt cagttttgtc cctgaaggtt
    37081 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
    37141 cgatctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
    37201 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
    37261 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
    37321 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgctg ctcaaacagc
    37381 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
    37441 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
    37501 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
    37561 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
    37621 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
    37681 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
    37741 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gattccgctc
    37801 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agtcggcgag ggtacgcgcc
    37861 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
    37921 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
    37981 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
    38041 ttccttgtgt gacaaatttc ttaagcatta tctctgatga ggcgggtaat tcaaagggag
    38101 taagaatgat tggctatata ggggaagaga ctctggcaac ggaaactgcc agtgctgtat
    38161 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
    38221 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
    38281 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
    38341 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgtcact gtggttaaag
    38401 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
    38461 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
    38521 tgaaattgtc tttgtgccga ccggagagaa tttagcgatt atcgaacata ccgatgtcga
    38581 tgaaagcctg aaagggcaag ggattggtaa acagctggtt gcgaaagtcg tggaaaaaat
    38641 gcgtcgggaa aaacgaaaaa ttatcccatt atgtccattt gcgaaacacg aatttgataa
    38701 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
    38761 ttgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
    38821 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
    38881 ctcctatatg agaatcatca atcggggtta ataagttttg cgtccccaga gcattcaata
    38941 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
    39001 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
    39061 atttctcctc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaactcct
    39121 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
    39181 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
    39241 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
    39301 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
    39361 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
    39421 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
    39481 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
    39541 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
    39601 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
    39661 aatacccaga ccagcggttg gcggcagacc atattccagc gcagtcacgt aatcttcgtc
    39721 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
    39781 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
    39841 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
    39901 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
    39961 atcaaagatc tccgtgacaa tacgtcccag cccccagctt ttctctaccg taattccgat
    40021 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
    40081 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
    40141 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
    40201 cagggactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
    40261 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
    40321 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
    40381 acgcaggtac atatctaaat ccagagcgtt atgatgggta ataaacgggc gagcagatgc
    40441 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
    40501 gaactggcgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
    40561 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
    40621 gaatttatct ggtaaaggac gtagtgcttt tgtcagcagg cgtaactcag tacagtgaat
    40681 ggaaagctca ccggtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
    40741 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
    40801 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
    40861 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
    40921 atccttcgca tcaaactctt cgtgcagctg gtcagaggta tggtcgcggc gaaaatcatt
    40981 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
    41041 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
    41101 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
    41161 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
    41221 tcactaataa ataaccatca aaatggttta aatgacttat taatctagat ttgctagtgt
    41281 tataaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    41341 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    41401 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    41461 gcaattgcca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    41521 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    41581 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttcggcaaa gcccattagc
    41641 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    41701 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    41761 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    41821 ccagcgagca tcaccgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    41881 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    41941 tgtgccagta cccagaacaa tgtcccgaca aacatcaaca atacaatttg ccaaagagca
    42001 cggcggtgtt cggggaattt gaccatcatg cgggcaatga tttgtgcggc aatgaggcaa
    42061 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    42121 gctaaacaga gcatcaccac taaccagctc cataccggta aggcaaattt gacgctagtg
    42181 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgcccgct taagaaaatc
    42241 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    42301 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    42361 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtaaagctc gccaagcaaa
    42421 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    42481 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    42541 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    42601 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    42661 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    42721 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    42781 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    42841 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    42901 gtatgcaccg tgaatatcgg tttcaaagcc cggatagtga gcgccgattt cacacagcat
    42961 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    43021 aggaactccc ggcggatatg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    43081 gaggtaaact tcttcggtca taccgtgcag ctctttctgg aacgcagcat acggagtcat
    43141 taccatcgtc ggcagcactt caaatgcgcg atacatcaga tccggcagat tgtggtgaac
    43201 aatcagtttg tggatattct gagccagttc ctgaatacgc atgttttcat agaattcagg
    43261 atcttcacga tacagagacg gcagcatgtt tttcacacgc aggttcaggt cgaacgcacg
    43321 tttgaagtca gtcagagcac gcagcaggct cagtgctttg gtcttatcga taccgatgct
    43381 gaacaggaac agcaggttat acggaccggt tttctcaaca acgatgccat gttcgtcgag
    43441 gtatttcgcc acgatgctgg ccggaatacc aaagtcgctc atggtgccgt ctttttccat
    43501 ccccggagtc agcagggtga ctttgatcgg gtcaagatac atgtgctcgt tatcgatgtt
    43561 tttgaagccg tgccaggtgc tgtcagaacg cagcggccag cattcagtgg tatcgatatg
    43621 atccggctgc catacatcaa agaaccagcc atcagattcc gttctcagac gtttgatctc
    43681 tttacggaat ttgatcgcac gttcaatgga accgttgatc agacgcttac cagcattgcc
    43741 tttcatcatc gccgctgcgg tttcagtgga cgccacgata ccgtagtgcg gagaagtggt
    43801 ggtgtgcatc atgtaggctt cgttaaaggt ttcttcgttt acgtcacctt taacgtggat
    43861 catggaagcc tgagagaacg ccgccagcag tttgtgagtg gactgggttt cgtaaatcac
    43921 tttcccttct acacggccac cgctcatacc gcatttacct tcgtaaatcg gtgagaagtt
    43981 ggtgtaaggc acccacgcgg agtcaaagtg gatggatttc acatccagtg ttttcttgat
    44041 aaagtcggtg ttgtacagca gaccatcata ggtagagttg gtaattacag catgtaccgg
    44101 ccaggttgcg tttggtgttt ctttcacgcg cttagcaatg gtagcgtgct ggaattcact
    44161 ctgtgggata ccaccaagaa taccgtaagc gttacgggtc gggcggaaat agattggcgt
    44221 aacatcgctc atcatcatca ggtgggtcag cgatttgtgg cagttacggt caatcagaat
    44281 ggtgctgcct gctggagcag agtacatacc aacaattttg ttcgcagtgg aagtaccgtt
    44341 ggtcaccatg tagctgcggt ctgcgttaaa gacgcgagcg atatactgtt ctgcttcttt
    44401 gtgtggacca ctgtgatcca gcagagaacc cagttcagat actgaaatgg aaatatcaga
    44461 tttcatggtg ttcggaccaa agaaatcata gaacaggcta cctaccgggc ttttctggaa
    44521 tgcagtaccg cccatgtgac caggagtaca gaaagtatat ttaccttcac gaacatattt
    44581 aaacagtgct ttagtcagcg gaggcagaat agtgttgata tattcgtcag tggtctgctt
    44641 gatcttatta gcaatatctt cagcagcacc cagcgcatat tcaaagaagc taatctgtaa
    44701 acgcaggtca ttcaggctta catcgagagt ggaatacgta ttagcgaacg cgtacaacgg
    44761 caggttctcg ttcattttgc taatttcttc gcacagctcg agattatatt tatcccagtc
    44821 aaaaataacg ccgcacagac gcgcattgtt ttcgatcagt tttaataagt cgtcacggtc
    44881 gttcgggtaa acaatctgga agttcagacg ttcaagcgcg cgatgaagtt cacggatggg
    44941 ttcttcttta aaataaaccc ccatgtgatt caatattgca ataacgttca tagtcatatc
    45001 tccaggtaaa aaaggcccct cccaacacat gggacaaaat gaaaggagga gcctcggaaa
    45061 atacttttaa ttaatgtgcg ttagacgcgg tgtggttatc cattgagtgg ctctggcgct
    45121 cgtgcatttt gcgagcgtag aacatcagga taatcaggct gacgatgaag gtacctgcca
    45181 gctcgaagga gcttgcgccc atcagcgcga tgaagcagaa cacacaaccc agtacagagc
    45241 agatcaggct gacaaagttg cggatgttaa cgccttcaaa acgaatcagg tcaacgcaag
    45301 agtagaagta cggcagcata gtcagcagta ctgcgatacc ggtcagttca ccgaacaggt
    45361 cagatgcttt accaccggca gagttcatca gagtgatgag gatcatcagg gcagtcattt
    45421 tcactgcagc cagcagcaga ccttttttcg gaataccgtt gctgtcgact tcaccataaa
    45481 ctttcgggaa gttaccgtcg ttagcggcac gtacacctgc ctggcctacc aacatcatcc
    45541 aggagcccag agaagtcagg cacgcaaagg cggtgaatgc agaaaccagc ggcgcagccc
    45601 agttaccgag gatggttgaa gcactgattg caaacggagc accggaagct gccattacag
    45661 aagatggata cataccggaa agcacctgag tcgcagcgat gtaaacaata cctgctaaac
    45721 cagtacccag catggttgcc agcggaacgg tacgtttcgg gtttttaacc ataccagtac
    45781 ttactgctgc ggattcaaca cccacgaagg cccacaggca gagcagaata cttttaatga
    45841 tcgcatgacc atcagtggta tccgcagtat tccagttagc tgcataagtt gccgcatcaa
    45901 accaatgcca gccaacaata gcggtcatta ctaccggaat aagaaccagc accagaccaa
    45961 tagtggttaa acggcttacc caagtaccgc cgagcatatt tacaaaggta aatacccaga
    46021 cgatagcaat acaggcgata cccgccggaa caggatcatt taatactggg aagaaggtgg
    46081 aaagataaga taccgcggta ataccaatcg ccaggttacc aatccagtta gcatggtaat
    46141 aaagaacacc tgtctgaaaa ccaaatgcag gggaaatttc tccggcataa gcaattgggc
    46201 caccttgttg cgggtttttt gttgccagtc gggcatatac atacgccagc gacattgcac
    46261 caataataga gataatccaa ccccagatag caataccacc gatacttgct aggttcgcag
    46321 gtaataatgc aataccgctc cccatcatat taccggcaac aacaccggta caggcaaata
    46381 gcccgatctt cttggcagaa ctcatgctct tctcctaatt tcatttttga atttggagtc
    46441 cgggtcatga tgtataacta tttcctgacc agaccaaact ggcgctaaga atactcacga
    46501 aaaaaggatt aatcctaaag attaggtgaa ataacacaaa agtttctgta agtgagaact
    46561 tgaggttttt tattaacaca tcaggatcgc aagttgatat catgaaaaga taaacattca
    46621 atgtttacaa tggattgcgg gatattctct ggttaaattt atgtgataaa aattatgcgg
    46681 caaataaatt gccgcaacat attataccaa tagaaacata caaaaactca acaacaaata
    46741 ttcccgagca taaatcaacc cgggagttac ttattctgaa gcgagaaatt tgtcgagata
    46801 aggtacaaca taaggaacag aagtctggaa tataccattt tcaatccagt aaagggtgtt
    46861 tgcccctggg cgtaaattaa aggcggtgag atatgcatca gccgcttccc ggttcatccc
    46921 cttcatttca taaaccttgc caagcaacac ataatttagc caggacattt caagatcaat
    46981 gccagtattt atcgcctggt aagactcatc tgttttacct tttaccagag cactgaccgc
    47041 ttttatttga tatataatgg acaggttatt caattccggc agtgtaacaa tgttgtctat
    47101 ttctgtgttc agtgctgcta attgtttttc atctaaagga tgttgagaat ggcgcacgat
    47161 atcaactaat gctttttctg ctctcgcgta ggtaaattct ggggatgatt gaacaatctc
    47221 acctaataat tcactggcac gattcaatga tttatcatcg ccatgcagta aataatcatg
    47281 tgcctgataa aaattagtta ataacgcacc acgatgaggc aatatttgct ggagcgtctc
    47341 ctgcattcgt tgtggccacg gttggtttaa cgcttttgat aaactctcca gtaaatcatt
    47401 ttgaatcgcc agctgattac cgttagtgat gacataacgt ttatccagca tggttgaacc
    47461 atctgcattg tctaccaatt ttatcgacat aaagcattgt tgagcacggt attggcgctg
    47521 attaacaaac gcaatagata atgttttacc ggaactgctc ggttcatcaa tgttgtagtt
    47581 gattttgtca tgcaccataa aggtggagaa ggtgttaagt gatgtcgcca ccaaatcacc
    47641 cacgcctatc gcgtaagaga gctgatacgg ggaactccag ctgttacaac ttttatttac
    47701 catattaatg tcaatatcgc gtggattgag caaaatacgc gatttgctca taggaagacg
    47761 tgtatcaaga gttgaaaacg ctaccagtgc tacacagata cctaacgaca acaggaaaaa
    47821 aaaccatacc caaaaggtag tgaatcgttt gcttttaact ggggattgtt caggtggcgt
    47881 tgcggtgttt tgaatgttaa gactgtggga gggagaatct gtggcaggaa ccgcctctgg
    47941 tataggggga ggcgaagata gcattatttc ctctccctct tcttcgctgt accagataac
    48001 cggcaccatt aatttatagc cgcgctttgg tacagtagcg atatagacag gactatcttc
    48061 atcattatct tttaatgact tacgtagttc tgagatactc tgcgtcacaa catgattggt
    48121 gacaatactt ctcttccaga cattatcgat aagttcatcc ctgctaagta cttcgccact
    48181 gtgttgagca aagaaaacca gaagatcgat taatctcggc tcaagggtaa gttgacgccc
    48241 attgcggcta atttggttta tggacggagt aacaagccat tcgccaacgc gaactacagg
    48301 ttgttgcata atagaaactc attcgaaaag ggaatgatgc aatgataatt gccacaacct
    48361 atttttacca tctatagatg ggtttattta catattattg gtgaatgcaa gacgttattt
    48421 ttaccagcca taaacttctt gattacatag tattacgaaa ggattttact gagaaccaga
    48481 agtaatattc cttaccatca aaattcatca tctttgccaa agaaaaatgt tcagaaaata
    48541 atccatggaa aattgtccgg agcgcttact attttaatgg attgttagtc tttgcatgag
    48601 caagcggact gatacatttc tctttgttct cattcagaaa atctcatcag tcggcgttca
    48661 gaccggtgtt gtgctttatg gatgcgttac gctcctgatg acgtcatttg acgttcaaca
    48721 acatcacggg gccgcacgac atttcacgtc agttagtgct atcgctcagg aacaaatttt
    48781 cccgaattgg gatatgcccg caaattgctg gtgatgtggg gagaatctgg ttgagttcgg
    48841 gagaattgat ttggaggcag aacgcttaaa ttgtggcgtc cagaaacgaa aaacggacct
    48901 ccgtggaggt ccgttgatat gaatttggtg ccggactcgg aatcgaagaa tgcttctatc
    48961 atgttgatat tattttattt tgtttggttt ggtgaaatat gtaccctctt ttgtacccgc
    49021 agttcctttt gatatcgaaa gggaatcaaa ctcggggtca ggctgagagc acagaaggaa
    49081 agtgtctggc tattatatcg ttaattctgt caatcagctc cagccttttg aaagtgatgt
    49141 gtgcagatcc acccttctta aagtatttaa tgatgaacat ctcatcttca tagcgtcctt
    49201 taccctgaac ggactgtatg tggtcatcca gattaatggt gatattccct cggttgtcgg
    49261 ggatcggttt accgctgagc agatgcaaca ttctttccag gtcagccagg cgatctgcct
    49321 gcattccact gataagatga aatccccact ggtcccatct caccagattg ttgacgataa
    49381 ttttactgcc aaatttacag ggtgagttgg ttttgtaatc ccagctcagc cctttgaaga
    49441 cgttgatcac tccccgttca aacacttcat ctttgttctg gtgcagttgt tcaaatgtgc
    49501 tcagaatgtt cgcttcgctg atttccggaa aattatcata ttccagtgac ctggacctga
    49561 ctgaggtgga gcagataacg tccgtattct cgttgaccac ttccggacgg gagactgtga
    49621 tttcagacat aatcgatatt cctttttgca gataaatgaa aacgccagcc gtgttcaggc
    49681 tggcgtcagt ggaatgaagt ctggtgagtg agatgatgag tgagactcac cggttctggt
    49741 gctgaagctg cagttggcgc agggtacgga tatcctctct ggcctgttcg atacagggca
    49801 acagtgctgc tgaatccgtt ttatcctcat tgttgtcaaa gataattccc gattcgcagt
    49861 cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt
    49921 tcatgttgtt tctggttacc agtcgaaagt gggtaaaccc ggcaggcatt cagcgttggc
    49981 atccagatga atgagactga cgccataacg tcgggtaagt gtgacaacca gacgacggaa
    50041 cgtttttgac aggcccaggc gcttaagccg cagaaccggg tacgaccacg catcagtacg
    50101 taacagataa ccggtacctg taaaatgaat ccattctgat tcaccaacgt ccctggtctg
    50161 gtgtgacagc gagtacaacc aggcgttgtc cgtttccgtg atatgtgcgg tactgcagcg
    50221 tatgccggca agggttgcaa atggcggagg tgtgcaggtt gactgttggt cggattcctc
    50281 caccacacgg agtgaataac cgttttcagc gacgttatta atcagttcag tgagattaac
    50341 accatcgacg tcaacgacaa tgcgccccat gttcagtgcc tgcacgttaa cgctgtcagc
    50401 ttccagcgtc agggccagtt tcatcgtttc gcctcctgat acttacccag ggtaatgtta
    50461 tttaccgttc ggtaattgtc gcgggtcatc aggccagtcg cccggcgagc ccggaggata
    50521 tcgatgctgt ttattaactg agagcgggta caggcactga attccggctg gtcggtacgc
    50581 accagtgcgt atttttcaac aagaaagttc actgcatcgc acagtgaaat gccagcttca
    50641 atatgctgct caatcacacg ttcatcggcg aacggtgtgt cattcagtgt aaggccgtaa
    50701 tgctggccca gtagtcggga gagcagagtc tgccagatgg tgacgggaga cgggcagctc
    50761 gatgcctccc gtacgtgtgt atcgggtaat gttttcatac tgaaggtttt cctgatatgc
    50821 agagatgaaa aatggaaaag tggcgtggtg aaagcaccag gccggagcag aaggttattc
    50881 cggtggttta ctttttcgtt tcgggcgtcg gataaacagc cagataaacg taaccaccgc
    50941 taccgagagt gtcagctttg caggtcagac cttttgcata cagcgtgacg gtatgctgat
    51001 ggcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa
    51061 aggcctggtc caggtggtac gcatctgcat cgctgaaccg gcctctgata ccggcacggt
    51121 cggcaaggta atgcaaccgg ttaccctcct gcaccagacg ggcgccgaaa cagggcgtca
    51181 ctgtgcaggg tagtccccac cagggatggt cgtcattgtc atcgggatgc gttgtcccgg
    51241 aaagtgtgtc tgacacgata aaatcctcac agaaaatcgg tgaatgatag tttaacgatg
    51301 acgggtcagc cggcgcgcag accggcttcg gtaaaggtgt attcgttgat gagcaggacc
    51361 tcatccacag cggcgtctga gctcagccag tcgtactcgt tttccgggtg acggaacatc
    51421 agttcagggt gacgctcatc agccggacat acggaccaaa actgtcctta cggcgttctg
    51481 caaacacggc cagcacaccg ggaatatcct gcacttcacg accggtatac gcttcagcac
    51541 tgccgtgcca gcggtatttg ccggtacaga acggaaaaag acgggatgcc ggatgctgac
    51601 ggtgaatacg catggcttcg ccacgggtga tgattttcat agtgggatac ctctgaagac
    51661 agaagataaa agtgaaaaca ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc
    51721 ggcaaaggag aaaacctggc tgccaccgac tatcagatgg tccggcaccc ggatatccac
    51781 caggcccagt gcctgtacca gacgttccgt gataaggcgg tctgccttgc tgggtgtgac
    51841 ttcaccggac gggtgattgt gtgccagcac cacagcagcg gcattgtgat acagggcgcg
    51901 tttaatcact tcacggggat ggacttccgt gcggttgatg gtgccggtga agagggtttc
    51961 accggcaatc agctgattct ggttgttcag atacagcacc cggaactctt cacgctccag
    52021 tcccgccatg ttcagaatca gccattcccg tgccgcacgg gtggaggtga aggccacacc
    52081 gggttcatga agatggcggt ccagggtttt cagagcccgc agaatgagac tgcgctcgcc
    52141 gggcgtcatt tctccgggca gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc
    52201 gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc gcagccggta
    52261 ataatggacc gtcatggcat aatactccgt acgacaggcg tgatgactgt acgtcatcag
    52321 acaggcagca atgccggcgg cttccgggct catttcagcg cggttaccgt tcatggcatt
    52381 gaacagtacc catgtttcgc catcatcgtc atccggttcg ggcgctataa atgcgccacc
    52441 gttgttcagt gtgtacagat tccagatacc accgcagtag tcttcgcaaa gacggtccat
    52501 ccagccgaag atacggggct ccagggtcac ccactgtgga atgaggccaa aatgctgcgg
    52561 ccagaagctg atgcgctgtt catcagggac tatggtggca accagctgag gctggtcatt
    52621 ccctgatgca gcggttacgg aaacagaaga tgtggtggaa ttatgtgaaa tggttttcat
    52681 gaaatgactc cttaaaggac aaataaatgc gtggttttca gggtaatggc tgaatcagcg
    52741 caggctctcc agcagggttt ccgccatcac ccacaatgcg cggttgagct taatgtcagt
    52801 gtcaatgctg tgaatggcac gggtatggat acgttttcct ttagcgctgc gacctgaaat
    52861 tccgcctttc agcatattct cctgaatggt ctgataagca ctccacaggt ccttaccgta
    52921 atcctcccgg cgtcgtggcg tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc
    52981 ataacggtaa gtcagtgccg cctgtgccag cgcctggcgt gccggtggcg gcagaatcag
    53041 cgactgcatg gcatcacgct tttcctcaat ccggtcaaaa acccccacca cctcgtaagc
    53101 cccttcaatg actctgtcca ccacatttcc ccggtgcgga acacgcactt cccccagaga
    53161 ctgaccacag acgcatccgt tctggcagac gaacctgaag taacccggca gcatctggta
    53221 gctggaggta ccgtcatgag agttgagcag aataatttca gggacatgtt ctccgtttat
    53281 ctctccggcc cgccgcagac gcagcatgtg ttttgtgtat ccccggcggc ccgggtcgcg
    53341 cacacgggtc tggcaggcga agaatggctg aaagccttcc cgctgcaggc tttccagtac
    53401 ggtgatggtg gggatgtacg tatagttctg gcttcgggac gtgtgtttat cttccccgaa
    53461 aatccccggc acatagtgca tcagttcttc gtgtgtcagc ggacggtcac ggcgtatctg
    53521 gttcgcataa ccaaaacgac tggctagtcg cataatttgc tccttatcgg tggttaagat
    53581 ttactggtgt aataaatgaa aaagccacgt ctcccggaga agacgcggcc tgatgggtga
    53641 aatgaatgac gtttattgtc tgagaagacc gtcactggcg agccgcgtat taagcacctt
    53701 tccggcatcg ggttcagaat tccggcagcg ccagcgtcgc tgaccttccg cgttgcggat
    53761 aacccgacgg aaatgcattc ccgggtcatc ctggataaac acattgcggt aagctgttat
    53821 gacagcaacc gcctgcccgt atgagaaaga tccttcagcc aggacatact ctgtgtaacc
    53881 cggcatatct gttttctcct gataaataaa aagcctctgc cacgcgttgt gacagaggct
    53941 gaagcatgaa actgacttca gggatcagtt aacgtttttt ccggaaacgg taatcagcag
    54001 tggatggtag tcctggggat tgaaaaccga taacggcaga ctgacacgat ggccgttact
    54061 ttcttcagtt gctttaatga tttcggttgt ggcgacattt tccacgcact ccgtttccag
    54121 aaatgcgtct gtggttcgcg tggcattact gtcaccaaaa gcttccgttt ccatttttct
    54181 ggtcaccagc gtctgaccat atttgtcttt gagttgcaga gtgatggtga gggggccaaa
    54241 tccttcatcg tttccgccat tatccagccg gaactggtaa gcacaaatat ttcccgggag
    54301 ccatatcgta tctgtattgc gtatactgat gtaacgttga tcctgtgccc ggagtggggc
    54361 agaccacgtt aaccccagaa tgaaggcggt aatcatgcag gttttgaaca ggtgaatcat
    54421 ggtatttacc tctctgagtc atgacgatga cactgacaaa tcaggtgata aa
//
LOCUS       DAZGCS010000019        51867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGCS010000019 DAZGCS010000000
VERSION     DAZGCS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.019"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(74..1078)
                     /gene="argF"
                     /locus_tag="ACHK01_001228"
     CDS             complement(74..1078)
                     /gene="argF"
                     /locus_tag="ACHK01_001228"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO8096208.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1240..1656
                     /gene="rraB"
                     /locus_tag="ACHK01_001229"
     CDS             1240..1656
                     /gene="rraB"
                     /locus_tag="ACHK01_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO8096209.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1702..2205)
                     /gene="yjgM"
                     /locus_tag="ACHK01_001230"
     CDS             complement(1702..2205)
                     /gene="yjgM"
                     /locus_tag="ACHK01_001230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8096210.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSQSDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2398..3594
                     /gene="yjgN"
                     /locus_tag="ACHK01_001231"
     CDS             2398..3594
                     /gene="yjgN"
                     /locus_tag="ACHK01_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO8096211.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3650..6505)
                     /gene="valS"
                     /locus_tag="ACHK01_001232"
     CDS             complement(3650..6505)
                     /gene="valS"
                     /locus_tag="ACHK01_001232"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO8096212.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6505..6948)
                     /gene="holC"
                     /locus_tag="ACHK01_001233"
     CDS             complement(6505..6948)
                     /gene="holC"
                     /locus_tag="ACHK01_001233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO8096213.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7082..8593)
                     /gene="pepA"
                     /locus_tag="ACHK01_001234"
     CDS             complement(7082..8593)
                     /gene="pepA"
                     /locus_tag="ACHK01_001234"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO8096214.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8747..8797
                     /gene="ytgA"
                     /locus_tag="ACHK01_001235"
     CDS             8747..8797
                     /gene="ytgA"
                     /locus_tag="ACHK01_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO8096215.1"
                     /translation="MSIIILATISTTQENK"
     gene            8860..9960
                     /gene="lptF"
                     /locus_tag="ACHK01_001236"
     CDS             8860..9960
                     /gene="lptF"
                     /locus_tag="ACHK01_001236"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8096216.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9960..11042
                     /gene="lptG"
                     /locus_tag="ACHK01_001237"
     CDS             9960..11042
                     /gene="lptG"
                     /locus_tag="ACHK01_001237"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8096217.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11161..12663)
                     /gene="yjgR"
                     /locus_tag="ACHK01_001238"
     CDS             complement(11161..12663)
                     /gene="yjgR"
                     /locus_tag="ACHK01_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO8096218.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQKNPPTKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12793..13812)
                     /gene="ahr"
                     /locus_tag="ACHK01_001239"
     CDS             complement(12793..13812)
                     /gene="ahr"
                     /locus_tag="ACHK01_001239"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO8096219.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            14008..14092
                     /locus_tag="ACHK01_001240"
     tRNA            14008..14092
                     /locus_tag="ACHK01_001240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14042..14044,aa:Leu,seq:caa)
     gene            14279..15523
                     /locus_tag="ACHK01_001241"
     CDS             14279..15523
                     /locus_tag="ACHK01_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8096220.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGNGMHLLVHPNGSKYWRLQYRF
                     GGKQKMLALGVYPDVSLADARARRDDARKLLANGIDPGDKKKNDKIEQEEARTFEQLA
                     IEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKAVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASNNRQHRPALQLKLIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIEGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERSSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            16063..16914
                     /locus_tag="ACHK01_001242"
     CDS             16063..16914
                     /locus_tag="ACHK01_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096221.1"
                     /translation="MLVNSWCNALNEAISTSATVCAEDLYKGGHWATAKAILDDYPID
                     LWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYVGKSFHRTWWKEITNRSIF
                     KSKHPTSIVELMKKRKRDYYIICGSPDYINAIELDIINGLEYLVDAKKQLLIITSKKI
                     NGRLTTYLFKTNQNMAQWLRCNMLMLNISVAKYIVKEFTSKQLNDLNELSQKLKEELK
                     ELPEREVKKGIRRSPEEVKSFILKIMEKNPGISATHALREFRDSGNSFEEKRFRAEFM
                     ALREAKP"
     gene            16988..18238
                     /gene="dpdA"
                     /locus_tag="ACHK01_001243"
     CDS             16988..18238
                     /gene="dpdA"
                     /locus_tag="ACHK01_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HFO8096222.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLTECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYKNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRVHLLGIARPESFSDFIKFGVTSIDSTTPLQQAFKDRKNNYHTLD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMNALFEYDKGALSLEKALD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWKQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            18235..19365
                     /gene="dbpB"
                     /locus_tag="ACHK01_001244"
     CDS             18235..19365
                     /gene="dbpB"
                     /locus_tag="ACHK01_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein DpdB"
                     /protein_id="HFO8096223.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESANPMIPNPVIIAFDKRVRFEPLTDNDDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEINSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLTQRLNFGEGSLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEEKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSSGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDYLVTGYMKTVNADIK"
     gene            19802..22957
                     /gene="dpdE"
                     /locus_tag="ACHK01_001245"
     CDS             19802..22957
                     /gene="dpdE"
                     /locus_tag="ACHK01_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdE"
                     /protein_id="HFO8096224.1"
                     /translation="MPLEVGDLVDSPYGIGKLIRINNNDALIKYFDSPVNYEGIEQTV
                     EVKHLKKAELHAESLVYRYDYEVAHWQIARVVGAVPGGVRLHFPNKQQEDVSLNDIFV
                     RWDRPISDAAALLAERITYTPFWQDARLAYQHQMLKQRNACAGITSSLSSSIELEPYQ
                     LAVARRVLSDPIQRYLLADEVGLGKTIEAGLIVRQHVTENPLAHCVVIVAPGVLHRQW
                     QQELSARFHLEELLNKSIHIIDMAAFSTADPLSFRPDMLVIDEAHQVGAWAWDAEYEQ
                     RYASVNYLAQQSEKLLLLSATPVTGNEKNFLAMLHLLDAKNYPLTDTGLQKFQQRIQQ
                     REEIGSLFYAFNCDNDNMTLEENLEALTRMFPEDEVLNGMASQLLPHLDMMQPLRSEA
                     RDLLINKIRHYIGNRYRLHHRLLRNRRSNPAISVLMPGLSGLSLKKYATTNASNDMMI
                     AWRESAIMMHPQDPTLAAIYQLLFEAAIATPELLARLANYRLQQESLGAAQGFLYSDS
                     QLALLQHPLFDGEKEILLELIDRCKMECDSKRQRLIDVLEKLLDSNVRELPYIVVFCD
                     QPWVADQVFTDLMIPFIGKIQRHNPDKEVRFGKGSKCQILVCDRRAEEGVNLHGGRKI
                     ALHYDLLLSPNRMEQRLGRLNRYCATESATTILSMALVSEAEGLQNQWLTILNEVFDV
                     FEESIASLQYLIDEELQRLISNLYPLGEESMMQLSARLAGEDGRLTVEKQRIEMQEVL
                     LQIDGDIIEAREYADALAEIDENEEEFRRCARNLIGNVLKFDFIPDEKDPSIFRYRFN
                     PGDRGTGTLVSPQRLQKHCLLGFDLNQSSGSSPMTWPLSYYRADTSQGKIRPARLGEP
                     FHDAILQMLDFEERGVASAIIRITPGMGDENIFFRYDFLIEADVHTPALQRLADHLMP
                     PETITLWLDQAGQQVTNAEVLMILNEDYKPKDKGGRHLNLNEERWAQISHCISAKDWR
                     TWCNDSYSIAQRLAVEQFATQQALSLSRLEHYLSSAALHHANRQETGQMLRQGIAQPK
                     MTCLATRALIIASEAILDEDS"
     gene            22963..25530
                     /gene="dpdF"
                     /locus_tag="ACHK01_001246"
     CDS             22963..25530
                     /gene="dpdF"
                     /locus_tag="ACHK01_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdF"
                     /protein_id="HFO8096225.1"
                     /translation="MNLAQLIDNDFISSRLSECLLPEATAGHLDRRALIRDWLYREKH
                     AHHNDNELTVPDDSQPGWPRQEEWEASGINCQRLGQTFLLSAGPAWCPQWLEQGNGMP
                     FLASDQHQFCRQDTRVPMDYVLRRYFKKDNYISYQAPGQRDAVRRALFAPPGSTLLVN
                     LPTGTGKTLVAQALALTASAPGALCVLIAPTTALVLELASRFRKLLIEAGNKAESVTG
                     WYSGLPAQDKYDTYMRIRVGEQRLIVTSPEATCSSLQFALFEAAKQGGLRHVIVDEAH
                     IVASWGNDFRPHFQQLGGLIRGLKRVSREANLTPCSTLLMSATITEASRKILRDTFDQ
                     EMSELHACHLRPEPAYWCYRAKDHSDKRDKVLQSLAHAPRPLILYVTRPKEAEEWAQQ
                     LRLQGYQRLATFTGDTPSEVRRSLLEKWDKNQLDIMIATSAFGVGMDKNDVRTVIHAT
                     VPENMDRFYQEVGRGGRDGYACTSLLIYNHQDVKQAEHLSSQKLIGLELGLERWKKLW
                     GSAESVGDNLYRIDIERIHSGLNFRSKQNKLWNLRTVLMMVRAGVLELDADLQNPPDK
                     KDFADVNRYEAAKEQIIRQNGNSLVVRILRSHHTGEELWKTRIIPSREETLQAARANH
                     NALINWLHTPLSRPLCEALVDLYSMAEFIPGHACGGCPACRDNQALTSNYPSPPTLLL
                     NPPASVAQQALSRWQALSGLSYSSCFITYPPVNDDEWERENWYHRIATLLVQLYHRGI
                     VQRVWADPALLHSIFSAIPRGERLILLSDELPARDEPAPDNDVMAELAELVLPTQESC
                     ANGYLPPFNPAPLQLTLIPHDMPDPHHPGGAHYVLLDNHNWITIEQLQRKLDSVDYQ"
     gene            25514..26419
                     /gene="dpdG"
                     /locus_tag="ACHK01_001247"
     CDS             25514..26419
                     /gene="dpdG"
                     /locus_tag="ACHK01_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdG"
                     /protein_id="HFO8096226.1"
                     /translation="MSIINNAHGGSQYAVLAAFYQVLKASPKNQLPREKLMALCAPET
                     LQTGDSQNARGKASQELRAWLDLGLLTEEKVEETTQISFNANYFSTKSMPLRQAARRC
                     LQAQENNSDLSSRDERAVDLTLLAALLLALDVYQNAEIKSGNLEVLVNTFIPDFRINS
                     NEIAVVPGYLSWLGFAQQLTRDSYAIDPTDSIREELPFLMRVGEQLSIGEVLQRLNRA
                     LPVLDGGSYRQQVEERISLYGWHSLSPNRLSTSLSRALLRLQLSGVINMKAESDASGA
                     MQLTGRNGSVLRTVTHLTLNDVGVH"
     gene            26419..29475
                     /gene="dpdH"
                     /locus_tag="ACHK01_001248"
     CDS             26419..29475
                     /gene="dpdH"
                     /locus_tag="ACHK01_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdH"
                     /protein_id="HFO8096227.1"
                     /translation="MPQLQSYWPSEQNVTACILTEAESLADSQLLAVHEPMRLDRIEF
                     QSGKVTQVGEGALLDFLLEHNRPLPLIGASGVGKSHLVRWVHAQLKRREDHAKYHIIR
                     IPKNASLPRVLAAILEGLDGEEYQSIREKVNGVGKQLIPENVAEHIALKLRQALNAAY
                     TDAIEELRQEKMGKIQLEESRKKQLEEIQQHAASSRLPALFFDSVMTQYFTAPGACLH
                     NIAQRFCQGANDDSIANLRYEMSAEDFTFKGLDFRQVSPTVLPYLRNQQLLTSDEKKQ
                     AAARVVNVVRSQALEDTFVELFSFNRANFQDLMRMIRRQLLAEGRDLILLVEDLTATS
                     AIEDVLIACLIEEDEYDGKKVLCTLRSIIAVTEGHDSFKRLRNTLGTRARYEWVIQQH
                     ASENDVALKKRVIDFCGRYLNAARHGAAALETYHLQQDAQCYQEIPVWQDQEVLENES
                     AAPALASFGSSSIGHPLFPFNPAAIGQLVERHCKVKEQGLVFIPRNILHEILREPLKK
                     YRQNYLDGQFPPSQFAEIVCNPALQQRVRLEGLSQPERINSLLAVWGGNSTNLAGLTP
                     AICKEFGLPDAAVLLGGGGTPPEPAPEPVPEPAPVPESVPAPPSPLDEWKLVLDKWHQ
                     SKTLEQTRARDIRKWLLEALNARIDWSTELIQTSLMASSAKTQGRIHLPGVLVGNQSH
                     PLVKLEDTPELRSACLAMAHFNHYQSWDYDGSEIDYAAFHSFMDCIEHEVKLKVLQEE
                     REELSSIINDLQLSGAFLGLDGLSASHPATLVQALLTEPQVANSNHPLLDEFLTQARD
                     TVIDEHLKRRERLIALTAAKKPGAQPYAINGLMLVEASKKQSPDAGKQPGKIHEVKSK
                     LGGYRTLLLKTQESITERLSDGNSASLAAVNLCDTMKEIVELANSADVARPMGETVPV
                     LLKLLKSWKDPELKKLIRQLEKLTLPADNSHMLMREILAINGEQFEAFRQLLENWGKF
                     EDATDKYVQQRISDKGGDQVEDAENQVTDTLANLAEQLTLLEADLS"
     gene            29472..30263
                     /locus_tag="ACHK01_001249"
     CDS             29472..30263
                     /locus_tag="ACHK01_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096228.1"
                     /translation="MSEVNINDVVTPVASVPEERGIFARRSGDFKARLQRYQQMEKAQ
                     DIRDNLLRIAKKIDTPTQTLAQALCSYRVLRQVHDDETGKPIVGPFDRATFGNPQILL
                     TAFCQQYATHKHDVEQEGELALLTQEIRRLASELSTHTTAAWVNYIRGLKAQWEVDQR
                     LFSNLAHQEGQRKVQQHYIDLVEKFTSSSRGLPKTIEEFETVRDLHEKLCQQRGALKL
                     DVPEEVNHFLKAVAGQGATLDMLTPNVLSWLTEEDDPTRYRIRRL"
     gene            30279..34808
                     /gene="dpdJ"
                     /locus_tag="ACHK01_001250"
     CDS             30279..34808
                     /gene="dpdJ"
                     /locus_tag="ACHK01_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdJ"
                     /protein_id="HFO8096229.1"
                     /translation="MEQIIRAVLDGLESQEAVLLTWGNTQGFFTETELRNTISQVLQN
                     HPLDANGLTSFGIVKVMIDRGLLLRHQQNGVSVFRTRMAESVRLYSQLRQMFPKHGFN
                     DNWRTAPTLVSDYRFLRRQRKYPKRDRQAAEVLQQLDEIAGFTSTHRQLLSALLSHSS
                     GMYNLAYFQQEACKNVLRGLQQSQHRSTATIVCAGTGTGKTLAFYLPALTFIGSELVA
                     KRKDAVKALAIYPRNELLKDQFIETLRQTRKLNTELAKKGVRKVRIAALFGMVPTNKE
                     AIHRDYMKDAWCRHRNGMACLYIPCPTESCRGKMVWHTADYDKNLERLHCDSCSQTIE
                     PDEVILTRKRMKIELPDILFTSTEMLNKQMGNPFLASLFGIGKNVVPPSMMLLDEVHT
                     YSGVSGAQNGMLIRRWRHLSGATPHFVGLSATLEQATRFMASLTGLEEYLVAEVSPIE
                     DDMETEGAEYMLALRGDPASRSSLLSTTIQASMLTRRMLDNAAVPVSNGMYGTRSFVF
                     TDDIDVINRLYFQLLDAEGRYSNGNINAKKEPLAMLRGDAPNEEKFTFGQQWPLAKMI
                     GHTLDSADRSNVKRTSSQDAGVDHAADLIVATASLEVGFNDPNVGAVIQHKAPRDNAQ
                     FLQRKGRAGRQRTMRPWTVVVLSDYGRDRMAFQCYENLFEPVLKARQLPVGNSYVLRM
                     QAAFATMDWLSSRNEYLNQWNRGIWDDLSVPQDKGKPSEAQSKLADLIEDLLNSQKIQ
                     QEFNSWLAEALGIKDEKLLQSLLWQPPRAIMTAFLPTVLRRLRSNWSRLGIEQADNCR
                     KSTPMPDFIPSALFNDLCLPELQINLLGEVGQEPDAHSMPILQGMKDFAPGRISKRFA
                     IKNIRECHWLVPKKLELKDGSHPFPIDDYCPPDKRESMPDCHITTRTGMQVIPCFRAW
                     EVTASTPPDDLKLSETSNAFLNWHSEIRPPQNGIPAEVPSNNAWQDIFQQVEFYSHQQ
                     HCPIEAVRFATGSRANIKFSDQREDLQIDFKFEHQGEPAAFGFSLWVDAIKFQCRLPN
                     FDLASISNNRELVAGLRTARFLYEVSHDEAISVNTFLGGWLAETVLAGIGSEAVLKNC
                     TPEQAFRAMEHNSARIALIDVPARIFQTLYDAQTGEEIEQDLQQSLRELLSQAPVMAR
                     IADWVELLWQPLDNTWLEWLTLNFTNTLGAALLQTAMQLCPDADDNDLILDLNGGPVR
                     TSLLAPDQREIWLSETTVGGGGIIEKIQQIYREDPRSFFELLDFNLSPGDYETMDNNV
                     WHLLQTMVNPTSSLPACMNEMRLANDHASQVQAQRNLLGELQRSGFMTSHSFLSAINT
                     RLLRPGTDASSDVFLLQLQQDWRQQEQRLGIELPQRVYAFTCSQSSALDQQLQQASQG
                     QHNLESWRLNTCNGLLWPRGHTIRDSWLTWYNPYSFAGPTERLLLAHVVQHSCAQVAL
                     SQPDWMQQCHQHLENNGKCELLAEPGDAINEAIATLLVTPVEMYGLFFYPRVSALRRH
                     NNDTFAMIEIPEAIQ"
     gene            34805..35347
                     /gene="dpdK"
                     /locus_tag="ACHK01_001251"
     CDS             34805..35347
                     /gene="dpdK"
                     /locus_tag="ACHK01_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009114670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein
                     DpdK"
                     /protein_id="HFO8096230.1"
                     /translation="MNTRRIFKSRTSRQSEVLDVLSSLLVAEIAQPSAELWIVSPWIT
                     DLDLLDNRTGRFDYLEPAWGQRQVQTSELLARAVANGGTLKVMTNETQHNARFIRQLT
                     ERVANYGMLDNLYIGQKEVLHTKGFLGDHFFLSGSMNLTIGGIVINDEQINLTTDKTA
                     IVQALLAFNDFYQPAQGGVQ"
     gene            35344..37599
                     /gene="dpdD"
                     /locus_tag="ACHK01_001252"
     CDS             35344..37599
                     /gene="dpdD"
                     /locus_tag="ACHK01_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdD"
                     /protein_id="HFO8096231.1"
                     /translation="MNSADSEKFLRAFFHRENNIISWEKIAGGLLEASTQQRLTPWLD
                     DVRNGCFPVILPRVVKGSNDKVTWYAMADEERDYAELRELLDAHVGTSWSDFNRQRTM
                     LDPSDPVEKALIDHYSDRVYKFSSLSLSRIPEIFNGIERLRQQLASRPSYQLTLLRPR
                     GRILRDIRAALRRRDVPQTRLYLQELREAGGIGHINELFIELRCMALEGDAKSLLDST
                     QLRQVLDMPHPLVLAEEVMTVFYQQFLLKYEQLSDLAAQRQAFNNLLVKNVPRLFQVT
                     QGINTPSAIKNILLWLLSDTQSDPARLREQAQQVVAGMKGATDDDRHWIRRLLEAFPT
                     VAVASPPTASIDELLARADEYPESVLKELLLLPVSARGVAIALQCAWVVGSAGAAVSA
                     VSYLQQLAQQERDLLLAKPAFQRYYTALQLMLPSKRTEHDTAGINNWQDWLKEVLNNP
                     QWSGAIEAVEQACGQWNAAAFQPEAFVSLLDKTNNCASMVLRDAIPVLLEWLDNHQTS
                     ASWQSVRLALLQQLALDDNSSREDIFTACELARGYLEGDSNAHDYRELIENLQMMIES
                     SGTTSASAWLDLLELFLVYPTADQLAREQLFNLVLNMFYRNPARYSELQWSLLAQFAG
                     ECGLVCEIPPQILEDSGEKNDEVRVGSTLNDKVVGIYTLTESVAQRVKKQLLADYPQA
                     DIRLNNDKGGSTMLRSLAREADVFIFAWLSSKHSAYYAIKEERGDKPLLQPSGKGSTS
                     MLDCIRSYLKL"
     gene            complement(37656..38018)
                     /gene="queD"
                     /locus_tag="ACHK01_001253"
     CDS             complement(37656..38018)
                     /gene="queD"
                     /locus_tag="ACHK01_001253"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO8096232.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPEGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDIPGLENPTSEVLAKWIWNQVKTQLPIL
                     SAVMVKETCSAGCVYRGE"
     gene            complement(38318..38610)
                     /locus_tag="ACHK01_001254"
                     /pseudo
     CDS             complement(38318..38610)
                     /locus_tag="ACHK01_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            complement(38781..39356)
                     /locus_tag="ACHK01_001255"
     CDS             complement(38781..39356)
                     /locus_tag="ACHK01_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8096233.1"
                     /translation="MQNNQRWEPDWFLQSLLNNHIDKMVERYSCLRAFRTDLFYQDHT
                     AKFFLQDHQQLEWDIRKLMNRMMQLEAVVGFFWVIEWTEDHHYHAHVVFWLDGSKTQN
                     TYTWAQQAGLYWSDITDREGWYHRCEFKEHYEANINIPVRYSDPDSITNIRQVLAYMT
                     KIQQKHGLLLYGCNEVPARPLTGRPRSSQSL"
     gene            complement(39555..40148)
                     /locus_tag="ACHK01_001256"
     CDS             complement(39555..40148)
                     /locus_tag="ACHK01_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096234.1"
                     /translation="MKDYKPRYEELYRYYQVWLTDFTKLTVRSGMCHQNIYHHHTLTV
                     GSLKFPGEEEVIAIVPQCLHRIIYGRAAAPLTVNEDLSVSLFTQEHLLTHHPMLEGIL
                     LSECVRLKQRPLANKLISLFRQFSGSELRLKLVWLCWYDLMLGNCLDDWTDNLRFKKD
                     KEMDIWINNRQAENPALTIMMDEYVCFAWRTTAEPLS"
     gene            complement(40245..40451)
                     /locus_tag="ACHK01_001257"
     CDS             complement(40245..40451)
                     /locus_tag="ACHK01_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8096235.1"
                     /translation="MTTRPSLTEDQFVDMAFITSLLQMTDKWIYKLIKDGVFPKPIKL
                     GRSSRWLKSEVENWLQERIDQSRK"
     gene            40935..41819
                     /locus_tag="ACHK01_001258"
     CDS             40935..41819
                     /locus_tag="ACHK01_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096236.1"
                     /translation="MVKLVGNDSSKIKYLENKLIENGYHFYSGGVDKDYREYQLRVFN
                     YLVSQNVSEQNINSFFAEVDNSYTRGFPSESELDWYRNDPRASLWLSCELYEKLKEET
                     PKYNIDFLSPEALQPDHNVRIEAIRHCMDEWPMYFTTPAEFIKDKSIEWAELLDQHDL
                     FRSVRSSKVDVCSWLRDYLRGNTSIGLKRICGNSSEEIMSWCYASYFIWRKNNLHSPD
                     SVELFIRKFKSAWSTQKNRNKNKEEKKLVTMSVNISQQAHDMLRDMSMKDSMSNNAII
                     ESAILRLYNIKNSKVRSK"
     gene            41904..42779
                     /locus_tag="ACHK01_001259"
     CDS             41904..42779
                     /locus_tag="ACHK01_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8096237.1"
                     /translation="MNKPDGLQAFEQPLASLPCTLRQLILERIQNLTHYEPVIGIMGK
                     SGTGKSSLCNELFQGEVSPVSDVNACTRDVLRFRLRSGRHSLVIVDLPGVGENGRRDH
                     EYRALYRRMLPELDLVLWVIKADDRALTLDEQFWLGVMQPYRQKVLFVINQADKIEPC
                     YEWDTLTSKPSTHQQGNLKAKQAAIMAMFKPHHPVCAVSASTGWGIEDMVAAMMRCLP
                     DRATSPVVTQLHGRLCTEPVKSQARDSFGDAVGRVFDTAESSSLLPAPLKKVIRTVRD
                     AVVSVARAVWDWIFF"
     gene            43023..43739
                     /locus_tag="ACHK01_001260"
     CDS             43023..43739
                     /locus_tag="ACHK01_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8096238.1"
                     /translation="MTLQERRHDRLAVRLSLIISRLVSGETLFIRKLATEFGVSERTL
                     RRDFRERLMYLDLDYYQGQCSLRTDIHSTRQEIDILTFAHRTGLAGVFPGFDRRLVSS
                     LLDCDDRPCLVWHQDRPHPPAGKLSFYRLVRAISGCQRITILTEGERHEAVAPYRLIA
                     LDGYWYLVAEHNRQLRVFRLGEIHLVRPSRETFIRSELLYQLSEDKHFISALPHFRFI
                     QQSLNSFSPSESRGQSGQTD"
     gene            43775..44227
                     /locus_tag="ACHK01_001261"
     CDS             43775..44227
                     /locus_tag="ACHK01_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096239.1"
                     /translation="MKKLKLLSLLPVMFFPLLIAGNAHAARPYSGFLPAAVNNKSGNI
                     FWCDAHRQVRKLCTATEVDSMGWFIVVRSKPSRDCPGGIWGWITPSKDDVATGYTITP
                     GKEFNLTMKDMCSPDMKVTFRPNKKDPRYDDLVGIYAGKEAFRYKQIK"
     gene            44299..44772
                     /locus_tag="ACHK01_001262"
     CDS             44299..44772
                     /locus_tag="ACHK01_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096240.1"
                     /translation="MRLPYVIVTLAMLASVSTAQAIPNMWTSGFGMGVTEYIITSPDK
                     VVFNLNCTGNPDAQNILQHGVDLTLPDGISVSSHDDGTEITVVMDNSQYPLPSFLGWR
                     NGDNAWVSFIDALSQAANFDVYVNDKKVGTFSPGLKNTQKELSDLSECRTTHYSD"
     gene            44892..45713
                     /locus_tag="ACHK01_001263"
     CDS             44892..45713
                     /locus_tag="ACHK01_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8096241.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFEPFFACQTKVRDQSKREHTKHMLRLHRAGQLTGHQVPEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVEEKRDAMQSLALPAPARHALANAALEYRFGEDHQPVTVSQLLTPRRREDYSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVTGIDGDVKLNRALWVMAENMLEFFGR"
     gene            45744..46187
                     /locus_tag="ACHK01_001264"
     CDS             45744..46187
                     /locus_tag="ACHK01_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8096242.1"
                     /translation="MNTQNLEFAEQAAITASAVPDELRIGFWPQHFGSVPQWITLESC
                     IFDWMDRLCTDYHGGIWSFLTLSNGGAFMAPESEHDEKWALFNSMNGNGAELTGEAAG
                     IVACLMTYSHHACRTECDAMTEHYYRLRDFALNHPECSAIMHLID"
     gene            46200..46742
                     /gene="radC"
                     /locus_tag="ACHK01_001265"
     CDS             46200..46742
                     /gene="radC"
                     /locus_tag="ACHK01_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8096243.1"
                     /translation="MNTTISPLLSQPELFPVSVMAQHGCLLPTTSGLTPYAQRTIRRA
                     MNLLDKYLRQPGIAFTSSTAARDWLRLHLARQEREVFMVLYLDNQHRLLESETLFAGS
                     VNHVQVHSREVVKSALRFNAAAVVLAHNHPSGDPEPSQCDRNITGRLKEALELVDVKT
                     LDHLVVGRDGIVSFAERGWL"
     gene            46739..46960
                     /locus_tag="ACHK01_001266"
     CDS             46739..46960
                     /locus_tag="ACHK01_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8096244.1"
                     /translation="MKIISKRQAMALYRQHPGSRLFRFCTGKYKWSGSICHYAGREVQ
                     DIRGVLAVFAERRQDRNGPYVILRSITLN"
     gene            46986..47351
                     /locus_tag="ACHK01_001267"
     CDS             46986..47351
                     /locus_tag="ACHK01_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8096245.1"
                     /translation="MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLS
                     DRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGELNPRHPHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPEQTEPQ"
     gene            47390..47761
                     /locus_tag="ACHK01_001268"
     CDS             47390..47761
                     /locus_tag="ACHK01_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8096246.1"
                     /translation="MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFS
                     NETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDCSVMEKSPFITSIDILRARKASG
                     LMKRNSHKTVTRVTAGRLQEL"
     gene            complement(48561..48704)
                     /locus_tag="ACHK01_001269"
                     /pseudo
     CDS             complement(48561..48704)
                     /locus_tag="ACHK01_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            48786..48848
                     /locus_tag="ACHK01_001270"
                     /pseudo
     CDS             48786..48848
                     /locus_tag="ACHK01_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(48916..49896)
                     /gene="nanS"
                     /locus_tag="ACHK01_001271"
     CDS             complement(48916..49896)
                     /gene="nanS"
                     /locus_tag="ACHK01_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8096247.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWGEK"
     gene            complement(49961..51067)
                     /gene="nanM"
                     /locus_tag="ACHK01_001272"
     CDS             complement(49961..51067)
                     /gene="nanM"
                     /locus_tag="ACHK01_001272"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO8096248.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(51087..51803)
                     /gene="nanC"
                     /locus_tag="ACHK01_001273"
     CDS             complement(51087..51803)
                     /gene="nanC"
                     /locus_tag="ACHK01_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO8096249.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
BASE COUNT        12924 a        12798 c        13023 g        13122 t
ORIGIN      
        1 gaactattac tgcataaaat aatgtgatgc cgggatgttt ttgtatttcc cggcatcttt
       61 atagcgatat caattattta ctgagcgtcg cgaccatcac cgctttgatg gtatgcatgc
      121 ggttttccgc ctgatcaaaa acaatgctgg cggcagattc gaagacctca tcagtcactt
      181 ccattccgcc atgtaggcca aactcttccg ccattttctt accaagcgtc gtctggtcgt
      241 catgaaacgc gggcaggcag tggaggaatt tgacctccgg gttaccggtc aactgcatca
      301 tcttgctgtt cacctgataa tcacgcagta atgcaatccg ttctgcccat ttctcttttg
      361 cttcccccat cgacacccat acatcggtat agataaagtc agcaccttca actcctttgg
      421 ctacatcttc cgtcagcgta atattcccgc cattttgctg tgccagggcg cggcattccg
      481 taaccagcgc agcttccggc cagcatgcct gtggcgcgac cagacgcaaa tccagaccgg
      541 taagcgccgc agcttcgagc atcgaattgc ccatgttgtt acgtgcgtca cctgcataga
      601 ccagcgtcat ttcgttgaat gctttaccgg gtaaatgctc ctgcatggtg aggagatccg
      661 ccagcaactg cgtggggtga aactcattgg tcaggccgtt ccataccggc acgccagcgt
      721 attccgccag tgtttcgaca atctcctgac catagccgcg atactgaata ccgtcataca
      781 tgcgacccag cacgcgggca gtgtctttaa tcgactcttt atgaccaatc tgactgccgc
      841 ttgggccgag ataagtaacg cgagcaccct ggtcatatgc ggcaacttcg aaagagcatc
      901 gggtacgggt cgagtctttt tcgaagatga gcgcgatgtt tttaccagtg agtttggctt
      961 cttctttgcc gcttttctta tcggctttca gcttcgcggc taactgcagc aggctgttga
     1021 gttcagctgg cgtgaaatcg agtaatttca ggaaatgctt atgataaaac ccggacatag
     1081 atccctcctg tggctaacgc cttaatgaat taaaattcaa tttatatgga tgattattca
     1141 tttgcaagtc taaagcataa atctttgtca caaaggtgga ggcaatgtca gtggtgtgtg
     1201 acaataagag tatcggcagg acattaagag gaatgagcca tggcaaaccc ggaacaactg
     1261 gaagaacagc gtgaagaaac acgtttgatt attgaagaat tactggaaga tggcagcgat
     1321 ccggacgcgc tgtacaccat cgaacaccat ctttccgcag acgatctgga aaccctggaa
     1381 aaagcagcag ttgaagcgtt taaactcggt tacgaagtga ccgatccaga agagctggaa
     1441 gttgaagatg gtgatatcgt gatttgctgc gacatcctca gcgagtgcgc gttgaatgcc
     1501 gatctgatcg atgcccaggt tgaacaactg atgacgctgg cggagaaatt tgacgttgag
     1561 tacgacggtt ggggcactta ctttgaagat cccaatggcg aagacggcga cgatgaagat
     1621 tttgtcgatg aagacgatga cggggttcgc cactaattaa ttgattaaaa tgcccgatag
     1681 cacacactag cgggcatttt tttagagttc gcgtagcatc cgcacttcac aatctacatg
     1741 gcccgtgcag ccaagtgcat agtcgatatg ttgaaaacct aaatgttcat acaaaccaat
     1801 cgcttctttc aaaaaagcgg tcgtttccag atagcagcgt ttgaagccca tctctcgtgc
     1861 ctgctccatc gccattaagg ccagtttttt cgccagccct ttaccgcgaa tagccggaag
     1921 aaaatacatc ttctgcagtt cgcaaatgtc cgactgactc ccggttaatg gcgcaatccc
     1981 accgccgccg accacttcac catcgtactc aaccacccaa tatgcatgac ccggctgact
     2041 atacacttga tacaactcgt ccaggttcgg atcggcgacg gtgtagcctt tatcagcggt
     2101 aagaccgtat tcggcggata cctgacgaat gacgcgggcg atggcgggat tatcctgctg
     2161 cgtcaggcga cgcattacag gtgattgcgg cgcaatgtta ttcatagttt aacccaataa
     2221 aaataaagta attatacatt tgtttaatac cactctcttc ttacgtgcgc aagcgactta
     2281 taaagtaata aagtttttgc cagcctatta ctgataagag tattgtaacg tggcagaaca
     2341 ttttactgtc tgtatatata aattatatca atggattttt taattcaggg aatttttatg
     2401 gctcaagtta ttaatgaaat ggatgttccg tcccattcgt ttgtttttca tggtacaggt
     2461 gagagatatt ttcttatttg tgtggtgaat gtgttgttaa cgattataac gctaggtatc
     2521 tatttgccat gggcattaat gaaatgtaag cgttatctct atgctaatat ggaagttaac
     2581 ggacaacgat tttcttatgg aattactggt gggaatgttt ttgttagttg tcttgttttt
     2641 gttttttgct atttcgcaat cttaatgaca gtgtcagcag atatgccact tgttggctgt
     2701 gttttgactt tgttactgtt ggttttgctt atatttatgg cagcaaaagg actgcgttat
     2761 caggccttga tgaccagtct caacggcgta agatttagtt ttaattgctc tatgaaaggg
     2821 ttctggtggg taaccttttt cttgccgatt ttaatggcca ttgggatggg gactgttttc
     2881 tttatctcga caaagatgct acatgccaat agttcaagta gtgttattat atctgtggtt
     2941 ctgatggcaa tagttggtat tgtttccatt ggtattttta atggtacttt atatagcctg
     3001 gtaatgagtt ttctctggag caataccagt ttcggtatac atcgtttcaa ggtgaaatta
     3061 gatactacgt attgtataaa atatgccatt ctcgcatttt tagctttatt accttttctc
     3121 gctgttgctg gttatattat cttcgatcaa atattaaatg cgtatgatag ttctgtgtat
     3181 gcaaatgatg atattgagaa tttacagcaa tttatggaaa tgcaacgtaa aatgataatc
     3241 gcgcagttaa tctattattt tgggattgct gttagcacca gttatttaac ggtgtcgttg
     3301 cgaaatcatt ttatgagcaa cctgtcactg aatgatgggc gtattcgttt tcgctcaact
     3361 ttaacgtacc acggtatgct ttatcgcatg tgtgcgttgg tggtgatatc cgggattacg
     3421 ggcggtctgg cttatccact gctgaaaata tggatgattg actggcaggc aaaaaatacg
     3481 tatttgctgg gcgatttgga tgaccttcct ttaatcaata aagaagaaca accagataaa
     3541 ggcttcttag ccaggatttc acggggaatt atgccttctt taccatttct gtaagagata
     3601 aaaaaggccg gagcatgctc cggccttcgt tttcattact gtgttttgat tacagcgcgg
     3661 cgataacagc ctgctgttca atcagcttcg ctttcgcttc tgcatagcct tccagctttt
     3721 cacgctcttt cgcgatgacc gcttccggtg cgcgggcgac aaagccttca ttcgccagct
     3781 tgttctcgat acggctgatt tcaccttcaa tcttcgccac ttctttcgcc agacgcgcca
     3841 gctcatcttc tttgttgatg aggccagcca tcgggatcag cagctctgca ccgtcgatga
     3901 tcttagtaac ggaaaccgga cctttgtcat cggcaggcag cacggtgata ctttccagac
     3961 gcgccagcgt ttgcaggaag ccacggtttt catttacgcg acgttctgca tccgcgctgc
     4021 aaccacgcag tagcagctcc agcggtttgc ccggcgcgat gttcatttct gcacggatgt
     4081 tacgtaccgc aacgatcgcc tgcttcagcc actcggtgtc ggccagtgcg gcttcatcaa
     4141 cctgagatgc atcgtactgc gggaacggct gcagcatgat ggtgtcggca gtgataccgc
     4201 aaagtacttt cacacgctgc cagatggttt cggtgatgaa cggaatgatc ggatgcgcga
     4261 ggcgcagcag accttccagt acagtcacca gcgtatggcg agtaccgcgc agttctgctt
     4321 cggtgccacc gttcattacc ggcttggtca gctcgagata ccagtcacag aactggttcc
     4381 aggtgaactc atacagaatg cctgcggcga tatcgaagcg gaagctgtcc agcgcttcgc
     4441 ggtacgcttt gatggtctgg ttgaactccg ccagaatcca gcggtccgcc agcgacagcg
     4501 tcatttcgcc gccgttgaag ccgcaatcct gaccttctgt gttcatcagc acaaagcggc
     4561 tggcgttcca cagcttgtta cagaagttac ggtaaccttc caggcgcttc atatcccagt
     4621 tgatgtcacg accggtagac gccagtgccg ccagggtgaa gcgcagcgcg tcagtaccgt
     4681 gcggttcaat accgttcggg aactgcttct cggtgcgctt acggattttg tccgccagct
     4741 gtggctgcat catattgccg gtgcgttttt ccagcagttc tggcagcgaa ataccgtcaa
     4801 ccatatccag tgggtcaata acgttaccct tggatttgga catcttctgg ccttcgtcgt
     4861 cacgaatcag gccggtcatg taaacggtgt ggaacggcac ctgcggtttg ccattttcat
     4921 ctttgatgaa gtgcatggtc atcatgatca tgcgggcaat ccagaagaag atgatgtcga
     4981 agccggatac catcacgctg gttgggtgga actgacgcag ggcgtcggtg ttttccggcc
     5041 agcctagggt agagaaggtc cacagtgcag aagagaacca ggtgtcgaga acgtcttcgt
     5101 cctgacgcag ggcaacgtcg gcaccgaggt tattttcttt acgcacttcg tcttcgttgc
     5161 ggccaacata aacgttaccc gcttcgtcat accatgccgg gatacggtga ccccaccaca
     5221 actgacgaga gatacaccag tcctgaatat cgcgcatcca ggagaagtac atgttttcgt
     5281 actgcttcgg tacgaactga atgtcgccgt tctcaaccgc ttcaactgcc ggtttcgcca
     5341 ggacatcggc acgcacgtac cactggtcgg tcagcattgg ttcgatgacc acgccgccac
     5401 ggtcgccgta aggaacggtc aggtcatgtg gtttaatttc ttccagcagg ccgagtgcgt
     5461 caaccgcagc aacgaccgct ttacgtgcag caaaacgctc cagtttctgg aactctgcag
     5521 ggatttcgct ggaataaacg tcagattcgt tacctttggt atcgaacacc tgggcgcttt
     5581 cacggatatc gccgtcaaag gtcaggatgt tgatcatcgg cagggcgtga cgtttaccca
     5641 cttcatagtc gttaaagtcg tgcgccggag tgattttcac gcagccggtg cctttttcca
     5701 tgtcggcgtg ttcgtcgcca acgatcggaa tacgacggtt aaccagcggc agaatgacat
     5761 atttgccaat cagatctttg taacgcggat cttccgggtt aacggccacg ccggtatcgc
     5821 ccagcagggt ttccggacga gtagtcgcaa ccaccagata atctttacca tctgcggttt
     5881 tcgcgccgtc agccagcgga tagcggatgt gccacatcga acctttcgat tcgcggtttt
     5941 ccacttccag gtcagagata gcggtgcgca gtttcggatc ccagtttacc aggcgtttgc
     6001 cacggtaaat caggtcttct ttatacagac gaacgaaaac ttctttcacc gcattggaca
     6061 ggccttcgtc catggtgaag cgttcacgct cccagtccac ggagttgccg agacggcgca
     6121 tctgacgggt aatggtgccg ccggattccg ctttccattc ccagattttg tcgatgaaag
     6181 cttcgcggcc gtagtcgtga cgggttttac cttcttctgc ggcaatcttg cgctcaacga
     6241 ccatctgggt agcgatccct gcgtggtcag taccgacctg ccacagggtg tttttgccct
     6301 gcatgcgctg atagcggatc atggtatcca tgatggtttg ctggaaggcg tgacccatat
     6361 gcaaactgcc ggtgacgttc ggcggcggga tcatgatgca gaaactttcc tggctttcat
     6421 cgccattagg cttaaagtag ccctgctttt cccagtgctc gtaaagcggc tgttcgatat
     6481 cttgtgggtt atatgtcttt tccattattt ccaggttgcc gtattcaggt tgaaaccagc
     6541 cacgcggtag gctttatagc gttcgcgcgc cagttgtttc agagaatctt cataaggaac
     6601 gaagtctacc acttctgtga aagcggtggc aaaatctgca aagcttgttc gcagactaat
     6661 caatatatcg cgcgggctgc tgctgcgttt ttgcggccag gcgatctcca ccggcgcacc
     6721 gccgcgcggt ccttctcccg ctaaattatg cggaacaaag ctttctgccg gacgcgccca
     6781 cagggcttca tccagccggt aagcctgctt ttcatcttca caggcgatga gcacgcgctt
     6841 accactgcgc caacgttctg cggcaatttc acacaccagc tgctcaacgg cgcttaagcc
     6901 atcgacggtg gtgtcattgt ccagaaggta gaacgtcgcg tttttcataa atggggcttc
     6961 ttgtggtgga tttaaatatt gctgcattcc tcaccctaaa agatgagcaa tgaatttcta
     7021 ttgcgctcga tcggttccct ctcccttagg gagagggtta gggtgagggt aaaacgcaaa
     7081 cttactcttc gccgttaaac ccggcgcggt ttaacaggaa ctgtgccagc aacgctaccg
     7141 gacgaccggt tgcgcctttc gctttaccag aacgccaggc ggtaccggcg atatccaggt
     7201 gcgcccagtt gtacttacgg gtaaagcgtg acaggaagca acctgcggta atcgccccac
     7261 caggacgacc gccaatgttc gccatatcgg caaaattgga ctccagttgt tcctgatact
     7321 cgtcacccag cggtaagcgc catgcgcggt caccggattg ttcagacgcg gcaatcagtt
     7381 catgggccag cggattgtgg ttcgccatca gaccagtaat gtgatgaccc agcgcgatca
     7441 cgcaggcacc ggtcagcgtc gccacgtcaa tcaccgcttc cggctcaaaa cgctcaacgt
     7501 aagttaacac gtcgcacagt accaggcggc cttcagcgtc ggtgttcagc acttcaacgg
     7561 tttgaccgga catggtggtt aacacatcgc ccggacgata ggcacgcccg ccaggcatgt
     7621 tttcgcagcc agccagcacg ccgataacgt taatcggcag ttgtagctcc gcgaccatcc
     7681 gcatcacgcc gtaaaccgct gccgcaccgc acatatcgta cttcatctca tccatgcctt
     7741 ctgaaggctt gatcgagata ccgccggagt cgaaggttaa acctttaccc accagcacga
     7801 ttgggcgtgc atcttccgac gcgttgcctt tgtactcaat caccgacatc agcgattcgt
     7861 tctgcgaacc ctgaccgacc gccagatagg aatgcatccc cagctctttc atctgttgtt
     7921 cgccgataac gcgggtaatg acattcttgc tgtagctgtc agccagttgg cgcgcctgag
     7981 aggcgaggta agcggcgtta cagatattcg gcggcatatt gccgagatct tttgctgctt
     8041 taatcccggc ggcaatcgcc agaccgtgct ggatcgcgcg ctcaccgctg gtcagttcac
     8101 ggcgggtcgg cacgttgaac accattttac gcagcggacg acgcggttcg ctcttgttcg
     8161 ttttcagctg atcgaaactg tagagcgtct cttttgccgt ctcgacagcc tgacgcactt
     8221 tccagtagtt gttacggcct ttaacgtgca gttcagtcag aaagcagacc gcttccattg
     8281 agccagtatc attcagcgta ttaatggttt tctgaataac ctgcttgtac tgacgctcat
     8341 ccagctcacg ttctttgccg caaccaataa ggagaattcg ctcggaaagt acattcggaa
     8401 catggtgcag caacaatgtc tgccccggtt ttccttccag ttcgccccga cgtagcaggg
     8461 cgctgatgta cccatcgctg attttatcga gctgttctgc aatcggagaa aggcgacgtg
     8521 gttcgaagac gcccacgacg atgcaggcac tccgctgttt ctccgggcta ccgcttttta
     8581 cactaaactc catgcactac gctcctgaat cttaaagaca acggcggtgg ctacagatag
     8641 aattgcaagc tttcgtaact catgtccgct gttgcgatga cttcgtgtta atcttaacgt
     8701 tattacggca ttggcacgtc agaacaaaat ctgagaggtg aatccgttga gtataattat
     8761 cttagcgacg atttcgacga ctcaagagaa taaatgacgt ttaagccatg aaacaagcta
     8821 aaatcctgca aaagacgagt ttttacgggc gtatttaaag tgataatcat aagatatctg
     8881 gtgcgggaga cgctcaaaag ccagctggcg atactcttca tcttgctttt gatcttcttc
     8941 tgtcaaaagt tagtgaggat cctcggcgca gcggttgacg gcgatattcc ggcgaatctg
     9001 gtgctctccc ttctcgggtt gggcgtgccg gaaatggcgc agcttatcct gccattaagc
     9061 ctgttcctcg ggctgctgat gacgctgggc aaactgtata ccgaaagtga aattacggta
     9121 atgcatgcct gcggcctgag caaagcggtt ctggtgaaag cggcaatgat ccttgcggta
     9181 ttcacggcaa tcgtcgcggc ggttaacgtg atgtgggcgg gaccgtggtc atcgcgtcat
     9241 caggatgaag tgttagcaga agcgaaagcg aaccctggca tggcggcgct ggcgcaaggg
     9301 caattccagc aagcgactaa tggcagttcg gtgctgttca tcgaaagcgt tgacggcagc
     9361 gatttcaaag atgtgttcct cgcgcaaatt cgaccaaaag gtaatgcacg tccttctgtg
     9421 gtggtggccg attccggaca tttaacccag ctgcgcgacg gctcccaggt cgtcactctc
     9481 aaccagggaa cgcgcttcga aggcactgca ctgttacgtg atttccgcat tacggacttc
     9541 caggattatc aggcgatcat tggtcaccag gcggtggcgc tcgacccgaa cgataccgac
     9601 cagatggaca tgcgtacatt gtggaacact gacaccgatc gtgcccgcgc agaactgaac
     9661 tggcgtatca cgttggtatt caccgtgttt atgatggcac ttatggtcgt accgctgagc
     9721 gtggttaacc cacgtcaggg acgcgtactg tcgatgctgc cagccatgct gctgtatcta
     9781 cttttcttcc tgatccagac ctccctgaaa tcgaacggcg gtaaaggtaa gctggacccg
     9841 acgctgtgga tgtggaccgt taacctgatt tatctggctt tggcgattgt tctcaacctt
     9901 tgggacaccg tgccggtccg ccgcctgcgc gccagttttt cgcgtaaagg agcggtgtga
     9961 tgcaaccttt tggcgtactt gaccgctata tcggtaaaac tattttcacc accatcatga
    10021 tgacgctgtt catgctggtg tcgctgtcgg gcattatcaa atttgtcgat cagctgaaaa
    10081 aagccgggca ggggagttac gacgcgttag gcgcaggaat gtataccttg ctgagcgtgc
    10141 cgaaagatgt gcaaatcttc ttcccgatgg cggctctgct tggggcgttg cttggtcttg
    10201 ggatgctggc gcagcgcagc gaactggtgg tgatgcaggc ttctggtttt acccgtatgc
    10261 aggtggcgct gtcggtgatg aaaaccgcca ttccgctggt cttgctgacg atggcgattg
    10321 gcgaatgggt cgcgccgcag ggcgaacaga tggcgcgtaa ctaccgtgcg caggcgatgt
    10381 acggcggctc gttgctctct acccagcaag gcttatgggc gaaagatggc aacaacttcg
    10441 tctacattga gcgggttaaa ggtgacgaag agttaggtgg catcagcatt tatgccttta
    10501 acgagaatcg tcgtctgcaa tccgtacgct atgccgctac cgcgaagttt gacccggaac
    10561 ataaagtctg gcgtctgtcg caggttgatg aatctgattt gaccaatccg aaacagatta
    10621 ccggttcgca gacggtgagc ggcacctgga aaaccaacct cacgccagac aaactgggcg
    10681 tggtggcgct ggacccggat gcactctcaa tcagcggttt gcacaactac gtgaagtatc
    10741 tgaagtcgag tggtcaggat gccggacgtt atcagctcaa catgtggagc aaaatcttcc
    10801 agccgctatc cgtggcggtg atgatgctga tggcgctgtc gttcatcttt ggcccactgc
    10861 gtagcgtacc gatgggcgtg cgtgtggtca ccggtatcag cttcggtttt gtcttctacg
    10921 tactggacca gatcttcggc ccgctgacgt tggtttacgg catcccgccg attatcggcg
    10981 cactgttgcc aagcgccagc ttcttcttaa tcagcctgtg gctgttaatg agaaaatcgt
    11041 aaccaagcaa gggagcgaaa gctccctttc acattgttga taaagtcaaa tacgagttcg
    11101 atcggtcccc tcgccccttt ggggagaggg ttagggtgag ggggacagat cgcgcctgac
    11161 ttaccttctt ctccccccca gcaaactccc caacatcccg cgtacaatct gattcgtcac
    11221 ctggcgagcg gcgcttttcg ccattgtttg caccacacca tctttcttcc cgccgcgtgg
    11281 tccggtagtg ccaaacaaaa tatccttcaa tccaccaaga ataccgtcat ccaccgccac
    11341 ctctttccct ttcgtggggg gattcttttg ctgctcggta ctggcctgaa cgcctttttg
    11401 cagcatctca taggcggact cgcggtccac gtcatcctcg tacttgccat acaccgggga
    11461 gtgattaatc aagccattac gctcatcttc cgtcactggc cccattcgcg aacaaggcgc
    11521 gatcaccatc gctcgttcca ccactgaagg acttcctttt gcatcgagaa acgagattaa
    11581 cgcctcgccg gtccccagtt cctggattgc cttttcggta tcaaatgccg gattggcccg
    11641 catggtttgc gccgcagctt ttaccgcttt ctgatctttg ggcgtaaaag cccgcaaagc
    11701 gtgctgaacg cgattaccga gctggccgag cacattatcc ggaatatcag acgggttttg
    11761 cgaaacgaac cagacgccca cgccttttga gcgaataagc cttatcacct gctcaatctt
    11821 atccagcagt acctgcgggg catcgttaaa cagcagatgt gcttcgtcga agaaaaacac
    11881 cagttttggt ttctccagat cgcccgcttc cggcaattgt tcatacaact ctgaaagcat
    11941 ccacagcagg ctggcggcgt acagtttcgg catctgataa agcttctcgg cgctgaggat
    12001 attgataacg cctttaccgt tggtatcggt gcgcatccag tctttgatat ccagcatcgg
    12061 ctcaccaaag aagtgtgctg cgccttgctg ttccagcgac aatagcccgc gctggatggc
    12121 accaaccgat gcgctactga tatttccgta ctgattctgg aaggatttgg cgttatcgcc
    12181 gatgtactgg gtaatcgccc gcaaatcttt aaagtcgagc agtaacagcc cctgatcatc
    12241 agcaatgcgg aagatgatat tcagcacgcc agattgcaca tcgttgagat tcaacagccg
    12301 cgccagcaac agcggcccca gatccgaaac cgtcgcccgt accgaatggc ctttctcgcc
    12361 aaagatatcc cacaccacca ccggattggc atgcggttgc cagtcattga cgccgatatt
    12421 tttaagcctt gtgagcagtt tttccgacgc cgttcctgcc tgcgcgatac cggtcagatc
    12481 gcctttcaca tcagccataa acaccggcac gccgatttcc gacaatgact ctgccagttt
    12541 ttgcagcgta acggttttac ccgtccccgt tgcgccagta atcagcccgt gacggttagc
    12601 cattcccggc agtaaaaaca gttctgtgtc tggcgtgcgg gcaattaaca ggggttcgct
    12661 catgagattt cctccagttt cctgcctgga gtataggcaa cggctgcgca ctcttcaccg
    12721 ctaaattcag cagattgcga gatgccttcc aaagagattt tagtgccggg cgacaggcac
    12781 ccggcaaagg gatcagaagt cggctttcag aaccacgcgg taacgcgcct taccgtcgcg
    12841 cacatgcttg atggcgtcgt taattttcga catcgggaac aattcggtgg tcggtgcaac
    12901 cttgctgcgg gcggcaaagc gcatcagctt acgcagctca taaggcgtgc cggtagcaga
    12961 accagagatg ctgcggtcgc ccgcaattaa cgtaaaggcc ggaacagaca gcggcgtgag
    13021 aaccgcaccg accgtatgga aattaccgcc ataggtcagc gcctcaaaat aaggctgcca
    13081 gtcgaggctg acgttgacgg tgttgataat gagatcaaac tgccccgcca gtgctttgag
    13141 tgcctgcgga tcgcggctat tcaccacttt atcggcaccc atcgccagta cttcctgctc
    13201 tttcgccgga ttcgaactaa aggctgtcac ctcgcatccc attgcgtgca gaagttttat
    13261 agcgatatgc cccagcccgc caataccaat taccccaacg cggctggtgg ctgtgatatg
    13321 gtgcatcaac agtggtttaa agaccgtgat accaccacac agcagcggcc cggcggactc
    13381 gatatcaata ttttctggca gtggaatcac ccattgccag tctgcacgca acttctcggc
    13441 aaaaccgccg cgattcataa tcgtcggcac cgcgccttgc tcgcagttga tctgattacc
    13501 gctaatacag gcgtcgcagt gaccacagct acgcgccgtc cagccaattc cgacacgctg
    13561 accgacctgc aaacctttat cctgcgcggc gctcccgagc gccaccacgc gcccaattac
    13621 ctcatgcccg gcaaccagcg gatattgtga aaatccccat tcgttatcga tcattgacag
    13681 atcggaatga cagatcccgc agtaatccac ctgcacttca acatcttgtg gcttcagctc
    13741 accgggatcg tactcataaa cttccagttc gccgcccgct tcttttgcgg catagctttt
    13801 tatcatcgac attttttggt ccttctctgg tgttgtctgg gaagtgtaga gcatggcagg
    13861 gcggggtgcc tggagtgtga caaaggttac acatcgctgt atgcaatgct gaaaatttca
    13921 gcacttagcg aggtgcgagc aagctggcgc ttgcatggtg gcgtgcgaca ggtataatcc
    13981 acaacgtttt ccgcatacct cttcagtgcc gaagtggcga aatcggtaga cgcagttgat
    14041 tcaaaatcaa ccgtagaaat acgtgccggt tcgagtccgg ccttcggcac catcggaaca
    14101 tcaatagacg tcaacggacg tctttttttg tgcctgaaat ccagtattta cccatcattc
    14161 ccgctatatt aactctctca aggtcaaccg acttcaacgt gcatctacca acagatgttg
    14221 gtacagatga tggtatttcc ggttcgataa tgcttgtacc aacagggagg ggataagcat
    14281 ggctctaaca gatatcaaag tcagaacagc caagccaacg gataagcaat ataagctgac
    14341 cgatggcaac ggtatgcacc ttcttgttca tccaaatggc tctaagtact ggcgtctgca
    14401 gtaccgattt ggcggaaagc aaaagatgct ggctctgggt gtttaccctg acgtatctct
    14461 tgcagatgcc agagctcgtc gcgatgacgc tcgtaagctt ttagcaaacg gcatcgatcc
    14521 gggagataaa aagaaaaatg ataagattga gcaggaggaa gcacgtactt ttgagcagct
    14581 tgctattgag tggcatgcca caaataaaaa gtggtcggaa gaacatagtc gacgagtgct
    14641 taaaagcctg gaggacaatc tcttccctgc cataggtaag cggaatatcg ctgatcttaa
    14701 aactagagac ctgctggccc caatcaaagc ggtagaactg tcaggacggc ttgaggttgc
    14761 ttcacgatta caacagcgaa ccacggcaat tatgcgctat gccgtacaga gtggtttact
    14821 ggactataac cccgcgcagg agatggttgg cgctgttgct tcaaataaca ggcagcatcg
    14881 acctgctctt cagttgaagc taatcccaga actattacgg cgcatagata gttataccgg
    14941 tagacctctt actagacttg ccgtcgaact cacgctcctg gtattcattc gttcaagtga
    15001 actacgcttt gctcgttggt ctgagatcga tttcgaaaca tcaatgtgga caatacctgc
    15061 agagcgcgaa gctattgaag gagtaaagca ttcacagcgt ggttcaaaaa tgcgtacgcc
    15121 tcatctggtg ccgctttctc atcaagcttt agcaattctt aaagatgttt acaaacttag
    15181 tggtgatcgc gattttgttt tcatcggcga tcatgaccat cgcaaaccga tgagtgaaaa
    15241 cacggtgaac aaggcgctgc gcgttatggg ttatgacacg aaggtagagg tttgtgggca
    15301 cggtttcagg acaatggcct gtagttcatt gatcgagtct ggattgtggt cgagggatgc
    15361 agtggaaagg cagatgagcc atatggaacg tagctcagta cgcgcagctt acattcataa
    15421 ggccgaacat cttgatgaac ggcgactgat gctgcagtgg tgggctgatt ttttggatgc
    15481 gaatagggaa agcgaagtta gcccttatga ctttggcaag tagtctaatt cctgatgctt
    15541 gctatgttcg aatttgaaaa gccggcttga actataaccc atactttttt ataactgtat
    15601 tattctggcg actcagaggt gcactatgaa aactttcgat actcaacttg cgatgagcat
    15661 tgtatcgcac tcaacgggag gttttagtta tgacacactc gctagggcat tgatttctaa
    15721 agataaaaac gaaaattctg acgtccttct tgagtcatgt cgaaattttt gtctctctct
    15781 tgaggaaaaa ggtattctga ggcgatgcca aagatgttct cattctcaag atgagtattt
    15841 cgagtatatt cctcactaac atcagtccct gaaaattttt tcagggactg attactccgt
    15901 tcttgtcatc cccttttttc tcctgtagct tgtgtaacac gattctgggg ggcgattgtg
    15961 agcaacatca acttaataac cacatgtaca aacggcaaac actacggtag tagggatata
    16021 ttgagcctga gtcaattttc atcaggccga accccttcaa atgtgctcgt gaattcttgg
    16081 tgcaatgctc tgaatgaagc tatttctaca tctgcaactg tatgtgcaga ggatctttat
    16141 aaaggtgggc actgggcgac ggctaaagcc atcttagatg attatcctat tgatttatgg
    16201 attctttctg ctggtttagg acttcttcac cacaaagaca atgttgtacc ttacaaagcg
    16261 acctttgctg ttggatatga cgaatcaata ccattgtatt ctcaagaata tgtagggaaa
    16321 agttttcata gaacatggtg gaaagaaata accaatagat ctatttttaa atctaagcat
    16381 cctacatcta ttgtagaatt aatgaaaaaa agaaaaagag actattatat tatatgtggc
    16441 tctccagatt atattaatgc aattgaacta gatatcatta atggtcttga gtatctagtt
    16501 gatgcaaaaa aacaactgct tatcataact tccaaaaaaa tcaatggtag gttaactaca
    16561 taccttttca aaaccaacca aaatatggct cagtggctga gatgcaatat gctcatgctt
    16621 aatatcagtg tggcaaaata cattgtgaag gaatttacgt caaaacaact caatgattta
    16681 aatgagttat ctcaaaagtt aaaagaagaa ctcaaggagt tacctgagcg tgaagtgaaa
    16741 aaaggaattc gtcgtagtcc ggaggaagtt aagtctttca ttttaaagat tatggaaaaa
    16801 aatcctggta tcagtgcaac acacgcgttg cgtgaatttc gtgactcggg gaattcattt
    16861 gaggaaaaac gttttcgggc tgagtttatg gccctgagag aggctaaacc ttaatgctac
    16921 ttgcctgggt ttcatggtag aatcccccgt tttttgaggc tttgcagtgg cgtcacagga
    16981 gagtaagttg tcgaagctaa agtatttttt ccctgatagt caggatttta tcgatcccag
    17041 tttcgacttc tttcgcgaaa cgcgtaacga acatcgcgtg cgtcaacgtg acgatcagta
    17101 tccgcatgag gtttttcctc atccttacga tggtatgctg gtttctaaag ctgtagttga
    17161 tggtcttggc ggcggcgaga gcaaatatac tcgtgcccag cgtttgcgtt acttccgtaa
    17221 tggaatgaag cactttttcc gtctgccgga aaatatggaa actatgggtg attgcggagc
    17281 cttcacctat gttaatcagg acgtcccgcc ataccgtgtg gaagaggtga ttgagtttta
    17341 cgaaacttcc cgctttaacc atggcgtttc tctcgatcat atcgtttttg gctatgaaaa
    17401 acctggtgag gtatttagtg gcgaagtatt gactgagtgt cgccgacgcc aggatatcac
    17461 gctgaccttg gctcaagagt ttttaaataa gtctcaaaag tcctgtttca ccccctttgg
    17521 cgttgcacat ggatggagca aaacctccta tcgtcagtct gtagaggccc tgctggccat
    17581 gggatacaaa aatatcacta tgggggggat ggtgccgttg aaaacggcgc agatcctaga
    17641 gactctcgaa gagattaagc cgttgctgaa aagtgatacg cgtgttcact tgcttggtat
    17701 tgcccgtcca gagagtttct ccgactttat caagtttggt gtgaccagta ttgactcgac
    17761 tacaccgctt cagcaggcct tcaaagatcg aaaaaacaac tatcatacgc ttgatggccc
    17821 agcctatacc gctgtgcgtg tccctcagtt tgatgcgaat ccaagtttga gtcgcaaaat
    17881 taaatctggc gtgattgatc aggatattgc acggcatctg gaaaaggatg ccatgaatgc
    17941 cctgttcgaa tacgataaag gggctttgtc ccttgagaag gctcttgatg cagtcctggc
    18001 ttatgaacgt ctgcattcgg gtgaaaaaga ggccgacaaa attcgtgcgg attatgaacg
    18061 tacgctagga gaacgaccct ggaaacaatg ccagtgcaat atttgccagg ccatcggtat
    18121 caatgtcatc attttccgcg gagcagaacg taaccgtcgc cgcggtttcc ataatattca
    18181 ggtgttgtac aaccgtttac agcacacatt gtcattacgc tcagaggaac tgtcatgagt
    18241 gaatatcgtg ttcctgccct gcgaatccga cagggtgagg agaggcaact ctatagcctt
    18301 gcgatagaag gtaaacagat cagcaaaatt gcagcgattt cgcgtattcg tcgcggcgaa
    18361 gagagtttgg ttggttatca gcgtccagaa gtacgtaacc atattcgcga aattcagcgc
    18421 tacattgaga gcgcaaatcc gatgatacca aacccggtga ttatcgcttt tgataagcgt
    18481 gtgcgttttg aaccgcttac agataatgac gacatgggac atctggtaat acctttctcc
    18541 gaagataagg attttgagaa gccaggtttt attgtggatg gtcaacaacg tactgctgct
    18601 ttgcgggatg cagaaatcaa ttcgtttatg atgccggtct ccgcttttat cgctaatgac
    18661 gcggaagaac agcgtgaaca gttcatgctg gttaactcca caaaaccgtt gcccaaaacg
    18721 ttgttatacg aactggcacc gcatacccac ggtcgtttgc catccgatct gcaaatgcgt
    18781 aagtttcctt cactgctgac tcaacgactg aacttcggcg aaggttcact tgcagggcgt
    18841 atcaaaacgg cgaccaatcc tgatggtgtt attgctgaca actcgatgat caaaatgatc
    18901 gatgccagtc ttcgcgaggg agcattgtat cgcttccgtg acccggcgac agggttgggg
    18961 gatgaagaga agatggtgaa actgctgaat aacttctggt cggctgtgga aactgtgttt
    19021 actgatgact gggataaaaa gcctcgctat tcccgcttgt tacatggcgt tggtattctt
    19081 gcacttggca gcttgatgga tgaaatcgat caggtccatc aggactataa aggtgaacct
    19141 ggctggactg agatcccttc ctacactcgt tttgtcgaag agctaaaccg aataaagccg
    19201 ctttgtgcat ggagcagtgg cgtatggaac ttcggcacag atattgatgg tcagcctatc
    19261 gtccgtaaat ggaacgaact gcaaaatctt tcaaaagata tatctttggt tactgactat
    19321 ttggtaactg gctatatgaa aactgtcaat gcagatatta aataagggtg acaacccaat
    19381 tggcattaga agtatcgagt gctgattatc gacgtggggg gctgcagata tggtgcagaa
    19441 gttcaaattc tgtgcaagcg ccagacagcc cccatgcttt cattcttatc atcaaacgct
    19501 gaccttgtta cgatatatgt tcgtaactca tgaaaaatca agagtttttt gttgaaattc
    19561 agtcgtttca cgagttttga aagccggtaa ataagtcata ggccgcaccg gattacccac
    19621 caatcccccc taaaagtcat agtagactag taaccgtgat ccccctcctt gacaatcatc
    19681 tttacgggtt gcagtgtatg acattgtcct tgtattcagt gatattatac gtataaaaaa
    19741 tcaccattaa tcaaaggctt ttaaaatcac tgttacgcac agcaaattta tggatggata
    19801 tatgcccctt gaagttggcg acctcgttga ctcgccttat ggaatcggca aacttattcg
    19861 cattaacaat aatgatgctc taattaagta tttcgattct cccgtaaatt atgaaggcat
    19921 agagcaaact gtcgaagtaa aacacctaaa aaaagccgaa ctccacgcgg aaagtctggt
    19981 ttatcgctac gactacgagg ttgcacactg gcagattgca cgtgttgttg gtgccgtgcc
    20041 tggaggggta cgtttgcatt ttcctaacaa gcagcaggaa gacgtcagct taaatgatat
    20101 ctttgtgcgc tgggatcgcc ctatcagcga tgctgcggcc cttctggccg agcgcatcac
    20161 ctataccccc ttctggcaag atgcccgtct agcttatcag catcagatgc tgaaacaacg
    20221 caacgcttgt gctgggatta cctcttcgct gtcgtccagt attgaactcg aaccttacca
    20281 gttggccgtc gcgcgtcggg tgttatctga ccctattcaa cgctatctgt tggctgatga
    20341 agtcggcctc gggaaaacca tcgaagctgg gctgatcgtg cgtcaacacg tcacggaaaa
    20401 tccgctcgcc cactgcgtgg tgattgtggc gcctggagtt ctgcaccggc agtggcagca
    20461 ggaactttct gcacgttttc acctggagga actgcttaat aagtcgatcc atatcattga
    20521 tatggccgcc ttcagcacag cggaccccct gtcgttccgc cccgatatgc tggtgattga
    20581 cgaagctcat caggtcggtg cctgggcatg ggatgcagag tacgagcagc gctacgctag
    20641 tgtgaactac ttggcgcaac aaagtgaaaa attgctatta ctatcagcga cacccgtaac
    20701 tggcaatgaa aagaacttcc tggccatgct gcatctgctg gatgctaaaa attacccact
    20761 gactgacact ggcttacaga aatttcagca gcgtatccag cagcgcgaag aaataggttc
    20821 actgttctac gcgtttaact gtgataacga caacatgacg ctggaagaga acctggaagc
    20881 gttgactagg atgtttcctg aggatgaagt actaaacggg atggcttcac agttgcttcc
    20941 ccatttagat atgatgcaac cgctgcggag tgaggcgcgt gatctgctga tcaataaaat
    21001 ccgccactac attggtaacc gttaccgtct gcaccaccgc ctgctacgca accgccgtag
    21061 caaccccgcc attagtgtcc tgatgcctgg cttgtccggc ctatcactaa aaaaatatgc
    21121 cacgacgaac gccagcaacg acatgatgat cgcctggcgc gaatcggcaa taatgatgca
    21181 cccacaagac cccacgttgg cggcaattta tcagttactg tttgaagccg ctatcgctac
    21241 gccggaacta ctggcccggc ttgcaaacta tcgcttacag caggagtcac ttggtgccgc
    21301 gcagggcttt ctctatagtg attcacagct cgcgctgttg cagcatccgc tgtttgatgg
    21361 cgagaaggag atcctgcttg agctgataga ccgctgcaaa atggagtgtg acagcaagcg
    21421 ccagcgctta attgatgtat tggaaaaact tctggatagt aacgtcagag aactccctta
    21481 tattgtggtg ttctgtgacc agccatgggt agccgaccag gtatttaccg acctgatgat
    21541 accatttatt ggcaaaatcc agcggcataa ccccgacaaa gaagtgcgct ttggcaaggg
    21601 aagcaaatgc cagatcttag tgtgcgatcg ccgggctgaa gaaggtgtca atctgcacgg
    21661 cgggcgcaag attgcgctgc attacgactt gttgctgtcg cctaaccgta tggaacagcg
    21721 tcttggccgc ttgaatcgct attgcgcgac tgagagtgcg actactatcc tcagcatggc
    21781 gctggtgagt gaagcagagg gtctgcaaaa tcagtggctg actattctca atgaagtgtt
    21841 cgatgtcttc gaagagtcga ttgcaagcct acaatacttg attgatgagg aattacagcg
    21901 gcttatttcc aatctttacc cgctgggtga agagagcatg atgcaactca gtgcgcgtct
    21961 ggctggtgag gatggccgcc ttacggtgga aaagcagcgc atcgaaatgc aggaagtcct
    22021 gctgcaaatt gatggcgata tcatcgaagc cagagagtat gccgatgcac tggcggaaat
    22081 agatgagaat gaagaggaat tccgccgctg cgctcgtaat ctgataggta atgtactgaa
    22141 attcgatttt attccagatg agaaagatcc atcgatattc cgctatcgct tcaaccctgg
    22201 tgatagaggg acgggcacct tagtcagccc acagcgccta caaaaacact gcctgctggg
    22261 ctttgatctc aaccaatcta gcggaagttc gcccatgact tggccactta gctactaccg
    22321 ggctgatacc tctcagggta aaatccgccc ggctcgcttg ggtgaacctt tccatgatgc
    22381 catcctacaa atgctggatt ttgaagaacg cggtgttgct agcgcgatta tccgtatcac
    22441 accaggcatg ggcgatgaaa atattttttt ccgctacgat tttctgattg aagctgatgt
    22501 ccatacacca gcactgcaac gactggcgga ccatctgatg cctcctgaaa ccattacctt
    22561 gtggctggac caggccggcc agcaggtcac caacgcagag gtgttgatga tacttaatga
    22621 agactataaa ccgaaggata agggcggacg ccacctaaat ctcaatgagg aacgctgggc
    22681 gcagatctcc cattgcatca gcgctaaaga ttggcgcacc tggtgtaacg atagctatag
    22741 catagcccag cgcctggcgg tggaacagtt cgccacacaa caggctctca gcctgagccg
    22801 actggaacat tatctaagca gcgccgcgct gcatcatgct aaccggcagg agacagggca
    22861 gatgctgcgc cagggtattg cacagccgaa aatgacctgc ctggcgacgc gtgccttaat
    22921 cattgcatca gaagctatat tggatgagga ctcataaacc ggatgaattt ggcgcaactg
    22981 attgataatg attttatttc atcaaggttg agtgagtgcc tgctgccaga ggcgactgcc
    23041 ggacacctcg accgccgggc gttgatccgt gactggctgt accgggaaaa acatgcccac
    23101 cacaacgata acgagctgac cgtccctgat gatagccaac ctggttggcc aagacaggag
    23161 gagtgggaag ccagcggtat taactgtcaa cgactaggtc agacctttct gctcagcgct
    23221 ggccctgctt ggtgtccgca gtggctggag cagggaaacg gcatgccttt tctcgccagc
    23281 gatcagcacc aattctgccg ccaggacact cgagtaccga tggactatgt cctgcgccgt
    23341 tacttcaaga aggacaacta tatcagctat caggcaccgg gccagcgcga tgcggtacgc
    23401 cgggctctgt tcgccccacc gggcagtacg ctgttggtca atttgcccac aggcaccggg
    23461 aaaacactgg tagcgcaagc attagctctg acagcctcag ctcccggggc actttgtgta
    23521 ctgatcgccc cgaccacggc gctggtgttg gagctggcta gccgtttccg gaagttactg
    23581 attgaagcag ggaacaaagc agaatctgtc accggctggt atagcggctt accggcgcag
    23641 gataaatatg atacgtacat gcgtatccgt gtgggtgagc agcggctgat cgtcacctct
    23701 cctgaagcga cctgcagttc cctgcagttc gccttatttg aagcagcaaa acagggaggg
    23761 ctgcgccatg ttattgttga tgaagcgcat attgtcgcca gctgggggaa cgactttcgc
    23821 ccgcatttcc agcaacttgg tggcttaatt cgtgggctga agcgcgtcag ccgtgaagcg
    23881 aatcttacac cgtgctcaac actgctgatg agtgcgacta tcaccgaagc cagccgtaaa
    23941 atcctgcgcg ataccttcga tcaggagatg agcgaactcc atgcctgcca tctgcgcccg
    24001 gaacctgctt actggtgtta tcgggcaaaa gaccacagcg acaaacggga taaggtgctg
    24061 caatcgctgg ctcatgcgcc acgcccgctg atcctctacg tgacgcgacc gaaagaagct
    24121 gaagagtggg cgcaacagtt gcgtttgcag gggtatcagc gtttggcaac atttaccggt
    24181 gatacaccga gtgaagtacg ccgaagcctg ctggaaaaat gggataaaaa ccagctggat
    24241 atcatgattg cgacctcagc atttggcgta ggtatggata aaaacgacgt gcgcacagtg
    24301 atccatgcca cagtgccgga aaacatggac cgcttctacc aggaagtggg acgtggtgga
    24361 cgcgatggct atgcctgcac ctcactgcta atttacaatc atcaggatgt caaacaggca
    24421 gaacatctgt cctcacaaaa gttgattggt cttgagttgg gcctggaacg ctggaaaaag
    24481 ttatggggca gtgccgaatc ggtgggcgac aacctgtatc gtattgatat agagaggatc
    24541 catagcggcc ttaatttccg cagcaaacaa aataaattat ggaacttgcg taccgtactg
    24601 atgatggtgc gtgcaggagt gctggaactg gatgccgatc tacaaaaccc gccagacaaa
    24661 aaagactttg ccgatgttaa tcgctacgaa gcggcaaaag agcaaatcat ccgacagaac
    24721 ggaaattcac tggtagtacg catcctgcgt agtcaccaca ccggagaaga attgtggaaa
    24781 acacgtatta tcccttcccg tgaagagacg ctgcaagctg caagggctaa ccataatgca
    24841 ttaatcaact ggctgcatac gccactgagc agaccgctgt gtgaggcact tgtagatctc
    24901 tactccatgg cggaatttat tccgggtcac gcctgtggtg gttgccctgc ctgtcgcgat
    24961 aatcaggcgc ttactagtaa ttatccgagc ccaccgacgc tgttgcttaa cccgccagcg
    25021 tcggtggcac agcaggcgtt aagccgctgg caggcgctca gcggcctatc ttactccagt
    25081 tgctttatca cctatccgcc agttaacgac gatgagtggg aacgtgagaa ctggtatcac
    25141 cgcatcgcga cgctactggt gcaactgtat catagaggca ttgtccagcg cgtctgggca
    25201 gatcctgcct tgttgcatag tatatttagc gccattccgc gcggtgagcg cctgatcttg
    25261 ttgagtgacg aattgcctgc acgtgatgag ccagcgccgg ataacgatgt aatggccgag
    25321 ttggcagagt tggtacttcc cactcaagag tcctgcgcca atggttacct gccgcctttc
    25381 aacccagcac cattacaact gacgctgatc ccgcatgata tgcccgatcc tcaccatcct
    25441 ggcggggcgc attacgttct tctcgataat cacaattgga tcaccatcga acaacttcaa
    25501 aggaaactag acagtgtcga ttatcaataa tgcccatggt ggcagccagt atgccgtgct
    25561 ggccgcattt tatcaggtgc tgaaagccag cccgaagaac caactaccgc gtgaaaagct
    25621 gatggcgctc tgtgcaccgg aaacattgca aaccggcgac tcacaaaatg cacgtggtaa
    25681 agcgtctcag gaattaagag catggctgga cttggggtta ctgacggaag aaaaagtaga
    25741 agagacaaca caaatcagtt tcaacgcgaa ctatttcagc acaaaaagta tgccgctgcg
    25801 ccaggctgcg cgccgctgtt tacaggcaca ggaaaataac agtgacctga gctcacgcga
    25861 tgaacgcgcc gtcgacctta cccttttggc tgcattgctg ctggcgctgg atgtctatca
    25921 gaacgcagaa atcaaatcgg gcaaccttga agtgctggtg aacacgttta tacctgattt
    25981 tcgtatcaac agtaatgaaa ttgctgtcgt tccggggtat ctcagttggc tgggctttgc
    26041 tcaacaactc actcgcgaca gttacgccat tgaccccacc gattctattc gtgaagagtt
    26101 accgttcctt atgcgtgttg gcgaacaact ttccatcggc gaagtgctgc aacgtcttaa
    26161 tcgggcgctc ccggtgctag atggtggcag ttatcgccag caggtcgagg agcgtatcag
    26221 tctgtatggc tggcactctc ttagccctaa ccgcctttcc acctcgctgt cgcgtgcgct
    26281 gctgcggctt cagctttccg gagtaatcaa catgaaggct gagtctgacg ccagcggcgc
    26341 aatgcagtta accggccgga acggcagcgt gctgcgtacc gtaacccatc tgaccttgaa
    26401 tgatgtggga gtccactaat gccacaacta cagagctact ggcccagcga gcagaacgtt
    26461 actgcctgca tcctaaccga ggctgagtca ctggctgaca gccagttgct ggcggtgcat
    26521 gaaccgatgc gtctcgaccg cattgagttt caaagtggta aggtcacgca ggtgggagag
    26581 ggggcgctgc tggactttct gcttgaacat aaccgcccgc tgccgctaat tggcgcttct
    26641 ggcgtgggga aatcccactt ggtacgctgg gttcatgctc agctcaaacg tcgtgaagat
    26701 catgcgaaat accacattat ccgcatcccc aaaaatgctt ctctgccacg tgtacttgcc
    26761 gccattctgg aaggtcttga cggcgaggag tatcagagta ttcgtgaaaa ggttaacgga
    26821 gttggaaagc aattaatccc cgaaaacgtt gccgaacata ttgctcttaa attgcgtcaa
    26881 gcgctaaacg cagcatatac agatgccatt gaagagcttc gccaagaaaa aatgggaaaa
    26941 attcagctgg aagaaagcag aaaaaagcag ctggaagaaa ttcaacaaca cgcagcatca
    27001 tcccgactgc ccgcgctgtt ttttgacagc gtgatgacgc agtatttcac tgctcctggc
    27061 gcctgtcttc acaatatagc gcagcgcttc tgccagggag ctaacgacga cagtattgcc
    27121 aacttgcggt atgaaatgtc tgcggaagac ttcaccttca aaggactgga cttcaggcag
    27181 gtttcaccaa cagtgcttcc ctacctgcgc aaccaacagt tgcttaccag tgatgaaaaa
    27241 aagcaagcag cggctcgcgt tgtcaacgtg gtgcgcagcc aggcgttaga agacaccttc
    27301 gtcgaactat ttagctttaa ccgggccaat ttccaggatc tgatgcgtat gatccgccga
    27361 cagttgctgg cagaaggtcg cgacctgatc cttcttgtgg aagatcttac cgccacctcg
    27421 gcgattgaag atgtcctgat cgcctgtctg atagaggaag atgagtacga cggcaaaaag
    27481 gttttatgta ctctaagatc gattattgcg gttactgagg gtcacgatag ctttaagcgc
    27541 ctacgtaata cactaggcac ccgcgcccgc tacgaatggg tgatccagca gcatgccagc
    27601 gaaaacgatg tcgcgctgaa aaaacgcgtt attgactttt gcggccgcta tctcaacgct
    27661 gcccgtcacg gcgctgctgc tctggaaact tatcacctcc agcaagacgc gcagtgttat
    27721 caggagattc ctgtatggca ggatcaggag gtgctggaga acgaatctgc tgcgccagcg
    27781 ctggccagtt ttggcagcag tagcattggt catccgctgt ttcccttcaa cccagcagcc
    27841 attgggcagt tggtagaacg acactgcaaa gttaaagaac aaggcctagt gtttatcccg
    27901 cgtaatattt tgcatgaaat tttgcgcgag ccactgaaaa agtatcgcca gaattatctg
    27961 gacgggcagt tcccgccatc acaatttgct gagattgtat gtaacccggc attacagcag
    28021 agggtacgcc tggaaggcct cagccagcct gagcggatta attcactgct ggctgtttgg
    28081 ggggggaata gtacaaacct ggcaggtctt acgcccgcca tctgtaaaga gtttggtctg
    28141 ccggatgcgg cagtactgtt ggggggcggt ggaacgccac cggaaccagc accggaacca
    28201 gtaccggaac ctgcaccagt accggaatca gtaccggcac caccatcacc acttgatgaa
    28261 tggaaactcg tgctggataa atggcaccag agcaagacgc tcgagcaaac cagagcccgc
    28321 gacattcgta agtggctcct ggaagcgctg aatgcccgta tcgactggtc aactgagctt
    28381 atccagacct ctctcatggc cagctcagca aaaactcagg gtaggatcca cttgcccggc
    28441 gtgcttgtag ggaatcagtc acatccgctg gtgaagcttg aagatactcc tgagttgcgt
    28501 agcgcctgtc tggcgatggc gcacttcaac cattatcaga gttgggatta tgacggcagt
    28561 gagatcgact acgccgcctt tcatagtttc atggattgca ttgagcacga agtaaaactc
    28621 aaggtgttgc aggaagaacg cgaggagctt tctagtatca tcaacgatct gcagttaagt
    28681 ggtgcattcc ttgggttgga tggattatca gcatcccacc cggccacgct ggtgcaggca
    28741 ctgttaacgg aaccgcaagt cgcaaacagc aaccatccac tgctggatga gttcttaacc
    28801 caagcgcgcg acaccgtgat tgatgagcat ctgaaacgtc gcgagcgttt aatcgcgctt
    28861 acggcggcga aaaaaccggg tgctcagccg tatgctatta acggcttaat gctggtggag
    28921 gcgtctaaga aacaatcgcc agacgctggc aaacaaccgg gcaagatcca tgaagtcaaa
    28981 agtaagctcg gtggctaccg caccctgcta ttgaagaccc aagagagcat cactgaacgc
    29041 ctgagcgacg gcaacagcgc cagcctggca gcggtgaacc tctgcgacac catgaaggag
    29101 atagtcgaac tggcgaatag cgcggacgtc gcccgtccaa tgggtgaaac tgtaccggta
    29161 ctgctgaagt tgctgaagag ctggaaagat ccagagctga aaaaactgat cagacaactg
    29221 gaaaaactga cgttgccagc cgataactcg cacatgttga tgagagaaat ccttgccatc
    29281 aatggcgagc agtttgaggc gtttcgacag ttattagaga attggggcaa gttcgaagat
    29341 gccaccgaca agtatgtgca acagcgtata agcgataagg gcggagacca agttgaggat
    29401 gcagaaaacc aagttacaga taccctggca aacctggctg aacagttaac actgctggag
    29461 gcggacttat catgagtgaa gtaaacatca atgacgtggt aactcccgta gccagcgtac
    29521 cggaagaacg cggtattttt gcacggcgca gcggtgattt taaagcacgc cttcaacgct
    29581 accaacaaat ggagaaagct caggatatcc gcgataacct gctgcggata gcaaaaaaaa
    29641 tcgatactcc gacccaaaca ctagcacagg ccctctgtag ctatagggtg ttaaggcagg
    29701 tccacgacga tgaaacgggt aagccgattg ttggtccgtt tgaccgtgcg actttcggca
    29761 atccacaaat cctgctgacg gccttttgcc agcagtacgc gacgcataaa catgatgttg
    29821 agcaagaggg tgaacttgcc ctgctaactc aagaaattag gcgcttagcc agcgaactca
    29881 gcacgcacac caccgcggcg tgggttaact atattcgcgg attaaaagct cagtgggaag
    29941 tggatcagag actgttcagt aacttggcgc atcaggaagg acaacgaaaa gtacaacagc
    30001 actacattga tctcgtggaa aagttcacca gcagtagccg ggggctgccg aaaaccattg
    30061 aagaatttga gacggtgaga gacctgcatg agaaactctg ccagcaacgc ggagcactta
    30121 agctggatgt gccagaagag gtcaatcact tcctcaaagc cgtagccggg caaggtgcca
    30181 cgctcgatat gttgacacct aatgtactca gttggctgac tgaagaagac gacccgacgc
    30241 gctatcgtat aagacgcctg taaggagccg tagtttcaat ggaacagatt attcgcgcag
    30301 tacttgatgg gctggagtcg caggaggcgg tactgctcac ttggggtaac acacaaggtt
    30361 ttttcactga gaccgagctc cgtaatacta tttctcaggt cctacagaac cacccgctcg
    30421 acgccaacgg cctaactagt tttggcatag tgaaagttat gatcgatcgc ggattgctgc
    30481 tgcgccacca gcaaaatggt gtttcggtgt ttcgtacccg catggcggag tccgtgcggc
    30541 tttacagcca gttgcgccag atgtttccta agcacggctt taatgataac tggcgtactg
    30601 caccgacgtt ggtgtccgac tatcgcttct tacgccgcca gcgcaaatac ccgaagcgtg
    30661 accggcaggc tgctgaagtt ttgcagcaac ttgatgaaat agcaggtttt acctctacgc
    30721 accgccaact actatcagcc ctgctcagcc actcgagcgg aatgtacaac ctcgcctact
    30781 ttcaacaaga agcctgcaaa aacgtactgc gcggattgca gcagagccag caccgctcga
    30841 ctgctaccat tgtctgcgcc ggaactggga ctggtaaaac tttggcgttc tatctgcccg
    30901 ctctgacctt tatcggcagt gaattggtcg ccaaaagaaa agacgcggtg aaggctctgg
    30961 ctatctaccc gcgcaacgaa ctgctgaaag atcagtttat tgaaacgttg cggcagacgc
    31021 gcaaattgaa cactgaactg gcaaagaaag gtgtgcgcaa agtgcgtatc gccgcattgt
    31081 ttggtatggt gccaactaat aaagaggcga tccatcgcga ctacatgaaa gatgcttggt
    31141 gcaggcatcg caatggcatg gcttgtctgt acattccctg cccgacagag agttgtcgcg
    31201 gtaaaatggt ctggcacacc gccgattatg ataaaaacct tgaacgcctg cattgcgaca
    31261 gttgtagcca gaccatcgag cctgacgaag tgatcctgac gcgtaagcgt atgaaaatag
    31321 aactgcctga cattctattc actagcaccg aaatgctgaa taaacagatg ggcaacccgt
    31381 ttctggcatc gctgtttggc atcggcaaaa acgtggtccc accgtcgatg atgctgcttg
    31441 atgaggtgca tacctattcc ggcgtcagcg gtgcacagaa cggcatgctg atccgccgct
    31501 ggcgacatct gtccggtgcc acaccgcact ttgttggact gtccgcaacg ttggagcagg
    31561 cgacgcgctt tatggcttcg ttaaccggct tggaagagta cctggtggcg gaagttagtc
    31621 cgatcgaaga cgatatggag acagagggcg cggagtatat gctggctcta cgtggcgacc
    31681 cggcgtcccg ctctagcctt ttgtccacca ccattcaggc ctccatgcta accaggcgta
    31741 tgctcgacaa cgccgcagtg ccggtcagca acggtatgta cggcacgcgc agttttgtat
    31801 ttacagatga tattgacgtc attaaccgcc tctatttcca gctactggat gccgagggtc
    31861 gttactcgaa tggcaatatc aatgccaaaa aagaaccgct ggcgatgctg cgcggcgatg
    31921 cgccaaatga agagaaattt acctttggtc agcaatggcc gttggctaaa atgattggcc
    31981 atacactcga ttctgctgac cgcagcaacg ttaagcgcac ctcttcgcag gatgctggcg
    32041 tcgaccatgc tgcggatctg attgtcgcta ccgcgtcact tgaggtcgga tttaacgacc
    32101 cgaacgtcgg tgccgttatc cagcataaag ccccgcgcga taatgcccaa ttcctgcagc
    32161 gtaaagggcg tgctggacgt caacgaacca tgcgcccgtg gaccgtggtt gtgctgtccg
    32221 actatggccg tgatcgcatg gcatttcagt gctacgaaaa cctgtttgag ccggtactaa
    32281 aggcgcgcca actgccggtc ggcaacagtt acgtactgcg tatgcaggcg gcctttgcca
    32341 ctatggactg gctctccagc cgtaacgagt atcttaatca gtggaatcgt ggtatctggg
    32401 atgacctatc cgtcccgcag gacaaaggta agccatctga agctcagtca aagctggccg
    32461 atctgattga agaccttctg aacagccaga aaatccagca ggagtttaac tcatggttgg
    32521 ctgaagcact ggggattaag gacgaaaaac tgctgcagtc attgctgtgg caacccccac
    32581 gcgcgattat gaccgcgttt cttcctacgg tacttagaag actgcgttcc aactggtcgc
    32641 gccttggcat tgagcaggcc gataactgcc gcaaaagcac gccgatgcct gattttatac
    32701 cgtccgcgtt gtttaacgac ctctgcctgc cggagttaca gattaacctg ctaggtgaag
    32761 tcggtcagga acccgatgcg cacagcatgc cgattttgca ggggatgaaa gattttgccc
    32821 cggggcgtat cagcaagcgc tttgccatca aaaacattcg cgaatgccac tggctagtgc
    32881 cgaaaaaact cgagctgaaa gatggttcgc atccattccc gattgatgat tactgcccgc
    32941 cagacaaacg ggagtccatg cccgattgtc acattaccac tcgcactggc atgcaggtta
    33001 tcccttgctt ccgcgcctgg gaagtgactg ccagcacgcc accagatgac cttaaactga
    33061 gtgaaacctc aaacgcgttt cttaactggc acagcgagat ccgtccacct cagaacggca
    33121 tccctgcgga agtgccatcc aacaatgcct ggcaggatat tttccagcag gtggagtttt
    33181 acagccacca gcagcactgt ccgatcgaag ccgtgcgctt tgccaccgga tcacgtgcca
    33241 atattaaatt cagcgaccag cgtgaagacc tgcagattga cttcaaattt gaacaccaag
    33301 gcgaacccgc tgcgtttggc ttctcgctgt gggtagatgc gataaaattc cagtgtcggt
    33361 tacctaattt tgatctcgct agcatcagca acaaccgtga actggtggcc ggattgcgca
    33421 cagcgcgctt cctgtatgaa gtcagccacg acgaggcaat cagtgttaat actttcctcg
    33481 gcggttggct ggcagaaact gtcctggcag gtattggcag cgaagcagta ttgaaaaact
    33541 gcaccccgga gcaggcgttc cgcgcaatgg aacacaacag cgcaaggatc gccttgattg
    33601 acgttcctgc tcgaatcttc cagacgttgt atgatgcgca gaccggtgaa gagatagagc
    33661 aggatctaca gcaaagcttg cgtgaactgt tgtcccaggc tccggtgatg gcaagaattg
    33721 ccgactgggt ggaactgctg tggcaaccgc tggataacac ctggttggag tggttgaccc
    33781 tcaacttcac caacacactg ggtgcggcgc tgctgcaaac cgctatgcag ttgtgcccag
    33841 atgccgacga caacgaccta atccttgacc tcaacggtgg gccggtgcgc acttcgctgc
    33901 tcgcgcctga ccagcgcgaa atctggctgt cggaaaccac cgtgggcggt ggcggtatca
    33961 ttgagaagat ccaacagatt taccgtgaag atccgcgcag tttcttcgag ttgctagact
    34021 ttaacctcag tccaggcgac tacgaaacaa tggacaataa tgtctggcac ctgctgcaaa
    34081 ccatggtaaa cccgacatca tccctgcctg cgtgtatgaa cgaaatgcgc cttgccaacg
    34141 atcatgccag ccaggttcag gcgcagcgca acttgctggg agaattgcag cgcagcggct
    34201 ttatgaccag ccacagcttc ctgtcggcga tcaacacccg cctattacgg ccggggaccg
    34261 atgccagcag cgatgtcttt ttactgcaac tgcaacagga ttggcgacag caggagcagc
    34321 gactgggtat tgaactacca cagcgtgtct atgcctttac ctgtagtcag tccagcgcgc
    34381 ttgaccagca actacaacaa gctagccaag ggcagcacaa cttggagagt tggcgtttaa
    34441 atacctgtaa tggcctactg tggccacgtg gccataccat tcgtgatagc tggctaactt
    34501 ggtacaaccc ttacagcttt gccgggccaa ccgagcgcct cctactggcc catgtggtgc
    34561 agcacagttg tgctcaggtg gcgctcagcc aaccagactg gatgcagcag tgtcaccagc
    34621 atctggaaaa taatggtaaa tgcgaattac tagccgaacc gggcgatgca ataaatgagg
    34681 ccattgctac tcttttagtc accccagtag aaatgtatgg gttgttcttc tacccgcgcg
    34741 tcagtgccct gcgccgccac aacaatgaca ccttcgccat gattgaaatt ccggaggcga
    34801 tccaatgaat acccgtcgta tttttaaatc cagaacctca cgccagagtg aagttcttga
    34861 tgtactcagc tccttgttgg tagcggagat cgctcagccc agcgcggaat tgtggatagt
    34921 ctcgccgtgg atcaccgacc tcgacctgct ggataatcgc accggccgtt tcgactacct
    34981 cgaaccagcc tggggacagc gtcaggtgca gacctcagaa ctgctggcgc gtgcggtagc
    35041 caacggcggt acgcttaaag tgatgaccaa cgagacccag cataatgcac gctttatccg
    35101 ccagttaacc gaacgcgtcg ccaactacgg catgctggat aacctgtaca ttggccagaa
    35161 agaggtactg cataccaaag ggttcctcgg agatcatttc ttcctaagcg gttcaatgaa
    35221 tctgacgatc ggcgggatcg tgatcaatga tgaacagatt aacttgacca cagacaaaac
    35281 cgccatcgtg caggcgttgt tagcatttaa cgacttttac caaccggcac agggaggtgt
    35341 gcaatgaaca gcgctgatag cgaaaagttc ctgcgagcct ttttccatag agaaaacaat
    35401 attatctctt gggaaaaaat cgcgggtggc ctgctagaag catcaaccca acagcggttg
    35461 accccctggc ttgacgatgt ccgcaacgga tgtttcccgg tgatcctgcc gcgcgtagta
    35521 aagggcagca atgacaaagt cacctggtat gcaatggccg atgaggaacg agactatgcg
    35581 gagttgcgcg aattattaga cgcgcacgtc ggtacctcgt ggtcagactt taaccgccag
    35641 cgcacgatgc tggatccgag cgacccggtg gaaaaagcgt tgatagacca ctatagcgat
    35701 agagtatata aatttagctc tctttctctg tcgaggatcc cggagatttt caatggcata
    35761 gagcgcttac gccaacaact cgcaagccgc ccgagttacc agctcacact tctgcgcccg
    35821 cgtggccgta ttctgcgtga tattcgtgcc gccttgcgcc gtcgtgatgt gccgcagact
    35881 cgcctctatc tgcaggaact acgtgaagct ggtggcatag gccatattaa cgaactgttt
    35941 atcgaactgc gctgtatggc tctggaaggt gacgccaaaa gcctgctgga ttcgactcag
    36001 ttgcgacagg tcttggatat gccgcacccg ttggtgttgg ctgaagaagt aatgacagtc
    36061 ttctatcagc agtttctgct gaagtatgag cagttgagcg acctcgccgc ccaacgacaa
    36121 gcatttaata atttgctggt gaagaacgta ccgcgtctgt tccaggtgac gcagggtata
    36181 aacacgccat cggcaatcaa aaatattcta ctgtggttac tttctgacac acaaagtgat
    36241 ccggcaagac tgagggaaca ggcacaacaa gtggttgccg gtatgaaggg ggcaacagac
    36301 gatgaccggc actggatccg ccggttgttg gaagcattcc cgaccgttgc agtggcctcc
    36361 ccaccgacgg cgagcatcga tgaattgctt gccagagccg atgaatatcc tgaaagcgtt
    36421 ctgaaagagt tactattgct accggtcagc gctcgcggcg tagccattgc tttgcaatgt
    36481 gcctgggtcg taggcagtgc aggagctgct gtgagtgctg tcagttatct gcaacaactg
    36541 gcacaacaag agcgcgactt gttactggcg aaaccggcct tccagcgtta ttacactgcc
    36601 ttacagttga tgctgccatc caaacgaaca gaacatgata ctgcaggtat caataattgg
    36661 caggactggt tgaaggaagt gttaaataac cctcaatggt cgggggcgat agaagcggta
    36721 gaacaggcct gcggccaatg gaacgcagcg gctttccagc ccgaggcatt tgtttcgttg
    36781 ctggataaaa ccaacaactg cgccagtatg gtgttgcgcg atgccattcc agtactgctc
    36841 gagtggctgg ataaccatca aacaagtgct tcctggcagt ctgtccggtt agcgttgctg
    36901 caacaactgg cgctggacga caatagttct cgcgaagata tctttactgc ctgtgaacta
    36961 gcacgcggtt atttagaggg cgatagtaat gcgcacgatt accgcgaact aatagaaaac
    37021 ctgcagatga tgatcgagag ttcaggtacc accagcgcta gcgcctggct ggatttgctg
    37081 gaactgttct tggtctaccc aactgctgac caacttgcca gagaacaatt attcaatctg
    37141 gtactcaaca tgttttaccg caacccagcc cgctatagtg aattgcagtg gagcctattg
    37201 gctcagtttg ctggcgagtg cggacttgtc tgtgaaattc ccccacaaat tttagaggac
    37261 agtggggaaa aaaatgatga ggttcgtgtc ggatcaacat tgaatgacaa ggtagtgggc
    37321 atttatacat tgactgagtc ggtagcacag cgtgtgaaaa aacaactact agccgattat
    37381 cctcaggccg atattcgcct caacaacgat aaaggcggct caaccatgct gcgttcattg
    37441 gctagagaag cggatgtatt tatttttgcc tggcttagtt ctaaacattc tgcttattac
    37501 gcgataaagg aagagcgagg agataaacct ttattgcaac cttccggaaa aggcagtacc
    37561 agtatgctgg actgcatacg tagttatctt aaattgtagt aaaggtaaaa aacttagctg
    37621 taacctataa cgtcacagct aagttcaatc tcgggctatt caccccgata aacacaaccc
    37681 gcactacagg tttctttcac catgacagca ctcagaattg gcaactgtgt tttaacctga
    37741 ttccatatcc atttggccag tacttcgctg gttgggtttt ccaagccagg gatgtcgttg
    37801 agatagtagt ggtctaggcg atctagcgtc ggcttgaaaa tggccttgag ttcagcgaaa
    37861 tcaataatcc aacctgtgtg tttatcaact tcaccagtta tctcaaggcg cacgacaaaa
    37921 gagtgtccgt gtaagcggcc gcatttatgg ccttcaggaa cattgggaag acggtgtgcg
    37981 gcttcaaatg tgaaatcttt gtataacgta gttttcatgg tgacctcatt ttttgttata
    38041 cagtaacaat aagaagcaag atttcctagc atttaagaat gagcattaac acgcttgcgt
    38101 attccgaccc aaaccggatt gttttcgatt gtgagcctct ttgcaggatg agcatgcaac
    38161 tcaattggac gttatcgcta gccacagccc tacatcgact agtttcaccg cttcctacgt
    38221 aagtttaaag attggcggca aaaaagagct tcagcattct catgacgcta tttcagttgg
    38281 ataactgaaa tctccgctac gacaactcag gcaataacta actggtcttg cattgtgcgc
    38341 agagccagtc ggctttactg accctgagga tagcattggg gatgaagtgc tgtagccgtc
    38401 tggctaatca ggatttacat acgggcagtg ctgtcgcccg ttctccgtta gatcatcggg
    38461 ccggtgttca aaccacgggc gttcggtaat gtattcagga tggtattgca gcacgctact
    38521 gctgtggcag gtataacgat cgtcaggggt ggtcttcgca gtacgggcac cggcgctccc
    38581 gtcgaaagcg atataggatt tggcgtacat agtattgttc cttaccgcca gacgacacgc
    38641 aggggcagac gtcccggtgc tagcagcgtg gttggatgtg aatggcaggt caatttagta
    38701 atatcagaag agggctaagc ctgtgctgga tgtcattact gtctgttagt tttcacacta
    38761 tgaggtactg acgtaaaaag tcagagtgac tggctgctac ggggtctccc tgttaatggg
    38821 cgtgcaggga cctcgttgca gccgtagagc agcaggccat gcttttgctg aattttggtc
    38881 atatacgcca gtacctgtcg gatgttggtg atactgtccg gatcgctgta acgtaccgga
    38941 atgttgatat tggcctcgta atgctcttta aactcacatc ggtgatacca gccttcccgg
    39001 tcagtgatat cgctccagta caaaccggct tgctgtgccc aggtataggt attctgagtt
    39061 ttactaccat ccagccagaa cacgacgtgg gcatgatagt gatggtcttc tgtccattcg
    39121 ataacccaga agaaaccgac caccgcttcg agttgcatca tacggttcat cagctttcgg
    39181 atatcccatt cgagctgctg gtgatcctgc aggaaaaact tagcggtatg atcctggtaa
    39241 aacagatctg ttcggaatgc ccgcaggcag gaatagcgct ctaccatttt gtcgatatgg
    39301 ttgttcagga gtgactgtag aaaccagtct ggctcccatc gttgattgtt ttgcatagga
    39361 tgagtttctt tagtgctggc aagggccagc cagtggaatg aaccaggcct taaggaagga
    39421 ccatggcaga gggagttgta tggattaggg gggatagaag taaaaaggga gtgcctatta
    39481 attatattaa ttaacctaaa ttaattttca ctccccaata accctcaaat cccttgcagg
    39541 actatggatg aagcctaact cagtggttca gctgtcgtcc gccatgcaaa gcagacatac
    39601 tcgtccatca taatggtcag cgcagggttt tcagcctgcc ggttattgat ccagatatcc
    39661 atctctttgt cttttttgaa cctgaggttg tcggtccagt catcaaggca attccccagc
    39721 atcaaatcgt accagcacag ccagaccagc ttcagacgca gctcactacc gctgaactgg
    39781 cggaacaaac tgatgagctt atttgccaga gggcgttgtt ttaaacgcac gcactcagac
    39841 agcaaaatcc cctccagcat gggatggtga gtcagcagat gctcctgggt aaataaactg
    39901 acgctcaggt cttcattaac agtcagtggg gctgctgctc taccataaat aatccggtgc
    39961 aggcactggg ggacaatagc aataacctct tcctctcccg gaaacttaag gctgccaacc
    40021 gtcagggtat gatggtggta gatattctgg tgacacatcc ccgatctgac cgtcagcttc
    40081 gtaaaatcag tcaaccagac ctgataatat ctataaagtt cttcgtaacg aggcttgtaa
    40141 tctttcatag ttcatattcc ttaaggctta aatgacagac agcagaaaac ctccggagta
    40201 accggatagt tattattctg ctgagggttt agtctggtgg gaggttattt acgagactgg
    40261 tcgattcttt cctgtagcca gttttcgact tcgcttttta accatcggga gcttcggccc
    40321 agcttgatgg gtttgggaaa gactccatcc ttaatgagct tataaatcca cttatctgtc
    40381 atttgcagca gactggtgat aaatgccatg tcaacgaact ggtcttcggt cagtgagggg
    40441 cgggtagtca tgatttgtat ctccggttaa gaaaggtaag ccggattagt gaagtttgat
    40501 aacgacgact gctccgggac atatccaggt ggagatacgt aaaaaaagag agttatgatt
    40561 acctctgttc ctactttgat atccgtgcag gtctttttca aataaagaag ataatgaagt
    40621 gtgatttctg gtagggccat tttttagatg ggaaattgta tggcaactag ttgctgtcta
    40681 tattggaagc ggttgccact attaatcaaa aaatgtaaga agagtgtgta ttaactagaa
    40741 tggtaataat ttctatatag cgctgaagag agaaaagtga tcatcattct catttaccta
    40801 tgtaaactac atcacataat tgtcattttt cccttggttc ttatacatga atgttattga
    40861 tatacagtat ggagttatgc taaaagtcat taattcactg taaatcactt agtgatatca
    40921 aggaaggtgc cggtatggtt aagctagtgg ggaatgacag tagtaagatt aaatatcttg
    40981 aaaataaatt aattgagaat ggttatcact tctattctgg tggggttgat aaagattacc
    41041 gggaatatca gttaagggtt ttcaattacc ttgtttcaca gaatgtttct gagcagaaca
    41101 ttaattcttt ctttgctgag gtggataata gctatacaag aggttttcca tccgagagtg
    41161 aacttgattg gtatcgtaat gatccaagag caagcttatg gttgtcttgt gagttatatg
    41221 agaaattaaa ggaagaaact cctaagtaca atattgattt tctatctcca gaagcattgc
    41281 aacctgatca taatgtgaga attgaagcga tacgtcattg tatggatgaa tggcctatgt
    41341 attttacaac accggctgaa ttcattaagg ataaaagtat tgagtgggct gagttacttg
    41401 atcaacatga tttattcaga agtgttagat ccagtaaggt cgatgtctgt tcttggctaa
    41461 gagattactt aagggggaat acatcaatag gtctgaaacg tatttgtggt aattcttccg
    41521 aggaaataat gtcgtggtgc tatgcaagtt actttatttg gagaaaaaac aacctgcatt
    41581 ctccagattc tgtcgagttg ttcattagaa agtttaagag cgcctggtct actcaaaaaa
    41641 ataggaataa aaataaagaa gagaaaaaac ttgtgacaat gagtgtaaat atctctcagc
    41701 aagctcatga tatgctgaga gatatgtcta tgaaagactc gatgtcaaat aacgcaatta
    41761 tagagagtgc tatccttagg ttatataata ttaagaatag taaagtacgt tccaaataat
    41821 ttcagacaga tattatctac gtgaaccccc gctttcctga ctggatcgcg gggtttttta
    41881 atttttcacg ctgagactac tgtatgaata aacctgacgg ccttcaggcc tttgaacaac
    41941 cgcttgcgtc gcttccgtgc acacttcgcc agcttattct tgaacgtatc cagaacctga
    42001 cccattacga gccggtgatt ggaatcatgg gcaaaagtgg tacaggtaaa tcctctctct
    42061 gcaacgaact tttccagggc gaggtgtccc cagtgagtga cgtgaacgcc tgtactcgcg
    42121 acgtactgcg cttccggttg cgcagcggtc gtcacagtct ggtgattgtc gatttaccgg
    42181 gtgtaggtga aaacgggcga cgggatcatg agtaccgggc cctgtaccgt cgaatgctgc
    42241 cggagctgga cctggtgctg tgggtcatta aggccgatga tcgggcactg accttggacg
    42301 agcagttctg gctcggtgtg atgcaacctt acaggcaaaa ggtactgttt gtgattaacc
    42361 aggccgacaa aatcgaaccc tgctatgaat gggataccct caccagcaaa ccttcaacac
    42421 accaacaggg aaatcttaaa gcgaagcagg ctgcgattat ggcgatgttt aaaccacatc
    42481 atccggtctg tgcggtgtcc gccagcactg gatggggcat tgaggatatg gtggcagcca
    42541 tgatgcgctg tctgcctgac cgcgccacca gcccggtggt gacacaactg cacgggagac
    42601 tctgcacaga gccggtaaag tcgcaggccc gtgacagttt cggtgacgct gtgggccgtg
    42661 ttttcgatac ggcagagtca tcgtcattac tgcctgcgcc actgaaaaag gtgattcgta
    42721 ccgtgcgtga tgcggtggtg tcggtcgcac gagccgtctg ggactggata ttcttctgat
    42781 atcgcaattt tctctgcgaa tgatgaactg gttgttaatg atgtgttgat tgtggacacg
    42841 tcttttccac tgcgttttat catcaattaa atcaggcgaa gcatcattat ctgtcagacg
    42901 agcgcccaca aggctggact gctcataaaa aatagttccg atgttgtcca taccctgtcc
    42961 actccttcct ttaaagtaat cattattaat tcatagagtt aacttctcag gagaaatctt
    43021 tcatgacgct tcaggagcgg cgacatgacc ggcttgccgt caggttgtcg ttgattatca
    43081 gccgcctggt gtccggtgaa actctgttta ttcgcaaact ggcaactgaa ttcggggtgt
    43141 cagagcgcac gctgcgtcgt gactttcggg aacgtctgat gtatctcgac ctggattact
    43201 accaggggca gtgcagtctg cgcacggata ttcacagtac gcggcaggaa atagatattc
    43261 tgacctttgc tcaccgcacg gggctggccg gggttttccc gggctttgac cggcgactgg
    43321 tcagcagcct gctggactgt gatgatcgac cctgtctggt atggcatcag gacagacctc
    43381 atcccccggc aggaaagctc tccttctaca ggctggtcag agctatttcg ggatgtcagc
    43441 gcatcaccat tctgactgaa ggggagcgtc atgaggccgt tgcaccttac cggctaatcg
    43501 ctctggacgg ttactggtat cttgtcgctg aacataacag gcaattacgt gtcttccggc
    43561 tgggtgagat tcatctggta aggccatcgc gggaaacatt tattcgcagt gagcttctct
    43621 atcagctgtc ggaggacaaa cattttatct ctgccttacc tcatttccgt tttattcagc
    43681 agtcgctgaa ttcattttct ccatcagaaa gtcgggggca atccggacaa actgattaga
    43741 gatggtctcc acaattgaat gtaaggttaa tctaatgaaa aaattgaaat tattgtcttt
    43801 actgccagtc atgttttttc ccttacttat cgcaggtaat gctcacgcag cacgtcctta
    43861 tagcgggttc ctgcctgcgg cagtgaataa taaaagtggg aatatattct ggtgtgatgc
    43921 ccaccgacag gtcagaaaac tgtgtacagc gacggaagtg gattcaatgg gatggtttat
    43981 tgttgttcgc tcgaaacctt ccagagattg tccgggtgga atatgggggt ggatcactcc
    44041 cagtaaagac gatgtggcaa caggttatac cattacgcca ggtaaagaat tcaacctcac
    44101 catgaaggat atgtgcagtc ccgatatgaa agtaacgttc cgcccgaata aaaaggaccc
    44161 tcgttatgac gatctcgtcg ggatttatgc agggaaagaa gcgtttcgct acaaacaaat
    44221 aaaataatcc aactgttttt tcgttacttt attgttttaa acaacaaaag ctttattaca
    44281 tgatttgagg acaacattat gcgactgcct tatgtaatag ttacattagc tatgctggct
    44341 tctgtatcaa cagcacaggc tattcccaat atgtggacga gtggttttgg gatgggtgtg
    44401 accgaatata tcattaccag cccggataag gtggtgttta atctgaactg tactggcaat
    44461 ccggatgcgc agaatattct gcaacacggt gttgatctca ccctgcctga tggaatatca
    44521 gtaagctctc atgacgacgg gacggaaata acggtcgtca tggataacag tcagtatccg
    44581 ttaccgtcat tcctcggctg gcgaaacggt gacaatgcct gggtgtcatt tattgacgcc
    44641 ctgagccagg ctgcgaattt tgatgtttac gtcaacgata aaaaagtcgg caccttcagt
    44701 ccggggctga agaatacgca aaaagagctg tctgacctca gtgagtgccg gaccacacac
    44761 tacagcgatt agttacttac atttcagacc tctccacccc acaacatacc aacaccagat
    44821 agcaacaggc catgctcctt cgggaacatg gcctttttgt ttttatttca ttatcagata
    44881 aggagttagc tatgcgatta gccagccgct ttggtcgtat aaaccagata cgccgtgacc
    44941 ggcctctgac ccatgaagag ctgatgagtc atgtacccag cgtgttcggg agtgacaagc
    45001 atgaatctcg ttcagaccgt tatacctaca tcccgaccat caccatcctc gaaagcctgc
    45061 agcgtgaagg ctttgagccg ttctttgcct gccagacaaa ggtccgtgac cagagcaagc
    45121 gggaacacac caaacacatg cttcgcctgc atcgtgccgg tcaacttacc gggcatcagg
    45181 taccggaaat cattctgctt aacagccacg acggctcatc aagctaccag atgctgccgg
    45241 ggctgtttcg tggcgtttgt accaacggcc tggtctgcgg tcagtcgttc ggagaggtac
    45301 gggtgccaca taaaggcaat gtcgttgaga aggtgattga aggggcttac gaagtgctcg
    45361 gggtgtttga ccgggtggag gagaagcgcg atgcaatgca gtcactggcg ctgccagccc
    45421 cggcccgtca cgcgctggca aatgctgcgt tggaatatcg cttcggtgag gaccaccagc
    45481 cggtcacggt atcgcagttg ctgaccccac gtcgccggga ggactacagc gatgacctgt
    45541 ggaccgtcta ccagcgcgtg caggagaacc tgatgaaagg tggtctgtca gggcgaaccg
    45601 ctcaggggaa aagcagccgc acccgtgctg ttaccggtat tgatggcgat gtgaagctca
    45661 atcgcgccct gtgggtgatg gcagaaaaca tgctcgagtt tttcgggcgt taaacaacag
    45721 ttcctgcata aggagatagt ttaatgaata cacaaaatct tgagttcgct gagcaggcag
    45781 caataactgc ctcagcggtc cctgacgagt tacgcatcgg cttctggccg cagcactttg
    45841 gctccgttcc gcaatggata accctggagt cctgtatctt tgactggatg gaccgcctgt
    45901 gtaccgatta tcacggcggt atctggagct ttttgacgct cagcaatggc ggcgctttta
    45961 tggctcctga gtcagagcac gatgagaagt gggcattgtt caacagtatg aacggcaacg
    46021 gcgcggaact tactggcgaa gccgcaggga tagtcgcctg tctgatgaca tacagccacc
    46081 acgcttgtcg caccgagtgt gacgcgatga ctgagcacta ttatcgctta cgagacttcg
    46141 cgttgaacca tccagagtgc agcgcaatta tgcatctcat cgactgagtc ataccgaata
    46201 tgaataccac aatatcccca ctgttatcgc agcctgagct ttttccggtg tcggtcatgg
    46261 ctcagcatgg ctgtttactc cctacgactt ccggactgac accctacgcc caacggacca
    46321 ttcgccgcgc tatgaacctg ctggataaat atctgcgtca gcccggtata gcctttacct
    46381 caagcactgc agcccgtgac tggctccggt tacacctggc cagacaggag cgggaggtgt
    46441 ttatggtgct ctacctcgat aatcagcatc gtttactgga gagtgaaacg ctgttcgccg
    46501 gttcggtaaa ccatgtgcag gttcattccc gtgaggtagt gaagtcggcg cttcgtttca
    46561 atgccgcagc tgtcgtgctg gcgcataacc atccctccgg tgatccggag cccagccaat
    46621 gcgatcgcaa cattacgggc aggcttaaag aagcgttaga actggttgat gtgaaaacgc
    46681 tcgatcatct tgttgtcggt cgggatggca ttgtgtcgtt cgctgagcga ggttggttat
    46741 gaaaatcatc agtaaacgtc aggcaatggc tttgtaccga cagcatccag gctctcggct
    46801 gtttcgcttc tgtaccggca aatataaatg gtccggcagt atctgccatt atgctggccg
    46861 ggaagttcag gacatacgcg gcgttctggc tgtatttgct gaacgtcgtc aagaccgcaa
    46921 tggtccctat gtcattcttc gaagcattac tcttaattaa tccttccctt taatcaggaa
    46981 caataatgaa caatcactca gaatccggta caaagccgga gaatcccgcc tgccagcagt
    47041 gggggcttaa atgtgctatt accccctgtt ttggtgcccg cctggtacag gagggcaacc
    47101 gattgcattt tctctctgac cgcgcaggat ttagcggggc atttgccgtc gatgttgcaa
    47161 tgcgccttga ccaggcattt ccgttaatga tgaagcaact tgagctgatg ctgaccagtg
    47221 gagagcttaa ccctcgccac ccacactgcg tcacgcttta ccacaatgga ctcacctgtg
    47281 aagccgatac actaggctcc tgtggttatg tctacattgc tatctatcct gagcaaactg
    47341 agccgcagta aatccccgct tgtcctctta aactcacaga gcaaacatca tgcaaactat
    47401 atcatcacac ccgacacggg cgactcagcc ctgcctgtca cccgttgaaa tctggcagcg
    47461 gttactgact tgtctgttgt cgcagcacta tggtctgacg ttgaatgaca caccgttcag
    47521 taatgaaacc accatactgg aacacattga tgctggagta tcgctgtgcg atgcggtgaa
    47581 ctttcttgtt gaaaaatatg aactggtaag aattgactgt aatgattgct ctgtcatgga
    47641 gaagtcaccg ttcatcacca gtatcgatat tctacgtgcc cggaaagcct ccggtctgat
    47701 gaaacgcaac agtcacaaaa ctgttaccag ggtaacggca ggtcgacttc aggagctata
    47761 agctatggga agtcaattaa tgttgatatc gacaacattg atacaatgct gtccaatgta
    47821 gggaaagcta acagtaacca gatgatgagg agttcgcgaa ggctaaagcc agcttctcat
    47881 ctttatctgt atcatctact cataggggaa ttttgttcac ttggctttgc cctgattcga
    47941 gtcccgaatc caatgaatta atgtgttggt atctgcgttg gtacgcataa aattagtttg
    48001 tttttattta ttttaattca aggagttaat tgtaatgtgc gagtccggcc ttcgcacctt
    48061 cgcaccttcg caccatcgga acatcaatag acgtcaacgg acgttttttt atgactgaaa
    48121 tccgcgccac acaaggcttt tcgctatttt tcgctcaacc taagtcaacc tacatcaact
    48181 tactgatgtg gatacagcta cagctgcggc tatatcccga ttcgataatg tttgtaccca
    48241 cacaaaaccc ttgaaaggct acaaggctga aattctctaa accatagaga tgatttttat
    48301 gatccagtct ttctcttgtt gagatagttc cagtttgctc actcgtgtta agaaggtatt
    48361 gatcacctga acatccgaat tggtgagcct atttaataga gtgggtggta tacctttact
    48421 aactattgct ttgcggtgac tttcgtagtt tttattactc attggtgatc ctcaaatatc
    48481 attgtatggc ggcattgatc gtttccagca acctttactt actcacttca aactgcatcc
    48541 tcgtagactg ttccactaca ataatcgaaa aggcgtggat gtccattcag gtatcccgct
    48601 aatacgtata gcaataacaa tggcttatgc ggcgcgcgct gttcaccctt gtgccagatt
    48661 tttatattgg caattgcctg ctgtagcgat ttactggaag ccattgatat ccctggcagt
    48721 gaaataattg cgatcatgct cgcaaatttt cggctgttca agcctgcaag tgaagatata
    48781 ttactttgat ctcatttgtt tttttgctgc ctgtgtaccc taattgccat gcgatgctgc
    48841 accgacgcta accaccgttt acgccggagg agttaaaagc gttgatggat gcgaataaat
    48901 caaattaatg aacgctcact tttcgcccca aaactgcaaa attgcttcta caaacctgtc
    48961 agaaataatc ccccgacggg ctgccgcgct gaaatgactg cttcgcagtg ccgtcgtcca
    49021 gttctccggt gaccggtacg ctgaaccgta atatcccgtg cttaaatcgt ccgggtcttc
    49081 atcaggcgcg ttcgtcagtc ctctttcacc ttgttgctgg aagtcgacga aaataatatt
    49141 ggctaaaaca ttattttgat aattgccata aataacttca tacgcatgag ggaaattttc
    49201 tttccagtac caggtggtat cgccgcaaaa ccacggcgcg tcagtaatat tattaagctg
    49261 agaatggtat tgttttagat ccctacgaaa ggcttcaatc atatgattaa agtgttgagg
    49321 gtgtgacgcg tagtcactgg tcattaagtc aaattcgcct tgcatccagc atacgccgag
    49381 gaatttgttc tgcggatttt ttgccagtgc ggctcgcgtt ctgctgacta aatcctggta
    49441 cagcggagta tccgttcccc aacgacaagc atcatggctg gctccgtgcc gttctgaata
    49501 tgtcccttcg ctgcccgcgg taaaagccga tccgccacgg caacatggaa cgatgagaac
    49561 ccctgcatta tcaggaataa agggtagtaa tttccgtgca atatgcagtg cctggccaac
    49621 ggtgccgtac tgtgtttgat gattcgttgc cagaggatgg tgataaccct gcatatcctg
    49681 aacatcgtgt gggcagtgag tcagtggaat aatgtcgtta aagtgacatg acgggcctcc
    49741 gggatgcgta tgcgcaaatc tcgctaattg tttaattctg ggatgaggcg catcttccct
    49801 gtccggtaat ggcagtcctt cgccatacgc catggcatta gactgaccag caacggtaag
    49861 aacatagtaa taatcgggcg atattattgc gttcatataa atttcgcgta attgaaatga
    49921 caaattgata gcagggcccc tttaccgggg ccctgaagcg ttaattttgt actgtgactt
    49981 tattatcctt cacagagatc aaaactgaat ccgtcaccgc tttgccgcct gcagtttcac
    50041 cgccaataat caatagacta ttattccagg gcaatgatac tccgtaggcc cgaccttgcg
    50101 ataattcacc cgatttatcc catttcccgt tatgccaaag atgaatatca gtgctatatg
    50161 atttttttag gccttcatgc gcatagttct taccgttctg gtaattttct cgtgaacctt
    50221 tgaatccggc ccctccggca aatataagag aatcattgct tatccccgca aaaccgccag
    50281 cgacgccatc tggtgatgag acgggagcaa gcttattcca ttttaaatta ttaccggtga
    50341 aatcaagttc aaatacggca tccgttcgca atcctggttt ggcttcgcca ttaataagcc
    50401 aggttttatc acctttattc acaagcgccg caccagccgt tccgtaccag ggagattcgc
    50461 cagcgtaact ccattgctgt gttgagggat caaaagacaa cagaaactta ttgaagaaat
    50521 aatcttctgc ttttttgtca aaataatggg cattgatctt gtctatagtt gctgaatctt
    50581 ttccagcctc gttgagatct tcaaaatagc cattgaagat attctggtta acaccgccag
    50641 taacataagc cttgccgttg tgtacaaaag tcacatgacc cgccatgccc atcggtgcat
    50701 gcgacatcaa tttaacccaa ctattggttt tggggttgta tttgtgtacg tcattaaata
    50761 cctgagtcaa gcccttgcta tttttgccaa tgccgccaaa cacatacaga ttgccatcaa
    50821 taaatgccga ggttgcttga tctcttggtc caccagggaa tgcagctaac gctgtccatt
    50881 ttttatcttt ggcctgtgta tccagcttgt accatgccgt acctgcgcta cctaaaccaa
    50941 tgtagacagt gtcgttatca attgctccgg taccactttt aaatggcaca ggagtttcag
    51001 gtaatacaga cgcgtttgcg gcaaatgaag ccatcatgat agcaagcgcc gttattgttt
    51061 tattcattgt gactgtctcc tgtctactac agtttaaatg acacaccaat gcgataactg
    51121 ttttccgata aattatctct gccgttgtaa acaccctgac ggtccaggta gtcatattct
    51181 atgtatggcg taatatcggg cgtcatatgg tattgtagaa caaatgcatt ttccgtcgcc
    51241 catttcttat ggtttgcata gcgataatcg ttctgtttgc tgtatagcgt cgtttgccat
    51301 gcgaaggtga aatcactatt aatatggtaa gtgacatatc catcccaacg atgaacgtta
    51361 tcacgagaca tatcaccgga taagtcttgt tgtcggtaag ctttccagtc gtaacgatag
    51421 cgaatgccaa aattaagatc ttttgtcgcg tcccaggaca gttttacgta gggtccgtag
    51481 cgtgtgccgt tgctgctaaa atgcgttaac attcccgggc gcaccgtcca ttgatcatca
    51541 agtttaatcg cgtaattaac ttcaacctga acatcattga gtgcggcatt ttccttttta
    51601 ttatcatgaa tggtattcca ggtattactt tccatgcttg cccaccatcc attttgccat
    51661 ccctcactga ctttgagtcg agtctcatag gcgtggcttc cactacgata tccaccacgt
    51721 acgtccagtg tcgcagcctg agaaattaat ggggacgaaa agcacagtaa taatacgcca
    51781 gaaagtattt tagccttttt cataaatttc actcatttgt aggatacaga aagcaataca
    51841 aagcccgcat aaacaattag catttat
//
LOCUS       DAZGCS010000020        51046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGCS010000020 DAZGCS010000000
VERSION     DAZGCS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.020"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(296..772)
                     /gene="ybhB"
                     /locus_tag="ACHK01_001274"
     CDS             complement(296..772)
                     /gene="ybhB"
                     /locus_tag="ACHK01_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO8096250.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(831..2120)
                     /gene="bioA"
                     /locus_tag="ACHK01_001275"
     CDS             complement(831..2120)
                     /gene="bioA"
                     /locus_tag="ACHK01_001275"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO8096251.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2207..3247
                     /gene="bioB"
                     /locus_tag="ACHK01_001276"
     CDS             2207..3247
                     /gene="bioB"
                     /locus_tag="ACHK01_001276"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO8096252.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3244..4398
                     /gene="bioF"
                     /locus_tag="ACHK01_001277"
     CDS             3244..4398
                     /gene="bioF"
                     /locus_tag="ACHK01_001277"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO8096253.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            4385..5140
                     /gene="bioC"
                     /locus_tag="ACHK01_001278"
     CDS             4385..5140
                     /gene="bioC"
                     /locus_tag="ACHK01_001278"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO8096254.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            5133..5810
                     /gene="bioD"
                     /locus_tag="ACHK01_001279"
     CDS             5133..5810
                     /gene="bioD"
                     /locus_tag="ACHK01_001279"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8096255.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6389..8410
                     /gene="uvrB"
                     /locus_tag="ACHK01_001280"
     CDS             6389..8410
                     /gene="uvrB"
                     /locus_tag="ACHK01_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO8096256.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8602..9510)
                     /gene="yvcK"
                     /locus_tag="ACHK01_001281"
     CDS             complement(8602..9510)
                     /gene="yvcK"
                     /locus_tag="ACHK01_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO8096257.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      9775..9919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9907..10896
                     /gene="moaA"
                     /locus_tag="ACHK01_001282"
     CDS             9907..10896
                     /gene="moaA"
                     /locus_tag="ACHK01_001282"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO8096258.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            10918..11430
                     /gene="moaB"
                     /locus_tag="ACHK01_001283"
     CDS             10918..11430
                     /gene="moaB"
                     /locus_tag="ACHK01_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO8096259.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11433..11918
                     /gene="moaC"
                     /locus_tag="ACHK01_001284"
     CDS             11433..11918
                     /gene="moaC"
                     /locus_tag="ACHK01_001284"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO8096260.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            11911..12156
                     /gene="moaD"
                     /locus_tag="ACHK01_001285"
     CDS             11911..12156
                     /gene="moaD"
                     /locus_tag="ACHK01_001285"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO8096261.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12158..12610
                     /gene="moaE"
                     /locus_tag="ACHK01_001286"
     CDS             12158..12610
                     /gene="moaE"
                     /locus_tag="ACHK01_001286"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO8096262.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            12747..13451
                     /gene="ybhL"
                     /locus_tag="ACHK01_001287"
     CDS             12747..13451
                     /gene="ybhL"
                     /locus_tag="ACHK01_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8096263.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13596..14300
                     /locus_tag="ACHK01_001288"
     CDS             13596..14300
                     /locus_tag="ACHK01_001288"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO8096264.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            14370..15083
                     /locus_tag="ACHK01_001289"
     CDS             14370..15083
                     /locus_tag="ACHK01_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8096265.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15119..16075)
                     /gene="ybhN"
                     /locus_tag="ACHK01_001290"
     CDS             complement(15119..16075)
                     /gene="ybhN"
                     /locus_tag="ACHK01_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO8096266.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16075..17316)
                     /gene="clsB"
                     /locus_tag="ACHK01_001291"
     CDS             complement(16075..17316)
                     /gene="clsB"
                     /locus_tag="ACHK01_001291"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO8096267.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHYRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17313..18074)
                     /gene="ybhP"
                     /locus_tag="ACHK01_001292"
     CDS             complement(17313..18074)
                     /gene="ybhP"
                     /locus_tag="ACHK01_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8096268.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18207..18617
                     /gene="ybhQ"
                     /locus_tag="ACHK01_001293"
     CDS             18207..18617
                     /gene="ybhQ"
                     /locus_tag="ACHK01_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO8096269.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18579..19685)
                     /gene="ybhR"
                     /locus_tag="ACHK01_001294"
     CDS             complement(18579..19685)
                     /gene="ybhR"
                     /locus_tag="ACHK01_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8096270.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19696..20829)
                     /gene="ybhS"
                     /locus_tag="ACHK01_001295"
     CDS             complement(19696..20829)
                     /gene="ybhS"
                     /locus_tag="ACHK01_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8096271.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20822..22558)
                     /gene="ybhF"
                     /locus_tag="ACHK01_001296"
     CDS             complement(20822..22558)
                     /gene="ybhF"
                     /locus_tag="ACHK01_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8096272.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22551..23546)
                     /gene="hlyD"
                     /locus_tag="ACHK01_001297"
     CDS             complement(22551..23546)
                     /gene="hlyD"
                     /locus_tag="ACHK01_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO8096273.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23549..24220)
                     /gene="cecR"
                     /locus_tag="ACHK01_001298"
     CDS             complement(23549..24220)
                     /gene="cecR"
                     /locus_tag="ACHK01_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO8096274.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24449..25813
                     /gene="rhlE"
                     /locus_tag="ACHK01_001299"
     CDS             24449..25813
                     /gene="rhlE"
                     /locus_tag="ACHK01_001299"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO8096275.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(26045..26527)
                     /gene="ybiA"
                     /locus_tag="ACHK01_001300"
     CDS             complement(26045..26527)
                     /gene="ybiA"
                     /locus_tag="ACHK01_001300"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO8096276.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26647..28797
                     /gene="dinG"
                     /locus_tag="ACHK01_001301"
     CDS             26647..28797
                     /gene="dinG"
                     /locus_tag="ACHK01_001301"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO8096277.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28825..29787
                     /gene="ybiB"
                     /locus_tag="ACHK01_001302"
     CDS             28825..29787
                     /gene="ybiB"
                     /locus_tag="ACHK01_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO8096278.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29928..31013
                     /gene="hcxB"
                     /locus_tag="ACHK01_001303"
     CDS             29928..31013
                     /gene="hcxB"
                     /locus_tag="ACHK01_001303"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO8096279.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31080..31139
                     /gene="ybiE"
                     /locus_tag="ACHK01_001304"
     CDS             31080..31139
                     /gene="ybiE"
                     /locus_tag="ACHK01_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO8096280.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31242..31502)
                     /gene="ybiJ"
                     /locus_tag="ACHK01_001305"
     CDS             complement(31242..31502)
                     /gene="ybiJ"
                     /locus_tag="ACHK01_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO8096281.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31767..32033)
                     /gene="ybiI"
                     /locus_tag="ACHK01_001306"
     CDS             complement(31767..32033)
                     /gene="ybiI"
                     /locus_tag="ACHK01_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO8096282.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32107..32784)
                     /gene="ybiX"
                     /locus_tag="ACHK01_001307"
     CDS             complement(32107..32784)
                     /gene="ybiX"
                     /locus_tag="ACHK01_001307"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO8096283.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32826..35108)
                     /gene="fiu"
                     /locus_tag="ACHK01_001308"
     CDS             complement(32826..35108)
                     /gene="fiu"
                     /locus_tag="ACHK01_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO8096284.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(35372..35632)
                     /gene="mcbA"
                     /locus_tag="ACHK01_001309"
     CDS             complement(35372..35632)
                     /gene="mcbA"
                     /locus_tag="ACHK01_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO8096285.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            35908..36834
                     /gene="rlmF"
                     /locus_tag="ACHK01_001310"
     CDS             35908..36834
                     /gene="rlmF"
                     /locus_tag="ACHK01_001310"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO8096286.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(36831..39056)
                     /gene="ybiO"
                     /locus_tag="ACHK01_001311"
     CDS             complement(36831..39056)
                     /gene="ybiO"
                     /locus_tag="ACHK01_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO8096287.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(39173..39895)
                     /gene="glnQ"
                     /locus_tag="ACHK01_001312"
     CDS             complement(39173..39895)
                     /gene="glnQ"
                     /locus_tag="ACHK01_001312"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO8096288.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(39892..40551)
                     /gene="glnP"
                     /locus_tag="ACHK01_001313"
     CDS             complement(39892..40551)
                     /gene="glnP"
                     /locus_tag="ACHK01_001313"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO8096289.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(40690..41436)
                     /gene="glnH"
                     /locus_tag="ACHK01_001314"
     CDS             complement(40690..41436)
                     /gene="glnH"
                     /locus_tag="ACHK01_001314"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO8096290.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(41840..42343)
                     /gene="dps"
                     /locus_tag="ACHK01_001315"
                     /gene_synonym="pexB"
     CDS             complement(41840..42343)
                     /gene="dps"
                     /locus_tag="ACHK01_001315"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO8096291.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(42642..43529)
                     /gene="rhtA"
                     /locus_tag="ACHK01_001316"
     CDS             complement(42642..43529)
                     /gene="rhtA"
                     /locus_tag="ACHK01_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO8096292.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            43764..43829
                     /locus_tag="ACHK01_001317"
     CDS             43764..43829
                     /locus_tag="ACHK01_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096293.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            43882..44397
                     /gene="ompX"
                     /locus_tag="ACHK01_001318"
     CDS             43882..44397
                     /gene="ompX"
                     /locus_tag="ACHK01_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO8096294.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(44446..46029)
                     /gene="opgE"
                     /locus_tag="ACHK01_001319"
     CDS             complement(44446..46029)
                     /gene="opgE"
                     /locus_tag="ACHK01_001319"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8096295.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(46301..46429)
                     /gene="mntS"
                     /locus_tag="ACHK01_001320"
     CDS             complement(46301..46429)
                     /gene="mntS"
                     /locus_tag="ACHK01_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO8096296.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            46615..47082
                     /gene="mntR"
                     /locus_tag="ACHK01_001321"
     CDS             46615..47082
                     /gene="mntR"
                     /locus_tag="ACHK01_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO8096297.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            47079..48197
                     /gene="ybiR"
                     /locus_tag="ACHK01_001322"
     CDS             47079..48197
                     /gene="ybiR"
                     /locus_tag="ACHK01_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO8096298.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLCAALVGYVLLVILPAN"
     gene            complement(48256..49176)
                     /gene="ldtB"
                     /locus_tag="ACHK01_001323"
     CDS             complement(48256..49176)
                     /gene="ldtB"
                     /locus_tag="ACHK01_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8096299.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            49395..50987
                     /gene="ybiT"
                     /locus_tag="ACHK01_001324"
     CDS             49395..50987
                     /gene="ybiT"
                     /locus_tag="ACHK01_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO8096300.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT        12247 a        12975 c        13620 g        12204 t
ORIGIN      
        1 gacaaaaaat tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtctcaggtc
       61 actaatacta tctaaatagt tgattcatag tgactgggta tgttgtgttt tgtcgtattt
      121 tgtagtctat tatttagact gagtcaatta taagacgttg atattaatgg tttttactgt
      181 ttcacattca acttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt
      241 tgtttttaat acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac
      301 taaacatcgc agtaatcgag gcgcttgcca gagagtggaa atgaacgtta aacccgacca
      361 tcgcgccgct ggcaccttca tcgacatcaa tacgttctac atccagcgcg tgaacggtaa
      421 aaatgtagcg atgagtttcg cctttcggcg gtgctgcgcc atcgtatccg gttttaccaa
      481 aatcggtacg cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt
      541 gcggtaatac gcgtgtatca gcgggtaagt taacaactac ccagtgccac cagccggagc
      601 cggttggcgc atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaactt
      661 catcccacgc cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat
      721 gacgatgcgg caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc
      781 ctttcttgtt tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa
      841 aaaaatgttt catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc
      901 gggagaataa tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccagaca
      961 ccctgttcga caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc
     1021 acaccgattg cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggcgggggca
     1081 agttgctcgc gcagctgtac ttcaatagcc gccacctgct gctgccattc gccggattcg
     1141 ataatcgcca ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc
     1201 ccgtgcataa agcaaccggc ttcgccgtta ctgatggttt ccgcaacctc gcgcgtggtg
     1261 agcgtggcgg aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc
     1321 ggcgcgattt ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg
     1381 atctcgtcgg caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac
     1441 cattccggat ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc
     1501 gctgcgattt catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcacgctca
     1561 tcccattcac catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag
     1621 cctttccaca ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta
     1681 tcgccatgat aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc
     1741 cagtactgca acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga
     1801 aaaacgcact ccagtggttg cggcgtcatc gccaccagtt tgcggcacag ctcaatggct
     1861 ggcgcatgag tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc
     1921 gccgcattaa gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg
     1981 tcaaccaggc gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga
     2041 taaaccggca gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag
     2101 gcaatatcgt ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttacg
     2161 agtctacacc gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc
     2221 acgctggaca ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt
     2281 tgaagcgcag caggtgcatc gacagcattt cgatcctcgt caggtgcagg tcagcacttt
     2341 gctgtcgatt aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg
     2401 ctacaaaacc gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc
     2461 gcgcaaagcg aaagctgcgg gatcgacgcg tttctgtatg ggcgcggcgt ggaagaatcc
     2521 ccacgaacgc gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct
     2581 ggaggcgtgt atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc ttgcgaatgc
     2641 cgggctggat tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat
     2701 caccacccgc acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat
     2761 caaagtctgt tctggcggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt
     2821 attgctgcaa ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt
     2881 gaaggtgaaa ggcacgccgc ttgccgataa cgatgatgtc gatgcctttg attttattcg
     2941 caccattgcg gtcgcgcgga tcatgatgcc aacctcttac gtgcgccttt ctgccggacg
     3001 cgagcagatg aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt
     3061 ctacggttgc aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt
     3121 ccgcaaactg gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca
     3181 gcaacgtctt gaacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc
     3241 attatgagct ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc
     3301 ctgcgtcgcc gttatccagt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc
     3361 cagtatctga acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc
     3421 cgtgcctggc agcagggtgc ggagcaattt ggcgtcggta gcggcggttc cggtcacgtc
     3481 agcggttata gcgttgcgca tcaggcgctg gaagaagaac tggccgagtg gctgggctat
     3541 tcgcgggcac tgctatttat ctctggtttt gccgctaatc aggcagttat tgccgcgatg
     3601 atggcgaaag aggaccgtat tgctgccgac cggcttagcc atgcctcatt gctggaagct
     3661 gccagtttaa gcccgtcgca gcttcgccgt tttgctcata acgatgtcac tcatttggcg
     3721 cgattgcttg cttccccctg tccggggcag caaatggtgg tgacagaagg cgtgttcagc
     3781 atggacggcg atagtgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc
     3841 tggttgatgg tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc
     3901 tgctggctgc aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc
     3961 gtcagcgggg cagcggtgct ttgctccagt acggtggcgg attatctgct gcaattcgcc
     4021 cgccacctta tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg
     4081 ctggcggtca ttcgcagtga agagggggac gctcggcgcg aaaaactggc ggcactcatt
     4141 acgcgttttc gtgccggagt gcagggttcg ccgtttacgc ttgcggattc acgtagcgcc
     4201 atccagccat tgatcgtcgg tgataacagc cgtgcgttac agctggcaga aaaactgcgc
     4261 cagcaaggtt gctgggtgac ggcgattcgc ccgccaaccg tacccgctgg caccgcgcgg
     4321 ctacgcctga ccttaaccgc cgcccatgaa atgcaggata ttgaccgttt gctggagatg
     4381 ctgcatggca acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcgca
     4441 ctatgagcaa cacgcagatc tacagcgcca gagtgctgac gtcttactgg caatgctccc
     4501 acagcgtaaa tacacccacg tactggacgc ggggtgtggg ccaggctgga tgagccgcca
     4561 ctggcgagag cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgttca
     4621 ggcacgccag aaggatgccg cagaccatta tctggcggga gatatcgaat ccctgccgtt
     4681 agcgactgcg acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt
     4741 atccacggca ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac
     4801 cacgctggtg cagggatcgt tacccgaact gcatcaggcg tggcaggcag tggacgagcg
     4861 tccgcatgct aatcgctttt taccaccagg tgcaatcgaa cagtcactga acggcgtgca
     4921 ttatcaacat catattcagc ccatcacgct gtggtttgat gatgcgctca gtgccatgcg
     4981 ttcgctgaaa ggcattggag ccacgcatct tcatgaaggg cgcgacccgc gaatattaac
     5041 gcgttcgcag ttgcagcgat tgcaactggc ctggccacaa aaacaggggc gatatcccct
     5101 gacgtatcat ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa
     5161 cggataccga agtggggaaa actgtcgcca gttgtgcact tttacaagcc gcaaaggcag
     5221 taggttatcg gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag
     5281 gcttacgtaa tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg
     5341 caacggtaaa tccttacacc ttcgcagaac ccacctcgcc gcacatcatc agcgcgcaag
     5401 agggcaggcc gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg
     5461 ctgactgggt gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca
     5521 cttttgcaga ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac
     5581 tcggctgtat taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc
     5641 tggcgggttg ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga
     5701 ccacgctcac ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa
     5761 atccagaaaa tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccatcctgta
     5821 tttggttaaa ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt
     5881 ctaaaataat cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc
     5941 aggcggcggg ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt
     6001 attagagtta gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt
     6061 taaagacggc tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag
     6121 tcactgaaca ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa
     6181 aagccgttgc tttggggata acccggtaag gccggagttt tatctcgcca cagagtaaat
     6241 tttgctcatg attgacagcg gagtttacgc tgtcacagaa atattatggt gatgaactgt
     6301 ttttttatcc agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg
     6361 cccgcccaac tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt
     6421 taaaccttct ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg
     6481 cctggcgcac cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa
     6541 tgtcattgct gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc
     6601 ccagctgtat ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc
     6661 ctactacgac tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa
     6721 agatgcctcg gttaacgaac atattgagca gatgcgttta tccgccacca aagcgatgct
     6781 ggagcgacgt gatgtggttg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc
     6841 tgatttatat ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc
     6901 gattctgcgc cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg
     6961 tactttccgc gttcgtggcg aggtgattga tatcttcccg gcagaatcgg atgacattgc
     7021 acttcgcgtg gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctaac
     7081 cgggcagatt gtttccacca ttccacgttt taccatctac ccgaaaacgc actacgtcac
     7141 accgcgcgag cgcatcgtac aggcgatgga ggagatcaaa gaagagctgg ccgccagacg
     7201 caaagtgcta ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca
     7261 gtttgatctg gagatgatga acgagctggg ctactgttcg gggattgaaa actactcgcg
     7321 cttcctctcc ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctccctgc
     7381 cgatgggctg ctggtggtcg atgaatctca tgtcaccatt cctcaaattg gcggcatgta
     7441 tcgcggtgac cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc
     7501 gctggataac cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta
     7561 tgtttcggcg acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca
     7621 ggtggtgcgt ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca
     7681 ggttgatgat cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtactggt
     7741 caccacactg accaagcgga tggcggaaga tcttaccgaa tatctcgaag aacatggcga
     7801 gcgcgtgcgt tatcttcact cagatatcga caccgtcgaa cgtatggaga ttatccgcga
     7861 cttgcgtctg ggtgagttcg acgtgctggt agggatcaac ttactgcgcg aaggtctgga
     7921 tatgccggaa gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc
     7981 cgaacgttcg ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat
     8041 tctctacggc gataagatca ccccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg
     8101 ccgtgagaaa cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa
     8161 gaaagtggtc gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag
     8221 aggaaaatcg cgcccgattg ttgagccgga taatgtgccg atggatatgt cgccgaaagc
     8281 gttgcagcag aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga
     8341 gttcgaagaa gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc
     8401 cgcatcgtaa caggatagcg aagaagactg atgacaaacg gaaaacagcc tgatgtgcta
     8461 cgcttatcag gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga
     8521 taaggcgttc acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg
     8581 gaattttcct tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg
     8641 taacaactgg cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac
     8701 aatccgctct ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac
     8761 ctttttacca acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc
     8821 aggtaaactc aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg
     8881 taatgcctgg gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc
     8941 aggcccaata atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg
     9001 tacattaggc gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc
     9061 gccgtaaact tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat
     9121 tggaatcaaa tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg
     9181 ccgcacgcta aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag
     9241 ttcgccattg ccaccaaaac ggtattcaaa catcgcggag gcgacgctcg gttccgttat
     9301 cagctggttg aggcagttgc gcatatcgcc ccaggcaatg ccgccttctg aacggcgaat
     9361 acgccccgtc gagccaccat tatcggtggt ggtgacgata cccgttaaac gagaacccaa
     9421 agacgaaagt gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg
     9481 atcaagatca gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct
     9541 acagtagcgc aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta
     9601 ttttcttcta cctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt
     9661 aaagccgcca ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg
     9721 attacatagc gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcataacc
     9781 actaaacact ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg
     9841 ctcgtaaaga ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag
     9901 gtgtacatgg cttcacaact gactgatgca tttgcgcgta agttttacta cttgcgcctg
     9961 tcgattaccg atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg
    10021 agcggcgtca ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc
    10081 ttcgccagtc tgggcaccga aaaagtgcgc ctgacaggcg gtgagccgtc tttacgccgc
    10141 gactttaccg atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc
    10201 accaccaatg gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact
    10261 ggcattaacg tcagtgtcga cagtctggac gcccgccagt ttcacgctat taccgggcag
    10321 gataaattca accaggtcat ggcggggatt gatgctgcat ttgaggccgg ttttgagaag
    10381 gtcaaagtca ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg
    10441 aactggatcc agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag
    10501 ggcagcgagc tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg
    10561 cgtcgcggct ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc
    10621 catccagatt acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc
    10681 acttgcaacc gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa
    10741 ggcggcgtta acctgcgcga tctgctggaa gacgataccc agcaacaggc gctggaagcg
    10801 cgtatttcag cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc
    10861 ggtattacgc aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg
    10921 agtcaggtaa gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt
    10981 cgcggtgaag aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc
    11041 catcacgttg tcgataaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta
    11101 tctgcgtgga tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg
    11161 acggaaggtg atcaggctcc cgaagcattg ctgccgttgt tcgaccgtga agttgaaggt
    11221 tttggtgaag tgttccggat gttgtctttt gaagagattg gcacttccac gttgcaatct
    11281 cgtgcggtag cgggcgtcgc caacaaaacg ctgattttcg ccatgccggg ttcgaccaaa
    11341 gcgtgccgta ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg
    11401 tgtaatttcc atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg
    11461 ctggcgaagc gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg
    11521 cggaagcctt tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc
    11581 acaaaggcga cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcacct
    11641 gggatctgat cccgctctgt catccgctga tgctcagcaa agttgaagtc aatttacagg
    11701 ccgagccgga gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg
    11761 gtgtcgaaat ggaagcatta accgcggcct ccgtggcggc gctgaccatt tatgacatgt
    11821 gcaaagcggt gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg
    11881 gcaagtcggg tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg
    11941 cgcgagttgg tgggaacaga tgcaaccgaa gtggctgcgg atttcccaac tgttgaagcg
    12001 ttacgccagc acatggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa
    12061 ttactggctg ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac
    12121 gaagtagctt tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt
    12181 tggtccgcag ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga
    12241 cggtgcggta gtcaccttta ctggtaaggt gcgcaaccat aacctgggcg acagcgtcaa
    12301 agcattaacc ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga
    12361 tgaagcgcgt aaccgctggc cgctggggcg cgtcactgtg attcaccgca tcggggaatt
    12421 atggccgggc gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt
    12481 tgaagccggg cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga
    12541 agccacgccg gaaggcgacc gctgggttga agcccgggag agcgatcagc aggcggcaaa
    12601 acgctggtag ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc
    12661 agtacatacg tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat
    12721 attccttata tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac
    12781 aaccccgggc tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct
    12841 tgttgctgac cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt
    12901 tgttcactaa ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg
    12961 tgttatcagc gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt
    13021 attcggcgct gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta
    13081 tcgccagtac tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca
    13141 ccacgaagcg cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg
    13201 tgctggcatc gctggtcaac ttctggttga aaagcgaagc attgatgtgg gcagttacct
    13261 acatcggcgt gattgtcttt gtcggattga cggcgtatga cacgcagaaa ctgaaaaata
    13321 tgggtgagca gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggcg
    13381 cgttaacctt gtatctggac ttcatcaacc tgttcctgat gttgttgcgg atcttcggca
    13441 accgccgtta aattctcttc ttcctgctga tcccggcctg gagccgggat ttttttcgga
    13501 ttttataaag actattccgc tcatttacat gagaactgaa tcgcggcaaa tgccagacta
    13561 tgtccactac gtaatttcag ggacagataa cgcatttgaa cagacaatcc actgctactc
    13621 aattacagca aaaaagtgct tcgctcagca aaatgctgct tctcatttgg tggttaacag
    13681 cagctggagt atgggctacg gcaggctgtg cctggatatt tcagcatttg cattgggtta
    13741 cgtcacaaaa gtctccaggt gcgtttgtct tcgctttggc tgcgctgata ataggtggtc
    13801 tctggttaaa caacgccctg aaaagactga atgtatcagg cgcatgtctg gtctatctgc
    13861 taataatcct tcttgttggc tttctcttcg ccagccttta taccttttcg agcatagcga
    13921 cggtaatggg aattacggga gggatgtttg ccgcaatggc gctgatttgc tcttgtagta
    13981 atagggttat cccaccagtc agacagttat attcatatat tttctgtggt ttgagtattg
    14041 cttttgttgt taacctgatc cttaccagca gtttttccgt gtggttagcg agtattttga
    14101 ctgtctttat ctggggcatt acggctgcct gtgaagcaac aacactggaa gatttgatcc
    14161 gtgttgccga cacctctgaa atttcaggtt ctcttcgctg tatcgttcct ggggcaataa
    14221 cactctattt tagtatttta agcgtcctct ttagaattac tgtaacagtt cttgaattta
    14281 ttaatggttt agtgtggtag tgcgaattga cgagttttat tgcgttaatt ggaatagata
    14341 atgatattta acttaaggat ataaaaataa tgaattcgcg ctcagcaaat aacataacta
    14401 atgattttca gcacgaagcc aggatattaa acagcatatg gctcattacc gctttaggtt
    14461 tggtgacaac cgcaggactg gcctgggtag cgaagtatct cgaaattacg gcaacaaaat
    14521 atgattcacc gcaaatgtat gtcgccatag gattattatt actttgtatg tatggcttaa
    14581 gtaaggatat caacaagata aatgccgtca tcgcaggcgt aatatatcta tttttactct
    14641 ctttggtggc gattgtcgtt gctagtttag ctcctgtacc tgccattatc gtcgtgttca
    14701 gcactgcggg cgcgatgttt ttaatcagta tgctggccgg tttattattt aatgttgatc
    14761 ctggttctca ccgttttatc attatgatga cgttgacagg gttggccctg gtgatcatca
    14821 tgaatgccgc attaatgagt gaacgacccg tttgggtaat aagttgctta atgattgtgt
    14881 tatggtcagg cattatctcg catggacgaa ataagctcct tgaattggcg gggaaatgcc
    14941 atagtgaaga gttgtggagt ccggttcgtt gcgctgttac aggtgcatta acactctatt
    15001 actattttat cggcttcttt gggatacttg ccgcgatagc tataacgctt gtctggcaaa
    15061 ggcatacgcg tttttttcat tagtcgtaaa tgcagatact aaagaactta actaaacttc
    15121 acatcgccgc ttcatttttc gcccgtagct tcttcgcctg gctttccagt agcaggtaac
    15181 agatcagcgc cagcagcagc gggataaagt aatacaggac gcgataagcg agcagggcgg
    15241 cgataatgct ccctttcgag gtatgctccc cagccagtag tgcgataaac accgcttcca
    15301 gcacaccgat ccccgccgga atatgcacga tgacgccagc aatactacta accagtaaca
    15361 cgcccagtac aaagaaatag ttcacgcttt gaccaagtaa cagccagata atcgccccca
    15421 ttaccatcca gttaacgctg gaaatcagca tctgagcgag ggcgaatttc catgaaggca
    15481 gcaccagctt ttgccctttg atggtcatat ggcggtgctt cgcgaaagcg caaaaccaca
    15541 aataaaccgc gataatcatc agtaagccaa tgccgagaat acgcagcgta gtttgatcga
    15601 cataccagtg atccggcaac tccaccacgc ctgcggtaaa gataatccct gccagtaaaa
    15661 tatagcccag ccagttggtg gtaatactga gcgagaaaat ccgcgtaata gtgctgcccg
    15721 gtaaccctag tcgggagtac aaacgatagc gcataccaat gccgccgacc caggtactga
    15781 gcgtcaggtt gaaggcgtag cagataaacg acaccagcat cacctggcgc ttcgccagtt
    15841 tgtgaccgca gtaaaagcgg gcgagcaggt catagcagcc gtaaatcaga tagctgacga
    15901 ccaccagccc gaccgcacta agcagcgcaa cgcgattgta gtcgcggatg accttccaga
    15961 cctcttccca gtccactttt ttggcgtaga ccaccagcaa cacaatcacc gcgataaaaa
    16021 acagccaggt gaggatcttc tttgctaagc gccagcgcgg gtgtgattta ctcatcaggg
    16081 ttttaccccc gtgttttcag tttctacccg atcctgcgtt tccattgtcg gttgtgcggg
    16141 cggatcaacc tgcgccagac gtggtgtgtg tgccggaagc cagccaacca gcgccgggaa
    16201 gtggcgtaaa aagtggaacg ccagcacgct tttggtcagg ttccaccagg tgcgtttcgg
    16261 cagcatggtc tcatcgacct gctgacaatc tgcggcaata atgccgttca gattatcgcg
    16321 cagcgtctgg ttaaaatgac gatagtggat gatgacattt gcttcgagat tcagtgacaa
    16381 actgagcgga tcgagattac tggaccctac tgtcgcccag tgatcgtcca tcaatgccac
    16441 tttgccgtgg agcgggcggc ggcggtactc aaatacctga acgccgcctt taaccagata
    16501 gttatacagc aagcgcgcac cgactctgac aatcggcata tccggttcgc cctgaatgat
    16561 cagtttgatc cgcaccccgc gccgtgccgc tttacgcaag gcgtgtaaaa atcgatagcc
    16621 ggggaagaag taggcgttgg cgataatgac ttctcgccgc gcctgagtga gcattttcag
    16681 atagtgacgt tcaatatcat cgcgatgttc ttcgttatcg cgccagacca gcaatacctg
    16741 cgcttctccc ggctggcggt tctcttccgc tttgtgatga cgtcgccacc agcgtcgtgc
    16801 cgcgctctgt ccaggcaggt tttccagctc aaactggaga atatcttcga caatcggccc
    16861 ttcaaggcgt accgcgtaat cctgtttagc ctctggaccg tagctggaca tatgctcggc
    16921 ggagtaattc agcccgccaa taaaggcgat acgcgcgtcg atcaccacaa ttttgcgatg
    16981 catccggcga aacacattgg tgcgcatacc aaaaaggcga gggcggggat cgtagtagcg
    17041 aaataccacg ccagctgccg tcagttcatt gacaaactca tcgctgagat ccggcgaacc
    17101 gtagccatcc agcaagactt ccgctttaac cccgcgttgc gctgctgcca gtagtgccgc
    17161 atgcagctgt ttgccgacgt catcctcaaa ccagataaac gtttcaagaa tgatgcgttc
    17221 ttgtgcctcg ccaatcgcct taaacaccgc gggataatat tgctcgccgt tttccagcaa
    17281 ctggatctta ttgccttcgc gccagctaca tttcataaat gaatctccgc acttaaaggg
    17341 gcatgatcag aaaggtgtcg ccatgtccgc agcggcaacg cggttggcgc gctggcgctg
    17401 gcatttttga cgtagatcct gtccagtcgt agtagaggaa attgcaccgg aaacgtgcgc
    17461 gccgggcgtc cgtgggcgcg ggtaaaaatc tcatccagtc cggcctgcac ttttaacgga
    17521 tgattagctt tttgccgcca gtcattgaaa tcacccgcca ccaataccgg ttcgccgtcc
    17581 ggtagctcat tcacccattc ggcgagcatc gcaagctgcg cctgacggtg cgcctcacgc
    17641 aggcccagat gtacgcacat cacatgaatc gcttttccgg tcatcggtgg cacaatgcgg
    17701 cagtagagca cgccgcgctt ttccgcacca tcgaccgaaa catcgcgatt ctcataatgt
    17761 tcaatgggat aacgcgacag tacggcgttg ccgtgatgcc cttccgggta tacggcattg
    17821 cgaccgtagg caaaatcgct ccacatcgtg tcggcgagaa actcgtagtg cgaggtatcg
    17881 ggccagtttt ccacatgcag cggatgaact tcgtgcgcgc ccatcacttc ctgcaggcaa
    17941 acaatatcgg cgctgacggt acgcaccgcg tcgcgaagtt ccggcaaaat gaagcgtcgg
    18001 ttaaacgcgg taaaaccttt gtgaatgttg atggtgagca ccttgaacga aaattgttgt
    18061 gtttgatcgg gcataatttt cctgtctttg cctcttatct cattgaaata gtgtagtcgg
    18121 cgtcacaaaa aggtgcggtc ttacggaatt ttccgtaaag ttcggtactc tgagtaagta
    18181 gagataaatt cttcaggaga gaagccatga agtggcaaca acgtgttcgt gtcgcaactg
    18241 gtctaagttg ctggcagatt atgttgcatt tactggtagt ggcgctgctg gtggtgggct
    18301 ggatgagtaa gactctggtt cacgtcggcg tgggattatg cgcactgtat tgtgtcacgg
    18361 tagtgatgat gttggtgttt cagcgccacc ccgagcaacg ctggcgtgag gtggcagacg
    18421 tgctggaaga gctgaccacg acctggtatt ttggcgcagc gctgattgtg ctgtggctgt
    18481 tgtcccgtgt actggaaaac aactttttgc tggcaattgc agggctggca atccttgccg
    18541 gcccggcggt agtgtctttg ctggcgaaag ataagaagtt acatcacctt acgtctaaac
    18601 atcgcgtacg ccgctgaccc tgtcgtggcc gttatcacca gtagcggcca caaactattc
    18661 cacacaatat ccagactcgc atccttcaaa taaatctgct tggtaatgtc cgtaaagtgg
    18721 cgaatagggt taatccacgt caggttttgc agccatactg gcatgttttc caccggagaa
    18781 acgtaaccgg aaaggagaat ggcgggcatc ataaaaacaa acacgccgat aaacgcctgc
    18841 tgttgtgtcg agcagagtga tgaaatcaac agaccgaatc ccaccagcga taaaccgtaa
    18901 atcaccatcg taaagtagaa cagcgccagc gatccggcga aggggatttg atacgcccag
    18961 ataccaatcg ccagcacaat ggtggcctgg aaggtggcga caattaacgc cggtacggct
    19021 ttgccgatga agatctgcca ggtggtgagc ggcgaaacca gtagctgatc gagcgtacct
    19081 tgttcacgtt cgcgggcgac ggaaagtgaa gtgacgatca ttacgccgat agtggtgatc
    19141 atggcgatca gcgacggcac cacaaaccat ttgtagtcaa gattcgggtt ataccagttg
    19201 cgtaccacca gctcgctgtt gttaggtttc ggttttcctt ccagcagctc ctgctgataa
    19261 tttttgacga tctgttgcag gtagttggcg gcaatttgcg cactgttgga gttacgcccg
    19321 tcgaggatca actgcaacgg cgcggtctgg aaggtatcca gtttgcgcga gaagtcagcc
    19381 gggaaacgca ccagtagtaa cgccttttgt gtgtcgatgg ttgggcggat ctcctgtggg
    19441 cttttcagca gcagcacatg agtaaaggcg ctggcgcggg caaaacgttg ggtcagctcc
    19501 accgaatgct cgccgttatc ttcatcgtag atggcgatgg tggcgttagt cacttccagc
    19561 gtggcggcga acgggaacag aatcacctga ataagcacag gtaaaatcag aatcgcgcgg
    19621 gtttgcggtt cgcgcagcag cgactgcaac tctttgcgga ttaacgtcca taagcgatga
    19681 aacatgctct tctccctaat ccagccgacg tttggttttc agccacgtca ggccgataaa
    19741 catcaccgcc gaagcgatca aaaacagcac gtttaccacc agcaccactg gaatattccc
    19801 ggcgaggaac aggctttgca gggtgctgac gaaataacga gcgggaataa tgtacgtcac
    19861 cgcgcggatc accgcgggca tactgtcgat ctgaaaaata aagccggaaa gcataatcga
    19921 cggcagaaaa gcggcgttca gggcgacctg agcggcattg aactggttgc gggtaatcgt
    19981 ggaaatcagc agccccatcc ccagggtact gagtaaaaac aggctggaga taaaaaacag
    20041 aatcagcagc gacccgcgat acggcacgcc gagaataaac actgacacca gcatacacag
    20101 caacatcgcc agcatcccga gaaagtaata agggatcagc ttacacagca gcagttccgt
    20161 gcgggtaatc tccgtagaga gcagagcctc catggtgccg cgttcccatt ctcgcgccac
    20221 caccagcgag gtgagaatcg cgccgatgac cgtcatgata atggtcaccg caccggggat
    20281 aatgaagtgc tggctaatcg ccgccgggtt aaaccagtag cgggtttgta catcaataag
    20341 cggttcaaaa gtctgcccgt tgtcctccgc tcgctgcatt tgccagatct gccagatccc
    20401 ttcgacatac ccctgtacaa agttagcggt attcggctca ctgccgtcgg tgatcacctg
    20461 aatcggtgcg gtggcgttgg cgcgctccat ctgttccgca aaatccaccg gaataaccac
    20521 cagaccgcga attttccccg cctgcatttt ggcgatcagt tcctgacggt tatcgctgat
    20581 ggtggcgtcg atgtagggcg aaccggtcat ggtgtgggtg aaatccagcg ccgcttcgct
    20641 acgctgttcc agtaaaatcc cgacccgcag cttgctggag tcgaggttaa tgccgtaacc
    20701 aaaaataaac agcagcaaca gcgggatcac taccgcaatc agccagctac tcggatcgcg
    20761 aacgatctgc cgcgtctctt tgacgcacag cgcccgcacg cgacgccagg aaaggatcgg
    20821 gttactcatt gctatgctcc ttatcccagt cgtggatcaa ctgaataaag gcttgctcca
    20881 tggtgggatc gggttgctca tcgttagccg actgtgcttt caaatcgtcc ggcgtgccgc
    20941 tggcgattaa tttcccgcgg tacaccaggc cgatgcggtc gcaatattcc gcttcatcca
    21001 taaagtgggt ggtgaccatc accgtgacgc ctttctctac catgctgttg atgtgcagcc
    21061 aaaattcacg gcgggtgagg gggtcaacgc cggaagtcgg ttcgtcgaga aacagaatat
    21121 ccggttcatg catcagcgaa caggccagcg ccagccgctg tttaaaacct aatggcagtt
    21181 catcggtggc gtgggaggca atacttttca ggccaaatgc ctcgctcata cgggagattt
    21241 tttcgttctg cgcccgaccg cgtaagccat acacaccaga gaaaaagcgt aaattctgtt
    21301 cgaccgtcag gttgccatag agcgaaaact tttgcgccat atagccgaga tgctggcgcg
    21361 ctttaccgga actctctttc agatccatcc ccagcaccag cgcctgaccg gaggtcggca
    21421 ccagcaagcc gcacatcatc ttaaaggtgg tcgatttccc cgcgccgttt ggcccgagca
    21481 gaccaaaaat ctcccctcgt ttaacggcaa agttgacgtg atcggtggcg gcaaaatcac
    21541 caaatttctt ggttagttct ttcgcttcga tcaccgtctc gccgggtgtg ccttctaccg
    21601 tatgcaatat tgcacccagc ggcgattccg aggttccggc accgcccagc aaatcaataa
    21661 acgcatcttc aaaacgcggc gtagtttcgt tgatgttgat ttccggcatc ccttcggcat
    21721 ggcgaatatc gtctggtgtg gcctcttttt tgaggatcag acgtaccgat ttcccctgaa
    21781 tcatgccgtc gctgacctgt ggcagtttca aggcgcgttg caatagtttg cggttgccct
    21841 cgtgtggact ggtcatcaga aaactgcgtc cggccatggt ttgtgtcagg gcttttggtt
    21901 ccccctgata cagcaactcg ccttcgttca tcagtaacac gtcacggcac tgctcggctt
    21961 cgtcgagata cgaggtactc cagaggatta acatcccttc gcctgccagc tcatgcacca
    22021 tctgccacag ttcgcgccgt gagatagggt caacgccgac gccgggttca tcgagcagca
    22081 acactttcgg ttcgcccacc agggtacagg ccagaccgag tttttgtttc atcccaccgg
    22141 agagcttgcc cgccaggcgt ccggtaaacg gcccaagaga cgtaaactcc agcaggcgag
    22201 caaaagtttg cttacgtgcc tcgccggtga cgctgcgcaa atccgcgtac agattgaggt
    22261 tctccatcac cgtgagatct tcatacagac caaatttctg cggcatatag ccgagcacgg
    22321 cgtgcagcgc gccgtcgttt ttgattggat caaagccaat caccgtggca ttgccgctgt
    22381 cgggtttcag taatcccgcc agcatccgca ttagcgtggt tttacctgca ccatccggcc
    22441 ccaccagccc cgtcacataa ccggcgtgaa tggtacaatc gagcggcgcg acggcgggct
    22501 tgtccatgcc cggaaagcgt ttttctaggc cgttcagcgt gataacggca tcattcatgt
    22561 cctgcctcgt taccgaattg caccgtcacc ggcattccct ggcgtaacgc atcatcggcg
    22621 tcggtcacca caatacgcag gcgatagacg aggtcggtac gcagatccgg tgtttcgacg
    22681 gttttcgggg taaattcagc agtcggcgaa acgaaaccaa tctgtccgtg atagggcttg
    22741 tccgggcgac catcggtata aagcagcact ttgcgccccg gctgggcctg gtcaagattc
    22801 cgttcatcaa cataggcacg tacccacacc ggacgcgtta gtgaaacggt aaacaccgtg
    22861 ccaccttcat tgagaaccgt gcctggctcc accgcgcgcg ttaacagcgt gccatcagac
    22921 ggtgctatca acgttgagtc ctgtaaattc agctccgcct gcgccagttg cgcctgcgcc
    22981 tgttcgaggc tggctttcgc ctgagcgatg tcctgttcac ggttaccgga acggtactga
    23041 cgcaatttat cctgtgctga tttcagcgtt gcctgtgcct ggtcgcgcga ggagcgggca
    23101 ttttccaggt catttgccga aatggtgcgg cttttccaca gtccttgctg gcggttatag
    23161 aagttctgcg catagtcata ggcggcttga gcctgtttca ccgctgcggc ggcctgagcg
    23221 atttcttcat cgcgataccc ggcaagcatc agatcatact gcgcctgtgc caccgagacg
    23281 cccgctttcg cctgcatcag ggcaatctca tacggcttgt gatccagttc gcccagcacc
    23341 tggcccgctt tgatagcatc accttcgtcc accgccagcg attcaacgcg ccccccaaca
    23401 cggaaactaa gatttaccgt acgaatatcc acgttgccat acagcgtcag gccgttatcc
    23461 tggcggcttt gataccacca gtagcctccg gcaaccacgg cggcaagtac cactaccgcc
    23521 aatccgatca cgacaggttt tttcatcact acaaactcct ttgcgataat ccttgcaaaa
    23581 tcaggtcgat atgacaggtc accgtctggt tgatcagttc ggtcttttct tcatcgaacg
    23641 cggtccagcc ggtacgtaac agaattgttt ctttgccaag acggaacgcc agaatctcac
    23701 caatcaacgc atgagtatgg aggatcatgc gggtatcgtt ggcgtcgcag ccggtccagg
    23761 cggcaatcag gcgtgtcagg tggctgtgta gcgggctaat cacctgttcg tgcaccaggt
    23821 gataggctgc cgtgggagag agctgctcac gggagataaa cttgctgagg ttgacggtgt
    23881 catcctgggt gagcagctta atcatgttcc tgcaggcgcg aaggatcagt tcacggatgg
    23941 ctgcgcgatc aggctgtggt tgtgcgaaca agcgttcggc ctcttcggcg tgcggacgga
    24001 actgctcgcc aataaaatcg gcaatccact gggcgcaggc gaggtacaaa tcttctttcg
    24061 aaccgaagta gtaggtgatg gcagcgatat tctgcccggc ctgggcggct atctcgcggg
    24121 tggtggcgtt cattccatat tcaccaaact gcgccagtgc ggcagcaatc agctgttttt
    24181 tcgcctgttc acccttgatt gtcatggcag gattattcat cgcacagtcc attcttaatc
    24241 aaatgattga ttaagattat gactccatat gggagttgtc cagtatggct aagaatttta
    24301 gctacgccag tcacagggat aatttatgcg ctgcgtcaca aaaactgcta cactccgctc
    24361 cctcatgaca ttgtggtttt tgtcattttc cttttcagta tctccctgaa aactacaccg
    24421 gtaacggtcg gggcggttcg gagtagttat gtctttcgat tctttgggtt taagccctga
    24481 tatcctgcgc gccgttgccg agcagggtta ccgtgaaccc acccctattc agcagcaggc
    24541 gatccctgcg gtgctggaag gccgcgacct gatggctagc gcccagaccg gtaccggtaa
    24601 aacagcgggc tttacgctgc cgctgttgca acacctgatc actcgccagc cgcacgccaa
    24661 agggcgtcgt ccggtacgtg cgctcattct taccccgacc cgtgaactgg ctgcgcagat
    24721 tggcgaaaac gtccgtgatt acagcaaata tttgaacatt cgttcgctgg tggtgtttgg
    24781 cggtgtcagc attaacccgc aaatgatgaa gctgcgcggc ggcgttgatg ttctggtggc
    24841 aacaccggga cgtttgctgg acctggaaca tcaaaatgca gtgaagctgg atcaggttga
    24901 aatcctcgtc ctcgatgaag ctgaccgcat gctcgacatg ggctttatcc acgatatccg
    24961 tcgcgtgtta acaaaactgc cagcgaaacg ccagaacctg ctgttctctg cgaccttctc
    25021 tgacgatatt aaagccctgg cggaaaaact gttgcacaac ccgctggaaa tcgaagtggc
    25081 acgccgcaat accgcgtctg atcaggtgac ccagcacgtt cactttgttg ataagaaacg
    25141 caaacgcgaa ttgctgtcgc acatgattgg taaagggaac tggcagcagg tgctggtgtt
    25201 tacccgtacc aaacacggcg ctaaccatct ggctgagcag ctcaataaag atggcatccg
    25261 tagtgcggcg atccacggga ataaatcgca aggtgcgcgt acccgtgcgc tggctgattt
    25321 taaatcgggt gacattcgcg tactggtggc gaccgatatc gctgcgcgcg gtctggatat
    25381 cgaagagttg ccgcacgtcg tcaactatga actgccaaac gtaccggaag attatgtcca
    25441 ccgtatcggg cgtaccggtc gtgcggctgc taccggtgaa gcgttgtcgc tggtgtgtgt
    25501 tgatgaacac aaactgctgc gtgatatcga aaaactgctg aaaaaagaga tcccgcgcat
    25561 tgcgattccg ggctatgagc cggacccgtc aatcaaagcc gaaccgatcc agaacggtcg
    25621 ccagcaacgt ggcggcggcg gtcgtgggca aggtggtggt cgcggtcaac agcaaccacg
    25681 ccgtggggaa ggtggcgcaa aatctgcaag cgcgaaacct gcagaaaaac cgtctcgccg
    25741 cctcggcgat gccaaaccgg caggcgaaca acaacgtcgc cgccgtccgc gtaaacctac
    25801 cgctgcgcag taatctttta tgccgggcta tgcccggcat caggctgatg aacaaacgca
    25861 aaactgcctg atgcgctacg cttatcaggc ctacgtgaac tctgcaatat attgaatttg
    25921 catgcttttg taggccggat aaggcgttta cgccgcatcc ggcatttcac aacaagcact
    25981 tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt taccgcccat aagccaccga
    26041 gacgttactt ctctatagcc aattgttcgc gcaactccat taaaaggtag cccagtcgat
    26101 tcttgccctt accattacca ccgtctcccc agtaagcatc gttttctgta tgctcaacca
    26161 gttttgcggg cgcggttgcc agcaagagcg cacgcagttc tggatgctgt tcgaatttag
    26221 cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc ccagttttta cgcagaggct
    26281 tagaacgatc gcgccccatg cgtgcggcga ccatagggga agaaacccga cggatctctt
    26341 cgcggtattt ttcatcaagg aatttttgtg cctgaaaata gtgttctgag gtgggccagg
    26401 ttttcccgtc aaccttgatg ggccaggcgg caaagttaga aaaatcaccg tagtcatcgc
    26461 tggtgctgta gaaatttatg atggtgtctt gcatgacgtg ttggatcctt tgtgctcgaa
    26521 cgggcattaa accgcattat gttggtggtt attgcgagcc gctttccaga aacagaaaaa
    26581 ccattacccc tgaaaaccga aaaatgccac aatattggct gtttatacag tatttcaggt
    26641 tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg ccgcctggta taaggcgctt
    26701 caggaacaga tccccgactt tattccccgt gcgccgcagc ggcagatgat tgcggacgtc
    26761 gccaaaacgc tggccggaga agaagggcgg catctggcga ttgaagcccc caccggcgtt
    26821 gggaaaacgc tctcctattt gatccccggc atcgccattg cccgcgaaga gcaaaaaacg
    26881 ctggtggtga gtaccgccaa cgtggcgttg caggatcaga tttacagcaa agatttaccg
    26941 ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg cttttgggcg tgggcgctac
    27001 gtttgcccgc gtaatctgac ggcgctcgcc agcactgagc ccacgcaaca ggatctgctg
    27061 gcgtttcttg acgacgaact gacgccaaat aatcaggaag agcaaaaacg ttgtgcgaag
    27121 ctgaagggcg atctcgacac ttataaatgg gatggtctgc gcgatcatac tgatatcgct
    27181 atagatgacg atctctggcg tcgtttaagt accgacaaag ccagctgcct caaccgcaac
    27241 tgttactact atcgtgaatg cccgtttttt gttgctcgtc gggagattca ggaagcggaa
    27301 gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg aaagcgaagc cgtactacct
    27361 gacccgaaaa atctgctgct ggtgctggac gaaggtcatc acctgccaga tgtggcccgg
    27421 gatgcgctgg agatgagtgc cgaaatcacc gcgccctggt atcgactaca gctggacttg
    27481 ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc cgaagaccat cccaccgctg
    27541 gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt atgagcttat cgcctcgtta
    27601 aacaacattc tcaatctcta catgcctgcc gggcaggagg cagagcaccg ttttgcgatg
    27661 ggcgaactgc ctgatgaagt gctggagatc tgccagcggc tggcaaaact caccgagatg
    27721 ctgcgtggtc tggcggagtt atttcttaac gatttaagtg agaaaaccgg cagccacgat
    27781 attgtgcgtc tgcatcggtt gattttgcag atgaaccgcg cgttggggat gttcgaggcg
    27841 caaagcaaac tctggcggct ggcgtctctg gcgcaatctt ccggtgcgcc ggtgaccaaa
    27901 tgggcgacgc gggaagagcg cgaagggcag ctgcatctct ggtttcactg cgtggggata
    27961 cgtgtcagcg atcagctgga aaggctgctg tggcgcagta ttccgcacat tattgttacc
    28021 tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc aggagatgag tggtctgaaa
    28081 gagaaagcgg gcgaccgttt tgtggcgctg gattccccct ttaaccactg cgaacagggc
    28141 aaaattgtta ttccccggat gcgcgttgag ccttccatcg acaacgaaga gcagcatatt
    28201 gccgaaatgg cggccttttt ccgtaagcag gtggagagca aaaaacatct cggtatgttg
    28261 gtactgtttg ccagcggacg ggcgatgcag cgctttctcg actatgtgac ggatttacgc
    28321 ctgatgctgc tggtgcaggg cgatcagccg cgttatcgct tagttgaact gcaccgcaaa
    28381 cgcgttgcca acggtgagcg tagtgtgctg gtgggcttac agtcatttgc cgaagggctt
    28441 gatttgaaag gtgatctgct cagccaggtg catatccaca aaatcgcttt tccgcccatc
    28501 gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa gcctcaaccg ctatccgttt
    28561 gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc agcaggttgg gcgactgatt
    28621 cgaagccacg gttgctgggg cgaagtggtt atctacgaca aacgcttgct gaccaaaaat
    28681 tatggcaagc gactactgga tgcattaccg gtatttccga tagagcaacc ggaagtccct
    28741 gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac gccgtcggcg gcgttaatga
    28801 tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa tcattaaaga gatcgggcgc
    28861 gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc gcggtctgta tgcccatatg
    28921 ctcaatggtg aagtccctga ccttgagttg ggcggcgtat tgattgcgct acgtatcaaa
    28981 ggggaagggg aagcagagat gctcggcttt tacgaagcca tgcaaaatca caccatcaag
    29041 ctgacgccgc cagcaggcaa gccgatgccg attgtcatcc ccagttacaa cggcgcgcgt
    29101 aaacaggcca acctgacgcc gttgttggcg attctcctgc acaaactcgg ttttcctgtg
    29161 gtggttcacg gggttagcga agatccaacc cgcgtgctga ctgaaaccat ttttgaattg
    29221 atgggtatta cgccaacgtt gcacggcggg caggcgcagg cgaagctcga cgaacatcaa
    29281 ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg aaaaacaact ggcgatgcgc
    29341 tggcggatgg gcgtgcgtaa cagtgcacat accctggcga aactggcgac gccatttgcc
    29401 gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg aatacattgg acgcgtcgcg
    29461 aagttcttta gcgatatcgg cgggcgggcg ctactaatgc atggtactga aggtgaagtg
    29521 tatgctaatc cgcagcgctg cccgcagatc aatctcattg accgtgaagg gatgcgggtg
    29581 ctgtatgaaa aacaggaccc tgctggtagc gagttactgc cacaagcaaa agatccggaa
    29641 accacggcgc agtggattga gcgttgcctt gctggcagcg aaccgattcc cgaatcgctg
    29701 aaaatccaga tggcttgctg cctggtggct acgggtgaag cggcaactat cagcgacggc
    29761 ctggcgcgcg ttaatcaggc attttaattc ttttctcagc cggatgagcc atgtcatccg
    29821 gcttttaccc caccgcaata tgaaattcct gcatctttat tgaccttccc gcgcccggcg
    29881 tgcagcataa aaaaacaaca aacacataac ataaacagga gttaaccatg gaaagtggtc
    29941 atcgctttga tgctcagacg ctgcacagtt ttattcaggc tgtatttcgt cagatgggta
    30001 gcgaggaaca agaagcgaaa ttagtggccg atcatttaat cgcggcaaac ctggcagggc
    30061 atgattctca tggtattggc atgatcccaa gctatgtgcg ttcctggagt caggggcatc
    30121 tgcaaattaa ccatcatgcc aaaatcgtta aagaggcggg ggcggcagtc acgctcgatg
    30181 gcgatcgcgc attcggtcag gtcgcggcac acgaagcgat ggcgctgggg attgagaaag
    30241 cgcatcagca cggtattgct gccgtggcgc tacataactc gcatcatatc ggccgtatcg
    30301 gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc tatccacttt gttagcgtgg
    30361 tcggtattcc aatggtcgca ccgttccacg gtcgcgacag ccgctttggc accaatccgt
    30421 tctgtgtggt tttccctcgt aaagataatt tcccgctgtt gcttgattac gccaccagcg
    30481 ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg cgtccccgtg ccgccaggtt
    30541 gcctgattga cgttaacggc gtgccgacga ccaatccggc ggtaatgcag gagtcgccgt
    30601 tgggttcgct gttgaccttt gccgaacata aaggctacgc ccttgcagcg atgtgtgaaa
    30661 ttcttggcgg ggcgctttcc ggcggtaaaa cgacgcatca ggaaacgtta caaaccagtc
    30721 ccgatgccat tcttaactgc atgaccacta tcatcatcaa cccggaactc ttcggcgcgc
    30781 cggattgtag cgcgcagacc gaagcctttg ccgagtgggt gaaagcctcg ccgcatgatg
    30841 atgataagcc gattttgcta ccgggcgagt gggaagtgaa cacgcgtcgc gaacggcagg
    30901 agcaggggat tccattggat gcgggaagct ggcaggccat ttgtgatgcg gcgcggcaga
    30961 ttggtatgtc ggaagagacg ttgcaggctt tctgtcagca gttagccagc taaaaaaaag
    31021 cccgtccagt ggcggacggg caaacaaggg taacatagga tcaatgaggg ttagagcata
    31081 tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg acgtagcgta aacgcctgat
    31141 ccgtccaacg gttcggattt tgtaggcctg ataagacgcg gtagcgtcgc atcaggcatc
    31201 tgttgccgga tgcggcgtaa acgccttatc cttcctacga attacttata aattaccgca
    31261 gtaccgctta atttattgtt gttggtggcg gaagtgatgc tgtatccact ggcaccggct
    31321 gccgcggctt tctcagccag tttcgcttcc agggcatcga gggtggatgc gccatcggca
    31381 gaaaccacgc cgattttatt catgttctgt gcctgggatg ccgttaccgg ttccgcggca
    31441 aacacgccaa atgacagagt tgaaagagcc atagcagcaa caacagtatt gatagttttc
    31501 ataattaatc tctcgcaggt gatttttgta taagtgacgt tgtttcgtcg atgtgatgag
    31561 tatcacgttt ttttacgaga gataaaatcg aagagaattg accgccttgt tcaaataaat
    31621 tgattgatat ctaacatact gataaaccat ttatttgttg tacttatgga cttgttttat
    31681 gcagcgtggc agtcacgaca gcgttaaagg cacggaatgc cacgcaaaat acaaattata
    31741 ctcgctatac atctcgccag ttgtagttaa cgtaactggc tgtctttcga acctctgcga
    31801 ttatatcctg tatacgctgg tttttgtaaa tctttctcct gctgacaatg aatgcataag
    31861 cgcacgccag gaatggcttc ccgacgggct tgcgggatgg gggcaccgca ctcttcacat
    31921 tcatccaggc tttcgccacg cggaatttca ccccgagcgc gggcaatcgc atcttcaatt
    31981 gtactgttga tctgttcgtt gacggcgtca tcgttagccc aaccggatgc catatcgtcc
    32041 tccccatatc aatgcttgta cagttaagtg tagctaatcc agggacgaac ttgggcagtt
    32101 caagcatcag atctccgacc attcccgcag cagattatga taaagattaa gcagcgacag
    32161 gatctcttca ttttcaccat agcggctttt cagcgactga atattgttgt ccagttcaaa
    32221 cagcatggcg cgctttttat catcacggat catcgactgg atccacataa atgatgccac
    32281 tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg ctggagggat acaacacgag
    32341 atcgcctgcc gggagtttta cccgatgttg tccgaaggtg tcattaacga ccagttcgcc
    32401 gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca gaaaggtcag tacgcatcca
    32461 gccgttttgt ggatggctgc gtactgcgcc atccacatgg aaaccatagg tttcattgtt
    32521 ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc aaggccgcgg caaagaataa
    32581 agcatgttgg ttaaccgcgt tcagcacctc attttgcagg gcggcgtata acgtgctgcg
    32641 ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt gcgccggtgg tgacgcgtcc
    32701 atccacccat tcggcttgtt ccagttgttc gcgaaaccgg gcgacgtcct gtggcgataa
    32761 cacgccggga atgtggtaca tcatacaagt tctccaaaaa gtggggcctg cgccccacat
    32821 ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc ttggctcgcc cgggtgataa
    32881 cggtagccgc tcttgttgat tgaggcgacg taatcggtat caaacaggtt gtaaacgttt
    32941 agctggaagt cgagattgcg attaacccga taccccagtt tggcatcggc gacccagtaa
    33001 ccttcggtaa acgctggcgt tcccaccgcg ccgtctgaac ctttatgcat actgccgata
    33061 tagcgtgcgc ccgcgccaac agagatatca tctgttgcct gatactggct ccataaggtg
    33121 aaggcgtgct ccggggtata cggcagcgat gaggaaccat cctgggcaac atctttgccg
    33181 tttttgatgg ttgctttttg ctgggtatag ccgccaatca cctgccacgc gggcgtgata
    33241 ttcccggcca cggatatctc atagccttcg acacgtttct taccgtattg cgagtaagtt
    33301 ccgtcatcat tttgctcaac ttcattttcg atatcagtac ggaatagcgc ggcggtgagc
    33361 aacaggcgtt tatccagaac ctgccatttg gtgccaatct cgctggtgtt ggctttttgc
    33421 ggtttaaaat cggtgcggtt ggcactgtta ccgctgccag actgcgcaag ggcgaagttg
    33481 ttgccgcccg gaggctgctg ggaaacggca tagttaatat agacattacc gttttccgtc
    33541 aggtgataca gcgccccggc tttccagttc accagattgc ccgacttggc ggtgtcgacg
    33601 gtggtgaccg gagaaccttt tgccacacca gctgggcagg tgatggcacc gcgtccgctg
    33661 ccgccgcagg cggtggcact gtcatattca gtatgataat tatccagacg gatcccgccg
    33721 ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg cgtaaattgc gaaggtatcc
    33781 gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc cgggatgaat gctgctgtca
    33841 ggatgataaa tatttaccgc gggtaacgtc accggattaa cgccgtagtt agtctgcgtt
    33901 tcacgggtaa attccacgcc ggtactgaca tcatgaccga tagaaccggt atagaacgtc
    33961 gaggtcaggt tggtctggtt ggtgagaatt ttattactca catctttggt attcgccgtg
    34021 cgtgaccagg tccagctatt cacatcgctg gtgggctgag taatattcga cgccccgccc
    34081 ataatcgccg tcatcaggta atcctgcttt acgcgcgacc aacgggtagt attgcgaatg
    34141 gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg cggtgtcggt ggtcgaatcg
    34201 tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa cttttccgga atgattcaga
    34261 gccgctgttc ccgcagatgg ggcagaataa cccggcaaac cgatggtcgg aatgccgccg
    34321 tctggcgtgt tgtgctgggt gacatgcaga taattaagat acaaacgatt cgctgtacca
    34381 aggccaaaag cgatagaagg ggcgacgccg taacgctcat ttttgacttt gtcgcgtccg
    34441 gcatcgtgcg ttttttcgcc catcacattc aggcgcaccg cagtggtatc accaatgacc
    34501 tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc caatactggc ggaggcgtca
    34561 atgccggaat cattgcgcgg ctgcttgctg atcatattga tcgagcccgt cggcgcgctg
    34621 cgcccgtagt cggtgccgga aggcccttta atcacttcga cctgctcagt attgaaggtg
    34681 tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat aaatactgtt agaggtatca
    34741 gcaccgcgca tataaatggc gtcgccagtg gtggagttac cgttctcacc cgcaaaaaac
    34801 gcacccacgc cggggacgtt tttgagcgcg tcggtaaggt tggttgcgcc ctgatcttta
    34861 atcacttgtt cagaaatcac cgtcatcgtg cgggtagtat ccgctaccgg acgcgagaat
    34921 ttcggatcgg cagattgttg tggcgcataa agcgaaggcg ttgatgcttc gacaaccagc
    34981 gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct gagccacagg cgtgatgcca
    35041 atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc tggcagggaa attgcgattg
    35101 ttttccattt tgcaggtgac tttttttata tgttgaaatt ttcactgcca cgagaaagcc
    35161 tctctgcgga tgcgatgcca ctgttaatct cgtgatagtg ttcgtgcaag tgataataat
    35221 tttgataagc attatcactt ggcgcgattt tctatcaccc attgcgcgaa aaagaaatac
    35281 atttatttac attgcgttca tatttgatgt tttttgatta caaaacaggc gaaaactgca
    35341 tccagaggcg aatgcagtta cgttggtggg gttatttgta gatggttgca gtaccgaaca
    35401 tctggtcatt tccgcctgcg gaattaatga cataaccttt cgcgccttgc tcgcgcgctt
    35461 tttctgccag tttatcctcc agatcgctta ggtttgatgc gccagttgcc gaaacggtac
    35521 cggcgggccg tagctgaccg ctgtccaggt tactcatcgg ttgcgcggca taagccgtga
    35581 gagagattcc gctcaggacg gtggcaatca gtagtgtgag gcactttttc ataatgacat
    35641 cctcataaat acaacgggta gtgcctgata agtgtaggtc gcctgggcga tgaaaatgac
    35701 gcaaaaaatt gacagagatg tgcttatttt ttgaacgata tatttttaca aaataagaca
    35761 aatcaatgac atgcataatg ctgattttta tgatgaattg ccataaacca tagccatgtt
    35821 aaggttttgg ggaatgataa acgcagccag atatttaccc tgtcgcgtta tgcgggtatc
    35881 ttacgccgct gcttaaagga gaatgctatg tccgcccaga aaccggggtt gcatccgcgc
    35941 aaccgtcatc acagccgcta cgatctcgcc acgctttgtc aggtcaatcc tgaactcagg
    36001 caattcctca cgcttacacc cgcaggggag caaagcgtag actttgccaa tccgctggcg
    36061 gtgaaggcgc tcaataaggc attgctggcc catttttacg ccgtagcgaa ctgggatatc
    36121 ccggacggtt ttctctgccc gccagtaccg ggccgggcgg attatattca tcaccttgcc
    36181 gatttactgg cagaagcaag cggaacaatt ccagctaatg ccagcattct ggatatcggc
    36241 gttggtgcga actgtattta tccgctgatt ggcgtacatg aatatggctg gcgttttacc
    36301 ggtagtgaaa ccagcagtca ggcgttaagt agtgcgcagg cgatcatcag tgctaatccg
    36361 gggcttaacc gcgccattcg tctgcgtcgg caaaaagaga gtggggcgat ttttaacggc
    36421 atcatccata aaaacgagca atacgacgcg accttgtgta acccgccatt ccacgattcc
    36481 gccgctgcgg cacgggcagg tagtgagcgt aaacgccgta acctggggct gaacaaagac
    36541 gatgcactga actttggcgg ccagcaacag gagttgtggt gtgaaggtgg tgaagtcgcc
    36601 tttatcaaaa agatgattga agagagcaaa ggcttcgcga agcaggtgat gtggtttacc
    36661 tcgctggttt ctcgtggtga aaacttaccg ccgttgtatc gtgctctgac ggacgtgggc
    36721 gcggtgaagg tggttaaaaa agagatggcc caggggcaaa agcagagtcg ctttattgcc
    36781 tggaccttta tgaacgacga gcagcgccgc cgttttgtta atcgccaacg ttaaagcgtt
    36841 ggctcccccg gcggcagcgg ttcagccggg gccgcgcccg gagcaggcag cacctgataa
    36901 gtctgcactg gcgcgcgaac acctgccagg tcgaaatgct ttttcacctg gctgtcgagg
    36961 gcaaagcgga ccgtccactg tttgagtggc agcgtggtga acgaaacacg cagtgtaaac
    37021 gcggtattgc ttaagccgac aatcccggca aagtttggtt caccaataac cagcccgcga
    37081 atttcttcgt tttccatcaa ttccgctacc gcatctttca gtgcctgatt ggctttatca
    37141 gcatcttcat ggcgatcaac atcgtagttt gccaccaccg aaccaatgcc gcggacgaag
    37201 ttagcaaagg tggttatcga agaccaagga atgatgtgat acgccccggt atcctgacgc
    37261 acgcccacgg agcgaatcga catccgttcc acagtgccgg tcaacggccc gatagtaacc
    37321 aaatctccag tgttcatgcc gttttcaaac tgaataaata ccccggtgat aatatctttc
    37381 accagcgttt gcgaaccaaa cgagatagcc agacctaatg ccccagcacc tgccagcaat
    37441 ggcgcgatat tgacgccgat ttccgacaac acaatcatga tggtgatggt actgataatc
    37501 accgccagcg cgttacggaa cagcgtcagc agggtacgcg tacgggcgct gggtagcggg
    37561 cggccatgaa tatccgaagc cagccggttt tcgatcaaac tggcgagcac tgtccagcca
    37621 accgccgaga agaaaagaat gagtgcgatg cggatcagga tatctacggt tttctgcccc
    37681 gcgccgtttt gcagccagtt ccagaaatcg aacaatcccc atgcgctcaa cagcaacatt
    37741 accgccacgc agacggtcag aatacgcgcc gttttcagcg ccgccgaaag ccagccattc
    37801 agccgttttt gtagctccgg atagttacgc tgagtatgtg gcgagagggt gatggttttc
    37861 gccagccagc gggaaaacat accggaaaca aacgctgcga taccaataat cgccaggctg
    37921 cgcaccgttg cacccatcat aaatttcagg ctgttgcccg gatcgaacaa cgaaaagaaa
    37981 aacagcacga taaaataggc gcttgccagc cagtgccaca ccagcgcaaa ggcgcggata
    38041 aacaggctga aaaaggccag cgaatgctcc gcaaagttga gcaaatgttg ggtaatctct
    38101 tttttattgc gaaagatcag gtacaacgcc cagacggtca tgcacagcat aatgatgacg
    38161 ttcgccagcg cacctatctg tacattcacc tgattagaga taatcggcac ggccacaatc
    38221 aggccataac ctatcaaact gcttaaccag ctcaggcgac gactccagta acgggcgctc
    38281 tcgtcctgaa tcgtgaacgg gcgcagttca gccacgtttg ggcaaaaaat caggcgtagt
    38341 acggctttga aaaattcaat cagggcaaag gcgttgagaa acaaactttg ttggaaagcg
    38401 atggtgcgac tgcccgcatt caggttgtcg cttaatacct ggccgacaaa caatgtcagt
    38461 gccagtaaca gtaggtcgat aataaacgcc ccgataatca tcgccggaag ctgcagccag
    38521 ttgctgcgtt cgcgattttt ttgccgcgcc cactggccca ttttgcgata cagcggcagt
    38581 gcgcacagac gaatcagcca gtaaaaacca aacactaata ccgctaacat tgaaaaatgg
    38641 gtcaacgcat tgctgaaggt ttgtggatta aacggcttat gcggggagcc ggtgatattg
    38701 cgataaagtt gtccgaagcg ggcggaaagg gcttcaccat aatggcggct gacttcggtg
    38761 accttttgca gcacggtttg ctcttcaacc agcgtcggcg gcacgatctt cgggaccggt
    38821 tccgcagggg gcgtcgcggc gacagtacgc aactggtcga tcaattcttt acgcgaggtg
    38881 tcgttatcca gcacatccgc cagtgcgcca taggccgctt ttttttgttc gatatccggt
    38941 tccggggccg gttcagtcgt tgagtccgtt gtcgttgtgg ttgtaacgcc gggtatggat
    39001 accgcgtggg caggtgcgcc cagcaggcag aagaggatga acaggatcca ccgcatgact
    39061 cctccagtga gaaaacagag caaaaagata agtatagatg ctgggggagg ggcgatttta
    39121 aatgagagga atctggtgtg cctccctttc gggtgaaagg gaggcagaga agttaagaga
    39181 cgtgctgcaa aaattcctgc aagcgctggc tcggcgggtt cttgatcaat acctgcggat
    39241 cgccatcttc cgcaatccgg cctttgtcga taaagatcag ccgcgaagct actttctcgg
    39301 caaaaccgat ttcgtgggtc acgatcacca tcgtcatccc ttcttcagcc agatcctgca
    39361 taaccttcag cacttcatgg cgcagttccg ggtcaagagc ggaagtcggt tcatcaaaca
    39421 gcatcatttt cggcttcacc gccagcgcgc gggcaatcgc cacacgctgc tgttgaccac
    39481 cagaaagttc ggaagggtag tgatgtgcac gttctgccag accgactttc gccagcagct
    39541 cacgcgccag tttttccgcc tcttctttgt tcgcgccacg cacgcgtagc ggaccaaaca
    39601 tgacgttttc cagcgccgtc agatgcggga agaggtaaaa ctgctggaac accatacctg
    39661 cttcctggcg aatcaggcgc tcgtcaactt tcggatcgtt aaccttcagg ccatcgacaa
    39721 tcagatcgcc ggaggtgatt tcttccagtt tgttgatgca gcgcagcagg gtcgatttac
    39781 cggaaccgga cggcccgata atcaccacga cttcgccctg ggcaatgttc aaatcgatat
    39841 tgtgcagcac ctgggttggg ccaaagtgct tggagacgtt tttaaattca atcacaggat
    39901 tttcatcctt ctttccagac gacgcagaat aaagctcagc accagggtaa taatcagata
    39961 gaacaccgcc acggcgctcc agatctcaag ggcgcggaag ttaccggcaa taatttcttg
    40021 cccctgacgg gtcagttccg ccacgccgat cacaataaac agcgaggtgt ctttaatgct
    40081 gatgatccac tggttaccca gcggcggcag catacgacgc agtgccagcg gtaaaatgac
    40141 gtagcgaatg gtttcccaac gtgaaagacc gagcgccagt cctgcttcac gaaaaccttt
    40201 gtggatagac agcaccgcac cacgcgtgat ttccgcaatg tacgcgccgg agttgatcat
    40261 gatggtgacc accgccgcag taaatgggtc gatgcgtaag tcgttaaacg ccatcggcag
    40321 ggcgaaatag ataaacatca cctggacgac gataggtgtg ccgcggatca cttcaataaa
    40381 gaccagcgcg acgtggttgg ctatccaacc accgaaggtg cgtgcaaaac ctgccagcaa
    40441 tccgattacc agaccgcctg ccagaccgag gaccgaaatc cacagggtca ttttggcacc
    40501 ttcaatcaga agcggaatgg caggccagat ggcactccag tcaaactgca tatgttgttc
    40561 ctgttaccgt ggtgtaaata atcaaattca gggtaattaa tggccccgga gcgggtttgc
    40621 gcccctcacc ctaatcctct ccccataggg gagagggaac tgccagtgcg ttttacaggt
    40681 gtagcgttat tatttcggtt cagtaccgaa ccattttttg tagatttcgt tgtaagttcc
    40741 gttctcgcgc agggttttca acgcgccgtt gactttgtca cgcagctcgt cgctaccttt
    40801 cgggaacgca ataccgtatt gctgcgcttc cagagagtca cctaccgctt tgaactgacc
    40861 gttaccggcg gttttgatga agtacagaat gtttggcgta tcgtgcagaa cggcgtctgc
    40921 gcggttggtg cccagttcca tataggcgtt atcgatgttc gggaactgac gcagatcttt
    40981 agttttgatg tttgctttcg cgtaatcaac ggagccagta ccgctcttca cagcaaccac
    41041 tttcccgtcg agatctttca cgcttttcac atcgttattg ttagctttca ccatcactaa
    41101 caggccgctt ttgtagtagc cgtcagagaa atcgatcgct tttttacgct cgtcggtgat
    41161 ggtaatgccc gccagcgcca gatcgacgtt tttggtttgc agtgccggaa tgatcccact
    41221 gaaatccatc ggcttcagtt cgtaatccag cttcagctct ttagcgatgg cagcccacag
    41281 atcaacgtca aagcccacat atttatcgcc ctgtttaaat tcaaacggaa cgaaggcggt
    41341 atccgtcgcg acaactaatt ttttatccgc ggcatgagaa gaaaccgcaa aagccagggt
    41401 cagtgcagcc agtgaaactt ttaatacaga cttcatagca tttccttttt tctatccacg
    41461 ggacgatccc ctgcgtgaca ttcacatata tgaaaaaatc gtgccagttt tgcaactcct
    41521 tgatttttcg agatgcggca aaaacgtgat gcacgattta tagggcaata ccctgaagat
    41581 gcaccattct ggggcaccaa tctggtgcgc taaaattgtg cactcaacac agtgattatt
    41641 tagcgtaaaa cctgatgaaa aaacaatctt tatgtaacga ttgtgtgatg atgtggatac
    41701 aaaaaattta cttaatcagc tggagatagc agatggatgc actaaataag tgcgttgagg
    41761 tgggctgcaa aacaaaacgg cctcctgtca ggaagccgct tttatcgggt actaaagttc
    41821 tgcaccatca gcgatggatt tattcgatgt tagactcgat aaaccacagg aatttatcca
    41881 ggtcgcgaga cgcggcggtc aggatatctg cggtgtcgtc atctttcgct tcgccaatcg
    41941 ctttgcgtac gtcattagcg acgattgcgt aacggtcagc cagttctttc aggtgatcct
    42001 gaacgttgtg gatgtccagc gggtaacttt tcagcggggt tttgctgttg ataacttgag
    42061 tggtccccag agctacaccg cccagctgca ctgcacgttc tgccatggta tccagatgat
    42121 cgatcagtgc ggtgcggaag ccatccagca tttcatgtac ggcaatgaag ttagcgccgc
    42181 gcatgttcca gtgcgcttgt ttggtaatca aagaaagatc aataaactgg ataacctggc
    42241 gattcagcaa ctctactgtt gcttttttct cgctgtcgga gacatcgttg cgggtataaa
    42301 gcagattggt cgcttttgat ttaactaatt tagcggtact cataatttca tatcctcttg
    42361 atgttatgtc ccagtaatta acgagactaa gtatagcacc ggctatgtgt tccgctattc
    42421 tggctgttcc tatcacacta atagtggtaa caagcgtgaa aaacaaaact aataaagatt
    42481 caatgagtta gatatattga taagaacaat tcttatttat ctgctcgcca ggaattttcg
    42541 gaataaatat gcttttgcat aattcgatat ataaagacgg tgtagaggaa aagtagcgag
    42601 aaattctgca tggttatgca taaccatgca gaaatgtaaa tttaattaat gtctaattct
    42661 tttattttgc tctctttgcg tactgtcagc gtagacccca ttgaagcggc gatgatagcg
    42721 ccgagcgcca gcagctgtat gggtgtcagt gtttctccga ggaaaatcat cccggaaacg
    42781 gcagccagcg ccggttccat gctcatcagc gtaccaaatg tccgtgttgg caaacgggtg
    42841 agggcaatca tttccagcga ataaggcaga gcggtcgaga gaatagcgac agccagaccc
    42901 aatggaataa ccgaccagtg ccagagtgct tcaccagcct gaagcgctcc aattggcacg
    42961 aaaattaacg ctgcaatcaa cgaaccaatt gccaccgtcg cagggccatg ttccgctcct
    43021 gcgcgttgcc cacttaaaat gtaaatagcc caacaagccc cggcccccag tgccagcgca
    43081 cagccggtta aatcgacatg ggaaacgtct tgccccagcg gtagcaggaa ccacagacca
    43141 agaaccgcca gcacaaccca gacgaaatct accgggcgac gagaagagaa cagcgccacc
    43201 gccagtggtc cggtgaactc cagcgccacc gcaataccca gcggtactgt ctgaatagaa
    43261 agataaaaaa gataattcat cccacccagc gaaacgccgt aaaacaacag cggtaaccgt
    43321 tgctctttgg caaagcgcag tcgccatggc ttaaagaacg cgatgaggat cagcgttcct
    43381 aatgccagac gcagcgcagt gacacccggt gcgcccacca gaggaaaaag tgacttagct
    43441 aacgaggctc caccctgaat agacgccatg gcaacgagca atatgattat tggtaaccag
    43501 accggcattt tacgtaatga accaggcatc ctttctccca caaatatcta gacttaagta
    43561 aagcgtggag tgtactggat atacccaatg ctggttgagc atttgttgaa aaaattttcc
    43621 cccgttttga ctaaaatgcg ccaggattga tggaatcatt agtctggtga ttaggaataa
    43681 tctggatgaa tgacagggaa aacatgcgta atacttacgc agttctctga aaaagtgatt
    43741 taaatttaga tggatagcgg tgtatggaaa cgttctgtta catgaaatgg cccgttagac
    43801 atcacaaatc gcgaagagtt tcccattaat ttttgatata tttaaaactt aggacttatt
    43861 tgaatcacat ttgaggtggt tatgaaaaaa attgcatgtc tttcagcact ggccgcagtt
    43921 ctggctttca ccgcaggtac ttccgtagct gcgacttcta ccgtaactgg cggttacgca
    43981 cagagcgacg ctcagggcca aatgaacaaa atgggcggtt tcaacctgaa ataccgctat
    44041 gaagaagaca acagcccgct gggtgtgatc ggttctttca cttacaccga gaaaagccgt
    44101 actgcaagct ctggtgacta caacaaaaac cagtactacg gcatcactgc tggtccggct
    44161 taccgcatta acgactgggc aagcatctac ggtgtagtgg gtgtgggtta tggtaaattc
    44221 cagaccactg aatacccgac ctacaaacac gacaccagcg actacggttt ctcctacggt
    44281 gctggtctgc agttcaaccc gatggaaaac gttgctctgg acttctctta cgagcagagc
    44341 cgtattcgta gcgttgacgt aggcacctgg attgccggtg ttggttaccg cttctaatca
    44401 ctttggtgat ataaaaaatc cgcctctcgg ggcggatttt tgtttttaag gcttcgggtc
    44461 gaaaatatcg gttccgagat ggttgtaatc caccttctgt aactggaagt tggtgatgta
    44521 tatcgacccg gctttcttct cagatataaa cgggtatttg atgttaattt ccttcgcttt
    44581 aattcccgtc cactgggaga aaaaacctaa gaagtcattg gctgagcggc gggctttaat
    44641 cacccgatgc gctttatcgt cgctggaaat gaccataaaa ggcacctgga aattttgctg
    44701 atacttatca tcatgggcaa ggtattgcac gtctttgccg cgctctttaa aggccagacc
    44761 gtggtcagaa aagtaaacca gcgagaagct gctgccgctg ttgcgtaact gatcgtacag
    44821 cttgcgcagt aaatcgtccg tttgcgtcat ggtatagaga tagcacgacg tttctttcga
    44881 ctgcacaaag gtttcgtatt tgccttgtgt cctgtcgcag gcctgcggat gtgagcccat
    44941 cagatgtaga acaatcagct gcggttgcga gtgctcttgc gccagcactt gagcggtcat
    45001 atccagtaac gcttcgtctt tggtattttt atctgcttca aaattacctt ctttcaggaa
    45061 gtacacttca tctgctcgtt tggcgatgct ggcgatagcg gtatcgtatt cgccgatttg
    45121 accctggttg gaaaaccacc aggtctggaa gcccgcgcga tttgccaggg tgacaaagtt
    45181 atcctgaaac tgtggtttgc catcgactac gcgattgagc gttaagccaa gcgatttctg
    45241 cgtggagcca ctggcggcaa tgtagtcagc aaatatcaaa ccgttaacgc tgctggcaaa
    45301 cggggtattg tcccagtgac cgccaaaggc accgagggca tcgcgacgcg cgctttcacc
    45361 gatcaccacc acataggtct gatacttcgg cttaacggca gtgaccgtcc aggtatcttt
    45421 cattccggaa agttttgcca tccgttcttg ctcttcaatc acctcgttat tattgacgat
    45481 gacatcctta gcaaaacgaa ataccggata gccagtatct ttcagtttaa atacgccgcc
    45541 ccaggccagg ttttgcacgg gagcaacaaa aaatgtcgcc acactgaata ccagacacag
    45601 gctgtcgaag gtattccagc gcgctttttc actctctttt tttcgcctga ttgctattac
    45661 gccgagcaca aaaataaata tgccgaccat gtagctgtac cacggaaaaa tcgtcaggat
    45721 ttcggtcgac tcttccatat tggtggagtg caatgccagc aatgtattga aattaggcgc
    45781 gccgtaggcc tgagcaaaag ggaaataaca agccgccacc agcgaactga caccgaccag
    45841 aactttttgt acgcgaggca atgttcgcca tagcaaaagc aaaatagccg taaacgcagc
    45901 ggtgtagagc aaactgaagg ggtaacctaa gccgaggtta attaatagcg actgtaaaaa
    45961 gtagaacgca gtccacgggc taaatacccg gctacgggta acaagcgatt ctttgagggt
    46021 taaattcata tgccactatc gtaacaaacg ccatgtgctt accctggcgt caagggtcaa
    46081 tacctgcaag agtgcgtgaa gagtggaaag gggtccgcat ccgcgagccg caatatacgc
    46141 agggctgcaa gaagatagag cgagccgccg ttcaggtcaa ctactgacga acaaatcttt
    46201 tgcgaagggg attgcaaatc cgacaaaaag tggggttttt cagaccaaaa cgcgttatgc
    46261 ctgcatgaaa gcaggagtga agcggcgtac cgcgacgccg ctatttatcg gaaggtttat
    46321 cttgctgcgg tttgttgttg accatatcgc acaacataga gagcagcatt aaccgtactt
    46381 taaagggaga atgactaaac acgcgcatac acctcttgaa ctcattcata agacctcctg
    46441 acttgctaat cccgtcgatc cttgagggat gattgcatta catacagata tagcacaggc
    46501 tatattatat agctattgct aaaacgttaa ttttttgtac cttcgcaact ctggtttaca
    46561 atgtgcgcac gaaatgagaa cgctatgtat gcgtcaccat aatgaggaag cacaatgagt
    46621 cgtcgcgcag gtacgccaac agcaaaaaaa gtgacgcagt tagtgaacgt ggaagagcac
    46681 gttgaagggt tccgccaggt cagagaggcg catcggcgcg agcttattga tgattacgtt
    46741 gagctgattt ctgacttgat cagggaagtg ggggaagctc gtcaggtgga catggctgct
    46801 cgtctgggag tttcgcaacc gacggtggct aaaatgctta agcggctggc aaccatgggg
    46861 ctgattgaaa tgatcccctg gcgtggcgtg tttttaacgg cagaaggaga gaagctggcg
    46921 caggaaagcc gcgagcgaca tcagatagtc gaaaatttct tgttggtgtt gggcgtcagt
    46981 ccggaaatcg cccgtcgcga cgcggaaggc atggagcacc atgttagtga agagacgctc
    47041 gacgcttttc gtttgtttac ccagaaacac ggtgccaaat gagcctgcct tttttacgca
    47101 cgctgcaagg cgatcgtttt tttcagttat taattcttgt tggtatcgga ttaagctttt
    47161 tcgtgccctt tgcaccgaaa tcctggcctg ctgctatcga ctggcacacc atcatcacct
    47221 taagcggcct gatgctgctg accaaaggtg tggagttaag cggttatttt gatgtgctgg
    47281 ggcgcaaaat ggtgcgccgc tttgctacgg agcgtcggct ggcgatgttt atggtgctgg
    47341 cggcggcgct gctttctacc tttctgacca acgatgtcgc gctgtttatt gttgttccgc
    47401 tgactatcac gctaaaaaga ctgtgtgaga tcccggttaa tcggctgatt atttttgagg
    47461 cgctggcagt caacgctggt tcgctactga cgccaattgg caacccgcaa aatattctta
    47521 tctggggacg ttctggtctt tcgtttgccg gatttattgc ccaaatggca ccgctggctg
    47581 gcgcaatgat gctgacgctc ctgctcctgt gctggtgttg tttccctgga aaggcgctgc
    47641 aataccatac gggggtgcaa acaccggagt ggaaaccgcg gctggtgtgg agttgtctgg
    47701 ggctgtatat cgtctttctg acggcgctgg agttcaaaca agagctgtgg ggactggtga
    47761 ttgtggcggc aggctttgcg ctgctggcac gtcgcgtggt gctcagtgtg gactggacgc
    47821 tgctgctggt gtttatggcg atgtttatcg acgtccattt actgacccag cttccagcgt
    47881 tgcaaggcgt gttgggtaac gtgagtcatc tatctgaacc cgggttatgg ttaacggcaa
    47941 tcggtttatc gcaggtgatc agtaacgtgc cgagtaccat attgttgctg aactatgtgc
    48001 cgccgtcttt attactggta tgggcggtaa acgtaggtgg ctttgggtta ttacccggat
    48061 cgctggcaaa tttgattgcg ctacgtatgg cgaacgatcg ccgcatctgg tggcgtttcc
    48121 atctctattc aataccgatg ctgttgtgtg cggcgttggt gggatatgtt ttgttagtta
    48181 tactcccggc caactagggt ctggcaaata aaaaaggcgg attatgagtc cgccttttgc
    48241 tttaagtttt gaagattaat tcagacgaac cggcatcccg gagcggtttt taatcgcttc
    48301 atcaagaaca acctggtcaa catctggctg accggtcact gtttgcacgc tcttcgtcag
    48361 ggtaattggc acaatttcct gaccttcaaa ctgggcttcg gtggtagaca gcgggttatg
    48421 gacttcaata taacggctgc cgtctggctc ggtggtcgct tttaccggct catcaataaa
    48481 ctgtacgcgg gtaccgaccg gtactttctc gaacaggaat ttgatgtctt cgttacgcag
    48541 acgcacacaa ccatgactta cacgcaggcc gataccgaag ttggcgttgg tgccatggat
    48601 agcgtacagg cgaccgatgt agagtgcata cagccccatc gggttatccg gacctgccgg
    48661 aacgacagcc ggaagcggtt cgcccgcagc gcggtactct gcgtgcattt tggcggtcgg
    48721 cgtccaggtc gggcctgctt tcttacgctc aactttagtg gtccagttga taggcgtatc
    48781 tttgcctaac tgaccaatgc cgatcggcag cacgataacg gtgttggtcc ctttcggata
    48841 gtaatacaga cgcatctctg cactgttaat gacgatgcct tcatgaacgg tatccggcag
    48901 gatcagctgc tgcggaatgt tcagtacagt accgcctttc ggcaggaagg tatccacacc
    48961 cgggttcgct tccatcatat tggaaagccc catctggtac tccgcggcaa aatactccag
    49021 cggctgagtg ttaccttcag gaatggtgat cacctgattc tgaccaacca ggcgactccc
    49081 gtcggttggc agaggataag ttaccgcaga ggcggtactg caaaagccga caacagcgaa
    49141 ggccgctgcg aataatgttt tcaatttcat attcatgtta agcgagattt tgtgcctggc
    49201 aggccattgg gttgagaata ttagagtatt ggaagcgcat tataagttca ttccagctca
    49261 cagtgaaatc agatgtgtac gaaatcacat tttttgcctt tggcttgagt gtagacctta
    49321 agcgaggagc aggatcttct ttcagactta tggcataatg cgcggtttgt catatctctt
    49381 ttcaggatac gcctgtgtta gtttccagta acgtcaccat gcagttcggc agtaagccgt
    49441 tgtttgaaaa catttccgtc aaatttggcg gcggcaaccg ttacggcctg attggcgcga
    49501 acggtagtgg taaatccacc tttatgaaga tcctcggcgg cgacttagag ccgactttgg
    49561 gtaacgtttc cctcgatccc aacgagcgca ttggtaagct gcgtcaggat cagtttgcct
    49621 ttgaagagtt cactgtgctg gatacggtga tcatggggca taaagagttg tgggaagtga
    49681 agcaggagcg tgaccgcatc tatgctttgc cggaaatgag tgaggaagac ggctataaag
    49741 tggccgatct ggaagttaaa tacggcgaaa tggacggtta ctctgcggaa gctcgcgccg
    49801 gtgaattgtt gcttggcgtg ggaattccag tggaacagca ctacggcccg atgagtgaag
    49861 ttgctcctgg ctggaagctg cgtgtgcttc tggcgcaggc gctgtttgct gatccggata
    49921 ttctcctgct cgacgaaccg accaataacc tcgacatcga caccattcgc tggctggaac
    49981 aggtgctgaa cgagcgtgac agcaccatga tcatcatctc gcacgaccgt cacttcctta
    50041 acatggtttg tacccacatg gcggatctgg attacggcga gctgcgcgtt tatccgggca
    50101 actacgatga gtacatgacg gcggcgaccc aggcgcgtga acgtctgctg gctgataacg
    50161 ccaagaagaa agcgcagatt gctgagctgc aatctttcgt tagccgcttt agcgccaacg
    50221 cctcgaaatc tcgccaggca acttcgcgcg cgcgccagat tgataaaatc aaactggaag
    50281 aggtgaaagc ctccagccgt cagaacccgt tcatccgttt tgaacaggat aagaaactgt
    50341 tccgtaacgc gctggaagtg gaaggtctga ccaaagggtt tgataacggt ccactgttta
    50401 aaaatctcaa cttgctgctg gaggtcggtg aaaaacttgc ggtactgggt accaacggcg
    50461 tcggtaaatc aacgctgctg aaaacgctgg tgggcgatct gcaaccggac agcggcaccg
    50521 taaaatggtc tgaaaacgcg cgcattgggt actatgctca ggaccacgaa tatgagtttg
    50581 aaaatgatct gaccgtgttc gaatggatga gccagtggaa gcaggaaggc gatgacgagc
    50641 aggcggtacg cagcattctc ggtcgtttgc tgttcagcca ggacgacatc aaaaagccag
    50701 ctaaagtgct ttccggtggg gaaaaagggc ggatgctgtt tggtaagtta atgatgcaga
    50761 agccgaacat tctgatcatg gacgaaccga ccaaccacct ggatatggaa tccatcgagt
    50821 cactgaacat ggcgctggaa ctgtatcagg gcacgctgat ctttgtttca cacgaccgtg
    50881 agttcgtaag ctccctggcg acccgcattc tggaaatcac cccggaacgc gtgatcgact
    50941 ttagcggtaa ttacgaagat tacctgcgta gtaaagggat cgagtaagtg attatccgtc
    51001 accccgatac tgtcggggtg acggcggctg atgacaaatg caaaat
//
LOCUS       DAZGCS010000021        50122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGCS010000021 DAZGCS010000000
VERSION     DAZGCS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.021"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..471)
                     /gene="yebO"
                     /locus_tag="ACHK01_001325"
     CDS             complement(184..471)
                     /gene="yebO"
                     /locus_tag="ACHK01_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO8096301.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(546..689)
                     /gene="mgrB"
                     /locus_tag="ACHK01_001326"
     CDS             complement(546..689)
                     /gene="mgrB"
                     /locus_tag="ACHK01_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO8096302.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            848..1087
                     /gene="yobH"
                     /locus_tag="ACHK01_001327"
     CDS             848..1087
                     /gene="yobH"
                     /locus_tag="ACHK01_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO8096303.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1230..2021)
                     /gene="kdgR"
                     /locus_tag="ACHK01_001328"
     CDS             complement(1230..2021)
                     /gene="kdgR"
                     /locus_tag="ACHK01_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO8096304.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2198..3571
                     /gene="yebQ"
                     /locus_tag="ACHK01_001329"
     CDS             2198..3571
                     /gene="yebQ"
                     /locus_tag="ACHK01_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096305.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3617..4498)
                     /gene="htpX"
                     /locus_tag="ACHK01_001330"
     CDS             complement(3617..4498)
                     /gene="htpX"
                     /locus_tag="ACHK01_001330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO8096306.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4690..6738)
                     /gene="prc"
                     /locus_tag="ACHK01_001331"
     CDS             complement(4690..6738)
                     /gene="prc"
                     /locus_tag="ACHK01_001331"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO8096307.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6758..7456)
                     /gene="proQ"
                     /locus_tag="ACHK01_001332"
     CDS             complement(6758..7456)
                     /gene="proQ"
                     /locus_tag="ACHK01_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO8096308.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7553..8050)
                     /gene="msrC"
                     /locus_tag="ACHK01_001333"
     CDS             complement(7553..8050)
                     /gene="msrC"
                     /locus_tag="ACHK01_001333"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO8096309.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8180..9463
                     /gene="yebS"
                     /locus_tag="ACHK01_001334"
     CDS             8180..9463
                     /gene="yebS"
                     /locus_tag="ACHK01_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO8096310.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9432..12065
                     /gene="yebT"
                     /locus_tag="ACHK01_001335"
     CDS             9432..12065
                     /gene="yebT"
                     /locus_tag="ACHK01_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO8096311.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12145..13584
                     /gene="rsmF"
                     /locus_tag="ACHK01_001336"
     CDS             12145..13584
                     /gene="rsmF"
                     /locus_tag="ACHK01_001336"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO8096312.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13702..13938
                     /gene="yebV"
                     /locus_tag="ACHK01_001337"
     CDS             13702..13938
                     /gene="yebV"
                     /locus_tag="ACHK01_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO8096313.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            14043..14234
                     /gene="yebW"
                     /locus_tag="ACHK01_001338"
     CDS             14043..14234
                     /gene="yebW"
                     /locus_tag="ACHK01_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO8096314.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14235..14891)
                     /gene="pphA"
                     /locus_tag="ACHK01_001339"
     CDS             complement(14235..14891)
                     /gene="pphA"
                     /locus_tag="ACHK01_001339"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8096315.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15287..15628)
                     /gene="yebY"
                     /locus_tag="ACHK01_001340"
     CDS             complement(15287..15628)
                     /gene="yebY"
                     /locus_tag="ACHK01_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO8096316.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15641..16513)
                     /gene="copD"
                     /locus_tag="ACHK01_001341"
     CDS             complement(15641..16513)
                     /gene="copD"
                     /locus_tag="ACHK01_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO8096317.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQHEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16517..16891)
                     /gene="yobA"
                     /locus_tag="ACHK01_001342"
     CDS             complement(16517..16891)
                     /gene="yobA"
                     /locus_tag="ACHK01_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO8096318.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            17030..17260
                     /gene="holE"
                     /locus_tag="ACHK01_001343"
     CDS             17030..17260
                     /gene="holE"
                     /locus_tag="ACHK01_001343"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO8096319.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17362..18018
                     /gene="yobB"
                     /locus_tag="ACHK01_001344"
     CDS             17362..18018
                     /gene="yobB"
                     /locus_tag="ACHK01_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO8096320.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            18042..18704
                     /gene="exoX"
                     /locus_tag="ACHK01_001345"
     CDS             18042..18704
                     /gene="exoX"
                     /locus_tag="ACHK01_001345"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO8096321.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18701..20761)
                     /gene="ptrB"
                     /locus_tag="ACHK01_001346"
     CDS             complement(18701..20761)
                     /gene="ptrB"
                     /locus_tag="ACHK01_001346"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO8096322.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20970..21629)
                     /gene="yebE"
                     /locus_tag="ACHK01_001347"
     CDS             complement(20970..21629)
                     /gene="yebE"
                     /locus_tag="ACHK01_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8096323.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21956..22312)
                     /gene="yebF"
                     /locus_tag="ACHK01_001348"
     CDS             complement(21956..22312)
                     /gene="yebF"
                     /locus_tag="ACHK01_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO8096324.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22379..22669)
                     /gene="yebG"
                     /locus_tag="ACHK01_001349"
     CDS             complement(22379..22669)
                     /gene="yebG"
                     /locus_tag="ACHK01_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO8096325.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22803..23981
                     /gene="purT"
                     /locus_tag="ACHK01_001350"
     CDS             22803..23981
                     /gene="purT"
                     /locus_tag="ACHK01_001350"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO8096326.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(24037..24678)
                     /gene="kdgA"
                     /locus_tag="ACHK01_001351"
                     /gene_synonym="eda"
     CDS             complement(24037..24678)
                     /gene="kdgA"
                     /locus_tag="ACHK01_001351"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO8096327.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24715..26526)
                     /gene="edd"
                     /locus_tag="ACHK01_001352"
     CDS             complement(24715..26526)
                     /gene="edd"
                     /locus_tag="ACHK01_001352"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO8096328.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26761..28236)
                     /gene="zwf"
                     /locus_tag="ACHK01_001353"
     CDS             complement(26761..28236)
                     /gene="zwf"
                     /locus_tag="ACHK01_001353"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO8096329.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     YNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28574..29443
                     /gene="hexR"
                     /locus_tag="ACHK01_001354"
                     /gene_synonym="yebK"
     CDS             28574..29443
                     /gene="hexR"
                     /locus_tag="ACHK01_001354"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO8096330.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29571..31013
                     /gene="pyk"
                     /locus_tag="ACHK01_001355"
     CDS             29571..31013
                     /gene="pyk"
                     /locus_tag="ACHK01_001355"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO8096331.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31144..32115)
                     /gene="lpxM"
                     /locus_tag="ACHK01_001356"
                     /gene_synonym="msbB"
     CDS             complement(31144..32115)
                     /gene="lpxM"
                     /locus_tag="ACHK01_001356"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO8096332.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32235..33557)
                     /gene="mepM"
                     /locus_tag="ACHK01_001357"
     CDS             complement(32235..33557)
                     /gene="mepM"
                     /locus_tag="ACHK01_001357"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO8096333.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33573..34505)
                     /gene="znuA"
                     /locus_tag="ACHK01_001358"
     CDS             complement(33573..34505)
                     /gene="znuA"
                     /locus_tag="ACHK01_001358"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO8096334.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34584..35339
                     /gene="znuC"
                     /locus_tag="ACHK01_001359"
     CDS             34584..35339
                     /gene="znuC"
                     /locus_tag="ACHK01_001359"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO8096335.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35336..36121
                     /gene="znuB"
                     /locus_tag="ACHK01_001360"
     CDS             35336..36121
                     /gene="znuB"
                     /locus_tag="ACHK01_001360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO8096336.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36268..37278)
                     /gene="ruvB"
                     /locus_tag="ACHK01_001361"
     CDS             complement(36268..37278)
                     /gene="ruvB"
                     /locus_tag="ACHK01_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO8096337.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37287..37898)
                     /gene="ruvA"
                     /locus_tag="ACHK01_001362"
     CDS             complement(37287..37898)
                     /gene="ruvA"
                     /locus_tag="ACHK01_001362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO8096338.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(38037..38102)
                     /gene="yobI"
                     /locus_tag="ACHK01_001363"
     CDS             complement(38037..38102)
                     /gene="yobI"
                     /locus_tag="ACHK01_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO8096339.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            38173..38775
                     /gene="yebB"
                     /locus_tag="ACHK01_001364"
                     /pseudo
     CDS             38173..38775
                     /gene="yebB"
                     /locus_tag="ACHK01_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
     gene            complement(38777..39298)
                     /gene="ruvC"
                     /locus_tag="ACHK01_001365"
     CDS             complement(38777..39298)
                     /gene="ruvC"
                     /locus_tag="ACHK01_001365"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO8096340.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39333..40073)
                     /gene="yebC"
                     /locus_tag="ACHK01_001366"
     CDS             complement(39333..40073)
                     /gene="yebC"
                     /locus_tag="ACHK01_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8096341.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40102..40554)
                     /gene="nudB"
                     /locus_tag="ACHK01_001367"
     CDS             complement(40102..40554)
                     /gene="nudB"
                     /locus_tag="ACHK01_001367"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO8096342.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40672..42444)
                     /gene="aspS"
                     /locus_tag="ACHK01_001368"
     CDS             complement(40672..42444)
                     /gene="aspS"
                     /locus_tag="ACHK01_001368"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO8096343.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42754..43320
                     /gene="yecD"
                     /locus_tag="ACHK01_001369"
     CDS             42754..43320
                     /gene="yecD"
                     /locus_tag="ACHK01_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8096344.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43317..44135
                     /gene="yecE"
                     /locus_tag="ACHK01_001370"
     CDS             43317..44135
                     /gene="yecE"
                     /locus_tag="ACHK01_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO8096345.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DGRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44188..44583
                     /gene="yecN"
                     /locus_tag="ACHK01_001371"
     CDS             44188..44583
                     /gene="yecN"
                     /locus_tag="ACHK01_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO8096346.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44624..45367
                     /gene="cmoA"
                     /locus_tag="ACHK01_001372"
     CDS             44624..45367
                     /gene="cmoA"
                     /locus_tag="ACHK01_001372"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO8096347.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45364..46335
                     /gene="cmoB"
                     /locus_tag="ACHK01_001373"
     CDS             45364..46335
                     /gene="cmoB"
                     /locus_tag="ACHK01_001373"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO8096348.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(46500..48929)
                     /gene="torZ"
                     /locus_tag="ACHK01_001374"
     CDS             complement(46500..48929)
                     /gene="torZ"
                     /locus_tag="ACHK01_001374"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO8096349.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(48954..50054)
                     /gene="torY"
                     /locus_tag="ACHK01_001375"
     CDS             complement(48954..50054)
                     /gene="torY"
                     /locus_tag="ACHK01_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO8096350.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        11902 a        13023 c        12293 g        12904 t
ORIGIN      
        1 atcctaataa attaatgagg aaaatatctt tttctgatat cccgttacgc aaataaataa
       61 tatgataatt atttgaagtc agaccagagt tttaatttga agcagttgca ccatggcagt
      121 gcgtataatc aggatgaatt gagtaaccct tcaacgacgg ggttactcaa gattgaggaa
      181 ggattatcgt tcggcgacca gacgaataat atcttcgtct tcaaccgatt tttgactctc
      241 aacggtcttt ttatctgctt tctcaggttc gtttgcttcg cacaaacgac gtaacagtgc
      301 attttgacgt ttttgctgat ccagcaacgc ctcaagcagt tcaatctgtt cgttagtccg
      361 agaactggca cgattaataa aaaaccacaa gatgagcccg ataagaagaa ccaccaccga
      421 tacaaccaaa gacgcaatgt tcatcacgcc tgaatttaca acttcgttca tttcaccacc
      481 tctgagtaga ggcgctattc taccactgct ggagaggaag aaaatctagt gctgaaaaaa
      541 tgatatcacc acgggataaa ctggttaatg gcacaaattc cgctgaaaaa ttgtacatcc
      601 tgatcgcaca tcatgttgaa tacctgcgcc caaagcagca agcaagccaa caccacgaca
      661 accagaacga cccatcgaaa ctttttcact ccaatctccg tttcacctac cttatgtcat
      721 taaactagca tgcgttagtt aaacagcgcc taactatgtc gatattcgtg ctttttcgga
      781 atgagtcgct tgcttcagcg catattgccg ctacgattaa gcgaacataa aaagagaaga
      841 ggttgtaatg cgattcatca ttcgcacagt tatgctgata gcactggtat ggataggatt
      901 attactcagc ggatatggcg ttttgattgg aagcaaagag aatgccgcag gattaggttt
      961 gcaatgtacc tatctgactg ccagaggcac cagcacggta caatacttgc acactaaaag
     1021 tggtttcttg gggataacag attgcccact attacgtaaa agcaatatcg tggtcgataa
     1081 tggttaaggg taataaataa ccagattctc actcatgagt aaaatattct tatagcaagc
     1141 gcgtttaata taacgcacct tcaactttat ttttctcatg ctaaataaag aacctgtctg
     1201 tttgatattc cgacaggttc ttactctcat cagaacggat agtcgtgata acccatttgg
     1261 gcagaaattt tgcgcgctgc ggtatgcaac attgcgacat attcctgtaa acgctcttca
     1321 gagaaacgca acgtcgggaa ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca
     1381 aataccggta ccgcaatgca tcgcagccct tcttcctgct cttcgttatc ttcgccatac
     1441 ccctgctcgc gcacctggtc cagaacgggt aataacgctt ctgtactggt gatggtccgc
     1501 tcggtactgc gtttatactc cacgccctca agaatttgct tcacttcatc gagatcgcgc
     1561 catgccagca gtaccttacc aatcgcggtg ctgtacagcg gattacgacg cccaatccgt
     1621 gaatacatgc gcaaattgta catagagtca attttgtgta tgtaaacaat actgtcttcg
     1681 tccagtgcgc cgaggtggat agtttctttg gtcaggcggg agatctcacg catctggata
     1741 tctgcgctac gaattaaatc gacgttttgt aacgcgcgag cgcccagttc aaacaatttc
     1801 agggtcaggg aatatttctc cgactcccct tcctgcgcca cataacctaa ggttttcatg
     1861 gtctgtaaaa agcgataaac ggtgcttttt gacatcatga cgcgctgcga cagctcggtt
     1921 atccctattt cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa
     1981 gatacagaat caggctgttt atccagatct gcgttagcca tttatcacct cattgcgagt
     2041 gttttataaa aatcagaact gttttttatt ataatttcgc accagggtgg tcgcaatcca
     2101 tcttttgccg gttagttaca attctgcgac atccaccgtg aatatcagtg ctagaatcat
     2161 acccctgttg attattcacc aaagatataa aattcctatg ccaaaagttc aggccgacgg
     2221 cctgccattg ccccagcgat acggtgcgat attaaccatt gtgattggca tttcgatggc
     2281 tgtccttgac ggcgcaatcg ccaacgtcgc cctgccaaca atcgccacgg accttcatgc
     2341 cacgccagcc agttccatct gggtagtgaa cgcctatcaa atcgccattg tcatctccct
     2401 gctctcgttt tcgtttctgg gcgatatgtt tggctatcga cgtatttata aatgcggtct
     2461 ggtcgttttt ctgttgtctt cactgttctg cgccctttct gattcgctgc aaatgctcac
     2521 ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta ataccgcact
     2581 tatccgcctg atctatccac aacgttttct ggggagaggg atgggcataa actcgtttat
     2641 tgttgccgtc tcttctgctg ccgggccgac aattgctgca gcaatcctct ccatcgcatc
     2701 ctggaaatgg ttatttttaa tcaacgtacc gttaggtatt atcgccctgc ttctggcgat
     2761 gcgttttctg ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc
     2821 cgtgatgaac gcgttaacct tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg
     2881 gcaatcgctg acgttaattg ctgcggaact ggtggtaatg gttgttgttg gtattttctt
     2941 tattcgccgc cagctttctc ttcccgtacc gctgctaccg gtggatttac tgcgtatccc
     3001 gctgttttca ctttctattt gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat
     3061 ggtttccctg cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt
     3121 acttctgaca ccgtggccgt tagcaacgat ggtgatggct ccgctggcag gctatttgat
     3181 tgaacgcgta catgcaggat tgctgggtgc tttagggttg ttcatcatgg ctgcggggct
     3241 tttttccctg gttctgctgc ccgcgtcacc tgcggatatc aatattatct ggccgatgat
     3301 cttatgtggc gctggatttg gcttattcca gtcacccaat aaccacacca ttattacctc
     3361 cgctccgcgc gaacgtagcg gtggggccag tggcatgtta ggaacggctc gtctactggg
     3421 tcagagtagt ggcgcggcgc tggtggcgct gatgctaaat cagttcggtg ataatggtac
     3481 gcacgtttcg ctgatggctg cggctattct ggcagtgatt gctgcctgtg tgagtggttt
     3541 acgtatcact cagccacgat ccagggcata aaaaaagcgc gtcgatcagg acgcgctttt
     3601 tagtatttac ttcatattac ttcaggtatt cacccgtacg cagagcttca attcgtttat
     3661 ccagcggcgg gtgagtcatg aacaactcac tgagcgattt cgacttaccg ttaatgcaga
     3721 aagccatcat gctggttgct tcttgcggtt catagctggt tttcaggcgc tgcagcgcgg
     3781 caatcatttt ctcgcgacca accagttttg ccgaaccggc atcagcatgg aattcacgat
     3841 gacgcgagaa ccacatggtg ataatgctcg caagaatacc aaacaccagt tccagaaccg
     3901 ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc ttcaccttca tcacgatttc
     3961 cgcccataaa acccgcggca agctgcgcca gaatacggga aataaagata acgaaggtgt
     4021 tcaccacgcc ctgaatcagc gtcatggtga ccatatcacc attggcgatg tggctgattt
     4081 cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt ctgcagcaaa ccggtgctga
     4141 cagcaaccag agaggcatca cggcgcgcac cggttgcaaa agcgttgatg tccggcgcat
     4201 ggtagatagc cacttgcggc atagcgatcc ctgcctgacg agcctgggtt gctacagtat
     4261 taaccagcca acgttccctt tcgttacgcg gttgctcgat cacttccccg ccaacagatc
     4321 gtaatgccat ccatttggac atcagaagcg aaacgaagga accaccaaaa ccgaacagca
     4381 aggccatgat catcagcccc tgaacgctgc tcgactgtat ccctgtcagg ctcagtacca
     4441 gcccgaaaac gaccattacg gccaggttcg ttagcaggaa gagcgcgatt cgcatcataa
     4501 ttttcttttt acctcagttt aacaaaacgc aatatgcgat acccacatcg tatgggttac
     4561 gcgactattt tcaagtctgg atagtgcgta agtcaccaga aagacacaac tttacatttt
     4621 gtagcatctg atttacggca tcttgccgct gttaaaaaat caggcacaat ttcttgtgcc
     4681 tgattgatat tacttgacgg gagcgggttg ttccgcgggt ctggcttttt caagcttcgc
     4741 cagatcgagt gcgatattca ccgtctcatc cagataagga tccggctcct ggtaatcttt
     4801 aggtagatca tccagcttct tcaactccgg tttaccttcg cgtttaaagc gttcgttcaa
     4861 acgcgccaga cgcgtcgcat catcttcatt attctctttc tcacgcacag cgtagttcag
     4921 agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc gcgatatcct tcatgatgtt
     4981 ctggaactca ggatctttcg cgatacgcgc attatgttcc ttcagcagct ccggcccaaa
     5041 ggccgttaaa tcgcctgatt tcacataagt cgcggcatca atgctatccc acggcagcgc
     5101 gttatcttcg aatttctcac ccgtttccgt ttcttcatta cccgtcggca tgatgatgtc
     5161 tggcgttacg cctttacgtt gcgtactgcc gccgttaacg cgatagaatt tctggatcgt
     5221 gtactgcaca gaacccagcg ctggccattc aggacgtaac atctgatcgt aaatacggtt
     5281 caatgaacgg tattgctgaa cggtgccttt accaaacgtc ggttcaccca caaccagcgc
     5341 acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa gccgaagcac tgaaacggtc
     5401 aaccagtacc accagcgggc ctttatagaa cacctgcccg tcggtatcgc tgtcttcacg
     5461 aaccttgccg ttgttatcgc ggacctgaac aatgggaccc gcaggaataa acagacctga
     5521 gagcgatacc gcttcggtca gcgccccacc gccattgcta cgcaggtcga tgatcacact
     5581 gctgacattc tgtttttcca gtttctgcag ttgaactttg acatcgtctg tcaaacccac
     5641 atagaagccc ggaatatcca gcacgccgac tttctcttta ccgacggtct tcaccgacat
     5701 tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc aatgttacag tgcgggtctt
     5761 ggtcccttta ccagcaggta aaatttccag acgaacttta ctgcccttcg gccctttaat
     5821 taaggcaacc acatcatcaa gacgccagcc aatcacgtca accatcggct tgcctgtttg
     5881 accaacaccg acaattttgt caccaacgct gatagcttta ctcttcgctg ccggaccacc
     5941 tgccaccatc gaattgataa cggtgtagtc atcatccatt tgcagcactg cgccaatacc
     6001 ttccagcgac aaactcattt cagtgttgaa ctgttcggta ttacgcgggg aaagatagtt
     6061 ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc agcgagaaaa catcttcgct
     6121 gttggtttgc gccagacgac gaatggcaaa tttgtagcgg cgtgtcaggg tttcacgaat
     6181 ttctttatcc gtttttcctg ccagcttcag gcttaactcg tcgaatttga ctttactgtc
     6241 ccacagcgcg ttcaactcag cctcgttttt cggccagggc gctttgctgc ggtcaaggtt
     6301 ataagtgtcg ttgccggtga aatccatcgg cttttccagt accgacaaag cgtactggta
     6361 acgctcaaaa cggcgctttt gcgccagatt gtagagatcg tagaaaacgt cgagtttgcc
     6421 tgaacgcagt tcatcgccta actcggtttt ctttttcgcg aactgttcaa catcgcttgc
     6481 cagcagcacg ttgtggctgt aatcgagcag attcaggtag cggtcaaaga ttttggccga
     6541 aaatgcctga tcgaggtcga actggcgata atgagaacgg gtgaagcgcg acgttacgcg
     6601 ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc ggaatttgat cagcacgcgt
     6661 gatatcttct acagcgaagg tctggcctgc tattgcaagc aggccagcta acgcggtaag
     6721 cctaaaaaac atgttcatgc ctggcccggc ctccgtttca gaacaccagg tgttctgcgc
     6781 gcacaatcaa agacataccc gaattcagct ggacgcggac gccgtctttg gtgatttcta
     6841 atacggtggc atccatcgcg ttttgacccg ctttcacctt cagggcttgt ccgacagtca
     6901 gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg aggtgctttt actgtttttg
     6961 gcgctttctc taccggcttt tgcgcacgag gtttacgttc agcgccttct ttgcggcgtg
     7021 gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc ttctttctca ccagcagctg
     7081 cggcagcttc gcgttttttc gcttgctgtt cagcacgctg tgcctgaaca cgcgctttcg
     7141 cttcttcaag ctgcttgcga gcatgctcta catgttgctc gtccagctca ccgcatgggt
     7201 tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc gtaaagataa cgccagctcg
     7261 aagtgtagag acgtaaagcg gatcgtaatt gcgttttgct caggttcatt tccccggcaa
     7321 cacgatcgac caaatcctga aaaataccga ttttcagcgg acgcgcttca ccttccgcac
     7381 tgaaacagtg gggaaaacgt tcggccagaa acgcgattac ttctttactg ctattcaact
     7441 taggttgatt ttccatgaaa tttcctgatt acaacggacg tagccaacaa gcgcaggcat
     7501 gaacaggcgt cattataatg acgctaccag taaatgctac gttatccgtt gattatcctg
     7561 cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag cactttttca agctgtgcca
     7621 caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg accgaagacg gtactatcga
     7681 tgtcaagaac accaataatc tgatttttca ccaccagcgg cagaacaatt tcagaattac
     7741 tcgccgcatc acaggcaata tgcccgtcaa acgcgtgaac atcctcgata cgctgcactt
     7801 gattgcgggc aaccgcagtg ccgcacaccc cgcgcccgac gggtatccgg acacaggcaa
     7861 ttttgccctg aaatggtcct agtaccagtg tatcgtcctc aagcaaataa aaacctgccc
     7921 agtttacgtc agtgagacgc tcgtataaca acgcactggt gttcgcaagc gttgccagaa
     7981 aactggtttc tcccgccatc agcgcgttaa agtcgcgatt taaatccgcg taaaattctg
     8041 ttttgttcat tatataatca cttggttgtc ttacctggaa ctgccagcct attaaaataa
     8101 gcattaaatg cgttaatgct caagatcatt ccaatcatgg gttaatatta atgttaattc
     8161 ttattacatt tggcacgtca tggctcttaa cacaccacaa attacgccga caaaaaagat
     8221 aacagtgagg gcaatcggcg aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg
     8281 tgacatgctg tttagcctgc ccgagataaa ttctcatcag agtgcctatt gtccgcgctg
     8341 tcaggcaaaa attcgcgacg ggcgcgactg gtcgctaacg cgcctggcgg caatggcttt
     8401 caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt
     8461 aggtattcgt atcgacgcca acgtcatgca aggcatctgg caaatgacca aacagggcga
     8521 tacgataacg ggggcgatgg tctttttctg cgttattggt gcccccctca ttctggtgtc
     8581 ctccatagca tatttatggt ttggtaaccg actgggaatg aatttacgtc cggtactgct
     8641 gatgcttgag cgacttaaag agtgggtaat gctggatatc tacctggtcg gcattggcgt
     8701 tgcttctata aaggtacagg attatgccca tatccaggcg ggtgtcggct tgttctcttt
     8761 tgtggcgttg gtgattttaa cgacggtgac gttgtcacat cttaatgtcg aggagctgtg
     8821 ggagcgattt tatccgcagc gccccgctac gcgtagggac gagaaacttc gtgtctgtct
     8881 tgggtgccat tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc
     8941 gctacgcctg cgtcgccgtc atagtttgca aaaatgctgg gcggcgctgt tagcgtcaat
     9001 cgttttgttg ttacctgcca acctgttgcc tatttctatc atttatctga acggaggacg
     9061 gcaggaagat acaattcttt ccggaattat gtcgctggca agtagcaaca ttgcggttgc
     9121 gggaatcgtg tttatcgcca gtattctggt accgtttact aaagtgatcg tcatgttcac
     9181 tttactgttg agcattcatt ttaaatgcca gcaaggttta cgcacacgca ttctgttact
     9241 gcggatggtg acctggattg gtcgctggtc gatgctcgac ctgtttgtca tatctttaac
     9301 catgtcgctg attaatcgcg atcagatcct cgcttttact atgggaccgg ctgcgtttta
     9361 tttcggcgca gcggtaattt tgactattct tgctgtggaa tggctggaca gccgcttact
     9421 ttgggatgca catgagtcag gaaacgcccg cttcgacgac tgaagcgcag attaaaaata
     9481 aacgccgtat ctcacctttc tggctgctgc cgttcatcgc gctaatgatt gccggttggc
     9541 tgatttggga cagttatcag gaccggggta ataccgtcac catcgacttt atgtcggcgg
     9601 atggtattgt cccgggccgt acgcctgttc gttatcaggg cgttgaagtc ggaacagtgc
     9661 aggatatcag cctcagcgac gatcttcgta agattgaagt caaggtcagc atcaagtccg
     9721 atatgaaaga tgcgctgcgc gaagagactc agttctggct ggtgacgcca aaagcatcgt
     9781 tggcaggtgt ctccgggctg gacgccctcg tcggtggtaa ctatatcggc atgatgccag
     9841 gtaaaggtaa agagcaggat cactttgtcg cactcgatac ccaaccgaaa tatcgactgg
     9901 acaatggcga tctgatgatc cacctgcaag cccccgatct cggttcgctg aacagcggtt
     9961 cattggtcta tttccgcaag atccctgtgg gaaaagtcta cgactatgcc atcaatccca
    10021 acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa
    10081 aaggtagccg tttctggaac gtttccggcg ttgatgccaa tgtcagtatc agtggcgcga
    10141 aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc gattgccttc gattcacctg
    10201 aagagtcgaa acctgccgag gcggaagata cctttggtct gtatgaagat ctggcccaca
    10261 gccagcgtgg cgtaataata aaactggaac tgccgagtgg ggcaggatta accgccgact
    10321 cgacgccatt aatgtatcag ggactggaag tcggacagct aactaaactg gatttaaatc
    10381 ctggtggtaa agtcactggg gaaatgacag ttgatcccag cgtcgttacc ctgcttcgtg
    10441 aaaatacccg catcgaatta cgtaatccga aattatccct tagcgatgcc aatctcagcg
    10501 ccctgctgac tggcaaaacc ttcgagttgg tacccggcga tggcgagcca cgcaaagagt
    10561 tcgttgttgt gccaggcgaa aaagcactgc tgcatgaacc tgatgttctg acgctgaccc
    10621 tgaccgcgcc ggaaagttac ggtattgatg ctggtcagcc gctcattctt cacggcgtgc
    10681 aggtaggcca ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca
    10741 tcgagcctca gcatcgagaa ctggtaaaag gcgatagcaa atttgtcgtc aacagccgtg
    10801 tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg tgccagcgcc tcagaatgga
    10861 ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga gatgaaagcc agctatccac
    10921 tgtatgccaa tctggaaaaa gcgctggaga acagccttag cgatttaccc accacaaccg
    10981 tgagtttgag tgcagagacg ctgccggatg tgcaggcagg atcggtagtg ctctaccgta
    11041 aatttgaagt tggtgaagtt attaccgtgc gtccgcgagc taacgcgttt gatatcgatc
    11101 tgcatattaa gccggagtat cgcaaccttc tgaccagcaa tagcgtgttc tgggcagaag
    11161 gcggggcgaa agttcagctg aatggtagtg gcctgaccgt acaggcatcc ccgctctcca
    11221 gagcattaaa aggggctatt agcttcgata acctcagtgg tgccagcgcc agtcagcgta
    11281 aaggcgacaa acgtattctg tatgcttccg aaacagcggc ccgtgcggtt ggtgggcaga
    11341 ttacgcttca cgctttcgat gccggaaaac tggcggtcgg gatgccaatt cgctatctcg
    11401 gtattgatat cgggcaaatc cagacgctgg atctgattac cgcgcgcaat gaagtacagg
    11461 caaaggcggt gctctatccg gaatatgtcc agacctttgc tcgcggtggt acgcgcttct
    11521 cagtggtcac accgcaaatt tcggcagctg gcgttgagca tcttgatact atcctccagc
    11581 cgtatatcaa cgtcgaacca ggccggggca atcctcgccg cgactttgaa ttacaagagg
    11641 ccaccattac tgattcgcgt tacctggatg gcttaagcat tattgttgaa gcgccggaag
    11701 ccggttcgtt aggtattggt acgcctgtgc tgttccgtgg tctggaagtc ggtacggtta
    11761 caggaatgac gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc atcagtaaac
    11821 gctatcaaca cctggtgcgt aacaattccg tcttctggtt ggcatcgggt tacagtctgg
    11881 actttggtct gacgggcggc gtagtgaaaa ccggcacctt taaccagttt atccgtggcg
    11941 gcatcgcctt cgccacgcct ccaggtacgc cactggcacc gaaagcccag gaaggcaaac
    12001 acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga atggggaact gcgcttccca
    12061 aataatgccc actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac tgcgcgcctg
    12121 tttttttgcc agtggtacat gctcgtggcc caacacaccg tttatttccc ggacgccttt
    12181 ctgacacaaa tgcgcgaagc gatgccttcg acgctctcat ttgatgattt tcttgccgcc
    12241 tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc tgaaaatctc cgttgctgat
    12301 ttcctgcaat taaccgctcc ttatggctgg acgcttacgc caattccgtg gtgtgaagaa
    12361 ggtttctgga ttgaacgcga caatgaagat gcattgccat tgggtagtac cgccgagcat
    12421 ttaagcggcc tgttttatat tcaggaagcc agttcaatgt tgcccgttgc cgccttgttt
    12481 gctgacgata atgcaccaca gcgggtgatg gatgtcgcag ctgcgccagg ctccaaaacg
    12541 acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc ttgccaatga gttttccgcc
    12601 agtcgggtaa aagtgttaca tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc
    12661 acacattttg atggccgcgt gtttggtgcg gcagtgccag aaatgttcga tgccattttg
    12721 ctggacgctc cctgctctgg cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac
    12781 tggtcaccag aaagcaatca ggaaatcgca gctacacaac gggagcttat cgacagcgcc
    12841 tttcatgcat tacgtcctgg tggtacgctg gtttactcga cctgtacctt aaaccaggaa
    12901 gaaaacgaag ccgtttgcct gtggctgaaa gagacttacc ccgacgcagt agagttttta
    12961 ccacttggcg atctcttccc tggtgcaaac aaagcgctga ccgaagaagg ctttttgcat
    13021 gttttcccac aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag
    13081 gcgatccccg ccttacccgc ccccaaatac aaagtcggta atttcccgtt cagcccggtg
    13141 aaagatcgcg aagccggaca aattcgtcag gcggctgcaa gtgttggctt aaactgggat
    13201 ggaaacctgc gactctggca acgcgacaaa gaactgtggt tgttcccggt gggcattgaa
    13261 gccctgatcg gtaaagtccg attttctcgc ttggggatta aacttgccga aacgcacaac
    13321 aaaggttatc gctggcagca tgaagcagtt attgcccttg ccacccccga caatgtgaac
    13381 gcttttgaac tgacaccgca ggaagcggag gagtggtatc gcgggcgcga tgtttacccg
    13441 caagccgcgc cagtggcgga tgacgtgttg gttactttcc agcatcaacc gattggttta
    13501 gccaaacgga ttggttcgcg attgaaaaac agctatccgc gtgaactggt gcgcgatggg
    13561 aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag cgcacttttt gactggcaca
    13621 ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga ctggtcgtac cacaaccggc
    13681 agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac
    13741 gacggcgaat tacacggtga atcgccgggt gatcgaacgt taaccattcc ttgtaaatct
    13801 gaccccgatt tatgtatgca actcgatgcc tgggatgctg aaaccagtat ccctgccctg
    13861 ctaaatggcg aacactctgt cctttaccgt acccgttacg atcaacaatc tgatgcctgg
    13921 attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat ggcgggttat ttgtcctggt
    13981 tattcccccg ttgtaaaatc tctcctaaac ttaacggtac ggcaccacac ttcggggatg
    14041 aaatgttcgc gctggtactt tttgtttgct acctggatgg cggttgtgaa gatattgttg
    14101 tggatgtcta caacacggaa cagcagtgtc tttattctat gagcgatcaa cggatccgcc
    14161 atggcggttg ttttccgatt gaggatttta tagatggttt ctggcgacca gcacaggagt
    14221 atggtgattt ttaattattg caattgcaca agagtcagtt cgcccccaaa gacagcaccg
    14281 gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc gcaacggtgt atgaccaaac
    14341 cagaaatgat cagcacctgt aattccctgc cctttttggc gttcacctaa tcgcgagcgg
    14401 ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt cataaacatc atctggataa
    14461 tcggcatgag caataacatg tttgccggta cgactgtgta cttcaagaat aaagggcaaa
    14521 tgctgacatt tttccagcgc cgttttcgct tgtttctgtt gattatctgc cagcgcaata
    14581 aaccagtcgc cgccattcat caaccacaaa gacatctgct gggatgccag cgcatccatc
    14641 gccatctgtt catgattgcc tcttaccgca cgaacccaat gttgttccag taactgcagg
    14701 caacgtaaac tttgcggccc acgatcgata acgtctccca ctgagataag taaatctcgc
    14761 cacggatcaa aaccacaatg ccataatttg cggcgcaact gctcaagaca accgtgtata
    14821 tcgccagaaa gccagatatg tcgccattga tgacccgcaa ttctctgata aacgggcgca
    14881 ggctgtttca tcaatatttt cctcccgcgc taaagatcac ataatcttaa caagaatgtt
    14941 aaaaaacgct ggactcagac agtagagtgt gtgttatggt tgactataaa gtcagcgaag
    15001 gaaatgcttc tggcttttaa cagataaaaa gagaccgaac acgattcctg tattcggtcc
    15061 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
    15121 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
    15181 caataaaaag cgtggtggtc atcagctgaa atgttaaaaa ccgcccgttc tggtgaaaga
    15241 actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
    15301 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
    15361 tctgaatcac cgaaatgggt tgggctttag tcttcccact ggcaacttcc ttttgtgcga
    15421 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
    15481 gtcggcaagt cagcatcacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
    15541 aacggctgac ggtaatcacc tgcggtgcag ccagcgccgc tgcagaactg gtgagtaaca
    15601 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
    15661 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
    15721 actcaaacca gggtgccgca cgatcttcat gctgcctcat gcgcggtaca agaacataac
    15781 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
    15841 ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
    15901 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
    15961 gaatggcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
    16021 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
    16081 tcgccgttac cccttcattc agtgtcgcat gcccgattcc cgccagcaga ataaattgcg
    16141 cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca
    16201 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
    16261 cagcctgcca aatgtttggc gaaaatacat ctgcccatcc cgttcccatc aacccgccct
    16321 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
    16381 gcagaaaacg cttcgccagc aagcgacgaa tcgttaaggg ggccagccag gcaccgtaca
    16441 ttgcgaagcc aaagaccagc atcagcgagg tgaaatggat aaatcgcagc gcgatccagg
    16501 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
    16561 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
    16621 gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc cggtaatgtt ttaatatttt
    16681 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacacct tccgagaagt
    16741 ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
    16801 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg
    16861 cgtagcgaag ggagcgtgca gttgaagtca tattatctat tcctttttgt aataactttt
    16921 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
    16981 ctggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
    17041 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
    17101 ggtggcattt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
    17161 gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
    17221 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
    17281 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcacccat aatttgcagt
    17341 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
    17401 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
    17461 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
    17521 cgacgtgccc ttcctgcccc acccgatctc tcactgttag acccgctttg ttatgccgca
    17581 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
    17641 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggatctacca tcagagtcat
    17701 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
    17761 atggatatgg acccgacctg ttcactgttt actacagggc aatgtctggg agaacccgac
    17821 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
    17881 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
    17941 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
    18001 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
    18061 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
    18121 gaaaaatcgt caaccccatg agccacctgg tgcgccccga tcgtcctatt agtccacaag
    18181 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
    18241 tgatcccaca ctattacggt agtgaatggt atgtcgcgca taacgccagc tttgaccgcc
    18301 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
    18361 ggcctgggat caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
    18421 cgccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
    18481 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
    18541 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
    18601 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
    18661 cggagctgcg tttaacactg aaacattatc tggaaaatac ttagtccgca ggcgtagcgg
    18721 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
    18781 atttaaagcg accagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
    18841 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt
    18901 gcacctgaga atcgtgcaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
    18961 cgttgtcata tgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggattcc
    19021 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
    19081 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
    19141 caacgcccat caacatgccc cccgcactcc cgcccatcgc ataacaaagc gaaggagagc
    19201 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
    19261 tcttcagaaa ttttccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
    19321 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
    19381 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
    19441 gaaaatgttt gcgatggtag accaacgaaa ccggaacttc gacgccatca cgggcgacta
    19501 tccacaggtg ttcactgcgg taattcgccg catcaaaacc gggcacttct gtttgtttta
    19561 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtatctggt gtggtcatgg
    19621 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
    19681 cataggccgg atcatcaaag gcaataccaa tgacttcccg ggtcttgcgg ttaatttggc
    19741 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
    19801 acccttccag cataatgtta tcgcgtggcg gaattaactc ttcccactgt tgctcatcac
    19861 gcaggcgggt acggtataag ccaaagtttt tgccatggcg gttggaacgc agataaaacc
    19921 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
    19981 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
    20041 aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag gctgacgtaa taggtatcgt
    20101 ctttttcttc gtagatcagt ttatcttgcg atgctggcgt accgatggcg tgacgccaga
    20161 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
    20221 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttaccagttt
    20281 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
    20341 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
    20401 cgcgcttatt ggcatcgagc aatgtttccc actcatccca ctcttcactg aatgccgatt
    20461 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
    20521 ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg gtcgatgatt tcctttaaga
    20581 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
    20641 gcaggtagtc caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
    20701 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
    20761 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
    20821 agcgaaacag gggagtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt
    20881 atgcagacag agaaaatcag cctgttcaaa tggctgtgcg attctggata gcccgaaata
    20941 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
    21001 agatcgcgtt caatgccgtc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
    21061 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcaact caggaaatag
    21121 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
    21181 tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
    21241 tgctggtcga ttgccgcacg ttctttggca tcaatatgac catcactttt agcggcaaag
    21301 accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg gcgtactttg cgcgccaaac
    21361 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
    21421 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
    21481 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
    21541 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
    21601 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
    21661 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
    21721 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
    21781 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
    21841 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctgggg
    21901 cggacaaggc actcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
    21961 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
    22021 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
    22081 gcaaactgac ccaagccacg ggatcggcct gaccgacaat tttttgatca tctgcccaac
    22141 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gcagcatcca
    22201 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
    22261 atgcgcaggc agaaaccaac aacagcccta aaaacgcccc tctttttttc atgtttttct
    22321 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
    22381 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta
    22441 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
    22501 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca
    22561 gatccgccgt atcaagcatt ttatcataag catcggcttc ctttttgctg gtaaacgaca
    22621 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
    22681 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
    22741 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
    22801 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
    22861 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
    22921 ccgtcgatcg ctattccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
    22981 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
    23041 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
    23101 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
    23161 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc
    23221 ttttccgcga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
    23281 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
    23341 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
    23401 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
    23461 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    23521 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    23581 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    23641 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    23701 ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt cagtatggtc
    23761 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    23821 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    23881 gcagccgtcg tctgggggtg gcactggcta ctgcagagag tgttgttgac gccattgaac
    23941 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta
    24001 caaaaatgcc cgatcctcga tcgggcattt tgacttttac agcttagcgc cttctacagc
    24061 ttcacgcgcc agcttagtaa tgcgatcgta atcgcccgct tccagcgcat ctgccggaac
    24121 cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc
    24181 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    24241 cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta
    24301 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaattagag gaatagtccc
    24361 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    24421 cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc
    24481 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    24541 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ctgcgtgttc
    24601 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    24661 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    24721 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    24781 ttcacgtcct gtccccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    24841 cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat
    24901 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    24961 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    25021 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    25081 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    25141 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    25201 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    25261 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    25321 gagtgatttt tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag
    25381 ggtataacga gacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    25441 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    25501 ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa
    25561 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    25621 gttagtggaa ccaccggtcg ccagcagtgc aacgataccg ttcaccacca ctttctcatc
    25681 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    25741 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    25801 tggcaactgc atccccataa attccaccac catctggttg gtgttggcag taccgtagaa
    25861 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    25921 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    25981 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat
    26041 cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt
    26101 atgggacagc cccaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc
    26161 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    26221 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    26281 ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    26341 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    26401 gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata
    26461 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg
    26521 attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt
    26581 cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    26641 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    26701 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata
    26761 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    26821 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    26881 aatggagtct acccatttcc aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg
    26941 aataccacgc atggtttcca gcagcaaacg ttcataggca tccgccagat gcgtctgatt
    27001 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    27061 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    27121 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    27181 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    27241 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    27301 attgctgctc ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    27361 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttgtagc ggtcgatgcg
    27421 gcgcagagac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    27481 cggcggagac atcgcaatca tgcaaagaat ttgcagcagg tggttctgga tcatgtcgcg
    27541 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    27601 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    27661 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    27721 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    27781 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    27841 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt
    27901 gatacgattt ttttgatcca gcatcgcgcc gagacggctg aatgcagcag tgtcattgac
    27961 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    28021 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    28081 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    28141 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    28201 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    28261 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg
    28321 agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt
    28381 gcggcaaaac tgataaaaaa atcatcgttt tccattgggt aaaaatctga cactgatcat
    28441 gttatgaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    28501 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    28561 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    28621 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    28681 tattgctgct atggcactgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    28741 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaga gtctggcgaa
    28801 tggcactccc tatgttaatc gcaatgtcaa tgaagatgac agcgttgaat catacacagg
    28861 gaaaatattt gagtccgcaa tggcaaccct tgatcatgtc cgtcattcac tggataaatc
    28921 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    28981 aggctcttca gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    29041 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    29101 agacgtggtg gtgctgattt ctcacactgg aagaacaaaa aatctggtcg agctggcgca
    29161 gctggctcgc gaaaacgacg ccatggtgat tgccctcacc tctgcgggta ccccgctcgc
    29221 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    29281 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    29341 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    29401 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc
    29461 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    29521 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccagaa
    29581 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataacc
    29641 ttgaaaaagt tatcgcggca ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    29701 ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    29761 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    29821 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    29881 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    29941 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    30001 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    30061 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    30121 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    30181 tgaactatgc ccgtcggctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    30241 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    30301 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    30361 gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    30421 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    30481 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgcag
    30541 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    30601 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    30661 ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg gcgattatta
    30721 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    30781 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    30841 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    30901 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    30961 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    31021 gccggatgcg gcgaaaacgc cacatccggc ctacagttca atgatagttc aacagatttc
    31081 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    31141 tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg
    31201 agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc
    31261 gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc gcctctaaca gatcatccat
    31321 cggtgggcgc acctgaatcg tcagacggtg cgtcttgcca tcataaatcg gaaacagcgg
    31381 aacaacgcgc gcacggcaca ctttcatcaa acgaccaatc gcgggcaacg tcgctttata
    31441 ggtggcaaag aaatcaacaa attcgctgtg ctcagggcca tgatcctgat cgggtaaata
    31501 atatccccag tagccctgac gtaccgactg gatgaatggt ttaataccat catttctcgc
    31561 atgcagacga ccgccaaaac gacgacgcac cgtgttccag acataatcaa aaaccgggtt
    31621 gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg
    31681 aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat
    31741 ctcttcgatg atctccagcc cttgccagtc aacgcgcggc tgaattttct ccggcccgcg
    31801 tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac
    31861 aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc
    31921 acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg
    31981 atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacgc ccagccatgc
    32041 tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt
    32101 ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg
    32161 tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgacttc gtaccggctg
    32221 cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg
    32281 aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg
    32341 tttacggcct gctggtttat ccatacttca tagtgcaaat gcggcccggt tgaacgtccg
    32401 gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga
    32461 atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa
    32521 taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact
    32581 ggcgtaccct gcggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg accggtcacc
    32641 ggattagtac gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat
    32701 cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcttcagcg
    32761 cggatagcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc
    32821 tcacgtttac catcaagcat ttctcgagac attaataccg caaattcatc gccttttttc
    32881 agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg
    32941 gttaaaccgg cgtttctggc gctggcaaca aagctccccc cgacggtacc tttcagcaga
    33001 ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta
    33061 cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg
    33121 tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg
    33181 gcagcagcca gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt
    33241 agcgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca
    33301 tccagttcgt cctgaggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg
    33361 gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc
    33421 gtggcatcgc ggtgataaac atagggccgc cagacagcga cggccagagt aagaacggtg
    33481 agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag
    33541 cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac
    33601 tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc
    33661 cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc
    33721 gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt
    33781 cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca
    33841 agcggtgtca gtccaaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa
    33901 cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggctaattgt
    33961 gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact
    34021 aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc
    34081 atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca
    34141 tcatcgccgt gaatactttt catcagtagc ggtttcacat cttcaagctg cgcaatcgtt
    34201 acctgcttcg cttctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg
    34261 ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg acgcagtgaa
    34321 taatcatgtt ctgaagcgcc gtcaggcagt aaaacctccg tttctgttac accatcagca
    34381 atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc
    34441 tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt
    34501 aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg
    34561 actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt
    34621 cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt
    34681 tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc
    34741 tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa actgcgcatc ggctatgtac
    34801 cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac
    34861 gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccag gccgggcacc
    34921 tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctattagcgc
    34981 gagcattgtt aaatcgcccg caattattag tgctggatga acccactcaa ggcgtggatg
    35041 taaatggcca ggtggcgtta tatgacctta ttgaccaact gcgtcgtgaa ctggattgtg
    35101 gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt
    35161 gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt
    35221 ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata
    35281 accatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat
    35341 tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct
    35401 gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc
    35461 attacttggc gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtaattgc
    35521 cgttacgctg ctcctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat
    35581 tgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctgg tggtcgttag
    35641 tctgatgtct aacattcgtg ttgatttgat ggcttacctg ttcggtgatt tgctggcagt
    35701 gacgccagaa gatctcatcg ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt
    35761 ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt ttgttgatgg
    35821 tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg acggcattga cgattggtgt
    35881 agcgatgaaa tttgttggcg cgttgattat tacttcactg ctgattattc ctgcagctac
    35941 tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt tggtggggat
    36001 ggtggcagta actggcggtt taaccttttc cgcgttttac gatacgccgg cgggtccgtc
    36061 ggtggtgcta tgtgcggcac tgttatttat tctcagtatg atgaaaaagc aggccagcta
    36121 atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg
    36181 ctatctctgg taggccggat aagacgcgaa tagcgtcgca tcaggcacac tgccaatgtc
    36241 ggatgcggta cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg
    36301 attccacgcc cgcgtcgtcg ccatacgccc acgcggtgta cgctgcaaaa agccttgctg
    36361 aatcagataa ggctccagca catcctcaat ggtttcacgt tcttcgccaa tagccgccgc
    36421 caggttatcc agacctacag gcccaccaaa gaatttatcg attaccgcca gcaacaattt
    36481 gcggtccata taatcaaaac cttcagcatc gacattcagc atatccagcg cctgagcagc
    36541 gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag
    36601 cagacggttg gcaatgcgcg gcgtaccgcg agcgcgacga gcaacttcca gcgcgccgtc
    36661 atcactcatc tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg
    36721 cacctgataa aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag
    36781 cgaacctgcg cgtgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga
    36841 gcgtgccgcc ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccggata
    36901 caacacctct tcaacaactg gcgatagacg gtggatctca tcaataaaca gcacgtcatg
    36961 cggttcaagg ttagtgagca tcgctgccag atcgcccgcc ttttccagca ccggaccaga
    37021 agtcgtgcgt aaattaacgc ccatttcatt ggccacaatg ttggcaagcg tagttttacc
    37081 caaccccgga ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc
    37141 tttgatgaaa atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa
    37201 tttagggcga atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag
    37261 acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat
    37321 gtttcactgc tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt
    37381 ttatagccca gcgccaccag cgcggcaacc gcttcttgtt cagcatcgtc ggtcgccggg
    37441 ctggcaggag acgtgagcac cagatcggcg gctggcgtaa agagatcgcc gtgcaaacct
    37501 ttaaatcggt ctttcatttc gacaattaaa cgctcggcgg tttttttacc aatgcccggc
    37561 agtttcacca gtgctcccac ttcttcacgt tcaacggcat taacgaactg ctgcgctgac
    37621 attccggaga ggatcgccag cgccaacttc gggccgacgc cgttggtttt gattaactct
    37681 ttaaacaacg tgcgctcttg cttattgtta aagccgtaca gcagctgcgc gtcttcacgc
    37741 accacaaagt gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag
    37801 gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt
    37861 tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat
    37921 caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa
    37981 acttgcgagg cgtcccaacg atctgaaaaa tggcgcagta taatttattc tacaggttat
    38041 attggaagca aatatttgaa tattacatat tcagtgaaga aatgcgtaat aaaaatatac
    38101 attgcgactc ctgaaataat aaatttttta tgctattacg tatattcata tctatttcaa
    38161 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    38221 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    38281 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    38341 cccctttcaa ccatcaccac gctatctcgt tttattaaac gttcgtctaa tcaacgctat
    38401 gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag
    38461 gttccctccc ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag
    38521 ttctgttcaa aatttgtttt tgatatttat aaagaagcgc tatgcattcc ggtgggtgaa
    38581 atagaaacgt ttggagaact gttaaatagc aatccgaatg caaaactcac tttctgaaaa
    38641 ttctggttct taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg
    38701 catcatccgg gtttggtgtt gattcacgcg gagggagttg aaacgcctca gcctgaactg
    38761 accgaggcgg tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca
    38821 tctgcatcgc attctgacta acgtggcagt gggtgatggc aatcgccagt gcatcggcgg
    38881 catccgcttg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc
    38941 ttttctcggc gctaccaatg cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa
    39001 atactggcaa ctcctgattc accgccgcca caatcgccac gccgcgcgcc tggcccagtt
    39061 tcagggctga gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct
    39121 ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat
    39181 catccacttt ggtgcgtatg catccgctac ccaggtagga cagttgcctg cctacctggc
    39241 gaattacgcc atagccggtc acgcgcgaac ccggatcaat gccgagaata atagccatca
    39301 cgcgtctccg ttttgctgtt tagcaggcct catcagagag tcgctgcgac ctcatcagag
    39361 atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga
    39421 cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa
    39481 acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt
    39541 tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca
    39601 cctgcttcca gagctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg
    39661 ccttttttgc tgaacagata ggctacggaa ccatctgtac cgaggttacc gccacatttg
    39721 ctgaatgcat gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc
    39781 atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca
    39841 tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt catgttgtta
    39901 gacagtgctt tatccaccgc cgcacgcaga cgcgggttag cgtccggatc gccaccgccc
    39961 agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca
    40021 tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata
    40081 tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg
    40141 gttgctccag gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag
    40201 atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt
    40261 attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac
    40321 cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc
    40381 ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac
    40441 cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg
    40501 tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct ccacgctgcc
    40561 tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca
    40621 ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt
    40681 ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct
    40741 cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg
    40801 gatattgtcg gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc
    40861 gtgcggcgga gtaccgtatt tcagagcgtc gagcaggaag ccgaatttct cgcgctgttc
    40921 ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg
    40981 tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc
    41041 attttccggt gcagctttca gttctgcagc agtcatgtct ttcggtgagg tgaacggatg
    41101 gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca
    41161 cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag
    41221 tgcgcccatc gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc
    41281 atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc
    41341 taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag
    41401 acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag
    41461 cgatgcgccg cccggaacgc gcagagcggc aacgcgacct ttcggatcgt tcgccggacc
    41521 tgcaaatact gcaaactcaa cagatttcag cagatcagca acgtcggtca gttccatcgg
    41581 gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa aggtcattac
    41641 cgggaaatcg cccagatcca cgcccttcac ttccagccac agatggcgca ccagcgcttc
    41701 catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa
    41761 ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga tctgatagta
    41821 gcggtcaaag ccggacatca tcagcagctg tttgaacaac tgcggggatt gtggcagcgc
    41881 gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt
    41941 ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg
    42001 caccaggctg gtgattttag cgcgggtttt caggcgctga gccatttccg ggcgacgcag
    42061 gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg
    42121 cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcga tttcgccggt
    42181 cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat
    42241 gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa
    42301 aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc
    42361 acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag
    42421 ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc
    42481 ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg
    42541 cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc
    42601 gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg
    42661 cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt
    42721 gtgaggcacg attttgtttt ttcaggagtc attatgcttg aacttaatgc taaaaccact
    42781 gcgctggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact
    42841 gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag
    42901 cccgtgtttc tggtgcgcgt tggctggtct gccgattacg ccgaagcatt aaaacagccg
    42961 gttgatgccc cctcccccgc taaagtgttg cccgaaaact ggtggcaaca tccagctgca
    43021 ttaggtgcaa ctgacagtga tatcgaaatc atcaaacgtc agtggggtgc gttttacggt
    43081 acggatctgg agttgcaact gcgccgccga ggtatcgata caatagtgtt atgtgggatc
    43141 tcgaccaata tcggtgttga atccaccgcc cgcaatgcct gggaacttgg ctttaatctg
    43201 gtgattgccg aagacgcctg tagtgccgct agcgccgagc agcacaataa cagcatcaac
    43261 catatctacc cacgcatcgc ccgtgtgcgt agcgtggaag agatccttca cgcgttatga
    43321 tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc
    43381 ttgaagagta tgcccgccac tttaactgcg tggagggcaa caccacgctt tacgccctgc
    43441 cgaaacccga ggttgtcctg cgctggcgtg aacagaccac agatgacttc cgcttctgtt
    43501 ttaagtttcc ggcgaccatt tcgcatcagg cagcattacg acattgcgat gatttagtga
    43561 ctgaattttt gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac
    43621 tgcctgccac attcggccca cgggagctgc ctgcgctttg gcattttctc gattctctcc
    43681 ctggcgaatt taattatggt gtggaagtcc gccatccaca gtttttcgcc aaaggggaag
    43741 aggaacaaac tcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagacg
    43801 gccgcccggt tcatgcagca tgtccacaca gtgaagctat tcgcgacgct caacgaaaaa
    43861 aacctaaagt tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg
    43921 gtagtgatga tatgacgcaa aaccgggaat tatttcaggt ctggttacag aaattagcgc
    43981 agtggcatca gaccactacg ccttatcttt ttttacatac gccagacatt gcccaggccc
    44041 cggaactggt acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag
    44101 ttccggctat tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg
    44161 ccatcggcca ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg
    44221 ttgttattaa tgaagttctc ttttgatgtc gttcgcttgc gtttgcagta ccgcgttgcc
    44281 tatggtgacg gtggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg
    44341 gaatatattc ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc
    44401 tggatggtgc atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt
    44461 tttcatcacc gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg
    44521 ttgctgatgg tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt
    44581 tagcgcacaa tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac
    44641 gctattttct gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga
    44701 agtcttcccg gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat
    44761 tggtatgtta gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc
    44821 tctgggcgcg gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat
    44881 tgccatcgac aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa
    44941 agcccctacg ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc
    45001 atcgatggtg gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt
    45061 actggataaa atttatcaag gactgaaccc aggcggcgcg ctggtgcttt cggaaaaatt
    45121 cagtttcgaa gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg
    45181 tgccaacggt tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat
    45241 gctgaccgat tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag
    45301 cgagctgtgg ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc
    45361 tgcatgatcg actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg
    45421 ctcgaaacgc tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt
    45481 aagcagtggt ccaacgcggt ggaatttctg cctgaaatta aaccgtatcg tctggattta
    45541 ttgcatagcg taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt
    45601 gaaacgctta tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc
    45661 aacatcgata ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct
    45721 gatttaaccg gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc
    45781 atgattggcg caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc
    45841 cagtttgaag cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta
    45901 ggtattgaac agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt
    45961 taccatcgcc gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag
    46021 ggtgaactgg tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca
    46081 ggcgatcgtt acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa
    46141 aactggctga agaagtgtgg ttttgtagat atccgcgttg tggatgtgtg cgttaccact
    46201 actgaagagc agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac
    46261 ccacatgatc cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt
    46321 gcgcgcaagc cgtaaaggtc tggcattact gccggatgcg gcttgaatgc cttatccggc
    46381 ctacaaaccc ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg
    46441 catcaggcag ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct
    46501 tatgcctgaa tagctggctg atcaaacgcc gttaacttcg gcgcattgcc cgtatatttt
    46561 tcgatataca ccagcgcaga gttaccggca caggcatttg ccagctgcga gctggggata
    46621 tccgccgtta acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat
    46681 gcaccttcat gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc
    46741 agaatctgac cacgtttgtt ccacacacgc accagatcac cattcgcaat accaaagcga
    46801 gcagcatctt cggtgtgaat agtgattggt tcacgatctg caaccgcata ttttttacgc
    46861 agttccgcat agttaagctg actatgtaaa cggtgtgccg gatgtgcggt cagaagctgc
    46921 aactgcttct cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc
    46981 gggcaatcct tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt
    47041 ggcgtaccca gcgcattttt caccggatcg gcgcggaaat cagcataacg aacgtactgt
    47101 tcgttcttct cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac
    47161 atcggcatag tcacgcgttg cgcacgagca cctttctgag cggcatcata gaaaaatttc
    47221 agccacgcca tttcatcttt accttcggta tagatctctt ttccgccagg tttcagtaat
    47281 tcagcaagat cggcaaatac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc
    47341 tgcttcatcg gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc
    47401 tcaaacgatg tggtgatcgg taatacgata tctgcatgtt tagcagctgc ggtccagtag
    47461 cattcagaaa cgacgatcat ctccggtttc tgccatgctt tgatcagacg attagtatcc
    47521 tggtgatggg taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt
    47581 tgttctttac cgttatgctg atatttgcca ccaggatttt ccagcgcatc gacgatacgg
    47641 gcaacaggaa tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc
    47701 gccgcagaca tttccggcag cacgccgcca acacgcgtcg gattgccacc gttggagtaa
    47761 tgataagaga aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc
    47821 gtcaccagca tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagccagcc
    47881 atcagcattg tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt
    47941 acgccagtaa tttccgccgc ccaggccgcg ctcttcggag tattatcgct cttacctgtc
    48001 agatactctt caaactgcgg atacccggtg gtgtattttt caaggaacac tttatcgtgt
    48061 ttaccttgtg tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata
    48121 ttcggcgcga tccaggtagc attatcacca aagaactcga tggtttcgga gcggatagga
    48181 tcaatggcaa tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc
    48241 tcatcggtac tgctccaggc aatttttaag gtattcagcg ggttcatccc ccacagcacc
    48301 acagcctggc tgttttccag aatcagcggc caggaggtct gctgttcata cacctctaca
    48361 gaaccgacca catgcggcat gatcacctgt gcagccccgg ttgaataatc gccgctatgc
    48421 ccggaataac cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac
    48481 acgccagaag agcgccagcc gtatgatccg gcaaaaatag cagatggtcc gttagctttg
    48541 cgaatgcgat cgtgttgctc atgaatcagt tttaatgctt gctcccagct cacctgtaca
    48601 taagtatctt cgccacgacc tttcgccggt tgcagtggat tatcgagata gctttttctt
    48661 accatcggat gctgaatacg tgccgtggtg tgtacctgat ccgccgccgt agactgtaag
    48721 gagttcggta tggttttcgc cagcgcgcct gttgaagaaa caatcttgcc gtccttcact
    48781 tctacgttca tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc
    48841 catgcgggga gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt
    48901 ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt
    48961 ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt
    49021 tcgctcatgc tggttcgcgc acccattcct ttggcaatgg acggccatgc attgacggtg
    49081 taatggtcag cggcaatagg cgcatggcaa ccagcgcaat aggtatcgtc aagtttttca
    49141 gcgtattgcc atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa
    49201 gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc
    49261 gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag
    49321 agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca
    49381 ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga
    49441 gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc
    49501 agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta
    49561 tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat
    49621 tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag
    49681 tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac
    49741 ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt
    49801 aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc acattccgca
    49861 cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc
    49921 atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc
    49981 agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt
    50041 tttttccctc gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt
    50101 aaatacacaa cgaattaacc gc
//
LOCUS       DAZGCS010000022        49779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGCS010000022 DAZGCS010000000
VERSION     DAZGCS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.022"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..573
                     /locus_tag="ACHK01_001376"
     CDS             49..573
                     /locus_tag="ACHK01_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO8096351.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            657..1343
                     /gene="lpfB"
                     /locus_tag="ACHK01_001377"
     CDS             657..1343
                     /gene="lpfB"
                     /locus_tag="ACHK01_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO8096352.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALITTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNSNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     TGEMLTKPIN"
     gene            1367..3898
                     /gene="lpfC"
                     /locus_tag="ACHK01_001378"
     CDS             1367..3898
                     /gene="lpfC"
                     /locus_tag="ACHK01_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO8096353.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3909..4964
                     /gene="lpfD"
                     /locus_tag="ACHK01_001379"
     CDS             3909..4964
                     /gene="lpfD"
                     /locus_tag="ACHK01_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO8096354.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4970..5494
                     /gene="lpfE"
                     /locus_tag="ACHK01_001380"
     CDS             4970..5494
                     /gene="lpfE"
                     /locus_tag="ACHK01_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO8096355.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5747..7438
                     /gene="eptB"
                     /locus_tag="ACHK01_001381"
     CDS             5747..7438
                     /gene="eptB"
                     /locus_tag="ACHK01_001381"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO8096356.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7530..7606
                     /locus_tag="ACHK01_001382"
     tRNA            7530..7606
                     /locus_tag="ACHK01_001382"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7564..7566,aa:Pro,seq:cgg)
     gene            7641..7774
                     /locus_tag="ACHK01_001383"
     ncRNA           7641..7774
                     /ncRNA_class="other"
                     /locus_tag="ACHK01_001383"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8520..10127
                     /gene="dppA"
                     /locus_tag="ACHK01_001384"
     CDS             8520..10127
                     /gene="dppA"
                     /locus_tag="ACHK01_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO8096357.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10278..11297
                     /gene="dppB"
                     /locus_tag="ACHK01_001385"
     CDS             10278..11297
                     /gene="dppB"
                     /locus_tag="ACHK01_001385"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO8096358.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11307..12209
                     /gene="dppC"
                     /locus_tag="ACHK01_001386"
     CDS             11307..12209
                     /gene="dppC"
                     /locus_tag="ACHK01_001386"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO8096359.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12220..13203
                     /gene="dppD"
                     /locus_tag="ACHK01_001387"
     CDS             12220..13203
                     /gene="dppD"
                     /locus_tag="ACHK01_001387"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO8096360.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13200..14204
                     /gene="dppF"
                     /locus_tag="ACHK01_001388"
     CDS             13200..14204
                     /gene="dppF"
                     /locus_tag="ACHK01_001388"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO8096361.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGISFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14234..15505)
                     /locus_tag="ACHK01_001389"
     CDS             complement(14234..15505)
                     /locus_tag="ACHK01_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO8096362.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15981..16088
                     /locus_tag="ACHK01_001390"
     CDS             15981..16088
                     /locus_tag="ACHK01_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8096363.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16464..16571
                     /locus_tag="ACHK01_001391"
     CDS             16464..16571
                     /locus_tag="ACHK01_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8096364.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16947..17054
                     /locus_tag="ACHK01_001392"
     CDS             16947..17054
                     /locus_tag="ACHK01_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8096365.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17430..17537
                     /locus_tag="ACHK01_001393"
     CDS             17430..17537
                     /locus_tag="ACHK01_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8096366.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17624..19303)
                     /gene="bcsG"
                     /locus_tag="ACHK01_001394"
     CDS             complement(17624..19303)
                     /gene="bcsG"
                     /locus_tag="ACHK01_001394"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO8096367.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19300..19491)
                     /gene="bcsF"
                     /locus_tag="ACHK01_001395"
     CDS             complement(19300..19491)
                     /gene="bcsF"
                     /locus_tag="ACHK01_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO8096368.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19488..21059)
                     /gene="bcsE"
                     /locus_tag="ACHK01_001396"
     CDS             complement(19488..21059)
                     /gene="bcsE"
                     /locus_tag="ACHK01_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO8096369.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21332..21520
                     /gene="bcsR"
                     /locus_tag="ACHK01_001397"
     CDS             21332..21520
                     /gene="bcsR"
                     /locus_tag="ACHK01_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO8096370.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21532..22284
                     /gene="bcsQ"
                     /locus_tag="ACHK01_001398"
     CDS             21532..22284
                     /gene="bcsQ"
                     /locus_tag="ACHK01_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO8096371.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            22281..24899
                     /gene="bcsA"
                     /locus_tag="ACHK01_001399"
     CDS             22281..24899
                     /gene="bcsA"
                     /locus_tag="ACHK01_001399"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO8096372.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQIPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24910..27249
                     /gene="bcsB"
                     /locus_tag="ACHK01_001400"
     CDS             24910..27249
                     /gene="bcsB"
                     /locus_tag="ACHK01_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO8096373.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMLGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            27256..28362
                     /gene="bcsZ"
                     /locus_tag="ACHK01_001401"
     CDS             27256..28362
                     /gene="bcsZ"
                     /locus_tag="ACHK01_001401"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO8096374.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            28344..31817
                     /gene="bcsC"
                     /locus_tag="ACHK01_001402"
     CDS             28344..31817
                     /gene="bcsC"
                     /locus_tag="ACHK01_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO8096375.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31899..33887
                     /gene="hmsP"
                     /locus_tag="ACHK01_001403"
     CDS             31899..33887
                     /gene="hmsP"
                     /locus_tag="ACHK01_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO8096376.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            34070..35356
                     /gene="dctA"
                     /locus_tag="ACHK01_001404"
     CDS             34070..35356
                     /gene="dctA"
                     /locus_tag="ACHK01_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO8096377.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35577..37073
                     /locus_tag="ACHK01_001405"
     CDS             35577..37073
                     /locus_tag="ACHK01_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8096378.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(37169..38098)
                     /gene="kdgK"
                     /locus_tag="ACHK01_001406"
     CDS             complement(37169..38098)
                     /gene="kdgK"
                     /locus_tag="ACHK01_001406"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO8096379.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            38330..39097
                     /gene="pdeH"
                     /locus_tag="ACHK01_001407"
     CDS             38330..39097
                     /gene="pdeH"
                     /locus_tag="ACHK01_001407"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO8096380.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            39167..41227
                     /locus_tag="ACHK01_001408"
     CDS             39167..41227
                     /locus_tag="ACHK01_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8096381.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(41461..42783)
                     /locus_tag="ACHK01_001409"
     CDS             complement(41461..42783)
                     /locus_tag="ACHK01_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096382.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(43194..44207)
                     /gene="yhjD"
                     /locus_tag="ACHK01_001410"
     CDS             complement(43194..44207)
                     /gene="yhjD"
                     /locus_tag="ACHK01_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO8096383.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(44256..45155)
                     /locus_tag="ACHK01_001411"
     CDS             complement(44256..45155)
                     /locus_tag="ACHK01_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8096384.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            45675..46277
                     /locus_tag="ACHK01_001412"
     CDS             45675..46277
                     /locus_tag="ACHK01_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8096385.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(46328..47977)
                     /locus_tag="ACHK01_001413"
     CDS             complement(46328..47977)
                     /locus_tag="ACHK01_001413"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8096386.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            48382..49779
                     /gene="ccp"
                     /locus_tag="ACHK01_001414"
     CDS             48382..49779
                     /gene="ccp"
                     /locus_tag="ACHK01_001414"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO8096387.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        12121 a        12379 c        13495 g        11784 t
ORIGIN      
        1 ttgtatgaac aacatcaagc atatttatct atataaatgg agttataaat gaaaaaggtt
       61 ctgtttgcct tatctgcgct tgcgctgacc tctacttccg tattcgcagc tgatgcgggc
      121 gacggtactg ttaaattcac cggtgagatt gttgacgccg cttgtgttgt gtctccagac
      181 actcaaaaac aagaagttgt tctgggtcag gtgaacaagt ctgtatttac tgctatgggt
      241 gataaatcca cagcaacgcc gttcaaaatc aaactggaaa actgcgatat ctccactttc
      301 aaaaatgtgg aaatcagctt taacggcgtt ggcgatgcag acaacagcaa actggtttct
      361 gtaagcactg aaccaggtgc cgcaactggc gtaggtatcg gtatttatga taacaccaat
      421 accctggttg acctgaatac tggtaaatct gctactgtcc tgaaagaagg tcagaccgtt
      481 ctgtatttca ccgctaacta tgtctctacc aaagatgcag taaccacggg ttacggtaac
      541 gctgaagtcg acttcaacct gacttacaac taatcgatcc tgacggaacg atcatctctc
      601 aatggcaaca gagactgtgt tgccattatt cacaccaatg actcagggac cgaatcatga
      661 aacgtattag ctttgctgtg ctggcacttt taatgaccgc acaacagtgt tttgctggtg
      721 gcgtagcctt aatcacgacg cgtgttattt atgatggcgg caagaaagaa gcgtcgctga
      781 ccgttgaaaa ccataataaa aatgaagagt ttttgatcca gtcatggatt gatgatgtga
      841 atagcaataa aaaaacaccg tttattatta caccgccatt attcaaactt gatcctgata
      901 aaaataacgt cttacgtatc gtcaatattg aagatacctt acctaaagat cgtgaatccg
      961 tttactggat taatgtaaag gccattcctg cgaaaagtgc tgaaaatgaa aataaaaacg
     1021 tcttacagat agccgttcgc acccgtttga agttgttcta tcgtccaaaa ggtttgccgg
     1081 gaagcaacct cgaggccatg aaacagttac gtttccagcg tcaaaataac caaattgtgg
     1141 ttaataacga ctccgcttat aacctgacct ttaatcagtt ttccattaat ggacaaaaga
     1201 ttgaacgggc gggaatggtt ctcgcgaaag gaaagttaaa cattaacctt ccagccggta
     1261 caggcaacgc gcacgaagtg aaatacagca ttattaatga tttcggtgtt actggcgaaa
     1321 tgctgacgaa gccgattaac tgatcctttt acaaggcgct tttactatgc attggatgca
     1381 tttacctctc tatcactaca gagcgcattt ttccttctct ctgctggcat taacgattgc
     1441 cagctcgtta cctgcgtatg gcggaaaatt taaccctaaa tttctcgaaa acgttcaggg
     1501 tatcgatcaa cacatcgact tgtcggtata cgactcacct gtcggccagc aaatccccgg
     1561 taaataccgg gtttcggtgt ttgtaaacga agaaaaaatg gcttcccgga cgctggattt
     1621 tagtaccgcc agcgaagcga aacgcaaagc cagcggtgag tcgcttatgc cttgccttag
     1681 ccgggtgcaa ctggaagaaa tgggcgtgcg cgttgacagc tttccggcgc tgaagatgtc
     1741 accaccagaa gcctgcgtag cgtttgatga aatcattccc caggccacca gccgctttga
     1801 ttttaatact cagacgctgc atctttcctt tccacaggcg gcaatgatga tgaccgctcg
     1861 cggcacggtt gatccttctc gctgggacga aggaatacct gcgctgttgc tggattacag
     1921 ctttagtggc agcaatggac gcaatgaagg tacaggttca tcttcagatt cgaccagtga
     1981 cagttattac ctgaacctgc gcagcggttt gaacgttggt ccgtggcgtt tacgtaataa
     2041 cagtatctgg aaccgtaccg atggcaaaaa ccaatgggat aacgttggta catcgttaaa
     2101 ccgggcaatt attccactta aatcacagat aactctgggt gataccgcca cgcctggcga
     2161 gatttttgat agcgtacaaa tgcgtggggc gttactggca tccgacgatg aaatgttgcc
     2221 ggacagccag cgtggctttg ctccggtagt gcgcggcatc gccaaaagca acgctgaagt
     2281 gagtatcgaa cagaacggct atgtcattta tcgcactttt gttcagccgg gtgcatttga
     2341 gattaacgat ctctacgcaa cctccggcag cggcgacctg acggtgatca ttaaagaagc
     2401 cgacggaagt gaacaacgtt ttatccagcc gttttcggcg gtggcgattt tccagcgtga
     2461 agggtatctg aaatacagtc tggctgccgg tgaatatcgt gctggtaatt acgacagcga
     2521 taaaccacat ttcgggcagt ttaccgccat gtacggcttg ccgtggggta tgaccgccta
     2581 cggtggtgca ttgttgtcgg cagattacaa cgctttagct ctgggcctgg ggaaaaactt
     2641 cggtaccatt ggcgcagtat cggtggatgt cactcaggcg aaaagtcagt tacgcaacaa
     2701 tgaaaaagag gaaggccagt cttatcgctt cctctattcg aaaagttttg agggtggcac
     2761 tgacctgcgt ctgttgggct acaaatactc aaccagcggc tactacacct tccaggaagc
     2821 aactgatgtt cgcagtgacg ccgacagtga ttaccgccgt tatcacaagc gcagccagat
     2881 acagggcaat attacccagc aactgggaga ctacggctcg gtttatttca atatgacgca
     2941 gcaggattac tggaacgttg acggtaagga aaactcctta agcgccggtt atcacggtca
     3001 cattggtcgg gtgaattaca gcgtggccta tacctggacg agaagtccgg aatgggaaga
     3061 agacgatcgc ttgtggtcat tcagtgtttc catccctctg gggggcgcgt ggagcagtta
     3121 tcgcatgact accgaccaga atggcaagac gtcgcaacag gcctctgtta gcggcacgtt
     3181 actggaagat cgtaacctga gctataacgt tcagcagggt tacaccagta atggcgtagg
     3241 ctacagtggc agcgtcaaca tggggtatat gggtggctct ggtaacattg acgtaggcta
     3301 taactattct aaagataacc aacaggttaa ctatggcgtg cgcggcggtg tcatcgttca
     3361 cagtgaaggt ataacgcttt cgcagccttt aggcgaaagt ctggcgattg tttctgcacc
     3421 gggcgcgcgt ggtggacatg tggttaacag cagcggcgta gaagttgact ggatgggcaa
     3481 cgccgtggtg ccttatctca caccatatcg tgaaaccatt gtggaattgc gtagcgacac
     3541 gttggggcaa aacgttgaac tacaggaagc gttccagaaa gtcgtgccta ctcgtggtgc
     3601 agttgtgcgt tcccgctttg atacccgcgt cggttaccgt gtgctgatga gcttaaaaca
     3661 ggcgaatggc aacgcagtac cgtttggtgc aaccgcagcg ttaattgatg agagcaaacc
     3721 tgccagcagc attgtgggtg aagaaggaca gctttatatc agcgggatgc cagaagaggg
     3781 cgaattgcag gtgagctggg ggaacgagca ggcgcaacgt tgccgtgtgc cattccgctt
     3841 gccagagaac aaggacaatg cggctatcgt gatggttaat gcggtatgcg agaaataagg
     3901 atatttagat gaaaattaaa aatttgttgg caggtatggt gctgttaggg acgagcactt
     3961 ttgctggaaa tatatgggct gcggattggg gaccatgtca gacatcaaat ggggtggccc
     4021 atgagtatag ttttgatttc gtacagacta tccaggtacc ttcagaaaat aaggctggga
     4081 aaatattaac tcagcccttt gctttaggta caaaatattc cgcttattgt gaatgcccgg
     4141 atcccattcc agataatgga gttgtgactt attttaaagg tgtaacgctt ctgcctgagc
     4201 ctggaactgt agatgggtac tataaattca ataattacat tgatattctc accaaaatat
     4261 atgtctacac acaattagat atcccggtgc cttttaccga tcgaagcaat ggcacggcac
     4321 agaaagaatg tacgccctat actgctaaca attgggggac aggtggtaaa ggaagtatca
     4381 gtatttatat ttcacatcct tttgttgggc aaatgattat tcctaaaaca cgagttgcgt
     4441 ccctctttgg gacaaaaaag aaaggtgttt acaatgatag tcagcccatg gccaatgtca
     4501 gtatttccgg gagtattaca gttactcaag gctgtgaact tgccgctggc accgtactgg
     4561 atattccatt cggagaatat caggcccatg attttaaagg gcgtgccgga caaccaccac
     4621 aaaatgttca gaaggtacaa aaagaattaa gctttgactg caataatatt tcagatggcg
     4681 tcaaaattta tctcagtatt gatgcaacac caaatactgc ctatcccagc gcaattgatt
     4741 taggcaacgc cgatgttggt gcagtcattg aggacggtaa aggtaatatt cttaagccga
     4801 atgacagtaa tagcttatta gaaatgaacc caggctcgct gtatgaagat gtcaaacgca
     4861 aagcgacaac cacaattacc gcttatcccg ttagcactac cggaaaatta cccgctgccg
     4921 gggattatag cggtattgct accatacatg tcgaactgga gtaacagtca tgaagcgaac
     4981 aatacttgcg ttatgcctgc tgcctctttc cttttcgtca ttcgcggcaa ccacggattt
     5041 agggccgaaa ggaacattga aattcaccct taaaatcagc cagggtgctt gcgaactggt
     5101 gaaagacagc gttgaggttg acatgggcac cgcggttctc aaaaagccgg taagaatcgg
     5161 aacggaaatt aatcccactc ctttttccat cggcctgaaa aactgtagcg aagtggtcag
     5221 agcctacgtg actatggacg gtacacccaa tgcagacgac tcaaatcttt ttgcgctgga
     5281 tgcgggtggc gctacaggcg ttgggctgaa aatcaaaacc gctgctggag tacaacagtt
     5341 cccggaaaat acaacgccga caccggttga gtttgcaatt aacggtgatg gcgcacatca
     5401 actgaactat atcgccagct acgtgcctat tcgcgccgat gcaactattg gtcgcgcaga
     5461 tgcaacggtc gactttactg tacagtacga ataatttcac atcttaaggg ccagttaact
     5521 ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca tttttgtaaa
     5581 tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt ctacttattc
     5641 gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg ggcctgaaaa
     5701 gccgctaagc agggtgttat cacctgtttg tccagggttt gtttgcatga gatacatcaa
     5761 atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc tttttatgaa
     5821 tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg tctggaaagg
     5881 cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt tactacgtct
     5941 tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg tgctcttttc
     6001 cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg gcatcatcgc
     6061 ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga actttattct
     6121 ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc gctgtcgcta
     6181 caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt
     6241 actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc agaagaaagt
     6301 ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga actcttatct
     6361 gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg atgaatcttc
     6421 cgataataat tcattgctta atccggcgaa gaaattcact tatcaggcac cgcaaaacgt
     6481 tgatgacact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc atatgggcat
     6541 tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc tggcggcgtt
     6601 ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt ttgtacgtca
     6661 ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt tcgcggttct
     6721 gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt ggttctacag
     6781 caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc cacgtaatcg
     6841 tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc tagggcgcaa
     6901 cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta actacaccca
     6961 gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg atagcggctg
     7021 taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg atcactttat
     7081 ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg cagctgacca
     7141 cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc
     7201 ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg aaaatccggc
     7261 caatgctcag gcatttgcgc agctgaaaaa agaagccgac atgaaagtgc cacgccgtca
     7321 tgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg atggtggaat
     7381 taacgaaaac aacaactggt gtcacatccc gcagacaaaa gtggcagcgg ctaactaagc
     7441 ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt gacgagggcg
     7501 tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcctggt agcgcacttc
     7561 gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt cgaaaagcct
     7621 gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct ccgggggcgg
     7681 ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg cagggcgagc
     7741 cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc tcaacgagca
     7801 ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcgggg aggttcgact
     7861 cgacgtagat actgataaga cgcgccagcg tcgcatcagg caacggcacc gcacttattg
     7921 tcggatgcgg cgcgaccgcc ttattcgacc tacacaccac acatttccct gccaaatttg
     7981 cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc tttttttata
     8041 tttgcttaaa tctttttttg cgttacttat ggctgaccct ggcattttcc gctgtgcgtt
     8101 ttagcgttca tggtattaat cgctcagata tccacctttt cctcagattt ttttcattaa
     8161 atgcataaaa cttaatcacc aaatgttgct aaatattaag ctgattgttc tggtcatgtt
     8221 ggagtagcac aaatttctct gctgcaaata gctgtttgaa gtttttttaa tctttgtttg
     8281 tggattctca ccgttggtgt aaatcccggt tggctgtatt gacgttttca cattctgttg
     8341 acagattgta ggtcacgagg ggcattttat ggaggatccg cactgttaca ctgatgttaa
     8401 ttagtacggc atccccacct cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca
     8461 ggcagcgaca acccactgca aagggttaaa acaacaaaca tcacaattgg agcagaataa
     8521 tgcgtatttc cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg gtggctatga
     8581 ccgtcgcagc aagtgttcag gctaaaactc tggtttattg ctcagaagga tctccggaag
     8641 ggtttaaccc gcagctgttt acctccggca ccacctatga cgcctcttcc gtaccgcttt
     8701 ataaccgcct ggttgaattt aaaatcggca ccactgaagt gatcccgggc ctcgctgaaa
     8761 agtgggaagt cagcgaagac ggtaaaacct ataccttcca tctgcgtaaa ggtgtgaagt
     8821 ggcatgacaa taaagaattc aaaccgacgc gcgaactgaa cgccgatgac gtggtgttct
     8881 cgttcgatcg tcagaaaaac gcgcaaaacc cgtaccataa agtttctggc ggtagctacg
     8941 aatacttcga aggcatgggc ttgccggagc tgatcagcga agtgaaaaag gtggacgaca
     9001 acacagttca gttcgtgctg actcgcccgg aagcgccgtt cctcgctgat ctggcaatgg
     9061 acttcgcctc tattctgtca aaagaatatg ctgatgcgat gatgaaagcc ggtacaccgg
     9121 aaaaactgga cctcaaccca atcggaaccg gtccattcca gttacagcag taccaaaaag
     9181 attcccgtat ccgctacaaa gcgtttgatg gctactgggg caccaaaccg cagatcgata
     9241 cgctggtctt ctctattacc cctgatgctt ccgtgcgtta cgcgaaattg cagaagaatg
     9301 aatgccaggt gatgccgtac ccgaacccgg cagatatcgc tcgcatgaag caggataaat
     9361 ccatcaacct gatggaaatg ccggggctga acgtcggcta cctctcgtat aacgtgcaga
     9421 aaaaaccgct ggatgacgtg aaagttcgcc aggcactgac ctacgcggtg aacaaagacg
     9481 cgatcatcaa agcggtttat cagggcgcgg gtgtatcagc gaaaaacctg atcccgccaa
     9541 ccatgtgggg ctataacgac gacgttcagg attacaccta cgatcctgaa aaagcgaaag
     9601 ccttgctgaa agaagcgggt ctggaaaaag gtttctccat cgacctgtgg gcgatgccgg
     9661 tacaacgtcc gtataacccg aacgctcgcc gcatggcgga gatgattcag gcagactggg
     9721 cgaaagtggg cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg
     9781 cgaaagatgg cgaacatcag acggtaatga tgggctggac tggcgataac ggggacccgg
     9841 ataacttctt cgccaccctg ttcagctgcg ccgcctctga acaaggttct aactactcaa
     9901 aatggtgcta caaaccgttt gaagatctga ttcaaccggc gcgtgctacc gacgaccata
     9961 acaaacgcgt tgaactgtac aagcaagcgc aggtggtgat gcacgatcag gctccggcac
    10021 tgatcatcgc tcactccacc gtgtttgaac cggtacgcaa agaagttaaa ggctatgtgg
    10081 ttgatccatt aggcaaacat cacttcgaaa acgtctctat cgaataatta aaagccatac
    10141 ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca ttctctgtcc
    10201 gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc tgcgggtcac
    10261 tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc gtcatcccca
    10321 cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg ggcgatccgg
    10381 tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgcgcag ctgctggctg
    10441 aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg ggcgtaatgc
    10501 acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag ttcgtgccgc
    10561 gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg gcagtcggta
    10621 ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac acagcggttg
    10681 gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg ctgatcatgc
    10741 tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc
    10801 tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc tggggtgaag
    10861 acggcaactt tatcgatgcc gtcgcccata tgatcttgcc tgccattgtg ctgggtacta
    10921 ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg ctgggcgagg
    10981 attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt atcgtccatg
    11041 cgctgcgtaa cgcgatgctg ccggtggtga ccgttatcgg cctgcaggtg ggaacattgc
    11101 tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctggga cgctggttga
    11161 ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg ctggtggcga
    11221 cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg aacccgcgta
    11281 ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa gtgattagcg
    11341 caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc aacaaaggcg
    11401 cggtcgtcgg gctggtttac gtcgtcatcg tgctgttcat cgcgatcttt gccaactgga
    11461 ttgcacccta taacccggcg gaacagttcc gcgatgcact gctcgccccg ccagcctggc
    11521 aggaaggcgg cagcatggcg cacttgctgg gcaccgatga cgtaggccgt gatgtgctgt
    11581 cgcgcctgat gtacggtgcg cgcctgtcgc tgctggttgg ctgtctggta gttgtgttat
    11641 cgctgattat gggcgttatt ctcggcctga tcgccggtta ctttggcggc ctggtcgata
    11701 acatcattat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg ctggcgctgg
    11761 tgctggtggc aattttcggc ccgtcgattg gtaacgccgc gctggcactg accttcgttg
    11821 ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaagtt aaccgcgatt
    11881 acgtcaccgc gtctcgcgtg gcgggtgccg gggcgatgcg tcagatgttt attaacatct
    11941 tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct aacgccattc
    12001 tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg cctgagtggg
    12061 gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc
    12121 cgggtctggc gatcctgctg acggtgctgg catttaacct gatgggtgac ggtctgcgtg
    12181 acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt aaatgtagat
    12241 aaattatcgg tgcatttcgg cgacgaaagc gcgccgttcc gcgccgtaga ccgcatcagc
    12301 tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc cggtaagtcg
    12361 gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat ggcagaaaaa
    12421 ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg caacctggtg
    12481 ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc gtgctacacc
    12541 gtgggtttcc agattatgga agcgattaag gtgcatcagg gcggcaacaa aagtacccgc
    12601 cgtcagcgag cgattgacct gctgaatcag gtcggtattc ccgatccggc atcgcgtctg
    12661 gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat cgccatggcg
    12721 attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct ggacgtgacc
    12781 attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa catggcgctg
    12841 gtgttaatta cccatgacct ggcgctggtg gcggaagcgg cacataaaat catcgtgatg
    12901 tatgcaggcc aggtggtgga aaccggtgat gcgcacgcca tcttccatgc gccgcgtcac
    12961 ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg
    13021 gcgtcgttgc caggtgtcgt tcccggtaag tacgaccgcc cgaacggctg cctgcttaac
    13081 ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct gaatatgctc
    13141 gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag gccgacacta
    13201 tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac ctgaaaaaac
    13261 attatccggt gaagaaaggc atgttcgcgc cggaacgtct ggttaaagcg ctggatggca
    13321 tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta
    13381 aatcgaccct cggtcggttg ctgacgatga ttgaaatgcc caccggtggc gagctgtatt
    13441 accaggggca ggatctgctt aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa
    13501 tccagatcgt cttccagaac ccttacggtt cgctgaatcc gcgtaaaaaa gttgggcaaa
    13561 ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt cgggaaaaag
    13621 ccctgtcgat gatggcgaaa gtcggcctga aaaccgagca ctatgaccgc tatccgcata
    13681 tgttctccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg ctcgacccgg
    13741 atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt gcgcaagtgc
    13801 tgaatctgat gatggattta cagcaggaac tggggctttc ttatgtcttt atctcccacg
    13861 acctgtcggt ggtggagcac attgccgatg aagtgatggt gatgtacctg ggccgctgcg
    13921 tggagaaggg aacgaaagac caaatcttca ataacccgcg ccatccgtac actcaggcgc
    13981 tactctccgc gacgccgcgc ctgaacccgg acgatcgccg cgaacgcatc aaactcaccg
    14041 gtgaactgcc aagcccgctg aatccaccgc cgggttgcgc cttcaacgcc cgctgtcgtc
    14101 ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacggc ggtcaactgg
    14161 tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca aaaccggggc
    14221 catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc aaaatgccgt
    14281 aaagcagaat tagccaggct ttaaatccgc gaagtttttc cagttgtgcg actttataga
    14341 tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc aactgaaaga
    14401 acaccagcac cgatacacga aacgaaaccc aaatcgtcaa tgtaatgacg ataaaagcgc
    14461 agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt cggcttaaca
    14521 gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca aagaatgcgg
    14581 tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt ccagggatca
    14641 cctgcgcggc cagcgccagt gccgagatat tttgttcaaa ggcagaaacg gcttcttcgt
    14701 ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat
    14761 aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatcc gcttcccgtt
    14821 tacgataggc aatattcatt gggttaagta cctgaataaa tactgcagaa aagaagcaaa
    14881 atggaatggt aagcaagaca tcgcggaaaa agacggaggc ttgcgggaag gcggttatat
    14941 tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc cctactttga
    15001 ccaccaccat tggcccggaa atcttaaaca gtaatcgttc accaccagac gcaatcgcca
    15061 ccagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg gcatcggtta
    15121 aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag tagataaaaa
    15181 tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag ttcttcccca
    15241 ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa agggttttta
    15301 aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg gtaataaata
    15361 cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca ccaatcgcca
    15421 tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt
    15481 gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa
    15541 atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa
    15601 aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac aaaattgcgc
    15661 atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca aataaagaaa
    15721 ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt aaaaagcaat
    15781 cgtaaaatca gcggcaagcc gaaagggttc cggtgaggcg caatgttgcg ggggctttat
    15841 ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt atgtacctga
    15901 caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg aaacgttgtc
    15961 attacaacac aggcggctat atgacgctcg cagagctggg catggccttc tggcatgatt
    16021 tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg ctgaacaagc
    16081 ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc ccggaccagg
    16141 ccgcaggagt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt gcaagcggat
    16201 aatattctgc ggaatgcgca gcacagagtg aagatgagac ggattccgga acagaaaaac
    16261 agtcgtagaa tcagcgtcaa gccggaagga ttccggtgag gcgcaatgtt gcgggggctt
    16321 aatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc
    16381 tgacaacacc acgggggcca ttcttgcatc caaacaatac aagaatagcc gcgaaacgtt
    16441 gtcattacaa tacaggcggc tatatgacgc tcgcagaact gggcatggcc ttctggcatg
    16501 atttagcggc tccggtcatt gctggtattc ttgccagtat gatcgtgaac tggctgaaca
    16561 agcggaagta acgtgccatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc
    16621 aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg gttgcaagcg
    16681 gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc ggaacagaaa
    16741 aacagtcgta gaatcagcgg caagccggaa gggttccggt gaggcgcaat gttgcggggg
    16801 ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgc
    16861 acctgacaac atcacggggg ccattcttga acctgaacaa ctcaagaata gccgcgaaac
    16921 gttgtcatta caacacaggc ggctatatga cgctcgcaga actgggcatg gccttctggc
    16981 atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg aactggctga
    17041 acaagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg
    17101 accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcag
    17161 gcggataata ttctgcggaa tgcgcagcac agagtgagta agtgatggat ttcggaacag
    17221 aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc aatgttgcgg
    17281 gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta
    17341 tgcacctgac aacataacgg gggctaatct tgaacccaaa caactcaaga atagccgcga
    17401 aacgttgtca ttacaacaca ggcggctata tgacgctcgc agagctgggc atggccttct
    17461 ggcatgattt agcggctccg gtcattgctg gcattcttgc cagtatgatc gtgaactggc
    17521 tgaataagcg gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc
    17581 cggatcaggc cgcagaggtt aaactctgcg gcctttttcg tgcttactgc gggtaaggca
    17641 cccagtcgcc gccgttcagg cgaacgtacg gtttatcctg gtattgaata actactgcat
    17701 ttgagttctc ggagaccggt gctgtttgtg gcaacccact ggtgagtttt ttccagtcaa
    17761 cattgtcttc ggtgaaaatc ttgccatcaa gaacgcgaac caccagatcg gagatagcca
    17821 ggaagctgct cggttggtca atgacaatcg gtgccccctg atgtggtgcc ttcatgccga
    17881 agaatttcac cccaacgggg acgtctgtga tagacgggct agggatatca cgtaggccag
    17941 atacctgcat tctgtcgccc ttaagcgcgc cgccgtgttc cggcaccacg accaccatca
    18001 ctttacgacc cgatttctcc agttcagtaa agaaggcgtc cagttcatca aagaatttct
    18061 gcgcccgcgc tttgtaatcc gctgttttgc tgacccccgg ataatggttg ccgtcatgca
    18121 gtggaagcgt gttgtagaac gtggcactac ggctgttttt atctttttcg gtaacgtcca
    18181 gccagcggtt aagcacggcg gtatcgtcat aaaccggcga accatcaaag cccagcaaaa
    18241 taaccggcag atttgtttga tccatcaatt cagtctgcat gccgccattt tcgcgaactt
    18301 ctttcaaaaa accgccgaac tggccgttat gccccatcat caggtgctgg gtaaagccca
    18361 gtttcgaaag gttatcaaac agatagcagt cgttatttgc cggttgatac agattagtgt
    18421 gcgaagtctg cccgcagctg gcgcgcagta aacggatcgc cgccgggcca ctgtaggagg
    18481 tggcggagtt aaagttcttg aactcaatat cgaaatgcga ccacagtgga tgcgacatca
    18541 acccggcggc ttctatatcc gaccaggaaa gcgaacagat gttaatcacc agtagttcaa
    18601 atggctgagc atcagcgggc agcgaagacg ggaaggtcga tttacgtttc gcctccgcgt
    18661 tatagaaatt attcagccag gcgttaaggt tcgccgttgt tggcggtgca gtttgtgcgg
    18721 gcatatcacc cactaccggt gcgccacccg tcgccgcaac ggttgccgct gcgttaccac
    18781 ccgtcgttgt tacagtggtc gtcggttgtc cggctggcca caaggagaaa cttggtcccg
    18841 ccagggtaag tacgttcagc catagcagta tggcaaccac aaaaacggta atgcgaatcc
    18901 attgtgacag gaataaccag gccactaata aaacaaaaat ggccccaatc atctgccagt
    18961 taataaagcg tgtgacaagg tcgattaaat aatcggtact gaaccccgcc acctgcgaac
    19021 cctggctcat tatgctttcc gggccaggca accaggtgtc atgccagaac aaagcaaagc
    19081 cgatcggcag ggcaatccag tggcgcaagc gatgcaggct gtagcgcgga atgggcatca
    19141 gcagaaacgc ggcaaacacc aaattgagga gcggatggaa gttaagatat cccgcccaca
    19201 acaggccgaa cttaaccaga aaatagaagt tccagccgga aaggccgcgc cagtattgcc
    19261 agagggaaga aggcatggcg gtattttgcg taaattgagt cattttttgg ttgccctggc
    19321 tttttccgtg cggcgtaacg tcccggccgg tttaacataa cgaggtttag caaagaacaa
    19381 acgtaaggtg tcgcgaatgc gtcgcaaaga gtgccgcgcc agatagccca gcgggaaaaa
    19441 tatcagtgcg caaacgacaa taatttcaat gatatcgctg atggtcatca tgatgagcgc
    19501 tccacagcat catctaacag tcgcatgggt tctggtattc gtcgccagtg gcgaccatcg
    19561 tgctcggcat tgatgaccgg tttagaactt tgcgttaaag gcagcggcat gccccattgt
    19621 tctggggcaa gcaagcgcat ctgcaccagc tcggcactga tttgatcatc ttcaaaccag
    19681 accatacggt ttgagaaaat gtcgccagta ggcaatggga aaatatgatt caacgcggta
    19741 tccagatcgt taatccgaca gaatgagaga aacagcacca gccgattacc gccaatggtc
    19801 atgatatcgc cggtacggtt agggcgacac agcgtcaggg cttgttcaac gcggataccc
    19861 ggtaccggac gtagggcaac cagaacgcct ttaccgtggg caggtaatag agggttattc
    19921 atcatgttgt tgacggcatt acagaacaca tcccacttct ggaaaccacg cagtttgagc
    19981 ggctgggtca ttgacagcaa ggtagtgata tcttccggca catagcgact aaacttctgc
    20041 ccttgcacgc tttcgatcat cgtcagacaa cgggagagtg gcgcattcca cggaataacc
    20101 atatttgcac cgcaggccaa taacaaacgt tcatcggtgg cgcgcaggct agcggtattt
    20161 tcccgcacga ggattttcat cgcactaccg cgctggcgac gcagggtatg aatgctgcgg
    20221 gccagtggct cgatttgcgc attttgctgt aaagaaaaga ccaccgtcgc cgcctgagcg
    20281 gtacgggctt cattgaacag gacttcgtta ttgttgaaca gttgccagtg ttccgatagc
    20341 ggcggcgcac cttccagtac agcaacatta ctcagaatgc gtttttcgtc gctgcgtggt
    20401 tggatctccg cctcttcgct ttgaactaat gtccagatac cattttgttg ctgaacgcta
    20461 agctgctgac gggcgctgac ccctttttcg ttgcaccaga aggcaatatc cagcaaatgt
    20521 tggtcaccct gaaaacgcaa actggcaaga ccaaaaagtg aacggtattc ctcaagcaac
    20581 aatgaaaatt gtttatcgtt attatttccg ggattaatta ccaaaagcga acaatggttt
    20641 aacctgctcc atttattcat tttatccaac catgagcgaa gccgctcggc aggaatgttt
    20701 tgccatgcgt tatttgcgca aacaagaata aaaaggtaat tatggggatc aatagaacac
    20761 tgcaaatcac gggtcaaata gtatagacct ttttcatgat ttagcattga aaataatttt
    20821 attttttccg gcccttgaga atcatctaat tgaaagattt tcgccggatc gctgtccatg
    20881 ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt cgccagactg
    20941 atagcatctt catggcgatc gacgttaaac caccagacgc cgcctgctgg catatgtcgc
    21001 agctcatccc ataatgatga gataccgata gagaatacag ggtccacaat gtccctcatg
    21061 tcaccatttt gtctgattat cagtttacta gcgaaagcta agaaataaac ctaccattga
    21121 aattaaaact tatcagattt agcatgtgaa tcaatttaga aggtcacaag acgtgtgatt
    21181 ttagtctttt cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa gtgagcaaat
    21241 tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt gtctcatgaa
    21301 cggtacggtt atttcatagg gatcaagcaa aatgaataac aatgaaccag atactctgcc
    21361 tgatcccgcg ataggctata tcttccagaa tgatattgtg gcgttaaagc aggcattttc
    21421 actgcctgat attgattatg ccgatatttc ccaacgcgaa cagttggccg cggcattaaa
    21481 acgctggccg ttgctggcag agtttgcgca acaaaagtag gggattggtg aatggccgta
    21541 ctgggattgc agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc cgcattagcc
    21601 tggtcattac aaatgttggg agaaaatgtt ctggtggtcg atgcctgccc ggacaacttg
    21661 ttgcgcctgt catttaatgt tgattttacc caccgtcagg gctgggccag agcgatgctg
    21721 gatggccagg actggcgtga cgctgggttg cgctacacct cgcagctcga tttgctgcct
    21781 tttggtcagt tatccattga agaacaagaa aatccacagc actggcaaac ccggctgagc
    21841 gatatttgct ccggcttaca gcaactaaaa gccagcgggc gttaccagtg gattttaatc
    21901 gacttaccgc gtgatgcctc gcagataacc caccagctgc tgagtttgtg cgatcactcg
    21961 ctggcaatcg tcaatgtgga tgccaactgc catatccgac tgcatcagca agcgctgccg
    22021 gatggcgcac atattttgat taataacttc cgtattggca gtcaggttca ggacgatatt
    22081 taccagcttt ggttgcaaag ccagcgccga ttactgccga tgctcattca tcgtgatgaa
    22141 gcgatggctg aatgcctggc ggctaagcaa ccagtaggtg aatatcgcag tgatgcgctg
    22201 gcggctgaag agatactgac gctggcgaac tggtgcctgt tgaactactc cgggctgaaa
    22261 acgccagtcg ggagtgcatc atgagtatcc tgacccggtg gttgcttatc ccgccggtca
    22321 acgcgcggct tatcgggcgt tatcgcgatt atcgtcgtca cggtgcgtcg gctttcagcg
    22381 cgacgctcgg ctgtttctgg atgatcctgg cctggatttt tattccgctg gagcacccgc
    22441 gctggcagcg tattcgcgca gaacataaaa acctgtatcc gcatatcaac gcctcgcgtc
    22501 cgcgtccgct ggacccggtc cgttatctca ttcaaacatg ctggttactg atcggtacat
    22561 cgcgcaaaga aacgccgaaa ccgcgcaggc gggcattttc aggtctgcag aatattcgtg
    22621 gacgttacca tcaatggatg aacgagctgc ctgagcgcgt tagccataaa acacagcatc
    22681 ttgatgagaa aaaagagctc ggtcatttga gtgccggggc gcggcggttg atcctcggta
    22741 tcatcgtcac cttctcgctg attctggcgt taatctgcgt tactcaaccg tttaacccgc
    22801 tggcgcagtt tatcttcctg atgctgctgt ggggtgtagc gctgatcgta cggcggatgc
    22861 cggggcgctt ctcggcgcta atgttgattg tgctgtcgct gaccgtttct tgccgttata
    22921 tctggtggcg ttacacctct acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc
    22981 ttattctgct cttcgctgaa acgtacgcgt ggattgtgct ggtgctcggc tacttccagg
    23041 tagtatggcc gctgaatcgt cagccggtgc cattgccgaa ggatatgtcg ctgtggccgt
    23101 cggtggatat ctttgtcccg acttacaacg aagatctcaa tgtggtgaaa aataccattt
    23161 acgcctcgct gggtatcgac tggccgaaag acaaactgaa catctggatc ctcgatgacg
    23221 gcggcaggga agagtttcgc cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca
    23281 ccactcatga acatgcgaaa gcgggcaaca tcaacaatgc gctgaaatat gccaaaggcg
    23341 agttcgtgtc gattttcgac tgcgaccacg taccaacgcg atcgttcctg caaatgacca
    23401 tgggctggtt cctgaaagag aaacagctgg cgatgatgca gattccacac cacttcttct
    23461 cgccggaccc gtttgaacgc aacctggggc gtttccgtaa aacaccgaac gaaggcacgc
    23521 tgttctatgg tctggtgcag gatggcaacg atatgtggga cgccactttc ttctgcggtt
    23581 cctgtgcagt aattcgtcgc aaaccattgg atgaaattgg cggtattgct gtcgaaacgg
    23641 taaccgaaga tgcgcacacg tctctgcgcc tgcaccgtcg tggctacacc tctgcatata
    23701 tgcgtattcc gcaggcggcg gggctggcga ccgaaagtct gtcggcgcat atcggtcagc
    23761 gtattcgctg ggcacgcggg atggtgcaaa tcttccgtct cgataacccg cttaccggta
    23821 aagggctgaa attcgcccag cggctgtgct acgtcaacgc catgttccac ttcttgtcgg
    23881 gcattccacg actgatcttc ctgactgcgc cgctggcgtt cctgctgctt catgcctaca
    23941 tcatctatgc gccagcgttg atgatcgccc tgttcgtgct gccgcatatg atccatgcca
    24001 gcctgaccaa ctcgaagatc cagggcaaat atcgccactc tttctggagt gaaatctacg
    24061 aaacggtgct ggcgtggtat atcgcaccac cgacgctggt ggcgctgatt aacccgcaca
    24121 aaggcaaatt taacgtcacc gccaaaggtg gactggtgga agaagagtac gtcgactggg
    24181 tgatctcgcg gccctacatc ttccttgttc tactcaacct ggtgggcgtt gcggtaggca
    24241 tctggcgcta cttctatggc ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt
    24301 gggtgttcta caacctgatt gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac
    24361 aggtacgccg atcgcaccgc gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg
    24421 gtcacctctt ctcgtgtacc gttcaggatt tctccgacgg tggtttgggg atcaagatca
    24481 acggtcaggc gcagattctg gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc
    24541 aggaatacgt cttcccgacc caggtggcgc gcgtgatggg taatgaagtt gggctgaaat
    24601 taatgccgct caccacccag caacatatcg attttgtgca gtgtacgttt gcccgtgcgg
    24661 atacatgggc gctctggcag gacagctacc cggaagataa gccgctggaa agtctgctgg
    24721 atattctgaa gctcggcttc cgtggctacc gccatctggc ggagtttgcg ccttcttcgg
    24781 tgaagggcat attccgtgtg ctgacttctc tggtttcctg ggttgtatcg tttattccgc
    24841 gccgcccgga gcggagcgaa acggcacaac catcggatca ggctttggct caacaatgat
    24901 gataacgcga tgaaaagaaa actattctgg atttgtgcag tggctatggg gatgagtgcg
    24961 ttcccctctt tcatgacgca ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca
    25021 gctgtagccg cccagacgga acaaaatccg caggtggggc aagtgatgct gggcgtgcag
    25081 ggcgctgatg cgccagtcgt ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt
    25141 gcgcaaattg cgccgccgcc gggcagcatg gtgctacgtg gcattaaccc gaacggcagc
    25201 attgagtttg gtatgcgcag cgatgaagtg gtgacgaagg cgatgctcaa cctcgaatac
    25261 acgccatcgc catcgttact gcctgtccag tcgcagttaa aggtttatct caatgatgag
    25321 ctgatgggcg tgctgccagt gaccaaagaa cagttgggta aaaaaacgct ggcgcaaatg
    25381 cccattaacc cactgtttat taccgacttc aaccgtgtgc ggctggagtt tgtcggccat
    25441 tatcaggacg tgtgcgaaaa cccggccagc accacgcttt ggctggatgt tgggcggagc
    25501 agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc acacttcccg
    25561 gtgccattct ttgatccgcg tgataaccgc accaacacct tgccgatggt ctttgcgggt
    25621 gcgccggatg ttgggctgca acaagcctct gccattgtcg cctcgtggtt tggttcgcgt
    25681 tctggctggc gtgggcagaa cttcccggta ctctataacc aactgccgga tcgcaatgcc
    25741 attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa
    25801 gcccctgtga ttgagatgat taaccatccg cagaatcctt acgtcaaact gctggtggtg
    25861 tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca gggtaacatt
    25921 ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg
    25981 tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga actgaaaacc
    26041 tatgaagaac agttacaatc cagcggtctt gagccagcag cgattaacgt ttcgctaaac
    26101 ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat taattaccgc
    26161 tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa taaccagttc
    26221 ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gcctgctgct gcggattccg
    26281 gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct gaaactgggc
    26341 gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccggg cggttcggtg
    26401 gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga cgactccacc
    26461 atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc ctttgctaac
    26521 gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt gatgccgaaa
    26581 gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat cggcgcacag
    26641 acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat tcagggcaaa
    26701 gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga taagcagatc
    26761 gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca gaccccgttc
    26821 cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccggtcaac gctgacctct
    26881 tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca gcgcagcgtg
    26941 attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc ggtgaacgat
    27001 agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc cggtatcaac
    27061 agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga gcgcttgtgg
    27121 tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cggctatcag tgtgatattg
    27181 ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct taacccggat
    27241 aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct gctgttggct
    27301 gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa aaaggattac
    27361 atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac cacctccgaa
    27421 gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgtagc tttcgataat
    27481 attctcgact ggacgcagaa caatctcgct cagggctctt taaaagaacg tttgcccgcc
    27541 tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc
    27601 gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag
    27661 cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga agtggtgacg
    27721 gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga ggataacagc
    27781 tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac ccgctttggc
    27841 gcgccgtgga ccacgctgcg cgaaaccaat caacgtttat tgctggaaac cgccccgaaa
    27901 ggtttttcgc cagactgggt gcgctatgag aaagacaaag gctggcagct aaaagccgaa
    27961 aaaacattga tcagcagcta cgacgctatc cgcgtttaca tgtgggtagg catgatgcct
    28021 gacagcgatc cgcaaaaagc gcggatgctc aaccggttta aaccgatggc gacattcact
    28081 gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa
    28141 ggaccggtcg gtttttctgc cgccatgctg ccctttttac aaaaccgcga tgcgcaggcc
    28201 gttcagcgcc agcgcgtggc cgataacttt cccggcagcg atgcctatta caactatgtg
    28261 ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag
    28321 ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc acgctttccc
    28381 tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa cagttgctgg
    28441 agcaagttcg gttaggcgaa gcgacccatc gtgaagatct ggtgcaacag tcgttatatc
    28501 ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc cgttctttgt
    28561 tacgtcaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg cagttagcgc
    28621 cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg ccggatggtc
    28681 gtcaggcact gcaacaggca cgattgcagg cgacgaccgg tcatgcagaa gaagctgtgg
    28741 cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct gtcgagtact
    28801 ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag ttaaaacgca
    28861 tcaatgcgga tgcaccgggc aatacgggcc tgcaaaacaa tctggcgcta ttgctgttta
    28921 gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcaaaa tcgaacgccg
    28981 ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc gtcagtgatg
    29041 ccagtgtgtc ggcgctgaaa aaatatctct cgatctttag tgatggcgat agcgtggcgg
    29101 ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct ttccgcgctc
    29161 gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt cccgaactac
    29221 aacaggcggt gcgggcgaac ccgaaagaca gtgaagctct gggggcgctg ggccaggcgt
    29281 attctcagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc ctcgcactgg
    29341 acccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac cgctactggc
    29401 tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg gcagaacgcc
    29461 tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg gggctgggcg
    29521 atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag cagaccttgc
    29581 gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac cgccagcaat
    29641 cgccagaaaa agctgaagcg tttatcgcct cgctctctgc cagtcagcgg cgtagcattg
    29701 atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag gcactggaaa
    29761 accagggcaa atgggcgcag gcggcagcac ttcagcggca acgactggcg ctggaccccg
    29821 gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc
    29881 aggccgatac gttaatgcgc aatctggcgc agcagaagcc gaacgacccg gagcaggttt
    29941 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
    30001 acagcctgcc gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa
    30061 gcgatcaggt gctggaaact gctaatcgcc tgcgagaaag cggcaaagaa gctgaagcgg
    30121 aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
    30181 cgcagcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
    30241 agccaactaa cgccgatgcc attctggggc tgacggaagt ggatattgct gccggtgaca
    30301 cagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca
    30361 cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg gcgcagcaga
    30421 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
    30481 tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa
    30541 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
    30601 cattcacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca
    30661 gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact
    30721 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
    30781 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
    30841 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
    30901 tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
    30961 ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg
    31021 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
    31081 cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    31141 gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct
    31201 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    31261 aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta
    31321 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    31381 atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt
    31441 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    31501 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    31561 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    31621 gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg
    31681 gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg
    31741 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    31801 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    31861 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    31921 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    31981 ccgttttgct gttccatctg gtccaacaga atcgctataa cacggctacg caactggaaa
    32041 gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg
    32101 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    32161 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc ccggtgccgg
    32221 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg
    32281 aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat tccttccgta
    32341 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgc
    32401 ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca
    32461 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    32521 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    32581 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    32641 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    32701 aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga
    32761 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    32821 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    32881 tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcactatc atgagcgagc
    32941 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct
    33001 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    33061 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    33121 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt
    33181 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    33241 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    33301 gctgtgggct catggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    33361 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac
    33421 tgatgcaccc gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    33481 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    33541 tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca
    33601 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga
    33661 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    33721 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    33781 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc
    33841 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    33901 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    33961 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    34021 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    34081 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    34141 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    34201 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    34261 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    34321 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    34381 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    34441 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    34501 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    34561 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    34621 catcttcggc atcatcaata tgatcatgcg tctggcgcct attggtgcgt tcggggcaat
    34681 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    34741 ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    34801 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    34861 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    34921 ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    34981 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    35041 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    35101 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    35161 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    35221 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    35281 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    35341 cgaattatcc tcttaatctc atcacttatg cccgcactcc cttccccgag tgcgggtgtt
    35401 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    35461 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    35521 gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc
    35581 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    35641 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    35701 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    35761 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    35821 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    35881 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    35941 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    36001 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    36061 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    36121 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    36181 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    36241 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat aaacaaaacg tttggcgaac
    36301 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    36361 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    36421 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    36481 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    36541 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    36601 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    36661 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    36721 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    36781 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    36841 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    36901 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    36961 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    37021 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    37081 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggagtaacac
    37141 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    37201 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    37261 cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    37321 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    37381 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    37441 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    37501 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    37561 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    37621 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    37681 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    37741 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    37801 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    37861 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    37921 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    37981 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    38041 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    38101 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    38161 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    38221 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    38281 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    38341 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    38401 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    38461 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    38521 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    38581 gattgatttg ctggcgcaaa aagccgactt ctttatagag cacggcctgc tggcatcggt
    38641 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    38701 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    38761 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    38821 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    38881 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    38941 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    39001 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    39061 gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgcctggct
    39121 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    39181 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    39241 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    39301 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    39361 agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag acatcattct
    39421 tggcaatcca ccggatattc ccgaagtcac gatggtgcat ttgccacgcg tagaggcaac
    39481 gctggccccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    39541 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatctcgc
    39601 caacgatgat aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    39661 tattcttttc gatcaagggc ggatcgctat tgatgacaaa gtaagcaaag cggatctgga
    39721 gatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    39781 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    39841 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    39901 agggacgccg tttccggtac aggctgattt ccgctctggt aacacccgtg ttgcttttga
    39961 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    40021 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    40081 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    40141 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    40201 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    40261 cctgggaccg ttaattggcg ttgactccgg gaaaggggca gaaaagtcga aacggtctga
    40321 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    40381 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    40441 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    40501 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    40561 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    40621 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    40681 tgacgcggaa ctacgcggta gcggtaactc ggtggcagca cttttaggca acagtaacgg
    40741 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    40801 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    40861 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    40921 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct
    40981 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    41041 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccgggc cgttgattgc
    41101 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    41161 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    41221 gaagtgatga ggttggattg tgtttgctgg tgagttcggg agcagcgtgt tcccctcacc
    41281 ctaaccctct ccccaaaggg gcgaggggac tgttttgtgc gtgttgaaac aacgagtacc
    41341 gcttttccgt tcactttctg ggggagggct gttatgttcg cgatataaca tctcgctccg
    41401 cttttctccc tctccctttc agggagaggg ctggggtgag ggtggctgcc cacacgatac
    41461 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    41521 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    41581 gatatatggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga acgatgctcc
    41641 ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg gcccgaaggt
    41701 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    41761 accaagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgataattac
    41821 catactttta cggcgaccaa aggcatctgc cagtaatccg gcgactggca ccatcacgcc
    41881 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    41941 aactggcgcg gcggcggtac taaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    42001 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    42061 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    42121 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    42181 tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg aaagcagcaa
    42241 gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga aggagccata
    42301 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc cgccccattc
    42361 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg gggcgaaaat
    42421 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    42481 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    42541 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    42601 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    42661 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    42721 attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg ctgttgcttg
    42781 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    42841 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    42901 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    42961 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggaa
    43021 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    43081 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    43141 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    43201 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    43261 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    43321 aatgctgcgc cagaggggga tttcatcagc gatggcaagg tgtaggtcat cacgatttta
    43381 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    43441 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    43501 atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    43561 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgacag cgtcacaatc
    43621 agcgcaatta acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    43681 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    43741 aggttaccca tccagttgat gccagaataa agcgccaccg ccaggccgac aagccctaca
    43801 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagcgtcgga
    43861 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    43921 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga taaaaatgag
    43981 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    44041 cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    44101 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    44161 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    44221 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    44281 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    44341 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    44401 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    44461 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    44521 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    44581 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    44641 agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg ggcgataacg
    44701 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    44761 accggacggt catggctact cagttccagc tcaattcccg gatgcagata gagaaattcc
    44821 gacaggcgcg gcagtaacag gcttttcgcg attcctggcg ggatgtcgat gcgtaattta
    44881 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    44941 acatcttttg ctcgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgtg
    45001 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    45061 cttccttttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    45121 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    45181 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    45241 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    45301 ctgagaaagg tgaatccccc taaacagagc gtatttttca gcgattccct ttaaagtgaa
    45361 tagaggacgt gttggctatt tgtgcttatt tttgtctcat tgccgtttgt tatttttgct
    45421 tgtggtcgcg gagtttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    45481 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    45541 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    45601 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    45661 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    45721 tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc agtcaggcag
    45781 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    45841 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    45901 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt attcatttta
    45961 atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg gcggtaaata
    46021 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    46081 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    46141 gcgaatcaaa taaggagatc agcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    46201 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    46261 taaatatctc ctcctgaaga ggataatttt acagccagca ggaagttagc ggctggctgt
    46321 aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg taccacaccg ttagtccagc
    46381 caaacccatc ctgcaacgga tactcgccac cgccgccttc gcggggaaca ccatcggcaa
    46441 tatggtattt ctcgatcatt ttgtgctgtt ccagatagaa ctgattcacc gttttcagcc
    46501 agctgcgcgc gatttcatca cccagaaggt catcgccata cattttaaat ccctgaattg
    46561 ccatccattg taacggtgcc cagccattgg gtttatccca ctgttcaccg gtttcgtact
    46621 cgcttgccag aatcccgcca ggtgtcagta accggctgcg tacggcgttt gccagacgat
    46681 cggcctgttc atggttcgcc atgccgacat agagcggcac aatggcggca gcggaaaaca
    46741 gcgccagttg ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca tcatcccaga
    46801 ggtaacggtt taccgcatcg cgacgggcac tggccttctg gcggaacagc gcttctgtct
    46861 ctttctcgcc tttcagcgcc gagatgttgg cgatggcgct ctccagttta aacaggaagg
    46921 cattcagatc gatggggatg aactgggtgg tacgaatgct cgccagacga ccagtatcac
    46981 gcagccaacg ggaagagtaa tcccaacctg aggccgctcc cgcgcgtaaa tcgcggtaca
    47041 cctcgttggg cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc caggattcgt
    47101 cacgcggcgt gtcgcgatcg tcccaataac ggttgagcag cgatccgtcc ggcatccgca
    47161 caacatggcg ataggcctga ttagggatca acgattctgc accgtccatc cagaaggcat
    47221 attccatttt aaggtggtcg agatagcggc gcgcaccgcg tacaccatct tcttcaaaca
    47281 actccaccat cagcgcaaaa accggtggtt gcgatcggct caaataatag gtgcggttgc
    47341 cgttggggat gtgaccatag ttttcgatca tccaggcgaa gttatcggcc atgcatttca
    47401 gcaaatcttc ccgaccactt tccgccagcc ccagcatggt gaaataggaa tcccaatagt
    47461 aggtttcgct aaaacggccg cccgggacaa tatatgactg cggtagcgcc agcagagaag
    47521 accacggaat gtgatcctgt ggttcgcggg ttagcaccgg ccacagctgg tcgatatgct
    47581 ctttcaggga attttgcggg tccgatacat actcgctgga gtagacctcc ggcagccaga
    47641 agtgattttc aacaaacttg cgcaagtcaa aatcacgatg acggcgcact ttacggtagc
    47701 ggattaaaat atccagcggg tccattttgg gtgcgcagtc gggaaaggtt ttactgtcgg
    47761 ggaaaatttt cgccgactga acatgctcga acagttcgag atagcgatcg gcaggcgtca
    47821 gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc cggttctgcc tcgatcatct
    47881 catccagttt taattcatac gggtccagct catagcagag atcgacttcg atcatcagtt
    47941 cgtctggatt agggttttga attttctgat tgagcataag gatccaacct ccgaaattcg
    48001 tcgctaatta tcgggctttt ctttaagggt agtcattcgc tccgcctggt gggcgacaaa
    48061 gcgtgcggtg gatcacgttt attaacctgt caaaaaatat aacgcactaa tatccatgga
    48121 atttataaaa atacggattt cgtgttgttt ttcattttca gaacattcaa tcctgctctc
    48181 gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat cttattgata
    48241 gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg ttttttatga
    48301 tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt cacaataact
    48361 caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat cggcctggct
    48421 ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga taataaacgc
    48481 agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt tttaggcttt
    48541 ctccgcgaaa aaggatgcga ctattgccac acaccttcgg cagaattacc cgcctattat
    48601 tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata taaatctttt
    48661 aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca aagcgatctg
    48721 aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta taccgcgcta
    48781 cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg gattgcaaaa
    48841 cagcgcgcag aatattacgc cagcaatgat actgctccgg agcatcgcaa tgaaccggtg
    48901 cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg ctttgcgctg
    48961 tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg ccatgcgttg
    49021 aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc agttgggccg
    49081 attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg ggatggtcgt
    49141 gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga aatggcgtcg
    49201 aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa agcgcagttc
    49261 ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat tgctgaattt
    49321 gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg agatgaaaat
    49381 gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa atgtgcaact
    49441 tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct gaaaaaagac
    49501 tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac taaagaagag
    49561 cgtgataaat tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac ggcaccgtac
    49621 ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct gcgctatcag
    49681 gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct gcacagtctg
    49741 aacggggtgt acacgccgta tatgcaggat aaacaataa
//
LOCUS       DAZGCS010000023        49291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGCS010000023 DAZGCS010000000
VERSION     DAZGCS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.023"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..281
                     /locus_tag="ACHK01_001415"
     CDS             <1..281
                     /locus_tag="ACHK01_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFO8096388.1"
                     /translation="KSNSGSTVITGRIANGQQIPLPTGFSASQCSWSVSNAENPQGWK
                     PNYFAGSVATYDANRIVKCGFYDEYNFHKGTFRADLTGKCSYVVACQN"
     gene            854..2008
                     /locus_tag="ACHK01_001416"
     CDS             854..2008
                     /locus_tag="ACHK01_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8096389.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2159..2983
                     /gene="traE"
                     /locus_tag="ACHK01_001417"
     CDS             2159..2983
                     /gene="traE"
                     /locus_tag="ACHK01_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO8096390.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3068..4270
                     /locus_tag="ACHK01_001418"
     CDS             3068..4270
                     /locus_tag="ACHK01_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO8096391.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4330..4914
                     /locus_tag="ACHK01_001419"
     CDS             4330..4914
                     /locus_tag="ACHK01_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFO8096392.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5309..5767
                     /gene="traH"
                     /locus_tag="ACHK01_001420"
     CDS             5309..5767
                     /gene="traH"
                     /locus_tag="ACHK01_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFO8096393.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            5764..6582
                     /gene="traI"
                     /locus_tag="ACHK01_001421"
     CDS             5764..6582
                     /gene="traI"
                     /locus_tag="ACHK01_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFO8096394.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            6579..7727
                     /gene="traJ"
                     /locus_tag="ACHK01_001422"
     CDS             6579..7727
                     /gene="traJ"
                     /locus_tag="ACHK01_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO8096395.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            7724..8014
                     /locus_tag="ACHK01_001423"
     CDS             7724..8014
                     /locus_tag="ACHK01_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO8096396.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8029..8580
                     /locus_tag="ACHK01_001424"
     CDS             8029..8580
                     /locus_tag="ACHK01_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFO8096397.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            8670..12437
                     /locus_tag="ACHK01_001425"
     CDS             8670..12437
                     /locus_tag="ACHK01_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO8096398.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPNPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PTPDSIVYAPERPDAPISLNLDEIIKSLKGEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     DGASNDDRSVLAALAVATNFYGGVIELTGSDAFKQKAMRLIIEHNINVRMKHADQRLE
                     LDTLRKEMAVGKDAVVTHQPTPELNRNTPEQPVVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            12455..12802
                     /locus_tag="ACHK01_001426"
     CDS             12455..12802
                     /locus_tag="ACHK01_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO8096399.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            12799..13491
                     /locus_tag="ACHK01_001427"
     CDS             12799..13491
                     /locus_tag="ACHK01_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO8096400.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            13502..14485
                     /gene="traN"
                     /locus_tag="ACHK01_001428"
     CDS             13502..14485
                     /gene="traN"
                     /locus_tag="ACHK01_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFO8096401.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            14488..15777
                     /gene="traO"
                     /locus_tag="ACHK01_001429"
     CDS             14488..15777
                     /gene="traO"
                     /locus_tag="ACHK01_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO8096402.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKVSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            15777..16481
                     /gene="traP"
                     /locus_tag="ACHK01_001430"
     CDS             15777..16481
                     /gene="traP"
                     /locus_tag="ACHK01_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFO8096403.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            16481..17008
                     /gene="traQ"
                     /locus_tag="ACHK01_001431"
     CDS             16481..17008
                     /gene="traQ"
                     /locus_tag="ACHK01_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO8096404.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17059..17463
                     /gene="traR"
                     /locus_tag="ACHK01_001432"
     CDS             17059..17463
                     /gene="traR"
                     /locus_tag="ACHK01_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFO8096405.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISSISIG"
     gene            17527..17715
                     /gene="traS"
                     /locus_tag="ACHK01_001433"
     CDS             17527..17715
                     /gene="traS"
                     /locus_tag="ACHK01_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFO8096406.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            17699..18499
                     /gene="traT"
                     /locus_tag="ACHK01_001434"
     CDS             17699..18499
                     /gene="traT"
                     /locus_tag="ACHK01_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFO8096407.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            18589..21633
                     /gene="traU"
                     /locus_tag="ACHK01_001435"
     CDS             18589..21633
                     /gene="traU"
                     /locus_tag="ACHK01_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFO8096408.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            21633..22247
                     /gene="traV"
                     /locus_tag="ACHK01_001436"
     CDS             21633..22247
                     /gene="traV"
                     /locus_tag="ACHK01_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFO8096409.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22214..23416
                     /gene="traW"
                     /locus_tag="ACHK01_001437"
     CDS             22214..23416
                     /gene="traW"
                     /locus_tag="ACHK01_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFO8096410.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            23445..24029
                     /gene="traX"
                     /locus_tag="ACHK01_001438"
     CDS             23445..24029
                     /gene="traX"
                     /locus_tag="ACHK01_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFO8096411.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24126..26288
                     /locus_tag="ACHK01_001439"
     CDS             24126..26288
                     /locus_tag="ACHK01_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO8096412.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTQTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTSVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDKGSDDKDGIKG"
     gene            26353..27015
                     /gene="excA"
                     /locus_tag="ACHK01_001440"
     CDS             26353..27015
                     /gene="excA"
                     /locus_tag="ACHK01_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO8096413.1"
                     /translation="MKDKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
     gene            complement(27087..27296)
                     /locus_tag="ACHK01_001441"
     CDS             complement(27087..27296)
                     /locus_tag="ACHK01_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO8096414.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            27688..27864
                     /locus_tag="ACHK01_001442"
     CDS             27688..27864
                     /locus_tag="ACHK01_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096415.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(27929..28024)
                     /gene="dqlB"
                     /locus_tag="ACHK01_001443"
     CDS             complement(27929..28024)
                     /gene="dqlB"
                     /locus_tag="ACHK01_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO8096416.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            28585..28776
                     /locus_tag="ACHK01_001444"
     CDS             28585..28776
                     /locus_tag="ACHK01_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096417.1"
                     /translation="MADALEKRSGSEENALAMLAMTAFTLMTRRGICEMQNVAPDGKG
                     IRLELIGFRGNETIPDTLH"
     gene            complement(28848..29000)
                     /locus_tag="ACHK01_001445"
     CDS             complement(28848..29000)
                     /locus_tag="ACHK01_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8096418.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            29072..30145
                     /locus_tag="ACHK01_001446"
     CDS             29072..30145
                     /locus_tag="ACHK01_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO8096419.1"
                     /translation="MQMQHLMVGYPKYYQTADYALRLSVMADIATMRMKALHFWDKHG
                     ISAASEAFGVSCRTLYWWRQLLNKEGPEGLIPHSKAPLVRRKKHWHPDVLKEIRRLRT
                     ELPNLGKEQIFVRLKPWCEQRYLACPSVSTIGRMIAAAHDKMRMIPVRLGSRGKALLV
                     KKRSAKPRRPKHYRPVKTGELIGMDAIELRMGELRRYVITMIDECSNYALALAVPSLN
                     SDIVNHFFSRAARLFPVGISQIITDNGKEFLGNFDKTLQEAAIKHLWTYPYTPKMNAI
                     CERFNRTLREQFIEFNEILLFEDLALFNQKLAEYLVLYNSKRPHKALALMTPVEYILK
                     ENKNCNMWWTHTLHCLTAVIGLL"
     gene            30393..31601
                     /gene="trbA"
                     /locus_tag="ACHK01_001447"
     CDS             30393..31601
                     /gene="trbA"
                     /locus_tag="ACHK01_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO8096420.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPYIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            31620..32690
                     /gene="trbB"
                     /locus_tag="ACHK01_001448"
     CDS             31620..32690
                     /gene="trbB"
                     /locus_tag="ACHK01_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFO8096421.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFLYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            32683..34974
                     /gene="trbC"
                     /locus_tag="ACHK01_001449"
     CDS             32683..34974
                     /gene="trbC"
                     /locus_tag="ACHK01_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO8096422.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCMREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(35011..37710)
                     /gene="nikB"
                     /locus_tag="ACHK01_001450"
     CDS             complement(35011..37710)
                     /gene="nikB"
                     /locus_tag="ACHK01_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFO8096423.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(37721..38053)
                     /gene="mobA"
                     /locus_tag="ACHK01_001451"
     CDS             complement(37721..38053)
                     /gene="mobA"
                     /locus_tag="ACHK01_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO8096424.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            38286..38621
                     /locus_tag="ACHK01_001452"
     CDS             38286..38621
                     /locus_tag="ACHK01_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO8096425.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(38707..39555)
                     /locus_tag="ACHK01_001453"
     CDS             complement(38707..39555)
                     /locus_tag="ACHK01_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8096426.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            39904..40101
                     /locus_tag="ACHK01_001454"
     CDS             39904..40101
                     /locus_tag="ACHK01_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096427.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            40135..40386
                     /locus_tag="ACHK01_001455"
     CDS             40135..40386
                     /locus_tag="ACHK01_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO8096428.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(40417..40614)
                     /locus_tag="ACHK01_001456"
     CDS             complement(40417..40614)
                     /locus_tag="ACHK01_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096429.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEQ"
     gene            complement(40637..41575)
                     /locus_tag="ACHK01_001457"
     CDS             complement(40637..41575)
                     /locus_tag="ACHK01_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8096430.1"
                     /translation="MSQKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(41640..41906)
                     /locus_tag="ACHK01_001458"
     CDS             complement(41640..41906)
                     /locus_tag="ACHK01_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096431.1"
                     /translation="MKPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(42000..42434)
                     /locus_tag="ACHK01_001459"
     CDS             complement(42000..42434)
                     /locus_tag="ACHK01_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO8096432.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(43163..43663)
                     /locus_tag="ACHK01_001460"
     CDS             complement(43163..43663)
                     /locus_tag="ACHK01_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO8096433.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(44125..44721)
                     /locus_tag="ACHK01_001461"
     CDS             complement(44125..44721)
                     /locus_tag="ACHK01_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096434.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDDAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(44718..45437)
                     /locus_tag="ACHK01_001462"
     CDS             complement(44718..45437)
                     /locus_tag="ACHK01_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO8096435.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(45434..45868)
                     /gene="psiB"
                     /locus_tag="ACHK01_001463"
     CDS             complement(45434..45868)
                     /gene="psiB"
                     /locus_tag="ACHK01_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO8096436.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(45923..47881)
                     /locus_tag="ACHK01_001464"
     CDS             complement(45923..47881)
                     /locus_tag="ACHK01_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO8096437.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            complement(47940..48173)
                     /locus_tag="ACHK01_001465"
     CDS             complement(47940..48173)
                     /locus_tag="ACHK01_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO8096438.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(48231..48758)
                     /locus_tag="ACHK01_001466"
     CDS             complement(48231..48758)
                     /locus_tag="ACHK01_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8096439.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT        12635 a        13033 c        12205 g        11418 t
ORIGIN      
        1 gtaaaagcaa ttctggtagt acggtgataa ctggtcgtat agcaaatggt cagcaaatac
       61 cgctgccaac aggattttct gccagtcagt gctcatggag tgtgagcaat gcagaaaacc
      121 ctcaaggttg gaagccgaat tactttgcag gatcagttgc aacttatgat gcaaatcgaa
      181 ttgttaaatg tggtttttat gatgaataca atttccataa agggacattt agagctgatt
      241 taaccggaaa atgtagttat gtcgtcgcgt gtcagaactg agattaaatt gctggcgctg
      301 ctggcctacg tgccaatccg gtacctgggg cgctcctaaa attcaattca caacgcagac
      361 ctacaacata gctaaaaaca ctcgtaattt aaggctcggg gtccatgcat attgctcatg
      421 gacttacctg aatggctcac cctttggtgg ttttcaacag gtatattccg accaaaacaa
      481 cgtttggtat gtgagtaatt atgcttgggg aaattatgag tctggtggga ccatatcagt
      541 cacatgcctc aatcttcctg gtgctggagc ttaattaaag gggcaacagt aggcatcgac
      601 atgttcctca tcaacaccat caccaatacc gtacatgcgt gttccagtca taatgtatcc
      661 agtcggacat tgaatcggct tgtaatagat cgaaccacca ctcttaccgc cgataaagtg
      721 attcattgct acggatgatt tgtaccaatt acatgctgag taattaattt tattaccccc
      781 tgaccaccga ccggattggc acgcttgcag agaatgattg ttggcattat tttttgcctc
      841 ttttgaggtt agcatgccgt ctccacgcat ccgtaaaatg tccctgtcac gcgcactgga
      901 taagtacctg aaaacagttt ctgttcacaa gaaagggcat caacaggagt tttaccggag
      961 caatgttatc aagcgatatc ccattgccct tcggaatatg gacgaaataa caaccgttga
     1021 tattgctaca tacagagacg ttcgtttagc agaaatcaac ccccgaacgg gtaaacccat
     1081 tacaggtaat actgtacgtc ttgaactcgc ccttctgtca tctctgttca atattgctcg
     1141 tgttgaatgg ggaacctgtc gtactaaccc ggttgaactg gttcgcaagc cgaaagtatc
     1201 ctcgggacga gatcgccggc taacgtcttc agaagaacgt cgcctttctc gctatttccg
     1261 cgaaaaaaat ctgatgttgt atgtcatttt ccatcttgcc cttgagacag ccatgcggca
     1321 gggcgaaata ctggccttac gttgggagca cattgatttg cgccacggtg tggctcattt
     1381 acctgaaacc aaaaacggtc actcacggga tgttcctctg tccagacgtg cccgtaactt
     1441 tcttcagatg atgcccgtta atctccacgg caatgttttt gattacaccg catccggctt
     1501 taaaaatgcc tggagaatag ccacacaacg acttcgcatc gaggacctgc attttcacga
     1561 tctacggcat gaagcaataa gccgcttctt cgaactgggt agcctgaatg taatggagat
     1621 tgctgcaata tcaggacatc gttccatgaa tatgctgaaa cggtatactc atcttcgtgc
     1681 atggcaactg gtcagtaagc ttgatgcccg ccggcggcag acacaaaaag tagcagcatg
     1741 gtttgtgccg tatcctgccc atatcacgac catcgatgaa gaaaatgggc agaaagcgca
     1801 tcgtattgag atcggtgatt ttgataacct tcacgtcact gccacaacga aagaggaagc
     1861 agttcaccgc gccagtgagg ttttgttgcg tacactggcc attgcagcac agaaaggcga
     1921 acgtgtccca tctcccggag cgttacctgt taacgaccct gactacatta tgatttgccc
     1981 tctgaacccg ggcagcaccc cgctgtaaac atcattcaaa acacatacag aaaggcgagt
     2041 tgcaacaact cgccaaacac attcgatctg catgatcgaa attattcgtt tcaccagaaa
     2101 aaacaccgaa gatacaataa ccacatgagc tgaaaagact ttttcagtcg gaggaacaat
     2161 gagactgaat acgaccggta ttgcggcccg aatgatgctc tcgctggaca aaaaagcaat
     2221 cggcgaaaaa ctcatcaacg gtcgtcagat aaacccgaca cctctgcagg tccgttaccc
     2281 ccaaggggta agagaagttc tggggataat gagcgaacaa ttagccatat ccaccgctga
     2341 tttaacccga atcctgcttg aggatgctct tcataatatg tttatgcctg cggataacac
     2401 cacaggaaac ataataagca gaattgaata catcatgctg tcgcatgata taaatgcgcc
     2461 attactggca acattgctgt cgccatggaa tatccgctcc acggttatac aggacccggc
     2521 cagacttgca gactatcttt cagctgacgc tctcgaacat cttgctgaat gttttaatct
     2581 caatccggac tggctgaatg gccatgaaaa ctatccgata gcgttgtccg gtgaatggcc
     2641 agatactgct gataatttca gaatgcttat tagtgatagc agcaacactg aagttatttt
     2701 ctggcatagt ttccccttcg caggaaatac gaaaagagaa tattgcgggg taatattgag
     2761 acagaaaaaa gaaataaatg gctctgtaat atatccggca ctatcattat caccaacact
     2821 tctgaacgat gaaaaaagaa aatggttaac tgaatataca accaggcaga atacaacaat
     2881 gtctttgcga cgcgtcacgt tgagacctgg ccttgccggg aaccttatta cagggcaaat
     2941 actacctgtt agcttattta atacgtcttt attaccctgg taaataccag atagtaccaa
     3001 agtaaaaatt acggcacatc cgtgtcatag cgttgttttg tctaaattct gataaacagg
     3061 aaattaaatg atgaagaaaa atcatattac ccgcaccatc attgcatctg ctgtattatt
     3121 ttcctttaac gctgcagcag cgacgtcata ttttgaagcg cgtaacgatg caatgggtgg
     3181 taccggggta gcatcttccc attatggtgt ggcacctctg gcaaacccgg ctttactgac
     3241 aaagcacaac agcaatgatg atttcagcct tcttctgccg tctgttgggg cacaagttgc
     3301 tgatcctgat gatgtatcaa ataaagctga tgatgttaaa gatgactggg atttgtttga
     3361 cagtgctgta gataaccagc atggcgtaca acaggcagcg gcaaatctta aacatcgtct
     3421 gcaggaattc cgcaatatca atgcagatgc tcaggtaggt gtatcagctg tagcagccat
     3481 ggcaaatgac actctgccgt ttgcccttat ggtcaagtct tacggaactg tcagcgtaaa
     3541 cggaaaagta aacgatgcag acctggatta tctggacaaa gttgccaatg gaacaattac
     3601 cgacgtcgat aagaatgccc tgacctcacg cgcattcggg cgtgcagctg taattaccga
     3661 cgtcggaatt tcatttgcca aagaactgga aacggccgga caaaaatggt ctcttggcgt
     3721 cacaccaaaa tatcagcgtg tggacctgtt taactacaac gtaacggttc gtgattacga
     3781 caaagacgat tttgacggtg acaagtacca caacacgaaa aacggattca acgccgatat
     3841 tggtgcttat acagacctga atgacaactg gactgtcggt cttgttgccc agaacatcat
     3901 tccacgcagt attgatacta aagtcgtcaa tggctttaaa gaaaccttca aagttcgtcc
     3961 gcaggcaacc gccggtgtat cctggcataa tgacctgttt accactgctc ttgacattga
     4021 tctgactcct gccagtggct tcacttctga cagcaaacgt cagtttgcaa gtgttggtgc
     4081 tgaatttaac gcatggaaat gggcgcaatt gcgtgccggt tatcgtcaga acatggcatc
     4141 aaactccgga agcgccttca ccgctggcgt tggtatctct ccatttgacg tcgtacatat
     4201 cgatgtttcc ggcctggtag gaactgacca cgattacggt gcaatggcac aacttcagtt
     4261 cactttctga ttactgtccc cgcccccggc actaaacccg ggggtatttt tatattcagt
     4321 ttaaataaca tgatgaaacc acgttcttct tattcaaaaa cagcattcat tcttttattt
     4381 tctgttttcc tggtagcggc agtgacaaaa gcaaaaagct cgcttcccga tatcaccctg
     4441 gaacaggcaa aagaaattaa tgctgataac accgttattt tccttttccg ccatggagaa
     4501 cgttgcgacc gttcagatat gccatgctat tcggataaaa gcggaatcac tattaccggt
     4561 acagagaaag cacagcagga aggtattaag ttcgcaacaa tcttcagtga atatgatatt
     4621 tactccagca atgcggtaag aaccatacaa actgcaaaat tcttctccgg taaagaaccc
     4681 gtcgttatgg acagtttatc agactgcaat aatgatcttt ataagacgct tgaatcaatc
     4741 gcaagagaat ctcataaaag gaacatagta ataatgacac ataatcattg tttatcattt
     4801 ctggccagag ataggctcgg caagaaattc aaaccggcgt accttgatgc cctgatcatg
     4861 cattacgatg gtacccggct catacttgac ggaaaataca acaaagaggc atagaggata
     4921 tttgaattta cataaattct gatatactca gtaagataca agagtgtacg ggcacatgtg
     4981 cctgtctgaa ttgcaggtta tccggacgag ggccgggaac tgtttcaggg acaagcgccg
     5041 gtacgttcga ttcataaatc gtcgagtcat ccatcatgga tcgattcggc gcggagcgtt
     5101 aaagattacg ctcatattcg ttttatgtct gacgacacat caaccagcca gtatcaggaa
     5161 taatcacctt cagtaaacta tgaggctatt tacctggcca cactggcatt tatttccggt
     5221 aacgcaagta tagccggaaa caaacatcat cctgtctgta ccggagcacc ggtatatccc
     5281 attctgattt aactgctcag gaattattat gaaccgtatt atacctgcgg ggtgtcttgc
     5341 cgcccttttt ctggctggct gcaactcccc ttctcagcat actcctgttc cgcgtaacta
     5401 cggtgctgcc tctgatgtgg cggttacccg gcacactcag tacacattat gggacaaagg
     5461 tgtattttcc ggccaacttc cggtttcaac ggggtctcat cttctgacag ctaacagcga
     5521 tcgcctttcc tttgactggg aaggtgatgc aattgaatta ctgaacgaac tggccagagt
     5581 cagggggatg cagtttaact acaatggtgt ccgcctgccg ttaccagtca accttcacgt
     5641 ccgggatatg acgttttcca atacgttgag acttattgaa gcccagacag cctggagagc
     5701 aacaatccat caatatccgg gcttgttaca ggtcagcttc atgcagccgg agaaccggaa
     5761 aaaatgaaag cactcacctc tctccttttg ccagtaatac tgcttactgg ttgttcttct
     5821 tcccctacca tctcgccagg tggtgtcaat acgccaccac ctgacattaa tgcatggctt
     5881 aaaccacgaa gtgaaaagcc ggatagtctg tcagaaacac gctggaaaat gctgacagac
     5941 gcaggtaaaa cgcttggatt tcgtggtggc aaggctcagc gttcatggga gctgattcag
     6001 gcgctcaacg cccgggaaag cacacttaat gccttatacg atttccggcc actgattagc
     6061 ccggaaggct ggctaccacc ggttattgat gaagcacagg acgtagctca tatcacgcca
     6121 gatcaaatcc ggacctcttc ccgggtctgg accatcattc gccctgaacg ctttgtcagc
     6181 aatccaccgg gctggcgtga ctggcttctc cgggggcttt caacaacagc cactccgggt
     6241 acagaaggca gcgtcgttcc agaagacagc gtgcaacgaa aagtgtggga aaccgcatta
     6301 cggcagggat ggcaggaagg aagacaaaac gccgacctga cgcttgaagc caaccagaaa
     6361 accctcaccc gtgattaccg gggcatgatg ctgtactcac ttctctggcg tcagggcatg
     6421 atcacccgtc cggatgtttc tgatcaaatg caaaccgtta caggtgacgg gaaaaaactg
     6481 gtcactggag atcgtgttcg tcgccttaaa aatcatgcgg aattcaatct tcagaaatct
     6541 cactggcgtc cgcttatagg cactgaagga ggctcccgat gaatattcct gatgagtttg
     6601 gtctttttcc gtttcagcgt ttcactgcag atgaactacg gcatttcttt gtctggtgtg
     6661 ccgcacataa agtcagtgat gtggatctga ccggcgggtc acctgtatct gtcagtcgct
     6721 ttggccgacg ggtgcgttgt tccagtgcaa cgttaccaac cacactcatg tcctccctga
     6781 ttgatgaact gttcggtcgg gaagtcattc cccgcgttct ggcaggcaat ccggttgaca
     6841 ggacgataca gattaacggg gatgcatccg ggcgctatgg tcttaaacgc ggagaacgca
     6901 tccggttacg ctctcacctc attcagggca catctggtgc ggaggagaaa gcaatctcaa
     6961 taaccatgcg tgtcatcccg acagaaatac cggacattct ctccatgaac attgaaccag
     7021 acctgcttga agcaatggtc tgcaaaagcg gtctgggatt tgtatgtggt gaaaccgggt
     7081 caggaaaatc aacgctgtgt tcagcactgt accgttacat tatggacaat ttccccgacg
     7141 ccaaaatcgt gacatatgaa gacccggtgg aatatatcct gggaaatgaa aacgatctgc
     7201 tcccaccgca tcaggcagaa atcgggcgtg atgtggtcag ttttgctgcc ggactgcgtt
     7261 ctgctgtacg acgtaatccc gaaattatcg gtgttggtga gatcagggat aacgaaaccg
     7321 ccgacgcagc ggtacaggca ggcaacaccg ggcattactg cctgagcacc atgcatacga
     7381 aatctcctgg tgaaacgctg gcacgtcttc ttgggctgtt tccccctgtc atcagggatt
     7441 cgatggcctg ggctgtcctg tcactgttgc agttcattct ggttcaggtt ctggtcagaa
     7501 caaatgacgg tggtcgtaaa gccgtccggg aatatatcgt cattaacgat gagcttcgcg
     7561 acaatctttc cggaatgccg catgcagagt gggggcatca cattgatgcc ataatccggc
     7621 aggagaagcg ccgtatccgg gaccagattc tggaaatgta tatccggaat gaagttgacc
     7681 gaagagaagc gattcttttt atcccgccag gggagttacg gtcatgaaaa gagaaggttt
     7741 ctggcgatat gcaacaccgc cagtcaacat ccagggcgta cctctgccgt tcaccatgat
     7801 ttatctgttt atatgcccct ttccctccaa aacaaccttc tggatttgca ccggcatcat
     7861 tttatttttc gttattctcg accgttcagg ctggactgtc aggactatct gtacacgcat
     7921 tttcagcatt ctgagagggg ccatcgcctc cggtcgtcca tggtggtatc gccaccatac
     7981 ggaatcacca cgagactgga ctggcctcga ttaaattctg gagtctttat gtcaggtaaa
     8041 aaaactatcg ttactctgct ccgggtatcg ctactggcat gcccgttact gttcacgaca
     8101 ccatcgtttg caatgatcga cacgccatcc gtgaaggtcg gcttttctcc tgaaggaagc
     8161 gcatccgctc tggtgcttga cacaatcaac agtgcggaaa gctccatcag gatgatggca
     8221 tattccttca ccgacccgga tgtaatgcat gcgctggcaa aagcgaaaaa acgcggagtg
     8281 gacgtccgta ttgttgttga tgacaagggg aatacgaacc gggcaagtca ggaagcgatg
     8341 aaatatatca acctgctgga cattcctctc cggactgtag atgccttccc catccatcac
     8401 gacaaagtca tcattgttga cggaaacacg gttgaaactg gctcctataa cttctctcgt
     8461 gctgctgcac gcaagaattc agagaatgtc gttgtgctca agaatatgcc ggatgtagcc
     8521 gcacaatatc ttgaacactg gcaggatcgc tggaataagg gtacagactg gaaaccctga
     8581 caaacatttc tcccccgatt ttctatcccc ccagccaccc ccggcttccg gcggtggttt
     8641 ttttccgtct tttttcagga gaagcactca tgccgtctgt atcccgctac cgcacctggc
     8701 ttgccgtgcc tgcagatgaa atagaagatc tcaaaaaagc acatcccccg atgaacgggc
     8761 acacgcccgt catctgggac aaggagcaca aactctggtt cgcccgcccg ggggcggacc
     8821 tgtccaggct ggaccgctgg ctgccccggc cacaggacgt ctccatgaac ggcagtgatc
     8881 cggtcaccga gtttgcccag gttctggaga atgccggact ggttctgaaa gaactgccgg
     8941 tcatggacgg gaaaatccac cgtgtcccga ctgccgatga caaaaaagga caaaaaagtg
     9001 gcgcatatcg cggattcctg gacggcagac ctgccggctg gtacagggat tatcgcagcg
     9061 cggatgattc gccgatcacc tggaccttct ccggcggaga acagacagat ccccgcgcaa
     9121 gactccacct gaaagcgcat tcaatgcaac gcagggagga cgcggagcgg gaactgaaag
     9181 cacagtacaa ccgacaggcc gcgtatgccc gccgctatgt caacaaatgg ccgcaggcca
     9241 cagcacatga atacctgacc cggaaaggca ttcaggctgc accgggggtg cgggtaaaca
     9301 acaaaaatga actggttatt cccttcagta accgtaacgg cgcgatacgc tcttaccagc
     9361 gtattccggt caccgggggg aaggatgccc gcatcctgaa agattcagag aaaaccggta
     9421 actggtttgc gctgggcaca ccgcgcaacg gtcagccggt actgtttgcc gagggctatg
     9481 ccactgcggc ctccctgcac gaagccacgg gccttccggt actgatgacg gtggatggag
     9541 gcaacatgat tgccgttgcc gaaaatgccc ggcaaaagtg gacgcaaagc ccctttatct
     9601 tctgtgccga caacgatcat gcaatccggg ttaacaaagg tatcgtcagt gcaacaaaag
     9661 ccgcagaact gacaggagga acggtcatct ttcctgcgtt caccgatgcc gaaaaagcgc
     9721 aggggctgac cgatttcaat gacctggacg caagccgggg acgggccgct tttcagcatg
     9781 tcatcaacgc ccagttagaa cacatcgggg tttccacccc caacagtaac accccggaaa
     9841 tccgcgaggc actggtgatc ggcaacctcg tttttacacc ggtccacact gaggaaaaaa
     9901 ccatgactcc gacagaatat cctgaaacat cacccgatac agggcacagc catgaccagg
     9961 gaccttctct gccgtccgcc acgcagcagg aacaaccagc tgcttccagc agtaacattg
    10021 ctgacgaaac acagtcattt accagtcacg ccactgaaaa taacggcaaa gatgagcggc
    10081 acgctgataa cgttcaggcg caaacggaaa gctctgtagt tcaggactcc cctgcagaat
    10141 caccggcgtc cccggaaagt acaacggcat ctgcccctga tgaacccgct catccggcgg
    10201 aacctccgga aaaagttgtc tcggtggcca cagataaaga ctggcgtgaa tttgaagccg
    10261 aactgtcaca accggaaaaa aacgagtcac aacaggaatc cggcacttca cctgaaatac
    10321 cggcccctgc tgccgccccc gcatcccctg aagacagtcc ctcatctgaa accatgaatg
    10381 aatcagccga gccatcacct tcccctgatg cagtcaaaga ggaaatgacc gtgatgacgg
    10441 agcctgatga caaacaaagc accggaatat ccccggatac tccccgggaa gaggcggtca
    10501 gccatgtaac tgaagacatt ccccttcagc cagagccgtt ccttcctgag tcagatggcc
    10561 ccgaagaata cgaaaactac agcgcatatc aggagctgat gaacaatgaa aatcctgagc
    10621 atctgcagga aaacagtgat atgccgtcgc aaccggacac agaccacgtt caggcagatg
    10681 agtcagaact tcaggccaca acagatactg cagaaccggc tgtccgggat gaacccaatc
    10741 cacagcaacc tgttcagagc acatcacctt ctgataatac ttcatcgttt ctcgacaaag
    10801 cgcgtggctt ttttacgcgt aaaaaaacgg attcacaggc acatgagaac agtgatccca
    10861 cgccctcacc ggaaaccacg acgaccacac cgacacctga cagtattgtc tacgccccgg
    10921 aacgtcctga tgcaccgata tcgcttaatc tggacgaaat cataaaatca ctgaagggtg
    10981 aaaaacgagc agacagaacc gtcctgtaca agctggatgg caaaccagcc ttcattgacc
    11041 gggtaaaccg gctggaaatg gtcgacggtg ccagcaatga cgatcgcagt gttctggccg
    11101 cacttgctgt ggccactaat ttctatggtg gcgtcattga gctgacaggc agtgatgcct
    11161 ttaagcaaaa agcgatgcgg ttgattatcg agcacaacat caatgtgcgc atgaaacacg
    11221 ctgatcagcg tctggagctc gatacattac gtaaggaaat ggctgtcggt aaggatgctg
    11281 ttgtcaccca tcagccaacg ccggaactta accggaatac ccctgaacaa ccggttgtcc
    11341 ctgaccccgt tcaggaaaaa gaagccacac aatccccggc gtccccagtt gcccctgaag
    11401 catcaaccgt cagcaccgtc cctgcatcat caccggctga acctggcaaa acggcagatg
    11461 cggcacaagg tgaagaacca ccaggaaaac ttcgcccggg agagagcgtg actgccgtac
    11521 tgcacaattt tggtcgtgct gaatatgcac ccggcaaagg agagagtttc tttgttgagc
    11581 ttaaaaaccg cagtggcagc aaactctact ggggggaaca actggaaagt ctggtcaaaa
    11641 accatcagaa aggtgacgtg gtgacactga cacttcagaa ccgcgaacaa ttcattctcc
    11701 ctggtgagca aaaagcacgt ttccggaata agtggtccat ggagtcagtt accaacggga
    11761 tctcggtatc acatgataat ccggataaag gtcagcgtat tcaggcgatt ccggtcgaaa
    11821 cctttatgaa agtggctgcg caaatcagcc aggggtggcc cgaagagatg aaagcgttac
    11881 gtatgccaga aaacgtcggc tcacatctgt ttatcggtga agaccgacat ccggtctccg
    11941 cgccacagaa cgcaaatcag gtaacagaaa taaccagcgc agcccctgac aagctgacac
    12001 ctgttctggg atcagtggat aaggatacgc gtgaactcaa cctgctactt gtccagagtg
    12061 ccgatgaaca tctgcagggt gtggtccggc tgaacggcac attgtatccg gcactggcca
    12121 ccccatcggc cgacaacagt cagttggtta tcaatgcgct gaccgacaaa ggtttacgct
    12181 ttgcaggtta tggtgaagcg gtaaaccacg atgctgacag cacgaatcgc cccgcaccgg
    12241 agcttatgca gtttcatctg aaaacccggg aagaaccgct ttttgctgct gtctacacgc
    12301 cggaaaaaca acctgatgcg ttgtaccgga acctgggctt cgagcagtca tggcagcagt
    12361 ggtccaacag ccaaaagccg gaggacaggc aggaaaaaac tctgcatcag gatctctccc
    12421 acagccccgg ccgatgagcc ggtaaggaag ccatatgaac atgcataagt ctctggttgc
    12481 cgtgctcggc cttatcctga tttcatccac ggcaagtgct gcaacctgtc gtgcgggtag
    12541 cgcggcacag gccggtagta atacaggata tgagcgagca cgtgcagctg cggatgcatg
    12601 gtcacaacgt gaaaacgatg tttcctcatc cctgcagtcc tgtctgtcac gaatcaaaaa
    12661 aatcagcata aacctccccc agttccccag ccttgacgac attctgtcgc agctggaaaa
    12721 ccaggtctgc gacgcggtcg tggacaaggt gaatgaaaag ctgccgggga atattgatcc
    12781 ctggaaggac tacaacctat gaccgaaaat accgagctat cctcgccatc acatccggca
    12841 cctgacccgg aaatactgaa gagcacaatc agggctatga agcagtcaga acagcgggca
    12901 aataatgtcc ctgcgctgat aaaagcgctt ttatgtacgg gcacatgtct tcttatcagt
    12961 attactggca acgccattca gtactggcac tcaacgaatg tggagcgcga atatttcgct
    13021 acagacaatg gtcgtctggt caggctggca ccaacaaatc agccagcatg gagtcagaat
    13081 gacgcaatgg cctttggcag ccaggcactg gcgaccgcat tcaacctgga ttttgtgcac
    13141 tatcgttcac agatttcctc cctgtctccg cgtttttctg acgaaggttt tgccggatat
    13201 gtgaatgcat tacaggcatc taacattctg gagacgatca agaaagagaa gatgaatctg
    13261 accgccacga caggcgctgg cgttctggta cgacagggac aaatgtccga cggcgtatgg
    13321 ttctggacat ttcagtatcc tgtccgtatg cgtctggtcg gccagacaac cagcaagcca
    13381 gaacagtctt ttgttttcga aatcaccatc cagcgtgttg accccagact gaaaccatcc
    13441 ggtatggaaa tccggcagat gatctcccgt aacgccggac cgaactcctg agataagtaa
    13501 tatgcaatca cgttatttac tgagcacact gctcctggtg tgcagcgccg ccacgtctgc
    13561 cgataacgca ggatggcaga acgcacgcac accacaaacg acaaattctg ccagcgatca
    13621 cgttcagaac gcctcccaaa acaccggagg tgttccggca acactggtaa aaggtgagct
    13681 gcctgccccc gggcaggcca gcccgctggt acaggatgcg gcaagacttg attctgagct
    13741 ctccgccgat gagattcgct cactacgcag tctcatggcg gataacgagc gggccatcaa
    13801 tgcgcccatc accagtgttg taccccgtat cagctctctg acagttaatc tgtcccccgg
    13861 tgccagcctt cctcttgttc gcacagccat gaacaacctg agcgtagtga catttaccga
    13921 tatcaacggt tcaccatggc cacaatccga tcctccttat aacgcggcac caaaactctt
    13981 tgatgtgcag tacaacgaga acatggtgac aatcacacca ttgcggccat gggcatccgg
    14041 aaacatttct gtctacctga aaggtctgtc ggtaccggtc atcctgaacg taaccagtgg
    14101 cgaaacagat acgccgtcat ccagtcagga aatggacagt cgtctggatc tgcgaatacc
    14161 acggcaggga ccaaccagcc cggttgtctc catcccgacg gataaaatcg ccctgcatga
    14221 tgcaacatta caggcatttc tggatggtat tcctccccgg gacccttcag ttaagaggct
    14281 gaaattcaca ggtaatgtcc cggatacaac gatctggcaa cacggcgatg accttctggt
    14341 tcgttcccgg gcgatattgc gcgatgaatt tgaacagacc ttgtcctctg ccgacggaac
    14401 tcatctctgg aagcttcccg tcaccccact actgactttc tctgtgaatg gccagtccgt
    14461 tcacgtgaca ccagaactgg agtaatcatg agtgctgaac aggatgccgg taaatccgga
    14521 aagaaactgg ctgccctgct tgggctgagc agcatcatct tatttggcgg cagctatatc
    14581 gcgttttcca aagtatcagg gagtaacagc gatatgcagt cagccgtgaa cataaacagt
    14641 gctgcaagtg gcggtacgcg cagcgttacc gaaacgcctc actatcgtga actgttaaga
    14701 gcggataatg aacgaggggc tgcggcggca gcccgcaata atcagacgtt cattgccagt
    14761 cttccgcagg ggcttgatat tccggacact ccggaaaagc aacaacagcc tgcagcaaaa
    14821 ccggaaaact atgcttatcg ccaggcttcc ggcacaccac aggaggatcg agcagccagt
    14881 gaaaagcgta tggaacggct gcaaaagctg attgtaagaa taaaagatca gcatcctgcc
    14941 ggtagtacac caaccatagc aacgactatg tggaataaat ctccggcaga aacaacaggg
    15001 cagaatggta cgcagcaatt cgcattacag aatgccagcc tgtccacgcc tgtagcagaa
    15061 aaagggatac aactgatacc tgccctgacc cgtataccgg catatattga tactgcagtg
    15121 gattctgata acccgtcatc caaggttatc gcaaccattc ccgctggccc ctgggcaggg
    15181 gcaacgctgt tcagtccggg agtcaaactg gtgggtaacg gcgtggagat ccatttcgac
    15241 cggatgagct ggaacggcat ggaccttaag gtcaatgcat atgctcaacg ggaagacaac
    15301 ctgatgtcat ccgtggccag caatgtgaat acacgctggt ttaaacatat cattctgccg
    15361 tcagtgttag gaggtgtggg cagtattggc acactttata aagatgccaa cacgcaggtt
    15421 attcagggta actatggcac ggttaccgga cgcgtgggaa tgcccagtgg tgaagctgtt
    15481 gcgggcgtta ttgccggtgg tatggcggaa agaggaagtc agatattaac ccgccaggca
    15541 gaaagtgagc cctataaaca ggtagaagtg tatcagcatg aagtcgtatc aattctgttt
    15601 gttgacccgg taatgacaaa cgacgccagg tcatcttccc tttccagtgg catttcccct
    15661 tcagttaatc gtacatcaca ggcggaacaa cgctcgcagg cacgaatgca gacagccatg
    15721 gagcaaagaa aagctgtgat gcaacgtcgg tatgatgaac aaccggagac accataatga
    15781 aacctgaaac agaaatagat gaaaccggca gcttcggtga acccgaagag aaacccgccc
    15841 ccttctggaa acgttccgtc tgggggatat ctgtagcaac atgggggcta tgtgcagtcg
    15901 tgttgctcgc agccatatgg tatctgttcc tgagagcgcc atcggaaaca ggcatgcctc
    15961 cgtttaatga tgcggatgct ggcgtacaga cctggcaaac cacacaagaa tcatcacctt
    16021 ccgttcagtc cactgagact atgactgtcc ggacaggaga agatatgagc cagcttgccc
    16081 gggatgtaaa aacagagctg gataaccgcg atgaaaaaat tcaggcaaca ctgaatatgc
    16141 ttcacgacag cattaacaag ctgggcgaag ctataaaaaa ggatgaagaa tacgcacagg
    16201 aaacacgccg tcagctggat gatatccgga gccgcctgaa cggaataatg acacagaaaa
    16261 gcgtcactga atcctcttcc acaccgcatc cagcaaagaa aaaaacgtcg tctgtactta
    16321 acggaatgaa aatcatgtcg atggaaactg gtatggcctg gattcgctgg cagggtagta
    16381 cctgggctgt cagagaaggt cagacgctgg gcaatgtggt gatccagcgg attgatccga
    16441 ctacccggac catcatcacc agtgcaggga cgttacgctg atgaacatgg atgccctgac
    16501 cgccattgag aactttgcca gcagtatttt cagtgccgga atggatttcc tgtttacctg
    16561 gggagaattc atcggggtga tcagcatgat taccctgttt gcccgcgccc gttcagccgg
    16621 tccggtaaaa atgtctcccg gaaaatttat tgcggggatg ttaaccagct gcatgctcgt
    16681 atcactcccg gcaatgatta acgccggagg cgtacaaatg ggattcaggg ctgactcatt
    16741 tggcccgatt gcatatgtac aacctcagac gtttggcgct gctgctggcg cagcaaatgc
    16801 agttctgtcg ctggccaagc tggccggtgt tggatttgta atgaacggga tatccatctg
    16861 gcgcaaggcc ggactcgatg gacataccgc actgagtgcc agtgaatccg tcagtaaggg
    16921 aaacgtcaaa tttattgccg gcgtcctgct ggtctttatc gatcgtgtcc tcaatgccct
    16981 gctggcaagc attggaatcg tcttctgatt tttttcacct tcatataaac cactgatata
    17041 tccatttaag gagaaactat gtcacgtatt cgtgtcgtag cggcccgtct gtacgccgca
    17101 tttatccacg cacaactgtt tgtaatgtca tgccggagca aactgactaa gttcctgctg
    17161 ctcattccca tgatgttgct gcccaaagcc gttcttgccg atggagatct tgccgatatg
    17221 gttcgcaatg tcgaacaagg tgccaaaaca gcacagtcat catcactgac tattgcgcag
    17281 ttcatcggcg taattgcgtt tctcgggggc ctgattgggc tgaaaaaagt tgggaaacaa
    17341 ggggggatgg gactggctgg ttgcattcta tctatcgtca ttggggcgct acttgccgcc
    17401 ggacctgaaa tgatgagtcg cagccagaaa cagttgggga tttcctcaat ttccattggc
    17461 taatcctgtc cagggcactg gcatttatcc ggtgcccttc ctgttcggca atacaaggag
    17521 gcacatatgt ctgcctggag cccggagcac ataacgttac gacgaacaga aaacggcaat
    17581 gtgattgccc acgacagtcg ggacgatcag gaattcattt ttgcagaatg ctgttccgat
    17641 gaaatcgcaa acatcattct ggaagccgtg aaacgttaca ccagtacgga gcctgctcat
    17701 gcaacacagc attaaagact tgtggttgta tccctttcca gaaattgatg tggttcatac
    17761 acaggagcca ctgttaccgg aaccggaatt aacaacgccc ggtcgatgta tctgctgccg
    17821 gcaaaacgtc aggcaccgtt tcagactgga tgactcatgg ccactcagac agcttaccga
    17881 cacaatcagt gacacacgag ttcgtcttaa taaggccaca gaacatctgg ataagttaaa
    17941 aaagcgtggt gaaccagtcg caaccggcga aaaggaaaag tacaacacgg cagtaaaagc
    18001 cgctgaacgg gcgcttgagc aggcccgtct gtctgccaga cgtctttcac tccgccatgt
    18061 acagaaagct gaaatcacca gtacagagtc gttgtcagag aaagagcagg aactgtttca
    18121 tgaagacggc ccgccttaca gcttatgtgc tttctgccac gcctggcatt cactgaatgg
    18181 ttatgccgca gcacagggcg ttatggtgtg gctacccgac cttcatccat ccacggtcgt
    18241 ggcccttaac cgtcgttcat tacaggaagt tttcagtaat gataagttcc gggtccggcg
    18301 gggacgggaa gcgctctctg cactgatgca aaacagactg gcggttgagg ataaattccg
    18361 cagtttccgc ccggccgatt tcgctgacgt ttttcgtcgc tatccacctt ccggtcgttc
    18421 gcccctgcgt gaaaaaatga acgggattgc cctgattctg acaccagatt cattcatcaa
    18481 aaaagaatac gttgattgat ttttcttcat gccactccag cgcggggtgg cattaaaaaa
    18541 atcaaactgc acaaacctcc ctcctgtaag tccggagttc cctcacctat gaacataaac
    18601 cgtttaatct tcactatcga agactgtctg aacacgctat cccgtttctc tgtcgcaagc
    18661 agttttgttg agtattgcga tttacgtact gtaataggtc tggacagaca ggatcgtgaa
    18721 cgtcgcccat ggctgaattc gccctatatt gcagccacaa agcgtggaga atatctctct
    18781 gtctttgaag tcagcggtgc tttcagggaa atggatgaag catccgacca gactggccca
    18841 ggttcactgg aatcgctgat caccagcatg agtgacagcc tgaatacggc atataaaaac
    18901 agcggtcata aaatcagctt cgtttttgag cgtgatcctg acatggggaa agaagaaatc
    18961 gaagacatgg tcgccccaca aaagcgctca ctggctaata caggtatcca gttacaggat
    19021 gtggtcgatg agaaagtcac aacactgtcc ccatggctgg tccgggagcg ttgctggctg
    19081 gcgatctgga gtggacccga ccttatcagc aacagcgatc gtaccgccca tgatgagctt
    19141 gtcagaagac tggctgaacg ggtaccgaaa gcacgctttg cccagtcccc ctggcagtgg
    19201 accttatctg cactgaaaat ccgccacgaa gcttttcttg ataatgtgga acaggcactg
    19261 agacattcat ctgatggcct gattctccgg ctactggata tacacgaggt cgggcgtgaa
    19321 atacgtcgtc agacagaacg gcacagcaca ccgcgtaact ggcaacccca cctgcctgaa
    19381 gatgcccagc cagcgggata tcgctggact gacgatgaat ccgtactaca tgctccttca
    19441 ttgcatttgc agttattcaa cactcaggtg accacacagg gcaacctggt tcaggcgggc
    19501 ggactctggc acggaatggt atcgattact ttacctccgc aaaatctgca gacgtttaac
    19561 gaactggtcc gtgccgtccc acgcgcagtc ccctggcgca tcagaatgga tctgatgccc
    19621 ggcgggatga aggctcttaa tctgaagaaa acgcttctga cttacagcag tttcatttct
    19681 gccgttcgtc caatgtatga atccgttatg gccctggcgg caacggatga aaaagaacct
    19741 gtctgcataa tgaccatcat ggcctccacg tggggaaaaa cacgtgaaat atgcacacga
    19801 aaccaggcca ttctgaaatc agccattgaa ggctggggcg tatgtggcac cacgacaaca
    19861 tttggcgatc cccgacgcgc ctgggtgaat accattctcg cggcatccgg aggctccggc
    19921 ccggttcccc tgtatccccc gttgtcacat gccatctcgc ttttcccgct taacagggca
    19981 ggctctgtct ggcgaggcaa agggaatctg atgttacata cagaagacgg ttccgcaatt
    20041 gaggttggtc tggcgtcatc ccaacaaaat aaacatacag aactcgcacc aggcgatccg
    20101 ggactgggta aatcagttct gattaataca ttgtcggaaa tacagatatc atctgctcag
    20161 aaaaacctgc ccttcattgc ctatatcgat aagggataca gtgctcaggg actggttcag
    20221 cttattcgcg acagtcttcc tccggaaaga aaagacgaag ctgtcggtat tattctgagc
    20281 aatgatccgg aatacacccg taaccttttt gacgtgatgt acggcgctaa aaagcctatt
    20341 acgccggaaa agaatttcat gtcatcggtg ctgtgtgctc tctgtgtcga taccggtacc
    20401 ggtcaaccct gtaaccccgg agacacccga caaattatca accagcttat cgagctggct
    20461 tttaaagagt acggtgaaaa taatccgcga ctttatcgcg cctcaacgga agaactggtg
    20521 gattcagcac tgcaggactc gggactgtat gaaaaacatg atgcaacctg gtgggccaga
    20581 tcaacctggt tcgaagtccg ggatatgctc cacaatgccg ggtatatcat ggcggctcag
    20641 cgggcacatt atcaggcaat gccccagctt cccgaagtgt cctcgatgct gggccatacc
    20701 agtctcaggg atgttttcgg tactgttcag cgtgatggtt caaacgagct tctgctggac
    20761 tacatccgcc gcgccctgga gcagggacat aacgactacc ccatgatatc cgggtatacc
    20821 cggtttatga tcaacccgga aacccgggtc atcgctgtcg atctgaataa tgttgccggt
    20881 gacaaaacgc ctgcaggcag gcttaaaacc ggcatcatgt acctgcttgc aggtcagatc
    20941 gccggtggtg acttcaccct gccacaatac agggatgaag tgttaaagca gcttccacgg
    21001 gaataccatg aaatcgctct taaacgtatt aaccaacttg atcaggaggt taagacaaaa
    21061 gtttatgatg aactgcataa cgcccggggc attgacttca tctgggaaaa tctggatacc
    21121 caggagcgtg agcaacgcaa gtttgctatc cgcacggttc tgagtacgca gtatctgagg
    21181 gactacccgg aaagcgttct taagtcagca aatactctct ggttactgcg ctacaaaccc
    21241 gaagatatcc ccgtactgag ggataacttc aacgttcctg aatttatgct gaaacgcttc
    21301 ctgaaaatgc cggaaggccc ggcaccagat ggtagtggtg ttcctgttct gggagtattc
    21361 agggtaaaaa gtggcacgct tgcgagaata ctcaaattta ccgtcggtcc actggagcta
    21421 tgggcgctga actcatcgcc aaaagacagt gccctgcgta aaacactgac aaataagtta
    21481 ggttcagttc gggcacgtaa gattctggcc gaaaacttcc ccagaggttc tgcaacgtca
    21541 ttaattgagc accgggcggg ccaacataac agtgacaatg ttattgaaga actggcctcg
    21601 gagctcatcc gaaaacaggg atacaatctc tgatgtgtac aattcatatc accccacgct
    21661 gccggagacc tgccagaggg caggtatttc ttaaaaaact tgaggtaaaa ggtgacagtt
    21721 tctcattcac tctgccgtca cgtgtcttca ggttacatcc ggcccccaat cccagagtga
    21781 tatacgcctc ggatgccaga acacagggaa aggttacagg catgcggttg ttgttgccgt
    21841 ccagcacaga tgagctgata acaacggcaa tatggcaggt ttctgtcccg gatgaagcac
    21901 cattcgacgt tcagcatgtc atccagtgga tactcccttc accgctggct tcgctgatgt
    21961 ccgatgatac ctggttatgg ccatccgttc ccggaacgcc ggcactgagt gccggtgact
    22021 ggcctgttat ggatactgac ccttccacct tacggcgcgt cagacgtcgt tctttcagca
    22081 tcatcgataa ccggaacagg aaaggttata tcaccaggcg atttcagtgt ttcccactgg
    22141 ccccactacc tgaaaacaga aaatacgaca gccttcctga ccttgtgctc catcccgagc
    22201 aggagacaat aaaatgcaaa gaaaaacatt actcgcagcc cttatagcca ccctgagtgg
    22261 aacggcatgc caggctcatg cttattcagt caccgtcgtc gccagccggc ctgttgaaga
    22321 gcaggtcatc cccagaatgg aagccatcaa agatgtcctt ggcaacatac tgagtaccca
    22381 gacagccacg ggtaccgcta tcaatcagaa ctctgaaaag ctggcaagtg tcattgccca
    22441 gaacggacag gcgacacgtc agcagatgat tttcagtaat gaaactcagc gcctcgagga
    22501 ggcacggaaa agcttcaccg tgccagactc catctgcagt gaatccgcat cagggattgc
    22561 aacagaaagc aaaagtgcct cagcatccgc agcctcaaaa ctcagtaaag gtggcggtgt
    22621 gagcaaccgt agcatccgcg accgtctggc cagcgcagca aactctcccg tgcgggaagc
    22681 ttatgatggc gcggccatac acgccagtta ctgtacagaa gcagaatatg cacgttttgg
    22741 aggaaccgcc gtttgtcctt ctgtgggcga aataccaggt ggagacagtc aggtacgctc
    22801 gatttaccac ggcgcaggta cagcggacac acctgcagca ctgacatggg atcagaagca
    22861 aatagatgca gcgacggctt acatgaaaaa cacttcccgc ccctctgccg gtcgtgctct
    22921 gggtaaagga gaagtgaata cccagagtgg gcgaacctac gtcggactcc agaacgaata
    22981 caacggcatc attgattctg cgtcgaatcc ccaactgacg ctgattgctg acagcacgcc
    23041 taatgaaagc acacgtaaag cactggctga aacactgcag tcagactcag cggccgctta
    23101 cttcgaccag gtggcttcac cagaagcaaa agcacgggga tatatgagta cccgtgagtt
    23161 tgaagctttt gaggctggcc ggcgctacgc caacacagca tacctggtgg atttacagga
    23221 aatgcaggga gataacctgc tccgtgaact ggtaagaatc actgcgcaaa tgaactggca
    23281 actgaacgac ctgaaagaac agatccgcca gggtaatgtc atctctggac agcaactggc
    23341 tctgaccgcc cgccagtatt atgaaaaaca actcggtagc cttgagaaaa caatcaatca
    23401 ggcaaacgca cgttgaaagc tgacgtcaga acaggactga aaaaatgaat aagttaccgg
    23461 aaaattcaga ccagacgaaa aacgtcaaaa aatcaccatc cataacccgc atagccaccc
    23521 gggggattat taacattttt gttcctctga tggagactcg cctgataacg aattctgtac
    23581 agcatatgct ggaacaacaa aaacgcagac tggacagatt tcgtcaggta aataataaaa
    23641 aggcgcaact ccacctcacg tgggaagaag ctctgcaggc cagccatatg tcagtagacg
    23701 atctggatcg tcgtttccgc cggcgcagaa cagtatggcg attctgttgc tggagtctgc
    23761 tggccatcgc tttgtttctc agtggtatgc tctttgccgc ctcttcatta cctcttacga
    23821 ctctggtacg cgccatcagc actcttgtgc tgatactgtc aggcgtcgct ctttgtgctt
    23881 ccagagcact gattgttaca taccgcctct ggcagttgca tgaacgaaaa gtcagcgaac
    23941 ccgagcaggg gactttccgt gattttctga acgaccgcaa tggctggcgc aatgccacgt
    24001 taattgccgt aaccagtaaa caatactgat cacgtccaga tgccagtacc actggcatgt
    24061 cgccatcccc cttaattaaa tcctgtgtgg cctttgccca tacgcaattc tattccggag
    24121 ttactgtgaa aattttactt cgcgcactct gtgcaggcct tgctatatcc tcactgccgg
    24181 caatggcttc agtgacctat caggatattg tgtctgccgc aaccaatcct gacgatctgt
    24241 ctcgccaggc gctggtcacg atattcggtg atgtagtcac caatccactg tcaaccagcg
    24301 cccccactct gatcggaagc atgttcgggg cgttcaacag tatcatcgcc gtacttgccg
    24361 ttgtctggtt tatgttcatt ggtatccgtc atgttgtgcg ttcggggcat cagggacagg
    24421 tattcagcac cggacgcgat atcgtcggca cactgagtgt cgttgcaggt tttctgatga
    24481 tcgttccaac cggaaatggc tggagtatcg ctcagctcat tatgttatgg ggcgcatcga
    24541 taatgggggt tgggtccgcc aatgtgatgg tgcaacttgc cgctgataat atcgcaaatg
    24601 gttattcaat gactgtacag ccagttcagg catccacaca aacagcagcg cgtggcattt
    24661 ttgaaatgga actgtgtaag tatgcggtta acgcaggact aaatgatttt aatcagacgg
    24721 ccaaatcctc gacttcactg atgacagaat cagctaaaac cgccagtggt aattacactg
    24781 tcacggtatc caatggcagc ggtatctgtg gttcagccag cctgtcagta gaaggaaacg
    24841 gaacaacaga tcaatcgacg attggaaaat tctttaatcc cttcagtaaa aacgaataca
    24901 gcggtgttat ctcagcacaa cgtgcagcca tggataatat gattagcgac atggacaacg
    24961 ctgccagtga gtttgtgaca acatttctgg aaaaaagaaa ttctggcaac ggaacattgc
    25021 cggatatcga gacacggatt cagagagcag ccgacgagta tgaacgtgct gtacaaaaat
    25081 cgctacccac tgataacgga gagcaatcca gaaaagaggc tcttaaatct tatctcacaa
    25141 cctacgggtg ggtaacgctt ggcgcatggt atcagacctt tgccactgct aaccagcgtc
    25201 tggccgagct ggctgacaga gctccagctg taacttcaat gtcatcactg ggagaagttg
    25261 gtgatactga cttattcagt gcagtcatga gtgcctacaa gactcaatta cagaacacca
    25321 cctatacacc acctctgggc actgttacat cagccaatga gcaacgaatg gccaatgcgc
    25381 aagatcctaa gtctgcctta tctgaaatga tgagcttctt tgtctatcga accaactgga
    25441 tagcaacaga gataagtggc acaggaacaa caagcgatca ggtcaatcca ttgataaaga
    25501 tgaaaaatat tggtgattat acattaggaa caacagagat tctctggagt ggatatacaa
    25561 cagttagagt tctcgcgaca gtaggtgata actctatttt cggaaaggtt gccaatttat
    25621 tctctggatt acctaaagga ttagttgctc tgctggatgc tgcagctccc ccaatatatt
    25681 tcttgttgtt cctgttgttt tgtgcgggtt tttccttatc aatttaccta ccatttatac
    25741 cattcatttt ctggatgaca ggcattggga actggatagt cagtgtatta ataggatgta
    25801 ccgccggacc tctgtgggct gcaacacatc tcggaacatc agaagataga ggaagtcggg
    25861 cagcctacgg ttatatatac ttaattgata gtatgataag gccgcccatc atggtctttg
    25921 gttttttctt tgcttctgtt gccatcatcg ccgtaggcac aatactgaat gcattatttg
    25981 gcgctgccct ggttaacgta cagttcaatt cgctgaccgg tattttcagt cttgctggtt
    26041 tcttgctgat atacgccaga atttgtacaa cctctgtagc tgccatattt gcccttcagg
    26101 cctacctacc tgatcatgtt attaatttcc ttggtgggcg tgagggagcc aacactcttg
    26161 gcagcatggc aaattctgtt aaagaaatat ttattggagg aagtcgcaat atccgacata
    26221 ctcctggaat gaaaacagat cgtcttaaag ataaaggaag tgacgacaag gacggaataa
    26281 aaggataata taaatattgc acaacctccc catcggcatt aaaataatag atagtacgag
    26341 gggaggcatt gtatgaaaga taaaaaagag acacctgaca gatttccaac ttggtggttg
    26401 ttatattatg tattaaggaa ggcttacttc tttttaggaa tacctttctt tttattttgc
    26461 gcactgacat ctacactgat gttatttagt tctcgttatt atggtgacaa cattgaagat
    26521 tatgttgtca cttttggtag ttggttctta ttacttgcgc caggtatatg gatgtattca
    26581 cgtgcaaaaa caaggagaga aaagataaga aaagttgtac aaacaataaa agagtcaggc
    26641 ttctactctc cagagaaggg gtatgaagga ttatctttaa ctcagggagc ttatttcggt
    26701 attgacctta aaaatggcac aatgttgtat gtccgtatct atccaggcaa cattatggat
    26761 gttatcggtt ttgacattca taatttcact cgtactgtaa cagacgacaa aacactggaa
    26821 attcatacga aatacatcaa cctgccaatg gttcctattc catcatggtg tactcatcca
    26881 gaaaccgcat caaacaccat gcacgctatg gcaagtcgag gatatgatta ccctgtcgac
    26941 tttcctcgcc tgattcagga gaagcgtaag gagtgggagc agattgcagg catgcctgta
    27001 gccgaagtat tttaagataa gctccctcca taacagccgg ttcaaatgag ccggcttttg
    27061 ttcattacaa cagataactt atgccgttac cgataaattc gaccaagtgc tttaatcgca
    27121 gcaattttta tttccatatc atcactttga ctcaatctat gtaattcacg agtaatgttc
    27181 tccgcctgac accggccttc acctaatgca tagatcgcag caagtgtaac gtcttttgat
    27241 ttactcttca tcatttcaaa caagaaagta ttagctatcg caggaggcat gctcataatc
    27301 aatcctcttc atgaaataaa caatggcact cacttacaaa caggagtcca ctatcgcaag
    27361 cgactcaact tagcaatatc tctcaatttg aatgaaaaaa ccagatggca tgtgacaggg
    27421 aaccgtccgc atgatgtccg tgaccattta accccataag ctaaccttgt cacaaacggg
    27481 cgcagaacag cctgccagac agacaacaaa acaaaagtcc aagataaaac atgaataccg
    27541 gtatctcaat gcgaagaaat aatctccgta gaaaccccca tgagaaaaat atcatatggg
    27601 agaaaatcgc aattatcaca gccagcgcat tcattatttt cgctgtgctc tggcattaat
    27661 tattttcaac acaaaaagga aaaaacaatg aatctggtag atgcctttgt caaaaaagta
    27721 atatccgggc catataaaga atatggaaaa tggtggatag atgttgaata catttcatgg
    27781 ggcgttcccg ggaaaacaag actgatgttt gagtcaaagg agcaggcact ggaagttaaa
    27841 gaagggtaca aatttttaac gtgagatctc tttttgttaa agggcaggaa gcggtaactc
    27901 cctgccctta gttacgtttc caggcttatc agtcgataag ttcccggatc agttccagaa
    27961 cagcaatcag cgcgtttgca acggcaacta ctgccctgag aacgatgatt aacagattaa
    28021 tcatccgctt ctccatcact ggagaaagaa ccccgttgtt cgctcttaca ctgcctgtca
    28081 caacggtttc tatagtgtca gttatagaca gttcttctcc gaccagagca ccaaaaccag
    28141 caccacctga tgttttggcc aggacttgta acctgttctt cccgctgaac aatatgccgg
    28201 acagaacatg cctcagagct gacatgccga gtatacactt atccagtact tgcagaaata
    28261 taattccaga cgtactatct acaggtcagt tatagacagt tcttttccga ccagagccac
    28321 ccactctgta acggaacagt aaaaaaaccg acctcaccag tcggtttttt tacatctgtt
    28381 gtttctcaaa atccgcccga catgcccgtc acaccgggga aactcatgct ttgaatcaga
    28441 accaaccatc tcaactttgc cgtctcctga aaaaactggc acattcagtt cgataactac
    28501 ccataaatac agagagaaaa tccgatgtca gaaaagtgga gtaaagaatt cgactcttgc
    28561 tttcaggtgg ggagcataga ataaattgcc gatgctctcg agaaacgcag cggaagcgag
    28621 gaaaatgcgt tagccatgct ggcaatgaca gccttcacac tcatgacccg acgtggtatc
    28681 tgcgaaatgc agaacgtggc cccggatggc aaaggcattc ggctggagct aatcggcttc
    28741 aggggaaatg aaacaatacc ggatacgctt cattgaagaa gaaagggcgc aatgaggtca
    28801 gccttcgcaa caaagcggcg gcagaaaacc tgccgccgtg ttgcccgtta acgtttaact
    28861 tcgtaggcta acgttgccac aagcactgtg tttccctgct ggagccggag tccgcaaagc
    28921 gaatccctca ccatccagac aaaacacaga atcgtcacac agatgacgat taacatcatt
    28981 aaaaacgttc gctgtggcat catagcccct ccatgtgtaa gttcacagag atattgcaat
    29041 tgcctccgga taagtaaggg gagattgcac tatgcaaatg cagcatctga tggttggcta
    29101 tcctaagtac taccaaacgg ccgattatgc gttgaggctt tcagtgatgg ctgatatagc
    29161 aacaatgaga atgaaggctc tgcacttctg ggataaacac ggtatttctg cagcttctga
    29221 agcctttggc gtgtcctgcc gcacgcttta ctggtggcgt cagttactga acaaggaagg
    29281 acctgagggg ctaatcccgc acagtaaggc acctctggtg cgacgaaaaa aacactggca
    29341 tcccgatgtg ctgaaagaga ttcgacgcct caggacagag ctgccgaacc tcggtaaaga
    29401 gcagattttt gttcgcctga agccctggtg cgaacagcgc tatctggcct gtccgagtgt
    29461 ttccaccatc ggcagaatga tcgctgccgc acatgataaa atgcggatga tacctgtgcg
    29521 tctgggatcg cgggggaagg cactgcttgt caaaaagcgg tctgccaaac cccgcaggcc
    29581 aaagcactac cgcccggtaa agacaggtga gctcattggg atggacgcca ttgagctcag
    29641 aatgggcgaa ctgcgtcgct atgtcatcac catgatcgac gaatgcagca attacgcgct
    29701 ggcgctggct gtgccgtcac tcaacagtga tatcgtcaat cacttcttca gccgcgcagc
    29761 ccggttgttc ccggtcggta tcagccagat aatcacagat aacggaaaag agttcctggg
    29821 aaacttcgac aaaacgctgc aggaagccgc tatcaaacac ctctggacct atccctacac
    29881 gccaaaaatg aacgctatct gtgaacgttt taaccggacg ttaagagagc agtttattga
    29941 atttaatgag attttactct ttgaagatct ggcgctattt aaccagaagc tggcggaata
    30001 tctggtgctg tataacagca aaagacccca caaggcgctc gcactaatga cgcccgtgga
    30061 atatatttta aaagagaata aaaattgcaa tatgtggtgg acccatacac tccattgcct
    30121 tacggcggtt atcgggctac tataatgttg tctaggcatt acagtagagc ccttggttga
    30181 ttgataaaaa aatctccagg ggctttctgc tgtataccgt ccacaaaacg ctttcggcat
    30241 acagcctcaa gcgcccgcag ctattctaac gactttcgta tctgcaacaa tccttttatc
    30301 acacggctaa ttcctctggt tagctcttat cgcctgcaaa taaattttta acctttccgt
    30361 ttgatttaaa ttccagaaat ggagactttg tcatgtctta caatcgacag cctgtcgcag
    30421 aagatcctat gcagatatgg ggcgctgttg gtgtccttct tatcctgctg ctattcgtta
    30481 tatggttatt cttgccggaa gtggtttacg ccagctgcct gattcttcat accttatggg
    30541 gactggttga ctggggacca tttcataact atgcagcacc acgatacaac ctgcttgcca
    30601 tgaccggtaa caacgccgcc aacataagct atagccagtg ggttaacgtc atggagcaga
    30661 ccataggtat tctgtggatg tacctcttac ctgtgacact ctggtgtttg tgggagtggt
    30721 atcaacatcc gggacaaagt cgtttcaccc gcagaccagt agatattacc cgcctgccat
    30781 acatctttgc ttcgttgtcc cctgctatcg cccctgtact ggcagatggt gatccggaaa
    30841 aactctttca tggtggcaaa cgacctgaac ggcgggttgc tctcaccccg gaagcatttg
    30901 tcgagcagca tactctcatc accaatatgc agctcgatgt tgcagctgca agacgatgtt
    30961 tcatggccca gttgggtaaa cctttaacgt cctggaaaga catggcacca catgaaaaag
    31021 cgctctttgc catatttggt cttcagtatt tcctggatga tcgtaaggca gcattaaagc
    31081 tcatggatac actgaacctc tcatgcagaa ttaaaagcaa acgggacagt gggaaattct
    31141 gtacccctgt ttattcactg gccaaatcag ctttccagag agtgattaaa agcaacggag
    31201 cacagcaatg gctgaaacaa catcgctacg tccgcagtgg gctggtctgg ctgtatgcac
    31261 atgacctgcg gcttacgcca cccaactgga tttggctgaa aggcgtggac aggacgctgt
    31321 tttatgcatt acaccgcgcc aataccacga aaggatttat tgaaggcgct ggcgtggtgg
    31381 ccgttgcacg tgcagaggcc gaagccatgc gttttggcct tccctgccct gaaccctgtg
    31441 ttgatgaagc ggtggaaggc ttacggcggg atatgctcag tctcggtctt atctgggatg
    31501 aaccacaacc agatcgcgac cggaaacgcc gcatcctgac caactggtcc ctcaccgacg
    31561 acatcctgcc ccgcacacct gctacagaca acgaattctg attatccgga gaactgaaaa
    31621 tgacaatcga gtactttgca acacgtattg caaaacatat ttccgtgcag gcaatctggc
    31681 cggatggcag gacagaaatt attgccgtcc ttccacggga tgccctgtca gggctggtaa
    31741 cacgaaataa tcaactgatt catatagcct ttgacgccat gggcacccgt aacagagaaa
    31801 ccgtcctgat tgataaacag gagcataacg ctgaccagat acttacagct attgattcct
    31861 gcctgcgcct tgaatatctg atggaacgcc gcttcagcag aacacctttg ttccgtggca
    31921 taatcgccag cgttgtgctg ttcgtaatgg caacgattgg cttctctctg ttcctctatg
    31981 ttgatcgtgt attctgggat gatacaacgc ctgaagcagt acagacagca ggtgagccac
    32041 gactgcttcc ccctcacctg aatcacactg tgccattaaa tgaaggcatt caactcccgg
    32101 ttcccccgaa aaaagacgtc caggtaccgg agaaaacaat cacaagcaaa aatccggaag
    32161 ccgcagctgc gcgacataat ctggctactg ttctgaagcg gaacgccgat cggggcatgt
    32221 ttaccatcaa cctgtcatcc ggtcatgaac gaaccctgtt cgcatttctc gatcctgcct
    32281 gtcctaactg cagacttctg gagccggcct taaaacgcct ggccagcgac ttcaatgtcg
    32341 ttatctaccc ggtttcagtt attggtggag aggaatccac cgaccgtgtg gcaccactgc
    32401 tgtgtgaaaa agatgctcag aaacgtgctg ccggctggca ccgactgtat tccgcagata
    32461 acggaatgat gacgccatca gaagaaacaa cgcctgcaga tgaaacatgc ctgaaagcag
    32521 ccagagctgc gattgatgtt aacaatgtcg ccttccgtaa gttcggattt gccggtaccc
    32581 catgggtgct gtccgacacc ggctggcatc tgcctacagg aattctgcag gaaactggca
    32641 cgcttaattt attcctgaaa accaccgaca gcgagtctgg ccatgagtga acatcgcgtt
    32701 aaccccgagc ttttacatcg tacagcctgg ggaaatcctg tctggaatgc actccagagt
    32761 ctcaatatct atggcttttg cctggtggca agtcttgtgg ccagttttat ctggccactt
    32821 gcattaccgg catgtctgtt gttcacactg ataacaatgc tggtatttag tctgcagcga
    32881 tggcgctgcc ctcttcgtat gcccatgacg ctggaatgtg ctgacccgtc acaggacaga
    32941 atgattaaaa gaagtctttt cagcttctgg ccaaccctgt tccagtatga ggtcattctg
    33001 gaatcaccgg ccagcggcat attctatgtt ggttatcaac gcgtcaggga tattggcagg
    33061 gagttatggc tgagtatgga cgatctgacc cgccatataa tgtttttcgc caccaccggt
    33121 ggcggtaaaa cggaaaccat ctttgcctgg gcgattaacc cactttgctg ggcaagagga
    33181 ttcacccttg tcgatggcaa ggcacagaat gatacggcaa gaacgatctg gtatctggcc
    33241 agacgctttg gacgtgaaga cgatgtggaa gtcattaact tcatgaacgg aggaaaatcc
    33301 cgcagtgaaa tcattctgag tggtgaaaaa acgcgtcctc agtcaaatac ctggaatccc
    33361 ttctgttaca gcaccgaggc attcacagcg gagacaatgc agtcaatgct gccccagaac
    33421 gtccaggggg gcgagtggca gtcccgggcc attgccatga ataaggcact ggtatttggt
    33481 actaaattct ggtgtatgcg cgaaggcaaa acaatgtcgc tgcagatgct ccgtgaacat
    33541 atgactctcg aagggatggc caaactttac tgtcgtggcc tcgatgatca gtggccggaa
    33601 gaagctatcg caccgttacg taactacctt caggacgttc ccggtttcga cctgtcgctg
    33661 gtcaggacac catcagcctg gacggaagaa cccagaaaac aacatgctta tctctccggg
    33721 cagttttctg aaactttcag cacattcacg gaagcattcg gggatatttt tgctgaagat
    33781 tccggtgata tcgatatccg tgacagcatt cacagcgaca ggattctgat ggtaatgatc
    33841 cccgctctgg atacatcagc gcatacaacg tctgctcttg gacgaatgtt tatcacccag
    33901 aaaagtatga ttctcgcccg tgatttgggc taccgcctcg aaggaactga ttccgatgca
    33961 ctggaagtca aaaaatataa ggggcgtttc ccttatctgt gtttccttga tgaagtaggg
    34021 gcatactata cggaccgtat tgctgtggaa gccactcagg tgcgttcact ggattttgct
    34081 ctgattttga tggcacagga ccaggaacgt atagaaggac agacaacagc cacgaatact
    34141 gcaacactga tgcagaacac ggggacaaaa tttgccggca ggattgtcag cgagggtagt
    34201 acggccagaa cgctgaaaag cgccgccggt gaagaagcca gggcgagaat gaacaacctg
    34261 cagcgtcagg acggcatatt tggtgaatca tggattgaca gcccacaaat cagcatcctg
    34321 atggaaagca agataaatgt tcaggagttg attgaacttc atcctggcga gttcttttct
    34381 attttccggg gagaaaccgt accatcagca tcattcttca tccctgacga tgagaaaagc
    34441 tgcagcagcg atcccgttgt tataaacagg tatatcagcg tggatgcgcc acgcctggac
    34501 cgtctccgcc gtcttgttcc ccgtactact caacgtcgaa taccgtcacc ggaaaatgtc
    34561 agtgccatca ttggcgtcct gactgcaaaa ccctccagaa aaagacgaaa aattcgcaca
    34621 gagccgcaca cgatcgtgga tacatttcag caacgcattg caggacgtca ggcggctatg
    34681 gcaatgctgg aagagtacga cacagatatc aacgcacgtg aaagtgcctt gtgggaaaca
    34741 gcggtgaata cgcttaaaac gaccacacgg gaagagcgac gaattcgcta tatcacgctg
    34801 aacagaccag aactacctga aacaaaagag gaaaatcaga tatcagtgag agcagaaaga
    34861 gcaggtataa acctgttaac gcttccacag gacaataacc acccgacagg gcgtcctgta
    34921 aacgggtttc atcataaaaa aaccaaccgt ccggactggg acggaatgta ctgaacagtg
    34981 gccgcgccgg attctggcgc ggcctttttt ttaacctgga gaaggcggtt cccagcgttt
    35041 atcctgctcc ggcggaggca gtgatgcgtc atcgttgctg ttcgccgcga tataatcgcg
    35101 gtagtagcgt tcacttcctt cacggtgatg atccacgtcc ggtcgcgtaa tcctgtagtc
    35161 ttcgtgaccg gtacggcctg tcacattatg ccaggcaacc atttcagcaa agacctggtt
    35221 aaattgtttg tcgtttcctt ctggttcaaa ccccatgcgt ggatcacgcc cgaataatac
    35281 cggtgcaatc aaatccagtt tatctgccag cgcattgcgg tcatggtgat tacggaaaac
    35341 aactctgtca ccataatccg tacagacaaa ctctcccgtc cgacggtcac ggaaacgaac
    35401 actcccgttc ttctgcagac gggcctcgag atcaccggta ttaccgtata ccggcgttcc
    35461 gcccggttca caaagcacga cacagcggtc tgtcgtcgtt gtgcgtgact tgtctttacg
    35521 gcgatcgcga taatcccagc ctctcaactg cgatacagca gccctgtctc cctgagcagc
    35581 ctgaatttcg acccactgac ggtaagaggg tggataccat ttcccgtcag caataagttt
    35641 ctgtcgctca tccctgatgt cttctttcag cctgatcaat gcctgcattc gctgaacttc
    35701 tgcaatgtga taatgcagct tacgtaatgc cggatcatcg tactgcgcac gaatgtgtga
    35761 cttccgcaga cggcatgcct gatgaatttc ccgacaacgt tccccataac gcagatccgg
    35821 tttacgccat tgttcacgcc atgccagata gcgggcacgc aggtcagcac gggcctcagc
    35881 cctggcttca cgtcgctgcc ggcgcagcat tgggtcacgt ttactgctga ccccgtacct
    35941 gtcactgaca gccagctccg ggttatagcg attttcaggt tgcaccctgt caaacaaatc
    36001 tgctggagca ctcacaaaag gtccggcctg tggctccgca cgccccagtg tcagatcagg
    36061 atgaatgctg ctggctttca ccggcgtttg ctcatgatta aaagcatcaa cgacaacaag
    36121 cccgtgatgt tgtttctgta gcatcagtcc ctgttgtgca aacaactcat gacaacgctg
    36181 ccaggtaaac tcaccctgtg tgatcatttc ttcaatctga gggctacaat ggctgatgct
    36241 gtatccccac agactttctt tgtcagaaaa cagttcgcgt ttgatagccc cctgcggccc
    36301 cgtacctgta cgcatacggt gccattcttt atcggtcgca ggacagctct ccatacgacg
    36361 accacggaag cgttctgttg gcgtaacctg gcggaaaata tctgttggta caggctgcca
    36421 gccaccatcg agttgattca cattgtcgag cgtcagcagt gaccatacgc tgtcagcacg
    36481 aacgggagtc tggttatgat cataaccgtc acagataacc aggtgaccat gctgaacacg
    36541 catccataat cccgcttcag ccagtgtgcg gtgaatagcc aggcagtttt ccagccgtcc
    36601 ttcccgtgcc atttccggca gacgttctcc aagatgacga caggcatact gctgcagact
    36661 ttctgtttca gcgatttttt ccgggcgaac atcagctgca atgaagtccg gattatagcg
    36721 tcctggtgct tccacctggc tgaaaatgtc ttttggcact ggcgtgtagt cacccatttt
    36781 tttcatgagt ttttctgcac accaggaggg accaaacgag tcaagctgta caccttccct
    36841 gttgcgatcc cagccatcca tcaccagaaa ttttccgtcc atctgagaca ggtacagacc
    36901 atcttccgca agacgacgat gcagggataa ccagtcatgt acaggttcac tgcgtaaacc
    36961 agcgaccgct gtctgcgcaa catactcacg aaaagtttgt ttttttccgc gtgtccaggc
    37021 gttctggcga tcacgttcaa cggcagtttt gcgaacgata cgatttcccg gcgcatggac
    37081 ccagcaaccg ttatccggag caaaaccatg cttcagttca agctcacggc aggcacgact
    37141 gagtttttcc tgactccatg ataaccggtt cagataaccc gtttcagggt gtacccggtt
    37201 aacagccaca tggacgtgca gattatctgt atcagtatgc acggcagaga catactgatg
    37261 gtcggcaagg ccgagtgatt tcagcgtatg acgtacactg tcataaatct gttccggacg
    37321 cgggctttca tgtgactgcc aggaaaggat gtagtgaaaa acagggtcag tgtcatcttt
    37381 tgcatagtgt gcctgccggg caatgtactc catatctgca gcggcagttt caagagaagt
    37441 acagttatga aagcaggtga caccataaaa gttgacccat tcgcagccgt ccttcatgac
    37501 atcaaccagc gccacgaatg actcatttcg aagacgtgtc gcataatcaa caaggcgact
    37561 gaaacggctt cggtgcggtt gttcagcctg cgaagaagaa gataaatcca gttcctcatc
    37621 ggtcatgtca tcccggacag agacgtagga taccaggtct tcgaacgaag acttgccgtc
    37681 ccttctcttt ttcggaatga ctgcattcac cggagaatcc tcaaccttcc attaactgtt
    37741 tacgcaatgt atttgtaagt tcggttatgg caacaagcac ttctgcgtat tccttgtcac
    37801 cggttctttt tccctgaaca aacagatgct tctgcatcct tcccagtctc attaactcct
    37861 tcagaaaagc atcatctgta cgggagttaa ttcgcttatt cagtgctgca tttcggatgt
    37921 aagcagatac agtaagtccg gaatcttcag cattcttaca gatagtttca tcctcatccg
    37981 gtgagaaccg gaccgctctc aggatcgtct tttgccgtct ttcactacca cttctttgac
    38041 gtttatcact cacggtcagc tccttactgg ggtgtcgggg cgaagccctg accaggaggc
    38101 aattgtataa tcgcgcgtgc gcggttatac aattgcacat cctgtcccgt ttttcaggcc
    38161 attatagcct gttatttttg tgcatcatag tcttatgcag acggcagagg cagggcgcag
    38221 ccctgccagg agtcagtttt tgatgataaa atcagacttc aacatgaaac ggagagaaag
    38281 tgccaatggc ttctaaaaaa ttttactcag atgatgacat ccagctggca aaagctgctt
    38341 tatcggaatt gccagatctc accgctcaac ggaaaacatt gcgtgatttt cttgatgcca
    38401 ttcgtgatga cattattgtc ctggtaagga caaagggtta tacactggca gatgttcgtg
    38461 acacgctcaa aaatgcaggg tatgaagtag gagagaaggc acttcgtgac attattcggg
    38521 aagcagaatc gaaaaaacca tcccgccgtt cttcctctaa aacagcacct aaaaagacag
    38581 attcaggcag gaaagatggc atcgatatga acaacaattg atgtgacaat gtcacatcgt
    38641 gttctgagat tatgatgatt acagaaaaaa aagccccggc ataaatacca gggcttttct
    38701 gtttaatcag actcgtggcg gcaatgcacc tggataatgc cgtgcaataa tatcattcag
    38761 tttttcaaca aggtcagaac gcttaaagac aatgtgtacg gtccctttct ggtagccacg
    38821 caccgtaaaa tattcccctt ctaacagctt accgacattg tcgccgttcc cgagaaattc
    38881 accatactgc gccccgatac tgttcctgaa gtcaggaacg ttgcgcccct ccagcagata
    38941 aaacggacgt accaggtcat ccagtgcctc ccgtccgctg cagtccagcg tcacacgccc
    39001 gttactccag cggtacagca ggttttcaat aatgattttc ttacccagac ggcagggatt
    39061 attggttctg taatcccatg acaatttccg gtagacatca atcagtccct gttcaaatgt
    39121 ctgcatcttg caggcattca gatggcggaa tgttgccagt acattgtcca gggtgatttc
    39181 aggacaggtg tcgctcatca gctggctgtt ccactcatca cgctggcatg aactcatgaa
    39241 ggtgtacatg cccgtatctg tcatcaggcg gtcccagatt ttccggtcgg caagacgggt
    39301 gatattcctt ttcgcttctt ccggtttatc tatgcgacga acggaatcag tcacgcagtc
    39361 ttcaaacccg taaagaaaaa tatcacctga cgcatcgagc agaatcttgc gcgctgtcgc
    39421 caggcattcc atcgcctgca tgaacagtgc aataccttcg ttacgctgcg cgatgatgcg
    39481 gtcgatagcc acagacggaa taacatctgt cgtgccggca gtattcaggt cagcagtggg
    39541 taaagtctga ttcatcacat tgtctcctca tcgtcacgac tgccgggaca gccaggtcat
    39601 ttcccggagg agcgaagctc cttctgccgt ccgggtcttc ggggatttcc ccgcagtgga
    39661 cctttccgac gggggacgct caccacgtca cggggcagtt gtccgggggc agacgcaaaa
    39721 gtttttgacg cctgcggcaa aaagttttgt ggctggtaca tctcccccct ggacgactga
    39781 aacgggatgt ggtcatgtat gtacccccgg aaagtccgtc tgcggagaac aggccgcagt
    39841 gcggcggcag acaggcagag acagctgcgc catcaggcgc agtggcctta cacaccgtaa
    39901 ggtgtgcagg gagcgccgca tacggcgcga ctgaagaaaa acgacggcac aacaatggca
    39961 tgtggtggtc actggcacac cactcccgca caggtgaaaa cagagaaaac cgcacgacat
    40021 ttacaagcaa aaaaagtgaa atttcttcac ttttttacac ttatttatcg cgctgatttg
    40081 gcaattgtga tgataaaata aagtgaagtt ttttcacttt ttcaaaggtg ttttatgacc
    40141 ggctgggaac tgcgaatctg gcgaaaaagc atgctctggt cacgcgaaaa agcggccaga
    40201 gaatttggcg tgacgcagag aacatggcac gcctgggaaa atgcggaaca ggtggacgtc
    40261 accgtctgga ggaccaccca ggcactttca gtccgtgacc tgctgcctca catgcagggg
    40321 atgcgtaagg cagatatcat caggcgactg gaaaatgaac tgggggaaac ggcagaggat
    40381 gtgtgagcgt gattcacagc ctggctgaat gccaggctac tgctcatcag gaaacagctt
    40441 tttcagttct tcaccggaaa gtccggttgc ctcctgaatc cgttccggcg tccagcccat
    40501 atcccgcagc cggcaggcta tttcccgcgc cacttcaagt gcgccttttt gaataagacg
    40561 ttctgcaatc gtcatgctat ttgctctgct cctgctgaca aaaaagcccg gcattttgcc
    40621 gggctgtgat gtttactcag tgagtgattt tcttcagttc gtcatcggaa agtccggtag
    40681 cctgcttaat ctgctccggc gtcatcccca ttttctgaag ctggcacgca atagccaggg
    40741 ctttttcaag cgctccttcc atgcgtcctt ccatgcgtcc ttccatgcgt ccttccatcc
    40801 tgccctcctg gcgacctttt tgctcaagac gttctgcgat agtcatcagt ttatcctcat
    40861 gctgcggcag acgctcagcc agcgcccgca taaattccgg tgatacagag tcccctgcgt
    40921 taacgatgta atgaatcaga acacgcacct gctcgtctga aatttccacc atgacctgcg
    40981 ggagtttgtc cagcagctcc atcatgtcac gatggcggat atgcttcatc agcagcgtca
    41041 gtgcggccat ccggcgatgg ttcatgattt cattgtcatc aacgacagtg atatcaacca
    41101 gcggaaacgg ctttgtgtat attttagccg caagtgccgg attctcaaaa cagtccagcc
    41161 agttcatgct gtacgggtac gggctgcgct caccatgata gaacagtaca ggaatcacca
    41221 gtggcaacgt tttgtgctgc tccagatgtc tctgcatcgc cgccacactg taacgtaaca
    41281 tgcggaaagg catgtacaga ttttcggtgc tctggtgctc gatgagcgta taaacatagc
    41341 catcttcacc cccggtggtt ttcacactcc agaggatatc gctggcgtac tgatgcaggt
    41401 ccggctcaac aaaggtggcg ggttccagct tcagcgtgga caggtcgcac aactgccggt
    41461 actccgccgg aagatgcagt tccagaaaat ctctggcgac gtcgggattc gccaggaacg
    41521 accggaacgc ggcatcatgc ggcgtgggcg tcgtggtggt tttcttctga ctcacggttc
    41581 ctcctggtga gatgtctgaa cgtatcctgc cgcctgcaca ggcagacggc aagggttaat
    41641 cagtggcaga cccgctcaat ttcaagcgcg cggagcaggt ggccccattc gctgactttc
    41701 atagcgggga tacgcacccg cttctgctca ctgcagcgtc tggcgatatg gccgctcatc
    41761 gccaggacgt ccgggttaat ctcatcctgt gccagctcct gacgtctgaa gcgggtatgg
    41821 cggtgcaggg gtttattctc ccgcaggcac tcttcacgca gacgctttac atactcggcg
    41881 gttgcgaaga taattgatgg tttcatggca taatctccat agatagtgat tgagatgccg
    41941 gacttcctgg cggggaagcg ccggcggaga gaggcagggt ttatcgccct gccttttttt
    42001 catttcggac agcgacgcag tgccagcgcg agcgctttca tcttccagcc ttccggcgtg
    42061 cggtacatca ccttccagta gccgtcgatg aacacataat ggaagcgcac ctcttcagtg
    42121 ctttcagtgg caatagcctc cctgaaagaa cggtactcac gcgcctcact gtccatccgg
    42181 aaaccactgt cacgtccgta ataagccgtc acaccgtcca ccgggttctg aagcgtatgc
    42241 ccggcgggtt tgtcgcaacg gggaccgagg caacggatat caccgtgttc aaccagggct
    42301 tcggcctgct gaccggtggc atagtgggta tgcaggatgc gaccgttcca ggtcggctcc
    42361 ccgtgcaggt ggcagtaaac ggtacggaac accccgtcgc ggcaaaaaac ggaaatcgtg
    42421 ctgctgactg tcatgaaaac gtctccttct gctgaatcag gtcgccgggc cgaccggttg
    42481 ctccccggag gggcgaaagc cccttctgcc gtccacttct tcggggtttc tccccgcagt
    42541 ggacctttcc gatggggcgt ccgtcaccac gtcacggggc agctgtccgg gggcggacgc
    42601 aaaatttttt gccgggttta tggctaaaaa ttttgtggac ggtatatctt cccctggact
    42661 gctgaaacgg gatgtggtta ctccagcccc cggaaagtcc acagcgggaa acaggccgca
    42721 ggggtggcgg cggaagggtt gactgcgcca tcaggcgcag tggccttaca caccgtcagg
    42781 tgtgcaggga gcgccgtttc cggcgcgaca ggggcggcgc gaccggaacg gtcgctgcaa
    42841 gcctggtttt tcagacatcg gaccgtagcg gcagcccgaa accgcgccag cgggttcggc
    42901 ggagactggc gggtgcgcca gcagctgcct gtcggagtcg ggcctgacga ccggatgccg
    42961 ccaggcagcc gggagccgaa ggtgtccccg cctccggaaa tcagaacaac agaaacgaca
    43021 taacagaaaa atgcctcaca ccatgacggg caggaagggc agcgccgcag gcgcggccct
    43081 cctgcatttc aggcaggcgt aagcctgcca gggttttccg gcggcctggc aggccatacc
    43141 cccgccggag cgggggagta aatcaccgac ggaacacatg accgtcaata aaggtgaagg
    43201 agtccaggaa taaatcccgc gcatacgcct catagtcaaa atagagcgcc accgtctccg
    43261 gcacgtcagc cagcaggccg gtatcactgg cgaactcaac cgcaaaagcc tcctcactgt
    43321 cagcctcgcc ccaccaggca tcgcggaagg tgtcaaaatc cgtctcaccg gtatcctcca
    43381 cccagagacg ataagcctct tcgcagcctt catcccgtgc caggcggaag ccttcaaccc
    43441 aggcccagtt gatatggcat tcagaggcca tattccccgg gaatccctca taatcctgaa
    43501 acatcagttc aggatcggct tcatcctggt gaagagcacg gcaggcggcg aaaaagtcgc
    43561 gctcatcatc aaacgtggtc aggtcaaacc agcgtccggc gatgcttcca cagttgtatt
    43621 tgtgccaggt tccaacgtat acagcaggtg caacaacaga cattcccgaa tcctccatag
    43681 tcacatgttc tgcgacaccg ggcggtgccg tgcctccata aaagccgggc gataaggcgg
    43741 ggatgcaggg gcgcaaggga agccgcagcc tgacgcacac ggctgacggg agttgcaggg
    43801 tgaggccgcg aaacctgagc ggacgaacga ccggaaaatc acggcagacg ggaaggaggg
    43861 accgggtgac cgcagcggcg aagcgtaccc ttgcatccct gccagcccgg ctaagatgga
    43921 ggcacggcac agcccgcaca cgacgctgag gccacagagc tgaaacccca caaggccatg
    43981 ccccgcatgg ccgggtaaaa gcggagccgc cggcgacttc cttattccgg cgagtgcaga
    44041 ggacacgccg catgacctcc agaccacccc tgatatgaaa cgatcatgat cggcgtgtcc
    44101 ctgcgtaaaa tatacatgcc tggttcaccg gagaatatct gtgatatcca caagttttct
    44161 gtcgcgcagc cagtcctgcg cttcacgcac cacacaactg tccggtttca ccgctttcag
    44221 cgatgatgcc agtcgcttga tcaacatcac cagttcccgt gtctgtatgt ccggcattgt
    44281 ctccggcgga gcagggggac tctcatttcc gacgctcatc ccatgcgctt ccagtgtggc
    44341 acggacaaac caggcgtcat cgaccagttg ctgtccaatc caggccagat cggcatttct
    44401 gtcacgggct gccattcgct cgcccgacag cgccttgctg atatggctgc gcaccagatt
    44461 atccaggagt gcctgcgctt cttccggtga aaagccacct ggtatctctg tgcgcaacag
    44521 ccctttcctg tacagaagct gcagcagatc aacctgcgac cgcacagcaa ctatcagtac
    44581 ataacggaca acattcgcac tggcagtggt ttctgtatca ttcagcagtt cttccggatc
    44641 gttccccccg gtgttgcaga gcaacggcaa cagaggtcgc atccacgcgg gaacctggta
    44701 tttttcgtct gatttcatca tattacgttc tccagcttgt tcgggatcat ccactgctca
    44761 agtgcacgta cctgtaccgg tgcaccacga ccggcctcac cggcttcaaa aatcagccgc
    44821 cagagtggtt cctcctgcca gaaaaaccgg tcaaatgatg acagtgatgg aaaacgcccg
    44881 caccaccccc agaaaatcgt ttccagatca tgctcctcaa caacaccaga ccagcgggac
    44941 aaccagcttc cgaccgtggc gggagtatga aacgcaaggt caatctgcgc ctgagcaaaa
    45001 catgtctggt attccagttc ccgtcgttct gcctcacgtt cccgctggcg gctgtatttc
    45061 tgctctgtca gcgtggtttt ctgacgacga cgctcacctt cacgaaaagg cacggacggg
    45121 aacagacggt gccccgcatc ggatggcagg ggaagaacac agacttcccc aaaactttca
    45181 atcagtttat ccacggcagc cagccacagg agacggttgc catgaaattc ctccttcgtc
    45241 agcgcagggg caaaaaagcg gagatcggtc agcgaaatcc ggcgactgcc attcaggcag
    45301 cgaaagaaag cggtggcata aggccatgct gaaagtgtgc gtccctgacg acgacgttgt
    45361 tctgtgacag ccaccgcctg catggcggcc tgttgttctg cgctgagggg gatcagtgcc
    45421 cgtgaacgtg cgctcatgcc tgacctcctt ctgccatcag gatatggatg atgtcgttaa
    45481 cactgtatcc ctgcgcatcc agtgtcgcgg caagcgccag cgtgtgactg atcgcggcag
    45541 gcgtgaagat atgttgcacc tgaacgacgg cgaaaggctg tccaccacca ttcaccagaa
    45601 ccagcatcca gagctgagag acgtctcccg gcgaacataa tgccaggtgg aaacgttcgt
    45661 gggctggcgt gaaacgaacc tggacgggga aaaagccccc gaactcgctg ccatactctc
    45721 cgttcatcat ccagttagcc ggtgcgtcca ctttacgcat cacctcgtga gtgagattac
    45781 ggcgcatatc gcttcccgca gcacggattt cttcatattc ctgggcattc atggactgca
    45841 gggcattcag tgtcagttcg gtattcatca tcgtatttct ccctctgccg ccccgtctcc
    45901 ggcggggcgg tgtggttgtt catcaggcgg catcagccag gttatcggcg cgttcagtgg
    45961 catccgtctg tggacgtggt gaacacatcc agccaggcac ccagcggttg tctttcatat
    46021 ggaactctgc atgttcagcg gcatcgcctt tcttcatctt ctctgcgtcc cgtgcggcac
    46081 cggacagtcc ggcatcattc agggctgcga taatctgcgg ctttttcagg tgtccgaaga
    46141 agtttgcttt cgtcggctgc caccagtcgc gcatgtgaaa accgatggcg ctctccagtg
    46201 agtcaagcgg actgcgtgac gtgcgaccac actcgcgggt ctggacaccg ttaaggctgc
    46261 atgcggtgca gaaactgagc agggataaca gcacctcctg actgagtgac aggaacgtcg
    46321 tcatgtcccg tgaccatccc tccggtaaca gggccgcaag acgggctttt tctgccatca
    46381 gcgccaggaa tgcggcacct tccttccctg acggcgcatc gctggtcagc gaataatgtt
    46441 tacattccag gctgatttgt gctggtttac tgtacgctcc gctgtcaaac acattcaggc
    46501 agagcgtcca tgccagcagt gccagagatt tgtccggttg ctgcatcagt gctgcctgga
    46561 ctgccagcgt gcgttcggaa gacattttcg tcagcaacgg cagactgatt tcctccactg
    46621 acgctttctc ctgcacctgt tccgtgcggt cagcgtcatc agtcgcgtca tcttcactgc
    46681 gcaactgcac accacgctgg acacatacgt ttccataacg ccaggacacc accacgccac
    46741 ttccggcacg catctccggc gtccacgccc tgaccttcgc catgcagcgc atcagcttca
    46801 tttctgcttc cagcaggtcg gattcctcac actggttttc cagcgcgtcg taacgcgtca
    46861 tcagttcgtt caggcgttct tcttctgcct ccgtcagcac cgcttccggc tccggcagac
    46921 agcggtatgt tccggcatcc tcacggcact caccgacagg ctccatgcgt ccggcgcacc
    46981 attcccagcc ttcggcttcc cgaaggtgtt cagcgacagc ctggagtttt tccagcaggg
    47041 cagcatcgag cgccacgcgg tcgacataac cgtccccgtc atcgctgaac aaatcagtac
    47101 gcagttcgtc aggtgagaaa gcatcagccc ccacgaagcg gaatttactg ttacctgcca
    47161 ccgccacttc actttcggtc accagacgac gaatggtctg tacttccggt ttaccgcccc
    47221 atcccgactg gcaggcggct tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg
    47281 ccagcgcctg acagtgctct gtggtgatgc ggtcttctgc cagcgcatcg aggatgacag
    47341 gcgcaaggtc tgccagtttc agcattcgct gaacgtggcg gggcgaatag cccagcaaat
    47401 caccgatttg tgcaggcgtt ttgccttcct gcgccatcgc acggaatccg gcaatctgtt
    47461 cggcagggtg catatcccga cgatgaccgt tttcagtcat tgatgcagcc gtcgccagct
    47521 cctgcggaat aaccttcacg cgtacaggcc agtcagccgg aatgatgcca cgctctgcca
    47581 gcatgttgag tgcagccagt cggcgaccac ctgcggcgac accgtaacgg tcaccaggca
    47641 gggtatgcac gaccagattc tgcagcagtc cgacgccctt aatggactcc gccagttcgc
    47701 tgacggactc cgcagaatac ggcaccgtgc gcacattcag cggtgattta atcagcgagg
    47761 ccagcggcac gctcacttcc tccgtctgcg ccagcagggc agacaggaca gtttcctgcg
    47821 tttttacaga tttacggctt gctttgcggg tggtttctgg ttcacacttc gttacaggca
    47881 taatcatccc cttataattg cttatgtcct gccggacgtt cgcgcgtccg gcacctctct
    47941 tacttaagaa tcccgaaatc cctgatgtac tggttcatcc agtacccggc acagtcctca
    48001 aaattccagg agcgccagat caacgtgcca tcctgacgga caaccaggcg aaaatgcgtt
    48061 ccctgatcat cctcgataaa gacgttccgg tactgtgcgg caacggcttc cgcctgttcg
    48121 cgggtaaagg agccgtccgg cagtccctga agtattctga aatattcgct catcctcatc
    48181 tctccggtga tgccccgcgc agggcggggc ggttatcaca gtcaggccag tcagaacggg
    48241 atatcgtctg aaaacccgta atcctccccc tccggcggtt gcggctgagg ctctggctgc
    48301 gcggccttac gtccacggcc tttcgttttc gcgccacctt ttttcgtcgc gtcagcactc
    48361 tgtggctgcc cattctgctg aggcttcggt tgcgcctgag cgttctgctg tggtgcacgt
    48421 cccagcatct gcacggtgcc cgtggtctta acaagaattt cagtgacgta acgggtgata
    48481 ccgttatctt cccagctacg ggtgcgaagc tgaccttcga tgtagacctg cgcacccttg
    48541 cgcagatatt cacctgccac ttccgcgagc ttgccgaaca gcaccacgcg gtgccattcc
    48601 gtctgctccc gcatctcccc cgtctgtttg tcacgccagc tttctgatgt ggccacctgc
    48661 aggtttgcca ctgcgccccc gttggggatg taacggactt ccggatcatt gcccagacgc
    48721 ccgacgagga tgaccttgtt gataccacgt gcactcataa gaacctcctg actgaaagat
    48781 gtgagataaa ccggataccg gcgacgggac gttcatgtgc cccttcagag gagcgaaagc
    48841 tcctcctgcc gtccacttca caggggtttc tccccgcagt ggacctttcc gatgggggcc
    48901 cgtttaccgc ctcacgggac agctgtccgg gggcggacgc aaaattttta gccgggttca
    48961 tggctaaaaa ttttgtggcc ggtgcatctc accctggaca gatggaacgg gagggggtta
    49021 cgtctgcccc ccggaaagtc cacagcggga aacaggccgc aggggtggtg gcaggaggga
    49081 agactgctgc gccatcaggc gcagtttcct cacacaccgt caggtgtgca gggagcgcag
    49141 gtcacggcgc gacaggggcg gcgcgaccgg aacggtcgct gcaagccggg ttttcacgca
    49201 tcggaccgta gcggcagccc gaaaccgcgc cagcgggttc ggcggagact ggcgggtgcg
    49261 ccagcagctg cctgtcggag tcgggcctga c
//
LOCUS       DAZGCS010000024        47668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGCS010000024 DAZGCS010000000
VERSION     DAZGCS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.024"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..1813
                     /gene="tssF"
                     /locus_tag="ACHK01_001467"
     CDS             53..1813
                     /gene="tssF"
                     /locus_tag="ACHK01_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8096440.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGSLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPFYLNADSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            1777..2856
                     /gene="tssG"
                     /locus_tag="ACHK01_001468"
     CDS             1777..2856
                     /gene="tssG"
                     /locus_tag="ACHK01_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8096441.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQFLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPNVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            2837..3373
                     /gene="tssJ"
                     /locus_tag="ACHK01_001469"
     CDS             2837..3373
                     /gene="tssJ"
                     /locus_tag="ACHK01_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8096442.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            3377..3805
                     /gene="tssE"
                     /locus_tag="ACHK01_001470"
     CDS             3377..3805
                     /gene="tssE"
                     /locus_tag="ACHK01_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8096443.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMVAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     YLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            3805..5181
                     /locus_tag="ACHK01_001471"
     CDS             3805..5181
                     /locus_tag="ACHK01_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO8096444.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     RVEKLSLALFRQNGVELQTQVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMKQPEKSEESPQPVFATRPAQQNDK
                     DASPSAPSPEISWQRTWPIFMAGMVVMACLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            5246..5794
                     /locus_tag="ACHK01_001472"
     CDS             5246..5794
                     /locus_tag="ACHK01_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HFO8096445.1"
                     /translation="MYKKERNRPFETREEVLAYHKREKIPCLECGKLLIFLPRHIWFV
                     HGIRADEYRVKWNIPKHIALAGISYLEKQSEYMKARIAKGKLDPAEQIAMMRIKGRQL
                     KSDPDWRNRSGSILKKQYARRDILKNRVWERSPSIKSVSVELKTEAIRRMKNRKVVGE
                     KVQDIADDLNVSISRLYHWLKM"
     gene            complement(6140..7393)
                     /gene="amiC"
                     /locus_tag="ACHK01_001473"
     CDS             complement(6140..7393)
                     /gene="amiC"
                     /locus_tag="ACHK01_001473"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO8096446.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7625..8956
                     /gene="argA"
                     /locus_tag="ACHK01_001474"
     CDS             7625..8956
                     /gene="argA"
                     /locus_tag="ACHK01_001474"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO8096447.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(9018..10844)
                     /gene="recD"
                     /locus_tag="ACHK01_001475"
     CDS             complement(9018..10844)
                     /gene="recD"
                     /locus_tag="ACHK01_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO8096448.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(10844..14386)
                     /gene="recB"
                     /locus_tag="ACHK01_001476"
     CDS             complement(10844..14386)
                     /gene="recB"
                     /locus_tag="ACHK01_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO8096449.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(14379..17267)
                     /gene="ptrA"
                     /locus_tag="ACHK01_001477"
     CDS             complement(14379..17267)
                     /gene="ptrA"
                     /locus_tag="ACHK01_001477"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO8096450.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(17443..20811)
                     /gene="recC"
                     /locus_tag="ACHK01_001478"
     CDS             complement(17443..20811)
                     /gene="recC"
                     /locus_tag="ACHK01_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO8096451.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCHEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(20824..21147)
                     /gene="ppdC"
                     /locus_tag="ACHK01_001479"
     CDS             complement(20824..21147)
                     /gene="ppdC"
                     /locus_tag="ACHK01_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO8096452.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(21132..21539)
                     /gene="ygdB"
                     /locus_tag="ACHK01_001480"
     CDS             complement(21132..21539)
                     /gene="ygdB"
                     /locus_tag="ACHK01_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO8096453.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVINGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(21536..22099)
                     /gene="ppdB"
                     /locus_tag="ACHK01_001481"
     CDS             complement(21536..22099)
                     /gene="ppdB"
                     /locus_tag="ACHK01_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8096454.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(22090..22560)
                     /gene="ppdA"
                     /locus_tag="ACHK01_001482"
     CDS             complement(22090..22560)
                     /gene="ppdA"
                     /locus_tag="ACHK01_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8096455.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(22745..23539)
                     /gene="thyA"
                     /locus_tag="ACHK01_001483"
     CDS             complement(22745..23539)
                     /gene="thyA"
                     /locus_tag="ACHK01_001483"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO8096456.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(23546..24421)
                     /gene="lgt"
                     /locus_tag="ACHK01_001484"
     CDS             complement(23546..24421)
                     /gene="lgt"
                     /locus_tag="ACHK01_001484"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO8096457.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24572..26818)
                     /gene="ptsP"
                     /locus_tag="ACHK01_001485"
     CDS             complement(24572..26818)
                     /gene="ptsP"
                     /locus_tag="ACHK01_001485"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8096458.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(26831..27361)
                     /gene="rppH"
                     /locus_tag="ACHK01_001486"
     CDS             complement(26831..27361)
                     /gene="rppH"
                     /locus_tag="ACHK01_001486"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO8096459.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(27714..27860)
                     /gene="ygdT"
                     /locus_tag="ACHK01_001487"
     CDS             complement(27714..27860)
                     /gene="ygdT"
                     /locus_tag="ACHK01_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO8096460.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            28046..28735
                     /gene="mutH"
                     /locus_tag="ACHK01_001488"
     CDS             28046..28735
                     /gene="mutH"
                     /locus_tag="ACHK01_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO8096461.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDIIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            28804..29517
                     /gene="ygdQ"
                     /locus_tag="ACHK01_001489"
     CDS             28804..29517
                     /gene="ygdQ"
                     /locus_tag="ACHK01_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8096462.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            29655..29873
                     /gene="ygdR"
                     /locus_tag="ACHK01_001490"
     CDS             29655..29873
                     /gene="ygdR"
                     /locus_tag="ACHK01_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO8096463.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            29981..31021
                     /gene="tas"
                     /locus_tag="ACHK01_001491"
     CDS             29981..31021
                     /gene="tas"
                     /locus_tag="ACHK01_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO8096464.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(31053..32246)
                     /gene="lplT"
                     /locus_tag="ACHK01_001492"
     CDS             complement(31053..32246)
                     /gene="lplT"
                     /locus_tag="ACHK01_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO8096465.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(32239..34398)
                     /gene="aas"
                     /locus_tag="ACHK01_001493"
     CDS             complement(32239..34398)
                     /gene="aas"
                     /locus_tag="ACHK01_001493"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO8096466.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            34984..36015
                     /gene="galR"
                     /locus_tag="ACHK01_001494"
     CDS             34984..36015
                     /gene="galR"
                     /locus_tag="ACHK01_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO8096467.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(36022..37284)
                     /gene="lysA"
                     /locus_tag="ACHK01_001495"
     CDS             complement(36022..37284)
                     /gene="lysA"
                     /locus_tag="ACHK01_001495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO8096468.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGLVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            37406..38341
                     /gene="lysR"
                     /locus_tag="ACHK01_001496"
     CDS             37406..38341
                     /gene="lysR"
                     /locus_tag="ACHK01_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO8096469.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(38328..39020)
                     /gene="ygeA"
                     /locus_tag="ACHK01_001497"
     CDS             complement(38328..39020)
                     /gene="ygeA"
                     /locus_tag="ACHK01_001497"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO8096470.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIDECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(39149..40567)
                     /gene="araE"
                     /locus_tag="ACHK01_001498"
     CDS             complement(39149..40567)
                     /gene="araE"
                     /locus_tag="ACHK01_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO8096471.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(40882..41643)
                     /gene="kduD"
                     /locus_tag="ACHK01_001499"
     CDS             complement(40882..41643)
                     /gene="kduD"
                     /locus_tag="ACHK01_001499"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO8096472.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(41673..42509)
                     /gene="kduI"
                     /locus_tag="ACHK01_001500"
     CDS             complement(41673..42509)
                     /gene="kduI"
                     /locus_tag="ACHK01_001500"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO8096473.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(42796..43977)
                     /gene="yqeF"
                     /locus_tag="ACHK01_001501"
     CDS             complement(42796..43977)
                     /gene="yqeF"
                     /locus_tag="ACHK01_001501"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8096474.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            44232..45461
                     /gene="yqeG"
                     /locus_tag="ACHK01_001502"
     CDS             44232..45461
                     /gene="yqeG"
                     /locus_tag="ACHK01_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8096475.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            45921..46553
                     /gene="yqeH"
                     /locus_tag="ACHK01_001503"
     CDS             45921..46553
                     /gene="yqeH"
                     /locus_tag="ACHK01_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8096476.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            46887..>47668
                     /gene="yqeI"
                     /locus_tag="ACHK01_001504"
     CDS             46887..>47668
                     /gene="yqeI"
                     /locus_tag="ACHK01_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8096477.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDTNIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL"
BASE COUNT        11886 a        12721 c        11696 g        11365 t
ORIGIN      
        1 atctcactac tcgttggtat taatttcaat aaaaggaaag ataaatcctc cgatggacga
       61 cttaacccaa cgctactacg aagccgaaat gcgctacctg cgtgaagcgg ggaaagaatt
      121 tgcgcaggct taccccgacc gcgccgcgat gctcaatctt gataaacccg gcgcacgcga
      181 tccctatgtg gaacgcctgt tcgaaggctt tgctttttta atgggcagcc tgcgcgagaa
      241 gctggacgac gatctgccgg agctgactga agggctggtc agcctgctgt ggcctcacta
      301 cctgcgaact atcccgtcac tttccgtggt tgaactgtcg acagatcacc ggcagatgaa
      361 gcagagcgaa acgctgagcg attttcaggt attgtcgcgc cctgttggcg agcgcagaac
      421 ccgctgtttc tacagcgcca cgcgggatat cacgctgcat ccgctggcgt tgccggacgt
      481 gtcattgcaa tatgaaccgg acggacgttc ggttattcgt ctgcgctttg aatgcggccc
      541 gctggtcggt gactggtcac agatcgattt atcccgcctg ccgttctatc tcaatgccga
      601 cagtccggtg gcctgtgcgc tgcaccgggc tttaacgctt ggtacgcagc agttctggtt
      661 gcgtctgccg gggcaggatc gccggacgct tgacgcacac ttttccccgt tgggctttga
      721 ggataatgac aggctctggc caaaaggcga gtcggcgttc agcggctacc agttgctgct
      781 ggagtatttc accttccgcg agaagtttat gtttgtggca ctcaacgggc ttgagctggt
      841 tgcatggccg gaggggataa ccggctttga gattgacgtg gtgctgaacg agaactggcc
      901 tcacgacctg ccgtttgaca gcgataatat ccgtctgcac tgcgtgccgg tcatcaatct
      961 gttcccgctc gaagccgatc cgttacacct ttcgccactg gaaaacgaat ttctgctacg
     1021 cccgatgcgc attcaggacg ggcataccga gatttattcc gtcgacaaca ttatttcttc
     1081 gcgccatacc ggcagtcagg cgtatgtccc cttctccagc tttcgtcacc ggggcgggat
     1141 gctgcgccac gatgcgccgg aacgctatta ccacacgcgg gtaaagcgcg gcccttccgg
     1201 tctgcatgac acctggctga ttctgggcgg tgatgcgttt gatgctgacc ggatgctgga
     1261 ggatgaaacg ctctctttaa gcctgaccgg tactaacggg catcttcccc gtaaggcttt
     1321 acagagcacg ctgctcgata ggccagttca tgcctcacag aacgtcctgc gggtgcgcaa
     1381 cctgtgcgcc ccaacgcagc cctgctatcc cccggcgcgt gaccgcttcc actggcgggt
     1441 gctcagccac cttggttcta actttttatc gatgatggat aacgccgaaa ttctgcgcgg
     1501 aacgctggcg ctgtacgact ggaccgaaag cgagatgaac cgccgtcgtc tggaggcgat
     1561 tgtcgacgtt cagcacagcc tgatacagcg ttttgaaaga ggctttctgc tgcgtggcgt
     1621 ggatattcag gtgacgctgg acagtaacgg ctttgccgga gaaggtgata tcacgctgtt
     1681 tggtgaactg ctgcaccgct ttttcgcact gtatgccgat atccacttat tcactcaact
     1741 gaccttaatt ctgcaaccta caggaaagtg cctgcaatgg acagagcatc acagccagcg
     1801 cgtaccgggc tgaccgaccg gctggccccg gatattgcgc ggatcaattt ttaccgtttt
     1861 tgccagtttc tggaacagag ccagcagaac gcgccgctgg gcagtaccga caaccccgcc
     1921 accgatgcgg tgcgctttcg tccccatccc ggaatgggct ttccggtcag cgagttaaag
     1981 aacgttgaac gtgacgcggg caatcccaac gtgccaccca cggtgcgcac cacctttctc
     2041 ggcctgtacg gcgtggattc gccgctgcca accgcctatc tggactacat tacccagcgt
     2101 catgacggac atgacgcagt gatggcgttt ctcgatattt ttaaccatcg ctttatcacc
     2161 cagtattacc gcatctggcg caaatataac tacccggcgt cgtttgaagc aggtgctatg
     2221 gatgacattt cccgttgtct gctgggactc atcgggctgg gtattcccgg cagcgagaac
     2281 catatcgcca cgccggtatc ccgttttctg gccctgctca gcgtgatgcg cctgccgacg
     2341 cgcacggcgg aaggcgtcac ggcactggtg ggactgcttg ccccgctgac gaaagcgacg
     2401 gtggtgcctc acgatccgca gcctgttatt ctcccggccc ctgcgggtct gtcaaaaaat
     2461 tcacgcatca gcctgaaaac gcgaacgctg ctggggcgta ccggcaccga cgtcaacagc
     2521 cagttgctgc tgaaactgta taccgaagat gccgcagaag cacgtggctg gctacctggc
     2581 gggcagctgc acagcgattt gctggtgctg ctgcgcgttt atctgggctg gcgctgtcag
     2641 gcccgtctgc aactgacgct gcccgtcagc ctgttgcctg ctgcccggct gggaaaacag
     2701 cgcgtacaga ttagccgtac cggcatcctt cgcgcctcct ttgccgcgcc cgcgaccgga
     2761 acagtgacgg tcagtctggg gcgttatcag ggattaatac ccgctttatc cattcgtaac
     2821 agggagagta tgactcatgt ttcctattcg ttttaaacgt ccggcgctgc tctgtatggc
     2881 gatgctgacg gttgttctga gcggctgcgg gctgattcag aaagtggtgg atgaatcgaa
     2941 aagcgtggcc tcagccgttt tctacaagca aatcaaaata ctgcatctcg atttcttctc
     3001 ccgaagtgcc ctgaatacgg atgcggaaga tacgccgctt tccacgatgg tgcatgtctg
     3061 gcaactgaaa acccgcgaag attttgacaa ggcggattat gacaccctgt ttatgcagga
     3121 agagaagacg ctggagaagg acgtactggc aaaacacacc gtctgggtaa aaccggaagg
     3181 cacggcatcc ctgaatgtgc cgctggataa agagacgcag tttgtcgcca ttattgggca
     3241 gttttatcac cctgatgaaa aaagcgacag ctggcgtctg gtgatcaaaa gggacgaact
     3301 ggaggccgac aagccgcgct cgattgaact gatgagaagc gacctgcgac tgctgcctct
     3361 caaggataaa taaccgatgt cgacaccttc cttatatgaa atgctgacgt tcagtttcag
     3421 cggcgagtta ccactggaac aggtcagcga gcgggatcag cttatccttt ccgtgatgga
     3481 caatatgcaa cgcattatca actgtcgggc aggcacactg gcgcatttgc cggattacgg
     3541 tctgccggat ctcagtctga tccatcaggg tatggttgcg ggtattcatg gcctgatgcg
     3601 tcagattgaa gaaacgctgc tgcgttatga accacgcctg agtcagatac aggtggagtt
     3661 actcccccag ccccgtccgg ggtatcttaa ttacctgatc cacgcgcagc ttcccgatac
     3721 cggctggata cgctttgatg gcgtgttttc tccggaagga cgaattgttc tgcgtcatct
     3781 caaacagcag gagcgggcgt actgatggca agtaacgcga attttatcag ccagttcgtc
     3841 atgggcggcg atccctgtac ttataaggaa tccggtgaac tacaggctga aatgagtaaa
     3901 ctgactcacc cggcccgacc tgatgtggac tggcgtcggg tggaaaaact cagcctcgcg
     3961 ctgttccggc aaaatggcgt ggaattacag acgcaggtct gttacgtact ggcgataacc
     4021 agacggcagg ggctggcagg gatggcagac ggactcggtt cactggacat actgctccag
     4081 cgctgggctg acttctggcc ggtacaggta cattccagaa tatcactgct cagttgggtc
     4141 acagaaaaaa tgcagcaggc actgagaacg ctggatattc agtatcagga tctgccgcag
     4201 atttaccgtt gtgtacagca tctttccgcc atcgaaacca cgctgcaaca gtgtgaactg
     4261 tggcatatga cgaaactgga tgtgctggcc gggcagtttc gtaatacggc gctacgtctg
     4321 gagcggctgg caccacaggg agcggaaacc actatcactc cccctgaatt accccgccgg
     4381 gaaatgaagc aaccagaaaa gtcagaagaa agtccacagc cggtttttgc aaccagaccc
     4441 gctcagcaaa acgataagga tgccagtcca tccgcgccat cccctgaaat ctcctggcag
     4501 cggacatggc cgatatttat ggccggaatg gttgtgatgg cctgtctcgg cggaacagga
     4561 ttatggggct ggtcgcagct taatcagccg gacgcactaa tccagcgaat acaactgtct
     4621 gtcatgccat taccgcagtc gctggagagc ggcgagctgg caaagctgga tgtaaaggat
     4681 aaggcgctgc tggctcagga cagaacaatt gcggcaagtc agatgcaact ggagcagtta
     4741 aacaaattgc ctgcccgctg gccactggag cagggatatc gccagctacg ccagcttgat
     4801 gccctgtggc cggataatcc tcaggtcaga gcgctgaacg cgcagtggcg taaacagcgg
     4861 gagctgagcg ccctgtccac agaggcattg aatggctacg cacaggcgca gagccagcta
     4921 cagcgcctgt cggcgcagct ggatgcactg gatgagcgta aggggagata tctgaccggt
     4981 tcggaactaa aaacggcggt gtacggcatc cggcagtcgt taaaggagcc gccgctggaa
     5041 gaactgcttc ggcaactgga agagcaaaaa cagaccggag aggtttcgcc gacgctgttg
     5101 acgcaaattg atacccggtt aaatcagttg ctgaatcgct atgtaatttt actggatacg
     5161 aaggtggaac aaagtcagta aacgtcgggg gatttatgca ccaataaggg atatgtgacc
     5221 ttgccatttt ttaaaaggag tctttgtgta caaaaaagag aggaacaggc cattcgaaac
     5281 gcgtgaagag gttctggcat atcataagcg ggaaaaaata ccctgtctgg agtgtggcaa
     5341 gttgctcatt tttttaccgc gccatatctg gtttgtgcat ggaatacgcg ctgacgaata
     5401 tcgtgtaaaa tggaacatac cgaagcacat cgcactggca gggatttcat atctggagaa
     5461 acaaagtgaa tatatgaagg cgagaatagc aaagggaaag ctggatcccg cagaacaaat
     5521 agccatgatg agaataaagg gaaggcaact aaagtcagat cctgactggc gtaatcgctc
     5581 cggttctata ttgaagaaac aatatgcccg acgagatatc cttaaaaaca gggtatggga
     5641 aagatctccc agcataaaaa gtgtctctgt ggagttgaaa acagaagcta taaggcgaat
     5701 gaaaaacagg aaggtcgtcg gggaaaaagt ccaggatatt gccgatgacc tgaacgtatc
     5761 tatcagcaga ttgtatcact ggctaaagat gtaggcgctc atgtgctctc aatgccttca
     5821 ataaagccgc tatgcgcgaa ccggcacatc tggtagactg ttctcagtga gtcaagcaaa
     5881 ctgcctactt ttctaacttt taatcatgaa taaccagcgc cttgagtata gctggttcga
     5941 atatactcgt tgattcacat cagataatga gtgtataatt cggtagatag cttgggttcg
     6001 ggaacagtga atgccttaag tttcaaaagg ttagattaac ctcttgactg tggcccccgc
     6061 aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgctctccca taaaaaaagc
     6121 gccattcagc gcctttttat catccccttc tcgccagcgt cgccccatcg gcaaaatacg
     6181 ctttaattcc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc gttttcagtt
     6241 tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg
     6301 gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc ttgttgattt
     6361 taccgagctt attcagtacc gctttaccaa acttcaggct gtcggcaatg gtcagcgact
     6421 gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc acgccaccaa
     6481 tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcacctt
     6541 tggttgagag cgcaaacaca gaggaaccgc tcggctgacg actggtaaag gcgtcggcat
     6601 ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc acttgcaacg
     6661 gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca
     6721 gagagcgcaa acggcgagct atttgcaata ccacgtcttt ttcgcgcgtt ttgtatttcc
     6781 ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat
     6841 cgcgccctgc tttacctggt tgtggaccac tttgcgctgg cggcacctgc ttttcgaggt
     6901 cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg
     6961 ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca
     7021 gctgcggttt tacgttttgc tttaattcaa aaaccatccg tacggtttgc gggtcaaatt
     7081 gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc gccatcccct
     7141 tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttcc gggttactca
     7201 acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacacgg gtgtagctgg
     7261 acgcaggcca gacgcgcacc gccacgacct ggctgaccgc agccagactg acctgactta
     7321 cgctcaatag ccacatggca cccgcgcctt gcagtaaacg acgacggctg attgcagtgt
     7381 tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac
     7441 taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa acggtaaaca
     7501 ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa atttacactt
     7561 gcaccctgcg aaaaaacaga ataaaaatac actaatttcg aataatcatg caaagaggtg
     7621 tgccgtggta aaggaacgta aaaccgagtt ggtcgaggga ttccgccatt cggttcccta
     7681 tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca
     7741 tgagaatttc tccagtatcg ttaatgatat cgggttattg cacagcctcg gcatccgtct
     7801 ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc atcaccacga
     7861 accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca
     7921 ggctgcggga acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac
     7981 gccgctgcag ggcgcgcata tcaacgtcgt cagtggcaat tttattattg cccagccgct
     8041 gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga ttgatgaaga
     8101 cgcgatccat cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc
     8161 agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac tagccatcaa
     8221 actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta atgacgacgg
     8281 tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag cccaggaaga
     8341 gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg
     8401 cagcggcgtg cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga
     8461 gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgctg agcagattcg
     8521 tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc tggagcagca
     8581 aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat
     8641 tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa
     8701 gattggggaa atggcctgtg tggcagttca cccggattac cgcagctcat cacggggcga
     8761 ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt
     8821 gctgaccacg cgcagtattc actggttcca ggaacgtgga tttaccccgg tggatattga
     8881 tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat
     8941 ggcggattta gggtaagcga tgaaaattgt cggatgcgac atgcgtaaca ctcgtgcgtc
     9001 gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc actgcgccgc
     9061 tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag cgacagacgg
     9121 cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg cgtgcgttgg
     9181 ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc
     9241 gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat attgccgtcc
     9301 ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca
     9361 ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcgacc ttcgtaccaa
     9421 cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca
     9481 ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagttg gtactcattg
     9541 aacgcctgaa tgattaaatc cggctcagca cgcgcttgca gcagatccag ataacgtcca
     9601 taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg taaaagtcgt
     9661 ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg
     9721 ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc
     9781 agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc cggaacgtga
     9841 gtccccgtca ggcggcttag ctgcccggca cgctcggcgg taaagccggc attggcataa
     9901 gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc acgatcgccg
     9961 agaaagatca ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat cataggcaga
    10021 tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt accggcatga
    10081 tgacgtaaac gctggctacc cggctgcgca cccagcaatc ggtgcaaagt actggcatct
    10141 tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga
    10201 gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt
    10261 tcgccgtcgg ccatttgaat taacgctgcc agtaacttcg ccacggtggt cgttttaccg
    10321 gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc cgctgcaact
    10381 ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg cgccagtaga
    10441 gcttcatcaa cctcaatggc atgattcacc tcattgaaga agcgcgctac cgtccgctcg
    10501 ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg cgtgggttca
    10561 tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg tagctcaccg
    10621 atttcactga cacaggtcgc caacagcgga tgcgacgcct cgttattttc cagtcgtgaa
    10681 agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc cgcgagggtg
    10741 acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag cgggcgtagc
    10801 tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccaggg
    10861 tcataccggc aaacatctca tccatcaggt caatcaaccc ggcgttgggt cgggttgtgt
    10921 aaatcccttg ttgcggatgt tctttatcaa caccacgcag gaacagataa ataacgccgc
    10981 caaagtggcg ctcatagtcg taatcagcaa tgcgatggcg cagataacga tgcagcgcca
    11041 gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgccgct gccatagcct
    11101 gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg agcaggtaat
    11161 aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg cgtacctgca
    11221 tgaactccag cggcgggcag ccagcggata gcggatcaaa ctggcgaatt agtgcatcaa
    11281 gctggctggc gatgagcggt tcactaatcg gcaggtaaaa ctccatctcc acctgtttat
    11341 cgcggtcgga aagctgattc aggctaacac ccgtttcatt gagaggtgcc tggaggacag
    11401 ccgtgatcca ctcggtcaac actggttccc actgcgattc aaagccgccg agttccagtt
    11461 tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca aacaaactgt
    11521 gcaagaatgt ccctggtgac gcaccgcgcg ggaactgatg cggtgttaac gtcggttctt
    11581 caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga tcctgagcga
    11641 taccgtgacc acgctgctgc aaaccggagt agctggtgac acgccagtta tcgccgggca
    11701 atcgttgtag cgtcctcgca ttcagttctg cagttaaagc atcattaacc tgccagggct
    11761 gtttatcacc cgtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac
    11821 aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac
    11881 tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc
    11941 cgagactgca atgccaaacc gaacgtgtca gcgccacgta aagcaaacgc agatcttccg
    12001 ccagacgttc ggcctccgcg aggtcgacgc tttctggcgc agcattaaga tccagaactg
    12061 cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa ttggtgataa
    12121 acggcagcca gaccaatgga tattccagcc ctttcgattt gtggatcgtg acaatctgca
    12181 ccagatgttt atcactttcg agacgcattt gttggctgga ggcattactg tctggctcga
    12241 ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt
    12301 cttgtagcag ttcgctgata tgcaagatat cggtaagtcg ccgctcaccg cctgccgttg
    12361 ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc
    12421 cacgtttgcg ccagatttgc cgataaccat cgaactcttc gaccaccgcg tcccacgcat
    12481 gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt gacgttgcca
    12541 gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac aaccaaagca
    12601 tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa acggaaggga
    12661 tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca
    12721 cactgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc
    12781 cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc gccatggtac
    12841 tttgataatc gccaacgccg cagctttcgc cttccatcag ccacattttc atcgcaggct
    12901 gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccgactgat ttcactggaa
    12961 taaacggtat ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc ttattcacgc
    13021 tgttcaccat tcctggtgcg gaacgccagt tggtgtctaa agtgtagtgg gcgtgaactt
    13081 cgctacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat atggcctgct
    13141 tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata cggcgaaaaa
    13201 ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg
    13261 tacggatcgc cgctgccaac acctcaccgc tttcgctacg cagcgcggaa tcgagccgac
    13321 ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc
    13381 ggatctcagc caatgcgcgg gtgatcacca gatcgcggat cgacaatggt tctgcaagca
    13441 gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggct ttcgtgcgat
    13501 cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa ctgttggtct
    13561 cttcttctgc ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact
    13621 tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc
    13681 actgctgttt taccgtagca atacgcgcca caatttgcgc gtggcgggaa gccagcgttt
    13741 catcatcggg cggcggtgct ttgataaccg gcgcttcgcc ttgcagataa cgattaatat
    13801 cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct atttcacgcg
    13861 gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtaacgt agcagagact
    13921 catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca
    13981 tgcgctggca aaaaccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta
    14041 acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcaga cgttcgtaca
    14101 gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcatggata ttgctacgga
    14161 tacgaccacg caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa
    14221 cggtcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg cgcaaataga
    14281 gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcaccct
    14341 gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc
    14401 aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcagggtgt
    14461 acatattcgg ctttcccatt ctggctgccg gaaatctgcg acaggatagc catgccttgc
    14521 ggctcgacta ccgcctgatg gaagaaatcg gccagttttt gcggcgtcag cagtttaatc
    14581 tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt
    14641 aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc
    14701 tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcagttggg
    14761 aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg
    14821 aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat
    14881 tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag
    14941 ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg
    15001 gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca
    15061 tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc
    15121 agcgttgttg cctgggcttc ggtcatgttg ccaataacca taaactctgg tcgagcccct
    15181 gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg caaaattttg
    15241 cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc
    15301 tgctcgaacg ctttgccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc
    15361 tgctcaagct gatcttccgt ggcggtatag ctaaagtagc cttccagcaa tgcctggaac
    15421 agctgcggca gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg
    15481 gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct
    15541 gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga
    15601 ttacgcaaaa tcaggctgac atcggctttt ggctcgctgg caaaataacg gcttggcgca
    15661 tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcata tttcttgtct
    15721 gacttaatca gcgagaaatc gtcaggaata taagggttaa gctctggcaa agagagcgca
    15781 atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt atcgacctga
    15841 tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct gatataccag
    15901 atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt agcatcgtac
    15961 cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat catggtatct
    16021 gccagccatt cgacgtaatc catatcacgg gtgattgacg gataacggaa gtcgatatcc
    16081 agcacattcg ccagttcatc gaagtattgt ttatcgatcc ctttttcacg taacaaattg
    16141 aggtagctaa aaatagccgc cacaacctga tcgcgattcg ccagaccttt atcggttaaa
    16201 gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta
    16261 atgccctcaa ctaatccctg cttttgcagc cagtcggaaa gtgtacctgg gctgcgatta
    16321 ccaatcagat aggtaatcaa ttcatcggtt ttgctacgga atttcgccga gttgttatca
    16381 atacgaaact ctacgcgcaa cactttacgc ggcagcgccg ggacgtaatg aatgataatg
    16441 cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc
    16501 ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg tttattgctg
    16561 taaataaccg ccttcatcag attggcggaa tagtacttct cgtggaaatc tttcagcgcc
    16621 tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc agaaaacttt
    16681 gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca
    16741 cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc ggcgtatttc
    16801 ttgtcgagca agggttctgc aatagcatca gccaggcggt ctaccgcacc aggcaaggcg
    16861 tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta
    16921 ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc
    16981 atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc
    17041 gacccaacgg gcaccaccag cgccgagagc gatttaactg cctgcggatc agaaaccagc
    17101 aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt
    17161 ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa
    17221 agggcaacta acaacaataa tgctttgaac caggtgctgc ggggcattca cggacctcat
    17281 aatcaactta attttctgtc cagattcaac acgttaacgc aaatcatctg ccagaattta
    17341 atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga ctggtgcgca
    17401 tcataaagca agcggataga ttgcgcaatt tttatacagc actcatgact gattaaagcg
    17461 aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg tctctggtgt
    17521 taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca ccatcatgtt
    17581 gccttcgtaa gcctgcagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag
    17641 catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa
    17701 caccagcaat ggcgcggaca tcccttcacg atacccctca atcagttgtg agaggtaatg
    17761 caaagcctgt tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag
    17821 gcgactttca ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat
    17881 tccctgcgcc acacttaata aagagggacg ccagcgcaac aagccatccg gctgcacctg
    17941 cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc
    18001 cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcatggc actgtgtttc
    18061 ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcggcggaa
    18121 caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata
    18181 acgactaagc ccttcaagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg
    18241 gaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg
    18301 tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga
    18361 atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc
    18421 gcctggctgg tagttttgtg gatcaaacgg catccgggta tggaggcaag taagatgcgc
    18481 ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact
    18541 ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc
    18601 ctgaatggag cgcccgatat agctgatata aagtttttgt tgcgcggaaa ttaacgcttc
    18661 caggaacaga tagcggtcat catcgcgacg gctacggtcg ccacgcttcg gtttctggct
    18721 catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag
    18781 caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg
    18841 tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgcgcca gttcatcacg
    18901 caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc
    18961 ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa
    19021 ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct cctgtgccag
    19081 cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc
    19141 aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc
    19201 catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc
    19261 ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt
    19321 gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac
    19381 cggcacatcc agtaatgcca gcacgtcttc cgacacaaag cggctgtctg gcaacgataa
    19441 caggctgata aacgcttcca gtaccggatg cgactgccgc gcccgacggt cggaaatggc
    19501 gtaaggcagg taacgatccg caggtgcgct accaaacaca gcctgaataa acggactgta
    19561 gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcttc
    19621 cagcatcgcc agcaggcgat cgtgcaaaac ttcaacttca cgctgcgggc tatggcaaac
    19681 gtggaaggtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa
    19741 ctcttcgatg ttcacaccag caacggcacg gttttccagt tccagaatgt cagactgaat
    19801 gttatgcagc aggttatctg gcgtgacatc gacaaaggcg tccagctcct ggctgctctc
    19861 caggtcagaa aggagataaa tgtagtcgcg cccaagctta ccccatgaag ccagcagcgg
    19921 gttgccgaca tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctatcacg
    19981 aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca gcagtttcgc
    20041 cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg taaacaggag
    20101 atggatttca atatgtttac ccagcgcctg tagcgcctgg agataaacag gcggtaacgc
    20161 ggaaataccg catataaaga cgcgcgaagg tagccccggc gggcaggtcg tcgcggactc
    20221 cagcgtttcg ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gcccgagttg
    20281 atgggtatat tccaccaacg ccttccacaa cggcgcttgc caggcctgtg cttctcccag
    20341 cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat agaccagata
    20401 ctggtcaaac aggtccgccg cttttgaaga aagctggaac agttttcgct tgtcgctatc
    20461 gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccaacaatt gcggcagcag
    20521 agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg
    20581 taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc
    20641 cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac cggtactttg
    20701 caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc
    20761 catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc
    20821 tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga
    20881 aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg
    20941 ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat gccgccagag
    21001 ctgttggtac tggtttctgc tggcgagact gttcattaat gttcgctgga tacccgataa
    21061 cgcagtgaca atcatcacca tcagcaccat cgcaaccatt acctccggca ggctaaagcc
    21121 ttgttgattc ttcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc
    21181 cagccgcgtg gcgaaaaaac aatgtttcca ttaatgactt cgcctgttcg ccacaacgaa
    21241 acgccttcat aaccggcaat caataaggct tcattatctg ccagtaaacg caaacaaacc
    21301 tgggcatcgg ttccagcgta ctgcgagcac tgaactgctg gctgcgtctg ccaggagtgc
    21361 atttttcccc acgccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttcc
    21421 atgctcacgc gagaggcaaa actgcgatcc tgctgactca ttccttgcaa tagcaagcta
    21481 cccaaaacca gcagcatcag gaccagagcc agtgacgaaa cacctttttc gcggttcaca
    21541 ggttgaatcc tgtcacgcta tagctggcat ccaccacggt ttgcggttca gacttactgg
    21601 cagcacgcat attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct
    21661 gaaaagtgtc gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac
    21721 aggatgtcgc accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt
    21781 cagactcttt taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa
    21841 tgatacagtc accctgtccg acaatttcca gcccttcgcc ggtacagctg ccatgacaat
    21901 aacccgccct ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca
    21961 gcgccagctt acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgcag
    22021 cccccagcaa caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac
    22081 cttgctcttt tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca
    22141 cggcgaaacc accagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca
    22201 tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc
    22261 gggccagcgt ggcacaaaga ccaatggtga actgccatga caggtattgg ccccagcagc
    22321 ggaactcaca aggcaccata acgtcccctc cctgataacg ctgatactgt ggtcgcggtt
    22381 atgccagttg gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg
    22441 ccatagccgt tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat
    22501 gaccaaaatc agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat
    22561 gccggcagta tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca
    22621 tcttccggac tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg cgtctcgaag
    22681 aatttaacgg agggtaaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc
    22741 gtaattagat agccaccggc gctttaatgc ccggatgtgg atcgtagcct tcaatctcaa
    22801 agtcttcgaa acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca
    22861 gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatgattg ctgtacaggt
    22921 gcgtgtcgcc tcccgtccag acaaaatcgc ccacttccag atcacactgc tgcgccatca
    22981 tatgcaccag taacgcgtag ctggcaatgt taaacggcag gccgaggaaa acgtcacagg
    23041 agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaacgcat
    23101 ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc
    23161 ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg tcaatatgac
    23221 gaccatccgg cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccgt
    23281 tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag
    23341 tgtcgccctg cagaaaccac agcagttcat ggatgatgga acgcaggtgg caacgtttag
    23401 ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa
    23461 gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca
    23521 gttctaaata ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg
    23581 cccagaccat catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc
    23641 tgatgtactg cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa aactcaacaa
    23701 tgatgcgaaa cgcgccgtag ccaatcagga acaagccaga gacagctccc attgggcgcg
    23761 gtttacgaat atacaggttg aggataataa acagcaccac accttccagc agcagctcgt
    23821 aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg
    23881 ggttggtttg cagcagcaaa atatcttctg tacgggagcc tgggaacagc atggcaaacg
    23941 ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg
    24001 caccaagacc aaacggaatg agtggtgcga taaaatcaga aacctggaag aaggaacgtt
    24061 tagtacggcg ggcaaagata atcatcacca cgataacgcc aatcaggccg ccgtggaaag
    24121 acatgccgcc gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaaat
    24181 tgtagaacag aacataacca atacgtcccc cgaggaagac gccgaggaag cccgcataga
    24241 gtaagttttc aacttcattt ttggtccagc cgctgcccgg acgattcgcc cgtcgtgttg
    24301 ccagccacat tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca
    24361 cgggtcctat tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca
    24421 tctgtcacca caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg
    24481 caggtttcag gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg
    24541 ataccgttaa aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca
    24601 tgccgcgacg ctccataaag gctgcaacct gatggcgaac ttcggtcgcc agttgcgctt
    24661 ccagactacg ctgcgcaaga ttttctgctt cggcataatc aatgcgccgc agcaggtatt
    24721 ttacccgcgc tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga
    24781 ttgccacgca catgggatcg cccgccattt caccgcacaa acggagatcg attccatgta
    24841 tttccgcttc ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcat
    24901 aaatgttcgc cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt
    24961 tggtgccaac agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg
    25021 acggcacttc cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct
    25081 cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc atcaacttca tcgaggcttg
    25141 tgaccatcgg caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc
    25201 gcacctggat caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca
    25261 ggcatggatt ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca
    25321 gcgtacgcaa ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca
    25381 cctgttcttc ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa
    25441 gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccagcat
    25501 tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt
    25561 ccgccagacg gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta
    25621 ccggctccgg atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat
    25681 gcagcaccga aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca
    25741 tgatcgcagc atgggagttg gctgcgccat ctcgcacgac aacaccgact aagcgatcct
    25801 ggggcagctc agcaagagtt gtcgctgaca gttcatccgc caccagaatg aaacgttccg
    25861 gccaggcgtt cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg
    25921 cacgtaaatc gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg
    25981 caaatttttc aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg
    26041 caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg cgagtaaaga tcgaaaatag
    26101 ccgccgtttc tttttgtgca ccggcggcaa agcgtttgct gtagcggcga aactcgtttg
    26161 ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg
    26221 cctgatacac ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca
    26281 caccaggtgc tgccggtaat gcgcggattc gcgtctggcg atattgccca aacagggcag
    26341 tcaactgcga ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt
    26401 cttcactttc gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact
    26461 ggcgacgttg aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg
    26521 ggatgtattt gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc
    26581 tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt
    26641 ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga
    26701 ccgaacagac ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct
    26761 cattcaggcg tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa
    26821 ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc gttttgtggt tttggcgtat
    26881 tttcctgcag tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac
    26941 gtttaaatga caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa
    27001 actctggtgt actgctggtt tgcatattga tttctgcatc gccgctcacc agctgcaaga
    27061 gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt
    27121 tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc
    27181 ttaatcctac ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt
    27241 tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct
    27301 gcccctggcg attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca
    27361 ccggactacc tcaaaataaa gctttatata cgaatgattg tttcatactc caggaagacg
    27421 gtaaaccact ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt
    27481 gcagagttat aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata
    27541 gagttgtgaa gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga
    27601 tatagacatt ttaaaatatt cattattagt caatgcatta catgtaattt gagtaataaa
    27661 aaagagtaat tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca
    27721 atgccgtatt tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga
    27781 gtttcatcgc acgtcaaggc tgtaaatgga aacagtagtg gaggtttttc acagttatcc
    27841 cagctttctg tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa
    27901 aacctattgc agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcagg
    27961 gaattgaaca acacatgtgg aaaaagttac actgcgaata ttcggcacat aattgctgtt
    28021 tgttttttaa tcaaggtatc atgacatgtc ccaacctcgc ccactgctct ctcctcccga
    28081 aactgaagaa cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact
    28141 ggcggcactt gccgggcttg ttacgccaga gaatttaaaa cgcgataagg gctggattgg
    28201 cgtgttactg gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc
    28261 cgctctgggc gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac
    28321 aacattcgtt tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca
    28381 tgtgcgccac aagctcaaac gcgtactgtg gataccggtt gaaggcgagc gcagcatccc
    28441 gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg
    28501 acagctacgc gaagactggg aagaattaat ggatatcatt gttctcggtc aggttgagcg
    28561 gatcaccgct cgtcacgggg aatatttaca gataagaccg aaagcagcga atgcgaaagc
    28621 gcttaccgaa gccattggtg cccggggcga acggattctg acgctgccac gcggctttta
    28681 tttgaagaag aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc
    28741 gctttgacct gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt
    28801 tagatgttat ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacgctgacg
    28861 ctgctggaga tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag
    28921 cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg
    28981 cgtctggcgc tgctggcatc aatcgcctgg gttacgcgcc tgacaaatcc gctttttaca
    29041 atattcagtc aggaaatttc tgcccgtgat ttgattctgc ttctgggtgg cttgttcctt
    29101 atctggaaag ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa
    29161 acacgcgttt catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttt
    29221 agcctcgact cggtgattac ggctgtgggt ctgtcagatc acctgtttat tatgatggca
    29281 gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc
    29341 gaacgccatc cttcggtaaa aatgctggcg ctctctttcc tgattctggt gggctttacc
    29401 ctgattctgg aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc
    29461 ttctctattg cggttgaaag cctcaacttg attcgcaaca aaaagaatcc gctctgatac
    29521 tccgtacgct ctcctgcggg agggcgtact cactcctttt accctcacag tttaagattt
    29581 tcctgctttc aagatatata acgtcggttt ataaacagac tattatcata ggtaggccaa
    29641 atgaggataa ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg
    29701 ctgtttccgg gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga
    29761 ccgatggaaa acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag
    29821 gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa
    29881 ggtcagcatc cggctggcct taagattttt ctctttccct tttcccttcc ctctgccatt
    29941 tttatattcc ttatgtcgtg attataaaaa ggaaacggct atgcaatatc accgtatacc
    30001 ccacagttcg ctggaagtca gcacgctggg gcttggcacg atgacgtttg gtgaacagaa
    30061 cagcgaagcc gacgcccacg cacaactcga ctatgccgtc gctcagggca ttaaccttat
    30121 cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa acgcaagggt taaccgaaac
    30181 ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa aagttaatta tcgcctccaa
    30241 agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc ccggatcagg cgctggatcg
    30301 gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc ctacagactg attacctcga
    30361 tctttatcag gtgcactggc cgcagcgccc gaccaactgc ttcggcaaac tcggttatag
    30421 ctggacggat tctgcgcctg cggtttcgct gctggatacg ctggacgcac tggcagagta
    30481 ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac gaaactgcat ttggcgtaat
    30541 gcgctacctg catctggcgg acaaacacga tctgccgcgt attgtcacca ttcagaaccc
    30601 ttacagtctg ttaaaccgca gttttgaagt aggtctggca gaagtcagcc agtatgaagg
    30661 ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg ctgaccggga aatatctcaa
    30721 tggtgcaaaa cccgctggcg cacgtaatac gctctttagt cggttcaccc gctatagcgg
    30781 tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc gccagacgtc atggcctgga
    30841 ccctgctcag atggcgctcg cgtttgtacg ccgtcaaccg tttgttgcca gcactctgct
    30901 gggcgcaacc acgatggatc agctgaaaac taacatcgaa agtttgcatc tggagttaag
    30961 cgaagacgta ttagctgaaa ttgaagcggt gcatcaggtt tatacttatc cggcaccata
    31021 agattaacgc cggttaaaac cggcgttaaa tattaatgac ggcgctgcca gatccacagc
    31081 gccgttattg ccagcgcaaa cagcgcaccg aagccaatgc caatgggcac gaccgggatg
    31141 cctatcatta ccgccagcga gtaaatgccc agcatcaaca acatggcgct gttttcgcca
    31201 aggttttgta ctgcaatcgc attccccgcc ccgacgcttt ttttaccccg ctcctgtagc
    31261 aacgcattga gcggaacgac aaaaaagccc cccatcacgc caatcagcat caacaaggca
    31321 taggctggca gcagctcgtg ttgcagggaa aaaatcagta ccaccacgcc aatcaaaatc
    31381 ccggctggca tacagcgtga cacggtttcc agcgtaacta acttcgctgc cgcacctgcg
    31441 ccaaccacga taccaatcgc taccatcgcg ttgagatagg tgggcgtagc gttatcggta
    31501 atgcccagcg ccaccggtac ccacagcacc aacaggaaac gcagcgtgac acccgctccc
    31561 cagaataaac tggtgcccac cagcgaaaaa cgcgtttcac cattgcgcca tagcgaggtg
    31621 caggcattca ggaaactgcg ggtcatgttg atgagattcc aggactgccc cggacgcgcc
    31681 gccgccagtt tgggaatgta gatattggca acgaccgcac caccgtaggc cagtgcgcat
    31741 gcggccaggg cgacgaggac atgccagtca gccagcacac caccggctac ggaaccgagc
    31801 aaaatcgccg ctatggtaga agcttccatt aaaccgttag ctttcactaa cttactaccc
    31861 gtggttaatt cgccgagaat accgtatttc gccggtgaat aggctgcagc accaacaccc
    31921 accagcgtat agccgagaaa cggattgata ccaaagcaga tactggctgc gcccagcagc
    31981 ttcaggccgt tggcaaacat catcacccgg cctttggcga agctatccgc cacctgcccg
    32041 acaaacggcg caaaaagaat gtaagcacct acaaacacca tttgcaggat gggctggctc
    32101 cactccggat agaactgcgc tttcagtaac gccagagtgg caaacagtag ggcattatcg
    32161 ccaaacgcag agagaaactg cgccacgata accgctttca tccccttcga ccacaacgaa
    32221 gtgttagtgt gcactgactc actcatcgtg ttgttccgct tcgtctaccc agcttttcaa
    32281 cgtgacaaag tcaggtttgc cgctgccaag taatggcatc tgtttcagat agcgaatatc
    32341 gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca tactgttgca acttatcgcg
    32401 cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg catcgctctt
    32461 aatcgcagtg gcatggactt tatctggcga aacaccaagt gccagttgtt ccaccatttc
    32521 cagcgacacc atttcgcctg caattttggc aaagcgtttt gcgcggccct gaatctgcac
    32581 aaagccctgc tcgtcaaaac gcacaatatc gccagtgtca taccagccgc gctccatttc
    32641 gccgcgaaca ttctcggcgg tgggcacttc cagtacacct ggcttctcca cccgcagata
    32701 gccgttcatt atgttcggcc ctttcagttg caggcgtccg ccctcttcga taccagggac
    32761 cgacaacagg cgcgcatcca ttcctggtag aatacgccct accgtaccgg gtttcgccgc
    32821 catcggtacg ttgatagaaa cgacaggcgc gcattcggtc acgccgtagc cttcaaggat
    32881 gcgcaggcca aatttatcct gccaaagctg tttggtactt tcttgtaatt tttctgcgcc
    32941 tgccaccaca tagcgtagac gatagaagtc atacgggttg gcgaaacgcg cgtagtgacc
    33001 gaggaaagtc gaggtgccga acaacacggt gcaactgcgg tcatacacca actccggcac
    33061 aatgcggtaa tgcagcgggc ttggataaag gaacacttct gcacctgtaa gcagtggcgt
    33121 aaacaggcct accgtcagcc caaaggagtg aaacagcggt aacgccgaca taaagcgatc
    33181 gttggtggtg aagtcggcaa tcgttttaat ctgctcgaca ttcgccagaa tgcttttatg
    33241 gctatggacg acgcctttcg gatggccttc agaaccggag gtaaagagga tcagcgcctc
    33301 ttcttccggc tgctgtttaa cctgtgccag acgcggcatc agcaaatgag cgaagatcca
    33361 tactttgtcg gcagtggtga catctgcttt taaatcttcc agatagaccc agcgcacctg
    33421 agtaagttgc tccggcagat gccagagttt gcctttatcg agaaactggc gggaagtgaa
    33481 gatggttttg atttcagccg ccgtaatagc actggtcagc ccttttaccc cggcagtgta
    33541 gttcatcatt gcgggcatgc ggcgacgggc gatggcccca aaaatcactg ccgcactgat
    33601 gcctgcattg ggcagcatta agccgatgcg ttcgccttca acactgtatt tttcaaggat
    33661 gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag gagtctgggg taaagttgac
    33721 gtcttcgaca catttcttcc cggctccgaa gcggtacatt gcactcagta aagattcgta
    33781 cagcgtttca cgcgggcgca ccgccattcg cgcttccatc attatttgat gcagcatttc
    33841 gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc ggcatcgcca cctgcgttgg
    33901 tggcaaaata tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac ctttcaggcg
    33961 gctgaagtgc gtaagttccg ccccttcaat acgcacagga ataaccgttg caccagactt
    34021 cgccgcgaca aaacccgcgc catcgtagat tttcatcagc gagcctgtag tggtgatgcg
    34081 tccttcaggg aaaatcacca ctggtcggcc ctgttccacc agacgtacca gatgtttaat
    34141 agccataggt tgcgtcgggt cgagaggaac aaagtcgata aatgatttca gccaacgcat
    34201 ataccactgt tggcttattg aggtgtaaac ggcaaacact ggacgcacag gtaaaaacag
    34261 tccaagcaaa atgccatcaa taaaagagac gtgattaggc gtaattagaa cgcgctcgcc
    34321 cttcagtgcc tgggtgtcac ccgtaacgcg aacgcgatac aaaacacggc acaaatttcg
    34381 aaaaaagcta aaaagcattc caactccctt tgctctgatt cagtaaaagc gaatggaggg
    34441 agattacacg agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa aaacctgcgc
    34501 atccgcgcag gttggtgcaa gagacagggt acgaagagcg taccgaataa tctcaccaat
    34561 caatacctct gggatcttga ttgtggtctg cacgacgact cttcgccagc gagaaaacgc
    34621 aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt acagattgat
    34681 gaccggcaaa aaaaaacctg cgcatctgcg caggctggtg taattcatgt gctcaacccg
    34741 aagttgactt cacctatcaa tacctctggg atcaccactt tagcaacctg aagccaaacg
    34801 ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt
    34861 tttttgtctt acttatttgc gtttggctca cattcccacg atgaaaacac gccacccctt
    34921 gaaccaacgg gcgttttccg taacactgaa agaatgtaag cgtttaccca ctaaggtatt
    34981 ttcatggcga ccataaagga tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc
    35041 cgcgtcatta ataattcacc taaagccagt gaagcttccc ggctggctgt gcatagtgca
    35101 atggagtctc ttagctatca cccgaacgcc aacgcccgtg cgctggcgca gcagaccact
    35161 gaaacggtcg gtctgatcgt tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa
    35221 gcggtcgaac aggtggctta tcacaccggt aatttcttat tgattggcaa cggttaccac
    35281 aacgaacaaa aagagcgtca ggctattgag caactgatcc gccatcgctg tgctgcgttg
    35341 gtcgtccatg ccaaaatgat cccggatgcc gatttagcct cattaatgaa acaaatgccc
    35401 ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa accgttgtat tgctcttgac
    35461 gatcgttacg gtgcctggct ggcaacgcgt catttaattc agcaaggtca tacccgcatt
    35521 ggttatctgt gctctaacca ctctatttct gacgccgaag atcgtctgca agggtattac
    35581 gatgcccttg ctgaaagtgg tattgcggcc aatgaccggc tggtgacatt tggcgaacca
    35641 gacgaaagcg gcggcgaaca ggcaatgacc gagcttttgg gacgaggaag aaatttcact
    35701 gcggtagcct gttataacga ttcaatggcg gcgggtgcga tgggcgttct caatgataat
    35761 ggtattgatg taccgggtga gatttcgtta attggctttg atgatgtgct ggtgtcacgc
    35821 tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg tgacgatggc gacccaggct
    35881 gccgaactgg ctttggcgct ggcggataat cgccctctcc cggaaatcac taatgtcttt
    35941 agtccgacgc tggtacgtcg tcattcagtg tcaactccgt cgctggaggc aagtcatcat
    36001 gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc agtaattctt cgatggtctg
    36061 gcgacggcga atcaaccgcg cctgaccatt atcaaacaga acttctggta acagcggacg
    36121 gctattgtag ttggatgaca ttgatgcgcc atatgcccct gtatcatgca gtaccagata
    36181 atcacccgcc ttcacttccg gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt
    36241 aaagacatcg cccgattcac ataatggccc ggcgacgacg gtttccaccg ttggcgcgtg
    36301 ttccagagaa cgaccatcag ctgccagggc actgatatgg tggtaactac cgtacattgc
    36361 cgggcgcatc agatcgttga acccagcatc aaccagcaca aagtggcggc tacccatttg
    36421 tttgacgctc cgcacctgag taattaatac gccagactgc gctaccagga agcgacccgg
    36481 ttcaatttcc agtttcacag ggtggcccaa atggcgggcg atttgctcac gcgcggcatt
    36541 ccacagacca taataatgtt cggtatcaac cgcctcttca ccctgttgat aaggaacaga
    36601 aagcccaccg cccgcagaga tagcctgtaa atcctgaccg aattcgatga cctgacgcac
    36661 catagcacca cacacctgtt ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg
    36721 aatgccgacc agctgcagat gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt
    36781 gtaccagata ccgtgcttgc tgttttcgcc accggtattg gttttttggc tatgtccgtg
    36841 accaaacccc ggattaacgc gcagccatac ccgatgccct ggcgaaacca ggcccagttg
    36901 gtcgagcata tcaacagaac ccgcattcac cggaatttgc aattcactga cgcgttcaag
    36961 cgtcgcctga tcgataacat ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt
    37021 gtaacccgcc gccaacgcac gctctatttc gcctaacgag acggaatcca ctttcacgcc
    37081 ctgctcacgc attaagcgca aaatatgaat attggaacag gctttctgtg caaagcgcac
    37141 cacatcaaac tgtttcagcg ctgcaatctg ccgacgaata atttgcgcat cgtagaccca
    37201 caccgggcag ccaaattcag cgggcaaacg cagcagattt tcggcggtga gatcggtatc
    37261 ggtgctgaac agtgaatgtg gcataacaaa ctccagataa gtgctttttt atgattacgc
    37321 cacatcataa aaagaataaa aaatatcgat ttatgtcgag tctatgcaaa aatgatatgg
    37381 attaccggat tgcgagagag cgctaatggc cgccgttaac ttacgtcata ttgaaatttt
    37441 tcatgcggta atgaccgccg gaagcctgac tgaggcagca cacctgctac acacctcaca
    37501 gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg atcgggctaa aattgtttga
    37561 gcgcgtacgt ggacgattac atcctaccgt gcaaggactg cgtctgtttg aagaagtgca
    37621 acgatcctgg tacggactgg atcgcattgt cagcgccgca gaaagtctgc gcgagtttcg
    37681 ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg caatcttttt taccgcagct
    37741 cctgcaaccc tttctggcac gttatcccga tgtcagctta aatatcgtgc cccaggaatc
    37801 accgctactt gaagagtggc tctcggccca gcgtcatgat ttaggactca ctgagacact
    37861 ccatacgcct gcgggaacag aacgtaccga attactctct ttagatgaag tgtgtgtgtt
    37921 acctccgggt catccgctgg cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga
    37981 gaactacatc agcctttccc gtactgacag ctatcgccag ttgctggatc agctatttac
    38041 tgaacatcag gttaaacgac gcatgatcgt agaaacccac agcgccgcgt cagtctgcgc
    38101 aatggtacgg gcgggggtag gtgtttcggt ggttaacccg ctcaccgcac tggattatgc
    38161 ggcaagcggt ttagtggtgc ggcggttcag tattgcggtt ccgttcaccg tcagcctgat
    38221 ccgccccctg caccgcccgt catcagcgct ggtgcaggcg tttagtgagc atttacaagc
    38281 ggggttaccg aaactggtca cttctcttga cgctattttg tcgtcagcta cgacagcata
    38341 aaagcgacag catcctcggc atggatcgcc gcagtatcaa acacaggcaa aacactgcgc
    38401 tcttctggca ccagtaaacc aatttctgtg cagccaaaaa tgacgccctg tgcgccctgt
    38461 tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt aaattgcccc
    38521 agacacagtt cttcaaaaat aatctgatta attttcgccc gttcatccgc ttcaggaata
    38581 agacaattga tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg
    38641 taacgcgtac ccagcagcgc cacacgagtc attccggccc cggtaattgc acgtccggtg
    38701 gcatccgcaa tgtgtaagaa aggcagagtg caacgtgact caatggcatc cgccacttta
    38761 tgcatcgtat tggtacataa cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc
    38821 gcctcagcca gaatgtcccc ggttttatcc cattccccgc gacgctggca ctcatctatt
    38881 tcatgaaaat cgacgctatg tagcagcact tgcgcagagt gaagcccacc aagccgctgt
    38941 ttaatgcctt catttatcag acgatagtaa ggaatggtgg attcccagct cattcctccc
    39001 agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt taccgcaaca aggctgaata
    39061 aggaacgaga caaacgcctc aacggccagg tgcccgatct ctattaacga aaaaagggcc
    39121 ggatgtacag cacatccggc ccgtgaaatc agacgccgat atttctcaac ttctcgcctg
    39181 ccatcagttt gcgttcgata tgttccagcg tgacattttt ggtttccgga atgagccaga
    39241 aagtgatgcc cacaaacgca atgttcagcg cagtgtagag ccagaacgta ccggcagcgc
    39301 caatgctatc aagcagtgtc aggaaggtcg cgccgataat catattcgac acccagtttg
    39361 tcgtcgtcga acaggtaata ccgaaatcgc ggcatttcag cggctgaatt tcagagcaca
    39421 ggatccacac cactggcgcg gcgctcatcg cataaccggc aatacacatc atcgtcatgc
    39481 caacagagag ccaggacaag ccactggaag ccgtaccgtt atcaaactgc atcaggcaat
    39541 agcccagcac cagagtgcct aacgccatca cgctgaaacc aattttcaga gccggtttac
    39601 gacctgcttt atctaccgta aagaccgcaa tgaaggtcgc gaacataaag gtcagtccca
    39661 cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt ttgaagatac
    39721 gcggcgcata gtacatgatg atgttcatac cggtaaactg ctgcatcgcc tgcaacaaca
    39781 taccgagaaa cacagcacga cggacgttac ggttgatctt aaacagtgcc caaccgcctt
    39841 gttttaactt caggctttca cgaatttcgt tgagttcttc tcgcgctttt tccgacgtat
    39901 cgcgcagcat acgcaacact tcttccgcct caatatgacg ccccttttcc gccagccagc
    39961 gcgggctatt tggcaggaag accaccagaa taatcagcag gactgctggt aaagcaagaa
    40021 cccccaacat tgcgcgccag ttaccgctat aactgaacgc tgtatcggat aaaaacgcca
    40081 gcacgatgcc gagtgtgacc atcaactggt acatactgat catcttaccg cgaacgtttt
    40141 cacttgccat ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc
    40201 ccagcaccac acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag
    40261 aaccgagtac aaacaggatg gcccccgcca tcaggctgta tttacgaccc aggcggaacg
    40321 acagccaacc attaaacagc gcaccaattg ctgcgccgag catcatgcta ctaaccaccc
    40381 attcctgcaa acgactggtc agcacaaagt gatcggtaat gaacggcaac gctccggcga
    40441 ttacgccgat atcaagacca aataacaatc ctgcgaccgc agcagctacc gaaacaaaca
    40501 tattcatacg ccgcgtatcc cgcaaagaac gtggcgttaa agcagattcc gtattgatag
    40561 taaccatttt ttcctgccag cagagagtaa gacatagtga aaaaatacgt gaacaactca
    40621 cgcaggtgtc aggtcggaaa cagcataaat atggattaaa ttgctgcgac atgtcgttat
    40681 gtgatggata ttccaatttt caaattaagt tgaattattg agattattat taaccaccta
    40741 attttacagc agataaaatt cataaagttc attaattgat aattaatatg gattatttca
    40801 taaccatgat atggattatg atgatctaca ggtataaaaa accctgccat gcggcagggt
    40861 cataaaagta agaagaatga attaacgcgc cagccaaccg ccatccacgg caatggtata
    40921 accattcaca taatctgaag cgctggaggc aaggaacact atcggcccca tcaggtcact
    40981 cggcagtccc caacgaccag ctggaatgcg gtcgagaatt tccgcgctac gttgttcatc
    41041 tgcccgtagt tgttgagtat tgttggtcgc catgtaaccc ggagctatcg cattaacatt
    41101 aatgttgtgt ttagcccatt cgttcgccat caatcgcgtc acacccatca cgccgctttt
    41161 tgatgcggta taagaaggca cacggatccc gccctggaag gagagcattg acgcgatatt
    41221 gataatcttg ccgccattgc cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa
    41281 gaatacgctc ttgatattca ggttcatgac atcgtcccag tctttttcgc tgaactcgag
    41341 agcgtcttcg cggcgaatca atccggcgtt attcaccagg atatcaatat gaccaaattc
    41401 agctaccgcg cgatccagca gtccaggaat gccatcaatc tttcgcagat cggcggtcag
    41461 gcttaaaaaa cgacgcccca gcgccgtgac ctgctcgatg gtttcagtcg gttcaacgat
    41521 gttaatgcca acaatgtcac agcccgcttg cgccagcccc aacgccatcc cctggcccag
    41581 cccagtatca caaccagtga cgaccgcaac tttaccttcg agagaaaatg cacttaaaat
    41641 cataacaata ccttattcgt ttatgcccac aactagcgca aatctttaac ggccacatgg
    41701 tccatatcat caaagacctg gttttcaccg accatgcccc agataaaggt ataagctttg
    41761 gttccgacgc cggaatgaat cgaccagctc ggggagatca ccgcctgctc gttatgcatc
    41821 acaatgtgac gcgtttcttg cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc
    41881 atattgaaat agaaataaac ttccatccgg cgctcgtgag tgtgacacgg catggtgttc
    41941 cacaagttac ctggagccag ctccgtcagc cccatactca attggcaggt ttccagcaca
    42001 tccgggacaa aatatttgtt aattgtgcga cggttactgg tgaggttatc gcctaacgtg
    42061 actggagata cttcgtccgg tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg
    42121 caattgtaat aaaactttgc cggagtggcg gtatcaatac tggcaaagac aacttctttt
    42181 gcacctttac ccacatacag ggcgtcgcgg tgaccgattt catagcattg gccatcgaca
    42241 gtaatcgtac cggcaccgcc aatattgata acacctaact cgcgacgttc aaggaaatag
    42301 cttacgccga gttgtttacc aacttccccg ccaacggaaa ccgttttagt tatcggcata
    42361 atgccgccaa caataattcg gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca
    42421 aatacctctt caaccaaaaa ttcattgcgc agcccttggg tatccagcgt ttttgcgtgc
    42481 gcactgtgga tgctctgtct tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa
    42541 atagaaaacg atagtgaaac actgtttcgt tttctctata aagggatgat atgcccatgg
    42601 gctagggttt tcaattaaaa ataaaacaat gtttcatttt taagttaagg attaaaaaaa
    42661 gtgcagtgtc gatcacgaat atgtcatgag aatggaaaaa tgagggagcc cagaaaatgt
    42721 atgtcgactt gccagccatc attattcaac agattaatga cggcagatat ttatcggtaa
    42781 ggaggaaatg cagagctact cgtcacgttc aatggtcaat gccacgccct ggcccccgcc
    42841 gatacaaagc gttgccagcc ctttgcgggc attacgtttc accatttcat gaaccagaga
    42901 aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt
    42961 gacattgacc cgacgctcat cccactcaag catcttgcca accgaaagcg cctgtgcagc
    43021 aaacgcttca ttagcctcga taagatcgac atcagccaac tgccagccta cacgctccag
    43081 gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct
    43141 ggcaaatgcg cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct
    43201 catcatcatt accgcagctg cgccatcgtt tatggatgat gcattccccg cggtcacaga
    43261 accgagacta tcaaatgaag gatttaaacg ggctaaacct tctgcactgg cgtcagtgcg
    43321 tggctgttca tcggtatcaa caaccaacgt ctgaccgtta ctttggatca ttaccgggac
    43381 gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag
    43441 tgcgtaagca tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt
    43501 gacaccaata tgataatcat tgaaggcatc ccacaaccca tcatgcacca gactgtcaac
    43561 caactggcta ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg
    43621 gctcatgttt tcctggccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg
    43681 agtagccaga tgcagtgctt taagcccaga accgcaaacg tcattaatag tgattgcaga
    43741 aacgctatta ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc
    43801 tgcagtcaac acctgaccaa gaattacttc atccaccgca tatgcaggaa cgccggtacg
    43861 ttctattaac gctttcacga ccagactacc aagttccacg gcggaatgac ccgctaacgc
    43921 accgcgaaag cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac
    43981 aacctcgcac caattaacag tgcaatagta aatgattgtt aattacagtt atcttaattg
    44041 tttaaaaaaa gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa
    44101 ctggcacgta aggtttgcaa ccactaaccc accaatagag gggtagatag ggcgttaatc
    44161 tcccatactt aacctggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat
    44221 acgttcagat aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct
    44281 acggcacagc cgttggtgca ggcacgctct tccttcctat tcagttaggt tcggcagggg
    44341 ctgtggtcct gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag
    44401 ccttatgcca gttcatcctc tcatcgaaaa catcagcagg tgaagggata acgggcgcag
    44461 taacacacta ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct
    44521 tttttgtcgt cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg
    44581 caaagcatat ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa
    44641 tcctgaatct catttttctg atgggacgcc atgcaaccat tcgggtaatg ggttttttgg
    44701 tattcccatt gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac
    44761 ctgatctatt aacgacccag gtagagttca atcagaatac ccttcaccag atatggatat
    44821 cgattcccgt gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag
    44881 acagacgtga aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg
    44941 cttatctcat catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc
    45001 caccttcgta tattgaagcg gctaaagaag aaggggtcac cattttatca gcgctttcta
    45061 tgctgccgaa cgccccagca tggctgtcaa tttccgggat tattgtcgca gtagttgcga
    45121 tgtcgaaatc attcctgggt acgtactttg gcgttattga aggtgccacg gaggtcgtca
    45181 aaacaacact acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccgc gcactatcaa
    45241 ttatgttggt atcgctgatc accttcattg tttgttgcat taacccgaac gcgatttcga
    45301 tgatttacgc gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgttgt
    45361 caacgtatct gatcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga
    45421 tcgtgggtat cctgtgcgta tcggtaatgt tctttagctg atatccattg tgaatggttc
    45481 aaaattgcta caattttgaa ccattccagt tttcataatg ttcattcgtt attattccgc
    45541 actatcactt ccctcatcat atcctagtat tatcagttag ttaaacacac aaagagatct
    45601 gaacatctta aataagacta tttaagatgc ataacttaga ttcgcaagat atcttccggt
    45661 cattcaaaaa caatttgcga tatagatatt ctcataccat gagatagtta tctgaaccac
    45721 cctcaaccag gtaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat
    45781 tttaaaatgt cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa
    45841 aattacaaaa tcatggtgta ttgacatcat caatcaccaa tgaaattatg caattatata
    45901 cggataggga ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata
    45961 gttgctcaat gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa
    46021 actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag
    46081 gtatcttaac aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag
    46141 taagtttttt acgtctattc cttgaaaaag actggattga ctatcttgca agtactgaca
    46201 tggggattgt tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac
    46261 acaactcagc aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa
    46321 agatcagaca actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc
    46381 agatggaatt caccataatg gggtatatgg tttctggtta taatccatat cagattgctg
    46441 aagttctgga tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa
    46501 tgggtggtaa aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa
    46561 cgaccagcca gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata
    46621 aataggccgt cccctcaaga gtaaacacca tagtatttaa aaccatcaca cgaaaaattc
    46681 agaagcatta cgaaatatga atttacaaac aatagtggca taaatgttaa ccatgttaat
    46741 ttacgtaaag ttttacgttg caacattaaa gcctcatttc aatcatcatg ataaatataa
    46801 aattaatata tatttatgcc gtaaatacca atatacttag caaactatgt gatctccatt
    46861 tcgattgatt tagtgtttat tgacgtatgt actggattat taacgataat atcgagttct
    46921 ggcctgagca ccgaaaatta atatcggtac ataacgccga tcttaacgtc gttctgacaa
    46981 cgccagccag tcgatgttta tcacttctac ttgaagcttt tcctgatgtg gttgcacaac
    47041 aagatttttt caccagagtc tgggaagaag aaggtatgcg tgtgcctact aacacgttat
    47101 atcagaacat atccattatc agacgcggat ttcgcgctgt tggtgatact acccactcgc
    47161 taattgcaac cgtgccgaga agaggattca agatccataa tgacactaac atacaaaatc
    47221 atgtaataaa ctcgtcaaca gacgcacata cacacaatgc cccacctgcc ataaaagtta
    47281 atgcgggata caaagagagc attggtggcg caaagaattt taataacaaa atcctcaaac
    47341 atataaaaag tcatctaatt atgttgagcg catttgtcat aggcgcatat tctgcatatt
    47401 ggctatggaa taataatcaa ccgaagccat ttttcaagga ttacaagact gttgcggaaa
    47461 ttaatggctg ccattttaat gtaacagaag atacaattga cgggttgaaa gagttcgata
    47521 aatataaaac acggatactg gattcgggga ttaactgtaa aaaacatccg tggttatact
    47581 tccctcttgc aaaatcctca ccggggatga ttgttatggc atgcaataaa aattataacc
    47641 aacatgaagt ggcagactgt ttaaccct
//
LOCUS       DAZGCS010000025        46794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGCS010000025 DAZGCS010000000
VERSION     DAZGCS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.025"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(99..830)
                     /gene="rlmB"
                     /locus_tag="ACHK01_001505"
     CDS             complement(99..830)
                     /gene="rlmB"
                     /locus_tag="ACHK01_001505"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO8096478.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1010..3451)
                     /gene="rnr"
                     /locus_tag="ACHK01_001506"
     CDS             complement(1010..3451)
                     /gene="rnr"
                     /locus_tag="ACHK01_001506"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO8096479.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3490..3915)
                     /gene="nsrR"
                     /locus_tag="ACHK01_001507"
     CDS             complement(3490..3915)
                     /gene="nsrR"
                     /locus_tag="ACHK01_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO8096480.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4120..5418)
                     /gene="purA"
                     /locus_tag="ACHK01_001508"
     CDS             complement(4120..5418)
                     /gene="purA"
                     /locus_tag="ACHK01_001508"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO8096481.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5522..5719)
                     /gene="yjeT"
                     /locus_tag="ACHK01_001509"
     CDS             complement(5522..5719)
                     /gene="yjeT"
                     /locus_tag="ACHK01_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO8096482.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5801..6805)
                     /gene="hflC"
                     /locus_tag="ACHK01_001510"
     CDS             complement(5801..6805)
                     /gene="hflC"
                     /locus_tag="ACHK01_001510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO8096483.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6808..8067)
                     /gene="hflK"
                     /locus_tag="ACHK01_001511"
     CDS             complement(6808..8067)
                     /gene="hflK"
                     /locus_tag="ACHK01_001511"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO8096484.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8153..9433)
                     /gene="hflX"
                     /locus_tag="ACHK01_001512"
     CDS             complement(8153..9433)
                     /gene="hflX"
                     /locus_tag="ACHK01_001512"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO8096485.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9510..9818)
                     /gene="hfq"
                     /locus_tag="ACHK01_001513"
     CDS             complement(9510..9818)
                     /gene="hfq"
                     /locus_tag="ACHK01_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO8096486.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9904..10854)
                     /gene="miaA"
                     /locus_tag="ACHK01_001514"
     CDS             complement(9904..10854)
                     /gene="miaA"
                     /locus_tag="ACHK01_001514"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO8096487.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10847..12694)
                     /gene="mutL"
                     /locus_tag="ACHK01_001515"
     CDS             complement(10847..12694)
                     /gene="mutL"
                     /locus_tag="ACHK01_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO8096488.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12704..14041)
                     /gene="amiB"
                     /locus_tag="ACHK01_001516"
     CDS             complement(12704..14041)
                     /gene="amiB"
                     /locus_tag="ACHK01_001516"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO8096489.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14060..14521)
                     /gene="tsaE"
                     /locus_tag="ACHK01_001517"
     CDS             complement(14060..14521)
                     /gene="tsaE"
                     /locus_tag="ACHK01_001517"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO8096490.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14493..16040)
                     /gene="nnr"
                     /locus_tag="ACHK01_001518"
     CDS             complement(14493..16040)
                     /gene="nnr"
                     /locus_tag="ACHK01_001518"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO8096491.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16039..17178
                     /gene="queG"
                     /locus_tag="ACHK01_001519"
     CDS             16039..17178
                     /gene="queG"
                     /locus_tag="ACHK01_001519"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO8096492.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17161..17214)
                     /locus_tag="ACHK01_001520"
     CDS             complement(17161..17214)
                     /locus_tag="ACHK01_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096493.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17448..17523)
                     /locus_tag="ACHK01_001521"
     tRNA            complement(17448..17523)
                     /locus_tag="ACHK01_001521"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17488..17490),aa:Gly,seq:gcc)
     gene            complement(17559..17634)
                     /locus_tag="ACHK01_001522"
     tRNA            complement(17559..17634)
                     /locus_tag="ACHK01_001522"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17599..17601),aa:Gly,seq:gcc)
     gene            complement(17845..18390)
                     /gene="orn"
                     /locus_tag="ACHK01_001523"
     CDS             complement(17845..18390)
                     /gene="orn"
                     /locus_tag="ACHK01_001523"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8096494.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18485..19537
                     /gene="rsgA"
                     /locus_tag="ACHK01_001524"
     CDS             18485..19537
                     /gene="rsgA"
                     /locus_tag="ACHK01_001524"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8096495.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19634..20602
                     /gene="asd"
                     /locus_tag="ACHK01_001525"
     CDS             19634..20602
                     /gene="asd"
                     /locus_tag="ACHK01_001525"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8096496.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20624..23947
                     /gene="mscM"
                     /locus_tag="ACHK01_001526"
     CDS             20624..23947
                     /gene="mscM"
                     /locus_tag="ACHK01_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8096497.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24098..25600)
                     /gene="yjeM"
                     /locus_tag="ACHK01_001527"
     CDS             complement(24098..25600)
                     /gene="yjeM"
                     /locus_tag="ACHK01_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8096498.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25819..26796)
                     /gene="epmA"
                     /locus_tag="ACHK01_001528"
     CDS             complement(25819..26796)
                     /gene="epmA"
                     /locus_tag="ACHK01_001528"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8096499.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27121..28929
                     /gene="frdA"
                     /locus_tag="ACHK01_001529"
     CDS             27121..28929
                     /gene="frdA"
                     /locus_tag="ACHK01_001529"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8096500.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            28922..29656
                     /gene="frdB"
                     /locus_tag="ACHK01_001530"
     CDS             28922..29656
                     /gene="frdB"
                     /locus_tag="ACHK01_001530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8096501.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29667..30062
                     /gene="frdC"
                     /locus_tag="ACHK01_001531"
     CDS             29667..30062
                     /gene="frdC"
                     /locus_tag="ACHK01_001531"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8096502.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30073..30432
                     /gene="frdD"
                     /locus_tag="ACHK01_001532"
     CDS             30073..30432
                     /gene="frdD"
                     /locus_tag="ACHK01_001532"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8096503.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            30495..31628
                     /gene="ampC"
                     /locus_tag="ACHK01_001533"
     CDS             30495..31628
                     /gene="ampC"
                     /locus_tag="ACHK01_001533"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8096504.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31717..32250
                     /gene="blc"
                     /locus_tag="ACHK01_001534"
     CDS             31717..32250
                     /gene="blc"
                     /locus_tag="ACHK01_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8096505.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32247..32564)
                     /gene="sugE"
                     /locus_tag="ACHK01_001535"
     CDS             complement(32247..32564)
                     /gene="sugE"
                     /locus_tag="ACHK01_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8096506.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32746..32892)
                     /gene="ecnB"
                     /locus_tag="ACHK01_001536"
     CDS             complement(32746..32892)
                     /gene="ecnB"
                     /locus_tag="ACHK01_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8096507.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33003..33128)
                     /gene="ecnA"
                     /locus_tag="ACHK01_001537"
     CDS             complement(33003..33128)
                     /gene="ecnA"
                     /locus_tag="ACHK01_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8096508.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33180..33746)
                     /gene="efp"
                     /locus_tag="ACHK01_001538"
     CDS             complement(33180..33746)
                     /gene="efp"
                     /locus_tag="ACHK01_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8096509.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33788..34816
                     /gene="epmB"
                     /locus_tag="ACHK01_001539"
     CDS             33788..34816
                     /gene="epmB"
                     /locus_tag="ACHK01_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8096510.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35206..36075
                     /gene="yjeJ"
                     /locus_tag="ACHK01_001540"
     CDS             35206..36075
                     /gene="yjeJ"
                     /locus_tag="ACHK01_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8096511.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(36278..36631)
                     /gene="yjeI"
                     /locus_tag="ACHK01_001541"
     CDS             complement(36278..36631)
                     /gene="yjeI"
                     /locus_tag="ACHK01_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO8096512.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(36769..38415)
                     /gene="groL"
                     /locus_tag="ACHK01_001542"
     CDS             complement(36769..38415)
                     /gene="groL"
                     /locus_tag="ACHK01_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO8096513.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(38459..38752)
                     /gene="groS"
                     /locus_tag="ACHK01_001543"
     CDS             complement(38459..38752)
                     /gene="groS"
                     /locus_tag="ACHK01_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO8096514.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            39028..40284
                     /gene="yjeH"
                     /locus_tag="ACHK01_001544"
     CDS             39028..40284
                     /gene="yjeH"
                     /locus_tag="ACHK01_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO8096515.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(40300..40776)
                     /gene="fxsA"
                     /locus_tag="ACHK01_001545"
     CDS             complement(40300..40776)
                     /gene="fxsA"
                     /locus_tag="ACHK01_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO8096516.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            41113..42549
                     /gene="aspA"
                     /locus_tag="ACHK01_001546"
     CDS             41113..42549
                     /gene="aspA"
                     /locus_tag="ACHK01_001546"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO8096517.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            42667..43968
                     /gene="dcuA"
                     /locus_tag="ACHK01_001547"
     CDS             42667..43968
                     /gene="dcuA"
                     /locus_tag="ACHK01_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO8096518.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            44084..44422
                     /gene="cutA"
                     /locus_tag="ACHK01_001548"
     CDS             44084..44422
                     /gene="cutA"
                     /locus_tag="ACHK01_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO8096519.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            44398..46095
                     /gene="dsbD"
                     /locus_tag="ACHK01_001549"
     CDS             44398..46095
                     /gene="dsbD"
                     /locus_tag="ACHK01_001549"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO8096520.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            46132..46707
                     /gene="yjdC"
                     /locus_tag="ACHK01_001550"
     CDS             46132..46707
                     /gene="yjdC"
                     /locus_tag="ACHK01_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8096521.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT        11144 a        12641 c        11832 g        11177 t
ORIGIN      
        1 tattaataaa atgaatattt gcagtacgta attatcttac cagctataga caaaaaaaaa
       61 ccatccaaat ctggatggct tttcataatt cagacgaact agctgcgctg acgaaccgct
      121 tcaaataagc aaattccggt tgcaaccgaa acgttcaggg aagaaacact tcctgccatt
      181 gggatgctga tcaactcatc gcaatgttca cgggtcaggc gacgcatacc ttcaccttcc
      241 gcgcccatca ccagcgccag gcgtccggtc attttgctct gatagagtgt atgatccgcc
      301 tcgcctgccg taccgacgat ccagatattc tcttcctgca acatacgcat ggtgcgcgca
      361 aggttagtca cccgaatcag tggaacgctt tctgccgcgc cgcaggctac ttttttcgcc
      421 gtagcgttga gctgtgcgga gcgatctttc ggcacaatca ccgcgtgaac gccagcagcg
      481 tccgcgctac gcaggcacgc gccgaggttg tgcggatcgg ttacaccgtc gaggatcagc
      541 aggaacggtt gatcgagcga agcgatcaga tccggcagat cgttttcctg atactgacgt
      601 cctggcttca cgcgggcgat aatgccctga tgcacggcac cgtcgctttt ctcgtcgaga
      661 tattggcggt ttgccaactg gataaccacg ccctgagact caagggcgtg aatcagcggt
      721 aacagacgtt tatcttcacg gccttttaaa ataaagactt cctgaaaacg ttcaggggcg
      781 cgctccagca gggcctgcac tgcgtggatg ccgtaaatca tttcgctcat taatgtactc
      841 gttgtttacg ttttgcctga tgcactacgt ttatccggcc tacatgatct ctgcaatata
      901 ttgaatttgc gtgattatgt aggccggata aggcgttcac gccgcatccg gcatgaacaa
      961 agcgcatttt gtcagcaatc taaccctctt cttttaaaga gggtattgat cactctgcca
     1021 cttttttctt cgccgcacgc ttcgctttgg tcgctgcggc tattttctgc gttttcgccg
     1081 atggcttttt cgcttttctc gcgtctttct tcgccgcttt cggcttcgtt tttttctcac
     1141 cgcggaaggc gctgtctggc tcaaagttta cctttttacc gacctgacga cgcttgccgc
     1201 cttttttgcc tgcatcgcct tttttcgctt tctcgcgcgc cgttttaccg acgttgcgcg
     1261 gtgcgcgttc actggagatc agactaaagt cgattttgcg ctcgtccata ttaaccgctt
     1321 cgacgcgaac ttccacgcga tcgcccaggc gataagtctg gccgctggat tcccccatca
     1381 ggcgttgccc tacctggtca aagcgatagt agtcattgtc cagcgaagag acatggacca
     1441 gaccatcaat gaacaagtcg tccagacgga cgaagaagcc aaagccagtg acgctggaaa
     1501 ttacgccttt aaagacgtta cctacctggt cgagcatgaa gtcacacttc agccagtcag
     1561 cgacatcgcg cgttgcttcg tcggcacgac gttccgccat cgaacagtgc tgacccagtt
     1621 gcaacatctc ttccatcgaa taatggtagc cgccggtttc agtggtgttg ccctgatgcc
     1681 cctgctcttt cgacagcaga tatttaatgg cgcggtgcag cgtcaggtca ggataacgac
     1741 gaatcggcga agtaaagtgc gcataggact gcaatgccag accgaagtga ccacggtttt
     1801 ctggatcgta aatcgcctgt ttcatcgagc gtagcagcat ggtttgcagc atttctgcat
     1861 caggacggtc ggcaaccgac tccagcagtt ccgcgtagtc acgcggttcc ggcttgttac
     1921 cacccggcag ctccagcccc agctccgcca gcactgaacg gaaagaggta atcgcttcgg
     1981 tgctcggctt gtcgtgaata cggaacagtg ccggttcttt cgctttctca acgaaacgcg
     2041 ccgccgagat attcgccaga atcatgcact cttcaattaa tttgtgcgcg tcgttacgct
     2101 gggtctgttc gatacgttca atacggcgtt cagcgttgaa aatgaacttc gcttcttcgc
     2161 tctcaaatga gatcccaccg cgttcttcac gggctttatc cagcacttta tagaggttat
     2221 gcaactcttc gagatgctta accagcgggg cgtactgctc gcgcagatcc tgatcgccct
     2281 gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg agagctcatc accgcttcgt
     2341 agaatttgta gcccgtcagg cggcctttcg acgaaaccgt catctcgcac accatacaca
     2401 ggcggtctac ctgcgggttg agcgaacaca ggccgttaga gagcacttcc ggcagcatcg
     2461 ggataacctg cgaagggaag tacaccgacg tgccacggtt acgcgcttct ctgtccagcg
     2521 gcgttggcgg acgcacatag tagctgacgt cggcaatcgc gacccataaa cgccagccgc
     2581 cgccgcgttt tttctcgcag taaactgcat cgtcaaagtc acgggcgtct tcgccatcaa
     2641 tggtgaccag cggtaaatcg cgcaaatcga cacggcccgc ttttgcttct tccggcactt
     2701 cttctttcag cccggcaacc tgttgctcaa cagcctgcgg ccagatgtac ggaatttcat
     2761 gggtacgcag agcgatatca accgccatgc cggtgcccat attatcgcca aggacttcga
     2821 cgattttacc caccgctttg gtgcggcgag tcggacgctg agtcagttcg actacgacca
     2881 caaagcccat ccgcgcgccc atgatctgat cgggcgggat taagatatcg aagctcagac
     2941 ggctatcgtc aggaaccaca aagccgacgc ccgcttcggt aaagtagcga ccaacaatct
     3001 ggctggtttt tggcaccagt acgcggacaa tacgcgcttc acgacgacct ttacggtcag
     3061 cgcccagcgg ctgagccagc acctgatcgc catgaatgca ggttttcatc tgctcgctgg
     3121 agagatacaa atcatcttta cgcccttcaa cccgcagaaa gccgtagcca tcacggtggc
     3181 caataacggt acctttcacc aggtcgaggc gttccggcag cgcatagcac tgacgacgag
     3241 tgaagaccag ttgaccatcg cgctccatcg cgcgcaggcg gcgacgcagg ccttcaagct
     3301 gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc acggctggcc ggtttttcac
     3361 gtttggttaa atgttcgagg ataaattccc gactagggat gggattcgcg tatttttcag
     3421 cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc tccgttgtca tctctgatga
     3481 agattttcgt cactccacca gcaataattt ataaagcggt tgattctctt caaccaaatc
     3541 ggcaagcgtg tagttatcca gttccgtaag aaaactttgc acggccttag aaagcgcctg
     3601 tttcaaccga caggcaggtg taatgtggca aaactcactg ctgcaattca ccagcgataa
     3661 gggctccagc tcgcgcacca catcaccaat acgtatcgca ctcgccggtt tacccaggcg
     3721 aatgccgcca ttttttccac gaacagcagt cacgtagccg gcacgactaa gttgattgat
     3781 tattttgacc atatgattac gggagacgcc gtagacgtca gtcacttcag aaatactggt
     3841 catccgccct tctggcaatg acgccatgta gatcagcgca cgtaatccgt aatcagtgaa
     3901 actcgttaac tgcacatcaa cctcaaaagg gaaatcggga aaaaatacat ttatattgat
     3961 gataaaccag ccacaagctg tgtcgctaat ttatttcagt tcggggaagg aaaaaagcga
     4021 ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga cacagcaatg tatcacactg
     4081 ttagcccggc aggcaaaata tctgccaggc gtaccagaat tacgcgtcga acggatcgcg
     4141 cagaatcatg gtttcagtac gatccggacc ggtagagatg atatcgatcg gcacaccagt
     4201 cagctcttca atacgcttga tgtagttcag cgccgcctgc ggcagaccgc tacgatcttt
     4261 cacgccgaag gtggattcag accagcccgg catggtttcg taaatcggct ctacaccttt
     4321 ccagtcgtca gctgccagcg gagtggtcgt cacttcacga ccatccggca tacggtaagc
     4381 cacacagagt ttcacctctt tcaggccatc cagaacgtcc agtttagtca ggcagaagcc
     4441 agacagggag ttcagctgta ccgcacgacg aacggcaacg gtgtccagcc agccggtacg
     4501 acgacgacgc cccgtagttg cgccgaattc gttaccctgc ttgcagagga actcgccagt
     4561 ttcatcaaac agttcggtcg ggaacggacc tgcacctaca cgagttgagt aagctttgag
     4621 gatacccaga acataatcaa cataacgcgg gcccaggccg gaaccggtcg ccacgccacc
     4681 agcagtggtg ttggaagaag ttacgtacgg ataagtaccg tggtcgatat ccagcagcgt
     4741 accctgcgca ccttcaaaca tgacgaaatc gccacgctga cgcgcctggt cgagcaggtc
     4801 agaaacgtca accaccatag aagtcaggat gtcggcaaca gccatcgtat catccagaac
     4861 tttctggtaa tcaaccgctt cagctttgta gtagttaacc aactggaagt tgtgatattc
     4921 catcacttct ttcagttttt cagcgaaggt ttctttgtcg aaaaggtcgc caacacgcag
     4981 accgcgacgt gctactttat cttcataagc aggcccgata ccacgaccgg tggtgccgat
     5041 cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc gcaacgtgat aatcaaggat
     5101 cagcggacat gcttcagaca gcagcagacg ctcacgaacg gggatgccac ggtcttccag
     5161 ttctttcatc tctttcatca gcgcggccgg agacagcaca acaccgttac cgatgatgct
     5221 ggttacattc tcgcggagaa tacctgatgg aataagatgg agaacggttt tttcaccgtt
     5281 gattacgaga gtatggcctg cgttgtgacc gccctggtag cgtacaacat atttagcccg
     5341 ttcagtcaga agatcgacga tcttaccttt accttcgtca ccccattggg tgcccagtac
     5401 gacgacgttg ttacccattt ttcaaaatca ccgtttgctt aaaaatggat tctaccatcg
     5461 ctttttcaga gttacagcac tttttgcact caaaaatgac ctcgatcagt ctgttttttg
     5521 ttcagccaat cgttttcctc aacatgtagt agaccacaac gcccgcaacc acaagtccac
     5581 cgccaaaacg acgtaaaata ttatcgggca aattggtcat cgcagagatc atcttcttcc
     5641 atgccttcgg gtaaagcatc ggccctaaac cttccagtac caaaaccagg gcaagcgcca
     5701 gccagattgt cgaattcatt cattatcctt atagaaaaag aaaaccaccg acatccctga
     5761 ggatgcggtg gctttattga cctgtaccgc agtcgttata ttaacgcgtt gcggaagtcg
     5821 gcgtcttcat gtagcggaag aaatcgctat ccgggctcat gaccatcacg tcctgattgc
     5881 cagagaagct gttctcataa gcacgcaggc tacggatgaa tgcgtagaag tccggatctt
     5941 tactgaatgc atcagcaaac agtttggctg cttcggcatc gccttcacca cgcatgatgc
     6001 ggccctgacg ctcagcttct gccagcgttc tggtcacttc atagtcggca gtcgcgcgca
     6061 gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg cgctaccgct tcacgctcgg
     6121 cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg caggttgatc tgcttaattc
     6181 gcacatcgac aacctcaata cccagcgccg ccatactgtt cgggttgatg accggaactt
     6241 tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc aatggcgtta tctgccgccg
     6301 gggtagtaac ttcatcttct gtacccgcag aaccggagtt cagcgcgtca cgtacttcga
     6361 gggtcagacg accacgggaa tcggtgacga tatctttcac gtccaggcga ccaatttcag
     6421 aacgcagacg gtcagagaac ttacgtttca acagcacttc cgcttgcgaa atgtcgccac
     6481 cacccgttgc caggtagtaa cggctgaaat cgctgatgcg ccatttgatg taagagtcga
     6541 cgatcaggtc tttcttctct ttggtcacaa agcggtcggc ctggttgtcc atggtctgaa
     6601 tacgtgcgtc gagcattttc accgtttcaa tgaacggtat cttgaaatgc agacccggct
     6661 cataaaccag aggtttgttg tcatcgtcac gcagtacctt accaaaacgc agcgtaatac
     6721 cgcgctcacc ttctttgacg acaaagacag acatgtaaag cactaccagc acgatgatga
     6781 taatcgcgat aactgactta cgcatcgtta ttccccctga cgctggtagt cgttacgctg
     6841 cgcgttggcg cggcgttggt ccataatatc gccctgactg gtggacgacg tgttgcttgc
     6901 tccactggtt gtggaggaag aggctggcgg cagacgcagc agattgctgg taccgttatc
     6961 gctcttcgcc gcaggggcgt taccgccttt cagcatctgg tctaacggca gaaccatcag
     7021 gttgccacct ttatcgttaa ccagcacttt gcgggtgtta cccaacactt tttccatcgt
     7081 ctcgatatac agacgctcgc gagtaatttc cggcgcggct ttatattccg gcagaagttt
     7141 agcaaagcgc gccacttcac cctgagcttc caggatggtc tgggccttgt acgcacgcgc
     7201 ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc tgaacttcgt tggtatacgc
     7261 ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg gcaatcgcat cgtcaaacgc
     7321 cgcttttact tcttccggcg gacgagcagc ctggaagttg acgtccagca gcgtgatacc
     7381 catgtcatac ggacggatag tctcttccag ttcgcgctga gtatcgctac gaatcacggt
     7441 acgaccttcc gtcagaatgc ggtccatggt gtatttaccg ataaccccgc gcagggcgct
     7501 gtcggtagcc tgacgcaggc tgtcatccgg gctggtcacg ctatacagat atttttccgg
     7561 attggtgacg cggtactgca cgttcatctc aacgcgcact acgttctcgt ccgacgtcag
     7621 catcacacca gaagcggcca gttcacgcac ggcttccacg ttcaccggtt tgacttcgtc
     7681 gataaacgtc ggtttccagt tcagacccgg ctcaaccaga tggctgaatt taccaaagcg
     7741 tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa ccactggccg cccagataat
     7801 gacaatcgct gccgctgcga tggtaacgac acgaccgcca agctgcgggc gcgggccttg
     7861 cgatgaactg ccaccgccag atccggtgcc tttaccgccg cccagaccac cgagcttttt
     7921 gctcagtttg cggaagatat catctaaatc aggtggccct tgatcgcgac cgcctttgtt
     7981 tccatttccc tcagagttgc cgccaggttt gctgcttccc cacgggtcgc ggtcttgtcc
     8041 gttattaccg ggctgattcc acgccatgtt tgtgctccat atttgttatg cggtgatccc
     8101 tgttgtcttt cgacgccaga ggatatgact ccacgcttca gacgctacgc cgttagatca
     8161 ggtaatcgat caacgccggt tcttgtttac agaggcgacg ccagtcaacg atcggcatac
     8221 gaacttgcag acttacgctg ccgtcctcct ccatccactc tttttctatt gcctgaagct
     8281 gataaaaacg acttctcaga cgcccttcct gcggcggcaa acgcaatgta tgctgcgcca
     8341 cctcgccgga aagccgctcc gtcaaagcct gaaaaagctg tggtatcccc gctccggtct
     8401 gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc ccgatcaata cgcggttcga
     8461 aatcttccag catatcgatc ttgttcatca ccagcagggt tgggatctcg tgagcgtcga
     8521 tctcttcaag aaccgtattc accgcttcga tgttttcttg tacacgcaca tccgccgcat
     8581 caatgacgtg cagcagtaat gtggcttgcc gcgtctcttg taacgtggct ttaaatgccg
     8641 ccaccagatc gtgcggcagg tggcgaataa accctacggt atctgcaagt acggtttcac
     8701 cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag ggtggcaaac aactggtctg
     8761 ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt agatttaccg gcgttggtat
     8821 atcccaccag cgaaacagta ggaacgtcgg ctttgatacg cgattgccgc ccctgctcac
     8881 gctgcttttc aactctttcc aggcgtgact gtatctgcac gatgcgatta cgcaacaaac
     8941 gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa acctatcccg cctttctgtc
     9001 tttcaaggtg ggtccagcca cgcaccaggc gcgtagccag atggcgcagc tgcgccagct
     9061 caacctgcaa cttaccctca tgggtacgcg cacgttgggc gaaaatatct aaaataaggc
     9121 cggtgcggtc gataacacga cactcgcaca aacgctccag gttacgctct tgcgccgggc
     9181 tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc tttgacagct tccgcaattt
     9241 caactgcttt accttcacct acaaaatact ttgggtgcgg cgctttacgg ctaccggtaa
     9301 tcacctgcaa tgcttcgaca ccggcggaag agaccagaga ttcaaactcc tggaggtctt
     9361 ccatatcttt gtcttgcgta aaatagatgt gtaccagtac cgcctgctca ccagcatcat
     9421 aacggtcaaa caagcgtata accctctaaa tagatcagcg gggaacgcag gatcgctggc
     9481 tccccgtgta aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     9541 gaagtattct gcgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     9601 ttactgtgat gagaaaccgg gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc
     9661 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     9721 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     9781 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     9841 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9901 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9961 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
    10021 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
    10081 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    10141 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    10201 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    10261 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    10321 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    10381 tgctgccgca accggatcta cctcctgaag ttgacgatgc aatgactccc aaccttgctc
    10441 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
    10501 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    10561 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    10621 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    10681 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    10741 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    10801 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    10861 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10921 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10981 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    11041 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    11101 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    11161 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcggcagaaa
    11221 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    11281 attgtgcctg acgtagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    11341 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    11401 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    11461 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    11521 tcggccaggg ggcagccgga cgactgcctg atgctggcgc aggagtgtag cgcggagcta
    11581 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    11641 gaatggaacg cggtgcaggt tggggttcat cgtccagcgg tagcggcgtt tccagttgct
    11701 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    11761 aacgcacttc gtgtttggcg gggtgcacgt tgacgtccac ctgatgcgga tcgatctcca
    11821 gatacagcac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    11881 tcgcgtgatt gatcaggcga tcgcgcatca tgcgaccgtt cacgtagcaa tactgaattt
    11941 ctgccagtgc gggcgtggtg tgatttggat cggccaccca gccgcgtagc gtgagatcgc
    12001 cgtgttgcca ttcaatcgcc agcgcttgtt caagaaaagc ggtgccgcaa atcgcgccta
    12061 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    12121 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatctcat
    12181 caatgtggtt aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    12241 agaacagatc cagcacctcc agcgtcgtcc ccacaggatg cgccgccggt tttaccgtca
    12301 cgttcatatc gcgcccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    12361 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    12421 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgcgcca
    12481 gcgccagcgc cagctcgtct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    12541 tcgccccacc gcgttcgata tcaatatcga tacgtgtcgc acctgcatcg aggctgtttt
    12601 ccaccagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    12661 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    12721 gatctggcgt cgtcaccgta ctggccgttt gtgccgttgc cccctgcggc gcagattgca
    12781 tcggatgcgc aaggaaataa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    12841 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12901 ccagtactga tgggatatcc ggcgaacgca gaacgccgag actcgcgtgt tctggtcggc
    12961 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    13021 ctacccgttg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    13081 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    13141 gctccagcca gctggccatt tcgctgttgg cgcgacggtt agagagcacc catacggaag
    13201 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    13261 tttgcttacg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    13321 ttaaaacgcc cttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    13381 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    13441 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    13501 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    13561 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    13621 tcgcaaccac gggcggcgga ggaggaggtg gcggcgcatc ggcgttaatc gtaaagacga
    13681 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    13741 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    13801 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    13861 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13921 acgttatccg cgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13981 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    14041 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    14101 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    14161 ccgggtcagg aagaacacca gtaccttgtt gtggccactc caccaggcag atggcatcgt
    14221 tggcaaaata atcgcgaatc cccataaact ccagctcctc gggatcggca aggcggtaca
    14281 aatcaaagtg ataaaccatt aaattgtcga gcgtataggg ttcgaccagc gtataagtgg
    14341 ggcttttgac attaccctga tgacccagag cttgtaaaaa gccccggcta aaggtggttt
    14401 tacctgcgcc taaatcgcca tacagataga ttacggttgc gccatcgcag gctttcgcta
    14461 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    14521 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    14581 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgcagc accgtgcgcg
    14641 acacagcccg cacaggcggc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    14701 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    14761 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    14821 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    14881 acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg gcgtgatcac gcgattgtga
    14941 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    15001 cgaaaattct caactttttg cagtgctttt ttcccccact cttgctggcc cagaccggga
    15061 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat cgtcagttca
    15121 tgcaccatca attccggtcg tgcagtcagc agcggcgcaa tgttttcact gcgggtcagt
    15181 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    15241 gccgtgccgt gatcgccacc gataattacc agccgcccgt gatcgccttt atgcgaagtc
    15301 gggcgacgcg gttttagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    15361 tcctgacctg ccagccaact atccagcccc agtgagtcaa aatgcagttg tccggtaaca
    15421 tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa taaaagtgat ggtgtgatcg
    15481 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    15541 accgccgcaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    15601 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    15661 ggccagacaa tattcgaagc atggatctcg cctcccgcgt ttaaccatgc ttcgcgtgcc
    15721 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccagcagcgt gacctcaatg
    15781 ccgaccgctg tggccagccg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    15841 cacagcacca gccaatggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggct
    15901 tcgccagcgc gaagcatcag ctcatagagt gtaagcccca gcgcatctgc cgcctcacgt
    15961 tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg gtatacttac ggggtttttc
    16021 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    16081 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    16141 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    16201 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattgcccgg tacgctgcgc
    16261 gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    16321 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    16381 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    16441 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    16501 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcttt cttcttttta
    16561 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggt
    16621 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    16681 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    16741 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    16801 tggaatcgct attcacaact caccacagaa gaggatttca gcccgcgtaa gccgctacac
    16861 gcaccggaac tcattgagtt attcgcctgg agcgaagaga agtttttaaa agtcacggaa
    16921 ggatcggcga ttcgtcgtat tggtcacctg cgttggctgc gtaatatcgc cgtagcatta
    16981 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    17041 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    17101 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    17161 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    17221 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatctta atttttaact
    17281 tatctatcaa taggttaaaa aaatactatt caccgtgcga agtttctttg aaattaagat
    17341 tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acgaaaacca
    17401 gcaataacgc atcgcatcgc tgtggatatt ttattgagag aagaatttgg agcgggaaac
    17461 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
    17521 cgcttgggtg gtctgtgcct tacagcactt tcaaattttg gagcgggaaa cgagactcga
    17581 actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcgtacta
    17641 cttaattttg ctttcgtaat tttcgcattt ctgcgtcgtt acgggaggcg cattatacga
    17701 gaaatctttc cctctgcaac cccccaaaaa agtttttttt gcgtttttat ctcaagtgct
    17761 gaaattatca gcaagacgtt caatttagcg tcaaaatcac gccgccgcaa gtggattccg
    17821 gcgcttacgt gaccaggaaa aattttacag cttgataaaa tgctcgcggt agtaagccag
    17881 ctccgccacc gattcacgga tatcatccat cgcctgatgc gtcccctgct tggtaaaacc
    17941 atccagaatt tccggcttcc agcggcgcgc cagctctttc agggtgctga catcgagata
    18001 acggtagtgg aagtaggctt ccagctccgg catgtattta aacaggaaac gacggtcctg
    18061 accgatgctg ttaccgcaaa tcggcgattt tcccgcaggc acccactgtt ttaaaaattc
    18121 gagcgttgcc agttcagctt cccgatcgcc catcgtgctc gctttcacgc gctctaccag
    18181 cccgctggcg gtatgggtgc gcacgttcca gtcatccatc agcgccagct gttcatcaga
    18241 ctggtgtact gcaatggtcg gcccttctgc cagaatattc aggttggcat cggtcaccag
    18301 cgtggcaatc tcaataatgc gatcgcgctc gggatccaga ccggtcatct caagatcgat
    18361 ccaaatcagg ttgttttcat tggcactcat gctattttcc acccattcac agtgactata
    18421 ttgatccaca attgcgtgta tcatagatgt tttgcccatc aggggcgacc aggagtctgt
    18481 acgattgagt aaaaataaac tctccaaagg ccagcagcgc cgcgtgaacg ccaatcacca
    18541 gcgtcgtctt aaaacgtcta aggagaagcc cgactacgac gacaatctgt ttggcgagcc
    18601 tgatgaaggt atcgtcatca gccgctttgg tatgcacgct gatgtggaat ccgccgatgg
    18661 cgacgttcac cgctgcaata ttcgccgtac catccgttcg ctggtaaccg gcgaccgcgt
    18721 agtctggcgt ccgggtaaac cggcggcgga aggcgtaaat gtcaaaggga tcgtggaagc
    18781 ggtgcatgag cgtacctcgg tgttgacgcg tccggatttc tacgacggcg taaaacctat
    18841 tgccgccaac atcgaccaga ttgtcattgt ctccgccatt ttgccggagc tgtcgctcaa
    18901 tattatcgac cgttacctgg tggcctgcga aaccttgcag attgagccga ttattgtgct
    18961 caacaagata gacctgctgg acgacgaagg catggcgttc gtcaacgagc agatggatat
    19021 ctaccgcaat atcggttatc gcgtattgat ggtttccagc catactcagg atgggctaaa
    19081 accgctggaa gaggcgttga ccgggcgcat cagcattttt gccgggcagt ctggcgtcgg
    19141 caaatccagc ctgctgaatg cgttgctggg gctgcaaaaa gagatcctga ccaacgatgt
    19201 ctctgacaac tcggggctcg gtcagcacac cacgaccgcc gctcggctgt atcacttccc
    19261 gcacggcggt gatgtgattg attcccctgg tgtgcgtgag ttcggcctct ggcacctgga
    19321 gccggaacaa atcactcagg gctttgtcga attccatgac tatttaggtc tgtgtaaata
    19381 tcgcgattgc aaacacgata ccgatccggg ctgcgctatc cgggaagcgg ttgaggaagg
    19441 gaaaatcgcg gaaacccgtt tcgaaaacta tcaccgtatt ctggaaagca tggcgcaggt
    19501 aaaaacgcgt aaaaactttt ctgatacgga tgactgacaa ttaagctaac cgtcgttaga
    19561 atcgtcccct ttttttcagg atccggcatg ttggccggat caggaacgac aaaacaatgg
    19621 cctggaggct accttgttaa attcatttaa actttcgcta cagtacattc tgccgaaact
    19681 atggcttact cgcctggcgg gttggggcgc aagcaagcgg gcaggatggc tgacaaaact
    19741 ggttatcgat ctgttcgtta aatactacaa ggtcgacatg aaagaggcgc aaaagccgga
    19801 caccgccagc taccgcacct ttaacgaatt ctttgtccgt ccgctgcgtg acgaagtacg
    19861 cccaatcgat accgatccga atgtcctggt catgcctgcc gatggcgtta tcagccagtt
    19921 gggtaaaatc gaagaaaata aaatcctgca agccaaaggc cacaactaca gcctcgaagc
    19981 cctgctggca ggcaactatc tgatggcgga cctgttccgc aacggtacgt ttgtgaccac
    20041 ttacctctcc ccgcgtgact accatcgcgt acacatgccg tgcaacggta ttctgcgtga
    20101 gatgatctac gtgccgggcg atctcttctc cgttaaccat ctcaccgctc agaacgtgcc
    20161 gaatctgttt gcccgtaacg aacgcgtgat ttgcctgttc gataccgaat ttggcccgat
    20221 ggcgcagatt ctggtcgggg cgacgattgt aggcagcatt gagacggtct gggcgggcac
    20281 cattacgccg ccgcgcgaag gtatcatcaa gcgctggacc tggcctgccg gggaaaacga
    20341 cggttctgtg gcactgctga aaggccagga aatgggccgc tttaaactcg gctctaccgt
    20401 tatcaacctg tttgcaccgg gtaaagtgaa tctggttgag caactggaaa gcctgtctgt
    20461 cacgaaaatt ggccagccgc tggcagtatc taccgaaacc tttgttacgc cagacgctga
    20521 acccgcccca cttcctgctg aagagatcga agcagaacac gacgccagcc cattggttga
    20581 cgataaaaaa gaccaggtct aatccatcaa aggaaacgct gacgtgcgcc tgattatcac
    20641 ttttctgatg gcctggtgcc tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa
    20701 acaaatcact caggaactgg agcaggcaaa agcggcgaaa cccgcacagc cggaagtcgt
    20761 agaggcgctc cagtctgcct taaatgcgct tgaggaacga aaaggttccc ttgagcgcat
    20821 caaacaatat cagcaagtta tcgataatta tccgaaactc tccgctactc tgcgcgcaca
    20881 attaaacaac atgcgtgacg agccgcgcag cgtgtcgccg ggaatgtcta ccgacgcgct
    20941 gaatcaggaa attctccagg tcagcagcca gttgctggat aaaagccgtc aggcccagca
    21001 agagcaggag cgcgcccgcg agattgccga ttcgctgaat caactgccgc aacagcaaac
    21061 cgacgcccgc cgtcagttaa atgagatcga gcgccgcctg ggaacgctta ctggcaatac
    21121 cccgctcaat caggcacaaa atttcgcgtt gcagtctgac tccgcacgcc tgaaagcgct
    21181 cgttgatgaa ctggaactgg cgcagctgtc tgccaataac cgtcaggaat tagcgcgctt
    21241 acgctcagag ctggcggaaa aagagagcca gcaactggat gcgtatttgc aggccttacg
    21301 taatcagttg aacagccaac gtcagcttga ggcggagcgg gcgctggaaa gtaccgaatt
    21361 gctggcagaa aacagcgccg atttgccgaa agacatcgtc gcgcaattca aaattaaccg
    21421 cgaactatcg gcggcactga atcaacaggc gcagcggatg gatctcgttg cctcgcaaca
    21481 gcgccaggcc accagccaga cgttacaggt tcggcaggcg ctgaatacgc tgcgtgaaca
    21541 gtcgcaatgg ctgggatcgt ccaacctgct cggcgaagcg ttgcgggcgc aggtggcacg
    21601 gctgccggaa atgccgaaac cacaacagct tgataccgaa atggcgcagt tgcgtgtgca
    21661 acggttacgt tatgaggatc tgcttaataa acagccgctg ctacggcaaa ttcatcaggc
    21721 cgacggtcag ccgctgaccg ccgagcaaaa ccgtattctg gaagcacaac tgcgcactca
    21781 gcgtgagttg ctgaactcat tgttgcaggg tggcgacacg ctactactgg aactgaccaa
    21841 gctgaaagtc tccaacgggc aactggagga tgcgctgaaa gaggtgaacg aagcaacgca
    21901 ccgctatctg ttctggacct ctgacgtgcg cccgatgacc atcgcctggc cgctggaaat
    21961 cgcccaggat ctgcgtcgtc tcatttcgct ggacaccttc agtcagttgg gcaaagccag
    22021 tgtgatgatg ctgaccagca aagagacgat tttgccgctg tttggcgcgt tgattctggt
    22081 cggttgcagt atttactcgc gccgctattt cacccgtttt cttgaacgtt cggcggcgaa
    22141 agtcggcaaa gtgactcagg atcacttctg gctgacgttg cgcactcttt tctggtcgat
    22201 tctcgtcgcg tcaccgctgc cggtgctgtg gatgacgctg ggttacggct tgcgcgaggc
    22261 gtggccttat ccgctggcgg tcgcgattgg cgatggcgta acggccaccg tgccgctgct
    22321 gtgggtagtg atgatttgcg ccacctttgc ccgcccgaac ggcttgttta tcgctcattt
    22381 tggctggccg cgcgaacgtg tttcccgtgg gatgcgctac tacctgatga gcatcgggct
    22441 tattgtgccg ctgattatgg cgctgatgat gttcgataac ctcgacgacc gtgaattctc
    22501 cggttcgctg ggacggcttt gctttatcct catttgcggt gcgctggcgg tggtcaccct
    22561 cagcctgaaa aaggccggga tcccgctgta cctcaacaaa gagggcagcg gcgacaacat
    22621 taccaaccat atgctgtgga acatgatgat tggcgcacca ctggttgcca ttctggcgtc
    22681 agcggtgggt tatctggcaa cggcgcaggc actgttagcg aggcttgaaa cctcggttgc
    22741 catctggttc ctgctactgg tggtttatca cgttatccgc cgctggatgc tgatccagcg
    22801 tcgcaggctg gcgtttgacc gggcgaagca tcgccgggca gagatgttag cgcaacgcgc
    22861 gcgtggcgaa gaggaagcgc atcatcacag tagcccggaa ggggcaattg aagtcgatga
    22921 aagcgaagtc gatctcgatg ccatcagtgc gcaatccttg cggctggtgc gctcaatttt
    22981 gatgttgatc gccctgcttt ctgtcattgt gctgtggtca gaaatccatt ccgctttcgg
    23041 cttcctcgaa aatatttcgc tgtgggatgt cacctccacg gtacagggcg tagaaagtct
    23101 ggagccaatt accctcggtg cggtgctgat tgccattctg gtgtttatca tcaccacgca
    23161 gctggtgcgc aacctgcccg cgctgctgga actggcgatt ttgcagcacc tggatttaac
    23221 gccgggtacg ggctacgcca tcaccaccat caccaaatat ctgctgatgc tgattggcgg
    23281 gctggtcggc ttctcgatga ttggtattga gtggtcgaaa ttgcagtggc tggttgccgc
    23341 gctcggtgtt ggtctcggtt ttggtttgca ggagattttc gccaacttta tctctggcct
    23401 gattatcctg ttcgaaaaac cgattcgcat tggcgatacg gtaacgatcc gcgatcttac
    23461 cggtagcgtg acgaaaatta acacccgcgc caccaccatc agcgactggg accgtaaaga
    23521 gataatcgtg ccgaacaagg cgtttattac cgagcagttt atcaactggt cgctctctga
    23581 ctcggtcacg cgcgtggtgt tgacgatacc ggcccctgcc gatgccaata gcgaagaagt
    23641 gacggaaatc ctgctcaccg cagcgcgtcg ctgctcgctg gtgatcgaca acccggcacc
    23701 ggaagtcttc ctggtggatc tgcaacaggg gattcagatt ttcgagctgc gtatttacgc
    23761 cgctgagatg ggtcaccgta tgccgatacg ccatgagatc caccagctga ttctggcagg
    23821 cttccacgcc cacggtatcg atatgccatt cccgcccttc cagatgcgtc tggaaagcct
    23881 caacggtaag caaacgggga gaacattaac ttctgcgggc aaaggtcgtc aggcgggaag
    23941 tttgtaatgc atgaagctgc caaatgatgt caacgtggct catttagcag cttaacatca
    24001 tcttcagtta tcaccggccc tggagagtat catccatacg cattacttcc tgacaactca
    24061 tcgccggaga gacgatgagt tgcaggattt catatgatca gtcgagagct aactcaggtt
    24121 tattcgccat gcgactgcaa tagttctggt aaatcgccat cgctaacagc gagaagaaga
    24181 cagggccgcc aatcatccac aatgtgctgt cccagtctcc ggcttcaacc acaggttgaa
    24241 tgatggtgaa gacgttcgca aatgtcacca ccagtacgac aaccaccgtc gcaatcattg
    24301 ccgacatacg cgttttgaaa atcacaaatg gtctgtcgag atcctgacgt gctttaaaga
    24361 atgggaaagc cagcgcgagg aacaggtaag gaagcgtcat agacacgttc gccatcagcg
    24421 tcagcttgtt aaagaacgcc gatgcggtac cgccaccaaa cgaaaccagc aggatgaaga
    24481 cagtaaccaa cccgcactgc atccacatgg cgatagaagg catccccatc gcattcaggc
    24541 gcgtcatcgg ttccggccac aacgctttcg gcgtcccctg gatgatggct ttcagcggtg
    24601 aatagcaaag cgtaaagaac gccccggtat aagcgaggaa catcgaaagt ccggtaatac
    24661 gcgcaaacca tacacccagc gacaatgacg cttcaggtga caaatgcagt gcgttaccca
    24721 gcgtcacccc gaggctcttc atcagcacat aggtaatatt gccgaggtta acggaaccat
    24781 tacttaatac ctgctgccag tttgtgctga caccccataa aaatattgcc agcgaataac
    24841 cgattgaaat aacaatagcg gcaaaaacaa tacctttggc aaagttcttt tctggatttt
    24901 ccgttttatc gaccagacca ccaaccgctt caattccgcc ataggcaaaa atagcaaata
    24961 ccacaaacga tagcattgcc agaccggact gataacctgg gttcggtgat gcaaggaaat
    25021 taatatcctg cgcgaaatgc ccaccattta ataacaaaat agtaatgctt actaacagca
    25081 atactaaatt cagacacatt actgcaatac cgcccaccgc agtaatgcgg gcaattttat
    25141 taatcccctt tgaagcaacg acggtgacca gaatcatcca tgctactgcc agcagaccaa
    25201 ccacctgcgt aggctccagt ccggcaatac gccagtgctg ggtcatgtcg ctaccataga
    25261 ggaatgttga gaacggtacc caaactttcg cggaggtact caccatccag atgatataag
    25321 aggaaaacca cataaacgta ccaatgaagg caaaacgtgg tccgacacta ttattcatcc
    25381 aggaatagat accaccttct tctttgcgat aagcagctcc catttcagcc atcattaagg
    25441 cgaatggaat aaagaataac aatgcagaaa atatataaaa gggaatcgca ctataaccca
    25501 ttaagtaata agccgatggg ctattggcaa atccaaatac ggaagtaaag atcatcaata
    25561 tgagtcctat cagactcatc ttttttatcg tgtgaggcat gaaaccatcc ttcatttcag
    25621 cgcgtgtata acacgtgccc actgaactgc actgcgcaac attgagcagg cagcgtaaaa
    25681 accaatacag aattgatacc tggatgatat caaatatcca gtcttaaatt gtggccttta
    25741 acggaaaaat gaaaacatat gctacagaat ggcgcttatc acgccattct tcgctgttaa
    25801 ttcagtaatt tttcagaatt atgcccggtc aacgctaaag gcgatgactt cagccagtgt
    25861 ctccgcgccc agcgccaaca tcaccagacg atcaacacct aatgccacgc cggaacagtc
    25921 aggcataccg actttcaagg cttcaatcag attctggtca atggggtgct gcggcaaacc
    25981 gcgcgccgcg cgcttacggt tatcttgttc aaagcgttgt tgctgctcac gggcatccgt
    26041 caattcatgg aaaccattcg ccagctcaat acctttataa taaacctcaa agcgttcagc
    26101 gacccgatga tcttcggtac tgatttgcgc cagtgatgcc tggctggctg gaaagtggta
    26161 cacaaaggtc ggtttttctt tgccaatatt tggctctacg ccaaaggtaa acagcaattg
    26221 tagcagcgtg tcgcggtctt cttcggtatc agcaacattg ctcaaatcca gtttcgctgc
    26281 gacttcccgc agttgcgttt tgtcggcaga gagcgggtca atttccagat aacgcaagaa
    26341 agcttgttga taagaaaggc tttctgctgc cgggcagtcc agcacctgtt gtaagagatc
    26401 gtccacctcg ttcatcaacc ggtacatatc atagtgcggt cgataccact ccagcatagt
    26461 gaactcaggg ttgtgataac gccccatctc ttcattacgg aagctgcggc acagctggaa
    26521 taccggccca caaccggcaa ccagcaggcg tttcatatgg tattccgggc tggtcattaa
    26581 ccagagattc atcccctgcg aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa
    26641 atgaatatcg gttaccgtcg cctggctcat acaaggcgtc tccacctcca gcactccacg
    26701 atcggcaaag aaacgacgga tctccgccat aatcgccgcg cgttttaata agttaggaat
    26761 ggatgcgctc ggctgccagg atgccgtttc gctcatagtt aaatctccag tttttgacaa
    26821 gggcacgaag tctactcgca acgcgacggc gagacaaatt ttacgcagga atcaaacagc
    26881 ggtgggcagt gactaaaaaa agcacgatct gatggtttag taattaaatt aatcatcttc
    26941 agtgataatt tagccctctt gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat
    27001 ccatcagact atactgttgt acctataaag gagcagtgga atagcgttcg cagaccgtaa
    27061 ctttcaggca cttaccctga agtacggggc tgtgggataa aaacaatctg gaggaatgtc
    27121 gtgcaaacct ttcaagccga tcttgccatt gtaggcgccg gtggcgcggg attacgtgct
    27181 gcaattgctg ccgcgcaggc aaatccaaat gcaaaaatcg cactaatctc aaaagtatac
    27241 ccgatgcgta gccataccgt tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat
    27301 gacagcttcg aatatcactt tcacgataca gtagcgggtg gcgactggtt gtgtgagcag
    27361 gatgtcgtgg attatttcgt ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg
    27421 gggtgcccat ggagccgtcg cccggatggt agcgtcaacg tacgtcgctt cggcggcatg
    27481 aaaatcgagc gcacctggtt cgccgccgat aagaccggct tccatatgct gcacacgctg
    27541 ttccagacct ctctgcaatt cccgcagatc cagcgttttg acgaacattt cgtgctggat
    27601 attctggttg atgatggtca tgttcgcggc gtggtagcaa tgaacatgat ggaaggcacg
    27661 ctggtgcaga tccgtgctaa cgcggtcgtt atggctactg gcggtgcggg tcgcgtttat
    27721 cgttacaaca ccaacggcgg catcgttacc ggtgacggta tgggtatggc gctaagccac
    27781 ggcgttccgc tgcgtgacat ggaattcgtt cagtatcacc caaccggtct gccaggttcc
    27841 ggtatcctga tgaccgaagg ctgccgcggt gaaggcggta ttctggtcaa caaaaatggc
    27901 taccgttatc tgcaagatta cggcatgggc ccggaaactc cgctgggcga gccgaaaaac
    27961 aaatatatgg aactgggtcc acgcgacaaa gtttctcagg ccttctggca cgaatggcgt
    28021 aaaggcaaca ccatctccac gccgcgtggc gatgtggttt atctcgactt gcgtcacctc
    28081 ggcgagaaaa aactgcatga acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt
    28141 ggcgtcgatc cggttaaaga accgattccg gtacgtccga ccgcacacta caccatgggc
    28201 ggtatcgaaa ccgatcagaa ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa
    28261 tgttcctctg ttggtctgca cggtgcaaac cgtctgggtt ctaactccct ggcggaactg
    28321 gtggtcttcg gccgtctggc cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat
    28381 ggcaacgaag cggcaattga agcgcaggca gctggcgttg aacaacgtct gaaagatctg
    28441 gttaaccagg atggcggcga aaactgggcg aagatccgcg acgaaatggg cctggcaatg
    28501 gaagaaggtt gcggtatcta ccgtacgccg gaactgatgc agaaaaccat cgacaagctg
    28561 gcagagctgc aggaacgctt caagcgcgtg cgcatcaccg acacctccag cgtgttcaac
    28621 accgacctgc tctacaccat tgaactgggc cacggtctga acgttgctga atgtatggcg
    28681 cactccgcaa tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc
    28741 accgagcgtg acgacgtcaa cttcctcaaa cacaccctcg ccttccgcga tgctgatggc
    28801 acgactcgcc tggagtacag cgacgtgaag attactacgc tgccgccagc taaacgcgtt
    28861 tacggtggcg aagcggatgc agccgataag gcggaagcag ccaataagaa ggagaaggcg
    28921 aatggctgag atgaaaaacc tgaaaattga ggtggtgcgc tataacccgg aagtcgatac
    28981 cgcaccgcat agcgcattct atgaagtgcc ttatgacgca actacctcat tactggatgc
    29041 gctgggctac atcaaagaca acctggcacc ggacctgagt taccgctggt cctgccgtat
    29101 ggcgatttgt ggttcctgcg gcatgatggt taacaacgtg ccaaaactgg catgtaaaac
    29161 cttcctgcgt gattacaccg acggtatgaa ggttgaagcg ttagctaact tcccgattga
    29221 acgcgatctg gtggtcgata tgacccactt catcgaaagt ctggaagcga tcaaaccgta
    29281 catcatcggc aactcccgca ccgcggatca gggtactaac atccagaccc cggcgcagat
    29341 ggcaaagtat caccagttct ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc
    29401 gcagtttggc ctgaacccag agttcatcgg tccggctgcc attacgctgg cgcatcgtta
    29461 taacgaagat agccgcgacc acggtaagaa ggagcgtatg gcgcagttga acagccagaa
    29521 cggcgtatgg agctgtactt tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga
    29581 tccggctgcg gccattcagc agggcaaagt agaaagttcg aaagactttc ttatcgcgac
    29641 cctgaaacca cgctaaggag tgcaacatga cgactaaacg taaaccgtat gtacggccaa
    29701 tgacgtccac ctggtggaaa aaattgccgt tttatcgctt ttacatgctg cgcgaaggca
    29761 cggcggttcc ggctgtgtgg ttcagcattg aactgatttt cgggctgttt gccctgaaaa
    29821 atggcccgga agcctgggcg ggattcgtcg actttttaca aaacccggtt atcgtgatca
    29881 ttaacctgat cactctggcg gcagctctgc tgcacaccaa aacctggttt gaactggcac
    29941 cgaaagcggc caatatcatt gtaaaagacg aaaaaatggg accagagcca attatcaaaa
    30001 gtctctgggc ggtaactgtg gttgccacca tcgtaatcct gtttgttgcc ctgtactggt
    30061 aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc
    30121 ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg
    30181 ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg
    30241 gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg
    30301 tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaattca cgtgcctgcg
    30361 ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtatc
    30421 gttacaatct aacgtatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
    30481 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct
    30541 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
    30601 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
    30661 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
    30721 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc
    30781 gaggggaaat caagttaagc gatcccacaa caaaatactg gcctgaactt accgctaaac
    30841 agtggaatgg gatcacacta ttacatctcg caacctacac tgctggcggc ctgccattgc
    30901 aggtgccgga tgaggtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
    30961 ctgcatgggc gccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
    31021 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaaca cgtgtcttcc
    31081 agccactcaa actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg
    31141 cctggggata tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaaa
    31201 cttatggtgt gaagtcgacc attgaagata tggcctgctg ggtacgaagc aatatgaatc
    31261 cccgtgatat caacgacaaa acacttcagc aagggataca actggcacaa tctcgctact
    31321 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtaaatc
    31381 ctgacagcat cattaacggc agtggcaata aaattgcact ggcagcacac cccgtaaaag
    31441 cgattacgcc cccaactcct gcagtacgcg catcatgggt acataaaaca ggggcaaccg
    31501 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtata gtgatgctgg
    31561 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc
    31621 tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc
    31681 atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc
    31741 gcagcgacag ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgta
    31801 acagtagtga ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt
    31861 tttgatcacc gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat
    31921 gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag
    31981 agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc
    32041 tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg
    32101 ctggtttgcg gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct
    32161 gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag
    32221 tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc
    32281 aatacgatta acgccagact cgccaggcgc atcgggttag cggactcacc gagcagcaca
    32341 atgccggtga tagccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct
    32401 actggtaacg atttcatcgc ccaggcaagt agcgccatac tgacaatcat cgccgtcacg
    32461 gtaataacac tcggcgtcaa acgactaaag ccgtgggtat atttcaggcc aacggcccat
    32521 accacttcca gcagaccagc aataactaag ataatccagg acatatcagg ctccggagaa
    32581 ttggggccgt ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga
    32641 acggatattt tgccaggtgg caggggaatt tcaactatta ccttagtgac tatcgcagaa
    32701 aaaacaaacg gcacgacata cctgtcgtac cgttattgct tattgctatt gctgcgcttt
    32761 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    32821 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    32881 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    32941 aataatagac aacatcgcca aaaatgacgg aaatctgatg atttttcgga agagaagaaa
    33001 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    33061 cgagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    33121 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    33181 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    33241 tgtacgaaca gcggaacctt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    33301 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    33361 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    33421 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    33481 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    33541 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    33601 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    33661 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    33721 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    33781 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    33841 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    33901 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    33961 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    34021 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccgctgg aagaacagca
    34081 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    34141 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    34201 gggcaacaag cgtaactggc agactgctct tgagtatgtt gctgcgcatc cggaactgga
    34261 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    34321 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    34381 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    34441 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    34501 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    34561 tggtgtgaac gataacgcac aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg
    34621 cgtaatgccg tattacctgc atgtgctcga taaagtacag ggcgcggcgc attttatggt
    34681 gagtgatgac gaagcacggc agattatgcg tgagttgctg acactggtgt cgggttatct
    34741 ggtgccgaaa ctggcgcgag aaatcggcgg cgaacccagc aaaacgccgc tggatctcca
    34801 gctacgccag cagtaactga ttaaacaggg aagttaaaca ttagcctgat gcgctacgct
    34861 tatcaggcct acattgtgca ttgaaattga atggatttgc aatgaactgt aggccggata
    34921 aggcgtttac gccgcatccg gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa
    34981 ctgggaaatt taatctttgc acgttgcata acctttaatt attcatatgc attgctgttg
    35041 ttacttttta tatagctaac accaataatt aacctttctt ttctgtttat atagagtgag
    35101 cacggctatt tatgttattt taacaactat tactaccaag cccatttatc tggcttataa
    35161 attaaaatta attatttaat tgagaaatgg ttagggagaa cctacatggc cataagcatc
    35221 aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact ggcgctgaaa
    35281 attaaagaac ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga actgcgcgac
    35341 ctgctgattg cgctggaaag tcgtatgcat caaaagcata aactggatgc tgctgcacgc
    35401 cttcagtatg agcaagctcg cgataaagtc attaaaaaaa tggcagaaaa catcccagaa
    35461 attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac gctggaactt
    35521 accgataatc aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg tagcaaatgt
    35581 gagttagtcg tcaatgagtt gcagattgaa atgctggcgc gagcaatcat tcatgcaatt
    35641 aataatgcgg aaatgcgtga actggcatta cgtattacct cactgttaga tttcctgccg
    35701 ttatatgacg ttgactgcca ggaaaatggc aatctggaat atgacaccta ttcccaaccg
    35761 gaatggaaac ataacctgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa
    35821 agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa
    35881 gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg
    35941 ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc
    36001 caggatcaat gcttacgcgc gctgcatcac ttacgtgttc aatccaccag caaaactgca
    36061 ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg
    36121 actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa
    36181 attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca
    36241 ggcaatgata cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct
    36301 gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc
    36361 tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg
    36421 cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac
    36481 ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac
    36541 tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac
    36601 cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg
    36661 ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc
    36721 agacatttct gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc
    36781 gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcattttt
    36841 cggcaggtcg gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta
    36901 ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga tcatgttgcc
    36961 gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc
    37021 aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc
    37081 acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag
    37141 tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc
    37201 agcacgggtc gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc
    37261 ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgttcctg
    37321 cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
    37381 acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt
    37441 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
    37501 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
    37561 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
    37621 gttaacaacc agagttgcca gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
    37681 tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tagagatttt
    37741 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
    37801 gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc
    37861 gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat
    37921 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg
    37981 agcaatcgct ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc
    38041 ggtaacggct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
    38101 tttcagaccc tcagtgatga tagcctgagc cagtacagtt gcagtggtgg taccgtcgcc
    38161 tgcagcgtcg ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
    38221 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
    38281 accgaaagat ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc
    38341 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
    38401 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt
    38461 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
    38521 tcttctcaga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat
    38581 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
    38641 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
    38701 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
    38761 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
    38821 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
    38881 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct
    38941 tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa
    39001 aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc
    39061 caaggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
    39121 ttagctgcgc tggtagcggg caataacagc ctgtgggcgt ggcccgtttt gattatctta
    39181 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggt
    39241 gtcgcgcact tcgtcggtat ggcgtttggt tcgcgacttg agcgagtcac cggctggttg
    39301 tttttatcgg tcattcccgt gggtttgcct gccgcgctac aaattgccgc cgggttcggc
    39361 caggcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg
    39421 ctggtgtggt atatcggtac tcgcggtgcc agttccagtg ctaatctaca aaccgttatt
    39481 gccggactta tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg
    39541 aatatcccct tcccggcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca
    39601 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
    39661 aatccagagc gtgattttcc tcgcgcattg atgattggtc tgctgctggc aggattagtc
    39721 tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg
    39781 gcagcatcgc ttccaaaaat tgtagtgcag ttgttcggtg taggagcgtt atggattgcc
    39841 tgcgtgattg gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc
    39901 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
    39961 cgccatatcc cgaataatgc cctcaatgcg gtgctcggct gctgtgtggt gagcacgttg
    40021 gtgattcatg ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
    40081 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga
    40141 ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa
    40201 agtctctatg cgctgatcat gctggcgggg ttatggctgt tgctgccaaa acgaaaaacg
    40261 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
    40321 atgatcaagg cggtcgcgct catcatcctt tcgctggtac tcaccatcaa aagtattacc
    40381 gccaccggtc ccggcgctaa aaccgccgcc tggcatacga gaaaagcgca aatgcggcat
    40441 caacttcact gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc
    40501 ggtgaaaaag cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat
    40561 catctccgcc gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
    40621 aaagccctgg ttacgtacca gtgacatccc gataactgac gtgaatataa ccagcacgag
    40681 ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct caatatagac
    40741 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
    40801 gtaaaacccg gctacctgaa tgggttgcga atcgcgttca gcttatattg tggtcattag
    40861 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt
    40921 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga
    40981 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
    41041 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
    41101 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
    41161 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
    41221 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
    41281 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
    41341 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
    41401 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
    41461 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
    41521 catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt
    41581 tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
    41641 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
    41701 ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa
    41761 aatatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
    41821 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
    41881 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct
    41941 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
    42001 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
    42061 ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
    42121 aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
    42181 ggtcagctgc agttgaacgt tatggagccg gtcattggcc aggccatgtt cgaatccgtt
    42241 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
    42301 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
    42361 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
    42421 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
    42481 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
    42541 gaacagtaat cgtacagggt agtacaaata aagaaggcac gtcagatgac gtgccttttt
    42601 tcttgtgagc agtaacttaa aaataacaac ctaatatcaa cttgttaaaa aacaaggaag
    42661 gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga
    42721 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
    42781 ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc
    42841 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
    42901 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
    42961 actatctttg ctggtactgg caacatatct ctggcgacac tgccagttat cgctgaagtt
    43021 gcgaaggaac aaggcgtcaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag
    43081 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
    43141 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
    43201 gttctggtga tgtcctttct ggtcactatg ctattcaact ccaaactctc tgacgatccg
    43261 atttatcgca agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa
    43321 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
    43381 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc
    43441 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
    43501 gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt
    43561 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
    43621 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
    43681 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggcc
    43741 ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg
    43801 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtaca
    43861 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
    43921 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg
    43981 gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata
    44041 gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
    44101 ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga
    44161 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
    44221 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
    44281 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
    44341 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
    44401 gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta
    44461 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttac ttttgatttt
    44521 cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac
    44581 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccacaa
    44641 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
    44701 cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc
    44761 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
    44821 gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg
    44881 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
    44941 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc
    45001 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac
    45061 acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac
    45121 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
    45181 ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc
    45241 caacagggcg gctcacctgg cggtgtgttt gttatggggg cgattgccgg actgatctgt
    45301 tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
    45361 atgtggctgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg
    45421 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
    45481 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
    45541 ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc
    45601 tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
    45661 gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc
    45721 gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
    45781 gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc
    45841 gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca
    45901 gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta
    45961 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
    46021 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
    46081 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
    46141 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
    46201 ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca
    46261 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
    46321 cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagttgct ggcacgttat
    46381 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc
    46441 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
    46501 gcggcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
    46561 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
    46621 agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
    46681 tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat
    46741 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag atta
//
LOCUS       DAZGCS010000026        45604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGCS010000026 DAZGCS010000000
VERSION     DAZGCS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.026"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHK01_001551"
     CDS             complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHK01_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO8096522.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHK01_001552"
     CDS             1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHK01_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO8096523.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHK01_001553"
     CDS             1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHK01_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO8096524.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHK01_001554"
     CDS             complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHK01_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO8096525.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2576..3472
                     /gene="xerD"
                     /locus_tag="ACHK01_001555"
     CDS             2576..3472
                     /gene="xerD"
                     /locus_tag="ACHK01_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO8096526.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHK01_001556"
     CDS             3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHK01_001556"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO8096527.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4213..5946
                     /gene="recJ"
                     /locus_tag="ACHK01_001557"
     CDS             4213..5946
                     /gene="recJ"
                     /locus_tag="ACHK01_001557"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO8096528.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6037..7135
                     /gene="prfB"
                     /locus_tag="ACHK01_001558"
     CDS             join(6037..6111,6113..7135)
                     /gene="prfB"
                     /locus_tag="ACHK01_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO8096529.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7145..8662
                     /gene="lysS"
                     /locus_tag="ACHK01_001559"
     CDS             7145..8662
                     /gene="lysS"
                     /locus_tag="ACHK01_001559"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8096530.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHK01_001560"
     CDS             complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHK01_001560"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO8096531.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            9308..9379
                     /locus_tag="ACHK01_001561"
     CDS             9308..9379
                     /locus_tag="ACHK01_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096532.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHK01_001562"
     CDS             complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHK01_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO8096533.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHK01_001563"
     CDS             complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHK01_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO8096534.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            11387..13306
                     /gene="ygfT"
                     /locus_tag="ACHK01_001564"
     CDS             11387..13306
                     /gene="ygfT"
                     /locus_tag="ACHK01_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO8096535.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            13306..13794
                     /gene="ygfS"
                     /locus_tag="ACHK01_001565"
     CDS             13306..13794
                     /gene="ygfS"
                     /locus_tag="ACHK01_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8096536.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13830..15197)
                     /gene="ghxQ"
                     /locus_tag="ACHK01_001566"
                     /gene_synonym="ygfQ"
     CDS             complement(13830..15197)
                     /gene="ghxQ"
                     /locus_tag="ACHK01_001566"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO8096537.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(15233..16549)
                     /gene="guaD"
                     /locus_tag="ACHK01_001567"
     CDS             complement(15233..16549)
                     /gene="guaD"
                     /locus_tag="ACHK01_001567"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO8096538.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(16567..17967)
                     /gene="xanQ"
                     /locus_tag="ACHK01_001568"
     CDS             complement(16567..17967)
                     /gene="xanQ"
                     /locus_tag="ACHK01_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO8096539.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(18132..21002)
                     /gene="xdhD"
                     /locus_tag="ACHK01_001569"
     CDS             complement(18132..21002)
                     /gene="xdhD"
                     /locus_tag="ACHK01_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO8096540.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(20999..21778)
                     /gene="ygfM"
                     /locus_tag="ACHK01_001570"
     CDS             complement(20999..21778)
                     /gene="ygfM"
                     /locus_tag="ACHK01_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO8096541.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(21829..23157)
                     /gene="ssnA"
                     /locus_tag="ACHK01_001571"
     CDS             complement(21829..23157)
                     /gene="ssnA"
                     /locus_tag="ACHK01_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO8096542.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(23160..26258)
                     /gene="ygfK"
                     /locus_tag="ACHK01_001572"
     CDS             complement(23160..26258)
                     /gene="ygfK"
                     /locus_tag="ACHK01_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO8096543.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(26580..27158)
                     /gene="mocA"
                     /locus_tag="ACHK01_001573"
     CDS             complement(26580..27158)
                     /gene="mocA"
                     /locus_tag="ACHK01_001573"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO8096544.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            27261..28031
                     /gene="yqeC"
                     /locus_tag="ACHK01_001574"
     CDS             27261..28031
                     /gene="yqeC"
                     /locus_tag="ACHK01_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO8096545.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            28079..29704
                     /gene="yqeB"
                     /locus_tag="ACHK01_001575"
     CDS             28079..29704
                     /gene="yqeB"
                     /locus_tag="ACHK01_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO8096546.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(29745..30677)
                     /gene="arcC"
                     /locus_tag="ACHK01_001576"
     CDS             complement(29745..30677)
                     /gene="arcC"
                     /locus_tag="ACHK01_001576"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8096547.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(30725..32110)
                     /gene="hyuA"
                     /locus_tag="ACHK01_001577"
     CDS             complement(30725..32110)
                     /gene="hyuA"
                     /locus_tag="ACHK01_001577"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO8096548.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(32163..33374)
                     /gene="ygeY"
                     /locus_tag="ACHK01_001578"
     CDS             complement(32163..33374)
                     /gene="ygeY"
                     /locus_tag="ACHK01_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO8096549.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(33432..34628)
                     /gene="dpaL"
                     /locus_tag="ACHK01_001579"
     CDS             complement(33432..34628)
                     /gene="dpaL"
                     /locus_tag="ACHK01_001579"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO8096550.1"
                     /translation="MSVFSLKIDIADNKFFNGETSSLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(34686..35873)
                     /gene="ygeW"
                     /locus_tag="ACHK01_001580"
     CDS             complement(34686..35873)
                     /gene="ygeW"
                     /locus_tag="ACHK01_001580"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO8096551.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            36352..38124
                     /gene="ygeV"
                     /locus_tag="ACHK01_001581"
     CDS             36352..38124
                     /gene="ygeV"
                     /locus_tag="ACHK01_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO8096552.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQVEIVDENLCRVAGTG
                     AYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVI
                     LQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIF
                     ATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGH
                     MQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRV
                     MRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQL
                     LESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREI
                     LPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIE
                     LLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVI
                     DSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQ
                     VADELGIGIATLYRKIKKYELLNT"
     gene            complement(38164..38643)
                     /gene="xdhC"
                     /locus_tag="ACHK01_001582"
     CDS             complement(38164..38643)
                     /gene="xdhC"
                     /locus_tag="ACHK01_001582"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO8096553.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(38640..39518)
                     /gene="xdhB"
                     /locus_tag="ACHK01_001583"
     CDS             complement(38640..39518)
                     /gene="xdhB"
                     /locus_tag="ACHK01_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO8096554.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(39529..41826)
                     /gene="xdhA"
                     /locus_tag="ACHK01_001584"
     CDS             complement(39529..41826)
                     /gene="xdhA"
                     /locus_tag="ACHK01_001584"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO8096555.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            42241..42996
                     /gene="actS"
                     /locus_tag="ACHK01_001585"
     CDS             42241..42996
                     /gene="actS"
                     /locus_tag="ACHK01_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO8096556.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            43075..43148
                     /locus_tag="ACHK01_001586"
     tRNA            43075..43148
                     /locus_tag="ACHK01_001586"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43107..43109,aa:Gly,seq:ccc)
     gene            43382..44992
                     /locus_tag="ACHK01_001587"
     CDS             43382..44992
                     /locus_tag="ACHK01_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO8096557.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            45005..45124
                     /locus_tag="ACHK01_001588"
                     /pseudo
     CDS             45005..45124
                     /locus_tag="ACHK01_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            45084..45604
                     /locus_tag="ACHK01_001589"
                     /pseudo
     CDS             45084..45604
                     /locus_tag="ACHK01_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        11410 a        11172 c        11795 g        11227 t
ORIGIN      
        1 taaatgcctg atgcgataaa gagatttact cttcgagcga atacggcagc ggctcgatat
       61 gcagtgtatt cgcatcgtcg cgtacgcgga agatgctatc cggttccata tcgttattca
      121 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
      181 tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc
      241 tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat tttgctcgcg
      301 ccaccatctc ttgtccggta tagcagcctt ttttaaagct gatgccgccc agcgcctgga
      361 ggttggtcgc ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
      421 cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt
      481 tatcggtcag catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt
      541 gttcaaacca tagcaaagtg gtcgcgcctt ctttaactac ctgtttttct ttcgaaggca
      601 gttcactaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca
      661 cacgctcgtc gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag
      721 tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga
      781 acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct
      841 gatcttctgc catctggctg acatctgctg tcacctgacc ctgcatatat ttttcgctgt
      901 ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac
      961 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
     1021 ttgcttaacg gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt
     1081 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc attgaattgt
     1141 tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaaggcgaaa
     1201 aacacgttac aatagccggg tactggattt tcgtgagaca ggaagaagat ggacattaac
     1261 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
     1321 atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt
     1381 cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg
     1441 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
     1501 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca
     1561 tgggctggtt gccgctgtta ttttactcat gccctggcca ctcagttaca ccccgttatg
     1621 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gtattaatgc
     1681 tcgccagggg gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
     1741 gtgcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
     1801 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
     1861 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
     1921 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
     1981 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
     2041 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
     2101 ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac
     2161 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
     2221 caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct
     2281 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
     2341 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
     2401 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
     2461 tcataacgta agcttctcga ttctgctgtg gcttttgcag ctagtgtatc agaatcgctt
     2521 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
     2581 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
     2641 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
     2701 ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg
     2761 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
     2821 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
     2881 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg aacgtttatt
     2941 acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
     3001 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
     3061 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg taccgttagg
     3121 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
     3181 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
     3241 cttctggcac cgcattaaac attatgctgt gctggcgggt atcgacagcg aaaagctgtc
     3301 accgcatgta ttgcgtcacg cttttgccac ccatttatta aatcatggtg cggatttacg
     3361 cgtggtgcag atgctactgg gccacagcga cctctccacc acgcaaattt atacgcatgt
     3421 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
     3481 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
     3541 aggttttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
     3601 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
     3661 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
     3721 cggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
     3781 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
     3841 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg
     3901 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
     3961 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
     4021 taaaagtgcc gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt
     4081 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg
     4141 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
     4201 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
     4261 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggcgtgcgc
     4321 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggt
     4381 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
     4441 gtcggtgatt tcgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
     4501 tcgcttggtt gcagcaatat cgactacctg gtaccaaacc gtttcgaaga cggttacggc
     4561 ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
     4621 gataacggta tttcctccca tgcgggggtt gtacacgctc gctcgttggg cattccggtt
     4681 attgttaccg atcaccattt gccgggcgaa acattacccg cagcggaagc gatcattaac
     4741 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
     4801 tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgtggc
     4861 atcgcaattc ctaacctggc agaactgctg gatctggtcg cgctgggaac agtggcggac
     4921 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
     4981 gccggaaagt gccgtccagg gattaaagcg ctgctggaag tggcaaaccg tgatgcacaa
     5041 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccggacga
     5101 ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg acaacatcgg cgaagcgcgc
     5161 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
     5221 caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa gtcgcgacac gctacccggc
     5281 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
     5341 atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga tggtacgctg
     5401 aaaggttcag gtcgctccat tcaggggctg catatgcgtg atgcactgga gcgattagac
     5461 acactctacc ctggcatgat gctgaagttt ggcggtcatg cgatggcggc gggtttgtcg
     5521 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tactgagtgg
     5581 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa
     5641 atgaccatgg aagtggcgca gctgctgcgc gatgctggcc cgtgggggca gatgttcccg
     5701 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
     5761 ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat
     5821 gtcgataccg ctctctggcc ggataacggc gtgcgcgaag tgcaactggc ttacaagctc
     5881 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
     5941 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
     6001 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
     6061 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
     6121 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
     6181 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
     6241 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
     6301 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
     6361 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
     6421 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
     6481 tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga
     6541 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
     6601 ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaga gcccgtttga
     6661 ctccggcggt cgtcgccaca cgtcgttcag ctctgcgttt gtttacccgg aagttgatga
     6721 tgatattgat atcgaaatca acccggcgga tttgcgcatt gacgtttatc gcgcgtccgg
     6781 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccgac
     6841 cgggatcgtg acccagtgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
     6901 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
     6961 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
     7021 tgatgactcc cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt
     7081 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
     7141 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
     7201 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
     7261 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga
     7321 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
     7381 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt
     7441 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
     7501 cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
     7561 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
     7621 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
     7681 atcccgtaac acctttaaag tacgctcgca gatcctctct ggtattcgcc agttcatggt
     7741 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgataccag gcggtgccgc
     7801 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
     7861 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
     7921 ccgtaacttc cgtaacgaag gtatttccgt acgtcataac ccagagttca ccatgatgga
     7981 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
     8041 tactctggca caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga
     8101 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
     8161 aaccgacatg gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg
     8221 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
     8281 ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
     8341 gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg
     8401 tggtcgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg
     8461 cttcctggat caggttgccg cgaaagacgc aggtgacgac gaagcgatgt tctacgatga
     8521 agattacgtt accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
     8581 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
     8641 ggcgatgcgt ccggtaaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
     8701 ttttttaatt gtgctgcgcg aaagcagaca acaattttct tgcttcacta ttggctgcct
     8761 gcatcaccat ccacggactg aacgcccacg gtgtggcatc aataccgtgt aatacatctg
     8821 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
     8881 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccattcgga tcggtggcgc
     8941 gatagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
     9001 ggcaacggcg gatcaccgcg tcttcgttgc tttctcccag ttgtgggtgc ccacaaaccg
     9061 agttagtcca cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt
     9121 gtcctttggc attaaacagc caactggaga acgcgagatg taagcgggtg tctgccgtgt
     9181 gtgcggcata cttttccagc gtaccagtgg gaactccctg tgcattcaat aaaatgacgt
     9241 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
     9301 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgccg
     9361 acaggtaaaa accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
     9421 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
     9481 agcgcgcatt aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca
     9541 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
     9601 ggtcagcatg atgctgatag ccattaaaga agacattcag cacaacggcg ctaagggttg
     9661 cgagcataat gccgctatgc agcaacggtt gcagtacggc cggtaactta gaaaagaaat
     9721 cgtgagagag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
     9781 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
     9841 cgaacatcac cagcccagcg ccgcccagca caaattgcgg aatggaggct accagcaccg
     9901 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
     9961 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg agaaaatgac gtgtggggga
    10021 agctgttaaa cgtgccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    10081 tatcgtgtga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    10141 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    10201 tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca cgaggcatca tgtagcccgg
    10261 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    10321 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    10381 cggcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    10441 cggcccagtc aatacccacc tgaatgatgc taagcccaat agaagtaata accacaccgg
    10501 taaccagtgg cgggaataaa ggcatcaagc gaccaataag tggcgctaat aatgtggtga
    10561 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    10621 caatggctat cattggtgtt acagcagcaa aggtcaccga cataatcacc ggcaggcgga
    10681 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    10741 gatccgagct aatgagcatc gcaatagctt cttttgagag gcccagtcgg tcaccaatca
    10801 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    10861 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    10921 atgagggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    10981 aaaagcaacc gtttacgtta atgaaactgt ctaaacacat gctcgtggag tgccagtttt
    11041 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtgcttaaac
    11101 cacgaaaatg agaagagaac ctgacaatga aattatctgt aattttctca tattgatatt
    11161 tcattaatca ttctggcatg tcgcactctc gcatttaatc gtttttatct ggatagcgct
    11221 cttttggtcg gcaatgttga aataatgaaa gcggcataga ttgaggggca ataatttgaa
    11281 ctcagtcgca aaattataat taaaacggtc atgctaccta atatatgccc gtttattgca
    11341 tcgcgaaatt tgagaagaca taactcatta aatgaaaggg atgcaaatga ataagtttat
    11401 cgctgctgaa gctgcggaat gtataggctg tcatgcttgt gaaattgcct gtgcggtggc
    11461 acacaatcaa gaaaactggc cgctgagtca cagtgacttt cgaccgcgta tccacgttgt
    11521 agggaaaggc caggctgcga atccggtggc ctgccatcac tgcaacaatg ccccttgcgt
    11581 tacggcttgt ccggttaatg ctctgacttt ccagtccgat agcgtacaac tggacgagca
    11641 aaaatgtatt ggctgtaaaa gatgtgcaat cgcttgcccc tttggcgtcg ttgagatggt
    11701 cgatacgatt gcgcagaaat gcgacctttg taaccagcgc agttccggca cgcaagcctg
    11761 tattgaagtc tgcccaacac aggcgttacg actgatggac gataaagggt tacagtggat
    11821 aaaggtggct cgccagcgca aaacggcagc aggaaaagcg tcatcagatg ctcagccatc
    11881 tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc gcagataaaa tttcagcgag
    11941 tgaacggaaa acccactttg gcgaaatata ttgcgggctg gatccactgc aagcgactta
    12001 tgagagtgac cgctgtgttt attgtgccga aaaagctaac tgcaactggc attgcccgct
    12061 gcataacgct attccggatt acatccgtct ggtacaggaa ggaaagatta ttgaagcggc
    12121 agaactttgc caccagacca gttccttacc agaaatttgc ggcagggtat gtccacagga
    12181 ccgtctttgt gaaggcgcat gtactttgaa agatcactcg ggcgcagtct ctatcggtaa
    12241 tctggaacgc tacatcaccg ataccgcgct ggcgatgggc tggcgtcccg atgtcagcaa
    12301 agttgttccc cgtagcgaaa aagtggcggt gattggcgct gggccagcag ggttagggtg
    12361 tgctgatatt ctggcgcgcg cgggagttca ggttgatgtc tttgatcgcc atccagaaat
    12421 tggcggtatg ctgacttttg gcattcctcc tttcaaactc gataaaacgg tattaagcca
    12481 gcggcgagag atattcaccg caatgggaat cgactttcat cttaactgtg aaattggccg
    12541 cgatattacc tttagcgatt taacttctga atatgatgca gttttcatcg gcgtggggac
    12601 ttacgggatg atgcgagcag atctgccgca tgaagatgcg cccggtgtca ttcaggctct
    12661 accgttcctg actgcccata cccgccagct catgggattg ccggagtctg aagagtatcc
    12721 gctgacggac gtggaaggta agcgagtcgt ggtattgggc ggtggcgata cgacaatgga
    12781 ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg acctgcgcgt atcgtcgtga
    12841 tgaagtcagt atgccgggct cgcgcaaaga ggtggttaat gcgcgcgagg aaggtgtcga
    12901 gtttcagttc aatgttcaac cgcaatatat cgcttgtgac gaagatgggc gtttaactgc
    12961 ggtgggcctg attcgtaccg ccatgggtga gccggggccg gatggtcgcc gtcgtcctcg
    13021 tccggtagcg ggttcagagt ttgaattgcc cgccgatgtt ctgattatgg cctttggttt
    13081 ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt aaactcgata aatggggcct
    13141 gattcaaacc ggtgacgtcg ggtatttacc tacccagacg catctgaaaa aagtctttgc
    13201 tggtggtgat gcagttcatg gcgcggatct ggttgtcact gcaatggccg caggaaggca
    13261 ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca tcgtgatgaa atcgttaatt
    13321 atcgttaatc cggctgactg tattggctgc cgtacttgtg aagtagcctg tgtggtcgct
    13381 cacccttcag aacaggagtt gaatgccgat gtctttttac cccggctgaa ggtacagcga
    13441 ctggacagca tcagtgcgcc ggtgatgtgc catcagtgtg aaaacgcccc ttgtgttggc
    13501 gcttgccccg tgggggcgct gacgatgggc gagcaggtgg tgcagaccaa ttctgcccgt
    13561 tgtattggct gtcagagttg cgtcagtgcg tgcccgtttg gaatgatcac cattcagtcg
    13621 ttgccggggg atacccgaca acaaattgtg aaatgcgatc tctgtgaaca gcgggaagag
    13681 gggccagcct gtgttgaatc ttgcccgaca caggcgctgc agttgcttac tgaaagagaa
    13741 ctcaggcgag tccgccagca gcgtattgtt gccagcggtg agaatcctct gtgaaagaaa
    13801 tctccgcgct gaaaggcgcg gagaatctat tagattgccc aaccacccgc gtaaaatgcg
    13861 accagtgcgg cagtaatgat caccgtacca atattcaact tttgccattc gcgagcaaag
    13921 acacggccta cgaccagtgt cacaaagccc agcataatac cggtaacgat attacaagtc
    13981 agaacgatga acacggcaca caccaggcca gccattgcgt caataaaatc gttgaaatcc
    14041 agcttcgaga cgttacttaa catcagcaaa cccacgtaca tcagagcggg tgcagtggcg
    14101 taaccaggga tcaaaaatga cagcggtgat aagaacagaa tcagcaggaa taacgccccc
    14161 actacggttg cggtcaaccc tgttttaccc ccggcggcgg ttcctgccgc tgattcgata
    14221 taaaccgctg cgggcgctgc gcccaccaga ccggagaata ttgaacttac tgagtcactg
    14281 gtcagggctt tgccgccatt gatgatctgg ttgtctttat ccaacaaatt cgcctgaccg
    14341 gcgacggcac ggatggtgcc agtggcgtcg aacactgcgg tcatcaccaa tgccagtaca
    14401 ctcggaagta cggttggctg gagtgcgccc ataatatcga ggctgaaaat cagagactta
    14461 ccatcttcgc cagtcagact tggcatcgcc accagaccgt ggtatttcac cgctgggtca
    14521 aagattaagc cgataatcga aattgcgata atcaccaaca agatcccgcc gggtacgcgg
    14581 cacttctcca gaccgaagat gaccgccagc cccagcaagc tcatcatcac cgggaaggag
    14641 gtaaacgcac cgagcgccac tggcaagcct tcaatcgggt ttttgataac catacccaca
    14701 ccgttagcag caatcagcag cagaaacagg ccgataccga tacctgtacc gtgagcgata
    14761 cccatcggta aattacgtaa gatccaggta cgcacaccgg ttacggaaat agcggtgaag
    14821 atgacgccca tcagaaagac cgcgcccagt gcgacaggaa cgctaatttg ttgcccgagt
    14881 accagactga atgcggtaaa tgccgtcaag gaaatcgcac aaccaatcgc cattggcaaa
    14941 ttggcccaca accccatcag caacgagccg aagcccgcga ccagacaggt ggcaacaaac
    15001 acagctgcgg gaggaaaacc tgctttgccc agcattcccg gaacgacgat aacggaataa
    15061 accatggcca gaaaggtcgt taagccagcc agtacttcct gacgaacggt actgccacga
    15121 gcggtaattt taaaataatt atccagcgcg ccctgtggct ttggtgcgtc cggtgtttgt
    15181 aggatgtctc cagacataat gatgtcctca cgaggatgtt aaagttttat tattagttgc
    15241 gttcgtacac cagacgacca tcaacgtagg tgcggtagat cgaacggtca tcgcccaacg
    15301 tcatcatcac gaacaatttg tcgactaaag aaacagagtt gtcatagcgc agctgctgta
    15361 gcggagtggc ggtgggttcc atcaccacga aatcagcctc tttgccaggt aaaaagttgc
    15421 caatcaaatc gtcaaggccc agagatttcg ctccgccgag cgtggccagg taaaacgctt
    15481 catatgccga gaggcgatag ccttgtaatt gcaatacttt gtaggcttcg ttcagcgttt
    15541 gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc catgcccact ttaactttct
    15601 tctgccatgc ttttttcaag ttgaataagc cgctgccgag gtaaaggttg gaggtcggac
    15661 agaaagcaat gctggatttg gtttcgctga gacgatccca ctctttttct tcgagatgga
    15721 cgcagtgagc aaagacacag tttttaccgg tcaggccgta ctgatggtaa acatccagat
    15781 aaccatcatg gtcaggataa agcgatttca cccaggcaat ttcatctttg ttttcacaga
    15841 gatgggtatg tacccacgta tccggatatt cttctttcag gcgttgcgcc atcgccatct
    15901 gttcaggaga tgaggtcggg gcgaagcgtg gcgtaatcgc atatagcaga cgaccatttt
    15961 tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct gctttcggca gtgtcgagca
    16021 gataatccgg tgcgttgcgg tccatcatca ccttaccggc aatcatacgc atattgatat
    16081 gactggcggc ttcaaacagc gcatcaacag attgcggatg aacagtgcca aacaccagcg
    16141 ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa tgccgacatt tcgcgggcgt
    16201 actctaaatc ctcataacga cgttcagtag ggaaggtgtg tttattcaac cactccagca
    16261 attgttcacc ataggccccc accatttcac tttgcggata atggatatgt gtatcgacaa
    16321 agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg aatagtgtca ggaatttgat
    16381 gctttccgtc ttcccattcg ccaaaccatt ccacttttcc ctgtttaatg agtaataaac
    16441 catcctcaat aaaccgcagc gcagaggcaa tctcttccgg gttatcgacc gtacgggtga
    16501 catcaataaa actgcctcgt accgctttca acgtgtgttc tcctgacatc attgactcct
    16561 ttactgttaa tccatctctt ccgctgaggt aataccaggc agaacgtttt cctgtcggta
    16621 gccaccaggg aggataatat ttaataaaat cgcagttaac ccgccagcac aaatagggtt
    16681 ttcaactagt acataaatag aggctggcaa tattttaaaa atttcgggat cgtaggagac
    16741 gccaagtcct aaacctaaag aagtggcgac aataagtgtt tctcggcgct ttaaaccgtt
    16801 ggtgatgatt atgcgaatcc cagcgatggc aatcatggaa aacatcaacg tcattgcgcc
    16861 tcccagaact gccgagggaa tggtcgtgaa gaagccgcca atcatcggaa acaaaccgag
    16921 gataaccagc attaccgcga tggttcgccc gacataacgt gaagcgacgc cagtcatctg
    16981 aataacccca ttattttgcg caaacgtggt taagggtaat gacccgacag cggaggcgat
    17041 aacagaaacc agaccgtctg ccagcacgcc gcctttcagt cgggactgat actcttctcc
    17101 ctgaatgggt cggcgggaaa ccattgccgt ggcggtgata tcgccgacag cttccagcac
    17161 gctaagcaga taaatcgtgc caaccaccag gaactgatgg aagctaaaac taaagccgta
    17221 tttgaacgga tgcgggatgg tgattaacgg caaattgcgc atactgctga aatccaccat
    17281 gcccaggcat aacgatgcga tatagccgac acatagccca atggcgatcc ctcccatgcg
    17341 tagcaacgga ctgcgacagc agttaaagcc gatcaccaca attaaaacca ataaaccaac
    17401 gccgagatgt tcgtaattgc cgaatgtacc gctgcttttg gctgcaaatc ctccaccaaa
    17461 atcgataatg ccgactttaa tcaggcttaa gccgatcatc agtaccacaa taccgctgac
    17521 ggtaggcgta ataacccgac gtaaataggg caagataaat gaagatccga caaccagaaa
    17581 tgcgccaacg aaggagacgc cgagaagcga cgacatgatt aactcttcgt gaaaaccgtc
    17641 gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca aatgaaaaat tgactgactg
    17701 aattgagagt aggccagaac cgacgatgcc gtagcggttt acttgtaacc aggtaccaat
    17761 accagaggcg atcatcgcca tagaaacaag ataggcagtt gtttcagcgg aaagctgtaa
    17821 ggccgccccc acgattaacg cgggggttac catcggaacg aaaattgcca acagatgggt
    17881 aatggcacca acgagagcct gatgaaaggg agggcgatcc tccagttcaa atataaggtc
    17941 agaacctgca ttgtttatat cagacatccc ttcactcctt ttaaagtaaa tttgatcatc
    18001 tgaattagta aaataaattc ccacgaattt ggggcgcagg acgccttgag aacggcttat
    18061 ctcattttcg cagtcactat ttagtcatcg tccaaacaga gaaacaggcc gccgaagcgg
    18121 ccatttatta tttatatttt ttccagcgca gtgagtattt tctccggtgt gaaatgccat
    18181 tcgcgtaacc agatgccgca tgcatcgtga attgcggtag caatcgccgg agctgcgcca
    18241 tttacaccga tttccgagat cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc
    18301 accagcacag cgcggaaatc acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt
    18361 aaatcacgcg ttaacgggtg accttcggcg tcataaatga tctcttcgct catactgtgg
    18421 ccgatagctc gcagggtagc accgtagatt tgtcccaatg ctaattccgg gttgaccggt
    18481 gtaccgcagt ccagcaaggc gtagaattta tccaggcgga tttcacccgt acgcgtgttg
    18541 acggcaactt cagcgaagtt tgcgccatac gggaaggcga aatcaggcgt gatataactg
    18601 ccagttccca ccagtgaacc aaagccggtg ccggtttcgc ctttatgagc aatatccccg
    18661 aagctgactt cgcctttctt gccgcgcacg acgcccggcg ttgccagttg aacatctgcc
    18721 actgcctcac ccaacatttg cgcgccgtgg aacagaattt tctcccgcag attttccgct
    18781 gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg aggcatatgc gcctttatca
    18841 aacaacgcat gatcggtatc accggagata acatgcacgt cctgcggtgg gcagtgcagc
    18901 acttctgctg ccagtttcgt cactacagta tccagaccag taccaatatc cgcaccgcca
    18961 gaatgaacga taaaggtgcc gtccgattcc agtttgatca tgcagttagc ctgatcgata
    19021 tccgggatac ccgatttctg catgataatg gcgacgccgc gaccgatgtg ccagtcacta
    19081 ttttgcggtt tcggtgaaga ccattggatc atctcgcgac cctgacgcag gatctcttcc
    19141 agtgcgcagc tggcggcgga aggaacggag gtcggcgctt taccttcacc gattgcaccg
    19201 agaattttca gctcttgccc ttcgtgtacc cggttacgtt cgataatttc cagttggtcg
    19261 atctgtaact gttcagccag ttccgctaat gccatggtga tagcgaagtt acctttcggt
    19321 gcgccataac cctgataagc accatttggg caaatgttgc tgtagtaggt ggtgacctgg
    19381 aaatcgacgt tatcgcacgg atataacggc agcgacagcg ccggtccgtt acacggtacg
    19441 gtgagtgagt ggttgccgta agggccagtg ttggcgcgga aatccatctt cactgccgtc
    19501 aggcgaccat cttttttcgc tcccagtttg acggtgactt tcgcgacgtg acgagaggtg
    19561 ttagcaataa actcttcttc acgggtgtag cggaacagta ccggacgccc ggtcacgcag
    19621 gttgcccatg cgcacacttc ttccagcagg atgtcctgtt tggagccaaa accgccgcca
    19681 actcgctctt taatgacatg aactttatgc tgtttcatgc ctacgaggcg cgcgacctgg
    19741 cggcgtaagt gccatggtac ctgggtggag gcgtggataa ccagacgatc gccgtccatc
    19801 cgggtaaagc agatatgtgt ttcagtcggg cactgctgtg cctgcgttga gttataggtt
    19861 cgctcaatga tcacatcggc atcggcaaag cctttgtcca tatcgccaat atgaccatga
    19921 atactggcgg cgatattttt gcgagggcga gaaccgatcg ggaagttgat gatcatatgc
    19981 tcgccgcgct gggctgcatg gctgttatcg tcttccagag tatctggcgc accagcaaca
    20041 tacaccaccg gttcatcgtg cacgacaggc gcatcttccg ccattgcttc gtcgatcgac
    20101 attaccggct taagcacttc atattcaacg tcgatgagct tcaatgcttc gagcgcaatt
    20161 tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg
    20221 aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct gaccacccgg ggtatagtag
    20281 atatccgggc aattcaggtg agtaataacg tgaacgacgc ccggtaaggc ttcagctttg
    20341 ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg
    20401 caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg
    20461 gcatcagttt taggataatg cttgccaatg acttcaaggt cgtcacggaa agtcggagcg
    20521 atatcaatgg tggcctgcgg attattttta cgtgcaaccg ccagttcaat gacctgatag
    20581 tattgttgcc agcctgcatc gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg
    20641 cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa tcagagctgc agctggatcg
    20701 ttataaccag actgcaccac gccaacatca accattgcct gttgaaccag acttaactcg
    20761 ttccatttgc ccaaagattc tgcggtacga atatctgcct tctctaactg tgcggcaata
    20821 agcaaggacg cgttaacgat attaccgtta aagattattg cgtcagaacc ggcaaacccg
    20881 aagccatcat cactgttgcg tacagagtgc attcccatgt taaacaacag cttttgcacg
    20941 ttttcgcctg gattaacggt tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc
    21001 atacggcttc ctccccagcc tgttggcagt cggcatacag gtcggctacc agaactcccg
    21061 tgatatagcg tttataggcc acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg
    21121 cgacagcctg ttcaagtgca ttgccttcca gattttgttt ttcgacatca tgcagacgca
    21181 gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa
    21241 cggctgccgt cacgacggtt aaacctgcct gagaacggct aattttgcgg gtcgcacagg
    21301 tgcgatacgg atctttaatg ataatttcgg ttagcaggcg atcgcatggg caggccaggt
    21361 agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag ttcagcatcc agtgccaaca
    21421 ggacgggaag cagcaccgac tcttcctggc gggcggcgat ttcaccacca atggtcgact
    21481 gattacgaac atggcgtgag taaacaaaac cgagggcttc acgcagcgct gcaggaataa
    21541 atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc aatccgcagt gcaccgttat
    21601 cccagtcaac ccagtccagt tccagatcct gtaaggaaat ggcaatcttt ttatcggtac
    21661 gggttggtgt agcattgagt ttgctgcccc cggcgaacca gacggcttca tcctggtagc
    21721 ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct gaaaaattgt tcaatcatct
    21781 tctttcctta tttaaccctc ttccctgcgg gaagagggca tctgtcattt atgccagcgc
    21841 atccatccga cgccacatac tggcagcggc ttttctggct tgtgcataaa tggaatcgca
    21901 atcgaagtta aactgacggt cttcatagac catcacacca ttgaccatca cgctgtgaac
    21961 gctgcctgag cccataccga aagcgatatg cccggcgata ttgtctgcca gcagcggcgt
    22021 cggtgagttg taatcgcaaa tggttaaatc agctttgtaa ccggcttcca gaagtccaaa
    22081 tttcgcgcca aagttgcggc tcatcagttc gttaccgtta gtcagggctt tggcaaaact
    22141 gtcaggccac agcggaccac ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc
    22201 aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg
    22261 atggttgtag ccgacatggt tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc
    22321 gcgctgattg agtagggcga tgtcatcttt cgacaagtac agcccatgag cgaccagcgt
    22381 tttgctatcg atgagatcga attgcgccag tcgtgccagc aggtctttgc cgtaccagtg
    22441 gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga atatgcaaac cacggcctgt
    22501 ggctttcacg gcttcatgta gcatctccag accggcatct ggcacggtaa acggcgcgtg
    22561 agcaccgata tgtgcttcca ccagatacgg ctcggttgct gctttcttcg cctcatcaat
    22621 ctgacgggcg aagcggatgt tttcttctac accttcctgc aactctttga tgccgttgtt
    22681 acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca acttttaaaa atgcgtcgcg
    22741 caatgtggag agcgacccgc cgatatacgc cggagaggca tggtgatcga taaccgatgt
    22801 acatccgctc ttaatcgctt ccagggaaca aatcagcccg ctgtaataga gcgactcttc
    22861 atcaagggcg cgatcgagcc gccaccagag atttttcagc gttgagatga aatccgggca
    22921 gggggcgatg tttgccataa ttccgcggga aagccccgag taaaaatggt tgtgcgagca
    22981 gacaattccc ggcatcacaa tccgaccatg catctctttg tagctggcat cggggtagcg
    23041 ttgcgtcagg gcatcgccga tagcgacaat cacatcattt tcgatagcga tatcaacgcc
    23101 ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat
    23161 tacacctcca cgcggcccag cagataatga tgatgctggt ggacatggct gatgatgcgg
    23221 cacatatcgt tcagctccgg tggtacgttg ttgaactgac cttcgctgtc gatgtttaac
    23281 acccagcttt ggttattcag tcgtactcgt acccggcaat cttccacaag gaagcctggg
    23341 ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg tgattttgtc tttgtatggt
    23401 ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg
    23461 tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc
    23521 gggcagacat ccacacactt gctgcaaacg tagttacatt cgaggcagcg agcggcttcc
    23581 tgggcgacaa acgcgtcacg atcgtcactg ttcaccagag tgatagagat atcgccttta
    23641 cgttgataga tttccgccgg attgacgttg ttccagtatt tatcgttctg gtgggaacgg
    23701 atattttccc gactaaggat ggcatcggtc gcccgacgcg cggttccgac agcagcgaca
    23761 atggaggatg gtccgcgctg tacgtcgccg atcataaaga cgtcagtcag acgagtttcg
    23821 ccattgtggt cgacgtctgg ccagccgttt ttgtccagcg gcacgcccat cgcattcagg
    23881 gcttcagtat cctgctgttc accaatggcg gtgatcaggc tgtctacatg cagtgttact
    23941 gtttcgttgg tttcaaccgg acggcgacgg cctttctcat cgggttcgcc aagcgacata
    24001 acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg gattattcag gaaacggaac
    24061 tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc atgcgggcat ctcttgcagt
    24121 gaacgacggt aaacgatcgt tgctttttct acgcctggaa cgcgtaacgc cgcacgagcg
    24181 cagtccattg cggtgttacc cgccccgaca acgaccacat gtttgcccag cttgagcgct
    24241 gtacccttgt tgtattcacg caggaagggg agtgatttcc aaacattttg gttgtcaccc
    24301 gccagtttca caccgctatt tttatcagtg ccggtggcaa tcagaacata gtggaagccc
    24361 tgatttttta actgttcaac ggtcaaatcg ggtgagcagc catactcaaa tttcacgccg
    24421 tgatcggcaa caaaatcgat atcgtgctga attaactcag ccggaattcg gaactgagga
    24481 atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt caaacagcgt aaccggatgg
    24541 cccgctctgg caaggaagta acctgctgcc agacccgccg gacctgcacc aatcacggca
    24601 accggatggc gtgaaccaga accggctggt ttgtgccagc gttgcttata ttcatcccaa
    24661 cctttttcca gcgcgacttt tttcagttcg cggatattca gcgcactgtc gtaatccagg
    24721 cgggtacagt tgtattggca ctggtgatcg caaatatgac cggtaatggc gggcagagcg
    24781 ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt gttcgccaag cagacggatg
    24841 tattccggaa tatcttgctt gatagcgcag gcagtaacac agggggcaac gtagcaatcg
    24901 gtcagcggca ggtcttctgc cacttcaata cgctcttctg gcttccagtg tttctgggtg
    24961 tattccatgg ttaacgcatc tgctgccagt ctgttcagtc gttcgacgtc aacatggtca
    25021 agtccccagg cgtcggagcc ttccagctcg cgcatgcagg cacttaagcg caaatagccg
    25081 ccaggtttca gcaggtcggt tgccatagta atagggcgaa tacctgtatc aaaaatatcg
    25141 cggatagtca gctgactggc accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg
    25201 cgagagagaa ctgctgcaac attgatggag agcgggaaca gcgcacggcc tgacatatac
    25261 atctcttcac caggcagtgc gcctttattg ttgatggtgc cgagagtgtt agtcagtttt
    25321 acgccaaagc cgagtgattt ttcttttgcc agtgccatca ggcgttccag catttccagt
    25381 gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa
    25441 ccgcagacat cgaggatctc acgaacacgc gcgtatccca gtaaggtcgg gttaagtttc
    25501 acaaaggtgt tgagcccttt ttcttccagc atgtagcggc aaatggcttc gatttcatgc
    25561 ggaggacagc cgtgcatggt ggagagggtg acgccctgca ccatactggt ggggatgcga
    25621 gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg
    25681 tcatcctgga gtaatttatt cagcgtatcg cgatattgcg cgaatttcgg atggtcagat
    25741 gcgtccatca tattgtcgat gaactgttgc attggcggtt gcttaatacc ttcgaggttg
    25801 taaccgacgc tcatattaaa gatgaacgat ttaccggaat cagaaggctg gaacatcgct
    25861 tcgagaaggt gcagggcaaa ccaggctttg aggtattcat cccaggcttt aagcagggta
    25921 aattcggtag accattcggt gttaaagcac tcgtcttcgg catcgataca gggcttttcc
    25981 agctccaggc ggtcaagaat ttggacggtt tttagttcga tgaagcgtcc gccagtcagc
    26041 caggaagtga caatattttg cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga
    26101 gtggcacagg tttcaccgaa gacgctaaca gttttacctt ttacaggtga gtaaaactgt
    26161 tgctcgggaa taccaaagat tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa
    26221 agttcctcaa acggaatggg acgcataata tcccccataa ccctctcctt agcttataca
    26281 gatatcattg agtgaccggt gttgagaaat ccggtggttt agggggatag agcaacaatc
    26341 gcaccactct tgccaggttt ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta
    26401 aaggcttttt gtgaatgaac tcacaggtgc caaatgagaa ttaaaatcac tcctgaaaat
    26461 tattattgtc agcccattta tcactttttc tcttttctca aaatcagtga ttccttatca
    26521 ttatgataaa gagtgctgga gagattaaat atttgaacgc aatcgtttaa ctacattttc
    26581 taaattttag tatacctttt ttgcgctgta ataaagtcgt ccggagtatc aatatctaaa
    26641 attatttctg cattttctat ctcgacggaa taatgagctc ccataaggag tgcctgacgc
    26701 atattggtga cattgggtcg ttggattgcc tgcatcaagc atggttttga cactaaaatc
    26761 ggatggccgg ggatgccatt atggagtggc agtattgcgc catcatttcg taacgaccag
    26821 atttttctaa aaatatcgat ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt
    26881 tctgtttgca ctgcgggtac tgcggccttt actgatgtta gtaaaccctg cgcataatct
    26941 gggttgtgga taatagtgat attgctctgg ttcgcataac gttcgtgcag ttcattacca
    27001 cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt gatacttgta
    27061 tcaagaattg tcccctgttg ccagggtaac atcattttcc attgccccat tcttgatgat
    27121 aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt atttataggg
    27181 gctaataacg cctgataatt gcgttacttc ctgatggatt aagttgggga taatttatcc
    27241 cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta gtcattgatt
    27301 taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg aaaaccagct
    27361 tgcttttttg gctggcagaa ctgctccagg ctagcggcag gcgtgtgtta atcacgacga
    27421 cgacacatat gtttatgcca acgtctcact ggcccgtggt tttctgtcgt gatcccgcca
    27481 tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc tggaaagcga
    27541 accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg caacgaccag
    27601 agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta aaagcgcctg
    27661 atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg atgggaggac
    27721 atactttggg cgcgaaagtg agcacagaaa atgtccatcg ctggtcgcag tttgctgata
    27781 ttaccgggtt aacacctgat gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc
    27841 accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc
    27901 gtttttctca atgtgagaat gcgattgcac aaagcgagct ccttcaaccg ctgcaacaac
    27961 acgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat
    28021 ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg aggttggtat
    28081 gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg cgatggcgca
    28141 aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag tgcgcgccga
    28201 tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga ttgaagagtc
    28261 acttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg ctcgtaacgg
    28321 tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca gcgtccatgg
    28381 tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc aaccgggcga tagcccagag
    28441 tgcggcgcta ttaggatttg atattgccgt tgccgatatt tatcgcgaaa gcctcaatcc
    28501 tgaactattc cctccatcaa ccacgcttct ccatgctgag tcgtttggtg cagcagtgga
    28561 agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc aggatcgtga
    28621 agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg caagtcgccg
    28681 caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtggctgaag aacatattgc
    28741 ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg agatcgccat
    28801 cagcgtgttg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa
    28861 accttctcat ccttccggac accagctggt ggtgattcgc ggtgcgggag atatcgccag
    28921 tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa
    28981 accgacagtg attcgttgta cggtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac
    29041 ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg gaagcgatga aacttaccga
    29101 acgcggattc atccctgtga tggtagatcc cgcctgttca ttgcttgatg aactgaaacc
    29161 gctttgcgtg gtggacgcta ttctggcgaa acagaatttg ggaacgcggg cagatatggc
    29221 accagtaaca atcgcgcttg ggccgggctt tactgcaggg aaggattgtc atgcggtaat
    29281 tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg cgcaggagaa
    29341 taccggtgtt cctggcaata ttatggggca taccacccga cgggtgatcc gtgctcctgc
    29401 tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat
    29461 tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc gtggcttgtt
    29521 gaacgacggc ttggcagtgg ttggtggttt taaaattggt gatatcgatc ctcgtggtga
    29581 aaccgctgat ttctccagcg tttctgataa agcccgggcg attggcggcg gcgtacttga
    29641 ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca aaagaagtgc tggaagtggc
    29701 ttaaatgaaa atggccggat ggtattaccc acccggcctt aaaattactg acaaatcacc
    29761 gttccggttt tgccttcaat cccttctttc gctttgctca atacggtaat cagtgcttca
    29821 cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag catagaacct
    29881 ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag gcgatcgagc
    29941 cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt cagaatgatc
    30001 agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt atcgataacc
    30061 gcgctggcac cacgcagatg gttaccttca cgaataacag gaataccacc accgccaacg
    30121 gtgatcacca cctggcctgc atctaccaga gctttaaccg tttctttttc aataatatca
    30181 accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc tttcagagtg
    30241 taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc gatcggcttg
    30301 gtcgggttga ggaatgccgg atcgttagcg tcgacttcga cctgagtaac cagcgttgct
    30361 accggtttat taatgccccg agaaagcagt tcttctctta aggcgttttg tagatcgtaa
    30421 ccaatataac cctggcttaa ggcaacgcag acagacatcg gcagcattgg cgagtgcgct
    30481 tcagtttttg cagcagcttc aaaggcctgg ttaatcatgc ccacctgcgg cccgttacca
    30541 tgagtgacga taacttcatg tccttgggca attaaatcaa caattgcctg agaagtaatt
    30601 tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg cattcccgcc cagggcgaga
    30661 acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag atccctgaca
    30721 gtggttagag cacgggaggg acaaacggtt tgcgtcgcag gaaacgacct cgcccggctt
    30781 tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg
    30841 cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg agatggcgat
    30901 gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg
    30961 ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcattgcg ctggttaatt
    31021 caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac agtaactgca
    31081 tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct ttagaaattt
    31141 gcaggcgttg agccatcgag aaggtgcagt gatcggtcgc caccacgtca atcgcaccat
    31201 cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt gggctaagaa
    31261 tgaacttcat gccgtcttct gtatcgtaac tgcgttcgtc caacaggaga tattgtgggc
    31321 aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa tccagaccta
    31381 agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt gccaggttaa
    31441 tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggca tgatagcgcg
    31501 gcgcggttaa ccctgcggcg ataaattccg cccgcttgct ggcgatagcc gcatcatttt
    31561 ccgggtgcac ggtggtcagc gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct
    31621 cgtcatcgtt gagtttgtat tgataggtta aatagagttt aaaactgctc agtccttcct
    31681 cgaccatcat cgggatttcg tcgaggattg cgtgattaat gtgctggatc acaccgtgaa
    31741 agctgtaatc gatgaccgct ttatgggcgg cataaccacg ataaacctcc agttgatggc
    31801 gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt gtaccgccac
    31861 acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg ccgacatcaa
    31921 tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca gtggcatcaa
    31981 tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca atcccgcttt
    32041 caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg tttttgatca
    32101 atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata agtgaagagt
    32161 aattactctg ttgccagcca gcttaacggg attgcagcgt acatcgcggc acaggtcacc
    32221 aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt acccgggcca
    32281 aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt tgagaaggtc
    32341 cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac cagtgcttta
    32401 acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt tgggtaaacc
    32461 aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt agctttctgt
    32521 actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc ccaggtcaga
    32581 cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg ggatgtgaag
    32641 aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc cagacgttgg
    32701 gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt gtcaccgcgt
    32761 tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc catacgaccg
    32821 cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa ttccgggcga
    32881 atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc ttcctgcaca
    32941 gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat gattttaccg
    33001 gcataaacca tagatgccat gccgccttcc tggtcggaag taccgcgacc accgatcagc
    33061 tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt gccaatgccg
    33121 acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat ataaccgaga
    33181 acgttgccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat ctcttcttta
    33241 atacgatgta ctacgcgttt ttcgtcgcag ctttcactgg gaatagcaac catgtcgcgc
    33301 aggaagcgag tcatgtccgc ctggtaatct tttgcttttt caagaatcag tttgaatgga
    33361 atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag ggagtagttc
    33421 caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc gcggtagtgc
    33481 ttcacgtcgg tgtcgccttc agtgctgata accagcacca cggcatcttt gttcagcgcc
    33541 agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgctgcgag aacgcccaaa
    33601 ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt tgccgtacgg attacccagc
    33661 acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt ggcgcagtta
    33721 cgtaggattt cccagcccag cgggttaggt tcgccacagg ccaggcccgc catgatggtg
    33781 gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata aatacagtca
    33841 gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctgtagac gtcgaccaga
    33901 taacccagca caccaccggc cattgctccg acaccggctt gcagcagaac gtgcgtcggg
    33961 gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc gtagccttgc
    34021 atgatccagg ttgggatttt ggtgtaacct tcccatgccg tgtcctgtac cacttcccag
    34081 ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt catatccgtg
    34141 acgatgcact cggcaccgag gttcagaatg gcgtcaacgc gttcctgagc agaacctttc
    34201 ggcatgtaaa tcaccgcatt ctgtccgagt tgctgtgctg cccacgccac accgcgcccg
    34261 tggttgccgt cggtggtggt cgcgaaagtc attttttcgc cgatggcatt tttcaggtgc
    34321 tcaaatgaca gcgtttcgat atcaagatga tatttttcac acaataattg agcgatggcg
    34381 tacgcaccgc caagcatttt gaaggcgttc agaccgaatc gttttgattc gtctttgacg
    34441 agaattttct tcacaccaaa aaggtttgcg agatcgtcca gcgcacaaag cggtgttggg
    34501 cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct ttgcgaaaag
    34561 agcgatgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt caatgagaaa
    34621 acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct gatcggcccg
    34681 aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag tgcacctggt
    34741 ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca gctggcttct
    34801 ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcccc ttctttgcag
    34861 gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc atcacgggtc
    34921 agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg tgccagacac
    34981 tgtttttcca gtgcttttaa gccttcgtga tcgttagcgc gcagcaattc ggtacgctgt
    35041 tccatcactt tgtaaggtgc ccatgacttc ggataaacga tgtctgcatc tttgaatgct
    35101 tcttccatgc tggtgacctg acggaagcta ccgccagatg ctttagcatt gtttttcgcg
    35161 acttctacta catccgggat caggtcgtag ccttccggat gggccagggt gacatccata
    35221 ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt gccatagctt
    35281 ggagagtagg cccaggtcat ggcgattttt ttacctttca ggttttccag tgaaccaaag
    35341 tgttcacgta accacgcgag gtcagccatt gactgagtcg ggtggtcaat atcgcattgc
    35401 aggttcacta aagccggacg ctgtggcagt acaccctgct tgtaaccgtc atcaagtgca
    35461 gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc gcgaatacca
    35521 atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc gccgtgagcg
    35581 atttgtgatt tgccttcgtc gagatcttgt tgtgcaaggc cgagcaggtt aagcgcggaa
    35641 gcataagaga agcgggtacg agtggagttg tcgcggaata cggaaatacc taatccacta
    35701 ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc
    35761 agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga
    35821 aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag
    35881 tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg
    35941 gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg
    36001 taaaagacta atttgacgga taatgtgtga ggttgatcac aaattgatgc cagcttcggg
    36061 ttgtttgtaa aatacgaaaa cgattggctt ttcttatcgc gttatttatt tactgctaag
    36121 tatcagaaat aagataaagg gacaggctat ctctcttcag tttgataaac tcaccgccgt
    36181 aattctcata ttgatataga aatgtgatat ctcttccata atttcattag aaagtgataa
    36241 aaatcttatg tttttatcaa agccaatttc gcctgagtta atatcatcat gatctgtgaa
    36301 ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc tatggagctt
    36361 gctactacgc agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt tgccagaatg
    36421 cttgccagcg ttttgcaggt tgagatcgtt gatgaaaact tgtgtcgcgt cgccggaact
    36481 ggcgcgtatg ggaagtttct tggccgccag ttgagcggca actcacgcct gctccgccac
    36541 gtcctggaaa cgaaaactga aaaagttgtg acacagtctc gcttcgatcc cctttgcgaa
    36601 ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc tgggtacgcc tgtcatttta
    36661 caggatcgtt gtgttggggt gataagtttg attgccgtta cccacgagca acaagagcat
    36721 atcagtgata atttacgcga attttctgat tatgttcgcc atatatccac catttttgtt
    36781 tcgaaacttc tggaggatca ggggccagga gataacatca gtaaaatatt cgcgaccatg
    36841 atcgataata tggatcaggg cgtattagtt gttgatgatg aaagtcgggt tcagtttgtt
    36901 aatcagactg ccttaaaaac acttggtgtt gtacaaaata atattattgg gaaacctatc
    36961 cgtttcagac cattaacatt tgagagtaat tttactcatg gacatatgca gcatattgtt
    37021 tcgtgggacg ataaaagtga attaatcatt ggtcaattgc ataacattca gggccgacaa
    37081 ttatttttaa tggcatttca ccaatcgcat accagttttt ctgtagcaaa tgcacctgat
    37141 gaaccacata ttgaacaatt ggttggcgag tgccgtgtta tgcggcaatt aaaacgactc
    37201 attagccgta ttgcacccag cccatccagc gttatggtgg ttggtgaaag cggcacgggt
    37261 aaagaagtcg tcgcccgagc aatccataag ttgagcggaa gacggaataa accctttatt
    37321 gctatcaact gtgccgcgat tccggagcag cttctggaaa gcgaactgtt cggttatgtt
    37381 aaaggcgcat ttactggcgc ttctgccaac ggtaaaacag ggttgattca ggcggcgaat
    37441 acgggcacgc tgtttctcga tgaaataggt gatatgccat taatgttgca ggctaaatta
    37501 ctgcgcgcta ttgaggcgcg tgaaattctg ccgattggtg ccagtagccc aatacaagtc
    37561 gacattcgca tcatttctgc aactaatcag aatttggccc agttcattgc cgaaggtaaa
    37621 ttccgcgaag atctcttcta ccgacttaat gttatcccga taactctgcc accgctgcgt
    37681 gaacgtcagg aagatattga actattggtg cattactttt tacatctgca tacccgtcgt
    37741 ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg aaatattgcg taagcatcgt
    37801 tggcccggaa acctgcgcga gttaagcaat ttgatggaat atctggttaa cgtagttcct
    37861 tcaggtgaag ttatcgacag cacgctattg ccgccaaatc tgctgaataa tggcacaacg
    37921 gagcaaagtg atgtaacaga ggtcagtgag gcgcacctgt cactcgatga tgcgggcggc
    37981 acggcgctgg aggagatgga aaagcaaatg atccgcgagg cgctttcacg tcataacagc
    38041 aagaagcaag ttgctgatga actgggcatc ggcattgcta cgctctatcg caagattaag
    38101 aaatatgagt tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc
    38161 cttttacttc gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca
    38221 gcgacaaaga tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg
    38281 tggtttcgcc agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg
    38341 cactgcgccg gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc
    38401 accttccagc gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc
    38461 tattgctgtg ccgtcgacca gcaccgtaca ggcaccacat tcacccacgc agcacccttg
    38521 tttgacactt agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc
    38581 gtgaagctga aaaggcatcc cgttaatggt gcattcgatg gtaattgttt cgctgtgatt
    38641 cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc
    38701 tggatgagat gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct
    38761 tgcaggacag attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca
    38821 gtctgttcgg catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt
    38881 aattcgctga aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata
    38941 tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt
    39001 ggcggaaaat gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg
    39061 gtgtgaaagc cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc
    39121 gcgtcataaa ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt
    39181 ccaccgtagg tagcgacgtt acggatctgc ggtccagcaa tggacgtggc cgcagcacat
    39241 aacgccggga gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca
    39301 gagccgatac gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta
    39361 tgaatatcaa caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca
    39421 gtgccaccgg cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca
    39481 agggttgctg cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc
    39541 tgccagatgg aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac
    39601 accggtagcc atcttcaccg cgttacgaat agcagcggca acaggaatta ttggtggctc
    39661 acccagtgac ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc
    39721 cagttgtggc agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac
    39781 cacgccgctt ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc
    39841 cattccgccg tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg
    39901 aacgttgagg atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac
    39961 aaaggtacag ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag
    40021 ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa
    40081 cggctcttcc ggtcgttcaa ccagcacgat atggcctttt atcagggtca gattcatcgc
    40141 tgactgatgt agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc
    40201 actgcgcagc gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa
    40261 cggcgtaacg tcggtatctt gagttgaaat aacgcgaacg tcgctgaccg gaacccccac
    40321 ggtttctgcc accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc
    40381 gctttgcacg ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac
    40441 gccgacaggc caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa
    40501 attgccttgc tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc
    40561 tttttcaaga cactccggca accctgcgct gtaaatacgt ttgcccgtga gcggattagc
    40621 atctccttcg cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt
    40681 cgcggcgtca tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg
    40741 catcgcacca gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca
    40801 acgaggataa aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga
    40861 tgcataagcg ccggtgttag acagaacatc cagactataa cctttcaatg ttccgtcgcg
    40921 gttcacgccc atttgcccgt caatggtaaa agcgtggcgg gtacgggttg cgaggaaaca
    40981 ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc
    41041 cgccattggc tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt
    41101 gatgactcgt acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat
    41161 gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag
    41221 cgatgttacg ctttccatgt gacaatgttg aataacgggg gtctgatagt gcccctgtac
    41281 ctggtagtcg gcggcatcga ttgtttgttg gacattaccc gtcgacatcg tgctttgttt
    41341 cagtaaattg ccaccgttat ggattggtgc agcgtcttct gccagcgccg cttctggcgt
    41401 ggtgataacg ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt
    41461 gagttcatcg cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt
    41521 tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc
    41581 agtagcaaat ggaatatcag gcacatcttc ccaggtaaaa atcgccagta cgcccggcaa
    41641 acttctggct tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac
    41701 atatttcgca taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt
    41761 gaccttagca atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc
    41821 ttccatagaa atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc
    41881 aggcaagatt tacgccagaa acgacgataa gcgttagggg aggtaggaag tgtgatgacg
    41941 ttaaaaaaac acagatatga tacgaaacag gactctgaat aggggcagtg atacgtatca
    42001 atatgagtgg cgataaaatc aatgtgataa attgggatta ttacgcatca ggatgggaaa
    42061 tagggtaaaa agtcaggaag ttcgatacct ctacagcgaa tattttgctg atgatacttc
    42121 cctttgccaa atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat
    42181 tgggtatcaa gaggctggct gctatgataa ggcgtcttgt tttttaagcg aggaaagatt
    42241 ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg tgatgttgtt atcggttgga
    42301 ctgcttttgg cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc
    42361 gtttacaccg tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc
    42421 gtaaaagaac tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag
    42481 aaactaaaac tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca
    42541 acgaccaaaa ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca
    42601 gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca
    42661 gcagatggcg gcaataaagg gattgatatc tcagctccac ggggcacacc tatttacgcc
    42721 gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc
    42781 atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta
    42841 aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg
    42901 gcatctgttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt
    42961 tacttgccgc cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca
    43021 gcgcggcact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc
    43081 gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc
    43141 ccgctccaat ttatcttcac ctgatcaatc tgccattttt gctgtatctg attttctata
    43201 ttatcttaat tcaatcgctt cagactattc gcaatcgccg taatacttcc ccattttgtt
    43261 gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aaccctggta aaacacctta
    43321 ttttcaagtt gttaaataag tggttatcta cgcttttaac aaacgattga tggaatattt
    43381 tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg
    43441 ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg
    43501 ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtatccta attatcgtct
    43561 tttgattcac tcagccaaat tacaaaatga aataaagagc cactatacca aagacgaaat
    43621 tcaaggattg ctaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg
    43681 gggaacgttt attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta
    43741 tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat cagatcaggg
    43801 aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgcccga
    43861 ccagattaag cggatgcagg acgtcattag caaccccaaa cgactggatg gagttcccgg
    43921 gcaaatgaat gttgtgcata tccgatttga cagcaactcc cccgtgatgg cagatgtgca
    43981 ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag gtctttatct
    44041 tgatctcctt atacaagcaa tcgacactga tacaattaac gctgaagatt ggcaaaaagg
    44101 tgaccgatta aaatcggttg cgctgctgat tgcttatctg gataaagcca atttctatgt
    44161 tatggaagat tccggagcat gggaagaaga tgcccgatta aacacatctt ccgttgcact
    44221 ggtgacctct ggactggaac gtttgtcgaa cttactctcg aagaaagact ctgtttttgt
    44281 ctctgattta ctacgcgaag ctaaagccaa tgaactggat gaacctcttt caacaacgag
    44341 gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg atttaggggg
    44401 agaatcccca ggttatctgg aaaaagataa acattacaga gaagccgatg cggctttact
    44461 taatgttatt tatcctgcaa atcttgcaaa gataaacacc agaagaaaag agcaggttct
    44521 aaaaatagtt aaaaaactag caggtccgta tggtataaaa agatacgaga aggataatta
    44581 tcagtcggca aatttttggt tcaatgatat taaaaccgat accgatcaaa attcacatgc
    44641 gaaaagagaa aagagcttca ttccatcaac agaagcagaa tggttttttg actcatggta
    44701 tgcaaaatca gcagcaattg tttataaaga atcaagaaag gaagagtatt tgaatgactc
    44761 agtgcaattt atgaatagat cattggccca aataacaggc gagaatatga ttggtgcaaa
    44821 tggcaggagt gtacctgaaa tggcacttcc cgaaagctat aactatatcc acaagtcggg
    44881 aactttgcat gaagcgccaa gccctatcat tccgctaaat tggtcgaaag cttcaatgac
    44941 tctcatgttg aaagagatgt ccaaccttat caatgatgag ggaattaaat agttttcata
    45001 atgagttaca gcacaacgtt gggtattgac tgagcaagat gaacgacaag gacaacgaca
    45061 tcagccttat gaggagcaag aaaatgaaag caaattcgaa aacgatcaga aggatgaaag
    45121 ttaatgttcg gtctgaggaa agtaaatcga aacactcata cgtttgaaac cacttttcaa
    45181 aattggaaag aaaatggcga agatgttgca ttattaatgc ccgaatttag tgctaaatgg
    45241 ttaccgatag cagaagagag tggcagctgg tctggatggg tattgcttgg agaaatattt
    45301 cccttaattt catctgagct cgctgggatg gctttaatgc cagaaacgga aaggttaata
    45361 ggggaatggc tcagtttatc cagttccccg ttaaatctaa agcatcctga actaaaatat
    45421 aatcgtttgc acgtaggtaa agtatttgat ggagtattga accctgctca accactaata
    45481 agaatatgaa caggggaatt gaatctttgg ttagataaag tcacagtctg ccaatacgga
    45541 aacgctccag tgttagacaa aaaatcatta tattggtcca ttcattttgt aattggattt
    45601 agta
//
LOCUS       DAZGCS010000027        44869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGCS010000027 DAZGCS010000000
VERSION     DAZGCS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.027"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(332..874)
                     /gene="ycfP"
                     /locus_tag="ACHK01_001590"
     CDS             complement(332..874)
                     /gene="ycfP"
                     /locus_tag="ACHK01_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO8096558.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPNENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(897..1922)
                     /gene="nagZ"
                     /locus_tag="ACHK01_001591"
     CDS             complement(897..1922)
                     /gene="nagZ"
                     /locus_tag="ACHK01_001591"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO8096559.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFKRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1933..2757)
                     /gene="thiK"
                     /locus_tag="ACHK01_001592"
     CDS             complement(1933..2757)
                     /gene="thiK"
                     /locus_tag="ACHK01_001592"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO8096560.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2738..3379)
                     /gene="lpoB"
                     /locus_tag="ACHK01_001593"
     CDS             complement(2738..3379)
                     /gene="lpoB"
                     /locus_tag="ACHK01_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO8096561.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3393..3770)
                     /locus_tag="ACHK01_001594"
     CDS             complement(3393..3770)
                     /locus_tag="ACHK01_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO8096562.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHK01_001595"
     CDS             complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHK01_001595"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO8096563.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4466..6655
                     /gene="fhuE"
                     /locus_tag="ACHK01_001596"
     CDS             4466..6655
                     /gene="fhuE"
                     /locus_tag="ACHK01_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO8096564.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6715..8148)
                     /gene="ptsG"
                     /locus_tag="ACHK01_001597"
     CDS             complement(6715..8148)
                     /gene="ptsG"
                     /locus_tag="ACHK01_001597"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO8096565.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8443..9240)
                     /locus_tag="ACHK01_001598"
     CDS             complement(8443..9240)
                     /locus_tag="ACHK01_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8096566.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9251..10255)
                     /gene="holB"
                     /locus_tag="ACHK01_001599"
     CDS             complement(9251..10255)
                     /gene="holB"
                     /locus_tag="ACHK01_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO8096567.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10252..10893)
                     /gene="tmk"
                     /locus_tag="ACHK01_001600"
     CDS             complement(10252..10893)
                     /gene="tmk"
                     /locus_tag="ACHK01_001600"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8096568.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10883..11905)
                     /gene="yceG"
                     /locus_tag="ACHK01_001601"
     CDS             complement(10883..11905)
                     /gene="yceG"
                     /locus_tag="ACHK01_001601"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO8096569.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11908..12717)
                     /gene="pabC"
                     /locus_tag="ACHK01_001602"
     CDS             complement(11908..12717)
                     /gene="pabC"
                     /locus_tag="ACHK01_001602"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO8096570.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12837..14078)
                     /gene="fabF"
                     /locus_tag="ACHK01_001603"
     CDS             complement(12837..14078)
                     /gene="fabF"
                     /locus_tag="ACHK01_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO8096571.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14166..14402)
                     /gene="acpP"
                     /locus_tag="ACHK01_001604"
     CDS             complement(14166..14402)
                     /gene="acpP"
                     /locus_tag="ACHK01_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8096572.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14613..15347)
                     /gene="fabG"
                     /locus_tag="ACHK01_001605"
     CDS             complement(14613..15347)
                     /gene="fabG"
                     /locus_tag="ACHK01_001605"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO8096573.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15360..16289)
                     /gene="fabD"
                     /locus_tag="ACHK01_001606"
     CDS             complement(15360..16289)
                     /gene="fabD"
                     /locus_tag="ACHK01_001606"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO8096574.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16305..17258)
                     /gene="fabH"
                     /locus_tag="ACHK01_001607"
     CDS             complement(16305..17258)
                     /gene="fabH"
                     /locus_tag="ACHK01_001607"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO8096575.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17326..18396)
                     /gene="plsX"
                     /locus_tag="ACHK01_001608"
     CDS             complement(17326..18396)
                     /gene="plsX"
                     /locus_tag="ACHK01_001608"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO8096576.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18477..18650)
                     /gene="rpmF"
                     /locus_tag="ACHK01_001609"
     CDS             complement(18477..18650)
                     /gene="rpmF"
                     /locus_tag="ACHK01_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO8096577.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18702..19223)
                     /gene="yceD"
                     /locus_tag="ACHK01_001610"
     CDS             complement(18702..19223)
                     /gene="yceD"
                     /locus_tag="ACHK01_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO8096578.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19422..20006
                     /gene="yceF"
                     /locus_tag="ACHK01_001611"
     CDS             19422..20006
                     /gene="yceF"
                     /locus_tag="ACHK01_001611"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO8096579.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20118..21077)
                     /gene="rluC"
                     /locus_tag="ACHK01_001612"
     CDS             complement(20118..21077)
                     /gene="rluC"
                     /locus_tag="ACHK01_001612"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO8096580.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21650..24835
                     /gene="rne"
                     /locus_tag="ACHK01_001613"
     CDS             21650..24835
                     /gene="rne"
                     /locus_tag="ACHK01_001613"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO8096581.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVVVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25031..25984)
                     /gene="flgL"
                     /locus_tag="ACHK01_001614"
     CDS             complement(25031..25984)
                     /gene="flgL"
                     /locus_tag="ACHK01_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO8096582.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25996..27639)
                     /gene="flgK"
                     /locus_tag="ACHK01_001615"
     CDS             complement(25996..27639)
                     /gene="flgK"
                     /locus_tag="ACHK01_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO8096583.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27705..28646)
                     /gene="flgJ"
                     /locus_tag="ACHK01_001616"
     CDS             complement(27705..28646)
                     /gene="flgJ"
                     /locus_tag="ACHK01_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO8096584.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKVGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28646..29743)
                     /gene="flgI"
                     /locus_tag="ACHK01_001617"
     CDS             complement(28646..29743)
                     /gene="flgI"
                     /locus_tag="ACHK01_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO8096585.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29755..30453)
                     /gene="flgH"
                     /locus_tag="ACHK01_001618"
     CDS             complement(29755..30453)
                     /gene="flgH"
                     /locus_tag="ACHK01_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO8096586.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30506..31288)
                     /gene="flgG"
                     /locus_tag="ACHK01_001619"
     CDS             complement(30506..31288)
                     /gene="flgG"
                     /locus_tag="ACHK01_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO8096587.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31426..32181)
                     /gene="flgF"
                     /locus_tag="ACHK01_001620"
     CDS             complement(31426..32181)
                     /gene="flgF"
                     /locus_tag="ACHK01_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO8096588.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32201..33406)
                     /gene="flgE"
                     /locus_tag="ACHK01_001621"
     CDS             complement(32201..33406)
                     /gene="flgE"
                     /locus_tag="ACHK01_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO8096589.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33431..34126)
                     /gene="flgD"
                     /locus_tag="ACHK01_001622"
     CDS             complement(33431..34126)
                     /gene="flgD"
                     /locus_tag="ACHK01_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO8096590.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     HGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34138..34542)
                     /gene="flgC"
                     /locus_tag="ACHK01_001623"
     CDS             complement(34138..34542)
                     /gene="flgC"
                     /locus_tag="ACHK01_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO8096591.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVGDVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34546..34962)
                     /gene="flgB"
                     /locus_tag="ACHK01_001624"
     CDS             complement(34546..34962)
                     /gene="flgB"
                     /locus_tag="ACHK01_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO8096592.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35117..35776
                     /gene="flgA"
                     /locus_tag="ACHK01_001625"
     CDS             35117..35776
                     /gene="flgA"
                     /locus_tag="ACHK01_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO8096593.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35852..36145
                     /gene="flgM"
                     /locus_tag="ACHK01_001626"
     CDS             35852..36145
                     /gene="flgM"
                     /locus_tag="ACHK01_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO8096594.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36150..36566
                     /gene="flgN"
                     /locus_tag="ACHK01_001627"
     CDS             36150..36566
                     /gene="flgN"
                     /locus_tag="ACHK01_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO8096595.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTIHRGGKKISI"
     gene            complement(36606..38141)
                     /gene="murJ"
                     /locus_tag="ACHK01_001628"
     CDS             complement(36606..38141)
                     /gene="murJ"
                     /locus_tag="ACHK01_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO8096596.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPALLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38251..39174)
                     /locus_tag="ACHK01_001629"
     CDS             complement(38251..39174)
                     /locus_tag="ACHK01_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8096597.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39176..39823)
                     /locus_tag="ACHK01_001630"
     CDS             complement(39176..39823)
                     /locus_tag="ACHK01_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO8096598.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSCAARMYEFSDMAEVESTLEQLANREDGPFVVRLARELGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39834..40418)
                     /gene="rimJ"
                     /locus_tag="ACHK01_001631"
     CDS             complement(39834..40418)
                     /gene="rimJ"
                     /locus_tag="ACHK01_001631"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8096599.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40654..41862
                     /gene="mdtH"
                     /locus_tag="ACHK01_001632"
     CDS             40654..41862
                     /gene="mdtH"
                     /locus_tag="ACHK01_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO8096600.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41926..42573
                     /gene="grxB"
                     /locus_tag="ACHK01_001633"
     CDS             41926..42573
                     /gene="grxB"
                     /locus_tag="ACHK01_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO8096601.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42707..43267
                     /locus_tag="ACHK01_001634"
     CDS             42707..43267
                     /locus_tag="ACHK01_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8096602.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43373..44419
                     /gene="pyrC"
                     /locus_tag="ACHK01_001635"
     CDS             43373..44419
                     /gene="pyrC"
                     /locus_tag="ACHK01_001635"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO8096603.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44493..44738
                     /gene="dinI"
                     /locus_tag="ACHK01_001636"
     CDS             44493..44738
                     /gene="dinI"
                     /locus_tag="ACHK01_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO8096604.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10862 a        12128 c        11169 g        10710 t
ORIGIN      
        1 aggttacagc aatgaaaatg ttcttcgcta ttgagaataa catgaatggt gcattggtca
       61 taccaaaatt gatgttaatc aagttttgtt gctgaaaaga taggcgactt taccgggtga
      121 gatcaaaaaa cagagtttat tgtttattat ttgagcaagc ccggaagggc gatatagggt
      181 gaagcgtata tcgagggcgt ttggtcgtcc tgataagagg cgcatcaggc attgatgctc
      241 gaatgtcgga tgcggcgtga acgccttatc cgacccacag aatccggagc gcatcaggta
      301 ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc
      361 gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct
      421 cgtaataatg atgcagctct tcagatgtcc gctggctgtt aagcgcttca tcattacgcg
      481 acaaaatcac caggcaacga tcgcgattct tctcacggaa gttggtcaca cacttagtgg
      541 caatatcggc atactcttcc gggcgatcaa tcttgccttc catgttctca ttagggaaca
      601 aattagggtt gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt
      661 atccgcccag accaacgccg caaatcagcg gacgctcgtc gacgttcagt tgcaacattt
      721 tgtccacttc tttgagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta
      781 tcaagcgtac atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac
      841 tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag
      901 tgacctgctt tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct
      961 ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt
     1021 gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga
     1081 ttattgcaga ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca
     1141 taactgccca taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca
     1201 aaacccagtt cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc
     1261 ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg
     1321 attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg
     1381 cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg
     1441 gtcgttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct
     1501 ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc
     1561 agcacaggcg caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa
     1621 cccgcctctt gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt
     1681 gctgccggta agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct
     1741 accgccacca ccagatgatt gcgcgaagct gcgcggatct ggcgcaccag ttcacgcaac
     1801 tgggcaggat catgatagtt acgcttaaag agaatcagcc ctcccaccag cggatgcgca
     1861 agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc
     1921 acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat
     1981 aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctgctt tgagcatcag
     2041 cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg
     2101 agtggcatag tcattgacca attgccggtg ctggtcagta ttttccaccc acaccgccgc
     2161 cagttccagc gcgatatcac catctccggc atactcccag tcgatgagtt ttaaccctga
     2221 cgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg
     2281 ccgtggttcc cgcgctttgc gcagacgttt taaccttcgc agccaaccca ctgtccgccg
     2341 cgccggatcg ctttgctgcc agtacagttc cagtaacggc aacagcgtta ttcgccagcc
     2401 aaaacgcggt tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa
     2461 atacgttttt acctcgccgg gcagatagtc gaccaccatc cagtcacgga gatataaatg
     2521 cggcttcggt gcaatacatg cgggtaattg tgataaagcc cgatactggc gcaagaacgc
     2581 gaaccgcggc gcatcaggat cgtgcggctg acgtacaaca aaacgctggc cctggtgttc
     2641 aatgagaaaa ctcccgccac taagcccact atgaaacgtc gtgacgggat gaaactgcgg
     2701 gaaaaagcgc gacagcaatt cgtcgcgcat gatgggatta ttgctgcgaa acggcacctt
     2761 tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag
     2821 cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccgaca ttgcgggcaa
     2881 tgcctatggc tttactacga gtacctaaac tgtcctgcgg cgacaaacct aactgttgct
     2941 tagccatcga cagctgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat
     3001 ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc
     3061 tatcaaccag caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca
     3121 tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttt
     3181 gatcttcgtg ctcaattggg cctggctgct gcgggatcgt cggcaccgag ggcactgtgg
     3241 ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg
     3301 caggttcacg ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt
     3361 agcgactcat ttttgtcatc aagattcacc ccttacaaat atagataaag tctgactttg
     3421 tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatgcta
     3481 acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa
     3541 cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt
     3601 tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat
     3661 gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc
     3721 accagcagta acaacgccag agacaccagc ccaaagcatc cttttctcat cgttacagac
     3781 ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt
     3841 aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa
     3901 taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat
     3961 gctcagctga gacgtcgttc acagtcggaa tgaggatatt cggaatgatc agaatatgcg
     4021 ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat
     4081 cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt
     4141 cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag
     4201 tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag caaaaaatcg
     4261 ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac
     4321 attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttacaa
     4381 acaaaattat tcgcacacta aatataatcg ctcatcaccc tcctccggca tgagcctgac
     4441 gacaacataa accaagggat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata
     4501 tcaagccatc accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc
     4561 tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc
     4621 tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca
     4681 gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga
     4741 tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca
     4801 ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt
     4861 tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat
     4921 ggcactcttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg
     4981 taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg
     5041 cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag
     5101 cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga
     5161 ctcatggctg gaccgctaca acagtgaaaa gaccttcttc tccggcattg tcgatgctga
     5221 tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag
     5281 cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg
     5341 cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat
     5401 gaccctgaag cagcggtttg ctgatacctg gcaagcgacg ctgaatgcca cccactctga
     5461 agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat
     5521 gctggttggg ccatacagta attatggacc tggctttgat tatgtcggcg gcaccggttg
     5581 gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt
     5641 gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgtta
     5701 cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg
     5761 caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata ccacacatat
     5821 gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg
     5881 cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac
     5941 cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta
     6001 tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat
     6061 caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac
     6121 cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat
     6181 ccccggcagc aacggcgaaa ccgcctataa agcagtggat gggacagtca gtaaaggggt
     6241 ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg
     6301 ctatattgca gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt
     6361 taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt
     6421 taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga
     6481 gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc
     6541 gttacagggg aacgtcaata acctgttcga caaaacctac gataccaacg tggaaggttc
     6601 tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac
     6661 acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta cagattagtg
     6721 gttacggatg tactcatcca tctcggtttt caggttgtcg gatttagtac cgaaaatcgc
     6781 ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg
     6841 atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt
     6901 agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc
     6961 tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt
     7021 aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcgggaa
     7081 cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa
     7141 cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat
     7201 cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga tcggctcggt
     7261 gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg
     7321 gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt
     7381 gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat
     7441 gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg
     7501 tacgttccag atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat
     7561 gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga
     7621 accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga
     7681 aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg
     7741 gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccctc cgagtacgcc
     7801 agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc
     7861 aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac
     7921 gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc
     7981 aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa
     8041 attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata ccggcagcat
     8101 cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc
     8161 tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg
     8221 tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat
     8281 ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa
     8341 aacatatgtc acaatatttc aaacgtggtt attcaccgcc caattttcgg cggtgaactt
     8401 tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag
     8461 gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc
     8521 ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg
     8581 ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag
     8641 taatcgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt
     8701 cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct
     8761 gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat
     8821 tgccagcgta tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg
     8881 accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta
     8941 ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg
     9001 taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac
     9061 gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc
     9121 gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc
     9181 cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat
     9241 gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa
     9301 tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtaaca gacattaact
     9361 gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt
     9421 ttgccagttc ggcgaccagg cccggcacat caacattggt cacctgcgca gcaccatgat
     9481 ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt
     9541 catgattaag ggccgctagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg
     9601 cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc
     9661 caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt
     9721 cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac
     9781 aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg
     9841 tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggttagta
     9901 aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcgtgc tcattcagct
     9961 tttcggtgac ctcacgtacc gcatcaatgc ccaacgcatt tttccctttt tcgggagcca
    10021 gggtgtagta atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc
    10081 aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag
    10141 catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct
    10201 gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc
    10261 caactcctta acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg
    10321 ctgggtggca tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc
    10381 gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc
    10441 acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta agtcggggcg
    10501 aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc
    10561 tccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt
    10621 agccagagct ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag
    10681 aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct
    10741 tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat
    10801 acccagttgc tcaagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc
    10861 cagcccctca atgacgatat acttactgcg catttttttc cttaagcact ttcagataat
    10921 cctgcacaga cttgttatgg ctggcaagat tggtattaaa cgtgtgacca cctttaccat
    10981 cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg
    11041 cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg
    11101 tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa
    11161 tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg
    11221 caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca
    11281 ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga
    11341 ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg
    11401 tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa
    11461 gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct
    11521 cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact
    11581 gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa
    11641 agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa
    11701 aaacccgtgg gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc
    11761 cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa
    11821 gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca
    11881 agattatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata
    11941 ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg gcatcactgg
    12001 cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca gagaagcttg
    12061 cacttcgaca agctgataag aggattgtgc cagcaaacgg atacagaatt gtcgcataat
    12121 gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttgccct tccgccagaa
    12181 caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc
    12241 agcgtttgtc tgctcaagat gagagcgaat caatacttgc tcaagacgat tgagatgttt
    12301 aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc
    12361 ctcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc
    12421 cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt
    12481 cagtacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgtggcca
    12541 gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga
    12601 taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg
    12661 cgttgcccga tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc
    12721 ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata
    12781 aaagctaaga aaaaaggccc gcaagcggac cttttataag ggtggaaaat gacaacttag
    12841 atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg
    12901 tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca
    12961 tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag
    13021 tagatagatt ctactgcacc cgccgcacct aacagatgac cggtcataga tttcgtggag
    13081 cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct
    13141 ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca
    13201 atgcctgcat cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc
    13261 ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt
    13321 ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg
    13381 cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca
    13441 ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cacccagcgg cgtactggct
    13501 ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca
    13561 tgaccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc
    13621 aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag
    13681 aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt
    13741 ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt
    13801 atttcaaggc cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag
    13861 gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact
    13921 aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca
    13981 ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac
    14041 atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca gggagggaaa
    14101 aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg
    14161 ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc
    14221 tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca
    14281 acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct
    14341 tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc
    14401 atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg
    14461 ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc
    14521 cttacccccc aataacgcaa atcattttgc actaattatt actcataacc acgcagactg
    14581 cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca
    14641 tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg
    14701 gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc
    14761 tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt
    14821 acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc
    14881 gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg
    14941 atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga
    15001 cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc tttcattcgc
    15061 attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat
    15121 tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc
    15181 agacctttgc cgttagcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc
    15241 gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt
    15301 ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt
    15361 taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg
    15421 tcgacaatgc gtttcgtcag gccagttagc actttgcccg ggccgacttc atagagatgt
    15481 tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac
    15541 tgacgtacca gtgcgtcacg aatggcatca ccattggttt cgcatttcac atcaacgtta
    15601 ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg
    15661 tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg
    15721 cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg
    15781 gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca
    15841 gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc cgccatagcg
    15901 ccagtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac cagacgcacc
    15961 gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga
    16021 ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca
    16081 gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc
    16141 tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct
    16201 tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt
    16261 ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc
    16321 gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta
    16381 atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta
    16441 ccgtggcgat ccagcgtcac cacgacattg tccatagaca taccgagttt tttcgccgtt
    16501 gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca
    16561 agattatttg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg
    16621 aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac acggtcagca
    16681 ttaggcagcg tcagcaactc accatagcta ccgtcggcat gcagatgggt ggagatgatt
    16741 cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata
    16801 gtcccacgat cggttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca
    16861 tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct
    16921 gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccagcat gctttgaatc
    16981 tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca
    17041 atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg
    17101 gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca
    17161 atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg
    17221 ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt
    17281 tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc
    17341 gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc
    17401 aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa
    17461 agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca
    17521 ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag
    17581 ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag
    17641 cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa
    17701 gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc
    17761 gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcgag
    17821 accctttact tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac
    17881 cacctcttca gccaggactg agcccataat ggcaaattgc accaacattg tgctgtcaca
    17941 atcgacgttg gcccctaaat cgagaaccac cgttttgccc ttttgctgat gcggcaatac
    18001 cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc
    18061 catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccaa
    18121 ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg aaggccggac
    18181 atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc
    18241 agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg
    18301 cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc
    18361 tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt
    18421 ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac
    18481 ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt
    18541 ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa
    18601 cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact
    18661 cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg
    18721 gctaatacgg caaatgggtt tggcttttgc gcttcttcag gcagttcacc aaagaccatg
    18781 tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg
    18841 atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc
    18901 ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa
    18961 tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc
    19021 gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg
    19081 gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca
    19141 ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc
    19201 aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca
    19261 aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg
    19321 ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta
    19381 aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag
    19441 cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg
    19501 cagcaccaga ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc
    19561 gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg
    19621 gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa
    19681 atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg
    19741 cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt
    19801 tccgtcacct gagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact
    19861 gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac
    19921 gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg
    19981 aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg
    20041 caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc
    20101 aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct
    20161 tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac
    20221 gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca
    20281 aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc
    20341 acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca
    20401 tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg
    20461 atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc
    20521 gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat
    20581 tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg
    20641 tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa
    20701 ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta
    20761 ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt
    20821 tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc
    20881 acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact
    20941 tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc
    21001 aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat
    21061 ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt
    21121 tttctatgct tatatttagt tttacacctt attacttcac tacgtgatca ctttattgat
    21181 ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg
    21241 gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc
    21301 atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg
    21361 cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc
    21421 ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc
    21481 gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata
    21541 accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt
    21601 actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat
    21661 gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct
    21721 gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg
    21781 taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg
    21841 tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc
    21901 tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat
    21961 cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg
    22021 tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc gccgtatcga
    22081 aggcgacgac cgtaccgaat taaaagaagc actggcgagc cttgaactgc cggaaggcat
    22141 ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc aatgggattt
    22201 aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc
    22261 gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca
    22321 ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac gtcagcatat
    22381 cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc
    22441 gctgttcagc cactaccaga tcgagtcgca gatcgaatcc gccttccagc gtgaagtccg
    22501 tctgccgtcc ggtggttcca ttgttatcga cagcaccgaa gcgttaaccg ccatcgacat
    22561 caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct
    22621 cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt
    22681 tatcgacttc atcgacatga cgcctgtacg ccaccagcgt gcggtagaaa accgtctgcg
    22741 tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct
    22801 gctggaaatg tcccgtcagc gcctgagccc atcactgggt gaatccagtc atcacgtttg
    22861 tccgcgttgt tctggtactg gcaccgtgcg tgacaacgaa tcgctgtcgc tctctattct
    22921 gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc
    22981 tgtgccaatc gcttcttacc tgctgaatga aaaacgttct gcggtaaatg ccattgaaac
    23041 tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa ccccgcacta
    23101 ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca tgctgccgaa
    23161 gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc
    23221 ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcgc caacgccagc
    23281 tgaacctgcc gcgcctgttg tagccccagc accgaaagct gcaccggcaa caccagcaac
    23341 tcctgcacaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg
    23401 tagtgaagaa accaaaccga ccgagcaacc agcaccgaaa gcagaagcga aaccggaacg
    23461 tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga
    23521 cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg
    23581 ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaagctg aggtaacgga
    23641 aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa
    23701 cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt
    23761 tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca
    23821 gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt
    23881 ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga
    23941 actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa
    24001 caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca
    24061 cctgcgcgta agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc
    24121 gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat
    24181 ccgctatcca attgtacgtc cgcaagatgt acaggttgaa gagcagcgcg aacaggaaga
    24241 agtacatgtg cagccgatgg tgactgaggt ccctgtcgcc gccgctatcg aaccggttgt
    24301 tagcgcgcca gttgttgaag aagtggccgg tgtcgtagaa gcccccgttc aggttgccga
    24361 accgcaaccg gaagtggttg aaacgacgca tcctgaagtg atcgctgccg cggtaactga
    24421 acagccgcag gtgattaccg agtctgatgt tgtcgtagcc caggaagttg cagaacaagc
    24481 agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg tcgaaactgc
    24541 tgaggttgta gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tagtcgctga
    24601 agtcgcagca gaagttgaaa cggtagctgc ggtcgaacct gaggtcaccg ttgagcataa
    24661 ccacgctacc gcgccaatga cgcgcgctcc agcaccggaa tatgttccgg aggcaccgcg
    24721 tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca
    24781 tacggcaaca catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    24841 agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatc tttgaatcaa
    24901 catattaaaa ttcatcgaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa
    24961 tgcccgcccc agcgatccca gagcaggcag acaaaacata cccagtttca tgatatgttt
    25021 taaagcgaaa ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt
    25081 tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt
    25141 ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaatg
    25201 aatccagcga ctccagttcg ttcagctgcg tgcctaattc cgcgcgaaca gtcagcacat
    25261 tgttcagcga gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt
    25321 tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcacta tccagcatgg
    25381 caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgttg ctggtaatac
    25441 tgtcgaaaat tttgtcaccc gtgtgcccta tcaccatcga acgcgaagca tcgacctgtt
    25501 gtttaatact ttctgctcca ccgacgtatt tccctttctc ttcgctaaac ggcgcagtct
    25561 ctgttttata accggcaaaa atgtagcgcc cgttaccgtc agtggtgttc gccagattca
    25621 gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca
    25681 aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct
    25741 ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt
    25801 actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat
    25861 cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt
    25921 ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac
    25981 gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg
    26041 cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc
    26101 atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc
    26161 ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga
    26221 ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc
    26281 cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc
    26341 gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt
    26401 cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa
    26461 tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt
    26521 gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc
    26581 atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc
    26641 gggcttaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt
    26701 gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt
    26761 ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa
    26821 tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga
    26881 agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga
    26941 gtaaccattg gccatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc
    27001 aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga
    27061 cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt
    27121 agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg
    27181 gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat
    27241 cagcgcctgg cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa
    27301 gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt
    27361 cgacatctgc tcatagcggg cagtcaggcc gctactttgc gtctgcgcag cacgtaactg
    27421 gttggtaata aacgcatcat actcacgctg cacaccagaa acgtagacgc cattgccaac
    27481 ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata
    27541 tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc
    27601 gttcagtccg ctcatggcgt tattaatcaa gctggacatg gaggttcctt ataagccttc
    27661 aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca
    27721 atgttcatac tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctgaatcatg
    27781 ttggtgagtt tgcgggcata gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc
    27841 gccccctgtt ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgcgttaac
    27901 agcccaacgt aatccgacaa ggcttccaga tacgagctgt agacgcgaaa cttcgctttt
    27961 actttcttcg cttcgccgtt ttcatattca gtcgtggtga tttcagtaac tggccctttc
    28021 cagttgccag aggctttgac accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg
    28081 atttgccgtt gcccccaacc agattccagt gccgcctgag cgaggatcaa atgatgtggc
    28141 acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta
    28201 ctgtcacccg gcagcgaatc atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa
    28261 agcgcctgat tttgataacg caccacagtt tcgagcggga atttcatcgg tgctgctggc
    28321 gtggactcct ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc
    28381 cccagacctt tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac
    28441 agacgagtgt gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc
    28501 aacatcatct gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc
    28561 ggatcttcgc ccactttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt
    28621 gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg
    28681 cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga
    28741 gcgcgcgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc
    28801 tctggcgtaa atcgatctgc gtttgtggag taaccacagt ctgtccacca ccaaacggtg
    28861 tatctggctg gctgacattg gcctgacgat tgactgttac tgagagattt ccctgcgcta
    28921 ccgcgcagct gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa
    28981 tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata tctgcaagga
    29041 aacggacctg ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg
    29101 cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc
    29161 tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca
    29221 attcacgttc aataaccgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa
    29281 cactgctacc gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg
    29341 catacacctg actgtcaacg cccttaagcg gcgtcatcaa caacgtacct ccacgcaagc
    29401 ttttggcatt tcccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg
    29461 gaggaagtga cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg
    29521 tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg
    29581 tctgggttgt ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg
    29641 agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg
    29701 tcgtgaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat
    29761 tggcgacagg ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta
    29821 gccattgcct acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct
    29881 gccgctgata gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt
    29941 aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac
    30001 cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa
    30061 cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata
    30121 gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga
    30181 gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg
    30241 tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa
    30301 aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg
    30361 caccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga
    30421 aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac
    30481 cggtgaacct gccaccggtt aagccttaga gttgcgtcag tttttgcagc atctgatcgg
    30541 tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca
    30601 gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc
    30661 cgttcaggcc cggcgtgctt tcgttcggtg caccagagga ttgcgtttcg gtgtagaggt
    30721 tttcgccaat gctctccagc ccggtgtcat tcataaaggt ggtgagattg agctgcccaa
    30781 cctgaaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg
    30841 tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg
    30901 tcaccagctg cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgaac
    30961 catctggcaa catcacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt
    31021 tggtctgcga caggtttccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg
    31081 tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc
    31141 gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac
    31201 tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg
    31261 ttttggcgat ccataatgaa ctgatcataa aatgtcctgt agtggattag tgacaagcag
    31321 agcagaagca cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga
    31381 gggaatagtc cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag
    31441 cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc
    31501 aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc
    31561 ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtatcg gcccacgcgt
    31621 ggcctggctt tctgcgctta aacgaaaaat gccgtcgtca ccgcgttgca cttcgctgcc
    31681 cgtggctttc accagtttca gacgccccac tggcgcaacc gtatttgccg gatcgcccgg
    31741 attgagcgcc gagattgtgc catcggcagc aatagtgatt tccgcccctt ccggcacagc
    31801 aattggccca gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc
    31861 aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggact gcacggccag
    31921 ccagccatcc tgctgcaatg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg
    31981 cgtcatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc
    32041 ttccaccggc actgcacgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt
    32101 ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg ctcccatcgc
    32161 ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg
    32221 tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt agactgatag ttacgctggg
    32281 caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc aacgcaccgt
    32341 tggtcagggt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt
    32401 gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg
    32461 ccagtacaat ctgccccagc agttgtgttt gttcgttgga atagttgccg acaaccgtac
    32521 cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg
    32581 ccacaatatt gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg
    32641 tggcgggatc tgcgccgtta attgcgccag tggtaatatt ctctgttgga tttgaggtca
    32701 gaacgccatt ggcattaaac accagcttca ttgcaggctc ggctgtacct gttggatcac
    32761 tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaag tagacgctca
    32821 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
    32881 ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga
    32941 tctgcatcga tgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat
    33001 tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
    33061 gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac
    33121 ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag
    33181 caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac
    33241 cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg
    33301 tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt
    33361 tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa
    33421 atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga
    33481 gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg
    33541 gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt
    33601 tcggcgcagt gctgccgtca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg
    33661 tcagttcacc aatatcaatg gtgcgcacaa ccgcaccatt tttatcggtg atggtggccg
    33721 taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagctc
    33781 cttcttcact gccggttccc gcaagaacag tggtgccgtg gatcatcacg ccgtgaccga
    33841 tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagtg
    33901 tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct
    33961 cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca
    34021 gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac
    34081 tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta
    34141 ttgaccgagc gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacgtt
    34201 ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tccccgacga catcaacgtt
    34261 cggcattttt acgtagccct ttgcatctgc cagcggatta cccggttcat aaaccagttt
    34321 gtccggggcc tgactttcta taacatcgcc aacctttacg ccgcctgtcg cagcacctgg
    34381 tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt
    34441 cacgctatca gcatttgcca gattactggc cgccacgttc aggcgctggg actgggcagt
    34501 taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg
    34561 taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg
    34621 caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag
    34681 cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc
    34741 cgggatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg
    34801 ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt
    34861 atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc
    34921 ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc
    34981 aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa
    35041 atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa
    35101 atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt
    35161 cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt ttagcgccca
    35221 actcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc
    35281 gccatgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa
    35341 tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg
    35401 aaattatgtg gttgccgcga tgcccattgt gcggggagga aagctggaag ctggcaatgt
    35461 caaactaaaa cgcggacggc tggataccct gccaccgcgt acggtgctgg atatcaatca
    35521 acttgttgat gccattagcc tgcgcgatct atcacccgat caacctatcc agttaaccca
    35581 gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga
    35641 tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc
    35701 gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag atgggaatat
    35761 tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca
    35821 tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa
    35881 gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acagccgggc
    35941 ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact
    36001 gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat
    36061 tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc
    36121 gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga
    36181 tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta
    36241 tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg
    36301 cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca aatagcgttg
    36361 atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc
    36421 aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt
    36481 tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaatccatc
    36541 gcggcggtaa aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg
    36601 cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca
    36661 gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc
    36721 atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc
    36781 accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg cggggtaaag
    36841 attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca
    36901 atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt catcaattgc
    36961 gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa
    37021 aagccaggag ccaaaacttt cactacaatc aggccaatca aacccaccga gtaggcaatt
    37081 aacgcccgct gggtcatcag cgcatcaaag gcggtaaatt taccgtactg gaacagcgaa
    37141 acggtcaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa
    37201 caaagacgca gcccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa
    37261 ctcttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accagacgga
    37321 aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag
    37381 gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggt
    37441 cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc
    37501 agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc
    37561 gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg
    37621 aaaccaatca tgctgatgtt aagcagtgct ggagcaaacg ccggaatcga gaagcggttc
    37681 cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag
    37741 gtaatcttta gtagctggct ggtcagggca aatttgtcgg cagtgtcagc gaagcctggc
    37801 gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc
    37861 gcgagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc
    37921 ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag
    37981 atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct
    38041 gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg
    38101 gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc
    38161 cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg
    38221 gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt
    38281 gtcaacgata cgttgcgcca gcacggcctg ttcgccggcg gtttgcggaa ctgtctgatt
    38341 ttgcacacat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgtt caagagtgct
    38401 ctgccaacca ggaatcggtt tatgcactac gccctgcccg cgctcctcac gccattcgcg
    38461 catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact
    38521 tccggcacgg cgatgcatac aggtggtgat ctgcaaagga ccggccgaaa aatggtgctc
    38581 ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt
    38641 gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa
    38701 ataaagatcg tgtggcccga cactattgct acgatgttta tccattctta gcgaagctgc
    38761 ggtagcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac
    38821 catcaacgtc agttttttac gcgccgccag ttccaccagc cgttcagcat cgcgcagatt
    38881 ttctgccagc ggtttatcga cacagacatg tacacccgca ttgagtaacg tactgaccac
    38941 gtcaaagtgg ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg cggcaaggct
    39001 ggataacgaa tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg
    39061 cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca
    39121 cgctttttgc gcaatgccac ctaatcccac tacgccgata cgtaattttt tcactttaat
    39181 ctcccagatg ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca
    39241 gggcttcgac gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc gtcaccgccg
    39301 gctgatcttc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccagtt
    39361 cgcgggccag acgcaccaca aaaggaccat cttcgcgatt tgccagttgt tccagcgtcg
    39421 actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcagctg cgcagttcac
    39481 ctggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttcc gctgcgctca
    39541 gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac
    39601 caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca
    39661 cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga
    39721 ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc
    39781 aaccaatcac gcgagcttca agggcagtaa gttgatattt catgagattc tccttagcgg
    39841 ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca
    39901 atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca
    39961 ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc
    40021 tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt
    40081 tgcccaatcg aataaccgag atagcaggca tgaaaagagc cacgaacaac attggaaaaa
    40141 ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa
    40201 ccttgtttat gaaattcgtt aatcatcccc agcctggcct gccagcctga tggataacag
    40261 tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa
    40321 tccgcaagac gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt
    40381 aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta
    40441 acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc
    40501 aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt
    40561 ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag
    40621 aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac
    40681 ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc
    40741 ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt
    40801 attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt
    40861 gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga
    40921 ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg
    40981 ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt
    41041 ccacaacaac gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc
    41101 attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca
    41161 ggggccgttt tgtttgtgct gtgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa
    41221 ctctccaccg tacacacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt
    41281 tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg
    41341 atgctgccaa ttatggtcaa cgacgtggct ggcgcaccct ctgccgttaa atggatgtat
    41401 gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag
    41461 cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg
    41521 ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc
    41581 gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga
    41641 gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt
    41701 tatatcggtg gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg
    41761 tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt
    41821 cagaaacgcg ccgcgcgtcg tttgcttgaa cgcgacgcct gatttgatac tcccccctga
    41881 ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt
    41941 tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc
    42001 gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa
    42061 aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag catggacatc
    42121 gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc
    42181 attgaagagt ggctgcgcaa ggtcaatggc tacgcaaaca aactgctgtt gccgcgtttt
    42241 gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag
    42301 aaagaggcca gcgcgggtaa tttagccgac ctgctggccc actctgacgg tctgattaag
    42361 aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat
    42421 ggcgaacttt cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta
    42481 gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca
    42541 caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt
    42601 cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc
    42661 tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga acaagttttt
    42721 atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata
    42781 caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga
    42841 tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacttga ccagccaaat
    42901 tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc
    42961 cctgttcggt agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga
    43021 taaagaaaaa ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc
    43081 ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa
    43141 ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat
    43201 ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac
    43261 ggattaatga cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg
    43321 cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc
    43381 accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg
    43441 cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctattgtaat
    43501 gcccaatctg gctccgcccg tgaccactgt tgaggctgcc gtggcgtatc gccagcgtat
    43561 tcttgatgcc gtacctgccg ggcacaattt caccccattg atgacctgtt atttaacaga
    43621 ttcgctggat cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca ccgctgcaaa
    43681 actttatccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat
    43741 catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt
    43801 gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga
    43861 acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga
    43921 tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca
    43981 tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt
    44041 acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt
    44101 taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag
    44161 ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt
    44221 tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt
    44281 ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc
    44341 tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgggg aaacggtacg
    44401 ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta
    44461 taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa
    44521 cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc
    44581 agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt
    44641 tatcggttat tggcgcaaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa
    44701 cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg
    44761 ccgggtcgcc ccggcttttt tattttactt acaaattttg agtacaaaat ttctacactt
    44821 cctcaaaata attgcacaat atgctaaaaa aatatgaatg acatgctgt
//
LOCUS       DAZGCS010000028        44868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGCS010000028 DAZGCS010000000
VERSION     DAZGCS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.028"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            306..1076
                     /gene="yjjP"
                     /locus_tag="ACHK01_001637"
     CDS             306..1076
                     /gene="yjjP"
                     /locus_tag="ACHK01_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO8096605.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1067..1540
                     /gene="yjjB"
                     /locus_tag="ACHK01_001638"
     CDS             1067..1540
                     /gene="yjjB"
                     /locus_tag="ACHK01_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO8096606.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1647..2186
                     /gene="dnaT"
                     /locus_tag="ACHK01_001639"
     CDS             1647..2186
                     /gene="dnaT"
                     /locus_tag="ACHK01_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO8096607.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2189..2926
                     /gene="dnaC"
                     /locus_tag="ACHK01_001640"
     CDS             2189..2926
                     /gene="dnaC"
                     /locus_tag="ACHK01_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO8096608.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2975..3469
                     /gene="yjjA"
                     /locus_tag="ACHK01_001641"
     CDS             2975..3469
                     /gene="yjjA"
                     /locus_tag="ACHK01_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO8096609.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3723..6014
                     /gene="opgB"
                     /locus_tag="ACHK01_001642"
     CDS             3723..6014
                     /gene="opgB"
                     /locus_tag="ACHK01_001642"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO8096610.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHK01_001643"
     CDS             complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHK01_001643"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO8096611.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYQFATLAETYERDVINNRELI
                     KGVITF"
     gene            7315..8229
                     /gene="lgoR"
                     /locus_tag="ACHK01_001644"
     CDS             7315..8229
                     /gene="lgoR"
                     /locus_tag="ACHK01_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO8096612.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8444..9805
                     /gene="lgoT"
                     /locus_tag="ACHK01_001645"
     CDS             8444..9805
                     /gene="lgoT"
                     /locus_tag="ACHK01_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096613.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9854..11518)
                     /gene="tsr"
                     /locus_tag="ACHK01_001646"
     CDS             complement(9854..11518)
                     /gene="tsr"
                     /locus_tag="ACHK01_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO8096614.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11637..12083)
                     /gene="hpaR"
                     /locus_tag="ACHK01_001647"
     CDS             complement(11637..12083)
                     /gene="hpaR"
                     /locus_tag="ACHK01_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO8096615.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12355..13644
                     /gene="hpaG"
                     /locus_tag="ACHK01_001648"
     CDS             12355..13644
                     /gene="hpaG"
                     /locus_tag="ACHK01_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO8096616.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13641..15107
                     /gene="hpaE"
                     /locus_tag="ACHK01_001649"
     CDS             13641..15107
                     /gene="hpaE"
                     /locus_tag="ACHK01_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8096617.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15109..15960
                     /gene="hpaD"
                     /locus_tag="ACHK01_001650"
     CDS             15109..15960
                     /gene="hpaD"
                     /locus_tag="ACHK01_001650"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO8096618.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15970..16350
                     /locus_tag="ACHK01_001651"
     CDS             15970..16350
                     /locus_tag="ACHK01_001651"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO8096619.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16461..17264
                     /gene="hpaH"
                     /locus_tag="ACHK01_001652"
     CDS             16461..17264
                     /gene="hpaH"
                     /locus_tag="ACHK01_001652"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO8096620.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17275..18063
                     /gene="hpaI"
                     /locus_tag="ACHK01_001653"
     CDS             17275..18063
                     /gene="hpaI"
                     /locus_tag="ACHK01_001653"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO8096621.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            18238..19614
                     /gene="hpaX"
                     /locus_tag="ACHK01_001654"
     CDS             18238..19614
                     /gene="hpaX"
                     /locus_tag="ACHK01_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO8096622.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19624..20514
                     /gene="hpaA"
                     /locus_tag="ACHK01_001655"
     CDS             19624..20514
                     /gene="hpaA"
                     /locus_tag="ACHK01_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO8096623.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20765..22327
                     /gene="hpaB"
                     /locus_tag="ACHK01_001656"
     CDS             20765..22327
                     /gene="hpaB"
                     /locus_tag="ACHK01_001656"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO8096624.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22345..22857
                     /locus_tag="ACHK01_001657"
     CDS             22345..22857
                     /locus_tag="ACHK01_001657"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO8096625.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23250..25400
                     /gene="btsT"
                     /locus_tag="ACHK01_001658"
     CDS             23250..25400
                     /gene="btsT"
                     /locus_tag="ACHK01_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO8096626.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25450..25653
                     /gene="yjiX"
                     /locus_tag="ACHK01_001659"
     CDS             25450..25653
                     /gene="yjiX"
                     /locus_tag="ACHK01_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8096627.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25664..26620
                     /gene="yjiA"
                     /locus_tag="ACHK01_001660"
     CDS             25664..26620
                     /gene="yjiA"
                     /locus_tag="ACHK01_001660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8096628.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(26698..29238)
                     /locus_tag="ACHK01_001661"
     CDS             complement(26698..29238)
                     /locus_tag="ACHK01_001661"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO8096629.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(29590..29754)
                     /gene="yjiS"
                     /locus_tag="ACHK01_001662"
     CDS             complement(29590..29754)
                     /gene="yjiS"
                     /locus_tag="ACHK01_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO8096630.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            29931..31343
                     /gene="yjiR"
                     /locus_tag="ACHK01_001663"
     CDS             29931..31343
                     /gene="yjiR"
                     /locus_tag="ACHK01_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8096631.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(31585..32529)
                     /locus_tag="ACHK01_001664"
     CDS             complement(31585..32529)
                     /locus_tag="ACHK01_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8096632.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            32996..34228
                     /gene="mdtM"
                     /locus_tag="ACHK01_001665"
     CDS             32996..34228
                     /gene="mdtM"
                     /locus_tag="ACHK01_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO8096633.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVGDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTESRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            34269..35549
                     /gene="yjiN"
                     /locus_tag="ACHK01_001666"
     CDS             34269..35549
                     /gene="yjiN"
                     /locus_tag="ACHK01_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO8096634.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            35665..36816
                     /gene="yjiM"
                     /locus_tag="ACHK01_001667"
     CDS             35665..36816
                     /gene="yjiM"
                     /locus_tag="ACHK01_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO8096635.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            36826..37593
                     /gene="yjiL"
                     /locus_tag="ACHK01_001668"
     CDS             36826..37593
                     /gene="yjiL"
                     /locus_tag="ACHK01_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO8096636.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            37590..37847
                     /gene="ytiA"
                     /locus_tag="ACHK01_001669"
     CDS             37590..37847
                     /gene="ytiA"
                     /locus_tag="ACHK01_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO8096637.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            37912..38772
                     /gene="yjiK"
                     /locus_tag="ACHK01_001670"
     CDS             37912..38772
                     /gene="yjiK"
                     /locus_tag="ACHK01_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO8096638.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            38840..40018
                     /gene="yjiJ"
                     /locus_tag="ACHK01_001671"
     CDS             38840..40018
                     /gene="yjiJ"
                     /locus_tag="ACHK01_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096639.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(40031..40585)
                     /gene="kptA"
                     /locus_tag="ACHK01_001672"
     CDS             complement(40031..40585)
                     /gene="kptA"
                     /locus_tag="ACHK01_001672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO8096640.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            40835..41518
                     /gene="yjiH"
                     /locus_tag="ACHK01_001673"
     CDS             40835..41518
                     /gene="yjiH"
                     /locus_tag="ACHK01_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO8096641.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEMTERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            41515..41976
                     /gene="yjiG"
                     /locus_tag="ACHK01_001674"
     CDS             41515..41976
                     /gene="yjiG"
                     /locus_tag="ACHK01_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO8096642.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLIGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            41989..43161
                     /gene="iadA"
                     /locus_tag="ACHK01_001675"
     CDS             41989..43161
                     /gene="iadA"
                     /locus_tag="ACHK01_001675"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8096643.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            43226..44137
                     /gene="hypT"
                     /locus_tag="ACHK01_001676"
                     /gene_synonym="qseD"
     CDS             43226..44137
                     /gene="hypT"
                     /locus_tag="ACHK01_001676"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO8096644.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(44130..44522)
                     /gene="iraD"
                     /locus_tag="ACHK01_001677"
     CDS             complement(44130..44522)
                     /gene="iraD"
                     /locus_tag="ACHK01_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO8096645.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(44519..44602)
                     /gene="idlP"
                     /locus_tag="ACHK01_001678"
     CDS             complement(44519..44602)
                     /gene="idlP"
                     /locus_tag="ACHK01_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO8096646.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(44718..44861)
                     /gene="ytiD"
                     /locus_tag="ACHK01_001679"
     CDS             complement(44718..44861)
                     /gene="ytiD"
                     /locus_tag="ACHK01_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO8096647.1"
                     /translation="MADYAEINNFPPELSSGGDKYFHLHNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT        10489 a        11837 c        11507 g        11035 t
ORIGIN      
        1 cacaaccaat atttattcaa tgaaatctga tggttttaac ttgttaacat taaaaaacgt
       61 gcccggttat ctattatcct gattcgtaaa ggccaaacaa taaattcatt atgatgataa
      121 tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac cgagcttctc
      181 ccgataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa caaccgtgct
      241 tcatgataca aatggattct cattatctga acaatacgca gcacgtctat gacaaagggc
      301 gagttatgca aactgagcaa cagcgagccg taacacggct ttgtatccag tgtggattat
      361 ttcttctgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca cgactgggtc
      421 gggcgctggg aatggacagc gttgaaagtt ctatctcttc gaacgccata gtgctgacaa
      481 ccattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc ggcattaata
      541 tgcatgtggt gactgaagtc cagcacattg tgattctggc ggagcaccat ctgctggatt
      601 acaaaggcgt agagaaacga tttagccaaa ttcagccatt acgttaccca agatggctgg
      661 tagccttaat ggttggcctt tcctgcgcct gtttctgtaa actcaataaa ggtggctggg
      721 atggtgccgt catcaccttc tttgccagta cggccgcgat gtatatccgc cagctactgg
      781 cacaacgtca tcttcatcca cagatcaact tttgcctcac cgctttcgcc gccaccacta
      841 tttccggttt gcttttgcaa cttcccacct tcagcaatac acccaccatt gcgatggccg
      901 ccagcgttct gctgctggtg ccgggatttc cgttgataaa tgccgtcgcc gatatgttta
      961 aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg acactggcta
     1021 cctgcgtcgg cgtggtgatg gcactgacga tttgggggct acgcggatgg gtgtgatcga
     1081 gtttctgttt gcattggcgc aggatatgat cctcgccgcc attccagcgg tcggctttgc
     1141 gatggtgttc aacgttcccg ttcgggcatt acgctggtgt gcgctgcttg gcgcgattgg
     1201 tcatggttca cgaatgatct taatgaccag cgggctgaat attgagtggt caacctttat
     1261 ggcttctatg ctggtcggca ccattggtat tcaatggtcg cgctggtatc tggcgcatcc
     1321 gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg cttataccgc
     1381 aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga tgattaccct
     1441 gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg gtctttccat
     1501 tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg cttaagcaca
     1561 cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct cccttctcgt
     1621 tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg gacgtcgttg
     1681 gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa ggcggtgtgg
     1741 ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca cgcctggctg
     1801 aactgctggc gctggaagaa aagctggcgc gtccgggaag cgatgtcgct ctggacgatc
     1861 aactctatca ggaaccgcaa gccgctcccg ttgctgtccc catggggaaa ttcgccatgt
     1921 atccggactg gcaacccgat gccgacttta tccgcctggc ggcgctatgg ggcgtggcgc
     1981 taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg caggcggaag
     2041 gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg caaatcggtc
     2101 gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagcgaa cctgacagcc
     2161 aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga tgcaacgcct
     2221 gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag aactactggc
     2281 ctggcagaaa gaacaaggag cgatccgctc cgccgctctc gaacgtgaaa atcgggcgat
     2341 gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga actgctcctt
     2401 tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag cgcgccagta
     2461 tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc caggaaccgg
     2521 caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta aatccgtatt
     2581 gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga atagcggtac
     2641 cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga tcgatgagat
     2701 cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg tcgatcgccg
     2761 ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag agatgaccaa
     2821 gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttgt gggtgatctt
     2881 caactgggat agctaccgta gccgtgtaac aggtaaagag tattaatccc cgtttctggc
     2941 ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactgtta aacatcttct
     3001 gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg cgtcctggaa
     3061 agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagtacga cacaggaagg
     3121 cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg ccttaagcgc
     3181 agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa agctggcttc
     3241 ggtaaccgat gccgaaaaca tcaagaacca gatgctggaa aagctgggcc tgaacagtga
     3301 agagcaaaaa gaagacacca actatctgga cggcattcag ggtttgctga aaaccaaaga
     3361 tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa aggtgaaaac
     3421 caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa ccgcgaaacg
     3481 ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc ctgctcctgg
     3541 acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt ttataaacta
     3601 attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc gacgccatta
     3661 cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt gaggatcgcc
     3721 cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt tacgcatgga
     3781 aagcgggacg taacacctgg tggtttgcag ccacattaac ggtgctgggg ctatttgtcg
     3841 ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt aacgacgcgg
     3901 ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac atcctgccgg
     3961 gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc gctgggctgg atcctgcgcc
     4021 gtcgtcgcca tcatccgcac cattttggtt acagcctgct ggcgctctta ctggcgctgg
     4081 gttcggtgga cgccagccca gcatttcgtc agataacgga actggtgaaa tcccagtcac
     4141 gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact atccctgacc
     4201 cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat tttgataacg
     4261 aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc ctggatttca
     4321 gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg gcttctcagt
     4381 gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc cgcctctgtc tccagcttct
     4441 tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag aactatttcg
     4501 tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt cctgaagtcg catggcttcg
     4561 accacttata cggctcagaa gagctgaaaa gcgtggtggc cgacccgcac tatcgcaacg
     4621 actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt gaagagcttt
     4681 cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat cacccggatg
     4741 gttttatctc gcgtacctgt aaccgcaaaa aatatgattt tgacggtaag ccgaaccagt
     4801 cattcagcgc ggtaagttgc agccaggaga atatcgcggc gtttatcaac aaaatcaaag
     4861 cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc tgaccattta gcgatgaaca
     4921 acacggcgtg gaaatacctt aataagcagg atcgcaataa cctgtttttt gtcattcgtg
     4981 gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa cacgatggat aacggcgcga
     5041 cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc agtttatccg
     5101 ggcagtcaat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg tggaagccgg
     5161 atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc gaccagcaga
     5221 aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg cgggtttcag
     5281 acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt ttccagctgg
     5341 ccgatttcgc tccacgcgac aatttcgtct gggttgaccg ttgctataag atggcacaac
     5401 tctgggctcc ggaactggca ctctccaccg actggtgtgt ctcgcaaggg cagcttggcg
     5461 gtcagcaaat tgttcagcat gttgacaaaa caacatggca gggcaaaacg gcatttaaag
     5521 atacggtcat cgacatggcg cgttataaag gcaatgtcga tacgctgaaa attgttgata
     5581 acgatattcg ctacaaagcc gacagtttca tcttcaacgt cgccggtgcg ccagaagagg
     5641 tgaaacagtt tagtgggatt tcccgtccag agtcgtgggg ccgctggtcc aacgcgcagc
     5701 tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt gacctggtga
     5761 ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc gtaggcaatg
     5821 aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat ttcgataacc
     5881 caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc aacgaaggga
     5941 atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt aaagttgtgg
     6001 aacgtgaagg gtaatttctg actgatgtgt aggtcgcata aggtgcgacc tacacatctt
     6061 ctccggccta caaaacatca ttgagaaaca gaaaaaaaac agcgcccaac acttccccgg
     6121 acgctgcatg gcaactttaa taatctcgcc ttatcagaaa gtaattacac ccttaatcaa
     6181 ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga actgatagcg
     6241 atgggttaac atcatgtcgg cagtgatttt tccttccgcc atcagtcgac cgactttagc
     6301 aaaatcttcc ggcgtggcgt tgcggctgcc catcatcgtc gtttcttttt tatggaattc
     6361 cggatcggag aactgcaact cacctttaaa caggccgaca aataccaccg tgccgccgtg
     6421 acgaatcaaa ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt cgatcacttt
     6481 ctgcgccagc gaaccaccaa actgcgcccg tagctgcgcg tcaaaatctt cggctgacgg
     6541 gtccagtaaa ggtaattcca gacgcgttgc cacatgttca cggcgcgccg gactggtatc
     6601 cgccaccacc acctgtgcgc catcggcttt agcgattgcc gccgcgccca aaccgattgg
     6661 ccccgccccg accaccagca cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg
     6721 agcgctaata gcgaaaggtt caatcaatgc ggccgcctgc gggtcaatac cgtctgcggg
     6781 caaaatgttc gccaccggca cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc
     6841 aatgactgaa attttttcac agcaattggt acgcccgctt ttacacgccg ggcattgctg
     6901 acaggcaaca taagggatca cagcaacttg ctgaccattt ttaagatcag caatattttt
     6961 acccagccca acaatctccc cacatatttc atggcctaaa acacgtggat aactaaaaaa
     7021 tggttgattt ccaccccagg catgaatatc ggtaccgcaa atcccgacag acttaatttt
     7081 tattaatgct tcattgtcac ccggaatagg tatctcgcgt tgtttccaga ctaattcttt
     7141 cggctgctgg caaattaaaa cattcatcgt agacatgatt cggctctcca ttttttgttg
     7201 ccttagatat caatgaaaat gaaaaatggc agcggaacgg aaaatctgtt ttgtgaaaac
     7261 acacgcataa aaatgtttta aatcgggttt taatggactc acaaggagga aaatatgagt
     7321 cgttcacaaa atttacgcca caatgtgatt aaccaggtca ttgatgatat ggcgcggggt
     7381 catatccctt ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc
     7441 acaacagtgc gtcatattct cagccactta cgcgaatgcg gcgtcttaac gcaggttgga
     7501 aacgactatg ttattgcgcg taaacctgac catgatgacg gtttcgcctg tactaccgct
     7561 tcaatgagtg aacaaaacaa agtgtttgag caggcttttt tcacgatgat aaaccagcgt
     7621 cagttacgcc ctggggaaac tttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc
     7681 ccggtggttg taagagaata tcttttaaaa ttcggccgtt ataatcttat tcaaagcgaa
     7741 aaacgcggcc agtggagcat gaagcaattc gatcaatcct acgccgagca gctgtttgag
     7801 ctacgcgaaa tgctcgaaac acattcactt cagcactttc tcaacctgcc tgatcatgat
     7861 ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg tgacaacatc
     7921 ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt actgctttca
     7981 gctgccgaca atatcttttt tgatcaatcg cttgagatca tctcagtcat ctttcatttt
     8041 cactatcaat gggacgaaag cgatcttaaa cagcgcaata tcattgccgt cgatgagcat
     8101 atgaccattc tcagcgctct gatttgccgc agtgaccttg atgccaccct tgcgctccgt
     8161 aaccatttga attcagctaa acaatccatg attcgatcca ttaatgagaa cacgcgttat
     8221 gctcattaag tacggattaa aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag
     8281 ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa aaattttttt
     8341 gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atgtcaacct tgcgtaattc
     8401 acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat
     8461 caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc
     8521 agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt
     8581 attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt
     8641 aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt
     8701 ttcactcgct tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc
     8761 acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcaa tgtctggcat
     8821 ggtgcacaac tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc
     8881 gatgaaccca tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg
     8941 cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct
     9001 ggcggcgatg atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg
     9061 gatttttctc gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac
     9121 tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag
     9181 ttttgccgaa tggcgcagcc tgttccgtaa ccgtacaatg tggggaatga tgctcggatt
     9241 cagtggcatc aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac
     9301 agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg
     9361 ggctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc
     9421 tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac
     9481 gctgatagta ccacaagcga caacatccat gacggcggtt ctgctgattg gcatggcact
     9541 gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc
     9601 tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt
     9661 tgcgccgatc attactggtt ttattgttga taccacccac tcattccgtc tggcactaat
     9721 catctgcggt tgcgtcaccg cagcgggggc actggcgtac atcttcctgg ttcgtcagcc
     9781 gatcaacgac ccacgtaaag attaatctcc ttatgcccga taacattttg cttatcgggc
     9841 attttcatgg cgattaaaat gtttcccagt tctcctcgct atctgccacg gccattttac
     9901 gcggcgcagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgct
     9961 gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccagcg
    10021 cggcggcggc agcggcagat tcttccacca gcgcggcgtt ctgttgagtt acccggtcca
    10081 tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag
    10141 caatttcgcc cataatgtcc gtcacgcggg tcacggcgct gacaatctcc gccattgttt
    10201 ccccggcgct ttcgaccagc gtagagccaa catccacttt ccccaccgag tcttcaatca
    10261 ggcttttaat ttcacgagcc gcctgagcgc tacgctgggc cagattacgc acttctcccg
    10321 caaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc tgcttcaacc gccgcgttca
    10381 aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt
    10441 tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc actttgccgc
    10501 cccgttgcgc cgtttcagaa gcacttaatg ccagatggct ggcctggcgc gcattctcgg
    10561 cgttctgttt caccgttgcg gtcagttgct ccatgctggc tgccgtctct tccagcgaag
    10621 cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt tcgctggcac
    10681 cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct
    10741 gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg
    10801 gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga ttcattggcg
    10861 ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt acgacgatca
    10921 tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg ctgacggcga
    10981 tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtcct
    11041 gatatccctg agtcggctga tcaaagaact cgttgatttt gcctgcacct aacagttgga
    11101 tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg
    11161 cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt ttttccgcct
    11221 gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg ctaccaatat
    11281 tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca
    11341 acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga
    11401 aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa
    11461 ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat cttgatacgt tttaacatgg
    11521 tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa ctttatgaaa
    11581 aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt atcagatact aaaaagttat
    11641 tcattatcgc ccgggatgtc ttcttgtcgg gaattgccca gagcaataaa ttcctctaac
    11701 aaatgggtta actgttgcat tttctcggca gtgaactgag cttcaatctg ccgataggct
    11761 tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt cagcgagata
    11821 tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc gcgttccatc
    11881 cgcgtaagga tcccggtcag gcttgggcgc aaaatgcagg cgcgatacgc cagatcgtga
    11941 aaatccattg atgggctctc cgccagaata cgcacgatgc gccactgttg ctcagtcaaa
    12001 ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc ctggagcaac
    12061 gcaatggtta gagagtcgtg cattatcttt cccctgaaaa attcaaaatc attaatatag
    12121 aaacagttaa tcatcacgtt accgggactg cgctgcccct gcaattcttc attttatcaa
    12181 aattgagcat gttttgttaa cttgctgata tttaatattt tattatcttt aaatgtaaat
    12241 agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc aatcgggaat
    12301 aaaagtaatc attaacatat taatgattaa gatgatcgaa tccgaggagt ggtaatgaaa
    12361 ggcactatct tcgccgtagc attgaaccat cgcagccagc ttgatgcatg gcaggaagcg
    12421 ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat taaaccgcgc
    12481 aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa ggtactgagc
    12541 ggtgcgacag ttgcgctgat tgtgggaaaa acggcgacga aagtacgtga agaagatgcg
    12601 gcagagtaca tcgccggata tgcgctggct aacgacgtca gcctgccgga agagagcttt
    12661 taccgtccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg cgaaaccgtg
    12721 gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg tcctgccgat
    12781 cactggaaca ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa
    12841 tttgccacac tgaatccagg cgatgccatt ctgctcggca cgccacaggc gcgcgtggaa
    12901 atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg
    12961 gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgcagcc acacccgcac
    13021 ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact ggaatttaag
    13081 ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg cgataaccag
    13141 acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct ggtggtagtt
    13201 attggcaaac aggcgcgtag cgtcagcgaa gccgatgcca tggattatgt cgcgggctac
    13261 accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg ccctaacctg
    13321 cgggtaaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg
    13381 atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt acgccagcaa
    13441 ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt aagcgaattt
    13501 atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt atctgacgtg
    13561 gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt
    13621 gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg gatcaacggc aaaaatgttg
    13681 caggtaacga ctacttccag accaccaatc cggcaacggg tgaagtgctg gcggatgtgg
    13741 cctctggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag gcgttcccga
    13801 aatgggccaa tctgccaatg aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga
    13861 tcgaccagaa cgtgccagag atcgccgcga tggaaaccgc ggacaccggc ctgccgatcc
    13921 atcagaccaa aaatgtgttg atcccacgcg cttcccacaa ctttgaattt ttcgcggaag
    13981 tctgccagca gatgaacggc aagacttatc cggtcgacga caagatgctc aactacacgc
    14041 tggtgcagcc ggtaggcgtt tgtgcactgg tgtcaccgtg gaacgtgccg tttatgaccg
    14101 ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg
    14161 aactctcccc gctgaccgcc gaccgcctgg gtgagctggc gctggaagct ggtattccgg
    14221 cgggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggcgatgcg ctggtccgtc
    14281 atcatgacgt gcgtgccgtg tcgttcaccg gcggtacggc gaccgggcgc aacatcatga
    14341 aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg taaatcgccg gtgctgattt
    14401 ttgaagatgc tgatattgaa cgcgcgctgg acgccgccct gttcaccatc ttctcgatca
    14461 acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc tacccggaat
    14521 tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga
    14581 atacccaggt tggcgcgctt atcagccagc agcactggga aaaagtctcc ggctatatcc
    14641 gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc
    14701 tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca gacgttgata
    14761 accgtatgcg agtcgcccag gaagagattt tcgggccggt cgcctgcctg ctgccgttta
    14821 aagacgaagc cgaaggctta cgtctggcaa acgacgtgga gtacggcctc gcgtcgtaca
    14881 tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa gctggcatgg
    14941 tgttcgtcaa cacccagaac gtgcgtgacc tgcgccagcc atttggcggc gtaaaagcct
    15001 ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg
    15061 tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat gggtaagtta
    15121 gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact gccagggaaa
    15181 aaccacggtt gccgccaggg cgcgatcgac gggcataaag agatcagcaa gcgttgccgg
    15241 gaaatgggcg tcgataccat tatcgttttc gatacccact ggctggtcaa cagtgcttat
    15301 cacatcaact gtgcagacca ttttgaaggc gtctacacca gcaacgagct gccgcatttt
    15361 atccgcgata tgacctacaa ctacgagggc aacccggagt tggggcagct tattgccgat
    15421 gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa
    15481 tacggcacgc tggtgccaat gcgctacatg aatgaagaca agcacttcaa agtggtttcc
    15541 atttcagctt tctgcactgt tcacgatttt gccgacagcc gcaagctggg cgaggcgatt
    15601 ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg
    15661 caccgcttta ttgacgatca gcgagcggaa gaagggatga acagctacac ccgcgagttc
    15721 gaccgccaga tggacgagcg tgtggtgaag ctgtggcgcg aaggccagtt caaagagttc
    15781 tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat gcacgacacg
    15841 gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga gtttatcacc
    15901 gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct tcccgcgtaa
    15961 ggacgtttta tgccgcactt tatcgttgaa tgcagtgaca acatccgcga agaagccgac
    16021 ctgccggggt tgttcgccaa agtgaatccg acgctggcag ccacgggtat ttttccgctg
    16081 gcgggtattc gcagccgcgt gcattgggtc gatacctggc agatggccga cgggcagcgt
    16141 gattatgcct tcgtgcatat gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgt
    16201 cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc acttcgccgc cctgatggag
    16261 agccgcctgc tggcgttgtc gtttgagatt gaagagctgc atccgacgct gaattttaaa
    16321 caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt gcccggtggc gctgcgctta
    16381 ccgggcctac aaaaccccaa accgtacacc gtaggccgga taaggcgcag ccgcatccgg
    16441 caatgccaca ggatatcgct atgttcgata aacataccca caccctgatc gcccagcgtc
    16501 tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcactggat tacccggaga
    16561 tcaccatcga agacgcttac gcggtgcagc gcgaatgggt tcgcctgaaa atcgccgaag
    16621 gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag gcaagctcgc
    16681 agatcagcga accggattac ggcgcgctgc tggatgacat gttcttccac gatggcagcg
    16741 atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg
    16801 caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt ctacaacgcc acggactacg
    16861 tgatcccggc gctggagctg atcgacgctc gctgccacaa catcgatccg gaaacccagc
    16921 gcccgcgcaa agtgttcgac accatttctg ataacgccgc caacgccggg gtgatcctcg
    16981 gtggtcgtcc cattaagccc gatgagttgg atctgcgttg gatctccgcc ctgatgtatc
    17041 gcaatggcgt gattgaagaa accggcgtcg ccgctggtgt gctgaatcat ccggcaaacg
    17101 gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa gccgggcaaa
    17161 tcattctcgg cggttcgttc acccgcccgg ttccggcgcg taagggcgac accttccacg
    17221 tcgattacgg caacatgggc tccattagct gccgctttgt ttaaggagag aacgatggaa
    17281 aacagtttta aagcggcgct gaaagcaggc cgcccgcaga tcggattatg gctggggctg
    17341 agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt attgatcgac
    17401 ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc
    17461 taccccagcc agccggtggt acgtccgtcg tggaacgatc cggtgcaaat caaacaactg
    17521 ctggacgtcg gcacacaaac cttgctggtg ccgatggtac aaaacgctga cgaagcccgt
    17581 gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg cagtgcgctg
    17641 gcccgagcct cgcgctggaa tcgtattcct gattacctgc aaaaagccaa cgatcaaatg
    17701 tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca gattctggac
    17761 gtggaagggg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga tatgggttat
    17821 gccggtaatc cacagcaccc ggaagtacag gccgcgattg agcaggcgat cgtgcagatc
    17881 cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc aaaacgctat
    17941 ctggaactgg gcgcgctgtt tgtcgctgtc ggcgttgaca ccaccctgct cgcccgcgcc
    18001 gctgaagcgc tggcagcacg gtttggcgca caggccacga ccgttaagcc cggcgtgtat
    18061 taatgccgga tgcgctgcac ttatccggcc tacactcgca ccaaaccgta ggccggataa
    18121 ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg cggaccggat aaggcgtttg
    18181 tgcgcatccg ccataaacct tgtcgtaccc tacaaaaatc ccattagagg aagaaaaatg
    18241 agcgacacct cacctgccat accggagagt atcgatccgg cgaatcagca taaagcgctg
    18301 actgccggac aacaggcggt tattaagaag ctgtttcgcc gcctgatcgt ctttctgttc
    18361 gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg gctttgccgg actcacgatg
    18421 ggacgcgacc tcggtctgag cgccaccatg tttggcctcg ctaccaccct gttctatgcc
    18481 gcttatgtca tcttcggcat tcccagcaac attatgctga gtattgtcgg tgcgcggcgc
    18541 tggatcgcca ccatcatggt gctttggggc atcgcctcta ctgccaccat gtttgccact
    18601 ggccccacca gcttgtacgt actgcgtata ctggttggca ttaccgaagc cggctttctg
    18661 cccggcattc tgctgtattt aaccttctgg tttccggcct atttccgcgc ccgtgccaac
    18721 gccttgttta tggtagcaat gccggtaacg acagcgttgg gctcgatcgt ttccggctac
    18781 attttgtcgc tggatggcgt catggcatta aaaggctggc agtggctgtt tttgctggaa
    18841 ggcttcccgt cggtattact cggcgtcatg gtgtggttct ggcttgatga ctcaccggac
    18901 aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc aagagatgat ggataacgat
    18961 cgtctgacgc tggttcagcc agagggagcc atcagccacc acgccatgca acaacgcagt
    19021 atgtggcggg agatcttcac tccggtggtg atgatgtata ccctggcgta tttctgcctg
    19081 accaacacac ttagtgcgat cagcatctgg acaccgcaga tcctgcaaag ttttaatcag
    19141 ggcagcagta atatcaccat cggcctgctg gccgccgtac cgcagatttg taccattctc
    19201 gggatggtct actggagccg tcactcagat cgccgccagg aacgaaggca tcacaccgcc
    19261 cttccttatt tgttcgctgc cgcaggttgg ttactggctt cggcaactga tcacaacatg
    19321 atccagatgc tggggatcat tatggcttcg accggatcat tcagcgcaat ggcgattttc
    19381 tggacaacac cggatcagtc catcagcctg cgggcacgag cgatcggtat tgcggtgatc
    19441 aacgccactg gcaacattgg ttcagcgtta agtccgttta tgatcggctg gttgaaagat
    19501 ttgaccggca gctttaacag tggattgtgg tttgttgccg cgctgctggt gattggtgcg
    19561 gggattatct gggcgattcc aatgcagtcc tcccgtccgc gagcgacccc gtaaggaacg
    19621 acgatgtgtg accgtcagat tgccaatatt gatatcagca aagagtacga tgaaagcctg
    19681 ggcacggacg atgtgcatta tcagtccttc gcccgcatgg cggccttttt tggccgccat
    19741 atgctgccac atcgccacga acagtacttt cagatgcatt tcctcaatag cggacagatt
    19801 gagctacaac ttgacgatca tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg
    19861 ccgtcagtac ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta
    19921 cgggaagatc tgatctggcc cctgctggaa gttctttatc cgggcactag ggaaaccttc
    19981 ggcctgccgg ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa
    20041 cactattggc aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc
    20101 ctgacgttac tggcacaggc agtgttcacc ctactgctgc gtaacgcaaa actcgacgac
    20161 catgccgcca gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt
    20221 gaaagccatt ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc
    20281 gaatcacgac tcacggacat ctgccgtcgc tttgccaacc gtccgccaaa acggttgatt
    20341 ttcgacaggc agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac
    20401 aatattgcct ggcaactcgg ttttaaggat ccggcttatt ttgcgcgctt ttttaatcgc
    20461 ttagtcggtt gctcgcccag tgcttatcgc gccaaaaaag tacctgtgac gtgaaaatcc
    20521 cttagcctta acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag
    20581 gggatttagt aacgtcaaaa atcgctaaaa gcgacctcga tcacaaaacc gtacccgacc
    20641 tgaaaagtac cagccattgt cccaaaagtc tcttcccgta atcgcccctg caagcttcaa
    20701 ttgaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt
    20761 cgacatgaaa ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta
    20821 tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt
    20881 cactactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct
    20941 acacaaaccg gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta
    21001 tacccataaa ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgcgatgc
    21061 catcgctgag tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc
    21121 tgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc
    21181 ccgtaactgg tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa
    21241 cccaccgatc gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga
    21301 aaaagagact gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc
    21361 gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga
    21421 cttcgcactg atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc
    21481 ctcttatgag atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg
    21541 cttcgatgag aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt
    21601 actgatctac cgcgatttcg atcgctgccg tcgctggacg atggaaggcg gttttgcccg
    21661 tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctagacttca ttacggcact
    21721 gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtgc aggccgatct
    21781 cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga
    21841 agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg
    21901 cgtactggca ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag
    21961 tggcctgatc tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta
    22021 tctggcgaag tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct
    22081 caaactgatg tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat
    22141 caactactcc ggtagccagg atgagattcg cctgcagtgt ctgcgccagg cacaaagctc
    22201 cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa
    22261 cggctggact gtgccgcacc tgcacaacaa cgacgatatc aacatgctgg ataagctgct
    22321 gaaataacgc agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac
    22381 gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa
    22441 tgcgggatta cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg
    22501 tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggcaa gttgtgcgtc
    22561 aacgtcctca accatgagca ggaactgatg gcacgccact tcgcgggcat gacgggcatg
    22621 gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg
    22681 ctaaaaggtt cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca
    22741 catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt
    22801 atctacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct
    22861 ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca
    22921 cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta catccctaaa
    22981 ataaccactc agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag
    23041 gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga
    23101 ttaacaaatt actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg
    23161 gagcttacaa cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa
    23221 agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac
    23281 cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg
    23341 gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt
    23401 atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga
    23461 cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt
    23521 ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg
    23581 cacagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg
    23641 cggttcagga ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg
    23701 agatgatcaa agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct
    23761 taatcatgat catcatcctc gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa
    23821 gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct
    23881 acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc
    23941 tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac
    24001 tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcac
    24061 tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg
    24121 gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg
    24181 cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg ttcccgttcc
    24241 tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc tcttccggta
    24301 cgacgccaaa actgctggct aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc
    24361 tgatggagtc cttcgtggcg attatggcgc tggttgcagc gtccatcatc gaaccgggtc
    24421 tttacttcgc gatgaatacc ccgcctgctg gccttggcat caccatgcct aacctgcatg
    24481 aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg
    24541 cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg
    24601 cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg
    24661 taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc
    24721 acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtactcgtt
    24781 ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg
    24841 actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc
    24901 tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta
    24961 tttccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga
    25021 tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca
    25081 ccacctgggc gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct
    25141 acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga
    25201 ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcgggcctg agtattctgt
    25261 tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta
    25321 acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga
    25381 agatctcttc gcaccactaa ccgtatttag ccccgcttcg gcggggcttt gttcttatca
    25441 gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag
    25501 atgttgattg gcattccaga ctatgacaac tacgtcgagc atatgaagac caaccatccc
    25561 gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaacgc acgctacggc
    25621 ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt
    25681 taccctactc accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa
    25741 tgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga
    25801 tgatcaattg attggtgatc gcgcaacgca gatcaaaacg ctgaccaacg gctgcatctg
    25861 ctgttcgcgc tccaacgagc tggaggacgc gctactcgac ctgctggata atctcgacaa
    25921 gggcaatatt cagttcgatc gtctggtcat tgaatgcacc ggcatggccg atcctggccc
    25981 gattattcaa acctttttct cccatgagat tttatgccag cgctacctgc tggacggcgt
    26041 gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca
    26101 gtcccaggtg ggctacgccg accgtattct gctgactaaa accgacgtcg caggcgaagc
    26161 agaaaaactg cgtgaacgcc tggcgcgcat caacgcccgt gcaccggtct acaccgtcac
    26221 ccacggcgac atcgacctgg gcctgctgtt caacaccaac ggttttatgc tggaagaaaa
    26281 cgtcgtcagc accaaaccgc gtttccactt tatcgcggat aaacaaaacg atatttcgtc
    26341 gattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa
    26401 cctgctgctg gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg
    26461 cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggacag
    26521 gccatggggc gatgaaacac cgcatagcac gatggtgttt atcgggattc agttgccgga
    26581 ggatgagatt cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa
    26641 tacctgagac tttatcaccc tcatattcct ccagaatatg agggctttcg ggcttaatta
    26701 tctctgaacg tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa
    26761 ccagagcgac ttacggccaa aattttcgta cattttcagc tgatcttcat agtgcttatc
    26821 aactgttcca tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag
    26881 cacaggacga tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt
    26941 ttgatactcc gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt
    27001 atttgcccgg aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata
    27061 gcgtttggaa agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg
    27121 tttcccagca aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc
    27181 atacaaaatt tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt
    27241 ttcgtagcca ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt
    27301 acgcttcaac atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt
    27361 tttaccatca tcattaagca aattgatgcc atcccaacgt gttaatgttt ctaccaactg
    27421 acgacgcgga tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag
    27481 cctcaggtta agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt
    27541 taagcgtggc ttttgctcaa gcagtcggtc gatctccgta acgcgatctg caccacccca
    27601 caaaaaggca aacagatctg aagcgggata atctttttgg ggagaattgt tccagttagc
    27661 aatatatccc gactgggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
    27721 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
    27781 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
    27841 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
    27901 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
    27961 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
    28021 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
    28081 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
    28141 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
    28201 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
    28261 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
    28321 gattgctttc gcatcctggg ctttgctttt gccgatcacc cacatattgc tggtcgttgg
    28381 ataccccgcc agaccattgg caccaccctg tgcaaattgt gcagcaatag tttcccggtt
    28441 cttccctgct gttaacgcca gcagtgcgcc atccgcccct tttgctggtc ggtcaagcat
    28501 tggtgcaggt aaatcgtagc gtggcaacag agctgctgtt tgcgagtttt gctgattaaa
    28561 tttaagtggg tagttactct cttgtacggc aatagtggtt ggcgctgatg ggtttaccag
    28621 ccatttcaac tgattaaata ccgccatgcc ttgtgataca ccatatttat cttttaaagc
    28681 cgttagcagt gccagattat caatttcgct agtgctatca gagaagcggt ttgccatggt
    28741 gcccacaaat atcatcgcga catcaaacgg ttcccagcgc ttaggagtaa agccaaatgt
    28801 attaaactgt tttggtaaga gcgtctctgg attggtattt accttatcaa tccaggcatt
    28861 cattccatca gcgtagcctt gcagaatgga catatcctct ggggaaaggg cagcaatttg
    28921 cgcccggata gcatccggcc agtagttacg acggatatct ttatcaaatt tcacaaaatc
    28981 tttgccaagc acttccgcga cagtcccttg agtactgcga cgtgccattt ccatctgaaa
    29041 aaggcgatct tgtgctacta catagccata gccataaaat aggtgccatg tatcattggc
    29101 ataaatatgc ggcatgccgt attcatcgcg aacaatcttt atctcacttg acgactgctc
    29161 agccagtgca ggtaagctcc aataatacat cagggaagca gtaacacagt tcacgatcat
    29221 acgatttcta tttttcattg tatcctctgg caggtgtata attagcgaat tgatactagc
    29281 aacgaagctt cggttatcta tttgcagaga attgtttcat tatgaactgt gagcgcgata
    29341 actaattttt gatacaaaaa tttcattaat gtgatctaaa acgaatcgct ttattcaccg
    29401 ctgatgattt tgttctattc ctttttgtga cagatccccc ggacgcatag atgatgaccc
    29461 ccagagagac agctcaggtc attatctgta tgtaaatcag aaaaatatgt tgtaaacgac
    29521 ttttatggtt ggattttgta gcaattttga atgcagacag gcagcatttg ccaacacgcc
    29581 gcccatagtt cactccacat cctccctgcg taacccaata tccctcaacc gttcatcact
    29641 catctgctgt aacactcgtc gggtctgcat ttgccgccgc caacgcctca ccgcatgcca
    29701 gagttgcaca agtctgataa acggcgtttt ggctttgttt tcgtgaaatt ccatctgctg
    29761 cctcctcatc tttcagaggc ttcatcatcc gccttctggc agttcacaat acagatgcaa
    29821 atattagttt tatttaacat acagacaggt taaaacagca tctgcatccg gttttttagc
    29881 ctcatctgta ccggtttttc tttctgtatg ggccaaataa ggaatacagc atgacgcgtt
    29941 atcaacatct ggcgactcta cttgccgagc ggattgagca agggctgtat cgtcacgggg
    30001 agaaattgcc gtcggtgcgc agcttaagtc aggagcacgg cgtcagcatc agcaccgtgc
    30061 aacaggcgta tcagacgctg gaaacgatga ggctcatcac tccgcagccg cgttcgggtt
    30121 attttgtcgc acaacgtaaa gcccagccgc ctgtaccgcc gatgacgcgt ccggtgcagc
    30181 gcccggtgga aattacccag tgggatcagg tgctggatat gctggaggcg catagcgaca
    30241 attccattgt tccgttaagc aaaagcacgc cggatgtcga aacgcccagc ctgaaaccgc
    30301 tgtggcgtga gctaagccgg gtggtgcagc ataatctgca aaccgttctc ggttatgact
    30361 tgttagccgg tcagcgagtg ttgcgggagc agattgcccg cctgatgctc gacagcggct
    30421 cggtggtcac cgccgatgac atcatcatca ccagcggctg ccataactcg atgtcgctgg
    30481 cgttaatggc ggtgtgtaaa ccgggcgata ttgtcgcggt cgaatctccc tgttattacg
    30541 gttcgatgca gatgctgcga ggcatgggcg tgaaagtgat tgaaatccca accgatccag
    30601 aaactggcat cagcgttgaa gcactggaac tggcgctgga acagtggccg attaaaggca
    30661 tcattctggt gccaaactgt aataatccgc tgggatttat tatgccggac gcacgcaaac
    30721 gggccgttct ctctctcgct cagcgtcatg atattgtgat ttttgaagat gatgtctacg
    30781 gcgaactggc aacggagtat ccgcgcccgc ggaccatcca ttcatgggat atcgacgggc
    30841 gagtgctgtt gtgcagctcg ttcagtaaaa gtattgctcc aggcctgcgc gtgggttggg
    30901 tcgcaccggg gcgttatcac gataaactga tgcatatgaa atacgccatc agcagcttta
    30961 atgtgccgtc cacgcaaatg gcggcggcaa cgtttgtgtt ggaaggccac tatcatcgcc
    31021 atatccggcg gatgcggcag acttatcagc gcaatctggc gctttatacc tgctggatac
    31081 gggaatattt tccctgcgaa atctgtatta cgcgcccgaa aggcggattt ttactgtgga
    31141 tagaattgcc tgaacaggtc gatatggtct gcgttgcgcg gcagctgtgc cgcatgaata
    31201 tccaggtggc agcaggctcg attttctcgg cttccggcaa ataccgtaat tgtctacgca
    31261 tcaactgcgc tttgccgctc agcgaaacct atcgcgaagc actaaagcaa attggcgagg
    31321 ccgtgtatcg ggcaatggaa taaattcgaa ttagtggcga agcacctgat ttcctctcac
    31381 gaatcgcctg taactcgttg tatctttgca gggtcatcgc cacgttccag catggcttag
    31441 gcaaagtgtg cttcgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaaccg
    31501 cgcaaattca atagattgca gaggaaatgt aggtctgata agcgtagcgc atcaggcagc
    31561 tttgcgtttg tcatccgtct ctgattagta ttcgatccat ggatcgcgct tgatttcagg
    31621 caagggctgc tctaatgcct gcatttgctc tgtcgaaagt ccggtaatct tttgtatcca
    31681 ttcaggatct gccccattct gcaacaacaa tctaaggagg cgttgttccc cttcttcttt
    31741 ccccttttcc atccccaaca gccatccatc attataaatt cgctctgcaa tcgtcattat
    31801 tcgctccctg tgttgtggca ttcgacgggt aagttcgctg ataaacgcct taaaacgcgc
    31861 ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt cattagcaca
    31921 ctctgtaacc agtaatacta ccagttggtc aataagcccc atcagatcgc gctggcgaat
    31981 atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct cgtcgtctgg
    32041 cacgacagtg acatccacca gcgggaacgc tgcgctataa agtttccgtg cggtagtcgg
    32101 ggcggcaaat tcgtccagcc agcacaggga ccaggggtaa ggactacggc taccgtgata
    32161 aaacagcatc gggataacca gcggtagcgg ccggcgttta tcatgctcga tatggcgctg
    32221 catcaccgcc atggaatatc gcatcaggcg aaacgccata tgaatatcct cgcggctctg
    32281 atgttcaatc actacgtaaa tataaccatc accttcacgg gtctttaccg accacagaat
    32341 atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca gttttaagct
    32401 gtcgagatcg cacagttcac gtaaatcttt aggtaagtgg atctccataa aatcccgcgc
    32461 ggtgccaggg tgcgtgagaa aggacttaaa taatgcgtca tgcggcgtgc tggtcgagaa
    32521 gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg gctaacaaaa
    32581 ccattctcac ccggcaattt cccgttcccc gaggcgcttt ccaggctatt tttattcttc
    32641 tgcaaacgat acattttttc ggaaacaggc tcgcgtttta catttcctgt acccggcaga
    32701 tattcccctt tccaccgcgc tcttttgatt aaccgcaaat tttaattaca cttaaggtgt
    32761 atattttcta tgcaacccat caattaaaga ggtgtaatgt gctgatgact atttgtaatc
    32821 gttatacatt ctgacctgga gtcagaaggt atttctctgt ctgtgtgttc acaggcagtg
    32881 tggttgatta catgaaattc agtacatttg tccttctcgc ctgcctttcg tcattaccga
    32941 cggtattgaa tttcgttttc cccgttgggg ttctccggac aaggagttgt ttgttatgcc
    33001 acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga ttttgtatga
    33061 ctttgccgcg tacctgtcga cggatctgat ccagccgggg atcattaatg tggtcggtga
    33121 ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg cgggcggtat
    33181 ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc cggtgctgat
    33241 taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa cgtctatgac
    33301 acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg ccaccgttgg
    33361 ttatgtcacg gtgcaggagg cgttcggcca gacaaaaggg atcaagttga tggcgattat
    33421 cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag ctctgatgca
    33481 ctttgtgcac tggaaagtcc tttttgccat cattgcagtt atgggtttta tctcatttgt
    33541 tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat ttagcgccaa
    33601 aagcgtcttt cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc
    33661 aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc cggtgatcct
    33721 gatcgacgcg ggcggcttaa caacttcgca gttcgcctgg acacaggttc cggtgttcgg
    33781 cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga ccgaatcgcg
    33841 ctttatctgg cgtgctgtac ccattcaact ggtcggcctc gcactgttga ttatcggcaa
    33901 tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt atgctttcgg
    33961 gattggtttg atcttcccga ccttattccg ctttacgctt ttttccaata acttaccgaa
    34021 agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg tctcggtcga
    34081 aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt
    34141 ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc gccaacatca
    34201 ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca ggctctattc
    34261 ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac
    34321 tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga
    34381 gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc gctggcggac tggtttgcgg
    34441 tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc
    34501 cgcgtaataa agaccggatt ggcgaaaatc tcggccagtt cgtgcaggaa aaatttctcg
    34561 atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgctg attggcaact
    34621 ggtttagcca gccagaaaac gcccgccgcg ttggtcagca tctgttgcag atcatgagtg
    34681 gttttcttga actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc
    34741 gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag agtatgacca
    34801 aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc
    34861 agcgcgataa atcgcgcaag tttattgccc agcagattgt tcgctggctg gagagtgagc
    34921 atccactgaa agccaaaatt ctccccaccg aatggttggg cgaacatagc gcggagttgg
    34981 tttctgacgc ggtgaattct ttacttgatg atatcagccg tgatcgtgcg catcagatcc
    35041 gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gttgaaaaac gatccggaaa
    35101 tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct tttaaccgct
    35161 atctcagtga attgtggggg gatttacggg agtggctgaa agcggatatc aacagtgaag
    35221 attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg
    35281 ccgatgatgc cttgcgggcg tcgttaaatg gtcacctgga acaagccgcg caccgcgtcg
    35341 cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa agctgggatg
    35401 cgcgggatat gtcgcggcaa atcgagttaa atatcggcaa agatctgcag tttatccgtg
    35461 tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgttg tcgcagctcc
    35521 cggccttgtt ccccctcggc aatttttaga aatccatgaa caactaagtg gctaaccgcg
    35581 agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt ttcattatct
    35641 cccgtggtac ggggaaggaa aatcatgtca cttatcaccg atctacccgc cattttcgat
    35701 cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc
    35761 attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat ggcagccggt
    35821 gcggttgtgg tttcgctctg ttctacctct gatgaaacca ttgaagaagc ggagaaagat
    35881 ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa aaccgataaa
    35941 tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag
    36001 aaaatgtatg aatacatggc ggagtttaag cccgttcatg tgatgcagtt gccgaacagc
    36061 gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg
    36121 gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc cattgcgctg
    36181 aaaaaccgcg aacgtcgcgc actggctaat ttttatcatc ttgggcagtt aaatcctccg
    36241 gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg caaccttccg gttcgataaa
    36301 gaggcgttga tcaatgaact ggacgcgatg acagcccgcg ttcgtcagca gtgggaagaa
    36361 ggccagcggc tggccctgcg tccacgcatt ttaatcaccg gctgcccgat tggcggcgca
    36421 gcagaaaaag tggtgcgcgc gattgaagag aatggcggct gggttgtcgg ttatgaaaac
    36481 tgcaccgggg cgaaagcgac cgagcaatgc gtggcagaaa cgggcgatgt ctacgacgca
    36541 ctggcggata aatatctggc gattggctgc tcctgtgttt cgccgaacga tcagcgcctg
    36601 caaatgctca gccagatggt ggaagaatat caggtcgatg gcgtagttga tgtgattttg
    36661 caggcgtgcc atacctacgc ggtggaatcg ctggcgatta aacgtcatgt gcgtcagcag
    36721 cacaacattc cctatatcgc tattgaaaca gactactcca cctcagatgt cgggcagctc
    36781 agtacccgtg tcgcggcctt tattgagatg ctgtaaggag tggcagtgac atattcgatt
    36841 ggcattgatt ctggttctac cgccaccaaa gggatcttac tggcagacgg cgtgattacg
    36901 cgccgtttcc tcgttccaac accctttcgt ccggcaacag caattactga agcctgggaa
    36961 actctgcgcg aagggttaga gacaacgccg tttctgacgc tcaccggcta tggacggcaa
    37021 ctggtggatt ttgccgataa acaagtgacg gaaatctcct gtcatgggct gggcgcacgg
    37081 tttcttgcgc cagcaacgcg cgcggtaatc gacatcggtg gtcaggacag caaagtgatt
    37141 cagcttgatg atgacggtaa cctgtgcgat ttcctgatga atgacaaatg cgcggcgggc
    37201 accgggcgtt tcctggaggt gatctcgcgc acgcttggca ccagcgtcga gcaactcgac
    37261 agcattaccg aaaatgtcac gccgcacgcc atcacgagta tgtgcacagt gtttgccgaa
    37321 tcagaagtga tcagcctgcg ctcagcgggc gtcgcgccag aagcgattct cgccggagtg
    37381 attaacgcga tggcgcggag gagtgccaat ttcattgctc gtctctcctg tgaagcgccg
    37441 attctgttta ctggtggcgt tagtcattgc cagacgtttg cccggatgct ggaatctcat
    37501 ctgggaatgc cggtaaatac ccatcctgat gcgcaatttg ctggcgcaat tggcgcggcg
    37561 gtaattggtc aacgagtgag gacacgccga tgaaagagtt tttattttta tttcactcca
    37621 cggtcggcgt catacaaacc cgcaaggcgt tgcaggcagc gggcatgact tttcgcgtca
    37681 gcgatattcc ccgtgattta cgcggcggct gcgggttatg catttggctg acctgtcccc
    37741 ccggcgagga aatacaatgg gtgatccctg ggcagactga gtcaatttat tgccagcagg
    37801 atggtgtctg gcgctgcatt gcacattacg gggtttctcc gcgttaagga ttcattaagg
    37861 tcgctacgta tacttctcga tatcttattg atttgcctta aagggattac cgtgacaaaa
    37921 agtatttcgt taagtaaacg catctccgtt attgtgattc tttttgccat tgtggcagtg
    37981 tgtactttct tcgtgcaatc ttgcgcccgt aaaagcaatc atgcagcatc atttcagaat
    38041 taccacgcca ccattgacgg caaagaaatt gcaggcataa cgaataatat ctcttcgctg
    38101 acctggtcgg cacaaagcaa tactcttttt agcactatca acaaacctgc cgccattgtt
    38161 gaaatgacca ccaatggtga ttttatccgc acaattcctc tggattttgt taaggatctc
    38221 gagacgatcg aatacattgg cgataatcag tttgtcatta gcgatgagcg cgattacgcc
    38281 atttatgtga tttcactgac cccaaactcg gaagtaaaaa tcctcaaaaa aataaaaatc
    38341 ccgctgcaag actccccaac caattgcggt tttgaagggt tggcttattc tcgccaggat
    38401 cacactttct ggttttttaa agagaaaaat ccgattgagg tctacaaagt taacgggctg
    38461 ttaagtagca atgaattaca catcagcaaa gacgaggcac tacaacggca atttacttta
    38521 gatgatgtgt ccggcgcaga atttaaccag caaaaaaata cgcttctggt gctatctcat
    38581 gaatctcgcg cactccagga agtaaccctg gttggcgagg ttatcggtga aatatcacta
    38641 accaaaggta gtcgaggttt gtcgcataac atcaaacagg ccgaagggat tgcgatggat
    38701 gcctctggta atatttatat cgttagcgaa ccaaatcgtt tttatcgctt tacccctaaa
    38761 tcatcgcatt aacaggctgg catatttcac ctttttgtta ttaacttgtt aacaacaaaa
    38821 atgacataca aggttaaaca tgccttcgtc ctcgcatccc gtagaacgct tttctttcag
    38881 caccgcgctt ttcgggatgc tggttctgac cttaggtatg ggattaggtc gcttccttta
    38941 tacgcctatg ttgcccgtca tgatggcgga aggggcgttt tcatttagcc agctctcgtg
    39001 gattgccagc ggcaactatg cggggtatct ggctggcagt ctgctatttt cgtttggcgc
    39061 atttcaccag ccgtcgcgcc tgcgcccatt actgttggtt tctgccctgg cgagcgggtt
    39121 gttgatcctc gcaatggcat ggttgccgcc atttattctg gtgttattga ttcgcttcct
    39181 ggcgggtgtc gccagcgccg gtatgctgat ttttggttcg acgctgatta tgcagcacac
    39241 gcgccatcct tttgtgctgg cagctttgtt ttctggcgtt ggcattggca ttgcactggg
    39301 caacgaatat gttctggcag gcctgcattt tgccttctcc tcgcaaacgt tatggcaagg
    39361 cgccggggcg ctttctggca tgatgctgat tgcacttacg cttttaatgc cctcgaaaaa
    39421 acacgccatc gcaccaatgc cattggcaaa aacggagcaa cagataatga gctggtggtt
    39481 actggctatt ctgtacggcc ttgcgggatt tggttatatc atcgtcgcga cttacctgcc
    39541 gctcatggcg aaagatgcag gctcaccatt gttaaccgcc catctctgga cgttagtcgg
    39601 cttatcaatt gtgccaggct gctttggctg gctatgggca gcaaaacgtt ggggggcttt
    39661 gccttgcctg acggcgaatt tgctggtgca ggctatcagt gtgctgctta ctctcgccag
    39721 cgactctcct ctcttgctta tcattagtag tatcggtttt ggcggtacct ttatgggcac
    39781 gacatctctg gtgatgacta tcgcccgcca actgagcgtt ccgggaaatc tcaatctttt
    39841 aggctttgtg acgctcattt acggcattgg acaaatcctc ggcccggcgc tgaccagtat
    39901 gctcggcaat ggaacatcgg cgcttgccgg cgccactctc tgcggcgcgg cggcgttgtt
    39961 tattgctgcg cttatcagca cagtacaatt atttaagctg caagtggtca cttcatgagg
    40021 agtgtttccc ttaccactca agaaactcaa cagcaaccgt tgatgtcagc cagaccccgt
    40081 tttccgcttg ccagaaaggg atgcctcgtt tcgccatttc ctgtgctttg acggttaaga
    40141 taaccggcga tccgtgtctt gctcccactt tgcgtgccgt tgcttcatca gcagagagat
    40201 gtacgtaatg acgctcccct gcaatcagcc cctgtttttt tatctcgtca aggaaacggc
    40261 ttgccgtacc gtggtagaga aactgtggcg gtgttttttc ggcaaaggaa atggcgacct
    40321 gcgaagtcga atgcccctga accgcgcgga tgcacctacc atcactggaa taactaaaac
    40381 gctttttatc gctggtggca accacagtat ccagcaatgc acgggttaac cgcttgcccg
    40441 ctttttgggc acaaagtatg agcttatcga tatccgccca tccttcacgg tcaagtacga
    40501 taccaatcgc ctccggtttg tgtcggagga caaaacttaa aaacttgctc gtatccgcca
    40561 actctttttc gttgtacttt gccatcgcgt tttcgtccct ggattttctg tttttattta
    40621 ctgatgtcat tgtcactggg tttatgaatt ttctgcaact caatcctgcg cagagccttt
    40681 tctacacagc gtaatttttc cgctatgcaa aaaacgcata gcaagaaaca tacttgcatt
    40741 ttccccatac ccacatggct gataaggttt ttcagctcca ggaattttcc tggctcaagc
    40801 aacacacaaa cacaaaaaaa caatggggat tgttatgaca caacaagggg atgctgtagc
    40861 cggggagctg gcgacagaaa aagtgggcat taaagggtat ctggcttttt ttctcaccat
    40921 aatctttttt tccggagttt tttccgggac agacagctgg tggcgcgttt ttgatttttc
    40981 cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaat ggagccacca cctcatttcg
    41041 cggggcgggt ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc tggcaccgtc
    41101 agtcattctc tctctgggga ttatttcaat taccgatggt cttggtggat tacgcgccgc
    41161 gcagcagtta atgacgcctg tgctaaaacc gttgttgggc attccaggga tctgctcgct
    41221 ggcgctgatc gctaacctgc aaaacactga tgccgccgcc ggtatgacca aagagctggc
    41281 gcaggaaggg gaaatgaccg aacgcgataa agtgattttc gccgcatatc aaaccagcgg
    41341 tagcgccatc atcaccaact acttctcttc cggcgtcgcg gtgttcgcct ttctgggcac
    41401 gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag ccaacatttt
    41461 gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg caaggagcgc aagcatgaca
    41521 actcaggtcc gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg tggctttact
    41581 atcgccacga ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat tcaggcgctg
    41641 aaaattaccg gcctgctcga ctgggtgggg catatctgtg aacccgtaat ggcgctgtgg
    41701 ggattacccg gtgaagcggc aaccgtcctt ttagccgcat taatgagtat gggcggcgca
    41761 gtcggtgttg ccgccagtct ggcgaccgcc ggcgcattaa ccggtcacga tgtcaccgtt
    41821 ttactgcccg cgatgtattt aataggcaac ccggtgcaaa atgtcggtcg ctgcctgggg
    41881 acagccgaag tgaatgccaa atattacccg catatcatta cggtctgcgt gattaacgca
    41941 ttgctgtcga tctgggttat gcagcttatt gtttaacaag gagttaccat gattgattat
    42001 accgcagccg gttttaccct gctgcaggga gcgcatttgt atgcgccgga agatcgggga
    42061 atttgcgatg tcctcgtcgc taacggcaaa attatcgccg ttgccagcaa tatcccttct
    42121 gacattgtac cggactgcac ggttgtcgat ctcagcgggc agatcctctg tcctggtttt
    42181 attgatcaac acgtccattt gattggtggt ggcggcgaag caggtcccac gacgcgcacg
    42241 ccggaagtgg cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt tggtctgctg
    42301 ggcaccgact ctatctctcg ccacccggaa tccctgctcg ccaagacccg tgcgctcaat
    42361 gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc ccgcaccatt
    42421 acgggttccg tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt gaaatgcgcc
    42481 atctctgatc accgttctgc cgcaccggac gtttatcacc tggccaatat ggcggcagaa
    42541 tcccgcgttg gcggtttgct cggcggtaaa cctggcgtca ccgtgttcca catgggcgac
    42601 agtaaaaagg cgttacagcc tatttatgac ctgctggaaa actgcgatgt gccgatcagc
    42661 aagctgctgc cgacccacgt taaccgcaac gtaccgttgt ttgagcaggc gctggagttc
    42721 gcgcgcaaag gcggcaccat cgatatcacc agcagcattg acgaaccggt cgcccctgcc
    42781 gaaggtattg cccgcgccgt tcaggcgggt attccgctgg cacgcgtcac cctcagctcc
    42841 gacggcaacg gtagccagcc gttcttcgat gacgaaggga atttaaccca tatcggtgtt
    42901 gcaggttttg aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta tgatttcagt
    42961 atcagcgatg ccctgcgccc gctcaccagt agcgtagccg gtttccttaa cctgaccggg
    43021 aaaggtgaaa ttctgccagg caatgatgct gacttgctgg tcatgacgcc agaactgcgc
    43081 attgagcagg tatacgctcg cggcaaactg atggtcaaag acggcaaagc ctgcgtgaaa
    43141 ggaacgtttg aaacggctta aacggttgtc agccgcccga atggacgtag aatgcccgca
    43201 agggcggctg acagagtaaa acgtaatgga tgactgtggt gcgattttgc ataatattga
    43261 aaccaaatgg ctttatgatt ttctgaccct ggaaaaatgc cgcaattttt cccaggcggc
    43321 agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc atccgtgcgc tggaacaggc
    43381 gattggtgtt gaattgttta accgccaggt aacgccgctg caactctcag aacaagggaa
    43441 aatcttccat tcgcagatcc gccatctgtt gcaacagtta gagagcaatc tggcagagct
    43501 gcgtggcggc agcgattacg cgcaacgtaa aatcaagata gccgctgcac actctctttc
    43561 cctcggtctg ttaccgtcca ttatcagcca gatgccgccg ctctttacct gggcaattga
    43621 agccattgat gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg actgtatttt
    43681 ttcctttcac gacgaagatt tgctggaagc gccgtttgac cacattcgct tatttgaatc
    43741 tcaattgttc cctgtctgcg ccagtgacga acacggagaa gcacttttta acctcgcgca
    43801 gccacacttt ccgttactga attacagccg caactcctac atggggcgat tgattaatcg
    43861 caccctgacg cgccacagtg agttaagttt cagcaccttt tttgtctctt cgatgagcga
    43921 gcttttaaag caggttgccc tcgacggctg tgggattgcc tggctgccgg agtacgccat
    43981 ccaacaagaa attcgcagcg ggaagctagt tgtactgaat cgggacgaac tggtgatccc
    44041 gattcaggcc tatgcatacc ggatgaatac ccgcatgaat cccgttgccg aacgcttctg
    44101 gcgtgaactg cgcgagctgg agattgtgct tagctgacat tctccagcgt cgcactgcgc
    44161 caaccgccat tatcccagac cagatcaaac gttagcggtt catcgtcgtg gtacagcatt
    44221 atctcaaggc tgatcgccag agtgtggcag tacgcttctt tcagattaac ctgcaacgcc
    44281 gcaatccgtg gctcatgaaa taacagtgca gaacgcagct gacggcaata ccagctctgg
    44341 agatgtttgt cgctcgggtt atgcacttgc cactcgggca tgcctgaaac aagtaatgac
    44401 ggtaatgccg aatgaggaga attaaaaaga gtgaggagat cgctgcatac cgatttacgc
    44461 agcaatgacg ttttattgcc tgaaatttca ggtaaaacag cctgaagtga ttggcgcatc
    44521 attttgcgca ctcctgacgt ttagcaacat atgcggcttg ccctgaacac cgggcaccac
    44581 tgtattgatg ctcattttcc atagccattc ccttttgttc attacatcca tgtcagtctg
    44641 agacaggcct gaatcctgtc agcaaagtat aaaaccttac ttgcctatga atatctactg
    44701 acttttcagg aaaaaagtca tgatttgata aaaatcatca aactcaaaaa aaaaccgcta
    44761 gtatattctg aatattccga atagttatgt agatgaaaat acttgtcacc gccgctactt
    44821 aattccggtg ggaaattatt tatttccgca taatcagcca tgtattat
//
LOCUS       DAZGCS010000029        44558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGCS010000029 DAZGCS010000000
VERSION     DAZGCS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.029"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..1783
                     /gene="adeD"
                     /locus_tag="ACHK01_001680"
     CDS             17..1783
                     /gene="adeD"
                     /locus_tag="ACHK01_001680"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO8096648.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1828..3219)
                     /gene="uhpT"
                     /locus_tag="ACHK01_001681"
     CDS             complement(1828..3219)
                     /gene="uhpT"
                     /locus_tag="ACHK01_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO8096649.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3357..4676)
                     /gene="uhpC"
                     /locus_tag="ACHK01_001682"
     CDS             complement(3357..4676)
                     /gene="uhpC"
                     /locus_tag="ACHK01_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8096650.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4686..6188)
                     /gene="uhpB"
                     /locus_tag="ACHK01_001683"
     CDS             complement(4686..6188)
                     /gene="uhpB"
                     /locus_tag="ACHK01_001683"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO8096651.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6188..6778)
                     /gene="uhpA"
                     /locus_tag="ACHK01_001684"
     CDS             complement(6188..6778)
                     /gene="uhpA"
                     /locus_tag="ACHK01_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO8096652.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6853..7143)
                     /gene="ilvN"
                     /locus_tag="ACHK01_001685"
     CDS             complement(6853..7143)
                     /gene="ilvN"
                     /locus_tag="ACHK01_001685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8096653.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7147..8835)
                     /gene="ilvB"
                     /locus_tag="ACHK01_001686"
     CDS             complement(7147..8835)
                     /gene="ilvB"
                     /locus_tag="ACHK01_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO8096654.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8941..9039)
                     /gene="ivbL"
                     /locus_tag="ACHK01_001687"
     CDS             complement(8941..9039)
                     /gene="ivbL"
                     /locus_tag="ACHK01_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO8096655.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9604..9693
                     /gene="tisB"
                     /locus_tag="ACHK01_001688"
     CDS             9604..9693
                     /gene="tisB"
                     /locus_tag="ACHK01_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO8096656.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9956..10030)
                     /gene="ysdE"
                     /locus_tag="ACHK01_001689"
     CDS             complement(9956..10030)
                     /gene="ysdE"
                     /locus_tag="ACHK01_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO8096657.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10112..11296
                     /gene="emrD"
                     /locus_tag="ACHK01_001690"
     CDS             10112..11296
                     /gene="emrD"
                     /locus_tag="ACHK01_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO8096658.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11304..11801)
                     /gene="yidF"
                     /locus_tag="ACHK01_001691"
     CDS             complement(11304..11801)
                     /gene="yidF"
                     /locus_tag="ACHK01_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8096659.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11798..12160)
                     /gene="yidG"
                     /locus_tag="ACHK01_001692"
     CDS             complement(11798..12160)
                     /gene="yidG"
                     /locus_tag="ACHK01_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO8096660.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12150..12497)
                     /gene="yidH"
                     /locus_tag="ACHK01_001693"
     CDS             complement(12150..12497)
                     /gene="yidH"
                     /locus_tag="ACHK01_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO8096661.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12606..13055
                     /gene="yidI"
                     /locus_tag="ACHK01_001694"
     CDS             12606..13055
                     /gene="yidI"
                     /locus_tag="ACHK01_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO8096662.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13102..14595)
                     /gene="yidJ"
                     /locus_tag="ACHK01_001695"
     CDS             complement(13102..14595)
                     /gene="yidJ"
                     /locus_tag="ACHK01_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8096663.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14592..16307)
                     /gene="yidK"
                     /locus_tag="ACHK01_001696"
     CDS             complement(14592..16307)
                     /gene="yidK"
                     /locus_tag="ACHK01_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO8096664.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRSTPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLSMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16474..17367
                     /gene="yidL"
                     /locus_tag="ACHK01_001697"
     CDS             16474..17367
                     /gene="yidL"
                     /locus_tag="ACHK01_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8096665.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17364..18178)
                     /locus_tag="ACHK01_001698"
                     /pseudo
     CDS             complement(17364..18178)
                     /locus_tag="ACHK01_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18178..19794)
                     /locus_tag="ACHK01_001699"
     CDS             complement(18178..19794)
                     /locus_tag="ACHK01_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8096666.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20090..20806
                     /gene="yidP"
                     /locus_tag="ACHK01_001700"
     CDS             20090..20806
                     /gene="yidP"
                     /locus_tag="ACHK01_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8096667.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20803..22464)
                     /gene="yidE"
                     /locus_tag="ACHK01_001701"
     CDS             complement(20803..22464)
                     /gene="yidE"
                     /locus_tag="ACHK01_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO8096668.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22661..23089)
                     /gene="ibpB"
                     /locus_tag="ACHK01_001702"
     CDS             complement(22661..23089)
                     /gene="ibpB"
                     /locus_tag="ACHK01_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO8096669.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23201..23614)
                     /gene="ibpA"
                     /locus_tag="ACHK01_001703"
     CDS             complement(23201..23614)
                     /gene="ibpA"
                     /locus_tag="ACHK01_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO8096670.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23608..23680)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23920..24252
                     /gene="yidQ"
                     /locus_tag="ACHK01_001704"
     CDS             23920..24252
                     /gene="yidQ"
                     /locus_tag="ACHK01_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8096671.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24254..25468)
                     /gene="yidR"
                     /locus_tag="ACHK01_001705"
     CDS             complement(24254..25468)
                     /gene="yidR"
                     /locus_tag="ACHK01_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO8096672.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25569..26633
                     /gene="cbrA"
                     /locus_tag="ACHK01_001706"
     CDS             25569..26633
                     /gene="cbrA"
                     /locus_tag="ACHK01_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO8096673.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26630..27955)
                     /gene="dgoT"
                     /locus_tag="ACHK01_001707"
     CDS             complement(26630..27955)
                     /gene="dgoT"
                     /locus_tag="ACHK01_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096674.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVTYFIAIFGW
                     SVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQ
                     FVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDY
                     IRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPN
                     YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLL
                     ISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAG
                     GLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28030..29178)
                     /gene="dgoD"
                     /locus_tag="ACHK01_001708"
     CDS             complement(28030..29178)
                     /gene="dgoD"
                     /locus_tag="ACHK01_001708"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO8096675.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29175..29792)
                     /gene="dgoA"
                     /locus_tag="ACHK01_001709"
     CDS             complement(29175..29792)
                     /gene="dgoA"
                     /locus_tag="ACHK01_001709"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO8096676.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29776..30654)
                     /gene="dgoK"
                     /locus_tag="ACHK01_001710"
     CDS             complement(29776..30654)
                     /gene="dgoK"
                     /locus_tag="ACHK01_001710"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO8096677.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30651..31340)
                     /gene="dgoR"
                     /locus_tag="ACHK01_001711"
     CDS             complement(30651..31340)
                     /gene="dgoR"
                     /locus_tag="ACHK01_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO8096678.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31618..32274
                     /gene="yidX"
                     /locus_tag="ACHK01_001712"
     CDS             31618..32274
                     /gene="yidX"
                     /locus_tag="ACHK01_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO8096679.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32320..33132)
                     /gene="yidA"
                     /locus_tag="ACHK01_001713"
     CDS             complement(32320..33132)
                     /gene="yidA"
                     /locus_tag="ACHK01_001713"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO8096680.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33247..33645)
                     /gene="yidB"
                     /locus_tag="ACHK01_001714"
     CDS             complement(33247..33645)
                     /gene="yidB"
                     /locus_tag="ACHK01_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO8096681.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33885..36299)
                     /gene="gyrB"
                     /locus_tag="ACHK01_001715"
     CDS             complement(33885..36299)
                     /gene="gyrB"
                     /locus_tag="ACHK01_001715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO8096682.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36328..37401)
                     /gene="recF"
                     /locus_tag="ACHK01_001716"
     CDS             complement(36328..37401)
                     /gene="recF"
                     /locus_tag="ACHK01_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO8096683.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAQYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37401..38501)
                     /gene="dnaN"
                     /locus_tag="ACHK01_001717"
     CDS             complement(37401..38501)
                     /gene="dnaN"
                     /locus_tag="ACHK01_001717"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO8096684.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38506..39909)
                     /gene="dnaA"
                     /locus_tag="ACHK01_001718"
     CDS             complement(38506..39909)
                     /gene="dnaA"
                     /locus_tag="ACHK01_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO8096685.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40206..40286
                     /locus_tag="ACHK01_001719"
     CDS             40206..40286
                     /locus_tag="ACHK01_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096686.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40516..40656
                     /gene="rpmH"
                     /locus_tag="ACHK01_001720"
     CDS             40516..40656
                     /gene="rpmH"
                     /locus_tag="ACHK01_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO8096687.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40673..41032
                     /gene="rnpA"
                     /locus_tag="ACHK01_001721"
     CDS             40673..41032
                     /gene="rnpA"
                     /locus_tag="ACHK01_001721"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO8096688.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40996..41253
                     /gene="yidD"
                     /locus_tag="ACHK01_001722"
     CDS             40996..41253
                     /gene="yidD"
                     /locus_tag="ACHK01_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO8096689.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41256..42902
                     /gene="yidC"
                     /locus_tag="ACHK01_001723"
     CDS             41256..42902
                     /gene="yidC"
                     /locus_tag="ACHK01_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO8096690.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43008..44372
                     /gene="mnmE"
                     /locus_tag="ACHK01_001724"
     CDS             43008..44372
                     /gene="mnmE"
                     /locus_tag="ACHK01_001724"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO8096691.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10763 a        12092 c        11444 g        10259 t
ORIGIN      
        1 tcaaaatgag aaaaatatga ataattctat taaccataaa tttcatcaca ttagccgggc
       61 tgaataccag gaattgttag ccgtttcccg tggcgacgct gttgccgatt atattattga
      121 taatgtctct attctcgacc tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa
      181 aggacgttac attgccggtg ttggcgcaga atacgctgat gctccggctt tgcagcggat
      241 tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat gctcacctgc atattgaatc
      301 cagcatgatg acgccggtca cttttgaaac cgctaccctg ccgcgcggcc tgacgaccgt
      361 tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa gccggattcg cctggtttgc
      421 ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtacccgc
      481 cctggaaggc tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg
      541 ggaccatccg caggttaccg gccttgcaga aatgatggac taccctggcg taattagcgg
      601 gcagaatgcg ctgctcgata aactggatgc atttcgccac ctgacgctgg acggtcactg
      661 cccgggtttg ggtggtaaag aacttaacgc ctatattact gcgggtattg aaaactgcca
      721 cgaaagttat cagctggaag aaggacgccg gaaattacaa ctcggcatgt cgttgatgat
      781 ccgcgaaggg tccgctgccc gcaatctcaa cgcgctggca ccgttgatca acgaatttaa
      841 cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg tgggagatcg cccatgaagg
      901 acacatcgat gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc
      961 atatcgcgtc gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact
     1021 ggcacccggc aagcaggccg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca
     1081 gcaggtactg gtgaaaggcg agccgattga tgcgcaaacc ttacaggcgg aagagtcggc
     1141 gagactggca caatccgctc cgccatatgg caacaccatt gcccgccagc cagtttccgc
     1201 cagcgacttt gccctgcaat ttacgcccgg aaaacgctat cgggtcattg acgtcatcca
     1261 taacgaattg attacgcact cccactccag cgtctacagc gaaaatggtt ttgatcgcga
     1321 tgatgtgagc tttattgccg tacttgagcg ttacgggcaa cggctggctc cggcttgtgg
     1381 tttgcttggc ggctttggac tgaatgaagg tgcgctggct gcgacggtca gccatgacag
     1441 ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg gcgctggcgg tcaatcaggt
     1501 gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc caggtccaaa gtcatctgcc
     1561 gttgcccatt gccggcctga tgagcaccga cacggcgcag ttgctggcgg aacaaattga
     1621 cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt ttattcagat
     1681 ggcgtttctt tctctgccag tgatccccgc gctaaaacta accagtcagg ggctatttga
     1741 tggcgagaag tttgccttca ctacgctgga agtcacggaa taataaaaaa gcccggcgtc
     1801 atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc
     1861 gcggcggatt ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga tacaaccaat
     1921 cgcggcgata tcaagtgcgg cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt
     1981 cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa
     2041 ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagcagc
     2101 cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag
     2161 atagatatat tcgttgctgg catgttgata cacaccgagc gtggcgataa tcagcgccag
     2221 cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag
     2281 cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt
     2341 agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac caatacgtac
     2401 cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata
     2461 ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt cctcttcgct
     2521 gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg gggagtcgct
     2581 gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga agataaacat
     2641 gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac ctgctgcgcc
     2701 tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc gtttacgacg
     2761 cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct ggaaaaagcc
     2821 gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc ccatactggc
     2881 actgaagccc agcatacaaa tggcagacag gatcagcatg aacggcagga attgtttggt
     2941 gtttttgccg tcggcgtagt aggaaaccag cgttttaccc acgccataag tgatggagaa
     3001 acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaga tcatgtcgtt
     3061 ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga taaagaccac
     3121 caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc
     3181 cagggtcggc ttgcgaacct ggtttaagaa agccagcatg ggttactcct gaaatgaata
     3241 cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat agcgtccagg
     3301 caaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga gatgcatcac
     3361 gcttcgcgcg gtgcctgggc gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg
     3421 gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagtac tttcgccagc
     3481 ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc
     3541 cctgccgcct ctttgtggga acactctgcc gccgccatgc cgattaacat ctgcgggcca
     3601 aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc
     3661 atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc
     3721 ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc accgataaat
     3781 ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac gcccagtgtc
     3841 tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata gaccagcaca
     3901 tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt gaggatctct
     3961 ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc gtgtcgccat
     4021 tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca gcagagaaaa
     4081 atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca gccgtaatgc
     4141 agcgcagccg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc
     4201 cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa cagacgcgca
     4261 cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac ggcaaacgcc
     4321 catagcgacg tcgagaagcc aaacagaatg ttgatgatgc ccgtggcgat aagccctatc
     4381 cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg
     4441 ccgtaggtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct gagcacgccg
     4501 ttagcaagga tttctggtac ggcggcgtta aaacttttac gcgtgaagta aaacagcgcg
     4561 taacccagcc agatggtcag cagaatatgc cgacgccagt agcgatagcg ggcatcaatt
     4621 tcatatttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc
     4681 aaaccttaga catagcgttg aggtagagaa acgctgacac gcgtgccgtg cagacaggaa
     4741 atgtgtaatg tgccacccag cgccgttacg cgctcgcgca ttccggtgag gccaaaacct
     4801 tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caataaccag catcaaccgt
     4861 tcatcctgct gccagccttg cagcgtgacc gcgctggcat cagcatgttt cacaatgttg
     4921 ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcact taatgctgat
     4981 tcatcgattc gccattcgag atggctgaca ataccgcgcc cttccagctc catttcccgc
     5041 atcagtgagc ggatggcctg ctccagggtg agatcatcca actggcgcgg acgtaaccga
     5101 cccaacaaac ggcgcaccgc gtcgtaaacg cccagcgata gttgttcgat gagctgcccg
     5161 ctctgcttca cgctggcgtt atctgccgcc agccgctgaa caatgcccgc ctgagtacga
     5221 atagcagtga tggtctgacc gatatcatca tgcagctcac gcgccacatc acgacgcacg
     5281 ctctcttcgg tttccagcaa ccgttcagcc agatgctgat tgcgcgccag ttccttttgc
     5341 agcgactggt taagttcacg caaccgctgg atgccagcgc ccagtaacaa ccctgtcaga
     5401 ctttgcacca gcaacgagag caataaatcc accggatgat cgcgccaggt ttgactggcg
     5461 atcagcgcga tggcgttcat caacgtcgcg atcagcgccc cttgccaacc gtagtgccag
     5521 gccagcgcga taatcggcag cgccaggcag aatggcgtaa agcgcgacag ttcgtccggc
     5581 aatcccaact ggagccagag actaataaca aacaacagca agtaccagac cagatgccgc
     5641 ccgcgccagt tgatcggttg agaaaccagt gacgggccga gcggcagcca ggtgttattg
     5701 gcgagatagt gccagaacac cagacatatc ggggccagcg tcaggccgcc agttaaagtc
     5761 agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga ctgcaacaac
     5821 gccgccgccg ttaacgccgc cccctgtaac agcaaggtgc gccagtcacg ctgatggcga
     5881 tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac taccgatcat caataacgga
     5941 aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccattc cgcacccagc
     6001 aatacgggcc agtatccgcg cgggcattgc agcattagcc ctagccgcag accaaacgga
     6061 aataacagca ccgccatatc agggcgctca accaggtgca ggctgatact ccacaggcaa
     6121 aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg ggagaacaac
     6181 gtcttcatca ccagccatca aacatgcggc gcgccagttc tacgtcgtta ctgacgccca
     6241 gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag cccagttcgg
     6301 cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg cgttcacgtt
     6361 tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc gtcagataac
     6421 agccgcccgt agcaaccgta tgcaccgccg caatcagttc gtcagggcta cagcgtttgg
     6481 agagaaagcc gcgcgccccc gcgttaagcg cctgctcaac cagcgccgga ctgtcatgaa
     6541 cagagagcat tattgtcgcc atacctttcg gcagctggct tagcagttcc agaccggaga
     6601 tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc agccccgcca
     6661 gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc agccccagca
     6721 gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg gtgatcatgg
     6781 tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg atgttgttca
     6841 agccttgagc ggttactgaa aaaacaccgc aatcttgtta aacatagtcg gatcggactg
     6901 attacgctgc actttcacca catcttccag cttatcgatt tggcttatca tctgctccag
     6961 acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct gaatcggcag
     7021 acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa cgtgggtcat
     7081 tacgcccgga tggttgcgaa cggtgagctc cagaatcacg ttgtcatgag ttgtgttttg
     7141 catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca tcgggtaaac
     7201 tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat tgatgatttc
     7261 ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga ggccgaatcc
     7321 ggcggcaatc tgcataaagt tgatttttcc cggataggtg gcggcaaaaa cgccttgctc
     7381 gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca tcagaatgat
     7441 tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca tcatcaggct
     7501 gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg cgccaatcgc
     7561 cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact ggcgtgggcg
     7621 attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg tggtgataat
     7681 cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc ttaatggatc
     7741 gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca actggtgcca
     7801 ctctgcacgc ggttgcgctt ccacctgcgg gatcaattgc gccagcacgt catcaacatc
     7861 cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat cgatatcgac
     7921 atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc ggtcatcaaa
     7981 acgcgcaccg agcactatca acaaatccgc ctcctgcaaa atatagttgg tgctgcgcac
     8041 gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcaaca tgcccagcgc
     8101 cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca cccgtgcggg
     8161 cgcattgatc acaccgccgc ccagataaag caccgggcgt ttggcagcgt taatcatcgc
     8221 cgctgcgtca cgaatgcttt cttcgctaaa ggcgggggcg gcggcttttt ctgccatagc
     8281 gggctgtgtt tcaatctcaa aaactgccgt ttgcacatcc ttaggaatgt ctatccacac
     8341 cgggcctggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct gcgggagttc
     8401 ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc
     8461 cacttcctgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccgg tgatgcaaat
     8521 cagcgggatg gagtccagcc gcgcatcggc aatggcggtc accaggttag tcgcacccgg
     8581 tccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccatcc cctgagcgat
     8641 aaatcccgcg ccctgttcat gacgggccag aatatggcgg atttgcgtgc tttggcttaa
     8701 ggcatcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct taatgccctg
     8761 ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg tcgatgttgt
     8821 gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg aggtgcttaa
     8881 acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc
     8941 ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg cggatagcgc
     9001 agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg ttggttttgt
     9061 gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa tttttcagca
     9121 agggaaaatt gaggggctga tcacgttttg tactgaattg cagataacaa aaaaccccgc
     9181 cggagcgagg tttcatcagc cgcctgcggc tggtaaccgc aaagcacact gtattatgtc
     9241 aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta
     9301 tttagggcat agttgagtat cgatcacagt ttgcgttttg tccaaatatt actgtttatt
     9361 tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg ccgcatcgtt
     9421 acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta accccgcgtg
     9481 agcacactgt gttatgtcaa caagcacaac gtttctcctt aagataccgc gtgcacaaca
     9541 gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc acaggagacg
     9601 cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc agcactgcaa
     9661 ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca ccaaagggga
     9721 gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagttggggg cttcttctac
     9781 cagcagaatg tttgcacctg cgctgcctgt gcccctcacc ctagccctct ccccaaaggg
     9841 gcgaggggac cgtcccgtgc aatgtttagc tcattttctg aaagggaacg gtggtttccc
     9901 cctcgcccct ttggggagag ggttacagtt cgcacgtaat ccctcccggc aacatttatt
     9961 gccgcttttg tttacatatt ctgccgctaa acaattctcc attcctggcg tatatctggc
    10021 taacattcat caatgtgata gattcctctc ccgcatttat gggaatgcgt agtgacttat
    10081 tctaattatt tttataaaag catccgtgat aatgaaaagg caaagaaacg tcaatttgtt
    10141 attgatgttg gtattactcg tggccgtcgg tcagatggcg caaaccattt atattccagc
    10201 tattgccgat atggcgcgcg atctcaacgt ccgtgaaggg gcggtgcaga gcgtaatggg
    10261 cgcttatctg ctgacttacg gtgtctcaca gctgttttat ggcccgattt ccgaccgcgt
    10321 gggccgccga ccggtgatcc tcgtcggaat gtccattttt atgctggcaa cgctggtcgc
    10381 ggtcacgacc tccagtttga cggtgttgat tgccgccagc gcgatgcagg ggatgggcac
    10441 cggcgttggc ggcgtaatgg cgcgtacttt gccgcgagat ttatatgaac ggacacagtt
    10501 gcgccatgct aacagcctgt taaacatggg gattctcgtc agtccgttgc tcgcaccgct
    10561 aatcggcggt ctgctggata cgatgtggaa ctggcgcgcc tgttatctct ttttgttggt
    10621 tctttgtgct ggtgtgacct tcagtatggc ccgctggatg ccggaaacgc gtccggtcga
    10681 tgcaccgcgc acgcgcctgc ttaccagtta taaaacgctt ttcggtaaca gcggttttaa
    10741 ctgttatttg ctgatgctga ttggcggtct ggccgggatt gccgcctttg aagcctgctc
    10801 cggcgtgctg atgggcgcgg tgttagggct gagcagtatg acggtcagta ttttgtttat
    10861 tctgccgatt ccggcagcgt tttttggcgc atggtttgcc ggacgtccca ataaacgctt
    10921 ctccacgtta atgtggcagt cggttatctg ctgcctgctg gctggcttgc tgatgtggat
    10981 ccccgactgg tttggcgtga tgaatgtctg gacgctgctc gttcccgccg cgctgttctt
    11041 tttcggtgcc gggatgctgt ttccgctggc gaccagcggc gcgatggagc cgttcccctt
    11101 cctggcgggc acggctggcg cgctggtcgg cggtctgcaa aacattggtt ccggcgtgct
    11161 ggcgtcgctc tctgcgatgt tgccgcaaac cggtcagggc agcctggggt tgttgatgac
    11221 cttaatggga ttgttgatcg tgctgtgctg gctgccgctg gcgacgcgga tgtcgcatca
    11281 ggggcagccc gtttaagcgc acgtcaccgc agcatcgtca tcagctccat gggagaacga
    11341 tgctgcttta tcagatcacg catcacccgc atatgcggtg cggagtaaga ataaaacgcc
    11401 tgatagcccg cacaaagcac gctgtctggc gtctcctggc gagcgtgcgg gcacaggcga
    11461 tgccagtcgc acgcctggca gtcacggcgc ggttgcgggt tttcgctgcc acaccatttt
    11521 tgtaaggtgg cgtcaaaaag ctcaatcgag atacgactga tatctgcccg cacccaacac
    11581 tcgaacaggg caattaaaaa tttcccccac agctctgctg tcaccgaagc ggccgtcaga
    11641 tcgccccggt catcaaattc aagataggga atgagctgta cggagcggat gccccgctgt
    11701 ttgaaattgt gataaaaatc ggcaggttgc agagcatcct tgtactctac aaccagttta
    11761 tgcctgtcct cttcagcatc aatgacctga cttcctgtca tgcgactctc tcaataaata
    11821 cgataagttg atgtatatgc gttacagcaa acagtattgc gagagataac accgcgaggg
    11881 agatcaaaaa tttgtcacgg actacgtgaa attgggaaaa atcgctattc gtgacatcca
    11941 ttaaattgcg attacgggtg tagtgccaga ggatcagcgc cacgatggcg agaatgccaa
    12001 tcgaaatcca gaataacatg cccgcctggt gccagttgtg tttgatagcc aacgccatca
    12061 acgcgccgta gcccagcatg gtacgaaacc acgccagtga tgtgcgctcc ggctgcagtc
    12121 ctgggtcggc aatgcgtctg gctttgcggc tatccggcat acaacaccag tcccataacg
    12181 atcaccgcca cgaccatcaa tattaagcta atgattaata agctgttggt gtagggcagg
    12241 tcctctttca gacgcatcgc tttttcattg cgtaaccagc gcaggtagcc atacatcgct
    12301 aacccaccgg aaaatagaca caacaacaac gccagcagtt cgcgaatgac gggcgtggcg
    12361 aaatctggcg caagttggtc aagcccaacc cccgccgcca gaaatcctag tgcggtacgg
    12421 atccacgcca gaaaagtacg ctcattagcc agcgagaagc ggtaatcggg cgcttctccg
    12481 aggcgggaaa ttttcattgt tgctcctttg caatttcact aacgctctat ctttagcggt
    12541 tttttttaag aatttcgtcc gcccgtttcc tgatatgctt ttgccatccc ctacaaggag
    12601 gtcagatggg tatcatagcg caaaataaaa tcagttcatt gggcatgctg ttcggtgcca
    12661 tcgctttaat gatggggatt atccactttt cattcggccc tttttccgca ccaccgccaa
    12721 cgcttgaaag catcgtggcg gacaaaacgg ctgaaattaa aagaggtctt ctcgccggaa
    12781 taaaaggcga aaaaattact accgtggaga aaaaagagga cgtagatgtc gataaaatcc
    12841 tcgatcaaag cggtatcgca ctggccattg ctgcgctact ttgcgcattt attggcggta
    12901 tgcgtaagga aaatcgctgg ggcattcgcg gcgcgctggt ttttggcggc gggacgctgg
    12961 ctttccatac cttgcttttt ggtatcggca tcgtgtgcag cattttgctg atattcctta
    13021 tcttttcctt cctgactggc ggttcactgg tttaagcagc ctgaataatg ctcgccgggt
    13081 gttcatcagc gggcattatc gtcagaactt ctgttttttc tcttccactt tcactccttg
    13141 cgtcggtagg ccggtgtcat agtcacgtac caccggcgaa tagccatctt gtgggcgtgg
    13201 acgaaatgcc cccatccagc gcggcagtgc atctttacgc cacggacgca ggctccattg
    13261 gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt catgcatttt
    13321 gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat ttgggtcatt
    13381 acggcgatcg taaagttcat cactggtgaa gaggttgagt accagtttaa agtcatccgt
    13441 cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct cgtagcggtt
    13501 aaattccacc attacaccgc gtggctcttt cacggcaagg atattttccc ccggcagaat
    13561 ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcga tatgactgac
    13621 tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg gaatacgggt
    13681 gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca tcatttcgcc
    13741 gtgatcggaa gtataaatga cccacgtatt ttcacgttgc tctggcgtta aggcattgat
    13801 gacccgtccg atttggtcat caacaaagtc attacaggca aaatagagcg gatggtgata
    13861 aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt gatgttccgg
    13921 tttgttcgcc aggtcatcct gagctttctc gcccagatcg tagtaaaaat cagcgtattt
    13981 ctctaaatac tccaccggac aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat
    14041 caggaagggt tcctcggcgc gcgcgggctg ttgcagaaaa tctaccgccc gattgctgat
    14101 gcggtgcgcc caggtgaagg tttcgtcgat atggttcgcc tgtaaatcct caacgctgtt
    14161 taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaaggt agttcgcccc
    14221 atcgaaccag taatcagcgt cccactccgg cggacactcg ccagtgccga aatagtcatg
    14281 accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct taaagtagcg
    14341 ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc cggactggtt
    14401 agcgtagata ccggtaaata gtccggcgcg tgcaggcgta caaaccggtg aacaggtgta
    14461 ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg tattcagcgg
    14521 tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcatga tgaacagaaa
    14581 attggggcgt ttcataggct atccttcgcg tcgggagtaa aggttacggc tgggtcgtga
    14641 cgatgccgcc agctgtcaaa aatcaggtag acaatcacta cggcggcaat gaaataactg
    14701 atcatcgata accagcgcgt accgtagccg ccgaattcag ccagcccggc atagacgcca
    14761 atcatcgcga acaggatgcc aattgacgcg atcttgacgt ttctccacgg tttcatgtcc
    14821 accgcaaacg catctttgaa ggtgaacggc gtggagcgcg gtttgataaa accgatcacc
    14881 agcatcacga ccacgttgat gcagaacgta caggccagca catagaggaa atggaagtcg
    14941 aacttcacca gatagttgat ggtgatgtag ctgattatgc caatccccat cgccactttt
    15001 gccgccagcg ccgggatgcg cgggaagaaa aagcccataa tgatgatggt aaccagcggc
    15061 acgttgtaaa tgccgttgag ctgtttcatc cagctataca gcccctgcgg cgcgttggcg
    15121 atccacggcg cgaccagcac cgaaacgata gcgataaaga aaccaaattt gcgcccgacg
    15181 gtgaccagct gctgcggctc ggcattctgg ttaatgatgc gacggtaaat acccatactg
    15241 aataacgtac tggcgctatt cagaaagccg ttgaaggtac tgatcaccgc accaaataac
    15301 accgcgccga agaaacccac cattggcact ggcagaacgt tattgaccag cgtcgggtag
    15361 gccatgtcgg ctttcggtaa atcctgatac agatgaaacg caatcaaccc tggcagcacc
    15421 agtaccagcg ggtcgagcat tttcagcacc gccgttaaca gcgccccctt ttgcccttcc
    15481 gccaggcttt ttgacgccag cgtgcgttgc acgatgccct gatttgtaca ccagtaaaag
    15541 gtgttcacca gaatcaaacc ggtaaatgcc gcgccaatcg gcaagggatc ggtcgggcca
    15601 ccgattgagt ttaatttctc ggcgtgaacg gtggtgagtt gctcaatgcc ctgcataaag
    15661 ctgcccttgc ccatcgcgat caggccaaat accggcacca tcaacccgcc gataaccagc
    15721 ccaataccgt tgatggagtc cgccactgcc attgcgcgca gtccgccgat caccgcatac
    15781 aaaattcccg ccagaccaag caaaattacc agtagccaga tagccgcacc gtgagaaatc
    15841 tgtagcgatt ccccgacgtg aaacaggctg ttgagcgcca acgcgccgga gtagagaaca
    15901 atcggcagaa agcagacgcc ggtggcaatg aggaagcaga agtcgatgat aatacgcgtc
    15961 gttttatcat aacgttcctc cagaaaatcg gggatggtgg caatgccgcg cttcagatag
    16021 cgcggtaaaa agattagcgc gaggaagatc agcgtcaccg ctgaagtcac ttcccagccc
    16081 atcaccgaca tgccgctttt gtaggcctgc ccggaaaggc cgaccagttg ttccgtggaa
    16141 aggttggtta gcattaacga agcggcaata accggcgctt ttagtgaacg tccggcaaga
    16201 aaatagcctt gttgcgatcc ggtatctatt ttgcggacct tccaccaggt gatcaccgcc
    16261 accagcagcg taaaaccgac aaaactcaag atttgtaacg aattcatctc atagccctta
    16321 tttatgttta tgtcccggac gcatgaatat agggagaggt aaaatgcgtc gggtcaggca
    16381 gtcagattct taaaatacaa acgtcgtatc cctgaacgga ttgttttctg ttaagttcag
    16441 gttgtgagca taattcgccg gagggatatt aaaatgaatg gaaaattgca aagttcagat
    16501 gtaaaaaacg aaactccgta caatattccc ttattgatta acgaaaatgt gatctccagc
    16561 ggaatttctc tgatctcgct ctggcatacc tacgccgacg agcattaccg ggtgatctgg
    16621 ccgcgggaca agaagaaacc gcttatcgcc aactcatggg ttgcggtgta taccgtacaa
    16681 ggatgtggga aaattctttt aaagaatggc gaacaaataa cactgcatgg caactgtatt
    16741 atattcttaa agccaatgga tattcactct tatcactgtg aaggtttagt ctgggaacag
    16801 tactggatgg aatttacccc caccagtatg atggatattc ccgttggtca gcaaagcgtt
    16861 atttataatg gcgaaattta taatcaggaa ctcaccgaag ttgctgagtt aataacttca
    16921 ccagaagcaa taaaaaataa tctggcagtc gcttttctga cgaaaattat ttatcagtgg
    16981 atttgtctta tgtacgcaga tggtaaaaaa gatccacaac ggcggcaaat tgaaaaatta
    17041 attgccactt tacatgccag tctgcaacaa cgctggagcg tagctgatat ggctgccacg
    17101 atcccctgta gcgaagcctg gttgcgtcgt ctgtttttac gctataccgg caagacgccg
    17161 aaagaatatt acctcgatgc gcgtctggat ctggcgctat cgctattaaa gcaacaagga
    17221 aactcggttg gcgaagtcgc tgatacgctc aacttcttcg actcctttca tttcagcaaa
    17281 gcctttaaac ataaatttgg ttatgcgccg tcagccgtgc taaagaatac ggaccagcac
    17341 ccaacggatg ccagtccaca caattaacgt aactctggcc agtacgcttt gttggcctcc
    17401 agcaactcat ccagaatagc tttggcgacc gaggcgctcg gtaccgtttt cgacaacgta
    17461 atcgcctacc acaggtgctg atatgaacgc tgttcccagg cgtccacgac cagtttttcc
    17521 accgccactt gctgactcat taaccctttc tgaaactgcg ggatatcgcc gaccaccagt
    17581 ggttcaggcc gtagtagcgc acgcgcatct gtttgcgatc ttgcagccca acaatttgcg
    17641 ccatccggct ttcaataccg attggcatgt cacagatgtt gaggattttc gcattcgggc
    17701 gcagacgacg cgtggcttct gcgacaattg ctgccgggtt ggagtagttg agcatccagg
    17761 cgtttggtga atatttttcc atataatcca ccagttccag gacgccgcca atggaacgca
    17821 tgccgtacgc tattccgccg ggtccgcaag tttcctgacc aacaacaccg tggcgcagtg
    17881 ggattttttc atccagttcg cgcatcgggt acttgccgac gcggatgtgc gccatgacaa
    17941 aatcaacgtc gctgaatgcc acttcaggat cggtggtgta actaaacgca atgtccggcg
    18001 ctttctcttt aaggatgact ttacaggctt cggcaatcac ttcctgccgc gcaccatcgt
    18061 tatcataaaa tttcagtgca cgaagcggga aacggtcctg attcgccagg agcatcaaca
    18121 cgatgcctgg cgtaaaggtg cttccaccgc ctgcgacaac cactgagaat ttggtcatta
    18181 tactgcctcc gtaatggcaa cattttctgc tgattgatga gaattaatta agctatcgag
    18241 ctgttcacgc agctgggata catgcagacc aatgatcacc tgaatggcat cgccactacg
    18301 gaagacgccg tgcgctccca gctttttaaa gacttcgtca tccagcgttt gtgacatgtc
    18361 atgcagtgca atacgtaaac gcgtcgcgca attgttaatg ctggagatat tgccgacccc
    18421 gcccagggct tgcaggatac cggcagcctg atccagctct ttttttggct ctgccgcggt
    18481 ggtttggcct cgcgaggctt tgtattcggc ttttgagtag agtttcactt ccgcatcttc
    18541 acgtcccggc gtgcacatgt tgaactgcag aatcagtgtg cggaaaacca cgaagtacag
    18601 cagggtaaag cacaacccaa tggcgatttg ggtcagcatc atatccgcgt ggttgctgaa
    18661 catcgggatc cagttttgcg gtaaaacctg gtcaatcaga cctccgccca tgttgcccac
    18721 cacaccaaag agatacatta cggtcgacat tgaggccgcc agcacggcgt gtaccgcaaa
    18781 cagcaacggt gaaatgaaca ggaaggtaaa ttccagcggt tcggtaattc ccaccagcat
    18841 ggcggttaag gttgcgggaa tcagcaagcc cgccactttt acccgatttt ccggtgcggc
    18901 agtgaagtac atcgctaaag aaatgcccac ggcaccaaag atttttgagt taccgtgcag
    18961 ggcaaaaccg ccttccggga acaacgattt cagcggctcg gcgctcaaac tgaactcttg
    19021 cagatgctgc gcccagtaca tctgaatgcc gccttcaaca gctgccggac caaagataaa
    19081 ctgtccgtag atgaagtggt gtaaaccggt tgggatcaga atacgttcga ggaaggtgta
    19141 aacccagacc ccaagtgcac ccgccgaacg caggaacgct tgcagagatt caatccccat
    19201 ttgtactttt ggccagccga gcaacgtcag ccaggcacag gggatcatca ccaggaaggc
    19261 gataatcacc acataagacg tcccctggaa aatgccgaga aaaaccggca gttttttatc
    19321 gaacagacgg ttatgcagcg ccgtcacaat gccggaaatg ataattgcgc cgataatgct
    19381 ggtatcgagg gttttaatcc cagccatcat tgtcagaccg ctacctgcca ccgcgtcctg
    19441 agtgaaatcg acgccgaagt agcttcccca ggtcattccc atcgcattga tgaaatagtt
    19501 ccaggtcagg aaactcacca tcaccgccag acaagcacgt ccctgcgctt gcttagcaag
    19561 gccaatgggt aaaccgacag caaaaatcag cggcatatta cggaataccg tccaaccgcc
    19621 ctcttcaata atgtgtacga tttgcgcgaa taaactgttc ggatcggtca gtgattcccc
    19681 gacaaacatc gggttttgca gcaagatggc aagacccacc acaatcccgg cgaagggaaa
    19741 cagcagcact ggcgtgaaca tcgcgccgcc aaagcgttga atttgactga gcatttccac
    19801 atcctctttt ctcaattctg taagtaggaa gcctgattgg ttatgcggca ggcctggagc
    19861 gaggatagga agatgcgatc ggcagaacat gacgacccaa tgggattcgt gatcgctttc
    19921 atgcttttcg ttgggcggta gagatctacg ttttcgcgaa aaagtagaca tctgtgagca
    19981 ctggtacaca gcatcgaaca catctttaaa aaaaagatgt tttttcaatc gattaagcag
    20041 aacttgtggg cgcattaccc gggcttgcag gcaaaaaaga gatctagaga tgatctacaa
    20101 aagcattgcg gagcggttaa gaattcgact taattacgcg gatttcacgc taaacagcct
    20161 tcttcccggt gaaaaaaagt tggcggaaga atttgcagta tcccggatga ccatccgtaa
    20221 agcgattgac ctgctggtag cgtgggggct ggtggtccgc cgccacggca gcggcactta
    20281 cctggtgcgc aaagatgtgc tgcatcaaac cgccagcctg accggactgg tggaggtgtt
    20341 aaaacggcag ggaaaaacgg tcaccagcca ggtgctgatt tttgaaatca tgcctgcgcc
    20401 tccggccatt gccagccagt tacggattca aatcaacgag cagatctact tctcccgtcg
    20461 cgttcgtttt gtggaaggga aaccgctgat gctggaagac agctatatgc cggttaaact
    20521 gttccgtaat ctttcgctgc aacatctgga agggtcgaag tttgaatata ttggacaaga
    20581 gtgcgggatt ttgattggcg gtaattatga aagcctgacg ccggtgctcg ccgatagact
    20641 gctggcgcgg caaatgaagg tagcggaaca cacgccactg ctgcggatca cctcgttgtc
    20701 atatagcgag agcggggagt ttttgaatta ttcagtgatg ttcagaaatg ccagcgaata
    20761 ccaggtggag taccatttac ggcgactcca cccggaaaag agttaaccga tactccagaa
    20821 gagcaccgcc agtaattggg gggtgataat gcgcagaaac atcactaacg gatagacagt
    20881 ggcgtaagag agcgccgccg caccactggt tggatgaaga ttattagcaa acgccagcgc
    20941 cggaggatcg gtcatggaac ctgccagcat cccgcacatg gtcaggtaat tcattttggc
    21001 taacatccgc gccagaatgc caacagtaat cagcggaacg gcggtgatca gggcaccata
    21061 accaatccag cttagccctt cgccattgac cagggtattc acaaaatccc caccagattt
    21121 cagaccaacg accgagagga acagcacgat ccccagctcc cgcagcgcga ggttggcact
    21181 tggcggcata aaccagtaca gcttgccgat actgccgata cgcccgagga tcaacgccat
    21241 aatcagcgga ccgcccgcca gccccagttt caacgcggcc gggaacccag gcacaaagac
    21301 gggaatagaa cctaacaata cgccaagccc gatgccaata aacactggca gcatctgaac
    21361 ctgttgcagt ttttgctgcg cattccccag cacattggca acggcatcaa ttgcggacgg
    21421 acgccccacc agattgagga tatcgccgaa ctgcaggctg atatcgccac tggcgaccag
    21481 ttcgaccccg gcacggttca ggcgcgagat aacaacgtca tagcgttctt taaagtgcag
    21541 gtcgcgaata cgttttccga gcacgttttc attggtgacc accacacgct cgacgcgcaa
    21601 atcagtgcct ttcgtggaca gcgaagtatc gacctcctga ccaatcacca gttgcgcatt
    21661 atgtaaatcc gctggctgac ccaccagatg cagcaaatcg cccagttgaa taatggtatc
    21721 tggcgaagga acttttaggg tttcttcgcg tttcagacgc gagcagataa ttttgtcgcc
    21781 gttgagaatc ggtacatctt taatggctaa atcatgcagg ttagggttct caacgcgaat
    21841 attgatagtc ttgatcagcg cgccgccatt ggtgcgtgaa gactcgtgct gctgagcttc
    21901 tgtctcgaca ttgacgcgga aaataacccg caacatccac atggtgaaca aaatcccgca
    21961 aatgccgaat ggatacgcca tcgcgtaact catccccatc tgatcgacca tttccattgg
    22021 tgtacccagg tcgcgcaaaa tctgctgccc tgcccccagc gctggcgtat tggtaaccgc
    22081 accggagaaa atccccagca ctaccggcag tggaatatca aacagtttat gcaggatggc
    22141 ggtaaccaga ccaccgatga tgacgatcag aacagcaaac aggttgaggc gtaatccgga
    22201 gacgcgcaat gaggcaaaga agcccggccc tacctgaatc ccgatagtat aaacgaacag
    22261 gatcaggcca aattcctgaa taacatgcag catatcgcta cttaatgtca tccccgcctg
    22321 agaaacaaaa tggccgacga tgatcccacc aaacagcacg ccgccaatac ctaatcctat
    22381 gccgcgaaat ttgacattgc cgataaacaa accgacgact gccaccaaag ccagaatact
    22441 gaccgttaat gctatatcac tcatcgtcca tttctcttga gataacattt ttaattgcta
    22501 cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag
    22561 aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgagtaa gtaagacaaa
    22621 aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac
    22681 gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa
    22741 tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa
    22801 agctcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg
    22861 gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc tctaaatctt
    22921 cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa
    22981 tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt
    23041 caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc
    23101 cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg
    23161 agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc
    23221 gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca
    23281 ggttagcacc acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt
    23341 cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca
    23401 ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag
    23461 cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc
    23521 cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag
    23581 aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag
    23641 aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct
    23701 catcggctac gatgtaaaaa tgggtctgga aatgaacttt tcaagcctta tcagcggact
    23761 tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa cgttaaagtg
    23821 cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga tcgctataac
    23881 tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa tgtgttgtta
    23941 gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt gatgtcccat
    24001 accggaggga aagaaggaac gtatcccggc acccgcgcca gcgcaaccat gattggcgac
    24061 gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt taccgctgtg
    24121 atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagttcggt gcgctcacgc
    24181 gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc ggccagaatg
    24241 cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct tccatccacg
    24301 ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga ttggcgtggt
    24361 tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaagcaatt cgattatcca
    24421 gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga atatccgttt
    24481 tgttatgggt taactggcgc ggctcgccgc cctgtggcga gataagccac agttgcacaa
    24541 tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc
    24601 agtggcgcgg gacgttgact aaccccggat aagcccgatg gtgggtaaag gttaaacgtc
    24661 gctgcacgac gccgcgcggt ggcgcgggca gggtggtttc cgttccactt aacggcgcat
    24721 cacctgccgc tttccagcca gcttcatctt gcggtaactc aacgataaac agctccggca
    24781 ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc
    24841 atccttcttc ataagcacga ttgatttcat cgctgccagg ctgcggcgtg ggcgtggttt
    24901 tactcaccag cacgcaccag tggctaccgc tgtattcacg cggatgctgc ttttgtacgt
    24961 tgaccgggcc aaacggcgca gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt
    25021 catgcattac atggtcgtta taggtaaagc tcaccctttc accgttcggg ctaaagacat
    25081 gcacatggct gccgccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat
    25141 cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg aaatcgtaat
    25201 gccatgtttc atcaggattt tccgggccat ggatgaaaac atatttctct gacttcggat
    25261 gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc
    25321 cggtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca
    25381 cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat
    25441 tacggggagc aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg ggtcccacca
    25501 taatacttca caaggacttc actttcagac aatagtcttt tgccatcgtg atgaatgtga
    25561 gtgaaaagat ggaacatttc gacgtggcga ttattggcct cggcccggca ggatcggcgt
    25621 tggcgcgaaa attagcaggc aaaatgcagg tgatcgcgct ggataaaaag caccagtgtg
    25681 gtactgaagg tttcagcaaa ccttgcggcg gtctgctggc accggacgcg cagcgatctt
    25741 ttattcgcga tggactgacg cttcctgtcg atgtgatcgc caatccacag attttcagcg
    25801 tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc tatatcaata
    25861 ttaatcgcca tgctttcgac ttgtggatga aatcgctgat ccccaccagc gttgaggttt
    25921 atcacgatag cctgtgccga aaaatctggc gtgaggatga taaatggcat gtcatttttc
    25981 gtgcagacgg ctgggagcaa catattaccg cccgctatct ggtcggtgcc gatggcgcaa
    26041 actcgatggt gcggcgacat ctctacccgg atcatcaaat ccgtaaatat gtcgctatcc
    26101 agcagtggtt cgcggagaaa catccggtgc cgttctactc ctgcatcttt gataattcga
    26161 taactaactg ttattcatgg agtatcagca aagacggtta ttttatcttt ggcggtgctt
    26221 atccaatgaa agacggtcag acgcgtttca cgacgctgaa agagaaaatg agcgcctttc
    26281 agttccagtt tggtaaggcg gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct
    26341 ggcaggattt tgtctgcggt aaggacaacg cctttctgat tggcgaagcg gcaggattta
    26401 tcagcgccag ttcgctggag gggattagct atgcgctgga tagcgcagac attctgcgtg
    26461 cggtgttact gaagcagcca gagaagctca ataccgctta ctggcgcgcc acccgcaaac
    26521 tgcgtttaaa actcttcggc aagatagtaa aaagccgatg cctgaccgca ccggctttaa
    26581 gaaagtggat tatgcgcagt ggtatggcgc atattccaca gttgaaagat tagccaacgc
    26641 gcttcacatc gcccaccagc aggatgtaag agagcgcgcc aatcaacgcg acggcggaga
    26701 tataaaccag tgcaggtgcg aaaccgtaac cctgcgccag gtaccccacc accagcggaa
    26761 cggtgatgcc gcccagacca ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc
    26821 gcatcggtgc cagagaagag accagcgacc aggtaatcga agcaaaaccg ttgccgaaga
    26881 atgccagcgc catcaggcac ataatcatca tcggatcgtt ggtgtagtta gcgcccataa
    26941 tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa
    27001 agcccttacg taccagcaga tccgcgaccc agccagagag caggacgccg acaaacgccg
    27061 cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc cgtgattcct ttttcctggg
    27121 ttaaatagtt cgggaaccag gttaagaaaa accacagtgt agaagccacc gcaaattgcc
    27181 caagataaac gccgatcagt ttacgatgga acaccagttt ccagtctttg gctgttaacg
    27241 gctgacgcgc ctctttcttc accggcgcat caccatccac cagaccgccg ccatcacgaa
    27301 tgtaatccag ttcagctttg ctgatacctt tggtcaggcg cggcggctga taaaccttaa
    27361 accaaatcag cgaccagata atgccgatac caccagtgac aatgaacacc cagtgccagc
    27421 tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg acaaactgac
    27481 cagacgtata aaaaccaacg gcggaagcgc gttcatgttc cgggaaccag ctggtcacca
    27541 tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca cgcagaccaa
    27601 ttaatgacat taatcccgtg gcaaagccct ggaataaagt cgccactgac caaccaaata
    27661 tcgcaataaa ataagtcacg cgatctaaaa accaaccgcc ggggatctgg catagcgtat
    27721 aaagccaggc gaaggccgaa aatacatagc ccatttgcgc tttggtaata ccgaactctt
    27781 cctgaatatg ggcggaagcc acggccaggt tagcgcggtc aacataacaa atgaccaccg
    27841 taataaagat catcaccagc gtcagataac gccgacgccc cggttttgct gcattaacgg
    27901 gaatatccat agcgagcttc ctccagattt tgggcatagc gaagccgctc accatgccct
    27961 gtaatttaca gagggttata tttttgtatt gctgttttag tgcccgatga ggggcttacg
    28021 tggcaggaat taccactctg ctacgctgtt atcttcatga cgccagagcg gattacgcca
    28081 gtccggggca tttttactga actcaataac tttagcttcg tcgatttcca cgcctaagcc
    28141 cggtttcgtt agcggtttaa agaagccgcc aaccatgctg aagtcttctt tgtttttcac
    28201 aaagtcgagt aactccgcgc ctttgttgta atgaatgccc atactttgtt cctgaagtac
    28261 ggcgttatag gaaacaaagt cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg
    28321 acagtgcggc gcaagggtca catcataggc ttctgccatt ccggcaattt tgtagcattc
    28381 ggtaataccg cctgcatggg agaggtccgg ttgcagaatc gaaataccac ctgcctccag
    28441 cacgcgttta aaatcgaaac gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt
    28501 ttgtgccgcc agtttcgggt agtattcggc ttgttccgcc agcaccggct cttcaataaa
    28561 cagcgggcga tacggctcca gttctttaat cagcactttc gccattggcg cgctgacgcg
    28621 accgtggaaa tcaagaccaa actcaatctg attgccaaaa gcttcacgaa tttgtgccac
    28681 ggtgttgact gccgcatcta ccgcgcggga gttatcaatt agccccagtt cttcacaacc
    28741 gttcagttta aaggtatcga agccgatttc gcgcagggtt ttaatgccgt cgataacatc
    28801 cgccggacga tcgccgccga cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc
    28861 catcagttgc cagaccggcg cattcagcac tttacctttg atatcccata acgcctggtc
    28921 gatcccggcg atggcgctca tcaggattgg accgccacga taaaatccgg cgcgatacat
    28981 cacttgccat aagtcattga tgcgcgatgg atcctgacca atcaaatagt cacccagctc
    29041 gtgaactgcc gcttccaccg tgcgggcgcg gccttcaatc acgggctcgc cccaaccgac
    29101 cacgccttca tcggtttcaa ttttcaggaa catccagcgg ggaggtaaac gatacgtggt
    29161 aattttggtg attttcattg cactgcctct cgatacgcct taacaaatgc tgctgcctgc
    29221 tgcgcggtgc gttctacgga ctgcccggcg cgatagagat cgctgcctaa gcccgcccca
    29281 gcacaacctg cgtctatcca ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag
    29341 actgcgatgt ccgatggcaa taccgctttt aacgctttga tgtattgcgg accaaaagcc
    29401 gatgacggaa atattttcag cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc
    29461 gccgtcgcgc agccggggca gacggtcatg ccgtagccca ccgcacggcg gatcacttca
    29521 ctatggatat tgggcgtaac gatgagctga cagcccatcc tggcgagcgc atcgacctgt
    29581 tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt cgccatacgc atcaacgatg
    29641 gcgggaatgc tttgctccca ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg
    29701 gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg gcttaatgcc gcgcaaaatg
    29761 gcgatcagcg gaagtttagt ttgccactgc atgagcgatg ctccttatac cagcctgaaa
    29821 tgccgtgtcg cccgccaccg ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta
    29881 gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg tgttgatggg tcacatagtc
    29941 gcgcatactg gcaacctctg cgccaatcaa caaaccagag agaaattcgc tgacctgttc
    30001 gcggggaagt gttcccagca catgcgaggc gcgaacttca aaaagctgcg gcaatatggc
    30061 gggcgcatta aggccgcgct caaggccagc cgcgaaggca tcggcagagt tttcctgcgg
    30121 cggcaaacct gcgccaatca atgagtgatt taacagtaaa tgatgtaatt caccggtcat
    30181 cacggtgcga aaatcgttga tttgctggct atcggcctgc acccatttgc aatgcgtacc
    30241 gggcataaca taaagagagg aaggagccag agctcgcgcg ccgatcaatt gtgtttcttc
    30301 gccgcgcatc acattgtggt tatcgtcatg agagacgcat aatccgggaa taatccagat
    30361 attgtcgcca acagacgtta attgttcgcc aatagacgaa aaacgggcag gaacagataa
    30421 atacggagca actttccagc cgacattgct gccgaccatt cctgccatca ccactggcgt
    30481 attttcttca cgccagtcgg tcgtgacttc tgctaacacc gcagccggag attttccgtt
    30541 caggcgcgtg acgcctgctt ctgattgcct gctctccagg cagtggtcgc cctgataaag
    30601 ccaggcgcgc agattggtcg atccccagtc aattgcgatg tagcgagctg tcatgtgatt
    30661 tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg
    30721 ccgtcctgat gccgtattgc atcgaacagc gccttatgtt cctggagcgt ttgcggcatg
    30781 ttggcctcat cgcccatcca ggttcgttca aaaaccgccc gctgcagcga actgatcgca
    30841 atgctaagtt gctgtaacac cgggttatgc accgactgca gcaccgcctc gtggtagcga
    30901 atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc
    30961 gattcaatct gcgccagatc gctggaagtc gcgcgctctg ctgcccaacg ggcaatcgcc
    31021 ggttccacca gatttcgcac ttcgctcatg gcactgataa gccgtgggtc gtagtcgttt
    31081 tccaacaccc attgcagtac gtcagtgtcg aggtaattcc actggttacg cggtgccaca
    31141 aacgccccgc gataacgttt catttcaatc agccgcttcg ccatcagcga acggaacacc
    31201 tcacggatga tgttgcgcga ggttgcaaac tcttcacaga gctccgcctc agccggaagt
    31261 ggcgagccag gtacgtattt gccgtgaacg atctgtttac ccagcgtaat gacaatgcga
    31321 tcggttttat tgagagtcat ggagagtcct tgtgctctgt atgttcttct ctactttacc
    31381 ccgatcgatg cataacgcgg caactttgta gtaccagcgt gatgacgttc gcgtttgccg
    31441 tgtgagtaat gtagtacaaa cttatattgt tgtactacaa tttagatcac aaaaagaaca
    31501 atgcataaaa aaatgacatg cgtcgggcag aaatctgaaa agggatatca ggcgctaaac
    31561 aggagggaaa gaagagtatg ctttcaacgg cttagctact cgtttaaagg attaatcatg
    31621 aagttgaatt ttaagggatt ttttaaggct gccggtttat tcccactggc gctgatgctt
    31681 tcaggctgta tctcgtatgc tctggtttcc cataccgcaa agggtagttc aggaaagtat
    31741 caatcgcagt cagacaccat cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa
    31801 ggctatgttt ttgtagggga atcgctggat taccttatca ctgatggtgc cgatgacatc
    31861 gttaagatgc tcaatgatcc agcacttaac cggcacaata ttcaggttgc cgatgacgca
    31921 agatttgttt taaatgcggg gaaaaagaaa tttaccggca caatatcgct ttactactac
    31981 tggaataacg aagaagaaaa ggcactggca acgcattatg gttttgcctg tggtgttcaa
    32041 cactgtacca ggtcactgga aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg
    32101 gattactcaa aggtgatggc gttctaccat ccatttaaag tgcgatttta tgaatactat
    32161 tcacccagag gcattccgga tggtgtttcc gcagcattac tgccagtgac tgttacgctg
    32221 gacatcatta ctgcaccgct gcaatttctg gttgtatatg cagtaaacca ataatcagta
    32281 agcgggcaaa cgcgtttatg ctgtttgccc gtccactgat taattcagca catacttctc
    32341 aatagcaaac gccacgccat cttcaaggtt ggatttggtg acaaagttcg ccacttcttt
    32401 caccgaagga atagcgttat ccatcgccac accgacgcct gcatattcaa tcattgcgat
    32461 atcgttttcc tgatcgccaa tcgccatgat ttcttccggt ttaataccta acacgtcggc
    32521 cagtgatttc acccccgtac ctttgttaac gcgtttatcg aggatttcga ggaagtacgg
    32581 cgcacttttc agcacggtat atttctcttt cacttcctgc ggaatacgcg cgatagcctg
    32641 gtcgaggatg gcgggttcat caatcatcat cactttcagg aactgggtat tggggtccat
    32701 tttctccgct tcgcagaaca ccagcggaat ggtggcaacg aaggattcat gcaccgtgta
    32761 gtagctgata tcacggttgg cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga
    32821 accgacttcg cgagagagtt tttccaggaa acgatagtcg tcatagctga gagcagtttg
    32881 cgccacggtg ctaccatcag cggccttctg taccagcgcg ccgttataag taatgcagta
    32941 gtcgcccggc tgttccatat gcagctcttt caggtagttg tgcacacctg catacgggcg
    33001 acccgtcgtt agcacgacat tcacgccacg ggcgcgagct gcggcaatcg catttttaac
    33061 ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca tccatatcga tagcaatgag
    33121 tttgatagcc atgagttccc caggtagatt agttcctgac ccatgctaac gcgattccgc
    33181 tcaaaaatca gtacaacacc cgagggaaaa gggggatgca acgcgcgttg cgtgctcccc
    33241 atttgcttag cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt
    33301 attggcttgt ggagacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg
    33361 ttctgccagt aaactggaag ctgtactggt atccacgcca agtttttgcc cgagatcgga
    33421 caccgcattt gtgccgagcg ccgattccag ttgctcgcca ctaaccgatt gattgccctg
    33481 ttgattactc agccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag
    33541 cagcacctga atgccgccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc
    33601 atcgcctttc agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa
    33661 agcgttacgt gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa
    33721 ataaaaaggg cggagatgat ctccgccctt ttcttatagc ttcttgccag atgcggcgct
    33781 aacgccttac ccggcctaca aaatcatgaa aattcaatac attgcaagat tctcgtaggc
    33841 ctgataagcg tagcgcatca ggcacgctcg catggttaac gccattaaat atcgatattc
    33901 gccgctttca gggcgttctc ttcaataaac gcacggcgcg gttcaacggc gtcgcccatc
    33961 agcgtggtga acagctggtc agcagcaatc gcatccttaa cggtaacgcg cagcatacga
    34021 cgactttccg ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct
    34081 ttataacgct ggatggagag gccgcgacgg gactctttca ccagccagtc cagcgcctgc
    34141 tcgaagctgg ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc
    34201 aagccacgca gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac
    34261 tcgtgatcca gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg
    34321 aacaggtttt gctcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct
    34381 ttgtcgttca gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagag
    34441 aggtcggctt ccgtcagcgt cggctgatag ataagctctt tcagcattgc tttcggataa
    34501 cgacgctcca tgcggttgat cattttctgc gtcgcgttgt actcagacac cagtttctct
    34561 aacgcttcgc cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccatccagc
    34621 gcgatggaga tttggtactg atccatcgct tcgtcgtctt taatatactg ttcctgcttg
    34681 cctttcttca ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt
    34741 tccggcatct gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg
    34801 tccgcatcgg tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca
    34861 cgaccgatac cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc
    34921 ttatcgaagc gcgctttctc aacgttgagg attttaccct tcagcggcag aatcgcctgg
    34981 ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac
    35041 agttcggaaa gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggcccgct
    35101 aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca
    35161 cgggcagcat cgataatttt gccaaccacg attttcgcgt cggttgggtt ttccagcagg
    35221 tattctgcca gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc
    35281 agtttgtctt tggtctggga ggagaatttc gggtccggca ctttcacgga aacgaccgca
    35341 atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct
    35401 tctttgtcca tgtaggcatt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga
    35461 gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca
    35521 tcgttccact gcaacgccac ttcgacgcca ataccgtctt tttcggtgga gaagtagaag
    35581 atattcgggt ggatcggcgt tttgttcttg ttcagatatt caacgaacgc cttgatgcca
    35641 ccttcatagt ggaagtggtc ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg
    35701 ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg
    35761 gtcacattgg tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca
    35821 gtctcgccgg taaccgccag cggggcctgc ggtacaccgt gttcgtagat ctgacggtga
    35881 attttaccct cgcgctggat aaccagctcc agtttttgcg acagggcgtt tactaccgaa
    35941 acaccaacgc cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct
    36001 gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg
    36061 gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg
    36121 ataatttctt tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat
    36181 accatgtggt gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta
    36241 cgcaccgcat ccagcccttt caggactttg atactggagg agtcataaga attcgacatc
    36301 aacgtttctc gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa
    36361 catcttcgaa ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa
    36421 gacctgtgat tgcgtcgctt ttaagcggct ggcaagcaac ccgcgacgct catcatcaag
    36481 ctcagaggca aaatcatcta tcaggtagag acaccgccgc ccgctttcac gggtgaggaa
    36541 ctctccttgc gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt
    36601 atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc gctttgtgcg ggccatgcgc
    36661 ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc agtacctcag catattctgt
    36721 ctctttctcc cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt
    36781 acaggtatca gccatatcgg ccgcgatacc ggcgctgtac tgggcgcgcc aggtgctgat
    36841 ttgctccgcc agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgtgtcac
    36901 ctggcgcagc gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa
    36961 aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc
    37021 gccgttgagt aaagtaaacc cttctggcgt tatcagctgc attggcatca ggtgcgccag
    37081 ttccgcgact ttatgcccgt cagtaccgtc gatgcggact ttgctgtcgc cctgtttgtc
    37141 cttggttaag ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccat ggagaacaaa
    37201 tgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg
    37261 gccgagcgta tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag
    37321 aaagttaaag ccgggagata aagcgagatc cgcggtttca atgttgcgga aatcgcggat
    37381 caacaagcgg gtgagggaca ttacagtctc attggcatga caacataggc agccgactgt
    37441 gatgcggcat cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt
    37501 tcgcatttca gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc
    37561 tccgcaccgc tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg
    37621 gcggtgattt tcagctggtt ttcgctgaca tacaggcgca cgccgcggaa tttctcgtta
    37681 gagagaattg ccgcacgggc aaacgcctgc ttgagcagat cgcagccagc ttccagatgt
    37741 ttgtccggat tcttcggcag aacgcggcgg taatccggga agcgaccatc caccagtttg
    37801 gaggtgaaga taaagtcgcc aacgtgggcg cgaatattgt tgctgccaat ctgcacgcgc
    37861 agcggattgt cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg
    37921 atcaccgaat ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg
    37981 ccgtcggttg ccacggtgcg cagttcttca ccttcggttt caaacagcat accatttaag
    38041 taatagcgaa cgtcctgatg cgccatcgaa aactgggtcg cttcaatcag acgcttcatc
    38101 gttgcctgcg gcagggtaaa ttcgacttca ctctgccagt catcgaggtt cgggaaatcc
    38161 gccgctggca gggtagacag cgaaaaacgg ctacgcccgg agcgtaccag catccgttca
    38221 ccttccagct gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat
    38281 ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc
    38341 accatttcca tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac
    38401 agcagattac cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt
    38461 agcggtttta ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt
    38521 tctgattaaa tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat
    38581 cttacggcag gcatgaagca ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc
    38641 cggcagactg tggttagtca gctctttcgc cagcgccatc gccatctggc gcggacgcgc
    38701 caccgagcgg gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc
    38761 caccgtcttc tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg
    38821 cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cattggcaat
    38881 gacgcggttc agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa
    38941 gaacgccact tcgcccggca aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc
    39001 cacacgggtt tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga
    39061 tttcaaacga tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat
    39121 ctgttgatta ccttccagca gggcgttgaa ggtatggaaa aactcttcct gagatcgttc
    39181 tttattagca aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg
    39241 tttaaactct tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc
    39301 ggagtgcata taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc
    39361 atgcagcagg tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc
    39421 accgccaggg ttatccgcca cctggcgagc cgccgcgcgc gccagttggt tagatttacc
    39481 ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc
    39541 cggggccggg acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt
    39601 ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt
    39661 caccggtttg gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt
    39721 tagcagtcca ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt
    39781 tggcgcgtac agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat
    39841 actgaattct gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga
    39901 aagtgacacg gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca
    39961 tgattgttga cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc
    40021 ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact
    40081 gcgcaagaga tcttctgttt ctcacagatt ttcccgattt atccacagga ctttccagaa
    40141 ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa
    40201 atttaatgac catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct
    40261 tgcgctttac ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg
    40321 gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag
    40381 agaattgact ccggagtgta caattattac aatccggcct ctttaatcac ccatggcttc
    40441 ggtgtccatc gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt
    40501 taggtagaaa tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct
    40561 cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt
    40621 gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa
    40681 gctcgcattt cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca
    40741 gcagccacaa cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg
    40801 gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa
    40861 tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat
    40921 ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga
    40981 agcgttggaa aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc
    41041 attcgggtct atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca
    41101 acctgttcaa gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg
    41161 ttgacggtga aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc
    41221 ccgcccggac catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag
    41281 tcatcgcttt gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc
    41341 cgcaacctca ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg
    41401 accagggcgt accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg
    41461 atctgaccat caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga
    41521 aagagctgaa ctctacccag ccgttccagc tgctggaaac ttcaccgcag tttatttatc
    41581 aggcacagag cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc
    41641 cgctgtataa cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg
    41701 tgccgatgac gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac
    41761 gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg
    41821 aaatctccac ctttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg
    41881 gaagcagcaa cttcgcactg cacaccttcc gcggcgcggc gtactccacg cctgacgaga
    41941 agtacgagaa atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga
    42001 aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg
    42061 acggtaccaa caacttctat accgctaatc tgggtaacgg catcgccgct atcggctata
    42121 aatctcagcc ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg
    42181 ttggcccgga aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg
    42241 attacggttg gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata
    42301 gctttgtggg taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca
    42361 tgtacccgct gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga
    42421 agattcaggc aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagc caggaaatga
    42481 tggcgctgta caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc
    42541 agatgccaat cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg
    42601 caccgtttgc actgtggatc cacgacctgt cggcacagga cccgtactac atcttgccga
    42661 tcctgatggg cgtaacgatg ttcttcattc agaagatgtc gccgaccaca gtgaccgacc
    42721 cgatgcagca gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt
    42781 tcccgtcagg tctggtgctg tactatatcg tcagcaacct ggtaaccatt attcagcagc
    42841 agctgattta ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct
    42901 gattcggtga gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta
    42961 gggcggataa gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg
    43021 acactatcgt agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct
    43081 ccggcttcaa agcccgtgaa gttgccgaaa ccgtgctggg taaactacct aagccgcgct
    43141 acgccgatta tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc
    43201 tatggttccc tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg
    43261 gcggtccggt gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga
    43321 ttgctcgccc tggtgagttt tccgaacgcg cgtttcttaa cgataaactc gacttagccc
    43381 aggccgaggc gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac
    43441 ttaactcgct gcaaggcgca ttctccgcac gggtcaacca tctggtggaa gccctcaccc
    43501 acttgcgcat ttacgtcgaa gcggcaattg atttccctga tgaagagatc gatttcctct
    43561 ccgacggcaa aattgaagcc cagcttaatg acgtgattgc cgaactcgat gcagtgcgtg
    43621 ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa ggtggtgatt gccggacgtc
    43681 ctaacgccgg taaatcgagc ctgttaaacg cgctggcggg ccgtgaagca gcaatcgtaa
    43741 ctgatatcgc cggaaccaca cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc
    43801 cgctgcatat catcgatacc gctgggctac gtgaagccag tgacgaagtg gaacgtattg
    43861 gtatcgagcg cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg
    43921 gcaccacaac agacgccgtg gatccggcag agatctggcc ggaatttatt gcccgtctgc
    43981 cagcgaaact gccgatcacc gtggtgcgca ataaagccga tatcaccggc gaaacgctgg
    44041 gaatgagtga agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg
    44101 tggacgtgct gcgtaaccat ctcaaacaga gcatgggctt tgacaccaac atggaaggcg
    44161 gcttcctggc gcgtcgtcgc cacctacagg cgctggaaca ggcagcggaa catctacaac
    44221 agggcaaagc gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgtc
    44281 tggcacagca gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc
    44341 ggattttctc cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg
    44401 tcagatgcgg cttcgcttca ttgttagcac tcctgttatt cctcaaccct ttttttaaac
    44461 attaaaattc ttacgtaatt tataatctta aaaaaaagca tttaatattg ctccccgaac
    44521 gattgtgatt cgattcacat ttaaacaatt ttagaata
//
LOCUS       DAZGCS010000030        42972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGCS010000030 DAZGCS010000000
VERSION     DAZGCS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.030"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(144..1409)
                     /gene="ybiU"
                     /locus_tag="ACHK01_001725"
     CDS             complement(144..1409)
                     /gene="ybiU"
                     /locus_tag="ACHK01_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO8096692.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(1561..2376)
                     /gene="ybiV"
                     /locus_tag="ACHK01_001726"
     CDS             complement(1561..2376)
                     /gene="ybiV"
                     /locus_tag="ACHK01_001726"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO8096693.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(2522..4954)
                     /gene="ybiW"
                     /locus_tag="ACHK01_001727"
     CDS             complement(2522..4954)
                     /gene="ybiW"
                     /locus_tag="ACHK01_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO8096694.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(4960..5859)
                     /gene="ybiY"
                     /locus_tag="ACHK01_001728"
     CDS             complement(4960..5859)
                     /gene="ybiY"
                     /locus_tag="ACHK01_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO8096695.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            5990..6652
                     /gene="fsa"
                     /locus_tag="ACHK01_001729"
     CDS             5990..6652
                     /gene="fsa"
                     /locus_tag="ACHK01_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8096696.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            complement(6728..7477)
                     /gene="moeB"
                     /locus_tag="ACHK01_001730"
     CDS             complement(6728..7477)
                     /gene="moeB"
                     /locus_tag="ACHK01_001730"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO8096697.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(7477..8712)
                     /gene="moeA"
                     /locus_tag="ACHK01_001731"
     CDS             complement(7477..8712)
                     /gene="moeA"
                     /locus_tag="ACHK01_001731"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO8096698.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASNV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8916..9881
                     /gene="iaaA"
                     /locus_tag="ACHK01_001732"
     CDS             8916..9881
                     /gene="iaaA"
                     /locus_tag="ACHK01_001732"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8096699.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            9868..11739
                     /gene="gsiA"
                     /locus_tag="ACHK01_001733"
     CDS             9868..11739
                     /gene="gsiA"
                     /locus_tag="ACHK01_001733"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO8096700.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            11759..13297
                     /gene="gsiB"
                     /locus_tag="ACHK01_001734"
     CDS             11759..13297
                     /gene="gsiB"
                     /locus_tag="ACHK01_001734"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO8096701.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            13315..14235
                     /gene="gsiC"
                     /locus_tag="ACHK01_001735"
     CDS             13315..14235
                     /gene="gsiC"
                     /locus_tag="ACHK01_001735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO8096702.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            14238..15149
                     /gene="gsiD"
                     /locus_tag="ACHK01_001736"
     CDS             14238..15149
                     /gene="gsiD"
                     /locus_tag="ACHK01_001736"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO8096703.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            15327..17675
                     /gene="pdeI"
                     /locus_tag="ACHK01_001737"
                     /pseudo
     CDS             15327..17675
                     /gene="pdeI"
                     /locus_tag="ACHK01_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17683..19011
                     /gene="dgcI"
                     /locus_tag="ACHK01_001738"
     CDS             17683..19011
                     /gene="dgcI"
                     /locus_tag="ACHK01_001738"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8096704.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(19058..20383)
                     /gene="rimO"
                     /locus_tag="ACHK01_001739"
     CDS             complement(19058..20383)
                     /gene="rimO"
                     /locus_tag="ACHK01_001739"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO8096705.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            20596..20979
                     /gene="bssR"
                     /locus_tag="ACHK01_001740"
     CDS             20596..20979
                     /gene="bssR"
                     /locus_tag="ACHK01_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO8096706.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHMDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            21090..22205
                     /gene="yliI"
                     /locus_tag="ACHK01_001741"
     CDS             21090..22205
                     /gene="yliI"
                     /locus_tag="ACHK01_001741"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO8096707.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22202..22828)
                     /gene="gstB"
                     /locus_tag="ACHK01_001742"
     CDS             complement(22202..22828)
                     /gene="gstB"
                     /locus_tag="ACHK01_001742"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO8096708.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            23075..24277
                     /gene="dacC"
                     /locus_tag="ACHK01_001743"
     CDS             23075..24277
                     /gene="dacC"
                     /locus_tag="ACHK01_001743"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8096709.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(24324..25082)
                     /gene="deoR"
                     /locus_tag="ACHK01_001744"
     CDS             complement(24324..25082)
                     /gene="deoR"
                     /locus_tag="ACHK01_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO8096710.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(25140..25736)
                     /gene="ybjG"
                     /locus_tag="ACHK01_001745"
     CDS             complement(25140..25736)
                     /gene="ybjG"
                     /locus_tag="ACHK01_001745"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8096711.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            26021..27253
                     /gene="mdfA"
                     /locus_tag="ACHK01_001746"
     CDS             26021..27253
                     /gene="mdfA"
                     /locus_tag="ACHK01_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO8096712.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(27294..27578)
                     /gene="ybjH"
                     /locus_tag="ACHK01_001747"
     CDS             complement(27294..27578)
                     /gene="ybjH"
                     /locus_tag="ACHK01_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO8096713.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(27664..28479)
                     /gene="ybjI"
                     /locus_tag="ACHK01_001748"
     CDS             complement(27664..28479)
                     /gene="ybjI"
                     /locus_tag="ACHK01_001748"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO8096714.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(28479..29687)
                     /gene="ybjJ"
                     /locus_tag="ACHK01_001749"
     CDS             complement(28479..29687)
                     /gene="ybjJ"
                     /locus_tag="ACHK01_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096715.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            29771..30307
                     /gene="rcdA"
                     /locus_tag="ACHK01_001750"
     CDS             29771..30307
                     /gene="rcdA"
                     /locus_tag="ACHK01_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO8096716.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(30482..32167)
                     /gene="ybjL"
                     /locus_tag="ACHK01_001751"
     CDS             complement(30482..32167)
                     /gene="ybjL"
                     /locus_tag="ACHK01_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO8096717.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            32437..32814
                     /gene="ybjM"
                     /locus_tag="ACHK01_001752"
     CDS             32437..32814
                     /gene="ybjM"
                     /locus_tag="ACHK01_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO8096718.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(32844..33101)
                     /gene="grxA"
                     /locus_tag="ACHK01_001753"
     CDS             complement(32844..33101)
                     /gene="grxA"
                     /locus_tag="ACHK01_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO8096719.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            33261..33548
                     /gene="ybjC"
                     /locus_tag="ACHK01_001754"
     CDS             33261..33548
                     /gene="ybjC"
                     /locus_tag="ACHK01_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HFO8096720.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            33532..34254
                     /gene="nfsA"
                     /locus_tag="ACHK01_001755"
     CDS             33532..34254
                     /gene="nfsA"
                     /locus_tag="ACHK01_001755"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO8096721.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRQ
                     TIIKESRPFILDYLHKQGWATR"
     gene            34315..35217
                     /gene="rimK"
                     /locus_tag="ACHK01_001756"
     CDS             34315..35217
                     /gene="rimK"
                     /locus_tag="ACHK01_001756"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO8096722.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            35305..35781
                     /gene="ybjN"
                     /locus_tag="ACHK01_001757"
     CDS             35305..35781
                     /gene="ybjN"
                     /locus_tag="ACHK01_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO8096723.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            36133..37245
                     /gene="potF"
                     /locus_tag="ACHK01_001758"
     CDS             36133..37245
                     /gene="potF"
                     /locus_tag="ACHK01_001758"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO8096724.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKKKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            37340..38473
                     /gene="potG"
                     /locus_tag="ACHK01_001759"
     CDS             37340..38473
                     /gene="potG"
                     /locus_tag="ACHK01_001759"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO8096725.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            38483..39436
                     /gene="potH"
                     /locus_tag="ACHK01_001760"
     CDS             38483..39436
                     /gene="potH"
                     /locus_tag="ACHK01_001760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO8096726.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            39433..40278
                     /gene="potI"
                     /locus_tag="ACHK01_001761"
     CDS             39433..40278
                     /gene="potI"
                     /locus_tag="ACHK01_001761"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO8096727.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            40338..40826
                     /gene="ybjO"
                     /locus_tag="ACHK01_001762"
     CDS             40338..40826
                     /gene="ybjO"
                     /locus_tag="ACHK01_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO8096728.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            40867..41994
                     /gene="rlmC"
                     /locus_tag="ACHK01_001763"
     CDS             40867..41994
                     /gene="rlmC"
                     /locus_tag="ACHK01_001763"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO8096729.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(42169..42900)
                     /gene="artJ"
                     /locus_tag="ACHK01_001764"
     CDS             complement(42169..42900)
                     /gene="artJ"
                     /locus_tag="ACHK01_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO8096730.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT        10329 a        10502 c        11359 g        10782 t
ORIGIN      
        1 gtacctgtaa tatattgaat ctgcaggact ttgtaggcca gataagcgta gcgcatcagg
       61 cattaagaaa gcgcacttgt cagcaatctg agtaacgtcg aatcgatttt cttaatccca
      121 catcaattca cttccattaa ctctcaaaca tccatgccca acgctcgctt accgtgaatg
      181 ttgaggtcgg caagcgtaaa gcgtccttcc cagtttgttt catagtcctc gcgcgggaag
      241 tcgcccggcg atgcgccttt ttccagtgcg gccttcacct tgtgcgcgta ggcaagattt
      301 ttctcgcaca tcggtgccgc aggaatgtac atcacgttgc cccaaccctg ttgattttca
      361 acgggggcaa cggaatggat gacgtcgcag tgccaccaga cggagtctcc ggcctcgagt
      421 tttggaatgc tggttaacgc ctcaattaac agtggatgcc attgctctga taccggcaac
      481 actcttccgg gcgctacgcc gcacagttca tcctccggca catcatcaag cagcggacgt
      541 aacagtacgt acgccatggc ttcaggaatg ggcacaacgt gcagcaaccc ctgaccaggc
      601 agcatatcag agagcgctgt ccagccctgg aatgtccgaa acacggaaca tttggtggtg
      661 ttgtccaccg tgtactcttc aacttccgta cgatgtgccg catgccaggg atcatattgc
      721 gccagattgc cattaaagac gttggcgaaa acgcgctgat acgctggaag cagccagcgt
      781 tccagcgccc cggagtcggt atgcgctcca agacctttgg aggtcgttcc gggcggacgg
      841 cggcggatac ggtcagggta gatgacgctc acatccgggt taaaccattg ctttccatca
      901 ctttcaaatg tccacagacg attgagaaac gactgcgcat tcgccatttc ttcactctgg
      961 cgggcctgca tttgcgcctg cgaccagtag atggggtaaa tctcgggacg tgaagcgctg
     1021 agcgtcccga agaaattatc gccggggcct ttgtagacct cgtcaaagcg gttgcggtcc
     1081 agatagtcca gcatcgactg atcccagcct agcgcttgtt cgcgggggaa atggcctttt
     1141 atcaccgcac aaccgcgacg tttaatctgt tcgcgctgct ctgcagtaac atgacctgct
     1201 ttgatatcgg cataagacag caccggccag acggcatcgc cctgtgcttt gagtgcgttg
     1261 atttcagcca ctcgcgtggc aatgtcatcg cttagctgat taaagatctg ctggacgtcg
     1321 ccaagctgcg cccgcagcgc gtgcttcatc tgacggatag ctgctttgtg atcggcaggc
     1381 aatgtgtcgc tggtaaaagt agaagccata accacctctc attaactttc attcgaaacc
     1441 attctgattt gcatgtgaca atataagtta taaataagtt aatgcaagtt taatgatttg
     1501 atgtgatgta caggatgtaa aaagaaggga aaaccggagc gcacggctcc ggtagagagg
     1561 tcagctgtta aaaggggatg tgttatccag caccgcctga atcacattca gcgcgccttc
     1621 atgattatta tcatcggtag cgtaacgggc gatttgttta atgttttccg cagcattgcc
     1681 catcgcaaag gaataacgcg ccattttcag catctccgca tcgttaccgc tgtcgccaat
     1741 cgctaccaca ttttgcggtg acagatccca gcgtttcagt aaccgcgaaa taccgtttgc
     1801 tttatgtaga ccgggaataa tcaggtcgat aaagccaaaa ccactggtga cgggtttcat
     1861 aatgccatcg agcgctatgt gcagtttgtc gatcactaac gggatttgtt catctggcag
     1921 gttgagcgaa aacttgaaca gtacgtcgtc aatctcctga taatctttta cagctttcag
     1981 gcgatggtag tgttttgcca tcagtgcgac aaatgcttcg ggggcatttt cgctgacata
     2041 tgcactttgc agaccgcagg cgacaaaatt gagttgctta tcttttagca actcgccaat
     2101 aacaatccgc gattcatgtc gggtcagttc gccgtggaac agttgcttgc catgttcgta
     2161 aaccagtgcg ccgttttccg cgacaaaaga gatctcatcc tttagctcag gaaagaacga
     2221 aataagctgg taatactgat taccgctggc gacaacgaac ttaatgccgc gctttttcag
     2281 ttcctgatat tgcgccataa aatgtggttg gttgtacgtt ttggcgtcgt taagaaaagt
     2341 accgtccatg tctgtgacga taacttttac gctcataggt gtgctcctgg ctcgaaaatg
     2401 aaaccgtaac agtgtaataa caatgtgacg aagagcacaa attatatttc gaatgaaagt
     2461 aaggatgaaa ttgatgatgt gaatgattta gcccggcgac gacgccgccg ggccgaggag
     2521 attacagcat atgttcagta cgggcgatga tatcgtcctg tgcgtctgga gagagcgcgg
     2581 tgaagaaggc ggaatagccc gcgacacgca ctaccagatc gcggtactga tccggatgtt
     2641 ttttcgcctc cagcagcgtt tcgcgggaaa cgatgttgta ctgaatatgc cagcctttat
     2701 gcacttcaaa gaaggtgcgc agcaggatca tcagtttctg cttgtcagat tcgttctcca
     2761 gcgttgccgg attcagtttc tggttgagca acacgccgcc gagaatcgct gccgtaggca
     2821 gtttacccac cgagccaatg accgcagtag ggccgagatg gtcagtacca gaggccggac
     2881 tagcgccttc tgccagcggg gtgtgggctt tacgcccgtc cggtgttgcc atagtttgcg
     2941 cgccaaacgg tacgttagcg gagatagacg aggtacccgc gtaatagttg ccgccaaccg
     3001 gaccacgacc gtagcgcggg ttatggtact gtttcagttc gtcgatatag gtctgataag
     3061 cgcgagccag cagcgtatcg acggtatcat cgtcgttgcc gtacttcggc gcaccgttaa
     3121 tcagccgctg acgcagttgc tcatgagtca ggccatcgaa gtcatccgcc agtgcggcag
     3181 ccagctgttg ctgaccaatc gcgccctgtt caaataccag tttcttcact gccgccaggc
     3241 tgttgccgag gttggcaatg ccaacctgca ggccagaaac ccagtcatat ttcgcgccgc
     3301 cttgcttgat acttttcgct cgctcaatac agtcatccac cagcgccgag cagagaatat
     3361 cgtgtacgtt ctcttccagc atggtgtcga cgacatattc gatttcgatt gatttacgcg
     3421 tgtaataacg gatttgcgta tcccacgcgt ccattacttc atcgaaattg ttaaagttgc
     3481 ctgccgacaa cgctttttct tgtggcagga acactttgcc gctggtggca tcacgcccgc
     3541 cttccagcgc cgccagcatc acgcgggcga agttgataaa gctcatgccg gtacagcgat
     3601 agccccattt gccaccgacg gcggtttcga tacaaccaat cgctgcgtag tcgtaagcgt
     3661 cctgcggttc aataccgagt ttaataaatt ccgggatcac gatttcgtcg ttgttgaacg
     3721 ccggcatccc gaagccgcaa cggatcacct gtacgcaggc gtcgaggaaa tcattactca
     3781 ttcctgcatg gtaacgcacg ctgaggttag gctgagtgga acgcaggcga ccgcaggatt
     3841 cgaggatcgc gtaagagagg ggattcactg catccattgg ttgaccatca accagatttt
     3901 gtccgccaat ggtaacgttc tgatacagcg gacttcccgc agaggctttt gagtgtgagc
     3961 cggagcggat cttgttcact tccagcagtt tcagccagca gctatgcagc atctcgatgg
     4021 cgtgttcgcg atccagcgtc tggttgagtt caacgtcgcg gcgatagtac gggtagagat
     4081 actggtccat acgaccaaac gataccgagt gaccgttaga ttcgatctgc aaaatcaatt
     4141 ggatgaagta acacagttgc agcgcctgcc agaaagtctg cggcggctgg tgggcgataa
     4201 gatcgcagtt ttctgccatc gtcagcagtt catcgcgacg gctttcgcga gtttcggtag
     4261 cagccatttc acgcgccagg gcagcgaaac gttcaatgtg ttcactgact gccaccagca
     4321 cgatatcaat cgctttcagg aattgttcgc catgtaaatc ttccagcacc gtcagattga
     4381 tgcgcgagcg acgttccgct actttctcac gcagaccatc aagacctttt tccagcagca
     4441 gcgggaaatt caccgctagg tgcgcatcgc cggaggtcat attgccttcc gctttaatga
     4501 ttccggtcgc cagcagacct ttttgctcat cggtaaacat gccgtagcag cgatcctgta
     4561 cggtctgacc gcgccaccac gggcacactt catgcagaac gcgtttgttc tcttcgctca
     4621 ccgcaaagcc agcaccggga cgatctgcca gatcatcaat ctctttttcg atccacgaga
     4681 cagtatattc cgggaagatc ggcgcggcgc gaacttcgct tgcctggttg ccaatgatca
     4741 actcatcgtg tttgatccag atggtgcgat tcgccaggtg atgcgccagt gccagcgcgc
     4801 gacgtaccgg gatcggctta tcgagatgtt gttgatacat ctcggtatag tgctgcgcgc
     4861 gctcggtaca gactggcggt ttcacaatat gcaccagcgc atttttgtgc gctttaatgc
     4921 ggtcgctgag cgtgtccagt ttcagtgtgg tcatggttgt tatcctcgta aggtcgcggt
     4981 taaccctttc tggcaggcat actgctgggc aaagtcgagc agttctggcg catcaagcgg
     5041 tttttccggg gcgtcatagg gcagattaag taagtgatat ttgttgatgc ccagcgtgtg
     5101 gtagggcaga aaatgaattt cgccaacgtg cagctcgtcg gcggcaaaat cggtaatggc
     5161 ttttacagag gtttcgtcgg cattaaagcc ctgaatcagc ggcacgcgga taatgatttt
     5221 tttgcccgct gcggcgagtt ttttcaggtt atccagcact ctggcggcgt taccgtcggt
     5281 ccactgttta aacggcgcgt cggcaacgtg ttttaaatcg gcaagaaaca gatcgatata
     5341 gggcagagaa ggggcgatat atttccacgg cacatgcaga caggtttcta ccgcagtatg
     5401 aatgcctgcc tcgtggctgg cttgcagtag cgccatcgcc atttccggct gcataaaggg
     5461 ctcaccaccc gaaagcgtta aaccgccgcc gctgcgatcg taaaacggtt tatcgcgcag
     5521 aacggtcgtc atgatctcct caacgctttt cacttcacca cacacggtta atgcctgtgt
     5581 cggacagcag tcggttaacg ccgtcagatg ctccggggtt aacttttccc gatgaataag
     5641 caaaccattc agcgcgcgct caatcacttc cggcgcggcc ttagcgcaca gctcgcagcc
     5701 ttccagacac agtcgtgcgt catacagcag atcctgcgtg cgggcgcggc tttccgggtt
     5761 ctgacaccag cggcagccca gcgaacagcc tttaagaaat acgaccgtgc ggataccggg
     5821 gccatcatgg gtcgagtagc gctgaatatt gaaaatcata gttgcctctc tgtttcgttc
     5881 aaacattaaa atactttcga atgaaagtta gattgatgtg cgtcaactgt tcagagagtt
     5941 ttcccgtgat agtctacatt cagacaaaaa gtacattttg aggatggtta tggaactgta
     6001 tctggatact tcagacgttg ttgcggtgaa ggcgctgtca cgtatttttc cgctggcggg
     6061 tgtgaccact aacccaagca ttatcgccgc gggtaaaaaa ccgctggatg ttgtgcttcc
     6121 gcaacttcat gaagcgatgg gcggtcaggg gcgtctgttt gcccaggtaa tggctaccac
     6181 tgctgaaggg atggtgaatg acgcacgtaa attgcgttct attattgcgg atatcgtggt
     6241 gaaagttccg gtgaccgccg aggggctggc agctattaag atgttaaaag cggaagggat
     6301 tccaacgctg ggaaccgcgg tatatggcgc agcacaaggg ctgctgtcgg cgctggcagg
     6361 tgcggaatat gttgcgcctt acgttaatcg tattgatgct cagggcggta gcggcattca
     6421 gactgtgacc gacttacacc agttattgaa aatgcatgcg ccgcaggcga aagtgctggc
     6481 agcgagtttc aaaaccccgc gtcaggcgct ggactgctta ctggcaggat gtgaatcaat
     6541 tacgctgcca ctggatgtgg cacaacagat gattagctat ccggcggtgg aagccgctgt
     6601 ggcgaagttt gaacaggact ggctgggagc gtttggcaga acgtcgattt aaccggacgt
     6661 tctgcatcct cataaattgc tgatgacgtg gcggagtgcc gcgtcttatc tggcctggag
     6721 gtggcaatta ctgcccgcac acctcacacc ccggattgcg catcagtttc atttcgcgaa
     6781 actgacaggt catcgcatcg tacatgacga ttttcccgct ggccggtttg ccataacctg
     6841 ccagcagttt gatcgcttcc attgcctgca acgaaccaat tacgccgatc aacggtgcca
     6901 tcacgcctgc ttctacgcag gttaatgcgt tctcgccaaa caaacggcta aggcagcgat
     6961 agcacggttc gccgtcctga taagtgaaga ccgtgatttg cccttccata cgaattgccg
     7021 cgccggaaac cagcggcacc ttcgcggcaa aacagccagc gttcagttga ttacgcaccg
     7081 caacgttatc cgtacagtcg agtaccagat cgtgttttgc aatcatcgca gcaagttcag
     7141 cgtcatcaag cagtgcattg actggcgtaa tcgcgatatg tgggttgatc cgcgtcatgg
     7201 cgtcacgggc ggattctacc ttcggttgcc cgaccgtagc atcactgtgc agcgtctggc
     7261 gttgcagatt cgagagcgaa accgtgtcga agtcgagcag cgtcaggttg ccgacaccgg
     7321 cgcttgccag atactgcgaa gctgcgcagc cgagtccacc gaggccgact accagcacac
     7381 gagaatcttt tagcgcctcc tgaccgtcaa aatcaaaacc gcgcagaatg atttgccggt
     7441 tgtagcgcag catctcctga tcgctgagtt ccgccattac aggcctccga acaacgcgtt
     7501 aaacggttct acttccaccc attcgcccac gtccacattg ccgcgatcgc gttccagcac
     7561 gataaagcag ttgccgagac taaaggagct aaatatatgt gaaccctgat gtccggtggt
     7621 cgtcacttca agttcaccgt cggcgttgcg ttgcagcacg ccgcgctgga aatcaagacg
     7681 tcctggcgtt tttttcaggc gggacgctgt gcgtacgcgc tggcgcgcgg gcaggccgct
     7741 ggcggtgttg ccgcttagtt ttgccagcaa aggctgtacc agttgataga aggtcagcgt
     7801 cgctgaaacc gggttgcccg gcaggccgca gaaccagcta ttgctgagtt taccgaacgc
     7861 gaacggttta cctggtttaa tcgccagctt ccagaaggcg atctccccca gctcttcaag
     7921 aatcgttttg gtgtaatccg cttcacccac tgaaacaccg ccggaactga tcactacatc
     7981 cgcctggctg tcggcttcaa taaatgcggc gcgcagggca tggggatcgt cgcggataat
     8041 ccctaagttg attacctcgc aacccagctg ttctaacatc aggtgtacgg cgagacggtt
     8101 ggtgtcgtag atttggccgt cgcccagcgg ctgaccgggc aactggagtt catcaccggt
     8161 agaaaaaagc gctacacgca ctttacgaat caccggaact tcggcaatcc ccagtgaagc
     8221 aattactggc agctctgcgg tagtcaggcg agttcccgcc gggaaaacaa ccgcacctgc
     8281 agagatatct tcaccgcgac ggcgaatatt ttgcccgcta cgcacttcag cagtaaaacg
     8341 cacgccattg tccgtttgtt cagtctgctc ctgcatcacc accgcttcgc agccttccgg
     8401 caccggcgca ccggtcataa tacgaatgca ggtgcccgca ggccattcac catggtacgg
     8461 ctgaccggca aaggatttac cggcaacggg cagcggttgc ctggaggcaa tatcggctaa
     8521 acgcaccgcg tagccgtcca ttgcggagtt atcaaacccc ggaacatcaa gcggcgaaac
     8581 gacattgctc gccagaatac gaccaaaaca ctgtaccagt ggcagcgttt cctgggcggt
     8641 cagtggggtg acgcgagaaa gcatctcatt aagcgcggtg tcgagcgaca tcaatccggt
     8701 ggtaaattcc atgaaaacac tcctgcggag gcaaaatcga atttgcctat tatgtcagaa
     8761 aaacgccaca gactgtatgc cacctcgggc gtagcgttgg ctcctgcctt tacatgccat
     8821 atccatcttt ctatattcaa aaattgaatg agtaattcat aaaaattctg atatttatag
     8881 caaaagtggc gagccaccat taatggacga atactatggg caaagcagtc attgcaattc
     8941 atggtggcgc aggtgcaatt agccgcgcgc agatgagtct gcaacaggaa ttacgctaca
     9001 ttgaggcgtt gtctgccatt gttgaaaccg ggcagaaaat gctggaagcg ggcgaaagtg
     9061 cgctggatgt ggtgacggaa gcggtgcgtc tgctggaaga gtgtccactg tttaacgccg
     9121 gaattggcgc tgtctttacg cgtgatgaaa cccatgaact ggacgcctgt gtgatggatg
     9181 gtaacaccct gaaagccggt gcggtggcgg gcgttagtca tctgcgtaat ccggttcttg
     9241 ccgcccggct ggtgatggag caaagcccgc atgtgatgat gattggcgaa ggggcagaaa
     9301 attttgcgtt tgctcgtggc atggagcgcg tctcgccgga gattttctcc acgcctttgc
     9361 gttatgaaca actactggca gcgcgcaagg aaggggcaac cgtcctcgac catagcggtg
     9421 cgccactgga tgaaaaacag aaaatgggca ccgtgggggc cgtggcgttg gatttagacg
     9481 gcaatttggc ggcagccacg tccacaggcg gaatgaccaa taaattaccc ggacgagttg
     9541 gcgatagtcc cttagtgggt gccggatgct acgccaataa cgccagtgtg gcggtttctt
     9601 gtaccggcac gggcgaagtc ttcatccgcg cgctggcggc atatgacatc gccgcgttaa
     9661 tggattacgg cggattaagt ctcgcggaag cctgcgagcg ggtagtaatg gaaaaactcc
     9721 ctgcgcttgg cggtagcggt ggcttaatcg ctatcgacca tgaagggaat gtcgcgctac
     9781 cgtttaacac cgaaggaatg tatcgcgcct ggggctacgc aggcgatacg ccaaccaccg
     9841 gtatctaccg tgaaaaaggg gacaccgttg ccacacagtg atgaacttga tgccggtaat
     9901 gtgctggcgg ttgaaaatct gaatattgcc tttatgcagg accagcagaa aatagctgcg
     9961 gtccgcaatc tctcttttag tctgcaacgc ggtgagacgc tggcaattgt tggcgaatcc
    10021 ggctccggta agtcagtgac tgcgttggca ttgatgcgcc tgttggaaca ggcgggcggt
    10081 ttagtacagt gcgataaaat gctgttgcag cggcgcagtc gcgaagtgat tgaacttagc
    10141 gagcagagcg ctgcacaaat gcgccatgtt cgcggtgcgg atatggcgat gatatttcag
    10201 gagccgatga catcgctgaa cccggtattt actgtgggtg aacagattgc cgaatcaatt
    10261 cgtctgcatc agaacgccag tcgtgaagaa gcgatggtcg aggcgaagcg gatgctggat
    10321 caggtacgca ttcctgaggc acaaaccatt ctttcacgtt atccgcatca actctctggc
    10381 gggatgcgcc agcgagtgat gattgcgatg gcgctgtcat gccgcccggc ggtgctgatt
    10441 gccgatgagc caaccaccgc gctggatgtc actattcagg cgcagatcct gcaattaatc
    10501 aaagtattgc aaaaagagat gtcgatgggc gttatcttta tcactcacga tatgggcgtg
    10561 gtggcagaga ttgccgatcg ggtactggtg atgtatcagg gcgaggcggt ggaaacgggt
    10621 accgtcgaac agatttttca tgcaccgcaa catccttaca cccgtgcgct gttagctgct
    10681 gttccgcaac ttggtgcgat gaaagggtta gattatcccc gacgtttccc gttgatatcg
    10741 cttgaacatc cagcgaaaca ggcccccccc atcgagcaga aaacggtggt ggatggcgaa
    10801 cctgttttac gagtgcgtaa tcttgtcacc cgtttccctt tgcgcagcgg tttgttgaat
    10861 cgcgtaacgc gggaagtgca tgccgttgag aaagtcagtt ttgatctctg gcctggcgaa
    10921 acgctatcgc tggtgggcga gtctggcagc ggtaaatcca ctaccgggcg ggcgttgctg
    10981 cgcctggtcg aatcgcaggg cggcgaaatt atctttaacg gtcagagaat cgataccttg
    11041 tcacccggca aacttcaggc attgcgccgg gatattcagt ttatttttca ggacccttac
    11101 gcttcgctgg acccacgtca gaccatcggt gattcgatta tcgaaccgct gcgtgtacac
    11161 ggtttattgc caggtaaaga cgcggctgca cgcgttgcgt ggttgctgga gcgcgtgggc
    11221 ctgttacctg aacatgcctg gcgttacccg catgagtttt ccggcggtca gcgccagcgc
    11281 atctgcattg ctcgcgcgtt ggcattgaat ccaaaagtga tcattgccga cgaagccgtt
    11341 tcggcgctgg atgtttctat tcgcgggcag attatcaact tgttgctcga tctccagcgt
    11401 gatttcggca ttgcgtatct gtttatctcc cacgatatgg ccgtggtaga gcggattagt
    11461 catcgtgtgg cggtgatgta tctcggacaa attgttgaaa ttggtccacg gcgcgcggtc
    11521 ttcgaaaacc cgcagcatcc ttatacgcgt aaattactgg cggcagttcc ggtcgctgaa
    11581 ccgtcccgac aacgaccgca gcgtgtactg ctgtcggacg atcttcccag caatattcat
    11641 ctgcgtggcg aagaggtggc agccgtctcg ttgcaatgcg tcgggccggg gcattacgtc
    11701 gcacaaccac aatcagaata cgcattcatg cgtagataac attcaggcgg agaataaaat
    11761 ggcaagagct gtacaccgta gtgggttagt ggcgttgggc attgcgacag cgttgatggc
    11821 atcttgtgca ttcgctgcca aagatgtggt ggtggcggta ggatcgaact tcaccacgct
    11881 cgatccgtat gacgcaaatg acacgttatc tcaggccgta gcgaaatcgt tttaccaggg
    11941 gctgtttggt ctggataaag agatgaaact gaaaaacgtg ctggcggaga gttataccgt
    12001 ttccgatgac ggccttacat acaccgtgaa attgcgggaa ggaattaaat tccaggatgg
    12061 caccgatttc aacgcagcgg cggtaaaagc gaatctggac cgggccagcg atccggcgaa
    12121 tcatcttaaa cgctataacc tgtataagaa tattgccaaa acggaggcga tcgatccaac
    12181 aacagtaaag attactctca aacagccgtt ctcagcgttt attaatattc ttgcccatcc
    12241 ggcgaccgcg atgatttcac cggcagcgct ggaaaaatat ggcaaggaga ttggttttca
    12301 tccggtggga accggaccgt atgaactgga tacctggaat cagaccgatt ttgtgaaggt
    12361 gaaaaaattc gcgggttact ggcagccagg attgcccaaa ctggacagca taacctggcg
    12421 tccggtggcg gataacaaca cccgcgcggc aatgctgcaa accggtgaag cgcagtttgc
    12481 tttccccatt ccctacgagc aggccacact gctggagaaa aacaaaaata tcgagttgat
    12541 ggccagtccg tcaattatgc agcgttatat cagtatgaac gtgacgcaaa agccgttcga
    12601 taacccgaag gtccgtgagg cgctgaatta cgccattaac cgtccggcgc tggtgaaagt
    12661 tgcctttgcg ggctatgcaa cgccagctac tggtgtggta ccgccaagta tcgcctacgc
    12721 gcaaagttat aaaccgtggc cttacgatcc agtgaaagcg cgcgaattac tgaaagaggc
    12781 gggatatccc aacggtttca gtaccacgct gtggtcgtca cataaccaca gcaccgcgca
    12841 gaaagtgctg caatttaccc agcagcagtt agcgcaggtc gggattaaag cccaggtgac
    12901 tgcgatggat gccggacagc gggcggcaga agttgaaggt aaagggcaaa aagagagcgg
    12961 cgtgcggatg ttctacactg gctggtcggc ttcaaccggc gaagccgact gggcactatc
    13021 gccgttgttt gcctcgcaaa actggccacc gacgctgttt aataccgcgt tttacagcaa
    13081 taaacaggtg gatgacttcc tggctcaggc actgaaaact aatgatccgg cggaaaagac
    13141 ccgtttatat aaggcggcgc aggatatcat ctggcaagag tcgccgtgga tcccgctggt
    13201 ggtagaaaaa ctggtgtcgg cacacagtaa aaacctgacc ggtttttgga tcatgccaga
    13261 caccggcttc agctttgaag atgcggattt gcaataagca acgcagggag tggaatgctt
    13321 aattacgtta tcaaacgatt actggggttg attccgacgc tgtttatcgt ctcggtgctg
    13381 gtgtttttat ttgtccatat gctgcccggc gatccggcgc gattgattgc cgggcccgaa
    13441 gctgatgcgc aggttataga actggtgcgt cagcagctgg ggttggatca gccgctgtat
    13501 caccagttct ggcactatat cagcaatgct gtgcaggggg attttggcct gtccatggtg
    13561 tcgcgtcgtc cagttgccga tgagattgcc agccgcttta tgccaacgct gtggctgacc
    13621 ataaccagta tggtctgggc ggtgatattt ggtatggcgg cgggaattat cgccgccgtc
    13681 tggcgtaacc gttggccgga tcgattgagt atgaccattg cggtgtcggg gatctcgttt
    13741 ccggcatttg ctctggggat gcttttaatt caggtattct ccgttgaact gggctggctg
    13801 cctactgtgg gagcagacag ttggcagcac tacattttac cctccctgac gctcggcgcg
    13861 gcagtggccg ccgtgatggc gcgctttacc cgtgcgtcat ttgtcgacgt tttaagcgaa
    13921 gattatatgc gtaccgcgag ggcgaaaggg gtgagcgaaa cctgggttgt cctcaaacac
    13981 gggctacgca acgcgatgat cccggtagtg accatgatgg gcttacagtt tggctttttg
    14041 ctcggtggat ctatcgtggt ggagaaagtt ttcaactggc ccggacttgg acgcttactc
    14101 gttgactccg tagaaatgcg cgattacccg gtgattcagg cggaaattct gcttttctcg
    14161 ctggaattta ttcttatcaa cttagtggtg gatgtgcttt acgccgccat taacccggct
    14221 atcaggtaca agtaaggatg cgactattta actggcgacg tcaggcggtg ttaaacgcca
    14281 tgccactggt caaacctgac caggtgcgta caccgtggca tgaattctgg cgacgatttc
    14341 gccgtcagca tatggcgatg accgccgcat tattcgttat tttattgatt gtggtggcca
    14401 tttttgcacg ctggatcgct ccctatgacg ccgaaaatta ttttgattat gacaacctga
    14461 ataacggacc ttctttgcag cactggtttg gcgtcgattc actggggcgc gacattttca
    14521 gccgtgtcct ggttggtgcg caaatctcgc tggcggcggg cgtgtttgcc gtgtttatcg
    14581 gtgcggcgat cgggacgttg ctgggcttgt tggctggata ttatgaaggc tggtgggatc
    14641 ggttgatcat gcgcatttgc gatgtgctgt ttgccttccc gggtatttta ctggcgatcg
    14701 ctgttgttgc ggtgttggga agcggcattg ctaacgtgat tatcgcagtc gccatttttt
    14761 ccatccccgc gtttgcccga ctggtgcgcg gcaacacgct ggtgttgaaa cagcaaacct
    14821 ttattgagtc agcacgcagt attggtgcca gcgatatgac cattttattg cgtcatatct
    14881 tgcctgggac cgtctcgtct atcgtggtgt ttttcaccat gcgcattggc acctcgatta
    14941 tctctgccgc cagcctgtca tttctcggcc tcggtgcgca gccgccgaca ccagagtggg
    15001 gagcaatgct caatgaggca cgagcggata tggttatcgc gccgcatgtc gctgtttttc
    15061 cggccctggc tatttttctg accgtactgg cgttcaattt gttgggcgat ggtttacgcg
    15121 atgcgctgga tccgaaaatt aaaggatagt tacgtttgaa tattgcgtga aagggtaatc
    15181 acctcacagg aaattattgc cctaagcaag tgttgtaact ttctgctgat tttgtagaat
    15241 cgggtaattt ggttaaaaag ccgcagcaag ggactatttt tgcagcggca cagcgttcag
    15301 atagttattt tgttaaatgt attaacatgc tgagtttata cgaaaagata aagataaggc
    15361 tgataatttt atttttattg gcagcactgt catttattgg tctttttttc atcattaact
    15421 atcaactggt atcggaacgc gcggtaaaac gtgccgatag tcgctttgaa cttattcaga
    15481 aaaacgttgg ctatttcttt aaagatattg aacgttcggc cctgacatta aaggactcac
    15541 tgtatttatt aaaaaataca gaggagattc aacgcgccgt gattctgaaa atggaaatga
    15601 tgccattttt agactcggtg ggactggtac ttgatgataa taaatattat ctcttttcgc
    15661 ggagggcgaa tgataaaatc gttgtttatc atcaggaaca agtaaatgga ccgcttgtcg
    15721 acgagtcagg gcgggttatt tttgccgatt ttaacccatc gaaacgaccg tggtcggtgg
    15781 cttcagatga ctctaacaac agctggaatc cggcatacaa ttgctttgat cgtccgggta
    15841 aaaaatgtat ctcttttacg ctacgtatca acggcaaaga tcacgatttg ttagcggtag
    15901 ataaaattca tgtcgattta aactgacggt atctgaacga gtatcttgat caaatcagcg
    15961 ctaatgatga agttctattt ttgaaacaag gtcatgagat cattgccaag aatcaactcg
    16021 ctcgtgaaaa actgattatt tataatagcg aaggtaatta taatattatt gattctgtcg
    16081 atacggagta tattgcaaag acatcagcag tgccaaataa tgcattgttc gaaatctatt
    16141 tttattatcc tggcggtaat ttattgaacg catcagataa acttttttat ctaccgtttg
    16201 ctttcattat tatcgtgttg ctggtggtat atttcatgac cactcgtgtg ttccgtcggc
    16261 aattttctga aatgaccgag ctggttaata cgctggcgtt tttgcccgac tcaacggatc
    16321 aaatcgaggc tctgaaaatt cgcgaaggcg atgcgaaaga gattatcagc atcaaaaatt
    16381 cgatcgcgga aatgaaagat gccgaaattg aacggtcaaa taaattgctc tcactgatct
    16441 cttacgatca ggaaagcggt tttattaaaa atatggcgat tattgagtcc aacaataatc
    16501 agtatctggc tgtggggatc atcaaactgt gtggtctgga agccgtggaa gcggtgtttg
    16561 gtgttgatga acgcaataaa atcgtcagga aattgtgtca gcgaattgcc gagaaatatg
    16621 cgcaatgctg cgatatcgtg acattcaatg ccgatctcta tttacttctg tgtcgggaaa
    16681 atgtacagac atttacccgt aaaatagcga tggtaaacga ttttgacagc agctttggct
    16741 accgcaatct gcgcatccat aagtctgcca tttgtgaacc tttgcagggg gaaaacgcct
    16801 ggagttacgc agaaaaactg aaactggcga tttccagtat ccgtgaccat atgttctccg
    16861 agtttatttt ttgtgatgac gcgaaactca acgaaataga agagaatatc tggattgcgc
    16921 gtaatattcg ccatgcaatg gaaattggcg aactattcct cgtctatcaa ccgatcgttg
    16981 atattaacac ccgcgccatt ctgggcgcgg aggcgttgtg ccgttgggtg tctgcggagc
    17041 gggggatcat ttcaccgctg aagttcatta ccattgctga agatatcggg tttatcaatg
    17101 agctgggtta tcagattatt aaaacggcaa tgggtgaatt tagacatttt agtcagcgtg
    17161 cgtcgctgaa ggatgatttc ttactgcata ttaatgtttc gccctggcag ttaaacgaac
    17221 cgcactttca tgagcgtttt accaccatca tgaaagaaaa tggcctgaag gcgaacagcc
    17281 tctgtgttga gatcactgaa accgtgatcg agcgaattaa tgaacatttt tatctcaata
    17341 ttgaacaact gcgtaaacaa ggggtacgga tatcgattga tgactttggc accggtttgt
    17401 caaacctgaa acgtttttat gaaattaatc cagatagcat aaaagtggac tcgcaattta
    17461 ccggcgatat tttcggtact gcgggaaaaa ttgtgcgcat tattttcgat ctggcgcgct
    17521 ataaccggat cccggtgatt gcggaaggcg tagagagcga agacgttgcg cgcgaattaa
    17581 tcaaattagg atgtgttcag gcccaagggt atctgtacca gaaacccatg ccattctccg
    17641 cctgggataa aagtggaaaa ttagtaaaag agtagtttac gtatgtccag aatcaataag
    17701 ttcgtactta cagtcagtct gctgattttt atcatgattt cagcagttgc ctgcgggatc
    17761 tacactcaaa tggtaaagga acgggtgtat agcctgaaac agtccgttat tgatactgct
    17821 tttgcggtgg caaatattgc tgaatatcgg cgtagcgtgg caattgatct tatcaatacg
    17881 ctaaatccca cggaggaaca gctgttggtt ggcttacgca cagcttacgc tgactcggtt
    17941 tcaccctctt acttgtacga tgtcggtcct tatctgattt ccagtgacga atgtattcag
    18001 gtaaaggagt tcgagaaaaa ttattgtgca gatattatgc aggttgtgaa gtatcgacat
    18061 gtcaaaaata cagggtttat ctcttttgac ggtaaaacct tcgtctatta cctctatccg
    18121 gtaactcaca atcgtagtct gatatttttg cttggtctgg agcgtttttc tttactgtca
    18181 aaatcgctgg cgatggacag cgagaacctg atgttctctc tgtttaagaa cggtaaaccg
    18241 gtgaccggtg atgaatataa tgctaaaaac gccatcttca ccgtttcgga agcgatggag
    18301 cacttcgcct atttgccgac cggattgtat gtatttgcgt ataaaaaaga tgtttatttg
    18361 cgggtttgta cattgattat tttctttgcc gcattggtgg cagtgatatc gggtgccagt
    18421 tgcctctatc tggtacgcag agtgattaat cgtggtattg tggagaaaga agccatcatt
    18481 aataaccatt ttgaacgcgt actggatggc gggcttttct tttcggctgc cgatgtcaaa
    18541 aaactctaca gtatgtataa ctcggcgttc ctggacgacc tgaccaaagc aatgggcaga
    18601 aaatcctttg acgaagattt aaaagcgctg ccggaaaaag gcggttattt gtgcctgttt
    18661 gacgtcgata aattcaaaaa cattaacgac accttcggtc atttgctggg cgatgaagtg
    18721 ttgatgaaag tggtgaaaat ccttaaatca cagatcccgg tagataaagg taaagtctac
    18781 cgcttcggcg gtgacgaatt tgcggtgatt tatacgggtg gaacgctgga agagttgcta
    18841 tcgattctaa aagaaatcgt tcatttccag gtgggaagca ttaatttaag taccagtatc
    18901 ggtgtagcac attcaaatga atgtcctacc gtcgaacgct tgaaaatgct ggcggatgag
    18961 cggctgtata agagtaagaa aaacggcagg gcacagatta gctggcagta atcattattc
    19021 gcaggccgga caaatgattt tgcccggcct gaattaatta aacccggcta ccccacaaat
    19081 cgtactcatc ggcgtgctcg actttcacac gcaggatatc acccggctta acgttggttt
    19141 caccattgag ataaaccgcg ccgtcgattt ccggtgcatc tgccatgctg cgaccaattg
    19201 cgccttcttc gtccacttcg tcgataatca ccagaatttc acggcccact ttctcttgca
    19261 ggcgctcggc ggaaatctgc tgctgcagct gcatgaaacg gttccagcgt tcttctttca
    19321 cttcttccgg aacctggtca ggcagggcat tggcgtctgc accttcaacc gggctgtatt
    19381 taaagcagcc aacgcgatcc agacgcgctt ctttcaggaa gtcgagtagc atctggaaat
    19441 cttcttctgt ctcgccaggg aagccgacaa taaaggttga gcgtagggtc agttccgggc
    19501 agatttcgcg ccactgtttg atgcgcgcca gttggcgatc tacagaaccc ggacgcttca
    19561 tcagtttgag aatgcgcggg ctggcgtgct gcaacggaat gtccagatac ggcaggattt
    19621 tgccttctgc catcagtggg atgacgtcgt ccacatgcgg ataagggtaa acgtagtgca
    19681 gacgtgtcca gatccccagt ttcgataact gttcgcacag gctgaccatg ctggttttta
    19741 ccggctcgcc gttgtggaag ccagtacgat gtttaacatc aacgccatag gcggaagtat
    19801 cctgcgagat caccagaatc tctttaacgc ccgcatctac cagacgtttc gcttcactta
    19861 acacttcgcc aatcggacgg ctcaccaggt cgccgcgcat agacggaata atgcagaagg
    19921 tgcagcggtg attacagcct tctgaaattt tcagataggc ataatgacgc ggtgtcagtt
    19981 tcacaccttg ttctggcacc aggctcagga atgggttgtg tttcggtttt ggcacgtagt
    20041 gatgaacgtg ctccagaacc tgctcatagc tatgaggccc ggtgatttcc agcactttcg
    20101 ggtggacttc gcggatctga tcttcttttg cccccagaca accggtcaca attaccttgc
    20161 cgttttcatt caacgcttca ccaatggctt ccagtgattc ttgtaccgcg ctgtcaataa
    20221 agccgcaggt gttgacgatc accatgtccg catcgtcata gctcggtacc acgtcataac
    20281 cttcagtgcg tagttcggtg agaatacgct ctgaatcgac aaggtttttc ggacagccaa
    20341 gggaaacaaa gccgattttc ggctggggag ttactttgct catagcttaa aaaatattca
    20401 gttacaggaa aggtcagggc agggattcta cagagttctg gataaaattt gtatcgcaat
    20461 ctcattcgct ggcggaggcg aaggaaatgt aaattttgtt aatttggcgt gaagaattga
    20521 tcctggacag cattttgctc aaaaaatagc catactattt aattgcaaca aggctggaaa
    20581 gaggaggatc gaagtatgtt cgttgacaga cagcgaatcg atctgctgaa ccggttgatc
    20641 gacgcacgcg ttgacctcgc cgcatacgtg caactgagga aggcaaaagg atacatgtcc
    20701 gtcagcgaaa gcaatcatct acgagataac ttttttaaac tgaatcgcga actgcacgat
    20761 aaatcgctgc ggttgaatct tcatatggat caggaagagt ggagtgctct tcatcatgct
    20821 gaagaagcat tagcgacagc cgcagtatgt ttgatgagtg ggcaccatga ttgcccgact
    20881 gttattaccg tcaacgccga taagcttgaa aattgtctga tgagcttaac gctgagtatc
    20941 cagagcctgc agaagcacgc catgcttgag aaggcctgaa aactaagggg gagaaagcgt
    21001 ctcccccttc atgtttaagt ttttgtaaaa atgaatttgt tatctcctcc actgactacg
    21061 ctttaagcca gagtcaatcc ggaggcgtta tgcatcgaca atcctttttc cttgtgcccc
    21121 ttatttgtct ttcttccgct ctctgggcgg ctcctgcaac ggtaaatgtc gaagtactgc
    21181 aagacaaact cgaccatccc tgggcactgg cctttttacc cgataatcac ggtatgttaa
    21241 tcactctgcg cggcggcgag ttgcgtcact ggcaagtagg aaaaggatta tctgcgccgc
    21301 tttccggagt tccggacgtt tgggcgcacg ggcagggcgg cctgctggac gtggttttag
    21361 cgcctgattt tgctcagtct cgccgcatct ggttaagtta ttccgaagtt ggcgatgatg
    21421 gcaaagccgg aactgctgtg ggttatggcc gcttaagtga tgatctctca aaagtgaccg
    21481 acttccgcac cgtcttccgc cagatgccaa aactgtctac cggcaaccat tttggcgggc
    21541 ggctggtatt cgacggtaaa ggttatcttt ttattgctct gggcgaaaac aatcagcgcc
    21601 cgacggcgca ggatctggat aaattacagg gcaaactggt gcgtctgacc gaccagggcg
    21661 aaatcccgga tgataatcct tttataaagg aatccggtgc gcgcgccgag atctggtctt
    21721 atggcattcg taatccgcaa ggaatggcga tgaatccgtg gagtaatgca ctgtggctga
    21781 atgaacatgg cccgcgcggt ggtgatgaaa ttaatatccc gcaaaaaggc aaaaactacg
    21841 gctggccgct ggcaacctgg ggaatcaact attcaggctt taagataccg gaagcgaaag
    21901 gggagatcgt cgccgggacc gagcaacctg ttttttactg gaaagattcg cccgctgtga
    21961 gcggcatggc cttctataac agcgataaat tcccccagtg gcagcaaaaa ttatttatag
    22021 gcgcgctgaa agataaagat gtcattgtga tgagcgtcaa cggcgacaaa gtgacagaag
    22081 atggccgtat tttaacggac agagggcagc gaattcgtga tgttcgctct ggacccgacg
    22141 gttatttata cgttctcacc gacgagtcca gtggggaatt acttaaagtt agcccacgca
    22201 attagctaac gggaatcatc accactttgc gcactgcggg gcgttcagtg agttgctgat
    22261 accagcgttg cagatttgga cgcggtgtcc aggtcaggcc aacgttaaac aaattgtaga
    22321 tgaacggtgc gatagcgata tcgcccacac caaactcgtc gccagagaac cattttactt
    22381 ttgccagttc cgcatcgagc agggcaaaca gggcgtcgca ctctttgcaa ctggcatcaa
    22441 tggcggcctg atcgcgctct tcgggtggtg ttctgactaa tcccatcagg atcccgcgat
    22501 gagcattact gagtgtctgg tttgcccagt ccatccattt ttccgcttcc gcacgacgtg
    22561 ccggtgagtc gatccacagg cgtttttgcc cgtactgtgc cgccagatag cggacaatgg
    22621 cgtttgattc ccaaagaatg agatcacttt cgtcgtcacg caacaacggc accagcccgt
    22681 tagggttcat cgccagaaaa tcagcatcgt gatttatccc aaactcacgg cccgcgagaa
    22741 tttgctcata aggtagttcc agttcttcga gcgtcagcaa tacttttttt acgttagttg
    22801 aattattccg accccacagc gtaatcatac tcaccccttt tccgacaggc agccacaggc
    22861 tgaacaggac tccgatggtg aagtaaagtt aacttttaag caacagctgg caaaaaaatg
    22921 acaccagaag cagcgaagcg gcaggatatt gcataaactt taaaaacttt accaacttac
    22981 ggtttcttta agtttgtgtg tgcgttatta atcaccgaac ttatcatacg gcgatataac
    23041 gtattttttt tgaatggata ctcgggtggc atttatgacg caatactcct ctctccttcg
    23101 tggtcttgca gcgggttctg catttttatt cctttttgcc ccaacggcat tcgcggcgga
    23161 acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg attttaatgg attacgccag
    23221 cggtaaagtg ctggcagaag gcaacgcgga tgagaaactg gatcccgcga gcctgactaa
    23281 aatcatgacc agctatgtgg ttgggcaggc gcttaaggcc gataagatta aactcaccga
    23341 tatggtgacg gtcggtaaag atgcctgggc gacgggaaat ccggcactgc gtggttcatc
    23401 ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca gacttgaaca aaggtgtgat
    23461 tatccagtcc ggtaatgacg cctgtattgc gctggccgat tacgttgccg ggagccagga
    23521 gtcatttatt ggtttgatga atggttatgc caaaaaactg ggtctgacca acactacctt
    23581 ccagacggtg cacggtctgg atgcgccggg gcagttcagt accgcgcgcg atatggcatt
    23641 gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac gccattcata aagagaaaga
    23701 gttcaccttc aacaaaattc gtcagcctaa ccgtaaccgt ctgctgtgga gcagcaatct
    23761 gaatgttgat ggcatgaaga caggaaccac ggcaggcgcg ggatataatc tggttgcttc
    23821 ggctacccag ggcgatatgc gtttaatctc cgtagtgctg ggggcgaaaa ctgaccgtat
    23881 ccgttttaat gagtctgaga aattattgac ctggggtttc cgcttctttg aaaccgtgac
    23941 gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc tggtttggtg ataagagcga
    24001 agtgaatctt ggggcaggtg aagcgggctc agtgaccata ccgcgtgggc agctgaaaaa
    24061 cctgaaggcg agctacacgt taacggaacc gcagcttacc gcaccgctga aaaaaggtca
    24121 ggttgtcggg accattgatt tccagcttaa cggtaaatcc attgagcagc gtccgctgat
    24181 cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt tcgtgatgat
    24241 gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc ggatggcgcg
    24301 aaacgtcatc cggttatacg tcattaatac atcaacttaa tacggtgcgt ctgcgcgtac
    24361 ttcacatact catcttccgg gcaacaatca ctcaccacaa tatcaaagcg tttcaggtca
    24421 cccatgcgcg ccggacgcac cttgccaaat ttactgtggt cgacaaccag cacatgcttt
    24481 tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa acaggtagcg
    24541 cctttactga catgcacgcc cgccgcggaa taaaaagcga tatccgggca aaaattattc
    24601 aacgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa ttcaccaccg
    24661 cacagaaatg cgcggcaatg gggtttctct ttcagcgcca gaaaggtatt tagcgaataa
    24721 caaacggcgg taaaagggat ttcattatca atcgcttcaa taatccacgg cgtggtggtg
    24781 ccacagtcaa aaaagagagt ctgatcgggt tctaccagtg tcgcagccat ttttgccgcc
    24841 cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg gctggcactg
    24901 cgcggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg gttgttcaga
    24961 tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt aagatgtaac
    25021 ttatcgctgc gttttaattc ttgcagcagc tgcccgatac gctcttcgcg acgtgtttcc
    25081 ataatccctc tgaatagtta ttgaagcgag ccgctcaata ctacactttt tagcagagat
    25141 cagtcacgca cccagccttt gcggatcggt aatgcaaaac agaagcgata ccacgattgc
    25201 agacgttgat agagtttatg ccccatcgct tgccagataa tctgagcgct aaggcagcca
    25261 atcatacctg ccagcaatcc accgagcata tccagcggcc agtgcacgcc aagataaacg
    25321 cgcgaccagg caatgacgac ggccagcacc attaaaagtg agccggacca caggcgatgc
    25381 cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac cgtggtcgct tgggaatgag
    25441 tcatccgccg catgatgcag gaagttatag ccgatatttt cgacaaaggg tcggtcgtgc
    25501 ggaaaaagat gtcccatcgt ccaggacaca aacaggctga ccgccagcgc gatagcgatt
    25561 tttatcacca gttgccgttg tgctgtaagc ccccacaacc aaagcaccgc ggccagcagc
    25621 ggtaccacgg taatcaaatc tttagcaata aaaatcgcca acgagatcat ccacggagcc
    25681 gagtctggcg tcgcgttaat aagagagaat agagagagat tcaaattttc cagcataact
    25741 tcccgacgca aagtgattaa aagggagcca atacaggcaa gtcgttgaga ataaagtgca
    25801 ggttaaactg ggtaaagcgg catcgtctta tttccctcaa gcggcctgtt tacggtgggt
    25861 aattgtaacg ggcataggtt aaataaaact taaagaaagc gtagctatac tcgcaataat
    25921 gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg ctttctcgtt agctgtgctt
    25981 ttattaaact ctgcgcgatt attattggcg aagaaattgc atgcaaaata aattagcttc
    26041 cggtgccagg cttggacgtc aggcgttact tttccctctc tgtctggtgc tttacgaatt
    26101 ttcaacctat atcggcaacg atatgattca acccggtatg ttggccgtgg tggaacaata
    26161 tcaggcgggc attgattggg ttcctacttc aatgaccgca tatctggcgg gcgggatgtt
    26221 tttacaatgg ctcctggggc cgctgtcgga tcgtattggt cgccgtccgg tgatgctggc
    26281 gggggtggtg tggtttatca tcacctgtct ggcaatatta ctggcgcaaa acattgaaca
    26341 attcactctg ttgcgcttct tgcagggcat aagcctctgt ttcattggcg ctgtgggata
    26401 cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc aagatcaccg cgctgatggc
    26461 gaacgtggcg ctgattgctc cgctacttgg tccgctggtg ggcgcggcgt ggatccatgt
    26521 gctgccctgg gaagggatgt ttgtcttgtt tgccgcattg gcagcgatct cctttttcgg
    26581 tctgcaacaa gccatgcctg aaaccgccac gcgtataggc gagaaactgt cactgaaaga
    26641 actcggtcgt gactataagc tggtgctgaa gaacggccgc tttgtggcgg gggcgctggc
    26701 gctgggattc gttagcctgc cattgctggc gtggatcgcc cagtcgccga ttatcatcat
    26761 taccggcgag cagttgagca gctatgaata tggcttgctg caagtgccta ttttcggggc
    26821 gttaattgcg ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg tacgttcgct
    26881 gattattatg ggcggctggc cgattattat tggtctgttg gtcgctgctg cggcaacggt
    26941 tatctcatcg cacgcgtatt tatggatgac cgccgggtta agtatttatg ctttcggcat
    27001 tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata tgagtaaagg
    27061 tacggtttct gcggcgatgg gaatgctgca aatgctgatc tttaccgtcg gtattgaaat
    27121 cagcaaacat gcctggctga acgggggcaa cggactgttt aatctcttca accttgtcaa
    27181 cggaattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga tgggaaattc
    27241 tcacgaaggg taaaaaaatg cctgattgct ttgtgcgatc aggcattctc gaattaatgg
    27301 tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc aagatggcga
    27361 acataaggat gcgggcggta agccggagct ggcgcaggag ccacatagac ggtttgcggc
    27421 acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac aaccactttg
    27481 tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt aaagcccatt
    27541 aaaagcgcgc ccaacagtag acaatttttc ataaacattt cgcctgaaat aatgcagcgg
    27601 gttaatttcc gcgaattatg cagccttcat tcaggtattg tgttgccggg cgtgctgccg
    27661 tttttattgg tcaaatggcg cttcgtgttt aagcacttta tcgatcacat ccagtacgcc
    27721 ttcacggtta ttggagcctg cccggtattt tgccgctgcg acgaccgcat tgccggcatt
    27781 ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac cgccatcgcc
    27841 aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc gaaggccatt
    27901 ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt ggaccggcac
    27961 cataatatcg ccgatggcct catgtaatgc tttttgtact tgtggaatca gttcatcgga
    28021 aagattcagg ccaaacttaa agaagatatc ctctaagttg tcaaagttat cgacgtattc
    28081 cagacggtga taatacattt ccgccaccgt tttcatggca tcgtcatatt ttttgagcgt
    28141 ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca gcaaatgttc
    28201 cacgacagta gtaaacgcat ccttcgacag ctcgccatta aaaacatctt tgccttcgct
    28261 cactacccag ccgccgtttt cagccacaaa ggcaatttca ttagcaattt cagggaagaa
    28321 agagatcaac tgataatatt gattcccgct ggcgactaca aagcgaattc cttgtgcttt
    28381 catttgctga tactgagcca taaaccgctc acggttatag gttttttgat cgcttaagaa
    28441 agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc catcgccgtc
    28501 tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag aataaccagc
    28561 gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata gcccagcagc
    28621 ggcggcccga cgaggaaagc cagataaccg gtcgttgcta ccacactgac gcgggttggt
    28681 gcatcggggc cggtatcgct ggcggcagaa atggtcagcg ggaagcccag cgaggcaccc
    28741 agtccccaga gtacaacaga caccccagcg acccaggcgc tatcgacaaa aataatcagc
    28801 ccaataccca acgcccccat tagcgcactg gcccgaacca cggcaacgcg actgtaacgg
    28861 tcgatgaacc aaccgccagt gaagcgtcca acggtcatcc ccagggtaaa accggcataa
    28921 atcagcgagc cggaagtggg gctaaaaccg tgaccgtcaa ccattaataa gggtaaccag
    28981 tcgttggcag aaccttcggc aaaggccatc gccagcacca caacaccaat cagcagcaac
    29041 tggatatcgc gataaaaagg tacgcctttt tcgccatgct gggtgccatc ggcagcattt
    29101 ttgcccgtac cgtcagggat tgcctgaatg gcgatatata taggcgcgat gcctaccagc
    29161 gccgccaata aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc catcccgaca
    29221 ccagcgcctg ccagcgtgcc caggctataa aaaccgtgca tcatcggcaa aaccgtttta
    29281 ttcatttctc gctcaacggc ggcaccttca acgtttatcg ccacttccgc agaaccaaaa
    29341 cttgccccaa agacgccgag accaacggca aagagcaggg gcgatgtcag ccagagtgcc
    29401 agacttaata tcatcatccc gaccaatgcg caggacatgg tgaccaggat gacattacgt
    29461 gtcccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga accgatcgac
    29521 agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc gcggatagca
    29581 ggcgtacggg ttgcccagga cgccattaac aggcctggca aaaagaagaa cataaacagc
    29641 gcccaggttc ggcgtttcaa tgcattacgt gaagaattta cggtcataga tcacgtcaaa
    29701 ataagaagag ggaagacaac attagcaagg ttgtgtacat ttgtacacaa ttgcagagag
    29761 aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta tccaggccac
    29821 gctggaggcg gtgaaacttt acggaataca tgctgttacg caccgcaaaa ttgctaccct
    29881 tgccggggta ccgttgggat cgatgaccta ctatttttca ggaattgatg agttgttgct
    29941 ggaggcgttc agcagtttta ctgagatcat gtcccggcaa tatcaggcat tttttagcga
    30001 tgttagtgat gctccaggcg catgccaggc tatcaccgat atgatctaca gctcacaggt
    30061 tgcaacgccg gataatatgg agctgatgta ccagctctac gcgctggcta gccgaaaacc
    30121 gctattaaaa acggtaatgc aaaactggat gcagcgcagt cagcaaacgc tcgaacaatg
    30181 gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga cgctgcattt
    30241 tgtcaccgac cgtaagccgc tatcgcgcga ggagattttg aggatggttg agagggttgc
    30301 agggtagtag ataaatttta gataacaaaa aacccaccaa ccttgaaccg aaatggcggg
    30361 gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa ttaagactga
    30421 tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg caacttcagt
    30481 tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc agcaggacgt
    30541 tggcgattgc ataggtgccc gcatagccca gcgccgggat gttactgcga gctgtatcac
    30601 tgatgatctc cattgctggc gcgcaggtgc gtgcgcccat cattgcgccg aacaacagtg
    30661 cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccacg ggcacaagac
    30721 tgacaatcaa tccggcaatc aacatctgac cgccaatcgc gcccagaccg ttattaatac
    30781 cgctaccggc gctcagacca acgcctgcca taaacaccat taagccgaac tctttcacca
    30841 tgcttaatgc accctgagga atgtaaccga aggtcgggtg gttagcacgc ataaagccca
    30901 gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg aatgtgctga
    30961 actggaatgt gatcatcccg atcatcagcc caataacaaa gaaggcgcag aatgccagca
    31021 agtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg gtttttacgc
    31081 ggcgggcatc gccgctgact tgtaaaacgt cacctttgtt aagcacgacg ttgtcatcta
    31141 tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga tcggtcaact
    31201 tcagttgtgc gagacgtttg ccaacggcgt tatggttttt aacgaccact tcttcagtga
    31261 cgatacgcat gtcgagaagg tcacgatcga aaacttcttt gccgttacgg aagctgggat
    31321 cgagtcgggc atgggcgtcg ggatagccta ccaacgctat ttcatcgccc atttgtagca
    31381 cggcatcacc gtctggattt gccagaatcc cgttacgtcg aatacgttca atgtagcagc
    31441 cggtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag gccaccagct
    31501 ccgggccgac gcgataggcg cggatcaccg gtaaataaac cttacggttg gcatcagtgt
    31561 ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga tgctgcaatt
    31621 tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag gttaaggcat
    31681 acccgaggct cagattatcc agtgccagtg agagctgcct gctttccatg ccggaatgac
    31741 gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcct gctaacatac
    31801 cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg atcaccagcg
    31861 cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga aaaaaaatgg
    31921 aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata aagccaagat
    31981 taagcgcatc ggtgttaatg ctgaaatgtt gttggcctaa taacagtgat acgactaaaa
    32041 cgccaatgga attacccagt tggatcgaac caagtcgtaa ctttccgaga catagcccaa
    32101 gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg gcgacgttta
    32161 tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg gtaatttacg
    32221 ataatgtttt ttaccagaat tcagggcgca gattcattca gcgcacctaa acgatagtaa
    32281 agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata tgaattgcgt
    32341 tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca cactggcgaa
    32401 aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca acgttgggcg
    32461 ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc tttttctggc gacgcacatg
    32521 aaaggcgctt ttcgggctgc cgggcatcct gaaatcggct tgctattttt cattcttcct
    32581 ggagcagtcg ccagcttctt ttcacagcgt agagaagtcc tgaaacctct gtttggcgca
    32641 atgctggcgg caccctgttc aatgcttatt atgcggctgt ttttttcacc gacccgctca
    32701 ttctggcaag agctggcatg gttactaagc gcggtgttct ggtgtgcgct gggggcactg
    32761 tgtttcttat ttatcagtag tttgtttaaa ccacagcaca gaaaaaatca gtaaagccct
    32821 caacgcgagg gcttgtcaga cgatcaggcg tccagatttt ctttcaccca tgcagcaaaa
    32881 tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt ttctacgggt
    32941 ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat atctacatac
    33001 tgatactgaa aatcatcgcg ttcattgctc aatttctcag ccagatcttt tgcacgcaca
    33061 cagtaagggc aacccggacg accaaaaata acggtttgca ttatttctct cctcatagat
    33121 ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa cctgttgcat
    33181 taattgctaa aagctataac tgttaaacac aatacagtga aaagttttag actgaaggct
    33241 cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaagc gtgttgatac
    33301 tggaatttat cggaatgatg ttgctggcgg tggcgctgct gtcggtaagc gacaccctgt
    33361 cgctgcctga gccattttct cggccagaag tgcagattct gatgattttt ctcggtgttt
    33421 tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt cttgccccac
    33481 agctgatgaa ccgtccaccg caatattcac gttcagaaag agaaaaagat aatgacgcca
    33541 accattgaac ttatttgtgg tcatcgctcc attcgccatt tcactgatga acccatttcc
    33601 gaagcgcagc gtgaggcgat tattaacagc gcccgtgcga cgtccagttc cagttttttg
    33661 cagtgcagta gcattattcg cattactgac aaagcgttac gtgaagagct ggtgacgctg
    33721 accggcgggc aaaaacacgt agcgcaagcg gcggagttct gggtgttctg tgccgacttt
    33781 aaccgccatt tacagatctg tccggatgct cagctcggcc tggcggaaca actgttgctc
    33841 ggtgtcgttg atacggcaat gatggcgcag aatgcattaa ccgcagcgga atcgctggga
    33901 ttgggtgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac gaaactgctt
    33961 aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc tgcggataat
    34021 ccggatctta agccgcgttt accggcctcc attttggtgc atgaaaacag ctatcaaccg
    34081 ctggataaag gcgcactggc gcagtatgac gagcaactgg cggaatatta cctcacccgt
    34141 ggcagcaata atcgccggga tacctggagc gatcatatcc gccaaacaat cattaaagaa
    34201 agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg ctaaaaccgc
    34261 cacgtcgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg cagggtgaaa
    34321 attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg tgaagccgct
    34381 atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat gaacataaat
    34441 cctgcggcgt cttctattca ctacaaaggc cgcaagttac cccattttga cgcagtgatc
    34501 ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca gttcgagatg
    34561 ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga caaattgcgt
    34621 tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat tgcgcattcg
    34681 ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt ggtcaagttg
    34741 gttgaaggca cgcagggaat tggcgtcgtg ctggcggaga cgcgtcaggc ggcggaaagc
    34801 gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata tatcaaagag
    34861 gcgcaagggt gcgatatccg ctgtctggtt gttggcgatg aagtggtcgc tgcgattgaa
    34921 cggcgggcga aagagggcga ttttcgttcc aatttgcatc gtggcggcgc ggcaagcgtc
    34981 gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac gatggcgctg
    35041 gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat ggaggtgaat
    35101 gcgtcgccgg ggctggaagg aatagaaaaa accaccggta tcgacatcgc gggtaaaatg
    35161 atccgctgga tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg tggttagtcg
    35221 caatcacatt actgatcatg gttttgcctg cgctttttgc gtaagctgtg ccggtctttt
    35281 tatcgaaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct ggatacgctg
    35341 cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg tcaggctctg
    35401 catctgcccc atatgcagaa tttcgacggt gtctttgatg ccaaaatcga tctgatcgat
    35461 aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt gccgctggcg
    35521 gcggatttat ctgccatcaa tgccagttcg ctgaccgtga aagcatttct tgatatgcag
    35581 gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca gggcgtaacc
    35641 tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat ggtcattctt
    35701 gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa caacgttacc
    35761 gaaaactatt ttctccactg ataattcctt tcgagcacgc agtcgctgcc gcagtgactg
    35821 cgcgctgcaa aattatctgc tgtttttaac cttttcttaa agattatttc acttatcttg
    35881 tgtcgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa ataccgtttt
    35941 ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc ttttatatag
    36001 cgagcagtgc tggccgggag agagttctct tttcttacac cgcgccgata aaaaaatatg
    36061 cacgtttatt gcatatcttt cagtgtgaca acttttgttc gtttgttaac gaactttcag
    36121 aaggaaagag atatgaccgc cttaaataaa aaatggctat cgggtctggt tgcgggtgct
    36181 ctgatggccg tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca catttataac
    36241 tggtctgatt atatcgcccc tgacacggtg gccaattttg aaaaagaaac cggtattaaa
    36301 gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat ggcgggtagc
    36361 accggctttg acttggtggt tccatctgcc agctttcttg aacgccagct gacagcggga
    36421 gttttccagc cgctggacaa aagcaaattg ccggagtgga agaatctcga tccggagctg
    36481 ctgaagttgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta tatgtgggcg
    36541 acgaccggga ttggctataa cgttgataaa gttaaagctg tgctgggcga aaacgcgccc
    36601 gtcgatagct gggacttgat cctcaagcct gaaaatctgg aaaaactgaa aagctgcggt
    36661 gtctctttcc tggatgcgcc agaagaagtt tttgctaccg tgttgaatta tctcggcaaa
    36721 gatcccaaca gcactaaagc ggatgattac accggaccgg caaccgatct gctgttaaag
    36781 ctgcgcccga acattcgtta tttccattca tctcaataca ttaacgacct ggcaaacggc
    36841 gatatttgcg tcgctatcgg ctgggcaggt gatgtctggc aggcgtcaaa ccgcgcgaag
    36901 gaagcgaaga atggagtgaa tgtctcgttc tcgattccaa aagaaggggc gatggcgttc
    36961 tttgatgtat tcgccatgcc tgcggatgcc aaaaaaaaag acgaagccta tcagttcctg
    37021 aattacctgc tgcgcccgga tgtagtagcg catatttccg acaatgtgtt ctatgccaac
    37081 gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc gggtatttat
    37141 ccgcctgcgg atgttcgtgc gaagctgttc actctgaaag tgcaggaccc gaaaatcgac
    37201 cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc agccttagat
    37261 gccgggcggg cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat
    37321 tcaatgccgg agagcagccg tgaatgacgc tatccctcgc ccgcaggcga aaacccgtaa
    37381 ggcgctgacg ccgctattag aaatccgcaa cctcaccaaa tcctacgatg gtcaacatgc
    37441 ggtggatgat gtcagcctga ccatctacaa aggtgaaatc ttcgcgctgc tgggcgcatc
    37501 cggctgtggc aagtccacgc tgctgcgtat gctggcaggt ttcgaacaac cttctgccgg
    37561 acagataatg cttgatggcg tcgatttgtc acaggtgccg ccttacctgc gccccatcaa
    37621 tatgatgttt cagtcttacg cgctgtttcc ccatatgact gtggagcaga acatcgcctt
    37681 tggcctgaaa caggacaaac taccgaaagc ggaaattgcc acccgggtca acgagatgct
    37741 tgggctggtg cacatgcagg agttcgccaa acgcaaaccg catcagcttt ccggtggtca
    37801 gcgacagcgt gtggccctgg cccgaagcct tgccaaacgc ccgaaactat tactgctcga
    37861 tgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga
    37921 tattctggag cgcgtcggtg tgacttgtgt gatggtcacc cacgatcagg aagaggcgat
    37981 gaccatggcg gggcgtatcg ccattatgaa tcgcgggaaa tttgtccaga ttggcgagcc
    38041 ggaagagatc tacgagcatc caactacccg ctacagcgct gaatttatcg gttcggttaa
    38101 tgtctttgaa ggtgtactca aagagcgtca ggaagatggc ctggtgcttg attcgccggg
    38161 gctggtgcat ccactgaaag tcgatgcgga tgcttcagtg gtcgataacg tgccggtaca
    38221 cgtagcgctg cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa
    38281 cttcgcggtg ggggaggtga tacacattgc ctatctcggc gatctttcgg tgtatcacgt
    38341 tcgtctgaaa agtgggcaga tgattagcgc ccagctacaa aacgcccatc gtcatcgtaa
    38401 agggttaccg acctggggcg acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt
    38461 gctgacggtt taaggagcaa agatgaatac acttgaacct gctgcccagt cgaaaccgcc
    38521 gggcggattt aagctgtggt tgtcgcagct gcaaatgaag catgggcgca aactggtcat
    38581 tgcattgcca tatatctggt tgatcttgct gtttctgctg ccatttctga ttgtctttaa
    38641 aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga tggagtgggc
    38701 tgacgggcaa ctgtccatca ctcttaatct cggtaatttt ctgcaactga ccgacgatcc
    38761 gctctatttc gatgcttatc tccagtcgct acaggtggct gcgatttcga cattttgctg
    38821 tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa
    38881 tattttatta ctactggtga tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc
    38941 ctggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt ggctgggggt
    39001 tatagaccag ccgctgacca ttctgcatac caatctggcc gtttatatcg gcattgttta
    39061 cgcttacgtg ccgtttatgg tactgccgat ttataccgcg ttgattcgta ttgattattc
    39121 gctggtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct ttactgtgat
    39181 cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta tcccggctgt
    39241 gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga tcgggcgcgt
    39301 gctatggcaa gagtttttca ataaccgcga ctggccggtg gcctcggcgg tagcgatcat
    39361 catgttgctg ctgctaattg tgccgataat gtggttccac aaacaccagc aaaaaagcgt
    39421 gggagaacac ggatgaataa tttaccggta gttcgttcac cctggcggat tgtgattttg
    39481 ctgctgggct ttacctttct ctacgcgcca atgctgatgc tggttatcta ttcgtttaac
    39541 agctcgaagc tggtgacggt gtgggccggc tggtcaacgc gctggtatgg tgagttattg
    39601 cgcgatgacg cgatgatgag tgcggttggt ttaagcctga caattgctgc ctgtgcggca
    39661 acggcggcgg cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt tggcaggttt
    39721 cgcggatcaa atggctttgc ctttatgatc accgcgccgc tggtgatgcc agatgtcatc
    39781 acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg ctggcctgcg
    39841 gaccgcggta tgctcaccat ctggctggcg catgtcactt tttgtacggc ttatgtggcg
    39901 gtcgttattt cttcaagatt gcgggaactg gatcgctcga tagaagaagc agcgatggat
    39961 ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat catgcccgcg
    40021 atcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt gatcgccagc
    40081 tttgtttccg ggccgggagc caccacgtta ccgatgctgg tcttttccag cgtgcggatg
    40141 ggggtgaatc cggaaatcaa cgcccttgca acgttaattc tcggtgcggt cggaattgtc
    40201 ggatttatcg cctggtatct gatggctcgc gcagaaaaac agcggatacg cgatatccag
    40261 cgtgcaagac atggctgaag acactaaaat ttgccaacct ggctacataa tgccgcgcat
    40321 gccgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa acatcttcat
    40381 ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc attattatga
    40441 tccgtggcct cgacgtgctg atgattttca atacgctggg cgtgcgcggt attggcgagt
    40501 tcattcatcg cagcgtacaa acctggagtt taacgctggt ctttttaagc agtctggtgc
    40561 tggttttcat tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct
    40621 ggctatatct gctgacacaa atcaccgccg caagttactt gtgggcggct tcgctggggt
    40681 acggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc
    40741 tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt ccctcgacca
    40801 gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg atgtgaaggt
    40861 caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg
    40921 cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct gctcgccgat
    40981 tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa
    41041 gccaaaatgg tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct gcatcgcgac
    41101 ggcacaccag aagacctttg tgactgcccg ctttatcctg cctcatttgc gcccgttttt
    41161 gcggcgctaa aaccgtttat cgcccgtgcg gggctgacac cctacaacgt ggcgcgtaag
    41221 cgtggcgaac tgaaatacat tctgctgact gaaagccaga gcgatggcgg catgatgctg
    41281 cgctttgtac tgcgttctga aaccaaactg gcacaactgc gtaaggcgct gccgtggtta
    41341 caggaacaac taccgcaact gaaagttatt accgtcaata ttcagccggt acatatggcg
    41401 attatggaag gggagacgga gatctacctt actgaacaac aggcgctggc agagcgtttt
    41461 aatgacgtac cgctgtggat ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc
    41521 agccagctgt acgctaccgc gcgcgactgg gtacggcagc tgccggttaa acatatgtgg
    41581 gatctgttct gcggtgtggg tggctttggt ttacactgcg cgacgcctga catgcagtta
    41641 accgggatcg aaattgcatc agaggccatt gcctgtgcaa agcagtcagc cgctgaactg
    41701 ggcttaacgc gtttgcaatt tcaggcgctg gactccactc agtttgccac cgctcagggg
    41761 gaggtgccgg agctggtgct ggttaatccg ccgcgccgcg gcattggtaa accgctatgt
    41821 gattatctct caacaatggc accgcgtttt attatctact ccagctgtaa cgcccaaacc
    41881 atggcgaaag atatccgcga actgccaggt taccgcatcg aacgggtaca gctttttgat
    41941 atgttcccgc acaccgcgca ctatgaagtg ctgacgctgc tggtgaagca ataaaaaagc
    42001 cgcaggtgcg gcttcagatt gctgatcagg tgcgctttgt tcatgccgga tgcggcgtgt
    42061 acgccttatc cggcctacaa aatcatgcta aatcaataca ttgcagagtt gatgtgggcc
    42121 ggataagcgc agcgcatcag gcaatcttac ttttgtcatc agtctcaatt actgcggaaa
    42181 ccactggtca ttgattttct gatatgtacc gtcagcttta attgctgcca gcgcgttatt
    42241 cagtttttcc agcagagctt tgttatccgg acgtacagcg atgcccagac cggtgccgaa
    42301 gtattgcgga tcggtcactt tttcagtagc aacgcccagt tgcggattgg ttttcagcca
    42361 ttcgtttacc accgctgtgt caccaaatac cccatcaata cgaccatttt tcagatcgat
    42421 aaaggcattc tgataactgt cataggagac agttttcact tcggggtgct gatcctgaat
    42481 atatttctgg tgcgtggtgc cgttttccat cccaatacgt ttgcctttca gatcggcaaa
    42541 cgttttgtag gtatcttttt tggcaatcac gacggctgag ttttcatagt agggcgtggt
    42601 aaacgatacc tgcttgctac gctcgggcgt gatatccata ccggagatta cggcgtcata
    42661 ttttctgaat ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa aagtacattc
    42721 tgcctgcatt tgtttgcaca aggcttttgc cagatcgata tcaaagccga caatctcatt
    42781 attagcacct atagattcaa agggtggata ggtggctgaa acgccaaaat tgattttctc
    42841 tgcggcagaa gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta actttttcat
    42901 gatggaactc ccgtctgtca atcttatgat ttttggccgt gtctgcggca tgggataaca
    42961 atgccatcaa gt
//
LOCUS       DAZGCS010000031        42939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGCS010000031 DAZGCS010000000
VERSION     DAZGCS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.031"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..808)
                     /locus_tag="ACHK01_001765"
                     /pseudo
     CDS             complement(133..808)
                     /locus_tag="ACHK01_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(953..1561)
                     /gene="yiiX"
                     /locus_tag="ACHK01_001766"
     CDS             complement(953..1561)
                     /gene="yiiX"
                     /locus_tag="ACHK01_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO8096731.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1745..2062)
                     /gene="metJ"
                     /locus_tag="ACHK01_001767"
     CDS             complement(1745..2062)
                     /gene="metJ"
                     /locus_tag="ACHK01_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO8096732.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2339..3499
                     /gene="metB"
                     /locus_tag="ACHK01_001768"
     CDS             2339..3499
                     /gene="metB"
                     /locus_tag="ACHK01_001768"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO8096733.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGYGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3502..5934
                     /gene="metL"
                     /locus_tag="ACHK01_001769"
     CDS             3502..5934
                     /gene="metL"
                     /locus_tag="ACHK01_001769"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO8096734.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6073..6207)
                     /locus_tag="ACHK01_001770"
     CDS             complement(6073..6207)
                     /locus_tag="ACHK01_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096735.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6283..7173
                     /gene="metF"
                     /locus_tag="ACHK01_001771"
     CDS             6283..7173
                     /gene="metF"
                     /locus_tag="ACHK01_001771"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO8096736.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7502..9682
                     /gene="katG"
                     /locus_tag="ACHK01_001772"
     CDS             7502..9682
                     /gene="katG"
                     /locus_tag="ACHK01_001772"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO8096737.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9776..10681
                     /gene="yijE"
                     /locus_tag="ACHK01_001773"
     CDS             9776..10681
                     /gene="yijE"
                     /locus_tag="ACHK01_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO8096738.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10708..11325)
                     /gene="yijF"
                     /locus_tag="ACHK01_001774"
     CDS             complement(10708..11325)
                     /gene="yijF"
                     /locus_tag="ACHK01_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO8096739.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11600..12703)
                     /gene="gldA"
                     /locus_tag="ACHK01_001775"
     CDS             complement(11600..12703)
                     /gene="gldA"
                     /locus_tag="ACHK01_001775"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO8096740.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12714..13376)
                     /gene="fsa"
                     /locus_tag="ACHK01_001776"
     CDS             complement(12714..13376)
                     /gene="fsa"
                     /locus_tag="ACHK01_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8096741.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGIVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13388..15889)
                     /gene="ptsP"
                     /locus_tag="ACHK01_001777"
     CDS             complement(13388..15889)
                     /gene="ptsP"
                     /locus_tag="ACHK01_001777"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8096742.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16198..17277
                     /gene="frwC"
                     /locus_tag="ACHK01_001778"
     CDS             16198..17277
                     /gene="frwC"
                     /locus_tag="ACHK01_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO8096743.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17292..17612
                     /gene="frwB"
                     /locus_tag="ACHK01_001779"
     CDS             17292..17612
                     /gene="frwB"
                     /locus_tag="ACHK01_001779"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8096744.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17663..19960
                     /gene="pflD"
                     /locus_tag="ACHK01_001780"
     CDS             17663..19960
                     /gene="pflD"
                     /locus_tag="ACHK01_001780"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8096745.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19926..20804
                     /gene="pflC"
                     /locus_tag="ACHK01_001781"
     CDS             19926..20804
                     /gene="pflC"
                     /locus_tag="ACHK01_001781"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO8096746.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            20806..21147
                     /gene="frwD"
                     /locus_tag="ACHK01_001782"
     CDS             20806..21147
                     /gene="frwD"
                     /locus_tag="ACHK01_001782"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8096747.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21134..21985)
                     /gene="yijO"
                     /locus_tag="ACHK01_001783"
     CDS             complement(21134..21985)
                     /gene="yijO"
                     /locus_tag="ACHK01_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8096748.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22134..23867)
                     /gene="eptC"
                     /locus_tag="ACHK01_001784"
     CDS             complement(22134..23867)
                     /gene="eptC"
                     /locus_tag="ACHK01_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO8096749.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24050..26701)
                     /gene="ppc"
                     /locus_tag="ACHK01_001785"
     CDS             complement(24050..26701)
                     /gene="ppc"
                     /locus_tag="ACHK01_001785"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO8096750.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27053..28204)
                     /gene="argE"
                     /locus_tag="ACHK01_001786"
     CDS             complement(27053..28204)
                     /gene="argE"
                     /locus_tag="ACHK01_001786"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO8096751.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28358..29362
                     /gene="argC"
                     /locus_tag="ACHK01_001787"
     CDS             28358..29362
                     /gene="argC"
                     /locus_tag="ACHK01_001787"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO8096752.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29370..30146
                     /gene="argB"
                     /locus_tag="ACHK01_001788"
     CDS             29370..30146
                     /gene="argB"
                     /locus_tag="ACHK01_001788"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO8096753.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30207..31580
                     /gene="argH"
                     /locus_tag="ACHK01_001789"
     CDS             30207..31580
                     /gene="argH"
                     /locus_tag="ACHK01_001789"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO8096754.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32079..33293
                     /gene="manD"
                     /locus_tag="ACHK01_001790"
     CDS             32079..33293
                     /gene="manD"
                     /locus_tag="ACHK01_001790"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO8096755.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33360..34643
                     /locus_tag="ACHK01_001791"
     CDS             33360..34643
                     /locus_tag="ACHK01_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8096756.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAASPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34895..35812
                     /gene="oxyR"
                     /locus_tag="ACHK01_001792"
     CDS             34895..35812
                     /gene="oxyR"
                     /locus_tag="ACHK01_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO8096757.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35795..37195)
                     /gene="sthA"
                     /locus_tag="ACHK01_001793"
     CDS             complement(35795..37195)
                     /gene="sthA"
                     /locus_tag="ACHK01_001793"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO8096758.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37526..38176
                     /gene="fabR"
                     /locus_tag="ACHK01_001794"
     CDS             37526..38176
                     /gene="fabR"
                     /locus_tag="ACHK01_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO8096759.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38176..38535
                     /gene="yijD"
                     /locus_tag="ACHK01_001795"
     CDS             38176..38535
                     /gene="yijD"
                     /locus_tag="ACHK01_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO8096760.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38575..39675)
                     /gene="trmA"
                     /locus_tag="ACHK01_001796"
     CDS             complement(38575..39675)
                     /gene="trmA"
                     /locus_tag="ACHK01_001796"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO8096761.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39789..39979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40044..41887
                     /gene="btuB"
                     /locus_tag="ACHK01_001797"
                     /pseudo
     CDS             40044..41887
                     /gene="btuB"
                     /locus_tag="ACHK01_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     gene            41832..42689
                     /gene="murI"
                     /locus_tag="ACHK01_001798"
     CDS             41832..42689
                     /gene="murI"
                     /locus_tag="ACHK01_001798"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO8096762.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9789 a        10698 c        11807 g        10645 t
ORIGIN      
        1 aaaatataat atatgtcatt tgagaaaaaa gagcagaatg aggtaaaaat ctttggctgt
       61 gtattcagtc tggtaatcat ccaaggaaag cttatcagaa gggtgatgac tccaattagt
      121 agaacatata ttttcgataa aagcaccgat gactttttca tgaagttcta cagaacgcga
      181 gaaacagatg gttctgcggg ccagacgttt gattcgggtt ctcagggtca gattgttacg
      241 ctcaatgtgc tgagtaaaaa tcttaccagt cagatgcttc tccttcggta actctctagc
      301 gtaactaccc cattcgtcag tggtcaacat gccgatggta aaagggtaag cagcgcccgc
      361 aattcacgac aggtttcgtc agtccggggg ccaaaagtgt aggccagaac accaccagtt
      421 ttcgtgttgt acacgtacca gagccagtgc tgtcgcgctt tactgccgac aaagctccat
      481 tgctgactaa gtttgcagat gagtgccaca tcagcatgag cgaccgggga tgacgttatt
      541 cttcgtggcg agagtttttt aaagttcgga tgactgtatt aatgccgatt ttcagcgtac
      601 gggcagtatc gcgaaccccg gcaccgttga aagtcatttc agcgatttgg tctttaactc
      661 ccggtttacg gggctcgtaa cagtaactga gctgaaatac ccggtgacag tcacggccgc
      721 ggaatcgatc atgtcttttt gggttctgcc cgtgacggta aacctgaacg gactggcaac
      781 gggggcaatg aaggttaact gtgaccatga aacaacctta aaagcgagta ttatatcctt
      841 aattcaacta attggaggca tcaccgttta ccggagcgac agcttgcgga aaggtcgtgc
      901 aactacaaag caacaaacag actacggcgg gaattatttt tttcatcatc cattaccacg
      961 aaaacagcgg ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg
     1021 gtgaaactac cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct
     1081 ggaccagcgg gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc
     1141 ccagcgcgtt ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc
     1201 atgagaagct gaaatcatac ggtttaccga gataacgttt tgccgtttgc gccagttttt
     1261 gctgttgttc aacactcagt ccgccttcaa cgcggcgaac gacgtatttg cccttttcac
     1321 catgggcgat ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt
     1381 agggcttttt gttgcgtatc accagcatac cggtgtggct gtaatcggaa tgggttgcca
     1441 gttggatcgc tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg
     1501 gctgccaggc aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca
     1561 tagttaaacg tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc
     1621 cggatgcggc gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa
     1681 tatgacgtag gcctgataag cgtagcgcat caggcggttc cactccgcgc cgctcttttc
     1741 cgctttagta ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg
     1801 cttccgggat ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc
     1861 cggtaaaggc atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt
     1921 tgttcacctg acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg
     1981 aaaccgtaat ctttttgact tgttcactct tcttgccgtg ctcagcgtat gggctgatat
     2041 attcgccgct ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag
     2101 accacacagt taatgtgggc actggccgta aacccgcata gtttaccgta caggcgctac
     2161 cgtgacatcg ggtaatccac ctgctggcgt gataatgcat ataattttaa cggctatttg
     2221 ggatttgctc aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattac
     2281 cacaatgttt actctggtgc ctgacatttc accaataaag cccagggaac atcatcacat
     2341 gacgcgtaaa caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg
     2401 ttgcgttgtc ccacccatcc atctttccag tacctataac tttaccggat ttaatgaacc
     2461 gcgcgcgcat gattactcgc gtcgtggcaa cccaacgcgc gatgtggttc agcgagcact
     2521 ggcagaactg gaaggcggtg ctggtgcagt acttactaat accggcatgt ccgcgattca
     2581 cctggtaacg accgtctttt tgaaacctgg cgatctgctg gtagcgccgc acgactgcta
     2641 cggcggtagc tatcgcctgt tcgacagcct ggcgaaacgc ggttgctatc gcgtgttgtt
     2701 tgttgatcaa ggcgatgaac aggcattacg ggcagcgctg gcagataaac ccaaactggt
     2761 actggtagaa agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca
     2821 tctggcaagg gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt
     2881 acaaaatccg ctggcattag gtgccgatct ggtgttgcat tcatgcacga aatatctgaa
     2941 cggtcactca gacgtggtgg ccggcgtggt gattgctaaa gacccggacg ttgtcactga
     3001 acttgcctgg tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct
     3061 gctacgcggg ttgcgaacgc tggtgccgcg catggagttg gcccagcgta acgcgcaggc
     3121 gattgtgaaa tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc
     3181 ggaaaatcag gggcatgaaa ttgccgcgcg ccagcaaaaa ggctatggcg caatgttgag
     3241 ttttgaactg gatggcgatg agcagacgct gcgtcgtttc ctgggtggac tgtcgttgtt
     3301 tacgctggcg gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac
     3361 acatgcaggc atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg
     3421 tatctccacc ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg
     3481 ggctgcaaac aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag
     3541 ctgcataaat ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc
     3601 attatggcgg agtactctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc
     3661 actaaccagt tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag
     3721 gttcaacaaa cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa
     3781 gaagccgata gcctcattag cgcttttgtc agcgaccttg agcgcctggc ggcgctgctc
     3841 gacagcggta ttaacgacgc agtgtatgcg gaagtggtgg ggcacggtga agtgtggtcg
     3901 gcacgcctga tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc
     3961 cgcgagtttt tacgcgctga acgcgccgca caaccgcagg ttgatgaagg gctttcttac
     4021 ccgttgctgc aacagctgct ggtgcaacat ccgggcaaac gtctggtggt gaccggattt
     4081 atcagccgca acaacgccgg tgaaacggtg ctgctggggc gtaacggttc cgactattcc
     4141 gcgacacaaa tcggtgcgct ggcgggtgtt tctcgcgtaa ccatctggag cgacgtcgcc
     4201 ggggtataca gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt
     4261 ctggatgagg ccagcgaact ggcgcgcctg gcggctcccg ttcttcacgc ccgtacttta
     4321 cagccggttt ctggcagcga aatcgacctg caactgcgct gtagctacac gccggatcaa
     4381 ggttccacgc gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc
     4441 cacgatgatg tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg
     4501 cataaagaga tcgaccaaat cctgaaacgc gcgcaggtac gcccgctggc ggttggcgta
     4561 cataacgatc gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg
     4621 aaaatcctcg acgaagcggg attacctggc gaactgcgcc tgcgtcaggg gctggcgctg
     4681 gtggcgatgg tcggtgcagg cgtcacccgt aacccgctgc attgccaccg cttctggcag
     4741 caactgaaag gccagccggt cgaatttacc tggcagtccg atgacggcat cagcctggtg
     4801 gcagtactgc gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc
     4861 cgcgcagaaa aacgcatcgg cctggtattg ttcggtaagg gcaatatcgg ttcccgttgg
     4921 ctggaactgt tcgcccgtga gcagagcacg ctttcggcac gtaccggctt tgagtttgtg
     4981 ctggcaggtg tggtggacag ccgccgcagc ctgttgagct atgacgggct ggacgccagc
     5041 cgcgcgttag ccttcttcaa cgatgaagcg gttgagcagg atgaagagtc gttgttcctg
     5101 tggatgcgcg cccatccgta tgatgattta gtggtgctgg acgttaccgc cagccagcag
     5161 cttgctgatc agtatcttga tttcgccagc cacggtttcc acgtcatcag cgccaacaaa
     5221 ctggcgggag ccagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc
     5281 gggcgtcact ggctgtacaa tgctaccgtc ggagcgggct tgccgatcaa ccataccgtg
     5341 cgcgatctga tcgacagcgg cgatactatt ttgtcgatca gtgggatctt ctccggcacg
     5401 ctctcctggc tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag
     5461 gcgtggcagc aagggttaac cgagcctgac ccgcgtgatg acctttccgg caaagacgta
     5521 atgcgcaagc tggtgattct ggcgcgtgaa gcaggttaca acatcgaacc ggatcaggta
     5581 cgcgtggagt cgctggtgcc tgcccattgc gaaggcggca gcatcgacca tttctttgaa
     5641 aatggcgatg aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg
     5701 ctggtgctgc gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag
     5761 gcggtgcgtg aagaccatcc gctggcatca ctgctgccgt gcgataacgt ctttgccatc
     5821 gaaagccgct ggtatcgcga caacccgctg gtgatccgtg ggcctggcgc ggggcgcgac
     5881 gtcaccgccg gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag
     5941 aaatttaata atgcccggta ctcatgtttt cgggtttatg gtttctaatg aaatatattg
     6001 aattatcata ggattaggcc ggattaagcg tttacgacga atccggcaag aagcaataag
     6061 tacatggtta gtttatattt gcagtccggt ttgctttgca taccggattt tctttttctt
     6121 accatcctga agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg
     6181 gttgacgccc ttcggctttt ccttcatctt tacatctgga cgtctaaacg gatagatgtg
     6241 cacaacacaa catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc
     6301 agccagcggg atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg
     6361 ttcgagtttt tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat
     6421 cgccttagca gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag
     6481 cgcgaccgta cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca
     6541 ccgcatctta cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac
     6601 tggaataacg gtattcgtca tatcgtggcg ctgcgtggcg atctgccgcc gggaagtggt
     6661 aagccggaaa tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat
     6721 atctccgtgg cggcgtatcc ggaggttcac ccggaagcaa aaagcgctca ggcggatttg
     6781 ctcaacctga aacgcaaagt cgatgccgga gccaaccgcg cgattactca gttcttcttt
     6841 gatgtcgaaa gctacctgcg ttttcgtgac cgctgcgtat cggcgggtat tgatgtggaa
     6901 attattccgg gaattttgcc ggtctcgaac tttaaacaag cgaagaaatt tgccgatatg
     6961 accaacgtgc gtattccggc gtggatggca caaatgtttg acgggctgga cgacgatgcc
     7021 gaaacccgca aactggtggg agcgaatatt gccatggata tggtgaagat tttaagccgt
     7081 gaaggagtga aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt
     7141 tgccatacgc tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac
     7201 agtgatcaca aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata
     7261 aggttatcag ccttgttttc tccctcatta cttgaaggat atgaacctaa aacccttttt
     7321 tataaagcat ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat
     7381 ctacatctct ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca
     7441 attataactt ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt
     7501 gatgagcacg tcagacgata ttcataacac cacagccact ggcaaatgcc cgttccatca
     7561 gggcggtcac gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca
     7621 actccgtgtt gacctgttaa accaacattc taatcgttct aacccactgg gtgaggactt
     7681 tgactaccgc aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc
     7741 cctgctgaca gaatctcagc cgtggtggcc tgccgactgg ggcagttacg ccggtctgtt
     7801 tattcgtatg gcctggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc
     7861 gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga
     7921 taaagcgcgt cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc
     7981 cgacctgttt atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg
     8041 ttttggtgcc ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga
     8101 aaaagcctgg ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac
     8161 cgagatgggc ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc
     8221 tgcggcagca gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt
     8281 ggcgctgatt gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa
     8341 cgtaggtcct gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac
     8401 ttacggcagc ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca
     8461 gacgccgacc cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca
     8521 gacccgcagt ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc
     8581 ggatccgttc gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct
     8641 gcgttttgat cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt
     8701 caacgaagcc tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc
     8761 tcgctacatc gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca
     8821 gccgatctac aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc
     8881 tggtctgtct gttagtgagc tggtatcggt ggcctgggct tctgcttcta ccttccgtgg
     8941 tggcgacaaa cgcggtggtg ccaacggtgc gcgtctggca ttaatgccgc agcgcgactg
     9001 ggatgtgaac gccgcagccg ttcgtgctct gcctgttctg gagaaaatcc agaaagagtc
     9061 tggtaaagcc tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc
     9121 cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg ccgggccgcg ttgatgcgcg
     9181 tcaggatcag actgacattg agatgttcga gctgctggag ccaattgctg acggtttccg
     9241 taactatcgc gctcgtctgg atgtttccac caccgagtca ctgctgatcg acaaagcaca
     9301 gcaactgacg ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg
     9361 tgccaacttc gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa
     9421 tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa
     9481 agagctgttc gaaggtcgtg accgtgaaac cggcgaagtg aaatacacag ccagccgtgc
     9541 ggatctggta tttggttcta actctgtcct gcgtgcggta gcggaagttt acgccagcag
     9601 cgatgcccac gagaagtttg ttaaagactt cgtggcggca tgggtgaaag tgatgaacct
     9661 cgaccgtttc gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct
     9721 gaagtttctt taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc
     9781 tgccgcagga aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg
     9841 gagttatagc tggattttca tgaagcaagt caccagctac atcggtgcct tcgactttac
     9901 cgccttacgc tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg
     9961 cggaatgcgc ccgacaccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg
    10021 gatggttggc ctggcgcagt gggcgctggt cagcggcgga gcggggaagg tggcgatcct
    10081 aagctatacc atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct
    10141 gcgacgtggg caatatttcg cgatactgat tgccgctttc ggcttatttt tggtgttgca
    10201 gccgtggcaa ctcgatttct cttcaatgaa aagtgccatg ctggcaatcc tctccggcgt
    10261 cagttggggg gcgagcgcga ttgttgctaa acgcctatac acccgtcatc cgcgcgtgga
    10321 tttattatcg ttaacatcct ggcagatgct ctatgcggcg ctggtgatga gtgtggtcgc
    10381 tttactggtg ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta
    10441 cagtgcgatt ctggcgacgg cgctggcgtg gagcttatgg ttgtttgtat tgaaaaactt
    10501 gcctgccagt attgccagct taagcacgct ggccgttccc gtttgcggcg tactcttttc
    10561 ctggtggctg ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt
    10621 gctggcactg gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg
    10681 aattttttct tcatgtgggg cgatcgctta tttaacaaaa taacgataat gccccaccat
    10741 ccgccagcta aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag
    10801 cggcgtaccg tcgcgggcga agccatctga caccacccca atatgtgcca gtccgttatc
    10861 cagtcgccag gagacaatat cgcccgcttg atagtcactg gggttcttgc tgatggggcg
    10921 tgttttatcg tggcgggtaa accaggtttc cagattaggc acccgacggt gatcgatgtt
    10981 gctgtccggg cgctttaact gccatttttg cgggtactcg gcaaaattct tcgccatatc
    11041 ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc aatgcacgga tcaccacatc
    11101 ggaacatacc ccgcgttctt gcgggacatc accgccagga taggtaagct gcatatacgc
    11161 cggatcgtaa aatagcgtgc tgccgatttg ctgtctggca ccgtctgcga tggcaaggtt
    11221 ggtattggcc tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct
    11281 ggtaaatgcc gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga
    11341 aaataagagt cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca
    11401 gaagcggata aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg
    11461 gatgcggagt gaacgcctta tccggcctac aaaagcacgc atattcaata tattgcagag
    11521 atttcgtagg cttgataaac gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa
    11581 gccgggagtt tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg
    11641 ctaccagcag agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtgaatgg
    11701 tttcaccttc tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt
    11761 taatatccag ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg
    11821 tttcgatttc ctcaaccggt gcgttttcca gaaccagctg cgtcagcgta ccaaatgcca
    11881 ctttttcacc gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg
    11941 cgtgagccgc cgccagcccg ccgctttcaa aaccaacgcc gctcaaatag gtgttcgctt
    12001 caatcacgcg ctccagcgcc ggggttacta catgctgttc ggcggcaagc atcgcttttt
    12061 cgccttcttc cagcagggtg ttgtagcaca gttcagccag tgccagcgcc gcctgggtgc
    12121 acttgccgcc cgccatggtg gtcgcgccgc tacgagagca agcacgcgct tcaaaccagg
    12181 ttgccagcgc atcgccgata cccgctgcta acagacgtgc aggtgcgccc gcgacgattt
    12241 tggtgtcgac aatgaccata ttcgggttat ttggcaacag cagatagcgg tcaaactcac
    12301 cctcatcggt gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg
    12361 gtgcgatcgc taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt
    12421 ttccgccacc gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac
    12481 ggtcgatctc attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag
    12541 catctttaaa gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca
    12601 ccactaacca gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag
    12661 cgccctggat gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga
    12721 tgagtagtgc caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc
    12781 gcaggggtgt tgagcatttg ttgcgctaca tctaagggca gggtgatgga ttcacatcct
    12841 gccagtaaac agtccagcgc ctgacgcggc gttttaaagc tggccgccag caccatgctt
    12901 tctggtgcgt gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg
    12961 ccctgggcat ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc
    13021 agtaaccctt gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc
    13081 agcatcttaa tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga
    13141 atggcgtcgc gcaggcgctt cgcttcttcc actataccct gcgcgtcacg actcatagtc
    13201 tgagcaaaca gaatgccctc atcaccaatc gctttttgca gacgcggcag aacttcccat
    13261 atggactcct tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat
    13321 atgcgtgcca gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg
    13381 tttcctttta cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg
    13441 cgttaaccag cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa
    13501 tgcgcatgtg ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg
    13561 cttcatcatc gccccagcgc accggcgctt gcagacgcgc cacgctgatg gtggattgct
    13621 caatgtgttc tgatttgctg tgtggaatgg caaaactaaa gcccagaccg gtagagaaaa
    13681 cggcctcgcg cgcccataag tcggcttcca gtttacgcgg atagcggcag cggcccgcca
    13741 gcagcaggtt atcggtcatg cctttgagca cttcttcttt gctgcgccag tcactttcca
    13801 gcgtgatgca ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca
    13861 gcaggtgttc cacttccagc gaagtacggc aggccattgc ctggttgagc aacttgcggc
    13921 actcacggct atcaagttgc gccatccgcg ctttcgccgc cggaattgat ggtgcgctca
    13981 tgctgagttc atccagccct aagccgacca gcaacggcag cacggaacct ttcgctccca
    14041 gctcaccgca cagaccaatc catttgccct ggcgatgcac cgcctgcacg gcgtaatcga
    14101 gcgcccgcaa gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcgttatcgc
    14161 gatccactgc cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct
    14221 cttcgcagca ttgatcgatg atgaacatca ccgacggtac ttccagcata atgccaagct
    14281 ggatcttctc atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt
    14341 tttctttcac ccataagatc tcttccattg aggagatcat cgggatcatg attttcaggc
    14401 tgccgtgagc ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt
    14461 cttcataaat acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca
    14521 gataatcaac gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt
    14581 tggcagactc cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg
    14641 tgcgatccat atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga
    14701 atgcggcctg cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt
    14761 cagcggtacg ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcgtcctgta
    14821 cgcgggcttc ttgctgataa taacgagcta cggcttctcc tggctcaacc acaatcgccc
    14881 cagcgttgcc gtcgatataa atcgtttgat gctgccacgg agtaagggca tcaatatcta
    14941 caccaaccag tgtcggaatg ttgaacgaac gggcaagaat tacggtatgt gaggtggtgc
    15001 cgccactttt cagtaacaga cctttgaggt gatttttatc cagttcgagg aactggctgg
    15061 gagtcagttc atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc
    15121 gttgctcacc gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac
    15181 gttcttgcag gtagctgctg ctggaacggg cgaactcttc gcaaaagtga ttcgcactgg
    15241 tgacaatcgc ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgcgca
    15301 gggaagtatc gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc
    15361 tatccagcag acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg
    15421 attgctcggc gtcaacgcct ttggctgcgg gaagattacc cagcgcattg agatctaaag
    15481 aagagatcgg cgtcagaatg ccgcctgcac taccgctgca cacggtgcgg gcgcggataa
    15541 tttgcggatt cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt
    15601 ccgccagcgg cgcatcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt
    15661 gcgcttcctg ttcgtcggcc ccggaaatca gtaactggca gttatcgccc gccagcgtat
    15721 cggtgccaat cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat
    15781 gccactcaat ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg
    15841 catgtacgcc gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc
    15901 cggtacggtt ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat
    15961 ctgccaaaag ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc
    16021 tgttttgcgg catgcttccg gtttatcgca agttatgagg cggatcgcat ttttgtactg
    16081 atattacaaa agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc
    16141 tattgtttcc tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg
    16201 aatgagttgg tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac
    16261 atgattccct tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc
    16321 aaaggcgcag tgccggatgc cgtagccgat ccaaatctga aaaaactgtt tgatatcggc
    16381 gttgcgggct tgacgctgat ggtgcctttc ctcgcagcgt acatcggtta ttccattgca
    16441 gagcgttctg cgctggctcc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg
    16501 ggcttctttg gtgcactgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag
    16561 aaaattccgg tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc
    16621 ggcacactga ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg
    16681 accaacagcc tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg
    16741 gtgatcatgg gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat
    16801 gccttcatgc tgatttgcgt tgctcagggt gtttataccg tggtggccat cgctgccgtg
    16861 ggtatttgta tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc
    16921 tccgcagaag agcgcgaaac cggcaaagcg gcactggtga tgggctgtgt tggcgttact
    16981 gaaggggcga ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc
    17041 ggttcagttt gtggtgcagt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg
    17101 ggtggtctga ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct
    17161 gtcggagcag tggtgacggc tgtttgtgtg aacgtgctga aaagtctggc gcgtaaaaat
    17221 gggtcttcga ctgatgaaaa agaagacgac ctggatttgg attttgaaat taattaattg
    17281 aggaacgagc catgacgaaa attattgcag taaccgcatg tccttcaggt gttgcccata
    17341 cctatatggc agcagaggcg ctggaaagtg ccgcgaaagc gaaaggctgg gaagtgaaag
    17401 tagaaaccca ggggtcaatt ggtctggaaa atgaactgac tgcggaagat gtggcgagcg
    17461 ctgatatggt gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca
    17521 agaccatcgt gcgcgtcaac atcagcgatg cagtaaaacg tgctgacgcc attatgagca
    17581 aaattgaagc tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt
    17641 cgctttccca caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt
    17701 tgccaatacc cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca
    17761 aaccgaaggc gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca
    17821 tgttgaaatt tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg
    17881 cgctgggatt atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt
    17941 cccgacgcgt ccgcaggacc gctttgctat cagtgaagaa gataaacgta tctaccgtga
    18001 agagttgttc ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac
    18061 agatgaagta aaagccgcga ccagcacgca gattttcagc atcaaccaga cagataaagg
    18121 ccaggggcac attattattg attacccacg cctgctgaat cacgggctgg gggagctggt
    18181 ggcgcagatg cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct
    18241 gttactgctg gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat
    18301 ggcggcaaac tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc
    18361 ccgccataac gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta
    18421 catgaacatc attctgcaat acgaatccaa cgccagttcg ctatcgttgg ggcgcttcga
    18481 ccagtatatg ttgccgttct atcaggcatc tttaacccag ggcgaagatc cggcgttcct
    18541 gaaagaacta ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac
    18601 cagcagcgcg cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt
    18661 aaccgagaac ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcttatca
    18721 aagcgtgcaa ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc
    18781 gttcctgatg aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa
    18841 cgatgaagtg gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga
    18901 ctattccgta gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga
    18961 catcgcgatg tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa
    19021 tgctgcgctg acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat
    19081 caccctgatg gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt
    19141 accgctgctc tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg
    19201 cggcgcgcgt tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc
    19261 tctccatgcg ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct
    19321 gtcggtatta aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat
    19381 taaccgcttc gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact
    19441 gttacgccac tactgcaaag aagtggaaaa ataccagaac ccgcgcggcg gttacttcac
    19501 gccgggatcg tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc
    19561 agacggtcgt tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca
    19621 ggacgcacaa gggccaacag cggtactgaa gtcagtcagt aagctcgata acacgctgct
    19681 gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg
    19741 attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca
    19801 gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc
    19861 cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat
    19921 ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgcg
    19981 atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc
    20041 gtacggtggt cttttttaaa ggctgtccgc atctttgccc gtggtgtgct aatccggagt
    20101 cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat
    20161 gtttgcgtga tgcggatgaa tgcccctccg gtgcgtttga acggattggt cgcgatatca
    20221 gccttgacgc tctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg
    20281 gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc cgagtttgct acccgttttt
    20341 tacagcgact gcggctgtgg ggcgtgtcat gcgccatcga aactgccgga gacgcgccag
    20401 ccagcaagct gttaccgctg gcgaaattgt gcgatgaagt gttgttcgat ttaaaaatta
    20461 tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc
    20521 tgcgtttgct ggtgagtgaa ggtgtcaacg tgatcccgcg tttgccgctg atccccggtt
    20581 tcacactcag ccgggagaat atgcagcagg cgctggacgt gctgatcccg ctgaatatca
    20641 ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgt ctgctgggga
    20701 aaacatggtc gatgaaagag gtttctgcgc cgtcgtcagc cgatgtggca acgatgcgcg
    20761 aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg
    20821 gcagtaaccg cctgcgtcag tggcgtggcg catacttata tggcggcgga acggctggaa
    20881 aagttgtgcc agttagagaa gtggggcgtc agcattgaaa ctcagggcgc gctgggaacg
    20941 gagaatcgtt tagcggacga ggatatccgc cgggcggatg ttgcgctgtt gattacggat
    21001 atcgagcttg ctggtgccga gcgatttgaa cattgtcgct acgtgcaatg cagtatctac
    21061 gcgttcctgc gtgagccgca gcgggtgatg agcgcggtac gtaaagtgct ttctgcgccg
    21121 cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc
    21181 gacgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc
    21241 tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata
    21301 acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg
    21361 tcttttgaaa caggtgcgag agatagtttg gtgaaatata gaacgcctgt gcgacagatt
    21421 cgcgggtaag cgcggaggca tagcgttcgt cgatataatc acgaatagct tcgaatagag
    21481 cctggctgcg tgaggcggtc tgtatttggc tgccgagcaa atcgcggcag tggctaagca
    21541 ggctggcgac gataagcctt gccgtttgct gctcctgtgg ctgcatttgc atttcattga
    21601 gcgtttgtag cagaaaagaa ccaatgcgcg ggccgcgtcg ggcgacgtgt tgcttcgcca
    21661 gattttgata ctgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa
    21721 acagcacgct aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcgg
    21781 gaacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac
    21841 cttccagcac tatttccagc cgtggaaaat cgacctgata cgcaagatcg ggcgccggac
    21901 cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga
    21961 gcagatagct gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta
    22021 actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac
    22081 attgcggcag tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga
    22141 ttacccacct gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac
    22201 gggttaccaa tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga
    22261 tcgtaaccgt cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg
    22321 ctgtatttac gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc
    22381 gacgtccaca gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc
    22441 ccctgagttt tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc
    22501 aggaaaccgt tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg
    22561 ttatacaggt tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca
    22621 ggcggaacat gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg
    22681 tatttgatat gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc
    22741 agcacttcct ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt
    22801 tgctggttca tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg
    22861 cgggcggtca tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc
    22921 atgttcatca gcgacggctg cgtcagatac agatccgggt tcttttcatt ggcaaaggtc
    22981 agcgcctgtt gcaggatttc aatggtgtac ggacgagacg taactacgtt attaaacacg
    23041 gtcagattcg gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa
    23101 ccgtacagac tcatgcgtcc gcgctgggtc gactcgccaa tcaccagcac taaagtgcgc
    23161 ggttcgttac ccgattcatc tttgaaatta gccagtggcg gcaaggcatt attttcattc
    23221 agtaactttg ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc
    23281 cacggtgcgg caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg
    23341 tttttgataa acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa
    23401 gagacaacat aacgccacgg ctttggaata tagaccgggc gcaggcgtgt ccacagcaga
    23461 actgccaccg ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt
    23521 aaatactcgc tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag
    23581 aactcctgcc cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc
    23641 acgccgatta ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac
    23701 cacagcgagc tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg
    23761 taaataatgg cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg
    23821 gctttccagc taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt
    23881 cttgaaaaaa atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc
    23941 agaatttgaa taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta
    24001 tttcagaaaa ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat
    24061 tacgcatacc tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat
    24121 ccggttcctg gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata
    24181 cgttcagcgg gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat
    24241 cggccattag atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca
    24301 ggttgcgtaa ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt
    24361 attccgccag ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg
    24421 agaagaatgg ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca
    24481 ctttttgcag cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc
    24541 aggcaaaaat ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg
    24601 ccggacgtga acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt
    24661 aaggcacaaa atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg
    24721 acagttcatc cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg
    24781 cttccagaat cgccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat
    24841 atttaaagcg gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg
    24901 gcggttgtga cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc
    24961 gaccgtggaa caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct
    25021 gtgcctgata ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat
    25081 agccaatcat caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca
    25141 gctgggtcat gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa
    25201 ccggcatcgc aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg
    25261 acggcgtttt cgccatcgag atcacgtagg cggcaatgga gccttgcggc gcttcggcaa
    25321 tcacctggca ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca
    25381 gaagcggacg tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc
    25441 agctttcgta gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatggc
    25501 gggtgctctc ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc
    25561 gcagggtgtc gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact
    25621 ggtagcaagc gtagagcggt tcccacagtt cttcgttttg tgtcagcagc ccttctggtt
    25681 ttggcagttc ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag
    25741 aacgcaggtt tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg
    25801 ccagcagttc aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt
    25861 tcaggaacaa atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg
    25921 cagtgacgtt cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa
    25981 caaattcgac gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca
    26041 ggtaatttgg tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt
    26101 catctaccgg gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta
    26161 actggcgcag gcgacgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga
    26221 gctgttttaa gcaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt
    26281 cggtagggtg agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg
    26341 tgtcttcgct cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg
    26401 ggttgctggc agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca
    26461 ggttcaggaa ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt
    26521 gtaaggtggt gagtaactcc tggcggttag catcattgcc agcgcgtgaa gatttggaca
    26581 acttacggat agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt
    26641 ctcccagcac tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca
    26701 tattacccca gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg
    26761 cgcgaatatg ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga
    26821 cgtcaaatgc tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgtcattaa
    26881 attcacgacg ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc cccaagtaac
    26941 accaaaggtg taggtcggat aagatgcgca agtatcgcat ccgacattat tgcggcactg
    27001 gagtttgaca acagtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc
    27061 agcaaaaatg gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt
    27121 ccagatattc atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg
    27181 ggcataacgt ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc
    27241 cgagcaattt ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga
    27301 tcggcggatg cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca
    27361 atgcatcgtt gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat
    27421 ccatatgcaa ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc
    27481 cgaggttgag cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt
    27541 tatcgcgcaa ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc
    27601 cgcgtgctgg atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat
    27661 gacctttatg tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg
    27721 ggcgcagggc ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag
    27781 cagtcgccag aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat
    27841 caaggataaa cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt
    27901 catgctccgt cagtgtaaac ggatcacgcg tccagcgacc gtcatcaaat ggtaccgtat
    27961 cggtatgccc cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattaa
    28021 atttgttgcg agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc
    28081 agtccgccag cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc
    28141 ttattgaagg tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt
    28201 tcattgttga cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa
    28261 aaatacacta acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt
    28321 aacctggcaa ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg
    28381 tgccagcggc tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa
    28441 cataaccgct ttgactgttt cagcgcaaag caatgatgcg ggcaagttaa tctccgattt
    28501 gcatccgcag ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga
    28561 gtttagccca ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt
    28621 agcgccgcag tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt
    28681 taacgacgcc accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt
    28741 ggaacaggca gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt
    28801 gattgcggtg ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga
    28861 tgccgatctt cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg
    28921 tgcagggcgt aaagcggcca tttcaaacag cttttgtgaa gttagcctgc aaccgtatgg
    28981 cgtctttact catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt
    29041 caccccacat ctgggcaatt tcccgcgcgg cattctcgaa accattacct gccgcctgaa
    29101 atcgggtgtg acccaggcgc aagtcgcgca agtgttacag caggcgtatg cccataaacc
    29161 gctggtgcgg ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatt
    29221 ttgcgatatc gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa
    29281 cttactgaaa ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc
    29341 ggaaacgcag tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg
    29401 gcgtactgct ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc
    29461 gtgagtcaca tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc
    29521 tgatgaaagg gctgaatctg ccggtgaaaa agaaaaacgg cctgcgggtg acgcctgctg
    29581 atcagataga cattatcacc ggagcactgg cgggaacggc aaataaaacc ctgttggcat
    29641 gggcgaagaa acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca
    29701 aagtgaccca gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta
    29761 agcttatcaa ctccttgctg gagaacggtt atctgccggt ggtcagctcc attggcgtaa
    29821 cagacgaagg gcaactgatg aacgtcaatg ccgaccaggc ggcaacggcg ctggcggcaa
    29881 cgctgggcgc ggatctgatt ttgctctccg acgtcagcgg cattctcgac ggcaaagggc
    29941 aacgcattgc cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta
    30001 ctgacggcat gatagtgaaa gtgaacgcgg cgctggatgc ggcccgcacg ctgggccgtc
    30061 cggtagatat cgcctcctgg cgtcatgcgg agcagcttcc ggcactgttt aacggtatgc
    30121 cgatgggtac gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt
    30181 gaatttcaaa ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag
    30241 cagatcaacg gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc
    30301 aggatattgt tggctctgtg gcctggtcca aagccctggt cacggtaggc gtgttaaccg
    30361 cagaagagca ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca
    30421 ggccacaaca aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac
    30481 tgatcgacaa agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc
    30541 aggtagcgac tgacctgaaa ctgtggtgca aagataccgt tagcgagtta ctgacggcta
    30601 accggcagct gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtaatgc
    30661 caggttacac tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct
    30721 atgttgagat gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg
    30781 tcagcccgct aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt
    30841 tagcaggctg gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc
    30901 gtgaccatgt gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt
    30961 ttgctgaaga tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc
    31021 gcgtgacttc cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga
    31081 ttcgcggtaa atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag
    31141 gtttgccgct ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc
    31201 tcgatacctg gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga
    31261 aacgtccacg ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg
    31321 attatctggt ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg
    31381 tggtggaagc cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga
    31441 aattcagccc ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg
    31501 acaagcgtgc ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg
    31561 cgcaggcgcg gttagagtaa tttgtttata ttgctcccct gaaaatgggg agcttaccac
    31621 gcctacaaag tgcgacactt cgtaggcgga taaggcgttt acgccacatc aggcagaaat
    31681 ttgtcagcat tctggtgagc ttaaatttaa cgcccaacca gaaccattat ttgtcacatt
    31741 cctttttatc attgtgtctt ctttaccttc acactcccca tgaaaatcat attttcaaat
    31801 acatcaacca atacatattt taattaattg aatataatta ttttctattg ctgtgcccat
    31861 ttttaatgat taatggagtt tgttatgtga ttatttaacg tgttgtttta acgataatgg
    31921 ttaattattc aggtggtttt tgagggtatt ttttctcctc ggtggaaatt tttgttttta
    31981 atgtgatcac ggttgaataa tctctttttc tctcttgtat ggtagtaggc gtaaaattaa
    32041 gtttgaaacg tctatttaca acacaggaga ttgcaataat gaaaattatt gctgcagatg
    32101 tttttgttac ttgcccaggg cgtaatttcg ttacgcttaa aataaccact gaaagcggac
    32161 tttgtggatt aggagatgcc acgttaaatg gtcgcgaatt gtcggtcgct tcttatctga
    32221 aagatcatct ttgtccacaa ctaattggcc gcgatgccag ccgtatcgaa gatatctggc
    32281 aattctttta taaaggtgct tactggcgac gtggccccgt cacgatgtca gccatttctg
    32341 ccattgatat ggcgttgtgg gatatcaaag ctaaagcggc aaatatgcca ttgtatcaac
    32401 tgcttggtgg tgcttctcgc gaaggcgtga tggtttattg ccacaccacc gggcggacga
    32461 ttgatgaagt ccttgaagac tatgcaaagc accagcagat gggctttaag gcgattcgtg
    32521 tgcagtgcgg tgtaccgggt atgcaaacta cttatggcct ggcgaagggc aaaggcctgg
    32581 cgtatgaacc ggcaaccaaa ggcctgtggc cagaagagca gttgtggtct tcagaaaagt
    32641 atctcgattt tacgccgaag ttatttgaag cggtgcgtaa taagttcggt tttaacgaac
    32701 atctgttgca tgacatgcac catcgcctga cccccatcga agcggcgcgc tttgggaaga
    32761 gcattgagga ttaccgcctg ttctggatgg aagatccgac gcctgcagag aaccaggaat
    32821 gttttcgtct gattcgccag cataccgtga cgccgattgc ggtcggtgaa gtgtttaaca
    32881 gtatctggga ctgcaagcag ttaatcgaag agcagctgat tgactatatt cgcacgacga
    32941 tcacccatgc aggcggcatt accgggatgc ggcggattgc cgattttgca tcgctttatc
    33001 aggtacgtac cggttcgcat ggcccttcag atttatcgcc tgtttgtcat gcggcggcgc
    33061 tgcattttga cctatgggta ccaaattttg gcgttcagga gtacatgggc tattccgaac
    33121 aaatgcttga agttttcccg cacagctggc gcttcgagga aggttatatg cacccaggcg
    33181 atgaaccggg gctgggtatt tctttcgatg agaaactggc ggcgaaatat ccgtatgacc
    33241 cggcgtatct ccccgtcgca cgactggaag atggcacatt gtggaactgg taataacggt
    33301 tcgcttaatt attttctgaa tagtcatcaa atcgattaat tccgctcaag gagctaaaaa
    33361 tggacgttga tgtgtcaacc agtgttgcgg gtaataaacc gcaacggatt cgtcgtattc
    33421 agacagtgac cctggtttta ttatttatgg cggggatcgt taattttctc gaccgttcgt
    33481 cattgagcgt ggcaggtgaa gcgattcgtg gcgagttagg attatcggcg acagagtttg
    33541 gtgttttgct ttctgcattt tctctgtctt atggtttttc acaactacct tccggtattt
    33601 tgctagaccg ctttggccca cgaattgttt taggcgcagg cttaatattc tggtcattaa
    33661 tgcaggcatt aacaggaatg gttaattctt ttagccactt tattttaatg cgtatcggtc
    33721 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
    33781 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
    33841 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
    33901 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
    33961 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgccg gttaaaccgc
    34021 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
    34081 tgattttagg tttctctggc gtcaattata ccggttggct ctacatcgcg tggttacccg
    34141 gttatttgca agccgagcaa ggtttcagtc tggcgaaaac cggttgggtg gcggcgagtc
    34201 ctttcctcgc ggcggcagtc gggatgtggg ttaacggtat tgttgtcgat cgactggcga
    34261 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
    34321 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agcccaggcg gtagcgttta
    34381 tctcaatggc gctattctgc gtgcatttcg ctggaacgtc tgcatggggg ctggtgcagg
    34441 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
    34501 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
    34561 atctggcgtt ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
    34621 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
    34681 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
    34741 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
    34801 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
    34861 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
    34921 ggtggcattg gccgagcacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
    34981 gccgacgctt agcgggcaaa ttcgtaagct ggaagatgag ttgggcgtga tgttgctgga
    35041 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtag atcaggcgcg
    35101 taccgtactg cgtgaggtga aagtccttaa agagatggca agccagcagg gcgagacgat
    35161 gtccgggccg ctgcacattg gtttgattcc cacagttgga ccgtacctgc taccgcatat
    35221 tatccctatg ctgcaccaga cctttccaaa gctggaaatg tatctgcatg aagcacagac
    35281 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
    35341 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
    35401 ctatgaagat cacccgtggg cgaaccgcga atgcgtaccg atggccgatc tggcagggga
    35461 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgttt
    35521 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaga cgctgcgcaa
    35581 catggtggcg gcaggtagcg ggatcacttt actgccagcg ctggctgtgc cgccggagcg
    35641 caaacgcgat ggggttgttt atctgccgtg cattaagccg gaaccacgcc gcactattgg
    35701 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
    35761 ccgagcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
    35821 acgcagctac ccgataggct tccgccatgg tcgggtagtt aaaggtggtg ttgacgaagt
    35881 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
    35941 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
    36001 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc gcgtgccaga tgtttaaact
    36061 gggcgcggcc cacttcatat ggcactttca tcgcggtcag ctgctgttcg gttttgccca
    36121 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgcgcgg
    36181 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
    36241 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
    36301 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
    36361 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
    36421 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gccttcgatc ttctcgtact
    36481 cttcgttgtg acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca
    36541 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
    36601 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
    36661 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
    36721 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
    36781 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
    36841 agcgagcgtt tccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
    36901 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
    36961 gcagtcggga atggtcgctg taaagtgggt tttgattgaa ttctataatg cggctgacgg
    37021 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
    37081 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
    37141 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
    37201 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattttatcg cgggtaagcc
    37261 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
    37321 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
    37381 tttccgctgt acgtaaaaga accggccaaa gaattgcagt aaatatgttt tattgcgtta
    37441 ccgttcattc acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag
    37501 tacttttggc aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac
    37561 ccgccgttcg ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag
    37621 cctgagtttg cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca
    37681 tttccgcgac gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg
    37741 ccaactcatg cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgcacctc
    37801 ggtctccaca tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg
    37861 ggaacgctcc ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt
    37921 cattgcggaa cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga
    37981 agcgcaagcc gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt
    38041 cggcgtcgaa caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc
    38101 gaaaggggct tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa
    38161 tgtgaaggac gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg
    38221 gttgctggct tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt
    38281 tctgtattcc cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt
    38341 aatcgcacca tgccggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta
    38401 ctgctgtaca gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg
    38461 gcagtactct ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag
    38521 gaagttgctg agtaatcggt attatgccgg atgctggcgc atccggcatg ggttttactt
    38581 cgcggtcagt aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc
    38641 cagacgttcg accttgtgcg tctggcttaa tgtttccaga ttcttgcata acgtttccgg
    38701 attgcaggag atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc
    38761 cagaccgctg cgcggtgggt cgacaaaaat ggtttcgcac tgataactct tcaagtctat
    38821 cccttgcagg cgattaaact cgcgtacacc attcatcgcc tgagtaaatt cttctgccgc
    38881 catacgaata atttgtacgt tatcaatatg gttagctgcg atgttgtatt gcgcagcagc
    38941 aaccgacggc ttagcgattt cggtggctaa tacccgatca aaattgcgcg ccagtgctaa
    39001 tgaaaagtta ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac
    39061 gtccagcgcc cattccagca tctgaatatt catcgccgcg ttcggctggg taaaactgtt
    39121 ttctacctga cggtagatca tctctttccc tgcgaccggc agacgttcat cgatgtaatc
    39181 ttgatccagc tcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg
    39241 cgcgcgcagt gcatcgcgca gggcttccgc ttcctgacgc cactcatcat ccagcttttt
    39301 atggtacaac agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa
    39361 caacttgtgg cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg
    39421 gttgatgagt tcactggcag cggggaagct atccacgcgg atgcggcttt tggtttgttg
    39481 atcgaaaatg atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcacgcat
    39541 ccggtaatga ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat
    39601 catactttgc aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag
    39661 gtgttcgggg gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc
    39721 tcatcagatg tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt
    39781 gttacaacct gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat
    39841 ctggagctga cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca
    39901 tccggtggga agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc
    39961 cggccaacgt cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct
    40021 tcttctattg tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttctg
    40081 tcacagcctt ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta
    40141 accgttttga acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg
    40201 atatcgaccg ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg
    40261 atatcaccca aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg
    40321 ccagtcatgt gttggtgtta attgatggcg tacgcctgaa tctggcgggg tgagtggttc
    40381 tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc
    40441 gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg
    40501 cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga
    40561 tgtctctacg cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc
    40621 ccatactcat ggttatgatg ttgttgccta tggtaatacc ggaacgcaag cgcagacaga
    40681 taacgatggt tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc
    40741 ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta
    40801 ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg
    40861 gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa
    40921 agattacaac tacgatcccc attttggtcg ttatgattcg tcggcgacgc tcgatgagat
    40981 gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc
    41041 gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg agaatggata
    41101 tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg attttacctt
    41161 tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac
    41221 cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta
    41281 taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga
    41341 gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat
    41401 ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata
    41461 ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg
    41521 accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac
    41581 gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga
    41641 cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc
    41701 ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta
    41761 tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta
    41821 tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac
    41881 cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct
    41941 atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg
    42001 cttttccgta tggcgaaaaa agcgaagagt ttattgttga gcgagtggtg gcaattgtca
    42061 ccgcagtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac accgccagta
    42121 ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg
    42181 cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg
    42241 gaactgttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga
    42301 tagaaatgct gggctcggca gagatggttg agttggctga agcgaagcta catggcgaag
    42361 atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc
    42421 cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa ctcttacagg
    42481 tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc agaacggcct
    42541 ggctgttaga acatgaagct ccggatgcaa aatctgccga tgcgaatatt gccttttgta
    42601 tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa
    42661 cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta
    42721 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    42781 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaactccg acagagaaag
    42841 caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc gcgccgctga
    42901 gaaaaagcga agcggaactg ctctttaaca atttatcag
//
LOCUS       DAZGCS010000032        42609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGCS010000032 DAZGCS010000000
VERSION     DAZGCS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.032"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..329)
                     /gene="yaaX"
                     /locus_tag="ACHK01_001799"
     CDS             complement(33..329)
                     /gene="yaaX"
                     /locus_tag="ACHK01_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO8096763.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(543..1829)
                     /gene="thrC"
                     /locus_tag="ACHK01_001800"
     CDS             complement(543..1829)
                     /gene="thrC"
                     /locus_tag="ACHK01_001800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO8096764.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1830..2762)
                     /gene="thrB"
                     /locus_tag="ACHK01_001801"
     CDS             complement(1830..2762)
                     /gene="thrB"
                     /locus_tag="ACHK01_001801"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO8096765.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2764..5226)
                     /gene="thrA"
                     /locus_tag="ACHK01_001802"
     CDS             complement(2764..5226)
                     /gene="thrA"
                     /locus_tag="ACHK01_001802"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO8096766.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5252..5371)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5307..5372)
                     /gene="thrL"
                     /locus_tag="ACHK01_001803"
     CDS             complement(5307..5372)
                     /gene="thrL"
                     /locus_tag="ACHK01_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO8096767.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5586..6272)
                     /gene="yjtD"
                     /locus_tag="ACHK01_001804"
     CDS             complement(5586..6272)
                     /gene="yjtD"
                     /locus_tag="ACHK01_001804"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8096768.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6672..6812)
                     /gene="yjjY"
                     /locus_tag="ACHK01_001805"
     CDS             complement(6672..6812)
                     /gene="yjjY"
                     /locus_tag="ACHK01_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO8096769.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6908..7624
                     /gene="arcA"
                     /locus_tag="ACHK01_001806"
     CDS             6908..7624
                     /gene="arcA"
                     /locus_tag="ACHK01_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO8096770.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHK01_001807"
     CDS             complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHK01_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO8096771.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHK01_001808"
     CDS             complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHK01_001808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO8096772.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHK01_001809"
     CDS             complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHK01_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO8096773.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHK01_001810"
     CDS             complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHK01_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO8096774.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11904..12773
                     /gene="robA"
                     /locus_tag="ACHK01_001811"
     CDS             11904..12773
                     /gene="robA"
                     /locus_tag="ACHK01_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO8096775.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHK01_001812"
     CDS             complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHK01_001812"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO8096776.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHK01_001813"
     CDS             13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHK01_001813"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO8096777.1"
                     /translation="MHQVVCATTNPAKIQSILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHK01_001814"
     CDS             complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHK01_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO8096778.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHK01_001815"
     CDS             complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHK01_001815"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO8096779.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16639..18306
                     /gene="ettA"
                     /locus_tag="ACHK01_001816"
     CDS             16639..18306
                     /gene="ettA"
                     /locus_tag="ACHK01_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO8096780.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHK01_001817"
     CDS             complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHK01_001817"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO8096781.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHK01_001818"
     CDS             complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHK01_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO8096782.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHK01_001819"
     CDS             complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHK01_001819"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO8096783.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLHS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHK01_001820"
     CDS             22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHK01_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO8096784.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23043..24059
                     /gene="lplA"
                     /locus_tag="ACHK01_001821"
     CDS             23043..24059
                     /gene="lplA"
                     /locus_tag="ACHK01_001821"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO8096785.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24091..24354
                     /locus_tag="ACHK01_001822"
                     /pseudo
     CDS             24091..24354
                     /locus_tag="ACHK01_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHK01_001823"
     CDS             complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHK01_001823"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO8096786.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHK01_001824"
     CDS             complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHK01_001824"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8096787.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHK01_001825"
     CDS             complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHK01_001825"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO8096788.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHK01_001826"
     CDS             complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHK01_001826"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO8096789.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHK01_001827"
     CDS             29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHK01_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO8096790.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHK01_001828"
     CDS             30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHK01_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO8096791.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31875..32657)
                     /gene="yjjV"
                     /locus_tag="ACHK01_001829"
     CDS             complement(31875..32657)
                     /gene="yjjV"
                     /locus_tag="ACHK01_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8096792.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(32654..33727)
                     /gene="yjjU"
                     /locus_tag="ACHK01_001830"
     CDS             complement(32654..33727)
                     /gene="yjjU"
                     /locus_tag="ACHK01_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO8096793.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(33849..34010)
                     /gene="ytjA"
                     /locus_tag="ACHK01_001831"
     CDS             complement(33849..34010)
                     /gene="ytjA"
                     /locus_tag="ACHK01_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO8096794.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(34137..34742)
                     /gene="osmY"
                     /locus_tag="ACHK01_001832"
     CDS             complement(34137..34742)
                     /gene="osmY"
                     /locus_tag="ACHK01_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO8096795.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(35135..36724)
                     /gene="prfC"
                     /locus_tag="ACHK01_001833"
     CDS             complement(35135..36724)
                     /gene="prfC"
                     /locus_tag="ACHK01_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO8096796.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(36815..37492)
                     /gene="yjjG"
                     /locus_tag="ACHK01_001834"
     CDS             complement(36815..37492)
                     /gene="yjjG"
                     /locus_tag="ACHK01_001834"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO8096797.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(37507..37953)
                     /gene="rimI"
                     /locus_tag="ACHK01_001835"
     CDS             complement(37507..37953)
                     /gene="rimI"
                     /locus_tag="ACHK01_001835"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8096798.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(37922..38335)
                     /gene="holD"
                     /locus_tag="ACHK01_001836"
     CDS             complement(37922..38335)
                     /gene="holD"
                     /locus_tag="ACHK01_001836"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO8096799.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            38438..39469
                     /gene="rsmC"
                     /locus_tag="ACHK01_001837"
     CDS             38438..39469
                     /gene="rsmC"
                     /locus_tag="ACHK01_001837"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO8096800.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            39737..39823
                     /locus_tag="ACHK01_001838"
     tRNA            39737..39823
                     /locus_tag="ACHK01_001838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39771..39773,aa:Leu,seq:cag)
     gene            complement(39862..40098)
                     /gene="yjjZ"
                     /locus_tag="ACHK01_001839"
     CDS             complement(39862..40098)
                     /gene="yjjZ"
                     /locus_tag="ACHK01_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO8096801.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            40239..41027
                     /gene="fhuF"
                     /locus_tag="ACHK01_001840"
     CDS             40239..41027
                     /gene="fhuF"
                     /locus_tag="ACHK01_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO8096802.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(41065..41742)
                     /gene="bglJ"
                     /locus_tag="ACHK01_001841"
     CDS             complement(41065..41742)
                     /gene="bglJ"
                     /locus_tag="ACHK01_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO8096803.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFTAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(41700..42425)
                     /gene="yjjQ"
                     /locus_tag="ACHK01_001842"
     CDS             complement(41700..42425)
                     /gene="yjjQ"
                     /locus_tag="ACHK01_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8096804.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10156 a        11624 c        10703 g        10126 t
ORIGIN      
        1 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
       61 ccgccgtgat gatcatgatg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
      121 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
      181 tgaccgccat cccagtaata accgcgatta tcacgatcgc ctatctgtaa ttttactgac
      241 gggactaacg taatttccgc agcctgcgct gccatgggag cgaccagaac cagggaaagt
      301 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
      361 gtcgatatcc ggagattacg cgatgagaac ggcggttgtt attctccgca atcctaatct
      421 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
      481 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
      541 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
      601 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
      661 agaatcgatt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
      721 tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc
      781 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
      841 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
      901 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
      961 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
     1021 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
     1081 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgtgg cagctgcgca
     1141 acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagct
     1201 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
     1261 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
     1321 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
     1381 ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
     1441 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
     1501 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
     1561 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
     1621 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
     1681 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
     1741 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
     1801 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
     1861 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
     1921 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
     1981 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
     2041 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
     2101 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
     2161 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc cgtcgagact
     2221 ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
     2281 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
     2341 tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc cagcaaacga
     2401 gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
     2461 caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt catcgccact
     2521 ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
     2581 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
     2641 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt caccgccgcc
     2701 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
     2761 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
     2821 tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
     2881 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc
     2941 cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa catagcgcaa
     3001 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
     3061 cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg gcagcacagg
     3121 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaatcaatag
     3181 cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat
     3241 ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
     3301 ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag
     3361 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
     3421 tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca tcgacgaggt
     3481 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt cggcatattg
     3541 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
     3601 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc
     3661 ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg agttggcaac
     3721 accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac gcttcagttg
     3781 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
     3841 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
     3901 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
     3961 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
     4021 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
     4081 cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgtgg
     4141 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
     4201 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
     4261 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
     4321 gtcttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
     4381 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
     4441 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
     4501 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
     4561 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
     4621 aagtaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca acaccatgtg
     4681 atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
     4741 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
     4801 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
     4861 cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat gcaggacatg
     4921 ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg ggaaccccgg
     4981 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
     5041 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
     5101 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
     5161 atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
     5221 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
     5281 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg
     5341 gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc
     5401 tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt
     5461 aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt tttttaatcc
     5521 acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc
     5581 gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa cattgccgtg
     5641 tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt cgaccagttt tatgtcatct
     5701 gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg cagttgatgt
     5761 tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa ttgatagcaa
     5821 tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat cggcacacca
     5881 gtaagaacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc gcgaccaaac
     5941 accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac cagttctact
     6001 ggcgtggcgt agtaatgata tttcgcccga ctgcgcgcag tggtggcgac agtgaaatcg
     6061 acatcgtgta acgattcaac cagtgtcggg aaaactttaa tattatcaat aatatcacca
     6121 gatccatgtg cgacccagcg ggtggctggc tccagatgtg cctgactatc gacaatccgc
     6181 agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt ttctgctctg
     6241 gcgggtgcga ccagaataat cgttatacgc atattgccac tcttcttgac caaataaccg
     6301 cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt cttaacgtaa
     6361 gtcgcagaaa aagcccttta cttagcttaa aaaaggctaa actatttcct gactgtacta
     6421 acggttgaat tgttaaaaaa tgctacatat ccttctgttt acttaggata attttataaa
     6481 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
     6541 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
     6601 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
     6661 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
     6721 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
     6781 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
     6841 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
     6901 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
     6961 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
     7021 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
     7081 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
     7141 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
     7201 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
     7261 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
     7321 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
     7381 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
     7441 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccacacga
     7501 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
     7561 gccggaaatc atcgccacca tccacggtga aggttatcgc ttctgtggtg atctggaaga
     7621 ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     7681 accttatttc cagatacgta attgatcgtc cattgtaact tctttactgg ctttcatttt
     7741 cggcagtgaa aacgcatacc agtcgatatt acgagtcaca aacatcatgc cggccagcgc
     7801 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     7861 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     7921 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     7981 tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa acattaccaa
     8041 tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     8101 aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc
     8161 cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     8221 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
     8281 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
     8341 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     8401 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     8461 cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     8521 ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca ccacaccaat
     8581 accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga tatttggcgc
     8641 attgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     8701 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat
     8761 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
     8821 ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa cgagtttttg
     8881 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     8941 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
     9001 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     9061 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     9121 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     9181 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     9241 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     9301 atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatacgacc
     9361 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa
     9421 cgccggttca gcagcaacat taacctcggt gggcgtaaca tgtagagtga tgtttttttc
     9481 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
     9541 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
     9601 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ctacttcagg
     9661 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     9721 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     9781 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg
     9841 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
     9901 gatccaccaa accatgcctg cgccaatcac cagtgcgatc cccaacaaaa tggcgctggc
     9961 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
    10021 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    10081 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
    10141 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    10201 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    10261 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    10321 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    10381 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    10441 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    10501 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    10561 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    10621 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
    10681 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    10741 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    10801 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    10861 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    10921 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    10981 aacgccggat ggagcgccag taactggcgg cacaattcaa agccactgat atcaggcaaa
    11041 ccaacgtcaa gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaaa
    11101 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
    11161 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    11221 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    11281 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    11341 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    11401 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc
    11461 gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt tttcgcccgg
    11521 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    11581 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    11641 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    11701 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    11761 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    11821 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
    11881 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    11941 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    12001 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    12061 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    12121 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    12181 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    12241 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    12301 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    12361 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    12421 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    12481 aacaagaggt attctatacc actgcgttag cccaggatca ggcagatggc tatgtactga
    12541 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    12601 gaaccggcgt gcaggagttt atcctgacag tatacggaac gtgcatgcca atgctcaatc
    12661 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
    12721 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
    12781 actcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acaacccagc
    12841 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    12901 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    12961 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    13021 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
    13081 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    13141 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    13201 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
    13261 tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    13321 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    13381 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    13441 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
    13501 cgctaaaatt cagtccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    13561 tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca gtgaggaaac
    13621 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    13681 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    13741 aaacgccagc cagcgcggcg aggcgcgttc ggctactttg ccgttgcctg cggtgattct
    13801 ggaaaaagtg cgtgaaggtg aggcacttgg tcccgtgatg tcgcgttata ccggtattga
    13861 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    13921 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcataatg cggtgtatca
    13981 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    14041 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    14101 ccagccactg gcgcagctca acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    14161 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
    14221 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    14281 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    14341 gtaaccactc ttggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    14401 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    14461 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    14521 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
    14581 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    14641 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    14701 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    14761 aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    14821 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
    14881 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    14941 gctttgcggg atctccttac cgctggtgta gcgtttgaaa agatcgttgt aagccagcgg
    15001 gaatcgctct tccagatgat cccacagctt cccggcgatc gttgcctgaa cgctaagatc
    15061 ccaccattgg ttgttgaaag cataccgcgc cagttgagcc tgctctgttt ttgacttgct
    15121 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    15181 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    15241 atcaatcttc agctcatact cttcgccgat gcgttgtgca gcaaccatcg ggtagaaacc
    15301 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
    15361 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
    15421 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc gcacacggcg
    15481 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
    15541 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    15601 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
    15661 atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa aatcggtcgc
    15721 gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    15781 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    15841 gttacccgct ttcatcgcca ggcggatacg ctctaaatac gccagcggat cttgtttacc
    15901 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    15961 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    16021 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    16081 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    16141 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    16201 gtcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    16261 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    16321 acgactacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    16381 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    16441 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    16501 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    16561 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    16621 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    16681 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    16741 attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat tatggcgggc
    16801 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttatctg
    16861 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtctattga agaagcggtt
    16921 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    16981 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat tattcaggct
    17041 cacgacggtc ataatctgaa cgtacagctg gagcgtgcgg cggatgcgct acgtctgccg
    17101 gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    17161 cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    17221 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgtggtggcg
    17281 attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga actggaccgc
    17341 ggtgaaggta ttccgtggga aggtaactac tcctcctggc tggagcagaa agatcagcgt
    17401 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    17461 tgggtacgtc agggaactaa aggccgtcag tcgaaaggta aagcacgtct ggcacgcttt
    17521 gaagagctga acagcaccga ataccagaaa cgtaacgaaa ccaacgaact gtttattcca
    17581 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggc
    17641 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat cgtcgggatc
    17701 atcggtccga acggcgcggg taaatctacc ctgttccgta tgatctccgg tcaggaacag
    17761 ccggacagcg gcaccatcac tttgggtgaa acggtgaaac tggcatcggt tgatcagttc
    17821 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    17881 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    17941 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    18001 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    18061 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    18121 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat cctggactac
    18181 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ccgaatacga agagtacaaa
    18241 aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa gcgtattgcg
    18301 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    18361 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    18421 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    18481 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    18541 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    18601 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    18661 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    18721 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    18781 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    18841 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    18901 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    18961 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    19021 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    19081 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    19141 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    19201 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    19261 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    19321 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    19381 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    19441 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    19501 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    19561 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    19621 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    19681 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    19741 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    19801 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    19861 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    19921 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    19981 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    20041 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    20101 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    20161 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    20221 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    20281 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    20341 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    20401 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    20461 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    20521 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    20581 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    20641 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    20701 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    20761 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    20821 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    20881 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    20941 ttaccgccaa tcagaatggc gcttcctggc accacgccgc cgcctagtac gcggtcgaac
    21001 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    21061 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    21121 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    21181 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    21241 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    21301 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    21361 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    21421 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    21481 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    21541 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    21601 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    21661 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    21721 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    21781 acacggctgt gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    21841 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    21901 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    21961 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    22021 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    22081 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    22141 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    22201 gaaagaggca gaccaggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    22261 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    22321 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    22381 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    22441 tggcgctgat gcagggagcg tcatggttta gtcaaaacca ccagcgacaa cgtaatccac
    22501 agctggaaga actggcccgc accctggcgc gtcaggtaac gctgaacgtt gcaccgctga
    22561 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    22621 aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg
    22681 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagcg ggcggctatt
    22741 ttaaccagca gattgtcgaa ccaattgccg ggaaaaacgg accgctcggc tatctgcgcc
    22801 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    22861 ttttacgcct gatgttgctg ctttcactgg caatcggcgt agtgctgacc cgcacgctgc
    22921 tacagggtaa acgtacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    22981 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    23041 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    23101 tggaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    23161 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    23221 ggatggaaga agataacgtc cgcctggcac ggcgcagtag cggtggcggc gcggtgttcc
    23281 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    23341 tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    23401 ggcgtaacga tctggtggtg aaaactgccg aaggtgaccg caaagtctca ggatcggcct
    23461 atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat gccgacctta
    23521 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    23581 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    23641 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    23701 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    23761 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    23821 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    23881 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    23941 gcggcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    24001 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    24061 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    24121 attatcaata taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    24181 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    24241 atcgaaaaga cggaacggtt tagctcaaac atcaaaagca aaatggcaga aatttatggt
    24301 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    24361 tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata atagtaaatc
    24421 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct cccactccgg
    24481 cttcacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    24541 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    24601 gctcgtgagt gcggatgtgg tcagacacgg tacagatggt cagggctttc gcgccaaatt
    24661 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    24721 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    24781 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    24841 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    24901 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    24961 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    25021 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttataag
    25081 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    25141 tttcagcaat atactttgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    25201 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatct acatgttttc
    25261 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    25321 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    25381 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    25441 accggaatgt gttcacgcgt gtgatcagta ccggtccagg tcggatcaca accgtggtca
    25501 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    25561 tcgaacagtt ccagacccgc cgcataacct gcgacgtcgc gacggtggcc ccaggaagag
    25621 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    25681 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    25741 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    25801 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    25861 cgctggaagt taccggcttt gtcgccaata aacggacgag cgataacacg accgatgttg
    25921 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaag
    25981 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    26041 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    26101 tggcagttac cgagataacc aggcaggtta gcgcgttcaa ccagtttatc cagcagctct
    26161 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    26221 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    26281 tacgcgccga taacttcagc gttgccgtcc attcctgccg gaatgaaacc ggtagaacct
    26341 tcatgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    26401 ccgttatcag cttcgccttt ggcacaggct tctgcgatat gacccagggt gtcagccccg
    26461 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    26521 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    26581 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    26641 gcaagtttaa ttgccgcttt caccgctttc gccgcgtcct gccagctgtt ttcgtctttc
    26701 gcgtggataa cagccagcgg acgctgaccg tctacctggt cgcccaaacg cgccatatca
    26761 gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc acccattgca
    26821 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    26881 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    26941 gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    27001 agcactgctt gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagata
    27061 agcatctcca cgcacagtgc catcgtgaca tcaaacagac gcgggttacg atattcaccc
    27121 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    27181 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    27241 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    27301 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    27361 gtgatcagcg ggatggagtc cacggttgcg gtaatatcgc gggtcgcgta gaaacgttta
    27421 tctgccggag ccaatgagct ggtctggccg ataatcgcca cgccgacgtc tttaataatt
    27481 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    27541 agcgtaccgc cagtatgacc gaggccgcga ccagagatca tcggaatata gccgccgcag
    27601 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtcgagtgt
    27661 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    27721 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    27781 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag
    27841 aaacgaattt cttcatcgct cagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    27901 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    27961 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    28021 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcgccgtg gcccagagcc
    28081 ttcagcaggc ttgccagcag gctggaagca ccaaagcggt agtggcgcgc atcggcccag
    28141 tcagcgccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    28201 acgccgcccg ccggtttgaa accaacggtt ttttctacgc ccatatcacg aatcacttcc
    28261 atcatgatgc gcgcgctttc cggcgtcgcg ttcactgcca ctttaccggt agaggttttg
    28321 atgaagtcag cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    28381 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    28441 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcacggtac
    28501 gggaacacca cgtcaacttc atcggcaccg taggcgattg ccgcacgggt ttctgccagc
    28561 gcgatttcga tgtcgtcgtt accgtgtggg aagttagtta ccgtagcaat acggatttcc
    28621 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    28681 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    28741 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    28801 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    28861 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    28921 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    28981 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    29041 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    29101 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    29161 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    29221 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    29281 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    29341 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    29401 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    29461 tgttagaatt ctaacacaag acgagatcga tattcgaata aaacgtttct ggcgttacct
    29521 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    29581 tacccgcgcg atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    29641 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    29701 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    29761 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    29821 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    29881 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    29941 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    30001 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    30061 gtgtgagaaa gaagggattg ccgcacgtta cggtaaagaa gccgccgcaa atgaagtggg
    30121 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    30181 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    30241 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    30301 cccgcatcat gcttattatt attccggcat cagcgacatt ctgaccctcg acgaaaccat
    30361 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    30421 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    30481 tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    30541 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    30601 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    30661 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    30721 ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt
    30781 tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct
    30841 gcctgaagag gtgtccgcaa catgccacgc cgatggcgca atccatgagc gtggacgaag
    30901 tgcttagcca tgtccgcaaa gcggtgctgt ttatcgaagg gataacggtg agtggcggcg
    30961 aagccacgac ccagctgccg tttgtggtgg cgctgtttac tgctatcaaa aatgatccgc
    31021 aactgcgcca tctcacctgt ctggtggaca gtaacggtat gttgagcgag accggctggg
    31081 aaaaattgct cccggtgtgc gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat
    31141 gtcatcaaca aataaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgctgg
    31201 cagagcgcgg taagctggcg gaactgcgtt tgctggtgat ccctggccag gtggattatt
    31261 tgcaacacat cgaagaactg gcggcgttta tcaagggact cggcgatgtt ccggtacgcc
    31321 tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    31381 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    31441 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    31501 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt
    31561 gaacgcctta tccggcctac ggttcggcac agacttgtag gcatgataag acgcgtcagc
    31621 gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta tccggcctac
    31681 ggttcggcac aaacctgtag gcatgataag acgcgtcaag cgtcgcatca ggcattgtgc
    31741 gccaactgcc ggatgcggcg tgaacgcctt atccggccta cggttcggca caaacctgta
    31801 ggcatgataa gacgcgacaa gcgtcgcatc aggcattatg cgccgactgc cggatgctgc
    31861 ctacgaaggg taaactaccc acgaacgcta aacaccgcat acgtattatt aagcaacact
    31921 tcggcaatct catcttctgg ttccggacgt aactcgcaaa gcacgtcaaa cacccgcaca
    31981 gcctgttccg ggcggttagg cttcccctga aagccgttga gcggcatatc cggcgcgtcg
    32041 gtttccagca acaacgatgc cagcggtaat tttgcgatga catcgcgggt tttactggcg
    32101 cgtggatagg tgatagtacc gcctacgccg attttgtagc ccagctgtac aaaccgctcg
    32161 gcctgttgca ggctgccgga aaaaccgtga accacgccag tgcgcgataa atcgtggcgt
    32221 ttaagatgca tcgccagttt gtcgtgtgtg cgccgtgaat gcaggatcac cggcagatcg
    32281 tagcgtttcg ccagtttcag ctgttcgtcg agtaaccact gctgcctctc aagttgcgga
    32341 tcgtcgccga agagatccag accgacctcc cccactgcca ccaccttcgc cggacgcctt
    32401 tccagcgcct gctgtagctg ctcaagagac acatcgctat gtttttccaa catacctgga
    32461 tgcaagccca atgcggcata cagcggttga tatttttccg ctaacgcctg cacacgggca
    32521 aaattttccg cctctgtcgc cggaacaatg atcttgccta cgcccgcttg tgccgcgcgt
    32581 tgcaggctgg cctcttcatc gccactaaac ggcgggaaat caaaatggca gtgggtgtcg
    32641 ataaaacggc aaatcaagcc agatcctcat tgttaaatgt cgggtcgttc gcctgcggag
    32701 cgtcaatcac ttcggcaacc agcgtatcgt tggcgactgg cgcaggcgga ataacgatcg
    32761 attccggcgt caccactggc accagatggc gggtaagcgg cgctttttca gttaatagct
    32821 tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc aagtttatag tcttcacgca
    32881 gcgccggaat ccgactgcca agcgcgatac tatgtaatgg cttcggcgga taaatttcga
    32941 atatccgcag cttgcccggt ggtttctcaa taaattgctg aatgtcacga tagctggttt
    33001 catgatgctg caccagattg accagcggct gcaggctact gtcacccagc cagcgttcca
    33061 tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg cacagtgcga atgacgacca
    33121 acgttttagc gccctgcctt gccgcctctt taaccggaat cgcatcactg atcccgccat
    33181 ccaggtagtt aatgccttcc agcgacactc cgctacgata aaagccaggt atcgcactgg
    33241 aggcgcgaat cacatccagc cagttttgtt tggttggtaa aaagtaatta ggcgcgtagt
    33301 catcctgacg acaggcgcac atataaaacg atttgccgct gtcaaacaac cgcgcggcgg
    33361 tatccatttg caacggcatc tggcttgcgg tggcctccac cagccagtcg agatcgataa
    33421 gatttcctcc acggacaaag cgcaatggat cgaaaaattc gcgttttgtg gtatagcgca
    33481 tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa cgccgagagg ttctgcgccc
    33541 cggcagatgt gccgagataa agatcgaaag gattaaactg cgcgcgcata aactcatcca
    33601 gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc gcacaccaga gctattcgtc
    33661 caggctggaa cggccttagc gacaacggcg caatattacc aagcgttaca ggtattcgct
    33721 gccccacctc tgccttcctg tttttgttgt gaaagaatga caaagtaacg caatttatta
    33781 ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt ggataaaatt ttcttaatgg
    33841 tctgaaatct agggtcgttt tcggcccatg aacaaactca ccaggaacag aataatcccg
    33901 acgacaaaga caattttagc tgcgcctgca gcggtaccgg ccagaccacc aaacccaagt
    33961 gcggcggcga ttaacgcgat aaccagaaat atgatgcccc aacgaaacat aagcgtctcc
    34021 tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa atcactcaat gcgatatttt
    34081 tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag gacgaattac gacgaattac
    34141 ttagttttca gatcattttt aacgcttttc acaccatcta ccgctttggc gatactttca
    34201 gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga gctgaaccac gccgtcggtg
    34261 gtttcaactt tcacatgacg ggaagggacg atatcgtccg ccagcagttt ggctttgatt
    34321 tcactggtgg tggcggtgtc acccgcgtag cccttcacag agccttcttt agcgtcgcga
    34381 acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc ctttcgccac tttcactgcc
    34441 tcttcggcct gagcctggct ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt
    34501 ttgacagaga tatcggtgct cttgatgttg tcatgatcca ccagcgccgc cttcactttc
    34561 gcggtgatgg cgctgtcatc catgaaatta ccgactttat tcatagagct atcgactttt
    34621 tgccctgcgc tttcattggt agtctgtgcg ttgttttccg cgtaggcaga gccggtcgcg
    34681 acggcagagg tcaacattac agccagcaga gttttcgaaa tcttcagtct tgtcatagtc
    34741 atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg gcaacacgag gttgcattgc
    34801 tgaataggga gagacttcac cctctacaga agtcaatggt cgccatcaca aaagcgatgg
    34861 gtgatgaata acgaccatta cagcctctga atcagttatt aatatcggta gaatgacaat
    34921 cgacggcttt agatactgat atctacgcat tgaacggtat ttaacgccgt cagaaatgtc
    34981 attactttgt taaatataga tcacaatttt gaaaccgctc gggatatcag cgaaaacata
    35041 agcaaaagtg aatgttttaa gaacattccg taagcggcta ataaggaagg gaaattgaca
    35101 gggcgcagcg gctaccgcgc cctggaggca agaattaatg ctcgcgggtc tggtggaact
    35161 gaacgtcggg ataacgttcc tgcgccaggc gcaggttgac catgctggta gcgatgtaag
    35221 cgaggttatc gccgccatca agcgccagtt ggctttcgtt cttacgcttg aactcttcga
    35281 atttcttcgc gtccgcacat tctacccagc gggcagtggc aacgttgact gattcatata
    35341 ctgcttcaac gttgtattcg ctcttcaggc gcgataccac cacatcaaac tgcagcacac
    35401 caacagcacc aacgatcaaa tcgttgttgg agatcggacg gaacacctgc accgcgccct
    35461 cttcggaaag ctgtaccagc cctttgagca gctgtttttg cttcagcgga tctttcaggc
    35521 ggatacgacg gaacagttct ggtgcgaagt tcggaatacc ggtgaacttc atcatctcac
    35581 cctgggtaaa ggtgtcgccg atctgaatag tgccgtggtt gtgcaggccg aggatatcgc
    35641 ccggatacgc ttcttcaacg tgcgaacggt cgcccgccat aaaggtcagc gcgtcggaga
    35701 taaccacatc tttcgcagta cgcacctggc gcagcttcat gcctttttca tatttaccgg
    35761 atactacgcg cataaacgcc acgcggtcgc ggtgtttcgg gtccatgttg gcctgaattt
    35821 taaatacgaa gccggtaaat ttgtcttcgc tcgcttgtac ggtacgggta tcagtctgac
    35881 gcggcatcgg cgcaggtgcc cactccacca ggccatccaa catatgatcg acgccgaagt
    35941 tacccagcgc agtaccgaag aataccggag tgatttcgcc cgcaaggaac agctctttgt
    36001 cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc acgcagctgc tgtgccagat
    36061 cttcaccaac cgcagcatcg agatccgggt tattcagccc tttaacaatg cggacttcct
    36121 gaatggtgtg ccctttaccg ctctgataga gataggtttc atctttataa aggtggtaaa
    36181 cgcctttaaa cagcttgccg cagccaattg gccaggtgat cggtgcgcag ccaattttca
    36241 gctcgttctc aacttcatcg agcaattcca tcgggtcgcg gatatcacgg tcaagtttgt
    36301 tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac
    36361 gatcttcaac accttttgcg gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg
    36421 tacgataggt atcttccgag aagtcttcat gccccggggt gtcgagcagg ttaaccaggc
    36481 aatcgtgata cggaaactgc atcacagacg tagtaatgga gatcccacgc tgcttttcca
    36541 tctccatcca gtccgactta gcgtgctggt tggaaccacg gccttttact gtaccggcgg
    36601 tctgaatggc ctgtccgaac agcagcacct tctcggtgat ggtagtctta ccggcgtccg
    36661 ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc ttgcaaataa ggagacaacg
    36721 tcataattct ttcttcttga gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt
    36781 gcggctattt tacccatcaa cgggggggag gcaatcagtg tttacacagg agctgctcca
    36841 gttcatgcaa cgaagaaacg gtccaggtgg gcgcgatgcc ttctggttgc tcgcgatggt
    36901 gtgcattcag ccagcaggtc gcaagcccgg cgttgatgcc accgagaata tcggactcgg
    36961 cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt gcccgcctgt tccagcgcat
    37021 aatcgaatat tttcttattc ggtttagcaa cgccaacttc ttcggaaatc accagcaaat
    37081 cgaagtaatc acgcaggccc gtccgttcca gacgcacttg ctgcaaggcg ctaaaaccgt
    37141 tggtgatgat gccgatttta gcgttgccac gaatggcgtt aagcagagaa accgcgcccg
    37201 gcaacggcgt acagatttcc gccatcgcat taataaaggc atcgttaagc aagcctgctt
    37261 caactttcag ccgttcggcc cagctctcaa accgcccgtg ctgaagctgt aatgaagtga
    37321 tcgcgccgtt ttgataatcc acccacagtg gcttgttaac ggcctgatag tcctgaaaat
    37381 cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg ctgcaggccg gtgaatgagt
    37441 caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca gtcccacttc attattccac
    37501 cttgtcttac atactgattg gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt
    37561 ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct aaactttcgt acagggcaat
    37621 ggcggcagcg tttgaagcac ggacttccag ccatagtgtc gccacgccgc gtttttccag
    37681 ttcgtcgatc agatgttcca gcagcaccct tcccaatccc tgacgctgat agtcaggatc
    37741 gaccgcaata ttgaacaatg tagcttcatc cagcaccact tgcgtaatcg caaacgccgc
    37801 cattttgccg ttttgcgtta actgaaagtt gagataacgc tcgccctggt tgctggcaaa
    37861 ggttttttca ctccacggaa aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa
    37921 atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca aatctgttgc cataacgcgg
    37981 cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt
    38041 ccagtgatag cggttcgtca gtacccaacc gccagctgtt gcagcgactg ccttgcggca
    38101 gcatcgcgat tttttctggc gtcagttgca gcacttgatc ggggctgacg gttaatgcgc
    38161 gcagaacatc gctcactaaa ggatcggtca gggcgggaag atcgtttgcc accatcacca
    38221 gacggacgtg tgccgggatc gcaatggcaa tctccccctg caacgcgcca gggcgacgca
    38281 gcgaccactg ggtaatgccc agttgctgta actgccagtc tcgtcgggat gtcatagcga
    38341 atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt cgataccgcg ccaacatatg
    38401 gctataatcg ccgccagtat caattgagga gcattccatg tctgcattta ccccggcaag
    38461 tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc cgtattctgt ttgccggaga
    38521 cttacaggat gacctgcccg cgcgtttaga taccgcggcc agccgtgctc atacccagca
    38581 attccaccac tggcaggtat taagccgcca gatgggggat aacgcccgtt tcagtctggt
    38641 cgccacggtg gatgacgtcg cagattgcga tacgctgatt tactactggc cgaagaacaa
    38701 accggaagcc cagttccagt tgatgaattt actttctctg ctgccagtgg ggacagatat
    38761 ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgcc gagcagatgc tggcagatta
    38821 tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc ctctattttg gtcgtctgga
    38881 aaaacagccg gtatttgatg ccgataaatt ctggggcgaa tacagcgtcg atggcctgac
    38941 ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta gccagttgct
    39001 gctctcgacg ttaaccccgc acacgaaagg taaagtgctg gatgtcggct gtggcgcggg
    39061 tgtgctttca gttgcctttg cgcgccattc gccgaaaatt cgtctcacct tgtgcgatgt
    39121 ctctgcgcca gcggtagaag ccagccgcgc aacacttgcg gccaacggtg ttgaaggtga
    39181 agtctttgcc agcaacgtct tttccgaggt gaaaggtcgt tttgatatga tcatctccaa
    39241 cccgccgttc cacgatggga tgcaaaccag cctggatgcg gcgcaaacgc tgattcgcgg
    39301 cgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc
    39361 ttacccggac gtgctggatg agacatttgg cttccatgaa gtgattgcgc aaaccgggcg
    39421 tttcaaggtg tatcgcgcca ttatgacccg ccaggcgaag aaaggttaat tatctcgccg
    39481 aataccgtgt cggatgcggc gtgaacgcct tatccgacct acaaaacctg cacgttagcc
    39541 cttcgtaggc cagataagac gcgccagcgt cgcatctggc atctgccccc gcatgcctac
    39601 caaacctgca cgctagtttc ctgatggaca tttttccagc aattacacct ccggcgataa
    39661 ttaactattg acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt
    39721 taagaattga tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag
    39781 tgtccttacg gacgtggggg ttcaagtccc ccccctcgca ccaattatct ttacttcctt
    39841 tcttgtttct tccttgattt ttcatccctg attcaaattc attatcatca gcatgatgcc
    39901 agcaataagc gccaggcacg atggtagcaa aacgtaatgc cgtaactgtt tgtgatacag
    39961 catcaccggc gtcaatacta atcccatcag aatgacaatc cgccactgat ccggcgataa
    40021 atccgcatac atcagccctg caatcagcaa tcctggcagc aacactcccc agccactgcc
    40081 cagcatacgt tgcaacatgg tttcatctct ttcattgata atgataacca atatcatatg
    40141 ataattttta tcatttgcaa gccagagaaa tcccttgcta tcgggtaaac ctatcgctag
    40201 gattagcaat cattatcatt tatattacta tcccgattat ggcctatcgt tccgcaccgc
    40261 tctatgaaga tatcatctgg cgaacgcatc tccagccgca ggatgctggg cttgcacaag
    40321 ccgtacgtgc gacgatcgcc aaacatcgtg aacatttgct cgagtttatc cgcctggatg
    40381 aacccgctcc gcttaacgcc atgacgctgg cgcaatggtc atcaccgaat gcgctaagtt
    40441 ctctactcgc ggtctattcc gatcatatct atcgcaacca accaacgatg atccgcgaga
    40501 acaaaccgct gatctcacta tgggcacaat ggtatatcgg cctgatggtg ccaccattaa
    40561 tgctggcgct actgacgcag gaaaaggcat tagatgtgtc gccggaacat ttccatgctg
    40621 agtttcacga aaccggccgt gccgcctgtt tctgggtcga tgtgtgtgaa gataaaaacg
    40681 caacaccaca ttcgccgcag cagcgaatgg aaaggttaat cagccaggcg ctggttccgg
    40741 ttgtgcaggc actagaagcg accggagaga tcaacggcaa acttatctgg agtaataccg
    40801 gttatttgat caactggtat cttactgaga tgaagcaact gctcggcgag gcgacggttg
    40861 aatcgctgcg ccatgccctc ttttttgaga aaacgctcac gaacggtgaa gataatccac
    40921 tctggcgtac cgtggtactg cgcgatggcc tgctggtgcg ccgcacttgc tgccagcgtt
    40981 atcgcttacc ggatgtgcag caatgtggcg attgtacgct gaaatgaaag cactgccggg
    41041 gaagtaaacc cggcatcatg cggattaata gggatgcaac acattacttg tttcgcaatg
    41101 ccgcatacat aacagaatat ctgcggcctc caacaaccct gcatcagaac tgacgcccag
    41161 tttcgacatc acattaaatt tatgtgcacg gatcgttttg atattacgtt taagctgctc
    41221 ggcaatttgt gtcattgagt agccacgcga cataaagcgc aatatttctc tctccgtcgg
    41281 gcttagcgtc cggctttggt taatgtacca ctgattgttc agtcggtcgg tcgcctgacg
    41341 tacaccattt aatgacaaaa agagttcctg atgaaaaatc tccagcgttg acgctttact
    41401 taatacaccg tccagcggtg atggcgacaa tgaaccaatc agccgagctt caatatcatc
    41461 atccgcaata actaaacgcc gggtacgcgg aaacttaatc gccagttcag tcaggcaaga
    41521 taatccctct ctgcgctcac ttctcatggc agaaaaagaa aaaatgaccg ccgtaaacga
    41581 gatccgcgac atggcatcct gaaatgactc ctgactggta taggtatgga gcttataagg
    41641 gctacccgca aactttctga ataaactctc aataccgata ctactcatga cgcatttttc
    41701 tatgagtgcg acatttctct tcttaattcg gctgtgttcc attctgcggc atctccataa
    41761 acagaaggca agttaagttc tcttgcaccc tgcgcgctat ttatccatgc atacatttca
    41821 gcgttgctgt gaattgccag tcgacgcatc gcactgtttt tttgggcgct gatagtttta
    41881 ttacttttct taagcaatga agctatctgg ttgatgcccc atcctttacc taatagccgc
    41941 agtaccttgc gctctgaaag agtgagaatg acggagcgat agctaaaatc atgagtttca
    42001 gtcattgccg gagaggtcag catggcgctg atacgctctg cgtgcgtatt gccggaacgt
    42061 acggtcttga ccagattctc aatgggttca acatcagaca ataacgtcgc cgtagggcac
    42121 atgagcagtt ctactgcctg ggaataccag gagcgtgaca ccatgaaaac ccagtgaatt
    42181 tcccgatatt gtgagattaa agaataataa tgttcacaaa cagaacgtgg atcttcactt
    42241 tcaccggcta aatcagcaat gactaatccg gaacgacgta attgtaataa ggtaaggtcc
    42301 tccgcagagg cgctggatat tatttcatat tcagggaagt gagtcctcat gacctctttt
    42361 agccctgctt gcataacagg tattttactg ataacaattc catttttgca gcatcctggc
    42421 aacatatttc ctccacatcc actgtctaaa aattatgatg gtttattcag gatattcatt
    42481 ccttaaatgt ttccagcaaa ttaatagatt aagcgaataa attatcctta gcattcactt
    42541 tcatatcagc atactcctgc tgcacaacaa ctccactgca tgtgtcagaa ggttagcatc
    42601 aaatgataa
//
LOCUS       DAZGCS010000033        42607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGCS010000033 DAZGCS010000000
VERSION     DAZGCS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.033"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..1377
                     /gene="ybdN"
                     /locus_tag="ACHK01_001843"
     CDS             157..1377
                     /gene="ybdN"
                     /locus_tag="ACHK01_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8096805.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1350..1979
                     /gene="ybdM"
                     /locus_tag="ACHK01_001844"
     CDS             1350..1979
                     /gene="ybdM"
                     /locus_tag="ACHK01_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8096806.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1980..3140)
                     /gene="ybdL"
                     /locus_tag="ACHK01_001845"
     CDS             complement(1980..3140)
                     /gene="ybdL"
                     /locus_tag="ACHK01_001845"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO8096807.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PHYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3249..4337
                     /gene="hcxA"
                     /locus_tag="ACHK01_001846"
     CDS             3249..4337
                     /gene="hcxA"
                     /locus_tag="ACHK01_001846"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO8096808.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNYLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4347..4544)
                     /gene="ybdD"
                     /locus_tag="ACHK01_001847"
     CDS             complement(4347..4544)
                     /gene="ybdD"
                     /locus_tag="ACHK01_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8096809.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4726..6831)
                     /gene="cstA"
                     /locus_tag="ACHK01_001848"
     CDS             complement(4726..6831)
                     /gene="cstA"
                     /locus_tag="ACHK01_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO8096810.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLIWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7012..7425)
                     /gene="entH"
                     /locus_tag="ACHK01_001849"
     CDS             complement(7012..7425)
                     /gene="entH"
                     /locus_tag="ACHK01_001849"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO8096811.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7428..8174)
                     /gene="entA"
                     /locus_tag="ACHK01_001850"
     CDS             complement(7428..8174)
                     /gene="entA"
                     /locus_tag="ACHK01_001850"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO8096812.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTAFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8174..9031)
                     /gene="entB"
                     /locus_tag="ACHK01_001851"
     CDS             complement(8174..9031)
                     /gene="entB"
                     /locus_tag="ACHK01_001851"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO8096813.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9045..10655)
                     /gene="entE"
                     /locus_tag="ACHK01_001852"
     CDS             complement(9045..10655)
                     /gene="entE"
                     /locus_tag="ACHK01_001852"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO8096814.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTLVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10665..11840)
                     /gene="entC"
                     /locus_tag="ACHK01_001853"
     CDS             complement(10665..11840)
                     /gene="entC"
                     /locus_tag="ACHK01_001853"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO8096815.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12215..13171
                     /gene="fepB"
                     /locus_tag="ACHK01_001854"
     CDS             12215..13171
                     /gene="fepB"
                     /locus_tag="ACHK01_001854"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8096816.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHK01_001855"
     CDS             complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHK01_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO8096817.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14536..15540
                     /gene="fepD"
                     /locus_tag="ACHK01_001856"
     CDS             14536..15540
                     /gene="fepD"
                     /locus_tag="ACHK01_001856"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO8096818.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15537..16529
                     /gene="fepG"
                     /locus_tag="ACHK01_001857"
     CDS             15537..16529
                     /gene="fepG"
                     /locus_tag="ACHK01_001857"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO8096819.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLSWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16526..17341
                     /gene="fepC"
                     /locus_tag="ACHK01_001858"
     CDS             16526..17341
                     /gene="fepC"
                     /locus_tag="ACHK01_001858"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8096820.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK01_001859"
     CDS             complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK01_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO8096821.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(18687..22568)
                     /gene="entF"
                     /locus_tag="ACHK01_001860"
     CDS             complement(18687..22568)
                     /gene="entF"
                     /locus_tag="ACHK01_001860"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO8096822.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADAHYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22565..22783)
                     /gene="ybdZ"
                     /locus_tag="ACHK01_001861"
     CDS             complement(22565..22783)
                     /gene="ybdZ"
                     /locus_tag="ACHK01_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO8096823.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22786..23988)
                     /gene="fes"
                     /locus_tag="ACHK01_001862"
     CDS             complement(22786..23988)
                     /gene="fes"
                     /locus_tag="ACHK01_001862"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO8096824.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24231..26471
                     /gene="fepA"
                     /locus_tag="ACHK01_001863"
     CDS             24231..26471
                     /gene="fepA"
                     /locus_tag="ACHK01_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO8096825.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYVTTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSKNKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26511..26609
                     /locus_tag="ACHK01_001864"
                     /pseudo
     CDS             26511..26609
                     /locus_tag="ACHK01_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26646..27266
                     /gene="entD"
                     /locus_tag="ACHK01_001865"
     CDS             26646..27266
                     /gene="entD"
                     /locus_tag="ACHK01_001865"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO8096826.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27388..27540)
                     /gene="hokE"
                     /locus_tag="ACHK01_001866"
     CDS             complement(27388..27540)
                     /gene="hokE"
                     /locus_tag="ACHK01_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO8096827.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27891..28043)
                     /gene="hokE"
                     /locus_tag="ACHK01_001867"
     CDS             complement(27891..28043)
                     /gene="hokE"
                     /locus_tag="ACHK01_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO8096828.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            28012..28281
                     /locus_tag="ACHK01_001868"
     CDS             28012..28281
                     /locus_tag="ACHK01_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8096829.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28485..29603
                     /gene="ybdK"
                     /locus_tag="ACHK01_001869"
     CDS             28485..29603
                     /gene="ybdK"
                     /locus_tag="ACHK01_001869"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO8096830.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29669..29917
                     /gene="ybdJ"
                     /locus_tag="ACHK01_001870"
     CDS             29669..29917
                     /gene="ybdJ"
                     /locus_tag="ACHK01_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO8096831.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29982..30350
                     /gene="ybdF"
                     /locus_tag="ACHK01_001871"
     CDS             29982..30350
                     /gene="ybdF"
                     /locus_tag="ACHK01_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8096832.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALIRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30444..31097
                     /gene="nfsB"
                     /locus_tag="ACHK01_001872"
     CDS             30444..31097
                     /gene="nfsB"
                     /locus_tag="ACHK01_001872"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO8096833.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31205..32452
                     /gene="ybdG"
                     /locus_tag="ACHK01_001873"
     CDS             31205..32452
                     /gene="ybdG"
                     /locus_tag="ACHK01_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO8096834.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32520..33896)
                     /gene="pheP"
                     /locus_tag="ACHK01_001874"
     CDS             complement(32520..33896)
                     /gene="pheP"
                     /locus_tag="ACHK01_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO8096835.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33998..37141)
                     /gene="cusA"
                     /locus_tag="ACHK01_001875"
     CDS             complement(33998..37141)
                     /gene="cusA"
                     /locus_tag="ACHK01_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO8096836.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37153..38376)
                     /gene="cusB"
                     /locus_tag="ACHK01_001876"
     CDS             complement(37153..38376)
                     /gene="cusB"
                     /locus_tag="ACHK01_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO8096837.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38392..38724)
                     /gene="cusF"
                     /locus_tag="ACHK01_001877"
     CDS             complement(38392..38724)
                     /gene="cusF"
                     /locus_tag="ACHK01_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO8096838.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38748..40130)
                     /gene="cusC"
                     /locus_tag="ACHK01_001878"
     CDS             complement(38748..40130)
                     /gene="cusC"
                     /locus_tag="ACHK01_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO8096839.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40287..40970
                     /gene="cusR"
                     /locus_tag="ACHK01_001879"
     CDS             40287..40970
                     /gene="cusR"
                     /locus_tag="ACHK01_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO8096840.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40960..42408
                     /gene="cusS"
                     /locus_tag="ACHK01_001880"
     CDS             40960..42408
                     /gene="cusS"
                     /locus_tag="ACHK01_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO8096841.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10170 a        11778 c        10900 g         9759 t
ORIGIN      
        1 gagtttttga tatataacta cagacagcta ctataacttt catctattta ttcacagcgc
       61 gaatgaaaaa taaaacactc tgctttgcag tgtttacttt ataattaaca gacttatctc
      121 actgatcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
      181 ccgctcaata ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc
      241 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
      301 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa
      361 ttctcttgca ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa
      421 gagttttact gggttgcgct cccgcttacg acgcaaaatt ccctttcaca attccaaccc
      481 gaatggcagt gctgggaacc tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata
      541 accgatcctg actttttctg cttttaccag cctggcatga ccttcgaaca atttgtacgt
      601 gagtttgccg aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg
      661 gatgagtcct acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac
      721 gataaaccat ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac
      781 gactggaaag tggctgatat ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca
      841 ctgtataacc taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct
      901 tttggcccgg agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg
      961 gcagctatgt gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgac
     1021 aatcatttct atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat
     1081 gcattattgc tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc
     1141 gccatttatt tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg
     1201 gacattggcg cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac
     1261 gattactggt gtcgagcatt atcatttagt cctacgaaag cgaagaacta tcagcgttat
     1321 aacgaacgga taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc
     1381 aggatttaac gcaatttctc gccagcttgc cggaagatga tcgcatcaaa gcgattaatg
     1441 aaatccgcat ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc
     1501 tgtgggttaa aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac
     1561 ccgaaaaaaa actgctgaaa aaatccattg aaatagatgg ttttacccaa cccattgtgg
     1621 tcacgcatac ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc catgaaattg
     1681 gtaaaggctc atcatcatta aaattacgct taaaaggtta tttgccggtg acctgtctgg
     1741 aaggtacgcg taatcaacgc atcgccgcga ctattcgtca taatcgcgct cgtggacgcc
     1801 atcaaatcac cgccatgtcg gaaatcgtcc gtgaacttag ccagttggga tgggacgata
     1861 ataaaattgg caaagagctg ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta
     1921 acggcctgca agagttgttt gccgaccgtc aatattcccg cgcctggacg gttaaatagc
     1981 taaagctggc gcaggcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag
     2041 agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct
     2101 acgccgtgct cctgcgtcag ccactggcaa aactcaacat catccagggt agaaaccgcg
     2161 ctgtaatcca ccagcaaaaa gtatgtacct tcacacggta aaatctccag ccggctttca
     2221 tttaaagcat tcaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa
     2281 tgctcaggtt ctgcacgtag catatcagca agcgccagct gtgccggggt attcaccgaa
     2341 aaggtcagat actgatgtac cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa
     2401 taacccactt tccagccggt catatgatag gtcttgccaa atgaagaaac cgccactgcc
     2461 cgctcacgca gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg
     2521 tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa
     2581 gcggcgaaat cagcctgctg ccagatagtt gcactggggt tatgcggagt gttgaggatc
     2641 accagtctgg tgcgctcgct taataatgcg gcaaattcct gccagtcaac gcgaaaatgc
     2701 ggtggttgca gtgccatacg cttcactatt cccccagaaa gcgcgatggc gggggagtaa
     2761 ctgtcatagc tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc
     2821 gccgcgtata acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt
     2881 tgatagccat acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg
     2941 gtcatgggcg cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa
     3001 tagtgcggac catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc
     3061 gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga
     3121 atcagagggt tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat
     3181 aacacgatgc tagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac
     3241 aacacaacat gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac
     3301 atccaggaag tttcaattac ctgaacgatt ttttcactga tgaacaactt tctcgcgcgg
     3361 tgtggatcta cggcaaacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg
     3421 gacttccagg ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc
     3481 aactggcggc tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac
     3541 tgctcgacac cgcgaaagcc ctcgcccgcc gtctcggtct accatttgtt gccgctccga
     3601 cgatcgccgc cacctgcgca gcctggacac cgctctccgt ctggtataac gatgccggac
     3661 aggcgctgca ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccggaga
     3721 ttatcctcaa tgcaccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat
     3781 ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcggctgg
     3841 ggatcaataa tgcgcaggcc attcgcgacg tcttgttaaa cagtagcgaa caggcacttg
     3901 ccgatcagca aaatcagcag ttaacgcaat cattttgcga tgtggtggat gcgattatcg
     3961 ccgggggtgg aatggtcggt ggtctgggcg atcgttttac gcgtgtggct gcggcccatg
     4021 ccgtgcataa cggtctgact gtcctgccgc aaaccgagaa atttctccac ggcaccaaag
     4081 tcgcttacgg aattctggtg caaagcgcct tgctgggtca ggatgatgtg ctggcgcaat
     4141 taaccggggc gtatcagcgt tttcatctgc cgactacgct ggcggagctg gaagtggata
     4201 tcaataatca ggcggagatc gacaaagtga ttgcccacac cttgcgcccg gtggagtcca
     4261 ttcattacct gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg
     4321 aatcgtttaa agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag
     4381 cgcgcgtcct gccgctcacg gaaaaactcc tcgtaggtca tcggcgtttg atcgggatgg
     4441 ttaacccgca tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc
     4501 gcctgaccta aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac
     4561 catgttgctg aaagagtgcc ggatgcggcg tgaacgcttt atccggccta caaatgctat
     4621 caacgcactc ggtcagtccc ctcgccccat cggggagagg gttagggtga gggggaaccc
     4681 tcggcactat gccaacattc gccaacatcc cccctcactc tgactttagt gcgcaccttt
     4741 cgcctgcgct acgatctcct caacattttc cggcattggc tcatacggcg tttctttcgc
     4801 cgttggcttc ggctctttca atgccgcaag tgccgtctta atcgagaaca gtgccagaac
     4861 caccacgacc accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag
     4921 ttgcgccagc tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg
     4981 gaacttgtta gcaatggcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca
     5041 gcctgcggtc agggtacaaa tcagtagcca ggccgttggt accagcgcca cccaggcgta
     5101 acgttgacgt ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag
     5161 catctggttg gcaataccaa acagcggcca cagagtgtta ataccgccca gcggatcgac
     5221 cacgccctga tggaggaagt agccccacgc cagtacgcac agcgccgttg ccagcaggtt
     5281 agcaggcagt gaatcggtcc gcttcaggcc aggagacacc acgcccagca gatcctgcaa
     5341 cataaagcgc gcagcacgcg tacccgcatc taccgccgtc agaataaaca gagcttcaaa
     5401 cagaatggcg aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag
     5461 gatgtaagcc atccccaccg ccaacgtcgg cgcaccgccc gcacgggaaa tgatcgactg
     5521 ttcacccact tcgctggcaa tctggttgag cgtatctggt gtaatgctaa agccccagct
     5581 actcaccacc tgcgcggcag aagcgaccac atccgccgtc cctgccggag ccagcaccgc
     5641 cattgggctg ttcatggcaa agtaaacgcc cggatcgatg atacaggcgg aaaccagcgc
     5701 cataatcgcc acgaaggatt ccatcaacat tccaccgtag ccgataaaac aagcctgccc
     5761 ttcgttcgcc agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac
     5821 cgcgccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc
     5881 agtgccatcg acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat
     5941 gcctaccgcc agaccgacga tcgtcccgat tttcaggaag gtagagaggt agtcacgcgg
     6001 ggccagcagc aaccacaccg gcagcaccgc cgccacaaaa ccgtaaccca ccagcatcca
     6061 gatcagctgc acgccggtaa agtcaaagta cggtgcccag gtcggacttt ctgccaccca
     6121 gccgccagag ataatggcga aaatcaggaa taccagaccg atgaccgaca cttcaccaat
     6181 acgccccgga cgcagatagc gcaggtagat ccccataaac agcgccagcg gaatggtgaa
     6241 cgcaacggta tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag
     6301 tactgcaagg ataatgacca tgatcataaa gcaggccacc agcgctatca ccccggcggt
     6361 tggccccatc tcttctttga ccagctcacc cagcgatcga ccgtcacggc gcgtagaaac
     6421 aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga gcagccagat
     6481 catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc
     6541 ggcaatggcc gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag
     6601 cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt
     6661 ggcgatatac agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac
     6721 aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc
     6781 tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat
     6841 ccgtgtgcgt gtccatagat atgttacatt ttgtttctat aacatctttg tacgatttaa
     6901 caacacggta accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc
     6961 gatcactccg ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa
     7021 aactgccgta cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat
     7081 ttcccagctc tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc
     7141 ttcagacacc gggcgatgat gggtggcgtt aagttccgta cccaccacac actgcccgtc
     7201 acgggtcatc ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ctgacgcgcc
     7261 gccatgcagc aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag
     7321 cacatcatct cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct
     7381 ggtggcgttc agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc
     7441 gttgagccgc catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg
     7501 aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg
     7561 agtttaaact gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac
     7621 agcgtgcgtt gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg
     7681 ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg
     7741 ccataagcac tcatgccaat acgcggcgtg tgcgcggcgt cggacgccac agtgacaatc
     7801 gccccgcccc gctgacggcg aaactggttc atggtttgct ggaacaggtt aaacgcaccg
     7861 ccgacgttaa ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcgccc
     7921 atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc gctccgtttc agctaacagt
     7981 cgctgacata cctgcgcgac ctgcgcagca tcggcaacat ccatcacttc ggtcgcaaag
     8041 ggatattgct cctgagcgaa cgcttgatca aaagcggtaa ctttcgctcc cgcctcaaca
     8101 aacgccagcg ccgtggcgta gccgatacct ttacctgcgc cggttaccca gacattttta
     8161 ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg
     8221 ggtttttcgc cagcatgaca aagtcgatat caccatgcac tttgcgccag cgcgccgcca
     8281 gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt
     8341 catcggactc gtccagcaac ggcaggataa cctcacgcag cgccgctttg ctggcgggga
     8401 caggtgctgg cagtaattct tcagtcatca ccacccggcc agaccgcccg gccacatatt
     8461 tcagcgacat caaatgctcg tcacggctga aatcggccag cgcatccgcc accataaacg
     8521 gtttaatatc gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata
     8581 ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac
     8641 gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc
     8701 gatccaccac cttttgctgt tccggcgaac gggtcaggcc cggcccccac atatcattca
     8761 acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accggaatat
     8821 tgtgctgttt gcagtagtcg cgcagcgcag caatattcgc gatcacctgc tccatcatcg
     8881 ggcagttctc gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg
     8941 cacgttgcgg ttcaaaggcc cagtcaactt tattcttagg gatatcgtga gactccggca
     9001 gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt
     9061 gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt
     9121 gaatctacac actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg
     9181 aaacgacgca cctgcaccgc gcgcagcggc tcttttacca ccagataagc gcagcttttt
     9241 tcgcccatca gctcatcttc catgctcacc agtgcggcgt agatcaccgc cgggtggcgc
     9301 agcagcaggt tttcgatctc ttcggcagcg atcttctcac cgccacggtt gatctgatct
     9361 ttctcgcgcc cctgcacggt gatgtacccc tctggatcaa tagagatcag atcgccggaa
     9421 cagtaaaaac cgttggcatc aaaggcactg gcattgtgct gcggactttt gtaataaccg
     9481 cggaaggtgt acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg cagtggattt
     9541 ccttcagcat cagcaaccca tacttcatcg tccggacaca ttgggtaacc ctgggtatgg
     9601 ataattttct ccgcgctatc atcaagtcgg gtgtagttca ccagcccttc cgccatgcca
     9661 aacacctgct gcaacaggca gccaatctca gcgggaatac gcgccgcaag ggtggctgaa
     9721 agacgtgcgc cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcacct
     9781 tcggtcagcg cctgcaacca caggctgact gccggtggca ccagcgcggt gacgttcacc
     9841 tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg gatcggccgc cagaacaagc
     9901 gttcctccgg caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc
     9961 gccgggatcg cgcacaggta gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga
    10021 cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg
    10081 ccgccggaaa gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaatcc
    10141 tcagccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc
    10201 accacgcgaa tggaggaatg ttctgtgacg aaagtattga ggaaatcatc cccgctaaac
    10261 agcgcatgtt ggcgatcggc aatcagcaat gcgggttcaa tctggctggc ataggcgttc
    10321 agttcactac gctgatggct gaacagcgcc agcaccggcg caacgcccag tttcagcagc
    10381 gcgaaaaagg taatatacaa ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca
    10441 ggtttaatgc cctgacggcg taaactacac gcgaggttat ccgccgcctg attcagctcc
    10501 cggtaactca actgtcgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga
    10561 gtcagaatgt cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca
    10621 aactcttccg gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa
    10681 acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac
    10741 gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag
    10801 cggatggtca ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca
    10861 aacagttcgc ggtcgaatgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg
    10921 aaaccgctca gcgcgggggt tggatgcagc agacaggcca gagtcagtgc gttttcttgc
    10981 gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat
    11041 tgtggagagg agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc
    11101 tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct
    11161 tcgcgatcga gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta
    11221 aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag gacgccacca
    11281 tcagccagcg ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc
    11341 aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact
    11401 ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg
    11461 gtttgttcag gaattgcctg gcgttccacc acgttcagcg actggctgcg ggtaaaacgg
    11521 cgggctgagg tctgtttttc ctggcgggag aacgactgcc aggattcggg aatatacagc
    11581 gacgaaggct gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc
    11641 tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa
    11701 tccccgttca cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg
    11761 tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc
    11821 tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta
    11881 tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt
    11941 aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt
    12001 attgatggat tcgcataagc gcaatgtgat gtcctgcgcc gttctgcccc ctctcccttc
    12061 cagggtgagg gctggggtga gggttaatgt tcgcaccagt gctggctgtt cccctcaccc
    12121 taaccctctc cccaaagggg cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac
    12181 gcccttaacc ttattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa
    12241 cgcccttcta ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgctgactg
    12301 gccgcgtcag attactgaca gccgtggcac acatacactg gaaagccagc cgcagcgtat
    12361 tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc
    12421 cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg
    12481 gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcgccga
    12541 agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc
    12601 gctggcactg tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga
    12661 caaaagctgg cagtcgctgt taacgcagct tggcgaaatt accgggcatg agaaacaagc
    12721 ggcagagcgg attgcacagt ttgataagca actggcagcg gcgaaagagc aaatcaaatt
    12781 accgccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg
    12841 gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt
    12901 gcccgcaggg ttaaacgcca gccaaagcca gggcaaacgc catgacatca ttcagcttgg
    12961 tggggaaaat ctggctgctg ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca
    13021 gaaagatgcc gatgcgattt atgccaatcc actgctcgcg caccttcccg cagtacaaaa
    13081 caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccatgca
    13141 agtgctggaa cgacttaagg cgctgtttta agcattaact gtcagacgct gtcacctgcg
    13201 gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa
    13261 tcaacaaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac
    13321 cacccagcag cgccgcgcct atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt
    13381 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg
    13441 aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    13501 gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata
    13561 accctggtcg cgcactatgt gccagcttcc cgctggttaa cgcaccaata gccgcgccga
    13621 gcgggatcgc cgcgtagaga aaaccaatct gtgccgctga catctgccag ttgtcagcca
    13681 gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa
    13741 tcccgcccac cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg
    13801 gatgctcacg cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg
    13861 taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca
    13921 ataaaccgcc aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    13981 ccccggcctg cattaagttt tcacgcccta ccagtgcagg tgtcgccgcc agtagcgccg
    14041 taacgccaag tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg
    14101 acggttcagg cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc
    14161 gcgccagcaa aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccgaccatca
    14221 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat
    14281 gtgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga gaaacaatag
    14341 agatgaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    14401 taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    14461 ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt
    14521 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    14581 ttgctgttat tactgattat cgcgacggca ttaagtctgc tcattggggc aaaatcactc
    14641 cccgcttccg tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc
    14701 gtgctcgacg cgcgtctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc
    14761 cttgccgggg cgttaatgca aaccctcacc cgtaacccac ttgccgaccc cggcttgctt
    14821 ggcgtgaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactct
    14881 tccgcacagg aacaactggc gatggccttc gccggggcgc tggtggcctc gttgattgtt
    14941 gcctttaccg gcagccaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc
    15001 gtggcgctgg cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac
    15061 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc
    15121 ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ctgcgctatt actgagtcgc
    15181 gcgctgaaca gtttgagcct cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg
    15241 cgcacacagt tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcagta
    15301 gttggcccga ttgcctttat tggcctgatg atgccgcata tggcgcgttg gctggtgggt
    15361 gccgatcatc gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt
    15421 gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg
    15481 tttattggtg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga
    15541 tttacgtctc tcgccgatta ctcatcacct gtttgctgct ggtttccgcc tgtgtggttg
    15601 caggtatctg gggattacgc agcggtgccg tcacgctgga aacctcgcag gtattcgccg
    15661 cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    15721 gcgtgctgat ggcgctgttg attggcgcag cactgggcgt cagtggcgcg atttttcagt
    15781 cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga
    15841 gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatc gcgctgtcag
    15901 caatggtggg cggcattgtc acttcgctgc tggtctggct gctctcctgg cgcaacggca
    15961 tcgacacctt tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta
    16021 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg
    16081 ccggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat
    16141 tgatgctcat tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg
    16201 ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg
    16261 cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc gatttccttt attgctttag
    16321 tcgcaccgca cattgcccga cgcattagcg gcaccgctcg ctgggggcta acccaggcgg
    16381 cgctatgcgg ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc
    16441 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct
    16501 tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca
    16561 gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga
    16621 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt tactgcgtac
    16681 cttaagccgc ctgatgacgc ctgctcatgg gcatgtctgg ctggatggcg agcacattca
    16741 acattacgcc agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac
    16801 gccgggcgat atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct
    16861 gtttacccgc tggcgcaaag aggatgaaga agcggtaacg aaagcaatgc aggccacggg
    16921 aataactcat ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgcgc
    16981 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac
    17041 ctggctggat atcagtcatc agattgattt gctggagttg ttaagcgaac tgaaccgcga
    17101 aaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag
    17161 ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt
    17221 cactgctgaa ctgattgagc gcatttatgg tctgcgctgc atgatcattg acgatccagt
    17281 tgccggaacg ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta
    17341 aactaagtgg tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaacag
    17401 cacgccacca caagccacca tcccgccgat caacgcggaa aggatcacaa taatcgcctt
    17461 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    17521 attcacatcg ttgacatgtg cttttgttaa ttgctcgaca agatactgcc gattacgtaa
    17581 ttcaccgttc agttccgcaa cgtcagtgac cgctttttct atttccagtt tgcgttcaat
    17641 accgtctgca ccgagagaaa tagaaaaatc ggggtcatct ttaacggcct gaccattact
    17701 gtaaacgggc tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg
    17761 aatgtttgca tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc
    17821 aaactgggtt ttgatctcca gtttattacg gacgttttct agcgactctt tcaccaccaa
    17881 cgtagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc
    17941 ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt
    18001 gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca aatccagttc
    18061 gtcgattttc gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc
    18121 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    18181 tttgatatcc agatccagca cacgaagctt agtgaatgtt ttctccaact cttgccactg
    18241 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    18301 gatcagcaag cctgcgcagg caaacgcaaa aacgaccgcc atgaccgttt ttttggcccg
    18361 ccataaaact gagattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    18421 ataatcggga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    18481 cctgaaaatg agtccaatag ggggaaatct ccacggcgct cactttacca gttggtgaga
    18541 attatccgaa gctgaagttt gtaaattcct tccaatgaat gcaacattca ctctatttaa
    18601 gagaaataac cagcaaactt ataaattaaa caaatgatta acccataatt atttcctgta
    18661 attatgggtt aatagataat gttaatttac ctgtttagcg ttgcgcgaat aatcggccca
    18721 attttttcaa acgcccctgg agagataata tccacatgcg cacaatcctg acgatagata
    18781 tccagttccg ctatccacgg cgaccaggcg cgttcgggac tcataccttc ctgaagtgtg
    18841 cgttcagcaa caaacagcgt cgctttaccg tcaaacggta cgctatgagc agtcgtcagc
    18901 aggcgcacag catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt
    18961 ccctgctgtg ctgccaggaa ggcctcgcgt tcgcggttaa tctccgccag cacttccggg
    19021 tccagaccat tagcttcttt ttcctgccag ttttgcgttt ctggcggcca ggtatccagc
    19081 aagccaagaa atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat accctgcgcc
    19141 agcgtaccgc caagggaata ccccagcagg taataagggc catgcggttg ttgttcaagt
    19201 aacgttgcca gatgcgcttc gcagacttca tccaggtttg ccgccgtctg catggggcca
    19261 ttggggcgcg gtgactgaat gccgataatc gaccattgtg gatcgagata acgcgagagc
    19321 acgctgaact gccaggcaaa accggacgca ggatggaaac aaaacagcgt cgggccatta
    19381 ccttcacgca acggcagaat ggtttcgaat cccatacgcc gggtgctgtc ttcttcagca
    19441 tcaataatcg ttgccagttt ggcgacagtt gacgcgacca tcacttgccc cggcgtcacc
    19501 tggcgggcaa cctgccgact taactgcgct gccagtttca ttgccagtag cgaatgaccg
    19561 ccaagcgcga agaaatcagc atcggcatcc tgcacgtcac accccagcaa cgacgagaat
    19621 gccgcggcga taatcgtttc actgcccgct ttcggcgcac gccctggcgc ttgtgccttc
    19681 agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag tggtaactgt
    19741 ggaagttgca gcagaaccac cggtaccata tgtggtggca atgtttcacg aagctgcgcc
    19801 tgcaatgcgc tggtatccaa cggcaggccc gattgcgaca ccagatagcc caccaattga
    19861 cgcgcatcac caccggtggc agccgcctgg ttaatcacac aggcgtgggt aacggcttgt
    19921 tcgacatccg gcagcgcctg catcacgcga tcgatttcgc ccagttcaat acgctgcccg
    19981 cgaattttta gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag
    20041 cgggcaacgt ctccggtacg gtacatccgt tcaccaggag caaaaggatc ggcaataaag
    20101 cggctggcgg tcagatcggg tcgtccaaga tacccctgcg ccagttgaat accggtgaga
    20161 tagagatctc ccgccacacc cggcggcacc ggatgcatca tcgcatcaag aatacgcagg
    20221 cccgtgttcc acaccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc
    20281 tcgccaaaag ccggatacca gctgacatct accgccgctt ccgtcgggcc atatagatta
    20341 tgcaacggcg cgccagttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca
    20401 ccactacaga aaacctgttt caacgtcgcg caactctggc gagcggtttg cggcgtcagc
    20461 gaggcaacaa atgccgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg
    20521 gcaaagaatt gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc catcaccagt
    20581 tttgcccctg cgataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc
    20641 gttttttggg caacgacatc ttcgcctgta agcggataat gattttgcat ccaaagcagg
    20701 cggttgacga tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag
    20761 gtaaagatga tataagccgt gtgatgcggt tgtgaaagtt gcagcggcgc actgccctgc
    20821 ggtgtaagcg gggcgttata gcaaaggttg gttaaatctg gaacatcggc aaagcgcggc
    20881 agttgatcgt cggtggtgat taacagcgac ggacgcgcat cttctagcat cattttcagg
    20941 cgatcgtccg gataaccggt atccagcggt agccaggccg cacctgcttc aacaatcgca
    21001 tgtagcgcca gggtcaaaaa gaccgagcgc ggtagcgcca ccgctacgct gtcgcccggt
    21061 ttaacgccgc gctcacgcag cagattcgcc agcgccacca cctgctcacg catttcccga
    21121 tagctgaact ggtaatgcgc atctgccagc gccggagcat ccggagtttt tgccgcttgt
    21181 tctgccacca gagcgctaag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc
    21241 gccagctgcg cgtattcacc tggcagcata atatcgacat caccgcacaa cagcgccgga
    21301 tctgcggcga actgggcaat cagcattttc aggcgttcag catgctggat taacgttggc
    21361 tcagcgtaac gctgtttatt ggcgaggatc tcaatactca aatcaccgtg tacatccggg
    21421 aacagggcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgcctga
    21481 acatcaggaa tatccagttg gtaatcaaat accttgatat tgagtaccgg accaaacagc
    21541 ggttcatcac ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga
    21601 tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct
    21661 tgcgccgcaa tgtgaatacc caacggcaaa acgttgagca cgggtccggt agccgtcagc
    21721 gccgccgagc ccagtcgacg cataaagata aatccggcgg cgtagtccat acgattgcac
    21781 aatcgcccca gccacaaggc tgccagcgca agggctaaat cggtacgctg cacacctgaa
    21841 agttgcgtag ccagctggcg gaattccccg tcggtaaatt ccagtttcag gcgcagaata
    21901 tctgccgagg cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg cggcggcagt
    21961 tgacgacgct gttctgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat
    22021 tgctggtact cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg
    22081 ccacgcagcc atgtgcagta aatattggcg atctggcgag taatggccgg gaaactgaag
    22141 ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc cgcaacatgt
    22201 atcagctgat gaaagaccag tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc
    22261 gccagcatta atgcctgcgc agtaccgtgc ggatcaatat tggttcgcag gtcgataatt
    22321 tctggcagtt cgaatatcag cgcatcatcg acccactgcc agacttcgcc gttatcttcc
    22381 gtaaaacgca tccgcaacgt atctgcttgc gctagtccga caaccaccgc gcgggccagt
    22441 aatggcgcat caacatctcc agttaactca acgtaatgcg ccacgctcca ggcggagggt
    22501 aaatccgaca gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc
    22561 tggctcattg tgcctcctgc aactgggtga aattggccgg tgtcagagta cgccagtgga
    22621 cttccagcca ctgctggcag gacgcctgtg actgcggctg acacacaatg tcccagcctg
    22681 ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt
    22741 aaaacgctcc ctgcggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg
    22801 gaaaagtggt tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc aacaaagcgc
    22861 atcatgtccg ccgtcaacct gacgccagaa aatggattct tttatggggt gtaattgcgc
    22921 atacagcgcc tgattggccc gcattatcat cggctcgcga atacccgctt ccagcacaat
    22981 gcgcagacct tcggcactaa cttgaccagc tttaagttgc tcaagtaaaa caccttcttg
    23041 ctgcccgccc cgatgcggcc accagtacga tcctgactgg cttaatacac agccaaagcg
    23101 ttcaggccag tgcagtccgg catacagcgc ggaaagtcca ccaaaactct gcccggcgac
    23161 cacggtgcga tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg gtaataactc
    23221 ttgctgtact gccagccaga aatccgcatt acacggcagt tcgtgagcgc ggtgcgtagt
    23281 gtcgatagcg tcgatcaaca catacacggc gggaggaagt tgctgacgat gggtcaacga
    23341 agtcagcaca ggccagacag gcatactctg cgcccaaaat tcgccatcga gcaaaactgc
    23401 cagcgggcgt tctcctgctg ttgcatcgcc agtggtaaaa atccatacac ggcgtgaatt
    23461 tttcaaccgt tcacttttcc agataatttc tttggcaggc gtttctggcg cttgcggaca
    23521 atcccatccc ggttgcagag gcgcttgcgg catttcgagt gcagaaacag cgtgccctcg
    23581 cccgcctttc cagctttgtg gattcagcgg atcggctatt gcctggggta atagttttcg
    23641 ccagccttca cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc
    23701 ggtgggaata aaacagtagc tgccgcgcca gttggcattg agttgtgtcg tccactgcca
    23761 gacgttggtg cctgcaattc gctgcatcga ctggggctgg ctgttctgat ggtgatcggt
    23821 cacaccagtg atgtagaccc atacgcgctt tatcgtcgag tattcttcag aaccttgggg
    23881 atcacgccac cagaaagtga cctcaaacat ttcgtcattc agacgctgcc attccgggcc
    23941 atgtttcgac tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca
    24001 tgtttactgt gcaatttttc attgattgca gaaatatatt gataatatta ttgataacta
    24061 tttgcatttg caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaaccaact
    24121 gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtattgatga
    24181 cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga
    24241 agattcattc cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag
    24301 agccgaccga tactcctgtt tcacatgacg atactattgt cgttaccgcc gccgagcaga
    24361 acttgcaggc acctggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccagttg
    24421 cccgcgatgt gtcggagatc atccgtacca tgccaggcgt taacctgacc ggtaactcca
    24481 ccagtggtca gcgtggtaat aaccgccaga ttgatattcg cggcatgggt ccggaaaaca
    24541 cgctgatttt gattgacggc aagccagtaa gcagccgtaa ttcggtgcgt cagggctggc
    24601 gtggtgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg attgaacgta
    24661 ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg
    24721 ttaacatcat taccaaaaaa ggcagtggtg agtggcacgg ctcctgggat gcttatttca
    24781 atgcgccaga acataaagag gaaggtgcca ccaaacgtac caacttcagc ctgaccggtc
    24841 cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg
    24901 cgtgggatat caaccagggc catcagtccg cgcgtgccgg aacgtatgtc acgacgttac
    24961 cagccgggcg cgaaggggtg atcaacaaag atattaatgg cgtggtgcgc tgggacttcg
    25021 caccattgca atcgctggaa ctggaagcgg gttacagccg ccagggtaac ctgtatgcgg
    25081 gcgataccca gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca
    25141 accgcctgta tcgccagaac tacgcgctga cctggaacgg tggctgggat aacggcgtga
    25201 ccaccagcaa ctgggtgcag tatgaacaca cccgtaactc gcgtattccg gaaggtctgg
    25261 cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc
    25321 ttgatgacgt gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaga
    25381 cgctgacgct gggtacggag tggaatcagc aacggatgaa ggacttgagt tccaataccc
    25441 aggcgctgac cggggcgaac accggcggcg ctattgatgg tgtgagtgcc accgaccgta
    25501 gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaacatg gagctgactg
    25561 acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact
    25621 ggagcccggc gctgaacata tcgcaaggtt taggcgatga cttcacgctc aaaatgggca
    25681 ttgcccgtgc ttataaagca ccgagcctgt accagactaa cccgaactac attctctaca
    25741 gtaaaggtca gggttgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc
    25801 tgaaagcaga aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacggctggc
    25861 tggcgggcgt cacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtgg
    25921 ctgtaggaca aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcag
    25981 tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacca
    26041 ataacatcac ttatatgctg aagagtaaaa acaaaaccac gggcgaccgt ttgtcgatca
    26101 tcccggagta tacgttgaac tcaacgctga gctggcaggc acgagaagat ttgtcgatgc
    26161 aaacgacctt cacctggtac ggtaagcagc agccgaagaa gtacaactat aaaggtcagc
    26221 cagcggttgg cccggaaacc aaagaaatca gtccttacag cattgtgggt ctgagcgcga
    26281 cctgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac
    26341 gtttgtggcg tgcgggtaat gcccagacca cgggcgatct ggcaggggcc aactatatcg
    26401 cgggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtacatg agcgtaaaca
    26461 ctcacttctg atgctaacgt cagattgttg acaaagtgcg cgtcgttcat gccggatgcg
    26521 gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccatg
    26581 taggcctgat aagcgtagcg catcaggcag tttagcgttt gtcatcagtc tctaatatgg
    26641 tcgacatgaa aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg
    26701 agttcgatcc ggcgaatttt tgtgagcagg atttactctg gctgccgcat tacgcgcaac
    26761 tgcaacacgc tggacggaag cgcaaagccg aacatttagc cgggcggatc gctgcaattt
    26821 atgcgctgcg ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaaccgg
    26881 tgtggcctgc ggaggtatac ggcagtatta gccactgcgg ggctacggca ttggccgtgg
    26941 tttcccgtca accgattggc gttgatatcg aagaaatttt ttctgcgcaa acagcgaccg
    27001 aattgacaga caacattatt acaccagcag aacacgagcg actcgcagac tgcggtttag
    27061 ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg
    27121 agatccaaac tgatgcaggt tttctggact atcagataat tagctggaat aaacagcagg
    27181 tcatcattca tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaaaatag
    27241 tcataactct gtgccaacac gattaattga caacatctgg tacgattcgc ccgcagccat
    27301 cactgaccgc gggcgaaagt gtaaagcagg tgccttacca ctctgacctg acaaccggat
    27361 atgcggggat tgctccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt
    27421 aaactcaata ttacgttcct ttacggtgaa ctcacacagc gagtctccga ccagaagcgt
    27481 aaatcccagt accgtcaaac acagcactat gactgccaca agggcatatt tcgtcagcat
    27541 gtttatattg cctccatgcg aagaagggac taccatccga gttgttgagg ctcggagtgg
    27601 cagccccagg attgatagaa atatctcctg gggcttcatc tttccagacc tcagagttaa
    27661 cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttcctcaa caaaatagct
    27721 gtttatttat acagtgaatc ctgtttcgga cttctgtcgg caaaactcat cattgctctt
    27781 aaaacgaaat cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc aggtgcctta
    27841 ccatcctgac ctgacaaccg gatatgcggg gattgctccc cgcacaacgg ctacttcttc
    27901 ggttcgtaag cgagaacaac cttaaactca atattacgtt cctttacggt gaactcacac
    27961 agcgaatccc cgaccagaag cgtaaatccc agcaccgtta aacacagcac tatgactgcc
    28021 gcaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc
    28081 caaattgttc aggtttgggt aggtagcccc agggttgata gaaatatcgt cctggggctt
    28141 catctttcca gacctcagag taaacctgaa accggaaagc ttcaggcacc cgcagttatc
    28201 ttacccttgc ttagcaaaat agctgtttat ttatacagta agtcctgttt cggacttctc
    28261 actccccatt tgatcaactg aagctgaacc cgtttttttc tgcatgcctc aatcgtcaaa
    28321 attcttcgcc accagccagc cgtgccataa cttgtcacat tgcaaaagta gccaaattgc
    28381 ttcagtcacc atcaccaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct
    28441 ccgacttcta tactgaatag aaaacgccaa cttaagagaa acctatgcca ttacccgatt
    28501 ttcatgtttc tgaacctttt accctcggta ttgaactgga aatgcaggtg gttaatccgc
    28561 cgggctatga cttaagccag gactcttcaa tgctgattga cgcggttaaa aatgagatca
    28621 cggccggaga ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt
    28681 gccgtgatat caaccaggct gccgggcagt tttcagcgat gcagaaagtc gtattgcagg
    28741 cagccgcaga ccatcatctg gaaatttgcg gcggtggcac gcacccgttt cagaaatggc
    28801 agcgtcagga ggtatgcgac aacgaacgct atcaacgaac gctggaaaac tttggctatc
    28861 tcatccagca ggcgaccgtt tttggtcagc atgtccatgt tggctgcgcc agtggcgatg
    28921 acgccattta tttgctgcac ggcctgtcac ggtttgtgcc gcacttcatc gccctttccg
    28981 ccgcgtcgcc gtatatgcag ggaacggata cgcgttttgc ctcctcacga ccgaatattt
    29041 tttccgcctt tcccgataat ggcccgatgc cgtgggtcag taactggcaa caatttgaag
    29101 ccctgtttcg ctgcctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg
    29161 atattcgccc cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa
    29221 cccttagcca cgcggtaaat atggcgggat taattcaggc caccgcccac tggttactga
    29281 cggaacgccc gtttaaacat caggagaaag attacctgct gtataaattc aaccgtttcc
    29341 aggcctgccg ctatgggctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac
    29401 cgctaacgga agataccttg cgattgctgg aaaaaatcgc cccttccgca cataaaatgg
    29461 gtgcatcgag cgcgattgag gccctgcatc gccaggtcgt cagcggtctg aatgaagcgc
    29521 agctgatgcg cgatttcgtc gccgatggtg gctcgctgat tgggctggtg aaaaagcatt
    29581 gtgagatctg ggccggtgac taagtaggga attgccttac agaagaatat ccctgacaat
    29641 ggtgttttaa cttacattta acaatgttat gaaacaccct ttagaaacct tgactaccgc
    29701 agcaggcatt ttgctgatgg ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg
    29761 gctgacgctg gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta
    29821 cactttattg ttttgtctgt ggtttttagt gctgggcgct attgagtatt ttgttctgcg
    29881 ctttatctgg cgacgctggt tctcgctggc ggattaaacg tcgattatcg ccgtacttcg
    29941 cggcatactt tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc
    30001 acgaaacggc gaaacgcctg gcccttgagt taccctttgt cgagctttgc tggccttttg
    30061 gcccggagtt cgatgttttt aaaattggcg gcaaaatttt tatgctgtcg tcggagctgc
    30121 gcggcgtccc ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaga
    30181 tatacccgag cattaagcca gggtatcaca tgaataaaaa gcactggatt tcggtgtatc
    30241 ccggcgagga aatctccgaa gcgttaattc gcgatctgat caacgattcg tggaatctgg
    30301 tggttgatgg tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcggaaatc
    30361 tatagcgcat ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa
    30421 aattactttc acatggagtc tttatggata tcatttctgt cgccttaaag cgtcattcca
    30481 ctaaggcatt tgatgccagt aaaaaactta ccccggaaca ggccgagcag atcaaaactc
    30541 tcctgcaata cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca
    30601 cggaagaagg taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacgaac
    30661 gtaaaatact tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg
    30721 cctggctgaa gctggttgtt gaccaggaag atgctgatgg ccgctttgcc acaccggaag
    30781 cgaaagccgc gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt aaagatctgc
    30841 atgatgatgc agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg
    30901 gcgtggcggc gctgggtctg gacgcggtac ccatcgaagg ttttgacgcc gccatcctcg
    30961 atgcagaatt tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc
    31021 atcacagcgt tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacatca
    31081 ccttaaccga agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt
    31141 ctcctgattt gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga
    31201 cgttatgcag gatttaatat cccaggttga agatttagcg ggtattgaga tcgatcacac
    31261 cacctcgatg gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat
    31321 tttgcattgg gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg
    31381 gttgcaaatc attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat
    31441 tatcgtcaat attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct
    31501 gactacctgc gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct
    31561 ggatgttatt ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg
    31621 gatatttcag gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct
    31681 gctgattggt cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct
    31741 gatgttggtt tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa
    31801 cgatatgctg aaactgggcg actggctgga gatgccgaaa tacggcgcgg atggcgcggt
    31861 gatcgatatt gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat
    31921 tcccacctgg tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg
    31981 cgggcgacgt attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga
    32041 agacgaaatg caacgtctga ataaagcgca cttgttaaaa ccttatttaa ccagccgcca
    32101 tcaggaaatt aatgagtgga atcgccagca aggttctacg gagtcgatat taaacctgcg
    32161 ccgaatgacc aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc
    32221 gcggattcgt aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt
    32281 accgctcgag atctacgcgt ttactaacac cgtggtgtgg ctggaatatg aaagcattca
    32341 ggctgatata ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca
    32401 gtcgccaacc ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa
    32461 accgctctca tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct
    32521 tatttccgac gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg
    32581 atcgctgaca agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag
    32641 gcaatgcaaa gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc
    32701 tgacgtcgca tcgctgcacg aaaacgcaga tgcgccagac agatcataat ccagttcaac
    32761 agcagcgttg ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag
    32821 ttgattaata ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcaca
    32881 ccgcgacggc tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac
    32941 agcatgcggc tgttagagta aaccccgctg ttatacactg acagcgatgc taccagaatg
    33001 acgaagttca gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaac
    33061 gggctactgt tggatttcac ttccacccac ggatagagcg ccagtaaaac caccagtgaa
    33121 ccgatgtaaa acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt
    33181 tccggatcgc gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac
    33241 ataattaccg ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag
    33301 cgccagaggt tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg
    33361 ccaaagccga tcataccgat gatcgccagc actttaatca gcgcaaacca gaactcggtt
    33421 tcaccatata agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca
    33481 gcccaaatcc acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc
    33541 agctctgcca ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc
    33601 gcaaacggtc cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcctca
    33661 accaccattt cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag
    33721 cccagcaata cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca
    33781 gtaccaattg cgccacccaa cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga
    33841 agcgtcggct cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct
    33901 ttcccctgtg tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca
    33961 agacacaatc cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt
    34021 gcagccacat cagcttatac gccgccggga taataaacag cgacagcaaa ggtgcggtga
    34081 tcatgccgcc aatcatcggt gcggcaatcc ggctcatcac ctctgaacca gcccccgttc
    34141 cccacagaat cggcagcaga ccggcgataa tcaccgccac cgtcatcgct ttcgggcgca
    34201 cgcgcaggac cgcgccgtga tataacgcct catccagctt ctgctcgctg aatgtttgtg
    34261 gattattcaa cgacggcacg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc
    34321 caaattcggc ggcgaccccg gcgagggcga taaagccagt gcccgtcgcc acggaaagat
    34381 gaaagcccat ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga
    34441 taatcagcaa cgcttcgccc acccgacgga acgccagata caacagcacg aagataatca
    34501 tcaacgtcat cggcaccatg agtttaagct tatggttggc gcgctccagt agctcgaact
    34561 gcccggagaa tgccacgctg gtgcccggtt ttaactgcac tttttcagct atcgcttttt
    34621 gcaaatcgtg aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg
    34681 tcgggcgcgc attctcggtt ttcagcatcg acggtccggt agagacttta atgtcggcca
    34741 cgtctgccag ggtgatttgc tgcttcatcg gcgtcaggat cggcagctgg cgcagtgcct
    34801 gcggactatc gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt
    34861 ccaccgtttc gccaaccatc gccccgccca ccgcagaagt cacaaacaac tgcacatccg
    34921 ccaccgtcat accgtaacgt gcggcttttt cacggttaat ctcaacgttg atatagcgcc
    34981 caccttccag ccgctcggca agcgcagaag ctacgcctgg caccgttcgc gctacttctt
    35041 caatttgctc agccatcgcg tcgatatccg ccagcacagt gccggaaact ttaatgccga
    35101 tggggctttt aatgccggtt gagagcatat cgatacggtt acgaattggc ggcacccaca
    35161 gattcgccag ccccggcagc cgcacggtgt tatccagttc ctcaatgatt ttgtccatcg
    35221 tcatgcctgg ccgccactgc tcctgcggct taagctggat ggtcgtttct accatctcca
    35281 gcggagcaga atcggtggcg gtttccgctt tcccggtttt gccaaatacc cgcgccactt
    35341 caggtacgct cataattagc ttgtcggttt tttgcagcat actcgccgcc tctgctgcgg
    35401 aaatccccgg cagcgtcgat ggcatataca acaagtcgcc ttcattgatc tgcggtaaaa
    35461 attccccgcc aactttattg agcggccaga gaaccgtcag caccgaaagc gccgccacca
    35521 gcagcgtggt tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca
    35581 aaaagcgatt gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtagccca
    35641 tcaggatcgg gatcactacg atcgccagca gcgccgcacc cgccatcgca tacgttttgg
    35701 tgaacgccaa cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa
    35761 acgacaacgt gataatcagc agactgataa atagcgccgg ccccacttca acagacgcat
    35821 cggtgatcac ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc
    35881 actcttccag ccgtttatgt gcattttcga tcatgacgat agcggcatcg accatcgccc
    35941 cgacggcaat cgcaatgcca cccaacgaca taatattggc attcagtccc tggaagtgca
    36001 tgacaataaa agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc
    36061 gcacatgcca gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact
    36121 tgccgctgag gttgtcgatg gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta
    36181 tctccacgcc ttccggcaga ctacttttca gcgtttccag tttgtccttc acggcggcga
    36241 tcacttctcg ggcgtttttg ccggatcgca ggatcaccac cccgcccgcc acttcgcctt
    36301 cgccgtttag ttcggcaatg ccccggcgca tctccgggcc aatctggacc ttcgcaacat
    36361 cgcgcagata aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt
    36421 cgagcgtttg cagatagccg ctggcgcgca ccatatattc cgcttccgcc agttcgatcg
    36481 acgaaccgcc cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga
    36541 tgccatactg cgccaggcgc tggggatcga taaccacctg atactctttc accacaccgc
    36601 ccaccgacgc cacttccgca acgtcaggga tggttttcag ctcatatttg agaaaccagt
    36661 cctgtaatga gcgtaaatcg gccagatcgt gcttaccgct gcgatccacc agtgcatatt
    36721 catagatcca gccaacaccc gtggcatctg gccccagctc ggcgctgact cccgcaggca
    36781 gcttaccctg tacctggttg aggtactcca gcacccgcga acgcgcccag tacggatcgg
    36841 tgccatcttc gaaaatgaca tacacataag agtcgccaaa ctgtgagaaa ccgcgcacag
    36901 tcttcgcgcc aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa
    36961 cgatttgtgg tgcctgaccg ggatagctgg ttttaataat aacctgcaca tcggagagat
    37021 ccggcagcgc atccactggc gtattaatga tggtccaggt gccccagatg ctcagaaaca
    37081 acgcgcccat cagcaccaaa aaacggttcg ccaccgagcg acgaataatc cattcaatca
    37141 ttggttattc cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc
    37201 agaaatattg gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc
    37261 cgccagacca gagcgtaatg cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt
    37321 cggtacaaag cgcccgtcgg catcaacggt aatcacccgc tgttcgctgc cggtatcaat
    37381 cagcgcttgt gacggaatga gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc
    37441 gttcatgccc ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt
    37501 gcgggtcgcg gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc
    37561 cggtcgcgcc ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgataga
    37621 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattttcg ctaccacgtt
    37681 atctttggcg atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg
    37741 cgctttgagc gtaaagcgag tctggatttt ttgcgtggcg atcaggcggc gaatatccgc
    37801 ctccggcatt cccgccagtc gcagccgctc aagaatgcct tcagtctggg tcgccgtacc
    37861 gccggtttcg cgcagcagta aatactcact ctgcgcttcc acccagtcag gaatggtcag
    37921 gtcgagaagc ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttgtc
    37981 gataaacccg gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt
    38041 cgccgggaaa ctctgggcaa aagtcagcgg cccgcgcgtg acggtagccg ttttcacccc
    38101 cagattctgc gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc
    38161 atatttcggc accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt
    38221 attggggtac atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc
    38281 tgcgggtggt tcagcctttg caaaccaggt aaaacctgcc gcagaaataa taccgcccgc
    38341 gatcatgctg ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct
    38401 gactttaata tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt
    38461 gtcgccagtt ttaatttcac tcattttcgt ctgcggagtg atggtaaagc gcatggtcat
    38521 ctccggccag ttcacggcag caatcggatc gtgatggatg gtgatttttt tgctttccag
    38581 atcaataccc tttaccacgc cagtggcgct aataacctgc ggttgtactt cgctcatggt
    38641 ttcatgatga tgttcgttag cctgggcatt aaagccaata acggtaaaca gactgaacat
    38701 tgcgacttgc agtgcttttt tcatttattc tctcctggag ttaaaaatta ttgctgccaa
    38761 ccgcccccta aggcggtata caaagaaatt tcgttaacct gacgggcata attcaggtcg
    38821 agtaaagttt gtcgggttgc aaataaagaa cgctcggcat ccagcacttc cagataactt
    38881 accgcgccgt gctgatataa tgcccgcgcc cgttgcaaag taatttgcag cgacgccaga
    38941 taacgctgct gggcgctgat ttgatcgttc aggctttgac gtaatgccag cgcatctgcc
    39001 acttctttaa aggcgttctg gattttctgt tcataattca ctaccgactg ctgctggcga
    39061 atttcggcga tatccagatt ggcttggttg cgtccggcat taaaaatggg gatctcaatt
    39121 ttaggaataa aattccacat cccgctgcta gcgttaaata atgaagacag atcgctgctg
    39181 gcggttgata ttccgctggt caggctgata gacgggaaaa atgccgcacg cgcagcgcca
    39241 atattggcat tagccgccat taacgcgtgt tcagcttcca taatatccgg gcgctgcaat
    39301 aagatttgcg acgacaatcc cgccggtaat ttaacgcttt gcaggctgtc gctgtttact
    39361 gtctgcgctt gcggcagctt gccgtagctt cccaataaca gttgcaatgc attattcgcc
    39421 tgcgccagtt ccccctgacg tttagcgatg tcgctgcggg tactttctat caccccgcga
    39481 gcctgttcca gcgccagaac attactgcta cctgtcaaca gttgtttttc gacaaacgca
    39541 tatgactgct gataattacg cagcgtttct tcggctattt gcaattgcgc atacgccagt
    39601 tgctgattga aatagctttg cgcgacatta gaaaccagca gaatatgcac cgcgcgctga
    39661 gcttcctcag tggctaaata attttgtcgc tcggcttcgc tcatgttctt taagcgaccg
    39721 aaaaaatcga ggtcaaagct ggcgttaagg ccggtcgaaa actcccgtgt cgtggcggta
    39781 ttccctttaa gattaccgct ccagctgccg ctgccctcgc cattgagctg tgggtagcgg
    39841 tcggcatcgg tcagacgata ttgcgcccgt gcctcctgca ctttcagcgt cgccatgcgc
    39901 aaatcccgat tattcaccag cgcctcgcta atcagcgtct tcacctgatt atcaacaaaa
    39961 aaggtgcgcc agcccgcgtt ctgatagtta tctgctgcgt taaccaggcc gttctggctg
    40021 agtgagaact gctgcggcac gggcattgcc ggacgctgat aatccggtgc cagtgaacaa
    40081 ccggttagcg caagggccac acaaaatggc agaagtttac aaggagacat aggctcataa
    40141 tttctggtga ttttatgccg ccaactttac tcgccaggct ctgattttcc ggtgacagaa
    40201 aaatgacaaa attgtcattt tgccaataag cgattgccat ctgatcccgc tactctagaa
    40261 ttgcccgggc aacatgcgga ggaaatatga aactgttgat tgtcgaagat gaaaagaaaa
    40321 ccggagaata cttgaccaaa gggttaaccg aagccggttt tgtggtcgat ttggccgaca
    40381 acgggttgaa tggctaccat ctggcgatga ccggtgatta tgatctgata atcctcgata
    40441 ttatgctgcc ggacgtgaac ggctgggata tcgtgcgcat gttgcgttcc gccaataaag
    40501 ggatgccgat tctgttgctt accgcgcttg gcaccattga acatcgcgtc aaggggctgg
    40561 agttgggggc ggatgactat ctggtgaagc cgttcgcttt tgctgaactg ctggcgcggg
    40621 tgcgcactct cctgcggcgc ggggcggcgg tgattatcga aagtcagttt caggttgccg
    40681 atctgatggt cgatctcgtc agccgcaaag tcacccgcag cggcacgcgc atcactctga
    40741 ccagtaaaga gtttactctg ctggagtttt tcctccgcca tcagggcgaa gtgctgcccc
    40801 gctcgcttat cgcctcgcag gtgtgggaca tgaatttcga cagcgacact aacgccattg
    40861 atgtagcggt gaagcggctg cgcggcaaaa tcgacaacga ctttgagcca aagctaattc
    40921 agaccgtgcg cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa gccatttcag
    40981 cgcccgtttt cgctggcaac ccgcctgacc ttttttatca gcctggccac catcgcagcg
    41041 tttttcgcct ttgcatggat catgatccac tcagtaaaag tgcattttgc cgagcaggat
    41101 attaatgatt taaaagaaat tagcgccacg cttgaacggg tacttaatca tcctgacgag
    41161 acgcaagccc gacgcttaat gacgctggaa gatatcgtca gtggttattc caacgtgttg
    41221 atctccctgg cagatagcca tggtaaaacg gtgtatcact cccccggtgc gccggatatc
    41281 cgcgagttta cgcgtgacgc catacccgat aaagacgcgc ggggcggcga ggtgtatctc
    41341 ctttccggcc cgacgattat gatgccaggg catggtcacg ggcatatgga acacagcaac
    41401 tggcggatga ttaacttgtc ggttggcccg ttggtggacg gcaaaccgat ttatacgctc
    41461 tacatcgcgc tttcgatcga ttttcatctt cattacataa atgatttgat gaataaactt
    41521 attatgaccg catcgatcat cagcatcctg atcgtcttta tcgtgctatt ggcggtacat
    41581 aaaggtcacg cgccgatccg cagcgtcagc cgtcaaatcc agaatattac ctcgaaagat
    41641 ctcgacgttc gccttgaccc gcagaccgtg cccattgagc tggaacagct ggtactgtcg
    41701 ttcaaccata tgatcgagcg tattgaggat gtctttaccc gccagtccaa tttctcagcg
    41761 gatatcgctc acgaaattcg cacgccgatt accaatctca tcacgcaaac ggaaatcgcc
    41821 ctcagccagt ctcgcagcca gaaggagctg gaagatgtgc tctactctaa tctcgaagag
    41881 ctgacgcgaa tggcgaaaat ggtcagcgat atgctgtttc tcgctcaggc cgataacaac
    41941 cagctaatcc ccgaaaagaa aatgctcaac ctggcggatg aagtcggcaa agtgttcgat
    42001 tttttcgagg cgttagcgga agatcgcggc gtggagttgc gatttgttgg cgacgaatgt
    42061 caggttgcgg gcgatccgct gatgctgcgt cgggcgttaa gcaacctgct ttctaatgcc
    42121 ctgcgttata cgccgcccgg agaggcaatt gtagtgcgct gccagacggt cgatcaccag
    42181 gtgcaagtta ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt accgcgattg
    42241 tttgaccgtt tctaccgcgt tgacccttcc cgccagcgaa aaggcgaagg tagcggcatt
    42301 gggctggcga tagtgaaatc gattgttgtc gcgcataaag gcacggttgc agtaacgtca
    42361 gatgcgcggg ggacaaggtt tgtgattatg ctgccggaga gagagtgatt attaagttca
    42421 aattcagggc gtggtgcatc acctcctggt attgttgatc aacaaattag agaaaaactt
    42481 attaaacata tgaaatatcg aaggctttta tttttcaaag tttcacgaga tgtttgtata
    42541 tgaaacttag ttaaatctgt gtttcttcca attgtagatc acaatgccct ttctaaattt
    42601 tcatcgt
//
LOCUS       DAZGCS010000034        41682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGCS010000034 DAZGCS010000000
VERSION     DAZGCS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.034"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(120..196)
                     /locus_tag="ACHK01_001881"
     tRNA            complement(120..196)
                     /locus_tag="ACHK01_001881"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Met,seq:cat)
     gene            405..1502
                     /gene="mltA"
                     /locus_tag="ACHK01_001882"
     CDS             405..1502
                     /gene="mltA"
                     /locus_tag="ACHK01_001882"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO8096842.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1579..2385
                     /gene="tcdA"
                     /locus_tag="ACHK01_001883"
     CDS             1579..2385
                     /gene="tcdA"
                     /locus_tag="ACHK01_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO8096843.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2436..2879)
                     /gene="csdE"
                     /locus_tag="ACHK01_001884"
     CDS             complement(2436..2879)
                     /gene="csdE"
                     /locus_tag="ACHK01_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO8096844.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2879..4084)
                     /gene="csdA"
                     /locus_tag="ACHK01_001885"
     CDS             complement(2879..4084)
                     /gene="csdA"
                     /locus_tag="ACHK01_001885"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO8096845.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4276..4503
                     /gene="ygdI"
                     /locus_tag="ACHK01_001886"
     CDS             4276..4503
                     /gene="ygdI"
                     /locus_tag="ACHK01_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO8096846.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4854..5771
                     /gene="gcvA"
                     /locus_tag="ACHK01_001887"
     CDS             4854..5771
                     /gene="gcvA"
                     /locus_tag="ACHK01_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO8096847.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5790..6185
                     /gene="ygdD"
                     /locus_tag="ACHK01_001888"
     CDS             5790..6185
                     /gene="ygdD"
                     /locus_tag="ACHK01_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO8096848.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6178..7278
                     /gene="rlmM"
                     /locus_tag="ACHK01_001889"
     CDS             6178..7278
                     /gene="rlmM"
                     /locus_tag="ACHK01_001889"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO8096849.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7322..8053)
                     /gene="fucR"
                     /locus_tag="ACHK01_001890"
     CDS             complement(7322..8053)
                     /gene="fucR"
                     /locus_tag="ACHK01_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO8096850.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8111..8533)
                     /gene="fucU"
                     /locus_tag="ACHK01_001891"
     CDS             complement(8111..8533)
                     /gene="fucU"
                     /locus_tag="ACHK01_001891"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO8096851.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8535..9953)
                     /gene="fucK"
                     /locus_tag="ACHK01_001892"
     CDS             complement(8535..9953)
                     /gene="fucK"
                     /locus_tag="ACHK01_001892"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO8096852.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10062..11837)
                     /gene="fucI"
                     /locus_tag="ACHK01_001893"
     CDS             complement(10062..11837)
                     /gene="fucI"
                     /locus_tag="ACHK01_001893"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO8096853.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11870..13186)
                     /gene="fucP"
                     /locus_tag="ACHK01_001894"
     CDS             complement(11870..13186)
                     /gene="fucP"
                     /locus_tag="ACHK01_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8096854.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13733..14380
                     /gene="fucA"
                     /locus_tag="ACHK01_001895"
     CDS             13733..14380
                     /gene="fucA"
                     /locus_tag="ACHK01_001895"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO8096855.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14408..15556
                     /gene="fucO"
                     /locus_tag="ACHK01_001896"
     CDS             14408..15556
                     /gene="fucO"
                     /locus_tag="ACHK01_001896"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO8096856.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15611..16366)
                     /gene="xni"
                     /locus_tag="ACHK01_001897"
     CDS             complement(15611..16366)
                     /gene="xni"
                     /locus_tag="ACHK01_001897"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO8096857.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16478..17845)
                     /gene="sdaB"
                     /locus_tag="ACHK01_001898"
     CDS             complement(16478..17845)
                     /gene="sdaB"
                     /locus_tag="ACHK01_001898"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO8096858.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17903..19192)
                     /gene="sdaC"
                     /locus_tag="ACHK01_001899"
     CDS             complement(17903..19192)
                     /gene="sdaC"
                     /locus_tag="ACHK01_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO8096859.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19749..21113)
                     /gene="ppnN"
                     /locus_tag="ACHK01_001900"
     CDS             complement(19749..21113)
                     /gene="ppnN"
                     /locus_tag="ACHK01_001900"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO8096860.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21225..22073)
                     /gene="queF"
                     /locus_tag="ACHK01_001901"
     CDS             complement(21225..22073)
                     /gene="queF"
                     /locus_tag="ACHK01_001901"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO8096861.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22141..22686
                     /gene="syd"
                     /locus_tag="ACHK01_001902"
     CDS             22141..22686
                     /gene="syd"
                     /locus_tag="ACHK01_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO8096862.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23308..23637
                     /gene="yqcC"
                     /locus_tag="ACHK01_001903"
     CDS             23308..23637
                     /gene="yqcC"
                     /locus_tag="ACHK01_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO8096863.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23637..24419
                     /gene="truC"
                     /locus_tag="ACHK01_001904"
     CDS             23637..24419
                     /gene="truC"
                     /locus_tag="ACHK01_001904"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO8096864.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24437..24886
                     /gene="yqcA"
                     /locus_tag="ACHK01_001905"
     CDS             24437..24886
                     /gene="yqcA"
                     /locus_tag="ACHK01_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO8096865.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25321..26673
                     /gene="gudP"
                     /locus_tag="ACHK01_001906"
     CDS             25321..26673
                     /gene="gudP"
                     /locus_tag="ACHK01_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO8096866.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26675..28015
                     /gene="gudX"
                     /locus_tag="ACHK01_001907"
     CDS             26675..28015
                     /gene="gudX"
                     /locus_tag="ACHK01_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO8096867.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28036..29376
                     /gene="gudD"
                     /locus_tag="ACHK01_001908"
     CDS             28036..29376
                     /gene="gudD"
                     /locus_tag="ACHK01_001908"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO8096868.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29608..32364)
                     /gene="barA"
                     /locus_tag="ACHK01_001909"
     CDS             complement(29608..32364)
                     /gene="barA"
                     /locus_tag="ACHK01_001909"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO8096869.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32421..33722
                     /gene="rlmD"
                     /locus_tag="ACHK01_001910"
     CDS             32421..33722
                     /gene="rlmD"
                     /locus_tag="ACHK01_001910"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO8096870.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33770..36004
                     /gene="relA"
                     /locus_tag="ACHK01_001911"
     CDS             33770..36004
                     /gene="relA"
                     /locus_tag="ACHK01_001911"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO8096871.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36082..36330
                     /gene="mazE"
                     /locus_tag="ACHK01_001912"
     CDS             36082..36330
                     /gene="mazE"
                     /locus_tag="ACHK01_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO8096872.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36330..36665
                     /gene="mazF"
                     /locus_tag="ACHK01_001913"
     CDS             36330..36665
                     /gene="mazF"
                     /locus_tag="ACHK01_001913"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO8096873.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36736..37527
                     /gene="mazG"
                     /locus_tag="ACHK01_001914"
     CDS             36736..37527
                     /gene="mazG"
                     /locus_tag="ACHK01_001914"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO8096874.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37755..39392
                     /gene="pyrG"
                     /locus_tag="ACHK01_001915"
     CDS             37755..39392
                     /gene="pyrG"
                     /locus_tag="ACHK01_001915"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO8096875.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPECKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39480..40778
                     /gene="eno"
                     /locus_tag="ACHK01_001916"
     CDS             39480..40778
                     /gene="eno"
                     /locus_tag="ACHK01_001916"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO8096876.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40838..41682)
                     /gene="ygcG"
                     /locus_tag="ACHK01_001917"
                     /pseudo
     CDS             complement(40838..41682)
                     /gene="ygcG"
                     /locus_tag="ACHK01_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT        10364 a        10078 c        11060 g        10180 t
ORIGIN      
        1 ttggggggga tcaacggttt actcattgtt tcaggcttca ctgaaacgga taaggaagcg
       61 taaaaacgaa aaaaccgcaa gctaaaggta gcgttgcggt ttctgatatt ctaataaatt
      121 ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag
      181 gctgctccac cccgcgtccg tggatgcgca ctatactccg ctcgcgtttt catgcaacct
      241 tttttttacc ccgatcacgg atttagcatg attttgaaca aaaacaaacc actaaagcct
      301 attttttgta caaaatttgc gtcagggaga ggctgctcat ttcattaaac gcggcggttt
      361 gttatcttcg ttgcgcctta ttttttaacc tgaagaagag aacaatgaaa ggacgttggg
      421 taaagtacct tcttatgggc acggttgtgg caatgcttgc cgcctgctct tccaaaccaa
      481 ccgatcgcgg acagcaatat aaagacggga aatttaccca gcctttctct ctggtgaacc
      541 agccagatgc cgttggcgcg ccgattaacg ccggtgattt tgccgagcaa attaaccata
      601 tccgtaattc gtcaccgcgt ctgtatggca accagagtaa tgtttataac gcggtgcaag
      661 agtggctgcg cgcaggcggt gatacccgca atatgcgcca gttcggcatt gatgcctggc
      721 agatggaagg tgccgacaac tatggtaacg tgcagtttac aggctattac acgccggtaa
      781 ttcaggcgcg ccatacccgc cagggcgagt tccagtatcc tatttaccgt atgccgccaa
      841 aacgtggtcg tctgccgtct cgtgcggaga tctacgcagg ggcgctgagt gataaatata
      901 ttctcgctta cagcaactcc ctgatggata acttcattat ggatgtgcag ggtagcgggt
      961 atatcgactt tggtgatggc agtccgctta actttttcag ctatgcaggg aaaaacggtc
     1021 atgcctatcg cagcattggt aaggtgctga tcgaccgtgg cgaagtgaaa aaagaagata
     1081 tgtcgatgca ggcgattcgt cattggggcg aaacacacag tgaagccgag gttcgcgagc
     1141 tgctggaaca gaacccgtct ttcgttttct ttaaaccgca atcttttgca ccggtgaaag
     1201 gggcaagtgc ggtgccgctg gttggccgtg cttctgttgc ctccgatcgt tcaattattc
     1261 cgccaggtac taccttgctg gcagaagtgc cgctgctgga taataacggc aaatttaacg
     1321 gtcagtacga attgcgtctg atggtggcgc tggatgtcgg tggcgcaatc aaaggccaac
     1381 acttcgatat ctatcaaggg atcgggccgg aagccggaca tcgcgcaggt tggtacaacc
     1441 actatggacg tgtctgggtg ctgaaaaccg ccccgggcgc aggtaacgtc tttagcggct
     1501 gatgtgttat tctgtatacc gtttttacgg atacgccagg gtgagggata acctcaccct
     1561 ttttaattct gaggtgctat gtctgtggta attagtgatg catggcgtca gcgttttggt
     1621 ggcacagcgc gtctgtatgg tgaaaaagcg ttgcaactgt ttgctgacgc gcatatttgt
     1681 gtggttggta tcggtggtgt cggttcctgg gcggcggaag cgctggcgcg cacggggatt
     1741 ggcgcaatca cgcttatcga tatggatgat gtgtgcgtca ccaataccaa tcggcaaatt
     1801 cacgctctgc gcgataacgt cgggctggca aaagcggaag tcatggcgga gcgtattcgc
     1861 cagattaacc cggagtgccg tgtaacggtg gtggatgatt tcgtgacgcc ggataacgta
     1921 gcgcagtata tgagcgtggg ttattcgtac gtgattgatg ccattgatag tgtacggccc
     1981 aaagcggcgc tgattgctta ttgtcggcgc aataaaatcc cgctggtcac gaccggtggc
     2041 gcgggtgggc agattgaccc gacgcagatt caggttaccg atctggcgaa aacgattcag
     2101 gatccgctgg cggcgaagtt gcgcgagcgc ctgaaaagcg attttggcgt agtgaaaaac
     2161 agtaaaggta agctcggcgt cgattgcgtg ttttctactg aagcgctggt gtacccgcag
     2221 tcagacggta ctgtgtgtgc gatgaaagcc acggcagaag ggccgaagcg gatggattgt
     2281 gcatctggat ttggcgcggc aacgatggtg accgccacct ttgggtttgt tgcggtttct
     2341 catgcgctga agaagatgat ggcgaaagcg gcgcgtcagg gttaaacaat caggtcggat
     2401 gcgacgcgaa cgtcttatcc gacctggttc tgtgcttaaa cctgcttcgc cgcagcgata
     2461 atcgcctcgc ttaacgcatt caacccctgg ctgcgtgagg cgctaagctg tgcacgcaat
     2521 cccagctcat caaacaatgc cagcggcgac tgtgcctgca attcggcgac ggttttcccc
     2581 tcaacggcag tcaacaacac cgccaacagg ccgcgcacaa tgcgcccttc gctgtcgcca
     2641 aagaagtgca ttttaccgtt ttcagtcacg gtatatccca gccagacgcg gttttcgcat
     2701 ccggcaatct ctttagcctg cgcttttaac tcgtctggca aagccggaag ctgtttcccc
     2761 agcatgatca actggcgata tttatcttcc cattgcgaca acggtgcgaa ggtattgcgt
     2821 aacgtttctg cggttacggt tgtgccgaac ggatgtccgg cgaattgcgg gtttgtcatt
     2881 aatccaccaa taattccagc gcgcggtcaa cggcattcac cagcgcatcc acatcactct
     2941 ttgtattata tggcgcaaaa gaggcgcgca gtgtgccggt tacgcctaat tctgccagta
     3001 gcggctgagc gcaatgctgc ccggcccgca gggcaatacc gtactccgcc agcagcgtca
     3061 ccatatcgct atgatgaacg ccagcaaaat caaaggccag caggctggaa tcctggcagc
     3121 ggaatgaacg aaagccggga cgtttcgcca gcgcatcttc cgccagcgtt gctaagctac
     3181 ggctccagct ttcggcctgg ttgatatcgt aatctgccag ccattccagc gccgcgctta
     3241 atcctatgac accagcgaca tttggcgttc cagcttccag tttccacggc gcagattgag
     3301 tcgtgaagcc gtcaaaactc acttcgtgaa tcattttgcc gccacccagc cagggcgaca
     3361 tcgcttccag cagttctgat ttgccataca gcacgccgat acccgtcggg ccatacagtt
     3421 tgtgaccaga gaaagcatag aaatcgatat ctagttgctg aacatctgcg gggaaatgca
     3481 ccgccccctg agcaccatca accatcacca ccatcccggc tgaatgagca aaggtaatcg
     3541 ctcgcgccag atccgggcaa ccgccagtaa cgttcgacat ctgacccaac gccagaatcc
     3601 gactacgggg agtaatcagt tctggcaaca aatcgacatc cggcagtcgc tgcgcattaa
     3661 gcggcaattt caccactttg gctccagttt gttgggcgac catcagccag gggacgaggt
     3721 tagcgtggtg ttctgccacg ctgacaataa tctcatcgcc cggttgcaga cgcggacgcg
     3781 catagcattg tgccaccatg ttgatggatt cagtggtgcc gcgcgtccag acgatagttt
     3841 tatcatccgg tgcattcagt aattgcgcca ctttctctcg tgcagcttca taacgcgcgg
     3901 tcaggcgttg ggcttcggca aactggctgc gatggacgtt tccggcgctc aggctgtaaa
     3961 actgtcgggt ggcttcaacc acggcttcag gtttaagcgc ggtcgcggcg ctgtcgagat
     4021 agacgcccgc atcctgtaga gcgggaaact gggcgcgaaa ctgcgcggga ttaaaaacgt
     4081 tcatggtact cctcggcttg atgcaccgat cgtgacgcaa aattcacttt gatacaagga
     4141 gagtgctaac catcagaatt atctggatat cctggcgaag ccgcgtaagt aacatatgct
     4201 gaatagtgtt aggcgttaat tttagcctgt taatcaatac tattaagatt tattaattgc
     4261 tttaaggaga agatgatgaa aaagactgcc gcaattattt ctgcctgtat gctgactttt
     4321 gccctgagcg cctgttccgg ttcgaactat gtgatgcaca ccaatgacgg acgtaccatc
     4381 gtctctgacg gcaaaccaca gactgataac gataccggta tgatttcgta taaagacgct
     4441 aatggcaaca aacagcagat caaccgtact gacgtgaaag agatggtcga actggatcag
     4501 taattcacga tcgcaaggta aaaaaaagca ccgcaattag gcggtgctac attaatcact
     4561 atggacagac agggtaaatg tacaggaagt gaaaaaaggt agctttgcta ccatggtctg
     4621 aatcgcagac caattgcaaa cacaacaaca caacatcaca accgtaagcc aaaagttcac
     4681 cagaacacgc attccgataa aacttttcgt tccggctcag gaagtgccgc cactataggt
     4741 atttgctggt agaagctcaa cggacaattt ataatggctc agattaaaaa aactaatagg
     4801 ttacacagtg tgatctaatt gttaaattca tttaacatca aagtttaaaa gccatgtcta
     4861 aacgattacc accgctaaat gccttacgag tttttgatgc cgcagcacgc catttaagtt
     4921 tcactcgcgc agcagaagag ctttttgtga cccaggccgc agtaagtcat caaatcaagt
     4981 ctcttgagga ttttttgggg ctaaaactgt tccgccgccg taatcgttca ctcctgctga
     5041 ccgaggaagg gcaaagctat ttcctcgata tcaaagagat attttcgcaa ttaaccgaag
     5101 cgacgcgtaa actccaggcc cgtagcgcca agggggcgtt gacggtcagt ttactcccca
     5161 gtttcgccat tcattggttg gttccgcgac tttccagctt taattcagct tatccgggaa
     5221 ttgacgttcg aatccaggcg gttgatcgtc aggaagataa gctggcggat gatgttgatg
     5281 tggcgatatt ttatggtcgg ggcaactggc cggggctacg ggtggaaaaa ctgtacgccg
     5341 aatatttatt gccggtgtgt tcgccgctac tgctgacagg cgaaaaaccc ttgaagaccc
     5401 cggaagatct ggctaaacat acgttattac atgatgcgtc acgccgtgac tggcagacat
     5461 atacccgaca gttggggtta aatcatatca acgttcagca agggccaatt tttagtcata
     5521 gcgccatggt gctgcaagcg gctattcacg ggcagggagt ggcgctggca aataacgtga
     5581 tggcgcaatc tgaaattgaa gccggacgtc ttgtttgccc gtttaatgat gttctggtca
     5641 gtaaaaacgc tttttatctg gtttgtcatg acagccaggc agaactgggt aaaatagccg
     5701 cctttcgcca atggatcctg gcgaaagccg ctgctgaaca agaaaaattc cgctttcgtt
     5761 atgaacaata atttacgtag ggtacgacca tgaccagccg ttttatgctg attttcgccg
     5821 ccattagcgg cttcattttt gtggctctgg gcgcttttgg cgcgcatgtg ttaagtaaaa
     5881 ccatgggggc cgttgagatg ggctggatcc agaccggcct cgaataccag gcgtttcata
     5941 cgctggcgat cttaggtctg gcggtggcaa tgcagcgtcg catcagtatc tggttttact
     6001 ggagtagcgt tttcctcgcg ttaggcacgg tgctgttcag cggcagcctt tattgcctgg
     6061 cactgtccca tctgcgtttg tgggcgtttg tcactccggt tggcggcgtg agcttccttg
     6121 cgggctgggc gttaatgtta gttggtgcta tccgtttaaa gcgcaagggc gtaagtcatg
     6181 aataaggttg tattgctgtg ccgtccgggc tttgaaaaag agtgcgccgc agaaattacc
     6241 gataaagccg gccagcggga aattttcggt tttgcccgcg tgaaagagaa tgcgggttat
     6301 gtcatttatg aatgttatca acctgatgat ggcgataagt taatccgtga gctgccgttc
     6361 agttcattaa tttttgcccg ccagtggttt gtggtggggg aactcctgca gcatttgccg
     6421 ccagaagatc gtattacccc cattgtcggc atgttacagg gcgtagtaga gaagggcggt
     6481 gaactgcgtg ttgaagttgc cgataccaac gaaagcaaag agttgctgaa attctgccgt
     6541 aaattcaccg tgccgctacg cgctgccttg cgcgatgccg gggtgctggc gaactatgaa
     6601 acgccgaagc gtccggttgt gcatgtattc ttcattgcac caggttgctg ctataccggt
     6661 tactcataca gcaacaataa ttcgccgttc tatatgggca ttccgcgcct gaaatttccg
     6721 gcagatgcgc cgagtcgttc cacactcaaa ctggaagagg catttcatgt gtttattcct
     6781 gcggatgagt gggatgaacg cctggcgaac gggatgtggg cggtggattt gggcgcttgc
     6841 ccaggcggct ggacctacca actggtgaag cgcaacatgt gggtttattc cgtcgacaac
     6901 ggcccgatgg cgcaaagtct gatggatacc gggcaggtga cgtggctgcg ggaagacggt
     6961 ttcaaattcc gtccgacacg cagcaatatc tcctggatgg tatgcgatat ggttgaaaaa
     7021 ccggctaaag ttgcggcatt gatggcgcag tggctggtta atggctggtg ccgtgaaacc
     7081 attttcaatc tcaaactgcc gatgaaaaaa cgctacgaag aagtatcaca caatctggcg
     7141 tatattcagg cacagcttga tgaacatggc ataaatgctc agattcaggc gcggcagttg
     7201 tatcacgatc gcgaagaagt gacggtgcac gtccgccgca tctgggctgc ggtgggtggt
     7261 cgtcgcgacg agcgataaca ggcaaggccg gggcgtcgtc tccggcctgc taccctttcc
     7321 gtcaggctgt taccaaagaa gttgcaacct ggcgctcatc agaaataatg tgcgttacct
     7381 catccagatg cccgatgcgg gcttcccctg aacgattaaa tttactttta tcaatcaata
     7441 acaacgattg cgcggcacgt tttaatagca tcgatttgta atcagcgttg atggcattgg
     7501 agtcccacag tgcgccgctg ctatcgatcc cttcacaaga aaaaataaac agatcgattt
     7561 ccagcgattt aagttgggaa atcagcgagg gattgacgta acagccatat ttgcgctcaa
     7621 gtgtgccgcc ggaactgatc agttgaatgc gttcgcgttt accgagttca tggcaaatcg
     7681 gatggctatt ggtgaagacc tgaatgttga tgtcaggcaa ctggcgtgcc agataccagc
     7741 aagttgaact ggcgtctaag gctatcacca tcccttcttc aatccacgcg agcgcctcgc
     7801 gcgcgatatc tgctttatgc gcataatggc ttttcagccg gatgtgaaag ggatcgccac
     7861 tgtcttgatt ttgacggtgg atatatttag cgcgtccatg attgcgcaga attttaccct
     7921 gcgtctgtaa ttcattgaga tcgcgacgaa tggtttcttt actgaccttt agctgttcag
     7981 agagagcttc cgtggtcagg ctggtatggt tcagcagcag gtcgactatc gcttgctggc
     8041 gtgccgcttt catatttttc accccatcat tcgataaccg cccgcgtcat gcgggcgtac
     8101 cggtatgaga ttacggtgtt accccttttt ttaaaagaat attcccgtac ttcgctcgtt
     8161 cgcctgtgat aacgatcgca aaggcttttt gcgcccgttc ataaaacgca aaacgattga
     8221 tgcggatgat gtcaggacac ggggcttgta gtgaaagcgc attacggtaa cgtcgttcta
     8281 cttcaggatc gagagtgtca ccttctaccg ctgccatcat caccagcggc ggggcataac
     8341 tgtccagctc aaataacggg ataatcgcct ggagcaagtc gctcaccaac aggccatcag
     8401 cgcggatcac ctgcggtccc atcgaatggg cgggaaagtg agcatcggaa aaaataattt
     8461 catctccatg tcccatctct gccagcactt tcaatagttc gggagaaatt aacggcgaaa
     8521 ttgttttcag catttcacac ttcctctata aattcaggtt cagtttgcgg gtagaaataa
     8581 cggtactgat aatgaatctg tgcacgggct tcttccgggc tgttgaattc ccctacgcca
     8641 taccaaccga acagcgcagc tcctgcgacg gtcgtttcgg catcgtcgag aacttttacc
     8701 ggaatatcaa gcatattggc tttaatctga ttccacaatg tgttgcgact tcctccaccg
     8761 actaacaata attcagaggc cttaaagtgc ccgatttttt ccagcatctg tagattgcgc
     8821 tgtaattgcg cagttaaccc ttccagcgcc gcgcgataga aatgcccccg cgtggtatta
     8881 agcgtcactc cttgccagcc agcgttctga cacgacaata aatcacactg catttttacg
     8941 ccatccgcgc caggcgcgat cagacgagct tcttcaatca acatttgcca gggtgtttca
     9001 gccgtccaga acagttttct cacccattcc agcacgccgg atgccagcca ttgcatacct
     9061 gggttataca accctgcctg gctatccagt tcgcaggtgg aaccggcgta ctgacttaac
     9121 agcgaagtat caacctgggc gctacggacc attaaaattt cccatgtacc ggaagagagc
     9181 acgggttcat tttggtcggc accagcgcca aaaagggcga actgggtatc gtgacctgcg
     9241 gaaatcaccg gtatgccaac gggtaagccg agcattgctg cggcgctgtt ctgtagcgta
     9301 ccaatctgtt cacccgcttc caccagacga gggaagagtc ggcgtggaat accggtggct
     9361 tgtaaaattt gcggactgaa atcgcgttgc tggatatcca gcatctggct ggttccggcc
     9421 atcgtgatat cagtagtgaa ttcgccggtt aaacggtggt taatcagcga cgaaataaag
     9481 agccaggcgt gcgcgcgttc cagcagttgt ggatgatttt ctttcaacca caccaactta
     9541 tataacgtat tgaaactaaa ggctccgacg ccagaaatag cctgcaaccg ctgtgcggag
     9601 atcaactgtt caatattgtc cataaccgct gctgttcgcg gacatttcca gctaatgatc
     9661 ggatagagca gattgccttg cttatctacc agagcgccat ccacaccaaa ggtggtgacg
     9721 gcgataccgc ggatgtggca ttcagtcagt tcactattga tttgccgaca gcaatcagca
     9781 aagcgttgca aaatggcgtc taaagaccac tggtgccagg tgttgttttc catcgcgata
     9841 tcgctggcat taggcgttga ggcgcgggca acaatttttc cctgccgatt aaccgcgatg
     9901 gccctgacat tggtcgcgcc acagtcgagt accaggataa cttcttgttt cataatcgct
     9961 cctgcaatat agccggataa cattgcttat ccggctaacc actcttgctt gttcaggccc
    10021 ggtagccgga gcgaccgggc atcacatcag ggagtaatgt attaacgctt gtacaacggg
    10081 ccgtagttct ggcaagcgcg gtaatcctgg ccttcaatat ccatgccgtg cgcagcccag
    10141 gcagaaggac gatacacttt ggtctcttca acgttgtgca tacataccgg gatacgcagc
    10201 atggaggcga gagtgataaa gtctgcgcca acgtggccga tggtcagaac cccatggtta
    10261 gcgccccagt tcgccattac cgagtacaca tccgtaaacg gccctttacc ggtgaggcgc
    10321 ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt tgttgaggat gtcatgcaca
    10381 tccttcggca attccacgct ccagccttcc gcgatttgca gtaccggtcc caggcctttg
    10441 atgatgttga cacgggtcat ggtgaacggg acgccgcctt cggtaaggaa gcgggaagag
    10501 taaccgccgc cacggaagta ttcgtggatc gccgggcacc attcggtagc ggcgaggcaa
    10561 gcgtcagcct cttgctgaga gatttcccag tgcggcttca tcgtcgggtt accttcgctg
    10621 tcgcgttgtt tacaggaacc gtccagcgca gcagaaccgg agttgatcaa atggatgatg
    10681 ccgtgttctg ccagtccatc gagtttatgc cccgttacac gctcaattgc ttctggtgac
    10741 cagtaggtac gcacatcggc aaatacctga gcggtgccgg tgagctggtg acccattagc
    10801 attgccacgc cgttaagact gtcgttttcg gtcgccacga caaagggttc gcgcacgcca
    10861 ttccagtcaa atgaactgtt aaggatcgct tcggcggtgt caccattggg atattgatcg
    10921 gtccagtgac gttgcccctg gaagcccgca gcgatggcgt tgtagccaag tgattcttcc
    10981 acgcgaccaa tatcggccag tttgctgttg ccttgcatca tgtcgcggat acacatcgcc
    11041 atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg cattgcgttg atactgtttg
    11101 ttattttcat cttcgccata gcggaagttt ttatcagccc aggtcagtgc catttccagt
    11161 tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca gttcggtcat atccaccgcc
    11221 tggactttca ttcccagcca ggattcaaag aagttgtgat caacaataga accggcgata
    11281 cccatcgaaa cgccgcccag cgacagatag cttttacctt tcatgctggc gacggccaaa
    11341 ccggcgcggg caaagcgcag cagtttttct tcaacatcgg caggaatcga tgtgtcatcg
    11401 gcatcctgaa cgtcatgacc gtaaatggag aatgccggga tgcctttctg gctgtgagct
    11461 gccagagccg ctgccaggta aacagcgccg ggacgttcag tgccgttaaa gccccaaatg
    11521 gctttcgggc gggttggatc catgtcgatg gtttcactgc catagcacca gcaaggcgtt
    11581 accgtaatgg tgaggcctac attctgactg ctgaattttt cttcgcaagc agcggcttca
    11641 gccatacccg cgatacaggt atcggaaatg acacactcga cggcagctcc gcaggcatgg
    11701 cgcagtttct cggtcagcag tgcggccgta gctttcgcca tattcattgt ttgttcttca
    11761 agcgactcac gaacacccat gcgacgaccg tcaataaccg ggcggatacc aattttcggt
    11821 aagctgattt ttttcattac agattcctca ctttattcgg aaaatatgtt cagttagttg
    11881 ccgtttgaga gcggaaacgg gcaaagataa agatgaccgc gaagcagagt gcggggatca
    11941 gttcagcagt ggggatgttg cccgccgcgt cactgacaaa acccatgacc ggagtgacaa
    12001 taccgccgcc aatgatggtc ataacgatga aggacgaacc gtatttggtg tcctggccga
    12061 gattcttaat gcccagcgag aagattgttg ggtactgaat cgacataaag gcgctgcata
    12121 aagtcagggc tattaagccc acatgaccgc cagcgaaggc tgagatcagg cacagtgcca
    12181 tagcgattaa tgcgtaggcg gccaggactt tgtgtggtgc gaagcgactg atgagccagg
    12241 taccggtgaa acgaccaata aagaagcaca ccatggttcc ggttaaatag ttagcggcaa
    12301 agcctgcagt catacctgga atttcttcta cagcgtagcg aatcaaatag ctccagcagg
    12361 ccgtttgtgc gccaacgtag cagaattgcg ctaataccgc ccagcgccag tgacgaatac
    12421 gcgccagacg agaaagcgat gcggagaacg atccttgttt ggcgtcactg tgattatcac
    12481 tctgcaatgc cgggaatttc gtcagcatga tcagcagggc gaccagtaac acgatagcca
    12541 cgatgatcat ataaggtgtc tgtaccgata ataccaggct gtgtttatac gcactcaatt
    12601 gctctggaga cattttatcg agaacgtctt gcgattgatg tggcacgtta gacaaaataa
    12661 gactttgccc aaagacaacc gcgataattg cgccaaacga gttaaatgtt tgcgcaagat
    12721 ttaagcggaa gtgaccactg ctttccggcc ctaataccgt aacaaaaggg tttgcggcag
    12781 tttccagaca acctaatccg gctgcaataa taaataggcc aactaaaaat aaggtgtagt
    12841 tcattatttc tgcagcgggc cagaataatg cagcgcccaa ggcatataaa aataatccgg
    12901 taataatccc tgctttataa ctgagttttt tcatcaatat cccagcaggg attgggataa
    12961 tgaaataacc aaagtaaaag gccgattgga tcaggccagc ctggaaattt gtcagcgtaa
    13021 aagcctgctg gaattgaggt aataaaatgt cgttaaggtt attggctacc gcccaaagaa
    13081 aaaacagtga gcacagcagc gcgaatggaa taatgtaact tctgctttgc cctgcatctt
    13141 tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt tcccatagca tcctcttagg
    13201 ttcagaagct taagcacgta atcggttatg ttttcaaaag tgcaaaacca gcgagttcgg
    13261 gcttcatatc agtgaatatt tgcctgtctg aaatgaactc atggattcat tttcaaataa
    13321 aaaacgctct gtaatatgac ggcggtcaca cttattagct ggaaggtgac tttatgtgac
    13381 taccatcact ttaataatcg aaatttcatg cccgtttcag aaaataaatt ataaaaaaca
    13441 caaaccagat gaccgtatta attgtttatg ccgaaaacgg gcattcctgg cttgagtgaa
    13501 tagaaaaatg accgttttga taaatatttt taagagcggt catttttaat ggggttataa
    13561 aaagagaact tgcagttatt tactgtgatt agttgaacca ggtcacaaaa tataagcttg
    13621 tgaatttctt tctggttaat caggaagaag atggtgggta attaaacggc taattcaata
    13681 gtgtgaaagg aacaacatta ttgccctgtt ttgaatcaga gagaggtagc taatggaacg
    13741 aaataaactt gctcgtcaga ttattgacac ttgcctggaa atgacccgcc tgggactgaa
    13801 ccaggggaca gcggggaacg tcagtgtacg ttatcaggat gggatgctga ttacgcctac
    13861 aggcattcca tatgaaaaac tgacggagtc gcatattgtc tttattgatg gcaacggtaa
    13921 acatgaggaa ggaaagctcc cctcaagcga atggcgtttc catatggcgg cctatcaaag
    13981 cagaccggat gccaacgcgg ttgttcacaa tcatgccgtt cattgcacgg cagtttccat
    14041 tcttaaccga tcgatccccg ctattcacta catgattgcg gcggctggcg gtaattctat
    14101 tccttgcgcg ccttatgcga cctttggaac acgtgaactt tctgaacatg ttgcgcaggc
    14161 tctcaaaaat cgtaaggcaa ctttgttaca acatcatggg cttatcgctt gtgaggtgaa
    14221 tctggaaaaa gcgttatggc tggcgcatga agttgaagtg ctggcgcaac tttacctgac
    14281 gaccctggcg attacggacc cggtgccagt gctgagcgat gaagagattg ccgtagtgct
    14341 ggagaaattc aaaacctatg ggttacgaat tgaagagtaa tttcgtaaag caacaaggag
    14401 aaggatgatg gctaacagaa tgattctgaa cgaaacggca tggtttggtc ggggtgctgt
    14461 tggggcttta accgatgagg tgaaacgccg tggttatcag aaggcgctga tcgtcaccga
    14521 taaaacgctg gtgcaatgcg gcgtggtggc gaaagtgacc gataagatgg atgctgcagg
    14581 gctggcatgg gcgatttacg acggcgtagt gcccaaccca acaattactg tcgtcaaaga
    14641 agggctcggt gtattccaga atagcggcgc ggattacctg atcgctattg gtggtggttc
    14701 tccacaggat acttgtaaag cgattggcat tatcagcaac aacccggagt ttgccgatgt
    14761 gcgtagcctg gaaggacttt ccccgaccaa taaacccagt gtaccgattc tggcaatccc
    14821 caccacagca ggcactgcgg cagaagtgac cattaactac gtgatcactg acgaagaaaa
    14881 acggcgcaag tttgtttgcg ttgatccgca tgatatcccg caggtggcgt ttattgacgc
    14941 tgacatgatg gatggtatgc ctccagcgct gaaagctgcg acgggtgtcg atgcgctcac
    15001 tcatgctatt gaggggtata ttacccgtgg cgcgtgggcg ctaaccgatg cactgcacat
    15061 taaagcgatt gaaatcattg ctggggcgct gcgaggatcg gttgctggtg ataaggatgc
    15121 cggagaagaa atggcgctcg ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt
    15181 agggttggtg catggtatgg cgcatccact gggcgcgttt tataacactc cacacggtgt
    15241 tgcaaacgcc atcctgctac cgcatgtcat gcgctataac gctgacttta ccggtgagaa
    15301 gtaccgcgat atcgcgcgcg ttatgggcgt gaaagtggaa ggtatgagcc tggaagaggc
    15361 gcgtaatgcc gctgttgaag cggtgtttgc tctcaaccgt gatgtcggta ttccgccaca
    15421 tttgcgtgat gttggggtac gcaaggaaga cattccggca ctggcgcagg cggcactgga
    15481 tgatgtttgt accggtggca acccgcgtga agcaacgctt gaggatattg tagagcttta
    15541 ccataccgcc tggtaaatgc gctgatgtga taatgccgga tacgacgttt gcggcgtatc
    15601 cggctcgccg ttaccgtacc aaccgcaatt gctgtaaatt gccgtcgata tgcaaatcgg
    15661 tttgtaagcg ggcaatatcg cggcacagaa acgccatctc tttatgggtt tctaattttt
    15721 tgcgccactt ttcggcaacc gcatccagat tctcatatat cccttccaga ctctgaaact
    15781 cgaccagcag ctgcgtggcg ctttttggtc cgattcccgc aacacccggt acctttgaac
    15841 tgctgatccc cgcaagtccc cagtaatcgg gcaactgctg cggttgaacg ccaaattctt
    15901 tatcgataaa tggcgcatcc agccaacgtt tctggaagta atcacgaata cgtaatgtcg
    15961 gtgaaagtaa ctgacagtag cctttatctg tcgaaacaat cgttgcctga tgcccggcct
    16021 gtgtcacttt gaccgccagc gtggcggcta agtcatcggc ttcgttgccg ctggttgacc
    16081 agcacgggac gccgcgttgc tcaaaggcgg cgcgtaatgc aggcatctcg tcgtgcaact
    16141 cttccggcat tggcggtcga cccgctttgt aatctggtaa acgctgatga cgccagccgc
    16201 tactgcggtt ttcatcatca aaaacggcga ccgcgtgggt tggctggctg tgcataatga
    16261 gctgatcgag cgcatgctga caggtttcga cacagggcga tccctgaacg gcatgaatgc
    16321 gacgaataag attcagtgca tcgacaataa gcaaatgaac agccacgata acccccttaa
    16381 agatcaggcg ataagggtaa cattcgatgc cggaacaggc tacggctgga tgagaaatcg
    16441 ggaagaggcc tcgcaaaacg aggcctctgg agagcgatta atcgcaggca acgatcttca
    16501 ttgccaggcc gccgcgagag gtttcgcggt acttggcgtt catatcttta cctgtttcgt
    16561 acatggtttc gataacttta tcgaggcaga cgcgcggctc gctggtacgg cgtagcgcca
    16621 tacgtgcggc gttcaccgct tttactgccg caatggcgtt acgctcgatg catggcacct
    16681 ggacctgtcc ggcgaccggg tcacacgtca gaccgaggtt gtgctccatg gcgatttccg
    16741 ccgcgatgca cacctgcgcc gggcttgcac ctaacagttc tgccagacca gccgccgcca
    16801 ttgagcacgc cacaccaact tcaccctggc agccgacttc agcaccagaa attgacgcgt
    16861 tcatcttata aagagaacca atggcactgg ctaccagcag gtaacgagcc agtgagttag
    16921 cgttcacttc gcggataaac ttgtcgtagt acgccagcac tgccgggata atcccgcacg
    16981 caccgttagt cggcgcagtc accacgcgtc cgccagcagc gttctcttcg ttcactgcca
    17041 gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc agtggtggtt ttatcctggc
    17101 tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac gcgcagtttg ccaggcaaca
    17161 cgccttcggt ggaaataccg cgctcaatac cgccacgcat gacttcccat acgttcgcca
    17221 ggtgctgttc cagctcttct ttgctgtgca gcgccagctc atttttcatc atcaggccag
    17281 agagtgacag cccggtttcc tgacaatgtt tttgcagatc ggctgctgaa ctgtacggat
    17341 aaggaacttc aaccggtgcg ctattctgct ggccaaaatg ctcttcatca acgataaagc
    17401 caccgccaat agagtagtaa gtctggctgt aaacgacttt atcgcccgcc agcgcggtaa
    17461 tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg gaagttcatg cactgatcaa
    17521 ccgggaactc cacttcatgc tgaccgtttg ccagcatcag gcgaccatga gtattcacat
    17581 cctgaataaa accagggatg gaatcgatat ccacggtatc cggcaggtta cccgccaggc
    17641 ccataataat ggcgatatca gtgtggtggc ctttaccggt cagagagagc gagccgtaca
    17701 cgtcaaccac cacgcgggtc acgtctttaa gcagattacg ggcaatcaga tcgtcggtaa
    17761 attgtttacc cgctttcatt ggtccaacgg tatgagaact ggaagggcca atgccgattt
    17821 tgaaaatatc gaatacgcta atcataggaa atacatcgcg ttaaaacgga ggaagcgccg
    17881 cccgaaagcg gcgcgaaagg acttagctga acagagagta gaagattgcg gagattgcaa
    17941 tcagacccat cacgacaacg aatacgttgc tgatgtgacc gctgtactta cgcattgccg
    18001 gtactttctg aattgcgtac atcggcatca ggaacaggat catcgcgatg attgggccgc
    18061 ccagggtttc aatcataccc aggatgctcg ggttcagggt ggcaacaatc caggtcgtta
    18121 ccagcatgaa cagcgcagtg atacggttca gcttgttgat ttcgatagac ttacctttac
    18181 cacgcagaga tttaatcacc ataccgttga agccttcacg tgcgcccagg tagtggccga
    18241 ggaaggattt ggtgatagcg ataatcgcga taatcggagc catccacgcg ataaccggtg
    18301 cgttaaagtg gttagccagg taagacagaa tcgagatgtt ctgctcttta gccgcagcca
    18361 ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa gaacattacg gtcagcacca
    18421 tcatgatgtg tgcgaatgca aggatcttcg agcatttctg ttctgccata tcgccgtact
    18481 cttcacgctt cgcaacggcg aaagaagaga tgatcggaga gtggttgaac gagaacacca
    18541 ttaccggaat tgccagccac agggtcatcc acagaccgtt cccggttgca gatgcagtgt
    18601 ccagagacag cgtttccagt gctgcgccgt tccactgcgg gatcaggtac agagccagca
    18661 gcatcagtac gccaacaaac gggaatacca gaatactcat cgctttaacg atcatctgct
    18721 caccaaagcg aacgatggtc atcataccca cgatcaggat cagcgacaga atcgcacgcg
    18781 gcggtggcgt catacccagc tggtgagaca tgaagctttc aactgtattg gtgattgcca
    18841 cgctataaac cagcaggatc gggtagatag cgaagaagta gagcagggta atcagtttac
    18901 ctgcgccaat accaaagtgt tcttctacaa cctcggtgat gtcttcgccc gggtttttac
    18961 cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa cgtcatcggg aacgcaagga
    19021 tagccatgat gatcagcggg atcataccgc caacaccggc gttgattggc aggaacagca
    19081 cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca catggtgtct gtcttgcgcc
    19141 aggcactacg agagtctttc gacgcaatcg tgctggtttg agtcgtttcc atctatttct
    19201 cctggaggaa tgcaaataat tcagctttta gccagttcag cccgggaagg tgcctgaccg
    19261 taaaaatgaa atctgtctat cggcgatggc ttaatcattt accgccgtaa taatttgagg
    19321 gcggaaagat acacacaagt tatgtatcta tcagtgatct tgatctcaaa tacaataatc
    19381 tacttggaag atagattatc ccaacgcttt actctgtaaa tcaaaagtaa aagagaattt
    19441 aagggcaata tttgcccatt aaacgacatc tgacttcttc cggggcgcgc aatatatgta
    19501 ggttgaactg aaaaaatcaa tacttttagt taatcatggc gataatttct ttttcatagc
    19561 gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt gcaacataaa ttgtatgaca
    19621 tattaactgt gatgatattt gtcgcgtgag ggtggtaaga aacattcata taacaaatga
    19681 aatgagttga tgagtaagtg accaaaaacg agaaaacccg gcaacaaacg tcaccggggg
    19741 agcggagatt acgtgcagat ttcgtagcaa gggatgtagg ctgagcctgg caacttcata
    19801 cgatgctggg caacaaaacc ctgtagcagg tcgtccatac gacgcataat ctctttatcg
    19861 ccgttgattt tgtaaggacc aaactcttca atggcgcgaa taccgacttc ttttacgtta
    19921 cccgccacaa taccggagaa cgcacggcgc aggtcggcag ccagcacttc aacaggttga
    19981 tccgggtaaa gcttcagatt agccatattc tcgtgagacg gctcaaacgg catttgcaaa
    20041 tctggtgcaa tgcgcattga ccagttaaag ctgtaagcgt caccagtgtc gcggcgattt
    20101 tctttcacca gcggcatcga ttttttcatc tgacgagcga cttcagcggc gtcatcaatg
    20161 atgatgcggt aatggcggcg cgcgttttcg cccagcgtat gtacgacaaa ctcgtccagt
    20221 acgcggaagt agtcggcgct ctctttcggg ccggtgagga tcaatggtaa aacctgatct
    20281 ttgttggccg ggttcattaa aattcccagc aaatacagca actcttctgc cgtacccaca
    20341 ccgccaggga agataatgat gccgtgagcg atacggacaa acgcttccag acgtttttcg
    20401 atatccggca tgatgatcaa ttcgttgacc agcgggttag gcggttcagc ggcgataatc
    20461 gacggctctg tcataccaat aaaacgactg tctttgtaac gctgctgcgc gtgtccgacc
    20521 gcagcacctt tcatcggcgc ttccatcgct cccggaccac agccggtgca gatattcagc
    20581 tcacgcaggc ccagctggtt tccgacgcga cgggcataca aatactcatt ttcgttaatt
    20641 gagtgaccgc cccagcagac caccatattt ggcgcttcac ccacatgcag cgcgcgagcg
    20701 ttacgcaaga tggaaaagac caggttagtg atgtggacgg agttgtcgag attcaggttg
    20761 ggaaaacgaa cggtgttatg gatttgcccg taaacgaaga gaatgtcacg caggaccgcg
    20821 aacaagttgg cctgcaaagc gcgaataatt cgcccatcga caaaagcctc ttccggggga
    20881 ttaatcagtt ccagctttac gccgcgttca cggcgcaaga cgttaatatc gaaattttca
    20941 aaacgagaca gcaattcttt gctgttatcg gtcaaactac cggagttcag tacggcaagt
    21001 gaacagttgc gaaacagttg atagaggtcg ctgctggcgg tgcgtttaag catatccact
    21061 tccagctgcg acaacatatc catggagcca agcgggctaa tatgtgtaat caagaaaaac
    21121 tccttatggg acgaatatcg tcattccctt tgattacaat agccctgcct gatgaccttt
    21181 cacaaccttc tgcgtgaatc cagcacgcaa aattgagaaa aaatttattg ccgaaccagt
    21241 cttgtggtcg atgggacaaa atcgctatta ctgcgccacg ggttaatgtc cagaccgcca
    21301 cgacgggtat aacgtgcgta aacgctcaat ttttctggct ggcagaagcg taacaggtca
    21361 ttaaagatgc gttccacgca ctgttcgtgg aactcgttgt gatgacggaa tgagaccagg
    21421 taacgcagca gtttttctct gtcaatttgg cgtccacgat actgaatttg gatcgaaccc
    21481 caatctggtt gatgggtgat caggcagttt gatttcagca ggtggctgac aagcgtctct
    21541 tccactactt tttcaccgct ggtggcattc tccagatagt cagtagtgaa ttcatagtta
    21601 tcgatagtga tatcctggtc atcaatgcag gtgccattga aatgacctat cggctggcct
    21661 tccagttcat caagacgata taacgccacg ctaaccttac cctgggcgca agtgcttaag
    21721 tcgcgctcca gcgtctggcg cacctcatcc cagttattaa aacgcgtctg gttaaaactg
    21781 ttgagataga gtttaaaact cttcgactca atcagattta cgctggtgta atcaagttca
    21841 acatgaccga cagcgacctg cggcaaacct ttcgcattca gccaggaaag ttcatacagc
    21901 gtccagatat ccgtaccgtg aaaaggcagg ttatccgctt tcagacccag cgggtcgcga
    21961 ttcaggctgc gtggaacgcc ttgcagtagg ctggcgtcat aggtatcccg gtaatcggtt
    22021 gattttccaa gagtcaggcc cgcaagtgcc tgatggtttg cataagaaga catgtttcac
    22081 cgtcataaac cagataaact aaacgacaag tgtatctggt ttaagtgaag agagaaatta
    22141 gtggacgatt tgaccgcaca agccctgaaa gattttactg cacgctactg cgatgcgtgg
    22201 catgaagagc ataaaagctg gccgttaagt gaggaactgt acggtgttcc ttcaccgtgc
    22261 attatttcaa ctaccgaaga tgccgtatac tggcaacccc agccgtttac cggggaacaa
    22321 aatgtaaacg cggttgaacg cgcttttgat attgtgatac aacccacaat tcataccttt
    22381 tatacgactc agtttgccgg ggatatgcac gcgcagtttg gcgatatcaa actgacattg
    22441 ctgcaaacct ggagtgaaga cgacttccgt cgggtgcagg aaaatctgat tggtcatctg
    22501 gtgactcaga aaaggctaaa actgccgccc acgctattta tcgcgacgct ggaagaagag
    22561 ttagaggtca tctcggtgtg taatctctcc ggtgaagtgt gcaaagagac gctgggaaca
    22621 cgtaaacgga cgcatttagc ctcaaatctt gcggaattcc ttaatcaact taagcctctt
    22681 ctgtaatcca aataccccat ctggttgtga gagatctctt acagactctg taggagatcg
    22741 ccaggaaata agcgaatact taaaaagata agaatcgtta ttttcattta aaatcaaaat
    22801 gttgattgtt aattcttaac tttcatatga aattttcctt aaggcattgt ctgtaagcgc
    22861 cttgtaagac ttcgcgaaaa agacgattct atcttcgtcg acagggagtc agacaacgaa
    22921 gtgaacatca ggatgatgac acttctgcag gacacaccag gatggtgttt cagggaaagg
    22981 cttctggatg aagcgaagag gatgacgcag gacgcgttaa aggacacctc caggatggag
    23041 aatgagaacc ggtcaggatg attcggtggg tcaggaaggc cagggacact tcaggatgaa
    23101 gtatcacatc ggggtggtgt gagcaggaag caatagttca ggatgaacga ttggccgcaa
    23161 ggccagagga aaagttgtca aggatgagca gggagcaaca aaagtagctg gaatgctgcg
    23221 aaacgaaccg ggagcgctgt gaatacagtg ctcccttttt ttattcctgc tatccttcgc
    23281 ggcagttttt cttattgagg ttgctttatg accactcatg accgcgttcg tctccagttg
    23341 caggcgcttg aagcgttact gcgtgaacat cagcactggc gaaacgatga accccagccg
    23401 catcaattta atagtaccca accgttcttt atggacacca tggaaccact ggagtggttg
    23461 cagtgggtgc tgatcccgcg aatgcacgat ttgttagata acaaacagcc gttaccgggc
    23521 gcttttgccg ttgcacccta ttatgaaatg gcgctggcga cggaccatcc gcaacgcgcg
    23581 cttattctgg ctgagctgga aaaactggat gctctttttg cggatgatgc gagctaatgc
    23641 tggaaatact ctatcaggat gaatggctgg ttgcggtaaa taaaccctcc ggctggctgg
    23701 ttcaccgcag ctggctggat cgcgacgaga aagtagtggt catgcaaacc gtgcgtgatc
    23761 agattggcca gcacgttttt accgctcatc gtctggaccg acccacttct ggtgtgttgt
    23821 tgatgggatt atccagcgag gccggacggc tgctggcaca acagtttgaa cagcaccaaa
    23881 tccagaaacg ttaccatgcg attgtgcgcg gctggttgat ggaagaagca gttctggatt
    23941 atccactggt ggaagaactg gacaaaatcg ctgataaatt tgcccgcgaa gataaaggct
    24001 cgcagccagc agtgacgcat tatcgcggtc tggcgaccgt agaaatgccg gtagcgaccg
    24061 gacgttaccc gaccacgcgc tacggcctgg tggaactgga gccgaaaacc ggacgcaaac
    24121 atcagcttcg ccgacatctt gcccatttgc gtcatccgat tattggcgat agcaaacatg
    24181 gcgatttacg ccagaatcgc agcggtgctg agcattttgg cctccagcgg ttaatgctac
    24241 atgccagtca gttgtcattg acgcatcctt ttactggcga gccgctgact attcacgcgg
    24301 gcctggacga cacctggatg caggcgttat cacaatttgg ctggcgcggt ctgcttcctg
    24361 aaaatgaaag ggttgagttc agtgcgccat cgggtcagga tggagagata agttcctaat
    24421 ccagggagaa aacggtatgg cggaaattgg tatttttgtc ggcaccatgt acggaaattc
    24481 gctgttagtg gcggaagaag cagaagcaat tctgaccgca cagggccaca aagcaacggt
    24541 atttgaagat cctgaattaa gcgactggct gccctatcag gataagtatg ttctggtggt
    24601 tacgtccacg accgggcagg gcgaccttcc tgatagcatt gtgccgctct ttcagggaat
    24661 caaagatagt ctgggcttcc agccgaatct gcgttatggc gtgattgcgc tcggcgacag
    24721 cagttatgta aatttctgca atggcggcaa acagttcgat gccttgttgc aggaacagag
    24781 cgctcagcgg gttggcgaaa tgctgttgat tgacgccagt gagaacccgg aaccggaaac
    24841 ggaatcaaat ccgtgggtcg aacagtgggg cacgctgttg tcctgatatg ttcagcgagc
    24901 ggtaaatgtc gttttagcgg tgctgaatcg aatattttca ggcaaatgcc agtaaaaact
    24961 gcttcatagc gcggattttt actggcgttt gcctggagtc aagcgatcca tttcatactc
    25021 ttctttattt cttcgtttta acccttcctt tcttgttctt gttttcattt ccgtgaagtg
    25081 gattccaccg tccagggcta atgccaaaat cgggcctcat tgaacgcatt aatgttgtgt
    25141 tgttgcacgg tgagccgcta tggcgcgctt tttatactgc tattgccaga tataaacacg
    25201 cgccgtattc ggcgaacgac ctataaaaac ggcaaaaaac accctacgtc acctctgatt
    25261 tcctggctat gtcgcagtcc agagtgagcg tggctaacgc gaattttcag gagtgcaaca
    25321 atgagttctt taagtcaggc tgcgagcagt gtggaaaaac gcacgaatgc tcgttactgg
    25381 atagtggtga tgttgtttat cgtcacatcc ttcaactacg gcgaccgcgc tacgctttct
    25441 attgccggtt cggaaatggc caaagatatc ggccttgatc ccgtgggaat gggctatgtg
    25501 ttctctgcct tctcatgggc ttatgttatc gggcagatcc ctggtggttg gttgctggat
    25561 cgttttggtt caaaacgcgt ctacttctgg tcgatcttta tctggtcgat gtttaccttg
    25621 ctgcaaggct tcgtcgatat ctttagtgga ttcggcatta tcgttgccct gtttactctg
    25681 cgcttcctgg tcgggcttgc tgaagcgcca tctttccccg gcaacagtcg cattgttgcg
    25741 gcctggtttc cggcgcagga aaggggaacg gcggtgtcga tttttaactc cgctcaatac
    25801 ttcgcaacgg tgatcttcgc gccgattatg ggctggctga cgcatgaagt gggctggtca
    25861 cacgtcttct tctttatggg cggtctgggg attgtcatca gctttatctg gttgaaagtc
    25921 atccacgagc caaatcaaca tccgggggta aatcagaaag agctggagta catcgccgcg
    25981 ggtggcgcgc tgatcaatat ggatcagcaa aacaccaaag ttaaagtgcc gttcagcgtg
    26041 aagtgggggc agatcaaaca actgctcggg tcacggatga tgatcggcgt ttatatcggt
    26101 cagtactgta tcaacgccct gacttacttt ttcattacct ggtttccggt ttatctggtg
    26161 caggcgcgtg ggatgtcgat tctgaaagcg ggctttgtgg cttccgttcc ggcggtttgc
    26221 ggttttatcg gcggtgtgct gggtgggatt atttccgact ggctgatgcg ccgcacggga
    26281 tcgctgaaca ttgcgcgtaa aacaccgatc gtaatgggca tgttgctgtc gatggtgatg
    26341 gtgttctgca actacgtcaa cgttgagtgg atgatcatcg gctttatggc gctggccttc
    26401 ttcggtaagg gcatcggggc gctgggttgg gcagtaatgg cagataccgc gccaaaagag
    26461 atcagcggtc tttccggtgg cctgttcaac atgttcggta acatttctgg catcgtcacg
    26521 ccaatcgcaa ttggttatat cgttggcacg actggctcct ttaatggggc gctgatttat
    26581 gttggtgttc atgccttaat cgcggtactg agctacctgg tgctggtggg cgatatcaag
    26641 cgtatcgagc tgaaacctgt cgcggggcaa taagatgacg acacaatcca gtcctgttat
    26701 tactgatatg aaagtcattc cggtcgccgg gcacgatagc atgttgctta atattggtgg
    26761 ggcacataac gcatatttca cccgcaatat tgtggtactc accgataacg ccgggcatac
    26821 cggcattggt gaagcgccgg gcggagaggt gatttatcaa acactggtcg atgctattcc
    26881 gatggttctg ggccaggaag ttgcgcgcct gaataaagtg gttcagcagg tgcataaagg
    26941 taatcaggca gccgattttg ataccttcgg caaaggtgcc tggacttttg aattgcgcgt
    27001 taatgccgtg gcggcgctgg aagccgcctt gcttgacctg ctaggtaagg cgctgaatgt
    27061 tccggtctgc gaactgttgg ggccaggcaa gcaacgcgag actattaccg tcctcggtta
    27121 tctgttttat atcggtgatc ggaccaaaac cgatcttcct tatctggaaa atacgccggg
    27181 caaccatgag tggtatcagt tgcgccatca gaaagcgatg aacagcgaag ccgttgtgcg
    27241 tctggcggaa gcctcacagg atcgctatgg ctttaaagat ttcaaactta agggcggggt
    27301 gttacctggc gagcaagaaa tcgacactgt tcgtgcattg aagaaacgct tccctgatgc
    27361 gcggattacc gttgatccca acggtgcctg gctacttgat gaagccattt ctctatgcaa
    27421 agggctgaat gatgttctta cctatgccga agatccatgc ggcgcagaac agggtttctc
    27481 cggtcgtgaa gttatggcgg agtttcggcg ggcgaccggc ttgcccgtcg cgactaacat
    27541 gatcgccacc aactggcgcg aaatgggtca tgcggtgatg ctcaatgcgg tagatattcc
    27601 acttgccgat ccgcacttct ggacgctttc cggtgcagtc cgtgtggcgc agctttgcga
    27661 cgactggggg ctgacctggg gctgccattc taataaccat ttcgatatct ctctggcgat
    27721 gtttacccat gtgggcgcgg cggcaccggg taatcctacc gctatcgata cccactggat
    27781 ttggcaggag ggcgattgtc gcctgaccaa aaatccgctg gagattaaaa acggaaaaat
    27841 tgccgttcct gatgcgcccg gtctgggcgt ggaactggac tgggaacagg tacaaaaggc
    27901 acatgaggcc tataaacgtc tgcctgtcgg tgcgcgtaac gacgcaggtc cgatgcagta
    27961 cctgatcccc ggctggacct ttgaccgtaa acgtcccgtt ttcggccgtc attgattctg
    28021 aaaaaggaca taaatatgag ttctcaattt acgacgcctg ttgttactga aatgcaggtc
    28081 atcccggtgg cgggtcatga cagtatgctg atgaatctga gtggtgcaca cgcaccgttc
    28141 tttacgcgta atattgtgat tatcaaagat aattctggtc acactggcgt aggggaaatt
    28201 cccggcggcg agaaaatccg taaaacgctg gaagatgcga ttccgctggt ggtaggtaaa
    28261 acgctgggtg aatacaaaaa cgttctgacg ctggtgcgta atacttttgc cgatcgtgat
    28321 gctggtgggc gcggtttgca gacatttgat ttgcgtacca ctattcatgt agttaccggg
    28381 atagaagcgg caatgctgga tctgctgggg cagcatctgg gggtaaacgt ggcatccctg
    28441 ctgggcgatg gtcaacagcg tagcgaagtc gaaatgctcg gttatctgtt cttcgtcggt
    28501 gatcgcaaag ccacaccgct gccgtatcaa agccagccgg atgactcatg cgactggtat
    28561 cgcctgcgtc atgaagaagc gatgacgccg gatgcggtgg tgcgcctggc ggaagcggcg
    28621 tatgaaaaat atggcttcaa cgatttcaaa ctgaaaggcg gtgtactggc cggggaagaa
    28681 gaggccgagt ctattgtggc actggcgcaa cgcttcccgc aggcgcgtat tacgctcgat
    28741 cctaacggtg cctggtcgct gaacgaagcg attaaaattg gtaaatacct gaaagggtcg
    28801 ctggcttatg cagaagatcc gtgtggtgcg gagcaaggtt tctccgggcg tgaagtgatg
    28861 gcagagttcc gtcgcgcaac tggcctgccg accgcaacca atatgatcgc caccgactgg
    28921 cggcagatgg gccatacgct ctccctgcaa tccgttgata tcccgctggc ggatccgcat
    28981 ttctggacga tgcaaggttc ggtacgtgtg gcgcaaatgt gccatgaatt tggcctgacc
    29041 tggggttcac actcaaacaa ccacttcgat atttccctgg cgatgtttac ccatgttgcc
    29101 gccgctgcac cgggtaaaat tactgctatt gatacgcact ggatctggca ggaaggtaat
    29161 cagcgcctga ccaaagaacc gtttgagatc aaaggcgggc tggtacaggt gccagaaaaa
    29221 ccggggctgg gtgtagaaat cgatatggat caagtgatga aagcccatga gctgtatcag
    29281 aaacacgggc ttggcgcgcg tgacgatgcg atgggaatgc agtatctgat tcctggctgg
    29341 acgttcgata acaagcgccc gtgcatggtg cgttaagaca gaaaaccaaa accggctcta
    29401 tctggagccg gcttgagact gatgctaaac gcaaaatagc ctgttgtgct atgtatcagg
    29461 tctacatcat tttttaattc attgaattta attggttttg taggccggat aaggcgttaa
    29521 cgccgcatcc ggcataaaca caggcacttt gtcaccaatc tgaaaccagc gtcataaaaa
    29581 gccgattgct actcgacaag acgtccgtta cccgagaatt ttgctggctt cgcgcgctac
    29641 attatccatc tcgtccaaca gttccagcag ctccggctcc aaatcttctt ctttagtacc
    29701 gctacgtagc tgttgttcga tcagttggca gagattcttc atacgcggca caccgctata
    29761 gccgcaactg ccatgcagtt tatgaatcaa atcaaccagg ccttccgggt tttctccaac
    29821 cagctgttcc tcaactttgt tgcgaacttc aggcaggaaa tcgagtaaca tttgcagcat
    29881 atcgcgcgct aaatcggttt ttcctgctgc ctggcgtagt gccagttgcc agtcgagggt
    29941 cgcattcggg ttcaccacaa tttcgttgac ttcgggcgtc acgacgcgag aggaaatacc
    30001 gctgccaggc ttgtagcgca acaacaaatt atgcaatcgc tcttcttcaa tcggtttcgc
    30061 cagataatcg ctcatccctg cgccaagcag cttctctttt tgcccggcca ttgcatgcgc
    30121 cgttaccgcg ataaccggcg tttgttgctg atgggggagc tggtggatga gctcgcaggc
    30181 ccgaatgcca tccatgtcag gcatttgaat atccattaag atcaaatcga acggcatctg
    30241 tttcgcccgt tcaaccgcct gatgcccgct atcgcaaagt tccacatgtt gcaccatatc
    30301 ttccagcaat gcgccgataa gtttcaggtt agcggggttg tcatcaactg ccatgactgt
    30361 cattgccagc ttgctttcat cggttacagg caaaagcgtg ttttgtttgt gatgacaaaa
    30421 ttccgtcagg gcaggcaaca ggcgcgtagg tgttaatggt ttcagcagac acgcgccgat
    30481 accatcttgc ttgagttttt ctgcattgac ttgtgcatgg caaggaagtg ccagcatcag
    30541 gaaatcggtc atcgataccg ctttcgctaa tcgctcatgt tgcattgtta gcggctcgcg
    30601 gaaggtcacc gcgatgccta acaacatcat gtcgtaatgc gcgggaggca gcgcggagaa
    30661 cgttgggcta taaaccactt ccagcggcgt ttcacttaaa atatccagcg tgcattgcgc
    30721 tgctgcggag tttggttcga cataggccag gcgcttaccg gcgaggcact gggtggatgg
    30781 cccttcgata ataatgttcg ggttcagatc gagattaatg tggaaccaga aagttgaacc
    30841 gcgattcggc tggctatgga acgaaatatc gccgcccatt tcattaacca gtttttgtgt
    30901 aatcaccagc cccagaccgg tgccaccatg acggcgggaa atactggcat cagcctgtcg
    30961 gaaggcctgg aataagcgcg attgatcgcg ttcaggaata ccaatgccgg tatcccgaat
    31021 ctgcacttca atctgcactt tggtattact cagcgcacgt ttttctacca gaatatcaat
    31081 gttgccattc tcggtgaatt taattgcatt ccccaccagg ttagtgatga tttgctgtaa
    31141 tcgcagtggg tcgccgatca cgttatcagg cacgtcgctt ttaatattga gcgtcagttc
    31201 caacccttta tcgtgagaag aatgtgccag cagagtaacg acttcatcca gcgtgctgcg
    31261 tagtgggaat ggaatacttt ccagaatcag cttacctgct tccagtttcg agaagtcgag
    31321 aacatcatta ataattgcca gcaaattatt tgccgaacgt tcaatcgtat tcaggtgatc
    31381 gcgctgcgtt ggtgttaatt ctgttttcag cgtcaggcgg gtaaagccaa taacaccatt
    31441 cagtggtgta cgcagctcgt gtgacatatt tgccagaaac tcggatttaa tacgcgccgc
    31501 ttcctgggcg cgctttttcg ccagatctaa ctcaacgttc tgaatttcca tctgctccag
    31561 cgtttcacgc agatcggacg tcgcctggtc gatattgtgc tgcatctctt cgtgataagc
    31621 agccagcgac attgccatcg agttgatacc gtttttcagc atatccagct cgccgagcat
    31681 aaatccttcc acccggctgt cgagttgccc gcgacggatg cggtcgacgg tattcaccat
    31741 gttgcgaatc ggaccggtta catcgcgcat taagcgccag ccaaaaataa gcgcaatacc
    31801 gatacaaaac agcatcatca cgctggaaat aaagatctct ttatattgct gcaagcgaac
    31861 cgatttaaga tccagctcca gcgcaatata tcccaacata ttttgactat ttttggcatc
    31921 gctactgggc gattcgtcgg gggagtaact ctcagaaata atcggcgtgc ggaggatcat
    31981 aatatcgccg tcacgagtga cagtgagctg gcgaggaaac ggcacgttgc tgccgagctg
    32041 cattgatgag ggatcaagat gaaaattgga ggtgacaaag agtcggttat tttcatcata
    32101 aaccgaaatc gcgcgaacaa tatcggaatg gcgacgatgc agtacgctta ttaactgacc
    32161 gatagattcg cgattttgca ggctcatgcc atattcagta gaaactgcaa gcggctcaat
    32221 aatgctggca ccggcatctt ccagttgacg ctgcaagtcg ttatagcgat gcacgacgaa
    32281 aaagatactc agcaataaac caataaggac ggtcggtgcc aggatcagaa tcatcatgcg
    32341 tgcgcgcagg ctgtagttgg tcatggagtt ccgttatggg acaattaagg tcacactgtt
    32401 aaattgagaa agtctcgcca atggcgcaat tctactctgc aaaacgacgc acgacgacgc
    32461 gtcagatcat aaccgtttca gtcaacgacc tcgactcttt tggtcagggc gtggcgcgac
    32521 ataacggcaa aacgttattt atccccggat tattgccgca ggaaaacgcg gaagttgctg
    32581 ttactgaaga taaaaaacag tatgcccgcg ctaaagtcgt acgccggtta agcgatagcc
    32641 cggaacgcga aacaccacgc tgtcctcatt ttggcgtatg cggcggctgt cagcaacaac
    32701 acgccagcgt ggatttacag cagcgaagca aaagtgcggc actcgcccga ttaatgaaac
    32761 acgatgtctc tgaagtgatc gccgatgttc cctggggcta tcgccgccgc gcgcgtttaa
    32821 gtctgaacta cttaccgaaa acacagcaac ttcagatggg gtttcgcaaa gcgggctcca
    32881 gtgacattgt cgacgttaaa caatgcccca ttttagtgcc ccaacttgaa gcattgctgc
    32941 ccaaagtcag ggcatgcctg ggcagcttac aagttatgcg ccatcttggt catgttgaac
    33001 tggtacaggc aaccagcggc acgctgatga ttttgcgcca taccgcaccg ctaagttcgg
    33061 cagatcgcga aaaactggaa cgcttttcgc attctgaagg cctggatctg tatctcgccc
    33121 ccgatagtga gatactcgaa accgtctctg gtgagatgcc ctggtatgac tcaaacgggt
    33181 tgcgcttaac ttttagcccg cgcgatttta ttcaggtcaa tgcgggtgtg aaccaaaaaa
    33241 tggtagcgcg tgcgttggaa tggctggatg tgcaacctga agatcgcgta ctggatctgt
    33301 tctgcggtat gggcaacttt acactgccat tggcgaaaca agcagccagt gtggtcggtg
    33361 tagaaggtgt tccggcgctg gtggaaaaag gccagcagaa tgcgcgtctt aatggtttac
    33421 agaatgtgac gttttatcac gaaaatcttg aagaagatgt cacaaagcag ccgtgggcga
    33481 aaaacggctt cgataaagtg ttgctggacc cggcgcgagc aggtgccgca ggtgttatgc
    33541 agcaaattat aaaactggaa cctattcgta tagtttatgt atcctgtaac cctgcaacgc
    33601 tggctcggga tagcgaagcg ttattaaaag caggatatac cattgcgcga ctggcgatgc
    33661 tggatatgtt cccacacacg ggacatctgg aatcgatggt acttttctcg cgcgttaaat
    33721 agttacgatt tgccgatttc ggcaggtctg gtccctaaag gagaggacaa tggttgcggt
    33781 aagaagtgca catatcaata aggctggtga atttgatccg gaaaaatgga tcgcaagtct
    33841 gggtattacc agccagaagt cgtgtgagtg cttagccgaa acctgggcgt attgtctgca
    33901 acagacgcag gggcatccgg atgccagtct gttattgtgg cgtggtgttg agatggtgga
    33961 gatcctctcg acattaagta tggacattga cacgctgcgg gcggcgctgc tgttccctct
    34021 ggctgatgcc aacgtagtca gcgaagatgt gctgcgtgag agcgtcggta agtcggtcgt
    34081 taaccttatt cacggcgtgc gtgatatggc ggcgatccgc cagctgaaag cgacgcacac
    34141 tgattctgtt tcctccgaac aggtcgataa cgttcgccgg atgttattgg cgatggtcga
    34201 tgattttcgc tgcgtggtca tcaaactggc ggagcgtatt gctcatctgc gcgaagtaaa
    34261 agatgcgccg gaagatgaac gcgtactggc ggcaaaagag tgcaccaata tctacgcgcc
    34321 gttggcgaac cgtcttggga ttgggcaact gaaatgggag ctggaagatt actgcttccg
    34381 ttatctccac ccgaccgaat acaaacgcat cgcaaaactg ctgcatgaac gccgtctcga
    34441 ccgcgaacac tatatcgaag agtttgtcgg ccatctgcgc gctgagatga aagctgaagg
    34501 cgttaaagct gaagtgtatg gtcgtccgaa acacatctac agtatctggc gcaaaatgca
    34561 gaaaaagaac ctcgccttcg atgagctgtt tgatgtgcgt gcggtacgta ttgtcgccga
    34621 gcgtttacag gattgttatg ccgcactggg gatagtgcac actcactatc gccacctgcc
    34681 ggatgagttt gacgattatg tcgctaaccc gaaaccaaac ggttatcagt ctattcatac
    34741 cgtggttctg gggccaggtg gcaaaaccgt tgagatccaa atccgcacca aacagatgca
    34801 tgaagatgca gagttgggtg ttgctgcgca ctggaaatat aaagagggcg cggcagctgg
    34861 cggcgcacgt tcgggacatg aagaccggat tgcctggctg cgtaaactga ttgcgtggca
    34921 ggaagagatg gctgattccg gcgaaatgct cgacgaagta cgcagccagg tctttgacga
    34981 ccgggtgtat gtctttacgc cgaaaggtga tgtcgttgat ttgcctgcgg gatcaacgcc
    35041 gctggacttc gcttaccaca tccacagtga tgtcggacac cgctgcatcg gggcaaaaat
    35101 tggcgggcgc attgtgccgt tcacctacca gctgcagatg ggcgaccaga ttgaaattat
    35161 cacccagaaa cagccgaacc ccagccgtga ctggttaaac ccaaacctcg gttacgtcac
    35221 aaccagccgt gggcgttcga aaattcacgc ctggttccgt aaacaggacc gtgacaaaaa
    35281 cattctggct gggcggcaaa tccttgacga cgagctggaa catctgggga tcagcctgaa
    35341 agaagcagaa aaacatctgc tgccgcgtta caactttaat gatgtcgacg agttgctggc
    35401 ggcgattggt ggcggggata tccgtctcaa tcagatggtg aacttcctgc aatcgcaatt
    35461 taataagccg agtgccgaag agcaggacgc cgccgcgctg aagcaacttc agcaaaaaag
    35521 ctacacgccg caaaaccgca gtaaagataa cggtcgcgtg gtggtcgaag gtgttggcaa
    35581 cctgatgcac cacatcgcgc gctgctgcca gccgattcct ggagatgaga ttgtcggctt
    35641 cattacccag gggcgcggta tttcagtaca ccgcgccgat tgcgaacaac tggcggaact
    35701 gcgctcccat gcgccagaac gcattgttga cgcggtatgg ggtgagagct actccgccgg
    35761 atattcgctg gtggtccgtg tggtggctaa tgatcgtagt gggttgttac gtgatatcac
    35821 gaccattctc gccaacgaga aggtgaacgt gcttggcgtt gccagccgta gcgacaccaa
    35881 acagcaactg gcgaccatcg acatgaccat tgagatttac aacctgcaag tgctggggcg
    35941 cgtgctgggt aaactcaacc aggtgccgga tgttatcgac gcgcgtcggt tgcacgggag
    36001 ttaggccgaa atttgctcgt atctacaatg tagattgata tatactgtat ctacatatga
    36061 tagcggtttg aggaaagggt tatgatccac agtagcgtaa agcgttgggg aaattcaccg
    36121 gcggtgcgga tcccggctac gttaatgcag gcgctcaatc tgaatattga tgatgaagtg
    36181 aagattgacc tggtggatgg caaattaatt attgagccag tgcgtaaaga gcccgtattt
    36241 acgcttgctg aactggtcaa cgacatcacg ccggaaaacc tccacgagaa tatcgactgg
    36301 ggagagccga aagataagga agtctggtaa tggtaagccg atacgtaccc gatatgggcg
    36361 atctgatttg ggttgatttt gacccgacaa aaggtagcga gcaagctgga catcgtccag
    36421 ctgttgtcct gagtcctttc atgtacaaca acaaaacagg tatgtgtctg tgtgttcctt
    36481 atacaacgca atcaaaagga tatccgttcg aagttgtttt atccggtcag gaacgtgatg
    36541 gcgtagcgtt agctgatcag gtaaaaagta tcgcctggcg ggcaagagga gcaacgaaga
    36601 aaggaacagt tgccccagag gaattacaac tcattaaagc caaaattaac gtactgattg
    36661 ggtagtgtta ctaactcact tctattctgg tcacaggttt ccacctgaca gacccgcaat
    36721 tttcaggaca attcaatgaa tcaaatcgac cgtttgctca ctattatgca gcgcctgcgc
    36781 gatccggaaa acggctgccc gtgggataaa gagcagacat ttgccaccat tgcgccttac
    36841 acccttgaag aaacctacga agtgctggac gccatcgccc gtgaagattt tgacgatctt
    36901 cgcggtgaac tgggcgatct gctattccag gtggtgtttt acgcgcaaat ggctcaggaa
    36961 gaagggcgct ttgactttaa tgatatttgc gctgctatta gcgataaatt agagcgtcgc
    37021 catccgcatg tttttgctga tagttctgcc gaaaacagta gtgaagtgct tgcccgttgg
    37081 gagcaaatca aaaccgaaga gcgcgcgcag aaagcgcagc attcggcgct ggacgatatt
    37141 cctcgtagtt taccggcttt aatgcgtgcg caaaaaatcc agaaacgttg cgccaacgtt
    37201 ggcttcgatt ggacgacgct tggtccggta gtcgataaag tctacgaaga gatcgacgag
    37261 gtgatgtacg aagcgcggca ggctgttgtc gaccaggcta aactggagga ggaaatgggg
    37321 gacctgctgt ttgccacggt caatctggct cgtcatttag ggacaaaagc ggaaatcgca
    37381 ttgcaaaaag cgaacgaaaa atttgagcgt cgttttcgcg aagtggagcg tattgttgcc
    37441 gcgcgtggac tggaaatgac aggtgttgac ctcgaaacaa tggaagaagt ctggcaacag
    37501 gtaaaacggc aggaaattga tctctaaggg aattacgcgg tcaagcgcca tttgtgtcat
    37561 tttttaaatg acaagcgctt gatttgcgtc aaaaacattt accccaaagg ggctattttc
    37621 tcactcctga tttcaatagt gcgctggcga agaggaggga taatgaaagt ttgtggcaca
    37681 ggtcatgttc gggtatactg ctttcccgtc ctggttattc catcgtcttt tcaacctaac
    37741 ttctcaggtt cagcatgaca acgaactata tttttgtgac cggcggggtc gtatcctctc
    37801 tgggtaaagg cattgccgca gcctccctcg cagccattct tgaagcccgt ggcctcaatg
    37861 tgaccatcat gaaactggat ccgtacatca acgtcgatcc aggtactatg agcccaatcc
    37921 aacacgggga agtgttcgtt actgaagacg gcgctgaaac cgacctggac ctggggcact
    37981 acgagcgttt cattcgtacc aaaatgagcc gccgcaacaa cttcaccacg ggtcgtatct
    38041 actctgacgt tctgcgtaaa gaacgccgcg gtgactacct cggcgcaacc gtgcaggtta
    38101 ttccgcacat cactaacgca atcaaagagc gcgtgctgga aggtggcgaa ggtcatgacg
    38161 tagtactggt agaaatcggc ggtacagtag gtgatatcga atccttgccg ttcctcgaag
    38221 cgattcgcca gatggctgtt gaaattggcc gtgagcacac tctgtttatg cacctgacgc
    38281 tggtgccgta catggcagcg tctggtgaag tcaaaaccaa accgactcag cactctgtaa
    38341 aagagctgct ctccatcggt atccagcctg acatcctgat ttgtcgttca gatcgcgctg
    38401 ttccggcgaa cgaacgtgcg aagattgcat tgttctgtaa tgttccggaa aaagcggtta
    38461 tttctctgaa agacgtcgat tccatctata aaattccggg cctgttgaaa tctcaggggc
    38521 tggacgatta tatttgtaaa cgattcagct taaactgccc ggaagcgaat ctgtccgaat
    38581 gggaacaggt tatcttcgaa gaagcgaatc cggtaagtga agtcaccatc ggtatggtcg
    38641 gcaagtacat tgaactgccg gatgcttata aatcagtgat cgaagcactg aaacacggtg
    38701 ggctgaagaa tcgtgtcagc gtcaacatca aactgatcga ttcacaagat gttgaaacgc
    38761 gcggcgttga aatccttaaa ggtctggacg caatcctcgt acctggcggt tttggctatc
    38821 gtggcgtaga aggcatgatt acgaccgcgc gttttgcgcg tgagaacaat attccttatc
    38881 tgggcatttg cctgggtatg caggtggcgt taattgatta cgctcgccat gttgccaaca
    38941 tggagaacgc caactctacg gaatttgtgc cagaatgtaa gtacccggtt gtggcgctga
    39001 ttaccgagtg gcgcgatgaa aacggcaacg ttgaagttcg tagcgagaag agcgatctcg
    39061 gcggaaccat gcgtctcggc gcacagcagt gccagttggt tgacgatagc ctggttcgcc
    39121 agctgtacaa tgcgccgaca attgttgagc gtcatcgtca ccgttacgaa gtcaacaaca
    39181 tgctgttgaa acagattgaa gatgcaggtc tgcgcgttgc gggccgttcc ggggatgatc
    39241 agttggtcga gatcatcgaa gttccgaatc acccgtggtt cgtggcttgc cagttccatc
    39301 cggagtttac ttctactcca cgtgatggtc acccgctgtt tgcaggcttt gtgaaagccg
    39361 ccagcgagtt ccagaaacgt caggcgaagt aagtaaaaaa gttagaacgg caacgcgtac
    39421 cctgggtacg cgttgtttgt ctggagtttc agtttaacta gtgacttgag gaaaacctaa
    39481 tgtccaaaat cgtaaaaatc atcggtcgtg aaatcatcga ctcccgtggt aacccgactg
    39541 ttgaagccga agtacatctg gagggtggtt tcgtcggtat ggcagctgct ccgtcaggtg
    39601 cttctactgg ttcccgtgaa gctctggaac tgcgcgatgg cgacaaatcc cgtttcctgg
    39661 gtaaaggcgt aaccaaagct gttgctgcgg taaacggccc gatcgctcag gcgctgattg
    39721 gcaaagatgc taaagatcag gctggcattg acaagatcat gatcgacctg gacggcaccg
    39781 aaaacaaatc caaattcggc gcgaacgcaa tcctggctgt atctctggct aacgccaaag
    39841 ctgctgcagc tgctaaaggg atgccgctgt acgagcacat cgctgaactg aacggtactc
    39901 cgggcaaata ctctatgccg gttccgatga tgaacatcat caacggtggt gagcacgctg
    39961 acaacaacgt tgatatccag gaattcatga ttcagccggt tggcgcgaaa actgtgaaag
    40021 aagccatccg catgggttct gaagttttcc atcacctggc aaaagttctg aaagcgaaag
    40081 gcatgaacac tgctgttggt gacgaaggtg gctatgcgcc gaacctgggt tccaacgctg
    40141 aagctctggc tgttatcgct gaagctgtta aagctgctgg ttatgaactg ggcaaagaca
    40201 tcactctggc gatggactgc gcagcttctg aattctacaa agacggtaaa tacgttctgg
    40261 ctggcgaagg caacaaagcg ttcacctctg aagaattcac tcacttcctg gaagaactga
    40321 ccaaacagta cccgatcgtt tctatcgaag acggtctgga cgaatctgac tgggacggtt
    40381 tcgcatacca gaccaaagtt ctgggcgaca aaatccagct ggttggtgac gacctgttcg
    40441 taaccaacac caagatcctg aaagaaggta tcgaaaaagg tatcgctaac tccatcctga
    40501 tcaaattcaa ccagatcggt tctctgaccg aaactctggc tgcaatcaag atggcgaaag
    40561 atgctggcta cactgcagtt atctctcacc gttctggcga aactgaagac gctaccatcg
    40621 ctgacctggc tgttggtact gctgcaggcc agatcaaaac tggttctatg agccgttctg
    40681 accgcgttgc taaatacaac cagctgattc gtatcgaaga agctctgggc gaaaaagcac
    40741 cgtacaacgg tcgtaaagag atcaaaggcc aggcataaga ctgactttat ctgatttaaa
    40801 aatgccagcc tccgggctgg cattttttat ggttgagtta ccagcggccg gatgcgccgc
    40861 cacctccaga agaaccgcca ccgccgctaa atccgccgcc acttgagcct ccaccggaac
    40921 cgctgttgtc tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg
    40981 taaagaccat aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt
    41041 tatcggagaa caggaataaa tctaaaagat agattgctga aatatagact ccacttttac
    41101 atgcgcgaca aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa
    41161 atacccaaaa aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag
    41221 gattagtcgg atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct
    41281 ctaatccctg agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat
    41341 ctctagccag agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag
    41401 tacgatctga ccaggcaaca atgatcagta tcccatcatt acgtttggca tctcctaaac
    41461 gccaattgtc aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa
    41521 caattaatac agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca
    41581 gttctttctt ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tactgtaatt
    41641 gtgggacaat agttggttgg gctgcaacaa aggaacatac ga
//
LOCUS       DAZGCS010000035        41545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGCS010000035 DAZGCS010000000
VERSION     DAZGCS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.035"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1390)
                     /gene="rhsB"
                     /locus_tag="ACHK01_001918"
     CDS             complement(<1..1390)
                     /gene="rhsB"
                     /locus_tag="ACHK01_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO8096877.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGRTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDS
                     LDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRA"
     gene            complement(1550..1762)
                     /gene="rpmE"
                     /locus_tag="ACHK01_001919"
     CDS             complement(1550..1762)
                     /gene="rpmE"
                     /locus_tag="ACHK01_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO8096878.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1965..4163
                     /gene="priA"
                     /locus_tag="ACHK01_001920"
     CDS             1965..4163
                     /gene="priA"
                     /locus_tag="ACHK01_001920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO8096879.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKEQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4319..5344
                     /gene="cytR"
                     /locus_tag="ACHK01_001921"
     CDS             4319..5344
                     /gene="cytR"
                     /locus_tag="ACHK01_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO8096880.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5436..6395
                     /gene="ftsN"
                     /locus_tag="ACHK01_001922"
     CDS             5436..6395
                     /gene="ftsN"
                     /locus_tag="ACHK01_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO8096881.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQADTVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6488..7018
                     /gene="hslV"
                     /locus_tag="ACHK01_001923"
     CDS             6488..7018
                     /gene="hslV"
                     /locus_tag="ACHK01_001923"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO8096882.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7028..8359
                     /gene="hslU"
                     /locus_tag="ACHK01_001924"
     CDS             7028..8359
                     /gene="hslU"
                     /locus_tag="ACHK01_001924"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO8096883.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            8426..9352
                     /gene="menA"
                     /locus_tag="ACHK01_001925"
     CDS             8426..9352
                     /gene="menA"
                     /locus_tag="ACHK01_001925"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO8096884.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            9445..9930
                     /gene="rraA"
                     /locus_tag="ACHK01_001926"
     CDS             9445..9930
                     /gene="rraA"
                     /locus_tag="ACHK01_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO8096885.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(10015..10260)
                     /gene="zapB"
                     /locus_tag="ACHK01_001927"
     CDS             complement(10015..10260)
                     /gene="zapB"
                     /locus_tag="ACHK01_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO8096886.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10685..11530
                     /gene="glpF"
                     /locus_tag="ACHK01_001928"
     CDS             10685..11530
                     /gene="glpF"
                     /locus_tag="ACHK01_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO8096887.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11553..13061
                     /gene="glpK"
                     /locus_tag="ACHK01_001929"
     CDS             11553..13061
                     /gene="glpK"
                     /locus_tag="ACHK01_001929"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO8096888.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13197..14207
                     /gene="glpX"
                     /locus_tag="ACHK01_001930"
     CDS             13197..14207
                     /gene="glpX"
                     /locus_tag="ACHK01_001930"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8096889.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14304..15050
                     /gene="fpr"
                     /locus_tag="ACHK01_001931"
     CDS             14304..15050
                     /gene="fpr"
                     /locus_tag="ACHK01_001931"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO8096890.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15055..15483)
                     /gene="uspD"
                     /locus_tag="ACHK01_001932"
     CDS             complement(15055..15483)
                     /gene="uspD"
                     /locus_tag="ACHK01_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO8096891.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(15510..15809)
                     /gene="yiiS"
                     /locus_tag="ACHK01_001933"
     CDS             complement(15510..15809)
                     /gene="yiiS"
                     /locus_tag="ACHK01_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO8096892.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(16021..16461)
                     /gene="yiiR"
                     /locus_tag="ACHK01_001934"
     CDS             complement(16021..16461)
                     /gene="yiiR"
                     /locus_tag="ACHK01_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8096893.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16562..17161
                     /gene="yiiQ"
                     /locus_tag="ACHK01_001935"
     CDS             16562..17161
                     /gene="yiiQ"
                     /locus_tag="ACHK01_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO8096894.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17269..18036
                     /gene="tpiA"
                     /locus_tag="ACHK01_001936"
     CDS             17269..18036
                     /gene="tpiA"
                     /locus_tag="ACHK01_001936"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO8096895.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(18091..18846)
                     /gene="cdh"
                     /locus_tag="ACHK01_001937"
     CDS             complement(18091..18846)
                     /gene="cdh"
                     /locus_tag="ACHK01_001937"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO8096896.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18953..19942)
                     /gene="sbp"
                     /locus_tag="ACHK01_001938"
     CDS             complement(18953..19942)
                     /gene="sbp"
                     /locus_tag="ACHK01_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO8096897.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20262..21224)
                     /gene="pfkA"
                     /locus_tag="ACHK01_001939"
     CDS             complement(20262..21224)
                     /gene="pfkA"
                     /locus_tag="ACHK01_001939"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO8096898.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21405..22307)
                     /gene="fieF"
                     /locus_tag="ACHK01_001940"
     CDS             complement(21405..22307)
                     /gene="fieF"
                     /locus_tag="ACHK01_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO8096899.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22456..22956)
                     /gene="cpxP"
                     /locus_tag="ACHK01_001941"
     CDS             complement(22456..22956)
                     /gene="cpxP"
                     /locus_tag="ACHK01_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO8096900.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            23106..23804
                     /gene="cpxR"
                     /locus_tag="ACHK01_001942"
     CDS             23106..23804
                     /gene="cpxR"
                     /locus_tag="ACHK01_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO8096901.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23801..25174
                     /gene="cpxA"
                     /locus_tag="ACHK01_001943"
     CDS             23801..25174
                     /gene="cpxA"
                     /locus_tag="ACHK01_001943"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO8096902.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25280..25954)
                     /gene="yiiM"
                     /locus_tag="ACHK01_001944"
     CDS             complement(25280..25954)
                     /gene="yiiM"
                     /locus_tag="ACHK01_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO8096903.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(26103..27086)
                     /gene="kdgT"
                     /locus_tag="ACHK01_001945"
     CDS             complement(26103..27086)
                     /gene="kdgT"
                     /locus_tag="ACHK01_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO8096904.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27346..27966)
                     /gene="sodA"
                     /locus_tag="ACHK01_001946"
     CDS             complement(27346..27966)
                     /gene="sodA"
                     /locus_tag="ACHK01_001946"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO8096905.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28251..29285
                     /gene="rhaT"
                     /locus_tag="ACHK01_001947"
     CDS             28251..29285
                     /gene="rhaT"
                     /locus_tag="ACHK01_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO8096906.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(29282..30130)
                     /gene="rhaR"
                     /locus_tag="ACHK01_001948"
     CDS             complement(29282..30130)
                     /gene="rhaR"
                     /locus_tag="ACHK01_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO8096907.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(30204..31040)
                     /gene="rhaS"
                     /locus_tag="ACHK01_001949"
     CDS             complement(30204..31040)
                     /gene="rhaS"
                     /locus_tag="ACHK01_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO8096908.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31328..32797
                     /gene="rhaB"
                     /locus_tag="ACHK01_001950"
     CDS             31328..32797
                     /gene="rhaB"
                     /locus_tag="ACHK01_001950"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO8096909.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            32794..34053
                     /gene="rhaA"
                     /locus_tag="ACHK01_001951"
     CDS             32794..34053
                     /gene="rhaA"
                     /locus_tag="ACHK01_001951"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO8096910.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            34295..35119
                     /gene="rhaD"
                     /locus_tag="ACHK01_001952"
     CDS             34295..35119
                     /gene="rhaD"
                     /locus_tag="ACHK01_001952"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO8096911.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            35129..35443
                     /gene="rhaM"
                     /locus_tag="ACHK01_001953"
     CDS             35129..35443
                     /gene="rhaM"
                     /locus_tag="ACHK01_001953"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO8096912.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35744..36190
                     /gene="frvA"
                     /locus_tag="ACHK01_001954"
     CDS             35744..36190
                     /gene="frvA"
                     /locus_tag="ACHK01_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO8096913.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            36201..37652
                     /gene="frvB"
                     /locus_tag="ACHK01_001955"
     CDS             36201..37652
                     /gene="frvB"
                     /locus_tag="ACHK01_001955"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO8096914.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37642..38712
                     /gene="frvX"
                     /locus_tag="ACHK01_001956"
     CDS             37642..38712
                     /gene="frvX"
                     /locus_tag="ACHK01_001956"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8096915.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            38712..40460
                     /gene="frvR"
                     /locus_tag="ACHK01_001957"
     CDS             38712..40460
                     /gene="frvR"
                     /locus_tag="ACHK01_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO8096916.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(40510..>41545)
                     /gene="yiiG"
                     /locus_tag="ACHK01_001958"
     CDS             complement(40510..>41545)
                     /gene="yiiG"
                     /locus_tag="ACHK01_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO8096917.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     PVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQG
                     PTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTIN
                     EMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKN
                     IEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGG
                     MNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYN
                     EMVDDYNSLR"
BASE COUNT         9984 a        10873 c        10803 g         9885 t
ORIGIN      
        1 atgccctgcc atccggcgtg gtgatgcgcg tgatgaggcc cgtcaccaca tccgggctgt
       61 actctgttgt ccggcctgcg gcatccgtct gtgccttaag cctgcccacc gcatcaaact
      121 gactctgcgt gacgctgccg tccgcgtgtt cctttttcac cacccgcttc agcccggctt
      181 cgccctgcgt gtgcagcact tcacggcggt ccaggctgtc ggtgatggtg atgtggtctt
      241 tctcatactg atacgtgtag cttaagcctg ccgggttaag ctgttctgtc acccgcccgt
      301 cgctgtcgta acggtaacgg atttccggcc ggcccgtgtg acggtgcgcc accatccggc
      361 cccggtattt atcatcgtaa gtaaagctgc gcacctgttt gccactacgg tcatacacca
      421 ccgccagttc gccgcgtggc gtccggccat agcgcaccag cggcgcggca ggtaaattct
      481 ccgggtattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg ttgtcccggc
      541 catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc ccgccggaaa
      601 tggcctgctg ccgggcttct tctgcccgct gcgcctgcgt ggtcagtacc agccggaagt
      661 gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcaccg gcggcttcgc
      721 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
      781 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
      841 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
      901 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacgccgc
      961 cgcgcaccag ccacagtgac tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
     1021 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
     1081 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
     1141 ttgtccggta actgctgtag gtgcgggaga ggatgaacgg cagcggggcg ggcagggcga
     1201 tgtcggtttc accgggaagg acctttgcac cgagcagagg attgaccgga tggccggacg
     1261 tcactccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccgatg cgcaccccgg
     1321 ctgaaccctg gacaatgcta ccgccatact gcgtcatgtc gccctgacgc gccgccggtt
     1381 ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa
     1441 caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat
     1501 tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
     1561 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
     1621 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
     1681 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     1741 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     1801 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
     1861 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     1921 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
     1981 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
     2041 ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaga gcaggagcgc atcggggttg
     2101 tggtgtcagt tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagtggaag
     2161 tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc tgggcggcag
     2221 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc
     2281 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggccagg
     2341 cggtggatct gaacagcctg aaacgctccc ccaagcaaca acaggcgctg gcggcgttac
     2401 ggcaaggcaa aatctggcgc gaccaggtcg ccacgctcga atttaatgat gccgcgttgc
     2461 aggcgctacg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
     2521 ggcaaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caggccaccg
     2581 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
     2641 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     2701 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
     2761 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcggc
     2821 tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtgatcggc acccgctccg
     2881 cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag cacgacagct
     2941 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc
     3001 atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttgtgca
     3061 acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg ccgggcgggg aatgcgcgtc
     3121 cggcaattca acacgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg
     3181 cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc ctctttctta
     3241 atcgtcgggg ttttgcgcct gcgctactgt gccacgactg cggctggatt gccgaatgcc
     3301 cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact
     3361 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtac
     3421 ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgctgttc cctgatgtgc
     3481 ccatttctcg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
     3541 cggaagtaca tcgcggcggt gcgaggattt tgatcggcac acaaatgctg gcgaaaggtc
     3601 accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg
     3661 ccgattttcg ctcggcagag cgtttcgctc agctttacac ccaggtcgct ggtcgtgccg
     3721 ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca ccatccagaa catcctctgt
     3781 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcgac
     3841 ggatgatgca actgccgcca tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     3901 atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctctcc agcccgctgg
     3961 cagacgacaa actgtgggtt ctcggtccgg ttccggcact ggcacctaaa cgtggcggtc
     4021 gctggcgctg gcagatattg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
     4081 gtggtacgct ggcgctcatc aatacaatac cggattcccg caaggtgaaa tgggtactgg
     4141 atgttgatcc gattgagggt taaaccgctt acgatgcgag gcggatcgaa aaattcaata
     4201 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     4261 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     4321 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     4381 ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     4441 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
     4501 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
     4561 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     4621 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     4681 caagcaaatt gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg ccagcattga
     4741 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa ttcgcaccgg aactggagct
     4801 acctacagtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atctatatga
     4861 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
     4921 ccgcctgcaa ggttacgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     4981 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
     5041 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     5101 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     5161 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     5221 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     5281 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     5341 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     5401 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     5461 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     5521 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     5581 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     5641 aagtgaccgg taacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     5701 tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     5761 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     5821 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     5881 gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg
     5941 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     6001 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg
     6061 atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg
     6121 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
     6181 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga tacggtacgt gctcagctgg
     6241 cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
     6301 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
     6361 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
     6421 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
     6481 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
     6541 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
     6601 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
     6661 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
     6721 gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
     6781 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
     6841 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gtgcactgtt
     6901 agaaaatact gaactgagtg ctcgtgaaat tgctgaaaag gcgttggata ttgcaggcga
     6961 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
     7021 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
     7081 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
     7141 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
     7201 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
     7261 catcaaagtt gaagcgacca aattcaccga agtgggctac gtcggtaagg aagtggattc
     7321 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
     7381 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
     7441 tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
     7501 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
     7561 cgcagcaccg atgggcgttg aaattatggc tcctccaggc atggaagaga tgaccagcca
     7621 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
     7681 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
     7741 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
     7801 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
     7861 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
     7921 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
     7981 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc tgaccaccag
     8041 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
     8101 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga
     8161 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
     8221 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaactat
     8281 catcattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
     8341 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
     8401 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
     8461 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
     8521 agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactgattac
     8581 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
     8641 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
     8701 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc
     8761 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
     8821 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
     8881 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
     8941 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
     9001 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
     9061 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
     9121 gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
     9181 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
     9241 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
     9301 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
     9361 tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc
     9421 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
     9481 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
     9541 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
     9601 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
     9661 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
     9721 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
     9781 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
     9841 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
     9901 ctttctgaag atccgctgga tattgaatga tagaaaaggc accgcaaggt gccttttttc
     9961 tgcgttacct gttggcctac acagtaaaga aattacgcgg aagatgaagc gtaatcagac
    10021 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
    10081 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
    10141 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
    10201 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
    10261 acctctcctg aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg
    10321 ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg
    10381 tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca
    10441 taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc
    10501 atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga cgaagcacac
    10561 acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta
    10621 caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc
    10681 gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg
    10741 gttgttgatt ttcttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt
    10801 tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac
    10861 cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc
    10921 ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg
    10981 tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca
    11041 tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc
    11101 taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct
    11161 gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc
    11221 tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc cattgacagg
    11281 ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg
    11341 gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc cgcttttcgg
    11401 ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt
    11461 gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag aacaaaaagc
    11521 ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc
    11581 tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta
    11641 gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta gaacacgacc
    11701 caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata
    11761 tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct
    11821 gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg
    11881 cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg
    11941 gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg
    12001 aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc
    12061 ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta
    12121 ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa gtgctggata
    12181 ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca
    12241 ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg
    12301 cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct
    12361 gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca
    12421 ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg
    12481 caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt
    12541 ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta
    12601 ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc
    12661 gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc
    12721 gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg
    12781 atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc
    12841 gcgttgagcg cccggaagtt cgcgaagtga ctgcattggg tgcggcctat cttgctggtc
    12901 tggcggtcgg tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgaacgcg
    12961 agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag
    13021 cggtcaaacg cgcgatggcg tgggaagaac acgacgagta atgtaaatgc cggatgaagc
    13081 gtttatgccg catccggtag tcctgaaacc gtgcgggggc aaccccgcac acatcaataa
    13141 tcccaccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    13201 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca
    13261 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    13321 ttatgctcaa ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg
    13381 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata
    13441 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    13501 cggtgctggc agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga
    13561 agctgattgt cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    13621 acctgcgcaa tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    13681 tagctaaacc acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat
    13741 ttgctattcc ggatggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag
    13801 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc
    13861 gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    13921 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    13981 aagccggtaa agtattgcgc ctgggcgata tggcgcgcag cgataacgtc atcttctctg
    14041 ccaccggtat taccaaaggc gatctgctgg aaggcattag ccgcaaaggc aatatcgcga
    14101 ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc
    14161 actatctgga tcgcaaagac ccggaaatgc aggtgcacat cctctgattg atttgatcga
    14221 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    14281 gttaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    14341 agaactggac cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg
    14401 ccgggcaatt caccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact
    14461 cctatgttaa ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg
    14521 gcaaattaag cccacgattg gcggcactga aaccaggcga tgaagtgcag gtggttagcg
    14581 aagcagctgg cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg
    14641 caaccggtac agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc
    14701 gcttcaaaaa tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc
    14761 cactgatgca agaactggaa aaacgctacg aagggaaact gcgcattcag acggtggtca
    14821 gtcgggaaac ggcagcgggg tcgctcaccg ggcggatacc ggcgttaatt gaaagtgggg
    14881 aactggaaag cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    14941 gcaatccaca gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga
    15001 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    15061 atcgataaac ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc
    15121 cggcatcaaa cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc
    15181 tttttgcata atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt
    15241 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    15301 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    15361 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagagcttt
    15421 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    15481 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    15541 ataatcatct cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc
    15601 agatccttaa tcttgtaggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    15661 agctctttca gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa
    15721 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    15781 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    15841 attacgactt aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga
    15901 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggcattgt
    15961 catttgcatc gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg
    16021 ttaattactt gatttattgt cggctttata cttcacatcc tgggtatctt taccatattt
    16081 attttcgcct tgtgtgccaa tgaacgctcc aagatcgata agcatcatca ccagaatcaa
    16141 cgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg cccagttacc
    16201 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    16261 atgcaagcgc ttaacggtta ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc
    16321 cgcggtctga atatcgagta aatttttacc cgccagtgag aaaagcacca gcatgcctgc
    16381 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    16441 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    16501 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    16561 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    16621 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    16681 tactttcgat ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct
    16741 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    16801 tgcaagtaaa attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa
    16861 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa
    16921 agcgaaagct caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa
    16981 aacacaaagc cagaaaaaac tgcagtcgct gctaacggcg gggaaaaaca aacgttatta
    17041 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    17101 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    17161 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    17221 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    17281 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct
    17341 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    17401 atgtacatcg atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa
    17461 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa
    17521 gacatcggcg ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    17581 tctgacgaac tgatcgcgaa aaaattcgcg gtgctgaaag agcagggcct gactccggtt
    17641 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggca aaactgaaga agtttgcgca
    17701 cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc
    17761 gcttacgaac ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag
    17821 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    17881 gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag
    17941 ccagatatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagta
    18001 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    18061 tccggcactt actttcctta actcttcgcc ttaacgcaaa atctcacact gatgatcctg
    18121 aatttcctcg gctgaagcac ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac
    18181 aaatgagtta tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc
    18241 ctcaggtact tcttctgcca gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    18301 tacccgacgc gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct
    18361 gctgatgttc gccagattgt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat
    18421 atgaatatga aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc
    18481 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    18541 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg
    18601 atacgttggc atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc
    18661 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa
    18721 acattcctca aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta
    18781 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    18841 tttcatcttt ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa
    18901 taaaaaaccc ggtcacgaga aaccaccggg tttgataatt atcctgggga aatcagcgtt
    18961 tgctgatctg atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc
    19021 cgccgaactc ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt
    19081 tcgccacctc agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg
    19141 gcgagtagag atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca
    19201 ctttatcgac caccgacacg gttggctctg cgaggataga ctcactcggc gtgacgattt
    19261 cgaatttatc tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca
    19321 gtacatcgcc aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca
    19381 gaacttcgac gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt
    19441 tgttgtgatg cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc
    19501 ttttcggatt aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga
    19561 tctgcttcgg attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt
    19621 tatccggcag acgtttgatc cactctttat caatccgccc gcgttccgca attgcgtcca
    19681 cgtcataggc cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg
    19741 cttgtttacc tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt
    19801 tccagtgggc gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg
    19861 aaacgttaag aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta
    19921 accctacgcc ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc
    19981 gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgtgggta
    20041 tatgttgagg gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt
    20101 tgtccatgcc ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat
    20161 aaattgcaga attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc
    20221 atcggtttca gggtaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg
    20281 cagtccagcc agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg
    20341 tggtgaacca gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc
    20401 agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa
    20461 ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg
    20521 atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt
    20581 tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca
    20641 acttccggaa ccacaacgaa ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg
    20701 ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca
    20761 cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag
    20821 tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt
    20881 tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg
    20941 atcccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc
    21001 gggaagcgcg cagaaccgag gaacgtaccg ccacggttga tcatgtcaga aacgctgtaa
    21061 cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata aatgcccatt
    21121 acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct
    21181 ggcgcatcac cgccgcttgt caacacaccg attttcttaa tcatgactac ctctgaactt
    21241 tggaatgcaa aataaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga
    21301 atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg
    21361 gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac
    21421 cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataattac atccgatccc
    21481 ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa
    21541 ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc
    21601 gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct
    21661 tgccgttcct catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg
    21721 cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga
    21781 tgccagccgt accaggacaa ccccagcgcc agcagaattg cgccgttcat cataacatca
    21841 gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccaa
    21901 cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg
    21961 acgcctggat ctgtcatcgg tgttggagat atcagatgtt gaatacccgt caaaaacagg
    22021 aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctctgcttta
    22081 ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa
    22141 ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc
    22201 gacccggtat accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc
    22261 gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct
    22321 atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa
    22381 ttgaggataa aaaaaacccc cacatcatgt gggggaagac agggatggtg tctatggcaa
    22441 ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct tcaacgatga
    22501 acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt
    22561 taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga cttttgccat
    22621 ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc gcacagcgtt
    22681 ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc taacattaac
    22741 aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac gctgatgttc
    22801 ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa gttcttcacc
    22861 cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac tgactgccag
    22921 cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa atctttctgt
    22981 cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa
    23041 acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc ggaggtattt
    23101 aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc ctattaaagg
    23161 agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa caggcgcttg
    23221 atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg aagaaaaatg
    23281 gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt atgttgacgg
    23341 cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat gactatctcc
    23401 cgaaaccatt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc
    23461 actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa gttgatgcct
    23521 tagtgctgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg gagttaaccg
    23581 gcactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg gtttcccgtg
    23641 aacatttaag ccaggaagtg ctgggcaaac gcctgacgcc tttcgaccgc gctatcgata
    23701 tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac ccgtggttta
    23761 aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca gcttaaccgc
    23821 gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt
    23881 acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc gtcaggggct
    23941 gatgattgag cagcatgttg aagcggaact ggcgaacgat ccgcccaacg atttaatgtg
    24001 gtggcgacgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc gtttgttatt
    24061 ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa
    24121 ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg gccgcgtgga
    24181 actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt atctgattcg
    24241 tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc cgctattact
    24301 gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct ggagtctggc
    24361 aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa acttacgcca
    24421 gcacccggaa cttgaagcgg ggccacagga attccttgcc gctggtgcca gttttaacca
    24481 gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt ctgatatctc
    24541 tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg
    24601 tagtggtgaa agcaaggaac tggagcgtat tgaaaccgaa gcgcaacgtc tggacagcat
    24661 gatcaacgat ctgttggtga tgtcccgtaa tcagcaaaaa aacgcgctgg ttagcgaaac
    24721 catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg aagccgagca
    24781 gatgggcaag tcgttgacgg ttaacttccc gcctgggccg tggccgctgt acggcaatcc
    24841 gaacgccctg gaaagtgcgc tggaaaatat tgttcgtaat gctctgcgtt attcccatac
    24901 gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg tggacgacga
    24961 tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct atcggaccga
    25021 tgaagcacgc gatcgtgaat ctggcggtac aggattgggg ctggcgattg ttgaaaccgc
    25081 cattcagcag catcgtggct gggtgaaggc agaagacagc ccgctgggcg gtttacggct
    25141 ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg caggataagg
    25201 cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat
    25261 caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc
    25321 cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg gcggcggaga
    25381 gtaaacggtg atattgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct
    25441 gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca gacactttcc
    25501 ctggcgctat cacgctatac aaccacccca ccttacctgt gttttgcatc aactgcgcta
    25561 tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct
    25621 gaattaatgc ctctccccag cggaaaatat cgcccatata aacattactt tccgtcaggc
    25681 cgtcggttga gaggttttca ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa
    25741 attcccgcgc ccagtagaga taatgctcac gaggataatg acacagcgct ctgtctggcc
    25801 cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc gtcagcatca
    25861 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
    25921 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
    25981 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag
    26041 gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag
    26101 gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac tttacgtgac
    26161 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
    26221 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
    26281 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
    26341 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
    26401 aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt attgcccagc
    26461 gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc tcgtaattca
    26521 gggtcaaggt tccccagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
    26581 tgcggttcaa acgaggcaat ccctgcagtg cccagaataa tcatcgtcat gagcggcccg
    26641 gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    26701 atggaagcgt aaagtccgcc attggtcata tccatcgccg ccaccagcgc cagcgttgaa
    26761 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
    26821 gcaaccaccc acgcgacggc aatttttgtt accaccagcg taccggattt acgcaatacc
    26881 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    26941 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    27001 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    27061 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    27121 tgcgtcattc ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
    27181 caataaaagt gatcaaaacc acatcaattg aaacactgtt ttatttttat cggatcattg
    27241 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    27301 gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt ttttcgccgc
    27361 gaaacgcgcc gcagcttcgt cccagttcac cacgttccag aactctttga tgtagtccgg
    27421 gcggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg
    27481 gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt tagcagtaga
    27541 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    27601 agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
    27661 agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct
    27721 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    27781 gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag
    27841 cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    27901 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    27961 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    28021 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
    28081 gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat
    28141 tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
    28201 actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag atgagtaacg
    28261 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    28321 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg
    28381 tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat ttctgggcgt
    28441 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    28501 ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcctgtcg atgggaattg
    28561 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    28621 atttcgatgt gctgattaac accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    28681 cgctgattgg cgtagggatt gtgactcgcg ccgggcagtt gaaagagcgc aaaatgggca
    28741 ttaaagccga agagttcaac ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
    28801 tctctgccgg tatgtctttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
    28861 cactgggcgt cgatccactg tatgtcgctc tgccaagcta tgtcatcatc atgggcggcg
    28921 gcgcgatcat caacctcggt ttctgcttca ttcgtctggc aaaagtgaag gatttgtcgc
    28981 taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcagcac
    29041 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    29101 cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta ttgtgcggcg
    29161 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg tcgccgtccg gtaacggtgt
    29221 tgagcctcgg ttgtgtggtg attattgtcg ccgccaacat cgtcggcatc ggcatggcga
    29281 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    29341 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    29401 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
    29461 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    29521 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
    29581 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    29641 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
    29701 caaacgacac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
    29761 ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
    29821 tcgctccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
    29881 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    29941 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg cagaccatta ccgcgccaga
    30001 caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa aacatcttgc ggataacggt
    30061 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    30121 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
    30181 caaatacgtt gagaagattc gcgttattgc agaaaaccat cccgtccctg acgaatatca
    30241 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    30301 ccacagcgat aggcgatatc agtaacgctg gcctcgctgt ggcgtagtag atgtcgggct
    30361 ttcatcagtc gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg ctgcttaagc
    30421 tgccgatgta gcgtacgcag cgaaagagaa aattgctccg ccacggcatc ccaattcacc
    30481 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    30541 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
    30601 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
    30661 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgggaagg atactgccca
    30721 tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    30781 cgatacagca cattagtcag acacagatta tcggtatgtt catacagatg ccgatcatgg
    30841 tcgcgcacaa aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
    30901 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    30961 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    31021 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    31081 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    31141 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca
    31201 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    31261 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    31321 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    31381 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
    31441 taacaatggg ctgcatagtc agaacggcta tgtcacctgg gatgtggata gcctggaaag
    31501 tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg atagcattgg
    31561 gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc gtgtgggcct
    31621 gcccgttgct tatcgcgata gccgcaccaa tggcctgatg gcgcaggcac aacaacaact
    31681 cggcaaacgc gatatttatc aacgtagcgg catccagttt ctgcccttca atacgcttta
    31741 tcagttacgg gcgctgacgg agcaacaacc tgaacttatt ccacacattg ctcacgctct
    31801 gctaatgccg gattatttta gttatcgcct taccggcaag atgaactggg agtacaccaa
    31861 cgccacaacc acgcaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
    31921 gtggagcggt gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcatagg
    31981 tcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
    32041 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
    32101 tacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcgctggc
    32161 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    32221 gggcttatgg ctgcttcagc gggtgcttca ggaacggcaa atcaacgatc tcccggcgct
    32281 tatcgccgcg acacaggcac ttccggcctg tcgcttcatc atcaatccca atgacgatcg
    32341 ctttattaat cctgaagcga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    32401 accgatcccg gaaagtgatg ctgaactggc gcgctgtatt ttcgacagtc tggcgctgct
    32461 gtatgccgat gtgttgcatg agctggcgca gctgcgcggt gaagatttct cgcaactgca
    32521 tattgtcggc ggcggctgcc agaacacgct gctcaaccag ctatgcgccg atgcctgcgg
    32581 tattcgggtg atcgccgggc ctgttgaagc ctcaacgctc ggcaatatcg gcatccagtt
    32641 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc
    32701 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagat
    32761 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
    32821 gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa
    32881 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
    32941 aacccggaag gttcgctgac cggtgggatt caagctactg gtaattatcc gggcaaagcg
    33001 cgtaatgcca gtgagctacg taccgatctg gaacaggcta tgcggctgat tccggggccg
    33061 aaacggctta atttacatgc catctatctg gaatcagata cgccagtctc gcgcgatcag
    33121 atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    33181 gattttaacc cctcctgctt ttcgcatccg ctaagcgccg atggctttac gctttcccat
    33241 gccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg tcgcgtttcg
    33301 gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat cccggatggt
    33361 atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat
    33421 gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg
    33481 tttggcattg gcgcagagag ctacacggtt ggctccaatg agttttacat ggggtatgcc
    33541 accagccgcc agactgcgct gtgcctggac gccgggcact tccacccgac tgaagtgatt
    33601 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagccgt
    33661 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc
    33721 agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga cttcttcgat
    33781 gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg
    33841 ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc tggaagcggc gggcgattac
    33901 actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa
    33961 atgtattgcc aacgtcacga tacgccagca ggtagcgaat ggctggagag cgtgcgggct
    34021 tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc gcgagcgcct
    34081 tatccggcct acaggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg ccctctccct
    34141 gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca ctgctccgga
    34201 cagtcccctc gcccctacgg ggagagggtt agggtgaagg gaacaagcag caccatgtct
    34261 aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc agtcctggtt
    34321 tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg acgagcgcaa
    34381 cggcggtaac ctgacgctgc gcctggatga cgccgatatc gcgccttata aagacaactt
    34441 ccatgcacaa ccgcgctata tcccgctcag ccaacccatg cctttactgg caaatacacc
    34501 gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagcttgatc ctgcagctaa
    34561 cttaggcgtc gtaaaagtcg acagcgacgg cgcgggctac catattctct gggggttaac
    34621 caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact gcgagcgcat
    34681 taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca acctgatcgc
    34741 cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt gggaaggcag
    34801 caccgagtgt ctggtagtgt tcccggatgg cgttggcatt ttgccgtgga tggtgcccgg
    34861 cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc tggtgttgtg
    34921 gcccttccac ggcgtcttcg gcagcgggtc gacgttggat gaaacattcg gtttaatcga
    34981 caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg gcatgaaaca
    35041 gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta caccactagc
    35101 tagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca tgcaggttaa
    35161 ccccgacgcc cacgaagagt atcagcgtcg gcataacccc atctggccgg aactggaagc
    35221 agtgttgaaa tctcacggtg cgcataacta cgccatttac ctcgacaaag cgcgtaatct
    35281 gctgtttgcc acggtagaga ttgaatctga agaacggtgg aatgcggtag ccagcactga
    35341 tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc cggataacag
    35401 cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca atgtgccaga
    35461 gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat tacatctccc
    35521 ggtaagttat atttccctga tacattgtga gtaaatcaca aaaataatga ataacccatt
    35581 aatgattcat gtggtttatt taaataacca ttatgtgcat tactccgcaa atctgacctt
    35641 tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac ttaatcgctc
    35701 caccattaca attgattaaa atatatttta tggagtccga ttaatggcag ctcttactgc
    35761 aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccgttctga agcagttggc
    35821 gacaatagcg ttacaaaacg gttttatcac cgactcacac cagttcctgc aaaccctgct
    35881 gctgcgcgaa aaaatgcact ctactggatt tggttccggt gtcgccgtgc cgcacggtaa
    35941 aagcgcctgc gttaaacaac cgttcgtatt attcgcccgc aaagcgcagg ctattgactg
    36001 gaaagccagc gatggcgaag acgtcaattg ctggatctgc ctcggcgtgc cgcaaagcgg
    36061 cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc aaaattattc acaaggaatt
    36121 tattcatcaa ctgcaacagg gcgataccga ccaggtgctt gccttgttaa atcaaaccct
    36181 cagctcataa ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc accaactgcc
    36241 ccgccgggat cgctcacacc tacatggtgg cggaagccct ggaacagaaa gcccgttctc
    36301 tcggtcatac cattaaagtt gaaactcaag gatcaagcgg cgtcgaaaac cgcttatcca
    36361 gcgaagagat tgccgctgcc gattacgtca ttctcgctac cgggcgtggc ctgagcggtg
    36421 acgatcgcgc gcggtttgcc gggaagaagg tttatgagat tgccatctcc caggcgctga
    36481 aaaatatcga tcagattttc agtgaattac caacaaactc gcatcttttt gctgcagata
    36541 gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc cacctgatgg
    36601 cgggcgtctc tgcggcgctg ccgtttgtca tcggcggcgg tattctggtg gcgcttgcca
    36661 acatgctggt gcagttcggt ttgccatata ccgatatgtc gaaaggtgcg ccgtcgttta
    36721 cctgggtagt tgaatccatc ggttacctcg gcttcacctt tatgatcccc atcatgggcg
    36781 cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt ctggtttgct
    36841 atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc ctcggcgcgg
    36901 tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgcaaagtg cgccttggca
    36961 aagcattaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg ctggttttcg
    37021 gcgtgctcac ttactacgtt atcgggccgg tgatgtccga cctcatgggc gggctgctcc
    37081 acttcctgaa caccattcct ccatcaatga agtttgcggc agcgtttctg gtcggtgcga
    37141 tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc tgcttctcac
    37201 tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg ttgatgccgc
    37261 ccgtcgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga caggaaaaag
    37321 aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg gctattcctt
    37381 acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggc ggcattaccg
    37441 gcgtgctggt gatcgctttc ggaatcaaac gtctggcgcc tggtttgggt atctttgacc
    37501 cgctgatcgg cctgatgtcg ccggtaggtt cgttttatct ggtgctggcg attggcctcg
    37561 cgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc aaagcgaaag
    37621 ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg cgaagccagc
    37681 gccgtcagcg gcgatgaaca ggaagttcgc gacatcctga taaacacgct ggaaccttgc
    37741 gttaatgaaa tcacctttga tggtctgggc agctttgttg cccgtaaggg gaataaaggt
    37801 ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac ccacatcgac
    37861 gagagcggtt ttctgcgttt taccaccatt ggcggctggt ggaatcagtc gatgctcaac
    37921 caccgggtaa ccatacgcac acacaaggga gtgaaaatcc ctggtgtgat tggttccgtc
    37981 gcgcctcatg cgttaacgga aaagcaaaag caacaaccgc tgtcatttga tgagatgttc
    38041 attgatattg gcgcgaacag tcgcgatgag gtggaaaaac gcggtgtggc gatgggcgat
    38101 tttattagcc cggaagccaa ttttgcctgc tggggcgaag ataaagtggt cggcaaggcg
    38161 ctggataacc gcatcggctg cgcaatgatg gctgaactat tgcagacggt gaataatccc
    38221 gaaattacgc tgtatggcgt cggcagcgtg gaagaagaag ttgggctacg cggggcacaa
    38281 acctcggcgg aacacattaa accggatgta gtgatcgtgc tggataccgc tgtcgcaggc
    38341 gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg gccggggctg
    38401 atgctgtttg acaagcgcta cttccccaac cagaaactgg tagcagcgtt aaaaagctgt
    38461 gccgcacata acgatttacc gctgcaattt tccaccatga aaaccggtgc gacggatggc
    38521 gggcgctaca acgtgatggg cggcgggcgt ccggttgttg cgctgtgcct gccaacccgc
    38581 tatctgcacg ctaacagcgg tatgatttca aaagccgatt acgatgctct gctcacgttg
    38641 atacgggatt ttctgaccac cttaaccgcg gagaaagtca acgcgtttag ccagttccgt
    38701 caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg gagcaacagc
    38761 cgcgcacgcc tggtgagctg gcgcaacaga ctggcgtttc aggcaggacc atcctgcgtg
    38821 atattgacta tctcaacttt acccttaacg gcaaagcccg catttccgcc agtggtagtg
    38881 cgggctatca gctggaaatc ttcgagcgcc gcagcttttt tcagttgctg caaaagcacg
    38941 ataacgacga tcggctgctg gcactgttat tactgaatac ctttactccc cgtgcgcaac
    39001 tcgcctcggc gcttaatttg ccagaaacgt gggtcgcaga gcgtctgccc cggttaaaac
    39061 agcgttatga acgcacttgt tgcctggcca gccgccctgg tttgggccat ttcattgatg
    39121 agacagaaga gaaacgcgtt atcttgctgg cgaacttgct gcgcaaagat ccgtttttaa
    39181 ttccgctggc gggcgtaaca cgagacaacc ttcagcattt atccacggcc tgcgacaacc
    39241 aacaccgctg gccgctcatg cagggtgatt atctctccag cctgattctg gcgatttacg
    39301 ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc cggtgacgag ataaaacaaa
    39361 tcgttgaaca tagcggtctg tttcttggtg ataacgctgt aagaaccctg acaggtttga
    39421 tagagaaaca gcatcagcaa gcgcagataa tttctgccga taatgtgcag cggttgctgc
    39481 aaagggtgcc gggcatcgcg tcattgaata ttattgatac gcagctggtt gagaacatta
    39541 ccgggcattt attacgttgc cttgccgcac cggtgtggat tgctgagcac cgccaaagca
    39601 gcatgaataa cctgaaagcc gcctggcctg cggcctttga tatgagtctg cactttatta
    39661 cgctactgcg tgaacagctc gatattcccc ttttcgacag cgatctgatc ggtttgtatt
    39721 ttgcctgtgc gctggaacgg catcaaaacg aacgccagcc gatcattttg ctctcggacc
    39781 agaacgcgat tgccactatt aatcagctcg ccattgagcg cgatgtttta cattgtcggg
    39841 tgattattgc ccgtagctta agcgaacttg ttgccattcg cgaagagatt gagccgttat
    39901 tgatcattaa caacagccat tatttactgg atgacgcggt aaataattac atcaccgtaa
    39961 aaaatatcat tacggctgcc ggtatcgaac aaataaaaca ttttctggcg acggcattta
    40021 ttcgccaaca gccggagcgt tttttctctg cccccggaag ttttcattat tcgaatgtac
    40081 gcggtgaaag ctggcaacat attacccggc aaatttgtgc gcaattagtg gcacaacacc
    40141 atattaccgc cgatgaagca caacgcatca tcgcccgcga aggcgaaggt gaaaacctga
    40201 ttgttaatcg cctcgccatc ccacattgct ggagcgaaca ggagcgacgt tttcgtggat
    40261 tttttattac cctcgcccaa ccagttgagg tgaataacga agtcattaac catgtcttga
    40321 tcgcctgcgc cgccgccgat gcgcgtcatg agctaaaaat atttagctat ctggcaagcg
    40381 tattgtgtca gcatccggca gaggttattg ccgggttaac aggatatgag gcatttatgg
    40441 agttacttca caaggggtga actgtgtcgg atgcgccgca tccgacactg catttatcat
    40501 caaaaacgtt taacgcaggc tattatagtc atcaaccatt tcgttgtact cgcgtaatgc
    40561 tctcgggaaa gagtcttcga ccatccagct tgagtttgcg tcctgaagtt gctctttatc
    40621 ccagtcagag tacgggactt tatctctgat gcggcgaacg tattttttag cctcgagttg
    40681 atactggcct gccgaattaa taaacgagaa attcatgccg cctttatccg cttctttggc
    40741 ctgcgccact agcgtttcca gttcagacac tttcttcatt gccgcatcaa catcaaactt
    40801 ctcttgcgat atcaggttat taatttgttt cgctgaaatc atgactgcca gagagtagta
    40861 gtggaatgtt ttcccttctc tttcttcaat atttttcagt tccgcaagct ggcgcttatc
    40921 gttaatttcc tgaatcgccg catgataaga ttctgctaca ggttcaaagg cttccagatt
    40981 ttttaagaat gtctggtgca gcgttttccc tttcgcaaac gcatcgtctt tataattttc
    41041 ctgggtgtaa tatttatcca tttcgttaat ggtattaccc aacgccacgg cagcatcgat
    41101 ataactcacc gccacgccat caattggttg cagcgcaggc gttaacgcca cagcactttt
    41161 tacgcctttt tcacactcag cgaggttgga ttcactaatg ccgtagatgc cataaacagt
    41221 acgctcttca ccggtaggcc cctgtttaaa atctttcagc cagtcagcat aacgcgccag
    41281 actgctctgt acagggatct gtaacttgtt atagcacttg atatagacat ccattttttc
    41341 gtcgaaaacc gcttgttcat cgaccaccgg ttgcgcaggt gtttcaggtt tagcttctgc
    41401 ttttgcaacg ggagcttcag gagctggtgc ggcaggttga ctattggcag gcgggggcgt
    41461 ttctgtttcg gcttttttat catcacaacc cgtcagaccg agggccatga tggcgactat
    41521 aatagcggaa gataataaat tcctt
//
LOCUS       DAZGCS010000036        41198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGCS010000036 DAZGCS010000000
VERSION     DAZGCS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.036"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..81
                     /locus_tag="ACHK01_001959"
     tRNA            5..81
                     /locus_tag="ACHK01_001959"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Met,seq:cat)
     gene            130..204
                     /locus_tag="ACHK01_001960"
     tRNA            130..204
                     /locus_tag="ACHK01_001960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Gln,seq:ctg)
     gene            242..316
                     /locus_tag="ACHK01_001961"
     tRNA            242..316
                     /locus_tag="ACHK01_001961"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Gln,seq:ctg)
     gene            complement(470..1645)
                     /gene="ubiF"
                     /locus_tag="ACHK01_001962"
     CDS             complement(470..1645)
                     /gene="ubiF"
                     /locus_tag="ACHK01_001962"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO8096918.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1791..3215
                     /gene="miaB"
                     /locus_tag="ACHK01_001963"
     CDS             1791..3215
                     /gene="miaB"
                     /locus_tag="ACHK01_001963"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO8096919.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3368..4408
                     /gene="ybeZ"
                     /locus_tag="ACHK01_001964"
     CDS             3368..4408
                     /gene="ybeZ"
                     /locus_tag="ACHK01_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO8096920.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4405..4872
                     /gene="ybeY"
                     /locus_tag="ACHK01_001965"
     CDS             4405..4872
                     /gene="ybeY"
                     /locus_tag="ACHK01_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO8096921.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4962..5840
                     /gene="corC"
                     /locus_tag="ACHK01_001966"
     CDS             4962..5840
                     /gene="corC"
                     /locus_tag="ACHK01_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO8096922.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5865..7403
                     /gene="lnt"
                     /locus_tag="ACHK01_001967"
     CDS             5865..7403
                     /gene="lnt"
                     /locus_tag="ACHK01_001967"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO8096923.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYKSADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7481..9358)
                     /locus_tag="ACHK01_001968"
     CDS             complement(7481..9358)
                     /locus_tag="ACHK01_001968"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO8096924.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9813..10721
                     /gene="gltI"
                     /locus_tag="ACHK01_001969"
     CDS             9813..10721
                     /gene="gltI"
                     /locus_tag="ACHK01_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO8096925.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10891..11631
                     /gene="gltJ"
                     /locus_tag="ACHK01_001970"
     CDS             10891..11631
                     /gene="gltJ"
                     /locus_tag="ACHK01_001970"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO8096926.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11631..12305
                     /gene="gltK"
                     /locus_tag="ACHK01_001971"
     CDS             11631..12305
                     /gene="gltK"
                     /locus_tag="ACHK01_001971"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO8096927.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12305..13030
                     /gene="gltL"
                     /locus_tag="ACHK01_001972"
     CDS             12305..13030
                     /gene="gltL"
                     /locus_tag="ACHK01_001972"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO8096928.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13148..14083
                     /gene="rihA"
                     /locus_tag="ACHK01_001973"
     CDS             13148..14083
                     /gene="rihA"
                     /locus_tag="ACHK01_001973"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO8096929.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            14167..15837
                     /gene="hscC"
                     /locus_tag="ACHK01_001974"
     CDS             14167..15837
                     /gene="hscC"
                     /locus_tag="ACHK01_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO8096930.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(15897..17348)
                     /gene="djlC"
                     /locus_tag="ACHK01_001975"
     CDS             complement(15897..17348)
                     /gene="djlC"
                     /locus_tag="ACHK01_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO8096931.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(17345..18052)
                     /gene="ybeU"
                     /locus_tag="ACHK01_001976"
     CDS             complement(17345..18052)
                     /gene="ybeU"
                     /locus_tag="ACHK01_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8096932.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEDSLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            18153..18707
                     /gene="ybeT"
                     /locus_tag="ACHK01_001977"
     CDS             18153..18707
                     /gene="ybeT"
                     /locus_tag="ACHK01_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8096933.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18717..20144)
                     /gene="djlB"
                     /locus_tag="ACHK01_001978"
     CDS             complement(18717..20144)
                     /gene="djlB"
                     /locus_tag="ACHK01_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO8096934.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(20141..20848)
                     /gene="ybeR"
                     /locus_tag="ACHK01_001979"
     CDS             complement(20141..20848)
                     /gene="ybeR"
                     /locus_tag="ACHK01_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8096935.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            21012..21989
                     /gene="ybeQ"
                     /locus_tag="ACHK01_001980"
     CDS             21012..21989
                     /gene="ybeQ"
                     /locus_tag="ACHK01_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO8096936.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(22059..22541)
                     /gene="ybeL"
                     /locus_tag="ACHK01_001981"
     CDS             complement(22059..22541)
                     /gene="ybeL"
                     /locus_tag="ACHK01_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO8096937.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFIRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22776..25358
                     /gene="leuS"
                     /locus_tag="ACHK01_001982"
     CDS             22776..25358
                     /gene="leuS"
                     /locus_tag="ACHK01_001982"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO8096938.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25373..25954
                     /gene="lptE"
                     /locus_tag="ACHK01_001983"
     CDS             25373..25954
                     /gene="lptE"
                     /locus_tag="ACHK01_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO8096939.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25954..26985
                     /gene="holA"
                     /locus_tag="ACHK01_001984"
     CDS             25954..26985
                     /gene="holA"
                     /locus_tag="ACHK01_001984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO8096940.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHN
                     PLADVFIDG"
     gene            26987..27628
                     /gene="nadD"
                     /locus_tag="ACHK01_001985"
     CDS             26987..27628
                     /gene="nadD"
                     /locus_tag="ACHK01_001985"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO8096941.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27652..28263
                     /gene="cobC"
                     /locus_tag="ACHK01_001986"
     CDS             27652..28263
                     /gene="cobC"
                     /locus_tag="ACHK01_001986"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO8096942.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            28523..28840
                     /gene="rsfS"
                     /locus_tag="ACHK01_001987"
     CDS             28523..28840
                     /gene="rsfS"
                     /locus_tag="ACHK01_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO8096943.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28844..29311
                     /gene="rlmH"
                     /locus_tag="ACHK01_001988"
     CDS             28844..29311
                     /gene="rlmH"
                     /locus_tag="ACHK01_001988"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO8096944.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29342..31243
                     /gene="mrdA"
                     /locus_tag="ACHK01_001989"
     CDS             29342..31243
                     /gene="mrdA"
                     /locus_tag="ACHK01_001989"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO8096945.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVV
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31246..32358
                     /gene="mrdB"
                     /locus_tag="ACHK01_001990"
                     /gene_synonym="rodA"
     CDS             31246..32358
                     /gene="mrdB"
                     /locus_tag="ACHK01_001990"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO8096946.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32369..33457
                     /gene="rlpA"
                     /locus_tag="ACHK01_001991"
     CDS             32369..33457
                     /gene="rlpA"
                     /locus_tag="ACHK01_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO8096947.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33597..34808
                     /gene="dacA"
                     /locus_tag="ACHK01_001992"
     CDS             33597..34808
                     /gene="dacA"
                     /locus_tag="ACHK01_001992"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO8096948.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34919..35182
                     /gene="ybeD"
                     /locus_tag="ACHK01_001993"
     CDS             34919..35182
                     /gene="ybeD"
                     /locus_tag="ACHK01_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO8096949.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35283..35924
                     /gene="lipB"
                     /locus_tag="ACHK01_001994"
     CDS             35283..35924
                     /gene="lipB"
                     /locus_tag="ACHK01_001994"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO8096950.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIVPRLLENILALLNNPDFEYITA"
     gene            36183..37136
                     /gene="ybeF"
                     /locus_tag="ACHK01_001995"
     CDS             36183..37136
                     /gene="ybeF"
                     /locus_tag="ACHK01_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO8096951.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINLTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37345..38310
                     /gene="lipA"
                     /locus_tag="ACHK01_001996"
     CDS             37345..38310
                     /gene="lipA"
                     /locus_tag="ACHK01_001996"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO8096952.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(38411..38614)
                     /gene="tatE"
                     /locus_tag="ACHK01_001997"
     CDS             complement(38411..38614)
                     /gene="tatE"
                     /locus_tag="ACHK01_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO8096953.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(38743..39531)
                     /locus_tag="ACHK01_001998"
     CDS             complement(38743..39531)
                     /locus_tag="ACHK01_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO8096954.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGELLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            39624..40007
                     /gene="crcB"
                     /locus_tag="ACHK01_001999"
     CDS             39624..40007
                     /gene="crcB"
                     /locus_tag="ACHK01_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO8096955.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(40062..40271)
                     /gene="cspE"
                     /locus_tag="ACHK01_002000"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(40062..40271)
                     /gene="cspE"
                     /locus_tag="ACHK01_002000"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8096956.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(40446..41006)
                     /gene="pagP"
                     /locus_tag="ACHK01_002001"
     CDS             complement(40446..41006)
                     /gene="pagP"
                     /locus_tag="ACHK01_002001"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO8096957.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        10371 a        10484 c        10257 g        10086 t
ORIGIN      
        1 atctggctac gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt
       61 cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa
      121 ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
      181 gttcgaatcc tcgtacccca gccaatttat tcaagacgct tacatcgtaa gtgcgcccag
      241 ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat
      301 cctcgtaccc cagccacatt aaaaaagctc gcttcggcga gctttttgct tttctgcgtt
      361 cattcaatgt cgaatgcgat gttgacacgt cttatccttc aatgtcggat gcgacgctgc
      421 cgcgtcttat ccgacctacg gttggcacgc atccggcaat gttgtaaggc tacaacccta
      481 acgcatattt cagcgcctga cgtttcaaca cgccagcacg ctctgccgcc attaacccga
      541 gattacgcac aaaacgcagc ggcggcagat tattactgaa tccggcataa aacagatcca
      601 taccactttg cataatgaag ttatccgcca tgcgccgcat ctggtaacgc ttgaggacag
      661 gataactggc ccacgcttca ccgtagctgc gggcattcac cagaacatca atcagggcat
      721 cgacatcacg ataaccaaga ttcaccccct gccccgccag cggatggatg gtatgcgcgg
      781 catcgcccac cagcgcaagc cccggctgca catactgcaa cgcatgtcgg cgcgtcagcg
      841 gaaacgcacc agcggcaagc ggtgtaacgt aacccagacg cgacgggaaa tgcttcgcga
      901 tttccgcctg gagttgcgcc atattcatat tctgcaactg gcgaatacgt accggagagt
      961 cataccacac cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg
     1021 gggtaaattg ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg ctaatcaaca
     1081 tacacgactg cgcatactgc caggcatgaa cgccaattcc cgccatctgc cgcacctgcg
     1141 aatttgcgcc gtcagcacca atcaccagct tcgcgcgaat cacttcaccg cccttcagtt
     1201 ccagctcctg aagatcatta tctcgatgta atgcaatcag cgagcctggc acacgtaacg
     1261 ttactttcgg atgcgcttcc agcgcctgcc acaatgcctg ttgcaggacg gtgttttcca
     1321 ccatatagcc aagcagcggc agcttaagtt cagcggcgtc aaacatcaca tgcgccgttt
     1381 cccactccca cgtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc
     1441 agacccctaa ccctttaagc aatgataccg atgcggcgct gatcgccgag atccgcacgt
     1501 ccggttggct atcagcgaca aacggtgcag gttctgcgtg ctcgatcacc gttaccgaaa
     1561 atccgtgctg tgccagcccc agcgccagtg cgccgccgac cattcctccg ccgacaatgg
     1621 caatttccgt tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt
     1681 acaggatttt tacccccaag ggctgataac gctcatactg gtcacaacgg cagcaaagca
     1741 ttacactatg cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa
     1801 aactccatat taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg
     1861 atctgctgga tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc
     1921 tgctgctgaa cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg
     1981 gtcgctggaa actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggtggctgcg
     2041 tggcatcgca agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg
     2101 ggccgcaaac gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc
     2161 cggttgtaga tatcagcttc ccggaaatcg agaagtttga ccgtctgccg gaaccgcgcg
     2221 ccgaagggcc gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact
     2281 gcgtggtgcc ttacacccgt ggtgaagagg taagccgtcc gtccgacgat attctgtttg
     2341 agatcgccca gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt cagaatgtga
     2401 atgcctggcg tggtgagaac tacgacggca ccaccggatc gtttgccgat ctgttgcgtc
     2461 tggttgctgc gatcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat
     2521 tcaccgacga tatcatcgaa gtgtatcgcg acacgccgga gctggtgagc ttcctgcatc
     2581 tgccggtaca gagcggttcc gatcgcattc tgaacctgat gggccgtact catacggcac
     2641 tggagtacaa agcgatcatc cgtaaactgc gtgcggcgcg tccggatatt cagatcagtt
     2701 ctgacttcat tgttggcttc cctggcgaaa ccaccgaaga cttcgagaaa acgatgaagc
     2761 tgattgccga cgtcaatttc gacatgagct acagctttat cttctctgca cgtccaggaa
     2821 cgcctgccgc tgatatggtt gatgatgttc cggaagaaga gaagaagcag cgtctgtata
     2881 ttctgcaaga acgtatcaat cagcaagcga tggcatggag ccgtcggatg ctcggcacca
     2941 cccagcgtat tctggtagaa ggtacatcgc gtaagagcat catggagctt tccgggcgta
     3001 ccgaaaataa ccgtgtggtc aacttcgagg gcacaccgga tatgatcggt aaattcgtcg
     3061 atgtagaaat taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgtactgaag
     3121 atgaaatggg tctgcgcgtg gcagaaacac ccgaatcggt gattgcccgt actcgcaaag
     3181 aaaatgacct tggcgtgggt tattatcagc cgtaattctc aggcccgccg ttccggtggg
     3241 ccttgttttt tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg
     3301 cgtgaataat ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag
     3361 gaacggtttg aacatagaca ctcgcgaaat caccctggag ccagcagaca atgcgcgtct
     3421 gttgagcctg tgcggcccgt ttgatgacaa catcaagcag ctcgaacgcc gtctcggcat
     3481 cgagatcaat cgccgcgaca accactttaa actgaccggc cgtccgattt gcgtcaccgc
     3541 tgcggcagac attctgcgta gcctgtatgt cgatacagcc ccgatgcgcg gtcagattca
     3601 ggatatcgaa ccggaacaga tccaccttgc gattaaagaa gcgcgagtac tggagcaaag
     3661 tgcggagagc gtgccggagt acggcaaagc ggtcaatatc aaaaccaaac gcggcgtaat
     3721 taagccgcgc acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac
     3781 cttcggcgtt ggcccggcgg gtacgggtaa aacctacctg gcagtggctg cggcagttga
     3841 tgccctggag cgtcaggaga ttcgccgtat tctgctgact cgtccggcgg tcgaagccgg
     3901 tgagaaactg ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc
     3961 gctgtacgac gcgctgtttg aaatgctggg ctttgagaaa gttgagaaac tgattgagcg
     4021 caacgttatt gaagtcgcgc cgctggccta tatgcgtggt cgtacgctga acgacgcgtt
     4081 tatcattctc gatgagagcc agaacaccac catcgaacag atgaagatgt tcctgacccg
     4141 tatcggtttt aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg
     4201 taatactaaa tcaggcttac gtcacgccat cgaagtgctg gccgatgtcg aagagatcag
     4261 ctttaacttc ttccacagcg aagacgtggt tcgtcacccg gtggtggcgc gtatcgttaa
     4321 cgcctatgaa gcctgggaag aagccgaaca aaaacgaaaa gcggcgctgg cggcagaacg
     4381 caagcgcgaa gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt
     4441 gaagataatt ctgggttacc ggaagagagc cagtttcaaa catggctgaa tgcggtgatc
     4501 ccgcagtttc aggaagaatc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac
     4561 agtctgaatc tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt
     4621 gaagtgccgc ctggcatgga aatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg
     4681 gttgagaagg aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg
     4741 gtgcacggca gtctgcattt gttaggttac gatcacatcg aagatgacga agcagaagaa
     4801 atggaagccc tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc
     4861 gagaaagaat aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg
     4921 acggcgtttt gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac
     4981 acagtagtga cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt
     5041 tccacggtga accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga
     5101 acgaccttat cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc
     5161 aacgcgttcg cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga
     5221 cgctggacga atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta
     5281 gcgaagacaa agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc
     5341 gcagcgatgc tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg
     5401 aaagtaagcg cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga
     5461 tcgttattga cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac
     5521 taattgttgg tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc
     5581 tgagtcgtca tacctggacc gtgcgcgcac tggcttccat tgaagacttc aacgaagcgt
     5641 tcggcaccca ctttagcgat gaagaggttg acactatcgg tggtctggtg atgcaggcat
     5701 ttgggcatct tccggcgcgt ggcgaaacta tcgacatcga cggttaccag ttcaaagtgg
     5761 cgatggccga cagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc
     5821 agccgaagct ggatgaataa aaccgaaact ggatagataa ctacatggct tttgcctcat
     5881 taattgaacg ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc
     5941 tggccttctc tccttacgat gtctggcctg cggcgattat ttcgctgatg gggcttcagg
     6001 cgttgacctt taaccgccgt ccactccagt ctgccgctat tggcttttgc tggggatttg
     6061 gcctctttgg cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcgggatgc
     6121 ctggcccggt taacatcttc ctggtggtgc tgctggcggc gtatttgtcg ctgtataccg
     6181 gactgtttgc tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga
     6241 ttgccgcccc ttctctctgg caagtgaccg agtttctgcg cggttgggta ctgacaggct
     6301 tcccgtggtt acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccga
     6361 taatgggcgt ggaagccatt aacttcctgc tgatgatggt tagtggcctg ctggcactgg
     6421 cgttggtcaa acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gccctaccct
     6481 tcccgctgcg ttacatccaa tggtttaccc cacaaccgga gaaaaccatt caggtttcga
     6541 tggttcaggg cgatattccg caatcactga aatgggatgg agatcagctt cttaatacgc
     6601 tgaagattta ctacaacgca acggctccgc tgatgggcaa atcatcgttg attatctggc
     6661 cggagtcggc gataaccgat ctggaaatta atcagcaacc gttcctcaaa gcactggacg
     6721 gtgagttgcg cgataaaggt agctcgctgg taaccgggat tgtcgacgcg cgtctcaata
     6781 agcagaaccg ctacgatacc tacaacacca tcatcacgct gggtaaaggt gcaccgtaca
     6841 gctacaaatc agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc
     6901 cgctggagtc gattctgcgt ccgttagcac cgttctttga tctgccgatg tcgtcgttca
     6961 gccgtgggcc atatatccag ccgccgctgt cggcaaatgg tattgagctt actgcggcta
     7021 tttgctacga gatcattctc ggcgagcaag tgcgcgataa cttccgcccg gataccgact
     7081 atctgctgac tatctccaac gatgcgtggt ttggtaagtc tattggtcca tggcagcact
     7141 tccagatggc gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca
     7201 acggcattac ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttca
     7261 cccgcgaggt gttgaccact aatgtgacac cgaccaccgg actcacaccg tatgcacgta
     7321 ccggcaactg gccgctgtgg gtgctgacgg cgctgtttgg ttttgccgct gtgctgatga
     7381 gtctgcgagc acgtaaacgt tgatgtgcct gatgtatcct gcatgagcaa aacgtacttt
     7441 ctcattaagc tttggggcat ggttatcatg cccctttttc ttacggttca gggatacaag
     7501 ccacattgcc atattgggga tgacgaataa ttgcctgcgg atcaagcgag cgcgtcctgt
     7561 ccatttggat caacccgttc ttcgccatga caaatatttt tctaccatcc gctgaaaaac
     7621 gaaggtgatt aacgtagctg gcaggataaa cgccaataat ctgcccggca ttaatattga
     7681 gcaggatgac ataaccttgc agtccatatg acaccaacgc taaatattga ccgtccgggc
     7741 taataccctc aacggaataa acgctcctgc tacaccattg cgtcataatg ctggtttgtt
     7801 gatcccctat gcttatacgt gaaatcccct gactatcggc aaaaataacc tcgccgtttt
     7861 caggtcgaaa acgcatcgcc gatatcgttc cacgcgcatc acctgcggga aagtcttctt
     7921 tctgatgcgt gatggtattg atccgcacga tgttacctct gccttctccc ggaaccagta
     7981 atgttctttc atccggggaa agtgccataa cccaggcgcc tggtttaccg ctcgtccccg
     8041 tcccgtcgta tttctccagc gggatatccg ccacatgctg ataagccacc agatcaatgg
     8101 ctgacacact attactggtg cgattcatca cccaggcttt tgtgccatca cgcgagagga
     8161 taagactgtc aggatgtgca ccggtggtaa tcgactgaat aatctcccct gtcgccacgt
     8221 taatgaccga taacgcattc gcttcaaatg aagtcgcata aatcaacttt tcatccgggc
     8281 ttaatgccag cgatctgacg ccctgatctt tacagccacc acaatgtttg actgactttt
     8341 ctttcgctat gggcgctgta aaggtatgca ggattttctt ttgtccgagg tcgaaaacaa
     8401 caatgctgtt ggtgtcttca attgcggcat acaacgtatt accatcccga ctgatcgcca
     8461 tatcatggat atcggcaatc tcagcgagca aatcaccact ggcctgttca cgacttaccg
     8521 gagcggataa tgtttccctg agggcgttgc gttcctccgc taactgctgc tttttttgcc
     8581 gtcgctcttg ttcaatctcc tgtggataaa acgcttcacg caggctttgc ctgacttgta
     8641 aaactgactc atccatctgc tgctgcgcaa gccagatagc cccggtcaga agaagactgc
     8701 ccgcaacaat aatcccgcct tccgtaacga gtcggttttg cccagataaa cgcgcgctca
     8761 gccaacccaa cgcgccaccc gcaatcaggc cgccaatgtg gcaagcgtta tctattcctg
     8821 tttgccgggc accgtacaac agtgtcagcg ccaccatacc caacagagga aatactcgcc
     8881 gctggttttt atggtatgtg cccgtacctg aagcaaactg tgttgctatc gctgcaccag
     8941 ctattcccag aatcgccccg gatgccccaa cgctgatgac tatctgctca ctttcgcgta
     9001 acgcataaca ggcgctgatg agcccaccaa agacgccgga gaataaccag gtaatcagga
     9061 gtcgccattt ccccagaatc tgttccgcca cgcctcccac gaccaggaat gcaaacatat
     9121 tcatcagcag atgagaaaca ctactgtgca gaaagatact gctcaataag cgccagggtt
     9181 cacccgacaa agtaagaggc gcaacgtttg cgccgagatg gataagtgcg gattgatcga
     9241 cctcttcata actcatccat tgtgcgagat aacaaaatat cccaatacag agtgcaaaaa
     9301 ggataagggt tattgcgggt aattgaacat ttaacggctt aaccgaagat gcagacataa
     9361 ggtgtttctt ccttgaaaag acaggtaaaa agcatttttc ccgtgtgcag ttatcatccc
     9421 ggcaaacgtc ccatgccgaa aagataattc tgtccgaaat atgacttttt cagcctaaaa
     9481 ttacaatcgt aattaattcg cccctaagta ttttttggca tgtaagttgc tttatttact
     9541 gtgtaagcga tgaaatcagc cgcgacgcaa ccaggttgca ccaccacaaa tcagcggtag
     9601 caccaaaaag gtgcggttta agaattttgc ttcggaatgg tgcggcgcat ctcgcaaaaa
     9661 caaacaataa aacagcaata tctttacatt tagccaaact aaatgttacc taacaggcac
     9721 aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg ggtatccatg
     9781 cgttcttaac gcagaagata aaggagttgg atatgcaatt acgtaaacct gccacagcaa
     9841 tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggtagcgg
     9901 gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcat cgtgaatctt
     9961 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg caggattact
    10021 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac
    10081 tgattccgat tacctcacaa aaccgtattc cgctgctgca aaacggcact tttgattttg
    10141 aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
    10201 ttttcgtggt cggtacgcgc ctgctgacca aaaagggcgg cgatatcaaa gattttgccg
    10261 acctgaaaga caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
    10321 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    10381 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgat gccctgctgg
    10441 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc aagccgcagt
    10501 ctcaggaggc ctacggttgt atgttgcgta aagatgatcc gcagttcaaa aagctgatgg
    10561 atgacaccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt
    10621 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    10681 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    10741 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc
    10801 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    10861 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    10921 ttacaacaag ccccgttcgg caacaccacc tatcttggtt ggatctggag tggttttcag
    10981 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    11041 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    11101 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag
    11161 ctgttgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt
    11221 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    11281 cgcgccgcta ttcagtcgct cccgcgtggg cagaagaatg ccgcgctggc gatgggcctg
    11341 acgctaccgc aggcttaccg ctatgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    11401 ccgatgacct ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gactatcggt
    11461 ctggtggata tggcagcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag
    11521 tcgtttaccg ccattacgct ggcgtatgta ttgattaacg cggttatcat gctggtgatg
    11581 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    11641 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcacgc
    11701 tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggcaccatg ctggcggtga
    11761 tgcgcttgtc cagctttgcg cccgtagcgt ggtttgccaa agcctacgtt aacgtattcc
    11821 gctcgattcc tttagttatg gttttgctgt ggttttacct gatcgtgccg ggttttctgc
    11881 aaaacgtgct gggattatcg ccaaaaaatg atatccgttt aatttcggcg atggtggcgt
    11941 tttcgatgtt tgaagcggcc tactattcag agattatccg tgctggtatt caaagtattt
    12001 ctcgtggtca gtcgagcgcc gcgctggcgt tgggaatgac tcactggcag tcgatgaaac
    12061 tgattattct gccgcaggcg ttccgcgcga tggtgccgct tctgctcact cagggcatcg
    12121 tactgttcca ggatacctca ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    12181 cctcaaccat tggtgagcgt gatggtacgc aggttgagat gatcctgttt gccggatttg
    12241 tttatttcgt tattagtctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    12301 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    12361 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggctccgg
    12421 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac
    12481 cgtcgatggt atcgtggtta atgacaagaa aaccgatctg gcaaagctgc gttcccgcgt
    12541 cgggatggtg ttccagcatt ttgagttgtt ccctcatctg tcgattatcg aaaacctgac
    12601 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccg gctcgtgaaa aagcattgaa
    12661 attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg
    12721 cggtcagcag cagcgtgtgg cgatcgctcg cgcattgtgt atggatccta ttgcgatgct
    12781 gttcgacgaa ccgacgtcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    12841 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    12901 ccgcaaagtg gcgaatcggg tgatctttat ggatgagggt aaaattgtcg aagactcgcc
    12961 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    13021 cctgcattaa tcactctggc gcgcataccc aagtgcgcgc catctcacgc tttatcgttt
    13081 cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg
    13141 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg acgctatcgc
    13201 aatagttctc gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    13261 aaaccagaca ccagaaaaaa ccttacgcaa tgttcttcgt atgctgacct tgctcaatcg
    13321 caccgatatt ccggtagcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc
    13381 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cggcgttcgc
    13441 accgcaaaac tgtacggcgg tagagctaat ggcgaaaacg ctgcgtgaaa gtgcggaacc
    13501 tgtcacgatt gtgtctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc
    13561 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    13621 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    13681 ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag cacaaatcca
    13741 cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    13801 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggtgcacc
    13861 gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta ccactgttga
    13921 gcgctgggtt ggcgtggaaa cacaggggaa atatagccag ggtatgacgg ttgttgatta
    13981 ttattatctg actggcaata aaccgaatgc cactgtaatg gtcgatgttg atcgtcaggg
    14041 ctttgttgat ttactggctg atcgtctgaa attttacgct taacacacgt cattgattaa
    14101 gatcaccacg cgttacctgg gtggtcttaa tctgtttatc tctttttctt tcaagatata
    14161 aatgcaatgg ataatgcaga actcgccatt ggtatcgatc tcggcactac caatagttta
    14221 attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta
    14281 acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta
    14341 tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagt
    14401 aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc ctctttggta
    14461 ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa agatgtggtg
    14521 atctccgttc cggcttattt cagcgatgaa caacgcaagc atacccgttt agcagcggag
    14581 ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc gatggcatat
    14641 ggcctgcata cgcaacaaaa tacccgctcg ctggtttttg atctcggtgg cggcacgttt
    14701 gacgtcacgg tgctcgaata cgccacgccg gtgattgaag ttcacgcctc cgctggcgat
    14761 aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt aaagcgcgcg
    14821 gatgtcgcca ggaccacgct taacgagagt gaactggcag ccttgtacgc ctgtgtggaa
    14881 gcggcaaaat gtagcaatca atcgccattg cacattcgct ggcagtacca ggatgaaacg
    14941 cgggagtgcg aattttacga gaacgaactg gaagatttgt ggttgccgct gctcaatcgc
    15001 ttgcgagtgc cgattgaaca ggcgttgcgc gatgcacgtc tgaagccgag tcaaatcgac
    15061 agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat cgccgtgcgt
    15121 ctgtttggca aattaccgta tcaaagttac gacccgagca ccattgtcgc gctgggcgca
    15181 gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaggtgat cctcactgat
    15241 atttgccctt actcgttggg cgttgaagtt aaccgccagg gcgtttccgg cattttctcg
    15301 ccaattattg aacgaaacac cacagtaccc gtgtcgcgtg tagaaactta ctcgaccatg
    15361 cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca caaagttaaa
    15421 aataacattc tggttgaatc cttcgatgtg ccgttgaaga aaaccggggc ttatcagtcg
    15481 attgatattc gctttagtta tgatatcaac ggcttgcttg aagttgacgt gcttctggaa
    15541 gatggcagcg ttaagtccag agtcattaat catagcccgg taacattgag cgcgcagcag
    15601 attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga tatgctcatc
    15661 aaccgcacct ttaaggccaa actggaagag ttgtgggcgc gggcgctggg tgacgagcga
    15721 gaagagattg gccgggtgat caccgatttt gatgcggcgt tgcagtcaaa cgatatggcc
    15781 cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac ctcataatca
    15841 cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac acatcgtcaa
    15901 ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt taaactgatt
    15961 ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc ttcatggttt
    16021 tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt cgtcaacagc
    16081 atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct tgtattaaga
    16141 cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc tacccgatac
    16201 aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt ccaccacgcc
    16261 catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg cgataatttt
    16321 ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc aggaacatat
    16381 ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat cactaataac
    16441 gcgaaataat ccgggcaatg actcttgctc cactgcaaca acaaattatc agcttgtgta
    16501 gatgggtaac tttcccggta cgcgcagaga tcggcaagca ggctttcccc ctcgccgcag
    16561 tacaaccttt gagcacacaa gtaatatcgg gcatcttcgc tttctggttc ttccatttgc
    16621 cactgtttgg ctacaggaat gagttccgcc atacctgttt gcaccgagct gtaccagcgc
    16681 aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggaccttc catcgccaaa
    16741 aacgcagtga cgtagtcagg atggtaacgg cagcaacgtt ccagcgcgaa gtaatactca
    16801 acagtctggt tctgcaacgc aacgggatat tccagaaggc taaggaaatc gaaacaatca
    16861 ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc ttcgctgtca
    16921 ttaaacgaat gccagttcaa acgctctgcc agcaaagaag cacaatttaa tgaaagatat
    16981 cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc ctccatgttc
    17041 caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg atcactttct
    17101 gaatccagta atgtcctgaa tgcacgcagt atttcatgtt cagcggcggc atctttttct
    17161 tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc ataggcctgg
    17221 cgtaatgctt taaacccctg cggatcggtt tcgggatgac ataacggtaa gcgagcaaga
    17281 taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat ttgccaacat
    17341 gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg acagttaatg
    17401 gctgccaggg aagcgtggca tagaaacgat cgcttcccgc tgccagctct tcgaacatcc
    17461 gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa gtgttgtctt
    17521 cgcactgaga agattgccag tagagacgtc ctaacgaata ggcggaaaaa tagtgcatcc
    17581 agctgtgata gtaatggtga gctcgtacat agacgcgcga ttgcagccat aaactatctt
    17641 cttccgtcag ccagttgttg agcacaccga cccgtgacag aaaccccatt cgaacataat
    17701 cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg aattgggtat
    17761 aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca ggggaagagc
    17821 gatgccatgt cagatataag ccagctaaat cattggcatg accatcatca gtcatctgat
    17881 aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag agatcgaatt
    17941 cttttatacc ggtatacatt tttggcgagt catgcgcggg tttgcaaccc tgattaagta
    18001 tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc atttgttaat
    18061 aatcccttaa gtgcattaac tgactacttt ttattattga tgagtataaa cttgacgctt
    18121 tcaaaataaa aagaaaatcg aagtattcac atatgaataa aaaattaatg tatatattcg
    18181 caatatttat agttgcagca attacctgta ttagccaacc caagaaaaca acgttgcgtg
    18241 ataaagccat ggtgaattat gcctttgatt atttaagctc accaggcagt ctaccgttaa
    18301 ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa tatcgccttg
    18361 gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact
    18421 ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct
    18481 acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg tataaggaag
    18541 cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac agaaatggca
    18601 ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac
    18661 attacaaaaa tgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcaa
    18721 ccgaacagat ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt
    18781 ttaatgatgc ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc
    18841 tccggttcaa cagtttgctt ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata
    18901 attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat
    18961 gattcttcgg ttggttggtt cgcctgcact aattgtttgg caactaaata taatggccat
    19021 aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc
    19081 atctcgacca gtgaacgccc cagagcagag agtttttcat cgctgagtat ttcgtataaa
    19141 tgaaaacgcg tacgggcgct gtcgccgggg atataaagta gcggttttcc ctgatcgtta
    19201 accagttcac gagcttcaat catcatcacc actaacgggt aataatcgac ccgatgctga
    19261 cggcaccatt gcaatattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtca
    19321 caaagttctg ccagcaagct ttcgccttct ccacaataga cgcgttgtgc gtattcataa
    19381 taacgcgcat cttcattatc agattcagct tcccgccacg accgggcgta atccaggagt
    19441 tcgggaattc ccaggcgacc tgcgctatac cagcggagta aatttctgtg cagttttgca
    19501 tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc
    19561 gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg cacggcaacg
    19621 ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag
    19681 gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt
    19741 tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac
    19801 agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat
    19861 cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc acgaaaggcg
    19921 agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc
    19981 gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga
    20041 tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg
    20101 tcagtcgttt cctctatttc gagaatcttc cagcaatttt tcataggtcg ctcatatcct
    20161 taagcgtttc cgggcatgca gaatagtaag ccaatggttg ccaggacaag gtagggtaat
    20221 acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt
    20281 tacgcgctaa gaattcgcgt aataactgca gatgatcatc ttcgggagac tgccaatatt
    20341 gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt acgataataa cgtagagccc
    20401 gtagatgaat gcgcgactgg atccatagac tttcttcttc gctcaaccag ttattaagta
    20461 cgcccattcg gcttaaaaaa cccatccgga cataatccca ggctttaatt cctccctcac
    20521 cacagcattc ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt
    20581 cagcaagata gtcacgccat tcacatggcg aataacgaaa ccagcggtga taaaaccctg
    20641 ccaaccgagc ggcatgaccg ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac
    20701 gggcatcaat tccccaggag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg
    20761 gtgatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa
    20821 acaatatttt ttgcgactcc atgtccatat aatttatcct taaatattca ttaaatgaat
    20881 attagccgca ttgcgaggct cgtttattta tacttttctg acgcgacata atacgcataa
    20941 aataaggctt acgcaaaaat ggaacacata aaaatataat atgttttatc tgcaaccgta
    21001 aggaaatgat aatgattttc acgtcaagtt gctgcgataa tttatcaata gatgagatta
    21061 tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc
    21121 gcgatagcgc aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg
    21181 ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa
    21241 gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc
    21301 atactttcgc ttccaacgcc cttggctgga cactggatcg tggagaagcc cccaattata
    21361 aagaagcggt tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata
    21421 atcttgggtg gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt
    21481 tctggtacaa acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc
    21541 tttatgaaga cggaaaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga
    21601 aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg
    21661 gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc
    21721 aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg
    21781 agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca
    21841 ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc
    21901 gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg
    21961 aaattcgtga gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc
    22021 ggcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc
    22081 tggaactggt catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg
    22141 agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga
    22201 taaaccccat gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg
    22261 gtgatatctg ccagctcctg ccataagctt tctttaatca cccgtataaa gacgctgtca
    22321 gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca
    22381 gctcgcgtca gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg
    22441 cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt
    22501 gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg
    22561 ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact
    22621 ctgtaaatta atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg
    22681 ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat
    22741 ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag
    22801 agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag
    22861 acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac
    22921 acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc
    22981 tgggcaaaaa cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg
    23041 cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga
    23101 aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta
    23161 cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg
    23221 tatataagaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg
    23281 aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc
    23341 cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac
    23401 tggatcactg gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag
    23461 gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc
    23521 gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc
    23581 agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca
    23641 aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag
    23701 ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg
    23761 agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg
    23821 cctctaaata cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag
    23881 atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg
    23941 gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg
    24001 gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg
    24061 gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc
    24121 agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag
    24181 cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca
    24241 ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca
    24301 aagaaggtat gctggattcc gaagcggcta actactggct gccggtggat atctacattg
    24361 gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc
    24421 gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg
    24481 tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg
    24541 ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag
    24601 gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg
    24661 acccgcaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg
    24721 cttctccggc tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct
    24781 tcctgaaacg tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac
    24841 tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga
    24901 tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga
    24961 ttatggagct gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc
    25021 tgatgcagga agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct
    25081 gcttcacgct gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg
    25141 ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta
    25201 aagtccgtgc caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg
    25261 ctggccagga acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt
    25321 acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata
    25381 tctggcaaca ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct
    25441 gcgtgatacc acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc
    25501 gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct
    25561 tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc
    25621 gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac
    25681 ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagtg ccaaagtctt
    25741 ccgttcgttc tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat
    25801 cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg
    25861 tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac
    25921 gcctgcacgc gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc
    25981 cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg
    26041 ttattgcagg aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa
    26101 caccacactt tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag
    26161 gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg
    26221 aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg
    26281 ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct
    26341 cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc
    26401 tgggttgctg cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg
    26461 ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg
    26521 ctgctctggc cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc
    26581 gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg
    26641 ttgcatattc ttcagcaact gcgtctggaa ggcagcgaac cggttatttt gttgcgcaca
    26701 ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg
    26761 cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg
    26821 ttaaatcgct taagtcagac gcagttacgt caggccgtgc aactcctgac acgaacggaa
    26881 ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt
    26941 ctgttgtgcc ataaccccct ggcggacgta tttatcgacg gttgatatga aatctttaca
    27001 ggctctgttt ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac
    27061 gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca
    27121 tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc
    27181 cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac
    27241 tgcgcaaaca ctgaaagagt ggcggcagga acaagggccg gacgtgccgc tggcgtttat
    27301 tattggtcag gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga
    27361 caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca
    27421 ataccagcaa tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc
    27481 tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg
    27541 cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta
    27601 cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg
    27661 tggttaattc gtcatggcga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg
    27721 cccacacccc tgaccgcacg cggtattgag caagcgcaaa atctgcatac gctgctacat
    27781 ggtgtttcct ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcgcga
    27841 ctggttctca gtgaccgcca gctccccgtt catatcatcc ctgaactcaa cgaaatgttt
    27901 tttggcgact gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat
    27961 agcgcgtggt gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca
    28021 ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat
    28081 attttagtcg tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg
    28141 cctgccgagg ctatgtggca ttttcgcgtt gaccaggggt gttggagcac cattgatatc
    28201 aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca
    28261 tgacttttct gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg
    28321 atattctccg cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa
    28381 aatggcgatg caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg
    28441 gcgacaattg tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat
    28501 ttgattcacc cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa
    28561 aattgatgac ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat
    28621 caccgactgc atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga
    28681 ccacgttgtg caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa
    28741 cagcgccgac tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga
    28801 gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt
    28861 cgccgtggga acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg
    28921 ttttccgaaa gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa
    28981 tgcggacatc aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa
    29041 aaaccgcatt gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc
    29101 tgagctggaa cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga
    29161 agggttgtcg cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cgacgcttac
    29221 cctcccccat ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat
    29281 caccaccaac catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg
    29341 gatgaaacta cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg
    29401 ggcgctggtc gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta
    29461 taatctgcaa attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa
    29521 gctggtgcct atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc
    29581 cctcaaccgc actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca
    29641 aacgctggac gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg
    29701 aaaagagcgc gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga
    29761 agtacaagta gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg
    29821 ctataaacgt cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc
    29881 gaaaatcaac gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc
    29941 ggcaacgcat gatatcggta agctgggcat tgagcgttac tatgaagacg tggtgcacgg
    30001 tcagaccggt tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa
    30061 agaagtacca ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca
    30121 atatattgaa acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac
    30181 aggtggggtg ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg
    30241 tatctccagc aaagattatt ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg
    30301 cgccacacag ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc
    30361 attgagcgcc ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact
    30421 gccaggttcg gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt
    30481 cacaagatcg ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg
    30541 gatcgatcgc ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga
    30601 cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa
    30661 aaaaccgtgg tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc
    30721 gaccccaatc cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt
    30781 tccgcatttg ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca
    30841 tgaaccgccc gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta
    30901 cggtgttgct aaccgcccta acggtacggc gcataaatac tttgccagcg caccgtacaa
    30961 aattgcggcg aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa
    31021 tgcgcacaaa attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata
    31081 caacaatccg caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt
    31141 tggtacgctg atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct
    31201 gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg
    31261 aataaaaaaa cattctggga taaagtccat ctcgatccca caatgctgct gattttactg
    31321 gcattgctgg tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg
    31381 atggagcgca aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa
    31441 attcctccac gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg
    31501 ctggtggcgg tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc
    31561 ggtattgttc gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg
    31621 cgctttatca accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg
    31681 ctgatattta tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc
    31741 gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc
    31801 gtcgcagtag tgctggtggc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat
    31861 taccagcgcc agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcgggc
    31921 tatcacatta ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg
    31981 ctgcacggca ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc
    32041 gcggtactgg cggaagagct gggattagtg ggcattttga ttctgctcgc tctctacatt
    32101 ctgctgatca tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc
    32161 atggctggtg gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta
    32221 agcggtattc tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg
    32281 ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg
    32341 ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc
    32401 ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc
    32461 gcagcctgcg gtatgtaacg gccccatagt tgaaattagc ggggcggatc cgcgtttcga
    32521 accactgaac gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt
    32581 gcaggatccg tctcgattta gccaggcggg actggcggca atctatgatg ccgaaccagg
    32641 cagtaacctg acggcctctg gcgaagcttt cgatccgaca cagctgacgg cggcccatcc
    32701 aacgcttccg atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt
    32761 ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc gttatttcgc tttctcgcgc
    32821 ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt
    32881 cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac
    32941 ttacgccctg ccagcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg
    33001 cccgcagggt gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg
    33061 cgcgccggta accagtagcg gtttcctcgg cgctccaacg accttagcgc ctggtgtact
    33121 ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt acagctccgt cgacaacgcc
    33181 tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt
    33241 tatggtgcaa gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact
    33301 gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca
    33361 gcttggccca ttcgccagca aagccgaagc cagcaccttg cagcaacgtt tgcaaaccga
    33421 agcccaatta cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt
    33481 caatttctgt aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc
    33541 atttgctata gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga
    33601 ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag
    33661 cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac
    33721 cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg
    33781 aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg
    33841 ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca
    33901 acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac
    33961 cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg
    34021 atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga
    34081 tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg gtacatggtc
    34141 tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc caggcattga
    34201 tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta
    34261 ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca
    34321 aaaccggaca cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga
    34381 tgcgcttgat ttctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta
    34441 aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta
    34501 aagagttcgc ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg
    34561 ataaagacgt gtacctgacc attccacgtg gtcgcatgaa agatctgaaa gccagctatg
    34621 tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca
    34681 acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc
    34741 cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt
    34801 ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta
    34861 aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat
    34921 gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg
    34981 gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg
    35041 tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac
    35101 tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga
    35161 tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc
    35221 ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg
    35281 ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct
    35341 cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc
    35401 tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa
    35461 tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc
    35521 cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg
    35581 aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag
    35641 cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg
    35701 gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc
    35761 tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat
    35821 cacaatggaa acccgaagcg acgactaata atattgttcc acgtttactg gaaaatattt
    35881 tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat acctcaatgg
    35941 cccaatttac actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt
    36001 ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc
    36061 ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta
    36121 gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat tggagtaagt
    36181 gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca gaaggtaaac
    36241 cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg
    36301 tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa
    36361 tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag
    36421 gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc
    36481 ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa
    36541 ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta
    36601 aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac
    36661 tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc
    36721 aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac
    36781 tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt ctgttaccgg
    36841 aagagcaaaa tttcagcggt ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa
    36901 tcaatctcac cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt
    36961 tagcgattat tcccagccgt ttttataacc tgtttagccg ctgctggccg ctggaaaaac
    37021 tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca
    37081 gcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa
    37141 gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt
    37201 tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg
    37261 ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta
    37321 attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa
    37381 taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa
    37441 gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc
    37501 cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc
    37561 tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct
    37621 atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat
    37681 gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt
    37741 atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc
    37801 attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc
    37861 cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac
    37921 cataacctgg aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac
    37981 tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct
    38041 ggtctgatgg tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg
    38101 cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac
    38161 ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg
    38221 gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat
    38281 ttgcaggcga aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat
    38341 aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag ggagcgtcct
    38401 gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt
    38461 ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa
    38521 ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa
    38581 ctactagcag tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc
    38641 aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata
    38701 gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg
    38761 cgcaaagcga cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg
    38821 cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa
    38881 gggatctata atccggcttt gaccgatatt tttgttcccg cactcccccg ccgccaccat
    38941 ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccaatgat gctctttgag
    39001 cggcccgcga acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc
    39061 cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccctc
    39121 cacctcgagt aacggagcga tttcattacc agcatcaaca cggcgtgatt cctgaatggc
    39181 aaatgcatca tagagatgca atttggcata acgggcgacg atgtttcctg cctgtagcgc
    39241 caccagcata ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt
    39301 catcatgtta cgtttacttt ctcgccgtaa acgtccgagg agttcgcctt ccagcagctg
    39361 tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag
    39421 gacgaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc
    39481 gttcttttcc cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc
    39541 ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta tcactcatac
    39601 aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg
    39661 gtacgggaag cgtggcgaga tggctgttaa gtatgcgatt taacccgctg catcaggcga
    39721 ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga atgggattcg
    39781 cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat
    39841 tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag
    39901 agggccgctt tggctgggcg ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca
    39961 tgacagcact ggctttctgg ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa
    40021 acccgctgat taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt
    40081 gcagcagaag ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca
    40141 agagttttaa aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg
    40201 tcttccggag taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta
    40261 atcttagaca tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttacc
    40321 agtacaccaa ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg
    40381 cgttattttt gatgttactt attttagcta ttgattttaa agaagttact aaaacttcat
    40441 ttgtctcaaa actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc
    40501 ggaatgtagg tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga
    40561 accgggagag ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga
    40621 cctaaatgaa aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca
    40681 atcggttccc atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt
    40741 cctttttcat cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga
    40801 tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa
    40861 tcataatgtt caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatccac
    40921 tcatctgcgt tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag
    40981 atagcgacat atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta
    41041 ctagcatagc aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg
    41101 aatagctgct ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag
    41161 atgatgcgtc gttttctgcg atgggaatag tcaaaaaa
//
LOCUS       DAZGCS010000037        40297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGCS010000037 DAZGCS010000000
VERSION     DAZGCS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.037"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..344)
                     /gene="chbB"
                     /locus_tag="ACHK01_002002"
     CDS             complement(24..344)
                     /gene="chbB"
                     /locus_tag="ACHK01_002002"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO8096958.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(643..981)
                     /gene="osmE"
                     /locus_tag="ACHK01_002003"
     CDS             complement(643..981)
                     /gene="osmE"
                     /locus_tag="ACHK01_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO8096959.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1183..2010
                     /gene="nadE"
                     /locus_tag="ACHK01_002004"
     CDS             1183..2010
                     /gene="nadE"
                     /locus_tag="ACHK01_002004"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO8096960.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2240..3127
                     /gene="cho"
                     /locus_tag="ACHK01_002005"
     CDS             2240..3127
                     /gene="cho"
                     /locus_tag="ACHK01_002005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO8096961.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3087..3662)
                     /gene="ves"
                     /locus_tag="ACHK01_002006"
     CDS             complement(3087..3662)
                     /gene="ves"
                     /locus_tag="ACHK01_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO8096962.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFHWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3865..4350)
                     /gene="spy"
                     /locus_tag="ACHK01_002007"
     CDS             complement(3865..4350)
                     /gene="spy"
                     /locus_tag="ACHK01_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO8096963.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4680..5648)
                     /gene="astE"
                     /locus_tag="ACHK01_002008"
     CDS             complement(4680..5648)
                     /gene="astE"
                     /locus_tag="ACHK01_002008"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO8096964.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5641..6984)
                     /gene="astB"
                     /locus_tag="ACHK01_002009"
     CDS             complement(5641..6984)
                     /gene="astB"
                     /locus_tag="ACHK01_002009"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO8096965.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6981..8459)
                     /gene="astD"
                     /locus_tag="ACHK01_002010"
     CDS             complement(6981..8459)
                     /gene="astD"
                     /locus_tag="ACHK01_002010"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO8096966.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8456..9490)
                     /gene="astA"
                     /locus_tag="ACHK01_002011"
     CDS             complement(8456..9490)
                     /gene="astA"
                     /locus_tag="ACHK01_002011"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO8096967.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDLWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9487..10707)
                     /gene="astC"
                     /locus_tag="ACHK01_002012"
     CDS             complement(9487..10707)
                     /gene="astC"
                     /locus_tag="ACHK01_002012"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO8096968.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11153..11959
                     /gene="xthA"
                     /locus_tag="ACHK01_002013"
     CDS             11153..11959
                     /gene="xthA"
                     /locus_tag="ACHK01_002013"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO8096969.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12126..12836
                     /locus_tag="ACHK01_002014"
     CDS             12126..12836
                     /locus_tag="ACHK01_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8096970.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLASEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12841..13518
                     /gene="ydjY"
                     /locus_tag="ACHK01_002015"
     CDS             12841..13518
                     /gene="ydjY"
                     /locus_tag="ACHK01_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO8096971.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13533..14240
                     /locus_tag="ACHK01_002016"
     CDS             13533..14240
                     /locus_tag="ACHK01_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8096972.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14240..14788
                     /locus_tag="ACHK01_002017"
     CDS             14240..14788
                     /locus_tag="ACHK01_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO8096973.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14798..15964
                     /locus_tag="ACHK01_002018"
     CDS             14798..15964
                     /locus_tag="ACHK01_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8096974.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            15937..17472
                     /locus_tag="ACHK01_002019"
     CDS             15937..17472
                     /locus_tag="ACHK01_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO8096975.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17472..18125
                     /locus_tag="ACHK01_002020"
     CDS             17472..18125
                     /locus_tag="ACHK01_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8096976.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18192..19499
                     /gene="ynjE"
                     /locus_tag="ACHK01_002021"
     CDS             18192..19499
                     /gene="ynjE"
                     /locus_tag="ACHK01_002021"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO8096977.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19508..20128)
                     /locus_tag="ACHK01_002022"
     CDS             complement(19508..20128)
                     /locus_tag="ACHK01_002022"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8096978.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20215..20622
                     /gene="nudG"
                     /locus_tag="ACHK01_002023"
     CDS             20215..20622
                     /gene="nudG"
                     /locus_tag="ACHK01_002023"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO8096979.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(20588..20860)
                     /locus_tag="ACHK01_002024"
     CDS             complement(20588..20860)
                     /locus_tag="ACHK01_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO8096980.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            21096..22439
                     /gene="gdhA"
                     /locus_tag="ACHK01_002025"
     CDS             21096..22439
                     /gene="gdhA"
                     /locus_tag="ACHK01_002025"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO8096981.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22556..23597)
                     /locus_tag="ACHK01_002026"
                     /pseudo
     CDS             complement(22556..23597)
                     /locus_tag="ACHK01_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(23725..25686)
                     /gene="topB"
                     /locus_tag="ACHK01_002027"
     CDS             complement(23725..25686)
                     /gene="topB"
                     /locus_tag="ACHK01_002027"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8096982.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25691..26734)
                     /gene="selD"
                     /locus_tag="ACHK01_002028"
     CDS             complement(25691..26734)
                     /gene="selD"
                     /locus_tag="ACHK01_002028"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO8096983.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26851..27402)
                     /locus_tag="ACHK01_002029"
     CDS             complement(26851..27402)
                     /locus_tag="ACHK01_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO8096984.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27563..29419
                     /gene="sppA"
                     /locus_tag="ACHK01_002030"
     CDS             27563..29419
                     /gene="sppA"
                     /locus_tag="ACHK01_002030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO8096985.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29586..30602
                     /gene="ansA"
                     /locus_tag="ACHK01_002031"
     CDS             29586..30602
                     /gene="ansA"
                     /locus_tag="ACHK01_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO8096986.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETICKAMSQNLRGEL
                     TPDD"
     gene            30613..31254
                     /gene="pncA"
                     /locus_tag="ACHK01_002032"
     CDS             30613..31254
                     /gene="pncA"
                     /locus_tag="ACHK01_002032"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO8096987.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31415..31687)
                     /locus_tag="ACHK01_002033"
     CDS             complement(31415..31687)
                     /locus_tag="ACHK01_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO8096988.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31729..32142)
                     /gene="msrB"
                     /locus_tag="ACHK01_002034"
     CDS             complement(31729..32142)
                     /gene="msrB"
                     /locus_tag="ACHK01_002034"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO8096989.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32484..33479
                     /gene="gapA"
                     /locus_tag="ACHK01_002035"
     CDS             32484..33479
                     /gene="gapA"
                     /locus_tag="ACHK01_002035"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8096990.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33563..34447
                     /locus_tag="ACHK01_002036"
     CDS             33563..34447
                     /locus_tag="ACHK01_002036"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO8096991.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34498..35352)
                     /gene="yeaE"
                     /locus_tag="ACHK01_002037"
     CDS             complement(34498..35352)
                     /gene="yeaE"
                     /locus_tag="ACHK01_002037"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO8096992.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35442..36188)
                     /gene="mipA"
                     /locus_tag="ACHK01_002038"
     CDS             complement(35442..36188)
                     /gene="mipA"
                     /locus_tag="ACHK01_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO8096993.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36624..38558
                     /gene="yeaG"
                     /locus_tag="ACHK01_002039"
     CDS             36624..38558
                     /gene="yeaG"
                     /locus_tag="ACHK01_002039"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO8096994.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWSSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38671..39954
                     /locus_tag="ACHK01_002040"
     CDS             38671..39954
                     /locus_tag="ACHK01_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO8096995.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            40101..>40297
                     /locus_tag="ACHK01_002041"
     CDS             40101..>40297
                     /locus_tag="ACHK01_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8096996.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLT"
BASE COUNT         9784 a        10537 c        10085 g         9891 t
ORIGIN      
        1 ttgacgggaa aatttaaaat aaattaattt gctgcggctt ttttaatcgc tgcaaccgca
       61 gccttaagca cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc
      121 ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat
      181 aacacgacat cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata
      241 atgaccggaa cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagaggta
      301 gacatgcccg cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg
      361 acgattatct gtcagccaga cactccgcaa gccttaacct gcttccatgc tctgggtaac
      421 ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca
      481 atagtatttc tttgacctta taaattgttt ctccttgacc tgtggttatg accctcttca
      541 catttcgggc aaataattcg cgaagataat taagaactca ggccaaaaaa aaccggcgca
      601 atggccggtt tccgttgtta ctcaagcttt cagacgaatt gattacttcg cagcctgtgg
      661 atcagtgtcg tattcagcac aggtctgata accggagttg atgacatgtc cggtatcatc
      721 taacgcgaca aagtaggttt ctgctttacc atcacgttga cccaggatgt aggtctggca
      781 agtaccgcga gcatggatca tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac
      841 ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa actggtcttt
      901 agtacgatca taagccgtac aacccgccag catggttaat accgccgctg cactcagaat
      961 tcctgccata ttcttgttca tattccgtcc tcttgtttat cagcgtgtta gataagcctg
     1021 gaatacattg ggcgcttttt caagcccgtg aacgaaacag ctccgctttc agaggattcc
     1081 tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt
     1141 tagctttaag gaagttttgt cttttctgtc tggaggggtt caatgacatt gcaacaacaa
     1201 ataataaagg cgctgggcgc aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt
     1261 gtcgattttc tgaaaagcta cctgcaaact tatccgttca ttaaatcact ggtgctcggg
     1321 atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt gccagatggc gattaatgag
     1381 ctgcgccagg aaactggcaa cgaatcactg caatttattg ccgtacgcct gccctatggt
     1441 gttcaggccg acgaacaaga ttgccaggat gccattgcct ttattcaacc ggatcgcgta
     1501 ttaaccgtta atatcaaggg cgcggtattg gctagcgagc aggcattgcg ggaagcaggc
     1561 attgaactga gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa
     1621 tatagcattg cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc
     1681 attaccggat tcttcactaa atatggtgac ggcggtacgg atattaatcc gctgtatcgt
     1741 ctcaacaaac gccagggtaa acagttgctg acggcattag gttgcccgga acacctttat
     1801 aagaaagccc caacggccga tctggaagat gatcgccctt ctctgccaga tgaagtggca
     1861 ctcggcgtga cctatgacaa tatcgacgac tatctggaag ggaaaaacgt acctgaacag
     1921 gtcgccagaa caatagagaa ctggtatctg aaaaccgaac ataaacgccg tccgccaatt
     1981 accgttttcg atgatttctg gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa
     2041 gagtaatttg ttcacgccgg atgcggcgtg aacgccttat tcgacctaca aaactatgca
     2101 aattcaatac attgcaggag tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa
     2161 cctttgtcat caacccgcct cttttttaat cgcctcccgc ctgttacact ggatagataa
     2221 ccagcattcg gagtcaacag tggtacggcg tttaacttct ccgcggctcg aatttgaagc
     2281 tgcggcaatt tatgaatatc ccgaacattt acgttcattc cttaatgact tacccacccg
     2341 accaggggtg tatctgtttc atggcgaaag tgacaccatg ccgctctata tcggcaaaag
     2401 cgttaatatc cgcagtcggg tgctttctca tttacgcacc ccggatgaag ccgccatgct
     2461 acggcaatcc cggcgaatta gctggatctg caccgcaggc gaaatcggcg ctctgctcct
     2521 tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat aaacggttgc gccgcaatcg
     2581 ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt
     2641 ggatttttca cgagccccca acctgtttgg cctgtttgcc aataggcgcg cagctttgca
     2701 agcattgcag agcatcgctg atgaacaaaa actttgttat ggcctgttgg gactggaacc
     2761 gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg
     2821 cggtaaagag agccatgagg aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg
     2881 ggtggtgtgt tggccttggc aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac
     2941 tcaatatcat attattcaaa actggctgtg gctgggggcg gttaattcgc tggaagaagc
     3001 gacaacgtta attcggacac ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa
     3061 gccgctgctt tccggtaact atgaaattac tgaacttgat ccggcgaatg accagcgagc
     3121 cagttgattt cactgaacaa cagtttacct tgcggttgta aaagccgcag ggtgtgcctg
     3181 ccatcgaacc agcacgcgcc ctggtcggta gtgagcaatt tatcgcccag ttgccacgcc
     3241 ccgttaatca caaacaccac cccgccgcgt gagccaaagg tagtaaacgt acgttcggca
     3301 attctgactt tcgccttaca gacatccagg cgtgtcatga tgttgaaatc catcgacatc
     3361 tgccctgccg tcagtttcgc ttttaccacc tggtccgctg caaaggcaaa aggctgtaac
     3421 ggttttaagg tatggttaaa gcggtctgcg ctttcaagga acatctcgcc gccttccagc
     3481 aacgtcacta tcctttccat gccgggaaac aaagaaaact ccccattcgc cgcgatggat
     3541 gcaatgctgg cacgccagtg aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc
     3601 gtttcaccgg cagcgtttcg ccacagattc accgacattt tacgcatatc aaagtattcc
     3661 atactcgctc ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg gatggttatc
     3721 ggcaactcgg gtgatttacg ttagtggtga tcaggctttt tttacaactg tttgagaaga
     3781 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg
     3841 gtcttaagtt ttttggctga aagattattc agcagttgca ggcattttac cttttgccgc
     3901 tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct gttccggcgt
     3961 caggatgttg taaattttgt tctgggtttc catgtgcgcc agcatgttag ctttgcgctg
     4021 ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat
     4081 gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt cacgctggcc
     4141 tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat
     4201 catgtcctga tgcggaccga acttgccttt gtggtgcatc atcggcttcg cgtcagccgg
     4261 tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag ccagggtaga
     4321 ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca gttatttatt
     4381 acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact ttagcgtcaa
     4441 ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc caattatgga
     4501 gaaaaaaaga aaatttgatg gagagtgatg agagaataat acaacacgat gattttgcag
     4561 agattatgaa gaactatacc ggatgactgg tgataaataa agcaaataac caggattaat
     4621 ctgtattaat ttataagaaa gcaacttaat acccgcagaa tgatttctgc gggtaagtat
     4681 tagcttattt tttcgagcat taatcccgcg cgtaatccca acgctaccaa cggattaggg
     4741 aataacacat actctacatc atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc
     4801 aatgttcctt tctcaaacgg cataaaattc agcgtgtcac ttgccatatg catttcgaag
     4861 gacggcgagt ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg tgttctcacg
     4921 ataccgacac tctcaccaga tagcagcgca gcaattgcgc tggcagttac tgcaaactgg
     4981 cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt ccagcgtaca ggccagcgcg
     5041 ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag gttcctgatg gaacaccagc
     5101 gcctccagcc ccgccgcacc cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt
     5161 tgcggtaata caccgaactg cggatgcaag gagccacgaa ttgcggtatg tagatcaagg
     5221 tgccagcgca cagattcttt gcccaggtca taaaaatctt ccaggcactg ttccagttcg
     5281 cgcgcccgac aggtttctcc gctttcagca aatagctgcc aacgaccgcc aaacattcga
     5341 ttcatatcgc tatggcaata acgtttccct tgcttcagcg caggaggatt cccgaggatc
     5401 accagcaacc gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc
     5461 agcatctcca caggtgccgt ctcattaccg tgtattcccg ctgaaatcac cagtgcgcct
     5521 tgcggtggcg ttaatggcgt cagttccagc acaccatcgc ccagccagcg ccagcgaacg
     5581 ccgttgattt ctcgctcggt gataaccggt tttttacccg ttaaggtcag agcaagaaaa
     5641 ttatccattg ccccctccct cgcgctggaa cggataaacc gaaccgagat tcagtaattg
     5701 gctcaataca tccagtgctt cccgccctcc gcgcagcaat tgcgggtcgg ccagatcggc
     5761 agcagtaagg cgatcgcggt agtaacgatc cacccagtca ttgagcgcat taaacagcgt
     5821 atcgttcatc atcaccgccg gattcaccgc ccggcgttct tcttctgtca ataccacccg
     5881 caaccgcagg cacgccgggc cgccgccatt cgccatgctt tcacgtaaat caaagacttt
     5941 tagttcgcta atcgggttgt cagcggcaag gagttcattg agataacccc atactccggc
     6001 gtgttcccga cactcctgag gcagcaccaa catcatggaa ccatcatcgc ggctcaacag
     6061 ttggctgtta aacagatagg tagacaccgc atccgacacg gaaacctgag ttgccggaac
     6121 ttctatcgcc ataaaaccgt tcacccgcgc acgcaggttt gccagtaact gtgactggcg
     6181 agcgaacgcc tgttggtggc aaaacagcac ctggcggtta ctcacggcaa tcacgtcatt
     6241 atgaaaaacg ccctggtcga taacgtccgg gttttgctgg gcgaaaatca cctgttgggg
     6301 attcacctga ttcagccttg ccaccgcctc gctggcttcg cgagtctgtc gcgccggata
     6361 acgggaaggc cgggtatcat tgccttcttc tcgcccgtag acaaaaagtt gcatacccgg
     6421 ctcaccgtaa tgaccgccga gacgattgtg gtttgccgcc ccctcatcac cgagcaacgc
     6481 tacctgcggc aacgccgaat gaacgctaaa tttctcttcg tcgttaaaaa tcgcttttaa
     6541 aagtgattcg gtgacgtgcg cttccagcga gcggtgaaat ttattgttca ggttggcaac
     6601 ggtgagatgc actttgccat ccagcgtatc ggcagatggc gcgatcgttg ccgcattggc
     6661 tacccacatt ggcgaagcgg aactgacgct ggaaagccag tgcggtgcct ggcgtgcaac
     6721 tttttccagt acctgctcat cgctaccact gaatcccaac tgacgcagca ccggaataaa
     6781 cggacgctcg tgcggcggga tcacggcctg ggggaatccc gcatcggcaa gggctttcat
     6841 tttcagtaag ccctgttttg ccgcctgtcg cgggttagac acctgaaaac ggtgacgggt
     6901 agaggcttca ttaccaaacg acaggccagc gtaatgatgc gtcagcccta ccagcccgtc
     6961 gaaattaact tcccaggcgt tcatcgcacc acctcatcgg aaaaatccag cccggggtta
     7021 agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg ccataggcca tgcgcagtaa
     7081 tctgcggcat accaggcgct ggggcgatgg ttaccggatg caccaatgcc gccgaatggc
     7141 gcggtactgg cagcaccagt aagcggtttg ttccagttaa caatccccgc ccgcgcctcc
     7201 agcaacagtt gatcgaattt ttcccgctcg ggggaaacca gaccgcaaga gagtccgaag
     7261 cgagtgttat tcgccattag aatcgcttcc tcgaaagaat cataacgcca gacgcgcaat
     7321 aacggtccga acacctcttc atctggtacg ccagcaacgc ctgtcatttc aatgatcccc
     7381 ggcgtcagca acgatgtctc tgattgtaat aagcgcggcg caagcagggt tcgtccgccc
     7441 atcgcttcca gttgctgcca ggcagtaacc acctgctgtg cggcctgttc agaaatcagc
     7501 ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg gcgttaatcg ctggctgacg
     7561 gcaaccagac gagcaagaaa cgcatcgccc tgcgctccgc ttttgagcaa taaacggcgg
     7621 gcgcaggtgc agcgttgccc ggctgtgaca aacgccgact gaatggtcag atggacagcc
     7681 gcgtcgatat ccgccacctc atcgataatt agcggattat taccgcccat ctcaagggcg
     7741 agaattttct ccggctgacc ggagagctgg cgatgcagct ggtagccggt attggcgcta
     7801 ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca gcgcctgacc cgtttcacgc
     7861 ccgccctgca ccaggttcag tacgcccggc ggcaagccag cctgctgcca taaacgcatt
     7921 accgcttcgc cactccacgg tgtcagttcg ctgggtttaa agataatggt gttacctgcc
     7981 agcaatgccg gaacgatatg tccgttcggc aaatgaccag ggaaattata cggcccaaac
     8041 accgccagca cgccgtgcgg gcgatgtcgc aggctcgccg caccgtccgg catttcacta
     8101 cgctgctcgc cggtacgaac gtgatacgcc ttaattgata tcgcgatttt attgatcatc
     8161 gccgtcactt cggttgccgc ttcccagcgc ggcttacccg tttctctggc aataatcgcg
     8221 gttaattcgc ctttattcct ttccagcaat ccggcaaagc gttcgacaac gacctgacgt
     8281 tcagccaatg agagccgcgc ccagcgcgga aacgccgcac gggctgcgcg acaagcctgc
     8341 ccgacctgag cggcatcggc atcattgcct tgccataaca cctcgcccga taccggatta
     8401 cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca taaagtcatg
     8461 ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg atcccctgcg tggcatttga
     8521 gggcatccag ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg gtacgcgcca
     8581 gcaccacgcg gaaatggtga taattttcat tggcgaccag gcaggccgga aaatcgccct
     8641 gcgcaggctg cccttctgcc acttccacca gccgactttt acgaatggcg cgcacgcggt
     8701 cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg cggtaacgaa
     8761 aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc tgaccgatga
     8821 cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg atgtttcggc atcagttctg
     8881 caataaatgc cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa tccatcgaaa
     8941 agaagcgttt accgaggctt tgccagaacg gtgaatagcc gtgttcgtca atcaccccgc
     9001 gcatttcagc aaccactttg tcattaaact tgtcgcgaaa agccgccata aacataaagc
     9061 gcgatttcga cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg agaaacagcg
     9121 tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa cagcgtcggc aatgcgttat
     9181 agacattcag ctcttttgag gcgtgaacca acgtgccgac gcgatagttg taccagagat
     9241 cgttcagccc aaccgccacc tcaatggcac aaatccccgc cacggtgcct gtctcgctat
     9301 cttccagcac gaacacatag ccctgctcac ttttgggcag ttcgccttgc caggttttga
     9361 ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc gggaagcgac gtcaggccgc
     9421 cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc tgatcgctca acgggacgga
     9481 tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa agcgatccag
     9541 tccggtcgtc acctcttctt cgctgacatt gagcgcaggc gcaaaacgca ccacgttgcc
     9601 acccgcaatc agtaccatca cgcctgcttt cgccgcttcc tgagagatct gtttcgcttg
     9661 cccggcgtaa tcggcattca gtacacagcc aatcagcaaa cctaagccgc gaacttcact
     9721 gaacaaacca tagcggtgat taatagtatt aagacgctca acaaaccagt cgtgacgctg
     9781 tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc agcactttgc ctgccaccgc
     9841 cgaggccagc ggattaccgc cataggtggt gccatgagtg ccaacggtca tcacgcgggc
     9901 gcactcttcg gttgccaaca acgcaccgac cgggaaaccg ccgcccagcg ctttggcggt
     9961 agttaacaga tcaggcgtca cgccgtagtg catataggca tacagttccc cggtgcgccc
    10021 gacgccggtt tgtacttcat caaaaatcaa cagcgcattg tggcggttac acaattcacg
    10081 cagaccttgt aaaaacgcgt tgctggctgg caccacaccg ccttccccct ggatgggttc
    10141 gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg gcagagttaa tatcgttata
    10201 tgcagcatga cgaatatccg ccggcagtgg cgcaaaatcc tgtgaatagg ctggctgccc
    10261 acccgcactg acagtaaaca gcgtgcgacc atgaaacgca tttttgaacg ccacgatgcc
    10321 gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta gcgccgcttc
    10381 gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg cgtcgatcaa
    10441 ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat gccagaactt
    10501 actcgcctgt tcgttcagcg cttcacgcag ttccggatgc gcatggccca gcgcgttcac
    10561 cgcaatgcca cccgcgaagt cgatatactc tttcccctgc tgatcccaca agcgcgaacc
    10621 ttcgccacgt accggtataa agggtgccgg agcgtaaaca ggtatcatcc attcatcaaa
    10681 gttttcacgc gtaattggct gagacatagc gacctctaca gtaaataatt cgttatttat
    10741 atgttaacaa taagtaatgt ttgcgctgta aatgtagatt gcagggttcg tgccagccag
    10801 tgataaaagt gcataaacgg cggaggctaa ctggaaatca aggagttata accaaaccat
    10861 atgcatttaa agtgcatata aagtgaatac gtttgcgatg tgggtgaata aaaagaataa
    10921 aaaacgcaat gttatgcaga agtaaaatat aattctggaa ttgtgatcat tgacgaaatt
    10981 tactggaaat tactgcgcca ttctgacgca gcgcgcacca aaagcgggca ttttttgcgc
    11041 catcgttgac atcattaaca accatcgatc aaatcactta acaacaggcg gtaagcaacg
    11101 cgaaattctg ctaccatcca cgcactcttt atctgaataa atggcagcga ctatgaaatt
    11161 tgtctctttt aatatcaacg gcctgcgcgc cagacctcac cagcttgaag ccatcgtcga
    11221 aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa gttcatgacg atatgtttcc
    11281 gctcgaagag gtggcgaagc tcggctacaa cgtgttttat cacgggcaga aaggccatta
    11341 tggcgtggcg ctgctgacca aagagacgcc gattgccgtg cgtcgcggct ttcccggtga
    11401 cgacgaagag gcgcagcggc ggattattat ggcggaaatc ccctcactgc tgggtaatgt
    11461 caccgtgatc aacggttact tcccgcaggg tgaaagccgc gaccatccga taaaattccc
    11521 ggcaaaagcg cagttttatc agaatctgca aaactacctg gaaaccgaac tcaaacgtga
    11581 taatccggta ctgattatgg gcgatatgaa tatcagccct acagatctgg atatcggcat
    11641 tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa tgctctttcc tgccggaaga
    11701 gcgcgaatgg atggacaggc tgatgagctg ggggttggtc gataccttcc gccatgctaa
    11761 tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt ttgacgataa
    11821 ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat gttgcgtaga
    11881 aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg cccccgtctg
    11941 ggcgaccttc cgccgctaat ttagcagctc tcctggctca aactgggtca ggagaattaa
    12001 ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc tttgttatac
    12061 cgtcactgcg tttttagttg tctgaccact tctctattat caagtttgat ataggaaact
    12121 ccacgatgaa cgctgagcgt aactttctgt ttgcctgtct tatttttgcg ctggtcattt
    12181 acgctatcca cgctttcggt ttattcgatc tgctcaccga tttaccccac ttacagacac
    12241 tcattcgcca gagcggattg ttcggttata gcctctatat tctgttattc atcattgcca
    12301 ccctctttct gttaccagga agcatactgg tgatcgctgg aggaatagtt tttggcccgc
    12361 tcttagggac actactctca ttaattgccg ccacgctggc ctcctcgagc tcattcctgc
    12421 tggcgcgctg gctgggacgt gatttactgc tgaaatacgt gggccatagc cataccttcc
    12481 aggccattga aaaaggcatt gcgcgtaacg gtattgattt tcttattctg acccgcttaa
    12541 tcccgttgtt tccttacaat attcaaaatt acgcttacgg attaaccacc atcgccttct
    12601 ggccttatac ccttatttcg gcactcacga ccctgcccgg tattgttatt tataccgtga
    12661 tggcaagcga tctcgccagt gaaggcatta cgctgcgctt tattttacaa ctctgtctgg
    12721 cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact ctacgcccga cacaaacatg
    12781 tggatctgtc tgcttcgcgc cgcagcccac ttactcaccc aaaaaatgaa ggatagaacg
    12841 atgtcgcaac attattcagt gtcatggaaa aaaggactgg ctgcactctg cttactggct
    12901 gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg cggcaaaagt ggaatacgac
    12961 ggactttcga acagccaacc gctgcgcgtc gatgccaata accatacggt aaccatgctg
    13021 gtgcaaatta atggtcgttt cctcaccgac gtcactcgtc acggtattgt gtttaaagat
    13081 ggctccaacg gacataaatc gctgtttatg gcttatgcga ccccgaaagc attttatgaa
    13141 gccctgaaag aggcaggtgg tacgccgggc gaaaatatga cgatggataa taaagaaacg
    13201 actcttgtca caggcagcaa actggatatt tcggtcaact ggcaaggggc ggcaaaagcg
    13261 tattccttcg atgaagtgat tgttgacagt aatggcaaga aactggacat gcgctttggc
    13321 ggtaatttaa cggcagcaga agagaagaaa acgggttgcc tggtgtgtct ggatagctgc
    13381 ccggtcggca tcgtcagcaa tgcaacatac acttatggtg cggtggaaaa acgcggcgaa
    13441 gtgaaattca aaggcaatgc ctcagttctc ccggcggata acacgctggc aacggttacc
    13501 tttaaaatca ccgaataaag ccaggataaa ggatgatgaa gatacaatcg cgtaaaatct
    13561 ggtactaccg tataaccctc atcatcctgt tgttcgctat gctgctcgca tgggcgctgc
    13621 ttcccggcgt ccatgagttt atcaatcgca gcgttgcggc gtttgccgcc gtggaccaac
    13681 agggtataga acgctttatt cagtcttacg gcgcactggc ggcagttgtc tcgttcttgt
    13741 tgatgatttt acaggccatt gctgcaccgc tacctgcgtt tttgatcacc tttgccaatg
    13801 cgtcgctgtt tggcgcgttc tggggcggct tgctgtcgtg gaccagttcg atggccggcg
    13861 cggcgctgtg cttttttatc gccagagtga tgggccgcga agtggtggaa aaattaaccg
    13921 gcaaaaccgt gcttgacagt atggacggct ttttcactcg ctacggcaaa cacaccatcc
    13981 tggtctgtcg gttattgcct tttgtccctt tcgatccaat cagctatgct gccggtctga
    14041 cttcaatacg ttttcgctcg ttttttatcg ccaccgggct tggtcaatta ccggcgacta
    14101 ttgtttattc ctgggcgggc agcatgttaa caggcggtac tttctggttt gtcaccggac
    14161 tgtttattct gtttgccctg accgtggtga tttttatggc gaagaaaata tggcttgagc
    14221 gccagaagag gaatgactga tgggtttacc gccgcttagc aaaattcctt tcattttacg
    14281 tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg ctaagcccga ttcgctggtg
    14341 ggggcggatc ccgtttatct tttatttggt gtcaatgttt gttgggtggc tggagcgcaa
    14401 acgctcaccg ctcgatccgg tagtgcgatc gcttgtcagc gcgcgcattg cgcaaatgtg
    14461 cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc gccgagcgta ccggcagcaa
    14521 cgataaactg ctggcagtgg ctgactggcg gcaaagcccg ctctttagcg atgaagaacg
    14581 gctggcgctg gagtacgccg aagccgcaag cgtcacaccg ccaacagtcg atgatgccct
    14641 gcgtacccga ctggctgcgc attttgacgc tcaggcgctc accgaactga cggcattgat
    14701 cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg gacattcccg ctcaggggct
    14761 gtgccgtatt ccggaaaaac gttcttaagg agggaagatg cgccattgtg ggtggttgct
    14821 gggattgtta tcgctgtttt ctttggcaac acatgccagt gactggcagg aaattaaaaa
    14881 tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc ggcgataccg caattaaccg
    14941 ctatctcgac tgggttagcg gcgagatgaa aacccattac gctataaacc tgaagattgt
    15001 tcgtctcgcg gatgccgcag acgcggtgaa gcgcattcag accgaagctg ctgccggacg
    15061 taaaacgggc ggctcggtgg atctgctctg ggtgaacggc gaaaacttcc gcaccttaaa
    15121 agaggccaat ttattacaaa cgggctgggc ggagactctg cccaactggc gctatgtcga
    15181 cacacagctg ccggtgcggg aagatttttc agtgccgaca caaggtgcgg aatcgccctg
    15241 gggcggcgca caactgacgt ttatcgcccg ccgcgatgtt acgccacagc caccacaaac
    15301 gccgcaagcc ttactggagt ttgctaaagt caatcccggc acggttactt atccgcgccc
    15361 accggacttt accggcacgg cgtttcttga acagttgctg attatgctga cgccagatcc
    15421 cgccgcatta aaagaagcgc cggacgatgc gactttcgcc cgtgtcactg ctcccttgtg
    15481 gcaatatctt gatgcgctac atccgtattt gtggcgcgaa ggaaaggatt tcccgccttc
    15541 gcccgcgcgg atggatgctc tgctgaaagc cggcacattg cgcctgtcgc tgacctttag
    15601 ccccgcgcat gcgcagcaaa aaatcgccag cggcgatttg ccggcaagca gttacagttt
    15661 tggctttcgc gaggggatga taggcaacgt gcatttcgtc accattccag ccaacgcgaa
    15721 tgccagtgct gcggcgaagg tagttgccaa tttcctgctc tcacccgatg cgcaactgcg
    15781 taaagcagat cccgttgtct ggggcgatcc ttctgttctc gatccgcaaa aactgcctga
    15841 cgggcagcgc gaaatattgc aatcaagaat gccgcaggat ctgccgccgg tactggctga
    15901 accgcacgca ggatgggtga atgcactgga acaagaatgg ttacgccgtt acggtacgca
    15961 ttaatctttt tgctgtgggc gatggtggcg gtgatttatg caccgctgat cccggcagct
    16021 ctcacgctga tatcgcctgc gttgtcgttg acacactggc aggcgttatt tgccgatcca
    16081 cagttaccgc atgcattact ggcaacgctg gtgtcgacaa ccatcgcggc ggtcggggca
    16141 ttgttgattg ccctgctggt gattgtggcg ctatggcctg ggccgaaatg gcagcgtatg
    16201 tgcgcccgtc tgccgtggct gctcgccatt ccccatgtgg cttttgccac cagcgccctg
    16261 ctgctctttg ctgacggagg gctgctatat gactatttcc cgtatttcac tccgccaatg
    16321 gaccgatttg gcatcggtct gggcctcacc cttgcagtga aagaaagcgc atttctgctg
    16381 tggatcttag cggcagtatt gagcgaaaaa cggctgttgc agcaggtcat tgtgctggat
    16441 tcgctgggct acagccgctg gcaatgcctg aactggctgc tgttgccctc cgtcgcgcct
    16501 tcgctggcaa tggcgatgct ggcgattgtt gcctggtcgc tttcggtcgt ggatgtggcg
    16561 attattcttg ggccaggtaa tccaccgacg ctggcagtaa ttagctggca gtggttaact
    16621 cagggcgacg cggatcaaca aacgaaaggc gcactcgcca gtctgctgct gatgctgtta
    16681 ctcgccgcct acgttttgct gagctatctg ctatggcgca gctggcggcg cactattccc
    16741 cgcgtagatg gcattcgcaa gcctgccaca cctttattac caggcactac gctggcgagt
    16801 tttttaccct taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg
    16861 acgatcaata gtgaagcact catcaacagc ctgacaatgg ggctggtggc gacattcatc
    16921 gctttgctcc tgttactgct gtggctggaa tgggggccac agcgtcgcca gttgtggctc
    16981 tggttgccca ttctattgcc cgctctgcca ctggtggcgg gccagtacac gctggcgcta
    17041 tggctgaacc tggatggaag ctggacggcg gtggtctggg ggcatctgct gtgggtaatg
    17101 ccgtggatgc tgtttatcct gcaacccgcc tggcagcgca ttgattcacg gttaattttg
    17161 attgcgcaaa cactgggctg gtcgcgggcc aaaatcttct tttacgtgaa atgcccactc
    17221 atgttgcgcc ctgtgctgat tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat
    17281 atgccgacgc tgtggctggg cgcggggcgt tttccgacgc tcaccactga ggcggtggca
    17341 ttaagcagcg gcggcagcaa cggtattctc gccgcccagg ctttatggca actgttgtta
    17401 ccgcttatta tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa
    17461 ggactccgct aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc
    17521 tgacaaacgt taactttacg gtggataaag gtgacattgt cacgttaatg gggccgtctg
    17581 gctgtggaaa atccactctg ttttcatgga tgattggtgc actggctgga cagttttctt
    17641 gtacaggtga gctatggttc aatgagcaac ggattgacat gctacccacc gcacagcgtc
    17701 agattggcat tcttttccag gatgcactgt tatttgacca gttcagcgtc gggcaaaatt
    17761 tactgctggc gctaccgtcg acgctcaagg ggactgccag gcgcaatgcc gtgaaagacg
    17821 cgcttgatcg agcgggactg gctgaaactt atcaccagga ccctgcgaca ttatcaggcg
    17881 gtcagcgagc gcgggtggcc ctgctgcgcg cccttctcgc tcagccaaaa gcgttactcc
    17941 tggatgagcc attcagccgt cttgatgtgg ctctgcgcga taattttcgc cagtgggtgt
    18001 tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt aacgcacgat ctccaggatg
    18061 ttcctgctga tagttctgtt ctggatatgg cgcagtggtc agaaaattac aacaaactgc
    18121 gataacgcaa agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa actacaatgt
    18181 cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg
    18241 cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct tacacttgac cagcttcaac
    18301 aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt ttataacggc tggccacaaa
    18361 ccttaaatgg cccttctggt catgaacctg ccgccttaaa cctctctgcc agctggctcg
    18421 acaaaatgag caccgaacag ctctatgagt ggatcaagca acataatctg aaaaccgatg
    18481 ctccggtggc gctgtacggc aatgacaaag atgtcgacgc cgtcaaaacg cgtctgcaaa
    18541 aagcaggttt tacgcatatc tccattctga gtgacgcgct aagcgaacct tcccgtctac
    18601 aaaaactgcc gcactttgag cagctggttt atccgcaatg gctgcacgac ctgcaacaag
    18661 gtaaagaggt tacggcgaaa cctgccggtg actggaaagt cattgaagcg gcctggggcg
    18721 ctcctaagct ttaccttatc agccatattc ctggcgctga ctacatcgat accaacgaag
    18781 tggaaagcga accgctgtgg aacaaagttt ctgatgaaca actaaaagcg atgctggcaa
    18841 aacacggcat tcgccatgac accacggtca ttctgtacgg gcgtgacgta tacgctggag
    18901 cgcgtgtggc gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg ctggatggcg
    18961 gctggcaaac ctggtccgac gcgggactgc ctgttgagcg aggaacgcca ccgaaagtga
    19021 aagcggaacc ggatttcggc gtgaagatcc cggcacaacc gcagttgatg ctcgatatgg
    19081 aacaggcccg tggactgctg catcgccagg atgcatcgct ggtgagcatt cgttcgtggc
    19141 cagaatttat cggtacgacc agcggttaca gctatattaa accaaaaggt gaaatagccg
    19201 gagcacgttg gggacacgct ggtagcgact cgacgcatat ggaagatttt cataacccgg
    19261 atggcaccat gcgcagcgcc gatgatatta ccgctatgtg gaaagcgtgg aatatcaaac
    19321 cagagcagca agtttcattc tactgcggca ccggctggcg cgcgtccgaa acctttatgt
    19381 acgcacgcgc catgggttgg aagaatgttt ccgtgtatga cggcggctgg tacgaatgga
    19441 gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg cccggacagt agcaaataac
    19501 attgcaatta ctgacgctgg agcgacttca gcgtcagata cccactccac acccgcgtaa
    19561 aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac catgcaggga
    19621 ataaacatcc cagcacaaac agtaagatcg tttctgtgcc ttcggttaat ccccccagat
    19681 agtaaaacga cttatgtgca taaccggggt tatcaatctg atgttttgct gccagtgcag
    19741 caaaggcgag aaaactgctg cccgtgccga taaacgcaaa caacaaccag ccgcctgcca
    19801 gcgcattttg ctccggtgca gcaagaataa agccaaacgg caccagcgcg taaaagagaa
    19861 aatcgagaga aatatcaaga aagccgcccg cgtcggtaag ctctctgcgc cgcgccagcg
    19921 caccgtccag accatcaagc agcctgttca acaaaatgac gactagcgcc gccagatacc
    19981 agcccagcgc cagaaacggc agagccagca cgccgatggc aaatcccact aatgttaaac
    20041 catctggcgt aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa
    20101 tccggggatg aagatggcgg tctagcacag gcactcctta aatataaagc ctttctgatt
    20161 gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta atttatgaaa
    20221 atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact cgcgcaacgc
    20281 cccgcccaga gcgatcaggc gggattatgg gagtttgccg gtggtaaagt cgagctggat
    20341 gaaagccagc agcaggcact ggtgcgtgag ttaaacgaag aattgggcat cgaagcaact
    20401 gtgggtgagt atgttgccag ccatcagcga gaagtttcgg gccggattat ccatcttcat
    20461 gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca ggcgctggtc
    20521 tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat tccactatta
    20581 gaggcgttta tggctttacg cgccgccaga gcagcggatt agtgctaagg gttttgtcat
    20641 cccgctggca ttgcagcagt attccttcgg ctttaattac cgccccttca gaataatttt
    20701 gatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct gaactgaaca
    20761 cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc agtgcaatta
    20821 atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc ttaacgttat
    20881 tgtctctgct actgataacg gtagccgggt ggcaaaactt tagcgtctga gttatcgcat
    20941 ttggttatga gattactctc gttattaatt tgctttcctg ggtcattttt ttcttgctta
    21001 ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatcacata aacaacataa
    21061 gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat tctctggagt
    21121 cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc
    21181 gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat cgccagatgt
    21241 cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg
    21301 atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt gcagttcagc tctgccatcg
    21361 gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctctccatt ctcaaattcc
    21421 tcggcttcga acaaaccttc aaaaatgccc tgactaccct gccgatgggc ggtggtaaag
    21481 gcggtagcga ttttgacccg aaaggtaaaa gcgaaggcga agtgatgcgt ttctgccagg
    21541 cgctgatgac cgagctttat cgtcatctgg gcgcggatac cgacgttccg gcaggtgata
    21601 tcggcgttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag ctctccaaca
    21661 ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggtagtctt attcgcccgg
    21721 aagctaccgg ctacggtctg gtttatttca cagaagcaat gttaaaacgc cacggtatgg
    21781 gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag tacgctatcg
    21841 aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc ggcactgtag
    21901 ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc aaagccagcc
    21961 gcgatggtcg agtggcagat tacgccaaag aatttggtct ggtctatctc gaaggccaac
    22021 agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac gaactggatg
    22081 ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgctgaa ggggcaaata
    22141 tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg tttgcaccgg
    22201 gtaaagcggc taatgctggc ggtgtagcaa cgtcgggcct ggaaatggca caaaacgctg
    22261 cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc atgctggata
    22321 tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac gtgcagggcg
    22381 cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt gtgatttaag
    22441 ttgtaaatgc ctgatggcgc tacgcttatc aggcatacaa atgggcacaa ttcattgcag
    22501 ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca ggcgtttatg
    22561 agtatttaac ggatgatgct ccccagggaa catttcttat gggccaacgg catttcttac
    22621 tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc ttcaagttca gcatccgtta
    22681 actttctgcg atagcagcag atatgcctgt aaagaaatcc cattagacta tttttttgat
    22741 aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt atataatgcc
    22801 aggccagttc ttctccaatt gtcccgtttt gaaaagctgt gcttgatatc gagatcatcc
    22861 atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga ttattagcgc
    22921 aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg ataacctgct
    22981 ctggggcttc ctccatgttt gctttaaagg tattggctcc atggtcgcca gaaagaaaat
    23041 gctccattaa ggcacaataa ctttcgctat cttcgatacc ccattgatcc tctaaagact
    23101 cgcgtctttt acttatgata tcgatcgagt caaaaggaag cacatgatat tggaaggtat
    23161 ctttgccagg ttcaggcttt cgcggccaga actccagcgt ttcagaccat tgcttatgat
    23221 agaatcgata aggtgcgatc aattgtagcg cctgtaactt ctcgatactg agcggctcaa
    23281 tacctttagc ctgataataa tgcagttgtt ctttttttgc tttaaaaccg gcccgaacaa
    23341 taagccccat cataattaat agataaagaa aagagcatcc cgcagtaatc aggcctcttt
    23401 cattcaaacc gttggatgtt atcgctgcga atacaaacat tacagcgaca acacatgtta
    23461 aatacccccc cccacttaca aagcagcatg gccttatttt ctttaatcat ccgttcaaaa
    23521 ttactattaa atatttccca gccattaaaa gaatacttct cgctcccagg atggttttgt
    23581 aataaaactt ttttcatcac attccctgtt attacatact ttagaggcga taattttatc
    23641 catgcaaaaa aaatatccaa cgaattttct tgatccgctg gatatttcag aatatgactc
    23701 gaatagcacg aaagattcgc tcgcttacgc tatcgccccg cttccggctt catctgctgg
    23761 cggacttttt ttcgcactac gtttacgcgg tgcagccttt ttcttatcag cactgccacc
    23821 actgcccgga gccacaatgc cgcgaaactg ccgcaccggc gtacgtttgg cttgatcaat
    23881 aagctgatat agcgtcccca ccagcggctg cataaagtcc tgatagcgac actgcttttc
    23941 gctgatttgc gtcagcaccg attcccagtg cgcggtcatg tccggtcgcg tagccatttc
    24001 tggcagcgaa tggaataacg cttttccggc gtcggtggag tggatatagc gccctttttt
    24061 ggtgaggaaa ccacgcttga acaacagttc aataatcccg gcacgcgttg cttctgtccc
    24121 cagaccatcg gtcgcacgaa ggatcttttt cagatctttg tcctgcacaa agcgcgcaat
    24181 cccggtcatc gccgaaagca gtgttgcatc ggtaaaatgg cgcggcggct gggtttgccg
    24241 ctctaccact tcaccttttt cacacagcaa ctcatcgcct ttcgccacca caggcaatgg
    24301 cgtgccgtcg ttttcttcat cgcgctcttt gctgcctaac agcgcgcgcc aacctgcttc
    24361 agcaagaaaa cgcgctttag cgacaaattt gcctttggca atgtccagtt cgataacaca
    24421 cttgcggaac actgcatccg ggcagaactg catcagatac tgacgggcaa tcaggttata
    24481 gaccttcgct tcgttctccg tcaggttgat cgcagaactc cttgcggtcg gaatgatggc
    24541 gtggtgcgca tcgacctttt tgtcatccca acagcggttg cgtatatctg gatctaccac
    24601 tggctgcggc aacagatctg gtgcatgaac gctgatggca ttcatcaccg cgtggcgtcc
    24661 ggcaaaatgt tcttctggca aatagcgaca atcggaacgc ggataagtga ttaacttgtg
    24721 tgtttcgtac agcttctggc agatatcaag cacgttctgc gcgctcagac caaagcgttt
    24781 tgccgcttca atctgcaacg ccgaaagcga aaacggcagc ggcgcggatt ctgattcccg
    24841 tttatcgtta tagctggtga caatagccgg ttgaccacta atgcggttaa ccacatgctc
    24901 cgccagtgga cgatgtaaca agcgcccttc ttcatcctgg tacggttcac acgcttcgct
    24961 cggttgccag atagcggtaa accgctcatc ggcaggtgtc acgatatgcg ctttgacttc
    25021 aaagaagtct ttcgccacga agttttcaat ctcttcatcg cggcgcacca ccagcccgag
    25081 cacgggtgtc tgcacgcgtc ccacggaaag tacgccctga taaccggcat tgcgaccgag
    25141 aatggtatac gcacgggtca tattgatgcc gtacagccag tcggcacgcg ctcgcgccag
    25201 cgcagaaacg cacaatggca caaactcact gttggaacga agacggtcga tcgcccgctc
    25261 aaccgcctgc gggttcaggt cgtttatcaa gcaacgctgc acctgctggc gcttttccgg
    25321 tgccagttgc agatagtcca gcacttcatc caccagcaat tgcccttcac gatccgggtc
    25381 cccggcgtga acgatttcgc tggcttcatg caggaagcgt ttgatgacgt taagttgttt
    25441 ggtcacggag ggtcggggct gtaattgcca cttttccggg acaatcggca aatccgcaag
    25501 attccagcgc gcatagcggc tgtcgtaggc gtctggctgc gcctgctcaa gcaggtgacc
    25561 gatacaccag gtcaccacct gaccattacc gcactcgata aagccatcgc ctttacggtg
    25621 cggtttgggc aggacatcag caatggcgcg cgccagactc ggtttttcgg caataaacaa
    25681 ccgcattgaa ttaacggatc tcaaccatgg cacgaccgcc gcgcgctggc accagttcgc
    25741 caatcgccgt cagttcaatg ccaaactcgg cggctgtagt tttaacctca ttttctgctt
    25801 ccggcatgac cgccagcagc aaaccgccag aagtttgcgg atcgcacagc agatcgcgca
    25861 cttcacgcgg catttcaccc atcagatgac cgtagctggc aaagttacgt tcggtgccgc
    25921 caggcactgc gcccaactta atgtactctt caacccccgg caattttggg atcgcgtcat
    25981 agtcaacgcg cgcctgcaca cctgcgccct gacacatttc gctcaaatgg cccagcagac
    26041 caaaaccagt aacgtcggtc atcgctttta cgccttcgat gttggcaaag gacgcacctg
    26101 cgatgttcat ccggcacatc acttccgtcg ccagtccctg atgttctggt ttcaacagtg
    26161 attttttctc agccgtggta agaacgccga tccccagcgg tttcgtcagg aacagtttgc
    26221 atccggcttg tgcggtactg tttttcttca cccgctcggt cggtacgatc cccgttaccg
    26281 ccagaccaaa aatcggctcc ggcgcatcga tggagtgacc cccagccagc gcaatacccg
    26341 cctgacggca tgcatagcgt ccaccttcgg ttacttcgcg ggcaatttct ggggaaagtt
    26401 tgttaatcgg ccagccgagg atcgcaatcg ccataatcgg tttgcccccc atcgcgaaga
    26461 tatcgctgat ggcgttagtc gccgcaatgc ggccaaaatc gaaaggatta tcaacgatcg
    26521 gcataaagaa gtcggtggta ctgataacgc tggtgccatt gcccagatcg tacaccgccg
    26581 catcgtcgcg ggtctcatta cccacaagca aattcggatc aacaaacttc gcctgctcac
    26641 tgtgcaggat ggtttccaac acttttgggg aaattttaca gccgcaacca gctccgtggc
    26701 tgtattgggt caaacgaatc gagttctcgc tcatggacat ctcctgtcaa tgcaatccgg
    26761 gtatggtaac cctcattccg tgaagtgata agtgagagtg tctgaattcc tgcgcctttg
    26821 ctcacaatcc agacagtttc gcgacaatta tcagaaataa gttacaaacg gcgtcgggtc
    26881 cgggacgtta atcgacgtag atgctttcaa ctgcggcgtg ccgaggtaga gaaaaccgac
    26941 aattttatcc tgctcacggc aaccgaatgc ttcacgcact accggacttt cagttaatgc
    27001 gccactgcgc cagatgccgc caaatccttg ggcaacggct gccatttgca tcgccatgac
    27061 cgcgcatccg gcagacattt cctgttccca tcgcgggact ttatgatttt cttcgcattt
    27121 cgccaccacc gtgatgatga gcggtgcgcg gaacggcgca ttacgggctt tgtcgatagc
    27181 tttgtcatca ctaccggcag caatcgcccc ctgttccagt acggcgctga aacgctcgcg
    27241 cccttcccct tcaatcacaa aaaaatgcca cggttgcatg gacttatggt ccggcgcacg
    27301 catacccgca cgcaggatgt tttgcagttg ttcacccgtt ggcgcgggtt cagccaggcg
    27361 ggaggcgcta cggcgattga tcaatagttc gagtgcatcc atttgattaa ctcctgtcgt
    27421 gatatttatt cacaaaatta acacgagagt ggattttgtt acagcacagt ccgcaattcc
    27481 tgctgacaag tgccggttgg gtcattacga taaccacatc tattgcgcct gtgacaggtg
    27541 tgaccttaag ttgggagaat acatgcgaac cctttggcga tttattgccg gattttttaa
    27601 atggacgtgg cgtctgctga atttcgtccg tgaaatggta cttaacctgt tctttatttt
    27661 cctcgtactg gttggtgtgg ggatctggat gcaggtcagt ggtggtgatt cgaaagaaac
    27721 ggccagtcgt ggcgcactgc tgctggacat ttctggtgtg atcgtcgata aacccgacag
    27781 ttctcagcgg tttagtaaat taagccgcca gctgcttggt gccagttccg atcgtctgca
    27841 ggaaaactca ctgtttgata tcgtcaacac cattcgccag gcgaaggacg accgcaatat
    27901 caccggtatc gtgatggatc tgaaaaactt cgcaggcggc gaccagccgt ctatgcagta
    27961 catcggcaaa gccctgaaag agtttcgtga cagcgggaaa ccggtttatg ccgttggcga
    28021 gaactacagc caggggcaat attatctcgc cagtttcgcc aataaaattt ggctgtcacc
    28081 gcaaggcgtg gttgatctgc acggttttgc taccaacggt ctgtactaca aatcgttgct
    28141 ggataagctg aaagtttcca cccatgtgtt ccgcgtgggt acgtataaat ctgccgttga
    28201 accgtttatt cgtgatgata tgtcaccggc agcccgcgaa gctgacagcc gctggattgg
    28261 tgagctgtgg caaaactatc tgaatactgt tgccgctaac cggcagatcc ctgctcagca
    28321 ggtattccct ggcgcgcaag ggttgcttga gggtttaacc aaaaccggtg gcgataccgc
    28381 gaaatatgca ctggaaaaca agctggtcga tgcactggca tcgagtgccg aaatcgaaaa
    28441 agctctgacc aaagagttcg gctggagtaa gactgataaa aattatcgcg ccatcagtta
    28501 ttacgattac gcattgaaaa cgccggcaga taccggtgac agcatcggtg tcgtcttcgc
    28561 taatggcgca attatggatg gcgaggaaac tcaggggaat gttggcggtg ataccactgc
    28621 ggcacaaatc cgcgacgctc gccttgaccc gaaagtgaaa gcgattgtcc tgcgtgttaa
    28681 tagcccaggc ggcagcgtta ccgcgtctga agtgattcgc gctgaactgg cagcagcccg
    28741 ggcagcgggt aagcctgtgg ttgtatcgat gggcggcatg gcggcatctg gtggttactg
    28801 gatttccacg ccagctaatt acattgtggc taaccccagc accctgaccg gttctatcgg
    28861 tatcttcggc gtgatcacca ccgtagaaaa tagtctggat tcgattggtg ttcatactga
    28921 tggtgtctca acttcaccgc tggcggatgt ttctatcacc agggcactgc cgccggaagc
    28981 gcagcagatg atgcaattaa gcattgagaa tggctataaa cgctttatca cgctggttgc
    29041 tgatgcgcgt cattcgacgc cggagcaaat tgataaaatc gcccagggcc acgtctggac
    29101 cggtcaggat gcaaaagcta acgggctggt cgatagtctc ggggatttcg atgatgcggt
    29161 tgccaaagca gcagagctgg caaaagtgaa acagtggcat ctggaatact acgttgatga
    29221 accgaccttc ttcgacaaag tgatggacaa catgtctggt tctgtccggg caatgttgcc
    29281 agatgcgttc caggccatgt tacctgcacc gcttgcctcg gtagcctcta ccgttaaaag
    29341 tgaaagcgac aagctggccg cgtttaacga cccacaaaac cgttatgcgt tttgcctgac
    29401 ctgcgccaac gtgcgttaag tcttgtactg agtggccgac agatcgtcgg ccacattatt
    29461 ttttacctcg acgaatcctc ttcccgctgt ctcgacccat atttccttat ccgcatagta
    29521 tcaggtgcgc tcctcctgcc tcacgtatat acttttgctc tttcgatatc attcatatca
    29581 atatcatgca aaagaaatca atttacgtcg cctacacggg cgggaccatc gggatgcagc
    29641 gttccgagca gggttatatt ccggtgtcag gtcatctaca acgccaactg gcgctgatgc
    29701 cggaattcca tcgcccggag atgccagatt tcaccattca tgaatatacg ccgttgatgg
    29761 attcttctga tatgacgccg gaagactggc agcatattgc tgaagatatt aaagcgcact
    29821 atgacgacta tgatggtttt gtcattctac acggtaccga cacgatggcg tataccgcct
    29881 ctgcgctgtc gttcatgctc gagaatctcg gtaaaccggt cattgtgaca gggtcacaaa
    29941 tcccgctggc tgagttacgc tctgacggac aaattaatct gctgaatgcg ttgtacgttg
    30001 cggcgaatta tccgatcaac gaagtaacgc tctttttcaa taaccgattg tatcgcggca
    30061 accgcactac caaagcccat gccgatggtt ttgatgcgtt tgcctctcca aaccttcctc
    30121 cgttactgga agctggtatc catattcgtc gtttgaatac gccacccgcc ccgcacggtg
    30181 aaggggaatt aatcgttcat ccaatcacgc cacaaccaat tggcgtagtg acgatttatc
    30241 cggggatttc tgctgatgtc gtgcgcaatt ttctgcgcca accggtgaaa gcattgattc
    30301 tgcgctccta tggcgtgggt aatgcgccac aaaacaaagc cttcctgcag gaattacaag
    30361 aagccagcga tcgcggtatt gtggtggtta acctgacaca atgtatgtcc ggtaaagtga
    30421 acatgggtgg ttatgccacc ggtaacgccc tcgcccatgc cggcgtaatt ggcggtgcag
    30481 atatgactgt agaagctacg ctaaccaaac tgcattacct gctgagccag gaactggata
    30541 ctgaaaccat ttgcaaggcc atgagccaaa acctgcgcgg cgaactgacg ccggatgatt
    30601 aaggagactg caatgccccc tcgcgccctg ttactggtcg atttacaaaa tgatttctgt
    30661 gctggtggcg cgctcgccgt gccggaaggt gacagtacgg tggatgtcgc taaccgcctt
    30721 attgactggt gccagtcgcg cggtgaagcg gttatcgcca gtcaggactg gcacccggcg
    30781 aatcacggca gttttgccag tcagcactgt gtagagcctt atatgccagg tcaactcgac
    30841 ggtttgccac aaaccttctg gccagatcac tgtgtgcaga acagtgaagg cgcacaatta
    30901 catccgttac tgaaccaaaa agcgatcgca gcggtgttcc ataaaggcga aaatccttta
    30961 gttgacagtt acagtgcctt ttttgataac ggccgtcggc agaaaacctc tctcgatgac
    31021 tggttacgcg atcatgaaat cgatgaattg atcgttatgg gcctggctac tgactattgc
    31081 gtgaagttta ccgtgctgga cgcgttacag ttaggttata aagtaaacgt gattaccgat
    31141 ggttgtcgtg gcgtgaatat ccagccccag gacagtgcgc acgcgtttat ggagatgtca
    31201 gcagctgggg caacgctata tacgctggca gactgggaag agacgcaggg gtaatattag
    31261 ttgaattcat ctctggccta catttcctca atgacattgt aggccagata atacttgtca
    31321 gtgttgcatt taacctactt gcagctaaaa cacgacgtcc taacaagtat atttgccctc
    31381 ccttaataac aggcttaaat attagataac tcattcactt aaacatagca ataggctttg
    31441 ctgagatacc aaaatcttct tttaactgct gtttgctctt catgaccatc tgaccatttg
    31501 tgtcaattgt catatgctgt gcttcggtat tatggcgggc ttgccacagc atcaccagtt
    31561 gcaggcaatt ctctttttgt tcctcggtca acgcaacgcc atcaggccat ttccccagtt
    31621 caacggcggt cgacaaacgc tggtatacct caggcgtcat gctgttgata atgtcatcaa
    31681 gattcataat ttttccgctc ctgtggaata agttgctgaa tcgttttttc aaccgttgat
    31741 ttcttcgccg ttttcgccat cggtaaagcg taaagaggca gagttaacac aataacgttc
    31801 gcccgttggc tgcggcccgt cagggaagac atgccccaga tgggcatcac agttaccgca
    31861 acgaatttct atgcgctgca ttccatgtga caagtctttg atataacgaa tggattcttc
    31921 actcaccggt tcgtagaaac tgggccaacc acagccggaa tcatacttgg tttgggaatg
    31981 aaacagcggg gcatcgcaga tcaaacagtg atatacgccg tcacgcttgt tatgcagtaa
    32041 acgacccgta aatggcggct ctgtcccatg attctgcgtc acgtaaaact gcatctcgga
    32101 caaatttttt ttcagttctt ctgccgaagg tttattagcc atttgctcac atctcacttt
    32161 aatcgtgctc acattacgtg actgattcta acaaaacatt aacaccaact ggcaaaattt
    32221 tgtcctaaac ttgatctcga cgaaatggct gcacctaaat cgtgatgaaa atcacatttt
    32281 tatcgtaatt gccctttaaa attcggggcg ccgaccccat gtggtctcaa gcccaaagga
    32341 agagtgaggc gagtcagtcg cgtaatgctt aggcacagga ttgatttgtc gcaatgattg
    32401 acacgattcc gcttgacgct gcgtaaggtt tttgtaattt tacaggcaac cttttattca
    32461 ctaacaaata gctggtggaa tatatgacta tcaaagtagg tatcaacggt tttggccgta
    32521 tcggtcgcat tgttttccgt gctgctcaga aacgttctga catcgagatc gttgcaatca
    32581 acgacctgtt agacgctgat tacatggcat acatgctgaa atatgactcc actcacggcc
    32641 gtttcgacgg taccgttgaa gtgaaagacg gtcatctgat cgttaacggt aaaaaaatcc
    32701 gtgttaccgc tgaacgtgat ccggctaacc tgaaatggga cgaagttggt gttgacgttg
    32761 tcgctgaagc aactggtctg ttcctgactg acgaaactgc tcgtaaacac atcaccgctg
    32821 gtgcgaagaa agtggttatg actggtccgt ctaaagacaa cactccgatg ttcgttaaag
    32881 gcgctaactt cgacaaatat gctggccagg acatcgtttc caacgcttcc tgcaccacca
    32941 actgcctggc tccgctggct aaagttatca acgataactt cggcatcatc gaaggtctga
    33001 tgaccaccgt tcacgctact accgctactc agaaaaccgt tgatggcccg tctcacaaag
    33061 actggcgcgg cggccgcggc gcttcccaga acatcatccc gtcctctacc ggtgctgcta
    33121 aagctgtagg taaagtactg ccagaactga atggcaaact gactggtatg gcgttccgcg
    33181 ttccgacccc gaacgtatcc gtagttgacc tgaccgttcg tctggaaaaa gctgcaactt
    33241 acgagcagat caaagctgcc gttaaagctg ctgctgaagg cgaaatgaaa ggcgttctgg
    33301 gctacaccga agatgacgta gtatctaccg atttcaacgg cgaagtttgc acttccgtgt
    33361 tcgatgctaa agctggtatc gctctgaacg acaacttcgt gaaactggta tcctggtacg
    33421 acaacgaaac cggttactcc aacaaagttc tggacctgat cgctcacatc tccaaataag
    33481 ttgagatgac actgtgatct aaaaagagcg acttcggtcg ctcttttttt tacctgataa
    33541 aatgaagtta aaggactgcg tcatgattaa gaaaattttt gcccttccgg tcatcgaaca
    33601 aatctcccct gtcctctccc gtcgtaaact ggatgaactg gacctcattg tggtcgatca
    33661 tccccaggta aaagcctctt ttgcattaca gggcgcacac cttctctcgt ggaaacctgc
    33721 gggtgaagaa gaagttctgt ggttgagcaa caacacaccg ttcaaaaatg gcgtcgctat
    33781 tcgcggtggc gtaccggttt gctggccgtg gtttggtccg gcggcacaac aaggtctgcc
    33841 tgcgcacggt tttgcccgca acctgccgtg gacgctgaaa tcacatcgtg aagatgctga
    33901 tggcgtagcg ctgacttttg aattgacgca aagcgaagag acgaaaaaat tctggccgca
    33961 cgactttacg ctgttagcgc atttccgcgt gggtaaaacc tgtgaaatcg atcttgaatc
    34021 acatggcgaa tttgaaacca cctctgccct gcatacctac tttaacgtgg gtgatatcgc
    34081 taaggtaagc gtcagtgggc tgggcgatcg cttcattgat aaagtaaatg acgcgaaaga
    34141 agatgtactg accgacggta ttcagacctt ccctgaccgt accgatcgcg tgtatctgaa
    34201 tccgcaagat tgcagcgtga ttaatgatga agcgctgaat cgtattatcg ccgtaggcca
    34261 ccagcatcat ctgaacgttg tcggctggaa cccgggaccg gcgctttcag ttagcatggg
    34321 cgacatgccg gatgatggct acaaaacatt tgtttgtgta gaaacggctt acgcttcaga
    34381 aacgcaaaaa gtgaccaaag agaaacctgc acatctggcg caatccattc gcgttgcgaa
    34441 acgttaattt gcgttaatgt tgtgtgccgg gtgcgatgca tccggcacgt atcaacatca
    34501 caccatatcc agcgcagttt ttccttttgg tgccggatac gctttatcca gcatgattaa
    34561 ttccgctgac gaaagttcga cctcaagcac agccgcattc tgttggacat gggcaatcgt
    34621 ggccgctttt ggaatcgcca tcacaccctg atggctgatt acccacgcca acaatacttg
    34681 tgccgcgctg atattgtgag catgtgcaat ttcgttgact accgcgtttt ttaacagtcc
    34741 attgcgcaac cgcccggcct gggctaatgg actgtaagcc attaccggca tctgctgttg
    34801 ctggcaccag gggagtagat cgtactcaat ccctcgtgaa ccgagatggt aaagcacctg
    34861 attagtggca cactgatttc cccccggcag ctgccagagt tcctgcatat cagcataatc
    34921 aaggttagaa acgccccagc ggcggatttt tccctgggcg atcaattttt ccatcgctgc
    34981 cacagtctct tcaaaagcga aactgccaga ccagtgtaat aagtagagat cgagataatc
    35041 agtattgaga cggcgtaaac tggcttcgca tgcatttatc gctttttgcc cgccagcatt
    35101 ccacggatag actttagaga cgagaaagac gttatcgcgc agatcggtta atgcttcccc
    35161 aaccaccttt tcggcaccgc catcggcata catttcggcg gtatcaatga gggttaaacc
    35221 gagctcaatg cccgcgcgta gtgcagcaac ttctgttttg cgctgactgg catcttcgcc
    35281 catataccat gttccctgcc ctatggctgg cagtgagaca tcgccactaa attgaatcat
    35341 tttttgttgc attgtttcct cccaggtatt gcaccaccgt aatgcaaaac agggcgtgac
    35401 gccctgtttt tatgcacaaa atgccctgga aagatgcatt atcagaattt gtaggtgatc
    35461 ccggtagaaa tcaggccagt ccaggattta tccaccatcg ggctgtcagt aacttcatca
    35521 gacagacggg tatagcgcgc ggtaccgtaa acactccagt cgccgaggaa gttgtagctg
    35581 gcgctcagct ccaggtaagg gctccagctg tcgttcgggt tatagccacg cagaccgctg
    35641 cgagcggact ctttgcgcga tacgccataa tagtattcgt tctggttttc gctgttccac
    35701 tgcacaccaa tacccggagt cacggtcagg ccaccgttgg tgtaacgata caaccaggcc
    35761 atatcccaga caatgccgtt gctgttatcc agggtatcgc cagccagggt ggtacgcagg
    35821 taaccgtact gggtaaagtg agcataagac agaccagcca tcatggtgct cttacggtca
    35881 tccaggtgac gcatttggtg atcgccactg tctttcgctt tgaagtaaag cggcgaccag
    35941 taagcggtaa ttgaaagttt atccgttgcg tcattccaca ggtagtaacc accacctaag
    36001 ccacggaacc agaagttatc gccttcatag ttgattaccg gtactgggta aacatcggta
    36061 tcgtaatctt tatatgggtg ctcaacgaca cctacgcctg cgcccaggga aaatttacct
    36121 tcagcgtgcg ctacgcctgc agacgttgcg ataagcactc caagtgccag aagtttgagt
    36181 ttggtcacaa ttaatcattc cttaaacaaa tgtttagcgg gcgacaaagt ttacccgtca
    36241 atacatcgaa acccaacctt tttacgtttt cattttttaa agtaactgtt taattttcct
    36301 gacgcggatg acaccgcgct tacagccaaa tgaatttagc gttactggct accctggtct
    36361 ttacattaat tatgcaaaat ttatggatga gttgttgata tgccattgaa attaagaaag
    36421 ccgtgcaggc aagttttcca tttgccatct acgcttaatt ttgaaggtgt atcaccgggc
    36481 acgttgttct catcgtcgat aaaatggcat gagagttgct gtgttttagc aagagacgtc
    36541 gttcagttta cctcttccgg gagcctctac tattcatatg aacggctctt aacctgtgct
    36601 aaaaaacgaa aggacggcat accatgaata tattcgatca ctatcgccag cgatatgaag
    36661 ctgccaagga cgaagagttc acactgcagg agtttcttac cacttgtcgg caagatcgca
    36721 gtgcttatgc caacgcggct gagcggctat tgatggctat cggtgagcct gtcatggtcg
    36781 atacagccca ggaacccaga ctttctcgac tcttttctaa ccgggtcatt gcacgttatc
    36841 cggcgtttga agagttttac ggcatggaag acgcgattga acagattgtc tcttatctga
    36901 aacacgcggc tcaggggctg gaagagaaga aacaaatcct gtatctgctg gggcctgtgg
    36961 gtgggggtaa atcatcgctt gctgagcgac tgaaatcatt aatgcagctc gtaccgattt
    37021 atgtattgag cgccaacggt gagcgtagcc cggtcaacga tcatccgttc tgtcttttca
    37081 atccgcagga agatgctcag attctggaaa aagagtatgg cattcctcgc cgttatctcg
    37141 gcaccatcat gtcgccatgg gcggcaaaac gcctgcatga atttggtggc gatatcacta
    37201 agttccgggt agtgaaggtc tggccgtcaa ttctgcaaca aattgctatc gccaaaacgg
    37261 aacccggtga tgagaacaac caggacatct ccgcgctggt tgggaaagtc gatattcgta
    37321 aactcgaaca ctacgcgcag aatgacccgg acgcctacgg ctattccggt gcgctgtgcc
    37381 gcgccaacca agggatcatg gagttcgttg agatgtttaa agcaccgata aaagtgctgc
    37441 atccgctgtt aaccgccact caggaaggta actacaacgg gacggaaggt atctccgccc
    37501 tgccgttcaa cgggattatc ctcgcccact cgaacgaatc cgaatgggtc actttccgta
    37561 ataacaaaaa caacgaagcc tttctcgacc gtgtttacat cgtgaaggtg ccgtattgct
    37621 tgcgcatttc cgaagagatc aaaatctacg agaaattgct taatcacagt gaattgactc
    37681 acgccccatg cgcccctggc acgctcgaaa cactgtcacg tttttccatt ctttcgcgcc
    37741 tgaaagagcc agaaaactcc agcatttatt caaagatgcg ggtttatgat ggcgaaagtc
    37801 tgaaagacac cgatcccaaa gccaagtcgt atcaggaata tcgtgactac gccggtgtcg
    37861 atgaagggat gaacggtctg tcgacgcgtt ttgcgtttaa gatcctctcc cgcgtgttca
    37921 acttcgatca tgtagaagtg gcagcaaacc cggtccatct gttctacgtc ctggaacagc
    37981 agatcgagcg cgagcagttc ccacaagagc aggcagaacg ctatctggag ttcctgaaag
    38041 gttatctgat cccgaaatat gccgagttta tcggcaaaga gatccagacg gcctaccttg
    38101 aatcctattc cgaatatggg caaaacattt tcgaccgtta tgttacctac gcggatttct
    38161 ggattcagga tcaggagtat cgcgatccgg ataccgggca gctgtttgac cgcgagtctc
    38221 ttaacgccga gctggagaaa atcgagaaac cggcggggat cagtaatcca aaagatttcc
    38281 gcaacgagat tgttaacttc gtactgcgcg ccagagcgaa taacagcgga cgaaatccga
    38341 actggagcag ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc tccaataccg
    38401 aggagctgtt gccggttatc tcgtttaacg ccaaaacgtc aaccgacgag cagaagaaac
    38461 acgacgactt tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag gtgcgtttac
    38521 tgtgcgaatg gtatttgcgc gtacgtaaat cgtcttaaca accctggccc ggtacacatg
    38581 ctaccgggcc tacaacgaca gcgaaccgtg ggccagagaa atggcaacac aggcgtagca
    38641 tacagttggc aaatgtagta cggggggcat atgacctggt ttattgaccg gcgtctgaac
    38701 ggcaaaaaca aaagcatggt gaatcgccag cgttttttac gccgttataa agcgcaaatt
    38761 aaacagtcga tctccgaggc cattaataag cgttcggtga ctgacgtcga cagcggcgaa
    38821 tccgtatcca ttcccacgga agatattagc gaaccgatgt ttcatcaggg gcgtggcggt
    38881 ctgcgccacc gcgtgcatcc gggcaatgac catttcgtcc agaacgaccg aattgaacgt
    38941 ccccagggtg gcggcggagg ttccggcagt ggacagggcc aggccagcca ggatggtgaa
    39001 ggtcaggatg aatttgtctt tcagatttcg aaagatgagt atcttgatct gctctttgaa
    39061 gatttggctt taccgaatct gaaacaaaac caacaacgcc agctgaccga atataaaacg
    39121 catcgggcgg gttataccgc taacggcgtt ccggccaata tcagcgttgt acgttccttg
    39181 cagaactcac tggcgcgtcg cacagctatg acggcgggca agcgacggga acttcatgcg
    39241 ctggaagaga atttggccat catcagcaac agtgaacctg cgcaactgct ggaagaggaa
    39301 cgtctgcgca aagaaattgc agaattacgt gccaaaattg aacgcgtccc tttcattgac
    39361 accttcgatt tacgttacaa gaactacgag aagcggcccg atccctccag ccaggcagtg
    39421 atgttttgcc tgatggacgt ttccggttca atggatcaat ccactaaaga tatggctaag
    39481 cgtttttata ttctgctgta tctgttcctc agcagaacgt ataagaacgt ggaagtcgta
    39541 tacatccgcc atcataccca ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag
    39601 gaaacaggcg gcaccattgt ttccagcgcc ctgaaactga tggatgaggt agtgaaagag
    39661 cgttataacc cggcacagtg gaatatttac gctgcacaag catcggacgg cgataactgg
    39721 gccgatgact ctccgctttg ccatgaaatc ctggcgaaaa aattattacc tgttgttcgt
    39781 tattacagct atatcgaaat tacccgtcgt gcacatcaga cattgtggcg agaatatgag
    39841 catctgcaat ctactttcga caactttgcg atgcagcaca tccgcgacca ggatgatatt
    39901 tatccggtat tccgtgaact gtttcataaa caaaatgcaa cagctaaaga ctaaaactat
    39961 cagccgggtc attatcgcct ggctgatttt tagcttactg taaattatct catttattac
    40021 atacattagc ttacaatcgc tttaaatatg acagcataac ctttacataa tttagttcca
    40081 gaaaacaatc attcggaaaa atgattcagt caacacgtat ttccatgggg ttattcttta
    40141 aatatttttt atcgttaacg aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa
    40201 taaaatccag cactcacatt gctctccttt ttatggtttc tatgggtaca caaaaattaa
    40261 aagctcaaag cttttttatt ttcagtttat tgctgac
//
LOCUS       DAZGCS010000038        39603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGCS010000038 DAZGCS010000000
VERSION     DAZGCS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.038"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..965)
                     /gene="rfaP"
                     /locus_tag="ACHK01_002042"
     CDS             complement(168..965)
                     /gene="rfaP"
                     /locus_tag="ACHK01_002042"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO8096997.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(958..2082)
                     /gene="waaG"
                     /locus_tag="ACHK01_002043"
     CDS             complement(958..2082)
                     /gene="waaG"
                     /locus_tag="ACHK01_002043"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8096998.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2079..3137)
                     /gene="rfaQ"
                     /locus_tag="ACHK01_002044"
     CDS             complement(2079..3137)
                     /gene="rfaQ"
                     /locus_tag="ACHK01_002044"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO8096999.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            3550..4827
                     /gene="waaA"
                     /locus_tag="ACHK01_002045"
     CDS             3550..4827
                     /gene="waaA"
                     /locus_tag="ACHK01_002045"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO8097000.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4835..5314
                     /gene="coaD"
                     /locus_tag="ACHK01_002046"
     CDS             4835..5314
                     /gene="coaD"
                     /locus_tag="ACHK01_002046"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO8097001.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5353..6162)
                     /gene="mutM"
                     /locus_tag="ACHK01_002047"
     CDS             complement(5353..6162)
                     /gene="mutM"
                     /locus_tag="ACHK01_002047"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO8097002.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6260..6427)
                     /gene="rpmG"
                     /locus_tag="ACHK01_002048"
     CDS             complement(6260..6427)
                     /gene="rpmG"
                     /locus_tag="ACHK01_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO8097003.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6448..6684)
                     /gene="rpmB"
                     /locus_tag="ACHK01_002049"
     CDS             complement(6448..6684)
                     /gene="rpmB"
                     /locus_tag="ACHK01_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO8097004.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(6901..7569)
                     /gene="radC"
                     /locus_tag="ACHK01_002050"
     CDS             complement(6901..7569)
                     /gene="radC"
                     /locus_tag="ACHK01_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8097005.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7741..8961
                     /gene="coaBC"
                     /locus_tag="ACHK01_002051"
     CDS             7741..8961
                     /gene="coaBC"
                     /locus_tag="ACHK01_002051"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO8097006.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8939..9397
                     /gene="dut"
                     /locus_tag="ACHK01_002052"
     CDS             8939..9397
                     /gene="dut"
                     /locus_tag="ACHK01_002052"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO8097007.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRVCLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            9504..10100
                     /gene="slmA"
                     /locus_tag="ACHK01_002053"
     CDS             9504..10100
                     /gene="slmA"
                     /locus_tag="ACHK01_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO8097008.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10137..10778)
                     /gene="pyrE"
                     /locus_tag="ACHK01_002054"
     CDS             complement(10137..10778)
                     /gene="pyrE"
                     /locus_tag="ACHK01_002054"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO8097009.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(10844..11560)
                     /gene="rph"
                     /locus_tag="ACHK01_002055"
     CDS             complement(10844..11560)
                     /gene="rph"
                     /locus_tag="ACHK01_002055"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO8097010.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11687..12550
                     /gene="yicC"
                     /locus_tag="ACHK01_002056"
     CDS             11687..12550
                     /gene="yicC"
                     /locus_tag="ACHK01_002056"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO8097011.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            complement(12567..12707)
                     /locus_tag="ACHK01_002057"
     CDS             complement(12567..12707)
                     /locus_tag="ACHK01_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097012.1"
                     /translation="MSISRSQCAGQKNIAKSGAGTQNRIEVAGKKNAALFEWAAFVKA
                     EK"
     gene            12771..13595
                     /gene="dinD"
                     /locus_tag="ACHK01_002058"
                     /pseudo
     CDS             12771..13595
                     /gene="dinD"
                     /locus_tag="ACHK01_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            13887..14504
                     /gene="yicG"
                     /locus_tag="ACHK01_002059"
     CDS             13887..14504
                     /gene="yicG"
                     /locus_tag="ACHK01_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO8097013.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(14501..16132)
                     /gene="ligB"
                     /locus_tag="ACHK01_002060"
     CDS             complement(14501..16132)
                     /gene="ligB"
                     /locus_tag="ACHK01_002060"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO8097014.1"
                     /translation="MWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVY
                     DQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDL
                     WVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTL
                     QGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDR
                     LKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWL
                     PGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSV
                     RRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVC
                     QEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAK
                     SKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPG
                     TGSGRARQVTEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16044..16301)
                     /locus_tag="ACHK01_002061"
     CDS             complement(16044..16301)
                     /locus_tag="ACHK01_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097015.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFTPATANKTVGR"
     gene            16441..17064
                     /gene="gmk"
                     /locus_tag="ACHK01_002062"
     CDS             16441..17064
                     /gene="gmk"
                     /locus_tag="ACHK01_002062"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO8097016.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17119..17394
                     /gene="rpoZ"
                     /locus_tag="ACHK01_002063"
     CDS             17119..17394
                     /gene="rpoZ"
                     /locus_tag="ACHK01_002063"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO8097017.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            17413..19521
                     /gene="spoT"
                     /locus_tag="ACHK01_002064"
     CDS             17413..19521
                     /gene="spoT"
                     /locus_tag="ACHK01_002064"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO8097018.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            19528..20217
                     /gene="trmH"
                     /locus_tag="ACHK01_002065"
     CDS             19528..20217
                     /gene="trmH"
                     /locus_tag="ACHK01_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO8097019.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            20223..22304
                     /gene="recG"
                     /locus_tag="ACHK01_002066"
     CDS             20223..22304
                     /gene="recG"
                     /locus_tag="ACHK01_002066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO8097020.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(22289..23155)
                     /locus_tag="ACHK01_002067"
     CDS             complement(22289..23155)
                     /locus_tag="ACHK01_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097021.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(23158..24363)
                     /gene="gltS"
                     /locus_tag="ACHK01_002068"
     CDS             complement(23158..24363)
                     /gene="gltS"
                     /locus_tag="ACHK01_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO8097022.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24643..26034
                     /gene="xanP"
                     /locus_tag="ACHK01_002069"
     CDS             24643..26034
                     /gene="xanP"
                     /locus_tag="ACHK01_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO8097023.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26155..27864
                     /gene="yicH"
                     /locus_tag="ACHK01_002070"
     CDS             26155..27864
                     /gene="yicH"
                     /locus_tag="ACHK01_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8097024.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(27917..30235)
                     /gene="yicI"
                     /locus_tag="ACHK01_002071"
     CDS             complement(27917..30235)
                     /gene="yicI"
                     /locus_tag="ACHK01_002071"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO8097025.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRQDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(30245..31627)
                     /gene="yicJ"
                     /locus_tag="ACHK01_002072"
     CDS             complement(30245..31627)
                     /gene="yicJ"
                     /locus_tag="ACHK01_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO8097026.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            31920..32014
                     /locus_tag="ACHK01_002073"
     tRNA            31920..32014
                     /locus_tag="ACHK01_002073"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31954..31956,aa:Sec,seq:tca)
     gene            32093..32254
                     /gene="rhuM"
                     /locus_tag="ACHK01_002074"
                     /pseudo
     CDS             32093..32254
                     /gene="rhuM"
                     /locus_tag="ACHK01_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            32652..33836
                     /gene="setC"
                     /locus_tag="ACHK01_002075"
     CDS             32652..33836
                     /gene="setC"
                     /locus_tag="ACHK01_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO8097027.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANSQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            33947..34870
                     /gene="yicL"
                     /locus_tag="ACHK01_002076"
     CDS             33947..34870
                     /gene="yicL"
                     /locus_tag="ACHK01_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO8097028.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(34874..35692)
                     /gene="nlpA"
                     /locus_tag="ACHK01_002077"
     CDS             complement(34874..35692)
                     /gene="nlpA"
                     /locus_tag="ACHK01_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO8097029.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            35914..36207
                     /gene="yicS"
                     /locus_tag="ACHK01_002078"
     CDS             35914..36207
                     /gene="yicS"
                     /locus_tag="ACHK01_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO8097030.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(36248..37438)
                     /gene="nepI"
                     /locus_tag="ACHK01_002079"
     CDS             complement(36248..37438)
                     /gene="nepI"
                     /locus_tag="ACHK01_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO8097031.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(37649..38101)
                     /gene="yicN"
                     /locus_tag="ACHK01_002080"
     CDS             complement(37649..38101)
                     /gene="yicN"
                     /locus_tag="ACHK01_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO8097032.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(38154..39488)
                     /gene="adeQ"
                     /locus_tag="ACHK01_002081"
                     /gene_synonym="yicO"
     CDS             complement(38154..39488)
                     /gene="adeQ"
                     /locus_tag="ACHK01_002081"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO8097033.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         9988 a        10485 c         9627 g         9503 t
ORIGIN      
        1 ccacatccaa aaagaaaatt tctgtcaata ccaaatgcta tatcaagagt atttttagat
       61 cttatatttt gataatgaaa attatactca aaaaggatga tgtcactatc attgagttga
      121 gacatattta ttctgtatcc aaaattttgg caaaaataag ttacgggtta caacgatttt
      181 cgaatcgttc tttccctgat ttttgtggct tttgcttctg cttgcgacag cagtccttgt
      241 tcctgcttga gaatgtcttt aagcggggcg gcaaaataca ctttcataaa ccgccagata
      301 tcccgctgag tcaggccgat attcatcgaa gaaaaataaa gcccaataag atctttatcc
      361 cgccaacgac gtggaacgcg cgtgcgaagc tgcgcccggt gcaggtcaat taccgaaatt
      421 tttaactcct cttccttacc ggaaaaaggc aagtgcagca ggaaatgaca gatataacag
      481 tcacggtggt taatgcccgc agcatgcata tcgcgcacca tcgtcgcgac acgcttaata
      541 agcatacgct ttacgcgaac atctggaggg ttagtcgccc agtcagcact gtaatcttca
      601 agacttatgg ttggtgtcag atcttcggta ataataaatg aagttctggt cagcggattc
      661 atgccttttt cgccaaatgc caccccatac atagtatcaa cgccgacatc ccgcagtcga
      721 tgaatcgcat tccattcgcg gtcagcgcct aatactggca tccgcaatga gagtaaattt
      781 ttgattatct ctttcagggt cgtgccgcga tgccatttga gaaaatagct tttgcccgcc
      841 atttcaaagc gcagagtacg gcgagtttcc agttcacgaa atacctcacc ctgcaaggtt
      901 ttaacttcct caaaaggatc tttgccgcgc cataacgtgg caaacggctc tttaagttca
      961 accatccaga ccacccgtta tgatatccgc cgctttctct ggcagactgt ataaatcttg
     1021 tgtatcagca taatgtcgcg cattttccgc ccaggcctgg cgcaatgaag attgcgttaa
     1081 cgctttgcgt aaaatctcat tcaatgtttc ctggcggaat ggctcagcaa tagcctcgcc
     1141 gcaattagcg tcgacaatat aatgcgcata gccacaaacg gctgttgtta gtaccggtaa
     1201 tcctgcagta atcgcttcca gcagcacaat tcccgccgct tcctggtagg caggatgcag
     1261 taataaatcc gccgccgcca ttaattccga gacatcattg cgccccgaga aaaagtgaac
     1321 attactgcgc acgccgcgtt tttctgccag cgcctcaaat tttcgcggtt tatcctgccc
     1381 aacaacatat agcaatgtgt tgtggcgcag cgaatccggt aacgaagcaa gtgcttcaat
     1441 ggaacgatcg acacctttac gcgtgaagtc tgaaccgacc tgcaacaata aatattgttg
     1501 ttcggttatt ccattcttct tacggaagat ttcacggcta ttggctggct gctggctata
     1561 tttacgatca ggataaatcc ccggtggcag aatatgaaaa cgctccgctt cagtctgata
     1621 atgtttctgg aaatcggcga tttgcttatc tgtcagcatc agcaactgtg tcggcttacc
     1681 ctgttcgaag gttgcccgct caaaggcggc ataatggcga taacgtgacg tcaggcgata
     1741 gaaaaagcct ttttcctgcg ctactttctc ggcataacaa acatcagcgg cataataaac
     1801 gtccagcccc ggcattttgt tgaatccaac gactttatcg acgggatgtt cgcgtaaatg
     1861 tttttgcacc caggcaaaat actccgcatt gcgcccgtga ttggtatgcg atttaaccgg
     1921 cactttgatc agttcaaata catcagggca ttcgccttcc cacgactggg tataaacccg
     1981 aacatgatga cctcgggcgg cgactgtctg agcaatacgc ataaaatcac gctgcaaacc
     2041 gccaaaggga aaatatttat ataaacaaaa agcaacgatc ataacgacgt acctgtcgtg
     2101 gaggagggca gtaatttatc gacagcagca atgacatctg ccgccggaat aaccgaaaga
     2161 tacatctcat ttcggtcacg ctgatcgcgc gttggcattt cccggtaatc tcccgcccag
     2221 aattgaatca tgttatttga ccagggacgc cagaaaatat gatctgtcgc accaaacagc
     2281 gatatcagcg gcgtattaac tgcagcggca atatgcgccg gtgcggaatc aacgccaata
     2341 aacagctgcg catgatcgat taacgcacca agttccggga aggtcacctt tccagccagc
     2401 gccgttactg gtggcgtctg gcatccctgc gcaatttcat tgacgcaggc cagatcgtct
     2461 ttatctgggc cggacgtcag aacgacttca taaccacgag catgtaaggc atcaatcaca
     2521 gcggaaaact tggcgttgtc ccagcatttg aagatttgcc gcgccgtagg ttggataacc
     2581 acataatttt gtccaacacc agcgtgatca agttcgcgac gcatacgttt ccagcttgca
     2641 ggcgggtaac tcatggttgt ctgcttcacc aacgaatcaa gtcccaatgg tgtcagcacg
     2701 gataagttac tttccaccac atttccaccc tgcaacggca ccaaatgggt gaaactttta
     2761 cgccaaaaag cagactgccg atgatgataa tcctgggaaa ttttcacacg agcatttaat
     2821 aagcgaacca gtatagcaac catccattga tcggtaagat tgacgataag gtcatactta
     2881 ttggcacgta ataccttgat gagatgaaaa aagttggcaa ttttttctga ggcttttgct
     2941 tttttatttt ttatgccgta gagcgcgtta atctctggat tttcagacag gatcgggatg
     3001 gtgtcctgat aaagcagcac atcgattttt gcgtcagggt aatttttttt cagcgaacta
     3061 atgacgggag tagttaataa catatcccca tgaaaacgca tcttaatgag caaaattctt
     3121 cgaaatggct tatccacaag taactctttt tgttgagatt atccgattaa ggtgaagcag
     3181 aaaaagcacg ctaccgcccc aggctcaaca gctacctgaa tactgatacc gcactgatat
     3241 tgacgcgcta gtgtatcact tctttcgcgg caatccgacc cgaatgattt ttaagcacat
     3301 tgttgatttt caataaagaa atcaatcaaa taaaatcatt agcaaccata ttctttcact
     3361 gttgctctgc gattccttcc tgcacgtaca gcagaaatca atgaatcgca taaaaataca
     3421 aaaaaagtat agatgttcag caagaattgg cgggtaaatt gctatttaag tcaaaaacgg
     3481 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
     3541 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
     3601 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
     3661 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
     3721 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
     3781 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
     3841 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcact caaccgtttc
     3901 ctgaataaag tcgaccctaa actggtgttg attatggaaa ccgaactatg gcctaacctg
     3961 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
     4021 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
     4081 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
     4141 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac gccgcagttg
     4201 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
     4261 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
     4321 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
     4381 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
     4441 tccaccagca cgcaggttgt agttggcgat acgatgggcg agttgatgtt actgtacggc
     4501 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     4561 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     4621 atttgcgcgc ggctggagca ggcaagcggg ctgattaccg ttaccgatgc cactacgctt
     4681 gcaaaagagg tgtcctcttt actcaccgac gccgattacc gtagtttcta tggccgtcat
     4741 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     4801 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     4861 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcaaatgtt
     4921 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     4981 agagcgtgtg gcgctggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     5041 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     5101 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     5161 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     5221 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     5281 tgtccatcag gcgctgatgg cgaagttagc gtagcgttta tgccggatgg tatgccatcc
     5341 ggcgcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     5401 agtcgccaca atcggcgtac cgcacacccg acacggctcg ccttttcgcc cgtaaacctg
     5461 caattcctga gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     5521 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     5581 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     5641 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     5701 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     5761 aagcggctcc ggtccaagat gggtcagcac attatgccct tccagctctt tggtccacag
     5821 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttgccgttgc tcatcaccaa
     5881 ttccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     5941 catccctaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     6001 ccgctgcacg ctaagcactg gttggtcgct taaacggtag atctcttctg aaaccggcca
     6061 gcgcaagcgt ccgttgcgca ccactgcatg aagaatggtt gcaccaacga gatgcggttc
     6121 tatgccgcgg cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     6181 cagatgcagt caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     6241 tgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctggcgaa
     6301 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     6361 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     6421 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     6481 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     6541 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     6601 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     6661 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     6721 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     6781 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     6841 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     6901 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     6961 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     7021 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     7081 gtttattttt atcgcttcgc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     7141 gccggaaaaa agacggctat gcgttataac ccggtgttgg gagtcgagaa agatcaccat
     7201 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattccctcg tcatctccgg
     7261 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgccgcg ccagttcagc
     7321 aatccccttt aactgggcaa atttcgccac gccaattcca tgaacgccac taaattgttc
     7381 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     7441 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     7501 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgaatt
     7561 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt tcgttaaatc
     7621 gacggccagt tttcaatcct ggaaagcgcc tcgcaaagtg aatctcaggt gaaggcacga
     7681 caacaaaagt attgtgataa aatcgccaac ttctggtgtc accctacagg aaaaatcatc
     7741 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     7801 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     7861 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     7921 tccgacagtc tgctggaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     7981 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tattgctgcc
     8041 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     8101 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     8161 cttgcttccc gtggtatgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     8221 atcggtcctg ggcgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     8281 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggccc gacgcgtgaa
     8341 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     8401 gccgccgccg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc ggtttcacta
     8461 ccgacgccac cgtttgttaa acgtgttgat gtgatgactg cgctggaaat ggaagccgcc
     8521 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     8581 cgcgcagcta ccgtggcccc agagaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     8641 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     8701 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     8761 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     8821 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     8881 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     8941 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     9001 gacttatgcc acctctggct ctgccggact tgacctgcgt gtctgtctcg acgacgccgt
     9061 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     9121 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     9181 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     9241 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     9301 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     9361 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     9421 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     9481 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     9541 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     9601 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     9661 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     9721 gcatcaatct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     9781 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     9841 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     9901 aagcgcagct gcgtcaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     9961 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
    10021 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
    10081 ttgcggcaca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
    10141 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
    10201 tcttccaggt aagcaatcag gtctttcagg gtgatgatag agatcacttt gcagttgtaa
    10261 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cccgttcctg acgatcaagc
    10321 gaaatcaaca cgccagcaag cgtcgcgcca ttagcctgaa taatctccat cgattcgcga
    10381 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
    10441 ccaaccagat tgccgccttc accgtggtct tttgcctctt tgcggttaaa gcagtacggc
    10501 aggtcaaggt catgatgctc cgccagcgcc acggcggtag tggtggcaat cgggatccct
    10561 ttgtaagcag ggccaaacag cagatcgaac tcaaggccgg aatccaccaa cgcttcagcg
    10621 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
    10681 taggggcttt tgcgcccgga cttcaacgta aactcgccaa actttaacac ctgcttgcca
    10741 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
    10801 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
    10861 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagagccaac aaggtgagta
    10921 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
    10981 cttcggtcat cactacgttc atgtcggtct ctgcggcaga gtcttcaacg tattccagat
    11041 cgcaaaccgc ttcgccgttc acaattccga cagaaactgc ggctaccatc cctttcatcg
    11101 gattggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
    11161 aggcacccgt aatcgacgcg gtacgcgtgc caccatcagc ctgaagcacg tcgcagtcca
    11221 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
    11281 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
    11341 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
    11401 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaac actttggtat
    11461 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
    11521 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
    11581 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
    11641 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
    11701 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
    11761 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
    11821 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
    11881 cctgcgctat gagccagatg ttagcgcgca aggtgagctg atcctcaacg aaaaactggc
    11941 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
    12001 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atcttgacgc
    12061 cattgccgct gaaattctcg cggcgctgga tggtacgctg gacgacttta ttgtcgcgcg
    12121 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
    12181 cgaagtggtc aaagtccgcg cccatatgcc ggaaatcctg caatggcagc gtgagcgtct
    12241 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagct
    12301 ggttctgctg gcacaacgaa ttgacgttgc cgaagaactg gaccgcctgg aagcgcatgt
    12361 caaagagacc tacaacattc tgaagaaaaa agaagccgtt ggtcgtcgtc tggactttat
    12421 gatgcaggaa ttcaaccgcg agtcgaacac tcttgcgtcg aagtctatca atgccgaagt
    12481 gacaaactcc gcgattgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
    12541 cattgaataa ctcgtaacgc caattcttac ttttcagcct tcacaaatgc cgcccactca
    12601 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
    12661 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
    12721 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
    12781 atcatcaacc ttttgaagaa atacggcatt atggcactga agggcaagaa ttttggtcag
    12841 ctagagaatt ggcaccgtta ctggattatc gggactggcg caattttcaa aaggtactgg
    12901 cccgcgcgac tcaagcgtgc gaagccagca atcaagctgc atctgaccat ttcgttgaaa
    12961 ccaccaaaat ggttgtatta ggttcagggg ctcaacgaga acttgaagat gttcatctct
    13021 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc tgcgaaaccg gtcattgcgg
    13081 cagggcagac ttattttgct atccagaccc gacggcagga gcttgctgac gatgaagcat
    13141 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
    13201 acaaacaatt ggttgaggca gcacagtaag ctggggtagc gacagctact gatttcgcca
    13261 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
    13321 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
    13381 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat ggcgtgaatt
    13441 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    13501 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    13561 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa actaaaaact ctttttgttg
    13621 attgagatac ccgatgcgta agccaaagtc caggtgcggt gaacacaatg gctaaacatt
    13681 gcacctttct tttccacccc tcaattttaa cctatttttt cttatgcatt ttctcagaca
    13741 agaagtcaga agaatgcatc tacgctacag aaaatagcga tttcacataa ctacaagtta
    13801 tcaatttcct ctcaccttaa aaaatctcaa tcgtgacaat acgcacaaat cgctaccctg
    13861 ccagacagat ttttagggag agaaccatgc tgttacacat tttgtatttg gttggcatta
    13921 ctgccgaagc catgacgggg gctctggcgg ccgggcgacg ccgcatggat acatttggcg
    13981 taattattat tgcgaccgcc accgcaattg gcggagggtc agtgcgcgat attctgctgg
    14041 gccactatcc gctcggctgg gtcaaacacc cggaatatgt gattatcgtc gctaccgccg
    14101 cagtactcac cactatcgtt gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc
    14161 tcgatgcgct tggactggtg gtcttttcta tcatcggcgc acaggttgca ctggatatgg
    14221 ggcacggccc aattattgcc gttgtcgcgg cggtaaccac cggcgtgttt ggcggcgttt
    14281 tgcgcgatat gttctgtaaa cgtatcccac tggtattcca gaaagagttg tatgccggtg
    14341 tctcatttgc ctccgccgtg ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg
    14401 tggtaattat ctccaccctg gtattcggct tttttgcccg cttactggcg ctacgcctta
    14461 agctgggatt accggtcttt tactacagcc acgaaggaca ctaaggttca aaacctgtga
    14521 tctgctgggc agccagccaa ctgcccagct tcttgatttg cgcattttcc ttccattcag
    14581 taacctgtct ggcgcgcccc gatccagtcc ccggcagctg ctgccagaac tgctccgtgc
    14641 taaataaaag ttgcgaccag gaccgttcat cactggcatt aagcgccgcc cgggttagcg
    14701 gtattcccat tgccatcacc cagcgagtaa aaggctgctt acgagccaga ttaaaccgat
    14761 gccatagctg cgcactttta cttttcgcga tccccggcgt gttctgtaat tgctctggcg
    14821 ttaataaaag ccaggaaaag atatgctcaa agcgatgagt ctgatgcagc gcgcgccagc
    14881 cggcctcacc aataccatcc agccccagaa cctgttttga ccccagccag actaagcgtg
    14941 aaatgaactg ttcctgacaa acatcagaag caaagtagca ggtcaacgag ttaaagcggt
    15001 tttctggtgg tgtcggtttt gtacgttctg caccgcgcca gaccacatca tcaatgcgag
    15061 gaataccctg accggcaagg ctgacgagga tctgatcacc aggcgcaata tcccactcct
    15121 gccagcgcct gacggaacca atattcaccc gctggacttt tttatcatcc agcatgacag
    15181 gtgcgagtga cgcaaccacc gatattttac cgctcttacc caccgcaaac tgaatcgcct
    15241 tcacttcggc aacctgagct acaggttgat atttccaggc caccagccat tctgcctggc
    15301 ctggtagcca atggcgggat tcgggttctt tcgcccctcg cacaaccacg ccatcggtga
    15361 cgaagggtaa tttcgctttc caccactcat tgcgtacgcg cgcaacctca tcagcatttt
    15421 taaccgcacg ggtatacctc tgcgttagag taaaacctgc ggtagccagc tcctttaaac
    15481 gatcggtcat taactgcggt ccatccggcc atgcccagac aaaaacgccc agagaattca
    15541 gcgtgtcgct atcgtcctgg cgcatcatca ggccagcaac ttttgcgcgg gcatttattc
    15601 cccccatttg ttgttggata tgcccctcgc gctggagaaa tatttccccc tgaagcgtac
    15661 tgttggctaa aggcccgcta acggtttgcg gcacagcgga aattaagcta actttctgcg
    15721 tccagtcctc gcctttcagg ccattaccgc gactgattgc tttgttcagt ttcccgtccc
    15781 gataaaccag ggttaccgca acgccatcaa cttttggctg cacccaaaga tcgctacgtt
    15841 ctcgcatcca cagacttaat gcgtttttat ccgccatttt gcgtacaccc gtatgggcaa
    15901 ccggatgcat aaccgcacca tttaaaggcg gcatcatcac gtcgcggggc tcgctcacaa
    15961 agcagcgttg ccactgcgta agacgggcgc ttaactgatc gtaaacaccg tcttccacct
    16021 cactttttcc ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcgtg
    16081 aaatttcttc ctgtgctctg gctggcgacc aggccggaca gaccgcccac acagatgatt
    16141 gccagcacaa gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag
    16201 tcaggctggc agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc
    16261 gaggacgctt cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc
    16321 tacgcaaaac gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc
    16381 gccgtgtata ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc
    16441 atggctcaag gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg
    16501 attcaggctt tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc
    16561 acacgccaac cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat
    16621 gaatttaaag aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat
    16681 tactatggca cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt
    16741 ctcgatatcg actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt
    16801 atctttattt taccgccgtc caaaattgaa ctggaccgcc gtctacgcgg tcgcggtcag
    16861 gacagcgaag aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac
    16921 gccgaatatg attatctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag
    16981 accattattc gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta
    17041 atcagcaaat tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac
    17101 ctgtggagct ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt
    17161 aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga
    17221 aaggatccgc tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc
    17281 gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag
    17341 caggaagccg ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa
    17401 agcgggtcgc ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg
    17461 gaagaccaaa tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg
    17521 caaacacgtt caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg
    17581 gccgagatga aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa
    17641 gatactcccg ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg
    17701 gtagaggggg tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc
    17761 gaaaactttc gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa
    17821 cttgccgacc gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc
    17881 cgcatcgccc gtgaaactct cgaaatttat agcccgctgg cgcaccgttt aggtatccac
    17941 cacattaaaa ccgaactcga agagctgggt tttgaggcgc tgtatcccaa tcgttaccgc
    18001 gtaattaaag aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccaaaaaatc
    18061 ctctctgaaa tcgaagggcg tttgcaggaa gcgggaatac cgtgccgcgt cagtggtcgc
    18121 gaaaagcatc tttattcgat ttactgcaaa atggtgctca aagagcagcg ttttcactca
    18181 atcatggaca tctacgcttt ccgcgtgatc gtcaatgatt ctgacacctg ttatcgcgtg
    18241 ctgggccaga tgcacagcct gtacaagccg cgtccgggtc gcgtgaaaga ctatatcgcc
    18301 attcctaaag cgaacggcta tcagtcgttg cacacctcga tgattggccc gcacggcgtg
    18361 ccggttgagg tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc
    18421 gcgcactggg cttataaaga gcacggcgaa accagtacta ccgcacaaat ccgcgcccag
    18481 cgctggatgc aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc
    18541 gagagcgtta aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcgc
    18601 attgtcgagc tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat
    18661 atcggccatg cctgcgtggg cgcacgcgtc gaccgccagc cttacccgct gtcgcagccg
    18721 cttactagcg gtcagaccgt tgaaatcatt accgctccgg gcgctcgccc gaatgccgct
    18781 tggctgaact ttgtcgttag ctcgaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac
    18841 ctcaagcgtg atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt
    18901 agccgtaagc tcaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag
    18961 ctggcaacgc ttgacgatct gctggcagaa atcggacttg gtaacgcaat gagcgtggtg
    19021 gtcgcgaaaa atctgcaaca tggggacgcc tccattccac cggcaaccca aagccacgga
    19081 catctgccca ttaaaggtgc cgatggcgtg ctgatcacct ttgcgaaatg ctgccgccct
    19141 attcctggcg acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat
    19201 gaatcctgcc gtaatatccg tggctaccag aaagagccag agaagtttat ggctgtggaa
    19261 tgggataaag agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat
    19321 cagggcgcgc tggcaaacct gacggcggca attaacacca cgacttcgaa tattcaaagt
    19381 ttgaatacgg aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgcccgt
    19441 gaccgtgtgc atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa
    19501 gtcacccgaa accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa
    19561 atgctcgcca ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat
    19621 aacgtttctg cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc
    19681 tggcctggta gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta
    19741 caggtgaaaa cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg
    19801 cagattctgg caacccatct ttctgataac gctgtcgatt tccgcgaaat tgattacact
    19861 cgcccgacct gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc
    19921 ctggcggatc aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc
    19981 gttgcctcag ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac
    20041 ctgcgtgaaa acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc
    20101 tatccggtgc tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag
    20161 caaggcgaga tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg
    20221 ccatgaaagg tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag
    20281 cacttagtaa caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc
    20341 ttcccctgcg ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg
    20401 tttatgccac ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga
    20461 tgatgacctg ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca
    20521 gcgcggcaat gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa
    20581 agcgcggtaa atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca
    20641 gcacgccaga attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc
    20701 aggccacgct gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg
    20761 aagaactcct gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca
    20821 ctttgcaccg cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccgg
    20881 cgcaacgtcg tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac
    20941 gtgccggagc acagcgtttt catgcccagc cgctgagcgc caatgacacg ctgaaaaata
    21001 aactcctcgc cgccttaccg ttcaagcaaa cgggcgcaca ggcacgcgta gtggcggaga
    21061 tcgagcgcga tatggcgctg gatgtgccga tgatgcgtct ggtgcagggc gatgtaggtt
    21121 ccggtaaaac gctggtcgcc gccctcgccg cgttgcgtgc gattgcccac ggcaaacagg
    21181 tagcattgat ggcaccaacc gaattactcg ccgagcagca cgccaataac ttccgcaact
    21241 ggtttgcacc gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggtaaagcac
    21301 ggctggcaca gcaggaagcc atcgccagcg gtcaggtgca gatgattgtc ggtacacacg
    21361 ccatcttcca ggaacaggtg cagtttaacg gcctggcgct ggtgattatc gacgaacagc
    21421 atcgttttgg cgtacatcag cgtctggcat tgtgggagaa aggccagcag cagggcttcc
    21481 atccgcatca gttgatcatg accgccacgc cgatcccccg cacgctggca atgactgcgt
    21541 atgccgatct cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta
    21601 cggtcgctat tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca
    21661 taactgaagg tcgtcaggca tactgggttt gtacgttgat tgaagagtcg gaattgctgg
    21721 aagcgcaggc ggcggaagct acctgggaag agttgaaact ggcgctacca gagttgaacg
    21781 ttggcctggt acacgggcgg atgaaacctg ccgagaaaca ggcggtgatg gcgtcgttta
    21841 aacaaggtga gctacacctg ctggttgcca caaccgttat tgaagtcggc gttgatgtgc
    21901 ctaacgccag tctgatgatt atcgaaaacc cggagcgtct gggtctggcg cagttacacc
    21961 agcttcgcgg gcgtgtaggt cgtggtgcgg tggcttctca ctgcgtgctg ctctacaaaa
    22021 cgccgctctc caaaaccgcg caaattcggc tgcaagtgct gcgcgacagc aacgacggtt
    22081 ttgtgattgc acagaaagac ttagagattc gcggaccggg tgaactgtta ggtacgcgtc
    22141 agacgggtaa tgctgagttt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg
    22201 aagttcagcg cctggcgcga catattcacg aacgttaccc acaacaagca aaagccctga
    22261 tagaacgctg gatgccggag acggaacgtt actccaacgc atgaccagac tcatcacaag
    22321 acaagtcacg cagtaaatct tcaatctgga tagtgaggat atccattgcc tcatacgcat
    22381 cctgtgcaat tcgcgccgcc attgcgcctt ccggttcacc acgcagaatt tcagccgctg
    22441 cctgtacgcc agccacaatc atttcaggtg ttaacatttg gcgaagaaaa gcgcgattat
    22501 cagaggacca gccatcttta tggcctaatg gatgccccca gcctatccat tcttgcgacc
    22561 ataacggatt ggcttctatg ggataaatta gatgcgcggc atactgaata gcacacaccg
    22621 cttttcctgg cgtatccagc gcccaccagc gcgaccagag agaacgaatt aacggcgcgg
    22681 ccccacccag agtattgaac accgccagcc agcaaagaac gttattgaaa ccacgctcgt
    22741 tatccatacg ctgcaacgcg gtgtccagta aaaattgccg tacctgctgg cgttgggaag
    22801 cattcatcat ctcctgccag agatagggcc gacacaatgc cttcattaag gattcttcga
    22861 agtgatcacc ataaccgcca gctaaaacgg tttcatgcca ttgcgccagg cagagcggaa
    22921 acagatagcg aaacaactct atttgcatcg gttcgttata aacgagattg agtacatagt
    22981 aagcactcaa atcccattcg ctgatctgct gccactcttt agccgccagc gccttgagtt
    23041 cggcatctgc gtaatcaaat tccgtttccc atacacagag cactggcggc tcatcaccaa
    23101 aaatatggcg acagtgttca cgtagcgttt ccggttcctt ccgacgcttt ttcattttta
    23161 accggcaaaa atcggcaaca ttaaatacag cttaatcacc agcgcattga cgatatcgat
    23221 aaagaacgca ccgaccatcg gcaccaccaa aaacgccatg tgcgacgggc caaagcgttc
    23281 agtgatcgcc tgcatgttgg cgattgccgt tggcgttgca ccgaggccaa aaccacagtg
    23341 acccgcagcc agcactgccg catcgtagtt tttgcccatc atgcgccagg taacgaagat
    23401 ggcatacaac gccatgaaga tggtctgtac caccagaatc gccagcatcg gcagcgccag
    23461 cgaagccagc tcccacagtt tcagccccat cagcgccatc gccaggaaca acgacaggct
    23521 tacgttaccc agcacggata ccgcacgctc aaagacgcgg taaaagccca tcattgacag
    23581 accgttgctc agaatcacgc caacaaacag tacacagacg aaggtcggca gttcaaaagc
    23641 agtgccagcc aaaagttgcg caacaatttt ccccaccgtc aggcagatag caatcagcgc
    23701 gatagtttca atcagcacca gcgaggtgat catgcgtccc acatccggct tttcaaacgc
    23761 cgtcgggact tcctggtcat ccggaatacc gttcggcgtg gtggagtgtt tcaccagata
    23821 gcgcgccacc ggaccgccaa tcaagccgcc cagcaccaga ccgaacgttg cacaggccat
    23881 cgccacttcc gtcgcattgg tgaagccata acgttcaatg aacaatttac tccacgcagc
    23941 gcccgtaccg tgaccgccgg aaagcgtaat agaaccggcc aacagcccca tcagcggatc
    24001 aagccctaac aggctagcca taccaatgcc aatggcattt tgcatcacca acagaccaac
    24061 aaccacaatc aagaagatgc caaccacacg cccaccggca cgcaaactgg caatgttggc
    24121 gttcaggcca atggtggcga agaaagccag cattaacgga tcgcgcaggg acatatcaaa
    24181 gttgacttcc cagcccatgc tttttttcag tactagtagc gccagcgcca ccaacaaacc
    24241 acccgcaaca ggttccggta tggtgtattt cttcaaaaag gagacggaat ggaccaactt
    24301 acgcccgagc agcaacgtca gcgttgcggc aacaagcgtt gctaaagtat cgagatgaaa
    24361 catagttact cctttgtatc cgcatcactc tttcatactc gtcatacttc atcgcgcagt
    24421 ggcaacttca tgaactatga cgggtatcga gttatgcttt cctgggtcaa attctcagct
    24481 gttaatgaac aaatggcatg gattttaagc agaaaaatac caaaagttat ataaaaaagt
    24541 gcatttatct catttaaata tttagatgag gatgtggcaa acgtttgctt ttctctttcg
    24601 gtcagctaaa atgcccgctt tgctaccacg ggattgtttt cgatgtctgt ttccaccctc
    24661 gagtcagaaa atgcgcaacc ggttgcgcag actcaaaaca gcgaactgat ttaccgtctt
    24721 gaagatcgtc cgccgcttcc tcaaaccctg tttgccgcct gtcagcatct gctggcgatg
    24781 ttcgttgcgg tgatcacgcc agcgctatta atctgccagg cgctgggttt accggcacaa
    24841 gacacgcaac acattattag tatgtcgctg tttgcctccg gtgtggcatc gattattcaa
    24901 attaaggcct ggggtccggt tggctccggg ctgttgtcta ttcagggcac cagcttcaac
    24961 tttgttgccc cgctgattat gggcggtacc gcgctgaaaa ccggtggtgc tgatgttcct
    25021 accatgatgg cggctttgtt cggcacgttg atgctggcaa gttgcaccga gatggtgatc
    25081 tcccgcgttc tgcatctggc gcgccgcatt attacgccgc tggtttctgg cgttgtggtg
    25141 atgattatcg gcctgtcgct aattcaggtt gggttaacgt ccattggcgg cggttacgca
    25201 gccatgagcg ataacacctt cggcgcaccg aaaaatctgc tgctggcagg cgtggtctta
    25261 gccttaatta tcctgcttaa ccgtcaacgt aacccttact tacgcgtggc ctcactggtg
    25321 attgcgatgg cggccggata tgcgctggcg tggtttatgg gcatgttgcc agaaagcaac
    25381 gaaccgatga cgcaagaact gattatggtg ccaacgccgc tctattacgg tcttggcatt
    25441 gaatggagtc tgctgctgcc gctgatgctg gtctttatga tcacttcgct ggaaaccatt
    25501 ggcgatatca cggcgacctc tgacgtttcc gaacagccag tgtccggtcc gctgtacatg
    25561 aaacgcctga aaggcggcgt gctggcaaac ggcctgaact cgtttgtttc ggcggtgttt
    25621 aacaccttcc cgaactcctg cttcgggcaa aacaacggag tgatccagtt gactggtgtt
    25681 gccagccgct atgtcggttt tgtcgtcgcg ctgatgttga tcgtgctggg tctgttcccg
    25741 gcagtgagcg gttttgtaca acacattcca gaaccggttc tgggcggcgc aacgcttgta
    25801 atgtttggca ccatcgccgc ctccggtgtg cgtatcgttt ctcgtgagcc gctgaaccgt
    25861 cgggcgattc tgattatcgc gctgtcgctg gcggttggtc tgggcgtgtc tcagcagccg
    25921 ctgattttgc agtttgcccc tgaatggctg aaaaacctgc tctcctccgg gatcgccgcg
    25981 ggcggtatta ctgccatcgt gctgaatctg attttcccac cagaaaaaca gtaattcact
    26041 ggcggcggta agataattac cgccgcagtc tcacctcttt caccattccc gccttgagga
    26101 ttgcgcgtaa atcgtgcata actcccttat gtgcatttca cgggatggaa gaccatgaaa
    26161 tttattggga agctgcttct ctacattctc atcgctctgt tagtggtgat cgctggcctc
    26221 tattttcttc tgcaaacccg ctggggagca gaacatatca gcgcatgggt ttccgagaat
    26281 agcgactatc atctggcctt cggggcgatg gatcaccgtt tttccgcgcc atctcatatc
    26341 gtgctggaga acgtcacgtt tggtcgtgat ggtcagcccg cgaccctggt ggcaaaaagt
    26401 gtcgacattg cgctaagcag tcggcaactg accgaaccac gccatgtcga taccatcctg
    26461 ctggaaaacg ggacgctgaa tctcaccgac cagaccgcgc cgctaccgtt caaagccgat
    26521 cgtctgcaac tgcgtgatat ggcgtttaat agcccgaata gcgaatggaa actgagcgcg
    26581 cagcgggtaa atggcggcgt ggttccgtgg tcaccagaag ccggtaaagt gctgggtacg
    26641 aaggcgcaga ttcagtttag tgccggatcg ctttcgctca atgatgttcc tgccaccaat
    26701 gtactgattg aaggcagtat tgataacgat cgcgttacgc tgactaacct gggtgccgac
    26761 atcgcccgcg ggacattaac cggaaacgcg cagcgtaacg ccgacggcag ctggcaagtg
    26821 gaaaatctgc gcatggcgga tatccgtcta caaagcgaaa aatcgctaac cgacttcttt
    26881 gcgccattac gctctgtccc gtcgttgcag attggtcgcc tggaagtgat cgatgctcgt
    26941 ttgcaaggtc cggactgggc ggtgaccgac ctcgatctca gcttgcgcaa catgaccttc
    27001 agtaaagatg actggcagac acaagaaggc aaactgtcga tgaacgccag cgagttcatt
    27061 tatggttcgc tgcatttatt tgacccgatt ataaacgcgg aattttcccc gcagggcgta
    27121 gcgctgcgcc agttcaccag ccgctgggaa gggggtatgg tcagaacgtc agggaactgg
    27181 ctgcgtgacg ggaaaacgtt gatccttgat gatgcggcaa ttgccgggct ggaatatacc
    27241 ttgccgaaaa actggcaaca gttgtggatg gaaacgacac ccggttggtt aaacagcctg
    27301 caactgaaga gatttagcgc cagccgcaat ctgatcattg atatcgaccc tgacttcccg
    27361 tggcagctca ccgcgctcga tggttacggt gccaacctga cgctggttac cgatcataaa
    27421 tggggcgtct ggagtggctc ggcgaatctg aatgccgccg ccgcgacatt caatcgtgtt
    27481 gatgttcgtc gcccgtcgct ggcgctgacc gccaacagca gcacggtgaa tatcagcgaa
    27541 ctgagtgcat ttactgaaaa aggcattctg gaagccaccg ccagtgtttc acaaacgcca
    27601 caacgtcaga cacatatcag cctgaatgga cgcggtgtgc cggtgaatat tttgcaacag
    27661 tggggatggc ctaaattacc gttgactggc gacggcaata ttcagcttac cgccagtggc
    27721 gatattcagg ccaatgtccc gttgaaacct acggttagcg ggcaattgca tgccgtgaac
    27781 gccgcaaagc agcaagtgac tcaaaccatg aatgctggca tcgtttccag cggtgaagtt
    27841 acatcgacgg agccggtgcg gtaaacatca atctcgccgg gtacgactca gacgtacccg
    27901 gcattccatc aatagattac aacgtaattg tcagcgcatt cccttgaggc ttcaccacca
    27961 gcccctgctc actttcagcc tgcgaaccgt cttgcagacc atttactttc acaacattgc
    28021 gcaggcacag cgtccagttc ttcgcctcgc ccgcaccagt cacagtaatc gtgttgccag
    28081 tacgtgctgc ttttaaagta aagatcaccg atccgtcagt agcgggcact tcacagacgg
    28141 cttcatgccc gtcttgcaga ttaaagaggt ggaatgccgt gccttcgtgc cacacgtaat
    28201 cgggacgttg atcgttgttg cccagcgcca gtagagtgtt atcacgcaca taaacgggca
    28261 gactcaggaa gccgtgctgc tgtttatgcc agcgactacc gtcgagttca tcgttgtgcc
    28321 acaggtgtgt ccagcgacct tccggcaggt agaactgcac atcgcccgct tcagtgaaca
    28381 ccggcgcaac catcacgttg tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg
    28441 gatcgtccgg gaactccatc atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac
    28501 gcgcagcttc acgatacaga tacggcatca tgcggcattt cagttgcgtg aagaagcgca
    28561 ccacatcaca ggactcatca tcgtaggccc acggcacacg ataagatttg ctaccgtgta
    28621 aacggctatg gctggagagc aaaccaaacg cgcaccagcg tttgtaaacg tgcgccggag
    28681 cggtattttc aaagccgccg atatcgtggc tccagaagcc aaaacctgaa aggccaatag
    28741 acaaaccacc gcgcaggctt tccgccattg attcgtagtt agcgtaacaa tcgccacccc
    28801 agtgtaccgg gaatttctgc gcaccgacgg aggccgagcg ggcaaacaag acagcttctt
    28861 cctcaccaac ggtgtccttg agcacgttcc acaccagttc gttgtagatg tacgcataat
    28921 ggttatgcat tttctgcgga tcggaaccgt caaaccactg aacatcagtt gggatacgtt
    28981 cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac cagacctttc agtttgtcgg
    29041 cgtaccattt gcaggcatcc ggattggtaa agtcataaat cgccagacct ggctgccatt
    29101 tatcccactg ccatagcgaa ccgtcctggc gtttgagtaa atagcctttc tcttgtaact
    29161 ctttaaagac gggggatttt tgaccgatat aggggttaat ccagacgcag attttcagtc
    29221 ctttcgcttt caggcggcgg atcatccctt ccgggtcagg gaaagtcagc gggtcccact
    29281 caaaatcgca ccactggaag gctttcatcc agaaacagtc aaagtggaaa acatgcagcg
    29341 gcagattgcg ttccgccata ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg
    29401 tggtaaatga agtggttagc cacaggccga aggaccacgc gggcggcagc gccggacgac
    29461 cagtaaagcg ggtataacga tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt
    29521 attcgagata ttcactctca acgctgaact gcactttgga gactttctcc gatcccactt
    29581 caaaagagac acactgggga tgattgacca gtaccccata accacggtta gtcatgtaga
    29641 acgggatatt tttatacgcc tgttcagtac ttgtgccgcc gtcccggttc caggtctcta
    29701 ccgtctggcc attgcgcacc agggcagtaa agcgctctcc cagaccgtaa actgtttcgc
    29761 caacgccaag atcaagccgc tcaaacatat aattgcgttg attattcgtg tcctgcacgt
    29821 agccattatt tttcacctga ctaccggtaa tacgttcgcc gttgcgcaga aaatccagtg
    29881 accagaactc acctttgctg acacgcgcgc ttaagttgcc acttttaaac tcagcataac
    29941 gttctgtgtt ttcgattgtg accttcacgt cctgcaaaat attgagcgga taatgaggac
    30001 cgttattcag cgccccctga aaatgctcaa tccgcacacc gacaatacct tcctgtgggg
    30061 agaaaaagcg caacgtaaat aaaggcgtat caagctgcca ggtacgttca cgcacatcac
    30121 ggggggcagc atagaccacc atttcattat cctgctgttc aacctcgaac acctgaagcg
    30181 ggtgaatcaa attgaggcca ggttgaatca accagtttcc atcgctaatt ttcattgccg
    30241 ttccttagtt ctgcacttct tgagaggtga attgttgctg gcaacggcgt ttaccctgag
    30301 ccagctgttc cataacggtt ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga
    30361 taatcgcgct cagcaaataa cagatcgccg gaacaatcgt gaatagcgca ataatgatgc
    30421 taatcgtggc gctgttctgc gctttttctg ccgcatcata tccgccataa gccagcatcc
    30481 agccgataag agcgccgccg aaggccaacc ccagtttgag cacaaacagc gtgccagcaa
    30541 aactgatccc ggtcaggcgt ttaccattgc accactcgcc gtagtcgacg gtatcggaca
    30601 tcattaccca ctggataggt gtcaccagtt gatgcaacac accaatcacg aagatgaaga
    30661 caaacatagt gatgctggcc tgcatgggaa caaagaacat cgcgaggcta atcactgcca
    30721 gcagggcgtt cgtccaccag aagatagtga ctttacattt ccagtcggtc agaggttttg
    30781 ccagtgcgga accaatcagg ttacccacgc aataagtggt gagaaaagcg acaaacactt
    30841 ccggcgtgcc caaaatccat gtgacgtaat acatcatcgc cccaccgcgt acgcacaccg
    30901 ccaggatatt gaaaatggtt agtaaaccga caatccgcca ctggtcgttt tgccagatat
    30961 cacgtaaatc ttcccgcata gacgttgttg taggtggtgc ttcaacgcgt tctttagtgg
    31021 tgaagaaaca aaatgccagc atcatgaatg ccaccacgga aaggaccgcg ataccgccct
    31081 ggaaaccgag tggtttatta tcaccgccaa ttaaattaac cagtggcatc atcagaacag
    31141 tagaaagcat gcctcccgcg gtcgccagca caaaacgcca ggattgcagc gagatacgct
    31201 gagtcgggtc attggtgatt acaccaccca atgcgcagta agggatattg acgacggtat
    31261 aaagtaaggt aagtagggtg taagtaattg ctgcatagat cattttgccg ttcatactga
    31321 gatctggcgt gctataggcc agtacacaga cgatcccgaa tggcagtgcg ccaaacagta
    31381 cccacggacg aaatttaccc cagcgagagc gcgttcgatc ggccaacaac cccatgcaag
    31441 gatcggaaat cgcatccagt gcacgagcga ccaaaaacat ggttccgaca aaaccggcag
    31501 gaatgccaaa aatatcggta taaaagaaca tcatatataa cattacgtta tcgaaaataa
    31561 tgtggctggc ggcgtctccc atgccataac caattttctc tttaacggac aacacttcac
    31621 tcttcatctg cttttccttg atgctatagg ggtgctgaga ccgataacgc attttgtaaa
    31681 ccatccctgt gagagcgaat attccgatat ctggttatta atttatgatt cttgttttat
    31741 gtgatcgtgg tagcgttaat ttcgctcata tatcattgta aaatataggt tttatatgaa
    31801 ctataatgct ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg atctggggga
    31861 tgtagaaact caaggaagta gctataatgc gccccgcctc catgtagcaa tcgaggcgcg
    31921 gaagatcgtc gtctccggtg aggcggctgg acttcaaatc cagttggggc cgccagcggt
    31981 cccgggcagg ttcgactcct gtgatcttcc gccaaaatgc ctctcctgac gttctccaac
    32041 cccttgtaat actgtcaatc cagatatttt ctactgctca atattcaatc ggcttcagga
    32101 atacctggtc taaggttttg tgatggacga tgagcggctg aagaatccgc ctgtcggttc
    32161 ttctgtcctc gaacgcatcc gcgatattca cgccagtatg cgccgtgttt atttgcaggt
    32221 gatttgcccc cctgctgact ctcagccaac tgtatcgcca ttaaggtgaa gatgttgttt
    32281 tactcgcccc agaacaccct tttttatacg actattctat ttctttaacc gctggcgaaa
    32341 aaaaactgac tataaacttc accaataatc agactgccat caagattaat ttgttcattt
    32401 aatatatcaa atcaacttgt tttattttta ctatcaaaaa tagctcattg ccttactata
    32461 attaatatat aaattcggta attaattctt aacatgcttt tgctaataat ctcaattgct
    32521 ggccctataa tattgcgcta gcattgcttt ctggttgtat cagcgatact caaaaattct
    32581 taatacaata ctcattcgac tggtacttat ttgtaactca gttatatttt ttcgcccggt
    32641 gattcagaag aatgcaaaaa acggctacca ctccatcaaa aatacttgat ctcactgccg
    32701 cggcattttt acttgtcgcc tttctgacgg gtattgcggg cgctcttcag actcctaccc
    32761 taagtatatt cctcgcagat gaactgaaag cccgtcctat aatggtaggt tttttcttca
    32821 ccggtagcgc tattatggga attctggtca gtcaatttct ggcaaggcac tccgataaac
    32881 aaggcgaccg taaattactg attctgctat gttgcttatt tggagtgctg gcctgcacgc
    32941 tttttgcgtg gaatcgcaac tacttcattc tgctctcaac gggcgtactt ctgagtagtt
    33001 ttgcttctac cgcaaactcg caaatgttcg ccctcgcccg tgaacacgcc gacagaacag
    33061 gccgtgagac ggttatgttc agtacatttt tacgtgctca gatctcgctt gcctgggtta
    33121 tcgggccacc gcttgcttat gaactggcaa tgggatttag ttttaaagtg atgtatctca
    33181 ccgctgccat cgcatttgtt gtttgcggac tgatagtctg gttgtttttg ccatcaatac
    33241 aaagaaatat tcctgtcgtt acccaacccg tagaaatttt accctccacc cacaggaagc
    33301 gggatacgcg gctacttttt gtggtctgtt caatgatgtg ggcggcgaat aatctctaca
    33361 tgataaatat gccgctattt attattgatg aactgcatct aaccgataaa ctggctggag
    33421 aaatgattgg tatcgctgcc ggtctggaaa ttccgatgat gttaatcgca ggctattaca
    33481 tgaaacgtat tggcaagcgg ctattaatgc tcattgctat cgtgagtgga atgtgttttt
    33541 acgccagcgt actcatggcg acgactccgg cggttgagct ggaattgcaa attcttaatg
    33601 ccatcttcct tggtattctc tgtggtatcg gcatgcttta ttttcaggac ttgatgcctg
    33661 aaaaaatagg ctctgcgaca acgttatatg caaatacttc acgcgtcggc tggattatcg
    33721 ccggctctgt tgacggaatt atggttgaaa tctggagcta ccatgcgttg ttctggctgg
    33781 cgatagggat gttgggtatt gcgatgattt gcctgctgtt tattaaagat atttagctca
    33841 ccagtctggg ctaagaaaaa tcggcgtggc gcaaacagag ccatcctcgc catttttgct
    33901 atcatgcctg catacataaa cgacaaaaca gtatgcagag ggaaaaatgg gttccaccag
    33961 aaaggggatg ctgaacgttc tgattgccgc cgtgttgtgg ggaagttcag gggtctgcgc
    34021 gcaatacatc atggagcaaa gccagatgtc gtcgcagttt ttgactatga cgcgtttgat
    34081 attcgccggt ttgattctac tgacgctgtc atttgttcat ggcgataaaa tcttttctat
    34141 tattaacaat cataaagatg ccattagcct gctgattttt tccgtggttg gcgcgctaac
    34201 tgtacagctc acttttttgc taaccatcga aaaatcgaac gcagccacgg caacggtgct
    34261 gcaattcctc tcaccgacga ttatcgtcgc ctggttctca ctggtgcgta aatcgcgccc
    34321 gggcattctg gttttctgcg ctattttgac atcgctggtc gggacttttt tattggtgac
    34381 acacggtaat ccgacgtcat tatcgatctc tcctgccgcg ttgttctggg gcattgcctc
    34441 ggcatttgct gctgcattct ataccaccta tccctcaacg ctaattgccc gctatggcac
    34501 gttaccagtc gtcggctgga gtatgctgat tggcggtctg attctgttgc ctttttatgc
    34561 cagacaagga acaaactttg tcgttaacgg cagtttgatt ctggcgtttt tttatttggt
    34621 ggtcattggt acgtccctga catttagtct gtacctgaaa ggagcacaat taattggcgg
    34681 tccaaaagcc agcattttga gctgtgcaga accattaagt agcgcgctac tctctttgct
    34741 gttgctgggg atcacgttca cattaccgga ctggctggga acgctgctga ttctgtcatc
    34801 ggtgattttg atttcaatgg attcccgtcg ccgcgccaga aaaataaatc gtccggcgcg
    34861 gcatgagtga gaattaccag ccaggcaccg cgccaccgtt aaaaatggtt tctgccgctt
    34921 tagcgacttc gggtgattga taagattgca gaaattcctt cacgttttct gcattcttat
    34981 tatcttcccg tgccaccaaa atattcacat acggcgaatt cttatcttca ataaatacgc
    35041 tgtcgtgcac cggagaaagc cctgtctgct gaatgtaagt ggtactgata atcgctacat
    35101 caactttggg atcatccagt acgcgcggaa gctgcgcccc ctcgagttcc ataatctgca
    35161 aatgacgagg attatcggtt atatccagcg cggtaggtaa taaccctttt ccctctttca
    35221 gagtaattag cttctctttt tgcaacagta aaagtgcgcg accaaggttg gtcgggtcgt
    35281 tggggatcgc cactgtcgcg ccttctttta tttgcgccac ggttttgatt tttttggaat
    35341 aacccgccat agggaagaca aacgtatttc ccaccgctac cagtttataa ccatgcgcct
    35401 gattatcttg ttcaagaaag ggtcgatgct ggaagacatt cgcatcaagt tcgccatgat
    35461 ttgtcgcatc gttcggtagc aacgaaccgc taaatcctac cagttcaaca tcgaggccat
    35521 atttctcttt tgccaccttt ttagcgactt ccgcgacatc ttgttctgcg ccatttataa
    35581 cgccaacttt aatgtgtttt gcatcgctgc tactctggtc gcaacctgcc agcagaattc
    35641 cggccagcaa taatgcggcc cctgtccgta gatgatgtgt tgtcagtttc atttttttat
    35701 ccttttatta atgattacct gactatagcg gtaaggtcgc tgcggtttaa aaaacgaaac
    35761 gctatcgata agaataaaaa ggaataaaag tggaatataa agtctggcac ttcttactga
    35821 ccacgcaagc gcgtttcgta cagcatgatg agagcgatga aagcaaacta cacttatgct
    35881 tcattcgata cacatttgta aaaggttgaa tccatgaaac caacgacact actgcttatt
    35941 ttcacttttt tcgctatgcc aggcatcgta tacgccgagt cgccttttag ctcactgcag
    36001 tccgcaaaag agaaaaccac ggtattacaa gatttacgta aaatttgcac gccacaggcg
    36061 tcattatcag atgaagcgtg ggaaaagtta atgttgtcag atgaaaacaa taaacaacat
    36121 atacgcgagg ccatcgtggc gatggaacgt aataatcaga gcaattattg ggaagcgttg
    36181 ggtaaggtag aatgcccgga tatgtaagaa cagcaacaac tactgaccaa cgcttcttta
    36241 cctgatatca ggatttcttc attttcacct ttgcagtaac caacaatgca gtcagcaaca
    36301 tcaatgtgcc ggacaacatc aacggcgaag tcagaccaat attatccagc gcataaccgc
    36361 cgattgccgc gccacaggta ttagcaagct gaataaccgc cacctgaata gacccggctt
    36421 tttctgcctg atcggccagc gagcgggtga tccacgttga ccagccgacg ggaaccaatg
    36481 caaaagttag cccccagata atcgccacgc cggtagcaac gattttatcg cttccccaca
    36541 acgtcagtac caacgcactc acagccagta ttaacggcgc gcctgctaag gccagtttta
    36601 ccgaacgttt aagaatgaat gacgaaagcg acgtaccgat aaagctggca atgccaaaac
    36661 tcaacagcac cagcgttaag ccatccacgc tgaatcccgc caggttcata tacaccgggc
    36721 gaatatatgt gaagaaagca aactgcccgg cgaaagacat gaagatggcg atcatccctg
    36781 ccatcacacc cggacgttgt aataagcgga aagtattttg tttctgatgc gagggttcgc
    36841 ctggcagtga aggcaatgat ttgataatcc agaaaataca cagcacgccc atcaccgccg
    36901 ccgcattaaa gacattgcgc caaccgataa gctcgcctaa aaaactgccc aacggcgcgg
    36961 caatcaccag cgcaatagaa accgcgccga agatcaccga cagcgccttc ggcaccgtac
    37021 gcggcggcac cagacgcatg gtcagcgacg ccgacatcgc ccagaacccg cccagcgcca
    37081 gccccagaca ggcacgaccg attaaaagca aactgaatga gttagcaaag gaaaccagca
    37141 agcaggagag cgtcagcaaa acggcaaaca aaataacaac gtagcggcgg tcagtagcct
    37201 gaattgtctg ggtaataaac aaactggcaa acattgccac aaaggcggtc acggtcaccg
    37261 attgcccggc aaccccttcc gaaatgccta aatcctgggc cattggcgtc aacaaactga
    37321 cgggcaaaaa ctcaacgata atcagacagg cgacacaaaa cgccaccgag aaaacggctg
    37381 accagttcgg tcgggtgatg gcatccgcgc cgcggttttc ggcaataaat tcactcatgg
    37441 tgttacccgt ggcatgtttc aggggaaaag ccgaccactt taacattgaa atgtgtgacg
    37501 cacttaacgt ttttgcaact tttacggtgt tgcttgagct ggagttaaga tcgaaacgga
    37561 acagggcaaa gattaccgga tgcggcgtga acgtcttatc cacccgacag gtcaggcgcg
    37621 gtctgtaggc tgataagacg aagcggtttt aatgcagcca gtgaacgccc tcggcaggct
    37681 gaaagaacgc gttatagcgc atctgaaaag cattaatatc ctgcatttca ggttcggttt
    37741 cgcgcagaaa acccagcgcc agcctgacct gagcatcggt aatcggcgcg gcaagacggg
    37801 ctttttgtaa aatccgatgc cattgcagca ggttctgttc gctaccatcg acaatgacaa
    37861 tttgccagat gagcaacacc tgcgcggcgt tttgcaggtg cgactcatcc gggcgatgca
    37921 aataacgtaa aaattcgtca ccggctgact ttcccatttg atcgaccatc gactccacgg
    37981 gtacgacatc gatattcagc cgatcgctga gacggcgaat cgcttttctg gccccggatg
    38041 tcagcacccg aaaaccaacc acaaacacta ccgccagcgt ggcgagcatt atccagatca
    38101 tgagttctcc tgcgtaatca gaactcatga tatctggaaa ccctcgccag ttactaatcc
    38161 accagaataa tcttcagtgc aaacagagct gcgaccacca ccacacacag gttcagatcg
    38221 cgccagcgcc cggtgcacac tttcatgatg cagtacgaca taaagccgag tgcaatccct
    38281 tcggtgatcg aaaaagtaaa gggcatcatc accgtggtaa taaacgcagg caccgattcg
    38341 gtaaaatcat cccagttaac gcgcgccagg ctcgaagtca tcagcacgcc aacaaagatt
    38401 aacgctccgg cggttgcgta aggaggaacc atcgccacca gcggtgagaa gaacataact
    38461 aacaggaaca taacgccaac cacaaccgca gtcagccccg tgcgaccacc gactgccaca
    38521 ccagaagtac tttcaatata ggcagtaaca gacgaggtgc cgataaacgc ccccgccacc
    38581 gaactgacgc tatcaacata cagcgcctta ttcatattgg ggaatttacc gttactatct
    38641 attaagcccg ctttatcagt tacaccaatt aatgttcctg atgaatcaaa taggttgatc
    38701 agcataaagg agaaaatgat accggcgagt tcaagtgtta acgcgccgct caaatctact
    38761 tcaccaatga cgccgctaat atcaggcgga atggaataga cgccgctaaa atgaacatca
    38821 ccgaaaaata atccacagca agacgtcacc acaatagaaa ccagcaccgc ggcatgaaaa
    38881 tgacgtgatg acaacacggt tataataaaa aaccctaaaa tacctaacaa cacgccgtga
    38941 gaacttaaat cgccaatcat caccagcgtg tctttattgg cgacaataac gccagtattt
    39001 tttaatccca ttaaggcgat aaataatcca attccgctgg tgataccaat acgtaaactt
    39061 aagggaatgt tggagatcat ccagtaccgg atacgaaaca gcgtgagcaa aaatagtcca
    39121 actgcgcccc agaatattgc gcccatcccg gtctgccagg agatgcccat cgcccccacg
    39181 accacgaagg caaagaaggc gttcagcccc attgccggag ccagcgccac gggtaagtta
    39241 gcaaatatcc ccatcgcaat actgccgata ccggcaatca aacaggtggt aacaaacacc
    39301 actttcgggt ccatttgtgc cgcgccgagg atttgcgggt tcacaaaaac gatgtacacc
    39361 atggttaaaa aagtggtcat ccccgcaatc aattctgtgc ggacggtggt cccatgctga
    39421 ggtagtttaa ataatcgcga aagcgtccct gattcattac tcacgtaatc ggtattgtca
    39481 ttattcattt ttttgtccat tggaggagat ttaatccctt gcttttgtaa aacaaatgac
    39541 aacaacggtt cagtgataat tatcacattt caattgcaca ttaatggata ttctttaata
    39601 atc
//
LOCUS       DAZGCS010000039        39315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGCS010000039 DAZGCS010000000
VERSION     DAZGCS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.039"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            214..633
                     /gene="umuD"
                     /locus_tag="ACHK01_002082"
     CDS             214..633
                     /gene="umuD"
                     /locus_tag="ACHK01_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO8097034.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            633..1901
                     /gene="umuC"
                     /locus_tag="ACHK01_002083"
     CDS             633..1901
                     /gene="umuC"
                     /locus_tag="ACHK01_002083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO8097035.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="ACHK01_002084"
     CDS             complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="ACHK01_002084"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO8097036.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="ACHK01_002085"
     CDS             complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="ACHK01_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO8097037.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4386..5105
                     /gene="fadR"
                     /locus_tag="ACHK01_002086"
     CDS             4386..5105
                     /gene="fadR"
                     /locus_tag="ACHK01_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO8097038.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5157..6689)
                     /gene="ycgB"
                     /locus_tag="ACHK01_002087"
     CDS             complement(5157..6689)
                     /gene="ycgB"
                     /locus_tag="ACHK01_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO8097039.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7019..8317
                     /gene="dadA"
                     /locus_tag="ACHK01_002088"
     CDS             7019..8317
                     /gene="dadA"
                     /locus_tag="ACHK01_002088"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO8097040.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8327..9397
                     /gene="dadX"
                     /locus_tag="ACHK01_002089"
     CDS             8327..9397
                     /gene="dadX"
                     /locus_tag="ACHK01_002089"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO8097041.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9783..11519)
                     /gene="cvrA"
                     /locus_tag="ACHK01_002090"
     CDS             complement(9783..11519)
                     /gene="cvrA"
                     /locus_tag="ACHK01_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO8097042.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11614..12528)
                     /gene="ldcA"
                     /locus_tag="ACHK01_002091"
     CDS             complement(11614..12528)
                     /gene="ldcA"
                     /locus_tag="ACHK01_002091"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO8097043.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12628..13239
                     /gene="emtA"
                     /locus_tag="ACHK01_002092"
     CDS             12628..13239
                     /gene="emtA"
                     /locus_tag="ACHK01_002092"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO8097044.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13241..13975)
                     /gene="ycgR"
                     /locus_tag="ACHK01_002093"
     CDS             complement(13241..13975)
                     /gene="ycgR"
                     /locus_tag="ACHK01_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO8097045.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14176..14430
                     /gene="ymgE"
                     /locus_tag="ACHK01_002094"
     CDS             14176..14430
                     /gene="ymgE"
                     /locus_tag="ACHK01_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8097046.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14608..15048
                     /gene="ycgY"
                     /locus_tag="ACHK01_002095"
     CDS             14608..15048
                     /gene="ycgY"
                     /locus_tag="ACHK01_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO8097047.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15127..16824)
                     /gene="treA"
                     /locus_tag="ACHK01_002096"
     CDS             complement(15127..16824)
                     /gene="treA"
                     /locus_tag="ACHK01_002096"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8097048.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17144..18562)
                     /gene="dhaM"
                     /locus_tag="ACHK01_002097"
     CDS             complement(17144..18562)
                     /gene="dhaM"
                     /locus_tag="ACHK01_002097"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO8097049.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(18573..19205)
                     /gene="dhaL"
                     /locus_tag="ACHK01_002098"
     CDS             complement(18573..19205)
                     /gene="dhaL"
                     /locus_tag="ACHK01_002098"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO8097050.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19216..20286)
                     /gene="dhaK"
                     /locus_tag="ACHK01_002099"
     CDS             complement(19216..20286)
                     /gene="dhaK"
                     /locus_tag="ACHK01_002099"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO8097051.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSRVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20514..22433
                     /gene="dhaR"
                     /locus_tag="ACHK01_002100"
     CDS             20514..22433
                     /gene="dhaR"
                     /locus_tag="ACHK01_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO8097052.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22533..25399)
                     /gene="ycgV"
                     /locus_tag="ACHK01_002101"
                     /pseudo
     CDS             complement(22533..25399)
                     /gene="ycgV"
                     /locus_tag="ACHK01_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(26240..27331)
                     /gene="ychF"
                     /locus_tag="ACHK01_002102"
     CDS             complement(26240..27331)
                     /gene="ychF"
                     /locus_tag="ACHK01_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO8097053.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27448..28032)
                     /gene="pth"
                     /locus_tag="ACHK01_002103"
     CDS             complement(27448..28032)
                     /gene="pth"
                     /locus_tag="ACHK01_002103"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO8097054.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28310..28588
                     /gene="ychH"
                     /locus_tag="ACHK01_002104"
     CDS             28310..28588
                     /gene="ychH"
                     /locus_tag="ACHK01_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO8097055.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28643..30322)
                     /gene="dauA"
                     /locus_tag="ACHK01_002105"
     CDS             complement(28643..30322)
                     /gene="dauA"
                     /locus_tag="ACHK01_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO8097056.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30447..31394)
                     /gene="prs"
                     /locus_tag="ACHK01_002106"
     CDS             complement(30447..31394)
                     /gene="prs"
                     /locus_tag="ACHK01_002106"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO8097057.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31545..32396)
                     /gene="ispE"
                     /locus_tag="ACHK01_002107"
     CDS             complement(31545..32396)
                     /gene="ispE"
                     /locus_tag="ACHK01_002107"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO8097058.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32396..33019)
                     /gene="lolB"
                     /locus_tag="ACHK01_002108"
     CDS             complement(32396..33019)
                     /gene="lolB"
                     /locus_tag="ACHK01_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO8097059.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33233..34489
                     /gene="hemA"
                     /locus_tag="ACHK01_002109"
     CDS             33233..34489
                     /gene="hemA"
                     /locus_tag="ACHK01_002109"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO8097060.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34531..35613
                     /gene="prfA"
                     /locus_tag="ACHK01_002110"
     CDS             34531..35613
                     /gene="prfA"
                     /locus_tag="ACHK01_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO8097061.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35613..36446
                     /gene="prmC"
                     /locus_tag="ACHK01_002111"
     CDS             35613..36446
                     /gene="prmC"
                     /locus_tag="ACHK01_002111"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8097062.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     THLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36443..36835
                     /gene="sirB2"
                     /locus_tag="ACHK01_002112"
     CDS             36443..36835
                     /gene="sirB2"
                     /locus_tag="ACHK01_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO8097063.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36839..37648
                     /gene="sirB1"
                     /locus_tag="ACHK01_002113"
     CDS             36839..37648
                     /gene="sirB1"
                     /locus_tag="ACHK01_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO8097064.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37684..38538
                     /gene="kdsA"
                     /locus_tag="ACHK01_002114"
     CDS             37684..38538
                     /gene="kdsA"
                     /locus_tag="ACHK01_002114"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO8097065.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38687..38794)
                     /gene="ldrA"
                     /locus_tag="ACHK01_002115"
     CDS             complement(38687..38794)
                     /gene="ldrA"
                     /locus_tag="ACHK01_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8097066.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39222..>39315)
                     /locus_tag="ACHK01_002116"
     CDS             complement(39222..>39315)
                     /locus_tag="ACHK01_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8097067.1"
                     /translation="FAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         9745 a         9823 c        10117 g         9630 t
ORIGIN      
        1 tggcgaatgc ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag
       61 cgtcgtcgcc agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctatg
      121 cagcgacaaa tattgatagc ctgaatcagt attgatctgc tggcaagaac agactactgt
      181 atataaaaac agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc
      241 cgcgaaattg tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg
      301 gcagcagatt acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc
      361 gcgacttact tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt
      421 gatttactga ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct
      481 gttgacggcg agtttacggt gaaaaaattg caactacgcc cgacggtaca gcttattccc
      541 atgaacagtg cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt
      601 gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt
      661 tttatgccag ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc
      721 tatcgaataa tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta
      781 aaatgggcga tccctggttc aaacaaaaag atctgtttcg tcgctgtggc gtggtttgct
      841 ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag
      901 aactatcgcc ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg
      961 tgcgtaattg tcgcgatctg actgattttg gcagagaaat tcgcgcaacg gtgctacaac
     1021 gtacccatct tactgttggt gtggggatcg cccagaccaa aacgttggct aagcttgcca
     1081 atcatgcggc aaaaaaatgg cagcggcaga cgggtggggt ggtggattta tcaaatctgg
     1141 aacgccagcg taaattaatg tctgctctcc ccgtggatga cgtctggggg attggacggc
     1201 ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt tctcgatttg gcggatacag
     1261 atatccggtt tatccgtaaa cattttaatg tcgtgctcga aagaacggtg cgtgaactgc
     1321 gcggcgaacc ctgtttgcaa ctggaagagt ttgcaccgac gaagcaggaa attatctgtt
     1381 cccgctcgtt tggtgaacgc atcacggatt atccatcgat gcggcaggcc atttgtagtt
     1441 acgctgcccg ggcggcggaa aaacttcgca gcgagcatca atattgtcgg tttatctcca
     1501 catttattaa gacgtcacca tttgcgctca atgaacctta ttacggcaat agcgcgtcgg
     1561 taaaactgct gacgcccact caggacagca gggatatcat taacgctgct acgcgatctc
     1621 tggatgccat ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg ctgggggatt
     1681 tcttcagtca gggagtcgcg cagctcaatt tattcgatga caacgcaccg cgccccggga
     1741 gtgagcaatt gatgacggta atggatacac tgaatgctaa agagggcaga ggaacactct
     1801 attttgccgg gcaggggatc cagcaacaat ggcagatgaa gcgtgccatg ctttcaccac
     1861 gttatacaac gcgaagttct gatttactgc gggtcaaata aatatagcgg caggaaaaaa
     1921 gcgctcccgc aggagcgctg aagggattag cgaccgaaca ggtcacgttt tttcgcttta
     1981 aacggctggg aaatcaccac cagcaccgcg acaatcaggt aagcgataaa aataccgagc
     2041 agccactgcg gcatttccag acctaaaaaa tcccactgac gctcggcaca atcgccagag
     2101 gcgacaaaca cttgcggcac ccatttatcc agcggcagcc attccgggaa acgaaccata
     2161 aaatcacagg tggcaaacgg cgaaggatag agctgaagca tggtgtgctc gtaagttaac
     2221 tgcacaccgc ggaacgcact atacaaccag ataaccatcg ctacataacg cagcggagtt
     2281 ttcggggcga tcgcgccaat cagcgcagca cccagaacgc cgaataacgc acaacgttca
     2341 taaatacaga gcacgcaagg tttcagtaac atcacatgct ggaaccacag cgccgtcagt
     2401 tccagtgcca gagcagtaaa cgccatcaac agccacgcgc cccggccttg tgaacattgg
     2461 ttcaaaaatc gcaacataat catttccctg caatatgcat agagtgcgca gtttaaacca
     2521 attcattcgc tgcgccacca ggaggcagca aaaataggaa gcaattggac aattatcggg
     2581 cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg ttgctatcca
     2641 gcccatttgc ataaaccatt cggttacagg ggcaagcgta aactcgacgc agagcaagcc
     2701 gacgagtgtc agtacgaggg tgtaaggcag ggccatccac accatgcggc cataagagag
     2761 gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg aacgcagcct gaccgttcgg
     2821 cgtagcgacg gagggcagat tggtaccggt attaatggcg accgccagca gctcgtattg
     2881 cttcaacgtg atagcgccac tttccattgc cgctttcgct tcgttgatat aaatcgtccc
     2941 cacgaagacg ttatccgaaa tggatgacag cagaccgttg aaaatataga acagcgacag
     3001 ctgagcatgt tccgatgcct gcaacacaaa ctgaataatt ggcgaaaaca gttgttggtc
     3061 gataatcacc gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg gcagagattc
     3121 ggtgaaggct ttaccgatag catgttcatc ggtgacaccg gtcaatgatg tcgccagaat
     3181 aatgactgac aaaccaatca agccaacttc cgccagatgc aacgccagcg cagtcaccag
     3241 ccagacgcca ataatcgcct ggacaatcag acggatttta tcctgacggg tacgctggtg
     3301 gcggctttga tcgtcaaact gttgcaacac ttcgcggact ttctccggca gcgtttcacc
     3361 gtagccaaac caacgcagct tctctaccag caggcaggtt aacaggccac aaatcagaac
     3421 cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc cagccgcttt
     3481 agcgatgatc aggttctgtg gttcgcccac catggtcatc acgccgccta atgcagtgcc
     3541 gacaccggca tgcatcatca ggctacgcag aaagccacgg aactgttcca gaaccacttt
     3601 gtaatgcttg tcgatatgac tatcgtcttg caggtcggta tcttcggtac gggaagaggc
     3661 tacgcggtga taaataccat aaaaaccgac agcaacgctg atcaccaccg ccacgacggt
     3721 taaggcatcg aggaacgcgg agaggaacgc tgccgccacg caaaaagaga gcgacagcag
     3781 cattttggag cgaatgctta acagcaaacg ggtaaatatg aacagcaaca gctgtttcat
     3841 aaaatagata cccgccacca taaacatcaa taacagcaag acttcaagat ttgccgccac
     3901 ctcttcacgg acgtgttccg cgctggtcat gccgatgaat accgcttcga tagccaacag
     3961 accaccgggg agcagcgggt agcatttcag ggccatcgcc agagtgaaaa taaattccgc
     4021 taccagcaac cagcccgcga cgaaagggct gatgaggaaa attaacgggt ttacgattaa
     4081 gaaaatgatg agggcgagtt tgtaccagtc gggggactgg cccaaaaaat tgcgccatag
     4141 cgcgcggccc caggagatct ccatgatggt ttcccttacc ttacaaataa tcaatgatgt
     4201 ttttatgttt aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc ttgatagtca
     4261 aggggaaaga gatgcggaaa atgaagcctt gatccctttt ttcttctttt tgtctgctat
     4321 cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc
     4381 tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa
     4441 gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag
     4501 aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg
     4561 gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacctctg
     4621 gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg
     4681 ataatttgct gtcggtgcgt accaatattt ccactatttt tattcgcacc gcgtttcgtc
     4741 agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg
     4801 atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc
     4861 cgatttacgg tctgatcctt aacgggatga aagggctgta tacgcgcatt ggtcgtcact
     4921 atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt
     4981 tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga
     5041 gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc
     5101 gataatccct tccgtttaaa gagcaaaccc ctcaaacgag gggttttttg ttgtttttac
     5161 agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc
     5221 tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcagcac ttctttgcgc
     5281 ccccgatcca gcggtgcgcg attatgtgga atgtaacgca gcgtcagcga acggtcgccg
     5341 cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac
     5401 gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga
     5461 taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt
     5521 gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt
     5581 tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac
     5641 tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatatgg gttgatgccg
     5701 ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac
     5761 tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag
     5821 tgccagaagg tcgcccagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac
     5881 tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca
     5941 tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca
     6001 gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt
     6061 tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc
     6121 gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt
     6181 tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc
     6241 agataatcga caatcgaact ggcgtcggtc caactgcgga ataagtaatt gtttttaaag
     6301 aaagagttat gcccatagca ggcgtgtgcc atcaccagcg cctgcatggt aatggtgttc
     6361 tcttccatca ggtaagcgat acacgggtta gagttaatga cgatttcata ggccagtcct
     6421 tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc
     6481 ggatagttaa ttggcatgcc gacgctggag taggcatcca tcatctgttc tgaggttatc
     6541 acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct
     6601 gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg
     6661 tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg
     6721 tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt
     6781 ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat
     6841 gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag
     6901 ggagatgtga gccagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt
     6961 tatcaagatg taattagatt attattcttt tactgtatct accgttatcg gagtggctat
     7021 gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca
     7081 ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagcgc
     7141 cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcggcac caggtgtgcc
     7201 tttaaaagcg atcaaatgga tgttccagcg ccatgcgccg ctggcggtcc gtctcgacgg
     7261 tacgcagttt cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta
     7321 catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc
     7381 attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt
     7441 ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg
     7501 cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggagcccg cgctggcaga
     7561 agtagcgcac aaactgacgg gtggtctgca actgcccaat gatgaaaccg gagactgcca
     7621 gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccgctttaa
     7681 tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtgtggcga
     7741 tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct
     7801 caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga ctattccaat
     7861 tgcgcaggaa gatggtgcgc cagtatccac tattcttgat gaaacctaca aaatcgccat
     7921 tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac
     7981 cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg
     8041 cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg
     8101 tacgccggtt gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac
     8161 gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg
     8221 cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc
     8281 atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat
     8341 acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc
     8401 gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg
     8461 tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat
     8521 aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat ttttccatgc
     8581 tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca gcaactggca
     8641 gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa
     8701 cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg cttaccgtct ggcagcagtt
     8761 gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg cattttgccg aagcggaaca
     8821 tcctgatgga atttccagcg cgatggcgcg tattgagcag gcggcggaag ggctggagtg
     8881 tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc attttgactg
     8941 ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc
     9001 caataccgga ttacgtccgg tgatgacgct aagcagtgag attattggtg tccagacgct
     9061 aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg aacagcgaat
     9121 cggcattgtc gccgcagggt acgccgacgg ttatccgcgc cacgcgccta ccggtacccc
     9181 tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg atatgctggc
     9241 ggtcgattta acgccttgcc cgcaggcggg gattggtacg ccggttgagc tgtggggcaa
     9301 ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg agttgatgtg
     9361 cgcgctggcg ttacgcgtcc cggttgtgac agtgtaactt gttgtaagcc ggatcggaga
     9421 cagcgtcttc tggttgcgaa aaaatcatcc atccggctgg tcagcaactg tagttgttaa
     9481 tgtgacagag ccattgccca tgatagtgtc cattaaaaga atggacacta tttccccgga
     9541 acctgaactc accgcacagg cgttctacat aaaacgctta cgcttcattg ttgactcgaa
     9601 ctcgacttca gataaatcac gctttaccct tgatggagcc tgtacataga tttgtgtaat
     9661 tgcctgattt tgatatgttc aattcaacat caaatgaagg ttaaattatg gacgacaaac
     9721 aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc ggcgatagat gcttcattaa
     9781 atttaagatt cagcttcctc ttcagccacc cgtacgccaa tcttcaacac ttcattatct
     9841 tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt
     9901 gccgcgccta acaactgctg gacaatttca cccagcgttt gctgcttatc acgatattct
     9961 cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga
    10021 atgaagtcac caaagaagcg ttgatccagc gcgaccggcg gcgactggct gaacagttta
    10081 ccgagcgccg gaagatcgcg ttcccgacca attacacaca acacatcgcc ttcgcgcagt
    10141 cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagtgccgc aatacgcgtc
    10201 tcttttggca tatgcaaatc gcgcagtgcc gcgcccacgc accatttatc ggcactcaat
    10261 tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc
    10321 ggacgtccca ctggcggaac caccactttg gcttttttag ccgcccacga gagtgatgtt
    10381 ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagacgtgca
    10441 ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc gccgcgtaat
    10501 cctacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaggg gagcaatcca
    10561 gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga
    10621 atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg
    10681 atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc
    10741 ggccgattac ccagcagaaa accgcacaga taaacagcca gaatgccgct gccttccagc
    10801 gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg
    10861 gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagtccgatg
    10921 acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt
    10981 tcatgttgct ggatcatcgc aatcagggta atcgtcagaa agaccgccat tggatcatta
    11041 ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc
    11101 agagaaaaga ccgctgcagc atcggtagag ccgacgatag cgccgattaa taagccttca
    11161 atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagc
    11221 acgcccagcg tcgccagcga cagtgccggt cctaacgcca cacgaaagga actggcctga
    11281 gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca ggttacttac catgtaggcg
    11341 aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcctgccag catgccgatc
    11401 gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata
    11461 ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaac
    11521 ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga
    11581 cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct
    11641 aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag
    11701 cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg
    11761 ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt
    11821 gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata
    11881 gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt ccagcaccag
    11941 aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag
    12001 attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat
    12061 ggtgaaggtt ttattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc
    12121 atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc
    12181 cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg
    12241 ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta
    12301 accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag
    12361 atcttccagg cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc
    12421 gacgttattg acctgatgcc cctcgtcggt aaggcgatgg ataccacgca gcgcggcgtg
    12481 ctgtttaatg cagtaacccg atggggcaat taagtgaaac agagacatgg caattccttg
    12541 ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg
    12601 cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg
    12661 ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt
    12721 ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc
    12781 gtcgatccac aattgatcac ggcgattatc gctattgaat cgggtggtaa tccgaacgcg
    12841 gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat
    12901 gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag
    12961 cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc
    13021 attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt
    13081 gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg
    13141 gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc
    13201 tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg
    13261 ctttttctcg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg
    13321 tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc
    13381 catcaatcac tttgcgctcg ctgatggaga ttaactgggc gtcaaagtga aaaacacccc
    13441 attgccccat gttgacttca atctgagcga agcgcatgcc ttcttgtaat tcggcaggct
    13501 ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacagg cggaaacgta
    13561 acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga
    13621 tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtgataaatg
    13681 ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac
    13741 cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg
    13801 cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtaat ttgctgatca
    13861 gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca
    13921 cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga
    13981 aaaactcctg actgacaaac tgtctttggt ttatcggtca cagttaacaa aacttaatac
    14041 aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacatacgc ccagcatcga
    14101 tcccgccctt gcctatactt agagcgttga tgtaagcatt tcttgcgtcc gattcatcga
    14161 aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt
    14221 atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc
    14281 gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tggcggctcc
    14341 atcagtggtt ttaatctgca cagcttcctg gtggcggtgg tgggagctat tctcgttctg
    14401 ggcatattcc gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat
    14461 gccgctatcc gctttcacat cagaacgtat actcgacacc tgctttacgg gtgaaaaaaa
    14521 tcaatatagc acttcgatat ccgataaagt cggttcccgg taacaacaac agaatccttt
    14581 tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt agacagtagc
    14641 cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct tattgaacat
    14701 ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc aatgcttcag
    14761 ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct ggagtgtctg
    14821 gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag tgaacaccgt
    14881 aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa atcgccgggt
    14941 gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca cgtgtggcaa
    15001 acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa aaaaccatag
    15061 ctatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg tcggagtaag
    15121 cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg ctgacttaac
    15181 ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga tcaaatccag
    15241 cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat attcgccacc
    15301 gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt tctcccggtc
    15361 ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca cctctttttg
    15421 cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc
    15481 atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg
    15541 tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat tgacgtacag
    15601 cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca ggtcgtaatc
    15661 ggcataccag ccttgttgat cgttccacat gtatttttcg atcccttttt gacgggcatt
    15721 tgccagcgtt tcgtactggt ttgccatcgc gttatctccg gcagctttgc tggcgcgggc
    15781 gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga tgctggtggt
    15841 gcgtaaggta tttagttgat gcgggttgtc catccagcgc gagctgaaat cccagccaga
    15901 cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg gattgctttt
    15961 ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat cgtcccagta
    16021 gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt gtccggcttg
    16081 caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg gcaggtattg
    16141 cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg caaagaaggg
    16201 cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc
    16261 tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac cgctttcggc
    16321 aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg
    16381 cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt tttcggtaga
    16441 acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg
    16501 aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc
    16561 aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg gatcgctgtt
    16621 cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt gcacatcatt
    16681 aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt cttctgcctg
    16741 caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca ttttttgcgg
    16801 gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga ataaaacggt
    16861 attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg aaatctcatt
    16921 ctgcgaactg tcaggcattt tagaaaaggg aattaaaggg ataaataacg gcgaaataga
    16981 gtgatcaaat aacgtctgtt tattagccag ccagcggcgt tgggttcgtg attagcgaag
    17041 aaaaatcatc ataaattggt gaaaaatata acaggtagtt aaaaaccatt agtgctgagt
    17101 aaattgccgg atgacatcag aacgatgcca tccgaacagt ggcttaaccc tgacggttga
    17161 aacgttgcct tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca tacagtttct
    17221 caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg ctgtgggata
    17281 ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt tgcagtactg
    17341 tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc gggagttctt
    17401 ctttcgtttg ggtcaggtgg accagggtgc gatgcagaag atcgtccaca tcaatatagc
    17461 gagcttgtag atattcatca tccagttgct ggtattgctg gctaagttct ttaagaactt
    17521 gctgccaggc atattctgcc gtgcaatgtt catgctgaag gagttcgctt gccgccgcca
    17581 gcagttccgg atcatctaac agtgtatggt gaccagaaaa gattgcggca atatcgtcaa
    17641 gcccgctggc ttctgctttt gctgttaacg tcatcagatc taataacgtg aagtcaatag
    17701 cctggcgtaa tcgatcttgt tcttcttcca cggtcagggt tgattttgcc tgtaccgtac
    17761 ataaaactgg ttgataataa aaggctttac ccgaaacagg cggaacggga cgcagagtag
    17821 gtggagcgac ttcctccgtt tcaccaaagt tatcttcagc cagctgacgg aaagcgatca
    17881 gtgcctcttc agcttctggc cctttcgcaa tcaggcgcag cgtatcgtta tagcgaactt
    17941 gtagtaacgc aatctggtta atactctctg gtgtgacgca tttgccgttt ttttccagca
    18001 acatatcggc attaaatgtc gataaggtat aaaccagccg ggaggccgga cgtacatgca
    18061 ggccgttacg gttttttatg accaccgcca gagaacgggc ttcttcatcg tacgcaggac
    18121 atgtgtcaga gattttagtg tcggaggacg gtaaacccag ttgttcacgt ttggcttcca
    18181 gcgcatgcat agcgtcaaag ataactttgt cgatatccgc tcccgaggcc gcgctgaccg
    18241 ttgctgccag tgtaccttcg accaacggcg cagcacacaa acgtactttt gcggcgatct
    18301 tgggagccag caattccagc gcagtttcag cactcaataa tgcgctaccc atatccatca
    18361 tgaccagcac atggtcggca tcagcaacag attcgatggc ctccatcact ttgacggcat
    18421 cggtaccaat aggattttgt ggatcgtcaa ttcccgcggc aatggcgatt ttacaactat
    18481 cactcattaa catctgacgg gctaattcac cgacaccttc tcccagtcgg ctgctatgtg
    18541 aaactatgac caggtttacc atcaccaatt ccttactctt ttgcggctaa cgccaacatt
    18601 tgcatcataa acatcaccga ggtcgcgccg ggatcctggt gaccaatact gcgttcaccg
    18661 agataactgg cgcggccttt gcgggcttgc atcgtaatcg tactttgtgc agcggattcg
    18721 gcgattctac tggcagcttc gagcgccacc ggaacagaga gattttgctc gctggactga
    18781 cgtaacgatt ccaccaccgg cacccacaca tcacacatgg ttttatcgcc aggttcggct
    18841 ttcccgcgac tgattacgcc gtccacgcca tcgcggaaca tctgataaag ctcttccagt
    18901 gtcaggcttt gccgtgcctg ggtcgcctgt gcggcgcgga taaagaaggt accgaacagc
    18961 ggaccactgg caccaccgac gctggaaagc agcgtcatac cggtattctt gagaatgaaa
    19021 ccgatatctt tatctgcgat agcagggagt ttttccacca ctttgctaaa gcctcggttc
    19081 atatttagcc cgtggtcagc gtcgccaatt tcgcgatcca gtccggtaag atactcgctc
    19141 tcggtgctga aaatatcgcc acaacgagtg agccagttaa caatttgagt tctgctcagt
    19201 gacattgctt tctccttatt taccccagtt aagggccggg gtgtggaccg gggcgtccca
    19261 gagtgccagc gtttcgtcat caactttcag taaggtgatt gagaaaccgg tcatatccag
    19321 tgaggtgcag tacgcgccaa ttaaattacg ttcgatagtc aatcccgctt gctggcaacg
    19381 tgtggtcagg cggttataga cgccgtacag ctcagaaagc ggagttgcgc caagattgtt
    19441 aaccagcgca atcacccgat cgccagactg gagcggttgt ttggtttgtt gttcttcctg
    19501 ccaactgcct tgttgataat cccagaaacg caaagtgcga tggtatgagc cattttccag
    19561 cagggtgtcg aacatttcat cgacggtttg atcaagggaa gagaaggagc ggcggtcaat
    19621 acccggctca ccatgaatgc cgacgccaaa ctccatctca ttatccgcca gggtaaaaga
    19681 aggtttgccc gcggcaggaa cggtacaggc accgagagcg atacctattg agtggccttg
    19741 attattcaac ttacgcccca gttccgcaca ggcgtccagt gagtcgccac gctccgccgc
    19801 tgcgcctacg agtttttcaa ttaatacggt gttggcaacg ccgcgtcgcc cggcagtata
    19861 aagactgtct tttaccgcaa cgtcgtcatc aatgaccaca gtggtcactt ttacgcggct
    19921 atcgtgcagt aactcggtcg ctgtttcaaa gttaagaata tcgccggtgt aatttttgat
    19981 aatcatcagt acaccttcgc cgccatcaat ttgcatggcg cattcaaaga ttttatcggg
    20041 cgtcggtgag gtgaaaattt cgcccggaca ggcccccgaa agcatcccct gaccgatata
    20101 accacagtgc atcggctcgt gtccgctgcc gccacccgac agcagggcga cttttcctgc
    20161 aacgggggca tcagctcggg tgacatacac cggatcctga tgcagtgtca gagatggatg
    20221 cgctttcgcc agtcctgcca gttgttcgtc cagtacgtct tgcacatcat tgatcaattt
    20281 tttcattatt ttgctccagc aattacggta gggcatggat gatgttcaac gacacggcga
    20341 ccactgactg ccgatgaatc cattgtgcat caggcaaggg aaaagaaaat tagcccaaat
    20401 tatgtttcat agtgaaacat atgctttaat gaatgttcca tattgaaact tttacgtgta
    20461 ttaatactta aaattgcgag ccggaacacc ttttgtcata agggatgcgg gatatgagtg
    20521 gcgcttttaa caacgatggt cggggcatat ctcccttaat tgcaacctcc tgggagcgat
    20581 gcaataagct gatgaaacgg gagacatgga acgtaccaca tcaggcccag ggcgtgacat
    20641 ttgcttctat ttatcggcgt aagaaagcga tgctgacgct cggtcaggct gcgctggaag
    20701 atgcctggga atatatggca ccgcgagagt gtgcgctgct tatcctcgat gaaaccgcct
    20761 gcattctcag ccgtaatggc gatccgcaaa ccttgcagca gctaagtgca ctgggattca
    20821 atgacggcac gtattgcgcc gagggaatta ttggtacttg tgcgctatcg ttagcggcta
    20881 tctctggtca ggccgtgaaa acgatggccg atcaacattt caaacaggca ctctggaact
    20941 gggccttttg tgcaacgccg ttgtttgaca gcaagggccg attgacggga acaatagcgc
    21001 tggcgtgtcc ggttgagcaa actaccgcag ctgatttgcc gttgacgttg gcaatcgccc
    21061 gcgaggtcgg taatttactg ctgacggaca gtttgctcgc tgaaactaac cgtcatttaa
    21121 atcaacttaa tgccctgtta gaaagtatgg atgatggcgt gattagctgg gacgagcagg
    21181 gtaatttgca atttattaat gcccaggcgg cgcgggtctt gcgccttgac gcgacggcaa
    21241 gtcagggacg ggcaatcact gaactcttaa cgttacccgc cgtattgcaa caagcaataa
    21301 aacaggcaca tccgctcaaa cacgtagaag caacctttga aagccagcac cagtttattg
    21361 atgcggtgat aacccttaaa ccgataatag aaacgcaggg aaccagcttt attttgttgc
    21421 tccatcctgt ggaacagatg cggcagttga tgaccagtca attaggaaaa gtcagccata
    21481 cctttgctca tatgccacag gacgatccgc aaacccgccg cttgattcat tttggtcgcc
    21541 aggcggcgcg cagtagcttt cctgtcctgc tttgtggaga agagggcgtg ggcaaggcac
    21601 tgctaagtca ggcaattcat aatgaaagcg agcgtgctgc aggtccttat atcgccgtca
    21661 attgtgagtt atatggtgat gctgcgctgg cggaagaatt tattggtggc gatcgcacgg
    21721 acaatgaaaa tggccgtctg agtcggctgg aactggcaca cggcggcacg ctgtttcttg
    21781 aaaagattga atatctggcg gtggagttac agtctgcttt gcttcaggtt atcaagcagg
    21841 gggttatcac gcgactggat gcgcggcgtt taataccaat tgatgtcaaa gtgattgcaa
    21901 caacgaccgc ggacctcgca atgctggtgg aacaaaatcg ttttagtcgc cagctgtatt
    21961 acgcgctgca tgcatttgaa attaccatcc cgcctctgcg tatgcggcgt ggcagcattc
    22021 cggcgctggt gaataacaaa ttacgcagtc ttgaaaaacg cttctctacg cggctgaaaa
    22081 ttgatgacga tgccctcgct cgcctggttt cttgtgcatg gccaggcaac gattttgaac
    22141 tttacagcgt catcgagaat cttgctctga gtagtgataa cgggcgcatt cgcgtcagtg
    22201 atttacctga acatctgttt accgagcagg cgacagatga tgtcagcgcc acccgccttt
    22261 ccaccagtct gtcatttgcg gaagttgaaa aagaggcaat tattaacgca gcccaggtca
    22321 caggcggtcg cattcaggaa atgtcggctt tacttgggat cggccgcact acgctgtggc
    22381 ggaaaatgaa gcaacatggc attgatgcag ggcagtttaa gcgccgggta tgaaagacag
    22441 aaacgatttc tgatacatca gagtgatctg tatttcattc cggcgcacgc taacaatttt
    22501 cagcatcgtt taagggcttg tctatcccgc acttaaaagc tgaagcgata tcctccgttg
    22561 acttgcttct gatcaaagag gttaccctgc gtgtaatccg cttcgagata gaatgtgtgc
    22621 tgtttgttat actgtgcact gactcccacg ccgttattcc agccattacc tttgaaactg
    22681 tacttctccc gggagttgtt cacagatatt cggtatcccc tgaaaactcg cggatagcgc
    22741 cagtcttcac atagacattc agctggctgt tgcctgcggt gatgtcatac cccagtatca
    22801 tgctggcacg ccccagcagc gattcgtagt gattcagatg tatattgagg ccattactcg
    22861 ccttcatagc catctcattc tggtggctgt atgtaagctg ggtttgcggc tctatataga
    22921 acccataacc agtaggggac aggttgaacc tctgcccggc ttccagggag atgctcattc
    22981 cattcgcagt gccgttggcg ttaacgccgt tgttctgact gtccagtacg tggaaactat
    23041 ttttctggcg cgatgctttt ataacgagat cgctgtaaaa accgttttgt gccatgtaac
    23101 tggcgtacat tcccatgtag tctgaacgtg cggtaccgtc gcctccgcta tagtccggcg
    23161 atgcatgtgt tgagccaata tacagaccga catacaacgg cattacatca gagagacgtt
    23221 tatccccacc aaactggata ccgctgtaac ccatgtcaaa gccgctcagt ttgccactgg
    23281 caaaggagtc caggcttccc ccataactgc gcaaccagat attaccgtct ttactctgat
    23341 ttcgcaggtc acccatccgt tgcatcagcg tacggttttc aacatagttc aataagtaac
    23401 cgacattgag ataattgccg ccagcatctg cggtcgtggt gggtttaggc gtgggagcgg
    23461 gttcaggcgt aggatcgggg ttgactatgg gaggctgagc gggagctggt gtgggttcag
    23521 gattaggagt cggttccgga actgtcccgg aagcgtaaag ctcccagtta gtgccatttt
    23581 tacgcacatc gtacagatat ccccccaact caacctgaga agacgcgctg aacgaggccg
    23641 caccgtcagt ggtttttacc actgtcagaa cttcatttcc cgtagtggcc tcgctgccct
    23701 ggttgcggat agccaatacg tgattaccag cactacttcc gctgatattc aataaatccc
    23761 ctttattatt aacgccatta ccctcgccaa caacatcagc acgcataata aaggtactgt
    23821 taccgctcag gttctctacg tttaatgtgg caaatgtgcc ggcagttgac ccgtggctgg
    23881 caaaatcgac agttgaatgg ctcagcgcca gcgtgtcgag attagaatta cttgttacgt
    23941 tccagacgct gttattcatt gcaacgtcca gtttcccgcc attgacatta tcgctgaggg
    24001 aggaacctgt ccaaaccgac cctgggtgca tatctagatt gattagccca cctttggaaa
    24061 gaacgctacc gttgataagc atacgacccg aggcgttgat gcggctggcg gcataaccat
    24121 cgtcatgttg cgttgcaatg gccatctggt cgggtgtact catatcaatg accagatcgc
    24181 tcgtgaggtc gatctggctg ttggtcatgg cataaattcc cctggctcct gccgcgccgg
    24241 tgatagccaa actgtctccg gttattctac cgccgctgag cgcccacaaa cccagcgcca
    24301 gactgccatt acgatcaact gtaatatcgg tattttgcat gttgacaaca gccgttgccg
    24361 tctgggccga ggcaccataa gaaccgccgg aaaagatgct gtttcgttgc gctgccgtgc
    24421 cagaaaaatt gattgtcgcg tctgaaccac tggtgacgag gccaccgccc tgcgcggaag
    24481 aaatatggct atctgcaccg atagtggttg taccaccacg aacttcgacg ccattggccg
    24541 cggctccagt tacatcaaca gtgagtgcat tcgcgctaac ctggccaaag ctccagaggc
    24601 cgtgtgcatt atcgccattg gttttaatgg tactgtttgt tccgagatcg acaacagagt
    24661 ttttctgtac gtttatcccc atggcgctgt agccctgaac atcgattgtc aggtctgtcg
    24721 ccgtaaaacg cgcagcacca ttggcgttat tgccgttgag accaccgata taaacacctg
    24781 tacttccatc ggtcgtgatt ttacttccgc ttccaagatc gacactggtg ccatagtcat
    24841 tgatggttag gcctataccg ttcgagtttt caatggtgaa ttgagtggct gttagtgttg
    24901 agctatgccc aataatgatg ccgtcatcgt tactcttaat ggtgctgcct gtgcctaagt
    24961 cagcatgggt atagtcacca attaagttaa tgccgatggc agaggtttga ccaacaacat
    25021 ctactgtgag tcggttagct gtcagggagc tgtttttgcc ctgaataatg attccttttg
    25081 catatccaga ggcgtcgttg acattgacgg tgacatcatt acccaggttg atattgccag
    25141 gcgtgtttcc tgctggggtc attacaccat agaggtttcc tgatggatcg gcggtatctc
    25201 ctgtaattct gtcaccatca ttaatagtaa tattggtgcc atattgactt cctgtatcag
    25261 cagctatcag caggggggaa aagataattg cattcaaacc aatagcgcca aattttatca
    25321 gttgaattga gggcgaacgg atttttaatt cagcgagacg atctgctttg gtattcccat
    25381 tgtgttgttt gatgcccata ccactcctat atagtaccca gactatgggt ctatggattt
    25441 tgtcattttg tttaaatcaa tgaataatat cctcttatca tcatgatatt tgtatggtgc
    25501 aatcatgatg agcttctcag agaataattc tctattaccg ggcaaaatca ggaacacagg
    25561 aattaatata tatttaacat cccccttaca aaggtgggtt tatatcgagt tattccctaa
    25621 gttgtcgttg cttattccaa ttcgttacta taaatatggc aaaaatatta caacaaacag
    25681 cggttaaaaa atttggtgaa gattttttgt aataatccta attagttatg ggaatttcgc
    25741 ttgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
    25801 caaaaaacat cgatattcat ttttggtcaa tgggttttct ggcctatcgt tgttattgtg
    25861 ctactggtta tcattttcat cttgccagga tcgcacgtaa tcacgttatt cggggacttt
    25921 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc
    25981 aatattgatc agctgtgaag ggggtgatgt gtagtggacc aaaacttacg ttatatcact
    26041 actaatgaga tatccgtcat ctgacactga tgtctttaat tattctcgtg gttcactgta
    26101 ggcaataagc acaaaagtgt aggatgttac aagaatgatt aggactcggt gaaatgaaaa
    26161 atccacgcaa ttgcgtggat tatatatact tttgcgctct tcatgagatt tagcgaaacc
    26221 tcatgagaca acaaattaat tagacgttga aaaggaagtt catcacatcg ccatctttaa
    26281 cgatgtaatc tttaccttct gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt
    26341 tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg gataaagcct ttttcaaaat
    26401 cagtatggat tttgcccgct gcctgcggcg cggttgctcc aaccggaatg gtccatgcac
    26461 gcacttcttt caccccagcg gtgaagtaag tttgcaggtt cagcagctta taaccggcac
    26521 ggatcacacg gttcaggccc ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt
    26581 cttcgtcgtc cagttcggca atgtctgctt caacagcagc acaaaccgga accacaacag
    26641 aaccttcttt cgccgcgatt tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt
    26701 cttcgttgac gttggcgatg tacattgttg gtttcagcgt caggaagctc aggtaacgaa
    26761 tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag catacctgca ttttccaact
    26821 ggggcaggca tttttccagg accgccagct cagctttcgc gtctttatcg ccacctttgg
    26881 ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt
    26941 cggtgttgat aacttcaata tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt
    27001 tgtcattttc aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg atgttggtca
    27061 ggaactggtt acccagacct tcgcctttcg atgcgccttt taccagaccg gcgatatcga
    27121 caaattccat ggtcgtggga agcgtacgtt gcggttttac gatttcagcc agttgatcca
    27181 ggcgagggtc aggcattggt acgacgcctg tgttcggctc aatggtgcag aatggaaagt
    27241 tggccgcttc aataccggct ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg
    27301 gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata tcttaataat
    27361 caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt atacacggca
    27421 ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca atcggttcgt
    27481 tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc
    27541 ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac cgacaacttt
    27601 atttttatcg cccggatgac cgattccgat gcgtaaacgg tgaaagttag ggttattacc
    27661 caatttactg atgatgtctt tcagtccatt gtgaccacca tggccaccgc ccaatttaaa
    27721 tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt cgtccggatt
    27781 aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca taaatgtagt
    27841 cgggactaac aggcggacat cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa
    27901 tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc
    27961 accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa
    28021 tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt
    28081 gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcatgagt
    28141 ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg
    28201 ttgtaattgt gatcacgccc gcacataacc cacagggtgt tgtctatact ttacacataa
    28261 ggaagagggg tattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa
    28321 aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg
    28381 cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca
    28441 tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg
    28501 cgggcatgaa caggtgtgcg accgttactg gtgggttcgc cactatgaca aacgttgccg
    28561 ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg
    28621 cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga ttcgggaaga
    28681 acgccaggcg tcccgggatc ggttgaatgc cagcgcgagc catagtgcgc agtggctgga
    28741 attccacgtt gcacacgcgc agttcacatc cctcgggcag acgcttcaca aaacgctgga
    28801 acgcatcaag accaccagca tcaagtaccg gaaccgcatc ccacttcaga atcacaatcc
    28861 gtttgccttc aagacgtgac tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca
    28921 gcgggccaat aacgcgcaga accaggacat cgtctggaac atctacgact accggtgcca
    28981 ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc
    29041 tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc atgacaatga
    29101 tgtcatcttt cggcgcatga cgcagcaagt cgaccacttt gtgcgcttca ctcatgttcc
    29161 acgccaccat caacagcagg gcagccatgg cggaaagcgg cagccaggag agcagcggtg
    29221 ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgccgag ataggggaag
    29281 ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg gtaatgccac
    29341 caaagaacgg agcgatgata ttccccagtc cctgtccaac cagttcgcta ttcgccttgt
    29401 gtttcgtccc ggtcatacca tccagcacca cggcgcagag cagagattcg attgcgccga
    29461 gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag gttagcgtga
    29521 attctgaatt aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt
    29581 taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc
    29641 cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac
    29701 cgggtaaacg aatgcccaga cgcggccaaa aaacaagaat acctagcgtc acaatgccaa
    29761 tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta
    29821 gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac
    29881 cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa
    29941 tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa aatccccgac agcaaggtcg
    30001 caaccagcag tcctgccagt ccaaactgtt gcgacacggg atagagaatt accacaaatg
    30061 ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg acaatccccg
    30121 caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata gccaacgcca
    30181 tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc
    30241 gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat
    30301 gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta
    30361 aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc
    30421 gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagagat cgattcttcg
    30481 ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt acgcacgttc
    30541 ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac ttcatcaatt
    30601 acagagttac gcaggttatt cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca
    30661 aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag cgtaccgcca
    30721 gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc gatgatatgc
    30781 atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat atcggtatcg
    30841 ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca
    30901 attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct accaaatacg
    30961 ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc cactgtcagc
    31021 acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt gattggtaca
    31081 cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat aacagcggtg
    31141 atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc catcaggttg
    31201 tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg tacattttcg
    31261 ttgatttgta cactgacttc gccgtcgcta aagcgaccta cagcggcgtc gccgagtgaa
    31321 gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc agcaaaaagc
    31381 ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag aatctgccga
    31441 aaactgtgcg ggccaggcat gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg
    31501 tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag catggctctg
    31561 tgcaatgggg aaagattaac gcctttcgcc acaaagccat tgagccattc cggggcttgc
    31621 tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca ggcccctgtc
    31681 ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc aacctcgcga
    31741 aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag caacgtttct
    31801 attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac cggagtcgga
    31861 atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac cggcgttagt
    31921 atttcaccaa cgccttcggc aaacgcggca tgcccccgaa caaagacagg aacatctgcg
    31981 cccagcgtca gccccatttc cgccagctca tccatgctta gcccgcattg ccagagatga
    32041 tttaatgcca ccaggaccgt cgcggcattg gatgaaccac cgccgagacc gccgcccatc
    32101 ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcggaagacg cccgctgtct
    32161 gccgcagttt ttatcaacaa tcgcgctgcg cgaacgatca ggttatcttc atgttccacg
    32221 ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac gaagctcaat gctgatggtg
    32281 tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga
    32341 ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt ccgcattatt
    32401 tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg agttccatat
    32461 tggctggcat cgcaggttgc gttttggtgt cataaccacc ataaacaacc ttccagtttt
    32521 tgccattctg gctgtaggta atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg
    32581 ttgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga attggcattc
    32641 cggtcaattt gccaatcatc tcttcggcgt catcggcggt ataacgctga cctttattgt
    32701 cgactaactg cacgttaccc ggttgagcat tcagctccag ttccgtgctg cccaatgggt
    32761 tagtgagcag cagacggtag cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca
    32821 ctttttgttg gtcagaaata taagcgaacg cgccgcgagt ctgatactga ttaagattgc
    32881 gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg gcttttgcca ggacctttgg
    32941 gcgtggtaac ggaacaggca gtgagcacaa gagcagccag cggtagcagg cggataagac
    33001 gaaaatcggg caggggcata gtgatgacaa gtccttgaga tacgttgcag ttataaccct
    33061 taatgctagc gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt gccagaatct
    33121 aacggctttc ggcaattact ccaaaagggg gcgctctctt ttattgatct tacgcatcct
    33181 gtatgatgca agcagactaa ccctatcaac gttggtatta tttcccgcag acatgaccct
    33241 tttagcactc ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt
    33301 ttcgccggat aagctcgatc aggcgcttga cagcctgctt gcgcagccga tggtgcaggg
    33361 cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg aagagcagga
    33421 taacctgcaa gaggcgttaa tccgctggct ttgcgattat cacaatctta atgaagaaga
    33481 tctgcgtaaa agcctctact ggcatcagga taacgacgcg gttagccatt taatgcgtgt
    33541 tgccagcggc ctggattcac tggttcttgg ggagccgcag atcctcggtc aggttaaaaa
    33601 agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc gaactggaac gcatgttcca
    33661 gaaatctttc tctgtcgcga aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt
    33721 gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc tctctacggt
    33781 cacagtgttg ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga
    33841 acataaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc
    33901 agatgaagtt ggcgcggaag tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc
    33961 cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag gcatggtgga
    34021 gcgtgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg ccgttccgcg
    34081 cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagtg tggacgatct
    34141 gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc gcagcggttg aggcggaaac
    34201 tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga
    34261 aaccattcgc gagtatcgca gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc
    34321 gttagcggcc cttgagcagg gcggcgacgc gcaagccatt atgcaggatc tggcatggaa
    34381 actgactaac cgcttgatcc atgcgccaac gaaatcactt caacaggccg cccgtgacgg
    34441 ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc agtacatcat
    34501 tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc caaactggaa
    34561 gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca aactatcgcc
    34621 gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga tgtttcgcgc
    34681 tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat gatgctcgat
    34741 gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag
    34801 caactggaac agcaattaca ggttctgtta ctgccaaaag atcctgatga cgaacgtaac
    34861 gccttcctcg aagtccgagc cggaaccggc ggcgacgaag cggcgctgtt cgcaggcgat
    34921 ctgttccgta tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc
    34981 gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag cggtgatggt
    35041 gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg tgcagcgtgt tcctgctacg
    35101 gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc agaactgcct
    35161 gacgcagaac tgccggacat caacccagca gatttacgca ttgatacttt ccgctcgtca
    35221 ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac tcacttgccg
    35281 accgggattg ttgttgaatg tcaggatgaa cgttcacaac ataaaaacaa agcgaaggcg
    35341 ctttcagtgc tcggtgctcg catccacgct gctgaaatgg caaaacgcca acaggccgaa
    35401 gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac
    35461 aatttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta ccgcctggat
    35521 gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga acatcaggcc
    35581 gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg ttacgtgaag
    35641 caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc
    35701 atgttaccgg cagagggcgt acttttattc tcgcctttgg tgaaacgcat ctgactgacg
    35761 aacaatgtca gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa cccattgctc
    35821 atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca gcgaccttaa
    35881 ttccgcgccc ggatacggag tgtctggtgg agcaggcact ggcgcggttg cctgaacaac
    35941 cttgccgtat tcttgatctc gggacgggta ccggggcgat tgcgcttgcg ctggctagcg
    36001 agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc tgatgctgtc tccctggcac
    36061 aacgtaatgc ccagcatctg gcgatcaaaa atatccacat tctgcaaagc gactggttta
    36121 gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat attgacgagc
    36181 aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg
    36241 cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat
    36301 ccggcggctt tctgcttctg gaacatggct ggcagcaggg cgaagcggtg cgacaagcat
    36361 ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg
    36421 taacgctcgg ccgctattat caatgacaag tttttctaca ctgcttagtg ttcatcttat
    36481 tagtctcgcg ctttctgttg ggctattaac cttacgtttc tggctacgtt atcagaagca
    36541 tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt
    36601 gttaagcggc attgcgttga tggctaaagc gcacatcctg ccattttccg ggcaggcaca
    36661 gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt ttattgcact
    36721 cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc tggcgttggt
    36781 ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg ggtaagtcat
    36841 gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca tgatcctggc
    36901 ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac tggagcgtct
    36961 cgttagtctg gcgaaggaag aaatcagcca gcttctgcct ttagaagagc agctggaaaa
    37021 actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg tttatcgtct
    37081 ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg cggtatcatt
    37141 aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt
    37201 ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc
    37261 ttttaacggt gaatcgttaa gcgaacatat gctggacgta tggttaaagg gaaatatcag
    37321 cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg
    37381 caaattgctg gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt
    37441 acgcaccagc gaagctttat tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg
    37501 cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt
    37561 cgttgaacag tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat
    37621 cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat
    37681 cctatgaaac aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc
    37741 gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag
    37801 cactacgtaa ctgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac
    37861 aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa
    37921 atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca
    37981 agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct
    38041 cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa
    38101 ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc
    38161 aacgaaaaag tgattctttg cgatcgtggt gctaacttcg gctatgacaa cctggttgtc
    38221 gatatgctgg gcttcagcat tatgaagaaa gtgtctggta actcgccggt gattttcgac
    38281 gtgacccacg cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct
    38341 caggtggctg agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa
    38401 gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa
    38461 ctggaaccgt tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa
    38521 ctggatacca gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa
    38581 gacttttttc atgggtgcca gatacaaaaa aggccgcagg ctgttacccc tgcggccggt
    38641 ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac
    38701 gccaccagcc gacaatcgct gcggtaataa ttcccgccag gatcggtgct gccaggtcgt
    38761 gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
    38821 ttttgcggct attcttgaat tgttctggtt caagattagc ccccgttctg ttgtcaggtt
    38881 aaacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
    38941 ccccgcaaca ttgcgcctca ccggataacg ccggcttgat gtggatacta cgtctcaatt
    39001 catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt tttcaacagc
    39061 tgttacagtc gtttcatgag ggctctggat gatgcttcca gctcgggttg ccaatattta
    39121 cttgtggaag agataaagac aaaaatggcc gcaggctgtt acccctgcgg ccggtttcgg
    39181 gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg gttacgccac
    39241 cagccgacaa tcgctgcggt aataatcccc gccaggatcg gtgctgccag gtcgtgccag
    39301 aaaatcatgg caaac
//
LOCUS       DAZGCS010000040        38517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGCS010000040 DAZGCS010000000
VERSION     DAZGCS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.040"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..1489
                     /gene="dcuC"
                     /locus_tag="ACHK01_002117"
     CDS             122..1489
                     /gene="dcuC"
                     /locus_tag="ACHK01_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8097068.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1532..2029)
                     /gene="sspB"
                     /locus_tag="ACHK01_002118"
     CDS             complement(1532..2029)
                     /gene="sspB"
                     /locus_tag="ACHK01_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO8097069.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2035..2673)
                     /gene="sspA"
                     /locus_tag="ACHK01_002119"
     CDS             complement(2035..2673)
                     /gene="sspA"
                     /locus_tag="ACHK01_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO8097070.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3068..3460)
                     /gene="rpsI"
                     /locus_tag="ACHK01_002120"
     CDS             complement(3068..3460)
                     /gene="rpsI"
                     /locus_tag="ACHK01_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO8097071.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3476..3904)
                     /gene="rplM"
                     /locus_tag="ACHK01_002121"
     CDS             complement(3476..3904)
                     /gene="rplM"
                     /locus_tag="ACHK01_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO8097072.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4123..5250)
                     /gene="zapE"
                     /locus_tag="ACHK01_002122"
     CDS             complement(4123..5250)
                     /gene="zapE"
                     /locus_tag="ACHK01_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO8097073.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5444..5842
                     /gene="zapG"
                     /locus_tag="ACHK01_002123"
     CDS             5444..5842
                     /gene="zapG"
                     /locus_tag="ACHK01_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO8097074.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5996..7363
                     /gene="degQ"
                     /locus_tag="ACHK01_002124"
     CDS             5996..7363
                     /gene="degQ"
                     /locus_tag="ACHK01_002124"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO8097075.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7453..8520
                     /gene="degS"
                     /locus_tag="ACHK01_002125"
     CDS             7453..8520
                     /gene="degS"
                     /locus_tag="ACHK01_002125"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO8097076.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHK01_002126"
     CDS             complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHK01_002126"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8097077.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9955..10425
                     /gene="argR"
                     /locus_tag="ACHK01_002127"
     CDS             9955..10425
                     /gene="argR"
                     /locus_tag="ACHK01_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO8097078.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            10790..11053
                     /gene="yhcN"
                     /locus_tag="ACHK01_002128"
     CDS             10790..11053
                     /gene="yhcN"
                     /locus_tag="ACHK01_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO8097079.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(11108..11380)
                     /locus_tag="ACHK01_002129"
     CDS             complement(11108..11380)
                     /locus_tag="ACHK01_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO8097080.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(11472..13439)
                     /gene="aaeB"
                     /locus_tag="ACHK01_002130"
     CDS             complement(11472..13439)
                     /gene="aaeB"
                     /locus_tag="ACHK01_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO8097081.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(13445..14377)
                     /gene="aaeA"
                     /locus_tag="ACHK01_002131"
     CDS             complement(13445..14377)
                     /gene="aaeA"
                     /locus_tag="ACHK01_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO8097082.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(14385..14588)
                     /gene="aaeX"
                     /locus_tag="ACHK01_002132"
     CDS             complement(14385..14588)
                     /gene="aaeX"
                     /locus_tag="ACHK01_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO8097083.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            14771..15700
                     /gene="aaeR"
                     /locus_tag="ACHK01_002133"
     CDS             14771..15700
                     /gene="aaeR"
                     /locus_tag="ACHK01_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO8097084.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(15828..17273)
                     /gene="tldD"
                     /locus_tag="ACHK01_002134"
     CDS             complement(15828..17273)
                     /gene="tldD"
                     /locus_tag="ACHK01_002134"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO8097085.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(17429..21229)
                     /gene="yhdP"
                     /locus_tag="ACHK01_002135"
     CDS             complement(17429..21229)
                     /gene="yhdP"
                     /locus_tag="ACHK01_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO8097086.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(21297..22766)
                     /gene="rng"
                     /locus_tag="ACHK01_002136"
     CDS             complement(21297..22766)
                     /gene="rng"
                     /locus_tag="ACHK01_002136"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO8097087.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(22756..23349)
                     /gene="yhdE"
                     /locus_tag="ACHK01_002137"
     CDS             complement(22756..23349)
                     /gene="yhdE"
                     /locus_tag="ACHK01_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO8097088.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(23358..23846)
                     /gene="mreD"
                     /locus_tag="ACHK01_002138"
     CDS             complement(23358..23846)
                     /gene="mreD"
                     /locus_tag="ACHK01_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO8097089.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(23846..24949)
                     /gene="mreC"
                     /locus_tag="ACHK01_002139"
     CDS             complement(23846..24949)
                     /gene="mreC"
                     /locus_tag="ACHK01_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO8097090.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPVNTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(25015..26058)
                     /gene="mreB"
                     /locus_tag="ACHK01_002140"
     CDS             complement(25015..26058)
                     /gene="mreB"
                     /locus_tag="ACHK01_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO8097091.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(26363..28303)
                     /gene="csrD"
                     /locus_tag="ACHK01_002141"
     CDS             complement(26363..28303)
                     /gene="csrD"
                     /locus_tag="ACHK01_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO8097092.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEASSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            28455..29429
                     /locus_tag="ACHK01_002142"
     CDS             28455..29429
                     /locus_tag="ACHK01_002142"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO8097093.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            30407..30877
                     /gene="accB"
                     /locus_tag="ACHK01_002143"
     CDS             30407..30877
                     /gene="accB"
                     /locus_tag="ACHK01_002143"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO8097094.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            30888..32237
                     /gene="accC"
                     /locus_tag="ACHK01_002144"
     CDS             30888..32237
                     /gene="accC"
                     /locus_tag="ACHK01_002144"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO8097095.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            32346..32588
                     /locus_tag="ACHK01_002145"
     CDS             32346..32588
                     /locus_tag="ACHK01_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO8097096.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            32578..34029
                     /gene="panF"
                     /locus_tag="ACHK01_002146"
     CDS             32578..34029
                     /gene="panF"
                     /locus_tag="ACHK01_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO8097097.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            34041..34922
                     /gene="prmA"
                     /locus_tag="ACHK01_002147"
     CDS             34041..34922
                     /gene="prmA"
                     /locus_tag="ACHK01_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO8097098.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            35251..36216
                     /gene="dusB"
                     /locus_tag="ACHK01_002148"
     CDS             35251..36216
                     /gene="dusB"
                     /locus_tag="ACHK01_002148"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO8097099.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            36242..36538
                     /gene="fis"
                     /locus_tag="ACHK01_002149"
     CDS             36242..36538
                     /gene="fis"
                     /locus_tag="ACHK01_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO8097100.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            36624..37508
                     /gene="yhdJ"
                     /locus_tag="ACHK01_002150"
     CDS             36624..37508
                     /gene="yhdJ"
                     /locus_tag="ACHK01_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8097101.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            37592..37771
                     /locus_tag="ACHK01_002151"
     CDS             37592..37771
                     /locus_tag="ACHK01_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO8097102.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRI"
     gene            complement(37774..38436)
                     /gene="acrS"
                     /locus_tag="ACHK01_002152"
     CDS             complement(37774..38436)
                     /gene="acrS"
                     /locus_tag="ACHK01_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO8097103.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         9430 a        10097 c         9607 g         9383 t
ORIGIN      
        1 ctcacctaca attaaaaaca cgacatccgc accataaata gacttgcaaa aaatataaca
       61 tcattgtttt caatctgccg tttatgggat tgaccgtttt cttttgacac ggagttcaac
      121 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
      181 aaactacaaa cctcaggtgg tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt
      241 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      301 tatttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      361 tatggcggtg ggcggttatg cccgctacat ggagcgcaca ggagccagtc gcgcgatggt
      421 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      481 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggcc tgggtatgtt
      541 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      601 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      661 tgcagcccag gtcgcgggaa tgaaaattgc cacttacttc ttccactacc agcttccggt
      721 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      781 caaaaaagat aaaaatatca atcacgaaca ggcagagcta aaagctctcg ataatgtccc
      841 gccgctctat tacgccattt tacctgtgat gccgttaatc ttgatgctcg gctcgctgtt
      901 cctcgcccac atcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactgag
      961 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
     1021 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt
     1081 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1141 caggggtgct gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1201 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1261 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1321 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1381 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cgaacagcga tccccatggc
     1441 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag ccattaaaag
     1501 acaaaacagg ccaccagggc ctgtttttgt attacttcac aacgcgtaat gccggtcgac
     1561 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1621 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1681 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1741 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccgc
     1801 caaagcgcgc gttaaagcgt acctcatcat tcgccagttc cagattgccg acagcacgcg
     1861 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1921 ggagcgtcac atccaccacc aagtgcggcg tgagctggtt atccagcaac cactcataga
     1981 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     2041 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     2101 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2161 cagttgcggc agacgccaca gcagcggagc aagatagcaa tcgaccaggc tgaactcatc
     2221 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2281 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatggtgt tcatcagcgt
     2341 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2401 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2461 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2521 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2581 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2641 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2701 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccagta aggaacgact
     2761 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2821 atgatagttt accagatttt gcgacctttg tggtgagtcg atgccggaaa tggggaaaaa
     2881 gagatgcgct ttagtctgaa atagttgact tagtccctta ttggcgatgt ggtttttgtt
     2941 ttgcctgtct gtcagacggt agcaaaaagc aactttccaa tttttacgct gattcagaat
     3001 ttaaccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccggagcag
     3061 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3121 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3181 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3241 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3301 gttccagcgg ctgacgaact accatgcgag cagtttcacg accgaagtac tgttccagag
     3361 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3421 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3481 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3541 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3601 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtagcttgt ttgataccac
     3661 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3721 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtgtattccg
     3781 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3841 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3901 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3961 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     4021 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     4081 cggggtcaaa gatcgacccc tttttgtgat ttgtgacagg ttttaacccg ccaaatgctc
     4141 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4201 aaacttcagc cgatcgccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4261 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4321 catcaaccgc gtcataactg gtacatcaaa caacatgacc gtatgaaaga gacgtgagag
     4381 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag agaccgccag
     4441 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4501 attttctcgt ttcgcccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4561 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4621 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4681 acgcgcacgt tgcaggccat ttcgataaag ttcatccggc ggaatatttg acgtcgctac
     4741 cagggtaatg ccgcgggcga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4801 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4861 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4921 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4981 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     5041 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     5101 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5161 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5221 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5281 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5341 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5401 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5461 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5521 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5581 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5641 ggcgcacgac tatcgccagc tgtatcagca catggcgaaa agctccagca gcctgctgcc
     5701 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5761 cgatcaggca ccggtgcaga tgcctcgcga ctattctgaa ggtgcatccg gcctgctgcg
     5821 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5881 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5941 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     6001 aaaacaaacc cagctgttga gtgcattagc gttaagtgtc gggttaactc tctcggcgtc
     6061 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6121 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6181 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6241 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6301 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6361 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6421 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6481 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6541 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6601 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6661 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6721 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6781 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6841 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caggttctgg
     6901 ctcggcaaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6961 gaatagcttt gctgagttgc gctctcgtat cgcgaccacc gagccgggca cgaaagtgaa
     7021 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgctcg ataccagcac
     7081 ctcttcgtcg gccagcgctg aaatgatcac gccagcgctg gaaggtgcaa cgttgagcga
     7141 tggtcagcta aaagatggcg gcaaaggtat taaaatcgat gaagttgtca aaggaagccc
     7201 agctgctcag gctggcttgc aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt
     7261 gaactcgatt gctgaaatgc gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca
     7321 aattgtacgc ggcaatgaaa gcatctatct gctgatgcgt taatgtcgta aaccgggcat
     7381 caggcttacg tgtgatgtcc ggttaactcg tggtatgctg ctgccgttcc cttttttaat
     7441 gacgcctcca tcatgtttgt gaagctctta cgttccgttg cgattggatt aattgtcggc
     7501 gctattctgc tggttgccat gccttcgctg cgcagcctta acccgctttc cactccgcaa
     7561 tttgacagta ccgatgagac gcctgccagc tataatctgg cggttcgccg cgccgcgcca
     7621 gcggtggtta acgtttacaa ccgtggtttg aacaccaact ctcacaacca gcttgagatc
     7681 cgcaccttgg gatccggtgt aatcatggat caacgcggtt atatcatcac caataaacac
     7741 gtcatcaacg acgccgatca gatcatcgtc gccttacagg atggtcgtgt atttgaagca
     7801 ttgctggtgg gatctgactc tctaaccgat ctggcggtac ttaaaattaa tgccactggc
     7861 ggtttaccta ccattccaat taatgcacgt cgcgtaccgc acattggcga cgtagtactg
     7921 gcgatcggta acccgtacaa cctcgggcag accattaccc aggggattat tagtgccacg
     7981 ggtcgaatcg gtctgaaccc gaccgggcgg caaaacttcc tacaaaccga tgcttccatt
     8041 aaccacggta actctggcgg cgcgctggtg aactcgctgg gagaactgat gggcattaac
     8101 acgctgtcgt ttgataagag taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt
     8161 cctttccagt tagcaaccaa aattatggat aagctgatcc gcgatggtcg cgtgatccgc
     8221 ggctacattg gtatcggcgg acgcgagatc gcaccactgc acgcgcagag cggtggtata
     8281 gatcaactgc aagggatcgt ggttaatgaa gtgtcaccag acggcccagc ggcgaatgcg
     8341 ggtattcagg tcaacgatct gattatttcg gtggataaca aaccggccat ctctgctctg
     8401 gagacgatgg atcaggtggc ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg
     8461 cgtgatgata agcagttaac gctgcaggtc accattcagg aatatccggc aaccaattaa
     8521 gtcgagtgct caaaacaaaa accggagtct gtgctccggt tttttattat tcgctaatca
     8581 attacttatt aacgaactct tcgcccaggg cgatatcttt cttgagcgta tccagcatac
     8641 cttccagcgc gttctgttca aatgcgctca gggtaccgat agatttacgc tcttccacgc
     8701 cgtttttacc cagcagcagc ggttgagaga agaaacgggc gtactgaccg tcgccttcaa
     8761 cgtaggcaca ttcgacaacg ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa
     8821 aacgtgcagc tgcctggccc atagacaggg ttgcagaccc gccaccggct ttcgcttcaa
     8881 ccacctcagt acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa
     8941 aactaacgcc aggaacctgt gacagcagcg gcagaatggt aacaccagag tgaccgccaa
     9001 taaccggcac ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg
     9061 aacgaatgat atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt
     9121 ttttcagcac ttcagcagca atcgcaactg tggtgttaac cgggttagtg ataataccaa
     9181 tgcacgcttt cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt
     9241 taacgttaaa caggtcggaa cgatccatac ccggtttacg cgctacacct gcagagataa
     9301 gaacgacatc tgcgccttcc agcgccggag tcgcatcttc accagaaaaa cctttgattt
     9361 tcacagcagt agggatatgg ctcagatcga cagccacacc gggagtcact ggagcgatat
     9421 catacagaga gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct
     9481 ggccaatacc gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt
     9541 gataaactaa gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca
     9601 atgaccacat ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg
     9661 taatcaggaa cctaacttac gtaattaaca gtcgcttatg cattagcgca acattccaac
     9721 aggtggtgac aatataccct accgttcagc caaaacaaca tcaatttgat aacaattaat
     9781 ttacttttaa gcagaatttg catgccgtga cgcaggcatg tttctcaata acgaaatttg
     9841 ataaaatccc gctctttcat aacattattt cagccttctt cagggctaac tgtttgcata
     9901 aaaattcatc tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga
     9961 agctcggcta agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa
    10021 tttagctccc agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat
    10081 cagtctaaag tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa
    10141 atggaaatgg tttactgcct gccagctgaa ctgggtgtac caactacctc cagtccattg
    10201 aagaatctgg tgctggatat cgactacaac gatgcagttg tcgtgattca taccagccct
    10261 ggcgcggcgc agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg
    10321 ggcaccatcg ctggcgatga caccatcttt accacccctg ctaacggttt cacagtcaaa
    10381 gacctgtacg aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgt
    10441 ttctgacggc ggggaaaatg ttgcttatcc ctctcaatcc cctgctttcc cctgcgattc
    10501 atttaacgaa tagtgcgttt taccgcgaca tatcattcac gcaatgaata catgcggtaa
    10561 aaaaagcaca ttatgcaaaa ttcattatct gattgaaaag actggtattt ataataaata
    10621 accgtgtttt taatactttt ttgttattaa aattcacatt tttaacactt agtatcaact
    10681 gaaacagtta gcgtggtatt aattagttca ataattagtg tatacttgat tttgtgatat
    10741 gggtcactaa acaaaggccc agctaaaaga ttatgtcgag gtaaaaatca tgaaaatcaa
    10801 aaccactgta gctgcattaa gcgtactttc tgttctctct ttcggtgcat tcgctgccga
    10861 ctccattgat gctgcacaag cacaaaatcg tgaagcaatc gggaccgtat ccgtaagtgg
    10921 tgtggcgtct tcgccaatgg atatgcgtga aatgctgaac aaaaaagcgg aagagaaagg
    10981 cgcaacggcc taccagatta ctgaagctcg tagcggtgac acctggcacg ctacggctga
    11041 actgtacaaa taaaccctca tcgtcttgtc cgacgatatt gcccccggtt cgggggcttt
    11101 ttttgcgcta atgacgaaca ttaaaacgca aatgcccttc cagctcttcc tctgcctcat
    11161 caaacagcaa tattaacgcg ccaaaacggc gacgcgtttt ctctcccaga tgaacaaatt
    11221 caatctcaag tggtagcggc aggacatcgt tcattaacac atcccatagt gagtcgagat
    11281 cgcgtacctt atctttcgcc agaccaaagg tttggctaaa gtcacgataa aaatcctcct
    11341 gactctcaat ctcatcaaaa tcaaaggtat aaatattcat ctgttgccac cgtcacgttt
    11401 cgcggcgagt tctgccgtgc atcgctaagt atagccatga aaaaaccgac gcttttggcg
    11461 tcggttttga cttaactatc ggtcaacgca tgttgatact tatggagcat ccccgccagc
    11521 cgatgaaccg gttccgtcac ctgcggtggc gcttgccaga tgcgtagctt ttcctggtag
    11581 atttccagtt cttccagcaa ctggccaaag taccgacgac gtttatcatc gctacgggca
    11641 gatatcacat ggtctgctgt gcggcgcatt tgtcggtgaa acgccgataa atcctcgtta
    11701 accgggatcg gcgcatcacg caggcgctgg tgcgcgataa tcatcgtcag cgccaggcga
    11761 aatttcggca aatcccctgg gaacttattc atcagcaaaa acagctgctg ataaagtgcc
    11821 gggaggtggt tctctttacg acgtgccaca ttggtagtca tcgcggaaac agcggcagaa
    11881 acaaactgat taagcagtac acgtccagtc ctgtcgcgcg atttatcccg caccagcaaa
    11941 ataacggtga acgcgagcac acagccgacg atttgcccta atgcgctgtc gagaaactga
    12001 ctgaaatgga aagtcatcgg gttatccagc acgataatat ttatggtgct ggccagcgcc
    12061 cccatcgagc ccagtcgccg tttctgtact tctattccga ggaagaatcc cagcactgcc
    12121 aggctaatac acagcagcaa catgctctgt tgggtattag ggataatcac caaaaagtag
    12181 agcagcccta acggcagcgc ggccagcgtc ccgtagataa agtcgatcgc caccatgcgt
    12241 ggattcggca aacgcattgc cagtgacgtc acgaccgcaa tcatcaccat tgcaccactg
    12301 ccggaagtcc agcccgtcca cagccagaaa agcgttccca gaatgcagga aagtgtggtt
    12361 cgccagaagt taaccatcgc atgatgacgt tcggctgact ctactttgac ttccggttcg
    12421 ccttgcagga tctcttcttc ggtggcgttg atttttgtgt tactgataac gccgcgcttg
    12481 agaagctgat aacgcgttgc cgccgcgacc cagctataaa tggtgacagg cgtttcccgt
    12541 tccccggtcc aggcgataac tctccgcagg cgtttgagct gcttgtggac gtcctgcgcg
    12601 gtttctaccg gcgtgtcaaa aaattcgcgg aaagtatcag tgatcaattc cgggcgcgta
    12661 ttctgaataa gataagtttc gcaggattgg gtaatcagcg tcagcgatag cgtattgatc
    12721 gcttttaaac gtcgattggc ccgcgcccag cgggaagatt ccatattcag gttgctgcgc
    12781 atgccttgta gcgccgtggt gcgtcgcacc aggtcgcccc aggctttatc gacaacttca
    12841 ccatcgccat gcttgataca gagttgcatt aattgatatt gcgcgaccag caaactttcc
    12901 agctctcgat ccacttcttg tttgatcgat cgcggagaaa agagcaaatc cgccataatc
    12961 gcacacacaa taccgataac gatctcgcta caacgttcga cggcaaactg cggcgtaagc
    13021 aatggttccg gctgaatggt gatcacaatg atcagcgcgg tataaccggc cagcccccac
    13081 gcgtacgagt tttctattcg taccagcgag gatatccagg tacaaaaacc ggcccagata
    13141 cagcacacca gaatcatcaa taatggtgcg cggatcatcg caatgatgat caccagtccg
    13201 gcaatacagc caataaatgt gccgatgatg cgcaaaaagc cacgatagcg aatagcgcca
    13261 gaatacggtt cacctcccgc agcaaaggcc ggaccggcgg caacaatcgc cgctgtcagt
    13321 accgcccagc gtggcgtttc cagctggaag tgaaagccaa caaacagcgc cagtacaatg
    13381 gcggtcgcca gttttaccgc aaagcgaata tgttggttag caatggagaa aatacccatc
    13441 gtgattaacc aaactcacgc aggcgatggg ccattttacg gaagaacgaa tcctggcttt
    13501 cgtcgcgatc ttgtttgcca gtgaccacca ctgtagcagt ggtgcccgca ggccagatgt
    13561 tctcttgctg gttgtcgaga cgaatacgaa ccggaacacg ttgcgccaaa cgcacccatt
    13621 caaggttaga gtcgatggtc gccatccctt tgtcgtcacg cgtgctgctg gcgttggtga
    13681 ccccagcggc aacactatca acagtccctt tcagcacttt gttactgcca agcggcgtga
    13741 tctctgcacg ataccccgga cgcacccctt ccagcttagt ttcttccata taggccagta
    13801 catagaagga gttctgtttc accagcgcaa ccgccgttga tcctcgagta ataaactcac
    13861 cggtatagac gttgaggttg gtcacccagc catctgctgg cgcgcggatc accgtgcgtt
    13921 caagatccag ttttgccaga tcgcgggtcg cctgcgcttt cgctaactga tgcagaaccg
    13981 tttgtagtac gttgttagcc tggtcgatct cttcgcgaga catcgcctgc acaccgagac
    14041 ggttacgacg tccggcctcc tggcgtttct cctgtgccag tacctgataa taagcaacat
    14101 cggcttgcgc ttcctcaagc gccttttgat agcgcggctg gtcgatggtg aacagtatct
    14161 gtcctttttt caccagctgg ttatcatgaa cattcacctg ggtaatgagt ccagaaacgt
    14221 ccggcgcgat cgcaacgacg tcagcgctaa agcgcgcgtc acgcgtccag ggggattcgg
    14281 tgtaatagac ccaggcatta aaaattgcga tgaaggccag aatgactaat acgaccgtga
    14341 tggccgtacg ggagaatttt cttattagtg ttttcacttc aacctcaaac gaacagtcgc
    14401 gatatcaaat aaaacaagca gcaatagagc gcggtgttga acaacgccgg atgccagaca
    14461 aagtcgtaga tacctgttgg cacaagtacc cggcgcacca gccagaaaat cgccagtgat
    14521 aaaagcaatt caaaaaatat cggtgggaag gacagcccaa acaccacgat aacgggaaac
    14581 agactcatgt tgaccttggt tgtatagaga gagcaggcgt tattattttc agcatctgtc
    14641 gccgcagaga agggcatgga aagccgggcg agagcaacat tgctgtagat tgatatttaa
    14701 tatattagcg taactgttat gctgttatct atattatgtg atctaaatca cttttaagtc
    14761 agagtgaata atggaacgac taaaacgcat gtcggtgttt gccaaagtag ttgaatttgg
    14821 ctcttttacc gccgccgcca gacagctaca gatgagcgtt tcgtccatca gtcagacggt
    14881 gtcaaaactg gaagatgagt tgcaggtaaa gctgttaaac cgtagcacac gcagcattgg
    14941 cctgaccgaa gccggtagaa tttactacca gggctgccgt cgtatgcttc atgaagtgca
    15001 ggatgttcat gagcaactgt atgccttcaa taacaccccc atcgggacgc tacgcattgg
    15061 ctgttcttca actatggcac aaaatgttct cgccgggctg acagccaaaa tgctgaaaga
    15121 atacccaggt ttgagcgtca atctggttac cggaattcca gcccccgacc tgattgccga
    15181 cggtctggat gtggtgatcc gcgtcggcgc gttgcaggat tccagcctgt tttcccgccg
    15241 tcttggcgcg atgccaatgg tggtgtgcgc cgcgaaaagc tatctcacac aatacggcat
    15301 accggaaaaa cccgccgatt taagtagcca ttcatggctt gaatacagcg tgcggcccga
    15361 caatgaattt gaactgattg caccggaagg gatctcgact cgcctgatcc cgcaagggag
    15421 atttgtgact aatgatccga tgacgctggt gcgctggcta acggcgggtg ccgggatcgc
    15481 ctacgtgccg ctgatgtggg tgatcaacga gatcaatcgc ggtgagctgg agatcctgct
    15541 gccgcgttat cagtcagatc cacgcccggt ttatgcgtta tataccgaaa aagataagct
    15601 gccgctgaag gtacaggtcg tgatcaactc gctgacggat tattttgttg aggtcggtaa
    15661 attgtttcag gagatgcacg ggcgcgggaa agagaagtaa ttcatattgt actgttacgt
    15721 tctacaaacc tgtgccaacg ggttcccctc accctaaccc cagaggagcg aggggaccgt
    15781 tcgtgcacgt agaaagatta attatccttc tgaaaataat gaaattatta cgcagtaccg
    15841 ccaacagtca ggttatcaac tttcagcgtt ggctggccca cgccaaccgg caaactttgc
    15901 ccttctttac cgcagacacc cacgccgtta tccagtttca ggtcgttgcc aaccatcgaa
    15961 atctgctgca tggtttcgat accggaacca atcaacgtcg cgcctttcac cggcttcgtt
    16021 actttaccgt tttcaatcag atacgcttct gaagtggaga aaacgaattt gccggatgta
    16081 atatccacct gaccgccacc aaagttcggt gcatagatac cgtactcaac ggattcaata
    16141 atttcctgcg gggtcgattt acccggcagc atataggtgt tggtcatacg cggcatgggc
    16201 agatgggcgt aggattcacg gcgaccgttg ccagtcggcg tcatccccat caaacgcgcg
    16261 ttgagtttat cctgcatgta gcctttcaga atgccgttct caatcagcac gttgtactgg
    16321 cctggcgtac cttcgtcatc aatcgccacc gaaccacggc gatcgaccat cgtgccgtca
    16381 tcaaccacgg tacacagttc tgaagccacc agctccccga cctgcccact aaatactgaa
    16441 gtgccgcggc ggttgaagtc gccttccaga ccgtgaccta ccgcttcatg caacagcacg
    16501 cccggccaac ctgcgccaag tactaccggc atggtgcccg ctggtgcagc aacggcagaa
    16561 agattgacca gcgccatacg cactgcttct tttgcccatg catccgcacg gacttcgccg
    16621 tcaagatcgg caaggaagaa ctcataacca aaacgaccgc cgccgccact ggcaccgcgt
    16681 tcgcgtttgc catcttcttc gaccagaacg ctcacggaaa gacgcaccag cggacgaaca
    16741 tccgccgcca gcgtgccgtc ggtggccgca accagaatta attcataaac gccgctgagg
    16801 ctggcagtca cttcttgtac gcgcttgtcc gcttcgcggg caaccttatc gacgcgacgc
    16861 aggatatcca gcttctcttc acggctcatg ctttgcagcg gatctaccga ggtatacaac
    16921 gggctatgct ctaccgcgcc cagcgtctgt actttgccat caccactatc acggacgatg
    16981 gtgcgcgccg cttgcgcact ctgttccagc gccagcaggc tgatttggtc agcgtaagca
    17041 aatccggttt tttcaccact gattgcacgc acaccaacgc cctgatcgat gttgtaagaa
    17101 ccatctttaa taatgcggtc ttctaaaacc caggattcgt gatagctcga ctgaaaatag
    17161 agatcgccat aatcaaggcg acgttcggcc agttgaccga ggatcgcgaa caagtcctga
    17221 tgtttcaggc cgttcgccgc tagcaattgt tcacttacca ggttcagact catcgttttt
    17281 gctactcgtt agttactgca gtagaggatt ttttacggct gccggatgcg gcgtgaacgc
    17341 cctatccggc ctacagttat gttccggctc gttggccttt ggcaacgatt atcatatgag
    17401 attggggcaa ttacgcgccc tcgtcaaatc attgcgcttt ttctttacgc ggttggcgca
    17461 acacttcgtt gatttgcgga tcgtccagcg gacccgaaat gtgatagcgc aaaatggaga
    17521 ctttgctcca cagcggcccc agcactttac tggctgcaaa tactgccgcg ccaacaatgg
    17581 ggttaaccgc aaaagccgca gccacgccca ccgtcgcaga aatctctggt gcgacaaccg
    17641 cttccatatt caggtcgcga cgtaccagat ttaccgaccc tttcatggcg atatccgcct
    17701 ccaggccatc caccagcgtg tcgtcggtgt gcataacccc gtctttaatc cacgcggtgc
    17761 tgcgaatgga gtcaaaatag aacccttcgc caaaagtgtc tctgaaatca aaacgcagct
    17821 tacgcatcag ggcatcaacg ctcaataagc gcagcaattg cccggcatgt ccggtattga
    17881 tttcggtaat ttcgccttta cccagttgag tatgaatgat gccattcaac gtcgcctcat
    17941 ctggttgcca gggtgctttg cgccagtgta aatcgtaatc cacattaaat gacgactggc
    18001 gtatgggcgt cgtgacacca aaaaattctg cggcggcatc aattttctgc ccgcgcagtt
    18061 ttcctttcag cgaggtacgt tcattctccg gattattaac ccattcacca tcggcagtaa
    18121 gccgcgagaa accagtatca atcagtccat tggtcagcgt taacgtatcg ccagaaatgg
    18181 tgatatcact gtcaatgcga ccgaattttt gcccccagaa ccagcactct gtgcatcgta
    18241 tttgtgcgtc cggccagcca cggaagttaa tgcgctccgt tgtcgggaaa ggtgatgacg
    18301 gcgttgaatc accacgagtt ttcgccacgc tcgggttgta ataaagatat ttgatattcg
    18361 ccagccacgg cgcgttatta cgcatcgcta gcgtggcgtt gatttcacgc ccttgcgcct
    18421 caaccagggt gccatttgcc gtcggttgcg aaacaatact caggttattc cattgctgat
    18481 ttcccagtga caacataggc gtacgtaacg ttatgtgttg tgggaaactc gctgcaccac
    18541 cgacactctc cgcagcgcct ttctggaaca gggccagcca ctcggcacca ttcatcggcg
    18601 gcatattgag ttcaacgcca ctttgttccg gcaacggcgg gagcgtttta ctgtctgccg
    18661 cccaaatagc acgatcgagc gtcagctttt gaccaagcaa ccagcggcta ttgaaatggt
    18721 tatccgcacc agcctgtccg gttaattcaa agctgttgag attgccatca accttaacgt
    18781 ttaccgctag tggttcaccc gcaggtttgg ctaacggtga aggtaagtga ctactcacat
    18841 tattcagatc gccgttcatc tctacgttat aggtcgcgcc agcatgataa ggcagatcaa
    18901 tgcccacttt accatcccac gccacgctgc cactcaatgc ttcgttcacc gcttcaggca
    18961 gaacgccggt tttcgccggt tgccagttac cgttgaggtt taccgctacc tggtaggctt
    19021 ttgccccttc tttggtggaa aaatccacgt tcaacggctg attaaaccag cttgctgtca
    19081 gtggttcact ttgcagatcg ccattgataa agctgaattt accgctcaaa tttttcaggg
    19141 tgctgtcgag tggtttgata aacagactgt tattacgcag cgtcacttca cctttcgcgg
    19201 ttaccaactc gccgttcagc gggatatcaa gatgtaagcg agcattcaca tcgccgtcga
    19261 gctggagttc ttgcagggtc gcacccaggg aatctttcag cggtgtctca tcaaagtaag
    19321 ggcctacggc tttacccgga cctttaatgt cagcgtcaat cagcagtttt tcttttgagt
    19381 agtcagggat cactgcggtg agattactcg cgcgcacgcc gcccagatta acgccatcgg
    19441 ttttcatcca taaaccgtcg ttaataaagt ccagttcaat atcaaggtta gttaatgcag
    19501 gccagtccgg ctggaaggca aacttcgcgt tgcgcagcgg caccagcact tcaaactgac
    19561 cttcgttgtg tttatagggg aagagttgcg gattgccacc ataaaccagc gtcgcgttat
    19621 ccgcttcacc gccctgaatc gcgccactta agtaatcaac caggtcttta cccatcaagt
    19681 tttccgggaa atagcgccag gcttgtgaac catcatcggt actgatgcca gccagaatac
    19741 ccagccaggg ttcatcgtta gcaggttgca ggtaacgaaa accgccgcgc gcatggacgg
    19801 ctttggcttt aacgtcaata ttacgcccat ccagctggaa acctttgtca ttgttcagcc
    19861 agcttatagt tgcctggccg tcggcgattt ccagtggcgc acggaatacc gtttcgtaag
    19921 gcatctttgc ctgcttcatc gacgcggtaa gcaaaccatt ttcaacgctg ccggaaagcg
    19981 tcccggagaa gtgttccgca cccggtaata atttccattg cttccaggcc agatcgctcc
    20041 acgatgcctg aaaacgggta ttgtctgccg cctgaagcgg gatatccagc gccagagtgt
    20101 taatcttgcc gctcggttgt gtggagcgcc aaacatcacc cagtgcaggt gaaagtttcg
    20161 cggccagcgg gcgtatgccc tccaggcctg ccagctccag attactggcg cgaatccgga
    20221 gttcgtcact gcgtttattg tctttgccgc caacgtcctg ttccggtatc caggccagcg
    20281 tcaatgctcc gctcggccag ggtttaccgt ccatcgtgat ccgtgtatcg ggaatagaga
    20341 actgccagcc cggattttca cgcgtaatat gcgcggtcag attatccacc gacagcgtat
    20401 gcgtttcctt ctcgcccaac cagctggcac cgccctgttt cagccagacg tcaccgccgg
    20461 ttacatcgcc tttgtcgatc gtcatccagc cttcaaggga gaactgtgcc gtttccagag
    20521 caatattgtc ctgcatccat ttaccgagcc acggcttcag gtcgatgtca tccgcctgta
    20581 gccagacgcg accattgctt aacaacccct catcatcgcg caaatccatg cgcacctgca
    20641 tcacgccgtg ctgtccggta aggctggaga ggcttaccag gccttccgca cggtgtcgac
    20701 gtggatcgtt cagccaggtg agttgtggga tcgccagctc ggcgcgctga ccggatggcg
    20761 tcagaaaact gacttcactg tcgcggagat cgaaatggtc aaattgacga agaaacagat
    20821 cgctgatgtg actggcttcc agactgtcat cgctaccacc gctggtgata ggagtgttgg
    20881 tgcgaaagcg cagctgccag aaagtgaggt cgcgaaactg ccagcgcata tgtaacaggc
    20941 tctgccagac atccagcgcc agagtaacgc gtttaaccga aaattcgccg ccatctttta
    21001 gttctgcacg gatgtcgtgt gcttcaagcg tcgggccaaa attctgccag ctggctgaga
    21061 gctgactggc ttctaccggc atgccagtcg cggattctat tttgttgagg atttccggac
    21121 gccaggcgtc aagatgcggt aaagcaatac gcaggccgct aaccagcagg gctgcgatca
    21181 caacgagcgc ggctccagta agcagtaaaa tccccggcaa tcgcctcacc cgtcactcct
    21241 tgtctgctaa aaatgtgact caaaaaccct ttgccggatg gcggcccagc atctgtttac
    21301 atcattacga cgtcaaactg ctcctggtta tagagcggtt caatttgtac tttaacctgt
    21361 ttaccaacga aaatttccac ttccgccagc gagtgtgact cttcgccttt caaggcttca
    21421 gctactgccg gagaagcata gaccaggaaa cggtcggagt cgtaagcatg gtggacacga
    21481 acaatctcgc gcatgatttc atagcatacc gtttccacgg ttttcaccgt tccgcgaccg
    21541 tggcaggttg ggcattcgtt acacagtacg tgctcaatgc tttcgcgggt gcgtttacgc
    21601 gtcatctcca ccagccccag cgccgaaaaa ccattaacgc tggttttcac ccggtctttg
    21661 ctcaacgcct gctccagcga gtgcagcacg cggcggcggt gatcttcatt attcatatcg
    21721 atgaaatcaa taatgataat cccacccaga ttacgcaacc gtaactggcg agcgatagcc
    21781 tgcgtcgctt caatattggt attgaaaatg gtgtcgtcca gattgcgatg accgacaaac
    21841 gctccggtat tgatgtccac ggtggtcatc gcttcggtct ggtcgataat cagataacca
    21901 ccggatttca gttctacttt gcgttccagc gctcgctgga tttcgttttc gacatcaaag
    21961 agatcgaaaa tcggctggcg tccggtgtaa tgctccagct tgctggtcat ctcgggaatg
    22021 tactccgagg taaactccag caatgcttcg taagtcaggc gtgagtcaac gcgaatgcgg
    22081 tccagttcgg cgtcggcgaa atcacgcaga acacgctgcg ccagcgccag ttcgccgtac
    22141 agctgataac gggtctgcgg gcgtttttta cgctccataa ctttggtcca gacgcgtttc
    22201 agataagcgg catcggaggc cagttcagcc tcgccaaccc cttccgctgc ggtacggatg
    22261 ataaacccgc cctgctcgtc gcaatactct gcgaccactt ttttcaggcg ttcacgttct
    22321 gattcgcttt caatacgttg ggaaacacca acgtgagaag cccctggcat aaagaccaga
    22381 tagcgagaag gcagcgtgat atcggtggtc aggcgcgccc ccttagtgcc aagcggatct
    22441 ttcaccacct gcaccatcag atcttgcccc tgacgaacca gttccgagat gtcgcgcacc
    22501 gtgaattgct tttgttcttc acccgccaca cattcggtgt gcggcatgat atcggatgca
    22561 tgaagaaacg cggctttatc cagcccaata tctacaaaag ccgcctgcat acccggaagt
    22621 acacgactta cacgaccctt gtagatattg cctactattc cgcgtcgcgc ctcacgttca
    22681 atatgaattt cctgcagaat accgccatca atatacgcca ctcgcgtttc cgaaggcgtt
    22741 acgtttacta acaattcagc cgtcatgttt atcccttttc tcacgcagtg cgttaaaatt
    22801 acttaataat tcatacgttt caaccagcgg taagccgact acggcgtgat agctgccatt
    22861 tatcttcctg acaaaacagc cacccagccc ctgaataccg tatgcacctg ctttatctaa
    22921 cggttcaccg ctggcgacat agcccgcgat gtcttcgtct gttaacgttc tgaaagtcac
    22981 atcggtgacc accaggcaat cgagaatgtg ctggctgtcg gccaacgcca ctgccgtcat
    23041 cacctgatgg gtctgacccg ataatttgcg caacatctgc gccgcatgct ctgcgtcgcg
    23101 cggtttctcc agcacttctc cattcaggat aacgatagta tccgcaccca gcaccgggag
    23161 atcctgcgcc gtttgcgcga cacccgcctg tgctttctcg cgcgccagac gcacaacata
    23221 ctgctgcgcg ctctcctgcg gctgacgctg ctcctcaatg cccgtaacaa tacgttcaaa
    23281 ggtcacgcca agttgcgcaa gtaactcctg acgacgcgga gaaccggaag ctaaatacag
    23341 agaagtcata gaaaccttta ttgcactgca aactgctgac ggactttacg catcagcaag
    23401 aaaatccacg gccagagcac cccattgact acactactcc agaacacttc cggtctgaaa
    23461 gagacgttaa tcactaaaaa ctctgcccag aaaacaataa tatccaccac cagcgaaagc
    23521 aacatgacga ccagcgcctg ctgccataat gcgaggttgc ggaaaagctg gtatttcagc
    23581 gccaccaggt aagcaatgat gctcatcgcc aatacgcgta cgccaagcgt cgagccgctg
    23641 atcagatcca gtatggcacc catcacaaaa cctgtgccca catttacgcg atgaggcaag
    23701 gccaggatcc aatacaacaa gatgagtaac acccagtttg gccggaaaac aatcaggtta
    23761 tccggccagg gcatgatttg cagcaacagc gcaatgagga aagagagcca gattacccaa
    23821 cgtccctggc tacgatagct cgccactatt gccctcccgg cgcacgcgca ggcggttgag
    23881 caccactttg cggcggcgta gccctttgtg gagagcggtt agcagcaggc tgtgtcggcg
    23941 cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc tgagcgatcc
    24001 ccgttgccgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat acctgcggca
    24061 tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc atcgggttag
    24121 cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc agccctgctg
    24181 tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg gaagagacaa
    24241 ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaagtcacc agcacatcac
    24301 caacacgaat atccgtattc actggtagat gctcaagctg caaatcatcc gtacaaccgt
    24361 taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc gcgtgggtcg
    24421 catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc tgaccaacaa
    24481 cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta cctttatcga
    24541 taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc accattttct
    24601 gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc tcctgtttgt
    24661 attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt aacgcccggt
    24721 tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc agcaattcac
    24781 gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa gtacggattt
    24841 gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc agcaccgcca
    24901 gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata agttatgcgt
    24961 attctcgtat cagaccaggc agggtaaaca gacatttccc ctgcctgcat ccgattactc
    25021 ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc cgccaccgcg
    25081 cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt cttccattaa
    25141 caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc cgcgctcgga
    25201 gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg cgctcacaat
    25261 accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg taaaaccgcg
    25321 tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt catcgcccgg
    25381 gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga tcagagaacc
    25441 gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac caatgcgcac
    25501 ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg taccaccacc
    25561 gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac caattgcggc
    25621 agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg cggattcacg
    25681 aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa cgcgcgggct
    25741 tggacgcata aagctgttgc tgtgcacttg tttgatgaag tgctggagca ttttttcagt
    25801 cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa tattgcccgg
    25861 cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt tcggtgaacc
    25921 ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc cttgtccttt
    25981 tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg aaaacatgcc
    26041 acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa aagaaaatcc
    26101 gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg ctacggtgaa
    26161 aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa aggcttaatg
    26221 catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg catatgtgct
    26281 gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg ttattctacg
    26341 tgaaaacaga ttaaacggca ggttaaaccg agtatctttg tgaatatttt ttcacgttag
    26401 tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca ccgcgctgaa
    26461 tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacttgg gtgctggtcc
    26521 cggagctggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc ttctcaatgt
    26581 ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg atccaactgg
    26641 tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa gcattcacta
    26701 aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct gcaagttcaa
    26761 taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc agccaacgct
    26821 gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt tcctctggcc
    26881 agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct tccgataagc
    26941 caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta ccatcgaaga
    27001 tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc ggtttttgat
    27061 aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag cgaacattac
    27121 cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg ccctgcaatc
    27181 ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc tgaccactac
    27241 gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta ttgttcggca
    27301 aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt aacgtccggt
    27361 gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg ccagggtagc
    27421 gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca acctggctgt
    27481 gcccccaggt atcgctcaac atattgaaat ccggcagacg aatcatcatc acgatcccgt
    27541 gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg aaaaagagtc
    27601 ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc gtatcaagac
    27661 ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatctagc gcactgctgg
    27721 ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca ccgtttaaga
    27781 tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt aaccagcgta
    27841 ccgccaggaa gagcataaca atgataaagc caatcgcccc cgtgagcggc gcggtagtca
    27901 ttaaagaatg gaaatagttg cccatcggat cctgataaac cagacgcagc gacatccccg
    27961 gatgctttat caacggaaca ctcagttcgc gaaacagatc gctggagcca accggacgat
    28021 aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg tctacacgaa
    28081 cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg aagtcattcg
    28141 acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta aacttatact
    28201 gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt gttaacccgg
    28261 tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg ttaactccga
    28321 tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt gcgcttcatc
    28381 ggctttgctt ttccattagc gagtatagtc ttcagaaatt attttccaat ccatcatgca
    28441 catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa actctcgcat
    28501 cggtacagac acttgacgaa agtcgcctgc cggagggcga tgtcacggtt gatgttcact
    28561 ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa atcatccgta
    28621 attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc gaagatccgc
    28681 gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa aaccactggg
    28741 gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg ccgcaagggc
    28801 tggacgcgcg taaagcaatg attatcggta ctgccggttt taccgccatg ctgtgtgtga
    28861 tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg acgggtgcca
    28921 gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat caggtcgttg
    28981 ccgtttccgg tcgcgaaagt acccatgaat acctgaaaag tttaggtgcc agtcgtattc
    29041 tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg gctggggcaa
    29101 ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat tacggcggct
    29161 gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc atgccattta
    29221 ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca gaacgccgcg
    29281 cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc caggcggcaa
    29341 aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat aaccagatcc
    29401 agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa gagagggaac
    29461 tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg acagctttcg
    29521 tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc atgatttcga
    29581 ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga tatgcctccg
    29641 tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct attactgatt
    29701 tctggtccca tggggtgaat aatctgattt tatttgacta caaattaacc actcgaacct
    29761 atttaatgct gagcattgtc agtcggttga ttttgcgtgc tttagcattc acatctatcc
    29821 agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc gtctatcttg
    29881 tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca ttgcagcccc
    29941 tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag ttccgtcgat
    30001 gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt tgaaggttat
    30061 ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg ccaattgcta
    30121 cgaaatcgtt ataatgtgcg acctcgtcct ccctgacaca gtttttgcgc tgcggaaaag
    30181 gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca gcagatcgct
    30241 gcacaatgtc cgtcaagtct aacattgaca ctctggggca aaatagaccg gcgtcccggc
    30301 ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca gacggcggtg
    30361 aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg atattcgtaa
    30421 gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg aaatctccga
    30481 aggcgaagag tcagtacgca tcagccgtgc agctcctgcc gcaagtttcc ctgtgatgca
    30541 acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag ccgctccggc
    30601 gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca tcgtacgttc
    30661 cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt tcatcgaagt
    30721 gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga aaatgatgaa
    30781 ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa gtggacaacc
    30841 ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg ctggataaaa
    30901 ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt aaagaactgg
    30961 gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac gtattactgg
    31021 cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg aacatcccgg
    31081 caatcataag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt tacggcttcc
    31141 tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc ttcattggcc
    31201 cgaaagcaga cactattcgc ctgatgggtg acaaagtgtc ggcaatcgcg gcgatgaaaa
    31261 aagcgggcgt tccttgcgta ccaggttctg acggcccgct gggcgacgat atggataaaa
    31321 accgtgccat tgctaaacgc ataggttatc cggtgattat caaagcctcc ggcggcggcg
    31381 gcggtcgtgg tatgcgtgta gtgcgcggcg acgctgaact ggcacaatcc atctccatga
    31441 cccgtgcgga agcgaaagcc gcattcaata acgatatggt ttacatggag aaatacctgg
    31501 aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac gctatctatc
    31561 tggcggaacg tgactgctct atgcagcgtc gtcaccagaa agtggtcgaa gaagcaccag
    31621 caccgggaat tacaccggaa ctgcgtcgct acatcggcga acgttgtgct aaagcgtgtg
    31681 ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac ggcgagttct
    31741 atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa atgatcaccg
    31801 gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg tcgatcaagc
    31861 aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc gaagatccga
    31921 acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc ggttttggag
    31981 tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat gactcaatga
    32041 tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc atgaagaatg
    32101 cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag atccgcatca
    32161 tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag aaaaaactcg
    32221 gtcttcagga aaaataagac tgcaaaagcg tcaaaaggcc ggattttccg gcctttttta
    32281 ttactgggga tcgacaaccc ccataaggta caatcctcgc tttcttcacc catcagggac
    32341 aaaaaatgga cactcgtttt gttcaggccc ataaagaggc gcgctgggcg ctggggctga
    32401 cccttttgta tctggcagtt tggttagtag ccgcttactt acctggcgtt gcccccggtt
    32461 ttaccggctt tccgcgctgg tttgagatgg cctgcatcct gacgccgcta ctgtttattg
    32521 gactgtgctg ggcgatggtg aaatttatct atcgcgatat cccactggag gatgacgatg
    32581 cagcttgaag taattctacc gctggtcgcc tatctggtgg tggtgttcgg tatctcggtt
    32641 tatgcgatgc gtaaacggag caccggcacc ttccttaatg agtatttcct cggcagccgc
    32701 tctatgggcg gtattgtgct ggcgatgacg ctcaccgcga cctatatcag tgccagttcg
    32761 tttatcggcg ggccaggagc tgcttataaa tacgggctgg gctgggtatt gctggcgatg
    32821 attcagcttc ctgcagtctg gctttcactc ggtattctcg gcaagaagtt tgcgattctt
    32881 gcgcgccgct acaatgcagt gacgctgaac gatatgctgt ttgcccgcta ccagagtcgt
    32941 cttctggtgt ggctggcgag tttgagtttg ctggttgcgt tcgttggtgc gatgaccgtg
    33001 cagtttatcg gcggtgcgcg cctgctggaa accgcggcgg gtattcctta tgaaaccggg
    33061 ctgctgattt ttggtatcag cattgcgtta tataccgcct ttggtggctt tcgcgccagc
    33121 gtgctgaacg acaccatgca agggcttgtg atgctgattg gcaccgttgt gctgcttatt
    33181 ggcgtggtac atgccgctgg cggcttaagc aacgcagtac agaccttgca aaccatcgat
    33241 ccgcaactgg ttacgccaca aggcgctgac gatattctgt cgcctgcctt tatgacgtcg
    33301 ttctgggtac tggtgtgctt tggcgtgatt ggcctgccgc atactgcggt gcgctgtatc
    33361 tcttataaag acagcaaagc agtacaccgg gggatcatca tcggtacgat tgtagtcgca
    33421 atcctgatgt tcggtatgca cctggccgga gcgttaggtc gtgcagtgat ccccgatctc
    33481 accgtaccgg atctggtgat cccgacgtta atggtaaaag tgctgccacc gtttgctgcc
    33541 ggaatcttcc tggctgcacc tatggctgcg atcatgtcga cgatcaatgc ccagttgctg
    33601 caaagttccg ctacgatcat taaagatctc tatctgaata tccgtccgga tcaaatgcaa
    33661 aacgagacgc gtctgaagcg gatgtcggcg gtaattacgt tagttctcgg cgcgttgctg
    33721 ctgcttgccg cctggaagcc gccagaaatg atcatctggc tgaatttgct ggccttcggt
    33781 gggctggaag ccgttttcct gtggccgctg gtgctgggtc tttactggga acgcgccaac
    33841 gccaaaggcg cgctaagtgc gatgatcgtt ggcggcgtgc tgtatgccgt actcgcgacg
    33901 ctgaatattc agtacctggg cttccaccct attgtgccct cgttactact aagtttgctg
    33961 gctttcctgg tcggaaaccg tttcggtaca tccgtcccgc aagctaccgt tttgactact
    34021 gataaataaa gagttttgcc atgccttgga tccaactgaa actgaacacc accggcgcga
    34081 acgcggaaga tcttagcgat gcgctgatgg aagcgggtgc cgtttctatc acttttcagg
    34141 atacccacga tacgccagta tttgagccgc tgccgggcga aacgcgcctg tggggcgaca
    34201 ccgatgtgat tggtctgttc gacgctgaaa ccgatatgaa cgacgtggtg gcgattctgg
    34261 aaaaccatcc gctgctcggc gcaggcttcg cgcataaaat cgaacaacta gaagataaag
    34321 actgggagcg cgaatggatg gataatttcc acccgatgcg ctttggtgaa cgactgtgga
    34381 tctgccctag ctggcgtgat gtgccggacg aaaacgccgt caacgtgatg ttagatccag
    34441 ggctggcgtt tggtacgggt acccatccaa ccacctctct gtgcctgcaa tggctcgaca
    34501 gcctcgattt aaccggtaaa acagtcatcg actttggctg tggttccggc attctggcga
    34561 tcgcggcgct gaaactgggt gcagcaaaag ccattggtat tgatatcgat ccgcaggcga
    34621 ttcaggccag ccgcgacaac gccgaacgta atggcgtttc tgaccgtctg gaactctact
    34681 taccgaaaga tcagccagaa gaaatgaaag ccgacgtggt ggtcgctaac atccttgcag
    34741 gcccattacg tgaactggca ccgttaatca gcgtcctgcc ggtttcaggc ggtttgctgg
    34801 gcctttccgg tattctggca agccaggcag agagcgtttg tgaagcttat gccgatagct
    34861 tcgcactgga cccggtcgtg gaaaaagaag agtggtgccg tattaccggt cgtaagaatt
    34921 aaccttcgca tcgccgtagg gtgacgcggg gcgaagtgcg agcaagctca caaaaggcac
    34981 gtaaatttgc cggttattta cgcaaatttg cgtgccaaaa ttttcattca taaagaaaaa
    35041 ttgagaactt actcaaattt ctttgagtgt aaattttagt cactattttc taatatgatg
    35101 atttttatga gtaattatcg caccacgctc attttaaatg caattctttg atccatctca
    35161 gaggattggt caaagtttgg cctttcatct cgtgcaaaaa atgcgtaata tacgccgcct
    35221 tgcagtcaca gtatggtcat ttcttaactc atgcgcatcg gacaatatca gctcagaaat
    35281 cgcctgatcg cagcgcccat ggctggcatt acagacagac cttttcggac gttgtgctac
    35341 gagatgggag ccggattgac agtatccgag atgatgtctt ctaacccaca ggtttgggaa
    35401 agcgacaaat ctcgtttacg gatggtgcac attgatgaac ccggtattcg caccgtgcaa
    35461 attgctggta gcgatccgaa agaaatggca gatgcagcac gtattaacgt ggaaagcggt
    35521 gcccagatta ttgatatcaa tatgggttgc ccggctaaaa aagtgaatcg caagctcgca
    35581 ggttcagccc tcttgcagta cccggatgtc gttaaatcga tccttaccga ggtcgtcaat
    35641 gcagtggacg ttcctgttac cctgaagatt cgcaccggct gggcaccgga acaccgtaac
    35701 tgcgaagaga ttgcccaact ggctgaagac tgtggcattc aggctctgac cattcatggc
    35761 cgtacacgcg cctgtttgtt caatggagaa gctgagtacg acagtattcg ggcagttaag
    35821 cagaaagttt ccattccggt tatcgcgaat ggcgacatta ctgacccgct taaagccaga
    35881 gctgtgctcg actatacagg ggcggatgcc ctgatgatag gccgcgcagc tcagggaaga
    35941 ccctggatct ttcgggaaat ccagcattat ctggacactg gggagttgct gcccccgctg
    36001 cctttggcag aggttaagcg cttgctttgc gcgcacgttc gggaactgca tgacttttat
    36061 ggtccggcaa aagggtaccg aattgcacgt aaacacgttt cctggtatct ccaggaacac
    36121 gctccaaatg accagtttcg gcgcacattc aacgccattg aggatgccag cgaacagctg
    36181 gaggcgttgg aggcatactt cgaaaatttt gcgtaaacag aaataaagag ctgacagaac
    36241 tatgttcgaa caacgcgtaa attctgacgt actgaccgtt tctaccgtta actctcagga
    36301 tcaggtaacc caaaaacccc tgcgtgactc ggttaaacag gcactgaaga actattttgc
    36361 tcaactgaat ggtcaggatg tgaatgacct ctatgagctg gtactggctg aagtagaaca
    36421 gcccctgttg gacatggtga tgcaatacac ccgtggtaac cagacccgtg ctgcgctgat
    36481 gatgggcatc aaccgtggta cgctgcgtaa aaaattgaaa aaatacggca tgaactaatt
    36541 caggttagct aaatgcttga ttaaaaaggc gctactcggc atggggaagc gcctttttta
    36601 taggtgtcac aaagggagtg accatgagaa caggatgtga accgacccgg tttggtaatg
    36661 aagctaagac cattattcac ggtgatgcgc ttgccgaact taaaaagatc cccgccgaaa
    36721 gtgtcgatct gatctttgcc gacccaccat ataacatcgg taaaaatttt gatggtctga
    36781 tcgaagcctg gaaagaagat ctgtttatcg actggctgtt tgaagtgatt gcagagtgcc
    36841 accgcgttct gaaaaagcag ggcagcatgt acatcatgaa cagcacggaa aacatgccct
    36901 ttatcgatct ccagtgccgc aagcttttta ccatcaaaag tcgcatcgtc tggtcatatg
    36961 acagttctgg agtacaggcg aaaaaacact acggctccat gtacgaaccc atcctgatga
    37021 tggtgaaaga cgcaaagaac tacacattca acggtgatgc tattctggta gaagccaaaa
    37081 ccggatcgca gcgcgcgttg atcgattatc gcaaaaatcc tccacagcca tacaatcatc
    37141 aaaaagtacc gggtaacgtc tgggattttc cgcgcgtgcg ttatttgatg gatgaatatg
    37201 agaaccaccc gacgcaaaaa ccggaagcct tactgaaacg cattattctc gcctcttcca
    37261 acccaggcga tatcgttctc gacccgtttg ccggtagctt tactaccggt gccgtagccg
    37321 tctccagcgg gcgaaaattc attggtattg agatcaacag cgagtacatc aaaatggggc
    37381 ttcgacggct ggatgtcgcg tcgcattact ctgcggaaga actggcgaaa gtgaaaaaaa
    37441 gaaagaccgg caacctgtca aaacgaagcc ggttaagcga agttgacccc gatctcattg
    37501 caaagtaaag gaagtgtaag cctggttttc agattattca tttcgtgtat atttcctgcc
    37561 agacttggtt aaacatacac aggctcaggt aatgattcgc aagtattggt ggctcgtcgt
    37621 tttcgctgtc ttcgtttttc tgtttgatac tttactgatg cagtggattg aactgctggc
    37681 aacagaaaca gacaaatgcc gcaatatgaa ctcagttaat ccactaaaac tggttaactg
    37741 tgacgaactg aattttcagg acagaatatg aatttacatg acacttaatt cattcgtttg
    37801 atgaattaat ttcgttatgt tttcatctgg catgaacatt cttaatacgt tatcgaccag
    37861 agcgggggct tgtttataaa gatcataacc cgccatattc attaaccagt tttgaacaat
    37921 tccgctgaag gcaccattaa taataatcat cacaacatct aaatcgaggt tatttgctac
    37981 acaaccttgt tgctgacacg cctgcaatac ttcgcggaga gtctgcggat tgaagcccat
    38041 cttttcgcgt atcactccct cggccagcat ctcatcatta aattcacatt tgtgatataa
    38101 gattttcagc aacgcctgct ggcggggaat tttggcaata tattgcaagc cgacaatcaa
    38161 tttttcacgc aattgttgaa acgggtcatg ctctaatcca gccgtcaagt gttcctggat
    38221 taactcccgc aatgaaggct gttgcaacca catctcatta aacagttgag tcttgttttc
    38281 gaagtgccag tagatagcgc cacgcgtaac gttagcagcg tcggcaatgt cgttgagtgt
    38341 cgtcttgctt acgccatgct gcgcaaactg ggcgatggca gtttcaatca gttcttgccg
    38401 ggtcttcaga gcttcggctt tggttctttt tgccatgatt aattattcag gaaataaata
    38461 tattcgacac agagtgagaa aatagcgaag gttaatctat cacctaatgt gtattta
//
LOCUS       DAZGCS010000041        38391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGCS010000041 DAZGCS010000000
VERSION     DAZGCS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.041"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            113..2356
                     /gene="pdeF"
                     /locus_tag="ACHK01_002153"
     CDS             113..2356
                     /gene="pdeF"
                     /locus_tag="ACHK01_002153"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO8097104.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2395..3936)
                     /gene="ppx"
                     /locus_tag="ACHK01_002154"
     CDS             complement(2395..3936)
                     /gene="ppx"
                     /locus_tag="ACHK01_002154"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO8097105.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3941..6007)
                     /gene="ppk1"
                     /locus_tag="ACHK01_002155"
     CDS             complement(3941..6007)
                     /gene="ppk1"
                     /locus_tag="ACHK01_002155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO8097106.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6178..6816)
                     /gene="purN"
                     /locus_tag="ACHK01_002156"
     CDS             complement(6178..6816)
                     /gene="purN"
                     /locus_tag="ACHK01_002156"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO8097107.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6816..7853)
                     /gene="purM"
                     /locus_tag="ACHK01_002157"
     CDS             complement(6816..7853)
                     /gene="purM"
                     /locus_tag="ACHK01_002157"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO8097108.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8178..8804
                     /gene="upp"
                     /locus_tag="ACHK01_002158"
     CDS             8178..8804
                     /gene="upp"
                     /locus_tag="ACHK01_002158"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO8097109.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8890..10179
                     /gene="uraA"
                     /locus_tag="ACHK01_002159"
     CDS             8890..10179
                     /gene="uraA"
                     /locus_tag="ACHK01_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO8097110.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10274..10975
                     /gene="hda"
                     /locus_tag="ACHK01_002160"
     CDS             10274..10975
                     /gene="hda"
                     /locus_tag="ACHK01_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO8097111.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11113..11472)
                     /gene="arsC"
                     /locus_tag="ACHK01_002161"
     CDS             complement(11113..11472)
                     /gene="arsC"
                     /locus_tag="ACHK01_002161"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO8097112.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11493..12956)
                     /gene="bepA"
                     /locus_tag="ACHK01_002162"
     CDS             complement(11493..12956)
                     /gene="bepA"
                     /locus_tag="ACHK01_002162"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO8097113.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13169..14230
                     /gene="yfgO"
                     /locus_tag="ACHK01_002163"
     CDS             13169..14230
                     /gene="yfgO"
                     /locus_tag="ACHK01_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8097114.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14268..15119)
                     /gene="focB"
                     /locus_tag="ACHK01_002164"
     CDS             complement(14268..15119)
                     /gene="focB"
                     /locus_tag="ACHK01_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO8097115.1"
                     /translation="MHEKQTLFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGV
                     FMAIGFTFYLSVIADTPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSR
                     GVISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHT
                     FTESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIP
                     FAIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRA
                     IYLRQES"
     gene            complement(15137..17149)
                     /gene="hyfR"
                     /locus_tag="ACHK01_002165"
     CDS             complement(15137..17149)
                     /gene="hyfR"
                     /locus_tag="ACHK01_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO8097116.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHFDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRSMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17179..17592)
                     /gene="hyfJ"
                     /locus_tag="ACHK01_002166"
     CDS             complement(17179..17592)
                     /gene="hyfJ"
                     /locus_tag="ACHK01_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO8097117.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17585..18342)
                     /gene="hyfI"
                     /locus_tag="ACHK01_002167"
                     /pseudo
     CDS             complement(17585..18342)
                     /gene="hyfI"
                     /locus_tag="ACHK01_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18339..18877)
                     /gene="hyfH"
                     /locus_tag="ACHK01_002168"
                     /pseudo
     CDS             complement(18339..18877)
                     /gene="hyfH"
                     /locus_tag="ACHK01_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
     gene            complement(18887..20554)
                     /gene="hyfG"
                     /locus_tag="ACHK01_002169"
     CDS             complement(18887..20554)
                     /gene="hyfG"
                     /locus_tag="ACHK01_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO8097118.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20544..22130)
                     /gene="hyfF"
                     /locus_tag="ACHK01_002170"
     CDS             complement(20544..22130)
                     /gene="hyfF"
                     /locus_tag="ACHK01_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO8097119.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(22135..22785)
                     /gene="hyfE"
                     /locus_tag="ACHK01_002171"
     CDS             complement(22135..22785)
                     /gene="hyfE"
                     /locus_tag="ACHK01_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO8097120.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22797..24027)
                     /locus_tag="ACHK01_002172"
                     /pseudo
     CDS             complement(22797..24027)
                     /locus_tag="ACHK01_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24044..24991)
                     /gene="hyfC"
                     /locus_tag="ACHK01_002173"
     CDS             complement(24044..24991)
                     /gene="hyfC"
                     /locus_tag="ACHK01_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO8097121.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25002..27020)
                     /gene="hyfB"
                     /locus_tag="ACHK01_002174"
     CDS             complement(25002..27020)
                     /gene="hyfB"
                     /locus_tag="ACHK01_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO8097122.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQSLRVVFSALFRLRQQFDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27020..27637)
                     /gene="hyfA"
                     /locus_tag="ACHK01_002175"
     CDS             complement(27020..27637)
                     /gene="hyfA"
                     /locus_tag="ACHK01_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO8097123.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(27890..28360)
                     /gene="bcp"
                     /locus_tag="ACHK01_002176"
     CDS             complement(27890..28360)
                     /gene="bcp"
                     /locus_tag="ACHK01_002176"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO8097124.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28360..28932)
                     /gene="gcvR"
                     /locus_tag="ACHK01_002177"
     CDS             complement(28360..28932)
                     /gene="gcvR"
                     /locus_tag="ACHK01_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO8097125.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29078..29956
                     /gene="dapA"
                     /locus_tag="ACHK01_002178"
     CDS             29078..29956
                     /gene="dapA"
                     /locus_tag="ACHK01_002178"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO8097126.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            29973..31007
                     /gene="bamC"
                     /locus_tag="ACHK01_002179"
     CDS             29973..31007
                     /gene="bamC"
                     /locus_tag="ACHK01_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO8097127.1"
                     /translation="MAYSVQKSRLAKIAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31220..31933
                     /gene="purC"
                     /locus_tag="ACHK01_002180"
     CDS             31220..31933
                     /gene="purC"
                     /locus_tag="ACHK01_002180"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO8097128.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32088..32315
                     /gene="vapB"
                     /locus_tag="ACHK01_002181"
     CDS             32088..32315
                     /gene="vapB"
                     /locus_tag="ACHK01_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO8097129.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32315..32713
                     /locus_tag="ACHK01_002182"
     CDS             32315..32713
                     /locus_tag="ACHK01_002182"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO8097130.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32860..33723
                     /gene="ypfJ"
                     /locus_tag="ACHK01_002183"
     CDS             32860..33723
                     /gene="ypfJ"
                     /locus_tag="ACHK01_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO8097131.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33738..35753
                     /gene="tmcA"
                     /locus_tag="ACHK01_002184"
     CDS             33738..35753
                     /gene="tmcA"
                     /locus_tag="ACHK01_002184"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO8097132.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35827..36525
                     /gene="ypfH"
                     /locus_tag="ACHK01_002185"
     CDS             35827..36525
                     /gene="ypfH"
                     /locus_tag="ACHK01_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8097133.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36635..36835)
                     /gene="ypfN"
                     /locus_tag="ACHK01_002186"
     CDS             complement(36635..36835)
                     /gene="ypfN"
                     /locus_tag="ACHK01_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO8097134.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36863..37990)
                     /gene="dapE"
                     /locus_tag="ACHK01_002187"
     CDS             complement(36863..37990)
                     /gene="dapE"
                     /locus_tag="ACHK01_002187"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO8097135.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37994..38350)
                     /gene="yffB"
                     /locus_tag="ACHK01_002188"
     CDS             complement(37994..38350)
                     /gene="yffB"
                     /locus_tag="ACHK01_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO8097136.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9329 a         9895 c        10103 g         9064 t
ORIGIN      
        1 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
       61 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
      121 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
      181 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
      241 ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt tcagctgggc
      301 ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
      361 cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt ggggcggtta
      421 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
      481 gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
      541 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
      601 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
      661 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
      721 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
      781 aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
      841 caattatacc ttgtcattat tgttgcccct gatgatgtgg ggagcaatgc gctatggtta
      901 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
      961 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
     1021 ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc gagcggtagt
     1081 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
     1141 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
     1201 catggaaatg ctggttaaga actatggcat catgctgcgg attcaataca agcaaaaact
     1261 ttctcactgg ctgtcaccct tgctggaacc gggtgaagat gtttatcagc tttcgggtaa
     1321 cgatctcgcg ctgcggctga atacagaatc gcaccaggag cgcattaccg cactggatag
     1381 ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc agattggcgt
     1441 cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg gagagctaaa
     1501 tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc
     1561 aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac tacagcaggc
     1621 gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc gtggtgatgt
     1681 ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatcg gccccgatag
     1741 cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt gggtcattga
     1801 gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc gttttgctat
     1861 taatctgtct ccaacctcgg tatgtcaggc tcgttttcct gttgaagtca gtcagttgct
     1921 ggctaaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct
     1981 gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag gctgccagat
     2041 tgcgattgat gatttcggca ccggctacgc cagctatgca cggcttaaaa atgtgaatgc
     2101 cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata gtctggatta
     2161 tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg tagtggcaga
     2221 gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg attatatgca
     2281 gggttatctt attggtaagc cgcagccgtt aattgatacg ttgaatgaaa tcgaacccat
     2341 tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa agcattaagc
     2401 ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca cgccttccca
     2461 gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa accagtcatg
     2521 cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt
     2581 ggttgcctga cgttgattgt ttagtaatac gccaaggcgc aatagctgta tcagtggcag
     2641 gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg
     2701 gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc
     2761 actgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga tattcaaccc
     2821 gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat gcgccagctt
     2881 cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca ctcgtcgggc
     2941 ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac tacgcacatc
     3001 ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga
     3061 aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg ccagtccagg
     3121 aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg cgaaattacg
     3181 gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta tcccgtcttt
     3241 ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag cgcccattgc
     3301 cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt gtgctgccgc
     3361 catacgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaagt aaagctgggc
     3421 aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat tttcaccaat
     3481 caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt tctccggttg
     3541 ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa taatttcaat
     3601 cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg
     3661 cagcgtatgg gtgccgacta tacagacgct ggcaggagaa aacccttgta gccgttcggc
     3721 aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca tattatctgg
     3781 ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct gcatggcacc
     3841 atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa
     3901 ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt tgttcgagtg
     3961 atttgatgta gtcgtaaatc gccaactgcg cccgtacttt gcggcgattg ccgcggggaa
     4021 cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata
     4081 tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggt gtcgccactt
     4141 caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag acctttttat
     4201 cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca acaatactga
     4261 tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac attccgcgaa
     4321 ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca accaggcctt
     4381 tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc tgcgcgttgg
     4441 cgatttcgcg gtctaccatt tcatataaca gacggcgaga gttttgcggt gacaccatta
     4501 aataatcaaa tgtcaccggg cggtatgggt tttcaataaa gttaaatacc cgccgtactt
     4561 cgttggtgat acgcgcatct gcggtcagca acgaatagtc agtataaaga cgcgcggttt
     4621 tttcgttaaa gttcccggtc ccgatgtgtg cgtaacggac cacttcaccg ttttctttac
     4681 gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag ataacgtgca
     4741 cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtcg aaacgcgcct
     4801 gtaactcaac caccacggtg actttcttac cgttatgtgc ggcgtggatc atcgagtcga
     4861 tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt acgctcgggt
     4921 cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtgtgataa ggataataga
     4981 gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct ttatcaaacc
     5041 aaatgtggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg acattgggga
     5101 aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag tcgtagcggg
     5161 aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg cgctgataaa
     5221 caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatt aactccatca
     5281 ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc ttcattgaat
     5341 aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcgagg cagtaacgca
     5401 gaatgttatc caaaagaatc atcggcttgc gtcgacgcgg tgcttctggc ggtaaattca
     5461 caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg gtatcgccac
     5521 ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact aagtcagtgt
     5581 caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa taatgacgca
     5641 gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc tggttgcgcg
     5701 ccatctccag caatagctcg ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc
     5761 gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt tcttcgctaa
     5821 taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca aggttattgg
     5881 aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc gccgcttcct
     5941 gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat agcttttcct
     6001 gacccattac cacttttact ccgttttatt cacgggacac tgcctggata ttatggcgag
     6061 caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct ggagcttgcg
     6121 atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta cggggcatta
     6181 ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc
     6241 gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa tagcgtgttc
     6301 ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata ccgggacttt
     6361 cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc
     6421 gtgctcttca tcaccatttt ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg
     6481 cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa acgccgggct
     6541 gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt cgatttcatg
     6601 aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga gcgtatgcgt
     6661 tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac
     6721 tgcccgtacg gtgcctttaa ttttgttagt tttacaggcg tcaataattg cctgtaaatt
     6781 acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg
     6841 gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttgga gtttagcagg
     6901 gcgagggctt tgtccacttc cggagcaggc agggcgataa tcatcccgac gccgcagttg
     6961 aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttgaacact
     7021 tccggccact gccaggaaga ttcatcaatc actgcctgag tattatctgg caatacgcgt
     7081 ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt
     7141 tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag atgatcggct
     7201 aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc aagaattttg
     7261 cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg
     7321 ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa
     7381 cccgcgacat cgtaatcctc accgtgatac atccccggca tttctgccgt ttcgccaccc
     7441 accagtgaac agcccgattg cagacatcct tccgcgatac cgctgatcac cgctgaagcg
     7501 gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ctctgcacct
     7561 tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt
     7621 tttaagtcca tcgccagacg cagcttggta cctacgccgt cagtaccgga aaccagcaca
     7681 ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgccaag accgcccatc
     7741 acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc
     7801 gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg
     7861 gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc
     7921 aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttgccca ggcacgcggc
     7981 gaaagaagac ttgtgccagg gtaaaggtta gttttcggat ggaataatct tctttcataa
     8041 ccatttgaat ataaaataac tttatctcaa accgttatca ttttgactaa agtcaacgaa
     8101 aagaatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt gtggctgccc
     8161 ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc
     8221 tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc gcttccgaag
     8281 tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg
     8341 aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt accgttgtgc
     8401 caattctgcg tgcgggtctt ggtatgatgg acggtgtgct ggaaaacgtt ccgagcgcgc
     8461 gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta ccgtacttcc
     8521 agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca atgctggcaa
     8581 ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc agcagcatca
     8641 aagttctggt gctggtcgct gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg
     8701 acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga tacattattc
     8761 cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata aaaataatta
     8821 aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag
     8881 gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca
     8941 atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc
     9001 ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat
     9061 ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca
     9121 ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc
     9181 ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac
     9241 gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg
     9301 ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa
     9361 accatcatca tctctattac caccctggcg gtcacggttt taggttccgt gctgtttcgt
     9421 ggtttcctgg caattatccc gattttaatt ggcgtgctgg tgggctacgc gctctctttc
     9481 gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc
     9541 ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg
     9601 gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg
     9661 ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc
     9721 ggcttctttg gctctacgcc aaataccact tacggagaaa acattggcgt gatggcgatc
     9781 acccgtgttt acagtacctg ggttatcggt ggggcggcga ttttcgctat cctgctttcc
     9841 tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt
     9901 tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa
     9961 gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc
    10021 agtggcgcga aggtaaacat cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc
    10081 gtcggtatcg gcttaagttt gattttcaaa ctgatcagtg tgctgcgccc tgaagaagtg
    10141 gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg
    10201 ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct
    10261 ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga
    10321 cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg cgctgcaaaa
    10381 cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg
    10441 cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta
    10501 tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt
    10561 gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc
    10621 gattttcgat ctctacaatc gaattctgga atcgggcaaa acacggttgt tgatcaccgg
    10681 cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg
    10741 ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt
    10801 acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg
    10861 gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat
    10921 taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag
    10981 gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag
    11041 tattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg
    11101 atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc
    11161 tttgccattc gcgaccacaa tcgggcgctc cattagcttc ggattatcca ccattgcctg
    11221 aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc
    11281 tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag
    11341 cgttgccgca tcggcgggtg tctcaaggta gagtaccact tctggctcca cgccgttttc
    11401 tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac
    11461 ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt
    11521 tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct
    11581 aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg
    11641 agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag cgcggcttcc
    11701 gcctgtgcca gtaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgattc
    11761 agaatattgg ccgcttcttg tggttgaccg ccttgcagat aagcgttcgc caggttgagc
    11821 tgcaacaccg gattggtgcg taaatcgcgg gcatttttca gacgattgat cgcctcattg
    11881 gctttgtttt gcccaagatc gatatcagta gccagatcga gataccatgc gttgccaggt
    11941 tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc
    12001 gcctgtaaag cacgaccata ttgtgctgct cgttgctgac gaacgtttcc tttcgcccat
    12061 tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc
    12121 gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcgggcgcat ctgattagca
    12181 cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcaataa aatttccggc
    12241 ggacgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg catcgcctgc
    12301 ggatcgaatc ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt
    12361 tgctgggtga aactgatcat cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc
    12421 atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc
    12481 agcggcgcgc tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg
    12541 gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg
    12601 gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg
    12661 atcagaaaga aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc
    12721 agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc
    12781 tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg
    12841 gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca
    12901 atggtcatag cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc
    12961 tctatcctgt atttctgaag atagtcattt gaccgatact gttaaatatc gttcccaccg
    13021 cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga
    13081 atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc
    13141 ccgctcttga ggttcaggga aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg
    13201 ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat
    13261 tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt
    13321 gctggaatgg ccaactgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc
    13381 gattgtattg gtggtttttg tcggtatatt gctactgatg gcgttcgtgg tactgcctat
    13441 cgcctggcaa cagggcatct acttaattcg cgatatgccg gggatgctca ataagctttc
    13501 tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggacgcgg gcattattga
    13561 tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat
    13621 ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc tggtgccatt
    13681 gatggtcttc ttcctgctga aagacaaaga gcagatgctg aacgccgttc gtcgggtgct
    13741 gccgcgcaac cgtggactgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa
    13801 ctatatccgc ggcaaagtgc tggagatgat cgtggtgggg atcgccacct ggctggggtt
    13861 cttgctcttt gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggttct
    13921 tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca
    13981 gtttggcgca ggcacggaat tctggagctg cttcgcggtg tatctgatta ttcaggcgct
    14041 ggacggcaac ctgctagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt
    14101 gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc
    14161 cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc
    14221 gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga
    14281 cgtaaataaa tagcccgata acacatactc accagcactg caccgccgat aatattaccc
    14341 agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc cggaaaattg
    14401 tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg aatggcaatg
    14461 gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agccactggc gacaaacagg
    14521 gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca ataactcatc
    14581 cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga ttcagtaaat
    14641 gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca
    14701 ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc tgccagatta
    14761 ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aactaataac gccccgactt
    14821 tttgccatta ccgtcattac cgacgaggtg aacaggttgg tgccgcaaac cgccagcaaa
    14881 ataaagccaa gtgtaaagca aaggccgccc accagatggg ttaatgcctg tgaagacggg
    14941 gtatcggcga taacggaaag gtaaaaagta aatccgatcg ccataaacac gccagcggac
    15001 atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc aatccgttca
    15061 gcgattgccg cttttctggc gctcaactgc aagtcgaaga gagtttgttt ctcatgcata
    15121 ctcctgcaaa agcagattac aacacctcgc gaaccgagat tcccagacgc tgcattcgtg
    15181 acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc ccggcaacaa
    15241 tgccattggt ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg
    15301 gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg
    15361 tcggcagtaa acggctttgt cggacattaa gatgtaaatt cagactgtta ccgcgagtca
    15421 gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac gttgcccggc cagtcccacg
    15481 acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc atatggcgcg
    15541 ccattttttg cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg
    15601 gcggcaattc cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat
    15661 cttcaaccat ttgccacaaa tcacggttgg tggcggcaat gactctgaca tttaccggga
    15721 tcgttctact cccgccgaga cgctcaatct cccgttcctg caatacgcgc agcagtttag
    15781 gctgaagttc taacggcaga tcgccaattt catcgagaaa caacgtgccg ccatcggcaa
    15841 tttcaaaacg accacgatgg gtattaatcg caccagtaaa cgcccctttg tcatgaccga
    15901 ataactcact ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct
    15961 tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat cacctctttg ccggttccgg
    16021 tttcaccgca aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagta
    16081 ggtcttccat tgcctggctt tgatagataa tgtcaccgat gcccagattc gaaaggagct
    16141 gctcgctaag ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctacgcc
    16201 aggcatcggc attgccaacg gcaatagcga tgcgatcggc tatgtgttgt agtagctggc
    16261 agttttcctc actaaagaga gtggaagagg tatgcgccag caacaatgca cccggtgtat
    16321 ggttgccaaa ggtaagcggt ataaggagcg cagattcgca gccgttagat gcaagtaaga
    16381 gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt
    16441 gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc aggcatactg cgtggcagac
    16501 acgcacaatg tgaggcagaa agatcgctgc accacaggct aaacttctcg ttctttcgat
    16561 gatcgcccag taccatactg actgaagcca gaccgaaaaa atgatggatc tcacgagcga
    16621 cgtcagcgat cagatcgtcg agatcaaaat gtgacagcac tgcattagta atatccacca
    16681 gtacccgatg atgatcgcga tctttctcca gcaaacgcag cgccacctgc tgttcttcat
    16741 cattgagcca tgattgcaga accggggtga ttatccgcgc cagccagtta aacagtgtgc
    16801 aatcctgggg agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc gccattccac
    16861 tactgccgag aaggacggcg tgacgcgaat aatcgaccat ctcgccctga tactctgttg
    16921 ttgccgtcac atcgctgcca tcatcatggt agcggtaata gaagttgctt ttgagaacat
    16981 gcagttcaat aagttgtacc gggctgaacg gcggcggcat cgggatgaag gcgcgtaata
    17041 aagacgcctt accgtgtttc tgtgctaacc gctccgcgag cattccgttt accgcttcaa
    17101 ttgttattcc ttgtggcggg gcaaacatcg cctcgtctga catagccatc aacagtcctt
    17161 attaatgaga aatccttctc acgcaatttt tctggccatc agatagatgg ccggttcctg
    17221 cacaatttca tccaacagat gaatgaattg aatgctacgc gcctgccaca cagacgtcgc
    17281 gtcatctgcc agtggcgcga atgcccgggt caacagtgcg gtgtggctgg cgtcaataac
    17341 aatctcacca aaagtcatca cccccagcat cttacgtcgg gcttgctcct cttcgaccag
    17401 tgcaagccaa tcttcgtatt ccgtcagtgg gcagcggaag gcgacattca gacaatcaat
    17461 cacgccaacg tgatgaccga tagccagcga gtaatacatt acctgggagc tgtgttccgg
    17521 catctcgtcg ctactggcga caaacttttt tcgcagcgtc cagaaaacaa tttccccgca
    17581 ctcctcagcc atgtaacccc cggagaacgc gaaagagttg ctgaacgata ctattgagac
    17641 gcggatcgtc agcttcgcgc aaccaggtgt taacccgatt tcctgtagga tcgtcgctta
    17701 aatggcgcag gagccgatcg caaatttctc gcccctgacg atagcccgcc agctgccgcg
    17761 cttctcgctc aatggcgata cgctgtgatg gcgggatctg cggccacagc ggttgcatgg
    17821 tcaccccggt gggatcgcga taatccacag cgtgaatctt ctgttgcagc aaaccgagcg
    17881 ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg gcagccgggg atccaaacat
    17941 caatggggac aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg aaaatccgcc
    18001 gccggcaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc
    18061 ccgaagtgca ggcatacgca tcgcccgggt gagtgcgcca gtaaataaca aaatatcggc
    18121 gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata ctggtgtaat
    18181 ggcggcaaaa atttcgattt cacaggcgtt gcagccaccg caatcgacgc gataaacgta
    18241 ggctgagcga cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag
    18301 cgtgatgggc tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc
    18361 agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc cgggcagacg
    18421 ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat
    18481 tcagcagcca gtgcgacggt tttttgctgg gcaaacgggc gttcgcaacg gctgcaacgt
    18541 tgtagatgga acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag
    18601 ttattggtaa gctgatggct ctggtcgggc acacttcttc acaacgtccg cagtaaatac
    18661 aacgcctcag atagagctgc caggtgcgcg aattttgctg gtcgtcggtc tggatagtca
    18721 gcgcatttgc cggacaagca caggcgccgc aggcaataca ttggctgggc atcaggtccg
    18781 gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa gggatatttc accgtcgcgg
    18841 ttccggcgcg cataatagtt ttcagtaact tcagcatctt ctgtccttac ttcagcggcg
    18901 aacggttacg atcaatgccg tagcgttcga tctctttata cggcacggtt tttgactggc
    18961 gcttgcgcac atctaccagc gtcacacggt cggtacagga gtagcaggga tcaaggctac
    19021 cgataatcag cggtgcgtca gaaacggtat tgccgcgcag catgtaacgc aacaccggcc
    19081 agttggcgta ggtggcggca cggcagcgcc agcggaacaa tttttggtta tcaccgagca
    19141 tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa gcccagcgcg aatgcgtgag
    19201 gtttatagct aaagccttcg gtcagcagtg gggtatccgg catgttgtcg agagcaaatt
    19261 ctagcattgc cagcgaatca aacgtctctt tgacacggac catcacgcgg gagaaaacat
    19321 cgccgccggt aaaggtaaac agtgtttttg gaatattgcc gtagtcggcg taggggtgat
    19381 caaagcgcaa atcacgggcg atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt
    19441 ccatattcgg cgtagcaagc agcatctcta ccagctccga cacgtcggcg cgcatctcgc
    19501 gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga acaccaccaa
    19561 tcagattcag accgtaggtt ttacgcgacc cgatcagcaa ttccgccatc gtcatcgact
    19621 tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa accaacgaaa tggcaggaga
    19681 ggccaaggtt aagcaaatga ctgtgtagcc gttcgacttc cagcagaatc gagcgaatag
    19741 tatgtgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg gtataggcca
    19801 cactgtgggc aaaaccgcaa atcccacaca cgcggtccga taagaaggtc acttcgttgt
    19861 agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac aggcggtaat
    19921 cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccagt tcatcggagg
    19981 tgatatgcag cgggccgaca gggatcacct gcgcatcgct gttgccctca ttgataaacg
    20041 gatacgtttc ggaatcagtc gtcggttcag ggcgcagtcg ataatccatc gcatctttgc
    20101 gcagcggatg catatcttcc ggccagtcat cgggcaacac caggcgacgc tgatccggca
    20161 ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag accgcggcag
    20221 ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt gccttcacca
    20281 cgatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg taatgaccgt
    20341 taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt tgataataaa
    20401 gataatgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc tgttcaggcg
    20461 tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg
    20521 cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac agatgcggtg
    20581 cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag
    20641 aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat atgcgttccc
    20701 atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca gccgagatca
    20761 ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgctgccat ccgtaccagg
    20821 cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag gtgattacgt
    20881 gccagtccgg cggtaatggt cataaattcg ctaagaaaaa tgttgaaggg cggcatccct
    20941 gccagcgcca gcgccagcgc accgccgcca aacagcacgg cggtaaatgg catgattttg
    21001 agcatcccac agacgacgtt gagatcgcgc gtgccgtact tgagcagtac attgccggaa
    21061 ccgcagaaca gcagcgtttt tgccagactg tggtttaagg tgtgcagcag cgcggcaaaa
    21121 attcccagtg gcccgccaat gcctagcgcc accgcgacca gtcccatgtt ctccacgctg
    21181 gagtacgcca gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc cgccacggca
    21241 accgacaaca tgccgaagat gagcaacaac cggttgggga aatcgctgcc gatggcctgg
    21301 cagataatga tgtaatagcg aattagcacc aacagcgcgc agttcagcaa tacggcggag
    21361 agcagggcgc tgaccggact cggtgcttca ctgtgagcat ccggcagcca ggcgtgcatg
    21421 ggaaatagcc cggttttggt gccaaagcca attagcacaa acacaaaggc caacagcatt
    21481 aatgttgggt caagcaagga cgattgctta agaacctcgc tccagaatat cgccatttct
    21541 gcctgcggca taacgctggc gacgttggcg tataccagca cggtaccgaa cagaccaaaa
    21601 gcgacaccaa cagtacaaat aatgatgtac ttccatgctg cttccagcga tgaacgctga
    21661 ccgtaaatgc ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc cgcccacatc
    21721 acaatcaggt tattgctggt aacaaccagc agcatggtaa acaaaaacag atggaagaaa
    21781 ccgtagtaat cgcacagcgt aacgggggaa agctcgccgt gtgccacttc atgacgcatg
    21841 tagccaatcg agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa
    21901 ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg
    21961 gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat
    22021 acagtcaccg tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa
    22081 aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc
    22141 cttcagcgcg gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc
    22201 cagtaacacc atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag
    22261 ttccggtgct cgccaggcaa gaagcgccag caccagatgg gagccgtttt ccatcaggca
    22321 gtaaccaaaa atttgccgca ggatattgcg ctggctgaca atgcacagca ggccaagcag
    22381 aaaatgacct aacgctaccg ccagcgccgg tttcagcccg gtagccatcg gtagcttcac
    22441 gggctgaacg acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg ccatcattgc
    22501 cggaccgaat aacgcttttt ccgggatgtt ctggggaata tttcgtgcag cgtaagtcat
    22561 gattaacggt accagcagca ctttggtgat aaaggcgctg gcggaccaga tcagcagttg
    22621 ctctgcggcg aacaggcacg agagagtggc gaaaatagac accagcacca gcgactggca
    22681 ggcgtaaaat ccgcatgaca ggcgatagct tttgacgcta atcacaaaca gcgatgtcag
    22741 catcatcagt cccgccagat tatttacgat catagaaccg gtcattcatc atctccttac
    22801 tgcaaccagg tcgcggcgat tacgctggaa atcagcaaca tcacaatcag tacaatcaac
    22861 accaggcgca ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct cggtttgcca
    22921 ggcacaacgc gaccaaacca gcgaataaac caggcgaaac tggcgaccga ttcaatcatc
    22981 agcagaatca tggcgggcag caggatccag tactccactg acaacgcaaa accggcagca
    23041 aacagcggga atttactgaa gaagccgttg aacggcggca cgccggtaat cgccagcgct
    23101 gccacgcaga aacccacgcc tggcaacggc agggtgtgca atacgccacg cagacgcggc
    23161 aacaagcgcg tgccgcagct gtaactcagc gcacctgcta caaggaaaaa caggctttta
    23221 gcgaacgcgt ggttgacgat gtaggcgatg ctgccctcca gtgccagccg cccactttca
    23281 ccatcgatgc ggcgtggaga taggcgctga tcggtgttgg cgcttccatt gcatccggta
    23341 gccacgcttg catcggtagc tgggccgatt tcccccacgc ggcaaacagg atgccgccat
    23401 aaaccagata acgtgcgtcg ccatgtaact cgctcatcgc gctaagcaca aacgttccgg
    23461 tctgcaaaaa cagcgtggcg gcggcaagat acaaccccaa cgaaccgata tgggtgataa
    23521 gcaacgcttt tagtgctgaa cgctgcgctt tatcgctctg gtaataactg atcaacgccc
    23581 aggagcagcc gcccgtaatt tcaaaaaaca acaactgacc gagcagcgtc gaggagagta
    23641 ccagtcccgc catcgcgccg ataaacacca gcaaaaatgc gtaataacga ttcgtgccgt
    23701 tatgcgggtg ttcgcgattt ttatccgtca gataacccgt ggagtagatc gtgaccagca
    23761 aaccgagaaa caccaccaca aacagaatca gcgtactcac gcgatcaatg accaggccaa
    23821 acaatgccac atcccccacg ttgaccaacg tcaacgtgac ggcaacttta tcggcctgat
    23881 aaaacgcgga gatcagcgac aacatgcaca gcgtggtcag tgcggcgaac aaaaccccca
    23941 ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa aaggcagcat
    24001 taacgtcgtc agagcaagat tttccatatt ccgtcagtgc tccttacaga ccggttaacc
    24061 agaagaccca tgcaagcgca gcaaggctga agccaagcca ggtcacatgg tgaatgagta
    24121 aaaaacgccc gcgtgccagc gtgttttctg cgattgaggc cagtacaaaa atcagcaaaa
    24181 ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc gagagaaagt tcttgcgcgc
    24241 gcccaaaggg cagaaacagg gccacaaaca gtgctgccat cacgacctgt ttcagcccca
    24301 gcccccattt cgctagcgcc agcccggcac cggaatattc ggtcagcggg ccttcctgta
    24361 attcctgttc tgcttcagca acatcaaagg gaatttttcc catctcaatg aagcaggcaa
    24421 aaccacaggc cagtaacgcc agtacggtgg ttagcggcga gttccagccc gttgccagcg
    24481 tattgctgat catctcgata tgcgtggaac ctgctatcag cgccaatacc agcagtgaga
    24541 gaatgagcat gggttcgacc agaatgccga gcgtcaactc gcgactggca ccgactcccg
    24601 caaacgggct tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac agggcaagaa
    24661 gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta ataaaaagtg
    24721 gtagggccat cgccagcacc agcatgctgc tgattaatac ccacggcatc aggcggaaca
    24781 tcagacctga agatataggc gcaacttcct ggcgtttaaa cagtttgtgg atatcgcgat
    24841 aatcctgcca gatccccggc ccgcggcggg agtgcatacg cgcgcgtatc tgccgggaaa
    24901 tacccgtaaa aagcggggtt agcatcagca gaatcacggc ctgtgctaac gcaaaaatga
    24961 ccagatatcc gtcgtaaaga gtttgtctca tggtgatttc cttagacggc aatagcgatt
    25021 agcagcacaa ccagtgcggc gaccacatac aggcaataga gacgaaagtc gccgctttgc
    25081 agatgctgta tttctttggc cagccgttgg gtggcgctca cgatggggcg gatcacccgc
    25141 tcatcccaga agggttctgt gctctgagcc ctggcggtga cgtgcgcaag acctttattt
    25201 agcctcagcg tagggtcgaa ctgttgtcgt agacgaaata gcgcagaaaa gaccacacgc
    25261 agcgactgca tcacgccatt gcctgacggg gccatcgcat tttcccagcc gtagccgcat
    25321 gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca cagccagtaa
    25381 aggccaggca gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg ggtgacctgt
    25441 gtatgaaacg tgccgggtac aagtgctatt ccgctggcga cagtgacggg aggggtattg
    25501 gtaaacgcat gggcaatatg cattatcttc ggtgccagcc aactggcact aagcgcaatt
    25561 aatacgcaga gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg cacttcctgt
    25621 gcctcttcag cgtgtgtact gcgcggcgca ccacagaaag taataccgta catttttacg
    25681 aagcacatta ctgccagccc accagtgact gccagcatta ccatagcaat aggacccgca
    25741 agttgtagcg ctacggcttc cacacgactt agtgagaaca gcgactgcca ggtgtaccat
    25801 tcgctgataa aaccattcag cggaggaatg gctgatatcg cgaggcaacc aatcaggcat
    25861 gctgcggctg tccacggcat ccgtttcgcc agtgccccca ttttttccat gtcgtgggta
    25921 tgcaaacgcg aaataatcgc gcccgcgccg agaaatagca gccctttgaa cagcgcatgg
    25981 ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg gtcgtgcagt
    26041 gacagaccga ccatcgccac accgactgcc agcaaaataa tgccgacgtt ttcgacggta
    26101 ctccaggcca gcagccgttt gatatcctgt tccgccagcg catacagcac gcccaggagc
    26161 gcggagattg cgccgatcgc catcaccaga atgccccacc acagcggcaa acccgtttgc
    26221 gccagcaggt ccatcgctac tttcaggatg ccgaaaatac cgattttgac cattacgcca
    26281 gacatcaacg ccgaagcgtg cgatggtgcg gcagggtgag cgcgcggcaa ccagctgtgc
    26341 aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa caccgccgac
    26401 gccagccccg gagaaagtga aagcgtgcgg aaactggcaa aatcgaggct gccgctttcg
    26461 cgccccatca gcaagaaggc gatcataatc agcaccgatc cggcgtgggc gataaaaaag
    26521 tagagcatgc cagcgttgat cgacgtttta tcctgcctgg caatgaccag aaaccaggaa
    26581 gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat caccagcagg
    26641 gcaaccatcg atgcgatgaa aagattcata aagaagccca tcgccgccgc gcctttgccc
    26701 tcgtattcgc gcatataagt caatgaatag agcgaacaaa ccaccaccag caaggagata
    26761 accagcacca taaatgccga gaggctatcc attcgcaggg taaaatcggc aaagtcaaaa
    26821 ggggtggcaa aacgggcgac taaagttaca ccagcatgta attgcgtaat tccgctgatg
    26881 atgccaatca cgccgcccac cagcgatgtg atgccggaaa gcttaatagc cagtctgtcc
    26941 agaccgagta aaaacagcga agccagactg gcaaacagat agaggatcag cgaccaggtt
    27001 aataattgca gggcatccat tagcgttgct cctgagtgag ggaaagggga tcctccccgc
    27061 cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt catctgtcgt tgcaggctat
    27121 cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg aacgcaggcc ggaccttctg
    27181 gcaagaaatc acacaggtcg catttcaccg cgacggtctg gacacccggt tcccagcgca
    27241 gcaatgcatg ttggggcggc acgttttctt cgccgtcttt gagtgagcct tcagcctgaa
    27301 aaacatattg cgcaggggca ttcaccggac ggcttcctga agcgctgatt gcgccaaatg
    27361 ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct ttcgttgagt tggatcgcat
    27421 catccctctg ggagatggca ttaaccgggc agacctgcag gcaaggggct tcctcacagt
    27481 gatgacacac gacaggggca gtgagtgttg acgtcttcgc cagggccagg cgcgggtgtt
    27541 gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc gagacaggta ttacatcctg
    27601 tacaccacag tggttcggcc accacaaagc ggttcatgtc ggctccttga aaatgatatg
    27661 aaacattgca tttcatattg cataaaggat gccattctga ttaactcaat atattcaata
    27721 agttatgtat tgactggatt ctgaacggta caaaaatgat ggtcattaac gttcagcaaa
    27781 aaatggattc gacacccgtg tcgcagggga aagcgagaat gtgatcttga tcacgagaag
    27841 tgaggtatgc tggccgcaag cgcagccagc acggaatgga gcaaagtaat caggcgtgtt
    27901 ctttcagcca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg tcaaagacat
    27961 gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatgcca tcgtaggttt
    28021 tgcccatgaa ggacttttca ccccagacgc cgaattgttc gcacacctgg tggtcctcat
    28081 cagataggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt ttttcgggtt
    28141 tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc atgttatcgc
    28201 gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag aaataaacca
    28261 gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga tccggcaagc
    28321 taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc catcctgttc
    28381 atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt ctgtacatag
    28441 ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt gggcggttat
    28501 ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt tgcgtgcgcg acaccagctc
    28561 cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga
    28621 gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg gacgcgccgt
    28681 cgtgcgcttc atcacgatta aaagatccag ttcggcacct ttcaacggta acgttgattc
    28741 aatcagagta atggcattcc atgaaccgga aagcagcata ataaacgtga actcttctcc
    28801 cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat gacgggtgat
    28861 ggtgttcaca attccagggc gatcggcacc caacgcagtg atcaccagat aatgttgcga
    28921 tgacagtgtc aaactggtta ttcctttaag gggtgagttg ttcttaagga aagcataaaa
    28981 aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc cataccaaac
    29041 gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa gtattgtcgc
    29101 gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat
    29161 tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca ctggcgagtc
    29221 cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg agctggctga
    29281 tgggcgcatt ccggtaattg ccgggaccgg cgctaacgct actgcggaag ccattagcct
    29341 gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc cttattacaa
    29401 tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgagc atactgacct
    29461 gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc cggaaacggt
    29521 gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag ggaacttaac
    29581 gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga gcggcgatga
    29641 tgcgagcgcg ctggacttca tgcaattggg cggtcatggg gttatttccg ttacggctaa
    29701 cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc attttgccga
    29761 ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg tcgaacccaa
    29821 tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg atacgctgcg
    29881 cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca
    29941 tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcaa aagtcgcgcc
    30001 tggcaaagat tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt tctgactcac
    30061 gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg cttgcggagc
    30121 ttcatgcccc ggctggaatg attttgccgg tgacctccgg tgattatgca atcccggtga
    30181 ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc cagccgctgg
    30241 cactggtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg ctggtggaaa
    30301 atggtcgtgg caatactctg tggccgcagg tggttagcgt gctgcaggcg aaaaactaca
    30361 ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta caatggaacc
    30421 gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt
    30481 atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa ccggttgcag
    30541 acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc gccggtctgg
    30601 ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc actatggacg
    30661 tacaaagtgc agctgatgac accggtttac caatgctggt cgtacgcggg ccgttcaatg
    30721 tggtttggca acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg accgacagca
    30781 cccgttcgca gggcaacatg gccgtaactt ataagccgct gtctgacagc gactggcagg
    30841 aactgggcgc gagcgatcca ggcctggcat ccggtgacta taaactgcag gttggcgatt
    30901 tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg actcagagtc
    30961 agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa tacagggctg
    31021 gaatcatccg gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacgc
    31081 gattttagcg gtaattcgca cgaaatttgt ttgtcggacg tagttcggat aaggcgttca
    31141 cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa acgttaattc
    31201 acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta aagcgaaaac
    31261 cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc
    31321 aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca acaagttcaa
    31381 ttacttcatt atgagcaaac tggctgaagc gggtatcccg actcaaatgg agcgtctgct
    31441 ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt gtgtcgtgcg
    31501 taaccgtgct gctggctctc tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa
    31561 cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga tggtcaacga
    31621 atcttactgc gaaacctttg gctgggtgag caaagagaac ctggcgcgta tgaaagagct
    31681 gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc tgattctggt
    31741 cgacttcaag ctggagtttg gcctgtacaa aggtgaagtg gtactgggcg atgagttctc
    31801 cccggacggc agccgcctgt gggacaaaga aacactggag aaaatggaca aagaccgttt
    31861 ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc gtcgcccgcc gcctgggtgt
    31921 acagctggac tgatttttct ggttgcttgt ttctttagcc ggagggtact ccggctaaat
    31981 gtctttgata cccccgctac aatacttctc attatctccc tttgacagga aagtcatacc
    32041 aggccaatat gtatatacat aacgtatatc ccaaaaaggg gggggctatg gaaactactg
    32101 tatttttaag taatcgcagt caggccgtca gattaccgaa agcggtcgct ctgccagaag
    32161 acgttaagaa agtagaaatt attgcgattg gtcgtacaag aatcatcact cccgccggtg
    32221 agtcctggga cagttggttt gatggcgaaa atgtgagtgc ggattttatg gatatccgtg
    32281 atcaacccgc aatgcaggaa agggaatcat tttgatgctg aaattcatgc tggataccaa
    32341 tatctgtatt ttcactatta agaataaacc agtacatgtc agagagcggt ttaacctgaa
    32401 ttctggccgg atgtgtataa gttctgtaac attgatggag cttatctatg gggcagaaaa
    32461 aagtcagatg ccagaacgca atttggcggt tattgaaggt ttcgtatctc gtttggtagt
    32521 gcttgattat gatagtgctg ctgcgacgca taccgggcaa atcagggccg agcttgcccg
    32581 gcagggaagg cctgttggac cttttgacca gatgatagcg ggacatgctc gcagtcgcgg
    32641 tttgattgtg gttaccaaca atacccgaga gtttgagcga gtggcgggaa tcagactaga
    32701 agactggagc taacattctc ttttacttct aacattcgta aattatataa cgtggcatga
    32761 agccttaatt cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc
    32821 tacgatcatt tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt
    32881 gaaagtgaca atgttgaaga caggcgcaac agctccggtg gtccgtctat gggcggtccc
    32941 ggttttcgcc tgccaagcgg taaaggcggg ctgattttac tgttagtcgt gctggttgca
    33001 ggctactatg gtgttgattt aaccgggtta atgactggac agccggtttc ccaacaacaa
    33061 tcaacgcggt ccattagccc taatgaagac gaagctgcaa aattcacctc ggtgattctg
    33121 gcaaccacgg aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa
    33181 ccgaagctgg tcatgtaccg tggaatgacg cgcaccggct gcggggctgg tcagtccatt
    33241 atggggccgt tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat
    33301 gacatgaaag acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat
    33361 gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa
    33421 caaaacgcga cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac
    33481 tgttttgccg gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aaccggcgat
    33541 ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt
    33601 caggggcgag tagtaccaga tagtttcaca catggcactt ctcagcaacg ctacagctgg
    33661 tttaaacgtg gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt
    33721 taacttccgg ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga
    33781 aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc
    33841 atgcgcttaa gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag
    33901 atgctgaaaa ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc
    33961 atgcggtatt cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa
    34021 cgttgaaagc gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc
    34081 aacctgatgc cgattcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt
    34141 ttgtccagca tctcaaacgc gtacttacgg cggataacga tgctatcctc tggcggcaaa
    34201 accagccgtt ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg
    34261 caccacaacc agaacaacag caactcttac agcagctact gaccatgcct ccgggcgtgg
    34321 cagcggtaac ggctgcgcgt gggcgcggta agtcggcgtt ggcagggcaa ctcatttctc
    34381 gtattgcggg cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac
    34441 aatttgcggg cgagaagttt cgctttattg caccggatgc cttgttagcc agcgatgagc
    34501 aagccgactg gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac
    34561 tggtatcgcg ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcaccg
    34621 gacgtggttt tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc
    34681 aacagccgat ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg
    34741 tttttgacga tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg
    34801 aacaaacgtt atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg
    34861 gtgcgcacta ccggacttcg ccgctggatt tacgccggat gatggatgca ccagggcaac
    34921 attttttaca ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg
    34981 ggggattatc tcaacaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta
    35041 atctggtggc ccagtcgctg gcggcgcacg gtagcaatcc actggcagcg acattgcgtg
    35101 gacggcgggt cagccggata gcagttcatc cggcgcgtca gcgggaaggc acagggcagc
    35161 aacttattgc tggtgctttg caatatacgc gtgacctcga ctatctttcg gtgagttttg
    35221 gttacaccgg ggagttatgg cgtttctggc accgctgcgg ttttgtgctg gtgcggatgg
    35281 gtaatcatcg tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagta
    35341 atgcgggtaa acaactggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc
    35401 tcgcaaagtg gaatggcgaa acgcttcctg ttgatccact aaacgatgcc gtcctttctg
    35461 acgacgactg gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacatcgt
    35521 taggttgctt attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc
    35581 gtttacagaa aaacgtcagc gacgcgcagt tatgtaccac acttaaactt tcaggccgca
    35641 agatgttact ggtccgtcag cgggaagagg ccgcgcaggc gctgttcgca cttaatgatg
    35701 ttcgcactga gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc
    35761 aattttctcc catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag
    35821 ttcgccatga aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg
    35881 ttactgcttt tccacggtgt cggggataac ccggtggcaa tgggggaaat aggtagctgg
    35941 tttgcaccgc tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt
    36001 aatccggcgg ggcgtcagtg gttttcagtg cagggtatta cggaagataa tcgccaggcg
    36061 cgtgtggatg ccatcatgcc gacgtttatt gaaacggtac gctactggca gaaacagagc
    36121 ggagtagggg ctaacgccac ggcgctaatt ggtttctcac aaggcgcgat tatggcgctg
    36181 gagagcatta aagccgaacc gggccttgct tcgcgcgtca ttgcctttaa cggacgctat
    36241 gcgagcctgc cggagaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa
    36301 gatccggtta tcgatctggc gcatgcagtt gcggcgcagg aagcgttaat cagtgccggt
    36361 ggtgacgtga cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg
    36421 caatttgccc tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta
    36481 agtggcggta agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg
    36541 gcctgataag acgcgtgagc gtcgcatcag gcatcgttca ccattgccgg atgtggcgtt
    36601 aacgccttat ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg
    36661 gccagtcgtc gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct
    36721 tgttggcgag aaatttcttg tggtcgacgc gcttgagatc tttaatcacg ttcagcagga
    36781 cgcctaccaa aaagacaatc accagaatcc accaatattt agccagccag tccatgctta
    36841 tttcctcttg cagaaccact catcaggcga cgagctgttc catgatacgt tgatacatac
    36901 gggcaagtag ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat
    36961 tgaccggccc gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg
    37021 ttccgcctgt ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga
    37081 ccgcatccac cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccagtcca
    37141 ccgtataacg cagttgatgt ttttcaagca gggcaagcac ctgtgctttg atcatctcat
    37201 cagtcagttc ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacat
    37261 tgttactgcc cgttcccgcc tgaatattag caatctgcat actggtcgcc gggaaaaatt
    37321 cattgccctg atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta
    37381 ctggattgtc agccagatgc gggtaggcca catgcccctg aacgccatga atggtaaggt
    37441 tgcaggttaa cgatccgcga cgaccatttt tcaccacatc accaaccact tcgatactcg
    37501 acggttcgcc gaccaggcag taatcgagac gctcattacg tgccatcaac gcttcgacga
    37561 cttttaccgt accgttgtgg gcactggctt cttcatcaga ggtgatcaga aatgccagtc
    37621 gccccgtatg gttgggatgt tgtgcgacaa agcgttctgc cgctaccacc atcgccgcca
    37681 gcgagccttt catatctgcc gcaccgcgtc cgaataacat gccatcacga atggtgggtt
    37741 caaacggcgg attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg
    37801 caaaggctaa cgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt
    37861 ccatgcgttc aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc
    37921 ctgcgtcatc aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg
    37981 ggcacgacat agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca
    38041 gcagcatagg cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag
    38101 tcattaatgc cgccgcagag gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt
    38161 tacgccaggt tgtaccacgg gtgttcagta acgcttccca gcctaattcg ttgataaaac
    38221 cgttcaataa ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt
    38281 tattggcttc cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa
    38341 gtgtaaccat aatgaatcct ttttcgattt atgagaataa atactcattt a
//
LOCUS       DAZGCS010000042        37559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGCS010000042 DAZGCS010000000
VERSION     DAZGCS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.042"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHK01_002189"
     CDS             complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHK01_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO8097137.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(581..1690)
                     /gene="apbC"
                     /locus_tag="ACHK01_002190"
     CDS             complement(581..1690)
                     /gene="apbC"
                     /locus_tag="ACHK01_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO8097138.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIVSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPGLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1822..3855
                     /gene="metG"
                     /locus_tag="ACHK01_002191"
     CDS             1822..3855
                     /gene="metG"
                     /locus_tag="ACHK01_002191"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO8097139.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3996..7790
                     /locus_tag="ACHK01_002192"
     CDS             3996..7790
                     /locus_tag="ACHK01_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO8097140.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7800..11432
                     /gene="yehI"
                     /locus_tag="ACHK01_002193"
     CDS             7800..11432
                     /gene="yehI"
                     /locus_tag="ACHK01_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO8097141.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARLGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11493..11813
                     /gene="yehK"
                     /locus_tag="ACHK01_002194"
     CDS             11493..11813
                     /gene="yehK"
                     /locus_tag="ACHK01_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO8097142.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            12996..14084
                     /gene="yehL"
                     /locus_tag="ACHK01_002195"
     CDS             12996..14084
                     /gene="yehL"
                     /locus_tag="ACHK01_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO8097143.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYTLLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14095..16298
                     /gene="yehM"
                     /locus_tag="ACHK01_002196"
                     /pseudo
     CDS             14095..16298
                     /gene="yehM"
                     /locus_tag="ACHK01_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            16291..17427
                     /gene="yehP"
                     /locus_tag="ACHK01_002197"
     CDS             16291..17427
                     /gene="yehP"
                     /locus_tag="ACHK01_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8097144.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17424..19424
                     /gene="yehQ"
                     /locus_tag="ACHK01_002198"
     CDS             17424..19424
                     /gene="yehQ"
                     /locus_tag="ACHK01_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO8097145.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19549..20010
                     /gene="yehR"
                     /locus_tag="ACHK01_002199"
                     /pseudo
     CDS             19549..20010
                     /gene="yehR"
                     /locus_tag="ACHK01_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(20051..20521)
                     /gene="yehS"
                     /locus_tag="ACHK01_002200"
     CDS             complement(20051..20521)
                     /gene="yehS"
                     /locus_tag="ACHK01_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO8097146.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20568..21287)
                     /gene="btsR"
                     /locus_tag="ACHK01_002201"
                     /gene_synonym="yehT"
     CDS             complement(20568..21287)
                     /gene="btsR"
                     /locus_tag="ACHK01_002201"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO8097147.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(21284..22969)
                     /gene="btsS"
                     /locus_tag="ACHK01_002202"
     CDS             complement(21284..22969)
                     /gene="btsS"
                     /locus_tag="ACHK01_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO8097148.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            23191..23922
                     /gene="mlrA"
                     /locus_tag="ACHK01_002203"
     CDS             23191..23922
                     /gene="mlrA"
                     /locus_tag="ACHK01_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO8097149.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDILPLGI"
     gene            23982..24089
                     /gene="yohO"
                     /locus_tag="ACHK01_002204"
     CDS             23982..24089
                     /gene="yohO"
                     /locus_tag="ACHK01_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO8097150.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(24070..24801)
                     /gene="yehW"
                     /locus_tag="ACHK01_002205"
     CDS             complement(24070..24801)
                     /gene="yehW"
                     /locus_tag="ACHK01_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO8097151.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(24806..25732)
                     /gene="yehX"
                     /locus_tag="ACHK01_002206"
     CDS             complement(24806..25732)
                     /gene="yehX"
                     /locus_tag="ACHK01_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO8097152.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(25725..26882)
                     /gene="yehY"
                     /locus_tag="ACHK01_002207"
     CDS             complement(25725..26882)
                     /gene="yehY"
                     /locus_tag="ACHK01_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO8097153.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(26889..27806)
                     /gene="osmF"
                     /locus_tag="ACHK01_002208"
     CDS             complement(26889..27806)
                     /gene="osmF"
                     /locus_tag="ACHK01_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO8097154.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(28017..30314)
                     /gene="bglX"
                     /locus_tag="ACHK01_002209"
     CDS             complement(28017..30314)
                     /gene="bglX"
                     /locus_tag="ACHK01_002209"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO8097155.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLINS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30510..32225
                     /gene="dld"
                     /locus_tag="ACHK01_002210"
     CDS             30510..32225
                     /gene="dld"
                     /locus_tag="ACHK01_002210"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8097156.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(32263..33195)
                     /gene="pbpG"
                     /locus_tag="ACHK01_002211"
     CDS             complement(32263..33195)
                     /gene="pbpG"
                     /locus_tag="ACHK01_002211"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO8097157.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATAASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(33369..33956)
                     /gene="yohC"
                     /locus_tag="ACHK01_002212"
     CDS             complement(33369..33956)
                     /gene="yohC"
                     /locus_tag="ACHK01_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO8097158.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            34126..34704
                     /gene="yohD"
                     /locus_tag="ACHK01_002213"
     CDS             34126..34704
                     /gene="yohD"
                     /locus_tag="ACHK01_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8097159.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVAFGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(34834..35595)
                     /gene="yohF"
                     /locus_tag="ACHK01_002214"
     CDS             complement(34834..35595)
                     /gene="yohF"
                     /locus_tag="ACHK01_002214"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8097160.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(35648..37084)
                     /gene="mdtQ"
                     /locus_tag="ACHK01_002215"
     CDS             complement(35648..37084)
                     /gene="mdtQ"
                     /locus_tag="ACHK01_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO8097161.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAGSNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            37308..37391
                     /gene="yohP"
                     /locus_tag="ACHK01_002216"
     CDS             37308..37391
                     /gene="yohP"
                     /locus_tag="ACHK01_002216"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO8097162.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         9248 a         9998 c         9460 g         8853 t
ORIGIN      
        1 cggctttcta aatgcaatta aaatcgggtt gccaaattaa tcgtagcgat ttaacacgat
       61 tcgacgtttg ctatcgctac ctgcaacaac cacttctgtc gttacacctt tgacgggcga
      121 agaaaacaga tgacgggtgt caacattata cgttaactga ccacaatgct gttgaggttg
      181 accattaacc atagccatac tgcatgttgg gggactgatc ctgccattaa tcgctaatgt
      241 ggcagtctct gaagagaatg cgggagaagc caatccatag gcaagaaaaa taattcctga
      301 cagccaatac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg
      361 agtgcataac aaactatagc tgtacatcca ctacacagcc acgaaggatg ataatgaagc
      421 attgcctgta tgatcaatcg actttgtaga gtttcggacg aaggtccgca gaatattcgc
      481 agtattaaat aagtgttcat taatgcggca aaaactaata caccgcatca atgtaacatc
      541 tctgcggtgt atgaagaata accagaatgg ttaataggcg ttagaccgcg cggaaggaaa
      601 tctcgcctgg aatgacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct
      661 ggcggtagat agcggtaaat tcgctctccg gacggctgat aaccgttggc gtgcctttat
      721 cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgt gtgtggtatt
      781 tttcagccag tttctcagca ccaccggtac cgaaaatcgg ttcatggtga ccacagttac
      841 tgcaaatatg cacactcatg ttctcgacaa taccaagcac cggaacctcg actttctcga
      901 acatcacaat gccttttttc gcatcgatca acgcgatgtc ctgcggggtg gtgaccacga
      961 ctgcaccagt tactggaatg ttttgcgcca gcgtcaattg aatgtcaccg gtgcctggcg
     1021 gcatatcaag caccagatag tcgagacccg gccacaaggt ttcctgcaac atctgcatta
     1081 acgccttgct cgccatcggt ccacgccaca ccatcgcatt gtcgtcagtg accagataac
     1141 caattgaatt ggttgccagg ccatgagaca caatcggtgc catatgggta ccgtccggcg
     1201 aagttggacg ctgattttct gcgcccagca tggtgggaat tgatgggccg tagatatcgg
     1261 cgtccagaat gccgactttc gccccttccg ccgccagtgc cagcgccagg tttaccgccg
     1321 tggaggattt acccacgccg cctttaccgg aactgaccgc aatgatgttc ttcacaccat
     1381 taatacccgg ctgattcttc acgcgtttta aggtggcgat attgtgcgac agtttccagt
     1441 cgatagcttt cgccccggtg atacgcagca gttcggcgct acattgctct tttaactctt
     1501 caaacgcgct atgccagacg aatggcataa ccagttccac atgcaacgtg tcgtccatcc
     1561 aggcgacatg gtgcaacgct ttcagcgtgg tgaggttatg cttcagggtt gggtgctgaa
     1621 aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt
     1681 gttcgttcat cccgactcct tttttgtata gataaaccat cagctgatag tttacctgaa
     1741 gaatatagag aagtacttac ttaacatttt cccatttggt actatctaac cccttttcac
     1801 tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca
     1861 ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat
     1921 gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat
     1981 gcccacggta caccgatcat gctgaaagct cagcagctcg gtatcacccc ggagcagatg
     2041 attggcgaaa tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac
     2101 aactatcact cgacgcacag cgaagagaac cgccagttgt cagaacttat ctactctcgc
     2161 ctgaaagaaa acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa
     2221 ggcatgttcc tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat
     2281 caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga attgatcgag
     2341 ccgaaatcgg tggtttctgg cgctacgccg gtaatgcgtg attctgaaca cttcttcttt
     2401 gatctgccct ctttcagcga aatgttgcag gcatggaccc gcagcggtgc gttgcaggag
     2461 caggtggcaa ataaaatgca ggagtggttt gaatccggcc tgcaacagtg ggatatctcc
     2521 cgcgacgcgc catactttgg ttttgaaatt ccgaacgcgc cgggcaaata tttctacgtc
     2581 tggctggacg caccgattgg ctacatgggt tctttcaaga atctgtgcga caagcgcggc
     2641 gacagcgtaa gcttcgatga atactggaag aaagactcca ccgccgagct gtaccacttc
     2701 atcggtaaag atattgttta cttccacagc ctgttctggc ctgccatgct ggaaggcagc
     2761 aacttccgca agccgaccaa cctgtttgtt catggctatg tgacggtgaa cggcgcaaag
     2821 atgtccaagt ctcgcggcac ctttattaaa gccagcacct ggctgaatca ttttgacgct
     2881 gacagcctgc gttactacta cactgcgaaa ctctcttcgc gcattgatga tatcgatctc
     2941 aacctggagg atttcgttca gcgtgtgaat gccgatatcg tgaacaaagt ggttaacctg
     3001 gcctctcgta atgcgggctt tatcaacaag cgttttgacg gcgtgctggc aagcgaattg
     3061 gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg
     3121 gaaagccgcg aatttggtaa agcagtgcgc gaaatcatgg cgctggctga tctggctaac
     3181 cgctatgtcg acgagcaggc tccgtgggtg gtggcaaaac aggaaggccg cgatgccgac
     3241 ctgcaggcaa tttgctcaat gggcataaac ctgttccgcg tgctgatgac ttacctgaag
     3301 ccggtactgc cgaaactgac cgagcgcgca gaagcattcc tcaatacaga actgacctgg
     3361 gatggtatcc agcaaccgct gctgggccac aaagtgaatc cgttcaaagc gctgtataac
     3421 cgcatcgata tgaagcaggt tgaagcactg gtggaagcgt ctaaagaaga agtgaaagca
     3481 gctgccgcgc cagttactgg cccactggca gatgatccga ttcaggaaac catcaccttt
     3541 gacgacttcg caaaagttga cctgcgcgtg gcgctgatcg aaaacgcaga gtttgttgaa
     3601 ggttctgaca aactgctgcg cctgacactg gatctcggcg gtgaaaaacg caatgtcttc
     3661 tccggtattc gttctgccta cccggatccg caggcactga tcgggcgtca caccattatg
     3721 gtggccaacc tggcaccgcg taaaatgcgc tttggtatct ccgaaggcat ggtaatggct
     3781 gctggtcctg gcgggaaaga tatcttcctg ttaagcccgg atgctggtgc taaaccaggc
     3841 catcaggtga aataatcccc ctttaaggcg ctgcatcgac agcgcctttt ctttataaat
     3901 tcctaaagtt gttttcttgc gattttgtct ctctctaacc cgcataaata ctggtagcat
     3961 ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc tatcaggacg
     4021 aaaaatcaca taaattctgg gcagttgagc aacagggaaa cgagttgcat atcagttggg
     4081 gaaaagttgg caccaaaggg caaagccaga taaaaagttt ttcagatgct gcggcagtgg
     4141 caaaagcgga gcttaagctg attgcggaga aggtgaagaa ggggtatgtg gagcaagcga
     4201 aggataattc tttgcaacct tcccaaacgg taacgggctc tctcaaggta gcggacttat
     4261 ccaccattat tcaggaacaa ccctctttcg tagcagaaac ccgtgcgcct gacaaaaata
     4321 cagatgctgt tttaccgtgg ctggcgaaag atattgctgt cgtttttccg cccgaagttg
     4381 tacacaccac gttaagtcat cgccgctttc ccggagttcc tgttcagcaa gcagacaaat
     4441 tgacccaatt acgtcgctta gcctgtagtg tgtcgcaacg ggataataaa acagccacat
     4501 ttgacttcag cgcctgttct ttagaatggc aaaacaccgt cgcccaggcg atcagtcaga
     4561 tcgacggcct gaaaacaaca cagctaccat caccagtaat ggctgtactc acggcacttg
     4621 aaatgaaatg cacaagatat aaagtgcgtg aggatgttat ggatcagatc gtccaggaag
     4681 gcggtctgga atatgctact gatgtaataa tacaccttca acagattgat attaaatggg
     4741 attatgcgaa taatgtcatt attattctgc cgtctggcat tgcacctgac tacttggagc
     4801 aatattccag atttgaatta cgcctacgta aacatttatc actggcggaa gagtctctct
     4861 ggcaaaaatg tgcacaaaaa cttattgccg caattccaca tattccagaa tggcggcaac
     4921 cattaattgc tttgttatta cccgaaaaac cagaaattgc acatgaaatt gcccagcgtc
     4981 tactggggca aaaaaaatta ccctcgcttg agtggttaaa aatagtggcg actgatgagc
     5041 acattcttgc ctcattagaa aaatatcacg agccatatgc catttttgat gattactatt
     5101 gtggtgcgat atggtcagcc accgtattac aggagcaagg tgttgcagcc ctgccccgat
     5161 ttgctcccta tgccgcaagt gactactgcg ccgatgtgtt gcgtcatatc aatcatccgt
     5221 tcgcattaac actgcttata cgtgtagccg ggcatactaa acgctgtcac gatcggatga
     5281 cgaaagcctg cgctgcgttc ccacatgcag caatggcggc actgacggaa cttcttgggc
     5341 aaaaagaaga gaacagttgg cgcattatgc taatgacaat gcttatctca caaccagcac
     5401 tggcagaaca ggtcattccc tggctctcga cacccgcagt tgccgtactg aaatcatgcc
     5461 agcaacaact gacacagccc tcaaaccatg ccagcgccga tctactgcca gccgtagtag
     5521 tctcccctcc ctggctttcg aaaaagaaaa aatcgccgat tccggtgctg gatttagcgc
     5581 cattaggcat tgagccaatc tgttatctga cagaagaaat cagtaatcaa cttttggcga
     5641 aatatatctg gtattcaaaa cacatcacgg ttagccatga agaaagtact accaacctgt
     5701 tggcaaggat gggttttcaa cgacggatcg ctggtacata tattaaagct cccgaagcgg
     5761 tagttgaggc atggctaaat gaagattatt caaccttact aagtgaattt aaggtgtttc
     5821 attcacctac cgggcattat tggcagttgg ggattttgac aacattgccg ctggagaaag
     5881 cagtaaaagc atggaatgcc cttaccctat ctccacatac cgataccgaa tacgccatgt
     5941 tacattttgg actcaaaggg ttacctgggt tagtaaactc acttgcacgc tatccacaag
     6001 aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc gtcgcccgtg
     6061 ccttcaacaa actgaaaacg ctacgcgaaa acgcccgtag ctggctgttg aaatacccgg
     6121 aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa gcacaggata
     6181 acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta ctgcaagaaa
     6241 tcgcccgacg ttataaccag ccggaagtaa ccgatgcggt gaacgctctg cttgcgctcg
     6301 atcccttaga taatcacccg acaaaaatcc ccactcttcc ggccttttat cagccatcgc
     6361 tctggacgcg cccggtatta aaagcaaatg cccaatcact gccagatagc gccctcctcc
     6421 acctcggtga aatgctccgc ttccctcagg aagaggctct gtatccggga ttattgcagg
     6481 tgaaagacgt ctgttccgcc gactcactgg cgggatttgc ctgggatctg tttaccgcct
     6541 ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttctcg
     6601 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
     6661 agcataaacg cgccaccgtt gggttggata ttctcgctgc tatcggtagt gatatcgccc
     6721 ttatgcagct taacggcatc gcccagaaac tgaaattcaa agcattacag gagcgggcaa
     6781 aagaaaaaat tgccgacatt gccgagagcc gcgaactcac ggtggcggag cttgaagatc
     6841 ggttagcacc ggatctcggt ctggatgata acggttcgct gctgctggat tttggcccac
     6901 ggcagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat gtttccggca
     6961 gccgcctgaa agacctgccc aaaccgaaca aaagcgatga tgaaacgcgg gcgaacgatg
     7021 cggttaaccg ctacaaattg ctgaaaaaag atgcgcgtac catcgccgcc cagcaggtag
     7081 caaggctgga atccgccatg tgcctgcgcc gccgctggtc gctggaaaac ttccagctct
     7141 tcctggttga gcatccgctg gttcgtcact taacccgccg tctgatttgg ggcgtttata
     7201 gcgccgaaaa ccagctactg gcttgctttc gcgtagcaga agataacagc tacagcaccg
     7261 ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcact cctcacgttc
     7321 tggaaatatc accaacggat gctgccgcct ttggtcagct ttttgccgac tacgaactgc
     7381 taccaccgtt ccgccagctc gaccgtaaca gctacgccct gacagaagcc gagcgcaatg
     7441 ccagtgaact gacccgctgg gcaggcagaa aatgcccgag cggtcgggtc atgggactgg
     7501 cgaataaagg ttgggtgcgt ggtgaaccgc aggatggtgg ctggatcggc tggatgatca
     7561 aacctctggg ccgctggtcg ttaatcatgg aaatcgatga aggctttgca gttggtatgt
     7621 cgccagccga acttagcgcc gagcaactct taagcaagct gtggctatgg gaaggcaaag
     7681 cagaaagcta tggctggggg agtaattcaa cacaggaagc gcagttctcc gtactcgatg
     7741 ccatcaccgc cagcgagctg attaacgata ttgaagccct gtttgaataa ggaaagagca
     7801 tggacaagga attaccgtgg ctggcggata acgcccaact ggaactgaaa tataaaaaag
     7861 gcaaaacacc actcagccat cgcaattggc cgggcgaacc agtgcctgtt atcactgaaa
     7921 gcatcattca gacactgggt gatgaattgc tacaaaaagc tgagaagaaa aaaaacattg
     7981 tctggcgtta tgagaatttt tcactggagt ggcagtccgc cattacgcag gccatcaact
     8041 tgatcggcga acacaaaccc tcaatcccgg cccagacaat ggcggcgcta gtctgtatcg
     8101 cgcaaaatga cagccaacag ttgctcgacg aaatcgtcca acaagagggg ctggaatatg
     8161 cgactgaggt ggtgattgca cgccagttta ttacacgatg ttatgagagt gatcctctgg
     8221 tagtcacatt gcagtatcag aacgaggact atggctatgg ttatcgctca gaaacctata
     8281 acgaattcga tctccgactg cgtaagcatc tctctctggc agaggaaagc tgctggcagc
     8341 gttgcgccga caaactcatt gccacactgc caggaataac caaagttcgc cgccctttta
     8401 ttgcgctgat cctccccgaa aaaccagaaa tcgccaatga gttggtaagc cttgaatgcc
     8461 cgcggactca ttttcattct aaggagtggt taaaagttgt tgctaatgac cccacagcgg
     8521 tgagaaaact cgaacgctac tggagccagg atatatttag cgatcgagaa gccagctaca
     8581 tgtcgcatga aaaccacttc ggctacgcgg cctgcgccgc ccttttgcgc gaacaaggac
     8641 tggcagccgt tccgcgcctc gcgatatatg cccataaaga agattgcggc agtctgctgg
     8701 tacaaattaa ccatccgcaa gtcatccgca ccttgctgtt agttgctgat aaaaacaaac
     8761 ccagcctgca acgtgtggct aaatacagta aaaacttccc ccatgcgaca ctcgccgcac
     8821 tggcagaact gctggcgtta aaagaaccac cagcccgcct aggttatcca atcatcgaag
     8881 acaaaaagct gcctgcacag caaaaagcac gcgatgaata ctggcgtacg ctgttacaaa
     8941 cgctgatagc atcgcagcca caactagcag aaagggtgat gccgtggtta agtactcaag
     9001 ccagggcagt gctgaatagt tatttatcgg caccgccaaa accggttatt gatagcaccg
     9061 ataacagcag tctgcctgaa atgctcgttt caccaccgtg gcgtagtaag aaaaaaatga
     9121 cagctccacg tcttgatttg gcaccgctcg aattaactcc gcaaatttac tggcaaccag
     9181 gcgaacaaga gaggcttgcc gccactgagt ctgcccgtta tttcagcacg gaatctcttg
     9241 cggaacgcat ggaacaaaaa agtggacgag ttgtattaca ggaactgggt tttggggatg
     9301 atgtatggct gtttctgaat tatatactcc ccggaaaact ggatgctgca cgcaattcac
     9361 tcattgttca gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact
     9421 ccccgcaagc acaattagca gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta
     9481 acatatggga aaatgacaac ttctcacgtt atcgtccgga aaagagtgtc tggaacctgt
     9541 atttattggc acagttgccg cgtgagatgg ctttgacctt ctggctgcgt atcattgaga
     9601 aaaagcatct gttcgcgggt gaggactatt ttctcagtat cctcggattg gatgcgctac
     9661 caggtctgct gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt
     9721 ttggcgcaac agaactggcg ctgcctgttg cccgcgtctg gcgacgtttc gcggcgcagc
     9781 gtgatctggc tcgccagtgg attttacaat ggccggaaca tacggctagt gcacttatcc
     9841 ctcttgtctt taccaaaccc agcgataata gcgaagccgc attacttgcc ctgcgtttac
     9901 tgtacgaaca gggacatggc gaattgctac aaaccgtggc aaaccgctgg cagcgtacag
     9961 atgtatggtc tgccctggag cagttgctta aacagggtcc aatggacatt tacccggcac
    10021 gcattccaaa agcccctgat ttctggcatc cggcaatgtg gtccaggcct cgacttatca
    10081 ctaataatca gcctgttacc ggtgacgctc tggaaattat cggcgaaatg ctgcgcttta
    10141 cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa
    10201 cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt gcccccgcaa
    10261 aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg gcacgggatc
    10321 tgacgacaca gatcctcgcc tggccacaag aaggcaaatc tgcccgtgct gtcagcggcc
    10381 tgaacatcct taccctgatg aataatgata tggcgctgat acagctgcat catatatcgc
    10441 aacgggctaa atcccgcccc ttacgtgata acgcggcgga atttcttcag gtggtcgcag
    10501 aaaatcgcgg gctaagccag gaagagctag cggacagatt agtcccaacc ctgggccttg
    10561 atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt cgcttcgatg
    10621 aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagc gttccccggt
    10681 tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta aaagggctaa
    10741 aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct gccctacgta
    10801 ccacccgacg ctggtcgctg gcagattttc atactctgtt tgttaatcat ccctttaccc
    10861 gcctggttac ccagcgatta atatggggcg tgtatccggc aaatgaaccg cgtcgtttac
    10921 tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa tgcgcaggat gagccaattg
    10981 acctgcctgc ggatgctctg attggcattg cccacccgtt agaaatgaca gcagaaatgc
    11041 gcagtgaatt tgcccagctt tttgccgatt acgaaattat accgccactt cgccagttga
    11101 cacggcgcac ggtgctgctc acgcctgacg agtcagccag taatagtttg aatcgctggg
    11161 aaggtaaatc tgctaccgtt gggcaactta tgggaatgcg atacaaaggc tgggagtcag
    11221 gctatgagga cgcatttgtc tatgacctgg gtgagtaccg gctggtcctt aagttttcac
    11281 ccggttttaa ccactacaat gttgatagca aagcgctaat gagcttccgt tctcttcgag
    11341 tgtacagtga caataaatcc gtcacttttg ccgaacttga tgtgtttgat ttgagtgagg
    11401 cgttaagcgc acctgacgtc attttccatt aaaacacagc gggcagtgat gcaactgccc
    11461 gttatcaaca cgacttacca gcggggaaag cgatgatcgt gcaaaaagag ctggttgcta
    11521 tttacgatta tgaggtccct gtacctgaag atccgttttc cttcagactt gagatccata
    11581 aatgctctga attatttaca ggttccgtct atcgactgga gcgattccgg ctacgtccaa
    11641 catttcatca acgtgatcga gaagatgctg acccgctaat aaatgatgcg ttgatttata
    11701 taagagatga gtgtattgat gagcggaaat tacgaggtga atcacctgaa actgtaatag
    11761 caatttttaa tcgtgaacta cagaatatat tcaaccaact aatagaatat tagtttatta
    11821 aagggggaag cggttttccc ccttagttgc gtttcaattt gcccattact tcaacaaatc
    11881 aatcaaccgt aacagagaca ctaatagctc cagtacggca atgagcacgg ttaacttttg
    11941 tgtcagcgtg tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac
    12001 actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc ggatcagagc
    12061 actacctgcg gcaccacccg tccggtagcc aggacttgaa atgggttcga tccggcgcaa
    12121 cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt ccagaggtgg
    12181 ttataattcg ttcgttgaga tgccatgttg cactatcagt aacttaacat caaagtagta
    12241 aataaaagaa cctgcctcac cagcaggttt ttttattttc tgcgatctac tttccagata
    12301 ttttttgcca atagcccacc ataaatattc acatcccggt caaatggtgc gttgaatata
    12361 aaatcgatga atttataaat aaaaatgagt tatatgatga attaataaaa acagtaatca
    12421 ttttattaaa gggggaagcg gttttccccc ttagatgcgt ttcaatttgc ccgttacttc
    12481 aacaaatcaa tcaaccgtaa cagagccact aatagctcca gtacagcaat gagcacggtt
    12541 aacttttgtg tcagcgtgtc catccgctct ccttattaag gagcgcaaca ccagcacctt
    12601 acccttacac tgcctgtcga tactggttta cgtatgttgg accacgatgt tgcactccgg
    12661 atcagggcac cacctgcagc accacccgtc cggtagccag gacttgaaat agattcgatc
    12721 cggcgcaacg tcccaacggt ctggattata aatctcatta tcttaattgc aacggggtcc
    12781 agccgtggtt ataattcgtt cgttggaaca cgatgttgca caggctgtgg tgttaggcct
    12841 gaaagtagta aataaaagaa cctgcctcac cagcaggttt ttttatttac tgtgatctgc
    12901 tttccagata tttttcgctc aaacaactaa tgcgccaaac atttattgcg cgtaaaatat
    12961 cgtttatttc attaatacat ttcagggatg aatatatgtc accgcagaat aatcatctgc
    13021 agcgtccgcc tgctgctgtg ttatacgccg atgaactggc aaaattaaaa caaaatgata
    13081 acgcaccttg cccgcccggt tggcagttaa gtttgcctgc ggcccgtgct tttatccttg
    13141 gcgatagcgc gcaaaatatt agccgtaaag tggtgattag tccctccgct gtcgaacgca
    13201 tgttagtcac tcttgccacc gggcgcggtt tgatgttggt gggggaacct ggcaccgcaa
    13261 aatctctcct gtctgaatta ctggcaaccg ctattagcgg cgatgccggg ttaaccatcc
    13321 agggcggcgc atctactacc gaagatcaaa tcaagtacgg ctggaactac accctgctta
    13381 tcaaccatgg tccgtcaacg gaagcactgg tccctgcccc gctttaccag ggaatgcgcg
    13441 acggcaaaat cgtccgtttt gaagagataa cgcgtacacc gctggaagta caagactgtc
    13501 tgttaggtat gctttctgac agggtgatga cggtgccgga actcaccggt gaagccagtc
    13561 agctctatgc gcgggaaggc tttaatatta ttgccactgc caatacccgc gatcgcggcg
    13621 tcaatgagat gagtgccgcg ctaaagcgtc gcttcgattt tgaaaccgtc tttccgatta
    13681 tggatttcgc ccaggagctg gaactggtcg ccagtgcttc ggcgcgtttg ctggcccata
    13741 gcggtattcc gcataaagtg cctgacgcag tactggagtt actggtacgc accttccgcg
    13801 atctgcgtgc caacggcgaa aagaaaacgt caatggatac actgacagcg attatgtcca
    13861 ccgctgaagc cgtgaacgtg gcccacgccg taggcgtccg cgcctggttt ttagcgaatc
    13921 gcgcggggga gcctgccgac ctggtggact gtattgccgg aaccatcgtc aaagataacg
    13981 aagaagatcg cgctcgtctg cgccgttact tcgaacagcg tgtcgccaca cataaagaag
    14041 ctcactggca ggcttattat caagcccgcc accgcctgcc gtgaggaaag aggcatgagc
    14101 gagccgttaa ttgtcggcat ccggcatcat agtccggcct gcgcccggct ggtgaaatcg
    14161 ttaatcgaaa gccagcggcc acgatacgtg ttgattgaag gcccggctga ttttaatgac
    14221 cgggtagacg aacttttttt tcccaccagc ttccggtagc tatttacagt tattgccagt
    14281 atcaggacgg tgcagccccc gggcgtggtg cctggacgcc atttgctgaa ttttcgccgg
    14341 agtggcaggc gctacaagcc gcacgtcgta ttcaggcgca aacttacttc atcgatttgc
    14401 cttgctgggc gcagagtgaa gaagaggacg attcgcctga tacgcaagat gaaagccagc
    14461 aaactgcatt accctctgcg ctggcgcact attttgccca actgcggggc gatttccccg
    14521 gcgatgcact caatcgtcag cgcgaagcct ttatggctcg ctggattgca tgggcggtgc
    14581 agcaaaataa tggcgacgtg ttagtcgtct gcggtggctg gcacgctccg gcactggcaa
    14641 aaatgtggcg cgaatgcccg caggacatta acacgccgga attgccctcg ctggcagatg
    14701 ccattacagg ttgttatctc acgccctaca gtgaaaagcg ccttgatgtg ctggcaggat
    14761 acctttccgg tatgcctgcc ccggtctggc aaaactggtg ctggcagtgg ggcttacagc
    14821 aggccggtga acaactgcta aaaactatcc ttacccgttt gcgccagcac aagttgcctg
    14881 cttcgaccgc ggatatggct gccgctcatc tgcatgcgat ggcgctggca cagttgcgcg
    14941 gtcatacact accgttacgc actgactggc tggatgccat agcaggatcg ctgattaaag
    15001 aagccctgaa tgcgccgttg ccgtggagct atcgcggcgt tattcatccc gacaccgatc
    15061 cgattctgct aacgttgata gacacattag cgggtgacgg attcggtaaa cttgcccctt
    15121 ctacgccaca acctcctctg ccaaaagatg tcacctgcga actggaacgt accgcaatct
    15181 cccttccggc ggagcttacc ttaaatcgct ttaaccccaa tggactagcg caaagtcagg
    15241 tgttacatcg gctggcaata ctggagatcc cagggattgt gcgccagcag ggaagcacac
    15301 tgacacttgc aggcaacggt gaagaacact ggaaattaac ccgcccgctt agccagcatg
    15361 cggcattgat tgaggccgcc tgctttggtg ccacactcca ggaagccgca cgccataaat
    15421 tagaagccga tatgctggac gcgggtggaa tcggcagtat caccacatgt cttagccagg
    15481 cggcgttagc gggtctggcg tccttcagtc aacaattact ggagcaactc acattattaa
    15541 tcgcccagga aaatcaattt gccgaaatgg gccaggcgct ggaagtgcta tatgccttat
    15601 ggcggctgga tgaaattagc ggtatgcaag gcgcgcagat attacaaacg acgttatgcg
    15661 cggctatcga tcgcacgctg tggctgtgtg aatctaacgg cagaccggat gaaaaggagt
    15721 ttcacgctca cctgcatagc tggcaagcgc tttgccatat tctgcgcgat ctacatagcg
    15781 gcgttaattt atccggcgtt tcgctttctg cggcggtagc cttactggag cgacgcagtc
    15841 aggcaattca tgccccggcg ctggatcgcg gcgcggctca tggcgcactg atgcgtctgg
    15901 aacatcctaa cgccagtgcc gaagcggcgc tgacgatgct ggcgcagtta tccccggcac
    15961 aatccggcga agcgctgcac ggtttgctgg cgctggcccg ccatcaactg gcctgtcagc
    16021 cgacatttat cgccggtttc agcagtcatt taaatcaact aagtgatgcc gattttatca
    16081 atgccctgcc cgatttacgc gcggcgatgg cctggctacc accacgagaa cgcgggacgc
    16141 tggcgcatca ggtgcttgag cattatcaac tggcgcaact tcccgtttcg gcgctgcaaa
    16201 tgccgttgca ttgtccacca caggccattg cacatcatca acaactcgaa cagcaggcac
    16261 tggcatcgct gcaaaactgg ggagttttcc atgtctgaac tgaacgatct tctgaccacc
    16321 cgtgagctac aacgctggcg attaattctt ggcgaagcgg cagaaacgac gctttgtggg
    16381 ctggatgaca acgcccggca gatagaccac gcgctggagt ggctgtatgg gcgcgatcct
    16441 gaacggctcc agcgtggtga acgctctggt ggattaggtg gctcaaatct caccacccct
    16501 gagtggatca acagtattca cactctgttt ccgcaacagg tgattgagcg actggaaagc
    16561 gatgccgtac tgcgctacgg cattgaagat gtggtgacga atctcgacgt gctggaacgt
    16621 atgcagcctt cggaaagcct gctacgcgcc gttttgcaca ccaaacatct gatgaatccc
    16681 gaagtactgg ctgccgcccg ccagatagtg cgccaggttg ttgaagaaat tatggcgcga
    16741 ctggcaaagg aagttcgtca ggctttttct ggtgtccgcg atcgccgtcg ccgctcatta
    16801 attccactgg cgcgaaactt tgatttcaaa agtactctgc gcgccaacct gcaacactgg
    16861 cacccgcaac acggcaagtt gtatatcgaa tccccccgct ttaacagccg cattaaacgc
    16921 caaagcgaac aatggcaact ggtcttactg gttgatcaaa gcggatcgat ggtggactcg
    16981 gtgatccact ctgcggtgat ggcggcctgt ttgtggcagt tacccggcat tcgtacccat
    17041 ctggtggcgt ttgacacaag cgtggttgat ctcacggcag acgttgccga tccggtagag
    17101 ttattaatga aagtacagtt gggcggcggg accaatatcg ccagtgccgt ggagtatggt
    17161 cggcaactta ttgaacaacc agcaaaaagc gtcattatcc tcgtgagcga tttttacgaa
    17221 gggggttcat catcattgct gacgcatcag gtaaaaaagt gtgtccagag cggcatcaaa
    17281 gtgctgggac tggcagcgct cgatagcacc gcaacgcctt gctatgaccg cgatacggcc
    17341 caggcgctgg ttaatgtcgg cgcacaaata gccgccatga cgccgggcga gctggcatca
    17401 tggcttgcgg agaatcttca gtcatgaatt cactacgtcc ggaattatta gaactgacac
    17461 cgcaggcact gacggcgtta agcaatgccg gttttgttaa gcgcagtctt aaggaactgg
    17521 aaaatggcaa cgtcccggaa atcagccatg agaacggcgc tttaatcgcc accttcagtg
    17581 acggtgtccg tacccagctg gcgaacagcc aggcactgaa agaggctcag tgcagctgcg
    17641 gggccagcgg catgtgccgt catcgcgtga tgctggtgtt aagttatcaa cgactttgtg
    17701 ccaccgtcca gcccacggaa aaagaagaag agtgggatcc ggcaatctgg ctggaagaac
    17761 tggctaccct gcccgatgcc acccgcaaac gcgcgcaggc gctggtcgct aaaggcatca
    17821 ccattgagtt gttctgtgcg ccgggcgaaa ttccctctgc ccgcttaccg atgagcgatg
    17881 tgcgttttta ttcccgtagc agtattcgtt tcgcccgttg tgattgtatt gaaggcacac
    17941 tttgcgaaca tgtcgtactg gctgtgcagg ccttcgtcga ggccaaagcg cagcaagcag
    18001 aatttaacca tttaatctgg cagatgcgca gcgaacacgt cacatcatct gacgatccgt
    18061 ttgccagcga agaaggccaa acgtgtcgtc aatatgttca gcaattaagc caggcattat
    18121 ggcttggcgg catcagccag ccgcttattc actacgaggc agcattcaac cgcacattgc
    18181 aggcggcaga ggcctgcaac tggcgctggg tgagtgaatc actacgacag ctacgggcaa
    18241 gcgttgatgc cttccacact cgcgccagcc actatcatgc cggagaatgc ttacgtcagc
    18301 ttgcggcatt aaacagtcga ttaaattgcg cacaagagat ggcccggcgc gacagtgttg
    18361 gtgaagttcc tcctgtgccg tggcgcacgg tcgttggctc tggcattgcc ggagaagcaa
    18421 agcttgatca tctgcggctg gtgtctttag gtatgcgttg ctggcaggat attgagcatt
    18481 atggtttacg catctggttt accgatcccg acaccggcag tattttgcac ctttcgcgca
    18541 gttggccgcg aagtgaacag gaaaactcac cggcagctac gcgtcggctg tttagttttc
    18601 aggctggcgc actggcgggt gggcaaattg tttcacaagc agcaaaacgc agtgccgatg
    18661 gcgagctgct gttagctacc cgcaaccgct taagcagcgt tgtgccgctg tcgcctgatg
    18721 cctggcaaat gttgagcgcg ccgttacgcc agccgggcat tgtggctttg cgggaatatt
    18781 tacgccagcg tccccccgcc tgcatacggc ctcttaatca ggtcgataac ttatttattc
    18841 tgccggtcgc tgagtgtatt tcgctcggtt gggatagcag ccgccagacg ctggatgcgc
    18901 aggtaatcag cggcgaaggt gaagataatc tcctgacgtt atcccttccg gcatctgcct
    18961 gttctccttt tgccgttgaa cgcatggcgg cgcttttgca acaaacagac gaccccgtga
    19021 gtctggtttc tggctttgtc agttttgttg atgggcaatt gacactggaa ccacgggtga
    19081 tgatgacaaa aacccgcgcc tgggcgctgg acgcagaaac tgcgcctgtg gcaccgctac
    19141 cttctgccag cgttttgcct gtgccgtcta ccgctcatca gttgctgatg cgctgccagg
    19201 cgttacttat tcaactgctc cataacggct ggcgctatca ggaacagagc gctattagtc
    19261 aggcagagtt gctggcgaat gacctctccg cggtcggttt ttatcggctg gcacatgtgt
    19321 tgggacaatt tcgtaataca gaaagcgagg cacgggtaga agcaatgaat aacggtgttt
    19381 tactttgcga acaattattc cccatgcttc agcaacaagg atgaaatagt gctttttact
    19441 aagagttcta ctccagttcc ggactgctca cgtcacggta ttaggcatat tctatatagc
    19501 ccctggtgag agtcaccagt ttcttgatta aataaaatgg agttttacat gaaggctttc
    19561 aataagctgt tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt
    19621 gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc
    19681 tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgct
    19741 tctattggtg caaccaccaa agaagacgct gccaagacac ttgagccgtt aagcgccaaa
    19801 tacaaaaaca tcgcgggtgt tgaagaataa ttaacctata cagataccta cgcgcaggaa
    19861 aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc
    19921 aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg
    19981 aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttc gcatggccgc
    20041 catacccgtc ttagcttttc ttcacatgct ggcgcgctgc cagtccacgc agaaaataac
    20101 gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg
    20161 taatttccgg catcgaaacg cggaactgct gttcggtgag gatagccaga atgtcatcgg
    20221 ttttcagcga aaacgcgatg cgtaattttt tcagcacgat gttgttatta atgcgacgtt
    20281 ccggctccag tgccggagca gactcatcct tgccgcgttt ttcataaatc aggccattga
    20341 ggaatgacga caaaacaatg tccggacaac gctgaaaacc ctcttcgtct tctttacgta
    20401 gccagacggc gatctgttcc gcggtggctt cgacattacc cagcgccaga atacgcacca
    20461 ggtcattatt attggctttc aaaatgtagc gcacgctgcg cagaatatcg ttacttagca
    20521 tgaggccttc aggtgttgat gaggcaaaaa gccattttag cagtctttta caggccaatc
    20581 gcctctttta agcttttcag ataacggcgg ctgaccggca cggttaagcc attacgcaaa
    20641 atcaactcgg cctggccgtt atcttccaga cgaatctcct gtaaatgcgc gaggttaacc
    20701 agatactgac gatggcagcg cagtagtggt gtacgacttt ccagggtacg taatgtcaat
    20761 tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga caccgctcat ccgactgctg
    20821 acaaatgcca catctttcat ttgcagcaaa taaatccgac tatgccccgt acaagggata
    20881 aatttcagcg cctgttgatt ttccggtaac agcgaaacat cctgcttgct gcgctcctga
    20941 cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag cagataatca
    21001 aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag aaaaacaata
    21061 tacgggcgat gttccgggtc aagcatccca accatttcca gaccactgat gcgcggcatc
    21121 tggatatcga gaaacagcac atccgggcgc agtttatgca ccgcgccgat cccttccacg
    21181 gcgtttgaac actctccaac gatttcaata tcgctctgct cctgcaaaaa tacacgcagg
    21241 ttctcccgtg ctaacggttc atcatcgaca attaagactt taatcatgcc tcgtccctcc
    21301 atggtagtcg taacgttatt cgggtgtaac tatcaggctc acaggcgacg cttattccat
    21361 agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg
    21421 cattggttac cggttgatac aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc
    21481 cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt
    21541 taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt
    21601 cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca
    21661 gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta
    21721 agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc
    21781 ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgagga ttcacctggg
    21841 cgtgaagcag tttgatttct gactgggtga gcatcgcttt ttgccgctca tattgcccgg
    21901 caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac
    21961 tgaataaacg gtttttggct tcatacaatt tgatggtgcc catcacgcgc tgattttcgc
    22021 cacgcaacgg aattaccagc gtcgacccca gtttgcattg cggatgcaaa gagcaacgat
    22081 aaggtacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt
    22141 aagtcgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca
    22201 gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct
    22261 gagccacttt catgctgttc acttcgttaa acccctgtcg caaaatgcct tccgtcgagg
    22321 ctgccacttt cagcgcagtg gcagaaaaag cagaagtgta tttttcaaac atcgcgcgtt
    22381 tatcgagcaa tatacgcata aacagcgccg cgccgacggt attggtgacc atcattggtg
    22441 cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc gcaaggatga
    22501 tcagcatttg caccatttca gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga
    22561 ctttatcagt gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt
    22621 caacgatggt tgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta
    22681 agccgccggt cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta
    22741 ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc
    22801 ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg
    22861 tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa
    22921 aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc
    22981 acacttccct ttaaaacgcg taacatacat tgcctgcgtt taactttctt tgaactcttg
    23041 cagaaaaatg agaattcgtg agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac
    23101 cctatagatg cacaaaaaac gggcaaaact acctggttcg caaaactgcg tctaaagtta
    23161 aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt acacaattgg tgaagtggcg
    23221 ttgctttgtg atattaaccc tgtcacgtta cgcgcgtggc agaggcgtta cgggttgctg
    23281 aaaccgcaac ggacagacgg cggtcatcga ctgttcaacg atgccgatat tgaccgtatc
    23341 cgcgagatca aacgctggat cgacaacggc gtgcaggtca gcaaagttaa aatgctgctc
    23401 agtaatgaaa atgttgatgt gcagaacggc tggcgcgatc agcaagaaac attactgacc
    23461 tacctgcaaa gcggcaatct gcatagcctg cgaacgtgga tcaaagagcg cggtcaggat
    23521 taccccgccc agacactcac cacacatctg tttattcctc tgcgccgacg acttcaatgc
    23581 caacaaccga ctctccaggc gctgctggcg atcctcgacg gcgtactgat caactacatc
    23641 gccatttgtc tggcttcggc acgtaaaaaa cagggtaaag atgcgctggt ggttggctgg
    23701 aatattcagg ataccacccg tctgtggctg gagggctgga ttgccagtca acaaggatgg
    23761 cgcattgatg tcctcgccca ctcgctcaat caactacgcc ctgaactatt cgaaggccgt
    23821 acattgctgg tgtggtgcgg tgaaaatcga acctccgccc aacagcagca actcaccagt
    23881 tggcaagaac aaggccatga tattttacca ctcggcattt aatgattcgt taacaaatgc
    23941 gctttactgt acaatccttt cgttaacata aggagtgcat tatgcgcata gctaaaattg
    24001 gggtcatcgc cctgttcctg tttatggcgt tcggcggaat tggtggcgtc atgctcgcag
    24061 gttatacctt tattttgcgt gctggctaag cgcctgcacc agcctttcaa acaggcggtc
    24121 tgcgatgatc gccgccagtg ccaccagtaa cgccccctgg atcacatacg cggtattaaa
    24181 tccgctaagc ccgatgatga tgggcgtacc cagcgtgctg gcccctaccg ttgaggcgat
    24241 cgtcgccgta ccaatgttga taatcaccga agttcgcacg cccgccagaa tcaccggagc
    24301 cgccagcggt agctcgacct tacgcagtcg ctgaccacga ctcattccca tacctttcgc
    24361 aacttctgtc acgctggcat cgatcgctcc cagcccggca agtgtcgcct gcaggacggg
    24421 cagcacaccg taaaggatca aggcgataat cgctggttgc agaccaaagc cgatcaccgg
    24481 aacggcaatc gccagcactg cgacaggcgg aaaagtctgt ccaacggcgg caatagtttc
    24541 caccagtggg cgaaattccg cgccccacgg gcgagtgaca gcaattccgg caccagtgcc
    24601 aatgatcacc gcaaacaaac tcgaaattcc caccagccag aaatgagcca gtgccagagc
    24661 tgcaaaactt tcttgctgat aaacgggtcg tggcagttgt gggaacaagg cagcaaacag
    24721 cggctggctg taaggcagcc agaaaatcag tgccacaaac aaagcaatga gccagaacag
    24781 cggatcgcgc aacatcttca tacgcttacg cctccaccag cagatcctga aaatgcagcg
    24841 taccgcaagg ctggccctgc gtgttcacca ccggcagcac ctcgcaaccc cgcgcgacaa
    24901 acagggagag cgcatcgcgt agcgtcatct cttctaccag tgcctcacct tctgcccgtt
    24961 cttcgcgacg cacgtaatcc gccacactac gtaacgaaag cagacgcaca cccagttcgc
    25021 tacgtccaaa aaactggcgg acaaaatcat tcgccggacg agtcagcatc gtcagcggat
    25081 tgccctgctg caccacttca ccgtgatcca tcaataccag atgttcagcc agccgtagcg
    25141 cctcatcaat atcatgagtg accagcacaa tagtgcgccc cagcaaacgg tgaatgcgcg
    25201 tcatctcttg ttgcaacgcg ccgcgcgtta ccgggtccag tgcgccaaaa ggttcatcca
    25261 tcagtaagac ttgcggatcg gcagccagtg cgcgcgccac tcccacacgt tgctgctgac
    25321 caccggaaag ctgatgcgga taacgctcac gcaaatttga ctccagcccc agtagcgtca
    25381 ttaattcgtc gatacgatcg tcaatccgtg cccgcgacca tttttgtaat tgcggcacgg
    25441 tagcgatgtt ttgcgccacg ctccagtggg ggaacaggcc aatagattga atggcatagc
    25501 ccatccggcg gcgcaactcc agtactggca gcgagcgaat ttcttctccg gcaaagcgga
    25561 tctctccgct gtcatgttcc accaggcggt taatcatttt caaggtggtg gatttgccgg
    25621 agccagatgt gccaatcagc accgaaaaac tcccttcctg aaaattgaga ttaagatcgt
    25681 taacggcttt ttgtgcgcca aacagtttgc tgacatggct aaattcaatc attacgtttc
    25741 accttcagca gtgcgataag caaatcgaac agggcatcga tcagcaccgc cagaacaatt
    25801 accgggatca cccccagcaa cactaaatca atggcgctgc ttagcagccc ctggaaaacc
    25861 agcgcaccaa aaccgcctgc gccgattaac gccgcaatca ccgccatacc tacagtttgc
    25921 accatcacca cccgcaggct gcgcagaaat accggtaacg ccagtggtaa ctgaacatgc
    25981 aggaatcgct gcgccccgct catgcccatc gctctggcgc tctccagcac atcgcgcggg
    26041 atctggttca aaccgactac cacgccgcgc accagcggca gcaaggcata gagcaccagc
    26101 gcaatcagtg cgggtgtcat tccggttcct gctatgccga gcttccccag ccacggaaag
    26161 gccgtcacca gcgcggcaag cggcgcaatc aacaggccaa agagcgccac cgaaggcacg
    26221 gtctgaatga cattgagcag agaaaaaatt gccccctgcc gcgcagtgga aaagtagcac
    26281 cagatgccca acggcacacc aatcactaac gcaggcagca ccgcaccaaa cagcaacgtc
    26341 agatgttgtg ccagcgcgtc gtcaaacaca tcctgacggt tggcgtattc tttcattagt
    26401 gagagatcgt taagcgtgcc ggagtacagc aaccacagcg gaataatggc aatctgcata
    26461 tgcaacaacc agcgccacag cggatgcgtg gagattcggc ggatggcatc gctacaggcc
    26521 agcaatgcca gcgccgcagc cagccagaaa ccactgccga ggctggtacg cgccagcgca
    26581 ctgccatttt gcgccagttg ggtcgccgcc tttccagctc cccacaccaa caatacgaag
    26641 acgaattgcg ccagaatgag tgcacaaatg ctgccttttt tagcggggac aaaacaggct
    26701 gtcagccagg cgcaaccaac gcccaccagc atccagatcg tttgcggcca cagttgccag
    26761 agatgacgcc cttcacccga gactaaacga ttaggcgcgt agctgataaa cggcagcgct
    26821 gctgcgattg ccgtcagcaa cagcagcagc gccagaacag gattaatacg tagataagtc
    26881 acgggaaatt acttcgccca ccctttttgt ttcaggtagt cggcagccac ttttttggca
    26941 tccagtcctt ccactgcaat gctggcattc agttgctgca atgtttttgc atcgaggctg
    27001 gcgaagactg gctgtagcca ctgtgccatt tgcggatact ccttcaacac cgattcacgc
    27061 accactggtg caggcgcata gataggttgc acgccttgcg gatcgcttaa ggtttgcagc
    27121 cccagcgccg ctaccgggcc gtcagtgccg taagccattg cagcattaac gccagaggtt
    27181 tgctgggcag cggctttgat cgtcaccgcc gtgtcgccac cagccagtga cagcaactga
    27241 tcctgaccga gcttaaagcc ataggctttt tcaaacgcgg gtaacgcatc ggcgcgttcg
    27301 ataaactctg ccgaggctgc cagtttgaag gtgccgccct cttgcagata acgactcagg
    27361 tcggcaagcg aagtgagttt atttttctct gccacatcct gacgcacggc gatagtccag
    27421 gtgttatttg caggcgcggg cgtcagccag attaacttgt tgtgctccga atcgagcttt
    27481 ttgactttct cgtaaccttg ctgcgcgttt ttccacgctg catcgttttc atctttaaag
    27541 aagaaagcgc cattgccggt atattccgga tagatatcca gttcaccgga agtaatcgcc
    27601 ccgcgcacca caggagtcgt tccgagttgc actttattca ccgtcggtac gccgtggctt
    27661 tccagcacct gcaaaatgat attgccgagt agcgcacctt cggtatcgat ttttgaaccg
    27721 actttaacgg gggaagccgc ctgtagcggc aggctcacgg ctgccaacat aaccagtgaa
    27781 cctgcccaga gctttaagag tggcatgatg ctttcctcat tcttttactg ttgttttcag
    27841 cgaattaaga gaaaagcata gttgataatg gcggggttag cttgagcgga aggattcagt
    27901 tgcagaatca gataaatact taagaggcat attcggtccg gcgtttcctg ccggatgcgg
    27961 cgcgagcgcc ttatccggcc tacaaagggc gcaaacgtcg cgcccttact aaagcattac
    28021 agcaactcaa actcgccttt cttaacgcgt gcggaatcag tgccgataaa tacattgaac
    28081 ttgccaggct cggcgtcata tttcatctgt tgattccaga acttcagcgc ctcaatatcg
    28141 atcgggaagc tgacagtctg agtttcgccc ggtttcaggg tgattttctc aaagcctttc
    28201 agctgtttca cagggcgact catggaagcc gtcacatcct gcaagtacat ctgcactacc
    28261 gtggccccct cgcgcttacc ggtgttcgtc acctgcacgc tggcagtcac tttgccgtca
    28321 cgcttcatgg tcggcgcaga aagtttcaca tcagagacgg tgaaagtggt gtagctcagc
    28381 ccatagccga acggatacaa cgccccgtta gcttcatcaa aataacgcga agtgtatttg
    28441 ttcggcttgt cggcattata cgggcgaccg gtattcagat ggctgtagta caccgggatc
    28501 tgcccgacag aacgcgggaa ggacattggc agcttgccgg acgggttgta atcgccaaac
    28561 aatacatcgg caattgcatt accgccttca gtccccgcaa accaggtttc cagaatcgca
    28621 tcagcctgct gatcttcttt caccagcgcc agcggacgcc cgttcatcag caccagcacc
    28681 agcggtttac cggtggcttt cagcgccgca atcaagtcac gttggctttg cggaatagtg
    28741 atatcggtcc ggctggaggc ttcgtgcgcc atcccctgtg cttcaccgac tacagccacc
    28801 accacatcag attgtttcgc cgtctgcacc gcttcatcaa tcatctcttg cggcgaacgc
    28861 ggatcgactt tgaccgcttc ttcatactga ttcaggaaat cgataatgcc tttgtcactg
    28921 gtaacgttcg cccctttggc atacagcact ttaccgtttt caccgacgga atttttaatc
    28981 ccggtcagta cggtcacgga ttgatcggca acaccggctg cggaccagct gcccatcacg
    29041 tcacgtttac tgtccgccag tggcccaacc accgcaatgg tggccgattt tttcagcggt
    29101 aacgtttcga gacggttttt cagcaacacc aggctttcgc gcgccacttc acgcgcttct
    29161 ttacggtgca ggcggctttc ggcattggta tccaccgggt cagactcttt cggccccaaa
    29221 tggctgtatg ggtcgttaaa caatcccata tcatatttaa cgttcagtac atggcgggca
    29281 gcatcgtcca gctcttccat cgtcactttg ccggagttga tcaacccagg cagatacttc
    29341 gagtaatact cgtcgctcat actcatgttg attccggatt tcagcgccac gcgcaccgca
    29401 tcttccgggt ctgccgccgt gccatgttta atcagctctt tgattgcacc gtgatcggaa
    29461 acggtgatgc ctttaaagcc ccactggtcg cgcagaacat ctttcagcag ccaggaatcg
    29521 gaggtggctg gcgtgccgtt cagcgagttc agcgccacca tcaccgcgcc gctgcctgcg
    29581 tccagccccg ctttgtacgg cggcatataa tcattaaaca ggcgctgcgg actcatatcg
    29641 acggtgttgt actctttacc gccttctacc gcgccgtatg cggcaaagtg tttgacgctg
    29701 gtcatcaccg agtagcgatc tgccgggctt ttaccctgca tcgcttccac catggtttta
    29761 cccattgttg aggtgagata cgtatcttcg ccaaaacctt cggaagcacg tccccagcgc
    29821 ggatcgcgcg agacatcgac catcggtgcc caggtcatat tcaggccatc atctgccgct
    29881 tcataagcag agacacgtcc gaccgttttc actgcatcga ggttaaaaga cgaggccaga
    29941 ccgaggctaa tcgggaacac cgtgcgctga ccgtgcagca cgtcgtaagc aaagaaaaga
    30001 ggaattttca ggcggcttaa ttccatcacc tgatcctgca tggcgcggat atcctgacgg
    30061 gttacggtgt tgaaaatcgc cccaacctga ccgtctttga tcatctcgcg gatcgcctct
    30121 ttcgggttat ccgggccgac gctgattaag cgcagctgac caattttctc atcaactgtc
    30181 attttcttaa gcagttcggt gacgaacgca tcccgcgctt cgggcgttaa tggatggttg
    30241 ccgaataaat catccgccag tgctggctgc agggccagac tcaccgcgat tcctactgaa
    30301 catagccatt tcatatggat ttttcttcct ctgttgcccg acgaggcagc gaaaatggtg
    30361 caaaaaccgt agtttgccat aagcatgatg gagagagaaa aagaatgctc agtttattgt
    30421 ctgaattttc aaaatattca ctcgctgaat tgtcatacaa ggcgctattc tagtttgtga
    30481 tattttttcg ccaccacaag gagtggaaaa tgtcttccat gacaacaact gataataaag
    30541 cctttttgaa tgaacttgcc cgtctggtcg gtcattcaca cctgctcacc gatcccgcaa
    30601 aaacggcccg ctatcgcaag ggcttccgtt ctggtcaggg cgacgcgctg gctgtcgttt
    30661 tccctggctc actactggaa ttgtggcggg tgctgaaagc ctgtgtcacc gccgacaaaa
    30721 ttattctgat gcaggctgcc aatacaggcc tgaccgaagg atcgacgcca aacggtaacg
    30781 actatgatcg cgatatcgtg atcatcagca ccctgcgtct cgacaagctg cacgttcttg
    30841 gcaagggcga acaggtgctg gcctatccgg gcaccacgct ctattcgctg gaaaaagccc
    30901 tcaaaccgct gggacgcgaa ccgcactcgg tgattggatc gtcgtgtata ggcgcatcgg
    30961 ttatcggcgg tatttgtaac aactccggcg gctcgctggt gcaacgtggc ccggcgtata
    31021 ccgaaatgtc gttattcgcg cgtataaatg aagacggcaa actgacgctg gtgaaccatc
    31081 tggggattga tctgggcgaa acgccggagc agatccttag caagctggat gatgatcgca
    31141 tcaaagatga cgatgtgcgt cacgatggtc gtcacgccca cgattatgac tatgtccacc
    31201 gcgttcgtga tattgaagcc gacacgcccg cacgttataa cgccgatccg gatcggttat
    31261 ttgaatcttc tggttgcgcc gggaagctgg ccgtctttgc ggtacgtcta gataccttcg
    31321 aagcggaaaa aaatcagcag gtgttttata tcggcaccaa ccagccggaa gtgctgaccg
    31381 aaatccgccg tcatattctg gctaacttcg aaaatctgcc ggttgccggg gaatatatgc
    31441 accgggatat ctacgatatt gcggaaaaat acggcaaaga caccttcctg atgattgata
    31501 agttaggcac cgacaagatg ccgttcttct ttaatctcaa gggacgcacc gatgcgatgc
    31561 tggagaaagt gaaattcttc cgtccgcatt ttactgaccg tgcgatgcaa aaattcggtc
    31621 acctgttccc cagccattta ccgccgcgca tgaaaaactg gcgcgataaa tacgagcatc
    31681 atctgctgtt aaaaatggcg ggcgatggcg tgggcgaagc caaatcgtgg ttggtggatt
    31741 atttcaaaca ggccgaaggc gatttctttg tctgtacgcc ggaggaaggc agcaaagcgt
    31801 ttttacaccg tttcgccgct gcgggcgcag caattcgtta tcaggcggtg cattccgatg
    31861 aagtcgaaga cattctggcg ttggatatcg ctctgcggcg taacgacacc gagtggtatg
    31921 agcatttacc gccggagatc gacagccagc tggtgcacaa gctctattac ggccatttta
    31981 tgtgctatgt cttccatcag gattacatag tgaaaaaagg cgtggatgtg catgcgttaa
    32041 aagaacagat gctggaactg ctacagcagc gcggcgcgca ataccctgcc gagcataacg
    32101 tcggtcattt gtataaagca ccggagacgt tgcagaagtt ctatcgcgag aacgatccga
    32161 ccaacagcat gaatccgggg atcggtaaaa ccagtaaacg gaaaaactgg caggaagtgg
    32221 agtaaaaatt acggatggca gagtatcgcc atccgaattc acttaatcgt tctgtgccgt
    32281 ctgccccgcc gccgccattt gggcggcttt ttgtttttta tagctcaacg ctgctgccgg
    32341 cacaggcatc actttaccgg tttcaatcca ggtacgcagg cggctggcat cggcaaaatg
    32401 ggtatatttg ccaaacgcgt ccatcactac cagcgccacc ggtttattat tgataaccgt
    32461 acgcatcacc agacaatggc ccgccgcatt ggtaaagccg gttttggtta actgaatatt
    32521 ccagttatcg cgatacacca gatgattagt attgcggaac ggcagcgtat acgtcgggtt
    32581 agagaaggtt gccatatctt cccgggtagt acttaactgc ccgatcaacg gatattgttt
    32641 gctggcaatg agcagtttgg ttaagtcacg ggcagttgaa acgttatgca ccgacaatcc
    32701 ggtaggttca acaaagcgcg tgttgttcat tccgagcgat ttcgctttcg cattcattgc
    32761 cttaataaag gctttgtaac caccgggata atggtgcgca aggcttgccg ccgcgcggtt
    32821 ttctgaagac atcagcgcca gcaacagcat atctttacgg ctgatttcgc tattcagtcg
    32881 tacgcgcgaa tagacccctt tcatctccgg cgtctggctg atatccactt ttagtttttc
    32941 atccagcggc agtcgtgcat ccagcacaac catcgcggtc attaatttgc tgatagacgc
    33001 aattggacgc accagatccg ggtggttcga atagatcact ttgttggtat tcagatcaac
    33061 aatcatcgcg ctaccggagg caatttccgg ttgtgaagcg gcggtagcgg ctgccgtttt
    33121 cgcaacggcc tgcggtgcca aaggcacagc cagcatcagg gccaggctaa ataaagaaac
    33181 tcgaaatttc ggcatgatga gcattcagat agtggttcac gcgcacgggt tgcgcaccgc
    33241 cggagtaagg atttactgag gctagcgacg ccatcataac gagcaaaaag tgcgatcgtc
    33301 aaaggagaat cgtgaggaaa tgctgcattg ctgacattta cgccagcaat gcaatgtcaa
    33361 agaactttct agaacaggcg atacccgtag ccccatagta taacggttag ggcgagcagc
    33421 acttccagta ccagcacgcc aatcgccagc gtcgaactgg agaagctaag gccttcctct
    33481 ttgttgatat tcaggaagct cggaatacca aggtaaagca ggtagccggt gtaaaacagc
    33541 gccaccgtgc cgaccagcgc acacaaccag accagcggat aaagcgccac cagaccgctt
    33601 aaaaacagcg gagttgcaac gtagcccgcg aagaccatgc agtgcgcaag tgacggacgc
    33661 tgcgggtaat tgcgcgccat ccaccagatg acccgcccca tcaccgcgac cccagccagc
    33721 ataacgccgt aaaacaagac agccagcgcc agtccggtaa accaggataa cttcaggata
    33781 gtgccatcgc caaaattcca gccaatctgt gtagtgccaa tgaaggcgca aatcaccgga
    33841 atcgccgcca tcagcaaaac gtggtgggtg taatggtgag aaatcgtttc gttttcgcga
    33901 ttaatcacct gcatttcacg atcgggatgg gaaaacagtc cccagacatg gctcataccg
    33961 cccccttgtt gtgagttcat gaacctgaca gttcaagtat aagtcagctt gtgattattt
    34021 tttgttcgcc catgtaattt tcactgtctg atgaacgtcc tttttcagcg tgaatgattc
    34081 acagggtgta tgcttactgg caaccaaagg gagacagact ggcctatgga tctcaataca
    34141 cttatctcac aatatggtta tgccgcgctg gtgatcggta gcctggcgga aggtgaaacc
    34201 gtgactttgc tgggaggcgt tgcggcacat caggggctat taaagttccc gctggtggta
    34261 ctttctgtgg cgtttggcgg catgattggc gaccaggtgc tttatctgtg cgggcggcgg
    34321 tttggcggca agctgttacg ccgtttctcg aaacatcagg ataaaattga gcgggcgcag
    34381 aaacttatcc aacgccatcc gtatctgttt gtcattggta cgcgctttat gtatggcttt
    34441 cgggtgattg gcccgacgct gattggtgcc agccagctgc cgccgaaaat ctttctgccg
    34501 ctgaatattc tcggcgcatt tgcctgggcg ttgattttta ccactattgg ttacgctggt
    34561 ggtcaggtga ttgcgccgtg gttgcacaat ctcgaccagc atttgaagca ctgggtctgg
    34621 ttgattctgg tcgtggttct ggtggtgggc gtgcgctggt ggctgaaacg gcgcgggaag
    34681 aaaaagccgg atcatcaggc gtaaaaccat tgccctgggt aaggcgttcg cgccgcatcc
    34741 gacatccggt gcaccatgcc tgatgcgacg cttgtgcgtc ttatcatgcc taccaaaatt
    34801 ttacccattg aaataaccac gcacctcact gcgctattct gggttgaact gcggattcgc
    34861 caacataaag ccgccatcca ctatcaacga ctgcccggtg gtgtaatttg cgccctctga
    34921 gcacagccac gctaccaggc tggcaatctc atgcgttgcg ccaaaacgcc gcagaggaat
    34981 cgaaggctcc gcgtcgggct tcacgtcgct gtcatccatg ccattcattg gcgtggcgat
    35041 cgccccaggc gcgactgcgt tcaccaaaat cttatgcctg accagctcca gcgccatcgt
    35101 tttggttaac ccgccgagcg catgtttagc ggctgtgtaa gcgctggcat ccggcagcgg
    35161 cgtatgttca tgtaccgacg taatgttgat gatgcgaccg ccctgccctt gtttcaccat
    35221 ctggcgggcc gcaatttgtg agcataaaaa tgcaccatcg acatcaacgg taaaaatctt
    35281 gcgccactca tcgaaagcca tatcaagaaa cggcgctttg gtcattgcgc ccgcattatt
    35341 caccagcaca tcaatacgcc ccagccgttg aatgagtttc tccagcgcct gcgcaccttc
    35401 tggcagttta ccgagatcca gctgcacgat ctccgcacgt acgccgtggc taactacctc
    35461 acgcgcggta tcttttgccc cttcttcatc tgagtgccag gtaatgccaa tatcaaaccc
    35521 ctgctgcgcc agtaataacg cgcactcttt gccgatcccc gaatcggagg cggtaataat
    35581 cgcaacctgt gccatcgagt tctccactta acgctgaata aacgttaagt atagaaggcg
    35641 catatcatca gcgtttgtaa cccccgccca acgcaccagt gagttgaatg gaagcatcca
    35701 gccactgccc ttgcaataac aggccattgg cccgctcacg cagcgcgggg attctggctt
    35761 cgctgacgcg ggaaccagca atgatgcccg cgttaaagcg cgcctgcgca agacctacca
    35821 cacgcaaggc atcgcgctca atttgcgcct gatgctggtt tttctccgca agtgtctgaa
    35881 cctgactggc tgcccgcgcc acgtcattca ccgcttcaac caccgctttg ttgtagctgg
    35941 cgatagacaa gttgcttccg gcttttgcga tatcgagatt ggcgttaaga cgaccactat
    36001 cgaaaatggg tagcgtcagg cctgccgtaa cgcccatttg ctgcgcggaa tgacggaaca
    36061 gatcgcttaa gtgcaacgca tcctgttgca ggaaggccat caggttgatg tcaggataaa
    36121 atgccgcttt tgccgcatca atggtgctta gcgatgactc aacgtaccag tgcgccgcct
    36181 gcaaatctgc ccgccgggcc agtaaggagt accccagttc atcaggaagc tggcttgcca
    36241 ctttcggcaa cgcgaccggt ttaagcttca atgactttgt ctggttattt gtaagtgcgc
    36301 ttaaccgtgc ctcaataatt ttcattttcc ccgcgacatc gttgagctgc tgccgggttt
    36361 tgctggcatt aatatcggtt tccacaccgt caactgaaga agtaatcccg ttctgatata
    36421 gctggcgatc ggtcgcgata atggtgttct gctctttttc tatttgctgc aagaccgtgt
    36481 ttaacgccgc ctgggtttgc cactcccagt acaggcgggc tacgctgcca gccagcaatt
    36541 ggcgggtttg ctcgcgttcc gccgcccgtg ctttaaccgt acccaggcgg gcagtaacct
    36601 ccgcccgatt ctttccccag atatcgagat gccagcccgc cgttaagcca aaagttccgt
    36661 tggtgtacca cggaccggtc gtacctgcgg ccggatcgtt cagagcaaac ggccccatta
    36721 agccttctgc cgacattttt tgccgctcca tatccgcaga aaagtcgatc tgcggaccat
    36781 cctgagtagc aattgccttc gcctgggctt cagctagctg aatgcgctgt tcagccacct
    36841 gcatatccgg tgcgttctgt agtgcattgt taattaagga agtgagttga ttatcgtgat
    36901 actccagcca ccattggctg tctggccaac catttttcag cgccgtgggt aatgcggtgt
    36961 caacttgtgc agcgggcgtt tgctggctta acgcctggcg ggtttcatgc ataggcgcac
    37021 acccggccag catcagtaac agcggaaaac aggcgatggc gggataaaag gaatcacgat
    37081 tcatggggga ataatcaggt aagaaaaggt gcgcggagat taccgtgtgt tgcgatatat
    37141 tttttagttt cgcgtggcaa tacatcagta gcaataaaac gacatatcca gaaaaatata
    37201 cactaagtga atgatatctt ccgatttatc ttaatcgttt atggataacg gcaaagggct
    37261 tcgttttttc ctatacttat tcagcactca caaataaagg aacgccaatg aaaattatac
    37321 tctgggctgt attgattatt ttcctgattg ggctactggt ggtgactggc gtatttaaga
    37381 tgatatttta aaaatcagat atggcatata agttgtcagg tcaggaacta gatattttgt
    37441 gtacttgatt tgcaaacagc tccggtgttg gtggcgtcaa aatcgctgaa gcgttccccg
    37501 aaggccgggg cagcccacat ggatgtgggc tgagggcgcg tcttacaagg acgtttcct
//
LOCUS       DAZGCS010000043        36501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGCS010000043 DAZGCS010000000
VERSION     DAZGCS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.043"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1091
                     /locus_tag="ACHK01_002217"
     CDS             <1..1091
                     /locus_tag="ACHK01_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8097163.1"
                     /translation="SGLIYLVETIVVIHKPISDSTLIQTKSNDVSIFYIFRQLSFICL
                     TSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPD
                     NHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDE
                     YNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
                     SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLE
                     GEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRN
                     ISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1272..2762
                     /gene="dgcJ"
                     /locus_tag="ACHK01_002218"
     CDS             1272..2762
                     /gene="dgcJ"
                     /locus_tag="ACHK01_002218"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO8097164.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2805..3308
                     /gene="yeaK"
                     /locus_tag="ACHK01_002219"
     CDS             2805..3308
                     /gene="yeaK"
                     /locus_tag="ACHK01_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO8097165.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3309..3413)
                     /gene="yoaI"
                     /locus_tag="ACHK01_002220"
     CDS             complement(3309..3413)
                     /gene="yoaI"
                     /locus_tag="ACHK01_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO8097166.1"
                     /translation="MNDQMFVETLIISSSFFAIAVVLVLSVLLIERTG"
     gene            3583..4029
                     /gene="yeaL"
                     /locus_tag="ACHK01_002221"
     CDS             3583..4029
                     /gene="yeaL"
                     /locus_tag="ACHK01_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO8097167.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3986..4807)
                     /gene="nimR"
                     /locus_tag="ACHK01_002222"
     CDS             complement(3986..4807)
                     /gene="nimR"
                     /locus_tag="ACHK01_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO8097168.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVIMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWACFFAMSERNIARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4904..6085
                     /gene="nimT"
                     /locus_tag="ACHK01_002223"
     CDS             4904..6085
                     /gene="nimT"
                     /locus_tag="ACHK01_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO8097169.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6140..6487
                     /gene="yeaO"
                     /locus_tag="ACHK01_002224"
     CDS             6140..6487
                     /gene="yeaO"
                     /locus_tag="ACHK01_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO8097170.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6509..6763)
                     /gene="yoaF"
                     /locus_tag="ACHK01_002225"
     CDS             complement(6509..6763)
                     /gene="yoaF"
                     /locus_tag="ACHK01_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO8097171.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            6946..7971
                     /gene="dgcP"
                     /locus_tag="ACHK01_002226"
     CDS             6946..7971
                     /gene="dgcP"
                     /locus_tag="ACHK01_002226"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO8097172.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLDDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8004..8102
                     /gene="yoaK"
                     /locus_tag="ACHK01_002227"
     CDS             8004..8102
                     /gene="yoaK"
                     /locus_tag="ACHK01_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO8097173.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8105..8179
                     /gene="yoaJ"
                     /locus_tag="ACHK01_002228"
     CDS             8105..8179
                     /gene="yoaJ"
                     /locus_tag="ACHK01_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO8097174.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8238..8486)
                     /gene="yeaQ"
                     /locus_tag="ACHK01_002229"
     CDS             complement(8238..8486)
                     /gene="yeaQ"
                     /locus_tag="ACHK01_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8097175.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8714..9922)
                     /locus_tag="ACHK01_002230"
     CDS             complement(8714..9922)
                     /locus_tag="ACHK01_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8097176.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            9930..10361
                     /gene="tnpA"
                     /locus_tag="ACHK01_002231"
     CDS             9930..10361
                     /gene="tnpA"
                     /locus_tag="ACHK01_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO8097177.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(10377..10565)
                     /locus_tag="ACHK01_002232"
     CDS             complement(10377..10565)
                     /locus_tag="ACHK01_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO8097178.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10569..10928)
                     /gene="yeaR"
                     /locus_tag="ACHK01_002233"
     CDS             complement(10569..10928)
                     /gene="yeaR"
                     /locus_tag="ACHK01_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO8097179.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11099..11737)
                     /gene="leuE"
                     /locus_tag="ACHK01_002234"
     CDS             complement(11099..11737)
                     /gene="leuE"
                     /locus_tag="ACHK01_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO8097180.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(11864..12787)
                     /gene="dmlR"
                     /locus_tag="ACHK01_002235"
     CDS             complement(11864..12787)
                     /gene="dmlR"
                     /locus_tag="ACHK01_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO8097181.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYQNFSLEHA"
     gene            12890..13975
                     /gene="dmlA"
                     /locus_tag="ACHK01_002236"
     CDS             12890..13975
                     /gene="dmlA"
                     /locus_tag="ACHK01_002236"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO8097182.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14226..15836
                     /gene="yeaV"
                     /locus_tag="ACHK01_002237"
     CDS             14226..15836
                     /gene="yeaV"
                     /locus_tag="ACHK01_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO8097183.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFLTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            15868..16992
                     /gene="yeaW"
                     /locus_tag="ACHK01_002238"
     CDS             15868..16992
                     /gene="yeaW"
                     /locus_tag="ACHK01_002238"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO8097184.1"
                     /translation="MSNLSPDFVLPENYCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17048..18013
                     /gene="yeaX"
                     /locus_tag="ACHK01_002239"
     CDS             17048..18013
                     /gene="yeaX"
                     /locus_tag="ACHK01_002239"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO8097185.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18067..19182)
                     /gene="rnd"
                     /locus_tag="ACHK01_002240"
     CDS             complement(18067..19182)
                     /gene="rnd"
                     /locus_tag="ACHK01_002240"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO8097186.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19264..20949)
                     /gene="fadD"
                     /locus_tag="ACHK01_002241"
     CDS             complement(19264..20949)
                     /gene="fadD"
                     /locus_tag="ACHK01_002241"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO8097187.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21154..21735)
                     /gene="yeaY"
                     /locus_tag="ACHK01_002242"
     CDS             complement(21154..21735)
                     /gene="yeaY"
                     /locus_tag="ACHK01_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO8097188.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(21775..22470)
                     /gene="tsaB"
                     /locus_tag="ACHK01_002243"
     CDS             complement(21775..22470)
                     /gene="tsaB"
                     /locus_tag="ACHK01_002243"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO8097189.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22528..24438)
                     /gene="yoaA"
                     /locus_tag="ACHK01_002244"
     CDS             complement(22528..24438)
                     /gene="yoaA"
                     /locus_tag="ACHK01_002244"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO8097190.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLHSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIAIQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24570..24914
                     /gene="yoaB"
                     /locus_tag="ACHK01_002245"
     CDS             24570..24914
                     /gene="yoaB"
                     /locus_tag="ACHK01_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8097191.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            25336..25635
                     /gene="yoaC"
                     /locus_tag="ACHK01_002246"
     CDS             25336..25635
                     /gene="yoaC"
                     /locus_tag="ACHK01_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO8097192.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(25755..25934)
                     /gene="yoaH"
                     /locus_tag="ACHK01_002247"
     CDS             complement(25755..25934)
                     /gene="yoaH"
                     /locus_tag="ACHK01_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO8097193.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            26008..27369
                     /gene="pabB"
                     /locus_tag="ACHK01_002248"
     CDS             26008..27369
                     /gene="pabB"
                     /locus_tag="ACHK01_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO8097194.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            27373..27951
                     /gene="nudL"
                     /locus_tag="ACHK01_002249"
     CDS             27373..27951
                     /gene="nudL"
                     /locus_tag="ACHK01_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO8097195.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            28135..29499
                     /gene="sdaA"
                     /locus_tag="ACHK01_002250"
     CDS             28135..29499
                     /gene="sdaA"
                     /locus_tag="ACHK01_002250"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8097196.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPLKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            29630..31228
                     /gene="pdeD"
                     /locus_tag="ACHK01_002251"
     CDS             29630..31228
                     /gene="pdeD"
                     /locus_tag="ACHK01_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8097197.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(31232..32788)
                     /gene="yoaE"
                     /locus_tag="ACHK01_002252"
     CDS             complement(31232..32788)
                     /gene="yoaE"
                     /locus_tag="ACHK01_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO8097198.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(32776..32934)
                     /gene="yoaL"
                     /locus_tag="ACHK01_002253"
     CDS             complement(32776..32934)
                     /gene="yoaL"
                     /locus_tag="ACHK01_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO8097199.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            33251..34222
                     /gene="manX"
                     /locus_tag="ACHK01_002254"
     CDS             33251..34222
                     /gene="manX"
                     /locus_tag="ACHK01_002254"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO8097200.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            34285..35085
                     /gene="manY"
                     /locus_tag="ACHK01_002255"
     CDS             34285..35085
                     /gene="manY"
                     /locus_tag="ACHK01_002255"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO8097201.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            35098..35949
                     /gene="manZ"
                     /locus_tag="ACHK01_002256"
     CDS             35098..35949
                     /gene="manZ"
                     /locus_tag="ACHK01_002256"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO8097202.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            36004..36462
                     /gene="yobD"
                     /locus_tag="ACHK01_002257"
     CDS             36004..36462
                     /gene="yobD"
                     /locus_tag="ACHK01_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO8097203.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         8908 a         9277 c         8956 g         9360 t
ORIGIN      
        1 taagtggcct tatctattta gttgaaacta ttgttgttat ccataaacct atcagcgaca
       61 gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc cgccaactca
      121 gttttatttg tttaacctcg ctggcgctct tttgttatgg taaagacaac atccttgaca
      181 acaataagaa aaaaactggc attctgttgc tggcgctgat tcctttttta gtttttcccc
      241 ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc gtcgattact
      301 gtccggacaa ccatactgcg acctggggaa tcaactatac aaaaatattg gtttgtctgt
      361 gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa ctatggccgt
      421 taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg actctggatg
      481 agtataatta caccatctgg tatatcagtc gcgggattga agtttccagt aaactgtttg
      541 ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc aaactggcag
      601 ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc gtagagtcat
      661 tattgtcgcg acctgttgtt aaggactttt gtgtcatgct ggttgatatt aatcagttca
      721 aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt tcaattgtcg
      781 atattatcca gcaaagcatc cgccccgatg atattttagc gcgactggag ggtgaggtgt
      841 ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg gaacgtatgc
      901 gtaaaaatgt cgaactcctg accggcttta gtaacagata tgatgttcct gaacaaatga
      961 ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg cttgtcatga
     1021 cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa gtgattattc
     1081 atcatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt tgcatacaat
     1141 ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt ataattagcg
     1201 cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta accgaaggag
     1261 tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc gcaagtgtca
     1321 ttgtgctgac atcttccttc cttatttttg aacttgtcgc cagcgacaga gcaatgagcg
     1381 cctatctgcg ctatattgtg cagaaagcag actcctcctt tctttatgat aagtatcaga
     1441 atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc tgagcagtcg gaagtgtcgc
     1501 cagaaaagcg gcgcgccatc tgcgaggctt ttgagtctgc caataatacc catggcttaa
     1561 acctgactgc ccataaatac ccgggcttac gcggcacact acaaaccgca tccactgact
     1621 gcgacacaat tgtggaagct gcagcgctat tacccgcttt tgatcaggca gtggaaggca
     1681 accgccacca ggatgattac ggttcaggtc ttgggatggc cgaagaaaaa tttcactatt
     1741 atctcgatct caatgaccgc tatgtctatt tttatgagcc ggttaatgtt gaatactttg
     1801 cgatgaataa ctggtccttc ctgcagtcag gaagtattgg catcgatcgc aaagatattg
     1861 aaaaggtatt taccgggcgt accgtattgt cgagcattta ccaggatcag cgtactaaac
     1921 agaacgtgat gagtttgctg acgccggtat atgtcgcagg gcagctaaaa gggattgtgc
     1981 tgctggatat taacaaaaac aatctgcgga atatctttta tactcatgac cgccctctcc
     2041 tctggcgttt tctcaatgtc acgctaaccg ataccgattc ggggcgcgac attatcatca
     2101 accagagcga agataatctg ttccagtatg tcagttacgt ccatgactta ccgggcggca
     2161 ttcgtgtctc gttatccatt gatattcttt actttatcac gtcttcgtgg aaaagcgttc
     2221 tgttctggat tttgacggcg ttaattttgc tgaatatggt ccggatgcac ttccgtttat
     2281 accaaaatgt gtcgcgagaa aatattagtg atgcaatgac tggactgtat aatcgcaaaa
     2341 ttttaacccc tgaactggaa cagcggttgc agaaactggt gcaatccggt tcttcggtga
     2401 tgtttattgc tattgacatg gacaagttaa agcaaataaa tgacaccctc ggtcatcagg
     2461 agggggattt agcgattacg ttattagctc aggcgattaa acagtcgatt cgtaaaagtg
     2521 attatgccat ccgactcggt ggcgatgaat tctgcatcat tcttgtcgat tcgacgccgc
     2581 aaattgcagc acaactgcct gaacgtatcg aaaaacgtct gcaacatatc gcgccgcaga
     2641 aagagatcgg cttctcttcc ggtatttatg cgatgaaaga aaacgatacg ttacatgatg
     2701 cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac agccgttcat
     2761 gataaccttc tgtggttgtt tgcttgtaat ctcaggagca tgaaatgact gaaatggcta
     2821 aaggaagcgt gacgcatcaa cggttaatcg ctttattatc acaagaaggt gctaacttcc
     2881 gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt cgtggcaccg
     2941 ctttaggcca gggagctaag gcactggtat gtaaggtcaa aggcaatggc gtaaatcaac
     3001 atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactc gccagccata
     3061 taggcggatt acgcgcttcg ttggccagcc cggcggaagt cgatgaactc acaggctgtg
     3121 tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt gccgaccctc
     3181 tactcttcga acgatttgat gaaatcgcct tcaatgcagg catgctggat aaatccgtta
     3241 ttttgaaaac cgcggattat ttgcgcattg cgcaaccaga actggtcaat ttccgccgca
     3301 ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac aaccgcaatg
     3361 gcaaaaaacg atgacgagat aatcagtgtc tcgacgaaca tttgatcgtt catagtatgc
     3421 ccccgggaga tagctgtttt cacgttattg ttagcgtgca ccaatggcag tttgatgaca
     3481 gttcgcctga ttttttatgc aaaaaacgta aagattttct acttccttcc tgcagcaagc
     3541 gtaaagtaag caggcttatt attttttggc aaggaaacca cgatgtttga tgtcactttg
     3601 ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc ataacaccac tgtcgccgtt
     3661 tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa gcaccttttt cccgtggatt
     3721 gaaaaacagg ggctaagtat cgggataatc attctgacga ttggcgttat ggcccccatc
     3781 gccagcggga cgctaccgcc ctcaaccctg attcactcgt ttctgaactg gaaatcgctg
     3841 gtggcaattg cagtaggcgt tattgtctcc tggctgggtg ggcgcggcgt gacgttaatg
     3901 ggcagccagc cgcaactggt tgctgggctg ctggtgggca ctgttttggg cgtggcgctg
     3961 tttcgcggcg taccggttgg cccgcttatt gccgcgggtc tggtttcgct gattgtgggg
     4021 aaacagtagt taatcccgca atatatcggc ctggcgtttg ccccaggcct ttcttaaaca
     4081 tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg agcaactttc tgtacagtat
     4141 cgcctttcac taatccctga agcgccataa tcagctgtaa ctgctgtcgc cactggcgaa
     4201 aactcaaccc cgtctctttt actatcagtc gggcaatatt gcgttcactc atcgcaaaaa
     4261 agcaagccca ttgccccaat gacccccact cgacgggctc tttcgccatc atctccacca
     4321 tcgcgcggat tttaggatga gaagaaacgg gtaggtgcag ctgttgttgt ggctgctgag
     4381 ggagttcatc aaacaggacc tgaattaatc tgcgggtcat cggctcagca cgctgagtcg
     4441 tcgtcctgtt tgccagagtt aaaatcaatt cccggcataa gggcgatatt ttcaatgtgc
     4501 aacaggttgt cggcattatc actgcgctgg gttcgataaa cagaaaacag agttcagcgt
     4561 tagctgtcac ttgattgcta tgttccaccc ctcccggtat ccaaaccgca tattgcggcg
     4621 gcaccatcca caaagcattt tccaccgtac aggtaattgc accatgtagc gccagtatca
     4681 gctgtccttt gcggtgctga tgtacaggac tggttaactc atccgttccg gcgtgaatac
     4741 aaaatgcgac cgcggcttca tggtgtcggt cgggttcata gccattgaga ttcaacctgt
     4801 gcatcatttt gtccgaattt agcgataatt tgtcatttta gcttgattca acataacaat
     4861 aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc
     4921 attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt
     4981 acgcgtcacc tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct
     5041 gacgacagcg caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc
     5101 acctttggct gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt
     5161 acttttgatc tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg
     5221 cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat
     5281 taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg
     5341 agctgcagcg gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg
     5401 gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc
     5461 acagtggcga agccaacaac atgcaaattt gagtacctcg cgcgccttac atactcgggg
     5521 tatctggcgt tcgccgcttg cctggcaggt cacattgttt cttgggatca actcactggt
     5581 ctattacgtg attattggct ggcttccggc gatcctcatc agtcgcggct atagcgaagc
     5641 acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat
     5701 ccctcttttc ttacatcatg tgaaagatca gcgtggtatt gcagcgttcg ttgccttgat
     5761 gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg cacgcgatca cctggactct
     5821 gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg
     5881 ggcaagttct gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt
     5941 gctggcagcc tgtgggccgc cgctgatggg taaaatacac gatgctaacg gtgactggtc
     6001 cgtaccactg atgggtgttg ccatactttc actactgatg gcgattttcg gactttgcgc
     6061 cgggagagac aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcatgcgt
     6121 gaaagggaga acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca
     6181 gagcgatggt tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga
     6241 tttagccctt gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt
     6301 tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa tatcttgcag aactggcgca
     6361 acacgagcaa gaaggaaagc ggctggcgga catcgccaaa aaacagcccc tgaccctgct
     6421 ctacgcagca aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag
     6481 cttgtgattt tagtacagca tccggcggtt atttttcacc agccggatgg taccgtcgcc
     6541 acaatgccca ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt
     6601 gcgctgtctg cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat
     6661 ttgccatacc aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag
     6721 ccaggaccag caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat
     6781 ttatccagac ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt
     6841 cactcaaatc atattacgcc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa
     6901 agattaaata atcagcattt acccgccgta tcctggagtt gtttcgtgtc agatcagatt
     6961 atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag
     7021 cttctggaga tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat
     7081 gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg
     7141 gagaatttta ccgtctcgtg ggattactcg ttatgcaaac gcgccattga tgaaaattgc
     7201 tttttcagcg atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc
     7261 atcaccacat ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt
     7321 tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta
     7381 ttcgctggac tgattgcaca atatattcaa aaagaggcgc tggttgaaca gctgcgcgaa
     7441 gccaatgctg cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg
     7501 cgggcgattt ttgaaaatct gacgacgctg ttttctctcg cccggcatct taaccataag
     7561 ataatgatcg cgtttatcga tctggataac ttcaaattaa tcaatgaccg ttttggtcat
     7621 aatagtggcg atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat
     7681 ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg ttgtttcact aaacaacgag
     7741 aatgcggata tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac
     7801 ttagatgatg ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct
     7861 gaaacaaccg atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa
     7921 aaacacaaac agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc
     7981 acatcctttt gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttta
     8041 ttacagcggt cgtattttta gcatggtttt ttattggcgg ctatgctgcc ccgggagcat
     8101 aaagatgaaa aaaacaacga ttattatgat gggtgtggcg attattgtcg ttctcggcac
     8161 tgagctggga tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc
     8221 tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca
     8281 ccaataacgg caaccacgaa gctgccaaaa ttgaagccat cgactttacc aaagccaaac
     8341 agtgtgctga tccatccgcc gactacggca ccgactatcc ccagcagaat agtcataaag
     8401 aatccaccac catctttacc tggcatgatc cacttcgcca gaataccggc aataagccca
     8461 aaaataatcc atgacagaat gcccattgtt tcctcactta tctgttttgc attagcgggt
     8521 tagtcgctga taaaaagcat agcacaacat cgggagggca agatttgtga cgagcatcac
     8581 ggaggatttt tttgcggatg gcacagaaat tgcgccatca acgatcagtg ataattacca
     8641 accacaaaca tcatgttcgt tttccgtgtc ttgacatcct ccacgccctg aatgacgagg
     8701 atccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc
     8761 ggcctgactg caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac
     8821 gagcgccgtt tacatcggcg ttcgctgtat atccacatac ctggcatctg aattgacttt
     8881 gtgacaggcg attttctttc gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg
     8941 ctggcggaac agcaagcacc tgaccgccac gccagagctg cttgtactca agctggcggc
     9001 gcatttcata ccagccctga tccagtatcg aacggtttaa acctgatttt gcccggacat
     9061 tgcgccccgg ctggctgacc gtacccgctg ctgactttga catgttgctg accttcaaat
     9121 cctcaatgac tatcattgcg tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt
     9181 ccctgcggat atttgcgata cgggaatgca gtcgctgtat tttgcgtttc tgcttctgcc
     9241 agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc agcgtcttct
     9301 ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc gtggcgagtt
     9361 tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga gttgatactt
     9421 cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca gtgacatttt
     9481 tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt ttcggcagaa
     9541 aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat gcatcattct
     9601 gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt ttgtaagccc
     9661 gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta agccattgcg
     9721 tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa gggatgtatt
     9781 tattaccggc ctcatgattc tcattctgaa gcgccagagc acgattgaaa acgaaacgac
     9841 atgcgcctgc gaagcgcctc atttcacact cctgttgatc acctggtctt aactggaatt
     9901 taaatgcctg tagtcgtttc acaggccaaa tgatactttg gtctgtgaaa aaagaaaccg
     9961 atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc tttgtcacca
    10021 gatacagacg tcagattttt gaccatgacg cgacagaaaa actacgcgct tacttttcaa
    10081 atgtatgtgc tgattttgaa gctgaacttg ttgaaatgga tggcgaacca gatcacgtcc
    10141 atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac agcctcaaag
    10201 gcgtatcggg taggttactg cgacgagatc gaccagatat tgcagtcagg tattactaca
    10261 aaggcgtttt gtggagtcct ggctattttg ccagtagctg cggaggtgcg ccaatatccg
    10321 tcatccgcca atacattgaa caacagcaaa cacctggtta ggtggaaaac cgcgccttat
    10381 atccccgccc tgaaggacgg ggttttacgg cgcaccggat aagaacgcac ggtattcacc
    10441 agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg cgtctctgtt
    10501 tcataatcga cagaaacgcc attgctgtta ttggtgacgg tcacggtata cgttgctttg
    10561 cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc catcaggact
    10621 tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc ttcaatgttg
    10681 tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat cacctgatca
    10741 ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg cataacggaa
    10801 aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa tattccggca
    10861 ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt ctgtgggatt
    10921 tgcagcatct ttttccctcc ttcgttgaat gcgctgaaaa cggtattcca gccgtttcag
    10981 agtacgcctg ataatttgca ttttaaatac catttattgg ttatttttta gcatcatatc
    11041 agagaagaat cagggaggat tacaagtggg aagcccgcga caacgcgggc tgaatgtatc
    11101 aggattgcag cgtcgccagt cgggcagcga aacccacgaa catcaaacca atcagtgagt
    11161 tgcccacttt agtcagtttc tttttggtac gtatgtactg cgtgacaaaa gcaccagaaa
    11221 taatcaggaa gctcaaatag cagaaactca ccagttccag cgtcgtcgcc agaataaaga
    11281 atgaaattcc cgtatgtggg gcattaacat cgataaactg tacgaaaaac gacacataga
    11341 acaaaatggc tttcggatta gtcaggctca aaattaacgc gcgtttaaaa atagcaccgt
    11401 attggggctc atcggatttg gcctcgttat ttttaccctt cagggtcgcg tagagaattt
    11461 tactccccag atagagcaaa taaaacgcac caagataacg tataatgttg aataatatcg
    11521 gggtggtctt aattaatgtc gccactccag cccatgccag aaacatcaat accgcatcgc
    11581 caataaatac accgcaggcc gcaagataac cgcctttcat accgctactg acgctatttt
    11641 tgagtacaaa cagggtattt ggccctggca ccaacacaat aaaaatggcc ccaaccagat
    11701 aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta attatctttc
    11761 gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag aaaggagaaa
    11821 atcaggtttt aacccgatat caacccgata attgaataat taatcaggca tgctccagtg
    11881 aaaaattctg gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt
    11941 tcgctgacgt cgccagcctt gaaacataaa cggcccagac gttcgctggc tggtaatatt
    12001 ccggtagcac ttgcactaaa tgaccactgg caatgttttc gctaacatcc caccaggaac
    12061 gcagagcaat cccctgccca tcgagacacc actgatggac aatttctcca tgattagacg
    12121 acagtgggcc ggtgacttta atcgcatggt ggccttcttt attgcgtaat tgccagacac
    12181 caaaaggatg atcgcgctct ttaatgacca agcacggcaa tgcagaaaga tcggttaaat
    12241 gtttcggcgc gccatgttgc gcaataaatt ccggcgacgc gcagagaatg cggtaattgg
    12301 tcgccagttt gcgggcgatt aaattagggg cgatgtcatc gccaatgcga atatcgagat
    12361 cgacaccttc attgaccaaa tcgaccagtc ggtcttccac atcaaaacgt aattccagtt
    12421 gcggatacgc cttcgccagc gctgataacg ccggagccac cacctgtcgt ccaaaaccaa
    12481 agctgctgat aatacgcagc atcccctggg gcacctgacg cacgtcagaa agttcgtcca
    12541 tcatctgacc gacatcctgc aaaatccgct gcgcccattc ataaatccgc tctccttctt
    12601 cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc gtttgctcca
    12661 gcaaggcgat gcgcttgctg acgaacgccg gtgaaacgcc cagttcttcc gccacggcgg
    12721 caaaaccagc ccgacgagcc accagcataa agacgcgcaa atcattcagc agcggtaaat
    12781 tattcatgat tcgtgtttta tgtttcacca gttacgggga ttaattcctt ttcagtcaat
    12841 tataggatgg tgatgttgtc aattttgatg gtcaggaagt gagaacccaa tgatgaaaac
    12901 gatgcgtatt gctgcgatcc cgggagacgg gattggcaaa gaagtccttc ctgaagggat
    12961 tcgcgtgtta caggctgccg ctgagcgctg gggcttcgac ttgagttttg agcaaatgga
    13021 gtgggcgagc tgcgagtatt acagccatca cggtaaaatg atgccggacg actggcatga
    13081 gcaacttagc cgtttcgacg ccatctattt tggtgccgtc ggctggccgg ataccgttcc
    13141 ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg accagtacgt
    13201 caacctgcgc ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg gaaaacagcc
    13261 tggcgacatc gatttttacg tggtcaggga aaacaccgaa ggcgaatatt cctcgctcgg
    13321 cggtagagtg aatgaaggta cagagcatga agtcgtcatt caggaatcgg tatttacgcg
    13381 tcgtggtgtc gatcgcattt tgcgttatgc cttcgaactt gcgcaaagcc gcccacgtaa
    13441 gacgctaact tctgccacta aatcaaacgg tttagccatc agcatgccgt actgggatga
    13501 gcgagtggaa gcaatggccg agaattaccc ggagatccgc tgggacaagc agcatattga
    13561 tattctctgc gcgcgttttg tgatgcagcc ggaacgcttc gatgtggtgg tggcgtccaa
    13621 tttatttggc gatatccttt ccgatcttgg cccggcctgc accggcacca ttggcattgc
    13681 cccatccgcc aacctgaatc cggaacgcac tttcccgtcg ctcttcgagc cagtccacgg
    13741 ttccgcgccg gatatctacg ggaaaaacat tgctaaccct atcgccacga tttgggccgg
    13801 tgcaatgatg ctcgattttc tcggcaatgg cgatgagcgt ttccagcaag cgcataacgg
    13861 tattctggca gcaattgaag aagtgattgc tcacgggccg aaaacacctg atatgaaagg
    13921 caatgccacc acgccacagg ttgccgacgc gatttgcaaa attattttgc gttaaggtca
    13981 aaccagttta tttgaaccgc gtcactgacg cggttttttt attcgttctt tgcagtaaat
    14041 aacctgcgtc atttcacctt ttattgtttc cgtttcgtgt tttatggctt tccgtattct
    14101 taattgttta atttatgtaa catgcaaata atcaatttgt taaacacaac atacaactca
    14161 ttaccctgca aataattaca actggcaaaa gccagagacg ggcttttatg cccaggagat
    14221 gactcatgat gagcaatgtt aagaaaaaag atgtgccgct gataagcatc agcctggtgg
    14281 ccattctttt catcgcagct gcattaagcc ttttcccaca acaatcggcc gacgcggcca
    14341 acgcaatata cacttttgtt acgcgtacgt taggttccgc cgtacaggta ttggttttgc
    14401 tggcaatggg actggtgatt tatttagcca ccagtaaata cggcaatatt cgtcttggcg
    14461 aaggaaaacc ggaatacagc acgctctcct ggctgtttat gtttatttgt gccggtttag
    14521 gttcttctac gctttattgg ggggttgctg aatgggccta ttattatcaa acgcctggat
    14581 taaatatcgc accgcgttca caacaggcac tcgaatttag cgtcccttac tccttcttcc
    14641 actggggcat cagcgcctgg gcaacttata cgctggcctc attaatcatg gcttatcact
    14701 ttcatgtgcg taaaaacaaa ggtctgagtc tttccggcat tattgccgcg attaccggcg
    14761 ttcgcccgca aggcccatgg ggaaaactgg ttgatttgat gttcctgatc gccactgtcg
    14821 gcgcactgac tatttccctt gttgtcaccg cagcaacctt tactcgtgga ctttccgcgc
    14881 tgaccggttt acccgataac ttcaccgtgc aggcatttgt gatcctgctt tccggcggca
    14941 ttttttgcct aagctcgtgg attggtatca acaacggttt gcaacgtctg agcaaaatgg
    15001 ttggctgggg cgcgttcctg ctgccattac tggtgctgat tgtcggccca accgaattta
    15061 ttaccaacag catcatcaat gccatcggtc tgaccacgca aaacttcctg caaatgagct
    15121 tattcaccga tccgcttggc gatggttcat ttacccgcaa ctggaccgtt ttctactggc
    15181 tgtggtggat ctcatacacc cctggcgtag caatgtttct cacccgcgtt tcccgcggtc
    15241 gtaagattaa agaagttatc tggggactga tcctcggcag caccgtcggt tgctggttct
    15301 tcttcggcgt aatggaaagc tatgccattc atcagtttat caatggcgta atcaacgtcc
    15361 cacaggtgct ggaaacactg ggcggcgaaa cagctgtaca gcaagttctg atgtcgttgc
    15421 cagccggtaa attgttcctc gccgcatacc tgggcgtgat gattattttc cttgcctcgc
    15481 atatggatgc ggtggcctac accatggctg cgaccagtac gcgtaatctc caggaaggtg
    15541 acgatcctga ccgtgggctg cgtcttttct ggtgcgtggt gatcactctg atcccgcttt
    15601 ccatcttgtt taccggtgct tcgctggaaa cgatgaaaac caccgtcgtg ctcacagccc
    15661 ttcccttcct cgtcatttta ctggtgaaag tcggcggatt tattcgctgg ctgaaacagg
    15721 attacgccga cattccggct catcaagttg aacattatct cccgcagaca ccggttgaag
    15781 ccctggaaaa aacgccagtg ctccctgcgg gaaccgtatt caaaggcgac aactgagcgc
    15841 catcatccta acgataaagg tatccctatg agcaatctga gccctgactt tgtactaccc
    15901 gaaaattatt gcgctaaccc gcaagaggcg tggaccattc ctgcccgttt ttataccgat
    15961 cagaacgcgt ttgaacacga aaaagagaac gtcttcgcca aaagctggat ttgcgtcgct
    16021 cacagcagcg aactggcgaa tgccaatgat tatgtgacgc gtgagatcat tggcgaaagc
    16081 atcgtgctgg tacgcggtcg tgataaggtt ttgcgcgcgt tctataacgt gtgtccgcac
    16141 cgtggtcatc agttgttgag cggtgaagga aaagcaaaaa atgtgattac ctgcccgtat
    16201 cacgcatggg cattcaaact cgatggcaac ctggcccatg cacgtaactg cgaaaacgtc
    16261 gccaatttcg atagcgacaa agcgcaactg gttccggtgc gtctggaaga atatgccgga
    16321 ttcgtcttca tcaacatgga ccccaacgcc accagcgtag aagatcaatt acccggcctg
    16381 ggcgcgaaag tgctggaagc ctgcccggaa gtccacgatc tgaaactggc ggcccgcttt
    16441 accacccgca cgcctgccaa ctggaagaac attgtcgata actatctcga gtgctatcac
    16501 tgtggtccgg cgcatccagg tttctccgac tccgtacagg ttgatcgtta ctggcacacc
    16561 atgcacggta actggacgct gcaatacggt ttcgccaaac cgtccgaaca gtcgtttaaa
    16621 tttgaagagg gtacggatgc ggcattccac ggtttctggc tgtggccgtg cacgatgctg
    16681 aacgtcaccc cgatcaaagg gatgatgacg gtcatttatg aattcccggt ggattctgaa
    16741 actaccctgc aaaactacga tatttacttc accaatgaag agttaaccga cgagcaaaaa
    16801 tcgctgattg agtggtatcg cgatgtgttc cgtccggaag atttacgtct ggttgaaagc
    16861 gtacagaaag ggctgaaatc gcgtggctat cgtggtcagg ggcgcatcat ggccgacagt
    16921 agcggtagtg gcatttccga acatggtatc gcccatttcc ataatctgct ggcgcaggtg
    16981 tttaaggact aatgacatcg gcggcggtat tttccgccgc tgggctgatt tttgatggag
    17041 tacagcaatg tcagactatc aaatgtttga agtacaggtg agtcaggtgg aaccgcttac
    17101 cgaacaggtg aaacgcttca cgctggtggc aaccgatggc aaaccgctgc ctgcgttcac
    17161 cggaggaagt cacatcattg tgcagatgag cgatggtgat aaccagtaca gcaatgcgta
    17221 ttcactactg agttcgccgc atgacacctc ttgttatcag attgccgttc ggctggagga
    17281 aaactcgcgc ggcggttccc gctttttgca tcagcaggta aaagtgggcg atcggttaac
    17341 gatttcaacg cctaataacc tgtttgcgct aattccctca gccagaaagc atctgtttat
    17401 cgcgggcggt attggtatca cccctttcct gtcgcacatg gcagagctgc aacacagcga
    17461 cgtcgactgg cagctacatt actgctcgcg aaatccagaa agttgcgcat ttcgtgatga
    17521 gctagtccag catccgcagg ctgagaaagt tcacttgcat cactcatcaa ccggaacacg
    17581 actggaatta gcgcgattat tggcggatat cgaacctggc acacacgttt atacctgtgg
    17641 tcccgaggcg ctaaatgaag cggtaagaag tgaagctgcg cgtctggaca tcgtcgccga
    17701 tacgctgcac tttgagcaat ttgctatcga agacaaaacc ggcgatgcct ttaccctggt
    17761 gcttgcccgt tccggaaaag agtttgtggt gccggaagag atgactattt tgcaggttat
    17821 tgaaaataat aaagccgcga aagtggaatg tttatgtcgt gaaggggtat gcggaacctg
    17881 cgaaacagca atactggaag gtgaagctga ccatcgggat cagtatttta gcgatgaaga
    17941 gcgtgccagc cagcaaagta tgttgatctg ttgttcgcgt gcgaagggta aacgcctggt
    18001 gttggatttg tagtttgcca aagccggatg tggcgctgag cgcgcccagt ccggcttcgg
    18061 aagattttac tgcggatatt cctgcaataa attgtgtaat gcttccgcca tcagctcacc
    18121 acgccagccg gaaatcagct ccggcaaatt gttctgcggt ttcagtttcc agtgccagtt
    18181 cagcagttgg ttgatttgcc gacgcgatgc cagcaattcg gcgctgatct tatgcgtttc
    18241 gctcacgtca gtaatcagcg acttaatcgc tttaaacgct ttacgataac caggcatgtc
    18301 catcaggtta agcatcggct gcggtaaggc ctcttccggc aatgtctgcg ctttttccac
    18361 cagcgccagc agcgttttac cgtgaaagcg gatttcgcta ccggataaac ccaggctgtc
    18421 cagttcgcct aaacttcccg gcatataacg cgcgaccgac cacaaatgct cttcacgcac
    18481 gacaaagttc accgccagat cacgctctcg cgccttgcgc agtcgccagt cggctaacag
    18541 ttgcagacag gccagttggc gtgtgcgtaa ctgccaggca ttggtgatat cacgccaggc
    18601 atcttccggc gcaacgactt cctgacgacg catttgcatc aggcggcatt catccagcgc
    18661 cgcaggtagc cagccggagg cctccgtttc taccataagc ttggcggtga tcggtaacag
    18721 ataccagaca tccgccgctg cgtattcaca ctgacgttcg gtcagcggtc tggccagcca
    18781 gtcggtgcgc gattcactct tgtccagcgt aacgccggaa tactcttcca ccatggaagc
    18841 gaaaccccat gacatcgggc gtccgcagaa ggcagcaagg atttgcgtgt caatcaaggg
    18901 ttgtggtaat tcgccaaaga cattgaggaa cacttccaga tcttcactgc ctgcatggag
    18961 aaattttgtg atggacggat cgcgcaggat cgctttcagc ggtgaccagt cggtgatccc
    19021 gagaggatca attagcgcca gatgctcgcc atcgaaaagt tgaatcaacc ccagctgcgg
    19081 gtaataagtg cgcgtacgaa caaattcagt atccagggct atcgccggaa aggcacggac
    19141 ggcttcacac aaagaagcca gcgcatcgtc cgtggtaatc atttggtaat tcaaattgtt
    19201 ttctctttag tgggcgtcaa aaaaaacgcc ggattaaccg gcgtctgacg actgacttaa
    19261 cactcaggct ttattgtcca ctttgccgcg cgcttcgtca cgtaattctc gtcgcaaaat
    19321 ttttccgacg ttagatttcg gtaactcatc acgaaactcc accagcttcg gtactttgta
    19381 gcccgtgagc tgacggcggc aaaaggtcac cagtgactct tcggtaagcg atggatcttt
    19441 tttcactacg aagattttca ccgcttcacc actggagccg gaaggtacgc caacagccgc
    19501 gacttcctgt acgccaggat gctgcatgac gacatcttca atctcgttgg gatagacgtt
    19561 aaaaccggaa accagaatca tgtctttttt acgatcgaca atgcgcagga acccttcttc
    19621 atccatcacc gcgatgtcgc cggtgtgtaa ccagccattt ttgatgatct catctgtagc
    19681 atccggacgc tgccagtaac ccagcatcac ctgcggtcct ttgacacaaa gctcacccgg
    19741 ttgacccggt ggtacttcat tatcatcatc atccaccagt ttggcttccg tcgacggcac
    19801 cggcaaaccg atgctaccac tatgataatc aatatcatat gggttaacgc tgaccagcgg
    19861 cgcacactcg gtaaggccat agccttccag cagatactgt cctgtcagtt tcacccaacg
    19921 ctctgccacc acttgctgca ctggcatccc tccgcctgcg gaaagatgca gactggagaa
    19981 atccagctgc tggaactctt tattgttcag caacgcattg aacaaggtgt taacgcccgt
    20041 gatagcggta aacggatatt tcgctaactc ttttaccaac cctggaatat cgcgcgggtt
    20101 agtgataagc aggttctgcc cacccagttc gataaacagc aggcagttaa tggtcagggc
    20161 aaaaatgtga tacagcggca gcgccgtcac caccagctct ttgcccggat gcaacagcgg
    20221 accataggtc gcgttaacct gttccaggtt cgccagcata ttgcggtgag tcagcatcgc
    20281 gcctttcgcc acaccagtgg tgccgccggt gtattgcaga aaagctaaat cttccggcac
    20341 cagttcgggt ttgacgtact gcatccggta gccgttatgc agtgcgctac gaaatgaaat
    20401 ggcatctggc agatggtatt tcggcaccaa acgcttgatg tatttaacaa cgaaattgac
    20461 taccgtgcct tttgccgtag atagctgatc gcccatacgg gtcagaatta cgtgctgaac
    20521 ggcggtttta tcaaccactt tttccagtgt gtgagcaaag ttagacacga taacaatcgc
    20581 cgatgcgccg ctatcgttaa gctgatgctc aagctcacgc ggggtataca acgggttaac
    20641 gtttacgacg atcatccctg cacgcaaaat gccaaacagc gccaccggat attgcaataa
    20701 attaggcatc atcaacgcaa cgcgatcgcc tttcttcagc cccaaccctt gttgcaaata
    20761 agcggcaaac gcgcgactgc gttcttccag cttgcggaag gtcattacct cccccatatt
    20821 cacaaacgca ggctgatcgg cgtagcgcgc gaccgactgc tcaaacatat ctaccagaga
    20881 ttgataacgg tcagggttga tctccgtcgg aacgtccgcg ggataacggt taagccaaac
    20941 cttcttcaat tcttcacctc taaaatgcgt gttcgtcgtc atcgcaaccc caaatgatat
    21001 acatgccgtt aacataatat taactcatca taccagcttg ataattaccc aacgaaaagg
    21061 ttgcgaagcg cgtcactatt tatttttatc tttaccgtaa gaatgcagaa acagcggacc
    21121 agccgctgtt tctttttctt taaaaacaag caattattca gttacaactg tttgtactct
    21181 cgcgggaccg ggattatacc agccccatcc gccgtagcca tagggccagc cacgaccgcc
    21241 ataaaaccat ggatcaatcg gctgaggcgg cataatcacc tgctgggtta aatgccaacg
    21301 tttgtaaccc gttacttgca tcaccataaa tttatagggc gtattgccga ttttgccgtc
    21361 aaccgcacca gtgattggcc caactaccgt aaccagttgt ccacggaaat ccaccgggtc
    21421 cagaaaaccg ttcacatcgg cataaatgcg accgcgagaa ggttctccca gcgtcggtct
    21481 ggctccgctg tccagcggta cggtagcaat ttccaggcgg gttttccctt gctggttttg
    21541 taccgcaacc actttgccac caaagcgtgc ctcctgacca acgtacagct gcggcgcact
    21601 catcacccga actaaatctt gttgcggcgt gggactgctg cctttaatgg cgtccggcac
    21661 agtgacacaa ccgctcagca ttagcgcaaa cgtacctgcc agtatgcctt tgataacatt
    21721 cttttgaacc gccatgatgc gactcctttt tttctcaggg catactctta agattcattc
    21781 tttgcccgga agtttcttcc atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc
    21841 caccgccacc gttttaccct cggcaaacat ctgacacgca atcggcagca tatcttccgc
    21901 cgctggcagt aaaacttctc cgtcgcgcaa aaccagcccg ctctctttac cgagatccgg
    21961 ccaggcttgc cagcccgtcc ctaccgttac ccattcgccg gaaagctgtt gcattcgttc
    22021 atgaacgagt tcgggtttga gtacggcttc ggtttcttca ccgtgccaga taccgttttc
    22081 atcacgctga tattcggccc agtaaacttc gcccattcgc gcgtcaatgg ctgccagcac
    22141 gcgggttgcg ccgtttttgc gccacgcccc ttgcgccatc gtcattagcg tggagacgcc
    22201 aatcatcggt agctctgcgc caagtgccag cccttgcgca atgccaatac caatgcgcac
    22261 gccggtaaag ctaccggggc cgcgcccgta agccagagcg ttaatatcag tcagggaagt
    22321 tccgctggtg gtcaggatat cctgcaccat cggtaagatt cgttgagtat gttcacgagg
    22381 gcaaagctca aaatgagcgt tgacagtacc gtcgttccac agggcgacag agcaggcctc
    22441 tgtcgcggta tcgatagcca gaattcgcat gggtcttcgt gcttagatca ataaaaaggc
    22501 gcgcatcata ccatactccg taacaaatta cctggaggat ggtatcgcaa ggaaacgaac
    22561 cgcacgggca atgtcacgag tgcgtggcgc gggcggcaga ctggcgagaa acgtcgcgcc
    22621 gtaaggacgc atcaccagcc gattgtcaca aatcaccagc acgccacgat cgtcggcgtc
    22681 gcgaatcagt cgccctaccc cctgtttgag agtaatgacg gcatctggta gttgcacttc
    22741 atcgaacggg tcgccaccgc gcaaacgaca atcttccatg cgcgctttta acagtggatc
    22801 atccggcgag gtaaacggca acttgtcgat aatcaccaat gacaatgtat cgccacgcac
    22861 gtccacccct tcccagaaac tactggttgc cacaagaagc gcgttaccgg cgctgacaaa
    22921 ttgctgcaac agttgccctt tgctggtttc cccctgcaac aaaacgggaa gcgtcatggt
    22981 agcgcggaac tgctcggcca gatcacgcat catggcgtgc gaggtacaaa gcataaaaca
    23041 acgaccgttg ttagcttcga tgatcggtcg cagcattgcc gctaactggc gagcagaacc
    23101 tggctggttg gtttgcggca gattgcgcgg cacacagagt aacgcctggc ggctgtaatc
    23161 aaacgggctt ggcaatagca acgactccgc ctgttctatc ccaagccgcg aggtgaaatg
    23221 atgcagatcg tcgttcaccg acagcgttgc tgaggtgaag atccagctac caggcttttg
    23281 cgccattaac tctttgaatt tatccgccac gctgagcggc gtgagagcca gagtaaaatg
    23341 gcgcgaagtg cattcgtacc agtagctgta gcccggctga ttgatctctt ttagccgctt
    23401 cagccgtgtg cgatacaacg tagcgcgctc aaatgccgca tccagcaagg cggaacgccc
    23461 cagtgacagt ttcgccacgt cataacaaag ttccagggtg tcatcgagca gtaaaaatgc
    23521 ccgctgaatt tgcggattag ctaacagctc acgcaggtta ccgcgataac ctggctcacc
    23581 gagttgcaga cgaaaatcct gcgcactctg ggcaagacga tcagcgcact tttgtaactg
    23641 ctgggtgtct tttaattcgg tgcggtaagc gatggtgatg tcttttgcca ggtcgagcag
    23701 ttgtcgactg gagagtgact gaccaaaata ctggatggca atgtccggta gctggtgggc
    23761 ttcgtcgaag atcatgacgt ccgcttccgg gatcaactcg ccaaatccac tctctttaac
    23821 caccatatcc gccagaaaga gatgatggtt taccaccacc acatcggcgt ccatcgcttt
    23881 tttacgtgct ttgaccacaa agcaatcttt atacatcggg cagtcgctgc caagacagtt
    23941 gtcgttggtg ctggtaacca gcggccacgc ctgtgaatct tccgccacgc tgacgcaggt
    24001 gctgatatca ccatcgactg tttgattaga ccaggagcgc agcaggatca catcgcttaa
    24061 gatttgtacc ggcagatcgc cccccgccag cgcctgctgt tcgagacgtt cgaggcagag
    24121 gtagtttgag cgccctttca gcagcgccac gttgcccgta tatttcaatg cctttgagac
    24181 tgttggcaaa tcgcggctgt ggagctgatc ctgcaacgct tttgagccgg tcgagataat
    24241 gactttcttt ttcgcccgca gcgcaggagc caggtaagcg taggttttgc ccgtaccggt
    24301 tcctgcttcc accaccagcg gctggccttt ttctatcgcc tgggtgacgg ctaccgccat
    24361 ctgtcgctgt ggttctcgcg gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg
    24421 tgcaaaatcg tccgtcacac taccccctgt tgatttgaac agggattatg tcaggatgag
    24481 ggcgctttcg ccagttgaag tggtgacggc aacctcacat tgtggcagtc tttgcagcac
    24541 gaaaatggaa gtataatgag gacaaaatga tgactatcgt tcgtatcgat gctgaagccc
    24601 gctggtctga tgtagtaatc cacaacaaca cgctctacta cactggtgta ccggaaaacc
    24661 tcgacgccga tgcttttgag caaaccgcca acacgctggc gcagattgac gccgtgctgg
    24721 aaaaacaggg cagcaataaa tcgagcattc tggatgccac cattttcctg gctgataaaa
    24781 acgacttcgc ggcgatgaat aaagcgtggg atgcctgggt tgtggcgggt catgcgccgg
    24841 tgcgctgcac ggtgcaagcg gggctgatga acccgaagta taaagttgaa attaagattg
    24901 tggctgcggt gtaagcttta tcgaagcaaa ataagtcaga cgataattta tcgataatac
    24961 tggtcggttt tacataaatc gaccagagat taagatatta ctgttgatgc tcctgctaat
    25021 tcattattta tttttataat ttcaatttta gctataaagt taactatata aataaacata
    25081 accatgccgt tgtgttcgcc ctctgactag caactctggt tttctttctt agctgaataa
    25141 tctggttata gcgtgaggac tgaccgttaa atttctccat cttactggat aacacaccat
    25201 gagctgctac ctttaaaaca cctgaatgtc aacaggttaa ctcgtgccat atcgttataa
    25261 tccctttgat attagatgca aattaaggtc atatatagtc ttatcgataa catggttaat
    25321 tttaaggata agaatatgcc tgctgtaata gataaagccc tggatttcat tggtgccatg
    25381 gatgtatcag cgccaacacc aagttcgatg aatgaaagca cggcgaaggg aatctttaaa
    25441 tatttaaaag aactgggagt acccgcgagt gccgctgata ttacggcgcg agctgaccag
    25501 gaaggctgga accctgggtt cacggaaaaa atggttggat gggcaaaaaa aatggagaca
    25561 ggtgaacgct ctgtgattaa aaatcctgaa tacttttcaa catatatgca ggaagaacta
    25621 aaagcactgg tctgagttaa atttatatca gcataaatgg gtcaggacgc ttttaatcac
    25681 atataaaaag cgtccttttt ctcacaatca aatgatcatc ggcttctata acgaaatcga
    25741 tacggattag tgtgttactc gtcttcatcc tcaaaacgcg ccacgattcg ctcgccggag
    25801 tggttggcac gcagctcttc tgccaccagc gcgattgcct ggccgctgct catcccttgt
    25861 gccatcagtt cctggatccg ctcgacagct ttttgctgct gttcatgggt gagtgaaggt
    25921 aaacctgcaa acattgttaa ctcctgctaa attgttggcg ctaattattt catgctaccc
    25981 ggcacatagc cagtagagtc aggactgatg aagacgttat ctcccgctgt gattacttta
    26041 ccctggcgtc aggacgccgc tgaattttat ttctcccgct taagccacct gccgtgggcg
    26101 atgcttttac actccggcta tgccgatcat ccgtatagcc gctttgatat tgtggtcgcc
    26161 gatccgattt gcactttaac cactttcggt aaagaaaccg ttgttagtga aagcgaaaaa
    26221 cgcacaacga ccactgatga cccgctacag gtgctccagc aggtgctgga tcgcgcagac
    26281 attcgcccaa cgcataacga agatttgcca tttcagggcg gcgcactggg gttgtttggc
    26341 tacgatctgg gccgccgttt tgagtcactg ccagaaattg cggaacaaga tatcgttctg
    26401 ccggatatgg cagtgggtat ctacgattgg gcgctcattg tcgaccacca gcgtcataca
    26461 gtttctttgc tgagtcataa tgatgtcaat gcccgtcggg cctggctgga aagccagcaa
    26521 ttctcgccgc aggaagattt cacgctcact tccgactggc aatccaatat gacccgcgag
    26581 cagtacggcg aaaaatttcg ccaggtacag gaatatctgc acagcggtga ttgctatcag
    26641 gtgaatctcg cccagcgttt tcatgcgacc tattctggcg atgaatggca ggcattcctt
    26701 cagcttaatc aggccaaccg cgcgccattt agcgcttttt tacgtcttga acagggtgca
    26761 attttaagcc tttcgccaga gcggtttatt ctttgtgata atagtgaaat ccagacccgc
    26821 ccgattaaag gcacgctacc acgcctgccc gatcctcagg aagatagcaa acaagcagaa
    26881 aaactggcga actcagcgaa agatcgtgcc gaaaatctga tgattgtcga tttaatgcgt
    26941 aatgatatcg gtcgtgttgc cgtagccggt tcggtaaaag taccagagct cttcgtggtg
    27001 gaacccttcc ctgccgtgca tcatctggtc agcactataa cggcgcgact accagaacag
    27061 ttacacgcca gcgatctgct gcgcgcagct tttcctggtg gctcaataac cggggctccg
    27121 aaagtacggg ctatggaaat tatcgacgaa ctggaaccgc agcgacgtaa tgcctggtgc
    27181 ggcagcattg gctatttgag cttttgcggc aacatggata ccagcattac tatccgcacg
    27241 ctgactgcca ttaacggaca aatatactgc tctgcgggcg gtggaattgt cgccgatagc
    27301 caggaagaag cggaatatca ggaaactttt gataaagtta ataagatatt acgccaactg
    27361 gagaagtaag acgtggaata ccgtagcctg acgcttgatg attttttatc tcgctttcaa
    27421 cttttgcgcc cgcaaattaa ccgggaaacc ctaaatcatc gtcaggctgc cgtgttaatc
    27481 cccatcgtcc gtcgaccgca accggggttg ttgctgactc agcgttcaat tcatctgcgt
    27541 aaacacgctg gacaagtggc attccctgga ggtgcagtcg atgacacgga cgcatcagtt
    27601 atcgccgccg cgctgcgcga agctgaagaa gaggtcgcta taccgccttc cgccgttgaa
    27661 gttatcggcg tgctgccgcc cgtcgatagc gtaactggct atcaggtaac cccagtggtc
    27721 ggcattatcc cgcccgatct gccgtatcgc gccagtgaag atgaagtctc ggcggtgttt
    27781 gaaatgccgc tcgcccaggc attacatctg ggtcgttatc accctttaga tatctaccgc
    27841 cgtggcgatt cacatcgggt atggctgtcc tggtacgaac agtattttgt atggggaatg
    27901 accgcaggca taattcgtga gctggcgctg caaattggtg tgaaaccctg actatactta
    27961 tctttacatc tacaaaacac tacttgagac aatcatcgca atattagtta aatcgcggtt
    28021 tttgattagt ttaattcatg tgaatagtta agccagtcgc cgcgttccct cttacactat
    28081 gcgctgttat tagttcgtta ctggaagtcc agtcaccttg tcaggagtat tatcgtgatt
    28141 agtctattcg acatgtttaa ggtggggatt ggtccctcat cttcccatac cgtagggcct
    28201 atgaaggcgg gtaaacagtt cgtcgatgat ctggtcgaaa aaggcttact ggatagcgtt
    28261 actcgcgttg ccgtggacgt ttatggttca ctgtcgctga cgggtaaagg ccaccacacc
    28321 gatatcgcca ttattatggg tcttgcaggt aacgaacctg ccaccgtgga tatcgacagt
    28381 attcccggtt ttattcgcga cgtagaagag cgcgaacgtc tgctgctggc acagggacgg
    28441 catgaagtgg atttcccgcg cgacaacggg atgcgttttc ataacggcaa cctgccgctg
    28501 catgaaaacg gtatgcaaat ccacgcctat aacggcgatg aagtcgtcta cagcaaaact
    28561 tattattcca tcggcggcgg ttttatcgtc gatgaagaac actttggtca ggatgctgcc
    28621 aacgaagtga gcgtgccgta tccgctcaaa tctgccaccg aactgctcgc gtactgtaat
    28681 gaaaccggct attcgctgtc tggtctcgct atgcagaacg aactggcgct gcacagcaag
    28741 aaagagatcg acgagtattt cgcgcatgtc tggcaaacca tgcaggcatg tatcgatcgc
    28801 gggatgaaca ccgaaggtgt actgccaggc ccgctgcgcg tgccacgtcg tgcgtctgcc
    28861 ctgcgccgga tgttagtttc cagcgataaa ctgtctaacg atccgatgaa tgtcattgac
    28921 tgggtaaaca tgtttgcgct ggcagttaac gaagaaaacg ccgccggtgg tcgtgtggta
    28981 actgcgccaa ccaacggtgc ctgcggtatc gttccggcag tgctggctta ctatgaccac
    29041 tttattgaat cggtcagccc ggacatctat acccgttact ttatggcagc gggcgcgatt
    29101 ggtgcattgt ataaaatgaa cgcctctatt tccggtgcgg aagttggttg ccagggcgaa
    29161 gtgggtgttg cctgttcaat ggctgctgcg ggtcttgcag aactgctggg cggtagcccg
    29221 gaacaggttt gcgtggcggc ggaaattggc atggaacaca accttggttt aacctgcgac
    29281 ccggttgcag ggcaggttca ggtgccgtgc attgagcgta atgccattgc ctctgtgaag
    29341 gcgattaacg ccgcacggat ggctctgcgc cgcaccagtg caccgcgcgt ctcgctggat
    29401 aaggtcatcg aaacgatgta cgaaaccggt aaggacatga acgccaaata ccgcgaaacc
    29461 tcacgcggtg gtctggcaat caaagtccag tgtgactaat acttcttact cgcccatctg
    29521 caacggatgg gcgaatttat acccgctttc tcgtctgctg taatattccc cactacactt
    29581 ccactgttgc gtcaggcgtt tgtcgccata cgcttacagg gtggcccgca tgcaaaaagc
    29641 acaacggatc attaaaacct atcgccgtaa tcgaatgatt gtttgtacga tttgcgcact
    29701 cgttacgctc gcttcgaccc tgagcgtgcg atttatttca cagcgtaact taaatcaaca
    29761 acgggtagta caattcgcca atcacgctgt agaggaatta gataaagtac tgcttcccct
    29821 acaggcaggt agcgaagtct tgcttccgct gattggtctg ccctgctctg tcgcccattt
    29881 gccattacgt aaacaggcgg caaaactcca aactgtgcga tccattggcc tggtgcaaga
    29941 cggcacactt tattgctcca gcatttttgg ttatcgcaat gtgcccgtcg tggacattct
    30001 ggctgaactt cctgcaccgc aaccactttt acgcctgacg atcgaccgtg ccctgattaa
    30061 aggcagtccg gttttgattc aatggacgcc tgcagcgggc agtagcaatg ctggggtcat
    30121 ggagatgatt aacatcgact tactgacggc aatgctcctt gagccacaac tgccgcaaat
    30181 cagtagcgcc agcctgacgg tggacaaacg gcatttgctc tatggtaatg ggctggtaga
    30241 ttcccttccg caacctgaag acaatgaaaa ctatcaggtt tcttcgcaac gctttccttt
    30301 taccattaac gttaatggtc cgggggctac ggcgctggca tggcactatc ttccaacaca
    30361 attaccgctg gcggtgctgc taagtttact ggtgggctac attgcctggc tggcgaccgc
    30421 ttaccggatg agcttttccc gcgaaatcaa tctgggcctg gcgcaacatg agttcgaatt
    30481 gttctgtcag cctttgctta atgcgcgcag ccagcaatgt attggtgtag agattttgct
    30541 gcgctggaac aatccgcgtc agggctggat ttcaccggat gtgtttattc ctatcgcgga
    30601 agaacatcat ttaattgtgc cactgacccg ctatgtgatg gcagaaacca ttcgtcagcg
    30661 ccatgttttc ccgatgagta gtcagtttca tgttggcatt aacgtcgcac ccagccattt
    30721 tcgccgtggt gtgctgataa aagatctcaa tcagtactgg tttagcgctc acccgattca
    30781 gcaactgatc ctcgaaatca ccgaacgcga tgccttactg gatgttgatt atcggattgc
    30841 ccgcgagcta catcgtaaaa acgtcaaact ggcgattgat gacttcggca ccggcaacag
    30901 ttcattttcc tggcttgaaa cattacgtcc tgacgtgctg aaaattgata agtcatttac
    30961 cgcagctata ggttctgacg cggttaactc gacggtgacc gatatcatca tcgcgctggg
    31021 gcaaagactg aatattgaac tggtggcgga gggcgtggaa acgcaagaac aggcgaagta
    31081 tttgcgccgt catggcgtgc atattttgca agggtatttg tacgcacagc cgatgccgct
    31141 acgtgatttt cccaaatggc tggcgggcag ccaaccgccg cccgcccggc ataatggaca
    31201 tatcacgccc attatgccgt tacgttaacg cttactcatc ttcatcgtgc gccggttgct
    31261 ctttaacaat gcgaaccaga tcaacacgat aatcattggc ttcaatgatg gtgatatgca
    31321 gtggccctac atcaatcaca tcgcccacac ggggaatgtg accatttgcc gagatcacca
    31381 ggcccgcgac cgtcgcgata tcatcgtcat cggcaaggtg ctcaacatca agcgcctgct
    31441 gcaaggcatg caaatctgta ccgcctttta ccagccagcc gtcaccatcg gtaatgattt
    31501 ccggcgtttc gtcagcgtcc gggaattcac ccgcaatggc ttccagcaca tccagcggcg
    31561 tgaccaagcc ttgtaccaca ccaaactcgt tggtcacgat aacaaagctc ccgcgagcgc
    31621 gacgcagcac gcccagcagg ttgatcggat cgagggtttc cgggacgata atcgccggag
    31681 acgccgaagc aatcgccgcc acatcaacgc cctcttccag cgccaccagc agttctttag
    31741 cacgtacgat tccgatgatt tcatccagtt caccgcgaca taccgggaac aggctgtgcg
    31801 gtgaggagag cagctgctcg cggatttcat cgaccccgag attagcgtca acccagctta
    31861 tttcaccgcg cggcgtcatg atcccgcgca gagaacgcga cgccagcgtc agtacgccgt
    31921 taatcatgta acgttcttct tcggcaaatg caccttccgg gatcggcatc ggcatcgggt
    31981 tatcggcatc gtgctgaaca ttggcctgac gtttcccgcc catcaaacgc aggatggcat
    32041 cggcagtacg cgctcgcagc ggcaaagtcg actggtgacg aataaagttg cgacgcgcaa
    32101 tctggttaaa cacttcgatg atgatcgaga agccaatcgc ggcatacagg taacctttcg
    32161 gaatgtggaa accgaaacct tctgccacca gactcagacc aatcattaac aggaagctca
    32221 gacagagcac caccaccgtg gggtgctggt taacgaatcg cgtcagcggt ttggatgcca
    32281 gcaacataac cgccatcgca atcactaccg ccgccatcat caccggcaga tggttaacca
    32341 tccctactgc agtaattacc gcatccaacg agaagacggc gtcaaggatg acaatctgtg
    32401 tgacgaccac ccagaaactg gcgtagcctt taccgtggcc ggaatcatga tcgcggtttt
    32461 ccagccgttc atgcagttcg gttgttgctt tgaacagcaa gaatatcccc ccgaacaaca
    32521 taatcaggtc gcgtccggag aaggagaaat ccatgacggt aaatagcggt ttggtcagcg
    32581 tgaccatcca tgaaatcagc gacagcagcc ccagacgcat aatcagcgcc agtgataacc
    32641 ccagcaaacg cgctttatcg cgttgttttg gcggcagttt gtcagcaaga atggcgatga
    32701 agaccaggtt atcgataccc agcacaattt cgagaacaac aagcgtgagt agccccgccc
    32761 aaattgaggg gtccattaag aattccatga caagctcctg cttaaggaat agctattcga
    32821 cgccagaaat aatgcaggcg taacgacaaa atgcaaacga aaggtgcggc atagagagcc
    32881 agaaaggcag gcgttaaaag gcctgatgct gaaatgacgt cggtgacgat ccatactgcg
    32941 ggctactgcc ctatactcca tggttgttaa acgggagtta aacatatcag agacgcctct
    33001 gatttggcaa agatttacct tcctttgcaa acgaatgtga caaggatatt ttacctttcg
    33061 aaatttctgc taatcgaaag ttaaattacg gatcttcatc acataaaata attttttcga
    33121 tatctaaaat aaatcgcgaa acgcaggggt ttttggttgt agcccttatc tgaatcgatt
    33181 cgattgtgga cgacgattca aaaatacatc tggcacgttg aggtgttaac gataataaag
    33241 gaggtagcaa gtgaccattg ctattgttat aggcacacat ggttgggctg cagagcagtt
    33301 gcttaaaacg gcagaaatgc tgttaggcga gcaggaaaac gtcggctgga tcgatttcgt
    33361 tccaggtgaa aatgccgaaa cgctgattga aaagtacaac gctcagttgg caaaactcga
    33421 caccactaaa ggcgtgctgt ttctcgttga tacatgggga ggcagcccgt tcaatgctgc
    33481 cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt gcaggcgtta acattccaat
    33541 gctcgtggaa acgttaatgg cccgtgatga tgacccaagc tttgatgaac tggtggcact
    33601 ggcagtagaa acaggccgtg aaggcgtgaa agctctgaaa gccaaaccgg ttgaaaaagc
    33661 cgcgccagca cccgctgccg cagcaccaaa agcggctcca actccggcaa aaccaatggg
    33721 gccaaacgac tacatggtta ttggccttgc gcgtatcgac gaccgtctga ttcacggtca
    33781 ggtcgccacc cgctggacca aagaaaccaa tgtctcccgt attattgttg ttagtgatga
    33841 agtggctgcg gataccgttc gtaagacact gctcacccag gttgcacctc cgggcgtaac
    33901 agcacacgta gttgatgttg ccaaaatgat tcgcgtctac aacaacccga aatatgctgg
    33961 cgaacgcgta atgctgttat ttaccaaccc aacagatgta gagcgtctcg ttgaaggcgg
    34021 cgtgaaaatc acctctgtta acgtcggtgg tatggcattc cgtcagggta aaacccaggt
    34081 gaataacgcg gtttcggttg atgaaaaaga tatcgaggcg ttcaagaaac tgaatgcgcg
    34141 cggtattgag ctggaagtcc gtaaggtttc caccgatccg aaactgaaaa tgatggatct
    34201 gatcagcaaa atcgataagt aacgtattgt gttgattatc actcagtttt cacacttaag
    34261 tcttacgtaa acaggagaag tacaatggag attaccactc ttcaaattgt gctggtattt
    34321 atcgtagcct gtatcgcagg tatgggatca atcctcgatg aatttcagtt tcaccgtccg
    34381 ctaatcgcgt gtaccctggt gggtatcgtt cttggggata tgaaaaccgg tattattatc
    34441 ggtggtacgc tggaaatgat cgcgctgggc tggatgaaca tcggtgctgc agttgcgcct
    34501 gacgccgctc tggcttctat catttctacc attctggtta tcgcaggtca tcagagcatt
    34561 ggtgcaggta tcgcactggc aatccctctg gccgctgcgg gccaggtact gaccatcatc
    34621 gttcgtacta ttaccgttgc tttccagcac gctgcggata aggctgctga taacggcaac
    34681 ctgacagcga tttcctggat ccacgtttct tctctgttcc tgcaagcaat gcgtgtggct
    34741 attccggccg tcatcgttgc gctgtctgtt ggtaccagcg aagtacagaa catgctgaat
    34801 gcgattccgg aagtggtgac caatggtctg aatatcgccg gtggcatgat cgtggtggtt
    34861 ggttatgcga tggttatcaa catgatgcgt gctggctacc tgatgccgtt cttctacctc
    34921 ggcttcgtaa ccgcagcatt caccaacttt aacctggttg ctctgggtgt gattggtact
    34981 gttatggcag tgctctacat ccaacttagc ccgaaataca accgcgtagc cggtgcgcct
    35041 gctcaggcag ctggtaacaa cgatctcgat aacgaactgg actaacaggt gagcgaaatg
    35101 gttgatacaa ctcaaactac caccgagaaa aaactcactc aaagtgatat tcgtggcgtc
    35161 ttcctgcgtt ctaacctctt ccagggttca tggaacttcg aacgtatgca ggcactgggt
    35221 ttctgcttct ctatggtacc ggcaattcgt cgcctctacc ctgagaacaa cgaagctcgt
    35281 aaacaagcta ttcgccgtca cctggagttc tttaacaccc agccgttcgt ggctgcgccg
    35341 attctcggcg taaccctggc gctggaagaa cagcgtgcta atggcgcaga gatcgacgac
    35401 ggtgctatca acggtatcaa agtcggtttg atggggccgc tggctggtgt aggcgacccg
    35461 atcttctggg gaaccgtacg tcctgtattt gcagcgctgg gtgctggtat cgcgatgagc
    35521 ggcagcctgt taggtccgct gctgttcttc atcctgttta acctggtgcg tctggcaacc
    35581 cgttactacg gcgtagcgta tggttactcc aaaggtatcg atatcgttaa agatatgggt
    35641 ggtggcttcc tgcaaaaact gacggaaggg gcgtctatcc tcggcctgtt tgtcatgggg
    35701 gcattggtta acaagtggac acatgtcaac atcccgctgg ttgtctctcg cattactgac
    35761 cagacgggca aagaacacgt tactactgtc cagactattc tggaccagtt aatgccaggc
    35821 ctggtaccac tgctgctgac ctttgcttgt atgtggctac tgcgcaaaaa agttaacccg
    35881 ctgtggatca tcgttggctt cttcgtcatc ggtatcgctg gttacgcttg cggcctgctg
    35941 ggactgtaag actgttgtac actaccgggg ccttttggcc ccgttttttt atctggagga
    36001 ttaatgacaa tcacggacct ggtactgatt cttttcatcg ccgcactcct ggccttcgcg
    36061 atctacgatc agttcatcat gccccgccgt aacggcccca ccctgctggc aattcctttg
    36121 ctccggcgtg gtcgcatcga tagcgttatc ttcgtcggat tgattgtcat tcttatctat
    36181 aacaacgtca cgaatcatgg tgcgttaata acgacatggt tattaagcgc actggctctg
    36241 atgggttttt atatattctg gatccgcgtt ccgaagatca tctttaaaca aaaaggtttt
    36301 ttcttcgcca atgtctggat tgaatatagc cgaatcaaag cgatgaactt gtcggaagat
    36361 ggcgtgttgg tgatgcaatt agaacagcgt cggctgttaa tccgcgttcg aaatatcgac
    36421 gatctggaaa aaatttataa gcttctcgtt tcaactcaat aagttatgaa tttagccaaa
    36481 gctatgttta gtgtattttt a
//
LOCUS       DAZGCS010000044        36265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGCS010000044 DAZGCS010000000
VERSION     DAZGCS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.044"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..265)
                     /locus_tag="ACHK01_002258"
     CDS             complement(<1..265)
                     /locus_tag="ACHK01_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097204.1"
                     /translation="MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALP
                     VVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQS"
     gene            complement(298..768)
                     /gene="cynS"
                     /locus_tag="ACHK01_002259"
     CDS             complement(298..768)
                     /gene="cynS"
                     /locus_tag="ACHK01_002259"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO8097205.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(799..1458)
                     /gene="cynT"
                     /locus_tag="ACHK01_002260"
     CDS             complement(799..1458)
                     /gene="cynT"
                     /locus_tag="ACHK01_002260"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO8097206.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            1568..2467
                     /gene="cynR"
                     /locus_tag="ACHK01_002261"
     CDS             1568..2467
                     /gene="cynR"
                     /locus_tag="ACHK01_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO8097207.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALHELGAGKRAIHDVAALTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(2507..3790)
                     /gene="codA"
                     /locus_tag="ACHK01_002262"
     CDS             complement(2507..3790)
                     /gene="codA"
                     /locus_tag="ACHK01_002262"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO8097208.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3780..5039)
                     /gene="codB"
                     /locus_tag="ACHK01_002263"
     CDS             complement(3780..5039)
                     /gene="codB"
                     /locus_tag="ACHK01_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO8097209.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(5282..7168)
                     /gene="prpE"
                     /locus_tag="ACHK01_002264"
     CDS             complement(5282..7168)
                     /gene="prpE"
                     /locus_tag="ACHK01_002264"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO8097210.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(7208..8659)
                     /gene="prpD"
                     /locus_tag="ACHK01_002265"
     CDS             complement(7208..8659)
                     /gene="prpD"
                     /locus_tag="ACHK01_002265"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO8097211.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(8693..9862)
                     /gene="prpC"
                     /locus_tag="ACHK01_002266"
     CDS             complement(8693..9862)
                     /gene="prpC"
                     /locus_tag="ACHK01_002266"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO8097212.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(9907..10797)
                     /gene="prpB"
                     /locus_tag="ACHK01_002267"
     CDS             complement(9907..10797)
                     /gene="prpB"
                     /locus_tag="ACHK01_002267"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO8097213.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            11036..12622
                     /gene="prpR"
                     /locus_tag="ACHK01_002268"
     CDS             11036..12622
                     /gene="prpR"
                     /locus_tag="ACHK01_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO8097214.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(12720..12995)
                     /gene="yahO"
                     /locus_tag="ACHK01_002269"
     CDS             complement(12720..12995)
                     /gene="yahO"
                     /locus_tag="ACHK01_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO8097215.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            13142..13813
                     /gene="yahN"
                     /locus_tag="ACHK01_002270"
     CDS             13142..13813
                     /gene="yahN"
                     /locus_tag="ACHK01_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO8097216.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(13830..14075)
                     /gene="yahM"
                     /locus_tag="ACHK01_002271"
     CDS             complement(13830..14075)
                     /gene="yahM"
                     /locus_tag="ACHK01_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO8097217.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(14488..15303)
                     /gene="yahL"
                     /locus_tag="ACHK01_002272"
     CDS             complement(14488..15303)
                     /gene="yahL"
                     /locus_tag="ACHK01_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO8097218.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(15546..16595)
                     /gene="yahK"
                     /locus_tag="ACHK01_002273"
     CDS             complement(15546..16595)
                     /gene="yahK"
                     /locus_tag="ACHK01_002273"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO8097219.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(16682..17638)
                     /locus_tag="ACHK01_002274"
     CDS             complement(16682..17638)
                     /locus_tag="ACHK01_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8097220.1"
                     /translation="MKKSWRNNVEFYLIGLLALTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(17635..18606)
                     /locus_tag="ACHK01_002275"
     CDS             complement(17635..18606)
                     /locus_tag="ACHK01_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8097221.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(18599..20083)
                     /locus_tag="ACHK01_002276"
     CDS             complement(18599..20083)
                     /locus_tag="ACHK01_002276"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8097222.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(20132..21115)
                     /locus_tag="ACHK01_002277"
     CDS             complement(20132..21115)
                     /locus_tag="ACHK01_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8097223.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(21370..21780)
                     /locus_tag="ACHK01_002278"
     CDS             complement(21370..21780)
                     /locus_tag="ACHK01_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO8097224.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(22079..23461)
                     /gene="yahJ"
                     /locus_tag="ACHK01_002279"
     CDS             complement(22079..23461)
                     /gene="yahJ"
                     /locus_tag="ACHK01_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO8097225.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVTAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(23471..24421)
                     /gene="yahI"
                     /locus_tag="ACHK01_002280"
     CDS             complement(23471..24421)
                     /gene="yahI"
                     /locus_tag="ACHK01_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO8097226.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(24564..25982)
                     /gene="yahG"
                     /locus_tag="ACHK01_002281"
     CDS             complement(24564..25982)
                     /gene="yahG"
                     /locus_tag="ACHK01_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8097227.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(25982..27529)
                     /gene="fdrA"
                     /locus_tag="ACHK01_002282"
     CDS             complement(25982..27529)
                     /gene="fdrA"
                     /locus_tag="ACHK01_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8097228.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(27519..28382)
                     /gene="yahE"
                     /locus_tag="ACHK01_002283"
     CDS             complement(27519..28382)
                     /gene="yahE"
                     /locus_tag="ACHK01_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8097229.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(28422..29027)
                     /gene="yahD"
                     /locus_tag="ACHK01_002284"
     CDS             complement(28422..29027)
                     /gene="yahD"
                     /locus_tag="ACHK01_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO8097230.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            29285..29782
                     /gene="yahC"
                     /locus_tag="ACHK01_002285"
     CDS             29285..29782
                     /gene="yahC"
                     /locus_tag="ACHK01_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8097231.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGSKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            29874..30806
                     /gene="yahB"
                     /locus_tag="ACHK01_002286"
     CDS             29874..30806
                     /gene="yahB"
                     /locus_tag="ACHK01_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8097232.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(30848..31936)
                     /gene="pdeL"
                     /locus_tag="ACHK01_002287"
     CDS             complement(30848..31936)
                     /gene="pdeL"
                     /locus_tag="ACHK01_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8097233.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(32078..36061)
                     /gene="ehaA"
                     /locus_tag="ACHK01_002288"
     CDS             complement(32078..36061)
                     /gene="ehaA"
                     /locus_tag="ACHK01_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO8097234.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKDQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANRGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGNQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         8686 a         9808 c         8846 g         8925 t
ORIGIN      
        1 cactttgggg gtaaagctca cgcatcaatg caccgacggc aatcagcaac agactgatgg
       61 cgacactgcg acgttcgctg acatgctgat gaagccagct tccggccagc gccagcccgc
      121 ccatggtgac caccggcaga gcggtcaaca gggcagccac gctaaagctc attccgctcg
      181 cctggcgcaa ttgcggtagc agtggcccga cggaggtgag cagtggtcgc atattaagac
      241 caatcagcac cagaaccagc agcatcgtga acgtcttttg atgattgcgc atggctgtca
      301 gaacggcttg gtcggcagat atttaccatc taaggtaatg accgcacgtt cgccaccttc
      361 cgggtccgcc actttcttaa cgtcgagttt gaagttaatc gcgctgataa tgccatcgcc
      421 aaacttctca tgaaccaacg ctttcagggt tgtgccgtac acctgcaaca tttcatagaa
      481 acgatacatc gttgggtcgg ttggaatacg gtcatcaatg cagccacgca gtggaatcat
      541 ctgcaacagt agaatggcgt cttcgtcgag atccagcttc gccccgacca ggcgggcggc
      601 gtcggcagga agcgcttgct gccccagcaa agccgcggtt acgaaggctt ctgccagacc
      661 ggtaccgtcg gcaatctcgg caaatgagag atctttttta gctttgctga gcaaaatggc
      721 atcggcaaga tcaagacgaa tattgcggtt aatttgtgac tgaatcatgg tggatctcct
      781 gatggtttaa aaataaggtt acgctgcggt cggttggcgt agcggtatgg cacaaacgcg
      841 aggattagcg gccagtggca caaactggcg ggttgcgccg tcaaaagctg cgatgctgcc
      901 gctttcaatg tcgtagaccc agccgtgcag ggcgatccgc ccctcttcga gcgccaggcg
      961 caccgatgga tgagtttgca aattagccaa ctgagcaatg acgttttcac gtaccatcgc
     1021 cgcagctttt gacggtaaat cggaatgcgg gcgcgcctca ttaacgacgc gggctgaatc
     1081 ggcataacgc agccagtggg agacggcagg catatggtcc atgcactgac agctggcaat
     1141 ggcggtcatc gcgccacagt tggaatgacc acaaatcaca atgtcagata cccgaagcgc
     1201 agcgacggca tactccaccg aagcagaaac gccaccgggt tccggcccgt aggaagggac
     1261 gatattgccc gcgttgcgaa taacgaacag atcgccaggc tcacgttgcg tcaccagctc
     1321 agggaccaga cggctgtcgg agcaggagat aaaaagtgtg cgcgggcttt gctgtgtcgc
     1381 cagctgttta aacaaggctt cccgcttcgg aaatgcctcg cgctggaatt taaggaatcc
     1441 atcaataatc tctttcaccg ttaacctctg tctgtctctg gaatgagagg cagactactc
     1501 gccgattgtc ataaggtaaa agtctcattt atgatgagtt ctattggatt tacttatagg
     1561 ttggcgaatg ctctctcgac atatcaatta ctttcttgcc gtggctgaac atggcagctt
     1621 cacccgtgcc gccagtgcgt tgcacgtctc ccagcctgcg ctttcccagc agattcgcca
     1681 gttagaggag agtttgggcg tgccgctgtt tgaccgtagc gggcgaacga ttcgtctcac
     1741 cgatgcagga gaagtctggc gacagtacgc cagccgggcg ttacatgaac tgggagcggg
     1801 taaacgggcg attcatgatg ttgccgctct gacgcgagga tcgctgcgta tcgccgtcac
     1861 ccccaccttt acgagctact ttatcggccc cttaatggcg gatttctatg cgcgctaccc
     1921 cggcatcacg ctccagctac aggaaatgtc gcaggagaaa atcgaagatc tgctttgtcg
     1981 cgacgagctg gacgttggga ttgccttcgc gcctgtgcat tcgccggagc tggaggcaat
     2041 tcctttactg acagaaagtt tagcgttggt ggtggcgaaa catcatccgc tggctgcctg
     2101 tgaacaggtg gcgttgagtc gcttgcatga tgaaaaactg gtcctgctca gcgcggaatt
     2161 tgccaccaga gagcaaattg accactactg cgagaaagcg gggctacatc cacaggtggt
     2221 cattgaggcg aactcaatta gcgcggttct ggagctgatt cgccgcactt ccctttccac
     2281 attgttacca gcagcgattg ccacacaaca tgacgggctt aaagcgattt ctcttgcccc
     2341 gccactactg gagagaacgg cggttttgtt gcggcggaaa aatagctggc agactgccgc
     2401 tgcgaaggca tttttgcaca tggcgttgga ggaatgtgcg gatgttggcg agaatgaatc
     2461 acggtagcca ctgacacagg tcagtggatc ttcaacacag aggaaattac cgtttgtaat
     2521 cgatggcttc tggctgctcc agatatacgg tggtttgtgc cggttgtgta ctggcaatca
     2581 ccttgccgcc acgtaccgaa taacgtaccg gaacctgacg gcgcagcgca tcaaatccat
     2641 tttcagccgg caggataatc aggtttgcgc tgtttccggc ggcaatgccg taatcctgca
     2701 aattcaatgt tctggcactg tggtgggtga ttaaattcag gccatcgtta atctgcccgt
     2761 agcccatcaa ctggcaaaca tgcagcccca tatgcagcac ttgcagcata ttcgccgttc
     2821 ccagcggata ccacggatcg aagacatcat cgtgaccaaa gcagacgtta atgccggact
     2881 ccagcatctc tttaacgcgc gtgatgccgc gacgttttgg atacgtatcg aaacgtcctt
     2941 gcagatgaat attgaccagc gggttggcga caaagttaat accggacatt ttcagcaagc
     3001 ggaacaggcg tgaggtatac gccccgttat aggagtgcat tgccgtggtg tggctggcgg
     3061 tgactcgcgc gcccatgcct tcacggtgcg ccagggcagc aacggtttcg acaaagcgcg
     3121 actgctcgtc atcgatctca tcacagtgaa cgtcgatgag acggtcgtat ttttgcgcca
     3181 gggcgaaggt tttatgcagc gactccacgc cgtattcacg ggtaaattca aaatgcggaa
     3241 tcgcccccac tacatctgcc cctaagcgta acgcctcttc cagcaacgct tcaccgttgg
     3301 gatacgacaa aatcccttcc tgagggaagg cgacaatttg cagatcaatc cacggcgcga
     3361 cttcctgctt cacttccagc attgctttca gcgcagttag cgttgcatcc gaaacatcga
     3421 catgggtacg cacatgctga atgccgttgg caatctgcca tttcagcgtt tgccaggcgc
     3481 gttgtttcac atcgtcatgg gttaataacg ctttgcgctc ggcccagcgt tcaatgcctt
     3541 caaacagcgt gccggactga ttccagttcg gttgtccggc ggtttgcgtg gtgtccaggt
     3601 gaatatgcgg ctccacaaac ggcggtataa ctaaaccttg ttcggcatcc aggctgtttt
     3661 cagttatggg catcacgccg gattgcgcat caatggcgct gatttttccg tcctgcagat
     3721 gaatctgcca cagcccctct ttgcctggta accgggcgtt aataattgtt tgtaaagcgt
     3781 tattcgacac tgttaacctc cacatgcgtc attgctgctg tcgttttacg attcaaaatc
     3841 gggttaagga tcagatagct cagcgcgcca cctaataccg cgttgaccgg aacaattccc
     3901 ggtaaccagt ggcctgcggc aatccccagc gcgaccgcca gaatcgccac ccaattgaca
     3961 ctcatcatac gcgtggtcgc aaagtgctca tagcggcgac ggttcatcag atagtcggcg
     4021 atgatcacgc cccctactgg aggaatagct gccgaaagga aggtcaacca gccgacaaaa
     4081 ttgttataca gccataatgc gcagaccgta ccgataatac cgttgattac cgaaagggtt
     4141 ttgctcgaca taccggtaat gttggcgaaa cctaaacccg acgcatagag tgcgttatcg
     4201 ttggtggtcc agatattcag ccccagcacc acaatcgcag gcagcagcag gccctgagca
     4261 atcatcacat cagagatatc cgccatgccc agtgccgcag cgcccgctgc accgaaaata
     4321 aacatcaacg agttgccgag gaaaaaggcc accatcgcca ccagcaccgc cagtttggca
     4381 ttgcgaccaa accggacaaa gtcagcggtg agcgtacccg cactgataaa tgaccccaca
     4441 accagcgcca gcgcgacatt gaaatctaac ggttgtgcgg gaacgaccgc ttttaatgcg
     4501 tccaggccgc ccatgccgtt aacagccagc cacacggaat aaccgcccag gcaggcgata
     4561 gccggaaccg caatcaccga aagaaccgtc agcgccgaaa tgccaaaaaa gacggtgacg
     4621 gtcatcagta aaccggaaac ggcaatcagc aaattaatat ccagcccggt tgccttaccc
     4681 accggaatgg caaacatcgc cacaccgacg ccaaaccagc caacctgagt tccgcccagt
     4741 agcagtgaag gcagccatga gcctttaaca ccaaacgaga agcgagcaag aagatgagtg
     4801 gtcaggccgg tttttgcgcc aatgtaaccg agaaatgaag tgtaaatacc gaggagaaga
     4861 ttaccgatga gaactgcgag gaagaaatca tgatagctaa gaccggttcc gagagtgccg
     4921 ccggtccaca tactggcgga aaagaaggtt aatcccagca tgacgaacgt caatgccaat
     4981 acccctttcc gcgccgactg cgggactggc ccctggctaa agttgttatc ttgcgacacg
     5041 aaattccccc tttttgctgt ttgaaaccca aaaaatccgc cgcattctat tcatctgaag
     5101 ataaaaagca atcgttttcg tggggaaata tattttttat atgaatgagt gcaaaaaggt
     5161 gggggaaaaa ttatcggtgt aaaaacagtg ggtagtgggt aggcctgata agacgcgtca
     5221 gcgtcgcatc aggcatcggc gcatggtgtc ggatgcgcca cgcgacgcat ccgacaatac
     5281 cttactcttc catcgcctgg cggatctgat ccaatgacgc aggatcatca atggtcgtca
     5341 gatccccagg atcgcgtcct tcgcaaattg cctggatcgt gcggcgcagc atttttccgg
     5401 atcgcgtttt cggcaattgc gagacaaacc agacgtgcgc cgggcgacca aagttgccaa
     5461 tctggctgtc caccagcgcc ataatcgcct tctcttgcga atgcgccaca tcacgatctt
     5521 ccagactatc gctctctttc ggaatgacaa acgccaccgc cacctgccct ttcagcgcat
     5581 ctttcacccc aaccaccgcc acttcggcaa cgcccggatg gctggagata ctctcttcaa
     5641 tctcgcgcgt gcccagacga tgcccggcaa cgttaattac atcgtcagtg cgcccgagaa
     5701 taaagtgata accgtcagcg tcacggatgc cccagtcaaa ggtggcgtac accgggcggg
     5761 aaaacagcga ccagtaagtc ttcacaaagc ggtcgtcgtc gccccagatg gtctgaatac
     5821 agcccggcgg cagcggccct tccaccacca gcatcccttt ctcgttgacg ccacacggtt
     5881 cgccggtgac ttcattaagc aactgcacgt tatagccata catcggcacg ccggggcttc
     5941 ccagacgcgt cggcctgtcg tccagaccgc gagcaatcgc cataatcggc cagccggatt
     6001 cggtctgcca gtagttgtcg atgaccggca catccagcgt attgctcacc caactggcgg
     6061 tcggctcgtc cagcggttct ccagccagat agagcacttc cagcgacgag agatcgtgtt
     6121 tgcgaatttc agcggtaggg aattttttca gcacgcgaat ggcggtcggc gctgagaaca
     6181 tccggctaac ctgatatttc tcgacgattt tccaccacac gccgcagtcc ggccaggtcg
     6241 gcaatccttc gtaaacgata gtcgccatcc ccgccagcag cggcgcgtaa acgatatacg
     6301 aatgccccac cacccagccg atatccgatg cgcaaaagaa cacgctgccc gctttgccgc
     6361 caaaaatggt gtccatcgag gtcgccagcg ccaccgcata tccgccgacg tcacgctgca
     6421 cgcctttagg tttgccggtc gtgccggaag tgtagagaat gcaggaggtt tcgttggatt
     6481 ccagccacgc caccggtacc cgcgcgccga tgtgttgatg gcgcaacgac gcgaaatcga
     6541 catcccgccc gctgacgcgc gccattttcg ccagcccgcg atccaccagc aaaacatggc
     6601 gtggctggtg ctgcgcctga cttatcgcat cgtcgagcaa ttttttatag ggaatgattt
     6661 tgccaccgcg cgctccggca tcagccgaga caatcagcac cggtttagcg tcatcaattc
     6721 gcgccgccac gctgtgcgag gcaaaaccac caaataccac cgaatgaata gcaccaatgc
     6781 gcgcgcaggc cagcagagta atatgcgctt cggcaatcat cggcatatac accagcaccc
     6841 gatcgccgcg ctgcacgccc agtgagcgca acattgaggc caccgcgttc acttcgtcat
     6901 gcagctggcg aaaggtaaag gtgcgctctt cttctgtttc cgaagagacg gcaatcaatg
     6961 ccagtgcctc tggctgtttc tccagccagc ggtcgatagc gttgtggcac aaattggttc
     7021 ggccttcaca aaaccaacgg gcaaacggcg gattgctgtg gtcgagcgtt tgcgtaaagg
     7081 gcgtctgcca gtcaatacgc cgggcctgct cggcccagaa ctgctccggt tcgttaatcg
     7141 aacgctgata aaattcgcta aaagacataa tgctctcctg ttgaacttac gccttaccgc
     7201 cgtttactta aatgacgtac aggtcgagat actcattgac cggcatctgt tccaggcgag
     7261 ttctgtcgag agaaacctcc agaatgcgct gctgttggcg agtcgggaac tggcgcgcga
     7321 gattgatttt gaatttatcg accagtttcg gaataccatc ctgacggcgg cgagcatgac
     7381 caatggggta ctccaccacc acttcttcaa atcgtgtgcc gtcggtgaac tcaagggtaa
     7441 tggcattggc gatggcgcgt ttttccgggt cgtggtagtc agcggtaaat gccggatctt
     7501 caaagcaatt gatcttctcg cgcagggcgt cgatgcgttt atcttgcgca acgttgtcct
     7561 cgtaatctgc cgccgttaag cgcccgaata acagcgggat cgccaccatg tactgaatgc
     7621 agtggtcgcg gtcagccggg ttattgagcg gccccttttt gtcgatgatg cgaatacagg
     7681 cttcgtgggt gcgaatggtc actttttcga tatccgccgc cgtcttgcct gctgcctgca
     7741 tctgttcata gagcgtcatc gccgcttcaa ctgccgtctg ggagtggaac tccgccggga
     7801 aggagatttt gaacagcaca ttctccatga cgtaagaacc gtacggacgc tggaagcgga
     7861 acgattcacc tttaaaggag acgtcgtaga agccccatac cggcgcagtc agggctgacg
     7921 ggtaacccat ttcgcccgtt ttcgccatca gtgccagacg taccgcgcgg gaagtggcat
     7981 cgcccgccgc ccaggattta cgcgtgccgg tgttcggcgc atggcgatag gtgcgcagcg
     8041 actgaccgtc cacccacgcc agcgaaacgg cgttgagaat ttcctcgcgg gtcaggccga
     8101 gcatttcggc gaccacggcg gtggaagcca ctttcactaa cagaacgtgg tcgaggccga
     8161 cgcggttaaa ggagttttcc agcgcgatgc agccctgaat ttcatgggct ttgatcattg
     8221 cggtcagcac ctgtttcatg gtcaacggcg ctttgccgct ggcgaccgcg ttgcgcgaaa
     8281 gccagtccgc cgttgccaga atgccgccga ggttgtcgga aggatggccc cactccgccg
     8341 ccagccaggt atcgttgaaa tcgagccagc ggatcatcgc gccgatgtta aatgccgcct
     8401 ggacggggtc gagctggaac tgagttccgg ggacgcgcac gccgttgggt acgacggtgc
     8461 cgggaacaat tggccccagc agttttttac aggccgggta ttcgagagct tccagaccgc
     8521 agccgagcgt gtcgagcagg cagtaatgtg cggtgtcgta ggccacttta gagctgattt
     8581 cgtagttcat gacgtaatcg acgatatcaa cgatttcacg atcaaattcc gggcggatgt
     8641 tgttgatttg agctgacatt gggtacgttt ccttattgtt attcgtagag gtttactggc
     8701 gcttatccag cgcgacaaac gggcggtctt ccggtccaac ataattggcg gaaggacgga
     8761 taattttgtt gtcctgacgt tgttcgataa tgtgcgccgc ccagccggtg acgcgggcga
     8821 taacaaacag tggtgtgaac atctcggtgg gaacgcccat catgttgtag gaaacagcag
     8881 agaaccagtc gagattgggg aacatctttt tgctctccca catcaccgtt tccaggcgat
     8941 cggcgatgtt gtacatcttc agcgagccgc cttcctgcga gagctgcttc gccacacgtt
     9001 taatcacctg gtggcgcggg tcagcgatgg tgtaaaccgg atgaccaaaa ccaatgacca
     9061 cttctttgtt ttccacgcgc ttgcggatat ctgcttcggc ttcgtccggc gtttcgtagc
     9121 gttgctggat ctccagcgac acttcattcg ccccgccgtg ttttggcccg cgcagtgcgc
     9181 caatcgcgcc aataatcgcg gaatacatat cagagccggt gcccgcaatc acccgactgg
     9241 taaaggtgga ggcgttaaac tcgtgttcgg cgtacagcac cagcgagata tgcatcgcct
     9301 tttcccagct ttgcgatggc ttttcgccgt gcagcagatg caggaagtga ccgccgatgg
     9361 agtcgtcatc ggtttccggc tggatgcgtt cgccgttgtg actgtagtga taccaataaa
     9421 ggagaatcga gctaagtgat gccagcagtt tgtcggcaat atcccgcgcg ccagagacag
     9481 tgtgcccctc tttttctggc agtgtgcagc cgagcgcgga aacgccggtg cgcatgacat
     9541 ccatcgggtg cgacgccgcc ggtaaggctt ccagcacggt acgcacatta gctggtaaac
     9601 cacgcagggc tttcagtttc gttttgtaag cggcgagttc gtcacgggtc ggcagtttgc
     9661 cgtggatcag caggtgcgcc acttcttcaa attcgcaatg ttccgccaga tcaagaatat
     9721 cgtagccacg gtaatgcagg tcattgccac ttttacccac ggtgcagagc gccgtattgc
     9781 ccgccggaac gccagaaagt gctaccgatt ttttcggttt aatgacatgg gtactgtttt
     9841 gcaggatcgt tgtgtcgctc atattgtcct cgtcatttgt agggtcgaga tatttttcgt
     9901 tgtgttttat ttcacctggc tacgggcaaa caggtcgtcg agcttctctt cgtactggta
     9961 gtagttgatg ctttcgtaca gctcgttgcg ggtttgcatg gtatcgatga cgcttttctg
    10021 cgtgccttcc tgacgcagga cgttgtagac atgttcagcg gcgcggttca tggcgcggaa
    10081 cgctgaaagc gggtacagcg ccattgcgac atgggcgctg cgtaattcgt cggtggtaaa
    10141 cagcggtgtt gcgccaaatt cggtaatgtt ggcgaggatt ggcacctgca ccgcatcggc
    10201 aaactggcgg tacatggcga gttcggtaat cgcctccggg aacaacatct cggcacccgc
    10261 ttcaacatag gcctgcgcac gctcgatcgc cgcatccagc ccctctaccg ccagagcatc
    10321 ggtgcgcgcc atgatcacaa aatcaggatc ggttttcgca tccaccgccg cgcggatccg
    10381 atccaccatc tcttctttcg agacgatcgc tttattcgga cgatgaccgc agcgtttcgc
    10441 accaacctga tcttcaatat gcaatcctgc cgcaccggct ttaatcatcg atttcacggt
    10501 gcgcgccacg ttaaaggccg aagaaccaaa accgatatcc gcatccacca gcagcggcag
    10561 cgaacaaacg tcggtgatac ggcgaatgtc ggtcagcaca tcatcaaggg tagaaatacc
    10621 gagatcgggc agccccagcg aacctgccgc cacgccaccg ccagaaagat aaattgcctg
    10681 atatccggca cgctgcgcca acagcgcatg attagcgttg atggtgccaa caatctgcaa
    10741 tggattttct ttagtcagtg cagcgcgaaa cgctttacct ggagagtgta gagacatagc
    10801 ccatcctttg ttatcaactt gttatttgcg ttgataaaga caaagcaagg ggtgtgccac
    10861 aattgcctta ataaaattaa atgtttaatt tcaatttgtt agagataaag tcatgaacta
    10921 atccgcaaat atgcgtttca gttaacgttt caggcaatgt ttcaccgcgt ttcattgcaa
    10981 caattatgaa acaagactaa acccaatatt cggtttctta actttgcggt gcgctatggc
    11041 acatccacca cggcttaatg acgacaaacc ggttatctgg acggtatctg taacgcgcct
    11101 gttcgagctg tttcgcgata tcagcctcga gtttgatcac ctggcgaaca ttacccctat
    11161 tcagcttggc tttgaaaaag cagtgaccta catccgcaag aaactggcaa acgaacgctg
    11221 tgacgccatc atcgcggctg gctctaacgg cgcgtacctg aaaagccgcc tgtcagtgcc
    11281 agttattttg attaaaccga gcggctacga tgtgttacag gcactggcaa aagccggaaa
    11341 actcacctct tctatcggcg ttgtcaccta tcaggaaacc attccagcgc tggtggcgtt
    11401 tcaaaaaacc tttaatttgc gcctcgatca acgtagctac attaccgaag aagacgcacg
    11461 cgggcagatt aacgagctaa aagctaacgg caccgaagcg gtggtcggcg cggggctgat
    11521 taccgatctg gcagaagaag ccggaatgac cggaattttt atctattccg ccgccaccgt
    11581 gcgccaggcg ttcagcgatg cgctggatat gacgcgcatg tcgttacgcc ataacactca
    11641 cgatgccacc cgcaacgccc tgagaactcg ttacgtgctg ggcgatatgc tcggtcaatc
    11701 accacagatg gaacaagtac ggcagactat tttgctgtat gcccgctcca gtgcagcggt
    11761 gttgattgag ggggaaacgg ggacgggcaa agagctggcg gcccaggcga ttcatcggga
    11821 atattttgcc cgccacgatg cgcgacaggg caaaaagtcg catccgtttg ttgcagtcaa
    11881 ctgcggggcg attgccgaat cgctgctgga agcagaactg tttggctatg aggaaggggc
    11941 gtttaccggc tcgcgacgcg gcggtcgcgc cgggctgttt gaaatcgccc acggaggtac
    12001 gctgtttctc gatgagattg gcgaaatgcc gctgccgttg cagacccggc tgctgcgggt
    12061 gctggaagaa aaagaggtca cccgcgtcgg cgggcatcag cctgttccgg tggatgtgcg
    12121 ggtcattagc gccactcact gcaatctgga agaagatatg cggcaagggc agtttcgccg
    12181 tgacctgttt tatcggctga gtattttgcg tctgcaattg ccaccactgc gcgagcgggt
    12241 ggcggatatt ctgccactgg cggaaagctt tttgaaagtg tctctggcgg cgctctccgc
    12301 cccgttttct gccgcattac gccagggatt acaggcaagc gaaaccgtgc tggtgcacta
    12361 cgactggccg ggcaatattc gtgaactgcg caatatgatg gagcgactgg cgctattttt
    12421 aagtgtggaa ccgacgccgg atttaacgcc gcaatttttg cagctgctac tgccggaact
    12481 ggcgcgcgag tcggcgaaaa ctcccgctcc acgcttactg acaccacaac aggcactgga
    12541 gaaatttaat ggcgataaaa cagcagcggc gaattattta ggcatcagcc ggacgacgtt
    12601 ctggcggcgg ctgaaaagct gaactgccga atgcagcgcg aacgctttat ccggcataaa
    12661 tttcaataga tgatcaccga taaacttacg catcgggcat gtttacatag ggttcagaat
    12721 tacttcttct tataaatatt tgccgtgccg tggatcttat tgtcagtttg accggaggtc
    12781 agtaccaaca catcagcccc tttttcatcc gcttttttga tcaaatcttc tttcgcgtct
    12841 gcagtcgaca tttcattgct ggttgaaata tcacctattt tttcatactg cgattcaact
    12901 ttttcaaatt ccgctttggt catcaattca gcggcataga cattggtaac ggctaacgct
    12961 aacgcaccga ctaacatttt agagattatt ttcatattac ggctccagag ttgttatcac
    13021 actatcaccc acccacatta ttgggttgat taataattag ttcagtcagg gcataaacgc
    13081 cagtaaccgg aaataagaca ctttgtgatc tctctcgcac ccgattattt ttggtagaaa
    13141 tatgatgaag ttacttcact tatttatgga tgaaatcact atggatcctt tgcacgccgt
    13201 ttacctgacc gtaggactgt tcgtgattac tttttttaat ccgggagcca atctctttgt
    13261 ggtagtacaa accagcctgg cttccggtcg acgcgcaggg gtgctgaccg ggctgggcgt
    13321 ggcgctgggc gatgcatttt attccgggtt gggtttgttt ggtcttgcaa cgctaattac
    13381 gcagtgtgag gagatttttt cgcttatcag aatcgtcggc ggcgcttatc tcttatggtt
    13441 tgcgtggtgc agcatgcgcc gccagtcaac accgcaaatg agcacactac aacaaccgat
    13501 tagcgccccc tggtatgtct tttttcgccg tggattaatt accgatctct ctaacccaca
    13561 aaccgtttta ttttttatta gtattttctc agtaacatta aatgccgaaa caccaacatg
    13621 ggcacgttta atggcctggg cggggattgt gctcgcatca gttatctggc gagtctttct
    13681 tagtcaggcg ttttctttgc ccgctgtgcg tcgtgcttat gggcgtatgc aacgcgttgc
    13741 cagtcgggtt attggtgcaa ttattggtgt attcgcgcta cgcctgattt acgaaggggt
    13801 gacgcagcgg tgagcagata atttactctc tacgtaatca acctgatttg ccgaatcccg
    13861 gccatcggcg atgatagccc ccgtaatgcg tggtccttat ttccgataag tttacttgag
    13921 tctttagctt cccatttcag cccactctcc cctgtgtggg cttttttcat tggtctgacc
    13981 cgtcctgaat agccaaacaa tggcaatgtt ccactttgca gggatgataa taaggggatc
    14041 tgattcagaa gagttttgaa aagttggacc gccatcgagg cctcgaaccc cgtaccatac
    14101 aacaatcctg gttgtcgtct gctcttcctg atgagctaat ggcggttttt gatatgagcc
    14161 ttacagcata ttttctgtca ggcttgagtg cgatgcgtaa gatacagaaa agaccccaca
    14221 caaacatgat ggaattatga tttttttcat atgaatgtca acccacacat tcacaaaaca
    14281 tctgcatgag atcatttttt atacaccgca tggctgcgaa accactactg gtgctttttt
    14341 aagcgaagtg tcgccgatca caacaaaata tcggcgaaaa aagagatgga atgccagttg
    14401 gcatacagat atacagatga aatgattacc tgcattgcga ttcactctca ggtggaataa
    14461 caacttgcag gtggaaccct ttctgaacta aagagtgtag cggattttaa cgtgcttatt
    14521 tacaggataa tatctgtcac ctaattcctg gactctatta gatatccatt gatttaatcg
    14581 cgcatcatct ctgtatgttt ttacgtccag aacagagagc aataacatca ccagtgattc
    14641 aggaaaaaca gggtagatat tttttaagtg taataataat ccatgcgcaa gcgcctgctt
    14701 aatgggcgcg tctgtctccg gaatagacga catttcgcga agccattttt ccacatgcat
    14761 acactctttc cctttaggga aaattctcag caaaggcata tggtgttcaa gaagaataca
    14821 tttgcaatct gttcgcactg aatcgataaa ctgcactaat ttcttaagcc cggtaaagcg
    14881 tccagcctgc acgtttgctt taatatcaag aaacgcatca tcctggtcat atacccaaac
    14941 tgtccctttt tgattttcaa gtatgcgaaa aacaaactta accgggtgat cagggtaatc
    15001 catccagtca taaaatccag ggaaatcatt aaactgcaca tttatggtga gtgcgttatc
    15061 gtctgaccac tcacagtctc gtacggcagc catgagatga atgagtttgt cgtcatcgac
    15121 taaatcatca cgacaatcat aaggataatc ataatattgt ttaatcaaaa ccttgaatat
    15181 atcgacacag gaaaccaggg agcttttgat atcctctggt aactgattgt ttttaaccgt
    15241 attaagtatg ctttgttgcg tatatggcat caaattattg tgcggagctt ttaatgatat
    15301 catcgcgatc tctcttaaat aaaaataaac gtcgcagata ataaagctct gattaaacca
    15361 ggcactatca gaaatcgctc ataactaacg tgtgaagtat tgtgtactgg agggagttaa
    15421 tttaataatt tgaatttacg ttataggttt tactgctact ttgatattat ttcgctctcc
    15481 ttaactaaaa agttaagtgg agagcgaagt taatatgtta aaccacagcg tatttattaa
    15541 ttttttcagt ctgttagtgt gcgattatcg ataacaaaac gatatttcac atcaccgcgc
    15601 agcattcgct cataggcttc attaatttga tcggcccgaa tcatctctat atcagccaca
    15661 atgccatgtt cggcgcaaaa atcgagcatc tcctgagttt ctggaatgcc gccaatcata
    15721 gaaccggcta tcgcacggcg tttcatgatc aggttgaaaa cttccggcga tttatgcggt
    15781 gtcgcaggcg caccaaccag cgtcatggtg ccatcacgct tcagcaaggt ggtaaaatcg
    15841 tcgagattat gtggcgcagc tactgtattc aaaatgaaat cgaaactctt cagatgagcc
    15901 gccatctcat cggcattgcg tgagttaaca acttcatcgg cccccagggc ttttgccgct
    15961 tcgcgttttg cctcagaagt ggtaaatgcc accacatgtg cccccatcgc gtgggccagc
    16021 ttaatcccca tatgtcccag accgccgatg ccgaccacgc ccactttttt acccggcccg
    16081 gcctgccagt gacgtagcgg cgaatacgtg gtgatccctg cacacaacaa aggagccacc
    16141 gccgccagct gctcttgcgg gtgacgaata cgcagaacat atcgctcatg aacgacgatc
    16201 tgttgtgagt agccgcccag agtatggccc ggttcgtccg gcgtcggcga gttataggtg
    16261 ccggtcatgt gatcacagta gttttccaac ccgtcttcac actcttcgca atgtttacaa
    16321 ctgtcgacaa tgcagccgac accgaccaga tcgcccggcg catatttttc tacctgatca
    16381 ccaacggcta ccacacgccc cacaatttca tgacccggca cgcaggggta aaccgtcccc
    16441 gcccactcgg aacggacctg gtggagatcg gaatggcaaa cgccacagta agcgatttcg
    16501 attttgacat cattcggtcc cggttcacgc cgggtgatat ccatcggttc aagtggttgt
    16561 ttagcggaat atgcaccaac agctttgatc ttcattgtgt ttactcctga ttagctatgt
    16621 gtatttggcg acgcctgata tgtcatcgcg gcaaaacgcg tccattgaat atagccaata
    16681 tttaaaccac ccgatccagc cccaacatgc cgcgagcctg aatgaaacag agcagcaacg
    16741 ttccccataa ggccatcgtc aggtaggggc ttataccgag aatattgaag ccgctttcaa
    16801 taatttgcag aagaaacagc gccagcacca taccaattat ccgcccgctg ccgccgtccg
    16861 gattaacccc gccgagtact gccgcgagga ttgacaccag caagtacgat tcgccataag
    16921 aggccttagc cgagttgagt ttcgacatca tcaaaaatgc cgctaccgca caaagcaagg
    16981 cggaaatgac ataaacccaa atcagcaccc gacgagtgtt aatgccggag taaagtgttg
    17041 ctcgttcgtt agacccaatc aggtaaatgg ctttaccgag cggcgttttc tccagcaata
    17101 tccatagccc cagtgccact gcggcaaaca accacatcgg tagcggaata ccgaaccatt
    17161 gcgcatgatt caaccacagc acccatgagg gataattggc aatggcgcta ccacctgtca
    17221 ccagaatatt tatccctttg agcaacgtca tcatcccaag cgttgcaaga attggcgaca
    17281 ctcgaatacc agcaatcaat acgccgttac ataaaccgat tatcaccgcc gcgccagcgc
    17341 ccgccagtaa cgtagcgaca accgtagcaa tacccggtgg atactgagtc gccacccacg
    17401 ccatcaccag tgaacaggcg ttagcagtgg ctataattga caagttaatg ccaccgcaaa
    17461 gcatggtcac cgccatagcc aacgccagaa tgcccagcac gggcatttgc gaagcgacgg
    17521 actggaaatt acttatcgac cagaaaatct ccggcatagt aatgctgaac gcggcaacag
    17581 ttagcgccag caggccgatc aagtaaaact caacgttatt acgccacgat tttttcatag
    17641 cagactccgg ttctgatgct gactccacgc cgtggcacta atgctggcaa cgatgatgat
    17701 gccggtgatc aatgtttgcc agtaagacga gactcccaat aaatttagcc cgttttgcat
    17761 cactgccaac aacaccacgc cgagcaaagt accggttaac gtgccgcgcc cgccgagcaa
    17821 actagtgccg ccaagcacca ccgcagccag tactgtcagc tcataaccca gaagagaatc
    17881 gggggcgaca gtcatcaccg tccacgactg cactacaccc gcagcgccag acatcaatcc
    17941 catatagcca tagacgaaaa gttgcagttt cagcacgcta aagccgatgc gactggctga
    18001 ttcgcggttc ccgccaaggg cataaatttt gcgccctaca ctggtgaaat tcatgataaa
    18061 cgctgttagc agcaccaccg taatcatggc gatcagcggc agaccaaggc catagtcata
    18121 gccatcagca tcggtgtact tgaacaacat aaccccctgc tcaaaccacg gcggaaagtc
    18181 gtacagccac acacctttac tcaaccataa cagcaggcca tagaaaatat tcagcgtcga
    18241 gatagtgatg atgatagaag gcacccgtag gcgattaacc agtaaggcgt taattaagcc
    18301 aagtaaaatg ccgatgcagc ccgccagtgc aaacgcgaca gcgaagttgc caccaatttt
    18361 ttgcagcaac agcaccatgc cgtattgcgc aatgatggtc attgctggaa acgaaatatc
    18421 aattccaccg gaaatcaaca ccacaaacaa accacaggcg agaatagtca acatggcata
    18481 gttattggcg agatcgtaca aattaccgaa tgtcagaaac tcgtcgctgc gccaggcaag
    18541 gccaacaaac agcaccacaa tcagtaatgc cagccagaat tcatggcgtt ttttcagttc
    18601 agccatttac cacctccgca atcctggcct gagaactgct gtcaggcagg aagctatggg
    18661 tgatctgacc tttttgcatt accaaaatcc ggtggctttg ataccacgct tcttcaattt
    18721 catcgcagat catcaatacc gcaatgccat gagccgccag atcgctgata atgccgtata
    18781 ttcctgcttt gttggcgata tcaacaccga ctgtcgggct atcgagaatc aacagtcgtg
    18841 gcccaatcgc cagccatttg gcgatcgata ctcgctgcgc gttaccacca gaaagcgtat
    18901 tcaccggaag atgaggatca gagactttga tggtcaatgc cttgattagc ctgtccacca
    18961 ggtcacgaac tttagtttca ctcagaaagc cgaagcgatc ttttaccttg tgaaaaacag
    19021 tgctaatgat gttgtcttca atagattgcg ccatcaccaa accgcgcgac atgcgatctt
    19081 ccgaaacata accaataccg tggcggatag cgtcctggtt actatgcagt gtaaccggct
    19141 ggccgttgat gaggatctcg ccagcatccg gtcgggtcat accaaacaga ctcagacata
    19201 attccgtgcg ccccgcacca agcaggccaa caatcgatac cacttcgccc gcttccaccc
    19261 gcaagttaat atttagatat tccccatgac gacttaaatt acgcacctcc agcaccgggg
    19321 tgctggattt tccctgccac aactctcgca cctgatattc aaactcctgg ccggtcatca
    19381 ggaaaccgag ttgttttgtg gtcatctctg cagccggaaa agtcccgacc agctcaccat
    19441 ctttcagcac tgaaatacgg tcggaaactt ccatcacttc ttccagacga tgactgacga
    19501 agaccacgca gatcccgcgt tcacgcaatt gatgcaccac ctgtaataac ccttgcactt
    19561 cctgatgggt cagcgaagcg gttggttcat ccatcacgat caaccgtgcg tcctgcgcca
    19621 gtgcacggca aatagccacc agctgacagc gggcaattga aagctcagaa acgagtgtat
    19681 ccagtggcaa tgtgacgtta atactggtca ttgccgcctg cgccacctca cgcagacgac
    19741 ggcggttaac aaacagaccg tggtgatagt gattcacggc gatattctcc cagacactca
    19801 ggttaggaaa taaagagagg tcctgataaa tcacctgaat cccctgcgcc accgaagcgg
    19861 cgggtgtcag ctttgaccag tttttcccac caagcgtaat ttccgctccc tcatccgggc
    19921 gataaacacc ggacataatt ttaatcaacg tcgatttacc acagccgttt tgcccggcaa
    19981 ggcagtgcac ttcacccgac ataaagtcga gagaaacatc gcgcagcgct cgcgtggcat
    20041 gaaacgtttt gttgatatga cgaagggaaa ggaaagtttc cataacatcc cctggcaata
    20101 agaatcgggg ctttcgcccc gaagaaatgg attaataaag tgagtcgata ttgtctttgt
    20161 tgaccagtaa taccttatgg aaacggataa ttcgcttatc catatctacg tcggccttac
    20221 ccagattctg catttctaat ccaggtttga tctcttcacc cttcagcaac gtgctggcga
    20281 cggctgcgag tgcgtaacct gcagaggccg gatcgtaagt aatcccttcg gtgatatcac
    20341 cacttttaat cagtgatgcc gcctgtgaag ggatcatcat gccatagact gcgactttat
    20401 ttttcgcccg tttctctttc accgcacgtc ccgcgccaat cggaccgttt gaaccaaagg
    20461 agacaaccgc tttcaagtca ggataggttt tcatcaggtc cagtgtggta cgacgtgagt
    20521 catccacact ctcggcaacc ggcatgcggc gggtaacttc atgcatatcc gggtaatgct
    20581 ctttctggta tttcaccagc aagtcagccc ataagttatg ctgcggcacg gtcaaactac
    20641 ccacgtaaat cacatagccg cccttgcccc ccatgcgttt cgccatatgc tcaacatatt
    20701 cagcggcaaa tttttcgtta tcaatgattt caatatccca gttagcactt ggctgaccgg
    20761 gggattcgtt ggtcagaacc acaattccgg catctcgcgc ttttttgaat accggttcca
    20821 gcacgttggc atcgtttggc acgatagtaa ttgcattaac cttacgggcg attaaatcct
    20881 caataatttt aacttgttgc ggagcatcag tacttgaagg ccccacctgt gaggcattaa
    20941 caccaaaggc tttacccgcc tcaaccacac cttcgcccat gcgattaaac cacggcatac
    21001 catcgacttt agaaatattc accacgactt tttccgctgc ctggagcggc gcagaaatta
    21061 gcgcagcgcc taataacagc gaagacacca tattgataac aaaacgttta ttcatcattt
    21121 cagcctcatt gcagggtagt caggaagtgt tcgcccaaat tagcgcgctg gagagaataa
    21181 gaattttcga atatttttat gcagaataaa cagccctatt tcaggcagct attaaaaata
    21241 tggttataaa tatggacaaa acgggcagca acaataagtg tgacattcat cacgtaaaaa
    21301 aatagcaaaa taaacaaaat tagcaatatt ttatgtgtgt gagtttattt cgcgtagata
    21361 aatagttatc tacacaacgt caataagggc atattcttca aatataacgt ccatttctgg
    21421 tgaatagctt ccttctgcct ggaaaaaacg acggtgttca ttacgccaat attcaagact
    21481 gaggtcacct tcaccttcct tacgggcaag ctccacggtg acatcagaaa agcgcagtaa
    21541 atgcaggcca atcactctga taacacatac aggctcacca cggctatttt ctacaatctt
    21601 atactcacca atcatcggaa atgcgttgtc ttcaatacat cccgccagcg aaccacagga
    21661 agctgtttta acaccagtcg caatcaacgt tgcgaggtga tcggccaacg ctgctgaatc
    21721 gccaaaagta caaaaggaag catggggata tttttgtgag aatttttctt ttatggtcat
    21781 ggcaatattt ttgcgattac ggaaaaacaa atcacatctt aattactgtt ggtaaccatc
    21841 agcaagagta tcattggttt ttcgatactg aatctttttt atccgaagca ggtcacattt
    21901 atggcctgat aaataccttt gttaaaactc acttttgtgt atttctctca aataatcccc
    21961 tggaaattat gccgcgagat tataaagttc aggatcattc attagcaaac cattcccgcc
    22021 ccactccctg gggcttaaat ctcttacaat tcaccgttgc aggcgtttca aatgcgcatc
    22081 aacctgccac actcccccac accagttgcc ctttatggaa cgttgcggtt ctcggcgaga
    22141 tacgcgccac cgcctcggcg gaacaggagg cgtccaccag cacaaagctg gcatcatcct
    22201 gcgcttttgg ccatacacgc tcgccttttt cattcagcgg caatacatcg ccagtggcta
    22261 gaaacagcga acgggagagg ttctgttcgt taggacgaat atagagctgc gcgtagagat
    22321 tggctttttc cagcatgtcg cccagaccat aaggcgacca gtggtcgata acgctgtcag
    22381 tgccagtcat cacttttacg cctttgtcgt gcaactgttt gagcggcata tgcagcgtgc
    22441 caatcggcac cgtcgaggcg atagaaattt gctgcgccgc catccgattc gccagcgcat
    22501 cgacctgttg ttcgttgagc gtagccagcg caaaggcgtg gctgatggtc agcttacctt
    22561 tcagttgcgg cgttttctct accgtttcaa ccatataatt gatggctgtc acgcccgacg
    22621 gagtggtttc gtgcaggtga atatcgacac ctttgtcgta gtccagcgca atctgaaaca
    22681 tggtgtcgag ggatttttcc atcgcgccat cgacactggt cgggtccagc ccgccgacgt
    22741 aatgcgcacc cgcctgcatc gcttcgcgca ttaagggttc cgatttcgac agcagcaaac
    22801 cgtgctgcgg gaaggcgacg atttcacact cgaagcccgc ctgacgtcgc gccagcaccg
    22861 cctgcaaatt ctgcagattt ttcaggccgg aaaccggttc gatattgcag tggctgcggg
    22921 caatggtggt gcctttcgac tgcaatagat cgatcagttt ttctgcccgt tcctgcgtgt
    22981 acggttgcag ttccggcagc attttctgct caagcttgat catgtcctgg atagtggtgc
    23041 ctgccggacg attgagcgag cgccacggcc cgccgtagaa ggttttgtcg agatgaatat
    23101 gcatgtcgcg agtggtgggt agcatcaact taccgccagc gtcatagtgc ggcagcgtgg
    23161 cgtccggatg ctgcttgttc tcgcgcaggg cgacaatttt gccgtcctga atctccacgg
    23221 tctggcgcgc cgtgcgggtc tgcaccgcca caccgttttc gtagtcaaaa ccggtttcca
    23281 gcagcacgtt atcgagatag taatgcgggt cagtgatttt catggtgttg ttcgcttcgc
    23341 agtttagggt gccagctacc gccgcatggg cgagcggcac agaggtacca aacagcgcgg
    23401 cggcggtgac catcttaccg ctctggctca gaaattcacg gcggctattg ctttctttca
    23461 tcctacgtcc ttacgtttta ataatatggg tgcccgtttc accgcgcagc gccgcaggca
    23521 ggcattccgg cgtggtgata atcacttctt tgccgccctg ctccaggaac gtcaggctgg
    23581 cgatgatttt tggcaacatg ctgccgggcg gaaaatgccc ttcctgcata tagcgagtca
    23641 tggtggcaat atccacccga tccagcgcct gctgctgcgg tttaccaaag tggatacaga
    23701 ctttttcgac gccggtggtg atcaccagaa tgtcggcgtg aatttcacgg gccagcagcg
    23761 cggtagagag atctttgtca ataaccgcgt ccacgctttg gtaatccccc gcttcagtac
    23821 gcactaccgg aattccaccg ccgcccgcgc caattacgac aaaaccttgt tggatcagcg
    23881 ccttaatggc gggtgcttcg acaatacgtt tcggttccgg cgaggcgacc acgcggcgat
    23941 agccccgtcc ggcatcttca acaaaacacc agtcagggtt tgccttttgt agtttgtcac
    24001 gctggctttc actgaagaat gcaccaatgg gcttggtggg atgggcaaaa cctggatcgt
    24061 ttttatccac ttccacctga gttaccacgg tgacggcttt tttctcgccg tgacgcgcca
    24121 gccggttatt cagcgcctgt tggatcagat agccgatgcc gccctgcgta tctgccacac
    24181 agttcgccag cggcgttaag ggcagccctt cgcgctcgtg ggcaatctcc gcacggcgta
    24241 aatccagccc gacctgcggc ccgttaccgt gggtcagcac aatgtcgtaa tcggaagcca
    24301 gcatttccag caccgtatcg gccacggctt tcaccgcctc cgcctgatgc tcaatcgact
    24361 ggctggcgtt atctttgata atgctgttgc caccaatggc aacgaccaca agctctttca
    24421 tgttgttatg tccgctacag agggtgatta ggttatgacc aacgccagac tgcctgatgc
    24481 gctacgctta tcaggcctac aattctcggg ccatccgtag gccggataag gcgttcacac
    24541 cgcatccggc ataaacatgt aagtcagacg ccgtaacgtt cgcaccagcc gagaatggct
    24601 ttctcgaagc aggcgaatgg tggatgcaca atgcccgcgc caatcatgcc gacgcccgca
    24661 tctttatggg cgatggcggt gttgatcacc ggcagaatgc cgctgctacc cacgcgggtg
    24721 atgtcgattg ccgacggcac gcccataaaa ccgagcagcg gaatggtgac gttggggttt
    24781 tcgccaaggg tgatttcgcg catctgacgg gagaaatcga tagcctcttc caccgtgccg
    24841 cccaccagtg cgacgattgc aggcgcggtc gccatggcaa acccgccaat accgtaggtt
    24901 tctgtgatgg cgctgtcgcc gatatccagc cccgaatctt ccggcttata accagcaaac
    24961 atcgggccga tgacctgctg cgctgggccg gtaaaccact gccccggcag gccgctgacc
    25021 cgcaggccga actcgacgcc gttacgcgcc atggtggtga ccacggtgct gtattcgatg
    25081 ccgtgcgccg catccatcgc cgctttacac atcgccatcc acgtcgggcc ggagaagtag
    25141 tcgctgctgg cgacaaactc gaacacttcg cgctgttgct cgacggaata gcctgcctga
    25201 atgatccccg gcgtcagtgc ctgaatcagc agcgtcgtcc cggcgttatt gcggttatgg
    25261 cactcatcgc ccatatgcag cgcctgcgcc agcattaagc gcagatcgat ctcaccgata
    25321 attttcatcg cgtcgcgcag cattggcccc tgcacatcgc gcatccagtt gaggcggtcg
    25381 atcacgctct gatcgttggc acccatacgc aaaatcttcg ccatctgctc gctcatattg
    25441 gtataagcga tgttgccgta ggttttgttt ttcacgatgt gcataaacat cgaggccgag
    25501 gtaacgcccg ccatcgatcc cacgcagtcg tgttcgtggc acggcgagaa ggtgatctcc
    25561 ccggaggccg ccagttcagt cgcctcatcg agatctttcg ccagcccttc gaacaccaac
    25621 gccccggtga ccgcgccttt catcgcgccg cacatttttt cccaggtgat cggcggcccg
    25681 gcgtgaagaa tggttttcgg cgtcatgccc ggcaccacgt taatcgcctg atcaaaacca
    25741 atcagcaccg gatgcgactg gataatgcgc tccagcgcct gctggttagc ggcggcgatt
    25801 ttgtctgcca gcggcgaatc ggcaatgtta tccagcgcct gcaccacctg catattgccc
    25861 tgccccggcg gcgtccagtc gagttgagtc acttctacat gctgcttttt caggtcatcg
    25921 ctaaacatgg cgataccgac gttaataacg ttcaacggtt ggctaaacag tgactggctc
    25981 atcaggcttc ctcccctttg cagataaatt cacgcgccag caatccggta ttggtgctgc
    26041 tgctcgccag aatcactccg gcatcaagca gcatctggct ttgttgttct aatgatggcg
    26101 tatcgagatc ggtgcccagc acataggcga gaatgatcaa ctcgcgtcct tcggcggcgg
    26161 cgatcgcttt cgcttctttg atcgcctcga tggtggagcc gaccggatct tcatgcgatc
    26221 caaatccgag cacaaaatcc atcacgatca ccgccacttc tggatcgcgc gcctcttcga
    26281 tcaagcgact gatgcggttg gtggggtcaa tcattggatg cggcttgccg ttggtgaagt
    26341 cgtcatcgcc aaagtcgagg aaggtgtgtt tgatgctacg gttgatatct tgcaggcgga
    26401 attccggatc gggctgaatg ttgctgtaga catcgccgtg tttttccatc accgcgaaca
    26461 tggtttcgtc acacagcgtg ccgccacaga acaggccacg aatgtacttc tgctgcggtt
    26521 gcaggcgagc acgtacgtca gcaatcaacg gctggttcag cgtatgcagg tcgagatgtt
    26581 cctgtttcac gccggagagc atcaccgctt tcaatgccgc ctctttgctg ccgcgggcaa
    26641 actgtagccc ctgctcatcc actggcgttt cgccacgacc gaggaaacag accaccaccg
    26701 gcttgcggca ggcgcgcgcg cgttccagca ctttgcgggc caccgcaggc gcgggcggtt
    26761 tggagataag cacaatgatt tcagtttgcg gatcgttttc cagcatgccg atggcgtcga
    26821 gcatcatcag gccgccgatt ttctcgctca ggtcgcgccc gccggtgcca atcagttgcg
    26881 aaacgccgcc gccaaattca tgaatgcgga cgctcagctc ctggctgccg gtgccggatg
    26941 cgccaacaat accgatgttg ccgcgacgca cggcgttacc aaaacagagc gccgcgccgt
    27001 taataatcgc cgtgccacag tctggcccca tcatcagcag ccctttttcg tgggccagtt
    27061 gcttgagcgc caattcatct tcaactgaga cgttatcgga aaacagcatc acgttgagat
    27121 cgttttgcag cgcctgacgc gcttcgcgag cggcaaacag accgttgacc gaaatcaccg
    27181 ccaggttact ttccgggata tgctttttgg cgctggcaat agtggcgtaa cgcgcctcat
    27241 gcgagccgct ttgcgctttg gtgttgaaca gttcttcaat ctccaccagc aactgttcgt
    27301 tgtccgcgcc cgctttgccg ttgatgacaa tcatcaggtc gccgtttttc gcctgttcca
    27361 gctccggcgt cagcagtcct aaatttttca gtacaccttt gttcatttcg gtcgccatcg
    27421 ccacaaatgc ctgctcgacg ccgtcgagtt tatttgcacg cgtggagata gacatcagcg
    27481 agacagaatc aaaataggta ttcggtttaa tgactatttt aactgacata attccctccg
    27541 taggtttggc tcagcgcaca accatcggcc atgccataca gcatgtcgca gccggaactg
    27601 gagccaatat gtttaatttt ttctattgcc tgagttgcgt tcccagggat gtcataaata
    27661 atgttgtgaa taaaacgatg aatattttcc cggcaacgtt gttgtaatgc ggcttccagc
    27721 gttatagcac ttaataatgt ggtgttattt ctgccgcgct gcataccgag ataaaattcc
    27781 tctttgtatt tttccgacgg atgcccggga ataaataaaa taagcgccag acctgttaaa
    27841 taatcgtcgg ctgatggcgt aaggccaatc cctaacccaa tcatactggc gaccgctgcg
    27901 gcgatatttt gtttttcgtt tactgcctga ataactgccc gtcgtttaat atgtagttct
    27961 ttatttaacg cctgataaaa cggattatcg ccgcgataat taaacagggt ttcattttcc
    28021 cccaggtgct gatgaataat gtcgtgccac tgttgccagg cgataaggtg aaaattcaca
    28081 gcggtcattt gcaaggttgg ggacagccaa cgccgacaac gactcatctc tatatgaaga
    28141 tgttgaccaa ccgtaatacc ttgatcgtca aactgaacct tatcaccatg ccggaacaga
    28201 tcatcaaagt gagtgagtgc caaccgacaa ctgtttggcg cattgtcgta ctcttcacaa
    28261 agcagcgtca gcaactgctg gcgagccggg agtgcgatat ttaccgctct ggcaaagacc
    28321 tgttcaacct gtccttgccc ccgctgcgcc agaaaatccg catccgcagt tagcgcccac
    28381 acacaccgtc tttctggtgt gttgatagca agcctcccgg tttatgcacc tgcggcaatc
    28441 agtaactgcg caatctcttc aaagccccgt tcccgcgcca gttccagtgg cgttttgcca
    28501 tatttatcgg tcagatgcgg gctggcaccg tgttccagca ataactgcac aatcgcctgc
    28561 tgtttaatac caccatcatt aagcacaatc gcttccagta gcggcgtcca gcctacatgg
    28621 ttggtctggt taacgttaat ctccgtatgc gccaaaagct cttttacaat acttaaatgg
    28681 cctttttcac aggcaggcgt caggccgaca ccgccaaaac gggttacgca attaagatcg
    28741 ggtttagccg gtaaaataat tcgtagtagc gttaaatcat cgttcagaca gctaattaaa
    28801 aaaggattta aacaggtatg atcttgctta ttaatatccg ctccggcatc aattaatgcc
    28861 tgaacgcaag catattgctg atataaactt gccagcgtaa ttgccgtttt cccctgacga
    28921 tcgcaggtat taatatcgac acccagcgca aggcaggttt ttactttatc gatatcaccc
    28981 tgttgtgcag ccaataaata gtcggcaggt agatttttta tactcattca cattcccctg
    29041 gtttttgtgg ctgttttcga tgaattgaga atagcaacga taatctgata aaagaacccg
    29101 cttaaattta attcgctgta tttcgccttt ttgttgaatg atgttcaaca atcttttcgc
    29161 agtgtgcggt agtgcaatat ttgtttcttt gacccgatac cctttcactc aatcaggttt
    29221 tatttctcta tagtctgcac atgtaagagt agctttcgtc catgtctaac cagaggggaa
    29281 caagatgaac ggactcacgg caacgggtgt caccgtcggt atttgtgcag ggctttggca
    29341 gttagtgtct tcacatgtcg ggctctctca gggatgggaa ttattaggaa ccattggctt
    29401 cgtcgccttt tgcagttttt atgccgcagg cggcagtaaa tcggggttta tcagaagtct
    29461 ggcggttaat tattccggga tggtgtgggc atttttcgcc gcgctggctg ccggttggct
    29521 ggcctctgta agtggactgt ccgcgttctg ggcgagcgta attactaccg tacccttctc
    29581 ggcggtagtg gtctggcaag ggcgtttctg gttactgtcc tttattcccg gtggctttct
    29641 cggcatgacg ctgttttttg ccagcgggat gaactggacg gtgacgttac tgggttttct
    29701 ggcgggtaac tgcgtgggga taatttcaga atatggtgga caaaaattaa gcgaagcgac
    29761 gactaaacgc gacgggtatt aaaagttatt caaaaatgat tatctatctt taaccgatac
    29821 ctctacagtc atagctggag aaattcatag tagataatgg cgagaatggt ttaatgaact
    29881 caatttttac cgaagagaat ctgttggcat ttactaccgc tgcacgtttt ggcagcttca
    29941 gtaaagccgc agaagagttg ggtttaacca cttccgccat tagctacacc atcaagcgta
    30001 tggagacggg gctggatgtg gtgctgttca ctcgcagtac ccgcagcatt gagttaacgg
    30061 agtccgggcg ttattttttc cgcaaagcta ccgacctgct gaatgatttt tatgccatca
    30121 aacgcagtat cgatactatt tcgcagggca tagaagcgcg tgtgcgcatt tgtattaatc
    30181 agctgcttta tacgcccaaa cacaccgcac gcttgttaca ggtgctgaaa aagcagtttc
    30241 ctacctgcca gattaccgtc accaccgaag tgtataacgg cgtctgggat gcgattatta
    30301 ataatcaggc caatatcgcc atcggcgcac ccgatacact actggacggc ggcggtattg
    30361 attacactga aattggcgcg attcgctggg cttttgctat cgcgccggat cacccgttag
    30421 cctttgtgcc ggaacccatc gccgaaagcc aactgcgtct ctaccctaat attatggtgg
    30481 aggacaccgc gcatacgatt aataaaaaag tgggctggtt gctgcacggg caggagtcga
    30541 ttctggtgcc agatttcaac accaaatgtc agtgccagat tttaggtgaa ggaattggtt
    30601 ttttgccgga ttacatggtc cgtgaggcga tgacgcaatc cctgctggtt acccgacaaa
    30661 tccataatcc gcgccaggat tcgcgcatgt tactggcgac tcagcattct gctacgggtc
    30721 aggtgacgca gtggattaaa aaacaatttg cgccaaatgg catattaacg gggatctatc
    30781 aggatttact gcatcgggag aattaatcgc tgccaacctc ccgatgcgct gtgaatagcg
    30841 gtttacatca accacctgct ttcattaccc attcagagat aaatttatta cccggtactg
    30901 gcggagagta caaatagccc tgcaaaaagt gaacgccttt accgatcatt aaatccgcct
    30961 gctcctgggt ttctacccct tccgccacga tactcagacc aggcttacgc gctagttcga
    31021 caatattgtc cacaatatga ccggagattt cgtcgacact cgccatttgc acaaatgact
    31081 tatcgatctt aataaaatcg accgggaacg cctgcaagta acgataggtc gcataacccg
    31141 taccaaagtc atccagcgca aaggtaatgt tgtgctgatg aaggctgtca aatatcgctc
    31201 tggcttctgg cgttaccgga atagggttac gttccgttag ctcgagaacc agcttgattt
    31261 tatcgttacc taatttctta accaggttca gacactcttt ttcaaatccc gctgccaaaa
    31321 aacaacccgc cgagacgttg atgccaatat ggaaattgtc cggcagcaaa tgttttaccg
    31381 gcatcagaat atccgcagtc tgtttcatca gttggcgggt cattatgaca ataagaccgg
    31441 atgactccgc cagaggaata aactgatccg gtgggataat tcccgtttgt ggatgttccc
    31501 agcggacaag tacctcacag cccgtcagta ccccagtctg cgcgcagaaa accggttgga
    31561 tccacggttt gaattcgtgg ttttccaacg ccagactgat ggcttcaggc gtgacgatat
    31621 ggtgacaacg gttatcaggg atataaaaat tatttttatt tcccgtgcca ctgatgactt
    31681 gcggatgata ttgaaaaaag atatcccgcc agaaggtaat atcactctgg atgcctagtt
    31741 tttcatacag ctgcttcttt tgatgtgaaa ttgtctttgc actgcgattt ctgtactgtg
    31801 agatttctgt aaccgacatt ccctccatta gcagcttgag taaaagccgt tctttttcgg
    31861 acacgatacc aggcaacgtc aaattcaccg ttgccccgaa ctcttccaaa aaaacactca
    31921 aatcatgcga agccattgaa cacaccttga ttaaatcttg tgcgccagct aaatctgcga
    31981 gtcttcactc tgactgataa agctggctgc aagaaaatga cacagagagc cgaattatct
    32041 cccgttcggc tctctctcct tttactcatt attcagatca gaattgccac ttaatgccaa
    32101 ccattgcaga ggtgtcatta tatcccctat cggcaacctg aacgcccaca ttcccccaga
    32161 cattcaggtt ggcatttagc tgtccttcca ccccggtttt tatctcagca atatttcggg
    32221 ctccatcctg agtgacggac acgccatcca tactggtgct gaaatcctta ctgttatgca
    32281 gccagttcac ttctacaaac ggctggaact cgcgggattt accgtcatcc attttatggt
    32341 ggctcttcag ccaggttttt acgccaagtc gggtttgaac attgccatca ccgttgctat
    32401 gaacgagggt tccgttgctt tcgcggtgct tatcggcttt gactcccatc caggtaacct
    32461 gtgcttgcgg ctgaacatac cattcattac gcgttccctg gttgccatta aattcagcta
    32521 atttgtgttt gtatccagct tccagtgaag cggtaaatcc ttttgattta taggattcac
    32581 tttgtaagtc atcccctttc actgtgttat caaaccagct gtactgcgcc caactgtcga
    32641 gatacgcgcc attacgcgac tcgtcatcgg cataccaggt ggcatagagg ccggtgctat
    32701 atccgttcac actcgctttt gcacgataac cggttcgcga ggaaatggtt ttgctgtcgc
    32761 tgttgccata tcccgccatg accccaacat gccagctgtc gctgccgttt tggctccact
    32821 gcgcgacatc gcctcccagt tgcagaacat agcgattgct ttgggttttc agctggccgc
    32881 tgccatcacg ccatttatta tgaccacctt catggcgcat ccacatagtg gtttgtttct
    32941 gctcacccgt caccatgtcg gtatagtacg tattacccag acgctcatgc agacgcgtgg
    33001 tgaacattgt attcgctgct gcaaggttcg caatgtagct acccgcctcc ggtcgcaggt
    33061 cattatccac attcagatcc ggacccggcg taggtgtcgg gttagggttc ggctctggat
    33121 tcgggtttgg ctccggattc ggcatcgggt ctggctccgg ctgcagttca ggagaatcac
    33181 tgccgctggt cagataccag ttaccacgat ttgccccttg tccacgcgcg agagtgtagt
    33241 catacgcccc cgcaacgata cgaccagcct gaacaaattc gccctcagac ttaccgtcta
    33301 catggataag ttcgataccg ttaagggttt tcgcacctgt accgccagcg ttattcaccg
    33361 taacggcagt cgtgccgcta gtatcgccct cgacaaccaa tttatcggtt actgagtcat
    33421 cgccattcag taccgtattc attactagtt gaccgttatt accgtggtag ttccccttca
    33481 ccactaacac attgccagct tgctgtcctg acattccggt catcagcgta ccgttgttgg
    33541 tcagattgcc gcccagggtg taattcacag acgcagccgc tcttttttca gcgctattat
    33601 tcaacgccag ggtaccgttg ttctcaacgt ttgcgctacc caatccctgc gcatgactta
    33661 ccaggacatt agccgaatta gcaatgttca cattcgccgt caggttgctg ttatcgcccg
    33721 cgatgtccag cgtgccgccc tgaatattca gctctccgct ccccgttaac gaaccggttg
    33781 aagtgccccc gttggtaacc gtcagactgc cgccatttaa gctcagcagt gaatcggcgc
    33841 tgccattgag cgtgcccacg gtctggctgt gaccattcat atccagttca gtctctgccg
    33901 ccagacgcag ttcatgggta ttacccaaca cgttatcgtt attcaacaaa agcgtcccac
    33961 tgcgcagatc ggtaacaccg gtatagtcgt tgtctttgtt agaaagcgat acggtctgat
    34021 ccttctggct gctgaatgcc aggtcaccgc tgccggtaat ctttgcgctg agatcggcgg
    34081 cattctcgct tttaccgtta gagctcagca ccagagcatc gctatcggta gcgtgtaaat
    34141 caagctgggt caggccgtaa ccgatataca gaccgtcatt gtttacccca ctcgtcagac
    34201 gatagtcgta agtgccgacg gccgcttttt gcccattctg ctgaatatca cgttgggcac
    34261 tgctggagag gatttgccca tttttatcct gcaattgcag attgcccgca tcgcccagaa
    34321 cttccgcacc ttgcgcatca accaacttaa tggtgctgtt accatcatcg acttcagtga
    34381 gcgataacgc agagtcaata tcacgagata ctgagcttac aacgtcactg tcagaaacct
    34441 gaatcacacc ctcgccgcgc aaatccagcg ttttactaac gttgaccgtc ccttctgtca
    34501 tctgtgcacc tgctgtcagg gcaccaaaat caacggttcc gccggagaat cccaaagtgc
    34561 taatggagtg agcgccctta cccactgtta ccaggcttcc ctgacccgca gtgatcccgc
    34621 ctctctgcag aaactcagca ttcttttcca ggttaaatgt cgtgttctgg aacgccagtg
    34681 aaccctggaa ccagtctgta taagcaaact catcgaagga gaattcgtga ttccccatgt
    34741 ttatttcaac aatacctcta ccttgaagat cgttatcaaa tacccaatct ttattggcat
    34801 tgatttgcag tttgccatca acatccaggt ccgcacggcc gatgttcttg tcttcagaaa
    34861 taaccgcgac agcaccagaa gcgacatcaa tatatccgct gaatgcatag ttattgccgg
    34921 tcagtgccac tgtcccactg tcgatactta ttgtaccatt gccggaaatc ttattggcaa
    34981 aggtcatgct atcgtcagcc ttcaggtgta atttcccgcg gttgttgatc tctgctgtac
    35041 ctatattttt ggtgatccct aaagcgccat tttgctcaat tttgacttta ccactaaagg
    35101 cagagttatc accggttaag taagtcgtgc cattgagcgc attgatatag cccaggccct
    35161 cgctgccact gacaatatcc gcggaaatag tcgcgttttt atccgtgtgg tttaaggtga
    35221 ttttcttatc ggatgtcttt gcccacacaa actcaatttt ttcggcatca ataatccctg
    35281 cagccttcgc agcacttcct tcctgtgcac ctaacgctaa ctcagagttg gtgcttaaac
    35341 taattttcgg tgcggatatt ttacctccat ctgatacgac taatgttgta tcagaacgtt
    35401 ggtcgtaata acccaggcta atatgttcac gagcattaac ttcaccaccg ttattaaccg
    35461 acaggaactg attaccgcca tatccgccgt aaacgcccat aaagaaggtc cctgcattta
    35521 cctgactacc ttcgccgctt acattcccct gcgtataagt cagacgactg gtgcccttaa
    35581 catgaatttt gccgccattg gagacattaa aatagccttg atcatgtctc atgcctgagt
    35641 ttgactcata caaatcatca gcatataact cgctgccagc attggagaca ttcaggaacc
    35701 ccgtaccttt cagcaaaaag ttaagcgggc cattgattgt tacctttcct ccggtaatat
    35761 ccaacccacc accagaagcg agagtcagac gcggacggga agtatctgtc gtattgatta
    35821 ccagattggc attgtcagtc accgtaatac ggtaatcgat gctctggttc tctttgccaa
    35881 aatcaatagc tttatcctgg tttacgatga catcatcggc gaataccaga gatgaatata
    35941 aacttatcgc gctaattaaa acagcccggg acttttttcc ttttactctg ctccccagct
    36001 ccgagcagac gctccaacag ttacgggacc tgttccactt tagccgatat attttattca
    36061 tcacaatctc cagcaataca tccaaataaa accaagattg cataaacagc aacgcattaa
    36121 atgccattct ggttactgtt catcgcatat aaaaattcaa aaattttgca tagcattggc
    36181 aaccacatat ctgagtatgt agttcagagg gtaataaaat agcaagttat tacttaaaac
    36241 ttattttaag ttaacactct taact
//
LOCUS       DAZGCS010000045        36122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGCS010000045 DAZGCS010000000
VERSION     DAZGCS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.045"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..131
                     /gene="yecU"
                     /locus_tag="ACHK01_002289"
     CDS             <1..131
                     /gene="yecU"
                     /locus_tag="ACHK01_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO8097235.1"
                     /translation="ADITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            118..342
                     /gene="yecF"
                     /locus_tag="ACHK01_002290"
     CDS             118..342
                     /gene="yecF"
                     /locus_tag="ACHK01_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO8097236.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(409..1131)
                     /gene="sdiA"
                     /locus_tag="ACHK01_002291"
     CDS             complement(409..1131)
                     /gene="sdiA"
                     /locus_tag="ACHK01_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO8097237.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1361..2113)
                     /gene="tcyN"
                     /locus_tag="ACHK01_002292"
     CDS             complement(1361..2113)
                     /gene="tcyN"
                     /locus_tag="ACHK01_002292"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO8097238.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(2110..2778)
                     /gene="tcyL"
                     /locus_tag="ACHK01_002293"
     CDS             complement(2110..2778)
                     /gene="tcyL"
                     /locus_tag="ACHK01_002293"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO8097239.1"
                     /translation="MQESIQLVIDSLPFLLKGAEYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2793..3779)
                     /gene="dcyD"
                     /locus_tag="ACHK01_002294"
     CDS             complement(2793..3779)
                     /gene="dcyD"
                     /locus_tag="ACHK01_002294"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO8097240.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3884..4684)
                     /gene="tcyJ"
                     /locus_tag="ACHK01_002295"
     CDS             complement(3884..4684)
                     /gene="tcyJ"
                     /locus_tag="ACHK01_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8097241.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4772..5323)
                     /gene="fliZ"
                     /locus_tag="ACHK01_002296"
     CDS             complement(4772..5323)
                     /gene="fliZ"
                     /locus_tag="ACHK01_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO8097242.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTAREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5369..6088)
                     /gene="fliA"
                     /locus_tag="ACHK01_002297"
     CDS             complement(5369..6088)
                     /gene="fliA"
                     /locus_tag="ACHK01_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO8097243.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6253..7746)
                     /locus_tag="ACHK01_002298"
     CDS             complement(6253..7746)
                     /locus_tag="ACHK01_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO8097244.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            8001..9413
                     /gene="fliD"
                     /locus_tag="ACHK01_002299"
     CDS             8001..9413
                     /gene="fliD"
                     /locus_tag="ACHK01_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO8097245.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            9428..9838
                     /gene="fliS"
                     /locus_tag="ACHK01_002300"
     CDS             9428..9838
                     /gene="fliS"
                     /locus_tag="ACHK01_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO8097246.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9838..10203
                     /gene="fliT"
                     /locus_tag="ACHK01_002301"
     CDS             9838..10203
                     /gene="fliT"
                     /locus_tag="ACHK01_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO8097247.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            10281..11768
                     /gene="amyA"
                     /locus_tag="ACHK01_002302"
     CDS             10281..11768
                     /gene="amyA"
                     /locus_tag="ACHK01_002302"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8097248.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11802..12215)
                     /gene="yedD"
                     /locus_tag="ACHK01_002303"
     CDS             complement(11802..12215)
                     /gene="yedD"
                     /locus_tag="ACHK01_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO8097249.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12402..13607
                     /gene="yedE"
                     /locus_tag="ACHK01_002304"
     CDS             12402..13607
                     /gene="yedE"
                     /locus_tag="ACHK01_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO8097250.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            13604..13837
                     /gene="yedF"
                     /locus_tag="ACHK01_002305"
     CDS             13604..13837
                     /gene="yedF"
                     /locus_tag="ACHK01_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO8097251.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13946..14614
                     /gene="yedK"
                     /locus_tag="ACHK01_002306"
     CDS             13946..14614
                     /gene="yedK"
                     /locus_tag="ACHK01_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO8097252.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(15007..15321)
                     /gene="fliE"
                     /locus_tag="ACHK01_002307"
     CDS             complement(15007..15321)
                     /gene="fliE"
                     /locus_tag="ACHK01_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO8097253.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            15536..17194
                     /gene="fliF"
                     /locus_tag="ACHK01_002308"
     CDS             15536..17194
                     /gene="fliF"
                     /locus_tag="ACHK01_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO8097254.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMMQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            17187..18182
                     /gene="fliG"
                     /locus_tag="ACHK01_002309"
     CDS             17187..18182
                     /gene="fliG"
                     /locus_tag="ACHK01_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO8097255.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            18175..18861
                     /gene="fliH"
                     /locus_tag="ACHK01_002310"
     CDS             18175..18861
                     /gene="fliH"
                     /locus_tag="ACHK01_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO8097256.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            18861..20234
                     /gene="fliI"
                     /locus_tag="ACHK01_002311"
     CDS             18861..20234
                     /gene="fliI"
                     /locus_tag="ACHK01_002311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO8097257.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            20253..20696
                     /gene="fliJ"
                     /locus_tag="ACHK01_002312"
     CDS             20253..20696
                     /gene="fliJ"
                     /locus_tag="ACHK01_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO8097258.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            20693..21820
                     /gene="fliK"
                     /locus_tag="ACHK01_002313"
     CDS             20693..21820
                     /gene="fliK"
                     /locus_tag="ACHK01_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO8097259.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            21925..22389
                     /gene="fliL"
                     /locus_tag="ACHK01_002314"
     CDS             21925..22389
                     /gene="fliL"
                     /locus_tag="ACHK01_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO8097260.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            22394..23398
                     /gene="fliM"
                     /locus_tag="ACHK01_002315"
     CDS             22394..23398
                     /gene="fliM"
                     /locus_tag="ACHK01_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO8097261.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            23395..23808
                     /gene="fliN"
                     /locus_tag="ACHK01_002316"
     CDS             23395..23808
                     /gene="fliN"
                     /locus_tag="ACHK01_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO8097262.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            23811..24176
                     /gene="fliO"
                     /locus_tag="ACHK01_002317"
     CDS             23811..24176
                     /gene="fliO"
                     /locus_tag="ACHK01_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO8097263.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            24176..24913
                     /gene="fliP"
                     /locus_tag="ACHK01_002318"
     CDS             24176..24913
                     /gene="fliP"
                     /locus_tag="ACHK01_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO8097264.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPLKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            24923..25192
                     /gene="fliQ"
                     /locus_tag="ACHK01_002319"
     CDS             24923..25192
                     /gene="fliQ"
                     /locus_tag="ACHK01_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO8097265.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            25201..25986
                     /gene="fliR"
                     /locus_tag="ACHK01_002320"
     CDS             25201..25986
                     /gene="fliR"
                     /locus_tag="ACHK01_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO8097266.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            26276..26899
                     /gene="rcsA"
                     /locus_tag="ACHK01_002321"
     CDS             26276..26899
                     /gene="rcsA"
                     /locus_tag="ACHK01_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO8097267.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(26943..27131)
                     /gene="dsrB"
                     /locus_tag="ACHK01_002322"
     CDS             complement(26943..27131)
                     /gene="dsrB"
                     /locus_tag="ACHK01_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO8097268.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            27294..27521
                     /gene="yodD"
                     /locus_tag="ACHK01_002323"
     CDS             27294..27521
                     /gene="yodD"
                     /locus_tag="ACHK01_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO8097269.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            27819..28634
                     /gene="yedP"
                     /locus_tag="ACHK01_002324"
     CDS             27819..28634
                     /gene="yedP"
                     /locus_tag="ACHK01_002324"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO8097270.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(28631..30325)
                     /gene="dgcQ"
                     /locus_tag="ACHK01_002325"
     CDS             complement(28631..30325)
                     /gene="dgcQ"
                     /locus_tag="ACHK01_002325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO8097271.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPFNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(30496..30678)
                     /gene="yodC"
                     /locus_tag="ACHK01_002326"
     CDS             complement(30496..30678)
                     /gene="yodC"
                     /locus_tag="ACHK01_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO8097272.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(30757..31674)
                     /gene="yedI"
                     /locus_tag="ACHK01_002327"
     CDS             complement(30757..31674)
                     /gene="yedI"
                     /locus_tag="ACHK01_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO8097273.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            31847..32767
                     /gene="yedA"
                     /locus_tag="ACHK01_002328"
     CDS             31847..32767
                     /gene="yedA"
                     /locus_tag="ACHK01_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO8097274.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(32756..33226)
                     /gene="vsr"
                     /locus_tag="ACHK01_002329"
     CDS             complement(32756..33226)
                     /gene="vsr"
                     /locus_tag="ACHK01_002329"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO8097275.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(33207..34625)
                     /gene="dcm"
                     /locus_tag="ACHK01_002330"
     CDS             complement(33207..34625)
                     /gene="dcm"
                     /locus_tag="ACHK01_002330"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO8097276.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDEADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(34692..35387)
                     /gene="yedJ"
                     /locus_tag="ACHK01_002331"
     CDS             complement(34692..35387)
                     /gene="yedJ"
                     /locus_tag="ACHK01_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO8097277.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQGAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(35427..35708)
                     /gene="drpB"
                     /locus_tag="ACHK01_002332"
     CDS             complement(35427..35708)
                     /gene="drpB"
                     /locus_tag="ACHK01_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO8097278.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         8880 a         9362 c         9078 g         8802 t
ORIGIN      
        1 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
       61 ccgccgcctt aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg
      121 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
      181 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
      241 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
      301 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
      361 cgtgcctttc agccggtttt tgcatctggc acgcagaaag aaaagcgatc aaattaagcc
      421 agtagcggcc gcgtaacagg ccacctgggt cttatttggt gcattgattt ttttctgcat
      481 gtttttctga tggaaattga ccgtattctc agagattgac aaaatcatcg ctatctctgc
      541 tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat
      601 ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac
      661 cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca
      721 acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc
      781 gcggcgtaaa ccatgtgcgc gcgcggcttc ccataacggc tgtgcttcgc tgaataagtc
      841 atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcaccg gatcaattgc
      901 gagaaagttt tttgcctgat aataactaac ccacgcctca gggtaattgg tgtagaaagc
      961 cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc
     1021 cagctgctga gcctgaagct ctatttcatg gtagacctct tctgcggcct ccatcctctg
     1081 aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg
     1141 caacgcccct gagagtgagt atcatttata atgataattc aaactatcac ataaataaga
     1201 tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct
     1261 tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc
     1321 ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc agaaacttct
     1381 cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct
     1441 gctcgactat ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc
     1501 tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt
     1561 tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca
     1621 cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca
     1681 aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag
     1741 cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt
     1801 tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct
     1861 gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga
     1921 tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctgcgtagca
     1981 acgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct
     2041 cgaggtcgat accgtgcagc accgtctgac cgtgaaattt tttcaccagg ttcttaactt
     2101 caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg
     2161 caacgtcgat aatactgtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt
     2221 gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg
     2281 gattgtcgcg gccagcgacg tatcttttac cagactgata aagctgttcg acagcggcgg
     2341 caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat
     2401 accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag
     2461 cgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg
     2521 atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag
     2581 cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg
     2641 cgacaggcgc atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat
     2701 actgagttgc agggtatact cagccccttt caacaggaac ggcaaggaat caataaccag
     2761 ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc
     2821 gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga
     2881 ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg
     2941 ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag
     3001 ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct
     3061 ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac
     3121 acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccaatccg
     3181 gcgtgagttc cggcactgcc cgatgcgacc accaccgacg aaatattaac cgccccttca
     3241 cactgttgcg cgatttccag cgcactctcc acataaccca gcgcgcccag agcattagaa
     3301 ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct
     3361 tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga
     3421 tccagcaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt
     3481 tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca
     3541 tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct
     3601 gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg tgtgacgtca
     3661 tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc
     3721 ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata
     3781 acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata
     3841 aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag
     3901 caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg
     3961 catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct
     4021 gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca
     4081 gcgccgccag acgatcaacg aggatcgcat cgatacggcc tacgcgcaga tcctgatatt
     4141 tggtcgggtc atcatcatag gtacgcacat cgacgccctg aacattctgc cgcagccact
     4201 cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt
     4261 taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg
     4321 tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat
     4381 caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg
     4441 cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt
     4501 tgccgtcatc tccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg
     4561 tgccgcgctc tttaacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc
     4621 ccgcaaccag cgctacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt
     4681 tcatatttac cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca
     4741 caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg
     4801 aggatttctg cacaagtccg gtacacgttt gacgctgata gtgttgatac ttccgtaatg
     4861 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
     4921 aaccgtcctg cagcggatcg agggaaatat tttgctcgtg tagatgattc cccaaacggc
     4981 gcagacgaac gacatattca cgcgccgtac cagggctcat ttcagtttgc tcaaaaagaa
     5041 attgcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
     5101 agaatcggca caatgccgcc catgattttt gttccacttg ccagccattt tcatcaagca
     5161 gcatgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga acgagggtaa
     5221 tgcggtcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa
     5281 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
     5341 caaaattaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
     5401 taatggcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
     5461 tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg
     5521 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta
     5581 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc
     5641 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcaacatt tggcgataat
     5701 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
     5761 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
     5821 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt
     5881 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacggcatta agtaacccaa
     5941 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
     6001 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
     6061 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
     6121 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
     6181 atttgggggt taccggtaaa ttccatgcag aaaaaacccc gccggtggcg gggtttgagc
     6241 gataagtgta aattaacgca gcagagacag tacgttctgc gtggtctggt tagcctgtgc
     6301 cagaacagaa gtacctgctt gttgcaggat ctgcgcacga gacatgttag acacttcggt
     6361 cgcgtagtca gcatcttcga tacggctacg ggcagaagac aggttgttta cggtgttgcc
     6421 aaggttggtg atggcagagt cgaaacgatt ttgaattgca cctaggttag aacgcaacga
     6481 gtcaacctga gacagtgctt tatcgatagc agccaaagga tcttctgttt tctgcgtaga
     6541 aattgctgca atagaatcag ttttcaggct cttaccttgt aaatcactaa cactgtaagt
     6601 tttgccatcg gttgcagtta caacttcggt tttgccatcg cttccaccca gttcaacttt
     6661 aacattgtcg tgtttgacac cgtcagcatc agtgtaagac attgttttaa cggaaactgc
     6721 tcctgttttc tgatcaacat tcgctgcata ctgtttaccg tcatcaccaa tcaacgcaaa
     6781 tgaaccagta tctttgccat tggcgtcttc taacttaaag agttttgcac ctgtgataga
     6841 agcggttgcg gtatcagtac ccaactgaac atcaatagcc cccttccctt tcaccgttgt
     6901 gacttccttt aaattaccag cttgaggctg ggtacccgct agagccccca gcttcagctt
     6961 acctgcatca ggagatgtcg gatcatcctc cacagtaact tcataataac cattttcagc
     7021 gggtttatct gaaccggcaa ctttaacgta caacttaccg ttagcatcag acataatatt
     7081 accgtcacgt tggcccggag tcgtagtgcc tttaactgta gctgtaatat ctgcatcaag
     7141 tgtattagcg tcagcaagct taactgtcga tccaaaatag gttgtttcag ccgttgcggc
     7201 ttttaattta gtaggatctt taataccaaa accagataat cccagtgtgt cggaattaat
     7261 ttcctgcagt ttaatagtaa ttgtttcgcc gtcgtttgca ccaacctgaa tagtcatatc
     7321 ctgatcagaa gccagcactt taacgccgtt aaactgagtc tgaccagaaa cacggtcaat
     7381 ttcactcaga cgctgctgga tttcggactg gatggaggtc aggtcactgt cagagttagt
     7441 accgttagtt gcctgaacag aaagttcacg aatacgctgc aggttgttgt taatttcatt
     7501 cagcgcacct tcagtggtct gcgcaacaga aataccatca ttcgcgttac gggaagcctg
     7561 agtcagacct ttaatatttg ctgtaaaacg gttagcaatc gcctgacctg ctgcgtcatc
     7621 tttagcactg ttaatacgca ggccagaaga gagacgttca atggcggagc tcagagaaga
     7681 ctgagatttg ttcaggttat tctgggtcaa cagcgacagg ctgtttgtat taatgacttg
     7741 tgccatgatc ttttccttat caattacaac ttgatgttat tgggctgttg cccacggttt
     7801 ctcaccgtac cccttgtatc ggcacctgaa tttcgaactt tagaaaattt tagtttctct
     7861 cctgtttttt cttaacggct gaaatatccg taatatacaa ctctactctg cgcatcatct
     7921 tcgtgaatca actattaaac tttgcgcaca aattgccgat aacccgctta tctactgttt
     7981 gcaatcaaaa ggaattaggt atggcttcat ttacatctct tggcgttggt tcgaatctgc
     8041 cgctggatac attgctaaac aacctgacca tcgctgaaaa aaaacgctta aacccaatta
     8101 ctcagcaaca gagtgacaat accgcacgtc tgacagccta tggcacatta aaaagtgcat
     8161 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     8221 ccgttaccag tagcaatgaa gaccttaaag tttccaccga agcaggtgct gctcccggta
     8281 tctataccat cagcgtaacc caactggcgc aggcgcaatc tttacgaact gattccccga
     8341 caatcattgc atcaacaaaa gatgcactgg gtgatgagtc atcagataca cgcacaataa
     8401 aaattaccca agacggtcgt aaagagccgc tggaaatcaa gctcaataaa gatcagacgt
     8461 cattggatga gatcagcaaa gccattaacg atgccgatag tggaatttcc gctagtatcg
     8521 ttaaagtcaa agatggtaac tatcaattgg tattgaccgc cagtgaaggt ctggccaata
     8581 aaatgaccat atctgttgag ggtgacagta aattaaatga tctgctggct tatgacagca
     8641 agaccaacac cgggaatatg aaggaactgg ttaacgcgca aaatgcacag ctaaacgtta
     8701 acggtatcga tattgaacgt tcaagtaaca aaattaccga tgctcctcag ggagtgactc
     8761 tcgatttgac caaaaaagtc actgacgtcc gggtcacggt gacaaaaagc aacgataaag
     8821 caaccgaagc aattaaaggc tgggtggatt catataactc cctcattgac acctttaaca
     8881 ccctgacaaa atataaagaa gtagacccag gtgctgaagc acaagataaa aataatggtg
     8941 cacttctcgg agacagcgtt gtacgtacaa ttcaaagcgg catccgcgca caatttgcaa
     9001 acggtgccag cgacggtgcc ttcaaaacat taaacgagat cgggattaag caggacggca
     9061 cgacgggtaa gctgaaaatt gatgatgata aactcaaaaa agtcctgaat gaaaatacag
     9121 catccgtacg tgaattgctg gtaggcgatg gtaaagaaac aggaattacc accaaaattg
     9181 ccaccgaggt aaaaggttac cttgccgatg atggcattat tgatagcgca caggacagta
     9241 ttaacgccac actgaaaaaa ctgaccaaac agtatctatc cgtcagtgcc agcattgatg
     9301 acaccgtggc gcgttacact gcacagttta cgcaattaga taccatgatg agcaaactga
     9361 ataacaccag tacctatctc agccaacaat ttactgcaat gagtaattcc tgatcacgga
     9421 ggtcagcatg tataccgcga gcggctcaaa agcctatgca caaattggcg tcgaaagcgc
     9481 cgtaatgagc gccagccagc agcagctggt caccatgcta tttgatggag tgctgagcgc
     9541 actggttaga gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt
     9601 gtcaaaagcg atcaacatca ttgagaacgg acttcgggtg agtcttgatg aagagagcaa
     9661 agacgaatta acccaaaact tgattgctct ttatagctat atggtcaggc gcttgctgca
     9721 agccaattta cgcaacgatg tctccgcagt cgaagaagtg gaagcattaa tgcgtaatat
     9781 tgccgatgcc tggaaagagt cgttactctc cccttctttg attcaggacc cagtctgatg
     9841 aaccatgcac cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg
     9901 ttacgcctgg caacggaaga acaatgggac gaactcatcg ccagcgaaat ggcgtatgtg
     9961 aatgcggtgc aggagatagc acacctgact gaagaggttg ccccgtccac cacgatgcag
    10021 gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta
    10081 ttacagattc gtatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg
    10141 gtgttaagtg cctatggcga tcagggcggc tttgtgctgg ctccgcaaga taacttctcg
    10201 tgaatcttaa cgagctaatg gttcgcgaat aatccgatta cggctacgct tctaatgttc
    10261 cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt
    10321 acccggaagg cggtaagctc tggcctgaac tggccgagcg cgccgacggt tttaatgata
    10381 ttggtatcaa tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtattcgg
    10441 tcggctacga ctcctatgat ttatttgatt taggcgagtt tgatcagaaa ggcagcatcc
    10501 ctactaaata tggcgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg
    10561 acattgcggt gctgttggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag
    10621 ctattcgcgt gcagcgtgta aatgctgatg accgtacgca aattgacgaa gaaatcattg
    10681 agtgtgaagg ctggacgcgt tacaccttcc ccgcgcgtgc cgggcaatac tcgaagttta
    10741 tctgggattt caaatgtttt agcggtatcg accatatcga aaaccctgat gaagatggca
    10801 tatttaaaat tgttaacgac tacaccggcg aaggctggaa cgatcaggtt gatgatgaat
    10861 taggtaattt cgattatctg atgggcgaga atatcgattt tcgcaatcat gccgtgacgg
    10921 aagagattaa atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc
    10981 ttgatgcggt caaacatatt ccggcgtggt tttataaaga gtggatcgaa cacgtacagg
    11041 aagttgcgcc aaagccgctg tttattgtgg cggagtactg gtcgcatgaa gtcgataaac
    11101 tgcaaacgta tattgatcag gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga
    11161 tgaaattcca tgaagcatcg cgcatggggc gtgactacga catgacgcag attttcacgg
    11221 gtacattagt ggaagccgat cctttccacg ccgtgacgct cgttgccaat cacgacaccc
    11281 aaccgttgca agccctcgaa gcgccggtcg aaccgtggtt taaaccgctg gcatatgcct
    11341 taattttgtt gcgggaaaat ggcgttcctt cggtattcta tccggacctc tacggtgcgc
    11401 attacgaaga tgtcggcggt gacgggcaaa cctatccgat agatatgcca ataatcgaac
    11461 agcttgatga gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt
    11521 tcgaccatcc gaactgcatt gcctttagcc gcagtggcac tgacgaatat cccggctgcg
    11581 tggtggtcat gtcgaacggg gatgatggcg aaaaaaccat taatctggga gagaattacg
    11641 gcaataaaac ctggcgtgat tttctgggga accggcaaga gagcgtagta accgacgaaa
    11701 acggtgaagc gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg
    11761 tgatttaaat tcatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt
    11821 aacggcgctt tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc
    11881 gtcataccat cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt
    11941 acggtaacgt tagtgagatc atcagacatc aatgtcaact tgcccggcac ggcaatcacc
    12001 cgctgccact gacggcaatc cagggtatcg ccctctttag tgacaatcag gctgcctatc
    12061 gcttccggac tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag
    12121 tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc
    12181 aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag
    12241 aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga
    12301 tctaactatt atttttgtga attattaccc cgaaaataaa tttgcatgat gtatgattcg
    12361 cgattcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca
    12421 aacacgcctg gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc
    12481 tctccactta ctattttggc attactggca ccttttgggc tgtcacgggt gaatttaccc
    12541 gttggggcgg tcagctcctg cagctgtttg gcgtccatgc tgaagagtgg ggttatttta
    12601 aaattatcca tctggaagga tcgccattaa cccgcatcga cgggatgatg atcctcggca
    12661 tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa tgtcaaactg cgcatgccac
    12721 gcagccgtat ccgcattatg caggccatta ttggcggtat tatcgctggt tttggcgcgc
    12781 gtctggcaat gggctgtaac ctggcggcgt tctttaccgg tattcctcag ttctcgctgc
    12841 atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgca cgctttaccc
    12901 tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga
    12961 cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggta
    13021 tgctgggctg ggcgctgctc acggcgatga accaaccaaa actggggctg gcaatgctgt
    13081 ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc
    13141 gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatc ggtatggcgg
    13201 tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacccaaa atcatgtggg
    13261 cgggaccaaa cgcggtaatt ggtggtttac tgtttggttt tggcatcgtg ctggcaggcg
    13321 gctgcgaaac cggttggatg taccgcgcgg tagaaggtca ggtgcactac tggtgggtcg
    13381 gtctgggcaa tgtgatcggc tcaaccattc tggcgtatta ctgggatgat ttcgctccgg
    13441 cgctggccac cgactgggac aaaatcaacc tgctgaaaac ctttggcccg atgggtggcc
    13501 tgctggtgac atatttgctg ttgtttactg cgctgatgtt gattatcggc tgggaaaaac
    13561 gcttcttccg ccgtgcggca ccgcagactg ctaaggagat cgcatgaaaa atatcattcc
    13621 tgattaccgt cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg caacccttga
    13681 ggcgatgccg cagttaaaaa aaggtgagat cctggaagtg gtgagcgact gtccgcagtc
    13741 gatcaataat attccactgg atgctcgtaa tcacggctat acggtgctgg atattcagca
    13801 agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg ctgtggccaa
    13861 cagcgggaat atcgttgcct ctgtaccaca tagattttac ccgccccctc tcctattctt
    13921 ttcagtgtgc tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga
    13981 agattacctt gcgcttctcg cggaagatat tgaacgcgat attccctatg atcccgaacc
    14041 cattggcaga tacaacgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga
    14101 acaccttcat ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc
    14161 gctgattaac gcccgcgtag aaactgcggc caccagtcgt atgtttaaac cgctctggca
    14221 acatggtcgg gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa
    14281 aaaacagcct tattttatct atcgtgctga cggacaacct gtttttatgg ccgcgatagg
    14341 tagcacaccg tttgagcgcg gtgacgaagc cgaaggattt ttaattgtca ctgcagcagc
    14401 agaccagggt ctggtagata ttcatgaccg ccgcccgctg gtactgtcgc cagaagctgc
    14461 gcgggaatgg atgcggcaag agattggcgg taaagaagca tcagaaattg ctaccaacgg
    14521 ctgtgtgcca gcaaaccagt tcacctggca cccggtatcg cgcgcggtag gtaatgtcaa
    14581 aaaccagggc gcggagttaa ttcaaccggt ttgataagcc aggagactat tgatcaataa
    14641 tactgcgtca taagaaatct ctattagaca aagtagacgc tccttccaca tgcgccacaa
    14701 gaaacctcta ttccagtgac acaattacgc ctgattattt ccctagcccg gaatatgaat
    14761 aaaaacatca acaaagatta aattattcaa acgttactgt tgcgtctgcg gtcgtaacaa
    14821 catccgacgt ggtgatgtgt atcaaatgag gcctctgatg atcagagggt ttattgcgta
    14881 tacggatcgt ggatggcaga aaactttcag gatcaggtat ccattttaaa ccagaaatag
    14941 ggtgagtttg ccccatccat gccccacgcg ggcagatggg atgtaattaa caacaggtta
    15001 caaaacctac acttgcatgg acatcacttc ctgatacgcc gccaccagct tattacgcac
    15061 ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca catcgtttaa
    15121 cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcag ctgtttgtgt
    15181 atcgctaatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg
    15241 cagtgaatcc tgcgcgcgcg cactcatcgc cgtagcctgt aactggctga taaccccttc
    15301 aatcccctgt atcgctgaca ttttcatctc ctggataatt tctggtagca aagcctacca
    15361 gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa acgggtttat tggcggatag
    15421 aaaaaaacga aagcacaaat aatgggagcg tcaatttttc gagtttgctg acccgggagt
    15481 gagtcttgtt ccactttgcc aataacgccg tccataatca gccacgaggt gcgcgatgaa
    15541 tgcgactgca gcccagacaa aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa
    15601 aattccattg attgttgccg gttccgcggc agtggcggtc atggtcgcac tgatcctgtg
    15661 ggcgaaagcc cccgactacc gcacattatt cagcaatctt tccgatcagg atggtggcgc
    15721 aattgtcagc caactgacgc aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc
    15781 tattgaagtt ccggcagata aagttcacga actgcgtctg cgcctggcac aacaaggttt
    15841 gcccaaaggc ggcgcggtcg gtttcgaact actcgatcag gaaaagtttg gtatcagcca
    15901 gttcagcgaa caggtgaatt atcagcgggc gctggaaggc gagctttctc gtaccatcgc
    15961 aactatcggc ccggtaaaag gggcgcgcgt acatctggca atgccgaaac cgtctttatt
    16021 cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta aatctgttac ccggccgcgc
    16081 actcgatgaa gggcaaatta gcgccattgt gcatctggtt tccagcgccg ttgctggtct
    16141 gccgccggga aacgtcacgc tggtggatca gggcggacat ctgttaaccc agtccaatac
    16201 cagcgggcgc gatcttaatg atgctcagtt gaaatatgcc agcgatgtcg aaggccgtat
    16261 tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt aacggtaata ttcacgccca
    16321 ggtcacggcg cagctggact tcgccagtaa agaacaaacg gaagaacagt atcgccctaa
    16381 cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg agcaaagcgg
    16441 ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggcac ctgcgaataa
    16501 cgcgccaatc agcacgcctc cagcaaatca aaataaccgc cagcagcagg cgagcaccac
    16561 cagcaatagc gggccgcgta gcacacagcg gaatgaaacc agtaactacg aagtcgatcg
    16621 caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt
    16681 ggtgaattac aaaaccttgc cagacggtaa accattgcct ctcagcaacg aacagatgat
    16741 gcaaattgaa gatctgaccc gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct
    16801 caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc ggcggagaac tgccgttctg
    16861 gcaacagcaa gcgtttatcg atcagttgct ggctgccggt cgctggttgc tggtactgct
    16921 ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag ttaacacgtc gcgccgaggc
    16981 gatgaaagct gtacagcaac aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt
    17041 ccgcctgagc aaagacgaac aactccaaca acgacgcgct aaccaacgtc tgggggcaga
    17101 agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt
    17161 cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca
    17221 tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc
    17281 gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc
    17341 taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgccgca ctgaatatca
    17401 acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaggaa cgtgccgcca
    17461 gcctgctgga agatattctc gaaactcgcg ataccgccag cggtattgaa acgctcaact
    17521 ttatggagcc gcagagtgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca
    17581 ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac
    17641 gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc
    17701 tggcggaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca
    17761 aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaact cagcaggaag
    17821 aagccgttat taccgccgtg cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg
    17881 agatgttcct gttcgaaaat ctggtggatg tcgacgatcg cagcattcag cgtctgttgc
    17941 aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg
    18001 agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca
    18061 accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg
    18121 tgcgccgcct tgccgaaact ggcgagatgg taattggcag cggcgaggat acctatgtct
    18181 gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccaca ggcagagttt
    18241 gtgcccatgg tcgagccgga agaaaccatc attgaagagg ccgaacccag ccttgagcag
    18301 caactggcgc aactgcaaat gcaggcccat gagcaaggtt atcaggcggg gattgccgaa
    18361 ggtcgccagc aaggtcatga gcagggctat caggaaggac tggcccgggg gctggagcaa
    18421 ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg cccggatgca gcaactggtc
    18481 agcgaatttc aaactaccct tgatgcgctt gatagtgtga ttgcttcgcg cctgatgcag
    18541 atggcgctgg aggcggcacg tcaggtcatc ggtcagacgc caacagtgga taactcggca
    18601 ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg taaaccacag
    18661 ctgcgcgtgc acccggatga tctgcaacgt gtggatgata tgctcggcgc aaccttaagt
    18721 ttgcatggct ggcgcttgcg gggcgatccc acactccatc ctggcggctg taaagtctcc
    18781 gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg
    18841 gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata
    18901 actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctatgggcga ttaacccgcg
    18961 ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca
    19021 ttgaacgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg
    19081 gtcaacggct gtttttaatg ccgctggagg aagtcgaagg tgtcctgccc ggcgcgcggg
    19141 tttatgccaa aaacatttcg gctgaaggac tgcaaagcgg caagcagttg ccgctcggtc
    19201 cggcgttatt aggtcgcgtt ctggacggca gcggtaagcc gctcgatggc ctgccctccc
    19261 ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac
    19321 cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgttgggc
    19381 gtgggcagcg tatgggactg tttgccgggt ccggcgttgg taaaagtgtg ctgctgggga
    19441 tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgcggac
    19501 gcgaagtaaa agattttatt gagaatatcc tcggtgccga agggcgtgca cgctccgtgg
    19561 tgattgccgc tccggcggat gtttctcctc tcctgcgaat gcagggtgcc gcctatgcca
    19621 cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc
    19681 tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacccgcca
    19741 ctaaaggtta tcccccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggaa
    19801 atggcattag cggcggcgga tcgattactg cattttatac cgtgctcact gaaggcgatg
    19861 accagcagga ccccattgcc gactctgcgc gggcaatcct cgacggtcac attgtgctgt
    19921 ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagcc
    19981 gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc
    20041 tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca
    20101 gcgatccgat gctcgacaaa gccatcgccc tgtggccgca gctggagggc tatttgcaac
    20161 aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc
    20221 cgacagtgtc ataacccagg agataacggc agatggcaga acatggcgcg ctggcgaccc
    20281 tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg cctgctgggt gaaatgcgtc
    20341 gcgggtgtca gcaggcggaa gaacagctca aaatgctgat cgattatcag aatgaatatc
    20401 gcaataacct caacagcgat atgagtgccg ggatgaccag caaccgctgg atcaactatc
    20461 agcagtttat ccagacgctg gaaaaagcca ttactcagca tcgccagcaa cttaatcagt
    20521 ggacgcagaa agttgacatt gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg
    20581 cctggcagac actgcaggaa cggcaatcca cggcggcact gcttgcagaa aaccgcctcg
    20641 atcagaaaaa gatggatgag ttcgcccagc gcgccgccat gaggaaacct gaatgattcg
    20701 cttagcgccc ttaattaccg ccgacgttga caccaccaca ttgcctggcg gcaaagccag
    20761 cgatgctgca caagattttc tcgcgttgtt gagcgaagca ttagcaggcg agacaactac
    20821 cgacaaagcg gccccccagt tgctggtggc aacagataag cccacgacaa aaggcgagct
    20881 gctgatcagc gatattgttt ccgacgcgca acaagcggat ttactgatcc ctgtggatga
    20941 aacactgcct gtcatcaacg tcgaacaatc cacatcaaca ccgttaacca ccgctcagac
    21001 gatgacgttg gctgcggtgg ctgacaaaaa tacgacaaaa gacgaaaaag cggatgatct
    21061 gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg atgttgccgg gttttgacaa
    21121 tacgcccaaa gtgactgatg tgccgtcaac cgtgttaccg gcagagaaac caacgctctt
    21181 cacaaaactg acttctgcgc aactcacaac agcacagcct gatgatgccc ccggcacgcc
    21241 agctcagcca ttaacaccgc tggtagcaga agcccagagt aaagcggaag tcatcagcac
    21301 accgtcaccg gtgaccgctg ccgccagccc gctaattact ccacaccaga cacagccact
    21361 gcccaccgtc gccgcgcctg ttttgagtgc accgctgggt tctcacgaat ggcaacaatc
    21421 attaagccag catatttcgc tattcacccg ccaggggcaa caaagtgcag agttgcgtct
    21481 gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa gtggatgata accaggcaca
    21541 aatccagatg gtttcaccgc atcagcatgt acgcgccgcg ctggaagcag cgcttccggt
    21601 actgcgtacg cagctggccg aaagtggcat tcagttaggg caaagcaaca tcagtggcga
    21661 aagctttagt ggtcagcagc aggccgcttc ccaacaacag caaagccaac gcacagtaaa
    21721 ccatgaacct ctggcggggg aagacgacga tacgcttccg gttcccgtct ctatacaagg
    21781 gcgcgtaaca ggcaacagcg gcgttgatat tttcgcctaa cgtcagaggt agcaccgtaa
    21841 tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg ataattagcc gataagcagt
    21901 agcgacacag gaagaccgca acacatgact gattacgcga taagcaagaa aagcaagcga
    21961 tcgctttgga tcccgattct ggtattcatt accctcgcgg cctgtgccag cgcaggttac
    22021 agctactggc attcgcatca ggttgccgct gacgacaaag cgcagcaacg cgtcgtaccc
    22081 tccccggtct tctacgcgct ggataccttc acggtcaatc tgggcgatgc ggatcgcgtg
    22141 ctttatatcg gcgtaaccct gcgcctgaaa gatgaagcta cccgctcgcg gctgagtgag
    22201 tatttgccgg aagtccgtag ccgtttgctg ttactgtttt cgcgtcagga tgctgccgta
    22261 ctggcgacag aagaaggcaa gaaaaacctg attgccgaga ttaaaaccac gctttccacc
    22321 ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg tgctgtatac cgcttttatt
    22381 ctgcgataac gacatgggcg atagtattct ttctcaagct gaaattgatg cgctgttgaa
    22441 tggtgacagc gaagtcaaag acgaaccgac agccagtgtt agcggcgaaa gtgacattcg
    22501 tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat
    22561 cattaatgag cgctttgccc gccattttcg tatggggctg ttcaacctgc tgcgtcgtag
    22621 cccggatata accgtcgggg ccatccgcat tcagccgtac catgaatttg cccgcaacct
    22681 gccggtgccg accaacctga accttatcca tctgaaaccg ctgcgcggca ctgggctggt
    22741 ggtgttctca ccgagtctgg tgtttatcgc cgtggataac ctgtttggcg gcgatggacg
    22801 cttcccgacc aaagtggaag gtcgcgagtt tacccatacc gaacagcgcg tcatcaaccg
    22861 aatgttgaaa ctggcgcttg aaggctatag cgacgcatgg aaggcgatta atccgctgga
    22921 agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc aatatcacca cctcgccgaa
    22981 cgacattgtg gttaacacgc cgttccatgt ggagattggc aacctgaccg gcgaatttaa
    23041 tatctgcctg ccattcagca tgatcgagcc gctacgggaa ttgttggtta acccgccgct
    23101 ggaaaactcg cgtaatgaag atcagaactg gcgcgataac ctggtgcgcc aggtgcagca
    23161 ttcacagctg gagctggtcg ccaactttgc cgatatctcg ctacgcctgt cgcagatttt
    23221 aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt
    23281 tgacggcgtc ccggtgctga ccagtcagta cggcactctc aacggtcaat atgcgttacg
    23341 gatagaacat ttgattaacc cgattttaaa ttctctgaac gaggaacagc ccaaatgagt
    23401 gacatgaata atccggccga tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg
    23461 ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg acgcggtgtt ccagcaattt
    23521 ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc tgataatgga tattccggtc
    23581 aagctgaccg tcgagctggg ccgcacacga atgaccatca aagagctgtt gcgtctgacg
    23641 caagggtccg tcgtggcgct ggacggtctg gcgggcgaac cactggatat tctgatcaac
    23701 ggttatttaa ttgcccaggg cgaagtggtg gtcgttgccg ataaatatgg cgtgcggatc
    23761 accgatatca ttactccgtc tgagcgaatg cgccgcctga gccgttagtg atgaataacc
    23821 acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc actgctgcag gtgagcggcg
    23881 cactcatcgc cattattgcc ctgatcctcg ctgctgcctg gctggtaaaa cggttgggat
    23941 ttgtccctaa acgcactggc gttaacggtc tgaaaattag cgccagtgct tcgctgggcg
    24001 cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg
    24061 cagggcaaat caatctgctg cataaacttc ccccttcagc accaacggaa gagataccgc
    24121 agcccgattt tcagtcggtc atgaaaaact tgcttaagcg tagcgggaga tcctgatgcg
    24181 tcgtttgttg tctgtcgccc ctgtccttct ctggctgatt acgcccctcg ccttcgcgca
    24241 actgccgggt atcaccagcc agccgctgcc tggcggtgga caaagctggt cgctcccggt
    24301 gcagacgctg gtgttcatca cctcgttgac gtttattccg gcaattttac tgatgatgac
    24361 cagtttcacc cgcatcatta ttgtttttgg tttattgcgt aacgcgctgg gaacaccctc
    24421 cgcgccacct aaccaggtat tgctggggtt ggcgctgttt ttgacctttt ttattatgtc
    24481 gccggtgatc gacaaaattt atgtcgatgc gtaccagcca ttcagcgaag agaaaatatc
    24541 aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt gagtttatgc tgcgtcagac
    24601 ccgtgaggcg gatttagggt tgtttgccag actggcgaat actggcccgt tgcagggacc
    24661 tgaagccgtg ccgatgcgca ttttgctccc ggcctacgtg accagcgagt tgaaaaccgc
    24721 atttcagata ggcttcacga ttttcatccc ttttttgatt atcgacctgg tgattgccag
    24781 cgtgttgatg gcattgggga tgatgatggt cccccccgcc accattgctc tgcccttgaa
    24841 actgatgctg tttgtactgg tggatggctg gcaattgctg gtcggttcgc tggcgcagag
    24901 cttttacagc tagagaggca aaatgacacc tgaatcggtc atgatgatgg ggactgaagc
    24961 gatgaaagtc gcgctggcac tggctgcccc gctgttgctg gtggcgctgg tcacgggcct
    25021 tatcatcagt attttgcagg ccgccacgca gatcaacgaa atgacgctgt cgtttattcc
    25081 aaaaatcatc gccgtattta ttgccattat tattgccgga ccgtggatgc tcaatctgtt
    25141 gctggattac gtccgcacct tgttcactaa cctgccgtat atcatcgggt agcccgtaca
    25201 atgatgcagg tgacaagcga tcaatggctt tcctggctaa gtctgtactt ctggccgtta
    25261 ctgcgcgtgc tggcgctcat ttctaccgcg ccaattctga gcgaacgcag cgtgccgaaa
    25321 cgggtaaaac tgggtctggc aatgatgatc acgttcgcta ttgccccatc attacctgcc
    25381 aacgatgttc ctgttttttc gttctttgct ctgtggctgg ccgtgcagca gatcctgatc
    25441 ggcattgcgc ttggtttcac catgcaattt gcctttgccg ctgtgcgaac cgctggcgaa
    25501 attatcggtc tgcaaatggg gctgtcattt gcgacgtttg tcgatccggc cagccatctc
    25561 aacatgcccg ttctagcgcg tatcatggat atgctggcgt tactgctgtt cctgacattt
    25621 aacggtcatt tatggttgat ttcactgctg gtcgatacct ttcacaccct gccgattggt
    25681 ggcgaaccgt tgaacagcaa tgcgtttctg gcactcacca aagcagggag tttgattttc
    25741 cttaacgggc tgatgctggc gttaccgctc attactctgc tgctgaccct gaatctggca
    25801 ttaggtttac tgaatcgtat ggccccgcaa ttatccattt ttgttattgg atttccatta
    25861 actctgactg tcggtatctc tttaatggcg gcattaatgc cgttaattgc gcctttttgc
    25921 gaacatttat tcagtgagat gtttaatttg ctggctgata ttattagtga attgccatta
    25981 atataattcc gtaacgttta tcatgttatc ctaaggatta tccgaaaaat aatacctacg
    26041 aacatcttcc aggatactcc tgcggcgaaa tatttgtttt aagctcactc acatatcgca
    26101 acatttactt tactttaaga caattccagg caaattatac aacactttac gggatagtaa
    26161 gtccgcctga aaaatcgcga gagtggcgca ttaggtgacc catgttgttc cgtttagtca
    26221 tgatgaaata ttcaggtaag gggaattatc gttacgcatt gagtgagggt atgccatgtc
    26281 aacgattatt atggatttat gtagttacac ccgactaggt ttaaccggat atctgttgag
    26341 tagaggggtt aaaaaaagag aaatcaacga cattgaaacc gttgatgacc ttgccatagc
    26401 ttgtgattca cagcgccctt cagtggtgtt tattaatgag gactgtttca tccatgatgc
    26461 ttctaacagt cagcacatca agcacatcat taatcaacat cccaatacgt tatttatcgt
    26521 ttttatggca attgccaatg ttcattttga tgaatatcta ttggtcagaa aaaatttatt
    26581 gataagttct aaatcgatta aaccggaatc tctcgacgat atccttggcg atattctgaa
    26641 aaaagagaca acgataacct cttttttaaa tatgccgacg ttatcattga gccgaaccga
    26701 atcgagtatg ttgcgaatgt ggatggcagg tcagggaacg attcaaatct ctgaccaaat
    26761 gaatatcaaa gccaagaccg tttcatcgca taaaggtaat attaaacgta agatcaaaac
    26821 gcataataaa caggttatct accatgtcgt ccgactgacg gataatgtga ctaatggtat
    26881 ttttgtcaac atgcgctaac acattctgac tggtggtttc ccaccagtca ggctgaataa
    26941 gattactctg ctttctccac aaagataccg tcctgatgcc ctgcttcatt aaagaaccag
    27001 atgccgagcg ggtagtcttc cagcgaaacc aggtacattg tgccttcact aaactcctca
    27061 actgccagta ccacgccagg acgacgcgga ccgccatccg ttttgactgt tacccgatca
    27121 ttcaccttca tcgttttcct cctgtggctt tgtgccagtg tagaacaatt tcgtttttct
    27181 ggcagcgcca ggcgcgcgcg agtgctgatt ttctcgacgg tctatactta agagatgcca
    27241 ggcggactta acgactggcg gcaacaacag agtaacggtt gcgaggaaag atgatgaaaa
    27301 ccgcaaaaga gtacagcgat accgcaaaac gtgaggtcag cgtcgatgtc gatgccctgc
    27361 tggcggcgat caatgaaatt agcgaaagcg aagttcatcg cagccagaac gattctgaac
    27421 acgttagcgt cgatggacgt gaatatcata catggcgtga attggcggat gccttcgaac
    27481 tggatattca tgacttcagc gtctctgaag tgaatcgttg aatgcacaat aaaaaaatcc
    27541 cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga agcacttaaa aaattcgtta
    27601 caccaggaaa tctgatgtgt tcatcacctt atccgcaatt tttttcgctg acaagaaaat
    27661 attcgcgata tgaatgatta acgctactta tgaatagaaa tgtgacgtgc gtcacccctt
    27721 ggtggctctg gcgtcaacgg gatcgctccc gaaaaaatca tctttgatta tttgtgctgt
    27781 tttgagaatc atcctggtaa ggggagaact gcgcgaccat gttttcaatt caacaaccac
    27841 tactggtttt tagcgatctt gatggcaccc tgctggacag tcatagttat gactggcaac
    27901 cagctgcccc ctggctcagc cgtttacgcg aagcgaatgt tcccgtcatt ctctgtagca
    27961 gtaagacatc agcggaaatg ttgtacttgc aaaaaacgtt ggggctacaa ggtttaccgc
    28021 tgattgccga aaatggcgca gtgatccagc ttgctgagca atggcaggat atagacggtt
    28081 ttccacgcat catctcaggt attagccatg gcgaaatcag ccaggtatta aatacgctac
    28141 gcgagaagga acattttaaa ttcacgactt ttgatgatgt cgacgatgca accatcgccg
    28201 aatggacggg attaagccgt agccaggcgg cgctgacgca gctgcatgag gcgtcggtaa
    28261 cgctaatctg gcgcgacagt gacgagcgta tggcacaatt taccgctcgt ctgaacgaac
    28321 tgggcttaca gtttatgcag ggtgcgcgct tctggcacgt cctggatgcc tctgccggaa
    28381 aagatcaggc cgccaactgg attatcgcga cctatcaaca attatcaggc aaacgcccaa
    28441 ccacacttgg cctgggcgat gggccaaacg atgcgccctt actggaggta atggattacg
    28501 cggtgattgt gaaagggcta aaccgtgaag gggtgcatct gcatgatgag gatccgaccc
    28561 gcgtctggcg aacgcagcgt gaaggaccgg aaggctggcg tgaagggctg gaccattttt
    28621 tctccgctcg ttaagcgtta tcgctcgcgc atacccgatt acgcccggcc tgtttagcga
    28681 gataaagccg acggtcagcc agtgactgga gttgttcaaa atcataatca ccggtttcct
    28741 cgctgctact aacccccaac gaggcactga tgcgtatcgt cgtactctta gcaatcaaca
    28801 tctctttttc atttaacttc aggcgaatac gttctgcgac ttccgcagcc tccgtcagac
    28861 tcgcgcctgg cagaatcaca caaaactcct caccaccgac ccgcccggca acgtcctgcg
    28921 cacgcaagga actgctaatt aatccggcag catgagaaag aacacggtcg cccgcctgat
    28981 gaccaaagcg gtcattaatc gctttaaaat ggtcaagatc gacctgaatg acagaaaaag
    29041 gatgttggtg cgtctgacac aatttagcga gcggacgggc tttttcgaac agtgcgccac
    29101 gattatataa acgggttaag gtgtcgtgcc acgcctgcca ctgcaacgag ctttgcagaa
    29161 catacatgtt gctgaccatc cggcgaatca cataccagga gatgagtaac atggtggtaa
    29221 agagcgccca cagcagggtt aatgcaatgc tgatactacc gaaatcgccg cgcacgcctt
    29281 cgcttagcgt atggacgcgc accagcacac cgtcgaaatg gtccagacgt tcccagctaa
    29341 catagcgact gtccatacga atgccgcccc gcgtgtcatg ttccatcgcc tgcgccagca
    29401 aggccagttc acgaggatca aaaatattcc ctgttggatg gtcaggattg gaagaggtca
    29461 aaaatctcag cttactgtca tagagctgat actcaccatc gaggttttta tcgatggcgt
    29521 ttcttaaaaa ttgctgcata gtacgcacag gaatactcat ccccagcacg ccataccagt
    29581 agttattact gtctaccgga acactgacgg taacctgcgg ttcagtattg ctggcgtgtt
    29641 ccggttgcga agtaaaccag cgtaccgcgc ggtgacgatt ttctcgttgc gaatggccga
    29701 taaaccaggg ttgggtgaca tagccgtaat aacgcgttgg aacattgcgc gtaaacaagg
    29761 tcggctgcgt cgaaacgtaa aatccggcac gcgagacata catcgcctgt tcaaccatcg
    29821 acgaggagtt gtgcgccagt cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat
    29881 tgtcgaggct ttcattttcg cgagacagga gattcccctc gctgactaat gcatccgaca
    29941 caccattaaa tggcagagtg cgccgtcggt tgagttcaat ttgccaggcg tgctcgtcgc
    30001 gatgctgttc gaactcggtt acagcattac gcagagaggt gaaatccagt ggcgctacga
    30061 gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct
    30121 tatcgagcgc gttggctacg ttttccagat gattacgctg gctggagata taggcatctt
    30181 ccagcaccac tacttcacgc caggtgagca aggtggaaaa aagcaatacc acgataaagc
    30241 agagattaac gacatgacca ggccccaggc ggcgtgcgag ttttttcaac cagctctggt
    30301 tttccatttt tgtctcgtgc tgcaccctga cgactcccaa cctgcttttt tatgattctg
    30361 gcataacctg gcgatagcgt accgcaaaac agcgataatt ttataatggt ggcatgataa
    30421 gacgcgacta tcattcattg caggcattgg cacaccaggc cggatgtgat ccggcctggt
    30481 gttaatgata ccctttcaaa cttcttccgc agaacgactc ccctcccccg gaacaagctc
    30541 ggtttcatga aaagcttccc gcttgacccc gtaaccgtca taccaacgac actcaaccat
    30601 accgctggag tatccggtga caatcatccg cgggccaccc tctttaaccg taacttcctc
    30661 actaaccata aagctcatac tcgcctcctt ttttcgagtg aaacttgttc accttagttg
    30721 aagatggcga attttgcata atagcctgtg cgagtattaa tgtgcctgac cgcgcatttt
    30781 cgctacggct ttcactccca gcaccacgat gccgccgatg ataaatccaa gaatcagatt
    30841 taaaacagtc ggtaatatca tcgccaccac tgcactttgc tgcccggcga aatgttcaat
    30901 ggcgtgatgc agcggcgcaa taccatgtac cacaatcccg ccgccgacga ggaacatcgc
    30961 cagcgtgccg acaatcgata acgccttcat caaccagggc gcaatgatca ataacccttt
    31021 acctaatgcc tgcatcagcg cgctggattt ttccgccagc caatacccca ggtcatcaat
    31081 cttaacgata acccctacca gaccgtagac gcccacggtc actaccagcg cgatgcctga
    31141 aagcaccagc acctgattaa gcaacggcgc ttcggccaca atccccagcg tgatggcgac
    31201 gatttcggca gacaatataa aatcggtacg aatcgcccct tttattttgt ccttttcaaa
    31261 cttcagcgga tcctgcgccg ccagcttctc cagacgctgc tggctctgcg ccggatcttc
    31321 tttatgttta cgcgcctcca gcatatgcag cactttctct actccttcaa agcagagaaa
    31381 cgcgccacca atcatcaaca gaggcgtaat cgcccacggg ataaacgcac tgatgatcag
    31441 cgccagcggc accagaatca ctttattaat cagcgatcct ttcgccacgc cccagaccac
    31501 gggaagttcc cggttggccc gcacgcctga aacttgttgc gcattgagcg ataagtcatc
    31561 ccctaataca ccggcggttt tcttcgccgc cagtttgccc atcacggaga tatcgtccag
    31621 tagtgtggcg atatcatcga gcaacgtcag taaactactc ccggccaaaa gcattccctc
    31681 tcatttttat tgttgaaggg tgtaagtatg aagcaaaaac ggcagctctg atacttgctt
    31741 cacaggtcaa cattttttta acaattaagt gcaattatat ctctcgccag cagaatgatt
    31801 ttcacgttta ctatgaacca cctttttatt tccaccgtga gggattatgc gtttccgcca
    31861 gttgttaccg ctttttggcg cgctgtttgc gttgtatatc atttggggct caacctattt
    31921 tgtcattcgg attggcgtgg aaagctggcc tccgttaatg atggcaggcg ttcgattcct
    31981 ggcagccggt attttattga tggcattttt gctactgcgc ggacacaagc tccccccgct
    32041 acgtccgctg ctcaatgccg cgctgattgg cctgttattg ctggctgtcg gtaatggcat
    32101 ggtgacggtt gccgagcatc aaaatgttcc ttccggcatc gccgccgttg tggttgcaac
    32161 cgtgcccctc tttaccctgt gcttcagccg cctgtttggc attaaaacgc gcaaactgga
    32221 atgggtgggt atcgccattg ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt
    32281 aagcggcaat ccgtggggcg cgattctgat tttaatcggc tcgattagct gggcgtttgg
    32341 ctcggtttat ggctcgcgca ttaccttacc tgtagggatg atggcgggtg ctattgagat
    32401 gctggcggca ggcgtggtgt taatgatcgc gtcgatgatt gcaggtgaaa aactgacggc
    32461 gctcccttcc ctttcaggct tccttgcggt cggctatctg gcgctgtttg gttcgattat
    32521 cgccatcaac gcttatatgt atttaatccg taatgtcagt ccggctctcg ccaccagcta
    32581 cgcctacgtt aacccggtgg tcgcggtctt gctgggtacg ggactgggcg gagagacgct
    32641 gtcgaagatt gaatggctgg cgctcggcgt aattgtcttc gcggtggtgc tggtcacgtt
    32701 gggaaaatat ctctttccgg caaaacccgt agttgcgcca gttattcagg acgcatcaag
    32761 cgagtaaatg aatcccctgc gtgtcgatct gcgcgctggc accttcgccg cagatccact
    32821 cttccagacg ctcgctaagc gcttcatccg tcagcttctc gcgcccacgt aacgcgcact
    32881 cccagacaat caatacgcgc cagccgagtt cctgcaagcg actgatatcg cggcgatcgc
    32941 gctcaacatt tttacctatc ttctccagcc agaactcggt tcgagtcgca ggcactttaa
    33001 acagatagca gtgatgatga tgccagaagc agccgtgggt aaatatcacg cagcgatatt
    33061 catcaacgac aaaatccggg cgtccgggca gactggcgtc ctgaacgcga aacgccaggc
    33121 cctgcccggt taacagactg gcgaggcgct tctctatcgc cgtatcacgc gtggcaatcg
    33181 cgcgcatatt tttgctgcga gtggccttat cgtgaacgtc ggccatgttg tgcctcttgc
    33241 tgacgcaacg ccaccgcctg tttgattttt ggctcaagca gttttgccac cgcggcaaag
    33301 accggcacga ccaccgagtt accgaactgg cgataggcct gagtgtccga aaccggaatg
    33361 cggaatttcg cttctcccgg cgcttcaaaa cccattaagc gcgcgcattc acgaggcgtt
    33421 aaccgacgtg gacgatgttg ctgattctgc ggatcgtcaa agtctttctc acctttggcc
    33481 atatcccagc cgcgatcgat taaaatttcc gcgccatctt tgtaataacg cgcagacagc
    33541 gtacgcgtga cgctttgcgg attgttcgga taaaccattc cataaccgaa gccgttaccg
    33601 cgcgcctgat gttttttcgc atatcgatag aggtacttcc acagcaccgg cgtcaggata
    33661 tatttcgcct cgaccatcgg gtccagcagc tgcgccagcg tcactcgctg cgcagggaaa
    33721 cattcgctga tatcacgcag ggtaaaatcg gctttcagat tcagatcgcg acgaaaaccc
    33781 accagcacga tgcgttcacg gtgctgtggc agaaaatgtt tgccgtcgat gattttcgga
    33841 tcgtctggcc cgttatcttc tgcatcagcc tcgtcatagc ccagttcgtc cagcgtctgc
    33901 atgatgatgc ggaacgtttt accctggtca tggcttttca ggtttttgac gttttcgagc
    33961 acaaacatcg ccggacgacg cgagtcgata atgcgcacca catcaaaaaa cagcgtgccc
    34021 tgggtatcgc aggcaaaacc gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc
    34081 agcgaaaatg gctgacaagg gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga
    34141 cgaatatgtt ccgccgccgc ctcatcactc acgccttctt tatggctgag ggtgatgtcg
    34201 cggatatctt cattaaaatg atgcgtcgcc gggtcgcaat aatgattggc tttataagtg
    34261 cgtaccgcat gtttgttcca ttcgctggta aacacgcact gtccgccaat cgattcaaaa
    34321 ccgcgacgga tgccgccaat tccggcaaat agatcgataa agcgaaacgc ataatgcgga
    34381 tgatgtgccg gtggtttggg taacaacgtt tgcagatggg cgaactcttt ctcacttaaa
    34441 cggtgccatg ccgagtcatt cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc
    34501 tcacccacac cattaagctg cgccaccagc gttttaacat cataaatttg cagcagtttc
    34561 tccagcattg cctgtgcggc attcccggtg ctgtatgaat cggttactga tatattttcc
    34621 tgcatagatt taaccggcca tctgagatgg ccgacaggtt aacataatta cagccattta
    34681 gggccacgcg cttatcccgc gggtgaaaac gcatctatca ccttgtgatc gacaccttca
    34741 ttctcccccg ccagttcggc actgagcttc gccataaact cgactaaaaa atgcgcgttg
    34801 tgctgcgcca gctgcttgcc tcttgcggtt tgcatggttt gcggtagctt cagcaactta
    34861 gtctggaaat gatccagcgc gtagcgttta tcatcaagcg ggcgatgctg tgcgaacgga
    34921 tcttcaccat caaacagcgc cacgcccaat gcccctgaaa cggcaaacac gcgcgccagg
    34981 ccaatcgccc ccagggcttc cagccgatcc gcatcctgga cgattttagc ttccgttgtt
    35041 aagggggcga tttgcgcgct gaaactgtga gcggcaatgg catgacaaac ggcctcgatt
    35101 ttctccgccg gaaattgctc gaactcttca cggagcaggc gacgcgtctc ttctgccgcc
    35161 aggattgaag aacgctgcct ttgcggatga tttttcgcca ggctcacaat atcgtgaaaa
    35221 taacaggcgg ttaaaatcac cagcatatcg acatcgtcat ctgccgccag cttctgtgcc
    35281 gtagcccaga cgcggcgaaa atgacacacg tcatgggccg cgccctggtg ctgatgatgg
    35341 tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg cgctccgttg
    35401 ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc gggacgaggt
    35461 cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa taaaaatccg
    35521 accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa tcccgacacc
    35581 ggggcatcgg ggatccggct catcacccag atgtaacgcg ccattgccca gacgaaatag
    35641 ccacaccatg tatagaaagc ccacaaagcc agtttgccac cagggctgcg agagagtctc
    35701 tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt cagacttagc
    35761 actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa gactcactca
    35821 ttttttgaac aaatagataa tttttgcgca acggcagtga atacaggaaa aatcgttttg
    35881 agcgatttta tttatctttg agatcggctt tattaattca tttaatcaat atattagcac
    35941 tgaatacaat tatacctttt tatttttctg tgtcatgatg cctccgttat tagcctttta
    36001 tcgtcttgtt tatatttttt gggccggcat gatgccggct tttttttatg ccttcattaa
    36061 tgtgcgcgtg atcacaccag ccgtttggcg caacaatcat tgataccccc tatgcttccg
    36121 ct
//
LOCUS       DAZGCS010000046        35905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGCS010000046 DAZGCS010000000
VERSION     DAZGCS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.046"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..750
                     /gene="araD"
                     /locus_tag="ACHK01_002333"
     CDS             55..750
                     /gene="araD"
                     /locus_tag="ACHK01_002333"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8097279.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            825..3176
                     /gene="polB"
                     /locus_tag="ACHK01_002334"
     CDS             825..3176
                     /gene="polB"
                     /locus_tag="ACHK01_002334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO8097280.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3341..6247
                     /gene="rapA"
                     /locus_tag="ACHK01_002335"
     CDS             3341..6247
                     /gene="rapA"
                     /locus_tag="ACHK01_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO8097281.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6259..6918
                     /gene="rluA"
                     /locus_tag="ACHK01_002336"
     CDS             6259..6918
                     /gene="rluA"
                     /locus_tag="ACHK01_002336"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO8097282.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(7035..7850)
                     /gene="djlA"
                     /locus_tag="ACHK01_002337"
     CDS             complement(7035..7850)
                     /gene="djlA"
                     /locus_tag="ACHK01_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO8097283.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8105..10459
                     /gene="lptD"
                     /locus_tag="ACHK01_002338"
     CDS             8105..10459
                     /gene="lptD"
                     /locus_tag="ACHK01_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO8097284.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10512..11798
                     /gene="surA"
                     /locus_tag="ACHK01_002339"
     CDS             10512..11798
                     /gene="surA"
                     /locus_tag="ACHK01_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO8097285.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11798..12787
                     /gene="pdxA"
                     /locus_tag="ACHK01_002340"
     CDS             11798..12787
                     /gene="pdxA"
                     /locus_tag="ACHK01_002340"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO8097286.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12784..13605
                     /gene="rsmA"
                     /locus_tag="ACHK01_002341"
     CDS             12784..13605
                     /gene="rsmA"
                     /locus_tag="ACHK01_002341"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO8097287.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13608..13985
                     /gene="apaG"
                     /locus_tag="ACHK01_002342"
     CDS             13608..13985
                     /gene="apaG"
                     /locus_tag="ACHK01_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO8097288.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13992..14834
                     /gene="apaH"
                     /locus_tag="ACHK01_002343"
     CDS             13992..14834
                     /gene="apaH"
                     /locus_tag="ACHK01_002343"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO8097289.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14912..15391)
                     /gene="folA"
                     /locus_tag="ACHK01_002344"
     CDS             complement(14912..15391)
                     /gene="folA"
                     /locus_tag="ACHK01_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO8097290.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15583..17445)
                     /gene="kefC"
                     /locus_tag="ACHK01_002345"
     CDS             complement(15583..17445)
                     /gene="kefC"
                     /locus_tag="ACHK01_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO8097291.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGFIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17438..17968)
                     /gene="kefF"
                     /locus_tag="ACHK01_002346"
     CDS             complement(17438..17968)
                     /gene="kefF"
                     /locus_tag="ACHK01_002346"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO8097292.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18076..19407)
                     /gene="yaaU"
                     /locus_tag="ACHK01_002347"
     CDS             complement(18076..19407)
                     /gene="yaaU"
                     /locus_tag="ACHK01_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097293.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(19466..19753)
                     /gene="fixX"
                     /locus_tag="ACHK01_002348"
     CDS             complement(19466..19753)
                     /gene="fixX"
                     /locus_tag="ACHK01_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO8097294.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19750..21036)
                     /gene="fixC"
                     /locus_tag="ACHK01_002349"
     CDS             complement(19750..21036)
                     /gene="fixC"
                     /locus_tag="ACHK01_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8097295.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21087..22028)
                     /gene="fixB"
                     /locus_tag="ACHK01_002350"
     CDS             complement(21087..22028)
                     /gene="fixB"
                     /locus_tag="ACHK01_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8097296.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22043..22813)
                     /gene="fixA"
                     /locus_tag="ACHK01_002351"
     CDS             complement(22043..22813)
                     /gene="fixA"
                     /locus_tag="ACHK01_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO8097297.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23287..24801
                     /gene="caiT"
                     /locus_tag="ACHK01_002352"
     CDS             23287..24801
                     /gene="caiT"
                     /locus_tag="ACHK01_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO8097298.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGCNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24832..25974
                     /gene="caiA"
                     /locus_tag="ACHK01_002353"
     CDS             24832..25974
                     /gene="caiA"
                     /locus_tag="ACHK01_002353"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO8097299.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26103..27320
                     /gene="caiB"
                     /locus_tag="ACHK01_002354"
     CDS             26103..27320
                     /gene="caiB"
                     /locus_tag="ACHK01_002354"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO8097300.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            27394..28947
                     /gene="caiC"
                     /locus_tag="ACHK01_002355"
     CDS             27394..28947
                     /gene="caiC"
                     /locus_tag="ACHK01_002355"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO8097301.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29056..29841
                     /gene="caiD"
                     /locus_tag="ACHK01_002356"
     CDS             29056..29841
                     /gene="caiD"
                     /locus_tag="ACHK01_002356"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO8097302.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            29847..30437
                     /gene="caiE"
                     /locus_tag="ACHK01_002357"
     CDS             29847..30437
                     /gene="caiE"
                     /locus_tag="ACHK01_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO8097303.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30523..30918)
                     /gene="caiF"
                     /locus_tag="ACHK01_002358"
     CDS             complement(30523..30918)
                     /gene="caiF"
                     /locus_tag="ACHK01_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO8097304.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(31179..34400)
                     /gene="carB"
                     /locus_tag="ACHK01_002359"
     CDS             complement(31179..34400)
                     /gene="carB"
                     /locus_tag="ACHK01_002359"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO8097305.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(34418..35566)
                     /gene="carA"
                     /locus_tag="ACHK01_002360"
     CDS             complement(34418..35566)
                     /gene="carA"
                     /locus_tag="ACHK01_002360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO8097306.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         8629 a         9680 c         9380 g         8216 t
ORIGIN      
        1 gctgcgattt tgtagaccgg ataagcaaag tgcatccggc acgaaggagt caacatgtta
       61 gaagatctca aacgcctggt attagaagcc aacctggcgc tgccaaaaca caacctggtc
      121 acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc gcggcgtctt tgtgatcaaa
      181 ccttccggcg tcgattacag cgtcatgacc gctgacgata tggtcgtggt tagcatcgca
      241 accggtgaag tggttgaagg tacgaaaaag ccctcctctg acacgccaac tcaccggctg
      301 ctctatcagg cattcccgtc cattggcggc attgtgcata cgcactcgcg ccacgccacc
      361 atctgggcgc aggcgggtca gtcgattcca gcaaccggca ccacccacgc cgactatttc
      421 tacggcacca ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg tgaatatgag
      481 tgggaaaccg gtaacgtcat cgtagaaacc ttcgaaaaac agggtatcga tgcagcgcaa
      541 atgcccggcg tcctggtcca ttctcacggc ccatttgcat ggggcaaaaa tgccgaagat
      601 gcggtgcata acgccatcgt gctggaagag gtcgcttata tggggatatt ctgccgtcag
      661 ttagcgccgc agttaccgga tatgcagcaa acgctgctgg ataaacacta tctgcgtaag
      721 catggcgcga aggcatatta cgggcagtaa tgactgtata aaaccacagc caatcaaacg
      781 aaaccaggct ataatcaagc ctggtttttt attggatttt caacgtggcg caggcaggtt
      841 ttatcttaac ccgacactgg cgggataccc cgcaagggac agaagtctcc ttctggctgg
      901 cgacggacaa cgggccgttg caggttacgc ttgcaccgca agagtccgtg gcgtttattc
      961 ctgccgatca ggttccccgc gctcagcata ttttgcaggg tgaacaaggc tttcgcctga
     1021 caccgctggc gttaaaggat tttcaccgcc agccggtgta tggcctttac tgtcgcgccc
     1081 atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg
     1141 ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca ccggtgtggg
     1201 tcgagggtga tatgcacaat ggcactatcg ttaatgcccg tctgaaaccg catcccgact
     1261 atcgtccgcc gctcaagtgg gtttctatag atattgaaac cacccgccac ggtgagctgt
     1321 actgcatcgg cctggaaggc tgcgggcagc gcatcgttta tatgctgggg ccggagaatg
     1381 gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc cagccgcccg ctgctgctgg
     1441 aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt gatcatcggc tggaacgtgg
     1501 tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtctt ccgctgcgtc
     1561 ttgggcgcga taatagcgag ctggagtggc gcgagcacgg ctttaaaaac ggcgtctttt
     1621 ttgcccaggc taaaggtcgg ctaattatcg acggtatcga ggcgctgaaa tccgcgttct
     1681 ggaatttctc ttcattctcg ctggaaactg tcgctcagga gctattaggc gaaggaaaat
     1741 ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataaac
     1801 ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa
     1861 ctgaaatcat gccattttta ctcgaacgag caacggtgaa cggcctgccg gtggatcggc
     1921 acggcggttc ggtggcagcg tttggtcatc tctattttcc gcgaatgcat cgcgctggtt
     1981 atgtcgcgcc taatctcggc gaagtgccac cgcacgccag ccctggcggc tacgtgatgg
     2041 attcacggcc agggctttat gattcagtgc tggtgctgga ctataaaagc ctgtacccgt
     2101 cgatcatccg cacctttctg attgatcccg tcgggctggt tgaaggcatg gcgcagcctg
     2161 atccagagca cagtaccgaa ggttttctcg atgcctggtt ctcacgggag aaacattgcc
     2221 tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata
     2281 aaccactttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg cttggcacca
     2341 ccgcctgccg ctttttcgat ccgcgcctgg catcgtcgat caccatgcgt ggtcatcaga
     2401 tcatgcggca aaccaaagca ttgattgaag cgcagggcta cgacgttatc tacggagata
     2461 ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga agaagaggcg gcgaaaatcg
     2521 gtcgtgtact ggtgcagcac gttaacgcct ggtgggcgga aacgctgcaa aaacaacggc
     2581 tgaccagcgc attagaactg gagtatgaaa ctcatttctg ccgttttctg atgccaacca
     2641 ttcgcggagc cgataccggc agtaaaaagc gctacgccgg gctgattcag gagggcgata
     2701 aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc
     2761 agcagtttca gcaggagcta tacctgcgca tcttccgcaa cgagccatat caggaatatg
     2821 tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga tgcgcgactg gtttaccgta
     2881 aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt gccgcctcat gtacgcgccg
     2941 ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat cagaatcgcg
     3001 gcaccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac
     3061 cactggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc
     3121 cttttattga ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa
     3181 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
     3241 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
     3301 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
     3361 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
     3421 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
     3481 ttcccccgtg acccgcgtga tgttcaaccc tggtgatact attaccagcc atgacggctg
     3541 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
     3601 cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt
     3661 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
     3721 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
     3781 gcgcggtcag cgtaccagcc tgattccgca tcagctcaac atcgctcatg atgttggtcg
     3841 ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
     3901 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
     3961 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
     4021 tgcactgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgtttga
     4081 caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac agcgcctgga
     4141 acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc acctggtgtg
     4201 gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag agcacgtgcc
     4261 gggcgttctg ctgctgaccg cgaccccgga acagctgggg atggaaagcc acttcgcccg
     4321 tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg aagagcagaa
     4381 aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa
     4441 tgacgaactg aacatgctcg gcgagatgat cggcgagcag gatatcgagc cgctgttgca
     4501 ggcagcaaac agcgacagcg aagatgccca gagcgcccgt caggagctgg tttcgatgct
     4561 gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg
     4621 attcccgaaa cgcgagctgc acaccattaa actgccgcta ccgacgcagt atcagacggc
     4681 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg ctcgcgatat
     4741 gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct ggtggaactt
     4801 cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
     4861 ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc gcgaacgtga
     4921 aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg accgcgctgc
     4981 cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag aaatcggttc
     5041 tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
     5101 ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc acgatattca
     5161 gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct ggtatcacga
     5221 aggtctggat gcatttgagc acacctgccc gaccggacgc actatttacg atagcgtata
     5281 caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat
     5341 caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacagg gtcgtgaccg
     5401 cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag aaagcattga
     5461 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
     5521 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
     5581 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg atcgtgaagt
     5641 ggcactggcg cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg
     5701 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt taaaaaacaa
     5761 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
     5821 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
     5881 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
     5941 ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg ttcacgctat
     6001 ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg atgcagcgcg
     6061 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc tgcgtgcagt
     6121 gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
     6181 ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtaacgca
     6241 tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga accctggttg
     6301 gttatcctgt atcaggatga ccacattatg gtggttaaca agccgagcgg tttgttgtct
     6361 gtgccgggtc gtctggaaga gcacaaagat agcgtgatga cgcgcattca gcgtgattat
     6421 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
     6481 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaag
     6541 cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag aaggtctggt ggatctgccg
     6601 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac gggtaaacct
     6661 gcgcagacgg aatatgaagt ggtggagtat gcggcggata acacggcaag agtggtgtta
     6721 aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct gggtcatccg
     6781 attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc acgtttgttg
     6841 ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa
     6901 gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg taggcggata aggcgttcac
     6961 gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc
     7021 ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata tgcctgctga
     7081 atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
     7141 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
     7201 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgtcgg gccacgctgc
     7261 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
     7321 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
     7381 atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
     7441 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
     7501 tggcgcatct tttcgcgcag cgggtaatta tctgattttc ccactcggaa cgcgttttgc
     7561 gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct ggcgatatga
     7621 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
     7681 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
     7741 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
     7801 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
     7861 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
     7921 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
     7981 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
     8041 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
     8101 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
     8161 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
     8221 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc
     8281 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
     8341 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga
     8401 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
     8461 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggaaggtga
     8521 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
     8581 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
     8641 ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
     8701 gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc
     8761 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
     8821 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
     8881 cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat tccgctacct
     8941 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
     9001 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
     9061 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
     9121 cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
     9181 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
     9241 tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg ttaattacta
     9301 ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc actttgttaa
     9361 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
     9421 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
     9481 gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg aatccgttaa
     9541 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
     9601 gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt acgtgccgta
     9661 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
     9721 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
     9781 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
     9841 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
     9901 tgacgacaaa acgggttcac tggtgtgggc aggcgatact tactggcgta tctccgagcg
     9961 ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag cgaccagtaa
    10021 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
    10081 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
    10141 gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc gttggtccat
    10201 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
    10261 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
    10321 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
    10381 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
    10441 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
    10501 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
    10561 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
    10621 tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct gctcaggcaa
    10681 ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
    10741 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    10801 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    10861 gtctggctta cgatggactg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    10921 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    10981 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    11041 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa
    11101 gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    11161 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    11221 aggagttgcc cgggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    11281 gcccgattcg ttccggcgtt ggcttccata ttcttaaagt taacgacctg cgcggcgaaa
    11341 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    11401 tcatgactga cgaacaggcc cgtgtgaaac tggaacagat tgctgctgat atcaagagtg
    11461 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    11521 agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc cgtgacgccc
    11581 tgactcgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    11641 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    11701 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    11761 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    11821 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    11881 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    11941 gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg cacaaccgca
    12001 aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    12061 gttagcgatt gaaaatggac attacgtggt ggagacgctg gcgcgagcgt gtgatggctg
    12121 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    12181 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    12241 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    12301 gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga ttgctatttt
    12361 gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    12421 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    12481 ggtgctcgac gagctgcggg cgcagggaat gaaactcaac gggccgctgc ctgccgatac
    12541 cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt accacgatca
    12601 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    12661 gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg gacgtggcga
    12721 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    12781 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    12841 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca aaagggccag
    12901 gcaatggtcg aaatcggccc cggtctggcg gcattgaccg agccggtcgg cgaacgtctg
    12961 gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    13021 ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa ctttggcgaa
    13081 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    13141 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    13201 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc gtatggtcga
    13261 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt tccgccgtca
    13321 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    13381 atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga agcctttaac
    13441 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    13501 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    13561 atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc
    13621 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    13681 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    13741 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    13801 aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag tacaccagcg
    13861 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    13921 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    13981 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    14041 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggtg
    14101 atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    14161 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga
    14221 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    14281 agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag aaaaagttgg
    14341 tgatggccca cgcagggatc acgccgcagt gggatctaca gaccgccaaa gagtgcgcgc
    14401 gcgatgtaga agcggtgcta tcgagtgact cctatccctt ctttctcgac gctatgtacg
    14461 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cgttttatca
    14521 ccaacgcctt tacccgtatg cgtttttgct ttccaaacgg tcaactggat atgtacagca
    14581 aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg
    14641 tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    14701 cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta acctgcctgc
    14761 gctgggaaga taagcagtat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag
    14821 cggcggcgtc ttaaacacag cctgatatag gaaggccgga taagacgcga ccggcgtcgc
    14881 atccggcact agccgtaaat tctatacaaa attaccgccg ctccagaatc tcaaagcaat
    14941 agctgtgaga gttctgcgca tcggcgtcgt ggaattcgct gaataccgat tcccagtcat
    15001 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    15061 acagtttttg cgcttttggc aggaactgct cataaacgcg accgccgcca atcaccatga
    15121 tttctggtac gtcaccacac gccgcgatgg cttcatccac cgacttcacc cacgttacgc
    15181 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    15241 cgattgattc ccaggtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa
    15301 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat
    15361 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc
    15421 cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt
    15481 tatattctgc tggcgagtcc acgctctctc cctggactcg ccgcattaca atgaaacaaa
    15541 aacaaacagt tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt
    15601 ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg
    15661 tcgttgaatt aatgacagat gttcgcgatc ctcggtaatg atctcactta acatcgcgct
    15721 ggtgcgttta tagaccgccg cgcgggcttt ggtatcattc tcaaccattg ccatctcttc
    15781 caccatctga atattaaagc ggcggaacac atcggcacgt tctcgcgctt catacggccc
    15841 cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcga aggtttcacg
    15901 ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg
    15961 ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tctgtcagtt gcaggttggt
    16021 ttgcggatcg tcgatggcgt taatcagcac ttccgctttc gccgctccgg cagattccag
    16081 taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac gcaaggtttc
    16141 gatatggtcc ggatcgtgat cgagtaccac cattttcacc ccgctggaga gcagtaaacg
    16201 tccggtaatc tgcccaaaac gaccgaatcc ggcgataatc acgcgcggct gttcttcgtc
    16261 gatctcatcg gcttcacgcg cttcctcagt agaagattgc tcaaggcgat tgaggatcac
    16321 cagcagaatc ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg atttcgccca
    16381 ctccggctcc agcacattcg ccatctgcgc cgcgccaaat accacaaagg caaactcact
    16441 gccctgccct aacaacaccg caaaccaacg acgctgttta tttggcactt gcaacggtcg
    16501 ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat
    16561 gcgcaatggg ttttcaagca gcgtgccaaa gtctatcgac atgccaacac cgatgaaaaa
    16621 cagccccaac agcaaacctt taaatggttc gatatcgctc tccagcgcat gacggtattc
    16681 cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc
    16741 ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg
    16801 caagccagag cgggctacaa aacgcagcgc cggacgcgtg acatagcgcc ccagcaatac
    16861 caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt
    16921 cgaggcactg ctcgttgcca gtagcggaat catcgccacc agcgggatcg ccgcgatatc
    16981 ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc
    17041 attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc
    17101 cgcgacctgc cagcgcagcc caagtaacat gcagaacagc cccagcaggc cgccgcaaat
    17161 caccatctgc aatgcgccac cgccgaacac tgccgcacgc agcttccaca gcctttgtgg
    17221 atcgagttcg aggccgataa taaacagcat cagcaccacg ccaatctcgg caaagtgcag
    17281 aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatga agccggcgat
    17341 caggtagcca agtaccgatc ccagaccaag acgtaccgca atgggtacaa tcagcgctgc
    17401 cgaaccgaga taaatcagcg cctgaatcag cgtatggcta tccatgatgg gcctcctgcc
    17461 attccagcag acgttgctta tagtgacgcg cctgcccttc gagggtttcg tcgtcacaaa
    17521 taaaggtgca gtgcatggca aacggtggca gccagttcag cccgcagtag attgccgtcg
    17581 cctgtagcgg ctgcgacagc acatcaaagc ccggatgcgc accaatttca aaatggcttt
    17641 ccccgccgcc ggtcgtcacc gcccacagca aatgtttgcc atgcagcgcc gtgccgccgt
    17701 gaccgtaagc ccagccgtgc gagaaaactt tatcgatcca aagtttgagg agcggtggaa
    17761 tgctgtacca ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct
    17821 gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga
    17881 cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat
    17941 acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc
    18001 cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag
    18061 agaaatctga acaatttacc ccattctctg cccgcggatc gtcatattgc tggtttgcgc
    18121 cagtgacatc cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga
    18181 gatacccgcc cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag
    18241 tgcccaggtc gaaacaatgg tgccaatacg gcttaaggac ataatcacgc ccacggcaga
    18301 ggcgcggata tctgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc
    18361 gccagagaaa aaggcataca ccgcaaaggc catcactacc agccagatcc ccatatccgg
    18421 gatcagcccc aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg
    18481 acgccgtccg gcagtgttta accacagcat cggcggaata cagccgagca taaagaacag
    18541 gctaatcacc acgttcccca gcgccgcgtt tttgccaaca cccaatccca acaaaccaac
    18601 gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc acctggcagg tccagatggc
    18661 ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg
    18721 ctgcggttgc tcttcatcga aagccaccgg ttcgccaaac agttttatca tcatctcttc
    18781 gcactctttt actcgccctt tgcgtaataa ccagcgggga gattcaggca gttcgaatcg
    18841 accaatcaaa atcaacaaac aggggatcgc cgcgctaccc agcatccagc gccagccgcc
    18901 ttccacatca taaagccagt agccgaccag atcggcgcag gtcgcgccga cataccacat
    18961 ggcggcgata aagctgatgg aaaacgcccg ctgacgggta ctggagaact cggtgatcat
    19021 tgaggtggcg atgggataat ctgcaccgat gacaatgccg ataagtaccc gcatcaccaa
    19081 cagttcgacg ggggatgaaa caaacatcgt cgccaccgat atcacgccga tggcgatgat
    19141 atcaatgagg aacattttgc gccgtccgac tttatcggaa atatagccaa acagcgatgt
    19201 gccaacgaac agcccggcga gcgttcccgc gcccaacaag ccaatccagt cagcgtccag
    19261 ttttagcgcc ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc
    19321 cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg tgaattgagg agaatttgag
    19381 atcgtcaaag tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag
    19441 tacggcgtta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    19501 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    19561 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    19621 cggggcacgc tttcaccagc agctccaggg cctgtttatc ggcatcggcc ttcacaacaa
    19681 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    19741 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    19801 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    19861 ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg tagccgctaa acatgcgtgg
    19921 gttatcaagg aaggccggta gtttctggta catacgcata tcgcgcagcg ggccactctc
    19981 aagatgctga cgatattccg ccagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    20041 aagcacggtt tttgctgcgg cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    20101 aaaaccgagg ttcatacaca ttccggcggc atcaccggca atcaatacgc cgtcaccaac
    20161 caactccggc agcatgttga tgcctgcttc cggcactacg tgagcggaat attccaccag
    20221 cttgccaccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    20281 cggcaccgat ttttttgcgt catgcagatg atgcagacca caaaccagcc ccagcgacag
    20341 ggtattttca ttggtataaa ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc
    20401 aaacagacaa gccgcgccct gattaccctg caactgaaaa cggtcttcga ttaccgactt
    20461 cggtaactcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    20521 catccccagc ttttcggcaa ggatggagtt cactccatca gcaaggatca ccgttttcgc
    20581 ttcaatcaca tcgccatcgg cttctacacc gacgactttg ccatcgcgct gtacgaggtt
    20641 atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct tcggcctgct ccatcagcca
    20701 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggcgagg tttcgtcacc
    20761 attgcagtag tccatagtca tcgctgactt ttccgtcata aacgcgagtt tttcatgggt
    20821 gatcaggcgt tctacggggg cggagtcggc gaaaccagga ataatgtgtt ccaggctgtg
    20881 ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg gaattgccac gctcgataac
    20941 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagcccagc
    21001 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    21061 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg
    21121 atcttcacgg cgtcaccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    21181 tctttattga tggcgaaaat ggtttgcgat gcattagcac caaccatgtg ctggatctgc
    21241 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    21301 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    21361 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    21421 cgaccgacgc tgaccaccag acgggctttg tcgagatcga cgctgttgct ctggcgcgcc
    21481 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    21541 ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc
    21601 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac caccgtaaac catgtgtttc
    21661 actgtcgctt taccgtcctg tacgctgacg gtgctggcat cgttagacac cgccgcttta
    21721 aggcgataac ccagttttgc cgccagtaat ttgccgcgac gggtgtttgg cagcagcacc
    21781 aggccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    21841 atccgatcgt ccggtttgcc gtttaatttc cagacatgat tagcgccgag ctggattgcc
    21901 tgtacgccgt cggcatcatt gaggacaaag gtgttgattt gattagctaa agcctgcgca
    21961 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    22021 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    22081 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ttcgcgctgt ttcggcgcgg
    22141 caacctgttg ttctgaccag gctgcctctg cgttaaaacc aatatccgcc gccgaccata
    22201 cctggacggg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    22261 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tcggtttcat
    22321 cttccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    22381 cgttaactgc cggaatattg aggatttcgc ccaccagcag accaacctgc tgggcataaa
    22441 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctgg gcggctgcag
    22501 ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    22561 tcagttcatc cgggccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    22621 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    22681 ccgcttcaat agcgttcaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    22741 atgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    22801 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    22861 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    22921 tgtcacacgc agataaatcc aactttcaat attgttaagt tcctcaccaa tattgaaaac
    22981 atggcgtagc aaaaagaaat tttcaatatt gctttatgga tcaccaatat tgaaagtcag
    23041 atgctatttt tttactcaca acacaacata acaaactgat taataatcaa aattaacgag
    23101 aaaagcgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa tgcagcttat
    23161 tgaataccca ttatgagtta gccattaacg cgtccacgag gttaataata attatattaa
    23221 atgttaacaa aaataaaaca aacgggaacg caaaataaat attcgttttc acagtggaat
    23281 taactcatga agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg
    23341 ttaataatcg tcggcatact ttgttggctt acagtcagag atctggatgc agcgaatgtc
    23401 gttattaatg ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat
    23461 atggtggtga tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt
    23521 ttaggtaacg aaccacctga atttagtacc gccagttgga tctttatgat gttcgcctcc
    23581 tgtacgtctg ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc
    23641 ccgccgtttg gcttagaacc gaactcgaca ggggcgaaag agttggggct ggcttacagc
    23701 ttgttccact ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct
    23761 tacttcttct ttgtccgcaa aatggaagtg attcgcccca gctcgacact ggtgccgctg
    23821 gtaggtgaaa aacacgccaa agggttgttc ggcactatcg tcgacaactt ctatctcgtc
    23881 gccttgatct tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt
    23941 atgcaatggt tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc
    24001 tggattatcc tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc
    24061 agtgacgtgc gtagttacct gagcttcctg atgctgggtt gggtgttcat tgtcagcggt
    24121 gccagcttca tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg
    24181 cgcatgttgt tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg
    24241 ttctactggg catggtgggt gatttatgct atccagatga gtatcttcct cgcccgcatc
    24301 tcccgtggtc gtactgtgcg tgaactgtgc ttcggcatgg tgctggggct gacagcgtca
    24361 acctggatcc tgtggactgt actcggttgt aacactctgc tgttgataga taaaaacatt
    24421 atcaacattc caaatctgat cgaacagtac ggtgtggcgc gcgccattat cgaaacctgg
    24481 gccgctctgc cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc
    24541 accgttacgc tggttaacgc ctgctcttat accctggcga tgtccacttg ccgcgaagta
    24601 cgcgatggtg aagaaccacc gctgctggtg cgtatcggtt ggtcaattct ggttggcatt
    24661 atcggtattg ttttgctggc gctcggcggc ctgaaaccga ttcaaactgc cattatcgcc
    24721 ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg
    24781 aaacagaact ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt
    24841 aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa
    24901 aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc
    24961 aaagcactgg cggatatggg tatcgacagt ctgctgatcc ctgaagagca cggtggtctg
    25021 gacgcggggt ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca
    25081 acctatgtac tgtaccagtt gccgggcggg ttcaacacct tcctgcgcga aggcacacaa
    25141 gagcagatcg acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg
    25201 attaccgaac cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga
    25261 aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagcagcgc ctacaccccg
    25321 tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg
    25381 tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt
    25441 atggatagct gctgtgaaat cacctttgac gacgtggaac tggacgagaa agacatgttc
    25501 ggtcgggaag gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg
    25561 gtagccctga ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc
    25621 aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc
    25681 gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa
    25741 gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat
    25801 gcggcatttg aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc
    25861 aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag accgcgtctc cgggggatct
    25921 gacgaaatgc agatcctgac gctgggtcgt gcggtgctga agcaataccg ctaagtcaat
    25981 gcgctgcctg atgcgacgct gatgcgtctt atcaggccta cgggagcacg aatgtaggcc
    26041 ggataaagcg tttacgccgc atccggcagt catgcgctac ataaattttt caggagcaaa
    26101 taatggatca tctacccatg ccgaaattcg ggccgttggc cggattgcgc gttgtcttct
    26161 ccggtatcga aatcgccgga ccgtttgccg ggcaaatgtt cgcagaatgg ggcgcggaag
    26221 ttatctggat cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc
    26281 aactctcccg ccgcaatttg cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg
    26341 aagcgtttct gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg
    26401 cctttgcccg tcgtggcatt accgatgaag tactgtggca gcacaacccg aaactggtta
    26461 tcgctcacct gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct
    26521 ataacactat cgcccaggcc tttagtggtt acctgattca gaacggtgat gttgaccagc
    26581 caatgcctgc cttcccgtat accgccgatt acttttctgg cctgaccgcc accacggcgg
    26641 cgctggcagc actgcataaa gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca
    26701 tgtatgaagt gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg
    26761 aaatgtgccc gcgcatgagc aaaggtaaag atccctacta cgccggttgc ggtctgtata
    26821 aatgcgccga cggctacatc gtgatggaac tggtgggcat tacccaaatt gaagagtgct
    26881 ttaaagatat tggcctcgca catctactcg gtacgccgga aatcccggaa ggcactcagc
    26941 ttatccaccg tatcgaatgc ccttacggcc cactggtaga agaaaaactc gatgcctggc
    27001 tggcggcaca taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg
    27061 ccaaagtact gaccgtaccg gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa
    27121 tcactcagtg gcaaacgatg gatggtcgca cctgcaaagg gccgaacatc atgccgaaat
    27181 tcaaaaataa ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg
    27241 ccattttgaa aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc
    27301 tggccaaagt tgaggactaa acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc
    27361 aataacaaac gaacaaaaat ggatagaggt gcaatggata tcattggcgg acaacatcta
    27421 cgtcaaatgt gggacgatct tgcggacgtt tacggtcata aaacggcgct gatttgtgaa
    27481 tccagcggcg gagtcgttaa ccggtatagt tatcttgagt taaatcagga gattaaccgc
    27541 acggcaaacc tgttttatac gctggggatt cgcaaaggcg acaaggttgc actacatctc
    27601 gacaactgcc cggaatttat cttttgctgg ttcgggctgg caaaaattgg cgcgattatg
    27661 gtgccgatta acgcccgcct gttacgcgag gaaagcgcgt ggatcctgca aaatagccag
    27721 gcgtgcctgc tggtgaccag tgcgcaattc tatcctatgt atcaacagat tcagcaggaa
    27781 gatgccactc aattgcggca catttgcctg acagatgtgg cacttcccgc tgatgatggc
    27841 gtgagttcgt ttactcaact gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg
    27901 ctatcgactg acgatacggc ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa
    27961 ggtgtggtga ttacccatta caacctgcgc ttcgctggat attactccgc ctggcagtgt
    28021 gcactgcgtg acgatgacgt ctacctgacg gtaatgcctg cgtttcatat cgattgccag
    28081 tgtactgcgg cgatggcggc gttttctgcc ggggccacct ttgtgctggt cgagaaatac
    28141 agcgcccgcg ccttctgggg acaggtgcag aagtaccgcg ccaccattac cgaatgtatt
    28201 ccgatgatga tccgtacgtt gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc
    28261 ctgcgggaag tgatgtttta tctcaacttg tcggagcagg aaaaagatgc gttttgtgaa
    28321 cgcttcggcg ttcgcttgct gacgtcttat gggatgacgg aaaccattgt gggcattatc
    28381 ggcgatcgcc ctggcgataa acgacgctgg ccgtcgattg gtcgggcggg gttttgctac
    28441 gaagcggaga tccgcgacga tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc
    28501 tgtattaaag gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc
    28561 actgcgaaag tgctggaagc cgacggctgg ctacataccg gcgataccgg atactgcgac
    28621 gaagagggct ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg cggtggcgag
    28681 aatgtctcct gcgtggagct ggaaaatatc atcgccaccc atccaaaaat tcaggatatc
    28741 gtggttgtgg gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg
    28801 aatgaaggtg aaacattgag cgaagaggaa tttttccgct tctgcgaaca aaatatggcg
    28861 aaatttaaag tgccctctta tctggagatc agaaaagatc tgccacgtaa ttgctcgggg
    28921 aaaataatta gaaagaatct gaaataaatg aaacagcagg gaactaccct gccagctaat
    28981 aatcacgcta taaaacaata tgcatttttt gcagggatgt tgtcgtccct gaaaaagcaa
    29041 aaatggagaa aaggaatgag tgaatcatta catctgaccc gcaatggatc aattctggaa
    29101 attacccttg atcgtccaaa agcgaatgct attgatgcaa aaaccagctt tgaaatgggc
    29161 gaagtatttc taaatttccg tgacgatccg caattacgtg tcgccattat taccggtgcc
    29221 ggagagaagt tcttttccgc gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg
    29281 gatgctgact ttggcccggg tggatttgcg ggattaaccg aaattttcaa tctcgacaaa
    29341 ccggttatcg cagctgtgaa cggctatgcc tttggcggcg gctttgaact ggcgctggcg
    29401 gcagatttta ttgtttgtgc cgataatgcc agcttcgccc tgccggaagc caaactgggc
    29461 atcgttcctg acagcggcgg cgtgctgcgt ctgccgaaga tcctgccgcc tgccatcgtc
    29521 aatgaaatgg tgatgaccgg cagacgaatg ggcacagaag aggcgctgcg ttgggggata
    29581 gtcaaccgcg tggttagcca ggcggaactg atggataacg cccgcgaact ggcgcagcag
    29641 ctggttaaca gcgccccgct ggcgattgcg gcgctgaaag agatctaccg caccaccagc
    29701 gaaatgccgg tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa acactatccg
    29761 tcggttctgc attcggaaga tgccgttgaa gggccgctgg cgtttgccga gaagcgcgat
    29821 ccggtatgga aaggacgtta acaaccgtga gctattacgc ctttgagggg ttaattccgg
    29881 tggttcaccc gacggcgttt gtccatccca gtgccgtctt gattggcgat gtgattgtgg
    29941 gagccggtgt ctacatcggc ccactcgcct cactgcgtgg tgactacggg cggttgatcg
    30001 tgcaagcggg agccaatatt caggatggct gcattatgca tggctactgc gacactgaca
    30061 ccatcgttgg ggaaaacggc catatcgggc acggagcgat cctgcatggt tgtgtgattg
    30121 gtcgcgatgc attggtcggg atgaacagcg tgattatgga tggcgcggtc attggcgaag
    30181 agagcattgt tgccgccatg agctttgtca aagcaggctt tcacggcgag aaacgccagt
    30241 tgttgatggg tacgccagcc cgcgctgtac gcagtgttag tgacgacgag ttacactgga
    30301 aacggttgaa taccaaagag tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg
    30361 aaacacagcc actgaggcaa atggaggaaa atcgcccccg tttgcagggg acgacggatg
    30421 tgacgcctaa acggtgatct tgccggatgc gacctggttg ctggataaga tgctacaaca
    30481 tcgcatctgg caagcaggac gcccggtatc aacgagataa aattaacgac gcatactctt
    30541 tgacaacatc atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat
    30601 acgcggcgtc tttcttacgc cgactaattt atcccggctg ttattgcgca ggcgcgcgta
    30661 aatttgctca tcgatatcga ccacttttac cagtcgctgg cactggcatc cccgtccttc
    30721 cagcttatta gggatcatct taacttcgca gcttatttct gcgacttccg acagaatata
    30781 agtcagggta ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc
    30841 tatcacccac cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc
    30901 aacatatcct tcacacatac gaactccagt ttcacaaatt aaaagtctgg ctatgactcg
    30961 ttttgaagca attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt
    31021 caccgtcgat aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa
    31081 agatttactt aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta cttattcgat
    31141 caaattagcg gatgaaaaat atccgccatg acacgctatt atttgatctg cgcgtgcatt
    31201 tcctgcaccg aaattacttt ttcggtcgcg tcggcattca gcgccatcgc ggtagcgaaa
    31261 ccaccgttca gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg
    31321 cgggagtctt caatcgcgcg acggcctgaa gtggtgttga tgatgtaggt atattcgcca
    31381 ttcttgatgc ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga
    31441 ttaatgcccg cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc
    31501 tgtttcagca gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa
    31561 agcaacgcac gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac
    31621 gcttcagcga aggtgcggcc cacgcccatc acttccccgg tggagcgcat ttctggccct
    31681 aacagcgggt caacacccgg gaatttattg aacggcagca ccacttcttt caccgagtag
    31741 tacggcggga taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca
    31801 cgcgccgcca cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc
    31861 gccgcacgcg ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg
    31921 ttcatcagac cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca
    31981 tcctgaattt cctgacttaa ggtgtaggct ggcagagaac atgcggagtc accggagtgc
    32041 acgcccgcct gctcgatgtg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag
    32101 atggcatcca cgtcaacttc taccgcgtca tcaaggaagt gatccagcaa cactggcgca
    32161 tcgttagaca cgctgaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg
    32221 atttccatcg cccgaccgcc gagaacgtaa gacggacgta ccaccagcgg gtagccaatc
    32281 tctttcgcct tctctaccgc catttcaata gcggtaacgg tggcgttcgc cggttgtttc
    32341 agtttcagac gctcaaccgc atgctggaag cgttcacggt cttctgcacg gtcgatagca
    32401 tccgggctgg tgccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc
    32461 ggggtctgac cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttct
    32521 agcacatctt ccagagttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggtg
    32581 gagacggttt ccgggttaca gttaaccata atggtttcgt aaccgtcttc gcgcagcgcc
    32641 agcgaggcgt gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg
    32701 ccgccgagaa ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca
    32761 taagtggagt acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc
    32821 ttataaaccg ggtgcaggtc atactggtcg cgcagcttgc ggatttccgc ttcgcgcacg
    32881 cccgccagtt ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat
    32941 tcagcgttca ggccagtgat gcccacttcc gctactttct cttccagacg caccagttct
    33001 tcaatctgta ccaggaacca gcggtcaata ttggtcaggt tgaacacgcc gtccacagac
    33061 aggcccgcac ggaaggcatc ggcgatgtac cagatacgct ctgcgccagc atctttcagt
    33121 tcgcgacgga ttttggttaa cgcttccggg tcatcgaggc tcactttcgg gtcgaatcca
    33181 gtcgcaccga cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca
    33241 atcgccatca cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccagcg
    33301 aatttttcga agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag
    33361 gaggccggag tacgtccgcc agtgatgtcg ttcatcagtt cgtcgagggt gtaacccacc
    33421 gccagtttcg ccgccacttt agcaatcggg aaaccggtcg ctttcgacgc cagcgccgaa
    33481 gaacgggaca cgcgtgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc
    33541 gcaaactgga cattggaacc accggtttca acgccgattt cacgcagcac cgccatcgag
    33601 gcgttacgca tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg
    33661 gagtcaccgg tatggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag
    33721 ttgtcgtttt tatcacgcac cacttccatc tcgtactctt tccagccgat cagcgactca
    33781 tcaatcagca gctctttggt tggggagaga tccagaccgc gggcgcaaat ttcttcaaac
    33841 tcttcgcggt tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata
    33901 atgcacggga agcccacgtc agcggcaacc gccagcgctt cttccatcgt gtgtgcgata
    33961 ccggaacgcg cggtttccag accaattttc ttcatcgcta cgtcgaaacg acggcggtct
    34021 tctgctttat caatcgcatc ggcggtcgca ccaatcatag tcacgccgaa ctcttccaac
    34081 acgccctgac gctccagctc cagcgcgcag ttcagcgccg tctgaccgcc catcgttggc
    34141 agcaccgcgt ccgggcgctc tttttcaata atcttgcgta ctacttccca gtgaatcggc
    34201 tcgatgtagg tcgcatcggc catttccggg tcggtcatga tggtcgccgg gttggagttc
    34261 accagaatga cgcggtaacc ctcttcgcgc agggctttac atgcttgcgc gccagagtag
    34321 tcaaactcac acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt
    34381 atatctgtac gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact
    34441 gctcaattaa ctcgataaag tggtcgaaca acggcgctgc gtcgtgtggc ccagggctgg
    34501 cttccgggtg accctggaag ctgaacgccg gtttatcagt gcgatgaatg ccctgtaacg
    34561 taccgtcgaa cagggattta tgcgtgacac gcaggtttgc aggtaatgtt gcttcatcca
    34621 ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt tttctccaca tctttaaccg
    34681 gatggttgcc gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg
    34741 ccagcagctg atgaccgagg cagatgccga ataccggaat atcggtttcg aggaatttct
    34801 ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga
    34861 tgccgtctgg attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca
    34921 ggcgacagcc tctatccacc agcatccgca ggatgttgcg cttggcacca aaatcataag
    34981 ccacgacgtg gaacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcaacg
    35041 tccagctccc ttgtgtccag ctataggctt ctgcggtggt cacttctttt gccagatcca
    35101 tgccattcag acctgggaac gcgcgggctt tttctaacgc caacgccgca tccgggttat
    35161 cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgc gtcagcttac
    35221 gggtatcgat atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt
    35281 cggtattacg gaagttgctg gcaatcagcg gcagatcgcg aatcaccaga ccttgtgcat
    35341 gtacctgaga agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag
    35401 taagagtaac gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca
    35461 ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt
    35521 gaaactgggt tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag
    35581 aatattcact cactttattt gcatattaat tcacaatgat gacgtaaatc aatgcaaatc
    35641 tgcttaccga cgaatttctg gcaaacggcg gcattctgga gatatgggcg ctaaaagtca
    35701 acttaatggc gggatttttt atattatttt gtggctcatg catgttttac aggcattaag
    35761 cgacaaaaag atcatataac ctgttctgga aaacgcttgc gcagcaagaa gattgcatga
    35821 aataaaagcg agcagggaaa agtggaccaa atggtcaaaa tttacattaa aacaacaaaa
    35881 accgaagaca aaacatgtaa ttttt
//
LOCUS       DAZGCS010000047        35088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGCS010000047 DAZGCS010000000
VERSION     DAZGCS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.047"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHK01_002361"
     CDS             complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHK01_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO8097307.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHK01_002362"
     CDS             1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHK01_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO8097308.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHK01_002363"
     CDS             complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHK01_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO8097309.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3972..5087
                     /gene="macA"
                     /locus_tag="ACHK01_002364"
     CDS             3972..5087
                     /gene="macA"
                     /locus_tag="ACHK01_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO8097310.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5084..7030
                     /gene="macB"
                     /locus_tag="ACHK01_002365"
     CDS             5084..7030
                     /gene="macB"
                     /locus_tag="ACHK01_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO8097311.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVIERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHK01_002366"
     CDS             complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHK01_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO8097312.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7477..7551
                     /gene="yljB"
                     /locus_tag="ACHK01_002367"
     CDS             7477..7551
                     /gene="yljB"
                     /locus_tag="ACHK01_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO8097313.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7732..8955
                     /locus_tag="ACHK01_002368"
     CDS             7732..8955
                     /locus_tag="ACHK01_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8097314.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLDV
                     LAGNHENIVMLKVAK"
     gene            8952..9725
                     /locus_tag="ACHK01_002369"
     CDS             8952..9725
                     /locus_tag="ACHK01_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097315.1"
                     /translation="MKLPKRLYYPLPEAAEKLGCAIKDLYHYAYIGALNISVYIPKID
                     AKKFNFSLCHFINGCNEEELIKYPFNDESAVFGSNFTVGLTGDTEFLDSAIIGPVGNS
                     IKCYYVSSLSGFYYTPAYVFADVEFSMRQYIKCSHLTNKYGDDNLMYVFCKGGYEFDV
                     NYLCVMASDLDNIKNTGDFPKITEDSPKASSKRNEVIYSLIKLIPEMGDVDLDTESLP
                     KIADLIDSIAASRGIEFPKTHWQTWQKYLGRESQQKRKK"
     gene            9817..10041
                     /locus_tag="ACHK01_002370"
     CDS             9817..10041
                     /locus_tag="ACHK01_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8097316.1"
                     /translation="MNQNTQFQSLSDRVIREDECRKLTGVCRTTRYELEKKGNFPSRR
                     NLGGRSVGWLLSEVMEWVKSRDRINSGKAA"
     gene            complement(10176..10355)
                     /locus_tag="ACHK01_002371"
     CDS             complement(10176..10355)
                     /locus_tag="ACHK01_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8097317.1"
                     /translation="MATKAVNGKSKKLEARVPHAIADAVENLKEEGESTGQFIVSALE
                     GEIKRRQRRKAKEQE"
     gene            10600..10758
                     /locus_tag="ACHK01_002372"
     CDS             10600..10758
                     /locus_tag="ACHK01_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO8097318.1"
                     /translation="MNKYIWRFLALSAGESQIIHVTAWTEREARSRCPSGCVAVFVAR
                     IRQGVCRA"
     gene            10751..10924
                     /locus_tag="ACHK01_002373"
                     /pseudo
     CDS             10751..10924
                     /locus_tag="ACHK01_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10937..11128
                     /locus_tag="ACHK01_002374"
     CDS             10937..11128
                     /locus_tag="ACHK01_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097319.1"
                     /translation="MKEITLHEAAERAHQTEIICRLLEVYPNKLNDGDISALAGLLAK
                     LSGSVALWLIDERFKRYEQ"
     gene            11118..11360
                     /locus_tag="ACHK01_002375"
     CDS             11118..11360
                     /locus_tag="ACHK01_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097320.1"
                     /translation="MNNNKRVICELNEFTQSAISYNNAVSEIINVWFESLTNCERDES
                     TTRLTFAISELLNISTSKIESISEKINGLSGHAIGE"
     gene            11366..11665
                     /locus_tag="ACHK01_002376"
     CDS             11366..11665
                     /locus_tag="ACHK01_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8097321.1"
                     /translation="MKLKYSGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFNRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            11662..13410
                     /locus_tag="ACHK01_002377"
     CDS             11662..13410
                     /locus_tag="ACHK01_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFO8097322.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELYALQIVPDGKKRVRLYQAGELDLVETKKIGQKLAAAGVQDA
                     KFYPEGMHGQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLADRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDKDLPEKVAAEIP
                     VIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVADFSKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDDCDAFL"
     gene            13762..14007
                     /locus_tag="ACHK01_002378"
     CDS             13762..14007
                     /locus_tag="ACHK01_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097323.1"
                     /translation="MTKMRRDRTQQKYKALDMTELSLKVAIKVIDRHTREGYAKENPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSTGE"
     gene            14138..14332
                     /locus_tag="ACHK01_002379"
     CDS             14138..14332
                     /locus_tag="ACHK01_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097324.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDVIRRMNEAGIYGRAATPT
                     IGHLARVLLRERLGIATGEA"
     gene            14336..14497
                     /locus_tag="ACHK01_002380"
     CDS             14336..14497
                     /locus_tag="ACHK01_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO8097325.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSNEVE
                     GLISLLGEQ"
     gene            14627..15115
                     /locus_tag="ACHK01_002381"
     CDS             14627..15115
                     /locus_tag="ACHK01_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8097326.1"
                     /translation="MALTTKKKRFADALSRGANPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            15127..15288
                     /locus_tag="ACHK01_002382"
     CDS             15127..15288
                     /locus_tag="ACHK01_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097327.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            15278..16201
                     /locus_tag="ACHK01_002383"
     CDS             15278..16201
                     /locus_tag="ACHK01_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8097328.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEMTKRNDPAVSKFLE
                     KSIVNRMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLHSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIEIFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            17180..17500
                     /gene="clpS"
                     /locus_tag="ACHK01_002384"
     CDS             17180..17500
                     /gene="clpS"
                     /locus_tag="ACHK01_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO8097329.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            17531..19807
                     /gene="clpA"
                     /locus_tag="ACHK01_002385"
     CDS             17531..19807
                     /gene="clpA"
                     /locus_tag="ACHK01_002385"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO8097330.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVQLAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(20151..20238)
                     /locus_tag="ACHK01_002386"
     tRNA            complement(20151..20238)
                     /locus_tag="ACHK01_002386"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20202..20204),aa:Ser,seq:gga)
     gene            complement(20492..20710)
                     /gene="infA"
                     /locus_tag="ACHK01_002387"
     CDS             complement(20492..20710)
                     /gene="infA"
                     /locus_tag="ACHK01_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO8097331.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(20995..21699)
                     /gene="aat"
                     /locus_tag="ACHK01_002388"
     CDS             complement(20995..21699)
                     /gene="aat"
                     /locus_tag="ACHK01_002388"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO8097332.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCSESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(21741..23462)
                     /gene="cydC"
                     /locus_tag="ACHK01_002389"
     CDS             complement(21741..23462)
                     /gene="cydC"
                     /locus_tag="ACHK01_002389"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO8097333.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(23463..25229)
                     /gene="cydD"
                     /locus_tag="ACHK01_002390"
     CDS             complement(23463..25229)
                     /gene="cydD"
                     /locus_tag="ACHK01_002390"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO8097334.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(25352..26317)
                     /gene="trxB"
                     /locus_tag="ACHK01_002391"
     CDS             complement(25352..26317)
                     /gene="trxB"
                     /locus_tag="ACHK01_002391"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO8097335.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            26862..27356
                     /gene="lrp"
                     /locus_tag="ACHK01_002392"
     CDS             26862..27356
                     /gene="lrp"
                     /locus_tag="ACHK01_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO8097336.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            27491..31558
                     /gene="ftsK"
                     /locus_tag="ACHK01_002393"
     CDS             27491..31558
                     /gene="ftsK"
                     /locus_tag="ACHK01_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO8097337.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            31713..32324
                     /gene="lolA"
                     /locus_tag="ACHK01_002394"
     CDS             31713..32324
                     /gene="lolA"
                     /locus_tag="ACHK01_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO8097338.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            32335..33678
                     /gene="rarA"
                     /locus_tag="ACHK01_002395"
                     /gene_synonym="mgsA"
     CDS             32335..33678
                     /gene="rarA"
                     /locus_tag="ACHK01_002395"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO8097339.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            33769..35061
                     /gene="serS"
                     /locus_tag="ACHK01_002396"
     CDS             33769..35061
                     /gene="serS"
                     /locus_tag="ACHK01_002396"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO8097340.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         9310 a         8394 c         9214 g         8170 t
ORIGIN      
        1 tcatgaaagg agcgcaacaa gatgatgaat caacatataa caacatctta aaaaaaggcc
       61 tgacattacg ccaggccttc tgcgttaatt aatcacgctt ttccagcagg gtccggtaaa
      121 tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta
      181 atgcccagcc gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt
      241 tagtcaccgg aatactaatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg
      301 caaaacctgc cggcgcgaat ttgtcggttg cgccgtggat caccaacagg aaacctgcac
      361 tcaataccag ttcaactacc agcgcggaaa gcatggaata accgcctggt gaatgctcgc
      421 cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa
      481 ttaaatacag cagcgccgct gcaacaatac cgccgacaac ctgggcaatt acgtagccaa
      541 cgacttcttt tgccggaaaa cgtccgccag cccataaacc aatagtgacc gccgggttaa
      601 aatgaccacc agaaatatga ccaacagcaa aggccatcgt cagaacggtc agaccgaacg
      661 ccaacgccac gccggcaaaa ccaatgccta attccgggaa gcctgcggcc agtacagcac
      721 taccacagcc accaaaaaca agccagaaag taccaaaaca ttcagctgct aattttctga
      781 acatatccac ctcaattaaa aattgaccct gtgaaaaata tggtcgtttt atagggtcgt
      841 cgtaaaaaag tgacgacgga aataatgcgc ggctatttta aaaacgaagg cgagtcattc
      901 accagataaa taaatccagt aaatttgatt tagggcaaca gcgggttgcc ccatatagtc
      961 atttgtctga ttgacagtgt agtgcaagca aaagatttaa tcctttaggc gtaataaaaa
     1021 ataatttatc atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga
     1081 gtataacgcg attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc
     1141 acgtattagc aaggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct
     1201 ggaggggata tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg
     1261 tcgttgatgc tggaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc
     1321 ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc
     1381 gacgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt
     1441 ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa
     1501 gcgtgctgga cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag
     1561 agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg
     1621 attgatgtcg aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg
     1681 cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc
     1741 aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg
     1801 caaaatctct ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag
     1861 cattatttct ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc
     1921 agcaatgagc aacgaagctg gcgctatctg gatatcatca accggatgat tgaccgacct
     1981 ggagggcgct cgtatcgggt tattttgctc ggcctgtttg caactttatt gcaggcaaaa
     2041 ggcacattgc gactggataa agacgcccgt ccattgttgc tgattgaaga tccagaaacc
     2101 cgtttacacc ccattatgct ttcagttgcc tggcatctgt tgaatcttct gccattgcag
     2161 cgcattgcca ccaccaactc gggtgagttg ctttcgttaa ctccagtgga acatgtttgc
     2221 cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc
     2281 accgaagaca gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt
     2341 gctcgctgct ggttgctggt ggaaggggaa acagaaacct gggttatcaa cgaactggcg
     2401 cgccagtgtg gacaccattt cgatgccgaa gggatcaagg tcattgagtt tgcccagtcc
     2461 gggctaaagc cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc
     2521 gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat
     2581 cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg
     2641 tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg
     2701 atgaatctgc gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc
     2761 attgaagtgg caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa
     2821 aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc
     2881 gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt
     2941 tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg
     3001 cgttcagcat cacatacgcc gccaaccgat tcccagaaag cgttgtaatc ggcatggatc
     3061 ttgccttctt tatcacgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca
     3121 ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt
     3181 ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc
     3241 cattttgcgc cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg
     3301 gtaaacgtga tctctgccag aggaatacct tcgctgttgc gaaacaggat tgtgctgtca
     3361 ccttctttat ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg
     3421 ccttccagct tcgccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc
     3481 gacatacatc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg
     3541 ctaagattcg ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt
     3601 aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc
     3661 ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta
     3721 cccggccgac actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca
     3781 gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat
     3841 gacgtcgatt tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa
     3901 attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat
     3961 tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga
     4021 tagtcatcgc cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc
     4081 agacactgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg
     4141 acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa actctgtcgg
     4201 tggcgattgg cgataaagta aaaaaagacc agcttttagg ggttattgat cctgaacagg
     4261 ctgaaaacca gattaaggag gtcgaagcaa cgctgatgga gctacgtgcg cagcggcagc
     4321 aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac
     4381 aaacgcaggc ggtttcacag caggatctcg acaccgccgc gacggagatg gctgtgaaac
     4441 aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcttct ctcgatacgg
     4501 ctaaaaccaa tctcgattac actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa
     4561 tcaccactct gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc
     4621 tggcagatat gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc
     4681 tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg
     4741 ggcaaatcaa ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt ttctattacg
     4801 cccgttttga agtccccaac cccaatggtt tgctgcggct ggatatgact gcgcaagtgc
     4861 atattcagct caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc
     4921 cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg
     4981 aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg cttgaagcgg
     5041 gcgatgaagt ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga
     5101 attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg
     5161 catcaccctc gatatttatg ccggtgagat ggtagcgatt gttggcgctt caggttccgg
     5221 taaatcgacc ctgatgaata ttctcggctg tctggataag gccaccagcg gcacctatcg
     5281 cgtcgccggt caggatgttg ccacactgga cgccgatgcg ctggcgcaac tgcgccgcga
     5341 gcatttcggc tttattttcc agcgttacca tttactttcg catttaaccg ccgagcagaa
     5401 cgttgaagta cccgccgtct atgccggtct tgagcggaaa cagcggctgc ttcgcgccca
     5461 ggggttactg caacgactgg ggctggaaga ccgtacagag tattatccgg cacagctttc
     5521 gggtggtcag caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat
     5581 tcttgccgat gaaccaaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat
     5641 cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt
     5701 cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc
     5761 cgccattgaa aaagtgaatg ttgctggcgg gacggagcct gttgtcaaca cggtgtctgg
     5821 ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc
     5881 agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt
     5941 ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc
     6001 tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca
     6061 atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc
     6121 ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc
     6181 tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga
     6241 aggaaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag
     6301 taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt
     6361 cggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag
     6421 cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca
     6481 gtcgtggctg aactccatta ctgtcagggt aaaagaagga tttgacagcg ccgagacgga
     6541 acagcaactc acgcgtttac tttcactgcg ccacggaaag aaggatttct ttacctggaa
     6601 catggacggc gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac tgtttctgac
     6661 gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt
     6721 gtcagtgatc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag
     6781 cgatgttttg caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt
     6841 gggaataact ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga
     6901 gattggtttt tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg ccaccgggat
     6961 tttatttgga tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc
     7021 acgagagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga
     7081 tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa
     7141 cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt
     7201 ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc
     7261 cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct
     7321 tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc
     7381 agagaggggg ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg
     7441 agccgtcaag cgtttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt
     7501 aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttattt
     7561 atcagttagt gttcgtgggt gttcgtcatg gtgtgggcat ctaatttatt tctatgatta
     7621 taaaggataa tttcgttttt cttgttcgcc cttgttcgtc acagttcgcc cttgtacgat
     7681 gcacggtgta gtcaatggtg tagttaattt tgatgaccgg aaacacgaaa catgagcagg
     7741 gcaattcata gacttagcga cactctttta cgaaaattaa gcggatcacc aaccacaaaa
     7801 agcacctttt ttaatgatgg tggcaacctt agcgtaaggc attcaaccaa tggcctgtta
     7861 acctggtatt tcacttacag gtccggaact ggtaggcaga tatcacctga acgtctgagg
     7921 ctgggaaatt atccagattt gagcctgaaa gcagccaggg aaaaagcgtc gcaatgtcgc
     7981 gcatggctgg ctgaggggaa aaatccgcgc tatgaactta atcatgctgt acaggacgcg
     8041 ttagcccccg tgacggttaa ggaagcgctc acttactggc ttgaatcgta cgcaaaggaa
     8101 aagcgcactg actacgaatc actgaaaagc cggatcaata aacacataat tagccagatt
     8161 ggagctatgc cactggaaaa atgcgagcta cgccactggc tggcctgttt tgaccagctt
     8221 gctaagcgta atccggtatc agccggattt ttgctacagg tatgcaaaca ggcgcttaag
     8281 tactgccgaa aacgacgata cgcaatcagc aacgttcttg atgatatggt tgtcggtgat
     8341 gttgggaaaa aagcagaaat aagcgagcgc gtactaacaa acaaggagct tggggaatta
     8401 ctacgcgccc ttgatgaaaa aatttaccca ccgtactaca gcgccctgat tcgcctcctg
     8461 attgtcttcg gatgtcgtac cactgagcta agacgctctg aggtccagga atgggatttt
     8521 aaagaaatgc tctggaccgt gccgaaagaa cacagcaaaa cgaaggtagc aatattcagg
     8581 ccaataccgg aagcgatctt gccgtttgtc atgaagctgg tggagcagaa caggcacacg
     8641 ggcttattgc tcggagaact gaaagggcaa tcatccgtat cagagtacgg aagaacggca
     8701 cacagacgca ttaatcaagc cccctggacg ttgcacgaca tccggcacac gtttacaacg
     8761 atgctgaacg atttaggcgt ggaaccgcat gttgtggagc agcttaccgc gcaccagttg
     8821 ccaggaatgc aacgagtcta taatcactcc cgttatcttg atgcaaaacg tgacgctctt
     8881 aatctatggg ttgagcgcct cgatgtgctg gctggaaatc atgaaaatat tgttatgctg
     8941 aaggtggcca aatgaaatta cctaaaagac tctattatcc gttacctgaa gcggcagaaa
     9001 agttaggctg tgcaataaaa gatctttatc attacgcata tataggtgct cttaatattt
     9061 ctgtatatat accaaaaatt gatgctaaaa aattcaattt ttcattatgt cattttatca
     9121 atggttgtaa tgaggaagaa ttaataaaat atcctttcaa tgatgaatct gctgtttttg
     9181 gaagtaattt tacagttggt ttaactggtg acactgaatt tttagatagt gccattattg
     9241 gtcctgtagg gaattctatc aaatgttatt atgttagcag cttatcaggg ttttattata
     9301 ctccggcata tgtttttgct gatgtcgaat ttagcatgag acaatatata aaatgttctc
     9361 atcttacgaa taagtatggt gatgataatc tcatgtatgt tttttgtaag ggtggttatg
     9421 aatttgatgt taactatttg tgtgtgatgg ctagtgattt agataatata aaaaatacgg
     9481 gagacttccc gaagataaca gaagatagcc caaaagcatc gtcaaaaagg aatgaggtta
     9541 tttactcttt aattaaactt attccagaaa tgggtgatgt agacctggac acggaatcat
     9601 tacctaaaat agcagattta attgattcta tagctgcatc cagagggata gaatttccaa
     9661 aaacacattg gcagacatgg caaaagtatt tggggcgtga atctcaacaa aaacgtaaaa
     9721 agtgatatca cgtgctggta gtgtgatatc acacaaacgg cgttttacta ccgcctagaa
     9781 tcgtcctcgt tcggcaacgt tcgaggacgt ttttttatga atcaaaatac acaatttcaa
     9841 tcactatcag atcgcgtaat ccgtgaggat gaatgccgta agttaacagg agtgtgccgc
     9901 actacacgct acgaactgga gaaaaaaggg aatttcccgt cacgccgtaa tctgggtggt
     9961 cgttcagtag gttggctgct ttccgaggtt atggaatggg ttaaaagccg ggatcgtatt
    10021 aattcaggta aggcagcgta gggggaacat atgacacata aaacaaaggc gacctgtggc
    10081 ggtcgccaat gggaaaacac taaacataag cccgaacatc atagcgattt gctgtctggt
    10141 gggcaatgtg ctcagtgtgc tggcttgctg gtggactatt cctgctcttt ggccttgcgg
    10201 cgctggcggc gtttgatctc gccttcaagt gccgaaacga taaattgccc agtgctttcg
    10261 ccttcttcct ttaaattttc tacggcgtca gctattgcat gggggactcg tgcctcaagt
    10321 tttttggatt ttccattaac tgctttcgtt gccatatctg atttccttgt tattaggtgg
    10381 ctgacagtat acacacaaga aaatggaaaa acactattga cgtggctgac acctgttact
    10441 agtatagtgg ctgacacctt tattgaggta atcgatgaaa agacaaagcc cgcaggtgct
    10501 accaacacca acgggcttct aacaaccaac gatagcaaca gtatcgaggt agctatgaga
    10561 aatcatacca cacacccgca agggcgggac tcgcacaacc tgaataaata catctggcgt
    10621 tttctggccc tgagtgccgg agaatctcaa atcatccacg taaccgcctg gacggaacgc
    10681 gaagcgcgta gccgttgccc gtcaggttgt gttgctgtat ttgtcgcccg tattcgtcag
    10741 ggggtgtgcc gtgcttaaaa ccttccgcgt tttttcccgt gctgttaaca agcgcggcta
    10801 taccgttggc atcacgcaga acgtgaaggc cgttaatatg caagcggcga ttgattcagt
    10861 aatcagtaat tccggtaaag caggtttatc acatgtaatt attgcagcag tacatgaatt
    10921 aaaagggggg gcattaatga aggaaattac attacacgaa gccgctgaac gtgcgcacca
    10981 gacagaaatt atttgccgac ttcttgaggt gtaccctaat aaattaaatg atggtgatat
    11041 atccgcatta gctggccttc tggcaaaact ttctggaagt gttgcgctgt ggttaattga
    11101 tgagagattt aaacgctatg aacaataaca aacgcgtaat ttgcgaattg aatgaattca
    11161 cacaaagcgc aatatcttac aacaatgccg taagtgagat tattaacgtc tggtttgaat
    11221 cgctgacaaa ttgcgagcgt gacgagtcaa cgacaagatt aacctttgcc atttctgaat
    11281 tactgaatat ttccaccagc aaaatagaga gcatatcaga gaagattaac ggcttatcag
    11341 gtcacgctat cggagaataa aaataatgaa acttaaatat tctggcttaa ctgccagcgg
    11401 caaaactcgc actaaattca tgtgcgggga tatttaccgc gataagtacg gcggcacggt
    11461 aatgattaag ggcgtggagg aacggcgcgt aacttaccgc cgtgaaggct acgaatatga
    11521 ttgcgttatg cctgtttatc agttcaaccg tgatttttct ctggtacagg cagcgccccg
    11581 cagtaagcca accagcaggg agaaagcacg cgccaatatt caggaaataa aaaagatgct
    11641 taacgtattc aggggtaaaa aatgaaactg gcaccgaacg taaaaaaaca gccacgcgga
    11701 ataaaacaca aagacacaga ggtgattatt ttcgcgggta gtgatgcgtg ggcacatgca
    11761 aaacagtggc aggagcagga cggccccgca tccggcgata atgtgcctcc tgtgtggctt
    11821 gggccaaatc agcttgccga actttatgca ctgcaaattg ttcctgatgg aaaaaaacgc
    11881 gtaaggctgt accaggcagg agaactggac ttggtggaga ccaaaaagat tggtcagaag
    11941 ctggcggcgg caggcgtaca ggatgcaaaa ttttaccccg aaggaatgca cggtcagaag
    12001 tgtgaaaact ggcggcgcta tctgaatgct gagcgtgaaa atattgccgc agggcttacc
    12061 atgccggagc agaaaaatac gcaactggca caaatggcag acagtgagcg cgcccagctg
    12121 cttgccgatc gctttgatgg cgtttgcgta catcctgaaa gtgaaatcgt tcacgtatgg
    12181 cgcggcgggg tatggtgtcc ggtcagcaca atggaactta gccgcgaaat ggtggcgatc
    12241 tattcagagc acagggccac tttcagcaaa cgcgtgatca ataacgccgt tgaagcgtta
    12301 aaagttattg ccgaaccaat gggggagccg tccggcgatt tgctgccgtt cgccaatggt
    12361 gcacttgacc tgaaaacggg ggaattttcc ccgcacacgc cggagaactg gatcaccacg
    12421 cacaacggca ttgagtacac gccaccagca ccaggggaga acattcgcga caacgcgcca
    12481 aactttcata aatggcttga gcacgcagcc ggaaaagacc cgcgcaagat gatgcgtata
    12541 tgtgccgcgc tgtacatgat tatggcgaac cggtacgact ggcagatgtt tattgaggcc
    12601 accggagacg gcgggagcgg taaaagtaca ttcacacaca tagccagcct tctggcaggg
    12661 aaacagaaca ccgtaagcgc ggagatgaca tcactcgatg atgcaggagg gcgcgcgcag
    12721 gttgtcggga gtcgtcttat cgtcctggct gaccagccga aatatacagg cgaaggaacg
    12781 ggcatcaaga aaatcacggg cggcgacccc gtggaaatta acccgaaata tgaaaagcgt
    12841 ttcacggcgg taatcagggc ggtggtgctg gcgaccaaca acaacccgat gatattcacc
    12901 gaacgggccg gaggcgtggc acgtcgtcgg gttatttttc gctttgacaa tatcgttagc
    12961 gaggcagaaa aggacaagga tttgccggag aaggtcgcgg ctgaaatccc tgtgattatc
    13021 cgccgcttgc tggctaattt tgctgaccct gaaaaggcac gggctttatt actggaacag
    13081 cgcgacggtg atgaagcact ggcgataaag caacagacag atccggttat tgagttttgc
    13141 cagttcctga attttctgga ggaagcgcgc ggcctgatga tgggtggcgg tggcgattcc
    13201 gtgaagtaca cgaccagaaa cagcctttac cgcgtctatc tggcgtttat ggcatacgca
    13261 ggaagaagca agccgctaaa cgtggctgat ttcagcaagg ccatgaagcc agcggcgaaa
    13321 gtttacggac atgaatatat tacgcgaaaa gttaagggag taacgcagac caacgcaatt
    13381 acaacggatg attgtgacgc gtttttataa ttttttgtaa aagccctcta ccccatctac
    13441 ctgaatgaaa taaacgcata ttattcaaca tgataagtgg gtagagggat aggtagaagg
    13501 ctaataaaac ccctctaccc cctctaccat gattaaggtc gtttgtgagg ggcgggtaga
    13561 ggatgggtag agggccacga aaagtcctct acccatctga aagccgcgcc attactgacc
    13621 tgaaagatga ttaggtagaa gaggtagagg aggtgcacca caacctaaaa ctttttaaac
    13681 gagggggggt aaaaataaat gtgcatacat caaaatcact taacaaacat gccgaccgaa
    13741 aacatgaatc aggggcgaca aatgaccaaa atgcgcagag acagaacaca gcaaaaatat
    13801 aaagcgttag acatgacaga gctttcccta aaggtggcaa tcaaagttat agaccgccac
    13861 acacgggaag gatacgcgaa ggagaatccc gaactaataa gcgcattcat gaccacggcg
    13921 gcggcaaact ttgccacgct gacagagcgg gagattgcag aagcggaaca ggtaacaacc
    13981 atcaacgtta aaagcactgg agaataaccg aaggagtgag cacatagccg gagcaatccg
    14041 gctttttttc gatctctcat atcttaaaaa atggttgatt aatgataatc tatcgaaaat
    14101 actgattatg cgatgataag tatcaggagg aaacaaagtg gaagagataa agcaaagcgc
    14161 taccgtgatg attgatcacg agatcctaaa cgagcttaaa aaacttcaat tcgatgttat
    14221 tcgccggatg aatgaagctg gtatatacgg cagagccgcc acgccaacaa taggtcatct
    14281 tgcacgtgtg ctgttacgcg aaagactggg cattgcaaca ggtgaggcat aacaaatggc
    14341 tatcacagaa cgcgaggcaa gcatcattaa ggccattggc gaggaaacaa gagacaccat
    14401 agcgccattg atggaaaaga tcgcagagct tgagacacga ataaacaagc tgagtaatga
    14461 agttgaaggc ctgatctcgt tactcggtga gcaatgacat ggggggggag tgttccggcc
    14521 ggaaagtcga tcaccctgga caccgccccc cgtctcattc gtagaaaatt ttgcttttca
    14581 aaaaagttac acgaaaagtt acaccccctc aaaacgaggt ttttgcatgg cattaacgac
    14641 caaaaaaaag cggtttgctg atgcgttatc aaggggcgca aatcccacgg aagccgctat
    14701 acatgcggga tacagcgaaa agaccgcaag ggttaaaggc tggcagctat ccaacgatcc
    14761 tgaggtgaaa aagtatctta aggctgtaac ttctgatgta acttttcagg ttacaaaacc
    14821 gaaaaaagtt acaaagaaag ttaccgaaaa agttacaccg gaacgggcat caattaacac
    14881 cattgagatg atggataacg gcctacccga ccccatcaaa gcaatgggca ggatactgat
    14941 tgacaacatg gacacagatc caaaactggc tttagatgcc gcgtttaagc tggcccagtt
    15001 cacccaccgc aagatcggca gcattgggaa aaaagaggtt aaaaaactca atgccgctag
    15061 tgtctactca aatcggttcc ccatccctaa gccaccaaca gacttaaaac actgaggtag
    15121 cgaactatga gcgaatacat cacagaccct attgatgtac tcacggaaat catgaacgac
    15181 aagcagcagt cgaccgaaat acggttacag gccgccgctg ttctgatgcc atattttcac
    15241 gaaacactaa acccgtgctt ccaggaggag gaagaagatg atctgtaaaa acatgaaagg
    15301 cgtgtcagca gttcgcgccg ccagcgtgct ggcatcgtgt aaatttagcc atgctgacgc
    15361 tatagagatg acgaaaagaa atgatccggc agtatcgaaa tttcttgaaa aatccatcgt
    15421 caacagaatg actacggata acatgccgtc gctggtggca gagccgatag taagagagct
    15481 tggcgagtat attttttcgc agtctgtacc aggcaagcta atcagcagcg cgcttaatct
    15541 tccgtttggc gcaaaattgc attcaatatc aggcaagggt agtaaatggc ttaaagaagc
    15601 cgaagaaatc cctgtcaaag agggagaagt aaacgaaagt gcgctaaaat tgtgcaaact
    15661 tggcgctatt gtgatgctga ataacgagct aatcaaattt agcacccctg gtacaaattc
    15721 agcgatcaga gacttgatca catcggaatc ggtgaaagaa atagataaaa aatttctttc
    15781 cagtgatcag gaggtggctg gcgtatcccc tgagggcgtg cttaatggtg ctgatgaggc
    15841 cgaaaacttc atagagattt tcaaaaagca catagcgaac ggtaacacgc tttccacgtc
    15901 gtcgctaata ttgcccgtag aaaacgcctt acagcttact gatgctgagt tctggcagat
    15961 agagcttttg agaataccgg taattgtgag cgaacaggcg gatcgcatga tgctgataga
    16021 cgctcaaaag ctgattatta acgttgaagc cacggttata accccaactg atgaggcggt
    16081 aataaaaacc gaatcagggg cggttagcct tttccagaat gacgctacag cgttcagggc
    16141 aatcacctat tgcggatggc aaaaactcga taaggccgtc accgttctgg cgaaaagctg
    16201 atttttccag catctatccc gatatgggga tcaccatatc ggggaagcac ctgaatcatc
    16261 attaccgtga tcaagggtat gtaaagccca ccagcctgat taacgtttaa ccggaaaaaa
    16321 agccaggtat ccaatctcga tatggggatc accatatcgg gatacggcgg taactggttg
    16381 atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgattctt
    16441 cccaatttcc acagactgcg gaaattggcg cggtaactta ctgagattaa agcaaagcgc
    16501 aaaattgcgt tggctggtgg gcctgatgcc gataactggt tggaattgtt ccatagtgca
    16561 aaattgcact atggcctaac ccattgatta tttcgaaaac ccccattggg ggatgttggg
    16621 aaagtaagac taacccattg attcttccac aatcctcaat ttgaggagat cggaaacatc
    16681 acccttcaaa ttctgaatgg tcataatgac cacgcagacc atcaaggcaa ccacaacgaa
    16741 catcgttacg gttggagctt ttaccccact ggggaaaagg tacgcacctg aatcatcatt
    16801 accgtgatca agggtatgta aaggccacca gcctgattaa catttaaccg gaaaaaaagc
    16861 caggtaggtg gggataccca tatcggggaa catccaccag cttcataacg gatggccgga
    16921 aaatgataac ggttaaagcg tgttgatctt tgttcgtggt tgttcgatat tgttcgtgaa
    16981 acggtgtagt taatggtgta gttaatttcg ctgtttttga cggagttgtt atttgtaaca
    17041 tagtgaaaaa taaagactaa tcaatgaaat gttataaatg atagttatct atcatgtgga
    17101 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
    17161 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
    17221 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
    17281 tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
    17341 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
    17401 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
    17461 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
    17521 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
    17581 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
    17641 cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga
    17701 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
    17761 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccaatc
    17821 ctccggtcgc aatgaggtaa ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
    17881 gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt
    17941 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
    18001 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
    18061 tcagcttgcg cgcgtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg
    18121 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg
    18181 tgtcggtaaa accgcgattg cggaaggtct tgcctggcga attgttcagg gcgatgtgcc
    18241 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
    18301 taaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga
    18361 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gggcagcgtc
    18421 tggtggccag gtcgatgcgg ctaacctgat caaaccgttg ctctccagcg gtaaaattcg
    18481 cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
    18541 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat
    18601 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
    18661 ggtgcgtgca gcggtacagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
    18721 agccattgac gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg
    18781 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cgcgcattcc
    18841 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
    18901 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
    18961 gcgtgcaggt ttaggtcacg aacataaacc ggttggttcg ttcctgtttg ccggccctac
    19021 cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagctgct
    19081 gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
    19141 tccgggatac gttggttttg atcagggagg tttgctgact gatgcggtca tcaagcatcc
    19201 acatgcggtg ctgttgctgg acgaaatcga gaaagcgcat ccggacgtgt tcaatattct
    19261 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
    19321 taacgtggtg ctggtgatga ccaccaacgc cggggtacgt gaaactgagc gtaaatccat
    19381 tggtcttatc caccaggata acagcaccga tgcgatggag gagatcaaga agatctttac
    19441 accggaattc cgtaaccgtc tcgacaacat tatctggttt gatcatctgt caaccgacgt
    19501 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
    19561 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
    19621 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
    19681 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa
    19741 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
    19801 gcattaatct gattgttagg taggttggtc aagtccgtaa tctcgaaaga ggttacggac
    19861 tttttgttta tggggtggag gaggttcaga cccttttttt aatgatgatg gtaagttgtt
    19921 gataattagt gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt
    19981 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgcca tacaggcagc
    20041 ttagaaatcg acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga
    20101 taaaatgcga aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcggtgag
    20161 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
    20221 ccactcggac acctcaccaa attgttttgt cgcctgacct catgggtggc aacggggcgc
    20281 tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct
    20341 catactgtaa acaagttgtg cagtatatct acatcgagac aagttacgga cttatacttc
    20401 caaagtactt catacatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt
    20461 ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt
    20521 tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt
    20581 agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta
    20641 cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt
    20701 ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc
    20761 gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat
    20821 tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga
    20881 taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta
    20941 accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc
    21001 ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat
    21061 ttgattgaga taattaaggt aatcccggcg gggaatttcg caggcaccaa gcgatgctgt
    21121 gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca
    21181 gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgctaca
    21241 aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac
    21301 ttcaatggag tgggcatggc cgagttcgtg aaggcgatgg taggcttcga ccacgccacg
    21361 cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc
    21421 gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat
    21481 atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc
    21541 gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct
    21601 aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg
    21661 gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag
    21721 tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg
    21781 gcaagcagct ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt
    21841 tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc
    21901 atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta
    21961 ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg
    22021 cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca
    22081 tcctcgagca gcttctccag gccaacgcga cgcaagctct ccgccagagc ctcatcacta
    22141 ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc
    22201 tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta
    22261 tcgttaagca aaatctcgcc ctgttgcggg tcccaagcgc gggtcagcag ttgtaacagt
    22321 gttgatttgc cgcatccggt tcgcccgaga atcgcaatat gttccccggc gtttacctga
    22381 agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt
    22441 aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt gacttccggt
    22501 ttttgatccg ttaaatcagt gatccgaacg gcagaggcaa tgacttgccc cagatgctga
    22561 aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcagaa gacaaacagg
    22621 gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc
    22681 aggatcaccg ctaacgcgcc aatgagtagc attatcgctt gcgacaatgc ggtcagttca
    22741 gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa
    22801 cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt
    22861 tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa
    22921 aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg
    22981 gtgaaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc
    23041 agcggcgaga taacgcgcag ataaagatga tcgagcgtat caacatccgc caccacgcga
    23101 ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg cagcaatttg
    23161 ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga
    23221 cgttcaaaat aacgcccggc agtacgggtg attgctgcgc cacgcacgcc cgcagcgggt
    23281 agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac
    23341 cagccggaaa gtgtcaacag accgatactg gcgagcagcg tgacaattgc cagcacgata
    23401 ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc
    23461 atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt
    23521 aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca aatgacatcc
    23581 cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg
    23641 gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca
    23701 tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc
    23761 tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg
    23821 agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg
    23881 gcatcaggtc gcgccagtag tacgttatcc cgcaatgttg ctgccggtaa ttgtgggttt
    23941 tgcccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg taattctatc
    24001 ccgttgattc gtaatgatcc ctgatatgaa agaaaaccag aaagcgcgtt cagcagtgag
    24061 cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg caaagtaaag
    24121 ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca
    24181 atggtcaccg gatcggtcga tgctaattcc gcctcaccgc gctgcggatg ggcgagcggg
    24241 gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa
    24301 aacgtaccga gatcgcgcaa tggctggaaa aactctggcg caaggatcag ggccagaaaa
    24361 cccgcagcca gcgtcacacc ggtatcgtag tgaccaaaat ccagctcgcc gagataggaa
    24421 aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg
    24481 gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca
    24541 gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg
    24601 cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgccggtta
    24661 gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg tgctgtgccc
    24721 agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc
    24781 accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg catatcgtca
    24841 atttgctcga gtaccaacgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc
    24901 cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg
    24961 gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac aaaggtcaga
    25021 accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat aatcatatgt
    25081 tgcagaattc gcgccatgaa ccaggcctgg gcaataatca atatgccgct tacaaagccc
    25141 agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg
    25201 gttaactctt tttgacggga tttattcatt gcacgcttag caggtgagtt atcagaatta
    25261 tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg catggtgtcg
    25321 ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt
    25381 agcgttccgc atcaagtgct gccatgcagc ctgtaccggc cgaagtaatg gcctgacgat
    25441 aaatatgatc catcacgtca cccgcggcaa agacgccagg gatgctggtc tgggtggcat
    25501 taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc tgcccttcga
    25561 aaatggcagt attcgggctg tgaccgatag caacaaacag accagcaacg tcgagtgact
    25621 cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc atttgatcgc
    25681 cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc tccactttat
    25741 ccatcagacg cttaataagg attttttccg cgcggaaacc gtcacggcgg tgaatcagat
    25801 gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc
    25861 cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca caagcagaaa
    25921 ccccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca gaagctccgg
    25981 tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga cggaacggac
    26041 ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca aacttggtgg
    26101 catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga tcgccaggcc
    26161 agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca
    26221 caggttgcag gttggcgcgc gccgcgtaga cagcagcggt gtatcccgcc gggcctgaac
    26281 ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag
    26341 acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac gattttgtta
    26401 acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc
    26461 tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat
    26521 gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt
    26581 gcgaaaacat tcgaggaaga aaaaaaacag tattcttata tgcgcataac catgcatgta
    26641 aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg ccattcgtga
    26701 tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat tgcgcgccgt
    26761 gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg
    26821 gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat agcaagaagc
    26881 gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg caaaaggatg
    26941 ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg ccgtgccttg
    27001 agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg ctgcttaacc
    27061 cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat cgtggcgcac
    27121 cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt caggagtgtc
    27181 atttagtatc tggtgatttc gactacctgt tgaaaacacg cgtgccggat atgtcagctt
    27241 accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac acacggacat
    27301 acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg cgctaacacg
    27361 gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac agcaacagta
    27421 ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg
    27481 agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga caaagttaag
    27541 tagcggccgt cgccttctgg aagcgttgct gatccttatt gtcctgtttg ccgtctggtt
    27601 gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa cggcctggca
    27661 tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ctggcagata cgctgttctt
    27721 tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt gttggtttgc
    27781 ctggcgtcat cagtccagtg acgaatacat tgattatttt gccgtttcgc tacgcatcat
    27841 tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg ctgacgatat
    27901 ctggtatttt gcctccggtg gcgtcattgg cagcttacta agcactacgc tacaaccact
    27961 gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggctgcgg gcctgacgct
    28021 gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga ttttaaacat
    28081 tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg aagatgaata
    28141 tgaagatgac gaagagtatg aagatgaaaa ccacggcaaa cagcatgaat cgcgccgtgc
    28201 ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc
    28261 gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg atgacgaaga
    28321 gattatctac actgcacgcg gtgtggctgc cgacccggac gacgtcctat tttcgggcaa
    28381 tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg cgccaattac
    28441 cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg ctgcgccggt
    28501 tgaacctgtg actcagacgc cgcctgttgc ctctgttgat gttccacctt cgcaacctac
    28561 agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg ctcctgcacc
    28621 ggaaggttac ccacagcagt cacaatatgc gcagcctgca gtgcaatata atgagccgct
    28681 gcaacaacca gtacagccgc agcagccgta ttatgcacct gcagctgaac aacctgcgca
    28741 acaaccttac tacgctcctg cggcggaaca acctgttcag caaccgtatt acgcccctgc
    28801 gccagaacaa ccggtggcag gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc
    28861 tccacagtct acataccaga ctgagcaaac ttatcagcaa ccagccgctc aggagccgtt
    28921 gtaccaacag ccgcaatccg ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga
    28981 agagacaaaa cccgcgcgtc cgccgcttta ctattttgaa gaagtggaag agaagcgagc
    29041 ccgtgaacgt gaacaacttg cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc
    29101 agaaccgatc aaatcttcgt tgaaagcgcc ttctgttgca gcagtacctc cagtagaagc
    29161 cgctgccgct gtttctccgc tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc
    29221 tgccgcaacc gttgccgcgc cagtcttcag tctggcaaat agcggtggac cgcgtccgca
    29281 agtcaaagag gggattggcc cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg
    29341 tcgtgaactg gcgtcttacg gtattaagct gccctcacag cgtgcggcgg aagagaaagc
    29401 ccgtgaagcc cagcgcaatc agtatgattc tggcgatcag tataacgatg atgaaatcga
    29461 tgcgatgcag caggatgaac tggcacgtca gttcgcccag acacagcagc aacgctatgg
    29521 cgaacagtat caacatgatg tgcccgtaaa cgcagaagat gcagatgctg cggcagaggc
    29581 tgaactggct cgtcagtttg cgcaaactca acaacaacgt tattccggcg aacaaccggc
    29641 tggggcgaat ccgttctcgc tggatgattt tgaattttcg ccaatgaaag cgttgctgga
    29701 tgatggtcca cacgaaccgt tgtttacgcc aattgttgaa cctgtacagc agccgcaaca
    29761 accggttgca ccgcagcagc agtatcagca gccgcaacaa ccagttccgc cacagcagca
    29821 atatcagcag ccgcaacagc cggttgcgcc gcagccgcag tatcagcagc cacaacagca
    29881 ggttgcgccg cagccgcagt atcagcagcc acaacaaccg gttgcgccac agccgcaata
    29941 tcagcagccg caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt
    30001 tgcaccgcag cagcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg
    30061 tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag
    30121 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg
    30181 tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac
    30241 tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg
    30301 ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
    30361 accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt
    30421 tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga
    30481 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg
    30541 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
    30601 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
    30661 ttatgaaggc attccgcatc tgttaacaga agtcgttact gatatgaaag atgccgccaa
    30721 cgcgctgcgc tggtgtgtta acgagatgga gcgtcggtat aaactgatgt ctgcgctggg
    30781 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
    30841 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
    30901 aaaagaacca tacattgtgg tgttggttga cgaatttgcc gacctgatga tgacggtagg
    30961 taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca
    31021 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
    31081 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
    31141 tcaggctggc gcggaatcac tgctggggat gggggatatg ctctactctg ggccgaactc
    31201 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
    31261 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt ccgacagtga
    31321 aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
    31381 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt
    31441 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
    31501 cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt
    31561 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
    31621 agtattttct tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc
    31681 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat
    31741 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
    31801 gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
    31861 gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
    31921 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
    31981 ataacccgtt cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc
    32041 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
    32101 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
    32161 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
    32221 gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
    32281 tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
    32341 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
    32401 gaaaatttag cacagtatat cggccagcaa catttgctgg ctgcggggaa gccgttgccg
    32461 cgcgctatcg aagccgggca tttacattct atgatcctct gggggccacc gggtaccggc
    32521 aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
    32581 gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
    32641 aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag
    32701 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
    32761 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg
    32821 aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacg
    32881 cgtggctatg gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa
    32941 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
    33001 gaagtcaatg atagcggtaa gcgggtcctg aagcctgaat tattgaccga aatcgccggt
    33061 gagcgaagcg cccgctttga taacaaaggc gatcgctttt acgatctgat ttccgcactg
    33121 cataagtcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt
    33181 accgccggtg gcgatccgtt atatgtcgcg cgtcgctgtc tggcgatagc gtctgaagat
    33241 gtgggtaatg ctgatccacg agcgatgcag gtggcaattg cggcctggga ttgctttact
    33301 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
    33361 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagccg cgctggcgga tgctcgcgaa
    33421 cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
    33481 atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt
    33541 gaggtttact tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc
    33601 cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc
    33661 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
    33721 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
    33781 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
    33841 ctggatgtag ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg
    33901 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
    33961 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
    34021 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct aaccatccct
    34081 aacttgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc
    34141 cgctggggca ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa
    34201 atgcactctg gcctcgactt tgcagccgca gttaagctga caggttcgcg ctttgtggtg
    34261 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
    34321 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
    34381 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa
    34441 gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgaccaac
    34501 ctggtacgcg gtgaaatcat tgatgaagat gatctgccaa ttaagatgac cgcccacacc
    34561 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
    34621 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg
    34681 ctggaagaga tgactggtca tgcagaaaaa gtcctgcaac tgctgggcct accgtaccgt
    34741 aaaatcatcc tttgtaccgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa
    34801 gtatggatcc cggcacagaa cacctaccgc gagatctctt cctgctccaa cgtttgggat
    34861 ttccaggcgc gtcgtatgca ggctcgttgc cgcagcaagt cggacaagaa aacccgtctg
    34921 gttcataccc tgaacggttc tggtctggct gttggtcgta cgctggttgc agtaatggaa
    34981 aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
    35041 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagc
//
LOCUS       DAZGCS010000048        34230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGCS010000048 DAZGCS010000000
VERSION     DAZGCS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.048"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..93
                     /gene="tnaC"
                     /locus_tag="ACHK01_002397"
     CDS             19..93
                     /gene="tnaC"
                     /locus_tag="ACHK01_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO8097341.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            314..1729
                     /gene="tnaA"
                     /locus_tag="ACHK01_002398"
     CDS             314..1729
                     /gene="tnaA"
                     /locus_tag="ACHK01_002398"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO8097342.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHK01_002399"
     CDS             1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHK01_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO8097343.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHK01_002400"
     CDS             3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHK01_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO8097344.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHK01_002401"
     CDS             4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHK01_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO8097345.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5465..6214
                     /gene="yieE"
                     /locus_tag="ACHK01_002402"
     CDS             5465..6214
                     /gene="yieE"
                     /locus_tag="ACHK01_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO8097346.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6236..6802
                     /gene="yieF"
                     /locus_tag="ACHK01_002403"
                     /gene_synonym="chrR"
     CDS             6236..6802
                     /gene="yieF"
                     /locus_tag="ACHK01_002403"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO8097347.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHK01_002404"
     CDS             complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHK01_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO8097348.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8360..9025
                     /gene="yieH"
                     /locus_tag="ACHK01_002405"
     CDS             8360..9025
                     /gene="yieH"
                     /locus_tag="ACHK01_002405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO8097349.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9092..9565
                     /locus_tag="ACHK01_002406"
     CDS             9092..9565
                     /locus_tag="ACHK01_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097350.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9614..10201
                     /gene="cbrC"
                     /locus_tag="ACHK01_002407"
     CDS             9614..10201
                     /gene="cbrC"
                     /locus_tag="ACHK01_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO8097351.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10263..10985)
                     /gene="yieK"
                     /locus_tag="ACHK01_002408"
     CDS             complement(10263..10985)
                     /gene="yieK"
                     /locus_tag="ACHK01_002408"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8097352.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11000..12169)
                     /gene="yieL"
                     /locus_tag="ACHK01_002409"
     CDS             complement(11000..12169)
                     /gene="yieL"
                     /locus_tag="ACHK01_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8097353.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12196..13812)
                     /gene="bglH"
                     /locus_tag="ACHK01_002410"
     CDS             complement(12196..13812)
                     /gene="bglH"
                     /locus_tag="ACHK01_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO8097354.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNAIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13898..15292)
                     /gene="bglB"
                     /locus_tag="ACHK01_002411"
     CDS             complement(13898..15292)
                     /gene="bglB"
                     /locus_tag="ACHK01_002411"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO8097355.1"
                     /translation="MKAFPETFLWGGATTANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVSKTRGLSLKK"
     gene            complement(15311..17188)
                     /gene="bglF"
                     /locus_tag="ACHK01_002412"
     CDS             complement(15311..17188)
                     /gene="bglF"
                     /locus_tag="ACHK01_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO8097356.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17321..18157)
                     /gene="bglG"
                     /locus_tag="ACHK01_002413"
     CDS             complement(17321..18157)
                     /gene="bglG"
                     /locus_tag="ACHK01_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO8097357.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18443..19168)
                     /gene="phoU"
                     /locus_tag="ACHK01_002414"
     CDS             complement(18443..19168)
                     /gene="phoU"
                     /locus_tag="ACHK01_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO8097358.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19183..19956)
                     /gene="pstB"
                     /locus_tag="ACHK01_002415"
     CDS             complement(19183..19956)
                     /gene="pstB"
                     /locus_tag="ACHK01_002415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO8097359.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20047..20937)
                     /gene="pstA"
                     /locus_tag="ACHK01_002416"
     CDS             complement(20047..20937)
                     /gene="pstA"
                     /locus_tag="ACHK01_002416"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO8097360.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20937..21896)
                     /gene="pstC"
                     /locus_tag="ACHK01_002417"
     CDS             complement(20937..21896)
                     /gene="pstC"
                     /locus_tag="ACHK01_002417"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO8097361.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21982..23022)
                     /gene="pstS"
                     /locus_tag="ACHK01_002418"
     CDS             complement(21982..23022)
                     /gene="pstS"
                     /locus_tag="ACHK01_002418"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO8097362.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23336..25165)
                     /gene="glmS"
                     /locus_tag="ACHK01_002419"
     CDS             complement(23336..25165)
                     /gene="glmS"
                     /locus_tag="ACHK01_002419"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO8097363.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(25327..26697)
                     /gene="glmU"
                     /locus_tag="ACHK01_002420"
     CDS             complement(25327..26697)
                     /gene="glmU"
                     /locus_tag="ACHK01_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO8097364.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(27048..27467)
                     /gene="atpC"
                     /locus_tag="ACHK01_002421"
     CDS             complement(27048..27467)
                     /gene="atpC"
                     /locus_tag="ACHK01_002421"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO8097365.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(27488..28870)
                     /gene="atpD"
                     /locus_tag="ACHK01_002422"
     CDS             complement(27488..28870)
                     /gene="atpD"
                     /locus_tag="ACHK01_002422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO8097366.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(28897..29760)
                     /gene="atpG"
                     /locus_tag="ACHK01_002423"
     CDS             complement(28897..29760)
                     /gene="atpG"
                     /locus_tag="ACHK01_002423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO8097367.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(29811..31352)
                     /gene="atpA"
                     /locus_tag="ACHK01_002424"
     CDS             complement(29811..31352)
                     /gene="atpA"
                     /locus_tag="ACHK01_002424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO8097368.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(31365..31898)
                     /gene="atpH"
                     /locus_tag="ACHK01_002425"
     CDS             complement(31365..31898)
                     /gene="atpH"
                     /locus_tag="ACHK01_002425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO8097369.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(31913..32383)
                     /gene="atpF"
                     /locus_tag="ACHK01_002426"
     CDS             complement(31913..32383)
                     /gene="atpF"
                     /locus_tag="ACHK01_002426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO8097370.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(32445..32684)
                     /gene="atpE"
                     /locus_tag="ACHK01_002427"
     CDS             complement(32445..32684)
                     /gene="atpE"
                     /locus_tag="ACHK01_002427"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO8097371.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(32731..33546)
                     /gene="atpB"
                     /locus_tag="ACHK01_002428"
     CDS             complement(32731..33546)
                     /gene="atpB"
                     /locus_tag="ACHK01_002428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO8097372.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(33555..33935)
                     /gene="atpI"
                     /locus_tag="ACHK01_002429"
     CDS             complement(33555..33935)
                     /gene="atpI"
                     /locus_tag="ACHK01_002429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO8097373.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         8459 a         8890 c         8283 g         8598 t
ORIGIN      
        1 ttcgaggata agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat
       61 atcgacaata agattgtcga tcaccgccct taatttaccc ttctgtagcc atcaccagag
      121 ccaaaccgat tagattcaat gtgaacgatt agtttgctat atcttaattt tgccttttgc
      181 aaaggccatc tctcgtttat ttacttattt tagtaaatga tggtgcttgt atatatacat
      241 ggcgaattaa tggatattgc agatgtaata ttcacaggga tcactgtaat taaaataaat
      301 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      361 tgagccagta aaacgtacta ctcgcgctta tcgtgaagaa gcaattatta aatccggtat
      421 gaacccgttc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      481 cggggcggta acccaaagta tgcaggcagc gatgatgcgc ggcgacgaag cctacagcgg
      541 cagccgcagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      601 cattccgact caccagggcc gtggcgcaga gcaaatttat attccggtac tgattaaaaa
      661 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcattctcta actatttctt
      721 tgataccacg cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa
      781 agaagccttc gatactggcg tgcgttacga ctttaaaggt aactttgacc ttgagggatt
      841 agaacgcggt attgaagaag ttggcccgaa taacgtgccg tatatcgttg caaccatcac
      901 cagtaactct gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      961 cgcgaagaaa tacgatattc cggtggtaat ggactccgca cgctttgctg aaaacgccta
     1021 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1081 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtaccgat
     1141 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
     1201 cctttgcgtg gtgcaggaag ggttcccgac atatggcggc ctggaaggcg gcgcgatgga
     1261 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
     1321 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1381 cggtcacgcg gcattcgttg atgccggtaa actgctgccg catatcccgg cagaccagtt
     1441 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
     1501 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
     1561 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1621 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
     1681 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
     1741 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
     1801 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
     1861 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
     1921 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
     1981 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
     2041 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     2101 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     2161 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     2221 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     2281 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     2341 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     2401 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     2461 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     2521 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     2581 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     2641 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     2701 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     2761 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     2821 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     2881 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     2941 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     3001 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     3061 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     3121 atgtccaaat ggcaactcgc ctgattctcc ttcaccacgt atgctttgcg tcaccttact
     3181 attaggacgc tttagcccat gtcccgcttt ttaatttgta gttttgccct ggttttactt
     3241 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccga cgatctcaat
     3301 gccagcgaag cgcagttgca tattgcgttc tccgtttatc tggcgggcat ggcagctgcg
     3361 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agcctgtcgc catccccggc
     3421 gcggcgctat ttattatcgc ttcggtgttc tgttcactgg ctgaaaccag cacgttattt
     3481 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     3541 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     3601 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     3661 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     3721 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     3781 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     3841 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     3901 tttgagcgcg gagaatacgc caccattatg gcgttgactg ctggcgtcag catgaccgtt
     3961 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     4021 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     4081 ctgtttggta tcacgctgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     4141 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     4201 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     4261 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     4321 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     4381 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     4441 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     4501 cgacccgctc tttgtgaact caccgctggg cctgtcgccc acaccgctaa tggtcagcat
     4561 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     4621 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     4681 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     4741 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     4801 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     4861 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     4921 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     4981 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     5041 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gcagcgcaac ccgacaatct
     5101 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     5161 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     5221 cctgctgtgg cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat
     5281 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     5341 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     5401 gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg
     5461 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     5521 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     5581 tctggcgtct agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     5641 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     5701 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     5761 tggtctcgat atggaactac agcgtgcgac gcgcgggttt cacagcccac acgcgcccga
     5821 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     5881 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     5941 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     6001 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     6061 cacgcccacg attgaaaagc tcagtgtctg ggagttcgat ggcaaacacg gctggaaaag
     6121 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     6181 gctctctacc gtgaaggctt tttcgccaaa ttgatagaca accacaggag tcatcatgtc
     6241 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     6301 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     6361 cattgccgac attcccttgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     6421 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     6481 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     6541 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     6601 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     6661 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     6721 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     6781 tattcagcga gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt
     6841 aacgcgctat cgattttagt gagcgtcgat aaagacaatc ttcaggataa acagcagcgc
     6901 aacgatgatt acgcacgggc ttagatcacg cagacgtccg gtaccgattt tcatcacgca
     6961 gtaggagata aagcccagcg cgataccttc ggtaatcgag aagctgaacg gcatcatcac
     7021 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gtgccagact
     7081 ggaagtcatc agcacgccaa cgtaaatcag cgcaccagct gcagcgtagc ctggcaccat
     7141 ccccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     7201 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     7261 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     7321 catgcgcggg aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag
     7381 cgtaccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     7441 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     7501 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     7561 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     7621 gccgaggatc cccagcagca cgctgtgaga agtcaggtta ccgatgctga ccagcgtttc
     7681 cgggttagcg acaatcacac ctgcattctt cagtcccatc ataccaatga acagaccgat
     7741 accgctggtg atgcccacac gcagacttac cggaatgttg gcaatcatcc agtagcgaac
     7801 gcggaaaatc gtcagtaaca gcagaccaat cgcaccccag aagattgcgc ccatcccgac
     7861 ttgccacggc aagcccatcg cctgtacaac gacaaaagcg aagaacgcat tcaggcccat
     7921 agcgggtgcc agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc
     7981 agcgatcaga caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     8041 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     8101 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     8161 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     8221 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca
     8281 ttgtatttgc ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa
     8341 ctaacgataa aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac
     8401 gctggtcgac agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg
     8461 tattacgctc gatcctgaag aggtattcaa acgtttcaaa ggtgtaaaac tgtacgaaat
     8521 tatcgatatt gtttcccttg aacatggtgt tacgttagcg aaaacagaag ctgaacacgt
     8581 ttaccgtgca gaagtcgctc ggctgttcga ttcagaactg gaagccatcg aaggggctgg
     8641 agcgctcctg tcagcgatca ctgcgccaat gtgtgtggta tctaacggcc caaataacaa
     8701 aatgcagcat tctatgggca agctgaatat gttgcactac ttcccggata aactgttcag
     8761 cggctacgat attcagcgct ggaagccaga cccggcgtta atgttccatg cggcaaaagc
     8821 gatgaatgta aatgtagaaa actgcattct ggttgatgac tcagttgccg gtgcgcaatc
     8881 tggtatcgac gcaggtatgg aagtgttcta cttctgcgcc gacccgcaca ataagccgat
     8941 cgttcacccg aaagtcacca cctttaccca tctttcgcag ttaccggaac tgtggaaagc
     9001 gcgtggttgg gatattacgg catagttctt cacactccct tcacttaccc cgcttaaatt
     9061 ggcgctcaaa ggtaagtaaa gggagtttga tatgtctgtt tcacgtcggg taatacatca
     9121 cggactttat tttgcggttt taggaccgtt aattggtgtt ctgtttcttg tcctctacat
     9181 attcttcgca aaagaaccgc tggttctttt gcgaagaata caggtacttc cattgtttct
     9241 cttattgtcg attactacgg gagttattcc tgcattgtta accggcgtga tggtcgcctg
     9301 tctgccggaa aagatcgggt cacagaaaaa ttatcgttgt ctggctggtg gcataggtgg
     9361 cgtcgttatc accgagatct attgtgcagt tattgtacat attaagggca tggcttcctc
     9421 ggagttgttt gaaaacattc tttctggtga cagtctcgtt gtccgcatca ttcctgcatt
     9481 gctggcaggt gtggtgatga gcagaatcat tacccgtctg cccggattgg atatttcatg
     9541 tcctgaaaca gactctttaa gttaagcggg atactttatc tttgggctac tcaaaagcag
     9601 acaggatgtt actatgactc aaaatatcag gccgttaccc caattcaaat atcatcccaa
     9661 gccactggaa acaggcgcat ttgaacagga taaaaccgta gagtgcgatt gctgtgaaca
     9721 acagacgtca gtttattact cgggtccctt ttattgcgtt gatgaagttg aacatctctg
     9781 tccgtggtgt attgcggacg gttctgctgc tgaaaagttt gcaggtagtt ttcaggatga
     9841 tgccagcata gaaggtgttg aatttgagta tgatgaagag gacgaatttg ccggtattaa
     9901 gaacacatat cctgatgaaa tgctgaaaga gttggttgaa cgcacgccag gttatcatgg
     9961 atggcaacag gaattctggc ttgcgcattg tggcgatttc tgtgctttta tcggctatgt
    10021 gggctggaat gatataaaag atcgcctcga tgaatttgcc aaccttgaag aagattgtga
    10081 gaatttcggt attagaaatt ctgatctagc taaatgcctg caaaagggtg gtgattgtca
    10141 gggttatctc ttccgctgtc tccactgcgg caagctgaga ctgtggggtg atttttcgta
    10201 gttatttaaa taatgagaac aggccggagc gtaattcata catccggcct tatttcttca
    10261 gcttaaccga gcgctaattc tgcggcagcg gctttatcag cgattaccat cagcgaagga
    10321 tgcagctgca acacggaagc tggaacatct tcggtaaccg gaccttgcag aacatttttc
    10381 agtgcctgcg ctttacctgc gccactgaca attatcagca agtttttcgc cgccatgatg
    10441 cttttcggcc ccatcgtcac gtagctgtca ggcactaacg aaaagtcgcc gcccaactca
    10501 ccatgcgcca caatatcaac catctctccc tggatcggga actccacggt ttgctcatgg
    10561 aaatgggtcg tattcggcaa attgccacaa aaatgaccat ctgcacctaa tcccagcacc
    10621 accaaatcca ggccgccttc acgcgccagt ttctgatcat gctcgcggta gttatcaata
    10681 gtgagcttct ggatattctc ttctttgatc cccgcagggg tgaaaaacag attacgcaga
    10741 ttggtaatcg ttacaccttc tccctctttg ccgcgaaagg gaatttcatc gaaattatag
    10801 aaatagcagt tatcgtacca gggcttacct ttaaccaggg tggtgaggta ttcatacatg
    10861 cctttgggcg tgctaccggc ggtaattgcc aggttaacac gacgcgtctt cgacatataa
    10921 ccaagcagat ggtgtgccgc gacacggctc atttcctggt aatcttcggt aatgattaat
    10981 ttcatcttag tgccttattt tatttaaata atttctgtac gaaggctgca taggcaggcc
    11041 gccagacatc catttcatgg ttcaggccgg gatattcctg gtaatcaaac ttaatttttt
    11101 tctgctcaag ctcagttttc agcccggcga tatccttgcc ggttacgaca tctttatcac
    11161 caacgaccac agtaaaatta cgtagttgct ggttaatagc tgccggatcg ttcagccggg
    11221 ccgcgacacc ttcatccggt acggttgtcg tggtaacacc actgaatgtg gccagccagc
    11281 caaagctttc cagatgattc attcccgata ccagcgcctg atacccacct tgtgaaagcc
    11341 ctgccagtgc gcgaccatcg gcatctttac tgacgttaaa acgcttgcta atcagcggga
    11401 taatatcgtt catcagttcg cgatctgccg ctttagcatt cagcggataa aagactttac
    11461 gtctttcctg aggcacgaaa tcttcgggaa taatgccctt cgcatcggtt tcggtatccg
    11521 ggatcaccac cagcatcggt ttaattttcc cttcagccag caggttatcc atgatttgcg
    11581 ggatacgccc ctgatcgata gcggaacgtc cggtatcacc aaaaccgtga tagaaataga
    11641 gcactggcaa aggctcgccc atgccggtgt atcctggcgg ggtccagaca tacatctgac
    11701 gttcagattg caatgcgttg gagtggtaag ttatggcgat caaatcacca tgcgcaacag
    11761 aacgcgtatc cagataactg cctggcacca gaatcatact ggagttaacc tggcgctgag
    11821 gcttagtcat tgcggtgcct gtatcaatgc tgcgtacacc atcaacattg aaaaaatact
    11881 cgtacaggtt gcctttcagg ataggtgtgc gccacgacca gactcctgct tcgtctttgg
    11941 tcatcgggtg aatattgtcc agaaccggaa cacccaccac tacagagaca ttttttgccc
    12001 caggggcaaa gtagcgaaag gtcacgctgt tatcggcatt gacctgagtc acatactgtt
    12061 tcaccgggat agtgggtgcc gggctggctg gcatatccgc tgctatggcc cactgagcag
    12121 aaataccgct ggcaatagcc agcgttaaag cagcaatttt tatattcatc gtaatattcc
    12181 tttcattaag ttccattacc accagatttc agcctgggca ccgacagcaa actggtcgtc
    12241 tttattgtct tcgaaggtga agccatccag ttcgttttcc agggctttga tatacgtggc
    12301 gtagaaacga atttccggac gcgaggtcaa catactggta ttgactttaa aggtatggaa
    12361 gagcgtggtt ttataaccag actcattaaa tttattacta ttcgcatctt tgttttgctg
    12421 ggtgaaatag cccagttcaa cacctgtctg gttatattgg tcccaaatat aggccgggcg
    12481 aacaaccgca cgaatagatt cgaaatcaga gtgtgcgcct gtttcgtagc tataaatatc
    12541 gttaccgaag gagtaaacaa tcgcgttagc cacaatgaaa tgatcgccga tataggcttc
    12601 gccctgtgaa gtcagacgaa ctgctgttcc gccggtgtga tcaccataat aacgaccatt
    12661 aaaggtggta aatggacttg cgccagcata acggccaaag ttactggcga tagagttatt
    12721 cgcaaccagg aaggagaatt cgttgaagcc acctttatca aatttctgcg ttaaagatgt
    12781 gccaaacatc caggtatctt tccagtcata atagccgtta ttatcctgat tatctttttc
    12841 cgatgcgctt tcgtttgccg tgacataacg accactcacc attaaggtcg ctttatccca
    12901 taacgggata tctttatagc gtaaatcaat agcattggta ttaatctgct gtttgttttg
    12961 caggctgcga tcgtaatcat cgacatcttc gcgaaccagc gcgatatcaa ttttacccgg
    13021 accgactttc cagttttcca gacctacacc cgctgcggca tcagtacgct gcgttttcca
    13081 gtcaagcatc tggatttcaa ttttcggcgc accgtgttta cccacccaga aatcagcctc
    13141 tggcgcaaag ggcaggaaac ctttggtggt aacgtacata tcggagaact gcataaagtt
    13201 ctcgccaccg gcgttatcgc caaaccagcc ggtagagtac tgctgaccaa cgttaccatc
    13261 catcatcaca acggcatcaa cccgtttgcc gttttcgttg tagacacgtt gttttaactg
    13321 caaatcaaac cagccggagt attcgttacc aaagcggccc agagaaccaa tcgcccatga
    13381 tttaggtgaa ccatgagagg cggtccccca accagaacgg taatagccgt tgtaactaaa
    13441 gccaatttca tctttcacaa acttactgaa atctttcaga gtcatggaag aataaatcga
    13501 gcctgtctca ctggcatttt tctcttcatt tttgaccagt acagcatcag gttttgcggc
    13561 actactggtc gggaacggat tggcggcata cccttgatca ttcgtactta cgctacgatt
    13621 caccgtcgct ggcgtatatt ttttcttctc ttcatcttta tactttttga gttcgctttg
    13681 cgtttctctt aacgcttttt ctaataactc aaggcgatgt tccaccgtta atgattcagc
    13741 caatgattgt gcagaaaagg ctaacggtgc cattaataag atggcagagg taataagatt
    13801 tcgtctaaac ataatatccc tttatggtgc aaagaaagaa ttaacgtatc gcatccagac
    13861 tgttctgaat gcgacgataa ttaaggtgct ttattggtta tttttttaat gacagccccc
    13921 gcgttttgct cacttctgca taccatccga agcttttctt acgcgttctt gtgaggcttc
    13981 cttcgccatt atcatcacga tccacataaa taaagccgta gcgcttagac atttgtgaat
    14041 gagatgcact gactaaatca attggccccc aactggtgta ccccataata tccacaccat
    14101 cggcaatcgc ttcatttacc tgtaccaggt gatcgtttaa ataggcaatt cgataatcgt
    14161 cctgtatcga accatccgct tcaacgctgt cttttgcgcc taatccgttc tcgacaataa
    14221 ataacggttt ttgataacga tcccaaagcg tatttaacag aacccgtaat ccaaccggat
    14281 caatttgcca cccccactct gaacttttca gatgcggatt ggggatcata ttcagtatgt
    14341 tgccctgcgc atttttatta atgctttcgt cgtgggaaac acaaccagtc atgtaataac
    14401 taaaagagat gaaatctacg gtatgtttta aatcttctgc gtcactttca gtcatctcaa
    14461 tggtgatatt gtggtcgcgg aagaaacgct gcatatagcc gggatactga ccacgcgcct
    14521 gaacatcacc aaagaacatc cagcgccggt tctcttccat ggcctccaac atatcctgtg
    14581 gctggcaggt gagggggtaa accagcccgc cgagaagcat attgccgatt ttcgcttcgg
    14641 ggatcaggct atgacaggct ttaactgccc gcgcactggc aaccagttga tgatggatcg
    14701 cctgataaac ttccgcctcg ccactctctt ctgccagccc cacgcccgtg aatggcgcgt
    14761 gtaacgacat attgatttca ttaaacgtca gccataacgc tactttatgt tggtagcggg
    14821 taaagaccgt gcgggcgtaa tgctcgaagt gatcgatgac cgcccgatta gcccaaccgc
    14881 cgtagttttt caccagccca tatggcattt cgtaatggga taacgttacc agcggcttga
    14941 tccccgcctg cgccatttca tcaaacagcc gatcgtaaaa cgctaacccc gcttcattcg
    15001 gttcggcttc gtcgccctga gggaaaattc gcgcccaggc aatggaaata cgcagacagg
    15061 tgaagcccat ctcggcaaat aacgcgatat cttccgggta acggtgataa aaatcgatgg
    15121 cgacatcttt gatattctct ttccccagga tgcgcggttc catttttccc attacgccat
    15181 gaggctgtaa atctgaggtc gtgatccctt tgccatcttc cagccaggca ccttccacct
    15241 gattggcagt tgttgcgcca ccccaaagaa atgtttctgg aaatgctttc ataattaact
    15301 ccttttatcg ttagcgaatg atggataaca gcggctcacc agcgcttatc tgcgccgtgc
    15361 cgtggggtaa tacgtctgta aaatcatcgc tattactgat taataccggc gtcgtcagat
    15421 caaatccggc ctcgcgaata gcagggatat caaaagaaat cagccgatcg cctgtattga
    15481 ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc gtccagtttt acggtgtcga
    15541 taccgacatg aatcaggatc tccacaccat catctgactc aatgccaata gcgtgtaatg
    15601 tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg cacttcacca accgagggca
    15661 gaatggcgat acctttaccc aacaggccac tggcaaacgt ggtatcagcg acgtgaatga
    15721 gcggcacaat ctctcccgtc atcggtgaac agataccgcc ctgctcaggt ggtgtaataa
    15781 cctctggtgt tttctcttgc ggggcaccct gcgctggctg acgtttagcg gtgatgaaat
    15841 gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat gacaccgcca ataacgctgg
    15901 cccagacggt gaaatcaatc cccgttgacg ggatggtttg catgaaggtg aaaatacttg
    15961 gcaaaccaaa ggagtaaact ttcgtttgcg cgtagccaat gatggtggcc cccaatgctc
    16021 cactgataca ggcgataacg aagggatact tacgcggcag gttgacgcca tataccgctg
    16081 gttcggtgat accaaacagg cccgtcaacg ccgctgatcc cgccaccact tttttctgcg
    16141 catcgcgttc gcagaggaag acgccgagcg ccgccccgac ttgcgccata atggcgggca
    16201 ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa gttattgata cacagcggca
    16261 ccaggcccca gtgcagtccg aacatgacga agatttgcca gaaaccgccc attaccgcgc
    16321 ccgcaaatgc aggaaccgcc tgataaagcc agagataacc ggcggcaatc atttcgctta
    16381 tccaggttga tagcggcccc accagcaaaa aggtgacggg tgtgataacc atcagacata
    16441 gcaatggtgt gaagaaattt ttgattgccg acggtaacca cgcattaagt cggcgttcca
    16501 gaatgctgca caaccaggca gaaaaaataa tgggaataac cgatgacgag taattcaaca
    16561 atgtgaccgg aatacccagg aaatccagcc ccagctcatc cgctttttgc ccgttctcga
    16621 aagcagtcag aattaatgga tgcactaacg ctccaccaat caccatggca gtaaatggat
    16681 taccgccgaa gcgtttcccc gcggtgtatc ccaggattat cgggaagaac caaaacaagg
    16741 catcactggc gctgaataaa attaaataag taccactttg ttcggtcgtc cactgaaaag
    16801 tgagcgccag agccagcata cctttcagga tcccggttgc cgccatcaaa ccgatcagag
    16861 gcgtaaaaat acctgaaata acataaacaa agcggtttag cagattacct ttatcatcat
    16921 tttccggtgc ctgttgcgct ttttcgtcaa ggcctgccac actgttaacc gccaggaaga
    16981 catcggccac atggttacct atgaccacct gaaactggcc accgctttcc accaccataa
    17041 taataccggg ggtctttttc agtacctctg cttgcgcttt gctttcatcc tttaacttaa
    17101 aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt atctgcgccc ccgactcctg
    17161 cgactatttt tctggctaac tccgtcataa cttgccctct accgctttgc ggcaaaactc
    17221 aaaaaaaaaa cctgaaaaaa acggcctgac gtgaatcaag caattttttc aggttttgcc
    17281 cgcttagtgc ggtaacaatc ctttactcag taataatatt tcagtgttct ttgcgcacgc
    17341 gctctatatt tatggctaaa aacataatct ctgcgggtga gattttacgt tgatactgca
    17401 aaccaataaa aatggcgatc cgctccgcac attgccatgc ttgcgggtaa ttttgtttta
    17461 ctgcttgttg taatgactca tcactatcgt taattgaagc atgtccaaga atacgccagg
    17521 ataaaaactt cagatgcgta accagtcgct gataactcaa gctttcttcc tggtaattaa
    17581 ggctgaactg aaattttatt aattgcagca tttcgcgcat taattgcgtg acacctgcaa
    17641 catcctccat atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt
    17701 catctttcgg taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta
    17761 gctggaactc tttcgggtaa agccgctgga tatcccacag cagcgggttg ggcagtaaca
    17821 cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac
    17881 tgtcctgtaa ttttcccaag cgctcctgcg ctaaagagat aatacgatca caggttgcca
    17941 tcacctcaag aggaatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc
    18001 tcaaggcata ctctttttct attccacttg agttaattct ttcgccagcg cgtttttgaa
    18061 agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa
    18121 cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca
    18181 aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
    18241 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
    18301 attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta
    18361 ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc aataattaag
    18421 ttattgggat ttgtctggtg aattatttgt cgctatcttt ccccgccagc agtttatcca
    18481 gctcatcgcc accgacgtga cggaaatcct gccccttcac gtagtagaag ataaactcac
    18541 aaatattctg gcagcggtcg ccaatacgtt cgatagaacg cgcgcagaac agcgcagtaa
    18601 gtacgctcgg aatggtacgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
    18661 cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
    18721 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
    18781 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
    18841 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
    18901 taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
    18961 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca
    19021 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
    19081 cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa
    19141 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
    19201 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
    19261 ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg
    19321 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
    19381 aatacgcccg gtagagatag ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc
    19441 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
    19501 gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
    19561 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa
    19621 agcgatgttg tcgtagatgg acatcggaaa cggcgtcggt ttctggaaca ccatgcccac
    19681 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag
    19741 aatttcacct tctgcgcgct gctccggata cagttcaaac attttgttga aggtacgcag
    19801 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat
    19861 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
    19921 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct
    19981 gatgcttttc gctaatcagg cttaccgtag gccgggtaag gcgttcacgc cgcatccggc
    20041 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
    20101 gacgcacagg gtaatgatca ataccccggc ccaggccagt tgttgccatt ccgcaaacgg
    20161 gctcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
    20221 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg
    20281 ggcaatcgcc agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc
    20341 ggagatcatc ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg
    20401 caccagtttc agcatgttct cggtggtgcg gataacgatc ggcacctgca acagcgccag
    20461 cgcaattacg cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac
    20521 aaacagacca accacaatcg acggcgcgga gagcagaatg tcgttaatga agcgaatcac
    20581 ttctgccagc caggatttac gaccatattc cgccagataa atccccgcca taatgcccag
    20641 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
    20701 cagaccacca ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat
    20761 accgtcgata ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa
    20821 ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg
    20881 cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag
    20941 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
    21001 aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat
    21061 tccgcttccg caaattcgtt cgccagcgca gaggtgatac tgttgcccgg catatacagc
    21121 gaggcgctgt cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
    21181 agcgcgcggc ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga
    21241 acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
    21301 atcaccgggg tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg
    21361 atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc
    21421 gcgccaacaa tcgggatatt cgacatgata ttgccgaccg gctcctggaa gtaaacggcg
    21481 aatagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc
    21541 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg
    21601 aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc
    21661 agcgtaccgt agatcggcac tagcgccccg tagatatcgt tcggtgcatc ccactctttg
    21721 gtccagagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
    21781 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg
    21841 aaaattatgt cgcccttttt acccggtggg ttaaaagcag gcttggttgc agccataagt
    21901 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggataag ccgttaatgc
    21961 cttatccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
    22021 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
    22081 gttcgcctgt ttagcaccgg ttttgtacgc ccagtcgaag aatttcagca cttctgtgcc
    22141 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
    22201 cgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcgcc
    22261 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
    22321 cagtttggta tacgccaggt tgttctgctt cgcgtaagca tattcaacgt aaccaattgc
    22381 acccggcaga cgctgaacga acgcggcgat accgtcatta cctttaccgc ccagaccgat
    22441 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
    22501 cagatagctg gtgaagacga aggaggtacc ggagccatct gcgcggcgta ccacggcgat
    22561 gttctgtgac ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
    22621 cttgattttg ccgaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
    22681 cttcagccct ggaatgttaa cagccagcac cacgccgcca atcacggtcg ggaactggaa
    22741 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
    22801 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
    22861 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
    22921 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcgctca tcgataaggt
    22981 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
    23041 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
    23101 tgtaaggaat atgacagttt tatgacagag agataaagtc ttcagtctga tttaaataag
    23161 cgttgatatt cagtcaatta caaacattaa taacgaagag atgacagaaa aattttcatt
    23221 ctgtgacaga gaaaaagtag ccgaagatga cggtttgtca catggagttg gcaggatgtt
    23281 tgattaaaaa cataacagga agaaaaatgc cccgcttacg cagggcatcc atttattact
    23341 caaccgtaac cgattttgcc aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg
    23401 cgacatggta agccagcagc tgcagcggaa cggtgtagaa gatcggtgca atcacctctt
    23461 ccacatgcgg catctcgatg atgtgcatgt tatcgctact tacaaaaccc gcatcctgat
    23521 cggcgaagac atacaactga ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt
    23581 tttccaacag ttcgttgttc ggtgcaacga cgataaccgg catatccgcg tcaatcagcg
    23641 ccagcgggcc gtgtttcagc tcgcctgcag cgtaggcttc agcgtgaatg taagagatct
    23701 ctttcagttt caatgcgcct tccagcgcga ttgggtactg atcgccacgg cccaggaaca
    23761 gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc aatgcgtttg tcctgagaca
    23821 gcatctgctc aatacggctc ggcagcgcct gcagaccatg cacgatgtca tgttcaatgg
    23881 aggcatccag acctttcagg cgagacagct tcgccaccag catcaacagc acagttaact
    23941 gagtggtgaa tgctttagtg gatgccacgc cgatttccgt acccgcgttg gtcatcagcg
    24001 ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt acagattgcc agcgaaccaa
    24061 ggtaacccag ctctttcgac agacgcaggc ccgccagggt atccgcggtt tcgccagact
    24121 gtgacaaggt gatcatcagg ctgttacgac gcacggcaga tttgcgatag cggaattcag
    24181 aggcgatttc gacgtcgcac ggaatacctg ccagcgattc aaaccagtaa cgggaaacca
    24241 taccggagtt ataagaagta ccacaggcga ggatctgaat atgctcaacc ttcgacagca
    24301 gttcgtcggc gttcggtccc agctcgctta aatcaacctg accgtggctg atgcgcccgg
    24361 taagggtgtt tttgatcgcg ttcggctgtt cgtagatctc tttctgcatg tagtgacggt
    24421 aaataccttt atcgcccgcg tcatattgca gattggattc gatatcctga cgttttactt
    24481 ccgcgccagt tttatcgaag atgtttaccg aacggcgagt aatttccgca atatcgccct
    24541 cttcaaggaa gataaagcga cgggtcaccg gcaacagcgc cagctggtca gaagcgataa
    24601 agttttcgcc catacccagg ccaatcacca gcggactacc agaacgtgcc gccagcaggg
    24661 tatccgggtg acgggagtcc atgatcactg taccgtacgc accacgcagt tgcgggatag
    24721 cacgcagaac agcctcacgc agagtaccgc cctgtttcag ctcccagttc accagatggg
    24781 caattacttc ggtgtctgtt tcagaaacga aggtatagcc acgcgctttt aactcttcgc
    24841 gcagcggttc atggttttcg atgatgccgt tatgcaccac cacaatgtgt tcagaaacat
    24901 gcggatgcgc attcacttct gaaggttcac cgtgggtcgc ccagcgagta tgagcaatac
    24961 ccgtaccgcc atgcagagga tgttcttccg ctgcctgagc cagcatctgg actttaccga
    25021 ggcgacgcag gcgggtcata tgaccttccg catcaacaac ggccagaccg gcagagtcat
    25081 atccgcggta ttccagacga cgtaaacctt caagaaggat ttctgctaca tcacgttgcg
    25141 cgatcgcgcc aacaattcca cacatagttt ttgattccga tttatatcgt tgtcggtcaa
    25201 cctgtatgcc cgttttttcg ggcgccccga gccttgtaga gagtggggtt atttttatag
    25261 ttactgcttg tgggagggag attatgttat ctcctcatcc catgtgaccg gattacccgg
    25321 ccagaatcac tttttcttta ccggacgacg ccagccttct ttctgagtct gcggcacacg
    25381 gctgatagct aatgcatttt cgccgacatt acgcgtcaca gttgtacccg cagcaatggt
    25441 cgcgcctttg cctactgtta ccggggccac cagctgagtg tcggaaccga caaacacatc
    25501 gtcgccgata atggtcttaa atttattcgc accatcgtag ttgcaggtaa tggttcccgc
    25561 gccgatgtta acgttatcgc caatttccgc atcgcccagg taagtcagat gaccagcttt
    25621 cgagccttta cccagacgcg cttttttcat ctcaacaaag ttaccgacgt gtgcaccttc
    25681 cagcaactca gcaccaggac gcagtcgggc aaacgggcca atagtacagg ccgctgccag
    25741 attcgcgtct tccacaacgg tatacggact gatttcgcaa tcatcgccaa tcacgctgtt
    25801 tttaatcacg caaccggtgc cgattttcac gcgatgaccg agagtcacgt tgccctcgat
    25861 gataacgtta gtatcaattt caacatcgcg cccgtgagta agcgtaccgc gcaaatcaaa
    25921 acgcgccgga tcgcgcagca taacgcctgc taacagcagt ttttcagcct gttcggactg
    25981 gtaaacgcgc tccagacggg agagttgcag gcggttattc acaccttcta cttcgcttaa
    26041 acgttgcgga tgaacggcga cgatttcacg cccttcctga tacgccagcg caataatgtc
    26101 ggtgatgtag tattcgccct gagcattatt gttggtcagc ttcgccagcc agcgtttcat
    26161 atctgcgccg ttagcgatca gaatgccggt gttgatctcc tgaatctgac gctgctcgtc
    26221 ggtggcatct ttgtgctcaa caatgccggt aactttgcca ttttcacggg tgatacgtcc
    26281 ataaccggtc ggatcatcca gtttcaccgt cagcagacca atgccaccct gcggtttagc
    26341 atcacgcagg cgctggagtg tttcgacaga gatcagcggc acgtcgccgt agagcattaa
    26401 aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc attgcatgac ccgtacccag
    26461 ctgctctgcc tgaagcaccc agttaaggtt gtcgtctttc agcgcctgtt taagcagatc
    26521 gccgccgtga ccgtacacca ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat
    26581 gacatgctga accatcgctt tcccggcaag ggtatgcagc actttcggaa gatcggaata
    26641 catgcgcgtg cctttgcctg cggcaaggat cactacgctc atagcattat tcaacatacg
    26701 cgtcctgact gtaatttgag aacgaattta aaccgcttca ccttgaaaaa actacatttt
    26761 tttcatcgtg aaatggacag aggataaatt gttcaataac ggattatccc cgctgacgaa
    26821 agtgccattt tcgaccatcg tcacgccttt tgtctgcaga aaattaaggc agtaccttca
    26881 tttaagcgtc aggtggatgt ttttgctctt atttcgatca atgaataaac gggaaaacag
    26941 gggtgtttta tatcagcagg atctatgtga acgctcttca ggacggatca cgcgtaaaaa
    27001 aaagccagcc tgtttccaga ctggcttttg tgcttttcaa gccggtgtta catcgctttt
    27061 ttggtcaact cgataacgcg cagctgcgcg atcgctttgg ccagttccgc agacgcctga
    27121 gcgtaatcta cgtcgccgtg agagctgcta atgtgctctt cagccttacg tttcgcttcc
    27181 atggctcgcg cttcgtcgag atcctggccg cgaattgcgg tgtcggccag aacggtcacg
    27241 ttgccaggct gcacttcaag aatgccgcca gacagataga taaactcttc gtgaccgtgc
    27301 tgtttcacga tgcgaatcat accaggctta atggcggtga gcagcggtgc gtggccaggg
    27361 tagatcccca gttcaccttc gctacccgtt acctggattt tctcgaccag accagagaac
    27421 atttgttgct ctgcgctgac gacgtccagg tggtaagtca ttgccatatc accctccgat
    27481 taaggcgtta aagttttttg gctttttcca cagcttcttc gatggaaccg accatgtaga
    27541 acgcctgctc cggcaggtga tcgtattcgc cttccatgat gcctttaaag ccacggatgg
    27601 tgtctttcag ggagacgtat ttacccggag aaccggtgaa tacttctgcc acgaagaacg
    27661 gctgggacag gaagcgctgg atcttacgag cacgcgctac caccagtttg tcttcttcag
    27721 acagttcatc catacccagg atggcgatga tgtctttcag ttcctgataa cgttgcagga
    27781 tggactgaac gccacgcgcg gtgtcgtagt gttcctgacc aaccaccagc gggtccagct
    27841 gacggctggt ggagtccagc gggtcaacgg ccgggtagat acccagagac gcgatctgac
    27901 ggctcagtac cacggttgcg tcaaggtgcg caaaggtggt tgccggagac gggtcagtca
    27961 agtcatccgc aggtacgtat actgcctgta cggaggtgat agaaccagtt ttggtggagg
    28021 tgatacgttc ctgcagaacg cccatctctt ccgccagggt cggctgataa cctaccgctg
    28081 aaggcatacg gcccagcagt gcggatactt ccgtacctgc cagggtgtaa cgatagatgt
    28141 tgtcaacgaa cagcagaacg tcacgacctt cgtcacggaa tttctcagcc atggtcagac
    28201 cggtcagagc aacgcgcaga cggtttcccg gcggctcgtt catctggcca tacaccaggg
    28261 atactttgtc gataacgttg gagtcggtca tttcgtggta gaagtcgtta ccctcacgag
    28321 tacgttcacc tacgcccgca aacacagagt aaccggagtg ctcgatcgcg atgttacgaa
    28381 tgagctccat catgtttacg gttttaccta cacccgcacc accgaacaga ccaactttac
    28441 cgcccttagc gaacggacac atcaggtcga taactttgat accggtttcc agcagttcct
    28501 gagagtttga cagctcttcg taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt
    28561 caccgatctc gcctttcatg tcgaccggtt cacccagtac gttcatgata cggcccagag
    28621 tcgctttacc taccgggact tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac
    28681 gcagaccgtc ggaggaaccc attgcgatgg tacgcacgat accgccgccg agctgctgct
    28741 gaacttccag caccagacgc tcattaccat tttgcacctc aagagcatcg tacacgcgcg
    28801 gtacggcatc ctgagggaat tcgacgtcaa ctacggcgcc gattacctgg acaatctttc
    28861 cagtagccat cttaaatcct ctacgaaata acctgtttaa accgcggcgg cccccgagac
    28921 gatctcggtg agttcctgag taatgctggc ctgacgagct ttgttgtata ccaactgcag
    28981 ctctttaatc aggctgccgc cattgtcggt cgcggctttc atcgccacca tacgggcggc
    29041 ctgctcgctg gccaggtttt caaccacgcc ctgataaacc tgagattcga cataacgacg
    29101 cagcagggta tccagcaacg ccttcggatc gggttcgtac aggtaatccc aggatttatg
    29161 tttcagatca tcatcatctg atgccggtaa cggcagcagc tggctgatgg tcggaacctg
    29221 agacatggtg ttaataaatt tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc
    29281 gtaggcctgc aacatcactt ttaccggacc gatcagttcg gacagggaag ggttatcccc
    29341 catgccggtg acctgggcaa caacattgcc gcccacggag ttgaagaacg acacgccttt
    29401 cgagccgatc attgcgaggt cgcattgaac gcctttgtcg gtccaggtct tcatttccgc
    29461 cagcagtttt ttgaacaggt taatgttcaa accaccgcac aaaccacggt cggtcgacac
    29521 caccaggtag cccacgcgtt taacgtcgcg gtcttccagg taagggtgct tatattccag
    29581 attaccgtgt gcaaggtgac caatcacttt gcgcatggtt tctgcataag gacggctggc
    29641 cgccatgcga tcctgcgatt tacgcatttt ggaagcggcg accatctcca tcgctttagt
    29701 gatcttttgc gtgttctgga cgcttgcgat cttactacgt atctcttttg cgccggccat
    29761 gagcttctcc tcaatgcctt gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat
    29821 tgggttgctt tgaaggaatc gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag
    29881 ccaccggtct ggttgatctc ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc
    29941 agagcggctt cgaagctgcc aattttcgac agttcaacat ccgccaggta accacgttct
    30001 gctgcgaaca gaaccagaga ctgctgcgca acggacatcg gcgcatactg tttctgtttc
    30061 agcagttcgg tcactttctg accgtggtca agctgcttac gtgttgcatc gtcaaggtcg
    30121 gatgcaaact gagagaacgc tgccagttca cgatactgtg ccagagcggt acggatacca
    30181 ccggacagtt ttttcatgat cttggtctgt gctgcaccac caacacggga tacggaaata
    30241 cccgggttaa ccgcaggacg aataccggcg ttgaacaggt tggtttccag gaagatctga
    30301 ccatcggtaa tggagattac gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt
    30361 tcgataatcg gcagtgcggt cagagaaccg gttttccctt tcacttcacc tttggtgaag
    30421 gcttcaacgt attcggcgtt aacacgtgca gcacgctcca gcagacgaga gtggaggtag
    30481 aaaacgtcgc ccgggaatgc ttcacgtcct ggcggacgac ggagcagcag ggagatctga
    30541 cggtaagcaa cagcctgttt agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg
    30601 tcacggaagt attcgcccat tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg
    30661 gattcagacg cggttgctac cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt
    30721 ttacgtacca cgttagaaat ggtggacgct ttctggccga tagcgacata gatacatttg
    30781 ataccggaat cgcgctggtt gatgatggca tcgatagcca gtgcggtttt acctgtctga
    30841 cggtcaccga tgatcaattc acgctgacca cgaccgattg ggatcatgga gtcaacggct
    30901 ttataaccgg tctgtaccgg ctgatctacg gactgacgtt cgataacgcc cggagcgatt
    30961 gcttctacag cagagaagcc gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc
    31021 agagtgttaa ccacacggcc cagcaggcca cggccaaccg gaacttccag gatacggcca
    31081 gtacacttaa ctttcatgcc ttcggcaagg tcagcgtacg gacccataac aaccgcacct
    31141 acagagtcgc gctcgaggtt cagtgcgata gcgtaacggt tacccggcag ggagatcatt
    31201 tcaccctgca tacaatcggc caggccgtga atgcggataa caccgtcact tacagaaaca
    31261 atagtacctt cgttgtgagc ttcactcaca acattgaact gagcaatgcg ctgcttgatc
    31321 agttcgctga tttcggtgga attcagttgc atgctccagt ccccttaaga ctgcaagacg
    31381 tctgcaaggc gctcaagacg accgcgtacg ctgccatcaa tgaccatatc acccgctcgg
    31441 atgataacgc ctgccattac agacttatcg attttgcaat tcagcttaac tttgcgtgac
    31501 agacgttttt ccatcgcagc agaaattttc gcgagctgtt gttcactcag tgcggcagcg
    31561 gaaatgacgt ctacctcagc ggtagcctca ctcacggcac gaaggtgaat aaactgctcc
    31621 agaacatccg ggagcgcgtt aagacgacca ttttcagcca ttacccgaat caggttctga
    31681 ccgttttcgt ccagttgctc accacaaact gcgataaacg actcggcgag cgtttctggc
    31741 gcaagcgcgc cagagagaag ctctgccatt tgttcgtttt tggttacctc ggcggcaaac
    31801 gccagcatgt cctgccagcg ttctacactt tggtgttcga cggcaaagtc aaaagctgct
    31861 ttggcgtagg ggcgagctac cgtaataaat tcagacatca gcccctccct ccttacagtt
    31921 cagcgacaag tttatccacg atgtcgctgt tagcagcttc atccacggaa cgttcgatga
    31981 tcttctcggc gccagcaaca gccaggatag caacttgctt acgcagctct tcacgggcac
    32041 gtttacgctc ggcttcaatt tctgcctgcg cctgggccac gattttagta cgttcctgtt
    32101 ctgcctcagc tttcgcttcg tccagaatct gcgagcggcg tttgttcgcc tgctcgatga
    32161 ttacctgggc ttccgctttc gcttttttca gctggtcggt cgcgctggcc tttgcaaggt
    32221 caaggtcctt atgtgctcgt tctgcggaag caaggccgtc agcaatttct ttttgacgtt
    32281 tttcgatggc tgccattaat ggcggccata cgtacttcat gcagaacaga acgaacagga
    32341 caaacgcgat ggcctggccg aggattgttg cgttaagatt cacagcacaa tgcctctatt
    32401 tagttaacgt tctgatattg ctctttaaat aaaagcaacg cttactacgc gacagcgaac
    32461 atcacgtaca gacccagacc tacagcgatc atcgggatag catccaccag acccataacg
    32521 ataaagaact gagtacgcag cagaggaatc agatcaggtt gacgcgctgc gccttccagg
    32581 aatttacccc cgaggatgcc gataccgatc gcagcaccga ttgccgccag acccatcatc
    32641 acagcggcag ccatgtacag cagatccata ttcaggtttt ccatgacagt ctccagtttg
    32701 tttcagttaa aacgtagtag tgttggtaaa ttaatgttct tcagacgcca tcgacagata
    32761 gacgatcgtc agaaccatga agatgaaggc ttgcagcgta atgatcagga tgtggaaaat
    32821 ggcccacggc acattcagga tccactgtga ccaccacggc aacagaccag caatcagaat
    32881 gaaaatcagc tcaccggcat acatgttacc gaacagtcgc aaaccaagtg aaactggttt
    32941 ggacagcagg cttacccctt caaggattaa gttgacagga atgaacgccc agtgattgaa
    33001 cggctgcagc gtcaactctt tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa
    33061 cagaatcagg ataaatacgc ccaatgccat agacagcgtt acgttcacgt ccgcagacgg
    33121 aaccacacgc agtgcaggca gacccagtac atgttcagca atgtacggca gcaggtcgat
    33181 aggcagtaaa tccatcaggt tcatcaggaa tacccagacg aagatcgtca gggccagcgg
    33241 agcaatcagc ttgcttttgc catggtacat gtctttcacg ctaccattaa caaagccgat
    33301 caccagctca atcgcggtct gaaacttacc tggcacaccg ctggtcgcct ttttggctac
    33361 gctacggaat aaaaccagga acaacagacc cagcaccacc gagaagaaca tggagtcaat
    33421 attgattgtc cagaaggtgg ctggggggtt ttgtggatcc accagcgaga atgtacgcag
    33481 gtccagctga aggttattca ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga
    33541 agccatgatg ccttttaccc tttgttgtta attacagccg gtgccagtat ctgaaccacc
    33601 agcaccaaaa cccacgtaac gatcagcggc aagaataccg cctttaaaac cgccaacgcc
    33661 accaccagta acaccaacat cgccagaact ttgaaagctt cgccaaatgc gaatgtccag
    33721 gccacccggc ctttcgctgg tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa
    33781 acgttaggca gaaagactgc caggcccccg cttattgcag agacgcccca gaaggggtct
    33841 ttgaggctga acagcaatcc acttgctatc accaccagta actgaacgag cagaagcttc
    33901 cgagcaacgt ttcgactcac gagcgacaca gacatcacgt ttttcactcc tgctcccttc
    33961 gaggtatgcc gcgtgtcgta taaaactttc tttaaggctt agagtcaagc atcaaaaagc
    34021 ggtcaaatta tacggtgcgc ccccgtgatt tcaaacaata agtagccaaa agatgaataa
    34081 atgtttaaat atttttccag tgcatacaat tgcgactttt ctgctaaccc tgttcgatca
    34141 tgaaaaactg taaataacgc gtaaacactg gtgataaagc gtgcttcaga tcacatattg
    34201 cgcatgttcg cgcacagcat atttatttac
//
LOCUS       DAZGCS010000049        33999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGCS010000049 DAZGCS010000000
VERSION     DAZGCS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.049"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..285
                     /locus_tag="ACHK01_002430"
     CDS             <1..285
                     /locus_tag="ACHK01_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097374.1"
                     /translation="VLDTDPEKRINEVKNFLTALSDSICSLFIVNSNVASISLGNESF
                     STDEDLEYGYLINTGNHYDVYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
     gene            578..1819
                     /gene="agp"
                     /locus_tag="ACHK01_002431"
     CDS             578..1819
                     /gene="agp"
                     /locus_tag="ACHK01_002431"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO8097375.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(1857..2084)
                     /gene="yccJ"
                     /locus_tag="ACHK01_002432"
     CDS             complement(1857..2084)
                     /gene="yccJ"
                     /locus_tag="ACHK01_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO8097376.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2105..2701)
                     /gene="wrbA"
                     /locus_tag="ACHK01_002433"
     CDS             complement(2105..2701)
                     /gene="wrbA"
                     /locus_tag="ACHK01_002433"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8097377.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPIATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3074..3247
                     /gene="ymdF"
                     /locus_tag="ACHK01_002434"
     CDS             3074..3247
                     /gene="ymdF"
                     /locus_tag="ACHK01_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8097378.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(3330..4658)
                     /gene="rutG"
                     /locus_tag="ACHK01_002435"
     CDS             complement(3330..4658)
                     /gene="rutG"
                     /locus_tag="ACHK01_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO8097379.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(4679..5173)
                     /gene="rutF"
                     /locus_tag="ACHK01_002436"
     CDS             complement(4679..5173)
                     /gene="rutF"
                     /locus_tag="ACHK01_002436"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO8097380.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(5184..5774)
                     /gene="rutE"
                     /locus_tag="ACHK01_002437"
     CDS             complement(5184..5774)
                     /gene="rutE"
                     /locus_tag="ACHK01_002437"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO8097381.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HSDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(5784..6584)
                     /gene="rutD"
                     /locus_tag="ACHK01_002438"
     CDS             complement(5784..6584)
                     /gene="rutD"
                     /locus_tag="ACHK01_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO8097382.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLQQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(6592..6978)
                     /gene="rutC"
                     /locus_tag="ACHK01_002439"
     CDS             complement(6592..6978)
                     /gene="rutC"
                     /locus_tag="ACHK01_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO8097383.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(6990..7682)
                     /gene="rutB"
                     /locus_tag="ACHK01_002440"
     CDS             complement(6990..7682)
                     /gene="rutB"
                     /locus_tag="ACHK01_002440"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO8097384.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(7682..8773)
                     /gene="rutA"
                     /locus_tag="ACHK01_002441"
     CDS             complement(7682..8773)
                     /gene="rutA"
                     /locus_tag="ACHK01_002441"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO8097385.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            9061..9699
                     /gene="rutR"
                     /locus_tag="ACHK01_002442"
     CDS             9061..9699
                     /gene="rutR"
                     /locus_tag="ACHK01_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO8097386.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(9739..13701)
                     /gene="putA"
                     /locus_tag="ACHK01_002443"
     CDS             complement(9739..13701)
                     /gene="putA"
                     /locus_tag="ACHK01_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8097387.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLARRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            13756..13965
                     /locus_tag="ACHK01_002444"
     CDS             13756..13965
                     /locus_tag="ACHK01_002444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097388.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            14124..15632
                     /gene="putP"
                     /locus_tag="ACHK01_002445"
     CDS             14124..15632
                     /gene="putP"
                     /locus_tag="ACHK01_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO8097389.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            16175..17005
                     /gene="efeU"
                     /locus_tag="ACHK01_002446"
     CDS             16175..17005
                     /gene="efeU"
                     /locus_tag="ACHK01_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO8097390.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            17063..18190
                     /gene="efeO"
                     /locus_tag="ACHK01_002447"
     CDS             17063..18190
                     /gene="efeO"
                     /locus_tag="ACHK01_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO8097391.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            18196..19467
                     /gene="efeB"
                     /locus_tag="ACHK01_002448"
     CDS             18196..19467
                     /gene="efeB"
                     /locus_tag="ACHK01_002448"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO8097392.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            20086..20874
                     /gene="phoH"
                     /locus_tag="ACHK01_002449"
                     /pseudo
     CDS             20086..20874
                     /gene="phoH"
                     /locus_tag="ACHK01_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
     gene            complement(20924..21337)
                     /gene="pgaD"
                     /locus_tag="ACHK01_002450"
     CDS             complement(20924..21337)
                     /gene="pgaD"
                     /locus_tag="ACHK01_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO8097393.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(21339..22664)
                     /gene="pgaC"
                     /locus_tag="ACHK01_002451"
     CDS             complement(21339..22664)
                     /gene="pgaC"
                     /locus_tag="ACHK01_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO8097394.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(22657..24675)
                     /gene="pgaB"
                     /locus_tag="ACHK01_002452"
     CDS             complement(22657..24675)
                     /gene="pgaB"
                     /locus_tag="ACHK01_002452"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO8097395.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            complement(24684..27107)
                     /gene="pgaA"
                     /locus_tag="ACHK01_002453"
     CDS             complement(24684..27107)
                     /gene="pgaA"
                     /locus_tag="ACHK01_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO8097396.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            27694..29052
                     /gene="dgcT"
                     /locus_tag="ACHK01_002454"
     CDS             27694..29052
                     /gene="dgcT"
                     /locus_tag="ACHK01_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8097397.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFW
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            complement(29093..30255)
                     /locus_tag="ACHK01_002455"
                     /pseudo
     CDS             complement(29093..30255)
                     /locus_tag="ACHK01_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            30322..30849
                     /locus_tag="ACHK01_002456"
                     /pseudo
     CDS             30322..30849
                     /locus_tag="ACHK01_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            30846..31832
                     /gene="ycdU"
                     /locus_tag="ACHK01_002457"
     CDS             30846..31832
                     /gene="ycdU"
                     /locus_tag="ACHK01_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO8097398.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            32199..33401
                     /locus_tag="ACHK01_002458"
     CDS             32199..33401
                     /locus_tag="ACHK01_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8097399.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(33588..>33999)
                     /locus_tag="ACHK01_002459"
     CDS             complement(33588..>33999)
                     /locus_tag="ACHK01_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097400.1"
                     /translation="FLYNSMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINDII
                     DSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEE
                     KVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT         8388 a         8252 c         8655 g         8704 t
ORIGIN      
        1 gttttagaca ccgacccaga gaagaggatt aatgaggtga aaaatttcct cactgctctt
       61 tcagatagca tttgctcatt atttattgtt aatagtaatg tggcatcaat ctccttaggg
      121 aatgaatcct tttcaacaga tgaagatctt gagtatggtt atttaataaa cactggcaat
      181 cattatgatg tttacctccc tcctgaactt tttgctcagg cttacaagtt aaaaaataag
      241 gaaatgaatg cgcaactcga ctatttaaat cgttatgcaa tttaatggca aaggcatatg
      301 ctaaaaacca ttgttattag tctcacactt ttttattggt aaatattgtc tctgtattgg
      361 taacgccgca gatattctgt ttagccacag gtgcaattat cagcggcgta cgcgaggcag
      421 gggctaatca ggcatagttt gcgtcaaacc ttgcctgttt ttgaagatgt atatagaaaa
      481 acaggcgttc aacaagccat tttgcgaacc tgttcccgga aaaaagtcat atttctgtca
      541 cactctttag tgattgataa caaaagaggt gccaggaatg aacaaaacgc taatcgccgc
      601 agctgtggca gggatagttt tactcgcttc aaacgctcag gcacaaaccg taccggaagg
      661 ctatcagcta cagcaagtgc tcatgatgag ccgccataac ttacgtgcgc cgctggcgaa
      721 caatggcagt gtgctggagc agtcgacgcc gaataaatgg ccagaatggg acgtccccgg
      781 tgggcaactc accaccaaag gtggcgtgct cgaagtgtat atgggccatt acatgcgtga
      841 atggctggca gagcagggga tggtgaaatc gggggaatgc ccgccgccgt acaccgttta
      901 tgcctatgcc aatagtctgc aacgtaccgt tgcgaccgca cagttcttta ttaccggcgc
      961 attcccgggg tgtgatattc ctgtgcatca ccaggaaaaa atgggcacta tggacccaac
     1021 ctttaacccg gtgatcaccg atgattccgc cgcattcagt gaacaggcgg tggcggcaat
     1081 ggagaaagag ctcagcaaac tccagcttac cgacagctac cagctactgg aaaaaatcgt
     1141 taactataaa gattcccctg cctgtaaaga gaaacaacag tgttcgctgg tggatggcaa
     1201 aaataccttt agcgccaagt atcaacaaga accaggtgtt tccgggccgc tgaaagtcgg
     1261 caactcgctg gtagatgcgt ttactttgca atattacgaa ggttttccga tggatcaggt
     1321 ggcctgggga gaaatcaaat ctgaccagca gtggaaggtg ttgtcgaagc ttaaaaacgg
     1381 ctaccaggac agcctgttta cctcaccgga agtggcgcgc aatgttgcga aaccgctggt
     1441 cagttatatc gacaaagctc tggtcaccga tcgcaccagc gcaccgaaaa ttacagtgtt
     1501 ggttgggcac gactccaaca ttgcctctct gttaacggcg ctggatttca aaccgtatca
     1561 gttgcatgac cagaacgaac gcacgccgat tggcggcaaa atcgttttcc agcgttggca
     1621 tgacagcaaa gccaatcgcg atttgatgaa aattgaatat gtgtatcaga gtgcggaaca
     1681 gttacgtaat gccgatgcgt taaccctgca ggcacctgct cagcgtgtga cgctggaatt
     1741 aagcggttgc ccgatagatg ccaacggttt ctgcccgatg gataaatttg atagcgtgtt
     1801 gaatgaagcg gtgaaataac agaaaactcc cccgcgagaa gcgggggagt cgctggttaa
     1861 acgtttttac gttcgatggt ctgttcgccc caaaaaagcg aatctttatc ggtcttagca
     1921 aaggctttga ctaacacttc atcgctacct tcttcccaaa tcttttccgc cattttttcg
     1981 tcgtacccgg cgacttcgaa aatggcctcg gctatttccg gcgacgtatt gcgcagagat
     2041 gcccattcac cgacgtgatg agctttcgct tcttgagttg gcatgcgtat cctcctgttg
     2101 aagattagcc gttaagttta actgccagac ctgcgacata ttccccttga taacgagcaa
     2161 tagacagttc ttcctggctt ggctggcgtg agccgtcacc gcctgcgatg gtggttgcgc
     2221 cgtacggcgt accgccgcga acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa
     2281 tggggacaat taccatgccg tgatgcgcaa gggtcgtcca ggttgaagta atagtttgtt
     2341 cctgaccgcc gccagtaccg gtggaactaa agacgctcgc cagttttccg tatagtgcgc
     2401 cggaagccca caggccgccc gtctggtcga ggaaggtacg catttgaccg gacatgttgc
     2461 caaagcgggt aggtgtacca aaaataatgg cgtcgtaatc ggccagttct tgcggggttg
     2521 caatcggtgc agtttgcgtt ttaccgcctg ctttttcaaa taattgcggc ggcatggttt
     2581 ccggtacacg cttaacgaca acttctgcgc catcgacttt gcttgcaccc tcagcgactg
     2641 cgcgtgccat cgtttcaata tgtccgtaca tggaataata aagcaccaga actttagcca
     2701 tttctaacca ctcctcgtgt tatctctatt ccgtagcgat tcgctaccac ttatttaaag
     2761 ataagacgtc cttttcagag tgcaaatttc acaaccactt atttgattta taacaacttt
     2821 cacaagcacg taattttgtc gcaaaatgac acatttttat ctcatcgcgt ttttttaatc
     2881 ataagagcgg cttatggata attatttgag atgatatcta ttctcgctaa gaagctgttg
     2941 caggatatta ccaaacgcgg gtctgcccgc gtcagttcac taagcttagt cccacgtagc
     3001 gaaaatatgg cagccgccat acgccgcgtt aattctatgc aatatgatgt ctatacccag
     3061 acggaggtca gtaatggcaa accatcgagg cggttccggc aattttgcag aagaccgcga
     3121 aagagcatca gaagcaggta aaaaaggtgg acagcacagc gggggtaatt tcaagaatga
     3181 cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag agcagtcacg gcaaaagcga
     3241 caactagccg ggctaatcaa tgacgaatgc atttttttct taggctcgtc aaaaagtcat
     3301 caccgccggt aatcccggcg gttttctgtt tatggctcct gatgaacaac ttctggcggt
     3361 ggaacgtcaa ccaactttct gcttaacaac gcattgagta aaatcgcgcc aaaggttgct
     3421 gtaccaatcc ctcccaacgt aaaaccgccc agcgtgagag caaaatcacc cgcgcccagc
     3481 actaaggtta ctgcgaccat aatcaaatta ccgttctggc ttaaatcgac acggttttgt
     3541 acccatatcc ttgcgcctgc gacggcaatc agcccgaaca caacaattga tgcaccacca
     3601 atcaccgcgg ccggaatggt atggatcagc gcgccaaatt tcggcgaaaa gcccaacagc
     3661 atggcgatga cggcagcagc aacaaacacc agcgtcgagt agacttttgt cacggccatc
     3721 acaccgatat tttcagcata ggtggtgacg ccgctaccgc cgacagagcc ggaaagcatc
     3781 gttgccagac catcaccgac gaatgctcgc cccatatacg ggtccatatt gcgtccggtc
     3841 atcccggcga ccgccttgag atgacctaag ttttccgcca ccagaatcac cgccacgggc
     3901 gcaatcagca tcattgcctg accattaaaa gcaggagtgg aaaaatgtgg caaaccgaac
     3961 caggcagcat ggctgacgag agtaaaatcg acggcttttc ccagccctaa aacgttggtc
     4021 atcacgccat acagcagaca ggcgacaatt aatcctacga gaatcaataa ccgctggatc
     4081 atgcctcggg taaacaccgc caccagccca atacacagca ccgtcattac cgccatccag
     4141 ctatcaaagg ccgaagccga tacacttttc actgcgatag gcgctaagtt caggccaatc
     4201 gccatcacca ccgcacccgt caccaccggc ggcattagcc gttcaatcca gcgtgtaccg
     4261 attttcatca ccaccaggcc aatgacggta taaaccagcc cacaggcgat aatcccgccc
     4321 agcgcgatgc tgatattcgg gttcatgccc tgaccgttaa agcccgtcgc ggcgatcacc
     4381 acgccgacaa aagccgcgct ggagccgaga taactgggga cgcgcccgcc ggtaataaag
     4441 aaaaacagta acgtgccgat ccccgacatt aaaatggaaa gattgggatc cagccccatc
     4501 agaatcggca ttaacaccgt cgcgccaaac atcgccaccg cgtgttgaac gcccattact
     4561 gccgtctgag caaacggcaa ccgttcatcc ggcgcgacca cgccgctctc tgtagaggtc
     4621 gattttaact gccagtgagg aaaaccgaac attgccatca gctgtctcct taaggaggtt
     4681 aacaagcagg gcgcatcagc gcgtgataac tgcgatcgaa ccacaccagc ccgtagggtg
     4741 tggcgtgacg atgaatcgct tcgatggcgc aaaacagaat gtcgtgggtg ccgacgctca
     4801 ccacctggct gatacggcag tcaaacgaaa ccagggcctc ttccagttgc gggcatccgg
     4861 tcacacccgt ctgccagcgg gcggcggcaa agcggtgttc catgggcgtt ttgccgccaa
     4921 aaaggttgga aagcggctcc tgcccggcgc taagtgtatt tacacacagc gttcgatttt
     4981 cattgaatac cggccagacg gacgccccac gattcaggca caccagtaat gtgggcggcg
     5041 tatcggtcac gctgcagacg gcgctggcgg tgaacccggc gcgcccggct ggaccgtccg
     5101 tggtgatgat attgaccgcc gcgcccatac aggacatcgc atcgcgaaag gtttgttgat
     5161 caactatgtt catagtttgc tccttacaac agcccgcagg cttcttcaaa ggacagacgt
     5221 ggcaggcgcg caaaaagttt gctgctatcg ccatagccga tattaatcag cagattgctc
     5281 ttcagcgtgc tgcccgcaaa aaaggcgtcg tcaacgtgtt gacggtcaaa acccgacatc
     5341 gggccggtat ccagtcccag cgcacggcaa gcgacgatca gataggccgc ctgcatggaa
     5401 ctgttgcgaa acgctgtttc ttcggcaagt tgtgggctgg aggtaaacca actgcgggca
     5461 tcactgtggg gaaacagtag tggtaaccgt tcataaaatt cactgtccca ggcgacgata
     5521 gcggtgacgg gcgcggtcag ggttttttgt agattgccgc tggaaagcgc cgggcgcaga
     5581 cgttcttttc cttctgcagt gcggataaac acgatccgtg ccggagaaca gttagctgat
     5641 gtcggccccc atttcatcag ggcataaagc tcccgtaacg tctcatcgct gacgggtgtc
     5701 tcccgccagc cgttgtgagt gcgggcatcg gtgaacaggg tgctaagcgc acctgggcta
     5761 acggcttcgt tcatagcaat tccttacagg gcggcttcac ggtgatgtaa caggctggca
     5821 agcccgttga gtaacagagc attaaacgtt tcgggatcgg tcacgttgca ggcgtgtccg
     5881 ccatagggca tcaccatttt ctggctatcg ggcagggcgg catgaagttc actggaacat
     5941 gctgttggca ccagcagatc atcactggcg cagatgattt gcaccgggca gcggatgcga
     6001 tccgcatggt gactaaagtc agcgcgtttg agggcgttaa gtcgacgcag taaattattt
     6061 ttgccctgaa aatgcgccag tgccagcgcg tcttctgcct caaggcgagg tgcgcgggcc
     6121 gccatccagt cggcgggata gaggaacaac ggctgcgctt ccacccatgc ctgcgcgccg
     6181 ccgctataca gtaatcgttc gcgaacctga aaacagcggc gcgtatgggc gtttattcgt
     6241 agccagccgt taacgctgat cagcacagtt accgacgcgg gataatccag cgccagctgc
     6301 attcccacca gcgcaccgag cgcatggccg accaccgcgt aatgctcaat ccctgcggct
     6361 accagcgcct gatgcagttc cgctgccatc tgggcgatac tgtaatcttc tgccagtgtg
     6421 tcgggattat tgccggtgcc gcgctggtcg taacagacta cctgatactc ctgctccagc
     6481 accgccagtt gctgtaacca gtaactgccg ctaccgccaa gacccgaaat caacaccact
     6541 acgggcgcat cagcataagg gggaggtgag agtgaaagtt tcatcgcggc ctcacttggc
     6601 gatatgcgca attgtggcga tttccaccag cgcgtcaggt tttaccagtc cgcactgaat
     6661 gcagaatcgc gccggtttat cacccggaaa aaactcggcg tagatttcgt taatcgcggc
     6721 gtaatttttc cagtcggtaa taaagatgct gttgaaggtc acatccgcca tcgtgccacc
     6781 cgccgtctcg atcaccttgc ggatagtttc cagaacgtgg cgggtttgcg cctttgggtc
     6841 atcggcaaac agcacgttat tatgttgatc aaaagccagc gtaccggaga catacaccac
     6901 gccatcagcc agcgtgccgg gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat
     6961 aattacggat tttggcatcg ttaaactcct taagcgatac gagcaaagga tgtgggagaa
     7021 agcgcgtcgc agaacgtttc gacgtcgctg acccagccaa aaaaggtttc gatattgaac
     7081 aacgcggctt tctgtgcaaa ttccggcccc gcctggtgtg ttgcgtcttc cagcaccacg
     7141 ccgaaatact ccagaaaaaa gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg
     7201 ataccggtga aaaccagatg gcgtattcca cggctgcgca aaatgctgtc cagcggtgta
     7261 ttgaagaaac cgctgtagcg cggcttcggc agcacaatat cgccaggctg cggcaccagt
     7321 tcatccacca gttgataatc ccaggagcct ttcgccagca atttcccctg cagctgcggc
     7381 tgattacgca tggttttcag ggcgttcgat ttatgaaaat tcggtgagcc ggggccgcca
     7441 gcctcgacat actgttcatc ccagccattt tgaaaccaga tgatcaacat ccctgccgct
     7501 cgcgctgcgg tcacagcggt ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca
     7561 aacccggcga gatctaagta gccgcctggc gtggcatagg cgttttgcat atccaccacg
     7621 atcagcgcac tttgctgcgg atcgaaggta atggcttccg gtcgagcggt taaggttgtc
     7681 atcatgccac ctcctgagtc agcgcaggta gatgggcgcg gcactgcatc agtggttgaa
     7741 tgcgctcgcc gaaggtttcg attcccgaca gaaaatcatc gaaggtcaac agcacgcctt
     7801 cggcaccagg cacgcttgcg acttcatcta acatgcgcgc gacactggcg taagaaccga
     7861 ctaacgtccc catattgatg tttaccgccg aagtgggatc ggccatctga cggacgttgg
     7921 tatcagtacc ggagcgggta tctttctgac tttgttcggt tagccagctt aacgcctctt
     7981 catccgcgcc cgctttgtag tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg
     8041 caatcaccat aaacaacaca taagaaccaa cgtcgcgccc ggtttgctct gcggcctgtt
     8101 tcatccgcgc agcggtcggg gcgaaagccg tgggcgtatt tacgcctttg ccgaaacaga
     8161 agttgaaatc ggcatactgg gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga
     8221 tcactttcat ggggacactc ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat
     8281 cgcctttaaa atcgcttttc cccgtgcccc acaggtcgcg cagcacctga acatattcgg
     8341 tgagatagtc gtaacgacgg gagaaatagt catcgccagg ccagatgccc atctgctcat
     8401 actcgggctt ttgccagcca gtcacgaggt tgacgccaaa acgcccgcca gagatggagt
     8461 cgatggttgc ggccatacgg gcgacgattg ccggaggtaa cgttaaggtg gcagcagtgg
     8521 cgtaaatctg aatgcgcgag gtgacggccg ccagccccgc catcaaggtg aacgactcaa
     8581 ggttgtgatc ccagaactca gttttgccgc caaagccacg cagtttgatc atcgacaggg
     8641 cgaaatcgaa atggtagtgc tccgcttttt gcacaatggc tttattcagt tcaaaggtcg
     8701 gcatgtactg cggcgcgtgg gtcgaaatga gccagccgtt gttgccaata ggtacgaata
     8761 cgccaatttt catcatcaac ctctcttcgt ctcgtaaagt gaaagtcaga cggggcgctg
     8821 catcctgcat atccttttca gccgcgtaat ggcttgtttg caaagcggat gccagttttt
     8881 aaaaagttaa tgttgttaat atgttaacaa tgcgttatct aaaatatctg gtaaaaagtg
     8941 gactaaacgg tcaaaacagt tgcacataaa acatgcatct gtgcgcgatg agagtgcaga
     9001 aggtcgaggc cgggcggggg ttttgctatc ctgttgccaa tctacaagag gggagagcgc
     9061 atgacgcaag gcgcagtgaa aacaacgggt aaacgttcgc gcgcagtaag cgcgaagaaa
     9121 aaagcgattc ttagcgcagc actggacact ttttcacaat tcggttttca cggcacaagg
     9181 ctggagcaga tcgcagagtt ggcgggtgtt tcaaaaacca atctgctgta ttactttccg
     9241 tcaaaagagg cgctgtatat tgccgtgctg cggcagattc tcgatatctg gctggcaccg
     9301 ttaaaagcgt ttcgtgaaga tttcgctccg ctggcggcga taaaagagta catccgtctg
     9361 aagctggaag tctcccgcga ttatccgcag gcttcgcgcc tgttctgtat ggagatgctg
     9421 gcaggcgcgc cgctgttaat ggatgaactg acgggcgatt tgaaggcatt aattgatgag
     9481 aaatcggcgc tgattgccgg ttgggtcaaa agcggcaaac tcgcgccgat tgatccgcag
     9541 catttgattt tcatgatttg ggcttccact caacattacg ccgatttcgc ccctcaggtg
     9601 gaggcggtga caggcgcgac gttgcgcgat gagatatttt tcaatcaaac ggttgaaaac
     9661 gtgcagcgga ttattattga ggggattcga ccacgttaaa gatgccggag gaggttgtaa
     9721 catcctccgg ctacctgttt aacctatagt cattaaactg gcgttaccgc cagcggcagc
     9781 ggtattcaca ctcagcgaac gttcgatata cagccgttcc agaaggatat tgctttcgcc
     9841 acgggcaaaa ccctgcaccg aaacaattgc gccatcccgc gcagcaacgg cttcacacaa
     9901 tgcgcgaagc tgatccgaat caccgtggaa gatcaccgca tcaaagggtt gagcggttat
     9961 attttccgct ttcgccagtt gaatacgttc gctgactgcc gatggcaatg cctttactag
    10021 ctgacgatgc agcgcgtcat ccggccacag tacctggctg cccaccgcca gcacggcggc
    10081 gagctgagtc agcgcatcct gctcatcatc ggcaatacac aacacgcgct cacgcggcag
    10141 cagcgtccag gtgttgcgtt cacccgtcgg ccccggcagc aatcgttgtg ttcctgcctg
    10201 cgccagctcg ccatattgcg tacataacgc ctgcaattct ggacgatttg ctgcccattc
    10261 ccgcagtgca tttagcggct gagtcaatgc ggctttcaac tgtgcatcga ccggatactc
    10321 tgcatcctga cgcgcgagcg tcactgccag cgcactttcc gggcgattcg ccagcagacg
    10381 gtagagatag agcggaccgc ctgctttcgg cccggtaccg gacaaccctt cgccgccgaa
    10441 tggctgcaca cccaccactg cgcccaccat attacggtta acatacaggt taccaacatg
    10501 ggccgagcca gtgacctggg cgatggtttc atcaatacgc gtatggacac caagcgtcag
    10561 accatagccg gaagcgttaa tctgctcgat cagctctggt agctggttac ggttgtaacg
    10621 caccacatgc agcaccggac caaagacctc tttttgcaat tcggcaaagt catccagttc
    10681 gatcagcgtc ggggcgacaa aggtgccgct ttgccattca cgggcatctt cgctgttttc
    10741 ccgcaccgcc tggaacaccg gacggccttt gctacgcatg gtctgaatat ggcgctcaat
    10801 attggctttc gcttcgctat caatcaccgg accgatatcg gtggtcaggc gacccggatt
    10861 acccatccgg cattcggcca ttgcgccgcg cagcattttc aacgtgtggt cggcaatctc
    10921 atcttgcagg cacagcacgc gcagcgccga acaacgctga cccgcactgt cgaacgccga
    10981 ggccagtaca tccacgacga cctgttcggt cagtgctgaa gaatcgacaa tcatcgcgtt
    11041 catgccgccg gtttcagcga tgagcggaat agggcgaccc tgagcgtcca ggcggctggc
    11101 gatattgcgc tgcagtaacg tagcgacttc ggttgaaccg gtaaacatca ccccgcgtac
    11161 gcgatcatca cccgtcagtt gcgcgcccac ggtttccccc tgacctggca gcaactgcac
    11221 cacgcctggc ggtacacccg cttccagcaa aatggcgatc ccttgcgcgg caatcagcgg
    11281 cgtttgttct gccggttttg ccagcacgct gttacctgcc gccagtgcgg cagcaatctg
    11341 cccggtgaaa atagccagcg ggaagttcca cggactgata cacaccacag gccctaatgg
    11401 acggtgggtt tcgttagcga aatcatcccg cacctgtccg gcatagtagt ggagaaaatc
    11461 gaccgcttcg cgcacttcgg caatggcgtt actgaaggtt tttccggctt cacgcaccag
    11521 aataccaatc agttgctgca tctggctttc cattagcacg gcagcgcgat gcaaaatcgc
    11581 cgcgcgttca accggaggag tggcaaacca gattggcgcg ttattaaccg cactttccag
    11641 cgcctgttct acctcacgcg gcgtggcttc acgcacaaag cccacaatat ctttcggttc
    11701 cgcagggtta ataacgggcg acatctcacc tgccgctacc ggttgttcca gcattggcaa
    11761 ggcctgccat ttttgcagtg cactattgag cagggcagaa gagagcgagg ccagccggtg
    11821 ttcgttagcg agatccagcc ctgccgagtt gtcgcgcccg tgaccgtaaa gatcgcgcgg
    11881 caggggaatt ttcggatgcg gtaatccagt ttgcccttcc tgttgcgcca gtttttctac
    11941 agcagtgacc ggatcggcga ccagttcatc cagtggcaaa gaggtgtcgg caatacggtt
    12001 aacaaacgag gtgttagcac cgttttccag caggcgacgc gccagatacg ccagcagcgt
    12061 ttcatgtgtg ccaaccgggg cataaatacg acacggtcgg ttaagtttac cgtcggcaac
    12121 tttcccggtg acctgctcgt acagtggctc gcccatacca tgcaggcact ggaactcgta
    12181 ctgacccgga tagtagttct gacctgccag ttgataaatc gctgccagcg tatgggcgtt
    12241 gtgcgtcgcg aactgcgggt agattagatt cggcaccgcc agcagttttt tcgcacaggc
    12301 gagataagaa acgtcggtat acaccttgcg ggtataaacc ggataacctt caaggccgtc
    12361 catctgcgca cgcttaattt cactatccca gtacgcgcct ttcaccagac gaatcatcag
    12421 acggcgacgg ctacgggtgg cgagatcaat caggtaatca atcaccaacg ggcagcgttt
    12481 ttgataagcc tgaataacaa aaccgatgcc gttccagcct gccagttccg gctcgaaaca
    12541 gagtttttcc agcagatcga gggagatctc cagacgatcg gcctcttcgg catcgatgtt
    12601 aataccaata tcatactgac gcgccagcag ggtgagtgat ttcagacgcg ggtaaagttc
    12661 ttccattacc cggtcatact gggcgcggct ataacgcgga tgcagcgccg acagtttgat
    12721 tgaaatgccc ggcccttcat agatgccacg accgttagac gctttaccga tggcgtgaat
    12781 cgcctgctga taggaaacca tatacgcctg tgcgtctgcg gcagtcagtg cagcttcgcc
    12841 cagcatatcg taagagtaac ggaaaccttt ctcttccagc ttgcgggcat tggctaacgc
    12901 ttccgcgatg gtttcacccg tgacgaactg ctcgcccatc aggcgcatcg ccatatccac
    12961 acctttgcgg atcagcggtt caccgctttt accgataatg cggttcagcg agcgggagag
    13021 gatggcttcg ttatgggtgg aaaccagttt gccagtaaac agcagccccc aggtggcggc
    13081 attaacaaac agtgacgggc tacgaccaat gtgtgactgc cagttaccgt tgctgatttt
    13141 gtcgcgaatt aacgcgtcgc gggtggcttt gtcgggaata cgcaacaacg cttctgccag
    13201 acacatcagc gccacgcctt cctgcgatga cagcgaaaac tcctgcaata acccctggac
    13261 catacctgcg cgaccactgg catttttttg attacgcagt ttatcggcca gttgatacgc
    13321 cagtttgtgc gcctgtgcag caactggctg cggcaggcgg gcttgttcca gcagcataga
    13381 aaccgcttcg gtttccgggc gacgataggc cgcggtgatc gcggcgcggg aaaccgactg
    13441 gggcaaaatt tgctcggcaa agtcgaggaa tggctggtgt ggttcctctg ccggagtcgg
    13501 tgcttcatcg ctctcattgg cagcgccaga aagcagcgca ggtagctctg gcagagtatc
    13561 gctgttttcc agttgttcga gataagaaaa aatcgcctgc ttaattaacc agtgtggtgt
    13621 gcgatcgata cgtgtcgcgg cagacttaat acgctcacgc gtcgcgtcgt ccagcttaac
    13681 ccccatggtg gtggttccca tgccattact cctgttgttc tgaaaggtgc aacttaacgt
    13741 tatcgtgaaa tatccatgat gttgcaactt tgtgcaacca tgttaaatgt gacctgcgta
    13801 gcaagcttaa aaatgaatga aatgttaata aaagaaatcg atatgacagg gattaaaaaa
    13861 ataaatcaga ctttttctct gcggcagtta acatttttga aaggtgcaac cgcaaaaaat
    13921 gtgagagagt gcaacctgat gaaaaatagt gtcgctgagc actaaaattt aatgtaaatg
    13981 gtgtgttaaa tcgattgtga ataaccagcg cttccggcag gatacggtcg ccctggtaaa
    14041 acataaactc tgttaccccg ttccggtggc agatataacg gcaagtttcg acattgccga
    14101 taataatttt ttggagactt tagatggcta ttagcacacc gatgttggtg acattttgtg
    14161 tctatatctt tggcatgata ttgattgggt ttatcgcctg gcgatcaacg aaaaactttg
    14221 acgactatat tctgggcggt cgtagtcttg ggccattcgt gacggcatta tcggcgggtg
    14281 cgtcggatat gagcggctgg ctgttaatgg ggttgccggg cgcggttttt ctttccggga
    14341 tttccgaaag ctggatcgcc attggcctga cattaggcgc gtggatcaac tggaagctgg
    14401 tggccgggcg gttgcgtgtg cataccgaat acaacaataa cgccttaacc ctgccggatt
    14461 atttcaccgg gcgctttgaa gataaaagcc gcattttgcg cattatctcc gcgctggtta
    14521 ttttgctgtt cttcaccatt tattgcgctt cgggcattgt ggcaggcgcg cgtctgtttg
    14581 aaagtacctt tggcatgagc tacgaaacgg ctctgtgggc gggagctgcg gcgacgatcc
    14641 tttacacctt tattggcggt ttcctcgcgg tgagctggac tgatactgta caggccagcc
    14701 tgatgatttt tgccctgatc ctgacaccgg ttatcgtcat tatcagtgtc ggtggctttg
    14761 gtgactcgct ggaagtgatc aaacaaaaga gcatcgaaaa cgtggatatg ctcaaagggc
    14821 tgaactttgt tgccattatc tcattgatgg gttgggggct gggttacttc ggtcagcctc
    14881 atattctggc gcgttttatg gcggcggatt ctcaccacag cattgtccat gcgcgtcgta
    14941 tcagtatgac ctggatgatc ctctgcctgg caggggcggt ggctgtcggc ttctttggga
    15001 ttgcttactt taatgagcac ccggcggtag caggtgcggt aaaccagaac gctgaacgtg
    15061 tgtttatcga actggcgcaa attctgttta acccgtggat tgccgggatt ctgctgtcgg
    15121 caattctggc ggcggtaatg tcaacgttaa gctgccagct gctggtgtgc tccagtgcga
    15181 ttaccgaaga tttatacaaa gcgtttctgc gtaaacatgc cagccagaaa gagctggtgt
    15241 gggtagggcg tgtgatggtg ctggtggtgg cgctggtggc gattgcgctg gcggcaaacc
    15301 cggaaaaccg cgtgctgggc ttagtgagct acgcgtgggc aggctttggc gcggcgtttg
    15361 gtccggtggt gctgttctcg gtgatgtggt cacgcatgac gcgcaacggt gcgctggcgg
    15421 ggatgatcat cggtgcgctg acggttatcg tctggaagca gttcggctgg ctgggactct
    15481 acgaaattat tccgggcttt atcttcggca gtattgggat tgtagtgttt agtttgctgg
    15541 gtaaagcgcc gtcagcggcg atgcaaaaac gctttgccga ggccgatgcg cactatcatt
    15601 cggctccgcc gtcacggttg caggaaagct aagggactta gcctgcggcg gttttgtttg
    15661 gcttcagcag cgggttgcgc tcccttaatg tgcctcgcca tataaattga atggtgcagg
    15721 gagcgcgcag ggggcggcca atcgccgccg ccccctgctg tcccggcctt cggggaacgc
    15781 ttcagcgatt ttgacgccac caacacccga gctgttatta tgttccgggc aaaaagttag
    15841 atttgataat cgcggatgga cgaaattgct tgatacaccc gcttatcagt tttacatgga
    15901 agctctgatg cattgagtct ggacagtttt gtcggctgga tacggcgttt acgcggcatc
    15961 cggcaagaac acatggttct ttgcaaacaa tcccatcttt ctaccctgga ataatcgttt
    16021 atatcccttg gcattacctc tctttgttta cattccaaca tcattttata aacattccgc
    16081 ttgtattttt ctttaccgta atgataatcg ctatcactgc gatttacttt tctttgcata
    16141 gattgactca gaaaaacgtt taagggtggg tggcatgttt gttccgtttc tcattatgtt
    16201 gcgcgaagga cttgaagccg cgctgattgt cagtttgatt gccagctacc ttaagcgtac
    16261 ccagcgaggc cgatggattg gtgtgatgtg gattggcgtg ttgcttgccg ctgcgttgtg
    16321 cctgggcctg ggtatcttca ttaacgaaac caccggcgaa tttccgcaaa aagaacagga
    16381 actgtttgaa ggtatcgtgg cggtgatcgc cgtggtgatc cttacctgga tggttttctg
    16441 gatgcgcaaa gtgtcgcgca acgtcaaagt gcaactggaa caggcagtcg atagcgcatt
    16501 gcagcgtgga aatcatcatg gctgggcgct ggtgatgatg gtcttttttg ccgttgcaag
    16561 ggaagggctg gagtcggtct ttttcctgct ggcggcattt caacaagatg tcgggatctg
    16621 gccgccgctg ggtgcaatgc tcggtcttgc tactgccgtg gtgctcggct tcctgctcta
    16681 ctggggcggt attcgcctca atcttggtgc attttttaaa tggaccagcc tgtttattct
    16741 cttcgtcgcc gcagggctgg cggctggtgc cattcgcgca tttcatgaag ccggattgtg
    16801 gaaccacttt caggaaatcg ccttcgatat gagcgcggtc ctctcaactc actcgctgtt
    16861 tggcacgctg atggaaggga tttttggcta tcaggaagca ccgagcgtca gcgaagtcgc
    16921 cgtctggttt atttatctca tcccggcgct ggtggcattt gccctgccac cacgcgcagg
    16981 ggcgacagcg tctcgctccg cgtagcaaat acgacgcaaa ctcttgctta gttacaacat
    17041 actttaaagg gatagtctcg tcatgaccat taacttccgc cgtaacgcat tgcagttgag
    17101 cgtggctgcg ctgttctctt ctgcttttat ggctaacgcc gctaatgtgc cgcaggtcaa
    17161 ggtgaccgtg accgataagc agtgcgaacc gatgaccatt acggttaacg ccgggaaaac
    17221 acagttcatt attcagaacc acagccagaa ggcgctggag tgggagatcc tcaaaggcgt
    17281 gatggtggtg gaagagcggg aaaatatcgc ccctggcttt agccagaaaa tgacggcgaa
    17341 tttacagcct ggcgaatacg atatgacctg cggtctgctg actaacccga aagggaagtt
    17401 gatcgtcaaa ggtgaggcaa cggcggatgc ggcgcaaagt gatgcgctgt taagtcttgg
    17461 tggtgcaatt actgcatata aagcgtatgt catggcggaa accacgcagc tggtgaccga
    17521 cacgaaagcc tttactgacg ctattaaagc aggcgatatc gaaaaagcga aagcactgta
    17581 tgcgccgacg cgccagcact atgagcgcat cgaaccgatt gctgaactgt tctccgatct
    17641 ggatggcagc attgacgccc gtgaagatga ttacgagcaa aaagccgccg atccgaaatt
    17701 caccggtttc caccgtctgg aaaaagcatt gtttggcgac aacaccacca aaggcatgga
    17761 tcagtacgct gaccagcttt ataccgatgt ggtcgatttg caaaaacgca tcagtgaact
    17821 ggctttccca ccttcaaaag tggtcggcgg tgcagccgga ctgattgagg aagtggcagc
    17881 cagcaaaatc agcggtgaag aagatcgcta cagccacacc gatctgtggg atttccaggc
    17941 taacgttgaa ggctcgcaga aaattgtcga tctgctgcgt ccacaactgc aaaaagccaa
    18001 cccggaactg ttggcaaaag tcgatgccaa ctttaaaaag gtcgatacca ttctggcgaa
    18061 ataccgtact aaagacggtt ttgaaaccta cgacaaattg accgatgccg accggaatgc
    18121 actgaaagga ccgattactg cgctggcgga agatctggcg caacttcgcg gtgtgctggg
    18181 attggattaa gcattatgca gtatgaagat aaaaacggcg tgaatgaacc gtcacgccga
    18241 cgtttactga aaggcattgg tgcgctggcg ctggcgggaa gttgtccggt cgctcatgca
    18301 caaaaaacgc aaagtgcgcc gggtacgctt tcaccggatg cacgcaatga gaaacagccg
    18361 ttttatggtg agcatcaggc agggatcctg acgccacaac aggccgcaat gatgctggtg
    18421 gcgtttgatg tgcttgccag cgataaaccc gatcttgaac ggttgtttcg cttgttgact
    18481 cagcgttttg cttttctgac tcagggcgga gcggcaccag aaacgccaaa tccgcgcctg
    18541 ccaccactcg attccggcat tcttggcggc tacattgcgc ccgataatct caccatcacg
    18601 ttatcggtgg gtcactcatt gtttgatgag cgctttggcc ttgcgccaca gatgccgaaa
    18661 aagttgcaga agatgacgcg ttttcccaac gactcgctgg atgcggcgtt atgtcatggt
    18721 gatgtgttgc tacaaatttg cgccaacacc caggacacgg tgatccatgc gctgcgcgat
    18781 atcatcaaac atacgccaga tttgctcagc gtgcgctgga agcgggaagg gtttatttcc
    18841 gatcacgcgg cgcgtagtaa aggcaaagag acgccgatta atttgctggg tttcaaagac
    18901 ggcactgcca atcccgatag ccagaatgat aagttgatgc aaaaagtggt gtgggtaacg
    18961 gcagatcagc aggagccagc gtggacaatc ggtggcagct atcaggcagt acgcttgatt
    19021 cagtttcgag tggaattttg ggacagaact ccgctgaaag aacagcagac gatttttggt
    19081 cgtgataagc aaaccggtgc gccgctggga atgcagcatg agcatgatgt tcccgattac
    19141 gccagcgatc cggaagggaa ggtgattgcg ctggacagcc atatccggct ggcgaatccc
    19201 cgcacggcgg agagtgagtc cagcctgatg ctgcgtcgtg gctacagtta ttcactgggc
    19261 gtcaccaact ccgggcaact ggatatgggg ctactgtttg tctgttacca acacgatctg
    19321 gaaaaaggct tcctgactgt acaaaaaagg ctcaatggcg aagcgctgga ggaatacgtt
    19381 aaacctatcg gcggcggtta tttttttgcg ctgccgggag tgaaggacgc gaacgattat
    19441 ctcggaagcg cgttattgcg ggtttaatgt ttatagacgg ataaggcgtt cttgcgcaga
    19501 tgcctgatgc gatgcttgcg cgtcttctta tgcctacaat cggtgcgggt tcggtaggcg
    19561 gataaggcgt tcacgccgca tccggcgttc gtgcgcagat gcctgatgcg aatcctgctg
    19621 aaagcacaca gcttttttca tatctgtcat cactctgtca tctttccagt agaaactaat
    19681 gtcactgaaa tggtgtttta tggttaaata taagtgaata tattgttgca atgaatgcga
    19741 gatctgttgt aattattaag tagcagcgga agttcccggc agtgatagtc agtcactatg
    19801 gagatcgcgg atggtaacgt cctgttctgg acaatgtctt gttaagctac gctccaccac
    19861 ttgcggagat ttctcctccg gtagcaactt cgtcactctg tatttttctc acttcctctc
    19921 tttactcgtg ttaccgacgc cgcaaacggt gtgcgtagcc gtttttcggc attttatcca
    19981 aaagcaaact atggcttaca aggaagccaa ccctctgatg ttcgtgcgca taatcgcgct
    20041 gccaacggcg cgtgtgatga atacaaacaa ctcaaggtgc tctccatggg aagacaaaaa
    20101 gcagtgatca aagctcgtcg tgaggcaaaa cgtgtgctga gacgggattc gcgcagccat
    20161 aagcagcgtg aagaagaata ggtcacctcg cttgtgcaga tgggcggcgt agaagccatt
    20221 ggtatggccc gcgacagtcg cgatacttcg cccatcctcg cgcgaaatga agcgcaattg
    20281 cactatctga aggctattga gagtaagcag ttgatattcg ccacgggcga agccgggtgc
    20341 ggaaaaacct ggatcagcgc agcaaaagcg gcagaggccc tgatacataa ggatgtcgac
    20401 aggattatcg tcacccgtcc agttctgcaa gccgatgaag atcttggctt cttacctgga
    20461 gatatcgcag aaaagtttgc tccctatttt cgcccggtct acgacgtgct ggtccggcgc
    20521 ttaggggctt cctttatgca gtactgcctg cgaccggaaa tagggaaggt ggaaattgcg
    20581 ccgttcgcct atatgcgtgg acgtaccttt gaaaatgcag tcgtcattct tgacgaggcg
    20641 cagaatgtga ctgccgcgca aatgaaaatg tttttaaccc gcctcgggga gaacgtgacg
    20701 gttatcgtca acggtgatat cacgcaatgc gatttgcctc gcggcgtgtg ctccggatta
    20761 agtgacgcgc tggaacgttt cgaagaagat gaaatggttg ggatcgtccg gttcggtaaa
    20821 gaggactgcg tacgttcggc actttgccaa cgtacgctgc atgcatacag ttaagtgtat
    20881 tatcggtgca gagcccgggc gaaccgggct ttgttttggg tgtttatgcc cggactagcg
    20941 ctttttctga aacaaccatt tttatttgcc cctggctggt gaagtgtacg ctcatcctgt
    21001 ggcttttttg tagttgctga tagagctcat caggtattgc taagctctct gcatattctt
    21061 gcggcgtata ttggtaggct gcatgatgct gctgtttttg aaaacgcagc ttattgtaca
    21121 gcgcccagac aattaacacg acggcattcg ccactgccag caaaaaatag aactgaagtc
    21181 ggcttctggc ctcgctttgc cagtaataac ccgtcagcag atccatggcg aataagaata
    21241 tgaacaacgc aaataatgtc cacaagatgg ttgtggcaac ataatcaacc agtaaacgta
    21301 ctggtgattg tcgggtcgta ataattaaat tgttcatatt aacctctcag aatcccgcga
    21361 tcgggactta cccaacgggc gcgttgcttt ttaggcatca acatgactcg tgtaaatgat
    21421 accaatgtcg ttgccaggct cagcatccag aaaataaccg ggaaccaaat aatccagaaa
    21481 agcgatgaag tcagattatg ctcatagcga ttctcgatca tcaggctgac aataaattgc
    21541 agtaaacata acgtacacaa taatattccg gcagtatgtg tcgcagcgat atgtgtcaat
    21601 tcaatattta acggtacacc ggcaagttgg actgcgtaaa taatgaaacc gaccaggcag
    21661 gtgaaggccc atattgtcgt caggcagtat tcaaaaaaca gcggccacat tcgaaagttt
    21721 tctttgcgcc acaaccttgt catatttttg aggaatactt ctgcaccgcc ctgagcccag
    21781 cgcaggcgct gtttccacag cccttttaac gtttcaggca ttaatatcca gcacagcgcc
    21841 cgtggctcgt aaaaaatcgt ccactgattc aactgcagct tccagctaat atcaatatct
    21901 tcggtgatca tatcgtcact ccagtaaccc acttctgcca gggcgctgcg acgaaatgcg
    21961 gcaataacac cggaaacggt aaatacgttt ccatagatac gctgggttcg cttgatcaaa
    22021 ccaataattg aggaatactc gccaacctga attttaccca ccagggtaga acgtgttcga
    22081 atacgaggat taccggttac ggcacccaca cgcgggttgt acaacatcgg ttccacaata
    22141 tatgccgccg catcgcggtc taataacgca tcgccatcaa tgcacaccag atattcactt
    22201 ttcgccgcgg cagctccggt tttaagcgca atggctttcc cctggttttg cgccagatga
    22261 atgacccgca aatggggaat ttgtgcagcc atgcgatcca ggatggcacg ggttttatct
    22321 gttgaaccgt catttacggc aataacttca atgttctcat aacgctgtgc taaagcggcg
    22381 tgtatggttt cctcaacgtt tttctcctca ttaaaacagg gaatgataat ggagatagac
    22441 ggattatctt tcaactgggg agctggtgcg ttttctcccc acggccagtg gcgttcacga
    22501 tagacccaga aatagacgcc gccaacaatc cacataatgg acataaaaaa cggccagaag
    22561 aaaacgaacc tcatcatcag ttcaccagag tgaaagtacg ctacgcatag gggtatgcat
    22621 aacaccagac ataatataaa aaacgatacg atgcgattaa tcatttttcg gataccaggc
    22681 tgttgaaaac tcaggacgaa taaggtctat ttcaggttgg ttatgcagaa aattgtcggg
    22741 ataataacca tagtttttca ctccattcag ttgtaatagg ctcatccagt gagcgagttg
    22801 ttgcgaagaa atagcctgat gctgaccatt tttctgccag ttttgtgcct gtaattctaa
    22861 aatagatttg tctttagcct gagggatgtt tttaatttga ttggtcaatt gtattaacca
    22921 ttggtcagcc gatttttctg cgacaccttc cagataaggc atagccataa tagcggtcca
    22981 gtcatagctt tttaggaaat cagcataatt ctgtgcaaac caggcttcac tttcaggttg
    23041 tattaccgga agtgcaaaaa tatttcgtgc agttttaata tgtggaccgc gaatggcttt
    23101 tacgcgcgca ctaagttcta aagtgaagtc agttaacgca cgacttttaa agcgggccca
    23161 ctgtttaaat tgctccgggt tttgtcgaat ttcgctcaga ctcccgctaa agcctgcttg
    23221 ctgataagcc gtgatagccg gtgcactggc atcttcataa tctgaaagca aagcatcatc
    23281 gtggaacaat atgccatcaa aagcagcatg tccggcaaga tcttcatata acatgccaac
    23341 ttgtgctctg actctgtcat cgaaaggaga gagacggtga tattgttcag gatgaatttg
    23401 tgcttttttc tcccctgttg gtaagtattt tactcgcgtt aatgtgggat ctaaatccca
    23461 gcttaatacc ggcatccacg catagatgtt tacacctgag cgggtacgta attgccaggc
    23521 aacccgacta aaaatatctg ctttcattgg tagcaaacga tttggaaacc agacctcttt
    23581 gaccagccca tcaccatcgg gatcagcaaa tgcctgcaaa tacacggttg atatttgcat
    23641 atctttcacc cgctgaatta gcacatcaat attgcgatcc atttgctgga ggttttcgtc
    23701 ataaacgtaa tcaagatcga tatgcattat ccgttgtggt gatttttcct gtacggtaat
    23761 aatttgctgg gcaaactctt ttaatgaggg attattggcg attaataccc gcggaatgga
    23821 atccaattgc gacgcatttg ccaaacctga ttcaagggtg aagaacatgt cataaccgag
    23881 tttttttaat tcctctatcg ctatgccatt cgcttcgcca taaggccaaa caaaaacgtg
    23941 tggatttacc tcaacctttg tacgcaggta ttccgtcatt tttacagcat ccagacgaat
    24001 tctttcccgg tattctgctg cggtttcata ccgtgcgtgg tcagtaaaat atgcacgatt
    24061 tacatataca ggcaataagc tgccggtggc attagcctga ataccgtagt gagaattcca
    24121 tgtatgagaa gcgagctcaa cgagtcggga acgcgcaact tctcgcactt gttgccacgt
    24181 ggcaaaatat tctcgatcga ccaactcatc gccaaatttt acttgtttat ccgctggcgt
    24241 atcgacccaa ctgccgacgg gggcccatac agcaggccac tggaaggcct gaagaattgg
    24301 gaagacgcgg gtataaaaac tctggtagcc gtcatcaaaa gtcagcacta cagctttttc
    24361 cggtagcggt tttcctcctc gatgtgcttc acgaatttga gcaatactga ccggttgata
    24421 accgttctcg cgcagccagg caaattgttc acgcagtgct gatgtccgca ctgacataaa
    24481 acgctggtcg gcagcttcgt cttcaacgtt atgccatgaa atcgctacaa aaccattatg
    24541 cggccacggt tgctcggcga gtaaagattc gcgatcctgt ggcggtataa atgatgttct
    24601 tgactggcta atgcacgcgg tgagcataat tatactcacc agcatcagga gatatttatt
    24661 tccattacgt aacatattta tccttaaaat ctgaatgtca tatcgaattc aacgtataag
    24721 ttgtgttctc tgtcaccgtc ataaggtcgt ttttcccagc gtagcgttgc gccagcatca
    24781 atcacgtcat tccagctaat gcgttgcccg tagccgagtt gggtgacgac atccgtgcca
    24841 taatgttttt gccaggaggc accaacacct gcgctgaata tttgctccca gctattttca
    24901 tagcttcgcc ataacaaatg gcttgcctca aatgccggaa caatatcgaa cgtttttata
    24961 gggttgtagt atggggtatc gtgttctgta ttttgttcgt aatacagact gggtaggaaa
    25021 tcgacaatca aatatggtga agaccagatg cgttcctgac cctcaagtga gacttcatga
    25081 cgctggttac tgtcggaaaa atcagtgaaa gcccaggaga caccgtactt acgccgctca
    25141 ttttgatacc agcgaacata agcctgagca ctgttgcctg taacaccatt tttcattgcc
    25201 cgtaatggaa cgcggtgaga gaggcgttcc agttgcgaac caatacgcca gttatcatta
    25261 aaatcatacc agccagacag gcgcgcgccg ggtttatgct catgattgaa aacgcgttca
    25321 gcgtactctg cctcgagcca gatattacgt gaccgccact caacacccgc aagccagtcg
    25381 cgaacaatcc cttttccttc gctaaattgt ccatcggcat aaccgaatcc agcaaaaccg
    25441 cgccagttat ccttcagcgg tggtgaataa acgatggtgg ttaagtcgac atcatgttta
    25501 ccactatccg ggccttcggc atcaattcct gttgagccag cgatacgaag ctctgcaaga
    25561 ttatgtacat caaccgcacg ttttaatcgt acaacgccgg gatcttgcgg ttcacgttcg
    25621 acaacatcgt gcgttaagac agctgcctgc tgccattctt gtaacgttaa tgctgtccag
    25681 gcttgttcaa cctccagatt aatattacgt ggctcgatca cttctgcttt ttttaattca
    25741 ttttctgctg cacgaggcca accgcgggct tgtaacacac tcgcgtaatc aatgcgcagt
    25801 ccctgatttc ctggtgcgtt ataagcaagc tctctggctg tcatttcagc ctgaggaaga
    25861 tcattactat attttgctac ggttgagaga aacgaatgcc cctgtaacca ggtatcattc
    25921 gggatgctcg taggcgtgcc cattaaccga aggaaaggcg gcgaagtatt aatggtatgt
    25981 tgggtgacag ttagcgcgcc cggataattt tcactctcca ggtggctgta aaagagatcc
    26041 gcaagttctt catcggataa atccggggca atggtctcct tgtgataaaa gagctcggtc
    26101 attattgact gtgctttttt cggctgatga tctttgagat aagccgatgc aacccaatat
    26161 tgcccccagg gcggaataat ttgccccgtc ttttttaatc gctgatagtg agaaataacg
    26221 tctttataac gatcgcgagt taataacgcg ccaagatgat caacctgaat acgctggtac
    26281 tgggcagtac ggtctgggtt atcgtgccac agaatttcta atgcagcgta ttgggcgagg
    26341 gcgcgatcgg caatggcata acgttcactt tcactgcgcg taggcataaa cgacagtctg
    26401 accagttcgg catgaatatc agcgcgaata tctggcgtta aattggcatc gtcaatcgcg
    26461 gcagcaagtt gattattacg taatgcctgc acgtattctg tgggatattg ttgcgtagat
    26521 gcattttcag gtaatgactc tgtcatcgcc cgtaattcat cctgatgacg ccccgccagt
    26581 ttatagatat aggcttctgc gagtaaattg gctttgtccg gtgctccaga gttaagctgc
    26641 ttaagtttaa ccagcgcagt atcatagtga ccagcatctg ccagggttaa aatttgtccc
    26701 cgttgataat ccttattttg cggctccaga gagagcgcct tttgccacag tgtaagcgag
    26761 ttttgccatt gttgcaggtt acgataagcg acggcgacag ctgcataacc acgcgctggt
    26821 aattgctgat gacggtagcg gttgtaaacg gtaataacct gtttatcttg cccggcccat
    26881 aaggcaatct gtaaccagtc agcaatttgg ttattgctga gtgctgattt tagtgcaaac
    26941 catgacaaag ctggctgagt attacccttg cgagcttcaa taatcaatgc atcataggcg
    27001 ttattaacag cactcttcgc cgcgggactc gctgctaaaa atgcggcagt aagaagtttc
    27061 aaagcccatt tggttttcgg gcaccttttt ctgctacttg aatacatcct gtattactcc
    27121 atgtattgcc aaaatctctc tctgtatcta attacaggta actgaaaaga aagatatttt
    27181 tgcacctcat aatccgttat taaacgcgga agagagacgt gaattgttga tgatgagaag
    27241 aagaaatgat gagcagagtg tccatataaa atccttttct cgcccgaaaa tccattccaa
    27301 tgatgaggat cttcaggaat acggcataaa tcccaatgcc tttttcaaaa taaattagga
    27361 ttaaaataat taaatcagta aattccgatg catgatttcg gatttttcga aagtcctgat
    27421 gaaaggctgc gctacggcac tcacggattt aattgttatt cctaatgtat ctatgaatta
    27481 atgttttata aggattttct ataaccattt ataagtattt tcaagcctgg cttgttgcaa
    27541 acaatgtata aagcacttag gcaataataa ttacattcag caactatcat catcggtatt
    27601 gtttgtgggc ggaatttcat attgacaaca gtacagttct tatatctatt aataatagaa
    27661 agggatctac aacctacaga ttggtgtagc tttatggaaa aagactattt gagaattagt
    27721 agtactgtat tagtgagctt attgtttggg cttgctttgg ttttggtgaa tagttggttt
    27781 aatcagccag gcgttgagga agttgttcca cgatcaacct atctgatggt aatgattgct
    27841 ttatttttta tcgatactgt tgcatttatt tttatgcagt tgtatttcat ttatgaccgt
    27901 aggcaatttt caaactgtat acttagtttg gcttttctga gttgtttgat ttactttgtt
    27961 aaaaccgtca ttatcattca gcaaattatt gaggggcgtt tgacaagcag tgttgtccaa
    28021 aatgacattg caatctatta tttgtttcgt cagatgagtt tgtgcatatt aatatttctg
    28081 gcattggtga ataaagttag tgaaaacaca aaacagcgca atttattttc caaaaaaatg
    28141 actttgtgca taagtttgtt ttttgttttt tgggggccga tagttgctca catactttct
    28201 agtcattatg agagctacaa tttacacatt gccgagttaa ccaatgaaaa tggtcaggtg
    28261 gtttggaaag cctcatatgt taccataatg attttcatgt ggttaacctt gctttcggtt
    28321 aatctatact ttaatggttt acgctatgat atctggaatg gagtgacggt gattgctttt
    28381 tgtgccgtac tgtacaatat ctctctgtta tttatgagta ggtatagcgt gtccacttgg
    28441 tatattagtc gcactattga agtagtcagt aagttaaccg ttatggtaat atttatgtgc
    28501 catatattca gtgcgctacg agtaacaaag aacattgcac atcgcgatcc cttaaccaat
    28561 atatttaaca gaaattattt ttttaatgaa ctgacagttc aatcagcatc agccaaaaaa
    28621 acgccttatt gcgtcatgat tatggatatc gaccacttca aaaaagtcaa cgacacctgg
    28681 gggcatccgg ttggcgatca ggtgataaaa acagtggtga atatcattgg gaaaagcata
    28741 cgaccagatg accttttagc gcgcgtcggc ggcgaagagt ttggcgtctt gctgacggac
    28801 atcgatactg aacgtgcgaa agctttagcg gaaaggattc gggaaaatgt tgagcgttta
    28861 actggcgata atcctgaata cgctatacca caaaaagtga cgattagtat tggcgctgtt
    28921 gtgactcagg aaaatgcgtt aaacccgaat gagatctatc gactggctga taacgcactt
    28981 tacgaggcga aagagaccgg gcgtaataag gtggttgtga gggatgtggt gaatttttgt
    29041 gagtcaccat aaagcggcat tttgatccta cccacgtaat atggacacag gcctaagcga
    29101 ggttcttgtt ttcaaattgt tccggactga ggccgccaca ccaactgtgc cgccgccacc
    29161 gattgtaatc acattcgata taattaaaca ccgttgcccg cattatttcc cggctgataa
    29221 agtgttctcc atggatacat tccactttca gcgaatgaaa gaagctttcc acgcaggcat
    29281 tatcgtagca gcaacctttt gcgctcatac ttccacgcag attatgccgc ttcagttgcg
    29341 cctgataatc tgctgaacag tactggcctc cacggtccgt gtgaacgata acgttccggg
    29401 gcctcttacg ccgccacagc gccatctgca gggcatcgca ggccagttgc gccgtcatgc
    29461 gtggcgacat tgaccagcca ataacggcac gtgaccacag gtcaatgacc actgccagat
    29521 acagtcagcc ttcatctgta cgtaagtacg tgatgtctcc tgcccacttc tggttcgggc
    29581 cactggcgta aaaatcctgc tccaacagat tttctgacac aggcaggccg tgtgcgcggt
    29641 agctgaccgg gctgaacttc cgggaggcct ttgccctcag tccctgacgg cgcaggcttg
    29701 ccgccacggt ttttacgtta aaggggtacc cctgagcacg cagttcatcc gtcaggcgtg
    29761 gggcaccgta acgctgtttt gaccgggtaa aagccgcgag gacaacgctg tcgcagtgtt
    29821 ggcggaactg ctgacgcgtg cttatccttg tccgccgctg acaccacgta taccagccgc
    29881 tgcgggccac ccggagcacg cggcacattg ctttgatgct gaactcagcc tgatgttttt
    29941 caataaagac atacttcatt tcaggcgctt cgcgaagtat gtcgcggcct tttggaggat
    30001 agccagctct tcatcccgtt ctgccagctg gcgtttgaga cgtgcaatct cggtagacat
    30061 ctccagttca cgttcagaag acgtctgctg attttgctgt ttactgcgcc agttgtagag
    30121 ctgtgattca tacaggctga gttcacgggc tgcggcagta acaccgatgc gttcagcaag
    30181 cttcagggct tcactgcgaa attcaggcga atgctgttta cggggttttt tactggttga
    30241 tactgttttt gtcatgtgag tcacctctga ctgagagttt actcacttag ccgcgtgtcc
    30301 actattgctg ggtaagatca ggcggttaaa ggcatcaacc gcgaacaact gcagacgatg
    30361 attacggaat caggcattgc gctggatatt agctgtgata acacgccgcg ccagcaggtt
    30421 attgggggaa cgcaggctgc gttgaatgaa tttgccacgc tgctgatggc cgctggatac
    30481 gaaccggtta aactcggtgt gagcggtgcc tggcacacgc gtttgatgga agacggcgtt
    30541 caggcaatgc gcgattatct tgccggactg gatatcgctt cgccagagca tcaggttctg
    30601 atgaacgtaa cagcaaaaag cgaggtcgct ccgtcaatta tcaaagaaaa tctctcatta
    30661 cacttaactc atacggtgaa atggactgaa tctttcgata catttctgaa tatgccaacc
    30721 ccggtagctt tccttgaaat aagtaataag ccctatttgg ggaatatgtt aaatgatttt
    30781 gctggcgtgg ttcagcaacg agttatgcat tgtcgtaaag catttagtga tgcaaaggta
    30841 ttcaaatgat tcctgattat ttaactttta ttcgctttca ggataaacga aatctgatat
    30901 acatttatgc tattggactt attctgatag gcttttattg gaagaatgca gggtttactt
    30961 ttccatcaga ggatattggt gtagttagtg ggattctggc cctggtgctg tataatttta
    31021 tttttgatct caaggcgtac tgggcttata aatgcgtcac gaagaatatc gatttttcgt
    31081 ggtttaagaa aaagcagaac cacaaaatag aattatttct tacacaacct ctggtggcag
    31141 gatttctgtc gttaatcatg ttgagtgcaa tgagttgggg gctataccag cttctaccct
    31201 cgttatatgc gctgttcctg atttcgttac ttgggccgtt ggtcatcttt ctgctgtttc
    31261 ggatgatccg caccagttat gtcaagcagg tcgctatttc agtagcgaaa aaagtaaaat
    31321 ataaaagtct gactcgctat gtgctgcttt cggtgtgcat ctcaacggtt gttaacctgc
    31381 ttactatcag cccgttgcgt aacagtgatt cttttgtgac agaggggcag tggttaacgt
    31441 ttaaatcgat aattgcattg ctcattcttt gtggcgtagt gttggcgatt aatctgtttt
    31501 ttctgcgctt ctccaagcgg tacgcttttc tgggcaggct ttttttgcag gaaatcgatc
    31561 tgtttttctc cagtgaaaat gcgttgtcga ccttttttgc caagccgctt tggcttcggt
    31621 tattcatatt gctggttatt gaaatgatgt ggattacgct ggtgtcggta ttggcaacgc
    31681 ttgtggaatg gcggatttgg tttgaagcct attttttact ctgctatgta ccgtgcttaa
    31741 tttactattt tttccattgt cgattcctct ggcataacga ttttatgatg gcatgtgaca
    31801 tgtatttccg ttgggggcat tttaataagt gaggaagtta caggaagtga ccagataacg
    31861 catatatgtt ctctactatc ttgcgcattt tgattgttga ctgagtaacc agacagttga
    31921 tgtgcacaat ttcccctcgc cctaacagac gtgggcgggg gcacccggtg tgtgcaatat
    31981 acgaaaaaaa agcccgtact ttcgtacggg ctcttcttta aatatggcgg tgagggggac
    32041 cgcctgaaat aaatctgact taatccaggg gtgttatata atttcattat aaacaatgta
    32101 ttacactttt tgttgtccga tattatctaa tgtattctta gtgaagcgag agactaatgg
    32161 catactggat ggcataccag agtgacggga attagcgtat ggcagtattg acggatacga
    32221 aagcaagaca tatcaaacct gatgacaaac cattgcccca tgggggaatt accggactga
    32281 cccttcatcc ttcttcagta aaggggcggg ggaaatgggt ttttcgttat gtaagtccgg
    32341 tgacacaaaa aagacgtaat gctggattgg gaacttaccc agaggtcagt attgctgaag
    32401 ctgcacgtac tgcccggata atgcgagagc aacttgctgc aggtgatgat cctctggaga
    32461 ttaaaaaggc tgaatctgag aaagtcgcta tcccaacatt tgccgatgca gccaggcgtg
    32521 tacatgcaga actgtctcct ggatgggaaa atccaaagca tgtaaggcag tggttatcga
    32581 cgcttgagaa ttacgcgttt cctcaactgg gagcaaaaac gctggattcg attacggctg
    32641 cggacgtggc agaaacactg cgtccagtct ggttaacctt gtcagaaacg gcaagccggg
    32701 ttaaacagcg cattcatgtt gttatgcagt ggggatgggc gcacggtttt tgtgtagcaa
    32761 atcctgttga tgtggttgac catttgcttc ctcagcagac aagaggacgt gatgaacacc
    32821 aacccgcaat gccctggagg cagttaccgc tttttgtggc gaccagtgtg tataccgatg
    32881 aaccttataa tgttacccgc gcactgttat taatggtgat acttacagca actcgctcgg
    32941 gcgaagccag gggaatgcgc tgggctgaaa ttgattttca taagcgggta tggactatac
    33001 ctgcagaaag aatgaaagcc aggatacagc atcgtgttcc tttatcccgg caggctattt
    33061 acattctgga aaatatacgt ggcctgcatg atgaactggt gttcccttca cccagaaagc
    33121 agcagatcct ttccgatatg gtgttgacaa gttttctgcg taaaaagaaa gccgtcagtg
    33181 acattccggg gcgagttgcc acggcacatg gttttcgctc aacattcagg gactggtgta
    33241 gcgaacaggg gtattcgcgg gatctggcgg aaagggcgct cgctcatacg ctgaaaaata
    33301 aggttgaggc ggcatatcat cgtactgatc tactggagca gcgtgtaccg atgatgcagg
    33361 catgggcgga ttatgtgatg tctcaaattg tgaataaata atagtgaaaa tgtatattgt
    33421 acgatcatga tttgatatgt gatcgtagca gtagtaatta agaaaccgct tttatgtcaa
    33481 atgtattgag ctgataatta tcagatcgtc aggaaagcgt attttgatta acaggggatt
    33541 acttaacaat ccccttttta taaaaaataa tttgcctgtt taactcttca ctcctgctga
    33601 gactgaatta ttcctagtgc gtcataaatc agtcttctgc gtagtttatt gtttttatct
    33661 gcaccatata acataaatat attttcaact ttttcttcag gtgttctgag ataatggata
    33721 agaaaggtta tcattccaac acaatggaat aaagtgaatc catcaaacag gcttatcagg
    33781 tttccttttt tttcccgtgt tgtaagtatt ttttcgcggg taagaatatt tgccagttca
    33841 tggggggctg atatgtttct gctgtctata atgtcattta ttttaccaag ggctatatca
    33901 acttcctgta ataggttatt tatcatagct gagtgaatgt tattggtttc atgttcctga
    33961 cagtttatga acttataaat cattgagtta tacagaaat
//
LOCUS       DAZGCS010000050        33903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGCS010000050 DAZGCS010000000
VERSION     DAZGCS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.050"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..329
                     /gene="ydjO"
                     /locus_tag="ACHK01_002460"
     CDS             <1..329
                     /gene="ydjO"
                     /locus_tag="ACHK01_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO8097401.1"
                     /translation="KNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSS
                     LRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQ
                     NGPLPA"
     gene            complement(333..1724)
                     /gene="tcyP"
                     /locus_tag="ACHK01_002461"
     CDS             complement(333..1724)
                     /gene="tcyP"
                     /locus_tag="ACHK01_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO8097402.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1857..2447)
                     /gene="ydjM"
                     /locus_tag="ACHK01_002462"
     CDS             complement(1857..2447)
                     /gene="ydjM"
                     /locus_tag="ACHK01_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8097403.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2610..3278)
                     /gene="hxpB"
                     /locus_tag="ACHK01_002463"
     CDS             complement(2610..3278)
                     /gene="hxpB"
                     /locus_tag="ACHK01_002463"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO8097404.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3425..3961
                     /gene="yniB"
                     /locus_tag="ACHK01_002464"
     CDS             3425..3961
                     /gene="yniB"
                     /locus_tag="ACHK01_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO8097405.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4002..4862)
                     /gene="yniA"
                     /locus_tag="ACHK01_002465"
     CDS             complement(4002..4862)
                     /gene="yniA"
                     /locus_tag="ACHK01_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO8097406.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4968..5258)
                     /gene="ghoS"
                     /locus_tag="ACHK01_002466"
     CDS             complement(4968..5258)
                     /gene="ghoS"
                     /locus_tag="ACHK01_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8097407.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5359..6288)
                     /gene="pfkB"
                     /locus_tag="ACHK01_002467"
     CDS             complement(5359..6288)
                     /gene="pfkB"
                     /locus_tag="ACHK01_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO8097408.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6575..7333
                     /gene="ydiY"
                     /locus_tag="ACHK01_002468"
     CDS             6575..7333
                     /gene="ydiY"
                     /locus_tag="ACHK01_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO8097409.1"
                     /translation="MKLLKTVPAIVMLARGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7386..7493)
                     /gene="yniD"
                     /locus_tag="ACHK01_002469"
     CDS             complement(7386..7493)
                     /gene="yniD"
                     /locus_tag="ACHK01_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO8097410.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7669..9567)
                     /gene="espL1"
                     /locus_tag="ACHK01_002470"
                     /pseudo
     CDS             complement(7669..9567)
                     /gene="espL1"
                     /locus_tag="ACHK01_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10091..12019
                     /gene="thrS"
                     /locus_tag="ACHK01_002471"
     CDS             10091..12019
                     /gene="thrS"
                     /locus_tag="ACHK01_002471"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO8097411.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEMFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12023..12565
                     /gene="infC"
                     /locus_tag="ACHK01_002472"
     CDS             12023..12565
                     /gene="infC"
                     /locus_tag="ACHK01_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO8097412.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12662..12859
                     /gene="rpmI"
                     /locus_tag="ACHK01_002473"
     CDS             12662..12859
                     /gene="rpmI"
                     /locus_tag="ACHK01_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO8097413.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12912..13268
                     /gene="rplT"
                     /locus_tag="ACHK01_002474"
     CDS             12912..13268
                     /gene="rplT"
                     /locus_tag="ACHK01_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO8097414.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13385..13509
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13391..13435
                     /gene="pheM"
                     /locus_tag="ACHK01_002475"
     CDS             13391..13435
                     /gene="pheM"
                     /locus_tag="ACHK01_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO8097415.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13719..14702
                     /gene="pheS"
                     /locus_tag="ACHK01_002476"
     CDS             13719..14702
                     /gene="pheS"
                     /locus_tag="ACHK01_002476"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO8097416.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14717..17104
                     /gene="pheT"
                     /locus_tag="ACHK01_002477"
     CDS             14717..17104
                     /gene="pheT"
                     /locus_tag="ACHK01_002477"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO8097417.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHSLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGKRIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17109..17408
                     /gene="ihfA"
                     /locus_tag="ACHK01_002478"
     CDS             17109..17408
                     /gene="ihfA"
                     /locus_tag="ACHK01_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO8097418.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17509..18489
                     /gene="btuC"
                     /locus_tag="ACHK01_002479"
     CDS             17509..18489
                     /gene="btuC"
                     /locus_tag="ACHK01_002479"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO8097419.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            18552..19103
                     /gene="btuE"
                     /locus_tag="ACHK01_002480"
     CDS             18552..19103
                     /gene="btuE"
                     /locus_tag="ACHK01_002480"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO8097420.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19103..19852
                     /gene="btuD"
                     /locus_tag="ACHK01_002481"
     CDS             19103..19852
                     /gene="btuD"
                     /locus_tag="ACHK01_002481"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO8097421.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19930..20394
                     /gene="nlpC"
                     /locus_tag="ACHK01_002482"
     CDS             19930..20394
                     /gene="nlpC"
                     /locus_tag="ACHK01_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8097422.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            20641..21354
                     /gene="ydiV"
                     /locus_tag="ACHK01_002483"
     CDS             20641..21354
                     /gene="ydiV"
                     /locus_tag="ACHK01_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO8097423.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLYKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21417..22853
                     /gene="selO"
                     /locus_tag="ACHK01_002484"
     CDS             21417..22853
                     /gene="selO"
                     /locus_tag="ACHK01_002484"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO8097424.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(22857..23048)
                     /gene="hemP"
                     /locus_tag="ACHK01_002485"
     CDS             complement(22857..23048)
                     /gene="hemP"
                     /locus_tag="ACHK01_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO8097425.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23180..24226)
                     /gene="aroH"
                     /locus_tag="ACHK01_002486"
     CDS             complement(23180..24226)
                     /gene="aroH"
                     /locus_tag="ACHK01_002486"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO8097426.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24383..25216)
                     /gene="ppsR"
                     /locus_tag="ACHK01_002487"
     CDS             complement(24383..25216)
                     /gene="ppsR"
                     /locus_tag="ACHK01_002487"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO8097427.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            25549..27927
                     /gene="ppsA"
                     /locus_tag="ACHK01_002488"
     CDS             25549..27927
                     /gene="ppsA"
                     /locus_tag="ACHK01_002488"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO8097428.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(27984..29630)
                     /gene="fadK"
                     /locus_tag="ACHK01_002489"
     CDS             complement(27984..29630)
                     /gene="fadK"
                     /locus_tag="ACHK01_002489"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO8097429.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLITAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCVRLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(29687..29980)
                     /gene="ydiT"
                     /locus_tag="ACHK01_002490"
     CDS             complement(29687..29980)
                     /gene="ydiT"
                     /locus_tag="ACHK01_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8097430.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(29977..31266)
                     /gene="ydiS"
                     /locus_tag="ACHK01_002491"
     CDS             complement(29977..31266)
                     /gene="ydiS"
                     /locus_tag="ACHK01_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8097431.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31322..32260)
                     /gene="ydiR"
                     /locus_tag="ACHK01_002492"
     CDS             complement(31322..32260)
                     /gene="ydiR"
                     /locus_tag="ACHK01_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO8097432.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32280..33044)
                     /gene="ydiQ"
                     /locus_tag="ACHK01_002493"
     CDS             complement(32280..33044)
                     /gene="ydiQ"
                     /locus_tag="ACHK01_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO8097433.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASGIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            33360..33903
                     /locus_tag="ACHK01_002494"
                     /pseudo
     CDS             33360..33903
                     /locus_tag="ACHK01_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
BASE COUNT         8563 a         8104 c         8652 g         8584 t
ORIGIN      
        1 aaaaaaatga gagtctccta aagtttcctt cgataatatc aatgcttaat atcaatcttc
       61 tttttaatcc actaaatctt ccgggaatgg gaagtggtgt gcttgaagat attatgagta
      121 ttccagatag ttcacttaga aaacgtctcg ggtatgaggt tttaagcttc tcactacagg
      181 cacattcatt gagccaggaa tgtattgata agttggacat ttttttcgct gacgatctgt
      241 ttaaatatga gtcagtatgc atcgccgcga tggaacattt aaaaagtaaa gcaacggccc
      301 ctatacaaaa cggaccgttg ccagcataag aattaacggt gtgccagttc ggcgtcgtct
      361 tcgctatcca gaatggcttt atcggtttgc ttcagccact ggctggtcag cgtgccagct
      421 gtcatcgagc cactaacgtt tagcgccgta cggcccatgt cgataagcgg ttcaacggag
      481 attaacagcg ccaccagggt tactggcagg cccatcgcag gcagcacaat cagtgcggcg
      541 aaagttgcac caccaccgac accggcaacg cctgcggaac taacggtaac aataccgacc
      601 agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa ccgtaggcgc aaccatcacc
      661 gccagcattg ccggatacaa accggcgcag ccgttctgac caatggttgc accgaaagag
      721 gcggcgaaac tggcgatgga ttcaggaacg cccagacgac gcgtttgtgc ttccacattc
      781 agtgggatag acgcagcact ggaacggctg gtaaaggcaa acgtcagcac aggccatacc
      841 ttacggaagt acttcagcgg actcacgcca ttaatgccca gcagaatgcc atgcactgca
      901 aacataatca gcagaccgag gtaggacgcg acaacgaaac ttcccagttt gatgatgtct
      961 tgcaagttag aacctgcaac cactttggtc attagagcca gaacgccgta aggggtcaat
     1021 tgcatgacaa ggcgaaccag tttcatcacc cagctttgta gggtatcgat agcggctaag
     1081 acgcgttcac ctttcggcgc atcatccttc agcagtttca atgcagctac gccgaggaat
     1141 gcggcaaaaa ttaccacgct gataattgac gtcggattgg ctccggtaag atcggcaaac
     1201 gggtttttcg ggataaagga caagaccagc tgcggaacgc tcagatcaga gactttacca
     1261 acatagttac tttcaatggc gttcagacgt gcagtttctg caccaccctg aaccagacct
     1321 tcagccgtca aaccaaacag gttggtgacc agcacaccga ccagcgccgc aatcagcgtg
     1381 gtaaacaaaa gcgtaccgat ggtcagaaaa ctgattttgc ctaactgaga tgcgttatgc
     1441 agacgggcaa ccgcgctcag aatagaggcg aagactaacg gcataacgat catttgcagc
     1501 agttgaacat agccgttacc aacgatgtta aaccactgta cagaatcttt aagtacctgg
     1561 ctgtcagaac cataaatggt atgcagggca aggccaaaaa ccacacccat taccagaccc
     1621 accagcactt ttttcgccag actccactgt ttatggcggg tctgagccag cgcaaacagc
     1681 agtacaacga acaccacgat gttcgcaatt aatggaaagt tcatccccgt tctcctgatc
     1741 ttattatttc tatcactgcg tagcagtcat agtgttggcg caagattaac agaactgtga
     1801 gctggctctt atattcaaat ggaattgctt attccagaaa ggtgattttt gtctttttag
     1861 tattcaacct gatgcttaat aagccgatga aactggatct gcaaggtatt gatcatttgc
     1921 gatgaccaac gaacagcgct gttctcgggt aatgaatggg gcatccatac cgaccagaca
     1981 aataatgcca tgcagataaa acgttccagt tggttgccct tttggggaac caggataggc
     2041 aagcggaaac gccagcggca tggccagagc aggggaacac cggcgggtgt cagcatatcg
     2101 gcaagtatgt ggctcaaata acccagcacc attccttgta gcgcatcagc cggaatgaac
     2161 cagccttccg gaaccttaag gtaaaaggtt gccagcagcg caaataccgc cagcagactg
     2221 tgggtaaaac cacgatgccc aaaagcgcgg gcgatcggtt ttgatatcca ttttaatcgc
     2281 tgcccaagaa acgactttgg gtgatcgatg tccggtaaca aacacgtcag gattgcggaa
     2341 gggacaatat gccaccagtc accctgtgcc agcacgggcg tcagctcggc atttttggca
     2401 aataccgcac aagcaataga aaagagaagg tgaccttccg ccgtcatgat aaaactcact
     2461 aaactgtcga tacgtacagt ataggatttt tatacagtgc acggaagtgg tgacggtgta
     2521 actctttctc ttaaattgac gattaatccg tcagttgaat gcaaattcag ataaagaacc
     2581 aggggcagcg agatcactgc ccctggtcat taaccgagaa ggtcttttgc ggtgagttct
     2641 gtcagcgatg agagtttgac gtttgctaat acaaaacgtg gatcattttg cgcttctggc
     2701 gcaggaacga caatggaacg catgcgggct gctttagagg cgatcatgcc atttactgaa
     2761 tcttccagcg ctacgcaggt cagtgggtca acgcccagtt ttgctgcgca gtcgagatat
     2821 acttgcggat gcggcttgct gtaaggcagt ttttcagccg aggcgagggc atcgaaactg
     2881 tcgcgtaagt caaacatggt caacactttt tccagcatat gtagtggtga cgcggaggcc
     2941 agtcccacca ataaaccttg ttctttgcat aacgcaacgg cttcgcgcac gcctggtaat
     3001 aatggacgtg tctcttcaac cagtgaaatg gcacgggcaa taacccgttc tactacttcc
     3061 tgacggcttg gcccattcca cggttgccgg gcgtaccaaa gatcgaccac catatcgatg
     3121 cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga tatccacccc caggcttgcc
     3181 atcacatcca gttcggctcg atcccataaa ggttctgagt cgataagtaa tccatccata
     3241 tcaaaaattg cagcaagaat ctgacgcggg gttgacatta caacctctcc tttagagcat
     3301 tatgtgtaag gtattaagcc gtgcttatga ttaagcgcct atcatatcgc ctttaaagaa
     3361 tgggcgaaaa cagatatcta caggtaaact aagtgaaacg acgcgtctta atcaaggaga
     3421 actcatgacg tatcaacaag ctggacgcat tgctgttttg aaaaggattt tgggttgggt
     3481 gatttttata cctgcattga tctctacatt gatttctttg ctgaaattta tgaatacccg
     3541 gcaggaaaac caggaaggca ttaatgcggt catgctcgac tttactcatg tcatgatcga
     3601 tatgatgcag gcgaatacgc cttttttgaa tctgttctgg tataactccc cgacccctaa
     3661 ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc atcctgattt ttgtcggact
     3721 ggcgttgcag gattccggtg cccggatgag tcgccaggcg cgcttcttgc gggaaggtgt
     3781 tgaagatcaa ctcattctgg aaaaagccaa aggggaagag gggctaacgc gtgaacaaat
     3841 tgagtcccgt attgttgttc cgcaccatac tattttcctg cagtttttct cactatatat
     3901 cctgccggtt atttgtattg ctgcaggtta tgtgttcttt tctctgcttg ggtttattta
     3961 atcaaaaggc cgcacggagc ggccattatc ctcaacccat atcatgctgc taataatcta
     4021 tccaatgact gctgagcaat aaccaaatgc tgaccgccaa ataatcttgc acgatttaac
     4081 agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat cggcaggtag cggggatact
     4141 gactgatagc cgtcatagat ttgtggcggc tgttcagtat gcagcggtaa catcgccagg
     4201 tcgcactctc ggtcacccca gtagcaggca gggtcgaaaa tgtacgggcc atccggaccc
     4261 agcgcacagt tgccggacca taaatcgccg tgcaacagag aaggctgcgg ttgatgcgag
     4321 gccagacgct gctgaatatg ctcgacgatg gcgtcgatat tgccgaaagc gatccctttc
     4381 tctgctgcca gttccaactg ccagccaatc cgttgttcag caaaaaacgt tgaccagcga
     4441 cgttgccagg tgttgggctg tggagttgtg gagagcgagt tatcaaaatc gaggccaaat
     4501 tgtggttggt cactccattg atgtaaacgc gcaatttgct gaccaagaat aaatgcgcta
     4561 tgcgcatcca gcggacgagg tgggagataa tccatcacca gaaaactgta gtcacggtca
     4621 gcgcctactg cccaaacctt aggcacggtg acggttttac tacgcgacag taactccagt
     4681 tggtcggctt cggcggtaaa accgggaagc agttcccttt catcacattt gacgaaaaag
     4741 tcatgtcctg catagcgcaa atgccatgcg gcatggactt ctccgccagg cagttcatta
     4801 cgcagttcga tttcgccttc acctaactgc tcgctcaaaa gacgactgat tgcctgccac
     4861 atgatgtctc tcccatgttg tctgccagat cataaggtta gcgcaagaat gcggtgtaaa
     4921 aacacgagct aacgcacacc acagactgat tattgcccaa aaaccattta ttttctgttg
     4981 ctattccatt cctcccaggt cgtgatggtg atttcaggat ctttgccggt tgcactttgg
     5041 gtaagccccg aaaccagttc tctaatttct tcttcacttt gggtactgat aagtccaaaa
     5101 gtgttagtcc ccagctcatg gatatttccc tcatcatccg tcatggtgag caaaaaaccg
     5161 tcgcgagtca ggtaattatt caactcgtta atttcagtca atgtatcctc atgcaacatg
     5221 acggttatga cataacggac aagatcgcca ctggccatag ttcacccctt tgttatcaca
     5281 tgacttttga gcatagtcgg agaaacgcgt tgccgacagg ttggtggtga ttcccccaat
     5341 gctgggggaa tgtttttgtt agcgggaaag gtaagcgtaa attttttgcg tatcgtcatg
     5401 ggagcacaga cgtgttccct gattgagtgt ggctgcactc cccgcagcta cgccaaaacg
     5461 gaccatctct tcaagagagg cattttctgc cagtttcagt gtcatcgcgc cgaccatgct
     5521 gtcaccagcg ccaacggtac tctggctttt caccggtggt ggcaccacct gaatgcagtt
     5581 ttcactatca acacccagcg ctccttgtgg accaagggaa acgacaaccc gtttggcctt
     5641 gccgctatta acgatttcct gcgcggcttt gcggacatcg tctggctggg tgagttcgcg
     5701 attcaccagc gcactgagtt ctttttggtt aggcttaacc aactcgatgt taccaatcgc
     5761 cagtgctgca cttaacgctt cgccagaact gtcgatgatg cagcggatcc cttggttttg
     5821 cgcagcggaa atcagttggg ttaatttttc cagcttcaca cctggcggca ggcttccgct
     5881 tatgaccagg atggccccgg attcaatttc cagaacttgc tcttcaagct ggcgaaactc
     5941 atcttcattt aatgccgcgc ctggcataac aaaacgatac tgctcaccgc ttgcttccac
     6001 atgtacgtgt aaattctgcc gggtccagtc tttggcttct acagtagcga cggggacatt
     6061 ttcatccgcc aacagagaaa ccaggtgttc gccggtcgcg ccacccgccg ggaagatcgc
     6121 tgtggcactg cctccaagat gggcaatggc gcgggcgacg ttgatgccgc cgcccccggg
     6181 ttcgaacacc ggtgcggtac agcgcagttt tccttcggga taaatttgcg gggtaattgt
     6241 tgcgctatcg agagagggcg caagtgtcaa cgtatagata cgtaccatca tttcctccta
     6301 taggctgatt tcagtctggc accgaatcag ccgaaagtga agaaattaac aatatgattt
     6361 attagagctt tatttaaaat tttgcagata aatatatata aataaaaatc tgttttcgcc
     6421 acatgcttat tcaaaaaatg aaataagaat acattgctaa gttattcgcg cctttttata
     6481 attcgttacc tttccaggga cgctaatctt tgatccttaa gaaagtttcc gggaccgcga
     6541 tggtctcttt ttttgttgta cggactcttg ataaatgaag cttttgaaga cagttcctgc
     6601 catcgttatg ttggcgaggg gcatgtttgc gtcactgaat gcagctgccg atgattccgt
     6661 ttttactgtc atggacgatc ctgccagcgc taaaaaacct ttcgaaggta atctgaacgc
     6721 aggatatctt gcacaatcag gcaatacgaa aagctcctca cttacggctg acaccaccat
     6781 gacctggtat ggacaaacca ctgcctggtc gctgtggggg aacgccagta atacttcttc
     6841 taatgatgag cgttcttcag aaaaatatgc ggcgggcggg cgtagccgtt ttaatttaac
     6901 tgattatgac tatttatttg gtcaggcaag ctggctgaca gaccgttata acggctatcg
     6961 agagcgcgat gtgttgaccg cgggttatgg tcgtcagttt ttgaatggcc cggtacacag
     7021 tttccgcttt gaattcggtc caggtgtgcg ttacgacaaa tataccgaca atgccagtga
     7081 aactcagccg ctgggctatg cctccggtgc ttatgcatgg cagttgactg acaacgctaa
     7141 atttacgcag ggtgtttccg tctttggcgc ggaagacaca acgctgaact ccgaaagcgc
     7201 attaaatgtt gctattaatg aacattttgg actgaaagtg gcttacaacg tcacatggaa
     7261 ctctgaacct ccagagtctg caccggaaca taccgatcgt cggacaacct tatcgctggg
     7321 ctacagcatg taaaattatc tgccggaccg tgttatgcgg tctggctgtc ttcgtttgtc
     7381 actgctcagc cccaaatcat tgctgcccaa cgtagcaaca acatagcgcc acagattgcc
     7441 agtagcacca ccaccatctt ccagtgtttt ttatcaaagc gtttagtcgg cataccagtg
     7501 tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa agggacaggc atcaagggat
     7561 tttttgttat tacagccgtc gggtggcgtt gtacacctga tcactgtttc tgcgttttat
     7621 gaaaaaaata aaattatgta tcgatataca aatatcataa atagctggtt ataaatacgt
     7681 ttttagttca tcagcactaa aactcaggct ttggctgatt gcattaatag cagcataata
     7741 tgtttctaca gttttataat gattatgatg aatggctata tgaacagctg acatgttttc
     7801 atgattttta gcggccaaca gtgtgaataa ttgacgttga ctaaaagaat atttttttgc
     7861 aaaagtactc agtgttgata tataagataa cacgacgtct ttattaccct tgctcatggc
     7921 gatgtataaa gcaggggttc catgtgctgt cgcgcctttt aataagtcca tgatgttgat
     7981 tttgctgagt ttatatttaa cggcgatccc ataaacttta gagaggaacc gtgtggcaca
     8041 caaagggtga ttattttcca ttgcagcaaa taaccctggt tcaccatcac gtgttttcat
     8101 ggctaaaata tccatcacca tttttttgtt taaaagttct tgtgctattg gagtggttaa
     8161 tgcattcaga aagatatcga ttatttcatc gaatccatac aacatggaga tatagagtcc
     8221 aggtgtttcg ttagcacttt tagtttgcag taatttaacc agattatctt catgaatgat
     8281 atgattgtat accagtgact ggatttcttg cataaatatt tttacgttgt cagtttgtcc
     8341 gttcatcata gctagaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttc
     8401 aatgatttct ttttcaggga gtatgggcaa ctgtttgaat agtgtaataa gctattgagt
     8461 ctggcctgtg gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg
     8521 tgtcgtgata cccgctaaaa tagcaactgg aactccctca ggtaatagag gaagaatagc
     8581 aattgcacag ttgcgaacat acctatcagc ccaggtctga gcatcgggag ctgaactgat
     8641 gaacttatca agcgagagaa agcgccgtgc tgtcctgtgg gtgcctctga cggcaacgtt
     8701 ggtatcgttc gggtcgtaaa cagacacaac ataatttaat tgtccataca ctgactgctt
     8761 tattcgtagc cttactgaca aagtatgatt gtctatatat aaaatcgcgg acatatgact
     8821 gacattgttt gccatcattc gacaaaagaa aatatctaaa gcgaagccaa aatccttact
     8881 cgcgataacg cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcatcccg
     8941 gactaagggg atatgctgct gtatggattc aacggaggag taatgtttaa tcaggttgac
     9001 tttgcctttt ccatttctca atgaatcaag ggcgtattgc aatgacagat ggcgacaaaa
     9061 aatagcgtca gaaggagatt gcaaaaaaca tgcactacca ttgagattta ctgttgggtg
     9121 tatagttttt tctctatgga tttttttaga cagtttcata caatggttac gatcttcagc
     9181 agttaactgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
     9241 cgtggtttcc agtaataagg aaatgaaatc ttttttatag tgtaatgtaa tgagtatatt
     9301 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
     9361 ttgtgatatt tttcgacagt ttgcggtaaa ttcctgaata acacagtatg acagcatcga
     9421 acttaagtca gaggtatcaa gtagggtgat gagctggcgc gcaaatttat taataggctc
     9481 tgatgaaggt gttggtatgt cgctatcgat gtgcaatcta tgaggagact tataatgagt
     9541 tctgataatg atatcgtttt gactcataat ctattcgcct tatatatatc tggaggtttt
     9601 gaggatgatg attaattatt atcgccagaa cgaaatgtgc acgaaaaatt gttataagaa
     9661 ttgtgtcact tttatcattg tgatatgacc acttcgtcat taatctcatg agcgtttaaa
     9721 atactaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc
     9781 gctcgcagta aacctttcag acgcaccgtg atgttatcag tggttcttta agcgttttgc
     9841 tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc
     9901 tccctttttt tagtgttaac atcgctcaac cgggtggaag gtgagaggtt aatgtctttc
     9961 atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa
    10021 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata
    10081 aggatataaa atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc
    10141 tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc
    10201 agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact
    10261 gtccatcatt accgccaaag acgaagacgg tctggagatc attcgtcact cctgtgcgca
    10321 cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt
    10381 tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt
    10441 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa
    10501 gaaagtcagc tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt
    10561 ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga
    10621 atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa
    10681 actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg
    10741 tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc agcgcctgga
    10801 agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca
    10861 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact
    10921 agaaatgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt
    10981 catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat
    11041 gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccgggtca
    11101 cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga
    11161 gtttggtagc tgccaccgta atgagccgtc aggttcgctg catggcctga tgcgcgtgcg
    11221 tggatttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt
    11281 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt
    11341 cgtcaaactc tccactcgtc ctgaaaaacg tattggcagt gatgaaatgt gggatcgtgc
    11401 tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg
    11461 tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc
    11521 atggcagtgc ggtacagtac agctggactt ctctttgcct tctcgtctga gcgcttctta
    11581 tgtaggcgaa gacaatgaac gtaaagtacc ggtaatgatt caccgcgcaa ttctgggatc
    11641 gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct
    11701 tgcgccagtt caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga
    11761 attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa
    11821 gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg
    11881 tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg
    11941 aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct
    12001 taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa
    12061 ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca
    12121 gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt
    12181 agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt
    12241 cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt
    12301 taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa aactccgcag
    12361 cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg
    12421 tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca
    12481 agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt
    12541 gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agccttcggg
    12601 cttcacaggc tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa
    12661 aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg
    12721 tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg
    12781 taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc
    12841 gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca
    12901 ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa
    12961 ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc
    13021 aggctgttat caaagctggt cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt
    13081 tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaacggt atttcttaca
    13141 gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg
    13201 atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc
    13261 tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa cgccatcaaa
    13321 acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt
    13381 aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca cctgaatcca
    13441 ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc
    13501 tttttttgta tgcgcgtttt gaaaaataaa aagcctgtct catcaggtgt aattgtcgca
    13561 gccagtctgg acacggccag cgcggagaaa ccggagcgta tttaagtacg tgagaatttc
    13621 gagcactgcc cgggaccaaa atggcaagta aaatagcctg atgggatagg ctctaagtcc
    13681 aacgaaccag tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg
    13741 ttgccagtgc gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc
    13801 gcgtcgaata tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc
    13861 tgccgccaga agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc
    13921 agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat gcgcgtctgg
    13981 cggcggaaac gattgatgtc tctctgccag gtcgtcgcat tgaaaacggc ggtctgcatc
    14041 cggttacccg tactatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg
    14101 caaccgggcc ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc
    14161 accacccggc gcgcgctgac cacgacactt tctggtttga cactacccgc ctgctgcgta
    14221 cccagacctc tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca
    14281 ttgcgcctgg ccgcgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc
    14341 agatggaagg tctgattgtt gataccaaca tcagctttac caacctgaaa ggcactctgc
    14401 acgatttcct gcgtaacttc tttgaggaag atttgcagat tcgcttccgt ccttcctact
    14461 tcccgttcac cgagccttct gcagaagttg acgtcatggg taaaaacggt aaatggctgg
    14521 aagtgctggg ttgcgggatg gtgcatccga acgtgctgcg taacgttggc atcgacccgg
    14581 aagtttactc tggtttcgcc ttcgggatgg ggatggagcg tctgactatg ttgcgttacg
    14641 gcgtcaccga cctgcgttca ttcttcgaaa atgatctgcg tttcctcaaa cagtttaaat
    14701 aaggcaggaa tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc
    14761 gattgatagc gatgcgctgg cgaatcaaat cactatggcg ggcctggaag ttgacggtgt
    14821 agaaccggtt gccggtagct tccacggcgt ggtcgttggt gaagtggttg agtgtgcgca
    14881 gcatccgaac gctgacaaac tgcgtgtgac aaaagtgaat gttggcggcg atcgcctgct
    14941 ggacatcgtc tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagctaccat
    15001 tggtgctgtt ctgccgggtg atttcaaaat taaagcggcg aaactgcgtg gtgaaccgtc
    15061 tgaagggatg ctgtgctcct tttctgagct gggaatttct gacgatcata gcggcattat
    15121 cgagctgcct gcggatgcgc cgattggcac cgacatccgt gaatacctga aacttgatga
    15181 caacaccatc gaaatcagcg tgacgccaaa ccgtgccgac tgtttaggta tcattggtgt
    15241 tgcgcgtgac gttgccgtgc tgaatcagct gccgctggtt gaaccggaaa tcgttccggt
    15301 tggtgcgacc atcgacgaca cgctgccgat tacagtcgaa gcgccggaag cctgcccgcg
    15361 ttatcttggc cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa
    15421 agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg tcaccaacta
    15481 tgtactgctc gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg
    15541 cattgtggtg cggatggcga aagagggcga aacgttggta ctgcttgatg gcactgaagc
    15601 gaagctgaat gctgacactc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg
    15661 catctttggt ggcgaacact ctggcgtgaa tgacgaaacg caaaacgtct tgctggaatg
    15721 cgctttcttc agcccgctgt ctatcaccgg tcgtgctcgt cgtcatagcc tgcatactga
    15781 tgcgtctcac cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg
    15841 tgcgacccgt ctgctgatcg acatctgcgg tggtgaggct ggtccggtaa ttgatatcac
    15901 caacgaagca acgctgccga agcgtgcaac catcacttta cgtcgtagca aactggatcg
    15961 cctgatcggc catcatattg cggatgagca ggtaactgac attctgcgtc gtctcggctg
    16021 cgaagtgacc gaaggcaaag acgagtggca ggcagttgcg ccgagctggc gtttcgatat
    16081 ggagattgaa gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc
    16141 ggatgagccg gtacaggcaa gcctgattat gggtactcac cgtgaagctg acctgtcgct
    16201 caagcgcgtg aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt
    16261 cgttgatccg aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctgccaag
    16321 cccgatctct gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac
    16381 cgtggtgtac aaccagaacc gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg
    16441 tttcgtacca gatactcagg caccgttggg cattcgtcag gatctgatgt tagccggtgt
    16501 gatttgcggt aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta
    16561 tgatttgaaa ggcgatcttg aatccgttct cgacctgacc ggtaaactga atgaggttga
    16621 gttccgtgca gaagcgaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa
    16681 aggtaaacgt attggttttg ttggggttgt tcatcctgaa ctggaacgta aactggatct
    16741 taacggtcgc actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc
    16801 tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc
    16861 agaaaacgtt cccgcagcgg atattttatc cgaatgtaag aaagttggcg taaatcaggt
    16921 agttggcgta aacttatttg acgtgtaccg cggtaagggt gttgcggagg ggtataagag
    16981 cctcgccata agcctgatcc tgcaagatac cagccgtaca ctcgaagaag aggagattgc
    17041 cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga ttccaggcat cattgaggga
    17101 ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat aagcttgggc
    17161 ttagcaagcg ggatgccaaa gaactggttg aactgttttt cgaagagatc cgtcgcgctc
    17221 tggaaaacgg cgaacaggtg aaactctctg gttttggtaa cttcgatctg cgtgataaga
    17281 atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg
    17341 tggtgacctt cagacccggg cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag
    17401 acgagtaatc tgatctaact aaaaaggccg ctctgcggcc ttttttcttt tcactgtcga
    17461 agagtcaccg taaaatcaac gccatgacac ttcagcagaa cggataccat gctgacactt
    17521 gcccgccaac aacagcgaca aaatattcgc tggttattat gcctgtcagt tttgatgctg
    17581 ctggcgcttc tcttaagcct ttgcgccggt gaacaatgga tctcgccagg tgactggttt
    17641 actcctcgtg gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct ggctgtattg
    17701 ctggttggtg cggcgctggc tatatccggc gctgtaatgc aggcgttgtt tgaaaatcct
    17761 ctggcagaac ctggactact tggcgtctct aacggcgcag gcgtggggct tatcgccgcg
    17821 gtattgcttg ggcaagggca actccccaac tgggcgctag ggctgtgtgc gattgctggc
    17881 gcgcttatca tcactttaat actcttacgt ttcgcccgtc gtcatctttc gaccagtcgg
    17941 ttattgctgg ctggcgttgc attagggatt atctgtagcg cactaatgac gtgggctatc
    18001 tacttttcca cctctgttga tttacgtcag ctgatgtact ggatgatggg cggttttggc
    18061 ggcgtagact ggcggcaaag ctggctgatg ctggcattga tccccgtgtt gttgtggatc
    18121 tgttgtcagt ccaggccgat gaatatgtta gcacttggcg agatctcggc gcggcaactg
    18181 ggtttacccc tgtggttctg gcgcaatgtg ctggtggccg cgaccggctg gatggttggc
    18241 gtcagtgtgg cgctggcggg tgctatcggc tttattggtc tggtgatccc acatattctt
    18301 cggttgtgtg gtttaaccga tcatcgcgta ttacttcccg gctgcgcgct ggcaggggcg
    18361 agcgcattgc tgctggccga tattgtagcg cgcctggcat tagctgccgc agagctgcct
    18421 attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta tctggttatt gttaaaagca
    18481 agacgttagc cgcaaaaaga cggtctatga ttaaaagcta agatttttac tgaccacacc
    18541 caggagaaac gatgcaagat tccattctga cgaccgtagt gaaagatatc gacggtgaag
    18601 tgaccacgct ggagaagtac gctggtaatg tgctgttgat tgtcaatgtc gcctcaaagt
    18661 gtggcttaac gccgcaatat gagcagttgg agaatattca gaaagcctgg gccgatcgag
    18721 gttttgtggt gctgggattc ccgtgcaacc agtttctgga acaagaaccg ggcagcgatg
    18781 aagagattaa aacttactgt accaccacat ggggggtgac gttcccgatg ttcagtaaga
    18841 ttgaagttaa tggcgaagga cgccatccgc tgtatcaaaa attgattgcc gcagcgccga
    18901 ccgcagtcgc gccggaagag agcggattct atgcccgtat ggtcagcaaa ggtcgtgcac
    18961 cgctgtaccc tgatgatatt ttatggaatt ttgagaaatt tttagttggc agggacgggt
    19021 tggtcatcca gcgtttttcc ccggatatga cgcctgaaga tcccattgtg atggaaagca
    19081 ttaaactggc gttggcaaaa taatgtctat tgtgatgcag ttacaggatg ttgcggaaac
    19141 tacccgcctg gggccgcttt ctggcgaggt tcgggctggg gagatcctgc acctggtggg
    19201 gccgaatggc gcgggtaaga gtaccttgct tgcacgaatg gccggaatga ccagcggtaa
    19261 gggaagcatt cagttcgcgg ggcaaccact ggaagcatgg tccgcaacaa aactcgcgct
    19321 gcatcgcgcc tacctttcac aacagcagac gccgccgttt gcaatgccgg tctggcacta
    19381 cctgacactg catcagcacg ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc
    19441 gctggctctt gatgacaaac tcggacgtag caccaatcaa ctttccggcg gtgaatggca
    19501 acgcgtacgt cttgctgcgg tggtgctgca aatcacacca caagccaatc ccgcaggtca
    19561 attgctgctt cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa gtgcgttaga
    19621 caaaattctg agcgctctgt gtcagcaagg actggcgatt gtgatgagca gtcacgatct
    19681 caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc
    19741 cagtggacgc agagaagagg tgctcacgcc gccaaatctg gcgcaggcct atgggatgaa
    19801 ttttcgccgt ctggatatcg aaggtcacag aatgctgatt tcgaccatct gatttcatta
    19861 gtatcttgaa aatcgggtaa ttacgacgct aaattaatgc aagaataaaa aacagaggaa
    19921 ccgtacggaa tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc
    19981 caccataaag caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt
    20041 ttgaacgacc agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg
    20101 cgcggtgtgg actgttcggg atttgtggtt gtaacgatgc gcgatcgttt cgatttgcag
    20161 ctgccccgag aaaccaaaga acaagcctct atcggcacgc aaattgataa agacgagttg
    20221 ctgcctggtg acctggtctt tttcaaaacg ggttccggac aaaatggttt gcatgtaggt
    20281 atttacgata ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc
    20341 tcacttgata atgtctattg gcagaaaaat ttctggcagg cgagacgaat ttagtatgtc
    20401 gaaaaatgga cggcgatgat acatcgccgt ccatttactc ctgaatacag tagtattttg
    20461 cagacaaaaa ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaggac
    20521 ccctgaaaaa gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc
    20581 ttatggcctc atccctttta gccggatact gaaaaacatc cttcgagagg gacggttacc
    20641 atgaagattt ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat
    20701 aaccagcaac tccttgtcgg cgttgagtta attacccatt tctccagtga agatggcacg
    20761 gtgcgtatcc cgacgagtcg ggttatagcg caacttaccg aggagcaaca ttggcaatta
    20821 ttttcagagc agttggaatt actaaaatca tgccaacatt tttttattca acataaactc
    20881 tttgcctggc ttaatttaac gccacaagtg gctacattat tattagagag ggataattac
    20941 gcaggcgaac tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat
    21001 ctcaatgaag gaaaagataa tcgcggtctt ttatccttat cgcaagtgta tccccttgtg
    21061 ttaggtaatt tgggagcagg caatagcaca atgaaagcgg tttttgatgg tcttttcacc
    21121 cgcgtcatgc tgtataaaag ttttattcag caacaaatca cgcaccgctc cttcgaaccg
    21181 tttattcgtg cgatccagtc gcaaatttcc ccttgttgca attgcatcat tgcgggaggc
    21241 attgatacgg cagaaatatt ggcacagatt acccccttcg atttccatgc tctccaggga
    21301 tgcctgtggc ctgccgttcc cataaatcag ataacgacgt tggttcagcg ataacccttc
    21361 tgtttgctgg tgtttaagac gagagtaacc gtctacacta tcaaacagga ggatctatga
    21421 ccctgtcttt tattacccgc tggcgcgatg aattgccaga aacctataca gcactttccc
    21481 ctacgccttt aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga
    21541 gtattccatc gtcgctgttt aaaaatgctg caggtgtctg gggcggcgaa accctactgc
    21601 ctggtatgtc accactggcg caggtttaca gtggtcatca gttcggcgtc tgggcgggcc
    21661 aactgggtga tgggcgcggc attttacttg gcgaacaact gcttgctgat ggcactacaa
    21721 tggactggca tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg
    21781 cggttttacg ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca
    21841 ttccgacgac ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg
    21901 tggagccagg cgcgatgctg atacgtgtgg caccaagtca tctgcgcttt ggtcatttcg
    21961 aacattttta ctatcgccgc gagccggaaa aggttcgtca gttagctgac tttgccattc
    22021 gtcattactg gtcacatctt gaagatgatg aggacaaata ccgtctctgg tttaacgatg
    22081 ttgtcgcacg taccgcatcg ttaattgccc aatggcagac ggtcggcttt gctcatgggg
    22141 tgatgaatac cgacaacatg tcgctgctgg ggctgacgct tgattacggg ccgtttggtt
    22201 ttcttgatga ttacgaaccc ggttttattt gtaatcactc ggatcatcaa gggcgttaca
    22261 gctttgataa tcaacctgcc gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt
    22321 caccatttgt tgccgtagat gccctgaatg aggccctgga cagctatcag caggttttgt
    22381 tgacgcatta tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg
    22441 ataacgcgct actgaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc
    22501 gcacattccg catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg
    22561 agtttattga tcgtgcggca tttgatgact ggtttgcccg ttatcggggg cgtttgcaac
    22621 aagacgaggt tagcgatagt gagcgtcagc agctgatgca aagcgttaac ccagctctgg
    22681 tgttgcgcaa ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg
    22741 aattgcaccg cctgcatgag gcgttgcgaa atcctttcag cgacagagct gatgactatg
    22801 tcagtcgtcc acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggctac
    22861 ttggtcaaca gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga
    22921 tcgataatca gtttgccatc cggacctaac agtgtctggc tggaaattcg ccgaataggc
    22981 tgcggggaag cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta
    23041 taacgcataa aaattaataa taattgatat aacaatgatt cttattatca atattatggc
    23101 tgaccagagc aagcagtaat gtgctgagaa gacaacaagt tgcgcagaaa tgcccatccc
    23161 atcaggaatg ggcacgcatt cagaagcggg tatctaccgc agaggcaagt ttttcgacca
    23221 ggcgttcggt atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg
    23281 gctgaccgcc gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag
    23341 caatcgccgt agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc
    23401 gacggtgctg cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat
    23461 caaactcgtg cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt
    23521 tgccgccacg cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct
    23581 gaccattttt gtctggcgag agaaacatat ggctggcgcg ggctgcgcgg atagcatcca
    23641 cggcaatccg cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgccg
    23701 aagccatttc acggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga
    23761 ttaaatcagc aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gtagggacac
    23821 ccagctcatt tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat
    23881 agctgccgtt taaatccgga tcggagatta gccctttcca gccgacaaca gttcgtggtt
    23941 tttcaaaata ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact
    24001 gcagacgggt ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa
    24061 tgaccaacag tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg
    24121 tgacatgggt ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta
    24181 ccaggctctc aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa
    24241 atttgttgct tctacggcag gactaccggg aagtgatgcg catcacgata aaccaatcgc
    24301 agctgatttc aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa
    24361 aataagctaa tgcactagtt ctctagtaca ttcggcggct aagccccatg atatcgagga
    24421 tcttggtggc aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt
    24481 tacggtacaa cgcttctact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc
    24541 ggctgttctc ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc
    24601 cgaacaattt atgctgaagc ggtttgagcg acgcgggtag caccagattg tccatatcgt
    24661 cggcaataaa ggggtagttt gccgcgcgga taccaaactg cattgccaga tacagactgg
    24721 tgggggtttt accacagcga gaaacaccga gcaggatcac ctgagcctgg tccagattgc
    24781 gcaacgaaat gccgtcatcg tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat
    24841 atttattgag attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca
    24901 gtttcatctc ttgttgtagc ggggcaacca gcgcctgaac aatatcctgg caaaagcctt
    24961 cactttgcaa gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc
    25021 gcacacctgt ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct
    25081 cattttcgac aaacggcagc gtaatgctgc tgatagtgac gggaaattgt gacattactg
    25141 cgtgtcctaa tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat
    25201 caacagcatt atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa
    25261 ttaaaaacgc acagaagcgt aggacgtaat gtctggttta taaaatgaac cttcaatttt
    25321 attttttatg aaaacagcat ttcattttta tggtttcgtt tataccgatg gtttatgtgg
    25381 aaattgtcga atagagcagg tttgcgcaac gctgggatca gtcttaaaaa gtaaaaaaat
    25441 atatttgctt gaacgattca ccgttttttt catccggtta aatatgcaaa gataaatgcg
    25501 cagaaatgtg tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat
    25561 ggctcgtcac cgctggtgct ttggtataac caactcggca tgaatgatgt agacagggtt
    25621 gggggcaaaa atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc
    25681 gttccgaatg gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc
    25741 gtaaaccagc gcatttatga actgctggat aaaacggata ttgacgatgt tacccagctt
    25801 gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg
    25861 gaaaacgcca tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa cgcctctttt
    25921 gcggtgcgct cctccgccac cgcagaagat atgccggacg cttcctttgc cggtcagcag
    25981 gaaaccttcc tcaacgttca ggggtttgac gccgttctcg tggcagtgaa acatgtattt
    26041 gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgatcaccgt
    26101 ggcgtggcgc tctccgccgg tgttcaacgg atggtgcgct ctgacctcgc atcatctggc
    26161 gttatgttct ccattgatac cgaatccggc tttgaccagg tggtgtttat cacttccgca
    26221 tggggccttg gggaaatggt cgtgcagggt gcggttaacc cggatgagtt ttacgtccat
    26281 aaaccgacac tggcggcgaa tcgcccggct atcgtgcgcc gcaccatggg gtcgaaaaaa
    26341 atccgcatgg tttacgcgcc gacccaggag cacggcaagc aggttaaaat cgaagacgta
    26401 ccgcaggaac agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa
    26461 caggccgtac aaattgagaa acactacggt cgcccaatgg atattgagtg ggcgaaagat
    26521 ggccacaccg gtaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt
    26581 caggtcatgg agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct
    26641 atcggtcatc gcatcggcgc gggtccggtg aaagtcatcc atgacatcag cgaaatgaac
    26701 cgcatcgaac ctggcgacgt gctggttact gacatgaccg acccggactg ggaaccgatc
    26761 atgaagaaag catctgccat cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc
    26821 atcgctcgtg aactgggcat tccggcggta gtgggctgtg gagatgcaac agaacggatg
    26881 aaagacggtg agaaagtcac tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg
    26941 gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga tctgccgttg
    27001 aaagtgatga tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac
    27061 gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc gtatgattgg cgtccaccca
    27121 cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg
    27181 atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc gtctgactga agggatcgcg
    27241 acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg
    27301 aacgaatatg ccaacctggt cggtggtgag cgttacgagc cagatgaaga gaacccgatg
    27361 ctcggcttcc gtggcgcggg ccgctatgtt tccgacagct tccgcgactg cttcgcgctg
    27421 gagtgtgaag cagtgaaacg agtgcgcaac gacatggggc tgaccaacgt tgagatcatg
    27481 atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg ttgaagaact ggcgcgtcag
    27541 gggctgaaac gtggcgagaa cgggctgaaa atcatcatga tgtgtgaaat tccgtccaac
    27601 gccttgctgg cagagcagtt cctcgaatat ttcgacggct tctcaattgg ctcaaacgat
    27661 atgacacagc tggcgctcgg tctggatcgt gactccggcg tggtgtctga attgttcgat
    27721 gagcgcaacg atgcggtgaa agcattgctg tcgatggcta tccgtgccgc gaagaaacag
    27781 ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc atgaagactt tgccgcatgg
    27841 ttgatggaag aggggatcga tagcctgtct ctgaacccgg acaccgtggt gcaaacctgg
    27901 ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg cggcgttcag tcgccgggtt
    27961 tatgtgatcc ccgaagatga aacttattca atctcttcac agacatcctg cgttaatcgc
    28021 cgcataatat cttttcttaa caaaaacttt tgtattttac ctgaggcagt gcgcggtagt
    28081 ttttcgatta ccacgatatg ttcaggatat ttatattttg cgacccgttt acggctaaaa
    28141 aaagccacca cctcttccag cgataatgaa tgatgcggcg ctttcagcac gacataagcg
    28201 catgatcgtt cacctaaacg ttcatcgggc attgcaacca cacaggcatc gtgaatttta
    28261 ggatgctgca ataaaatatc ttccacttca cggctgctaa tattttcgcc gccgcggaca
    28321 ataatatctt ttttgcgtcc ggtaattttt atatagccag cctcatccat acggcagaga
    28381 tcgccgctgt aataccagcc ttcttcatcc agcgcacggg cggttaattc aggttcatca
    28441 aaatatccca taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt
    28501 aaggtcttgc gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccatcg
    28561 gtgtgcataa agcgcgacaa aggatcatcg agattcacca ccgcatgcgg cgaactttct
    28621 gtggaaccat aaacacttaa taatttaatg ccgagctgct ggcattcacg cgccactttt
    28681 ttggggattg tggttccgcc gcaaagaaag aaacgcagcg ctgacaggtc cgcgggttgt
    28741 ttctctagta cattcaaaag atcatagaca aacggcgttg cgcctagcat acaggtgcaa
    28801 cgctgctgct caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg
    28861 cgagcaccaa ttaaaaatgg tgctgttacg ccatgcagaa atcccgttgc gtgaccaagt
    28921 ggcgcaggca tcataaagac atcctgccag gtcagattca gccgcacgca ataagcccgc
    28981 tcactggcga gaatattgtt atgcgttagc attacgccct ttggcagacc ctcggttccg
    29041 gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg ttatcgccgt gatcagtgag
    29101 gtattgtcgg cgataatctg acttaatgag agggaagagg tggcgggagc cagtttgtcc
    29161 acgccgacaa tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc
    29221 gggcgcgttt gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca cttattgagc
    29281 acccacacca gttctgcttc ccgccaggaa ggcaacagcg gcacggaaac cgcaccgatt
    29341 ttcaggcagg caagatagat aacggtaaat tcacaccagc caggcagttg aaatgcgatg
    29401 cgatcgcctg attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga
    29461 tcgagcgcgc tataggtgta cgatgcacca tgattatcga ccacggcaat tttgtctggc
    29521 atcgcacgag cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc
    29581 tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg tcactttcat gttggactct
    29641 cgaaacagaa catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa
    29701 aatcaatacc gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttaccgcaca
    29761 gcacccgaca ggtgccgcac tccagacaac cggcagaatc aaaatgaatg ttgccggcgt
    29821 catcctgctt ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat
    29881 cgggatttgc cgccagaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc
    29941 ctaatttgat gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat
    30001 gccatctttc agcaagttga tcagcccaat tttcttcgca tgtcccatga tcattttgcg
    30061 taccggctgg tttggtttgc cgtcaatggt gaacatctcg ttcatgatgt cggcgaccat
    30121 tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcggaaatg
    30181 ctgcatatcg cgcatgacgc agctttgttc cagctcacgt ttgtattgcg ccagactgct
    30241 ggcggagaaa tcctcgcgtt ctttggcggc gatcaccgtt gtggcggcag cctgagccga
    30301 tgcaatggct aaatccatgc cacgaactgt aaaacccaaa ttcaggcaga agcctgcggc
    30361 gtcaccaacg atcatcacgc catcgttaac catctgcggc accattgcca gaccgccttc
    30421 tggcaccata tgcccggaat attcaagcag tttgccgccg ctaattagtg ggcgaatggc
    30481 ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc
    30541 acccaatcca caaaccagcc ccaaggatat ggaatccttg ttggtataga gaaatccccc
    30601 gcccatcagg ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt
    30661 aatattaaag cgatcgttga tctgttctgg tgtgaggcca ataacctctt taacaccaac
    30721 agcgtaatga tgcggatcgg aagcgggaac cattcccagc gagcggccaa gcatcgagtt
    30781 aacgccatca gccagaatca ccacattcgc ttcgagaata tcatccccag cctgcacgcc
    30841 agtgaccttg tttccttcac gaaccagcgc atcgacgcga acgcctggga taaactgcgc
    30901 gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata
    30961 agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctttc
    31021 ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatactgc
    31081 aaagcctgga atgattgctt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt
    31141 acatccggca ctgtcgccgc gttctatcac caggacatcc agcccggctc gcgccatgac
    31201 cagtgcggca acgctaccag caacgcccgc accgaccaca atggcatcaa atttgtcttc
    31261 cgacatgcgt tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggga
    31321 attagcggct caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac
    31381 cgtagtcggc ataattgaag attggcgcat ttttatcttt attgatggcg acaatcactt
    31441 ttgcgccgtt gccgccaacc atatgctgga tctgcccgga gatccccagc gtcaggtaga
    31501 gatcggattt cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct
    31561 cgccttcggc aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt
    31621 ggaccatttt caggtcatcc tgcgccgcca gaccacgacc gacgccaacc acacgttttg
    31681 ctttgctcag gtccacgctg cttgcggctt tcgcacggcg ttcgcgacag agaatttcat
    31741 gacgcggagc aacataaggt accgtttctg tcgggcactg atgagaggta tcactggtgc
    31801 acggttcctg aacaccgggt gcaagggtaa tgatcgccag cgggctgttg atcttttcct
    31861 gagcgaacgc taacccgcca tacatccggt gttcggcgca aatgtgacca tcgataatat
    31921 ccaccgccgt ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgctgcca
    31981 gagctttacc acgtttcgtc gcggccaaca gcaacatagc ggggtgttta tctttcagca
    32041 gggcagcaat gctttcggcg taattttcag tgcgttgcag cgcgtcgttt tgctcaagaa
    32101 cataaataca ttttggacca taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa
    32161 tggcatacac ctgttggccc cattgctgag cgccgccaaa cagttcagca taacgttcag
    32221 gattatcgct aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt
    32281 cagttcaggg ctttcttcag atgttccgcc agctcggcaa tggcctccgg cgaatcgtta
    32341 tcgataatga tgtgcttacg ttctgtttgc ggcggtacgc gaatgccgac cagttcagca
    32401 agtggcgcgc tctggctcca gtcaatacca cttgcctgcc actgatttac cggtttttta
    32461 cccgcgccga gaatggcttt catcgaagga atgcgtggca cgttaatatc ggaggtgacg
    32521 cagagcacgg ctggaacaga gagttcaata acttcaacat catcttcaag cgtgcgttca
    32581 atcaccagtg tattgccctg acgctgaata gcactcactg cattaatcac cggaagttgc
    32641 agaatttctc cgaccagcaa gccagcctgc tgggcataaa ggtcgccgga accttcacca
    32701 aagatcagta aatcgaagcc gatcttttca actgctgctg ccagcgcttt tgcggtatcg
    32761 agaggcagtg catgttcaag ttgcgcatcc tgcaccaaat acaggctgtg cggcccgcgg
    32821 gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg
    32881 gctatctcat cgtcatctgt tgcaagctgg cttgcagctt caatggcatt gagatcgaac
    32941 tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt attctggagt gacaacaatg
    33001 tcctgttctt caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt
    33061 cgcatacgga accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg
    33121 gtgcggggtt ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
    33181 gatcgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt
    33241 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
    33301 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
    33361 tgtatcaacg ctgttttgat aatgccagtg aaacgctgtt tgtcgccggt aaaacgccac
    33421 ggctttcacg ttttgcaatt agcgatgatc caaaatggga gtctggacat cacgttcatg
    33481 acaatgaaac cgagctgatt tacgtcaaga aaggggtagc aaggtttacc atcgattctt
    33541 cgttatatgt cgcacatgcg gatgacattg tggtgataga acgcggcagg ctgcatgcgg
    33601 tggcctctga cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt
    33661 ttcagggggc tgaggaaaat cagctactgc aaccgcattc ttgtccggta attgccgcgg
    33721 ggcagggaaa agaagtcata aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa
    33781 gtaaaaatag ccaaacatct tcgttatagg atgcatttgc ctatacgtta gccattcttt
    33841 actacgaaaa ttttaaaaat gcttatcgtt cggagcaggg gtatattaaa aaagatgttc
    33901 tga
//
LOCUS       DAZGCS010000051        33740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGCS010000051 DAZGCS010000000
VERSION     DAZGCS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.051"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..781
                     /locus_tag="ACHK01_002495"
     CDS             <1..781
                     /locus_tag="ACHK01_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8097434.1"
                     /translation="NKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHV
                     LAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQR
                     KRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSV
                     STDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSM
                     HNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            971..1084
                     /gene="yhiY"
                     /locus_tag="ACHK01_002496"
     CDS             971..1084
                     /gene="yhiY"
                     /locus_tag="ACHK01_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO8097435.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1147..2214
                     /gene="yhiI"
                     /locus_tag="ACHK01_002497"
     CDS             1147..2214
                     /gene="yhiI"
                     /locus_tag="ACHK01_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8097436.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2211..4946
                     /gene="rbbA"
                     /locus_tag="ACHK01_002498"
     CDS             2211..4946
                     /gene="rbbA"
                     /locus_tag="ACHK01_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO8097437.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4946..6070
                     /gene="yhhJ"
                     /locus_tag="ACHK01_002499"
     CDS             4946..6070
                     /gene="yhhJ"
                     /locus_tag="ACHK01_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8097438.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6143..6418
                     /locus_tag="ACHK01_002500"
     CDS             6143..6418
                     /locus_tag="ACHK01_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO8097439.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6415..6774
                     /locus_tag="ACHK01_002501"
     CDS             6415..6774
                     /locus_tag="ACHK01_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO8097440.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6824..7684)
                     /locus_tag="ACHK01_002502"
     CDS             complement(6824..7684)
                     /locus_tag="ACHK01_002502"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO8097441.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7716..7985)
                     /locus_tag="ACHK01_002503"
     CDS             complement(7716..7985)
                     /locus_tag="ACHK01_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO8097442.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(7975..9483)
                     /locus_tag="ACHK01_002504"
     CDS             complement(7975..9483)
                     /locus_tag="ACHK01_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO8097443.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9476..10834)
                     /locus_tag="ACHK01_002505"
     CDS             complement(9476..10834)
                     /locus_tag="ACHK01_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO8097444.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRITRSTV
                     YA"
     gene            complement(10911..11192)
                     /locus_tag="ACHK01_002506"
     CDS             complement(10911..11192)
                     /locus_tag="ACHK01_002506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8097445.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(11189..11662)
                     /locus_tag="ACHK01_002507"
     CDS             complement(11189..11662)
                     /locus_tag="ACHK01_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8097446.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11687..12433)
                     /locus_tag="ACHK01_002508"
     CDS             complement(11687..12433)
                     /locus_tag="ACHK01_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8097447.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12632..13033)
                     /gene="nikR"
                     /locus_tag="ACHK01_002509"
     CDS             complement(12632..13033)
                     /gene="nikR"
                     /locus_tag="ACHK01_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO8097448.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13039..13845)
                     /gene="nikE"
                     /locus_tag="ACHK01_002510"
     CDS             complement(13039..13845)
                     /gene="nikE"
                     /locus_tag="ACHK01_002510"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO8097449.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(13842..14606)
                     /gene="nikD"
                     /locus_tag="ACHK01_002511"
     CDS             complement(13842..14606)
                     /gene="nikD"
                     /locus_tag="ACHK01_002511"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO8097450.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(14606..15439)
                     /gene="nikC"
                     /locus_tag="ACHK01_002512"
     CDS             complement(14606..15439)
                     /gene="nikC"
                     /locus_tag="ACHK01_002512"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO8097451.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15436..16380)
                     /gene="nikB"
                     /locus_tag="ACHK01_002513"
     CDS             complement(15436..16380)
                     /gene="nikB"
                     /locus_tag="ACHK01_002513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO8097452.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16380..17954)
                     /gene="nikA"
                     /locus_tag="ACHK01_002514"
     CDS             complement(16380..17954)
                     /gene="nikA"
                     /locus_tag="ACHK01_002514"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8097453.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18065..18652)
                     /gene="acpT"
                     /locus_tag="ACHK01_002515"
     CDS             complement(18065..18652)
                     /gene="acpT"
                     /locus_tag="ACHK01_002515"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO8097454.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18707..19756)
                     /gene="yhhT"
                     /locus_tag="ACHK01_002516"
     CDS             complement(18707..19756)
                     /gene="yhhT"
                     /locus_tag="ACHK01_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8097455.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            19888..21105
                     /gene="yhhS"
                     /locus_tag="ACHK01_002517"
     CDS             19888..21105
                     /gene="yhhS"
                     /locus_tag="ACHK01_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097456.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(21109..21666)
                     /gene="dcrB"
                     /locus_tag="ACHK01_002518"
     CDS             complement(21109..21666)
                     /gene="dcrB"
                     /locus_tag="ACHK01_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO8097457.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(21739..22404)
                     /gene="yhhQ"
                     /locus_tag="ACHK01_002519"
     CDS             complement(21739..22404)
                     /gene="yhhQ"
                     /locus_tag="ACHK01_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO8097458.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            22625..22870
                     /gene="tusA"
                     /locus_tag="ACHK01_002520"
     CDS             22625..22870
                     /gene="tusA"
                     /locus_tag="ACHK01_002520"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO8097459.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(22972..25170)
                     /gene="zntA"
                     /locus_tag="ACHK01_002521"
     CDS             complement(22972..25170)
                     /gene="zntA"
                     /locus_tag="ACHK01_002521"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO8097460.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(25244..25870)
                     /gene="yhhN"
                     /locus_tag="ACHK01_002522"
     CDS             complement(25244..25870)
                     /gene="yhhN"
                     /locus_tag="ACHK01_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO8097461.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            26011..26370
                     /gene="yhhM"
                     /locus_tag="ACHK01_002523"
     CDS             26011..26370
                     /gene="yhhM"
                     /locus_tag="ACHK01_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO8097462.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(26373..26642)
                     /gene="yhhL"
                     /locus_tag="ACHK01_002524"
     CDS             complement(26373..26642)
                     /gene="yhhL"
                     /locus_tag="ACHK01_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO8097463.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(26632..27228)
                     /gene="rsmD"
                     /locus_tag="ACHK01_002525"
     CDS             complement(26632..27228)
                     /gene="rsmD"
                     /locus_tag="ACHK01_002525"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO8097464.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            27378..28871
                     /gene="ftsY"
                     /locus_tag="ACHK01_002526"
     CDS             27378..28871
                     /gene="ftsY"
                     /locus_tag="ACHK01_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO8097465.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            28874..29542
                     /gene="ftsE"
                     /locus_tag="ACHK01_002527"
     CDS             28874..29542
                     /gene="ftsE"
                     /locus_tag="ACHK01_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO8097466.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            29535..30593
                     /gene="ftsX"
                     /locus_tag="ACHK01_002528"
     CDS             29535..30593
                     /gene="ftsX"
                     /locus_tag="ACHK01_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO8097467.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            30838..31692
                     /gene="rpoH"
                     /locus_tag="ACHK01_002529"
     CDS             30838..31692
                     /gene="rpoH"
                     /locus_tag="ACHK01_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO8097468.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            31963..33066
                     /gene="livJ"
                     /locus_tag="ACHK01_002530"
     CDS             31963..33066
                     /gene="livJ"
                     /locus_tag="ACHK01_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO8097469.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(33265..33648)
                     /gene="panM"
                     /locus_tag="ACHK01_002531"
     CDS             complement(33265..33648)
                     /gene="panM"
                     /locus_tag="ACHK01_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO8097470.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         8077 a         8736 c         9172 g         7755 t
ORIGIN      
        1 taataaagac gctgtgacgc catgtacgtt tcttggcgac caaaccggcg atcgatttag
       61 taatatctgt ggcgatcaat ttatcatcga tttgttaaaa cgcataatga gtatcaatga
      121 aaatgttcat gtattagctg gaaatcatga aacgaattgc aatggtaact atatgcagaa
      181 cttcacgcgc atgaaaccac ttgatgagga tacctacgct gggataaaag attatcctgt
      241 atgtttctat ggccccaaat acaaaataat ggcaaatcat catgggataa cttttgatga
      301 tcagcgaaaa cgttacatta taggaccgat aacagtctct agtgatgaaa tgaccaatgc
      361 tctcgatccg gtcgaattag cggcaattat caataaaaag caccatgctg taattaatgg
      421 caaaaagttt aaaaatagcc gggctatctc ttgtcgatcc tttaatcgtt atttttctgt
      481 ttcgacagat tacagaccta aacttgaagc actattagca tgctcacaaa tgttaggtat
      541 aaatcaagtt gttgcacata acggtaatgg tggaagagaa tgtataggtg aaacggggac
      601 agttttaggt cttaacgccc gtgacagtaa acatgctgga agaatgttca gcatgcataa
      661 ttgccaaatt aaccctggtg cagggcctga gataaccact ccctggaagt cttaccagca
      721 tgaaaaaaac agaaatggac ttatgccttt gattagaagg cgcacaatgt tacagctctg
      781 aaccatagac aacatgaata ttaatgttac tatctggttg agactgttga taaaacgtaa
      841 aaaggaatgc tctgttcctg caactgaaca ggcgtgaaac gcgatccaac aggatggata
      901 ccgttttgtc agccagtttt atagatcaca cttatgagta tgcgtgtttc atttacggcc
      961 aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc cgttggcgct
     1021 caatgctctg cgcgctggtc gtatttgtcg tttccttctt atcccggatg gaagaatccg
     1081 ctgaacgtcg ttcggcggca gttgtaacac gcgtcgtttg cttacaggga ttatggcggg
     1141 ccgattatgg ataagagtaa gcgccatctg gcgtggtggg ttgtcggggc actggcggtg
     1201 gctgccgtcg tggcgtggtg gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc
     1261 agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc
     1321 gacaccattc tggtgaaaga aggccagttt gttcgcgaag gtgaagtgct ggcgaagatg
     1381 gatactcgcg tgttgcagga acagcgactg gaagccatcg cgcaaatcaa agaggcacaa
     1441 agcgccgttg ccgccgcgca ggctttgctg gagcaacgac aaagcgaaac tcgcgccgcg
     1501 cagtcgctgg ttaatcaacg ccaggcagaa ctggactccg tagcaaaacg tcatatgcgt
     1561 tcccgttcac tggcccaacg aggggctatt tctgcgcaac agctggatga cgaccgcgcc
     1621 gccgctgaga gcgcccgagc tgcgctggaa tcggcaaaag ctcaggtttc tgcttctaaa
     1681 gccgctatag aggcggcacg taccaatatc attcaggcgc aaacccgcgt cgaagcggca
     1741 caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa agccccgcgt
     1801 gatggacgcg tgcagtatcg ggttgccgag ccaggggaag tgctggcggc aggcggtcgg
     1861 gtgctgaata tggtcgatct cagcgacgtc tatatgacct tcttcctgcc aaccgaacag
     1921 gcgggcacgc tgaaactggg tggtgaagcc cggctgatcc tcgatgccgc gccagatctg
     1981 cgtattccgg cgaccatcag ttttgtcgcc agtgtcgccc agttcacgcc aaaaaccgtc
     2041 gaaaccagcg atgaacggct gaaactgatg ttccgcgtca aagcgcgtat cccaccggaa
     2101 ttactccagc agcatctgga atatgtcaaa accggtttac cgggcgtagc gtgggtgcgg
     2161 gtgaatgaag aacttccgtg gcctgacgac ctcgtggtga ggttgccgca atgacgcatc
     2221 tggaactggt tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag cattatggaa
     2281 aaaccgttgc gctgaacaat attactctcg atattccggc ccgctgtatg gtcgggctga
     2341 ttggtccgga tggcgtcggg aagtcgagct tattgtcgtt gatttccggt gcccgcgtca
     2401 ttgagcaggg caatgtgatg gtgctgggcg gcgatatgcg cgacccgagg catcgccgcg
     2461 acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaacctc taccacacct
     2521 tgtcggtgta tgaaaacgtc gattttttcg ctcgcctgtt cggtcacgac aaagcggagc
     2581 gggaagtgcg aattaatgag ctgctgacca gcacggggtt agcaccgttt cgcgatcgtc
     2641 cggcagggaa actctccggc gggatgaagc aaaaacttgg gctgtgctgc gcgttaatcc
     2701 acgacccgga actgttgatc cttgatgagc caacaacggg ggttgacccg ctctcccgcg
     2761 cccagttctg ggatctgatc gacagtattc gtcagcggca gagcaatatg agcgtgctgg
     2821 tcgccaccgc ctatatggaa gaggccgaac gcttcgactg gctggtagcg atgaatgccg
     2881 gagaagtgct ggcaactggc agcgccgaag agctacggca gcaaacgcaa agcgctacgc
     2941 tggaagaagc atttataaat ctgttaccgc aagcgcaacg ccaggcgcat caggcggtag
     3001 tgatcccacc gtatcaacct gaaaacgcag agattgccat cgaagcgcgc gatctgacca
     3061 tgcgttttgg ttccttcgtt gccgttgatc acgttaattt ccgcattcca cgcggggaga
     3121 tttttggatt ccttggttcg aacggctgcg gtaaatccac caccatgaaa atgctcaccg
     3181 gactgctgcc cgccagcgaa ggtgaggcgt ggctgttcgg gcaaccggtt gatccaaaag
     3241 atatcgatac ccgccgtcgg gtgggctata tgtcgcaggc gttttcgctc tataacgaac
     3301 tcaccgtgcg gcaaaacctt gagttacatg cccgtttgtt tcacatcccg gaagcggaaa
     3361 ttcccgcaag agtggctgaa atgagcgagc gttttaagct caacgacgtt gaagatattc
     3421 tgccggagtc attgccgctc ggcattcgcc agcggctttc gctggcggtg gcggtgattc
     3481 atcgcccgga gatgttaatc ctcgatgagc ctacttctgg tgtcgatccg gtggcgaggg
     3541 atatgttctg gcagttgatg gtcgatctct cgcgccagga caaagtgact atcttcatct
     3601 ccacccactt catgaacgaa gcggaacgtt gcgaccgcat ctcactgatg cacgccggaa
     3661 aagtgcttgc cagcggtaca ccgcaggaac tggttgagaa acgcggagcc gccagtctgg
     3721 aagaggcatt tatcgcctat ttgcaggaag cggcagggca gagcaacgaa gccgaagcgc
     3781 cgcccgtggt acacgacacc acccacgcgc cgcgtcaggg atttagcctg cgccgtctgt
     3841 ttagctacag ccgccgcgaa gcgctggaac tgcgacgcga tccagtacgt tcgacgctgg
     3901 cgctgatggg aacggtgatc ctgatgctga taatgggtta cggcatcagt atggatgtgg
     3961 aaaacctgcg ctttgctgtg ctcgaccgcg accagaccgt cagtagccag gcgtggacgc
     4021 tcaatctctc cggttcccgt tactttatcg aacagccgcc gctcaccagt tatgacgagc
     4081 ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcgat tgagatcccg cccaatttcg
     4141 ggcgcgatat cgcgcgtggt acgcctgtgg aactcggcgt ctggatcgac ggagcgatgc
     4201 ccagtcgtgc cgaaacggta aaaggttacg tgcaggcaat gcaccagagt tggcttcagg
     4261 atgtggcgag ccgacaatcg acacccgcca gccaaagcgg gctgatgaac attgagacgc
     4321 gttatcgcta taacccggac gtaaaaagcc tgccagcgat tgttccggcg gtgatcccgc
     4381 ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagc
     4441 tagggtcgat tatcaacctt tacgtgaccc ccaccacccg tagcgaattt ttgcttggca
     4501 aacagctgcc gtacatcgcg ctggggatgc tgaacttttt cctgctctgc ggcctgtcgg
     4561 tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac gctcaccctg gcggcgctgc
     4621 tgtatatcat cattgccacc ggaatggggc tgctgatctc cacctttatg aaaagccaga
     4681 ttgccgccat tttcggaacg gcgattatca cattgatccc ggcgacacag ttttccggga
     4741 tgatcgatcc ggtagcttcg ctggaagggc ctggacgttg gatcggcgag gtttacccga
     4801 ccagtcattt tctgactatc gcccgcggga cgttctcgaa ggcgctggat ctgactgatt
     4861 tgtggcaact ttttatcccg ttgctgatag ccatcccgct ggtgatgggc ttaagtatcc
     4921 tgctgctgaa aaaacaggag ggatgatgcg ccatttacgc aatattttta atctgggtat
     4981 caaagagttg cgcagtctgc tcggtgataa agcgatgctg acgctgattg tcttctcgtt
     5041 tacggtgtcg gtgtattcgt cagcgaccgt tacgcctgga tcgttgaacc tcgcgccgat
     5101 cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg
     5161 cccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg gactggacgc
     5221 cggacgctat accttcgcga taaatattcc gcctaatttt cagcgtgatg tcctcgccgg
     5281 acgccagccg gatattcagg tgaacgtcga tgccacgcgc atgagccagg catttaccgg
     5341 caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg
     5401 tgataacagc gaaccgttgg tatcgctgga aacccggatg cgctttaacc cgaaccttga
     5461 tcccgcgtgg tttggcgggg tgatggcgat catcaacaac attaccatgc tggcgattgt
     5521 attgaccgga tcggcgctga tccgcgagcg tgaacacggc acggtggaac atttactggt
     5581 gatgccgata acgccgtttg agatcatgat ggcgaagatc tggtcgatgg ggctggtggt
     5641 gctggtggta tcgggattat cgctggtgct gatggtgaaa ggtgtactgg gcgtaccgat
     5701 tgaaggctcg atcccgctgt ttatgctggg cgtggcgctc agtctgtttg ccaccacgtc
     5761 aatcggcatt tttatgggga cgatagcgcg ttcaatgccg caactggggc tgctggtgat
     5821 tctggtgctg ctaccgttgc aaatgctttc tggtggatcc acgccgcgcg aaagtatgcc
     5881 gcagatggtg caggacatta tgctgaccat gccgacgaca cactttgtta gcctcgcgca
     5941 ggccatcctc taccggggtg ccggattcga aatcgtctgg ccgcagttcc tgacgctgat
     6001 ggcaattggc ggcgcgtttt tcaccatcgc actgctgcga ttcaggaaga cgattgggac
     6061 aatggcgtaa ggaagggaaa cttgacttca tactgtgatg tcatactatt gatgtcataa
     6121 agtgaagtca taaggacatg ccatgagaac caaggtacag gctttacgga agaaacaaaa
     6181 aaatacattg gatcaaatat ttaaaactcc tgttcctcaa gggatcaagt ggtctgatat
     6241 agagtcactg gttaaagcat taggcggaga aattaaggaa ggaagaggtt cgcgttgtaa
     6301 gttcatacta aatatgagcg ttgcgtgttt ccatcggcct catccgtcgc cagataccga
     6361 taaaggcgct gtagaaagcg tgcgtgactg gttactaagt ataggggtaa aaccatgatc
     6421 aaactgaaga cgccaaactc tatggagatt gccgggcaac ctgcagttat cacttacgtg
     6481 ccagaactga atgcatttcg tggcaaattc ctgggcttgt caggttattg tgattttgtc
     6541 tctgacagca ttcaggggct gcaaaaagaa ggggaacttt ccctgctaga gtatcttgag
     6601 gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag aaaagattaa gacctttacg
     6661 ttacgctatc cagaatcgct gagtgaacgc cttaataatg cggctgcgca gcaacaagtc
     6721 tctgttaata cctatattat tgaaacactg aatgagcgtt tgaatcattt ataacttata
     6781 gcggcccaac aatttctgtt gcgccgctat atttttgtgg ctcttaagat gcctttctaa
     6841 ccaaccgatg cccctcattc atcatcagga ttttgctata cacatcttcc tcgacggcgt
     6901 tttttgctga tgccatgact cgtgccagat tcgcctcgtt gtgattattc acatacgctt
     6961 tgccgacggc ggtgataaac gctttgcgca gatcgctggc gatatttact ttagcgacgc
     7021 gataattaac aaagctgcgt aatatctccg gggcgatacc ggagccgccg tgaataacca
     7081 acggcaccgg acagacttcg gcaatgcgct tcagcagggg gatatcgata cgcggtatat
     7141 cgtccaggcc atgaacgttg ccaatagaaa ctgccagcat gtcgcaaccg gtacgctcga
     7201 caaaggtctt caccttttcc ggttccgttt tgcaatcggc ttcgctgaca tgatcatctt
     7261 ctttgccgag aatcgccccc agctccgctt caaccggtac accataggat ttacagaaat
     7321 cgacagcttc ctgggtgaaa gcgatattct cttcaaaaga gaatgcggca ccgtcaatca
     7381 tgaccgaggt aaagcccgcg cgcaccgcct gtttcacatc ctccagagtt ttcccgtgat
     7441 ccagatgaag actgaccgga acatccattt tatcggcgtg acgtttcacg atttcataaa
     7501 tgtaatcgta tcccgagagt tgtgcattgg ttggcgcaat ctgaatgaag ttgggtaatc
     7561 ccgaacgctc aatagcgttt aaaattgaaa ttgtcgtctc cagattggtg gtattaaatg
     7621 cgcccgctaa tacccgtttt tcctgaatgc ggtcgagcag tatccgtcca ttaactaatg
     7681 gcatattgaa aacctcatct tttaatatca gttaattatt cagcttcacc gaagcggtcg
     7741 cgaaataagt cggtgagagt ctgtatggcc tcgttttcat catcaccgtc gcagcgtatt
     7801 tccaccgttt ttccgccttt tattccggcg cgcataatat tcatcatgct ggtggcatta
     7861 atatcattac cctcatagtg cagcgtcact ttacagctat attttttggc ggctttcgcc
     7921 agacaggccg ccggacgtgc atgcaggcca gtactgtttc tgacctccac ggttttattc
     7981 aacatagtgt tcctcccgat gatgtaatgg catcacgcct tcttgttgta atttcaactg
     8041 ataccagctt ttccatttgt gctgataacg ctgtaattga tggtatctcg cccgattcgg
     8101 taacaaacgt tgggctatag cgactggcgg cggtggaata tccggtgcgg cggttaataa
     8161 tgctccgaca gaaaccgcct cgttaaatgc aattgcttca accggacgct gtaaaatatc
     8221 cgcctttaat tgcagccaaa gaggattatg ggttgccgga ccgatcactt ttaacgcgct
     8281 ggtgccaggc acgttaaaac aggcgaagca gttggcaaat tccatggtta atcccagtga
     8341 tacgctggca attaacatgt cgatacttaa cgtatcgcca agcccataaa ataatccacg
     8401 ggtatggcga tttttatagg gcgaacccga accgcgaaga tgtggaataa agacgggaat
     8461 gtcatcgagc aaccaattcc ccgccagata atcagcatgt ccgcgagtaa gtgatgcggc
     8521 gatctcctca tcggttagcc ggaacgtgtt gcgcagccac tccagcgcga aaccgcccac
     8581 gggcagcgac gcaaacaggg tgaactggtt ggcaagtgag taacagccgt ttgccagctt
     8641 gtcacgtttg gcctgttcat ccagcgtcgg ttgtgtatcc agttgcagaa tgccttccgt
     8701 ggtccccgtc gagttaagga tatcgcctgg catcatctgc aacgctcgcg cacccaccat
     8761 atggtcatgt ccggccagcg tcacgctgac cggttgcgag aacccaagcg tcttgcaaag
     8821 tgactcgctg acccatccgg cgtgctcgcc tggctgcacc aatggcgcaa atgccgtaca
     8881 gggaacgtgt aacaacgccg ccgctttcgc tgaccattcg cctttgcgca cgtccagaca
     8941 cagcgtgcgg ctggctaagg tgatatccgt gcgctgttcg ccagtcagcc gccagagcag
     9001 cacttccggc gcatgtagcc agcgcaggcc gcggcgatta cgcagcgggt aatgttccag
     9061 caaccagcgc attttgaagg cagagtaatt gctgtgtagt ggtagaccgc agatgtcata
     9121 aagtgctgca ctgtctgcct cgctaagcgt tgccagatac tcttcaccgc gacggtcata
     9181 ccatgccagc attggcgtca ggatctcgcc atgctcgtca aggaacacgc ctgattcgcc
     9241 aaaactggca atgctgatcc gtctgactgg cagcggcgaa gcggcgttca gttgcgctat
     9301 cgcctggcgg acctcctgcc agagggcgtc gatatcgaaa tcgacatcgc cctgtgggga
     9361 gatctgcttt gccgtcacga atttatgcgc gcccaacgtc gttgcgtcca ggcaggaaaa
     9421 gcaggtgact ttgcaattgg tggtgccaat atcgataacg atagctgcgc tgttatcagg
     9481 cataaaccgt gctccgcgtt attcggaaaa tgccatacag taccagggcg ataaccggca
     9541 ccagaccaat ggcgatgata ttgccgttga agatttccag cagccagaag cggaacgggt
     9601 taccgccatc aaggaaactg gaaacctggc cgctggagcc ttcggcaaat ttgaagttaa
     9661 cgtctttcgc catgccggta ataaacggtg cgattttagt cgcaatccac aggtcggcaa
     9721 taatcaccgg gatcgccgcc agaacggtgc ggaaaatatt gccacggctg gctaaagcaa
     9781 tcatcgacgc cattaccgcc aggttggcaa ggtcgccaag cggcaggact tcattacccg
     9841 gaaggacaaa cgcgataaag accgagatag gcgtcagcag cagggcggtg gagataatcg
     9901 ccgggttacc tacggcaacg gcgatatcca ggccgatata gagatcgtca cggtcagggt
     9961 atttggcatt gagatacttt ttaatggctt cagacagcgg taataaacct tccatcagga
    10021 tgcgtaccat gcgtggcagg atgaacatca caccgccaat gctgatgcca agcagcaaca
    10081 cttttttgaa atcgtatcct gcaatcacgc cgagcagtat gcccagaata gtgccaacca
    10141 tcatcggttc gccgaagatg ccaaactttt tctgtacggt ttccgggtcg atatgaatac
    10201 gattgaggcc ggggatatgg tcgatgattt tgtcgaccag cagaccgacc gggaagaaca
    10261 ccaccgaaga gagcgtcggc aatgagatcc cttccagtcc aaagtatttt tgtaccagtg
    10321 gcgcagacca gtcggcgagt ttaagtacta ccaccgcagc aatcgccgcc gccagcaaac
    10381 caaagaacag gctgccggtg ctgtaataaa ccaacgtacc agcaagggca aaatgccagt
    10441 agttccacat atctacgtca acggtacggg ttttatttaa cgccagcatc gccacgttaa
    10501 tcagaatggt cagggggata acaaacgggg caattggcga accccatgta atagcggaaa
    10561 gcggcggcca gcctaaatct accaccggta aattaattcc ggtgcgttca accatcgcct
    10621 gggccgctgg cccgacattg ctggtcagaa tatcgaaaat ggcataaata ccgacaaaac
    10681 caatgccgac tgttaatgac gaacgtaagg ctttagcggg ttttacttta aaacagagag
    10741 caagaataaa taacaccaac ggcaacatta ccgtcgggcc aaatcccaat atatattgca
    10801 caatattata gagggtatgc gcgatatcgt tcataataaa cctctttttc aggcgagagc
    10861 gcacctcgcg ttagcgcctg atggagtggg ttgaaattaa ttattattaa ttaattttta
    10921 aggatcgaaa caatttgcgc cagtactttc tcttcgccaa taccggtaat aatcggcaag
    10981 ccgttaacta ccgggacgtc gagttcataa ggcactttag tcgttgagac taccagtgct
    11041 acgccatcag tatttccagg taaagaggca acgttggttt gggaataatt gacgttaaga
    11101 ccgtgctctt tacaatattc catgactttt tctgttaccg ccgtggatgt ggcaataccc
    11161 gtggcgcaga caaataaaat ggtttgactc atatttcacc tcgttctttc attttgttta
    11221 agattactgt gtgtatttct gttgtttcca tcgcaagcag ggcttgcata aagggcgtat
    11281 cctgaatcat ttccataatc cagcccaggg catttaattg tttattactt tcgcttaagg
    11341 cgagcaaaaa aatcaccctg acaggtacgg ggtcaggatc gccgcccata tcggcaaact
    11401 ctaccggttc cggcaggatg ccgacggcaa ttgcattgtg cctgacatgt ttatggtccg
    11461 tgtgcggaat agcgacgcac ggtggttcgg taggcagccc cgttgggtac gtcgcctcac
    11521 gtttcagtac gtgatcggca aaatcaggga cgaccagctc ggcggcaacc aacggttgcg
    11581 ctaactgacg gatgatctgt tcaacgctta accctgccgg aagtcgttga atataatttt
    11641 cctgaaaatg tatgccttgc attttactcc tcttaaaaaa gtgtacttaa ctcacttccc
    11701 gccgttgcag tacggtgcca agataatatt tatttccttt taaccagacg ttgccaaact
    11761 caatgggtaa tcctcgggag aaaaagacta actgctccag atgcaaaacg ggtgcgtctt
    11821 cactgatatc taaaaaatga ccacgttcat taccaattaa tcgcgcggca tagcgacttt
    11881 ccgagtaacg aatttttctt tgcgacaaac tttctattgt tggaaataaa ttgtgttgat
    11941 taaaatcgat ttccgcaata ccggggcata gctcaatatt gatacggttc tctatcagca
    12001 tcgctttttc atcaccaatt gagcgtaagc gttcaaggta aagaatatcc tgaccgggcg
    12061 ttattcttaa tttctctgcc acataacgat tagccgattc aatccgtgac gtaataactt
    12121 cagtggtaaa atgtattttc tggctttcca gcgattccgc gaatgacaat aatccttcac
    12181 ctaacggata tgccacgttc tcttttttga caaatgttcc cttaccctgg acctgtatta
    12241 acacgccttc ttttaccagt tgggcgaccg cttttttaac ggttccccga ctcacttcca
    12301 gcatatccat aatctggtat tccgaaggaa tgcgatcgtc ttccaccaga tctccggtat
    12361 aaatactttc tcttatccac tccaccactt gttgatagag cggtatttgt gacgacttac
    12421 ttaatgattt catggcgcgc acctgaacat ttacagttgt ctatggacaa tcatggacaa
    12481 cttcaagcgt atagtaggtg aacgggagaa aaagaggatc gaagcgtctc acattttgag
    12541 ggaccaaatc gtgatcaaaa aaatgtgacg tgagtcgata tttgtcagaa gtttactgcg
    12601 ccgtaaggaa ataacggcgc gttggtcggt gttaatcttc cttcggcaag cactgcaaat
    12661 gcccgtgccg cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc acgtcaccca
    12721 tgtcaccttt caacacggcg atttccagac agtcgtcgtg gttgatgtgc acatgcagcg
    12781 tggcgacgga gaggtcgtgg tgatgatgct gggtggaaac aatacggctg gctaagtcgc
    12841 gtttttcgtg ttcatacaca tacgacagca ccgcgaaacc ttgtgtgccg tgctgctggg
    12901 tagcttcttg cgccagggcg ctacgcagaa tatcgcggat agcttcggaa cggttgttat
    12961 aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca agcgtgatgg
    13021 tgactcgttg catttgcgtt aaaccttttc tgtggtgcga cggcgcacgg ggaatgcggg
    13081 taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaggttaatt tatctcccac
    13141 cacctgggtt tcgacgattt gtccgttgtc catcaccatc acccgctgac agaagcgttc
    13201 caccaggcgt aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt gttgtagctt
    13261 tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca aggttagaaa cggcttcatc
    13321 cagaatcagt agtttcggtt cgaccgccag cgcgcgagcc aggcagacgc gctggagctg
    13381 gccgccgctt aactgcggtg ggcgtttgtc gagaacgctg tcatcgagat cgaccgcctt
    13441 cagcatttcg ctggcgcgcg ccagttgttc ggattttttc agtgatagca ggtggcgcat
    13501 cggttcacgc aggatctcgc gcacggtttt gcgcggattc acggcgctga tggagtcctg
    13561 aaataccatc tgaatatcgc ggcggaacgc tttacgctgg gcgcgattga gtttcgccag
    13621 cggttcgccg cgccagctaa tattcccttg tgagggagac tctaagccta ccagcagccg
    13681 cgcgagggtg ctttttccgc agccgctgcg tcccagcagg gcgacggttt cgccgctttt
    13741 cagggtcagg gaaacgttat tcagcaccgc ctgatgttga tgttttccgc taaatccacc
    13801 gtgggcatag tgatgggaaa ggccgcagac gttaagtaag gtcatgatgc cagctccata
    13861 ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtca ccgtatgttt gggggcgtta
    13921 aacagcgttt ctacatcgcc ctgttcgaca attttacctt gtgacatcac cgccacgtca
    13981 tccgccagac gcgccacaac gcccatatca tgggtgacca gcagcattcc cggcgcttgt
    14041 ttttgcataa tgctttccag cagatcgagg atgcgcgcct gtgctaccac gtcgaggtcg
    14101 gtggtcggtt catcggcgat gataaacggt gattcacaca gcaccgccat cgcaatcatc
    14161 atgcgctgca acatgccgcc gctcatctcg aacgggtaca gcttcagcac gcgcgcggcg
    14221 ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc gggtttccct
    14281 aacgccaggc aggtttcccg cgcgtgggtg tgcatggtgt gcagtggatt aaaggcgctg
    14341 cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaagg cgaaaccggt
    14401 ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcaa aatgcccagc
    14461 gtcgcggcgc aggttaatga tttcccgctg ccgctaccgc cgactaacgc cagcacgcgc
    14521 ccgcgttgca gggttaacga tacaccgtgt accagcggct gcgcggcctg tagcgcgata
    14581 ttacgtagtt caatctgttg cggcattagt gtgcgtgctc cgtcaccaga tgagggtcca
    14641 gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata aacagcgcca
    14701 gccccggcca gaacatttgc agcggctggg tccagatata ctggcgcgcg tcgttaatca
    14761 tcacgcccca ttcggcggtc ggcgcggtca caccgaggcc aaggaaagac atccccgcga
    14821 cgtgcagcat catatggccg atatccagcg ttgccagcac cagcagcgaa gggatcaccg
    14881 cgcctgccag atgatcgaca aacacccgca catggcccgc gccagaaagc cgtgacgcca
    14941 gcacaaactc gcgttggcgt agtgaaatca ccaggctgcg caccatgcgt gcataccacg
    15001 cccagtgcga cagggcgatg gcgataatta cgttggtcag cccggtgccg agcacgccaa
    15061 ccataaagaa cgacagaatc gaggtcggga aggtcataaa catatcggcg acgcgcatgg
    15121 tggcctgatc aacgcgcccg ccaatcaacc cggcgctgcc gccaataacc agccctaatg
    15181 tcagcaccag cagcaggcag gccattaccg aaccgagcga cacgcgggtc gctgccatca
    15241 gccgcgagaa aatatcgcga cctaagtgat cggtgcccag ccagtgctgc gcatccggcg
    15301 aaagcaggcg cgacggcaaa tcaatcgcct gggggtcata cggcagccac cactggctgg
    15361 tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgtacc gaccagcggg
    15421 aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg ggtccagcgc
    15481 ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca tcatcagcgt
    15541 aaagcactgg atcaccggat agtcacggtt aaaaatcgcc gacaccgcat agcgcccgac
    15601 gcccggccag gcaaagatgt tttcgataat catcgtcccg ccaatcagtt cgccgatgtg
    15661 catccccacg gcggtgatca tcggcagcga ggcattgcgc aggatgtggc gacgttcggt
    15721 ctgtttgtcg ttcaggccgc gcagacgcgc ccaggtgacg tgacgctgac cggcgacgtc
    15781 cagcatactg gcgcgcagta aacgcgcgtt aatcgccagc gacataaagg caatggaaac
    15841 cgcaggcaaa atgatgtgct gccagccgcc gtaacccatc gcgggtagcc attgcagata
    15901 caccgaaaac gccatcacca gtaaaaacgc cagccagaag ttaggcatcg acacgccaag
    15961 aaacgcgatg aaacgtacgg cgaaatccgg cagacggtcg cgatggcgcg ccgcccagat
    16021 accgagcggt acggaagtga gcagaattaa taccagcgcc gcacctgcaa gttccagcgt
    16081 ggcgggcagg aagttcagca tatcgtccag caccgggcgt tggctggcga atgagatacc
    16141 aaagtcaaga tgcagcgcct tccacaacca ggtgccgtac tggacgtaca gcggctgatc
    16201 cagcccgagc atggtgcggg tagaggccag catctccggc gtcggcggca ggttagataa
    16261 acgcagataa tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa aaatgatcac
    16321 cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt aacgcaacat
    16381 taaggtttca ccggtttaat ctgttcgaac ggaatttcag tggcgatcgg cgcgtagggg
    16441 atgttaccca gctccggttt tgataccacc atcattgaga tgtaactgat aggcagataa
    16501 accgcctcgt catgcagacg ggtcagaatg tcgcgataca gcgcctgacg ttgcgtttcg
    16561 tcatgggtcg ccagcacttc gccgatctct ttatcaatca gcggtttgtc ggctaatcct
    16621 tgctgtgcct ggaagtcagc gtgtgacggt acgcgcattg aactgaggaa ggcgtgtgga
    16681 tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc gcgctgacga
    16741 gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat ctggcgcata
    16801 tcagcctgaa tgatttccgc catcgatttg cttaacgcat cggtgccgat gaacgacagt
    16861 tcaatgcgca gcggctgacc atttttctcg cggatgtctt tgcccgcagg cagcgtccaa
    16921 ccggcttttt ccagcaacgc tttcgctttc tgcggatcgt actggcgcgg tttcaggccg
    16981 aggttggcgt agggcacaga aggggcaaac agggtgtcgg cgacctgctg ggtgccatac
    17041 aacgcgttat caatcagcga ttttttgttt accgcgtaat taagagcttc acgtactgcc
    17101 agctcattgg tgggggcttt ggcggtattg agcgccagca tcacggtttc gatcggctgt
    17161 gacagttggg tgtggtaagc cggattctgg ctaaagcggg cgaaggtatc gagcggtaat
    17221 aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgccac cgcgcgggta
    17281 gtcgggtccg ggatgacgtt aaaggtgatc tttttaatcg ctggcttttc gccccagtag
    17341 ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgcaa aatccatggt
    17401 ccggtgccaa tcggcgcttt aattccgttc atggtttcat ggtttttaaa ctgcgaggga
    17461 gcgataaaac ggaaaggacg gggcagggcc agttcttgca ggaaaggata gtaggcgctt
    17521 ttcagggtaa tttgcagctc tgttttactg agtgctttaa catcaacaat ctggtttgcc
    17581 agctccagcc aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt ttctgccgcc
    17641 gcctcggcat cgaacggttc accgttggag aatttcacgt catcacgcag ggtgaaggtc
    17701 caggttttac catcttctga atgagtccag ctttttgcca gccacgggat caccgaaccg
    17761 tctgcctgat atttcaccaa tggttcataa accatgctct gggcgaacat ctggttaggc
    17821 gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtagt gatttcatct
    17881 ggtgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag agtgcggcgg
    17941 agtgtggaga gcatggtaac cccaatggat taaaatagat ggcggaaata agtatgacga
    18001 ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca gtcgggtggg
    18061 cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta aagggggtgg
    18121 gggtgcagat cgccaggctt aaattttcaa gctgacaatg gctgactgac agcgaggagt
    18181 gataggtgct gtctacgctg acgatttgcc aggcgctgcc gccacgctgt ttaacgatgg
    18241 cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga tgcacggcgt
    18301 ccatctcagc gtgttccccg aggctgaata cggcgttcgc cagccagcgc cagttggcgc
    18361 gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcactcaac agcagagcga
    18421 tatcgtcacc gctatggctt aagttgaacc atagcggcgt ttccggcgcg aatgcaggtt
    18481 tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa agcaatgcac
    18541 gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt aaacccggtg
    18601 gcagtggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac atatcagggc
    18661 caacgtttaa tggaaaatga aagtgcgtat cgtatcactt gtcgcctcat cccggtaacc
    18721 gacttttcgg tctgcccggc cccagtaaaa tcgccagttt gctgccacct ttcgtcgttt
    18781 ccatccagat tttacacacg ctggttaatg gcaccgaaag tagcatccct accgggccga
    18841 gcagccatcc ccagattaac aatgaaagaa ataccaccat ggtggacatc cccaggcgat
    18901 ggcccatcat ccgtggctct aaaatattgc cgatgaccat atggacaact aaaaacaatg
    18961 cgccgaccag aatacattcg taaataccat taaacagcag cacctgaatc attggcggta
    19021 cggcggaaat taccgcgccg atattgggca cgtagttaag caaaaacgcc agtactgccc
    19081 acatcagcgc aaactgtacc cccatcagct ccagccccag ccagacgatg acacctgtcc
    19141 ataaactgag tagcgtcttc aatgcaagat agtgcgaaac gcctttaagt gcgcggtgta
    19201 atcccgcgat gtgaatctgt gggttattca gcgcaaaacg cattttgtaa gggacgtggc
    19261 gcacttcaaa cagcataaaa actacggtca tcaccagcaa aagcacgctc gccattgccc
    19321 cggaaagccc ggtcattagc gctgtggtga aggtaaccac tttttccgag tccatccgct
    19381 gcagcattcg ctccggcgac atatgcaaat taagaaaagg caacatctcc tgcaatttaa
    19441 aaagtttgcg cgtcaactcc ttattaaact tcggcagcat agagataaat tcgttaaacg
    19501 atgccgccag tacgccgacc agcgcagtta gtgcgatcag catcaccacc accacaatcg
    19561 taatggcaat ggggcgttgt actccccgac gaataaacca ggtgacgagc gggttgagga
    19621 cgatggcaaa aaacagcgcc agtaacagct gcacaatgat atctgccgct gcgtgaatgc
    19681 ccgcgaggat cactaccagc gaggccagct tgagcagaat gtgcatgccc gttttatcgg
    19741 gttgaggggt ttccattggg gcttccttgt gactttttgt attaagtgta gcgggagtca
    19801 cgccagcaat attctgattc ttactgctga ttttcacgtc agcacgtggt aaaagtgaaa
    19861 cactgttgta aaaatgtggt gatcctcatg cccgaacccg tagccgaacc cgcgctaaac
    19921 ggattgcgcc tgaatttgcg cattgtctct atagtcatgt ttaacttcgc cagctacctc
    19981 accatcgggt tgccgctcgc tgtattaccg ggctatgtcc atgatgtgat gggctttagc
    20041 gctttctggg caggattggt tatcagcctg caatatttcg ccaccttgct gagccgtcct
    20101 catgccggac gttacgccga tttgctgggc cccaaaaaga ttgtcgtctt cggtttatgc
    20161 ggctgctttt tgagcggtct gggatatctg acggcaggat taaccgccag tctgcccgtc
    20221 atcagcctgt tattactttg cctgggacgc gtgatccttg ggattgggca aagttttgcc
    20281 ggaacgggat cgaccctgtg gggcgttggc gtggttggct cgctgcatat cgggcgggtg
    20341 atttcgtgga acggcattgt cacttacggg gcgatggcga tgggtgcgcc gttaggcgtc
    20401 gtgttttatc actggggcgg cttgcaggcg ttagcgttaa tcattatggg cgtggcgctg
    20461 gtggccattt tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg
    20521 ccgtttcgcg cggtgcttgg gagcgtctgg ctgtacggta tggcgctggc actggcttcc
    20581 gccggatttg gcgtcatcgc cacctttatc acgctgtttt atgacgctaa aggttgggac
    20641 ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc
    20701 cctaacggca ttaaccgtat cggtggctta aacgtagcga tgatttgctt tagcgttgag
    20761 ataatcggcc tgctactggt tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc
    20821 ttactggcgg gggccgggtt ttcgctggtg ttcccggcat tgggtgtagt ggcggtaaaa
    20881 gcggttccgc agcaaaatca gggggcggcg ctggcaactt acaccgtatt tatggattta
    20941 tcgcttggcg tgactggacc actggctggg ctggtgatga gctgggcggg cgtaccggtg
    21001 atttatctgg cggcggcggg actggtcgca atcgcgttat tactgacgtg gcgattaaaa
    21061 aaacggcctc cggaacacgt ccctgaggcc gcctcatcat cttaaaactt actgaataac
    21121 cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt tgctgatcgt cagcgggcag
    21181 cgtaatttgc atggtcagca gttgattacc cacgttaccc agaataacgg aagagtacgc
    21241 cgtctggcct ttcgcggaga taatactgtc taactgctgc attttgtgac ctttcagctc
    21301 aatggcttta ttggttacca cttgcagctg cggatcgcgg ctacgttgct gatcttccag
    21361 acgcttcgcc agcaccgcca gatcttcttt cggatcatcg cccatgatga cgatgactgc
    21421 tttctgcccg gtggcgtcgg accagacatg catgttattg gcctgcgttc ccagcttacc
    21481 gctctggtcg gtcatatccg ctggcagcga gaaacttaac ttgccatcaa gcaggttgac
    21541 gggattcccg gtagcgttac tttccgcgac cgaaccctgc gccgtagcgt tagtgtcttt
    21601 atcatcacag gccgcaagcc ccataaccag caggccaatt ccgacatatt taaccagatt
    21661 gcgcattgac ttcttccttt cgataaacgg ccataacggc tcattcatcc atcttatcac
    21721 aactctgata acgaaccttt aactcgcctg caaagcgttg atttcggatt tatctgccag
    21781 tcttttcaac agcatattga gtaatacgcc atacattggc aggaagaaaa cgatactgat
    21841 taacactttg aaacagtaat cgaccagcgc gatttccatc cagtgttcag ccataaaggc
    21901 atccgggcta cgccagaagg caatgaagaa aaaggccagc gtgtcactga cgttaccgaa
    21961 cagtgtggac gctgtcggtg ccagccacca gcggcgactt tgacgcaggc ggttaaaaac
    22021 gtgcacgtcg aggatttgcc ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc
    22081 gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc catgaaccca tatagaacag
    22141 cgacgagatg acataggaga ttaataacgc agggatcatt accgcgaaga taatgcgtcg
    22201 ggccagcggt gcgccaaaaa tacgcacggt caggtcggtc gcgagaaaga taaacggaaa
    22261 actaaacgcg ccccaggtgg tatgaaaacc aaaaatggag accggaagct ggaccagata
    22321 gttactggag gtgatcacca gcagatgaaa taacgataac cagaacaacg ccttatagcg
    22381 ttgagtttgc gagaaaacgt tcatattgta cctttttgat taaccattgg ggtgagggaa
    22441 cccaatacgt acgacacgtc tgttatctta acataataac gtgcttaccc ttttttcgag
    22501 ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aattttcacg caatcgttaa
    22561 cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttaa gtgatgagaa
    22621 gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcgcttg gcctgcgctg
    22681 cccggaaccg gtgatgatgg tgcgcaaaac cgtgcgcaat atgcagcctg gcgaaacttt
    22741 actgattatc gccgacgatc cggcgacgac ccgcgatatt cctgggtttt gtacctttat
    22801 ggaacacgaa ctggttgcga aagagacgga tggactgcct tatcgttatt tgattcgtaa
    22861 aggcggttga tggggaaaga ttggcttcga tgccgccttt tcccctcacc ctaaccctct
    22921 ccccagagga gcgaggggac cgatcgcgct caatgttgcg atcggtttgc cttatctcct
    22981 gcgcaacaat cttaacgcat tcgctgttac cagcacggtc gcaccggtat ctgccagcac
    23041 cgccagccat agtccggtca tccctagcag cgtggtgacg aggaagatcc ctttcagccc
    23101 cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat
    23161 ttgcaccagg ccgcgcaggt ggttatgggt taatgctgcg tcggcggttt ccagcgccac
    23221 gtctgtgcca ctgcccattg caatcccaat ggcggcagct ttcatcgctg gcgcgtcgtt
    23281 aataccatca ccgaccatcg ccaggggcgc atgttgattc agcttggtca ccgctttgac
    23341 tttatcttcc ggcaataggc ccgctttaaa ctccagcccc agctccccgg caattgccgc
    23401 cgctgcgcgt ggattatcgc cggtgaggat cactccttta acccccagcg cgttcagttc
    23461 actgatggca gttgctgcgt cggcgcgcag ggtatcctgc aatgcaatga tacccagcac
    23521 gtcatcatta cgtactacca gcacaaccgt ttgcccggcg ctttccagtt cattaatcaa
    23581 accagcaaat gcatcagcgg gatgtttccc ggcagcgcaa atcaatacgc gctcaccgtt
    23641 aacctgcgct tcaatgccag acccgaccag cgcccgctgt gattcggcgg tgggaatggc
    23701 gagttcagca acctgtgctt cgcgtacgat ggcctgcgcc agtggatgcg tcgcgccttg
    23761 ctcgaccgcc gccgccagtg tcagcagttc agattcacta atacccgttg ccggatgaat
    23821 cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg ccacctgagt
    23881 aacacgaccc agctgttcca gcgccgctcc gcctttaatc aacgccccac gacgcgctgc
    23941 cgccgccagc ccggaggtaa tcgccgcagg cgtggagata actaacgcac acgggcagcc
    24001 aatcagcagc agcgtcagcc ctttataaat ccactcctgc cagctggcgg caaacagcag
    24061 cggtggcacc agcgtcacca gcagggcgac ggccataatc gctggcgtat aaatacggct
    24121 gaaacggtcg ataaaccgct caatgggggc gcggcgctct tcggcttctt cgatcagttt
    24181 cagaatccgg tcaatggcgc tggcacccgg ttctgacagc acttccagcg ttaccagacg
    24241 gtctacgctg gtggctcctg caggaacttt atcgcccgtc gcgcgctcca ccggaatgga
    24301 ttctccggtc agggcgcttt catcaaaact ggcaaacggt gagagcagtt taccgtcggc
    24361 aggcaaacgc ccacctgcgg cgacttcaat cacatcgcca ggccgcaggc tgttaatcgc
    24421 cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt tctggtttca gcgccattaa
    24481 cgcgctaacg ccctgacgcg cgcggctggc ggcccagcct tccagtcgtt caccaatcaa
    24541 aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagtg caccaatagc
    24601 ggctacgctc attaaggttt caatggcgaa gtagctgccg gatttgatca gccgtaatgc
    24661 ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca gttgcccaaa
    24721 cggatgatta aattgctcca gaccccagct gattgccatc atcacgatta gcgtaatcag
    24781 cggcagattc tctttcaggc gcgatgcttg tggctcgtcg gcggcctgct catcgcgcag
    24841 ggaatagccc gctttttgca ccgcagattc aacctgggca cggatgtcat tgtcggcatc
    24901 gaccaccagt ttttcggtgg cgaacagtac ctgcacctga ttcacacctg caagctggcg
    24961 cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc atgccgctga ctttccagct
    25021 atagcgggtg ccggagacgt tttcagagag cgttggcgag ctggaacatg cgccgtcgca
    25081 gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg aacgcagcaa attgaggggc
    25141 tttcttgccg tgattgtcag gagtcgacat ggcatcctcc ggttaagttt tttctcatta
    25201 accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaga tacagcgagc
    25261 ggacgatgag gaaatgaccg gcaaagtagc aggccgcagc aatcgcgtta tctgcccgga
    25321 agcggcggcg atagtggcta cccagccaga caaagttact gataaacagc aatgaagcgc
    25381 caacaaacgc agagagtgcc ggagcggtcg gacggaagaa ccacagttca cctgccagcc
    25441 acaccatcac cagcgtcatg ccgataaacg tgcagatagg ccaacgatac tcttccagtc
    25501 gcgtccagat aatcgccagt aacagcgcgc ccagaaccag caacaccagt ggcagaggcc
    25561 agaagaaaga gagcgtcatc tgactggcga aatagatggt gtacagcagg tgcgagagga
    25621 aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc gcatcgccca
    25681 gcagtgaggc gcacagccct gccagcacca gatagctaat ggcgtcgaac atcggcgctt
    25741 gccaggccag taacagcagg agaagaaggg ttaacggttt aaacacccag cgttgccagg
    25801 ttggcccacg atacgatgca tccacagata gccatgcgga aagacagaca gcgataaacg
    25861 accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg cctacggtct
    25921 tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactggc agggtatgct
    25981 tatttccgca ttttccgatg agggataaag atgagcaaac cacctctttt ctttattgtt
    26041 atcattggct taattgtcgt cgccgcatcg tttcgtttta tgcaacagcg acgggaaaaa
    26101 gctgataatg atatggctcc gctccagcaa aagctggtgg tggtgagcaa caagcgggaa
    26161 aaaccgatta acgatcgccg ttcgcgccag caggaagtga ctccggcagg caccagtatg
    26221 cgctatgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac
    26281 gcccagcagt accacgccct gacagtgggc gataaaggta cgctgagcta taaaggaacg
    26341 cgctttgtca gctttgtagg cgaacaataa ggttattttt taactttaaa tttcttctgc
    26401 cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc
    26461 tgcgggccat ctttcggtaa agtggatttc aatagcgcca gttgcatacc atgcatcagc
    26521 acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa gggatgcacc
    26581 agattgagga ttaaaaatcc ccaaacgcag agcattaaca aacgaccaat attaatcagc
    26641 atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac ccgccacttt
    26701 ttcccgatgt aatgaccagt ttgccggaac agtgggcaga ccgttttcga cttcgctttc
    26761 gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat ttatcgtctc
    26821 ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg gtgtgccttt
    26881 ttgcgccagg aatgacattg cgttgctgtt caccacgcgt gcattgcctg cttttagtgt
    26941 cgccagattc ttaattaact gctgagaaac tgcgcgatcc atctcaatca acgtggcccc
    27001 cgcagcgtag cgcgataacg cttccagccc cagcgcgccg ctcccggcga agcaatccag
    27061 acattgggcg tcaacaatga ccggagccag ccagttaaac aacgtttcgc gtacgcggtc
    27121 ggtggtgggg cgcagacctg ggctatcagg aaccgggagt ttacggcctc gccactgccc
    27181 gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga taaattgctc
    27241 aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta
    27301 tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg aagcaacagc
    27361 gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct
    27421 ttggtcaaaa agagcagacc ccggaaaaag agacagaagt tcagaatgaa caaccggttg
    27481 tagaagaaat cgttcaggcg caagagcctg tgaaggcttc tgaacaagcc gtcgaagagc
    27541 agccgcaggc gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt gtggaagtca
    27601 ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc ggaagtcgtt gcacagccgg
    27661 aaccggtcgt agaagaaacg ccggagccag tggctatcga gcgtgaagag ctgccgttgc
    27721 cggaagacgt caacgccgaa gaggtttcgc cagaagagtg gcaggctgaa gcggaaaccg
    27781 tagagattgt cgaagcggcg gaagaagaag cggctaaaga agaaatcacc gacgaagagc
    27841 tggaagcaca ggcgctggct gccgaagcgg cagaagaggc ggtgatggtg gttcctccgg
    27901 tagaagagca gccggtggaa gaaatcgctc aggagcagga aaaaccgacc aaagaaggtt
    27961 tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga aaatctcggt tccggattta
    28021 tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt tgaggagctg gaagaacagc
    28081 tgttgatcgc cgatgtgggc gtggaaacca cacgtaaaat tatcaccaat ctgacggaag
    28141 gcgcatcccg caagcagctt cgtgatgccg aggcgctcta tggcctgctg aaagaagaga
    28201 tgggcgagat tctggcgaaa gtcgatgagc cgctgaatgt tgaaggtaaa acgccgttcg
    28261 tgatcctgat ggtgggcgtc aacggtgtgg gtaaaaccac gacgattggt aagctggcgc
    28321 gtcagtttga gcagcagggt aaatcggtga tgctggcggc gggcgatact ttccgtgcag
    28381 cagcggttga acagcttcag gtctggggtc agcgcaacaa tattccggtg attgcccagc
    28441 atactggtgc ggattccgcc tctgttatct tcgacgccat tcaggcagct aaagcgcgta
    28501 atatcgacgt cctgattgcc gataccgccg gacgtctgca gaacaaatcg cacctgatgg
    28561 aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga cgttgaagcg ccgcatgaag
    28621 ttatgctgac tatcgatgcc agcaccgggc agaacgcggt aagccaggcc aaactgttcc
    28681 atgaagctgt tggcttaacc ggcatcacgc taacgaaact ggacggcacg gcgaaaggcg
    28741 gggtaatttt ctcggtggct gaccagtttg gtatccctat ccgctacatt ggtgtcggcg
    28801 aacgtattga ggatttgcgt ccgtttaagg cggacgactt tatagaggca ctttttgccc
    28861 gagaggatta acaatgattc gctttgaaca tgtcagcaag gcttatctcg gtgggagaca
    28921 ggcgctgcag ggcgttacgt tccatatgca gccgggtgag atggcgtttc tgaccggtca
    28981 ttccggcgca gggaaaagta ccctcctgaa gctgatctgt gggattgagc ggcccagcgc
    29041 cgggaaaatc tggtttagcg gccatgacat cacgcgtctg aaaaaccgtg aagttccgtt
    29101 tctgcgccgc cagattggca tgattttcca ggatcaccat ctactgatgg accgtactgt
    29161 ctacgataac gtggcaatcc cgctgattat cgccggtgcc agcggtgacg atattcgtcg
    29221 ccgggtgtcg gcggcactgg ataaagtcgg gctactggac aaagcgaaga acttccctat
    29281 tcagctttct ggcggtgaac aacagcgtgt tggcattgcc cgcgcggtgg tgaacaagcc
    29341 cgcggtactg ctggcggacg aaccgactgg taacctggac gacgcgttgt cggaagggat
    29401 tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc gtattgatgg caacgcacga
    29461 catcaacctg atctcgcggc gttcctatcg catgctcacc ctgagcgatg gtcacttgca
    29521 tggaggcgtg ggccatgaat aagcgcgatg caatcaatca tattcggcag tttggcgggc
    29581 gtctggatcg cttccgtaaa tcggtcggcg gctctggcga cggcggtcgt aacgcgccaa
    29641 aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac caacgttttc aacgaacagg
    29701 tgcgctatgc cttccacggc gcattgcagg atctgaaaag caaaccgttc gccacgtttt
    29761 taacggtgat ggttatcgcc atctccctga cgctgcccag cgtctgttat atggtgtaca
    29821 aaaacgttaa ccaggcggcg acgcaatatt atccgtcacc gcaaatcact gtttacttgc
    29881 aaaaaacgct ggacgatgac gccgcagcag gcgtggtagc acagctacag gccgagcagg
    29941 gcgtggagaa agtgaactat ctttctcgtg aagacgcact gggcgagttc cgtaactggt
    30001 ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc gcttccggca gtggcggtgg
    30061 tgatcccgaa actcgatttt caggggacgg aatcactgaa tacgctgcgt gatcgtatca
    30121 cccagattaa cggcattgac gaagtgcgga tggatgacag ctggtttgcc cgtctggcgg
    30181 cgttgaccgg gctggtcggg cgcgtttccg cgatgatcgg cgtgttgatg gtggcagcag
    30241 tgttcctcgt catcggcaac agtgtgcgtc tgagcatctt tgctcgccgt gactccatta
    30301 acgtgcagaa actgattggt gcgacggatg gtttcatcct gcgcccgttc ctgtacggag
    30361 gcgcattgct gggattttct ggcgcattgc tgtcattaat tttgtcagaa attctggtac
    30421 tgcgtttgtc atcggcggtt gcagaagtgg cacaggtttt cggaacgaag tttgatatca
    30481 atggtttatc gttcgatgaa tgcctgctat tgctgctggt atgctcgatg attggctggg
    30541 tggcagcgtg gcttgccacg gtacaacatt tacgccactt tacgcctgaa taataaaagc
    30601 gtgttatact ctttccctgc aatgggttcc gtagcaggga aagagatccc gttgtctctt
    30661 cccggtattt catctctatg tcacattttg tgcgtaattt attcacaagc ttgcattgaa
    30721 cttgtggata aaatcacggt ctgataaaac agtgaatgat aacctcgttg ctcttaagct
    30781 ctggcacagt tgttgctacc actgaagcgc cagaagatat cgattgagag gatttgaatg
    30841 actgacaaaa tgcaaagttt agctttagcc ccagttggca acctggattc ctacatccgg
    30901 gcagctaacg cgtggccgat gttgtcggct gacgaggagc gggcgctggc tgaaaagctg
    30961 cattaccatg gcgatctgga agcagctaaa acgctgatcc tgtctcacct gcggtttgtt
    31021 gttcatattg ctcgtaatta tgcgggctat ggcctgccac aggcggattt gattcaggaa
    31081 ggtaacatcg gcctgatgaa agcagtgcgc cgtttcaacc cggaagtggg tgtgcgcctg
    31141 gtctccttcg ccgttcactg gatcaaagca gagatccacg aatacgttct gcgtaactgg
    31201 cgtatcgtca aagttgcgac caccaaagcg cagcgcaaac tgttcttcaa cctgcgtaaa
    31261 actaagcagc gtctgggctg gtttaaccag gatgaagtcg aaatggtggc ccgtgaactg
    31321 ggcgtaacca gcaaagacgt tcgtgagatg gaatcacgta tggcggcaca ggacatgacc
    31381 tttgacctgt cttccgacga cgattccgac agccagccga tggcaccggt gctctatctg
    31441 caggataaat catctaactt tgccgacggc atcgaagatg ataactggga agagcaggcg
    31501 gcaaaccgtc tgaccgacgc gatgcaaggt ctggacgaac gcagccagga catcatccgc
    31561 gcgcgctggc tggacgaaga caacaagtcc acgttgcagg aactggctga ccgttacggt
    31621 gtttccgctg aacgtgtgcg ccaactggaa aagaacgcga tgaaaaaact tcgcgccgct
    31681 attgaagcgt aatttccgct attaagcaga gaaccctgga tgagagtccg gggtttttgt
    31741 tttttgggcc tctacaataa tcaattcccc ctccggcaaa acgtcaatcc ccacgcagat
    31801 tgttaataaa ctgtcaaaat agctattcca atatcataaa aatggggtat gttttagcag
    31861 agtattctgc taaagcacgg gtagtcatgc ataaaacgaa ataaagtgct gaatcacaac
    31921 accacaacac acgtaataac cagaagaatg gggattctca ggatgaacat aaagggtaaa
    31981 gcgttactgg caggatgtat cgcgctggca ttcagcaata tggctctggc agaagatatt
    32041 aaagtcgcgg tcgtgggcgc aatgtccggt ccggttgcgc agtacggtga ccaggagttt
    32101 accggcgcag agcaggcggt tgcggatatc aacgctaaag gcggcattaa aggcaacaaa
    32161 ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac aggcggttgc ggtggcgaac
    32221 aaagtcgtta acgacggcat taaatatgtg attggtcacc tctgttcttc atcaacgcag
    32281 cctgcgtctg acatctacga agacgaaggc attttaatga tcaccccagc ggcaaccgcg
    32341 ccggagctga ccgcccgtgg ctatcagctg atcctgcgca ccaccggcct ggactccgac
    32401 caggggccga cggcggcgaa atatattctt gagaaagtga aaccgcagcg tattgctatc
    32461 gttcacgaca aacagcaata cggcgaaggt ctggcgcgag cggtgcagga cggcctgaag
    32521 aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg ccggggaaaa agatttctca
    32581 acgctggtgg cccgtctgaa aaaagagaat atcgacttcg tttactacgg cggttatcac
    32641 ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag ggctgaaaac tcagtttatg
    32701 gggccggaag gtgtggctaa cgtttcgctg tctaacattg cgggcgaatc agcggaaggg
    32761 ctgctggtga ccaagccgaa gaactacgat caggttccgg cgaacaaacc cattgttgac
    32821 gccatcaaag cgaaaaaaca ggacccaagc ggcgcattcg tttggaccac ctacgccgcg
    32881 ctgcaatctt tgcaggcggg cctgaatcag tctgacgatc cggctgagat cgccaaatac
    32941 ctgaaagcga actccgtgga taccgtgatg ggtccgctga cgtgggatga gaaaggtgat
    33001 ctgaaaggct ttgagttcgg cgtatttgac tggcacgcca acggcacggc gaccgatgcg
    33061 aagtaatcat taatcggcaa ctttgggttg ccgccaaatt gctaatatcg agtacgttgc
    33121 ttcatgccgg atgcggcgta aacgccttat ccggcctaca agatccaaag aaatcagtaa
    33181 attgcaacac acattgtagg cctgataagc gtagcgcatc aggcaatttc tcgttggtca
    33241 tcacttttga aatcaacttg acgattaacg cttctcccag ccgccctgtt gcgccgtaaa
    33301 ccccagcgcc tgcataaacg ccgtcatcac accgcgatct tccacgcctg catccgccat
    33361 ccaccagcat gaaacggcag gattgttacg caaaacctct tccagcagat attgccccac
    33421 accgcgacgg cgggtgactt cccgcacgcg cagggaatcc agtgctccct cggtgccgct
    33481 taaggttacc cgcacggccg ctagcaggcg ctcgttaaaa cgcgcggcgt agatgcggtg
    33541 gttatcgtca acctgtaaag aggaagggga atactccggc cagatctttt gcaggtcaat
    33601 ccggtcctgg tcgctaaagt tttctaatcg aatgatggtc agcttcatgg gtaacccgtg
    33661 taaatcacaa aagtggaacc agtgtagcga aataatttaa tcggaggctt tctctttttt
    33721 atttcttttg gcaggtgatt
//
LOCUS       DAZGCS010000052        31965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGCS010000052 DAZGCS010000000
VERSION     DAZGCS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.052"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            313..1881
                     /gene="cydA"
                     /locus_tag="ACHK01_002532"
     CDS             313..1881
                     /gene="cydA"
                     /locus_tag="ACHK01_002532"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO8097471.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1897..3036
                     /gene="cydB"
                     /locus_tag="ACHK01_002533"
     CDS             1897..3036
                     /gene="cydB"
                     /locus_tag="ACHK01_002533"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8097472.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3051..3164
                     /gene="cydX"
                     /locus_tag="ACHK01_002534"
     CDS             3051..3164
                     /gene="cydX"
                     /locus_tag="ACHK01_002534"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO8097473.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3164..3457
                     /gene="ybgE"
                     /locus_tag="ACHK01_002535"
     CDS             3164..3457
                     /gene="ybgE"
                     /locus_tag="ACHK01_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO8097474.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3607..4011
                     /gene="ybgC"
                     /locus_tag="ACHK01_002536"
     CDS             3607..4011
                     /gene="ybgC"
                     /locus_tag="ACHK01_002536"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8097475.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4008..4700
                     /gene="tolQ"
                     /locus_tag="ACHK01_002537"
     CDS             4008..4700
                     /gene="tolQ"
                     /locus_tag="ACHK01_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO8097476.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4704..5132
                     /gene="tolR"
                     /locus_tag="ACHK01_002538"
     CDS             4704..5132
                     /gene="tolR"
                     /locus_tag="ACHK01_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO8097477.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5197..6462
                     /gene="tolA"
                     /locus_tag="ACHK01_002539"
     CDS             5197..6462
                     /gene="tolA"
                     /locus_tag="ACHK01_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO8097478.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6595..7887
                     /gene="tolB"
                     /locus_tag="ACHK01_002540"
     CDS             6595..7887
                     /gene="tolB"
                     /locus_tag="ACHK01_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO8097479.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7922..8443
                     /gene="pal"
                     /locus_tag="ACHK01_002541"
     CDS             7922..8443
                     /gene="pal"
                     /locus_tag="ACHK01_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO8097480.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8453..9244
                     /gene="cpoB"
                     /locus_tag="ACHK01_002542"
     CDS             8453..9244
                     /gene="cpoB"
                     /locus_tag="ACHK01_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO8097481.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9409..9484
                     /locus_tag="ACHK01_002543"
     tRNA            9409..9484
                     /locus_tag="ACHK01_002543"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9442..9444,aa:Lys,seq:ttt)
     gene            9620..9695
                     /locus_tag="ACHK01_002544"
     tRNA            9620..9695
                     /locus_tag="ACHK01_002544"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9653..9655,aa:Val,seq:tac)
     gene            9698..9773
                     /locus_tag="ACHK01_002545"
     tRNA            9698..9773
                     /locus_tag="ACHK01_002545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9731..9733,aa:Lys,seq:ttt)
     gene            9923..9998
                     /locus_tag="ACHK01_002546"
     tRNA            9923..9998
                     /locus_tag="ACHK01_002546"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9956..9958,aa:Val,seq:tac)
     gene            10002..10077
                     /locus_tag="ACHK01_002547"
     tRNA            10002..10077
                     /locus_tag="ACHK01_002547"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10035..10037,aa:Lys,seq:ttt)
     gene            10224..10299
                     /locus_tag="ACHK01_002548"
     tRNA            10224..10299
                     /locus_tag="ACHK01_002548"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10257..10259,aa:Lys,seq:ttt)
     gene            10333..10408
                     /locus_tag="ACHK01_002549"
     tRNA            10333..10408
                     /locus_tag="ACHK01_002549"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10366..10368,aa:Lys,seq:ttt)
     gene            10657..11700
                     /gene="nadA"
                     /locus_tag="ACHK01_002550"
     CDS             10657..11700
                     /gene="nadA"
                     /locus_tag="ACHK01_002550"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO8097482.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11738..12457
                     /gene="pnuC"
                     /locus_tag="ACHK01_002551"
     CDS             11738..12457
                     /gene="pnuC"
                     /locus_tag="ACHK01_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO8097483.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12454..13395)
                     /gene="zitB"
                     /locus_tag="ACHK01_002552"
     CDS             complement(12454..13395)
                     /gene="zitB"
                     /locus_tag="ACHK01_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO8097484.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13509..13889)
                     /gene="ybgS"
                     /locus_tag="ACHK01_002553"
     CDS             complement(13509..13889)
                     /gene="ybgS"
                     /locus_tag="ACHK01_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO8097485.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14205..15257
                     /gene="aroG"
                     /locus_tag="ACHK01_002554"
     CDS             14205..15257
                     /gene="aroG"
                     /locus_tag="ACHK01_002554"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO8097486.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15415..16167)
                     /gene="gpmA"
                     /locus_tag="ACHK01_002555"
     CDS             complement(15415..16167)
                     /gene="gpmA"
                     /locus_tag="ACHK01_002555"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO8097487.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16372..17412)
                     /gene="galM"
                     /locus_tag="ACHK01_002556"
     CDS             complement(16372..17412)
                     /gene="galM"
                     /locus_tag="ACHK01_002556"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO8097488.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17406..18554)
                     /gene="galK"
                     /locus_tag="ACHK01_002557"
     CDS             complement(17406..18554)
                     /gene="galK"
                     /locus_tag="ACHK01_002557"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO8097489.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18558..19604)
                     /gene="galT"
                     /locus_tag="ACHK01_002558"
     CDS             complement(18558..19604)
                     /gene="galT"
                     /locus_tag="ACHK01_002558"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO8097490.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19614..20630)
                     /gene="galE"
                     /locus_tag="ACHK01_002559"
     CDS             complement(19614..20630)
                     /gene="galE"
                     /locus_tag="ACHK01_002559"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO8097491.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20891..22363)
                     /gene="modF"
                     /locus_tag="ACHK01_002560"
     CDS             complement(20891..22363)
                     /gene="modF"
                     /locus_tag="ACHK01_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO8097492.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            complement(22431..23219)
                     /gene="modE"
                     /locus_tag="ACHK01_002561"
     CDS             complement(22431..23219)
                     /gene="modE"
                     /locus_tag="ACHK01_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO8097493.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23348..23497
                     /gene="acrZ"
                     /locus_tag="ACHK01_002562"
     CDS             23348..23497
                     /gene="acrZ"
                     /locus_tag="ACHK01_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO8097494.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23664..24437
                     /gene="modA"
                     /locus_tag="ACHK01_002563"
     CDS             23664..24437
                     /gene="modA"
                     /locus_tag="ACHK01_002563"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8097495.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24437..25126
                     /gene="modB"
                     /locus_tag="ACHK01_002564"
     CDS             24437..25126
                     /gene="modB"
                     /locus_tag="ACHK01_002564"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO8097496.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSILHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25129..26187
                     /gene="modC"
                     /locus_tag="ACHK01_002565"
     CDS             25129..26187
                     /gene="modC"
                     /locus_tag="ACHK01_002565"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO8097497.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26188..27006)
                     /gene="ybhA"
                     /locus_tag="ACHK01_002566"
     CDS             complement(26188..27006)
                     /gene="ybhA"
                     /locus_tag="ACHK01_002566"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO8097498.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27161..28156
                     /gene="pgl"
                     /locus_tag="ACHK01_002567"
     CDS             27161..28156
                     /gene="pgl"
                     /locus_tag="ACHK01_002567"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO8097499.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28197..29150)
                     /gene="ybhD"
                     /locus_tag="ACHK01_002568"
     CDS             complement(28197..29150)
                     /gene="ybhD"
                     /locus_tag="ACHK01_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8097500.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKSLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            29334..30386
                     /gene="ybhH"
                     /locus_tag="ACHK01_002569"
     CDS             29334..30386
                     /gene="ybhH"
                     /locus_tag="ACHK01_002569"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO8097501.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30462..31895
                     /gene="ybhI"
                     /locus_tag="ACHK01_002570"
     CDS             30462..31895
                     /gene="ybhI"
                     /locus_tag="ACHK01_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO8097502.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         7680 a         7801 c         8419 g         8065 t
ORIGIN      
        1 atcaaaaaga gataaattgt tctcgatcaa attggctgaa aggcggtaat ttagctataa
       61 attgatcacc gtcgaaaaat gcaaatttgc ttcaacaaaa acctgtttat tgtaaggatt
      121 ttgcggcgta atatatacgt gggatcaatt tgagttttta ttaacatgtt tgcaaccttt
      181 ctttacgccg tttttgtgtg cattcacatg gtatgatgaa agtgttcaaa caaatttcta
      241 ttggggcatg cgtgtgaccc tttctaacgg ggttcactct cggagtcttc atgcgatgag
      301 caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct tacagtttgc cttgaccgcg
      361 atgtaccact tcctttttgt gccactgacg ctcggtatgg cgttcctgct ggccattatg
      421 gaaacggtct acgtcctctc cggcaaacag atttataaag atatgaccaa gttctggggc
      481 aagttgtttg gtatcaactt cgctctgggt gtggctaccg gtctgaccat ggagttccag
      541 ttcgggacta actggtctta ctattcccac tatgtagggg atatcttcgg tgcgccgctg
      601 gcaatcgaag gtctgatggc cttcttcctc gaatccacct ttgtaggtct gttcttcttc
      661 ggttgggatc gtctgggtaa agttcagcat atgtgtgtca cctggctggt ggcgctcggt
      721 tcaaacctgt ccgcactgtg gattctggtt gcgaacggct ggatgcaaaa cccaatcgcg
      781 tccgatttca actttgaaac tatgcgtatg gagatggtga gcttctccga gctggtgctt
      841 aacccggttg ctcaggtgaa attcgttcac actgtagcgt ctggttatgt gactggcgcg
      901 atgttcatcc tcggtatcag cgcatggtat atgctgaaag gtcgtgactt cgccttcgct
      961 aaacgctcct ttgctatcgc tgccagcttc ggtatggctg ctgttctgtc tgttattgtt
     1021 ctgggtgatg aatccggcta cgaaatgggc gacgtgcaga aaaccaaact ggctgctatt
     1081 gaagccgagt gggaaacgca acctgcgcct gctgccttta ctctgttcgg cattcctgat
     1141 caggaagagg agacgaacaa atttgcgatt cagatccctt acgcactggg catcattgca
     1201 acgcgttccg tggatacccc ggttatcggc ctgaaagagc tgatggtgca gcatgaagaa
     1261 cgcattcgta acgggatgaa ggcgtactct ctgctcgaac aactgcgttc tggttctacc
     1321 gaccaggcgg ttcgtgacca gttcaatagc atgaagaaag acctcggtta cggtctgctg
     1381 ctgaaacgct atacgccaaa cgtggctgat gcgactgaag cgcagattca acaggcaacc
     1441 aaagactcca tcccgcgtgt agcgccgctg tactttgcgt tccgtatcat ggtggcgtgt
     1501 ggcttcctgc ttctggcaat catcgcgctc tctttctgga gtgtcatccg caaccgcatt
     1561 ggcgagaaaa aatggcttct gcgcgccgcg ctgtacggta ttccgctgcc gtggattgct
     1621 gtagaagcgg gctggttcgt ggctgaatat ggccgccaac cgtgggctat cggtgaagtg
     1681 ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg atctcatctt ctcaatggtg
     1741 ctgatttgcg gcctgtatac cctgttcctg gtggcagaat tgttcttaat gttcaaattt
     1801 gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc actttgagca gtcttccacg
     1861 actactcagc cggcacgcta agacaggagt cgtcaaatga tcgattatga agtattgcgt
     1921 tttatctggt ggctgctggt tggcgttctg ctgattggtt ttgcagtcac tgacggtttc
     1981 gacatggggg tgggcatgct cacccgtttc ctcggtcgta acgacaccga gcgtcgaatt
     2041 atgattaact ccattgcacc acactgggac ggtaaccagg tttggctgat caccgcgggc
     2101 ggcgcactct ttgctgcctg gccgatggtc tatgccgctg cgttctccgg cttctatgtg
     2161 gcgatgatcc tcgtgctggc gtctttgttc ttccgtccgg tcggttttga ctaccgctcc
     2221 aagattgaag aaacccgctg gcgtaacatg tgggactggg gcatcttcat tggtagcttc
     2281 gttccgccgc tggtaattgg tgtagcgttc ggtaacctgt tgcagggcgt accgttcaac
     2341 gttgatgaat atctgcgtct gtactacacc ggtaacttct tccagttgct taacccgttc
     2401 ggcctgctgg caggcgtggt gagcgtaggg atgatcatta ctcagggcgc aacctatctg
     2461 caaatgcgta ccgtgggcga actgcacctg cgtacccgtg caacggctca ggtggctgcg
     2521 ctggtgacac tggtctgttt cgcactggct ggcgtatggg tgatgtacgg tatcgatggt
     2581 tatgtcgtga aatcgacaat ggaccattac gcagcctcta acccactgaa taaagaagtg
     2641 gttcgtgaag ctggcgcatg gctggttaac ttcaacaaca cgccaattct gtgggctatt
     2701 ccggcactgg gtgtggttct gccgctgctg accatcctga ctgcacgtat ggataaagcc
     2761 gcgtgggcgt ttgtgttctc ctccctgacg ctggcctgca tcatcctgac agccggtatc
     2821 gcaatgttcc cgtttgtgat gccgtccagc accatgatga acgcaagtct gacaatgtgg
     2881 gatgcaactt ccagccagct gacgcttaac gtcatgacct gggttgcggt ggttctggta
     2941 ccgatcattc tgctctacac cgcctggtgt tactggaaaa tgttcggtcg tatcaccaaa
     3001 gaagatattg aacgtaacac ccactctctg tactaagtaa ggagctaaaa atgtggtatt
     3061 tcgcatggat tctgggaacg cttcttgcct gttcgtttgg ggtaatcacc gcgctggcgc
     3121 ttgaacacgt cgaatcaggc aaagccggtc aagaagacat ctgatgagta agattatcgc
     3181 gactttgtat gcggtaatgg acaagcgccc cctgcgggcg ctttccttcg tgatggcgct
     3241 tctgttagca ggatgtatgt tttgggaccc atctcgtttt gccgcgaaga ccagtgaact
     3301 ggaaatctgg catggtttat tgctgatgtg ggccgtctgt gctggtgtga ttcacggcgt
     3361 gggctttcgt ccgcagaagg ttctttggca agggattttt tgcccattgc ttgccgatat
     3421 tgttctcatt gtcgggctga ttttcttctt cttttaaatc agaattctct ttaaaaaatt
     3481 atgggccgct ccaggcccat aaatttttac gctcccttaa cttgccctca ttcccaaacc
     3541 tcaatcgcgc gcgtatagta gcagcgttta aaagctctaa cttttgttgc attaccggga
     3601 tgtaaagtga atacaacgct gtttcgatgg ccggttcgcg tctactatga agataccgat
     3661 gccggtggtg tggtgtacca cgccagttac gtcgcttttt atgaaagagc acgcacagag
     3721 atgctgcgtc atcatcactt cagtcagcag gcgctgatgg ctgaacgcgt tgcctttgtg
     3781 gtacgtaaaa tgacggtgga atattacgca cctgcgcggc tcgacgatat gctcgaaata
     3841 cagactgaaa taacatcaat gcgtggcacc tctttggttt tcacgcaacg tattgtcaac
     3901 gccgagaata ctttgctgaa tgaagcagag gttctggttg tttgcgttga cccactcaaa
     3961 atgaagcctc gtgcgcttcc caagtctatt gtcgcggagt ttaagcagtg actgacatga
     4021 atatccttga tttgttcctg aaggctagcc ttctggttaa acttatcatg ttgattttga
     4081 ttggtttttc aatcgcatct tgggccatta ttatccagcg gacccgtatt cttaacgcag
     4141 cggcgcgcga agccgaagcg tttgaagata aattctggtc tggaatcgaa ctctctcgcc
     4201 tctatcaaga gagccagggg aaacgggata atctgactgg ttcggaacaa atcttttaca
     4261 gcgggttcaa agagtttgtg cgcctgcatc gtgccaatag ccatgcgccg gaagccgtag
     4321 tggaaggggc gtcgcgtgct atgcgtatct ccatgaaccg tgaacttgaa aatctggaaa
     4381 cgcacattcc gttcctcggt acggttggct ccatcagccc gtatattggt ctgtttggta
     4441 cggtctgggg gatcatgcac gcctttatcg ccctcggggc ggtaaaacaa gcaacactgc
     4501 aaatggttgc gcccggtatc gcagaagcgt tgattgcgac tgcaattggt ctgttcgccg
     4561 caattccggc ggtaatggct tataaccgcc tcaaccagcg cgtaaacaaa ctggaactga
     4621 attacgacaa ctttatggaa gagtttaccg cgattctgca ccgccaggcg tttaccgtta
     4681 gcgagagcaa caaggggtaa gccatggcca gagcgcgtgg acgaggtcgt cgcgatctca
     4741 agtccgaaat caacattgta ccgttgctgg acgtactgct ggtgctgttg ctgatcttta
     4801 tggcgacagc gcccatcatc acccagagcg tggaggtcga tctgccagac gctactgaat
     4861 cacaggcggt gagcagtaac gataatccgc cagtgattgt tgaagtgtct ggtattggtc
     4921 agtacaccgt ggtggttgag aaagatcgcc tggagcgttt accaccagag caggtggtgg
     4981 cggaagtgtc cagccgtttc aaggccaacc cgaaaacggt ctttctgatc ggtggcgcaa
     5041 aagatgtgcc ttacgatgaa ataattaaag cactgaactt gttacatagt gcgggtgtga
     5101 aatcggttgg tttaatgacg cagcctatct aaacatctgc gtttcccttg cttgaaagag
     5161 agcgggtaac aggcgaacag tttttggaaa ccgagagtgt caaaggcaac cgaacaaaac
     5221 gacaagctca agcgggcgat aattatttca gcagtgctgc atgtcatctt atttgcggcg
     5281 ctgatctgga gttcgttcga tgagaatata gaagcttcag ccggaggcgg cggtggttcg
     5341 tccatcgacg ctgtcatggt tgattcaggt gcggtagttg agcagtacaa acgcatgcaa
     5401 agccaggaat caagcgcgaa gcgttctgat gaacagcgca agatgaagga acagcaggct
     5461 gctgaagaac tgcgtgagaa acaagcggct gaacaggaac gcctgaagca acttgagaaa
     5521 gagcggttag cggctcagga gcagaaaaag caggctgaag aagccgcaaa acaggccgag
     5581 ttaaagcaga agcaagctga agaggcggca gcgaaagcgg cggcagatgc taaagcgaag
     5641 gccgaagcag atgctaaagc tgcggaagaa gcagcgaaga aagcggctgc agacgcaaag
     5701 aaaaaagcag aagcagaagc cgccaaagcc gcagccgaag cgcagaaaaa agccgaggca
     5761 gccgctgcgg cactgaagaa gaaagcggaa gcggcagaag cagctgcagc tgaagcaaga
     5821 aagaaagcgg caactgaagc tgctgaaaaa gccaaagcag aagctgagaa gaaagcggct
     5881 gctgaaaagg ctgcagctga taagaaagcg gcagcagaga aagctgcagc cgacaaaaaa
     5941 gcagcagaaa aagcggctgc tgaaaaggca gcagctgata agaaagcagc ggcagaaaaa
     6001 gccgccgcag acaaaaaagc ggcagcggca aaagctgcag ctgaaaaagc cgctgcagca
     6061 aaagcggccg cagaggcaga tgatattttc ggtgagctaa gctctggtaa gaatgcaccg
     6121 aaaacggggg gaggggcgaa agggaacaat gcttcgcctg ccgggagtgg taatactaaa
     6181 aacaatggcg catcaggggc cgatatcaat aactatgccg ggcagattaa atctgctatc
     6241 gaaagtaagt tctatgacgc atcgtcctat gcaggcaaaa cctgtacgct gcgcataaaa
     6301 ctggcacccg atggtatgtt actggatatc aaacctgaag gtggcgatcc cgcactttgt
     6361 caggctgcgt tggcagcagc taaacttgcg aagatcccga aaccaccaag ccaggcagta
     6421 tatgaagtgt tcaaaaacgc gccattggac ttcaaaccgt aatcgcgatg ttgactgttc
     6481 ggacggtcaa catcaggcac cggttgccac ggggttctgg tagttttgtg tattttagtt
     6541 tgttaacatt ctgctaaatt atcgtgggcc atcggtccag ataagggaga tatgatgaag
     6601 caggcattac gagtagcatt tggttttctc atactgtggg catcagttct gcatgctgaa
     6661 gtccgcattg tgatcgacag cggtgtagat tccggtcgtc ctattggtgt tgttcctttc
     6721 cagtgggcgg ggcctggtgc ggcacctgaa gatattggcg gcatcgttgc tgctgacttg
     6781 cgtaacagcg gtaaatttaa tccgttagat cgcgctcgtc tgccacagca gccgggtagt
     6841 gcgcaggaag tacaaccagc tgcatggtcc gcactgggca ttgacgctgt agttgtcggt
     6901 caggtcactc cgaatccgga tggttcttac aatgttgctt atcaacttgt tgacactggc
     6961 ggcgcaccgg gtactgtact tgctcagaac tcgtacaaag tgaacaagca gtggctgcgt
     7021 tatgctggtc ataccgccag tgatgaagtg tttgaaaaac tgaccggcat taaaggtgcg
     7081 ttccgtactc gtattgccta cgttgttcag accaacggcg gtcagttccc gtatgaactg
     7141 cgcgtatctg actatgacgg ttacaaccag tttgtcgttc accgttcacc gcagccgctg
     7201 atgtctccgg cgtggtcacc agacggttct aaactggctt atgtgacctt cgaaagcggt
     7261 cgttccgcgc tggttattca gacgctggca aatggcgctg tacgtcaggt ggcttcattc
     7321 ccgcgtcaca acggtgcacc tgcattctcg ccagacggca gcaaactggc attcgccttg
     7381 tcgaaaaccg gtagtctgaa cctgtacgta atggatttgg cttctggtca gatccgccag
     7441 gtgactgatg gtcgcagtaa caataccgaa ccgacctggt tcccggatag ccagaacctg
     7501 gcatttactt ctgaccaggc cggtcgtccg caggtttata aagtgaatat caacggcggt
     7561 gcgccacaac gtattacctg ggaaggttcg cagaaccagg atgcggatgt cagcagcgac
     7621 ggtaaattta tggtaatggt cagctccaat ggtgggcagc agcacattgc caaacaagat
     7681 ctggcaacgg gaggcgtaca agttctgtcg tccacgttcc tggatgaaac gccaagtctg
     7741 gcacctaacg gcactatggt aatctacagc tcttctcagg ggatgggatc cgtgctgaat
     7801 ttggtttcta cagatgggcg tttcaaagcg cgtcttccgg caactgatgg acaggtcaaa
     7861 ttccctgcct ggtcgccgta tctgtgataa taattaattg aatagtaaag gaatcattga
     7921 aatgcaactg aacaaagtgc tgaaagggct gatgattgct ctgcctgtta tggcaattgc
     7981 ggcatgttct tccaacaaga acgccagcaa tgacggcagc gaaggcatgc tgggtgccgg
     8041 cactggtatg gatgcgaacg gcggcaacgg caacatgtct tccgaagagc aggctcgtct
     8101 gcaaatgcaa cagctgcagc agaacaacat cgtttacttc gatctggaca agtacgatat
     8161 ccgttctgac ttcgctcaaa tgctggatgc acatgcaaac ttcctgcgta gcaacccgtc
     8221 ttacaaagtc accgtagaag gtcacgcgga cgaacgtggt actccggaat acaacatctc
     8281 cctgggtgaa cgtcgtgcga acgccgttaa gatgtacctg cagggtaaag gcgtttctgc
     8341 agaccagatc tccatcgttt cttacggtaa agaaaaacct gcagtactgg gtcatgacga
     8401 agcggcatac tccaaaaacc gtcgtgcggt actggtttac taagagaatt gcatgagcag
     8461 taacttcaga catcaactat tgagtctgtc gttactggtt ggtatagcgg ccccctgggc
     8521 cgcttttgct caggcaccaa tcagtagtgt cggctcaggc tcggtcgaag accgcgtcac
     8581 tcaacttgag cgtatttcta atgctcacag ccagctttta acccaactcc agcaacaact
     8641 ttctgataat caatccgata ttgattccct gcgtggtcag attcaggaaa atcagtatca
     8701 actgaatcag gtcgtggagc ggcaaaagca gatcctgttg cagatcgaca gcctcagcag
     8761 cggtggtgca gcggcgcaat caaccagcag cgatcaaagc ggtgcggcgg catcaacgac
     8821 gccgacagct gatgctggta ctgcgaatgc tggcgcgccg gtgaaaagcg gcgatgcaaa
     8881 cacggattac aatgcagcta ttgcgctggt gcaggataaa tcccgccagg atgacgcaat
     8941 ggtggcattt cagaatttca tcaaaaatta ccctgattca acttacctgc caaacgccaa
     9001 ttattggctg ggtcagttaa actacaacaa gggtaaaaaa gatgatgcgg cgtactattt
     9061 tgcttcggta gtgaaaaact atccgaagtc accaaaggct gctgatgcga tgtttaaagt
     9121 cggcgtcatc atgcaggaca aaggtgacac cgcaaaagcg aaagccgtat accagcaggt
     9181 tatcagtaaa taccctggta ctgatggcgc taaacaggca caaaaacgtc tgaacgcgat
     9241 gtaatgcata acacacgacc agaagtcgca ttatttctgg tcgtgtcgtg cgaatcataa
     9301 gcagttgagt gatctgcatc gaaatttttg ttgcgctcaa gtctgaaatc agtaatatat
     9361 gccgccgttg ccacgggata tcaaacaaac cgaaagcaac gaaaaagtgg gtcgttagct
     9421 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
     9481 accaatcgct aaggtggaag cggtagtaaa acgtgaagga taacgttgca tcagcaacgg
     9541 cccgaagggc gagacgaagt cgagtcatcc tgcacgaccc accactaaca tagttagttg
     9601 tagtatccag cgtagtattg ggtgattagc tcagctggga gagcacctcc cttacaagga
     9661 gggggtcggc ggttcgatcc cgtcatcacc caccaccggg tcgttagctc agttggtaga
     9721 gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccagttttag
     9781 catcgaagac agatgttaag cgtgtaggat aacgttgcgt cagcaacggc ccgtagggcg
     9841 agcgaagcga gtcatcctgc acgacccacc actaattacg gtgggttcgg tggaagtagt
     9901 ttgtagtatc cagcgcagta tcgggtgatt agctcagctg ggagagcacc tcccttacaa
     9961 ggagggggtc ggcggttcga tcccgtcatc acccaccact cgggtcgtta gctcagttgg
    10021 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatt
    10081 ttaacatcaa actcagatgt taagcgtgaa ggataacgtt gcgccagcaa cggcccgtag
    10141 ggcaagcgaa gcgagtcatc ctgcacgaca caccaatctt aaagattggc cccgagtaga
    10201 aatctttcag gtaacacccg tatgggtcgt tagctcagtt ggtagagcag ttgactttta
    10261 atcaattggt cgcaggttcg aatcctgcac gacccaccat cctgaatgat taaagcagca
    10321 taatcccgca aggggtcgtt agctcagttt gtagagcagt tgacttttaa tcaattggtc
    10381 gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg
    10441 ctattcaggc atcctcaatt tcactttgta aacctgatga catcgtcaga gcttactgtg
    10501 caagcaactc tacgtcggtg gaattaggcg taattcgagt gacttttccc caccattcga
    10561 ctatcttgtt tagcatataa aacaaattac accgataaca gcgaatatta cgctaatgtc
    10621 ggttttaacg ttaagcctgt aaaacgagat ggtaagatga gcgtaatgtt tgatccagac
    10681 acggcgattt atcctttccc cccgaagccg acgccgttaa gcattgatga aaaagcgtat
    10741 taccgcgaga agataaaacg tctgctaaaa gaacgtaatg cggtgatggt tgcccactac
    10801 tataccgatc ccgaaattca acaactggca gaagaaaccg gtggctgtat ttctgattct
    10861 ctggaaatgg cgcgcttcgg tgcaaagcat cccgcttcta ctttgttagt cgctggggtg
    10921 agatttatgg gagaaaccgc caaaattctc agtccggaaa aaacaattct gatgccgaca
    10981 cttcaggctg aatgttcact ggatctcggc tgccctgttg aagaatttaa cgcattttgc
    11041 gatgcccatc ccgatcgtac tgtcgtcgtc tacgccaaca cttctgctgc ggtaaaagcg
    11101 cgcgcagatt gggtggtaac ttcaagcatt gccgtcgaac ttattgatca tcttgatagt
    11161 ttgggtgaaa aaatcatctg ggcacccgac aaacatctgg ggcgttacgt gcaaaaacag
    11221 acgggtggag acattctatg ctggcagggt gcctgtattg tgcatgatga atttaagact
    11281 caggcgttaa cccgcttgca agaagaatac ccggatgctg ccatactggt gcatccagaa
    11341 tcaccacaag ctattgtcga tatggcggat gcggtcggtt ccaccagtca actgatcgct
    11401 gctgcgaaaa cattgccaca tcagcggctt atcgtggcaa ccgatcgggg cattttctac
    11461 aaaatgcagc aggcagtgcc agataaagag ttactggaag caccaaccgc aggtgagggt
    11521 gcaacctgcc gcagctgcgc gcattgtccg tggatggcca tgaatggcct tcagtccatc
    11581 gcagaggcat tagaacagga aggaagcaat cacgaggttc atgttgatga aaggctgcga
    11641 gagagggcgc tggtgccgct caatcgtatg ctggattttg cggctacact acgtggataa
    11701 cgaataataa ggcgtaacgt tacgctttgg gggaaagatg gattttttta gtgtgcagaa
    11761 tatcctggta catataccaa taggggcagg cggttatgat ctctcatgga tcgaagcggt
    11821 aggcacgatc gccgggttgc tgtgtattgg ccttgccagt ctggagaaga tcagcaacta
    11881 cttctttggc ctgatcaacg tcaccttgtt tggcattatt ttctttcaga ttcagctgta
    11941 tgccagcctg ctattacagg tgtttttctt tgccgcgaat atttacggtt ggtatgcgtg
    12001 gtcgcgacaa accagtcaga acgaggcgga gttgaaaatt cgctggttgc cattgccgaa
    12061 ggcactcagc tggttggcgg tttgcgttgt ttcgattggt ctgatgacgg tatttatcaa
    12121 tccggtgttt gcatttttga cccgcgtggc agtcatgatc atgcaagcat taggattaca
    12181 ggttgtgatg cctgaactgc aaccggacgc tttcccgttc tgggattcat gcatgatggt
    12241 gttatctatc gtggcaatga ttctgatgac gcgtaagtat gtggaaaact ggctgttgtg
    12301 ggtgattatt aacgtgatta gcgtcgttat ttttgcactt cagggcgttt acgccatgtc
    12361 tctggagtac atcatcctga cctttattgc gctcaacggc agccggatgt ggatcaacag
    12421 cgcacgtgaa agaggctcac gcgcgctgtc ccattaatgg tgatgatgtg aatggccaga
    12481 caccccttca ttcaggtggc agtctggccc atgacaaggt tgatattcca tctgaatggt
    12541 ggcgtgctca atctgatagt gatccatcag gtagtgttgg atctgatcca acaaggcatc
    12601 gtgatcgtgt ggcgggatca cctgcacatg caacgtcatc accggcttct cgcctaccat
    12661 ccatacatgt acatggtgca cattgcggac ttccgggatt tcccggcaca tacgacgctt
    12721 cagttcagcg atatccagcg ataccggtgc accttcaagt aattcattca cgctatcttt
    12781 caacagtcgc cacgcgctac gcagaaccag aagcgacacc agtatcgaga gaatggggtc
    12841 agcaggtgtc cagccagtcc agataataat caacgcggca ataatagccc caaccgaacc
    12901 cagcaggtcc ccgagcacat gcagtgccgc tgctcgtacg ttgaggtttt tctcttcgct
    12961 gccgtgatga agtaaccaga aagaaagtat atttgccagc aacccagcca cggcaattgc
    13021 catcatcatg ccgccctcga ccggacgcgg cgtgcggaag cgttctatcg cctcccagac
    13081 aatcaaaatg gtaattacca cgagggctat ggcattcaca aacgccgcga gcgtggtcag
    13141 tcttagccaa ccgaaagtgt ggcgaatggt gggaggacga cgtgaaaatt gcacggcgag
    13201 tagggcgaaa agcagggcgg cagtatcggt caacatatga cccgcatcgg ccagcaatgc
    13261 cagagaacca gaaagaaaac caccaacgac ttctaccagc ataaacccgg cagtcacgcc
    13321 gaaagcatac aacaagcgac gagcattatt atcttctggc aggtgtgagg acgtgtgtga
    13381 gtgtgagtgc gccatgacga gtcatccctt aattattctc acttaacttt atgacatcat
    13441 actgctttta gaagtgaaaa attaaaaggg agagactccg ctctcccatt attggctatt
    13501 ttgcagggtt actgcgtggt accgtcggtt ttggtatcga catcattatt gatgccatca
    13561 ccggtttgta cctttttatt gatatccgga cagcgaccat ctttgcacat ggtgttcttg
    13621 tgctcttcat ctttggtcat tccgtcattg ttcattgaag aaccatccga atgcagcatt
    13681 gtgccaccag aaccggtatt taccccgtta ttgtcgacgt tatttggcgc gacattttca
    13741 cgggcgtcag gggctacctg gcccgcatca gctgcggcgt ttgcctggcc gttattagtt
    13801 tgcgctccgc tatcggcagc cagtgcggca ccgctggcaa ggcttagagt ggcagtcaga
    13861 aataatgtgg ccagttttgt cattttcata ggatgctcct gttatggtcg ttatgtcgga
    13921 taacctcttc caacagtgca tttgcaggtg aatataaggc attggtttaa gatttcagcc
    13981 aggttatgaa acgcagcaga gaatcttgaa ataattaaca aacaaaggag ttacagttag
    14041 aaattgtagg agagatctcg tttttctcga caatctggcg tttttcttgc taattccagg
    14101 attaatccgt tcatagtgta aaaccccgtt tacacattct gacggaagat atagattgga
    14161 agtattgcat tcactaagat aagtatggca acactggaac agacatgaat tatcagaacg
    14221 acgatttacg catcaaagaa atcaaagagt tacttcctcc tgtcgcattg ctggaaaaat
    14281 tccccgctac tgaaaatgcc gcgaatacgg ttgcccatgc ccgaaaagcg atccataaga
    14341 tcctgaaagg taatgatgat cgcctgttgg ttgtgattgg cccatgctca attcatgatc
    14401 ctgtcgcggc aaaagagtat gccactcgct tgctggcgct gcgtgaagag ctgaaagatg
    14461 agctggaaat cgtaatgcgc gtctattttg aaaagccgcg taccacggtg ggctggaaag
    14521 ggctgattaa cgatccgcat atggataata gcttccagat caacgacggt ctgcgtatag
    14581 cccgtaaatt gctgcttgat attaacgaca gcggtctgcc agcggcaggt gagtttctcg
    14641 atatgatcac cccacaatat ctcgctgacc tgatgagctg gggcgcaatt ggcgcacgta
    14701 ccaccgaatc gcaggtgcac cgcgaactgg catcagggct ttcttgtccg gtcggcttca
    14761 aaaatggcac cgacggtacg attaaagtgg ctatcgatgc cattaatgcc gccggtgcgc
    14821 cgcactgctt cctgtccgta acgaaatggg ggcattcggc gattgtgaat accagcggta
    14881 acggcgattg ccatatcatt ctgcgcggcg gtaaagagcc taactacagc gcgaagcacg
    14941 ttgctgaagt gaaagaaggg ctgaacaaag caggcctgcc agcacaggtg atgatcgatt
    15001 tcagccatgc taactcgtcc aaacaattca aaaagcagat ggatgtttgt gctgacgttt
    15061 gccagcagat tgccggtggc gaaaaggcca ttattggcgt gatggtggaa agccatctgg
    15121 tggaaggcaa tcagagcctc gagagcgggg agccgctggc ctacggtaag agcatcaccg
    15181 atgcctgcat cggctgggaa gataccgatg ctctgttacg tcaactggcg aatgcagtaa
    15241 aagcgcgtcg cgggtaaggt ttaattgtcg gatgcgccgt cagagtggcg tatccgatga
    15301 atcaccacag gcctgataag tcgcgcagcg tcgcatcagg caatgtgctc cattgttagc
    15361 aacaaaaatg ccgactcact tgcagtcggc tttctcattt taaacgaatg acgtttactt
    15421 cgctttaccc tggtttgcaa ccgccgctgc tttcgctgcg atctcgtcag cattacccag
    15481 ataatagcgt ttcagcggtt tgaaattctc gtcgaactca tacaccagcg gcacgccagt
    15541 cgggatatta agctcaagaa tctcttcttc gctcatgtta tcaagatatt tcaccagcgc
    15601 acgtaaagag ttaccgtgtg cagcgatgat cacgcgctca ccgctcttca tacgcggcag
    15661 aatagtttca ttccagtaag ggatcacgcg gtcaatggtc agcgccaggc tttccgtcag
    15721 cggcagttct ttttcgctca gtttcgcgta acgcggatcg tgacccggat aacgctcatc
    15781 atctttagtc agttccggcg gagtcactgc aaaaccacga cgccactgtt tcacctgctc
    15841 gtcgccatac ttttcagcag tttccgcttt gttcagaccc tgcaacgcac cgtagtgacg
    15901 ttcgttcagt ttccaggatt tctcaacggg cagccatgcc tgatccagtt cgtccagcac
    15961 attccacagg gtatggatag cgcgtttcag cacagaggtg taagcaaagt caaagctgta
    16021 accttcctct ttcagcagct tacctgctgc ttttgcttcg cttacgcctt tctcagacag
    16081 atccacgtcg taccaaccgg tgaaacggtt ttctttgttc cactgacttt cgccatgacg
    16141 aaccagaacc agcttagtta cagccatata cttactcctc aaatcatttt ttaatgataa
    16201 taattctcat tatattgccg tgacgaagca acagcaatgc ttacgcataa ccatagcgaa
    16261 aatagtgtcg cagtgtaagg ttgttgtgaa tattgagttg caaatatgtc ggtgtttgct
    16321 ggtgatttga gcaatataag gataaagccc tcattacgag ggcttcaggt gctactcagc
    16381 aataaactga tattccgtca ggctggaata ctcttcgcca ggacgcagga agcagtccgg
    16441 ttgcggccat tcagggtggt ttgggctgtc cggcagaaac tcgctttcca gtgccaggcc
    16501 ttgccagtcg gcgtaaggtt cagttcctct tgatggcgtg ccgccgagga agttgccgga
    16561 gtagaattgc agagccggag cggtggtgta gaccttcagc tgcaattttt catctgctga
    16621 ccagacatgc gccgccactt tcttgccatc gcctttggcc tgtaacaaga atgcgtgatc
    16681 gtaacctttc actttgcgct gatcgtcgtc ggcaagaaac tcactggcga tgattttggc
    16741 gctacggaaa tcaaaagacg ttccggcgac agatttcagg ccgtcgtgcg gaatgccgcc
    16801 ttcatcaacc ggcagatatt cgtccgccag aatctgcaac ttgtgattgc gcacgtcaga
    16861 ctgctcgccg tcaagattga aatagacgtg attagtcata ttcaccgggc aaggtttatc
    16921 aactgtggcg cgataagtaa tagagatacg gttatcgtcg gtcagacgat attgcaccgt
    16981 cgcgccgaga ttacccggga agccctgatc gccatcatct gaactcaggg caaacagcac
    17041 ctgacgatcg ttctggttca caatctgcca gcgacgtttg tcgaaccctt ccgggccgcc
    17101 gtgcagctgg ttaacgccct gacttggcga aagcgtcacg gtttcaccgt caaaggtata
    17161 acggctattg gcgatgcggt tggcataacg accaatagag gcccccagaa acgcagcctg
    17221 atcctgatag cattccgggc tggcacagcc gagcagcgcc tcgcggacgc tgccatcgga
    17281 aagcggaata cgggcggaaa gtaaagtcgc accccagtcc atcagcgtga ctaccatccc
    17341 tgcgttgtta cgcaaagtta acagtcggta cggctgacca tcgggtgcca gtgcgggagt
    17401 ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt ctctttaata
    17461 cctgtttttg cttcatattg ttcagcgaca gcttgctgta cggcaggcac cagttcttcc
    17521 gggatcaacg cgacgataca gccaccaaat ccgccgccgg tcatgcgtac gccacctttg
    17581 tcaccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac ggtgatttcg
    17641 aaatcatcgc gcatagaggc atgagactcc gccattaact cgcccatacg tttcaggtcg
    17701 ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcggtcag gatatgacgc
    17761 acgcgtttcg ccacgatcgg gtccagttca tgcgcaacag cgttgaactc ttcaatggtg
    17821 acatcgcgca gagctggctg ctggaagaaa cgcgcaccgg tttcgcactg ctcacggcga
    17881 gtattgtact cactgccaac cagggtacgt ttgaagttac tgttgatgat gaccacagct
    17941 acgcctttcg gcatggaaac tgctttggtc cccagtgagc ggcagtcaat cagcaaggca
    18001 tgatctttct tgccgagcgc ggaaattagc tgatccatga tcccgcagtt acagccaaca
    18061 aactggtttt ctgcttcctg accattaagc gcgatttgtg cgccgtccag cggcagatga
    18121 taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga acttaacccg
    18181 gccccctgcg gcacattgcc gctgatcacc atgtccacac cgccgaagct gttgttacgc
    18241 agttgcagat gtttcaccac gccacgaacg tagttcgccc attgatagtt ttcatgcgcg
    18301 acaatgggcg catcgaggga aaactcgtcg agctgatttt cataatcggc tgccattacg
    18361 cgaactttac ggtcatcgcg tggcgcacag ctgatcacgg tttgataatc aatcgcgcag
    18421 ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac gcggccaggc
    18481 gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga ttgtgttttt
    18541 tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct gactgcgcgc
    18601 aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag catttcataa
    18661 ccaaccataa atttacgtac ggtggcggag cgcaacagag gcggataaaa gtgcgcgtgc
    18721 agctgccagt gttgattctc ttcgccatta aatggcgcgc cgtgccagcc catagagtag
    18781 gggaaggagc actggaagag gttgtcataa cgactggtca gctttttcaa cgccagtgct
    18841 aaatcgctgc gctgggcatc ggtcaaatcg gtgatccgta aaacgtgggc tttgggcagc
    18901 agtagcgttt cgaacggcca ggcagcccag taaggcacta cggctaacca gtgttcggtt
    18961 tcgacaacgg tacggctacc gtctgccagc tcgcgctgaa cataatccac cagcattggc
    19021 gatttctgcc cggcaaaata ttctttttgc aggcggtctt cgcgctcagc ttcgttaggc
    19081 aggaagctat ttgcccaaac ctgtccgtgc ggatgcgggt tagagcagcc catcgccgca
    19141 cctttgtttt caaagacctg cacccacggg tacgtttttc ccagctctgc ggtttgctcc
    19201 tgccaggttt tgacgatttc cgtcaatgcc gcaacgctca gttctggcaa cgttttactg
    19261 tgatccggtg aaaagcagat cacccggctg gtaccgcgcg cgctctggca acgcatcaaa
    19321 ggatcgttgc tttctggcgc atctggcgtg tcagacatca aggccgcaaa atcattagtg
    19381 aaaacgtaag tcccggtgta atcggggttt ttatcgcctg tcacccgcac attacctgcg
    19441 cagaggaagc aatctggatc gtgcgcaggt aacacctgtt tggctggcgt ttcctgcgcc
    19501 ccctgccagg ggcgcttagc gcggtgcggt gaaaccagaa tccattgccc ggtgagcggg
    19561 ttgtagcggc gatgtggatg atcaacggga ttaaattgcg tcatggtcgt tccttaatcg
    19621 ggatatccct gtggatggcg tgactgccag tgccaggtgt cctgcgccat ttcatcgagt
    19681 gtgcgcgtta cgcgccagtt cagttcgcga tcggctttgc tggcgtccgc ccagtaggcc
    19741 ggaaggtcgc cctcgcgacg cggtgcaaaa tgatagttaa ccggtttgcc gcaggctttg
    19801 ctgaaggcat taaccacgtc cagcacgctg ctgcctacgc cagcgccgag gttgtagatg
    19861 tgtacgcctg gcttgttcgc cagtttttcc atcgccacga cgtgaccgtc cgccagatcc
    19921 attacgtgga tgtaatcgcg tacgccagta ccatcttcgg tcggataatc gttaccaaaa
    19981 atcgccagcg agtcgcgacg gcctacagca acctgggcga tgtatggcat caggttattc
    20041 ggaatgcctt gcggatcttc acccatatcg cccgacggat gcgcgccaac cgggttgaag
    20101 tagcgcagca gggcaatgct ccagtccggc tgggcttttt gcaggtcggt gaggatctgt
    20161 tccaccatca atttgctttt gccataaggg ctttgcggtg tgccggtcgg gaagctttca
    20221 acgtatggaa ttttgggctg atcgccataa acggtggcgg aggagctaaa aataaagttt
    20281 ttgacgttag cggcgcgcat ggcgctaatc aggcgcagag tgccgttgac attgttgtcg
    20341 taatattcca gcggtttttg taccgattcg ccaacggctt tcagcccggc gaagtggatc
    20401 acggtgtcga tagcgtgatc gtgcaggatc tcggtcatca acgcttcgtt acgaatatcg
    20461 ccttcaacaa acgttggatg tttgccgcct aaacgctcga taacaggcag tacgctgcgc
    20521 ttactgttac agaggttatc aagaatgatg acatcatgac cgttttgcag taattgcaca
    20581 caggtatgac ttccaatgta accgctacca ccggtaacca gaactctcat aattcgctcc
    20641 attaggctta tggtatgaaa taaccatagc ataacaaaga tgcgaaaagt gtgacatgga
    20701 ataaattagt ggaatcgttt acacaagaat ttagccgttt tttatgcgcg attaagtgat
    20761 tataaaacag agggtttatg aatgattgcg ctttttatct gaaaaaagac gcggtttcat
    20821 gcctgcatgc gtcgaactgt aggccggaga gggggcttac gccacatccg gcacagtcag
    20881 cattaccaac tcagtttatt tttgtcagcg catagcgata aaggtcaccg tccggcacga
    20941 actcaagacg atgggtaata caggcaggcg catcttcagc gtggtgcgaa acaaacaaca
    21001 attgcgtttc accttcgcta atcagcacat caacaaaacg gcggataagt tggcgattga
    21061 gcggatcaag cccctgtagt ggttcatcga gaataagcaa tgtcggatgt ttcaccagcg
    21121 cgcggacgat cagcgccaga cgctgctgtc cccaggaaag actatggaac ggaacgtcag
    21181 ccgtgcgttt atcaatgccg agaatatcca gccactgctg caccagtttt tgctggcgat
    21241 ccgaaacggc ctgataaatg ccaatcgaat caaaatagcc agaaagaatc acattacgca
    21301 cggtagtgct gacccggtaa tccagatgca aactactgct gacgtaaccg atatgctttt
    21361 tgatatccca gatggtttcg ccgctgccgc gacgtcggcc gaaaagcgtc aaatcgttgc
    21421 tgtaaccttg cggatgatcg ccagtaatca ggcttaataa cgtcgatttt cccgcaccat
    21481 ttggcccgac aatttgccag tgctcgccag gattcacctg ccagctaagg ttattaagaa
    21541 tggggcgatc gttataagaa accacgccat tattcagcac aatgcgcggt tcgttggtgg
    21601 gtaaggcgtg acgtgctgaa ggttcatccg gctccggcag ttgcacacct tcaagctgtt
    21661 cgctatgcgc cagttgcgcg acgagtgctt gctggagcag ttcctcttta gcgcccgttt
    21721 ccgctaacgt acaatccgcc agcacgccag caaactggac aaattccggg atctcatcga
    21781 agcgattgag caccagtacc agagtaatac cggactgatg taacgaggcg agtcgctcag
    21841 ccagctgctg acgtgaggca acatccaggc catcgaacgg ctcatcaaga atcaacaagt
    21901 caggctccga catcagcgcc tgacacagca gggtttttcg cgtctcgcca gtggaaaggt
    21961 atttaaagcg tcggtcgagg agggcggtaa taccgaactg ctgcgccagt tgcatgcaac
    22021 gcggtgcatc cttaacttca tcctgaatga tctcagccgt agtgcgtccg gtgtcatctt
    22081 cgccagggcc gagcatatcg gtgttattcc gctgccattc gtcgctgacg agcttttgca
    22141 attgctcgaa ggagagacga gtgatgtggg aaaactggct ttgccgttca cctttcaaaa
    22201 gcggaagttc ccccgccagc gcacgggcca gcgccgactt tccacttcca ttcgaaccga
    22261 caaacgccca actatcaccc gcgtttaacg ttagctgagg caattgcagc gtttttgtgt
    22321 cgctaagacg aaacgtgcct tgcaaaattt gcaacgatga cattttatat cccattttgt
    22381 gcagcgatta ctgataggga tacgtgtttc ataacaaatt gtcaacacgc ttagcatagc
    22441 gtggcgataa tcacgctgtc ggcattaaag taggccgtga cattctgtcc ttgctgaaga
    22501 gaagtcgctt cattaaccgg cactgtggcg cacagtgttt gcccgtcggg tagcgccatt
    22561 aatacttcgc actgctctgc gccgcgctca atatgactaa taatacccgg taattggttg
    22621 tcggcgtttt gtgcgaccgc ttcgtcctga gtaatgccta cccacggcgc ttttagcaat
    22681 atcaacactt ctttgccttc atccagcccc agacgtgcgc cgctttgtgc ggtaattgcg
    22741 actttcaggc gtgtttttcc gtcagccagt aagacatcaa catgctgttg aacgtcatca
    22801 tgatcgcggg cggtgatggt gccgaaccac tggttacggg cgctggtttg cagtgaaaaa
    22861 cgtgagatcg cggccagcag gctgttcagc ggcagggcgt catcgtcact taacacatca
    22921 aaggcttttt gctggatttg cgccagtaag tcatagagct gaatcagtcg ctgaccatag
    22981 cgggtcagta ctgcgccgcc gccaccttta ccgcctgttg cgcgctcgac cagaatatgc
    23041 tcacttaact gattcatctc gttaatggca tcccaggcgc ttttatagct gataccggca
    23101 tctttcgctc cctggctaat ggaaccggaa agcgcaatgt gttttagtag cgaaatgcgg
    23161 cgcgggtcgg cgaataattt ttgttggagc ttaagggtga gaaggatttc ggcctgcata
    23221 acaatgtcct ggcaaaagtc ttattgtgac ggaaaacgaa cgccacgcaa agctgaccgc
    23281 acaaaagggg agtgcttttc tgtgcttagc ggttagaata gtctcatgac tatatctgga
    23341 gttgaccatg ttagagttat taaaaagtct ggtattcgcc gtaatcatgg tacctgtcgt
    23401 gatggccatc atcctgggtc tgatttacgg tcttggtgaa gtattcaaca tcttttctgg
    23461 tgttggtaaa aaagaccagc ccggacaaaa tcattgattc cctgaatgcc cgcttagtcg
    23521 ggcattttct ttttctcaac ttcctgcttt tcctgccgat attttttctt atctacctca
    23581 caaaggttag taataactgc tgggaaaatt ccgagttagt cgttatattg tcgcctacat
    23641 aacgttacat taaggggtta ccaatggctc gtaaatggtt gaacttgttt gccggggcgg
    23701 cactctcttt cgctgttgct ggcaatgcac tggcagatga agggaaaatc acggtgttcg
    23761 ccgccgcatc actgactaac gcaatgcagg acattgctac gcagtataaa aaagagaaag
    23821 gcgtggatgt ggtttcttct ttcgcttcgt catctactct cgcccgtcag attgaagcgg
    23881 gtgcgcctgc ggatctgttt atttctgccg atcagaaatg gatggattat gcagttgata
    23941 aaaaagcgat cgatacagct acgcgtcaga cactgctcgg caatagcctg gtcgttgtag
    24001 caccgaaagc cagcgagcag aaagatttca tcatcgacag caaaactaac tggacttcgc
    24061 tgctgaatgg cggtcgcctc gcggttggcg atccggaaca tgtccctgct ggcatttatg
    24121 caaaagaagc actgcaaaaa ctgggcgcat gggatacgct ctccccgaaa ctggctccgg
    24181 cggaagatgt tcgtggcgcg ctggcgttgg tcgaacgcaa tgaagcgccg ttgggcattg
    24241 tttacggttc tgacgcagtt gccagcaaag gggtaaaagt ggttgccacc ttcccggaag
    24301 attcacataa aaaagtggaa tatccggttg ctgttgtgga agggcataac aatgcgacag
    24361 tgaaagcatt ttatgattat ctgaagggac cgcaggctgc ggaaatcttt aaacgttacg
    24421 gatttacaac caagtaatga tactgaccga tccagaatgg caggcagttt tattaagcct
    24481 gaaagtttct tccctggctg tgctgtttag cctgccgttt gggatctttt ttgcctggtt
    24541 actggtgcgt tgcacgtttc cgggcaaagc tttgctcgac agcatactgc atctaccgct
    24601 ggtgttaccg cccgtggtcg tcggttactt attattagtt tcgatgggac ggcgcggatt
    24661 tatcggtgaa cgtctgtatg actggtttgg tattaccttc gcctttagct ggcgcggcgc
    24721 ggttctcgct gccgccgtca tgtcgtttcc gctaatggtg cgggcaattc gtctggcgct
    24781 ggaaggggtt gatgtcaaac tggaacaggc cgcaagaaca ctgggggccg ggcgctggcg
    24841 cgttttcttt actatcacgt taccgctgac cttaccggga attattgttg gtacggtact
    24901 ggcttttgct cgttctctcg gtgaatttgg cgcaaccatc acctttgtgt cgaacattcc
    24961 tggtgaaacg cgaaccattc cttctgccat gtataccctg attcagaccc ccggcgggga
    25021 aagtggcgca gcgagactgt gcattatttc tattgcgctg gcgatgatct ccctgttgat
    25081 ttcagaatgg ctggccagaa tcagccgtga acgggcgggg cgctaatcat gctggaactg
    25141 aatttttccc agaccttggg caaccattgc ctgactatta atgaaacgct gcccgccaat
    25201 ggcatcactg ctatctttgg cgtctccggt gccggaaaaa cttcgctgat taacgccatc
    25261 agtggactga cgcgcccgca aaaagggagg attgtcctca atggtcgggt actaaatgat
    25321 gccgaaaaag gtatctgcct gacgccagaa aaacgtcgcg ttggctatgt ttttcaggat
    25381 gcacggctgt tcccgcatta caaagtgcgt ggcaatttgc gctacggcat ggcgaaaagc
    25441 atggtcgatc agttcgataa gctggtggcg cttttaggca ttgaaccgtt gcttgaccgt
    25501 ttaccgggca gcctgtccgg aggcgaaaaa cagcgcgtgg cgattggtcg ggctttgctg
    25561 acggctccgg aattgctgtt gctggatgaa ccgctggcgt cactggatat tccgcgtaaa
    25621 cgcgaactgt tgccttatct gcaacgtctg acgcgggaaa tcaacattcc gatgttgtat
    25681 gtcagccatt cgctggatga gatcctccat ctggcagaca gagtgatggt actggaaaac
    25741 ggtcaggtga aagcctttgg cgcgctggaa gaagtctggg gtagtagcgt gatgaatccg
    25801 tggctgccga aagagcaaca aagtagcatt ctgaaagtga cggtacttga gcatcatccg
    25861 cattacgcga tgactgcgct ggcgctgggc gatcagcatt tgtgggtcaa taagctggac
    25921 gaaccgctgc aagctgcgct acgtatccgc attcaggctt ccgatgtttc tctggtttta
    25981 caaccgccgc agcaaaccag cattcgtaac gtattgcggg caaaagttgt taatagttat
    26041 gacgacaacg gccaggtgga agtggagctg gaagtcggcg gtaaaacgct gtgggcgcgt
    26101 atcagcccgt gggccaggga tgaactggcg atcaaacctg gcctgtggct gtacgcgcaa
    26161 attaaaagtg tgtcgataac cgcctgatta aatcaggtgg ctataaatga actgggcaat
    26221 gctgtcggtg gtgttatcac caatcacaat gttggcgcgc gcttttaccg cgtcatcggc
    26281 gttgcccatc gccacgcctg taccagcggc ttccagcata ctgatatcat taaagttatc
    26341 gccgaatgcc acgacgtttt ccatcgacca accttgcgcc tcaacccatt tcgtcaaacg
    26401 tttacctttg ctgttgccgc cgcgtgcaat atcaacctga tcgtgccagg accattcaca
    26461 ctccagtccc agttcatgtt cgacatgctt accaaaatgc tgcaattgcg gcaggtcatc
    26521 gtgcgtcagg gcgaacttcc atacggcgtt aacttgttgc gccgtttcag ccagagaagc
    26581 gacttgtgtg aaagtcggac gctgttccgg cggcagggtt tgcgcccagt tagacgtgcg
    26641 aatgacatgc ccggtcgggt gctcatagac cattgcatca tcgacataca tcagaccgtg
    26701 aatgtggtgt tcattcagca tctcaatgag ttgcagggct ttattaacgg gcattgggtc
    26761 cgcttccagc acggtttttg catgataatc atacaaatag gtgccattac agcaaatagc
    26821 aggtgtatcc agcgccagcg cctgataaaa aggatgaata gcgacgtgat ggcgacctgt
    26881 gacgatgatt aattgatagc ctgcttcgcg agcgcgggcc agggcttcta tcgatgaagg
    26941 aagcagggtc tttttcgggg tcaataaggt gccgtctaag tcgagagcaa tcacgcgtgt
    27001 ggtcatggcg tattccagat taaggttaag aattttcgtc tgcgcgaatg gtacaccgat
    27061 accactatcg gacaaaattc tgcgttttaa ttcagcattc accgccaaaa gcgactaatt
    27121 ttagctgtta cagtcagttg ctaaatgcaa aggagcattc atgaagcaaa cagtttatat
    27181 cgccagccct gagagccagc aaattcacgt ctggaacctg aatcatgaag gcgcactgac
    27241 gctgacacag gttgtcgatg tgccggggca ggtgcagccg atggtggtca gcccggacaa
    27301 acgttatctc tatgttggtg ttcgccctga gtttcgcgtc ctggcgtatc gtattgcccc
    27361 ggacgatggc gcactgacct ttgccgcaga gtctgcgctg ccgggtagtc cgacgcatat
    27421 ttccaccgat caccaggggc agtttgtctt tgtaggttct tacaatgcgg gtaacgtgag
    27481 cgtaacgcgt ctggaagatg gcctgccagt gggcgtcgtc gatgtggtcg aggggctgga
    27541 cggttgccat tccgccaata tctcaccgga caaccgtacg ctgtgggttc cggcattaaa
    27601 gcaggatcgc atttgcctgt ttacggtcag cgatgatggt catctcgtgg cgcaggaccc
    27661 tgcggaagtg accaccgttg aaggggccgg cccgcgtcat atggtattcc atccaaacga
    27721 acaatatgcg tattgcgtca atgagttaaa cagctcagtg gatgtctggg aactgaaaga
    27781 tccgcacggt aatatcgaat gtgtccagac gctggatatg atgccggaaa acttctccga
    27841 cacccgttgg gcggctgata ttcatatcac cccggatggt cgccatttat acgcctgcga
    27901 ccgtaccgcc agcctgatta ccgttttcag cgtttcggaa gatggcagcg tgttgagtaa
    27961 agaaggcttc cagccaacgg aaacccagcc gcgcggcttc aatgttgatc acagcggcaa
    28021 gtatctgatt gccgccgggc aaaaatctca ccacatctcg gtatacgaaa ttgttggcga
    28081 gcagggacta ctgcatgaaa aaggccgtta tgcggtcggg cagggaccaa tgtgggtggt
    28141 ggttaacgca cactaaccgc tgatttaacc ggcgcagtct ctcctgcgcc ggtgtattaa
    28201 cttatctcct gtaacgcatg tctctggcgt tcgacgatat tggtccacaa attgtcttta
    28261 tcgtcagtcc acaaattaat cagcaaggca aaaaagcgtt cagctgccgg agaaagcacg
    28321 gcatctttgc ggcgaataat ccccaatgtc cgacgtataa cgggttccac cagtgggata
    28381 ccaataatcg aagaataggg cgcatggggc attgccagac caggaagcgc cgaaatcccc
    28441 agtcctgcct ccactagtcc taatgacgtc gaaagatgac gcacttcgta aaaccagtcc
    28501 agcttccagg gcttgtcggc tagctgttgt tctatcaaca atcggttgcc gctggaggaa
    28561 cgtacgccaa tcattttgta acccactaac tcctgccatt ctacaagctg ctttttggcc
    28621 aatggatggt cacgccgaca ggccagcacg aatggctcgt tgaccagtgg ggtaaaatca
    28681 atggatgaat ttgtgacgtt gttcatgttt atgccaaagt ctgattcgtt acacagcacc
    28741 gattccatgc aattattcgt gccttgttcc agaatccgca ctttaatatt gggatacagc
    28801 tcattaaatt taccgattgc cagcggtaaa aaataaaata ctgcggtcgg aatacacgct
    28861 aacgtcacca taccacgatg ataagcattc atatcacgaa tattaaaaag cgtttcatcg
    28921 aatttcttta ttaattcccg cgcctccggg agcaaccttt tccctgcttt tgttaacgta
    28981 accttgcgag ttgtacgctc aaatagttga atatgtaaat cttcctccat cttttttatt
    29041 cttcgcgtta atgcaggttg cgtaatattg agtgatttag cggcattgtt aaatgaactg
    29101 gactctgcca gtatgacaaa tgccttcata cttgataatt catgcttcat tattactccg
    29161 gaaaatggaa gcgacgattt tgggtggctg gccgttaaaa attttaactg catttagcca
    29221 acttaaatta atgaaaaaat gttattaatc gttgagctaa agtcattaga gatgctttgc
    29281 ccttaatgta accatatcgc aataagttat gtttttaaat tgagggcatt attatgaaaa
    29341 aaataccctg cgtgatgatg cgaggtggaa cctcgagggg cgcgttcttg ttagcggaac
    29401 atttacccga agatcaaacg cagcgcgata aaatattgat ggcaattatg ggttccggta
    29461 acgatctgga aattgacggt attggcggcg gtaatccgct gaccagtaaa gtcgccatta
    29521 ttagccgttc cagcgatccg cgtgctgatg tcgattatct gtttgctcaa gttattgtcc
    29581 atgagcaacg tgtcgatacc acgcctaatt gtggcaatat gctgtctggt gttggggcat
    29641 tcgccattga aaatggtttg attgcagcga cttcaccagt tacccgcgta cgtatccgca
    29701 acgtcaatac tggtacgttc atcgaagctg atgtgcaaac gccaaatggt gttgtcgagt
    29761 acgagggtag cgccagaatt gacggcgtac cgggtactgc cgcaccggtt gcgctcactt
    29821 tcctgaatgc cgccggaacc aaaaccggaa aagtcttccc gactgataat cagattgatt
    29881 attttgacga tgtcccggtg acctgtatcg atatggcgat gccagtcgtc attattccgg
    29941 ctgaatatct gggaaaaaca ggttatgaat taccggcgga actggatgcc gacaaagcat
    30001 tattagcacg cattgaatct atccgtctac aagcgggtaa agcaatgggc ttaggtgatg
    30061 tcagtaatat ggttatccct aaacctgtgc ttatttctcc agcgcagaaa ggcggggcaa
    30121 ttaatgtacg ttattttatg ccgcattctt gccatcgcgc gctggcaata accggtgcta
    30181 ttgctatttc cagtagttgt gcattggaag gcaccgtcac ccgacaaatc gtcccttctg
    30241 taggatacgg caatatcaat attgaacacc ccagtggtgc gctcgacgtt catttaagta
    30301 atgaaggtca ggatgccacg acgttacgcg catctgttat tcggacgacc agaaaaatat
    30361 tttccggtga agtttatctt ccctgaaaaa attcgttgtc aggataagga caatcaataa
    30421 aggacttctg tatgagtcat acagaaagaa caggatttta aatgaataag aaatcgttat
    30481 ggaagctaat tctgatatta gcgatcccat gtattattgg ttttatgcca gctccggcag
    30541 gattaagcga actggcgtgg gtgctttttg gtatttacct ggcggccatt gtgggtctgg
    30601 ttatcaagcc tttcccggaa cctgtcgtac tgttaattgc cgttgctgcc tcaatggtgg
    30661 tggtcggtaa cttatccgac ggtgcgttta agaccaccgc cgtattaagc ggttactctt
    30721 caggtactac ctggctggtg ttctcggcgt ttaccttaag cgccgcattt gtgaccaccg
    30781 gtttaggtaa acgtattgcc tatctgctga ttggtaaaat cggtaacacc acgctgggtc
    30841 tgggttacgt tacggtattc ctcgatctgg tactggctcc ggcaacaccg tctaacaccg
    30901 cgcgtgcggg cggcattgtg ttaccgatca tcaacagcgt ggcggtggct ttggggtccg
    30961 aaccggaaaa aagtccgcgt cgtgtcggac attacctgat gatgtccatt tacatggtca
    31021 ccaaaaccac cagctatatg ttctttaccg caatggcggg gaacattctg gcgctgaaaa
    31081 tgatcaacga cattctgcac ctgcaaatta gctggggtgg atgggcgctg gccgccggat
    31141 tgccgggcat cattatgctg ctggtcaccc cgctggtaat ttacaccatg tatccaccag
    31201 aaattaaaaa ggtggataac aaaaccatcg ctaaagcggg ccttgccgaa ctgggaccga
    31261 tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct ggcgctactg ggctggattt
    31321 tcagtaagtc tctgggggtt gatgaatcca ctgtggcaat cgttgttatg gcgaccatgc
    31381 tgctgctggg tatcgttacc tgggaagacg tggttaaaaa taaaggcggc tggaatacct
    31441 taatctggta cggcggtatt atcggattaa gctccttatt atcgaaagtt aaattcttcg
    31501 aatggttagc tgaagtcttt aaaaataacc tggcatttga tggtcacggt aacgttgctt
    31561 tcttcgttat tattttcctc agcattatcg tgcgttattt cttcgcttcc ggtagtgcct
    31621 atatcgttgc tatgttaccg gtatttgcca tgctggcgaa cgtctccggc gcaccgttaa
    31681 tgttaaccgc gctggcactg ttgttctcca actcctatgg cggcatggtt actcactatg
    31741 gcggcgcggc aggtccggtc atctttggcg tgggttataa cgatattaaa tcctggtggt
    31801 tggtcggtgc ggtactgacg atattaacct tcctggtgca tatcaccctc ggcgtgtggt
    31861 ggtggaatat gctgatcggc tggaacatgc tgtaaatatt ctcgtcatac ttcaagttgc
    31921 atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgta
//
LOCUS       DAZGCS010000053        31399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGCS010000053 DAZGCS010000000
VERSION     DAZGCS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.053"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(127..3276)
                     /gene="acrB"
                     /locus_tag="ACHK01_002571"
     CDS             complement(127..3276)
                     /gene="acrB"
                     /locus_tag="ACHK01_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO8097503.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="ACHK01_002572"
     CDS             complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="ACHK01_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO8097504.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKARPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4634..5281
                     /gene="acrR"
                     /locus_tag="ACHK01_002573"
     CDS             4634..5281
                     /gene="acrR"
                     /locus_tag="ACHK01_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO8097505.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5409..8771
                     /gene="mscK"
                     /locus_tag="ACHK01_002574"
     CDS             5409..8771
                     /gene="mscK"
                     /locus_tag="ACHK01_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO8097506.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8832..8930
                     /locus_tag="ACHK01_002575"
                     /pseudo
     CDS             8832..8930
                     /locus_tag="ACHK01_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8983..9144)
                     /gene="rsmS"
                     /locus_tag="ACHK01_002576"
     CDS             complement(8983..9144)
                     /gene="rsmS"
                     /locus_tag="ACHK01_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO8097507.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9158..9685)
                     /gene="priC"
                     /locus_tag="ACHK01_002577"
     CDS             complement(9158..9685)
                     /gene="priC"
                     /locus_tag="ACHK01_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO8097508.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9755..10132
                     /gene="ybaN"
                     /locus_tag="ACHK01_002578"
     CDS             9755..10132
                     /gene="ybaN"
                     /locus_tag="ACHK01_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO8097509.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10285..10836
                     /gene="apt"
                     /locus_tag="ACHK01_002579"
     CDS             10285..10836
                     /gene="apt"
                     /locus_tag="ACHK01_002579"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO8097510.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10965..12896
                     /gene="dnaX"
                     /locus_tag="ACHK01_002580"
     CDS             10965..12896
                     /gene="dnaX"
                     /locus_tag="ACHK01_002580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO8097511.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12229..12293
                     /gene="dnaX"
                     /locus_tag="ACHK01_002580"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12949..13278
                     /gene="ybaB"
                     /locus_tag="ACHK01_002581"
     CDS             12949..13278
                     /gene="ybaB"
                     /locus_tag="ACHK01_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO8097512.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13278..13883
                     /gene="recR"
                     /locus_tag="ACHK01_002582"
     CDS             13278..13883
                     /gene="recR"
                     /locus_tag="ACHK01_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO8097513.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13993..15867
                     /gene="htpG"
                     /locus_tag="ACHK01_002583"
     CDS             13993..15867
                     /gene="htpG"
                     /locus_tag="ACHK01_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO8097514.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16048..16692
                     /gene="adk"
                     /locus_tag="ACHK01_002584"
     CDS             16048..16692
                     /gene="adk"
                     /locus_tag="ACHK01_002584"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO8097515.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16928..17890
                     /gene="hemH"
                     /locus_tag="ACHK01_002585"
     CDS             16928..17890
                     /gene="hemH"
                     /locus_tag="ACHK01_002585"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO8097516.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17887..18846)
                     /gene="aes"
                     /locus_tag="ACHK01_002586"
     CDS             complement(17887..18846)
                     /gene="aes"
                     /locus_tag="ACHK01_002586"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO8097517.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18998..20302
                     /gene="gsk"
                     /locus_tag="ACHK01_002587"
     CDS             18998..20302
                     /gene="gsk"
                     /locus_tag="ACHK01_002587"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO8097518.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20435..22111)
                     /gene="ybaL"
                     /locus_tag="ACHK01_002588"
     CDS             complement(20435..22111)
                     /gene="ybaL"
                     /locus_tag="ACHK01_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO8097519.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGLVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22349..23569)
                     /gene="fsr"
                     /locus_tag="ACHK01_002589"
     CDS             complement(22349..23569)
                     /gene="fsr"
                     /locus_tag="ACHK01_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO8097520.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23787..25439
                     /gene="ushA"
                     /locus_tag="ACHK01_002590"
     CDS             23787..25439
                     /gene="ushA"
                     /locus_tag="ACHK01_002590"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8097521.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25476..25955)
                     /gene="ybaK"
                     /locus_tag="ACHK01_002591"
     CDS             complement(25476..25955)
                     /gene="ybaK"
                     /locus_tag="ACHK01_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO8097522.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26159..26953)
                     /gene="ybaP"
                     /locus_tag="ACHK01_002592"
     CDS             complement(26159..26953)
                     /gene="ybaP"
                     /locus_tag="ACHK01_002592"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO8097523.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27091..27432
                     /gene="ybaQ"
                     /locus_tag="ACHK01_002593"
     CDS             27091..27432
                     /gene="ybaQ"
                     /locus_tag="ACHK01_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8097524.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27647..30151)
                     /gene="copA"
                     /locus_tag="ACHK01_002594"
     CDS             complement(27647..30151)
                     /gene="copA"
                     /locus_tag="ACHK01_002594"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO8097525.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30413..31345
                     /gene="glsA"
                     /locus_tag="ACHK01_002595"
     CDS             30413..31345
                     /gene="glsA"
                     /locus_tag="ACHK01_002595"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO8097526.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
BASE COUNT         7670 a         8347 c         8236 g         7146 t
ORIGIN      
        1 aattttatcc gtggttaata ctggttttcg tatgagatcc tgagttggtg gttcaattac
       61 tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg cgcggcctta gtgattacac
      121 gttgtatcaa tgatgatcga cagtatggct gtgctcgata tcttcattct tgcggctaaa
      181 gcggcggcga accacaacaa agaataccgg aacgaagaag attgccagta ccgttgcggt
      241 caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac cagcaccagt
      301 actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca ggatcggacg
      361 taaacgcatc cgcactgcat caagcgtcgc ttcaatcaga cctttacctt ctttatccat
      421 caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc caatggttgt
      481 gagcaggcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa
      541 cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg accagctctc
      601 gtacagcgcc gccagacaca ggaatacgac aatcaacgaa atcgcgtaca gtgaaggtgc
      661 ctggttgccg gagaggcgtt cctgatagga catccccgtc cagtcatagc caacaccggt
      721 aggcagtttg ctcgccagtt gttccattag ctccattgct tcaccggtac ttttacccgg
      781 tgccgcctgg cctaagattt ccatggatgg caggccattg taacgttcca gacgcggcga
      841 accgtactcc caacgagaag aggagaatgc cgagaacggc accatctgac catcagcagc
      901 acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt ctgacatgac
      961 gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac cgccccatgc
     1021 agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg
     1081 gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta cgctggtcag
     1141 catatcaggg tgcttcgctg cttctgcaag cagctggtta cgcgcctgag tcagtttttc
     1201 gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcag tacccagttc
     1261 cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg agaaagcgcg
     1321 tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat cggcccagtc
     1381 cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt
     1441 aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat gcgttacctc
     1501 attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat
     1561 aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacga acagataggc
     1621 catgccgacc acgatgatca gatacagcac caggtaacgc cccgtactgc gcagaatacc
     1681 gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa accagccgaa
     1741 gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca
     1801 aagagccgga gtcaggatca acgctaccag taccgacagc gccattgctg aaacaatggt
     1861 aatagagaac tgacgataga tagcaccagt agaaccgcca aagaaggcca tcggtacgaa
     1921 taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc ccatcgactt
     1981 acgggtagct tcttttggcg gcaaaccttc ttccgccata acacgctcca cgttttctac
     2041 cacaacgata gcgtcatcca ccaacaagcc gatagcgagc accatcccga acattgttag
     2101 cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca ataccaccgg
     2161 tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat acataaccag
     2221 gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag agattttcac
     2281 gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga acggttccat
     2341 cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac cggtcgccag
     2401 cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt cgtagttctc
     2461 accacccagc tcaatcttcg ccacatcacg cagcagtacg cgggaaccat cttgattcac
     2521 tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag caataataga
     2581 ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgacccg ccgcaacctg
     2641 ggcgttctgc gctttgatgg cggtaatgac atcaaccggc gttagctgga atttgttcag
     2701 ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact gaacatcacc
     2761 cacgcccgac gtacggctga tggcatcttt catattcgcc gctacgtagt cagagatatc
     2821 ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga agctgctgga
     2881 tgatttctca acgctcaccc cttgctgctg aacttcttgc ggcagcaacg gcatcgccag
     2941 ctgcagtttg ttctgcacct gaacctgcgc gatgtccgca tcagtaccag actcaaaggt
     3001 cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca tcaggttatc
     3061 gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc
     3121 agcgccgggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag gatattgcgc
     3181 caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg cgatcaccca
     3241 cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc tgtttaagtt
     3301 aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa
     3361 cttcttgtgc ttttacctgg acaccaggac gcgctttctg cagcccactt attactacgc
     3421 gatcgcctgc tttcagacct tctgtcacca gccacttatc gccaatagcc tggcttgcaa
     3481 cgatcggacg ggtttccact ttgtcatccg cgccaactac cagtacggtg gcatcaccac
     3541 gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta agcccttctt
     3601 ccagacgtgc gcgcacgaac atacccggca gcagagtgtg atccgggttc gggaagatag
     3661 cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat tccagcgtac
     3721 cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct ttgccgttct
     3781 cttgtttcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg ttgctggact
     3841 gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac
     3901 cgttctgtac caatgcgcct tccgtcacgt tcgacttacc aatgcgaccg ctaatcggag
     3961 aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct ttcgccgcag
     4021 ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct tgcttactga
     4081 tgtactgagt accgagcagt ttctgataac gattcaccgt caattgcgcg atattggctg
     4141 cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgtcgcctga taggtcgcag
     4201 gatcaatctg atagagagag acaccagctt cgatgtcgct accttctttg aaattacgct
     4261 tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac
     4321 ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg ccaacggcgg
     4381 gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc
     4441 ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc atatgtaaac
     4501 ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt
     4561 tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct aacgcctgta
     4621 aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc gccaacacat
     4681 cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct cgctgggcga
     4741 gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta aagacaagtc
     4801 ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac tagagcttga
     4861 gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat taattcatgt
     4921 tcttgaatcc acggtgacag aagaacggcg tcgattattg atggagatta tattccacaa
     4981 atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc tctgtctgga
     5041 aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc
     5101 ggatttaatg acgcgtcggg cagcaattat tatgcgcggc tatatttccg gcctgatgga
     5161 aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc
     5221 catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcca ctaacgaata
     5281 accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat tctggttcac
     5341 tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt
     5401 cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt ttttcagcat
     5461 ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg gcgtcatcga
     5521 atggtgatct gccgacaaaa gcggacctgc aggcgcaact tgactcacta aataaacaaa
     5581 aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca ttagccaccc
     5641 tcgataaaat cgatcgcata aaagaagaga cagttcagct acggcaaaaa gtcgctgaag
     5701 cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagcgat gtcgataacg
     5761 acgaagaaac gcgcaaaatt ctgagtacgc tttcgttgcg ccagctggaa actcgcgttg
     5821 cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat aacagccagc
     5881 tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc
     5941 agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact gccttacgtc
     6001 ccagccagaa agtgttaatg caggctcagc aggcgttgct gaatgcggag attgaccagc
     6061 agcgtaaaag cctggaaggg aacaccgtct tgcaggatac cttgcaaaag caacgtgatt
     6121 acgtgacggc gaacagcgcc cgtctggagc accagttgca gctgttgcaa gaagcggtaa
     6181 acagcaagcg cctgacttta actgaaaaaa cggcgcagga agccgtctcc ccggatgaag
     6241 ccgcgcgtat tcaggctaat ccgctggtga aacaggaact ggaaattaac cagcagttaa
     6301 gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa aacattaaag
     6361 tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag cagattgccg
     6421 tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa acgctgccct
     6481 cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg
     6541 aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac aaactggaag
     6601 aaggtcacac caacgaagtc aacagcgaag ttcacgatgc gttattgcaa gtggttgata
     6661 tgcgtcgcga attgctggat caactcaaca aacagttggg taaccagctg atgatggcca
     6721 ttaacctgca aatcaaccag cagcagttaa tgagcgtatc gaaaaacctg aaatccatcc
     6781 tgactcagca aatcttttgg gtgaacagta accgtccaat ggactgggac tggatcaaag
     6841 cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aatcacggtg aactgggaaa
     6901 aagcctggcc tgccgttttt atcgctttcc tcgccggttt gccgctgctg ttgattgccg
     6961 ggctgatcca ctggcgtctg ggctggctga aagcgtatca acagaaactg gcttccgctg
     7021 tgggttccct gcgtaacgat agccagctca acacaccaaa agcgatcctt atcgacctga
     7081 tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg cctgattctg ttgaccatgc
     7141 agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa actggcgatt ttctggctgg
     7201 tgtttggcct gtgctggaag gtactggaga aaaatggcgt tgccgtgcgt cacttcggca
     7261 tgccggagca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt ctcgcgttgc
     7321 tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg atggatgatg
     7381 tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg gtatggccaa
     7441 tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc accattaccg
     7501 tgctgtcgat tatcccgatt gcattgatgg tgctgactgc cacgggttat ttctacacca
     7561 cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct ggtgatcatc tggaacttgc
     7621 tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc tggcgtcgtg
     7681 cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag
     7741 aacccaccat tgcactggaa caggttaacc agcagacgct gcgtattacc atgttgctga
     7801 tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc accgtgttca
     7861 gctatctcga cagcatcacg ctctggcatt acaacggcac tgaagctggc gctgcggtgg
     7921 tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg gtggcctggg
     7981 cgttgattcg caacctgcct ggtttactgg aagtgctggt gctctcgcga ctgaatatgc
     8041 gtcagggcgc gtcgtatgcc attactacca tccttaacta catcattatt gccgttggtg
     8101 cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg ctggccgcag
     8161 cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc gtctccggtt
     8221 tgatcattct gttcgaacgt ccggtgcgta ttggcgatac ggtaaccatt ggtagcttct
     8281 cggggacggt aagtaagatc cgtattcgtg cgacaacgat taccgatttc gatcgcaaag
     8341 aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg
     8401 acactactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat ctggaaaaag
     8461 tgcgtaaagt gttactgaag gcggcgactg agcacccaag ggtgatgcac gaaccaatgc
     8521 cggaagtctt ctttacggcg tttggtgcca gcacgttgga tcatgagctg cgtctgtatg
     8581 tgcgtgaact gcgtgaccgt agtcgtactg tcgatgagct gaaccgtact atcgatcagc
     8641 tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat ctgcacaacg
     8701 agaagggcga tgaggtgacg gaagtcaaac gcgactacaa aggcgatgac ccgacgccag
     8761 cggtagggta ataaccaaag ggggcgacat ttagttgccc cgagattact gacaaagtgc
     8821 gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg cctacgaaac cgcaagaatt
     8881 caatgtattg caggagcggt gtaggcctga taagcgtagc gcatcaggca gttttgcgtt
     8941 tgcccacaat cttaggggac atttagcggc cccatttatt tctcactttt ccgcctcatc
     9001 atcgcgcgtt aatttctttt cataatcgcg ttttacaata tccagcgcgc gcagcacggt
     9061 gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacagcca gtttgacatc
     9121 gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaag cgcgctagcc
     9181 tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca taggcttcca
     9241 cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg cgggctctgc
     9301 gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata cgtttacgct
     9361 gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag gcggaggctt
     9421 cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc acttgcggca
     9481 gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc tcatcgagac
     9541 aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct gtcaaaacgg gcacttagcg
     9601 tggcaaattg cgctaccggg gcacaacgct gacgcagcgt agcgagctgt ccttccagtt
     9661 tttccaggag cagggcggtt ttcacgataa gacctcaatg aaaatgataa ttgttatgct
     9721 aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt taatcatcat
     9781 tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat taccgacgac
     9841 gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct ttcacgcctg
     9901 gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac atcatgcgat
     9961 gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg ccatttctct
    10021 gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg cctgtttgct
    10081 tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact gaagcacaac
    10141 aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga gcacaggcgc
    10201 aggcggtcaa aggttaaaca actgttattt ttgatacgtt taaaacgcgc cgtgagtacc
    10261 accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga gtatctcaaa
    10321 aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg cgatgtcacc
    10381 agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt tgagcgttac
    10441 aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt gtttggcgct
    10501 ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg caaactgccg
    10561 cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct ggagatccac
    10621 gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct ggcaaccggc
    10681 ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt ggctgacgct
    10741 gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa acagggcatt
    10801 accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt gtgctgccca
    10861 cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc tgtgttagca
    10921 ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt tatcaggtct
    10981 tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa catgtgctga
    11041 ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt ttttccggta
    11101 cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta aactgcgaaa
    11161 ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc gagcaggggc
    11221 gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa gatacccgcg
    11281 acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt tatctgatcg
    11341 acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc cttgaagagc
    11401 cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg ccggtgacga
    11461 ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa attcgccatc
    11521 aacttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg ttgcaattgc
    11581 tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac caggcgattg
    11641 ccagcggtga cggccaggtt tcaacccagg cagtcagtgc gatgctgggt acgcttgacg
    11701 acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag cgcgtaatgg
    11761 cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg gtggaaatgc
    11821 tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt ggcaacgaca
    11881 tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg acggatattc
    11941 agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg ccggaccgcc
    12001 gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt atgcctctgc
    12061 ctgagccaga agtgccacgc cagtcctttg cgcccgtcgc gccaacggca gtaatgacgc
    12121 caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc
    12181 cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa
    12241 ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg
    12301 cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc
    12361 tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggctaccact ccagtgatgc
    12421 agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa
    12481 cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacgcg tgggcggcac
    12541 aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggccttaaat gcctggaaag
    12601 aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc
    12661 gcggtgcaca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca acggttgaac
    12721 tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga
    12781 tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga
    12841 ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt
    12901 aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa
    12961 ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg
    13021 caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct ggtaaaagtg
    13081 accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac
    13141 gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt
    13201 gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc
    13261 tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc
    13321 gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc
    13381 gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg
    13441 gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc
    13501 cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca
    13561 ttgagcagac ggggcagttt tcaggccgtt attttgtgtt gatggggcac ctgtcaccgc
    13621 tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt ctggcagagg
    13681 aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta
    13741 actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg
    13801 taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac tcccttgccg
    13861 ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct cgcttgaaat
    13921 tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg
    13981 aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt
    14041 ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc
    14101 tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg
    14161 tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg
    14221 accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact
    14281 atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac
    14341 agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa
    14401 gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg
    14461 gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc
    14521 accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc
    14581 atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa
    14641 gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct gtggactcgt
    14701 aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat cgcccacgac
    14761 tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc
    14821 ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc
    14881 ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt catgccgaac
    14941 tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt
    15001 gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg
    15061 ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaca aataccagac cttctggcaa
    15121 cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc
    15181 gccaaactgc tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg
    15241 gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac
    15301 agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa
    15361 gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac
    15421 ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa
    15481 gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgtgtgaaa
    15541 gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca
    15601 gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg
    15661 gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg
    15721 gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt agaactgctg
    15781 ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt
    15841 cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca
    15901 tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg tttcagcctc
    15961 aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaat
    16021 tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg
    16081 gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact
    16141 ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac
    16201 attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc
    16261 attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg
    16321 caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta
    16381 ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt
    16441 gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa
    16501 gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac
    16561 catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc
    16621 aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga tctggaaaaa
    16681 atcctcggct aattcgaaag cgcgcacgga cagtcccctc gccccctcgg ggagagggtt
    16741 agggtgaggg gaacaggccc gcacaagcaa acttatcagc aatctcaggc cggatattca
    16801 ttcggccttt tacaaaaacc tcaatctgcg ctattccttt ttctgatttg acctctcaca
    16861 gcaattagtt cttcttcctc acttttccgc tacaattatc aacaagttga atcgataaga
    16921 ggtggtaatg cgtcagacta aaaccggtat cctgctggca aacctgggta cgcccgatgc
    16981 ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca gacgcgtggt
    17041 tgatacctca cggttgttat ggtggccgtt gctgcgcggc gtgattttgc cgctgcgctc
    17101 gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa gacggctcgc cgctgatggt
    17161 ttacagccgc cagcaacagc aggcgctggc acaacgttta ccggagacgc ccgtagcgct
    17221 gggcatgagc tacggctcgc catcactgga aagcgccgtc gatgaactcc tggcagagca
    17281 tgtagatcat attgtggtgc tgccgcttta tccgcaattc tcctgttcta cggtcggtgc
    17341 ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg ggatatcgtt
    17401 tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca gcgtacgcgc
    17461 ttcttttgcc aaacatggcg aaccggatct gctgctgctc tcttatcatg gcattcccca
    17521 gcgttatgca gatgaaggcg atgattaccc gcaacgttgc cgcacaacga ctcgcgaact
    17581 ggcttccgca ctggggatgg caccggaaaa agtgatgatg acctttcagt cgcgctttgg
    17641 ccgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg gagaaaaagg
    17701 cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg gattgtctgg agacgctgga
    17761 agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc ggcgggaaaa aatatgaata
    17821 tattccagcg cttaatgcca cgccggaaca tatcgaaatg atggctaatc ttgttgccgc
    17881 gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct cgtcggcggt
    17941 tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga gtttgaactc
    18001 acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat ccagcagcgg
    18061 atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat cattattaaa
    18121 cagacagtaa tacggtgact cgcggtccgc gtcgttgctt aaatacgcct cttcatacat
    18181 ctgcaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac gagtcacgga
    18241 atcccgtaat ccgtaaagcc cataccacag caaaacaccc gcaattttac cgcaatcgat
    18301 ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg cggaatcacc
    18361 ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt ggaaataaca
    18421 acaagcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg aaagggggta
    18481 atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga tgcgatcgtg
    18541 ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg tcgccgggct
    18601 atctggctgc ggacaaaaga gacgtgtttc cacctgccca tattttgttg gaaccatgta
    18661 agctctggtt gccatttctg gagcgcccgc attccagaat cggcgctcaa gcgtgtaata
    18721 ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg gctgaagagt
    18781 attcacaacg gtcttcattt cagcagaaat aaggtccaga acaggtagtt tgttttccgg
    18841 cttcatacaa aaactccttt caaattacgt cattgtaagg aaccactgcc atgaaaatgc
    18901 gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca gattgtgtta
    18961 acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa
    19021 atccaaacat tacttccctg taaatgcacg cgatccgctg cttcagcaat tccagccaga
    19081 aaacgaaacc agcgccgcct gggtcgtagg tattgaccag acgctggtcg atattgaagc
    19141 gaaagtggat gatgaattca ttgagcgtta tggattaagc gccgggcatt cactggtgat
    19201 tgaggatgac gtagccgaag cgctttatca ggagctaaaa cagaaaaacc tgattaccca
    19261 tcagtttgcg ggtggcacca ttggtaacac catgcacaac tactcggtgc tcgcggacga
    19321 ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa attggcagtt atgcctatcg
    19381 ttacctgtgt aacacttcca gccgtaccga tcttaactat ctacaaggcg tggatggccc
    19441 gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg ctatcagtcc
    19501 aggccacatg aaccagctgc gggctgaaag cattccggaa gatgtgattg ccggagcctc
    19561 ggcactggtt ctcacctctt atctggtgcg ttgcaaaccg ggtgaaccca tgccggaagc
    19621 aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc tgacgctggg
    19681 caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca aagaccacgt
    19741 ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa gcgatccgtt
    19801 gttggcatct gacaaggcgc tggactgggt agatctggtg ctgtgcaccg ccgggccaat
    19861 cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgct
    19921 gctgccgggc gctatagcgg aattcaacca gtatgagttt agccgcgcca tgcgccacaa
    19981 ggattgccag aatccgctgc gtgtatattc gcacattgcg ccgtacatgg gcgggccgga
    20041 aaaaatcatg aacactaatg gagcggggga tggcgcattg gcagcgttgc tgcatgacat
    20101 taccgccaac agctaccatc gtagcaacgt accaaactcc agcaaacata aattcacctg
    20161 gttaacttat tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt
    20221 actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag acagcctgga
    20281 agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag gcgttcacgc
    20341 cgcatctggc atttggctct cgatgcccga tgcgacgctg gcgcgtctta tcatgcctac
    20401 atatttttca tattttacat ccggcaacca ccgtttaccc cgtcaccact tcacctgccg
    20461 gtggcgtttc cagcagttcc agcatagtac gggcaatttc acgttcaccc atcactacct
    20521 gattcgcgcc acgttcggtg atatacgtca cttcatcgtc ataatgggcg cgggcgataa
    20581 tctcaatatc cgggtttttc gcgcgggcag acgccacaat ctcacccgct tcataaccgt
    20641 tggggatcgt caggatcagc cattttgcac attccagatg cgccagttgc ataatttctt
    20701 cgttcgctgc attgcccaat acagcgcgga ccccacgctc tcgcagctca tcaacacggg
    20761 ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc tcccccagca
    20821 ggctgcctac acgaccgtaa cccaccaata gcgcatggtt gcaaatatcc actgggatct
    20881 gtttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca
    20941 gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg atcgcccctg
    21001 ccagtaccag gttttgtccg gcctgcggca gtaaattcaa tgccattccc agtcccgcca
    21061 ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaat gccgtacgtt
    21121 gggagtgacc aaacagtcgc accaggaaaa atgcggctaa cgacttacca aacagaataa
    21181 tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga tcaaacaaca
    21241 tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc gtatcgtggg
    21301 cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac gcaccgagtg
    21361 caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc agcgccagca
    21421 ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct gcgtgccata atccacggca
    21481 ccagacggcg acctaccagc atcataatgg cgataaatgc gatcactttg ccgatggtga
    21541 tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc attcctgcca
    21601 ctgcgggcag caacaccagc gtcagaacca ttaccaggtc ttccacaatc aaccaaccga
    21661 tggcgatttg cccacgctga ctgtcaatta attgccgttc ttcaagtgcg cgcagtaaca
    21721 ccacggtact ggcggtggaa agacataaac cgaacacgag accggtcatt aacgaccagc
    21781 ccagcacggc agagagcgcc atacccagca gcgtcgccac ggctatctgg gcgatcgcac
    21841 cgggaatggc gatggccttt accgccatca aatccttcag cgaaaagtgc aaaccgacgc
    21901 caaacatcaa cagaatgacg cccagttcag ccagttccgg cgcaagcttg gtatcggcaa
    21961 caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc accagaggag
    22021 aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg ccgccaacaa
    22081 tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc
    22141 catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt cattgatgat
    22201 gttcatgaat aattattgaa ttttgcagaa aaatggaatt agctgcaaaa aaagcacgga
    22261 tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag
    22321 ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata
    22381 tggtcaacat ccccaatagt ggcaggaaag cacagatttt atagactaac tcgatgctgg
    22441 tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatgcca aaagcaaaac
    22501 cgaaaaagag tccagaaacc ataccgatac gtcccggaag tagctcctga gcgtagacca
    22561 gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa acccccgtcc
    22621 agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg atagagcccc
    22681 aaatcacata tttccgccca attttatccc ctacaggccc gccgatcacc gtacctgccg
    22741 caacggcaaa caggaaggca aacagatgaa gctgggcatt ctggatagat aatccgaatt
    22801 tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa tatttcgaga
    22861 aaatgaggat taacagaatg ctgactgcca gtaccacttt gttgcgcggc agtggattga
    22921 ttatcgtcgc tttggatttt cctttattca ttcggtgctg tgccgagtac caacggctaa
    22981 tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca acgttgcctt
    23041 tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg ccaaagttgc
    23101 cgccgacctg aaagatagat tgcgccaggc catgccgccc gccggaagcc atacgggcta
    23161 cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc gccgccagca
    23221 gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag accacttaag gtaaagcaca
    23281 tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg accactggtt
    23341 gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc ataaatgtca
    23401 gagaaaattc tgactgaagc agcggataaa tcgccagaat cagcgattgg atcatgtcgt
    23461 tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg gccttggtcg
    23521 ttgacgcagc cgcgcccgcc acaggctggg gttgttcact cattgccata ggaaagtcac
    23581 tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg actaacatac
    23641 ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca gaatttctaa tctggatgca
    23701 gatttatctt caccggacgc agacttgtct atgatgtcgc gtcatactat ttttcaacac
    23761 gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg gcgttagcgc
    23821 tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtatgagcag gataaaacct
    23881 acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc aatgaatatg
    23941 gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg
    24001 ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc gtgcccgagt
    24061 ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc tatgacgcga
    24121 tggcgatcgg taatcatgaa tttgataatc cgctcaccgt attacgccag caggaaaagt
    24181 gggccaagtt cccgttgctt tccgcgaata tctaccagaa aagtactggc gagcgcctgt
    24241 ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat tgccgtcatt gggctgacaa
    24301 ccgatgacac agcaaaaatt ggtaacccgg aatacttcac tgatgtcgaa tttcgtaagc
    24361 ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag ccagacatta
    24421 ttatcgcggc gacccatatg gggcattacg ataatggtga gcacggctct aacgcaccgg
    24481 gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc gtcggtggtc
    24541 actcgcaaga tccggtctgc atggcggcag aaaacaaaaa acaggtcgat tacgtgccgg
    24601 gtacgccatg caagccagat caacaaaacg gcatctggat tgtgcaggcg catgagtggg
    24661 gcaaatacgt gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa atggttaact
    24721 accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc
    24781 gcgtgcttta cactcctgaa atcgctgaaa accagcaaat gatctcgctg ttatcaccgt
    24841 tccagaacaa aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat ggtcgtctgg
    24901 aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg attctggcag
    24961 cccaaatgga tcgcactggt gccgactttg cggtgatgag cggaggcgga attcgtgatt
    25021 ctatcgaagc aggcgatatc agctataaaa acgtgctgaa agtgcagcca ttcggcaatg
    25081 tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc gccgtcgcgc
    25141 agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt gtggcgaaag
    25201 acggcaaact gaacgacctt aaaatcaaag gcgaaccggt cgatccggcg aaaacttacc
    25261 gtatggcgac attaaacttc aatgccaccg gcggtgatgg atatccgcgc cttgataaca
    25321 aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg tatatccaga
    25381 aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat
    25441 ccgaaagtgc cggatgtttg catccggcac aatgcttaat cgcggcgggc gatatcagca
    25501 aatttggcat cgaggatctt tgccagatcg cctgccgcca gttcgatatc cagtccgcgc
    25561 ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc
    25621 agacgttttt tctgccccag tgggctaatc cccccaacca ggtatcccgt cgaacgctgc
    25681 gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc tactttttta
    25741 agatccagtt gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc
    25801 actgccacca gcagcgtttt gtagacctga tccggattca aacctaattt tttgacgact
    25861 tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg aaacgaaatc
    25921 ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa
    25981 aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt
    26041 attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa agtgacggca
    26101 taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg ccattttttt
    26161 agcgcaacat ttgcggcaaa ttcccttctc catacaggtg tagtgcaccg accgcgacca
    26221 catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca
    26281 tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatatca ttattttgcg
    26341 gcggtgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt
    26401 gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc aacatggcaa
    26461 tctggttttc agccccttcc agttcaatca cgggtttatg ttgttgcttc gccgcctgca
    26521 ataactggta atcgataccg tattctgccc gcagccccag tttttgtgcc tgcgtcgcct
    26581 gaagaaccat cgcgatttgc cacagcggtt gagtagaaaa gagcgacgga gaaatgccca
    26641 tctcctggct aatgtgctgc agattttgga gttgctcctc gttaatgcgc tcttccagcg
    26701 cctcgcaagt aggcaaatta gcaaaaggcg tatcgctggt ggaaacatcc gcctcgacga
    26761 tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga gccatatcgt
    26821 ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccggga agggtgatat
    26881 cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt
    26941 acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg tgagggatgg
    27001 cgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatgt tcttgcatcg
    27061 ctattcacag ggaagcaaca gttaaaaacc atgaaacagg caacaagaaa accgacgaca
    27121 cccggagata ttcttctcta tgaatatctg gaaccgctcg atttgaaaat caatgagtta
    27181 gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa
    27241 ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt cgattttagg
    27301 ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg cacccaggaa
    27361 gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa
    27421 aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc gttcacgccg
    27481 catccggcat ccgagcacca ttgcctgatg cgacgcttac ccgtcttatc aggcctacaa
    27541 acctgtgcct gaaccgtagg ccggataagg cgtttacgcc gcatccggca gtcatgcgtc
    27601 gatgccaaat gcgccgccct aaagcagcgc atccgcaatg acgtacttat tccttcggtt
    27661 taaaccgcag caaccggttg gcgttactca ctacggtaat cgacgagagc gccattgccg
    27721 ctccggcaac taccgggtta agcagtgttc cagtaaacgg ccacaaaata ccggcggcga
    27781 ccggaatacc gatgctgttg tagataaagg caccgagcag gttctgcttc atgttgcgca
    27841 gcgttgcgcg ggaaatcgcg agcgcatccg caacgcccat caggctatgg cgcatcaggg
    27901 taatcgccgc ggtttcaatg gcaacatcac tgccgccacc catcgcaatg ccgacatccg
    27961 cctgggccag cgctggcgcg tcgttaatgc cgtcgcccac cattgccacc tgacgtcctt
    28021 cactttgcag gcgtttaatc gcttcggctt taccgtccgg cagcaccccg gcgatcactt
    28081 catcaatccc ggcttctttg gcgatcgcat tggcagtggt tgggttatcc ccggtcaaca
    28141 tcaccagacg atatcccgct ttatgcaggc gttgcagcgc cgccacgcta tcactacgca
    28201 atggatcgcg tactgccagc agggctaccg ctttcccgtc aaccgccagc agcacaggcg
    28261 ttgccccttg cgatgcctga gcagtaatct ccgcttcgat agctttggta ccaacctgtt
    28321 gctcatttaa cagcgcctga ttgcccagca ataacgcatg accttcagct tcaccgctca
    28381 cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata tcacctgctt
    28441 tatcgaggat cgcccgtgcc agcggatggc tggaaccttg ctccagtgcc gccgccagac
    28501 gcaatgcctg cgcttcatca acatcagcaa acgttttcac tgcgacaacc tgcggcttcc
    28561 cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc gagtgtactg gcgcgttgca
    28621 gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc ccgcccgacg ccggaaataa
    28681 tcgacatcgg cgtcgccagc cccagcgcac acggacaggc gataatcagt accgtggtgg
    28741 caatcaccag ggtataaaca atttgcggtg ccggaccaaa gaagtaccag attgccgcac
    28801 tgaccagcgc aatcaccacc accacaggta caaatacggc tgagatttta tccgccagct
    28861 gaccgatttc tggcttgctg ctctgggcct ggcgcaccat gcgaatgatc cgtgacagcg
    28921 tggtatggct gccaaccgca ctggcacgaa acagcacgct accgtcctgt accactgtcc
    28981 cggcatggac gctttcgcct tcgccttttt gctgcgggat tggttcgccc gtcagcatcg
    29041 cttcatccag ccatgcttcg ccctgggtaa tctcgccatc taccggcacg cgatcgccgg
    29101 tcgtcaggcg cagcaacata cctggctgca cttccgccag aggcacgctt ttttcacctt
    29161 cgtcagtaac caggcgcgcc gtcggtgggg ttaaatcgag taatttctcc agcgccttag
    29221 aagaacgctg acgtgcgcgt gcttccagca tatggccgag attgatcaga ccgataatca
    29281 tcgcgctggc ttcgtaataa agatgtcgcg cttccatcgg gaaccactgc ggccacaggt
    29341 tgacgctcat cgaatagagc cacgccacgc cagtacccag cgccaccagc gtatccatcg
    29401 tcgctgcacc gttcagcagg cttttccatg cactgcggta aaaatggccg ccggcgaaaa
    29461 ccatcaccgc cagggttatc aggccgataa ccaaccacag gctgcggttg tcagcggtga
    29521 ccatcatgtt atcgccaatc atcccccaga ccatcaccgg gatacccacc gccagtgcga
    29581 caattgcctg ccagcggaag cgcttcatcg tagcaacggc ggtttcctgc tggcgctcac
    29641 ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc cgctttttcc accgcctgca
    29701 ctaaatcttg tggggaggca ctgcccatca ccagcgcagt acgctccgcc aggtttaccc
    29761 gtgcctgagt gacgcccggt acgctttgca gcgcattttg tacgcgggtg acacagctgg
    29821 cgcagctcat gccgctcagc agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg
    29881 gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat tgatgactcc gccagcggtt
    29941 tagcctttgg gtggcttaca gatgcgtcat aacccgcttg tttgatggtt tcgatcagct
    30001 gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat agacacatcc gcttgttcca
    30061 catccgggcg ctgttcgaga ctttctttca cgcgtttaac gcagtgaccg caggacaggc
    30121 cgtccagggt caggtcgata gtttgtgaca taaaacactc ctttaagaca gttttgactg
    30181 gctgtgataa aggttaaacc ttccagcaag gggaaggtca agaaattaat aaaccaggcg
    30241 agtaaaagtc cgtaaagatt aaaaaattgg ctcaatttgc atcaggatta gacatttatc
    30301 tctttgtttt cctgtagtta agttgcgggt gctaagttaa atcaagatgc ctgaaaatcg
    30361 gcaccggggt gaggaattac ctcccgcatc tataaaaagg agttaacaaa agatgttaga
    30421 tgcaaacaaa ttacagcagg cagtggatca ggcttacacc caatttcact cacttaacgg
    30481 cggacaaaat gccgactaca ttccctttct ggcgaatgta cctagtcaac tggcagcagt
    30541 ggctattgtg acctgcgatg gcaacatcta tcgcgcgggt gatagtgatt accgctttgc
    30601 gctggaatcc atctcgaaag tctgtacgtt agcccttgcg ttagaagatg tcggcccgca
    30661 ggcggtacag gacaaaattg gcgctgaccc gaccggattg ccctttaact cagttatcgc
    30721 cttagagttg catggcggca aaccactgtc gccactggta aatgctggcg ccattgccac
    30781 caccagcctg attaacgctg aaaatgttga acaacgctgg cagcgaattt tacatatcca
    30841 acagcaactg gctggtgagc aggtagcgct ctctgacgaa gtcaaccagt cggaacaaac
    30901 aaccaacttc cataaccggg ccattgcctg gctgctgtac tccgccggat atctctattg
    30961 tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc tccacgctcc tcaatactgt
    31021 tgaactggca acacttggcg cgacgctggc ggcggggggt gtgaatccgc tgacgcatga
    31081 acgcgttctt caggccgaca acgtgccgta cattctggcc gaaatgatga tggaagggct
    31141 gtatggtcgc tccggtgact gggcgtatcg cgttggttta ccgggcaaaa gcggtgtagg
    31201 tggcggtatt ctggcggtcg tccctggcgt gatgggaatt gccgcgttct caccaccgct
    31261 ggacgaagaa ggcaacagtg ttcgcggtca aaaaatggtg gcatcggtcg ctaatcaact
    31321 cggctataac gtgtttaagg gctgatcatg atgaacacgg aaggtaataa cggtaacaaa
    31381 cccctcggtc tatggaatg
//
LOCUS       DAZGCS010000054        31314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGCS010000054 DAZGCS010000000
VERSION     DAZGCS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.054"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..273
                     /locus_tag="ACHK01_002596"
     CDS             <1..273
                     /locus_tag="ACHK01_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8097527.1"
                     /translation="IRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQM
                     GHADAQMVFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            complement(461..1480)
                     /gene="rspB"
                     /locus_tag="ACHK01_002597"
     CDS             complement(461..1480)
                     /gene="rspB"
                     /locus_tag="ACHK01_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO8097528.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1492..2706)
                     /gene="rspA"
                     /locus_tag="ACHK01_002598"
     CDS             complement(1492..2706)
                     /gene="rspA"
                     /locus_tag="ACHK01_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO8097529.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2912..3238)
                     /gene="ynfA"
                     /locus_tag="ACHK01_002599"
     CDS             complement(2912..3238)
                     /gene="ynfA"
                     /locus_tag="ACHK01_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO8097530.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3373..3714
                     /gene="ynfB"
                     /locus_tag="ACHK01_002600"
     CDS             3373..3714
                     /gene="ynfB"
                     /locus_tag="ACHK01_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO8097531.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3749..4309
                     /gene="speG"
                     /locus_tag="ACHK01_002601"
     CDS             3749..4309
                     /gene="speG"
                     /locus_tag="ACHK01_002601"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO8097532.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(4312..5022)
                     /gene="ynfC"
                     /locus_tag="ACHK01_002602"
     CDS             complement(4312..5022)
                     /gene="ynfC"
                     /locus_tag="ACHK01_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO8097533.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VNPAVERVYTIKNTIDYY"
     gene            5130..5435
                     /gene="ynfD"
                     /locus_tag="ACHK01_002603"
     CDS             5130..5435
                     /gene="ynfD"
                     /locus_tag="ACHK01_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO8097534.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5634..8060
                     /gene="ynfE"
                     /locus_tag="ACHK01_002604"
     CDS             5634..8060
                     /gene="ynfE"
                     /locus_tag="ACHK01_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO8097535.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            8121..10544
                     /gene="ynfF"
                     /locus_tag="ACHK01_002605"
     CDS             8121..10544
                     /gene="ynfF"
                     /locus_tag="ACHK01_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO8097536.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GQDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10555..11172
                     /gene="ynfG"
                     /locus_tag="ACHK01_002606"
     CDS             10555..11172
                     /gene="ynfG"
                     /locus_tag="ACHK01_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO8097537.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            11174..12028
                     /gene="ynfH"
                     /locus_tag="ACHK01_002607"
     CDS             11174..12028
                     /gene="ynfH"
                     /locus_tag="ACHK01_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO8097538.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            12071..12685
                     /gene="dmsD"
                     /locus_tag="ACHK01_002608"
     CDS             12071..12685
                     /gene="dmsD"
                     /locus_tag="ACHK01_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO8097539.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12844..14136
                     /gene="clcB"
                     /locus_tag="ACHK01_002609"
     CDS             12844..14136
                     /gene="clcB"
                     /locus_tag="ACHK01_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO8097540.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(14089..14784)
                     /gene="bioD"
                     /locus_tag="ACHK01_002610"
     CDS             complement(14089..14784)
                     /gene="bioD"
                     /locus_tag="ACHK01_002610"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8097541.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14909..16129)
                     /gene="mlc"
                     /locus_tag="ACHK01_002611"
                     /gene_synonym="dgsA"
     CDS             complement(14909..16129)
                     /gene="mlc"
                     /locus_tag="ACHK01_002611"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO8097542.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLLGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(16264..17157)
                     /gene="ynfL"
                     /locus_tag="ACHK01_002612"
     CDS             complement(16264..17157)
                     /gene="ynfL"
                     /locus_tag="ACHK01_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8097543.1"
                     /translation="MNIELRHLRYFVAIAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYEDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            17264..18517
                     /gene="ynfM"
                     /locus_tag="ACHK01_002613"
     CDS             17264..18517
                     /gene="ynfM"
                     /locus_tag="ACHK01_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097544.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(18566..18685)
                     /locus_tag="ACHK01_002614"
     CDS             complement(18566..18685)
                     /locus_tag="ACHK01_002614"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO8097545.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            18941..19249
                     /gene="asr"
                     /locus_tag="ACHK01_002615"
     CDS             18941..19249
                     /gene="asr"
                     /locus_tag="ACHK01_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO8097546.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETANTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            19342..19425
                     /locus_tag="ACHK01_002616"
     CDS             19342..19425
                     /locus_tag="ACHK01_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097547.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            19525..20346
                     /gene="ydgD"
                     /locus_tag="ACHK01_002617"
     CDS             19525..20346
                     /gene="ydgD"
                     /locus_tag="ACHK01_002617"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO8097548.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20385..20714)
                     /gene="mdtI"
                     /locus_tag="ACHK01_002618"
     CDS             complement(20385..20714)
                     /gene="mdtI"
                     /locus_tag="ACHK01_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO8097549.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20701..21066)
                     /gene="mdtJ"
                     /locus_tag="ACHK01_002619"
     CDS             complement(20701..21066)
                     /gene="mdtJ"
                     /locus_tag="ACHK01_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO8097550.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVMLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            21478..21621
                     /locus_tag="ACHK01_002620"
                     /pseudo
     CDS             21478..21621
                     /locus_tag="ACHK01_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(22341..22622)
                     /locus_tag="ACHK01_002621"
     CDS             complement(22341..22622)
                     /locus_tag="ACHK01_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097551.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(22636..24297)
                     /locus_tag="ACHK01_002622"
     CDS             complement(22636..24297)
                     /locus_tag="ACHK01_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8097552.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(24281..24637)
                     /locus_tag="ACHK01_002623"
     CDS             complement(24281..24637)
                     /locus_tag="ACHK01_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO8097553.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(24760..24942)
                     /locus_tag="ACHK01_002624"
     CDS             complement(24760..24942)
                     /locus_tag="ACHK01_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097554.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(24926..25366)
                     /locus_tag="ACHK01_002625"
     CDS             complement(24926..25366)
                     /locus_tag="ACHK01_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8097555.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(25366..25662)
                     /locus_tag="ACHK01_002626"
     CDS             complement(25366..25662)
                     /locus_tag="ACHK01_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8097556.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(25659..25997)
                     /locus_tag="ACHK01_002627"
     CDS             complement(25659..25997)
                     /locus_tag="ACHK01_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8097557.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(25994..27205)
                     /locus_tag="ACHK01_002628"
     CDS             complement(25994..27205)
                     /locus_tag="ACHK01_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8097558.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(27207..27779)
                     /locus_tag="ACHK01_002629"
     CDS             complement(27207..27779)
                     /locus_tag="ACHK01_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO8097559.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            complement(27819..28976)
                     /locus_tag="ACHK01_002630"
     CDS             complement(27819..28976)
                     /locus_tag="ACHK01_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8097560.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(29268..29492)
                     /locus_tag="ACHK01_002631"
     CDS             complement(29268..29492)
                     /locus_tag="ACHK01_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097561.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNINDYRAFLETGALIEKDRHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            complement(29618..29890)
                     /locus_tag="ACHK01_002632"
     CDS             complement(29618..29890)
                     /locus_tag="ACHK01_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8097562.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMMLVETDKERHHITMRRL
                     ECSRKAQRPPEPPDNFGDLRNAVNRTCAEMGIDGAGDEIRRNYQDS"
     gene            complement(29901..30311)
                     /locus_tag="ACHK01_002633"
     CDS             complement(29901..30311)
                     /locus_tag="ACHK01_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8097563.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADQLAGHEKGNVISVSGTMQISQWTGQNGETRQGYQVIADSVISA
                     KTVRPGGSRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(30321..30626)
                     /locus_tag="ACHK01_002634"
     CDS             complement(30321..30626)
                     /locus_tag="ACHK01_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097564.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(30623..30874)
                     /locus_tag="ACHK01_002635"
     CDS             complement(30623..30874)
                     /locus_tag="ACHK01_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097565.1"
                     /translation="MTKMRRDRTQQKYKALDMTELSLKVAIKVIDRHTREGYAKENPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(31222..>31314)
                     /locus_tag="ACHK01_002636"
     CDS             complement(31222..>31314)
                     /locus_tag="ACHK01_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097566.1"
                     /translation="KVYGCEYITRRVKGLTQTNVTTTEDCDAFL"
BASE COUNT         7499 a         8189 c         7556 g         8070 t
ORIGIN      
        1 ataaggcaga tgtgggacgc agcggtaaag cgtgccggaa tccgccatcg caaatcgtat
       61 cagtcgagac acacttatgc ctgctggtcg ttaacagcag gagctaaccc ggcattcatt
      121 gcaaaccaga tgggccatgc agatgcgcaa atggtgtttc aggtgtacgg gaaatggatg
      181 tcagaaaata ataatgcgca ggtaacgctg ttaaatacac agttaagcga gtttgcccca
      241 accatgcccc ataacgaagc gatgaaaagt taatttttta tttatcaatt agttagattg
      301 aatgactctt gaaatccata aattcaagcg cagtgcccag ccatcccgat actgctgctt
      361 tcaccaaatc cttagtgctt ctttcgtgtt tttctattgt cataatggtt atctctaaaa
      421 aagaggtaag atgcgtacta cttactcgcc gttattggta ttattcagaa aaagtgagta
      481 agactttgca gcaatgtttt tgatcctgtt caaataaact aatggcatca gcaacatgct
      541 ggaaatcaaa cgtatgggta attaattttt ctggtttaat taacccttta cttaaccagt
      601 cgataacgac cgggaattta tttgcattta agcgtgaaga aaaaatagag agttcttttc
      661 cggtaattcc ttgctgaatc acttcagacg gttcactgga gaagcccatc aatacaatac
      721 gtgccgctgg agaagccagc gttacggcct ctttcaggat agaaggatga caagccgcat
      781 cgataattaa tgtcggcttg atgccttttt cagtgaaaat ctcgccaagc ggtgtctggc
      841 tgttattaat cgcccagtca gccccgctct ctttcgcttt ttccagtcgt tcatcaatgc
      901 gatcggcaac aatcacattt ttaacgttat agacgccttt taatacctga acgatcgtca
      961 ggccgattgg accggcacca taaaccagaa cggtatcatt ttcagtcggt tgaccatgtc
     1021 cggttacgtt agccgcaatg gtaaaaggtt cgatcattac cgcatattga tcggccactg
     1081 cttcaggaat tttccacgca ttttttgccg gaaccacggc atattcactg aaaccaccgt
     1141 cagcgtgcac acctaataca gccagtgtcg tacaaacgtt cggtttacct atagagcacg
     1201 gatagcaatg cccacagctg accaccggat cgacagcaac acgttcaccg actctggcgc
     1261 tttccacgcc ttcacccact gcatcaatga cgccaaagaa ttcatgacca atgacgcgcg
     1321 gatatttcgc aaaaggatta tgcccacgat aaatatggct atctgaacca caaattccgg
     1381 caagtttcac ttttactcgc acttcacccg ctgacggggt gggtatttca cgttcgacaa
     1441 tcgccagttg attcggtttt tcaattaata tgcttttcat tatcttactc cttaccagtt
     1501 ccacagcgtg ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt
     1561 cgccgccagc ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata
     1621 gccgttatcg aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat
     1681 gtattcctgg acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca
     1741 gactggtgac aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa
     1801 atcggcaatc cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata
     1861 atcgatgagt tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc
     1921 cactgcgatg ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc
     1981 aggtgtcggg tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc
     2041 cgcttcaata ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa
     2101 cttgttacgt accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca
     2161 cagctgctct tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt
     2221 cgacatgccg taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa
     2281 tcccagctcc tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt
     2341 atggcaataa accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat
     2401 gttggccgct ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat
     2461 cgtaaccgga ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat
     2521 acggtgcgca tcgcgaccaa taagctgcgg acaaaggtga tcctgcaaat aagaggccac
     2581 ggaaagctca cgtccattga gagtggcatc cccaaggccc gtaataccgt cctcagtggt
     2641 gatttttaat gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat
     2701 cttcatgttc gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcta
     2761 ctaccataca agtatgagga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt
     2821 tgcgctgtga atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct
     2881 cgtatcttat tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga
     2941 tcaacatgcc gcaaagcgca atcaacgcac ccgtccagtc ataaagagtc agtttcacgc
     3001 catccacaac gcgcagccac atcaacgccg tgcagacata aacgccacca taagccgcgt
     3061 aaacacgccc actcgccgca ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa
     3121 tccccgccgg aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa
     3181 agcatccaat aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct
     3241 ttgtcactta ttgacatcat gtatagttat agggccacat aatatcacca atataaacac
     3301 cctcctgata cgttttgcgt tcgcagtgga tggtgtagaa tcacctttaa tcattcatac
     3361 agggaatgaa ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc ttttctgcta
     3421 ccttgcgcac tggcattgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag
     3481 tctggcgaca gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac
     3541 acgcgcaatc tgcgccagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac
     3601 gccgcttttg ataaccgcga taaatgtgag caaagcgcca acatcaatgc ctactgggag
     3661 ccaaatactt tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt
     3721 attgatttaa ggaatgtaag gacacgttat gccaagcgcc cacagtgtta agctacgccc
     3781 gctggagcgt gaagatttac gctatgtaca tcaactcgac aataacgcca gtgtgatgcg
     3841 ttactggttt gaggaaccct acgaagcctt tgttgaactc tctgatctgt atgataagca
     3901 tattcacgat cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct
     3961 ggtggagctg gtggaaatta accatgttca tcgccgcgca gaatttcaga taattatctc
     4021 cccggagtat caggggaaag gtctggcaac ccgtgccgca aaattagcaa tggactatgg
     4081 ctttaccgtt ctcaatctct ataagctgta tctgatcgtt gataaagaga atgaaaaagc
     4141 gattcatatt taccgcaagc ttggcttttc ggttgaaggt gaattgatgc acgagttctt
     4201 tattaatggt caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc
     4261 agagcacaaa acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata
     4321 atcgatcgta tttttgatgg tgtaaacccg ttcgacggcg gggtttactc cttcatcaac
     4381 aatgatcagc tgacagtcca ccggattggc gtgactgtca tattcacagc tctgttttac
     4441 attaccaact cgttgattat tcagtaaagt aactgctgta taatctaatt ttttgatcgg
     4501 gtccgttgat ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc
     4561 cagcggatac ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt
     4621 aatcacgaag ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag
     4681 ctggcatttt ccctgtaaac gtactctttt ctccagcgtc tcagcatcac ggtaataatt
     4741 ggcgtccagt accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc
     4801 aaaacagcct tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc
     4861 cattaatgtc tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt
     4921 agagaaactg gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt
     4981 gaggaatatc gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta
     5041 gagatgattc tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa
     5101 agaagttatc ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc
     5161 tgctcgccct cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga
     5221 gcgatatatc acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta
     5281 ttgtacccaa tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgtgctaatg
     5341 atacgcataa aattctctac acccgcacca ccagcggtaa cgtctctgct cccgcgcagt
     5401 ctacccagga tggcgcgcct gccgaaccgc agtaacacat tatcgactga acgccggata
     5461 cgacacatcc ggcgatttga acatacaaca taatcccacc ttattactca tacccttcta
     5521 ttgatatgga ttaataattc ttaacccaaa atgggtagac tccctctatt gttagcgcgc
     5581 taaatattca atatataaac ttttatataa cgataaagaa cagggagtga gttatgtcca
     5641 aaaatgaacg aatggtgggc atcagccgca gaacactcgt taaatctacc gcgataggtt
     5701 ctctggcgct ggctgcaggc ggtttttctt tgccgtttac cctgcgcagt gcagcagcag
     5761 cggtacaaca ggcccgcgaa aaagtggtct ggggtgcctg ttccgtcaac tgtggtagcc
     5821 gctgtgcact tcgtctacat gttaaagata atgaagtgac ctgggtggaa actgacaata
     5881 ccggcagcga tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc
     5941 gtcggcgtat taatcatccc gatcgcttga attatccaat gaaacgcgtg ggcaaacgcg
     6001 gcgaaggcaa attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc
     6061 tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata tattcagtac tcttcgggga
     6121 ttgttggcgg caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga
     6181 actgctacgg cggttcactc aatcagtatg gctcctacag caccgcgcaa atttcctgtg
     6241 ccatgcccta cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca
     6301 agctggtcgt gatgtttggt aacaacccgg cagaaacccg catgagcggc ggtggcatca
     6361 cttatcttct tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt atcgatccgc
     6421 gatataccga tacggctgct ggtcgtgaag atgaatggct tcctattcgc ccgggcaccg
     6481 atgccgcgct ggttgcgggt attgcctggg tattgattaa cgaaaatctc gttgatcaac
     6541 cttttctcga taaatactgc gtcggctatg acgaaaaaac cttacccgca gatgcaccaa
     6601 aaaatggtca ctataaagcc tatattcttg gtgaaggtga tgataacaca gcgaaaacgc
     6661 cgcagtgggc ttcgcaaatt accggtatcc cggtggaccg tatcatcaaa ctggcgcgtg
     6721 aaattggcac agcaaaaccc gcttatatct gccagggctg ggggccgcaa cgccaggcaa
     6781 acggcgagct gactgcacgc gctattgcta tgttgcctat tttgacgggc aatgtcggca
     6841 tcagcggcgg aaatagtggc gcgcgagaat cgacctatac cattaccata gaacgcctgc
     6901 cggtgctgga taatccggtc aaaacgtcaa tctcctgctt cagctggacg gatgctatcg
     6961 atcatggtcc gcaaatgacg gcaatccgcg acggcgtccg cggcaaagat aaactggatg
     7021 tgcccattaa gttcatctgg aactacgcgg gaaataccct ggttaatcag cattctgaca
     7081 tcaacaaaac gcatgaaatt ctgcaagacg aatcgaaatg cgaaatgatt gtggtcatcg
     7141 aaaactttat gacctcatcg gcaaaatatg ccgacattct gctgccagac ctgatgaccg
     7201 ttgagcagga agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc
     7261 tccagccagt caccagcgaa aaattcgaac gtaaaccgat ttactggatc ctgagtgaag
     7321 tcgcgaaacg tcttggacct gacgtctatc aaaagttcac agaaggtcgc acgcaggaac
     7381 aatggttaca acatctgtac gccaaaatgc ttgccaaaga tccagcgtta ccgtcttacg
     7441 acgaactgaa aaaaatgggt atctataagc gtaaagatcc caatggtcat tttgtcgcct
     7501 acaaagcatt tcgtgacgac cccgaggcaa atccacttaa aacgccttcc ggtaagattg
     7561 aaatttattc cagcaggctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag
     7621 tgataagccc attgccagtt tatgcctcaa cctttgaagg ctgggactcc cctgaacgta
     7681 gtaccttccc actgcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg
     7741 gcaacattga tgtcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg
     7801 cgcagaaacg tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag
     7861 ttcggctacc agccaaagtg acaccacgca ttctccctgg ggttagcgct atgggccagg
     7921 gagcctggca cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata
     7981 cgctaaccac tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg
     8041 tcgagatcga aaaaatataa cccactacaa ccataaattc tggcatgaca ttttgtttga
     8101 aaagcaataa gtgagtaatg atgaaaatcc ataccacaga ggcgctcatg aaggctgaaa
     8161 tcagccgtag aagtctgatg aaaacctccg cacttggcag tcttgcgctg gcaagcagtg
     8221 ctttcactct gccattttcc caaatggtcc gggcggcaga ggctccggta gaagagaaag
     8281 cggtctggag ttcctgcacc gttaactgcg ggagccgctg tctgttacgt ttgcatgtga
     8341 aagatgacac cgtgtactgg gtggagtctg atacgacagg tgacgacgtc tacggtaatc
     8401 atcaggttcg cgcgtgttta cgcgggcgct ctatccgccg acggatgaat catcctgata
     8461 ggttgaaata tcccatgaag cgcgtcggca agcgcggtga aggtaaattt gaacggataa
     8521 gttgggacga agccctggat accatcagtg ataatcttcg gcggatccta aaagattacg
     8581 gcaatgaggc tgtacatgtc ctgtacggaa caggcgtaga tggcggaaac atcaccaact
     8641 caaacgtccc gtaccgtctg atgaactctt gcggtggttt tctcagtcgc tatggcagct
     8701 acagtaccgc acagatcagt gccgcaatga gttatatgtt cggtgccaat gatggcaaca
     8761 gcccggatga tatcgccaat acgaaactgg tcgttatgtt cggaaataac ccggcagaaa
     8821 cgcggatgag cggcggtggt gtcacttact acgtcgagca agcccgcgaa cgttcaaacg
     8881 cacgcatgat cgtcatcgat ccacgttata acgacacagc tgccgggcgt gaagatgaat
     8941 ggctgcccat tcgcccaggc accgatggcg cactggcctg tgcgattgcc tgggtactga
     9001 ttactgaaaa catggtcgat cagccatttc tcgacaaata ttgtgttggt tacgatgaaa
     9061 aaacgctgcc cgccaacgca ccacgtaacg cgcattataa agcctatatt ctgggcgaag
     9121 ggcctgacgg catagctaaa acgccggaat gggcagcaaa aatcaccagc atcccggcag
     9181 aaaaaattat ccagttggca cgagagatcg gttcagcaaa acctgcttat atttgtcagg
     9241 gttgggggcc acaacgacat tccaacggcg agcaaacgtc ccgcgctatt gccatgcttt
     9301 ccgttctcac cggtaacgtc ggcataaacg gcggcaactc aggtgtacgc gaaggtagct
     9361 gggatctggg ggtagaatgg ttcccgatgc ttgagaaccc tgttaaaacg cagatttccg
     9421 tctttacatg gacagatgcc atcgaccatg gtaaggaaat gaccgcgacc cgtgatggtg
     9481 ttcgtggaaa agaaaaactg gatgtcccca tcaagttttt atggtgctac gccagtaaca
     9541 cattgatcaa tcaacatggc gacatcaatc acacccatga ggtgcttcag gatgacagca
     9601 agtgcgagat gattgttggc attgaccact tcatgacggc ctcggctaag tattgcgata
     9661 tcctgttgcc cgacctgatg ccaacagagc aagaagacct tatctctcat gaatctgcag
     9721 ggaatatggg ctatgtgatc ctcgcccaac ccgcaacctc tgcaaaattt gaacgcaaac
     9781 ccatctactg gatgctgagt gaagtcgcca aacgcttagg acaagacgtt tatcaaacct
     9841 ttactgaagg tcgcagtcag catgaatgga tcaaatatct ccatgcgaaa acgaaggaac
     9901 gtaaccctga gatgcccgac tacgaggaga tgaaaacgac cgggatcttt aagaaaaaat
     9961 gcccggaaga acactacgtc gctttccgcg cattccgtga agatccccag gcaaacccgt
    10021 tgaaaacacc ttcggggaaa atcgaaattt attctgaacg actggcgaag attgcggata
    10081 cctgggaatt gaaaaaagat gaaattattc atccccttcc agcttatacg ccaggttttg
    10141 atggctggga cgatcccctg cggaaaacct atccactgca gttaacgggc ttccactaca
    10201 aggcgcgtac ccactccagc tacggcaata ttgatgtgtt acagcaggcc tgcccacaag
    10261 aggtgtggat caaccccatt gatgctcagg ctcgcggtat ccgtcatggc gataccgtgc
    10321 gggtatttaa caataatgga gaaatgctga ttgccgcaaa agtgactccg cgtattctgc
    10381 ctggcgttac cgccatcggg caaggtgcgt ggcttaaagc ggatatgttt ggtgaccggg
    10441 tcgatcacgg cggcagtatc aatattctga cctctcaccg cccttcaccg ctggcaaagg
    10501 gaaacccgtc gcacagcaat cttgtccaga tcgaaaaggt ttaaagagta gcccatgacc
    10561 acacaatatg gattttttat cgattccagc cgctgtactg gctgtaaaac ctgcgaactg
    10621 gcgtgcaaag attttaaaga tcttggcccg gaagtcagtt tccgccgtat ttatgaatat
    10681 gctggcggcg actggcagga agataatggc gtctggcacc agaacgtgtt cgcttattat
    10741 ctctccatct cctgtaacca ttgcgatgac ccagcatgta caaaagtctg ccccagcggc
    10801 gcaatgcata agcgtgaaga tggctttgtg gtggttgatg aagatgtttg tatcggctgc
    10861 cgctactgcc acatggcttg tccgtacggc gcaccacagt acaatgctga aaaagggcac
    10921 atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc catatgcgtc
    10981 gaatcctgcc cgctgcgtgc acttgagttc ggtccaattg aagagctgcg ccagaagcac
    11041 ggcactctgg ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc caatatcgtt
    11101 atcaaaccta acgccaacag ccgcccgacc ggcgatacca ctggttatct ggctaatccg
    11161 gaggaagtgt aaaatgggaa atggatggca tgaatggcca ctggttatct ttaccgtatt
    11221 gggtcaatgc gtagtaggcg cgctgattgt tagcggaatt ggctggttcg ccgccaaaaa
    11281 tgacgcagac cgtcagcgta ttgtccgtgg catgtttttc ctctggctgt tgatgggcat
    11341 cgggtttatt gcctctgtca tgcacctggg atcgccactg cgagcgttta actcgctgaa
    11401 tcgaatcgga gcttctggtc tgagtaacga gattgctgcc ggttccatat tttttgccgt
    11461 tggcggacta tggtggctgg tggcggtcat cggtaaaatg ccgcaagcgt tggggaaact
    11521 ctggttactt gtcagcatgg cgctcggcgt cattttcgtc tggatgatga cctgcgtgta
    11581 tcaaatcgac accgtgccaa cctggcataa cggttacact acgctggctt tcttcctgac
    11641 ggtattgttg agcggtccaa tacttgctgc cgcaattctg cgggcagctc gcgttacatt
    11701 taataccaca ccatttgcca tcattagcgt tctggcgctg atcgcttgtg ccggggtgat
    11761 tgtgctgcaa ggtttgtctc tggcgtgtat tcactcatcc gtacaacaag ccagcgcact
    11821 ggtaccagat tacgcctcct tacaggtctg gcgtgtggta ttgttatgcg caggtctagg
    11881 atgctgggtt tgtccgctca ttcgtcgccg tgaacctcac gttgccggac ttattctggg
    11941 gttaatcctc attcttggcg gtgagatgat cgggcgtgtg ctcttttatg gcctgcacat
    12001 gacggtagga atggctattg caggttaaca aattggtgcg gggcaacccg cacatttcag
    12061 gatgttagga atgacccatt tttcacagca agataatttt tctgtcgcgg cgcgcgtgtt
    12121 gggcgcgctg ttttattacg ctccagagag tgcggaagcc gcacctctgg ttgcggtact
    12181 caccagtgac ggctgggaaa ctcagtggcc tctaccagaa gcgtcattag cgccgctggt
    12241 gacggctttt cagacacagt gtgaagagac tcacgcccag gcctggcagc gtttgtttgt
    12301 cggcccgtgg gcactgccgt ctccgccgtg ggggtcggtc tggctggatc gcgaatctgt
    12361 gctgtttggc gattcaacat tggcacttcg tcagtggatg cgcgagaaag gcattcagtt
    12421 tgaaatgaag caaaacgaac cggaagatca ttttggttca ctgctgttga tggctgcgtg
    12481 gttggcggag aatggtcgcc agacagaatg cgaagaattg ctggcatggc acctttttcc
    12541 gtggtcaaca cgttttcttg atgtttttat cgaaaaagca gaacaccctt tctaccgtgc
    12601 actgggtgaa ctggctcgct taacgctggc gcaatggcaa tcacaactgt tgattcctgt
    12661 cgcggttaaa ccgctgtttc gataggatca ccgtaatatt gccggatggt gacgattacg
    12721 cgcaatccgg caataatagg ttacagtgtc acgttttttt atctcttaaa gcacgcactg
    12781 cttttgcggc tggcctcttt tgtcgcaaaa tagtcgcccg tgtttcattg cccatttctg
    12841 ctcatgcatc atctacacat ctatccggat ctgcgcacta tgttccgccg tctgcttatc
    12901 gcaacagtcg tcggtattct cgcggccttt gccgttgccg ggtttcgtca tgcgatgctg
    12961 ctactggagt ggttgttcct caataatgac tccggcagtc tggtcaatgc agcgacaaac
    13021 ctttccccct ggcgacggtt gctaactccg gcgctcggcg gactggcggc gggtttgttg
    13081 ctgatgggct ggcagaaatt tacccaacaa cgccctcatg cgccgaccga ttacatggaa
    13141 gcgttgcaaa ccgatggaca gttcgattac gcagcaagcc tggttaaatc gcttgcctcg
    13201 ctgctggtag taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat tcttttagct
    13261 gcccttgccg cctcctgttt tgcccaacgt tttacgccac gccaggagtg gaaattatgg
    13321 atcgcctgtg gggccgcggc gggaatggct gcggcctatc gtgccccgct tgctggcagt
    13381 ttatttatag ccgaagtgct gtttggcact atgatgttgg cctctctcgg cccggtgatt
    13441 atttccgccg tcgtggcatt gctggttagc aatctgatta atcatagcga cgcgttactc
    13501 tacaacgtac aactctcagt gacggttcag gctcgtgact atgcgctgat tatcagtaca
    13561 ggtgtgctgg caggtctgtg cggaccactg ttgttaacgt taatgaacgc ctgtcatcgt
    13621 ggatttgtga gtctcaaact tgcgccgccc tggcaactgg cactaggcgg gttgatcgtg
    13681 ggtctgcttt ccctgttcac acctgcagtg tggggcaacg gctatagcac cgtacaatcc
    13741 tttttaaccg ccccaccact gttaatgatc attgccggga tcttcctttg taaactgtgt
    13801 gccgtgctgg cgagtagcgg ttccggcgca cccggtgggg tctttacacc gacgctattt
    13861 atcggtcttg ccattggcat gttgtatggt cgtagcctgg gattatggtt ccctgatggc
    13921 gaagaaatta cacttttact cggattgacc gggatggcga cactgttggc ggcgaccacg
    13981 cacgcgccga ttatgtcgac gttgatgata tgtgaaatga ccggggagta tcagctactc
    14041 cccggtttat tgattgcctg cgtaattgcg tcggtaattt cgcggacgtt acaccgtgac
    14101 tctatctacc gccagcacac tgcgcagcat agctaagcgg atgtattgcc ccagttcacg
    14161 ctgttcggcg cgcggcagat aaggcagttc accaatgagc ggtgccggaa gttttttacc
    14221 cagcacatca atgatttccg cataatgcgc cagtcctggg ttgattcggt tagccaccca
    14281 gccaatgagc ggcagcccgt cgttggcgat cgcctgagct gttagcagtg catggttaat
    14341 gcaaccttcc tgaataccga caaccatcaa caccggcagt tgttcctgca ctacccattc
    14401 agagagtgga cgcaaatcat tcatcagact gcgccagccg ccagtccctt ctaccacgac
    14461 atgatcgact ttttcggtca ggtttgccag gccgtttgaa atgagggtgt aattgattgg
    14521 gcaactgtgc gccacgctac tttcttcttc gcttaacgcg ataggattaa ctgcttcata
    14581 aggcagttcg atggttgaaa cactctgcaa caccagggca tctttattac gcagcccttc
    14641 gggtgtctct ttgctcccct tcgctacggg tttatatccc gcaaccgttt ttccctggga
    14701 ggctaacgct tgtagcaatg cgcgggaaac caccgttttc cctacagaag tgtctgtacc
    14761 ggtaataaag aaacgcttca gcatcactaa ctccaccgtt atgcttcaca aatataaacc
    14821 aggaaaataa ttaaccttga aagtctaagt tatgctttcc tggcccaaat tgagatagcg
    14881 caaattttgg tagaacagtt aaaaaatgtt aaccctgcaa cagacgaatc aacaaagaac
    14941 cgttatacat cgcgtctttt accagtgcag cccctgccat cgtgccctgg ttagaaaact
    15001 gagtactctc aacgctgatg tgctgactat acgcaggaag ggcctgctga cggatgctgt
    15061 ctgagatgac cgggaagagg atatctgccg ctttacttaa cggtgagcca atcagtattt
    15121 tttgtgggtt aaataaattc accatgatgg caagaatgcg ccctacatgc gcgcccaccc
    15181 cggtaatgat gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac aatgagtcca
    15241 cggttaacgg ttgtccatgt aacatcgagc tcatggattg attaagacgc agctgtgcca
    15301 gctcaagaat actgtccaca ctggcgatgg tttcgaggca gccgtgattc ccgcaataac
    15361 agcgtttccc atacgggtcg acctgtgtgt ggcctatttc cacgagacta ctgctgcctg
    15421 cgtgtagcag atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg ataaccacct
    15481 gaatcacatc gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc gtccatgcgc
    15541 tgatatcatg ctgaatataa accggtacgc cggtatgctg ctccagcgcc tcgccgagcg
    15601 gcatctcttt tacatcctcg tagaacggca tgcgatgtac aataccattt tccgtatcaa
    15661 taattcccag caaggttatg gcaatcgaag ttagacgctc aagttttttc tggtggcgaa
    15721 taaaaaactg atcgatatgg gaaatgatac gatccagcaa tggcgagtca tcttttaacg
    15781 ccagttcctg cgactcttcc accaccagtt tgctgctcag atcgcgcaga gcaaggaaaa
    15841 tctccccgcg actaatgcgc agagaaagat agtgccaggc ttcagtttca accaccagcc
    15901 ccaccgccgg acggccacgg ttccccgctt ctttgatttc cagctcttgc accaggtgtg
    15961 cttcgagcat ctcacggaca attttagtga tactggcagg agccagctgc gccagacggg
    16021 aaagatcgat acgcgagact ggaccaagct gatcaatcag gcgataaacc gcgcccgcgt
    16081 tggtctgctt tatttgatca atgtgcccag gctggttttc agcaaccacc gcatactccc
    16141 tatattttcg cgctccgaaa taatctgtag gctatggtga agcacttcaa tacgtgtcgt
    16201 caaattttta cttaggcatg tgattaacag cacatttttc gggctttttc gctgaaattt
    16261 ccctcacctg agagcattca gcagatgaat acgaaagttt cgcgcagctg gactttgttc
    16321 atgatgtttc ggccagacca accacatttc agaaaccgca tcctcttctg caatcggcac
    16381 ccagcgcatt tcgttgagct gaacacgttt aaatgacgca ggcaaaattg aaacacccag
    16441 accggcggaa accagaccga tgatggtcat tgcctcgccc acctcctgag tgatgacggg
    16501 cgtcaaatgg taacgtcgca tcagcccgag aatatcttca tacagccctg tcccgacgtg
    16561 cggatcaaaa aagacgaagg gttctttcgc cagttcagcc agcgttacat tcgggttatt
    16621 tgccagggga tgatcgtgcg ggatcatcgc cataagcggt tcatggacga tgactgcgtg
    16681 ttcaagcgtc tccggtaacg ctgtgttacg cagcaatccc atatccagcg ttccttcaat
    16741 gagcggagcg atttgctcac gagtgttcat ttcgcgggtt tgtaaatgga catcgggata
    16801 atcacggcga aacagcgata acgtatcgga caccgcccga ataaaaggag cggacgaagt
    16861 aaaaccaatg cgcaactccc ccgcttcccc ctgatgcagt ctttcagcgc gagcggcagc
    16921 gtcatctacc atagacagga tttgccgact atctgcaaga aactgttttc ctgctgccgt
    16981 cagcaataca ctgcgattag ttcgtgccag cagtcgggca ccaatttgtt gctccagtgc
    17041 ctgaatctgc tgacttagcg gtggttgcga aatattcagg cgggcagcgg cgcgcccgaa
    17101 atgcagctct tccgcaatag caacaaagta acgcagatga cgaagttcaa tattcatatt
    17161 taaaacatct tatttgagat tattaatata ttagacagaa caattcgatt ttcctaacct
    17221 atgtataagc ctgatccaca ggcatattta gcaaggattt caagtgagcc gtactacaac
    17281 tgttgatggc gctccggcaa gcgacactga caagcaaagc atttctcagc caaatcaatt
    17341 tattaaacgc ggtacgccgc aatttatgcg cgtcaccctg gcgctgttct ctgccggact
    17401 ggcaacattt gcacttctct attgtgtgca gcctatcctt ccggtgcttt cgcaggagtt
    17461 tggcttaacg cccgcgaaca gtagtatttc actgtccatt tccacggcga tgttggctat
    17521 tggtttgctg tttactggcc ccctatccga tgccattggt cgcaaaccag tgatggtcac
    17581 ggcgctactg ttggcctcca tttgtacgtt actttcgaca atgatgacca gctggcacgg
    17641 cattttgatt atgcgcgcct tgattgggct ttcgttaagt ggcgtggcag ctgttggcat
    17701 gacttatctt agcgaggaaa tccaccccag tttcgtggcc ttttcgatgg ggttgtatat
    17761 cagcggcaac tcaattggcg gcatgagcgg acgcttaatt agtggtgtct tcacggactt
    17821 tttcaactgg cgaattgctc tggcggcaat cggttgtttc gcgctggcct cggcgttgat
    17881 gttctggaaa atccttccgg aatcacgcca ttttcgcccg acttcgctgc gccctaagac
    17941 gttatttatc aactttcgtc tgcactggcg tgaccgggga ttaccgttat tgttcgcaga
    18001 aggctttttg ctgatggggt cgttcgtcac gctgtttaat tacatcggct atcggttgat
    18061 gctttccccc tggcatgtca gtcaggccgt ggttggctta ttatcgctgg cttatttgac
    18121 cggtacatgg agctcaccca aagccggaac catgaccacc cgctacgggc gcggtccggt
    18181 gatgttgttt tcgacggggg ttatgctgtt tggtttactg atgaccttat tcagctcgct
    18241 gtggctgatc tttgccggaa tgttactctt ctcagcagga ttcttcgctg cccactcagt
    18301 tgccagcagc tggattggcc cccgcgcaaa acgcgctaaa ggccaggcct cctcgctgta
    18361 tctgttcagt tactatctgg ggtcgagtat tgccgggacg ctgggtggtg ttttctggca
    18421 taactatggc tggaacggcg tcggcgcatt tattgctctg atgctggtca ttgctctgct
    18481 ggtcgggacg cgtttgcatc gtcgtctgca cgcctgaaaa ataagcccgg actgcggtaa
    18541 ctacccgtcc ggacttattg cttgctcaaa ccaacgttaa tagccatcct aaaatagacg
    18601 aagcgccagc caattcccgc agcgcgtcta gcgtcatcag gattataagt acccaaataa
    18661 acggattcat tttgctgtgt gtcatttatt actgatgcgc agttattcta ccgctttgta
    18721 agtagtaaaa tagttaaccc gatcaagact actattattg gtagctaaat ttcccttaag
    18781 tcacaatacg ttattatcaa cgctgtaatt tattcagcgt ttgtacatat cgttacacgc
    18841 tgaaaccaac cactcacgga agtctgccat tcccagggat atagttattt caacggcccc
    18901 gcagtggggt taaatgaaaa aacaaattga gggtatgaca atgaaaaaag tattagctct
    18961 ggttgttgcc gctgctatgg gtctgtcttc tgccgccttt gctgcagaga ctgcgaacac
    19021 acctgctccg actgcgacga ccaccaaagc agcgccggcg aaaactacac atcataaaaa
    19081 acagcataaa gcagcacctg cccagaaagc gcaggcggct aaaaagcatc ataaaaatac
    19141 gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac acgccgggaa
    19201 acacagccat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag ttttcaacag
    19261 taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta acgtttagca
    19321 ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat tcttatcatc
    19381 cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta gttatctgat
    19441 tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc ttttaataat
    19501 cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt gggtgcaatt
    19561 agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc ggcaaacgat
    19621 gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga cacgacccaa
    19681 tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg tacggcgacg
    19741 ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacgcc gcctaagggt
    19801 aaagcggata aggcggtagc gctgcgtttt gtgtcaaata aaggtctttg gcgctatgac
    19861 atccacgaca tagaaggccg cgttgatccg acactgggaa agcggttaaa agcagatggg
    19921 gatggttgga ttgttcctcc cgcagctgca ccatgggact tcggcttgat tgtgctgcgt
    19981 aatccccctt ctggcattac accgttgccg ttatttgagg gagataaagc cgcgcttact
    20041 gccgcattaa aatcggctgg tcgtaaagtg actcaggcgg gatatcctga agatcatctc
    20101 gatacgttat acagtcatca aaactgtgaa gtgactggct gggcgcaaac gtcggtgatg
    20161 tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt gcataccgat
    20221 gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga tcgctggcgc
    20281 gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca actgtcgcaa
    20341 aaataatgtt caaacgctgc ccgacagtgc gggcagcgtg ttcatcaggc aagtttcacc
    20401 atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt taaccgttga
    20461 ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc ccacaatgca
    20521 taagcgacag acaagtcgat cccttttacg gcctgagaaa gcgcactaaa ggcagccagc
    20581 accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga aaatttcaaa
    20641 aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc gtgaacccat
    20701 tcaaactgcg ccatggttca cctccagttc aggtttacgc gctttacggg tacctgattt
    20761 tatcaacaca atcccggcga ccagggtggt taacccggca attttcatca gcgataaact
    20821 ttcgtcgaat aacataacgc taaacaaggt aataaataaa ataccgatac cttcccacag
    20881 cgcataagct acgcctaagg cgattttttt aacggcgaaa gagagaaata tatacgacag
    20941 agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc tcgcccattt
    21001 catggacagc gtaccggtaa tttctgtagc aatagccaga cctaataaaa tccaataaat
    21061 atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc agcgaaacca
    21121 aagtaaagtg gtcaagcccc gcgcataggg caagcttagg cagaagaaag gactaaagcg
    21181 cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga agatgggtaa
    21241 atgtagaaaa taacgtcctg aacaaattgt ccataatatt acaattatcc gcagtgttgc
    21301 ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat ttgtatcata
    21361 gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga ttttcgttat
    21421 gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg gacaagcatg
    21481 gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg aatgctggtc
    21541 attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat tctcgcatta
    21601 tttattgctg ttatatataa cttatttcgc ataacacatt gatttaatta aataattaac
    21661 accgcaaaaa gtgccaaaaa tcgcaaaatg actacaccag tgactacacc gttcggtgca
    21721 ctgtatgaaa caacgtggaa caaatagaca caagaaatat acaggcggat catctttcca
    21781 gggggaagca caccaattca tgaggggcgt taatgtcgat atggggatcc ccacctaccg
    21841 gaattttttt ccggttaaac gttaatcagg ctggtgggct ttacctgttc ggtaggtgtt
    21901 atgattatcg taataccttt cccccaatgg ggtaaaagca tcaaccgtaa cgaaagtcgt
    21961 tatggttgcc actgataccc caaatttggg tatcacgagt aagccccaaa atctgggtgt
    22021 tactcttact acccaaacta cgggtagttt ccgtagtttc atggtcgagt tgcagatctg
    22081 caactccccc attaaccaca ggcgaatttc cgccttcggt cattccttaa tcatctgcgt
    22141 aatcataatg attattcagc aagcgcaatt ttgcgctttg cttaaagttc agcgagttac
    22201 cgcgccgact tcctcaaatt gaggtttccg aaaatatcag cgggttagcg attaagctga
    22261 ccattaatta aactgcgaaa atgtcgtagt tgtgaggggt gtagccaacc catttttggg
    22321 ccgtcgggaa tatcaaccag ttaccgccgc aaccgctccg gcttcttcca gtggtacgtt
    22381 attttctcct tctcccgata catctccacg cggcgacggt aggccagcaa ctcaagaact
    22441 ctggttcgta tgttgcgcat atccacgcca ttaagttcta taccatcacg gcgcatcacc
    22501 tcagcaacaa cacgcgcata gttttcggct gtcacgctgt ccggctgcgt ggcctgttcg
    22561 tcagcctgct ggctgattcc ggcaacgcgg cggattaatc ccgttatttc ggcttctgtc
    22621 atgctgctga cctcattaca ccccgctaaa ttcttccagt ttctggcggt ggctgtcgct
    22681 tatatcaaaa gcaaaatcct catgctctgc ctggaatgta ccaaacgcca tcagcgccgc
    22741 cacgctcggg tctatcttgt tcggtgattt tttcttgttc ggcttgatat tggcgttcgc
    22801 gtcactctgc atcacgacgt tactcatcga ccaggacaac accggatcgc cacgatgcac
    22861 aatcaccctg cggttaacaa aaacttcgaa cgatttcgcc gccggactga atctgaggta
    22921 ggtttgcggg aacggctcca cctcaaaacc tgccccctgt aattgcgttc tgagatgcgt
    22981 ggcgttccac gtatcaaagc ccaccagcct gatattaaat ttctccgcgt cctgcatgat
    23041 gtcatctctg atccggtcgt aatcaatgca gtcgccaggc gttgtgcgta tccagcctgc
    23101 tttagcccac tgacgataga tggcgcggtt tttattggcg gggttctgta gctgaaattc
    23161 cggcagatag tgacgggaaa ccagcataat ggttttaccg accggaaagg catagcacac
    23221 gctggaaata tcgctggttg atgataagtc cagccccgcg taacactcct ggccgtgtaa
    23281 atcttcctcc gtgaacgttc cggcacactc agcccatgcg ccgttaccca tccacggggt
    23341 agccccctgg caccagatat taaatcgctt tgtcatcatc tccacccact gcgacggaat
    23401 accccgcgct ttctggatgg ttgaggccag tttttcacga tcgacggaaa catcaatatt
    23461 agggttcgcc tttatccaca tcgccggatc atcaacctcg ttttcgtcgt ccagttcgta
    23521 aatcagtaca aaaattgaat cgttaacctc ttcgccgtcc aggatctggc agcaataatc
    23581 atagtgctgc ttacaggctg aaacgacgtt gctgcctgat gtggtaatgg caaataacag
    23641 cccttcggga cgtgcgccca tccccagctc aagcgcggaa taaacgccgt tatcggggtg
    23701 caagtggtat tcgtccacga tggaaaggct ggggtttgtt ccctcgatgg ttgccgcttt
    23761 tgctgccagc ggctttaaca ggctgttgct tttcgggtgt atcaccttat gcgcctgaat
    23821 attcacccgc ctgcgtaacg gtcgggataa aaggcacatc tgacgcgcat catcaaagac
    23881 gatccgcgcc tggtcacggc tcaccgctgc ggtgtagata tcctgctgac cgttctccat
    23941 aatcagaaac cagttagcca gaatcgcggc ggtcgtggat ttcgcatttt tgcgcggcac
    24001 ttcgataaag gcgctcgtgt atttgcgccg tccggtggcc ttaaccttaa agccgaggat
    24061 gcacgcaaag gcgaactgct gccacggctc cagctcaatg ggtctgccac gcatcggtcc
    24121 ttttacgtgc gggcacaccc tggaaaaggc aataaaccgc tccacaacct cacgatcgaa
    24181 cgtgtaaagg gggcttttaa ggtccgaaaa gtaccgttta acggcctgtt tcagccgttt
    24241 acaggccgga attttgcccg tttttacgtc ttctgcgtac ttattccagg cggtcaagct
    24301 cgtcctcttc ttctgtttcc ggtgggtttt tacggcggct tatcgggtca aaaccgagca
    24361 aggaggccat ttttatcatc accctttcag cgtctgattt cgcgcttaat gcggggtttc
    24421 tgctctcgcc gccctgactg ttaacaatgc tgaacccgcg cgccgcaagg tctgcgacgg
    24481 ctttccggta aatggagtag ttgacacaat acagttccag attgctccag tcggcggggg
    24541 tcaggtctcc ccgttccgca agctgccgcg atttttcccg ccactgcttc acggcgatgt
    24601 catccaggta ggcgggggct ttcggtggtc ttgccatgct tatttttcgt cagattattt
    24661 ttcaaaaaat tcccgtgcat aaaaatttga ggaggcgttc ggtgcccggc ggggtcgggt
    24721 ttgtcctgaa aacgcccccc accccgtcat acagcctcat cagcgattgc ggaaacattc
    24781 cataacctcg cggtcacggt cggttaatcg cttcgctgtg atgcgttctg cgcgtcctga
    24841 cgctttatct ttatgccctg tttcctgtgt cttccatgcg tcacgctgcc ttataagtcc
    24901 acggataagg cggttttgtt cccgctcatt catcagcgtc atacatccag ttattgcggt
    24961 tagcggcccg ttcttcctct tcacgaaatc cacctgcggc acgcttgctt tttgttgccg
    25021 gatcaagcca tttcgttttc tggttatgac acgcctggca caatggttga tggttccatt
    25081 cgggccagaa gagaacatca tcaccgccat taatcgggat aatgtgatcc accaccacgg
    25141 cgggcgtata tatccccttc tcaaggcatc gcacgcataa cgggttttta ctcagataca
    25201 tggcgcggta tttgtcccac tgtctggaat atccacgcgc gcggcggtgt cctcgtctgg
    25261 catcctctgc acgccatgcc gcccgcctgt gctcctcaca cttgccggat ttaacgcgcc
    25321 tgttacagcc tggttctgtg catcgtctta atggttgcca cggcatcagt acacccccac
    25381 atcacggtaa gccgtccaga gtgcgcctat cgtcatgggt acgcgtgttt ttgcgttatc
    25441 cgcgacaatc tggcgatttt catacaggtg agcgataaac atcatgcagc caatctttat
    25501 ggctggcgtg aactccagcc cgtcatcaaa gcgcctgcct atgtgtatct ggcacgcctc
    25561 aagcgatgcg gcaatgtatc cgctgattaa ctggtcttca tcgtcgccat cgatgcggca
    25621 atggagtttc acttcttcca gggtgataag ttcttctgtc atttttccgc gccctcacga
    25681 caaagaattt caaggcgtgt cctggcggca tccggcagcg gctgcccgat gatattcagt
    25741 acacgccccg ccagcggccc cgtactgacc tttatccggc tggtggcgtt gatgtccttc
    25801 cggtaccgta tccagatcct tacggtacca atcgccagct ctgcgcccga tgaaatggcc
    25861 tccttgctgc tgaccatgtt cacgcttgcc cagagtgtgt gaccgtcctc ccacgtttcg
    25921 agtatttcgc cggttatatc cctggtctgt ttcagggtct gaatcgttat cctgtcacgc
    25981 agtcgcccta tgttcattcc ggtttttctc cctcgctgat ttttacttcc tgtttccatg
    26041 cctggctgaa ctcatcacca ccatcacgcg gtgacagacc ttcacgttcg cgggcttcgt
    26101 tcggacacat aacgccggat ttgatgccgc gctcataggt ggcaaagcgt tcgccaggtg
    26161 tggcccgtaa caggtccgcg ctgtcaaact ccacctgata ccggatacca ggtacaggcg
    26221 aggccaccag cagggcgttt tttatctgct gctcaaagtt cgccagccac gggcgcatcg
    26281 tcatggtgag aaatgcgcgg cttgcctcgc tgaaattgct gtaggtgctg ttgctgtatt
    26341 cctgcaaaaa aatcggcgag acgttgaaca ttcgggcgat gtcttcaatg gtgaagcgac
    26401 gggaggccag ccattcagca tcctgattac tcatgcccag ctgctgatag ctcatacccc
    26461 cttcaaggat gggcgttttt ccggcgtttc tggccccttt gtagcgttcc agtgcggcta
    26521 atgcctgttt gcccttcacg ccgtccagcc attcgcctga cgtgataacc cctgccgcca
    26581 tcatgccatc acgcatcacg ctcgcgccgt ggcgttgttg ggccagccca agccccagcg
    26641 attcgcggca gatggttaca ggtgagcgcc ccataaagcc gtcatccgtg gagtaacgaa
    26701 ggtgaagtac ttcccacggt aaatagttgc gggtgtttcc ggtgtaggcg tcagtgatgc
    26761 agtagcgcca gttgtgttcg cctgtctgct ccacgttcac cgactgcggc gggtaaggat
    26821 gtaaagccgc cggaaaacca tcacgccccc actgaatcac cgcataagca ttaccgttta
    26881 acaggcagtg gcggatcatc attcgcttaa actggtaggg ggtttgccat gcgttcgggc
    26941 gctcgttgag gatgtgatcg accggatgag aatcaagcca ctcgcgggcc tccttcccct
    27001 tctcattgcg taccaggtac aggtaacacg gcatggtagc caccgcctca gagatgaccg
    27061 tcacggcgtt catgacggcg ggcagtgatt ccgctgtccc cgatgatacg tactcacccg
    27121 cccccgtgtt cgatgtgcca gccagcgcca taaattcatc cagcgtcatg ctgcgctgct
    27181 ctttttttct tctgaaaggc cacatatcac acccccgcta aatccaccca ccagcggcga
    27241 ttatccgcac gcggcatttt ttcggggtgc tgctcataca gggaacggcg ggccagctcc
    27301 acgccggaat cggggtaagc cggtaccgat gtaacggtaa tttcgtacag ttccgccacc
    27361 agcacggtgc gcacgcatgg atctgttgtg gtatcccata catccttacg ggagcgaaag
    27421 ccaaagctca tccccgagat gtccccacgc ttaaccagtt cgataacgtc gcgccctgta
    27481 ctggtatccg gtggggtcag ttcaaagcgt aaccctgttt cgtcctcttc cagtttcagc
    27541 gtgccggaac gggtgcgccc cagtaacatg ctgtggtcat gctcatacag gcctcgaacg
    27601 tcattacccg ccgcaagcca ctcagtaaac gccccccgct ggaatttttc gtaaaactcc
    27661 ccccatagca gttctgaaag cttatcccag cgaacaacgt agcccgtcag cgtaccggct
    27721 ccgctggtag tgatttccga tgaccggatt tccatactct tcataatttt ttcacccata
    27781 aaacactaaa ggggctttta agccccttct gtatgctgtt aatcgtcgtc ctgtggcagt
    27841 tccagaatct tgatcgcgtt cgaatccacc acgccaccgc ctaaatattt ctgcgtgaaa
    27901 attttgatga agcccggctc tgtgaggttg tccggtctgg tgcgaacacc tgtttcgtga
    27961 tcaacaatgt agtaaccgcg tttgaagtca cccagggcaa taacgttatc cggcataaac
    28021 tccagatatt cgaccggaag gcccagcaac gtatcaggat caccagcctg taaacggtcg
    28081 cgccagatgt aatcaccgtt cgcgttcttc accttctgaa gttttgccgc cgtcgtggag
    28141 ttaaccaccc agaccgcgtt tttgcggtat tttttacgta atgcaaattt caggtcgatc
    28201 agcgggtccg cagatgtcca cgcaagagac tcagaaggct taattacctg caacgtacca
    28261 aaatcacgtt ctttgtcgtt cttctctgca cggggtacgg ataaaaagcc ttttgctttt
    28321 ttgtcaccgt cgcccacaac cagatcgctt tcttcggttt ccgtgaaggt gtcgccaatc
    28381 tcacccgtca gccatgaaag gatgtccaca tcggaaaaat ccacgatttc ctgtgtggtg
    28441 cgcgggtacg catagaccgg atacagctta atgctcacct cgttaatctg cggggtgctg
    28501 gtctgttcgc gtgccttacc ctcttcaccg tggttaacgg tcgcaccgcc agcggaaaca
    28561 agctgcttaa actcgttgct gctgattttc ttcacggtac agatgcggcg catggtggat
    28621 tcatccgcca gcattcgcat gatttcggtg ttcagctcgg ggataacggt ataaccacca
    28681 tcagcgggaa cgcctgtact taatgcgcgg gtttcacccg tcagaatgta gtttcgtagt
    28741 tctgcggggt cagtggtctg gctgctttta cctggcttgc tgcgctcttc gtctgcaatg
    28801 gcttcaaggc gggaaatgtc tttatcgagg gattcagctt tagcgcgtaa ttcgtcaaat
    28861 tttgcgccct ctgcatcgtt aaggctgcgg ttttcttttt ccgcgttctc cagcatgtcg
    28921 cgcatctgat ttttaatggc ggttttctgc tggcgtaatt cgattattct cggcataaaa
    28981 aaagtcctgg ggttaagtaa ggaactccag gacgcggcaa aaactcaacc gtttttcata
    29041 aggaaatcag caatcgcacc gatcgttttt ccgcctggta atgaatattg gtgagcacat
    29101 taacaggcgg gaaagtggcc ccagcgtcct ggcaccactg gcgagaataa tcatgattca
    29161 gggcgggtaa aatatgccga tccggtcaat gaacaacgtg gaacgaccac gaacaaataa
    29221 tttacaaaaa atgacaaaaa gccggattgc tccggctgta tgggtgctca gtccctgatc
    29281 cctttcagaa attctatcaa tgcatctatc tgttcaggat ttactgccag catttcaccg
    29341 gatagcgcac atctgacaaa tccatgccga tccttttcga ttagcgcgcc cgtttccagg
    29401 aatgcgcggt aatcattgat gttcatcgtc tccatgttgt cagcatggta tttatcacgc
    29461 tgttttaata tctcatcaaa tttcatcggc attgtttttt cctctgttgt gtctgtttgt
    29521 ttcagatagt aactatgccg gaccgtgacg aaaagccggt aatgcgccat accgtttcaa
    29581 ctggcgcaaa aaaagccgga tttctccggc tgtgtgatta gctgtcctgg taattgcgcc
    29641 gtatttcatc accagcacca tctataccca tttcggcaca agtgcgatta actgcatttc
    29701 tcaggtctcc gaaattatca ggcggctccg gtggcctctg tgccttcctg gaacattcca
    29761 gccgtcgcat cgtgatgtga tgccgttcct tgtctgtctc caccagcatc atgacttcac
    29821 cccatcgcgc cgccgccctc cggtaaaatc ctttcgcttc gagttcttcc gctatgcggt
    29881 catgtaccat cgtcaccccc tcagaacgga atatcatcac cgtaagggtc atcgcctccc
    29941 gctggtggct gattaccctg tgtgcctgtg gttttgcgtc tgctgccgcc tggtcgcacc
    30001 gttttcgcac tgattacgct gtcggcaatg acctgataac cctgacgcgt ctccccgttc
    30061 tgtccggtcc actggcttat ctgcatcgtg ccggatacgc tgattacgtt gcctttctca
    30121 tgtcctgcca gttggtcggc ctgtttgcca aatgccagga ccgacagcca taacgtagcc
    30181 tgcccgtcct gcgcctgact gcatggcagt gataccgcca tacgcgccag cgtcatcggt
    30241 gtgcccttgc tggtctgttt tacctgcggg tcgtccacca gccgcccgta agctgcaatt
    30301 tgtgctgtca tattgccacc tcagtgaagc gatccggcgt tgttcttttt catctcttcc
    30361 atcatttttt cagtgagcat tacgcactga aaacccatgt ttttgatgtc tacgggtttc
    30421 atgcctttgt cagtggatat cacaggcaga atatcctgat acacatcgcg gtcagggtaa
    30481 ctaagcacac aaagcgccca tgatgccacg ggtgaggatc cttcgtctgt ttcaacccac
    30541 cagcctggtg tagctgcgct tatttgcaga atcgtgatcc gtccgttgtg cttgtaaaat
    30601 ttttcaccaa attttggttc gctcatgatt ccacctctcc ggttttaacg ttgatggttg
    30661 ttacctgttc cgcttcggca atctcccgtt ctgtcagcgt ggcaaagttt gcagctgccg
    30721 ttgtcatgaa tgcgcttatt agttcgggat tctccttcgc gtatccttcc cgtgtgtggc
    30781 ggtctataac tttgattgcc acctttaggg aaagctctgt catgtctaac gctttatatt
    30841 tttgctgtgt tctgtctctg cgcattttgg tcatttgtcg cccctgattc atgttttcgg
    30901 tcggcatgtt tgttaagtga ttttgatgta tgcacattta tttttacccc ctcgtttaaa
    30961 aagttttagg ttgtggtgca cctcctctac ctctctacct aatcatcttt caggtcagta
    31021 atggcgcggc tttcagatgg gtagagggct ttcgtggccc tctacccatc atctacccgc
    31081 ccctcacaaa caaccttaat catggtggag ggggtagagg gcttttattt gccttctacc
    31141 agcccctcta cccacttatc atattgaata atgtatattt attttcttta ggtagaagag
    31201 gtagagggct tttgtctaaa attataaaaa cgcgtcgcaa tcctctgttg ttgtcacatt
    31261 ggtttgcgta agtcctttaa ccctgcgcgt tatatattca catccgtaaa cttt
//
LOCUS       DAZGCS010000055        31151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGCS010000055 DAZGCS010000000
VERSION     DAZGCS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.055"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..368)
                     /locus_tag="ACHK01_002637"
     CDS             complement(<1..368)
                     /locus_tag="ACHK01_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFO8097567.1"
                     /translation="MSTLRIGMEIQEFVSHYKNHPVLFIGTGFSLRYLENSFSWDGLL
                     LSVAMELTGNAEFYYDLKAESLEGDDYRYDILATKLEDIFNKKLAEDRNGKFKDINDV
                     FYEHMKKGKKLSRFKIYLTS"
     gene            complement(459..1007)
                     /locus_tag="ACHK01_002638"
     CDS             complement(459..1007)
                     /locus_tag="ACHK01_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO8097568.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(1023..2324)
                     /locus_tag="ACHK01_002639"
     CDS             complement(1023..2324)
                     /locus_tag="ACHK01_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO8097569.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAKNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGKRALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTIVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            complement(2327..3229)
                     /locus_tag="ACHK01_002640"
     CDS             complement(2327..3229)
                     /locus_tag="ACHK01_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097570.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(3226..3375)
                     /locus_tag="ACHK01_002641"
     CDS             complement(3226..3375)
                     /locus_tag="ACHK01_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097571.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(4009..4632)
                     /locus_tag="ACHK01_002642"
     CDS             complement(4009..4632)
                     /locus_tag="ACHK01_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8097572.1"
                     /translation="MSLAARFKARRLELGMTQVEVANSAGVSQQSIESIESGRTRKPR
                     NLLDLAKALKCSPDWLLNGKNIMPLAEISTRRIPILSYVQAGELTEARDITDLTGEFE
                     YILADSDIPETCFALRIDGDSMQPEFKEGDIVIIDPDLCPAPGEFVVAKNDGHEATFK
                     KYRPLGIGIDDFELIPLNPDYPIFRSADMNLQIIGVMIEHRIYRRKR"
     gene            4753..4965
                     /locus_tag="ACHK01_002643"
     CDS             4753..4965
                     /locus_tag="ACHK01_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8097573.1"
                     /translation="MTLATRVKERRKELKMTQVTLAELTGVSQQAINRIESGVIARPR
                     YLLEMSIALDCDPNWLLYGSQNDKKA"
     gene            4969..7161
                     /locus_tag="ACHK01_002644"
     CDS             4969..7161
                     /locus_tag="ACHK01_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO8097574.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTDHLLPVVCNPSATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQRLGELPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSTEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRPDFVDIDIRFDQFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAITWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKMTRIFEAVSQPVPTSK"
     gene            7443..7877
                     /locus_tag="ACHK01_002645"
     CDS             7443..7877
                     /locus_tag="ACHK01_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8097575.1"
                     /translation="MRNIQQVLERWGGWAANSNTTVSWAPIAAGFKGLVVSSSPGRLS
                     CCDDDGLIIDACVCRLQQVRKPEELDVIMLYYVYGLSKREIGRRRRCSEGFIRQQLQV
                     AEGFIEGCLCMLGVSLQMDPEVEIQRDEKSISALRKNCATLV"
     gene            8281..8622
                     /locus_tag="ACHK01_002646"
     CDS             8281..8622
                     /locus_tag="ACHK01_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO8097576.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            8626..9102
                     /locus_tag="ACHK01_002647"
     CDS             8626..9102
                     /locus_tag="ACHK01_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO8097577.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            9086..9610
                     /locus_tag="ACHK01_002648"
     CDS             9086..9610
                     /locus_tag="ACHK01_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097578.1"
                     /translation="MKLTYKIVIAAFFFSVFGALVWSANHYHSKYQAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            9672..10244
                     /locus_tag="ACHK01_002649"
     CDS             9672..10244
                     /locus_tag="ACHK01_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8097579.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            10247..11869
                     /locus_tag="ACHK01_002650"
     CDS             10247..11869
                     /locus_tag="ACHK01_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HFO8097580.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPVLKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWSNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDDLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            11869..13335
                     /locus_tag="ACHK01_002651"
     CDS             11869..13335
                     /locus_tag="ACHK01_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFO8097581.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVMTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRMPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTTAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPESPGGNPISNEPTADSAGGRGYRKWSLRWFK"
     gene            13352..13960
                     /locus_tag="ACHK01_002652"
     CDS             13352..13960
                     /locus_tag="ACHK01_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO8097582.1"
                     /translation="MDALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAE
                     LRNMVDRAPVGQVMKSIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEPFAK
                     EIAASGDVSRSRANLIARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREM
                     EGKFVEWGKPPTLDGMTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            13975..15195
                     /locus_tag="ACHK01_002653"
     CDS             13975..15195
                     /locus_tag="ACHK01_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFO8097583.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVYI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDDGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVNMAFNAVSEIAKGRNTRTTMGDAQRQNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            15200..15703
                     /locus_tag="ACHK01_002654"
     CDS             15200..15703
                     /locus_tag="ACHK01_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097584.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDGSIKVPLGGFMATSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            15715..16656
                     /locus_tag="ACHK01_002655"
     CDS             15715..16656
                     /locus_tag="ACHK01_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFO8097585.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLNGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGDKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETLSYVDGI"
     gene            16697..17065
                     /locus_tag="ACHK01_002656"
     CDS             16697..17065
                     /locus_tag="ACHK01_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097586.1"
                     /translation="MAKEKLVTIHVHTPFTLTLGDQSKQEFGRGRHNVPEEVASHWFT
                     QAHSEFSESVISDTDDLQPIIDGLQAQIADKDKLIADLREALLKLQEQNDSLQAQIAA
                     AQTGGNGAKDAKESKPASGK"
     gene            17031..17438
                     /locus_tag="ACHK01_002657"
     CDS             17031..17438
                     /locus_tag="ACHK01_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HFO8097587.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADVLLSE
                     NVTGKELFPYFAELFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            17435..17989
                     /locus_tag="ACHK01_002658"
     CDS             17435..17989
                     /locus_tag="ACHK01_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097588.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPSEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLTPGVKSVEGQTVPQLKAAAQAALDGNASGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGAKAELARRAAGESPGTDLV
                     KPLIDTGQYRRAITHVVRDKNADS"
     gene            17976..18365
                     /locus_tag="ACHK01_002659"
     CDS             17976..18365
                     /locus_tag="ACHK01_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HFO8097589.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGSGFVQAHCELLPFDGGIPVEQ"
     gene            18340..18903
                     /locus_tag="ACHK01_002660"
     CDS             18340..18903
                     /locus_tag="ACHK01_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097590.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            18907..20052
                     /locus_tag="ACHK01_002661"
     CDS             18907..20052
                     /locus_tag="ACHK01_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HFO8097591.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            20063..20503
                     /locus_tag="ACHK01_002662"
     CDS             20063..20503
                     /locus_tag="ACHK01_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HFO8097592.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGSDGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            20507..20959
                     /locus_tag="ACHK01_002663"
     CDS             20507..20959
                     /locus_tag="ACHK01_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8097593.1"
                     /translation="MEFEIKGVKYRAAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIETVKFEQLKPVFETMLPRIAEELSSLTEDDTEAIIHPCLAVVSRRHMDGWV
                     PVFTKGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            20971..21147
                     /locus_tag="ACHK01_002664"
     CDS             20971..21147
                     /locus_tag="ACHK01_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP pyrophosphohydrolase"
                     /protein_id="HFO8097594.1"
                     /translation="MPDGLSYLLNPVDAGLIPYTALKDGSVDLYDIALLNDHLAVKAD
                     NQRRIEKWREDNER"
     gene            21137..23125
                     /locus_tag="ACHK01_002665"
     CDS             21137..23125
                     /locus_tag="ACHK01_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFO8097595.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GTALSVVAFTAKIASGLDNLYWASQRTGATVHGIQSIGYAVSQVGGSADAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMASIFTGVGQKLSGMPYYRANQYAQMLG
                     IDENTLMAMRRGVGGFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSS
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKGSVNELVKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQYAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            23125..23712
                     /locus_tag="ACHK01_002666"
     CDS             23125..23712
                     /locus_tag="ACHK01_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO8097596.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVLISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            23712..24014
                     /locus_tag="ACHK01_002667"
     CDS             23712..24014
                     /locus_tag="ACHK01_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097597.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLVVVCDDNTQDYPTKTDLGGLSHLLVSTE"
     gene            24017..25084
                     /locus_tag="ACHK01_002668"
     CDS             24017..25084
                     /locus_tag="ACHK01_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097598.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSASDVGTVREVDAADVGQRDGR
                     NYGLIFSGEIRYSVTGKDSPVDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVV
                     NNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTISQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLRSASSLQREVG"
     gene            25084..25437
                     /locus_tag="ACHK01_002669"
     CDS             25084..25437
                     /locus_tag="ACHK01_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097599.1"
                     /translation="MKKWIVIGLCFLPGFAFAANPGGVTLQCGGYKLELIPDSLFRIN
                     GETVTSQKIKTLGNGNGMKADMGLMPAKDGNNYGFEFIRRPGTETRFLNVQLLQNSMD
                     APRIIGSFPCKKVDD"
     gene            26212..26964
                     /locus_tag="ACHK01_002670"
     CDS             26212..26964
                     /locus_tag="ACHK01_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HFO8097600.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIIQSFDPDAVT
                     AVVQPAIRSVEKDNDGNRITQNYPLLVDVPVIFPRGGGCTLTFPVEAGDECLVIFADR
                     CIDFWWQNGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTGKIKIVAPGGLDVVTPLADFSEKVTIHGLLTWLGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            26964..27317
                     /locus_tag="ACHK01_002671"
     CDS             26964..27317
                     /locus_tag="ACHK01_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097601.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            27317..28516
                     /locus_tag="ACHK01_002672"
     CDS             27317..28516
                     /locus_tag="ACHK01_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8097602.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNTAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMVTATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGTPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDNGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLMIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            28513..29193
                     /locus_tag="ACHK01_002673"
     CDS             28513..29193
                     /locus_tag="ACHK01_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFO8097603.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            29193..30038
                     /locus_tag="ACHK01_002674"
     CDS             29193..30038
                     /locus_tag="ACHK01_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8097604.1"
                     /translation="MAKNDFKAFATDRNANVISQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPT
                     NKLPDLRGEFIRGWDDGRGIDTGRALLNWQPHTILDHAHYMELWTGDGLAAGSAREGV
                     NPGILATYGDGGIVKTDEPGLKVPSSLRALSSRSVKRYGEISENVGTETRPRNIAFNY
                     IVRAA"
     gene            30038..30640
                     /locus_tag="ACHK01_002675"
     CDS             30038..30640
                     /locus_tag="ACHK01_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8097605.1"
                     /translation="MNKAVLNSELIAIKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSADLNSWEYVPDHRGEVVYSTETGDTKEITVPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAA
                     RKLTQTETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(30612..31031)
                     /locus_tag="ACHK01_002676"
     CDS             complement(30612..31031)
                     /locus_tag="ACHK01_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8097606.1"
                     /translation="MDRYFYSQKENGFFTDLNKAPSDAVEITTDEWLLLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALADLKKRKLINEANEHMNSRQWPGKAAIGRLKSE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
BASE COUNT         7695 a         7301 c         8465 g         7690 t
ORIGIN      
        1 attgaagtta aataaatttt gaatcgactt agtttttttc cttttttcat gtgctcatag
       61 aaaacatcat tgatgtcttt gaacttaccg ttacggtctt ctgccagctt tttattaaaa
      121 atatcttcaa gttttgtagc taagatatca tatcgatagt cgtcaccttc taggctttca
      181 gctttcaaat cgtagtaaaa ctcagcatta ccggttagtt ccattgcgac actcaaaaga
      241 agaccatccc agctaaatga gttttctaga taccgtaggc taaatcctgt tccaatgaat
      301 agaacagggt ggtttttata gtgacttaca aactcttgga tttccatacc gattctcaaa
      361 gttgacacgt aatacctcta taaaaacaaa tagccgtaaa aattccaata gctttttgga
      421 tctcgcccgg cgtgtaaccg ggcgtctatg aagaagggtt aaatcagtgc ttcagcatca
      481 gcaccttcgc tgatatcgtc gaaatcgtca gcgcttgcca ctccgccgcc agcgaatgca
      541 tcgccgtctc gcaggaactg gactccgccg agtgaggcat taatgcgttt accgaaatta
      601 ttgtcctgtg cccagatatc gataacggcg tttacatagc accctgcata gggacgtcca
      661 tcagcctgaa ttagtggcga acgatcgcga tcaagaacag ctgggcgcgc tttgttagca
      721 gcgttcagga agaaattgcc gggaaagcct tcatactctg ctttttcatc accatcatgc
      781 aggcacagat tgagtttttt ctccagctgg ttataaatgg actcccactt ctccccccat
      841 ttttccttcg ctacctgctt catagcttta cggatttctt ccagttgtgg gtgtttggga
      901 gacattaaaa atactgcgga gaaacgcgga tcgccttcgc cgtttacagt tttagcttca
      961 aacagagacg ggaaggccag acgaacattg ttcagcttca gtttcatggg gatttcctta
     1021 aatcagatga ggtctgcggt tagcgtatcg tcggatacgt cgtcgaaatc atttacaggg
     1081 ttgatattga gtgcggggcg tgggtctgac tcgggaacga tggtgggttt accatcagct
     1141 cgtgttatca gtgcctcgac ttttgaccaa cggcgcggac tggccttttt gataagtttt
     1201 tcggcttttg tgggactaat aagtttaaag tcgaatactt cttcagtttt gtacctgaac
     1261 tggtccttca gaagtgcgcg agctgcctct tcgtcactcc aggcccggtt accttgtttt
     1321 cctgttacca gtttaaaccc cggtaccgga tgtccggcat tgagttcatt gtgaacccgg
     1381 tcccgtactg cctttagcca ggattcaata aagtcggcct ggctatagat ctctgcaagc
     1441 tgctcaatgg ttaacagagg tacacgtgcg ctggcattgg tgattatttc gctgacaggc
     1501 tttgtcagat cttcaaaatc gctggccgct gtttgtaaat gctgcatttt ctgggcagtg
     1561 caaatagctt ttgctttaca gaagcggcac tgtttttctc caggtatgaa gttttccagt
     1621 ggtagtgtct caatgccttc gcattcagca atattgagaa caaggatcgc actggttgcg
     1681 gcctccagcg cccgtttacc gaaagactga agttcctgta cggttaacga ccattctgaa
     1741 acgtggttga gccttggttg gtgaataaat aatcttacag tttcaaagtc atacagcatg
     1801 ctgaattgtt caagcgcacc cagagcatac agttgtagtt gctcattttg ttctgcatca
     1861 atgcggacgc ctttgccata tttcaggtcg tggatttgta attcgttacc agcgatgatt
     1921 atgccgtcgg cagttccgaa agattcttcc acacccgtta tatgtgagaa atcaacacgt
     1981 tgttcaacca atagttcatt attctttgca agagtccaga ccgtatcaac ataccggccg
     2041 acggcttcga ccatttcatc atccacctgt gggccagatg tatcatcagg attttcgcga
     2101 agggggtatg agccgagaaa catagaaaca ttgcatccgg cgtagtgttc cgggtggctt
     2161 tgcctgtttc gtagaacttt ttcagcaagc gcgtgcgctg cagtgccctc gattgcaaaa
     2221 gttgtttctt tatccggttg tgtggcctcc agcgccagac ttcctgggca gcgcatccat
     2281 cgatgcgctg atgatggaga aagttgtgca tgaacgtctg gcatgattaa ccctccagtg
     2341 ctttttcagc cagggtgatt acttcagcga gattttcatc cgttacttca ccaagtttcc
     2401 tggctccctg tttttccaga attgcaatag cttctgcccg gtaaccccct tttgctaact
     2461 ggaggatcaa cccttcagct tgtttgcgta gtgccgcgaa atcaattgta tggtcatctt
     2521 tggcgtcatt attctggctg gaatttgctg cgtctctgcg tgcaaattct tcctgcagct
     2581 gaaggtactc aacacggttg atctcgatat ggcctttttt aagcatctcg ttcaacttgc
     2641 gtaaggtgtg gagttcactg gctgctgtgc cggatacatt tttgacgtaa aacggccccg
     2701 tgcgttctcc atctttgtta ctggcctttt tcggcttaac ttcatcacgc ccatctgcag
     2761 gtgcatcaag tagctgctcg gcaaaagcac gtcgctcgcc gatggttggc aggtcgtccc
     2821 agaacttaag aatgttacgg gacaggtcca ggagagcagg tttaagcagc gccctggctc
     2881 gtttgacgcc ctgtaatgcg ctgtcgagag catcaatctg aactactcgt ttatcgcctt
     2941 cagcatcacg gtaggcaaca gcacgttgca gcatgtcttc tgtgataggg gtggctaccg
     3001 ggtagaaacc agccagtgcg ataacgtcgc tgaactccag atcatccagt gtcattgccg
     3061 ctgacatgtt ttcagcttca gttgctgtat cccgacattc ctgcactcgt gaaatcgtgt
     3121 caggatgcat aacaatgcct gatgccattg tgcggataag acgttcaagc agcgcattat
     3181 gttgtgccag aagttgatta ttaagttcta gactggtttc taaactcata ctgtggtcct
     3241 cgctacaagg agaatgaaag tgatgatcag accgagcgca gtggcaacgg ccagaccggt
     3301 catcaaatcg aagtttttac ggcgataacg gagaacatcg cgccccgtca gtcgatggat
     3361 gtgttcaggt ttcatcggtt gtattccttt tttcatatcg gggagcacgc tgttgcgagt
     3421 gcgctttcag acataaaaaa gcccgtcacg tgaggcgggc aaagactaca cacagcaatt
     3481 acatggatga ttcagttggt attgtgcgta cgtggtacca caaggttgaa gcggacacgc
     3541 cagtgcgttg caatggtatg caggaaagca ccacatccag ggcagtgctc ataacattcg
     3601 ttgatttggt atcgttttgg ctttggtgtc agaagctgtg tttggcaagc agggcattca
     3661 cctttgagcg gtttatacgt tttcacgccg tttcctcatt cagttcactt tggtggttcg
     3721 gcggcttcga cttgtatcag tagacagtgc ttcgccgcac cccaaaggga acttactgac
     3781 ccgtattgcc gacatcctgt cccgccacgg tcccgacgca tggtttagag tcgcgccgtt
     3841 cgacttgtgg ctaaagatac aatttgagtt gtattgtgta aaccacaaat gtggtatttt
     3901 gtgaggtgtg aatactactt tattgatatt taagtgaatt taatttgtaa gactttacaa
     3961 atggggtaag ggaggctgtt acgcctcccg atatttacgg tgggaatact aacgtttacg
     4021 gcggtaaata cggtgttcga tcattacacc tatgatctgt aagttcatat ctgcactacg
     4081 aaaaataggg taatcaggat ttagggggat taattcaaag tcgtcgatgc cgattcctaa
     4141 tggacggtat tttttaaatg tagcttcgtg accgtcgttt ttggcgacaa caaactcccc
     4201 tggtgcagga catagatcag gatcgatgat aacaatgtct ccttctttaa actctggttg
     4261 catgctgtcg ccatcgatac gtaatgcgaa gcatgtttct ggaatgtcag agtctgccaa
     4321 aatatattca aattcccctg tcagatctgt tatgtctctt gcttctgtaa gctctcctgc
     4381 ctgaacgtaa cttaatatag gtatgcgtct ggtacttatc tccgcgagag gcataatatt
     4441 tttaccgttc agtagccagt ctgggctaca tttcaaggcc ttagccagat ccagaagatt
     4501 gcgtggcttt ctggttcgtc cgctttcgat ggattctatg gattgttggc taacccctgc
     4561 agaattcgct acttccactt gagtcattcc taattccaaa cgacgtgctt taaaacgggc
     4621 tgcgagagac atgggattag tccttctggg ttgtattaat gtgtatcccc acattaaaaa
     4681 caatttttgt tgtatttgac aaacctcatt tgttgttgct aaataccact aaaattgtat
     4741 gaggtgaaag caatgacctt agcgacccga gtaaaagaac gacgtaaaga actcaaaatg
     4801 acgcaagtta cgttggctga gctcacagga gtgagccagc aggcaataaa cagaattgaa
     4861 agtggtgtta ttgctcggcc acgttatctt cttgaaatgt ctatagcatt ggattgcgac
     4921 cctaattggc tgttgtatgg ctcacaaaac gataaaaagg cttaacccat gtccgatagc
     4981 aaaccatggg gagctacgcc tgatgagtgg tttcatttcg acctggtatt ggggagaact
     5041 gatcatcttc tcccagttgt atgtaacccc agtgcgacca tatcccctga tagtaaactg
     5101 aaagcgttgg gtaagacgcc gagtcgctat aaccgggacc gccaggtcac cggtattgct
     5161 caatggaccg ggcatgttgt tactgagcat gattttgccc gctggtcgaa tgaaccggat
     5221 tatggcatct gcgtgcgtac aggccatggc tggctggcgc tggactgtga tagcgaagat
     5281 gaagacattc aggcagatat tcgcaaaacg cttgtgcaac gtctgggtga gttgccgccg
     5341 cgacgctggc gagcaaacag taataagtgt ctgtatctgc tggccgttga tggtgatttc
     5401 cgtaagcgta tccatcgcct ggcgggggat atgggcatta tcgagttgct ggcgaacggg
     5461 cagcagttcg ttgcctgcgg tacgcacagc agcggcgcgc gtattgaatg ggacggcggt
     5521 ttgccggatg aacctccggc tattactggt gagcagcttg aaacgctgtg gcagcgcctg
     5581 gctgaacaac tccctgtgtc ggtaaccacc gaagcgggca acacgaagat gcgcgaccga
     5641 tcagcattca cgcccggcgc gacggatgat acagctgaat atcttgatgc caatggctgg
     5701 acgctgctgg atggcgcaaa tggtgaacga tatatccgct gtccgtttga agacggccac
     5761 agtagcgggg gcgatccaac aagcacagtt tattttcctg cgggaaccgc gggctttgag
     5821 caggggcatt ttaaatgcct gcatgccagt tgtgcgcatc gtgatgacgg agatttcctt
     5881 aatgccatcg ggatccgcaa cgacgatttc gaagatctga ccagcaccga agtggcggaa
     5941 cctttaccgc tgcctgcttt cgagcgtgat aaatgggggc gtatcgaggc aacaatcagc
     6001 aacgcagcca aagcagtagt acgccctgat tttgtggaca tcgatattcg ctttgaccag
     6061 ttccgcgacg aaatcatgtt tgcccctgca ggatccggac aatggcgggc attcactgat
     6121 gcggattatg cgcgcctacg catcacgatg gaaaagcggg gatttaaacc tgttggtcgt
     6181 gaacttattc gcgatgtggt gttacttgca gccgatgaac aaccattcga ttcagcgatc
     6241 acctggctga acggactgga gtgggatggc gtgccgcgca tcgaatgttt ctaccatacg
     6301 cacttcggta ccgccgacac gccttatacc cgtgcggtgt ctatgtacat gtggaccgcg
     6361 ttggcggggc gagtactgga gccaggcatc aaagcggata tggtgccgat cctcgttggt
     6421 ccgcagggct gcggtaagtc ttccggagtg gaggcactga gccctgatcc tgcgtttttt
     6481 actgaaatct cttttgccga aaaagacgat gatctcgctc gaaaaatgcg tggtcggctg
     6541 gtggcagaga ttggtgaact gcgcggactt aataccaaag agctggagtc aatcaaagcg
     6601 tttgtgacgc gtactcacga aaactggatc ccgaaatacc gggagttcgc cacccagttt
     6661 cctcgtcgcc tggtgttcgt tggtaccact aatgaggacg aattccttgc ggacaagact
     6721 ggtaaccgtc gctggctccc cgtggaagtg tcgaaagtcg acgtgaaagc gataaaaaga
     6781 gatctccttt tactttgggc tgaggctcgt gaggtgtttc agcgtctggg gggtatccag
     6841 ttccgtgagg ctgaacaact ggcagcgagt gtccatgaac agtacaccat caaggatgct
     6901 tggcttgaaa cggtagagaa atggctcgac acgccagacc tgatgactaa tgaacttccg
     6961 cgaaattgcg aatttttacg cgcaagtgat gttttgcgtg atgcgattgg gctaaatcct
     7021 gaccgcatcg gaaaacgcga agaaatgcga attagtaatg ttttgcaaaa ttgcgggtat
     7081 aagcgtgccc aaaggcgaat tggggggaaa atgactcgaa tttttgaggc ggtgtcccaa
     7141 cctgtaccaa cctcaaaata gaggttggta cattttaact aattgaattt aaaggccagt
     7201 accaactgta ccatctgtac caacctaatt actaagaacc ccatatatat atataagtcg
     7261 cttggggaaa aggtttgaaa agagctggta caggtgggta caggttggta caggccgaac
     7321 aaggaatttt ttgcacataa cagcgtgtaa taagcgaatc ggaactgcgt tatccacacc
     7381 cacggataaa caaacgtggt tctcagaaaa ttttttcgta gcaaaacgta gaggtcagag
     7441 ctatgcgtaa tattcaacag gttttagagc gctggggtgg ctgggctgcc aatagcaaca
     7501 ccacagtgag ctgggctcca atagcggcgg gatttaaagg gctggtagtc agcagctcgc
     7561 ctggcaggct gagttgctgt gacgacgatg gcctgattat cgatgcttgc gtctgtcgac
     7621 tgcagcaggt ccgtaagcct gaggaactgg acgtcattat gctgtactac gtctacgggt
     7681 taagcaaacg tgagataggg cggcggcgtc gctgttctga aggatttatc cgccagcagt
     7741 tgcaggtagc tgagggattc attgaggggt gtctctgcat gctgggtgtg agccttcaga
     7801 tggatcctga ggtcgaaatt caaagggatg aaaaaagtat tagtgcgcta cgcaaaaact
     7861 gcgctacgct ggtatgagtt gaatttctga cctcaacgaa gagctccaaa tcattggggc
     7921 ttttttaatg ctttattctc agtaaaagtt aataaaacag ggttttcgtc gcgaaaaaac
     7981 gctatgcagt ttttgccctt ttttatgcac cttttattca ctcgaatttc gccattctgg
     8041 accacttaag ttgattaaat aggcctttca tcgcaaatct attgcgagcg gtgatcgtgt
     8101 ggttcctata acgtacatta tgttaaataa cttccttttt taacaaattt aacaaggttc
     8161 gctatggcga actttttttg tattcagggc ccaccgaagg acggctcata acccaatcct
     8221 acgggcgtat acgcagggcc cgcctttcaa caacaccccg taatggcgga ggtgggaagt
     8281 atgaaaatgc acaatgctcc tcattcctgg cctgacttac tggaactctt acaaagttgg
     8341 tggcgtggag atacgccgtt gggcgcagtg gttatgtcaa ttgttatggc tggcttgcgc
     8401 attgcctatt ttggcggtgg cggcggctgg aaacgaaaaa cgcttgagat tttgctctgt
     8461 ggtgctctga cgctgacttt tgcatccgct cttgagtatg tcggatggcc taaatcgctt
     8521 tctgttgcca ttggtggtgg tgttgggctg atcggtgtcg atgctattcg tggggctgca
     8581 atgcgagtaa tcggtaacaa gtttggtggc tctaaggagt aattcatgca gacactaaat
     8641 tcccaacgta aagctttcct ggatatggtg gcatggtcag aaggaacgga taacgggcga
     8701 caaccgacac gtaatcacgg ttatgacgtt atcgtcggag gtgagttgtt cactgattac
     8761 tccgatcacc ctcgcaaact tgtcacgcta aacccgaagc ttaaatcaac agccgcaggc
     8821 cggtatcagc ttctttcacg ctggtgggat gcctaccgca agcagcttgg cctgaaagat
     8881 ttttcgccag aaagtcagga cgctgtggcg ctgcagcaga ttaaagagcg tggcgcttta
     8941 ccgatgattg accgtggcga tattcgtcag gcaatcgacc gttgcagcaa tatctgggcg
     9001 tcgttacctg gtgcaggtta cggtcagtat gaacataaaa tcggtgacct gatttccagg
     9061 tttaaagatg ctggtggggt ggtaaatgaa gctgacttat aagattgtca tcgcggcatt
     9121 tttcttctct gtctttgggg cgctcgtctg gtctgcaaac cattaccaca gcaagtatca
     9181 ggcagaaaag ttgcgggctg ataaagcgga aggtgaagct gaatatcaag ggaaagtgat
     9241 agctaatcag gcattaaact tcaatcgttt taaccagata gcagaaaacg caagccgatt
     9301 aaattctctg gtcgacatcg gtcacgagaa gacagtcatc aaataccgtg aggttctgct
     9361 ccgtgaaaag aactgtgatt tccctgttcc tgttgatatt gctgtcgggt tgctcaacta
     9421 cgcgaaccgt ttacgcgcca gcgcattgca cgccgattcc ggggacattg actcagccgg
     9481 tgatcgtgcc actaccacca gaacgttgac atattgccag gctgttctgt ggattaaccc
     9541 actgttggca gccatcgaga aggcgaataa ccagttggct ggtgtccgac aaatagaaca
     9601 gtcccggtaa tagcattaca gaagctcttc caggaggggc ttcgataatg acctgataac
     9661 tggaaaataa aatgactaag aagctgaaag caaaacacga ggtgttttgt cgcgagtttc
     9721 ttgtcgatct gaatgctaca caagcagcta ttcgcgcagg ctacgtctcc aggcgagcac
     9781 atgttacggg ggctgaacta tacggtaaac ctgagatacg cgcccgtatt aacgagctaa
     9841 agcaggagcg tattgatcaa ctgggcattg atgcgaatta tgtgctgatg cgactggttg
     9901 agatcgacag gctcgatgtg gctgacatcc tggaggacga tttaagtatt aagcctctgt
     9961 ctgcgtggcc ggaatcgtgg cgtcggtacc tgagtggatt taacctcgct gaaatgtttg
    10021 aggggcgagg agatgacaga gaaatggtcg ggatccttaa aaagattaag tggcctgata
    10081 aggttaaaaa ccttgagttg cttgggcgtc atgtttctgt tcaggcgttt aaagacaacg
    10141 tcaaaaatga agtgactggc gctgatggag gaccagtcag aacagaaatt accaacttaa
    10201 cgccggagca ggctgcagag gcgtatagaa aaatgatggg ctaagtatgc cgttaccatt
    10261 ccccttcgat tttaaacatc ctgattacca gatggttttt gaatggcgga tggaacgcct
    10321 acagcgcatt cgccagaatc ctgaaatatt gcccgtgttg aagcagtttt accgaaccaa
    10381 tccggctcag ttcatcatcg actggggcat gacaacggac ccgcgtaata ttgattatgg
    10441 cctgccggtg accattccgt ttttactctt ccctaagcag gaggagtgga tccactggat
    10501 tatggaacgc tggagcaatc gggagaatgg tattaccgaa aaatcccgtg aaatggggct
    10561 cagttggacc gcgatcggac tggcctgctc gctttgtctc ttcaacaaag aaatggttat
    10621 cggtttcggc tcccgtaaag aggaatacgt cgacagcacc ggtgacccga aagcattgtt
    10681 ctggaaggcg cgcaagttcg tggaaacact acctgtagag tttcgcggtt cgtggagcga
    10741 gaagaagcac gcgccatata tgcgtgttga gtttcctgaa actggtgccg ttatcaaagg
    10801 cgaggctggc gataatattg gtcgtggtga ccgtaccacg ctttatctgg ttgatgaggc
    10861 tgcattcctt cagcgtcctc tgctgattga tgcggcgttg tcacaaacga cgcgttgccg
    10921 tattgacctg agttcagtta acggcatggc gaacccgttc gctcagaagc gtcatggcgg
    10981 gaagataccg gtattcacat tccactggcg ggatgatcct cgcaaggatg aagagtggta
    11041 tcgcagggaa tgcgagaaaa tcgataatcc ggtggtggtg gcacaggaac ttgatctgaa
    11101 ctacagcgca tcagcggaag gcgtcctgat tccatccgaa tgggtacagg ctgccgttga
    11161 tgcgcatatc aaactgggta tccagccaac aggcaaacga cttggcgcga tggatgtcgc
    11221 cgacgaaggc agggacaaaa atgccttttc cacccgtcat ggcttcctcc tggaaaatgt
    11281 gcgggaatgg tccggtgtgg gcagcgacat ttatcagtcc gtcgagaagg tcttcggctt
    11341 ttgcgaacag gacgatcttg aagagtttcg ctttgacgag gacgggctgg gcgctggcgt
    11401 tcgcggcgat gcacgcgcta tcaacgaact gcgtaacgct gcgcgtcgac cgtcaatact
    11461 tgccacaccg tttcgaggta gtggcgcggt atttgatccg gatgatgaag ctgttcgcgg
    11521 ggacaacggg caagcagcac gtctgaacaa ggacttcttc gctaacgcca aagcccagag
    11581 ctggtggcgg ttacgtaaac tttttcagaa tacctggcgc gccgtggttg aaggtatggc
    11641 ttacaacccg gacgaaatca tctcaatcag cagtagcatg gcactcaaag ataaactcat
    11701 catcgagctt tcgcagccga cctattccat taatggtgtg ggaaaaatcg ttattgataa
    11761 acagcctgat ggaacccgat cgccaaacct tgccgactcg gtgatgatca actatgcccc
    11821 aatgaattca gccctgaaca tctgggagct gctagggaga caggcctgat ggcacgaaac
    11881 aaacaagccc tgcggcgaac tgcgcaggcc acagctgatg gttatgagaa ttttattgcc
    11941 cgcgtaggga tgcagacacc taaccagcac tcagcatcca cctaccgggc taatttcacc
    12001 agtcgtaacc gcatgctggt ggaatggtcc tatcgttcgt cctggatcat cggcgaagcg
    12061 gtcgatgcta tcccagatga tatgacccgc aaaggcattc gcatcacttc ggaaattgat
    12121 gcaaaagatc gcggcattct cgaatcacaa ctggatgagt tgcaaatctg ggatgcgctg
    12181 aatgacgtgc tgaaatggtc gcgcctctat ggcggtgcgg ttggcttcat catgattgag
    12241 gggcaggcac caatgacccc gctgcggctc gaaaccattg gagaaggcaa gtttaagggc
    12301 attctcccgc tcgaccgctg gatgattaac ccggtgatga cccgccgcat taaagagatg
    12361 gggccggatc tcggcaaacc tgagttttac gatgtggtga ccactgccac gggcattcca
    12421 gcctggcgca tccatcacag ccgcctgatt cgcttcgacg gggtgacgct gccattccag
    12481 cagaagatga ccgaaaacga atggggaatg tcggttgtag agcgtatctg ggatcggctt
    12541 actgcgttcg acagcgccac tgtcggcgcg gcgcagctgg tctacaaagc gcatttgcgt
    12601 acctacagcg tggagaagct acgcgagctt atcgcacttg gtggtcctgc gtatgaagcg
    12661 ctgctgaaga acatcgacct gatccgccag ttccagagca atgaaggcat gacgctcatg
    12721 gactcgcggg ataagttcga aacccaccag tacagcttca gcggtctgga tgacattctt
    12781 tcgcagttcg ctgagcagat cagcggtgcc gttggtatcc cgctggtgcg actgttcggt
    12841 caatccccga aaggcttctc tactggtgac gcagacctcg ccaactatta cgaccgggtg
    12901 agctcattgc aggaacgccg cttacggatg ccgatgcgtc ggatactgga catcatgcat
    12961 cgttcggagc ttggcaagcc gctgccggac gatttcacgt ttgagtttaa cccgctatgg
    13021 caaatgtctg acgttgaccg ctcaacggtg gccgtaaaca ccaccaccgc gatcagtacg
    13081 gcgctgggtg atggtctgat gacactgaaa gccgctatga ctgatttgcg cgaaaattct
    13141 gacgtaaccg gcatcggggc gtccattacc gacgaggaca tagagaatgc cgaagacgaa
    13201 gcgccgccag gcatcggcga acttggcgac aaacctccag agtcgccagg cggaaatccg
    13261 atatcgaacg agcctacggc agatagcgcg ggcggtcggg gatatcgtaa atggtcgcta
    13321 cgatggttca aatgacagtg tcaccgaaat aatggatgcg ctggagcgct acagcgaaat
    13381 catcaccccc tgggcgacga aggttgctga gaactttacc gcagacatag cgcgccagaa
    13441 tgaaaagcag tggcgtcagc acagccggaa catcagtgca gagctgcgca atatggttga
    13501 ccgcgccccg gtaggccagg tgatgaaatc catcgttgcc gagcagatta agtacatcaa
    13561 atcgctccct cttgaggccg ccgatcgggt gtatgacatt cagaacaaag ccatcgaggc
    13621 cgttgtgact ggtggccgcg ctgagccatt cgcgaaagag atagctgcgt ccggtgacgt
    13681 gtcacgctca cgagcgaacc ttatcgcccg tactgaactt ggacgtgcaa ccggcgcgct
    13741 cgatcaggcg cgtgcgctgt caatcggctc gaatggttat atctggcgta cagccgaaga
    13801 tggcgacgtc cggcattctc atcgggagat ggaaggtaag tttgtcgaat ggggaaaacc
    13861 tccaacgctt gatggcatga ccggtcacgc tggcgagctc ccgaattgtc gctgttataa
    13921 agaaatcgtt tttcccaacc ctcattctta tctcgcctga atcgcaggta aaccatgaaa
    13981 tattttttca atacccggct gggggaaacc cgctatcagc tggctgacgg ctcgctgctg
    14041 tgcaaagacg tgccgatagg tcgaacgggt aagcagctct acggcgctgc cgatctgcca
    14101 aacctcaaac ccgacaagct cggtgagata gtcgtaacgc gttctcctga gcaggtattc
    14161 catccggcca cgctcgcctc attcgaaggg atgagcatca cgatcctgca tcctgaagat
    14221 gaaaacggga atgtgcggct ggtaaatccc gagaactgga aagagcttgc ggtcgggcac
    14281 ctccagaatg tccggcgcgg gacgggtgag cagtctgatt tgatgctggc tgaccttatc
    14341 gtcaaagacg aaaacgccat tcagcttatc gaagatggcc tgcgtgaagt atcgtgcggc
    14401 tatgacgcgg agtacgagca gaccgagcca ggtaaagccg agcaggtcta tattaccgga
    14461 aaccatgtgg ctcttgtccc caaaggcaga gccggaaatc gttgtgcaat tggagacaga
    14521 gacacaatgg caaatcaaaa gaaaaactgg tggaaccgca tgcgtgcagc catcaagaca
    14581 ggagatgccg acaccatgaa cgaactggtg gagtcggctc ccgcatcggt tacaggagat
    14641 gatggggatt tgccgcaggg cgttaatctc aacatcaacc tgtccccgca gcaaccacta
    14701 ccggacaaag caccagagat gggtggagat ccaaccggcg acagtgatga tgacctcaaa
    14761 acattactga aagccctgct ggctaagctg gaaggaaatg cgacgggcga taacgacaat
    14821 aagcctgacg ataatccgac cggtgacggc gaggacgatg aagaggaaac cacgattact
    14881 ggtgactcag cctggcgtgc cgaagttatc gttccgggta tcgatctgag ccgtaagatg
    14941 aaaccgaccg cgttcaaacg cgaggttctg gcttctgctg acaaaacgct ggttcgccag
    15001 atagtcggtg atgcggatat ccgcaaatta ccgaaacaat cggtcaacat ggcgtttaat
    15061 gccgtgtcag agattgccaa agggcgaaac acccgtacca ccatgggcga tgcacaacgc
    15121 caaaatatgg gcatgaccag catcgcttcc ctgaacaaac aaaacgccga attctggtct
    15181 aaccgcaaag gataatccaa tgactgcata tctgtaccgg atgcctgttg gcattgccgg
    15241 ggctatctct cgcccgcagg acttaaccgt cgaaccggtg gtccttaaat ccgataacgc
    15301 ctttgctgcc tatgggctgg ctggtaaata cgatgctgac ggttttttcg taccgctggc
    15361 agatggtgat accgcagaca aggtgaaggg gatctatgtg cgcccttatc cgaccacgtc
    15421 gcagccggac atggttcgcc aggtggggag tggcaagaac ttcccgggcg acgccatgaa
    15481 gcgtggctac gtgaccgtta atctcggttc tgattttgat gccagcacca tcaaaaaagg
    15541 cgacccggta tacgttgtcg tctccactga tggatccatc aaagtgccgc tgggtggatt
    15601 catggccacg tcagtcagtg gcaaaaacgt ggtgctgacc aacgctgaat tcacaggtgc
    15661 cggtgatgct aacggcaatg cagaaatttc ctggaagatt taaggaacag acgaatgatt
    15721 acttttgatc aggcaaccgt tgacagctct ggtgcctttc tcatcgggga gctggagcga
    15781 ctcgaccaga cgctgaacct gccactggtg gggtacacct ggacccgcga tattcagttg
    15841 cgtgaagatg tctccatcgc agatgacatt tccagctgga cgaataccag cttcgccgct
    15901 gcgggtactg gcgcaaatcc gaatggcaaa aactgggtag gcaaagactc aaccgctatt
    15961 gctggcgtga acgtggatat cggcaaatcc ggtaacccgc tgaacctgtg ggggatggag
    16021 cttggctgga cggtcataga attgcaggct gctcagcagg tcggacgccc gattgatacg
    16081 cagaagtatg acgggatgca actgaaatgg cagatggata acgatgaaca ggtgtatgtt
    16141 ggcgattccg cattaaacct gaaaggcctt gttaccctga acggtgttcc tgtcaacaac
    16201 gctgccaaaa cgtgggcaac ctcaacaccg gacgaaatcc gcgcaagcat taaccaggtg
    16261 ctgtctgatg cgtgggccgc ttccggttac tctgtggttc cgcgtgattt gctgatcccg
    16321 cctgagcagt ttgctctgtt gtccagcatc atcgtttcat ctgctggtaa ccagtccctg
    16381 ttgacgtacc ttcagaccaa caccatcagc tatcaccaga acggtgttcc gctgaatatc
    16441 cgcgcggtta aatggctgaa aggccgtggt gtgggggata aggatcgcat ggttgcgtac
    16501 accaacgata aaaaatacgt ccgctacccg ctggttccgc ttcagagcgt gccggtgcag
    16561 tatcgcggtc tgtatcagat cgtcacttac tacggcaagc tgggtgcggt tgagccagtg
    16621 tataaagaaa ctctgtccta tgtggacggt atctgataac cagaatggcc ccgaaagggg
    16681 cctgaaggaa actgaaatgg cgaaagaaaa gctggttacc atccatgttc acaccccgtt
    16741 tacgctgacg ctcggcgatc agtcaaaaca ggagtttggc cggggacgac ataacgtacc
    16801 ggaagaagtc gcgtcgcact ggttcaccca ggcgcactct gagttttccg aaagcgtgat
    16861 tagcgacacc gatgatctgc aacccattat cgacggcctg caagcgcaga ttgccgacaa
    16921 agataagctg attgccgatc tgcgagaagc gctgctcaag ctgcaagagc agaacgacag
    16981 tctgcaagcg cagattgctg ccgcccagac tggcggtaat ggggcgaaag atgccaaaga
    17041 atcaaagcct gccagcggta agtgattttc ggcgcgactt tccacagttt gctgaccctg
    17101 ccaaatatcc cgaagcacaa atccagtttc gtctgaatct ggctgatgtg ctgctgagcg
    17161 aaaacgtcac cggcaaagag ttgtttccgt actttgccga gttgttcgtg gctcactaca
    17221 tgacgctatg ggcggcagac agccgggcaa tgctggttgg cggcccgggc ggttcaacca
    17281 atggtgttca atcctctaag tctgttgaca aggtaagcgt cagctatgac accagcgcga
    17341 cgctgaatcc tgatgcaggt ttctggaata acacccgata tggcgctgaa ttttatcagt
    17401 tgatcacgat gtttggtgca ggcggtcgcc agctatgagc ttcaaaagcg gtgtaacaac
    17461 gagggtggat aacgctcagg ccattctgga tgcgctcagg tcgctaacca aaaaggatgt
    17521 gctggtcggc atcccttcgg aagacagcga gcgtgaagat gttccgtttg gtaatgccgg
    17581 gatcggctat gtcaacgaat acggctcacc agcgcaaaac atccccccac gcccgcacct
    17641 gacccccggc gttaaatcgg tagagggaca gacagtgccg cagctcaaag cagcggcgca
    17701 ggctgcgctt gatggtaatg cgtcgggtgc ggaaagagcg ctcaaccgtg ccggaacgct
    17761 ggccgctaat ggcgtcaggc gttacatgac tattaccggc tttacgccgc ttgctgacag
    17821 cactgttgaa gcccgcgcac gtcgaggtcg caaaggggca aaagcggaac ttgcgcggcg
    17881 cgctgctggt gagtctcctg gaaccgatct ggtgaaaccg ctaatcgaca ccgggcaata
    17941 tcgcagagcg attacccatg ttgtgaggga taaaaatgcc gactcttgat gtaacagatg
    18001 tgctttttga ccccgatttt tgcgacttca atttgtgggt aacacgccga gtgcaaacgg
    18061 tggatgagga cgggatcggc agcgacagcg aagttaaaaa gcagtttgcc ggagtcgtaa
    18121 ctgttgatcg ctctctggaa aaccgccgta tgcaggcagg gcaggtgatc agcggtgcaa
    18181 ttctgattgt gacgactgag agactgacgc agggacagac tggccgtgat gccgatatcg
    18241 tgacgtatca gggccgtgat tatcgtgtga ctttcgtcga cccgtataca gcttatgggt
    18301 ccggattcgt tcaggcgcat tgtgagttgc tgccgtttga tgggggaatt ccggttgagc
    18361 aataacacca gcacagagcg cggatggctg ataccaacca gtggcgatcc ggattatgac
    18421 gaagcgctcg acaggctgtt aagccagtgg atgcgtaacg tttccggtct gtctgccggg
    18481 atggttcgcc cgcgctggca gaaagagcag ccgccactgc taccggttga aacgaactgg
    18541 tgtgcgtttg gggttatcgg atggtcaggt gatgacagtc cggcattcac cagacagacc
    18601 gatgatggct ctcagctctg gcggcatgaa acgattgagt gtatggcttc gttttatggt
    18661 ccggcgggga tggtgtatgc gtcccggttt cgtgacggta tatctgtacc gcagaacaac
    18721 gcagcactga atgcgctggg gctgtctctt ggcgattaca caggtctgac tcccttccct
    18781 gaacttatta atcagcaatg ggtccgtcgc tacgatatga cggtgcgtct gcgccggaag
    18841 gttgtgcgcg agtacggtat taaatcgctg gtggaagcac cagtcatctt tttcggagat
    18901 taagctatgg cacagggctt gcctgtatca aacgttgtta atgttgatgt gatcatgtcg
    18961 ccgcgtgcag catcagggcg aaattttggt gcattactca ttctcggccc gtccacaatc
    19021 attccggtaa gtgagcgcat tcgtcgttat tctgccgcgg aagatattgg aaaagatttt
    19081 ggcgtggaat caccagaata taaagctgcg caggtgtttt tctcacaatc accgaaacct
    19141 caggaggttt ttgttggtcg ttgggtgaaa acgaagggag acagcgaaca ggccacgcct
    19201 gagacgctgg agcaggctgt gaatgccatg cttgattata cttcatggta tgggctgggg
    19261 attgcagacg atgcagatat tccggatgca gactggctga aagtggctgc ggcgatcgaa
    19321 tcctcttctg taagccgtat tctggcgatt acgacaagcg atgagaaatg cctgcagact
    19381 gcatccagcg atgatttggc atcaaaactg aaaaccgccg gatattcacg cagttttatt
    19441 cagtattcat cgggtaataa atacgctgcg ttatctgcat ttggccgggc attcacggtt
    19501 aatttcaatg gcagtaatac cgcgattacg ctcaagttta agcaggagcc gggtgtcggg
    19561 tatgaaacac tgacagtcag ccaggcatcg gcacttgatg caaaaaactg caatgtgttc
    19621 gtgtactacc agaatgatac agctatcctc cagcagggag tgatggctaa cggcgatttc
    19681 tttgatgaac gccacggcct ggactggtta cagaattatg tgcagaccaa cctctataac
    19741 ctgctttata ccagcaccac gaaggttccc cagactgaag ccggtattac ccgactgtta
    19801 tcaaatgttg aaaaatcact ggatcaggcc gttcagaatg gactgattgc tccgggcgta
    19861 tggaacgggg gcgaccttgg tcagttgtca tcaggtgaca cactgcccaa aggttattac
    19921 gtatacgccc agccgctgga tgaacaggca caatcagaac gtgaagcccg taaggctccg
    19981 gtgattcagg ctgcaataaa acttgcaggc gcggttcatt acgctgacgt acagattaac
    20041 gttgttcgct aaggggaagt gaatgtctac ctattctttt atggatgtca ctgcgacgct
    20101 gaccgggccg accggttcga ttgacctcgg gtacggttct gcaagttctg aagaggggat
    20161 tgtggttgcg atgggcggtc ctaaaaacac catgaccatc ggttctgatg gcgaagtgat
    20221 gcacagtctc catgcagata aaagcgggac gattaccgtt aaccttctga agacatcacc
    20281 gacaaataaa aaattgtcgc tggcgtataa cgcacagagc cagtcttctg ccacatgggg
    20341 gaataacgtt attgtgatcc gaaacaaggt cagcggcgac atcatcacgg cacgtagtgt
    20401 tgcgttccag aaacaaccgg ataatgccaa cgctaaaacc ggtaatacga tgccgtgggt
    20461 gtttgactgc ggcaagattg accaggttct cggggagttt taatacatgg aattcgaaat
    20521 caaaggcgtg aaatatcgcg cggcaaaact cagcgttttt gaccagctga aagtgacccg
    20581 caaacttctg ccggtactgg cgggaatgat gtcagatttc gggagcattc gctcccgttt
    20641 gcctgctgac ggcaaaatcg aaaccgtgaa attcgagcag ttaaaaccgg tgtttgaaac
    20701 catgctcccg cgtatcgctg aggagctgtc ttccctgacc gaagatgaca ccgaagcgat
    20761 tattcatccc tgtcttgcgg tggtgtcgcg gcgtcatatg gacggatggg tgccggtatt
    20821 taccaagggc gaactgatgt ttgatgatat tgacctgctg gtcatgctgc agctggtggc
    20881 gcgggtggtc gccgattcgc tgggaaattt tttgcctaca ccccttacca gcacgacgca
    20941 gagcctgcaa cagggctgac gtttaacagc ctgccggacg ggctgtccta ccttctcaat
    21001 ccggttgacg ccgggttaat tccttataca gcacttaaag atggctctgt cgatttgtac
    21061 gacattgctc tcttgaatga ccatctggcg gtaaaagcgg ataaccagcg gcgcattgag
    21121 aaatggagag aggataatga acgctgaaac tattaaagat ttcctcgtct cgcttggctt
    21181 cagtgtggat gatgcaggag cgaaaaagtt cggttctgtc ctcgccggta caactgcaaa
    21241 tgtcatcaaa atggggctgg ccgtcgaagg aactgcactg tccgtggtgg ccttcacggc
    21301 taagatcgcc tccggtctgg ataatcttta ctgggcgtca cagcgcaccg gcgcgacggt
    21361 ccatggaatt cagtctattg gctatgcggt ttcgcaggtt ggcggcagtg cagacgctgc
    21421 gcgctcttct ctggaaagcc tctcccggtt tattcgtaac aatcccggtg cagagggatt
    21481 tctgaatcgc ctgggggtac agacacggga tgccagcggt aacatgcgtg acatggcctc
    21541 tatttttaca ggtgtaggcc agaagctcag cggcatgccg tattaccggg ctaaccagta
    21601 tgcgcagatg ctgggcattg acgaaaatac ccttatggcg atgcgccggg gtgtgggtgg
    21661 cttctccggg cagtacagcg caatggcgaa agctatcggc ttcaatgctg acgaggcggc
    21721 cagaagctcc aacaaattta tgacctccct gcgcgagttc ggcgcgatgg caggcatggc
    21781 ccgtgacaaa atcggctcta atcttgctgg tggtctggcg ggttcgctgg acacactgcg
    21841 ccgccacatc ctcgataact tcccgcgcat cgagcagacc ctgacgaaag ccataaaagg
    21901 cattctggcg ctcggagaca tcatcgggcg gctgttcttc aggctaattg agggaacatc
    21961 cagccttatc acctggtggc aatcgctgga taagcaaacg cgggagctca tctcgctgtt
    22021 tggcgcgctg acgattgcgc tgcgcattct gaacagtacg ttctggatgt cgccgattgg
    22081 cctcattacc gcgctggcgg cggggattgc ccttctgtgg gaggactatc agacctggaa
    22141 ggaaggcggc gacagcctga ttgactgggg caagtggaag ccggaggtcg atgccgcgct
    22201 gaagatggtt cgtgacctga aagggtctgt taatgaactg gtgaaagcgc tggcgaaact
    22261 gctcaatatt gaccccaaat catggtccct gaagtgggat ttcagcaact tcatcgacca
    22321 gatgggcgaa ttcagcaaaa tgctgaacat gatcgccgac ctgctcaacg ctatcaaaga
    22381 tggccgctgg gctgatgccg tcagcatcgg caaacagata cttaatcagg gcagcgaaaa
    22441 tccgtcagcg atgccgatgg ttacagacag cgctaacagt actgccgact ggattaaaga
    22501 gcactgggga ttcgatcccc gcagtgtggg ccggacggta cgcggctggt ttggtgatga
    22561 tgagccggaa caatatgcac aggctacgaa acgaggagaa cggaataaca atccgggaaa
    22621 ccttaatttt gctggtcagg caggggcttc tcttgaacgc ccgggcgggc gatttgccag
    22681 atttgaaact gcttttgatg gattacgggc tcttgctcgt cagttaatgc tgtacgccgg
    22741 acggggaata aacagtgtgg agaaaattat ctctacctgg gcacctgcgt ctgataataa
    22801 caacacaact gcgtatatca gggctgtatc gcaacgactg ggagtggatc cccgggctgc
    22861 cctgaatatg agcgatccgc aaaccatgtc agcattgatg agcagcatta tccagcatga
    22921 gaatggaaga aatatctatt ctcgagagct gattaataag gctgccgtgg cgggaattag
    22981 tggcaaaatg acagaggtta accagcaaaa tacctaccac atttacggtg gtggagatcc
    23041 gcacgctgtc ggtaatgagg ttgcacgtcg gcaacagtct gcaaatgctc aggtcatgcg
    23101 aagtaatcag gtgagggtgg gttagtggat attctctcta cactttttca tcagcagagc
    23161 agaaaaatag gaatgattgt tcccagtgtt gttatttcag agaagcatac agatatgctt
    23221 gaaataacag agcatccggt agaggttggg gccgctgtcg ctgatcatgc ctataaaaaa
    23281 ccgtcagaag tggtgatgga ggttggtttc gccggtggcg gcgcattgct ggattttgcc
    23341 agtaatctga cggctaccag cctgctcggc ctgagtcctc agcagacgta tcaggagcta
    23401 ctggatctgc aggaaagccg tatccccttc gatgtggtaa ccggtaaacg actgtacagc
    23461 aacatgttga tccgggcgct ggaagtgacg acggacaaga caaccgaaaa cgtcctgtcc
    23521 gccgtcctca ccctgaggga ggtccttatc tcccggacgc agcagattac cgtcgcggat
    23581 aaaaccaaca tgaaggaagg ggccagcacg tcggcggtac agaacagcgg caacaaaacc
    23641 accaagcctc cagatacttc actgctgaaa agcatcacgg gtaacgtggc gtcattactg
    23701 gggggcggct aatgacaatt caggaaattc cgctgacagc ggacaaccag cagttcagca
    23761 tcgtcctggg tggtgtcacc tggcggatta gcatcatatg gcgcgatctg tactggatta
    23821 tggacctgca gaacgacaga ggggagccgg taatctccgg tattcctctc gtcactggtg
    23881 ctgacctgct ggcgcagtac gcctgtatgg gacttggttt taagctggtg gtggtctgcg
    23941 atgacaacac acaggattat cccacgaaaa ctgatctggg cggcctcagt catttactgg
    24001 tatcaacgga gtaagcatgt cacagaactg gatgagacat ttcgagctgc agcttgtgga
    24061 cgggaacggt cagggaattg agctaagtga tttcaaagtc acctttacga tcgactggtt
    24121 caacatcagc agcgcgtccc gggtagggac tatcaaaatt tataacctct cggcagatac
    24181 tgtgaaccga atcaccgggc aggaattttc gaaagtgcgt ctgattgccg gttacgacgg
    24241 tatcgcgccg gaggtgtcag caagcgacgt agggaccgtg cgggaagttg acgcggcgga
    24301 cgtgggccag agagatggcc gcaactacgg actgattttc agcggtgaaa ttcgctactc
    24361 ggtcacagga aaagacagtc cggttgattc ctacgtcctg attcaggcag cagatactga
    24421 tctggctttt gccaccagta taacctcaca gacgctggct gccggttaca cggtcgctga
    24481 tgtgaaccgt gcgctgatga aagacttcga agccaaaggt gcgaccgaag gcctgacgcc
    24541 tgaaatgcct gctactgtat tcccccgggg gcgggtgctc tttggcatga cgcggcatct
    24601 aatggataac gtagccgggc aatgtggcgc aacatggcaa ttcgtggatg gtcagcgcca
    24661 gatggtggtg aataatgaat atgttcacga agcgattgtg ctcaacagcg ctaccgggct
    24721 tattggcatg ccgcagcaga ccatcggcaa cggcgtaaac gtccgcgcgc ttattaatcc
    24781 gaacatccgg gttaatgggc tcattcaact ggatcaggct tccgtatatc gcaccgcgtt
    24841 gtcgaacaac gatatcgcta tggctggtgg gcagatcacc gaccagaaca cggacggaaa
    24901 tattacgcta agcggcacca tatcgcagcc tgccagcatc gcaacggatg gcgtttatat
    24961 tgtgcgcggg attatgtaca ctggcgacac aaggggccag gcgtggtaca tggatatgat
    25021 gtgcgaagcg cgcggtgcgg cggatcttcg ttcggcgtcg tctttacagc gtgaggtagg
    25081 atagtgaaaa agtggattgt tattggactc tgtttccttc cggggttcgc atttgctgcg
    25141 aatcctggcg gtgtcacgct tcagtgtggt ggctacaaat tagagttgat tcctgattca
    25201 ttgttcagga ttaatggtga gacagttacc tcccagaaaa tcaaaacgct cggcaacggt
    25261 aatggaatga aggcggatat ggggcttatg ccagccaaag acggtaacaa ttacggtttc
    25321 gagttcattc gccgtccggg taccgaaacg cgtttcttga atgtccaact actgcagaac
    25381 agcatggatg cgccgagaat cataggttcg tttccttgta agaaggttga cgattaatac
    25441 aatatgtgca atcaaaggaa tgaggctgtt ggccttgaag tagatacgat tttgtcgtac
    25501 tcgagagaca ggtatgaaga agttaagaca acatacaggc ggttagagga caaagctaat
    25561 tttcttcttg ctgtgcttgg tgttgagatt tctgcattat tagccgtttt tgggtccttt
    25621 gagctgggac aacagttaaa gactagtcta tccgcaagat tgtctctatt gtgtctatgt
    25681 tcatgttttg tatgtttggt tttttgtttt tattatttat ggaaatgttg ggaacttaga
    25741 aatattccca aaatgcctgt gcatcgaaca cctgaacaac atgaatttct tctgaggggt
    25801 agtagagatg acacagtcag atttcatttg ataatgtatg gtagggctat tgatagaatc
    25861 gagaaaatcc ataaagaaaa atcagcgttt gtaaatgagt tgtttcgatg gatggttatg
    25921 tcatttgtat tttttattat ttctgtaacg tgcatgttaa taagtaaggt ttgaatatgt
    25981 cctgtccaga agaccaaaat gaaccacaac ctaaaccaag tgagcctgag gtctctttcc
    26041 tagataacta tgagcaagag attatggcta gagtaccttt cggggatatc gaaattatga
    26101 cggaaagcta tcacgacaag ccttcaactt ttcccaagaa agatagttag tgaagttctt
    26161 ataaatcaaa cccgccacca ggcgggtttt ttgctttctg gagcctacta aatggccgta
    26221 tctgaccaga cccgcagcgg cgaccttgcc gaaacattca aatctgaacg ggaaacaaca
    26281 aagaaccaga tccgtgtcgc cttgcctggc attattcagt cattcgatcc tgatgcggtg
    26341 acggcagttg tgcagcctgc tatccgttcg gttgaaaagg ataatgacgg caaccgcatt
    26401 acccaaaatt acccgctgtt ggtggatgtg ccggtgatat ttccgcgcgg cggcggctgc
    26461 acgttaactt tcccggtgga agccggcgat gaatgcctgg tgatttttgc cgatcgctgc
    26521 atcgatttct ggtggcagaa cggcgggata caggagccgg ttgatgacag aatgcatgat
    26581 ttatcggatg cgttttgtat tgtcggtccc cagtcgcagg caaggaagat tagcggtatt
    26641 aataccagtg ccacacagtt gcgtagtgac gatggcagca cctattttga gcttaatcct
    26701 gataccggga aaattaaaat tgtcgctccg ggtggtcttg atgtggttac ccctctggct
    26761 gatttttctg agaaagtaac cattcatggc ctgttaacct ggttgggggg catggtgggg
    26821 tctgtggttt ctggtgtggc ttcaaaaatc actggtgctg ttgagttttt ggggagcgtg
    26881 aaggctaacg gcaagccaat cgatgatacg cacactcatg gtggtgttca gcgcggcgga
    26941 agcaatactg atggggtaaa ctgatgcgat acagacgtga agacgccgat ggcgattaca
    27001 cctttggcag cggtgatgac acctggctga ttaactcgcc tgaggccgta gcacaggctg
    27061 tgaaaacgcg attcgaattg tggtatgggc aatggtttct cgacaccacc gaagggactc
    27121 cgtggatcca gtctgtgctc ggtaagcaga agccggaaac ctacaacctg gcgatccgta
    27181 agcgcatcct cgaaacgcgg ggcgttaaat caatcctctc tttcaatacg acagtggata
    27241 ccacgacccg acgtgtcatg ttttccgctg aaatcgacac tctttatgga ataacgactg
    27301 ttacatcgga ggcgtaatgg ctctgaacct tgattctctc ggtttatctg caaaggtaac
    27361 cgcggagggg atcagtgcgc ctgattatca gacgatactc agcaccctga ttagctattt
    27421 tcagcagatt tatggtagtg atgcctacct cgaaccggat agtaaagacg gccagatggt
    27481 ggctctgatg gcgctggcga ttcatgatgc caataatact gcgataactg tctacaactg
    27541 tttttcaccg gcaaccggct atggggccgc actgaccagt aacgtgaaaa taaatggtat
    27601 ttcacgtaaa ggcgcgacga attctacggt tgatttgctt cttacaggaa ctgccggaac
    27661 aaccatcatt aatggcagcg tgaaagacag taataatgtg atatggcgtt tgcctgcttc
    27721 agtggtggtc ggcgtggatg gtacagtgat ggtgaccgca acatgttccg tcagtggtgc
    27781 agtggcggcg ctggctggaa ctatcactga aattaatacg ccaacccgtg gctgggtttc
    27841 ggtaactaat cctgctgcgg ctactgtagg cactccggca gaaactgacg ctgagttacg
    27901 tatccgccag tcgcaaagtg ttgcgttgcc atcaataacc ccatttgaag cactggatgg
    27961 tgctgtttct aatgttaccg gtgtaacccg ccacaaactc tatgaaaacg ataatggttc
    28021 ggaggacggt aacgggttac cgccacactc tgttgctgta attgtggatg gcggtgatgt
    28081 gacggatatt gctcaggcta tcagagggaa taaaggccag ggaacagcca ctcacggtac
    28141 aacatccgtt acggttccgg ataaatacgg caatccccat gtaatcaaat tttcgcgttc
    28201 cagtgatgtg cctgtttatg cccggattaa attaaaagtt tttacgggtt atacctcaca
    28261 gatagggcag cagatccagc aggctatttc cgactatatc aatagtctga tgattggtga
    28321 ttcggtcctt ttaagtcgca tttactcacc ggcgaatctt ggcgtggtga gtggcgggaa
    28381 tgcacgctat tacgatattc aggaactgac gattgggaaa tccccggggg ctttgtcgtc
    28441 atcaaacatt gatatcagat acaacgaatc tgcgtcctgt accccggaaa atatcgttat
    28501 aacggtggag tcatgagcaa atacaccgaa ctaatcacga actaccacgc caccaaacct
    28561 aaatttcttg cacatgttga tctgatgacc cggccgctta ttgatgttgc ggctgccacc
    28621 agagggctga ttactgcatt tgatattgac tctgcggttg gtgtgcaact tgacattctg
    28681 ggattgtgga tcggacgtag ccgtgttgtc agccagccta tttcaggtgt ctatttcagc
    28741 tgggataccg acgggcttgg atatgatcag ggtgtatggc aggggccata cgatcctgat
    28801 tccggataca tgtacctcag cgatgaaact tatcgtgtca tccttaaagc gaagattgcg
    28861 attaataact gggacggacg gaatgattcg cttccggcaa ttcttgacgc ggcaacagca
    28921 ggatccggac tgcgaatgca gatagtcgat aaccaggaca tgacgatatc ggtctgggtc
    28981 tttcctgata ctgatatttc agatgtatcg cgtgagttaa ttgcggcaat taaacagggg
    29041 tatctcacag taaaagccgc cggggtatgg gcgggtggca ttgaaacacc ttcggtggaa
    29101 accccatcgg aaggctcaaa attttttggt tttgatatgg ataacgaatt catcagtggt
    29161 tttgatgtag gagcatgggg agtattactc tgatggcgaa aaatgacttt aaagcgtttg
    29221 caacggatcg aaatgccaat gttatatcgc aggaggaatg ggaagcgttg cccgcgcttt
    29281 tatctggatt tacagcaggg aaagcctcca gtgcgcaagt caataaggtt attcggcagg
    29341 ccagctttat tgctgcagct ctggcccagt ttgtaagtga taaaacgcaa cgggatgtgc
    29401 ttgataatgg tgatctgccc ggttttgttg aattgctggg atcggggttt gctgttgaat
    29461 acctgagccg caagaatccg tttggcgata tcaaatcgga tggcacggtg aaaacggctc
    29521 tcgaaaacct tggtttggga gaaggctctg cattaccggt tggggtgcct gttccgtggc
    29581 cttcagccac tccgccgaca ggctggctga aatgcaatgg tgcggctttt tctgctgaag
    29641 aatacccgga actggcaaag gcttacccga ccaataaatt gcctgattta cgcggtgaat
    29701 ttattcgtgg ctgggatgac ggtcgcggga ttgatactgg tcgcgctttg cttaattggc
    29761 agccacacac aattttggac catgcacact atatggaatt atggacaggg gacggactcg
    29821 ccgcaggaag tgcacgggaa ggcgtaaacc caggaatact ggctacatac ggtgacgggg
    29881 gaatagttaa aacggacgaa cccggtctta aggtgccttc ctcactacga gctcttagct
    29941 ctcgtagtgt taaacgttat ggtgaaatta gtgaaaatgt aggtacagaa actcgtcctc
    30001 gtaacatcgc ttttaattat attgtgaggg ccgcatgatg aataaagctg tattaaatag
    30061 cgaactcatt gccataaaag cgggagatat taccgtttat aattatgatg gtgaaacgcg
    30121 ggaatatatt tctacatcaa ctgaatatct cgctgtcggc gtcggtatcc cggcatgttc
    30181 ctgtctggat gcaccaggta cacataaagc tggttatgca atctgtcgtt cggcagattt
    30241 aaactcatgg gaatatgtgc cagaccatcg cggtgaagtt gtctatagta ccgaaacggg
    30301 agataccaaa gaaatcacag ttccgggtga ttaccctgaa aatacaacca ctatcgcccc
    30361 attaacgcca tacgataaat gggatggtga gaaatgggtg accgatactg aggcacagca
    30421 tagcgccgca gtagacgcgg cagaagcaca gcgtcagtca ctgattgatg ctgcaatggc
    30481 ttccatcagt ctgattcagc tgaaattgca ggccgcacgt aaactgacgc agacagaaac
    30541 aacccgactt aacgccgtgc tggattacat tgacgcggtg actgcaacag ataccagcac
    30601 cgcgccggat gtcatctggc ctgaactgcc ggaggcgtag gccattcaat atctggcgca
    30661 ctggaggtat caaccagttc cagtgcgtcc agataatcca gccataaatt atattgcgcc
    30721 agttcctcac ttttcagacg accaatagcc gctttacctg gccattgtct gctgttcatg
    30781 tgctcgttgg cttcattaat aagttttctt tttttcaaat ccgccaatgc aataaggttt
    30841 tcttttgata aaggtggttg ctctgtcaaa accggatatc cctcctgatt gctgactatt
    30901 ttcatgccat tatcctgacc atccagtagt aacagccatt catccgtggt tatctcaaca
    30961 gcatctgaag gtgctttatt taaatcggta aaaaaaccat tttctttttg tgaatagaag
    31021 tatctatcca tttattaatc tccaaaagca atccagtaag caaaaggatt tattccttgc
    31081 tcagtcacag atgacatcag ggaaaattgc gatggtgaaa caggtaacgc tgcaaaacta
    31141 accattgttg a
//
LOCUS       DAZGCS010000056        30379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGCS010000056 DAZGCS010000000
VERSION     DAZGCS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.056"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..1703
                     /gene="yegH"
                     /locus_tag="ACHK01_002677"
     CDS             120..1703
                     /gene="yegH"
                     /locus_tag="ACHK01_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8097607.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2154..4007)
                     /gene="asmA"
                     /locus_tag="ACHK01_002678"
     CDS             complement(2154..4007)
                     /gene="asmA"
                     /locus_tag="ACHK01_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO8097608.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTYVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4029..4610)
                     /gene="dcd"
                     /locus_tag="ACHK01_002679"
     CDS             complement(4029..4610)
                     /gene="dcd"
                     /locus_tag="ACHK01_002679"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO8097609.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4702..5343)
                     /gene="udk"
                     /locus_tag="ACHK01_002680"
     CDS             complement(4702..5343)
                     /gene="udk"
                     /locus_tag="ACHK01_002680"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO8097610.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5661..8978
                     /gene="dgcE"
                     /locus_tag="ACHK01_002681"
     CDS             5661..8978
                     /gene="dgcE"
                     /locus_tag="ACHK01_002681"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8097611.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9017..9874)
                     /gene="alkA"
                     /locus_tag="ACHK01_002682"
     CDS             complement(9017..9874)
                     /gene="alkA"
                     /locus_tag="ACHK01_002682"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO8097612.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10008..11360
                     /gene="yegD"
                     /locus_tag="ACHK01_002683"
     CDS             10008..11360
                     /gene="yegD"
                     /locus_tag="ACHK01_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8097613.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11372..13312)
                     /gene="yegI"
                     /locus_tag="ACHK01_002684"
     CDS             complement(11372..13312)
                     /gene="yegI"
                     /locus_tag="ACHK01_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO8097614.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13309..14070)
                     /gene="pphC"
                     /locus_tag="ACHK01_002685"
     CDS             complement(13309..14070)
                     /gene="pphC"
                     /locus_tag="ACHK01_002685"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO8097615.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14067..14726)
                     /gene="yegL"
                     /locus_tag="ACHK01_002686"
     CDS             complement(14067..14726)
                     /gene="yegL"
                     /locus_tag="ACHK01_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8097616.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14947..15006)
                     /gene="ibsA"
                     /locus_tag="ACHK01_002687"
     CDS             complement(14947..15006)
                     /gene="ibsA"
                     /locus_tag="ACHK01_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO8097617.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15279..15335)
                     /locus_tag="ACHK01_002688"
     CDS             complement(15279..15335)
                     /locus_tag="ACHK01_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8097618.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15607..15663)
                     /locus_tag="ACHK01_002689"
     CDS             complement(15607..15663)
                     /locus_tag="ACHK01_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8097619.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15942..17189
                     /gene="mdtA"
                     /locus_tag="ACHK01_002690"
     CDS             15942..17189
                     /gene="mdtA"
                     /locus_tag="ACHK01_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO8097620.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17189..20311
                     /gene="mdtB"
                     /locus_tag="ACHK01_002691"
     CDS             17189..20311
                     /gene="mdtB"
                     /locus_tag="ACHK01_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO8097621.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SCFARHEEEA"
     gene            20312..23389
                     /gene="mdtC"
                     /locus_tag="ACHK01_002692"
     CDS             20312..23389
                     /gene="mdtC"
                     /locus_tag="ACHK01_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO8097622.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTADEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRSSLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRMRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23390..24805
                     /gene="mdtD"
                     /locus_tag="ACHK01_002693"
     CDS             23390..24805
                     /gene="mdtD"
                     /locus_tag="ACHK01_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097623.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24802..26205
                     /gene="baeS"
                     /locus_tag="ACHK01_002694"
     CDS             24802..26205
                     /gene="baeS"
                     /locus_tag="ACHK01_002694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO8097624.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26202..26924
                     /gene="baeR"
                     /locus_tag="ACHK01_002695"
     CDS             26202..26924
                     /gene="baeR"
                     /locus_tag="ACHK01_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO8097625.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27115..27447
                     /gene="yegP"
                     /locus_tag="ACHK01_002696"
     CDS             27115..27447
                     /gene="yegP"
                     /locus_tag="ACHK01_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO8097626.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27656..27952
                     /locus_tag="ACHK01_002697"
     CDS             27656..27952
                     /locus_tag="ACHK01_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8097627.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27954..28250
                     /locus_tag="ACHK01_002698"
     CDS             27954..28250
                     /locus_tag="ACHK01_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8097628.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28353..29714
                     /gene="yegQ"
                     /locus_tag="ACHK01_002699"
     CDS             28353..29714
                     /gene="yegQ"
                     /locus_tag="ACHK01_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO8097629.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30044..30361)
                     /gene="yegR"
                     /locus_tag="ACHK01_002700"
     CDS             complement(30044..30361)
                     /gene="yegR"
                     /locus_tag="ACHK01_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO8097630.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7019 a         8101 c         8024 g         7235 t
ORIGIN      
        1 gatgttataa tcctcaaata atgtgagata aatagtgccg caacattgca tttttgccct
       61 gatttatcca tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta
      121 tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac
      181 tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga
      241 agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac
      301 tgctggcgtc aatctcctgg ctggtcaccc tgacgcaacc gctgttcagc ttccgctcgt
      361 ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag
      421 ccacgatgga gctgaacgaa cggctggaag gaaaagacag caataacccc acacaacgca
      481 aaggtgcgaa gttctggggg gtggtgacac aaattgtggt actggacgcc atcttctcac
      541 tcgactcggt gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg
      601 ttgttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca
      661 gtcacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg
      721 tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgccgcc attggcttct
      781 cggtgatgat cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg
      841 ctaaccagac gctacgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa
      901 aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc
      961 agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta
     1021 ctgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg
     1081 aagaagaaat ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttactgatg
     1141 gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtctc
     1201 tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccactggtg ttcccggaaa
     1261 ccttgccgtt gttacctgcc ctcgagcagt tccgtaatgc ccgcacacac tttgcttttg
     1321 tggtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca
     1381 ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga
     1441 atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg
     1501 tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat
     1561 atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa
     1621 cgttgcaggt ggaaagccat cgcgtgcaga aggtgcaaat tataccgcta cgtaaggatg
     1681 gcgagatgga gtacgaggtg tgatggtatc ggccatttta cccaatgctg cgtaacgcct
     1741 tataacttca gcaatgaagc gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt
     1801 ccggctgtaa atcgccgaat cgtttcctgt aggccggggc agcccacatg gatgtgggct
     1861 gagggcgcgt cttacaggac gttacctcgc gcccgtaccc gatagccgaa aggaataaga
     1921 tgagggaacc gcgccagcgg cgatttaccg ccgggagccc gggttgccag ggtggtggcg
     1981 attgagccac cctggcacgt tcaccggctt gagcgttcca tagtagcgag gaacatcaag
     2041 tgaacggaac agccttgaat cttacatatt cagaatataa tggtgccgcc gcgaacgcct
     2101 gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag gtcacctggt aatctacatc
     2161 ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg ctcggcccag
     2221 tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc gcagcagctg gtccacttgc
     2281 aggctgtaat tgagttgctg ccagttgcca taaacccgca gcggtactgg cgtttctttg
     2341 aggaaatcaa tcagtttgct ttccccgttc cagccaccca cgacccgaat atcaaactgg
     2401 gtgtcgcagg tttgatctgc cagattcaac ataccttccc ctgtcagcgc cagcacaggc
     2461 gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaatgtcaa atcggtggta
     2521 aagcggtcaa gccgcgtcac gttatcgaag ttttcagcgg ccttcacatc accaccatta
     2581 cgttctaccg cttgctgaat catctgctgg aagttcatcc cttccatgcg cgtgtcggtc
     2641 atttcgacat gtgcctgtcc ttgccagtta tggcggaatg cgtcggcatc tatgtcagca
     2701 ccggagaaat caccagccag tgacatcttt ccggtcaacg aaatcggata gttaaacgcc
     2761 ttcagaatgg taccaatctc aacgttttcc agccgtggct ggaagtttat ccgcggattt
     2821 attgatgtcg cgtccagcgt gcccggcagt gaaacctttc caccgttaag cttgccctgc
     2881 agttgagtaa tttccagcaa acccgacttg ttggtcattt gcgtggcaac atatgtaaaa
     2941 ttcattccgc gccagcgcac gttactggct tgcaacaaaa tatcagccgt aaagccttgc
     3001 agtccctgat aggccggttc atcaatacgc gaagaaataa ccgggcgcgg caacgttgat
     3061 tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg cggtttcatt aagcgggatg
     3121 aggttgtcaa gattcagttg cgggaattgc agtctcagct gccattcagg tttctctgtc
     3181 agcgtgacct gtgcttgccc gctcagcgtg ctgtcattgg cagtcaagct gatttggtta
     3241 aatgaaaggc gtttatgtga ctcctgccac tgggcctgga aactcccctg cccctgaata
     3301 ccttgttttg gtaaatctgc accctgcaac tgccagttaa tttgttcaat agccgccgtt
     3361 aaatcatgcg gataatcaga agcatctacc gtaccgttaa gggatatcgt gagatcgcgc
     3421 tgatcgcgat taacccgccc ggagaactca aatgagccac gatgttgggg atcttgttcc
     3481 atttgcaggc ggatattgcg gattgtcacc tgctcgtcat cttcatgctg gaacaccagc
     3541 acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc gtctgacaga
     3601 tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc
     3661 tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct cagttgatga
     3721 ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg
     3781 cttgcgccct gggcggtgag agacattcgc ccggagagga tactaagctg cggccagacg
     3841 tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc aacttgcttg
     3901 accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga taacccggcc
     3961 accagcacga ccaggagtat catcagcgtc gtcagaaatc gtctcatggt atcctcaatg
     4021 ggcctgaatt agtctttatc gattcggctg gctaccgcgc cctgctggtt gcgatatttc
     4081 gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc
     4141 gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt gtagaactcc
     4201 agcacaatgc aaccagacca gcccggatcg atacggtgcg cggtgacgtg caccatcagc
     4261 cccagacgcg ccaaagagga acgcccgtcc agccagccca ccagatcggc tggcagcgtc
     4321 accgactcca gcgtcaccgc cagcgccagc tctcctgggt gaagatagaa cgcctcgccc
     4381 tcgtcgagaa cgatctcatc gctcatcacg cggtcaagcg cggcgctcac ttcatctttg
     4441 ggaccgctca gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa tttattgccc
     4501 aggcgtacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg gttgatcgac
     4561 aaacggcctt catcaagcca ggcttcaata tctcggtcac acagacgcat ggcattttct
     4621 cctttcgcgc atcactccct taacgccaat tacgtcaagg gcataccagg ttatcactga
     4681 acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt tcaatatatc
     4741 gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt gtttagaagg
     4801 ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga tattgcgcca tcacggaatc
     4861 cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc agatatccag
     4921 cggggtatca acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa
     4981 caaaatgcct tcgagaatga tgaccttctt cggctcaacc gtcaccgttt ctttcatacg
     5041 cgtatgttca acatagctgt aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg
     5101 taaatgctca agcaacaggc tgtgatccat cgcgctggga tggtcgtagt tggtcttaac
     5161 gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg gaattacgcc
     5221 gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg caataagact
     5281 cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag actgatcagt
     5341 catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa gcgccaaacg
     5401 cggcaattat agggatttca tccgcctgat accagtcgaa tagcgctgcc gcgcgctcag
     5461 agttaattgt tgacgaagaa ttcccggtgg caaattacgt tgatcagttt tatacaaggt
     5521 aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta tgagcaattc
     5581 gcatataaaa tgtaaaactt ttgtactagc ataaacacag aaacgaatac tggcgacctg
     5641 gtcttgcgga taaagcggta atgagcaaac aatcacagca tgtattaatt gccctgcccc
     5701 acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatctttacc cttttctcgc
     5761 ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg tccatcatga
     5821 tggtggcgtt ttatcgccat gccgggcgca tgtggccggg aattgcgctg agctgctcgc
     5881 tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac atgacctgga
     5941 cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt
     6001 ggtataaccc cttgcaaaat ctggctgact ggctgcgtct ggcattcggc agcgccattg
     6061 ttccgcctct gctagggggt gttctggttg tcctgctgac gcccggagac gatcctctca
     6121 gggcattttt gatatgggta ctgtcagaat ccatcggcgc tctggcactg gtgccgctgg
     6181 gattgttatt taaaccacac tatctgctgc gccatcgcaa cccacggttg ctttttgagt
     6241 cgctgctcac gttagccatc acactgacgt taagctggct ttcgatgctg tatctgccgt
     6301 ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg
     6361 aagccttttt gatcttcctt accacggtga tgatggtgtc gctgatgatg gccgcggatc
     6421 cctccctgct tgctacgccg cgtacatacc tgatgagcca tatgccgtgg ctaccgtttt
     6481 tgctgatcct gctgcccgcc aacatcatga cgatggtgat gtatgccttt cgtgcggaac
     6541 gcaaacacat ttccgaaagc gaaacccgtt ttcggaacgc catggaatat tccgctatcg
     6601 gtatggcgtt agtgggcacc gagggacaat ggctgcaaac caacaaagcg ctctgccagt
     6661 ttctcgggta cagtcaggaa gagctgcgcg gactcacctt tcagcaactg acctggccgg
     6721 aggatctcaa taaagatctc caacaggttg aaaagctgat aagcggtgaa ataaacacct
     6781 attcaatgga aaaacgctac tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg
     6841 tctcactggt gcgccacacg gatggcacgc cgctctattt tatcgctcag attgaagaca
     6901 ttaacgagct aaaacgcacc gaacaggtga atcagcaact gatggagcgc atcacgctgg
     6961 ctaacgaagc gggcgggatt ggcatctggg agtgggagtt gaagccgaat atttttagct
     7021 gggataagcg gatgttcgag ctgtatgaaa ttcctccaca tatcaaaccg aactggcagg
     7081 tgtggtacga gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt
     7141 cgttgcaatc acgctcgccc tttaaactgg aatttcgcat taccgtaaaa gacggtattc
     7201 gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc
     7261 tcggcattaa tatggatatg actgaggtta aacagcttaa cgaggcattg tttcaggaaa
     7321 aagagcgcct gcacataacg ctggactcca tcggcgaagc cgtggtctgt attgatatgg
     7381 caatgaaaat tacctttatg aatccagtgg cggagaagat gagcggctgg acgcaggaag
     7441 aagcattagg tgttccgctc ctgacggtgt tgcatattac ctttggcgac aatggtcccc
     7501 tgatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa gatgtggtgt
     7561 tgcactgccg gagcggcggc agctacgacg tgcattacag tattacgccg ttaagtactc
     7621 tggacggcag caatattggt tcggttctgg tgattcagga cgttaccgaa tcacgcaaaa
     7681 tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc gccaatcgcg
     7741 ccagttttga gaagcaactg cgtatgctgc tgcaaacggt aaacagtaca catcagcgac
     7801 atgccctggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc gccgggcatg
     7861 cggcgggcga cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg ctgcgttcca
     7921 gcgacgtgct ggcgcgactc ggtggggatg aatttggtct gctgctacca gactgtaatg
     7981 ttgaaagcgc gcgttttatc gctacacgca ttatcagtgc cgtgaatgac tatcacttta
     8041 tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg gattaccttg attgatgaca
     8101 acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa
     8161 atggtggacg gggccgggtg acggtttacg aaccacagca agctgccgca catagcgaac
     8221 gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag ttgatgatga
     8281 tggcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg ctgatttcgc
     8341 ttaaactctg gagttgcgaa ggcgagatta ttgatgaaca aaaatttcgt cgtagcttca
     8401 gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgaattt ttccagctgg
     8461 ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct ccccctttcc gttgccggtt
     8521 tgagtagcgc cacgctggtg aatgatctgc ttgagcagct ggaaaatagc cctctaccac
     8581 cacggttatt acatctgatt attccggcag acgcgatttt agatcacgca gaaagcgtgc
     8641 aaaaactgcg gctggcggga tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa
     8701 tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt gagttatgcg
     8761 ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag gggcacgctc
     8821 agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg atggatacgc
     8881 tttctggcat cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg
     8941 atttgctggt gaatagcagt tatttcgcga ttaactgagg gagcgtttgc ccattgcctg
     9001 atgcgacgca tcaggcctac aattcgtccg ttccgtctgg ttgccagcct tccgtatacc
     9061 agatatgcaa cagcgcataa gaacgccagg gcttccagcg ctcggcataa cggcggattt
     9121 gtgccggtgt catccccgga aatcgctgtt taatcagata atcatccggc agaaaaacat
     9181 ctttcgcctg ccagccacgc aaagcgaaat aattcgccgt ccagcgcccg atacccggaa
     9241 aagtttgcag cgttttcatc gcctgctcca catcgcccgg aattgtcatt ggtaagctgc
     9301 cctccagcgc cgcatttgcc agatgaatca gtgcctctgc ccgtttcaac ggcatgccta
     9361 acgcttttaa tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg aagcagatat
     9421 actccggaaa atcatccagc cgttcgccat aaagctgtac aactttggca gtcaattttg
     9481 ccgccatcgc cacgctcacc agttggccta aaatcgcccg cacaccctgc tcaaaagcat
     9541 caacacagcc gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc aacgcaccgt
     9601 taacaatctg cgggttacat tgcagatcaa acaggcggct cattttcgcc agacactcag
     9661 cggcaacggg ttctaaacct gcacttaaat ttatgtgcag agtatggcgg gctatatccg
     9721 gaatagcagt caccacgccg cgatattcgc ccaccgccag actacgggca taataatcct
     9781 cagcgaccgt ttccacacca ctcactgcac gggcggcgag aaatcccaac atccacgacc
     9841 agtcatacgg cggctgccag ttcagggtat acatcgcatc tccttattca tccgctttca
     9901 gcataaacgt tattcagacg ctacgctttg ctttcatatt ccggttgtcg cgacagcaac
     9961 atttcgctaa agtcacgccc cttcttcacc ggcatgggga ttaattcgtg tttattggtt
    10021 ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa ccgcaattac
    10081 tgaaaatgga aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaat
    10141 cggtaagcga atggctgtac cgccatcatg atgttccggc agacgacgat gaaacgcagg
    10201 cgctgttacg tcgggcgatt cgttataacc gcgaagaaga tatcgatgtt acggcgaaaa
    10261 gcgtgcagtt cggtctttcc tcactggcac agtacattga tgatccagaa gaagtgtggt
    10321 ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc
    10381 tgtttgagga tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc
    10441 tgccagaagc gattactcag gcggtgattg gtcgcccgat caacttccag gggctgggcg
    10501 gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag cgtgccggat
    10561 tcagggacgt ggtattccag tacgagccgg tcgcggctgg gctggattac gaagccacct
    10621 tgcaggaaga aaaacgggtg ctggtggtgg atatcggcgg tggtacgact gactgttcat
    10681 tgctgctgat ggggccgcag tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc
    10741 acagtggttg ccgtattggc ggtaacgatc tggacatcgc actggcgttt aaaaacctga
    10801 tgccgttact gggcatgggt ggcgaaaccg aaaaaggcat cgccctgccg attctgccgt
    10861 ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag tgatttctac agtagtgcca
    10921 acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa gtggccctgc
    10981 tacagaaagt ctggcgtcag cgtttaagct atcgcctggt gcgtagcgca gaagagtgca
    11041 aaattgctct ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc agcgatgaac
    11101 tggctacgct gattagccag caagggctgg aaagcgccct cagccagcca ctggcgcgga
    11161 ttctggaaca ggtgcaactg gccctggata acgcccagga aaaaccggac gttatctacc
    11221 tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaagc gctggcagaa cagttgccgg
    11281 gaattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg gcacgctggg
    11341 cggaagtggt gtttcgttaa aagaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    11401 gagccagttt ttctgccgcc tgacgtaaag gttccatcaa cggcatggtc cgtgctggag
    11461 catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgtcgat tccagccgat
    11521 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    11581 tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc gcctgggtca
    11641 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    11701 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    11761 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    11821 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    11881 cgagcatcgc ccgtttggcg ataaaacctt ccagcccgcc caactgttgg atctggctga
    11941 ctaaatgctc atagtccatt ttcgcgcgat taaatgcctc acggcgttgc tggatttctg
    12001 ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgacagc acaggaacca
    12061 aaataatata acgcggttct gcctgaaggc cgcaaagcaa cagcgataac gctgacagtg
    12121 cgatctcaat cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    12181 tcggttggaa atgatcgggt aatggcaatt gcaatgctgg cggtgctact gacgccatca
    12241 ccatcgccca gactttcgcc agcacaaaat caccgccggt ggtaatgacc tcttcgccga
    12301 gatcaataaa atagataacg ccttgattat ccagcgcaca ccacgggcag tcggtcaaat
    12361 gagcgggata aacatgcatt gccgaaacgg gacatttctt taactgttgg cgcagagaat
    12421 caagtgccgc tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtga
    12481 atgcctgctg aaacatggct tcaacatcgc ccggtaacat cgatagcgga atcgatcgtg
    12541 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    12601 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    12661 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    12721 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    12781 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    12841 tcagtaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    12901 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    12961 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    13021 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    13081 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    13141 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    13201 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    13261 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    13321 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    13381 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    13441 attgaaaaac ggggtaaaga aaggtacatg aggagaatta tccagcatat ttaacgccag
    13501 tcgctggata ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc tggtgaaagt
    13561 ttccagtctg gaaacggcat cttcatcggt aatgaagtgc gtcatattgg catattcacc
    13621 caccatcggc gtaagcggca attgcagacc gtgaccaaaa tccaccacca cgccgccatc
    13681 gccaatctgc ataatcaagg tgccattagc cgatgaaatc aatcccagaa acgtgcaggc
    13741 aaaatcgcga actgctaatt ctttggcttc cgcctcggca aaaagtcgct gacgaacggt
    13801 cagcacaata tccgtggcga ggatatcatt aagccccaat tccccaccct gcactttttg
    13861 cgacatataa gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    13921 gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat ttagccaggc
    13981 aacctgcatc tggcaggcat cctggcaagg caagtccgcg ctgatgtgcg atgttccaac
    14041 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    14101 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    14161 aacagttcac ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct gatttgtgcc
    14221 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aagcaaacct tttatcttct
    14281 tccccccgaa acactttgtt ggctgcagcc tgccactcat cggttggtgc accatcggta
    14341 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc acgtttccgc
    14401 tcctctacca tatccagggc tttggtaatg gccgcaccca ttggtgtatc gccctgggca
    14461 aacaggatgg gcggaaaaaa attagcggcg ctggtaaagg gttgttccac atgcaccggg
    14521 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcggc aagcagttca
    14581 tcacgaaagg tcaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    14641 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    14701 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    14761 gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    14821 cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    14881 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    14941 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    15001 catcataacc ctttccttat taaaagccct cttctccggg agaggctttc ccgtttcagc
    15061 gtcccgctga aatcatcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    15121 tgaataaaat agtttcctct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    15181 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca tactttcagg
    15241 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    15301 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct
    15361 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc
    15421 gccatgcact tagtctatcg ctgacttgca gataaaatag tttccagtgt gattattgcg
    15481 taacgtctcg caaagaattt ttgctggcag gatcgcagac ggcaaagcct gcggattgac
    15541 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
    15601 gtagtcttag tatgctgggt agctaatcac taagagcacc acgatgatga gtagcttcat
    15661 catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcatcc
    15721 ggctgaaatc tgcggcttac ctcctttcgc cctgtatgca gtctatcgct aacgcgtaga
    15781 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac
    15841 gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
    15901 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
    15961 cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
    16021 aaggccgcaa tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc
    16081 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
    16141 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattacc gccgctaata
    16201 ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc
    16261 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
    16321 tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg
    16381 atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg
    16441 cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
    16501 ccagcgcgca gctgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg
    16561 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc gggatcgtgg
    16621 tgatcaccca gacgcatcct atcgatttgc tctttaccct gccggaaagc gatatcgcta
    16681 ccgtcgtgca ggcgcagaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca
    16741 actcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa
    16801 ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc
    16861 agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacgg
    16921 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgagtagc gaaaacaagg
    16981 tcagcaaaca tctagtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag
    17041 gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga
    17101 aagtggaagt ggtggaagcc cagagcgcca ctactccgga agagaaagcc accagccgcg
    17161 aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
    17221 cccgtcgcgc ctgtttatta tgcgtcctgt ggccaccacg ctgctgatgg tggcgatctt
    17281 actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta
    17341 tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc
    17401 cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc
    17461 gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga
    17521 tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga
    17581 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
    17641 cgccgtcacc tcaaccgcca tgccgatgac ccaggtagaa gatatggtgg aaacccgcgt
    17701 cgcgcagaaa atctcgcaga tttccggtgt cggactggtg acgctttccg gcggtcagcg
    17761 tccggctgtt cgcgtcaaac ttaacgccca ggcgattgcc gccctcggcc tgaccagcga
    17821 aaccgtgcgc accgccatta ccggagctaa cgtcaactcg gcaaaaggta gcctcgacgg
    17881 cccttcccgt gcggtcacgc tttccgctaa cgaccagatg caatccgccg aagagtatcg
    17941 ccagctaatc atcgcctacc agaacggcgc accgattcgt ctgggcgatg tcgcaaccgt
    18001 agagcaagga gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt
    18061 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcatccggca
    18121 gatgctgcca cagctcaccg agagtctgcc gaaatcggtg aaggtgacag tgctttccga
    18181 tcgcaccacc aatattcgcg catccgtcga tgatactcag tttgaattga tgatggctat
    18241 cgcgctggta gtcatgatca tctacctgtt tttgcgcaat attccggcga ccatcattcc
    18301 cggtgttgct gtaccgctgt cgttaatcgg cacttttgcg gttatggtgt ttctcgattt
    18361 ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga
    18421 cgccatcgta gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc
    18481 ggcggcgctc aaaggcgcgg gtgaaatcgg ctttaccatt atctcgctga ccttctcgct
    18541 gattgcggtg ttgattccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga
    18601 atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac
    18661 accgatgatg tgcgcgcgga tgctcagcca ggagtcgctg cgtaaacaga accgattctc
    18721 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa
    18781 ggtgctgaat catccgtggc tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt
    18841 tctcttatgg gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca
    18901 gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca
    18961 ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt
    19021 tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgctgga
    19081 tgaacgtgat gaccgagtgc aaaaagtcat tgcccgtctg caaacggcgg tggataaagt
    19141 gccgggcgtc gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag
    19201 ccgcacccag taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt
    19261 gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg
    19321 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
    19381 gatcagcatg gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc
    19441 cactatttat actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac
    19501 cccgggcctc gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc
    19561 gttaagctca attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga
    19621 tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc
    19681 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
    19741 gttccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
    19801 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
    19861 gatcaccatt ctctcgacgc tacccaccgc aggggttggc gcactgctgg cgttgctgat
    19921 tgctggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt
    19981 gaagaagaac gccatcatga tgatcgactt cgcgctggct gccgagcgcg agcaaggcat
    20041 gtcgccgcgc gaggcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
    20101 cactctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
    20161 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
    20221 gctgtttacc acgccggtga tttatttgct gttcgaccgc ctggcattgt ggaccaaaag
    20281 ctgctttgcc cgtcatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
    20341 cccggtggcg acgattttac tgtcggttgc cattaccctg tgcggcatac tgggcttccg
    20401 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
    20461 gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc
    20521 acttgggcgc attgccggag tcagtgaaat gacctccagc agttcgctcg gcagcacgcg
    20581 tattattttg cagtttgatt ttgaccggga tatcaacggc gcggcgcgcg atgtgcaggc
    20641 ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgtc cgacctatcg
    20701 caaagctaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
    20761 gcagggtgaa ctgtacgatt ttgcctcgac gcagctggct ccgacgattt cgcaaatcga
    20821 cggtgttggt gatgtcgatg tcggcggcag ctcactgccc gccgtacgcg tcgggctgaa
    20881 tccgcaggcg ctgtttaatc agggcgtctc gctggacgac gtacgcactg ccatcagcaa
    20941 tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca
    21001 gaccaatgat gagctaaaaa ccgccgacga atatcagccg ctgattattc attacaacaa
    21061 cggcggcgcg gttcgtctgg gcgatgtggc gacagtgacc gactctgtac aggatgtacg
    21121 taacgccggg atgaccaatg ccaaaccggc tattttgctg atgatccgca agctgccgga
    21181 agccaatatt atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac
    21241 cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgctcctc
    21301 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtatt
    21361 tttattcctg cgctcgggtc gcgccactat tattcccgcc gtttcggtgc cggtttcgct
    21421 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat
    21481 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
    21541 tgcccgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga
    21601 agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
    21661 gttgatgggc ggattgccgg gccgactgtt acgcgaattt gccgtgacgc tttctgtcgc
    21721 cattggtata tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct
    21781 gaaagccagc aagccgcgcg agcaaaagcg aatgcgtggt tttggtcgca tgttggtagc
    21841 cctgcaacaa ggctacggca agtcactgaa atgggtgctc aatcataccc gtctggtggg
    21901 cgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac
    21961 cttcttcccg gagcaggaca caggcgtgtt gatgggcggt attcaggcgg atcagagtat
    22021 ttcgtttcag gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc
    22081 ggcagtggat aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt
    22141 tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
    22201 gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
    22261 tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct
    22321 ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact
    22381 ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg
    22441 tgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt taaataacgc
    22501 cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
    22561 ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa
    22621 taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc
    22681 actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttta acctgccgac
    22741 cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
    22801 gccttcgacg gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa
    22861 ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
    22921 ttacgagagt tatgtgcacc cgctgacgat tctctccacc ctgccctcgg cgggtgttgg
    22981 agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat
    23041 catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga
    23101 agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg
    23161 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt
    23221 gtcgggcggt gacggctcgg agctgcggca acccctgggg atcaccattg tcggcggtct
    23281 ggtaatgagc cagcttctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg
    23341 tctgcggctg cgtttttcgc gtaaacccaa acaaacggta accgagtaaa tgacagatct
    23401 tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
    23461 ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc
    23521 gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc
    23581 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct
    23641 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
    23701 acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat tgacggtgat
    23761 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca
    23821 ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca
    23881 ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgatgtt
    23941 aatgccgaac tataccatgc agacgcggcg cttcgatctc tccggctttt tattgctggc
    24001 ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc
    24061 gctgacgatt gctggcctgg tcgcagttgg cgtggtagca ctggtgcttt atttgctgca
    24121 cgccagaaat aacaaccgcg ctcttttcag tctgaaactg ttccgcactc gtaccttttc
    24181 gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac
    24241 accggttttc ctgcaaattg gcctcggttt ctcaccgttt catgccggac tgatgatgat
    24301 cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gtacaggtgg tgaatcgctt
    24361 tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt
    24421 tatgactacc gccctgctgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
    24481 gatggtcaac tcgacgcgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
    24541 caatctggcg agcagcggca acagcctgct gtcgatgatt atgcaattgt cgatgagtat
    24601 cggcgtcact atcgccgggc tgttgctggg actttttggt tcacagcatg tcagcgtcga
    24661 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt tgatcatcgc
    24721 ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc
    24781 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt
    24841 ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc
    24901 agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg
    24961 ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac
    25021 aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa
    25081 cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac
    25141 aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt
    25201 ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc
    25261 aatactgata tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgctctg
    25321 gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gcggtttact ggcaccggta
    25381 aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg
    25441 cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcaca
    25501 ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt
    25561 acgccattag cggtgctgcg cggtgaactg gaagccattc aggatggcgt gcgtaaattc
    25621 acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac
    25681 gatctccatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta
    25741 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagtcgt
    25801 ggcctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt
    25861 ttaatgcagt tattcaataa cttactggaa aacagcctgc gctacactga cagcggcggc
    25921 agcctgcaaa tctctgccgg gcagcgcgac aaaacggtgc gcctgacctt tgccgacagt
    25981 gcgccaggtg tcagtgacga tcagctacaa aaattgtttg aacgttttta tcgcaccgaa
    26041 ggttcccgca accgtgccag cggcggttcc gggctggggc tggcgatttg cctgaacatt
    26101 gttgaagcac ataatggtcg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    26161 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    26221 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca
    26281 ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    26341 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca
    26401 ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg
    26461 tgacggcaaa aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt
    26521 atatctgtaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc
    26581 gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg
    26641 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacacct gcggaatttc
    26701 gtctgttgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca
    26761 atcatcttta tgacgactac cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa
    26821 acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg
    26881 gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacattatt
    26941 ttgttagccg gagatggcgt tcagacccga tccggcaaca ttatcccacg catggtcagc
    27001 aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt
    27061 aaacagcacc tttagatgct gttttcgata cacaatttca atcaaggagt cattatggct
    27121 ggttggtttg aactcagtga gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc
    27181 aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc
    27241 atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt
    27301 aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag
    27361 atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca
    27421 agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg
    27481 ctttatatcc aaataacgct tagttattaa taattaagag taaccgccgc cattaaaata
    27541 gtgggcactg acgagcactc gttgtgcttt ctttgtttac catcccatac ttatcctcct
    27601 gaacatgttc atgtgccaaa atatcttcta atactttcgt gacgctatcc ggtttatgac
    27661 atacactgat gtaagatttt tacttacagg cgggcgtaaa atggccagaa cgatgacggt
    27721 tgatctcggc gatgagttgc gtgaatttat cgagtcctta attgaatccg gcgactatcg
    27781 cacccaaagc gaagtgatcc gtgagtcact gcgattactt cgagaaaagc aagcagagtc
    27841 tcgcctgcaa gcgttacgtg acatgctcgc agaagggttg agtagtggtg aagctcagcc
    27901 gtgggaaaag gacgcgttct tacggaaggt gaaagcaggg ataaggaaat aatgtgcgta
    27961 ttattaaact catgccaaaa gccaatgagg atttagaagg catctggtat tacagctatc
    28021 accatttcgg cgaaccacaa gccgacagat atgtggaaca tctttcagac gttctccaga
    28081 ttctgagtaa caataatata ggtacgccca gacctgaatt aggcgaaggt atttttgttc
    28141 ttccttttga acgccacgtc atttatttcc tgcaatcacc aggggaaatt atcgtcattc
    28201 gtatccttaa ccaaaaccag gacgccacca gacatttaca ctggagctaa acccgccgat
    28261 tgcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca tcaggtgaag
    28321 cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg
    28381 cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg
    28441 gccagccgcg ttattccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc
    28501 tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac
    28561 cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc
    28621 cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg
    28681 aaatgccgat ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg gtgaaattct
    28741 ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg
    28801 aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcgctgt
    28861 gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc gacccgaacc
    28921 agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg
    28981 atgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc
    29041 tgggtatcgg cgcaccaacc gacaaagtgt ttatgatcga agaggcccag cgtccgggcg
    29101 agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg aaagatctgc
    29161 gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag
    29221 gtcgtaccaa atctttctac tattgtgcac gcaccgcaca ggtttaccgc aaagctatcg
    29281 atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctgg
    29341 cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact
    29401 acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc
    29461 gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg
    29521 agctgatgac gccgcaaggc aacattaatt ttacccttga gcacatggaa aacgccaaag
    29581 gcgaagctat gccgatagca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg
    29641 atcttgagct caattacgcg ctgctgatgc gtaatttctc cggggaaacc acgcgtaatc
    29701 cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat
    29761 cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa
    29821 atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat
    29881 tttttatgca taagccctat ccctggtcac cgtcttccat tgaccacatc gatagaatct
    29941 cccttcatag cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag
    30001 cacaaccaga acgcgcatgt ttgacgctta ccaaaaaata ttctcactct ccacatttga
    30061 atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca
    30121 tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac
    30181 cagatattct gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt
    30241 tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat
    30301 gcacagcaca accagacata ataaaaatac taattaatga tatagcagct atctttttca
    30361 tctcaccttc ccccattaa
//
LOCUS       DAZGCS010000057        30242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGCS010000057 DAZGCS010000000
VERSION     DAZGCS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.057"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..471
                     /locus_tag="ACHK01_002701"
     CDS             <1..471
                     /locus_tag="ACHK01_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO8097631.1"
                     /translation="VGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGI
                     KREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTG
                     TIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            701..1084
                     /gene="secE"
                     /locus_tag="ACHK01_002702"
     CDS             701..1084
                     /gene="secE"
                     /locus_tag="ACHK01_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO8097632.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1086..1631
                     /gene="nusG"
                     /locus_tag="ACHK01_002703"
     CDS             1086..1631
                     /gene="nusG"
                     /locus_tag="ACHK01_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO8097633.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1790..2218
                     /gene="rplK"
                     /locus_tag="ACHK01_002704"
     CDS             1790..2218
                     /gene="rplK"
                     /locus_tag="ACHK01_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO8097634.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2222..2926
                     /gene="rplA"
                     /locus_tag="ACHK01_002705"
     CDS             2222..2926
                     /gene="rplA"
                     /locus_tag="ACHK01_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO8097635.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3339..3836
                     /gene="rplJ"
                     /locus_tag="ACHK01_002706"
     CDS             3339..3836
                     /gene="rplJ"
                     /locus_tag="ACHK01_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO8097636.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3903..4268
                     /gene="rplL"
                     /locus_tag="ACHK01_002707"
     CDS             3903..4268
                     /gene="rplL"
                     /locus_tag="ACHK01_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO8097637.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4588..8616
                     /gene="rpoB"
                     /locus_tag="ACHK01_002708"
     CDS             4588..8616
                     /gene="rpoB"
                     /locus_tag="ACHK01_002708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO8097638.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8693..12916
                     /gene="rpoC"
                     /locus_tag="ACHK01_002709"
     CDS             8693..12916
                     /gene="rpoC"
                     /locus_tag="ACHK01_002709"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO8097639.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13157..14290)
                     /gene="thiH"
                     /locus_tag="ACHK01_002710"
     CDS             complement(13157..14290)
                     /gene="thiH"
                     /locus_tag="ACHK01_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO8097640.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(14287..15057)
                     /gene="thiG"
                     /locus_tag="ACHK01_002711"
     CDS             complement(14287..15057)
                     /gene="thiG"
                     /locus_tag="ACHK01_002711"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO8097641.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15059..15259)
                     /gene="thiS"
                     /locus_tag="ACHK01_002712"
     CDS             complement(15059..15259)
                     /gene="thiS"
                     /locus_tag="ACHK01_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO8097642.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15243..15998)
                     /gene="thiF"
                     /locus_tag="ACHK01_002713"
     CDS             complement(15243..15998)
                     /gene="thiF"
                     /locus_tag="ACHK01_002713"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO8097643.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15991..16626)
                     /gene="thiE"
                     /locus_tag="ACHK01_002714"
     CDS             complement(15991..16626)
                     /gene="thiE"
                     /locus_tag="ACHK01_002714"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO8097644.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16626..18521)
                     /gene="thiC"
                     /locus_tag="ACHK01_002715"
     CDS             complement(16626..18521)
                     /gene="thiC"
                     /locus_tag="ACHK01_002715"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO8097645.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18602..18702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18754..19230)
                     /gene="rsd"
                     /locus_tag="ACHK01_002716"
     CDS             complement(18754..19230)
                     /gene="rsd"
                     /locus_tag="ACHK01_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO8097646.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19325..20098
                     /gene="nudC"
                     /locus_tag="ACHK01_002717"
     CDS             19325..20098
                     /gene="nudC"
                     /locus_tag="ACHK01_002717"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO8097647.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20138..21202
                     /gene="hemE"
                     /locus_tag="ACHK01_002718"
     CDS             20138..21202
                     /gene="hemE"
                     /locus_tag="ACHK01_002718"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO8097648.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21212..21883
                     /gene="nfi"
                     /locus_tag="ACHK01_002719"
     CDS             21212..21883
                     /gene="nfi"
                     /locus_tag="ACHK01_002719"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO8097649.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYILGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21926..22516
                     /gene="yjaG"
                     /locus_tag="ACHK01_002720"
     CDS             21926..22516
                     /gene="yjaG"
                     /locus_tag="ACHK01_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO8097650.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22703..22975
                     /gene="hupA"
                     /locus_tag="ACHK01_002721"
     CDS             22703..22975
                     /gene="hupA"
                     /locus_tag="ACHK01_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO8097651.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22988..23683
                     /gene="yjaH"
                     /locus_tag="ACHK01_002722"
     CDS             22988..23683
                     /gene="yjaH"
                     /locus_tag="ACHK01_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO8097652.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23685..24110)
                     /gene="zraP"
                     /locus_tag="ACHK01_002723"
     CDS             complement(23685..24110)
                     /gene="zraP"
                     /locus_tag="ACHK01_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO8097653.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            24348..25724
                     /gene="zraS"
                     /locus_tag="ACHK01_002724"
     CDS             24348..25724
                     /gene="zraS"
                     /locus_tag="ACHK01_002724"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO8097654.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25721..27046
                     /gene="zraR"
                     /locus_tag="ACHK01_002725"
     CDS             25721..27046
                     /gene="zraR"
                     /locus_tag="ACHK01_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO8097655.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27043..28332)
                     /gene="purD"
                     /locus_tag="ACHK01_002726"
     CDS             complement(27043..28332)
                     /gene="purD"
                     /locus_tag="ACHK01_002726"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO8097656.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28344..29933)
                     /gene="purH"
                     /locus_tag="ACHK01_002727"
     CDS             complement(28344..29933)
                     /gene="purH"
                     /locus_tag="ACHK01_002727"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO8097657.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7085 a         7869 c         8334 g         6954 t
ORIGIN      
        1 gttggtgaag aagttgaaat cgttggtatc aaagagactc agaagtctac ctgtactggc
       61 gttgaaatgt tccgcaaact gctggacgaa ggccgtgctg gtgagaacgt aggtgttctg
      121 ctgcgtggta tcaaacgtga agaaatcgaa cgtggtcagg tactggctaa gccgggcacc
      181 atcaagccgc acaccaagtt cgaatctgaa gtgtacattc tgtccaaaga tgaaggcggt
      241 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
      301 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      361 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      421 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      481 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      541 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      601 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      661 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      721 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      781 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      841 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      901 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      961 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
     1021 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
     1081 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
     1141 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
     1201 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1261 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1321 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1381 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1441 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1501 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1561 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1621 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1681 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1741 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1801 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1861 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1921 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1981 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     2041 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     2101 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     2161 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     2221 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2281 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2341 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2401 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2461 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2521 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2581 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2641 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2701 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2761 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2821 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2881 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2941 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     3001 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     3061 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     3121 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     3181 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3241 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3301 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3361 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3421 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3481 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3541 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3601 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3661 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3721 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3781 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3841 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3901 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3961 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     4021 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     4081 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     4141 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     4201 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4261 ttaaataagc caacccttcc cgttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4321 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4381 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4441 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4501 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4561 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4621 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4681 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4741 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4801 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4861 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4921 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4981 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     5041 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     5101 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     5161 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     5221 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5281 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5341 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5401 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5461 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5521 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5581 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5641 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5701 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5761 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5821 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5881 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5941 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     6001 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     6061 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     6121 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     6181 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6241 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6301 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6361 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6421 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6481 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6541 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6601 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6661 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6721 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6781 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6841 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6901 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6961 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     7021 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     7081 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     7141 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     7201 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7261 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7321 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7381 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7441 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7501 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7561 ctgccgcgcg atcgctggct ggagctaggc ctgacagacg aagagaaaca aaatcagctg
     7621 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7681 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7741 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7801 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7861 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7921 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7981 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     8041 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     8101 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     8161 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     8221 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8281 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8341 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8401 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8461 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8521 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8581 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8641 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8701 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8761 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8821 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8881 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8941 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     9001 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     9061 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     9121 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     9181 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9241 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9301 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9361 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9421 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9481 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9541 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9601 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9661 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9721 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9781 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9841 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9901 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9961 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
    10021 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
    10081 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
    10141 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
    10201 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10261 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10321 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10381 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10441 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10501 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10561 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10621 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10681 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10741 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10801 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10861 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10921 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10981 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    11041 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    11101 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    11161 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    11221 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11281 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11341 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11401 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11461 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11521 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11581 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11641 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11701 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11761 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11821 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11881 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11941 tcagggtaac gacgttctga tccctggtac cgatatgcct gcgcagtact tcctgccggg
    12001 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    12061 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccac gcgttgcgga
    12121 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctt gctgaaatca gcggtatcgt
    12181 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12241 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12301 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12361 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12421 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12481 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12541 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12601 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12661 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12721 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12781 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12841 ggtgactgcc gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12901 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12961 tttatgggtg ttattcagag ggcaaattct tcacggaatt tttaagaaag gcagggcgaa
    13021 acgaggaaga agcttttcgc cctggacaac attcctgctg acggaactac cataaaaaaa
    13081 cagaacgctt tcaacaacct tgcgtttttc atcttccgta gcggctcgga ataaatttac
    13141 gttgttacat tctgtctcat ggcctttgcg aggcgtgtcc cagatagctg tcccagtctt
    13201 tccataccgg ctgcaaaccc tgagcggtta acgcggccgc aactgcttct ggtctgcgat
    13261 cgtcgtgcgg tgagaactgt tccagctcgg ggtgattgtc ggcatagcca cctggctgcg
    13321 ttttcgaaaa agcgctgacg ttattaattg ccagcggaat cacgcgatcg cgaaaccacg
    13381 gtgattcccg cgtggagagt gagagttcaa tctccggtgc aagcagtcgg aaggcgcaga
    13441 tggtttgcac taactggcgt tcatccataa tcgacgcagg ctcaatgccg ccagtacacg
    13501 ggcgcaggcg cggaaaggag acagagtaac ggctttgcca gtaatgctgt tgcagccata
    13561 gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg
    13621 cgccgaggcc tatcttatca atccccgcac gccccagccg atccggcgtt tccagccgcc
    13681 agaagaagtc ctgttttttg cctttcaaat gatggcgggc ataagtcgcc tcgtgatatg
    13741 tctcctgata aaccatcacg ccatccagac caagctgctt taactcggcg tattccgtct
    13801 ccgccagcgg ttgcacttcc atttgtagtg aagagaactg ttcacgcagg gcggggagat
    13861 gacgacgaaa gtaatccatc cccactttcg cctgatgttc accagtgact aatagcagat
    13921 gttcaaagcc catctcccgt atagcggcgc tttccctggc aatatccgct tcatccagcg
    13981 ttttgcgttt gatgcgatta ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa
    14041 gattggaaag ataaagcggg acgtagaaac taactgtgtt gccaaatcgc tgacgggtca
    14101 gacgctgcgc ccgttgggcc agttgttcca gatagccact ggcggctggc gataacagcg
    14161 ccatcatatc gtcgcgggtg agttgcgagg catttagcgc ccgttctacg tcagcagccg
    14221 ttttgccgtt gatacgcagg cggatgtcgt cccagtccag ttgtcgccag cgatcgctga
    14281 aggttttcat gccgatgcct ccagaaatcc agtcagcggg ctggtggcat gagcaaaatg
    14341 actgcggctg cccggtccgg actgacgtgc cagcaggcct gcttctaccg ccagacgaaa
    14401 tgccttcgcc atgttgacgg gatcgtccgc gacggcaatt gccgtattca ctaacaccgc
    14461 gtcggccccc atttccagcg cctgcgcggc atggctggga acgccgatgc cagcatcgac
    14521 aaccaccggc actgtggcct gctggataat aatctccagc atggcgcggg tttccagtcc
    14581 ctgattcgag ccaatcggcg cgccgagcgg catcaccgct gcacagccga cttcttccag
    14641 acgtttacac aataccggat cggccccgca gtaaggcagc acgacaaatc cctgttgtac
    14701 cagcgtttcg gcggctttca gggtttcgat gggatcgggc aacagccagc gggcgtcagg
    14761 gtgaatctct aattttaacc agtttgtgcc taacgcttca cgagccagat gggcggcgaa
    14821 aatggcttct tccgctgttt tcgccccgga tgtatttggc agcagggtca cacccgccgc
    14881 gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag
    14941 tgtcaccagc tggctgccgg aagcgcggat cgcctccacc atcagttgtg aagaagcgaa
    15001 tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac gtaacatttc
    15061 aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg atatgttgcg
    15121 cccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc gcttgtcgtt
    15181 ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc ggcgcactgc atcggttgat
    15241 cgttaaacag gatctgcatt gcttcctccg cataccgggc aaccactggc gcggcgcaac
    15301 gccaggctgc gccactggct cgatttaccg tcgaacagtc ggagttctcc cgcaggtgtc
    15361 tctataccgc ttaataactt aatggcttcc agtgcctgca aagtgcccat aaccccgacc
    15421 accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gctcctggtt atctggccac
    15481 aggcagcggt aacacccctg ctcccagggc ggcgtcagta ccatcaactg accgccaaat
    15541 ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca cgcaggcggc attaatctcc
    15601 tggcgagtcg ccatattgtc ggtacagtcg agcaccacat cggcccgtgc aaccgcatct
    15661 tttaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatgtc gggattcaac
    15721 tgtgtcagtc gctgttggct gacctgcgat ttcgggctgt tgatatcttc agtggtaaag
    15781 aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag taccagcgtc
    15841 ccgacgccag cgcccgccag gtacagcgca gcaggtgtac ccagcccgcc cagaccgata
    15901 atcagcacct ggctgtcgag cagtttttgc tgcccgtcca gagcgatatc gtcgagcagg
    15961 atttggcggc tataacgcat aaagtcacgg tcattcatcg ccaactcctg caatttccag
    16021 caactgtgcc gttgccaaac gccagtctgc agcttgagta atggcgctga cgacggcgat
    16081 actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc
    16141 cacggtggga taatccgcca gtcgctcaac atgccgtgcc agctgttcca gcccctgcgg
    16201 tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga tataagaggg
    16261 gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag
    16321 gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac
    16381 gccatacgcc tgatgcttga tcgccagccg ccagtaatcg ttgataaaca atcgcgcgtt
    16441 atagcggcgg cccagcgcaa ttgccgccac gacatcggct tccacctctt catcgcgccg
    16501 atctttgatg cgtagctgga gagtacgtac gcctgcatcc aacagacgtt cgatccactg
    16561 tacgctgtcc accaccgggt acagtcctaa acgaaaaggt acaggaggaa aatcaggctg
    16621 atacatcacg cttcctcctt acgcaggtag atttctccgc ctctggcacg gaagttctcc
    16681 gacatatccg ccattcccat ttcaatagtt tgcgtggcgg cgtaatcacg cacttcctgg
    16741 ctgattttca tcgaacagaa tttcggccca cacatggagc aaaaatgggc gactttacct
    16801 gactcttgcg gcagggtttc atcgtgataa gcgcgggcgg taaacgggtc gagggccaga
    16861 ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt atcgcgaatt
    16921 tgcgcgcccg gatgcccttt cgccaggtca gcggcgtggg cggcaatctt ataggtgata
    16981 agcccctgct taacatcttc tttattaggc agacccagat gctcttttgg cgttacgtaa
    17041 cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga cgtgaagtgg
    17101 tcatagcccg gcgcaatatc ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg
    17161 cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac gtgacctggg
    17221 ccttcaatca tcacctgcac gtcatattcc caggcaattt tggtcagttc gcccagcgta
    17281 tgcagctcgg caaactgcgc ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg
    17341 tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc gcggaagtgt
    17401 tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat cgaaccgccg
    17461 cgagagacga taccggtcag gcgtttcgcg gtcatcggca catagcgcag cagtacgccc
    17521 gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac
    17581 gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc
    17641 actgtaccga tcggcaccgg gctgttacgc aaaatccact cgcgggtttc gtgaatatag
    17701 cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca taccagcttt
    17761 tccacttctt cttcgatgga agaggtgacc gccgagttgc cgatattggc gttaactttt
    17821 accaggaaat tgcgaccaat aatcatcggc tccgattccg gatgattaat gttggccggg
    17881 ataatcgcac gtccggcagc aacttcatca cggacaaatt ccgcagtgat attttccggc
    17941 agatgtgcgc caaagctcat tcccggatgc tggtggcgta aaacctcgct acggatgcgc
    18001 tcgcggccca tattctcgcg gatggcgatg aattccattt ccggcgtgat gatgccctgg
    18061 cgggcgtagt gcagttgggt gacacggcgt cctgctttgg cgcgttttgg tgttagtaca
    18121 ccgctaaaac gcagttcgtc gaggccatca tctgccagcc gcgctttagt gtaatcggaa
    18181 ctgcgcacgg taagttcttc ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt
    18241 gccagccctt gctgcacgtt aatggcaatc tgcggatcac catacgggcc ggaggtgtcg
    18301 tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt ctttgctacc gccaattagc
    18361 gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cggggtgtgt gccagtgata
    18421 taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc
    18481 cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc caaaaagtta
    18541 aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcagg ggcgatgcga
    18601 tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc gcggatcccg
    18661 aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccgc aaatggggca
    18721 ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc
    18781 gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc
    18841 gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag
    18901 actggaatcg tagtaatcca taatctgttg agtattggct tcgagttgcg gccaaatctt
    18961 cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact
    19021 gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc
    19081 gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac
    19141 gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg
    19201 ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac
    19261 tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag
    19321 agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca
    19381 aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt
    19441 gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca
    19501 gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt
    19561 tcaactggcc ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg
    19621 ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg
    19681 ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga
    19741 ttcgatcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg
    19801 attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag
    19861 cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc
    19921 tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga
    19981 attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac
    20041 cgtagcgcgc cgtctgatag aagatacggt ggcgatgtgt cgggcagagt atgagtgatg
    20101 atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg
    20161 ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca
    20221 ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc
    20281 gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc
    20341 gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg
    20401 gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga
    20461 cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt
    20521 gcgtaccatt cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc
    20581 gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa
    20641 aaaaatgatg tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag
    20701 cgtcactttg tatctgaatg cgcagattaa agccggtgct caggctgtga tgattttcga
    20761 cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgcttt attacatgca
    20821 taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt
    20881 taccaaaggc ggcggacagt ggctggaagc catggcagaa accggttgcg atgcgctggg
    20941 cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt
    21001 gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt
    21061 agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg
    21121 cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact
    21181 gtctgaacag tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca
    21241 aatcgaactg gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct
    21301 gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt
    21361 gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac
    21421 catgccttac attctaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg
    21481 ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc
    21541 tcgccgtctt ggcgtcgcca gccattttgg cttattggtg gatgtgccga ccattggcgt
    21601 ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc
    21661 cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa
    21721 cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca
    21781 acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc
    21841 ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcggg
    21901 taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatttgc gtctggagcg
    21961 cctggaaagc tggcagcacg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa
    22021 ttacgccatg ttttgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct
    22081 cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact
    22141 ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc
    22201 ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga gtggtgaaac
    22261 gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat
    22321 gactcaggct ggtcgtgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca
    22381 agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat
    22441 cgagctgata aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat
    22501 aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt
    22561 cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg
    22621 cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat
    22681 aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa
    22741 agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac
    22801 tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa
    22861 ccaccgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc
    22921 agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg
    22981 cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg
    23041 atcattgatg ctggcaggcg ctctgtttct cactgcctgt agtcacaact cttcacttcc
    23101 tccctttacc gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga
    23161 cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac
    23221 gacgcgagag tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag
    23281 caaaccgccg gtgaacattc gcgcgcgttt tgacgatcgc ggcgatctga gctttatgca
    23341 acgtgaatcc gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta
    23401 tcgtgctgat cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg
    23461 ccaggggcgc tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa
    23521 acctgattta gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt
    23581 tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa
    23641 ctctgatttc tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata
    23701 cccatatgac cgccaccacc gcagccgccg taacccatgc ccattccggt accgcgcgga
    23761 atacccgctt cagccatcgc aatatctcgt ttcacccgta actcatctaa cgactgacgc
    23821 aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct
    23881 aacagggcat tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga
    23941 gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg
    24001 gcattttgct gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc
    24061 attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct
    24121 ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg
    24181 acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca
    24241 tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga
    24301 tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc
    24361 aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg
    24421 ggctggtagg gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg
    24481 ccagacaaac gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc
    24541 gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg
    24601 ccgggcagcc tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc
    24661 atagcaactc cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac
    24721 atcctggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag cctgttctgg
    24781 aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc
    24841 agcagtatgc cgcaacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga
    24901 gtgccgaaga tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct
    24961 tgctggcaag cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc
    25021 tacaagatga aatgaagcgc aaagagaagc tggtggcgct ggggcatctt gcggcaggcg
    25081 ttgcccacga aatccgtaac ccactttcct cgattaaagg actggcgaaa tactttgccg
    25141 aacgcgcgcc agcaggggga gaagcgcatc aattggcgca ggtgatggcg aaagaagccg
    25201 accgtttaaa ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt
    25261 tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta agccaggatg
    25321 caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg
    25381 ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga
    25441 ttggtcagca tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca
    25501 gcgttaccga cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt
    25561 acttcaccac caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg
    25621 aacaacacgg tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc
    25681 tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga
    25741 tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg
    25801 ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga
    25861 acaggttttt gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac
    25921 gctgaaagag atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc
    25981 cagcgtcgag acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc
    26041 gctggatttc gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat
    26101 tgatgctgaa acacctgcgg tgactgccag ccagttcggt atggtcggta aaagctcggc
    26161 gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cggtactgat
    26221 ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc
    26281 acgtagcgaa aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga
    26341 atctgaattg ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg
    26401 gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc
    26461 gatgatgcag gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag
    26521 caaccagact atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc
    26581 agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat
    26641 tgaagtacca tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct
    26701 gcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga
    26761 tctgttgatt cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg
    26821 ggcagtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc tggcgattgc
    26881 cagtacgccg atcccgctgg ggcaaagtca ggatattcag ccgctggtgg aagtggaaaa
    26941 agaggtgatt ctggcggcac tggagaaaac gggcggcaac aaaactgaag ccgcccgtca
    27001 gttagggatc acgcgtaaaa cgttattggc aaaactgtcg cgttagttct gctcgcgttc
    27061 gatagcacgc cagccgatat ctttccggca gaagcagtcg tcccagtgga tatcggtcat
    27121 taaggcatag gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg tgacgcacag
    27181 tacgcgtccg ccgttggtta ccacctgctc gtcatccgcc agttttgtgc ccgcgtggaa
    27241 cactttgccg tcagccactt cttccagcgg caggccgtgg atcacatcgc cggtgcggta
    27301 atcgcccgga tatccacccg cagccatcac cacgccgaga gaagcgcgtt catcccactc
    27361 ggacgttttc tcgtccagct tgccttcaca ggccgccagg cagagctcaa ccagatcgga
    27421 cttcatgcgc agcataatcg gctgggtttc cggatcgcca aagcggcagt taaattcaat
    27481 aaccttcgga ttgccctgtt tgtcgatcat caggcccgca tagagaaaac cggtgtaggt
    27541 gttgccttcc gccgccatgc ctttcacggt tggccagata atacgttcca tggtgcgctg
    27601 atgaacgtca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc cggtgtttgg
    27661 tccggtatct ttatcgccta cgcgtttgtg atcctggctg gtagccatcg gcagcacatg
    27721 ctcgccgtcc accatcacga taaagctcgc ttcttcgcca tcgaggaact cttcgataac
    27781 gatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa cagccgcttc
    27841 cgcttcttcc agcgtcatcg ccacgataac gcctttcccg gcagccagac cgtccgcttt
    27901 aatgacgatt ggcgcgcctt tctcacgcag atacgccagc gcaggttcta cctcggtgaa
    27961 gttctggtat tccgccgtag ggatcttatg gcgggccagg aaatctttgg taaacgcttt
    28021 tgagccttcc agttgggccg cacctgcggt tgggccgaag attttcagcc cggcggcgcg
    28081 gaaggtatcg accacgcctt tcaccagcgg cgcttccggg ccgacgatgg tcagatcaat
    28141 cttttcgttt tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccaa tagcaacgtt
    28201 ttgcagcgcg ggttccagcg cagtgcctgc attacccgga gcaacaaaaa cagtctcaac
    28261 cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt taccaatcac
    28321 taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt gaagagcatc
    28381 gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggatgga accgccaggc
    28441 tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg gaacgggaag
    28501 aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcagc agctttaata
    28561 ccggcgattt tcgcggagta cacgcggctc atctggcccg cgccaatacc gatggtcata
    28621 ttgtttttgg catagacgat agcattggat ttcacgaact tcgccacttt ccagcagaac
    28681 agcgcatcgc gcagttcctg ttcggtcggc tgacgttggg tgacgacgcg cagttcttcc
    28741 gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac gcgtttgaaa
    28801 tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac gttctgcttg
    28861 gcggcggtga ttttcagggc ttcttcgctg gcggaaggcg caataatcac ttcgacaaac
    28921 tgacgagaaa tgatggcctg tgcggtttcc gcatccagct cgcggttaaa ggcaatgatg
    28981 ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc aagaatggaa
    29041 ttgccgatag ctacaccgca agggttggcg tgcttcacaa tcacacatgc cggctcggcg
    29101 aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata agagagggct
    29161 ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc ttctatatag
    29221 aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt aatgaagttc
    29281 aggttcagcg tgcgtgggaa gcgaccagcg gcttctttgc tttcaccgtg gtacgccgga
    29341 accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg ttcgaaggct
    29401 ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc atccatctct
    29461 ttaataatgg cgtcgtagtc gctgctcttc accacgattg cgacatcttt atggttctta
    29521 gcggcggagc gcaccatggt cgggccgccg atatcgatgt tctcaaccgc atcttccagc
    29581 gagcaacctt cacgggccac ggtctgggcg aacggataca ggttaacaac caccatatcg
    29641 ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg gcccagaatg
    29701 ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc cgggaaaccg
    29761 gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg ggcagtgccc
    29821 cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc gacgataccg
    29881 gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg catggtaaat
    29941 cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa actcagctaa
    30001 cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt ttgcgcaacg
    30061 ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggttac cgtgggaaaa
    30121 atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta taaagcgggg
    30181 ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg cggcctgcgg
    30241 ag
//
LOCUS       DAZGCS010000058        30218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGCS010000058 DAZGCS010000000
VERSION     DAZGCS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.058"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK01_002728"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK01_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO8097658.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK01_002729"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK01_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8097659.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK01_002730"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK01_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO8097660.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHK01_002731"
     CDS             complement(1087..1581)
                     /locus_tag="ACHK01_002731"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO8097661.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK01_002732"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK01_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO8097662.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK01_002733"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK01_002733"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO8097663.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK01_002734"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK01_002734"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO8097664.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSDTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK01_002735"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK01_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO8097665.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK01_002736"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK01_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO8097666.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK01_002737"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK01_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO8097667.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHK01_002738"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHK01_002738"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO8097668.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHK01_002739"
     CDS             complement(9275..9769)
                     /locus_tag="ACHK01_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO8097669.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHK01_002740"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHK01_002740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8097670.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHK01_002741"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHK01_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO8097671.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHK01_002742"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHK01_002742"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8097672.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHK01_002743"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHK01_002743"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO8097673.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHK01_002744"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHK01_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO8097674.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHK01_002745"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHK01_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO8097675.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQSSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHK01_002746"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHK01_002746"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO8097676.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAETRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLNVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHK01_002747"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHK01_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO8097677.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17951..18877
                     /locus_tag="ACHK01_002748"
     CDS             17951..18877
                     /locus_tag="ACHK01_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO8097678.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGEIGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18928..19185
                     /gene="yqhH"
                     /locus_tag="ACHK01_002749"
     CDS             18928..19185
                     /gene="yqhH"
                     /locus_tag="ACHK01_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO8097679.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19228..21447)
                     /locus_tag="ACHK01_002750"
     CDS             complement(19228..21447)
                     /locus_tag="ACHK01_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO8097680.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21558..22970)
                     /gene="ftsP"
                     /locus_tag="ACHK01_002751"
     CDS             complement(21558..22970)
                     /gene="ftsP"
                     /locus_tag="ACHK01_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO8097681.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23045..23782)
                     /gene="plsC"
                     /locus_tag="ACHK01_002752"
                     /gene_synonym="parF"
     CDS             complement(23045..23782)
                     /gene="plsC"
                     /locus_tag="ACHK01_002752"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO8097682.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24015..26273)
                     /gene="parC"
                     /locus_tag="ACHK01_002753"
     CDS             complement(24015..26273)
                     /gene="parC"
                     /locus_tag="ACHK01_002753"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO8097683.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26819..27301)
                     /locus_tag="ACHK01_002754"
     CDS             complement(26819..27301)
                     /locus_tag="ACHK01_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO8097684.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27354..27746)
                     /gene="ygiW"
                     /locus_tag="ACHK01_002755"
     CDS             complement(27354..27746)
                     /gene="ygiW"
                     /locus_tag="ACHK01_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO8097685.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27898..28557
                     /gene="qseB"
                     /locus_tag="ACHK01_002756"
     CDS             27898..28557
                     /gene="qseB"
                     /locus_tag="ACHK01_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO8097686.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28554..29903
                     /gene="qseC"
                     /locus_tag="ACHK01_002757"
     CDS             28554..29903
                     /gene="qseC"
                     /locus_tag="ACHK01_002757"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO8097687.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29949..>30218)
                     /locus_tag="ACHK01_002758"
     CDS             complement(29949..>30218)
                     /locus_tag="ACHK01_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO8097688.1"
                     /translation="LIDAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLL
                     TPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7450 a         8074 c         7638 g         7056 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggta
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgtcg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gagatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgtgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cgactggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgaaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg aatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tataagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactaca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc acccgctgat gcaacaacgc cagctacatg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaactccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg cattgtcgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc
    17881 agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc aacggagagg
    17941 tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca
    18001 cgcacagccc ccctcactga ccgtagaaca aacagtccgg catatttatc agaactataa
    18061 atcagatgcc actgcccctt attttggtga aatcggagag cgggcgataa cttctgcgcg
    18121 tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct
    18181 ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag aaagcgttgc
    18241 gataacccaa actgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga
    18301 tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat
    18361 tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa
    18421 aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact
    18481 ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg
    18541 ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc ccgatgaaga
    18601 tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct
    18661 attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgtattca
    18721 tgttcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt tacagcggcg
    18781 cgaaggcaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca
    18841 gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata acaattagcc
    18901 ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt
    18961 ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc
    19021 aatgtgcaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcactacgc
    19081 ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgctcag
    19141 gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg
    19201 cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag ggcgtttctt
    19261 cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg tagcaggcgt
    19321 ctgacgctgg gtcgccatcg gcgtatgttt cgtcaacgcc ggacgggtat tgcggttctg
    19381 gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat cgcgacggct
    19441 gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg cggattaacg gccagtttgc
    19501 cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc ccttcggtac
    19561 gaagacgtct tcgctcttat aaccaatctt cgccagcggg tttttcccgg tgtaatacat
    19621 ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga gacggaaacg
    19681 atgctttttc agccacagcg ccagattcac catatcttca tcacgcgtac cggggtgcgc
    19741 ggagatgaaa taggggatca gatactgctc tttacctgcc tgttttgagt aagtatcgaa
    19801 cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg ataacggccc
    19861 ttcttcggta tgttccgggg caatcttcag ataaccgccg acgtgatggg tcgccaactc
    19921 tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg cgatgaggat
    19981 ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg gttcgtggtt
    20041 agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac aagtttgttc
    20101 agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac cgagatcgga
    20161 aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat taatgatcga
    20221 atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac agaaagaaca
    20281 gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt aagccggaat
    20341 acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa aaacgctgtc
    20401 catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc ggtcaccgtg
    20461 tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg tggtgcagaa tgcgcgaagc
    20521 atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca acacgtaggt
    20581 tttttcccac ggtttcgggc gcggtggctg cacggttacg gctttggctt cctgcttttt
    20641 cggtgccacc ggtttgttat ccgcgcacgg caaatcttca ccatacggat gcgggattgg
    20701 gtcgattttt ccaggggtat caagacgggt ggaatccacg ccgctccagc caggcagcgc
    20761 ctctttcacg ataatcgcgg tattacgcac atcgcggatt tcactaatcg gctcgcccat
    20821 cgccagacgg tgcgccacct ccaccagcgg acgctcaccg ttaccaaaca tcagcatgtc
    20881 ggctttcgaa tccaccagca cggaacggcg cacggtatcg gaccagtaat cataatgtgc
    20941 ggtacggcgc agactagcct caataccgcc gaggatcacc ggtacatctt tccacgcctc
    21001 tttacaacgc tgggtataaa ccagtgtggc gcgatccggg cgcttacccg cgacgttatc
    21061 cggcgtgtag gcatcgtcat gacgtaaacg gcgatcggcg gtataacggt tgatcatcga
    21121 atccatgttg ccagcagtaa caccgaaaaa cagattcggt ttacccagac gcatgaaatc
    21181 gtctttgctg ctccagtctg gctgggcgat gatcccgacg cgaaagccct gtgcttccag
    21241 catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt cgccagtaac
    21301 caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag acatcggcaa
    21361 aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca ggtcgcgatc
    21421 cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa aataatcaaa
    21481 ggccggggat tataagccgg aacgaaagag aaatcgaaag gtattccata ctcgccctcc
    21541 tcgggcgagt atgaagatta cggtaccgga ttgaccaaaa gttgccctat cgagccacgg
    21601 tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca ggaaggctga
    21661 ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt ccagcctctg
    21721 tcttccggga acggcatcgc gccattcacg ttacggatct ggaacattac gccttcaata
    21781 tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgcg
    21841 gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc atcacccaga
    21901 ctgatatcgc gactgcgaat tggcgaacca gccatgattt cagttggcag caagcgcatc
    21961 ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag caccagggta
    22021 gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat gctcgccgct
    22081 tcgccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat ctcgcggcgc
    22141 tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa tccctgatcg
    22201 ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg acgagagttc
    22261 gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta cgggctttgt
    22321 acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc gttgtattct
    22381 ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc atccacacca
    22441 taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca cattccggca
    22501 aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata ccacagagta
    22561 gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga catcatccgt
    22621 gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat tgagacattt
    22681 tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac gcggatagtc
    22741 ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg cgtaaatgac
    22801 caatgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc aagtagcggc
    22861 ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac agcgcctgca
    22921 caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat atttgctcca
    22981 tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc
    23041 cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc tcggcgattt
    23101 tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct ttgccatact
    23161 gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc agtcgattaa
    23221 gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg cccgccgcaa
    23281 ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc
    23341 cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg acttccgcaa
    23401 tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg gttaaccagt
    23461 acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc accgtcggcg
    23521 gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta gcgatataga
    23581 tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc aggccgaaca
    23641 gcggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg ttacgcgggc
    23701 tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc acggtaataa
    23761 taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg cattgtacgt
    23821 cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg tattgttaat
    23881 cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg gggttgaata
    23941 cgctgccgga tgacgactta acgtttcatc cggcgttcct tgcaagcggg aggaaacagg
    24001 ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc
    24061 tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca
    24121 gtgactttct gtaactcttc cgggcgcagt ttaattttgc gtttcccaac atgaatggtc
    24181 agcgtgcttt gcggcggcag aacgtacaat tgcgccagac catcttctcc acgcgcggct
    24241 tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg
    24301 cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca
    24361 tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcctta
    24421 cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg
    24481 gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgcccca
    24541 ggcggcaacg ttaatttgcc ggtgagtggt tcgccctgac cacgcgccga cggcagcgta
    24601 atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc
    24661 ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc cggcgcgtcg
    24721 atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt
    24781 tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc
    24841 gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta
    24901 ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagttca
    24961 ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg
    25021 aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt
    25081 tcatcttcgt tacgaatgat ctcaatcact tcgtcgatgt tgagaaacgc caccagcaaa
    25141 ccttccagga tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc
    25201 acggtatcgc gacggaacac cagccattcg gagaggattt ccagcaggtt tttcaccgcc
    25261 ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc cagatcggtg
    25321 gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc
    25381 accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc
    25441 tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc
    25501 ggtaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact
    25561 gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat aatttccgct
    25621 tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc
    25681 tggtcgatta atgcgattgc cgcctgagct acttcacgca ggttatgcgg tggaatatcg
    25741 gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgagca
    25801 ggcagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc
    25861 tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc
    25921 attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc
    25981 ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca ggcgctatcg
    26041 ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg
    26101 gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg aacaggttta
    26161 agaccatcgc caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag
    26221 taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct
    26281 gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat
    26341 ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc
    26401 gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacttga tcggcataga
    26461 gggcagcaca aggttgcgcc catgccacac cactcggcat gggggtccgc tccgggcgat
    26521 tcgagaagtt gaggttatgc gagtccaggg aagcctaaag catcaaagta gtcgactgta
    26581 agtatacggt tcagcccagt ttagctgtgt ctccaccagt gccccgcgcc tcctgccata
    26641 agtgcggctt gctcatggat acccccgaga cggcttacgg cgcaccggat aataaatttc
    26701 gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata cgcctgtggc
    26761 attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag attccccatc
    26821 acgccaacgg cacataaaca tccgtttcca gtcgctgctc tgtcacccct tcggcaagat
    26881 tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca ggcagccagt
    26941 ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca
    27001 cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta tcgggaatcg
    27061 gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg gtggcagggt
    27121 cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc gtctctttac
    27181 gccacatgat aaatttcgcc acactgtagt tgagcaactc agggctacag cggtgcggca
    27241 acatcgccac agggattgat gggaaatcga taatgtttac atccagtgtc aggtttgtca
    27301 ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa caattacgga
    27361 tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt atcgacttca
    27421 ccctgaatct caaccgtatc tttcggcgtc accgtcacac cgttccagcg tttgtggtcg
    27481 atatcaacat tgatagtacc gctggcatct ttaaacacgt agagatcgtc agagatgcgt
    27541 tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt tgcgctttct
    27601 acggtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg cgttgccgat
    27661 gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cgctgcacag ggccattact
    27721 gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg tttccgagca
    27781 tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta gtgctgtaca
    27841 gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga tgaaaaaatg
    27901 cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac gggccttagt
    27961 aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga ggcgctttat
    28021 agcgcgcctt atgatgcggt gatcctggat ttaaccttac caggcatgga tggtcgcgat
    28081 attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg tactgatcct gaccgcgcgc
    28141 gatgcgctgg aggaacgtgt agaagggctg cgtctgggag ctgacgatta tctgtgtaaa
    28201 ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg aaccaacggc
    28261 caggccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa acgtatcgcc
    28321 acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct ggaattactg
    28381 atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact gtatacctgg
    28441 gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc
    28501 ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga gaaatgaaat
    28561 ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg
    28621 tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac gtcgatgaat
    28681 tgttcgacac ccaactgatg ctgtttgcca agcggttaag tacgctcgat ctcaacgaaa
    28741 tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt catgttgatg
    28801 acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt aacgatggcg
    28861 ataacggaga agatattcct tatagctatc aacgggaagg ttttgctgac gggcaactgg
    28921 tgggcgataa agatcaatgg cgttttgtct ggatgacctc acctgatggc aaatatcgca
    28981 tcgttgttgg tcaggaatgg gaataccgtg aagacatggc gctggcgatt gttgccgggc
    29041 aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg gtactattgg
    29101 gtcgtgaact cgcgccgctg aacaaactgg cgctggcgct gcgtatgcgt gaccctgact
    29161 cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcgtccgttg gttgagtcgc
    29221 taaatcaact gttcgcccgc acacatgcga tgatggttcg tgaacgacgc tttacctccg
    29281 acgcagctca cgaactacgt agcccgttaa cggcgctgaa agtgcaaacc gaagttgcgc
    29341 agctttctga cgatgatccg caagcgcgga aaaaagcgct gcttcaatta cattacggga
    29401 tcgatcgcgc tactcgtctg gttgatcaac tgctcactct atcgcggctg gactcactgg
    29461 ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctccaatcg tcggtgatgg
    29521 atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc aatgcccacg
    29581 gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat ttgctggata
    29641 acgccgtgcg ctacagtcca cagggcagcg tgatagacgt cacgctgaat gctgataatt
    29701 tcatcgtgag ggataacggc cccggtgtga cgccagaggc tctggcgcga attggcgaac
    29761 gcttctatcg cccccccgga caaaccgcta ccggcagtgg acttgggcta tcgattgtcc
    29821 agcgaatcgc caaattgcat ggcatgaatg ttgagtttgg gaatgcggaa caaggtggat
    29881 ttgaggcgaa ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat
    29941 agattttact aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga
    30001 caaataccga gcgagaatac atatacccaa aactcgaaca tttcccgcat aaagagtttt
    30061 cttaagataa gaataataag tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat
    30121 atacgatctt cgcctgaaac ggaaggtata ctgcaaatat tgtctcccga ctcaagcatc
    30181 cagtcatact tattgaaagc aaaaaaagca tctattaa
//
LOCUS       DAZGCS010000059        30144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGCS010000059 DAZGCS010000000
VERSION     DAZGCS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.059"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..3857
                     /gene="yfaL"
                     /locus_tag="ACHK01_002759"
     CDS             123..3857
                     /gene="yfaL"
                     /locus_tag="ACHK01_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO8097689.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGDGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3985..4707)
                     /gene="ubiG"
                     /locus_tag="ACHK01_002760"
     CDS             complement(3985..4707)
                     /gene="ubiG"
                     /locus_tag="ACHK01_002760"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO8097690.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4854..7481
                     /gene="gyrA"
                     /locus_tag="ACHK01_002761"
     CDS             4854..7481
                     /gene="gyrA"
                     /locus_tag="ACHK01_002761"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO8097691.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7630..9318
                     /gene="yfaA"
                     /locus_tag="ACHK01_002762"
     CDS             7630..9318
                     /gene="yfaA"
                     /locus_tag="ACHK01_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO8097692.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHK01_002763"
     CDS             9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHK01_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO8097693.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9962..14476
                     /locus_tag="ACHK01_002764"
     CDS             9962..14476
                     /locus_tag="ACHK01_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO8097694.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKVQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14477..16126
                     /gene="yfaQ"
                     /locus_tag="ACHK01_002765"
     CDS             14477..16126
                     /gene="yfaQ"
                     /locus_tag="ACHK01_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO8097695.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16131..16907
                     /gene="yfaP"
                     /locus_tag="ACHK01_002766"
     CDS             16131..16907
                     /gene="yfaP"
                     /locus_tag="ACHK01_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO8097696.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17182..20031
                     /gene="rcsC"
                     /locus_tag="ACHK01_002767"
     CDS             17182..20031
                     /gene="rcsC"
                     /locus_tag="ACHK01_002767"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO8097697.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20231..20881)
                     /gene="rcsB"
                     /locus_tag="ACHK01_002768"
     CDS             complement(20231..20881)
                     /gene="rcsB"
                     /locus_tag="ACHK01_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO8097698.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20898..23570)
                     /gene="rcsD"
                     /locus_tag="ACHK01_002769"
     CDS             complement(20898..23570)
                     /gene="rcsD"
                     /locus_tag="ACHK01_002769"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO8097699.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24308..25411
                     /gene="ompC"
                     /locus_tag="ACHK01_002770"
     CDS             24308..25411
                     /gene="ompC"
                     /locus_tag="ACHK01_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8097700.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25523..26578
                     /gene="apbE"
                     /locus_tag="ACHK01_002771"
     CDS             25523..26578
                     /gene="apbE"
                     /locus_tag="ACHK01_002771"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO8097701.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSTLMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            26652..27716
                     /gene="ada"
                     /locus_tag="ACHK01_002772"
     CDS             26652..27716
                     /gene="ada"
                     /locus_tag="ACHK01_002772"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO8097702.1"
                     /translation="MKKATCLTDDQCWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVVMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRSLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27716..28366
                     /gene="alkB"
                     /locus_tag="ACHK01_002773"
     CDS             27716..28366
                     /gene="alkB"
                     /locus_tag="ACHK01_002773"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO8097703.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28442..30085
                     /gene="yojI"
                     /locus_tag="ACHK01_002774"
     CDS             28442..30085
                     /gene="yojI"
                     /locus_tag="ACHK01_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO8097704.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKFELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         7321 a         7373 c         8203 g         7247 t
ORIGIN      
        1 attcatcaaa taataacaaa atagccattg catcgggttg gacaatttac aaaagaaaga
       61 taacccatat tgtataatgc gtttaaatat gccgtcttat ataaaatgat ggttagatga
      121 atatgcggat tatctttcta cgcaaggagt atttatctct actcccgtca atgattgcat
      181 ctcttttctc tgctaacggt ttcgcggcgg ccattgattt atgccaggga tatgatatca
      241 aagcgagttg tcacgccagc aggcaaagcc tttcaggcat tacgcaggac tggagtgtcg
      301 ccgatgggca atggctggtt ttttcggata tgaccaataa cgccagcgga ggagccgtat
      361 ttttgcaaca aggagcggaa ttttcactat taccagaaaa tgaaactgga atgactctgt
      421 ttgccaataa caccgttaca ggagaatata ataacggcgg ggccatattt gctaaagaaa
      481 actcaacgct gaatcttact gatgttattt tttctggtaa cgtcgcaggc ggcgatggtg
      541 gcgcaatcta ttcttctggt actaatgata ctggtgccgt cgatttacgt gtcactaacg
      601 ccatgtttcg caataatatc gctaatgatg gcaaaggtgg cgcaatttat accattaata
      661 atgacgtcta tttaagtgat gttatttttg ataacaacca ggcatataca tcaacaagtt
      721 acagtgatgg cgatggcggg gcaatcgatg ttaccgataa taatagcgac agcaagcatc
      781 cttcaggtta tacgataata aataacactg cctttacaaa taacactgcc gaaggttatg
      841 gcggggcgat atataccaat agcgcgacgg ctccctatct tattgatatt tctgttgatg
      901 acagctacag ccagaacggc ggcgtgttag tggatgagaa caacagcgca gcaggctatg
      961 gagatggtcc ttccactgcg gcgggtggct ttatgtatct cggcttaagt gaagttacct
     1021 ttgatattgc cgacggaaaa acgctggtta ttggcaatac agagaatgac ggagctgttg
     1081 actctattgc tggtaccggg ttgatcacca aaacaggttc cggcgatctg gtacttaatg
     1141 cagataacaa tgactttact ggtgagatgc agattgaaaa cggtgaagtt accctgggcc
     1201 gcagcaactc cctgatgaat gtcggcgata cgcattgcca ggacgatccg caagactgct
     1261 acggtctgac gatagggagt attgataagt accagaatca ggcagagcta aacgttggct
     1321 ccacccaaca aacctttgtg cactcattga cgggctttca gaatggcact ttaaatatcg
     1381 atgctggtgg caatgttact gttaatcaag gcagttttgc tggcaccatc gaaggtgctg
     1441 gtcagctcac cattgcgcaa aacggcagct acgtgctgtc gggggcgcag tcgatggcgc
     1501 taaccggcga tatagtcgtt gatgatggtg cggtgctttc gctggaaggc gacgcggcag
     1561 atctcgccgc tctccaggac gatccgcagt cgatcgtgtt aaacggcggt gtgctcgatc
     1621 tctctgattt ctccacctgg cagagcggca catcatacaa tgatggcctt gaagtcagtg
     1681 gcagcagcgg aacggttatc ggcagtcagg atgtggtaga tcttgcaggt ggcgacaatt
     1741 tgcatatcgg cggcgacggg aaagatggcg tctacgtggt ggtcgatgcg agcgacgggc
     1801 aggtaagtct ggcaaacaat aatagttatt tgggcacaac acaaatcgcc tccggtacgc
     1861 tgatggtgag cgacaactcg cagcttggag atacccacta taaccgccag gttatcttta
     1921 ccgataagca acaagaaagc gtgatggaga ttactgccaa tgtcgatact cgctctacga
     1981 cgactgagca tgggcgtgat attgaaatgc gcgccgacgg tgaagtggca gttgatgcgg
     2041 gggtagacac gcagtggggc gcactgatgg ctgacagcag cgggcagcat caggatgagg
     2101 gtagcacatt tactaaaacg ggggcgggta cgctggagct gaccgccagc ggtacaacgc
     2161 agtcggcagt acgtgtcgaa gaaggcaccc tgaaaggcga tgttgcggat atctttcctt
     2221 atgcttcgtc actgtgggtc ggtgatgggg cgacgttcgt tactggcgcg gatcaggata
     2281 ttcagtctat cgatgctact tccagcggca ctatcgatat cagcgatggt acggttttgc
     2341 gtctgaccgg gcaggatact tccgtcgccc ttaatgcctc attgtttaac ggcgatggga
     2401 cgctggtgaa tgctaccgat ggcgtgacgt tgacaggtga gcttaatacc aaccttgaaa
     2461 ctgacagcct gacctatctt gccgacgtga cggttaatgg tgatctgacc aatacgtccg
     2521 gtgcggttag cctgaaaaat ggcgtcgctg gcgatacgct gacggtaaac ggtgattata
     2581 ccggcggcgg tacgctactg ttcgacagcg aattaaacgg cgatgactcg gtaagcgatc
     2641 aattggtgat gaacggtaat actgctggca acacaactgt ggtggttaac tccattacag
     2701 ggattggtga gccgacatcg acaggcatta aagtggttga tttcgcagct gatcccacgc
     2761 agtttcaaaa caatgcacag ttcagtctgg ctggcagtgg ctacgtcaac atgggagcgt
     2821 atgactacac gctagtggaa gataacaacg actggtatct gcgatcgcaa gaagtaacgc
     2881 cgccatcgcc acctgatcca gacccggacc caacacccga tcctgaccca acacccgatc
     2941 caatccccgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc aataatctgc
     3001 gggcggcaaa tcaggcgttt atgatggagc gacgcgatca tgcaggtggc aatggtcaga
     3061 cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg caactggctc
     3121 agcatgaaga cacttctacg gtgcagctta gcggtgacct gtttagcggg cgctggggcg
     3181 atgatggcga gtggatgctt ggggcggttg gtggctacag tgataatcag ggcgagagcc
     3241 gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc gttgggctga
     3301 cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat agctggctgc
     3361 aatacgcgtg gtttaacaat gatgtttccg aacaagacga tggcacagat cattaccact
     3421 cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg cgtggtgtgg
     3481 tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat tttaccgccg
     3541 ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg ctgggtttac
     3601 acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat tattatcacg
     3661 atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat gcggtgaagc
     3721 gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg tccctgcgcg
     3781 gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg tttttgtcga
     3841 tgacggtgaa atggtaatca ctgtctagtc ggcacggttg ccggatgcgg cgtaaacgcc
     3901 ttatccggcc tacggggagc atttgtaggc ctgataagac gcggcaagcg tcgcatcagg
     3961 cattggctcg gccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc
     4021 cacgccgggg ccgagtttaa aagtattagt gatcgggttg taatgtagcc cggttatatg
     4081 tcgctcttta aggcttgtct gatctaccca gcccagcaat tctgccggtt taataaactt
     4141 cttcacatca tgcgtgcctt tgggcaccat gcgcaaaata tattccgcac caaccaccgc
     4201 catcagccat gacttgccgt tgcggttaag tgtcgagaaa aagacatcgc cgcccggttt
     4261 caccagttgc gcacaggctc tgaccactga ctgcggatcg gggacgtgct ccagcatctc
     4321 catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt ccacggtttc
     4381 ctgcacgtaa tccacctgaa tgccgctttc cagtgcgtgc agttttgcca cctgcaatgg
     4441 ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac tctcggccag
     4501 aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc cagcacgctc
     4561 ggcaatatag cccagacgca gcgggttaat gcggtgcagc ggtttgaact caccttccag
     4621 atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg
     4681 gtttaccggc gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac
     4741 gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac tgcgcggctg
     4801 tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg
     4861 accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg
     4921 attatgcgat gtcggtcatt gttggccgtg cgctgccaga tgtccgagat ggcctgaagc
     4981 cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct
     5041 ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact
     5101 cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg
     5161 tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata
     5221 cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg
     5281 tcgatttcgt tgataactat gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa
     5341 ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc
     5401 cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca
     5461 tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca
     5521 ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtgtatatcc
     5581 gcgctcgcgc agaagtggaa gttgacgcca aaaccggtcg tgaaaccatt atcgtccacg
     5641 aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag
     5701 aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca
     5761 tcgtgattga agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac ctctactccc
     5821 agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat ggtcagccga
     5881 agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga
     5941 cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat
     6001 tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc
     6061 ctgcagaagc gaaaactgcg ctggttgcta atccgtggca gctgggcaac gttgccgcga
     6121 tgctcgaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg
     6181 tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg gatctgcgtt
     6241 tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg
     6301 atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtttgatg gaagtgatcc
     6361 gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg
     6421 ccaacagcgc agacatcaac ctggaagatc tgatcactca ggaagatgtg gtagtgacgc
     6481 tctctcacca gggctacgtt aagtatcagc cgctttctga atacgaagcg cagcgtcgtg
     6541 gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg
     6601 tggcgaacac tcacgaccat attctgtgct tctccagccg tggtcgcgtc tattcgatga
     6661 aagtttatca gttgccggaa gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc
     6721 tgccgctgga gcaggacgaa cgtatcactg cgatcctgcc agtgaccgag tttgaagaag
     6781 gcgtgaaagt cttcatggcg accgctaacg gtaccgtgaa gaaaactgtc ctcaccgagt
     6841 tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgacggc gatgagctga
     6901 tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgct gaaggtaaag
     6961 tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg
     7021 gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg
     7081 caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg gaatacccaa
     7141 ccaagtcgcg tgcgacgaaa ggggttatct ccattaaggt tactgaacgt aatggtttag
     7201 tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc gatgccggta
     7261 cgctggtacg tactcgcgtt tcggaaatca gcatcgtagg ccgtaacacc cagggcgtga
     7321 tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt gctgaaccgg
     7381 ttgacgagga agatctggat accatcgatg gcagtgccgc ggaaggggac gatgaaatcg
     7441 ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc ttcatgccaa
     7501 aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt
     7561 cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct atacacaagg
     7621 aatgtggcaa tgagtggtga aaaaaaggcg aaaggctggc ggttctatgg tcttgtaggt
     7681 tttggcgcaa tagcactgct ttccgctggc gtctgggcgt tgcaatatgc tggcagtggg
     7741 ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc tgcaaatcga tctcaatgag
     7801 ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct cctcaccatt
     7861 ccgtttctcc acgatgttct gagcgaagat ttcgttttct attatcagaa tcatgccgat
     7921 cgtctgggca ttgaaggcag cattcgtcgt attgtctatg aacacgatct cacgctgaaa
     7981 gataagctct tttcgtcact cttagatcag cccgcgcagg cagcgctgtg gcacgataaa
     8041 caaggccatc tttcacatta tatggtgctg atccagcgca gtggtttaag caaactgctg
     8101 gagccattgt tgtttgccgc taccagcgac agccagttaa gcaaaacgga aatcagtagc
     8161 atcaagataa atagtgaaac tgttcctgtt tatcagttgc gctataacgg caataacgcc
     8221 ctgatgttcg cgacttatca ggacaagatg ctggtgtttt ccagcacgga tatgttgttt
     8281 aaagatgatc agcaggatac cgaagccacg gcgatcgcag gtgatttgtt gagcggcaaa
     8341 aaacgctggc aagcaagctt tggcctggaa gagcgtgctg ctgaaaaaac gccagtacgc
     8401 cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcggtt aatgccttct
     8461 tttgctggcg tacgcttcga aatgggtaac gacggctggc acagttttgt ggcgttaaat
     8521 gatgaatccg ccagcgtaga taccagtttc gattttacgc cggtctggaa cagtatgcct
     8581 gccggggcca gcttctgtgt ggtggtgccg tattcacacg gtattgccga agagatgctt
     8641 tcgcacatca gtcaggaaaa cgacaagttg aatggggcgt tagacggtgc cgcggggctg
     8701 tgctggtatg cagactcaaa attgcaaacc ccgctgtttg tcggtcagtt tgatggcact
     8761 gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc gcacgaaagc
     8821 aaagcgccag aaggtgtttt gccgataagc cagactcagc agggcgaagc gcaaatctgg
     8881 cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc gcaacccgat
     8941 caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt
     9001 ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa aaaccgcccg
     9061 gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa tccacaaggc
     9121 gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct cgaaccggtt
     9181 ttttataacg ccgcacaaac tttattaatg ccgaagctgg acgctttatc tcaacaaccg
     9241 cgttatgtca tgaagctggc ccagatggaa cccggtgccg cctggcagtg gctacccata
     9301 acctggcagc cgctatgagg cacgggctgc tggcgctgat ttgctggctg tgttgtgttg
     9361 ttgcccatag cgaaatgctg aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac
     9421 gcattgcaca agaacagctc cgccaggggc caagtccacg ctggtatcag caggattgtg
     9481 cgggcctggt gcgatttgcg gcgaacgaga cgctgaaagt tcacgatagt aaatggctaa
     9541 aaagtaacgg tttctctagc cagtatttgc cgccagagat gacgctaaca cctggacagc
     9601 gtcaactggc gcaaaactgg aatcagggga acgggaaaac cggcccctac gtgaccgcga
     9661 ttaatttgat tcagtacaac agccagttta ttggccagga cataaaccag gcgctgcctg
     9721 gcgatatgat ttttttcgat cagggcgatg cccagcactt aatggtctgg atggggcgtt
     9781 acgtcatcta ccacaccgga agcgccacga aaactgacaa cggaatgcgc gcagtcagtc
     9841 tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
     9901 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
     9961 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgcgccgcc
    10021 cgccggggga acattctttt tgcttgctga cagcagtttt agcagcagtg aagaggcgaa
    10081 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
    10141 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
    10201 cctgcatcgc attgtggtgc aaccgcaata tctgggcgac gggctgaaca atacgctgac
    10261 ctggctgtgg gataactggt acggcaaatc tcgccgcgta atgcagcgta ctttctcttc
    10321 tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca atgccattat
    10381 taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat atcccttggt
    10441 ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc aaggggtaaa
    10501 actggaaggc gcatccagca atttcatctc gccgcagccg ggtaacattt atattcctct
    10561 cggccaacaa gagccgggac tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac
    10621 gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggca acgagcttct
    10681 ggtgtggacc gcgggtaaaa aacagggtga agcgaagccc ggctcagaga tcttgtggac
    10741 tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac agcggtacct tgcagttaca
    10801 acatatatcg ccagaacgtt catacattct gggtaaggat gctgaaggcg gtgtttttgt
    10861 ctccgagaac ttcttctacg aaagcgaaat ctacaacacc cgcttgtata tttttaccga
    10921 tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
    10981 cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg tgctggacgc
    11041 taacggcagt ctgttgcaaa ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg
    11101 aagtttccgc ctgccagaaa atgccgttgc cggaggttat gagttacgtc ttgcttaccg
    11161 caatcaggtc tatagcagca gttttcgcgt ggcaaactac atcaagccac atttcgagat
    11221 tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
    11281 actgctctac ccggatggcg agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc
    11341 tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct
    11401 ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc
    11461 cgataaaccg agccgttatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgtgt
    11521 caccaccacc aaagagatcc tcattgaacg cggtctggcg cattactcat taagtactgc
    11581 cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc
    11641 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg
    11701 agagctaccg tcaggcggca aatcctttac cgtcaatttc gctaaacctg gcaactacaa
    11761 tctgacatta cgcgataaag acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg
    11821 taagggcagc acggcgcata ctggtacggt agatatcgtg gcggataaaa cgctgtacca
    11881 gccaggcgaa actgcgaaga tgctgattac ctttccggag ccaattgatg aagcattatt
    11941 gacgctggaa cgcgatcgcg tggaacagca gtcgctgctt tcgcatccgg caaactggct
    12001 gacgttacaa cgtttaaacg atacccagta tgaagcccgg gttccagtga gcaattcctt
    12061 tgcgcctaac atcacttttt cggtgctgta tacccgtaat ggtcagtaca gttttcagaa
    12121 cgccgggatc aaagttgccg ttcctcagct tgatatccgg gtgaaaacgg acaaaaccca
    12181 ttaccagcct ggtgaactgg tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt
    12241 ttctgcgcag ctaacggtag gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat
    12301 cgcgccgaat atcggcaaat ttttctatcc gctggggcgt aacaatgtgc gtaccagctc
    12361 cagtctgtcg tttatcagct acgaccaggc gctctccagc gagccggttg cgcctggcgc
    12421 aactaaccgc agcgagcggc gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga
    12481 taccgcggca tggatgccgt cactcacaac cgataaacaa ggcaaagcgt atttcacgtt
    12541 cctgatgcct gattcgttaa cccgctggcg tatcaccgcg cgtgggatga acggcgacgg
    12601 gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa aatctctaca tgaagtggag
    12661 tatgccaacg gtgtatcgcg tgggcgacaa accggcggca ggactgttta tcttcagtca
    12721 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggcgctgaga tgcgccagac
    12781 gctgacgctg cacaaagggg cgaattatat ttcgctggcg cagaacattc agcaatctgg
    12841 cttgttaagc gcagaactgc aacaaaatgg gcaagtgcag gacagcatta gcacaaaact
    12901 gtcttttgtg gataacagct ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg
    12961 cgagaacgcg ctgatgttgc ccgagcaggc tagcaatatc cggctacaaa gtagcgaaac
    13021 gccgcaggag attttccgca acaatcttga tgcgttagtc gatggaccat ggggtggcgt
    13081 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
    13141 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
    13201 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggtgacgc
    13261 cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
    13321 gcaacaaccg gaatactggc agcatatgct cgacagttat gccgagcagg cggataacat
    13381 gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa
    13441 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaag atgaagattt
    13501 ctctgaggaa gacatccgcg atatcaatga cagcctgatc ctcgatacac cggaatctcc
    13561 actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag tgcagttgaa
    13621 gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagcaatca
    13681 gccgctggcg cacacggttg tgctgctcaa tagcggtggt gacgctaccc agacggccgc
    13741 tattttaagt ggtttgaccg ctgagcaatc cactattgag cgcgcgctgg ccatgaactg
    13801 gctggcgaaa tatatggcga caatgcctcc agttgttttg cctgcgcctg cgggcgcatg
    13861 ggctaaacat aagttaactg gagggggcga agactggcgt tgggttggtc agggtgtgcc
    13921 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
    13981 accggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
    14041 gcttatcccc ggtgaagaag agatgagctt tactctgcag ccggtgacca gcaatgagat
    14101 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
    14161 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
    14221 gggcatttca gtcaataaac ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc
    14281 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga cgggaacggt
    14341 cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg
    14401 ttatgtgcgt tcctatgcac ctgcgcagca aagtgttgcg gcagggagtg aatggaccgg
    14461 gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactct
    14521 gccaacgctt gcggaggaga cgccgttaca actggtgctg cgcggtgcgc agcacgatca
    14581 actgtatcag ctgtcgtcat caggcgtgac caaagtctca gcattacctg actcgctcac
    14641 cacgccgtta gggagtttgt ggaaactgta tgtttacgcc tggctggagg atacccatca
    14701 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
    14761 tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct attttgcgcc
    14821 gcagcggttg catattggcg cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc
    14881 tgcgtggtta gcgtctctta caacgctgaa gccagaaacc tcggtaacgg ttaaatcgct
    14941 gctcgattca ttagcgacgt tacctgcgca aaacaaagct caggaagtct tactggatgt
    15001 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
    15061 aacctggagc tggtttgccg atgataaaca ggaaatccgc cagggtggtt ttgctggctg
    15121 gttaaccgat ggtacaccgc tgtgggttac tggcagtggc accagcaaaa cggtactaac
    15181 ccgttatgcg acggtgctta atcgggtttt gccagtgccg acgcaggttg ccagtgggca
    15241 atgtgtagag gtggagctgt ttgcccgcta tccgctgaag aaaatcacag cggaaaagag
    15301 tacgacggca gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacgt ttactaatgg
    15361 taaccacatt acgtttgtca gccacggtga gacgacctta ctgagcgaaa agggcaaact
    15421 gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
    15481 gaaaagtacg ccccctgagg cagcgaaagc catgaccgtg gcgattcgca cttttttaca
    15541 gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg ccacgcagcg
    15601 tgtttctgct tcgccagcga ccacgggggc gcgaacgatg accgcctgga cgcaggatct
    15661 catctatgca ggcgatccgg ttcattatca tggcagtcgc gctaccgaag ggacactttc
    15721 ctggcggcaa gccacggccc aggcggggca gggggagcgt tacgaccaga tactcgcgtt
    15781 tgcttatccc gacaacagcc ttagccgctg gggagctcca cgctcaacct gccagttatt
    15841 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
    15901 cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
    15961 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
    16021 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
    16081 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
    16141 tttttcttcc gttgttactg gtggcacttt cgcctgtcgc tcacagtgaa ggtgtgcagg
    16201 aagtcgagat tgacgcaccg ctctctggct ggcatccggc agagggcgaa gatgccagtt
    16261 tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
    16321 cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggcc
    16381 ggctggtggt caacggtgcc agtatgccgc agagagttga atccgatggt tcatttgcac
    16441 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa
    16501 gccgacaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt gcacgtttac
    16561 ggctggttct ctcgtgggat acggacaata ccgatctcga tctccatgtt gtcacgcccg
    16621 atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg
    16681 atgtcacgac ggggtacgga cccgagattt tcgccatgcc agcaccagtt cacgggcgct
    16741 atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattgacg accgcccaac
    16801 tgacgctgat caccgatgaa ggatcggtca atgagaaaca ggaaacattt attgtaccga
    16861 tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatcc atcccttcac
    16921 acaacctggc tctctctttt ttcagataag actgctgggc gatctgccga cgccaggata
    16981 taaccattaa caagcgtcgg cagattggtc atttcttatt tttcgtactg agaactgtcc
    17041 atctactccg gctttcctgg ttaaggcaat ggtttctaag cgatacgcat tattgcaatg
    17101 gtgacagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
    17161 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc
    17221 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
    17281 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
    17341 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
    17401 aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
    17461 gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg ccgactccga ttgttccgca
    17521 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
    17581 gataattttt ctgcggctta cgatctcaac cgggtatttt taatcggcag tgataacctc
    17641 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagct
    17701 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
    17761 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
    17821 tatctggcga accggttgca ggcgcttttg ggtgtcgagc agaccatccg gatggagaac
    17881 tttttcttac cgggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
    17941 ctgatttcgc ttaccggacc agaaagtaaa attaagggcg atcctcgctg gatgcaggaa
    18001 cgctcctggt ttggctatac ggaagggttc cgggagctgg tgctgaagaa aaatctgcca
    18061 ccctcatcgc taagcatcgt gtattcggtg ccggttgata aggtgctgga acgcattcgc
    18121 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
    18181 gcgcggatgt acgagcgacg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
    18241 gaacatgagc agttcaatcg caagattgtc gcctccgcgc cagtgggtat ctgtattttg
    18301 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
    18361 cttacgcatg aggaccgcca acgactgacg caaattatct gtgggcagca ggtcaatttt
    18421 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
    18481 cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa
    18541 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
    18601 cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
    18661 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcggc tggtgacggc aatgaacaac
    18721 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa gattgaatcg
    18781 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
    18841 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
    18901 gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
    18961 ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt ccgcgctgat
    19021 ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc gaaagaagtg
    19081 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa cttccagggg
    19141 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg
    19201 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
    19261 cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta aacgctgctg gctggcggtc
    19321 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgtt
    19381 acaacatacg aagggcagga accgactccc gaagatgtgt taattactga cgaggtagtg
    19441 agtaaaaaat ggcagggtag agcggtagtg accttctgtc gtcgccatat tggtattccg
    19501 ctggagaaag cgccagggga gtgggtacac agtgtggctg ccccgcatga gctaccggca
    19561 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
    19621 tcgacggaca aagcggtcag cgataatgac gatatgatga ttctggtcgt ggatgatcat
    19681 ccgattaacc gacgtctgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
    19741 gcgaatgatg gtgtcgatgc gcttaatgta cttagcaaga atcatattga tatcgtgctt
    19801 agcgacgtca acatgccaaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    19861 ggactgacgt tgccggtaat cggagtaact gctaatgcgt tggctgaaga gaagcagcgg
    19921 tgtctggagt ccggtatgga cagctgcctg tcgaagccgg taacgctgga tgtgataaaa
    19981 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggacggataa
    20041 ggtgtttatg ccgcatctgg cattcagtgc aaatgccaga tgcgacgctg acgcgtctta
    20101 tctggcctac tttaatgtaa tctgttgaaa taatgggaat cgtaggccgg ataaggcgtt
    20161 tacgctgcat ccggcaatcg gtgcaaatgc cagataagac actaacgcgt cttatctggc
    20221 ctacagatga ttagtcttta tctgccggac ttaaggtcac agaagagaga taattcagca
    20281 gggcgatatc gttctcgaca cccagcttca tcatcgcaga tttcttctgg ctactgatgg
    20341 ttttaatact gcggttcagc tttttagcga tctcggtcac caggaagcct tccgcaaaca
    20401 ggcgcagaac ttcactctct tttggcgaga gacgcttgtc accgtaacca ccagcactga
    20461 ttttttccaa caggcgagaa acgctttccg gggtaaattt cttccctttc tgcagcgcgg
    20521 cgagagcttt cggcagatcg gtcggtgcac cttgtttcag cacgatccct tcgatatcca
    20581 gatccaatac cgcactaaga atcgccgggt tgttgttcat ggtcagaaca atgatcgaca
    20641 ggcttgggaa atggcgcttg atgtacttga ttaaggtaat gccatcgccg tacttatcgc
    20701 caggcatgga gagatcggta atcaacacat gcgcatccag tttcggcagg ttgttgatca
    20761 gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt
    20821 tgcgaatacc gaacaagact atcggatggt catcggcaat aattacgttc atattgttca
    20881 tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc gctgatgtat
    20941 ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc acataactgc
    21001 ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc cgtttgggct
    21061 aacgcagcaa agtcactggt tgctgcttca gtatacagcc tcttaacatc atccggtact
    21121 gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc atctccgccc
    21181 agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg taagaccgct
    21241 tcctgcatag cgttgctcat attgaagttg acgcacaatt gaccaggccc aatttcccgt
    21301 acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga cggattatcc
    21361 gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat acaggttgca
    21421 ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt aacatccacc
    21481 attacgcaga catcatccag taaacgctct tcttcctctt caacttccgg gtcagctgcg
    21541 agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt gatgtttaaa
    21601 tgaccgccca gtttacgtgc cagttgatcg ctcagccaga atgccagcgg gtccgccttg
    21661 ccatagcgat cgttttgggt ctggttgata aacgggaagt gcaaattatc catttcatga
    21721 atacttacgc cttctcccgt gtccagaatg cggaacgtca ggcggtcttc ggaggactca
    21781 tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc atattgcatc
    21841 agtagcagca aaatacgtcg taaggcatcg cgatcgccgc ggcgcatatc gtgtgctttc
    21901 agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa cactgaaggc
    21961 acaacttcat caattaaatc ctgcacggag aacagcaccg tctcactttt ccagctatca
    22021 tccgcaagca tgttcgctaa ctgtatttca tcaaccagcc gcaccagcac atctgcctga
    22081 ttcgccagtt gtttgctttc cggggcgttg agtttagccg cgctctccgc cagggactgt
    22141 gcgggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc ctgctggttt
    22201 ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc gcgatcctga
    22261 tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc tgcggaacat gcggatctca
    22321 tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc catggtggtg
    22381 atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact tatgacagtg
    22441 cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac tatctcttca
    22501 ttaattgccc gtaaaatgcg caattcgtta ttgaccgccg tgctgggtag actttctgta
    22561 ctgcggctgg agaaatggcg gaatgtggta tagccaaata acgccagcgc cagcaaaccg
    22621 atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcaataa ggtgccataa
    22681 ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggagat ttcaatcttc
    22741 gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattatc attgtctccc
    22801 gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa
    22861 tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg gttgaacgtg
    22921 gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt agaaaaactt
    22981 tcgcgttcat cgagggcgtt ggcctgttgc aacatttctg cacgacgtga atcaacaatg
    23041 tcactgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag ggttgagatc
    23101 agcaccagac tgttatcctg accattcagg taatacatcg accacggtac attttctgcg
    23161 ccccacaatg tgtccagata ggtggacatc cgttgagtca tctcaagcgt tgagttgtcg
    23221 tgagagccaa agattaacgc ttccgttttg cggcgcggtt tttccagata gtagacatcc
    23281 tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt cgcggcaatg
    23341 ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg gatagcgtgg
    23401 gtaatgtcga caatctggta acttttatct ttcagccagg cgttaacggc gctttgtacc
    23461 attaccccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg ggtaatgctc
    23521 cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat
    23581 gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt gaaattccag
    23641 actcttacct gattgatgat tctctattct cccgtgatat ttatcatcct gcattggggg
    23701 aaatagatgt acaagcgcca ttttgctgta caaataacct acaaaaagcc caacaaaaat
    23761 aattgcaaat aaacaaagat tgctggaaat tatgctgatg ttaattattt gtgaaatagt
    23821 taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg taaagaaggg
    23881 taaaaaaaac ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga
    23941 atgacggtaa taaataaagt taatgatgat agcgggagtt attctagttg cgagtgaagg
    24001 ttttgttttg acattcagtg ctgtcaaata cttaagaata agttattgat tttaaccttg
    24061 aattattatt gcttgatgtt acgtgcttat ttcgccattc cgcaataatc ttaaaaagtt
    24121 cccttgcatt tacattttga aacatctata gcgataaatg aaacatctta aaagttttag
    24181 tatcatattc gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta
    24241 atgagggtta atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt
    24301 taataacatg aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc
    24361 agcaaacgct gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt
    24421 agacggcctg cactatttct ctgacaacaa agatgtagat ggcgaccaga cctacatgcg
    24481 tcttggcttc aaaggtgaaa ctcaggttac tgaccagctg accggttacg gccagtggga
    24541 atatcagatt cagggcaaca gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt
    24601 cgcaggtctg aaattccagg atgtaggttc tttcgactac ggtcgtaact acggcgttgt
    24661 ttacgacgta acttcctgga ccgacgtact gccagaattc ggtggtgaca cctacggttc
    24721 tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt
    24781 cggtctggtt gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag
    24841 cggcgaaggc atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg
    24901 cggatctatc acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa
    24961 acgtactgat gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta
    25021 caccggtggt ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac
    25081 ctataacgca actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc
    25141 tgttgctcag taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa
    25201 aggtaaaaac ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata
    25261 tgttgatgtt ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa
    25321 aatcaacctg ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat
    25381 cgtagctctg ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc
    25441 gggccctttt ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc
    25501 gttcgcatga ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc
    25561 cgcgctcttc tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga
    25621 agttaccgtt cttgaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat
    25681 tgatgccaaa cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga
    25741 tcagctgctt tcgacctata aaaaagattc cacgctgatg cgctttaacg actcgcaaag
    25801 tttgtcgccg tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat
    25861 tggcgcaagg accgatggtg cgatggatat aaccgtcggg ccgctggtga atctgtgggg
    25921 ctttggcccg gaacaacagc cggttcaaat tccgagccag gaacagatcg atgcgatgaa
    25981 agccaaaacc ggcttacagc acctgacggt catcaatcag tcgcatcagc aatatctgca
    26041 aaaagacctg ccggatttat atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga
    26101 tcacctggca cgcttgatgg agcaggaagg tatttcacgt tatctggtgt cagttggtgg
    26161 tgctctggat agccgtggta tgaacggtga aggccagccg tggcgggtag cgattcaaaa
    26221 accaaccgat aaagaaaacg cggttcaggc cgtggtggat atcaacggtc atggcattag
    26281 cacctctggc agctaccgta actattacga actggacggc aaacgtcttt ctcatgttat
    26341 cgatccgcaa accgggcgtc ctatcgaaca caatctggta tccgtgacgg tgattgcccc
    26401 gacggcgctg gaagccgatg cctgggatac tggcttaatg gtactcgggc cggagaaagc
    26461 caaagaggtt gttcgccggg aagggctggc ggtctatatg atcaccaaag aaggtgatag
    26521 ctttaaaacc tggatgtcgc cgcagtttaa aagcttcctt atcagcgaaa aaaattaaag
    26581 cgcaagattg ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca
    26641 gggagctgat tatgaaaaaa gccacatgct taactgacga tcaatgctgg caatctgtct
    26701 tagcccgcga cccgaatgcc gacggcgaat tcgttttcgc cgtgcgtacc acaggcatct
    26761 tttgccgtcc gtcttgccgc gccagacatg ctttgcggga aaacgtctcc ttctacgcaa
    26821 atgccagcga ggcactcgcc gctggctttc gcccctgcaa acgttgtcag ccagaaaaag
    26881 ccaatgccca gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg
    26941 aaacgcctgt aacgctggaa gccttagccg accaggtggt gatgagtcca tttcatctac
    27001 atcggttgtt taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg
    27061 ctcgccgttt gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg
    27121 ccggattccc cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg
    27181 ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg
    27241 agctgggtcg tagcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg
    27301 atgatgacgc gacactaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc
    27361 ctgccgatct gatgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg
    27421 atacgccgct gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc
    27481 aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg gctaacgcca
    27541 tcggcaaacc gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta
    27601 tcataatacc ctgtcatcgg gtggtccgtg gtgatggcac actttccggt taccgctggg
    27661 gcgtgtcgcg taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt
    27721 ggatctgttt gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt
    27781 acggcgtttt gcttttaatg ctgcggagca actaatccgc gatattaatg acgttgccag
    27841 ccagtcgccg tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac
    27901 caactgtggg catctgggct ggacgagcca tcggcaaggt tatctctatt cgcccattga
    27961 tccgcaaaca aataaaccgt ggcccgccat gccacagagt tttcataatt tatgtcaacg
    28021 tgcggctacg gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta
    28081 cgctcctggc gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc
    28141 aattgtttct gtttctctgg gcttacctgc gatttttcaa tttggtggcc tgaaacgtaa
    28201 tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtgtggg gcggtgagtc
    28261 gcggctgttt tatcacggta ttcaaccgtt gaaagcgggg tttcatccac tcaccaccga
    28321 ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt
    28381 attgctgtgc gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg
    28441 catggaactt cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc
    28501 gctaagcctc gccagtgcgg cattaggcat tggcttaatt gcttttatca atcagcgcct
    28561 tatcgaaacg gcggatacca gtctgctggt attgccggag tttctgggat tattgctgct
    28621 gttgatggca gtcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt
    28681 ttaccgactg cgtagtgaat ttatcaagcg gattctggat accaacgtcg agcgcattga
    28741 acaactcggt agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat
    28801 tgcttttgtg cgtcttccgg aactggtgca ggggatcatt ctcactatcg gttccgcggc
    28861 gtatctgtgg atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcgatcac
    28921 catctggggt ggttttgtac tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga
    28981 aaccgaagac aggctgtata cggattttca aactgtactc gaagggcgca aagagctgac
    29041 tctgaaccgg gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga
    29101 gtatcgccac catattatcc gcgcagacac cttccatctt agcgccgtga actggtcaaa
    29161 catcatgatg ttgggcgcaa tcggcctggt gttctggatg gcgaacagtc tcggttgggc
    29221 tgataccaac gttgccgcga cctattcgtt gacgctttta ttcctgcgta cgccgctgct
    29281 ttcggcagtt ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa
    29341 caaattcgcg ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg
    29401 gcaaacgctg gagctgcgta acgtgacgtt tgcttatcag gataacgcgt tttccgttgg
    29461 tccgattaat ctcaccatca aacgtggcga gctgctgttt ctgattggcg gcaacggtag
    29521 cggaaaatcg acgctggcga tgttgttgac gggcttgtat caaccacaaa gcggcgaaat
    29581 cttgctggat ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc
    29641 ggcagtgttt accgatgtct ggctgtttga tcaactgctg gggccggagg gtaaacccgc
    29701 taacccgcaa ctggttgaga agtggctggc gcagctgaaa atggctcata agtttgagtt
    29761 aagcaacggg cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct
    29821 gttgctggcg ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca
    29881 ggatccacac ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg
    29941 taaaactatt ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct
    30001 ggaaatgcgc aacgggcaac ttagcgagct gacgggcgaa gagcgcgatg ctgcttcgcg
    30061 tgatgccgtt gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca
    30121 ggtcaatatg ctaaaaatcc cttc
//
LOCUS       DAZGCS010000060        29848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGCS010000060 DAZGCS010000000
VERSION     DAZGCS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.060"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            137..1528
                     /locus_tag="ACHK01_002775"
     CDS             137..1528
                     /locus_tag="ACHK01_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO8097705.1"
                     /translation="MISFTPRLYQELIINHEIGISRCNIWAGMGMGKTVATLTTLEDL
                     FMAGAETRPALVLAPLRVAASTWPDEAVKWGHLRNIEVQPIIGNAKARSTALANSNAS
                     VFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVRR
                     WINLTGTPAPNGLVDLWGQAWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPFA
                     HSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELNS
                     EGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVAY
                     HWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGRNILV
                     FFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTVQ
                     DILLDAMKKRGIA"
     gene            1525..1725
                     /locus_tag="ACHK01_002776"
     CDS             1525..1725
                     /locus_tag="ACHK01_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8097706.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(1722..3119)
                     /locus_tag="ACHK01_002777"
     CDS             complement(1722..3119)
                     /locus_tag="ACHK01_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8097707.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKSTFGE
                     REVFSDVTYSRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            3334..3594
                     /gene="yfhL"
                     /locus_tag="ACHK01_002778"
     CDS             3334..3594
                     /gene="yfhL"
                     /locus_tag="ACHK01_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO8097708.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(3788..3868)
                     /gene="shoB"
                     /locus_tag="ACHK01_002779"
     CDS             complement(3788..3868)
                     /gene="shoB"
                     /locus_tag="ACHK01_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO8097709.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4288..4668)
                     /gene="acpS"
                     /locus_tag="ACHK01_002780"
     CDS             complement(4288..4668)
                     /gene="acpS"
                     /locus_tag="ACHK01_002780"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO8097710.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(4668..5399)
                     /gene="pdxJ"
                     /locus_tag="ACHK01_002781"
     CDS             complement(4668..5399)
                     /gene="pdxJ"
                     /locus_tag="ACHK01_002781"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO8097711.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5411..6139)
                     /gene="recO"
                     /locus_tag="ACHK01_002782"
     CDS             complement(5411..6139)
                     /gene="recO"
                     /locus_tag="ACHK01_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO8097712.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6151..7056)
                     /gene="era"
                     /locus_tag="ACHK01_002783"
     CDS             complement(6151..7056)
                     /gene="era"
                     /locus_tag="ACHK01_002783"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO8097713.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7053..7733)
                     /gene="rnc"
                     /locus_tag="ACHK01_002784"
     CDS             complement(7053..7733)
                     /gene="rnc"
                     /locus_tag="ACHK01_002784"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO8097714.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8006..8980)
                     /gene="lepB"
                     /locus_tag="ACHK01_002785"
     CDS             complement(8006..8980)
                     /gene="lepB"
                     /locus_tag="ACHK01_002785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO8097715.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(8996..10795)
                     /gene="lepA"
                     /locus_tag="ACHK01_002786"
     CDS             complement(8996..10795)
                     /gene="lepA"
                     /locus_tag="ACHK01_002786"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO8097716.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(10993..11472)
                     /gene="rseC"
                     /locus_tag="ACHK01_002787"
     CDS             complement(10993..11472)
                     /gene="rseC"
                     /locus_tag="ACHK01_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO8097717.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11469..12425)
                     /gene="rseB"
                     /locus_tag="ACHK01_002788"
     CDS             complement(11469..12425)
                     /gene="rseB"
                     /locus_tag="ACHK01_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO8097718.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12425..13075)
                     /gene="rseA"
                     /locus_tag="ACHK01_002789"
     CDS             complement(12425..13075)
                     /gene="rseA"
                     /locus_tag="ACHK01_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO8097719.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13108..13683)
                     /gene="rpoE"
                     /locus_tag="ACHK01_002790"
     CDS             complement(13108..13683)
                     /gene="rpoE"
                     /locus_tag="ACHK01_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO8097720.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(13680..13835)
                     /gene="rseD"
                     /locus_tag="ACHK01_002791"
     CDS             complement(13680..13835)
                     /gene="rseD"
                     /locus_tag="ACHK01_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO8097721.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14091..15713
                     /gene="nadB"
                     /locus_tag="ACHK01_002792"
     CDS             14091..15713
                     /gene="nadB"
                     /locus_tag="ACHK01_002792"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO8097722.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(15698..16435)
                     /gene="trmN"
                     /locus_tag="ACHK01_002793"
     CDS             complement(15698..16435)
                     /gene="trmN"
                     /locus_tag="ACHK01_002793"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO8097723.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            16567..17901
                     /gene="srmB"
                     /locus_tag="ACHK01_002794"
     CDS             16567..17901
                     /gene="srmB"
                     /locus_tag="ACHK01_002794"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO8097724.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(18111..18992)
                     /gene="yfiE"
                     /locus_tag="ACHK01_002795"
     CDS             complement(18111..18992)
                     /gene="yfiE"
                     /locus_tag="ACHK01_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8097725.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            19095..19682
                     /gene="eamB"
                     /locus_tag="ACHK01_002796"
     CDS             19095..19682
                     /gene="eamB"
                     /locus_tag="ACHK01_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO8097726.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(19738..20121)
                     /gene="grcA"
                     /locus_tag="ACHK01_002797"
     CDS             complement(19738..20121)
                     /gene="grcA"
                     /locus_tag="ACHK01_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO8097727.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            20426..21115
                     /gene="ung"
                     /locus_tag="ACHK01_002798"
     CDS             20426..21115
                     /gene="ung"
                     /locus_tag="ACHK01_002798"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO8097728.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(21163..22200)
                     /gene="yfiF"
                     /locus_tag="ACHK01_002799"
     CDS             complement(21163..22200)
                     /gene="yfiF"
                     /locus_tag="ACHK01_002799"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8097729.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            22407..22826
                     /gene="trxC"
                     /locus_tag="ACHK01_002800"
     CDS             22407..22826
                     /gene="trxC"
                     /locus_tag="ACHK01_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO8097730.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            22895..23593
                     /gene="tapT"
                     /locus_tag="ACHK01_002801"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             22895..23593
                     /gene="tapT"
                     /locus_tag="ACHK01_002801"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO8097731.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            23625..26285
                     /gene="pat"
                     /locus_tag="ACHK01_002802"
     CDS             23625..26285
                     /gene="pat"
                     /locus_tag="ACHK01_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO8097732.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            26399..27754
                     /gene="pssA"
                     /locus_tag="ACHK01_002803"
     CDS             26399..27754
                     /gene="pssA"
                     /locus_tag="ACHK01_002803"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO8097733.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            27800..28123
                     /gene="yfiM"
                     /locus_tag="ACHK01_002804"
     CDS             27800..28123
                     /gene="yfiM"
                     /locus_tag="ACHK01_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO8097734.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(28120..29418)
                     /gene="kgtP"
                     /locus_tag="ACHK01_002805"
     CDS             complement(28120..29418)
                     /gene="kgtP"
                     /locus_tag="ACHK01_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO8097735.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(29744..>29848)
                     /gene="rrf"
                     /locus_tag="ACHK01_002806"
     rRNA            complement(29744..>29848)
                     /gene="rrf"
                     /locus_tag="ACHK01_002806"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         7292 a         7805 c         7423 g         7328 t
ORIGIN      
        1 taggatgaat aacactataa actttcatac ttataaaacc aggaataacg aaaagcaaaa
       61 atataataat tttacttttt tcccaaatat ccattttaac ccctaatctc agaatgtgta
      121 taggttatct cttgttatga tatcttttac cccccggtta taccaagaac taattattaa
      181 tcacgaaata ggtatttccc gctgcaatat ttgggcagga atgggaatgg gtaaaaccgt
      241 ggcaacgctc actacgctgg aagatctctt tatggcaggg gcagaaacac gccccgcact
      301 ggtcctcgcg ccgctacgcg ttgcagcaag cacctggcct gatgaagctg ttaaatgggg
      361 gcacctgcgt aatattgagg tacagccgat tatcggtaac gccaaagcgc gctctacggc
      421 gctggcgaac agcaacgcga gcgtgttcac catcaactac gataaccttg tctggctggt
      481 tgaggaattg ggagaacgat ggccgttcgg tactgttatt ccagatgaaa gcacccggct
      541 aaaatccttc cggctacgag gtggtggtaa gcgcgctgcg gcactgggca aagtggcgca
      601 taagtatgtc cggcgctgga taaatctcac cggtacgcca gcaccgaacg gcctggtaga
      661 tttgtgggga caagcgtggt ttgtggacca ggggcaacgt ctcgggcgca cttacggtgc
      721 gtttacctca cgctggttca actcgataca gtttccggga cagagctgga ctaaactgga
      781 accttttgct cactcacagg gtgaaataca gcgagcatta gccgatgtga ccctctcgct
      841 ggatgcggcc gactggttcg atatcaaaga ccccatccat aacgtaatcc gcgtggatat
      901 gcccccgaag gcccgtcagc agtatcgtga aatggaaaag gaaatgttcc tcgagctgaa
      961 tagcgaaggc atcgaagcac cgaacgccgc ggcaaagaca ctgaagtgtc ttcaaatcgc
     1021 cagtggcgca gtatacacag acgacaccgg aagttggtca gaactgcatg acaccaaact
     1081 acaggcgctg gacagcatac tgaccgaagc agctggcgca cctgtgctgg ttgcttatca
     1141 ctggaaacac gatcttgaac gcttgcttaa agcattccct cgcggtcgtc acctcgacca
     1201 ggatccacag acactgcgcg actggaattc cggaaagatt cctgttctct ttgcacaccc
     1261 agccagcgcg ggccacggcc tgaacatgca ggacggcaga aatatactgg tatttttctc
     1321 acactggtgg gacctggagc aatatcagca aattattgaa cgtatcggcc ccaccaggca
     1381 gatacaggcc ggacacaatc gtccggtatt tattcaccac attattgctg ccgacactat
     1441 ggacgaaatg gtgatggagc ggcgcaactc aaaacgaaca gtgcaggaca tcctgctcga
     1501 tgccatgaaa aagagaggta tagcatgagc gagaaacccg acgatttact caccccggat
     1561 gaagtatgcc aaaagttagg tattacacag aaaacgctat gtgagtggaa tattaagcat
     1621 cgtcatcggg ctatcctggc accaattcgt ttcagtgcaa aagtagttcg ttatgagcgc
     1681 cgaaatgtcg acgcttttat tcaaaaatgt cgcagccagt attaacctcg ccgccgtagc
     1741 aatgccacct gcgcaagtat gctccgctcg tgagcctcga aagcttcgcg cttcaacgca
     1801 atctcttcct gtaaaatctc atcagaaaag tcgtaatgtt ctgccatcgg gtcatctgac
     1861 ttgctggagt ggtgaaggca aaggaggctg atttcccttc ggtctgagcg ggaatagcct
     1921 ctttccttca tcaaggcaat aacattgctc ttaaggaatt tacggcacat cgtattaaat
     1981 gcaccgtctt tccctttaac agtcccatca tgttttattc cttttacagc cccgtccggg
     2041 ctgtatgttt tcaccagctt atccagtgat cgttttgaaa atggctgcat tggatcacgt
     2101 ggctgcaaaa atacataatc cctgttgcac tcaggaactg aatcacgcca ggctttctgc
     2161 tcgtcgataa tccgccggat ctcaggcgtt atcggcaggc ggaaagcctt ttgtgttttc
     2221 atagcccctc gcatgccgat aaccccttcc ggataaacaa tttcaccagc ctcctcgtga
     2281 acgtagtccc agcgcaggtt atggacatta atcggacgaa caccagtgat gatcatgaag
     2341 cgaacagcat tcttctggtg tacagaggtg caggcagcaa cattgagcca gagtcgggcg
     2401 attgattcaa tatcggtaaa aagccgtgtg ggggtaggtt tctgtacgcg ggaggaaaca
     2461 taatcatctg gcagactggc ggcaacattg cggccgttgc aaagagtagg tgcgcagaac
     2521 ttccagaacc gacggagctc ggcaaacaac tccagggcgt tattgttcga gcgagtggcg
     2581 atccactcgt ccagcacttc caccagccga ctgtatgtta cgtcgctgaa cacctcgcgc
     2641 tcgccgaacg ttgatttaat ccggtcgata cgcaccccgt aggttgtgaa actgtccggg
     2701 ctcagcttct gacgggctac tttggctttg aggtcatccc ggtacatttc cagcgctgca
     2761 tgtacggact ctgcccgcag tccaccgtca gccataccta gtgctttttc gcgcgccagc
     2821 tggatagcga gctccggcca ctcgccgagc tttttacctt tgaggcccat cttttttgga
     2881 aactcggcgt aaaatgtaac cttaccggct ttgctgaaat cgatacggag atagttctct
     2941 ttttcgtatt tggaacggcg agccacgccg gaagcagcga ggatgatttt ggcggcagca
     3001 acacagattt tcatgtgtgc gctggtatag gggggtttac aggcgtccca tttttcagaa
     3061 gcggctaaaa catcgtcatt attggggcta tccggattat gtgttacagt gcgcggcatt
     3121 ctcaatcctt atctgcgtag gcgcagaaaa caagctcaca catacaagtc ttttctacgg
     3181 gacaaaatgc aatgtgttgc ggttttgtgt tgctggactg agtttatcaa ggttaaatac
     3241 actggatcaa catacagtaa gttaatgaca gtcaagcata caaactcgat acaacttact
     3301 gattttaaaa tgatttaacg gtaatccatt gaaatgtctt tactaattac taaacgctgt
     3361 attaattgtg atatgtgtga acccgaatgc ccgaatgagg cgatttcaat gggagatcat
     3421 atctacgaga ttaacagcga taagtgtacc gaatgcgtag ggcactacga gacaccaacc
     3481 tgccagaagg tgtgcccgat ccccaatact attgtgaaag atccggcgca tgtcgagaca
     3541 gaagaacagt tgtgggataa atttgtgctg atgcaccacg cggataaaat ttaatattct
     3601 actctggaag tagagtatta attatattac tgggaagcct taacgccatt atatttattt
     3661 aattgatgac attagcataa tcattcacta agttaattta tatagtatct gccaagacac
     3721 ttatttatag ttattaaagg cgcgtccgat tggttcaccg gacgcacctt aagtacgttt
     3781 ccttgtgtta taagaacaga aggatcagct gtaaaacagc aatgatgatt ttgatgaccc
     3841 gtttaatcag gtatcggcaa tcagtcattc gtttttcctt aaacaaggaa tgcagccgtt
     3901 cagattagcc cttacatctc cccaaaactg aacgtgcgag ttattgaggg tgcatgctgc
     3961 actccacacc agagctttga cgacaccact cgtttcaatg ggggaattct gtggcatggt
     4021 gtaaagcaca gcaaaatctt caataacgaa gccaatttta atgtacttac gaattggcgg
     4081 tcacgttaat ctttccatca atattgcttt cttcgtaaag gctcgagttt ttatgctaaa
     4141 gattgcaagt tgcttgtaaa agataagtac actgatccat aatcgctgtt gttgagggtg
     4201 catgctgcac aaaattaaag ttaaaaagta aaacccccgt tccttaccag ttcgggggtt
     4261 ttacttttta aagagaacgg tattatttta actttcaata attaccgtgg cacaagcata
     4321 gtggcgttca tctgccagcg ttacatgcat atttacaacg ccgagctttt ccgccagttt
     4381 taatgcctca ccccatagcc gtagccgtgg tttgccgagc tcatcattga atacttcaaa
     4441 ttgattaaac gccagaccat tgcggatccc ggtgccaaac gcttttgctg cggcttcttt
     4501 cacagcaaaa cgcttcgcca gaaaacgcac cggctggtgg tgcgttttcc agattgccca
     4561 ttcgttatcg cttaatacgc ggcgtgccag gcgctcaccg gatcgggcga tcactgcttc
     4621 tatgcgagcg atctctacga tatccgtgcc taaacctaat attgccatta gccacgcgct
     4681 tccagcatca gacgcttcat ttctgccacc gcatctttca gtccggtcat cactgcacga
     4741 ccaataatgg catgaccgat attcagttca tgcatctcag ggatggcggc aatagctttc
     4801 acgttgtgat aggtcagacc gtgaccagcg ttaactttca gaccgagact agcggcaaag
     4861 gtcgcggctt tggcgatacg cgccagctct tgcgcctgtt cggcgtcagt tttggcatca
     4921 gcatagcaac cggtgtggat ctcgataaac ggtgcgccaa cctctgccgc agctttgatc
     4981 tgctcttcat cggcgtcgat aaagagagaa acctggatcc cggcatctgc cagacgtttg
     5041 caggcatcgc gcattttgtc acgctgccct gcgacgtcca ggccgccttc ggttgttact
     5101 tcctgacgct tttccggtac cagacagcaa aaatgtggct tcgtctcaac ggcgatcgcc
     5161 agcatctctt cggtcaccgc catctccaga ttcatgcggg tatccagtgt ctgacgcagg
     5221 atgcgcacat cacggtcagt aatgtggcgg cgatcttcac gtaaatgcac ggtaatgccg
     5281 tccgctcccg cctgctcagc aataaacgcc gcctgcaccg gatccgggta agcagtaccg
     5341 cgtgcgttac gcagcgttgc aatatggtca atgttgacgc ctaacagtaa ttcagccatg
     5401 acaatcctca tcattcataa tgtgttttca ccgttcgctt aggcataaac tgccggaaca
     5461 gttccctgct ctttaatggt ttaccgccaa gatacggctt aagcgccatg cgggtaaagc
     5521 gtttcgcggc gcgtagtgtg tctgcgtcag gaaattcccg cgcgtttaac gcttttaact
     5581 gccttccggt gaacgtttta ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc
     5641 gataacgata cgtcatggtg tcatctaccg gctcgccgct acccgcacaa tgggtaaaat
     5701 tgacgccata gcccagatgc ccgagcagtg ccagttcaaa gcggcgcagc gcgggttctg
     5761 gcgtaccagt ggcacctgca agagactgaa tgcagtgcaa gtaatcgaaa aagagttcag
     5821 agaagcgcgt ctcgtattcc agtacgcggg agagaagttc gttgatgtac agaccgctgt
     5881 aaagcgtgat accgcttaat ggcagcgcca gcgagacagc ttcagcactg cgcagcgttt
     5941 tgacttcgcc acgcccgccg aaacgtagca agagaggggt gaaaggctgt aatgcacctt
     6001 tcagagtaga gcgtttagag cgtgcgcctt tggcaaccag acgcacgcga cccgattcct
     6061 ccgtgaagac gtccagcatc aggctggttt cgctccacgg gcgactatgc aggacaaatg
     6121 cgcgctgcca gccttccatc ggagtgactc ttaaagatcg tcaacgtaac cgagactgcg
     6181 cagtgcgcgt tcgtcgtcgg cccaaccgga tttcactttt acccacagct caaggtggac
     6241 aggcgcttcg aacatttcct gcatgtcttt acgcgcttca atcccgatgg ttttgatctt
     6301 ggcccctttg ttgccaataa ccatcttctt ctgcccttca cgctcaacga gaatcaaacc
     6361 attgatgtca taaccaccgc gttcgttaga gacgaaacgt tcgatctcca cggtcacgga
     6421 gtatggcagt tcagcgccga ggaaacgcat cagtttttcg cggatgattt cagacgccat
     6481 aaaacgctgt gagcgatcgg tgatgtaatc ttccgggaag tgatgggtcg cttcagggag
     6541 gtgcttacgc acgattgctg cgatagtgtc gacattcagc ccggtttcag cagagatcgg
     6601 cacgatatcg aggaagttca tctgacttgc caggaactgt aagtgcggca gcagatcggc
     6661 tttttcctgc acgttatcca ctttgttcac cgcgaggatc accggcgctt tgccatcgcg
     6721 cagtttgttg agcaccattt cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat
     6781 caccagctca acgtcgccaa tagagctgct cgccgctttg ttcatcaggc ggttaatagc
     6841 gcgtttttct tccatatgca ggccaggggt atcaacgtag atcgcctgat acgcgccttc
     6901 agtatggatc cccacaatgc ggtgacgagt tgtttgcgct ttgcgggagg tgatggagat
     6961 cttctgcccc agcagtttgt tcaacaatgt ggatttgcca acgttcggac gtccgacgat
     7021 ggcaataaat ccgcagtaac ttttatcgat gctcattcca gctccagttt tttcaacgcc
     7081 tgttcggcgg cagcctgctc agccttacga cggcttgaac ctgtgccaac caccggttca
     7141 ctcaggccgc tgacctggca gtggatagta aattcctgat cgtgcgcttc gccacgtacc
     7201 tggactacca gataagtcgg cagcggcaga tggcgaccct gcaaatattc ttgcaagcgc
     7261 gttttcggat ctttttgttt atcgcctggg ctaatttcgt ccaaacgagt ttgataccag
     7321 ttgaggatta atttctcgac ggtttgaata tcactgtcga ggaatacgcc accaattaat
     7381 gcttcgacgg tgtcggcgag aattgactca cgacgaaatc caccgctttt aagttcacct
     7441 ggccctaaac gtaagcactc gcctaactca aattcgcgcg ccagttccgc cagcgtattg
     7501 ccacggacca gcgtggcgcg catccggctc atatcgcctt catccacacg agggaaacgg
     7561 tgataaagcg cattggcgat aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc
     7621 tcgttatgtt tactgctggc actacgatga gttaatgcct gctgcaacag ttcctgatga
     7681 ttaaaagtgt agcccagctt ccgttgaagc cgattaatta cgatggggtt catgcgatac
     7741 caataaataa atgcgtcaac aattcagcac acgaaacaga cctgatatac atggctctgc
     7801 taactgcttc gctgcagttt cagtttaccg gtatatgggg accaacgctg tttcgtgtgc
     7861 cgtggcaacc tggaggtgcc aaccttaaac ttcgggggaa tattctatac acaacgacgg
     7921 gggatgtcgt tagccacggg agatttatct cataaataat tcacgttgtc gccataacgg
     7981 cgacaacgtg aacgaagatg agctattaat ggatgccgcc aatgcgactt aagcgcacac
     8041 cagtcggcca ttcgccttct tgcttatcga agctcatcca gatagccgtt gcccgaccga
     8101 ccagattcgc ttcaggcaca aacccccaat aacggctgtc cgcgctgttg tcgcggttgt
     8161 cgcccatcat gaagtattgt cccggaggaa caatccaggt tgccagttgt tgccctggct
     8221 gctggtaata catccccacc tgatcctgcg caatcggcac tgtcagaatt cggtgcgtca
     8281 catcacccag tgtctcttta cgctcggaaa gacgaattcc attttctttg gtttcgtttt
     8341 tcggcacttc aaagaatccg ctggtcgctt ccccaccatt acggcgtgag aaggtctgaa
     8401 cgaaatcgct cggttccacg tttgagtagg tgaccggcag cgcgttttca cacgcctggc
     8461 cggaactgca tcccggttga atcgtcagct ctttcgagac cggatcataa gtaactttat
     8521 cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt tggatcttcc ggatatttaa
     8581 agaccacgat atctccgcgt ttcggatgac cggtttcgat cagcgttttc tggtagatag
     8641 gatctttaat gccataagca aacttttcta ccagaataaa atcaccgatt aacagagtcg
     8701 gcatcatcga acctgacggg atctggaacg gttcataaat aaacgaacgc acaatcaata
     8761 cgatagccag taccggaaaa acagaagcac cggtttccag ccagccaggc ttcggcgcaa
     8821 cctttttcaa cgttgcttta tccagtgagt caccggcagc cgcctgcgcc gctgcctgac
     8881 gttcccgccg tttaggtgcg aaaaagaatt tatccacgca ccataaaatg cccgtcacca
     8941 gtgtggcaat caccagaatc agggcaaaca tattcgccat gccaactcct aagggttatt
     9001 tgttgtcttt gccgacgtgc agaatggcga ggaacgcttc ctgcggcagc tcgacgttac
     9061 cgatctgctt catgcgtttc ttaccttctt tctgcttctg cagcagcttt ttcttacggc
     9121 tgatatcgcc gccataacat ttagccagta cgtttttacg cagctgtttc acggtggatc
     9181 gcgcaataat gtgcgtacca atcgctgcct gaatggcgat atcaaactgc tggcgtggga
     9241 tcagatcttt catcttctcc accaactcgc gaccgcggtt ttgcgaatta tcacggtggg
     9301 tgatcagcgc caacgcatca acacgttcac cgttgattaa tacgtctaca cgtaccatgt
     9361 cggacgcctg gaagcgtttg aagttgtaat ccagagacgc ataaccacgc gaggtagatt
     9421 tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg gatctcgtac gtcagcgcca
     9481 cctgattacc gtggtaaacc atattggtct gcacgccgcg tttttctacg cacagcgtaa
     9541 taacgttgcc gagatatgcc tgcggcagca gcatgtgaca ttcggcaatc ggctcgcgca
     9601 gttcgtagat gttatttacc gctggcagct tggatggact gtcgacgtaa ataacttctc
     9661 ttgacgtggt ttcaacttca tacactacgg tcggcgcagt ggtgatcaga tccagatcgt
     9721 attcacgttc cagacgttcc tggatgatct ccatgtgcag caggccgagg aagccgcagc
     9781 ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata gaacagtgaa gcatcgttca
     9841 ggctgagttt acccagcgcg tcacggaagg cttcatagtc gtcggaactt accgggaaca
     9901 gaccggcgta tacctgcggt ttgactttct taaagccagg cagcgccttt tctgccggat
     9961 tacgcgccag cgttaaggta tcgccgactg gagcgccgtg gatatcttta atcgcacata
    10021 ccagccagcc tacttcgcca catttcagtt cagtgcggtc aacctgtttc ggcgtgaaga
    10081 tgcccagacg gtcggcgtta taggtctgcc cggtactcat gactttcact ttgtcgccct
    10141 tacgcagggt gccgttttta atacggataa gtgaaacaac gcccaggtag ttgtcgaacc
    10201 atgagtcgat aattagtgcc tgcaacgggc cttccggatc gccttccggc ggcggaatgt
    10261 cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc ggttttcgct gaacagcgca
    10321 ccgcgtcagt ggcgtcgatg ccgacgatat cttcaatttc ttccgccacg cgttcaggat
    10381 cggctgccgg caggtcaatc ttgttcagta ctggcaccac ttcgagatcc atttccatgg
    10441 cggtgtagca gtttgccagg gtttgcgctt ccacgccctg cccggcgtcg accaccagca
    10501 atgcaccttc acaggcagcc agcgaacggg aaacttcata ggagaagtct acgtggcccg
    10561 gggtgtcgat aaagttaagc tgataggttt cgccgtcaga cgctttgtag tccagcgtca
    10621 cgctttgcgc tttgatggta atgccacgct cacgctcaag atccatggaa tcgagaacct
    10681 gcgcctccat ttcacggtca gacaggccac cgcagatctg gataatacgg tcagacagcg
    10741 tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt acgtatattc ttcataaagt
    10801 atgattattg tgccttaatg cccggttaac caggctttta gaagtcgctg ttctgagctt
    10861 aacgtctgta ttaatagaaa cgccgcattc tacactacaa cattgaggcg aggaaatgtt
    10921 cataccgtat ggattgtggt atctggaaac gtcctcgcat ttgttatgca aaatgcaaca
    10981 aagccagtga aatcactggc tcgcgtcttc cgaagatgtt tcaaatcgca caaggccagg
    11041 cggcaatgcc acgcttaaga tgatcggttg ccattctgcc cgggcagcaa acttacgcga
    11101 gtagccgcgc gcaatcagga acccgccaat accaccgagg atcgcaccac ataatgctgc
    11161 gacgtcagaa gcaaagagta gctgaaatag cgaagcgata aggaataatc ccaccagcgg
    11221 cgacatataa accagtaatg cggagctaag caggctgcct tcggcgatcc ccaattccac
    11281 tttttgcccc ggcactaacg gttcatcaca gggtacgaca atggtatgcg tggtttgcgg
    11341 gccaagttta ttcaacacgc ggctaccgca accggcgcgt gaagcgcagc tgctgcatga
    11401 agctttaaca tcacaactga ccagcgcctg cccgttttgc caggagacga cggtagccca
    11461 ctctttgatc attgcgctgc cccgaactta atattctcgg caatgcgttt cgccgtttgc
    11521 ggcggcagtt caccgacaat ggtgatttcg gcgttatcac gtacgcttgt actgacggtt
    11581 ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg tagcgcggtt aacgtttacc
    11641 gagaagctga ataatccgtc ggaatagaga cgtgattcga taggcatgtt gtccatcgtc
    11701 ggtagcggac gtcgactact ggaaacttcg ctaaaaccct gtggcaacca ggttggcgtc
    11761 cagctgaatt tagctttttc acctacagga acagaaagca acggcggcaa atttgccttc
    11821 gccagcgtct gcatactgct actgatatcc tgattgacgt taaaagcaat cacgcgaaat
    11881 tgttccagcg tttcaccatc gcgatcaagg agatcaaccc tcatcggtaa tttcgattcg
    11941 atgtccatcc acacgatgta gctgtagcgt gtaccatctc gggcaaccac gcgaatgact
    12001 tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga taaagtcgta gtaaggagaa
    12061 aggcgtttga aatcggtata gatgagcgat ggcagagaat caacaatgta atcgccatta
    12121 agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt cattgccgcg ctgtaccact
    12181 tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag gacggttatc gaggcgtgca
    12241 tgtcgataac gcagagactc aacaccctgt ttattgatgc tgatgaatga cagctcgtaa
    12301 ttcagtgact gactggccag gttcatctgc tgtaataacg ccccggacgc gggagtggcc
    12361 gaggcgttag cagagaataa caggctacct gtcactaatg acatggcaaa ccaaagttgc
    12421 ttcattactg cgattgcgtt cctaaagttt gaattcctgg cacctgtaca gcggcttgct
    12481 gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag tcggcgttgc agttcgtaat
    12541 cctgcaacat tgcattaatg cgacgacgct gctcctgtac ctgctgctgt tgaccattgt
    12601 ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct ggctttaccc atcatcggca
    12661 gtgtattaaa taccggcgtt tcgggctgct gggacgtttc agattgtcca ttatagtgct
    12721 ggacgccaac gataactgca agcgatacgc atgctgctac gcccatttgg gtaagctgtg
    12781 ccgcccacgg acgtactttc tgccagaatg gcattttctg ccattgatgc ggcgcaggct
    12841 gggcttccgg gatcaatgtc gccggttgac gtactggctc ttcttcaatg gcggccatca
    12901 cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc accccgcatt gagtcacgga
    12961 ttaagtgata gctttcccag gttttctgca tttctgggtt atgagccagt tcgttaagca
    13021 gctcactatc cagcgtttcg ccatccatta aagcggaaag ttgttctttc tgcatgccta
    13081 atacccttat ccagtatccc gctatcgtca acgcctgata agcggttgaa ctttgttatc
    13141 aatagcttcc ctcgctcgga agatacgtga acgcaccgta cctaccggac aatccatgat
    13201 agcggctatc tcttcatagc tcaggccatc cagctcccgc aaggttattg ccatgcgcaa
    13261 atcttccggg agggactcaa tagttcggaa aactatctgt ctcagttctt ctgacaacat
    13321 taagttctca gggttcgaaa tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc
    13381 aatggcatcc acatcactgg aaggtggacg acgcccctga gcaaccaggt aatttttcgc
    13441 tgtatttaca gcaatccgat acagccatgt ataaaaagcg ctatctcccc ggaacgaatc
    13501 cagcgcacga taggctttaa taaaagcttc ttgtaccaca tcgggaacat cacccgacgg
    13561 cacatagcgg gaaaccagac tcgccacttt atgctgatag cgcactacca gtaggttaaa
    13621 ggctttctga tctcccttct ggacccgttc aaccaggacc tggtccgtta actgctcgct
    13681 catccgaggt aaagtctccc caaaccaaat ttccacgcgc tatcgaaacg ccactccatt
    13741 agctgcaatt tgagcaagca aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc
    13801 atctgttttt gtttgtcatg ctgtagacgg atcattatct atcattataa gtctacagaa
    13861 tctgaacatc gcattatctg tgtagaaatg cccatttaac tgcctgaaga gtaacccaac
    13921 ggccttttta tttcaccacc taatcctcca ccagccagta acttctcttt ttctcgccgc
    13981 cctgcgtcag cgtgtttagc cactgtaaca aatattaaaa tagcaggtgt ttattcgcac
    14041 aacatgatgc tatgctgacc aaacggtgtt tagtaaatta aacaaagaaa atgaatactc
    14101 tcccagaaca ttcatgtgac gtgttgatta tcggtagcgg cgcagccgga ctttcactgg
    14161 cgctacgcct ggctgaccag catcaggtca tcgttctaag taaaggcccg gtaacggaag
    14221 gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt tgatgaaact gacagcattg
    14281 actcgcatgt ggaagacaca ttgattgccg gggctggtat ttgcgatcgc catgcagttg
    14341 aatttgtcgc cagcaatgca cgatcctgtg tgcaatggtt aattgaccaa ggggttttgt
    14401 ttgataccca cgttcaaccg aatggcgaag aaagctacca tctgacccgt gaaggtggac
    14461 atagtcaccg tcgtattctt catgccgccg acgccaccgg tagagaagta caatccacgc
    14521 tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct ggagcgcagc aacgcagttg
    14581 atctgattgt ttctgacaaa attggcctcc cgggcacgcg acgggttgtt ggcgcgtggg
    14641 tatggaaccg aaataaagaa acggtggaaa cctgccacgc aaaagcagtg gtactggcaa
    14701 ccggcggtgc gtcgaaggtt tatcagtaca ccaccaatcc ggatatttct tctggcgatg
    14761 gcattgctat ggcgtggcgc gcaggctgtc gggttgccaa tctcgaattt aatcagttcc
    14821 accctaccgc actgtatcac ccacaggcac gcaatttcct gttaacggaa gcactgcgcg
    14881 gcgaaggcgc ttatctcaag cgcccggatg gcacgcgttt tatgcccgat tttgatgagc
    14941 gcggcgaact ggccccgcgc gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc
    15001 tcggcgcaga ttgtatgttc ctcgacatca gccataagcc cgccgatttt attcgccagc
    15061 atttcccgat gatttatgaa aaattgctcg ggctggggat tgatctcaca caagaaccgg
    15121 taccgattgt gcctgctgca cattatacct gcggtggtgt aatggttgat gatcatgggc
    15181 gtacggacgt cgagggcttg tatgccattg gcgaggtgag ttataccggc ttacacggcg
    15241 ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt ctatggctgg tcggcggcag
    15301 aagatatcac cagacgtatg cctgatgccc acggcgtcag tacgttaccg ccgtgggatg
    15361 aaagccgcgt tgagaaccct gacgaacggg tagtaattca gcataactgg cacgagctac
    15421 ggctgtttat gtgggattac gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc
    15481 tgcggcggat aaccatgctc caacaagaaa tagacgaata ttacgcccat ttccgcgtct
    15541 caaataattt gctggagctg cgtaatctgg tacaggttgc cgagttgatt gttcgctgcg
    15601 cgatgatgcg caaagaaagc cgggggctgc attttacgct ggattacccg gaactgctca
    15661 cgcattccgg tccatcgatc ctttcccccg gcaatcatta cataaacaga taaaaagcct
    15721 gggtcagcgc cgtatacgct tcggaatagt tctggtctgg cccacgaatg actaagcgat
    15781 cgctaaagca ttctcccgcc tgcggggaga atgccagcag cacccgatgc ggcagtcgcg
    15841 cttcgttttc cgccacatcc gtccgcaaac gtaaatgcca gcccatgctt aatgccagtt
    15901 cagtaaaacc attaccaatc tgctctggca gcaccacgca gaaaaatccc tcttcggtaa
    15961 tgcactccgc cgcacaggtc agcagcgatg ggtgatcaag cgtagtggta tagcgagcct
    16021 gttcccgttg aggtgtcgcg cactctactc cctgctgata gtaaggtggg ttactgatga
    16081 ttaaatcgaa gcgtactgtc tgctgtgtga tccactgctg aatatccgcc gtatggacgt
    16141 taatccgctc tgcccacggg gactggttga tattttcctg cgcctgcgcg gcagcttcac
    16201 tttccagctc aacggcatca attatcacgc tgtcactggt tcgctgcgcc agcatcagtg
    16261 ccagcaaccc gctacccgcg ccgatatcaa ggcaacgttt caccccagcc accggtgccc
    16321 atgcgcccag caaaatgcca tccgttccca ctttcatcgc acagcgatcg tgagcaacaa
    16381 aaaactgttt aaaagtaaat ccattacgac gaagcacgga tgtagactgt gacatgaaaa
    16441 taaaaccttg caggaaaaac ggcgatagca ccgggtgaga acaatacccg agaagcgata
    16501 tccatacaaa cagatgaaga ttgcagccgt aacgtctata atcagcgccc cacacagagg
    16561 tagaacatga ctgtaacgac tttttccgaa cttgaactcg acgaaagcct gctggaagcc
    16621 ctccaggata aaggtttcac tcgcccgacc gccattcagg ctgccgccat tccgcctgcg
    16681 ctcgatggcc gtgatgtact cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat
    16741 ctgctgccag cgttgcagca cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt
    16801 attttgatcc tcaccccaac tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa
    16861 ctggcgaaac atacgcatct ggatatcgcc accatcaccg gcggcgtagc ctatatgaac
    16921 cacgcggaag tgttcagcga aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg
    16981 caatacataa aagaagagaa cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa
    17041 gcagaccgta tgctggatat gggcttcgct caggatatcg aacatattgc tggcgaaacg
    17101 cgctggcgta aacagaccct gctcttttcg gcaacgctgg aaggcgatgc gattcaggac
    17161 tttgccgagc gtctgctgga agatccggtg gaggtttctg ccaatccgtc cacccgtgag
    17221 cgcaaaaaaa ttcatcagtg gtattaccgc gccgatgacc tggaacataa aaccgcattg
    17281 ctggtgcatc tgttaaaaca gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt
    17341 gaacgtgtgc atgagctggc aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc
    17401 gaaggtgaga tggtacaagg caagcgtaac gaagcgatca agcgtttgac cgaaggtcgc
    17461 gtaaacgtac tggttgcaac cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc
    17521 cacgtcttta acttcgatat gccgcgcagt ggcgatactt atttgcaccg tatcggacgt
    17581 accgcgcgcg ccggtcgtaa aggcaccgca atttcgctgg tggaagccca tgaccatttg
    17641 ctgctgggca aggttggccg ttacattgaa gagccgatta aagctcgcgt tattgatgag
    17701 ttacgcccga aaacgcgtgc gccaagcgag aagcagaccg gcaagccatc gaagaaagta
    17761 ctggcaaaac gcgccgagaa gaaaaaagct aaagagaaag agaaaccgcg tgtgaaaaaa
    17821 cgccatcgcg acaccaaaaa tattggtaag cgccgtaaac caagcggaac gggcgtgcca
    17881 ccgcaaacga cagaagagta atctcaatgc cgggtttaag cctggtatta aaagtgctgg
    17941 caaacgcaaa actgcctgat gcgctacgct tatcagtatc tacgtggctc atgcaatata
    18001 ttgaatttgc acgatcttgt aggccggata aggcgttcac gccgcatccg gcatgaataa
    18061 agcgcacttt gtcgacaagt taaatgccgg gtttaatccc gacattattt ttatccagcc
    18121 acaaaactct cttcaacaca ctgaataaaa gtgtgcatcg ccgggctaac cgcttttcca
    18181 gcatggtgtg cgcacatggc cgtaatggtc tgcgactgtt cgccaaaagg caattcaatt
    18241 aattctccgc tctcaagctc tttttccacc gcaaagcggg gcaagtaact gacaccgata
    18301 ttcgccgcga cacaacgttt gatactttca atgcttaaca gctcaatggt gttttccacc
    18361 gtgatccgcc gctgacgcag cgtgctctca aatatctgcc ggaagacaca ttgcggttcg
    18421 ttgataataa agctacaggc gttatgtctt cccggctcag taaaatcgac atctgcaatt
    18481 tgcggtgaag ccaccagcac cagcgattgt tcacccaact ctcgtcggtt cagggcatca
    18541 tcattcccta cgcgataaaa gacgccgaca tcggcctcat cattcagtag cgcatcacgg
    18601 atcacgtaac agttcagcga ctgcaacgat aaacgcactt ttggtgcgcg ttgccgaaaa
    18661 cgctgcaaca cctgcggcat tcggtaagag agcaacgttt cgcccgaaac aacgcgtagt
    18721 tccccgtccg gatctgactc ctttttggca gcctcacgaa gcgtatccat cactcgggta
    18781 agttcataaa tgtgcggcag caactttttt ccttcgcggg taaggcacat ccgtcggcca
    18841 attttctcaa ataactggac tgaaaactcc tgctcaagct gctgaatatg aaaagtcacc
    18901 gtcgattgtg tacagcacaa tttttgcgaa gctcgcaaaa aggaaccctc ttccaccacg
    18961 gttttaagcg taataaaacg gcgcagatcc ataaccccaa acctatcgaa aatatcgaat
    19021 ctagaatata aaaacattca tttttttaaa tgttccgtgt cgggtactgt ctaccaaaac
    19081 agaggagata acaagtgaca ccgacccttt taagtgcttt ttggacttac accctgatta
    19141 ccgctatgac gccaggaccg aacaatattc tcgcccttag ctctgctacg tcgcatggat
    19201 ttcgtcaaag tacccgcgtg ctggcaggga tgagtctggg atttttgatt gtgatgttac
    19261 tgtgtgcggg catttcattt tcactggcag tgattgaccc ggcagcggta caccttttga
    19321 gttgggcggg ggcggcatat attgtctggc tggcgtggaa aatcgccacc agcccaacaa
    19381 aggaagacgg acttcaggca aaaccaatca gcttttgggc cagctttgct ttgcagtttg
    19441 tgaacgtcaa aatcattttg tacggtgtta cggcactgtc gacgtttgtt ctgccgcaaa
    19501 cacaggcgct aagctgggta gttggcgtca gcgttttgct ggcgatgatt gggacgtttg
    19561 gcaatgtgtg ctgggcgctg gcggggcatc tgtttcagcg attgtttcgc cagtatggtc
    19621 gccagttaaa tatcgtgctt gccctgttgc tggtctattg cgcggtacgc attttctatt
    19681 aacgaaaaaa agcggaagag gtcgccctct tccgcttagt aacttgctac ttaagcctta
    19741 caggctttca gtaaaggtac gagcgataac gtcgcgctgc tgttccggag tcagagagtt
    19801 aaagcgaact gcataaccgg atacacggat ggtcagctgc ggatattttt ccggatgctt
    19861 aactgcatct tccagagttt cacgacgcag aacgttaacg ttcaggtgtt gaccaccttc
    19921 aacgcgaact tctggtttca cttctactgg aacttcacgg tattcaatgt cacccagttt
    19981 gcttactgca accacttcat cttctgcata acctgctttt gcaacgatgc aacgtgcttc
    20041 gcctttttcg ctgtccagca gccagaaaga gttcagcaga tcgtcgttag cggctttagt
    20101 aatctggata cctgtaatca tgtgatgcct ccccggcaaa attatttgat ttgttcagcc
    20161 tgtcgcggcc aattggtaaa accattgttg cttgagtgta tatatactcc tcaaccaccc
    20221 ttgattcttt gatttaaatc aataaaaacc acacatcgat tgtggtcgta aatggatttt
    20281 attgttttac atcaacttat gcgggtgtga aattttacca atttacattt ttttgcactc
    20341 gtttaagtct aaaaaatgag catgattttg ttctgtagaa agaagcagtt aagctaggcg
    20401 gattgaagat tcgcaggaga gcgagatggc taacgaatta acctggcatg acgtgctggc
    20461 tgaagagaag caacaacctt attttcttaa tacccttcag accgtcgcca gcgagcggca
    20521 gtccggcgtc actatctatc caccacaaaa agatgtcttt aacgcgttcc gctttaccga
    20581 gttgggtgac gttaaagtgg tgattctcgg ccaggatcct tatcacggac cgggacaggc
    20641 gcatggtctg gcattttccg ttcgtcccgg cattgccatt cctccgtcat tattgaatat
    20701 gtataaagag ctggaaaata ctattccggg cttcacccgc cctaatcatg gctatctcga
    20761 aagctgggcg cgtcagggcg ttctgctgct caatactgtg ttgacggttc gcgcaggtca
    20821 ggcgcattcc cacgccagcc tcggttggga gacgtttact gataaagtca tcagcctgat
    20881 taaccagcac cgcgaaggcg tggtgttttt gttgtgggga tcacatgcgc aaaagaaagg
    20941 ggcgattata gataagcaac gccatcatgt actgaaagca ccgcatccgt cgccgctttc
    21001 ggcgcatcgt ggattctttg gctgcaacca ttttgtgctg gcaaatcagt ggctggaaca
    21061 acgtggcgag acgccgattg actggatgcc agtattaccg gcagagagtg agtaaatttg
    21121 cggtaaaatg ccggatggca gagttgccac ccggctgatt tatcaggctt tattctgacg
    21181 ccaccattca ccaagcaaaa cgccggttgc aacagagata ttcagcccgg caacgttgcc
    21241 cgtaccgtca atcttcacgc gcagatcgtt cggatcgcgt gcggcatccg gtaacccttc
    21301 atattcctga cccagcacca gtaccatttt cgctggcaga ctggttttga acagcggttt
    21361 cccctgctcg ctggaagtgg tcactacggt gtaacctgcc tgacggaaat catccaacac
    21421 gttaacaatg ttgtcgccag taatcggctg aacgtgctct gcgccgcctt ctgcggtacg
    21481 gatagccgcc cccgactcca gcagtgccgc atcctgcacc acaacgcctt tcacgccgaa
    21541 gtgcgcgcag ctgcgcatca tgccacccag gttatgcggg ttagattcgt tttccagcgc
    21601 cagtacacaa tcctgcgcgc ctgcctggct tacccactgc tgcacggttg tgccattacg
    21661 ctttttgatc aggaagcaaa cgccgccgtg atgttccgtg cctgacgctt ttgtcaattc
    21721 cgcttcatcc accacatggt acgctttgcg gtttgctgcc atccagcgca aggcttcttt
    21781 aaaacgcggc gttacactct ggataaacca ggcgcgaaca atcgcttccg gacggctctg
    21841 gaaaagtgcc tgacatgcat tttcgccgta gacgcgggtt tcttccgcac gctgacgacg
    21901 caacacttcc ggatcaataa aacttttacc actgatgcca ccgtgatcgg ccttttccgg
    21961 cgtctcatca cccggcgcgc gggaaaccgt gcgccacggc gaatcttccc acttgcggtc
    22021 acggggctga ctctgtttgt catcgcgggc ggggcgacgg ccaccgtcag cacgagattt
    22081 tcctggacgc ccgccccctt tcccggtacg cgggttgtgg gtacgtttat cagaatcatc
    22141 atcactgcgg acatacatca ctttgacctt gccgctttta cctttcattt catcgttcat
    22201 gcttttctcc accagcgctg cgcgaagcgc gcagattacc cgaagtccgc gcggttcgcc
    22261 atgatttcgt accaaagcct gcgactatca tacctattga ataaaacaga ttgttgtctg
    22321 gaacaatgtc cccgataata tgtaacatat tagaaacata ccggcgtcgt tgccgataag
    22381 tctccttact catcccgagg ttagttatga ataccgtttg tacccattgt caggccatca
    22441 atcgcattcc cgacgatcgg atcgaagatg cggcaaaatg cggacgctgc ggtcacgact
    22501 tgtttgacgg agaggtgatt aatgcgaccg gtgaaacact cgacaaattg ctgaaggatg
    22561 atctacctgt ggtgatcgac ttctgggcac cgtggtgcgg cccctgccgt aatttcgcac
    22621 caatttttga agatgtcgcg caagagcgta gcggtaaagt gcgctttgtg aaagtgaata
    22681 ccgaagctga acgtgaattg agcagtcgct ttggaattcg tagtataccg acgatcatga
    22741 ttttcaaaaa cggtcaggtt gtcgacatgc ttaatggcgc agtaccgaaa gcgccgttcg
    22801 atagctggct gaacgaatct ctttaatctt accggggcgc atcttgtgcc ccgttttctc
    22861 ctctgcgaca atggcgtttt ttcgacgctc tcttatgacc gaaaacgctg ttctccagtt
    22921 acgcgccgag cgtattgcgc gcgcaacacg tccttttctt gcccgcggta atcgcgttcg
    22981 tcgctgccaa cgctgtcttt tgccagagaa attatgtctc tgttcgacaa ttaccccagc
    23041 acaagcaaaa agtcgcttct gtttgctgat gttcgacacc gagccaatga agcccagtaa
    23101 taccgggcgt ctgattgctg atattttgcc tgataccgtt gcgtttcaat ggtcgcgtac
    23161 cgaaccctcg caggatttgc tggagttggt gcaaaacccg gactatcagc caatggtggt
    23221 ctttcccgct tcgtatgctg atgagcaacg ggaagtgatc ttcacaccac ctgccggtaa
    23281 gccaccgctg tttatcatgc tcgatggtac ctggccggaa gctcgcaaga tgtttcgtaa
    23341 aagtccgtat ctggataatc ttcccgtcat ttccgtcgat ctttcccggc tttctgccta
    23401 tcgcctgcgt gaagcccagg ctgcaggcca atattgtact gccgaggtag ccatcgcact
    23461 gttagatatg gccagcgata ccggggcggc agcagggtta ggcgagcatt ttacccgctt
    23521 taaaacacgc tatctggcag gaaaaacgca acatctgggt agcatcacag cagaacagtt
    23581 agaaagcgtt taaaatcatt cggtcacttc tgcgggagac cggtatgagt cagcgaggac
    23641 tggaagcact actgcgacca aaatcgatag cggtaattgg cgcgtcgatg aaacccaatc
    23701 gcgcaggtta cctgatgatg cgtaacctgc tggcgggagg ctttaacgga ccggtactcc
    23761 cggtgacgcc agcctggaaa gcggtgttgg gtgtgttggc ctggccggat attgccagct
    23821 tgccctttac acccgacctt gcggttttat gtaccaatgc cagccgtaat cttgctcttc
    23881 tggaagagct cggcgagaaa ggctgtaaaa cctgcattat tctttccgcc ccggcatcgc
    23941 aacacgaaga tctccgcgcc tgcgccctgc gccataacat gcgcctgctt ggaccaaaca
    24001 gtctgggttt actggctccc tggcaaggtc tgaatgccag cttttcgcct gtgccgatta
    24061 aacgcggcaa gctggcgttt atttcacaat cggcagccgt ctccaacacc atcctcgact
    24121 gggcgcaaca gcgtgagatg ggcttttcct actttattgc gctcggcgac agcctggata
    24181 tcgacgttga tgaattgctc gactatctgg cgcgcgacag taaaaccagc gccatcctgc
    24241 tctatctcga acagttaagc gacgcgcgac gctttgtttc ggcagcccgt agtgcctcgc
    24301 gtaataaacc gattctggtg attaaaagcg gacgtagccc ggcggcacag cgactgctca
    24361 acacgacggc aggaatggac ccggcatggg atgcggctat tcagcgtgcc ggtttgttgc
    24421 gggtacagga tacccacgaa ctgttttcgg cggtggaaac ccttagccat atgcgcccgc
    24481 tgcgtggcga ccggttgatg attatcagca acggtgctgc gcctgccgcg ctggcgctgg
    24541 atgccttatg gtcacgcaat ggcaagctgg caacgctaag cgaagaaacc tgccagaaac
    24601 tgcgcgatgc actgccagga catgtggcaa tctctaaccc gctcgatctg cgcgatgacg
    24661 ccagcagtga gcactatatt aaaacgctgg acattctgct ccacagccag gattttgatg
    24721 cgctgatggt tattcattcg cccagcgccg ctgctcccgc aacagaaagc gcgcaagtat
    24781 taattgaagc ggtaaagcat catccccgca gcaagtatgt ttctctgctg acgaactggt
    24841 gtggcgaaca ttcctcgcaa gaggcacgac gattattcag cgaagccggg ctgccgacct
    24901 accgtacccc ggaaggaacc atcactgctt ttatgcatat ggtggagtac cggcgtaatc
    24961 agaagcaact acgcgaaacg ccggcgttgc ccagcaatct gacttccaat accgcagaag
    25021 cgcatcttct gttgcaacag gcgattgccg aaggggctac gtcgctcgat acccatgaag
    25081 ttcagcccat cctgcaagcg tatggcatga acacgctccc tacctggatt gccagcgata
    25141 gcaccgaagc ggtgcatatt gccgaacaga ttggttatcc ggtggcgctg aaattgcgtt
    25201 cgccggatat tccacataaa tcggaagttc agggcgtcat gctttacctg cgtacagcca
    25261 atgaagtcca gcaagcggcg aacgctattt tcgatcgcgt aaaaatggcc tggccacagg
    25321 cgcgggtcca cggcctgttg gtgcaaagta tggctaaccg tgctggcgct caggagttgc
    25381 gggttgtggt tgagcacgat ccggttttcg ggccgttgat catgctgggt gaaggcggtg
    25441 tggagtggcg tcctgaagat caagccgtcg tcgcactgcc gccgctgaac atgaacctgg
    25501 cccgctatct ggttattcag gggatcaaaa gtaaaaagat tcgtgcgcgc agtgcgctac
    25561 gcccattgga tgttgcaggc ttgagccagc ttctggtgca ggtttccaac ttgattgtcg
    25621 attgcccgga aattcagcgt ctggatattc atcctttgct ggcttctggc agtgaattta
    25681 ccgcgctgga tgtcacgctg gatatctcgc cgtttgaagg cgataacgag agtcggctgg
    25741 cagtgcgccc ttatccgcat cagctggaag aatgggtaga attgaaaaac ggtgaacgct
    25801 gcttgttccg cccgattttg ccagaagatg agccacaact tcagcaattc atttcgcgag
    25861 tcaccaaaga agatctttat taccgctact ttagcgagat caacgaattt acccatgaag
    25921 atttagccaa catgacacag atcgactacg atcgggaaat ggcgtttgta gcggtacgac
    25981 gtattgatca aacggaagag atcctcggcg tcacgcgtgc gatttccgat cctgataaca
    26041 tcgatgccga atttgctgtg ctggttcgct cggatctcaa agggttaggc ttaggtcgac
    26101 gcttaatgga aaagttgatt acctatacgc gagatcacgg actacaacgt ctgaatggta
    26161 ttacgatgcc aaacaatcgt ggcatggtgg cgctagcccg caagctcggg tttaacgttg
    26221 atatccagct cgaagagggg atcgttgggc ttacgctaaa tcttgcccaa cgcgaggaat
    26281 catgagtaag gtactggaaa tgttgaccac ttaatcggga ctggtgttat tattgcccgc
    26341 ttatgtcgtc tgcattgcac agaggaccct tcaatgaaca gagaagaaat gcactgtgat
    26401 gttgtcaaaa tttaagcgta ataaacatca acaacacctt gcccaactac ccaagatttc
    26461 tcaatcagtt gatgatgtcg atttctttta cgctcccgcc gacttccggg agacgctgct
    26521 ggaaaaaata gccagcgcga agcagcgcat ttgtattgtc gccctgtatc tcgaacagga
    26581 tgacggtggc aaaggcattc tgaacgcgtt gtatgaagct aaaaggcagc gtccggaact
    26641 ggatgtgcgg gtgctggtcg actggcatcg tgcacaacgt ggacgcattg gcgctgcggc
    26701 atctaacact aacgctgact ggtactgccg catggcgcag gaaaatccgg gcgtagatgt
    26761 tccggtttat ggcgttccaa tcaatacccg tgaagccctt ggtgttctgc actttaaagg
    26821 ctttatcatc gacgatagcg tactttatag cggtgccagc ctgaacgatg tttacctgca
    26881 tcagcacgat aaatatcgct acgaccgtta tcatctgatc cgtaaccgta agatgtcaga
    26941 cattatgttt gaatgggtta cacagaatat tatgaatggc cgcggcgtta atcgtctgga
    27001 tgatgttaat cggccaaaaa gcccggaaat caagaacgat attcgtctgt tccgccagga
    27061 gctgcgtgat gccgcttatc atttccaggg cgatgccgac aacgatcagc tttccgtaac
    27121 gccgctagtg gggctgggga aatcgagtct gttgaacaag accattttcc atcttatgcc
    27181 ttgtgccgag cagaaactaa ccatctgtac gccatacttc aacctgccag caatccttgt
    27241 gcgcaatatt atccagttgc tgcgcgaagg gaaaaaggtc gaaattattg ttggtgataa
    27301 aaccgcgaac gacttctaca ttccggaaga tgaacctttc aagataattg gcgcattgcc
    27361 ttatctctat gagatcaatc tgcgtcgttt cctgagccgt ttgcagtatt acgtcaatac
    27421 tgaccagcta gtggttcggt tatggaaaga tgacgacaac acctatcacc taaaagggat
    27481 gtgggttgat gataagtgga tgttgatcac cggtaataac ctgaacccgc gcgcctggcg
    27541 tctggatctg gaaaacgcca ttttgatcca cgatccacaa ctggagcttg cgccgcagcg
    27601 agagaaagaa ctggagctga tccgcgagca taccaccatc gttaagcact atcgcgatct
    27661 gcaaagtatt gccgattatc cggtgaaggt tcgtaaactc atccgccgtt tgcgccgtat
    27721 ccgcatcgac cgattaatta gccgcatcct gtaatcacaa ccccgtcctg tacggggttt
    27781 gttttttgga ggccacgttt tgcgtattct ttttgtctgt tcgctattgt tactttctgg
    27841 atgcagccat atggctaacg atagctggag cgggcaggat aaagctcaac actttatcgc
    27901 ctcggcgatg ctttccgccg ccggaaatga atattcacag catcagggga tgagccggga
    27961 tcgcagtgcc atgtttggat tgatgttctc tgtcagtttg ggggcgtcaa aagagctttg
    28021 ggatagccgc cccgaaggga gcggctggag ctggaaggat ttggcctggg atgtcgccgg
    28081 tgcaagcacc ggctataccg tctggcaact gacccgtcac taaagacgca tccccttccc
    28141 tttgcgatgt agcatcaaag aaaccagaaa cgccaccacg gccatcaagg tcacatacca
    28201 gaagaaggct gtttccattc ctattgattt cagcgacaac gctacgtact ccgccgaacc
    28261 accaaatata gcattagcga ccgcatatga cagaccaacg cctaatgcgc gaacctgtgc
    28321 cgggaacatc tcagccttca gtattccact gattgatgta taaaaactca ctatcagcag
    28381 ggcacacatc accagaccaa aagcggcata aggcgaggaa acgttttgca atgctgagag
    28441 aataggaacg gtaaaaatgg ctgccagcga accgaaacat aacattgagg tacggcgacc
    28501 aatcttatcc gacagcgcgc caatgagtgg ttgaataagc atgaatacaa acaatgcggc
    28561 agtcataatg ccactcgcca cgttggcatg cattcccgca gtatttacca gatacttctg
    28621 catataagtg gtgaaggtat agaaacaaag ggagcccgca gcggtaaaac cgagaaccat
    28681 gatgaatgca cggcgattgc gccataatcc tttcagagat ccagcttctt ttaaagcgcg
    28741 cgtttcttgt tgcgaagttt catctaactg acgacgtaac cacaacgcca caacagctaa
    28801 cacagctcct aacgcgaaag gaatacgcca tccccactct ctgagtgcag cgtcttccat
    28861 ggtgtgttgt aaaaccacga caaccagtag ggctagcagt tgtccgccga tcaacgtcac
    28921 atactgaaat gatgcgtaaa aacctttgcg cccttcaacg gcaacttcac tcatataggt
    28981 ggcgctggtg ccatattctc cgccaacgga taatccctga aataaacgag cgagaagcaa
    29041 taatgccgga gcccacgtac ctatagtttc ataacctggg aggcaggcga taaccagcga
    29101 tccgaaacac atcatacaca ccgataacag catcgatttt ttgcgaccat gtttatcggc
    29161 tatgcggcca aatagccaac cgcctattgg gcgcatcagg aatcccgcag caaaaacacc
    29221 tgctgtttgt agtagttgag tcgtcgtgtt cccggaaggg aagaaaatgt gggcgaagta
    29281 gagtgaacag aacgagtaga catagaaatc gaaccactcg accagattac ctgaagaggc
    29341 ccccacaatc gcccaaatgc ggcgacgagt atcactactt gtcagtttgc tgtctgccgt
    29401 tacagtactt tcagccatgc cattatgtct cctgccgtaa tccgatgctt ttgtcggtca
    29461 cttttgttta tttcttttgg taaagaaaat gttatacatt tgttgcatat cattatgcaa
    29521 ccttaaccat gaatttagtt agcaggaaaa tgattattga ggagcttaag gataaatttc
    29581 tggtaaggag gacacgtatg gaagtgagca agttggggaa gccgtattcg ttgctgaatc
    29641 tggcatatgt gggagtataa gacgcgcagc gtcgcatcag gcattttttt ctgcgccaac
    29701 gcaaaaaggc catcgtgact gatggccttt cgcttaattt gatgcctggc agttccctac
    29761 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
    29821 catggggtca ggtgggacca ccgcgcta
//
LOCUS       DAZGCS010000061        29778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGCS010000061 DAZGCS010000000
VERSION     DAZGCS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.061"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..668
                     /gene="zapC"
                     /locus_tag="ACHK01_002807"
     CDS             126..668
                     /gene="zapC"
                     /locus_tag="ACHK01_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO8097736.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(665..1774)
                     /gene="ycbX"
                     /locus_tag="ACHK01_002808"
     CDS             complement(665..1774)
                     /gene="ycbX"
                     /locus_tag="ACHK01_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO8097737.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2018..4126
                     /gene="rlmKL"
                     /locus_tag="ACHK01_002809"
     CDS             2018..4126
                     /gene="rlmKL"
                     /locus_tag="ACHK01_002809"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO8097738.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4137..6044
                     /locus_tag="ACHK01_002810"
     CDS             4137..6044
                     /locus_tag="ACHK01_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8097739.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQSVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6174..7427
                     /gene="pqiA"
                     /locus_tag="ACHK01_002811"
     CDS             6174..7427
                     /gene="pqiA"
                     /locus_tag="ACHK01_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO8097740.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VADLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7432..9072
                     /gene="pqiB"
                     /locus_tag="ACHK01_002812"
     CDS             7432..9072
                     /gene="pqiB"
                     /locus_tag="ACHK01_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO8097741.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9069..9632
                     /gene="pqiC"
                     /locus_tag="ACHK01_002813"
     CDS             9069..9632
                     /gene="pqiC"
                     /locus_tag="ACHK01_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO8097742.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQITVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9888..10055
                     /gene="rmf"
                     /locus_tag="ACHK01_002814"
     CDS             9888..10055
                     /gene="rmf"
                     /locus_tag="ACHK01_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO8097743.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10125..10643)
                     /gene="fabA"
                     /locus_tag="ACHK01_002815"
     CDS             complement(10125..10643)
                     /gene="fabA"
                     /locus_tag="ACHK01_002815"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO8097744.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10712..12472)
                     /locus_tag="ACHK01_002816"
     CDS             complement(10712..12472)
                     /locus_tag="ACHK01_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8097745.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12658..13110
                     /gene="matP"
                     /locus_tag="ACHK01_002817"
     CDS             12658..13110
                     /gene="matP"
                     /locus_tag="ACHK01_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO8097746.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13186..14226)
                     /gene="ompA"
                     /locus_tag="ACHK01_002818"
     CDS             complement(13186..14226)
                     /gene="ompA"
                     /locus_tag="ACHK01_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO8097747.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14583..15092)
                     /gene="sulA"
                     /locus_tag="ACHK01_002819"
     CDS             complement(14583..15092)
                     /gene="sulA"
                     /locus_tag="ACHK01_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO8097748.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15311..15940
                     /gene="sxy"
                     /locus_tag="ACHK01_002820"
     CDS             15311..15940
                     /gene="sxy"
                     /locus_tag="ACHK01_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO8097749.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15903..18065)
                     /gene="yccS"
                     /locus_tag="ACHK01_002821"
     CDS             complement(15903..18065)
                     /gene="yccS"
                     /locus_tag="ACHK01_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO8097750.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18075..18521)
                     /locus_tag="ACHK01_002822"
     CDS             complement(18075..18521)
                     /locus_tag="ACHK01_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO8097751.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18644..20698
                     /gene="helD"
                     /locus_tag="ACHK01_002823"
     CDS             18644..20698
                     /gene="helD"
                     /locus_tag="ACHK01_002823"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO8097752.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20730..21188)
                     /gene="mgsA"
                     /locus_tag="ACHK01_002824"
     CDS             complement(20730..21188)
                     /gene="mgsA"
                     /locus_tag="ACHK01_002824"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO8097753.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21284..21946)
                     /locus_tag="ACHK01_002825"
     CDS             complement(21284..21946)
                     /locus_tag="ACHK01_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO8097754.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22119..22532
                     /locus_tag="ACHK01_002826"
     CDS             22119..22532
                     /locus_tag="ACHK01_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO8097755.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22577..22894)
                     /gene="hspQ"
                     /locus_tag="ACHK01_002827"
     CDS             complement(22577..22894)
                     /gene="hspQ"
                     /locus_tag="ACHK01_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO8097756.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22952..24142)
                     /gene="rlmI"
                     /locus_tag="ACHK01_002828"
     CDS             complement(22952..24142)
                     /gene="rlmI"
                     /locus_tag="ACHK01_002828"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO8097757.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEDGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24237..24515
                     /gene="yccX"
                     /locus_tag="ACHK01_002829"
     CDS             24237..24515
                     /gene="yccX"
                     /locus_tag="ACHK01_002829"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO8097758.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLVQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24512..24841)
                     /gene="tusE"
                     /locus_tag="ACHK01_002830"
     CDS             complement(24512..24841)
                     /gene="tusE"
                     /locus_tag="ACHK01_002830"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO8097759.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAKNEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24932..25591)
                     /gene="yccA"
                     /locus_tag="ACHK01_002831"
     CDS             complement(24932..25591)
                     /gene="yccA"
                     /locus_tag="ACHK01_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO8097760.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25999..27018)
                     /locus_tag="ACHK01_002832"
     CDS             complement(25999..27018)
                     /locus_tag="ACHK01_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8097761.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26996..27238)
                     /locus_tag="ACHK01_002833"
     CDS             complement(26996..27238)
                     /locus_tag="ACHK01_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO8097762.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27306..29777)
                     /locus_tag="ACHK01_002834"
     CDS             complement(27306..29777)
                     /locus_tag="ACHK01_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8097763.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNA
                     LNDARYQAKYVSAIWQKLLPSQADF"
BASE COUNT         7434 a         7246 c         7713 g         7385 t
ORIGIN      
        1 gccctggttg ttttatattc atcgctgttg cttatttaga cattttgtac ttttattgtt
       61 gaggttatta agcgaagcga caatggattg tgttgctgcg gttttatagg gtaggggaga
      121 ggcagatgcg aattaaacca gacgataact ggcgttggta ttacgatgaa gagcacgatc
      181 gaatgatgct cgatttagcc aatggtatgc tatttcgctc acgttttgcg cgcaagatgt
      241 tgactccaga tgccttttct cccgcaggct tttgcgttga cgacgccgcg ctctatttct
      301 cttttgaaga aaagtgccgc gattttaatt tatctaaaga acaaaaagcc gaactggtgc
      361 tgaatgcact ggtagcaatt cgctatctca aaccgcaaat gccgaaaagc tggcattttg
      421 tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg
      481 atactcacga gcaggtcaat ttgctggtcg ttgaatctgg cgaaaatgcc gcactatgcc
      541 tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat gcagttgggc gacgccatta
      601 aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga cagcttcagc ctcgaacagg
      661 cagtctaacg cgccaactta agtgcagtct tcggtacaca gctacagcaa agaatggtgc
      721 catcatcgcc cattgctgat ttttttagcg gcgtgacttc gccttctaaa agctgaacac
      781 ggcaacttcc acaaatgccc gcgcggcaag aataagggat acgaattccc tgattttcta
      841 attgctccag caacacctgt tggttatttc cacgaaatgc ctgtccctgc cagtcaatat
      901 ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt
      961 aaatttttgc cggagccgtt gccagaattt ccacctcatc gccaacgcga atcacgccgc
     1021 tattacgggc aattaaattc tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga
     1081 aagattgcaa tgtttttaat ggttcgcctg ccggatgttt ttgccctttt tctgggctga
     1141 cggtggtgaa aatacagcgg ctacaaggtt taaccacatc aaacaccaca tcaccaatgc
     1201 gaatcacttt ccagctatct tcttcccagg ctgacgcgcc ggaaaccacc agattggggc
     1261 ggaattgctc catttttaca ctggccggac aacgttgttg gagatcacgt aacgaggctt
     1321 cgttagcaag aaggtaagga tagccatcag caaatgacag gggtacagtg ttgtggcgtt
     1381 tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc
     1441 cacttagcca tttgttgatc gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa
     1501 cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg ctactgccat
     1561 ctggtgcggt gagatgcaag ccatcatgca caggtgaagg agtaaaccgt accatctggg
     1621 gaaactggcg agcggtaata aacgtaccgt caggttccgt gatcataaag atgcgatcga
     1681 aggccagacc actgacatca gccagagcat gtgtaagacc aatgccgcgc atcgatttaa
     1741 caggatgaat aaaaagccgg gttaatgtca ccacggtgcg gtcctcaaat gaaaataagc
     1801 cctcaacttt atgacatgac gcgcttatta gctataatgc gcaacaattt tcttagcgcc
     1861 tgtcccatta ggctatttta tttgtcagtt tggccctggg ttgtgctcga aatcctcacg
     1921 tacttgtgta cgctccggtt tctccgcgct gtccatgtcc agactgactg caacaattac
     1981 gcctactgcg gtaggctctt agagtaaaag tgacgatatg aattctctgt ttgccagtac
     2041 ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa aacctggggg ccgttgaatg
     2101 ccaggtggtt cagggtgggg tccatttcaa gggcgacaca cggcttgttt accagagcct
     2161 gatgtggagc cgcctggcct cgcgtattat gttgccgctg ggcgagtgta aggtttacag
     2221 cgatttagac ctctatctcg gtgttcaggc gatcaactgg acagagatgt ttaatcctgg
     2281 cgcgaccttc gctgtccact tcagtggttt gaatgacacc atccgcaaca gtcagtacgg
     2341 cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc
     2401 aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc tggctgcata aagaaaccgc
     2461 cagtatcgct cttgatctga gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc
     2521 tggtattgcg ccgatcaaag aaaccctggc agccgctatt gtgatgcgat ccggctggca
     2581 gccaggaaca ccgctgctcg atccgatgtg tggttccggt acgttgctga ttgaagcggc
     2641 gatgctagcg accgatcgcg caccaggctt gcaccgtggg cgttggggct ttagcggctg
     2701 ggtgcagcat gatgaagata tctggcagga agtgaaagcg gaagcgcaaa ctcgtgcccg
     2761 taaaggcctg gctgagtata gctctcattt ttacggttcg gacagcgacg cacgggtgat
     2821 tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt
     2881 gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg ccgtacggca cagtgttgag
     2941 caacccgcca tacggggaac gtctggacag cgaaccggcg ctgattgcgc tgcatagcct
     3001 gctgggtcgg atcatgaaaa accagtttgg tggctggaat ctctctttgt ttagtgcctc
     3061 gccggatctg ctaagctgct tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg
     3121 cccgctggac tgcgtacaga aaaattacca cgttgccgaa agcactccag acagcaaacc
     3181 ggcgatggta gcggaagact acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa
     3241 gtgggctcgc caggaaggaa ttgaatgtta ccgcctgtat gacgccgatc taccggaata
     3301 taacgttgcc gttgaccgtt atgccgactg ggtggtggtg caggagtatg cgccgccaaa
     3361 aactattgat gctcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc
     3421 ggtactgggg attgcgccaa acaaactggt gctgaaaacc cgtgaacgcc agaagggcaa
     3481 aaatcagtac cagaaactgg gcgagaaggg cgagtttctt gaagttaccg aatataacgc
     3541 tcacttgttg gtgaacctga cggattacct cgataccggt ctgtttctcg atcaccgtat
     3601 cgcccgtcgt atgctcggtc agatgagcaa aggcaaagat ttcctcaacc tgttctctta
     3661 taccggcagc gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga
     3721 tatgtcgcgt acttatctgg agtgggcaga acgcaacctg cgtttgaatg gcctgaccgg
     3781 gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca
     3841 gttcgatctg atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc
     3901 gtttgatgtt cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc
     3961 aggtgggacg attatgttct cgaacaataa acgtggcttc cgtatggatc tcgacggcct
     4021 ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc
     4081 ccgtaaccgc cagatccaca actgctggct gattaccgca gcctaaagga atagtaatgt
     4141 cattaatcag tatgcatggc gcatggctgt cgttcagcga cgcgccgctt ctcgataacg
     4201 cagaactgca tatcgaagat aacgaacgtg tttgtctggt gggccgcaac ggcgcgggca
     4261 aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt
     4321 acgagcaaga tttgattgta gctcgtctgc aacaggatcc gccgcgtaac gttgagggta
     4381 gcgtttatga tttcgttgcc gaaggcattg aagaacaagc ggaatatctg aaacgctatc
     4441 acgatatttc gcgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga
     4501 aggttcagga acagctggat caccacaacc tgtggcagct ggaaaaccgc atcaacgaag
     4561 tgctggcgca actggggtta gatcctaacg ttgcgctgtc gtcgctttcc ggcggctggt
     4621 tgcgtaaagc ggcattagga cgcgcgctgg tgagtaatcc gcgcgtgctg ttgcttgatg
     4681 aaccgacaaa ccacctggat attgaaacca tcgactggct ggaagggttt ttgaaaactt
     4741 tcaacgggac gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc
     4801 gcattgttga tctcgatcgc ggcaagctgg tgacctatcc agggaattac gaccagtacc
     4861 tgctggaaaa agaagaagcc ctgcgcgtgg aagaattaca aaatgccgag ttcgatcgca
     4921 aactggcgca ggaagaggtg tggatccgcc aggggatcaa agcacgccgt acccgtaatg
     4981 aaggccgcgt acgcgccctg aaagcgatgc gtcgcgagcg tggtgaacgt cgcgaagtga
     5041 tgggtaccgc aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa
     5101 tggaagacgt ttgctaccag gttgacggta agcaactggt gaaagatttt tcggcccagg
     5161 ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa accacgctgc
     5221 tgaaactgat gctcggtcag cttcaagcgg acagcgggcg tattcacgtt ggcaccaaac
     5281 tggaagtggc ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg
     5341 ataaccttgc cgaaggtaag caagaggtga tggttaacgg caagccacgc cacgtattgg
     5401 gctatttgca ggactttctg ttccatccga aacgggcgat gacgccggta cgtgcgcttt
     5461 ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat
     5521 tgattcttga cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac
     5581 tgatcgacag ctatcagggc acggtattgc tggttagcca cgatcgtcag tttgtcgata
     5641 acaccgttac agaatgttgg atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg
     5701 gttatcatga tgcccgtggt cagcaagagc agtctgtggc gctcaaacag cctgcggtga
     5761 aaaaaaccga agaagccgcc gcggcaaaag cagaaactgt aaaacgcagc agtagcaaac
     5821 taagctataa attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg
     5881 cgaagctgga agccctacag acgcaggtgg cggatgcttc cttcttcagt cagccacatg
     5941 agcagacaca aaaagtgctc gccgatatgg ctgctgcaga gcaggagctg gagcaagcct
     6001 ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat
     6061 ttaaacagag acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga
     6121 aaaccaacac tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg
     6181 aacatcatca tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac
     6241 cgcgccttga gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg
     6301 cgtgggatgc ccctcggcag cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt
     6361 tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta
     6421 cattactgga aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt
     6481 tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga
     6541 atcgcgcgga attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca
     6601 aaacctgggg aatggcggag attttcctcg ctggtgtgct ggtcagtttc gttaaactga
     6661 tggcttacgg cagcattggg gtaggcagca gctttctccc ctggtgttta ttttgtgtcc
     6721 tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt atgggacgac atcgccccga
     6781 tgccagaact gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt
     6841 cgtgctcctg ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta
     6901 ccaaagggta cgttcggcgt agaaacagcc tgcagtggac actcgcgctg cttgtaacgt
     6961 ccatcatgct gtatcttccg gctaatattt tgcccatcat ggtggcggat ttattaggct
     7021 cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa ggctcttatc
     7081 ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca
     7141 tcgcgtggct gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt
     7201 tgatttatga agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg
     7261 cggtgctctc ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg
     7321 cattaatgtt tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc
     7381 gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct
     7441 aataatgggg aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct
     7501 atcgtcacgg cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg
     7561 gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa
     7621 agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacgcac
     7681 gttgaaatca aagcgcggct gaattccggt atggaaaaat tgctgcataa agacaccgtc
     7741 ttttgggtgg tgaaaccgca gattggtcgc gaagggatta gcggcctggg aacgctgctg
     7801 tctggagttt atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat
     7861 ttgctggact cgccaccgtt ggccccgcct gatgcgaaag gtatccgtgt gattctcgat
     7921 agcaaaaaag ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta
     7981 ggttcggttg aaaccagcac cttcgataca caaaaacgca atatcagcta tcaactgttc
     8041 atcaatgcac cttatgaccg actggtgacc aacaatgttc gcttctggaa agatagtggc
     8101 attgcggttg atctgacgtc agcagggatg cgtgtggaga tgggctcatt gacaacgctg
     8161 ctgagtggcg gtgtcagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca
     8221 ccgaaaacag ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc
     8281 gatcacattg attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct
     8341 ccggtagagt tccggggtat tcgcctgggt accgtaagca aagtgccatt ctttgcgccg
     8401 aatatgcgtc agacatttaa cgatgattac cgtattccgg tactgattcg tatcgagcca
     8461 gagcggctga aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg
     8521 ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc tggtcactgg tgcactgtat
     8581 gttgatctcg atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt
     8641 tatcagatta tcccgaccgt tagcggcggc ctggcgcaaa tccagcaacg actgatggaa
     8701 gcgttggata agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg
     8761 ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga cactggatag catgaacaag
     8821 atcctcgcta gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa
     8881 ttgaatcgca gcatgcaggg cttccagcct ggctccgcag cctacaacaa gatggtggcg
     8941 gatatgcagc gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat
     9001 gagaagagta acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg
     9061 gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct
     9121 ccggcgaaat taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta
     9181 ccgccagcca gggcaatcgt ctgttatggg tagagcagat cactgttcct gactatctgg
     9241 cggggaatgg tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aacaacaact
     9301 tgtgggccag cccgttggat caacagttgc gcaacaccct ggttgccaac ctgagtacgc
     9361 aactgcccgg ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg
     9421 ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc
     9481 tgttgaacca ccagggacaa ctgatcaaac gtccgttccg tctggaagga gtgcaaactc
     9541 aggatggtta cgatgagatg gttaaagtgc tggccggtgt ctggagtcag gaagccgctt
     9601 ctattgcaca agagataaag cgtctacctt aattataaag atttgtaaat ataaccgtct
     9661 ccggtatgtt gcctgaggcg gtttttttgt ctctaacgtg cggaaaaatt tgttcctctt
     9721 cacatttttt gtacaaccga catgcccgtg tagctcacaa atatgacagt ggcgtgaatt
     9781 ttgcgcattg acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta
     9841 atcactgttt tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa
     9901 aacgagatcg cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct
     9961 caaaagaaat gtgtccctat cagacgctga atcaaaggtc acaatggctg ggaggctggc
    10021 gagaagccat ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc
    10081 cgcattgcgg aggtttcgcc ttttgatact ctgtctgatt ataatcagaa ggcagacgta
    10141 tcctggaaca gaccgacttt caggtcgctg gcggtataga tcagacgacc atcaaccagc
    10201 acttcgccat ccgccaggcc cataatcaga cgacggttaa caatgcgttt aaagtgaata
    10261 cggtaggtca cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg
    10321 cccaacgcgc ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc
    10381 cacattgcgt ccaggcccag gcatcccggc ataaccggat cgccaataaa gtggcatccg
    10441 aagaaccaca gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta
    10501 ccacccgttt cggtcatttt gaccacacgg tccatcatca gcatgttcgg tgctggcaat
    10561 tgcgggcctt tagcgccaaa cagttcaccg cgaccagagg caagaaggtc ttcttttgta
    10621 taggattcgc gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga
    10681 tagctaacac gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac
    10741 gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct
    10801 ggatggtttg catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg
    10861 gcagtgcgtc agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt
    10921 tcaccagttc actgtgaaga cttaaatggc gaacgttagc tgtggggata atgacacctt
    10981 gtttcccggt taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta
    11041 aaccaccgac cggctgggcg cgaccgaact gatcgactga acctgtgata gcgatactct
    11101 gattcaccgg cacatcggcg agggcgctta tcagggcgca gagttcagcc atcgaggcac
    11161 tatctccatc aacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct
    11221 gttgctcaag ctgtagttcc gacatcagga acgcttgcat gatcatcatc cctttcgcat
    11281 ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat
    11341 gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa
    11401 tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct
    11461 cctgaaggat ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct
    11521 gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct
    11581 ctttacactg gcggaggatc cactgcgggc taagcggaag tgtttcttgt tcaccggtgt
    11641 agcgtgctgc ttcgcggata agtaccggcc aggcatccgc tcccggtgca ggtaagtgat
    11701 tatgtctggc ggtaaatgtc acccagcgac accactgggt tactgactcc gcatcgacaa
    11761 tctgcagagt atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct
    11821 cctggaaatc agccaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca
    11881 tcgaaggcac agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct
    11941 cgcggttaac gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca
    12001 aagagatgat gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt
    12061 cgccattaaa ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga
    12121 caacttgcgt taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg
    12181 agtggccgga aacctcatag tgaccgccca ccagttggcc tgcatcgctt tgtagcgtac
    12241 gcgcggcatt ggcaataaga ttcagatact cagactcttc cggggccttc gccagcataa
    12301 aagaggagga tgctcgcgta tgcagcaact gctccagtgc gaattgcagt cgcggttgag
    12361 tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat
    12421 agctatcggt atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg
    12481 ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga
    12541 aagcaccgac aatactcctg gcatgggcgt taaagctcac aggatggaga ttcttttctt
    12601 cactggccta aaaagctgat attctgtaaa gagttacacg gtaacattga gatcgctatg
    12661 aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag
    12721 catcgcgaag gggagttaat cacccgttac atagaagcca gtgccgccca ggaagccgtc
    12781 gatgtgttgc tctcgctcga aaatgaaccc gtgctggtaa atggctggat tgacaagcat
    12841 atgaatccgg aactggtcaa tcgcatgaag caaaccattc gggcaagacg aaagcgccat
    12901 tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg atctggaatt tattgtctgg
    12961 caacgtctgg ctggtcttgc gcagcgtcgc ggtaaaacgc tttctgaaac gattgttcag
    13021 ctgattgaag atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc tctgaagcag
    13081 gatctgcagg cattgctggg taaggaataa ctgacgaaag tcagttcaat ttactaaagg
    13141 caaaaaaaac cccgcagcag cggggttttt ctaccagacg agaacttaag cttgcggctg
    13201 agttacaacg tctttgatac ctttaacttc gatctctacg cgacgatccg gagccaggca
    13261 gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga
    13321 ttcgcccata ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc
    13381 aacaacagac tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg
    13441 gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga tccaggttgc tcagctggct
    13501 gtacagctga tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa
    13561 cagaacgtca gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc
    13621 cggagcaact actggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag
    13681 catgccgttg tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt tggtccactg
    13741 gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc cagcgaagac
    13801 cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt ctgcacgcca
    13861 taccatacca cccagacgag tgtagatgtc caggtcgtca gtgattgggt aacccagttt
    13921 agcggtcagt tgaacgccct gagctttgta tgcaccgttt tcaacgctgc ctttgtacgg
    13981 catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa cctggtaacc
    14041 accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt tgatgaaacc
    14101 agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt tatctttcgg
    14161 agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga tagctgtctt
    14221 tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc aacgagataa
    14281 cacggttaaa tccttcaccg ggggatctgc tcaatattaa ctctaccgat atcttcggct
    14341 tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga
    14401 actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct tttttgtcag
    14461 cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt
    14521 agtcacgact gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt
    14581 acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt ggcgtgagag
    14641 gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca
    14701 agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa
    14761 ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaagggga gagctggcta
    14821 atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt
    14881 tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc
    14941 aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc
    15001 ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac
    15061 gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt
    15121 atggatgcac agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg
    15181 caaattttgt cgttggtgac gggaaaacat aaattaatct tgccccttaa gaataagttg
    15241 cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa
    15301 aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc
    15361 aacgttgggc acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgacga
    15421 cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc
    15481 acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt
    15541 taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg
    15601 tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga
    15661 aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg
    15721 gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg
    15781 gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg
    15841 cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc
    15901 tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca
    15961 atctcaggca gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg
    16021 tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg
    16081 cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca
    16141 agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata
    16201 tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg
    16261 gtaacgttcg gttcgctgga catatttgat accaccgacg ccagttcagc atcacggttg
    16321 tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc
    16381 tccagtatgg catcgagata ccggcagttg gcctctgtgg cgcgttcgag cattcgcggc
    16441 agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa
    16501 ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagcaagtta
    16561 aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gggcatattg cacgttacgg
    16621 aaggcaaaaa agagcacgcc ggtaataacc agtagcacca gctgcccttc cagtgacggt
    16681 acaaaccaca gcacaggaat gccaatggcg ataccgacca gcgtaccaat aatccttaac
    16741 ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag
    16801 atccaatacc cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa gcacaacacc
    16861 agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga
    16921 cgcagccaga tatcactcaa tccgtgcggg ctatcatcag cgagctcatt ttcgtcatta
    16981 ttatggggca gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg
    17041 cgtaaattgt tcagcaaaaa tcccagtgtt ttaagtaaat cggcaggcgc gccgttatcg
    17101 cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt
    17161 tgataaggct gacgcagcaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc
    17221 gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt
    17281 tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa
    17341 tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag
    17401 agtttcgtct gattcaatgt cgccatcagc tgaccgttgg cgagagccaa atcgtacagc
    17461 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
    17521 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
    17581 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc
    17641 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
    17701 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
    17761 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
    17821 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
    17881 cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt
    17941 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
    18001 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
    18061 gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgcc
    18121 gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag
    18181 ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg
    18241 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
    18301 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
    18361 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag
    18421 gtaaaaatca gcataatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
    18481 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
    18541 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
    18601 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
    18661 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
    18721 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
    18781 tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt
    18841 ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
    18901 tcactggcag cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca
    18961 actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg agcaaacctc
    19021 tggcgttcag caacaaatcc gccaggctat gtcggcgttg ccgttgccgg ttaaccgact
    19081 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat
    19141 tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc
    19201 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt
    19261 taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct
    19321 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
    19381 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg aacggctaca
    19441 taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg
    19501 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact
    19561 ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
    19621 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga
    19681 aaaattacag cgtcgtcttg catcacgcct cgatcgttgg gtaagtctga tgcggatgca
    19741 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
    19801 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
    19861 aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggtcg
    19921 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
    19981 ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg
    20041 tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt
    20101 ccatgaaaac tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag
    20161 tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagt tgaaaaagcc
    20221 gctaaacagc ttaaccaatg gagacgaaaa agccgtcacg ttattggatg agagtcaact
    20281 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttcttatcct
    20341 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
    20401 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat
    20461 catcgttggc ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat
    20521 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
    20581 gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc
    20641 ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtagcgagaa aaccgtaaga
    20701 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
    20761 taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat tatgaagtct
    20821 gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg caacaaggct
    20881 ttcacgtcag gatcgtgcgg cacggcattt agtgggtccc agaagaaaat caatacatca
    20941 attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac
    21001 atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt gcctgttgca
    21061 tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt
    21121 ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc
    21181 agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct
    21241 gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt
    21301 tttccgccca ttgtaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact
    21361 tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac
    21421 ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt
    21481 tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg
    21541 taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa
    21601 ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa
    21661 aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca
    21721 gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg
    21781 gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc
    21841 cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata
    21901 ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc
    21961 aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat
    22021 agacatattt cccgcttttg ctctcaatca ttcgtattag ctgcatggtt ggcatgtcgg
    22081 ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag
    22141 gtattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc
    22201 ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgc
    22261 caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg gcagacgttc
    22321 cggaaaaagt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcgc
    22381 aggaagctat cgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc
    22441 aagcggcagt actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta
    22501 ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg
    22561 gggatttgtt cagggattag ttacgcagac gcggggcctg gagttgtttg cggatggtct
    22621 gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct
    22681 gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc accacgtgat
    22741 accacggagc ggcgcgaagc tcgtcattca ccgccagttc atcaggcgat ggttcagaga
    22801 gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga
    22861 cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttaccc
    22921 ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca
    22981 gatatagccc ttccgggtag gtagcgatca ccggatgatc ggctgcctga cggaactgct
    23041 ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata
    23101 aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgtct tcattcagca
    23161 actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc aactggcttt
    23221 tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc
    23281 gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca
    23341 gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc
    23401 tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga
    23461 aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct
    23521 ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt
    23581 gttcttcaat cggcagcagg gcaggcggca actcgccggt gacggggcct tgggtcagct
    23641 ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca cattccgggt
    23701 acagcgtttg cagggcacta attaatgccg cgcgctgata ctctgcccca gcactcagca
    23761 gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt
    23821 ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt
    23881 tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa
    23941 acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt
    24001 ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg
    24061 caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg
    24121 ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat
    24181 tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt
    24241 cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca
    24301 ccacacagta cgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg
    24361 gcagcgtgga agtggttgct tgcggtgaag aagggcaggt agaaaaatta gtgcagtggt
    24421 taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc
    24481 cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc
    24541 cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat aaatagcggc
    24601 tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg
    24661 gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttctg
    24721 gcgacagcga aatcccttcg ttctttgcaa tcaccactgc cagcggttca ctccactggc
    24781 tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca
    24841 taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa
    24901 agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag cccagaatgc
    24961 tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat
    25021 agttcgtttc accgccatga atgatgttgc tggtttcaaa caaaatagcg ccagaggaga
    25081 tcagaatgaa gaccgcgctg atcgccagat gcagagcagg cagttgcagg aagatattag
    25141 caaccatacc aatcagtacc accacaatac ccgccatcag cataccgccg aggaacgaca
    25201 tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc
    25261 ccagtgccat agcgattacg tcacccattc cggcagacag ataggtgttc agaattggtc
    25321 cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat
    25381 tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac
    25441 ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa aaggccagcg
    25501 tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac
    25561 ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta
    25621 attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat
    25681 ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga
    25741 gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta tcttatctgg
    25801 cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccgaag
    25861 aaaaacaaat aaaaatcagt gtaatacgtg aaattgctga aaagttaatc aaatttaaca
    25921 ccaagaaaaa tcaaggtatt aggaagttgc tcatagctag tataccaagt ctttttgcca
    25981 gaaatttttc tggattgtct acttgctaac cacgagcggc aaatccagtg ttggtgtgat
    26041 ttttgtttta cgatcgtaaa tcaacacctg attttctgtt ttatgcccgc tgaaaatttg
    26101 tttgtcgcga ctgctgcctt cgtaatctga gatccccttg gcttttatgt cgtgaaaatt
    26161 gcacccgaac ggaacgccaa ctttttgctc ggctgcgcgt ttagcctgat tccaccagtt
    26221 attcagcgtt ttagctatga ctttcccgcc tttggttgta ttgatcacat attcgcatgt
    26281 acaggaagat acatttcggg ctaactggat cgctgtacgt aatcgcggtg accattcctt
    26341 gatttgtttg gtgccggttt tgttttgctc aatgtagatc cctttatcca taatatcctg
    26401 ccatttcaac tcaagcacat caccgagtct tgccgcacag agataggaaa tctccattgc
    26461 aatgcgtaac tgtggaattg cttccgcata tatcgccgca tactgttcat cggtgatgta
    26521 aacagtgcgg gctttaagag agaattttct gactcctttg catgggttat tcttcacata
    26581 cccacgctca tatccccacc cgtatactcg actcaggctt gccagttcat gatttgcctg
    26641 ggtcttgctc tcaagccctc gtttatccat gaaaattctt acctgctcag ttttgacatt
    26701 atcagcaagc acttttccga ataccatcag caacgccttc tgatgttgtc gataatcttt
    26761 ttgggttcgg ggggccagtt ctgtaaatgc aggggagtcc ataaacatgt gccacaattt
    26821 agctacggtc attatgttgt ggagttttgc tttttccagt tcataatttt gccagacttt
    26881 agctacgctg gttttccgca ctcttcctag ccctatactt cttgtacttc cttcgggttt
    26941 ccacacgtaa ctgtaaccat tcgatctaac ccgcggtggc agtacattat cttttttatt
    27001 ttttcttggt cttcccattg ttcagcgcct caaaatcggg ttcagcagaa accagttcag
    27061 atgtttttgg catcgttgtc agaccgtgtg gaatatccct gcggagaact atgggttcgt
    27121 ttttagggcc gattacaaat gggatgccgt gcagtcttaa ctggtgttgc tgttttgtgt
    27181 atcgctcgta tttcgtgatc tctttaatct ctgctggaga tagagttaat tcgtacatgt
    27241 ggtcacgtgc ctttacagca tgaccgccgc cactatagct ggtgggcggc gatatgggtt
    27301 gaacatcaaa aatcagcctg actcgggagc agtttttgcc agatggctga aacgtatttc
    27361 gcctggtaac gtgcatcgtt cagagcgtta tggcgttcac cttcgaatgg aatagtcgtt
    27421 cttgcgtcga aatccatcac cagtcccaga gcaaccatcg ttcttacatc gcgatcattg
    27481 gtgtaacgcc atgggcaggg gatcccctgc cgttcatatg aacggcgtaa aatcacgttg
    27541 tcgaaagttg caccgttacc ccagacctga acaaaaaatt caccggagtt ttcgtcgata
    27601 aattcccgta attgcagcag tgcatcatct aacgggattt catcggtaag aatggcggat
    27661 tgcgcttcgc gtgactgttt cagccaccac ttaatggtgt cccgatcaat gactccgcca
    27721 gcagtttcca gatcgatagt tttgctgaat tctggcccca tctctccggt ttgcggatcg
    27781 aaaaatattg cacctattga gataatcggg gcatcaggat tttttcccat tgtttcaagg
    27841 tcaatcatca gatggtgcca caacctgctg gtggatgaga tttcatgatg accgttcact
    27901 ttaattaagg gatttgctgt ttcgccagtt ttattatcgc tggcgtgata ctgatcgctg
    27961 ccagtgttct ccttgtgcag atgttcagtg ccttccattt cctccggatc atttttctga
    28021 gcttcagcct gattctcttc atcgaatgtt tccaggtagg ttgcgttccc catcaccgca
    28081 ccacagtcag gacagttgcc gccaccggtc tgaccgcatg cggtgcaaac tttttccagt
    28141 tcctgttgca ctgctggttc tggttgttgt tcttctggcc cgttttgttg cgtatccggg
    28201 ctgttttgtt ccgcttctgg ctcattttgt ttcacgttgg gctgattctg gtcctcagcg
    28261 tcgcgactct ggatcccttt cacccatttc ggatcattcg ggtcgctaat cccttcaaca
    28321 aattcaccac gtgatgcagc aagcaattta tcggcgtcag gctggctgat attggctgcc
    28381 tgcataattt tgtttacttc gtcagcggta acttttaccg gctctgattg ttcggaatct
    28441 tcagcggtat ccacattttg cggtaagccc gtgtatgtgc cattttttcg ggcaaaatat
    28501 tcttcttttg agatttcggt accgccggca gccagcgcct tgtccagacc agaaagtttg
    28561 tttgccctgc cgtatttttc cccgccctta tcggtaaaga cgaaataaaa tggtccttca
    28621 cgctctacag atggttcagc ttccaccagg atttcatttt tttgagtatc ggattctgcc
    28681 gtctccactg gagcagtttg tgctgctgac ggctggagag catcagtaga gctctggtct
    28741 gtttcttcat gttcaaacac gccctttgtc gtcaggtatt cactgatata tttgttcagt
    28801 gcaacgggat ctttgtgaat atcgatcgga cgttcgcgaa caaggccaaa aatagtctga
    28861 cggtcatagc caagtgcttc gggctgtttg cgcattgatg ctgagatacg cttccagtct
    28921 tcgcgatcct tgtcgataac ctcatttgtc gcccagcgat ggatggtacc gtcaatgttt
    28981 cctgcatcca catcaccggg ccagagggcg caggccagtt cattgtcgag tgttttccat
    29041 gtctgtttgt attcgcggcg aatggcaaca gtgacagccc cggttttttc agcagcgttt
    29101 tcagtgttct gttggttgac tctggcgcgg gtgagatcaa caacagacgt gtattttccg
    29161 gtttccttgc gttcaccttc gcgacgtttt ttccagatgc gcatctctgc ctgaatttca
    29221 ggccatttag cgccaggctc acatttatgc ttaacccacc cgatggcatg cagtttaagt
    29281 tcaggataca tggcgttaac ttctggcatt ttcatcaacg cttcaacgat atgtccgccg
    29341 aatgttgcca tgtcttcctg tagtaattcc tgtgcgctaa tcaccatatc aacggtgatg
    29401 ttttcgcatg tgtcgaattt aaccaggact gcgttctgta cttcagggga cagcttgtca
    29461 aaattgacgt tcatcggatc ggattctggt tcgaccggta caaaggaagc tgactcctca
    29521 tcccagcggt tttcctgcat atattcggta tcccaggaat cgagggcagg gcggggtatg
    29581 ccgggtttat cctcgcagac aataaattta taagcgcagt cctgagctgc cgggaattgc
    29641 tccagaaatt gccagtgaaa ttttgcgcgt gcgcgacgct catcaccggc ttcaatggca
    29701 gtggccaccg caacagcgct atcttctttt atggcctgtt cgtcaggaat agcagcgcaa
    29761 ataaagattt tactcatt
//
LOCUS       DAZGCS010000062        29647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGCS010000062 DAZGCS010000000
VERSION     DAZGCS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.062"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(129..776)
                     /gene="gloC"
                     /locus_tag="ACHK01_002835"
     CDS             complement(129..776)
                     /gene="gloC"
                     /locus_tag="ACHK01_002835"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO8097764.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(803..1351)
                     /locus_tag="ACHK01_002836"
     CDS             complement(803..1351)
                     /locus_tag="ACHK01_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO8097765.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1532..3379)
                     /gene="ldtD"
                     /locus_tag="ACHK01_002837"
     CDS             complement(1532..3379)
                     /gene="ldtD"
                     /locus_tag="ACHK01_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8097766.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3640..8100)
                     /gene="mukB"
                     /locus_tag="ACHK01_002838"
     CDS             complement(3640..8100)
                     /gene="mukB"
                     /locus_tag="ACHK01_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO8097767.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRAPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8100..8804)
                     /gene="mukE"
                     /locus_tag="ACHK01_002839"
     CDS             complement(8100..8804)
                     /gene="mukE"
                     /locus_tag="ACHK01_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO8097768.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDENEENQPDSGEEE"
     gene            complement(8785..10107)
                     /gene="mukF"
                     /locus_tag="ACHK01_002840"
     CDS             complement(8785..10107)
                     /gene="mukF"
                     /locus_tag="ACHK01_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO8097769.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10104..10889)
                     /gene="cmoM"
                     /locus_tag="ACHK01_002841"
     CDS             complement(10104..10889)
                     /gene="cmoM"
                     /locus_tag="ACHK01_002841"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO8097770.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11025..11804
                     /gene="elyC"
                     /locus_tag="ACHK01_002842"
     CDS             11025..11804
                     /gene="elyC"
                     /locus_tag="ACHK01_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO8097771.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11781..12674)
                     /locus_tag="ACHK01_002843"
     CDS             complement(11781..12674)
                     /locus_tag="ACHK01_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO8097772.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12828..13574)
                     /gene="kdsB"
                     /locus_tag="ACHK01_002844"
     CDS             complement(12828..13574)
                     /gene="kdsB"
                     /locus_tag="ACHK01_002844"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO8097773.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAAPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13571..13753)
                     /gene="ycaR"
                     /locus_tag="ACHK01_002845"
     CDS             complement(13571..13753)
                     /gene="ycaR"
                     /locus_tag="ACHK01_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO8097774.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13805..15037)
                     /gene="ycaQ"
                     /locus_tag="ACHK01_002846"
                     /pseudo
     CDS             complement(13805..15037)
                     /gene="ycaQ"
                     /locus_tag="ACHK01_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            complement(15074..16060)
                     /gene="lpxK"
                     /locus_tag="ACHK01_002847"
     CDS             complement(15074..16060)
                     /gene="lpxK"
                     /locus_tag="ACHK01_002847"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO8097775.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYSGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16057..17805)
                     /gene="msbA"
                     /locus_tag="ACHK01_002848"
     CDS             complement(16057..17805)
                     /gene="msbA"
                     /locus_tag="ACHK01_002848"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO8097776.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFDHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17842..20106)
                     /locus_tag="ACHK01_002849"
     CDS             complement(17842..20106)
                     /locus_tag="ACHK01_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO8097777.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTIFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITTTDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAIHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGVRLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGRICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGMNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20313..20597)
                     /gene="ihfB"
                     /locus_tag="ACHK01_002850"
     CDS             complement(20313..20597)
                     /gene="ihfB"
                     /locus_tag="ACHK01_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO8097778.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20757..22430)
                     /gene="rpsA"
                     /locus_tag="ACHK01_002851"
     CDS             complement(20757..22430)
                     /gene="rpsA"
                     /locus_tag="ACHK01_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO8097779.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22541..23224)
                     /gene="cmk"
                     /locus_tag="ACHK01_002852"
     CDS             complement(22541..23224)
                     /gene="cmk"
                     /locus_tag="ACHK01_002852"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO8097780.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23397..24161)
                     /gene="ycaL"
                     /locus_tag="ACHK01_002853"
     CDS             complement(23397..24161)
                     /gene="ycaL"
                     /locus_tag="ACHK01_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO8097781.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24330..25613)
                     /gene="aroA"
                     /locus_tag="ACHK01_002854"
     CDS             complement(24330..25613)
                     /gene="aroA"
                     /locus_tag="ACHK01_002854"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO8097782.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25684..26772)
                     /gene="serC"
                     /locus_tag="ACHK01_002855"
     CDS             complement(25684..26772)
                     /gene="serC"
                     /locus_tag="ACHK01_002855"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO8097783.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26971..27663)
                     /locus_tag="ACHK01_002856"
     CDS             complement(26971..27663)
                     /locus_tag="ACHK01_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO8097784.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27793..29553
                     /gene="ycaO"
                     /locus_tag="ACHK01_002857"
     CDS             27793..29553
                     /gene="ycaO"
                     /locus_tag="ACHK01_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO8097785.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7196 a         8057 c         7206 g         7188 t
ORIGIN      
        1 cccctgcaat attttgaatt tgcacgattt tgtaggccgg ataaggcgct cgtgccgcat
       61 ccggcataaa caaagcgcac tttgccagaa atccaggccc gcattaaaag cgggcttttt
      121 tatgtgcctt accagacggg catttcgtct tgcaggaagg gattatgcag gcgttcataa
      181 ccaagtgtgg ataatggtcc gtgacccgga ataaatgtaa cgtcatcccc cagtggcagc
      241 aatttatctt tgattgaaga aatcagttga ttatgatcgc cacgcgggaa gtcactgcgc
      301 cctactccgc ctttgaaaat aacatcgcca gaaatcagca gctttgcccg atcatcaaaa
      361 aacacgacat gacccggcgt atgcccaggg caatgtaaca cctgtaaagt cacattccct
      421 atgctgatgg tatcgccttc gttcagccaa cgatctggcg tcagcggctg gcactcttcc
      481 agaccaaaca tacgactttg cgcaggcaag ccttgcagcc agaactcatc ttctttttcc
      541 gggccaaaaa ccggcacgcc gtaatgttgc gccagttccg ccgctgcgcc aacgtggtcc
      601 agatgaccat gcgtcagcag gatctgcatc agtgtcaggc cgctgtcatc aacttcctgt
      661 ttgatttttt ccgcatcgcc gccaggatcg accagtgcgg ccagacgggt ttgttcacac
      721 cagattaatg aacagttctg ggagaatgcg gtgaccggaa taatacgata gttcatactg
      781 cccctgtttc gttaagcaat tactaccagt gccgtgctgg cccggtatca atatgcacaa
      841 agttactacg tggataatat cctacaccac ctgcgcgcat agataacgcg gctttgcgaa
      901 tattgcttaa cgcgatacct tcaatatgga aatccatcgc ctggccttta gtgtgatagc
      961 ttttcttcgc tactccacgg ctgcgggcgc gtagttcatt gttggtatca atagaacgat
     1021 aaccggaaat gagttgcacc ggtttgcgcg tgcctaacaa cccttgcagg cgatacaact
     1081 ggtcgaataa tcctgggtcg atggacttta ttttgttcgc gcggtaatcg cggaaaaaat
     1141 ggttaagttt tgccaattct tcctgaatat agcctctgcc atcgaaaaac tccgctttga
     1201 ttgactctcc ggtatgaaga ttattgagtg tcaaaatgcg cgggcgtggg gtagagagtg
     1261 ttgcaaacgc aggggtcggc aggatggcgg caccgagtgc aacgccacca agcgccagca
     1321 atttgcggcg attagcgtcg aatttgtcca tgataatcaa gtctacaggt caatgttatc
     1381 gtttatatgc acttctggcg cacaagaggc accttaaccg cccgtaaagc agacgtcaag
     1441 atgactcaac cccagcagtg acgggggctg cagagaatcg cccccaatca tgctaattat
     1501 tacgacaact gatttccccg aactacttca tttacctgat taattgttcc gctttcgata
     1561 cgatttgcga gctggatcgc gcaggcagat cataattgta aatatctgta cgatactggg
     1621 tacgaccatc tgcaccaaca aaggccgtca ggtagtagag attcaccgga atcgactgcc
     1681 gaatattgac gtaacgtgta tcaccctgct tcagcgcatc agaaatacgt ttgtcattcc
     1741 agcctgcatc ctgcaacagc atattcgcca gatcggaagc tttattcact cgtacacagc
     1801 ctgagctcaa tgcgcgtgta tcacgcttga acagattgtg gttcggcgtg tcatgcaaat
     1861 aaatggcctc tgaactcggc atattgaatt tatagcgccc cagcgagttc cgtgggcctg
     1921 gagcctgctg gaagcggaac ggtaaattcg aggccgtgat tgtagaccag tcaacctgcc
     1981 atgggtcaat cgcttctctg ctgttccagc cgcgcatcac cgtatagcca tggctttcga
     2041 gatatcccgg atcgttgcgc acttttggca gaatatcttt gcgtgccaga gttggaggta
     2101 cgttccacgg cgggtttacc actacgttgt taagggcact gctcatcatc ggcgttttgc
     2161 gatcggggcg accgacaatg actcgcgaat ccagcacctg attgccgttc tgatagtaga
     2221 ccagcgaata ggccggaatg ttaaccatga tcccggtaga aagctctgtt ggcagcaagc
     2281 gcaatcgctg gatgttgaga gccaacacgc cagcacgctg ggcgggcgtt acgtttaacc
     2341 agtcacgtgt tgccgggcca atagcaccat ctgcccccaa tccttgccat gcctgaaaac
     2401 gtttaacggc ttccaccagt tcattatcgt aggcggcgcg aacggcaggc gcaggtttac
     2461 tacgagatgt cgtttgcttg tccatcggct tagtttctgc tgtttcaaca gtaacagcgg
     2521 atgggctgac taccgcgtca gttggcgtgt catcgccagg tagagtaatt ttcggccccc
     2581 cgtccaacat gcctgtgcgt tgcaatattt cgcgcaacgc cggtacgtcg ttactccact
     2641 gccctgggcg caacgttgct ttgccggtca gttggggcca cggtttggtg tcactgagta
     2701 aggccagtaa cgattcatgc atcgccgcat attgcggatg ctgcggtgcc agtccagcaa
     2761 caaacgtagg caattgacct ttatccagcg ccagctgcca ttggttaatc accgagagcg
     2821 gcggcgttgc aagtgcataa ggtttactgc tatatagcca gcgagtgcct ttgaccggaa
     2881 tatttgcaat gaaatggaga tagcccatca tcgcatcaga gagcactacg tcgcgtgcca
     2941 tcccgttaac accaggatcg gtcagtaact ctacccattt attaaactgc ggctggaaac
     3001 cggcaatcgc cacctctgcc agctgttgct ggaaggcttt aacagcatca cggttttccc
     3061 acatgggttg catatcgcgt gcggcataca acagttgaag ctggttaaga taaaccggct
     3121 tgtaacctgc gggtaattga gattcgattt gcgtgcgggc tttttctgcc gcaccttcag
     3181 gcaatggctg gatccccgcc attattgccg tggcttgcgc ctgcggcagt gcctcgccct
     3241 gttcactgac agccaccggg ctgtcgccag ggattacttc aggctcatcg gcctgcgcat
     3301 tgaacagtgg agcgaatgtt acggccaggc acaaactgat tgccgacagc cgacgaccac
     3361 acatcatatt aagcaacatc ccttgccccc tgtttttatc atactctgaa tagccaggct
     3421 gttattttac gacttgcctg tacaacgcca atactcacga aagtatgacg agcaagcctg
     3481 ttactcatca ggcttatctt cagtatataa acgccgatac gtttttacat aacctaatgt
     3541 aaagaagttt aaagaaaagc gcgccgtaat gtgccacttt tttagtgcag aaacggagtt
     3601 ttcggaaaaa gaaaaggcgg cattgctgcc gccttaattt taactcgcct gagaaggcgc
     3661 ttcgtcactt cctggaagcg tttcagggag ttgcggcgca aatcctcgca ggccgacgac
     3721 gtgaacgtgt tcggtattct ggaagacttt acgcaccagt ttataggtgg tgcctttctc
     3781 cgggctgata ttttccggcg ctgcgatgat gagctgcatt tgcaaacgtt cacacaattc
     3841 aaacagcgtg gcgatagaac gagcatccag tcgcgctgct tcatcgagga acagcaggcg
     3901 gcaaggagag atatctttac cgcgcaggcg gcgagattca tcttcccagc tttgtaccac
     3961 catcaccaga atcgacatac cggtaccaat cgcctcaccg gtcgacaatg caccagactc
     4021 tgcgcgcaac cagccatcgg aaccacggtt aacctcaact tccatttcca gatagttgcg
     4081 gtaatccagc agttcttcgc caatggtctg cggcgcgcgc tgccccatat caatctgcgg
     4141 attaagacgc tgatacaatt tcgccagcgc ttccgagaag gtcagacggt tgctgttaaa
     4201 cagatcctga tgctgctcgt gctgttcaga gagcacatcc agcagcatgg cgtgcgtttc
     4261 acgcacgttc acgttcagac gtacgctgtt cacctgacca aacgatacgt tctgcaaccc
     4321 ctggttgagc atacggatac ggttctgctc gcgctgaatg gttttgcgaa tgatgttcgc
     4381 cacgctgcgg gaactgatcg ccagtttctg ttcgcgggag gttaactcct cggtcagtcg
     4441 gctaagttcg atttccatct gctcgatggc ttccaccgga tcatcggtac gaataatatc
     4501 ctgacgaata cgttcacgca gatgctgata aaccgccacg aagaactgaa ttttacgttc
     4561 cggacgtttt ggatcttccg acatgcgcag cacgtcgcgc agatgttcgt tatccgccac
     4621 cgccagacgc agcgcaccca atgccttatc cgacatagag cgcaaatcat cagcggagag
     4681 ataagccagc tcgcgacggt gtaagcgccg ttcaacgccg ttatctttca ccatgcgcat
     4741 cactgcacac cagcccgctt tggcggtcac tacctgctcg cgcatctcaa agtaatcccg
     4801 ctccagcttg cgcagtttgc gggtcagatt gtccatctcc gcttcgcaga aagtcagtgc
     4861 tttttccagc tggttgcggc gggaccggtt attactcagt tgcgcatgca gctcgtcacg
     4921 gcgaattcgc gcccgctctt ctgccccgct atcggcgcgc acgccgatat cctgcaattc
     4981 acgttgcaga tcgttgagta gctctttttt ggtgtcgtaa gaacttttca gcgaagccag
     5041 cacctggttg tactgactca actgcgctgc gtgaccacgc aacgcttcac gagcgcgagt
     5101 acgctccgct tccgcctgtt ccagacgttc acgcagcttt tcgttgagat cgctattacc
     5161 gctcagcatt tctgccgagt cagaatagct aaagtgcgca cgacgctgca ccacttccgt
     5221 cagggcaaac gcctgctgac gggcatcgcg ctgcatctgc tgagagtacg cgtaatcttc
     5281 ttttagctgt tcgaactgtt ccgggtcgct ctgcaatacc gaaacgatcg gttccagttt
     5341 cgccagttga ttgccaaact gctgaacaaa acgcgcggct tcctgggctt catccagacg
     5401 ttcgcggatt tcatcgacgc gatccgccag gctgtcatca gccaacaggt tgagacgcgg
     5461 cagaatgcgg ttcagcgccg taacgccttc tttcgcctgc tcaaactgaa tacgctgctg
     5521 ctggttatca ttttcatgat tactcaacgc ccgctccagt tcgacgcgac ggctgttcag
     5581 ttgacggatt tctgcttccg ggtcagactc aaacgcaacc gccagatgac tgccgataaa
     5641 gcggctgaac gcctgatgca gacgctgagt tttctgtaca tcaaaggaga gcgtggcgaa
     5701 gcgttcggaa agcacttcac gctcggcatg gaggctttca atacggcttt cacgcgcagc
     5761 acgaccaaac agcggcactt ccgggaaacg tgaataacgc cactgacgat cggcgatttt
     5821 aaccactacc gctttttcca gctcatcaac gctgaacacg ctgtcatcga atgactgcgg
     5881 atccccttcg atcaaataga gatcttccgg gcaatcggtc aagccttcca ggtgttcagt
     5941 tacctgtgac agatctggca ccacgatggc gtggcgtgac gggccataca gcgctgagaa
     6001 gtacggcgca tcttccaggc taacgtcgtc ataaatttct gacagcagca cgccgccaaa
     6061 acgctccgcc agcgcgttca gacgctgatc ttcagagccg ccaggctggc ttaaacgttc
     6121 gatctcttca tcgacagcgt ttttgcgcgc gcccacttca tcgcgttcaa caatcgcctc
     6181 tcgctcacgc tccagcaact gttgcagata ttcggtgacg tcctggctgg aggtaaactc
     6241 ttcaccgcac tgctcgctca actggttgag actgttttgc gctgccagcc aaaccggcgc
     6301 acgctgcatc aaactctgaa tgcgagactg cagctgttcc tgttcctggc gcagtgccat
     6361 gcgctcttca cgggcgttag acacgctatc ggaaagagag gcaatgcgcg cttccagctc
     6421 ctgatgcaga gcttccagtt catcaatatc aaaattcttg ccctgacgtt tgcagaagtc
     6481 tgccagcagg cgttcggctt cttgctgctc gcgcagacgc tgttccagtt cacttaagcg
     6541 catccgcaac ggttgaacct gctctgccag gtgacgctga tcgacccctt cgcgcagcag
     6601 ttcgcgggcg acatcccaag cctcgttacg cgccagcggg ccgttgattg ccaccaccag
     6661 ctgataagcc tgctcaaact ggctgtgcgc ggtttgcgcc atgctcattt tctgctcaag
     6721 agagagcatt ttttcagtcg cttccagctc tttcgcctgg aaggtttcca gccattcggc
     6781 ggcgctgtcg gcggttaagt ccggcagatg gcacagttct ttggcgcgat taagcgcagc
     6841 aatcgcctgg ttatactgga tcgcgcgcgt ttgctggacg tccagcgcct gctggtagtc
     6901 agcaagctgg cttttcagct cgtccacttc cagttcggca gcttccgcac gagcctcgtt
     6961 ctcttcctgg cgttcgatgg cttctgccac cacttcgttt tgctcttcca gacggatctg
     7021 cagctcatcg agatccgctt cgtagcgttc gatcttctcc tgctgacgca gtgcggtttg
     7081 caccaggttc aggtgatcgc tggccgcctg ataatccgct tccagatcgc cttcggcacc
     7141 gttgtgctct gccagctcac gcgccatatc gacgtgtttg tactgctcag ccgccagttg
     7201 ctgacgcgaa gtatgtagct cgcgacgaaa ctccagggct ttgtcgagat ggacacggcg
     7261 ctcgttggcg tgacgcatgt agtccgccgc cacgtagttg gtagcttcgc tgatcagatg
     7321 cttaaacagg tcacggtccg actgggtgac acgaatcgct tccagcgtca tgcggttttc
     7381 acgcagcgcc gcttccatgt cctggaacgc tttacgcacg ccgctgtttt ccggcaacag
     7441 gtagtcgcgc agagaacggg taatggcact ggagatcccg ccatacagcg aagcttcgat
     7501 cagacgatag aatttgctac ggtcagatgc ggagcgcaga cgacgcgcga tgatgcccag
     7561 atcgaacatc agcgagtggt aatcggtaat ggagttgaac tgtttaaact gcacgccttc
     7621 catcgcctcg agcttgtctt tcagctcgtt aagcggcagc acgcgcgcct ggcgttcgtt
     7681 cagggtttcg gtcaccagct gcgtcggcag caccgacatc ggcagtccct gaatggcaaa
     7741 tggtttgata tccactttac gatcgcgtcc ggcaacctgt tgcagacgca caccgaccac
     7801 cacgcgctgg tggcgcgagt taatggtgtc gagcatcgaa taacagacac ccgctttcag
     7861 cttaccgtgc agacctttat cgcgcgaacc gctggtggcc ccggcttccg tagtgttacg
     7921 gaaatgcagc agggtcaggt cggggatcag cgccgtaacg aacgccgcca tagtggtgga
     7981 tttacccgcc ccgttaccgc cagaaagcgt cgtgaccagc tcgtcaaggt caaaagttcg
     8041 ggcaaaaaag ccgttccagt taatcagcgt cagtgagcga aatttaccgc gttcaatcat
     8101 tattcttcct ctccgctatc tggctgattc tcttcgtttt catcgttgag ttgcagatga
     8161 ttttcaatcg gcattgcttc gccatcacga atcaggcgac gctgtgcttc acggggatcg
     8221 tcgccagcac gcacatcggc tccgaagcgg aacaccgatt cggtaatgcg gaacttgctg
     8281 ctgtcgtggc ccataaacca caccatgcct aaacgacgca gacggttgag cgaagaacgt
     8341 actttctcct gcaacttctg acggtcaacg tctgaaccgg ttgaacggtt gttcaccagt
     8401 ttcagcagtt tcgcttcatc ggccagggtg agcagttcgt cgtacagttc ctgctgggtg
     8461 aaaatcccct cattcgccag ccgttccgga ctgagataga gataacagag gattttcccg
     8521 accatcatat ccagttccga caagacggaa cgagggatca gcgtggtgga acgtgggcgt
     8581 aaatagaaga acccttctgg tgcgcgaata agctcaacgt tataacgcgc gtaaaactct
     8641 tccagatatt cctgaaaatc catcaggaat gcatgattat ccagttcgtc gaggccaata
     8701 tggcggcctg aacgtaaggc gctgtccagc gccggaaata acggattcgc cagcgcctgc
     8761 gccagcttaa ccggcatcac ttgttcaata tttgtcgatg acatgcgcct gtaccttggc
     8821 tccgtaatca ttaatcggct gccatttcgc tggcagcccg gtgaaatctg cttgcgctac
     8881 gccaagacgt accgcctgat caataacaat gcgcgcaacg tcaaagtgac gtgcacgcgg
     8941 atactgtgac agatattcgc gtaccaccag accaagatcc agcggcactt gtctggtttt
     9001 gtacacagca agttgttctt cgatgatcgc cgccagctgt tcgcggatct cgttaaactc
     9061 ttcgtattct aaatctggcg gaagttcccc agtcacttct tcatcgcgca gtgccatctc
     9121 ttcgtcacgc atatccagca gacgatcggc attggcataa gttagcgccc acggctcatc
     9181 aaaataggtt tgtaccgact gacgtaaacg ctgggcaaag acgcggtttt tatccatatc
     9241 gatcgcagta cgaataaatt tgtgtacgtg gcgatcgtag ccaatccaca agtcgatgga
     9301 ttgctggccc cagctgataa tacggtcgag tttgctctgc agatcgaaca ccagacgatc
     9361 gacaaaatgc aggtcgtcat gggtcatcgt cgcatcctga atgcgcaaca gattagcctg
     9421 caacttgtcg cctgccgctt ccagcgtatc ctgcaattca cgcagcgttc cggaagtttc
     9481 tgaaagcaac aattcacagc tggaaatcgc cgcccgccag tctttgttca gcaactgggc
     9541 aatatcgtcc ttcacctgct gctgctgttc gtccatcaga cgttgcgtca ggtcgatact
     9601 gtcgaaaatt tctgctaccg aatatttcag tggcgcatag acattacggt gccagtgaaa
     9661 ttcatcaccg ccctcttcgg cggcatcagc tgcgcgtttg agttcacccg ccacaatcga
     9721 caactgcata gaaagacgca gcgtagaaaa ctcgcgctga cggatgtagt agtcagtaat
     9781 gccgatgccg agcggcgtca gacggtaaat tgcgttccct tccgcctgct cgctggtaaa
     9841 gcggttcagc agacgttgac gcaccatgtc gttgatcgcg ttattggcgc gcacgccgat
     9901 ggtttcgctg gtttgctcaa acgcatcact cacatggcgg aatgcatcca ccagctcgcc
     9961 ttcactcatc tcaccatcca gacgctcgcc gttcagcgtg gcaaccgcca gcagaaaaga
    10021 gagtcggtct accggcagcg agatggagaa gtcatttttt ctggcccagg caaccagttc
    10081 ggggactgtc tgggaaaatt cactcatact ttatccttgc tctgcggttt gcgcgcggtg
    10141 acatgaatat aacgccccag ggtaatatac ggttcctgac ggcaataacg cgtttctaat
    10201 tcaagtaatg cttcatagca gtcgcgctgc tggtgcttct cgcgcagata atcatgaaac
    10261 acgcgaacgc ctgtcttacc cataatttgc caaccagctt cttccagcca cagataaacc
    10321 tgcgccgggt cgcgtggata atctggcgaa agcgtccgtt ttttcttttt cggcattccc
    10381 gcctgcacgt aatcaaaatt cccggcgacc atgttatgca tcaacaaacc atgcgcattg
    10441 tagaacatta acgacaacac gccgcctgga cgcaacactg accacagggt ctgcaataca
    10501 ctgcggggat cggccaccca ctcgagcacc gcatggaaca atatcagatc aacgggcgtt
    10561 tccaaatgcg aagcaacatc ctgagcggcg caatgtataa attgcatgtt gtcgctcaca
    10621 ccttttgctt ctgccgcctg ttttgcgcgg tcgatcatct gcgcagaaag atcgcataaa
    10681 atgacctgat gcccacgctc ggccattttg attgcggtct gcccttctcc accgccagca
    10741 tccagcacac gcagtttttg cgggcccatt tccgccagca cgcgatcgag atcctgccac
    10801 agaatagcct gtcgaagctg ccctttggtg gtgccgtaaa tgttacggga aaacttttcc
    10861 gcaatatcat caaaattgcg atcctgcatt gctttctcca ccggttggct aagacgtttt
    10921 ctgataaaga cgctattttg tcacagccac ggcgagaatg aacccgtctg gtgtaatatc
    10981 gctggcgatc ccttgctata tggttaaaaa aggaaccaga aaggatgctt tttacactga
    11041 aaaaagtgat tggcaatatg ttgctgccac ttccgctgat gttgttaatt attggcgctg
    11101 gcctggcgct gctgtggttt agccgttttc aaaaaaccgg gaagattttt attagcattg
    11161 ggtggctggc actcttgtta ttgagtttgc aaccggtagc cgaccgcctg ctgcgtccca
    11221 tcgaaagcac ctatccgaca tggaataatt cgcaaaaagt ggactatatc gtggtgctcg
    11281 gaggtggtta tacctggaac ccgcagtggg caccgagctc taatttaatc aataacagtc
    11341 tgccacgcct gaatgaaggt attcgcttat ggcgcgaaaa tccgggatca aaactgatct
    11401 tcacaggagg cgtagcaaaa accaatacgg tgagtacagc ggaagtaggt gccagagttg
    11461 cgcaatcgct gggcgtgccg cgcgagcaaa ttatcaccct ggatttgcca aaagataccg
    11521 aagaagaagc tgcggcagtg aaacaggcga ttggtgacgc ccctttcctg ctggtgacct
    11581 ccgcatcaca tctgccgcgg gcgatgatct tttttcagca ggaagggtta aatccgctac
    11641 cagccccggc gaatcagttg gccatcgact cgccgcttaa cccgtgggaa cgggcaatcc
    11701 cttctccggt ctggctgatg catagcgatc gtgttggcta tgaaacgctt ggccgcatct
    11761 ggcagtggct gaaaggtccg tcaggcgagc cacggcaaga gtgattttga tgccagatcg
    11821 aagttgcgcc gactaaatcg tccggtatta accagttgcg caacttcatc ccataacaca
    11881 tacaaccaac gccgccagat gaacgactcc gccactggcg cgcgttgcag gtaactccag
    11941 agcaaatctt ccgccagaga attatccatt agtcggaaca gttcgtattc tcgcggtgcc
    12001 caaagcatta gtcccggccc gaccatcgcc agtaactgat cgctgcgcga atctttcagc
    12061 atgctgcgta aacagaagtt accgtgaatc aacacacaat tgtcgttaaa gccttcaaat
    12121 aatgccggga gacattcgcg agtgcgaaac aggatccgct tatcctgcat cgtcagaccg
    12181 gtattattga actgattgag cgtggtccat agcacttcaa catgttgcag gtaccatgag
    12241 ggccagaaat tttcctgagt attgtcgacc gcgccgacgc aaccgcgact gtcctgacgg
    12301 tgccaggcca gtaaggcttc aacgatttgg tctttgagct gttcccagcg ttctggtgtt
    12361 cgggctggtg cctccaccga aacaccacgc atccgctcca gtagcaggac atcagggccg
    12421 gggtgttctt catgcgtcat cacaccataa atagtcggca tacggacagt cccacttctt
    12481 gccagcatgg tggttttcca tgccagttgt cgggcttttc cgggcgtact aaagcttctt
    12541 gccattaacg gcattgggtt tccctggctg tcatacaaag cccacaacgc cgtatccgct
    12601 ttttcattga cgcactcaat acgactgagt ttttcgccca gtaaatggct taattcggca
    12661 cgcagctgtt ccattcagat taccctcata aaagctactg ctttcataat gagcgccaat
    12721 ataaaagatg tcacctgaat gagatcaatt gttgaaaaga aaaattgagg aaaatcgtaa
    12781 cggtacgaca ctcctcccaa aattggctga agtgtcgtga agtgaaatta gcgcatttca
    12841 gcgcgaacgc gctcaagatc ttcaggggta tccacacctg tgccaggaac ttcctgagca
    12901 acagcaacat ggattttttc gccgtaccac agaacacgaa gctgctctaa catttcgatg
    12961 tgttctaacg ggcttggctg ccaggtgacg taacgacgga taaagcctgc acggtagcca
    13021 taaataccaa gatgacgcag gaagttatcg ccaacggttt caaggtcttt tgcaaaacgg
    13081 tcacgatccc acggaatggt ggcgcgtgag aagtacagag catacccttc agcgtcgaga
    13141 acgactttca ccgcattcgg gttaaacgct tcttccgcat tgtggattgg cgccgccaga
    13201 gtcgccatac ccacctgacg ctgagcgagg ttatcagcaa cctgacgaat gatggtcgca
    13261 gggatcatcg gttcatcacc ctgcacatta acgatcaccg tgtcgtcgct gaatgcgcat
    13321 ttttcgacaa cttccgccag acgttctgtt cctgactgat gatcggcgcg cgtcatacat
    13381 acttcaccgc cagcggcttc aacggcgcgg gcaacatcct catgatcggt tgccacgatg
    13441 atgcgctcgg cacctgattc acgcgcgcgt tcaagaacat gaacaatcat gggtttgccg
    13501 ttaatatcaa ccaatggttt accgggcaga cgcgtggacg cgtagcgcgc gggaataatg
    13561 accacaaaac tcatgattta ctctcatcag cagtcagcac gcgggcttcc gtttccagca
    13621 acactggaat gccatcgcgg agcgggaaag ccaggttatc cagtttgcaa ataagctctt
    13681 gtttttcttg gttataccaa agttttccgt tgcaaaccgg gcaggcaatg atttcaagca
    13741 aacgatgatc catagttcct ccagatggat cgggttatga atgcataaat cttatcataa
    13801 tcatttatgc gacggggtct atttcccagc cagtgcggca atcagtaaag agaccttgcg
    13861 ggcagcgtcc taatgtcacc cgcgttgcct gctgccagct cgcgaaatca gtaatcgcct
    13921 gacgtaaccc tttttgcagc attgtcgttg gtttaatgcc ttcctgtaac cacagagaga
    13981 taacttcaag gatgcctgtc tggcgatgca ttttggcatc cattcgccca actaattgcc
    14041 cacgatgtaa taatggcaga acaaaatagc catactggcg tttcggcgct ggggtatagc
    14101 actccagccg gtagctaaaa tcaaaaagct actctgcgcg tttgcgatcc cagacaacag
    14161 gatcaaaagg cgaaagtacc gcgctgtgcg ttgcagtgag ctttccggcc agcgcacgct
    14221 cgagtagcgg cagcaaatca gcatgcagcc aaagattgcc caatttttca acatgcacag
    14281 caatgatttg ctgctgttca gccctcgctt cgcgccacgc cgccagtgcc ggacgtttca
    14341 gccgatagta atctgccagc cactgttcgc ggaatattcc caggctacgc gcactgttat
    14401 ccagcatgat gatttctgct tctgtttgcg aaacgagatc gcgctcatca tcccagtcag
    14461 gcatgacacg gtgggttaaa tcataaacgc gctggaagtt gcgccgttca atcaccatca
    14521 cctttccggc agtaaataaa ccttccagat gccgtttatg cggcttccat tcccaccagc
    14581 cgcttgcacc tttacgagga tgctcaaaat cggctgaacg taccggcccc ttatcatgaa
    14641 tatgctgaat taactgtgca atttccgcct catgttcctg catccaggcg tctttgtatt
    14701 tccagcccat tttttcaggt gccagcatgc ggtggcgaat aagacgaaag tcgctacgcg
    14761 gcataaagca ggcttcatgc gcccagtatt ccattaattc gccacgcgcc agagactcat
    14821 ccagccactg ggcaggataa tttcccagac gactgaaaag caccagatat ggactacggg
    14881 caacaatatt gatggtatcg atttgcagca aggacatgcg ggagatcgtt gccggaatat
    14941 cctccaacga cgcttgacgg cggggtttgt ttaacaggcc ttgtgcggca aggtgaagat
    15001 tacgcgcatc agcaagggag aggtgcggca gcgacattca tgactccatc aatcgaacgc
    15061 tgccgcggcg taactagttg ccagaagcca gcgaggttag ttgcgtaagc agtttcgctg
    15121 gttcatcacc tgaaagctgt gcgtctacag gcaaatacca ccaattttct tctgcaaagg
    15181 cccggcattt caccgcatct ttttcagtca ttaccagcgt ttgcccggcg cttaccaacg
    15241 cactgacatc cgcatggttc aaagactgat gatcggccag cggtacacat ttttccggtt
    15301 gtacgccaca catcttcagc gtggcaaaaa agcgcggcgg atgcccaatc cccgccatcg
    15361 ccactacatg ttcaagctga gcaacgtcac aacgcgtacc ggtacgtaaa ttcaccgcct
    15421 gacccggcag cagatgcatg gggatttcac cgctgcgagg gacaccgccg ttgacgatta
    15481 ccgcatcaac cgactttaag cgccccgctc gctcacgcat tggccccgcc ggcaaccacc
    15541 agccattgcc aaagcgacgc acaccatcaa taacgacaat ttccacatca cgcgccagac
    15601 ggtaatgctg taaaccgtcg tcggttacga tgatctgcac atcagggtgt tgcgccagaa
    15661 tggcttttac cgcatcagaa cgaacgggag aaaccgcaac aggcgcatca gtgcgttgat
    15721 aaatcaacac aggttcatca cccgcctgtg ctgttgtggt atctgccgac aataacagcg
    15781 gataagattc agccttacca ctatatcccc gcgatacgac ccccacgcga ataccgcgct
    15841 gttgcaactg ttccaccagc cagacaacga ccggggtttt tccgttgccg cctgcggtga
    15901 gattaccaac cacgacaacc ggtacggggg cacgccaggc gcgcttcagt tttagtttat
    15961 agcaaagacg gatcgcgcca cttaccaggc catacaacca ggagagtggc agcaatagcc
    16021 gccacaaagg ggattcacca gaccagattt tttcgatcat tggccaaact gcattttgtg
    16081 aagttgcgcg taaacgccac ggtgctcaag taaatcgtta tgcgtaccgc gttccacaat
    16141 gacaccatcc tcgacgacca cgatttcgtc tgccttttca atggtagaca agcggtgggc
    16201 aatcaccaga gaggtacggt ttttctgcaa ctcatccagt gccgcctgaa tcgcacgttc
    16261 ggattcggta tccagagccg aggtagcttc gtccagaatc agaatcgggc tatcacgcaa
    16321 caaggctcga gcgatagcaa tacgctgacg ctgaccgcca gagagcagca cgccgttttc
    16381 accaatcact gtatcgagac cgttatccat cttattgatg aagtccatag cgtaggccat
    16441 acgcgccgct tcttcaattt gctcacggct gtactgttca gttcgtgcgt aagcaatgtt
    16501 gttagcaacc gtatcgttaa acagatggac attctgcgac accagagcaa cctggttacg
    16561 caacgacgcc agggtatact cgcgcagatc gtgaccatcc atcaggattt cgccttcatc
    16621 aatatcgtaa aaacgcgtga tcaggctggc gatggttgat ttacctgaac cagagcgtcc
    16681 aaccagagca accgtcttcc ctgccggaat tttcaggttg atgttacgca atgcaggtac
    16741 gtcacgtccc ggataagtaa aggtgacgtt gcggaattcc acgtcacctg tcgcacgctc
    16801 gatcacgcgc ttaccttcgt ctttctcctg ctcactgtcc agaatggtaa acagcgtctg
    16861 acaagccgcc ataccgcgct ggaactgggc gttaacgttg gtcagcgatt tcagcggacg
    16921 cataagtgca atcattgaag agaaaacaac ggtaatcgta ccggcagtca ggctatccat
    16981 gacacttggg aagcttgccg catacagaac aaacgccagc gccaaagagg cgatcagctg
    17041 aatgatcgga tcagagatgg aagaggctga aaccattttc atcccctgaa gacgcattcg
    17101 gttgctgact ttatcgaagc gtttcgtttc cacttcctga ccaccgaaaa tcaatacttc
    17161 tttgtggccc tttagcattt gttcggcgct ggtggtcacc tgccccatgg tgttctgcat
    17221 gtttttactg atgttgcgaa aacgcttcga tacaacgcga atcgcaatcg aaacaatcgg
    17281 tgccagcaca atcaaaatga tcgacagttg ccaactgtaa tagaacatca tgatgaacag
    17341 gccgatgatt gacgcacctt cacgcacaac cgttatcagt gcgccggaag aagaagaagc
    17401 aacctgttcg gaatcgtagg tgatacgtga caacagcgta cccgttgact gtttgtcaaa
    17461 aaaggaaacc ggcattccca tcatgtgatc aaacaggcgg cgacgcatgg tcattaccac
    17521 ctttcctgat acccaggaga tacagtagct ggagacatag ctggtgatac cacgtaaaat
    17581 catcagcccg atcaccacca gcggcatcca caccagcacg gagcgatctg ttttaccaaa
    17641 gccatcatca agaagtggct taaggagcga taacatgaag gtatcgctgg ctgcgttgag
    17701 gattaacgct acgcccgcca cgatcagacc cgctttaaaa ggcgcaatgg ttggccacag
    17761 tcggcggaac gtctgccacg tagagagatc tttgtcgtta tgcattcaaa aaaccagcat
    17821 ttgttgaaat agccgcatat tctacccgtt atccactggc acgccaaacc actgatgata
    17881 ccaacgaggt aaaatttgct ctctgagact gctaatccgc cagccttgcg ctgaaaaatt
    17941 gaccgttact tgcccctgat gaggagtatc gagccacgta taaccctgtt gttgatagcg
    18001 atgcttaact ttgttagagg gcagtcgcca tgcgttatag cgcgatgccg atgcgagtgc
    18061 cacttttcca ttcactcgct gaattaatgg caacgatgat gaggtattac tgccatggtg
    18121 aggtacctga agcaatgttg cctgcacttg ctgccagtaa cggcttagca tcttttgttc
    18181 agcggggact tcaatatcac cggttagaag aatgctattc atcccgtcat caaccttaac
    18241 cacacaggaa tggttatttc ctttatcgtt gctagcttgt aaaggccagt gcacgctgaa
    18301 acgcaatcct tgccattgcc acgcttcgcc acgcacacag ggctgatgat gttcccagtt
    18361 taacggactt ctgatccata acatcggcca tgtatgcaat attgagtcca gccctccccg
    18421 gtgatccaga tgttcatgac tcagaataac gccttccggt tcaagattat gccagtggag
    18481 ccaggggata atcagttgtt gcccactatc cccttcaggc caggccagtc ccgtgtcata
    18541 gagaatcgct ttgccgtttc tggctatcac cattgccagc ccttgcccga catcaagcat
    18601 gtacacttgc cactcgtcag gtcgaggttt ttgccacagc ggccagcaca tcagtaagcc
    18661 tccagccaca cacattgctg gcaacgttcg ccaggcgttt aatcgccata ccaccagtaa
    18721 gaaccatggg gaaaatgata gccattgcca acgttcagca atgttgatcc acccttccgg
    18781 caaactcttt aacccccaga aaagtaaagc caaagaccgg tcggcaagaa accataaccc
    18841 ttgctccagg attaacggcc cgcttaaatg cacaaccatc gcggcgagga tcaacggaac
    18901 cgtgataaat gtcaccaagg gaattgcaaa tagatttgca ataaacgagg tcagactaat
    18961 gccatgaaat atgacgattt gcacgggcat aagcaggagt gtgattccca gttgcaaatg
    19021 aatgagggaa acaactgcac gcaataccgg cggcagttgc cactcaggac agggaaacca
    19081 ctggtaccaa aaaatcagtg ccgcgaccgc agcggcagag agccataaac tttgcgagag
    19141 aatggcaaca ggatccatca gcaaaattgc cgccagacaa catatccata catcccagcc
    19201 actccactgt cgcccactta acttaagcat tccccacgta gcaagcgcca ccacggtacg
    19261 caatgcagga ggttgcattc ccgtcaacca ggcataaaaa gcagcacagc agattctgcc
    19321 aattaatggt atttgccagt ggatccagcg cccaggcaga aaaatttgtc cactgcgaat
    19381 gagtccggca gccagcaacg ccgcaaaagc gatgtgcaat cccgatatcg ccattaaatg
    19441 cgccgttcca gtatcacgca tgatattttt gatttcttta gggacggata accgtacccc
    19501 catacctaaa ccaagaataa ccgcattcca catatagggt tgcagcgttg tttgtagtga
    19561 cgccagatac tgtgcccgca ggctacaatt cggttcaatg acacttgcct gcagaaagcg
    19621 gccggtgagc ggctgatgct gggcaatggc ataacgctgg ctatcaaagc cgccatcatt
    19681 aagttgacca tgaatggcac gaactttgag tttcattgac cacagttgtc cagcacaaac
    19741 cgcttgcgga agatattcgc catacagcac gaggcctggc gcagggaata tacgtcgacc
    19801 ttgtagatga gtgatttgac cgtaatgagt ggtcatgccg tcagtagtag tgatctcaac
    19861 aattgcatcc tgcgtcgcac cagttaaggt ttctcctgcc cacaaaattt gctttgccgc
    19921 taatataccc caaacgaaaa acagcaacgt cagcgcgata taacgtacgg ttttaacagg
    19981 gataaatgcc agtacgcagg cgaagatagt cagaaacgca agggttaatg tcccaggcaa
    20041 ttggggcaaa atcagcaacg gaaaaattcc gcaaattatg catacgccga ctgtcgttat
    20101 tttcatgcac ccctccctgt ttatggaaag tgtgcaactt tgttgagcgg ttgtctggtg
    20161 ggaattttgc gttccccgag gcacgttcag aagaatttaa cgagagggtg aaaactgctc
    20221 aaaactctgc gaagcagctt ccagttccag attagagaaa tgaaaaaagc acccgacagg
    20281 tgcttttctc tcgttcaagt ttgagtaaaa acttaaccgt aaatattggc gcgatcgcgc
    20341 agttctttac caggtttaaa gtgaggaacg tattttcctt ccagttctac tttatcgcca
    20401 gtcttcggat tacgtccggt acgtggtgcg cggtagtgca aagagaaact gccgaaaccg
    20461 cggatttcaa tacgctcgcc ctgcgcaaga gtcgaggcca tatgctccag catctctttt
    20521 actgcatctt caaccgtctt ggcgggaatg tgcgattgct gggtggcaag tctttctatc
    20581 aattctgact tggtcatgat tcctccggtt ccttaaaggc aaattaagcg gctgctgttg
    20641 ctgcattgat taagggcggc cgtagccgcc cttagtgctt gattacagga cgaaacctgc
    20701 aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc ccgaagagtc agagaattac
    20761 tcgcctttag ctgctttgaa agcttcagcc attgcgttgt tggagaagtt tgcatcttcc
    20821 tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt cgtctttcgc acgaacagac
    20881 aggctgattg cgcggttttt acgatcaacg ccggtgaatt tagcttcaac ttcgtcgcca
    20941 acgctcagaa ccagggtagc gtcttcaacg cggtcacggg atgcttcaga agcacgcagg
    21001 taaccttcaa cgccgtcagc cagttctacg gttgcgcctt tagcgtcaac tgcagttact
    21061 ttaccggtta cgatagcgcc tttcttgttc agagcaaccc agttgttgaa cggatcttct
    21121 gcgagctgtt taacgcccag ggagatacgt tcacgttctg cgtcaacctg cagaacaact
    21181 gcagcgattt cgtcgccttt tttgtattca cgaactgctt cttcgcctgc aacgttccag
    21241 gagatgtcag acaggtgaac caggccgtcg atgccgccgt ccaggccgat gaagataccg
    21301 aagtcagtga tagacttgat tttaccttca acacggtcgc ccttgttgtg ggtttccgcg
    21361 aactgctgcc acgggttagc tttgcactgt ttcagaccca gggagatacg acgacgttct
    21421 tcgtcgatat ccagaaccat aacttccact acatcgccaa cgttaacaac tttggacggg
    21481 tggatgtttt tgttggtcca atccatttcg gaaacgtgta ccaggccttc aacgccttct
    21541 tcgatttcaa cgaagcagcc gtagtcggtc aggttggtca cgcgaccagt cagtttggta
    21601 ccttccggat aacgtttagc gatagctacc cacggatctt cgcccagctg tttcaggccc
    21661 agggatacac gggtacgttc gcggtcgaac ttcagcactt taacagtgat ttcgtcgccc
    21721 acgttgacga tttcgctcgg atgcttaacg cgtttccagg ccatgtcagt gatgtgcagc
    21781 aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt cagtgaggtt cttaacgata
    21841 cctttaactt ccatgccttc ctgcaggttt tccagcagct gatcgcgctc tgcgctgttt
    21901 tcggattcga taacggcacg acgagaaaca acaacgttgt tgcgcttctg atccagcttg
    21961 attactttga attcaagctc tttgccttcc aggtgcagag tgtcacgcac cggacgaacg
    22021 tctaccagag aacctggcag gaacgcacga ataccgttca gctcaacagt gaagccgccc
    22081 ttaactttgc cgttgataac accggtaaca gtttcagcat cttcgtaagc tttttccagc
    22141 gtgatccagg cttcgtgacg tttagctttc tcacgggaca gcagagtttc accgaagccg
    22201 tcttctactg cgtccagagc aacgtcaact tcgtcaccta cctggatttc cagctcgccc
    22261 tgggcgtttt tgaactgctc agccgggatg gcggactcag atttcagacc agcgtcaacc
    22321 agtactacgt ctttgtcgat agcaacaaca acgccacgaa cgatagaacc cgggcgggtt
    22381 tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag attcagtcat gtttaatctt
    22441 caggtttata tttaacgtcc acctggctcc atgccggatg gggttgttta acatacccgc
    22501 tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc ttatgcgaga gccaatttct
    22561 ggcgcgcgta ttgtagcgct ttttcaatca cttgctcaat gcttaaggtg gtggaatcca
    22621 acactaaagc atcggctgcc ggaaccagtg gcgctaccgc tcggttacga tcgcggtcgt
    22681 cgcgttcttt gatctcggcc aaaaggcgct caaagttaac actaaagccc ttctcctgca
    22741 actgtagcat gcggcgatgc gcacgttctt ccgaggaggc gtcaaggaaa attttcactg
    22801 gcgcatcagg gaataccacc gttcccatgt cgcggccatc ggcaatcagg cctggtaatt
    22861 cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac gcgtgggaat gccgcgactt
    22921 gtgaagctgc attcgccact tcctgagtac gaatttcgcc gctgacatct tccccttcga
    22981 ggatcacttc cagattgcca ttggttgaca caaaacgtac atccaggtgg gatgccagcg
    23041 gtaccagcgc atcttccgat gcaacatcaa catggtgatg taatgccgct agtgccagta
    23101 cgcgataaat tgcacccgaa tccagcagat gccattgcaa tgcttccgcc atagccttac
    23161 acaaggtgcc tttccctgca ccgcttgggc catcaatggt aataaccggg gcaattgccg
    23221 tcatctttat ctccttaaaa caggcgtacc gttaacataa ccgcgcgcat tatacgcgcc
    23281 gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt tgtctgtatt cagatgggga
    23341 tttattgcgc aattatagcg ggctggcaga agaccagccc gaaagatgac aatgatttac
    23401 ttaccagagg cgatacgatc acggatgtgt tgcgcacgct ctgttgatgg tgggtgagag
    23461 tcaaacatgg actgggtgcg accgccatcc agactagcca gtttttcaaa gctgccaacc
    23521 agcccctggg tgctgatgcc acgtttcttc aacagatcaa aggagaaatc atctgcttcg
    23581 gactctttat cacgggagta tttagcattg atagcgcctt ctgcgatatc acccaattgt
    23641 gagcgggaaa gctgggaagc cacaccgctg gtagctgaaa tggcatcgcg tgcggcaacg
    23701 atcgcataag aagctttcat ttcagccagc gagtgcccca acgcgacgtg gcccagttca
    23761 tggcccagaa caccttcaat ttcgttgtca ttcatcatgt ccatcaggcc actgtagaca
    23821 cgaacacagc cgttcgccat cgcccatgcg ttgacgtcgc tggtcatata aaccttatag
    23881 ttgaccggcg tgccattaat attgttaccc aatgctttgg cgatttttgc cagacgttta
    23941 ccgtacttgc tggatttact tgcgacttga ttgccgctgt ccatttcggc acagccctga
    24001 ttggcaatcg cttttgcatc ggcatcgctt aatgttgctg ctttataggc atttaaaccg
    24061 gagctgatag ccatattggt atcaataccg tgggtatttt gacaccctgt cagtaatgct
    24121 gcagaggtcg caatcgccag cagtaattta gtattcttca tgtgaatcat ccgttgatta
    24181 atcatatata aatattatgt atcaacaact tacattgaca agcgacgcta ccattaatca
    24241 aaatgactca caaggtccga aaaaaaacgc cgacaatgtg ccgacgtttt gataaagaaa
    24301 gatttggcta tttattgccc gttgttcatt caggctgcct ggctaatccg cgccaactgc
    24361 tcgaaataat ccggaaatgt tttggccgta catttgggat caagaatcgt cactggtgta
    24421 tctgacaacg ccaccagcga gaaacacatc gccatccggt gatcattgta tgtcgcgatc
    24481 tcggcaaagt tcagtttttc cggtggagtg atacgaatgt aatcgtgccc ctcttctact
    24541 tccgcaccga ctttacgcag ttctgttgcc atcgcaaaca ggcgatcggt ttctttaaca
    24601 cgccagttat agatattgcg cagcgtggtg gtgccttttg caaataacgc cgccgtggca
    24661 atggtcatcg ccgcatcggg aatatggttc atatccatat caatagcgtt cagttcacca
    24721 cgcgtgcagg aaatataatc atcgccccag caaatggtcg cgcccatttt ttccagcaca
    24781 tcagcaaagc gaatatcacc ctgcatactg ttacgtccaa taccggtcac ttttacagtg
    24841 ccgcctttga ttgctgctgc tgccagaaag taagaagccg aagatgcatc gccttcgacc
    24901 aaataagtac ccggagactg ataagactgc ccgcctttta cgacaaattg ttgatagtgc
    24961 tgattttcaa tttcaacacc aaacgtcttc atcagattga gtgtgatgtc gatataaggt
    25021 ttagaaacca gatcgccttt aatacgaatc accgtatctt ccggcgcaag aggcgcagtc
    25081 attaacagtg cggtgaggaa ttggctggaa acggagccat caacgtcaac gttgccgcca
    25141 gtaaagccgc cctgtaaacg caacggcgga taattttctt gttccaggta agtgatcttc
    25201 gccccgccca ggcgcagcgc atccaccaga tgaccaatcg ggcgttcttt catacgcggc
    25261 tcaccggtca gcacaatatc attgctaccc agacaaagag ctgccgccag cggacgcatt
    25321 gccgttccgg cgttaccgag gaacaactcc agggcacctt ctgcgtgtaa tggaccgccg
    25381 ttaccgataa tttcgcaacg cgtacgatcg gctgaaagcg tatagcttac ccctaacgct
    25441 gttaatgcat tcagcatatg gcgcacgtca tcgctatcca gcagattggt taatactgtt
    25501 ttgccgtgtg ctaatgccgc cagcaataaa gcgcggttag aaacgctctt ggaaccgggc
    25561 agattaatag tgccatcgac acgagcgatg ggttgtaacg tcagggattc catgaactca
    25621 actctctaca acagaaataa aaaccccaca ggctggctgt ggggattaag caaaatttcg
    25681 gcattaaccg tgacggcgtt cgaactcaac catgaagtct gtcagcgctt taacgccttc
    25741 cagcggcatg gcgttataaa tagaagcgcg cattccgccg accacacggt gacctttcag
    25801 tgcatgaagg ccagcagcaa aagactcttc aaggaacaat ttgtcaagcg cactgtccgc
    25861 caactggaac ggcacgttca tccgcgaacg gttagctttc gccacgtcat tgcggtagaa
    25921 atcgctgtta tcaatcaccc catatagcag ttctgctttt tgctgattga ttttatccat
    25981 ttcagctaca ccgccgttcg ctttcagcca tttaaagacc agaccagata gataccaggc
    26041 aaatgtcggc ggcgtgttaa acatggagcc gttatcgttg aggatggaat aatccagaat
    26101 cgacggacac gcgatattcg ctttgcccag caaatcttca cgaacgatga cgattgtcag
    26161 gccagccggg ccgatatttt tctgcgcgcc agcgtaaatc acgccataac ggctgacgtc
    26221 aatcggacgg gaaagaatgg ttgaagagaa gtcggcggcg accaccacat ctttgccgaa
    26281 gtctggcgtt tcgtcgatgg cgataccgtc gatggtttca ttcgggcagt aatgcatata
    26341 agcagcatta tcagagagtt gccattcacg cattggctta accgcgcgca gaccatcaac
    26401 agtcactttg gcgtcaaaga cattaggcgt gcagtatttt ttcgcttctt taatggcact
    26461 tgccgcccag taaccggcat caacataatc tgcggtggtt ttatcaccga gaatattcag
    26521 cggtaccgca gcaaactgac cgcgaccacc gccatggcag aataatacct tgtagttgga
    26581 ggggacatta agaagatcgc gaaaatcctt ctcggcttcc tctgcaacct gaatgaactc
    26641 tttgccacgg tgactcactt ccatcaccga cgtaccaaga ccgttccagt cgcgcagttc
    26701 ctgttgagcc tgtttaagca cctctgccgg tagcattgcc ggaccagaac taaaattgaa
    26761 gatttgagcc atttcccctc accacgttgc gttgctatta acccgcggtc aatcgattgc
    26821 gaccacaggt aatacattat tcctgtggct atcggtttta tcattcagtg acacacatcg
    26881 caatgagtaa aaccgttgag ggcaaaaatg tggcttgcat cacacaaaag aatctctcgg
    26941 attattagca aaaagccgat gtttttactg tcaggtcacc cgttttgccc gacttgcctt
    27001 cgtccattct ggatttgcac agcgcggaca taattgtttt tcccccgctt tcatatgaat
    27061 gatttcacta caacgaatac aggcatagtc cgccgactcc ggcaccactt tgtaacgctg
    27121 cgtacaatca ctgggtaaaa taagtaaacc cggtttcacc ttgtcatctt caaagaaata
    27181 gacactgatt tgcccgttgg tttcgagaat cgccagacgt acctgcccca gctgctccac
    27241 gccacgcaat cgtagctcca taaagaactc aaattccgtc atgttggagt tattgagttt
    27301 cgaccaggcc agctcgccat cttcaataat aacaaccggc ttaccttcca gtagatcttc
    27361 cagtttttca ctgtgcgcca tcaaccacat taccaggcgg tataacaacg ccagagtgat
    27421 aaagacgata agtaccggca ccatcggcac atcatcataa aacgccacat ctcccgccgc
    27481 tgaacccagc gtgagaatga ttaacacttc aaacagcgac atctgtcgca caccgcgtct
    27541 tccggtcatt ttgaggaaca aaaaaaccag tacaaaggta taaagactac gtagtgcaac
    27601 ttcgccaagg aaatcaaaag gcactttatc aaatgccata cggtggagat cgaacgcctt
    27661 catttttatg ctcttatcgt ggtttatccg ctaaaagcat agtcaacagt tcatcggcat
    27721 ggcggcggtg atagcgcctt tgagataaaa cccgtattat tgcgcgcttt ccgtacgact
    27781 aaagtgattt tcatgacgca aacatttatc cccggcaaag atgccgctct ggaagattcc
    27841 atcgctcgct tccagcaaaa actttcagac ctcggctttc agattgaaga ggcctcctgg
    27901 ctgaatcctg tgcctaacgt ctggtctgta catattcgcg acaaagagtg cgcactgtgt
    27961 tttaccaacg gaaaaggcgc aaccaaaaaa gcggcgctgg cttctgcact tggtgaatat
    28021 ttcgagcgtc tctcaaccaa ctactttttt gctgatttct ggctgggcga aaccatcgcc
    28081 aacggtccgt tcgtgcatta tcccaacgaa aaatggttcc cactgaccga aaatgacgat
    28141 gtgccagaag gactactcga tgaccgtctg cgcgcgtttt atgatccgga gaatgaactg
    28201 accggcagca tgctgattga cctacaatcc ggtaacgaag atcgtggtat ttgcggcctg
    28261 ccgtttacgc gtcagtccga caatcagacc gtttatattc cgatgaatat cattggtaac
    28321 ctgtacgtct ccaacggtat gtccgcaggt aatacccgca acgaagcacg cgttcaggga
    28381 ttgtctgaag ttttcgaacg ctacgtgaaa aaccgcatta ttgctgaaag catcagcctg
    28441 ccagagatcc cggcagacgt gctggcgcgt tacccagcag tagttgaagc catcgaaacg
    28501 ctggaagcgg agggtttccc aatcttcgct tatgacggtt cgcttggcgg ccagtatccg
    28561 gtgatttgcg tggtactgtt caatcctgct aacggtacct gctttgcctc tttcggtgcg
    28621 catcctgatt ttggcgtagc actggaacgt accgtgaccg agctgctgca aggtcgtggc
    28681 ctgaaagatt tggatgtgtt tactccgcca accttcgatg atgaagaagt cgctgaacat
    28741 accaacctcg aaacgcactt tatcgattcc agcggtttaa tctcctggga cctgttcaag
    28801 caggatgccg attatccgtt tgtggactgg aatttctccg gcaccacgga agaagagttc
    28861 gccacgctga tggctatctt caaaaaggaa gataaagaag tttatattgc cgattacgag
    28921 catctgggtg tctacgcttg ccgcattatc gtgcctggca tgtccgatat ttatccggct
    28981 gaagatctgt ggctcgcgaa taacagtatg ggcagccatt tacgtgaaac gattctttcg
    29041 ctaccaggca gcgagtggga aaaagaagat tacctgaacc tcatcgagca actggatgaa
    29101 gaaggttttg atgactttac ccgcgtgcgt gagctgttgg gtctggcgac cgggtcggat
    29161 aacggttggt acaccctgcg tatcggtgaa ttaaaagcca tgctggcgct ggctggtggc
    29221 gatctggaac aggctctggt ctggaccgaa tggacgatgg agtttaactc atcagtattt
    29281 agtccggaac gcgccaacta ttatcgctgc ctgcaaacgt tgttattact ggcacaggaa
    29341 gaagatcgcc agccgctgca atatctgaat gcgtttgttc gcatgtacgg cgcagatgcc
    29401 gtagaagccg ccagtgcggc aatgagcggc gaagcggcgt tttacggcct gcaaccggta
    29461 gatagcgatc tgcacgcgtt tgctgcacat cagtcgttgt tgaaggccta cgaaaagctg
    29521 cagcgcgcca aagcagcatt ctgggcaaaa taaaatcaaa tagcctacgc aatgtaggct
    29581 taatgattag tctgagttat attacggggc gtttttttaa tgccccgctt tacatatatt
    29641 tgcatta
//
LOCUS       DAZGCS010000063        29552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGCS010000063 DAZGCS010000000
VERSION     DAZGCS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.063"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHK01_002858"
     CDS             complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHK01_002858"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO8097786.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHK01_002859"
     CDS             complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHK01_002859"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO8097787.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3129..3428)
                     /gene="ysaB"
                     /locus_tag="ACHK01_002860"
     CDS             complement(3129..3428)
                     /gene="ysaB"
                     /locus_tag="ACHK01_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO8097788.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3603..4598
                     /gene="wecH"
                     /locus_tag="ACHK01_002861"
     CDS             3603..4598
                     /gene="wecH"
                     /locus_tag="ACHK01_002861"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO8097789.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4640..5077)
                     /gene="yiaA"
                     /locus_tag="ACHK01_002862"
     CDS             complement(4640..5077)
                     /gene="yiaA"
                     /locus_tag="ACHK01_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO8097790.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5123..5464)
                     /gene="yiaB"
                     /locus_tag="ACHK01_002863"
     CDS             complement(5123..5464)
                     /gene="yiaB"
                     /locus_tag="ACHK01_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO8097791.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5633..7087)
                     /gene="xylB"
                     /locus_tag="ACHK01_002864"
     CDS             complement(5633..7087)
                     /gene="xylB"
                     /locus_tag="ACHK01_002864"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO8097792.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7159..8481)
                     /gene="xylA"
                     /locus_tag="ACHK01_002865"
     CDS             complement(7159..8481)
                     /gene="xylA"
                     /locus_tag="ACHK01_002865"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO8097793.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8847..9839
                     /gene="xylF"
                     /locus_tag="ACHK01_002866"
     CDS             8847..9839
                     /gene="xylF"
                     /locus_tag="ACHK01_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8097794.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9917..11458
                     /gene="xylG"
                     /locus_tag="ACHK01_002867"
     CDS             9917..11458
                     /gene="xylG"
                     /locus_tag="ACHK01_002867"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO8097795.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11436..12617
                     /gene="xylH"
                     /locus_tag="ACHK01_002868"
     CDS             11436..12617
                     /gene="xylH"
                     /locus_tag="ACHK01_002868"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO8097796.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12695..13873
                     /gene="xylR"
                     /locus_tag="ACHK01_002869"
     CDS             12695..13873
                     /gene="xylR"
                     /locus_tag="ACHK01_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO8097797.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEIML
                     "
     gene            complement(13981..14805)
                     /gene="bax"
                     /locus_tag="ACHK01_002870"
     CDS             complement(13981..14805)
                     /gene="bax"
                     /locus_tag="ACHK01_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO8097798.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15125..17155
                     /gene="malS"
                     /locus_tag="ACHK01_002871"
     CDS             15125..17155
                     /gene="malS"
                     /locus_tag="ACHK01_002871"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8097799.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17333..18586
                     /gene="avtA"
                     /locus_tag="ACHK01_002872"
     CDS             17333..18586
                     /gene="avtA"
                     /locus_tag="ACHK01_002872"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO8097800.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18738..19211)
                     /gene="ysaA"
                     /locus_tag="ACHK01_002873"
     CDS             complement(18738..19211)
                     /gene="ysaA"
                     /locus_tag="ACHK01_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8097801.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19313..20161)
                     /gene="yiaJ"
                     /locus_tag="ACHK01_002874"
     CDS             complement(19313..20161)
                     /gene="yiaJ"
                     /locus_tag="ACHK01_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO8097802.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20362..21360
                     /gene="yiaK"
                     /locus_tag="ACHK01_002875"
     CDS             20362..21360
                     /gene="yiaK"
                     /locus_tag="ACHK01_002875"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO8097803.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21372..21839
                     /gene="yiaL"
                     /locus_tag="ACHK01_002876"
     CDS             21372..21839
                     /gene="yiaL"
                     /locus_tag="ACHK01_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO8097804.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21957..22430
                     /gene="yiaM"
                     /locus_tag="ACHK01_002877"
     CDS             21957..22430
                     /gene="yiaM"
                     /locus_tag="ACHK01_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO8097805.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22433..23710
                     /gene="yiaN"
                     /locus_tag="ACHK01_002878"
     CDS             22433..23710
                     /gene="yiaN"
                     /locus_tag="ACHK01_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO8097806.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23723..24709
                     /gene="yiaO"
                     /locus_tag="ACHK01_002879"
     CDS             23723..24709
                     /gene="yiaO"
                     /locus_tag="ACHK01_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO8097807.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24713..26209
                     /gene="lyxK"
                     /locus_tag="ACHK01_002880"
     CDS             24713..26209
                     /gene="lyxK"
                     /locus_tag="ACHK01_002880"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO8097808.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGGVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26206..26868
                     /gene="ulaD"
                     /locus_tag="ACHK01_002881"
     CDS             26206..26868
                     /gene="ulaD"
                     /locus_tag="ACHK01_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8097809.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            26861..27721
                     /gene="sgbU"
                     /locus_tag="ACHK01_002882"
     CDS             26861..27721
                     /gene="sgbU"
                     /locus_tag="ACHK01_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO8097810.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEMMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEDCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27715..28410
                     /gene="araD"
                     /locus_tag="ACHK01_002883"
     CDS             27715..28410
                     /gene="araD"
                     /locus_tag="ACHK01_002883"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8097811.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28442..28543)
                     /locus_tag="ACHK01_002884"
                     /pseudo
     CDS             complement(28442..28543)
                     /locus_tag="ACHK01_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28547..28810
                     /locus_tag="ACHK01_002885"
                     /pseudo
     CDS             28547..28810
                     /locus_tag="ACHK01_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28757..29497)
                     /gene="yiaT"
                     /locus_tag="ACHK01_002886"
     CDS             complement(28757..29497)
                     /gene="yiaT"
                     /locus_tag="ACHK01_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO8097812.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQVDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         7278 a         7604 c         7422 g         7248 t
ORIGIN      
        1 aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc aacagcgaaa
       61 tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga cggttgagac
      121 gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca accggctcac
      181 gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag tacggctcca
      241 gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc tctttcaggg
      301 tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccaaaatg ttggagacac
      361 gcttgttcgc cgccgccagt gcagcagctg cttcaagggt acggaagtgc gataccgctt
      421 tcatgcgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc gcctggatgg
      481 tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga
      541 taacatcatc aaccacgttg gcgttagtca gcttatcgcc atacagacgc actgcttctt
      601 cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc agcacgccaa
      661 gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg ccgataccga
      721 agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct accgggttag
      781 acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc accgcgacat
      841 cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg gtgtcggtga
      901 actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca cgggttgcgt
      961 ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg atgcggtcag
     1021 ttttgtcgcg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg cgcggcaggt
     1081 tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc gccagacgtg
     1141 gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat tcgatgttgg
     1201 caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg aagtatttct
     1261 ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat ttctcttcga
     1321 attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc agcaggcttt
     1381 cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg gctttaatct
     1441 tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga atttccggat
     1501 actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca
     1561 cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc agcagcaggg
     1621 tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc atcagtttcg
     1681 ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct tcggtgcttt
     1741 cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc agacgctcag
     1801 cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg ctcggtttgc
     1861 cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca cgatccggtt
     1921 gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca aaccattgaa
     1981 cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca gcaaaggact
     2041 cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc accagaaaag
     2101 ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag ccgagggctt
     2161 cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt
     2221 agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg aagctgtggg
     2281 cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc gccagcagct
     2341 gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca tccgcgtatt
     2401 cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg taggtggttt
     2461 tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca
     2521 tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac tccagaccac
     2581 caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac acttcccagc
     2641 ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt
     2701 gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc tgaatattgt
     2761 ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg ttggggtttt
     2821 cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc gccatcggtt
     2881 ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg acttccatgt
     2941 ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag gtcaggatca
     3001 agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga tacgtgtgga
     3061 tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga aacgggaaaa
     3121 agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca
     3181 aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa ctgttcgaag
     3241 gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg atccttgcat
     3301 aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc tttctgcacg
     3361 ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa gaaagcgttc
     3421 atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat cggtatagtg
     3481 gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa ctcagattat
     3541 tccagtgcgc caactgaatg atttatcggt cagacttttt ttaaccagat acagagacac
     3601 agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt ttaatggtgg
     3661 tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc cccgtcacct
     3721 gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta tttttcatga
     3781 tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg
     3841 gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg tttacctcca
     3901 tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt
     3961 ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg aagaacgtcg
     4021 gcggaaaaat gttgctggtg ctaatggtgg tgattggcat tatcgctaac ccaaacacag
     4081 tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc aatggcgata
     4141 ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg gacacacagc
     4201 ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt attatctctc
     4261 gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg tatctttact
     4321 gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa aacacgctgg
     4381 atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt atatacggat
     4441 tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa aattggccaa
     4501 tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta ctttctatgc
     4561 tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt tcacattgga
     4621 ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt gtttcttttg
     4681 gcgggtttat tccggtatca cgaatattct tctgcaccgc tactgcacca aacaagctta
     4741 agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca ttccacagac
     4801 ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata taataaatgg
     4861 aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac gcagcagaaa
     4921 acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc atctctgcat
     4981 tccatagccc taacagatag gtaacgatac caccaacgag agctatccat gacacaatac
     5041 taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt tcattcctga
     5101 atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga ccaaacaagc
     5161 agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt gcatttaaaa
     5221 caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata tgggtaaaaa
     5281 aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca aaagttgctg
     5341 tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag atccatagcc
     5401 caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt tttgaggttt
     5461 tcataacgat ctccatatct accagcgata cattacgagt aaccaacgaa agacaaaact
     5521 gaaaaatgcc attaacaaat gattttcaga ataaattcat actaaatatt aattaattag
     5581 tgagatatat agatgtgaat tatcccccac ccggtcaggc aggggataac gtttacgcca
     5641 ttaatggcag aagttgctga tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg
     5701 cataacgctg cgcatctggt agatgcgact gttctaacgg tagctgcggc aacaactcaa
     5761 tgagcgattt ctctggattc gccgcaatct gcgccagcct tgctgcgccc agtgctggcc
     5821 ccacatcccc ccccgtacgg taatcgagct gctgaccgct gatatccgcc agcatctgac
     5881 gccagtactc actacgcgcc ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc
     5941 aggcatgcac gacatccatg ccatctgcca gcgcataacc cacgccttcc agcactgctc
     6001 gcgccagttc attggggcca tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg
     6061 gattattgtg tggtgtacgc tcgccggaaa gataaggcag aaaccaaact ggctcggcac
     6121 tttcatcagc ctgttgagct gcagcgatta aagctgggac attgcacagg ccggttaatt
     6181 tcgcgaccca atccagacac gatgctgcac tcagcattac agacattaaa tgccaacgct
     6241 gcggtagcgc atggcaaaag ctgtgtacag cgctttctgg cttgcttaag aacccttcgc
     6301 tgacagcaaa atagaccccc gacgtcccca gcgataacat tgcctgatta gcatcaacca
     6361 ttcccacacc aactgcacca gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg
     6421 ccatacccca cgctttcgca acttcaggta acaaagcacc agtaatttcg ctgccttcat
     6481 ataatgcggg catctggtca cgagataagt cgcaagcctg cagcatgacg tcactccagt
     6541 cacgctttgc gacatccagc cacatggtgc cagctgcgtc agacatatcg ctggcaaact
     6601 cccccgtcat acgcagacgc aagtaatctt tcggtaataa tactttgtcg atttgacgga
     6661 atatctccgg ctcatgccgc tgaacccata gcaatttagg cgcagtaaat ccgggcatca
     6721 tcaggttgcc ggtaatcact cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt
     6781 gcgcacagcg cccgtcgttc cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca
     6841 gtaaggttgc tccatgcatc tggccggcaa tacccaatgc tttaacgtcc tgcagagaat
     6901 gctgatcgcc cagagctttc attgcgcgat cagttgcctg ccaccactgt tccgggtctt
     6961 gttccgacca gagtggatgc gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca
     7021 cctcaccctg ctcgttgagc aaaataactt ttacgcccga ggtgccaaga tctatcccga
     7081 tatacatatc gatcgttcct taaaaaaatg cccggtatcg ctaccgataa ccgggccaac
     7141 ggactgcaca gttagccgtt atttgtcgaa cagataatga tttaccagat tttccagttg
     7201 ctcctggcga ccactctgat gcaccggaga caaattatgt tcctgagcat atttggctaa
     7261 atctgccagt gacatttggc ctttcaggat ttgctggccc aattcgctat tccagccgga
     7321 ataacgctgc gcgatgcgtt tatccagctc gccatcttca atcatgcgcg ctgcaatttt
     7381 cagcgccagt gccatcgtat ccatcgcgcc gatatgaccg taaaacagat catatttatc
     7441 agtactttga cgacgtactt tggcatcgaa gttcagacca ccggtggtga aaccgcctgc
     7501 tttgagaatt tcatacatca ccagcgcatt ctcttccaca ctgttcggga actggtcggt
     7561 gtcccagccc agttgcgcat cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc
     7621 aatggcggtg gcaatttcat gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc
     7681 aatgttcagt ttaatctctt tttccagacc aaactgtttc aggaagccat agaccgtcgc
     7741 ggcatcgtaa tcatattgat gtttggtcgg ttcttgcggt ttcggttcga taagcaacgt
     7801 gccctggaaa ccgattttat gtttatgctc aaccaccatc tgcataaagc ggcccagctg
     7861 ttcacgctcc tgacgcaagt cggtatttaa cagcgtttcg taaccttcac gaccgcccca
     7921 caggacatag ttttcaccgc ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt
     7981 tgccgcccaa ctgaagactt caggatctgg gttcgtcgcc gcacccgcgc catagcgagg
     8041 gtttgtaaag cagttagcgg ttccccacag cagcttcacg ccgctctctt cttgcttgcc
     8101 tgccaggaca tcaaccattt gcgcaaaatt attgatgtac tcttttaacg acgcgccctc
     8161 aggggaaaca tccacatcgt ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc
     8221 aaatgcgaca tctgctttac gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg
     8281 acgattaaac gcccccacac caaacatgtc cgccccgttc cagcagaatg tgtgccagta
     8341 gcaggcggca aaacgcaagt gctcttccat acgcttgccc aacaccagtt cgtcgggatt
     8401 gtagtgacgg aatgctaacg ggtttgagga ttttgagcct tcataacgaa cgcgatcgag
     8461 ctggtcaaaa taggcttgca tattgaactc cataatcagg taatgccgcg ggtgatggat
     8521 gatgtcgtaa tattgggcac tccctttcag ttgctcaatt atgttatttc acactgctat
     8581 tgagataatt cacaagtgtg cgctcgctcg caaaataaaa tggaatgatg aaactgggta
     8641 attcctcgaa gagaaaaatg caataagtac aattgcgcaa caaaagtaag atctcagtca
     8701 taaatcaaga aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc
     8761 ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga ttgttactta ttaaagctgt
     8821 cctctaacta cagaaggccc tacaccatga aaataaagaa cattctactc accctttgca
     8881 cctcacttct gcttaccaac gttgcggcac acgccaaaga agtcaaaata ggtatggcga
     8941 ttgatgatct ccgtcttgaa cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag
     9001 aatctctcgg cgcgaaagta tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt
     9061 cgcagattga aaacatgata aaccggggtg tcgatgttct tgtcattatt ccgtataacg
     9121 gtcaggtatt aagtaacgtt gtaaaagaag ccaaacaaga aggtattaaa gtattagctt
     9181 acgatcgtat gattaacgat gcggatatcg atttttatat ttctttcgat aacgaaaaag
     9241 tcggtgaact gcaggcaaaa gccctggtcg atattgttcc gcaaggtaat tacttcctga
     9301 tgggcggctc gccggtagat aacaacgcca agctgttccg cgccggacaa atgaaagtgt
     9361 taaaacctta cgttgattcc ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct
     9421 ggttaccgga aaacgcattg aaaattatgg aaaacgcgct aaccgccaat aataacaaaa
     9481 ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg
     9541 cgcaaggttt atcagggaaa gtagcaattt ccggccagga tgcggatctc gcaggtatta
     9601 aacgtattgc tgccggtacg caaactatga cggtgtataa acctattacg ttgttggcaa
     9661 atactgccgc agaaattgcc gttgagttgg gcaatggtca ggaaccaaaa gcagatacca
     9721 cactgaataa tggcctgaaa gatgtgccct cccgcctcct gacaccgatc gatgtgaata
     9781 aaaacaacat caaagacacg gtagttaaag acggattcca caaagagagc gagctgtaag
     9841 cgttacgccc cggcgcggag cgggggcgtg atttctctcc atgccgcgtg aatgaattgg
     9901 cttaggtgga gtcgttatgc cttatctact tgaaatgaag aatattacca aaaccttcgg
     9961 cagtgtgaag gcgattgata acgtctgctt gcggttgaat gctggcgaaa tcgtctcact
    10021 ttgtggggaa aatgggtctg gtaaatcaac actgatgaaa gtgctgtgtg gtatttatcc
    10081 ccatggctcc tacgaaggcg aaattatttt tgcgggagaa gagattcagg cgagtcacat
    10141 ccgcgatacc gaacgcaaag gtatcgccat cattcaccag gaattggccc tggtgaaaga
    10201 attgaccgtg ctggaaaata tcttcctggg taacgaaata acccacaatg gcattatgga
    10261 ttatgacctg atgacgctac gctgtcagaa gctgctcgca caggtcagtt tatccatttc
    10321 acctgatacc cgcgttggcg atttagggct tgggcaacaa caactggttg aaattgccaa
    10381 ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa ccgacagcct cattaactga
    10441 gcaggaaacg tcggttttac tggatattat tcgcgatcta caacagcacg gtatcgcctg
    10501 tatttatatt tcgcacaaac tcaacgaagt caaagcgatt tccgatacga tttgcgttat
    10561 tcgcgacgga cagcacattg gtacgcgtga tgctgccgga atgagtgaag acgatattat
    10621 caccatgatg gtcgggcgag agttaaccgc gctttaccct aatgaaccac ataccaccgg
    10681 agatgaaata ttacgtattg aacatctgac ggcatggcat ccggtcaatc gtcatattaa
    10741 acgagttaat gatgtctcgt tttccctgaa acgtggcgaa atactgggta ttgccggact
    10801 cgttggtgcc ggacgtaccg agaccattca gtgcctgttt ggcgtgtggc ccggacaatg
    10861 ggaaggaaaa atttatattg atggcaaaca ggtagatatt cgtaactgtc agcaagccat
    10921 cgcccagggg attgcgatgg tacccgaaga cagaaagcgc gacggcatcg ttccggtaat
    10981 ggcggttggt aaaaatatta ccctcgccgc actcaataaa tttaccggtg gcattagcca
    11041 gcttgatgac gcggcagagc aaaaatgtat tctggaatca attcagcaac tcaaagttaa
    11101 aacgtcgtcc cccgaccttg ctattggacg tttgagcggc ggcaatcagc aaaaagcgat
    11161 cctcgctcgc tgtctgttac ttaacccgcg catcctcatt cttgatgaac ctaccagggg
    11221 tatcgatatt ggcgcgaaat acgagatcta caaattaatt aaccaactcg tccagcaggg
    11281 tattgccgtt attgtcatct cttccgaatt acctgaagtg ctcggcctta gcgatcgggt
    11341 actggtgatg catgaaggga aactaaaagc caacctgata aatcataacc tgactcagga
    11401 gcaggtgatg gaagccgcat tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag
    11461 tgaaattggc cgtaccgaca tccggtggct tttccgggct gaaatcactg aatttgcagg
    11521 tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt ctttacctgg accaccgatg
    11581 gtgcctactt aagcgcccgt aacgtctcca acctgttacg ccagaccgcg attaccggca
    11641 tcctcgcggt aggaatggtg ttcgtcataa tttctgctga aatcgacctt tccgtcggct
    11701 caatgatggg gctattaggt ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt
    11761 tgccacttac catcattgtg acgttggttc tgggactgct tctcggtgcc tggaacggat
    11821 ggtgggtcgc gtaccgcaaa gtcccttcat ttattgtcac cctcgcgggc atgttggcat
    11881 ttcgcggcat actcattggc atcaccaacg gcacgactgt ttcccccacc agcgccgcga
    11941 tgtcacaaat tgggcaaagc tatctacctg ccagcactgg cttcatcatt ggcgcgcttg
    12001 gcttaatggc ttttgttggt tggcaatggc gcggaagaat gcgccgtcag gctttgggtt
    12061 tgcagtctcc ggcctctacc gcagtagtcg gtcgccaggc tttaaccgct atcatcgtat
    12121 taggcgcaat ctggctgttg aatgattacc gtggcgttcc cactcctgtt ctgctgctga
    12181 cgttgctgtt actcggcgga atgtttatgg caacgcggac ggcatttgga cgacgcattt
    12241 atgccatcgg cggcaatctg gaagcagcac gtctctccgg gattaacgtt gaacgcacca
    12301 aacttgccgt gttcgcgatt aacggattaa tggtagccat cgccggatta atccttagtt
    12361 ctcgacttgg cgctggttca ccttctgcgg gaaatatcgc cgaactggac gcaattgcag
    12421 catgcgtgat tggcggcacc agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa
    12481 tgggggcatt tatcatggct tcactggata acggcatgag tatgatggat gtaccgacct
    12541 tctggcagta tatcgttaaa ggtgcgattc tgttgctggc agtatggatg gactccgcaa
    12601 ccaaacgccg ttcttgattt tgataaaaat tttctcaaag ccggttacgt attaccggtt
    12661 ttgagttttt gcatgattca gcaggaaaag aaccatgttt actaaacgtc accgcatcac
    12721 attactgttc aatgccaata aagcctatga ccggcaggta gtagaaggcg taggggaata
    12781 tttacaggca tcacaatcag aatgggatat tttcattgaa gaagatttcc gcgcccgcat
    12841 tgataaaatc aaggactggt taggagatgg cgtcattgcc gacttcgacg acaaacagat
    12901 cgagcaagcg ctggctgatg tcgacgtccc cattgttggg gttggcggct cgtatcacct
    12961 tgcagaaagt tacccacccg ttcattacat tgccaccgat aactatgcgc tggttgaaag
    13021 cgcatttttg catttaaaag agaaaggcgt taaccgcttt gctttttatg gtcttccgga
    13081 atcaagcggc aaacgttggg ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga
    13141 agaaaagtat cgcggagtgg tttatcaggg gttagaaacc gcgccagaga actggcaaca
    13201 tgcgcaaaat cggctggcag actggctaca aacgctgcca ccgcaaaccg ggattattgc
    13261 cgttactgac gcccgggcgc ggcatattct gcaagtatgt gaacatctac atattcccgt
    13321 accggaaaaa ttatgcgtga ttggcatcga taacgaagaa ctgacccgct atctgtcgcg
    13381 tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact
    13441 gttgcatcga ttattagata aagaagaaat gccgctacag cgaattttgg tcccaccagt
    13501 tcgcgtcatt gaacggcgct caacagatta tcgctcgctg accgatcccg ccgttattca
    13561 ggccatgcat tacattcgta atcacgcctg taaagggatt aaagtggatc aggtactgga
    13621 tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac
    13681 catccatgcc atgattcatg ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac
    13741 caccttgtcg atcaatgaga tatcgcaaat gtgcggttat ccatcgctgc aatatttcta
    13801 ctctgttttt aaaaaagcat atgacacaac gccaaaagag tatcgcgatg taaatagcga
    13861 gatcatgttg tagcgatgat gagaattgtc ggcgtcacat cagggaactc agcacagcca
    13921 cctgatgcga gcaaaagcct tatccgaccg acaaatcaga taaggctcaa ggaaatgcaa
    13981 ttacatatgc gccgcgatta accgttggtt atcctggtac attgcgaaca ggtagttatt
    14041 ataactcttc cctttggtcg agtagccctt cagcttgtga atcatcgctg tggcagtcac
    14101 ttcctgatcc gctttacgca gctgcgcacg cgatttacgg aacgaagagt aagccgggtg
    14161 cgtattcagg ttagtgacat aggcgctcac cgattctttg acagaactaa actgtgagta
    14221 ccctttcact ttacccggcg cattggtaca acgtcctttc atgcatttca tgccgaacag
    14281 attgttattg ttgcgcgcca gcttcgacgt tccccaaccg ctttctgctg cagccatcgt
    14341 cgccaccata ctggtaggga taatgtctac gcgttcaagc aaggtattcc acgggatttt
    14401 tcgcgtatta ccagaccact tcaccttgta gcgtttggcg atgtctttca gacgcgcacg
    14461 ctcagcaggt gaccattggc cctgatactg ttttgaaatc agccagttac gttctgcagt
    14521 aatggctgca ttttggctgg taatgtaagg cattacggtc cggagaaacg cctttttcct
    14581 tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg cactattgcg
    14641 agaatactct tgtttactgc ttacctgttt attactagct ttagttaagt gggacttttg
    14701 actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca gagtgagtaa
    14761 cataagaatc atcgccccat atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg
    14821 gatttaatca ttccaacacc ttatattttt cacaaatttg agagttgaat ctcaaatcat
    14881 atcaaaaata gctgtcaaga gcaccccaag gaatagtcca aatctgaaac tatgtcacgt
    14941 gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg gggttatcgc aaaattcagc
    15001 cgttttttgc gcgagatcgc tcacccttgc ttctcatcct gtggacttac cgctcaggga
    15061 tgagttttgt ctggcttatc gctggcaaac tgtctgaaat cgcagcaata aggactcatc
    15121 caccatgaaa ctcgccgcct gttttctgac actccttcct ggcttcgccg ttgccgccag
    15181 ctggacttct ccggggttcc ctgcctttag cgaacaggga acgggaacat ttgtcagcca
    15241 cgcgcagttg cccaaaggta cgcgtccact cacgctaaat tttgaccaac agtgctggca
    15301 gcctgcagat gcgataaaac tcaatcagat gctttccctg caaccttgta gcaacacgcc
    15361 gcctcaatgg cgattgttca gagacggcaa atatacgctg caaatagaca cccgctccgg
    15421 tacgccaaca ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa acctggtccg
    15481 tgaatgcccg aaatgggatg gattaccgct cacgctggat gtcagcgcca ctttcccgga
    15541 aggagccgcc gtacgggatt attacagcca gcaaattgcg atagtgaaga acggtcaaat
    15601 aacgttacaa cccgctgcta ccagcaacgg tttactcctg ctggaacggg cagaaactga
    15661 cgcccctgcc cctttcgact ggcataacgc cacggtttac tttgtgctga cagatcgttt
    15721 cgaaaacggc gatcccagta atgaccagag ttacggacgt cataaagacg gtatggcgga
    15781 aattggcact tttcacggcg gcgatttacg cggcctgacc aacaaactgg attacctcca
    15841 gcagttgggc gttaatgctt tatggataag cgccccattt gagcaaattc acggctgggt
    15901 cggcggcggt acaaaaggcg atttcccgca ttatgcctac cacggttatt acacacagga
    15961 ctggacgaat cttgatgcca atatgggcaa cgaagccgat ctacggacgc tggttgatag
    16021 cgcacatcag cgcggtattc gtattctctt tgatgtcgtg atgaaccaca ccggctatgc
    16081 cacgctggcg gatatgcagg agtatcagtt tggcgcgtta tatctttctg gtgacgaggt
    16141 gaaaaaaacg ctgggtgaac gctggagcga ctggaaacct gccgccgggc aaacctggca
    16201 tagctttaac gattacatta atttcagcga caaaacaggc tgggataaat ggtggggaaa
    16261 aaactggatc agaacggata tcggcgatta cgacaatcct ggattcgacg atctcactat
    16321 gtcgctggcc tttttgccgg atatcaaaac cgaatcaact accgcttctg gtctgccggt
    16381 gttctataaa aacaaaacgg atactcacgc taaagtcatc gaaggcttta cacctcgcga
    16441 ttacttaacc cactggttaa gtcagtgggt ccgcgactat gggattgatg gttttcgggt
    16501 cgataccgcc aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg aagccagcgc
    16561 cgcgcttcgc gaatggaaaa aagctaaccc cgacaaagca ttagatgaca aacctttctg
    16621 gatgaccggt gaagcctggg gccacggcgt gatgcaaagt gactactatc gccacggctt
    16681 cgatgcgatg atcaatttcg attatcagga gcaggcggcg aaagcagtcg attgtctggc
    16741 gcagatggat acgacctggc agcaaatggc ggagaaattg cagggtttca acgtgttgag
    16801 ctacctctcg tcgcatgata cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt
    16861 attactatta gcgccaggcg cggtacaaat cttttatggc gatgaatcct cgcgtccgtt
    16921 cggtcctaca ggttctgatc cgctgcaagg tacacgttcg gatatgaact ggcaggatgt
    16981 tagcggtaaa tctgccgcca acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg
    17041 ccatcccgca attggcgcgg gcaaacaaac gacactttcg ctgaagcagg gctacggctt
    17101 tgttcgtgag catggcgacg ataaagtgct ggtcatctgg gctgggcaac agtgactttt
    17161 ccggcttccc gttcgtcagt acctcgggaa gccgccaacc aggataaaat gtcagcccta
    17221 atcagcgttg caggataaag caccgctcac tctccaacag accgatttgc accccagcaa
    17281 atgtagcgtt attgttacct tccttgctac agagttcgac agatatcccg ctatgacatt
    17341 ctcccttttt ggtgacaaat ttacccgcca ctccggcatt acgctgctga tggaagatct
    17401 gaacgacggt ttacgcacgc ctggcgcgat tatgctcggt ggcggtaatc cggcgcagat
    17461 cccggaaatg caggactact tccagacgct actgaccgac atgctggaaa gtggcaaagc
    17521 gactgatgca ctgtgcaatt acgacggtcc acaggggaaa acggagctac tcacactgct
    17581 tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa gcacagaata ttgcactaac
    17641 aaacggcagc cagagcgcgt ttttctactt atttaatctg tttgccggac gccgtgccga
    17701 tggtcgggtc aaaaaagtgc tgttcccgct ggcaccggaa tacattggct acgctgacgc
    17761 cggactggaa gaagatctgt ttgtctctgc gcgtccgaat attgaactgc tgccggaagg
    17821 ccagtttaaa taccacgtcg attttgagca tctgcatatt ggcgaagaaa ccgggatgat
    17881 ttgcgtctcc cggccgacga atccaacagg caatgtgatt actgacgaag agttgctgaa
    17941 gcttgacgcg ctggcgaatc aacacggcat tccgctggtg attgataacg cttatggcgt
    18001 cccgttcccg ggtatcatct tcagtgaagc gcgcccgcta tggaatccga atatcgtgct
    18061 gtgcatgagt ctttccaagc tggggctgcc tggctcccgc tgcggcatta tcatcgccaa
    18121 tgaaaaaatc atcactgcca tcaccaatat gaacggcatt atcagcctgg cacctggcgg
    18181 tattggtccg gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc gcctgtctga
    18241 aacagtcatc aaaccgtttt actaccagcg tgttcaggaa actatcgcca tcattcgccg
    18301 ctatttaccg gaagatcgct gcctaattca taaaccagaa ggagccattt tcctctggct
    18361 ttggtttaag gatttgccca ttacgaccga gcagctctat cagcgcctga aagcacgcgg
    18421 cgtgctgatg gtgccggggc acaacttctt cccagggctg gacaaaccgt ggccgcatac
    18481 gcatcaatgt atgcgcatga actacgtacc agagccggag aaaattgagg cgggggtgaa
    18541 gattctggcg gaagagatag aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc
    18601 gctacgctta ttaggcctac atgatttctg caatatattg aatttgcaag aatttgtggg
    18661 ccggataagg cgtttacgcc gcatctggca taaacaaagc gcactattcc ggcgttgaga
    18721 aacgccggaa aaacgtttca aacaggctgc tgccgtagcc gatgccgctg cactttctcg
    18781 acatccatgc actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc
    18841 cgatgccagc acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc
    18901 atcgcaccaa acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca
    18961 acgaaaatat gcccatgttc gcggcttatc gcgttaacag ggcagacatt cgcgcacggt
    19021 gcatcttcac actgatgaca ggctactgcc gtggtccagc tgtggtcttt aatgacacga
    19081 atacgggaaa taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac
    19141 actgcgcaag ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata
    19201 aaccggttca tgatcactcc tttgcactaa aacagcagag tgccagagag cgcgtcccta
    19261 ctgctttgat ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga
    19321 tcgcgccctg gtcgtccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt
    19381 cacgcagtgg tttcaggaga tttttctccc ccacctgttt cagacgtgat gtcgaaagcg
    19441 aaatcgacac ggcgtacggc acccgcccat gaatattaaa caccggaaca gcaatacagg
    19501 agacgccgag ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca
    19561 gttcgtcgaa catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat
    19621 gctgatggtt ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct
    19681 tgcccattgc ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca
    19741 tcccggttgt gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga
    19801 agttaatggt ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa
    19861 tgatattcag cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt
    19921 aactccctgc ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc
    19981 gatggacggt actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt
    20041 ttggatagtt gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg
    20101 ctggacgctc tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca
    20161 ttttgtcgcg tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat
    20221 ttataaccac ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa
    20281 gcgatcgata tatttgaaat caagtttcgc atattgaaat tttaagccaa aaaagcgatc
    20341 aaaaaaacaa ggaagcctgg gatgaaagtg acatttgagc agttaaaagc agcctttaat
    20401 cgggtcttaa tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc
    20461 gcccgcacca ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt
    20521 caacaactgg aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc
    20581 ggcgcaattg aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg
    20641 atggatcgcg ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat
    20701 gccaaccact ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt
    20761 ggcatttgct ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc
    20821 attggcacta acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg
    20881 tcgatgtcga tgttctctta cggcatgtta gaagttaacc gcctggcggg ccgtcagctc
    20941 ccggtcgatg gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag
    21001 aagaatcgcc gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg
    21061 gatatgatcg ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac
    21121 agcgacgaat acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac
    21181 ggtcccaccc gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc
    21241 gctgacgaaa atcaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa
    21301 aaccgccgta acggtattac cgttgatgac agcgtgtggg ccaaaatcca ggcgttataa
    21361 ggagataagt catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg
    21421 ccattgaaaa ggcgctcgat tttctgcgcg ctaccgattt caacgccctg gagccgggcg
    21481 ttgtcgaaat cgacggcaaa aatatttatg cgcaaattat cgatttaacc acacgcgaag
    21541 cggtggaaaa ccgtccggaa gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg
    21601 gtgaagagaa aatcggcatt gctattgata cgggaaataa taaagtcagc gaatcactat
    21661 tagagcagcg cgatattatt ttttatcatg acagtgaaca tgaatcattt atagaaatga
    21721 taccgggcag ctacgcaatt ttctttccgc aggatgttca ccgaccaggt tgtattttgc
    21781 aaacagcctc tgagatacga aaaattgtgg ttaaagtcgc attaacggcg ctgaattaat
    21841 cgaataatcg tctacgccag aacgactggg taatgttatt gctctaacta tattaataat
    21901 ttaggttgct gcacatgcaa cttgaattat gacagacata gcctcaagga atagctatga
    21961 aaaaaatact tgaagcaata ctggcgatta atctcgccgt actttcctgt attgtgttta
    22021 taaacattat tttaagatat ggttttcaga caagcatttt atctgtagat gaattgtcac
    22081 gttatttatt tgtctggtta acatttattg gcgcgattgt ggcttttatg gataacgccc
    22141 acgttcaggt cacctttctg gtggagaaac tctcccccgc atggcaacga cgagtcgctc
    22201 tggttaccca ttccttaatc ttatttattt gtggcgcact ggcctggggc gcaacgctaa
    22261 aaacaattca ggactggagc gattattcac cgatcctcgg tttacccatc ggcctgatgt
    22321 atgccgcttg tctgcccacc agcctcgtta tcgcattctt tgaactgcgt catttatatc
    22381 aactcatcac gcgtagcaat tctctaacgt cgccaccgca aggagcttaa tcatggctgt
    22441 gctgattttt ctgggctgtc tgttgggtgg tattgctatc ggtttgccta ttgcctgggc
    22501 actgttgttg tgcggggcgg cgttaatgtt ctggctggac atgtttgatg tccagatcat
    22561 ggcacaaacg ctggtgaacg gagccgatag cttctccctg ctggcgattc cgttctttgt
    22621 gctggcgggt gaaatcatga atgcgggcgg cttgtcaaaa cgcattgttg acctgccgat
    22681 gaaactggtg gggcataaac caggcgggct gggctacgtc ggcgtgctgg cagctatgat
    22741 tatggcgagc ctttctggtt ctgccgttgc ggataccgcc gccgttgccg ctctactggt
    22801 gccgatgatg cgcagcgcca actacccggt caaccgggcg gcggggctga ttgcctctgg
    22861 cggcattatc gcgccaatta ttcccccttc cattccgttt attatcttcg gcgtttccag
    22921 cggattatcc atcagtaagc tgtttatggc gggcattgcc cctggcatga tgatgggcgc
    22981 aacgctgatg cttacctggt ggtggcaggc cagtcgcctt aatttacccc gccagcaaaa
    23041 agcaacgatg caggaaatct ggcactcttt tgtctccggt atctgggcgc tgtttcttcc
    23101 agtcatcatt attggcgggt ttcgctccgg tctgtttacc cctaccgaag ccggggccgt
    23161 tgctgctttc tatgcgctgt ttgtcgccac agttatttac cgtgaaatga ccttcgccac
    23221 actttggcat gtactcattg gcgcggcgaa aaccacctca gtggttatgt ttctggtggc
    23281 ctcggcacaa gtttccgcct ggctgattac cattgctgaa ctgccgatga tggtttctga
    23341 tttactgcaa ccgttggtcg attcaccgcg tctgctgttt atcgtcatta tggtggctat
    23401 cctgattgtc ggcatggtca tggatttaac gccgacggta ttaattctta ccccggtatt
    23461 aatgccttta gttaaagagg caggaatcga tccgatttat ttcggtgtca tgtttatcat
    23521 taactgctca atcggcttaa ttacaccgcc tatcggtaat gtacttaacg ttatttcagg
    23581 ggtggcaaaa ctcaaattcg atgatgcagt cagaggcgtt ttcccttacg tcctggtttt
    23641 atattcatta ttagtcgtat ttgtttttat tcccgatctc atcatcctcc ctttgaaatg
    23701 gattaattaa aaggaaaata ttatgaaatt acgctctgta acctacgcat tattcattgc
    23761 tggcctggct gcattcagca catcttctct ggcggcgcaa tctttacgtt tcggttatga
    23821 aacctcacaa accgactcgc aacatattgc ggcgaaaaaa ttcaatgatt tattgcagga
    23881 gagaaccaaa ggcgagctga aattaaaatt gtttccggat agcaccctcg gtaacgcgca
    23941 ggcgatgatc agcggcgtac gtggcggcac catcgatatg gaaatgtccg gctcgaataa
    24001 ctttgccggg ttatcaccag tgatgaactt gcttgatgtc cctttcctgt tccgcgatac
    24061 cgctcacgcg cataaaacgc tcgacggcaa agtcggtgat gatctgaaag cctcacttga
    24121 aggtaaagga ctaaaagtac tggcctactg ggaaaacggc tggcgcgatg tcaccaactc
    24181 gcgcgcaccg gttaaaaccc ccgccgacct gaaagggctg aaaattcgca ccaacaatag
    24241 cccgatgaat atcgccgcat tcaaagtctt tggcgctaac ccgatcccga tgccgtttgc
    24301 cgaagtctat accgggctgg aaacccgcac tatcgacgct caggaacacc cgatcaacgt
    24361 cgtctggtca gcaaaatttt tcgaagtgca gaagtacctt tctctgacgc accacgccta
    24421 ttccccgctt ctggtggtga tcaacaaagc gaagtttgat ggcttaagcc cggagttcca
    24481 gcaagcgcta atttcatctg cgcaggaagc gggtaactac cagcgcaaac tggttgccga
    24541 agatcagcaa aaaatcatcg acggcatgaa agaagcgggc gtggaagtca tcaccgatct
    24601 cgaccgcaaa gcctttagcg acgctctggg taatcaggtt cgcgacatgt ttgtaaaaga
    24661 tgttccgcag ggtgctgatc tactgaaagc cgtggatgag gtgcaataaa ccatgacgca
    24721 atactggctg gggttagatt gtggcggtag ctggctgaaa gccgggctgt atgaccgcga
    24781 aggccgggag gcaggcgtgc agcgcctgcc gctgtgcgca ttaagcccgc agccaggttg
    24841 ggcagagcgc gatatggcag aactgtggca atgctgcatg gctgtcattc gcgaactgct
    24901 tacccattcc ggcgttagcg gggaacaaat tgtcggtatc ggtatctccg cacaaggaaa
    24961 aggcttgttt ttgctggata aaaacaacaa gccgctcggt aacgccattt tatcctcgga
    25021 tcgccgggcg atggaaatcg ttcgtcgctg gcaggaagat ggcatcccgg aaaaactcta
    25081 cccgctgacc cgacaaacct tgtggaccgg gcatccggta tcgctgttac gttggcttaa
    25141 agagcacgaa ccggaacgct acgcgcaaat tggctgcgtg atgatgacgc acgactacct
    25201 gcgctggtgt ttaaccggcg tcaaaggctg tgaagagagc aatatttccg agtccaatct
    25261 ctacaacatg agtcttgggg aatatgaccc gtgcctcacc gactggctgg ggattgctga
    25321 aatcaatcac gccctgccgc ctgttgtcgg atctgccgaa atctgcgggg agatcaccgc
    25381 tcagacagcc gtactgaccg gtctgaaagc gggtacgccc gttgtcggcg gcctgtttga
    25441 tgtggtttcc accgcactct gcgccgggat cgaagacgaa tttaccctta atgcggtgat
    25501 gggcacctgg gcggtgacca gcggcataac ccgcggttta cgtgacggtg aagcgcatcc
    25561 gtatgtctat ggtcgctacg ttaacgatgg tgaatttatc gttcacgaag ccagccccac
    25621 ctcttccggc aacctcgaat ggtttaccgc acagtgggga gaaatctcgt ttgatgagat
    25681 caaccaggcc gttgccagct tgccgaaggc cggtggcgat ctctttttcc tgccgttcct
    25741 gtacggcagc aacgccggac tcgagatgac cagcggtttc tacgggatgc aggccattca
    25801 cacccgcgca cacctgttgc aggccattta tgaagggggg gtgttcagcc atatgaccca
    25861 cctcaaccgg atgcgcgaac gttttactga tgtgcacacc ctgcgcgtca ctggcggccc
    25921 ggcgcactcc gatgtctgga tgcaaatgct ggcggacgtc agcggtctgc gtatcgagct
    25981 gccgcaggtg gaagaaaccg gctgttttgg cgcggcactt gccgcccgtg tcggcaccgg
    26041 agtttatcgc gatttcagcg aagctcaacg tgacttacag cacccggtac gcaccctgct
    26101 gccggatatg accgcccatc agctttacca acaaaaatac caacgctatc agcatctcat
    26161 tgccgcactt cagggctttc acgcccgcat caaggagcac atattatgag ccgaccactt
    26221 ctgcaactgg cactcgacca ctcatcactt gaagccgcac agcgcgacgt gacgcagtta
    26281 aaagacagcg tcgatattgt cgaagcgggc accattctct gtttaaacga agggcttggc
    26341 gcggtgaaag ccttgcgcga acagtgccct gacaaaatca tcgttgccga ctggaaggtc
    26401 gccgacgctg gtgaaacgct cgcgcaacag gcatttggcg caggcgctaa ctggatgacc
    26461 atcatctgcg ccgcaccgct cgcgacggtg gaaaaaggcc acgcgatggc gcaacgctgc
    26521 ggtggtgaaa ttcagatcga gctgttcggt aactggacgc tggacgacgc ccgcgactgg
    26581 catcgtattg gcgtgcggca ggcgatttat catcgcggtc gtgatgcaca ggccagcggg
    26641 caacagtggg gcgaagccga tctggcacgc atgaaggcgc tttccgatat cggccttgag
    26701 ctttccatta ccggcgggat tacccctgct gacctgccgc tgtttaaaga tattcgcgtg
    26761 aaagcgttta ttgccgggcg agcactggca ggctcagcaa atccggcgca ggtggctggc
    26821 gatttccatg cgcaaatcga cgctatctgg ggaggcaagc gtgcgtaatc atccgttagg
    26881 gatttatgaa aaggcgctgg cgaaagatct ctcctggccg gagcggctgg tgctggcaaa
    26941 aagctgcggt tttgattttg tcgaaatgtc ggtggatgaa accgacgaac ggctttcacg
    27001 tcttgactgg agcaccgcac aaaggacttc gctggttgcc gcgatgatcg aaacgggtgt
    27061 tgggatcccg tcgatgtgcc tgtccgcaca tcgtcgcttt ccttttggta gccgtgacga
    27121 cgcggtacgc cagcgagcac gggagatcat gagcaaagcg attcgtctgg cacgcgatct
    27181 cggcatacgc accatccagc tggcgggcta cgacgtctat tacgaagacc acgatgaagg
    27241 cacccggcaa cgttttgctg aagggctggc gtgggcggtc gaacaggcgg cggcatcaca
    27301 agtcatgcta gcggtggaga tgatggacac cgcgtttatg aactccatca gtaaatggaa
    27361 aaagtgggac gagatgctcg cctcgccgtg gttcaccgtc tacccggacg tcggcaacct
    27421 cagcgcctgg ggcaatgatg ttcccgccga actgaaactg ggcattgacc gtatcgccgc
    27481 gatccacctg aaagacaccc agccagttac cgagcaaagt cccggacagt tccgcgacgt
    27541 gccgtttggc gaagactgcg tcgatttcgt tggcatcttc aaaacgctgc ataaactgaa
    27601 ctatcgcggt tctttcctga ttgagatgtg gaccgaaaaa gccaaagagc cggtgctgga
    27661 gattattcag gcgcggcgtt ggattgaagc gcgtatgcag gaggctggat ttatatgtta
    27721 gagcaactga aagccgacgt gctggcggcg aatctggcgc ttcccgctca ccatctggtg
    27781 acgttcacct ggggcaatgt cagcgcggta gacgaaacgc ggcaatggat ggtaatcaaa
    27841 ccttccggcg tcgagtatga cgtgatgacc gccgacgata tggtggtggt tgagatagcc
    27901 agcggcaagg tggtggaagg tagcaaaaag ccctcgtccg acacgccaac gcatctggcg
    27961 ctttaccgtc gctacgccga aatcggtggc attgtgcata cccactcacg ccacgccacc
    28021 atctggtcgc aggccgggct ggatctcccc gcctggggca ccacgcacgc tgattatttc
    28081 tatggtgcca tcccctgcac gcgacagatg accgcagagg agattaacgg cgaatatgaa
    28141 tatcagaccg gcgaagtgat cattgaaacc ttcgaagaac gtggcaggag tccggcacaa
    28201 atcccggcgg tgctggtgca ttctcacggc ccgttcgcat ggggtaaaaa cgccgccgat
    28261 gccgtgcata acgccgtagt actcgaagaa tgcgcctata tgggcctatt ctcgcgccag
    28321 cttgcgccgc agctccctgc gatgcaaaac gaactgctgg ataagcacta cctgcgtaag
    28381 catggggcca atgcctatta cgggcagtaa tccctcacgc cggggcttca tcgccccggc
    28441 actacgaatt gatatgttcc ttgctgtaac gccgcttcca cgctgctggc gttaaaccgg
    28501 tgtgcttgcg aaaaatctgc cggaaatagc caacctcatt aaaccgtatt gcatggagca
    28561 ggccgacaac gtcgagtcac tgcataacct gttgctgccc gccgatgcgc catttacgac
    28621 atttgaaggc aagggattgt tcagccataa gatcctgatt cagacgccag gttcccggta
    28681 tgaacagagc aatccagagc aacaaccttt gtggcattac gacggagcac cagccgcacc
    28741 gacatccacc ggtgaattaa aaacgccagc tcacgccggt gacaaaggta aaactctcat
    28801 tacgatcaat catcgggctg tcggtaatct cttccggcag ctcgctgtat ccggcgctcg
    28861 ccatcaatac tacgtgctct cctatcgggt attttgccgt caggctgaca tagggcaccc
    28921 aggcatcctg cgcggaataa cttgccagac cgctacgacg ggactcactc tctgaaatgc
    28981 catagtaata gtcactgaaa ttctcgtcat aatagagaac gcccagcgca ggtgtcagcg
    29041 acaggcgacc tatctgcatt ttgtggaaaa ccgatagctc ccccacccag ccgttgctgt
    29101 tatccagaac gtccgcagct gcagaggctt tcacggttcc ccaacgctcg tggtgatacc
    29161 aggcaacccc cgccatagcc gtactatcac gcttatcaac ttgttgcatg gcatgatcgt
    29221 cattatcggc agggtcaaat tccagcggca tccaggatgc ggtcaggcta agttcatttt
    29281 tttcactttg cgacagaata aaacctaacg tggtctgacg aacataaaaa gaatcacctt
    29341 cataactaat caggggaatt gcctttgtat tttcattata accgcgataa ggcgattcat
    29401 tataagccgc accggcacca atggataatt cagaagcagt tgcgcttgcc ataaaaggca
    29461 acgcaaataa cgccacaata ttgcggttaa ttaacatgtc cggtattcca ttttaaaata
    29521 agataaaaaa gagtcggcat agtaatgctt ac
//
LOCUS       DAZGCS010000064        29463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGCS010000064 DAZGCS010000000
VERSION     DAZGCS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.064"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(217..1866)
                     /gene="actP"
                     /locus_tag="ACHK01_002887"
     CDS             complement(217..1866)
                     /gene="actP"
                     /locus_tag="ACHK01_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO8097813.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1863..2177)
                     /gene="yjcH"
                     /locus_tag="ACHK01_002888"
     CDS             complement(1863..2177)
                     /gene="yjcH"
                     /locus_tag="ACHK01_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO8097814.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2377..4335)
                     /gene="acs"
                     /locus_tag="ACHK01_002889"
     CDS             complement(2377..4335)
                     /gene="acs"
                     /locus_tag="ACHK01_002889"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO8097815.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4727..6163
                     /gene="nrfA"
                     /locus_tag="ACHK01_002890"
     CDS             4727..6163
                     /gene="nrfA"
                     /locus_tag="ACHK01_002890"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO8097816.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6208..6774
                     /gene="nrfB"
                     /locus_tag="ACHK01_002891"
     CDS             6208..6774
                     /gene="nrfB"
                     /locus_tag="ACHK01_002891"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO8097817.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6771..7442
                     /gene="nrfC"
                     /locus_tag="ACHK01_002892"
     CDS             6771..7442
                     /gene="nrfC"
                     /locus_tag="ACHK01_002892"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO8097818.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGGRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7439..8395
                     /gene="nrfD"
                     /locus_tag="ACHK01_002893"
     CDS             7439..8395
                     /gene="nrfD"
                     /locus_tag="ACHK01_002893"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO8097819.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8415..10133
                     /gene="nrfE"
                     /locus_tag="ACHK01_002894"
     CDS             8415..10133
                     /gene="nrfE"
                     /locus_tag="ACHK01_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO8097820.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10126..10509
                     /gene="nrfF"
                     /locus_tag="ACHK01_002895"
     CDS             10126..10509
                     /gene="nrfF"
                     /locus_tag="ACHK01_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO8097821.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10506..11102
                     /gene="nrfG"
                     /locus_tag="ACHK01_002896"
     CDS             10506..11102
                     /gene="nrfG"
                     /locus_tag="ACHK01_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO8097822.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11444..12757
                     /gene="gltP"
                     /locus_tag="ACHK01_002897"
     CDS             11444..12757
                     /gene="gltP"
                     /locus_tag="ACHK01_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO8097823.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12835..13524)
                     /gene="yjcO"
                     /locus_tag="ACHK01_002898"
     CDS             complement(12835..13524)
                     /gene="yjcO"
                     /locus_tag="ACHK01_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO8097824.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13618..15765)
                     /gene="fdhF"
                     /locus_tag="ACHK01_002899"
     CDS             complement(13618..15765)
                     /gene="fdhF"
                     /locus_tag="ACHK01_002899"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15346..15348),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO8097825.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15963..17429)
                     /gene="mdtP"
                     /locus_tag="ACHK01_002900"
     CDS             complement(15963..17429)
                     /gene="mdtP"
                     /locus_tag="ACHK01_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO8097826.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17426..19477)
                     /gene="mdtO"
                     /locus_tag="ACHK01_002901"
     CDS             complement(17426..19477)
                     /gene="mdtO"
                     /locus_tag="ACHK01_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO8097827.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEVRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     REERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19477..20508)
                     /gene="mdtN"
                     /locus_tag="ACHK01_002902"
     CDS             complement(19477..20508)
                     /gene="mdtN"
                     /locus_tag="ACHK01_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO8097828.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20527..20802)
                     /gene="ytcA"
                     /locus_tag="ACHK01_002903"
     CDS             complement(20527..20802)
                     /gene="ytcA"
                     /locus_tag="ACHK01_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO8097829.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(21011..22996)
                     /gene="yjcS"
                     /locus_tag="ACHK01_002904"
     CDS             complement(21011..22996)
                     /gene="yjcS"
                     /locus_tag="ACHK01_002904"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO8097830.1"
                     /translation="MNNSRLFRLSKIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(23269..24198)
                     /gene="alsK"
                     /locus_tag="ACHK01_002905"
     CDS             complement(23269..24198)
                     /gene="alsK"
                     /locus_tag="ACHK01_002905"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO8097831.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(24182..24877)
                     /gene="alsE"
                     /locus_tag="ACHK01_002906"
     CDS             complement(24182..24877)
                     /gene="alsE"
                     /locus_tag="ACHK01_002906"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO8097832.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNKATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(24888..25868)
                     /gene="alsC"
                     /locus_tag="ACHK01_002907"
     CDS             complement(24888..25868)
                     /gene="alsC"
                     /locus_tag="ACHK01_002907"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO8097833.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(25847..27378)
                     /gene="alsA"
                     /locus_tag="ACHK01_002908"
                     /pseudo
     CDS             complement(25847..27378)
                     /gene="alsA"
                     /locus_tag="ACHK01_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            complement(27505..28440)
                     /gene="alsB"
                     /locus_tag="ACHK01_002909"
     CDS             complement(27505..28440)
                     /gene="alsB"
                     /locus_tag="ACHK01_002909"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO8097834.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(28499..29389)
                     /gene="alsR"
                     /locus_tag="ACHK01_002910"
     CDS             complement(28499..29389)
                     /gene="alsR"
                     /locus_tag="ACHK01_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO8097835.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALSVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         6819 a         7684 c         7779 g         7181 t
ORIGIN      
        1 aagaggaaaa aacatattat ttaaaccatt atagcgcata aaatatcctt ataattaaca
       61 agaaacaaaa ggcatattct ctgcattatc attttctacg actgtcaaaa atcgctcatt
      121 ttttaatgag tttatttgtt taatattatg ggaaaaggtg atgcatgttg gagaggaaga
      181 gtattccccg gtcaggcgac cggggaaggg gagagattaa tgtgcgcggc cttgctcaac
      241 gccaaagccg gtctgggagc ggataaactg tgcacggaac agttcacgct cgcgcgcacc
      301 ttccgctgag ttatcggttg ccgagaagaa ccagatgccg aggaatgcca cgctgataga
      361 gaacagcgcc gggtattcat acgggaagat ggctttttcg tgaccaagga tctgtaccca
      421 aatcgtcggg ccgaggatca tcagtaccac tgcggtaatc agccccagcc agccacccat
      481 catcgcgcca cgagtggtca gtttcgacca gtacatagaa agcagaatga tcgggaagtt
      541 acagctcgct gcgatggcaa acgccaggcc taccatgaag gcgatgttct gattctcaaa
      601 cagcacgccg aggataatgg caatcacacc taaaatcagc acggtgattt tcgatacccg
      661 cagttcttca cgttcggtcg cgcctttttt gaagacgtta gcgtacaggt catgcgaaac
      721 cgctgatgcg cccgccagcg tcagaccagc aaccaccgca agaatggtgg caaaagcaac
      781 cgctgaaata aaaccgagga acaggttgcc gcccactgca ttcgccaggt gaacggccgc
      841 catgttgtta ccaccaatca gatggcccgc cgcgtcttta tattccggat tcgcaccaac
      901 cagcatgatc gcgccgaagc cgataataaa ggtcagaata tagaagtagc ccataaaccc
      961 ggtggcgtag aacacgctct tacgtgcttc gcgggcatcg ctgactgtaa agaagcgcat
     1021 cagaatgtgc ggcaagcccg ccgtaccaaa catcagtccc agacccagag agagcgcgga
     1081 gatcggatct ttcaccagcc cgcccggctt catgatgtcg acacctttcg ggtgtaccgc
     1141 catcgcttca ctgaacagat tgttgaagct aaagccgacg tgtttcatca ccataaaggc
     1201 cataaagctg gcaccgaaca gcaacagcac ggctttgata atctgtaccc aagtggtcgc
     1261 cagcatgccg ccgaacagga cgtacatcat catcagcacg ccgaccagca ccaccgcaat
     1321 gtgatagtta aggccaaaca gcagctcgat cagtttacct gcgcccacca tctgggcgat
     1381 aaggtaaagc gccaccacca ccagagaacc acaggccgaa agaatacgaa tcggcccttg
     1441 tttcagacgg taagaggcca catcggcaaa ggtgtagcgc cccaggttac gcagacgttc
     1501 ggcaatcagg aacaaaatga tcggccagcc caccaggaag cccagcgagt aaattaagcc
     1561 gtcatagccg gaggtaaaca ccagcgcgga gatccccaag aatgaggcgg cggacatata
     1621 gtccccggca atcgccagcc cgttctggaa gccagtgata ttgccgcctg cggtgtagta
     1681 gtcgctacga gaacgtacgc gttttgatgc ccagtaggta atgccgagcg taaacacgac
     1741 gaaaatcagg aacataataa tcgcctgcca gttcgttggc tggcgctcta cggccccgct
     1801 aatagcatcc gcggcgttag ctgcgaaagg gagtgtggcg gcaagcgccg tcagaactct
     1861 tttcatgatg cttgtacctc atgcaggact tcattattaa gacggtcgaa ttcgccgttc
     1921 gcccgccaga tgtagatacc ggtgagaaca aaggagatca caatcactcc aacaccaatt
     1981 ggaataccgc gtgtgacgct ggtgttcgga ttcagcggcg tgcccagcca gccgggcgcg
     2041 aaggcgatca gtaaaataaa gccgatataa actgccagca taataatcga caggatggtg
     2101 gcaaaccgtt gccgtttttc gactaactcc ctgaaatgcg cattgtcttc tatccgctga
     2161 taaatagtgc cattcatcac agattctcca gaggtaatgt agggattgtt ttaattcccg
     2221 ctcccttatg ggagaaggtt aacgctcggg taacccttgc cgaatgtagg ccggataagg
     2281 cgtttacgcc gcatccggca atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct
     2341 acaaaccgtt accgactcgc atccggcaat tgtgggttac gatggcatcg cgatagcctg
     2401 cttctcttca agcagcttct cgactacgcc aggatcggca agcgtcgagg tatcgcccag
     2461 gttgctggta tcgcccgccg caattttgcg cagaatacgg cgcataattt tgccggagcg
     2521 ggttttaggc agggagtcgg tccagtgcag cacgtctggc gtcgccagcg ggccaatctc
     2581 tttacgcacc cagttgcgga cttctgcgta cagttctggt gacggttcct ccccgtgatt
     2641 aagcgtgacg taggcgtaga tcgcctgacc tttaatattg tgcggaatac ctactacggc
     2701 ggcttcggca atcttcggat gcgccaccag cgccgactca atctctgccg tccccagacg
     2761 gtgaccggag acgttcagca cgtcgtccac acgcccggtg atccagtaat agccatcttc
     2821 atcgcgacgc gcgccgtcgc cgctgaaata catatttttg aaggtggaga agtaggtctg
     2881 ttcaaaacgt tcgtgatcgc caaacagcgt acgcgcctga cccggccagg agtcggtgat
     2941 caccaggctg ccttcggtag cgccttccag cgggttacct tcgttatcga ccagcgccgg
     3001 ttgcacgccg aagaacggac gtgttgccga accggctttc agctcggtag cgccaggcag
     3061 cggcgtgatc atgaaaccgc cagtttcggt ctgccaccag gtatcgacca ccggacattt
     3121 ctcgttgccg atttttttcc agtaccactc ccacgcttcc gggttaattg gctcgcccac
     3181 ggaaccgaga atgcgcagcg acgaacggtc ggtgccttcg atcgctttat cgccttccgc
     3241 catcagcgcg cggatcgccg tgggtgcggt atagagaata ttgacctgat gcttgtccac
     3301 cacctgcgcc atacgggcag gcgtcggcca gttgggtacg ccttcaaaca tcagcgtggt
     3361 cgcaccgcag gccagcgggc cgtacagcaa gtaactgtgt ccggtcaccc agcccacatc
     3421 ggcggtgcac cagtagatat cacccggatg ataatcaaag acatatttaa aggtcagcgc
     3481 cgcgtacacc agataaccgc cggtagtatg cagcacacct tttggcttac cggtagaacc
     3541 ggaggtgtag agaataaaca gcggatcttc ggcgttcatc tcttccgcct ggtgctgatc
     3601 gctcgcttgc tcaaccaggt cgtgccacca caggtcgcgc ccttcctgcc agtcaatttt
     3661 cccgccagta cgcttcagta ccaccacatg ctctacgctg gtgacgttcg ggtttttcag
     3721 cgcgtcatca acgtttttct tcagcggaat actgcgcccg gcacgcacac cttcgtcgga
     3781 agtgatcacc agtcgtgagt tggaatcaat aatgcgcccg gcaacggctt ccggcgagaa
     3841 gccgccgaaa atcaccgaat gcaccgcgcc aatgcgggcg caggccagca tcgcaaccgc
     3901 ggcttccggc accatcggca tataaatcgc caccacatca ccttttttaa tgcccagctc
     3961 gagcagggta ttggcgaagc ggcagacgtc gcggtgcagc tctttatagc tgatatgttt
     4021 gctctggctg gcgtcgtcgc cttcccagat gatggcggtg cgatcgccgt tttcttgcag
     4081 atggcggtca aggcagtttg ccgccagatt cagcgtgccg tcctcgtacc atttaatgga
     4141 cacattaccg ggggcaaagg aggtattttt caccttctgg tacggtttga tccagtcgag
     4201 aatttttccc tgttcgcccc agaaggtatc aggtacgtta atagattgtt gatacatcgc
     4261 ctcgtactgc tgagggttta tcaggcaacg gtctgcgatg ttggcaggaa tggtgtgttt
     4321 gtgaatttgg ctcatgcttt tgttctcctt gtgggatgtt aataatatgt ggcataagcg
     4381 ttaaatgtag gggtattggc agttttgttt agtatttggg cgacagatca cgcaaaagta
     4441 gaattgtgca aataaacggc agggtaattt ttgaaggtca ccaacaaaag ttgattaatt
     4501 ctttgaggaa catgcagtta tgcatgctgt tgaaaaagag gaagatactg actaactcta
     4561 aagtggtatt ttacatgcac ttacaattga ttaaagacaa cattcacagt gtggttattt
     4621 gttacacata ggggcgagca atgtcatgac agtgtaggtg cggttactgt cgtgaaaaca
     4681 ataaaaaccg ccattgcaac aatggcgcaa ttcggatgaa gcccctatga caaggataaa
     4741 aataaacgca cgccgtatct tcagcttatt gattcctttt ttctttttca cttctgttca
     4801 cgctgaacaa acggcagctc ccgcaaaacc tgtaactgtg gaagcgaaga atgaaacctt
     4861 tgccccgcag catcccgatc aatatctctc ctggaaagcc acctcggaac agtcagagcg
     4921 tgttgacgcc ctggcggaag atccacggct ggtgatcctg tgggcggggt atcccttctc
     4981 gcgcgattac aacaagccgc gtggacatgc ctttgctgtg accgatgtgc gtgaaaccct
     5041 gcgtaccggt gcgccgaaaa acgctgaaga tggtccgcta ccgatggcgt gctggagttg
     5101 taaaagcccg gatgtggcgc gtctgatcca gaaagacggc gaagatggct acttccacgg
     5161 taagtgggcg cgcggcggcc cggaaatcgt caacaactta ggttgtgccg actgccataa
     5221 caccgcctca ccagagttcg ccaaaggcaa accggagtta accctttccc gtccgtatgc
     5281 ggctcgcgcg atggaagcca ttggtaaacc ttttgagaaa gccggacgtt tcgaccagca
     5341 atcgatggtt tgcggtcagt gccatgtgga gtattacttc gacggcaaaa acaaagcggt
     5401 taaattcccg tgggatgacg gcatgaaagt cgaaaatatg gagcagtatt acgacaaaat
     5461 tgccttctct gactggacta actccctgtc gaaaacgcca atgctgaaag cgcagcaccc
     5521 ggaatatgaa acctggacag cgggcattca cggtaaaaac aacgtgacct gtatcgactg
     5581 ccatatgcca aaagtgcaga acgccgaagg caaactctac accgaccata aaattggtaa
     5641 tccgtttgat aacttcgccc agacttgtgc gaactgccat acccaggaca aagctgcctt
     5701 gcaaaaagtg gtcgcggaac gtaagcagtc gattaacgac ctgaaaatca aggttgaaga
     5761 tcaactggtt cacgctcact tcgaagcgaa agcagcgctg gatgcaggcg cgacggaagc
     5821 cgaaatgaag ccgattcagg acgatatccg tcatgcccag tggcgttggg atctggcgat
     5881 cgcttcccac ggcattcata tgcacgcacc ggaagaaggt ttacggatgc tcggtactgc
     5941 gatggataaa gcggcggatg cacgcaccaa actggcgcgc ctgctggcga ccaaaggcat
     6001 cacccatgaa atcgagatcc cggatatctc gaccaaagag aaagcccagc aggccattgg
     6061 cctgaacatg gaacaaatca aggccgagaa gcaggacttc atcaaaacgg tgatcccgca
     6121 gtgggaagaa caggcacgta aaaatggtct gttaagccaa taaccccgtt ccgactcgca
     6181 aggggcggaa aacacaatgg agtgaatatg agcgtattac gttcgttatt aactgctggg
     6241 gtgctggcgt cgggcctgtt gtggagcctg aacggaatta ccgctacccc tgcggcgcag
     6301 gcatctggtg atcgctacga agttacccag cagcgtaacc cggatgccgc ctgtctggac
     6361 tgtcataagc cagatatcga aggtatgcat ggcaaacatg cttccgttat caacccgaat
     6421 aacaaactgc cggtcacctg caccaactgt cacggccagc catcaccgca acaccgcgaa
     6481 ggggtgaaag atgtgatgcg ctttaacgag ccgatgtaca aggtcggaga gcagaacagc
     6541 gtctgtatgt cctgtcatct gccggaacag ttgcaaaaag cgttctggcc gcacgatgtc
     6601 cacgtaacca aagtggcgtg cgccagctgc cattccctgc atccacagca agatacgatg
     6661 cagacgttaa gtgacaaagg acggattaag atttgcgtcg attgccacag cgatcagcgc
     6721 accaatccga actttaaccc ggcgtctgtt ccgttgctta aggagcagcc atgacctggt
     6781 ctcgtcgcca gtttctcacc ggcgtcggcg tgctggcagc cgtcagcggc accgcagggc
     6841 gcgtggtggc gaaaacgttg aatatcaatg gggggcgtta cggcatggtg catgacgagt
     6901 cgttatgcat cggctgtacc gcctgtatgg atgcttgtcg ggaagtgaac aaggtgccgg
     6961 aaggcgtctc gcgcctgatg attattcgta gcgagccgca gggcgaattt cctgatgtga
     7021 aatatcgctt cttccgtaag tcttgccagc actgcgatca tgcgccgtgc gttgacgtct
     7081 gcccgaccgg tgcgtctttt cgtgatgccg ctagcgggat tgtcgatgtt aatcctgatc
     7141 tctgcgtcgg ttgtcagtac tgcatcgccg cctgtccgta ccgcgtgcgc tttatccatc
     7201 cggtcacgaa gacggcggac aaatgcgatt tctgccgtaa gaccaatttg caggccggta
     7261 agctgcccgc gtgcgttgag gcttgcccga cgaaggcgct gacgtttggc aatctggacg
     7321 atcctaacag tgagatttcg caactgctgc gccagaagcc tacttaccgc tacaagctgg
     7381 cgctgggaac caaaccgaag ctgtaccgcg taccgtttaa atacggggag gtgagccaat
     7441 gacgcagact tccgcatttc attttgaatc gctggtgtgg gactggccga ttgccatcta
     7501 cctgtttttg attggtattt ctgccggtct ggtgacgctg gccgtgctgt tacgtcgctt
     7561 ctacccgcag gcgggcggtg cagacagcac gttgctgcgc accacgctga ttgtcggacc
     7621 tggcgcggtg atcctcggtc tgttgatcct cgtcttccac ctgacaagac cgtggacctt
     7681 ctggaagctg atgttccact acagttttac ctcggtgatg tcgatggggg tgatgctgtt
     7741 tcagctctac atggtggtgc tggtgctgtg gctggcgaaa atctttgaac atgatttgct
     7801 tgccctgcaa caacgctggt tgccgaagct ggggatcgtg caaaaggttc tgagcctgct
     7861 gacgcccgtt catcgcggac tggaaacgct gatgctggtg ttggcggtgc tgctgggggc
     7921 ttataccggc tttctgctgt cggcgctgaa atcgtatccg ttcctcaata acccgatcct
     7981 gccggtgctg ttcctcttct ccggcatctc gtccggtgtg gcggtagcgc tgatcgccat
     8041 ggcgatacgc caacgcaaaa atccgcattc cacggaagcg cactttgttc accgtatgga
     8101 aatccccgtg gtatggggtg aaatcttcct gctggtggcg ttttttgtcg gtctggcgct
     8161 gggcgatgac ggtaaagtgc gtgcgctggt ggcggcatta ggtggcggtt tctggacgtg
     8221 gtggttctgg cttggtgtcg ccgggctggg gctgattgtg ccaatgttgc tcaaaccgtg
     8281 ggtcaatcgc agttccggca ttcctgccgt gttggcggcg tgtggggcca gtctggtcgg
     8341 cgtgttgatg ctgcgctttt tcattctcta cgccggacag ttgacggtgg cgtaagccag
     8401 aaaagaggtg gtttctggac gtattccttc ctgaagtcgg ttttctggcg ttgttgttaa
     8461 gtctcggggt caacgtgttg accccgttga cggcctttgc gggagtgcgg ttgcgctggc
     8521 ctgccatgat gcgactcact tgcatcggca ttctggcgca gttcgcgctc ctgctgctcg
     8581 cctttggcgt actgacgtat tgttttctca tcagcgattt ctcggtcatt tatgtcgcgc
     8641 aacatagcta tagcctgctg tcgtgggaac tcaagctggc agcggtgtgg ggcggccatg
     8701 aaggttcgct gctgctttgg gtgctgctgc tttccgcctg gagcgcgctg tttgcctggc
     8761 attatcggca gcaaaccgat ccgctatttc cgctgacgtt agccgtttta tctctcatgc
     8821 tcgccgcact gctactgttt gtggtgctgt ggtccgatcc cttcgtgcgg atatttccac
     8881 cagcaatcga aggccgcgat ctcaatccga tgctgcaaca tcccggtctt atctttcatc
     8941 caccgctgct ttaccttggc tatggcggtt tgatggtagc ggcgagcgtg gcgctggcga
     9001 gtttactgcg cggcgagttt gatggtgcct gcgccagaat ttgttggcgc tgggcactac
     9061 ctggctggag cgcattaacg gcggggatca tcctcggttc ctggtgggcc tattgcgaac
     9121 tgggctgggg cggctggtgg ttctgggatc cggtggaaaa cgcctcttta ttaccctggc
     9181 tttctgccac tgcgctgctg cacagtttgt ccctgacacg ccagcgggag attttccgcc
     9241 actggtcgct gttgctggcg atagtaactc tgatgctgtc gctactgggt accttaattg
     9301 tccgttctgg cattctggtt tcggttcatg cgttcgcact ggataacgtc cgcgccgtgc
     9361 cgttgttcag cctgtttgca ctgattagcc ttgcgtctct ggctctgtat ggctggcgag
     9421 cgcgggacgg tggcccggtg gtgcgttttt cggggttatc gcgggaaatg ttaatcctcg
     9481 ctacgctgtt gctgttttgc gcagtgctac tgatcgtgct ggtgggaacg ctttatccga
     9541 tgatttacgg tctgctgggc tggggacgcc tctccgttgg cgcgccgtat tttaaccgcg
     9601 cgacgttacc gtttggcctg ttgatgctgg tggtgattgt gctggcgacg tttgtctctg
     9661 gcaaacgcgt gcagcttccg gcgctggtag ctcatgcggg cgtgctgtta tttgccgctg
     9721 ggatcgtggt ttccagcgtc agccgtcagg agatcagcct gaatttacag ccgggtcagc
     9781 aggtgacgct ggcaggatac accttccgtt ttgagcgcct cgatctgcaa gccaaaggca
     9841 attacaccag cgaaaaagcg atagtggcac tgtttgacca tcagcaacgc attggtgaac
     9901 tgatgccgga gcggcgtttt tacgaagcac gtcgtcagca aatgatggaa ccgtcaattc
     9961 gctggaacgg catccatgac tggtatgcgg tcatgggtga aaaaaccgga gcggatcgtt
    10021 acgcttttcg cttgtatgta caaagcggtg tgcgctggat ctggggggga ggattgttga
    10081 tgattgcggg cgcattatta agcggatggc gggggaggaa gcgcgatgaa taaaggactt
    10141 ctcacgctac tgctgttatt tacctgtttt gcgcgcgctc aggttgtaga cacctggcaa
    10201 ttcgccaatc cgcaacaaca gcaacaggcg ttaaatattg ccagccagtt acgttgtccg
    10261 cagtgccaga atcagaactt actggaatcc aatgcaccgg tggcggtcag tatgcgccat
    10321 caggtttaca gcatggtggc ggaggggaag aacgaagtcg aaatcattgg ctggatgacc
    10381 gaacgctacg gagattttgt tcgctataac ccaccgttaa cgggtcagac gctggtgtta
    10441 tgggcgctgc cagtggtgtt gttactgctg atggcactga tcctctggcg agtgagggcg
    10501 aagcggtgaa acagtcacaa ataccggtga aaatgctgac aacactcacg attttgatgg
    10561 tatttctctg tgtcggcagt tatctgttaa gcccaaaatg gcaggcggta cgtgcgcaat
    10621 atcagcgtca gcgcgatccg ctacatcagt ttgccagcca gcaaaccccg gaagcgcagc
    10681 ttcaggtatt gcaggataaa atccgcgcta atccacaaaa cagcgaacag tgggcgttac
    10741 tgggcgagta ctatctgtgg caaaacgatt acagtaattc gctgctggcg tatcgtcagg
    10801 cgttgcaact acgtggtgag aacgctgaac tgtatgcggc gctggcgacg gtgctttatt
    10861 accaggccag ccagcatatg accgcccaga ctcgcgcaat gatcgacaaa gccctcgcgc
    10921 tggactctaa cgaaatcacc gctctgatgc tgctggcttc cgatgcgttt atgcaggcga
    10981 attacgcgca agccatcgaa ctatggcaaa aagtgatgga tctcaactca ccccggatca
    11041 accggacaca gctggtcgag tcgattaata tggcgaaatt gttgcagcgg agatcggatt
    11101 aatcatctgg tgatgaagat tatgttattg agctgcataa aaaataatcg aatgaacata
    11161 tgccaaaaat aatcaccaat cagtattatt gcagattaaa caaataaaaa tctttccata
    11221 acaaatggtt attcattaat cctgccataa actcgcgtta ttatgcatta atgcagcgaa
    11281 aagctctgtt gttaaagggt tgcgcaacat accgcgcaaa tgatactgat cataagcgtt
    11341 aaaaaaatct acaaaccaac gcaacacaat tcatgccctg gcagtatgtc acgttctcgc
    11401 gtttctgaac ggggaacggc gctccattga ggaagttatt ctgatgaaaa atataaaatt
    11461 cagcctggcc tggcagattc tgtttgctat ggtgctgggc attctcctgg gaagctacct
    11521 gcactaccat agcgacagcc gcgactggct ggtcgtcaat ttgctctctc cggcgggtga
    11581 tatcttcatc catctgatta aaatgatagt tgtgccgatt gtgatctcca cgctggtggt
    11641 gggtatcgcg ggtgttggtg atgccaaaca gctcgggcgt attggcgcga aaaccattat
    11701 ctacttcgag gtgatcacca ccatcgctat cattttgggg atcactctgg cgaacgtctt
    11761 ccagcccggt gccggggtgg atatgtcgca gctggcgacc gtcgatatct cgaaatatca
    11821 gagcactacg gaagcggtac aaagcagttc ccacggcatt atgggcacga ttttgtcgct
    11881 ggtgccgacg aacattgtgg cgtcgatggc gaaaggcgaa atgctgccga tcatcttctt
    11941 ctcggtgctg tttggtctgg ggctttcctc cctgcccgcg acgcatcgtg aaccgctggt
    12001 gaccgtgttc cgctccatct ctgaaaccat gtttaaagtg actcacatgg tgatgcgtta
    12061 tgcaccggtg ggtgtgtttg cgctgattgc ggtgacggtg gctaactttg gtttctcttc
    12121 tctgtggcca ctggcgaaac tggtgctgct ggtgcatttc gccattctgt tcttcgcgct
    12181 ggtagtgctg ggaattgtgg cgcgcctgtg cgggttaagc gtctggatcc tgattcgtat
    12241 tctgaaagat gagctgattc tggcgtactc cactgccagc tctgaaagcg tactgccgcg
    12301 aattattgag aagatggaag cctacggagc accggcgtcg atcaccagct tcgtggtgcc
    12361 gaccggttac tcttttaacc ttgatggttc gacgctgtat caaagtattg ccgctatttt
    12421 catcgctcag ctgtatggca ttgacctgtc catctggcag gaaatcattc tggtgctgac
    12481 gctgatggtg acctcgaaag ggattgctgg cgtgccgggc gtgtcgtttg tggtgttgct
    12541 ggcaacgctg ggtagcgtag gtattccgct ggaaggcctg gcgtttattg ctggtgttga
    12601 ccgtatcctc gacatggcgc gtactgcgct gaacgtggtg ggtaatgcgc tggcggtgct
    12661 ggtgattgcc aagtgggaac acaaatttga ccgcaaaaag gcgctggctt atgagcgtga
    12721 agtgctgggc aaatttgata aaactgcgga tcaataattg aagattgccg gggttatcca
    12781 cccggcaatg tgtggatgcc tgatgcgacg ctggtgcgtc ttatcaggcc taccttaacc
    12841 gttggttaat ttttcaaact cttcacaccc ggtatcaaac ccttccatac agctcaggtt
    12901 caaccagtgc agcgcctttt gtttgttctt ctcgataaaa ccttcctcac cgtttaagaa
    12961 catcattccc gcccagtact cggaataacc ggttcgggaa attgcagagc tgcgtttgaa
    13021 ataccaggtt gccttgtcat cgtcggcctt aatgcccacg ccgttggcgt aaatcaaacc
    13081 aagcagcatt tgggcatcga cggcagagtc gttctccaga tcttccgagg cgttttctaa
    13141 cagcgaaatc gcctttggat aatccggttt acccgcttga gtatttacca gaatatgcgc
    13201 cagcgttact tcaccttctt tactgcccgc ttgcgccgct ttctctgcaa gaacttttgc
    13261 ttgtggatag tccagactga ccggattggt gattttgatc tgcgccagca gcgcgcaggc
    13321 atcagcatca ccgctgtcgg cggctttctg tgcccaatat tcggctttgc tcaaatcgcc
    13381 ggagctaaac cagctgtcgg caagaaaata ttgtgcgcgt cggtccccgg cctctgctgc
    13441 ctttaaatac tggctgccag gctcggagtc gttcgcgtgg gcaaagaatg tcaaaaacaa
    13501 catcaatgcg ataatttttt tcattttgat aatacgattg ggtagggagt aaccagtata
    13561 aagagttcga atggataaaa aaacagcctc cgaaaggagg ctgtagaaag gactgtatta
    13621 cgccagtgcc gcttcgcgca ggcgagtttt caacttgttg tactcgtcaa tcacgtactg
    13681 ctcggcggcg cgctgatcgg cgatcggctc gacgcgcacg gcgcagtatt tgtattccgg
    13741 cgttttcgta atcgggctta agttttcggt aaccagctcg ttacaggcac caatccacca
    13801 ctggtaggtc atgtaaatcg cccctttgtt cggacgatcg ctgacctgcg cacgggtgat
    13861 aattttgcct ttacgcgagt gcacccaaac caatgcctca tcttcaatac ccagacgttt
    13921 ggcgtcttcg gtattgattt gtgcgtagcc aggttcatca gccagcgccg ccagtgccgc
    13981 acagttaccg gtcatcgaac ggcaagagta gtgaccaact tcacgcaccg ttgacagtac
    14041 catcgggtac tcgtcggtga gtttgtcgat tggcgctacc cagtcgcagg tgaagaactg
    14101 cgccagaccg ttcggggtat caaacttctc tttaaacaga taagaagtcc cctgatcggc
    14161 atctgaagta tcgcggcaag gccactgaat gaagcccagt tcgcccattt tctcgtaagt
    14221 cgcaccgtag aaatccgggc acagatgacg caactcatcc cagatctcct gggtgttgtt
    14281 gtagtgcatc ggataaccca tacgggtggc gatttcactg atgatttgcc agtccgtttt
    14341 cagatcccat ttcggttcaa ctgctttgaa gaaacgctgg aagccacggt cagccgcagt
    14401 aaacacgcct tcatgctcgc cccacgacgt tgacggtaaa ataacatccg ccgccgacgc
    14461 ggttttggtc ataaagatgt cctgaacgat aaccagttcc agatcttcaa aggctttacg
    14521 tactgccgac agctccgcgt cagtttgcag cggatcttcg cccataatgt acgcggcacg
    14581 cacttcgcca tgcgctgcgc ggtgcggcag ctcgctgatg cgataaccgg tatgagcagg
    14641 caggctttcc acgccccagg ctttggcgaa tttctcgcgg ttagccggat ctttcacgta
    14701 ctggtatccc ggatacgtat ccggcagcgc gcccatatcg caggcaccct gcacgttgtt
    14761 ctgaccacga accgggttta cacccgcatg cggcttaccg aggttaccgg tcagcatcgc
    14821 gaggctggtc agagaacgca cggtttccac gccctggtag aactgggtta cacccatgcc
    14881 ccacaggatg gcggcgcttt tcgcctgggc atacatccgt gccgcctgac gaatctcact
    14941 ggcgctgacg ccggtgatat cttcaaccga ctccggcgtg tagccttcaa cgattttacg
    15001 atactcttca aagccttctg tacgggaagc gacgaacgct ttgtcgtaca gattttcttc
    15061 aataatgaca tggcccatcg cattcaacag cgcgatgttc gagccgtttt tcagtgcaat
    15121 gtgcatgtca gcaatgcgcg cggtttcaat tttgcgcgga tcgcagacga taattttcgc
    15181 cccgttacgt ttagcgttaa ttacgtgatt cgccacgatt gggtgggaat ccgccgggtt
    15241 gtacccgaaa acgaacacta aatcggtatt atcaatttcg ttaatagcat tgctcattgc
    15301 gccattaccg accgattggt gcagacctgc aaccgatggg ccgtgtcaga cacgagcgca
    15361 gcagtcaacg ttattggtac caataacggc gcgcgcaaat ttttgcatta catagttggt
    15421 ttcgttaccc gtaccacgcg aggagccggt cgtctggatg gcatccggac cgtacttctc
    15481 tttgatggcg ctcaggcgct cggcaacgta attcagtgcc tcatcccagg aaacaggttc
    15541 gagtttgccg ccacgctggc gacggatcat gggggttttc aggcgcgggg tcaggatctg
    15601 ggtatcgtta atgaaatccc agccataata acccttcaga cacagggtac cctggttggt
    15661 tttcccctgc gctgcctccg cccggacgat tttgccgtta tcgacgacca ggttgatttt
    15721 gcaacctgat gcgcaatagg ggcaaaccgt gacgactttt ttcatcggtc tcgctccagt
    15781 taatcaaatc acgcatacgc gctctcgact acagtatgca tcttttatgc cacattttat
    15841 ttggggtcat tccctgatat tacgggcact atttattcaa aactctgacg aaaaacaggc
    15901 tgtcgtcagt tttgacgtga cgaaacgaaa taccgcgtga cagccatcac gcggcagaca
    15961 ttttattttt tctcgacgac gggagccgcc tgatacccgc cgcccagcga tttcatcaac
    16021 tgaatgcttt ggatcacccg gcggctgtcc agcatcagta atgacatctc ttcggcaagt
    16081 actggcaacc gggcttcagt ggcctgtaag cggctggtta agccgcgctg ataggcggcc
    16141 tcggcagcgc gctgggtgaa gcgcgttgct tccacgcgtt cagcctgcat ttctcgctcg
    16201 tcgttgagcg tttgcagacg cgtgccgttg acggcaacgt cacgcaccgc gttcagtact
    16261 gactggttgt aacgttcaat catcatgttg ctggcggcgc gcgtgccttc gagattggca
    16321 ttcaaccgtc caccgtcaaa cagcggcaat ttcagacccg ggatgaagtt gaactggcga
    16381 ctggtttttt tgaataaggt atccaggtgg atggcatcaa ggccgaaaaa cgctttgata
    16441 tcaaagctcg ggtagaacag cgcccgcgcg gaatccacct gatctaatga cgcctgaaca
    16501 taccagcgca tggcttgcag atccgggcgt ctggcgagca actcataaga gagtgtcgcc
    16561 ggaatgccgg tctggactcg tggtaatgcc accggtttga tctccggcat atcgctggcc
    16621 cccgcgccaa tcaatgcacg cagagattct cgcgtttcgg tgatttgccc tttgacggca
    16681 gcaatttgtt tatcgaccgc cagaatctgt gcccgcgcgc cgtggaaagg cacttgtgct
    16741 tccagaccgt gcgccacttt actctggtgc gctttcaccg cgtaatcaat cacatcgcga
    16801 gtttgttcta acagatcgag catctgatag ctggcctgca tactgtaata aagctgcgct
    16861 acgcccgtgg tcagcgatag ctctactgct gcggtttctg ccagcgcggc attatgcgcg
    16921 ccaatggcgg cggcaaccgc tgagcgatgc acaccccaca aatcaagatc cagtccggca
    16981 aacaaaccta ctgtggcttc cgtatagtac ggcccgtcca tacccagtgc gggcgcatcc
    17041 atcgcataag ggcttaaaaa gccgttcgcc gagacgcgtt ggcggttgag catccctaac
    17101 gctgcgacct gtaattgtga accggcatct aacaaatcgg cctgggactg cgctttttct
    17161 tcccgcagtt tcgcttcggc gagggtgtgt gaaccactta gcgtccgttg gatcagcgca
    17221 tccagctgcg ggtcattgag ttgtttccac cactgcgcct gcggccagcc ggagctggca
    17281 agatgaatat cgtcggccag tttgatttgt tccggtttga gctgttgatg aggtgcagaa
    17341 tccttacgta ccagggcaca gccggaaatc agcgtcgtgc tgccgagaat gctgcacaac
    17401 agcagacgtg aaagttgacg attgatcatt gcgtggctcc ttgtgcctgt tccgtggcgg
    17461 gcgttaatgc cggggctgtc cagtctggca agcgggcgaa aagctggcag acgtgttgct
    17521 cctgttttaa taaggtgggc aggatggcct gcgaggcggg tgtctcttca agcgttattt
    17581 gaggagaacg gctgagtgcg gttgccagac cggcagcgta tttctccagg gcgtcggcaa
    17641 actgagctgc tctgtccggc tgtagtgcgt tatccagcgc ctgcgccgag ttccaggtgg
    17701 catcccaggc gtgaagaata tcgcggccct gacgaataac cgtttgcgaa cgttcaatca
    17761 gtaaggcgcg ttcttctctg tccagttgac gctccagcgc cacgcgttgg cacatctctt
    17821 cacaggcatt aaacgccgca tgcagaccga tacgaatttg cagataagtg cgcagagccg
    17881 tgacttcctg ctggcgtggg atccgcatta ctttacttaa catacccagc gtgccagcca
    17941 gtttttgcgg cagtgtgcgc gcttcacttt caggccagac aaaggtgtaa atcaccgcgg
    18001 acaccacggt accaatgatg atacccatgg cgcgatcgcg aacttccacc aggtcgtaca
    18061 ccgggccaaa aacgttttcg agcgtggcga gcgcgaaggt gaccaccatc tgtgtgccga
    18121 tataagaaga gcgttcagag ctggtggcaa tccatgcgcc caacaggaaa atcggtgcca
    18181 gcacaaacag caattcgaca atattgtcca gccagggcat gaccagtagc gtgaataaca
    18241 gcgccagaat cgcgccgcaa aaggccccgc caaaacgcag caccatcttc tggtacgacg
    18301 aaccgacatt tgggttagcg acgatcacgc atgtcagcat acaggtgtga atgccttccc
    18361 agtccacgcc gctgtagaag gtgtaacaga tcaaacaggc gagcagtgtt tttaccgcgt
    18421 agcgcatata gtctggattg gtaaaagcat cggcgaccat tgatggcggt ttggcggcgg
    18481 gcgttggcgg cgtattcggg tccatctgac ccagttgtaa caacgtctgg cagatattct
    18541 ccaggttaca ttcccgtgcc gccatcgctt cactttcagt gatccgccag tcgctttgcc
    18601 agcactgacc ttcagcaacg gcatgctgca gcttattgat ttctgaggct aatttttgcc
    18661 gaaattcaat aattgcctga gaatcagcaa aagaggtggg atcgtagcga ttcagcgtcg
    18721 agtaaatgta ggttaccgtt gccacgcagc tttgccacca tgcgctttga gttcgccagt
    18781 tggcatcgtc cgcgagacaa aagacattca gtttttgcag cgccagcgcc tctctttcaa
    18841 tccgcgtttc gggtagcggt gcgaggctgt ccgtcaggtg actaatggca tcatcaagcc
    18901 gatcattaag cgcttgatgc atttgcgtaa tggcacgact gggaaaccac agcacgccga
    18961 ttaacgtcat cagcaaggtt ggatagaggc caacaacgat acaccacagc gttaagcgca
    19021 cgaccacttc cggatagtcg agcatggcgg ggaaggtttg cccgtaaatg gcgacaatgg
    19081 cgacagcgaa aaagaccagt cccagccgat gggtgcgcat caaaaacata cagcccatca
    19141 ggatcggccc ggcgatgatc aatcggatca acggttcgcc gtatgaccat ttgtagatca
    19201 aaaacaggct gccgatctcc agcaccgtgg caaccacaaa caagatcgcg acaaatttgg
    19261 tgtaaaacgc gttcgactga ataccgtaaa acagcactgc cagcgataac gccacaaaag
    19321 ggatctcaaa agtcatcgag atcagaatca ccagcaggca gcctacccag agttgcacga
    19381 tctgcggcac gcggccgggt cgccgctcgc ttaactcttc atgaaagaac gccagcagcc
    19441 tgaccaccgg taatggcagg gagttgagcg cgctcattat tgcggctcaa gattagcgac
    19501 tgccgaagcg ccgatgcgga acatttccgg gtcaggttta tcgaccatga ttttgaccgg
    19561 aaaacgctgg gcaacgcgga cccagttaat agagcgagaa actttcggca ggccgcccag
    19621 caccaggccg ccgtcatccg gtagcacgcc gtagccaatc gaatccactt taccctcgaa
    19681 ggttttgccg ctgtcactca tcaggcgaat cgttgcgggt gtacctgagc gaatattttt
    19741 cagatcggtt tcgcggaagt tggcgatcac gtaccagtga cgagtgtcga ttagggtaaa
    19801 aatagggcgc atggcagaag caaattgccc gacggaggtt ttgagggaaa tgacccggcc
    19861 atcaaacggc gcgcgaacgg tcgccatttc cagatgaagt ttggtcaggg caatatccgc
    19921 ttcgaccgcc gcacgttgcg ccaccagcgc atcgacgccg ctgacggcgc tggcggctga
    19981 ctgcgcctgt aacaatacgg cattaagatc ggcttctgcg gcgcgctgcg cagttcttgc
    20041 acggtcaacg tcttccgctg agacaaaacc ctctttcagt aatggctcgg tgcggcgtaa
    20101 tgtatctgtg gcctgtttcg cggcggcacg ggctttttct accgtggcat taatcgagtc
    20161 ggcaccaaac tgttgcgcat caacgctacg ctgggtgagc ataatttgct tatccagcgc
    20221 cgcgagggag gcttcagctt tcgccagatt ggcttcgtac gggcgcgggt caatgcggaa
    20281 cagcaaatcg ccctgtttga ctgcctggtt gtcggtgacc gccagttcta caatgcggcc
    20341 gctgacttcc ggcaccacat caatggtatc tgctgacgcg taagcgtcat tagttgatgg
    20401 cgcactgtct acgcgccaga taacgaaaac aagggcaacc agcgccaatg caaccactaa
    20461 cagagcaggg aatttactgc gaggagcttt tttcggcgta ctttccatga gcgcgtctca
    20521 aatagattag aaaaatgcca gccagaacag catggcgtag agagcaaaaa gggcggtata
    20581 aataattccg acaaatgcca gattgatatt tgcccgctga ataactcgcc tcgtgatgag
    20641 catcaaaata aggctggcaa tggcgcagaa aaaccagctg ggataataag caccgatcac
    20701 cgggattgcc ggagataatg agcatcccga aaccatgaag acgggaatta tccaggctgt
    20761 ttttttgagt tgcatcgcca tgcgagagag aactgttggc atattaaata catcctgtct
    20821 gctgcgaaca atatctgtag tgaatatcaa tatccattga aggcgtcagt atggctattt
    20881 tctcgatgaa taaaaatagc tttattcagc caccgcattc ataacttata tattttattt
    20941 attaattggc tgtatatatt tttaaaataa atgcttttgg gggcttcagc gcccccaaaa
    21001 gcgagtgaca ttaatttggg gttacgatat tcacccacaa atcgaaatta tccagacagg
    21061 cgataatttc ttccagtttc gcgccattgc caataatttt ggctttcttc gcttttacca
    21121 gatccgccat tttggcttgc ccggtcagca cggcgtgcag atcttcacgg ctgatgtaga
    21181 aagaggcgtc agcttgggat tgcagtgttt tacggtagtt aagcacgcta tcgttcagcg
    21241 tcaggttgag gttatcgcca ttgctcatat tgaagttcag gctgatattt ttacccgcgg
    21301 ctttcgcgct atcgaggcga acggacataa agtcgaacag catttcgacc gacatcccgc
    21361 gaatggtgtc cggggaaccg gtggtgccgt ggctgaactt atgcacccct tcgcgcagct
    21421 ctttcgcgcc ggtcaggtag aaaccgcgcc aggtggcgga ctcggcctga tagcccagct
    21481 gttcaaagtt attcgcttgc agattcttcg cgacctggtc acccgggttg gcggcaatca
    21541 cctgtttcag cagttctgcc gaccagcggt aatcaccttg cttgttcgct tcttgcgcca
    21601 ggttgatgac acgggcagaa ccgcccagcg cctgcacgta acgtttaccc atctccacct
    21661 gaccatacgg atgcaggtta gccgggttac cgtcgtaata gccaagatag aagttataca
    21721 ccgcgcgggc gttgtggctg acagaaccgt aatagccacg gctggcccag ttattggcaa
    21781 gtgcaggcgg cagtttaatc atgtcgccga tttcattcat ggtgtagccc tggttcgcca
    21841 ggtgcagggt ctggtcgtga atgtacttga tagtatcgcg atatttacca atataatcat
    21901 tgatatgctt attgccccag accggccagg tgtgcggcat aaacaggact tccgcgtcgt
    21961 taccccacat atccagcgtt tcgttcagat actcggtcca cttgctggtg tcgcgggttt
    22021 tcgcgccgcg tagagtgtag aagttgtgca gggtatgcgt ggcgttctcg gcggtacaca
    22081 gcgctttcag ggccggaata tagaagtgca tttcggctgg tgcttcgcta cctggggtca
    22141 tcaggaagtc aaactccagg ccgtcgataa tcatcttctc accggttttg gtgatggttt
    22201 tgttcggtgc gatgatgctc gggtagcccg atgccagcgt tacgccaaga ccattgccga
    22261 tgttaccctg agggttatgc gccagcagca ggccgtagga gtaaagtgca cggcggctca
    22321 taatattacc cgccagtacg ttttcgctga tggcttcgtc cataaagcca gcaggggcga
    22381 taacctgcac tttaccggat ttcacatcgg cttcagagat aatgcctttc acgccgccat
    22441 agtggtcggt atggctgtgg gtataaataa ccgcaacaat cggtttttgc gggcgatttt
    22501 ggaagtaaag gtcaagggcg gcttttgcgc tcggtggcgt caccagcggg tcgatgacaa
    22561 taatcccggt atcaccttcg ataaaggtga tgttggagat atcctgaccg cgaacctggt
    22621 acattctgtc ggtgacttta aacaggccgg aaataccgtt taactgcgac tgacgccaca
    22681 ggctagggtt aacggtttcg ggcgcgggcg cattgatatc aaatttataa tcttccccgc
    22741 gataatatgg tttgccatta gcatcgcgca gaataccttc atccagcagc ggagcgataa
    22801 atccacgctg agcgtcgtca aaatcctgac gatcgctaaa cggtaatgat ttggcgtaat
    22861 tctgattaac ctgttgggta tattgagtgg cggctttcgc ttcatctgtt gcgtaagcgg
    22921 tatttaccat catgccgctg gcggcagtta acgcaataat aattttgctc aaacggaata
    22981 accgagagtt attcatataa atctccattt atcatttatg tcgtaaatat gtaatccatg
    23041 caaaatacgt catttaaagc tggggttatc ataattggga atttgcgact ttaataagtg
    23101 gaagtgtgag ctgaacgatc cattttatta ggcatttgtg ctgaagagga aaagttacag
    23161 taaaactatc attgcagaaa ataatatgag ccgttttatg tgtttattat atatattgta
    23221 cctgtcgttg gcacagcggg acattgcgct actgtgccaa cgctttactc atggggcttt
    23281 agcacaggac tgtggcaaaa aatgttgatg cgccaatatt gctgcaccct gagcgccatt
    23341 aaagtcagaa gatgaggcgg caataaagcg cacgacctga tgcggcagtg gacggcgcag
    23401 gtacttttgg gtcatggcaa tgagagtctc gcgtgggaag gcgggcatat ccatcacgcc
    23461 accgcccaga atcaccgcat cgggatcgaa cagattaatg ctggtggcaa tggcccgtgc
    23521 cgcgttttca agcagactct ggacgaaagg ggcgttttcc gcatggacga aaaggtcgct
    23581 caatgggtaa tttcggggct gttgttcgta ccagcgtctt agcgccattc cagagcaatt
    23641 ggtttccagg cacccaggat tgccacacgc gcagtgttgg gtcatatctc ccagggggat
    23701 atgacccagt tcgcctgcca caccgtgtgc acccgtccac ggcgcaccgt tcatccacac
    23761 tgcgaacccc atccccgtac cgagataggc cgccagaacc agttgttgcg taaggcggtt
    23821 ttctactacg tcccaggaga gttgcaggtt aacgtcgcgg gaaaactcaa ccggacaatt
    23881 cagcgtattt tcgagcttat cggcgagatc atataaatcc gccgctgtta acggcaggtt
    23941 aggcgtagaa attatggtgc gtttatcttt actgaccagc gccggaaatc ccatcaccag
    24001 accatgacag cgagcgttaa agcgcctgag ttgctcgtca atcatttcgc cgatacccga
    24061 caccaggccg ggagcaatga cttctgcggt ccgctttttt tcgcagtgta gcgtttcacc
    24121 ttctgctgtc cgcagacaaa agcggatatg cgttgccccc atatccacgc ccgctacgac
    24181 gttatgctgt ttttgcatga ggctgtacct cgctttttgc agccagaatc tgcgtggtca
    24241 taattctcca tgcttcgtcg atattttccg catgattaaa caggccggaa gtgccgacga
    24301 taaagacatc cgcccctgcc gccatcagtt tttcgtaagt tgccttgttg caggaaccgt
    24361 ccacctcaat ttcgtactcc agaccttctc gttcacgcca tgccttcagt tcggcaagtt
    24421 tatccagcat ttcaggaatg aacggttgtc cggcaaagcc gggatcgaca gtcatgaccg
    24481 taattttatc ggccttatgg atatagtatt tcatggcctc aactggtgtc tccgggttaa
    24541 ggatcagccc cactttcatg tcatgacagc ggatttcatc aatcaggcgg aacgcctgac
    24601 cgttgatagt ttccggatgc aaagtgatga aatcagctcc cgcacgcgcc agttgagcaa
    24661 tgtaatcctg cggccgcgtc accatcagat gacagtcgag cggtttagtt gccagttttt
    24721 taacctgact tacgaagaac ggtgagagtg tcagattggg gacaaagtga ccatccatga
    24781 tatcgatgtg aaagtaatcg gcatggctgt cgataaattc gatctgttct ttaaatttca
    24841 gcagatccat acacattaac gagggggaga ttttcatgtt caattcctta cttactgata
    24901 agacggtcaa gggcgacagc cgcgataatt aatccgccca tcaccaccag ttggtaatag
    24961 gtttgtacct gcaaaatatt cagaccgttg ttgatggtgc cgatgatcaa cccgccaatc
    25021 accacagaga aaatgcgccc cttgccgccg aagaaactgg tgccgccaat gatggcgctg
    25081 gcaatggcat aggtttcaaa acccataccg gcaagcggtt ctgcggcacc gagtcgtgca
    25141 gttgagacga cgcctgccag acctgcacaa acaccagaga tgataaacac caccaggatg
    25201 tggaatttca cgtcaatccc ggaatagaac gccgaatttt tgttgccgcc cagtgcgtag
    25261 atgttgcgcc cgagccgcat acgcgttgtc agaaaccaaa ggatgagcgc gacaattagc
    25321 gagaagataa cggggacagg tatcccaatt acgctggcgg caaagaagtt cacgaagtca
    25381 aaggagaagc cgtataccga gttggcatcg gagatcacca gcgtgatccc acggaaaatc
    25441 gcgttggtgc caagggtgat gatgaacggg tgtagccccg tccagttgac caggcagccg
    25501 ttgatcgccc ccagtgcgcc gccaaccagt acaccgccaa tcatcgctgc gagaaacggg
    25561 tcaacacctg ccaacatcag tttggcggtc accataccgg aaagcgccag aatcgcgcca
    25621 accgagaggt cgataccagc gaccaggata gcgaaaaact cgcccatgcc gatcaatacc
    25681 gtcacggagc tttgaacaaa aatctgggta atattattgg tggtcagaaa atattctggc
    25741 gacagcgaac caaaaatggc gacgatgatc gccaggataa aaaaggtgcc gtatttatcc
    25801 cagaacagcg caaagttgaa cggtttcttc tcgctcgctt cgctttttac tcttgtggta
    25861 aagcccatgc cataatctcc tcttcgctca tgtcatcgcg attcgtcagg atttgcgtca
    25921 gtcgtccttc gcagaacacg gcgatgcggt cgcagacggt gataatttca ggtagttcag
    25981 atgacaccat caggatgact tttccgtcgt ccgccagttg gcgcatcact ttgtaaattt
    26041 cggctttcgc gccaacgtcg atgccgcggg taggttcatc gaaaataatc acttccgggc
    26101 aacagcacag ccatttggag atcaggactt tctgctgatt tcccccggat agttcggtga
    26161 tattctggtt taccgaatga catttcagcg ccagcagttc gcgttgattt tcagcggtac
    26221 gttgctcgtc aacttcatga aacaagccca tcgcgccttt atagccgccg tctttcagac
    26281 tgcggctgat cgccatgttc tgagcgatgg aaaagttggg aaaaaaaccg ttatcccggc
    26341 ggctttcagt gatgtaagcc atcccttttt tcactgcatc caggggggaa cgtggagaga
    26401 tatctttgcc attaagacgg atttctccgc cagcgcgttt atcaacgcca aacagacaat
    26461 tcatcagttc agtacgtccg gaaccgacca gcccggcaaa gcctaatatt ctccccggca
    26521 gacgctaaat gagatatccc ggaccttttt tctgtcacgg ctggtgacgt tgcgcacctc
    26581 aaaaaccgta tcgtgcacaa ggttgctgac attctccttc atcgcgttaa aacggttttg
    26641 cagttcgcgg cctaccatca gacggacgat atcgtcattt gacacatcgc ttaccatgcc
    26701 gctgcaaacg ctgctgccgt ctttcatcac cgtatagcgg tcgcaaatac ggcgaatttc
    26761 cgccaactta tgcgagatat agacgatggc cgtaccctct ttacgcaact gattcatgat
    26821 cagaaacaga tagtccacct ctttattggt gagtgaggag gtgggttcat ccatgatgat
    26881 gactttggca tcgagcatca gcgttttggc aatttccagc atctgcttgt ggctgataga
    26941 tagattcgcc actttctcat ctaaatcaac tttcaagccc acgcgtaata acatcatggc
    27001 ggcgcggaca cgcatttctc gccagtcgat aatattgacg ccacagattt ttttcgtcag
    27061 atgacgacca atatataaat tttccagtac ggttaattca tcaataacgc tgagttcctg
    27121 ataaataatc ccgataccga gttgtgccgc taatttatga tccagcttgt tatagctaat
    27181 gttattaatg gtaatggtgc ctttggtcgg ctcatgtatt ccggataaaa ccttcattag
    27241 cgtggattta cccgcgccat tttctcctag taatgcatgt atttcaccag gataaaccgt
    27301 taaattaacc gactttaatg cgtgaaccgg accaaaggac ttgccgatcc ccgccatcga
    27361 tatatatggc gtggccataa ataacctctc tgcacgaaat atcgtattat tgcctgatgg
    27421 ctacatttat caggtctacg ttcaggttat tttgttggcc ggataagaca cttcattagc
    27481 atcatcatcc ggcatcattc agttttattg agtgaccagg attgaatcga ccagtttaaa
    27541 ttccggtgct ttatccagcg ggataacctt gccggatttc tcagcgttaa ccatcagctt
    27601 cagacccgtt gcgccaatat ctgccgggtt ctgcgcaacc gtcgcggtca tttgtccggc
    27661 ttccaccatt ttgcgggctt ccggaattcc atctgtaccg acgaccaata ttttgcccgt
    27721 ttttccggcg tttgcgactg cctgagcaac acccattgcc atcgtgtcat tcgcgcaata
    27781 gatcgcttta atattcggat tacgttgcaa cacgttagtg gcgacatcca gtgctttaat
    27841 gcggtcccag tcagcgggct ggctggcgac aagcttgatc tggcttgctt ttttgaaggc
    27901 ttcggtggca ccattacgac gcgcttcacc ggaggcgttg ccggctttac cctcaatgat
    27961 tgcgacttca ccaccttcag cgcccaattt gtcaataatg aacgacgcgc ctttcgcccc
    28021 gacagcaacg ttatcggtgg tgacaaaagc ttccacattg ccgccagctt ttttcagatt
    28081 atccatgtcg attttttcat cgagattaac cagataaatg ccttttttcc atgcgcgggc
    28141 gacaggcatg accagattca ctgaggataa tggagcgaag gcgatacctt tgtaattttt
    28201 attactgaga tcttcaaata actgcaattg agattgaaaa tcgccttctg aaggagaggc
    28261 aaaaatatca acgctgacgc ccagtgtttt tgcttcatct tcaatgcctt ttttcatatc
    28321 tacccaaaat gggttggaga gggttttcaa tacgacagca tattcggcgg cagcaaaagc
    28381 gctggttgac aacattaagc ccacgagtgt gccgctgaaa tatttcagat atttattcat
    28441 aataatgttc tcacggtaag gggacaggta actccgcgat ggcggaatta cctttttctt
    28501 atttcagcgc gcctggtgag aagaaatcaa caattgcgcc ggttttttgc atattaatat
    28561 tagcttgttc aatgttgagc tgggcgacag agacaaaaaa agcgtccagc aaagttagtt
    28621 gtaatattct tgccgaggca ttacgaccta ataacggcgt ttccggggct ggtgagcaaa
    28681 taatataatc ggccagtttc gctatcggtg aatggtagct atgggttata caaataatct
    28741 ttgccccgtt cttttttgcc agttctacgg ccgcttttac atcactggtt cgcccggaat
    28801 gggtcactac cagcacaaca tctccttcct gtaacaacga agcggacatc atcatgatgt
    28861 gagcatcagg ataggcctga cagcgtacgc caatgcgcaa gaacttgtgc tgtacatcag
    28921 cacagatagc atttgatcct ccggcaccgt acaaatcccg ctgtctggcc tgatagaaaa
    28981 agcgggcggc acggtggatc tcatcaacgt tgacgatcga ctgaccttcc ataatggtgc
    29041 gtaaagtgat gttaaatacc ttattcacca catcctgcgg cgcttcatca aaagccaact
    29101 cggaaggcaa tacctgttct gactgagaaa aataatcttc cagcgcactg cgtaagttac
    29161 gaaagccgct aaaccccagc agctttgata ccttaactat catcgcttca gataccgaca
    29221 gagcttctgc gacatcttta attgcgggtg cacaactcag gttaccgggt ttgagtaacc
    29281 actctacgat gcggctttca ttttctgtca ttccttcctg cttcattcgc aggtaaggcg
    29341 ctaaccctat accgttcgga agcgctgaat caaactctga ctggctcatc acgttctctt
    29401 gtccttgaat tgaagacgcc actatatcac cacagcaaga acagaaaaga tgctggcgac
    29461 ttc
//
LOCUS       DAZGCS010000065        28363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGCS010000065 DAZGCS010000000
VERSION     DAZGCS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.065"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..388
                     /locus_tag="ACHK01_002911"
     CDS             <1..388
                     /locus_tag="ACHK01_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097836.1"
                     /translation="KRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKNA
                     ILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDGK
                     IHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            453..1511
                     /locus_tag="ACHK01_002912"
     CDS             453..1511
                     /locus_tag="ACHK01_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8097837.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDVARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKK
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            1552..2274
                     /locus_tag="ACHK01_002913"
     CDS             1552..2274
                     /locus_tag="ACHK01_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO8097838.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            2271..3092
                     /locus_tag="ACHK01_002914"
     CDS             2271..3092
                     /locus_tag="ACHK01_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO8097839.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            3067..3324
                     /locus_tag="ACHK01_002915"
     CDS             3067..3324
                     /locus_tag="ACHK01_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO8097840.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            3336..3587
                     /locus_tag="ACHK01_002916"
     CDS             3336..3587
                     /locus_tag="ACHK01_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8097841.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3592..4173
                     /locus_tag="ACHK01_002917"
     CDS             3592..4173
                     /locus_tag="ACHK01_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097842.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            4170..5528
                     /locus_tag="ACHK01_002918"
     CDS             4170..5528
                     /locus_tag="ACHK01_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO8097843.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            5515..5868
                     /locus_tag="ACHK01_002919"
     CDS             5515..5868
                     /locus_tag="ACHK01_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO8097844.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5859..7535
                     /locus_tag="ACHK01_002920"
     CDS             5859..7535
                     /locus_tag="ACHK01_002920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8097845.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIEGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7539..7961
                     /locus_tag="ACHK01_002921"
     CDS             7539..7961
                     /locus_tag="ACHK01_002921"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO8097846.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7958..8563
                     /locus_tag="ACHK01_002922"
     CDS             7958..8563
                     /locus_tag="ACHK01_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO8097847.1"
                     /translation="MKFLPLLALLISPFVSALTRDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8532..10850
                     /locus_tag="ACHK01_002923"
     CDS             8532..10850
                     /locus_tag="ACHK01_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO8097848.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGHYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIASYYPCGIAWVDRKSSF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10847..11431
                     /locus_tag="ACHK01_002924"
     CDS             10847..11431
                     /locus_tag="ACHK01_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO8097849.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWEAQLPAGWTLRDNGDKRELRNTSGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            11433..12602
                     /locus_tag="ACHK01_002925"
     CDS             11433..12602
                     /locus_tag="ACHK01_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO8097850.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPNTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFSRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12599..13063
                     /locus_tag="ACHK01_002926"
     CDS             12599..13063
                     /locus_tag="ACHK01_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO8097851.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            13063..13794
                     /locus_tag="ACHK01_002927"
     CDS             13063..13794
                     /locus_tag="ACHK01_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO8097852.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13791..15020
                     /locus_tag="ACHK01_002928"
     CDS             13791..15020
                     /locus_tag="ACHK01_002928"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO8097853.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(15194..15463)
                     /locus_tag="ACHK01_002929"
                     /pseudo
     CDS             complement(15194..15463)
                     /locus_tag="ACHK01_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15860..16891)
                     /locus_tag="ACHK01_002930"
     CDS             complement(15860..16891)
                     /locus_tag="ACHK01_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8097854.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17162..17605
                     /locus_tag="ACHK01_002931"
     CDS             17162..17605
                     /locus_tag="ACHK01_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8097855.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17621..17908
                     /locus_tag="ACHK01_002932"
     CDS             17621..17908
                     /locus_tag="ACHK01_002932"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8097856.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17921..19177
                     /locus_tag="ACHK01_002933"
     CDS             17921..19177
                     /locus_tag="ACHK01_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8097857.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(19477..19677)
                     /locus_tag="ACHK01_002934"
                     /pseudo
     CDS             complement(19477..19677)
                     /locus_tag="ACHK01_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20099..21112)
                     /locus_tag="ACHK01_002935"
     CDS             complement(20099..21112)
                     /locus_tag="ACHK01_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO8097858.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SVEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21124..22440)
                     /gene="fucP"
                     /locus_tag="ACHK01_002936"
     CDS             complement(21124..22440)
                     /gene="fucP"
                     /locus_tag="ACHK01_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8097859.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22468..23388)
                     /gene="rbsK"
                     /locus_tag="ACHK01_002937"
     CDS             complement(22468..23388)
                     /gene="rbsK"
                     /locus_tag="ACHK01_002937"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8097860.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            23691..24473
                     /locus_tag="ACHK01_002938"
     CDS             23691..24473
                     /locus_tag="ACHK01_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO8097861.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(24475..24701)
                     /locus_tag="ACHK01_002939"
                     /pseudo
     CDS             complement(24475..24701)
                     /locus_tag="ACHK01_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(25464..25898)
                     /locus_tag="ACHK01_002940"
     CDS             complement(25464..25898)
                     /locus_tag="ACHK01_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO8097862.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRRDVLCCVSCGCPLTLQR
                     GNDGQQPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVLDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVLSEDARADNPLQRL"
     gene            complement(25886..26287)
                     /locus_tag="ACHK01_002941"
     CDS             complement(25886..26287)
                     /locus_tag="ACHK01_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8097863.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            complement(26454..27023)
                     /locus_tag="ACHK01_002942"
     CDS             complement(26454..27023)
                     /locus_tag="ACHK01_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8097864.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            27228..27428
                     /locus_tag="ACHK01_002943"
     CDS             27228..27428
                     /locus_tag="ACHK01_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8097865.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            complement(27843..27992)
                     /locus_tag="ACHK01_002944"
     CDS             complement(27843..27992)
                     /locus_tag="ACHK01_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO8097866.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT         6917 a         7283 c         7087 g         7076 t
ORIGIN      
        1 gaaacgttat ataaaatggt ttgccatcac aatttttatc agtatgttga gtgcctgtgc
       61 ccgtacggcc ccagtgcaac agataagcac cactgtcagt gtgggtcata ctcaggagca
      121 ggttaaaaat gccattttga aagcaggtgc gcagcgcaag tggattatga cgcaagtgtc
      181 ccctggagtt attaaagctc gctatcaaac acgaaatcac gttgcagagg ttcgtattac
      241 atatacagct acctactata acatcaaata tgacagtagc ctgaatctgc aggcttctga
      301 tggaaaaatt cataaaaact ataaccgctg ggtgcgtaac ctagataaag atatacaggt
      361 taacttatct acaggagcaa cgttatagtg atgctgatat cgggctggaa tggcccgata
      421 gtatttaact ttctctataa gaatggttat atatgtacga acaggacacg ctcagcgcac
      481 tggatgccat caccgaagca cagcggattg cctttgctcc tatgcttttc caggcagctt
      541 tgtgcctgcg caatgcagga atattagatt atttggatca acagggtaaa cagggagctc
      601 cccttaatgc gattactgag cacacagtcc tgaatgaata tgccgtgggt gtgttactgg
      661 atatgggctt aagcgggcga attattacct gtaaagaggg aatttattac ctggcgaaaa
      721 tcggtcatta cctcctgcat gacactatga ctcgcgtgaa tatggatttc acccaggatg
      781 tctgttacca ggggctcttc ttccttgccg attcactcaa tgaaggaaaa ccttccgggt
      841 taaaagtatt tggcgactgg ccgacgatat atccggcact ttcgcaatta ccagacgtcg
      901 cacgtgatag ctggtttgcg tttgatcatt actattcaga tggcgcattt aacgccgcac
      961 tgccttatgt attcgccaac aaccccacaa ccttgtacga tgtgggtggc aatacgggta
     1021 agtgggcttt gcgctgctgc aaattcaatg aaaacatcgc cgttacgtta ttagatctgc
     1081 cacaacaaat cgtactggcg aaaaaaaata tcgcgaatgc aggtttttct gatcgcatag
     1141 atttccatgc ggtggatatg ctaagcgatg ccccattacc aggcgaggct gatatctggt
     1201 ggatgagcca atttttagac tgtttttcac ccgaacaaat aattagcata ctcagcaaaa
     1261 ttgccagcgt aatgaagcct ggcgcaaaac tttgcatcat ggagttgttc tgggatgcac
     1321 agcggtttga agcgacatcg tttagcctga atgcatcctc actttatttt acctgcatgg
     1381 caaacggtaa cagccgtttc tatagtgcag agaaatttta cgactatctg aataaagcag
     1441 gattccaggt agcagagcgt cacgataatt taggcgttgg gcatacactt ttgatatgcc
     1501 aaaagaaata atcgtcactt aataattttg gtttaccgcg ggcgcgcgtt gatgaaattt
     1561 gcattaaata ttaccaactg gcaggcactg gcgcctggac ttagcgatgt gcagcaatgg
     1621 caagcatggt cgcgccagcc gtgggcgatt gaccctgccg cgccgttggc aaaattaagt
     1681 gaactgccaa tgatgaccgc tcgtcgtctc agttcaggca gcaaactggc tgttgaatgc
     1741 ggtctgacaa tgctacgccg ccatcagcca gacgctgttt tgtataccag tcgtcatggc
     1801 gaactggagc gcaattaccg cattgttcat gcactggcaa ccgaacaggc gctttctccg
     1861 acggacttcg cgctttccgt acacaactct tccgtgggta acctgaccat tgtggccaaa
     1921 cagccgatcg tttcgtcatc gctttcggct ggtcgcgaca gtttccagca aggtttgtgt
     1981 gaagtgctta gcctgctaca agcgggatac cagcgcgtgt tgatggtcga ttttgatggc
     2041 tttctacctg agttttatca cccgcaactg cccgcagaaa tgcctacctg gccgtacgcc
     2101 gtcgcgctgg tgattgaagc tggcgatgac tggcaatgtg aaacgcagcc agccattgcg
     2161 gtaaatgaaa caacactgcc gcaaagcatc ttgtttttac agcactattt acaaaatgca
     2221 gatgcttttt cactgcctgg cgagcgcgtg caatggcgct ggagccgcag atgaataagc
     2281 tgatgtcgcg cctgggatgg gcatggcgac tggtcatgac cgggttatgt tttgtcctgt
     2341 ttggtctcgg cggcctgctg ctttcagtcg tgtggtttaa catattgtta gttttggtgt
     2401 gggatacttc ccgccgccgt cgtttggcac gtcgcagcat tgccgccagt tttcgcctgt
     2461 ttttaactgt cgcgaaaggg cttggcgtgc tggattatcg tattgatggc gctgaaattt
     2521 tacgtcagga acgcggttgc ctcgtcgtag ccaatcatcc cacgctgatc gattacgtgc
     2581 tgctggcatc agtcatgcca gaaactgact gcctggtgaa aagcgcgctg ttaaaaaatc
     2641 cttttcttgg cggcgtcgtg cgagcggcag attatttaat caacagcgaa gcagaaaccc
     2701 tgttaccccg ctgccaacag cgactggcac agggtgacac tatcctgatt ttccccgaag
     2761 gcacacgaac gaagcccggt gaaaaaatga cgttacagcg cggtgcagcg aatattgccg
     2821 tgcgctgcac cagcgattta cgcatcgtga cgatcggctg tagcgagcat ctgctggata
     2881 aacaaagtaa gtggtatgac gtcccgcctg ccaggccgtt ttttacggtt gaagtccgtg
     2941 ggcgggtaga aatcaataaa ttttacgatg caacttcaca agagccgacg ctggctgcaa
     3001 gacagttaaa ccggcatctt ttgcttcaat tacagcaagg ttgtttacct ctatcaggaa
     3061 taaataatgc aagcgcttta tctggaaatt aaaaacctca tcatcagcac cctcaatctg
     3121 gacgacttga ctcctgatga tattgatatc aatgcgccac tgtttggcga cggtttaggg
     3181 ctggactcta tcgatgcact ggaactggga ctggcagtaa aaaatgagta cggcatcgtg
     3241 ctctctgcgg aaagcgaaga aatgcgtcag cactttttct ccgtcgccac tctggcctct
     3301 ttcattgctg cgcaacgtgc ctgaggaaaa ccatcatgac agaccaacaa accatttatc
     3361 aggaagtctc agccctgctg gttaagctgt ttgaaatcga tccgcaggac atcaaacctg
     3421 aagcgcgact gtacgaagat ctggaactgg acagcatcga cgccgttgac atgattgtgc
     3481 acctgcaaaa gaaaaccggt aagaaaatca agccggaaga gtttaaagca gtgcgtacag
     3541 tccaggatgt tgtcgaggct gtagaacgcc tgctacaaga aggctaattt gatgtcgggc
     3601 attcgctcgc taccgatgat taagttgctg accggattat tgctgctcgt ctggcctttt
     3661 ttaatctggc taggtttggt gcataacagc ctgcactggc tgttgccgct gatggcgctg
     3721 ttgcttttat tgcgcctgcg ccagccccgt cgccagacgg ggccgctcca ggtcgtcaca
     3781 aaaatcgttg ctgttgtggg cattgtattg tgtgtctcca gttttctgtt gaaaacgtac
     3841 cagttactgc tgttttaccc ggtagtggtt aacgcagtca tgctggcggt ttttggcggt
     3901 tcgctgcggt cgacaatgcc gatagttgag cggctggcac gtttgcagga gccagacctc
     3961 ccggaaaagg ccgtgcgata tacccgccgc gtaacgcagg tctggtgcct gttttttatt
     4021 ttcaatggca gtattgcgtt atttaccacc ttgtatggca acatgccctt gtggacagcc
     4081 tggaacggta tcattgctta ccttctgata ggtttcctga ctgcagggga atggctgata
     4141 cgtcgtaaga tgatcaaacg gaaaacacca tgaatcagac tctctcactt tcacagtggt
     4201 tgacagcctc tcgccctgta acaactcctg ttgcctggct tggggaatat acctggacac
     4261 tggggcatct gcggcatgat gtagcgcttt tgatcgacca tttacgcgac cagccaggca
     4321 atcgctgggc actttgtttt gaaaacagct atctttttat tgtagcgttg ttggccacgc
     4381 tacactcagg taaaatcccg gtaatcccgg gccataatcg cgccgctttg ttaaatgtac
     4441 aacgctcctt gtttgattgc gtcctgagtg ataaaaggct gggctggaac ggtccacagt
     4501 ttgttgtaag cacatctcac cagatgacga ccagcacgat agcctttgat gatattgctt
     4561 ctgacgcgtt tattgaactt ttcacatcgg gttcaaccgg acaaccaaaa caaattgcaa
     4621 aacctgttat cagccttgat caagaagcaa acatgctggc aactcacttt gcagaccgac
     4681 tactgggctg tcgttttgta gcatccgtga tgcctcagca cctgtatggg ctaactttcc
     4741 gtattttttt acccatggca cttggcctgc cattgcatgc cgcaatgctc tggtattctg
     4801 agcaacttgc cgcattaagc catacctacc gctatgcctt tgtcagcagc ccggcgttcc
     4861 ttaagcgact cgaccttcat ctgacgcctc ccccagtgga aatgattctc tctgccggcg
     4921 gtatgctacc atggcaggat gtagagaaaa cttccacctg gctgaatata tggcccgatg
     4981 aaatctatgg tagtaccgaa acaggtattc tcgcatggcg ttaccgtcaa caggataata
     5041 tcccctggtt tactttttct gatgttcatc tttcacagga aagtgagggg gttcgtgtct
     5101 tttcgccact aatccctgct gaaggattgg tgcttgacga tatgttgcaa ttcaatgaaa
     5161 acggtcagtt ccacctgatc ggacgacgtg gtcgagtggt aaaaatagag gagaagcgga
     5221 tatcactggt tgaaatcgaa caacgacttc tggcacttga cggtattatg gatgtcgctg
     5281 caataccatt aatacgcaat ggccgccagg ctatcggtgt cgtggttgta ccaaataaag
     5341 aggcccgtca aatatggcaa tgctccggag ggaaaactct ggagttgtcc tggcgcaaag
     5401 cactattacc atggcttgaa cctgttgcag tacctcgtta ctggcgcatt attgatgaaa
     5461 taccagtaaa cagtatgaac aagcgtgtct atgcgcaatt acaggagtta tttcatgata
     5521 cgccatgaaa ttgagcgcca tctggttcaa ccgcaggaga tggaaatcgt tctatacctt
     5581 gacccgatgc tctactggtt taatggtcat tttgccgtac agcctctgtt accaggcgtg
     5641 gcccagttgg actgggtaat gcattatgct accacgctgc ttgctccggg ctggcgtttt
     5701 cgtagtatcc agaatgttaa atttctggcg ccacttatac ctgagacgac tgttaccctg
     5761 caattaacat ggcaggaaac aagtcaggta ctcactttct gctatcagcg tcacgacggt
     5821 gacgcccgcc acaccgccag tagcgggaag atccgtctat gtcggtaaac ttttctccct
     5881 gcgtgttgat cccctgctac aaccacggcg cgatgatgcc gggcgtgctg gcgcgtctta
     5941 agccatttaa tctgccctgt attgtggtgg atgacggcag cgatgccgcc acgcaacagc
     6001 aactggataa tctggttagc gaacagcctg gcgtgacctt aattcgcctg gcagaaaacg
     6061 caggtaaagg cgcagcggtg atgcgtggct tgcaggcagc ggcagacgca gggttcagcc
     6121 atgcggtgca ggtggatgct gacggtcaac atgcgattga agatatcccc aaactgctgg
     6181 cactcgctga acaacaccct gtggcgctga tctccggtca gccaatttac gatgactcca
     6241 ttccccgctc acggctgtac gggcgctggg tcacccacgt ctgggtatgg atcgaaacgc
     6301 tctccctgca actgaaagac agcatgtgcg gttttcgcgt ttatccggtt gcgccgacgc
     6361 tgcaactggc aaaacgcgcc accatcggca agcggatgga tttcgacacc gaagtgatgg
     6421 tgcgcctcta ctggcaggga aataccagct atttcgtgcc gacccgcgtc acctatccac
     6481 tggacgggct ttcgcatttt gatgccctga aagataacgt ccgcatctcg ctcatgcaca
     6541 cgcgtctgtt tttcggcatg ttgccgcgta ttccttcact gctgatgtgc cactcttcca
     6601 gccactgggc gcggcagagt gaagttaaag ggttatgggg aatgcgcctg atgctgctgg
     6661 tctggcgtct gctgggaaga acggcgttta ccgcgctgct ttacccggtg gttggcgtct
     6721 actggctcac cgctgctcgt gcgcgtaaag cgtcgcaaga ctggctcgcc cgtgtgcgcc
     6781 aacaacaacc acaggcagca aaaatcaaca gctatcagca ctttctacgt ttcggcaacg
     6841 ccatgctcga caaaatcgcc agctggcgcg gcgagctgca actcgggcgt gatgtgctgt
     6901 ttgcgccagg cgcagaagcg gcgcttgacg tccgcgatcc gcgcggcaaa ttgctgctgg
     6961 cctcacatct tggcgatgtg gaagtgtgcc gggcgctggc aaaaattgag ggctacaaaa
     7021 ccattaacgc gctggtgttt agcgaaaacg cccaacgctt taaacagata atgcaggaga
     7081 tggctcccca ggcaggcatt aacctgatgc cggtaacaga tatcggcccg gaaaccgcca
     7141 tcctgctgaa agagaagctg gataacggcg aatgggtggc gattgtcggt gaccgcatcg
     7201 ccgtcacccc gcaacgcggc ggcgactggc gcgtctgctg gagtccgttt atgggccagc
     7261 ctgcgccctt cccacagggg ccgtttattc tcgcctctat tttgcgctgc ccggtgaatc
     7321 tgattttcgc cctacgccag cacggcaagc tgcatattca ctgcgaaacc tttgccgacc
     7381 cgctgctgtt gccgcgtggc gaacgccaac aggcgctgca aaacgctatc gaccactacg
     7441 ccgcgcgtct ggaacattac gcgctccagt cgcccctcga ctggtttaat tttttcgatt
     7501 tctggcaact gccggaaatt caggacaagg agtaaagggt gcttaacgat ccccgattta
     7561 ccgctgaagt cgagctgacc attccctttc acgacgtcga tatgatgggc gtggcctggc
     7621 acggtaacta ttttcgctac tttgaagtgg cccgcgaggc gctgctcaac cagttcaatt
     7681 acggctatcg gcagatgaaa gagtccggtt atttgtggcc agtggtcgat gcgcgggtga
     7741 aatatcgcca cgccctcacc tttgagcaac gcatccgcgt gcgtgcgcat atcgaagagt
     7801 tcgaaaaccg tctgcggatt ggctatcaga ttttcgatgc cgaaacgggc aaacgcgcca
     7861 ccaccggata caccattcag gtcgcagtcg acgagcaaag ccgcgaactg tgctttgtca
     7921 gccccgatat tctgtttgaa cgcatgggag tcaaaccatg aaatttttac cgctgctggc
     7981 gctgctgatt agcccgtttg tgagcgccct gacccgggac gatcttcagc aacgctttac
     8041 cgaacaaccg gtgatccgcg cccatttcga tcaaacccgg acgattaaag atctgccgca
     8101 gccgctgcga tctcagggtc agatgttgat cgcccgcgac caggggttat tgtgggatca
     8161 aacctcaccg ttccccatgc aactattact ggacgataaa cgcatggtgc aggtgatcaa
     8221 cggtcagccg ccgcaaatca tcacggcaga aaacaaccca cagatgttcc agtttaacca
     8281 cctgctgcgc gcgctgttcc aggccgatcg caaagtgctg gaacaaaact tccgcgtcga
     8341 atttgccgac aaaggcgaag gccgctggac gctgcgcctg acgccgacca ccacgccgct
     8401 ggacaaaatt ttcaacacca tcgatctcgc cgggcaaacc tatctggaga gcattcagct
     8461 taatgataaa cagggcgatc gcaccgatat cgctcttacc caacatcaac tgacaccagc
     8521 gcaactgacc gatgacgaac gccaacgctt tgccacccag taaacgcccc gcgctgttat
     8581 gggggctagt ctgcctggtc atggcggcgg cgttgctgat cctgctgcca caatcacgac
     8641 tgaacagtag cgtgctggct atgttaccca aacaggcgat gggcgatatt cctccggcgc
     8701 tgaatgacgg ctttatgcag cgtctggacc gccaactggt gtggctggtc agccccggta
     8761 aagaggctaa tcccagggtg gctcaggagt ggctgacgct gctgcaaaaa tccgctgcgc
     8821 tcggcgacat taaagggcca atggatgccg ccagccagca ggcgtgggga gcgtttttct
     8881 ggcagcaccg caacggcctg attgaccccg acacccgcgc ccgcctgcaa aatggcggcg
     8941 aagcgcaggc gaagtggatc ctctcccagc tttattctgc gttctccggg gtaagcggca
     9001 aggagctgca aaacgatccg ctgatgttaa tgcgcggctc gcagctggcg atggcgaaaa
     9061 atggccagcg tttacgacta atggacggct ggctggtgac gcaggatccc cagggccatt
     9121 actggtatct gctgcacggc gaactggcgg gatcgtcgtt tgatatgcag caaacccacc
     9181 agctcatcac gaccctgaat acgctggaaa aggctctgaa aacgcgttac ccgcaggcgc
     9241 agttgctctc acgcggcacg gtgttttaca gcgattacgc cagccaacag gcgaagcagg
     9301 atatctccac cctgggcgtg gcaacgctgc tgggggtgat attgctgatt gtggcggtgt
     9361 tccgctcttt gcgcccgttg ctgctttgcg tgatttccat cggcatcggc gcgctggcgg
     9421 gaacggccgc cacattgttg attttcggtg aattacacct gatgacgctg gtgatgagca
     9481 tgagcgttat cggcatttcc gctgactaca cgctctatta tctcaccgag cggatggtgc
     9541 acggcaacga cgtttcgccg tggcaaagcc tggcgaaagt acgcaatgcc ctgctgctgg
     9601 cgctgctcac aaccgtggcg gcatatctga ttatgatgct cgcccccttc cccggcattc
     9661 gccagatggc gatttttgcc gccgtcgggt tgagcgcctc ttgtctgacc gtcctgttct
     9721 ggcatccgtg gctgtgccgt ggtctgccgg tgcgcccggt tccggcgatg gcgctgatgc
     9781 tacgctggct ggcagcgtgg cggcgcaata aaaaactgtc gcggggtctg cccgttgcgc
     9841 tggcgctgtt ttcgctggcg gggatgtcaa tgctgcgcgt cgatgacgat atctcgcagt
     9901 tacaggcgct gccgcagcac attctggcgc aggagaaagc cattaccgcc ctgaccggac
     9961 aaagcgtcga tcaaaaatgg tttgtagttt acggcgactc cccacagcaa acattgcggc
    10021 gactggagaa atataccgcc tcacttgagt atgcgaaaaa agaggggctt atcagcaact
    10081 accgcactat tccgctgaac tccctggcgc ggcaggagga agacttagac ctgctgaaaa
    10141 cggctgcccc gacggtaaca aaagcactgc aaaatgcagg gctgacggcg gtgaaaccag
    10201 atctcaacgc catgccggtg aaggtcgatg aatggctggc aagccccgcc agtgaaggct
    10261 ggcgtctgct gtggctgacg ctggaaaacg gcgaaagcgg tgtactggtg ccggttgaag
    10321 gggttaaaag tagcgcattg ttgcaggaaa tcgccagcta ttacccttgc ggcattgcct
    10381 gggttgatcg caaaagctcc tttgatgaat tgttcgcact ttaccgctac gtcttaaccg
    10441 gcttgttgct ggtggcgctg gcagtgattg cctgcggcgc agtggcccgt ctcggctggc
    10501 gcaaagggct tatcagcctg gtgccttcgg tgctttcgct gggctgtggt ctggcggtgc
    10561 tggcgatgag cgggcaggcg gtgaatctct tttcgctgct ggcgctggtg ctggtgcttg
    10621 gcatcggtat caactacacg ctgtttttca gtaatccgcg cggtacaccg ttaacttcgc
    10681 tactggcgat cgcgctggca atgctcacca ccttgctgac gctgggaatg ctggtattca
    10741 gcgccaccca ggccatcagc agttttggca ttgtgctggt gagcggtatt ttcaccgcct
    10801 tcctgctttc gccgctggct atgcccgata aaaagagaac aaaaaaatga tcaaatccac
    10861 attctggcga gcgttcgccc tgaccgctac gcttatcctc accggctgta gccactcgca
    10921 accagaacag gaaggccgcc cgcaggcgtg gctgcaacct ggcacgcgca tcacgctgcc
    10981 ggcaccaggg atttcacccg cagtcaattc gcagcaactg ttgaccggca gcttcaacgg
    11041 caaaacccag tcgctgttag tgatgcttaa cgccgacgat cagaaaatca cccttgccgg
    11101 gctgtcgtcg gtcggcattc gcctgtttct ggtgacctac gatgcgcaag ggctacgcgc
    11161 cgagcaatcc atcgtcgtcc cacagttacc gcccgcaagt caggtactgg ctgacgtgat
    11221 gctcagccac tggccgatta gcgcctggga ggcgcaactt cccgcaggat ggacacttcg
    11281 cgacaacggt gacaaacgcg agctgcgtaa caccagcggc aaactggtca cggaaatcac
    11341 ctatctgaat cgccagggaa aacgcgtgcc aatcagaatt gagcagcatg tctttaaata
    11401 ccacatcacc attcaatact taggtgactg atatgattta tatttccgcc gtcggcatga
    11461 tcaacgccct gggaaataac cttgacgaaa tcgcagccaa cttggctcgc ggtgtcgccc
    11521 ccggcatgcg cccacgcgca ggctggttgc aggggcatcc gcaggcggtc ctggctggcg
    11581 tggatggcga gcttccgctt atcccggaaa aattcgctgc gcatcgctcg cgcaacaatc
    11641 agatcctgct ggcggcgctg gcgcagctcc agccgcaggt tgatgacgct atcgcgaaat
    11701 atggctgcga gcgcatcgcg attgtgcttg gcaccagcac ctccggcctg catgaaggcg
    11761 acacgcacgt taacctgcac acccacgggc agccgaacac cacgtggcac tatgcacaac
    11821 aagaactcgg cgatccgtcg cgttttctca gccactggct ggcgctcgac ggcccggctt
    11881 ataccctttc aaccgcctgc tcctccagtg ccagagcaat gatcagcggg cgcaggctta
    11941 tcgaagcggg gctggtcgat gccgccattg tgggtggcgc agacaccctg agccgaatgc
    12001 cgattaacgg ttttcacagc ctcgaatcgc tctcgccaac cttatgtcag ccgtttagtc
    12061 gtgaccgcgc aggtatcacc attggtgaag gggcggggct aatgctgctg acccgggaac
    12121 cgcagcccat cgcgttgctg ggcgttggtg aatcgagcga cgcttaccat atttcagccc
    12181 cgcatccgca gggcgaagga gcgatccgcg ctattaatca ggcgctgacc gatgcgcagc
    12241 ttacgccgga tgacgtcggc tatatcaacc tgcacggcac cgctacacag ctcaacgacc
    12301 agattgaatc aatggtggtt aacgccctgt ttggcgaacg cgtaccgtgc agctccacca
    12361 aacatttgac cggacacacg ctcggcgcgg ctggcattac cgaggcggca attagtatgt
    12421 tgattttaca gcgtgattta ccgctgcccg ctcaggactt cagcctgtcg ccgcgcgatc
    12481 ccacgctgcc accgtgtggc attatcgaaa aaccgcagcc gctggcacgt ccggtgatcc
    12541 tgtccaattc cttcgccttt ggcggcaata acgccagcat tctgctcggg agggtttcgt
    12601 gagccactat ttatcccccg gcgcttatct gccccacgac gcgcccatgt tactgctgga
    12661 agacgtggtg agcgtcagcg atgacagcgc ggtgtgccgc gtgacggttt cgcccagtgg
    12721 cgtactggca ccgtttctcg acccggacgg caatctccct ggctggtttg cccttgaact
    12781 gatggcgcaa accgtcggcg tctggtctgg ctggcatcgc caccagcagg gcaaaaacag
    12841 tattgagtta gggatgatac tgggcgcgcg tgaattgctt tgcaccactg gcattctgcc
    12901 cgcaggccag acgctgacca ttaccgtcaa actgctgatg caggacgatc gcttcggcag
    12961 ctttgagtgc agcatcaacg acggcgaggc gacgggccgc attaacacct tccagccgac
    13021 cgcggaagaa cttaccaccc tgtttcaaca aggagcgtcc tgatgagtcg ttcagttctg
    13081 gttactggtg ccagcaaagg cattggtcgc gccatcgcct gcaagcttgc ggcagacggc
    13141 tttaacattg gcgttcacta tcaccgtgac gccacaggcg ctcaggaaac gctgaatgcc
    13201 attgtcgcca acgggggtaa cgggcgtttg ctcagtttcg acgtcgcaaa ccgcgaacag
    13261 tgtcgggaag tgctggagca tgagatcgcg caacacggtg cctggtacgg cgtggtcagt
    13321 aacgccggga tcgcccgcga tgccgctttt ccggctttaa gcgatgacga ctgggatgcg
    13381 gtgatccaca ccaacctcga cagtttctac aacgtcattc agccgtgcat tatgccaatg
    13441 atcggcgcgc gccagggtgg gcgcatcatc acgctgtcct cggtttccgg cgtaatgggc
    13501 aatcgcggcc aggtgaatta cagcgcagcc aaagccggaa ttatcggcgc aacaaaagca
    13561 ctggctatcg aactcgcaaa gcgcaaaatc accgtcaatt gcatcgcgcc agggcttatt
    13621 gataccggga tgatcgaaat ggaagaaagc gctctgaaag aggcaatgtc gatgatcccc
    13681 atgaaacgta tgggccaggc ggaagaagtt gccgggcttg ccagttattt aatgtctgat
    13741 attgcgggtt acgtcacccg ccaggttatt tccatcaacg gagggatgtt atgacacgtc
    13801 gcgtagtgat tacaggaatg ggcggcgtga ctgcctttgg tgaaaactgg caggacgttt
    13861 ctgcacgctt actggcgtat gaaaatgcgg tgcgcaaaat gccggagtgg caggtctacg
    13921 atgggctgca caccctgctt ggcgcgcctg ttgatgattt cacgctaccg gagcactaca
    13981 cccgcaagcg tatccgtgcg atgggccgcg tgtcgcaaat gtcgacccgt gccagcgagc
    14041 tggcgttaga gcaggcaggg ttgattggcg atccgatatt aaccagcggc gaaacgggca
    14101 ttgcgtacgg ttcatcgacc ggcagtaccg gtccggtgag cgaatttgcc accatgctga
    14161 ctgaaaagca cacgaacaac atcactggca ccacctatgt gcagatgatg ccgcacacca
    14221 ccgctgttaa tactggcctc tttttcggcc tgcgcgggcg ggtgatccca acctccagcg
    14281 cctgtacctc cggcagccag gcgatcggtt atgcgtggga agccattcgc cacggttatc
    14341 aaacggtgat ggttgccggt ggtgccgaag agttatgccc ttcagaagca gcagtgtttg
    14401 ataccctttt tgccaccagc cagcacaatg atgcgccgaa aaccacacct tcaccgttcg
    14461 atgaaaaccg cgacggactg gtgattggtg aaggcgcagg tacgctcatt ctcgaagaac
    14521 tggagcatgc caaagcgcgc ggcgcgacca tttacggtga aatcgtcggc tttgcgacga
    14581 actgcgacgc ggcacatatt acccagccgc agcgcgaaac catgcagtat tgtatggagc
    14641 aatcgctgaa aatcgcaggg ttaagcgcac aagatatcgg ctatatttca gcgcatggta
    14701 cggcgaccga tcgcggtgat atggcagaaa gtctggcgac agcaaccatt tacggtgata
    14761 atgtgccgct ctcctcgctg aaaagttatt ttggtcatac gcttggtgcc tgtggcgcgc
    14821 tcgaagcctg gatgagtctg caaatgatgc gcgaaggttg gtttgcgcct acgctaaacc
    14881 tgaataaacc agacccgaat tgtggcgcgc tggattacat catgcatgaa gcccgcaagg
    14941 ttgattgtga gtttttgcag agtaacaact ttgcctttgg cggcattaat acatccatca
    15001 ttattaagcg ctggccctga gttatacaac cacatggaca tcccatgtgg tgattcttca
    15061 aaaaatctga atcaatgaat atgaacctca aattctctta cgcatactga agagcattcc
    15121 gtataacgat tcagcagaca aaactttatc agtccacacg aatcaacgga tgcacagatg
    15181 acacgcccgg ttatcaccgg gcaatggttg ccataacggg taacataagg gactgaacag
    15241 ttgtctttag cgcaacgaac tgatctaacg tacctgaacg gacaacctga aaacagcctt
    15301 ccatcctgca ttgtccgcca gtgcttatta ccaaccacgt ctgttgcagt aaagtaatca
    15361 gttgcgaaca ttatttatca atcgtgacgc tggctcgcgc atggcagaaa aggtcctgac
    15421 tgagcaacaa caggcatcgt actgttgctg ctccatgttg tttatatgaa cgcatcccgt
    15481 ttgcaaggct tccgtgtttt tgacgttgac ggtagattgc tgctctatat ccttattgca
    15541 gccggtgctg cgggcctcaa tgagattcgc tgactctcgc cttattcacg caacgagctt
    15601 taagctcaga aaaacatcca agtttggtga gtcacagtct tacctgtcgt tgtcatcaat
    15661 ataggaatgg ataatctgga gagcgatact gcaggctgat attgcggttt tgcgacaagt
    15721 ttttagcatc cgcttctacg ccaaatacct gaagtaggaa tggatgcgtt agcagcatac
    15781 atccggaact gcgcggggaa tagccggaaa cggctatccc taccacaata gctaactttg
    15841 taatagcgct cttttaggct catgtactct gacgtaattc tatctggtag ttgaattgca
    15901 cgatacgcga ctcttctgat tcatcatgat tattattcaa tagtccaatc agtaacttac
    15961 ctgtctctat ccccattttt ttgtagtcaa tacggatgga tgtcaaagtt gggtgtgttt
    16021 gatcgcaatt gtctgaaccg tcaagacaag ctacagcgat atttccaggg atttttaaca
    16081 aacgccgctg gcactcaaac attataccca gcgcaatctc ttcgtggctg caaatcacag
    16141 catccagctc tggttgtcgc agtaatatct cgtttaatgc gtaacgcccc aattgcagac
    16201 ttgcggcatc cggcatggtg ataacaagat cagtgttctg gtaatggctc agcattgctt
    16261 tatgccagcc gttcagttgc tgacgttgca ggcggttgtc catatgcgca ccgataaatc
    16321 cgatattctt ataaccctgc tccagcagat gttcggtcag tttatgtgat gcttcaaaaa
    16381 atgctgtttc gatggtaatt tgagccggac tgaaagggga accgataacg ttaatggtag
    16441 gtatatttgt tttctcgagt atctggtgag ttttatcagc caactgagaa ccaaaaataa
    16501 ctaacgccgc cgggttgcct tgaagaagcg tcatcagaat ttcagcttct ttgagctgat
    16561 tatactcatg acacccaagc agtagttgga attcattttt attaagcacc tgctgtaatg
    16621 actgcataaa gcgggagctg gctttatctg tcagggaagg aaccagtacg gcgactgtat
    16681 gactgtgacc agatgcaagg gcgccagcag ttctgttggg aatataaccc aactcttcta
    16741 cggcctgttc tatcttttcc ctcagcttgt cagaaacctg ttccggtgtt ctcagcgcgc
    16801 gggaaacggt catagttccg acacctgcaa agttagcgac ctcctgcagc gtcacccgtc
    16861 cggtaccacg gcgcttacgt gttttttcca tttcgactcc atggataatt acaaccagct
    16921 gttttcatta cttttatttc accattactc tttccagttt ttgcccacgg tagcaaatat
    16981 cctgttgcat tgtatatgca ggaaccgaat atagccaaag catggcggca gagaaatgat
    17041 agcgctatca caaagattgg tagcgttatc agatagcgct atcaaatgcg acaatcatca
    17101 cattaattaa tgcgtaaaat atctactctc ctgttaatta tcatcgggag ttatcagtag
    17161 catgttaaaa aatctattaa atacagaggt cgttcaggtc gtggaacagg tcaaagactg
    17221 gcgggaagcc gtggcgattt catgccgacc gttgattgaa aacggctcta tcgaaccgcg
    17281 ttatgttgat gcgatctatc actcgcacga cactatcggt ccgtactatg tggttggccc
    17341 tggtattgcg atgcctcatg cgcgcccgga agagggggct aacaagctgt cattagcact
    17401 gaccctgatc ccttcaggtg tgaattttga tgccgatgaa aacgatccag tgaaattact
    17461 gattgtgctg gctgcaaccg acagtaccag ccacattgaa gcgatttctc aactcgccaa
    17521 gttattcgat aacgaaaaag acatacaggc cattttgacg gccaaaacga cgcaggacat
    17581 tttgtctgtt attgcgcgct attaatccag aggaacattt atgaaaatta cagtggtttg
    17641 cggtaacggc ttaggcacca gcctgatgat ggaaatgagc attaaaagca ttttgaagga
    17701 tttgtccgtc agcgctgatg ttgatcacgt cgacctgggc tccgcgaaag ggacgccgag
    17761 tgatatctat gtcggcacca aagacattgc cgagcagtta gccgcgcagt ctgtcgcagg
    17821 caaaatcgtc gcactggaaa atatgattga taagaaagcc atgagagaac gtttgtccgt
    17881 ggtattaacc gaattaggcg cactgtaagc tgggggcgat atggatttct ttcgtttttt
    17941 gatgagcgat gtactttccg aacccgcagt gctggtcggt ttaatcgccc tgattggcct
    18001 gattgcacag aaaaaaccgg taaccgaatg cattaaaggt accgtcaaga ccattatggg
    18061 gtttgtgatt ttaggcgcag gggcgggttt agttgtgtcg tcactgggtg acttcgcaaa
    18121 cattttccag catgcctttg gtattcaggg tgtagtgccg aacaatgaag ccattgtttc
    18181 cgtagcgcag aaaagcttcg ggaaagaaat ggcgatgatt atgttctttg cgatggttat
    18241 caacattatg attgcccgtt tcacaccgtg gaaatttatc ttcctgaccg gtcatcacac
    18301 gttgtttatg tcgatgatgg tagcggtaat tctggcaaca gcaggcatga ccggcattac
    18361 gcttatagcc gttggctctt tggttgttgg cgtggcaatg gtctttttcc cggctattgc
    18421 gcatccgtac atgaagaaag tgaccggttc tgatgacgtt gccatcggcc acttctcaac
    18481 attatcttat gtattggccg gtttcatcgg cagcaagttt ggtaataaag agcactcgac
    18541 tgaagacatg aatgtgccga aaagtctgct gttcttgcgc gacacgccgg ttgctatctc
    18601 ttttaccatg agcataattt tcctggtgac gtgcctgttt gcgggtgcag atgcggtgaa
    18661 agagcttagc ggtggtaaaa actggttcat gttctctatc atgcaatcca ttaccttcgc
    18721 agctggcgtg tacatcattc tgcagggtgt gcgcatggtg attgcggaga ttgtcccggc
    18781 attcaaaggt atttcagaca agctggttcc gaatgccaga cctgctctcg attgcccggt
    18841 cgtcttccct tacgcaccta acgctgtact ggttggtttc ctgagtagct ttgctgcagg
    18901 tttaatcggt atgttcacgt tgtatctgct gaacatgatc gtgattattc ctggtgtggt
    18961 gccgcacttc ttcgtaggtg cagctgcagg ggtattcggt aatgcaacgg gtggacgtcg
    19021 tggtgcgatt ttgggtgcct tcgctcaggg cttgcttatt accttcctgc cagtattctt
    19081 attgccggtg ctgggtgata ttggttttgc taacaccacc ttcagtgatg ctgactttgg
    19141 cgcactgggt attttgttag ggattatcgt tcgctaatat tttgatgaca ttaatatcct
    19201 ccaggatttc aggaacgtgg aggatattaa ttcctcttat tttctccatt tttctttctt
    19261 ccagtcagga ctgcatccga ttaatcgtaa gcgctccgag aagtacgtag cgtaaggatt
    19321 attttacagc tgagaagttt cagtgcagca gttcatgcac acggttcgac ggctagtcat
    19381 tgatcttcat aattacctcg cgtaaaaaac ggggtaaggc cgctcatcca gcgtcatctc
    19441 cacaggtaat aattcaacgc caatgccaga agagttcgtt accggaagac gccgtgatat
    19501 acgccctttg atcagcgaga gcctgagcca tttgaacaga agattatcat tgatatcatg
    19561 ctcccgggca atacgggcaa cagaacccct tggttgtaaa gccagtttaa ccatttgaag
    19621 tttaaactca tttgaaaatg acctgcgggg ttcaactgat aatgtttttt gttccataac
    19681 aggtgtccac tagttgaaaa ggtgggtacc tatgttacca atactggctt aatggctaca
    19741 tacagcagtc ggtttacgca tacactttta tagtgcactc cggcctcatg aatataacgg
    19801 tggattaccg ccgaacgaat cggaaaatag atactggaaa aaaactgaaa ttgtgaccag
    19861 tttttgttga ccactacaaa taaataaaag agttgcagtg cttgctcggt aagaaaacaa
    19921 tggaggttaa gctcctcaaa gaagctgtgg agtacaccac tctacagtag ttgcgcaaat
    19981 ttggagtgga cgataatgtg atcgtctata agggcaacgc tatcatagtc ttgtcctggc
    20041 gggtaaaaaa acgcgcttac cttaacgata agcgcgccgc tgttcaggcc ttgagtggtt
    20101 attcaattcc tgtggtgact gtaaaagtgc gcgtttgctg cggtgcaacc tgaatcagcg
    20161 tgccattacg ttgcgcggca agatacccct caggccgaca ggttgcaggt aatgcaaagg
    20221 cggctacctg ttgctctccg ttataaagga tccagcgtgt cacataattt agttcagcac
    20281 tgtagaaacg agtaacaaac gtagtgccat cgggagagat catgcgaaac tctggctgat
    20341 ctgtataagc gtccagtttg tctgcaaaga agacaatttc tggatcataa aattccggtt
    20401 gactcagcgt cgacagagag gcttctccct gcataatccg ttgattaaac gccagccact
    20461 gagcggtggg attaacatgc gaaggcactg attcacgcaa tcttaatatt tcgtccggga
    20521 tattctggct gaatgtagca tttggtatat atgcataatt catgtggcac atatattgta
    20581 gtggcatatc tacagaagcc agattggtta cggccatctt aatatcgaac agtgtagagg
    20641 atttgtgaag gaccactgtt ggctgagcca gataatgatg accgaaaccc attacatact
    20701 cgtaacgccc gttaaggcgt aacatatctc cgtctaattc cagccatgct tcatccatcg
    20761 cggcacaggc catttcaccg tgtagcagat gagtatcttc cacagatggg cagccattag
    20821 ccagcaaacc tgaatgaaaa gcaaaacagc cataggtctc tatcacctct gtcgccggtt
    20881 taggctggcg aaacatattg cacatggtga ggccgtgtcc atcaaattgc gcatcccaaa
    20941 tcatctgccc catccaggga agaataatca aatgtccacg actgtttgca attttaagcc
    21001 cctcgacacc gctgtcatat cgaaaagacg tgacagtaaa atcactattt tccagcaaga
    21061 tacgaggttt ctcgccaaaa agcgcccgcc acaaattaat acgcgtactc ataacgattc
    21121 tcctcaggac gctgtgactt cagccagtgc ggtacgtact ttgctttcac gccagaagta
    21181 gactccgaca tagacaaagc agagcataga aaccaggaat gaaagctgta gtgagtggaa
    21241 catatctgca atatatccct gaattgccgg aaccaccgcg gcaccgacaa tagccataac
    21301 aatgactgct cctgccattt ctgtatgttc gttatcaact gtatccagtg ttcctgcata
    21361 gatcgtcgcc cagcaagggc caaacaaaac acttaccagg acggcgacat agaccgcgct
    21421 gaaacttgga gccagtgcaa catatgccag gaacagcgcc cctataacgg aatagagaat
    21481 caatactttt tccggattaa aacgcgtcat aaggatattg gctataaact tgccaataaa
    21541 gaagcaggca aagctataga ccatgaagtt tgaagcatca cgttcgttga tatcgcccaa
    21601 ctccagcgcc agacggatgg taaatgacca tactgcgacc tgcataccca cataaaggaa
    21661 ctgcgccaca ataccgcgac gaaagcgcgg atttctagcc agatagcgca gcgtatccat
    21721 tgctgacggg cgtttatggt gacttgtctg tgccacttta caggttggga agcgggttaa
    21781 aaggaacaac accatgacca caaccagaat cataatcata tacttatacg gttcaagggt
    21841 gttctctaac atcagcacct taaagttgtg aatttgctcg gcgttcattc ctgacatctg
    21901 cttttcaagg ctttccccct cggagaaaac cagatatttg cccaataaaa taccagacgc
    21961 agcaccaatc ggataaaagg tctggctgat attgagccgc aatgtggcat aggcttttgg
    22021 accgatcatt gaactgtatg tgttcgctgc agtttcaagg aaactcaggc caatcgcaat
    22081 cgcaaaaata gctgcaagga acatagtgta ggttgccata tgcgaggcag ggaaaaaaag
    22141 tgtacaacca ccaatataca gcgtcaggcc aattaaaatt gccaccttat aactggtctt
    22201 tttaatcaca agggatgctg gtattgcaat taaaaaataa cctccataaa atgcgctctg
    22261 caccaatgct gaagcaaagt tacttagcga aaatacactt ttgaattgag tgattaatat
    22321 gtcatttaat gcagctgcgc atccccatag cgggaataaa cacgataaca aaataaactg
    22381 gaacaaggga gtcttattca gatacccatc aggcatctga atgatgtttt tatcgttcat
    22441 agtgctacct ttaactgtgc aggatgatta ttcgtttaag gttaaaaatt cattaaattg
    22501 ttcaatactc ggataagatg attgcgtacc tttccctgtg acgctgaaag cggcaaagag
    22561 agcggctttt ttcaaagcgg cttcaacatc accgctttga acataataat gggaaaagca
    22621 accaataaat gcgtcaccag cgccactagt atcaacagca tttactttga atgcaggaac
    22681 atggacttcc tgatcgcggg tcatccataa tgcgcctttt tcgctcatgg taacaataat
    22741 attgttcagc cctttatcaa ctaacgaacg tgcggccaaa cgaatatgat cataagtatc
    22801 aaccgacata ccggttaata tttccagttc tgtttcattc gggataaaga aatcacattt
    22861 gcaggcataa gacatatcta actcacgcaa tgccggagcc ggatttaata acacttcaat
    22921 accatttttc ttaccaaact caatcgcgtg gtaaactgtt tccagttgaa cttccagttg
    22981 taaaacgatc aatttgcatt ttttcagatc ttctgcagct cgatcgatat cttccgggga
    23041 aagaaattta ttcgctccct taattattaa tatactattg ctcgagttgg cattaacaaa
    23101 gatcggtgca acaccactgc tggtacaggg gactttctca acataagtgg tattaattcc
    23161 ccatgattcg agattacgaa tagtattatc cgcaaaaata tcatcaccta ctttagtcag
    23221 catcaggact tttgaattca atttagccgc cgccaccgct tgattagcac ctttcccacc
    23281 acatccgatt ttgaaggcag gtgcttccag agtttctcct tctttaggca tctgattagt
    23341 gtaagtaatg agatccacca tattggaacc aataactgca atgtccattt cactacctct
    23401 tataaacttt cgcataacaa tggtatttaa ataacattaa catgttactt ttgcatcatt
    23461 tgtgactgag atcgcgatta gcacatcaac ccgatgttta tttaatagac ttccagtctt
    23521 atcactcagg ccaacactat ctaatcataa gcaacctaac aggattaata ccgaaaattc
    23581 agcagtctat acccttttca tttcaaaggg tcggtcgtat agtatggtaa ctaaaacaat
    23641 gtttactaat gccataatgt tatttttata acattttacg gagagagttg atggaaacga
    23701 agcaaaaaga gcgtatccga cgtttgattg aaatacttaa gaaaaccgac agaatccatt
    23761 tgaaagacgc ggcacgaatg ctggaagttt ctgtaatgac tattcgtcgc gatctccatc
    23821 aggaagatga acctctgcca ctgaccctac tgggtggcta tattgtaatg gtgcataaac
    23881 ccgcaccatc catgccagta atccaggacg ttccgagaaa tcatcgtgat gacttaccta
    23941 ttgcaattct ggccgccgga atggttaatg aaaatgatct gatcttcttt gataatggcc
    24001 aggagatacc gctcgttata agcatgatcc cggatgcaat caccttcact ggcatctgtt
    24061 actcacatcg tgtctttgtt gcgttgaatg aaaaacctaa tgtgacagca atactttgtg
    24121 gtggtacgta tcgtgccaga agtgatgctt tttacgatgc cagtaactct tcgccattag
    24181 actctctcaa tccgcgaaaa atatttattt ccgccagcgg tgtacatgat cactttggcg
    24241 tcagctggtt taatcccgaa gatcttgcca ctaagcgtaa agcgatggcc cgtggactaa
    24301 ggaaaatttt gctcgcccgc cacgccttgt tcgatgaagt agcctctgcc agcctcgcac
    24361 cgctctctgc atttgatgtt ctgattagcg agcgtccgtt accggcagat tatgttacgc
    24421 actgccggaa tgcttctgta aagatcatta cacctgattc agaagacgaa tgacttactg
    24481 aaaaaacacc gtactcttgt taaacatcgt cggattggac tgattacgtt gcactttcat
    24541 cacatattcc agtttatcaa tttggcttat catctactcc agacgctggt catcattgac
    24601 caatagtctg atatggcttt tgtcgctgtc ctgaatcggc aggcagaaaa tgccttcaac
    24661 gttaaaagca cgacgggaaa aagcccacaa aggttagtca ttacgcccgg ataataaaca
    24721 ggctcctccc tgttatggaa gccgttgtat tgtccataat gtctgggttt ttaatgcaca
    24781 ctggataaca accggacgaa tcttcaaata ttactggcgc tgacagccgg tttgaattca
    24841 tctcacaacc ctgcatagag cgaatctcct gcctgcacgt cactccactc catggtatca
    24901 acttcacact ctttatctgc ggctagtttc agtcaccaga taagcatcga ctatgaaagg
    24961 tgagccatga caacataacg ttggtaacgc tctgacgcct taacggaaga tgcctgccac
    25021 cacagggaat gtaaacgact gaagtgtggc ctttaatgcc gtgaacggct catggtctcc
    25081 tggcacggtt gccgccccaa cctgtaacaa cattccacag tacaatgtct gtcagagccg
    25141 gagcctccca tgcctgttga ataaaacccc ggatgcttcc cccgattctg caagtaatac
    25201 ttcggttatt tacactattt tatccctgtt gaatttggtt aagccggtgc agacgcgccg
    25261 ggaaacgcac gcctgaaccg gcacaataaa atcctgttac tggagacacg aatggaatga
    25321 cgaggcagcc tgtcagtcac aggggggctg ttggcataaa atcaacggaa aggccaccaa
    25381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa cggcatcacc
    25441 gcaaactgcg ccagaggcaa ccattacaac ctctgtaacg gattatcagc cctggcatct
    25501 tccgacagca cctcctgccg cccccacgcc ctgctgtata ccccggtacc acaacgcggg
    25561 cagcactttt ctccttcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
    25621 accgtataat ccagaaccgt cataccctgc agatgcaaac gacgggcctc tttctctgcc
    25681 gggtcaagcc aggtgcatcg cagcaggatt ttctcagcga cagtcatctg gtcatgttca
    25741 aaccacggtt gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
    25801 cagcacaaaa cgtcccggcg gttgtgcttc gctgcttctt tcgccgtaac aaaatgcccc
    25861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
    25921 gaatactgaa tcctccggtc tggcagtttg tgcagtactg ctccccataa taatcgtggt
    25981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
    26041 gctgcagacg cttaatcaag cgtacctccc tgcgttccgg tctgacataa gggcactcat
    26101 gaccgtgctt agtcagcccg tcgtcagtgt gttcaaacca gggacgttca gtgtcgtatt
    26161 gcgggtggta tctgagtgca ctgccgcaaa ggtggcaagt gtagcgatcg taaggtgcag
    26221 tctgtgcggt acgggcacca gtcagacgcc cgttgccatc aagtgcgaga aaggattttg
    26281 cgtacatcgt catattcctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
    26341 ggcagcgtgg tcaggtgtga atagcggttt tcagttcagt ctcagaaggg aaaatcagtg
    26401 tggtcgctgt gaacgcgtca tcacactaag gtcagataag tcgtctgtcg ggctcagcca
    26461 cgactgcagc caaagttgcg ctcaccggtg ccgcgagctt tggtatgctc cttcgccaga
    26521 tagccagcac gttccagcac ctgctgaaag ccagtgtcat catcgcgttc cagccacacc
    26581 gccggccagg ccggaaaatg caccagcgtg gcatggcacc cgacatccac tcccagggca
    26641 ctgcaccacg cctgtttaat cattccggcc agtgaccccg gagcgcggta atcgccggca
    26701 cgacaccagg tatcccggtt gaccagcagc agcaggtgat agtgtttttt gcctttaagc
    26761 tccccgaact cccgggccca ggcgtaatgc agggtcgagg aatgcacgcg tttaccttcc
    26821 cgatgcttac gtttctggta agcgtcgatt cgggctttca gggcattgat gaagcgggat
    26881 atcacagccg cgtccgttgc tgccggtaca tccgggagac gcagatcaac ccgcaatgcc
    26941 gtcagacggg gatgaacatc cagtgcgtgc cgcaccgtct cacgaatacg ttgctgccag
    27001 aaggggttgt atttgtaggt catggttaaa tctccgtatg gtttcgttca tacggattaa
    27061 ccacgccgta aaaaatgcgc agagtccctg aagtgatctc caaccaggca atatctcagg
    27121 cactctgaga taacccgaac ggtcgtctcc ggactgacgg ttgaattttc agcgttgttt
    27181 ttttaatgta aaaaaccggt tacgggtaag gctgtgagga gggagttatg gtgatacgca
    27241 aaaagaagtg ctgggactgt ggaaacgcga ttactcataa tactgtgtgt tgcccgtatt
    27301 gtggttctgt cgaccccttc ggctattacc ggaatacaga ccgtatcgtt accatactgc
    27361 tggcactgat tattgtggtc ctgctgacta ctgtcagcgt cagtgtgtat atactgtgta
    27421 gctggtgaga taacggttca tgtatcccct gagctgacag tgtaccggtt atattaagcg
    27481 taaccgacag gttctccttc ataccatatt aaatttttcc atgtattgcc gctgccttat
    27541 ttcgtcccat acaggaaaac tcgtctgagt ttctgacaaa tacgtgaaac tcacagcccc
    27601 tcaatatcaa ccacacgctg taagccggta atacctgaaa atcagcccat taccgcccac
    27661 gcctccggca ccattccctt ttgatttcga attctttgtt gtcgctatac cgcttaacgt
    27721 tcccggtatt atcctggttc ccgacaacag gaacatattc agcttttaat aacctgagaa
    27781 gagccaataa gaatcacaaa gcggataaca cctggcgcga tgcagttgtg attaccgaac
    27841 gctcagatga catatttcca gactgaagcg ggaacacgat cgtaaagctt gttcattgtc
    27901 agttctgcca gccagtgatc aaccacagag ttgaaggctt ccagttccgc agaggtaagt
    27961 ttataacgcg tgtgggaaat cactttttcc agcgtctccc gggatgaaca acgacggaac
    28021 tgctttggtt ttaacttcca ttagcctctc atcactttat gctgcaatta acgtgatact
    28081 gtcagcatac cgccagactg gcattctggt acccacccca aactcattac tcccgagcca
    28141 ccacttaacc ggtctctcag actgtcactc accaggtcgg atatgtttac cgctcagacg
    28201 gatgcttaat gcactctgtt cacgtacatt gcagatacgt tttatatacg cgggttatac
    28261 gccagctatc agggatagcg cagtctgttg ctactgcgtt ctcatcatgc ccactgatca
    28321 cactcaccgt gtttatttca catatatgaa ctcatgtatt tga
//
LOCUS       DAZGCS010000066        27951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGCS010000066 DAZGCS010000000
VERSION     DAZGCS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.066"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            342..956
                     /gene="evgA"
                     /locus_tag="ACHK01_002945"
     CDS             342..956
                     /gene="evgA"
                     /locus_tag="ACHK01_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO8097867.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            961..4554
                     /gene="evgS"
                     /locus_tag="ACHK01_002946"
     CDS             961..4554
                     /gene="evgS"
                     /locus_tag="ACHK01_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO8097868.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4610..5755)
                     /gene="yfdE"
                     /locus_tag="ACHK01_002947"
     CDS             complement(4610..5755)
                     /gene="yfdE"
                     /locus_tag="ACHK01_002947"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO8097869.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5829..6773)
                     /gene="yfdV"
                     /locus_tag="ACHK01_002948"
     CDS             complement(5829..6773)
                     /gene="yfdV"
                     /locus_tag="ACHK01_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO8097870.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6843..8537)
                     /gene="oxc"
                     /locus_tag="ACHK01_002949"
     CDS             complement(6843..8537)
                     /gene="oxc"
                     /locus_tag="ACHK01_002949"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO8097871.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8591..9841)
                     /gene="frc"
                     /locus_tag="ACHK01_002950"
     CDS             complement(8591..9841)
                     /gene="frc"
                     /locus_tag="ACHK01_002950"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO8097872.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10354..10986)
                     /gene="yfdX"
                     /locus_tag="ACHK01_002951"
     CDS             complement(10354..10986)
                     /gene="yfdX"
                     /locus_tag="ACHK01_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO8097873.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11282..11557
                     /gene="ypdI"
                     /locus_tag="ACHK01_002952"
     CDS             11282..11557
                     /gene="ypdI"
                     /locus_tag="ACHK01_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO8097874.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(11634..11876)
                     /gene="yfdY"
                     /locus_tag="ACHK01_002953"
     CDS             complement(11634..11876)
                     /gene="yfdY"
                     /locus_tag="ACHK01_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO8097875.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            12229..13149
                     /gene="lpxP"
                     /locus_tag="ACHK01_002954"
     CDS             12229..13149
                     /gene="lpxP"
                     /locus_tag="ACHK01_002954"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO8097876.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13505..13576
                     /gene="ypdK"
                     /locus_tag="ACHK01_002955"
     CDS             13505..13576
                     /gene="ypdK"
                     /locus_tag="ACHK01_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO8097877.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13641..14879)
                     /gene="alaC"
                     /locus_tag="ACHK01_002956"
     CDS             complement(13641..14879)
                     /gene="alaC"
                     /locus_tag="ACHK01_002956"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO8097878.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DDWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15256..16953
                     /gene="ypdA"
                     /locus_tag="ACHK01_002957"
     CDS             15256..16953
                     /gene="ypdA"
                     /locus_tag="ACHK01_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO8097879.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16968..17702
                     /gene="ypdB"
                     /locus_tag="ACHK01_002958"
     CDS             16968..17702
                     /gene="ypdB"
                     /locus_tag="ACHK01_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO8097880.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            17715..18572
                     /gene="ypdC"
                     /locus_tag="ACHK01_002959"
     CDS             17715..18572
                     /gene="ypdC"
                     /locus_tag="ACHK01_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8097881.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18575..21070)
                     /gene="ptsP"
                     /locus_tag="ACHK01_002960"
     CDS             complement(18575..21070)
                     /gene="ptsP"
                     /locus_tag="ACHK01_002960"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8097882.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDRDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(21095..22132)
                     /gene="ypdE"
                     /locus_tag="ACHK01_002961"
     CDS             complement(21095..22132)
                     /gene="ypdE"
                     /locus_tag="ACHK01_002961"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8097883.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(22132..23217)
                     /gene="ypdF"
                     /locus_tag="ACHK01_002962"
     CDS             complement(22132..23217)
                     /gene="ypdF"
                     /locus_tag="ACHK01_002962"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8097884.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(23232..24479)
                     /gene="fryC"
                     /locus_tag="ACHK01_002963"
     CDS             complement(23232..24479)
                     /gene="fryC"
                     /locus_tag="ACHK01_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8097885.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(24501..24827)
                     /gene="fryB"
                     /locus_tag="ACHK01_002964"
     CDS             complement(24501..24827)
                     /gene="fryB"
                     /locus_tag="ACHK01_002964"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8097886.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(25046..26011)
                     /gene="glk"
                     /locus_tag="ACHK01_002965"
     CDS             complement(25046..26011)
                     /gene="glk"
                     /locus_tag="ACHK01_002965"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO8097887.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            26215..27471
                     /gene="yfeO"
                     /locus_tag="ACHK01_002966"
     CDS             26215..27471
                     /gene="yfeO"
                     /locus_tag="ACHK01_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO8097888.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            27586..27912
                     /gene="ypeC"
                     /locus_tag="ACHK01_002967"
     CDS             27586..27912
                     /gene="ypeC"
                     /locus_tag="ACHK01_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO8097889.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
BASE COUNT         7338 a         6968 c         6507 g         7138 t
ORIGIN      
        1 gtgcatgtgt tgcacattat cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa
       61 aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcct gtaggattag
      121 tgagaagact tatagtgcta acttgaaact ataaatcatc ggtataatcc ctgattttat
      181 tgttgacatt tcatttatgc cgactattta tatggtatac ttgtcgaatt atcttaaagg
      241 aagctcagat tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact
      301 acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
      361 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
      421 tcttagcgga gttgactgaa gggggaagcg ccgttcagcg ggtggaaaca cttaagcctg
      481 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
      541 tgaggaagcg ccaatatagc ggaattatta ttattgtctc cgctaaaaat gaccattttt
      601 acgggaaaca ttgtgctgat gctggcgcta atggattcgt gagtaaaaaa gaaggcatga
      661 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
      721 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
      781 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa
      841 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag
      901 aatgtaaatc actgatggat ctttacacat tcgcgcaacg taacaaaatc ggctaatcac
      961 atgaagtttt taccctattt ttttcttctc tgttgtggtc tttggtcgac cataagtttc
     1021 gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
     1081 ccagtacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt
     1141 gcagtacaca agtcccaaac ggctaccttg ttgcataccg attcgcagca acaggttcgt
     1201 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
     1261 cgggaatacg ccgatcatca aaaagcaatg gacgcgcttg cagaaggtga agtcgatata
     1321 gtgttatcac atttagttac ttcgccgcct cttaatagtg acattgctgc aaccaaacca
     1381 ttgataatta cctttccggc gctggtaacc acccttcacg actcaatgcg accgcttacc
     1441 tcaccaaaac cagtaaatat tgctcgggta gcaaattacc ccccagacga ggtaattcat
     1501 caatcgtttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     1561 gtatcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     1621 tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat
     1681 aattttttct tgaccagaaa agaatccgtc attcttaatg aagtactcaa tagatttgtt
     1741 gatgctttaa caaatgaagt tcgctatgaa gtttcacaaa attggcttga tacaggaaac
     1801 ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcaa
     1861 catcccgatt taaaggtgct ggaaaatcct tactctcctc cctattctat gacggatgaa
     1921 aatggctcgg ttcggggcgt tatgggggat attcttaata ttattacctt gcaaacaggt
     1981 ttaaattttt ctccgatcac cgtttcacac aatatccatg ccggaacaca gcttaacccc
     2041 ggtggatggg atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttca
     2101 tttgcagaag ctttcataac aacgccatac gtttttgtca tgcaaaaagc acctgacagt
     2161 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg
     2221 caattaaaag agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca
     2281 tttcacaagg ttaaggaagg tgaacttgat gctctggtcg cgacacagtt aaattcgcgt
     2341 tacatgatcg atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg
     2401 aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
     2461 aaagcactga atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa
     2521 atgcccaatg tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg
     2581 acattatccg ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca
     2641 gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca
     2701 ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     2761 cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca
     2821 ttagaaaaca gtgactcacc ctttaaagat gttttttcta atgcgcatga agtcacagca
     2881 gagacgaaag aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag
     2941 aaaagatgca ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta
     3001 tatatttgtg gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta
     3061 gaaaaaaata aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt
     3121 cacgaaataa gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct
     3181 ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca
     3241 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
     3301 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt
     3361 gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac
     3421 ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact gagcaatgct
     3481 ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac
     3541 aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa
     3601 caacaacaac tctttaaacg ctacagccaa acaagtgcag gtcgccagca aacaggatct
     3661 ggcttaggct taatgatctg caaagaatta attaaaaata tgcagggcga tttgtcatta
     3721 gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag
     3781 caagtggcga ctgtcgaggc aaaagcagaa caccccatca cactacctga aaagttgagc
     3841 atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta
     3901 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
     3961 caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg
     4021 actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca
     4081 caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg
     4141 ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca
     4201 cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa
     4261 ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg
     4321 tttcaagcac tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac
     4381 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata
     4441 acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa
     4501 cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga ctaaatagcg
     4561 gctcccacaa tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
     4621 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
     4681 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa
     4741 tcaacatatt tctcgcctga gtttgtggca atttaatggc ctcagccaca cttaataacg
     4801 gcgcgacggg tacaccaact tcatgtattc tggctaacca aacttcagct gcctgcgttt
     4861 ttaacgtccg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc
     4921 tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa
     4981 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtattg aaaacatcaa
     5041 aaggtgccat gtagggatgg cgatttccca gacgttgtgg tgacttccca gttgcgatat
     5101 atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat
     5161 gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat
     5221 agacaccgcc gcatagatcc gcaagagagg taccaacgcg cactggcgga gcatcaggat
     5281 atcctgtttc catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat
     5341 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga
     5401 tttcttgaag cgtttcccat gaaaacccca gtttttccat tgtacctggg cgaaaattct
     5461 cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct
     5521 ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc
     5581 catccacata gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa
     5641 ttacccttgc gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg
     5701 tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct
     5761 tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac
     5821 atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag
     5881 cccagtacgc tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg
     5941 gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaga
     6001 tgttcgctgt tcaaatgaca tgccatacca acgagaagca gtgccagtgg catcagaatc
     6061 agcttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga
     6121 gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt
     6181 gggtcccatg ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt
     6241 gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta
     6301 ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta
     6361 acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga
     6421 tcgagaactg caaaccccag gaatccaatg gtaggtgaac ccgcaattaa tgcacataca
     6481 gctgcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa
     6541 catccgacaa taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc
     6601 ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt
     6661 ttattaaatg cgcgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc
     6721 aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta
     6781 tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt
     6841 atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct
     6901 gctgcagggt cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt
     6961 aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc
     7021 attaatttgt cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca
     7081 ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc
     7141 ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca
     7201 ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata
     7261 ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca
     7321 ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg
     7381 ttctcgcgca atacgtcgcg cacagcactt aatgcattaa agtaatttaa tggttgagta
     7441 tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata
     7501 tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc
     7561 atactggatg caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc
     7621 tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt
     7681 gccaataacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca
     7741 aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata
     7801 gacattggca ggaatggaat ctgagtactt tcaataaatt cacgaagctg ttcatcagct
     7861 tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc
     7921 gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact
     7981 ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc
     8041 aaatcaagat aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat
     8101 gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc
     8161 gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg
     8221 ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc
     8281 aaaccattga ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga
     8341 aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga
     8401 atgccttccg cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa
     8461 atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt
     8521 tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc
     8581 cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac
     8641 ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg
     8701 gcgtaaaggc agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt
     8761 gttccacttc aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca
     8821 tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt
     8881 tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc
     8941 gtgcatgggc tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac
     9001 aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt
     9061 cccagccttt acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc
     9121 ggggaactgc atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca
     9181 atttatcgag acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct
     9241 gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa
     9301 gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca
     9361 gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt
     9421 tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa
     9481 aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg
     9541 cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct
     9601 ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg
     9661 tgaagtaaag cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa
     9721 cgccgggacg ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag
     9781 atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca
     9841 tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta aggcgtaata
     9901 tcttctataa ataacccgca aaatatcgtc gtagctgcac tcgatatttt taaaatcctg
     9961 tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc ctttctgcat ggtaagtagt
    10021 gaggacgaaa agaagaatat gataaatatt gattcgtggt aatcagagag agtcgcgaca
    10081 tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta agggtatgat
    10141 tacgcttttt aaagcgtacc ataaaacata acataataaa tgatttgtga tgacacggga
    10201 ttattgggat ttactttgag gaagataaaa tgcgtgttta tagaatcgcg agcgccaaat
    10261 aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt
    10321 aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctgtc
    10381 gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt
    10441 atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata
    10501 ttggttttcc attacgccaa caccggccag gcgaagttct tccattgcgc cctttttatc
    10561 ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg gagttgctac
    10621 ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt
    10681 ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc
    10741 ggaagcttca ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca gagccaggcg
    10801 cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc
    10861 cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg gtgcattatc
    10921 agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg
    10981 tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc
    11041 atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac agttgtagtt
    11101 attatcgcaa gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg tcgcagagaa
    11161 ctattcttat gtaccacaaa gagatgtcat ttattatatc aatgtaaata ttgattaatt
    11221 atatgtaaat acagccataa catcgtaggt tctcagtgaa ccattttcag gataaatttc
    11281 tatgaaagta aacttaatac ttttcagctt atttttattg gtctctatta tggcatgcaa
    11341 tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag agactgaaaa
    11401 aacatcagcg atgacgacca cacactctac aaaacttcag ccatcacagg cgattttgtt
    11461 taagatgaga gaagatgcgc caccattaaa cctcacagaa gaaatcacgc ctacttatcc
    11521 gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg gcaggtgaac
    11581 aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac ccactatcgc
    11641 ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag aaacataaat
    11701 atccccgtca ctagttcatt accgccagtt aaccacatgg tgaagtaata aatgagtgca
    11761 gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag tacctgaccg
    11821 atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata aatcatagct
    11881 caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta agacaatcct
    11941 taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg caagactgtt
    12001 gtgtacggaa aaatatttac tttgcacgat taataatcag tagctgaaag cagtcagcga
    12061 gtgataaagt acagataccg cattcatgcc cctcgtggtt atgtcctgac tagtctttac
    12121 actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt accgctgtcg
    12181 acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat gtttccacaa
    12241 tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt tgggcttggt
    12301 gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg cacgcgtatt
    12361 ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa aaacctggaa
    12421 ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga aaactttcgt
    12481 tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga cagtcgcgta
    12541 cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat gcaaaatcgc
    12601 ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg cgtgatggga
    12661 ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat ggaatgggtg
    12721 cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc
    12781 attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca ggattatggt
    12841 cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac aaccaatggc
    12901 acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat ggtaagaaaa
    12961 gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta cccgacagat
    13021 gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagagatcat gcgcgcaccg
    13081 gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgttg
    13141 tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat aaccttatgg
    13201 tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta ttaccgtcag
    13261 aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgt
    13321 ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga
    13381 attctggggt gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca
    13441 actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg
    13501 taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt
    13561 tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt
    13621 tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt
    13681 agtaaaccgt cggcacggaa catcgcttta atcccacgaa tcgcctgacg aatacggtcg
    13741 cggttttcga tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc
    13801 ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg
    13861 gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg catttcaacc
    13921 atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt gtactgttcg
    13981 gcaatgtcgc gcacgcactg ttgatcgccc tccagcgccg caatcgccgc cacctgcaac
    14041 ggcgtaaagg tgccgtaatc gtgatagctt ttaatacgag ccagggcgct gaccagcgtt
    14101 ttgttgccaa ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta
    14161 aagaactcga ctgccacatc gcgtgcaccc ggtacctgca tgattgacgg cgctttccag
    14221 tcatcgtaga cgatatcggc ataggccagg tcgtggacca ccagcacatc gtagcgtttc
    14281 gccagcgcca caaccttttc gaagaactcc agctccacgc attgcgcggt tggattcgac
    14341 gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt
    14401 tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc
    14461 accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga
    14521 tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct
    14581 tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga
    14641 cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca
    14701 gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa
    14761 tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa
    14821 acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata
    14881 atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct
    14941 gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat
    15001 ggctttaaat gaggaactcc acttcaaaaa caggaaattt ttctacgcta acaagaaaat
    15061 taccaccaat tttattaaca ttataatttc aataaattta ccagccagaa gtgcattatc
    15121 aagagataca ttgctaatac accatttatc tttcaatccc cttttaaatt tatggttttt
    15181 cctcaaaaac ggcctgctgg tcatcagcct tcaggttacc tatcatagag gtttaatcct
    15241 tattcagagt cacccgtgca cgaaatattc aacatgctgc tggcggtctt cgatcgggca
    15301 gcgttaatgc ttatctgcct gttctttctc atccgtatcc gcctgtttcg cgaactgttg
    15361 cacaagtcgg cgcactcccc aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc
    15421 gccctgttca gcacctggtc cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt
    15481 atcgcggtga tgtccggcgg gattctgttt ggcccgtggg taggcatcat taccggcgtg
    15541 attgcgggta ttcaccggta tttaattgat atcggcggcg tgacggcgat cccctgcttt
    15601 atcaccagca ttctggcggg ttgtatatca ggctggataa acctgaaaat ccccaaagca
    15661 cagcgctggc gcgtcggtat tctcggcggc atgttgtgtg agacgttgac catgattctg
    15721 gtaattgtct gggcaccaac taccgcgttg gggatcgata tcgtctctaa aatcggcatt
    15781 ccaatgatcc tcggtagcgt ctgtatcggc tttattgtgc ttctggtgca aagcgttgag
    15841 ggcgaaaaag aggccagcgc cgcgcggcag gccaagctgg cgctggatat cgccaacaaa
    15901 acgctacctc tgtttcgcca tgtcaatagc gagtcattac gcaaagtttg tgaaattatt
    15961 cgcgatgaca ttcacgccga tgcggtggcg attaccaata ccgatcatgt gctggcctat
    16021 gttggcgtgg gtgaacataa ctatcagaat ggcgatgact tcattagccc gactacccgt
    16081 caggcgatga attacggaaa aatcatcatt aaaaacaatg atgaagccca ccgcacacca
    16141 gagattcatt ccatgctggt gatcccattg tgggagaaag gcgttgtgac cggaacgctg
    16201 aaaatttact actgccacgc gcatcagatc acctcgtcat tacaggaaat ggcggtcggt
    16261 ctgtcgcaaa tcatctccac ccaactggag gtttcacgcg ccgagcagct acgtgaaatg
    16321 gcaaataagg cagagcttcg cgccctgcaa agcaaaatta atccccattt cctgtttaac
    16381 gctttgaacg ctatttcatc atcaatccgt ctgaatccgg atactgctcg ccagctgatc
    16441 tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga gcaaatcgat
    16501 atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc ccgctttggc
    16561 gacaagctga cggttatcta tgatattgat gaagaggtga attgctgcat tcccagcctg
    16621 ctgatccagc cgttggtgga gaatgccatt gtccacggta ttcagccttg caaaggtaaa
    16681 ggcgttgtca ccatcagcgt tgcagagtgc ggcaatcggg tacgcattgc ggtgcgggat
    16741 accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc
    16801 aataaaattg gcctgctgaa tgttcatcat cgcgtgaagt tattgtatgg cgaggggctg
    16861 catatccgcc gcctggaacc aggaacggaa attgcgtttt acattcctaa ccaacgcacc
    16921 ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg aaagtcatca
    16981 ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa gagcacagcc
    17041 agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt ttgcagcata
    17101 accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggc gtgttgctgg
    17161 cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc gcgtggaaag
    17221 aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa ccgtatcagg
    17281 agtcacgtat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa cagcagacca
    17341 gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg gtgaaagatg
    17401 agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac gagaaaatga
    17461 cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc gaattttgca
    17521 gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc aatctgaaca
    17581 aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg aaagatctgg
    17641 attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta atgcatcttt
    17701 aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt ttcgccgatc
    17761 tgttcagcgg cctggtgctt aacccgcaac tactggggcg cgtctggttt gccagccagc
    17821 ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat atcgtgctgc
    17881 gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa ggagaaatgc
    17941 tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg atgctgttaa
    18001 gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc accacgtcgt
    18061 tgttgcatcc tgctcgccag atccagcttc ccagcctgca acgcggtgaa ggtgaagcga
    18121 tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc attcagccac
    18181 tggtgttaag tttgctgcat ctttgccgta acgtggtgaa tatgccgccg ggcaattcgc
    18241 agccgcgcgg cgattttctc tatcacagca tttgtaactg ggttcaggat aattatgccc
    18301 agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat catctgtcaa
    18361 aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg gtgcgaatgg
    18421 cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg gcacagcgtt
    18481 gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt ggtctgacgc
    18541 cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct gtttccagca
    18601 gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag tttttattca
    18661 ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg ttagcaccca
    18721 gcgtcagcat gatcaccagt tcgacttcgc ccatttctga ctgccagtca atcggtttcg
    18781 ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg tgcgggatcg
    18841 ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca tcttcttcca
    18901 gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg aactggatcg
    18961 cctgctcttt attgctaaaa tcccgatcaa caaagatatt ctccagcgcc agcagtgggc
    19021 gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc gcactgcggc
    19081 attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga cgaagctggc
    19141 ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc agcccaagca
    19201 gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc catttgcccc
    19261 gctgatgcgc agtggtaaca atctgctgca acatgcgcag gaacgatggc gtaatcgggt
    19321 tatacagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac tgggtcatat
    19381 cgttagaacc gatactgaag aaatcgacct catcgcagaa gtggtcgatg atgtaacaca
    19441 ccgacggcac ttccaccatg atacccagag taatcgtctc tgcatgacgc aggccatcgc
    19501 gcttaagctc aacgatcgct ttttggatct cgcccttcac ccataagatc tgatcgaggc
    19561 tgtgaaccat cgggatcatc aactgggcgt tgccaaaact ggcagcgcgc aaaatcgccc
    19621 gtagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg cgatagccga
    19681 ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg cctccgatgt
    19741 ccattgtgcg gaagataatc ggcttgtcgc ccgctgccag tagcacctgc tggtaggctt
    19801 caaattgctc ctgctcgtcc ggcgcgctgt cacgatccat atacagcatt tcggtacgga
    19861 acagcccgac gccttccgcg ccgttggcaa acgcgcctgg tgcttccaga gcggtgccaa
    19921 tattcgccgc gatatcaata cgcttgttat cacgggaata ggccagctgc gcggcagcct
    19981 gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc aacctgataa taaccgctca
    20041 ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcacc gccggttgcc
    20101 cggcataacg ggcaatcgca tccagcggca ggccgctcag taccgggatc gccgaggcgc
    20161 gggccagaat cagggtatgc gaggtgcgcc cggttttctc cagaatcatg cccgcaagat
    20221 ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc agaatggtcg
    20281 gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtgata tgcagcaact
    20341 gttcgctaat atcgcgaatg tcgctaacac gttcgcgcag ataatcgctg gcagaagcag
    20401 aaagcttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt ccctgatgct
    20461 gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc agcgacaaat
    20521 gggcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctgg ttcagttgct
    20581 cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcacactg gcggggattg
    20641 cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg ccgacgccgc
    20701 ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa cgcggcagcg
    20761 gatgcgccgt cagttctgcc tgcgtaggct ggacgctgtc gctgtcgata aagcgcacct
    20821 ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta atgttcaggc
    20881 tgcaactgtc attaaatagg gtgccggtgc caatcagcgc cagcgaactt ttcgcatcgg
    20941 cctttgcgtt ctggcgatgg ttaataaagg tgacttcact ttgccactgg ctgcactgtt
    21001 ctttaagttc ccacgccgga cgagcatgca gaccgttagg caaaggacag agaaattgaa
    21061 tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc agttgaacaa
    21121 ccgtctcacg ggtaagacgt tgaataaggg cagataaaag ttgctccatc tgcaaaatgt
    21181 cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc cccattacca
    21241 ctgtgggtac gccagtaccg gttaagtgca ccgctccgcc gtccgtgccg ccgttgctga
    21301 acatatctgc ctgcaacggt acgccaattt cagccgccac ggtttcgatc caggcggtaa
    21361 gttttggcgg cgcaatcagt gacttgtcgc ttaacaccag catcgggccg ttaccaatct
    21421 ggcgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta tccagcacaa
    21481 tggcgacgtc cggcgacacc gcgcgggtag cggtttgccc accgcgtaat cccacctctt
    21541 cgctggaact tgccaccagc cacacctccg caggcagttc ggcatcgtgc agctcacgta
    21601 gtaacgtcac cagcagatag caaccgaggc gatcatcaaa ggctttcccc atcacccgct
    21661 ggtgagggag aacctgaaaa gttgtatcaa aggtgacgcg atcgcctgga cgaattcccg
    21721 cctgcatcac ttcgtcatac gagcgcgcac caatgtccac gcgcatggcg ctgacgtcat
    21781 tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg gtggtgatgc
    21841 gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc acatcaattg
    21901 ccccttcgcg ggagatgcta cgcaccataa atcccacttc gtccatatgg gcgcagatca
    21961 tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga ccatcaaatc
    22021 gcacttcttt ttgcaggcga tccgcttctt ccagcaggat ctgccgcact tcctgttccg
    22081 aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc attatgcctc
    22141 tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt gcggggtaac
    22201 cagcacaaca tcttcgatgc gcacgccccc ttgccctggc aaataaatcc ccggctccac
    22261 ggtcagtaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac gcggatcttc
    22321 atgaacttca atgccgatag catgaccggt gttatgaccg aaatagtggc taaaacctgc
    22381 ctcggtaatc acttggcgcg cggcatcgtc aacctgctgg cagcgcacgc cggggcgaat
    22441 tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa acagcgggtg
    22501 agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat cagagcagta
    22561 gccctgatac agcgcaccaa aatcgagagt gacaaactcg cccgctgcaa caatcttgtc
    22621 gctggctttg ccgtgcggca gcgccccgcg ccagccgctg gcgacaatgg tgtcaaaaga
    22681 ggctttttct gcgccctgct ggcgcataaa ccactccagt tcagcggcta tctcgcgctc
    22741 gctcatcccc gcctgaataa agcggcgaat atgctccgct ccgcgatcgg caatcccaca
    22801 ggcgaggcgg attatctcca cctcctctgg cgttttgatt tgccgcagca catccggcgt
    22861 ggcgctaacc agtttcgcat tgagttcaga cttccagcgg tgcgcggttt cccaactcac
    22921 ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat
    22981 agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc
    23041 cacctcaacg taatagcgcg aatccaccag aatgtgcgca ctttcacggc taataaccac
    23101 atagcctgat ccggtggaga tccccagatg cggctgtttg ttctgccgtg aggagagaag
    23161 cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc gaagcgagta atgtcatctg
    23221 gattttcctt cttacaggct atcgattaac aatttgcctt tacggaacat catcagacgc
    23281 aggaacacca ccatcagcgc agttatgact gcccccagcg cgatccccgc catatagacg
    23341 cccaggttag tgaccagcgg ccatgcccag atagcggact ctgggaacca ttgcactgcg
    23401 cccagccaga cagcggcggt tgagccgaca atagcgccga ccatatacga cggaatggcg
    23461 gtgatggggc tttccagcgc aaacggaatc gcaccctcac tgatccccat aaacgccagg
    23521 aacatggcgg tcttaccctg cggataaagt tgagcgttga acaggcgttt gccggttaaa
    23581 cgacggtcga taatggtcgc cagccccaga ccaatcggcg gaataacgat agcgatagaa
    23641 cgcgcggtaa ccggtaatac gtggtcggtg gtaaagctga aggcaacgaa acctgccgct
    23701 ttgttgattg ggccaccaag gtcgattgcg gtcgccgcgg caatccccat tgcatacatc
    23761 agcgcgccct tctcacctgc ggcagtcagc acggtacgga taccgccgtt gatccagcca
    23821 ccaaatgggg tgatgacata gtacatcgcc agcatgacaa aaatggcgga aagaatcggt
    23881 agcaaaaatg tggttttgaa cgctaacagg aaatctggta actggatttt ctggttcatc
    23941 catttcacaa ggtaaccagc aacgatagat ataatcagcg caccaatgaa ggtggacggc
    24001 accggcgaag aggtcgccca ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc
    24061 tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc gccgatagag
    24121 ttcgccacaa aagcggcaaa catcggaata gcaaagccaa acaacacgcc gccgaaggac
    24181 tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac cggagaattt cccgctatta
    24241 agagcatcca tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg
    24301 ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac
    24361 atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact
    24421 gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata
    24481 acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg
    24541 atgatgccta cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat
    24601 tcgaaacgtt cgttatcttc cggggtaact gccacggaat ggatgatgat ggtcgcttcg
    24661 gcgatatcct gcgccgtcag gcgattctgg atgccgtccg cgccctgggt ttcaattttc
    24721 acttcataac cggcttctac cgccgcttct tccagcgcct gagcggccat aaaagtgtga
    24781 gccaggccca tcgggcaggc acataaggca atcagtttct tactcatcgt taattcctcg
    24841 ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca
    24901 aataatgcat ttactggaga attaatcccg ctgtatggaa gtgtgacgca ggtcggcctt
    24961 gtgaggggac acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt
    25021 tacctcccga tataacagga aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg
    25081 caccggaacc gagaagaccc ggattgtcat ggacgatgag atacaccgga atatcatgga
    25141 catattcttt aaagcgccct ttatcttcaa atgcggcacg gaaaccagag gctttgaaga
    25201 actcaaggaa gcgcggcacg ataccgcccg caataaacac gccgccaaat gtcccgagat
    25261 tgagcgccag attgccgcca aaacggccca taatgacgca aaacagcgac aacgcgcggc
    25321 ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt
    25381 ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa attcaccagc ccagggccag
    25441 aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg
    25501 cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc
    25561 aacgcttatc gacatggacc agatgcgcaa ccccaagccc tgttccggca ccgtaaaccg
    25621 caataggttt accttctacc ggttctgcgc caccaaactg aatcagatgc tcttttttca
    25681 gcatcgggat cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac
    25741 cgagattctt tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc
    25801 agtcaccggt aatcgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt
    25861 cttcaagata aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct
    25921 tagcctgcga gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc
    25981 ccacatcacc gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata
    26041 attctttctc acactgtaaa tacctggggg cataacaaca acgccggaaa ggcaggctcc
    26101 ctgtaaatat cgatctgggt cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat
    26161 gcctttttgc aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc
    26221 catccgcgag ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg
    26281 tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg
    26341 caacgactgc cgggaactct ggggatagcc caggattcac ctttctggat catcggtgta
    26401 ttaacgctaa caggtattgc ggtggggttg gttatccgct tcagccaggg tcatgccgga
    26461 ccagaccccg cctgtgaacc gctgatcggc gcaccggttc caccttccgc gctaccagga
    26521 cttatcgtag cattaattct cggtcttgct ggcggcgtca gcctggggcc agaacatccg
    26581 atcatgaccg tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc
    26641 aaccgaatgg agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca
    26701 cctgttgcgg cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta
    26761 tgggatcgtc tttttgcgcc attaatggcg gcagcagctg gtgcacttac taccggatta
    26821 tttttccatc cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat
    26881 attctcagcg gtgcaattgt cgcggcgatt gccatcgcag caggaatggt cgccgtatgg
    26941 tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa atccggtgct cgtgctgggt
    27001 attggcggat ttattctcgg tattctgggg gttattggtg gaccagtttc gctgtttaaa
    27061 gggctggatg agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt
    27121 ttgctggcgg taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt
    27181 gggcgaatct tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac
    27241 gtacccgccg taccagcggc aataaccgtt tcttgcgcta ttctcggcat cgtgctggtg
    27301 gtaacacgcg atggctggtt aagtcttttt atggcggcgg tcgttgtacc caataccaca
    27361 ttgctaccgc tgctctgtat cgtcatgctc ccggcatggc tgttattagc aggtaagccg
    27421 atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg ataacgttta gcaaaaaatg
    27481 ctctccttta tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt
    27541 gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg
    27601 tttctggcgg ccgccctgat ggcatttacc ccgcttgcag caaacgcagg tgaaatcacc
    27661 ctactgccat caatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac
    27721 ggtggtcact ggcgcgaccg tgactactgg catcgcaatt atgagtggcg taaaaaccgc
    27781 tggtggcgtc atgataatgg ctatcatcgt ggctgggata agcgtaaagc gtatgagcgt
    27841 ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatgga
    27901 caccgccatt aaacacattc gccaatggaa cacaatgcct gatgcgacgc t
//
LOCUS       DAZGCS010000067        27294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGCS010000067 DAZGCS010000000
VERSION     DAZGCS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.067"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            412..1551
                     /gene="wza"
                     /locus_tag="ACHK01_002968"
     CDS             412..1551
                     /gene="wza"
                     /locus_tag="ACHK01_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO8097890.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKIHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1557..2000
                     /gene="wzb"
                     /locus_tag="ACHK01_002969"
     CDS             1557..2000
                     /gene="wzb"
                     /locus_tag="ACHK01_002969"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO8097891.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2003..4165
                     /gene="wzc"
                     /locus_tag="ACHK01_002970"
     CDS             2003..4165
                     /gene="wzc"
                     /locus_tag="ACHK01_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO8097892.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4343..5182
                     /gene="wcaA"
                     /locus_tag="ACHK01_002971"
     CDS             4343..5182
                     /gene="wcaA"
                     /locus_tag="ACHK01_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO8097893.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5185..5673
                     /gene="wcaB"
                     /locus_tag="ACHK01_002972"
     CDS             5185..5673
                     /gene="wcaB"
                     /locus_tag="ACHK01_002972"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO8097894.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGDNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5670..6887
                     /gene="wcaC"
                     /locus_tag="ACHK01_002973"
     CDS             5670..6887
                     /gene="wcaC"
                     /locus_tag="ACHK01_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO8097895.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            6862..8079
                     /gene="wcaD"
                     /locus_tag="ACHK01_002974"
     CDS             6862..8079
                     /gene="wcaD"
                     /locus_tag="ACHK01_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO8097896.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWSAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8090..8836
                     /gene="wcaE"
                     /locus_tag="ACHK01_002975"
     CDS             8090..8836
                     /gene="wcaE"
                     /locus_tag="ACHK01_002975"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO8097897.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8852..9400
                     /gene="wcaF"
                     /locus_tag="ACHK01_002976"
     CDS             8852..9400
                     /gene="wcaF"
                     /locus_tag="ACHK01_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO8097898.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            9427..10548
                     /gene="gmd"
                     /locus_tag="ACHK01_002977"
     CDS             9427..10548
                     /gene="gmd"
                     /locus_tag="ACHK01_002977"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO8097899.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10551..11516
                     /gene="fcl"
                     /locus_tag="ACHK01_002978"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10551..11516
                     /gene="fcl"
                     /locus_tag="ACHK01_002978"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO8097900.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11519..11998
                     /gene="gmm"
                     /locus_tag="ACHK01_002979"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11519..11998
                     /gene="gmm"
                     /locus_tag="ACHK01_002979"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO8097901.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11995..13218
                     /gene="wcaI"
                     /locus_tag="ACHK01_002980"
     CDS             11995..13218
                     /gene="wcaI"
                     /locus_tag="ACHK01_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO8097902.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13221..14657
                     /gene="cpsB"
                     /locus_tag="ACHK01_002981"
     CDS             13221..14657
                     /gene="cpsB"
                     /locus_tag="ACHK01_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO8097903.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14850..16220
                     /gene="cpsG"
                     /locus_tag="ACHK01_002982"
                     /gene_synonym="manB"
     CDS             14850..16220
                     /gene="cpsG"
                     /locus_tag="ACHK01_002982"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO8097904.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16275..17669
                     /gene="wcaJ"
                     /locus_tag="ACHK01_002983"
     CDS             16275..17669
                     /gene="wcaJ"
                     /locus_tag="ACHK01_002983"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO8097905.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSMIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17671..19149
                     /gene="wzxC"
                     /locus_tag="ACHK01_002984"
     CDS             17671..19149
                     /gene="wzxC"
                     /locus_tag="ACHK01_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO8097906.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19522..20802
                     /gene="wcaK"
                     /locus_tag="ACHK01_002985"
     CDS             19522..20802
                     /gene="wcaK"
                     /locus_tag="ACHK01_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO8097907.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCNALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARHNEAVSRERHTGMQMVQSVLERIGEVK"
     gene            20799..22019
                     /gene="wcaL"
                     /locus_tag="ACHK01_002986"
     CDS             20799..22019
                     /gene="wcaL"
                     /locus_tag="ACHK01_002986"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO8097908.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGLVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22030..23424
                     /gene="wcaM"
                     /locus_tag="ACHK01_002987"
     CDS             22030..23424
                     /gene="wcaM"
                     /locus_tag="ACHK01_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO8097909.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINIGIFIPAGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMTGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23599..24492
                     /gene="galF"
                     /locus_tag="ACHK01_002988"
     CDS             23599..24492
                     /gene="galF"
                     /locus_tag="ACHK01_002988"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO8097910.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24864..25940
                     /gene="rfbB"
                     /locus_tag="ACHK01_002989"
     CDS             24864..25940
                     /gene="rfbB"
                     /locus_tag="ACHK01_002989"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8097911.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            25937..26809
                     /gene="rfbA"
                     /locus_tag="ACHK01_002990"
     CDS             25937..26809
                     /gene="rfbA"
                     /locus_tag="ACHK01_002990"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8097912.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFMRLLGDGSQFGINLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLMLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKCIKPSKRGELEITAVNEVYLRNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNHDELQAKGEFLKKN
                     HYGQYLLRLIKSHE"
     gene            26802..27209
                     /locus_tag="ACHK01_002991"
     CDS             26802..27209
                     /locus_tag="ACHK01_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFO8097913.1"
                     /translation="MNNNVKLIHFDKKGDNRGFLVVVEQNKDIPIEIKRVYYMFDTGS
                     DVRRGYHAHKKLTQIAIPVKGSCKFLLDDGKSVEHVTLDSPSVGLIIEPMIWHEMYDY
                     SSDCVLMVLADDFYDESDYIRNYADFIREITNA"
BASE COUNT         6837 a         6628 c         7297 g         6532 t
ORIGIN      
        1 aattcttaaa aatagccaat tgccgaattg ttaccttgcc tgctattccg ttagctgtaa
       61 cacttcctcc tgcattattg gagagccaat attcaattac cataatgtca ccttatattc
      121 ggaagttaat ttccgcaata accctttctc atcaacgatt gtatgcagag agggtttgaa
      181 aaattaagct aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat
      241 gcctgaaaga ccagtctgaa ttattctgtc aatagtctgc ggatgaaatc aattttttta
      301 ggactgatgc cagttaaatt tttattcaat taactgcaag gtaaatgtgc atggcgacat
      361 tgctattaat agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa
      421 tccaaaatga aattgatgcc attattggtg tcagtaacct tgataagcgg ttgcacagta
      481 cttccgggca gcaatatgtc gacgatgggc aaagacgtca tcaaacagca ggacgctgat
      541 ttcgatctcg acaaaatggt gaatgtttat ccgctgaccc cgcgcctgat tgaccaatta
      601 cgcccacgcc cgaatgtagc gcgccccaat atgacgctgg aaagtgagat cgcgaattac
      661 cagtatcgcg tcgggccggg agacgttctt aatgtcaccg tctgggatca cccggaactc
      721 accacgccag ctggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac
      781 ggcactatgt tctacccgta tatcggcaag attcacgtcg tggggaaaac gctcgctgaa
      841 atccgcagtg atattaccgg gcgcttagcg acgtacatcg ctgacccgca ggtggacgtt
      901 aatatcgccg ccttccgctc acaaaaggcc tatatctccg gtcaggtgaa taaatccggt
      961 caacaggcga tcaccaacgt accactgacc attctcgatg ccatcaacgc cgcaggtggc
     1021 ctgaccgaca ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg tgaagaacgc
     1081 atttctttgc aggcgctgat gcaaaacggc gacctcaatc agaaccgact gctttacccc
     1141 ggcgatattc tctacgtgcc gcgtaatgat gatctgaaag tatttgtgat gggtgaagtg
     1201 aagaaacaga gcaccctgaa aatggacttt agcggcatga ccctgacgga agccttgggt
     1261 aatgctgaag gcatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg
     1321 ctgaaaggcg agggcgggcg taacggcaag attgccaata tctaccagct ggatatgtcc
     1381 gatgcgacgt cgctggtgat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat
     1441 gtcaccaccg ccccggtttc ccgctggaac cgtctgatca atcagttgct gccaactatt
     1501 agcggtgtcc gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt
     1561 ttaacaacat tttagttgtc tgtgtcggca atatttgccg ttccccgacg gcggaacgct
     1621 tactgcaacg ttatcacccg gaactgaaag tggagtccgc tggactcggc gcgctggtcg
     1681 gtaagggcgc tgaccctact gctatcagcg tcgccgcaga acatcaactg tctctggaag
     1741 gtcactgtgc ccgtcaaatc agccgccgcc tgtgtcgcaa ctacgacctg attttgacca
     1801 tggaaaagcg ccatatcgaa cgcttatgcg agatggcacc cgagatgcgc ggcaaagtga
     1861 tgctgtttgg tcactgggat aacgaatgtg aaatccccga tccgtatcgc aaaagccggg
     1921 aaacgtttgc agcggtgtac acattacttg aacggtctgc ccgccagtgg gcgcaggcat
     1981 tgaacgcaga gcaggtataa gaatgacaga aaaagtaaaa caacatgccg ctccggtaac
     2041 gggcagtgat gaaatcgata ttggtcgcct ggtcggcacc gtcattgaag cgcgctggtg
     2101 ggtgattggc atcaccgccg tatttgccct ctgtgccgtg gtttacactt tcttcgccac
     2161 gccgatttat agtgccgacg cactggtaca aatcgagcaa agcagcggca attcgttagt
     2221 gcaggacatt ggttcggcat tagccaacaa accgcctgca tcggacgccg agatccagtt
     2281 gattcgttcg cgtctggtgc ttggtaaaac ggtggacgat ctcgacctcg atattgcagt
     2341 gagcaaaaac acgttcccga ttttcggggc gggctgggat cgcctgatgg gacgccagaa
     2401 cgagacggtg aaagtgacta cctttaaccg cccgaaagag atggcggatc aggtgtttac
     2461 gcttaatgtg ctggatgaca aaaactacac cctgagcagt gatggcggct ttagcgcccg
     2521 tggacaagcg ggccagatgc tgaaaaaaga aggcgtcacg ctgatggttg aagccattca
     2581 cgcccgcccg ggcagtgagt ttaccgtcac caaatactcc acgctgggga tgatcaatca
     2641 actgcaaaac agcctgacgg taacggagaa cggcaaagac gcaggcgtac tgagcctgac
     2701 ttataccggt gaagatcgcg aacagatccg cgacattctt aatagcatcg cccgtaacta
     2761 tcaggaacaa aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg ctttcctcgc
     2821 gcaacagtta ccggaagtac gtagccgcct tgatgttgcc gaaaacaaac tgaatgcctt
     2881 ccgtcaggat aaagattctg ttgatctgcc gctggaagcg aaagcggtgc tcgattcgat
     2941 ggtgaacatc gacgcccagt tgaacgaact gacctttaaa gaggcggaaa tctccaagct
     3001 gtacaccaaa gttcacccgg cgtatcgcac gctgctggag aaacggcagg cgctggaaga
     3061 cgaaaaagcc aaacttaacg gtcgcgtaac ggcgatgccg aaaacccagc aggaaattgt
     3121 ccgtctgacc cgcgatgtcg agtctgggca gcaggtctat atgcaactgc tgaataaaga
     3181 gcaggagctg aaaatcaccg aagccagcac cgtcggcgat gtgcgcattg ttgacccggc
     3241 aatcacccag cctggcgtgc tgaaaccgaa gaaaggactg attatcctcg gcgcaattat
     3301 cctcggcctg atgctctcta tcgtgggtgt gctgctgcgc tcgttgttta atcgcggcat
     3361 cgaaagcccg caggtgctgg aagaacatgg tatcagcgtc tatgccagca tcccgttgtc
     3421 ggaatggcag aaagcgcgcg atagcgtcaa aaccatcaaa gggattaaac gctataaaca
     3481 gagccagcta ctggcggtgg ggaatccaac cgatctggcg attgaagcca tccgcagtct
     3541 gcgtaccagt ctgcacttcg cgatgatgca ggcgcagaac aatgtgttga tgatgacagg
     3601 ggtgagcccg tcaatcggta aaacctttgt ctgcgccaac ctggcggcgg tgatcagtca
     3661 gaccaataaa cgggtgttgt tgatcgactg cgatatgcgc aaaggctaca cccacgagct
     3721 gttgggtacc aataacgtca atggcctgtc ggaaattctg attggccagg gcgatattac
     3781 taccgcagct aaaccgacct ctattgccaa atttgacctg atcccgcgcg gtcaggtacc
     3841 gccaaatcct tctgaactgt tgatgagcga acgctttgcc gaactggtga actgggcgag
     3901 taaaaactat gacctggtgt tgattgatac gccgccgatt ctggcagtga ccgatgcggc
     3961 aattgttggt cgtcatgttg gaaccacgtt aatggtggcg cgttatgcgg tcaacacatt
     4021 gaaagaagtg gaaaccagtc tgagccgctt tgagcaaaat ggtattccgg tgaaaggggt
     4081 gattctgaac tccatcttcc gccgcgccag cgcgtatcag gattatggct attacgaata
     4141 cgaatataag tcggatgcga aataacgaca tccgctgtcg gatgcgatgc tgacgcatct
     4201 tatccgacct accttcacgc cgcatccggc aagcaaacca gctccgtagg ccggataagg
     4261 cgctcacgcc gcatccggca acagaacccg taaacaagct tataaaccgg cagaacatct
     4321 cgacactccg ggagaacaac ctatgaaaga caatccgctg atttcaatct atatgccgac
     4381 ctggaaccgc caacaactgg cgattcgggc gataaaatcg gtcctgcgcc aggactacag
     4441 caactgggag atgatcatcg tggatgattg ctccacctcc tgggaacaac tgcaacagta
     4501 cgtcactgcc ctcaacgatc cgcgtattac ctacattcac aacgacatta acagcggggc
     4561 gtgcgcggta cgtaaccagg cgattatgct ggcgcaaggg gaatacatta ccgggatcga
     4621 tgacgatgat gaatggacgc ccaaccgcct gagcgtcttc ctcgcccata aacagcaact
     4681 ggtcactcac gcctttttgt acgctaacga ctacgtttgc cagggcgaag tctattccca
     4741 accagcaagc ttaccactgt atcccaaatc gccgtactca cgccgcctgt tctataaacg
     4801 caatatcatt ggcaatcagg tttttacctg ggcgtggcgt ttcaaagagt gcttgttcga
     4861 taccgaactg aaagcagcac aggattacga catcttcctg cggatggtgg tggagtatgg
     4921 cgaaccgtgg aaagtggaag aggcgacgca gatcctgcat atcaatcacg gtgaaatgca
     4981 gatcacctcg tcaccgaaaa aattctccgg gtacttccac ttttaccgca agcacaaaga
     5041 caaattcgac cgtgccagta aaaaatatca gctgtttacc ctctaccaga tccgcaataa
     5101 acgcatgacc tggcgcactt tgctgacgct gctgtcggtg cgtaacggca aacgtctggc
     5161 tgatggtatt cgggggcgct aaagatgctg gaagatctgc gcgccaacag ctggagttta
     5221 cgcccgtgct gcatggttct tgcctatcgc gttgctcatt tttgttcggt gtggcgcaaa
     5281 aagaacgtcc tcaacaatct gtgggcggcc ccgctgctgg tgctgtatcg cattatcacc
     5341 gaatgctttt tcggttatga aatccaggca gccgcaacca ttggccgccg ctttactatc
     5401 catcacggtt acgccgtagt gatcaataaa aacgtggttg cgggggatga tttcaccatt
     5461 cgccacggcg tcactatcgg caatcggggt gccgataaca tggcatgtcc acacattggc
     5521 aacggcgtcg aactcggtgc caacgtcatt attcttggtg atatcacgct tggtgacaac
     5581 gtcaccgtgg gcgcgggcag cgtggtgctc gactctgtcc cggacaacgc gctggtggtg
     5641 ggagaaaaag cgcgagtgaa ggtaattaaa tgaatatttt gcaatttaat gtgcgactgg
     5701 cggaaggcgg ggcagcaggt gtggcgttag atctccacca gcgtgcgctg caacaggggc
     5761 tggcgtcaca ttttgtctac ggttacggca aaggcggcaa agagagcgtc agccatcaga
     5821 actatccgca ggtcatcaaa catacgccgc ggatgaccgc gatggcgaat attgccctgt
     5881 ttcgtctgtt taatcgcgat ctgtttggca atttcaatga gttatatcgc accattactc
     5941 gcacaccggg tccggtggtc ctgcattttc atgtgctgca cagctactgg ctaaatctta
     6001 agagcgtggt gcgcttttgc gaaaaagtga aaaaccacaa accggatatc actctggtct
     6061 ggacgctgca cgatcactgg agcgttaccg gacgctgcgc ctttaccgac ggttgcgaag
     6121 gctggaaaac gggctgccag aaatgcccga ccttaaataa ctatccgccg gtgaagattg
     6181 atcgcgcgca tcagctggtg gcgggcaaac gccagttatt ccgcgagatg ctggcgctgg
     6241 gctgtcagtt tatttccccc agccagcatg tggctgacgc tttcaatagt ctgtacgggc
     6301 cagggcgttg ccggattatc aataatggca ttgatatggc aaccgaagcg attctggcag
     6361 atctgcctcc ggtgcgtgaa acccagggca agccgaaaat cgcggtggtg gcgcatgact
     6421 tgcgttacga cggcaagact aaccagcaac tggtgcgtga gatgatggcg ctgggcgaca
     6481 aaatcgaact gcataccttt ggtaagttct cgccgttcac cgctggcaac gtggttaatc
     6541 acggctttga aactgacaag cgcaagttga tgagcgcgct caatcagatg gatgcgctgg
     6601 tattcagttc tcgcgtcgat aactacccgc tgattttgtg tgaggcgtta tcaattggcg
     6661 tgccggtgat tgccacccat agcgacgcgg cacgggaagt gttgcaaaaa tccggcggta
     6721 aaaccgtcga cgaagaagag gtgctgcaac tggtccagtt aagcaaaccg gaaatcgcgc
     6781 aggcgatatt tggtaccacg ctggctgagt tcagccaacg cagccgcgcc acctacagtg
     6841 gacaacagat gctggaggag tatgtcaact tctatcagaa tctgtagcta cctgctgctg
     6901 ccgctgatct acctgctggt taacgtcaaa atcgcccagc ttggcgaaag tttccccatc
     6961 actatcgtca cttttttacc tgtcttgtta ctgctgtttt tagaacgcat cagcgttaaa
     7021 aaattgatga ttgccttagg gattggtgcg ggactcacgg cgttcaacta tctgtttggt
     7081 cagtcgttgg atgccagtaa atacgtcaca tcaaccatgc tgtttgtcta tattgtgatc
     7141 attattggca tggtgtggag tattcgcttt aaaacgattt cgccacacaa ccatcgcaag
     7201 atattacgtt tcttttatct ggtggtcggg ctggtggtgg cgctggcggc ggtggagatg
     7261 gcgcaaatta tcctcaccgg tggcagcagt attatggagt cgatttcgaa atatctgatt
     7321 tacagcaaca gctatgtact gaatttcatt aaattcggcg gcaagcgcac gacagcactt
     7381 tatttcgaac cggcattttt cgctctggca ttaatctcaa tttggctcag catcaaacag
     7441 tttggtatca aaacgcctaa aaccgatgct atgattctcg cagggataat attatccgga
     7501 tcgttttcag gggttatgac ctttatcctg ttttatttgc tggagtgggc atttcaatat
     7561 ctgaataaag aggcgattaa gaaaaagtta ccgttggcat tgatttctct ggctgtattc
     7621 ctggttggtg tggtaatcgc gtttccttat atttccaccc gtctgggcga tttaggtacg
     7681 gaaggatcgt catcgtatta tcgtattgtc ggtccgctgg tgatggtcgg ttattctttg
     7741 acccatattg acggtgtagt cagatttggc tcactttatg aatacgtcgc atcattcgga
     7801 atatttaacg gtgcggatgt cggaaaaacc atagacaatg gtttgtatct gctgattatt
     7861 tatttttcct ggtccgcagt gtttttatcg ctgtggtaca tggggaaagt gataaaaatg
     7921 atgatcaacg cttttggtga taaccgcaat tttcgcgtgc aattatatct ttttactccg
     7981 gtatcgctgt tttttaccgg ttcgatattt agcccggaat atgcattttt aatcgtctgt
     8041 ccgtttattt tgcgaaaagc gttaaatatt acgaggtaag aataagaaca tgttgcttag
     8101 cataatcact gtcgcgtttc gtaacctcga agggatagtc aaaacacatg cctcgctggc
     8161 gcatctggcg caggcggaag atatcagctt cgaatggatt gttgtcgatg gcggttccaa
     8221 tgacggcacc cgtgagtatc tggaaaatct caatggtatc tataacctac gctttgtcag
     8281 cgagccagat aacggtatct acgacgccat gaacaaaggc attacgatgg cacaaggcaa
     8341 gttcgcgttg tttctcaatt cgggcgatat ttttcatcag gatgccgcaa attttgtccg
     8401 taagttaaaa atgcaaaaag ataacatgat gatcaccggc gatgcgctgc tggattttgg
     8461 cgacgggcat aaaattaaac gtagcgccaa accgggctgg tatatttatc acagcctgcc
     8521 cgccagtcat caggcgatat ttttccctgt atccggcttg aaaaaatggc gttatgacct
     8581 ggaatataaa gtttcttccg actacgcgct ggcagccaaa atgtataaag ccggttatgc
     8641 atttaaaaaa ctcaatggcc tggtgtctga attttccatg ggtggggtat ctaccaccaa
     8701 taatatggaa ttgtgtgctg acgcgaaaaa agtccaacga caaatattac atgtacctgg
     8761 cttttgggct gaattatcct ggcatttacg ccaacgtact acctcaaaga cgaaagcctt
     8821 atataacaaa gtctgaatat aaggagaacc aatgcaagat ttaagcggtt tctcggtgcc
     8881 gaaagggttc cggggcggca acgctattaa agtgcaatta tggtgggcag tacaggcaac
     8941 aatatttgcc tggtcgccac aagtattgta tcgctggcgg gcatttttat tacgtttatt
     9001 cggcgcaaaa ataggaaaaa acgtagttat tcgtccgtca gtaaaaatta cctatccgtg
     9061 gaaattaacc ttaggtgatt acgcgtgggt cggcgatgac gtcaatttat ataccctcgg
     9121 tgaaataacc attggcgcac attcggtgat atcgcaaaaa agttatttat gcaccggtag
     9181 ccacgaccat gcaagtcaac atttcaccat taatgctacg cctattgtga ttggcgagaa
     9241 atgctggctg gcaaccgatg tttttgttgc ccctggcgtc acgattggcg acggcaccgt
     9301 cgtgggtgca cgaagcagtg tttttaaaac gcttccggca aatgtggttt gccgggggaa
     9361 tcccgcagtg gtgatacgcg aacgcgttga aactgaataa attcaaaaaa tacagaggaa
     9421 taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg ttcttacctg
     9481 gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg tgcatcgtca
     9541 ttcaacaccg agcgcgtgga tcacatttat caggatccgc acacctgcaa cccgaaattc
     9601 catctgcatt atggcgacct gagtgatacc tccaacctga cacgcatttt gcgtgaagtg
     9661 cagccggatg aagtttacaa cctgggcgca atgagccacg ttgcggtttc ttttgagtca
     9721 ccggaatata ccgcagacgt cgatgcgatg ggtacgctgc gcctgctcga ggcgatccgc
     9781 ttcctcggtc tggaaaagaa aacccgtttc tatcaggctt ccacctctga actgtatggt
     9841 ctggtgcagg aaattccgca gaaagaaacc acgccgttct acccacgctc tccgtatgct
     9901 gtcgccaaac tgtacgccta ctggatcacc gttaactacc gcgaatccta cggcatgtac
     9961 gcctgtaacg gtattctctt caaccatgaa tccccgcgcc gcggcgaaac cttcgttacc
    10021 cgcaaaatca cccgcgcaat cgccaatatc gcccaggggc tggagtcgtg cctgtacctc
    10081 ggcaatatgg attccctgcg tgactggggc catgccaaag actacgtaaa aatgcagtgg
    10141 atgatgctgc aacaggaaca gccggaagat ttcgttatcg cgaccggcgt tcagtactcc
    10201 gtgcgtcagt tcgtggaaat ggcggcagca cagctgggca tcaaactgcg ctttgaaggc
    10261 acgggcgttg aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc gccgggcgtt
    10321 aaaccgggcg atgtgattat cgccgttgac ccgcgttact tccgtccggc agaagttgaa
    10381 acgctgctcg gcgacccgac caaagcgcac gaaaaactgg gctggaaacc ggaaatcacc
    10441 ctcagagaga tggtgtctga aatggtggct aatgacctcg aagcggcgaa aaaacactct
    10501 ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc atgagtaaac
    10561 aacgcatttt tatcgctggc catcgtggga tggtcggttc tgccatcagg cggcagctcg
    10621 aacagcgcgg tgatgtggaa ctggtattac gcacccgcga cgagctgaac ctgctggaca
    10681 gccgcgcggt gcatgatttc tttgccagcg aacgcattga ccaggtctat ctggcggcgg
    10741 cgaaagtggg cggcattgtt gccaacaaca cctatccggc ggatttcatc taccagaaca
    10801 tgatgattga gagcaacatc attcacgccg cgcatcagaa cgacgtgaac aaactgctgt
    10861 ttcttggatc gtcctgtatc tacccgaaac tggcaaaaca gccgatggca gaaagcgagt
    10921 tattgcaggg cacgctggag ccgaccaacg agccttacgc cattgccaaa atcgccggga
    10981 tcaaactgtg cgaatcatac aaccgccagt acggacgcga ttaccgctca gtcatgccga
    11041 ccaacctgta tggcccgcat gacaacttcc acccgagtaa ttcgcatgtg atccctgcat
    11101 tgctgcgtcg cttccacgag gcgacggcac agaatgcacc ggacgtggtg gtatggggca
    11161 gcggtacacc gatgcgtgaa ttcctgcacg tcgatgatat ggcggcggcg agcattcatg
    11221 tcatggagct ggcgcacgaa gtctggctgg agaacaccca gccgatgctg tcgcacatta
    11281 acgtcggcac gggcgttgac tgcactatcc gcgagctggc gcaaaccatc gccaaagtgg
    11341 tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg ccgcgcaaac
    11401 tgctggatgt gacgcgcctg catcagcttg gctggtatca cgaaatctca ctggaagcgg
    11461 ggcttgccag cacttaccag tggttccttg agaatcaaga ccgctttcgg gggtaatgat
    11521 gtttttacgt caggaagact ttgccacggt agtgcgctcc accccgcttg tctctctcga
    11581 ctttattgtc gagaacagtc gcggcgagtt tctgcttggc aaaagaacca accgcccggc
    11641 gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc tggaagccgc
    11701 attcgagcgg ctgacgatgg cggaactggg gctgcgtctg ccgataacag caggccagtt
    11761 ttacggtgtc tggcagcact tttatgacga taacttctcc ggcacggatt tcaccactca
    11821 ctatgtggtg ctcggttttc gcttcagagt cgctgaagaa gatctgatac tgccggatga
    11881 gcagcatgac gattaccgct ggctgacgcc ggacgcgctg ctcgccagta acgatgttca
    11941 cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg gattatgaaa
    12001 atactggtct acggcattaa ctactcgccg gagttaaccg gtatcggcaa atacaccggc
    12061 gagatggtgg aatggctggc ggcacaaggt catgaggtgc gggttattac cgcaccgcct
    12121 tactacccgc agtggcaggt gggcgagaac tattccgcct ggcggtacaa acgagaagag
    12181 ggggccgcca cggtgtggcg ctgcccgctg tacgtgccaa aacagccgag caccctgaaa
    12241 cgcctgttac atctcggcag ttttgccgtc agcagtttct ttcctctgat ggcgcaacgt
    12301 cgctggaagc cggatcgcat tatcggcgtg gtgccaacgc tgttttgcac gccgggaatg
    12361 cgcctgctgg cgaaactttc tggtgcgcgt accgtgctgc atattcagga ttacgaagtg
    12421 gacgccatgc tggggctggg ccttgccgga aaaggcaaag gcagcaaagt ggcacagctg
    12481 gcaacggcgt tcgaacgtag cggactgcat aacgtcgata acgtctccac gatttcgcgt
    12541 tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg aaaacgtcat cttcttcccc
    12601 aactggtcgg aaattgcccg ttttcagcat gttgcagatg ccgatgttga tgcccttcgt
    12661 aaccagcttg acctgccgga taacaaaaaa atcattcttt actccggcaa tattggtgaa
    12721 aagcaggggc tggaaaacgt tattgaagct gccgatcgtc tgcgcgatga accgctgatt
    12781 tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc gcagcagcgt
    12841 ggactgcgca acatgcaatt tttcccgctg caatcgtatg acgctttacc cgcactgctg
    12901 aagatgggcg attgccatct ggtggtgcaa aaacgcggcg cggcagatgc cgtattgccg
    12961 tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg tgattaccgc ggaggcccac
    13021 acagaactgg ggcagctttg cgaaaccttt ccgggcattg cggtttgcgt agaaccggaa
    13081 tcggtcgagg cgctggtggc ggggatccgt caggcgctcc tgctgcccaa acacaacacg
    13141 gtggcacgtg aatatgccga acgcacactc gataaagaga acgtgttacg tcaatttata
    13201 aatgatattc ggggataatt atggcgcagt cgaaactcta tccagttgtg atggcaggtg
    13261 gctctggtag ccgcttatgg ccgctttccc gcgtacttta ccccaagcag tttttatgcc
    13321 tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg cctgaatggc gtggagtgcg
    13381 aaagcccggt ggtgatttgc aatgagcagc accgctttat tgtcgcggaa cagctgcgtc
    13441 aactgaacaa acttaccgag aacattattc tcgaaccggc agggcgaaac acggcacctg
    13501 ccattgcgct ggcggcgctg gcggcaacac gtcatagccc ggagagcgac ccgttaatgc
    13561 tggtattggc ggcggatcat gtgattgccg atgaagacgc gttccgtgcc gccgtacgta
    13621 atgccatgcc gtatgccgaa gcgggcaagc tggtgacctt cggcattgtg ccggatctac
    13681 cagaaaccgg ttatggctat attcgtcgcg gtgaagtgtc tgcgggtgag caggatacgg
    13741 tggcctttga agtggcgcag tttgtcgaaa aaccgaatct ggaaaccgcc caggcctatg
    13801 tggcaagcgg cgaatattac tggaacagcg gcatgttcct gttccgcgcc ggacgctatc
    13861 tcgaagaact gaaaaaatat cgtccggata ttctcgatgc ctgtgaaaaa gcgatgagcg
    13921 ccgtcgatcc ggatctcgat tttattcgtg tggatgaaga agcgtttctc gcctgcccgg
    13981 aagagtcggt ggattacgcg gtcatggaac gtacggcaga tgccgttgtg gtgccgatgg
    14041 atgcgggctg gagcgatgtc ggctcctggt cctcattatg ggaaattagc gcccacaccg
    14101 ccgagggcaa cgtttgccac ggcgatgtga ttaatcacaa aactgaaaac agctatgtgt
    14161 acgccgaatc tggcctcgta accaccgtcg gggtgaaaga tctggtagtg gtgcagacca
    14221 aagatgcggt gctgattgcc gaccgtaacg cggtgcagga tgtgaaaaaa gtggtcgagc
    14281 agatcaaagc cgatggtcgc catgagcatc gggtgcatcg cgaggtctat cgcccgtggg
    14341 gcaaatatga ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc accgtgaaac
    14401 cgggcgaggg cttgtcggta cagatgcacc atcaccgcgc ggaacactgg gtagtggtcg
    14461 cgggaacggc aaaagtcact attgacggtg atatcaaact gcttggtgaa aacgagtcca
    14521 tttatattcc gctgggggcg acgcactgcc tggaaaaccc ggggaaaatt ccgctcgatt
    14581 taattgaagt gcgctccggc tcttatctcg aagaggatga tgtggtgcgc ttcgcggatc
    14641 gctacggacg ggtgtaaacg tcgcatcagg cgctgtttgt cggatgcggc gtgaacgcct
    14701 tatccgacct acggttcgga tttgtaggcc tgataagacg cggcagcgtc gcatcaggca
    14761 atgaatgcga aaccgagatg taaataacga caaaaacaaa attggccgtt tcggtcaggg
    14821 ccaactattg cctgaaaaag ggtaacgaca tgaaaaaatt aacctgcttt aaagcctatg
    14881 atattcgcgg gaaattaggc gaagaactga atgaagatat tgcctggcgc attgggcgtg
    14941 cctatggcga atttctcaaa ccgaaaacca ttgtgttagg cggtgatgtc cgtctcacca
    15001 gcgaaacctt aaaactggcg ctggcaaaag gtttacagga tgcgggcgtt gacgtgctgg
    15061 atatcggtat gtccggcacc gaagagatct atttcgccac gttccatctc ggcgtggatg
    15121 gcggcattga agttaccgcc agccataatc cgatggatta taacggcatg aagctggttc
    15181 gcgagggggc tcgcccgatc agcggagata ccggactgcg cgacgtccag cgtctggctg
    15241 aagccaacga ctttcctccc gtcgatgaaa ccaaacgcgg tcgctatcag caaatcaacc
    15301 tgcgtgacgc ttacgttgat cacctgttcg gttatatcaa tgtcaaaaac ctcacgccgc
    15361 tcaagctggt gatcaactcc gggaacggcg cagcgggtcc ggtggtggac gccattgaag
    15421 cccgctttaa agcccttggc gcacccgtgg aattaatcaa agtgcacaac acgccggacg
    15481 gcaatttccc caacggtatt cctaacccgc tactgccgga atgccgcgac gacacccgca
    15541 atgcggtcat caaacacggc gcggatatgg gcattgcctt tgatggcgat tttgaccgct
    15601 gcttcctgtt tgacgaaaaa gggcagttta tcgaaggcta ctacattgtc ggcctgctgg
    15661 cagaagcgtt cctcgaaaaa aatcccggcg cgaagatcat ccacgatcca cgtctctcct
    15721 ggaataccgt tgatgtggtg actgccgctg gcggcacgcc ggtgatgtcg aaaaccggac
    15781 acgcctttat taaagaacgt atgcgcaagg aagacgccat ctacggtggc gaaatgagcg
    15841 ctcaccatta cttccgtgat ttcgcttact gcgacagcgg catgatcccg tggctgctgg
    15901 tcgccgaact ggtgtgcctg aaaggaaaaa cgctgggcga actggtgcgc gaccggatgg
    15961 ctgcgtttcc ggcaagcggt gagatcaaca gcaaactggc gcaacccgtt gaggcgatta
    16021 atcgcgtcga acagcatttt agccgtgagg cgctggcggt ggatcgtacc gatggcatca
    16081 gcatgacctt tgccgactgg cgctttaacc tgcgcacctc caacaccgaa ccggtggtgc
    16141 ggttgaatgt ggaatcgcgc ggtgatgtgc cgctgatgga agcgcgaacg cgaactctgc
    16201 tgacgttgct gaatgagtaa tgtcggatct tcccttaccc cactgcgggt aaggggctaa
    16261 taacaggaac aacgatgaca aatctaaaaa agcgcgagcg agcgaaaacc aatgcatcgt
    16321 taatctctat ggtgcaacgc ttttcagata tcaccatcat gtttgccgga ctatggctgg
    16381 tttgcgaagt cagcggactg tcattcctct acatgcacct gttggtggcg ctgattacgc
    16441 tggtggtgtt ccagatgctg ggcggcatca ccgattttta tcgctcatgg cgcggtgttc
    16501 gggcagcgac agaatttgcc ctgttgctac aaaactggac cttaagcatg attttcagcg
    16561 ccggactggt ggcgttcaac aatgatttcg acacgcaact gaaaatctgg ctggcgtggt
    16621 atgggctgac cagtatcgga ctggtggttt gccgttcatg tattcgtatt ggggcgggct
    16681 ggctgcgtaa tcatggctat aacaagcgca tggtcgccgt agcgggggat ttagccgccg
    16741 gacaaatgct gatggagagc ttccgtaatc agccgtggtt agggtttgaa gtggtgggcg
    16801 tttaccacga cccgaaaccg ggcggcgttt ctaacgactg ggcgggcaat ctgcaacagc
    16861 tggtcgagga tgctaaagcg ggcaagattc ataacgtcta tatcgcgatg caaatgtgcg
    16921 acggcgcgcg agtgaaaaaa ctggtccatc aactggcgga caccacctgt tcggtgctgc
    16981 tgatccccga tgtctttacc ttcaatattc tccattcacg tctcgaagag atgaacggcg
    17041 ttccggtggt gccgctgtat gacacgccgc tttccggggt taaccgcctg ctaaaacgtg
    17101 cggaagacat tgtgctggcg acgcttatcc tgctgctgat ctccccggtg ctgtgctgca
    17161 ttgcgctggc ggtgaaactc agttcacctg ggccggttat tttccgccag actcgctacg
    17221 gcatggatgg caagccgatc aaagtgtgga agttccgttc catgaaagtg atggagaacg
    17281 acaaagtggt gacccaggcg acgcagaacg atccgcgcgt cactaaagtg gggaactttc
    17341 tgcgccgcac ctcgctggac gaattgccgc agtttatcaa tgtgctgacc ggggggatgt
    17401 cgattgtcgg tccacgtcca cacgcggtgg cgcataacga acagtatcgc cagctcattg
    17461 aaggctacat gctgcgtcat aaagtgaaac ccggcattac cggctgggcg cagattaatg
    17521 gctggcgcgg cgaaaccgac acgctggaga aaatggaaaa acgcgtcgag ttcgaccttg
    17581 agtacatccg cgaatggagc gtctggttcg atatcaaaat cgttttcctg acggtattca
    17641 aaggcttcgt taacaaagcg gcatattgat atgagcttac gtgaaaaaac catcagcggc
    17701 gcgaagtggt cggcgatagc cacggtgatc atcatcggcc tcgggctggt gcagatgacc
    17761 gtgctggcgc ggattatcga caaccaccag ttcggtttgc ttaccgtgtc gctggtgatc
    17821 atcgcgctgg cagatacgct ttctgacttc ggtatcgcta actcgattat tcagcgaaaa
    17881 gaaatcagcc accttgaact caccacgctg tactggctga acgtcgggct ggggcttgtg
    17941 gtgtgcgtgg cggtgttttt gttgagtgat gccattggcg acgtgctgaa taacccggac
    18001 ctggcaccgt tgattaaaac attgtcgctg gcgtttgtgg tgatccccca cgggcaacag
    18061 ttccgcgcgt tgatgcagaa ggagctggag ttcaacaaaa tcggcatgat cgaaaccagc
    18121 gcggtactgg cgggcttcac ttttacggtg gttagcgccc atttctggcc gctggcgatg
    18181 accgcgatcc tcggttatct ggtcaatagt gcagtaagaa cgctgctgtt tggttacttt
    18241 ggccgcaaaa tttatcgccc cggtctgcat ttctcgctgg cgtcggtggc accgaactta
    18301 cgttttggtg cctggctgac ggcggacagc atcatcaact atctcaatac caacctttca
    18361 acgctggtgc tggcgcgtat tctcggcgcg ggcgtggcag ggggatacaa cctggcgtac
    18421 aacgtggccg ttgtgccgcc gatgaagctg aacccaatca ttacccgcgt gttgtttccg
    18481 gcatttgcca aaattcagga cgacaccgaa aagctgcgcg ttaacttcta caagctgttg
    18541 tcggtagtgg ggattatcaa ctttccggcg ctgctggggc tgatggtggt gtcgaataac
    18601 tttgtaccgc tggtctttgg tgagaagtgg aacagcatta ttccggtgct gcaattgctg
    18661 tgtgtggtgg gtctgctgcg ctccgtaggt aacccgattg gttcgctgct gatggcgaaa
    18721 gcgcgggtcg atatcagctt taaattcaac gtattcaaaa catttctgtt tattccggcg
    18781 attgttatag gtgggcagat ggcgggcgcg atcggcgtca cgcttggctt cctgctggtg
    18841 caaattatca acaccattct gagttacttc gtgatgatta aaccggttct tggttccagt
    18901 tatcgtcagt acatcctgag tttgtggctg ccgttttacc tctcgctgcc gacgctgggg
    18961 gtcagttatg cgctgggcat tgtgctgaaa gggcaattgg cgctggggat gctgctggcg
    19021 gtgcaaatag ccgcgggcgt gctggcgttt gtggtgatga ttgtgctgtc gcgccatccg
    19081 ctggtggtgg aagtgaagcg tcagttttgt cgcagcgaaa aaatgaaaat gcttttacgg
    19141 gcggggtgag ttgctgtttt cggttccgac aacggttgcc ggatgcggcg taaaacgcct
    19201 tatccgtcct acggttctgt gctcgggacc tgtaggcctg ataagacgcg tcagcgtcgc
    19261 atcaggcact gaatgccgga tgcggcgtga acgccttatc cggcctacgg ttcagggctt
    19321 ggatcttgta ggcccgataa gacgcgccag cgtcgcatca ggcactgaat gccggatgcg
    19381 gcgtaaaacg ccttatccgg cctacggttc tgtgctcggg tcttgtaggc ctgataagac
    19441 gcgccagcgt cgcatcaggc aaaaaacggc aacgaacaca acaaacacag cctcacctgg
    19501 catcaaattt atgaggaaaa aatgaaatta cttattctgg gcaaccacac ttgcggcaat
    19561 cgtggcgaca gcgccatcct gcgcggctta cttgatgcca tcaacatcct caatccacac
    19621 gccgaagtgg atgtgatgag ccgctatccg gtcagttctt cctggctgct caaccgcccg
    19681 gtaatgggcg atccgctgtt cctgcaaatg aaacaacaca acagcgcggc gggcgttgtc
    19741 gggcgcgtta aaaaagtcct ccgtcgccgc tatcagcacc aggtattgct ctcacgcgtc
    19801 accgacactg gcaagctgcg caatatcgcc atcgcccagg gattcaccga cttcgtgcgc
    19861 ctgctctcag gttacgacgc cattattcag gtcggcggat cgttttttgt cgatctctac
    19921 ggcgtgccgc agtttgaaca tgcgctttgc acatttatgg cgaaaaagcc gctgtttatg
    19981 attggtcaca gcgtcggtcc cttccaggat gagcaattta accaactggc gaactacgtt
    20041 tttggtcact gtaacgcgct gatcctgcgc gaatcggtca gtctcgaact gatgaaacgc
    20101 agcaatatca ccactgcaaa agtggaacat ggcgtcgata ccgcgtggct ggtcgatcac
    20161 cacaccgaag atttcaccgc cagctatgcc gtccaacact ggctggacgt tgccgcacaa
    20221 cagaaaacgg tggcaattac cctgcgcgaa ctggcaccgt tcgacaaacg cctcggcacc
    20281 actcagcaag cgtatgaaaa agcctttgcc ggggtggtca atcgcattct cgacgaaggg
    20341 tatcaggtga ttgcgctctc cacctgtacg ggcatcgaca gctataacaa agacgaccgc
    20401 atggtggcgc tcaacctgcg ccagcacatc agcgatcctg cccgttatca cgtagtgatg
    20461 gatgaactca acgatctgga aatgggcaaa attctcggtg cctgtgaact caccgtcggt
    20521 acgcgcctgc actctgccat tatctcgatg aatttctcca ctccggcaat tgccatcaac
    20581 tacgaacata aatccgccgg gattatgcag cagctgggac taccggagat ggcaattgat
    20641 atccgtcatt tattagacgg cagcctgcaa gcgatggttg cggatacctt aggccagctt
    20701 ccggcgctga acgcgcgaca taacgaagct gtcagtcgtg agcgtcatac aggaatgcag
    20761 atggtgcagt ctgtgcttga gcgcatcggg gaggtgaaat gaaggtcggc ttcttcttat
    20821 tgaaatttcc gctgtcgtcg gaaaccttcg tcctcaatca aattaccgcg tttattgata
    20881 tgggctttga ggtggagatt gtcgcgctgc aaaaaggcga cacacaaaac acccacgcgg
    20941 catggacgaa atacaacctt gccgccagaa cccgctggtt acaggacgaa cctacgggca
    21001 aagttgcgaa actgcgccac cgcgccagcc agaccttacg cggcattcat cgtaaaaata
    21061 cctggcaggc gctcaacctc aaacgctatg gtgctgagtc gcgaaacctg attttgtctg
    21121 ccatttgcgg cctggtcgca acaccgtttc atgccgatgt ctttatcgct cattttggcc
    21181 cggcgggggt aaccgcagca aaactccgcg aactgggtgt cattcgcggc aaaattgcca
    21241 ctattttcca cggcattgat atttccagtc gggaagtgct caaccattac actcccgaat
    21301 atcaacaact gtttcgacgt ggcgacctga tgctaccgat aagcgatctg tgggccggaa
    21361 ggctgcaaaa aatgggctgc ccgagggaaa aaatcgccgt atcgcgcatg ggtgtagata
    21421 tgacgcgctt tagcccgcgt cccgtgaaag cgcccgcaac accgctggaa atcatctccg
    21481 tcgcacgctt aaccgaaaaa aaaggcctgc atgtggcgat cgaagcctgc cgtcagttga
    21541 aagagcaggg catggcattt cgctatcgca tcctcggcat tggcccgtgg gaacgacgcc
    21601 tgcgtaccct catcgaacaa tatcaactgg aagatgtggt agagatgccg ggctttaaac
    21661 cgagccacga agtgaaagcg atgctcgacg acgcggatgt cttcctgttg ccatcggtaa
    21721 cgggcgcgga tggcgatatg gaaggcattc cggtagcgct gatggaagcg atggcggtcg
    21781 gcattccggt ggtttctact ctgcatagcg gaataccgga attggtggag gccgataaat
    21841 ccggctggct ggtgcctgag aacgatgctc gcgcactggc gcaacgactg gcggcgttta
    21901 gccaactgga caccgacgaa ctggctccgg tcgtcaaacg tgcgcgcgaa aaagtcgaac
    21961 acgattttaa ccagcaggtg attaaccgag aactcgccag cttgctgcag gctttataga
    22021 ggagaacgta tgccatttaa aaaactctcc cgacgcacct tcctgacggc aagctcggcg
    22081 cttgccttcc tccatacccc tttcgctcgc gcacttcctg ctcgaccaag cgttaatatc
    22141 aacgattaca atccgcacga ctggatcgcc tcatttaaac aagcttttag tgaagggcaa
    22201 acggtcgtcg tgcctgccgg attggtttgt gacaatatca acatcggcat cttcattcct
    22261 gcaggtaaaa cgttacacat ccttggaagc ctacgcggca acggcaaagg gcgatttgtt
    22321 ttacaggacg gcagccaggt gacaggggag gagggcggta gtatgcataa catcaccctg
    22381 gatgtgcgtg gttctgactg caccatcaaa gggctgacga tgaccggctt tggcccggtg
    22441 acgcagatct atatcggcgg caaaaacaaa cgggtcatgc gtaacttgac catcgataac
    22501 ctcactgtca gccacgctaa ttacgccatc ttacgccagg gatttcataa ccagattatc
    22561 ggtgccaaca tcaccaactg taagttcagc gacttacaag gcgacgccat tgaatggaac
    22621 gtggcaatta acgacagtga tattttgatc tccgaccaca tcatcgagcg catcaactgt
    22681 actaacggca aaatcaactg gggcattggc ataggtcttg cgggaagcac ttatgataat
    22741 aattacccgg aagaccaggc agtgaaaaac tttgtcgtgg cgaatatcac gggatcggat
    22801 tgtcggcagt tgatccatgt tgaaaatggt aaacattttg ttatccgtaa tatcaaagcc
    22861 cgcaatatca cgccggattt cagtaagaaa gcaggtattg ataacgcgac agtcgctatt
    22921 tacggttgtg ataatttcgt gattgataat attgaaatga ttaatagtgc cggaatgtta
    22981 atcggctatg gggtaattaa aggcaaatat ctctcgatac cacaaaattt ccgagtgaat
    23041 aatattcaac tggataatac ccaccttgct tacaaattgc gcggtatcca aatctctgcc
    23101 gggaatgccg tctcctttgt ggcactgact aacattgaga tgaagcgtgc gtctctggag
    23161 ttacacaaca aaccgcaaca tctttttatg cgtaatatca aagtgatgca ggaatcctct
    23221 gttggacccg cattgagcat gaactttgac atgcgcaaag acgttcgcgg cgtctttatg
    23281 gcgaaaaaag aaaccctgct atctcttgca aatgttcatg cggtgaatga gaaaggacaa
    23341 agctccgtcg atatcgatag ggttaatcac catattgtta atgtggaaaa gattaacttt
    23401 agattgccgg aacggagaga gtagatttgc gaccattcct ggaaaaatgg agccatactt
    23461 aggaacaatg ctactgcaat ccacaacgaa gcggcgtaac atcacaagta attcagtaat
    23521 caattcaggg taattgatgc tggcgaaaaa aatcgaacaa gctataattc agcaaccatt
    23581 ttacaggtgg atgaaataat gacgaattta aaagcagtta ttcctgtagc gggtcttggg
    23641 atgcatatgt tgcctgccac taaggcgatt cccaaagaga tgctaccgat cgtcgacaag
    23701 ccaatgattc agtacattgt tgacgagatt gtggctgcag ggatcaaaga aatcctcctg
    23761 gtaactcacg cgtccaagaa cgcggtcgaa aaccacttcg acacctctta tgaattagaa
    23821 tctcttcttg aactgcgcgt gaagcgtcag ctgctggcgg aagtgcagtc catctgtccg
    23881 ccgggcgtga ccattatgaa cgtgcgtcag ggcgaacctt taggtttagg tcactccatt
    23941 ttgtgtgcgc ggcctgccat tggtgacaat ccatttgtcg tggtgctgcc agacgttgtg
    24001 atcgacgacg ccagcgccga cccgctgcgc tacaaccttg ctgccatgat tgcacgattc
    24061 aacgaaacgg gtcgtagcca ggtgctggca aaacgtatgc cgggtgacct ctctgaatac
    24121 tcggtcatcc agaccaaaga accactggac cgtgaaggta aagtcagccg cattgttgaa
    24181 tttatcgaaa agccggatca gccgcagacg ttggactcag acatcatggc cgttggtcgc
    24241 tatgtgcttt ctgccgatat ttggccggaa cttgaacgca ctcagcctgg tgcatggggg
    24301 cgtattcagc tgactgatgc catcgctgaa ctggcgaaaa aacagtccgt tgatgccatg
    24361 ctgatgacag gtgacagcta cgactgcggt aaaaaaatgg gctatatgca ggcgttcgtg
    24421 aagtatgggc tgcgcaacct gaaagaaggg gcgaagttcc gtaaagggat tgagaagctg
    24481 ttaagcgaat aatgaaaatc tgaccgaatg taacggttga taagaaaatt attacggcag
    24541 tgaagattcg tggcgaaagt aatttgttgc gaattttcct gccgttgttt tatataaaca
    24601 atcagaataa caacgagtta gcaataggat tttagtcaaa gttttccagg attttccttg
    24661 tttccagagc ggattggtaa gacaattagc gtttgaattt ttcgggttta gcgcgagtgg
    24721 gtaacgctcg tcacatcgtg gacatgtatg cagtgctctg gtagctgtaa agccaggggc
    24781 ggtagcgtgc attaatacct ctattaatca aactgagagc cgcttatttc acagcatgct
    24841 ctgaagtaat atggaataat aaagtgaaga tacttgttac tggtggcgca ggatttattg
    24901 gttctgctgt agttcgtcac attataaata atacgcagga tagtgttgtt aatgtcgata
    24961 aattaacgta cgccggaaac ctggaatcac ttgcagatgt ttctgactct aaacgctatg
    25021 tttttgaaca tgcggatatt tgcgatgctg ctgcaatggc gcggattttt gctcagcatc
    25081 agccggatgc agtgatgcac ctggctgctg aaagccatgt ggatcgttca attacaggcc
    25141 ctgcggcatt tattgaaacc aatattgttg gtacttatgt ccttttggaa gccgctcgca
    25201 attactggtc tgctcttgat agcgacaaga aaaatagctt ccgttttcat catatttcta
    25261 ctgacgaagt atatggtgat ttgcctcatc ctgacgaggt aaataataca gaagaattac
    25321 ccttatttac tgagacaaca gcttacgcgc caagcagccc ttattccgca tcaaaagcat
    25381 ctagcgatca tttagtccgt gcgtggaaac gtacctatgg tttaccgacc attgtgacta
    25441 actgttcgaa taactacggt ccttatcact ttccggaaaa attgattcca ctagtaattc
    25501 ttaatgctct ggaaggtaag gcattaccta tttatggtaa aggggatcaa attcgcgact
    25561 ggctgtatgt tgaagatcat gcacgtgcgt tatataccgt cgtaaccgaa ggtaaagcgg
    25621 gtgaaactta taacattggt ggacacaacg aaaagaaaaa catcgatgta gtgctcacta
    25681 tttgtgattt gttggatgag attgtaccga aagagaaatc ttaccgcgag caaattactt
    25741 atgttgccga tcgtccggga cacgatcgcc gttatgccat tgatgctgag aagattggtc
    25801 gcgaattggg atggaaacca caggaaacgt ttgagagcgg gattcgtaaa acaattaact
    25861 ggtatttgaa taataatgat tggtgccggc gagttcaaga tggctcttat caacgtgagc
    25921 gtttgggggt aattagatga aaggaattat tttagctggt ggttctggaa caaggcttta
    25981 tccaattaca atgggagttt caaagcagct attacccatt tatgataaac cgatgattta
    26041 ttatcctttg tcggtgctga tgttggcagg tatacaagat attcttatta ttacaacgcc
    26101 agaagatcag caagggttca tgagattact tggggatgga agccaatttg gtattaatct
    26161 gacatatgca attcaaccta gtccagatgg tttagcccaa gcatttatta ttggtgaaaa
    26221 ctttattgga aatgacacag tatgccttat gcttggagat aacatttttt ttggacaagg
    26281 ttttacaccc aaactccggg atgcagcaga aagaacatca ggtgccacag tctttggata
    26341 tcaagtaatg gatcctgaga gatttggagt agtggagttt gataggaatt ttaaagcttt
    26401 aagtattgaa gaaaaacctg ccaatccaaa atcaaattgg gctgtcacag gattatattt
    26461 ttatgacaat aacgttatcg atattgcaaa gtgtattaaa ccttctaaac gtggtgaatt
    26521 agaaattacg gcagttaatg aagtttatct cagaaataat aatttaactg ttgaattact
    26581 aggacgtggt tttgcatggc tggatacagg aacccatgac agcttgattg aagctgggag
    26641 ttttgtagag acagttcaaa aaaggcaagg gatgatggtt gcttgtccgg aggagattgc
    26701 atggcgaaat ggctggttaa accatgatga attacaggca aaaggtgaat ttttgaaaaa
    26761 aaatcactat ggacagtatt tactaaggtt aattaaatcg catgaataat aacgtaaaac
    26821 ttatacactt tgataagaaa ggtgacaacc gaggctttct tgttgttgtt gagcaaaata
    26881 aagatatccc aattgaaatt aaacgagttt actacatgtt tgatactggt tctgatgtaa
    26941 gacggggata tcacgcacat aaaaaactta cacaaatagc aatccctgtt aagggatctt
    27001 gcaagttttt gcttgatgac ggtaaatcgg tggaacatgt tacattagat tcaccgtcag
    27061 tgggattaat tatagaacca atgatttggc atgaaatgta cgattattca tcagattgtg
    27121 ttttaatggt gctggcagat gacttttatg atgaaagtga ttacattcgt aattatgctg
    27181 atttcatcag ggagataact aatgcgtaat ttgtcgctct ttacaaaaaa tacaaaatta
    27241 attccagttg aaataaatga tgcggaattt atttattcac tgcggataga ttct
//
LOCUS       DAZGCS010000068        26500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGCS010000068 DAZGCS010000000
VERSION     DAZGCS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.068"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..138
                     /locus_tag="ACHK01_002992"
     CDS             <1..138
                     /locus_tag="ACHK01_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO8097914.1"
                     /translation="YRWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTL
                     N"
     gene            212..580
                     /gene="cbeA"
                     /locus_tag="ACHK01_002993"
     CDS             212..580
                     /gene="cbeA"
                     /locus_tag="ACHK01_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO8097915.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            669..1042
                     /gene="cbtA"
                     /locus_tag="ACHK01_002994"
                     /pseudo
     CDS             669..1042
                     /gene="cbtA"
                     /locus_tag="ACHK01_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHK01_002995"
     CDS             1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHK01_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8097916.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1279..1359
                     /locus_tag="ACHK01_002996"
                     /pseudo
     CDS             1279..1359
                     /locus_tag="ACHK01_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1648..1794)
                     /locus_tag="ACHK01_002997"
                     /pseudo
     CDS             complement(1648..1794)
                     /locus_tag="ACHK01_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1848..2030
                     /locus_tag="ACHK01_002998"
     CDS             1848..2030
                     /locus_tag="ACHK01_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO8097917.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHK01_002999"
     CDS             complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHK01_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO8097918.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHK01_003000"
     CDS             complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHK01_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8097919.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHK01_003001"
     CDS             complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHK01_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO8097920.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCGTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHK01_003002"
     CDS             complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHK01_003002"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO8097921.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHK01_003003"
     CDS             5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHK01_003003"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO8097922.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHK01_003004"
     CDS             complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHK01_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO8097923.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7566..8624)
                     /gene="tsuA"
                     /locus_tag="ACHK01_003005"
     CDS             complement(7566..8624)
                     /gene="tsuA"
                     /locus_tag="ACHK01_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO8097924.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8803..10161)
                     /gene="plaP"
                     /locus_tag="ACHK01_003006"
     CDS             complement(8803..10161)
                     /gene="plaP"
                     /locus_tag="ACHK01_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO8097925.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(10151..10213)
                     /gene="yoeI"
                     /locus_tag="ACHK01_003007"
     CDS             complement(10151..10213)
                     /gene="yoeI"
                     /locus_tag="ACHK01_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO8097926.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(10428..11357)
                     /gene="yeeY"
                     /locus_tag="ACHK01_003008"
     CDS             complement(10428..11357)
                     /gene="yeeY"
                     /locus_tag="ACHK01_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8097927.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(11403..12227)
                     /gene="yeeZ"
                     /locus_tag="ACHK01_003009"
     CDS             complement(11403..12227)
                     /gene="yeeZ"
                     /locus_tag="ACHK01_003009"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8097928.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(12310..12564)
                     /gene="yoeB"
                     /locus_tag="ACHK01_003010"
     CDS             complement(12310..12564)
                     /gene="yoeB"
                     /locus_tag="ACHK01_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO8097929.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(12561..12812)
                     /gene="yefM"
                     /locus_tag="ACHK01_003011"
     CDS             complement(12561..12812)
                     /gene="yefM"
                     /locus_tag="ACHK01_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO8097930.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            13095..13145
                     /gene="hisL"
                     /locus_tag="ACHK01_003012"
     CDS             13095..13145
                     /gene="hisL"
                     /locus_tag="ACHK01_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO8097931.1"
                     /translation="MIRVQFKHHHHHHHPD"
     misc_feature    13121..13244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13291..14190
                     /gene="hisG"
                     /locus_tag="ACHK01_003013"
     CDS             13291..14190
                     /gene="hisG"
                     /locus_tag="ACHK01_003013"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO8097932.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            14196..15500
                     /gene="hisD"
                     /locus_tag="ACHK01_003014"
     CDS             14196..15500
                     /gene="hisD"
                     /locus_tag="ACHK01_003014"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO8097933.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNVETFHTAQ
                     KLPPIDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNIGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            15497..16567
                     /gene="hisC"
                     /locus_tag="ACHK01_003015"
     CDS             15497..16567
                     /gene="hisC"
                     /locus_tag="ACHK01_003015"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO8097934.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDS
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            16567..17634
                     /gene="hisB"
                     /locus_tag="ACHK01_003016"
     CDS             16567..17634
                     /gene="hisB"
                     /locus_tag="ACHK01_003016"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO8097935.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            17634..18224
                     /gene="hisH"
                     /locus_tag="ACHK01_003017"
     CDS             17634..18224
                     /gene="hisH"
                     /locus_tag="ACHK01_003017"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO8097936.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            18224..18961
                     /gene="hisA"
                     /locus_tag="ACHK01_003018"
     CDS             18224..18961
                     /gene="hisA"
                     /locus_tag="ACHK01_003018"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO8097937.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNV"
     gene            18943..19719
                     /gene="hisF"
                     /locus_tag="ACHK01_003019"
     CDS             18943..19719
                     /gene="hisF"
                     /locus_tag="ACHK01_003019"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO8097938.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            19713..20324
                     /gene="hisIE"
                     /locus_tag="ACHK01_003020"
     CDS             19713..20324
                     /gene="hisIE"
                     /locus_tag="ACHK01_003020"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO8097939.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(20420..21397)
                     /gene="wzzB"
                     /locus_tag="ACHK01_003021"
     CDS             complement(20420..21397)
                     /gene="wzzB"
                     /locus_tag="ACHK01_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO8097940.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIAQRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YLYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(21544..22710)
                     /gene="ugd"
                     /locus_tag="ACHK01_003022"
     CDS             complement(21544..22710)
                     /gene="ugd"
                     /locus_tag="ACHK01_003022"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO8097941.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRYADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(22958..24364)
                     /gene="gndA"
                     /locus_tag="ACHK01_003023"
     CDS             complement(22958..24364)
                     /gene="gndA"
                     /locus_tag="ACHK01_003023"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO8097942.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(24470..25498)
                     /gene="galE"
                     /locus_tag="ACHK01_003024"
     CDS             complement(24470..25498)
                     /gene="galE"
                     /locus_tag="ACHK01_003024"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO8097943.1"
                     /translation="MAILVTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRV
                     KEITGSDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTEYYDNNVY
                     GTLVLIEEMISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLMVENIL
                     HDVSLAYPFFRTTILRYFNPVGAHPSGKIGEDPNGIPNNLMPYICSVASGKYKQLSIY
                     GNDYETKDGTGVRDFIHVMDLANGHIAALKHRDEGSNYKVYNLGTGHGYSVLELLAAF
                     QRITSVNIPYVLVERRAGDIAECWSDPSKAYLELGWKACLGLDEMVRDAWNWQQSNPD
                     GYKFSEKN"
     gene            complement(25577..>26500)
                     /locus_tag="ACHK01_003025"
     CDS             complement(25577..>26500)
                     /locus_tag="ACHK01_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8097944.1"
                     /translation="SDFFNTFFLARKIKEINPDSVLCFFPKPVIYGTLAAMIAGVKKR
                     YALLEGLGFCFTQNKDKDSFKKIVLRKIQGFLYKMTLPFATKVLFLNNDDYHDLVVKN
                     NIKIKDYKVIGGIGVNLNEFSYHPPICGEIHFGMVARLLREKGVCEFVEAAKIIKKKY
                     PMVRFSIAGDFDDNPGGIDAAQIVNWKAEGYVEFLGPCSYIKEYLSDISVFVLPSYRE
                     GVPKSTQEAMAIGRAIITTDVPGCRETVIDGYNGYLVPPWDTQKLALVMETFIHEREL
                     IVTMGLNSRRFALSKFNESESANSLVNIITA"
BASE COUNT         7007 a         6696 c         6292 g         6505 t
ORIGIN      
        1 taccgctggc acggcagtgc tgaagcgtat accggtcgtg aagtgcagga tattcccggt
       61 gtgctggccg tgtttgctga acgccgtaag gacagttttg gtccgtatgt ccggctgatg
      121 agcgtcaccc tgaactgagt gggaattctg atgagcagaa ttatcgccac taccggacca
      181 ttcttagccg attttctgta aggattttat cgtgtcagac acactccccg ggacaacact
      241 tcccgacgac aatcacgacc gcccctggtg ggggctgccc tgcaccgtga cgccctgttt
      301 cggggcacgt ctggtgcagg agggtaaccg gttgcattac cttgccgacc gcgccggtat
      361 cagaggcctg ttcagcgatg cagatgcgta ccacctggac caggcctttc cgctgctgat
      421 gaaacaactg gaactcatgc tcaccagcgg tgaactgaat ccccgccatc agcataccgt
      481 cacgctgtat gcaaaagggc tgacctgcaa agccgatacc ctcagcagtt gtggttacgt
      541 ttatctggct gtttatccga cgcccgaaat gaaaaattaa ctctccagaa tagccttctg
      601 ctacggcctg gtgttttcac cacgccactt ttccattttt atatctgcat atcaggaaaa
      661 tcttcagtat gaaaacatta cctgtattac ccgggcaggc gaccagttct cacccgtctc
      721 ctgttgaaat ctggcagata ctgctgtccc gactgctgga ccagcactat ggcctcacac
      781 tgaatgacac accttttgcc gatgaacgtg tgattgagca gcatattgag gcaggcattt
      841 cactgtgtga tgcggtgaac tttctcgtgg aaaaatacgc gctggtgcta ccgaccagcc
      901 gggattcagc gcctgtaccc gctctcagtt aataaacagc atcgatatcc tccgggctcg
      961 cagggcgacc ggcctgatga cccgcgacaa ttacagaacg gtaaataaca ttaccctggg
     1021 taagtatccg gaggcgaaat gatgacactg gaagccgaca gcgttaacgt acaggcgctg
     1081 gatatggggc acattgtcgt tgacattgat ggtgttaata tcactgaact gattaataag
     1141 gccgctgaaa aaggttattc actccgcgtg gtggatgacc gtgactctac cgaaacaccg
     1201 gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgatgca gggtatagtg
     1261 ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga agatgtcctt
     1321 accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa caggcttcat
     1381 ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga aaggaaaacc
     1441 gataataatg gaatggacgc cccgaccatg aagcaacggt aggatattac tgataaacct
     1501 tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca aaaaatgaat
     1561 tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg gattaaagta
     1621 agcgtcaacg gagcaccgta ttgacgctta tttattggtg agaactacgt tccatggcag
     1681 gagttcgtca acacggttgg agggccattc cggcagtatg ctcagaatat ggcgcagata
     1741 cgcttccgga tcgataccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa
     1801 tctcagcgct gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg
     1861 tcattggagt agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa
     1921 gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcagtt aataacaatg
     1981 gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgttaa tttcgattgg
     2041 ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca atgagggttg
     2101 ccgggcaacc ctcattgaat aaaacgggaa ttacttcgct tcgccgtttt tcatttcgcc
     2161 catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg catttttgat
     2221 gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga tgccctggat
     2281 tgcttcaacg ctcatccctg gcttgatgta atcgctcagg ttgtccagca acaggacgcg
     2341 cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca gtttgttctt
     2401 acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg tagtttccat
     2461 ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat tcgttagggc
     2521 caacattact catagctgcc gcagatgaca atgcttttat cccttttcac tatcataccc
     2581 tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga attatttgcg
     2641 caaggcccgg caaatcagat tcgatagcaa ctcaagctga tgtgccgttt ccatgttcag
     2701 ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt tattcagcaa
     2761 ctgacgcagc tcttccacag catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg
     2821 attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca tatgctgggt
     2881 atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg cccagtacgc
     2941 attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga taccttcata
     3001 tatcgatttt ggaatacggg tttctttgct ggcgggcgca atcagcccac gcattttcac
     3061 ggcatcggtc aagagttttt gtagatggct ttccagacgt gggcgttcga gtaagttcgg
     3121 tgagaatgca gattgataga cccgattata ctcggtcaga cttttcgcca gttgaatgcg
     3181 ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca gtaaagagcc
     3241 gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg gggaaccaac
     3301 aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc ccagcgccag
     3361 ccaaccgcaa agaaacatcg ccgccgcgca ccagactaac atcagcggta acgaaattaa
     3421 ctccagttgc agagcgataa gacccaaaat cgaacccaac accgtaccgc caatacgttc
     3481 gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca ccaccatggt
     3541 gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa aagtgagcag
     3601 gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata cccggatttc
     3661 aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag attaaaagcg
     3721 ctcataactc atctgtcggc cagaaggcgc gcctctgaat tatccgacgg acataccggg
     3781 aacagtgtaa tttcccggca ggagcgaaaa atttgaaagg cgcatcttac tcttttcgct
     3841 ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta gtgatgtttt
     3901 ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc attgttcaag
     3961 taaacctcga agcagggctt tggtaacatt tcataagcac tgtcctgcaa gaggctatta
     4021 aagaactgat accagggttt agcaaaatca tcaccgacta cacgagctac cgccaccgca
     4081 tactgaccac ctgaaatttc tgtcagaatg acgccctcac tgttttcggg aagcgtaaag
     4141 taacccggca ccgtcacgac ggtgccgcag cgtaattttt cggcgggtgt ttcatctgga
     4201 ttgtcgtaat agatagcaac ccactccttc ggcacaatat ttttactatc tacccacatc
     4261 atcaactgct caaaaccttt ctttaccgtc tgttcccagg ggccaacgag atggaaacct
     4321 gcaacggtac gtttatcttc ctgcttaatc tcgtagttca tgacgcctcc attaatactg
     4381 tttttatata cagtatagtt gcaatattaa aaccacaagg aatgagtgtt gattatgcga
     4441 gcagactcgc actcctgcca gtctgctgca aaagaaaggt caggccttat ggtggaaata
     4501 atcactcagg cgagaaaaca tgctgccttc cccgacagat tccagggtaa ccagcggcca
     4561 gtgcgccacc tgtttatcac ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct
     4621 aattggcgcg gtgagttctt taccatcaag ggtatatttg gctttgatat gtggaatttc
     4681 ggctttcggt agcaccatcc agaactcttg ttccgttccc aggtcgatat tttctttatc
     4741 gccataccag atgcgttccg ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt
     4801 aaagttttgt tgcccccaac gcagtaattt tcttgcctct tcctcacgac cttttgcact
     4861 gtcagccccc attatcactg caatgagacg atgctgccca tctacagccg aagcaatgag
     4921 attaaacccg gcaccagaag tatgacccgt tttcaggccg tcaacattca tggttttatc
     4981 ccacaataat ccgttacggt tttgctgggt gataccgttc caggtgagac ttttctcact
     5041 gtacatatga taaaactcgg gctcgccgtg gatgatagca cgagaaagca cagctaaatc
     5101 ataagccgag ctatgctggc caggtgcatc cagaccatgc actgtttcaa aatgcgtatc
     5161 cttgagatgc agcttctcgg catagttgtt catcatttca acaaactgcc gttgcccacc
     5221 ggcaatatag tcagccagag caacacaagc gtcatttccg gaatccacaa ttaaaccacg
     5281 gcttaaatca cgtaccgata cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac
     5341 aaacaccgga ttatctttcg cccacgcatc gcgccccacg gtgacaatat cgtctggcgt
     5401 aatgcgatga ctatcgatag cgcgatccac gacataaccc gtcatcagct ttgtcaggct
     5461 ggcgggattg cgctgttgat gctcattacc cgcggtgagg atctgaccgg tggtgtaatc
     5521 catcaatacc caggacccgg catgaatctc tggaggctga ggtgaaaaag gaatgttttc
     5581 cgccgcaaaa ccagacgata agttaaaaac gaacaaagaa gcagcaataa taagacggcg
     5641 tttcaacagc aaaccctcag gagtttcaaa tagctgttct ttttacggaa atacttatga
     5701 actggctgga ataaagtgca agaaaatgtg actaccctct catttttatc tgacatgatc
     5761 tgttgccact cgctgccaaa ttgtggcgct aaagctgatt agcacggtga tatttgatac
     5821 tctggcagac agcagaaata acggatttaa cctaatgatg aatgacggta agcaacaatc
     5881 tacctttttg tttcacgatt acgaaacctt tggcacgcac cccgcgttag atcgccctgc
     5941 acagttcgca gccattcgca ccgatagcga attcaatgtc atcggcgaac ccgaagtctt
     6001 ttactgcaag cccgcggatg actatttacc ccagcctgga gcagtattaa ttaccggtat
     6061 taccccgcag gaagcacggg cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca
     6121 ctcgcttttt accgtaccga agacctgtat tctgggctac aacaatgtgc gtttcgacga
     6181 cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat ccttacgcct ggagctggca
     6241 gcatgataac tcgcgctggg atttgttgga tgttatgcgt gcctgttatg ccctgcgccc
     6301 ggaaggaata aactggcctg aaaatgatga cggtctaccg agctttcgcc ttgagcattt
     6361 aaccaaagcg aatggtattg aacatagcaa cgcccacgat gcgatggctg atgtgtacgc
     6421 cactattgcg atggcaaagc tggtaaaaac gcgtcagcca cgcctgtttg attatctctt
     6481 tacccatcgt aataaacaca aactgatggc gttgattgat gttccgcaga tgaaacccct
     6541 ggtgcacgtt tccggaatgt ttggagcatg gcgcggcaat accagctggg tggcaccgct
     6601 ggcgtggcat cctgaaaatc gcaatgccgt aattatggtg gatttggcag gagacatttc
     6661 gccattactg gaactggata gcgacacatt gcgcgagcgt ttatataccg caaaaaccga
     6721 tcttggcgat aacgccgccg ttccggttaa gctggtgcac atcaataaat gtccggtgct
     6781 ggcccaggcg aatacgctac gcccggaaga tgccgaccga ctgggaatta atcgtcagca
     6841 ttgcctcgat aacctgaaaa ttctgcgtga aaatccgcaa gtgcgtgaaa aagtggtggc
     6901 gatattcgcg gaagccgaac cgtttacgcc ttcagataac gtggatgcac agctttataa
     6961 cggctttttc agtgacgccg atcgcgcagc aatgaaaatt gtgctggaaa ccgagccgcg
     7021 taatttaccg gcactggata tcacttttgt tgataaacga attgaaaaac tgttgttcaa
     7081 ttatcgggca cgcaacttcc cggggacgct ggattatgcc gagcagcaac gctggctgga
     7141 gcaccgccgc caggtcttca cgcccgagtt tttacaaggt tatgcagatg aattgcagat
     7201 gctggcacaa caatatgccg ataacaaaga aaaagtggcg ctgttaaaag cgctttggca
     7261 gtacgcggaa gagatcgtct agtttgaagc ctccgccgct gatacggcgg aggacaatgt
     7321 tgggtcaggc tttttgaacg gtgatgctcc acgcagcatc gccaatctgc tgataatcgg
     7381 tgatggcatg tccttcttct gccgcccatt gtgggattgc ttctgttgcc tgggtgcaat
     7441 caaactcaat taccaactca tcgccgctgg ccattcctgc cagtgctgct tttgcttcta
     7501 tgagtggaaa cggacagacc tgcgtcacca catccagctt tttaatcgcc atctttacac
     7561 cttacttaat ttgccgcagc agttgccagt cgcgccttac gctgcggtcg aacatacaca
     7621 agccaggacg cagtccagac tccgagaatc ataaatacaa ggccaatcca gccctgccag
     7681 gtcatcattg cagtcataac cagtccatta ccgatagagc aaccacctgc aatactggca
     7741 ccgaagccca tcagtacacc acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg
     7801 cgaacgcgga actcacggct cgctttggct gcaataaatg accccacgaa gatccctaac
     7861 accaggaaaa cgccccagtt aatgtatttc acgtcacccg cgaccagaaa ttgcagaata
     7921 ttggccgttg gggaagtgat tcctaaccca aacatgcgtc cggttgcttc actcaggggc
     7981 caggctaaaa gcgcaatcaa accgatgagt acagcagtga caaagggatg ccagcgtttt
     8041 tcaaacagaa tatgagcgat cccggttcgg cgcggcggta aggtcgcgac tttgagtttt
     8101 ggcttcttca actctttcat caccacccag agcgttatca ccagcaaaac ggcaaccaac
     8161 ggccacacag acaaattaaa agtctcagca atagagttat gttcagtact gtagtgctgc
     8221 aaggtttgat ttaaaccact ggcatgtgga gaacgcatca ccgcactcat caccatataa
     8281 gtgaaaagcg cgatccaact gccgatcaat ccctcacccg cgcgatacca ggtcccggtg
     8341 gcacatccac ccgccagaac aattcccagc ccaaagatat acccacctat aacagtacca
     8401 agccacggga acgccccggc ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc
     8461 cccacacttt gtaccgaaat tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg
     8521 agatacatat cgcgaaaacc acctgtcagg caaaaacgcc cacgctgcat gacaaatccc
     8581 agcagagcac cacaaattag cccgcttaat atcattgaaa acataaatta attaaccaga
     8641 tgaatgttaa tagggaaatt attcatgact ggtggaatgc agaccaataa ccaaattctc
     8701 taaattagaa caaatggtta ttaatgagga tgtcgataaa aaagaataaa aaaaccggag
     8761 ccaaatattc attcgactcc ggtttattat tacaaaagat gattacgcta cgtcttcgta
     8821 ctgcggaacc gggttacgga aactcttggt gacgcaagcc aggtaaatca gaccgatagc
     8881 cgcccagatc agacccagaa ccattgagct ttcttccagg ttaacccaca gcgcaccaac
     8941 ggtcagcgca ccacacatcg gcaggaacag atactggaag tgatctttca gcgtcttatt
     9001 acgcttctca cggatccaga actgcgagat gaccgacagg ttaacgaagg tgaacgccac
     9061 caacgcacca aagttaatca gcgctgtagc cattaccagg tcgaagttga ttgccagcag
     9121 ggcaatcgcg ccaaccagga tgatgttcat cgccggagta cgccatttcg ggtgtacata
     9181 accgaagaag cttttcggga atacgccgtc acgacccatt acgtacatca gacgcgctac
     9241 gcctgcatgc gccgccatac cggacgccag tacggtaatg gtggagaaga tcagcgcgcc
     9301 aacctggaac gctttacccg caacgtacag catgatttca ggctgtgacg catccggatc
     9361 tttaaagcga gagatatccg ggaagtacag ctgcaggaag taagttgcaa agatgaagat
     9421 catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt
     9481 ggtttcttcc gacaggttgc tgatgccgtc aaagccggta aaggagaagc acaggattgt
     9541 cgccccggta atcatcggga taacatgcgc atcgccagac cagaacggac gagtgctcgc
     9601 cagcgtacca gcgccttcac cttcaaatac gccataaaca accatgccca gaatcaccgc
     9661 gatcagcacc acctgcaaca cgacgattac agtgttgaag ttcgctacgg atttcagact
     9721 acgcaggtta aaggcggtca tgaaggccac caacgccacc acaaacatcc acgatgggat
     9781 ggaaggcacc agagcttcaa aatagatttt cgccagcaga atgttgatca tcggcgcgaa
     9841 cagatagtcg agcagagaag accaacccac cataaagccg acagtcgggc taatggattt
     9901 ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg cgaaccagct tcccgtagct
     9961 cagagccgta aacaggatcg caatcaatgc gaacgcatag gctgtcggca catgaccatc
    10021 cgtgaggcct gaaacgatac caaaagtatc aaacagcgtc atcggctgca tataggcaag
    10081 acccatcatt acaaccggaa ctaacgtaag cgttttacgc aattccacgc gagaggtgtt
    10141 tggagtaacg ttatgcgaca tggtcattct cctttacggt gataaccgtc gcgtaagcaa
    10201 aaaattgccc catttttttg gattcctcag cgacaacaac tgtcgatttt tagtaaatat
    10261 ctatccggta cgaagcccgg cctcttggta tgaatgattg gtttgaagca aaaaataacc
    10321 gacgctgatg aaacgtcggt ttttagtcat tttttgacag cggcgcattg tgcctaaatg
    10381 ggggggaaat gacaagagaa tgagaggctt gtcataataa tttttcttta aatggctgat
    10441 tttccgtcat cagattttgc ataaacaccg gcgatcgcac tatttgctaa aatttcatcc
    10501 cgccaccagc cactggcaag tcctgcggtc tgttcattcc agccaatcca gacgggttca
    10561 aagagagttt gggcgaccac tttcttctcg attaacgcac cactatcgag aaaacgttgc
    10621 gccagataac ggggtaaata gccgcagccg aggccgctaa tttgcagctc cagcttggtt
    10681 ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg
    10741 cctgcctgcg cagtatcccc caccacaatc gcccgatatc gcttaatgat acgacggttt
    10801 aacggttctt cttccagggc taacggatga tgaggcgcta ccgcaaagac ttgttccaga
    10861 tcgcccaacc gcgagaaacc aaactcactg gaagatggtg gctcatgcat cgcgccaaca
    10921 ataatatccg ctcgcccctg agtcagggca tcccatgaac caccgagtac gccattgata
    10981 aacttcaggc gcgtcacgct atgatgttga taaaaagctt caatcagtgg cgcaagcaga
    11041 gaaaaaggaa acgtgtcgtc cacgccaatc accagttcgt tttcccagcc ttcatgcagt
    11101 ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa cttcccgccc tttctctaat
    11161 aacatttttc cggtgcgggt gaatttagcg cggtgtccgc tacgatcaag caattgaata
    11221 ttaaggtcgc tttccagctt gtgaacggtg taactcagag cggacggcgt cttatagagt
    11281 ttggccgacg ccgccgcaaa actgccttct ttttctaagg catcgaggat catcaacaca
    11341 tccagcagtg gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc
    11401 gtttactcca gcggcattac cagcggatcg ggatactggt attcaaatcc cagttcatta
    11461 caaatccgac tgccatcaat aatcttacct ttgccgctgt ccagactatt tctgaactgc
    11521 ggaggctcca gccccagtaa acgggccatc tgcggataga aaacattacg cgcagggtga
    11581 gctggcgcac atatattata gatgtgtccg cctttaggtg cctgtaacaa cagagtgata
    11641 gcgccaataa catcttctaa atggactaaa ttaacaccat gttcaccatc aggcgcggtt
    11701 tttccggcaa agaagcgtcc gggatgacgt cccggtccga ccagccccgc aagacgcaga
    11761 atatcgaccg aagtaccggg taaattgtgc agccagtctt cgagttcttc taatactcgt
    11821 ccactgttgg tcaccggatt acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca
    11881 tagacagacg ttgagctggt aaaaataatt cggggaatac gatgggccag cgcgctatcc
    11941 actaactctt gtaccgcttg taaatagaac tcatcgccgg ggccgctacg acgtgccgga
    12001 agcgtaatga ccagcgcatc ggcatccatc agggcatcca gatcgtcaga atcgcaaact
    12061 aactcaggct ccatgcgaag cagatagctg tcaatgccac tcattcgggc cgcttcgaca
    12121 ccatcttgtg tggttttact cccggtgact tgccagcctc gcgctgaaag tgacatcgcc
    12181 agcggcatgc ctaaccaccc taacccgaca attgcgacct ttttcatccg ttatctcctg
    12241 atacacctta gatctataag gctacgctag cgtatcaaaa ctgacaattc attctatgaa
    12301 tgaatctgtt caataatgat aacgacatgc tgcaatgagc agtgaatcgt cggtaaccgc
    12361 gtataccaga cggtgctcct ctgtaatgcg tcgggaccag aaacctgaca aattatgttt
    12421 caggggttct ggcttcccct taccttcaaa tggcgttctg cgggtatctt tgataagttc
    12481 attgatcttt ttaacaattc gcttatctgt ttcctgccag tacagataat catcccatga
    12541 ttcctcagac cagattagtt tcactcaata atgtcctttt ctgttccttt gcctgatttc
    12601 aggctatcga ttgagtccat caatctccgg gcgttagcgg gggaacgcag tagataagcc
    12661 gtctcttcca gcgagttgta ttcttcgagt gacatcagaa cacaagcctc tccattctga
    12721 cgagtaataa ggatcggggc atgatcttca acggctttca tcattgttgc cgacaaattc
    12781 tgacgcgctt cgctgtagct aattgtacgc atgtcaatct cctcttttgt acagttcatt
    12841 gtacaatgat gggcgttaat taactattta ttaattagtt tgtagatcaa ggtattgtca
    12901 gtgagacgaa aatccaggct tcgctatttt tggtgccgtc agcttagagg acagtcctct
    12961 tagccccctc ctttccccgc tcattcatta aacaaatcca tcgccataaa atatataaaa
    13021 aagcccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat
    13081 tcacagagac ttttatgata cgcgttcaat ttaaacacca ccatcatcac catcatcctg
    13141 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg
    13201 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg
    13261 gttcagacag gtttaaagag gaataacaaa atgacagaca acactcgttt acgcatagct
    13321 atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tgctggcgcg ctgtggcatt
    13381 aaaattaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt
    13441 ctgcgcgtgc gtgacgacga cattcccggt ctggtaatgg atggcgtggt agaccttggg
    13501 attattggcg aaaacgtgct ggaagaagag ctgcttaacc gccgcgccca gggtgaagat
    13561 ccacgctact ttaccctgcg tcgtctggat ttcggcggct gccgtctttc gctggcaacg
    13621 ccagttgatg aagcctggga cggcccgctc tccttaaacg gtaaacgtat cgccacctct
    13681 tatcctcacc tgctcaagcg ttatctcgac cagaaaggca tctcttttaa atcctgctta
    13741 ctgaacggtt ctgttgaagt cgccccgcgt gccggactgg cggatgcgat ttgcgatctg
    13801 gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt tatctatcgc
    13861 tcgaaagcct gcctgattca acgcgatggc gaaatggaag aatccaaaca gcaactgatc
    13921 gacaaactgc tgacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg
    13981 atgcacgcac cgaccgaacg tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
    14041 ccaactattc tgccgctggc gggtgaccaa cagcgcgtag cgatgcacat ggtcagcagc
    14101 gaaaccctgt tctgggaaac catggaaaaa ctgaaagcgc tgggtgccag ttcaattctg
    14161 gtgctgccga ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact
    14221 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
    14281 ctgaaagcat tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggcgatg
    14341 aggccctgcg ggaatacagc gcgaagtttg ataaaaccac ggttaccgcg ctgaaggtgt
    14401 ctgctgagga gatcgccgcc gccagcgaac gcctgagcga tgaactaaaa caggcgatgg
    14461 cggtggcagt taagaatgtt gaaaccttcc acactgcgca aaaactgccg ccgatagatg
    14521 tagaaacgca gccaggcgtg cgttgccagc aggtcacgcg cccggtcgct tcagttgggt
    14581 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcgactccgg
    14641 cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga
    14701 tcctttatgc ggcgcagctg tgcggtgtgc aggacgtgtt taacatcggc ggcgcacagg
    14761 ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc
    14821 cgggtaacgc ctttgtcacc gaagcaaaac gccaggtaag ccagcgtctg gacggtgcgg
    14881 cgatcgatat gcccgcaggc ccgtcggaag tgctggtgat tgctgacagc ggcgctacgc
    14941 cggatttcgt ggcttctgat ttgctttctc aggctgaaca cggcccggac tcacaggtga
    15001 ttttactgac gcccgatact gatatggcgc atcaagttgc cgaggccgtc gaacgccaac
    15061 tggcagaact gccgcgtgcc gaaaccgccc gccaggcact gagcgccagc cgcctgatcg
    15121 tgactaaaga tttagcgcag tgcgtagaga tctccaacca gtacggcccg gagcacctga
    15181 tcattcagac ccgcaacgcc cgcgaactgg tcgatggcat caccagcgcc ggttcggtat
    15241 ttcttggtga ctggtcaccg gaatcggcag gcgactatgc ctccggcact aaccatgttc
    15301 taccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc
    15361 gcatgaccgt acaggaactg tcgaaagagg ggttctccgc gctggcttca accatagaaa
    15421 cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc cgttactttg cgtgttaacg
    15481 cccttaagga gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg
    15541 caacctgacg ccgtatcagt cggcgcgtcg tctgggcggt aatggcgacg tctggctgaa
    15601 cgccaacgaa tatcccacag ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
    15661 cccggaatgt cagccgaaag cggtgattga aaattacgcg caatatgcag gcgtaaaacc
    15721 ggaacaggtg ctggtcagcc gtggcgcgga cgaaggtatt gaactgctga tccgcgcttt
    15781 ttgcgaaccg ggtaaagact ccatcctcta ctgcccacca acgtacggca tgtacagcgt
    15841 cagcgccgaa acgattggcg tcgagtgccg cacagtgccg acgctggaca actggcaact
    15901 ggacttacag ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc
    15961 caacaacccg accgggcaac tgatcaatcc gcaggatttt cgcaccctgc tggagttaac
    16021 ccgcggtaag gcgattgtgg ttgccgatga agcctatata gagttttgtc cacaggcatc
    16081 gctggctggc tggctggcgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc
    16141 ttttgctctg gcgggccttc gttgcgggtt tacgctggca aacgaagaag tcatcaacct
    16201 gctgatgaaa gtgatcgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca
    16261 ggcgttaagc ccgcagggaa tcgtcgccat gcgcgaacgg gtagcgcaaa ttattgcaga
    16321 acgcgaatac ctgattgccg cactgaaaga gatcccctgc gtggagcagg tttttgactc
    16381 tgaaaccaac tacattctgg cgcgctttaa agcctccagt gcagtgttta aatctttgtg
    16441 ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
    16501 aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
    16561 agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
    16621 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaaccggg cgtgatcccg
    16681 gaactgctga agctgcaaaa agcgggctac aagctggtga tgatcactaa tcaggatggt
    16741 ctgggaacac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag
    16801 atcttcacct cgcaaggcgt acagtttgat gaagtgctga tttgtccgca cctgcccgcc
    16861 gatgagtgcg actgccgtaa gccgaaagta aaactggtgg aacgttatct ggctgagcaa
    16921 gcgatggatc gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg
    16981 gaaaacatgg gcattaatgg tttacgctac gaccgcgaaa tcctgaactg gccaatgatt
    17041 ggcgagcagc tcactaaacg agaccgttac gctcacgtag tgcgcaacac caaagagacg
    17101 cagattgacg ttcaggtgtg gctggatcgc gaaggtggca gcaagattaa caccggtgtt
    17161 ggcttctttg atcacatgct ggatcagatc gccacccacg gcggtttccg tatggaaatc
    17221 aacgtcaaag gcgacctcta tatcgacgat caccacaccg tcgaagatac cggcctggcg
    17281 ctgggtgaag cgttaaaaat tgcccttggc gacaaacgcg gtatttgccg ctttggtttt
    17341 gtgctaccga tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac
    17401 ctggaatata aagccgagtt tacctaccag cgcgtgggcg atctcagcac cgaaatgatc
    17461 gagcacttct tccgttcgct ctcatacacc atgggcgtga ccctgcacct gaaaaccaaa
    17521 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cttttggtcg caccctgcgc
    17581 caggccatcc gcgtggaagg cgataccctg ccctcgtcga aaggagtgct gtaatgaacg
    17641 tggtgatcct tgatactggc tgcgccaacc tgaactcggt gaagtctgcc attgcgcgcc
    17701 acggttatga acccaaagtc agccgtgacc cggacgtagt gttgctggcc gataaactat
    17761 ttttacccgg cgtaggcacc gcacaagcag cgatggatca ggtgcgtgag cgcgaactgt
    17821 ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg atgcaactgc
    17881 ttgggcggcg cagcgaagag agcaacggcg tcgatttgct gggcatcatc gacgaagacg
    17941 tgccgaaaat gaccgacttt ggtctaccgc tgccccatat gggctggaac cgcgtttacc
    18001 cgcaggcagg caaccgcctg tttcagggaa ttgaagacgg cgcgtacttt tactttgttc
    18061 acagctacgc aatgccggtt aatccgtgga ccatcgccca gtgtaattac ggcgaaccgt
    18121 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
    18181 gtgccgctgg cgctaagctg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
    18241 agatttaatc gacggcactg tggtgcgtct ccatcagggt gattatggca aacagcgcga
    18301 ttacggtaac gacccgctgc cgcgcttaca ggattacgcc gcgcagggtg ccgaagtgct
    18361 gcacctggtg gatctgaccg gggcaaaaga tccggctaaa cgtcaaatcc cgctgattaa
    18421 aactctggtg gcgggcgtta acgttccggt gcaggttggc ggcggcgtgc gtagcgaaga
    18481 agacgtggcg gcgttactgg aagcgggcgt tgcgcgcgtg gtggtcggct ccaccgcggt
    18541 gaaatcacca gaaatggtga aaggctggtt tgaacgtttc ggtgccgatg ccttagtgct
    18601 ggcgctggat gtccgtattg acgagcaagg caacaagcag gtggcggtca gcggctggca
    18661 agagaattcg ggtgtgtcgc tggaacaact ggtggaaacc tatttgcctg tcggcctgaa
    18721 acatgtgctg tgtaccgata tctcgcgcga cggcacgctg gcaggttcta atgtctcttt
    18781 atatgaagaa gtgtgcgcca gatatccgca ggtggcattt cagtcatccg gcggcattgg
    18841 cgacattaac gatgttgctg ccctgcgtgg tactggcgtg cgcggcgtaa tagttggtcg
    18901 ggcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgtata
    18961 attccgtgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
    19021 gaaatcattg gcgatatcgt gccgctggca aaacgctacg ccgaagaagg tgcagacgaa
    19081 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
    19141 tctcgcgtgg cggaagtgat cgacattccg ttctgtgtgg cgggtgggat taagtctctg
    19201 gaagatgccg cgaaaattct ttcctttggc gcggataaaa tttccatcaa ctcgcctgcg
    19261 ctggcggacc cgacgttaat tactcgcctg gccgatcgct ttggcgtgca gtgtattgtg
    19321 gtgggtattg atacctggta cgacgctgaa accggtaaat atcatgtgaa tcaatatact
    19381 ggcgatgaaa gccgcacccg cgtcactcag tgggaaacgc tcgactgggt acaggaagtg
    19441 caaaaacgcg gtgccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac
    19501 ggttacgacc tcgaacaact gaaaaaagtg cgtgaagttt gccacgtccc gctaattgcc
    19561 tccggtggcg cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac
    19621 ggcgcgctgg cggcttccgt attccacaaa caaataatca atattggtga attaaaagcg
    19681 tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa cgtcgcgaac
    19741 tggactggga aaaaaccgac ggacttatgc cggtgattgt gcagcacgcg gtatccggtg
    19801 aagtgttaat gctgggctat atgaacccgg aagccttaga taaaaccatc gaaagcggca
    19861 aagtcacctt cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca
    19921 atttcctcaa cgtagtaaat attgcgccgg actgcgacaa cgacacgcta ctggtactag
    19981 cgaatcctat cggaccaacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc
    20041 accagtggct gttcctgtat caactggaac aactgctcgc cgaacgcaaa tctgccgacc
    20101 cggaaacctc ttacactgcc aaactgtatg ccagcggcac caaacgcatt gcgcagaaag
    20161 taggtgaaga aggcgtggaa accgcactgg cagcgacggt acatgaccgc tttgagctga
    20221 ccaacgaggc gtctgatttg atgtatcacc tgctggtgct gttgcaggat caggatctgg
    20281 acttaacgac ggtgattgag aacctgcgta aacggcatca gtaagttgcg ggtgatcgga
    20341 tgcgatgttg ttgtcgcatc cggcaaaaaa aacgggcaag gtgtcaccac cctgcccttt
    20401 ttctttaaaa ccgaaaagat tacttcgcgt tgtaattacg cagagcgtta cgccccagca
    20461 caatccccgc accaaccatg ccgcccagca gtaccgccag aatcaaagtg attgcctttt
    20521 tcgggctatc gcgacgaata ggtaacgtcg gtttcatcac atagaggtaa gcatgaatat
    20581 caagatcatc aacttttaag ctttcgatat ccagcaggtt ttgacgtgtc tgatagtagt
    20641 ttggtgagaa caccaacgga cgagtcgctt catgcttaat catcgactcc agcgcttcgc
    20701 tccccaaaag gaacaacgta tcttgtgtca catcttcagt ctgctgaacc tgtggctttg
    20761 tcacctgcgc ctgattcgca tactgcaacg cttcctgaat ctgacggata cgcagatctt
    20821 tctgctcctg cgcaactact tcctgagttt tcagagaatc ctgcaagttt ttcatacgct
    20881 gcgcgatgtt gtctttcagg tcttttccca gttcttcatt tacctggtcg tcaacttgct
    20941 ggatatactt cgccagctgc ttctgagcac cttcaggtgt ttcaccaaca taagacaccg
    21001 ctaaaggtaa tgactgattt ttgacggtag gctcaatggt cagcttttct gcttcttcct
    21061 ggttatccag cgtttccgct aatgctgaga atgcagtgct gtaacgtccg ataagtgaag
    21121 cctgaatgtc ggagactttc ggtacagccg atccgtagat cacattcatc gcgttggtat
    21181 aaccagcgat ttgccccaca tcaggttgag taacgatagc tgtggaagtc cacttttctt
    21241 tggcaacggc cagataccct atagccaggg caatagcaac aatgacagag atgataattg
    21301 tcatcttgcc acgccacaac tgcaccagta aatcaatcaa atcaatctgt tccgggtcat
    21361 ggttttgccc agaaacatta ttattttcta ctctcatcat taccctaacg gacgaaataa
    21421 ttttaagtcg ccatagttta gcgataatcg ccatgaatgc tataggataa atgataaaaa
    21481 taatgaatta ttacaaaggg agcataagac aatcagggcg taaatagccc tgataacaag
    21541 atgttagtcg ctgccaaaga gatcgcgggt gtatacctta tctgccacat ctttaagctc
    21601 ttctgccatt cggttagaga taatgacgtc ggcttgttgt ttgaagatgg cgagatcacg
    21661 ttccaggcga gagttgaaga atgagtcttc tttcatcact ggctcgtaga tgatcacttc
    21721 aacgcctttc gccttgatac gtttcataat cccctgaata gaagacgcac ggaagttatc
    21781 tgaaccgctc ttcataatca gacgataaat acccaccact tgcggtttgc gtgacaaaat
    21841 ggcatcggca ataaaatcct tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt
    21901 attcggcaca gactgatagt tcgccagtaa ctgcttggta tctttcggca aacaataacc
    21961 accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc
    22021 ttcgataatc tggcgagtat tcagacctaa actttctgca tagctatcca gttcattaaa
    22081 gtatgctacg cgcatcgcca gataggtatt agcaaacagt tttatcgctt ctgcttcagt
    22141 ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc
    22201 aaaacgttct gcgcgttctg aacgctcacc aatgacaata cgtgaaggat ggagattatc
    22261 gtaaagggct ttaccttcgc ggaggaattc aggggagaat ataatatttt cagtgcgata
    22321 tttcttatgc atcgctgcag taaaaccaac gggaaccgtt gatttgatca ccataaccgc
    22381 ataaggattt atctcaacta cgtctttaat tactgattct acactggatg tattgaaata
    22441 attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag cataccggta
    22501 ggcttcattt ttatctaatg tagcattaaa gtgtatttta tctgattgca aaaactgctg
    22561 aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa
    22621 aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac
    22681 atagccggta ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga
    22741 atcatgatgt ttttagcatc ctgataagag ctaaaagttt taacgctacg ttgtcagatg
    22801 gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgata tttattaagt
    22861 taatattaag cagtgaaatt tagtctataa gataattggc aaaaaaaagc ccggcgcaat
    22921 atacgccagg cctcaattta ttgttagtta aataagatta atccagccat tcagtatgga
    22981 acacaccttc tttatcaatg cgcttataag tatgtgcacc gaaatagtca cgctgtgcct
    23041 gtatcaggtt cgcaggcaga acagcggcac ggtagctgtc ataataggca accgcagcgg
    23101 cgaaggtggg aaccgggata ccgttctgta ctgcataagc aacaacatca cgcagcgcct
    23161 gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta gcgatctgcg
    23221 gattttcggc atatgcatcg gtgattttct gcaggaactg cgcacggatg atgcagccag
    23281 cacggaaaat cttcgcgatt tcgccgtagt tcagatccca gttgtactct tcagacgcag
    23341 cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac agcgcacgac
    23401 gaactttttc gatgaactca gccttgtcgc cagctggctg cgcttgcgga ccagagagaa
    23461 ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca aacacagact
    23521 cggtaatcag cgacagcggt tctccgagat ccagcgcgct ctggctggtc catttaccgg
    23581 tacctttgtt agccgcttca tccaggatca catcaaccag gtagttaccg tcttcatctt
    23641 ttttagtgaa gatgtctttg gtgatgtcga tcaggtagct gctcagttca ccgttattcc
    23701 actcggtaaa ggtctgtgcc agttcttcgt tagagagatt caggccgcct ttaagcagag
    23761 aataggcttc agcaatcagc tgcatatcac cgtattcaat accgttatga accatcttca
    23821 catagtgacc tgcaccatcg gcaccaatat aggtaacgca tggctcccca tcttcagcta
    23881 ctgcggcgat tttggtcagg attggtgcaa ccagttcata ggcttctttc tgcccaccag
    23941 gcatgatgga aggtcctttc agcgcacctt cttcaccacc ggaaacaccg gtaccgataa
    24001 agttaaaacc ttcggcagaa agctcacggt tacgacgaat ggtgtcctgg aagaaggtgt
    24061 taccaccatc aatgatgatg tcacctttat cgaggtatgg cttgagggaa tcaatagcag
    24121 catccgtgcc tgcgcctgct ttcaccatta acaggatgcg acgaggcgtt tccaaagatt
    24181 caacaaactc tttcaccgta tagtaaggaa ccagtttctt gcctggattt tcggcaataa
    24241 cttcttccgt cttctcacgg gagcggttga aaatagagac ggtataacca cggctttcga
    24301 tgttgagcgc taggttgcgc cccatcactg ccataccgac tacgccgatc tgttgctttg
    24361 acattgttta ctcctgtcag gggttactgc gaactttaac gcaggtctat atgaagctta
    24421 cggtaactca ggttagaaaa gtattatatt tttactgacg ctaagcaatt cagtttttct
    24481 cagaaaattt ataaccatca ggattgcttt gttgccaatt ccaggcatcg cgaaccatct
    24541 catctaatcc caaacaggct ttccatccaa gttcaaggta tgctttagac gggtcggacc
    24601 aacattcagc aatgtcgcct gctcggcgct caaccaacac ataaggtata ttaacagagg
    24661 ttatccgttg aaaagcagcc aaaagttcaa gaaccgaata accatgccct gtgcctaaat
    24721 tgtaaacctt ataattgcta ccttcatctc ggtgcttcaa ggcagctata tgaccgtttg
    24781 cgagatccat aacatgaata aaatctctaa cgccagtacc atctttagtt tcataatcat
    24841 taccatagat ggataattgc ttatatttac cacttgcaac tgaacatata taaggcatca
    24901 aattatttgg aataccgtta gggtcttctc caatttttcc ggaaggatgt gcgccaacag
    24961 gattaaaata acgcaaaatt gttgttctaa aaaaaggata tgccaaagaa acatcatgta
    25021 atatgttctc aaccataagt tttgaagtcc catagggatt agtagtgcca cctacaggga
    25081 atgtttcttt tacgggtatc ttctctggag taccatatac agtggctgag gagctgaata
    25141 ttagattctt gatattatta ctaatcattt cctcaataag aacaagtgta ccgtatacat
    25201 tattatcgta gtactctgtt gggaattgaa ttgaatcgct tactgatttc aggccagcaa
    25261 aatgaataac atctgttatg ctgttttcag taaagatgga ttttaaaata acacgatctc
    25321 gaatatcacc attaatgata cagatatcac taccagtaat ttctttaacg cgatgcaaag
    25381 attcaactga agaattatga aaattatcaa gcactattac ttcatattgt tctttcagca
    25441 gcattaatac agtatgtgag ccaatatatc cagcaccacc cgtcactaat atagccatac
    25501 aacagcaccc tcatttaatc aaagcaacaa gactagcaga tagaatgtta ttaaagcttg
    25561 gataatttaa taactcttat gcagttataa tattaactaa agaatttgca gattcacttt
    25621 cattaaattt tgacaatgcg aatcttcgac tatttaatcc catagtgaca ataagctcac
    25681 gttcatgaat aaatgtttcc ataactaaag caagtttttg tgtatcccat ggaggtacaa
    25741 gatatccatt atatccatca attactgttt ctcgacaacc tggcacatcg gtcgtaataa
    25801 tcgcacgacc tattgccatt gcttcctgag tacttttggg gacaccttct cgataagaag
    25861 gtaatacaaa gacgctgata tcactcaaat attcttttat ataagagcac ggtcctaaaa
    25921 attcaacata accttccgct ttccaattta caatttgtgc agcatcaatt ccacccggat
    25981 tatcatcaaa atcaccagct atactaaatc ggaccattgg atattttttc tttattattt
    26041 tagccgcttc aacaaattca catacgccct tctctctcaa taatcttgcc accataccaa
    26101 aatggatctc cccacaaatt ggtggatgat aactaaattc attgagattt acacctatgc
    26161 caccaataac tttataatct ttgattttaa tattgttttt aacaaccaag tcatgatagt
    26221 catcattatt gagaaaaaga actttggttg caaaaggtaa tgtcatctta tataaaaaac
    26281 cttgaatctt tcttaagact atttttttaa acgagtcttt atctttattt tgtgtgaaac
    26341 aaaaacccaa cccttccaat aaagcgtaac gtttttttac gccagcaatc atagcggcaa
    26401 gagtaccata tataactggt tttgggaaaa agcaaagaac agaatcagga ttaatctctt
    26461 ttattttcct tgccagaaaa aaagtattaa aaaaatcaga
//
LOCUS       DAZGCS010000069        26073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGCS010000069 DAZGCS010000000
VERSION     DAZGCS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.069"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHK01_003026"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHK01_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO8097945.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHK01_003027"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHK01_003027"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO8097946.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHK01_003028"
     CDS             complement(941..1408)
                     /locus_tag="ACHK01_003028"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO8097947.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK01_003029"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK01_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO8097948.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK01_003030"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK01_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO8097949.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK01_003031"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK01_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO8097950.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK01_003032"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK01_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO8097951.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK01_003033"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK01_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO8097952.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK01_003034"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK01_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO8097953.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK01_003035"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK01_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO8097954.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK01_003036"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK01_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO8097955.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK01_003037"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK01_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO8097956.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK01_003038"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK01_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO8097957.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK01_003039"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK01_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO8097958.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK01_003040"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK01_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO8097959.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK01_003041"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK01_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO8097960.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK01_003042"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK01_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO8097961.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK01_003043"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK01_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO8097962.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK01_003044"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK01_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO8097963.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK01_003045"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK01_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO8097964.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK01_003046"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK01_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO8097965.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK01_003047"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK01_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO8097966.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK01_003048"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK01_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO8097967.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK01_003049"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK01_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO8097968.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHK01_003050"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHK01_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO8097969.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK01_003051"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK01_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO8097970.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK01_003052"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK01_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO8097971.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK01_003053"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK01_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO8097972.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK01_003054"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK01_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO8097973.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK01_003055"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK01_003055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO8097974.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK01_003056"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK01_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO8097975.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHK01_003057"
     CDS             15548..15916
                     /locus_tag="ACHK01_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO8097976.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK01_003058"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK01_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO8097977.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK01_003059"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK01_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO8097978.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHK01_003060"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHK01_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO8097979.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHK01_003061"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHK01_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO8097980.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHK01_003062"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHK01_003062"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO8097981.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHK01_003063"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHK01_003063"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO8097982.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHK01_003064"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHK01_003064"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO8097983.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHK01_003065"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHK01_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO8097984.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHK01_003066"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHK01_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO8097985.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHK01_003067"
     CDS             23095..23637
                     /locus_tag="ACHK01_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO8097986.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHK01_003068"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHK01_003068"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO8097987.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHK01_003069"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHK01_003069"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO8097988.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHK01_003070"
     CDS             25034..25291
                     /locus_tag="ACHK01_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO8097989.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHK01_003071"
     CDS             complement(25267..25821)
                     /locus_tag="ACHK01_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO8097990.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6705 a         6182 c         6754 g         6432 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgctaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tttacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcga aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag aaaaaatcct gaa
//
LOCUS       DAZGCS010000070        25098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGCS010000070 DAZGCS010000000
VERSION     DAZGCS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.070"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..2135
                     /gene="yihQ"
                     /locus_tag="ACHK01_003072"
     CDS             99..2135
                     /gene="yihQ"
                     /locus_tag="ACHK01_003072"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO8097991.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILIAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2181..3566
                     /gene="yihP"
                     /locus_tag="ACHK01_003073"
     CDS             2181..3566
                     /gene="yihP"
                     /locus_tag="ACHK01_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097992.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3609..5012
                     /gene="yihO"
                     /locus_tag="ACHK01_003074"
     CDS             3609..5012
                     /gene="yihO"
                     /locus_tag="ACHK01_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097993.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5080..5772
                     /gene="ompL"
                     /locus_tag="ACHK01_003075"
     CDS             5080..5772
                     /gene="ompL"
                     /locus_tag="ACHK01_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO8097994.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5863..7128)
                     /gene="yihN"
                     /locus_tag="ACHK01_003076"
     CDS             complement(5863..7128)
                     /gene="yihN"
                     /locus_tag="ACHK01_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8097995.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7230..8210)
                     /gene="yihM"
                     /locus_tag="ACHK01_003077"
     CDS             complement(7230..8210)
                     /gene="yihM"
                     /locus_tag="ACHK01_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO8097996.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8218..8928)
                     /gene="yihL"
                     /locus_tag="ACHK01_003078"
     CDS             complement(8218..8928)
                     /gene="yihL"
                     /locus_tag="ACHK01_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8097997.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9145..10968)
                     /gene="typA"
                     /locus_tag="ACHK01_003079"
     CDS             complement(9145..10968)
                     /gene="typA"
                     /locus_tag="ACHK01_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO8097998.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11341..12750
                     /gene="glnA"
                     /locus_tag="ACHK01_003080"
     CDS             11341..12750
                     /gene="glnA"
                     /locus_tag="ACHK01_003080"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO8097999.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            12924..13973
                     /gene="glnL"
                     /locus_tag="ACHK01_003081"
     CDS             12924..13973
                     /gene="glnL"
                     /locus_tag="ACHK01_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO8098000.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            13985..15394
                     /gene="glnG"
                     /locus_tag="ACHK01_003082"
     CDS             13985..15394
                     /gene="glnG"
                     /locus_tag="ACHK01_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO8098001.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15506..15616
                     /gene="yshB"
                     /locus_tag="ACHK01_003083"
     CDS             15506..15616
                     /gene="yshB"
                     /locus_tag="ACHK01_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO8098002.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15806..17179)
                     /gene="hemN"
                     /locus_tag="ACHK01_003084"
     CDS             complement(15806..17179)
                     /gene="hemN"
                     /locus_tag="ACHK01_003084"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO8098003.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17368..17877)
                     /gene="yihI"
                     /locus_tag="ACHK01_003085"
     CDS             complement(17368..17877)
                     /gene="yihI"
                     /locus_tag="ACHK01_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO8098004.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(17996..18162)
                     /locus_tag="ACHK01_003086"
                     /pseudo
     CDS             complement(17996..18162)
                     /locus_tag="ACHK01_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18459..19091
                     /gene="yihA"
                     /locus_tag="ACHK01_003087"
     CDS             18459..19091
                     /gene="yihA"
                     /locus_tag="ACHK01_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO8098005.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19473..22259)
                     /gene="polA"
                     /locus_tag="ACHK01_003088"
     CDS             complement(19473..22259)
                     /gene="polA"
                     /locus_tag="ACHK01_003088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO8098006.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22623..23555
                     /gene="yihG"
                     /locus_tag="ACHK01_003089"
     CDS             22623..23555
                     /gene="yihG"
                     /locus_tag="ACHK01_003089"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO8098007.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(23596..25026)
                     /gene="yihF"
                     /locus_tag="ACHK01_003090"
     CDS             complement(23596..25026)
                     /gene="yihF"
                     /locus_tag="ACHK01_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8098008.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLEVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         6112 a         6302 c         6048 g         6636 t
ORIGIN      
        1 gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt aataacagga
       61 aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca cgtccacagt
      121 tattagattt tcaatttcat cagaataacg acagttttac cctacatttt caacaacgtc
      181 ttattttaac ccatagcaaa gataatcctt gtttatggat tggctcaggt atagcggata
      241 tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa attgcgctta
      301 ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc cgtggttctg
      361 acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg ctggaactac
      421 aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa ccagaggacc
      481 atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgtggcaaa ccgttcccgc
      541 tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc tggcaggccg
      601 actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag cctacgtttg
      661 tcagcacgca gaagtattac tgccatgttg ataacagttg ctatatgaac ttcgacttta
      721 gtgccccgga ataccatgaa ctggcgctgt gggaagacaa agcaacgctg cgttttgaat
      781 gtgctgacac atacatttcc ctgctggaaa aattaaccgc cctgctggga cgccagccag
      841 aactgcccga ctggatttat gacggagtaa cgctcggcat tcagggcggg acggaagtgt
      901 gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggtcaacggc atctgggcgc
      961 aggactggtc cggtattcgt atgacctctt ttggcaaacg cgtgatgtgg aactggaagt
     1021 ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggaat caggagggcg
     1081 tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc tgcgaagaag
     1141 cggcacaaca cggctatctg gcaaaagatg cctctggcgg tgactatctg gtggagtttg
     1201 gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc tggttcaagg
     1261 aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct gacttcggcg
     1321 agtatctgcc caccgacact tacctgcata acggcatcag cgccgaaatt atgcataacg
     1381 cctggcccgc gctgtgggcg aaatgtaact acgaagccct tgaagaaacg ggcaagctcg
     1441 gcgagatcct tttctttatg cgcgccggtt ctaccggtag ccagaaatac tccaccatga
     1501 tgtgggcggg cgaccagaac gtcgactgga gtctcgacga tggcctggcg tcggttgtcc
     1561 cggcggcgct gtcgctggca atgaccggac atggcctgca ccacagcgac attggcggtt
     1621 acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg tgcgatttca
     1681 gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac aactggcagt
     1741 ttgacggcga cgcagaaact atcgcccatt ttgcccgtat gacctccgtc ttcaccaccc
     1801 tgaaacctta cctgaaagag gctgtcgcgc tgaatgcgaa gtccggcctg ccggttatgc
     1861 gcccgctgtt cctgcattac gaagacgatg cgcacactta caccctgaaa tatcagtacc
     1921 tgttaggtcg cgacattctg atcgctccgg tgcatgaaga aggccgtagc gactggacgc
     1981 tctatctgcc ggaggataac tgggtccacg cctggacggg tgaagcgttc cggggcgggg
     2041 aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat
     2101 gggcggcact gttcgcgtcg ttaaaaagca tctaatcaca tccgcccgtg gggaaactca
     2161 tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca actttacgcc
     2221 tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac atcctgctgg
     2281 atatcggtac actttatctt ttgaagtttt ataccgacgt tctggggctg ccaggcacct
     2341 atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat atgggtaccg
     2401 gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt ccttttattc
     2461 tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc acaccgtttg
     2521 atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga ctgtttttca
     2581 gcatgatgaa ctgctcctac ggcgcaatgg ttcccgctat caccaaaaac cccaacgaac
     2641 gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg
     2701 tgggattcgt gccagtgatg aatcttatcg agggtaatca gcaacttggc tatatcttcg
     2761 ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga
     2821 aagagcgtta tgtcgaaacc caaccaacca atccggcgca aaagcctggc ctgttgcaat
     2881 ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgtattgcc aacctctgca
     2941 ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta ttacacccag tacgtactta
     3001 acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt ttcatcggcg
     3061 tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc
     3121 tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggc tcggtcagct
     3181 ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgtt tgttaacagc ctgaactggg
     3241 cgctggtttc cgacaccgtc gagtacggcg agtggcgcac cggcgtgcgt tcggaaggca
     3301 cggtctacac cggcttcacc ttctttcgca aagtgtctca ggcgctggct ggtttcttcc
     3361 ccggctggat gctgacgcaa attggctatg tgccgaacgt cgcacaggct gaccacacca
     3421 ttgaagggtt acgccagttg atcttcatct acccaagcgc actggcggta gtcaccattg
     3481 tggcgatggg ttgcttctac agcctgaacg agaagatgta tgtccgcatt gtggaagaga
     3541 tagaagcccg taaacgcacg gcgtaataat aataaacgac gccctgcggg gcgttataag
     3601 gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg gaaaaaatcg
     3661 cctatggtat gggcgacgtc ggttcgaatt taatgctctg catcggtact ctgtatctcc
     3721 tcaaatttta taccgatgaa ttagggatgc ctgcttacta tggcgggatt atctttctgg
     3781 tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc gactcgcgga
     3841 aaaatattgg gcctaaaggc aaatttcgtc cctttatttt atatgcagca gttccggcgg
     3901 cgttaattgc aacgctccag tttatcgcca ccaccttttg tttgccggtt aaaacgacga
     3961 ttgccaccgc actgtttatg atgtttggac tttcatacag tctgatgaac tgctcgtatg
     4021 gtgcgatgat cccggcaatt accaaaaacc cgaatgagcg cgcgcagctc gcggcttacc
     4081 gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt ccgttgcagt
     4141 cgcttttttc tgactcaacc gtcggttatg cctgtgcggc acttatgttc tccattggcg
     4201 gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg gacacagcgc
     4261 caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgt aatccaccat
     4321 tgctggtttt atgcattgct aacctgtgta ctctggcggc atttaatatc aaactggcga
     4381 ttcaggtcta ttacacccag tatgtgctga atgatattaa tttattgtcg tggatgggat
     4441 tcttcagtat gggatgcatc ctcgtcggcg tattactggt gccagtaact gtaaaatgtt
     4501 ttggtaaaaa acaggtttat ttagctggca tggtgctgtg ggcggtgggt gatatactga
     4561 attatttctg gggaagtaac tctttcactt tcgtcatgtt ctcttgtgtc gccttttttg
     4621 gcacggcctt tgtaaacagc ctgaactggg cactggtacc agataccgta gattacggtg
     4681 aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc ttctttcgta
     4741 aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa attggttatg
     4801 ttcccaacat cgcgcaaagc gatgcgacat tacagggttt gcgtcagctc atttttatct
     4861 ggccttgtgc actggcaatt attgctgcat tgacgatggg attcttttac acactcaatg
     4921 aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg caaaaataaa gagatggcga
     4981 cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt tccattctgg
     5041 tgggtggcgg cctccctacg tttaaaaaat ggacttatta tgaaaaagat taatgcaata
     5101 attttattat cttctctgac ctctgcctcg gtatttgctg gcgcatacgt cgaaaacagg
     5161 gaggcatata atcttgcctc cgatcagggc gaggttatgc tacgtgtggg ttataacttc
     5221 gatatgggcg cgggtattat gttaaccaat acctacacct ttcagcgaga agatgaacta
     5281 aaacatggct ataatgaaat tgaaggttgg tatcctttat tcaaaccaac cgataaatta
     5341 accatccagc ccggtggctt aattaatgat aagagtatcg gttcgggtgg tgcagtgtat
     5401 ctggacgtca actataaatt tgtaccatgg tttaatctga cagtacgaaa tcgctataac
     5461 cataataact atagttcaac agatttgagc ggggaactgg ataataatga cacctatgaa
     5521 attggcacct actggaattt taaaatcacc gataaatttt cctatacatt tgagccacat
     5581 tatttcatac gcgtaaatga ctttaatagc agcaatggaa aagatcatca ctgggaaatc
     5641 actaatacct tccgttatcg cattaatgaa cactggctgc cttatttcga attacgttgg
     5701 ttagatcgta atgtcgaacc gtaccaccgc gagcaaaacc agattcgtat cgggacgaag
     5761 tatttcttct gatgtgaaac tgccggatgc ggcgtaaacg ccttatccgg cctacggttg
     5821 atacggtttt cgttggccgg attaagcaca tccggccatt aatcaagctt cagccgccag
     5881 catctctttc ttacgttggc taattcgctt aaccagcatt acggaaacca ccgcaccaca
     5941 gaatgcgaag caggccataa tgccaaacac gattttgtag ccaataatcc ccggatttaa
     6001 atccagaatg tagccataca gactgaagca gaacatcgcc ggagcgtaac caataaagct
     6061 acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc cgataggtgc
     6121 aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac atgccatccc
     6181 taaatagacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg cagcggtact
     6241 gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat ctgaaatcat
     6301 gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc cgtaagcccc
     6361 caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga atgtcaggcc
     6421 acagtaaacc gcgtaaacga agaagacgtt aaaagcaata agccagatag ttttgtcttt
     6481 cagcaccgag gtcatactgg tgtttttcga tgctccgtct tcttttttca cctcgacgga
     6541 cggtgcctct tctttgtcat tcagaacaaa gaaaatgata atgcctacgg caatcacaat
     6601 aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa accaggtaaa
     6661 tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg tttcgaagaa
     6721 gccaaacaac cgaccttgtt gttcgctatt gcccaatcga ctcaccgact tgagcaagac
     6781 cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga ggatgcccca
     6841 ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga gtgagaatga
     6901 catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa aacccactgt
     6961 cgtgacaaat gagtttaccg acatagcatt accaatttga ccattggtca aatggaaata
     7021 ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa ttgtaccgcc
     7081 acacaatgtt aatagactaa ataacgccca tttctttttc gtgagcataa catcccctaa
     7141 gcgattttta aaatcaatgt tttaaaggca ataagatacc gaccattcat atggaatggt
     7201 tttattaact gcgtatgagt taaaacttat tagtggttca gaagatggtt tgcctcttgt
     7261 ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc ttctttttct
     7321 ttacgtaacc gcgcatccag taaatgattt tctggcaatg gtgtttcact catctggcga
     7381 taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc atagtaatca
     7441 acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag gcatataagg
     7501 tgcgttaata acgctttgaa cggcatatca ccctgacctg aaatacaggc tttatgaccc
     7561 aggccacccg gttctttaac gatcaaggca tctttgatat ggacctgggt aatatgcggc
     7621 gccatcgttt ttaaagcgtc gatgggatgc tcatttgcat tgatcatgtt cgcaaaatca
     7681 aacagtaagg aaagagattc catctcactt tctttgacca gcgacaccag ctcatggctt
     7741 tttaaatctt catgctgttc gatcgtaaaa gtcaggccgc tgtcctgata cgtttcccgt
     7801 acataggcaa tgtcgttagc gatgatcgat aatacgtcgc gcaaattacc ttcataacgt
     7861 ggataaaaac gtacggacga tgccccagtt ttcaacgcaa tggcgacggc ttcgtcaata
     7921 gatgccttat ctgaagcgct ggtttcaata tgaatatcaa ggttcagacg gcgggcttta
     7981 tccccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg ctcgccatcc
     8041 agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag taaatcagca
     8101 ggcaacactc tttccagccg catatttaaa tgaaaggcat aagcatgtaa ataaagagga
     8161 agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcaccat aaaacaccta
     8221 cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta tctggacaat
     8281 attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac ccgcagaacc
     8341 gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca atttttttac
     8401 taccgtctct ttttggtagc acttttccca gactgcagaa aatgagtggt tttcaagttg
     8461 ttcaataaaa cccggagcaa cttcaggatt aatatatgtt tcgtgataga aaactttatg
     8521 tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggtg tcactgcaat
     8581 cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt ttctcggtaa acccccagga
     8641 aggttcccgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct ccgggctgta
     8701 attaaatcgc ggctgagtca cgaaccaccc tttacgatcc ttacggaaga ttttggattc
     8761 tgcctcgagg ttcagcaacg cctggcgcag cgtcatacgt ttaatgccca gcagctctcc
     8821 cagctctctt tctgaaggga gttttccacc cggcgtagtg atgccatctt ttaaccaccg
     8881 atccagtttc tcttttgcat tttctacggt ggattgattt cccgtcattg tttaatcctt
     8941 ttggtttaaa ccagatgagg catcttacgc ttacgccaaa aaagaaagca attaacggcg
     9001 tatgactttg cagataattg tgacaaagct ctctattgac gtaaccacag acttataagg
     9061 gagatactcg cattgctgag tgctcacgac aggcaaaaaa aaacctgcca tcgctggcag
     9121 gttttttatg actaaaaaac gaaattaatc gtctttcggt gcgcggttgg cgcgacgacg
     9181 atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggggtca cttctaccag
     9241 ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag gcggaaccag
     9301 aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac cggtcaggca
     9361 gtttacagtc aggtcgttag agcggctatg aataccgata atctgacctt cgtaaacttc
     9421 tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg cgaacgcgac
     9481 cgctttaccc tgaccgttag agatcagtac gccgttctga cgctgaccca cttcacccgg
     9541 acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg aagtcatggt
     9601 catgaactca gaacggaagc caatcagacc acggcttggg atcacgtagt cgagacgtac
     9661 gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc ccagcgcctg
     9721 cattacagaa ccctgatgct gttcttcaac gtccagcgtc acgttttcat acggctcttg
     9781 tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca gttcgaaacc
     9841 ttcacgacgc atgttttcga tcagaacaga caggtgcagt tcgccacgac cagaaacgcg
     9901 gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca acgttatgta ccagttcttt
     9961 gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac cgcagaacgg
    10021 cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga gtgccggcag
    10081 cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc caaggcccgt
    10141 gatcgccacg atatcgccag cttccgccag atcggtttcg atacgttcca gaccgaggtg
    10201 gcccagcact ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga tgatagtgac
    10261 ctgttggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa cgccaacata
    10321 gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt caacgtccgg
    10381 cgcaggaacg tgatcaacaa tcgcctgata cagcggggtc atgtcttccg ccatatcttc
    10441 gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga agtccagctg
    10501 ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa cccaatcagg
    10561 acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc cgtaagcaaa
    10621 cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt caaccaccag
    10681 cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt cggcgtgccc
    10741 cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt ttttcgcgag
    10801 gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct cttgggtttc
    10861 ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg tggttttacc
    10921 atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa ctttgcctca
    10981 ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac aggatcacaa
    11041 acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg gagcataaaa
    11101 agggttatcc aaaggtcatt gcaccaacat ggtgcttaat gtttccattg aagcactata
    11161 ttggtgcaac attcacatcg tggtgcagcc cttttgcacg atggtgcgca tgataacgcc
    11221 ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttac ggcgacacgg
    11281 ccaaaataat tgcagatttc gttaccacga cgaccatgac caatccagga gagttaaagt
    11341 atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt tgttgatttg
    11401 cgcttcaccg atactaaagg taaagaacag cacgtcacta tccctgctca tcaggtgaat
    11461 gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg ctggaaaggc
    11521 attaacgagt ccgacatggt gctgatgcca gacgcatcca ccgcagtgat tgacccgttc
    11581 ttcgccgact ccaccctgat tatccgttgc gacatccttg aacctggcac cctgcaaggc
    11641 tatgaccgtg acccgcgctc cattgcgaag cgcgccgaag attacctgcg ttccactggc
    11701 attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga tgacatccgt
    11761 ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg cgcatggaac
    11821 tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa aggcggttac
    11881 ttcccggttc caccggtaga ctcggctcag gatattcgtt ctgaaatgtg tctggtgatg
    11941 gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc tggtcagaac
    12001 gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca gatctacaaa
    12061 tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat gccaaaaccg
    12121 atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa aaacggcgtt
    12181 aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta ctacattggc
    12241 ggcgtaatca aacacgctaa agcgattaac gccctggcaa acccgaccac caactcttat
    12301 aagcgtctgg tcccgggcta tgaagcaccg gtaatgctgg cttactctgc gcgtaaccgt
    12361 tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat cgaagtacgt
    12421 ttcccggatc cggcagctaa cccgtacctg tgctttgctg ccctgctgat ggccggtctt
    12481 gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct gtatgacctg
    12541 ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga agcactgaac
    12601 gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcactga cgaagcaatt
    12661 gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac tccgcatccg
    12721 gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc ggcgcaacaa
    12781 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
    12841 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
    12901 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
    12961 tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc cattacgcca
    13021 accctgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acaccgttac
    13081 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
    13141 ggcaaggttt taccgataac gaagtgacgc tggtcatcga cgggcgctcg catatccttt
    13201 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggct ccgatggata
    13261 accagcgccg cttaagtcag gaacagctac agcacgccca gcaggttgct gcccgtgatt
    13321 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
    13381 agctactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
    13441 aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcag ctgcccggta
    13501 cgcgcgttac cgaaagtatt cacaaagtag ctgaacgcgt ggtaacgctg gtgtcgatgg
    13561 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
    13621 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
    13681 tggggccgga gggtggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
    13741 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
    13801 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
    13861 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
    13921 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
    13981 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
    14041 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
    14101 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
    14161 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
    14221 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
    14281 tgattatctg cccaaaccgt ttgatatcga cgaagccgtc gcgctggttg agcgcgccat
    14341 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
    14401 tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
    14461 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
    14521 tgccctgcat cgccacagtc cgcgcgccaa agcgccgttt atcgcgctga atatggcagc
    14581 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
    14641 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggtacat tattcctcga
    14701 cgaaattggt gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
    14761 tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
    14821 cactcaccag aatctcgaac agcgagtgca ggaaggtaag ttccgtgagg atctgttcca
    14881 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
    14941 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
    15001 actgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
    15061 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
    15121 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
    15181 ggacagctgg gcgacgcttc ttgcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
    15241 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
    15301 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacaccct
    15361 gacgcgtaag ttaaaagagc tggggatgga gtgattcaca gcttgtgtgt aaagattgat
    15421 tattgagcgc aaattgctgg tattttacgc tttactgttc cgataagttc agtatgatct
    15481 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
    15541 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcaatgat
    15601 tgggctgttt agttgagttt cagattgctg atgaagtgct ctttgttcat gccggatgcg
    15661 gcgtgaacgc cttatccggc ctacaaaagc aatattttca acaggttttg taggcctgat
    15721 aagcgtagcg catcaggcaa tcttgttctc agcacattga tggcgctttc gttttctact
    15781 ttgtaaacga agcgccattc actatttaaa tcacccgtga gaactgctgc atccgcgctt
    15841 tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag cgacctttcg
    15901 ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct aacggggcga
    15961 gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt ttctcaatag
    16021 gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg cgaatacagt
    16081 catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatcc acttgctgat
    16141 agtactgctt caactctttc tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg
    16201 aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctgg aagttacgat
    16261 gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc cggacgggca aagtgatcca
    16321 taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc aggatatcga
    16381 gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg
    16441 tcggcagatg cgcgtagtta aagacactca gacgatcggg gttcagctcc gccacacgtt
    16501 tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt
    16561 tggtggaggt aaaaccaatc tcacgcgcat ggttaagcag tgcaaagatg aactcttcat
    16621 cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg cccatgctca
    16681 ggcgattaaa gccttcggcg cgtaaatgat cgagtacatc cagttcgatt tcccgcggat
    16741 cgacttcgat cgaaatctcc gcatcggcat tgaactggaa gttttcgcgc agcagcttca
    16801 tcaggcggct gatttgcgct ttattcagat acgtcggcgt tccgccgccc cagtgcaatt
    16861 ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc tccagcgcgt
    16921 ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa ccgcagaagt
    16981 aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc tcaggatagc
    17041 gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg
    17101 ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc agatcccagt
    17161 cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc tacggcgtac
    17221 cggcggatgc ccgtttcggg tcattagccg gttgcgcagc cacttttgtc gccgcgacaa
    17281 ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg ttatcagcag
    17341 aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca tcatgtcttc
    17401 ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga gtttctgcat
    17461 caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg cactcagggt
    17521 ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt ccagtaattc
    17581 caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct gtttggtgac
    17641 tttttcagtc acgcccaatg gaataggggt tttactgcca atacgtggat cttttggtgc
    17701 gttctggcct ttgctgcctg acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg
    17761 accacgacgt tttttctgac gcttgcgatc acgagcttcc tgatccagct cctcgcgtgt
    17821 ttttcgacgc gcttttgcat gacctttgct gcgtgagttt gaagatgatg gtttcataat
    17881 ggttcgtctt ttagccgttt tattcagtat agatttgcgg cggaatctaa cagaaagcaa
    17941 gcaaagaaaa aaggcgacag attactctgt cgcctttttt cctgactcat aacccttaac
    18001 gggtcttaca atccttgcag gccgccaaca caccctgttt ttcctttagc cggaacaatc
    18061 cgtgttgatt ccatttccgt ttaattacgt ctccggacgt ttgtcttcct gacaatcctc
    18121 tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc attgttcctg ttagcgtcct
    18181 cgctctatgg gtgctacttt acgcctttgc tttaaacaaa caatcaaccg ccatcagtat
    18241 ttgcacattt tcagagtttt atataaaagc aattcactga tttatatagc ttatttgaca
    18301 tgcatcgcag aattttctct gctatttcgc ataaagacta tcggcaatgt ctcacaaagc
    18361 gtatggcttt tacttacaac gctcacgccg aaaaatccgc cttttattat ggttcaggta
    18421 taatcgccac aaagctttga ctgacggaga cgaccgcttt gactaatttg aattatcaac
    18481 agacgcattt tgtgatgagt gcgcctgata ttcgccacct accttccgat accggaattg
    18541 aagtggcttt tgcaggccgt tccaacgcag gtaaatccag cgcgctgaac acgctgacta
    18601 accagaaaag cctggctcgt acctcaaaaa ccccagggcg cacccagctt atcaacctgt
    18661 ttgaagtggc tgacggcaag cgtctggttg acttgcctgg gtacggttat gcggaagtcc
    18721 cggaagagat gaagcgcaaa tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga
    18781 gcctgcaagg tctggtggtg ctaatggata ttcgccatcc gctgaaagat ttggatcagc
    18841 agatgattga gtgggcggta gacagcaata tcgccgttct ggtgctgctg accaaagcgg
    18901 acaaactggc aagcggcgca cgtaaagcgc aattgaatat ggtgcgtgaa gctgtactgg
    18961 cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgtt gaagaaacaa ggcgtggaca
    19021 agctgcggca gaaactggat acctggttta gcgagatgca gcctgtagaa gaaacgcagg
    19081 acggcgaata attttcttgc cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa
    19141 ttcaatagat tacaattcta ccgttggcct gataaacata gcgcatcagg cattacggat
    19201 cttttctttc gcccaataaa aaacgcccca gtcattactg actggggcgg ctaaaatatt
    19261 cagccaaatc cgattacgtg aagtaaaagg tctgaaagat agaacatctt acctctgtac
    19321 cctacgcgac taactttact cttttttgtt cagttcagaa agcacttttt gtaatttttt
    19381 ttcagttgtt gcataggaat ttctaatagc catcacaaaa cgtgacagct tatgttgctt
    19441 acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca
    19501 ctccccactt ccaccagcaa cggcacatcc agacgggtac agttttccat cagttgatga
    19561 atctgcttcg cgacggcatc aacatcatct ttatgaactt caaataccag ttcatcgtgt
    19621 acctgcatga tcatacgtac acgcggttgc tcagcctgta accacgcatc aacggcaatc
    19681 atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt
    19741 tcagccgctg cacgacgagc accattgctg gatttgatat ccggcagata cagacggcgt
    19801 ccgtccagcg tttcaacgta gccctgctct ttcgcctgag cacgggtgcg ttccatatac
    19861 tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt
    19921 ggaatgttca attgccgcgc cagaccgaaa gcactcatgc cataaatcag accaaagttg
    19981 atcgctttcg cgctacggcg ttgctcgctg gtgacggttt ccagtggcaa accaaacact
    20041 tctgccgccg ttgcccggtg gatatctttt ccttccgcga atgcggtcag caagcctttg
    20101 tcacgcgaaa gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc
    20161 acataatcct ctggcgcaat aaacgcctgg cggatacgac gaccttcttc gttacgcacc
    20221 ggaatgtttt gcaggttagg atcggttgac gataaacgtc ccgttgcagt tactgcctgg
    20281 tgataagagg tatgcacacg cccggttttc gggttgatca tcagcggcag cttgtcggtg
    20341 taggtcgatt tcagcttcgc cagaccacga tactccagaa tcacttttgg caacggatag
    20401 tccagcgcca gttcttccag tacctcttcc gacgttgacg gcgcgccacc cggcgttttc
    20461 ttcagcggtt taataccctg tttttcaaag agaatggttt gcaactgctt ggtggaagaa
    20521 aggttaaatt cttcacctgc aatttcatgc gctttctttt ccagctcagc cagacgcaag
    20581 gtgagctctt cagaatgatt gtgcagcact ttcggatcga tcttcacacc gttacgttca
    20641 atgcgtgaaa gcaccggcac cagcggcatt tcgatattct cgaagacgtt caacggccct
    20701 ttgtgttttt gcagatctgg ccacattttc agatgcaact gcaaggtgac atcagcatct
    20761 tcggcggcgt aacgtccggc ctcttcgagg gcaatctggt taaaggtcag ttgatttttg
    20821 cctttacccg caatctcttc aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg
    20881 ctgtccatat cgtgacgccc ggcaacgcta ttgagaatgt aggactccag catggtatca
    20941 aacgcaatcc cacgcagctc aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg
    21001 ttttgcccga ccttcagcgc cttttcatct tccagcagcg gttttagcaa ctcgagtgca
    21061 cgctcgcgag agatttgatc gggcgcgtca agataatcat gagcaaccgg aatatatgcc
    21121 gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt tagcagagat gttatcaagg
    21181 ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct
    21241 ttcagtgttt cttcatcaag gatggtgacg tagttgtcat aagaaatcac cgttgccgtc
    21301 acttctggcg cttcgtctgc aacactggtt tcctgtggct tcgcggctgg ttttgcccct
    21361 ttggcctgta accatttgcc cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt
    21421 ttgaacagcc ccaacaactc ttccgctgcc ggttgctgca cttccagttg ttcacaggtc
    21481 agctccagtt caacgtcggt tttaatcgtc gccagctggt atgagagata agcaacttct
    21541 ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt
    21601 ttttctggct cggcatacag cgtatccagt ccgccaagac cttgcagcaa tgcctgcgcg
    21661 gttttttcac cgacgcccgg tacgccagga atgttatcag aggagtcacc catcagcgcc
    21721 aggaaatcga tgatcagttc tggcggcacg ccgtacttat tcaccacctc ttccggtccg
    21781 aggatggtat tcgtcatggt attgataagc gtaatatttg gcgtcaccag ctgcgccata
    21841 tctttatcgc cagtgctgat cagcaccgga cgcccggctt tttcggcttc gcgcgccaga
    21901 gtaccgataa cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag tcccatcgct
    21961 ttaaccatcg cgtgcaaggg ttcgatttgt gcacgcagat cgtccggcat tggcgggcga
    22021 tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt ttcccttggc gtcaaagacc
    22081 accgctgcat gcgtcggttt atattgcatg atcagactgc gcagcatgtt gaggacacca
    22141 tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg
    22201 cgataaagat aagatgaacc atctacaagg ataagtggat tttgggggat ctgaaccata
    22261 atgtccgtgc ctgtttatca gattatgggt aaaggatgcc acagacagga tgaaaacatg
    22321 agtttttcac cgcttttcgt gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt
    22381 catgtggata actttgtgca taaattattt acatcgcttt taatttatta tcatgctttt
    22441 tacagcatga tgtttgtctt tgtttttcat gcagttaaaa ataaatccat ccctaataat
    22501 tgagataatg ggattttttc taatgtggat agatatgaat tatttttctc cttaaggatc
    22561 atccgttatt tgggtcgttt tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt
    22621 ctatggctaa ccttttgaat aaattcatta tgacgagaat actcgctgcg ataacccttt
    22681 tgctgagtat cgtattgacc attttggtca ctattttttg ttccgtcccg atcatcattg
    22741 ccgggattgt aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt
    22801 gtgattttat gatgtattgc tggtgtgaag gtctggcggt attactgcat ctgaacccac
    22861 acctgcaatg ggaagttcac gggctggaag ggttaagtaa gaagaactgg tatctgctta
    22921 tttgtaacca ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca
    22981 ttccgatgaa taaatatttc ctcaagcaac agctggcctg ggtgccattt cttggcctgg
    23041 cgtgctggtc gctggatatg ccgtttatga agcgttattc ccgcgcttat ttattacgtc
    23101 atccggagcg acgtggtaaa gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc
    23161 tgcatcccac caccattgtc aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc
    23221 agcaaaccca ctccactttt caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg
    23281 cgctaaatgt actgggcaaa caattcgata aactgcttaa tgttacactg tgttatccgg
    23341 acaataaccg tcagccgttc ttcgatatgt taagcggtaa actgacgcgg attgtcgtac
    23401 atgtggattt acagcctatt gccgatgagt tacatggcga ctacatcaat gacaaaagct
    23461 ttaagcgcca ttttcagcaa tggctgaact cattgtggca ggaaaaagac cggcttctga
    23521 cgtctctcat gtcatcacag cgtcagaata agtaagtttt actcgcctct gaatgagcag
    23581 aggcgagtga gtattttaat gaacaaaacg tccggcacga gacataaatt cttcttcgct
    23641 catctcctgt ccgttaaaaa caactttacc cggtgtataa cgcagctgca gcgaggcggt
    23701 attgttgtca atcgtgatta actgaaacat ctgacccaat gtcatcatcc cgctgatttg
    23761 tttatcagcc tgcttttgcg ctttttccgc atccattcct tcagataaat taagctgttt
    23821 tgcggtttct gtgacgacat taagcggtaa cttcacatca aaattgagcg atttgatatc
    23881 tttgtttgtg gatgatgaag atttggctgg gtcggcaata ctgatatcca gattggcatt
    23941 tagttcgccg agtgcgttct tccatcttac cggttgttta atggtcggct cacttttttg
    24001 taataacggt aaatactctt tgaacaatgc agcattcact tcttgcagag caacttcatc
    24061 gtttgctaac tcagggtgta cagcaagctg cttctgcata gcaatgttat actggatgat
    24121 aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact caccactacc
    24181 gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga cattgatata
    24241 atctttgtct ttaacgcgat ccagcatggt ttttgcaacg aaagcatcga tttgggcaac
    24301 gtcttccccg tgattgataa tgttaatctg attaaatttc gactcgcttt caccaaccgg
    24361 aaatttcttt tcttctaatc gcgccagaga atcaatggta aaactttttg ccgttacttt
    24421 gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat ccaggttaga
    24481 caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa actttccata
    24541 ctctgcaggg gcaacctcta atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt
    24601 cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca gttcattatt
    24661 cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca ttaacattgt
    24721 gatgggcaac ggaccgtgct caacatccac atcaaataca actttctggc ctggcttgat
    24781 attgagatcg ggtgcaccgt tatcgaatgt catttgcatt tgaaaacggg aattaaaaaa
    24841 gcctttgtca aaattttgat aacttaacgt catatcgtag gcatgttcac ctttcttttt
    24901 agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgtg tacctgtgta
    24961 ccatgtgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag ctgacttacg
    25021 tatcatataa atattccctg ataaaacagc aaaactttga atatccactt atgctgaaat
    25081 aatgtccagc ggcaggat
//
LOCUS       DAZGCS010000071        24860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGCS010000071 DAZGCS010000000
VERSION     DAZGCS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.071"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHK01_003091"
     CDS             <1..759
                     /locus_tag="ACHK01_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8098009.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHK01_003092"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHK01_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO8098010.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHK01_003093"
     CDS             2581..3900
                     /locus_tag="ACHK01_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO8098011.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISTPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4277..5659
                     /gene="dosC"
                     /locus_tag="ACHK01_003094"
     CDS             4277..5659
                     /gene="dosC"
                     /locus_tag="ACHK01_003094"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO8098012.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5684..8083
                     /gene="dosP"
                     /locus_tag="ACHK01_003095"
                     /gene_synonym="pdeO"
     CDS             5684..8083
                     /gene="dosP"
                     /locus_tag="ACHK01_003095"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO8098013.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8341..8922
                     /gene="ddpX"
                     /locus_tag="ACHK01_003096"
     CDS             8341..8922
                     /gene="ddpX"
                     /locus_tag="ACHK01_003096"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO8098014.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8936..10486
                     /locus_tag="ACHK01_003097"
     CDS             8936..10486
                     /locus_tag="ACHK01_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8098015.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVESDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10488..11510
                     /locus_tag="ACHK01_003098"
     CDS             10488..11510
                     /locus_tag="ACHK01_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8098016.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGYLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11507..12403
                     /gene="ddpC"
                     /locus_tag="ACHK01_003099"
     CDS             11507..12403
                     /gene="ddpC"
                     /locus_tag="ACHK01_003099"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO8098017.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12400..13386
                     /locus_tag="ACHK01_003100"
     CDS             12400..13386
                     /locus_tag="ACHK01_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8098018.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQLLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13379..14305
                     /locus_tag="ACHK01_003101"
     CDS             13379..14305
                     /locus_tag="ACHK01_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO8098019.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14361..14792)
                     /gene="osmC"
                     /locus_tag="ACHK01_003102"
     CDS             complement(14361..14792)
                     /gene="osmC"
                     /locus_tag="ACHK01_003102"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO8098020.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15138..15353
                     /gene="bdm"
                     /locus_tag="ACHK01_003103"
     CDS             15138..15353
                     /gene="bdm"
                     /locus_tag="ACHK01_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO8098021.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15455..15592
                     /gene="sra"
                     /locus_tag="ACHK01_003104"
     CDS             15455..15592
                     /gene="sra"
                     /locus_tag="ACHK01_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO8098022.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15749..17446
                     /gene="maeA"
                     /locus_tag="ACHK01_003105"
     CDS             15749..17446
                     /gene="maeA"
                     /locus_tag="ACHK01_003105"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8098023.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRMEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17580..18590
                     /gene="adhP"
                     /locus_tag="ACHK01_003106"
     CDS             17580..18590
                     /gene="adhP"
                     /locus_tag="ACHK01_003106"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO8098024.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18737..19021
                     /locus_tag="ACHK01_003107"
     CDS             18737..19021
                     /locus_tag="ACHK01_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8098025.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19072..19725)
                     /gene="fdnI"
                     /locus_tag="ACHK01_003108"
     CDS             complement(19072..19725)
                     /gene="fdnI"
                     /locus_tag="ACHK01_003108"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO8098026.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIIEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIILRPYFSQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19718..20602)
                     /gene="fdnH"
                     /locus_tag="ACHK01_003109"
     CDS             complement(19718..20602)
                     /gene="fdnH"
                     /locus_tag="ACHK01_003109"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO8098027.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20615..23662)
                     /gene="fdnG"
                     /locus_tag="ACHK01_003110"
     CDS             complement(20615..23662)
                     /gene="fdnG"
                     /locus_tag="ACHK01_003110"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23075..23077),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8098028.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLLWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKLLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23894..24775
                     /gene="yddG"
                     /locus_tag="ACHK01_003111"
     CDS             23894..24775
                     /gene="yddG"
                     /locus_tag="ACHK01_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO8098029.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6063 a         6338 c         6300 g         6159 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggtgtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta catagtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaccccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gccgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcactgct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccatgc ttactttgta acaagccggt gctttaccca ccggcgtgtt acaccttgtg
     3961 aaatatccca ctagtaatca tgcttacata agtcaaatta accacgcaat taaccacact
     4021 tagttaaaca gctataaatc cctaataaaa atgtatgaat atgaaaaacc atacgatatt
     4081 cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
     4141 tgaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
     4201 gtgcagttag tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat ttcccagcct
     4261 tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact
     4321 tgtcgaacag gcagatcctc tcattcgtgc taaagccgca gaaattgccc ttgcgcatgc
     4381 tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt
     4441 cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa
     4501 cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc
     4561 ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct
     4621 gaaaaaaatc ctctatccgg taatcttctc ttcggattat tctgccgcag aaaaacttca
     4681 ggtctaccat ttctcgatta acagtattga tatcgctatg gaagtgatga cccgcgcgtt
     4741 tacctttagt gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttact
     4801 ggaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga
     4861 tattatctat aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc
     4921 tgattttggc ctgtggttta accataaagg tcgacattat tttagcggca ttgccgaagt
     4981 aggccatatc tcccgtctga ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa
     5041 caccaggaat ttgaataaca gaagtttgcg ggtgaagttt ctattacaga taagaaatac
     5101 cgtatcgcaa attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt
     5161 cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg
     5221 cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga
     5281 taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa
     5341 agtctctcag gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg
     5401 cgatgaattt atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga
     5461 acgtattcgc agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc
     5521 cctctcactt tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat
     5581 tcaaatagcc gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact
     5641 ctggaaagcc agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc
     5701 ggataatgcc gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt
     5761 gttaattaac gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg
     5821 gtacaaacgt gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg
     5881 tcctgcgcat cctgaataca ttcgtcataa ccgtgaaggc ggtaaagcgc gtgtcgaggg
     5941 gatgagtcgg gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc
     6001 gctatcgaaa gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag
     6061 cgtagaaatg gcgcaaaaag aacagactcg acaattgatt attgccgttg accatctcga
     6121 ccgaccggtg attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac
     6181 cgaaatgttt ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa
     6241 cattcctgaa ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc
     6301 ccgcgatcag gatgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc
     6361 ctctatcagc ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgaccttctc
     6421 ggatatcacc gaagaacggc aaatccgcca gcttgaaggc aatattctcg ccgccatgtg
     6481 cagcagcccg ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgttct
     6541 caacgaatcg catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc
     6601 atcttcgcac ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg
     6661 tgatggcgcg cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc
     6721 ctttatcgaa cgcgtggcag atatcagtca gcatatggcc gcgctggcgc tggaacagga
     6781 aaaaagccgc cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa
     6841 tcgcaataac ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccggtggt
     6901 gtatctcatc ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc
     6961 cgatcaggca ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta
     7021 tctctgtcgt atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa
     7081 cattacccaa atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga
     7141 taaacccttc ccgcttacct tgagtattgg catcagctac gacgtgggta aaaaccgcga
     7201 ttacctgctc tccactgctc acaatgcaat ggattatatt cgcaagaatg gcggtaacgg
     7261 ctggcagttc ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc
     7321 agcgctgaaa gaagcgatta gcaataacca actgaaactt gtttaccagc cgcaaatctt
     7381 cgcagaaacg ggtgaactgt acggcatcga agcccttgcg cgctggcacg atccccagca
     7441 tggtcatgtg cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa
     7501 tattgggcgc tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa
     7561 tattcatatc ccggcgcttt ccgttaactt gtcggcactg cactttcgca gtaatcaact
     7621 gcctaatcag gtgtctgatg caatgcacgc ctggggtatt gacggccacc agctgacggt
     7681 agaaatcacg gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat
     7741 cctgcgtgat atgggcgtgg gcttatcggt agatgatttt ggtacgggct tttccggatt
     7801 atcccgctta gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg
     7861 ttgtctgacc gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag
     7921 cctcaattta accgtcgtgg cggaaggggt cgaaaccaaa gagcaatttg agatgctacg
     7981 caagatccac tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgaaga
     8041 aattccaggc tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg
     8101 cactcgcggg ttttcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga
     8161 ttccgggaga ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa
     8221 atgttgatca aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca
     8281 tggcatgaga tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt
     8341 atgtcggata ccaccgaact ggttgattta gccgtaatct tccctgatct ggagatcgaa
     8401 ttgaaatacg cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt
     8461 ctgctacaca aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca
     8521 ggcttacaac tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg
     8581 caagcctgcc cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt
     8641 ggcacggcga tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca
     8701 gggttcgatg aaatgcacga gcgctcccat gcctatcacc cttccgtccc gcccgccgct
     8761 cagcgcaatc ggctgttgct gaatgcgata atgactggtg gcggttttgt cggtatctcc
     8821 agcgaatggt ggcacttcga attaccccag gcagcgagtt accctctgct cgctgatcaa
     8881 ttcacctgtt ttatatcccc tggcacacaa cacgtcagtt aatcaggaaa ctgtcatgaa
     8941 gagatcgata ccgtttcgtc ccacattgct cgcgctcgtc cttgccacaa atttcccggt
     9001 tgcgcacgcc gccgtaccga aagatatgct ggtgattggt aaggccgccg atccacaaac
     9061 cctcgacccg gcggtaacaa tagataataa cgactggacg gtgacctacc cgtcttatca
     9121 gcggctggtt cagtacaaaa cggacggtga taaaggctca accgacgttg aaagcgatct
     9181 ggcaagtagc tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaggacaa
     9241 tgctaaattt gccgatggca caccggtcac tgccgaagca gtaaaactct cttttgagcg
     9301 gctactaaaa atcggccaag ggccagcaga agcatttccc aaagatttaa agattgatgc
     9361 tcccgacgaa catacggtga agtttaccct tagccagcca ttcgcaccgt tcctctacac
     9421 gctggcgaat gacggtgcat ccattatcaa tccggcggtc ttaaaggaac atgcagcgga
     9481 tgatgctcgc ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag
     9541 ctggcaaaaa ggtcagcaat tagttctggt gccaaatccg cattaccccg gcaataaacc
     9601 gaactttaag cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct
     9661 ctcccgtggc gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa
     9721 gcaggaaaat aaagtcaatg tggcagagta tccgtcactg cgcgttacct atctgtatct
     9781 gaataacagc aaagcgcctc ttaatcaggc ggatctgcgt cgggccattt cctggtctac
     9841 cgattatcag ggcatggtta acggcattct gagtggtaac ggaaaacaga tgcgcggccc
     9901 gattccggaa ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac
     9961 gaaagccaaa gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta
    10021 ctccgataac gatccgaact gggagcctat tgctttggcg acacaatcca gtctcaacaa
    10081 gctgggcatc aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg
    10141 taaaggtgat tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat
    10201 gtttatgaat tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta
    10261 tgaaaacagt gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca
    10321 gcgtacccgg gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta
    10381 cctgttccag aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa
    10441 tcccatgctg gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga
    10501 gtattttacg ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta
    10561 cgtttattat ctcgcatctg atccctggcg atccggcgcg gttactggcg ggtgaccgcg
    10621 ccagcgatgc tatcgtggaa aatattcgcc agcaactggg actggaccag ccactgtatg
    10681 tacagtttta ccgctacgtc agcgacctgt ttcatggtta cctgggaaca tccattcgta
    10741 ccgggcgtcc ggtgctggaa gagttgcgta tatttttccc ggcgacgctg gagctggctt
    10801 ttggtgccct gctgctggca ctcctgattg gcatcccgct gggcatactc tctgcagtct
    10861 ggcgaaatcg ctggctggat catctggtgc gaataatggc cattaccgga atctccacac
    10921 ctgcgttctg gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc
    10981 ccggcggcgg aaggcttgat gactggctgg atccaccaac gcacgttacc ggcttttatc
    11041 tgctcgatgc gctgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca
    11101 tcttaccggc attaacgctg gcgttcgttc acctgggaat tgtcgctcgc cagatccgct
    11161 cagcgatgct ggaacaattg agtgaagact acattcgtac cgcccgggcc agcggcttgc
    11221 ccggctggta tatcgtttta tgttatgcgc tacccaatgc gttgatccca tcgattaccg
    11281 tattgggttt ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg
    11341 cctggcccgg aatgggtgca tgggtagtaa catcaataca ggcgctcgac ttcccggcag
    11401 tgatgggctt tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt
    11461 tgctctattt gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga
    11521 ggaaacgtcc gccgtacgcc cacaaaaaca aacgcgattt aacggtgcaa aactggtatg
    11581 gatgctgaaa ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctatt
    11641 gatgatgatt ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc
    11701 ccgccttttg ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct
    11761 gttcagccgc gtactggtcg gcagtcagca atcaattctc gccggattag tcgtggttgc
    11821 cattgcgggt atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc
    11881 agacgccatc atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac
    11941 aatggcactg gctgccgctc tcgggccgag tttatttaac gccatgctgg cgattgctat
    12001 tgtgcgaatt cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta
    12061 tacctatgtt caggcggcga aaacctttgg tgcgtcgcgt tggcatctga tcagctggca
    12121 tattttacgt aactccctgc cgccgctgat cgtgcaggca tcgctggata tcggtagcgc
    12181 gattttaatg gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga
    12241 atggggcgcg atggtagcga atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc
    12301 atttccgggg gcagcgattt tgcttaccgc cgttgggttt aatctctttg gtgatggtat
    12361 tcgcgatctg cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac
    12421 attcaacagc tgcatttgag tttccccggt tttaacggtg atgttcacgc gctcaacaat
    12481 gtgtccttgc agattaaccg cggcgaaatt gtcggtctgg tgggagaatc cggctcaggt
    12541 aaatcagtca ccgcaatgct gattatgcgt ctgctacaga cgggcagtta ttgcgtacat
    12601 cgggggcaga tttcactgct gggagacgat gttcttaacg ctcgggaaaa acagcttcgt
    12661 cagtggcgcg gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg
    12721 acacgtcgaa taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt
    12781 cgggaagcca gagctaaagc gattgccctg ctggaagaga tgcaaatccc ggatgccgtg
    12841 gaagttatgt cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt
    12901 gcgctggcat tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg
    12961 gacgtcacgc tacagttgca gctactgcgt ttgcttaaac ataaagcccg cgccagtgga
    13021 actgcggtac tgttcatcag ccatgatatg gtcgtggtgt cgcaactgtg cgatagcgtt
    13081 tacgtgatgt atgccggaag cgtaattgaa agcggcgtga cggcagacat tatccatcat
    13141 ccccggcatc cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc
    13201 cagccattac ccgccattcc agggacggta ccaaacctca cccatttgcc tgaaggctgc
    13261 gcttttcgcg atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca
    13321 gcgtgtggtg acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt
    13381 gtctgacacg ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg
    13441 gcttggtaaa actacggaac atgttcatgc cattaatggt attgatttac agatccgccg
    13501 tggtgaaacc ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct
    13561 tttaatgggc atgctgcaac cgagccacgg gcagtacatc cgttcaggct cacaacgcat
    13621 tatgcagatg gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg
    13681 catcatcaca gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt
    13741 ggcagaggag ctggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca
    13801 tgcgttctcc ggcgggcagc ggcaacgtat cgccattgcc agagcactct cttcgcagcc
    13861 tgacgtgatt gtgcttgatg agccaacctc tgcgctggat atctccgtgc aggcgcagat
    13921 cctcaattta ctggtaacgc tacaggaaaa tcacgggctg acctatgtgc tgatttcaca
    13981 caatgtctcg gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat
    14041 tgtagaactg ggcgacgcgc aacaggtgct aacggctccg gcacatccat acacccgatt
    14101 attgctggat tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa
    14161 aacggatctg ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc
    14221 ccttgcaacc agcggatgtg aagtccggca atcattagca ataagggagg acggacgtga
    14281 gctccggtgc tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg
    14341 acgcgtcatc cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc
    14401 agtacctgag aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag
    14461 gcatcaatac ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg
    14521 gcatccactt tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtaaatccc
    14581 gcttccccca gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc
    14641 agttcttcag ggttagttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt
    14701 tggttcagca caccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc
    14761 cagtgtgcct gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga
    14821 aattatagac tacgtgagaa acagcacgag gataagcagg atattccgaa taaaaatccg
    14881 gcagaaatat tacttctgca cttaaattaa atttccacaa tagataaccg ggaatatata
    14941 acccaagaga tattgcatta atattaatca actcaccaca ataataatac ctatcaatat
    15001 catgaggtta aaatataaaa atcatcaacc aggactaatc ttaacaacga agcggcaaat
    15061 atttgccatg ctgaatgtgc tttataaatc tgcgatccgt agcagacacc ataaatacac
    15121 agacacggag aatcactatg tttacttatt atcaggcaga aaattcaaca gcagaacccg
    15181 ctctggttaa tgccattgag caaggccttc gggcagagca cggtgttgtc actgaagatg
    15241 acattctcat ggagctgacc aaatgggtgg aagcttctga taacgacatc ctcagtgata
    15301 tctaccagca aacgatcaat tatgtggtca gtggccagca ccctacgctt taaggtgcta
    15361 tgcttgatcg gcaacctaat ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg
    15421 tccttaaaac tagcaacagg attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca
    15481 tattcttgga ctggaccata aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa
    15541 atccagcgtg gtaaataatc caaccggcag aaaacgcccc gctgaaaagt aattcataac
    15601 catcagtcct caatgacgat taaacaccat tgcctgcgca atggtgtttt tgtttttatc
    15661 tgctttatac ttggggccga cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg
    15721 gatggatatt caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct
    15781 ttatatccct tacgctggcc ctgtactgct ggaatttccg ttgttgaata aaggcagtgc
    15841 cttcagcatg gaagaacgcc gtaacttcaa tctgctgggg ttactgccgg aagtggtcga
    15901 aaccatcgaa gaacaagcgg aacgagcatg gatccagtat cagggattca aaaccgaaat
    15961 cgacaaacac atctacctgc gtaacatcca ggacaccaac gaaaccctct tctaccgtct
    16021 ggtaaacaat catcttgatg agatgatgcc tgttatttat accccaaccg tcggcgcagc
    16081 ctgtgagcgt ttttctgaga tctaccgccg ttcacgcggc gtgtttatct cttaccagaa
    16141 ccgccacaat atggacgata ttctgcaaaa cgtaccaaac cataatatca aagtgattgt
    16201 ggtgactgac ggtgaacgta ttctggggct tggtgaccag ggcatcggcg ggatgggcat
    16261 tccgatcggt aaactgtcgc tctataccgc ctgtggcggc atcagcccgg cgtataccct
    16321 tccggtggtg ctggatgtcg gaacgaacaa ccaacagttg cttaacgatc cgctgtatat
    16381 gggctggcgt aatccgcgta tcactgacga cgaatactat gaattcgttg atgaatttat
    16441 ccaggctgtg aaacaacgct ggccggacgt gctgttgcag tttgaagact tcgcacaaaa
    16501 aaatgcgatg ccgttactta accgctatcg caatgaaatt tgttccttta acgatgacat
    16561 tcagggcact gcggcggtaa cagtcggcac cctgatcgca gcaagccgcg cggcaggtgg
    16621 tcagttaagc gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc
    16681 cgaaatgatc atcgcccaga cccagcgcga aggattaagc gaggaagcgg cgcggcagaa
    16741 agtctttatg gtcgatcgct ttggcttgct gaccgacaag atgccgaacc tgctgccttt
    16801 ccagaccaaa ctggtgcaga agcgcgaaaa cctcagtgac tgggataccg acagcgatgt
    16861 gctgtcactg ctggatgtgg tgcgcaatgt aaaaccagat attctgattg gcgtctcagg
    16921 acagaccggg ctgtttacgg aagagatcat ccgtgagatg cataaacact gtccgcgtcc
    16981 gatcgtgatg ccgctgtcca acccgacgtc acgcatggaa gccacaccgc aggacattat
    17041 cgcctggact gaaggtaacg cgctggtcgc cactggcagt ccgtttaatc cagtggtatg
    17101 gaaagataaa atctacccta tcgcccagtg taacaacgcc tttattttcc caggcatcgg
    17161 gctgggtgtt attgcttccg gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag
    17221 tgaaacgctt gctcagtatt cgccgctggt cctgaacggc gaaggtctgg tactaccgga
    17281 actgaaagat attcagaaag tctcccgcgc aattgcgttt gcggttggca aaatggcgca
    17341 gcagcaaggc gtggcggtga aaacctctgc cgaagccctg caacaggcca ttgacgataa
    17401 tttctggcaa gccgaatacc gcgactaccg ccgtacctcc atctaagcct gcgcccggta
    17461 gtgaaggcta ccgggctatt tccctctccc tttttcagat ctcatccata ctgggtagtg
    17521 gcgaataaat ctcatttgcc tcacctgcta tgcagaacat catccgaaaa ggaggaacta
    17581 tgaaggctgc agttgttacg aaggatcatc atgttgacgt tacggataaa acactgcgct
    17641 cactgaaaca tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc
    17701 ttcatgttaa gaatggcgat tttggtgaca aaaccggcgt aattctgggc catgaaggca
    17761 tcggtgtggt ggcagaagtt ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca
    17821 gcgtggcgtg gttctacgaa ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa
    17881 cgctctgccg ttcagttaaa aatgccggat acagcgttga tggcgggatg gcggaagagt
    17941 gcatcgtggt cgccgattac gcggtaaaag tgccagatgg cctggactcg gcggcggcca
    18001 gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taaactgtca aaaattcgtc
    18061 ccggtcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc ctgcaatacg
    18121 cgaagaatgt ctttaacgcg aaagtgatcg ccattgatgt caatgatgag cagttaaaac
    18181 tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac gccgccaaaa
    18241 ttgtgcagga gaaaaccggt ggcgctcacg ctgcggtggt aacagcagtt gctaaagctg
    18301 cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc
    18361 cgccagagtc tatgagcctg gatatcccac gccttgtgct ggatggcatt gaagtggtcg
    18421 gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc gccgaaggca
    18481 aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc tttaccgaga
    18541 tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgccgttaa gaggcctttg
    18601 ctgcgagtgc cagtcctggt gtcgcagcat cgcactctct ccgacattaa atggctaaat
    18661 ggattattgt tccttcctac gcaacaataa tgatagtgtt cctgatgaag gaacactact
    18721 gagagtaaag acactgatga aaatggccaa tcatccccgc ccgggggaca ttattcagga
    18781 atcactggac gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg aaattgctcc
    18841 ctcaacggca agccgattgc tgaccggaaa agcagctttg acaccagaaa tggcgattaa
    18901 actttccgtg gtgatcggca gttcgccgca aatgtggctg aatctgcaaa acgcctggag
    18961 tctggcagag gcagaaaaaa cggtggatgt gtcgcgacta cgccgtctgg ttacgcaata
    19021 aaaaaatggc gcttcttaaa gcgccatttt cagcttaaag ttcaaaagag attatatccc
    19081 ttcttcactc tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg
    19141 tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc cctttaatcg accctttcac
    19201 ccagaatgcc atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc
    19261 gtggatcagc aggctgtagc gaacaacctg catcgggaag tactgcgaaa agtacggacg
    19321 caagataatc accccggtca ccagcagcac gaaaatcatg ctcatgatcg accagaacat
    19381 catcttttgc ccggcgttgt acttaccgac atccgccact ttatgctcat tgcctttcaa
    19441 tacttcgata atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac
    19501 aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca atgccgaaaa acgggtgcaa
    19561 aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa
    19621 gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc
    19681 gcgatcaata aatttggtgc gcacaatcat tttcgactta ctcatgatga tcctcctcgt
    19741 catcgtccac ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag
    19801 tggcaataaa gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg
    19861 tgtcgatctt cggatctttc ggcagaccgt gatacagctc cggctgatcg gcgtgatgca
    19921 gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca gcatgttcgt
    19981 aaccacgcgc tttcagtttc gccacgcgct gttccgccag ctccagcatc tccttcttgg
    20041 tgccgaagtg gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc
    20101 tgacgcgatc gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg
    20161 gaatattaaa cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga
    20221 aatcgacaat cccgttagcg tactgaatga ttgcaccagc agacgggcac gccttcaggc
    20281 agccgggatc ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt
    20341 tctgttcggt ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt
    20401 tatcgtaaac cccgacgcag tgccccactt catcacggat gtcgttccac tccgaacacg
    20461 ccacctgaca ggctttacag ccgatacagg tggaaacgtc gataagtttt gcgacttctg
    20521 ctttgtaatc acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa
    20581 tgtcctgcgt ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga
    20641 acgctttata ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag
    20701 cgatataacc tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt
    20761 caacctgctg accatttaca ttcagtagtt tcagacgacg cgttaccaca gccaccgcgc
    20821 ggataaagcc acgcttgctt gagacagtga cacgatcgcc attattaatg cctttcgccg
    20881 ccgccagcgt ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg
    20941 cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc acatacggga
    21001 actgctcttt tttacccatc cgcagtgcgt cttgttcata cagacgaaca accgggttag
    21061 acaccacgtt cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc tcgtagtgtt
    21121 ccgggaacgg accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct
    21181 gcatgataaa cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca ggaatatcgt
    21241 tacccgtcca cttgctgccg ttccactgga tcagcatccg tttcggatcc cacggtttac
    21301 cgttgatatc cgccgaagca cggttgtaca gcacgcgacg gttgagcggc cacgcccagg
    21361 cccatcccag cgtattcccc agaccggacg ggtcggagtt atcgcggtta gccatctggt
    21421 tgccctgctc tgtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat
    21481 cacgcagatg cgcaaagcta ctcagcaact gacctttctt cgcaatcagc acgccattag
    21541 catcatagag atcttccagc gcatagccgt tgttctcttt agctacttcg tcagattgcg
    21601 gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag cggttctacg cctttaccac
    21661 cttcggcctg gtacagctcg cgcaggtgat ggtagatacc cgccagaatt tcaccgtcgt
    21721 tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca cagcagccag cgaccggagt
    21781 tagcaataga accatcttct tcagcaaagc aggtcgaagg cagacggaat acttcagtct
    21841 gaatagacgc cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gttgaggttt
    21901 cagtcaccag cggatcgata accaccatgt acttcagctt gcttaggcag ctcaccactt
    21961 tgtttttgtc cgggaaggac gcaaccgggt taaagccctg gcagaaataa ccggtgactt
    22021 tgccttcatc catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca
    22081 gccagtcata gccccagttg ttctctttct gcgcggcatc gccatagaaa gatttcatca
    22141 ggctaacgaa gaacttcgga tagttgctcc agtagttcac ctgaccagcc agcgtcgctt
    22201 tcggcgtgtt cgcttccaga tacgactgca aatcaacctg tttttctgac ggcagcgtca
    22261 gataacctgg caggctggta gagagcagac ctaagtcagt cagaccctga atgttggagt
    22321 gaccacgcaa tgcattcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga
    22381 tcatcgccat agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt
    22441 acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca
    22501 ggaagtcggc ttttggcgta ccgcagatgt tttcaacgac gtctggcgtg tagcgggaaa
    22561 cgtgctcttt cagcaggttc cacacacagc gcggatgagt cagtgtttca tcgcgtttcg
    22621 catagccgtt ttcatcgaac tgatagttcc aggacgattt gtcgtactgg cgtttttcag
    22681 cgtcataacc gctgaacagg ccatcttcga aagcaaaatc atcacgcacc agcaggctgg
    22741 cgttggtgta atgtttaacg tattcggcgt tgattttgtt gttttcgatc aggtagcgca
    22801 aaacgccaga caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca
    22861 cagaagcggt acgcgtaaaa cgaggatcga caacgatcaa ggttgcatcg ttgttgtttt
    22921 tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatta
    22981 ccattacgac gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa
    23041 atgttggagc aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg
    23101 ccagcatccc gagggagcgg gcaaatttct gtgtcagcat cccggtttcg ttgctggcac
    23161 cggaggcaca cagcataccg gtagaaagcc aacggtttac cgttacgccc tgctcgttct
    23221 tttcaataaa gttagcgtca cggtcagctt tcatcagctt tgcaatacgg gagaatgctt
    23281 cttcccagct aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca
    23341 gacggttttc gctgttgacg taatccagca aaccggcccc ttttgggcac agcgcaccac
    23401 ggcttaccgg atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg
    23461 cgccatcacc caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt
    23521 tacggatctc tttagcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg
    23581 gggcaaagcc caatgccgct accgttgttc cagccatacc gcccgcgcag attttaaaaa
    23641 attgtctgcg actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga
    23701 aactaacagt aaaattctga atctttttgc gcgaaatcaa aaagaacacg tgtttgccac
    23761 aaaataatcc gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac gcaccactgc
    23821 ctgacaggcc agttaaaaaa atgctataaa attcagctta atttttaacg gcaagagaga
    23881 caaaacagcg agcatgacac gacaaaaagc aacgctcata gggctaatag cgatcgtcct
    23941 gtggagcacg atggtaggat tgattcgcgg tgtcagcgag gggctcggcc cggtcggcgg
    24001 cgcagctgct atctattcat taagcgggct gctgttaatc ttcacggttg gatttccgcg
    24061 tattcggcag attccgaaag gctatttact cgccgggagt ctgttattcg tcagctatga
    24121 aatctgtctg gcgctttcct tagggtatgc ggcgacccgt catcaggcga ttgaagtggg
    24181 tatggtgaac tatctatggc ccagcctgac aattctcttt gccattctgt ttaatggtca
    24241 gaaaaccaac tggctgattg taccaggatt attattagcc ctggtcggcg tctgctgggt
    24301 gttaggcggt gataatgggt tacattatga tgaaatcatc aataatatta ccaccagccc
    24361 attgagttat ttcctggcgt ttattggtgc gtttatctgg gcagcctatt gcacagtaac
    24421 gaataaatac gcacgcggat ttaacggaat taccgttttt gtcctgctaa cgggagcaag
    24481 tctgtgggtt tactattttc tcacgccaca accagaaatg gtatttagca cgcccgtcat
    24541 gattaaactc atctctgcgg catttacctt aggatttgct tatgctgcat ggaatgtcgg
    24601 tatactgcat ggcaatgtca ccataatggc ggtaggctcg tattttacgc ccgtactttc
    24661 ctcagcgctt gcagccgtgc tgctcagcgc cccgctgtcg ttctcattct ggcaaggcgc
    24721 gctaatggtc tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg gttaaaataa
    24781 ttgccgggtc tacggcccgg cagttaatca gataaattcc gacaatatta aaaaataaat
    24841 ttcaaatacc agtaattcac
//
LOCUS       DAZGCS010000072        24472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGCS010000072 DAZGCS010000000
VERSION     DAZGCS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.072"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..1054
                     /gene="focA"
                     /locus_tag="ACHK01_003112"
     CDS             197..1054
                     /gene="focA"
                     /locus_tag="ACHK01_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO8098030.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1109..3391
                     /gene="pflB"
                     /locus_tag="ACHK01_003113"
     CDS             1109..3391
                     /gene="pflB"
                     /locus_tag="ACHK01_003113"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8098031.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3583..4323
                     /gene="pflA"
                     /locus_tag="ACHK01_003114"
     CDS             3583..4323
                     /gene="pflA"
                     /locus_tag="ACHK01_003114"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO8098032.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4405..4995)
                     /gene="ycaK"
                     /locus_tag="ACHK01_003115"
     CDS             complement(4405..4995)
                     /gene="ycaK"
                     /locus_tag="ACHK01_003115"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO8098033.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5095..6003
                     /gene="ycaN"
                     /locus_tag="ACHK01_003116"
     CDS             5095..6003
                     /gene="ycaN"
                     /locus_tag="ACHK01_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8098034.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(6004..7434)
                     /gene="ycaM"
                     /locus_tag="ACHK01_003117"
     CDS             complement(6004..7434)
                     /gene="ycaM"
                     /locus_tag="ACHK01_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8098035.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7644..8792)
                     /gene="ycaD"
                     /locus_tag="ACHK01_003118"
     CDS             complement(7644..8792)
                     /gene="ycaD"
                     /locus_tag="ACHK01_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8098036.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9107..9733
                     /gene="ycaC"
                     /locus_tag="ACHK01_003119"
     CDS             9107..9733
                     /gene="ycaC"
                     /locus_tag="ACHK01_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO8098037.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9769..10632)
                     /gene="dmsC"
                     /locus_tag="ACHK01_003120"
     CDS             complement(9769..10632)
                     /gene="dmsC"
                     /locus_tag="ACHK01_003120"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO8098038.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10634..11251)
                     /gene="dmsB"
                     /locus_tag="ACHK01_003121"
     CDS             complement(10634..11251)
                     /gene="dmsB"
                     /locus_tag="ACHK01_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO8098039.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11262..13706)
                     /gene="dmsA"
                     /locus_tag="ACHK01_003122"
     CDS             complement(11262..13706)
                     /gene="dmsA"
                     /locus_tag="ACHK01_003122"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO8098040.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(14006..14998)
                     /locus_tag="ACHK01_003123"
     CDS             complement(14006..14998)
                     /locus_tag="ACHK01_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8098041.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDARRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLLDAINFNPLAESVHKLSID"
     gene            complement(15068..15409)
                     /locus_tag="ACHK01_003124"
     CDS             complement(15068..15409)
                     /locus_tag="ACHK01_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8098042.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            15514..16035
                     /locus_tag="ACHK01_003125"
     CDS             15514..16035
                     /locus_tag="ACHK01_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO8098043.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLDQLRNNRGN"
     gene            16040..16462
                     /locus_tag="ACHK01_003126"
     CDS             16040..16462
                     /locus_tag="ACHK01_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098044.1"
                     /translation="MKTNTSDFTITISKNDQALIDNLKYLAEKHNNGRIAINLRHITG
                     VTIVTSYWHLYLIAKSVLENKDSVVNGRDIDTLPAQIKPIAYQLRNRFKNEGGIIFDF
                     PAAVAVSFSRGDILHSFRFALSGDETLWQLEDAGSSPA"
     gene            complement(16469..16660)
                     /locus_tag="ACHK01_003127"
     CDS             complement(16469..16660)
                     /locus_tag="ACHK01_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098045.1"
                     /translation="MSEDKKVVNLLSELSAALDNINPPRVKTTGDKDALNTEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            16798..17148
                     /locus_tag="ACHK01_003128"
     CDS             16798..17148
                     /locus_tag="ACHK01_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8098046.1"
                     /translation="MKLQRNSSQQRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVD
                     RLLHGQSFIKQADL"
     gene            17159..17437
                     /locus_tag="ACHK01_003129"
     CDS             17159..17437
                     /locus_tag="ACHK01_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098047.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARKTNGDEPYIEIICMDSEAEIQKAD"
     gene            17449..17691
                     /locus_tag="ACHK01_003130"
     CDS             17449..17691
                     /locus_tag="ACHK01_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO8098048.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            17688..17801
                     /locus_tag="ACHK01_003131"
     CDS             17688..17801
                     /locus_tag="ACHK01_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098049.1"
                     /translation="MSIFMSWIVLIISVVCAIGIVRIIHSVKKIERFFTGE"
     gene            17895..18305
                     /locus_tag="ACHK01_003132"
     CDS             17895..18305
                     /locus_tag="ACHK01_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098050.1"
                     /translation="MHAKEEGIIRALKEISKTENEVAKKAIANNHMDVATHTLIVARV
                     TAEAAEIIAKQDAELAVLRTQPVTGLDLSNTGRLIYTIGSELQRYTIIAGLQDKYLIT
                     PHPIRESEILTNLRLIERSQVAFIDDAQCTVFNA"
     gene            18329..18532
                     /locus_tag="ACHK01_003133"
     CDS             18329..18532
                     /locus_tag="ACHK01_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098051.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            18529..18795
                     /locus_tag="ACHK01_003134"
     CDS             18529..18795
                     /locus_tag="ACHK01_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO8098052.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            18792..19091
                     /locus_tag="ACHK01_003135"
     CDS             18792..19091
                     /locus_tag="ACHK01_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098053.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAALALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEVPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            19103..19363
                     /locus_tag="ACHK01_003136"
                     /pseudo
     CDS             19103..19363
                     /locus_tag="ACHK01_003136"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19414..19644
                     /locus_tag="ACHK01_003137"
     CDS             19414..19644
                     /locus_tag="ACHK01_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO8098054.1"
                     /translation="MANRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHELQQLIQQQNKLH"
     gene            19717..20082
                     /locus_tag="ACHK01_003138"
     CDS             19717..20082
                     /locus_tag="ACHK01_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098055.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            20089..22911
                     /locus_tag="ACHK01_003139"
     CDS             20089..22911
                     /locus_tag="ACHK01_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8098056.1"
                     /translation="MTAEYLRDWQQPRHAVGREGTGIPAPESALSSWLDAYRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDSTDGRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQGQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            22988..23947
                     /locus_tag="ACHK01_003140"
     CDS             22988..23947
                     /locus_tag="ACHK01_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO8098057.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVAPAIREVCGVNATFIADGVPQFALVNGLYAMDKE"
     gene            23952..24266
                     /locus_tag="ACHK01_003141"
     CDS             23952..24266
                     /locus_tag="ACHK01_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO8098058.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQDNQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         6636 a         6376 c         5799 g         5661 t
ORIGIN      
        1 atgcggaaaa aattcaactc atttgttaat ttttaaaatt tatttttatt tggataatca
       61 aatatttact ccgtatttgc ataaaaacca tgcgagttac gggcctataa gccaggcgag
      121 atatgatcta tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa
      181 taaagagaga gttagtgtga aagctgacaa cccttttgat cttttacttc ctgctgcaat
      241 ggccaaagtg gccgaagagg cgggtgtcta taaagcaacg aaacatccgc ttaagacttt
      301 ctatctggcg attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc
      361 aaccactggc acaggcacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt
      421 ctctctgggg ctgattcttt gtgttgtctg cggagccgat ctctttactt ccaccgtgtt
      481 gattgttgtt gctaaggcga gtgggcgcat cacctggggt cagttggcga aaaactggct
      541 aaatgtctat tttggcaacc tggtcggcgc actgctgttt gtacttttaa tgtggctttc
      601 cggcgagtat atgaccgcaa atggtcaatg gggactaaac gtcctacaaa ccgccgacca
      661 caaagtgcac catactttta ttgaggccgt ctgtcttggt atcctggcaa acctgatggt
      721 atgtctggca gtatggatga gttattctgg ccgcagcctg atggacaaag cgttcattat
      781 ggtgctgccg gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt
      841 tatgatcccg atgggtattg taatccgcga cttcgcatcc ccggaatttt ggaccgcagt
      901 cggttctgca ccggaaaatt tttctcacct gaccgtgatg aatttcatca ctgataacct
      961 gattccggtt acgatcggca acattatcgg tggtggtttg ttggttgggt tgacatactg
     1021 ggtcatttac ctgcgtgaaa acgaccacca ttaatggttg tcgaagtacg cagtaaataa
     1081 aaaatccact taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc
     1141 ctgggaaggt tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca
     1201 gaaaaactac actccgtacg agggtgacga gtccttcctg gctggcgcta ctgaagcgac
     1261 caccaccctg tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc
     1321 agttgacttt gacaccgctg ttgcttccac catcacctct cacgacgctg gctacatcaa
     1381 caagcagctt gagaaaatcg ttggtctgca gactgaagct ccgctgaaac gtgctcttat
     1441 cccgttcggt ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga
     1501 tccgatgatc aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtgttcga
     1561 cgtttacact ccggacatcc tgcgttgccg taaatctggt gttctgaccg gtctgccaga
     1621 tgcatatggc cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga
     1681 ctacctgatg aaagacaaac tggcacagtt cacttctctg caggctgatc tggaaaacgg
     1741 cgtaaacctg gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct
     1801 gggtcagatg aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggctaccaa
     1861 cgctcaggaa gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa
     1921 cggtgctgca atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga
     1981 cctgaaagct ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat
     2041 gaaactgcgt atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga
     2101 cccgatctgg gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta ccctggttac
     2161 caaaaacagc ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa
     2221 catgaccatt ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt
     2281 gtccatcgac acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa
     2341 caacgatgac tacgctattg cttgctgcgt aagcccgatg atcgttggta aacaaatgca
     2401 gttcttcggt gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt
     2461 tgacgaaaaa ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag gcgatgtcct
     2521 gaactatgat gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta
     2581 catcactgca ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct
     2641 gatggcgctg cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggcctgtc
     2701 cgttgctgct gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga
     2761 agacggtctg gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaatga
     2821 tccgcgtgta gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa
     2881 actgcacacc taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt
     2941 tgtgtatggt aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg
     3001 accgggtgct aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt ctctgacttc
     3061 cgttgctaaa ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt
     3121 tccgaacgca ctgggtaaag acgacgaagt tcgtaagacc aacctggctg gtctgatgga
     3181 tggttacttc caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat
     3241 gaaccgtgaa atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat
     3301 ccgtgtatct ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt
     3361 tattactcgt accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa
     3421 agcgtacaat aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc
     3481 agctatctat actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca
     3541 ccaacacggc ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc
     3601 attcactcct ttgaatcctg tggaaccgta gacggcccag gtattcgctt tatcaccttt
     3661 ttccagggct gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggatacgcat
     3721 ggcggtaaag aagttaccgt tgaagattta atgaaagagg tggtgaccta tcgccacttt
     3781 atgaacgctt ccggcggcgg cgttaccgca tccggcggtg aggcaatcct acaagctgag
     3841 tttgttcgtg actggttccg cgcctgcaaa aaagaaggca ttcatacctg tctggacacc
     3901 aacggttttg ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg
     3961 gtaatgctcg atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttcc
     4021 aaccaccgca cgctggagtt cgccaaatat ctggcgaaca aaaatgtgaa ggtgtggatc
     4081 cgctacgttg ttgtcccagg ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa
     4141 tttacccgtg atatgggcaa cgttgagaaa atcgaactcc tcccctacca cgagctgggc
     4201 aaacacaaat gggtggcaat gggtgaagag tacaaactcg acggtgttaa accacctaag
     4261 aaagagacca tggaacgcgt gaaaggcatt cttgagcagt acggtcataa ggtaatgttc
     4321 taatttcccc gtaaagcggc aactttattg agttgccgct tttttattcc gcgcttcatt
     4381 ctgctacagc gtttttaaca cattttatag tgcatctacc atatcccgta cctgagataa
     4441 taacgactga taatagctcg catgaagttc ttgcccatcg aataccactg tattacacaa
     4501 gaaagtgaca tcggcatctt caataccaag ataactgcac atatttttca aataatcgct
     4561 tatatttttt tcccagccca tctggacaaa tgattctttg tctcctccaa ccagcgccac
     4621 ccaacgaact ttattgaatg gtaatttgtg cccatctcca taagccagcc cattattcca
     4681 tactctgtca atatatcctt ttagcattgc tgggaagctg taccaccaga gaggaaaaac
     4741 caccactaac gtgtcatgtt caagcagctc tgaataaagc tgatgaacct ctggagaata
     4801 acgtttatcc atattcttcc agtctggttc atcttccgac gtcatcaccg ggttgaaatt
     4861 acgccgatat aaatcgagtt ccgtcacctg tattttccgc tccattgccc gctgatggat
     4921 cgcatcagca atatgtgcgg tcaatgaatc atgacgagga tgggcccata ccaaataaat
     4981 acgttcagac tgcataattt tacctccacg ttaagttctg gagcgcactt tacgcaggcg
     5041 gcataatcct gtaaacgtga tgattcagga tgagttaatg aaggattttc actaatgcgg
     5101 atgaatatgt ctgactttgc cactttcttt gccgtggccc gtaatcaaag ctttcgtgca
     5161 gcgggcgatg aattaggctt atcctcgtcc gccattagcc atagtattaa aacactggaa
     5221 caacgtctta aaattcgctt attcaatcgg acaacccgaa gcgtatcgtt aacggaagcg
     5281 ggttctaatc tctatgaacg attgcgccca gctttcgacg aaattcagat catgttagat
     5341 gagatgaatg attttcgtct gacaccgaca ggaacgctca aaatcaatgc cgcccgggta
     5401 gctgcacgta tatttttaat gcctttactg gttggtttca cacgtgaata tcctgatatt
     5461 aaagtcgagc tcacaactga cgattctctt gtcgatatcg tccaacaggg ctttgacgct
     5521 ggcgttcgac ttagtggcat cgtcgaaaaa gatatgatca gcgtggcgat cggcccacca
     5581 gtgaaattat gtgtagcagc aacgccagag tactttgcaa gatatggcaa accccgccat
     5641 cctcatgatc tgcttaatca tcaatgtgtt gtttttcgtt atcccagcgg caaacctttc
     5701 cactggcaat ttgcaaagga gctggaaatt gctgtcgcag gaaatatcat tctggatgat
     5761 gttgatgctg aactggaggc cgttttgatg ggtgctggta tcgggtatct gctctatgaa
     5821 cagatcaagg agtaccttga tactggtcgt ttagaatgtg tactggaaga ctggagcaca
     5881 gagcgttcag gcttccagat ctattatcca aaccgccagt atatgtcctg cggcttgcgc
     5941 gcatttctcg attatgtaaa aacgggtcag atctgccaga gtcagcgtca ccgaccacaa
     6001 taattattta ctattcgctt tacgcgccag cagcgggaat atcagtccta atcctaccag
     6061 cacaaacggc gttgcaacat tcagcgccaa ctggaaggtc cactctgcag tgaaggcttc
     6121 cattttcggg aagatccccg tcaagcaggc aaaggcggta aaggcaaaac accaaatccc
     6181 gacggtcatt gccagaggct tattacgaat aaagacatat tctggtttat atttctgcgc
     6241 caggcgaacg actgcaataa atgcaacaaa tacccataaa taacgcagcg gcatcactac
     6301 cgagttaaga ttcaacagcc atttatagag attgttcata tcaccgatgc cgagagtcgg
     6361 cagcataatc agaattgcca ccagcaccag ggtcagaaaa tagccattaa cgggcgtacc
     6421 agaagcgttg gtacgacata aactggctgg aatatatttg ctgtcagcat cacctaatag
     6481 cactttaagc ggggcatcaa tcgagaatac cagcgcagct acttgtccca gggtattcgc
     6541 aatggcgtaa atcaccatta aagtattacc catgttgtaa tactcgccca gcttctgaaa
     6601 ggcgtaatat tgaccgttag tcattaagtc atccgggata ttacgcgagt caaacatcat
     6661 ccccatcgcc agtgagccca gaatggcaca aaccgcaacc atcaccgcca ggcatagcat
     6721 cccttttgga aattcttttc ctgggttgcg cgtttgatta acgtaaggag aaatcttctc
     6781 tgctccgcca accgcgaaaa ccagcattga aatagtggta atgtaggtaa agtcgatatg
     6841 aggaatgaac gtttcccagg taatgtttgt ggtcgcaata tgcacttcag taatcgcagg
     6901 cgcggttacc gccatcgcca catacaggag cgacataaca aacatcgcaa tccctgccac
     6961 agaaccgacg attttcagcg atttcatacc gcgtgaagca acccacataa agaagataaa
     7021 cagcaccagc gttaacccct gtaacgctac gactgaatat tctttgatta gcgaaccgtc
     7081 gcctttcatc gcccaaccga gcgcaatcag aattgcctgg ggtttttgtg ccagataggg
     7141 aatatgcacc acccagtagg tccacgcggc gagataagcc agtccgggtc ccatcgtgtg
     7201 tttaatccag gtactgaccc cgcccttccc atctttgaag gtcgagccta actgaccaac
     7261 aattagcgca taaggtgtga aatagagtgc aaagataaac acccatgaaa aaacaaccac
     7321 cagcccctgg ttggcatagt tattaacaac gttgccaaaa ccccagacag tgataaaaga
     7381 catcagcgca atgttgtacc atcgcaactg tttttcctga acattcccag ccatagacaa
     7441 atccttcgcc aaaattaaag gggcatctgt aaaacgaggg attatgcgcg gcaaataaga
     7501 agtgcggaaa tagcgaaatg caaaattata acctgctgca cattttattt ttaaacgaat
     7561 aaaatttgga ataaagcaag tcagatagaa gaaacaaaaa agcagagtgt gaaacagtaa
     7621 gttcacactc tgcttgaatt catttacacg tgagcaacgg gtttcggcgt atgaccggcg
     7681 ttgcgcagca gcatcagcaa atagataaac gatacgctgg cgatcatgat aaacaataaa
     7741 ttatcggaga aattctgcat tagcatagcg gtaaatgacg ggccaagcag acttcccaca
     7801 gtataactca acagtaaggc ctggttcatc gccaccagtt gatgatgttc aactttctcg
     7861 caagcccatg ccatcgccac cggatatagc gtaaagccag cggcaccgag gatgaataac
     7921 gctggggcca tcgccgcctg gctaagcatc gcgatactgc cgagaatgac gacaaagacc
     7981 tgaacacgca acaccagcag tcgaccaaac ttatccgcca gacgtccaat cggccattgt
     8041 ccaaggatac ccgcactgac cagtaccgcc atccagaaac caatgctggc attgctcacc
     8101 cctttgtgat tgaggtacag cggcatcagg ccatatagag atcccagaac gattcctgag
     8161 ataatgcagc cattcacgcc aagccgcgcc tgacggagtt ttagcattga cgtaattgac
     8221 gtcgaatcat ggttttcagc ctgctgattc agcacacgcg taaacaacag cggtaagatc
     8281 cctgccaacg tcaaacctgt aacccacggc aatacggaca tcagctcggt tgaaactttg
     8341 ctgaccagta actggcctaa aaacgttccc acgtaataaa ccatcatata cgcagcaagc
     8401 aaacgcccac ggttacgtga cgtcccactg cacatcagcg cgctctcaac aaccacccaa
     8461 atcatggcac agccgacgcc cgcgacaaaa cgccaagcca accagctcca gaatccaatc
     8521 atcaggccaa ggccggcaca gccagcggca aaaatgaagg aggccagata atagctgcgg
     8581 ttaaagccaa tgcgcttaat gacataccct gtcagcaatg taccgacaag gttgccggta
     8641 aaataggatg agctgacaac gcctacctgc catgtggaca tgtgttcctg ggcgagccaa
     8701 agcggcacga gtgtatttaa caccgcgatc gccagagtca acaaaagcag gccagacagc
     8761 aaaagcatga caggccgggt atacgtggac atgaattaaa accgtgagga agttcaaatt
     8821 tcatgcgcat catgccaccg ccaaaaacat tgtcaatggg tgaaaatacg cgcatatcgc
     8881 agcggcggta ttatcgattt atattactca ttcataacgc taatgctgaa gagacgatta
     8941 atctcatctg attgttttta ttgatctaac acaaatttca tacgcttcgt tcagtcgaaa
     9001 aatttcatgg tcacagcaaa ctcatatttc ccgtctatgc tttcagagca atgcatcgcg
     9061 tctgaccgat ttcagacgta ccactgactc tcgaaaggag aagaggatga ccaaaccgta
     9121 tgttcgtctt gataaaaatg atgctgccgt tttgcttgtt gatcaccagg ctggtttact
     9181 ttcccttgta cgggatatcg aacccgataa gtttaaaaat aacgtgctgg cgctgggtga
     9241 cctggctaag tatttcaact taccaactat tcttaccacc agttttgaaa ccggtcctaa
     9301 cggtccatta gttccagaac tgaaagcaca attccccgat gcaccttaca ttgctcgccc
     9361 gggaaatatt aacgcctggg ataacgaaga ttttgtaaaa gcagtcaaag cgacaggtaa
     9421 aaaacagtta attattgccg gtgtggtaac cgaagtttgc gtggcattcc cggcgctttc
     9481 agccattgaa gaagggtttg acgtctttgt cgtgactgac gcgtcaggga cctttaatga
     9541 aattacccgg cattcggcat gggatcgcat gtcgcaagca ggtgcgcaat tgatgacatg
     9601 gttcggcgtg gcgtgcgagc tgcaccgtga ctggcgtaat gatattgaag gactggcgac
     9661 gttgttctct aaccatattc ctgattatcg taatttgatg accagttatg acacgttaac
     9721 gaagcagaaa taaaagagaa ccggggccga aaccccggtt gttactgatt agcttgcgac
     9781 ggccatcccc acggtcatat gcaaaccata gaatacgccg cgaccgatta attcccctgc
     9841 cagaagcaga atgaatgaga cggatagtag cggaaccgca ggctgataac cttttagctg
     9901 tggtgcaatc cacaagcaca gggcaacggc caaaagcacg atccgccagg acatcagcgc
     9961 accatagtcc ggcaccagcg ctgcggcctg ctgcacagaa ctatgaatag ttgccagctc
    10021 tgtgccctgc atcactgaca tcacaccact caccaccagt gccagtacag aaatggctgg
    10081 cagcagacgc atcgcccagc catcgacgcc agccagactc agcaacaggt aacccagtaa
    10141 cgggccgccc atgaacatcg tcaggaagaa gcccatcggc gtccagatgc tgtaccaggt
    10201 cggaacggta tcaatgctgt tatacacacg caccatcatc cagacaaaga cgacgccaag
    10261 aaccatcgtc accaccagcc atagcgtacg caatgccggt gacagctttt tcagcattgc
    10321 cagcaaccag ccgatgccgc ctacagcaaa aaagatcgaa ccgctggcga tttcgttact
    10381 gagtgctgaa gcccctaccc ggttgagcga gttaaaggcg cgcatcggtg aaccaagatg
    10441 gagcatagag gcgataaagc caatgcccat cagcacccat aagccaaaca tgcaggcgat
    10501 aacacgctgc tgggcttctg ctcgcaggtc gcctttgagc agcgccaaag ccagaacgat
    10561 aaaaccaccc gctacacatt gcccgaagac cgtgaagatc atcagcggcc attcatgcca
    10621 tccacttccc atctcacacc tccttcgggt ttgccaggta gccagtggta tccccggtcg
    10681 ggcggctgtt ggcattgggt ttgatcacaa tattcggttt ggtaaagtga gctcgcggca
    10741 acggcgcaac ggccgccaga tcgccatgtt ttttacgcag ctcgtcgata ggaccgaaat
    10801 ccagcgcccg cagcgggcaa gattcaacac agatcggctt tttaccctcc gcaacacggt
    10861 cataacaacc atcgcatttg gtcatatggc ctttcgtttc gttatattgc ggtgcgccat
    10921 acgggcaagc catatgacag tagcggcagc caatgcatac atcttcatcg accacaacaa
    10981 aaccatcttc gcgtttatgc atcgcaccgc tcgggcagac tttagtacaa gccggatctt
    11041 cgcagtggtt acatgaaatc gacagatagt aggcaaacac gttctggtgc cagacaccgt
    11101 tatcttcctg ccagtcgccg ccagcatatt cataaatgcg gcggaagctg acttctggcg
    11161 tcaaatcttt gtagtcttta caggccagct cgcaggtttt gcaaccggtg caacggctgg
    11221 aatcaataaa aaatccatac tgggttgtca tcggttactc cttacacctt ttcaacctga
    11281 acaaggtttg tatgtgacgg attcccctta gcgagaggag acggacgttg agtggtcagt
    11341 acgttaatac aaccgccctt atcgacacgt tttgcatccg ggtcatacca ggcaccttca
    11401 cccagtgcga ccacacccgg catcattcgt ggcgtcactt tcgcctcaat atgaacttca
    11461 ccacgatcgt taaagatcct gactttatcg ccgttgttga taccgcgttt ttgcgcatca
    11521 agcgggttga tccacatttc ctgacggcaa gccgctttca gcacatcaac gttgccgtaa
    11581 gttgagtgaa cgcgagattt atagtggaaa cccgtaagct gcagcggata ctgtttgttc
    11641 agcggatcct gataactttc aaagcccggc gtgtagatcg gcagtggatc gatcacatcg
    11701 ccttctggca attcccaggt agcggcaatg tcagccagcg cctgcgaata aatctcaatt
    11761 ttgcccgatg gcgtagtcag tgggtttgcc tgcggatctt cacggaaggc tttataagca
    11821 acgtgatgcc cttgtgggtc gcgcttttta aagatcccct gcttgcggaa ctcttcaaac
    11881 gttggcagtt caggaatcgc ttcccgcgac tgggcataca gatgccgcat ccactcttcc
    11941 tgtgtacggc cttcagtaaa ctgttgctca acgccaagac gttttgccag ttcgctggtc
    12001 atttcataga tggtcttaca ttcaaagcgc ggtttaatca cctgatcgtt gaaaatcacg
    12061 taagacatat tcccgcagga tgcatccagc gcaaagtcca tctgttcgga agcggtgcag
    12121 tcaggcagca ggatgtcagc atatttcgcc gatgaggtca tgtggcagtc gataaccaca
    12181 atcagctcgc acttcttatc atcctgaagg atttcatggg tacggttgat ttcagaatgc
    12241 tggttaatca ggcagttacc ggcatagttc cagatcattt tgatcggcac atccagctta
    12301 tctttcccgc gtacaccatc acgcagcgcc gtcatttccg ggccacgttc aatggcatcg
    12361 gtccacataa acatcgaaat gctggtctgg atcgggtttt ccaaggtcgg catacggaca
    12421 aacggtaagc tgtatgaacc ttcacgcgcg ccgctgttgc ctccgttaat accaacgtta
    12481 ccggtcagaa tcgccagcat cgagatagca cgggttgcga tttcaccgtt agcgtgacgc
    12541 tgcgggcccc atccctggct gataaacgcc ggtttggtac tgccgatttc acgagccagt
    12601 ttgatgattt tgtctgccgg aacaccggtg atttgtgagg cccattccgg tgttttagcc
    12661 acgccatctg gcccttcacc cagaatataa gctttatagt ggccattttt cggcgcactg
    12721 gctggcaggg ttttctcatc gtagccaacg caatatttat cgaggaatgc ctgatccacc
    12781 aggttttcag tgatcatgac gtacgccaga ccgttaacca gtgcggcatc tgttcccgga
    12841 cgaataggga tccactcatc ttcgcgcccg gcaccggtgt cggtatagcg cggatcgatg
    12901 atgatcatgc gggcattaga tttctggcgt gcctgttcaa gatagtaagt caccccaccg
    12961 ccactcattc gcgtttcgcc agggttatta ccaaacagca ctaccagttt actgttttcg
    13021 atatccgacg ggctgttacc atctgcccag ccgccgtagg tatagttcaa accttccgca
    13081 atttgcgcgg aagagtagtc accgtaatgg ttcagataac cgccgcagca gttcatcagg
    13141 cgcgcgacca gggtatttcc cgggggccag gagcgggtca tggtgccgcc cagcgtaccg
    13201 gtgccatagt tcagatagat ggattcgttg ccgtactctt tgatcaggcg ctgcatattg
    13261 gtcgcgatga tgtcgtaggc ttcttcccag ctaatgcgct caaatttgcc ttcaccgcgc
    13321 gccccgactc gtttcatcgg atatttcagg cggtccggat tatagacacg gcgacgcatg
    13381 gaacgtccac gcagacaggc gcgaacctgg tgcaggccgt cgtaattgtc atcgccggta
    13441 ttgtccgttt cgacatattt gatttcaccg tccacgacgt gcatacgtag cgggcagcga
    13501 ctaccacagt taactgtaca ggcgctccag ataacctttt cgtctgattt tgttggaatg
    13561 gcgctatcga cagcgtgcgc aatccgacta aaaggtaatg ttaatgcgct gctggccatt
    13621 gccaggccgc cgatcgctgt cgtttttacc aaaccacggc gactcacctc agcagccaat
    13681 accgcatcag ggattttcgt tttcataatg gctcactcaa gcttgctcac gattgattcc
    13741 agtaacaaac cgtattgtgt aattatttct atagcagctc gctatatagg cttgtataca
    13801 tcgaataatt tgaaaatcaa agagataggt agattttcac tcgaaagtag tattacgtgt
    13861 aagtgaggag taattattgt tcggtatcaa agggagacaa aaaaagcgcc cgcaggcgct
    13921 ttaacgtttc ttgccccccc tgatgattta cttatttttc aacgaataag ccaaaacagg
    13981 gggaacaaat taacacaaat tataactaat cgatggacag tttatggaca ctttcagcca
    14041 gcggattaaa gttgatcgcg tccagtaaaa agtccggcga aaaatgagcg tacgtcattg
    14101 tttgctgaat cgttgcgtgc cccaggattc gttgcaacgt aacaatattt ccgccattca
    14161 tcataaagtg tgtggcaaat gtatgacgca tgacatgcgt agcctgcccc ttcggcaaat
    14221 ccggtttcac ctcctgaaga atctcccgga acttcagata atcaacctga tacaataacc
    14281 ccgttcgacg ggttttaatc agaggcacga ccgaatccgc cacaggaacc gaacgggatt
    14341 ttccgttttt ggtgttaaag aacgtcaccc ggttgccaac gatatgctct ccacgtagtt
    14401 ccgatgcttc actccatcgg gcaccagtag aaagacacag aatggcaaca cgacgcgcat
    14461 caccctccag tcgctcaaga agccgttcaa tctcatcatc agaaagaaag gccatctctg
    14521 tgttctgaac tttcagtttt cgtataccgc gtacagggtt cgcgttgtga aagacctcag
    14581 cttcaatcag caccgtgaac atggtagaca gtacgcataa gtcacgatta atactggacg
    14641 gcattaatcc ggcctgtaat ttctctgacc gatattcaag cataaatttt cgcgtcatct
    14701 gactggcgcg aggatctccc atttcacgaa caaccttccc tagcctaacc ctgtagctat
    14761 ccccgtaagc ctgattacgc ccatcaagca tccaccacgc atcaagcagc tctgataatc
    14821 gccgctgatc agctggctta gcctgccatg gcttgtcatg aaagttctgt aacacatact
    14881 tctcaaactc ctgagccttt gatttcaggg caaagatttt tcgaatccgc tttccttccg
    14941 ccccctgcgg tctgacatcg acctgataac gtccgtcttt gagttgctta atcgacatga
    15001 ttagcccctc caacggataa attaactaaa cactccttgc gatatgtaat tcgctcaatg
    15061 tgtagagtca gccaacattg cggcctgatc ggggtgattt ttgggtaata ccgattgaaa
    15121 cctgatcgcc ctccgactga atagatccat caagagagag agccggagca atctgcccgg
    15181 cgacaggtag cgtcttatct tttattagcc acagcatgta tttttcaaag cgatcgactt
    15241 gcagaaccgc ctcaacgatt tttgctctag cgttcttttg ccccgattcg taattcctta
    15301 tggttgccaa agacaaccca gtaatatccg caaactgctt ttgtgttaac ccttccgcct
    15361 tacgtatctg acgtaacttt tttgcgtagt ctattgacaa gataaccatt tgagtattaa
    15421 cctctcgcac aaagataacc ttttggttat ctttacaagc caaaacaaac catcacaagg
    15481 cgcgacaagc gcaccaacgc aagaggataa caaatgcggg acttcattgc cgataaagag
    15541 ttatctgacc accaaccact tcaggaaagt gacgcactca gcggatccat tgatgccgcc
    15601 aacgacgacg agcacgaaga aaacaagaaa aagcgttcct acaaaaaagg agcgactttg
    15661 cggctggatg gtccgatcgc cgggctttgc tctctggaaa aaggcgcggc atatattggg
    15721 ctaacaaaat ctgcactgcg cgttgccatt catcgcggtc aaatgccggg acacaagacg
    15781 cgcacaaacc cggaagatga aaactcagac ggagtatggt ggtttaacgc caaagagtgg
    15841 gacaagttgg ctgatgagct accagagcac gagcccccag aatggcacaa ctggaaaagt
    15901 tactggacgt atgaccgcca gaaaaggaag ttctctccag ctaataaaga agactgccag
    15961 accattaacg gtaagcgagt ttatacaggc agaagctcaa aactggacca actcagaaac
    16021 aacagaggca actaatcgta tgaaaacaaa cacgtcagat ttcacaataa ctatttcaaa
    16081 aaatgaccaa gcattaattg ataacctgaa ataccttgcg gaaaaacata ataacggacg
    16141 aattgcaatt aatttacgac acatcacagg agtaacaatt gtaaccagtt actggcatct
    16201 atatctaatc gctaaatcag tacttgaaaa caaagattct gttgtcaatg gaagagatat
    16261 agatactctt cctgcacaaa tcaaaccgat agcctatcaa ttgcgtaatc gttttaaaaa
    16321 tgaaggtggg attatatttg actttcccgc agctgtagcc gtgagcttct ctcgggggga
    16381 tatacttcac tcatttcgtt ttgctctatc aggtgacgag acattatggc agctagaaga
    16441 tgccgggagc agcccggcat aaatcaaatt aaaaatcaac cagttgtgtg acaggtgggt
    16501 atttacgctg aattttgtca gttactaacg ccagtaatat atgaatctct gtgtttaatg
    16561 cgtctttatc acccgtcgtt tttaccctgg gagggttaat gttatccaga gcagcactaa
    16621 gttcactcaa caaattaaca acttttttat cttctgacat gaaaacctct taacaattta
    16681 attaaacata agccaggacc tgaaccggcc agtccaagtc ctagacacac aaaaaaaata
    16741 ccacaaatca atacaaggtg acacgagtac accttaccga aggtacagag ttttaatatg
    16801 aaactacagc gcaattcttc acagcagcgc ttccgcaacg gtgcggaacg ccatgctaac
    16861 cgtttcgcta ccagtgcatc acgtagcaac tctcgctaca gcctgagcga aacacacgca
    16921 acgccggatg gctacccagt aaaacaaatt ggtgaacaca cttggctgat tgaaaaagca
    16981 ggaatcgtgg ttcacagatg ccagcgcaac ccatttaccg gaaaccgcat ttttgcactg
    17041 agcaacggcg acaatcagtt cgggcaggat ttcacattat acgaagcact tcgcacggtt
    17101 gatcgtctgc ttcacgggca aagttttatt aaacaggctg atttataaca ggtgctttat
    17161 gaccaaagag catgcacaag gtgtatttat ccgttttatt gattttcgcg gtgaactgtt
    17221 attacgcgca tcagccattg acggagttgt tccatccgaa aaaaatgcag ctacttacgt
    17281 ttatctgaac ggcacgcgcc tgaccgtaga acttccgtac cagactgtac acgaaatcat
    17341 tagcgaagcg gaaaaagcac gtaagactaa tggcgatgaa ccctatatcg aaattatttg
    17401 tatggattca gaagcagaaa ttcagaaagc agattaaagg gcgttgcgat gggcaaagaa
    17461 tataaaactc tcattaacaa agcacttgag cgtttttatt ttcgcttaag tgcatcaggc
    17521 gctcatgctg aacgtgcagc ccgtgactca ttgaccaggg caatccggag tctgtatgac
    17581 gtggcttttt acgctgatga tctggatgca cttaacgaac tttccgagct gatctgtgcc
    17641 gcagaatgcg gggaacatat tgaaccgtat aagctgggga atatcgcatg agtatattta
    17701 tgtcatggat tgttctgatt atttcagtgg tctgcgcaat tggcattgtg cgaattattc
    17761 attcagtaaa aaagattgaa cgttttttca ctggtgaata acgatacaaa taaaacatca
    17821 aattaaataa gaaaacgtga aaaccatccg tattaacgga ggtattcgca cacgcaaata
    17881 acggagatac aaaaatgcac gcaaaagaag aaggtatcat cagagcactg aaagaaattt
    17941 caaagacaga aaacgaagta gcgaaaaaag ccatagcaaa taatcatatg gacgtcgcaa
    18001 cccacacact gatagtcgca agagtcacgg cagaagcagc cgaaattatc gcaaaacagg
    18061 atgctgaact ggcggttctc agaacacaac cagtcaccgg actggatttg tctaacaccg
    18121 gacgccttat ttacacaatt ggctcggagc tacagcgata caccattatc gccgggttac
    18181 aggataaata cctgatcact cctcacccca taagggaatc agaaattctg acaaatctcc
    18241 gcctgataga gcgctctcaa gtcgcattca ttgatgacgc acagtgcacc gtatttaacg
    18301 catagagtta ctggacaaag ggggcgcaat ggcaattaag cattttcccg tcgttcgctt
    18361 tacctccaga gggcgcgaat acgaggtcga cgaacgcctg attaccacta tcgacaaaca
    18421 tcgttcggaa aaggatgcac accacatcta cctcactgac ggcacttact tctgcgccac
    18481 taatgtggcg cgggtaaacc ttatccgaca ggtacaggag ccacgcagat gaccattctg
    18541 gactacatcg ctactcatcc ggggtgtagc ggcggagaga tcgccgcagc actgaatact
    18601 ccaaccacag ccattaatgc tgagttacgc caactttggc gcggcggctt agtcatcaga
    18661 acaaaccgca gcacaggtgg tcgcgctcgc aaaactggag gccaggcttc ttaccacgta
    18721 aacccgatgc cgttcgggtg tagcaatcca cttactcaca tgtttaacca gctactgaag
    18781 gaagccagaa catgagcacc atcaaccacc agaagctacg cgaactggca tttgccctgc
    18841 aacgaatggc aacacctcaa aaattactgg catttcgcgc aatgctctcg ccgtctgctg
    18901 cgctggcatt gctggatgag ctggagcacg ccagaaccac ggctcctgcc attcgcctga
    18961 cgctccatca tgaaatcgct gatttctgcg cgacattgga ggtgccaggc gaaccggaaa
    19021 cgccggaagc aatacagcaa gagctgctgc aacgtattga caaggttttt gatttttttc
    19081 tgaaccagta agaaaccaga gcatgcacac acaaaaaaac cgcttgccat gccgcaatca
    19141 gtcaggttac atttccgctg cacctcataa aacgggtgcc gggtttcgca gcctgctgac
    19201 tacacaagcg cacaaccgcg ccagcggttt ttttgtgcgt actgtattgc cacgtttttt
    19261 tcgcgtcaga attatggcgg ggcgtacggg gccgacttcg gtcgggccgg gttcttgtgt
    19321 agccggtact gcgaacctcg tacgtctcgc cacccacagt ttcgcagctc tggatggtga
    19381 gttttcacaa cttactacac aaggggccac accatggcaa accgcaaaca gcaccgcgct
    19441 atcgcggagc gtcgtcacat ccagactgaa atcaaccgca gactttcccg cgcatcacgc
    19501 gtcgcgcaaa tcatgcacat caatatgctg catgagcgca gccacgcact atcaaacatt
    19561 tattccgcct ctgttttcag ctatctggcg gatgatctgc acgagcttca acagctcatc
    19621 cagcagcaaa acaaactcca ttaattcctg ttccgggcct ttcctgcacc ttgcggcggg
    19681 aggccttcgc acatctgtaa caagaggatt gccgcaatga ttctcgccaa cgactttctt
    19741 gaatacctgc tcaatacaga acgtgatctt gccgctcgcg tgcgtgatcg ttatgacatg
    19801 tacctgaaat ccctgcctgt accgcagctc gctgacggaa agattgttat tgatggtcgc
    19861 tacatgattg acagccacga gggaaattac aggctttacc gcattgaagg tggcaccccg
    19921 tccgttattg gcatttacca gcgcccatcc tctgcaatcg tcgatgtgat tgccgacagc
    19981 atccgcatca cacatcgcca tgccgacaca gaagacaccg tgctggaaat tcagcggctg
    20041 gctacagtct gccgcgacac cctgaatggc atgacgaagt aaatcactat gacggcagag
    20101 tacctcaggg actggcaaca accgcgccac gcagtggggc gtgaaggaac ggggatcccc
    20161 gctcctgaat ccgcgctttc ctcctggctg gatgcctacc gggcagagaa cgagcgccgc
    20221 caggaaatgg ctgatgcggc gttctcagcc acgccgctgg gcaacctgat taataaaagc
    20281 ctggacgcac aggaaaaaca ggacaaaacc atcacactgg caggagacgc cagaaaacag
    20341 gcacgtggcg cggtggatga agccatggcc tcgctgcgcc tgctgccgtc ctatctgcgc
    20401 gatccgctta ttcgccacct ctccttcctg cgcaaaaaac aggaagccga tcgccggaaa
    20461 ggcaaaaaga gctggcaggc ggaacgctat gcacgcggaa ccctgcgcaa aatattcgaa
    20521 cgtctggata gcactgacgg acgctggctg acaccgggtt atcgctccct tgccggacgc
    20581 gaacgcctgg acgatttgct ttacctgccg cagctcaaca aacaccagat acagacgctg
    20641 gccaccatga cggcggcgat gttcagcagc accttcgaaa aactctgcga tggctttggc
    20701 gcgaccgatg gcgagctgac catggatgta accctgaagg cgtatcagat gctggcccgc
    20761 atggcgttac acctgcacgc catgcctcca cattatgacg cactgacaac agacaaagac
    20821 cggaggaacg aaccggacac ggagctgctg ccgggcgcaa tccttcgcct gacctgtgcg
    20881 gaatggtgga aacgcaaact gtggctgtta cgttgcgagt ggagagaaga acaactccgc
    20941 gccgcctgtc tggtttccag aaaaacatca ccctatctga gccaggacgc gttaagcgag
    21001 tttcgcgcac agcgcgagaa aacacgcgat ttcctgaaaa gtttcatgct ggaaaatgaa
    21061 gacgggttca cgattgatct cgagacagtg tattacgcgg gagtaagtaa cccggttcac
    21121 cgtaaggcag aaatgatggc caccatgaag ggactggaac ttctggccga agcccgtggc
    21181 gacaaagcgg tgtttctgac tgtcacctgc ccgtcaaaat accacgccac aacggagaac
    21241 ggtcatccga atcccaaatg gaacggggcc acaatgcgcg actccagcga ttacctggtt
    21301 aacacgtttt ttgcggcggt ccgcaaaaaa ctgaaccgcg acggcctgcg ctggtatggc
    21361 atccgcacgg tggagcctca tcatgacggc accgtgcact ggcatatgat ggtctttgca
    21421 catccggaag aaatcgacac cattgtgtcc catacccgcg atattgccat tcaggaagac
    21481 cgccacgagc tgggcaatga tattactccg cgctttaagg tggagtatgt cgacggctca
    21541 aaaggcacgc caaccagcta catcgccacc tacatcggaa agaacctgga cagccgcgcc
    21601 gtggatggca tcgacccgaa aacgggcaag ccacgcgtgg accacgaaac cggaaaatca
    21661 atggccgaga gcgtggagcg cgccatcggc tgggcgcgcc ttcaccgggt ccgtcagttc
    21721 cagttctttg gcatcccctc ccgtcaggtg tggcgtgaac tgcgccgcct tgccagccag
    21781 atggcacgca atccggaagg cccgcaacgg ctgaaagatg atgcaatgga tgcggtactc
    21841 gctgccgccg atgccgggtg ttttgccacc tacattgaaa aacagggtgg cgtgcttgtt
    21901 ccacgcaagg actacctgat tcgcaccgcc tacgacctcg cagatgagct gaacgattac
    21961 ggcgaacaga gcgtacagat ttacgggatc tggtcaccac tcatcgggga atcctcccgt
    22021 gtgtgcacgc atccggataa ctggaagctg gtaagacgta aaccggaagc ggaagacagc
    22081 gcccgcgaaa atggttttga ccttcagggc ggccctgccg ccccttggac tcgtggcaat
    22141 aactgtcccc gtgtacagga aacggacaac aacgggacag aacagccgga agaacggcca
    22201 gcaccgtggc cgcagctccc tgacggcgtt gaagtgaacg aatggatgcg ctcactgaaa
    22261 cgacacgaac gccgggcgct gatgcgttcg cttcgtgaca aacaggcaaa aaacagcagc
    22321 gatgaaatgc agagctggac acagagccgc aaacagcagc ggcctttgcc tgataaccac
    22381 gaattactcg ctaaagaatg gcgggagtct gctgaatctc tcggcctgca tatcggtgaa
    22441 caacagatgc agcacctgtt acggggcggc agtctgtacg ttgacggcag catcattgca
    22501 ccgcagggat ttgaaattgt acgcaaaccg gatacccgcc cggacagccg aatcacgcag
    22561 ctctggcagc gcctgagccg taatcatggc gtaagcagca cggagatccg ccataacccg
    22621 gtcgccagct atctggcaca gctgggggca tcagaccctg aagccgccgc acgcctggca
    22681 tccacacttc agcagggcca gaacaccatg aaaacacccg ttaccgtgct ttctgacatg
    22741 ctgcgcgcca tccgcgacgc agagcacgca cagagaatca gtgaaaccac tgaacgcgcc
    22801 agccgcaaag cagacctgct gcggggtggc ctgaccagtg gaaacaaaaa acagacagaa
    22861 acgggactta cgaatcccgt aaatgagcaa aaaacgcgca gcgatatatg aagcgcgcac
    22921 aaaacaggca aaaacgggat ttcagaatcc cgtaaacgat taattaatca acataaggaa
    22981 aagcgacatg aaaatttgta tcgacgacgg ctccaccaac atcaagctgg catggactga
    23041 gaatggcgaa cgccgcaacg ccatcagccc gaacagcttc aagtcggaat ggtctgcgcc
    23101 gttcggtggc atgcagcccg cgaactacat gcttgatggc gtgcgctatg gttttgatcc
    23161 ggttagcgat cgctttgtcc agacgaccga cacgcaatac cagtacagcg atgtgaatgt
    23221 aatcgccatt catcacgcgc tggtcaaatc aggcatcacg ccacaggagg tggatgtggt
    23281 tgtcaccctg ccactgagcg agtatttcga cacaaacgca cagccggaca tggccaacat
    23341 caaccgcaaa aaagcgaacg ttatgcgccc ggtggaatac cagaacggcg aagcattcac
    23401 tatccgtaac gtgcgggtta tgcctgaatc cattccggct ggctttaaag cactggctga
    23461 catgagtccg tttgaatccc tgctgattgt ggatttaggc ggaaccacgc tggatgtggc
    23521 aaaggttcaa gggcaactgg caggtatcag ccaggtgttt tgcgatccac acgtaggcgt
    23581 ttccctgatg gccgatgccg tactgtcggt gatggccact aacggtatgc gcaccagtca
    23641 ccacatcgcc aataccatta tcgaacatcg ccatgatgaa gcctggctgc gccagcacat
    23701 ccacaatgac gcgcattacg ccagcctgat ggcggttatt cgtgaaaagg aagaaacact
    23761 gaaacaacgc gtgatccgcg cgctggcggt tttttcgggt tacgggcggg tgatggttgt
    23821 cggtggaggg gcggagattg tggcacccgc tatccgcgaa gtctgcggag ttaatgcgac
    23881 tttcatcgcg gacggggtgc cacagtttgc tctggttaat gggctgtacg caatggacaa
    23941 ggagtaaacc aatgacgaca ccaaccagac ggataagttt ctatctgaag cccgccgccg
    24001 tcaagagtga acaggaggcg tgcaattacc tcgatagcct gccagcctcc gaacgcagcc
    24061 gcgcgcaacg cgcggccttt ctggctgggc tagcgctcat aaagcgcaac ccagcatttg
    24121 cctactggat ggccgaatgg ccagaggaca acctcccctt caataccgtt agcatcaagg
    24181 aaacatacca ggataatcag cctagcggag ttgaatgcaa tttttataaa ataaaaaaga
    24241 atattcagac cttattccca gaataatctg ataattgtat gtgaactaat aaaatacaga
    24301 ccaacacatc attctggcac ccttgctaac agggtgccaa tgtttcaaat catgatgaaa
    24361 ttgccgaagt ttttttatcg tgctgaagta ccgcaaatgc cattaaaacc tcaagagaat
    24421 agcggcgata atacttaaca aaaccataat aaaatactcg ggatgcagca at
//
LOCUS       DAZGCS010000073        24278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGCS010000073 DAZGCS010000000
VERSION     DAZGCS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.073"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..310
                     /locus_tag="ACHK01_003142"
     CDS             <1..310
                     /locus_tag="ACHK01_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098059.1"
                     /translation="GFYMGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILALFILS
                     LTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR
                     "
     gene            complement(357..1244)
                     /gene="rarD"
                     /locus_tag="ACHK01_003143"
     CDS             complement(357..1244)
                     /gene="rarD"
                     /locus_tag="ACHK01_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO8098060.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVV
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1296..1763)
                     /gene="yigI"
                     /locus_tag="ACHK01_003144"
     CDS             complement(1296..1763)
                     /gene="yigI"
                     /locus_tag="ACHK01_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO8098061.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1928..2797
                     /gene="pldA"
                     /locus_tag="ACHK01_003145"
     CDS             1928..2797
                     /gene="pldA"
                     /locus_tag="ACHK01_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO8098062.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2930..4759
                     /gene="recQ"
                     /locus_tag="ACHK01_003146"
     CDS             2930..4759
                     /gene="recQ"
                     /locus_tag="ACHK01_003146"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO8098063.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFSEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4823..5443
                     /gene="rhtC"
                     /locus_tag="ACHK01_003147"
     CDS             4823..5443
                     /gene="rhtC"
                     /locus_tag="ACHK01_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO8098064.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5505..6125)
                     /gene="rhtB"
                     /locus_tag="ACHK01_003148"
     CDS             complement(5505..6125)
                     /gene="rhtB"
                     /locus_tag="ACHK01_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO8098065.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6236..7258
                     /gene="pldB"
                     /locus_tag="ACHK01_003149"
     CDS             6236..7258
                     /gene="pldB"
                     /locus_tag="ACHK01_003149"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO8098066.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7266..8066
                     /gene="yigL"
                     /locus_tag="ACHK01_003150"
     CDS             7266..8066
                     /gene="yigL"
                     /locus_tag="ACHK01_003150"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO8098067.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8142..9041
                     /gene="bioP"
                     /locus_tag="ACHK01_003151"
     CDS             8142..9041
                     /gene="bioP"
                     /locus_tag="ACHK01_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO8098068.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8929..9882)
                     /gene="metR"
                     /locus_tag="ACHK01_003152"
     CDS             complement(8929..9882)
                     /gene="metR"
                     /locus_tag="ACHK01_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO8098069.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10005..12266
                     /gene="metE"
                     /locus_tag="ACHK01_003153"
     CDS             10005..12266
                     /gene="metE"
                     /locus_tag="ACHK01_003153"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO8098070.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12305..13120)
                     /gene="ysgA"
                     /locus_tag="ACHK01_003154"
     CDS             complement(12305..13120)
                     /gene="ysgA"
                     /locus_tag="ACHK01_003154"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO8098071.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13382..14143
                     /gene="udp"
                     /locus_tag="ACHK01_003155"
     CDS             13382..14143
                     /gene="udp"
                     /locus_tag="ACHK01_003155"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO8098072.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14284..15711
                     /gene="rmuC"
                     /locus_tag="ACHK01_003156"
     CDS             14284..15711
                     /gene="rmuC"
                     /locus_tag="ACHK01_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO8098073.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15806..16561
                     /gene="ubiE"
                     /locus_tag="ACHK01_003157"
     CDS             15806..16561
                     /gene="ubiE"
                     /locus_tag="ACHK01_003157"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO8098074.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16575..17180
                     /gene="ubiJ"
                     /locus_tag="ACHK01_003158"
     CDS             16575..17180
                     /gene="ubiJ"
                     /locus_tag="ACHK01_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO8098075.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17177..18817
                     /gene="ubiB"
                     /locus_tag="ACHK01_003159"
     CDS             17177..18817
                     /gene="ubiB"
                     /locus_tag="ACHK01_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO8098076.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18896..19165
                     /gene="tatA"
                     /locus_tag="ACHK01_003160"
     CDS             18896..19165
                     /gene="tatA"
                     /locus_tag="ACHK01_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO8098077.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19169..19684
                     /gene="tatB"
                     /locus_tag="ACHK01_003161"
     CDS             19169..19684
                     /gene="tatB"
                     /locus_tag="ACHK01_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO8098078.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19687..20463
                     /gene="tatC"
                     /locus_tag="ACHK01_003162"
     CDS             19687..20463
                     /gene="tatC"
                     /locus_tag="ACHK01_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO8098079.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20505..21287
                     /gene="tatD"
                     /locus_tag="ACHK01_003163"
     CDS             20505..21287
                     /gene="tatD"
                     /locus_tag="ACHK01_003163"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO8098080.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21284..21772)
                     /gene="rfaH"
                     /locus_tag="ACHK01_003164"
     CDS             complement(21284..21772)
                     /gene="rfaH"
                     /locus_tag="ACHK01_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO8098081.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21939..23432
                     /gene="ubiD"
                     /locus_tag="ACHK01_003165"
     CDS             21939..23432
                     /gene="ubiD"
                     /locus_tag="ACHK01_003165"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO8098082.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23478..24179
                     /gene="fre"
                     /locus_tag="ACHK01_003166"
     CDS             23478..24179
                     /gene="fre"
                     /locus_tag="ACHK01_003166"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO8098083.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
BASE COUNT         5714 a         6047 c         6651 g         5866 t
ORIGIN      
        1 tggattctat atgggaaatg gtaacttttg gcagttgttc ttgggtaaag aaccttacag
       61 caaaaaatct gattttgtta aggcatggtt attgtggtgg gctattacca tccttgctct
      121 atttatttta agcctgacta cgacattaaa tttaagaagt ataggtatag ggttaagttt
      181 tgggttttgt caaatccttg gtagatatac gatgtctcta ggatggagta agtactgggg
      241 agttttgggg gtatttcctg taacaaatat tgttcttttt cttatcttaa tttttttcaa
      301 aaaaagataa ataagtcagt gggtaggctc tttaaacggc ctacccatgt taattcttaa
      361 ctttttctac gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata
      421 aaggcgaaag tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc
      481 aggaacatca gcgtcgggcc aatgtactgg aaaaagccta acgttgagag acgcaagcgc
      541 gtggcagcgg cggtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc
      601 agtaaattca gcgacatcgg gttttgcccc atatggctgg ttgagctgtc ggcaatagca
      661 aacaggtaaa ttgccgccac gggcagcagc cacatggttt cgattaacat gccggtttgc
      721 gcttcaacgg caatcttctt gcgtaccaga ccgtagaagg caaaactaaa tgccagtccc
      781 agcgcgataa taggtagcga accaaaagtc cacagctgga ctaacacgcc acatatcgcc
      841 agaatcaccg ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc
      901 acaatgttca ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg
      961 ttcaccgccc agataaacag tagccagttg ccaccaatca gcacggcaga gactgccagc
     1021 ataaaaattt tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg
     1081 ctcatcagca ccaccataaa gaaaaacgac cagaccacgc gatgcgtcag gatttcatcg
     1141 gcaggcacgt agtaaatcaa cttgaagtac gctggcgcta taccccaaat aaaataagcg
     1201 gcaagagcga gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt
     1261 taattggtaa cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg
     1321 gcactggcaa tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta
     1381 ttgcctgcac gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga
     1441 taatcaacgc gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca
     1501 ctgatggttt cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc
     1561 agcgccgacg caatgacccc gccgtgcaaa atgctttgcg cccagttgcc caccatcatt
     1621 ggctgatttt taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc
     1681 aatgcgcggt taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt
     1741 tcagcggtca gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat
     1801 tttatgcttc tttgttgttg gtttctactt taggaaggga taactaacgg ctatggagtt
     1861 aagtatgtaa aatagcccgc agaaaaatat tcaccttatc aataattcgt tacggagaac
     1921 acgaccgatg cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt
     1981 atatgcacaa gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat
     2041 tatcgccaat atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa
     2101 ctacctcatt tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg
     2161 ggcggaaaat gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg
     2221 gcgtgggatt ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg
     2281 gcaactgtcc aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt
     2341 gttcctcggt tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat
     2401 ggggtataac cacgactcta acgggcgttc cgacccgacc tcccgcagct ggaaccgcct
     2461 ttatactcgc ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt
     2521 ggtgggtaat actgacgata acccggatat caccaaatat atgggttact accagcttaa
     2581 aatcggctat cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac
     2641 cggctacggc ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta
     2701 tactcaggtt tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg
     2761 tgtcggtgtg ggggttatgc taaacgattt gttttgatga acggttgagt ggttggcaaa
     2821 tctggaatcc agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg
     2881 caggcgctga aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc
     2941 ggaagtgttg aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca
     3001 acagtttcgc cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct
     3061 ggtcgtcatg cccaccggtg gcggaaaatc cctttgctat caaattcctg ccttattgct
     3121 aaacggcctt accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca
     3181 actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca
     3241 acttgaagtg atgacaggct gccgcaccgg gcaaattcgt ctgctttata tcgccccgga
     3301 acgcctgatg ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc
     3361 cgttgatgaa gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc
     3421 gctcggtcag ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc
     3481 cgacgacacc acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca
     3541 aatcagcagt tttgaccgtc cgaatattcg ctacatgctg atggagaagt tcaaaccgct
     3601 cgatcagttg atgcgctacg tgcaggaaca gcgcggtaag tccggcatta tctactgcaa
     3661 cagccgggcg aaagtagaag acaccgctgc gcgcctgcaa agcaagggta ttagcgcggc
     3721 ggcctatcat gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg
     3781 cgatgacctg caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa
     3841 cgttcgcttc gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac
     3901 cggtcgcgcc gggcgtgatg gcctgcccgc ggaagcgatg ctgttttacg atccggccga
     3961 tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga
     4021 acgtcataaa ctcaatgcga tgggcgcttt tgccgaagcg caaacttgcc gtcgtctggt
     4081 actgctgaac tattttagcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct
     4141 cgatccgccg aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg
     4201 tcgtgtgaat cagcgttttg gtatgggcta tgtggtggaa gtgattcgcg gcgctaataa
     4261 ccagcgtatc cgcgactatg gtcatgacaa actaaaagtc tacggtatgg gccgtgataa
     4321 aagtcatgaa cattgggtga gcgtgatccg ccagttgatt cacctcggcc tggtgacgca
     4381 aaatattgcc cagcattctg ccctccaact gacagaggcc gcgcgtccgg tgctgcgcgg
     4441 cgaatcctct ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca
     4501 gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa
     4561 atcgattgcc gatgaaagca atgtcccgcc gtacgtggtg tttaacgacg caacgttgat
     4621 tgagatggct gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg
     4681 gatgcgcaag ctggaacgct ttggtaaacc gtttatggcg cttatccgcg cgcatgttga
     4741 cggcgacgac gaagagtagt cagcggcata aaaaagtgcc aggatgaaga ctccgtaaac
     4801 gtttccccca cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcatat
     4861 tgtggcgctt atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg
     4921 ttcccgtaaa gaagcgatga tgggcgtgct gggcattacc tgcggtgtaa tggtttgggc
     4981 tgggattgcg ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct
     5041 gattatggtt ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc
     5101 actgaaaaaa gaggcggttt ctgcacctgc gccacaggtc gagctggcga aaagtgggcg
     5161 cagtttcctg aaaggtttac tgaccaatct cgctaatccg aaagcgatta tctactttgg
     5221 ctcggtgttc tcattgtttg tcggtgataa cgttggcact accgcgcgct ggggcatttt
     5281 tgcgctgatc attgtcgaaa cgctggcgtg gtttaccgtc gttgccagcc tgtttgccct
     5341 gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc
     5401 gttatttgcc ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca
     5461 gagtaagtcg gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc
     5521 gatgctaaca gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc
     5581 atctgctttg gtcctttaat ccatagagca atccgttgag caagggtggc gtaaccgatc
     5641 atcacaataa tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc
     5701 ggctgttgcg gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga
     5761 ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc
     5821 agcgatttaa ggtcaattgc accagcggcg cgccactgct ggattcccag ccaaatcaag
     5881 taagccgcgc ctgcccactt caacacttca aacgcaatca ctgagcggga aaatagcgtc
     5941 cccaacccca cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata
     6001 gacgccaccg cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca
     6061 gagcctggcg acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag
     6121 gtcatgatga actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt
     6181 gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt
     6241 tcagcagcaa aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc
     6301 gctgactgat ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc
     6361 ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg
     6421 gcgtattgag agctacgtaa aatatgcgga actggcctac gatttgttcc atttggggtt
     6481 tgatgtctta atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc
     6541 gcatctcggg catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca
     6601 acaggaggtc cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg
     6661 tgcgatctcc acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac
     6721 tgcgccaatg tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa
     6781 ctgggccgaa gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg
     6841 cgcgttgccg tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt
     6901 acgcttctat gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg
     6961 cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac
     7021 gcttctcttg caggctgaag aggaacgcgt ggtggataac cgcatgcatg accgtttttg
     7081 tgaactccgc accgccgcgg gccatcctgt cgaaggagga cggccgttgg taattaaagg
     7141 tgcttaccat gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat
     7201 cgttgatttt ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat
     7261 ttcttatgta ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata
     7321 cgttatcccc ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg
     7381 tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta
     7441 agtcttacat gattacctcc aatggtgcgc gcgttcacga tctggatggt aatctgattt
     7501 ttgctcataa cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc
     7561 cggacatcat tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg
     7621 aagagatgcg cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag cctggattac
     7681 tggagccgga aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa caactgctgc
     7741 cgctggagca ggcgattaac gctcgttggg gagatcgcgt caacgtcagt ttctctacct
     7801 taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg
     7861 cgaagaaact gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg
     7921 ccgaaatgct gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga
     7981 aagaccttca tcccgagctg gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt
     8041 atctgcgtaa actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac
     8101 ttcgctacaa tggatactcg ttaatcaaag agttttccat tgtggcgcta cttatcatca
     8161 ccacgattct gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg
     8221 atagctattt tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc
     8281 tgcgtacccg tggcaatagc ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc
     8341 agcttggcgt gatgtatatg ctgagtttcc gcgcttatct ctatctgacg gtttccgaac
     8401 tgctgctgtt caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta
     8461 agcgccgtct gcgctgggga tatgctttta gtgccttgtt ggcggtgatt ggtgccggga
     8521 ttattcgcta tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct
     8581 ccaatatcac ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga
     8641 tgccgcagca taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg
     8701 catggttctt gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc
     8761 tggtgtttct tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga
     8821 cgcaggtgga cgccggaacg ctgggaatta tgaataatat gcacgttccg gcagggctgc
     8881 tggtaaacct ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc
     8941 tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa
     9001 cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg
     9061 acgctgctcg ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt
     9121 tttggtcacc accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc
     9181 aatacccatc cgcgcggcaa ccatctgaat caacaataag gtgttatcga cgcttttcag
     9241 tgacgggctg acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg
     9301 caccggataa atcaacagcg tctcgctggc gagatcttcc ggtgtaattc gcgttttcgc
     9361 cgccagtgga tggtcaggtg ctaacaccag acgcacttca tagtcgaaca tcggcgaata
     9421 atgcaggcca ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg
     9481 caacgcgggc tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca
     9541 gttcttatgg aaattttcta acgcgggcgt cagccactga atacagctat ggcactcaat
     9601 ggcaatgcgc agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctaatttg
     9661 cggcagcacc tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg
     9721 ctggctctta cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg
     9781 agacagggcg gattgcgtct gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt
     9841 ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg
     9901 catgaataat ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc
     9961 tggacggcta aatactaaaa attccataag gggcatatca tataatgaca attcttaatc
    10021 acaccctcgg tttccctcgc gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt
    10081 attgggcggg gaactccacg cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc
    10141 gtcactggga tcaacaaaag caagcgggta tcgacctgct gccggtgggc gattttgcct
    10201 ggtacgatca tgttctgacc accagtctgc tgctgggtaa tgttccgcca cgtcatcaga
    10261 acaaagacgg ttcggtagat atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga
    10321 ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt taacaccaac tatcactaca
    10381 tggtgccgga attcgttaaa ggccaacagt tcaaactgac ctggacgcag ctgctggagg
    10441 aagtggacga ggcgctggcg ctgggccaca acgttaagcc tgtgctgctg gggccggtta
    10501 cttacctgtg gctggggaaa gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg
    10561 acattctgcc ggtttatcag caagtgctgg cagaactggc gaaacgcggc atcgagtggg
    10621 tacagattga tgaacccgcg ctggtactgg aactaccaca ggcgtggctg gacgcataca
    10681 aacccgctta cgacgcgctc cagggacagg tgaaactgct gctgaccacc tattttgaag
    10741 gcgtaacgcc aaatctcgac acgattactg cgctgcctgt tcagggtctg catgttgacc
    10801 tcgtacatgg taaagatgac gttgctgaac tgcacaagcg cctgccttct gactggttgc
    10861 tgtctgcggg tctgatcaat ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg
    10921 cgcaaattaa ggacattgtc ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc
    10981 tgcacagccc catcgacctg agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt
    11041 ttgccttcgc cctacaaaaa tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg
    11101 gtgacacggc agctctggca gagtggagcg ccccgattca ggcacgtcgt cactctaccc
    11161 gcgtacataa tccggcggta gaaaagcgtc tggcggcgat caccgcccag gacagccagc
    11221 gtgcgaatgt ctatgaagtg cgtgccgaag cccagcgtgc gcgttttaaa ctgccagcgt
    11281 ggccgaccac cacgattggt tctttcccgc aaaccacgga aattcgtacc ctgcgtctgg
    11341 atttcaaaaa gggcaatctc gacgccaata actaccgcac gggcattgcg gaacatatca
    11401 ggcaggccat tgttgaacag gaacgtttgg gactggatgt gctggtacat ggcgaggccg
    11461 agcgtaatga catggtggaa tactttggcg agcacctcga cgggtttgtc tttacgcaaa
    11521 acggttgggt acagagctac ggttcccgct gcgtgaagcc accgattgtt attggtgacg
    11581 ttagtcgccc ggcaccgatt accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca
    11641 aaccggtgaa agggatgctg acggggccgg tgaccatact ctgctggtcg ttcccgcgtg
    11701 aagatgtcag ccgtgaaacc atcgccaaac agattgcgct ggcgctgcgt gatgaggtgg
    11761 ccgatctgga agcggcaggg attggtatca tccagattga cgaaccggcg ctgcgcgaag
    11821 gtttaccact gcgtcgtagc gactgggacg cctatctcca gtggggcgta gaggccttcc
    11881 gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca cactcacatg tgttattgcg
    11941 agttcaacga catcatggat tccattgcgg cgctggatgc agacgtcatc accatcgaaa
    12001 cctcgcgttc cgacatggag ttgctggagt cgtttgaaga gtttgattat ccgaatgaaa
    12061 tcggtccagg cgtctatgac attcactcgc caaacgtacc gagcgtggaa tggattgaag
    12121 ccctgctgaa gaaagcggca aaacgcattc cggcagagcg cctgtgggtc aacccagact
    12181 gtggcctgaa aacgcgcggc tggccagaaa cccgcgcggc actggcgaac atggtgcagg
    12241 cggcgcagaa tttgcgtctg gggtaaatcc aaaccgggtg gtaataccac ccggtctttt
    12301 ctcattacag cgacttcttc ccaccatact gcttaaacca ttccagcata cgctgccagc
    12361 catctttcgc agattcggca tgatagctcg ggcgataatc agcgttgaat gcatgcccgg
    12421 catccgggta gacgataatc tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg
    12481 tttcaacgct ctcctgcgga atgctgttat cctgaccgcc atataagccg agaaccggcg
    12541 cgttaagatc ggttgcgata tcaacaggtt gtttcggtga attcagcgac ttatcgcccg
    12601 tcagtttgcc gtaccacgcc actgcggctt ttagctgtgg attatgcgcg gcatacagcc
    12661 aggtgatacg tccaccccag cagaatccgg tgatcattaa acgatgaaca tcgccgccgt
    12721 tgcgggaagc ccaactggcg acatgatcga gatcggccag cacctgcgag tcaggcactt
    12781 ttgctaccag accgctaagc aacgtgggga tatcggcaaa atcattcgga tcgccttcgc
    12841 ggaagtaaag ttcaggtgcg atagccagat acccctccag cgccagacgg cgacaaatgt
    12901 cgcggatatg ttcatgcacg ccaaaaattt cctgcactac aatgaccact ggcagtgggc
    12961 catcgctttg ctttggtctg gcatggtaag caggcatgtt atccccttgt gaagggatag
    13021 aggtgaagcc cgccacaatc gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg
    13081 atgcagcagg tgcaaatcca gattgttgtg ttgttgccat ggtattctcc gtacccttat
    13141 aaaaatgttg cgcaatgtta actatagtca gcatgcaaca aatcacattg cctgaatcgg
    13201 ctcatctttt atgcagtcct gcagaatgaa gagtgattta tgtgatttga atcacttttg
    13261 gtgggtaaat ttatgcaacg catttgcgtc atggtgatga gtatcacgaa aaaatgttaa
    13321 acccttcggt aaagtgtctt tttgcttctt ctgactaaac cgattcacag aggagttgta
    13381 tatgtccaag tctgatgttt ttcatctcgg cctcactaaa aacgatttac aaggggctac
    13441 gcttgccatc gtccctggcg acccggatcg tgtggaaaag atcgccgcgc tgatggataa
    13501 gccggttaag ctggcatctc accgcgaatt cactacctgg cgtgcagagc tggatggtaa
    13561 acctgttatc gtctgctcta ccggtatcgg cggcccgtct acctctattg ctgttgaaga
    13621 gctggcacag ctgggcattc gcaccttcct gcgtatcggt acaacgggcg ctattcagcc
    13681 gcatattaat gtgggtgatg tcctggttac cacggcgtct gtccgtctgg atggcgcgag
    13741 cctgcacttc gcaccgctgg aattcccggc tgtcgctgat ttcgaatgta cgactgcgct
    13801 ggttgaagct gcgaaatcca ttggcgcgac aactcacgtt ggcgtgacag cttcttctga
    13861 taccttctac ccaggccagg aacgttacga tacttactct ggtcgcgtag ttcgtcactt
    13921 taaaggttct atggaagagt ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc
    13981 aaccctgctg accatgtgtg caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat
    14041 cgttaaccgc acccagcaag agatcccgaa tgctgagacg atgaaacaaa ctgaaagcca
    14101 agcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg taattctctt ctcctgtctg
    14161 aaggccgacg cgttcggcct tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc
    14221 aactggacgt ttgtacagca caattctatt ttgtgcgggt aagttgttgc gtcaggaggc
    14281 gttgtggatt tctcaatcat ggtttacgca gttattgcgt tggtgggtgt ggcaattggc
    14341 tggctgtttg ccagttatca acatgcgcag caaaaagccg agcaattagc tgaacgtgaa
    14401 gagatggtcg cggagttaag cgcggcaaaa caacaaatta cccaaagcga gcactggcgt
    14461 gcagagtgcg agttactcaa taacgaagtg cgcagcctgc aaagtattaa cacctctctg
    14521 gaggccgatc tgcgtgaagt aaccacgcgg atggaagccg cacagcaaca tgctgacgat
    14581 aaaattcgcc agatgattaa cagcgagcag cgcctcagtg agcagtttga aaacctcgcc
    14641 aaccgtattt ttgagcacag caatcgccgg gttgatgagc aaaaccgtca gagtctgaac
    14701 agcctgttgt cgccgctacg tgaacaactg gacggtttcc gccgtcaggt tcaggacagc
    14761 ttcggtaaag aagcacaaga acgccatacc ctgacccacg aaattcgcaa tctccagcaa
    14821 ctcaacgcgc aaatggccca ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat
    14881 aaaacccagg gcaactgggg cgaggtagta ttgacgcggg tgctggaggc ttccggtctg
    14941 cgtgaagggt atgaatatga aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg
    15001 cagccggatg tcatcgtgcg gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg
    15061 acgctggtcg cctatgaacg ctattttaac gccgaagacg actacacccg cgaaagcgcg
    15121 ctacaggaac atatcgcgtc ggtgcgtaac catatccgtt tgctgggacg caaagattat
    15181 caacagctgc cggggctgcg aactctggat tacgtgctga tgtttattcc cgttgaaccc
    15241 gcttttttac tggcgcttga ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat
    15301 atcatgctgg ttagcccgac tacgctgctg gtggcgttgc gtactattgc caacctgtgg
    15361 cgttatgagc atcaaagccg caacgcccag caaatcgccg atcgtgccag caagctgtac
    15421 gacaagatgc gtttgttcat cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg
    15481 caggataact accgccaggc aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg
    15541 caggcagaag cgtttcgcgg tttaggagta gaaattaaac gcgagattaa tccggatttg
    15601 gctgaacagg cggtgagcca ggatgaagag tatcgacttc ggtcggttcc ggagcagccg
    15661 aatgatgaag cttatcaacg cgatgatgaa tataatcagc agtcgcgcta gcccattagg
    15721 ggtagttaag ccgggtagaa atctagggca tcgacgccca atctgttaca cttctggaac
    15781 aattttttga tgagcaggca ttgagatggt ggataagtca caagaaacga cgcactttgg
    15841 ttttcagacc gtcgcgaagg aacaaaaagc ggatatggtc gcccacgttt tccattccgt
    15901 ggcatcaaaa tacgatgtca tgaatgattt gatgtcattt ggtattcatc gtttgtggaa
    15961 gcgattcacg attgattgca gcggcgtacg ccgtgggcag accgtgctgg atctggctgg
    16021 tggcaccggc gacctgacag cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt
    16081 ccttgctgat atcaatgaat ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg
    16141 tgtgattggc aacgttgagt atgttcaggc gaacgctgag gcgctgccgt tcccggataa
    16201 cacctttgat tgcatcacca tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc
    16261 actgcgttca atgtatcgcg tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc
    16321 gaagccaatt atcgagccgc tgagcaaagc ctatgatgca tactccttcc atgtgctgcc
    16381 gcgtattggc tcactggtcg cgaacgacgc cgacagctac cgttatctgg cagaatccat
    16441 ccgtatgcat cccgatcagg ataccctgaa agccatgatg caggatgccg gattcgaaag
    16501 tgtcgactac tacaatctga cggcgggggt tgtggcgctg catcgtggtt ataagttctg
    16561 acaggagacc ggaaatgcct tttaaacctt tagtgacggc aggaattgaa agtctgctca
    16621 acaccttcct gtatcgctca cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag
    16681 tattgcgcgt ggaggtaaaa ggcttttcga cgtcattgat tctggtgttc agcgaacgcc
    16741 aggttgatgt actgggcgaa tgggcaggcg atgctgactg caccgttatc gcctacgcca
    16801 gtgtgttgcc gaaacttcgc gatcgccagc agcttaccgc actgattcgc agtggtgagc
    16861 tggaagtgca gggcgatatt caggtggtgc aaaacttcgt tgcgctggca gatctggcag
    16921 agttcgaccc tgcggaactg ctggcccctt ataccggtga tatcgccgct gaaggaatca
    16981 gcaaagccat gcgcggaggc gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt
    17041 atgtggcgga agccattact gaagagtggc gtatggcacc cggtccgctt gaagtggcct
    17101 ggtttgcgga agagacggct gccgtcgagc gtgctgttga tgccctgacc aaacggctgg
    17161 aaaaactgga ggctaaatga cgccaggtga agtacggcgc ctatatttca tcattcgcac
    17221 ttttttaagc tacggacttg atgaactgat ccccaaaatg cgtatcaccc tgccgctacg
    17281 gctatggcga tactcattat tctggatgcc aaatcggcat aaagacaaac ttttaggtga
    17341 gcgactacga ctggccctgc aagaactggg gccggtttgg atcaagttcg ggcaaatgtt
    17401 atcaacccgc cgcgatcttt ttccaccgca tattgccgat cagctggcgt tattgcagga
    17461 caaagttgct ccgtttgatg gcaagctggc gaagcagcag attgaagctg caatgggcgg
    17521 cttgccggta gaagcgtggt ttgacgattt tgaaatcaag ccgctggctt ctgcttctat
    17581 cgcccaggtt cataccgcgc gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat
    17641 ccgcccggat attttgccgg ttattaaagc ggatctgaaa cttatctacc gtctggctcg
    17701 ctgggtgccg cgtttgctgc cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga
    17761 gtacgaaaag acattgattg atgaactgaa tttgctgcgg gaatctgcca acgccattca
    17821 gcttcggcgc aattttgaag acagcccgat gctctacatc ccggaagttt accctgacta
    17881 ttgtagtgaa gggatgatgg tgatggagcg catttacggc attccggtgt ctgatgttgc
    17941 ggcgctggag aaaaacggca ccaacatgaa attgctggcg gaacgcggcg tgcaggtgtt
    18001 cttcactcag gtctttcgcg acagcttttt ccatgccgat atgcaccctg gcaacatctt
    18061 cgtaagctat gaacacccgg aaaacccgaa atatatcggc attgattgcg ggattgttgg
    18121 ctcgctaaac aaagaagata aacgctatct ggcagaaaac tttatcgcct tctttaatcg
    18181 cgactatcgc aaagtggcag agctacacgt cgattctggc tgggtgccac cagataccaa
    18241 cgttgaagag ttcgaatttg ccattcgtac ggtctgtgaa cctatctttg agaaaccgct
    18301 ggccgaaatt tcgtttggac atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa
    18361 tatggaagtg cagccgcaac tggtgttact ccagaaaacc ctgctctacg tcgaaggggt
    18421 aggacgccag ctttatccgc aactcgattt atggaaaacg gcgaagcctt tcctggagtc
    18481 gtggattaaa gatcaggtcg gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc
    18541 gttctgggtc gaaaaaatgc cagaactgcc tgaactggtt tacgacagtt tgcgccaggg
    18601 caagtattta cagcacagtg ttgataagat tgcccgcgag cttcagtcaa atcatgtacg
    18661 tcagggacaa tcgcgttatt ttctcggaat tggcgctacg ttagtattaa gtggcacatt
    18721 cttgttggtc agccgacctg agtgggggct gatgcccggc tggttaatgg caggtggtct
    18781 gatcgcctgg tttgtcggtt ggcgcaaaac acgctgattt tttcatcgct caaggcgggc
    18841 cgtgtaacgt ataatgcggc tttgtttaat catcatctac cacagaggaa catgtatggg
    18901 tggtatcagt atttggcagt tattgattat tgccgtcatc gttgtactgc tttttggcac
    18961 caaaaagctc ggctccatcg gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc
    19021 aatgagcgat gatgaaccaa agcaggataa aaccagtcag gatgctgatt ttactgcgaa
    19081 aactatcgcc gataagcagg cggatacgaa tcaggaacag gctaaaacag aagacgcgaa
    19141 gcgccacgat aaagagcagg tgtaatccgt gtttgatatc ggttttagcg aactgctatt
    19201 ggtgttcatc atcggcctcg tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac
    19261 ggtagcgggc tggattcgcg cgttgcgttc actggcgaca acggtgcaga acgaactgac
    19321 ccaggagtta aaactccagg agtttcagga cagtctgaaa aaggttgaaa aggcgagcct
    19381 cactaacctg acgcccgaac tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc
    19441 gatgaagcgt tcctacgttg caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat
    19501 ccataacccg gtggtgaaag ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc
    19561 acaaacgcag gccagttcgc cggaacagaa gccagaaacc acgccagagc cggtggtaaa
    19621 acctgctgcg gacgctgaac cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc
    19681 gtaaacatgt ctgtagaaga tactcaaccg cttatcacgc atctaattga gctgcgtaag
    19741 cgtctgctga actgcattat cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc
    19801 aatgacatct atcacctggt atccgcgcca ttgatcaagc agttgccgca aggttcaacg
    19861 atgatcgcca ccgacgtggc ctcgccgttc tttacgccga tcaagctgac ctttatggtg
    19921 tcgctgattc tgtcagcgcc ggtgattctc tatcaggtgt gggcatttat cgccccagcg
    19981 ctgtataagc atgaacgtcg cctggtggtg ccgctgctgg tttccagctc tctgctgttt
    20041 tatatcggca tggcgttcgc ctactttgtg gtctttccgc tggcatttgg cttccttgcc
    20101 aataccgcgc cggaaggggt acaggtatcc accgacatcg ccagctattt aagcttcgtt
    20161 atggcgttgt ttatggcgtt tggtgtctcc tttgaagtgc ctgtggcaat tgtgctgctg
    20221 tgctggatgg ggattacctc gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt
    20281 ggtgcattcg ttgtcgggat gctgctgaca ccgccggatg tcttctcgca aacgctgttg
    20341 gcgatcccga tgtactgtct gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt
    20401 aaagggcgaa atcgggaaga ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa
    20461 taaattcaac cgcccgtcag ggcggttgtc atatggagta caggatgttt gatatcggcg
    20521 ttaatttgac cagttcgcaa tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg
    20581 acgcgggagt taatgggcta ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc
    20641 aaaagctggc gcgtcagtat tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca
    20701 gcagccagtg gcaagctgcg actgaagaag cgattattga gctggccgcg cagccagaag
    20761 tggtggcgat tggtgaatgt ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc
    20821 aggaacgcgc ttttgttgcc cagctacgca ttgccgcaga tttaaacatg ccggtattta
    20881 tgcactgtcg cgatgcccac gagcggttta tgacattgct ggagccgtgg ctggataaac
    20941 tgcctggtgc ggttcttcat tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg
    21001 cgcgtggaat ttatatcggc attaccggtt gggtttgcga tgaacgacgc gggctggagc
    21061 tgcgggaatt gttgccgttg attccggcgg agaaattgct gatcgaaact gatgcgccgt
    21121 atctgctccc gcgcgatctc acgccaaagc catcatcccg gcgcaacgag ccagcccatc
    21181 tgccccatat tttgcaacgt attgcgcact ggcgtggaga agatgccgca tggctggctg
    21241 ccaccacgga tgccaatgtc aaaacactgt ttgggattgc gttttagagt ttgcggaact
    21301 cggtgttctt cacactgtgc ttaatctctt tattaataag attaagcaat agcatggagc
    21361 gagcctcacc atcgggttcg gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa
    21421 taatcacctt atcacccgga taaggggttg ccggatcgac aatgtctttc ggtttatata
    21481 ccgatagctg atgaatcacc gccgatggga ctatcgctgg cgacgcgcca aagcgcacga
    21541 agtggctgac accgcgggtc gcgttgatag tcgtggtatg aatcacttct ggatcaaatt
    21601 cgacaaacag gtagttggga aacaatggct cactgactgc agtacgtttt ccacgcacga
    21661 ttttttccag ggtgatcatc ggtgccaggc aattcacagc ctgtctttcg aggtgttcct
    21721 gggcacgttg aagttgcccg cgcttgcagt acagtaaata ccaggattgc ataatgactc
    21781 ttatccgttt aatcggggcg caaggatagc aaaagcttta cgctaagtta attatattcc
    21841 ccggtttgcg ttataccgtc agagttcacg ctaatttaac aaatttacag catcgcaaag
    21901 atgaacgccg tataatgggc gtagattaag aggctacaat ggacgccatg aaatataacg
    21961 atttacgcga cttcctgaca ctgcttgaac agcagggtga gctaaaacgt atcacgctcc
    22021 cggtggatcc gcatctggaa atcactgaaa ttgctgaccg cactttgcgt gccggtgggc
    22081 ctgcgctgtt gttcgaaaac cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg
    22141 gtacgccaaa gcgcgtggca atgggcatgg ggcaggaaga tgtttcggcg ctacgtgaag
    22201 ttggtaaatt attggcgttt ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt
    22261 ttgataaact gccgcagttt aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg
    22321 cgccctgcca acaaaaaatc gtctctggcg atgacgtcga tctcaatcgc attcccatta
    22381 tgacctgctg gccggaagat gccgcgccgc tgattacctg ggggctgaca gtgacgcgcg
    22441 gcccgcataa agagcggcag aatctgggca tttatcgcca gcagctgatt ggtaaaaaca
    22501 aactgattat gcgctggctg tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg
    22561 cggcgcatcc gggcgaacgt ttcccggttt ctgtggcgct gggtgccgat cccgccacga
    22621 ttctcggtgc agtcaccccc gttccggata cactttcaga gtatgcgttt gccggattgc
    22681 tgcgcggtac caagaccgaa gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca
    22741 gtgcggagat tgtgctggaa gggtatatcg aacaaggtga aacggcgccg gaagggccgt
    22801 atggcgacca caccggttac tataacgaag tcgatagttt ccctgtcttt actgtgacgc
    22861 atattaccca gcgtgaagat gcgatttacc attccaccta taccgggcgt ccgcctgatg
    22921 aacctgcggt actgggcgtg gcgttgaacg aagtgtttgt gccgattctg caaaaacagt
    22981 tcccggaaat tgtcgatttt tacctgccac cggaaggctg ttcttatcgt ctggcggtag
    23041 tgacgatcaa aaaacagtac gccggacacg cgaagcgcgt catgatgggc gtctggtcgt
    23101 tcttacgcca gtttatgtac actaaatttg tgatcgtttg cgatgatgac gtcaacgcac
    23161 gcgactggaa cgatgtgatt tgggcgatta ccacccgtat ggacccggcg cgggatactg
    23221 ttctggtaga aaatacgcct attgattatc tggattttgc ctcgcctgtc tccgggctgg
    23281 gttcaaaaat ggggctggat gccacgaata aatggccggg ggaaacccag cgtgaatggg
    23341 gacgtcccat caaaaaagat ccagatgttg tcgcgcatat tgacgccatc tgggatgaac
    23401 tggctatttt taacaacggt aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga
    23461 tccgacagag aaagcgcatg acaaccttaa gctgtaaagt gacctcggta gaagctatca
    23521 cggataccgt atatcgtgtc cgcatcgtgc cagatgcggc cttttctttt cgcgctggtc
    23581 agtatttgat ggtagtgatg gatgagcgcg acaaacgtcc gttctcaatg gcttcgacgc
    23641 cggatgaaaa agggtttatc gagctgcata ttggcgcttc tgaaatcaac ctttacgcga
    23701 aagcagtcat ggaccgcatc ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag
    23761 aagcgtggct gcgcgatgat gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt
    23821 tctcttatgc ccgctcgatt ttgctgacag cgttggcgcg taacccaaac cgtgatatca
    23881 ccatttactg gggcgggcgt gaagagcagc atctgtatga tctctgcgag cttgaggcgc
    23941 tttcgttgaa gcatcctggt ctgcaagtgg tgccggtggt tgaacaaccg gaagcgggct
    24001 ggcgtgggcg tactggcacc gtgttaacgg cggtattgca ggatcacggt acgctggcag
    24061 agcatgatat ctatattgcc ggacgttttg agatggcgaa aattgcccgc gatctgtttt
    24121 gcagtgagcg taatgcgcgg gaagatcgcc tgtttggcga tgcgtttgca tttatctgag
    24181 atataaaaaa acccgcccct gacaggcggg aagaacggca actaaaactt tattcagtgg
    24241 catttagatc tatgtcgcat ccggcattcg gcacacga
//
LOCUS       DAZGCS010000074        23881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGCS010000074 DAZGCS010000000
VERSION     DAZGCS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.074"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..325
                     /locus_tag="ACHK01_003167"
     CDS             <1..325
                     /locus_tag="ACHK01_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8098084.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(368..1519)
                     /gene="ydiO"
                     /locus_tag="ACHK01_003168"
     CDS             complement(368..1519)
                     /gene="ydiO"
                     /locus_tag="ACHK01_003168"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO8098085.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1533..3128)
                     /gene="ydiF"
                     /locus_tag="ACHK01_003169"
     CDS             complement(1533..3128)
                     /gene="ydiF"
                     /locus_tag="ACHK01_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8098086.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3272..4030)
                     /gene="aroD"
                     /locus_tag="ACHK01_003170"
     CDS             complement(3272..4030)
                     /gene="aroD"
                     /locus_tag="ACHK01_003170"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO8098087.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4061..4927)
                     /gene="ydiB"
                     /locus_tag="ACHK01_003171"
     CDS             complement(4061..4927)
                     /gene="ydiB"
                     /locus_tag="ACHK01_003171"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO8098088.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4939..6204)
                     /gene="ydiN"
                     /locus_tag="ACHK01_003172"
     CDS             complement(4939..6204)
                     /gene="ydiN"
                     /locus_tag="ACHK01_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8098089.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6431..7645)
                     /gene="ydiM"
                     /locus_tag="ACHK01_003173"
     CDS             complement(6431..7645)
                     /gene="ydiM"
                     /locus_tag="ACHK01_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8098090.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFFSFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7745..8101)
                     /gene="ydiL"
                     /locus_tag="ACHK01_003174"
     CDS             complement(7745..8101)
                     /gene="ydiL"
                     /locus_tag="ACHK01_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO8098091.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8237..8344)
                     /gene="rprA"
                     /locus_tag="ACHK01_003175"
     ncRNA           complement(8237..8344)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK01_003175"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8530..9642)
                     /gene="ydiK"
                     /locus_tag="ACHK01_003176"
     CDS             complement(8530..9642)
                     /gene="ydiK"
                     /locus_tag="ACHK01_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO8098092.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10031..13087
                     /gene="ydiJ"
                     /locus_tag="ACHK01_003177"
     CDS             10031..13087
                     /gene="ydiJ"
                     /locus_tag="ACHK01_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO8098093.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQISI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13084..13494
                     /gene="menI"
                     /locus_tag="ACHK01_003178"
     CDS             13084..13494
                     /gene="menI"
                     /locus_tag="ACHK01_003178"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO8098094.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13594..13782
                     /gene="ydiH"
                     /locus_tag="ACHK01_003179"
     CDS             13594..13782
                     /gene="ydiH"
                     /locus_tag="ACHK01_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO8098095.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14330..14698
                     /gene="sufA"
                     /locus_tag="ACHK01_003180"
     CDS             14330..14698
                     /gene="sufA"
                     /locus_tag="ACHK01_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO8098096.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14707..16194
                     /gene="sufB"
                     /locus_tag="ACHK01_003181"
     CDS             14707..16194
                     /gene="sufB"
                     /locus_tag="ACHK01_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO8098097.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16204..16950
                     /gene="sufC"
                     /locus_tag="ACHK01_003182"
     CDS             16204..16950
                     /gene="sufC"
                     /locus_tag="ACHK01_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO8098098.1"
                     /translation="MLSIKDLHVSVEGKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16925..18196
                     /gene="sufD"
                     /locus_tag="ACHK01_003183"
     CDS             16925..18196
                     /gene="sufD"
                     /locus_tag="ACHK01_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO8098099.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPFLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18193..19413
                     /gene="sufS"
                     /locus_tag="ACHK01_003184"
     CDS             18193..19413
                     /gene="sufS"
                     /locus_tag="ACHK01_003184"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO8098100.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19426..19842
                     /gene="sufE"
                     /locus_tag="ACHK01_003185"
     CDS             19426..19842
                     /gene="sufE"
                     /locus_tag="ACHK01_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO8098101.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19991..20995
                     /gene="ldtE"
                     /locus_tag="ACHK01_003186"
     CDS             19991..20995
                     /gene="ldtE"
                     /locus_tag="ACHK01_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO8098102.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21059..21295)
                     /gene="lpp"
                     /locus_tag="ACHK01_003187"
     CDS             complement(21059..21295)
                     /gene="lpp"
                     /locus_tag="ACHK01_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO8098103.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21606..23018)
                     /gene="pykF"
                     /locus_tag="ACHK01_003188"
     CDS             complement(21606..23018)
                     /gene="pykF"
                     /locus_tag="ACHK01_003188"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO8098104.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMTSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAQKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23026..23220)
                     /locus_tag="ACHK01_003189"
     CDS             complement(23026..23220)
                     /locus_tag="ACHK01_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098105.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23575..23784
                     /gene="fumD"
                     /locus_tag="ACHK01_003190"
     CDS             23575..23784
                     /gene="fumD"
                     /locus_tag="ACHK01_003190"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO8098106.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6241 a         5747 c         5997 g         5896 t
ORIGIN      
        1 aatcacttta gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga
       61 atttaccaaa gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga
      121 agcgaaatgg tcactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg
      181 ttatgaaaat gtcgatcact ttgccaaact gtttttgcgc catgtcggtt gttcgcccag
      241 cgattaccgc aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc
      301 tcaaccggta agtcttgccg ggtaaatctg gcggcgcagt ttgttaagaa gctgcgccgc
      361 ctgcagatta tttgttctga taatctttca ggatctgccg acccgcaacg taaatcataa
      421 tttcgtctgt accgccgccg atacgttcac aacggacatc acgccagaag cgggagacgc
      481 gcgcctcatc ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca
      541 ttgcggtacg tgcacagtac agttttgcca gcgccgcgct ggtgcgcagt gactgatgct
      601 gatcggcttg ccatgccact ttcagcacca tgttgcgcat gttgtcgatc ttaatcgcca
      661 tcagcgccag tttttcctgg atcatctggt tatgaccaat gggcttacca aaagcgatac
      721 gttggttggc atagcgggcg gcatcttcaa aggcgcattc ggcaaagccg gtgctgcgcg
      781 cggcgttgat caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt
      841 cttcgcccac catgtcgctc tcttcaacct ccacgttgtc gagatagact tcgcaggtgc
      901 tgagcatatg ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt
      961 cgacccacca cagggtgaag gcttttttgg gatctttcgg ttgcggatcg cgcgccagca
     1021 ccagcatata cgggtactct ttcgcaccgg taataaaggt tttttgtccg ttgatgtaga
     1081 ccttgccgtt tttacgcgtg taagtggtag tggcactgtt gttatctgag ccagcacctg
     1141 gttccgtcaa cgccagggca taggcgggat cacccgtttc cagggtgctc tctgccgttt
     1201 tacgtagctg ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa
     1261 ttaaaaatgc cggagcaccg cattttgaca cttccatcag cgccagcatt tgggtgacgt
     1321 aatccgcagg gataccacca aattcttccg gcacgccaag catggaaata ccgttatccg
     1381 ccagcgcccg cataaactca cgcggatatg tcccgttttg atcgcaggtg cggaaatact
     1441 cttccggaaa gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt
     1501 cagttaaaga aaaatccatc attatactcc tgttaatgag ccgcttcagg caggacaaaa
     1561 cccatcgccg catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact
     1621 ggggtgaagt ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg
     1681 attaaatgca ggccgtcttc tttcagcgtg aatacagcac gttcggtgat gtaacgaaca
     1741 tccagtcccc gttcgagcgc gatttttccg ctaaaagtaa tttccggtag ttcccgaata
     1801 aatttattta cccgtccttc ctggacgata tgcaatttgc catcggcaat ttctgttttt
     1861 aaactgcccg cagttaacgt gccgcagaaa ataattttct tcgaggtggc actgatatca
     1921 ataaatccac cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc
     1981 tggtcgactt cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca
     2041 aactgggacg tcatatccag aatggcgcgg gtattcacgt tcgcgccaaa ggcgataccc
     2101 tgtgaagtaa ttccgccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa
     2161 ccttcttccc gggcgaccag gccaataccg tcagcaatac cgacgccgac attccccacc
     2221 gcgcctttac gcatttcgaa taatgcacgc cgcgcaacta atttacgttg gtttaggggc
     2281 aggctaagtt tggtgctgtc atcaagggtg aagtcaccag aaataaaacg gttaaccggc
     2341 gcaccgccat acagttggct ttgatccgga tcgaccacca caatatccac cagataaccc
     2401 ggaatacgga cagatttagg atgcagcgtg gctttcttaa ccattttctg cacctgcatc
     2461 atcacaatac cgccgttatt gtgcaccgcc tgggcaataa ccaatgcgtc gagatacatc
     2521 acctcatctt caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaggcg
     2581 atatctggcg caatcgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta
     2641 atcaggtctt ctttcgtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat
     2701 gtcccgatgc caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt
     2761 tgtgtaagta caccttgtgg atagttataa gcaataattt tattttgttc tgcgagttca
     2821 gaaatacgcg gcgattgtcc ccagtgacca cataatgccc atttcaccag accttcttgc
     2881 gccagaggac taataccacg gtcggcgcga tcgccaagcc ctgtcggact aataatcgat
     2941 aaattacgtg gcgtttgagt ctgtttatat ttatcagcaa gagctgtaat taacgtggtg
     3001 gcttccagaa taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa
     3061 ttcaccgctt cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca
     3121 ggtttcattg ttaattcctg attcagatgt gattttccct acgccagatg gagaattagc
     3181 gcacagagac tcacgaaata tttttattaa atacggttaa atatattttt taagttcggc
     3241 ggggagggtg ttcccgccga aatattattg cttatgcctg gtgtaaaata gttaataccg
     3301 tgcgtaaatc atttaccgag atttgccctg gtgcagaggc tttttttacc gcaccaaaag
     3361 ttgccgccga gccaaatact tcaccagcca gacgagaaat tacgccagtt tttgccatcg
     3421 acatcgtgat gattggacga tcggcatact gctcctgcat ctctagggtc gcggtaagca
     3481 acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga
     3541 aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt
     3601 catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa
     3661 cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg
     3721 ctgcacgatt gagagcgata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg
     3781 cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgccg
     3841 ccgccatgac agactccaca ttggagaggt cggcaaagtg gtccacacgc cattccagaa
     3901 tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgcgggcg atatctttcg
     3961 ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct tttacagtta
     4021 cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga
     4081 cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc
     4141 cttgccacaa caacatgccg tatccatcaa tcgttttgca accagcttgt tgcgcctgct
     4201 gcaataactt cgtcatatgc gggttataca cgcattcagt gaccagcagt cccggatgta
     4261 acagactgat atcattaact aatgattcat tctcaagggg tttcataccc acttttgtgc
     4321 cattggttaa aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga tcggcgagat
     4381 cggtaaccgt gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt
     4441 tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc
     4501 cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa cagcaccatt gttttgcctt
     4561 tgatatcaaa accgctctct ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc
     4621 cgcgcaggta gccatcatca ttaacgatgg tgttgatggc acccaccagt ttggctgctg
     4681 gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcattgat ataccagttc
     4741 cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaaactatcg ttatccactt
     4801 caaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcag
     4861 gcgataaact gtggcggata ggataggcca tcagcccaat caattcgtat tttgcggtaa
     4921 catccataat taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt
     4981 acgctcgcca aaccgcacat cattctcagg aataataaaa acgcggtaat agcggataaa
     5041 aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata
     5101 ttgaaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc
     5161 acccatcatc atataaatac tggtgacttt ggctttgctt tttgggaaaa actctgacat
     5221 gaccgaaaca ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggcgcc
     5281 ggcattacac accagtggag aagggtacag gtagataatg gctgctgtta ttgttgccag
     5341 tgcagagtta aatacattag cccagatggg ccggaccatt tttttcagta gtgcggcaaa
     5401 aataaagaca cagaccaacg agcccatact gtaataagag atggttttta atgcctcagc
     5461 ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa
     5521 ggtcgagaat gcggctacac cgaacagtac cgatgaaaca ccttccagcc agactaacgg
     5581 tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa
     5641 tttgcttttc aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc
     5701 atagccgtac cagatattat tgagcaacat atagctcacc agcattgggt agaacatttg
     5761 cccaaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca
     5821 ttccatgagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac
     5881 tgccagaccg taggcgagag ttaaattcgg gcaagcggga ataccaaaga agaatagcag
     5941 atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc
     6001 gaagaataaa atactgatca atcgccccaa accgataccg gaaattaagt aggcaatgcc
     6061 cgcgttgtca gtggaaaact tttctgccag agatgacata ttttgggcaa gcgtaataac
     6121 actaatgccg tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt
     6181 gctgaaagcc ttattttgag acattttcac agctccttgc tgaaagataa tttcgttacc
     6241 gtacgtattg ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt
     6301 acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg
     6361 attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa
     6421 gaatattgca ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt
     6481 tgcggctgcg taagccgata aacagtgcca gtaaaaaacc gatggcagcg atggcggtat
     6541 cgaaccacat aatatcggca atacttcttt gggacagatg agccgtaatc aacggaatag
     6601 taaaggtcgc aatactgccc gcactgtaat agatccctgt agctttacct ttagcgtaag
     6661 ggaaacgttc agccattaac gtcaggccaa tttgcacaac acctccagca gaggtaaaac
     6721 caatgacaaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag acggtaaaca
     6781 gagcaataaa tgagaaaaag gtgtacagca tcagtaatgt tgttgggcga acggtattac
     6841 gaatgagtgg agcggtaata aatacacaaa gcagcgaacc cacggtgtag atactgagta
     6901 gtttaatcga catagtgtat gacatgcctg caacaaattg tccgtactgt gccagccact
     6961 ggctaaccag ataaaacgtt gccattgaga tatagccata taaggtataa ctggctaaat
     7021 cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca ggtaagcgac
     7081 gacccggatg gggtgggaac gtacaacggt ataaaaacag agcgttaata aacataatgc
     7141 ctgcagcaat cataaaggac caaccgaacc acagttcagc ccacactaac aggctaatga
     7201 ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt aattaaaata ttggctgtcc
     7261 caggtgagcg tggaaaagct tccatcaaac tgggataagt gcctgcatcg agaaaactgt
     7321 ttgccattcc cgccagaaag ccaaaaacat aggcgataat gatgttattg gtgtgcagga
     7381 tgccaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg cggcgaccaa
     7441 agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg cccagcgatg
     7501 agataactat cgagacaccc gcggcattag tctgccaaag tgtctccagc gaggccatat
     7561 tcaggctcat caaaatgacg cccataccat gcaccaggta attaaaatac aacccaagtg
     7621 cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt aaaaagtgta
     7681 gcgatgttgt gtcagtatcc gttcattatt gttgtaacaa cgacaattcc tttttgtgga
     7741 gaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc gattgataga
     7801 tcttccagtc aataaaattg ccatcagggt agacttgttg aaacgcggtt aagtcgggga
     7861 aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgatg gcatgaagat
     7921 gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacgatca ggaatggcgc
     7981 atttgccatt ttcccactga cgccagcttt cactgtcacc ggtctgggca atccaggtcg
     8041 cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca taagcgttca
     8101 tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca gcctgctgac
     8161 ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagagtgag gggcgaggta
     8221 gcgaagcgga aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag actacacaca
     8281 gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt tgatttataa
     8341 ccgtaagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc tcattagttt
     8401 aaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag caggaaaata
     8461 tggggaagag taagcaaata tcaattagcg ggggttaaca atcaggcggc agagccgcct
     8521 gatgagaaat tacttattcg gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg
     8581 ttggtccgtc ggcggcggga cttcttccac ccacgcggca aacagacgcc aggaaacggc
     8641 taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac caccaataac
     8701 accagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca ttgggcggat
     8761 gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc cccaggtggt
     8821 atcgccagtc cagtagagcc agataatcgc cggaatcagt accggcaacg ggccaagctg
     8881 gacaaggcag gagaggatca ttaacaccgt tagcaacgtt gcataaggta cgccggatac
     8941 ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca cacccagcgc
     9001 caccgcgcgg atagcctgtg ccgccagcag tacggcggca tctccgcgaa cgcctgccag
     9061 acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt acagcagggc
     9121 actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc
     9181 aacgaaccag gtggtggtgg tgccaatata agggcggact ttcgccatga tcgccgtccc
     9241 ccccatatcc agcaagttgt gccagcctgc atacagcttc gcgccaatca ccggaatggt
     9301 attaagccac gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt taattagcgg
     9361 gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa acaccatcac
     9421 taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga tcttttgcaa
     9481 acgtaacaat accggccagg tggcgataac caccgtaccg gcccatgcaa agccgagaat
     9541 aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca gcaccgaaag
     9601 cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt ttcacctttt
     9661 ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc
     9721 aatttcagcg gtttttaaca ggccgattct gcccttaatt ctgatgggcg tgttagaaaa
     9781 aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc
     9841 atgtgctgcg gctacgttcg ttcaccccag tcacttacta ttgtaagctc ctggagttca
     9901 ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aataaacatc acctttcagt
     9961 gcgctattgc agccgcctga aagaatagtg tatcgaccta catcacagac cgcaggaaag
    10021 ggtcaatata atgattccac agatttccca ggcacccggc gtcgttcaac tggtgcttaa
    10081 ttttttgcaa gagctggagc aacaaggttt taccggcgat acggcgacaa gttatgccga
    10141 tcgtctgaca atgtcgaccg acaacagtat ttaccaactt ctccccgatg cggtggtatt
    10201 tccgcgttca accgcagatg tggcgctgat cgcccgtctt gccgcgcagg aacgctattc
    10261 atcgctgatc tttacccccc gtggcggcgg caccggcact aacggtcagg cgctcaacca
    10321 ggggattatt gttgatatgt cccgccatat gaaccgcatc atcgaaatta accctgaaga
    10381 gggctgggtg cgcgttgagg ccggggtgat aaaagatcaa ctcaatcagt acctgaaacc
    10441 gttcggctac ttttttgcgc cggaactttc gaccagcaac cgggcaacgc tcggtgggat
    10501 gatcaatacc gatgcttctg gtcagggatc gctggtctat ggcaaaacgt cggatcacgt
    10561 acttggcgta cgtgcggtgt tgttgggggg cgatattctc gatacgcaac ctttacccgt
    10621 cgaattggcg gaaacgctgg gtaaatccaa taccacaatc gggcgaattt ataacacggt
    10681 ttatcaacgt tgccgtcagc aacgccagtt aatcatcgac aacttcccca aacttaaccg
    10741 ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg agttcgacct
    10801 gacgcgcatt ctgacgggtt cagaagggac gctggccttt attaccgaag cgcggctgga
    10861 tattacgcgc ttgcctaaag tgcgccgtct ggtgaatgtc aaatatgact cttttgactc
    10921 tgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag agacggtgga
    10981 ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg cattccgtca gcgagttgat
    11041 taccgatgtg cctgacaaag agatgctcgg actgaacatt gtggaatttg ctggtgatga
    11101 tgaggcgctg attgacgagc gagttaatgc actctgtgtg cggcttgatg agctgattgt
    11161 cagccaacag gcaggtgtga ttggctggca ggtgtgccgc gagctggcgg gtgttgaacg
    11221 tatctatgcg atgcgcaaaa aagccgttgg tctgcttggc aatgccaaag gtgccgctaa
    11281 gccaattccg tttgctgagg atacctgtgt accgccggaa catctggcgg attatattgc
    11341 tgaatttcgc gcgctgctcg acagccacgg cttaagctac ggtatgttcg gtcacgtcga
    11401 cgcaggtgtt ttgcacgtcc gtccagcgct ggatatgtgc gatcctcaac aagagatttt
    11461 gatgaagcaa atctctgatg acgtggtggc gctgactgcg aaatacggtg gtttgttgtg
    11521 gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg gtgaggaact
    11581 ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg cataaccgac tcaacccagg
    11641 gaagatttgc ccgccagaag gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg
    11701 cggtacattc gatcggcaga tctccattgc ggtacgccag cagtggcgcg gtgcgatgga
    11761 gtgtaacggc aacggtttat gcttcaactt tgatgcccgt agtccgatgt gtccgtcgat
    11821 gaagatcacc cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg tgcgtgaatg
    11881 gctgcgtttg ttggcggatc gcggcgttga tccactcaaa ctggaacaag aactgcctga
    11941 atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc aatagctggc atgcgaataa
    12001 aggcgaatat gacttctcac acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa
    12061 agcgtgttcg acccagtgcc ccatcaaaat tgatgtgccg gagtttcgct ctcgttttct
    12121 gcagctctat cacacccgtt atttacgccc gctgcgcgac cacctcgtcg ctacggtcga
    12181 gagctacgcg ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc
    12241 gctggtgcgc aaactctcgg aaaaacatat cggcatggtt gatttgccgc tgttgtcggt
    12301 cccctcgcta caacaacaaa tggtggggca tcgctcggca aacatgacgc tggaacagct
    12361 tgaatctctc aatgcagagc agaaagcgcg cacagtgttg gtggtgcagg acccctttac
    12421 cagctattac gatgcgcaag tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt
    12481 ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc cagcatatta aaggttttct
    12541 taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta tggcgaagct
    12601 gggtatgcca atggtgggcg tcgatccggc gctggtactt tgttatcgcg atgaatataa
    12661 actggcgctg ggcgaggaac gtggcgagtt taacgtctta ctggcgaatg aatggctggc
    12721 aagcgcactt gagtcacagc cagtggctac agtcagcggt gaatcatggt atttctttgg
    12781 tcactgtacc gaagttaccg ccttgccggg tgcgccagca caatgggccg cgatatttgc
    12841 ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg cagggactta
    12901 cggacatgaa gcgaaaaacc ataaaaattc gctcgggatc tatgagttat cctggcatca
    12961 ggctatgcag cgactgccgc gtaaccgctg tctggcgacc ggatattcct gccgtagcca
    13021 ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg caggctttac tggagattat
    13081 taaatgatat ggaaacggaa aatcaccctg gaagcactga atgctatggg tgaaggaaac
    13141 atggtggggt tcctggatat tcgctttgaa catattggtg atgacaccct tgaagcgaca
    13201 atgccagtag actcgcggac aaagcagcct ttcgggttgc tgcatggagg agcatccgtg
    13261 gtactggccg aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg tgagcaaaaa
    13321 gtggttggtc tggaaatcaa tgctaaccac gtccgctcgg cacgagaagg gcgggtgcgc
    13381 ggggtatgca aaccgttgca tctcggttcg cgtcaccagg tctggcagat tgaaatcttc
    13441 gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt gtgacgttgt
    13501 caccagaaaa gtgtgacgca tattgccttt ggtcattatc tgcacagtaa acggtggtat
    13561 actgtgcagg tcttgtctaa aagcgaggat gctatgtcta ctcaacttga tcccacccag
    13621 ctggccattg aatttttacg ccgtgaccag agcaatcttt cacctgccca atatctcaaa
    13681 cgtctgaaac aactagaact tgaatttgct gacctgctta ctctctcttc tgctgaatta
    13741 aaagaagaaa tctactttgc ctggcgtctg ggcgtgcatt gatcctgact taatgccgca
    13801 agttcgccaa atcgacctgg attaacctcc gtccaggttc gatgaccttc ttaatactgc
    13861 ttatctaccg cctgatattg ttcaaaaata atactaatcg cagtttgtgt taaaacggcg
    13921 ggttagcttt atgagattat tcttatcgcc ccttcaagag ctaagccact gagagtgccg
    13981 gagataagcg ccggatgggg tagaaaccct taagcctgtg ttgcacagac ttaagggttt
    14041 tcttatttct gaacgctgca cttttttacc tatctcttaa atagtcattt tcaatacaac
    14101 tttttctttt tcatcacagc cttaatgctc cttagataat aaccattatc taacaatgag
    14161 atacctaatt cttagcgtgc ctgttaaccc acacatcagg gtctatgctt attaaaagca
    14221 ggcaaaaagc atgttttacg gtaaagcccc tgcgtttgct tggttgaact gataatcatt
    14281 atcactaaca tgctgttata cgctgaaagc gatgaagtga ggtaaatcga tggatatgca
    14341 ttcaggaacc tttaacccac aagatttcgc ctggcaaggc ttaacgctga cacccgcagc
    14401 ggcgatacac atccgtgagc tggtggcaaa gcagccgggt atggtcggcg tgcgcttagg
    14461 cgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc gacagtgtta gcgagccgga
    14521 caaagacgat ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat
    14581 gccgtttatt gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc agatattcaa
    14641 atttcacaac cctaaagccc agaatgaatg tggctgtggc gaaagctttg gggtataggc
    14701 ggtactatgt ctcgtaatac tgaagcaact gacgatgtca aaacctggac cggcggcccg
    14761 ctgaattata aagaaggatt cttcacccag ttagccaccg atgagctggc aaaggggata
    14821 aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg gatgctggag
    14881 tttcgtctaa acgcctatcg cgcatggctg gagatggaag aaccgcactg gttgaaagcg
    14941 cactacgaca agctgaatta tcaggattac agctactact cagcaccatc gtgcggtaat
    15001 tgtgacgaca cttgcgcgtc tgaacctggc gcggtgcagc aaactggcgc gaacgccttt
    15061 ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa
    15121 gaggtggcgg tggatgccat tttcgactca gtttcggttg ccactactta tcgcgaaaaa
    15181 ctggcggagc agggaattat tttctgttcc tttggtgagg cgatccacga tcacccggaa
    15241 ctggtgcgta aatatctcgg caccgtggtg ccggggaatg acaacttctt tgccgcgctt
    15301 aatgcggcgg tagcctctga cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg
    15361 atggaacttt ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt tgagcgtacc
    15421 attctggtgg ccgacgaaga cagctacgtc agctacattg aaggctgttc cgctccggtg
    15481 cgtgacagct atcagttaca cgcggcagtg gtggaagtca tcatccataa aaacgccgag
    15541 gtgaaatatt ccacggtaca aaactggttc cctggcgata acaacaccgg cggtattctc
    15601 aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa
    15661 tcagaaaccg ggtccgcgat tacgtggaaa tatcccagct gcattttgcg cggcgataac
    15721 tccattggtg agttttactc agtggcgctg accagcggtc atcagcaagc ggataccggc
    15781 accaagatga tccacatcgg taaaaacacc aaatcgacca ttatctcgaa agggatctct
    15841 gccggacata gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat
    15901 gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc
    15961 ttcccgtatg tcgaatgtcg caataacagc gcacaactgg agcacgaggc aacgacatca
    16021 cgtattggtg aagatcaact gttttactgc ctgcaacgcg ggatcagcga agaagacgcc
    16081 atctcgatga ttgttaacgg tttctgcaaa gacgtgttct cagaactgcc gctggaattt
    16141 gccgttgaag cacaaaaact cctcgccatc agtcttgaac acagcgtcgg ataaggaata
    16201 aacatgttaa gtattaaaga tttacacgtc agcgtggaag gtaaagctat cctgcgtgga
    16261 ttaagcctcg ccgttcgtcc cggcgaagtt cacgccatta tggggccaaa cggttcgggc
    16321 aaaagtacct tatcggcaac gcttgccggg cgcgaagatt atgaagtgac gggcggcacg
    16381 gttgagttca aaggcaaaga tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc
    16441 atctttatgg ccttccagta tccggtggag attccaggcg tcagtaacca gtttttcctg
    16501 caaacggcac ttaatgcagt gcgcagctat cgcggccagg aaacgctcga ccgctttgat
    16561 tttcaggatt tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga tttattaacc
    16621 cgttcggtaa acgttggttt ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg
    16681 gcggtgctgg aaccggagtt atgcattctt gatgagtcgg actccgggct ggatattgac
    16741 gcattaaaag tggtcgccga tggcgtgaac tcgctgcgtg atggcaagcg ctcattcatc
    16801 attgttacgc actaccaacg cattctcgac tacatcaagc ctgattacgt ccatgtgcta
    16861 tatcagggac gaattgtgaa atccggcgat ttcacgttgg tcaaacaact ggaggagcag
    16921 ggttatggct ggcttaccga acagcagtaa cgcgctgcaa cagtggcatc acttgtttga
    16981 agctgaaggg acaaaacgct caccgcaagc acagcagcat ttacaacaat tgctgcgtac
    17041 cggactgccg acacgtaaac atgaaaactg gaaatatacg ccgctggaag ggctgaccaa
    17101 tagccagttt gtcagcattg cgggagagat atccccacag cagcgagatg ccttagcgtt
    17161 aacgttagac gctgtgcggc tggtatttgt cgatggacgt tacgtgtcag cactgagcga
    17221 tgcgactgaa ggcagcgggt atgaagtgag cattaacgac gaccgtcagg gggtacccga
    17281 cgctattcag gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca
    17341 tatcgccgtg aagcgcggtc aacggccggc aaagccattt ctgttaatgc atattaccca
    17401 gggcgtggca ggtgaagagg tcaacactgc ccattaccga catcatctgg atctggcgga
    17461 aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt aatgatgctc gtcacttcac
    17521 cggcgcacgg ttcactatca atgtcgcagc gaacgcccac ttgcagcata tcaagctggc
    17581 gtttgaaaac ccggtcagtc accactttgc ccataacgat ttgttgctgg ctgacgatgc
    17641 caccgcattt agccatagtt tcctgctggg tggcgcagtg ttacgacaca acaccagtac
    17701 gcaactcaat ggcgaaaaca gcacgctgcg gatcaatagc ctggcgatgc cggtgaaaaa
    17761 cgaggtgtgt gatacccgta cctggctgga acacaataaa ggtttttgta acagccgaca
    17821 gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt
    17881 cgcgcagcac gccatcaaaa cggatggtca gatgaccaac aataatctgc tgatgggcaa
    17941 actggcagaa gtggatacga aaccgcagct ggaaatctat gcagatgatg tgaaatgcag
    18001 ccacggcgcg acggtggggc gtattgatga tgaacagatg ttctatctgc gctcgcgcgg
    18061 gatcaatcag caggatgccc agcagatgat catttacgcc tttgctgccg aactgacgga
    18121 agcactgcgt gatgaggggc ttaaacagca ggtgctggcc cgaatcggtc aacgactgcc
    18181 aggaggtgca agatgacttt ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg
    18241 cgtgaggtaa acggtttgcc gctggcttat ctcgacagcg ccgccagtgc gcagaaacca
    18301 agccaggtga ttgactccga agccgagttt tatcgtcacg gctacgcggc ggtgcatcgc
    18361 ggtattcata ccttaagcgc ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca
    18421 tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa
    18481 gggatcaatc tggtcgccaa tagttggggc aacagcaacg tgcgggcggg cgataacatc
    18541 atcatcagtc agatggagca ccacgctaac attgttccct ggcagatgct ttgcgcacgc
    18601 gttggcgcag agctgcgtgt gatcccgctc aatcccgacg gtacgctgca actggagacg
    18661 ctacctaatc tgttcgatga gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg
    18721 cttggcacag aaaatcccct ggcggaaatg atcacgcttg cgcatcagca tggcgcaaaa
    18781 gtgctggtgg atggcgctca ggcggtgatg catcatccgg tggacgttca ggcgctggat
    18841 tgcgattttt acgtattctc cgggcataaa ctgtatggcc ccaccggaat tggcattctt
    18901 tatgtgaaag aagccttgtt gcaggagatg ccgccgtggg aagggggcgg ttctatgatc
    18961 gccaccgtca gcctgagtga aggcactacc tggaccaaag caccatggcg gtttgaagcc
    19021 ggtacaccca ataccggggg catcattggt cttggcgcgg cgctggagta tgtttcggcg
    19081 ctggggctta atagcatagc cgagtatgaa cagaatctga tgcactacgc gctatcacag
    19141 ctggaatctg taccggatct cactctctat ggcccgcgga acaggcttgg cgttattgct
    19201 tttaatcttg gtaaacacca cgcctatgat gttggcagtt ttctcgataa ctacggcatt
    19261 gctgtgcgta ccggacatca ctgcgctatg ccattaatgg cctattacaa cgtccctgct
    19321 atgtgtcggg cgtcgctggc catgtataac acccatgaag aagtggatcg tctggtgacc
    19381 ggactgcaac gtattcaccg tctgctggga taacagggag gcactatggc tttattgccg
    19441 gataaagaaa aattgctgcg taatttttta cgctgcgcca actgggaaga gaaatatctc
    19501 tacattattg agctgggcca gcgtctgcca gaattacgcg ccgaagaccg aagcccacaa
    19561 aatagcattc agggctgcca gagtcaggtg tggattgtca tgcgccagaa tgcgcaggga
    19621 attattgaat tgcagggcga cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc
    19681 tttattctct acgatcagat gacgccgcag gatattgtca atttcgatgt gcgtccgtgg
    19741 tttgaaaaaa tggcgctcac ccaacatctc accccatctc gttcacaagg tctggaagcg
    19801 atgattcgcg caattcgcgc caaagccgct gcacttagct aaactaaagg gacagctttc
    19861 atccggttgg ctcacattgc cggatggtca atgtttacac tgacctgcac aggagtgttt
    19921 tggtttcagg tgaacataag cgccatccga cgtttcagcg tgagtctccg gcaaatacag
    19981 gaggtttaca atgaaacgcg cgtctttgct tacactcacg cttatcggcg cttttagcgc
    20041 catccaggct gcctgggcgg ttgattatcc gctaccacca accggaagcc gactggttgg
    20101 gcaaaatcaa acgtatacgg tgcaagaagg ggataaaaac cttcaggcta ttgcccgacg
    20161 ttttgatact gcggcaatgt tgatccttga agccaataac actatcgccc cggtgccaaa
    20221 acctggtacg acgataacta ttccttcaca actgttatta cctgatgcac cgcgtcaggg
    20281 gattatcgtt aaccttgcag agctgcgcct ttattattat ccgccgggag aaaatattgt
    20341 gcaggtttat ccaataggta ttggattgca ggggctggaa acgccggtga tggaaacgcg
    20401 tgttgggcag aaaatcccta acccaacctg gacgcctacg gcaggcattc gtcagcgttc
    20461 gctggagcgt ggcattaaat taccgccagt cgttcctgcc ggaccaaata acccgctagg
    20521 acgttacgca ctgcgcctcg cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc
    20581 gccggacagc gttggtttgc gcgtcagttc agggtgtatt cgcatgaatg caccggatat
    20641 taaagccttg ttctccagcg tgcggacggg aacgccggtg aaagtgatca acgaaccggt
    20701 gaaatattcc gtggaaccta acgggatgcg ttatgttgaa gtacatcgac cactatcagc
    20761 agaagaacag caaaacgttc agacaatgcc atacacactg ccagcaggct ttacgcaatt
    20821 taaagacaat aaggctgtag atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg
    20881 gtatccggtt tcggtgagca gtggagcaac tcccgcagcc agcaatgcgc cttcagtaga
    20941 gtcagcgcag aatggtgaac cagagcaagg gaatatgtta cgcgcgacgc agtagcggta
    21001 aacggcagac aaaaaaaatg gcgcacaatg tgcgccattt ttcacttcac aggtactatt
    21061 acttgcggta tttagtagcc atgttgtcca gacgctggtt agcacgagct gcgtcatctt
    21121 tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag
    21181 cgttcagagt ctgaacgtca gaagacagct gatcgatttt agcgttgctg gagcaacctg
    21241 ccagcagagt agaacccagg attaccgcgc ccagtaccag tttagtagct ttcattatta
    21301 ataccctcta gattgagtta atctccatgt agcgttacaa gtattacaca aagtttttta
    21361 tgttgagaat atttttttga tgggaaggca cttatttttg atcgttcgct caaagaagca
    21421 tcgaacgctg ggttttagat aaaaaaattg agagaaaaca ggatgcttcc atcggattca
    21481 tcttagataa agtgagatta tatagtgaaa tccgatttga ttttttattg cttctggtta
    21541 tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa ttcacaaaag
    21601 caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac cagtgcgcca
    21661 gaaaccatga ccacaacatc acctttctga gccagaccgc tctgcaaagc tagttcttta
    21721 cccagacggt agaagtcatc agtagaggtg atttctttca ccaactgcgg cacaacgcct
    21781 ttgctcagca ccagctgatg tgcagttttt tcgttggtgg tcagcgccag gatggttgca
    21841 tccgggaagt atttacgcac cgcacgtgca gatttaccgc cctgggtcgc aacaacgatc
    21901 agcggagcat ccagtttttc tgcagtttca actgcaccac ggcaaactgc ttcagtgata
    21961 cgcagtttac ggctgtcgtt gttgaaatcc agacggctgg tcattacacg gtcagtacgt
    22021 tcgcaaatgg tcgccataat agaaacagct tccagcggat acttaccttt tgcggattca
    22081 ccagacagca taactgcgtc agtaccatcc agaatggcgt tagcaacgtc acctgcttct
    22141 gcgcgggttg gacgtgggtt tttgatcatg gaatccagca tttgagtcgc agtaataacg
    22201 actttacgtg cacggataca tttttcgatc atcatcttct gagcgaagat aacttcttca
    22261 accgggattt caacacccag gtcgccacgc gcaaccatga taccgtcaga cgcttcgagg
    22321 atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat gtggatgttt
    22381 tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg cttacgaata
    22441 aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc ctgtttgtct
    22501 ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc tttgttttcg
    22561 cccaggtcac cgttgttcag cactttacag ataactttgt taccttcaat ggcggtaact
    22621 tccataccga tcagaccatc gtcaaccagt acggtgttgc caacagacag gtcagtagtg
    22681 aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt atcagtggtg
    22741 aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag tttcatggtg
    22801 cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt tttgctcatc
    22861 acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg agagaagttc
    22921 agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc agattcggtt
    22981 ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt ctttaagttg
    23041 agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc cggtattacg
    23101 aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg
    23161 tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc tacagtccaa
    23221 agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt aatccaaaag
    23281 gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat tgttatcaaa
    23341 gatttaacgt tccagggaaa aggttacgca aaaacttcac tgtttgcgca acgtctggtg
    23401 ccgaagttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata tatcatcgct
    23461 caattgctac agttaaaata atttatcgcg ttatttgtct gattttgttt gatttttgtt
    23521 taattggtta gattgatata tgctgatagt tggcatgata aataggtata aagaatggga
    23581 aatcggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg taaagttgtg
    23641 ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg cgtattacgt
    23701 acagcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc gatggaaacc
    23761 gtcattaacg cactggtaaa atgaccattc aggatgaggc ggtaagcttc atcctgatat
    23821 tcttgcttca gttgcggtcc agttaatcat tctaaataac aactgctgaa attatacgaa
    23881 t
//
LOCUS       DAZGCS010000075        22841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGCS010000075 DAZGCS010000000
VERSION     DAZGCS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.075"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..506)
                     /locus_tag="ACHK01_003191"
                     /pseudo
     CDS             complement(37..506)
                     /locus_tag="ACHK01_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1165..1347)
                     /locus_tag="ACHK01_003192"
     CDS             complement(1165..1347)
                     /locus_tag="ACHK01_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098107.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1286..3376
                     /gene="iha"
                     /locus_tag="ACHK01_003193"
     CDS             1286..3376
                     /gene="iha"
                     /locus_tag="ACHK01_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO8098108.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3594..3753)
                     /locus_tag="ACHK01_003194"
                     /pseudo
     CDS             complement(3594..3753)
                     /locus_tag="ACHK01_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3956..4276)
                     /locus_tag="ACHK01_003195"
                     /pseudo
     CDS             complement(3956..4276)
                     /locus_tag="ACHK01_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4699..5274)
                     /locus_tag="ACHK01_003196"
     CDS             complement(4699..5274)
                     /locus_tag="ACHK01_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8098109.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5343..5921)
                     /gene="terD"
                     /locus_tag="ACHK01_003197"
     CDS             complement(5343..5921)
                     /gene="terD"
                     /locus_tag="ACHK01_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO8098110.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(5970..7010)
                     /gene="terC"
                     /locus_tag="ACHK01_003198"
     CDS             complement(5970..7010)
                     /gene="terC"
                     /locus_tag="ACHK01_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO8098111.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7033..7488)
                     /locus_tag="ACHK01_003199"
     CDS             complement(7033..7488)
                     /locus_tag="ACHK01_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8098112.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7511..8668)
                     /locus_tag="ACHK01_003200"
     CDS             complement(7511..8668)
                     /locus_tag="ACHK01_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8098113.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8668..9249)
                     /locus_tag="ACHK01_003201"
     CDS             complement(8668..9249)
                     /locus_tag="ACHK01_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8098114.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            9572..10630
                     /locus_tag="ACHK01_003202"
     CDS             9572..10630
                     /locus_tag="ACHK01_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO8098115.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            10640..11782
                     /locus_tag="ACHK01_003203"
     CDS             10640..11782
                     /locus_tag="ACHK01_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO8098116.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            11775..12548
                     /locus_tag="ACHK01_003204"
     CDS             11775..12548
                     /locus_tag="ACHK01_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098117.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            12550..13629
                     /locus_tag="ACHK01_003205"
     CDS             12550..13629
                     /locus_tag="ACHK01_003205"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO8098118.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            13629..14585
                     /locus_tag="ACHK01_003206"
     CDS             13629..14585
                     /locus_tag="ACHK01_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO8098119.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            14596..15804
                     /locus_tag="ACHK01_003207"
     CDS             14596..15804
                     /locus_tag="ACHK01_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO8098120.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            15822..16289
                     /gene="terW"
                     /locus_tag="ACHK01_003208"
     CDS             15822..16289
                     /gene="terW"
                     /locus_tag="ACHK01_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO8098121.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            16550..16879
                     /locus_tag="ACHK01_003209"
     CDS             16550..16879
                     /locus_tag="ACHK01_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8098122.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            16866..17246
                     /gene="tnpB"
                     /locus_tag="ACHK01_003210"
     CDS             16866..17246
                     /gene="tnpB"
                     /locus_tag="ACHK01_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8098123.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            17289..18119
                     /locus_tag="ACHK01_003211"
                     /pseudo
     CDS             17289..18119
                     /locus_tag="ACHK01_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18150..19763)
                     /locus_tag="ACHK01_003212"
     CDS             complement(18150..19763)
                     /locus_tag="ACHK01_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO8098124.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(19794..20144)
                     /gene="tnpB"
                     /locus_tag="ACHK01_003213"
     CDS             complement(19794..20144)
                     /gene="tnpB"
                     /locus_tag="ACHK01_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8098125.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20141..20566)
                     /locus_tag="ACHK01_003214"
     CDS             complement(20141..20566)
                     /locus_tag="ACHK01_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8098126.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            20620..21371
                     /locus_tag="ACHK01_003215"
                     /pseudo
     CDS             20620..21371
                     /locus_tag="ACHK01_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            22688..>22841
                     /locus_tag="ACHK01_003216"
     CDS             22688..>22841
                     /locus_tag="ACHK01_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098127.1"
                     /translation="MYLHTYQHNDRLPPQKHDKGIIMEFSVIFFWVITGLITILFLKS
                     IITDLIK"
BASE COUNT         5708 a         5834 c         5767 g         5532 t
ORIGIN      
        1 ttctaacaaa gcatttcaac aatatatact tgttaaaaat ccatatcgaa atgccgttgc
       61 agcattaata tatgcctcat tcataaaatg taaaagagca agctcgtacc agtagggggg
      121 aaattcattg acatgtcctg tcaatacgta cagagccctg ccatgcttgc cagcacgtaa
      181 caattccgcc cccagtaacc agcgtgttcc ctgattattc tcagggttat atgttagtat
      241 tttatcaatg agtataacgg catcctgatg acgctgtaaa tgaacattcg ccagaattgc
      301 agcataaaga gcgcaaatgc atatgccaga tgagcgtgaa tatcaatgat gtcaggagca
      361 tgtcaggcaa gacgcctgag tttgttgacg taatttgttt cgtttatttc atcactatcg
      421 tatgcgtcaa gagttttttc aaactcatcc cgaaatgggc caagcctggt ttcaggaaat
      481 aagaaatacc cttggttatt gttcacttaa aagtataatt tgtaattcaa atcctgagcc
      541 tgtaaagcgg ggagtaacat attctattct gaagagaata aaagtcgtga tgcgatgtat
      601 caagcccgga ttgtaatccc agattaacat agatcacaaa acaacttatt cttcactaac
      661 gtcaagataa atatcgttgt atgccttatc acgactacga ataccagcaa gatacaactg
      721 acatacggaa agataccacg ttttttactc ccgaaaataa cgctaaaaag ctacttcccc
      781 atcgtttgtc cttagtattg ccagcgccaa caatgtgggc tgacatgata aagctgtcta
      841 ggaaattgtt cgcctcctca gcggacaatc caaatggtga ttgtctctgt taaacgttta
      901 ttttgaaggt cgactgaata aggtgatgac gctgtagaat ttttcacgtg ccacagaatt
      961 ttgaacgctt tctcttacaa tatttcaatg tttctatcag tattcgccgg aagaagtcat
     1021 cgaccaaatc atcccagtcg tctcgcatca ctgaccattc atggttgaca tgtggtggaa
     1081 atccgcttct acagtaacca ttttttattc gcaaaaccga acacgccata cggatagctg
     1141 ttaactggca tgccgaggca gtcgttattt atatttggtt ttgtcaataa tctttatttt
     1201 ttgtaaaagg caaatataaa ttattctcat tattgtttgt atttgtgtat tgtcttgccg
     1261 gttaacatga tcggagatta gtaatatgcg aataaccact ctggcttccg tagtcattcc
     1321 ctgtctcgga ttttcagcca gcagcatagc tgctgcagag gatgtgatga ttgtctcggc
     1381 atccggctat gagaaaaagc tgactaacgc agccgccagt gtttctgtga ttagccagga
     1441 ggaattgcag tccagccagt accacgatct ggcggaggct ctgagatcag tagagggtgt
     1501 ggatgttgaa agtggtacgg gtaaaaccgg agggctggaa atcagcatcc gaggaatgcc
     1561 agccagttac acgctgatac tgattgatgg tgttcgtcag ggcggaagca gtgacgtgac
     1621 tcccaacggt ttttctgcca tgaataccgg gttcatgccc cctctggccg ccattgagcg
     1681 tattgaggtt atcagggggc cgatgtccac actgtatggc tctgatgcga tgggcggtgt
     1741 ggtgaatatc attaccagaa agaatgcaga caaatggctc tcttccgtca atgcagggct
     1801 gaatctgcag gaaagcaaca aatggggtaa cagcagccag tttaatttct ggagcagtgg
     1861 tccccttgtg gatgattctg tcagcctgca ggtacgcggt agcacacaac agcgtcaggg
     1921 ttcatcggtc acatcactga gcgatacagc agccacgcgt attccttatc ccacggagtc
     1981 acagaattat aatcttggtg cacgtcttga ctggaaggcg tcggagcagg atgtgctctg
     2041 gtttgatatg gataccaccc ggcagcgtta tgataaccgg gatgggcaac tggggagtct
     2101 gacgggggga tatgaccgga ccctgcgcta tgagcgaaac aaaatttcag ctggctatga
     2161 tcatactttc accttcggaa catggaaatc gtatctgaac tggaacgaga cagaaaataa
     2221 aggtcgtgag cttgtacgca gtgtactgaa gcgcgacaaa tgggggcttg ccggtcagcc
     2281 gcgggagctt aaggaatcga accttatcct gaattcatta ctgcttaccc ctctgggaga
     2341 atctcatctg gttacggtgg ggggcgagtt tcagagctcg tccatgaaag acggagttgt
     2401 ccttgccagc acaggtgaaa ctttccggca gaaaagctgg tcggtatttg ctgaggatga
     2461 gtggcatctc acggatgcac ttgcgctgac tgcgggcagc cgctatgaac atcatgagca
     2521 attcggggga cacttcagtc cgcgtgcata tctggtctgg gatgtggcag atgcctggac
     2581 gctgaaaggc ggtgtgacca cgggatataa ggcacccaga atggggcagc tacataaagg
     2641 gattagtggt gtgtccgggc agggaaaaac aaatctactt ggtaaccccg acctgaagcc
     2701 ggaagagagc gtcagttatg aggctggggt gtattacgat aaccccgccg gtctgaatgc
     2761 caatgtcaca ggttttatga ctgacttctc caacaagatt gtctcttatt ccataaatga
     2821 taacaccaat agctatgtaa acagcggaaa ggcccggttg cacggtgtgg aatttgccgg
     2881 cacattgccg ctgtggtcag aggatgtcac gctgtcactg aattacacct ggacccgaag
     2941 tgaacaacgt gatggtgata acaaaggtgc gccgctgagt tatacccctg aacacatggt
     3001 gaatgcgaaa ctgaactggc agatcaccga agaggtggca tcatggctgg gtgcccgtta
     3061 tcgcgggaaa acaccacgtt tcacccagaa ttattcgtca ctgagcgctg tacagaagaa
     3121 agtgtatgat gagaaaggag aatacctgaa agcctggacg gtggtggatg caggtctgtc
     3181 gtggaagatg acggatgccc tgacgctgaa tgctgcggtg aataacctgc tcaacaagga
     3241 ttacagtgac gtgagcctgt acagtgccgg taagagtacg ctgtatgccg gtgattactt
     3301 ccagacggga tcatcaacaa caggatatgt gatacctgag cgaaattact ggatgtcgct
     3361 gaactatcag ttctgataat aacaaaacgc tatcactgac ggtagaatac gttgccactg
     3421 caactcctgg cggaacagtg gcaacgtatt aggttaagtg catttccgat ccgctaatga
     3481 gatttcgtta ccaacaacta atatcgtcac aggaaatgca cggattattt ttaacttatc
     3541 atttacatac ttgtccagag tgtaagcgca ccgtgaagga cgtggggtaa aaattagttt
     3601 acagagagag tgacgttcca gggcaacaac tctttcatgc ggttggcagg ccaggtgttg
     3661 attacactga tcacgtggcg tagccacgtt tccggctcga ttccgttaag ttttgcagct
     3721 accgatcagg ctgtacatca ctgccgcact atcgctcgtc atctcaaagt ccggtctcgt
     3781 cagcaggaag gtatcattct ctcccgccat ttttccaggg gcccggtcag ataagtccct
     3841 ttgtctatcg ctgactcctg actcataacc cggttagcag aatgcagggt caccacctcg
     3901 ccacgaccaa atccaaataa gtcaattgca ccttctcaat cgccattttg tcagtaagcg
     3961 tacagcctga actgatggta tcttcaccat caatgacaac ggtgatcgca attttactga
     4021 cgttcgccgg aacacgatcc agtgctatct caatgctggc ctgctgcgaa ccggtaacga
     4081 gcctgactgc cccctcagga gaagacaaat tattataaaa gataaagtca gaatcgccac
     4141 tgacctttcc ctgagcatta agcatgaaca ggcaggtatc gggttcgcct ttaaagccgg
     4201 atttggctgg gtactgaaga ttcagcctga tcgtagattg ctgaaggggt atgttttgtc
     4261 cggattgtaa attcatatta actctcctga tttttgatta ttattaatgc gcagcgttta
     4321 tatatgttcc ctaggcttag ttctggacgc tggatattcg gtgaggcgta aatatggtat
     4381 gacaccattt ttcataacgc tgaagtttct atacctgttg aatttgaatt ttcattgacc
     4441 gggtatctta ttttccaggg cccgttcctt cataagtcgc aaaagtaaca tatatccgaa
     4501 gggcatgctg ttgatatcag acacggaata ctggctttaa ccagcaacca cagataaacc
     4561 cggggcctgc ataaaaggtt catgccagaa ttaacatagc tctgctttct gccccccccc
     4621 tttcccatat tcacccgttg atagcggatc gatacccaaa aaaaaccccg gcattgccgg
     4681 ggtcagactc agcgtctttt agacattgac gccgtgctgg gaagcaagcg ctgacagacc
     4741 accggcaaaa ccctggccaa cagctttaaa cttccactca gtaccatggc gatacagttc
     4801 accgaagacc attgcggttt cggttgaggc gtcttcagac agatcgaaac gggcaatttc
     4861 cgtcccgttg tcgttgttgt aaacgcgcat gaagctgttg ctcaccatgc cgaagttttg
     4921 tttacgcgct tctgcatcat agatggtaac ggcaaatacc agttttttga tgtctgctga
     4981 gactttggtc agatcgattt tgacctgctc atcgtcgccg tcgccttcac cggtacggtt
     5041 gtcgccctgg tgctctactg cgccatcagg gctggtttta ttattgaaga aaatgaaatg
     5101 ggcatctgac agtactttac cgtcttcacc tactgcgaat acggaagcgt ccagatcaaa
     5161 accctgacca tcggttacac gggcatccca gcccaggcca accatagcga cattcatggt
     5221 tggtgcttct ttggtcagag atacgttgcc gccttttacg agagaaactg ccatttttag
     5281 ctcctgcaaa cagttgaatg aggctgaatt aacagcccca gatatgaaaa gttacttttc
     5341 gatcaggacg cgttaatgcc gtactgagca catacagatg ccagaccacc agcataaccc
     5401 tggcctactg cgcggaattt ccactcacca ttgtggcgat acagctcgcc gaacagcatg
     5461 gcagtctcag tggacgcatc ttcggtcaga tcgtagcgag cgacttcagt ctggttatcg
     5521 tcattaacca gacgaataaa cgcaccggat acctgaccaa agctctggcg acgagcctga
     5581 gcatcgtgga tggtcacaac gaagatgatc ttgtcaactt cagacgggac ggcgtccagt
     5641 ttaattttca gcgattcatc atcaccatcg ccctcaccgg tgcggttatc gccggtgtgc
     5701 gttacggaac cgtcggatga cgtcaggttg ttatagaaga tgaaatctga atcgccgcgc
     5761 actttgccgt ttgaggccag caggaatgct gaagcatcca ggtcaaagtc ctgaccgtct
     5821 gttgaacgcg catcccagcc aaggcccacc aggacatttt tcattgacgg agctgcttta
     5881 ctcagggaga cgttcccgcc tttggaaaga gaaacactca taaaataacc tcttcgatta
     5941 gtaattgttc aggttaacac ttaaggggat taactcccct tttcctcaga ttcaggtttg
     6001 cccgggaaca tgacgcttgc gagaatgccc agcgccagta cacccagtac aacatacagg
     6061 ctggttgttg ccgcgatgct gtaaccatga tgccagatgt gatcggtcgc attcaggccg
     6121 agttttgccg cgatgaagaa cagcagcacg ataacggcct tctccagatg aaccaggtac
     6181 tgtttcagtg cctcaaggac aaaatacaga gtacgcaggc ccaggatagc aaacatcatg
     6241 gcactataga cgataagcgg ttcacgactg acggcaatga ttgccggtac cgagtcaaac
     6301 gcgaacatta cgtccgagag ttcaaccaca gccacacaca ggaacagcgg ggtcgcatac
     6361 agcgccgctt ttttaccacg gccaatggtg acatcgctgt tttctggttt cgccagttca
     6421 gcatccactt ccttctggtt aagtaggaag gcatgccctc tgagcttcgg ccagataggg
     6481 aagaagcgtt taaccatacg gtaagccaga tgctgggagt aatcctcaat ttcatcatcg
     6541 tcatcaccgc ttttaagcat catgaccgcc gtccaggcaa cgataatagc gaagacaact
     6601 tcaacgtacg gccccagact cagcaggctc gtaccgatgg cgacaaagat gcccctgaag
     6661 acgatggcac caatgatccc ccagtagaga acacggtggc gataacgatc cggaacggcg
     6721 aaccaggaga agattgccat catgacgaac aggttatcga ccgacagcac tttctccagc
     6781 gcataacccg tgacaaacag actggcaacc tcagcaccgt ggtggatata gaggaaaccg
     6841 gcaaatgcca tcgcaaccac aacccagaat acggaccaga gcgcagcact cttcagcgag
     6901 attggcttgt catcacggtg cataaacagg tcaataaaga tggcaccgac tgaaagcgca
     6961 ataaaaacaa tgaccgtttc agtcgggaag ccgatgtgtg tggaaaccat attttaccct
     7021 tagggatgtg gatcagaggc caaattcagc cgggtcgaag cccaacgcga tagcgatctc
     7081 gcgggaggcc agtttctcgt cctgatcgaa gttaccgtca cttttcgcaa cggcaatacc
     7141 aacacgtaag gccagctgag cggcctcagg ctgatctttc agcgccagga tgtatttcat
     7201 ggtttcgccc ttgccaattt caacatcgaa atcgaagctt gaaaccagtt tattgaagaa
     7261 ctcaatcacc tcattggtat cgaagacctt cagctcttct gaagagcgca gaaagcccat
     7321 cattttctgc ttttcttccg aacttacgcc gtcactcgat acggcaatac gggcacatac
     7381 agcaacggtg ccctgcatga attttttgtt tttgaaacgg ccaacctggc gggtcagttc
     7441 gtcacggcct gagttaatgg cacctttaac tttgtcgaaa aagctcatac ctgtttcctt
     7501 tgttgctgtg ttatttagaa ccggcgctcc agcgaaatcc ccagccaaat gcccggtcca
     7561 tttcgtcctg gcctttgaag tactggttaa ttcgctcgac tttaattgcg ccgttttcgt
     7621 ttacgagtct ggcaatggcg cacaatgtgc gacggttctc accttcggtc aggcgggtct
     7681 caatgggcgg ttgatccggc acatgaatgg tgactacacc atcagtcttg tcccagctgg
     7741 gaacaccttc ataaataaag gcgtaaatca gcacttcgcg gatatgcttc cattcacgtc
     7801 cattgatatg cagccactcg ccgtctgata catcacccgt ccggtcatcg cccttgagct
     7861 gaacatacgg ttcatcgcga taatcaccga atgcattacc gagcgcctga atgaccgatt
     7921 tatacccgtc ctgaagctca acaaaggccc ccagatccag gtcgattcct ttggagccga
     7981 acatacctgc aaagcctgat ttgctactgc cccggtgcca gttcaggttg atccggattt
     8041 caccgaagtt atcccgtttg gtcaggctga ttgcaggctt ctcttttgtc agcgatacct
     8101 tgctcaggct gatttttgtc tcaactggcg gaggggttac aaccggagga gttggggctg
     8161 cgggagccgg ttcgtcggca atatttacgc cgaaatgctc tgccagcggt ttaagtccac
     8221 cgttgaaccc ctgcgcaacg aagcggaatt tccagtcatt attacgacgg taaaattccc
     8281 ccagaattaa ggctgcttcc tgacggccgc tcagttcgac actgccgcta accaggccgg
     8341 tagccccttg ctccacgtcg atggaaagat tacgaagacc ggacaccgtc tgcccaccgt
     8401 cacaggtcac ggtgaacgca attttctgga catcaggttt cagccggttg agcgcgacag
     8461 taaaggttga gtactggcct tcactgacca ggctgacggt accatcatcg ttacgtggct
     8521 gaccataaaa caccatgtcc gcatcaccct gcaccttccc gtcggcatac aggcgaaacg
     8581 ccgaagcatc cacctggccg cccgaggtga tccggacccg caactcctgg gctggaacgg
     8641 gggcattgcc ccccggcgtc aggttcatta gcgaataacc gctgaaacga tatcggagtg
     8701 catatcgtca attgtgcggc cgcgacaggc atggccaagg gcggtaaagt cccagttgcc
     8761 gttattacgg cgcagcgaag aaataacgat cccggtatgt gagccctgct cagtcagctt
     8821 gtagcgagcc agctctttgc cggtttgatc gacaacgcgg cagaaagcgt tttcgacgtc
     8881 attgaacgac tgaccacgga agctgtttac cgtaaacgcc aggtattcaa cgtttgcagg
     8941 caggcgggag aggttgacct taatcacttc atcgtcaccg tcaccctcac cggtcaggtt
     9001 atcgccactg tgcacaacag cgccgcaggt ggatttcagt ttgcggaacc agatggtgtc
     9061 aattgtttta cctgtgctgt ccattaatac acagccagca tcgagatcga ttgagtcgtt
     9121 gcctccaaac aatcctccaa gaagaccttt tttcttaacc ggatcccaac cgagaccgaa
     9181 atgaagctgg cttaatgcag atgattcttt gctgagtgat accgtctggt tcttaactaa
     9241 tgaaaccatg ttttattcct taaaatggta atgaccgaga caatcaaagg tatatttagt
     9301 aactgacgaa gaaaatcata tcatgatgaa gttctctgac aagggaaatt tttgtggttt
     9361 tctgtaaaaa cacaaaaaga aatccttttc agggtgttag tttttctatt tttaatttaa
     9421 acatcaatgt gttataagga atccaaaaaa cactgacgca ctccataacc acaggagtta
     9481 ttaatcatat tatgattgac atgtagctta gacactcata gactcacttc tcaatcataa
     9541 atttgcatgg cgttccggat aattaaaaaa aatgaacaag aaaatttggt ttatggaagg
     9601 tttatcctcc cagcgagata ttattcaggg ggtaaaatca tttgcacaaa aaaataattt
     9661 tgccattacc gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt ccgttgccga
     9721 ttattctttg actgaacctg aagatcctca aaaacgtctt cagtttatcc aggaaaccat
     9781 tcagacctac ggcatccacc atattcatac tggccgtaac agccagtggt ttgaagaaca
     9841 ccgttcagcc attgaaccga ccggtgccac ccttactacc ggtgcaacgg gcgtcgactg
     9901 gttaactctg gctgacgaaa aagttacttt tgctcagttt atggagcaaa agggtctccc
     9961 ggtcgtacca tcctggcggg tgaatacgct ggcagaatta aagacacacc tcgcggcccc
    10021 gccgttcact gacagcccgg tatgcgtgaa gccggtgacg ggtatctatg gcatgggatt
    10081 ctggcgcttt gatgacagtg cttcgcctat ggccgtcttt aatcatcccg aacatcgtct
    10141 ggtcagtccg caacagtata ttgcagcagc atcagctgct gagtcgttta aaccccttgt
    10201 tttgatgccg tacctgccag gcccggaatt ttccgtcgat atcctcgcgg ataagggcga
    10261 aatactcgca gccgtgggac gccgtaagga aggggctatc cagtatctgg taaacgaagg
    10321 aagcgcctgg gaactggcgt gtgactgcgc ccgtgttatg aaggccgacg ggctggtgaa
    10381 tgttcagacg cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca acatgcgtcc
    10441 gtcagggggg gtgggttata cccttcacag cggtgtgaac cttcctgggt tatttgctgc
    10501 ctttaagctc ggtctgatgt ctgaagatat ggtacgccag agcgctaaaa acaccttttc
    10561 tccggttgcg gtgagatcca ttacggatgt aattgcatac ccggaatcac tctctaacct
    10621 tctgaattaa ggcgtattta tgtctgatat cctcgaagat atgatttatc gccgtaccct
    10681 ctcatgcggt acgattcagg taacccgtga ccagggtgaa gtttcgctcg atgacctgtt
    10741 tgacatcgct gaaagacgta acccgaaacg cgcctttctg tttgtcagca aagtactcgg
    10801 caggcacatt cccgtgccac cctcagtcat gcgacaggcc tacagacagc ttgccagcca
    10861 gttcccctcg acactgacag gacccgtact gtttatcggt atggctgaaa ccgccgttgg
    10921 gcttggggcc ggtgtatttg atgaagtgcg ccaccagcat cgtgaatctg tctatctgac
    10981 ctctacccgt cacccggttg atggcacgtt gctttgcgag ttcaaggaag aacacagcca
    11041 cgccaccgat catctgatct atctgccaga tgatgaagag aaaagacgtc gtgttaccaa
    11101 cgcacgaacg ctggttttga ttgatgacga ggcaaccacc ggtaatacct ttattaacct
    11161 gctttctgcc ctgcgtaata ccggcaagct tcaacatatt gaacaggtta tcgccgtaac
    11221 gctgaccgac tggagtggca atgccctgtc ggagcgcacc cccttgcccg tcacttcggt
    11281 ttcgcttgta agcggtcagt ggggatggac cccattacct gatgcacctg tcccggacat
    11341 gccgaaagtc aacgtaactt cacgaggaga atgggacatc caggggaaac agtcatgggg
    11401 ccgactgggg atgctcgcac ctgcggccga tctcggccat gaggtctccg tccacaaggg
    11461 ggaacgtatt ctggttctcg ggaccgggga attcgtctgg gagccgttcc tgcttgctga
    11521 acggctcgaa gctgccggag cacaggcatt ttatggatcg accacccgct cccctgtcgc
    11581 cgttggttat gccattgagt ccgccatttc ctttacggat aactacgggc tgggcatccc
    11641 caattttgtc tataacgtcg cccaccagca gtttgaccgc attcttgtgt gtactgagac
    11701 acccgcagaa agtattgaca cgcagcttct taaagcgctg gctgaggttg cgcccgtcgt
    11761 ggagattgtt acctatgaat aaacccgttg ttctgagcga ccttgacgac acgctctttc
    11821 agacccgtcg taaaatggtg gacgagctgg ccctggagcc attccgtacc ggtgccgttg
    11881 atcgcaccct gaatccacga agctttatga cggaagaaca gtccatgttg gtggactggc
    11941 tcctggagca ggctgaactc atacccgtta ccgcacgggg aactgaagaa atcagccgcg
    12001 tacggatccc tttccactcc tgggcaatca caacgcacgg ggccgtcatt ctcacgcctg
    12061 aaggcaaacc cgacgaggag tggaaggccc atatgctcgg ccagctggct ccctaccagg
    12121 aaaagctgac atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa ggaatcaatg
    12181 cctgggcaag gctgaacttc gaatacggtg aaacggcggt ttatatggtg atgaaacacc
    12241 gcgacagcac ccgccttgac gagctcaatg ccattgcaga tgagatagaa acggtgtttc
    12301 cgaccgaggg cttctacatc caccgcaaca gtaataacgt ggcctggctt cccaccccgg
    12361 ttgagaaagg gctggcagtc agctggctgc ttgaaaaact tcgggctgaa cgaggagttt
    12421 tccccgtaat tggtttgggc gacagcctga gcgatcatcg ttttatgaaa ctgtgcagct
    12481 ggtttggcat cccgcgtcag agtcagtttg cagatgccat ttcacagcga atttttggag
    12541 aaaattaaaa tgcagccaca tgaaacattt accggctcat accagcccgg tgacgtggaa
    12601 tttctgctaa agccggttgt cattgagatg acgccggttg agaaaaaaga agaactgatt
    12661 cagtccggga agaaacatta ttcggacatg ctcagccagg agccagcgcc tacacaatgg
    12721 catcttgatt tgtttcaccg ggcgctggat cggggcgcag agaggcttgc aaaagaagtc
    12781 acacagctcg ctattgctct ggccgaacgc ttcggtgatg agcccattgt actggccagt
    12841 cttgtcaggg ctggcgttcc gctcggcgtt atgctgcacc aggccctgcg tgacatgggg
    12901 aaaacctcat ggcattacgg tatcagcatt atccgggatc gtggaattga cggtgcagcc
    12961 ctcgacgtca ttgaagagcg gcatggtacc agcggtattg tctttgttga cggatggaca
    13021 ggtaaaggcg caattaccgg agagcttgta cgcgcactga aagatcgccc gggctatcct
    13081 gagcagccgc gactggttgt cctggccgat ccctgtggct gctcctggct tgcagccagt
    13141 gatgatgact ggctcattcc ttttggcatc atgggggcgc cggtatcagg cctgatctcc
    13201 cggtcagtat ggtcctctga aggattacac gggtgcatgg tttgcgagca tctcagtgaa
    13261 ttcgaatgca gccggatgct tgccgatacc gtcgctcatt tccgtaagaa gttaacaccg
    13321 tcatccctcg ctcccctgag ctggaatacg gaatcagccc gggtcttatg gcagacaagt
    13381 cgcgacgtta tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa tcgtattaaa
    13441 cccggtattg ctgaagcaac ccgggctgta ttacgtcggg taccggacca tgtatttgtg
    13501 cgttctattg acgacccgga cgttgccttg cttgtagggc ttgctcgtga aaagggaata
    13561 gttgttacag aaatgggggg aaccctcggc cagtatcggg ctgtaaccat tatcaagaag
    13621 gtactctgat gaaaaatcgt ctttctccct ggaatctggg agccacgcta tacatgcctg
    13681 caacacggga agatattgct gatgccgtcc tgcacgggaa aatcccgggc ttgcgctctc
    13741 tggtgatttg cctggaggat gctgtcagtg aagcagacat ccccgtcgcc ctcaaaaatc
    13801 tggagcacct gctgcacgag ctcagtaaca gcatgagtag cctgggtaaa aatgactggc
    13861 ctctggtgtt cattcgcccc cggcatgccg aaatgggcag atggttaaaa gcgcattatg
    13921 atctttccgc tgtcgacggg tttgtgctgc ctaaatttac cctcagttcg ttagctgaat
    13981 ggtgggatat catggtcggg actcacctgt gcatgatgcc aacgctggaa acagaagatg
    14041 tctttgacgt ggttcagatg cgcgagctgg ctacccgtct ggaggaacat ccctgccacg
    14101 accgcattat tgcgcttcga atcggcggca acgatcttat gaacgttgtg tcgcttcgcc
    14161 gtccccggga cctgacgctg tatgacagcc cgatgggcta cgtcatcaaa atgctcgttt
    14221 cggtcttcgg cccgcgtgat tttgccctga ccgcgcctgt atgtgagcat attgacgatc
    14281 atgccgttat ggccagagag ctggctcttg atatggcaca cgggcttgtt gggaaaacgg
    14341 ccattcaccc gggtcagata gaggtcattc aaaacgcgct gatggtcact cagggtgagc
    14401 attctgacgc tctgcggatc ctgaactcta cccaggccgt gtttaagtcg cagggagcaa
    14461 tgtgtgaacc cgccacacat cgccgctggg cggctggcat tctggacagg gctcgttttt
    14521 atgggttaca gaacgagcaa agcgctgatg gaatcagatt acttaccgtg acccagcatc
    14581 attaaggaaa tcataatggg gttcaggttc agaaagtcga tcaacattat tcctggcgtt
    14641 cgcctcaacc tgagtaacgg tgcaccgagc ctgagtgtcg ggccgagagg tgcttccgtt
    14701 tcttttggta gccgggggac ctatgccaat ctgggcttgc ccggtaccgg gctgagttac
    14761 cgtacccggc ttgaccgggc cgcgcgttcc agaggtgaaa accggacggc aaccgacccg
    14821 gggctcagac aggcgcttga gcagaaagcc gctgaactca tgtcagcggt aaccgcaatc
    14881 cgtaatattc acgagctgac gccggatcca aaaacaggca tcagctgggc agagctggaa
    14941 gcagtatacc tgcataacag aacgtcgcct tttcaggttc cggcaccggt gcgtccagaa
    15001 aagccagact accttgtatt gccggaaaag cctgccgaga gcgaaggcat tagttttttg
    15061 ggtaaatggt ttgaatcgga atcagctaaa gctgagcgcc acgccgaaaa tcttcgccgg
    15121 tggcagcagg agctgattga tgtggagcgt gagaataccc ttcgacagca ccggtaccag
    15181 caacaacgga cggcctgggc cgaacagtat gcaaactgga agtttgaagc tgaagaacat
    15241 gaaaaacggc tcgccacggc tcaggcagat gcccggcagc agttccggac agacgccgcg
    15301 tttttcgaat catacctggc gggtgtgctg gcagaaactg aatggccgcg tgaaacgctt
    15361 gttgcatttg aagtaaagcc ggagctatca gcagtcctgc tggacgttga tttagctgag
    15421 attgaagatt tccctgataa gatttacggc gttaatgccc ggggaacgga gctgacggaa
    15481 aaagccatga cgcaaaaagc cgtacgcgaa aactatgccc accacgtcca tggctgcttg
    15541 ttccgcctgg tcggtatcgt tttacatacg ctacctttcg acaacgtgat tgtgtcaggc
    15601 tttacgcaac gggtcagtaa gcggaccggc tatctggagg atgagtatat cctgtcctgc
    15661 aaatgcactc gcagccagat gtcgtcagta aattttgcag gcataaaaca cattgatccg
    15721 gttgaagcgt taggcgatca accggttatt cgaaagatga gcagtacctt catttttcag
    15781 cctattgaac cactaaccct ttaagcaggc taaatcttac gatgcaatta aataccagac
    15841 aggcccggat ttttaagctt gccaacttac tgggcaccgg caagcccgtt tccgctgccg
    15901 acattataac cagtctggaa tgctctgagc cgacattaac ccgggccctc aaagagctac
    15961 gcgagtcata ttctgcggag atcaaataca gcaaggcagg tcattcctat catctcgtta
    16021 atccgggcca gttggataaa aagaccctcc gccggatgaa tgaagcgctc gcacagaatg
    16081 ctgaactgaa aaccggtgag tccacaggga aggtcgtact cgataaggat aaaaaaacag
    16141 ccgtgtcatt atcgctacgc atgcggattc taagaaaaat tgaccgtctg gctgcgctga
    16201 gtgactcgac ccgaagtgaa gcggtagaga agctggctct gcactccgtt gatgaactga
    16261 taaaagaata cagcgctaaa aagtcctgac gctaaatctc agaccggggt gaaaactccg
    16321 gttttgtcat atttcacctc tgtattggcg tgatgcaaaa tggcatctga atcgatcatg
    16381 gtttggcatc catttccgat cacagtttgg catcacaatc gatcacgatt tggcatgctt
    16441 ccgctcattg attggcatcc tggtgtcgtt acggggaagt catcttcaca gggatcttca
    16501 gtcagttagc attaccgaca tccattcacc aacttaccgg agataatcaa tgtcaaaacc
    16561 ccgatggacg cttgatcaga aaaaacatca cgttgccgca tggcgcgcca gcggccttac
    16621 ccgtgaacag tactgtgaac tttacgacat tccgtttaaa tccctgcgcc agtggccgca
    16681 ggatgtcgca aaagccgaaa aacgagccag agcaccagaa attattcctg tttcagtatc
    16741 tggctcttcc ggaatgactg atggcagacc attgtcggat gagcctgtca ctctcttcct
    16801 gcccggtggt atccggatgt gctgccagcc ttctcagtta accgatgttt tccgggctct
    16861 tcgccatgct gacgcctgat aacgtattcc tggtcgtaaa acccgtcgat atgcgccggg
    16921 gtatcgacac actgacacag tacgtccaga acgaacttaa cgctgcatgg catgacggtg
    16981 ctgcttttgt cttcaccaat aaagtccgct cccgcataaa agttctccgg tgggacaaac
    17041 atggcgtatg gctgtgtacc cgacgactgc accggggaag cttccgctgg ccccggaaag
    17101 gtgatgccac atggcatctc acacaggatg agtttcactg gctggtcttt ggggttgact
    17161 ggcaacaggt gaaagggcat gacctggcaa aatgggtata ccaggaagaa cccgaagatg
    17221 ctcagagcaa cgcaataact caatgaaata taatgaatat ccatgcgtga tgtcatataa
    17281 tccaccgcat ggatatctcg cttctcagta ccaccagtga ccctgaacag cttcgcgctc
    17341 tggcaattgc catggtgcaa aaagtcatgg ctgagaatgc agaacttcag aaccgtatcc
    17401 gcatcctgga agagcagatg aagctcgcac gccagcagcg tttcggtaaa aaatgcgaat
    17461 cgctggccgg gatgcagcgt tccctgttcg aagaggatgt tgacgcggat atcgcagaaa
    17521 tctcggcgca tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa acaacaacac
    17581 gtcctgttcg taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt atcccgccag
    17641 ctgaggaacg ttgtcctgac tgtgatgccc ccctgcactt cattcgcgat gaagtcagcg
    17701 agaagctgga atatattccg gcacaggtcg tggtcaaccg ctatatccgc ccgcagtaca
    17761 gctgtccctg ctgtgagaaa gtgttcagcg gaaaaatgcc ggcacatatc cttccgaaaa
    17821 gtgctgttga gccatcggtc atcgcacagg ttgtcatcag caaatatacc gaccatctgc
    17881 ccctgtatcg ccagcagcac attttctccc gaatgggggt ggagctcccc gtcagtacaa
    17941 tggcggatat ggtcggtgtt gctggcgctg cactggcccc gctggcaaaa ttgttacgcc
    18001 acgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata
    18061 cccggaaagg cggtaaatcc tgctccggct ggctgtgggc ttacgtcagc ggagaaagag
    18121 taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagtactac gttccatggc
    18181 aggagttcat caactcggtt ggaaggccat tctggcagta cgctcaggat atggcgcaga
    18241 tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta caacagtgct
    18301 ccacgctcgc cgccgtgatc gctgccaaag aacataaagt ttttctttcc gagacagact
    18361 gcacgaagcg ctctttccgc agcattatta tccgcctccg ccagaccgtc atcactgtaa
    18421 taacagaggg catcccactg attcagtaca tagctgcacg cttcgcccag tctggatttt
    18481 ttcgacagcg taccattctt ctccaccatc cattcatgca gtgacgtcag taacactttg
    18541 cttcgctgct gcctgacggc aagacgctct gactccggta atccccgtat ttcatcctcg
    18601 atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcact tttgctgctg
    18661 atgtatacat cgtggatttt tcgccgggca tgggcccagc acgcaacttc tgtcagtgca
    18721 ccaccttcac gttctgcact gaacagccga tcgtaaccgc tgaacgcatc cgcctgcagg
    18781 ataccccgga agggacgaag gtgttgtacc ggatgttttc cctgcctgtc tggtgagtag
    18841 gcgaaccaga ccgccggtgg ctctgatgag cccgcattcc ggtcatcccg gacatacgtc
    18901 cagatgcgtc ctgtttttgc cttttttctg cccggtgcca gcacttttac tggtgtgtcg
    18961 tcagtgtgaa ccttgcgggt gttcatcacg taacggtaca gggtatcatt cagcagagtc
    19021 attaactgac aacatgcgtc aacccagttg gagagtaatg cccggctcag ttcgatgccc
    19081 tggcgggcaa agatttcact ctgacgatac agtggcaggt gttcgcagta ttttcccgtt
    19141 aacacgcggg caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg ggacggcgct
    19201 ggcgcttcaa ctatacagtc acattttgta caggcttttt ttacccgaac agtgcggatc
    19261 actttcaggg cgctactcac cagttccagc tgctcagcac tgacttcccc cagataatcc
    19321 agctcactgc cacactccgg gcaacaactt tcttcaggct ccagacggtg tatttcacgg
    19381 ggaagatgtg ccggtaacgg acgacgatga cgtgactgtc gcaactggcg gggaacctgc
    19441 gggtcatcct cacgcccact gtaacgatcg ctttcctgtt cgcgttgttt cagttgggcc
    19501 tcagcctgtt caacctcacg ctgcagtttt tcagaacggg taccgaacaa catccggcgc
    19561 agtttttcta tctgggccct cagatgttct atttcccgct cctcctcttc gatcttttcc
    19621 tcggcacgtg ccagtgcaga gcgcaggaag gcctccgtct cttcaaccag actcagttgc
    19681 tgatctttct gacggagggc ttcagcctgc tcagagagca gcctttccag ctcagtgata
    19741 cgaatgaggt atttccgact catgaccgtt tttataatcc ggccatgaca tttttacaac
    19801 attgtcagtg cattaaggcg ggatgttttg ggctgacgcc agtccagttt atcgaggagc
    19861 attgccagct gcgagcgggt aatggatacc ttaccgtcac gcaccgcagg ccagataaac
    19921 tggccttcct ccagacgttt ggtgaacagg cacagaccat cagcatcagc ccacagaatt
    19981 ttaatcgtgt caccccgtcg gccgcgaaag ataaacaggt gaccggagaa ggggttctca
    20041 tccagcacat gttgtacctg ttctcccaga ccgttgaagg atttacgcat atcagtaacg
    20101 ccggcaacca gccagattcg agtgcctgat gggagcgaga tcatcgtccc ctcccggtca
    20161 gttcacggat caacaccgtg agcagctctg gtgaaggatt ttccagcgtc atgttaccgt
    20221 ggcggaactc aactttacag gaactggcac tgactgtgct ttgtgaagga gtggataaaa
    20281 gcggagtaag agccgccata ggctctttct gctcatcagg cgttatctca acaggtaata
    20341 attcaacgcc agcgccagaa gaggttgtta ccggaagacg ccgcgatata cgcctttcgt
    20401 tctgccagag cctgagccat ttgaacagga ggttatcatt gatatcgtgt tcccgggcaa
    20461 tacgggcaac agaggctcct ggttgtgaag ccagtttaac catttgaagt ttaaactcat
    20521 ttgaaaatga tctgcggggt tctgaggata atattttctg ttccataaca ggtgtccact
    20581 agttgaaaag gtgggcacct acgttaccaa tactggctta atggctacat acggcggtca
    20641 gtttacgctt acgagaaaga agcggtccgc cggtcgtctg ttttgactcg cagaccggac
    20701 gggccctcag atatcctgag gcctggctgc agggctggtg tggcggtacg ctggtgagtg
    20761 acggatacag cgtgtacaaa tctctggcgg ataatgaccc cggtattaca tctgcctgtt
    20821 gctggagcca cgcaaggcgc ggtttcgcaa atctgtataa ggccagcagg gagccccgtg
    20881 cagccatggc gctgagaaaa atcgcgggtc tttatcgcat tgagaagttc atccgtgaac
    20941 gtcctgtgga aaaaatacgg caatggcgac aacggtattc ccggccgata gtgaatgacc
    21001 tgtttgcctg gcctgaagag taggagccgt gctgtccgcc cgacggacca ctaaacaaag
    21061 cgattaatta tatcctgaac cggcgcgatg aactgagctg cttcctgagt gacggagccg
    21121 tgcccctgga caacaatatc tgcgagcgtg ctatccgccc ggtggtcatg gggcggaaag
    21181 catggttatt cgccggttca ctgatggccg ggaaccgcgc ggcacagata atgagccttc
    21241 tggaaacggc aaaacgtaat ggtctggagc cgcatgcctg gctgacagac gttctgacgc
    21301 gtctaccgga gtggccggaa gaccgactgg aagagttgct gccccttgag ggatttacct
    21361 tctccgggtg agtgatacct gccatcaggt gttcgtgcag cgggccataa cccgcagtcg
    21421 ggagttgaac gcctgacgac aggaaatgag ccagagcagc gaccacgcgg gctgacagtg
    21481 agtcagaacc tgaatcagcc cggagccatt cccggggctg tacttcatca cctgccatca
    21541 gtacccgtgt cagtacttcg cgacctgacg caattacccg actcagttct tcagaacccg
    21601 cctccgatac cagcctggcg gtcgccccgg cgcaacagaa taaatcaacc atcgtcagac
    21661 tgcgatcatt cgtatgcatc gctgatgatg cgcccgaaaa acgcgtcaat atgagttcgc
    21721 cggaatatta ctgacgacac ggtttgtagt tacgttcctt cggtgagtga gtacctgtgc
    21781 gtcagggtaa cgcaatcagt tcaggtttag cggggtgtca agtcggtttg gctaaccgac
    21841 cgcagaatta actgtacctt taaaggtcgc ccccgatata tctgcctgtc agacatatcg
    21901 ccgattcggt atctcactac ataaaactcc ttaaaaaaca atgcgattct gttggtacga
    21961 agctttcagt aagtcagtcc ggagaagcgc cagtacgata cagaatgagg ttgaaaaaaa
    22021 cgttcacgtc tggtcacttc ttcaaatgtt aaaaaacatc tcgtaaagag ccagtttatg
    22081 cgggttcgtt gagtagacgt gcgagcgtca ctttgtcagc tttaacgctg acgaaagtga
    22141 cgcccgcaca gcttcaaact ccacatgcac acgcaagcga ccaaatttca aatggtctta
    22201 tgccgtgcgc acagtcataa gaagcgcatg cggaaagtca gaaaatatct taagacgcag
    22261 attttaaagg cgtttttatg ccagtctgcc gggaatacac agtagtccgg ttgtccggaa
    22321 gcaccacggt aatccgaagg aaaaatcaac gtacctcaat caccgtagtg atatccgttc
    22381 ccagaggcta aacggatgta ccaccataaa ggcatcctct ttcaggctac gaggatattg
    22441 caatcacccg aaggcgtttc tctcagattg ctgaaacatt ggctggcagt cacccgaagg
    22501 cgtccctctc agactgctgg aacattggct ggtcgcaaaa gcgacagtta atgcaaatac
    22561 aggttaacaa aaagggaaat ctgccgtcaa cccaaccata tgaacatgtt gatttcatta
    22621 tgaatgtgat gttctaatat aaattaatca gtaacttcat aaaaagaata taagaaacag
    22681 ataatgcatg tatttacaca catatcagca caatgataga ttaccacccc agaaacacga
    22741 taaaggtata attatggaat tttccgttat ttttttctgg gttatcactg gtcttattac
    22801 catcttattc ttaaaatcaa taataaccga tctaataaaa a
//
LOCUS       DAZGCS010000076        22610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGCS010000076 DAZGCS010000000
VERSION     DAZGCS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.076"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..703
                     /locus_tag="ACHK01_003217"
     CDS             32..703
                     /locus_tag="ACHK01_003217"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO8098128.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            713..1909
                     /gene="yaiP"
                     /locus_tag="ACHK01_003218"
     CDS             713..1909
                     /gene="yaiP"
                     /locus_tag="ACHK01_003218"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8098129.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1887..2468
                     /locus_tag="ACHK01_003219"
     CDS             1887..2468
                     /locus_tag="ACHK01_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8098130.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2470..3243
                     /gene="yaiO"
                     /locus_tag="ACHK01_003220"
     CDS             2470..3243
                     /gene="yaiO"
                     /locus_tag="ACHK01_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO8098131.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3428..3703
                     /gene="frmR"
                     /locus_tag="ACHK01_003221"
     CDS             3428..3703
                     /gene="frmR"
                     /locus_tag="ACHK01_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO8098132.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3738..4847
                     /gene="frmA"
                     /locus_tag="ACHK01_003222"
     CDS             3738..4847
                     /gene="frmA"
                     /locus_tag="ACHK01_003222"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO8098133.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4940..5773
                     /gene="fghA"
                     /locus_tag="ACHK01_003223"
     CDS             4940..5773
                     /gene="fghA"
                     /locus_tag="ACHK01_003223"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8098134.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIHYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5898..6437)
                     /gene="yaiL"
                     /locus_tag="ACHK01_003224"
     CDS             complement(5898..6437)
                     /gene="yaiL"
                     /locus_tag="ACHK01_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO8098135.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6539..7750)
                     /gene="mhpT"
                     /locus_tag="ACHK01_003225"
     CDS             complement(6539..7750)
                     /gene="mhpT"
                     /locus_tag="ACHK01_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO8098136.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFSLQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8025..9038)
                     /gene="mhpE"
                     /locus_tag="ACHK01_003226"
     CDS             complement(8025..9038)
                     /gene="mhpE"
                     /locus_tag="ACHK01_003226"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO8098137.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9035..9985)
                     /gene="mhpF"
                     /locus_tag="ACHK01_003227"
     CDS             complement(9035..9985)
                     /gene="mhpF"
                     /locus_tag="ACHK01_003227"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO8098138.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9982..10791)
                     /gene="mhpD"
                     /locus_tag="ACHK01_003228"
     CDS             complement(9982..10791)
                     /gene="mhpD"
                     /locus_tag="ACHK01_003228"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO8098139.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10801..11667)
                     /gene="mhpC"
                     /locus_tag="ACHK01_003229"
     CDS             complement(10801..11667)
                     /gene="mhpC"
                     /locus_tag="ACHK01_003229"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO8098140.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11685..12629)
                     /gene="mhpB"
                     /locus_tag="ACHK01_003230"
     CDS             complement(11685..12629)
                     /gene="mhpB"
                     /locus_tag="ACHK01_003230"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO8098141.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12631..14295)
                     /gene="mhpA"
                     /locus_tag="ACHK01_003231"
     CDS             complement(12631..14295)
                     /gene="mhpA"
                     /locus_tag="ACHK01_003231"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO8098142.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14486..15319
                     /gene="mhpR"
                     /locus_tag="ACHK01_003232"
     CDS             14486..15319
                     /gene="mhpR"
                     /locus_tag="ACHK01_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO8098143.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRPMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15396..16478
                     /gene="lacI"
                     /locus_tag="ACHK01_003233"
     CDS             15396..16478
                     /gene="lacI"
                     /locus_tag="ACHK01_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO8098144.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16601..19675
                     /gene="lacZ"
                     /locus_tag="ACHK01_003234"
     CDS             16601..19675
                     /gene="lacZ"
                     /locus_tag="ACHK01_003234"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO8098145.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWNKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19727..20980
                     /gene="lacY"
                     /locus_tag="ACHK01_003235"
     CDS             19727..20980
                     /gene="lacY"
                     /locus_tag="ACHK01_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO8098146.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21046..21657
                     /gene="lacA"
                     /locus_tag="ACHK01_003236"
     CDS             21046..21657
                     /gene="lacA"
                     /locus_tag="ACHK01_003236"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO8098147.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(21760..>22610)
                     /gene="cynX"
                     /locus_tag="ACHK01_003237"
     CDS             complement(21760..>22610)
                     /gene="cynX"
                     /locus_tag="ACHK01_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO8098148.1"
                     /translation="GIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWL
                     VQHSESWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVY
                     FGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRK
                     LLMLALVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGK
                     LVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQ
                     LWVKEA"
BASE COUNT         5346 a         6136 c         5853 g         5275 t
ORIGIN      
        1 tagtataata aataaaatgg agtgtcatta agtggataag gttttagatt cagccctcct
       61 ttcttcggca aataaaagaa agggtatcct tgctattggc gcacatcctg acgatataga
      121 attaggctgt ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata tcgcagccgt
      181 ggtgatgact accggcaact ctggcacaga tggaataatc gatcgccatg aagaatcgcg
      241 caacgcccta aagatattag ggtgccacca aactattcat cttaattttg ctgacacccg
      301 cgctcattta cagctcaatg atatgatttc cgccctggaa gacatcatta aaaatcaaat
      361 tccttctgat gttgaaatca tgcgggtata taccatgcat gatgccgacc gccatcagga
      421 tcatctcgct gtttatcaag cttcaatggt tgcctgccgc actattccac aaattctcgg
      481 ctacgaaacc ccaagtacct ggctttcatt tatgcctcag gtttttgaat ccgttaaaga
      541 agaatatttc acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg aacgacgcga
      601 ttatatgcgt catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac aggtcaatag
      661 cgatctgggt gaaggctttg taattcataa aatgattctt tgagcaaatt gcatgaaaac
      721 ctggatattt atctgtatgt ccatagcaat gttgctatgg tttttaagta cgctaagacg
      781 taaacccagt caaaagaaag gctgtattga cgccattata cctgcgtata acgaaggccc
      841 gtgtctggcg cagtcactgg ataatctact gcgtaaccct tatttttgcc gggtaatttg
      901 cgttaacgac ggctccacgg acaataccga agcggtcatg gcggaagtca aacgcaaatg
      961 gggcgaccgc tttgttgccg tcacgcaaaa aaataccggt aaaggtggtg cgctgatgaa
     1021 tggcctcaat tatgccacct gcgaccaggt ttttttaagt gatgccgaca cctatgttcc
     1081 gcccgatcaa gacggaatgg gctatatgct ggcagaaatt gagcgcggtg ctgatgccgt
     1141 aggcggcatt ccctctactg cgttgaaagg cgcggggctg ttaccgcaca tccgcgcgac
     1201 cgtaaagttg ccgatgattg ttatgaagcg cacgctacag cagctcctgg gcggcgcacc
     1261 gtttattatc agcggtgcct gcggaatgtt ccgcactgat gtattgcgta agttcggttt
     1321 ctcagatcgt actaaagtcg aagaccttga tctcacctgg acattagtgg caaacggcta
     1381 ccgtattcgg caggcgaatc gctgcatcgt atacccacag gaatgcaaca gcccgcgtga
     1441 ggagtggcgt cgctggcggc gttggattgt gggatacgcg gtctgtatgc gcctgcataa
     1501 aagactttta tttagccgct tcggtatctt cagtatattt cctatgctgt tggttgtgat
     1561 ttatggcgtt gggatttatc tcactacctg gtttaatgaa ttcatcacca ccgggccgca
     1621 tggtgtggtg ttggcaatgt ttccgcttat ctggatcggc gtagtttgtg ttattggtgc
     1681 ttttagcgcc tggtttcatc gttgctggtt gttggtgcct ttagcgccgc tttccgttgt
     1741 gtatgtatta ttagcttatg ccatctggat tatttatgga cttattgcct tttttactgg
     1801 acgcgaacct cagcgcgaca aacccacccg ctattccgca ctggtggaag cgtcaaccgc
     1861 ttattcccaa ccttctgtca caggaactga aaaactatct gaagcttaat gttaaagaga
     1921 aaaatattca gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg
     1981 gcgcgaatac ccgtatttgt catggtgccg ttattcaggg tccggtagtg attggcgcaa
     2041 actgcctgat aggtaattat gcgtttattc gtcctggcac aataatcagc aatggtgtaa
     2101 aaattggttt tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc
     2161 cgcaatgttt tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac
     2221 gtaccagtaa tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta
     2281 tcgctaccgg atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac
     2341 aagttataat tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga
     2401 ttgtagaacg taatttacct gccggaactt attcactccg acaagaactt atccgtacag
     2461 gagattaaaa tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct
     2521 tatgccgggt taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat
     2581 ggcaaccgta atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt
     2641 tttaatcttt cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt
     2701 cagggtgcgg tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt
     2761 gcggataata cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg
     2821 ttgccaaaaa cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa
     2881 gtcgatgcct ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc
     2941 tatacccatt atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt
     3001 ctgaatgacc cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggcgct
     3061 tacacctatg actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtttgcaa
     3121 cgtattcaac cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac
     3181 gacaccccaa ccgatgatta taacggtctg caacttgcag cccatctgac ctggaaattc
     3241 tgattccttc tgccgcccgc tatccggggc ggccttccct gccgattagc cccccctttc
     3301 ctctttgttt tccgaccaca ttcaccggat aaattttatt ctccagtgtt atatactata
     3361 ggggggtatg cattgacata tagcataccc ccctatagta tattgcgtgc agatgatgag
     3421 gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc cttactcgag ttcgtcgtat
     3481 tcgagggcag attgatgctc tggaacggtc gctggagggt gatgccgaat gccgtgccat
     3541 actccaacag atcgctgccg ttcggggcgc ggctaacggg ctgatggcag aagtgcttga
     3601 aagccatatc cgggaaacgt ttgaccgaaa tgactgctac agccgcgaag tcagccaatc
     3661 cgttgacgac actattgaac tggttcgagc ctatcttaaa tagctgaatc tattaccata
     3721 ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc atttgctccc ggtaaaccgc
     3781 tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg tgaagtgctg attaaagtca
     3841 cccataccgg cgtttgccat accgacgcat ttaccctctc cggggatgac ccggaaggtg
     3901 tattcccggt ggttctcggt cacgaagggg ccggcgttgt ggttgaagtc ggtgaaggcg
     3961 taaccagcgt caaacctggc gaccatgtga tcccgcttta caccgcggag tgcggcgagt
     4021 gtgagttctg tcgttctggc aaaactaacc tctgtgttgc ggttcgcgaa acccagggta
     4081 aaggcttgat gccagacggc accacccgtt tttcttacaa cgggcagccg ctttatcact
     4141 acatggggtg ctctacattc agtgaataca ccgtggtcgc ggaagtgtct ctggccaaaa
     4201 ttaatccaga agcaaaccat gaacacgtct gtctgctggg ctgtggcgtg accaccggta
     4261 ttggcgcggt gcacaacaca gctaaagtcc agccaggtga ttctgttgcc gtgtttggtc
     4321 ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca ggcgaaagcg ggacggatta
     4381 tcgctatcga taccaacccg aagaaattcg atctggcgcg tcgcttcggt gctaccgact
     4441 gcattaaccc gaatgactac gacaaaccga ttaaagatgt cctgctggat atcaacaaat
     4501 ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa cgtgatgcgt gcggcgctgg
     4561 aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg ggtggcaggt tctggtcagg
     4621 aaatctccac ccgtccattc cagttggtca ctggtcgcgt atggaaaggt tccgcgtttg
     4681 gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga agatgcgatg aaaggtgata
     4741 tcgatctgga accgtttgtc acgcatacca tgagccttga tgaaattaat gacgccttcg
     4801 acctgatgca tgaaggcaaa tccattcgaa ccgtaattcg ttactgattt cccgcaggta
     4861 taccccgtcc acttcagacg gggttcttaa tactctcccc gggcagccgt ccgggggatt
     4921 aaccgtgaga taatgactga tggaactcat tgaaaaacat gccagctttg gcggctggca
     4981 aaatgtgtat cggcattatt cccaatcact gaaatgtgaa atgaatgtcg gcgtctatct
     5041 cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac tggctttcgg gcctgacctg
     5101 caacgagcag aatttcatta ctaaatcggg gatgcagcgt tacgcggctg aacacaacat
     5161 tattgttgtt gcgccggaca ccagtccgcg aggcagtcat gtcgcagatg ccgaccgtta
     5221 cgatctcggg caaggtgccg ggttttacct gaacgcaacg caagcgccgt ggaatgaaca
     5281 ttacaaaatg tatgactata tccgcaacga gctgccggat ttagtgatgc aacattttcc
     5341 ggcaacgacc agaaagtcta tctctggtca ttctatgggc gggctgggcg cgctggtgct
     5401 ggcgttacgt aacccagatg aatatgtcag cgtctcggcg ttttcgccca ttgtctcccc
     5461 atcgcaagta ccgtggggac agcaagcctt tgccgcatat cttggtgaaa ataaagatgc
     5521 ctggttgaat tacgatccgg tgagtcttat ttcacaaggt caacgcgttg cggaaatcat
     5581 ggttgatcag gggttgagtg atgattttta cgcggaacag ctgcggactc caaatcttga
     5641 aaagatctgc caggagatga atatcaagac gttaatccat tatcacgagg gttacgacca
     5701 cagctattat tttgtctcca gttttattgg cgagcatatt gcctaccacg ccaataaact
     5761 gaatatgcgt tgataatagt gcacgactgc cggatgcggc gtgaacgcct tatccgccta
     5821 catttcgccc gcaggccgta ggcatgataa gacgcgcata gcgtcgcatc aggcatctgc
     5881 gcacgactta tctatgttta ccacatcaaa tcatcaggca ctttgaagtc ggcatatggg
     5941 tcgtcttcat cctgctcttc ggcactgagc gcgctgtgca acacaatact gctggcatca
     6001 cgctgagcaa ttttatcggc aacgctggcg ggaataatgg cgtattcacc ttcgctattg
     6061 ttatcgacca acaagcgggc aatcgccaga cgaccattaa tcaactgcgc ctgtgtgagc
     6121 ttatcgacaa aaatcttctt aatcagattg ccgtcagtga agttaaaacc aatatcacca
     6181 ttggcaatgg tgattcggtt catttcaata agctgcttca cctgggcttt atattctttc
     6241 gccaacgccg cttgtttttg ctgttcgcta agctgtttat cacgctcaag ctgtgccttt
     6301 ttattttctt ctaccgccgc ccgagcttca cgcgcctgaa cgcgcgattt tttcgccgtt
     6361 ctctcgacct tcgccgcttt tttgctggtg actaatcctg ctttgagcaa ctgctcttgt
     6421 aaggtaagtt ttgccatctt tgtttccaaa cccgctgaat aatttttagc gagtatacct
     6481 gtaaacgccg tgggtgtatt gggggcaagt ccctttcgcc cctgctaaat aaggcgcgtc
     6541 aggcatcggc acacggctgc attcttgatc tccgactcat caaaataaac accgccaacc
     6601 cagcaacaag aatacccggt gcagaagcgg ccattacgcc gaccgtgcca gtgcctaatg
     6661 ccagcatttt cccggccagt aacggaccgc tcatagcccc cagacgccct accgccacgg
     6721 ctgttcccac acctgttgcg cggatctgcg aactgtaaaa caacggtgcc agggcataca
     6781 aaacgctttg cccacctgtc gcaaacaacc ccgcgacaaa tcccgccagc aacataccgt
     6841 taaatgacga caccgttcca agcgccagca gcgaagctaa catgccgcta taaatcagta
     6901 gcgacatggt tactggacgc agcttatcca tcaatgcgcc caacattaac gtcccgcttg
     6961 ccgcccccat ttgcagggaa aacatcaccc ctgccgcctg cgatggctgg aatccttgct
     7021 ccaccaaaag tagcggtagc cagttgatca acatataaac caccagcaga gtgaagaaat
     7081 aacacaacca cagcagcagc gtcgcggttg ccgtttctgg cgcaaataag gcacgcagtg
     7141 gtggcgcagc ctgtttttcg ccagcgaaaa ccgctgactc cggcagccag cgcattaata
     7201 gcggcaccag aatcaacggc accacaccac ctacccaaaa caccgtttgc catgctaagt
     7261 ttgcccccgc gaaacccagt gtcgccgcca gcgccgcgcc aatgggaaca ccgcaataca
     7321 tcaggctcac tgccgtccca cgaaaacgtg gacccgcggc ttcagacgtc agggcgataa
     7381 gattcggcaa cgccgccccc agcccgacac cggtcatcag ccgcgcaaag accagtgagg
     7441 ggaaatccca ggcaatcgcc gttgccagtg agaacaaacc aaacagcgca actgagccaa
     7501 tcaaaatact cttacgacca taacggtctg ccagcatccc gccaaccaac gcgccgggta
     7561 gcaaaccgag tattccggcg ctaaatatcc agcccatttg cattttatcg agtgcgaaag
     7621 cctgggcgat gccacccgcc gcaatgccag ccgcctgaag atccagccct tccatcagag
     7681 cgaccaaaaa acaaagcccg atggtcagca tcaggcggga tgaagatgat gaaggggtac
     7741 gagtcgacat aagatgaacc tcgttgcgag tgcaggtgaa tcgcgactga tacattgcca
     7801 gccgcgtaat cagaactatt taatgactgc ctacgggcga aatgtaggcc ggacaagacg
     7861 tttatcgcat ccggcagtcg tgtgcagatg cctgatgcga cgctaacgcg tcttatcagg
     7921 cctacaaatt ctcacgccac ccgtaggccg gataaggtgt ttacgccgca tccggcagtc
     7981 gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc atcattattt gttgttgcgc
     8041 agatccagcg ccacgtcaac gatcatatcc tcctggccgc caaccatccg gcgtttgccc
     8101 agctcaacga gaatatccac cgcacttaag ccataacgcg ccgccgccgt ttcacagtga
     8161 cgcaggaagc tcgagtaaac accagcgtat cccagcgcca gcgtttcgcg atcgactcgt
     8221 accggtcgat cctgcaacgg acgcaccagg tcgtcagcgg catccattaa cgcatagaga
     8281 tcggtcccat gctgccagcc cagtttatcc gcggcggcaa taaacacttc cagcggtgcg
     8341 ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga ttcggtcgca gccctcttcc
     8401 accgccgcga tagagttcgc cacgccaaga ctcaggttat ggtgagcgtg catgccagtt
     8461 tgcgtttctg gtttcagctc tgctttcagg gcgcggaaac ggtcacggat atcgctcatg
     8521 ttcatcgcac cgccagaatc caccacataa atacaggtcg caccgtagcc ttccatcagc
     8581 tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc tcatcatcag aaaaccaacg
     8641 gtgtccattc cgagctcgcg ggcatactga atatgctggg cggaaacatc agcttcggta
     8701 cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc aggcattttt cagatcgtga
     8761 atagtgccga ttcctggcag caacaacgtc gcgattttgg cgtgcttcac cacatccgcc
     8821 gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac catagttaaa gctggaaccc
     8881 tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc gggcatcgtc cagtgctttg
     8941 gcaatctggc gaacgttttc cagcgaatac tgatgacgaa tggcgtgcat accgtcacgc
     9001 aacgtgacgt ccgagatata aagtttttta ccgttcatgc cgcttctcct gccttgcgcg
     9061 ccagtgactg ggccattttt tccgctgtcg ccagcgcact ggaagtcata atgtcgaggt
     9121 tgcccgcata ggcaggcaga taatgcgctg cgccttcgac ttccagccag accgctgttt
     9181 tcagtccgga gaattgcccc acgcccggta aattgaccgg tttatcctgc gggataactt
     9241 caaactgcac gcgctgtttc aggcgataac ccggtacgta agcctgcacc gcctcagcca
     9301 tttcattgat tgaggcttcg atatcatctt gtgaagcttc gtcgctcaat acatacaccg
     9361 tgtcacgcat catcaacggt ggctctgctg ggttaagcac aataatcgcc ttcccttttg
     9421 ccgcgccgcc caccacttca atggctcggg aagtggtttc cgtaaattca tcgatattgg
     9481 cacgcgtgcc aggtccggca gatttactgg cgatagaagc gataatttcg gcgtaatgaa
     9541 cacgcgccac gcgtgaaact gccgccacca ttggaatggt ggcctggccg ccgcaggtga
     9601 ccatgttgac gttcagttga tcgacgttcg cctcgaggtt aaccaccggc acgcagtaag
     9661 ggccgatggc agcaggcgtc aggtcaatta agcgaatatc cggtttcgct tcgcgtaaag
     9721 cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc aaatacaatg tcgatatcag
     9781 caaattcagg catgttcatc agcccgatca ccccttcatg ggtggtggcg acgcccatac
     9841 gtctggcacg cgccagaccg tcggactgag gatcaatgcc gaccatcacc gccatctcca
     9901 gatgctgacc gtgacgcaaa attttaatca tcagatcggt gccaatgttg ccagaaccga
     9961 taatggcgac tttacgctta ctcatgacag acttcctttt ggggctgcgc ttgaaaatgt
    10021 cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa cgatcgcccg cattcactgc
    10081 caccatcgga cctaatgccc cggtaagaat gatatctccg gcgcgcagcg gttcacccag
    10141 gctggccatt ttgcgtgcca gccagacggc ggcattaagc ggatgtccca ggcattcgct
    10201 gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc ttcatggcgc agtttttcag
    10261 gtctaacccc gccggacgtt gcgccggacc gccgatgaca tacaccccgc aggaagcgtt
    10321 atctgccacg gtatcgacaa actgaatcga ccagtcgcga atgcggctac ccaccacctc
    10381 cagcgccgga agtacccatt caatggcgtt atacagttcg tcgaaggtga tatcggttgc
    10441 gggcaaatcg cggttcaaca ccagtgcgat ctccgcttca atgcgggctt gcagaacacg
    10501 ggaaaaagga atgatttcgt tatcgccata acacatgtcg gcaaataatg tcccaaaatc
    10561 cggttgatca acgcccagtt gttgctgcac tttcggatgc gtcaggccca ctttacgccc
    10621 taccacgcga cgcccctgcg caacgtcata ttgcacattt atgtgctgaa tggcgtaggc
    10681 cgcttcagcg ttatcgatac caatcagatc gcgcagcggc gcaatcgctt cgccctgctc
    10741 tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta tgcttcgtca tgaccattcc
    10801 ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa gcgtcggcat gttcccactg
    10861 cgcccagtga ccacagtcgc ggaagatatg cagttcagaa ccggcaatgc cggacagcag
    10921 acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc ccccagacaa tcagggtttg
    10981 cgctttgatt tccgccagac gtgggccaaa atccgggaac tgtttcggat tagcttccag
    11041 gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc atattattca ggcgcgcttc
    11101 aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag atatccatca tcagcttcag
    11161 gttttcgata gtcggctgac gataaagctg attcagtcgc ttaatacctt cggttggcat
    11221 cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc agcaccagtt tgccgacgcg
    11281 ctccggccag ttaagggtga acgccataga actatggccg cccatcgagt tgcccagcag
    11341 gtggattttg gcgatatcca gttgatccac cacgcttttc aggattcgtg cattaagatc
    11401 cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc caacccggac aatccagcag
    11461 gattacccga tagcccgcct ctaccagcgg atcgatattg cggctgaagt tcgcccagcc
    11521 agtagcaccc gggccggaac cgtgcagcag gacaacggtt tcgtcgcctt gtccgcagtc
    11581 attaaaatgg atgcgcagcg ttttacccgc ttcttctaca ttcagaaaac ggctggtggc
    11641 ggcttcggtt tgtggctgat aactcatcat cttctcctgc atattcagtt ctctgttctg
    11701 gcgcttaacg agccaaatcc ggcaatccac tccgggattg ggcggtaata acgcccttcg
    11761 ctacgccagt tgccaaatgc agaaatagcg gcaaaagcgg cgacccaggt tttgatttca
    11821 tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac tgacggcatc cagttcctgt
    11881 atgcgtccct gctccagcaa agtcatgaac tggttatccc aaatcgggtt gagcggatgc
    11941 agagttctct gatcctcaac aaacttctca gcggcgctaa tcacccgttg ctgacgcaat
    12001 tcgcgctcac tggcgggtaa atctttcccg ctccccaaca gacggtcgcg catatgggca
    12061 tcggctttcg ccagttcggg caccggcggc tgatgggaaa gcccaccgga acccaggaac
    12121 agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg cttcacccaa catgcgggta
    12181 cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga acacaggcag aactggcacc
    12241 ttatccagcc cgccgagcag gaactccagt ggctgggcga acccgtggtc cacctgcata
    12301 cagtaagaaa cggcaagatc gatcccgctc ttcatgacgg catgcgcaca ggcctccgcc
    12361 agctccacag gcactggcag ctctcctgcc gcactgccga aatcaccaat tgccgtcgct
    12421 ccaacgccta aacagaacgg tggcatcacg tcatagaaaa agccgttgta gtgatctggc
    12481 gcaaacagca ccaccagttc aggggagaaa gccgcaatac gctcgcgggc gctggcaatc
    12541 acgccattga cctcatcgag cacctcttgc gccgggtcga cataccccac cagcggcgag
    12601 tgggaaagac agtgaagata agcgtgcata tcaggctacc ttttcgacag aaacgtcggc
    12661 atcagggcgg gtcagcgtca tcaccgacgc cagtttattc agggtcttgc ccagggtttg
    12721 cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc accaccagcg aagcattgtg
    12781 ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc acgcgtagca cgccgtcgtg
    12841 gttatcctgt gcggtatgaa tttgcacttc cggcaccacc tgaatgaagc gtgtgcccaa
    12901 cgcgcgccac tgctggattt gctcgtcgct catcccccac agtggattgc atccccagcc
    12961 aattaccgcg aagttcgcgc caatcgcgtt atcgagcagc gtcacgtcac cgttttccag
    13021 cgtgactttc ggctgaataa acatcttgcc gaccggagag tgcttcgctt cgccctcacg
    13081 caccagcgca ccgccgtaat attgcggcat cggcttgaag cgcatttcga ggaaatagcg
    13141 ttttactggc ggcagataat tcaacagcca ggaaacgccg tcacgtaacg taccctgcca
    13201 gcgtttcggc ggagccagca cgttgcccgc cgtcacggac aggtcaatca tcgctttggc
    13261 gtgatcgcga cgttcttgtt gataggtatc gagcagcgca tcgcgggctt tcccctggat
    13321 aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc ataccactgt tatagccctg
    13381 cccctgccac accggcatga tgtgcgcggc atcgcccgcc agcagtacgc gatcaatacg
    13441 gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc tggcgaatca attcaacatt
    13501 gtccggatta ggcagcactt tgcttaacag cttgcgcata ttttgcggct cacgcagctg
    13561 ctcttcggtt tctcccggca tcaccataaa ttcaaagcga cgtaccgcat gaggcagcgc
    13621 ggcagaaaca tacgggcgca ccggatcgca acacaaatag atatgcggcg tacttaacgg
    13681 atcgttggcg atatctacca caatccactg atttggcgca gttttacctt caaacggcac
    13741 attcagagtg cgacggacaa aacttgctcc accgtcacag gctaccagcc actgggcttt
    13801 gaccgtttcc cgctgccctt ctgccgtttt caggtgcaag gtcacttcgt cattttgctg
    13861 gctgaaggcc tccagctcgc gggaaaacaa gcagcgcaca ttcggaaaac gcgacaaccc
    13921 ttccagcatc accgcatcga cctgaggctg aataaaggcg ttacggcgcg gccagccaaa
    13981 ttcatcggtc attggctgaa tatcagcaaa acagcggcct ttcggggtga gaaaacgcat
    14041 cgcgtgccac ggcgtagtgt gcggcagaac atcatcgacc aggccgaccg actgcatggt
    14101 gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag tcgatcaact tatcgagttt
    14161 ttccaccacc agcacgtcaa tgcccatctg gccgagatag ttcgccatca tcagcccgac
    14221 cggaccggca ccagcgatcg ccacctgaac gctatggtta acagcaggct ggatgtcagg
    14281 gtgttgtatt gccatttcag tacctcacga ctcggacaaa atgccgttgc gcgcacagta
    14341 cggcgcaact tattttgtta aaaacatgta aatgattttt tattgtgcgc tcagtatagg
    14401 aagggtgttt tcggctacaa tcaaaacatg cccgaatgtg caccaggtgc acaactttgt
    14461 tttaactata gaaatgtcaa ttaatatgca gaacaatgag cagacggaat acaaaaccgt
    14521 gcgcggctta acccgcggtc taatgttatt aaatatgtta aataaacttg atggcggtgc
    14581 cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc accactgtgc ggcgactgct
    14641 ggagacgctg caggaagagg gatatgtccg ccgtagcccc tccgatgata gttttcgtct
    14701 gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac gaacagtgga tttctgcact
    14761 ggcggcccca ctgctgggcg atctgttgcg cgaagtggta tggccgacag atgtgtccac
    14821 gctggatgtt gatgcaatgg tggtacgcga aaccactcac cgtttcagcc gcttatcctt
    14881 tcaccggcca atggtcgggc gacgtttgcc gcttctgaaa accgcctctg gcctgacctg
    14941 gctggccttt tgcccggaac aagaccgcaa ggaattaatc gaaatgttag cctcccgccc
    15001 cggtgatgac tatcaactgg cacgggaacc gttaaagctg gaagccattc tggcgcgcgc
    15061 gcgcaaagag ggttacggac agaactaccg cggctgggat caggaggaga agatcgcctc
    15121 tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat
    15181 ggcgagcgca atgaccattg aacaggcagc ggaaaagcat cttccggcgc tacaacgggt
    15241 agcaaaacag atcgaagaag gggttgaatc gcaggctatt ctggtggccg gacggcgaag
    15301 cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat
    15361 agcgtccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg
    15421 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
    15481 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
    15541 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
    15601 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgctgatc
    15661 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
    15721 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
    15781 atgaccagga tgccactgct gtggaagcag cctgcgctaa tgttccggcg ttatttcttg
    15841 atgtctctga ccagactccc atcaacagta ttattttctc ccatgaagac ggtacgcggc
    15901 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
    15961 taagttctgt ctcggcgcgt ctgcgtctgg cgggctggca taaatatctc acacgcaatc
    16021 aaattcagcc gatagcggaa cgggagggcg actggagtgc catgtccggt tttcaacaaa
    16081 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
    16141 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
    16201 cggtagtggg atacgacgat accgaagaca gctcgtgtta tatcccgccg ttaaccacca
    16261 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
    16321 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
    16381 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    16441 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    16501 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    16561 gaaattgtga gcggataaca attttacaca ggatacagct atgactatga ttacggattc
    16621 actggtcgtc gtattacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
    16681 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
    16741 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
    16801 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
    16861 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc
    16921 cattacggtc aatccgcctt ttgttcccgc ggagaatccg acgggttgtt actcgctcac
    16981 atttaatatt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggtgt
    17041 taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagccg
    17101 tttgccgtct gaatttgacc tgagcacatt tttacgcgcc ggagaaaacc gcctcgcggt
    17161 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
    17221 cggcattttc cgtgacgtct cgttgctgca caaaccgagc acacaaatca gcgatttcca
    17281 tgttgccact cactttaatg atgatttcag ccgcgcggta ctggaagcag aagttcagat
    17341 gtatggcgag ctgcgcgatg agctgcgggt gacggtttct ttgtggcagg gtgaaacgca
    17401 ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
    17461 cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc
    17521 gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
    17581 agccagcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
    17641 caagccgttg ctgattcgcg gcgttaaccg tcacgagcat catcctctgc atggtcaggt
    17701 catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
    17761 cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
    17821 cctgtatgtg gtagatgaag ccaacattga aacccacggc atggtgccaa tgaatcgtct
    17881 gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
    17941 cgatcgtaat cacccgagtg tgatcatctg gtcgctggga aatgagtcag gccacggcgc
    18001 taatcacgac gcactctatc gctggaacaa atctgtcgat ccatcccgcc cggtgcagta
    18061 tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
    18121 ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
    18181 acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct
    18241 tggcggcttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
    18301 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
    18361 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
    18421 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt
    18481 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
    18541 tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
    18601 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
    18661 gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
    18721 cgcatggtca gaagccggac acatcagcgc ctggcagcag tggcgtctgg cggcaaacct
    18781 cagcgtgaca ctcccctccg cgccccacgc catcccgcaa ctgaccacca gcgaaacgga
    18841 tttttgcatc gagctggata ataagcgttg gcaatttaac cgccagtcag gctttctttc
    18901 acagatgtgg attggcgatg aaaaacaact gctgaccccg ctgcgcgatc agttcatccg
    18961 tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgtattgacc cgaacgcctg
    19021 ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcggcgttgt tgcagtgcac
    19081 ggcagataca cttgccgacg cggtgctgat taccactgtc cacgcgtggc agcatcaggg
    19141 gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
    19201 taccgttgat gttgaagtag cgagcgatac accgcatccg gcgcggattg gcctgacctg
    19261 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
    19321 tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
    19381 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
    19441 tggctcacac cagtggcgcg gcgacttcca gttcaatatc agtcgctaca gccaacaaca
    19501 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
    19561 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
    19621 attccagctg agcgccggtc gctaccatta ccaattggtc tggtgtcaaa aataataata
    19681 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa
    19741 aaacacaaac ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggagc
    19801 ctacttcccg tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac
    19861 gggtattatt tttgccgcta tttctctgtt ctcgctatta ttccaaccgc tgtttggtct
    19921 gctttctgac aaactcgggc tgcgcaaata cctgctgtgg attattaccg gcatgttagt
    19981 gatgtttgcg ccgttcttta tttttatctt cgggccactg ttacaataca acattttagt
    20041 aggatcgatt gttggtggta tttatctagg cttttgtttt aacgccggtg cgccagcagt
    20101 agaggcattt attgagaaag tcagccgtcg cagtaatttc gaatttggtc gcgcgcggat
    20161 gtttggctgt gttggctggg cgctgtgtgc ctcgattgtc ggcatcatgt tcaccatcaa
    20221 taatcagttt gttttctggc tgggttctgg ctgtgcactc atcctcgccg ttttactctt
    20281 tttcgccaaa acggatgcgc cctcttccgc cacggttgcc aatgcggtag gtgccaacca
    20341 ttcggcattt agccttaagc tggcgctgga actgttcaga cagccaaaac tgtggttttt
    20401 gtcactgtat gttattggcg tttcctgcac ctacgatgtt tttgaccaac agtttgctaa
    20461 tttctttact tcgttctttg ctaccggtga acagggtacg cgggtatttg gctacgtaac
    20521 gacaatgggc gaattactta acgcctcgat tatgttcttt gcgccactga tcattaatcg
    20581 catcggtggg aaaaacgccc tgctgctggc tggcactatt atgtctgtac gtattattgg
    20641 ctcatcgttc gccacctcag cgctggaagt ggttattctg aaaacgctgc atatgtttga
    20701 agtaccgttc ctgctggtgg gctgctttaa atatattacc agccagtttg aagtgcgttt
    20761 ttcagcgacg atttatctgg tctgtttctg cttctttaag caactggcga tgatttttat
    20821 gtctgtactg gcgggtaata tgtatgaaag catcggtttc cagggcgctt atctggtgct
    20881 gggtctggtg gcgctgggct tcaccttaat ttccgtgttc acgcttagcg gccccggccc
    20941 gctttctcta ctgcgtcgtc aggtgaatga agtcgcttaa gcaatcaatg tcggatgcgg
    21001 cgcgagcgcc ttatccgacc aacatatcag gacggaatga tcgcaatgaa catgtcgatg
    21061 acagaaagaa taaaagcagg caagctattt accgatatgt gcgaaggctt accggaaaaa
    21121 agacttcgtg ggaaaacgtt aatgtatgag tttaatcact cgcatccatc agaagttgaa
    21181 aaaagagaaa gcctgattaa agaaatgttt gccacggtag gggaaaacgc ctggatagaa
    21241 ccacctgtct atttctctta cggttccaac atccatatag gccgcaattt ttatgcaaat
    21301 ttcaatttaa ccattgtcga cgactacacg gtaacgatcg gtgataacgt attgattgca
    21361 cccaatgtta ctctttccgt tacgggacac cctgtacacc atgaattgag aaaaaacggt
    21421 gagatgtact cttttccgat aacgattggc aataacgtct ggatcggaag tcatgtggtt
    21481 attaatccag gcgtcaccat cggggataat tctgttattg gcgcgggtag tgtcgtcata
    21541 aaagacattc caccaaacgt cgtggctgct ggcgttcctt gccgggttat tcgcgaaata
    21601 aacgaccggg ataagcaata ttatttcaga gattacaaag ttgaatcgtc agtttaaatt
    21661 ataaaaattg cctgatacgc ttcgcttatc aggcctacaa attcagtgat ctacgtaggc
    21721 cgcatccggc gtgaacaaag cgcagaaaca agagtcgcat catgcctctt tgacccacag
    21781 ctgcggaaaa cgtgctggtg caaaacgcag ggttataatc atcagcccaa cgacgcacag
    21841 cgcatgaaat gcccagtcca tcaggtaatt gccgctgata ctacgcagca cgccagaaaa
    21901 ccacggggca agcccggcga tgataaaacc gattccctgc ataaacgcca ccagcttgcc
    21961 agcaatagcc ggttgcacag agtgatcgag cgccagcagc aaacagagcg gaaacgcgcc
    22021 gcccagacct aacccacaca ccatcgccca caataccggc aattgcagcg gcaaccagat
    22081 aaagccgcag aaccccacca gttgtaacac cagcgccagc attaacagtt tgcgccgatc
    22141 ctgatggcga gccatagcag gcatcagcaa agctcctgcg gcttgcccaa gcgtcatcaa
    22201 tgccagtaag gaaccgctgt actgcgcgct ggcaccaatc tcaatataga aagcgggtaa
    22261 ccaggcaatc aggctggcgt aaccgccgtt aatcagaccg aagtaaacac ccagcgtcca
    22321 cgcgcgggga gtgaatacca cgcgaaccgg agtggttgtt gtcttgtggg aagaggcgac
    22381 ctcgcgggcg ctttgccacc accaggcaaa gagcgcaaca acggcaggca gcgcccacca
    22441 ggcgagtgtt tgataccagg attcgctatg ttgaactaac cagggcgtta tggcggcacc
    22501 aagtccaccg ccgcccatca gagccgcaga ccacagcccc atcaccagtg gcgtgcgctg
    22561 ctgaaaccgc cgtttaatca ccgaaggcat caccgcctga atgatgccga
//
LOCUS       DAZGCS010000077        22394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGCS010000077 DAZGCS010000000
VERSION     DAZGCS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.077"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..827
                     /locus_tag="ACHK01_003238"
     CDS             144..827
                     /locus_tag="ACHK01_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8098149.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            871..5721
                     /gene="ehaG"
                     /locus_tag="ACHK01_003239"
     CDS             871..5721
                     /gene="ehaG"
                     /locus_tag="ACHK01_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO8098150.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6089..7744
                     /gene="lldP"
                     /locus_tag="ACHK01_003240"
     CDS             6089..7744
                     /gene="lldP"
                     /locus_tag="ACHK01_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8098151.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7744..8520
                     /gene="lldR"
                     /locus_tag="ACHK01_003241"
     CDS             7744..8520
                     /gene="lldR"
                     /locus_tag="ACHK01_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO8098152.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFSGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8517..9707
                     /gene="lldD"
                     /locus_tag="ACHK01_003242"
     CDS             8517..9707
                     /gene="lldD"
                     /locus_tag="ACHK01_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO8098153.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9893..10366
                     /gene="trmL"
                     /locus_tag="ACHK01_003243"
     CDS             9893..10366
                     /gene="trmL"
                     /locus_tag="ACHK01_003243"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO8098154.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="ACHK01_003244"
     CDS             complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="ACHK01_003244"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO8098155.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="ACHK01_003245"
     CDS             complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="ACHK01_003245"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO8098156.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12339..12806)
                     /gene="secB"
                     /locus_tag="ACHK01_003246"
     CDS             complement(12339..12806)
                     /gene="secB"
                     /locus_tag="ACHK01_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO8098157.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="ACHK01_003247"
     CDS             complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="ACHK01_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO8098158.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13262..13693)
                     /gene="yibN"
                     /locus_tag="ACHK01_003248"
     CDS             complement(13262..13693)
                     /gene="yibN"
                     /locus_tag="ACHK01_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO8098159.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13938..15482
                     /gene="gpmM"
                     /locus_tag="ACHK01_003249"
                     /gene_synonym="pgmI"
     CDS             13938..15482
                     /gene="gpmM"
                     /locus_tag="ACHK01_003249"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO8098160.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15516..16775
                     /gene="envC"
                     /locus_tag="ACHK01_003250"
     CDS             15516..16775
                     /gene="envC"
                     /locus_tag="ACHK01_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO8098161.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16779..17738
                     /gene="yibQ"
                     /locus_tag="ACHK01_003251"
     CDS             16779..17738
                     /gene="yibQ"
                     /locus_tag="ACHK01_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO8098162.1"
                     /translation="MFPFRRNVLAFATLLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17725..18759)
                     /gene="waaH"
                     /locus_tag="ACHK01_003252"
     CDS             complement(17725..18759)
                     /gene="waaH"
                     /locus_tag="ACHK01_003252"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO8098163.1"
                     /translation="MMNSTSKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18998..20023)
                     /gene="tdh"
                     /locus_tag="ACHK01_003253"
     CDS             complement(18998..20023)
                     /gene="tdh"
                     /locus_tag="ACHK01_003253"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO8098164.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20033..21229)
                     /gene="kbl"
                     /locus_tag="ACHK01_003254"
     CDS             complement(20033..21229)
                     /gene="kbl"
                     /locus_tag="ACHK01_003254"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO8098165.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21504..22361)
                     /gene="yibB"
                     /locus_tag="ACHK01_003255"
     CDS             complement(21504..22361)
                     /gene="yibB"
                     /locus_tag="ACHK01_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO8098166.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5721 a         5761 c         5657 g         5255 t
ORIGIN      
        1 atacattttt atgatgaatg cttaaaacaa tatttaatcg acacacatca ctgtcattaa
       61 tgtaaagcaa catagctcac caataataca atggacaact ctaccggcat tgatcgctgg
      121 tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt gctgtcactg
      181 tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag ccagaaagta
      241 gtttactgaa acaacaagaa gaacataata ataaaattgt gctacttgaa aaacaacaag
      301 cacagctaaa gagtcaactc gaaactattc aaaaacaaca aactggcata ataaacagta
      361 caaagacttt aactcacgta attaaatccg ttaaagacca acaaaatact tttattttta
      421 ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caacggttcg gtggctttag
      481 cgggtcgagt gttatccatt gacgccacag agaatggtag tgttattcat atctcactgg
      541 tcaacttatt aagtacacct atctcaaaca ttggttttaa tgcgacatgg ggtggtgaaa
      601 aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga
      661 aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg
      721 tatcgcccaa taacctggga tatctgaagt tagccatcaa catggaaaat attcagttcg
      781 acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac
      841 gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac
      901 cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct
      961 gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg
     1021 gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag tggatcagct
     1081 ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt
     1141 ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc
     1201 gggtcaaaaa cccatgcgat tggcggtgca tcaatggcct ttggggttag tgcaatatca
     1261 gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg
     1321 gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc
     1381 aaagcggaag gagcaaacgc cattgccctg ggaaatgcca ctaaagctac tgagattatg
     1441 agtattgctc ttggcgacac cgccaatgcg tcaaaagcgt attcaatggc gctgggagca
     1501 agtagcgtcg catctgaaga aaacgcaatt gccctggggc gtagcagtgt agctagcggt
     1561 actgacagcc tcgcatttgg cagacaatca cttgccagcg cagcgaacgc tattgcgata
     1621 ggtgctgaga ccgaagccgc tgaaaatgca actgctattg gcaataatgc gaaggcaaaa
     1681 gggactaata gcatggcaat ggggttcgga agccttgccg ataaagtcaa tactatcgca
     1741 ttaggaaatg gcagccaggc tctggcagat aatgcaatcg ccataggcca gggcaacaaa
     1801 gctgatggcg tggatgccat cgctctgggt aatggtagcc agtcgagagg cttaaacacc
     1861 attgccttag gcacagccag taatgcaact ggtgataaga gtcttgcgct tggtagtaat
     1921 agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag attccattgc ggatttagac
     1981 aataccgtct ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt gaaaaatggc
     2041 gcgatcaagt ctgacagtta cgatgccatt aatggttcac agctttatgc cattagcgac
     2101 tcggtagcaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca
     2161 gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta
     2221 cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt
     2281 actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc
     2341 agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac
     2401 accgccaata tcgctactaa taccagcaac attgccacga atacggcaaa tattgccacc
     2461 aataccacca atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg
     2521 ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa
     2581 atcaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct
     2641 cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact
     2701 tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt gactaatctt
     2761 ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc
     2821 gagaccacca gcaaaatcac caacgttaaa gatggcgacc tgacgactgg cagcaccgat
     2881 gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat
     2941 atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag
     3001 gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc
     3061 gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt
     3121 aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct
     3181 tctgtgaata ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat
     3241 tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat
     3301 gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac
     3361 gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta
     3421 aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc
     3481 gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac
     3541 gataatgtcg gtgatgccct gaatgctatc gataccactc ttgacgacgc tctgctctgg
     3601 gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc
     3661 agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac
     3721 ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa
     3781 gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac
     3841 aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga tgagactgcc
     3901 aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat catcactaat
     3961 gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat
     4021 gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc
     4081 gacgcaacct atatccaaga aaacggtgcg ggtattaact atgtgcgtac taacgacgac
     4141 ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg ctacagctat aggttataac
     4201 tctgtcgcca aaggcgatag cagcgtagct attggtcagg gcagctacag cgacgttgat
     4261 acgggtatcg ccctgggtag cagctctgtt tccagccgag tgattgccaa aggctcccgt
     4321 gacaccagca taacggaaaa tggcgttgtt attggttacg acaccacgga tggcgaactg
     4381 ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc aaatcatcaa cgtagccgat
     4441 ggttccgaag cccatgacgc cgttacggtt cgtcaattgc agaatgcgat tggtgcggtc
     4501 gcaaccacgc cgactaaata cttccacgct aattcaacgg aagaagattc actggcagtg
     4561 ggaactgact cgctggcaat gggtgcgaaa accatcgtga atggcgataa aggtattggt
     4621 atcggttatg gtgcctacgt ggacgcgaat gcacttaacg gcattgccat tggtagcaat
     4681 gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg gttctacgac cactcgtggc
     4741 gctcaaacca attataccgc ctacaacatg gacgcaccgc agaactctgt cggtgaattc
     4801 tcagtcggta gtgcggatgg tcaacgtcag atcactaacg tcgcagcagg ttcggctgat
     4861 accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg cgcaggtttc ccagaatacc
     4921 cagagcatta ctaacctgga taatcgggta acgaatcttg attcacgcgt caccaatatc
     4981 gaaaacggta ttggcgatat cgtcaccacc ggtagcacca agtacttcaa gaccaatacc
     5041 gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg cgattggttc tggctccatt
     5101 gctgccgcgg ataacagcgt cgcactgggt acagggtctg tggcaaccga agaaaatacg
     5161 atctctgtag gctcatctac taatcaacgt cgtatcacca acgtagccgc aggtaaaaac
     5221 gacaccgatg ctgttaacgt ggcacagttg aagtcttccg aagctggcgg tgtgcgttac
     5281 gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg cggcaacggc
     5341 ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaattac
     5401 gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg aatggttgag
     5461 atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg
     5521 gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc
     5581 ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt
     5641 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc
     5701 ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa
     5761 ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg
     5821 caattctctg atgaggattg cccttttctt taccagacat ctccccccac aagaattggc
     5881 cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc
     5941 aatattgcca atacataaca tttagttaac cattcattgt cattatccct acacaacaca
     6001 attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac
     6061 gatgtgcgtg gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
     6121 cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc
     6181 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct
     6241 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta
     6301 tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta
     6361 taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac
     6421 ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
     6481 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt
     6541 taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg
     6601 cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat
     6661 tggccagatg gtggggcggc agctaccgtt tatgaccatt atcgtgctgt tctggatcat
     6721 ggcgattatg gacggctggc gcggtatcaa ggagacgtgg cctgcggtcg tggttgcggg
     6781 cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga
     6841 cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcagcc
     6901 agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag gttgatatga cgctggccca
     6961 caccggttac actgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgacagc
     7021 taccgtaaca ctgtggagta tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct
     7081 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc
     7141 accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc
     7201 caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc
     7261 gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctgc ccatatactc
     7321 catcggtatg gtgctggcat tcgcctttat ttcgaactat tccggactgt catcaacact
     7381 ggcgctggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
     7441 gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca
     7501 agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac
     7561 cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
     7621 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
     7681 cacctgtata gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc
     7741 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
     7801 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg
     7861 caactcggcg tatcacgtaa ttcactgcgc gaggcgctgg caaaactggt gagtgaaggc
     7921 gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
     7981 gagcaaaaca tcgtccagcc gctaaaaaca ctgatggccg atgatccgga ttacagtttc
     8041 gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc
     8101 gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
     8161 ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
     8221 aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag
     8281 catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat
     8341 caggctgtca ttgacgccat tttttccggt gatgctgacg gggcgcgtaa agcaatgatg
     8401 gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac
     8461 gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga
     8521 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
     8581 tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt
     8641 tgtcagaagt ggcgctgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa
     8701 cgacgctgtt taatgagaaa ttgtcgatgc cggtggcgct aggtccggta ggtttgtgtg
     8761 gcatgtatgc gcgacgcggc gaagttcagg cagccaaagc ggcggatgcg catggcattc
     8821 cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc
     8881 gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg
     8941 agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg
     9001 gagcgcgtta tcgtgatgcg cattctggaa tgagcggccc gaacgcggca atgcgccgct
     9061 atttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccgc
     9121 atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg
     9181 gctggctggg aaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
     9241 atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg
     9301 cagtacgttt tggtgctgat gggattgtgg tttctaacca cggtggccgc cagctggacg
     9361 gtgtactctc ttccgctcgt gcactgcctg ctattgcaga tgcggtgaaa ggtgatatcg
     9421 ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
     9481 gtgccgacac cgtactgctg ggtcgtgctt tcttgtatgc gctggcaaca gcgggccagg
     9541 cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga
     9601 ctggcgcgaa atcgatcaat gaaattacgc aggattcgct ggtgcagggg ctgggtaaag
     9661 agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgagcccc
     9721 ctcaccctaa ccctctccct cagggagtgg ggaccgttcg gcgctgtatg tactccctca
     9781 ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggtga ggggaaaagg
     9841 ccgcactgct caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa
     9901 catcgtactt tacgaaccag aaattccgcc aaatactggc aatatcatcc gtctttgcgc
     9961 taataccggc tttcgtctgc atatcatcga accgatggga tttgcctggg acgataagcg
    10021 cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgactt
    10081 tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac
    10141 gcctgctcac agcgccgtaa actatcagga tggcgattat ctgatgttcg gcccggaaac
    10201 acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc
    10261 gatggtgccg gacagccgca gcatgaacct gtccaatgcg gtgtcagttg tggtgtatga
    10321 agcctggagg cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg
    10381 atagatgatt acatcgcatc cggcacgatc acaggacatc agatcccatc cccatactca
    10441 aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct
    10501 ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc
    10561 agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt
    10621 ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat
    10681 ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc
    10741 gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct
    10801 gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat
    10861 gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa
    10921 cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc
    10981 gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttcc
    11041 accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg
    11101 tagctcagtg cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc
    11161 agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt
    11221 tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg
    11281 cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtggctgc tgcgctcgtc
    11341 cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa
    11401 tacttgataa atttcctcgg ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg
    11461 gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg
    11521 tacatccatg ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt
    11581 acaggtaagc accagatcgc caagccccgc catgcccata aaggtggcag gatcggcacc
    11641 cagcgccgca ccaagacgcg acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc
    11701 attcgcacca aaaccgatac cgtcggacat ccccgcgcca atggcaatga cgtttttcac
    11761 cgcaccgcca agctgcacac cgatgaaatc cgggttgctg taaacgcgga aacttttgcc
    11821 acagtgcagc aattgttgga gatcatcggc aaaggtctgg tcggtagagg ccaacgaaat
    11881 agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag
    11941 cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc
    12001 ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg
    12061 gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac ggctggctgc
    12121 cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag
    12181 aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca
    12241 gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc
    12301 ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca
    12361 gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc
    12421 gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca ctcacgagca
    12481 tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg
    12541 atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg
    12601 cccagggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg
    12661 gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc
    12721 ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc
    12781 atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac
    12841 tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg
    12901 catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg
    12961 gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga
    13021 tcggcagctc ctggaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat
    13081 acgggcaggt ttctttggta tagatttcaa cattggccat aaattagctc ctgttttttt
    13141 accctgaaat ttcatgttgc agggcggcag caaaatggag tgtccccaga agcttataac
    13201 aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa
    13261 attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca
    13321 gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca
    13381 tgccagaacc gtctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat
    13441 tgttggcttt gatttcactc ggcaacagat tgatagaacc tgcgatatgg cctttacgga
    13501 agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag
    13561 cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa
    13621 ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca
    13681 taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga
    13741 gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg
    13801 gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg
    13861 atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg
    13921 agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg
    13981 gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa
    14041 tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg
    14101 gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc
    14161 gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg
    14221 ctaatccagt gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca
    14281 ttatgggtct gctctccgta ggcggcgtac acagccacga agatcacatc atggcgatgg
    14341 tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc
    14401 gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg
    14461 cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata
    14521 accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagttcc
    14581 aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt
    14641 tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg
    14701 atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg
    14761 tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc
    14821 tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta
    14881 acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg
    14941 aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg
    15001 aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga
    15061 gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca
    15121 ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcag
    15181 ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg
    15241 tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg
    15301 caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt tatgttggtg
    15361 ataagaacgt gaaagcggtt gaaggcggca aactttctga catcgcgccg accatgttgt
    15421 cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat
    15481 aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca
    15541 ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg
    15601 ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg
    15661 caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga
    15721 aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc
    15781 tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc
    15841 aaaaagccgc ccaggagcgc agcctcgccg cgcaactgga tgccgcattc cgtcagggcg
    15901 agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac
    15961 aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa
    16021 cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga gaaacaaagc gagcaacaaa
    16081 cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggcgctg aacgagcgta
    16141 aaaagacgct ggcagggctg gagtcttcca tccagcaagg tcagcaacag ttgagcgagc
    16201 tgcgcgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag
    16261 cgcgtgcaga acgagaagca cgtgaggccc aggcggttcg cgaccgccag aaagaagcga
    16321 cgcgtaaagg caccacctac aaaccgactg aaagcgaaaa atcgctgatg tcccgtaccg
    16381 gtggtctggg cgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc
    16441 gctatggcga acagctacag ggtgaattac gctggaaagg gatggtgatt ggtgcctctg
    16501 aaggtactga agttaaagcg attgccgacg gtcgggtgat tctggctgac tggctgcaag
    16561 gctacggtct ggtggtggtg gttgagcatg gtaaaggcga catgagtctt tacggctata
    16621 atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg
    16681 tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc
    16741 aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg
    16801 ttcttgcatt tgccactctg ttggcgctct cctcccccgt acttgctggc aaacttgcca
    16861 tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt
    16921 ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag
    16981 cgcataacag cggtcacgaa gtgttgattc atctgccaat ggcaccgttg agtaaacagc
    17041 cgctggagaa aaatacgctg cgcccggaga tgagcagcga cgaaattgag cgcattattc
    17101 gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg ggtagcaaga
    17161 tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt
    17221 acttcctcga cagtgtaacc attggtaata cccaggcgat gcgcgccgcg caaggcactg
    17281 gcgtgaaggt tattaaacgg aaggtgttcc tcgacgattc acagaacgaa gcggacatcc
    17341 gtgtgcagtt caatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg
    17401 ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag
    17461 acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac
    17521 cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca
    17581 aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa
    17641 gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac
    17701 agcccgaagc ggcgaagttt aacgctagcg caaattaaag gcgctacgcg taatgcgatg
    17761 gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctggta
    17821 acccaccttc acgctgcgca cattggtaat caggcgctta tacataccgg aagtaaatat
    17881 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcgcaccg catggcaaac
    17941 acgcaatgct tcgtaagtta tttgctgatg aaattccgga taaatcgtaa ttttgtcggc
    18001 ataatttcga tttaatttct ccagcaggcg ggtaatctta atatagtgac gttgataatt
    18061 aaggttttta ttcccttgtc tatgcaaccg actcactgac gtattatgca gataatattt
    18121 atataatgat tgctcggtat atcgcgcacg cagcgcatta aacatgaatt ctgttgtcca
    18181 gacaatatcc tgatgatgta atccggcaat aaatttaatg ttatttttaa caataacatc
    18241 gcggcgataa acccccatcc agacaacgtg agtccaacgg cgcgaagaaa gccccatccg
    18301 cagccagtcc gggccggtta atacaccggt tgagcgaagg cgatcgctgg ggatggattg
    18361 ccaggtttct cccgtttcac gaaaacacca gtcagcgttg cactgcgcca cgtcgaggtc
    18421 gtcctctaac gccatggtca tcagcgtttc gtacatggtg ggatagacct catcgtcagc
    18481 atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc ccacgattac gcgctaccga
    18541 tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga tagttttctg cgtaatgctt
    18601 tgctatttca acagaattat ccgttgaacc atcgttaata ataatgattt ccagagcagt
    18661 ccaggtttgc gtaattaaag attccataca agtgcggaaa tcatcgcccg cattatataa
    18721 cggaataata acactaagtt tactggtgct gttcatcata aattaccgac gacctctgaa
    18781 gttatcctga cgttttacag gctttgtatt aagaaactat taagcctgtg agatattatt
    18841 tttacaggtg agagagtcac ttcagggtag agagataatg agaacagcta tataagagat
    18901 cagcgcaatt acgaactctt tattacactg acaggatttt acatatttaa cggattatcc
    18961 tcaggcaaaa gggctggaat accagccctt gttcgtgtta atcccagctc agaattactt
    19021 tcccggactg gcccgaacgc atagcgtcaa agcccttctg gaaatcatca atagagaaac
    19081 gatgggtaat gatcggcgag agatcgaggc cagactgaat cagcgccgcc atcttgtacc
    19141 aggtttcaaa catctcacga ccgtaaatac ctttaatgaa caagccttta aagatcactt
    19201 tggtccagtc gatagacata tcagacggcg gaatacccag catcgcaata cggccgccgt
    19261 ggttcatggt gtcaagcatg gtacgaaacg ctggcggcgc accggacatt tccagaccga
    19321 catcaaagcc ttcggtcatg cccagttctg ccatcacatc attaaggttt tctttcgcga
    19381 cgttaaccgc acgggtgata cccattttgc gcgccagctc aaggcggtat tcgttaacat
    19441 cggtgatcac cacattgcgt gcaccaacgt gtttcgccac cgccgcagcc ataataccta
    19501 tcggacctgc accagaaacc agcacatctt cgcccaccag atcgaacgac agcgcggtat
    19561 gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc gtcggagata ttgtctggga
    19621 ttttgaaggc gttgaacgcc gggatcacca gatattcggc gaagcagccc ggacggttaa
    19681 cgccgacgcc gatcgtgttg cggcacagat gggtacgacc gccacgacag ttgcgacagt
    19741 gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc gattttgaag cctttcactt
    19801 cctgaccaat acctaccact tcaccgacat attcatggcc tacgaccatc ggcaccggga
    19861 tggttttttg cgaccactca tcccagttat agatgtgaac gtcagtcccg cagatggctg
    19921 ttttacggat tttaatcagc agatcgttat gcccgagttc cggtacagga acgtcggtca
    19981 tccagatgcc ctcttccgct ttcagtttgg ataacgcttt catctcacat cctcaggcga
    20041 taacgcccag ttgtttacca atacgcgtga atgcttctac tgcacgcgta atttgctcag
    20101 gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg acctttcgga acgaccggat
    20161 agaagaaacc ggtgacgtaa atgccctctt tttgcagctc acgggcaaat ttctgcgcca
    20221 ctactgcatc gccaagcatg accggaataa tggcatgatc ggctcccgcc agggtaaagc
    20281 ccgccgccga catttgctca cggaactggc gcgcgttcgc ccacagacgg tcacgcagtt
    20341 cgctgcccgc ttcgaccatc tccagtactt tgatggacgc ggcaacaatg gccggtgcca
    20401 gcgagttgga gaacaggtac ggacgagaac gctggcgcag ccactcaacc acctctttgc
    20461 gcgccgcggt ataaccacca gaagccccgc ccagcgcttt accaagcgta ccggtgataa
    20521 tatcgacccg gcccatcaca tcgcagtatt catgggaacc acgaccattt tcaccgacaa
    20581 aaccgaccgc gtgggagtcg tctaccatca ccagggcatc gtatttatct gccagatcgc
    20641 aaacgccctt cagattggca atcacgccgt ccatcgagaa tacaccatcg gtggcaatca
    20701 gcacatgacg cgcaccggct tcacgcgctt ctttcagacg agcttcaagc tcctgcatat
    20761 cgttgttggc atagcgatag cgtttagctt tacacagacg cacaccatca ataatagacg
    20821 cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc cagcagcgtt tcaaacaggc
    20881 caccgttagc atcaaagcag gaagagtaga gaatcgcatc ttccatcccc aggaaggccg
    20941 ccagtttttg ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa cgcaccgaag
    21001 ccatgccgaa accgtgagaa tccattcccg cctttgccgc cgcaatcaga tcaggatgat
    21061 tcgccagccc gagatagttg ttggcacaaa agttaatgac gtggcttcca tcagccacag
    21121 tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac ccttccgccc
    21181 gtgcggtttc cagatcgttg gttaactgct gataaaattc tccacgcatt gcgattctcc
    21241 agacagggca aattccagca catattaccc aaacttatag gtcgcgacga gatgacgcgt
    21301 taacacttct gcaaaattca ggataaataa cgtgtaactt gatgtctctc aacacctaca
    21361 gatagtagaa aaactgtaca gttttggctg ggcataatga tgattgattt tactgcattg
    21421 atataaaaag aaaaaatgat gccaaagcat catttttcct atctcagttg gtatcaaaac
    21481 gctacccact aatggaggga aacctatttt cttgaaagga aaattctcag cgcctgcatt
    21541 tttgccccta aagtgttact cctgaagcag cgaaacaaat cgaaccattt tcctctcccc
    21601 agatagttta ggttgaagag atcggggcgt ttgtaatagc acatgacaaa aatgccctgg
    21661 tcatcatcca caatgttatt atttagtgtt attttttgac tttccagaac taatttatag
    21721 aattctttcc atttatgctg tgaaccaaca attgcgcccc caataatata tacatgattg
    21781 ccaatcatga aatcaaaaac ctgttgttga gatgtgacag taagcccttt ttttatagtg
    21841 aaaagatgca tcttactttc atcaaatggg aaatcccaaa ttttcaatcc tcgagttaca
    21901 tttggcttac ggcaatagcc aaaatctatc cacgcaacaa gaggcgtttt cactaagccc
    21961 atgttgatag ctttatttac aaaataggcc ttaaggttac ataccaaaac atactctggt
    22021 gaccagtact ctgggttttt taattgtcga ggctcaagtc tatttgtaaa cgactcatct
    22081 ttttgaattt tttcgatacg acttctgata tatctaaatt tcttttttat atcgataacg
    22141 ataaccgttg ttggttttcc gttgcgaatc gcttcaaccc ttggtttcaa atcgggagaa
    22201 gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct caaagtaact aaaataaaca
    22261 tcaactgaac gagctagttt ttcacggaac cctttattag cagtccaatc ccctctacca
    22321 atgtcaaaat atgcagtgat aattgtggtg gatgatttca ttactaataa gaccattaat
    22381 ataaaaaaag aatt
//
LOCUS       DAZGCS010000078        22065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGCS010000078 DAZGCS010000000
VERSION     DAZGCS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.078"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..107
                     /locus_tag="ACHK01_003256"
     CDS             3..107
                     /locus_tag="ACHK01_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO8098167.1"
                     /translation="MLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            86..190
                     /gene="speFL"
                     /locus_tag="ACHK01_003257"
     CDS             86..190
                     /gene="speFL"
                     /locus_tag="ACHK01_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO8098168.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            501..2699
                     /gene="speF"
                     /locus_tag="ACHK01_003258"
     CDS             501..2699
                     /gene="speF"
                     /locus_tag="ACHK01_003258"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO8098169.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2696..4015
                     /gene="potE"
                     /locus_tag="ACHK01_003259"
     CDS             2696..4015
                     /gene="potE"
                     /locus_tag="ACHK01_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO8098170.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4071..5711)
                     /gene="pgm"
                     /locus_tag="ACHK01_003260"
     CDS             complement(4071..5711)
                     /gene="pgm"
                     /locus_tag="ACHK01_003260"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO8098171.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5737..6282)
                     /gene="seqA"
                     /locus_tag="ACHK01_003261"
     CDS             complement(5737..6282)
                     /gene="seqA"
                     /locus_tag="ACHK01_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO8098172.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6467..7231
                     /gene="ybfF"
                     /locus_tag="ACHK01_003262"
     CDS             6467..7231
                     /gene="ybfF"
                     /locus_tag="ACHK01_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8098173.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7371..7664
                     /gene="ybfE"
                     /locus_tag="ACHK01_003263"
     CDS             7371..7664
                     /gene="ybfE"
                     /locus_tag="ACHK01_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO8098174.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7804..8334
                     /gene="fldA"
                     /locus_tag="ACHK01_003264"
     CDS             7804..8334
                     /gene="fldA"
                     /locus_tag="ACHK01_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO8098175.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8544..8630
                     /gene="uof"
                     /locus_tag="ACHK01_003265"
     CDS             8544..8630
                     /gene="uof"
                     /locus_tag="ACHK01_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO8098176.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8623..9069
                     /gene="fur"
                     /locus_tag="ACHK01_003266"
     CDS             8623..9069
                     /gene="fur"
                     /locus_tag="ACHK01_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO8098177.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9153..9479)
                     /gene="chiQ"
                     /locus_tag="ACHK01_003267"
     CDS             complement(9153..9479)
                     /gene="chiQ"
                     /locus_tag="ACHK01_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO8098178.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9529..10935)
                     /gene="chiP"
                     /locus_tag="ACHK01_003268"
     CDS             complement(9529..10935)
                     /gene="chiP"
                     /locus_tag="ACHK01_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO8098179.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11381..13045)
                     /gene="glnS"
                     /locus_tag="ACHK01_003269"
     CDS             complement(11381..13045)
                     /gene="glnS"
                     /locus_tag="ACHK01_003269"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO8098180.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13249..15195)
                     /gene="nagE"
                     /locus_tag="ACHK01_003270"
     CDS             complement(13249..15195)
                     /gene="nagE"
                     /locus_tag="ACHK01_003270"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO8098181.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDY
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15528..16328
                     /gene="nagB"
                     /locus_tag="ACHK01_003271"
     CDS             15528..16328
                     /gene="nagB"
                     /locus_tag="ACHK01_003271"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8098182.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKICSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16388..17536
                     /gene="nagA"
                     /locus_tag="ACHK01_003272"
     CDS             16388..17536
                     /gene="nagA"
                     /locus_tag="ACHK01_003272"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8098183.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17545..18765
                     /gene="nagC"
                     /locus_tag="ACHK01_003273"
                     /gene_synonym="nagR"
     CDS             17545..18765
                     /gene="nagC"
                     /locus_tag="ACHK01_003273"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO8098184.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18813..19565
                     /gene="nagD"
                     /locus_tag="ACHK01_003274"
     CDS             18813..19565
                     /gene="nagD"
                     /locus_tag="ACHK01_003274"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO8098185.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19822..21486
                     /gene="asnB"
                     /locus_tag="ACHK01_003275"
     CDS             19822..21486
                     /gene="asnB"
                     /locus_tag="ACHK01_003275"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO8098186.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21867..21943
                     /locus_tag="ACHK01_003276"
     tRNA            21867..21943
                     /locus_tag="ACHK01_003276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21901..21903,aa:Met,seq:cat)
     gene            21952..22036
                     /locus_tag="ACHK01_003277"
     tRNA            21952..22036
                     /locus_tag="ACHK01_003277"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21986..21988,aa:Leu,seq:tag)
BASE COUNT         5413 a         5559 c         5567 g         5526 t
ORIGIN      
        1 ttttgttgat cttcttcaca gattatagcc atttcatgga tagaataact ctaccttcaa
       61 ctgacacagc aagaggtaaa ggtaaatgga aaataataac cgcttaatgc ctcatataag
      121 gcggacaacc catatcatga agtttgccca tcgtaatagt tttgactttc atttctttaa
      181 tgcccgctag tcttctgact aaagggcacc ccaacgtaca ggtctccctg actttaagca
      241 ttacaggtta atacctgtat tcctcggtgc tcatatactg ctaacccttt taaactctaa
      301 ataattcgag tcgcagcact tgcaacttga ggtatgacga gtatagccag ttaccgggct
      361 ggtctgggtt attgcatctg caaaaagcaa actactgatt tatttatcag cggtggagct
      421 ttgctttttt tccggcgtga tcgatttctc ctttgagaaa ttgaggacct gctattacct
      481 gaaataaaga gatgaacaaa atgtcagaat taaaaattgc cgttagtcgt tcttgcccgg
      541 attgtttttc cactcatcgt gcatgcgtga atatagacga aagtaattat attgacgttg
      601 ccgccattat tttatcagtc agtgatgttg aacgtggaaa actcgatgaa atagacgcta
      661 ctggctatga cattcctgtt tttattgcaa cggaaaatga agaacgtgta cccgcagaat
      721 atctgccacg tatttctggt gtcttcgaac attgcgaatc acgtaaagag ttttatggac
      781 gtcagttaga aaccgctgcc agccattatg aaacgcaact gcgcccgccc ttcttccgcg
      841 cactggtcga ttatgttaat cagggcaata gtgcttttga ctgccctggt catcaggggg
      901 gtgagttttt ccgtcgccat ccggcaggca atcagttcgt ggaatacttt ggcgaaatgc
      961 tcttccgctc tgacttgtgt aatgctgacg tagcaatggg cgatctgctg attcatgaag
     1021 gtgcgccatg tattgcacaa cagcatgcgg ctaaagtctt taatgccgat aaaacctatt
     1081 tcgttttaaa cggtacttca tcttctaata aagtggtctt aaacgcattg ctgacgccgg
     1141 gcgatctggt gttgtttgac cgcaataacc ataaatctaa ccaccacggt gcgttgctac
     1201 aggctggcgc gacaccggtt tatctggaaa ccgcacgtaa cccgtatggc tttatcggtg
     1261 gcattgatgc gcactgtttt gaagaaagtt acctgcgtga gctgatcgcg gaagtcgcac
     1321 cgcagcgggc aaaagaggct cgtcctttcc gcctcgctgt gattcagtta ggcacctacg
     1381 acggtacgat ttataacgcc cgccaggtgg tggataaaat tggtcatctg tgtgactaca
     1441 ttctgtttga ctccgcatgg gttggctatg agcaatttat cccgatgatg gctgactgtt
     1501 cgccgctgtt gctggatctt aatgagaacg atccgggtat tctggttacg caatccgtac
     1561 ataaacagca ggctggtttt tctcaaactt cacaaatcca taaaaaagac agccacatca
     1621 aagggcaaca gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg atgcatgcct
     1681 ccaccagccc gttctatccg ctgtttgccg cactggatat caacgccaaa atgcatgaag
     1741 gtgtcagcgg tcgtaatatg tggatggatt gtgtggtaaa tggcattaat gcccgcaagc
     1801 tgatcctcga taactgtcag catattcgtc cgttcgtacc tgaactggtg gatggtaaac
     1861 cctggcagtc gtatgaaaca gcgcaaattg cggttgatct gcgcttcttc cagtttgtac
     1921 caggtgagca ctggcactct tttgaaggct atgcagagaa tcaatacttt gtcgatccat
     1981 gcaaactgtt gctgacaacc ccaggtattg atgcacgtaa cggcgaatat gaagcgttcg
     2041 gtgtacccgc gacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat
     2101 gcgatcttaa ctccatcctc ttcctgctga ctccggcaga agatatggcc aagcttcagc
     2161 aacttgttgc cctgctggta cgcttcgaaa aactgcttga gtccgacgcg ccattagcag
     2221 aagtgctgcc ttccatctac aaacagcatg aagagcgcta cgccggttat accctgcgtc
     2281 agttgtgtca agaaatgcat gatttgtatg cccgtcacaa cgtgaaacaa ctgcaaaaag
     2341 agatgttccg taaggaacac ttcccacgcg taagcatgaa tccgcaagaa gccaactacg
     2401 cctatttacg cggtgaagtg gagctggttc gtctgccgga tgcagaaggc cgtatcgctg
     2461 ccgaaggtgc gcttccttat cctccgggtg tgctgtgtgt tgttccgggt gaaatctggg
     2521 gtggtgctgt tttgcgttac ttcagcgctc tggaagaagg gatcaacctg ctgccaggtt
     2581 ttgcaccgga gctgcagggt gtctatatcg aagaacatga tggtcgtaag caagtttggt
     2641 gctatgtcat caagcctcgt gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag
     2701 tcaggctaaa tcgaacaaaa tgggcgtcgt tcagttaacc atactgacga tggtcaacat
     2761 gatgggctcc ggtatcatca tgctgccgac aaagcttgcc gaagttggga caatttcaat
     2821 tatctcctgg ctggtgacag ccgtaggctc aatggcactg gcatgggcat tcgcaaaatg
     2881 cggtatgttc agccgtaaat caggcggtat gggcggttat gcggagtatg ctttcggtaa
     2941 atccggtaac tttatggcga actataccta cggcgtctcg ctgctgattg ctaacgtcgc
     3001 gattgctatt tcggcggttg gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt
     3061 gcagattggt cttgcgacca tcggggtgct gtggatttgt accgtggcta actttggtgg
     3121 tgcgcgcatt accgggcaaa tcagtagcat taccgtgtgg ggggtcatta ttccggtggt
     3181 aggcctgtgc attatcggct ggttctggtt tagcccgacg ctttacgttg attcctggaa
     3241 tccgcatcat gcaccgttct tcagtgcagt aggttcttcc atcgctatga cgctgtgggc
     3301 ttttctcggt ctggagtctg cgtgtgcgaa tactgatgta gtggaaaacc cggaacgtaa
     3361 tgtgccaatc gcggtactcg gcggtacgtt aggtgcggcg gtgatttata tcgtctccac
     3421 caacgtgatt gcagggattg tgcctaatat ggagctggca aattcaacgg caccatttgg
     3481 cctggccttc gcacagatgt tcacaccaga agtgggtaaa gtcattatgg cgctgatggt
     3541 gatgtcctgc tgcggttcgc tgcttggctg gcagttcacc attgcccagg tgtttaaatc
     3601 ttcatctgat gaaggctact tccctaaaat tttctcccgt gtaaccaaag tggatgcacc
     3661 ggtgcaggga atgttgacca ttgtgattat ccagagcggg ttggcgctga tgacgattag
     3721 cccgtcgcta aacagtcagt tcaacgtgct ggttaacctg gccgtggtga ccaatatcat
     3781 tccgtatatt ctgtcaatgg cggcattagt cattattcag aaggtcgcta atgtgccccc
     3841 ctcaaaagcg aaagttgcaa actttgttgc ttttgttggc gcgatgtata gcttttatgc
     3901 gctgtactca tccggggaag aagccatgct gtacggttcc atcgtgacct tccttggttg
     3961 gacactgtat ggtctggtat caccacgctt tgaactgaaa aataaacacg gttaaaaaag
     4021 caaagtaaaa aagggcgatc ttgcgaccgc ccttttttta ttaaatgtgt ttacgcgttt
     4081 ttcagaactt cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg atgttcttca
     4141 ccgaggaagc tttcgcagta gatcttatat gcgtcttccg tgcctgacgg acgcgcggcg
     4201 aaccagccgt tgtcagtcat cactttcaga ccgccgatag aagcaccatt acccggtgcc
     4261 gccgtcagac gtgcggtgat cgggtcacct gccagggtgc tggcgctcac catttccgga
     4321 gacagcttag acaacgccgc tttttgtgcg gaagtcgcag cggcctgcaa acggttgtag
     4381 ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt cttaccggtg
     4441 acagcggtga tttccgccgc cagcagacac ataatgatgc cgtctttatc ggtggaccac
     4501 ggcgtgccgt cgaaacgcag gaaggaagcc ccggcgctct cttcgccgcc aaagccgaaa
     4561 ctgccgtcga acagaccatc aacaaaccat ttgaatccta ccgggacttc taccagttta
     4621 cggcccaagg cgttgaccac acggtcgatc atcgcagaag aaaccagcgt tttaccgaca
     4681 gcaacatctt tgccccactg cggacgatgc tggaacaggt agttgatcgc caccgccagg
     4741 tagtggttcg gattcatcaa acctgccgga gtgacgatac cgtgacggtc ataatccggg
     4801 tcgttagcaa acgccagatc gaacttatca cgcagtgcca gcaggcccgc catcgcacac
     4861 tcggaggagc agtccatacg gatcgcgccg tctttatcaa ggtgcataaa gcggaaggtt
     4921 tgatcgacct gatcgttaac gatggtcagg ttgaggttgt aatactcgcc aatacgcttc
     4981 cagtattcga taccggaacc gcccagcgga tcaacgccaa gcgtcaggcc cgctttctga
     5041 atcgccgcca tatcaacgat atcggccaga ccttccacaa acggctgcac cagatcctgc
     5101 tctttcacat gaccggatgc catcgcttcg tcgagagaga tacgcttcac gcctttcagg
     5161 ccatcggcca tcagtgcgtt ggccctgtct tccaccactt tggtgacgtt ggtatcagcc
     5221 gggccaccat ttggcgggtt gtacttgata ccaccatctt ccggcgggtt atgggacggt
     5281 gtaatcacga taccgtctgc cagcgggcca ccttttttat tgtgaaccag gatggcattg
     5341 gaaacggcag gcgtcggagt gaatccattg ttttcctgca caatgacatc aacgccgttc
     5401 gctgccagca cttccagtac ggaaatgaac gcaggctcgg acagggcgtg agtatcttta
     5461 cccacatagc aagggccagt gatgccattt ttcgcacgtt cttcagcaat tgcctgagcg
     5521 atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg cactgccacg gtgaccggaa
     5581 gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac ataatattgc
     5641 gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg caggttggcc tgcacgattg
     5701 tggattgcca ttgctttgtc ctttgtctgc aacgttttag atagttccgc gaaccttctc
     5761 aatcaattcc gccgggaatt gcatcgactg catgatgtgt tcgatcatgc tgcatttacg
     5821 gccggtgttg gtgttggtga tcacccaata cggcgtgcct ggcacatgtt tcggcttggt
     5881 ctgattacca tttttcagca gcgtttgttc atctgccgca aagtaaacgc gtgtacgacc
     5941 gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca agagaatata gtgtagacaa
     6001 cagcagcata aagcgattga ccgctcgctt ttgctctgcg tattcgtccg aaagcagaag
     6061 ttcacgcatt gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt cgacgatagc
     6121 aggtgacgca acgcgaacct ctttcgtcac cggagcagca ggctgtgatg aggcggaaaa
     6181 tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag tgtggctggc
     6241 aatatagctg tagagttcat catcaacttc aatcgttttc atcttaatcc agtgcagtgt
     6301 cttagctgaa tacaagtcgc caggagtata tgaataaatc ccggcagcgg atagcgtcaa
     6361 gcctggcttg cagaaaaact cggaataaac cttatttgtt gcagccctgt ggcacaatgg
     6421 tcaacataat accctaaccc gacaacgcga aaaaaagaac tttgccatga aattgaatat
     6481 ccgcgcgcaa actgcacaaa accagcacaa taattctccc atcgttcttg tccatggtct
     6541 gtttggtagc ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg atcacaatat
     6601 catccaggtt gatatgcgta accacggtct ttcaccgaga gatccggtaa tgaattaccc
     6661 ggcgatggcg caggatcttg ttgatactct ggatgcacag cagatcgaca aagcaacatt
     6721 tatcggtcac tccatgggcg gtaaagcggt aatggcactt actgcactgg cccccgatcg
     6781 catcgataaa ctggtggcga tcgatatcgc accggtcgac tatcacgtac gccgtcatga
     6841 tgagattttc gcggctatca acgcggtcag tgaatcggac gcacaaactc gccagcaagc
     6901 agcagcaata atgcgccagc atcttaatga agaaggggtg attcagtttc tgttgaaatc
     6961 ttttgttgac ggggagtggc gctttaacgt gccggtattg tgggatcagt atccgcatat
     7021 tgtgggttgg gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc ctggcggcaa
     7081 ttctccgtat gttagcgagc agtaccgtga tgatttactg gcacaatttc cacaggcacg
     7141 ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg atgcggtatt
     7201 acgcgctatc cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc agcgtccgcg
     7261 tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat ggcgcgctat
     7321 ccatccgggc agaggcctgg ctgattgttt cccccgaagt caccaagatc atggccaaag
     7381 aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg ggacgaccga
     7441 aaactaatcc gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac cagctaaaac
     7501 gcgacaaagt acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa gctgtcgagg
     7561 cgctgaacga gctggcggag tcgcgcaata tgagccgtag cgaactgatc gaagagatgt
     7621 taatgcagca actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc actttcatgt
     7681 agcacagtgt gcagtcctgc tcgtttgctg attccgcaag actgcctgtt ctgctatgat
     7741 tgcctttatc cgtgggcaat tttccacccc catttcaata agtttcaaga ggttatttca
     7801 ctcatggcta tcactggcat ctttttcggc agcgacaccg gtaataccga aaatatcgca
     7861 aaaatgattc aaaaacagct tggtaaagac gttgccgatg tccatgacat tgcaaaaagc
     7921 agcaaagaag atctggaagc ttatgacatt ctgctgctgg gcatcccaac ctggtattac
     7981 ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc tcgaagagat tgatttcaac
     8041 ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag attacgccga atatttctgc
     8101 gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg gtgcaaccat cgttggtcac
     8161 tggccaactg cgggctatca tttcgaagca tcaaaaggtc tggcagatga cgaccacttt
     8221 gtcggtctgg ctatcgacga agaccgtcag ccggaactga ccgctgaacg tgtagaaaaa
     8281 tgggttaaac agatttctga agagttgcat ctcgacgaaa ttcttaatgc ctgatgtgat
     8341 gcggcgtaga ctcatgtcta cgccgtatta atagataatg ccaatcaaaa taattgctac
     8401 aaatttgtaa cttttgctgt tgtacctgta caatgtcccg gtgttcaagt ggccttgccg
     8461 ttgtaaatgt aagctgtgcc acgtttttat taacaatatt tgccagggac ttgtggtttt
     8521 catttaggcg tggcaattct ataatgatac gcattatctc aagagcaaat tctgtcactt
     8581 cttctaatga agtgaaccgc ttagtaacag gacagattcc gcatgactga taacaatacc
     8641 gccctaaaga aagctggcct gaaagtaacg cttccacgtt taaaaatcct ggaagttctt
     8701 caggagccgg acaaccatca cgtcagtgcg gaagatttat acaaacgtct gatcgatatg
     8761 ggtgaagaaa ttggtctggc tacggtatat cgcgtactga accagtttga cgacgctggt
     8821 atcgtcaccc gccacaattt tgaaggcggt aaatccgtat ttgaactgac acagcaacat
     8881 caccacgatc acctgatttg cctcgactgc ggcaaggtta tcgaatttag tgatgattcc
     8941 atcgaagcgc gtcagcgtga aattgccgca aaacatggca ttcgcctgac taaccacagt
     9001 ctctatcttt acggtcactg tgccgaaggc gattgccgcg aagatgagca cgcgcacgaa
     9061 ggcaaataag ccagcctgaa cgagaaaagc caacctgcgg gttggctttt ttatacaagg
     9121 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg tatttcctgc
     9181 cagaccttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
     9241 cactgctgat aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc
     9301 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
     9361 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
     9421 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
     9481 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     9541 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc
     9601 gccgccccag cttgggatgt cagagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     9661 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc
     9721 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
     9781 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     9841 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttggcgttga agtcggaacg
     9901 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
     9961 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
    10021 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    10081 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
    10141 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    10201 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    10261 accgatggag tgcaggtaat caactttggt ggttttatcg ttctgataaa actcatccat
    10321 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc
    10381 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
    10441 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    10501 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    10561 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    10621 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    10681 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    10741 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    10801 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    10861 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    10921 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    10981 agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    11041 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    11101 gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt
    11161 gcagaagaat aaaagcgtga atttccagca agatgaaagc ggttacattt tatttaatta
    11221 tggtttgtga ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    11281 aaaagcagaa aaaacgccgc agagtgcggc gtttgggatt gtttgctatt tcttgccgga
    11341 tgcgacgtaa atgcctcacc cggcctggta gaacttaaaa ttactcgcct actttcgccc
    11401 aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttccgctgtg gaatggcggc
    11461 tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca accgcatctt
    11521 tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta atcaccgaca
    11581 ggaaatcatc cgcagcacca gggttcggca cgctgaacag acggtcgtac agacggattt
    11641 caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact ttacgaccat
    11701 ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattgc
    11761 cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc agacgcactt
    11821 ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat
    11881 cgatccaaat ctcaccgcta aacggcacct gacgactccc catttccggt ttgttcggat
    11941 ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg
    12001 gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg atgcaggatt
    12061 ccagcgatgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact
    12121 cacgaataga agcagcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg
    12181 ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca
    12241 tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcgggtga acaggaatgg
    12301 tgatgttgtc cagcacccag tcgtacaggc gacggttgtc ctgaaactca agcgtacaca
    12361 gagagtgcgt aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg tacatcgggt
    12421 agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatacgg tacagcaccg
    12481 gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg caggctttac
    12541 cttcttcaaa accaccggca cgcatttttt cgaacagcgc caggttctct tcaacgctgc
    12601 ggtcgcggta cgggctgttt ttacccggtt gcgtcagggt gccgcggtat tcgcggatct
    12661 gttccggcgt cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg
    12721 cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta
    12781 accactctac gtcgtttttg atcgactcaa catactcgat atcttctttt accgggttag
    12841 tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc
    12901 agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt
    12961 gtactgtggt gtgcttacca ctggccagat cttcatcgat gatctgacgg ataaagttag
    13021 tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg
    13081 cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat tgataatcta
    13141 acaaggatta tggggatgat tgatgaaaaa aaaaagcggc agagaagatc tccaccgctt
    13201 gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat
    13261 ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact
    13321 gaaatagtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt tagcgttcag
    13381 gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcac cctcttccac
    13441 cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat
    13501 ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat
    13561 tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc
    13621 caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt
    13681 aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac
    13741 aggcgcggca gttgccggag ttgcttcagc tgacgcagcg gctaccggac cacgggcaac
    13801 gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt
    13861 ctgtttgttc agtttcacta caccagaagc acccagacgt ttacacatcg tatcgttgac
    13921 gcgggcagag tcagccacgg tcaggcgcag acgggtgata caggcgtcaa ttgctttcag
    13981 gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc
    14041 agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt
    14101 tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac
    14161 aacacccata accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa
    14221 cgcgtagtcg atagcccccg cagagaaaga gaagccagca tggataccca gcagtgttgc
    14281 cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa
    14341 catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga
    14401 aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag
    14461 cgccgcaccc ggcagaccga acatcataat cgggaagaag ccggacatga acatccccgc
    14521 ggtgccgtca ccggcataga agcggttgat atcaccgtgg aaaaccgtac ccgccgcgtt
    14581 ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg
    14641 gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat
    14701 ccactcgcct cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc
    14761 cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc cgccgaagaa
    14821 actcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag
    14881 accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc
    14941 tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt
    15001 cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc
    15061 gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag
    15121 tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa
    15181 acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta
    15241 tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg
    15301 ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt
    15361 ttgtcaccaa atatcgttat catcactccc ttttactggc taaagcagaa aacttatttt
    15421 atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa
    15481 gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc
    15541 ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca
    15601 atgcgttcaa accgactgcc gatcgtccgt ttgttctggg cctgccgact ggcggcacgc
    15661 cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc
    15721 acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct
    15781 actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca
    15841 accttctcaa cggcaacgcc ccggatatcg acgccgaatg ccgccagtat gaagaaaaaa
    15901 tctgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg
    15961 catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc ttgactcatg
    16021 acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg
    16081 ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg
    16141 gtagccagaa agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga
    16201 ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca
    16261 tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag
    16321 gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg
    16381 ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga
    16441 ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc
    16501 gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt
    16561 gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga
    16621 aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac
    16681 gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct
    16741 ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt
    16801 aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt
    16861 cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc
    16921 ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc
    16981 gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt acctttgcca cccatctgta
    17041 caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga
    17101 agctgacatt tattgcggta ttattgctga tggcctgcat gttgattacg ccaacattcg
    17161 taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc
    17221 aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact
    17281 ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg
    17341 taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta
    17401 tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc
    17461 caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga
    17521 ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt
    17581 gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca
    17641 atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt
    17701 acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg
    17761 ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg
    17821 cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa
    17881 gaacattacc cgctgccgga acgtacccag caaacgctgg aacatgccct gttgaatgcc
    17941 attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc gatttcggtg
    18001 atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag
    18061 gtagaaaact gggggctggt agaagcactg gaagaacgtt ttaaagtgac ctgtttcgtt
    18121 ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc
    18181 gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg
    18241 cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg
    18301 ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc
    18361 gctattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg
    18421 gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg
    18481 gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta
    18541 tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc
    18601 cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg
    18661 gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc
    18721 gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg
    18781 gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata
    18841 tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg
    18901 ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc
    18961 aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc gtgttttata
    19021 cctctgcgat ggcgactgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg
    19081 tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga
    19141 accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag
    19201 cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc
    19261 gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca
    19321 aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcggcatta aacaaaatgc
    19381 aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgcaccgac attctggcgg
    19441 gcttccaggc gggtctggag acgattctgg tgctttctgg tgtttcatcg ctcgacgata
    19501 tcgacagtat gccgttccgc ccaagctgga tttacccgtc ggtcgctgaa atcgacgtta
    19561 tctgaatagc aaccacgtct cagggcgtgg tttcttattt ttacccatca ttaaaatcat
    19621 cccattcaat aaaactcgcc aaaaattatc gatccatcaa taaatacatc accacaccct
    19681 cccctttttc acaaatcagc aatcaccatt acgtttttta tttttgcact gccaaatcgc
    19741 ttgcgcacgg tgctgcttta cggcattttt ttaaacaagc aaacacaaca agcaacaaat
    19801 accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
    19861 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
    19921 gactggtccg gtatttatgc cagcgataac gccattctcg cccacgaacg tctgtcaatt
    19981 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggcg
    20041 gtaaacggtg aaatctacaa ccaccaggca ctgcgcgccg aatatggcga tcgttatcag
    20101 ttccagaccg gatctgactg cgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa
    20161 tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgca
    20221 tatctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgagcac
    20281 ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc cagtttgccg cacgattaaa
    20341 gagttcccgg cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat
    20401 cgcgactggt tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt
    20461 caggcactgg aagattccgt taaaagccat ctgatgtctg acgttcctta cggtgtgctg
    20521 ctctcgggtg gtctggattc ctcaattatt tccgcgatca ccaagaaata cgccgcccgt
    20581 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc aattacactc ctttgctgta
    20641 ggtctgccgg gttcaccgga tctgaaagca gcccaggaag tggcaaacca tctgggcacg
    20701 gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt
    20761 taccacatcg aaacctatga tgtgacaact attcgcgctt caacaccgat gtatttaatg
    20821 tcgcgtaaga tcaaggcaat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa
    20881 gtgtttggcg gttatcttta cttccacaaa gcaccgaatg ccaaagaact gcatgaagag
    20941 acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgcgccaa caaagcgatg
    21001 tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg
    21061 atgcgcatta acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
    21121 cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc
    21181 tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt
    21241 tctgatcagc aactggaaac tgcccgcttc cgcttcccgt acaacacgcc aacctcaaaa
    21301 gaagcgtatc tgtaccggga gatctttgaa gaactgttcc cgcttccgag cgccgctgag
    21361 tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag
    21421 gcgttcaaga aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat
    21481 aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct
    21541 tgttaattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac
    21601 tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa
    21661 tttcatcctg tacgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat
    21721 aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat
    21781 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    21841 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    21901 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg
    21961 gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt
    22021 ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggt
//
LOCUS       DAZGCS010000079        21866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGCS010000079 DAZGCS010000000
VERSION     DAZGCS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.079"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..287)
                     /locus_tag="ACHK01_003278"
     CDS             complement(<1..287)
                     /locus_tag="ACHK01_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098187.1"
                     /translation="MDITCLECGNVLDDPTVACDKCGATPHVVVLGKQSYFPIGAVTA
                     NLEKNDSRAFDYRLGEVWDLKNEVTSEFITRIEKKFSRKNKFHNFLDSDHNP"
     gene            579..905
                     /locus_tag="ACHK01_003279"
     CDS             579..905
                     /locus_tag="ACHK01_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO8098188.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            909..1385
                     /locus_tag="ACHK01_003280"
     CDS             909..1385
                     /locus_tag="ACHK01_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO8098189.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPRSQDSVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            1382..1819
                     /locus_tag="ACHK01_003281"
     CDS             1382..1819
                     /locus_tag="ACHK01_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8098190.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKAASII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGINRITAQVNYLQEYIRTQCLK"
     gene            2249..2500
                     /locus_tag="ACHK01_003282"
     CDS             2249..2500
                     /locus_tag="ACHK01_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098191.1"
                     /translation="MSSTKLVKTILFHGDNKYEVYATSEYADVDRIIHYKLRSCKNTD
                     GSDGKSESIWVVYNPAVQLPDDLNYKKVSNIAPYLEKLQ"
     gene            2713..3063
                     /locus_tag="ACHK01_003283"
     CDS             2713..3063
                     /locus_tag="ACHK01_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8098192.1"
                     /translation="MPPRIPKACRARGCRNTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRSGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            3189..3683
                     /locus_tag="ACHK01_003284"
     CDS             3189..3683
                     /locus_tag="ACHK01_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO8098193.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEENLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            3680..5413
                     /locus_tag="ACHK01_003285"
     CDS             3680..5413
                     /locus_tag="ACHK01_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8098194.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVVCCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNINRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTRCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            5425..5607
                     /gene="ymfR"
                     /locus_tag="ACHK01_003286"
     CDS             5425..5607
                     /gene="ymfR"
                     /locus_tag="ACHK01_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFO8098195.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQLSVGGGK"
     gene            5607..6848
                     /locus_tag="ACHK01_003287"
     CDS             5607..6848
                     /locus_tag="ACHK01_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8098196.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVEPKLNSSWEPIYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            6826..7476
                     /locus_tag="ACHK01_003288"
     CDS             6826..7476
                     /locus_tag="ACHK01_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO8098197.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            7491..8696
                     /locus_tag="ACHK01_003289"
     CDS             7491..8696
                     /locus_tag="ACHK01_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8098198.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            8746..8946
                     /locus_tag="ACHK01_003290"
     CDS             8746..8946
                     /locus_tag="ACHK01_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098199.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            8949..9272
                     /locus_tag="ACHK01_003291"
     CDS             8949..9272
                     /locus_tag="ACHK01_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8098200.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTDVEKMELPMSFNWLVGPY
                     RYFPQ"
     gene            9269..9679
                     /locus_tag="ACHK01_003292"
     CDS             9269..9679
                     /locus_tag="ACHK01_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8098201.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            9654..10160
                     /locus_tag="ACHK01_003293"
     CDS             9654..10160
                     /locus_tag="ACHK01_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098202.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRGHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            10157..10717
                     /locus_tag="ACHK01_003294"
     CDS             10157..10717
                     /locus_tag="ACHK01_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098203.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            10726..10896
                     /locus_tag="ACHK01_003295"
     CDS             10726..10896
                     /locus_tag="ACHK01_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO8098204.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDDKL"
     gene            10880..12376
                     /locus_tag="ACHK01_003296"
     CDS             10880..12376
                     /locus_tag="ACHK01_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO8098205.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLSAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            12376..12732
                     /locus_tag="ACHK01_003297"
     CDS             12376..12732
                     /locus_tag="ACHK01_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO8098206.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRMVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVGKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            12732..13001
                     /locus_tag="ACHK01_003298"
     CDS             12732..13001
                     /locus_tag="ACHK01_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO8098207.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            13143..14978
                     /locus_tag="ACHK01_003299"
     CDS             13143..14978
                     /locus_tag="ACHK01_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8098208.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRLMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFETGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            15039..16367
                     /locus_tag="ACHK01_003300"
     CDS             15039..16367
                     /locus_tag="ACHK01_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO8098209.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNAGTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVASI
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            16364..17443
                     /locus_tag="ACHK01_003301"
     CDS             16364..17443
                     /locus_tag="ACHK01_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO8098210.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTQTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            17443..17991
                     /locus_tag="ACHK01_003302"
     CDS             17443..17991
                     /locus_tag="ACHK01_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8098211.1"
                     /translation="MRTIETMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPRARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            17991..18416
                     /locus_tag="ACHK01_003303"
     CDS             17991..18416
                     /locus_tag="ACHK01_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO8098212.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            18403..19461
                     /locus_tag="ACHK01_003304"
     CDS             18403..19461
                     /locus_tag="ACHK01_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8098213.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            19452..20036
                     /gene="ymfQ"
                     /locus_tag="ACHK01_003305"
     CDS             19452..20036
                     /gene="ymfQ"
                     /locus_tag="ACHK01_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFO8098214.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLSGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            20040..20786
                     /locus_tag="ACHK01_003306"
     CDS             20040..20786
                     /locus_tag="ACHK01_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO8098215.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SPAIGVPFFWPSAAMPNTVIDSWSSMVFLKFNGAKFSATDYPVLAKVFPSLVLPEARG
                     DFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAINFHDGIAGNQPGFSRF
                     NFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
     gene            20786..21379
                     /locus_tag="ACHK01_003307"
     CDS             20786..21379
                     /locus_tag="ACHK01_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8098216.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGKVAVFTGKGWSHEEDHRNETVYSTENGAAVTVDYIGAIKDGYVT
                     LSPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTVMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTVTDTSTAPDIIWPELPEA"
     gene            complement(21351..21770)
                     /locus_tag="ACHK01_003308"
     CDS             complement(21351..21770)
                     /locus_tag="ACHK01_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFO8098217.1"
                     /translation="MDRYFYSQKENGFFTDLNKAPSDAVEITTDEWLSLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALAELKKGKLINEANEHMNSRQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
BASE COUNT         5194 a         5387 c         6354 g         4931 t
ORIGIN      
        1 gggttgtgat ctgagtctag gaagttatga aatttgtttt ttcggctaaa ttttttctca
       61 attctggtta tgaattcaga tgtgacttca ttttttagat cccaaacttc acctaatcga
      121 taatcaaatg ctcttgaatc atttttttca agatttgctg ttacagcacc aataggaaag
      181 tacgattgtt tgcccagcac tacaacatga ggcgtggcac cgcatttatc gcaagctaca
      241 gttgggtcgt caagaacatt gccacattct aaacaagtta tatccattat atatcccgaa
      301 ttataagaat gaatttttat gtggctgaaa attctatcaa aacactaacg cgtacgcaaa
      361 aagtattgta ttgtgttaag agtggttact tcgccacaca gcttaaaccc gccgtcgagc
      421 gggttttgtc gtttctgggc ctggggattc gttgggccta gtctatcccg cagttatcca
      481 ttggctcggc ttctttgacg tttccgcttc tgatttgcgg tacatgatgt ttcctcaatt
      541 tgcacctgct gtatcagcga ggtgagagat aactacaaat gcctcataac ccaaatactt
      601 ggctggagtt ggtccagagc tggtggcgtg gagacacacc gctgggcgca gtgattatgt
      661 cgatcgttat ggctggcttg cgcattgcct attttggcgg tggtggtggc tggaagagaa
      721 aaacgctcga gattttgctc tgtggcgctc tgacgctgac ctttgcatcc gcgcttgagt
      781 atgtcggatg gcctaaatcg ctttctgttg ccattggtgg tggcgttggg ttgatcggtg
      841 tcgatgctat tcgtggggct gcaatgagag taatcggtaa caagtttggt ggctctaagg
      901 agtaatttat gcaggtacta aattcccagc gtaaagcttt cctggatatg gtggcatggt
      961 cagaaggaac ggataacggg cgacaaccga cacgtaacca cggttatgac gttattgtcg
     1021 gtggcgaact cttcactgat tactccgatc accctcgcaa acttgtcacg ctaaacccga
     1081 aactcaaatc aacagctgca ggccgttatc aacttctttc acgttggtgg gatgcttacc
     1141 gcaagcagct tgggctgaaa gacttctctc ccagaagcca ggactcagtg gcattacagc
     1201 agattaaaga gcgtggcgct ttaccgatga ttgaccgcgg cgatattcgt caggcaatcg
     1261 accgttgcag caatatctgg gcttcactgc cgggcgctgg ttatggtcag ttcgagcata
     1321 aggctgacag cctgattgca aaattcaaag aagcgggcgg aacggtcaga gagattgagg
     1381 tatgagcaga gtcaccgcga ttatctccgc tctggttatc tgcatcatcg tctgcctgtc
     1441 atgggctgtt aatcattacc gtgataacgc catgacctac aaagagcagc gcgataaagc
     1501 cgcatccatc atcgctgata tgcagaagcg tcaacgtgat gtagcagaac tcgacgccag
     1561 atacacaaag gagcttgctg atgctaacgc gactatcgaa agtctccgtg ctgatgtttc
     1621 tgctggtcgt aagcgcctgc aagtcgccgc cacctgtgca aagtcaacga ccggagccag
     1681 cagcatgggc gatggagaaa gcccaagact tacagcagat gctgaactca attattaccg
     1741 tctcagaagt ggaatcaaca ggataaccgc gcaggttaac tacctgcagg aatacatcag
     1801 gacgcaatgc ctgaaatgat cgggcgatga aaaccaaaaa aacaggagca atacatgact
     1861 aagctttatc accgcatctc agcttttctc tctgggtgct gggcgtttat cacgtctatt
     1921 tcgttcgtca tctttagttt cggtagcaca gcatgctcgc ttagtcgggg tctgtggcgt
     1981 gctatttcag cactagcgcc gaaattttta cctgaaaagg ttgtttggcg aattgtagag
     2041 cgaatgtgta gtgagagcgt tcgcgagaag attaacgtat ttggacgtca tcctcgaaat
     2101 acaggcgcat tgtgcagttc gttactgtag tcattacaaa gcccatcttt gggtttggtt
     2161 tgtaatttcg ctgctgcaat aatcaccaga aatatggcat tgtaattggt ggtcatatgt
     2221 catacccatc aattacaaag gagcagacat gtcatctaca aaattagtta aaactatcct
     2281 tttccatgga gataataaat atgaagttta tgcaacctcc gagtacgctg acgtagatag
     2341 aatcattcat tacaagttac gctcatgtaa aaacacagat ggttcagatg gcaagtctga
     2401 atctatatgg gttgtatata atcctgctgt tcaactaccg gatgatctga actacaaaaa
     2461 agttagcaac atagctcctt atcttgaaaa gttacaataa acttcctttt ccacatgcta
     2521 aaagcctcgt attcgcgggg ctttttattg tcattacaaa agccactccc tacagagtgg
     2581 ctttgataat ggtttatacc ctacacggga taacttaact gatatccctt ttaacggata
     2641 aacggagcca acaatggcag agaatgtcgg cattatggca gttaaatttg gataaatcgg
     2701 agattagtac atatgccgcc acgaatcccg aaagcctgcc gtgctcgagg ttgccgtaat
     2761 accaccactg acccgtcagg ctattgcgaa agccacaaaa gcgaaggctg gaagcaatac
     2821 aaacctggac aatcccgtca tcagcgcggc tacggttcga agtgggacag tatccgcgcg
     2881 cgtgtcctga agcgtgacaa aggcctgtgt cagttatgtc tgcgttctgg tgtggtgcgt
     2941 gaggcgaaaa ctgttgacca catcatccct aaagcgcatg gcggcactga tgctgacagt
     3001 aatctgcaga gtctgtgctg gccgtgtcat aaggcgaaga cggcccgtga acggttaaag
     3061 tgataataat tctcaactgt ctgaggggag gggcgggtca aatccctgtg acctgacgtc
     3121 ttccggactg cccgccccat cgttttttta tacccgcgaa aaatgaaatt taaccaggag
     3181 tgccgcatat ggctggaacg gcggggcgtt ccgggcgtcg ccccaagcca acggcgcgca
     3241 aggcgctggc cggaaacccc ggcaagcgag ccctgaataa agatgaacct gtttttacgc
     3301 ccatcaaagg tgttgagcca ccggagtggt tcgcagaaga aaatctccct ctcgccacga
     3361 tcatgtggca actgacaacc aaagaactct gcggtcaggg cctgttgtgc gtgactgacc
     3421 tcgcggtgct tgagcggtgg tgcgtggcct atgagttctg gcgacgtgcc gtgaaaaata
     3481 ttgccataca gggcaacacc atcaccggtg caatgggcgg cagggtcaaa aatccggagc
     3541 tgaccgccaa aaaagaacag gagtccgaga tgagcagcac gggggcaatg ctcggactcg
     3601 accccagcag ccgccagcgt ctgattggcc tggcggggca gaagaaagcc actaacccgt
     3661 ttctgaaaat catcgagtca tgagccggaa atcttacccc aacgtaaatg ctgccaatca
     3721 gtatgcccgt gatgttgtgc gcggaaagat tgtggcctgc cagtttgtga ttcaggcctg
     3781 ccagcgccat cttgatgacc tgatggcgga aaaaagtaag tcgtttcgtt accgcttcga
     3841 caaggacctg gctgaacggg ccgccaaatt tattcagctg ttgccgcaca ccaagggtga
     3901 gtgggcattc aagaggatgc ccatcacgct ggagccgtgg cagctatttg tggtctgctg
     3961 tgcgtttggc tgggtcaata aagggtcccg gctgcgccgc ttccgggagg tgtataccga
     4021 aatcccccgt aagaacggca aatcggcaat ctctgccggt gttgccctgt attgttttgc
     4081 ctgtgataac gagtttggcg cggaagtgta ttccggtgcc acgacagaga aacaggcgtg
     4141 ggaagtcttt cgcccggcgc gactgatgtg taaacgcaca cccatgctga cggaagcgtt
     4201 cgggattgag gttaacgcct caaacattaa tcgtccggag gatggcgcgc ggtttgaacc
     4261 gctgatcggt aaccccggtg atggttcatc accccactgt gcggtggtgg atgaatatca
     4321 cgagcacgcc accgatgcgc tttacaccac gatgcttacc gggatggggg cgcgacgtca
     4381 gccactgatg tgggccatta ctactgccgg gtacaacatt gaggggccgt gctacgacaa
     4441 gcgacgggaa gttatcgaga tgctcaacgg gtcggtaccc aacgatgaac tgttcgggat
     4501 catctatacc gttgacgaag gcgatgactg gaccgacccg caggtgctgg aaaaagctaa
     4561 cccgaatatt ggcgtgtcgg tttatcgcga atttttgtta agtcagcagc agcgtgcgaa
     4621 aaataacgcc cgtctggcaa acgtctttaa aacaaaacac ctcaatatct gggtgtcggc
     4681 gcgttcggcg tatttcaacc tggtgagctg gcagagctgc gaggataaat cactgaccct
     4741 tgagcagttc gaggggcagc cgtgcattct ggcctttgac ctggcgcgta agctggatat
     4801 gaacagcatg gcgcgacttt atacccgcga gattgacggt aaaacgcatt actacagtgt
     4861 ggctccgcgc ttctgggtac cgtatgacac ggtgtacagc gtcgagaaaa atgaagatcg
     4921 ccggacagcc gaacgctttc agaaatgggt ggaaatgggc gttctgaccg ttaccgatgg
     4981 tgcggaggtg gattatcgct acatcctcga ggaggccaaa gcggcgaaca aaatcagccc
     5041 ggtcagtgag tcacccatcg accccttcgg ggcgaccggg ttgtcacatg accttgctga
     5101 tgaagacctg aatcccgtca ctatcattca gaactacacc aacatgtccg acccgatgaa
     5161 agagctggaa gcggcaattg aatcggggcg ctttcatcat gatggcaatc ccatcatgac
     5221 caggtgtatc ggcaatgtgg tcggcaaaac cattccgggt aacgatgatg tggtgaaacc
     5281 cgtcaaagag caggcggaaa acaaaattga cggtgcagtt gcgctgatta tggcggttgg
     5341 cagagccatg ctgtacgaga aagaagacac gctgtctgac cacattgagt cctatgggat
     5401 ccgctcgctt taactgaggt aattatgatc atgctgattc tcgcgcctct ggtgggcgtg
     5461 ctgggggcgc ttttgctggc gtatggtgcc tggctgattt atcccccggc ggggtttgtt
     5521 gttgccgggg cgttgtgcct gttctggtcg tggctggtag cgcgatatct cgatcgtaca
     5581 cagctgtctg ttggtggagg taaatagtgt tcttttcggg attatttcaa cgaaaaagtg
     5641 acgcaccggt gaccacgcca gcagagctgg cggatgccat cgggttgtcc tacgacacct
     5701 ataccggaaa gcagatcagc agtcagcggg ccatgcgact gacggcggtt ttttcctgcg
     5761 tcagagtgct ggcagagtcg gtcgggatgt tgccctgcaa tctgtatcac ctgaacggca
     5821 gcctgaagca gagagccacc ggcgaacgtc tgcataaact gatctccacg catcccaatg
     5881 gctatatgac gccgcaggag ttctgggagc tggtggtcac ctgtctgtgc ctgcggggaa
     5941 acttttacgc ctacaaagtg aaagcatttg gcgaagtggc tgaactgctg cccgtcgatc
     6001 ccggctgtgt ggaaccgaag cttaacagta gctgggagcc gatctatcag gtcacattcc
     6061 cggatggctc cacggatgta ctgagccagg aggatatctg gcatgtgcgc acgctgacgc
     6121 tggacggact ggtggggctg aatcccatcg cctatgcccg cgaggcaata tcgctggcgg
     6181 cagcgaccga agagcacggg gccagactgt tcagcaatgg cgcggtgacg tcgggtgtgt
     6241 tgcgtacaga gcagacgctg tcagatcagg cttatgagcg cctgaagaaa gattttgagg
     6301 agcgtcacac cgggcttggc aatgctcacc gcccgatgat ccttgagatg gggctggact
     6361 ggaagtcgat ggcgctgaac gccgaggaca gccagttcct ggaaacccgc aagtttcagc
     6421 ttgaagaaat ctgtcgtctg ttccgggtgc cgttgcacat ggtgcagaac accgatcgcg
     6481 ccaccttcaa caatatcgaa gagctggggc tgggatttat caactattca ctggtgccgt
     6541 atctgacccg catcgaacag cggatcaaca ccggactggt acgaaaaagt aagcagggcg
     6601 tttattacgc caaatttaac gccggggcgt tactgcgcgg ggatatgaag tcccgttttg
     6661 aagcctacgc caccgggatc aactggggaa tttactctcc caatgactgc cgcgacctgg
     6721 aagatatgaa tccgcgtccc ggtggtgatg tctatctcac accgatgaac atgaccacga
     6781 aaccctccga tggcagtaaa gccggtaagc agaaggataa cgccaatgca gacgaaacaa
     6841 cgtcttgatg taccgctgag tctgaaatct gtcagtgact ccggtgagtt tgaagggtat
     6901 ggctccgtct ttggtgtaaa ggacagccac gatgatgtgg tgatgtccgg ggcatttgct
     6961 gcttccctgc gggcgtggag tgacagaaaa gcgttacctg cgctgctctg gcagcaccgc
     7021 atggatgaac ccatcggtgt ttacaccgaa atgaaggaag acgatgtcgg gctttacgtc
     7081 aggggacggt tgcttattga tgatgatccc ctcgcaaaac gcgcacatgc acacatgaag
     7141 gccggttcgt taaccggcct ttctattggg tacgtcctga aagactggga atacgaccgg
     7201 agcaaagaag cctttctgct gaaagaaatc gacctctggg aagtcagcct ggtgacgttc
     7261 ccgtctaacg acgaggcgcg gatcagcgac gtcaagaacg cactggcccg cggggaaatc
     7321 cccgaacaga aaaaaatcga aagagtcctg cgtgatgtcg gactctcccg tacccaggcc
     7381 aaagcattca tggccggggg ctatggcgca ctgtccctgc gcgacgctga ggatgtgggc
     7441 tctgcactga atgcactgaa aaatctgaac ttctaatcag gagaaatacg atggcggttg
     7501 atattaaaga tgtcgaacag gtcgcgcagg agctgcagca gaagtttgac gacttcaaag
     7561 caaagaacga caagcgcgtg gatgcgattg agcaggaaaa aggcaagctt gccgggcagg
     7621 tggaaaccct gaacgggaaa ctcagcgagc tggaaaatct caaaagcgac cttgaaaaag
     7681 agctgcttga gctgaaacgt ccggcaggtg gtgcgcaaaa taaactggcc accgagcata
     7741 aagaggcgtt tgtgggcttc ctgcgtaaag gccgtgaaga cggtctgcgc gatctggagc
     7801 gtaaggcatt gcaggtgggt accgatgaag acggtggcta cgccgtgccg gaagaactgg
     7861 atcgcaacat tcttaacctg ctgaaagatg aagtggtgat gcgtcaggaa gccacggtga
     7921 tcaccgttgg cggttccgac tacaaaaaac tggtgaatct gggcggtacg gcttccggat
     7981 gggtggggga aacggatacg cgatcccaga ctgccacctc cagactggag ctgattgaac
     8041 ctctcatggg ggaaatttac ggcaacccgc aggctaccca gaaaatgctg gacgatgcct
     8101 tcttcaacgt ggaggcctgg atcaacagcg agctggcaac cgaatttgcc gaacaggaag
     8161 aaattgcctt tacctcaggc gatggcacca agaagccgaa agggttcctg gcgtatgaat
     8221 ccactgatga aaccgacaag gtccgggcgt tcggcaaact tcagcatatt gtatccggcg
     8281 aagcgaccgc ggtgaccgca gacgccatta tcaaactgat ttacacgctg cgtaaggcac
     8341 accgcactgg cgcgaagttc atgatgaaca acaacagcct gtttgccatc cgtctgctga
     8401 aagacaccga gggtaactat ctgtggcgtc ctgggctgga actggggcag ccatcctctc
     8461 tggcgggtta cggtatcgct gaaaacgagc agatgccgga tattgccgcg gatgcgaaag
     8521 ccattgcatt tggtaacttc aaacggggtt acaccatcgt tgaccgtatc ggtacccgca
     8581 ttcttcgcga tccgtacacc aataaaccgt ttgtcggttt ttataccacc aagcgcaccg
     8641 gcggcatgct ggtcgattcg caggccatca aactgctgaa gattgcagcg gcgtaatcac
     8701 tcaggggcgc ggaaccgcgc cccctgttct gacgggtgaa gaatcatgat cctgaaacaa
     8761 gatctgaaat ggtcaccgga cggtatgcgt gttgaggtca ttcgggccgg tgagtatgac
     8821 gacggggcgc ttcctgcccg ggtgcaggag attgcacttc aggccgggtt agcagagcgc
     8881 ggaaccagtg caaaaagcag taaagcgaca aaagagaaaa aagccacgac cagtaaagag
     8941 ggctgagtat gcttctgaca atggaagaga ttaaagccca actccggctg gatgaggatt
     9001 tcgatgctga tgaccgccat ctgcaactgc tggcctgtgc ggcgcaaaag cggacggaaa
     9061 cgtatctgaa ccggaagctc tatgctccgg atgaaaccat tccggacagc gatccggacg
     9121 ggctgcacct gccggatgat attcgtctgg ggatgctgat gcttatcagc catttttacg
     9181 aaaatcgctc gtcggttaca gacgttgaga aaatggagtt gccaatgagc tttaactggc
     9241 ttgtcggccc gtacaggtat ttcccgcaat gaaaattcgt caggcgcaga ccagcgcaac
     9301 ctacattctg ccggaccccg gtgaactgaa taaacgcgtc ctgatccgcc agcgggtgga
     9361 tatgcccgcg gataactttg gcgtggagcc tcaatacccg gttacgttcc ggacatgggc
     9421 gaaggttatc cagaccagtg ccaccacctg gcaggaaacc gcgcagaccg gggacgccat
     9481 cacccattac atcaccattc gttaccgccg ggggatcacc gctgattatg aggtggtctg
     9541 cggtgacagt gtgtaccggg tgaaacgtca gcgcgatctg aacggggcgc ggcgctttct
     9601 gctgctggag tgtacggagc tgggcgaatg taggcagagt cacggaggca acaatgacga
     9661 cttccttttt gcacgttgat tttcagcagc ccgcggagat gcgctttaac cgcgcccgtg
     9721 tccggcgggc gtttgtcacg attggtcagc gtcatatgcg tgatgcccgt cggctggtga
     9781 tgcgccgtgc acggtcggca ccgggtgaaa accccggtta tcagaccgga cgcctggctc
     9841 gttcaattgg ttacatggta cccagagcca gtaaacatcg ccctggtttt atggcacgta
     9901 tagcccctaa ccagcgtaat ggagagggaa accgccgtat caccggtgat ttttatccgg
     9961 cttttttgtt ctatggcgtg aggcgagggg caaagcgtcg tcgcggccat catcgtggtg
    10021 catccggtgg cagcggctgg cgactggctc cacgtaataa cttcatggtg gaaactcttg
    10081 aaaagaaccg cagctggaca cgctattttc tggcgcggga attgcgtaaa tcactgaagc
    10141 cggagcgacg acacagatga aactgacgcc tgttattgct gcactgcgtg cccgttgtcc
    10201 gtattttgaa aaccgggttg caggcgcggc ccagttcaaa aatctgccgg aggtcggaaa
    10261 gctgaaactc ccggcggcat atgttgtacc gggtgatgat tctccgggag aaaacaaaag
    10321 ccagaccgac tactggcagg agctgaaaga gggtttctcc gtggttgtca tactgagtaa
    10381 cgggcgtgat gagcgcggtc agtttgcctc gtatgatgtg gtggacgatg tccggcagat
    10441 gctctttaag gctctgctgg gctggaaccc ggaggcgtgc ggtaacccga ttacctatga
    10501 cggcggcacg ctgctggatc tgaatcgtca tgagctgatt tatcagttcg atttttcggt
    10561 catcagcgag ctgactgaag acgatacccg ccagcaggat gacctgaaca gtctggatga
    10621 actgcgaacg ctggcgattg atgttgatta tctcgatccc ggtaacgggc ctgacggcga
    10681 tatcgaacat cacaccgaaa taacccttcc ttcctgagaa tcttcatgtt tgtgaaacct
    10741 gttaaagggc ggtcagttcc tgaccctgcc cgcggtgacc ttttgcccgc cgaggggcga
    10801 aatgttgatg agaacaacta ctggctgcgc cgtgaagcag cgggtgatat ccggcgcgtg
    10861 aatgaaaagg tgaataccga tgacgataag ctttaacacc attccgtcga atacgctggt
    10921 tccgattttt tatgcggaaa tggataacca ggcggcgaat actgcacagg acagcggagc
    10981 atcgctgctg attggtcatg ccaataacgg tgcagagatt gttgccaaca gtctggtgct
    11041 gatgccatcg gcagactatg cacgccagat ttgtggtgcg ggaagtcagc tggcgcgtat
    11101 ggtcgaggct tatcgccaga ccgacccgtt tggtgaactg tatgtgattg ccgttcctga
    11161 atccacgggc gcggcggcaa cagttacgct gacggtgacc ggcgcggcaa ccgaaaccgg
    11221 cacggtgaat gtttatgtgg gacgtacccg cgtgcaggct ccggtgacca acggcgataa
    11281 cgtcacgacg attgccagca gtatcaaaga tgccatcaat gccgttccgg ccctgccgtt
    11341 tacggcttca tcttcggcag gcgtggtcac actgaccgcg cgtcataagg ggctttgcgg
    11401 gaatgaaatt cctgtcagcc tcaattacta cggctttggt gggggcgaag tgctgccagc
    11461 gggcgtacag attgccgtgg cgacgggtac cgccggtacg ggtgctccgg ttctcaccgg
    11521 cgcggtggct gcaatggcgg atgagccgtt tgattatatc ggcctgccgt tcaacgacac
    11581 ggcctccgtt aacacgctgg tgaccgagat gaacgatacc agcggtcgct ggagctatgc
    11641 gcgtcagctg tatggtcatg tgtatacggc aaagatcggc acgctgtcag aactggtgac
    11701 cgcaggtgac cagtttaacc agcagcacat taccctggcg gggtacgaaa aagacaccca
    11761 gacgcctgcc gacgagctgg cggcaagccg taccgcccgc gcagcggtgt ttatccgcaa
    11821 cgatccggca cgtcccacgc agaccggtga gctggtgggt atgctgtctg cgccgaaggg
    11881 gaaacggttc acgatgaccg aacaacagac cctgctgtct catggcgtgg caacggcgta
    11941 tgtcgaaagc ggggtactgc gcattcagcg tgatgtcacc acgtacagga aaaacgctta
    12001 cggggttgcg gataacagct acctcgacag cgagacgctg cataccagtg cgtatgtact
    12061 gcgcaaactg aaatccgtca ttaccagtaa gtacgggcgt cacaagcttg ccagcgacgg
    12121 tacccgcttt ggtcccggtc aggcgattgt caccccggcg gtgatcaaag gggaactgct
    12181 ggcaacctac cgtcagcttg agcgtgcggg gatcgtggaa aactacgaac tttttaagca
    12241 gtacctggtt gtggagcgtg atgccagcga tccgaaccgc ctgaacacgc tgttcccgcc
    12301 tgactatgtt aaccagttgc gtgtctttgc cgtggttaac cagttccgtc ttcagtattc
    12361 agaggagtct gcataatggc ccgtatcggg ggaacctgtt atttcaaaat tgacggtcag
    12421 cagctatcgc tgaccggcgg cattgaggtg cccatgaaca ggatggttaa tgatgacatc
    12481 atcggcctgg acggttcagt ggaccgcaag gaaactcacc gtgcgcctta tgtcaaaggg
    12541 accttcaagg tgccgaagaa ttttccggtg ggcaaaatca cctcgtctga tgagatgaca
    12601 atcactgccg agctggcgaa cggtcaggtc tatgtactgt cgtctgcttg gctgcacggc
    12661 gaagcgaacc ataatgccga agaaggcacg gtcgatcttg agttccacgg tgaagaaggg
    12721 gattaccagt aatgaaagag cttgagttaa agaaaccgat taccgctcat ggcgagacac
    12781 tctccgtact ggagtttgat gagcccaccg ggaaagatgt ccgcgagctg gggtatccct
    12841 accagatgaa tcaggatgag tccgtcagac ttctggcgca tgtggtatcg aaatatattg
    12901 tgcggctggc gaaagtgccg caaagctctg tcgaccagat gtctccggca gacctgaatg
    12961 cagcggcgtg gcttgtggct ggttttttcc tccaggcctg acggctgaat accttactga
    13021 tcgcttcttt gactgcgcca gctactggcg cattaatcct ttcgaattgc tgaatatgcc
    13081 gatcagtgaa attcccttgc tggtcagtca ggcaaacagg atagagcagg agaaacgcac
    13141 acatggctga atttgagctt aaggcgttga tcaccggtgt cgacaggctt tctcccgcgc
    13201 tgtcgaaaat gcaaaagaaa atacggggat ttaaacgcca ggcggaagaa gcgtcacagg
    13261 gtgggctggc gcttggtggc ggactggcag cgggtctgac gctttccctg aaatcttatg
    13321 ccgatcagga aaacgccgcc accgggctga aagtcgccat gatggatgcg aatggcgagg
    13381 ttggaaagcg ctttcaggac atcaataaac tggctattgg cctgggtaac cagcttcccg
    13441 gtacaacggc tgatttccag aacatgatgc agatgctggt gcgtcagggg atcccggcag
    13501 aaaacattct tggcggtgtg ggtaaagcga cagcttatct tgcggtacaa ctgaaaaaaa
    13561 caccggaagc ggctgctgag tttgctgcaa agatgcagga tgctaccgga acggcgtcag
    13621 aagacatgat ggggctgttc gacactatcc agaaggcgtt ttatctgggc gttgacgata
    13681 ccaacatgtt gtccttcttc actaaaacca gttctgttct gaagatggtg aacaaggacg
    13741 gtcttcaggc tgcacagagc cttgccccca tcagcgtcat gatggatcag atggggatga
    13801 acggggagtc ggcaggtaat gccctgcgaa aagttatcca gtccggatta agcgttaaga
    13861 aaatcaggga cgtcaataaa atcatggccc gccagaaact cggggtacag ctcgatttta
    13921 ctgacggcaa aggaagtttt ggcggtcttg ataacatgtt caggcaactg gcaaagctgc
    13981 gaaaactgac cgacgttaag cggacaggtg tacttaaggc aatatttggt gatgatgccg
    14041 aaacccttca ggtggtcaat gcactaatcg ataaaggaaa ggatggctac gatcagatcc
    14101 agcagaagat gaataaacag gccagcctga ataaacgtgt tcaggcccag cttggtacgc
    14161 tgtccaacct gtgggaggca atgacgggga ccgcaactaa cggccttgcg gctattggcg
    14221 gcgcattttc tggtgacgcc aaaaatatca cgcaatggct gggggagttg ggggaaaaat
    14281 tcacgaagtt tgcggatgaa aatccccggg ttattcgcgg cgtcgtcggg cttgctgccg
    14341 gtcttgcgat tctgaaactg ggattgatgg gcgttggcag tgccatcagt attgtcagca
    14401 ggctcatgtc gatgacgccg attggcatga ttgcgacggc gatagccctg gctgcgggat
    14461 taattatcac taactgggat gttgtcggac cttattttaa gaaactctgg gaaaccattg
    14521 gtccttattt tgagactggc tgggaactcc ttaagaaagt ttttgcctgg tcgccgctgg
    14581 ggatggtgat caataactgg gggccggttg ttaagtggtt tcaggatatg tgggacaagc
    14641 tgaagccaat tattgagtgg tttaccgaca gttccggtga cacggtcgat gccattaact
    14701 cggcgcagtg gggggcgggt gcttatgatg cttatgggac gggaataccg gcacggggat
    14761 acacacctta tccggcggta gatccggctc agtcaaacaa cgcctccgat gccacaggcc
    14821 cgaatccctt catgattaac aaagcttctg cgccaaaagt tgatggtgag atcaaggtct
    14881 cttttgtgaa ttcgcctccg ggtatgcggg ttatggaaac gcgatccagc ggttttgatg
    14941 tcagccatga tgttggctat acgcgctttg gcaggtaatg aaaaattaat ctgttaatga
    15001 gtcccactcc ggtgggattt tttatgtacg gagtttatat gacgtggaaa gacagacttc
    15061 aggacgcgtc atttcgcggt gtgccgttta aggttgaaga agaaagtgcg ggaaccggtc
    15121 gtcgtgtgga aacgcacgaa tacccgaacc gcgacaaacc ctataccgaa gacctgggga
    15181 aaatcacttt ccgcccgtcc atcacagctt atgtggtggg agatgactgc tttgaccagc
    15241 gcgatcgcct gattgacgcg ctgaataaac ccggtcccgg cacgcttgtc catccgactt
    15301 acggtgagct gaaagtctgt gttgacgggg aagttcgggt cagcacatcg aagagtgaag
    15361 ggcgtattgt ccgctttgac ctgaagtttg tcgaagcggg agaactctct taccccactt
    15421 caggtgcggc gacggcgcag acgctgatgt catcctgttc tgcactggat gactgcatca
    15481 gtgacagctt cagtggtttc agtatcgatg gcgtggcaga ttttgtgcag aacgacgtcg
    15541 tcggtaatgc cggcacaatg ctggggtatg tttctgatgc gatgaaagtg gtggattctg
    15601 ccgtatcgga tgccgccagg ctgttgcagg gggatatctc ggtacttctg ccgccgccat
    15661 cgtcaggcaa aaatttcgtt gagcaggtgc agaaaatgtg gcgtaccggg aaacgccttt
    15721 atggtaacgc cagcgacctg gtcaccatga tcaaaacgct ttccggtgtc agcctcggca
    15781 gcgatctgca accgcgcggt gtctggaaaa cggacagtaa aaccaccgcc acggcgacgc
    15841 agcagcgtaa cgtggttgcc agcatccttc gtacgaccgc aatcagcgaa gcggcgtatg
    15901 ccgtcacccg attgcctgcg ccaacaactt ccgcggtgat gcagaatgcc gcagtggggc
    15961 aggcaacaac acccgcgcag agcactggct ggccttccgt cacgcatccg gcactgaaca
    16021 atgcaccggc ggtgaaaaac acggttgacc tgccgacgtg ggaagaactg actgacattc
    16081 gcgacacact gaatacggca attgataagg agttgtcccg tacaaccagt gatgcgctgt
    16141 ttctggcgct gcgccgggtg aaagcagatc tgaatgcgga tatcaacacg cgccttgaac
    16201 agtctgcacg gatcattcag cgcacgccgg atgaggtttt acccgcgctg gtgctggcgg
    16261 cgacctggtt tgataacgcg gcgcgtgacg cggacattat ccggcgtaat gccattacac
    16321 atcccggctt tgtgccggtg atccctctga aggtgccagt gcaatgaacg acaatgtcac
    16381 gctacgggta aatggccggg agtggaatgg ctggacatcg gtgcgcatcg gtgccggtat
    16441 tgaacggctg gcgcgggatt tcagtgtgga gatcactcgc cagtggccgg gagatgaggg
    16501 tatcaccacg cttcagccgc gcattaaaaa cggttcaaaa gtggaagtgc tgattggtga
    16561 tgagctggtg atcaccggct gggtggaggc gactcccgtt cgttacgatg cccgttcggt
    16621 cagcaccggt attgccggac gtagtctgac ggctgacctg attgactgtg cagccgaacc
    16681 gacacagttt aacggacgct cgctggtgca gattgcgcag gcgcttgctg cgcctttcgg
    16741 cattgaggtg gtgaacagcg gtgcgccgtc gggtgttatt cctgatgttc agcctgatca
    16801 cggtgaaacg gtgattgagg tgatcaacaa aatactcggt cagcagcagg cgctggctta
    16861 cgacgacccg cacggcaggc tggtgattgg cggtattggc tcaacgcggg cacataccgc
    16921 gctggtactt ggggaaaaca tcctttcctg tgatacggag aagagtatcc gggagcggtt
    16981 ttctgtttac caggtggcgg ggcagcgtgc cggaaacgac gatgatttcg gtgaggccac
    17041 caccaccgcg ctgcgggcac gcacagagga cgcatttatt gcccgttacc gtccgatgta
    17101 tatcaggcag acagggcagg ccacgggggc aggttgtatt gcgcgtgctg actttgaagc
    17161 ccgacaacgg gcggcgcgga cggatgaaac cacctatgtg gtgcagggct ggcgacaggg
    17221 taacggtacg ctgtggcagc ccaaccagcg ggtgattgtc ttcgatccgg tctgtggttt
    17281 cgacaatacc gaactgcttg tctcggaagt cacgtttact caggaccaga acggcaccca
    17341 gacggaaatc cgtgtcggcc cacctgatgc ttatctgcct gaacccgaag cccccggcgc
    17401 gcggaaaaag aaaaaagcca gagtacagga ggacccgttc tgatgaggac gattgaaacc
    17461 atgcagcgac aactcctcgg cctgattggg cgggccgtgg tgaaaagcat cagtgccgcc
    17521 acgaaatgtc agaccgtgga tgtgtccctg attgccggtg aacccaaagc cggggttgaa
    17581 catcttgaac cctacggttt taccgcaagg gcaaacagcg gtgcggaagc ggtggtgttg
    17641 tttccggatg gcgaccgttc tcatgcggtg gttgttacgg tgtcggaccg gcgctaccgc
    17701 ctgaaagggc tgcagacggg tgaggtggct gtctatgacg atcaggggca gtccgtgacg
    17761 ctgacccggg aggggatcgt ggtggacggt gcaggtaaaa cgatcacgtt tcgcaatgcg
    17821 cccagagcac gttttgaaat ggacctggaa gtgaccggac aggtgaaaga cctgtgcgac
    17881 tccggcggca ccaccatgtc agcgatgcgg cttgcctata acgggcatcg tcacagagag
    17941 aacggtcagg gcagtaacac cgacaaacct gataaagcga tggaggcatg atggaactgt
    18001 ggctgacggt gaacggtaaa cgcacctgcg ccagcgcacc gctggatccg ctgacccgcg
    18061 ccgtggtgat ttccctgttt acctggcggc gggcggagcc tgatgacaac gccgacgtcc
    18121 cgatgggatg gtggggggat acctggcctg cggtacagaa tgaccgttac ggctcccgac
    18181 tgtggctgct tcagcgcagc aaactgacca atcagctggt gcagacggta agggggtata
    18241 tccgcgaatg cctgcaatgg atgactgatg acggcgtggt gtcccgtatt gatctggata
    18301 tccgccgcac cgggattaat gaactgggta acagtatcac tctctggcgt cgtgacggac
    18361 cggtaatgat ttcttttgat gatctgtgga gtgcgataac gcatggcgga cagtgaattt
    18421 cagcgcccga cgctggcaga aaatatcagt atgctccgta acgatttatt tgccaggctg
    18481 gacgtcagcg acacgctccg gcgcatggat gaagacgtgc gggcaaaggt gtatgcggcg
    18541 gcgctgcata cggtttacgg gtacatcgat tatctggcaa tgaatatgct gcctgacctg
    18601 tgcgatgagt cctggctggc gcgacatgct gcgatgaaac ggtgtccgcg caagggggcc
    18661 acggctgcca gcgggtatat gcgctgggaa ggtgtcagcg atggcctgaa ggtgactgcc
    18721 gggagtgtta ttcagcgcga tgacctggtt cagtacacgg caactgccga tgcaaccagc
    18781 tccggtggtg tcctgcgtgt gccgatcact tgctcaacta caggcgcggt cggtaacgct
    18841 gacgacggta cggcattaat cctggtcacg ccggtgaatg gtctgccgtc ttccggtgtt
    18901 gcagataccc tgactggagg attcgatact gaagatctgg aaacgtggcg cgcccgcgtc
    18961 attgagcggt attactggac gccgcagggc ggggctgacg gggactatgt cgtctgggct
    19021 aaagaagtgc ccggcattac ccgcgcatgg acataccgtc actggatggg aacgggaact
    19081 gtcggtgtga tgattgccag cagtgacctg attaatccca ttccggaaga atcaacggaa
    19141 acggcggcaa gacaacatat cgggccactg gccccggtgg caggctctga tttgtatgta
    19201 ttcaggccgg tggcgcataa agtggatttt catatccgcg tgacgccgga cacaccggaa
    19261 atacgggctg ccatcaccgc cgagttgcgt tcgttcctgc tgcgtgatgg ttatccgcag
    19321 ggagaactga aggtgtcacg tatcagtgaa gcgatttccg gtgcgaacgg ggaatacagc
    19381 catcagttgc ttgcaccggc agacaatatc tccattgcaa aaaatgaact ggcggtactg
    19441 gggacgattt catggacgtg acaaacgatg attacatccg tctgttgtcg gcactgttgc
    19501 cccccggtcc ggcgtggtca gccagcgatc cggcgattgc cggtgcggca ccgtcattaa
    19561 cccgcgttca tcagcgtgcg gatgccctga tgcgggagct ggatccgcgc actaccactg
    19621 aactgataaa ccgctgggag cgtctgtgcg gtctgccgga tgaatgtatt cccgcaggga
    19681 cacagaccct tcgccagcgt cagcaacggc tggatgcgaa ggttaacctg tcgggtggca
    19741 tcaacgagga tttttatctt gcacagcttg ctgccttggg cagaccagat gccaccatca
    19801 cgcgatacga caaaagcacg ttcacctgct catcggcctg tactgacgct gtgaacgcgc
    19861 ctgaatggcg gtattactgg caggtcaaca tgccagccgc caccaacacc acctggatga
    19921 catgtggcga tccctgtgat tccgcactgc gtatctgggg cgacaccgtt gtcgagtgtg
    19981 tgcttaacaa actctgcccg tcgcatacct acgtaatttt taaatatccg gagtaatcca
    20041 tgcatcgtat agacacgaaa accgcgcaga aggataagtt cggcgcgggt aagaacggtt
    20101 ttacccgtgg taacccccag accggcacgc ctgccaccga tctggatgat gactactttg
    20161 acatgttgca ggaggagctt tgcagcgttg tggaggcgtc cggtgccagc ctggagaagg
    20221 ggcggcacga ccagctgctt accgcgcttc gtgcgctgct gttaagccgc aagaatccgt
    20281 ttggtgatat caaatcggat ggcacggtga aaacggctct cgaaaacctt ggtttgggag
    20341 aaggttctcc agctattggc gttccgttct tctggccgtc cgctgcaatg ccaaatactg
    20401 taatcgacag ctggtccagt atggtgtttt tgaagttcaa cggcgcgaaa ttctctgcca
    20461 ctgattatcc tgtgctggcg aaagtgtttc cttcgctggt attacctgaa gcccgcggtg
    20521 atttcattcg tatctgggat gacgggcgag gtgcagatgg tggtcgtgaa ttattaagct
    20581 ggcaggcagc tacaaacttt tctcagtttg ccgggaatat aggcgaaggt gcgggacacg
    20641 caattaactt tcatgatggc atcgccggaa atcagccagg attttcacga tttaatttca
    20701 ccagtaactc tgtgggtgat ggtgtgaatt ttgttgctgt cagaccgcga aatattgcat
    20761 ttaactttct ggtgagggct aaataatgaa acctgttttt gatgaaaatg ggctggctac
    20821 agtgccgggc gatatgcgtt gtttttatta tgatgctgaa acatctgagt atacgggctg
    20881 gtctgatgaa tatattaata ctggcgtaag tatgcccgcc tgttccactg gtattgaccc
    20941 tggcgaaaac attccgggaa aagtggcagt atttacgggt aagggatgga gccatgaaga
    21001 agaccatcgc aatgagactg tttactcaac agaaaatggc gcagctgtta cagtggatta
    21061 tatcggtgcc atcaaagacg gttatgtcac gctttcaccg ttaacgccat acgataaatg
    21121 ggatggtgag aaatgggtga cggataccga ggcacagcat agcgccgcag tagacgcggc
    21181 agaagcacaa cgtcagtcac tgattgatac tgtaatggct tccattagtc tgattcaact
    21241 gaaattgcag gccggacgga aactgacgca ggcagaaaca acccgactta acgccgtgct
    21301 ggattacatt gacgcggtga cggtaacaga taccagcacc gcgccggata tcatctggcc
    21361 tgaactgccg gaggcgtagg ccattcaata tctggcgcac tggaagtatc gaccagctcc
    21421 agtgcgtcca gataatccag ccacaaatta tattgcgcca gttcctcacc tttcagacga
    21481 ccaatagccg ctttaccagg ccattgcctg ctgttcatgt gctcgttggc ttcattaata
    21541 agttttcctt ttttcaactc tgccaatgca ataaggtttt cttttgataa aggtggttgc
    21601 tctgtcaaaa ccggatatcc ctcctgattg ctgactattt tcatgccatt atcctgacca
    21661 tccagtagtg acagccattc atccgtggtt atctcaacag catctgaagg tgctttattt
    21721 aaatcggtaa aaaaaccatt ttctttttgt gaatagaagt atctgtccat ttatcaatct
    21781 ccaaaagcaa tccagtaagc aaaaggattt attccttgct cagtcacaga tgacatcagg
    21841 gaaaattgcg atggtgaaac aggtaa
//
LOCUS       DAZGCS010000080        21643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGCS010000080 DAZGCS010000000
VERSION     DAZGCS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.080"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..307
                     /locus_tag="ACHK01_003309"
     CDS             119..307
                     /locus_tag="ACHK01_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098218.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSI
                     LKRRGDTAALTLLQDTGV"
     gene            317..1516
                     /locus_tag="ACHK01_003310"
     CDS             317..1516
                     /locus_tag="ACHK01_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO8098219.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITT
                     ESQWRSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDIS
                     LLRYMPA"
     gene            complement(1631..1819)
                     /locus_tag="ACHK01_003311"
     CDS             complement(1631..1819)
                     /locus_tag="ACHK01_003311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098220.1"
                     /translation="MAGAHEFRQHGFHARRRAEIISPLAQSETVGHEAADMAAQALGL
                     TYRKLKNQSIPLSTPYAT"
     gene            complement(2137..2985)
                     /locus_tag="ACHK01_003312"
     CDS             complement(2137..2985)
                     /locus_tag="ACHK01_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8098221.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(3025..3303)
                     /gene="yacB"
                     /locus_tag="ACHK01_003313"
     CDS             complement(3025..3303)
                     /gene="yacB"
                     /locus_tag="ACHK01_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO8098222.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(3303..3572)
                     /gene="yacA"
                     /locus_tag="ACHK01_003314"
     CDS             complement(3303..3572)
                     /gene="yacA"
                     /locus_tag="ACHK01_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO8098223.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(4481..5512)
                     /gene="repA"
                     /locus_tag="ACHK01_003315"
     CDS             complement(4481..5512)
                     /gene="repA"
                     /locus_tag="ACHK01_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO8098224.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            6470..6628
                     /locus_tag="ACHK01_003316"
                     /pseudo
     CDS             6470..6628
                     /locus_tag="ACHK01_003316"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            6666..6953
                     /locus_tag="ACHK01_003317"
     CDS             6666..6953
                     /locus_tag="ACHK01_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8098225.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            7395..7928
                     /locus_tag="ACHK01_003318"
     CDS             7395..7928
                     /locus_tag="ACHK01_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO8098226.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            8182..8865
                     /locus_tag="ACHK01_003319"
     CDS             8182..8865
                     /locus_tag="ACHK01_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFO8098227.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            8879..9445
                     /gene="traD"
                     /locus_tag="ACHK01_003320"
     CDS             8879..9445
                     /gene="traD"
                     /locus_tag="ACHK01_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TraD"
                     /protein_id="HFO8098228.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            9713..9967
                     /locus_tag="ACHK01_003321"
     CDS             9713..9967
                     /locus_tag="ACHK01_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO8098229.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            10010..10462
                     /locus_tag="ACHK01_003322"
     CDS             10010..10462
                     /locus_tag="ACHK01_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFO8098230.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIKNSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
     gene            10512..11102
                     /locus_tag="ACHK01_003323"
     CDS             10512..11102
                     /locus_tag="ACHK01_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFO8098231.1"
                     /translation="MKNNNVPLYLWLLWCTFCLYITTPWQYFRYLYTDHISLSYILYF
                     CIVVIICFSPLLFRCVLLIVRSVSKYNFTFFAKKNGSKESSIHLSPYADTRCPESMRK
                     HWDVLLSLISKAINNDETIVMHSHLFTPARINKLIKRLESYGLNFSVITCPRKTSLYE
                     RLSIPFVYLLFQWSLPHLHSDGMTVVIRPLQPPHTN"
     gene            11136..12203
                     /gene="pilL"
                     /locus_tag="ACHK01_003324"
     CDS             11136..12203
                     /gene="pilL"
                     /locus_tag="ACHK01_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFO8098232.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            12203..12640
                     /gene="pilM"
                     /locus_tag="ACHK01_003325"
     CDS             12203..12640
                     /gene="pilM"
                     /locus_tag="ACHK01_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO8098233.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            12654..14336
                     /locus_tag="ACHK01_003326"
     CDS             12654..14336
                     /locus_tag="ACHK01_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO8098234.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            14329..15624
                     /gene="pilO2"
                     /locus_tag="ACHK01_003327"
     CDS             14329..15624
                     /gene="pilO2"
                     /locus_tag="ACHK01_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO8098235.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            15611..16063
                     /gene="pilP"
                     /locus_tag="ACHK01_003328"
     CDS             15611..16063
                     /gene="pilP"
                     /locus_tag="ACHK01_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO8098236.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            16074..17627
                     /locus_tag="ACHK01_003329"
     CDS             16074..17627
                     /locus_tag="ACHK01_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO8098237.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            17640..18737
                     /locus_tag="ACHK01_003330"
     CDS             17640..18737
                     /locus_tag="ACHK01_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO8098238.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            18755..19369
                     /locus_tag="ACHK01_003331"
     CDS             18755..19369
                     /locus_tag="ACHK01_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO8098239.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            19379..19939
                     /locus_tag="ACHK01_003332"
     CDS             19379..19939
                     /locus_tag="ACHK01_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO8098240.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            19924..20580
                     /locus_tag="ACHK01_003333"
     CDS             19924..20580
                     /locus_tag="ACHK01_003333"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO8098241.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            20580..>21643
                     /locus_tag="ACHK01_003334"
     CDS             20580..>21643
                     /locus_tag="ACHK01_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO8098242.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         5763 a         5332 c         5097 g         5451 t
ORIGIN      
        1 aaccacggca gcctggcggc tgcaaattga aaaccgcccc ggttcgtcct gttgttgccg
       61 cacgccccgc agaatatgta tgctctgagg acctgtttta ttaaggactc atccccttat
      121 gttgacccgc aaaagtatcg atacagttct gctctctgtt ggtgctgaga agctctcaca
      181 acgagaatgg gactggatga aaatgctcaa acccatggac ccaccacctg cgatggtgac
      241 gacctccatc ctgaagcgcc gtggcgatac agcagccctt accctgctac aggataccgg
      301 ggtttaatcc atcctcatgt tgtccggttt tactccccgc cctctgaaac gtctgttcac
      361 agccaaccag tgctggacgt ccttcctgga tgcgggcggt ctgcgcgata tcgaggttga
      421 agccgtcacc aaaatgctgg cctgcggcac acggatactg ggtgtaaaag agttcgggtg
      481 tgataacccg gactgtcagc acgtaaagta cctgaccaac tcatgcggca gccgtgcctg
      541 cccgtcctgc ggaaagaagg ccacagacct gtggacagca acacagctga atcgtcttcc
      601 tgactgcgac tgggtacatc tggtcttcac cctgccggac acgctgtggc cggtgttcga
      661 aagcaaccgc tggctgctga atgacgtgtg ccgtctggcg gtggagaatc tgctgtatgc
      721 cgcccgaaaa cgggggctgg aacccggtat cttctgcgcc atccacacgt atggccgtcg
      781 tctcaactgg catccgcatg tacatgtgtc tgtaacctgt ggaggtctga ataagcatgg
      841 tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt tcacggtgga tgtggaatat
      901 gcggcagctg cttctgaaag cgtggtcaga ggggatggca atgccggagt cgttgtcaca
      961 tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa tccggcggaa aatactggca
     1021 tgtgtacatg tcgaagaaga cagccggagg gcggaatacg gcacgctacc tgggtcgtta
     1081 tctgaagaag ccaccaatag cggcttcccg actggctcat tacaacggag gggcaagcct
     1141 gagcttccgt tacctggacc acaaaacggg agaaacggcg acggaaacgc tgacacagcg
     1201 tgagctggtc gcgaggctga aacagcacat cccggagaag ttttttaaga tggtgaggta
     1261 cttcgggttc cttgccaacc gtgtgtgtgg agagaagctg ccgcaggtgt accgtgcact
     1321 ggggatggat aaaccggaac cagtggcgaa agtgtgctat gcacaaatgg tgaaacagtt
     1381 cctgagtcgt gacccgttcg aatgcgtgct gtgtggctgc cggatggtat accgccgggc
     1441 cattgcgggg ctgaatgtat cagggctgaa gaaaaacgcg cgggatatca gtctactgag
     1501 gtatatgccg gcctgacaca ggctaagtgt gcccgggaat cggtgaaagt ataaaaaaca
     1561 gcctgttacg gtataaaaat cgaccataca gagagggaaa ccaccggagt ggtgggaaaa
     1621 gcagaaaggc tcacgtggca taaggcgtgg acaatggtat cgactggttt ttcaatttcc
     1681 tatacgtcaa gcccagcgcc tgagccgcca tatcggccgc ttcgtgcccg accgtctccg
     1741 actgcgccaa cggactgatg atctccgcac gacggcgcgc atggaaaccg tgctgtctga
     1801 actcgtgtgc gcctgccatg cacgaaaggg gaatgtttcc tgcccgttga tcgcgtcact
     1861 acagggcgaa gcaggcctgg caaggtcagc tatgccttag cgtgctttat tttccgtttt
     1921 ctgaggtgcc cccagccaga taatcctttt caagtttttc agccgtgtag cacctgccgc
     1981 ctaaaaaaag ccgcatggcc tccctggttt tgtctctcac atatcatccc tcattaatgc
     2041 taatgtacac atacattagc attaacatgc tgacagcaac aagcggcgga tttacgtctg
     2101 gataacgtaa atgattgcgg tatacaggcc aggcggtcag attttcagcc gtgcctgttc
     2161 ctgcagcagt tccagatgat atgcagcaat agcgtttacc acccctgccg tcatccgtgc
     2221 gtcggcgatc gctgagtgtg cctgcccttc ccaggtcagt ccggcctggc tggcagcaca
     2281 ggccagtgaa atcgtgccat agcggttggt ggccccataa tatcctgcag ccagctccat
     2341 cgcacaatat accgtcagtt cttccagcca gtcagccgga tcgctatgtg cggcagcagt
     2401 ctgtttcaga atgcggatgt cgaaccgggc attaaagata attactggtc ggtccccgat
     2461 tgcatgacgc agttgccgcg ccacatctgt ccatgatggt gcgccgcaga gagcctgctc
     2521 gctgatacca tgcacagcgg cggcctgcgc cccgatggcg accgtgggct tcagcctggt
     2581 ttcaaatacc acctgaccgg ccgcatcggt caggccgatt tccagggctt ccgcggtatt
     2641 atccagtcct gtcgtttcag tatccagaaa gagcggtgcc agactcagcc agtcatatgc
     2701 ctgccgtgcc atccggccac tggtgctgtt aagtctggat aaaacggaaa gtcgctggcc
     2761 agccagctgt tgtttactcg tcagagggcg tttctcacgc atcggaacac agtctgcaat
     2821 tcggtataca gcgaacctgc caccataagt gtttttatac cacttaaccg gctcagcacc
     2881 gggggctggt ttcattctga attcatcgcg caaacgtcct ttcgagaaac acctgtcttc
     2941 ggcaatcgcc tgcgcacgtc tggtggccag ctcatcatta ttcatggtta ttgcatctcc
     3001 tttccttata tccggacagc ccctctatgg ccacttctgt gcagtatgca acacgcgcag
     3061 gatatacact tttccccact ggctgtcgac ctcataaacc agaacaaaat gcgggtgaat
     3121 aaccagttcc cgggtacctt ccacacgacc gttgcggcct ttatacggct gcccggccag
     3181 gctgctggct gaataaccaa tccgttcgtc cagttcaatg gcagccatca ggttctgctc
     3241 cgcaatgtac tcacgaatgc gcttcctgtc ctggctggcc agcatggtcc agaatatctc
     3301 catcatttac cggcaacctt cctgctcatt tcatcccgcc atgctgcagc agaggattcg
     3361 accatgtcat ggggaagcac atcgccgtgc tcgagttgct gacgtcctgc cgcaacctgg
     3421 tcacggaacc aggtattatg ctgccgttca acggtctggc gcataaaatc ccggattaac
     3481 tgagagccat tacggtccat gctttttgca gcggccataa aagcatcctt cagttcagcg
     3541 tcgattctta aactcatatt aacctgtgcc atttatcacc tcgtcattac tacgtatata
     3601 cacagtatat aacgattcag atatcgtcac caggatatgc cgcgccagcg gcatggaagg
     3661 cggcacgctg tggttacatg tgataccgga gtaaaaccgc cgaagcccgg cgtcagccgg
     3721 tactgattga tagatttcac cttacccatc cccagccctg ctaagccata cccgctttca
     3781 gccatgagag agtttctgtg cgcggtcgga gtggtcccga cgagggttta cccgaagtcg
     3841 gggcgtatct ccgcgttagc gggccgtcag ggccgcttac gagcgtgtac tgagaacatc
     3901 cagccagaag actgacagcg atgataatgt agttacaaca ttcataatta aaagcgatgt
     3961 tgttcccgcc ctttgggcgg gggcggcctc gcttttcagt tatgaggggt gggcacggtg
     4021 ctgcgtttgt gtggtgtttt tattcctgat gtctttttgt tgtggtttca gaacggtgag
     4081 gttcggggcc cgcgcgttag cgtgggaggt gcttttagat tctgagcggc gcggccttgg
     4141 ccgctgcaag cgaaggcctc tttgtatttg ttcacagtgg tctcagagat aaagattttc
     4201 cctgcggcga agcaacgcag gggattaaat aatggggtta agactatatt gcaggtttgc
     4261 tgtttcaggt ttgcagcttc ttaaagttct ttgtttgcgg atttatgtgg ataactgaaa
     4321 caacgtggcc atgttctgat gactgatgcg tggttttatg ggcagatatg cccctctgag
     4381 gggatttaag gtgattactg cggattttgg gctgagcgga accagcgccg aaagccccgg
     4441 aggggctggt gatgattcag tgctgaggga atggtgctgt ctagtgcggt ggcggggcag
     4501 ccagcgtcag ttcaagctga cgcaactccc tgatggccat tcgcttcaga tggtcatcgg
     4561 aacaaaaata ggcttcatcc ggaggcagac gcttcctgag atactctgcc atctcatgaa
     4621 tctgctggtc ggcaggcagt ttcttcaggc gtctggcgcg cttactggct gctgctttcg
     4681 cacgacggtg tttcagcgca tcctgactgc gctgcagata ccagcgtttt ctggccgcat
     4741 gtacggagat atcttcatcc tcacgcagaa caccttcccg aatgagctgc tggcggattt
     4801 cactttcacg cagtcgtttc tgctgctgtt cgtgaagttt taccatgtcg acgcccagca
     4861 tctgccagcc tgccggtgtt atccagacgt aacgtggcag acgctggcgg gtttcacggt
     4921 cccacagggt ttcctctgaa acacccagca caccaaaacg tacctgttcg gccagcaaac
     4981 gggaaaggcg ggagaccgtc acttccagtt ccggaataac cttccccttg ctgtctttcg
     5041 ggctgatttc tctggccaga cgggaaacac tcatgcctac ggtatgtgtg cccgcatcac
     5101 tgaagctgac cagaaccggc cagacggcat cgaggaggcg ttgtttttcc ggacggaaag
     5161 catatttgcg accgacatac gttcgtttct gcacgtaaaa aggatgcaga gacatgtgca
     5221 tgcgcaggct ctgctcgccg gtcaccgggt cctttttcac cagacggttg taggcgtgcc
     5281 cgagctgccc gctcagcgcc ttatagcgct caggacgata ccaggacgga ttttcgcatt
     5341 tgggtttggt ggagtgctca ccacgacgac gcttcgtgcg cttcctttcc ggttcaacca
     5401 ggaaagtggt cgcccttccc gcttcatagt cttcccagaa acggatctgc tcttcgggtg
     5461 ctaactgact ccagtgtact gcgttatatg gagtattctt cagacctgcc acaagctgtc
     5521 gcttatggcg gagcgattgt gccgtgtatt tatacactgc acagggttga atctctggta
     5581 cggtttcatt atactttacc cgtattctgt gaggccccca ctatctttcg tcgttccgcc
     5641 aagttcgtaa gaaagatagt gggggttttc cttttatggc ttccggaaca tcttcagtct
     5701 tctgacttac tgcccgtaca tgccatctct cctgaatgcc ttatggcgca ttctgccgtc
     5761 cggctttcgc cgctttgctc cacagagtgc caccctctgc acagctaata acagccgaaa
     5821 tatacccgct ttacgttaat ttgaaaagca ttacaacgat ctcttctctt ccagtgcctc
     5881 ctcactgata cctctaataa gttaatcttg acctgcgtca aaaaaataaa atctattttt
     5941 aaaagcagat ttttctgctt tagatctttc ttttggatta aaagatcgac ttttatccta
     6001 ctcacgatca tttatcttac aaccgatcta cctaacagaa taatattctg ttttaatgca
     6061 attaaacgat ctaaataacc tttaaaaaat cattttgtag atcattcgat cgtaaataga
     6121 atgattctta gatgataaaa ccatttgtta cacaacccgc caattgtgta acaacagcct
     6181 ctacaagagc atatttgttc atatgttcgt cagaactgcg gattgtctgg acgagggtca
     6241 gacaccgttt cgtgcatatg gaccagcatg ttcgaacgcc acaaagcaga agcatcatgc
     6301 ctggatcgct tcggcgcgga gcgttaagtt ttgacgcttt tttgtatgtt ttttaagctg
     6361 ttagttgact cggtgcgtta tactaagtac cctttactgg tcacaggcaa aactgtgatc
     6421 gggcgtagga acccgcgtag tcatcgtcgg cgcagaagtt aatttcttca tttggcgtcg
     6481 ttttttgcca taccataaag acaggaactt gtatttcttg tcgtcatcta tggtagctca
     6541 ggcggggcag gcattagcct ggccggtttc cgatgacgcc ggtattccta cccccgtctg
     6601 ggctatcacc aatgagcgta ggaactcggt gatagcgttt ttagttgtca tcggagacct
     6661 ttgctatgtc acctcaactg atacagaaac tcccggccat cactctgttg gaaggcatgt
     6721 ttcccgagct atcaacaaac cagcttaaag tctgcgtttt ttacgcaatg ggtgttcctt
     6781 acgacgccat tgctcagaac tgtcggcttt ctcccgaaac ggtacgaacc tacctgaagc
     6841 gaagcctgaa gaatttaaat ctggaaggat atgatgcact tcgctctgct gtgctgatgc
     6901 gaactttcgt tttcatgatt agcaatacag ctaaagaaaa cgaaaaaatg tgaacaggtg
     6961 tccccattaa aggggacact ttcatggaaa ttgcattttg cactttacag gcgatcccaa
     7021 attgataaac ataaacacca agcacaagtt actttaataa agcaaatcac ttcagacggt
     7081 gtcccagtta tttgtatctg gctcatgccc gatatatggc gaccttactc tgaactgacg
     7141 tgctgatgtt ttatttcgcg tatcacaata ctgtgccact gtattgctga tattagtgct
     7201 tagccgttct ttatctgtga gttcgccaaa tcaccgatac agaacgtact agagtctgct
     7261 ggttatatgc ctgtcagaac tgcagtttat ccagatatgg gctggacact gcttcagaca
     7321 tatgatttag catattcgcc tgacatcgca aaatcgtcca tcatggatcg gttttgcgcg
     7381 gagcgttgaa cttcgtgaat atagaacacc tgaataaccg gaactggtat cttgcgcaat
     7441 ataatacagc cggcaaaaac agagaatctt tattctcatg gctgaatgag caaaacgtcg
     7501 taccatggac tccattaata acaagaaaga taagacgggc agactccagg tgctgttacc
     7561 gtgaaagaat attcgctatt tttccagggt acttttttat tctggctaat tttgacattc
     7621 acccagtatc agcattacgt cgacatagcg ccttcattga tttcgtgaag tttggcggtg
     7681 aaataaaacc cgtcaataaa gatattgttg atggattaat gaagatttac ccggaccctg
     7741 tgctaaatcc gggagccagg gaagagctga atgcggcttc cagcatatgg ctgacaaaag
     7801 cacaatatca gtatctcctc agaatggaaa atactctcca gcctgagtcg aggatatccc
     7861 tgctcctgga actggtatca aatgccgaac atcacggctt tatggagcgc cttgtaaaca
     7921 tcccatagcc agtatttccc ggccttcggg ccgggctttt ttcaattcaa gagaatattc
     7981 cggagtgaag cctgcacgaa gccatcccca ccatggactc cccctgtctt ttgatggaac
     8041 cgtgtatttc ttaatggcga aagaaataaa aggcttcacc ccagagtatt gattcgtttt
     8101 atcaaaggcc ggcccgtgcc atcacgtgtg gcggagagaa cggataccgg tcttcttttc
     8161 atctcacagt aggacattcg catgaccacg acagaacatc ccgtgcgcca gttgctctgg
     8221 tgtgcactga atgccctgaa aacggcacag gaaaaccaca atataaccag tgaaaccgga
     8281 ttacggcatt acctgcttga atggctttct ggtgccagca gacatcctga gttccgtagt
     8341 ctccctggcg aaatcatggc gttaaaagca ttgaccgaaa aagatcgcaa tattcccatc
     8401 atcggaacgc tgaatacgct ctttctcagc tctgccactg tcggggaatg tccgttgttt
     8461 cgttttcggg ctgcactggg taaattacag aaagcaggct ggcgcacata tgtctgccca
     8521 tggcctgaga gggtgttcaa tgaatccatt gaacgggcct gttccgggaa acgtcatctg
     8581 ctacaactaa gtcgcaccga agagtgtttt ctaccgacag gcgatatgag ggcaccagtt
     8641 acgttgcagc tgatcactcc actacaccgc aaaactatgc agcttctgga agcggcagag
     8701 ttaatattct ccgacgaagg atttcaggtg gtgagaggat acgaacatca attcgggatc
     8761 gaacgcagag aaaccctctt tcacacctta tttatcgggc tgcccagcct gccagaagat
     8821 gtctggggtg caacacagga gcagacgact atcacattac actgaggaag agcaatacat
     8881 gaaactccaa tatcgtattc cattagctat atatagcgtc tggtgcatta tagcgatttt
     8941 gctctgcaag gatggcttgt accagatgaa catatggacg ctagtagaga tattggggat
     9001 gatagctctc ccgctgttag tccgtccgtt tttcattctg cttagaataa tatttaggaa
     9061 aaatagtttt aaaaccaaag acatgtctga caatcacaca gtatgtgttt tcctgagcgc
     9121 actcgcagat acttcctcaa agaaggcgat aaaaaaacac ttcaagcaat tactcgaaaa
     9181 gctgcccccc ttattacgcc agaagaaacg tatctacatg aaatcccatc ttctgactga
     9241 agccaggaca caaaagctga tttgctctct gcgccgaaaa gggctggatg taacagcgga
     9301 acgaagggaa tccgccatga atagcgtcat gttccgcatc ctcatcgtca gcagaatctt
     9361 atctcagtgg aaagtgcctc acataaatcc ccgctgtggt attgtcattc tgaccctgaa
     9421 aaacgattct ggaaacaggg aataacagtt ataagagcac cacgatttcc gacaggaaaa
     9481 catacatgaa aatcaacgta gatgaccctg gcaaaaagag cttcgctctc attacgcctc
     9541 acgtgcgtta attcccgaca caccccgtga cccgtcttcg cagcctgtac caccctttct
     9601 gccgttcccg ggattctttc tgtcactact cgcttgtgcc gttttttcgc aaccggtacg
     9661 tgcagttacg acggtacagc tgcttctttt cccgttccga aaggagtctt tgatgccaca
     9721 gcaacatccc ggacgactac aggtactggt cgtcgacacg cactgcaaac gcaagctgtt
     9781 ttcaacgaaa acacagacag atcccgatga gcttgcccga cgcttctgca cgccggataa
     9841 ctgccttgtg gtggtactat gcaacaaccg atttctgttc cgcctggaac gtgcacccgg
     9901 tagccactgc cgctggcgca agggtagccg ctcccgccat cagcacctgc aggactggct
     9961 gtcgtaaaaa caacaaaaac aaaagacctc gtcaaaacag gaggggacca tgtcgttttt
    10021 atttaaatta accatcgcga ttttgattgt ctttgcaatc ctttttccga ttttaatttc
    10081 atcagataac aaactgtccg cacttttttg gaagatacca aaagatttct gggttttcct
    10141 gattatcatg tacggggcaa tcatcacggt ggtgttcata gaaagcagtg aacaggcacg
    10201 catccgccgc caacaatatt tgcaacagta caaaatgcca aatgagatac atgaaaatgt
    10261 ggtgataaaa aacagtatta tttcaagaga aacaacagat gacgaatctg acattaacac
    10321 cctgtacctt gattcattta aaagtaaaaa caactgcaaa tcttttgaaa ccaattcaaa
    10381 tttactggat aaaaatggta agcctgtgga aataatggtg tgtgatgttc aggaactgca
    10441 ttttttcgtt ccaaagaatt aaacagttaa tcccccttac tctgttatct tagtcgccgg
    10501 catcaactaa catgaaaaat aataacgtac cattatatct atggttactt tggtgtacgt
    10561 tttgcctgta catcactacc ccgtggcaat acttccgtta tctatacact gaccacatat
    10621 cactatctta tatattatat ttctgtatag tggttattat ctgcttttcc ccactacttt
    10681 tcagatgcgt gcttcttatc gtaaggtctg tatctaaata caacttcaca ttctttgcga
    10741 aaaaaaatgg cagtaaagaa tcaagcatac atcttagccc ctatgccgat acacgttgcc
    10801 cggagtcaat gaggaaacac tgggatgtgt tactttctct gatatcaaaa gcaattaata
    10861 atgatgaaac cattgttatg cattcacatc tctttactcc tgcaagaata aataaactta
    10921 taaagagact ggaatcttat ggtttgaatt tctctgttat tacatgccca cggaaaacaa
    10981 gtctgtatga aagattatca attccttttg tatatctctt atttcagtgg tctctcccgc
    11041 atttgcattc ggacggaatg acagtcgtca ttcgtcctct ccagccgcct cacaccaatt
    11101 aacaaacacg tatatacgaa cacggagtct ccgttatgaa aaaaaactat ctgacactta
    11161 tactgccgtc attattgtcc ggctgtgccg gaatttccgg cagcaataac gtggcaccat
    11221 cagatacgcc tgctctggtt tttgttgacg gacaaatcag tgagagtgct ggcgttctgg
    11281 cacttacgca acaacgtctt tctccaccta aaataactcc ccccccgaga gtcagcgtgc
    11341 cagagacagt aaaaacagca catccagtca gcgaatcccg accgttacct tcaccaacaa
    11401 taagtcagaa tttctcccgg ttaatcatta caggcaagcc tggtagcgcc cctgtaacac
    11461 tatcgccgac ggcccgtaac cggaccgtca gtcagtggat aaaaacatta ctaccggaag
    11521 gatggaaatt cagacaagag aatgcagcca cgcccaagct gaataccaaa ctggtttcat
    11581 ggtctgccaa tgatcagtgg acccgttctc tgaatcggtt gctggaagaa caacacctat
    11641 ggggccatct ggactgggat aataaaacgt tgaccgtcac aacatcagct gctgcaccgg
    11701 cacaggacac ggcaaccact atctcgccag cagccatggt aaccacatcc acttcccctg
    11761 aatctccaac aacagcaaat agccagaaca aaccacgaaa tccattcaga ggaaacagcg
    11821 tatcttcgtc aacccccgcc gccaccccga ctccaaccgg ctccacagtc aagtctatcc
    11881 cgctcatgac cggaacgcct gttaaaccgg tatctcaggg taaagagtgg cgtgcgccgg
    11941 caggcaccac attaagagag aacattatca agtgggcaga agaaacaaaa tgcgaaagca
    12001 tggcatcaac gcactggatg gtcatatggc cactttctgt aaccgattac cgtctggatg
    12061 cgcctttaat gttccgggga tcatttgaat cagccatgga gcaggtattt gagttatatc
    12121 ggactgcgca aaaaccttta tacgcacagg cttctcgcat gcagtgcatc attacagttt
    12181 ctgatacaag ggatggtcgc taatgggatg gctggtcatg gccgtcggcc tgaccattct
    12241 gattatcact ggtagctacc agaatcagaa gatgtctgaa accacaaacg cccagcaata
    12301 tgcaagtgcg tctgtatggg caagtcagat actgatgatt gctaacagaa tcaatgatat
    12361 cagatacgta tcaggccagc aggacggagt aatttcctca gataaactgg ctctccctgt
    12421 cactccggac tcccgaatta aacatcagtt acaacagggg cgtctgtggg tatggatgcc
    12481 tgaacaacca ggtcttgttg aaactttgcg ttcaaaaagt cgtggttctg cactaatagg
    12541 gatcttccaa aacgggcaac tcacctggct atcaggaact gcaaccggtc tgacaccccc
    12601 ggcaggcatt actgctggtt ctgtcgtcta cgttaactaa tctggatata ttcatgaaaa
    12661 aatcacacca gcgttcaatg aagctggcgg tgctcccctg catgatcgcc gtcgctcttt
    12721 ccatctccgg atgtactttc agcgaaatca acaaaatgca gaaaaaagca caggaagact
    12781 cagcacatgc acgggaaaag gtatcagccc tttcggcccg taaatcgcag gctcttacct
    12841 ggctcgataa tcaatggata aaccctgttc cggtcgctca ggtatcaaga gagaaaaaac
    12901 aaacagctcc ggcctgctac atcacgcagg caagaaaagg agagatcact ctgcaggaac
    12961 tggggcaacg tattactgcc gtatgcggca tccctgtgat catcacgcct gacgcagcca
    13021 attcaactct tgaaggaggc gctacccgcc agatgacagg aacactacca gcaccagatg
    13081 aaaatgggcg tctaccgtta agcagcctgg gcagcacaac aatgactacc tccactcagc
    13141 cattaacgct gaataacctc atgtggcagg gagatatcaa tggtcttctg gatctgatgg
    13201 ccagccggag tggtctgtac tggcgcatgg ataatggtcg gattgtattc tatctgactg
    13261 aaaccagaac gtatccactt catatgctga acaccaaaac cagcagcagt tccagtgtca
    13321 gctctggctc aacaagcaca atgggggcaa caggaggcca ggataactca gcatccggtg
    13381 atgcaacgtc ctctcagagc acaaccgttg gtcaggaata cgatctgtat gaagacatcc
    13441 ggaaaactat tgaagcaatg ctgacaccag aaaaaggccg ttactggtta tctgcatcga
    13501 gctcaacgct gactgtcact gatactccag ctgtccagga agccgtcgca cgatatgtgg
    13561 acgaacaaaa cagtattatg aaccgccagg tagccctgaa cgtacaggtt ctgagcgtca
    13621 gcaataccag aaacgaacag ttcggtctgg actggaacct tgtttataaa tcgctacatt
    13681 ccgccggagc aacgttgaac aatgcaagcg gagattttac aggcgctaca tctgcaggcg
    13741 tatcaattct ggatacggca acagggaatg ccgccaaatt cagcggttcc agtcttctga
    13801 ttaaagcgct gagtgaacag ggcgatgtca gtgttgtgac ttcacaagaa agcactgtca
    13861 caaacctgac gccggtacct atccagatgg cagatcagac ggtttacgtc gcccagtcag
    13921 caacaacaac gactacggat gtaggagcaa caacaacatt aacgccgggc atgatcacca
    13981 ccggattcaa tatgaccctg ctgcctttaa ttcagaaaac gggcaatctc cagttgcaga
    14041 tgaattttaa tctgtcagat cccccaacaa tccgtagctt tacgtcaaaa gacggaaaca
    14101 gttacatcga aatgccgtat accaaactgc gttcactgag ccagaaggtc aatctgaaag
    14161 aagggcaatc acttgtcgtt actggtttcg atcagaacaa tacgacgaca agtaaagccg
    14221 gtacgtttac gccagcaaat ccattatttg gtggttcaca aaccgggaaa aatgaacgca
    14281 gcacgcttgt aatcatcatt accccgactt tcccgtcagg aggcaacaat ggctgatgaa
    14341 gatatcaacc cggttgtact tctcgcagat cctaaagtaa atcatcgcgt gtgggcagcc
    14401 tgtcttaaat ggagtcctgt cgtaaaaaaa cagcgagtcc cttctcatca gaaacacaag
    14461 ccccatgtaa aatccagacg gcttacctct ctgaaagtga ctgtaggcag cagaagttcc
    14521 cgtgggaaga tcagccgcat taccggcaca ggaatactgg ccaggccgga gcgcaatcat
    14581 tatttttccc ttgctctggc tttctgctca tgggtgagaa atggatacgg cgtcttccgg
    14641 tacagcgata aggagctttt attccttgcc tccattaatg gccagcctgc cgttatggct
    14701 gatctgtcag gaaatgatgc tgatgttgct caaaaggtca gtctttttct gacgatgaac
    14761 gaagagcccc ccgaaaaatg gcaggtcgta tctcctctgg aacatcctga taactgggaa
    14821 agtattatta cccggttatc atcagcagat ctccgccgtt gcaaacttac tgtcggcaat
    14881 cgcagcaaat tcacactgcc tgccgtactt tttttagtag cagccagcgc aggcactgta
    14941 ttctggatga ctcagccaga gccggatgtc gtaccgacgc ccgaggagat agctgcacgg
    15001 gcaagattgc agtttaaaaa gcccgatcca ccgccagaac tcccgcatcc atgggcatca
    15061 caacctgtaa tcagtgattt tctgaaagcc tgcgcggacc ttcgaaaacc atcaccggtc
    15121 gcgctagaag gctggaaact taccggtgga acatgtactc ccgagacatt cacactgatt
    15181 tacgaacgac agccaggagg aactattgaa ggctttctgg caagaagcaa agaaatcttt
    15241 aatgttattc ctgactttaa ccttaaagat ggtgcacgcc tggcttctgt aacccggcca
    15301 ctgccttccc tgccccgcag ggatgaggca gtccctaccc cctcagaaca actgatgcgg
    15361 gtgttcacgt ggtttcagaa aaaacagtta acgccggcca ttaatgaaat agccataccg
    15421 gaaccattac caggtaacga tggtgaacca gcccctgttc agaaatggaa agaatatcag
    15481 ttcagtttat caacgcctgt aaatcctgat gagttgttcc ctttatttca ggataccggc
    15541 gttcgtctga gcaatatcca ttttgaatta aacggcggta ctttcagtta ttcctcagaa
    15601 ggtcatattt atgcgtccag gtaaattact cattattcca tccatatttt tcttctccgg
    15661 tttttcattt gcaacaacac aacctcttgt cacaatagga gaacttgaag cacaacaaaa
    15721 ccggaatatt cttttacagg caaaagttca gggcgcacag ctacagaaac aactcgaaga
    15781 atcagatgtt acttcatcat ctgaaactgt ttcaggtttc tctggtgtga ctgcctcttt
    15841 gccatcagtt tcagagcagc cgacatctaa aaacagaaag gaattgcccg ttattatgga
    15901 aataaacggc aaagataaac gtcttaatgc cgttcttcgc atggctgatg gacgtcagac
    15961 aagcgtcacc actggcagcc agcttccagg cacatccgtc acagtgaagt cgatctcatt
    16021 gtctggcgtc acgttaagtg atggcaccac ccttactttc tgagtaacgt gatatggata
    16081 aatataatct gaaggatgct ctccttttta tatccagggg agacacccat gaaattctta
    16141 tagaaacaaa ccagcggaca aggcctgacg tacaaagtaa tctccaggag ctgctaaaat
    16201 tatatccgga tatcaaccca aaagtagtct cgttgtcgga gttacaggag gcaggtcagg
    16261 atgagaaaaa cagaggcccg aaagaacgaa ttattcatct gaaagacctt gctgatgtca
    16321 gtgaaagtga gaagaaagtt ctatcttatt ttgaaactgc cagaaaactt ggtgcttcag
    16381 atattcattt cttaatttca gagtctattt tcaaagttcg catgagaata ttcggtgaac
    16441 tgcagactgt agatgaagat caaccagcac tgggctattc actttgcgca acagccattc
    16501 tgtcaatggc tgatgtcact gaaaccagtt tcttccctca aagggagcag gatgcgcgcc
    16561 tgtccccaca attaatgcga aaaataggaa tatttggtgc acgctacagt cacagaccta
    16621 cgggagacgg tctgattgca gttatgcgcc tgatacccga tgacggagat aaagttccca
    16681 cgtttaaaca actgggcttt atcccggaac aaatccggtt gctgaacata atgctgcgcc
    16741 gcccggaagg aaaaatagtc ctgtcaggtc caaccggttc cggtaaatcg acaacgttac
    16801 gcagcgcctg ccgtgtatat cttgacgata atcaggggcg acacctgcta acaatcgaag
    16861 atccgctgga aggacagata ctgggagcaa cacagactcc gattatctgc gacaaatccg
    16921 acgaagatgc cgtcaaactg gcatggagtc gggctatttc atcggcaatg cgacttgacc
    16981 ctgatgccat catggaaggg gaaatgcgcg atcttatctc gatgatgtca accacctacg
    17041 cggcacagac cgggcacata gttctgacga cattacatac aaactcagct ctgggtatac
    17101 cggagcgaat gattacgatg ggtatgaatg ccgatcttat ttgcgatgcg caattactga
    17161 ttgggatgat cagccagcgc cttgtaccaa ctctgtgtcc ctcctgccgt attccatggg
    17221 aaaagcgagc acctgaactt agcgacgacg agcgtgatta ccttgagcgg cactgcaata
    17281 aagattcact ctgcagtaca gataatatct ggttccgaaa tcatcacgga tgctctgaat
    17341 gtaatcacga cgtaatcatt aacggcagaa agcgcggtga aataggtaaa ggcctcaccg
    17401 gcaggactgt aattgctgag gtgatagaac ctgataaccg cttatttcag attctgaaaa
    17461 cacgcggcaa agttgcagca cgtaaatact ggctggaaaa catgaaaggc atcagtcgtg
    17521 tagagcatct tcttcgccgt atcaatgaag gtctggtaga ccctcttgaa gccgacagaa
    17581 tcatccctct ggacgaagac gagcgcctca gcatcgatga tgtctaacag gagaacaaca
    17641 tgcgtgaaat gaatttcagt caacggctga ggcgtttcat tgtcagaaaa acgttcagcg
    17701 ccccctacag ggtgcagttt tacgaagccc tgcgttttct gctggaaaat aaacagccat
    17761 taaagaccgc actggaacag atgcgggatg cctggacaga ctttggacga aaatggcatc
    17821 cctttgctga actggccaca gactgcattg agtctcttcg tgaaaacagt ggcgaaaact
    17881 ctctggaata tactcttagt ttatgggttc ctcaggaaga ggccgcagta atcagcgccg
    17941 ggataagaag tggttcaatt gtggatgcac tgcaattcgc caccactctt acagatgcaa
    18001 aagaacaaat ccatcaggct atatggcaaa tggctattta tccggtggga ttgttaatca
    18061 tgatgacggg gacattatat gttctgaata cagagctaat acctgaactg agcaaaataa
    18121 gctcccccga ttcatggagt ggtgctcttg gttttcttta tggattatct gtttttgtcg
    18181 ataattacgg cgctatatgt gccgttcttt ttgcagtgat cacaggttta atatcatggt
    18241 ctctgccaaa ctggaaaagc cctgacagtg ttcgtacttt tgccgacaag ataatgccat
    18301 ggagtattta tcaggatata cagggagcaa catttctcct gaatatggct gcacttctga
    18361 aagccaaaat gacgacgctc aactcgctaa acattcttca ggagtttgca tcaccatggt
    18421 taagcacacg tcttgacagc attatttacc gcgtccgcca gggggatcat cttggactgg
    18481 ctttgcgtca gtgcggttat cagttcccat ccagggaagc agccaacttc ctgtctttat
    18541 tacagggaga tggcgcaaca gagctaatca gcaattatgg tcaacgatgg cttagtcaaa
    18601 cacttcaacg tgtaaagaaa cgggcaaacg tgattcgttt aattatgctg atatttcttg
    18661 tcatgtctct catgttactg gtttttgcca ttatggatat tcagtctatt agtgacaaca
    18721 gcatgggtaa cttttaaaca aaacgaggat atttatgtta gtagaaaata ttaataccac
    18781 tttaactgga aataataaga agaatgagcc tcatgataaa ggatgggcaa ttttggagca
    18841 gggaacaata gcgctcgttg ttctttttgt tatagtcgtt gttctgggta gtttgtatgc
    18901 gttgcgtaca agagccaatg tggcaactga aaccgcaaat atacaaacaa ttatcacaag
    18961 tgcccagagc ctgctaaaag gtagcgatgg atatacattt accagcagtg ccaaaatgac
    19021 aggtgccctc attcagatgg gtgtcattcc ctctgggatg accgtacagg gcgacaaaac
    19081 atctggtaca gcaacgctct ataacgcatg gggcggtgct gtaactgttg ccccggcttc
    19141 aacatcaggg ttcaacaacg gttttaccgt tacctatgat aaagtcccac aggatgcctg
    19201 tatccagatt gccacacgaa tcagtaaaac tggtttaact aacggcatta ccctgaacag
    19261 tactgctcac agtgatggaa aagtcactac tgaagaagcc agtacgcaat gcaaggcaga
    19321 taatggcagc actggtacaa acaaacttat cttcaccatc aatggttaag gagttttaat
    19381 gtcagggggt acagtacccc tcagacttat ttcattaatc ctgctttttt ctcttattcc
    19441 acccgcccga gcttcatacg tagctcccaa acagatctgg accaacaagt gggacaattg
    19501 ctttgccgct gccggcgcgc gctatcaaat tgaaccatta ttattgaagg ctatttctgc
    19561 cggggaatct tcgttgaggc cgggcgcaat taatataaat aaggacaaaa aaacaggtaa
    19621 agccagtagc acggattatg ggttgatgca aataaactca acccatattc caaagctgat
    19681 aaatatgggc gtaataaaaa agtctgagga tttaattacc aaaccatgcc ttaatatcca
    19741 tataggaagc tggatactgg caagacattt tcagatatgt ggcgtcagct ggaattgcct
    19801 tgggtcatac aatgccggat tccgtaagga cagacatgaa acaagagagc aatatgccaa
    19861 taaaatatgg agaatttatc gtgacatgaa agggatatgt cttcccggac agggaggcag
    19921 gcaatgcaga caatcgtagg cataagtcta tcccctgttt atatactgcc attgatgttc
    19981 acattttcca ttattggacg aactctgcta ttcagagttc gttatttttt atctgatacg
    20041 ggacatctct ggtataagac acaccctgct gttctgtcag gaatatggct atacagtata
    20101 gctgtggcgt taattatttt atcatcatca ccactgctct accgtattca tgctgtcctt
    20161 attctttcgt tcatactgca aatggcggta acggatgcac tgacaggttt attaccagga
    20221 acattcaccc gacgtttttt aattgcagga atgctatcac aaatcacgac tgatatatgg
    20281 tggttccgta caacagaatt tgccactgct gcaattgtct tattctgcct tcataaatta
    20341 gttaaccgcc atcgtcttaa tattggcact ggagacctgt ggcttattgc aggtattacc
    20401 gcatggtcag ggttatataa cgccatatgg tgtgtgttgc tgggcacagg aggatttgtc
    20461 ttatggcatt caacatggtg cataaaaggg cataaagaag gccctctggg tccatggcta
    20521 tgttttggcc atgtcctttt gctgctggat aatctttatc aaccactatg ggtaatctga
    20581 tgaaaaaata tgaccgaggc tgggcatcgc tggaaactgg cgcggcttta ctgattgtta
    20641 tgctcctgat tgcctgggga gcgggcatat ggcaggacta tattcaaaca aaaggatggc
    20701 aaactgaagc acgtcttgtc agcaactgga ccagtgcggc ccgctcttat atagggaaaa
    20761 actacacgac tctccagggc agtagtacca caacaactcc tgccgttatc acgacaacca
    20821 tgctgaaaaa taccggcttt ttgtccagcg ggtttactga gacaaacagc gaggggcagc
    20881 ggttacaggc atatgtggtt cgaaacgccc aaaacccgga attactacag gcaatggttg
    20941 tatccagtgg tggcacacct tatccagtga aagcacttat ccagatggct aaggatatta
    21001 ccactggtct tggtggatat atccaggacg gcaaaacagc cacaggtgca ttacgttcct
    21061 ggtcagtagc tttaagtaat tatggtgcca aaagcggtaa cgggcatatt gccgtattgt
    21121 tatcgacaga tgaacttagt ggtgcagctg aggacactga tcgtctttac agattccagg
    21181 tcaatggtcg ccctgactta aacaaaatgc acacggccat tgatatggga tcaaataacc
    21241 tgaataacgt tggggcagta aatgcccaga caggtaattt cagcggcaat gtgaatggtg
    21301 taaatggcac tttcagcggt caggtaaaag gcaatagcgg aaactttgac gtaaatgtca
    21361 ccgctggcgg tgatatcaga agtaataatg gttggttaat tactcgtaac agtaaaggct
    21421 ggctcaatga aactcacggt ggcggatttt atatgtccga tggatcatgg gttcgaagtg
    21481 taaacaacaa gggcatctat accggcggtc aggtgaaagg cggtactgtt cgggctgatg
    21541 gtcgccttta tactggtgaa tacttacaac tggaaagaac tgccgttgct ggcgcatcat
    21601 gttcgcctaa cggccttgta ggccgcgata atacaggggc aat
//
LOCUS       DAZGCS010000081        21057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGCS010000081 DAZGCS010000000
VERSION     DAZGCS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.081"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..514)
                     /locus_tag="ACHK01_003335"
     CDS             complement(92..514)
                     /locus_tag="ACHK01_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8098243.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(561..986)
                     /locus_tag="ACHK01_003336"
     CDS             complement(561..986)
                     /locus_tag="ACHK01_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8098244.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGQDFLYAEASVYALAKQMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(1404..2180)
                     /locus_tag="ACHK01_003337"
     CDS             complement(1404..2180)
                     /locus_tag="ACHK01_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098245.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLAWDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(2226..2660)
                     /locus_tag="ACHK01_003338"
     CDS             complement(2226..2660)
                     /locus_tag="ACHK01_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8098246.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            complement(2674..2895)
                     /locus_tag="ACHK01_003339"
     CDS             complement(2674..2895)
                     /locus_tag="ACHK01_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098247.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(2896..3579)
                     /locus_tag="ACHK01_003340"
     CDS             complement(2896..3579)
                     /locus_tag="ACHK01_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO8098248.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3655..3891)
                     /locus_tag="ACHK01_003341"
     CDS             complement(3655..3891)
                     /locus_tag="ACHK01_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098249.1"
                     /translation="MRRLTRRGINAACGQCPASGRARDGRFYRSRLASVTVYASLSPF
                     SDERPSSRFRGITLPSERRRLRYSTVGLTRYRTR"
     gene            3783..4013
                     /locus_tag="ACHK01_003342"
     CDS             3783..4013
                     /locus_tag="ACHK01_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFO8098250.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLPEKKRAVARPSPSYFIRHNAPPCRLSTQVR"
     gene            complement(3964..4866)
                     /locus_tag="ACHK01_003343"
     CDS             complement(3964..4866)
                     /locus_tag="ACHK01_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO8098251.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            5284..5463
                     /locus_tag="ACHK01_003344"
     CDS             5284..5463
                     /locus_tag="ACHK01_003344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098252.1"
                     /translation="MIRIEILFDRQSTKKLKSGTLQALQNEIEQRLKPHYPEIWLHMW
                     ESPSFRVRSCQPALH"
     gene            complement(5547..5939)
                     /locus_tag="ACHK01_003345"
     CDS             complement(5547..5939)
                     /locus_tag="ACHK01_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO8098253.1"
                     /translation="MYEGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(5943..6917)
                     /gene="parM"
                     /locus_tag="ACHK01_003346"
     CDS             complement(5943..6917)
                     /gene="parM"
                     /locus_tag="ACHK01_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO8098254.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRNNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            complement(7156..7530)
                     /locus_tag="ACHK01_003347"
     CDS             complement(7156..7530)
                     /locus_tag="ACHK01_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098255.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            complement(7530..8162)
                     /locus_tag="ACHK01_003348"
     CDS             complement(7530..8162)
                     /locus_tag="ACHK01_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8098256.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            complement(8746..9531)
                     /locus_tag="ACHK01_003349"
     CDS             complement(8746..9531)
                     /locus_tag="ACHK01_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8098257.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
     gene            complement(9533..9946)
                     /locus_tag="ACHK01_003350"
     CDS             complement(9533..9946)
                     /locus_tag="ACHK01_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098258.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            10515..10775
                     /locus_tag="ACHK01_003351"
     CDS             10515..10775
                     /locus_tag="ACHK01_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098259.1"
                     /translation="MDEERVFSLSYEQLTRFTERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDWK"
     gene            10772..11362
                     /locus_tag="ACHK01_003352"
     CDS             10772..11362
                     /locus_tag="ACHK01_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098260.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLTEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLEVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
     gene            complement(11380..11727)
                     /locus_tag="ACHK01_003353"
     CDS             complement(11380..11727)
                     /locus_tag="ACHK01_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO8098261.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLCILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYRGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWGDV"
     gene            11846..12193
                     /locus_tag="ACHK01_003354"
     CDS             11846..12193
                     /locus_tag="ACHK01_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO8098262.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDSQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            complement(12211..13485)
                     /locus_tag="ACHK01_003355"
     CDS             complement(12211..13485)
                     /locus_tag="ACHK01_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO8098263.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLKTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNVSARTNEQKHANDALNALLK
                     EKENIRSQLADINQKIAEEKRKRDEINMIKDAIKLTSDFYRTIYDEFGKQASELAKEL
                     ASVSQGKQIKSVDDALNAFDKFRNNLNKKYSIQDRMAISKALEAINQVHMAENFKLFS
                     KAFGFTGKVIDRYDVAVELQKAVKTDNWRPFFVKLESLAAGRAASAVTAWTFSVMLGT
                     PVGILGFAIIMAAVSAFVNDKFIEQVNKLIGI"
     gene            complement(14370..15716)
                     /gene="yagA"
                     /locus_tag="ACHK01_003356"
     CDS             complement(14370..15716)
                     /gene="yagA"
                     /locus_tag="ACHK01_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO8098264.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRLRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            16070..16672
                     /locus_tag="ACHK01_003357"
     CDS             16070..16672
                     /locus_tag="ACHK01_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO8098265.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            16728..17222
                     /locus_tag="ACHK01_003358"
     CDS             16728..17222
                     /locus_tag="ACHK01_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098266.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMELAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            17222..17488
                     /locus_tag="ACHK01_003359"
     CDS             17222..17488
                     /locus_tag="ACHK01_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098267.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(17965..18456)
                     /locus_tag="ACHK01_003360"
     CDS             complement(17965..18456)
                     /locus_tag="ACHK01_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098268.1"
                     /translation="MAQQRQDRRGEVQAATASATGLACFIFRFLRRPLAEVANYSNDR
                     WEAPQRASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQW
                     LVVEVFGEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKK
                     SKK"
     gene            complement(18390..>21057)
                     /locus_tag="ACHK01_003361"
     CDS             complement(18390..>21057)
                     /locus_tag="ACHK01_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFO8098269.1"
                     /translation="REHLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASA
                     LIGHLRRQSVILPALNAVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDN
                     GKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQ
                     MTPADLAKFEPQRRYATLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQA
                     SGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDF
                     DFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAP
                     TGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPP
                     EKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLK
                     ITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKN
                     LLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHP
                     FAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVV
                     NVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDT
                     KLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSY
                     PRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLG
                     EIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLG
                     WEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         5072 a         5624 c         5295 g         5066 t
ORIGIN      
        1 gtggtgagcg gtttctgccc catttcacga cgccgcgcat taatcacggc tttgtaattg
       61 cgcagaattt cgcgggtttc tgtgctgata ttcatgacat gtgctccccg tccagcgcct
      121 gtaacacaac gtccattgct tcgcgttctt ccctgacgaa cgcatcaccg tcgcccccgt
      181 tctcactccc gacggcatac aggcgcttca tttcactgcc tgccagcgcc aacacttttt
      241 ccagcatcac cgccgggact gtcacctgac ggctggcgcg cttttcttcc atcagttcac
      301 gaacaacgcc gtgaacaacg ctgacatccg caccatattc aaaggcatcg tccagccgct
      361 gcatgacggt ttccagctca tcgtctgtca gactccagtc ctgggctatg tggcgcacat
      421 ccccacgggt cagcaccacg gcaaaaacgc attcactgtc cggcacgtct gtatccagta
      481 catcgcggat aatttcttta acagtacagt acatgttctc gtctccttta caggggcggc
      541 attgccgccc cgtccgttaa tcagtccagt gctgcgtaaa tcgcgttgca ttcagggtga
      601 aattcgatgt gacgccacaa ctgcgcatcg cgcatccggt aaagctgggt cagtcctgca
      661 tccccgctgt cgtggtacag ccacaactgg cggttaatca caagggaggt aaggatgatg
      721 cctgcggcat cagcactcac ggtacggtca aaccagtttg caccgttcac catgtgaaaa
      781 cggtcgccat ccggcatcat gtaaccgcca ccgtccggca gtcggatgaa gtgccagaat
      841 ccgccttcat attccggcat catttgtttc gccagcgcgt atacagacgc ttcggcatac
      901 agaaaatcct ggccaaacag gtaaggcaga aaagacagac ggtcgcactc ttcaacgttc
      961 agagtgacag gtttcgcata ttgcatgatg ttttcctccg ttgttcacat attccgcgac
     1021 accgggcggt gccgtgcctc cataaaagcc gggcgataag gtggggatgc acagggcgca
     1081 agggaagccg cagcctgacg cacacggctg gcgggagttg gagggtgaga ccgcgaaatc
     1141 tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag
     1201 cggcgaagcg tacccttgca tccctgccag cccggctaag atggaggcac ggcacagccc
     1261 gcacacgaca cggcggccac ataactgaaa aaccacaagg ccatgccctg catggccggg
     1321 taaagcggag ccggcggcga cgtcctttgt gctctgtccg gggaccggca ggaaaaaggc
     1381 ggggatttac cccgccagtg tcatcatttc cggatttttg ccagggcatc cgccatgctg
     1441 cggatttcgt tccgggcttc ccggaccacc tcccgcagaa caccacggtc aaaccagcgg
     1501 cggggcggtt catccggtat acgcagcaca tcccaggcca gccaactgga caggatatgt
     1561 tcaatacaat cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg
     1621 atggcgtcga gcaccacggg caggttttcg tcgtcctcat cgagccagca ggggtgatcg
     1681 ctctccttca cgaccaggcg aaagcggcgc gtctggcggg tcagtaacgt ctgaccggtg
     1741 gtggtcagcg ccgcccggat acgccgcgta tcttctgcca cctggtaact gacacagcgt
     1801 actgcatcgc gtaccgcatc aagaactgcc ctgatttcgt cctctgccag ccactcaccg
     1861 gcatacggga aagacacctg cgttccgtct gccggaagta ccggcagcga ctgccactga
     1921 ctgccggaca gtacaagttc cgccccggtt ccggtcagtt tgtgaatggc ggaggccagg
     1981 agcggcggca gtgcgccgcc gtgacggttg ataagtaacg tcgccagccg gggcgcatcc
     2041 agcagaacat aaatgctgat ccagcccgga tggttcgggt tccgctggtc aacatccgtc
     2101 tcttccggcc agccctgcgc cagcagcagg ttgcgggcgt gacgacaaat cagtgcgttc
     2161 agtgttgtgt tttcgttcat gattaatcgt tctccctgaa tcgccgcccg gaggcggcga
     2221 taacatcagt cgttttcgtg tctgttgttt ttcagcaggg tgcgggcctg attaatcacc
     2281 gcctgacggc gggtgacgca ttccgggacg gcgaggccat gatcccgggc ggcccattca
     2341 cgtttccaca atgcctgttc agcggtctga atcagggacg ccagcagttc agcagggacg
     2401 gtcacctggc ggttttcgct cacgttattg atgatctcca tcgccacgcc ggaagaaata
     2461 ccggattcag tccgctggtc ttccgggatg tcctccaggc gcgccaggac ggttctgatt
     2521 tcatgatcgg acagggaaat atccattccg tcagcgaggg cctgaatctc ttccggggac
     2581 cagataacca gcatcagcgg catgtctccc ggatacttta ctgccagctc ccggcatagc
     2641 gtttcgcatg ttccgtacat tctcgtctcc ttctcaatct ttaaagcacg cttccagttc
     2701 cagaatttcg gtatatgcct ccatcaggag gcggtttgcg cgaaacagcg tctgacgcac
     2761 cagacgttcc ggcagggtgt cggcattaaa gctgtaccgc tgctccatct cattcatggt
     2821 tgtacgcagg tcgcacatgg cattggccac ctccgccact tcggcgcgca gtttttcgtg
     2881 tcctgcatag ttcatttacg cagcctccgg cataaaccag tcatcattcg cggccccctg
     2941 ctgcatggcc cgttgcacgg cggcaaggcg ttgctgcccg gcacggtgat actgctcaag
     3001 cagctcgata ccgatatacc ggcgtccgga ctggagggcg gcgacgcagg ttgagccgct
     3061 gcctgcaaac gggtccagca caattgcgtt cgggtgtgtg aagttctcaa tcagcggttg
     3121 caggctggta acgggctttt ccgtcgggtg atggcgattg cccgaatatt tccagcccag
     3181 cacgtccggc agcgggtttt gtggcagacg tggacggcct tttgccagga tgtaggcgca
     3241 ttcgtggcga tagcccacat atgcggcctt cgatgtgtag tttttggtga agaccaggtg
     3301 accaacaacg ctgaatcccg catttttcca ggcggccata aagcgatcga cgcggttcca
     3361 gccgtagaag ctcaccatta acgcgtcttt tttcagtacg cggaacattt cattacaggc
     3421 cggttgcagc cactcatcgg ttttatcgcc ggcgatggtg cgcccggaac ggtcacggaa
     3481 accgacgaga tatggcgggt cggtgaggat gaaatcaatg gcgttatcag ggatacgggc
     3541 cataacatcg atgcagttac cgaggacaaa acgggacata ccaaactcct tctcttacag
     3601 agggcaccgg gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg ctgttcaccg
     3661 cgtccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt ctgacggcaa
     3721 ggttatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgaca ggctggcgta
     3781 cactgtcacc gacgccagcc gggagcggta aaagcgcccg tcccgtgccc ggccggacgc
     3841 gggacactga ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag
     3901 gagcacggcg acatccttta ccggaaaaga aacgggcggt agcccgcccg tctccgtcat
     3961 acttcatccg ccataatgcg ccaccttgtc gactatccac gcaggtccgg tgacttccgg
     4021 cagctcatcg tcatctgtcg gggaagacca ttccagctcc ccccacagca catcaacgag
     4081 ctctccgcgc tcatacaact gccagccaca gaaatcacag ccctgttcgg cataccagtg
     4141 ctccagcgtg cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt ccggctgaca
     4201 ccacggcgtg tcgaaatcaa cctgaatgaa gttgtctccc tgactgctga tattcaggtc
     4261 atacccacaa ggccacttca cgccatactg ttcggtatac cagtgatatg cagaaggaac
     4321 accgttcaga acaccaccgt taaagccatt cacttcaaca tcaaggcggg tgggcagaac
     4381 cgtcagcagg tcaaacggca tggttttttc cggcagtacg ttgtcacaca gacggttcca
     4441 ccacaccgat acatcctcgt tactccagaa gccacaccag tcacctcttt ttgcggtgaa
     4501 aagcgcggtg atggtatccc tgacatcgtc cggtaatcct tcccagcgac gctggccagt
     4561 accgctctgc agccagagtt catgcagcat caggcagttt tgctcatcca gcagtacacc
     4621 gttctgcagg tgtgtcagcc agcgggtgaa cgcgatattc tccggcgata caacgccacg
     4681 tccggcagcc gtcagtccgg ggcacggttc aaaccacaca tcttcagtgg tctgcagaag
     4741 tccggcactt ccggccagaa acagctgaat accttcattt gtggcgcggc gataaaacgg
     4801 tgtgacggca ccactggcca gtcgtttaat ctcagcaatc tgtgccggtt caccagaaaa
     4861 atgcatacgg ttactgcacc agtttggcat gggaattctc ctctcacatg cggcctccat
     4921 caggccgtgc cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc
     4981 cgcagcgcgt ggaggaggaa cgacgaaacg tgaccgggtg aggaggccgg cgacagcgac
     5041 acacttgcat cggatgcagc ccggttaacg tgccggcacg gcctgggtaa ccaggtattt
     5101 tgtccacaga accgtgcaca aaatgctgtg gataagctgg acacagcact ccacagcagg
     5161 catacagcca taccctcaga gaattgagcg ttttttagtc agaacaatga catttgactg
     5221 tgtgcggatt cagttattag aatactgtat atatatacag caaaagaaaa ggaagatgag
     5281 aacatgatcc gcattgaaat tcttttcgac cgccagagca caaaaaaact caaatcaggc
     5341 acacttcagg cgctacagaa tgaaatcgaa caacgtctga aaccacacta cccggaaatc
     5401 tggctgcata tgtgggaatc cccgtccttc agagtgagga gctgtcaacc agcactccat
     5461 tgactcaaat gacgggaaac gcggaaatct aaaaattaag ccgcacaccc cgagaaattt
     5521 tgcaggtttc acggagcgag acttttttag atcaggtttt gcatcttcac cctagccgct
     5581 ttcagacggg cctgttcacc gtcatcctga gagtgttctg gtgaaactgt actctgaagc
     5641 cctcccaacg ccttcagtac ctcttttaaa tcaccggagg agggtttcca gcctgttatc
     5701 ctggcaatct gactaatgac ctcatccgca ttcaattctc tggtgaatag cgcggttaac
     5761 acggcgggaa ttcgctcatc aagctgatgc attatcaggc cggttatcaa agcctgccga
     5821 tataactccc cccgtttttt ccggggaaca gtttcgattt cagccagtgc aagacaatcg
     5881 gcagagtcct ccgggtgaag gtagagtgaa agttttttcc tttcttcgcc ttcgtacatc
     5941 gattactcct ccgctttgaa gcgtgcaata gcctggacca gcgctgtctg aggttcatcc
     6001 ataagaacca ccttttcgcc aaggtggttc catgccttac gaacagcatc agcaatgagt
     6061 gcagccccgc caccgacaat atatacgcgg tttacgtttc gatactgcag caaatcatca
     6121 acaactagct cgcccagttt atttatcgct gtatcaatgg tatccagcac cagatttagt
     6181 ttagagacct catttacaac ctgccccaca aaatccaggt tattcctgtt tttgatcaac
     6241 tcgtcagcaa tcattggact ggtatcactg ctggccattt tcagtgcagt gagcgtggct
     6301 ttcgtgacca ttgatacacc aatatcagga ttaccatgta cagccgaaac atcctcaaac
     6361 tggccaacaa taaccccgac atccagagtt gtaccaccga gatcgataac caatgatttt
     6421 tcgtactgac caacgttgtc agcaactagg cgagtaaata cagccgggag tgattcaggc
     6481 atcacttcaa cgcttttaat ggtaaaagtt ccccctttgt tcagggtgac ttctcgcatc
     6541 aggttgcgaa tttttctctc aatgttaagc gtatttttct ggcagtcggc cgtgtaaaac
     6601 tcgctgatag gtaacgtaac ggtgagatcg atctcctggg gttcaatgcc actgttcagt
     6661 aatgcgtggt gaactgccaa cacatttgta tcagagtact gatactcaat atgagtcgtg
     6721 ctgatcgcat cttcgctgac cgggtcgtag gtatatttac ggccatcaag cagatagtta
     6781 taagtacgac tgctgcctaa gccctcgact ttccagttat ggcgaaaaga gttcactgat
     6841 accgcacttt tcagcgtctt gccttcaaac caggcaagtt tcacatttgt agaaccatca
     6901 tcgcaaaaaa ttctcatacc atctccattt atactcaact tgagtattca tctttacaca
     6961 ataactactc acattaagta aatacgctca taaaccacag gtaagtactc aagctgcgta
     7021 cttcacttgg gcagttaact actcaatttg agcattatat catttatctt gcagcaatct
     7081 agcatttatc tagcataaat cttgctgcaa gattgcacac aaaaaaagaa tgcagcaaga
     7141 ttgctgcatc ccggattaat agccctgttt ttccttatat gcctcaactg ctttttccag
     7201 aacttccacc agtttcaacc cctcacgctc tgcgatatcc cggatctctt ggtcgaaagc
     7261 aggtgtgaca cgtgttgcaa atgccagcgt cctgtttgtt tttctggcag aacggccatc
     7321 gcggcgggta tatacctcgg tcactgtttg cgtaaccaca ggctcatggt gcactggcga
     7381 tgagtctgcc tgttgtctct gaacatcagt ggcagcgaca gccggagcta tctcaggcgc
     7441 attcaagctg gtagccactt cgtcggggcc cgggggtacc cccaacccac tacgggttct
     7501 tttagttcta agttttgacg catcagccat tactcttctc caatcttatc gatgagtgcc
     7561 tggataagtt catcagctct ctcgtttaaa cctttataac gtgtttctgt cacagcgagt
     7621 cctgaattca tcgcttttct gtatgctggc ttctcaaaaa ggcatccaga taaagtttca
     7681 taacctgctt cactgatata tgcacgagca tccagctctt ccgcttcggt acctacccgg
     7741 cttaaagcga agaccagttt tgaacgtggt ataccttctt ttaccaacgc gttgaaaact
     7801 ttaattgccg ggacaagatc gtcaagggag gctcccgttg gctggacgat aaggtcggcg
     7861 accttagcaa tttctgaagt acctttactt gcacgagcag ggccatcaag aatcagaagg
     7921 tcaaaatcgc cggcagattc tatcgcctgc tttgctgttg aaaacacttc aacggaagca
     7981 accggtgaca aaccagctga cagacgtcta cgatgccact cagcactcgt tgcctgttcc
     8041 aagtcgagat cagctaattt tgttttgata ccacaggcgg atgcttcacg cgctaacgct
     8101 ctcgctttag tgcttttgcc cacaccacct ttctgagaaa taaaaccaac tatccaggcc
     8161 atttagcccc ctttacttgc tgctatcttg cagcaaacat caatataaac cagattgaga
     8221 ttaggtagag taactcttcc agaacaagat aacagcttgc agcaagattg caacaataaa
     8281 taatcttgct acttagcaga gtgctggaat tgacagccga atacttgtgt cacgttaaca
     8341 ataaaagaaa cctcattcag aatcgcatcg acagatgaag caagattgcc gcaagattgc
     8401 agatagcata acataaacag agcgctaggc cgtatgtgta cagcatgata gctgcaatct
     8461 attttattgc tgctatcatg ctgaaatatc attattagaa tcaggcagcc ctcgccgcag
     8521 ccatgtaact gtgcgtagcg agcgaactgg cgaggaagcg ttccggcaga tggctcttac
     8581 gctcggcaca agaagagttt cccatccagg caagtactat gggttgtcag cgccagagat
     8641 ataagactgt tctggcctga gaacgtggcc tctatgagtt tggtgctcta tcttccatag
     8701 atgaatgcac cagctccccc ttgactgaac cgcgaatacg gctttttaac gcctctcaag
     8761 ttgttttatc agcgctaccg catcggcttc cggcatggcg aactgcacac gatgacgcgc
     8821 cgcgacatcc agcgcaaaga ccttcgtgta gacctccgtt gagctgatgg acttatgccc
     8881 catcagactc tgcaggacct tcagcggtat accggcatac agcatgtgca tcgcatagga
     8941 atggcggaac gtatgtggcg tgaccgggac agagaacgtc actccatcag cggcagcggc
     9001 ggcaaccgcc tccccaatcc aggtcctgac cgttctgtcc gtcacttccc agatccgcgc
     9061 tttctctgtc ctgcctgttc gtttattacg ccgttccatg gggattttca gtgtggccac
     9121 catcgtctgc agctggctga cgtaccaggc gtcagagagc ggaaccagcc ggtgagtctg
     9181 ctgaccggcg ggcattcttc ctgccgtcct ggcggctttt tcggtccgct gcttcagagt
     9241 ggccaactgc acaaacggat acggaggcgc aagcgaaaaa tccccccgcg tcagcgccag
     9301 tgcttcatta atgcgcgcac cggtgttcca cagtgtggcc agcagcattt tgcggcgcag
     9361 atccggcacg taatggagca gggcgctcac ttcaggggcc agcaggtatt ttggcagttc
     9421 atcatgaacc atcgacatct ggcggagtgc cagcgctgcc ggataatcga tggcaacagg
     9481 aagctgagca gcatcccggg catggtggat cggtgccggc aggtgctgca tatcagtgtt
     9541 cactccacgg ggaaacgtcg ggcaggtttt ccacaacacg gtactttccc ggataacgga
     9601 gctggttacc tccacgcgtc cagacggtga caacaaaacc ccctccctgg ttgttttcgg
     9661 ggagcaggcg atcgcactgc tcactcagca tgtggatcac aaacggcgca tcgctgccgt
     9721 cttcaaatac cagttccagc gcatcgcggc catgcagtgc tcccttactg atgatgacgt
     9781 actcagcccc ccgcatctcc ctgagtaaat gttttgccgc atccgggaca agcagcctgg
     9841 ctgcgccggc attccagctg aggtagacat atccggcctg cgcctgtaca gactgccagt
     9901 aatccgttcc ggcgacagcc tgaccttcat tctcaataaa aaacatgggt gactcctttt
     9961 ttcttttccg ggcagggacg gtcatgcgat aacgtcatcc atcatcactg cccttaccgt
    10021 cacgccgtct gataacatca tgccgttatc atcatcacca tgataacaag catccatata
    10081 accacgccgt taaattaccg gagagccgtt tccgtcagat ccgactttcc ggaatatcac
    10141 aactttccgg aagtgtacca cgctgagaaa atcatcatat cgctgttttc cgcccctgcg
    10201 cggcgcatca caggtcaggg agatgacaca acatatagtg tcatcagcag ctgaattcag
    10261 cactacatgt tgtgctgacg ggagatggcg ctgtgcggag ggagaggaag atccgtaaca
    10321 gccggaaacc ggatctgtgc ggcagagaac gggcaggaga tatgataatt aagcagcaca
    10381 cataaaatca tatctgatat gattttcacc attaaatata aaaccatacc tgatattatc
    10441 ataaatcaat aacacacaaa tcatgcagga tgtgattttt acctttaaga acattatcag
    10501 atggagcgtg caggatggat gaagaaaggg tgttttcact cagctatgaa caactaaccc
    10561 ggtttacgga acgacgcatc cgggaatgca atctggacag ccagggcgcg atttatctct
    10621 gcgagtcagc caaagccgga gccgtgctga tcttctggca tgaactggcc ataaatggct
    10681 atgcctcaat gaatgccata aagagacagg agctcattga cgccgacttt cagcgcctca
    10741 gaaagctcat ctggccggag gatgactgga aatgactcag agccgccgac cgtcgccact
    10801 gcagcggaga gtgctgattg tgctggctgc ccttgatgaa aaacggccag gacccgtgct
    10861 gacgcgggac atagaacggg tgctggagcg aagcggggaa gccccggtct acgggccaaa
    10921 cctgcgcgcc tcctgccgtc gtctggagga tgccggctgg ctgcgtaccc tgcgcgctcc
    10981 gaacctgcag ctggcagtgg agctgacgga cgccggccgg gccgtggcac agcctctgct
    11041 gctgacagaa caggaccgcc tccgggcaga acagcgtgcg gcagaagtcg tcgtactgcc
    11101 gctggtcccg gcagcaggtc tgcccgcaga tggcacttct gccactgatc tggaggtgga
    11161 gctgaacggc ataacgtatc aggcctgccg tggggatttt gtggtgcgtc tcgacggcag
    11221 cacctgtctg cagttatgga ataaagaagg cagagtggta cgccgggagg gcgatccgct
    11281 ggaagtggcg cagtggctgc aggcctgtca tgatgccgga atagaagttc gtgtacaggt
    11341 taatgaaagc gtcactccgt aaaaacatgc cagcacggtt tacacatcac cccatggtgg
    11401 cagccggtta actttcctgc gccaccagtg gaaagaggcc gtgagcagtc cgaacaaaaa
    11461 accacccgtg atacttataa ttatggcctc acctgccacc attcctgacg gcccccggta
    11521 gataaaccac attgcacatc cccaggaaat gccccataag ctccccatca acagcgtgac
    11581 ctgccagaac ggcataaagg gtaatggcgg aagccggata cccagccgcc agagaataca
    11641 cagaagagga ggggcataat tgctccgcca catctttttg ctgtccatca gggcaatggc
    11701 ccgggctttt ttctgctcaa aggtcacagg agcacctccg ttccatgatg taaacgtgac
    11761 acagcttatt gttattgatt attattatca tgctgtgatt atgttatcac gcaccggtac
    11821 acgataacaa taaaggagaa aacagatgaa actggatata tctgtaaagt atttactgaa
    11881 gagcctgata ccaagcctca ttattcttac agttttttat ctgggatgga aagatagcca
    11941 ggaaaatgca agaatgtttt atgcgttcat cggatgcatt atcagtgcca ttacttttcc
    12001 tttttcaatg aggataatac agaaaatggt aataaggttt acagggaaag aattctggca
    12061 aaaagacttc tttacaaatc cagttggcgg aagcttaact gcaatatttg aattattctg
    12121 tttcgttata tcagttcctg tggttgccat ttacttaatt tttatattct gcaaagccct
    12181 ttcaggaaaa tgaaagggca tcattatgtg tcagatacca ataagtttat tgacctgctc
    12241 aataaactta tcattaacaa acgcactcac agccgccata ataattgcaa aacccagaat
    12301 acctacaggg gttcccagca tgacggaaaa cgtccatgct gtaactgctg aagcagctct
    12361 tcctgctgcc agtgattcaa gttttacaaa aaatggacgc cagttgtccg tttttacagc
    12421 cttttgtaac tccacagcaa catcataacg atcaataact tttccggtaa aaccaaatgc
    12481 cttactgaac agcttaaaat tctccgccat atggacctga ttaatagctt ccagggcttt
    12541 agaaatggcc atgcgatctt gtatgctata tttcttgttc agattattac ggaatttatc
    12601 aaaagcgttc agtgcatcat ccacactctt aatctgtttc ccttgagata cagaagccag
    12661 ctccttagca agttcggatg cttgtttacc gaactcatca tatatcgttc tgtagaaatc
    12721 agaggtgagt tttatggcat cctttatcat atttatttca tccctttttc ttttctcttc
    12781 agctattttc tgatttatgt cagcaagctg gctacggata ttctcttttt ccttcaacag
    12841 ggcgttaaga gcatcatttg catgcttttg ttcatttgtt ctggcactga cgttatttct
    12901 cgccgaattt accgcagatt cagcagaggt gattttattt ctggcatcaa gcaatcgttg
    12961 ccgtaactta tcccattcag caacttcagc attataaatt ttcttgtcac cttccacatc
    13021 ctctgtcacc accggattcc ggtcattcag aatattgcgt ttatagtcca gaccggaatg
    13081 ggtcagaaga taattcaggt ttgcacggtt atcgatgacc gcgctggttg tgatttttat
    13141 cgtaccggca aatctcggat caccactcac aacaatagaa tgatttgttg atgaacgccc
    13201 cgggaatttt tcaggggaaa ctatcgtttt gccgtcaggc gtgtttttct gttgcgtcag
    13261 ctgttgttca gctctggtta atgcattacg ggccgtctta agcgattccg tcaccgaact
    13321 gagtttattc cgggcctctg aaatggccct gtcattccgt gtctgctgcc cgagcagacg
    13381 ggcctgttgt gcatcaagaa cggcaagggc tgccccgcca tttttttcca gctctgacaa
    13441 ccgcgtgtcc agaatacctt ttctgcgctc gtattcagca agcatatcag cggccttctt
    13501 gtctgcgttc tgcgcatcct tctgccgttt ttcagcctgt tcgattaacg acctggcctc
    13561 gcggggagat atacgaagag aatcagcatc agcaaacagc atccggctct ctgtatgcag
    13621 ccggagggca tcatatccgg cgatttcagt acggaatccg tattctctca gctctgcctg
    13681 caggagtctg aactcctgtc gtgtaatatc agcgacatct gcgtttccgg cacggagtgt
    13741 tttcagtgtg ttttcatctt tttcccttgc agcaattgcc gcagaaaggc gttttccggc
    13801 ttcagtacgc ttattctcat tctctttctg ctgcacgctg agcgtgtttt tgtacgctgt
    13861 gatttccttg tccctttttt cgatatcact tcgggttggg gaatcatcga cccactcgtt
    13921 tccgccccag atggttttgt tcccgaagga actccatgca ggcggggtac catgaaaaac
    13981 atcgacacgt ggagggttta cataaatatc aacggccata atttcatggc catctgaatc
    14041 ataccccagc gattctgcac cgggatttgt aatacgtaca gggtcagaca ttcggtatct
    14101 ccttcatcct ctgaggaaaa caaatgaatt aactgcttac gtatatggat acatatacag
    14161 tattaattta ttaaggagaa aatggcatgt caagatttaa tgatagtgat tatcattaac
    14221 atgaagtggt gttaaaccgt tcagaaagcc tgctgacgtt gtggcagcat cacagtctgg
    14281 gtattctctg gtgcaataca aaaaccggac tttcccgtaa caacagacaa tgttacggga
    14341 aaagttacag aactgagcca caaaatatct cagtacctga gatgcataaa aacaccgtac
    14401 atatgtttct caaaaggggg atgatcgtta tccagccatt tcggtggatc ggtatgcaga
    14461 acaccaatct gcttcatgcc ggtatgctgc ctggattttc tcgcgcagta atcactgtct
    14521 tcatatccca tcgtttcgat gaccactctg gccgtctttc cgtcaggagc ccttgctgta
    14581 acaatgaaat ccggcagtac atatttcttt tcaccattaa cataaacttc tgtatcaaac
    14641 aggggccttt ccagacttaa ttccggcgta cctggctttc ttttaagcca gctggccaca
    14701 ttcagaagag cagtgagcgt gtttcgttcc agtttgctgt ccacaggcac cggacatgtc
    14761 agttgataaa gagcatgagc atacccttca ctgcagatga ttctgccatc ggcactccgg
    14821 caaaaagcaa gaataaccca gtaaggggga cgtattccgc cctgcgcttc accattaata
    14881 ctgatccccc gcacaggtct gatgacctga atattaccgt tcgcccagtg aatttccaca
    14941 gcatcacgac agatgtgctg cgccataaaa atctgaaaga acatatgctt tcttctggca
    15001 ggccagtgtc gttctgaatt ctccagcgtt tccattaacc gctcctgaga cctttcagac
    15061 attccaggcc ggaaatggat aatttcagat aaggatcttc cccgaatata ctcctgctgc
    15121 agtgtaacct gccgtatggc atcaagacac tcacgataat tagtccggat acggggatta
    15181 gccagggaat ccagtttatt cagcccagca tcgtcaataa gagacaggag taaacgggca
    15241 aggcgcagac gtttcttgcg aacggaggag ctctcaccat tatggtaagc agagcgggcc
    15301 tgcagtctga taacagaatt actgtcatca ggtggtaaaa agtttctgta atttatacgg
    15361 gtgctaacgg gatgccggct tgctccggaa gatgtttcct cttcatttcc ggataactga
    15421 cgatacatgg ggcattcagg tgcatgctca tgattaaaac tggacagata aagagtaccc
    15481 gtattgtttt taagagcagc aaaattcatg gccggcgaat ctcccggaat acagtcacac
    15541 tgtaaccatg catcatactc acgcatttcc cggagaatat cattggccag gttatgtagt
    15601 gccatggttg ctggcttttt cagtccacag accgtcttta ctgcagtctc ttcctcactg
    15661 ctaagctccc gaatcacttt gccccgggga ttcttcttta caagtatcat tggcataatg
    15721 aaagcactcc ttatgtacat gcttaaacag taagtaaaat atacaaagaa caccccatga
    15781 aacaaaggca gagatgaact gtgcgccccg atatcttctt ctgatatcct ttcaaatgaa
    15841 ttgtttttat ttatatcatg acaacattaa agtgagagaa tatatcaacc atgtttctgt
    15901 cagggttcag tagaatatca attcgactgt cacgccataa ctgttactac ctcgagtata
    15961 aatattcaga gaacggactt attagccatg aaaaatcacc tgatgggaaa tttactaatt
    16021 tatgaattgt gatattatta aagagtaaaa ccagccagcc gaggtattta tgggaaaaaa
    16081 tcaggaagaa aggaaaactc ccgtcattgt ggtgaaaaaa cgtcgtactt tttcgctacc
    16141 gtccttatcc gaaaaaacag atattatcgc acctgtattt actgaacaga cggcagaatc
    16201 agcccccgcc ggaataaatt catctgccgt cgaaacacat atccccgaag cgccagccag
    16261 gaagaagaaa aagaaaagac accgttttcc acgtccctcc cactggaccc gtgagtatac
    16321 tcacgagtgc gtggaaaaaa taaaggccct gtttcctcat cttcgtgctg aaggtggtgg
    16381 atttattccc ctgaaaattg gcataaacaa tgatatttcg gcatttctgg cagaacaccc
    16441 ggagacagaa ctgaccatgg acgaatggct ctgtgcagtg tcctgcatca catcccgccg
    16501 cgtatatctg cagcggacag ctgtcgccgg cgtcccacgt tatggcctgg atggtcaccc
    16561 caaagggcag gtctcagact ctgaagcaca gagtgccggt cgcagactgg cgacattaga
    16621 acaaaagtgg ctcaggatgc aggcacagca ggagaatatt tctgggcaat aaacactcct
    16681 gataacaggt ggacattacg tatcagaaaa aatgggaaga gaatgttatg tgtgattcag
    16741 aagcggagaa gattttattc atcctgaata aacatgaacg ggcatacaga cgcctgatcc
    16801 ccgtatatat ttttttagct atagtattat ggattttacc tattctcctg attgtatacc
    16861 tgtatttctg tatagttgac aatgagtttc tcagaatcgg tttctgtatt gcatggacag
    16921 catactactt tctttcgctt ttcaagtatt ttgtaaaata cttctttcct ctcgatcgtg
    16981 aagatttgct taaactgatg gagctcgcca aagattctcc tgatgcgaaa caggagcttc
    17041 ttgagcgtct tttgtccgga aaaagaatga ccgggcggga tgaaatggat atcaagcgca
    17101 ttctaaaaac aaaggaagca cttgataaca tcaggaattt tattgataaa cagcagttaa
    17161 cgaatagtga tactccgaag aaaagcacgg tcccaaatga agataaaaaa ggcataagat
    17221 gatgatcaca ggaaatggaa taaacactgt aactgtcaat ggaaaagtga aacacatcac
    17281 agaactggat gatataacac tttgtttaga gtggacaaaa ctacgagagg aaaacaatcg
    17341 tctttatgaa attaataacc aggcaaacag aggatggcgt ggtttcatcc taagattgat
    17401 aggggttaac ctgccagata aaaggactga gtttacacaa cgtctcttat tgacgcgcaa
    17461 aatttcgggt tcggtcatga aaaagtaaca aaaccgcgca acagacataa atatgacagt
    17521 agccatgaaa aagcagagag agacagggtg atacagaaaa gtaactattt tttagctata
    17581 gtattattgg ttttacctat ttttgtgatt ttatttctgt atatttgaca atgagtctct
    17641 cagaatcggt ttctcgaagt gacgagcgat cagaatggag acaaataaat tcgggaaacg
    17701 aaccttcacg gtaagttttg tcacatttat catccctaaa catgagaatt atcaccaaaa
    17761 ctaaccaccc aaccaacagc agcgccggga gcagcattat aaaatcatgt acagagttgc
    17821 gtgacgacat gataagttct cattctcaat atgttcttta ccagattata ccagtgatac
    17881 tttaacgcac catcattaat gctaatgtat gcatacatta gcattaatga tgaaggagaa
    17941 gaacaacaat ggcagtcaac ccacttattt tttggatttc ttaattttcg tctgataaag
    18001 ccgctttacc cgttcaattt ctgccagcgt tgcggaccag tgtagttcgg cggcatgacg
    18061 atatctggca gccatttttt ccaccatctc cggattgctg tccgcgctgc ggtgctgacg
    18121 ccccttctcc ccaaaaactt caacaaccag ccactggctt ccggttctgc cgaacaatgc
    18181 cttgcacaaa cgtctcacag tcagcggggt taaatctgga tcgggatcgt aggcaatggt
    18241 agcaaaaata aagcgcagca tttctgatgt cttgtaacgc tttacgctgg cagccaggcg
    18301 tgaggcacgt tgcggtgctt cccagcggtc gttactgtaa ttagccactt cagccagggg
    18361 tcgtctcaga aaacggaaaa taaagcacgc taagccggtt gcagaggccg tagcggcctg
    18421 aacttccccg cgccgatctt ggcgctgctg cgccataggt aatcaccggt caggttgatg
    18481 tgctcccagc cgagtggcga caggtactgc aatagcgagt catcgacggc atgaccattg
    18541 ccgcgcaacg catgcgccgc acgctccagg tagaccgtgt tccacagcac gatggccgcc
    18601 gtcaccaggt tgaggccgct ggcccggtag cgctgctgct cgaaactgcg gtcacggatt
    18661 tcaccaaggc ggttgaagaa cacggcacgg gccagcgcat tgcgcgcctc gcccttgttc
    18721 agcccggcat gcacgcggcg gcgtagctcg acgctttgca gccagtcgag gatgaacagc
    18781 gtgcgctcga tgcggcccaa ctcgcgcagc gcgacggcca agccgttctg gcgcgggtag
    18841 ctgccgagtt tcctgagcat cagcgaggcc gtcaccgtgc cctgcttgat cgaggtggcc
    18901 agccgcagga tttcgtccca atgggcgcgg acgtgcttga tgttgagcgt gccgccgatc
    18961 atcggcttga gcgcgtcata ggcggcatcg cccttcggga tgtagagctt ggtgtcgccc
    19021 aggtcgcgga tgcgcggcgc gaagcggaag cccaagaggt gcatcagggc gaagacgtga
    19081 tcggtgaagc ccgccgtgtc ggtgtagtgc tcctcgatcc gcaggtcgga ttcgtggtac
    19141 agcaggccgt cgagcacgta ggttgagtcg cgcaggccga cattgaccac cttggtgtgg
    19201 aatggcgcgt attggtcgga gatgtgggtg tagaaagtcc gtcctgggct gctgccatat
    19261 tttgggttga tgtgccccgt gctctttgcc ttgctagcgg ttcggaaatt ctgtccgtcc
    19321 gatgatgatg tggtgccatc gccccagtgc ccggcaaagg gatgccgaaa ctgagcgttg
    19381 accagttcag ccaacgctgt cgagtacgtt tcgtcgcggg tatgccaggc ttgcagccaa
    19441 gcgagcttcg cgtaggtcgt gccggggcag gactcggcca tcttggtcag gcccaggttg
    19501 atcgcgtcgg ccaggatcgt ggtcaacaac aggttcttgt ccttggccag atcgcccgat
    19561 ttcaagtgcg tgaagtgccg ggtgaagccc gtccactcat cgacttcgag cagcagttcg
    19621 gtgatcttga cgtgcggcag gaccatggct gtctggtcta tcagcgcctg cgcggtgtcg
    19681 ggcaccgccg catccagcgg cgtgatcttc aagcccgact cggtgatgat ggcatccggc
    19741 aggtcgttgg ctgccgccat gcggttgacg gtggcaagtt gtgcttccag cagcgtcagc
    19801 cgctcatgca gatattgttc gcagtcggtg gccacggcca gcggcaattc gctggactgc
    19861 ttgaggctgg tgaacttctc gggcggtacc aggtagtcct cgaagtcctt gaactggcgt
    19921 gaaccctgca cccagatgtc gcccgagcgc agggagttct tcaactcgga cagcgcgcac
    19981 agttcgtagt agcgccggtc gatgccggcg tcggtcatca ccagtttctg ccagcgcggc
    20041 ttgatgaagc cggtcggtgc atcggctggc agcttgcggg cgttgtcggt gttcatgccg
    20101 cgcagcacct caatggcatc aagcacgttt ttggcggcgg gcgcggcccg cagcttgagc
    20161 acggcaagga attccggtgc atagcggcgc agggtggcgt agctctcgcc gatgcgatgc
    20221 aggaaatcga agtcatcggg ttgcgcgagc ttctgcgcct cggtgacgct ctcggcaaag
    20281 gaatcccagg acatgacggc ctcgatggcg gcaaacgcat cgcggcctga ttgcttggcg
    20341 tcgatcagcg cctgaccgat gcgcccgtac agacgtacct tggcgttgat ggccttgcct
    20401 gacgcctgga actgctgctg atgcttattc ttggcagcgt taaacagctt acccaggatg
    20461 cggtcgtgca ggtcgatgat ttcgtcggtg acggtggcca tgccctcggt ggccagcgcc
    20521 acgagagtgg cgtagcgccg ttgcggctcg aatttggcca ggtcggcggg tgtcatctgg
    20581 ccgccctcgc gggcaatctt gagcaggcgg ttctggtgaa ccagccgctc gatgccggta
    20641 ggcagatcga gtgcctgcca tgccttgagg cgttcgatgt gttccagcat atgccgcgaa
    20701 tttggcttgg ccggagactg gcgcaaccaa gccaaccagg tcgtcttgcc gttgtcccgg
    20761 cgcttgagca gatcgtcgag gcggcggcga tgcgcgtccg ccagtggttc ggccaaggcg
    20821 tcgtagatgc gccggttagc acgggtgatc gcctcggcac tcgcccgctc gacggcgttg
    20881 agggcgggca gaatgaccga ctgccgccgc aggtgcccga tcaaggcgct ggccagcacg
    20941 atgcctttgt cggtttgcat cgccagctcg gtcagcatct ggacggcctg ccggtaatgg
    21001 ctcatggtga agggccggaa accgaacacg gtttgcagct cgctcaggtg ctcgcgc
//
LOCUS       DAZGCS010000082        20822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGCS010000082 DAZGCS010000000
VERSION     DAZGCS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.082"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..846
                     /gene="exuR"
                     /locus_tag="ACHK01_003362"
     CDS             70..846
                     /gene="exuR"
                     /locus_tag="ACHK01_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO8098270.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1086..1169
                     /gene="yqiM"
                     /locus_tag="ACHK01_003363"
     CDS             1086..1169
                     /gene="yqiM"
                     /locus_tag="ACHK01_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO8098271.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1191..1853
                     /gene="yqjA"
                     /locus_tag="ACHK01_003364"
     CDS             1191..1853
                     /gene="yqjA"
                     /locus_tag="ACHK01_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO8098272.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1857..2240
                     /gene="mzrA"
                     /locus_tag="ACHK01_003365"
     CDS             1857..2240
                     /gene="mzrA"
                     /locus_tag="ACHK01_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO8098273.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2387..2755
                     /gene="yqjC"
                     /locus_tag="ACHK01_003366"
     CDS             2387..2755
                     /gene="yqjC"
                     /locus_tag="ACHK01_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO8098274.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2793..3098
                     /gene="yqjD"
                     /locus_tag="ACHK01_003367"
     CDS             2793..3098
                     /gene="yqjD"
                     /locus_tag="ACHK01_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO8098275.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3101..3505
                     /gene="yqjE"
                     /locus_tag="ACHK01_003368"
     CDS             3101..3505
                     /gene="yqjE"
                     /locus_tag="ACHK01_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8098276.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3495..3794
                     /gene="yqjK"
                     /locus_tag="ACHK01_003369"
     CDS             3495..3794
                     /gene="yqjK"
                     /locus_tag="ACHK01_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO8098277.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3980..4372
                     /gene="yqjF"
                     /locus_tag="ACHK01_003370"
     CDS             3980..4372
                     /gene="yqjF"
                     /locus_tag="ACHK01_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8098278.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4442..5428
                     /gene="yqjG"
                     /locus_tag="ACHK01_003371"
     CDS             4442..5428
                     /gene="yqjG"
                     /locus_tag="ACHK01_003371"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO8098279.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5721..6086
                     /gene="yhaH"
                     /locus_tag="ACHK01_003372"
     CDS             5721..6086
                     /gene="yhaH"
                     /locus_tag="ACHK01_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8098280.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6328..6684
                     /locus_tag="ACHK01_003373"
     CDS             6328..6684
                     /locus_tag="ACHK01_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8098281.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6735..7631)
                     /gene="yhaJ"
                     /locus_tag="ACHK01_003374"
     CDS             complement(6735..7631)
                     /gene="yhaJ"
                     /locus_tag="ACHK01_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8098282.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7736..8437
                     /gene="yhaK"
                     /locus_tag="ACHK01_003375"
     CDS             7736..8437
                     /gene="yhaK"
                     /locus_tag="ACHK01_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO8098283.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8460..8624
                     /gene="yhaL"
                     /locus_tag="ACHK01_003376"
     CDS             8460..8624
                     /gene="yhaL"
                     /locus_tag="ACHK01_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO8098284.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8836..10146)
                     /gene="cyuA"
                     /locus_tag="ACHK01_003377"
     CDS             complement(8836..10146)
                     /gene="cyuA"
                     /locus_tag="ACHK01_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO8098285.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10174..11505)
                     /gene="cyuP"
                     /locus_tag="ACHK01_003378"
     CDS             complement(10174..11505)
                     /gene="cyuP"
                     /locus_tag="ACHK01_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8098286.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11780..13144)
                     /gene="tdcG"
                     /locus_tag="ACHK01_003379"
     CDS             complement(11780..13144)
                     /gene="tdcG"
                     /locus_tag="ACHK01_003379"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8098287.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13216..13605)
                     /gene="tdcF"
                     /locus_tag="ACHK01_003380"
     CDS             complement(13216..13605)
                     /gene="tdcF"
                     /locus_tag="ACHK01_003380"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO8098288.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13619..15913)
                     /gene="tdcE"
                     /locus_tag="ACHK01_003381"
     CDS             complement(13619..15913)
                     /gene="tdcE"
                     /locus_tag="ACHK01_003381"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO8098289.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15947..17155)
                     /gene="tdcD"
                     /locus_tag="ACHK01_003382"
     CDS             complement(15947..17155)
                     /gene="tdcD"
                     /locus_tag="ACHK01_003382"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO8098290.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17181..18512)
                     /gene="tdcC"
                     /locus_tag="ACHK01_003383"
     CDS             complement(17181..18512)
                     /gene="tdcC"
                     /locus_tag="ACHK01_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO8098291.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18534..19523)
                     /gene="tdcB"
                     /locus_tag="ACHK01_003384"
     CDS             complement(18534..19523)
                     /gene="tdcB"
                     /locus_tag="ACHK01_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO8098292.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKNVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19622..20560)
                     /gene="tdcA"
                     /locus_tag="ACHK01_003385"
     CDS             complement(19622..20560)
                     /gene="tdcA"
                     /locus_tag="ACHK01_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO8098293.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESGFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5381 a         5422 c         4948 g         5071 t
ORIGIN      
        1 tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat tccactccgg
       61 gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc cgctgacctg
      121 aaagagcgca tcgaacaggg cgtctatctg gtaggtgata aactgcctgc agaacgcttt
      181 attgccgatg aaaagaacgt cagccgtacg gtagttcgtg aagccatcat catgctggaa
      241 gttgaaggct atgtcgaagt gcgtaaaggt tcaggtattc atgtggtttc caaccagccg
      301 cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc gtttgagttg
      361 cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac tcaggtaacg
      421 aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga acaatgcttc
      481 cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac gcagaactcc
      541 gccctggcgg ccatcgttga aaaaatgtgg acccaacgta gccataaccc gtactggaaa
      601 aaactgcacg aacacattga ttcccgtacc gtcgataact ggtgtgatga ccacgatcaa
      661 atcctcaagg cgctgattcg caaagatcct catgctgcta agctggcaat gtggcagcac
      721 ctggaaaaca ccaagatcat gttattcaac gaaaccagcg acgacttcga gttcaatgcc
      781 gaccgctatc tgttcgccga aaacccggta gtgcatctcg atacggccac cagtggcagt
      841 aaatgaaact cctgtccgac aggcgctgcg acgcgcctgt ttgtctcgtc ggtaagcgaa
      901 aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat tcagcaaaat
      961 cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt acaattagat
     1021 tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag ggaatagttc
     1081 aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac attgcggtag
     1141 tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga atggaacttt
     1201 tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc aatccatcga
     1261 tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac ggcttgcttc
     1321 cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg attgcgaaag
     1381 gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc ctcggctgct
     1441 gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa aactggctat
     1501 ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa cacggtttat
     1561 cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg acgattgccg
     1621 ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc ggtctgctgt
     1681 gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta tttttaaagt
     1741 acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg gtgtttggcc
     1801 tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg taagggatgc
     1861 aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta cttctggtcg
     1921 gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg gcgattcgtg
     1981 ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac cttgacgctc
     2041 atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt acatttgact
     2101 ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc catggctaca
     2161 tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg ttacgggata
     2221 actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact ttggtgattt
     2281 caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg ttgtctggac
     2341 aatcgttcct ttgtaatagg tccgaatcac aatggaaggt tcaagaatga aataccgcat
     2401 cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta ccctgtgtca
     2461 ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc aaaaccagaa
     2521 tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt cagatagcca
     2581 gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg cggaacgcca
     2641 gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca aacgcgaacg
     2701 gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact actaactcac
     2761 aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact acggaacatc
     2821 tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc tcatctggcg
     2881 agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca ctgaaacaga
     2941 gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt gtcgcggcgg
     3001 cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt ggcgctgcaa
     3061 tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca ctcatcacgc
     3121 gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca tcatggttga
     3181 aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa aagctaatct
     3241 ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg gtcttatgag
     3301 cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg cgatgattgc
     3361 caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt tacgtaaatc
     3421 gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc ggcaactgct
     3481 cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg caactgctta
     3541 gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg ctggaggcaa
     3601 caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg gcgctggttg
     3661 gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc cgctgggcca
     3721 gacgcggttt tggcgtatgg agcgcctggc gtctggtaaa aacgaccctc aagcagcaac
     3781 agcttcgcgg ttaacttccc tctggccgga gcaattccgg ccttatccct caaatttttt
     3841 gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta tgattctctc
     3901 catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca gcagcctgag
     3961 tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgcat
     4021 tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt actctggtac
     4081 ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg tgattctgct
     4141 tgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca cagccctgtt
     4201 tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg ctgaaggcgt
     4261 caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc tgctggcaat
     4321 taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc
     4381 tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg aggagagaaa
     4441 aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca aatctaccgg
     4501 cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg atggcgcacc
     4561 tggccccact ggcacaggcg gttttatcgc agagaaagat cgttatcatc tctatgtttc
     4621 actcgcctgc ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag gactggaacc
     4681 gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga cctttgatga
     4741 cagttttccg ggagcaaccg gagatacgct ctatcaacat gaatttctgt atcagcttta
     4801 tctccacgcc gatccacact acagcggacg agttactgtt cccgtgctgt gggacaaaaa
     4861 gaaccacacc atcgtcagca acgaatcagc agaaatcatc cgcatgttta ataccgcgtt
     4921 tgatgcgctg ggcgcgaaag cgggtgatta ctacccacca gccctgcaaa cgaaaattga
     4981 cgaacttaac ggctggattt atgacaccgt taacaacggc gtgtataaag ctggttttgc
     5041 caccagccag caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc tggcgcgact
     5101 ggagcagatt ttaggccagc atcgttacct gaccggcaac cagctaaccg aagccgatat
     5161 tcgcctgtgg accacgctgg tccgttttga tccagtgtat gtgacccact tcaagtgtga
     5221 taagcaccgc atcagcgatt accttaatct gtatggcttc ctgcgtgata tctaccagat
     5281 gccgggaatt gccgaaacag tcaatttcga tcacatccgt aatcattact tccgcagcca
     5341 taagaccatc aaccctacgg ggattatttc aattggtcca tggcaggatc tcgatgaacc
     5401 gcatggacga gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc ctctgataat
     5461 tagctaagtg catgttgttc atgtcggatg cggcgtgaac gccttatcaa acctacaaaa
     5521 catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc gcatctggcg
     5581 attttgcgct tgtcatcagt cttagcgcgc cttcacagca ctttattaat tctttgaacg
     5641 aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa aacaagtgat
     5701 tgagcaaaat tgaggcaaaa atggactggt atctgaaagt actaaaaaat tatgtcggtt
     5761 tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac atcatcttta
     5821 cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt ggcgaaggca
     5881 tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt cagttccgcc
     5941 gcctgcacga caccgaccgc tcggcgtggt gggcactgtt gttcttaatc ccgtttatcg
     6001 gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa aaccgctttg
     6061 gaccagatcc gaaactggaa ccttaaaaca aatctgctcg ccggatgtct gactcatccg
     6121 gcttttatca tcatgttgat gaatgaataa tttactaatg tttatttaaa atatttcaat
     6181 gtcgttatat aaattaccta taaaaaacaa ccatgtattt atcttaaata tcaaaacctt
     6241 tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga atagactttc
     6301 gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt aaaaaattat
     6361 gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct gattaacgcc
     6421 atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt tccattcctt
     6481 tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt tcgtcgtctg
     6541 cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat cattggctgg
     6601 ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg ttacggaaac
     6661 gatcctaagt ttggttcaaa ttaatcttag aattggggcg acatttcgcc cctttttatt
     6721 aataataaca aatattattt tccgctaaaa agtttgggaa tttcccgcag acaccaggat
     6781 ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc gctggtcgat
     6841 tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat cggatacggc
     6901 atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat cgtgctcacc
     6961 gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc acgagcggta
     7021 tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg ttccggctcc
     7081 tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaaggtata gagcttgcgc
     7141 gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacaat atccgcccgc
     7201 ccctgctcca gtcgttccca cgcccccgcc agcacttcgg tgatgattgc cagttgggta
     7261 ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc
     7321 tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc
     7381 agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct cccgacattg
     7441 gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc ttcttccagt
     7501 ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc cgccgccgca
     7561 aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt taatgccctt
     7621 tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa tatctggcta
     7681 acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa gaattatgat
     7741 tactacccga actgccagac agtgtggaca agcagactac ggatggttgc aggcccggta
     7801 tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt
     7861 gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt
     7921 cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg aaggcaatca
     7981 tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg tcagctacag
     8041 cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc tggacgcctg
     8101 cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca agcagcaatt
     8161 aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag tgtggctgca
     8221 ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta
     8281 tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga aagcggcgct
     8341 gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg ttgccgattc
     8401 cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg gagatgatga
     8461 tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg
     8521 ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa gccatcgtcg
     8581 cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag gttgccggat
     8641 gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat taaacaaccc
     8701 gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac acaacaacat
     8761 caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc cggcacaata
     8821 ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     8881 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     8941 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     9001 ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca
     9061 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataacg
     9121 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     9181 aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat
     9241 cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg
     9301 cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg gaagcgtagc
     9361 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     9421 tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     9481 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat cgagaataaa
     9541 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     9601 ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     9661 caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac
     9721 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     9781 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     9841 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     9901 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     9961 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
    10021 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
    10081 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
    10141 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
    10201 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
    10261 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg
    10321 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag
    10381 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
    10441 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata
    10501 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
    10561 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
    10621 ctcataggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa
    10681 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
    10741 cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
    10801 gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
    10861 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
    10921 cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt tgaataacaa
    10981 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
    11041 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag cactatcgtt
    11101 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc
    11161 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
    11221 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
    11281 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
    11341 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
    11401 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
    11461 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
    11521 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
    11581 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
    11641 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
    11701 atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
    11761 atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct ccgcgtgatg
    11821 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
    11881 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt
    11941 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggat
    12001 cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
    12061 gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc acgccaatct
    12121 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
    12181 tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
    12241 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
    12301 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
    12361 cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagctga cgacgcagcg
    12421 ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
    12481 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca atttccgctt
    12541 tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg ccgttgtaat
    12601 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac gtttcgacat
    12661 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
    12721 ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg ttctcatggc
    12781 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
    12841 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
    12901 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg gcgacatccg
    12961 tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca atatggctcg
    13021 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
    13081 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
    13141 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt
    13201 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
    13261 tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct gatgctcatc
    13321 gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg tgatgaacac
    13381 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
    13441 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgcgggca
    13501 aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct gaacataagg
    13561 gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
    13621 agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt
    13681 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
    13741 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
    13801 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
    13861 ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag atcccatctt
    13921 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
    13981 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
    14041 gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
    14101 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag cgttcaacca
    14161 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
    14221 cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg cgggcatatt
    14281 tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat gccatagtgc
    14341 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
    14401 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
    14461 cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg
    14521 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt
    14581 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
    14641 cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt
    14701 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
    14761 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc atagtgtgca
    14821 gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
    14881 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggtg
    14941 tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt tcctgtgcct
    15001 gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcg aggaacgatg
    15061 ccgtgcggcc cagcgacatc gcgccgccat tttgcgattt cactgccgcc agataagcga
    15121 agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata tcaaagccat
    15181 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
    15241 gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg agatcggcaa
    15301 attgcagttc gcgttcacgt accagataac tgatgccata cagcgctacg cggcgatagt
    15361 caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg ccagatttac
    15421 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
    15481 gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg tggaatgaac
    15541 ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt
    15601 gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
    15661 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcattttcg atacggatgc
    15721 cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt
    15781 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgtttt
    15841 tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa
    15901 tatctacctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
    15961 cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt tcttcgttag
    16021 tcggaataac ggcacaaatg acacgcgcat tttcactgga aacaattcgc tcaccacagg
    16081 agttagagcg attattcatt tctgtatcaa tctctacgcc taatacagcc aaatgttcca
    16141 tgaccagacg acgaattaag cttgaattct ctcctattcc gccggtgaat ataattccat
    16201 ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg
    16261 ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
    16321 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
    16381 ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca aagtcgacat
    16441 cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt gaagtatcaa
    16501 cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc gccacaacca
    16561 ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa acatagcggt
    16621 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
    16681 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aataccgcca
    16741 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa ttggcgtaat
    16801 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    16861 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacac
    16921 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    16981 tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    17041 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agtacggaaa
    17101 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    17161 ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga
    17221 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    17281 ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    17341 gcttcaataa ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    17401 atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt gtcgccttta
    17461 taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata gtgaccgaag
    17521 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    17581 aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    17641 atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaacag gcagctaaag
    17701 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    17761 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt agaaaccacg
    17821 aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat ccccagccag
    17881 acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    17941 ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag gctggcgata
    18001 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    18061 aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg
    18121 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    18181 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    18241 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acacagacgc
    18301 gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat cagaagaatc
    18361 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    18421 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgttttgtc
    18481 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    18541 tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    18601 ctgacgtttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    18661 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    18721 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    18781 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    18841 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt
    18901 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    18961 aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    19021 tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg tgggataaaa
    19081 atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaaattatca
    19141 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga ttttggcgca
    19201 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag cgaaacccct
    19261 tgcgcatggt tgcccgcaga acaggccact acacctttac gtttttccgc atcaatcagt
    19321 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    19381 aacttcagga atatttcacc tttgcaacgt tcactaaaat agtttgagcg aggcatgcct
    19441 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc
    19501 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    19561 ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata aaaacaaata
    19621 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    19681 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    19741 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga tttgaaccaa
    19801 aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    19861 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    19921 acgtagtaag aagctcgctg tagtacccca tattggtttg tggcaacacc cactgttcgt
    19981 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccaaca
    20041 caaacccgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcactta
    20101 acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    20161 gcgcttcata catagaaact tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    20221 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    20281 cagaagacat accacttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    20341 aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt ttccgcacca
    20401 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gctggttgag
    20461 ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact tcctgaaaga
    20521 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    20581 tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga ccactcacaa
    20641 attaactttc aatactttcc agggtatcgt tattacaatt aaacaccttt aaatatcaac
    20701 aagttaaagc ataaaaatca gcataaaccc tgatttttgt caaaaaaaat gacggggata
    20761 accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa ggggctgaga
    20821 cc
//
LOCUS       DAZGCS010000083        20609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGCS010000083 DAZGCS010000000
VERSION     DAZGCS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.083"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1134
                     /gene="phoE"
                     /locus_tag="ACHK01_003386"
     CDS             79..1134
                     /gene="phoE"
                     /locus_tag="ACHK01_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8098294.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1173..1574)
                     /gene="crl"
                     /locus_tag="ACHK01_003387"
     CDS             complement(1173..1574)
                     /gene="crl"
                     /locus_tag="ACHK01_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8098295.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1632..2876)
                     /gene="frsA"
                     /locus_tag="ACHK01_003388"
     CDS             complement(1632..2876)
                     /gene="frsA"
                     /locus_tag="ACHK01_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8098296.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2968..3426)
                     /gene="gpt"
                     /locus_tag="ACHK01_003389"
     CDS             complement(2968..3426)
                     /gene="gpt"
                     /locus_tag="ACHK01_003389"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8098297.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3687..5144
                     /gene="pepD"
                     /locus_tag="ACHK01_003390"
     CDS             3687..5144
                     /gene="pepD"
                     /locus_tag="ACHK01_003390"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8098298.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5201..5815)
                     /gene="prfH"
                     /locus_tag="ACHK01_003391"
     CDS             complement(5201..5815)
                     /gene="prfH"
                     /locus_tag="ACHK01_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO8098299.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5812..6951)
                     /locus_tag="ACHK01_003392"
     CDS             complement(5812..6951)
                     /locus_tag="ACHK01_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO8098300.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7197..7649)
                     /gene="yafP"
                     /locus_tag="ACHK01_003393"
     CDS             complement(7197..7649)
                     /gene="yafP"
                     /locus_tag="ACHK01_003393"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8098301.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7646..8701)
                     /gene="dinB"
                     /locus_tag="ACHK01_003394"
     CDS             complement(7646..8701)
                     /gene="dinB"
                     /locus_tag="ACHK01_003394"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO8098302.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8772..9557)
                     /gene="lafU"
                     /locus_tag="ACHK01_003395"
     CDS             complement(8772..9557)
                     /gene="lafU"
                     /locus_tag="ACHK01_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO8098303.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPKSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9529..11241
                     /locus_tag="ACHK01_003396"
                     /pseudo
     CDS             9529..11241
                     /locus_tag="ACHK01_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11346..11624
                     /locus_tag="ACHK01_003397"
     CDS             11346..11624
                     /locus_tag="ACHK01_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO8098304.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            11617..11973
                     /locus_tag="ACHK01_003398"
     CDS             11617..11973
                     /locus_tag="ACHK01_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO8098305.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(12030..12803)
                     /gene="yafL"
                     /locus_tag="ACHK01_003399"
     CDS             complement(12030..12803)
                     /gene="yafL"
                     /locus_tag="ACHK01_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8098306.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGEIIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12989..13249
                     /gene="dinJ"
                     /locus_tag="ACHK01_003400"
     CDS             12989..13249
                     /gene="dinJ"
                     /locus_tag="ACHK01_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO8098307.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            13252..13530
                     /gene="yafQ"
                     /locus_tag="ACHK01_003401"
     CDS             13252..13530
                     /gene="yafQ"
                     /locus_tag="ACHK01_003401"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO8098308.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            13686..14426
                     /gene="dpaA"
                     /locus_tag="ACHK01_003402"
     CDS             13686..14426
                     /gene="dpaA"
                     /locus_tag="ACHK01_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO8098309.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14397..15164)
                     /gene="yafJ"
                     /locus_tag="ACHK01_003403"
     CDS             complement(14397..15164)
                     /gene="yafJ"
                     /locus_tag="ACHK01_003403"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO8098310.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(15370..15948)
                     /gene="lpcA"
                     /locus_tag="ACHK01_003404"
     CDS             complement(15370..15948)
                     /gene="lpcA"
                     /locus_tag="ACHK01_003404"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO8098311.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16188..18632
                     /gene="fadE"
                     /locus_tag="ACHK01_003405"
     CDS             16188..18632
                     /gene="fadE"
                     /locus_tag="ACHK01_003405"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO8098312.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(18675..19121)
                     /gene="ivy"
                     /locus_tag="ACHK01_003406"
     CDS             complement(18675..19121)
                     /gene="ivy"
                     /locus_tag="ACHK01_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO8098313.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19302..20069
                     /gene="yafV"
                     /locus_tag="ACHK01_003407"
     CDS             19302..20069
                     /gene="yafV"
                     /locus_tag="ACHK01_003407"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO8098314.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(20190..20285)
                     /locus_tag="ACHK01_003408"
                     /pseudo
     CDS             complement(20190..20285)
                     /locus_tag="ACHK01_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20293..>20609)
                     /locus_tag="ACHK01_003409"
     CDS             complement(20293..>20609)
                     /locus_tag="ACHK01_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8098315.1"
                     /translation="KEPEMTVRYYISSADLTAEKFATAIRNHWHLENKLHWRLDVVMN
                     EDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
BASE COUNT         5048 a         5257 c         5280 g         5024 t
ORIGIN      
        1 ctcaggttaa aaactttcct gttttcaacg gggctctccc gctgaatatt cgcgcgttaa
       61 ttaaaatcag gaatgaaaat gaaaaagagc actctggcat tagtggtgat gggcattgtg
      121 gcatctgcat ccgtacaggc cgcagaaata tataacaaag acggtaataa actggatgtc
      181 tatggcaaag ttaaagccat gcattatatg agtgataacg acagtaaaga tggcgaccag
      241 agttatatcc gttttggttt taaaggcgaa acacaaatta acgatcaact gactggctat
      301 ggccgttggg aagcggagtt tgccggaaat aaagcggaga gtgatactgc acagcaaaaa
      361 acgcgtctcg cttttgccgg attgaagtat aaagatttgg gttctttcga ctatggccgt
      421 aacctgggcg cgttgtatga cgtggaagcc tggaccgata tgttcccgga atttggtggc
      481 gactcctcgg cgcagaccga caactttatg accaaacgcg ccagcggtct ggcgacgtat
      541 cggaacaccg acttcttcgg cgttatcgat ggcctgaact taaccctgca atatcaaggg
      601 aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg gcttcggcac gtcattgaca
      661 tatgactttg gcggcagcga tttcgccatt agtggtgcct ataccaactc agatcgcacc
      721 aacgagcaga acctgcaaag ccgtggcact ggcaagcgtg cagaagcatg ggcaacaggt
      781 ctgaaatacg atgccaataa tatttatctg gcaacttttt attctgaaac acgcaaaatg
      841 acgccaataa ctggcggctt tgccaataag acacagaact ttgaagcggt cgctcaatac
      901 cagtttgact ttggtctgcg tccatcgctg ggttatgtct tatcgaaagg gaaagatatt
      961 gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg tcggggctac atattatttc
     1021 aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc aactggatag cgataacaaa
     1081 ttgaatatta ataatgatga tattgtcgcg gttggcatga cctatcagtt ttaatgatta
     1141 ttgccggatg tgatgcatcc ggcacatttc actcacgccg ttaacttcac cggctcgtca
     1201 cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc
     1261 tcgtgaaact cacgcagggt gtgctccagt cgttcaacca cttcagtgtc ttttaccgga
     1321 acgctcttcc agtcgcctgc tttatcgaac agaccaaact ggtagctgta ggtaaaacgg
     1381 gattcctgcg cttccagctc catccaccag ccccagaatt cacgcacttc cggtgccggt
     1441 ttcacgttga cgcatacagc cagacaatcg aaaaagaatc gattatcttc gcacttacct
     1501 tcacgaatat acgggcctag tgcggtaaat tttttgatca atctgctctt cgggtgtcca
     1561 ctcggtaacg tcattgctat ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat
     1621 ttagcaaatt tttaacacaa gcgtttttcg atccagccgg tgatttcctg aagacctttg
     1681 tcaaaattcc gatacaccgg gttaaatggg atctctaata atttaccgtc agcagatgat
     1741 gaggtgatta agcgtgagtc ctcttccggg ctgaacggat cgttcttcca gtagcctgat
     1801 aacattggcg ttgggcagcg acgtcccagc aatccttgca cttttaatga atagcgattc
     1861 agctccacgc gcaacgcgtc atcggaagca tcatgcatcc ccaaacgact cgccagaacg
     1921 tcaagataca tttccggcac ctgttgctgg cacttaaaat cactcaacag ggtatgaact
     1981 accggaccaa gacaggcaac cgctttcaga cgcggcgatt caaggtatgc cagacgcacg
     2041 gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt
     2101 acgttaggca gcgcctttaa gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc
     2161 cattttgaag aaaagcccac cgacggcata tcaatagtca gcatcgcaat gccgcgcggc
     2221 gcaaaataac gttcatacag gctgtaatag tccgtctgca tcgcatccag accaccacac
     2281 attaatactg tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc
     2341 gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc
     2401 tcttcatagg cgcggtttga taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga
     2461 tgaggatagg cggcaatgtt gtacaacgta gccgcatgca gccagtgtcg accactgagt
     2521 tgcggatctt cttccgcaca ggctttttgt tgccacacca tcgcctgggt ggcccactca
     2581 taaatccagt tgccaccacg gtagccaatc acagtatcgt ataaatcatc gtcggtacgt
     2641 tcagcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcacgtgg gtcaataccg
     2701 cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca
     2761 agagccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt
     2821 tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat
     2881 gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac
     2941 gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac
     3001 gcccatatcc cacggctgtt cgatccaggt atcttgcggg atatcaacaa cataatcatc
     3061 aaccagcggg cgaccggccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat
     3121 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc
     3181 ttcgccatcg ccttctgcac gtttcagcac tttaagctcg cgctggttgt cgtgatcgta
     3241 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc
     3301 cggtaccaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
     3361 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
     3421 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
     3481 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
     3541 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
     3601 gcaagcctgt cttgtgttga caacattttc tgctaaccct gtgacctgca atactgtttt
     3661 gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc tcaattatct ccacagccgc
     3721 tgtgggatat ttttgccaaa atctgttcta ttcctcaccc gtcctatcat gaagagcaac
     3781 tcgctgaata cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg
     3841 taggtaatat cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg
     3901 tcttacaggc ccacctcgat atggtgccgc agaaaaataa cgacaccgtg catgacttca
     3961 cgaaagatcc tatccagcct tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc
     4021 tgggtgcgga taacggcatt ggtatggcct ctgcactggc agttctggct gacgaaaacg
     4081 tggttcacgg cccgctggaa gtgctgctga ccatgaccga agaagccggt atggacggtg
     4141 cgttcggctt acagagcaac tggttgcagg ctgatattct gattaacacc gactccgaag
     4201 aagaaggtga aatctacatg ggttgtgcgg ggggtatcga cttcacctcc aacctgcatt
     4261 tagatcgtga agcggttcca gctggttttg aaaccttcaa gttaacctta aaaggtctga
     4321 aagggggtca ctccggcggg gaaatccacg ttggcctggg taatgccaac aaactgctgg
     4381 tgcgcttcct ggcgggtcat gcagaagaac tggatctgcg ccttatcgat ttcaacggcg
     4441 gcacactgcg taacgccatc ccgcgtgaag cctttgcgac cattgctgtc gcagctgata
     4501 aagtcgacgt cctgaaatct ctgatgaata cctatcagga gatcctgaaa aacgagctgg
     4561 cagagaaaga gaaaaatctg gccttgttgc tggactctgt agcgaacgat aaagctgccc
     4621 tgattgcgaa atctcgcgat acctttattc gtctgctgaa cgccaccccg aacggtgtga
     4681 tccgcaactc agacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga
     4741 ccatgactga taataacgta gaaattcact gtctgatccg ttcactgatc gacagcggta
     4801 aagactacgt cgtgagcatg ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag
     4861 cgaaaggcgc atatcctggc tggcagccgg acgctaattc tccggtgatg catctggtac
     4921 gtgaaaccta tcagcgtctg ttcaacaaaa caccgaacat ccagattatc cacgcgggcc
     4981 tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga catggtttct atcgggccaa
     5041 ctatcaccgg tccacactct ccggatgagc aagttcacat cgaaagcgta ggtcattact
     5101 ggacgctgct gactgaactg ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc
     5161 cggatggcgt ttaatcgcct tccggcagtt tcatccttca ttatccttcg ataaaagcca
     5221 tccctgtaaa tgtccgtctc gggttgccac gttcaatctg gtgatggaac attcgccgct
     5281 gcgatttcag caccgcgcta ttttcctgtt gctgctgctc cagcttccag gcaatcagca
     5341 atcgtgccag ccgcttgtta gcatgctgac tacgctctga ctgaaccttc acgctaatac
     5401 cggatgccaa atgcgtggcg cgtaccgccg agtcggtttt attgacatgt tgaccgcccg
     5461 gccccgacga acgcaacgtc tcataacgga ttgcatccga ttgttcctgc tcatcagcgg
     5521 taaaacgccc aatgcccaga aaccagtttt tgcgcccatg atgaggccga tacggactcg
     5581 gacaaatcca ctgaatagcg ccacaccagc tttcgcttaa tgcccatgcg ttatcgccat
     5641 ccagagaaat cagcgccgaa cgcagcgtgt cagagtagcg gcccgtttct gtttccaaca
     5701 ccgttaccgc gacgtcttgt cgggcagctt ctttaataag cctgtccagc gcctttttca
     5761 ctgcgagaca acattcttcc ggcccctgcg ctgaggagag ttgtagcaag atcatccttt
     5821 tttccctcca ctgtttttga gcgttagcac cggacgcagc cgcgcgacag gaataattaa
     5881 accggcctgc accagacatt gcaccacgct ttcagccgat ttataggctt gtggcgcttc
     5941 ttcaaagatg agttgtttat cgcgacaaat tacccgactg ccaagttcag tacgcgagag
     6001 ttgcgtcgcc gtgtatttcg ctgccagacg ccctttacac tcggtgcgcc cccatttacg
     6061 ccctgcccca tgcgccagcg agtggagcgt tttttcgttc gcaacaggct gtacaatcca
     6121 ggagtaatca ccacgtgaac cgggaataat caccagaccg ttgtcatccg gcgtggcacc
     6181 tttacgatgc aaccagccct gttgatcgcc gatttgacac gcgctaacaa agttatgcgc
     6241 cacatccaga accggacttc ccgtggcctt aacttgttgc attatgcgca aagcgatcat
     6301 ctggcgatta atacacgcaa acgccagcgc atcgtcatgt tccgcaatat accgtagcgc
     6361 gtcgtcactg ccttgaggca aaccatgatg cgagaacgag gcaatatgcc gctgtaaaat
     6421 agactgcccc accccccgcg agccgctatg aaccaacagt tgcagatgtt gtgcatccag
     6481 acctgccagt gcaaacagtt cagcgttgat gatttgatca acttgttgca gttcagcgaa
     6541 atgattgccg ccaccgatag agccaagcga gctgcgccac ggatgctgtg caaatgctga
     6601 cggcaggttt tcctccagcc agctttcttc agcaacgtca tccagatctg ataatcgctt
     6661 ttcaaactta tcggcgttgt atttgcgagc gagaatatct gtttgccata gcgccatacc
     6721 gcagccgata tcattgccga ccagtgccgg ataaaaacga ccaacagaga agaacgctgc
     6781 gccaatcgga tagccgcgtc cgggatgtaa atctggcatc cccactacgc gctgcatgtt
     6841 gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa ctctcgatcc acaggtcatc
     6901 agatgcgatg gtaaataccg catcggataa gggacgaata taattgccca tataccattc
     6961 cgtaaatgga ctcgtcatac ttcaagttgc atgtgttgcg acttgaatta ttgagagtaa
     7021 caattaaaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga attaatcctg
     7081 gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc ttgcagtttg
     7141 tggaataaga aaacgcgcac atactaggct tgcgttattt tcattgcaag ctggatttaa
     7201 tgttgctgtt tataacgcat ataaaaatta ataaaccact ctccccgaca ttcaacgcgt
     7261 tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt tatgcttgcg
     7321 tccaccgtaa gttcggattc agacttaatc aaaggtttta acaaagcgct ggcaacccca
     7381 cggcgggtgt attcagggtc aacaaataac atatcgatat agtgttcaac gcaggtaata
     7441 aagccgacca gttttgcatt aatgactgca acccgcactt gtgatttcgc gagtttctcc
     7501 ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gttgtggtga ataatgctga
     7561 ctggcagtca tcgtaaccgc tctaatgaaa atagcgcata gttgctgaaa atcgccaggc
     7621 tgatagtctc ttatttgtat gttattcata atcccagcac cagttgtctt tccatttgcg
     7681 ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg cggcgttcat
     7741 cccaggtttt acgcgcggtg gcgattaagt cagctttatt cagccgcggc cagacgtgct
     7801 cctgagtggt ttgctgaaaa tcgtcgaact ttaatttcac cccctggcgg gcaatcagta
     7861 aatcaggttt tacctttgcc agacggcgtt caagttccgg atacagccgc tcgataatcg
     7921 cttcacattc agaccagtgg tggatatctt ccgccatcgt gcgttccacg ccgacggatt
     7981 ttcgcaaccg ttcgctgtta acgtcgcgct cgtcaatccc ctgactacgc tcccacaaaa
     8041 tgcggccaaa tttgccaaag cgtttaagca gcatcaccag atcacacttt tgtacatcac
     8101 cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggga
     8161 tttttgccag cggtaaggtt tgtaaaaatg ccggaacttc tgccggcgta atcacaaact
     8221 gaccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact ggtgccacgc
     8281 ccgcagacgc tgtcagttgc agctcgctga aaatcgtctg gcggatttcc tgggcgatga
     8341 gggtcgcaga accgtggcaa tggacgctat cggtgacgtc gagataagcc tcatccagtg
     8401 acaacggttc aatacgcgag gtgtagcgcg agaagatttc acggatatga tttgaggctt
     8461 ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg cgggcataat ttgagcgcca
     8521 tccctgtcgg catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga
     8581 tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat
     8641 tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc cacatgaatg attttacgca
     8701 ttactcacct ctcaacactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa
     8761 agattcagca tttaacgcgt tcgctgcgaa agcacctgct gcttatccag cttttgcacc
     8821 agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca
     8881 cttttggtca gcaccataat ctcaatgcgc cggttgcccg cgcttttcgg atttttggaa
     8941 tccagcagca tctggtccgc cattgcgctt acctgcatca ctttatcttc cggcattccg
     9001 gcctcttcca gcacccgacg agccgaaagc gcgcggtcac ccgaaaggtt ccagttgttg
     9061 tagatattgt ttttgtaagc catcgcatcg gtatgcccgg taataataat tttgttatcg
     9121 agcgagtcga aaactggcgc gagctccacc agcagcgttt taaagaacgg cataatctgc
     9181 gcgctgccgc gttcgaacat attgcgattc tggtcgtctt taatcagcac gcgtaatccc
     9241 tggggaacaa tctccatctc cagattcgct tccatatgcg catcacgggc aatggtggtg
     9301 atactggttg ccagctcgcc aagctcggtg gcggatttct tcttcagcag cgccgttttc
     9361 tcattgacgt cgcgcgcttt tttctccgtt tcttccggca ccgccacggt ggcaggtttt
     9421 ggcggatgag acgggctgat tttatttaac ggcgacagcc caccaccgtt aaaaatcgac
     9481 tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc
     9541 aactttattg tcgtcaccaa aggggccgag cgtatctccg aggtttctgc ccgctttacc
     9601 ctggacgcga tgcccggtaa gcagatggcg attgacgccg atcttaatgc cgggttgatc
     9661 aatcaggcgc aggcacaaac ccgacgtaaa gatgttgcca gcgaggccga tttctacggc
     9721 gcgatggacg gggcatcgaa atttgtgcgc ggggatgcta tcgccggtat gatgattctg
     9781 gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca aatacaacct gagcgccgac
     9841 gccgccttcc agcagtatgt gctgatgacc atcggcgacg ggctggtggc gcaaatccct
     9901 tccctgctac tctccaccgc ggcggcgatt atcgtcaccc gcgtcagcga caacggcgat
     9961 atcgcccatg acgtgcgtaa ccagttgctg gcaagcccat cggtgctcta caccgctacc
    10021 ggaattatgt tcgtactggc ggtagtgccg ggaatgccac atctgccgtt tttgctgttc
    10081 agcgccctgc ttggctttac cggctggcgg atgagcaaac gcccgcaggc ggcggaagcg
    10141 gaagagaaaa gtctcgaaac gctgacccgc accatcaccg aaaccagcga gcaacaggtc
    10201 agttgggaaa ccattccgct gatcgagccc atcagcttaa gcctcggcta caagctggtg
    10261 gcgctggtgg acaaagccca gggcaacccg ctcacccagc gtattcgcgg cgtgcgacag
    10321 gtgatatccg acggcaacgg cgtgctgctg ccggagatcc gcattcggga aaacttccgc
    10381 cttaagccca gccagtacgc tattttcatc aacggcatta aggctgatga agcggatatt
    10441 ccggcggata aactgatggc gctgccctcc agcgaaacct acggcgagat agacggcgtg
    10501 ctggggaacg acccggcgta cgggatgccg gtgacctgga tccagcctgc gcagaaagcg
    10561 aaggcgctga atatggggta tcaggtgatc gacagcgcca gcgtgattgc tacccatgta
    10621 aataagattg tgcgcagcta tattcctgat ttatttaact atgacgatat tacgcagttg
    10681 cataaccgtt tggcgtcgat ggcaccgcgc ctggcggagg atttaagcgc ggtgctcaat
    10741 tacagccagt tgctgaaagt gtaccgggcg ctgctgaccg aaggcgtttc cctgcgcgat
    10801 atcgtcacca tcgccaccgt gctggtcgcc agtagcgcgg tgcccaaaga tcatattctg
    10861 ctggcagccg atgtgcgcct ggcgctgcgg cgcagcatta cccatccgtt cgttcgcaag
    10921 caggagctga cggtgtatac cctgaataat gagctggaaa atctgctgac taacctagtg
    10981 aatcaggcgc aacagggcgg taaagtgatg ctcgacagcg tgccagtgga cccgaatatg
    11041 ctcaaccagt tccagagcac gatgccacag gtgaaagagc agatgaaagc ggcggggaaa
    11101 gacccggtgc tgctggtgcc accgcagctg cgccctttgc tggcgcgtta tgcaaggttg
    11161 tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc cggatgagct ggagttgaag
    11221 attatggggg cgttgagttg atgggggatt cttttccatg ctttgcagca cctgacacca
    11281 ttaattgacg tcacactgca aagagactac gatgacacca tacaatgacg tcatggattg
    11341 atagtatgat taaggtcagc gcgctaggtt ctcgtcatag aaaaacattc gacgatgtaa
    11401 tgacggagcc gccaaaatct ggaattaaat gggatgatgt tgttgcactc attaaagctg
    11461 tgggtggaac aataaaaaat aacaacggct ccagacgaaa atttcagatt ggaacgacca
    11521 aattcagcac acatgagcca catccaaaga atgtgatgga taaaggcgca gtagcaggtc
    11581 ttaaggagtg gtttgttaac tgtgtagggg tagattatga ataaagcatc gaacattatc
    11641 acaattaaag gtcaaccagc cgcagtgaca tttgaagccg atatcaacgc atttcgcggc
    11701 aagttcctca atgtaaacgg ttattgtgac tttgtagcca caagtattga ggcattatat
    11761 cgtgaaggcg aaagcgcgct ggatgagtgg atggcggatt gtgaagagga cggtattgcc
    11821 ccttttcgtg aagaagaaga gcaaaaacgc ctgacgcttc gtgttcctca tcacattgat
    11881 tcgcggctga ccatcgtcgc cagacaacat tccatttcaa aaaaccagct cattgttgat
    11941 gtcttagaac gtgagttttc agcccagatg taaacaggtc ggatgccgcg tggcatccga
    12001 ccttttgcca gttcgtcaat attacccatt tacaaaatcg tctcttccgt caatatccgc
    12061 cgcgcgccta aaaagtgatc ctgccagaaa ggttcagcta accgacttac ccgaatgatt
    12121 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
    12181 gcaatctcgc ggctgtggat atggaaaaat agcaaatctc cccggcgcag gtcgttattc
    12241 gccacaatgg ttgcccggcg atagtgatac atctcattag ccgtgcgtgg gagtttcgcc
    12301 tcaaggatct tgttgtaggc ataaaaaact aacccgctac agtcgaagcc ttgatcgggc
    12361 cgcgtaccgc cccagacgta aggcttgcca agctgttgct ccagacggtg aatcgccact
    12421 tcggtgatgt tatgcagatg gtcgctgctt aagaaatggt tgttttccgc cagcgcctgc
    12481 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgt
    12541 tcgcggttgt gctggcgtag cgcccttctg ctttcggcat tgccttccac aatatagctg
    12601 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacgatt ttgcatccgc
    12661 tggcgggcgg cgtagctgaa agtgatatgt gatgtggtgg cgctggcaaa tgaagaaaac
    12721 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaggacata
    12781 ctcaataatg gctgatcatg cattgatgtt gcagtgtatc ccggcccgta cctgaacaaa
    12841 cttaataaac cattattata aaattaataa atatatttca gttaattatg taaagacatc
    12901 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
    12961 ataaatatac agcacaggag ataccccaat ggctgctaac gcatttgttc gcgcccgaat
    13021 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
    13081 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
    13141 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
    13201 tcataaggcc aaagacgccg atgatttatt tgataaatta ggaatttaaa tatgattcaa
    13261 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
    13321 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
    13381 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
    13441 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
    13501 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
    13561 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
    13621 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
    13681 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
    13741 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
    13801 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
    13861 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
    13921 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
    13981 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
    14041 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
    14101 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
    14161 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
    14221 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcagctga
    14281 agccgggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
    14341 gccgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
    14401 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
    14461 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
    14521 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
    14581 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
    14641 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
    14701 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
    14761 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
    14821 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcgcgg
    14881 gtaaatgggt gagtattttc cagcgccacc tcgccccgat tagcctggcg aatatgagcc
    14941 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
    15001 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
    15061 ccatctttat gtggcccggt tccaccacca cgctgtacaa gtccggtgaa actaaagcag
    15121 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
    15181 agcgggagcg aatggccccc ggcgaagatt gatttaagaa ttttcaggtc ggatgcggcg
    15241 taaacgaatt attcggccta cggttcgggt acagatttgt aggcatgata agacgcgtca
    15301 gcatcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccagcct
    15361 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
    15421 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggtacgcgaa tttcgatatc
    15481 cgccgtgcca gccattttgc cgccgtcttt accggtcagg gtgatcactt tcatcccctt
    15541 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
    15601 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
    15661 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
    15721 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
    15781 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
    15841 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
    15901 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
    15961 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
    16021 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
    16081 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
    16141 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
    16201 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
    16261 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcgg
    16321 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
    16381 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
    16441 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
    16501 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
    16561 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatca
    16621 cccatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgtttct
    16681 tcgcgatgat catcaaaaaa gagtacggcg ggctggagtt ctcggcttat gcccagtctc
    16741 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccaa
    16801 actcattagg cccgggcgaa ctgttgcaac attacggcac tgacgagcag aaagatcact
    16861 atctgccgcg tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc agcccggaag
    16921 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
    16981 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga
    17041 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc ggaaaagctg ctgggtggcg
    17101 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
    17161 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
    17221 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
    17281 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
    17341 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
    17401 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
    17461 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
    17521 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
    17581 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcagagcaac ttcctggcgc
    17641 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggccaacatt ctgacccgca
    17701 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
    17761 aagcagcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
    17821 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
    17881 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
    17941 acctcgccct gctttctgat gtctcgatgg ctgtgctggg cggcagcctg aaacgtcgtg
    18001 agcgcatctc ggcacgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc
    18061 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
    18121 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
    18181 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
    18241 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
    18301 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
    18361 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
    18421 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
    18481 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
    18541 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
    18601 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
    18661 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
    18721 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
    18781 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
    18841 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
    18901 ctcatcacct gatacgtctc atctcccaac gttacggttt gtgcgggagt ataagtaccg
    18961 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
    19021 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
    19081 gcgatgacca gagcggcgac tgtcgttatt gccttaaaca tcattcctcc cgagcttatc
    19141 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
    19201 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
    19261 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
    19321 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
    19381 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg
    19441 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg aactggatga
    19501 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
    19561 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
    19621 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
    19681 tgattgtgga atggcgcggc tggcgtattt tgccgctggt gtgctacgac ttacgttttc
    19741 ctgtgtggtc gcgcaatctc aacgattatg acctcgccct gtacgtcgcc aactggcctg
    19801 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
    19861 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
    19921 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
    19981 gcattgatgc cgagctgtcg atggcggtgt taagggaata tcgcgataag tttccggcat
    20041 ggcgggatgc ggatgagttt aggttgtagt gagtgggggc tgtatttagg gcggggaatt
    20101 cagaaaggga tgtttagtgt gctgagcgag agttaaataa taaaaaactg ggaagattta
    20161 ataatccccc cgcagggcaa gagcatgaat taaatttgtg ttaaattttt cattttgtca
    20221 gttcgaaacc caagctctga gcaggatgca agcacttctt ctatataaca tgtgtttaac
    20281 gccagggcaa gattacgaaa gcccgctccc cgcaaggact gacgccagat agtttctgtc
    20341 catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa
    20401 aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc ctcttcttat
    20461 tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccaagtg
    20521 ccagtgattt cggatcgctg tggcgaattt ctcagcggtt aaatcagcag aactgatata
    20581 atatctgacc gtcatttctg gctctttct
//
LOCUS       DAZGCS010000084        20569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGCS010000084 DAZGCS010000000
VERSION     DAZGCS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.084"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHK01_003410"
     CDS             complement(<1..209)
                     /locus_tag="ACHK01_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8098316.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK01_003411"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK01_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO8098317.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK01_003412"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK01_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO8098318.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK01_003413"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK01_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO8098319.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK01_003414"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK01_003414"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO8098320.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK01_003415"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK01_003415"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO8098321.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK01_003416"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK01_003416"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO8098322.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK01_003417"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK01_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8098323.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK01_003418"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK01_003418"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8098324.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK01_003419"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK01_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO8098325.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK01_003420"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK01_003420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8098326.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK01_003421"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK01_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO8098327.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK01_003422"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK01_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO8098328.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK01_003423"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK01_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO8098329.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK01_003424"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK01_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8098330.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK01_003425"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK01_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8098331.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK01_003426"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK01_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO8098332.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK01_003427"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK01_003427"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO8098333.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK01_003428"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK01_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO8098334.1"
                     /translation="MQARVKWVEGLIFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHK01_003429"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHK01_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO8098335.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK01_003430"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK01_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO8098336.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALQPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK01_003431"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK01_003431"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO8098337.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK01_003432"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK01_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO8098338.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK01_003433"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK01_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO8098339.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK01_003434"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK01_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO8098340.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         5060 a         5624 c         5089 g         4796 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccacca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaaattaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg caggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gcaacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagc
//
LOCUS       DAZGCS010000085        20278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGCS010000085 DAZGCS010000000
VERSION     DAZGCS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.085"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHK01_003435"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHK01_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO8098341.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHK01_003436"
     CDS             complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHK01_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8098342.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="ACHK01_003437"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="ACHK01_003437"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO8098343.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHK01_003438"
     CDS             complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHK01_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO8098344.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3608..4096
                     /gene="yecT"
                     /locus_tag="ACHK01_003439"
     CDS             3608..4096
                     /gene="yecT"
                     /locus_tag="ACHK01_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO8098345.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="ACHK01_003440"
     CDS             complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="ACHK01_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO8098346.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="ACHK01_003441"
     CDS             complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="ACHK01_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO8098347.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLESLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="ACHK01_003442"
     CDS             complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="ACHK01_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO8098348.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="ACHK01_003443"
     CDS             complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="ACHK01_003443"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO8098349.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="ACHK01_003444"
     CDS             complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="ACHK01_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO8098350.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="ACHK01_003445"
     CDS             complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="ACHK01_003445"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO8098351.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="ACHK01_003446"
     CDS             complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="ACHK01_003446"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO8098352.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11019..12620)
                     /gene="tap"
                     /locus_tag="ACHK01_003447"
     CDS             complement(11019..12620)
                     /gene="tap"
                     /locus_tag="ACHK01_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO8098353.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12666..14327)
                     /gene="tar"
                     /locus_tag="ACHK01_003448"
     CDS             complement(12666..14327)
                     /gene="tar"
                     /locus_tag="ACHK01_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO8098354.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSTFRLAASPLTNKPQTPSRPASEQPPTQPRLRIAEQDPNWETF"
     gene            complement(14472..14975)
                     /gene="cheW"
                     /locus_tag="ACHK01_003449"
     CDS             complement(14472..14975)
                     /gene="cheW"
                     /locus_tag="ACHK01_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO8098355.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14996..16960)
                     /gene="cheA"
                     /locus_tag="ACHK01_003450"
     CDS             complement(14996..16960)
                     /gene="cheA"
                     /locus_tag="ACHK01_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO8098356.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16965..17891)
                     /gene="motB"
                     /locus_tag="ACHK01_003451"
     CDS             complement(16965..17891)
                     /gene="motB"
                     /locus_tag="ACHK01_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO8098357.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17888..18775)
                     /gene="motA"
                     /locus_tag="ACHK01_003452"
     CDS             complement(17888..18775)
                     /gene="motA"
                     /locus_tag="ACHK01_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO8098358.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18902..19480)
                     /gene="flhC"
                     /locus_tag="ACHK01_003453"
     CDS             complement(18902..19480)
                     /gene="flhC"
                     /locus_tag="ACHK01_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO8098359.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19483..19833)
                     /gene="flhD"
                     /locus_tag="ACHK01_003454"
     CDS             complement(19483..19833)
                     /gene="flhD"
                     /locus_tag="ACHK01_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO8098360.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4863 a         5570 c         4938 g         4907 t
ORIGIN      
        1 ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
       61 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata ctcgtccgcg
      241 tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac ccgggcaatg
      481 cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc gaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggacc
      661 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtacc
      781 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
      841 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     1021 atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc
     1081 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca gaacgatttc
     1141 aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta gctccacaat
     1201 agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     1321 ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     1501 agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaagatgaag agttcgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc cgtgatgacg
     2461 tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat ctcaaagcca
     2521 aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca
     2581 aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgtgcg aaatatcgtt atgaatcact gcatgccgat cgcgtgctgt
     2701 attacatcga ctcccgtcag catcaacacc taatgcaggc atgggcgatc gtccgtaaag
     2761 caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggta
     2821 aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg
     2881 atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat atgccagctg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc cgtaaggcag
     3121 aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggctgg tctgttctct ggcttctacg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt gagacagtag
     3421 agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct gatcaggctt
     3481 ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct gactgttgga
     3541 taagaggcca gaagcgtaat atccggcccc agggaaacga taacggttga atttaaggaa
     3601 taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc aggcttacgc
     3661 agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt gttggcaaaa
     3721 aaattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac aagaatacaa
     3781 tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata tagaacaagt
     3841 gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca
     3901 gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa atgaggacgt
     3961 tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga tcatacagac
     4021 caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt atccaagccc
     4081 tttgacaaga ggataattca catctttttg gcatgttttg ttgcaagcta ttcctgataa
     4141 ataattgcaa caagacatcg agcctttttc actgagttat taaacatact cgcgagcgcg
     4201 taattttttt gtccttcagc gataattcac aatcacttca ttacgttgta cctttagcgg
     4261 tggaattaac cgcccaccgc ctggcacttc ccagataaat cgcaacggtt ctgctgccgc
     4321 tataccggaa aaggccacgg tggttccgct ctgcccctct aattcgacac aacgagattg
     4381 cgaacacaag cgaacccgca gtcctgaagg tgtcgggccg ataagctgat aacgccacgc
     4441 taccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgcag acgacatcga
     4501 ctcaccgcga tgatttagcg taattcctac actacttgcc tgccacatcc cctccccggc
     4561 ggcttgcacc agcagcggaa acaataatat tgctaataag gttctcatta tttgccgcca
     4621 attgtcgccg tcatgcggat atgtcggtta tcagacagtt ccagattcga caggaccact
     4681 aactgcggca agctgcggcg caggaagcga gacaataatg gtcgcagcgc gtggttcacc
     4741 aacaatactg gcggcgcacc cagcatctcc tgacgggata gcgcttcctg agtttgcgcc
     4801 agtaaacgat ccgccagccc tggctccagt cctcccccgc cctgcagcgc ctgtagtaac
     4861 aaactttcca gcggtgtatc gaggccaata acatggactt catctttgcc aggaaaccac
     4921 tgctgggtaa tcgcccgtcc caacgccacg cgcacgacgg cggttaattc atgtggatcg
     4981 ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt
     5041 actttttcat cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca
     5101 ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccaa cagctgttgc
     5161 gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt
     5221 gctaccaccg tgctggcctc aaccactgtg tacccctgaa tctgcgcctg ttcttttagc
     5281 gcactttcaa tccagatagc attcaggcca aatgccggat cgacggtcgc ctcaccaggt
     5341 aacgtcccgg cagcggttcc agggttaatc gccagccagc gccccggata agcatcacca
     5401 ctgccaatct ccacgccttt catcaaaatg cgatagcggg caggttgcag atccatattg
     5461 tcgcgaatgt gcaccactgg cggcagaaat cccatctcct gggcaaattt cttgcggata
     5521 ctgcgtatac ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga
     5581 taacccactt ccattcccag agaatcttcc agttgtacat cgttccacgt cgcttcgaca
     5641 acggtattat tctctgccat ttttaccggt ttgggttcga caggcgcttt ttgttcgcgt
     5701 ccgcgtatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa taccaggttc
     5761 ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa cataacgctt
     5821 gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc
     5881 gtaacgatga cccccgcggc ggtagaaatc accagcgccg gaatttgtgc caccagaccg
     5941 tcgccaatgg tcaatagtgt ataactttcc gccgcgtgtc ccatgctcat gccatgttgc
     6001 agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcgatg
     6061 gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga
     6121 gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg
     6181 gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct
     6241 gcgatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac
     6301 actacgatac cgatagcgaa attgccacca acgaggaagt gaccgaatgc ttcgaccacc
     6361 ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg
     6421 ttaagtgcca gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc agcaaactca
     6481 agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag
     6541 gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac
     6601 aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg
     6661 cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc
     6721 cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac gccagcgttt
     6781 cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg
     6841 ttgaccaatc tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg
     6901 gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc
     6961 gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt
     7021 cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcagc
     7081 agctcgctgc atctgacgga tccgcccttt aacatgaggg tcaccttcgc tttgtttgaa
     7141 ctcatcacga atatcctgcc gtgacatacg cagctttttc aggtggctga atatttggaa
     7201 aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagtg cgcatagccc
     7261 taccaaatcc atcgcattac ccatggcggt aatcggagac tcggccatca agcgcatcat
     7321 ctgcggccag tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa
     7381 aattgcttta agcaactccg cgccagtctg agccgagaac atccgtttaa tgcccggtag
     7441 cgggttgagt ttggaaaact tcggctgcaa ggatttgccg ctaaatacca gccctcccag
     7501 catgaccgga gaaatgagcg ccaccagcac cacgccgcta atcagcggca gcagcgccag
     7561 catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat
     7621 actgtgatca aaatgcagcc cagcggagag catgcccgac aatcgacggg ccagcgacac
     7681 accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgagg tcagttcacg
     7741 ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcttc
     7801 tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcgtgaa
     7861 tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag
     7921 ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt
     7981 atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag
     8041 actatccaac aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc
     8101 gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg
     8161 gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat
     8221 ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag
     8281 ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt
     8341 taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg
     8401 ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact
     8461 gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg
     8521 cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa
     8581 actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg
     8641 ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct
     8701 caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc
     8761 cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca
     8821 ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc
     8881 catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact
     8941 tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttaa
     9001 gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt
     9061 ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt
     9121 tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt
     9181 gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca
     9241 tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag
     9301 gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac
     9361 cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc
     9421 actacgcgac agctccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac
     9481 acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc
     9541 aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag
     9601 cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc
     9661 aatcagtttt tcagaactca acaacggacc cgccttcagc gttgtcggtg ctgacaatgg
     9721 cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct
     9781 atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc
     9841 cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac
     9901 aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg
     9961 catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcgac
    10021 cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt
    10081 catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt
    10141 taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg
    10201 ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag
    10261 cggcgcaaaa tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag
    10321 atcgcatcaa acggccccgg cacggtgtac tgtttctcca gtagattcag cggggcaaaa
    10381 tcaacatagt tcgccaactc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct
    10441 cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa
    10501 ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag
    10561 cgtccgggcg cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg
    10621 ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc
    10681 gcgagcagag ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa
    10741 ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga
    10801 ccgaaatccg ttagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg
    10861 cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga
    10921 aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca
    10981 cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt
    11041 tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca
    11101 cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct
    11161 accagcgagg cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc
    11221 tcaatccccc gctgttgttc ttccgacgca gaggcaattt ctcccataat gtcgttcacg
    11281 cgggtcaccg aactgacaat atcgatcatg gtcgcggcgg cgttattcac cagtttcgaa
    11341 ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc
    11401 gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc
    11461 tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca
    11521 attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc
    11581 atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt
    11641 tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat
    11701 tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag
    11761 agatcgttat tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta
    11821 cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg
    11881 gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg
    11941 ctgtcaaaat ggctaccaat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac
    12001 agcgcactgc tgatgtagat tgccgcaaca ataatcatgc tgataaacac cagcgccgaa
    12061 atctgatagt tacgctgact ttgcgcactg gcggcttcca gcacatggtt gatctccagc
    12121 tgccaggcct caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg
    12181 aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag
    12241 cgggcaaaac ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc
    12301 atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc
    12361 atcaacgttt taatgtcatc cgccggatag ctaagtgccg tcagagtgcc cgctttgttc
    12421 agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga
    12481 ttgctctgct cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg
    12541 ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa
    12601 attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaat
    12661 actcgtcaaa atgtttccca gtttggatct tgttcagcaa ttcgcagtcg tggctgtgtc
    12721 ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct
    12781 gccagacgga acgtggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagcg
    12841 gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc
    12901 gaaaccgcca atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc
    12961 tcgcccataa tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg
    13021 gcgctttcga ccagcaccga accggtatca acgcgtgaga cggagtcttc aatgagggct
    13081 ttgatctctt ttgccgcctg ggcgctacga ctggcaagat tacgcacttc acccgccacc
    13141 acggcaaaac cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg
    13201 aggatattag tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc
    13261 gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc
    13321 tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt
    13381 tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc
    13441 tgctgttcag tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca
    13501 tagatggcat ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata
    13561 tgtgaaacgc tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc
    13621 gccaggttac caccggcgat ttcgcgaatg tgagcaataa tttttgccag cggagtaagc
    13681 aacatacggc gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata
    13741 accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga
    13801 tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt
    13861 ccctgggttg gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt
    13921 tcagttaacg ctgtgtgata gtttttatat ttttcatcaa tattacgact ggtagcgacc
    13981 atttcaggta acggtgccat gcttttgaat tttttataat gagtcgcggc ctgggccaat
    14041 gttttcctgg cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc
    14101 atcatcatcc gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag
    14161 gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg
    14221 ctatggtgaa gggaagaaaa aaacagactg ccggaaataa gctgtaacag tgcgaatacc
    14281 cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct
    14341 gaaaacaagt taatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc
    14401 gccgcgaaat tgaaataaaa aacccgatgc gcagatcatc ggggtcattt caattgagga
    14461 aatcgggaga attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg
    14521 ttcagcagtt tttcgatgtt caccagaatc aacatccggt cgcccagtgc gcccagtcca
    14581 gtgagatatt ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc
    14641 gtcaatgaaa gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga
    14701 ttcaggacga taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt
    14761 cgtaagtcaa caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct
    14821 ggcgtgttcg caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata
    14881 tcaataccgt actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg
    14941 ctggccagct ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg
    15001 cggcggtgtt cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa
    15061 tcagtgccac gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat
    15121 agttactttc aaggttttta accacaacct ggtgctgacc aattaattga tccaccagca
    15181 aggcgtagcg gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt
    15241 tcgcgcccgc gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca
    15301 cttccagcac ccgctcgccg ccggccagtg gatgcagatc ggcttcacgg ggttgcagtg
    15361 attccataac agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc
    15421 cgtcgaggat ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct
    15481 tcgactggat ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc
    15541 cgacgccgcg cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata
    15601 tcagcatcgc gacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg
    15661 cttttgccag aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa
    15721 tgttgccgcc ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg
    15781 cgagccgttt ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca
    15841 gcgggtcgat aatgcgttct atcaggcttt tgtcgagttc agtagaactg cccaccagcg
    15901 tcagttctac ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac
    15961 taaaaacata ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg
    16021 cgttacgttg taactgcccc atgctggtta tcaaatcacc atgattaacc gggtccagtt
    16081 cgctggaacg ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta
    16141 attgatcaac cttttctacc gctacacgga tgctggtgga ttcattgctg cgcgtcgttt
    16201 tttcccgctc cacgcggccg gttggcgctt gttcggctgc cagtttaagc actggtgggg
    16261 tggatatttt tggcgagact tctactgttt caaaggtaat ctgatcggct tcaatcacaa
    16321 aacagagtac cgctgtgatg tcatcttcgg cgatgtcgcc cggtaatatt gccgagagcg
    16381 aatccgcccc tttcaccacg tcagttaacg ttgtcagatg tcccagttct tcttccagca
    16441 ggtcgacttc cccggccttc aggcgcgaaa ggataattcg tcgcggcgac tgactgcgac
    16501 tctgctcatc ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg
    16561 tttcgccttt cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgaagctgg
    16621 cggcatccgg ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg
    16681 tttccaaaaa cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat
    16741 cgagcaggtt ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg
    16801 cccctccttt gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg
    16861 cttccggctg caaaaccagc agatgctgct ccatgtcagc caacagttcg tccgcttcat
    16921 caaaaaatgt ctgataaaaa tcgcttatat ccatgctcac gctgtcacct cggttcggct
    16981 gatggcattg tgggaacact gacctgtggt gcaacctcag gtttttccag ggcgcttact
    17041 ggctcattct ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt
    17101 accagcaggc tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc
    17161 gttgccgcca tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt
    17221 tcgcggcggg atgcattggc ccgatcggca gaaagctccc agttgctata tcctttctca
    17281 ccgctggcgt aggggaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg
    17341 ttcagtacag gcgcaatggc acgcagaatg tcgcgcatat agggttcgac atcggcactg
    17401 ccggttctaa acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg
    17461 accagatcga ttttgagatg gggacgtaac gcccgcagtt tcggatcgga ctctatcaac
    17521 tggtcgagat caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct
    17581 tcgatgttcg gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg
    17641 ggaattgggc tttcactatt agaaatgcga tcgccgcccg taaccgcagt cgccagtgga
    17701 gtccggaagt actccgcaat ctgaatcagc tcttttgggc tggagatgga gatcagccac
    17761 atcaccagaa aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat
    17821 ccatgtgctg ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct
    17881 tgattcttca tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat
    17941 gctcttccag ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact
    18001 caacggcgat aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact
    18061 gcatcatttt gctggtttcg gcgcttttct gacgtaaaac actcgctaat ggggaaataa
    18121 atccgtaagc caataaaatg ccgaggaaag tccccaccat cgcatgtgcg ataagcgcac
    18181 ccagctcggc ggcaggacga tcggctgaac ctaacgcgtg aacgaccccc attacagccg
    18241 cgacaatacc aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt
    18301 ctgcctcgct ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt
    18361 tcatatgacc gctgataatc agacgcagat aatcgacgat aaaatcaagc atgacgctat
    18421 ccgcgaggat gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac
    18481 gttccagcga aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca
    18541 gcagatccat atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgcct
    18601 tcagcgtgcc tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac
    18661 cggcaataat caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca
    18721 aataaccgcc gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat
    18781 catccttcca ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg
    18841 ggcaaaaaaa agcagcggta cgtcgttacc gctgctggaa tgttgcgcct caccgtatca
    18901 gttaaacagc ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct
    18961 gggaaagttt acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc
    19021 tgccaacagg ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa
    19081 gttgcagtaa tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca
    19141 gtggtccttc ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg
    19201 catcgacgcc attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg
    19261 aagcatgaac gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt
    19321 tcggcggtgg gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact
    19381 gtgtttcgct ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct
    19441 gaatatcccg cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg
    19501 cagcgcttct tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt
    19561 atgaatttgc tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg
    19621 gctgtcaaaa cggaagtgac aaaccagttg attggtttct gccagcttaa ccatttgcgg
    19681 aagagtcagt gccgctaacg ttgtcgccat ttcttcattt atgccgagac gaaacatagc
    19741 ggacgctttg tcctgaacaa tcaaacgctg tgcaagtagt aaatatgaca agttgatgtc
    19801 ataaatgtgt ttcagcaact cggaggtatg cattattccc acccagaata accaacttta
    19861 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
    19921 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
    19981 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
    20041 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
    20101 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
    20161 ggtgtatttc aatctgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
    20221 ataatcgaaa tgggatgttg cgcacactta aaataactca ccgtaaataa tcatctgc
//
LOCUS       DAZGCS010000086        20246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGCS010000086 DAZGCS010000000
VERSION     DAZGCS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.086"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..1487
                     /locus_tag="ACHK01_003455"
     CDS             33..1487
                     /locus_tag="ACHK01_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO8098361.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1547..2848
                     /gene="allB"
                     /locus_tag="ACHK01_003456"
     CDS             1547..2848
                     /gene="allB"
                     /locus_tag="ACHK01_003456"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO8098362.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGEEAKREGRVTAHDYVASRPVFTEVEAIRRVLYLAKVAGCRL
                     HVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFEEIGTLAKCSPPIRDLEN
                     QKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRG
                     MSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSP
                     YVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ"
     gene            2905..4206
                     /locus_tag="ACHK01_003457"
     CDS             2905..4206
                     /locus_tag="ACHK01_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO8098363.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4228..5373
                     /gene="glxK"
                     /locus_tag="ACHK01_003458"
     CDS             4228..5373
                     /gene="glxK"
                     /locus_tag="ACHK01_003458"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO8098364.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5502..6287)
                     /gene="allE"
                     /locus_tag="ACHK01_003459"
     CDS             complement(5502..6287)
                     /gene="allE"
                     /locus_tag="ACHK01_003459"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO8098365.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6298..7533)
                     /gene="allC"
                     /locus_tag="ACHK01_003460"
     CDS             complement(6298..7533)
                     /gene="allC"
                     /locus_tag="ACHK01_003460"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO8098366.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDICIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYWVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7555..8604)
                     /gene="allD"
                     /locus_tag="ACHK01_003461"
     CDS             complement(7555..8604)
                     /gene="allD"
                     /locus_tag="ACHK01_003461"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO8098367.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8921..10588
                     /locus_tag="ACHK01_003462"
     CDS             8921..10588
                     /locus_tag="ACHK01_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8098368.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEMLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10598..11857
                     /locus_tag="ACHK01_003463"
     CDS             10598..11857
                     /locus_tag="ACHK01_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8098369.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11868..12683
                     /locus_tag="ACHK01_003464"
     CDS             11868..12683
                     /locus_tag="ACHK01_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8098370.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            12680..13573
                     /locus_tag="ACHK01_003465"
     CDS             12680..13573
                     /locus_tag="ACHK01_003465"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8098371.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKAYGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13710..14777)
                     /gene="purK"
                     /locus_tag="ACHK01_003466"
     CDS             complement(13710..14777)
                     /gene="purK"
                     /locus_tag="ACHK01_003466"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO8098372.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14774..15283)
                     /gene="purE"
                     /locus_tag="ACHK01_003467"
     CDS             complement(14774..15283)
                     /gene="purE"
                     /locus_tag="ACHK01_003467"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO8098373.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15401..16123)
                     /gene="lpxH"
                     /locus_tag="ACHK01_003468"
     CDS             complement(15401..16123)
                     /gene="lpxH"
                     /locus_tag="ACHK01_003468"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO8098374.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKAEALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16126..16620)
                     /gene="ppiB"
                     /locus_tag="ACHK01_003469"
     CDS             complement(16126..16620)
                     /gene="ppiB"
                     /locus_tag="ACHK01_003469"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO8098375.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16794..18179
                     /gene="cysS"
                     /locus_tag="ACHK01_003470"
     CDS             16794..18179
                     /gene="cysS"
                     /locus_tag="ACHK01_003470"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO8098376.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18215..18736)
                     /locus_tag="ACHK01_003471"
     CDS             complement(18215..18736)
                     /locus_tag="ACHK01_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8098377.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18844..19056)
                     /gene="ybcJ"
                     /locus_tag="ACHK01_003472"
     CDS             complement(18844..19056)
                     /gene="ybcJ"
                     /locus_tag="ACHK01_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO8098378.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19058..19924)
                     /gene="folD"
                     /locus_tag="ACHK01_003473"
     CDS             complement(19058..19924)
                     /gene="folD"
                     /locus_tag="ACHK01_003473"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO8098379.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         4633 a         5134 c         5552 g         4927 t
ORIGIN      
        1 cgttattcga tacgtgaaat taagagggat ttatggaaca tcagagaaaa ctattccagc
       61 aacgcggcta tagcgaagat ctattgccga aaacgcaaag ccagcgaacc tggaaaacat
      121 ttaactattt taccttatgg atgggttcgg ttcataacgt tcccaattat gtgatggtcg
      181 gcggcttttt tattctcggc ttgtcaacct ttagtattat gctggcaatt atcctcagcg
      241 cctttttcat tgccgcggta atggtattaa acggcgctgc gggcagtaaa tacggcgtac
      301 cgtttgccat gatcctgcgt gcttcttatg gcgtacgtgg cgcactgttt cccggattat
      361 taagaggcgg tattgctgcc attatgtggt ttggcctgca atgttatgcg gggtcactgg
      421 cctgcttgat tctgattggc aaaatctggc cgggattttt aactctcggt ggtgatttca
      481 cgctgttagg gctttctcta ccgggcttaa ttactttctt actcttctgg ctggtcaacg
      541 ttggtatagg ttttggcggt ggcaaagttt taaataaatt cactgccatt cttaacccgt
      601 gcatctatat cgttttcggc ggtatggcga tttgggcgat ttcgctggtc gggctcggtc
      661 caatctttga ctacattccg agcggtattc agaaagcaga aaacagtgga ttcttgttcc
      721 tggtggtgat taacgcggta gttgcggtct gggcggcacc ggcggtgagc gcatccgact
      781 ttacgcaaaa cgcccactcg tttcgtgagc aagcgctggg gcaaacgctg ggtttagttg
      841 tggcctatat tctgtttgcg gtcgctgggg tatgtattat tgccggagcc agtattcact
      901 acggcgctga cacctggaac gtgctggata ttgttcagcg ttgggacagc ctgtttgcct
      961 cgttctttgc ggtactggtt attctgatga cgactatttc cactaacgcc accggtaata
     1021 ttattccggc cggttatcag attgctgcca ttgcaccgac aaaactgacc tataaaaacg
     1081 gcgtactgat tgccagtatt atcagcctgc tgatctgccc gtggaaatta atggaaaatc
     1141 aggacagtat ttatctgttc ctcgatatca tcggcggaat gctgggtccg gtaattggtg
     1201 tcatgatggc acattatttt gtggtgatgc gtgggcaaat taatcttgat gaactgtaca
     1261 ccgcacctgg cgattataaa tattacgata acggttttaa cctcaccgcg ttttcagtaa
     1321 ctctggtggc cgttatttta tctcttggcg gtaagtttat tccctttatg gaaccgttat
     1381 cgcgtgtttc atggtttgtc ggcgtcatcg tcgcctttgc ggcctacgcc ttattaaaga
     1441 aacgtacaac agcagaaaaa acaggagagc aaaaaaccat aggttaatta atcccgatat
     1501 tgaacattga gttaaaaacc aatctgtatt ttacaaggag tttgttatgt cttttgattt
     1561 aatcattaaa aacggcaccg ttattttaga aaacgaagct cgcgttgtag atatcgccgt
     1621 taaaggcggg aaaattgctg ctatcggtca ggatctgggc gatgcaaaag aagttatgga
     1681 tgcgtctggt ctggtggttt cgccgggcat ggttgatgcg cacacccata tttctgaacc
     1741 gggtcgtagc cactgggaag gttatgaaac cggtactcgc gcagcggcaa aaggtggtat
     1801 caccaccatg atcgaaatgc cgctcaacca gctgcctgca acggttgacc gcgcttcaat
     1861 tgaactgaag ttcgatgccg ctaaaggcaa gctgactatc gatgcggcac aactcggtgg
     1921 cctggtgtct tacaacatcg accgtctgca tgagctggat gaagtgggcg ttgtcggctt
     1981 caaatgcttc gttgcgacct gtggcgatcg cggtatcgac aacgacttcc gtgatgtaaa
     2041 cgactggcag ttcttcaaag gtgcgcagaa gctgggcgaa gaagcgaaac gtgaaggtcg
     2101 cgtaactgcc catgactatg tggcttcgcg tccggtattt accgaagtgg aagcgattcg
     2161 ccgcgtgctg tatctggcga aagttgccgg ttgccgtctg cacgtttgcc atatcagcag
     2221 cccggaaggc gttgaagaag tgactcgtgc acgtcaggaa ggtcaggacg ttacttgtga
     2281 atcctgcccg cattactttg tactggatac cgatcagttc gaagaaatcg gcactctggc
     2341 gaagtgttca ccgccgatcc gcgatctgga aaaccagaaa ggcatgtggg aaaaactgtt
     2401 taacggtgaa atcgactgcc tggtttccga ccactcacca tgccctccgg aaatgaaagc
     2461 cggcaacatc atggaagcat ggggcggtat tgccggtctg caaaactgta tggacgtgat
     2521 gttcgatgaa gcggtacaga aacgcggaat gtctctgcca atgttcggca aattaatggc
     2581 gactaacgca gcagatattt tcggtctgca gcaaaaaggc cgtatcgccc caggaaaaga
     2641 tgccgacttc gtcttcattc agccgaatag cagctatgtt cttaccaatg acgatctgga
     2701 atatcgccac aaagtcagcc cgtatgttgg ccgtaccatt ggcgcgcgta tcacgaaaac
     2761 catcttacgt ggtgatgtga tttacgacat tgaacagggc ttccctgttg cgccgaaagg
     2821 tcaatttatc cttaaacatc agcagtaatc tggcccctgc aatgcccgtc cttgcggcgg
     2881 gcattctccg gttaaggtgt gtttatgttc aattttgcag tgagccgcga aagcctgtta
     2941 tcaggatttc agtggttttt ctttattttt tgcaacacgg ttgtggttcc tcctacgcta
     3001 ctttctgctt ttcagttgcc gcaaagtagc ctgcttacgc tcacgcaata tgcttttctt
     3061 gctaccgcac tggcctgctt cgctcaggcg ttctgcggtc atcgtcgcgc tattatggaa
     3121 gggccgggtg gcctgtggtg gggaaccatc cttactatca cccttggcga agcatcgcgc
     3181 gggacaccga tcaacgatat cgctaccagc ctggcggtgg ggattgcgct ctccggcgtg
     3241 ctgacgatgt tgattggttt tagcggatta ggccatcgcc tggcacggtt atttacgccg
     3301 tcggtgatgg tcttgtttat gttgatgctg ggcgcgcagc tgaccactat ctttttcaaa
     3361 ggtatgctcg ggctgccgtt tggcatagcc gacccaaatt ttaaaattca gttaccgccg
     3421 ttcgcgctct cggtggcggt gatgtgcctg gtactgggga tgattatctt cctgccgcaa
     3481 cgttttgccc gttatggcct gctggtcggc accataaccg gctggttgtt gtggtacttt
     3541 tgctttcctt cttcgcactc gctctccggt gagttgcact ggcagtggtt cccgctcggc
     3601 agtggaggtg ctttgtctcc gggaattatt ctgacggcgg tgattacagg tctggtgaat
     3661 atcagcaata cctacggtgc gattcggggc acggatgttt tttatccgca gcagggcgcg
     3721 ggaaatacgc gttatcgtcg cagctttgtg gcgaccggat ttatgacgct gataaccgta
     3781 ccgctggcgg taattccatt ttcaccgttt gtttcatcca ttggtttatt aacccagaca
     3841 ggcgattaca cgcggcgttc gtttatttat ggcagcgtta tttgcctgct ggtggcgctg
     3901 gttccggcac tcacgcgact gttttgcagt atccctttac ccgtgagtag cgcggtcatg
     3961 ctggtttctt atctgccttt actcttttcc gcgctggtgt ttagccagca aataacgttt
     4021 accgctcgca atatttatcg actcgcattg ccgttatttg tcggcatatt tttaatggca
     4081 ttaccgcctg tgtatctgca agaccttcca ttaacgcttc gtcctctgct cagtaacggc
     4141 ttattggtcg ggattttact ggctgttctt atggataacc ttattccgtg ggaacgtatc
     4201 gaataatttg ttgaaaaagg attgataatg aagattgtca ttgcgccaga ctcttttaaa
     4261 gagagcttaa gtgcagaaaa atgttgtcag gcaattaaag ccgggttttc gaccctcttt
     4321 cccgatgcga actatatctg tttgccgata gcggatggcg gcgaaggaac ggtggatgcg
     4381 atggtcgccg cgacgggcgg caacatcgtg acgcttgaag tctgcgggcc gatgggcgaa
     4441 aaagtgaatg ctttttatgg ccttaccggc gacgggaaaa cggcggtgat tgagatggcg
     4501 gcagcaagtg gcctgatgct ggtcgcgcct gaaaagcgta atccgttgct ggcctccagt
     4561 tttggtacgg gggagttaat tcgtcatgcg ctggataacg gcattcgcca tattattctc
     4621 ggcattggcg gcagtgcgac ggtcgacggt ggtatgggca tggcgcaggc gctcggtgtg
     4681 cgtttccttg atgccgacgg tcaggtgctg gcagctaacg gcggcaattt agcgcgcgtg
     4741 gcaagcattg agatggagga atgcgatccg cgtctggcga actgccatat tgaagtagca
     4801 tgtgacgttg ataacccgct ggtaggggca cgcggcgcgg cggcggtgtt tggcccgcaa
     4861 aaaggggcaa cgccggaaat ggtcgaagag ctggaacagg ggctgcaaaa ttacgcccgt
     4921 gttttacaac agcaaactga aatcaacgtc tgccagatgg cgggcggcgg cgctgcgggc
     4981 ggtatgggta ttgcggcggc ggtatttctc aatgcggata ttaaaccagg tattgagatt
     5041 gtgttgaatg cggtcaatct tgcgcaggca gtgcagggcg cagcactggt gattaccggg
     5101 gaagggcgca tcgactcgca aacggcaggc ggtaaagcgc cgctgggtgt ggcgtcggtg
     5161 gcgaagcagt ttaatgtccc ggtgattggg attgctggcg tattgggtga tggcgtggaa
     5221 gtggtgcacc agtacggcat tgatgcggta ttcagcattt tgccacgtct ggcaccttta
     5281 gccgaagtgc tcgccagcgg tgaaaccaat ctcttcaaca gcgcacgaaa tattgcctgc
     5341 gccattaaaa taggtcaggg aattaaaaac tgacccttac ctttaaagcg gatgcgattt
     5401 atatcgcatc tttttttacc tgaatttact acaaaaaaag ccacggtata aaccgtggca
     5461 aaatccaaca tagctaaaaa taatcaggcg agtggtatga cttaaatctc gacgtcgcgg
     5521 ttacaatctt tcgagtaaat atagctgaac gcttcaccac gccctacgcc ataaccagcc
     5581 tgtaaagaat aagcgcccat aaagatgtaa tcgccttttt tcaccgggat ccagttattg
     5641 tcgaggttat aaaccccctg accggaaaga atataggcac cgtgttcctg aacatgtgtt
     5701 tcgatataac cgtggctggc acctggtgca aaagagagga tatgcatgtt catatcaaaa
     5761 cctaactctt tgggcagaaa atccagcagg atcacatcat ccataccttc atagtgaatg
     5821 cgttccagtt cgctggcatt gccagaaacc agccacggtg catggccttc taccggaaca
     5881 tagcggcgct tatataaaaa gatttggctg tcttcggcct gggcgttaac aaacgtcatt
     5941 aaggagcctg gtgggcaata aagatagcca ccttcgctta aggcaaatgt tttgccttcg
     6001 gctttggcag tgatatttcc agagatcaca tacaggaacg tttcaatgcc ttcgccaccg
     6061 aagccctgtt ggttgccacc gttttgatgc agtgtgacca gataatcaac aaaagaggca
     6121 cccagctttg gcgtggagag gattgtcgcg tcacaatttt caaagcccgg aataatattt
     6181 tttaccagac catccggggt taacagtgcg aaattaccgt gtttaacaat cgcacggtta
     6241 gccagtaaat catcgcggta accggtgacg ttatttaaat atcccattta tgactcctta
     6301 tttctgccag gcaagttgat aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc
     6361 ggtaatattg gtgcgttccg ccgggttatg gctgatcccg ttgatgctgg ggataaaaat
     6421 catgcaggtt ggtacgcgag gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat
     6481 cacccagtaa ttcagttttt cactttcaca caattctgtc agggtggcga ccaggtcctt
     6541 attcatcggc acgggttctt cgtccatcca taaatcgata tcaatacaaa tgtccatttc
     6601 atcgcaaatc gcccgcatgt cgttttctaa ctgttgggtg aaatcacgca gcacggcagc
     6661 gtcggtatga cgacaatcaa tggtgaacgt ggttttaccc ggcaccacat ttaccgtatt
     6721 cgggcgcggc tctactttgc caaaggtcag aaccagcgga tcgcccatct ttttcgcttt
     6781 ttcgatcgac tgatggcaaa tgcgactgaa agcgtaaacc gtatcacgac gataacccat
     6841 cggcgtggtg cctgcatggt ttgattcgcc gttcagcgtc accgtatagc gacgttgccc
     6901 gacaattgca ttcaccacgc caattgactg cccattactt tccagcacac agccctgttc
     6961 gatatgcagt tcgacaaagg ctttaatatc ctgacgcgga gttagcgggg cgttcggaag
     7021 agtaaatccg caagccttca ttgcatcgac aaaactattt cctttggcat cacagatatt
     7081 ccgcacgtcg tcaggattcg ccagcccaaa gatattttta ctgccccaga agacatacgg
     7141 gaagcggctg ccttcttctt ctgccatcgc caccacttcg accgtacgta gcggcgcgcc
     7201 gtattgcgtt ttcaaccagt caattgccag ccacgccgcc agcgcgccga attgcccgtc
     7261 gaggttaccg ccgttaacca cggtatcgat atgcgaaccg ctcagaatca cttcctgtgg
     7321 atattcggta ccattcaggc gaccgtataa attccctact tcatcgaaac gtgtttccag
     7381 cccacttgct gccattcttt ttttaaattg ctgctgggtt tccagccatt ccggcgaata
     7441 aagtaaacgg gtcatcccac ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt
     7501 ttcttctatg gcttgacgaa aatgtgtaat cataagaaag tcctgtctca ataattattg
     7561 cgcaaaggga tttttcgttt cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg
     7621 gtaaatatca tcaacaattt cgatgccttc gacggcggct ttgcgttgtt taatatcctg
     7681 atcctgtccg ggataataaa cttggttaaa accgggcgcg ggggtaatgg catttaattc
     7741 gcgcatggtc tggctaagat gttgacggaa taattcgctg gaggagaaaa agttcggatt
     7801 aataactata tgtaattgcc ccaaattacg ccctgcgtgt aaatcgtcat acatcgaact
     7861 aacctgtcgc ccgaacggta agccgagtaa gacgcctgag aggacgtcaa tcatcatcat
     7921 caggccatac ccttttggcc cggcggcggg gagcagagca tgtaccgcga acggatcggt
     7981 tgttggtgca ccgtttttat cgaccgccca ggtatccggg atagacatat tacgcgagcg
     8041 ggcgtcgagc acttttcccc atgcctgtac ggtagtcgcc atatcaaagg taaggatctc
     8101 gtcgccttct cccggcgcgg caaaggccag ggggttagta ccgtagtaaa tttccgcgcc
     8161 gccaaacggc accaccattg gatcggactg gcacatcgaa atgccaatga atccggcgcg
     8221 ggctgcctgc tgcacaaaat aagagattgc gccgctgtga cccatccggc tgataccgac
     8281 caccgcaacg ccattttgct gggcggtttt gatggcatgt tccataccca ttttcgccgc
     8341 gacctgtccg gcggcattgt cggcatgtaa aattgccgag catggcccgg tttcctcaag
     8401 acgaaactcc ggttcgcggt tggtgccgcc ttttgaaatg cgttccgcgt agtattccac
     8461 gcgcaccgcg ccatgagagt ggatccctct ggcatcggcg taaaccaata cttcagccac
     8521 ggttgcagcg tgctcacgtt ttaacccagc ctggcagagt ttattctcaa ttagctggtg
     8581 gagtgtttcc cgactgattt tcatctgtct tcctttttaa cgacggtgtg aagcatgact
     8641 gcaattaaca tacagggaaa atatctggat tatgtgatcc agacaggcaa aaaaatatag
     8701 ttagaattta tttgataatc cgctcgcttt taacctggtt tttaaaacaa ccacgcttat
     8761 taaaaaataa cacgtaatag catggcggtt atttgaattc ttttgttaat aatccctgtg
     8821 tgatattcat caccttattt acccgttgtc atcgataccg taatcgccac attaacactg
     8881 ctcgtgcaat tgccatgggt gcaattttta aggagttgtt atgatccacg cctttattaa
     8941 aaaagggtgt tttcaggatt cggtcagttt aatgattatt tcacgaaaac tcagcgaatc
     9001 agaaaatgtt gatgatgttt ccgtaatgat gggtacgccc gccaataaag cgttattaga
     9061 taccacaggt ttctggcatg acgattttaa taacgccacg ccgaacgata tttgcgtggc
     9121 aattcgtagc gaagcggcgg atgcggggat cgcgcaggcg attatgcagc agcttgaaga
     9181 ggcgctaaaa caactggcgc aggggtcagg cagcagccag tccttgacgc aggtgcgtcg
     9241 ctgggacagt gcctgtcaga aattatccga tgccagtctg gcgctgattt cagtggctgg
     9301 cgagtatgcg gcggagctgg cgaatcaggc gctggatcgc aacctcaacg tgatgatgtt
     9361 ctccgataac gtcacgctgg aagatgaaat ccaacttaaa acccgcgcgc gagaaaaagg
     9421 cttgctggtg atggggccgg actgcggtac gtcgatgatt gccggcacgc cgctggcttt
     9481 tgctaacgtg atgccggaag gcaacattgg cgtcattggc gcttccggta ccgggattca
     9541 ggagctgtgc tcacagattg cgctggcagg ggagggaatt actcacgcga ttggccttgg
     9601 cgggcgcgac ctcagccgtg aagtgggcgg catcagtgcg ctgacggcgc tggaaatgct
     9661 cagtgcagac gagaaaagcg aaatgctggc atttgtttca aaaccgcctg ccgaagctgt
     9721 gcgtctgaaa attgttaatg ccatgaaagc aaccggcaaa ccgacggtgg cgctgttttt
     9781 aggttatacc ccggcggtgg cctgcgacga gaatgtctgg tttgcctcct cgctggatga
     9841 ggccgcacgc ctggcttgcc tgctttcacg cgttacggcg cgacgtaacg cgataacgcc
     9901 tgccagcagc ggatttattt gcggtttgta taccggcggt acgctggctg ccgaagcggc
     9961 gggattactt gccgggcacc ttggcgtgga agccgacgat acccatcatc acggcatgat
    10021 gctcgacgcc gatggtcacc agattattga cctcggcgat gatttctaca ccgtcgggcg
    10081 tccccatccg atgatcgacc cagccttacg caatcagtta attgccgatc tcggcgctaa
    10141 accgcaagtg cgcgtattgc tgcttgatgt cgtgattggc ttcggtgcga ccgccgatcc
    10201 tgccgcctcg ctggtaagcg cctggcaaaa agcctgtgcc gcgcgttcag acaatcaacc
    10261 actgtatgcc attgccacgg tgacaggcac tgaacgtgac ccgcaatgcc gctcgcagca
    10321 aatcgccacg ctggaagatg cagggatcgc ggtagtgagt tcgctgccgg aagccacctt
    10381 gctggcggcg gcactgattc gtccgctttc gcctgccact cagcaacaca caccgtcatt
    10441 actggaaaac gtcgccgtga ttaacatcgg attacgcagc tttgcgctgg agctacaaag
    10501 cgccagcaaa ccggttgtgc attaccaatg gtcgccagtc gccggcggca ataaaaaact
    10561 ggctcgttta ttagaacgtt tgcaataagg ggttcccatg tttacatcag tggcgcaagc
    10621 caatgctgcg gttatcgaac aaattcgtcg cgctcgtcca cactggctgg atgtgcaacc
    10681 ggcttcttca cttatcagcg aactaaacga gggcaaaaca ctgcttcacg ccgggccgcc
    10741 aatgcgctgg caggagatga ccggacccat gaaaggggcg tgcgtgggcg catgtctgtt
    10801 cgaaggctgg gcgaaagatg aagcgcaggc gctggcaata ctggagcagg gggaagtgaa
    10861 cttcattcct tgtcaccatg tgaatgccgt cgggccaatg ggcggcatta cttctgccag
    10921 tatgccgatg ctggtggtcg agaacgtgac cgacggcaac cgggcgtact gcaacctcaa
    10981 cgaaggtatc ggcaaagtga tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca
    11041 tcgctggatg cgcgatgtgt taatgccagt attaagcgcg gcgctggggc gcatggagcg
    11101 cggtatcgat ctcacggcga tgatggcgca gggcattacg atgggcgatg agttccatca
    11161 acgcaatatt gcttcctctg cactgttaat gcgtgcgctg gccccacaaa ttgctcgcct
    11221 cgatcatgat aaacagcaca tcgccgaagt gatggatttc ctcagcgtga ccgatcagtt
    11281 cttcctcaac ctcgcgatgg cttactgcaa ggcggcgatg gatgctggcg cgatgatccg
    11341 tgcaggcagc atcgtcacgg caatgacccg caacggcaat atgttcggga ttcgggtaag
    11401 cgggctgggc gaacgctggt ttactgcgcc tgtaaacact ccgcaaggtc tgtttttcac
    11461 cggcttctcg caggagcagg cgaacccgga tatgggcgat agcgcgatta ccgaaacctt
    11521 tggtatcgga ggtgcggcaa tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg
    11581 tggcatggaa gcggccagag cggtatctga agagatggcg gaaatttacc ttgagcgcaa
    11641 tatgcagttg cagatcccag gctgggattt tcagggcgcg tgcctgggac tggacattcg
    11701 tcgcgtggta gaaaccggca ttacgccact catcaatacc ggtatcgccc ataaagaggc
    11761 ggggatcggg cagattggcg caggcactgt gcgggcaccg ctggcgtgtt ttgaacaggc
    11821 gctggaagca ctggctgaaa gcatggggat tggttgagga acgcgcaatg acgatcatcc
    11881 atcctctgct tgccagccgt agtgcaccga attatcgcca gtcctggcgg ttagcgggag
    11941 tgtggcgacg ggcgattaac ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc
    12001 agggtagtgg tttcggcccc ggaggatggg tgcttcgccg cgcgcaattc gatgcgttat
    12061 gcggtggatt atgcggcaat gaacgaccac aggttgtggc tcaagggatt cgcctcgggc
    12121 gtttcacggt taaacagcca cagcgttatt gtttgctgcg tattacgccg ccttcacatc
    12181 ctcaaccgct tgcagctgca tggatgcaac gcgcggagga aaccggactt ttcggaccac
    12241 tggcgatggc ggcaagcgat ccgctgaccg ctgaattacg ccagtttcgt cactgttttc
    12301 aggccgcgct caatggcgtt aagaccgact ggcggcactg gctgggtaaa ggccccggat
    12361 taacgccaag tcatgatgac acgttgagcg gaatgctgct ggcggcctgg tattatggcg
    12421 ctttagatgc gcgctccggt cgccagtttt ttgcctgctc cgacaatctt caactcgtta
    12481 ccacagcggt gagcgtcagt tatttacgct acgccgcgca aggatatttc gcctcgccac
    12541 tcctgcactt tgttcatgct ctgagttgcc cgaaacgtac cgctgttgcg attgattcgc
    12601 tgctggcgct ggggcacacg tcaggggcag atacgctgct ggggttctgg cttggtcaac
    12661 aattattaca aggaacacca tgaaaacact ggttgtagct cttgggggca acgccttact
    12721 ccagcgcggt gaggcgctga cggcagaaaa tcaatatcgc aatatcgcca gtgctgtacc
    12781 cgcgctggcg cgcctggccc gttcttatcg gctggcgatt gttcacggca atgggccgca
    12841 ggtggggctg ctggcattgc agaatctggc gtggaaagag gtagatccgt atccgctgga
    12901 tgtgctggtg gcggaaagcc aggggatgat tggctatatg ctggcgcaga gtttgagcgc
    12961 acagccgcag atgccgcctg tcacgacggt gctgacgcgc attgaggttt cgcctgatga
    13021 tccggcgttt ttgcagccag agaaatttat tggtccggtt tatcagccag aagaacaaaa
    13081 ggcactggaa gcggcttacg gctggcagat gaaacgtgat ggtaaatatt tgcgccgggt
    13141 ggtggcgtct ccgcaaccgc gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa
    13201 agaggggcat gtggtgattt gcagtggcgg cggcggtgtg cctgtgacgg aagatggggc
    13261 agggagtgaa gcggtgattg ataaagatct cgccgctgcg ttgctcgccg agcagattaa
    13321 tgccgatgga ctggtgatcc tcaccgatgc tgacgcggtt tatgaaaact ggggaacgcc
    13381 gcagcaacgc gccattcgcc atgccacacc ggatgagtta gcgccatttg ccaaagccta
    13441 tggttcgatg gggccgaagg taacggcggt aagtggttat gtcagaagcc gtggtaaacc
    13501 cgcgtggatt ggggcgttat cgcgaattga agagacgctg gcgggcgaag cggggacctg
    13561 tatttcgctg tagtgatgag agcatccggc aatcggtgca ccatgcctga tgcgacgctt
    13621 gacgcgtctt atcaggccta caatgggtac cggagcggta agccggataa ggcgtttacg
    13681 ccgcatccgg caatcaaatg agcacctgct taactgaact tactctgcgc ccacatcacg
    13741 ccgctggcat actccggcgg cagcagcggg atcaaggctt ccagcgtcgc ggtcagacgc
    13801 gatgtgtcgc tgtcggtcaa attcagatgc cccactttac gccccggacg gacttctttg
    13861 tcgtaccagt gcagatgcac cagcggcagt ttcagccagt cataattcac atcgctacca
    13921 atcaggttga tcatcaccga cggactattc actaccggtt gcggtaacgg cagatcggta
    13981 atcgcccgca gatgcagctc aaactggctg atgctggcac cgttttgtgt ccagtgaccg
    14041 ctgttatgca cacgcggtgc cagttcgttg atcagcagac cttgcggggt gacaaaacac
    14101 tccatcgcca tcacgcccac atagcccagc tcctgcataa tcgccgacag catctcttcg
    14161 gcttgcgctt gctgctgcgc attggcctgc ggaaaagcga cgctggtgcg caaaataccg
    14221 tcctgatgca ggttatgcgt cagcggataa aacacggtgc tgccatcaaa tccgcgtgcg
    14281 ccaaccagcg acacttcacc agagaagtta atgccctgct cgacaataca ttcgccgtaa
    14341 cactctgccg gtaactgttc ggtttcattg gcacgtaaac gccattgacc gcgaccgtca
    14401 tagccaccag tgcgacgctt aacaatcgcc agttcaccta aacgctcaaa caccgcaggc
    14461 cactcgctgc gatcggcaag taactgccac ggtgcggtcg gcaggtggag cttatcgaaa
    14521 agctgcttct gagtcagacg gtcggcaata atcgggaaca catcgcggtt cacaaaggcc
    14581 ggatgacgcg ccagctcgcg ggttaatgcg gtttccggcc agcgttcgat ctcagcggta
    14641 atcacgcttt gttgaaaagg caccgccgcc ggttcagcgt ccagcccgac cggccagaca
    14701 gcaatgccta acggttcacc tgcctggcgc agcatacggc ctaactgccc gttaccgagg
    14761 acgcaaacct gtttcatgcc gcacctcgcg ggtccgggtt ttccagcact tcgtcggtct
    14821 gggctttgcg ccagtcattc agacgctggt gcagttcttt atcatgagtc gcaagaattt
    14881 gtgctgccag taacgccgcg tttgccgcgc cagctttacc aatcgccagc gtacccaccg
    14941 gaatgccgcg cggcatttgt acgatggagt agaggctatc gacaccgctc agtgcggcgc
    15001 tctgtactgg cacgcccagc actggcacca gcgttttggc ggcaatcata ccaggcaggt
    15061 gcgctgcgcc gcctgcgccc gcaataatca cctgataacc gttctcttcg gcgctttcgg
    15121 cgaagctgaa cagcttatcg ggggtgcggt gagcagaaac cacttcaacg tggtgcggga
    15181 cattcaggat ttcgaagatt tcggcggcga actgcatggt agcccagtcg cttttggacc
    15241 ccatcacgat ggcgacacgc gccggattat tgcgggaaga catgcgtctt aaaactcctg
    15301 tggtgcacaa ctctcggctt tagagggcac agagaatagc acggaaagag agcaaggaaa
    15361 acggttgcgt ggctgtgaaa tcagcaaagt tgcgggtttt ttaaaacgga aaatgaatca
    15421 gctcaacgtc atccgccgtg actttcacca tcgaaccttc cgtatgccag gcaccgagta
    15481 ccacgcgaaa agcaggttgc tggttggcga taagttcatg taccgccggg cgatgggtat
    15541 gtccgtggat cagccattgc acctgatgtt tttccatcgc actgactacc gcgttttggt
    15601 taacgtccat gatcgccagc gatttactgc tgttggcttc tttactgttc gctcgcattc
    15661 gcgcggcaat gcgtttgcgc acaaacaacg gcagggcgag gaatagcatt tgcagccagg
    15721 gtttgtggac tttggcgcga aaagcctgat aacccgcgtc atcggtgcac agcgtgtcgc
    15781 catgcataat caacacccgg cgaccataaa gttcgagcac cttttcttcc ggcaataatg
    15841 tcatgccgct ttcacgggca aagcgtttgc cgagcagaaa atcacggttg ccatgaatga
    15901 aataacaggg aacgccggaa tcggacaccg ctttgatcgc tgccgccatc tggcgatgga
    15961 gtgggttggg atcgtcgtcg ccaatccatg cttcaaacag atcgccaaga atatacagcg
    16021 cttcggcctt gcgggcttcc cccgctaaaa aacgcagaaa accggcggtg atcgccggtt
    16081 cttccacgca gagatgaaga tctgcaataa agagtgtcgc cacgattact cgctaacggt
    16141 cacgctttca atgataacgt cttcttttgg cacgtcctgg tgcataccgc tacgaccggt
    16201 tgcaacacct ttgattttgt ctaccacgtc catgccttca accacttcag caaacacgca
    16261 gtagccccaa ccttgcaggc tttcgccaga gaagttcagg aagtcgttat caaccacgtt
    16321 gatgaagaac tgtgcagttg cagagtgcgg agcctgagta cgggccattg ccagcgtacc
    16381 acgggtattt ttcagaccgt tgttggcttc gtttttgatc ggttctttgg tggctttttg
    16441 tttcatgccc ggttcaaaac cgccgccctg aatcataaag ccgttgataa cacggtggaa
    16501 aatggtgttg ttgtaaaaac cttcgcggca gtagtccagg aagtttttaa ctgtttcagg
    16561 tgctttatcg tcaaaagttt tgatgacaat atcgccgtga ttggtgtgga aagtaaccat
    16621 ttttgcatcc tgttccgttt gattggtgct tcaacccagt tcgggtcata tatagggtgg
    16681 tgttatagca taaccgcacg atcggatcat cacgcaatgt atgctgattc gcgcgggaaa
    16741 tatgggtatt atacgcaact caattaccca cacatgtcta aacggaatct tcgatgctaa
    16801 aaatcttcaa tactctgaca cgccaaaaag aggaatttaa gcctattcac gccggggaag
    16861 tcggcatgta tgtgtgtgga atcaccgttt acgatctctg tcatatcggt cacgggcgta
    16921 cctttgttgc ttttgacgtg gttgcgcgct atctgcgttt cctcggctat aaactgaagt
    16981 atgtgcgcaa cattaccgat atcgacgaca aaatcatcaa acgcgccaat gaaaatggcg
    17041 aaagctttgt ggcgctggtg gatcgcatga tcgccgaaat gcacaaagat tttgatgctt
    17101 tgaacattct gcgcccggat atggagccgc gcgcgacgca ccatatcgca gaaattattg
    17161 aactcactga acaactgatc gccaaaggtc acgcttatgt ggcggacaac ggcgacgtga
    17221 tgttcgacgt cccgaccgat ccgacatatg gcgtgctgtc gcgtcaggat ctcgaccagc
    17281 tgcaggcagg tgcgcgcgtt gacgtggtcg acgacaaacg caacccgatg gacttcgttc
    17341 tgtggaagat gtcgaaagag ggcgaaccga gctggccgtc tccgtggggc gcgggccgtc
    17401 caggctggca cattgaatgt tcggcaatga actgcaagca gctgggtaac cactttgata
    17461 tccacggcgg cggttcagac ctgatgttcc cgcaccacga gaacgaaatc gcgcagtcca
    17521 cctgtgccca tgatggccag tatgtgaact actggatgca ctcggggatg gtgatggttg
    17581 accgcgagaa gatgtccaaa tcgctgggta acttctttac cgtgcgcgat gtgctgaaat
    17641 actacgacgc ggaaaccgtg cgttacttcc tgatgtcggg ccactatcgc agccagttga
    17701 actacagcga agagaacctg aagcaggcgc gtgcggcgct ggagcgtctc tacactgcgc
    17761 tgcgcggcac agataaaacc gttgcgcctg ccggtggcga agcgtttgaa gcgcgcttta
    17821 ttgaagcgat ggacgacgat ttcaacaccc cggaagccta ttccgtactg tttgatatgg
    17881 cgcgtgaagt aaaccgtctg aaagcagaag atatggcagc ggcgaatgca atggcatctc
    17941 acctgcgtaa actttccgct gtattgggcc tgctggagca agaaccggaa gcgttcctgc
    18001 aaagcggcgc gcaggcagac gacagcgaag tggctgagat tgaagcgtta attcaacagc
    18061 gtctggatgc ccgtaaagcg aaagactggg cggcggcaga tgcggcgcgt gaccgtctta
    18121 acgagatggg gatcgtgctg gaagatggcc cgcaagggac cacctggcgt cgtaagtaat
    18181 tgcgctgttg ccggatgcga gttttcgcat ccggttatcg cctgcgccac cacaacattc
    18241 ccataagcac catccccggc aaccacaccc acatcaattc agaaattatc acctgatgcc
    18301 cgtacggcgt ggtgtaacga gacaatgcaa acggcgcgac tttaatcacc tgccagggag
    18361 cgaaaaagcg ttcatctgac cacggccaca gccagccaac gcccttaccg ccagtggtta
    18421 ccgaatccag caagctgtgc gatagcaacg agacggttaa aaacagccag cagcgaatca
    18481 gcccagccct gaaccatcgg cgtccaatca gcacacataa cagcgggaca acaaacgcaa
    18541 acaccagcga atgggtaaac ccgcgatgac caaaaacatt gccgtaagca acgccaaatt
    18601 taaacgacaa tacgtcggcg tcgggcagca tcgccaggat gattccggca aataacagac
    18661 gcggagggat gactttcgaa cccaacccta aaccaatgca taggggaacg gcggcgtgcg
    18721 taataacggt tggcatgatg gtcgcttcgg caaaatgtcg atgctatcag catggatgaa
    18781 cggggcgtag agggcaaaag tctgaaaaga gaaccggcct gttgatacag gccgggaaag
    18841 ggatcaggca acaacctgta cgctgtgacc tgcaaaactc actgtctgac cggcgacgat
    18901 tttgcagcgt ttgcgcgttt caaccgcacc gtcgactttc acctggcctt cggcaatcgc
    18961 gattttcgcc tgcgcgccgc tttcgctcca gccttccagt ttcagcaagt cgcacagctc
    19021 aacgtgcgga tgtttaccta aagaaaatgt cgccatgtta ctcatcctgt ggatcatgat
    19081 attcaacgca cgcctgtagc gtgttttcaa tcagcgtggc aaccgtcatc gggccaacgc
    19141 cgccgggaac aggcgtaatg tatgaggcgc gtttagccgc gtcttcaaac acgacgtcgc
    19201 ccacaacttt gccattttcc agacggttga tgccgacatc aatcacaatt gcgccttctt
    19261 tgatccagtc accgggaata aagcctggct tgccaacggc aacgatcaac agatcggcat
    19321 tttctacgtg atgacgcaga tttttagtga agcggtgagt cacggtagtg gtgcaacctg
    19381 cgagcaacag ttccatgctc attgggcgac cgacgatatt cgatgcgcca atcaccacgg
    19441 cgttgaggcc gaaggtatca atgttgtaac gctcaagcag cgtgacgata ccgcgcgggg
    19501 tgcagggacg cagacgcggc gcgcgctggc acagacgacc gacgttgtaa ggatggaaac
    19561 cgtccacgtc tttgtccgga tgaatacgtt ccagcacttt gacgttatca atacccgccg
    19621 gtaacggcag ttgaaccaga atgccatcga tggtgttgtc ggcattcagc gtatcgataa
    19681 gctccagcag ctccgcttcg ctggtggttt ccgggaggtc ataagagcgg gagacgaacc
    19741 cgacttcttc acaagccttg cgtttgcttg cgacataaat ttgcgatgca gggttactac
    19801 ccaccagcac aacggccagt cctggtgccc gcagtccggc tgcaatacgc gcctgaactt
    19861 tttgagcaac ttcagagcgc acctgctgcg caatcgtttt accgtcaata atctttgctg
    19921 ccatcagaga gaggattcca tctgttacgt aggtcgaagg ggatgcgtct attttgtcag
    19981 aagcggggcg cgctgtcagg tttcgtttca gatttatcgc gtgaagcgac ctcttgcgaa
    20041 ggttaggcgc accgtcgctg aggtcgctga gattgaaagc ttcatttttc gtccatgatg
    20101 gcgttgtaaa tctggaactg atttatttcc ttgtttaagg attaagataa tttaagaaat
    20161 acctgacaat ataaaaagaa ttttcagcct ggtaatttac cgcttcaggt ctatattttt
    20221 gttgaatata ttttgcgcgg aagtat
//
LOCUS       DAZGCS010000087        20137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGCS010000087 DAZGCS010000000
VERSION     DAZGCS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.087"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(136..783)
                     /gene="ychE"
                     /locus_tag="ACHK01_003474"
     CDS             complement(136..783)
                     /gene="ychE"
                     /locus_tag="ACHK01_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO8098380.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(814..867)
                     /gene="ychE"
                     /locus_tag="ACHK01_003475"
     CDS             complement(814..867)
                     /gene="ychE"
                     /locus_tag="ACHK01_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO8098381.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1260..3935
                     /gene="adhE"
                     /locus_tag="ACHK01_003476"
                     /gene_synonym="adhC"
     CDS             1260..3935
                     /gene="adhE"
                     /locus_tag="ACHK01_003476"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO8098382.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4237..4854)
                     /gene="tdk"
                     /locus_tag="ACHK01_003477"
     CDS             complement(4237..4854)
                     /gene="tdk"
                     /locus_tag="ACHK01_003477"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO8098383.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5459..5872
                     /gene="hns"
                     /locus_tag="ACHK01_003478"
     CDS             5459..5872
                     /gene="hns"
                     /locus_tag="ACHK01_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO8098384.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6016..6924)
                     /gene="galU"
                     /locus_tag="ACHK01_003479"
     CDS             complement(6016..6924)
                     /gene="galU"
                     /locus_tag="ACHK01_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO8098385.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7126..8139)
                     /gene="rssB"
                     /locus_tag="ACHK01_003480"
     CDS             complement(7126..8139)
                     /gene="rssB"
                     /locus_tag="ACHK01_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO8098386.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8231..9136)
                     /gene="rssA"
                     /locus_tag="ACHK01_003481"
     CDS             complement(8231..9136)
                     /gene="rssA"
                     /locus_tag="ACHK01_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO8098387.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            9249..9707
                     /gene="ychJ"
                     /locus_tag="ACHK01_003482"
     CDS             9249..9707
                     /gene="ychJ"
                     /locus_tag="ACHK01_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO8098388.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9757..10599
                     /gene="purU"
                     /locus_tag="ACHK01_003483"
     CDS             9757..10599
                     /gene="purU"
                     /locus_tag="ACHK01_003483"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO8098389.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10759..10843
                     /locus_tag="ACHK01_003484"
     tRNA            10759..10843
                     /locus_tag="ACHK01_003484"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10793..10795,aa:Tyr,seq:gta)
     gene            10878..10962
                     /locus_tag="ACHK01_003485"
     tRNA            10878..10962
                     /locus_tag="ACHK01_003485"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10912..10914,aa:Tyr,seq:gta)
     gene            11010..11141
                     /locus_tag="ACHK01_003486"
     ncRNA           11010..11141
                     /ncRNA_class="other"
                     /locus_tag="ACHK01_003486"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11188..11319
                     /locus_tag="ACHK01_003487"
     ncRNA           11188..11319
                     /ncRNA_class="other"
                     /locus_tag="ACHK01_003487"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11503..12180)
                     /gene="narI"
                     /locus_tag="ACHK01_003488"
     CDS             complement(11503..12180)
                     /gene="narI"
                     /locus_tag="ACHK01_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8098390.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12180..12890)
                     /gene="narJ"
                     /locus_tag="ACHK01_003489"
     CDS             complement(12180..12890)
                     /gene="narJ"
                     /locus_tag="ACHK01_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8098391.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASENLSKEDAHALG
                     IFLRDLTALDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12887..14425)
                     /gene="narH"
                     /locus_tag="ACHK01_003490"
     CDS             complement(12887..14425)
                     /gene="narH"
                     /locus_tag="ACHK01_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8098392.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKAQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14422..18165)
                     /gene="narG"
                     /locus_tag="ACHK01_003491"
     CDS             complement(14422..18165)
                     /gene="narG"
                     /locus_tag="ACHK01_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO8098393.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18681..20072)
                     /gene="narK"
                     /locus_tag="ACHK01_003492"
     CDS             complement(18681..20072)
                     /gene="narK"
                     /locus_tag="ACHK01_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO8098394.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5190 a         5279 c         4804 g         4864 t
ORIGIN      
        1 aaatccttat aactaactgt aatttgagaa gaatattttt acctattcct tttgcttttg
       61 ataactgcgc gggaacaaag taaaaataat gacgaagtaa acggagcatg gcagctccgt
      121 ttcatttgaa aggaattaat taagcaggcc ggggaaaatc cccttaatac cagtaacgat
      181 aaattcaatc cccaatgcca tcagcaatag ccccataata cgcgtaatca cgttgatgcc
      241 ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg aacaatcccc agcaacataa
      301 agcgaacaat gcaatagcca caaagaaacc aaacagatag ctaatgctgt gataacgcgt
      361 accccagacg atggtagaac tgatcgcccc tggccccgcc atcaacggca acgccaatgg
      421 caccacacca atgctttcac gtaccgcggt ttctgatttt tcttgcttgt tctgtttatc
      481 ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc accaccagga tgcccccggc
      541 gatacggaac gaatcaattg atataccaaa aagttgcaga atcgtgtcgc cgagaaaaag
      601 cgagatccac aagataatgg ccacagacag gttggctgta aggttagttt tgtttcgcgc
      661 tgccgctgtc tgataactgg tcatgctgat aaagacggga ataatcccta ccgggttgac
      721 cagcgcaaat aacccgatga aaaatttgaa gtaaacggga aaatcaaaaa aggtctgaat
      781 cacggttagc tccgaagcaa aagccggata atgttagcca taaataaggt tgaaaagacg
      841 cgctgacaat acgccttttg acagcatttt tcacctccta actacttaaa attgctatca
      901 ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta gccaccaaat catactacaa
      961 tttattaact gttagctata atggcgaaaa gcgatgctga aaggtgtcag ctttgcaaaa
     1021 atttgatttg gatcacgtaa tcagtaccca gaagtgagta atcttgctta cgccacctgg
     1081 aagtgacgca ttagagataa taactctaat gtttaaactc ttttagtaaa tcacagtgag
     1141 tgtgagcgcg agtaagcttt tgattttcat aggttaagca aatcatcacc gcactgacta
     1201 tactctcgta ttcgagcaga tgatttacta aaaaagttta acattatcag gagagcatta
     1261 tggctgttac taatgtcgct gaacttaacg cactcgtaga gcgtgtaaaa aaagcccagc
     1321 gtgaatatgc cagtttcact caagagcaag tagacaaaat cttccgcgcc gccgctctgg
     1381 ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt tgccgaatcc ggcatgggta
     1441 tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga atatatctac aacgcctata
     1501 aagatgaaaa aacctgtggt gttctgtctg aagacgacac ttttggtacc atcactatcg
     1561 ctgaaccaat cggtattatt tgcggtatcg ttccgaccac taacccgact tcaactgcta
     1621 tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat tatcttctcc ccgcacccgc
     1681 gtgcaaaaga tgccaccaac aaagcggctg atatcgttct gcaggctgct atcgctgccg
     1741 gtgctccgaa agatctgatc ggctggatcg atcaaccttc tgttgaactg tctaacgcac
     1801 tgatgcacca cccagacatc aacctgatcc tcgcaactgg tggtccgggc atggttaaag
     1861 ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc gggcaacact ccagttgtta
     1921 tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt actgatgtcc aaaaccttcg
     1981 acaacggcgt aatctgtgct tctgaacagt ctgttgttgt tgttgactct gtttatgacg
     2041 ctgtacgtga acgttttgca acccacggcg gctatctgtt gcagggtaaa gagctgaaag
     2101 ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc ggctatcgtt ggtcagccag
     2161 cctataaaat agctgaactg gcaggcttct ctgtaccaga aaacaccaag attctgatcg
     2221 gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca tgaaaaactg tccccgactc
     2281 tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga aaaagcagag aaactggttg
     2341 ctatgggcgg tatcggtcat acctcttgcc tgtacactga ccaggataac caaccggctc
     2401 gcgtttctta cttcggtcaa aaaatgaaaa cggctcgtat cctgattaac accccagcgt
     2461 ctcagggtgg tatcggtgac ctgtataact tcaaactcgc accttccctg actctgggtt
     2521 gtggttcttg gggtggtaac tccatctctg aaaacgttgg tccgaaacac ctgatcaaca
     2581 agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca caaacttccg aaatctatct
     2641 acttccgccg tggctccctg ccaatcgcgc tggatgaagt gattactgat ggccacaaac
     2701 gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg ttatgctgat cagatcactt
     2761 ccgtactgaa agcagcaggc gttgaaactg aagtcttctt cgaagtagaa gcggacccga
     2821 ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc cttcaaacca gacgtgatta
     2881 tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat catgtgggtt atgtacgaac
     2941 atccggaaac tcacttcgaa gagctggcgc tgcgctttat ggatatccgt aaacgtatct
     3001 acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc tgtcaccacc acttctggta
     3061 caggttctga agtcactccg tttgcggttg taactgacga cgctactggt cagaaatatc
     3121 cgctggcaga ctatgcgctg actccggata tggcgattgt cgacgccaac ctggttatgg
     3181 acatgccgaa gtccctgtgt gctttcggtg gtctggacgc agtaactcac gccatggaag
     3241 cttatgtttc tgtactggca tctgagttct ctgatggtca ggctctgcag gcactgaaac
     3301 tgctgaaaga atatctgcca gcgtcctacc acgaagggtc taaaaatccg gtagcgcgtg
     3361 aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt tgcgaacgcc ttcctgggtg
     3421 tatgtcactc aatggcgcac aaactgggtt cccagttcca tattccgcac ggtctggcaa
     3481 acgccctgct gatttgtaac gttattcgct acaatgcgaa cgacaacccg accaagcaga
     3541 ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg ttatgctgaa attgccgacc
     3601 acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa gatcgagaaa ctgctggcat
     3661 ggctggaaac gctgaaagct gaactgggta ttccgaaatc tatccgtgaa gctggcgttc
     3721 aggaagcaga cttcctggcg aacgtggata aactgtctga agatgcgttc gatgaccagt
     3781 gcaccggcgc taacccgcgt tacccgctga tctccgagct gaaacagatc ctgctggata
     3841 cctactacgg tcgtgattat gtagaaggtg aaactgcagc gaaaaaagaa gccgctccgg
     3901 ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt agcgctgtct ggcaatataa
     3961 acggcccctt ctggggccgt ttttttgttt acccaaagca acttttccat aaaccgacag
     4021 cattagcctt catcatattt gcgacgatgt ataacgccta aacacaggga tattgtactt
     4081 tataggtcac aagtcaacgt cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa
     4141 aaactgccga gaagggtata tagcccggaa gaagtgcgta aaacgaactg acaggataaa
     4201 agtgccctgc tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg
     4261 aatagccgtt aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac
     4321 gtatcgttca ttaccaccaa ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg
     4381 atcaagacgc agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac
     4441 cagtttgtcg gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc
     4501 ggtacgtaaa ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc
     4561 atatacttgt tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg
     4621 ctgttcatgt tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc
     4681 aggcgatgac aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc
     4741 tgccgtatat acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa
     4801 tgctgtagac ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca
     4861 caggccctca atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc
     4921 agctcaacag tatgcacaga agtgggtata tgcgttctcc cttacgaagc cttgcataat
     4981 ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg actgctggta
     5041 actattcaca atctttaacc tgttgcgcaa gtaatagccc tctgttgacc tccaggagat
     5101 agtgcaatac taagtccatg ctcttattgc gactgttcta cttttcatca ttcgcttaat
     5161 agggaattct cgtaaacaca actaatacag aagactgaaa ggtcgtcagc ctacgataat
     5221 ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg
     5281 gtgtaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag atatttattg
     5341 gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg cacaactgaa
     5401 tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga ttactacaat
     5461 gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg caagagaatg
     5521 tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta acgaacgtcg
     5581 cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata
     5641 tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa ctgctgaata gccttgctgc
     5701 cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga
     5761 cgaaaacggc gaaactaaaa cctggactgg ccaaggccgt actccagctg taatcaaaaa
     5821 agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat aatcttttgt
     5881 agattgcact tgcttaaaat cccgccagcg gcgggatttt ttattgtccg gtttaagaca
     5941 atttaataag aataagctat aaaaaaacgg cgtcgattgc tcaacgccgt ttcgtggata
     6001 acaccgatac ggatgttact tcttaatgcc catctcttct tcaagccagg ctttaaattc
     6061 cgtgccaagg gtgttatgac gaataccgta ttcaacgaag gcctgcatgt aacctaattt
     6121 attaccgcag tcatggctct tccctttcat atgataggct tccaccgttt ctttttcgat
     6181 cagcatatca attgcgtcgg tgagctgaat ttcatcacca gctcccggag gggtttttgc
     6241 cagcaacggc caaatatccg cgctaagtac gtaacgaccc acaatagcga gattagacgg
     6301 cgcaacatcc gctttcggtt tttctaccac accaaccatc ggtacgcttt cacccggcgc
     6361 taattcaacg cctttgcaat ccacaacgcc atatgcggtc acatcagcaa ccggttcaac
     6421 catgatctgg ctatgacccg tttcatcaaa gcggcggatc atctctgcca ggttatcctg
     6481 tgacaaatcg gattcatatt catccagaat aacatcaggc aaaataacag ctaccggttc
     6541 atcacccact accgggtgag cacacaatac cgcgtgtccc aggcctttcg ccagcccctg
     6601 acgaacttgc ataatagtta cgtgcggcgg acaaatagac tgcacttcat caagcagttg
     6661 acgttttaca cgtttttcca gcattgcttc cagttcaaaa ctggtatcaa agtggttttc
     6721 aatagagttt ttagatgagt gagtaaccag cacaatttca gtaatgccag ccgcaataca
     6781 ttcattcacg acgtattgaa ttaatggctt atcgacaagt ggcagcatct ctttcgggat
     6841 ggctttcgtc gccggcaaca tcctggttcc taatcccgca acggggataa cggctttttt
     6901 gactttcgta ttaatggcag ccatttaaat tctcctggac tgttcgtgtt ttgaacgtgt
     6961 tcatatttct gtatcgcatc ccagtatatc agcaccccga aggggctaag ttctgaaaag
     7021 gaggcgagtt agcataatta agacaattac ttatacgaat ggcgacgata gtaccaccag
     7081 ccgacattag caggtaatgc aaatttagcc cgcgttatcg tttgctcatt ctgcagacaa
     7141 catcaagcgc agtcgaccac cggttcccca tatttggcat tgccaggcat cgcatcgctg
     7201 gctcaattga ttcaaataag cgttacctaa agtgcctaac ggaacaccat tactgatttg
     7261 cacctggtgt tcgccggtat ttaacgtcgc attcagaccc gcagaaacca gaatgagatt
     7321 tttcagttcg cgatgataat agccaactaa tagcggaaac tgccccggca gattggcctg
     7381 acggagtaaa tggtttacct gcttcaataa cgcgcccaac tctggcaacc gttgattttg
     7441 gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg cgtaataaca aggcagcaag
     7501 tacgccatta tgtccagctc gagtgacatc aaggcaataa aatgccagat cgttttccga
     7561 aagtgcggca atatcaagca ccaggccggg tttgtccgcg gcaaccaatt gacgataatt
     7621 aacccggcaa tgggaaatca cctgctgaac cggcggttgt agttcctgta ataatttcgc
     7681 cgctgcggca gggttatcaa ccattgcatc ccagtcgcga aaaagccttt cctcttcctc
     7741 aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa accatctcgc gcaagcgatt
     7801 cagatcttta actggtttca gcaaaacatc ttcaacgccc agacgtaacg ctttggcaat
     7861 atctgccata ttttcagtgg cagatatcac cagaactggg gtctggtcgc ctctgttacg
     7921 tatatgctcc agcagtttaa gcccgttcat tcgtggcatc gcgatatcac atatcatcag
     7981 gtctggagtg aaacctccca gcaactcaag ggcatccacc ccatcagccg ccagtaccgt
     8041 tgtcgctccc aatgaggaaa accatgaatc cagaagcgag cgaaatacct gctcatcttc
     8101 aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg ttctctcccc tgactggctt
     8161 tactcaatag tggcatgcta ttgccacctg cgcctgtcag aattaactta agtgtagata
     8221 aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag ttcgtccatt ttcctttcca
     8281 ctgccagctg tccggccgca atggcagcgt gcgcgcgatg gaaatcaagc gtagatattt
     8341 gcgggcaaac aggttgaatc agaatatcgg gcggatcacc tgccatgcgg ttccttttaa
     8401 ggcggttctc cagcacctgg atagaagtgg tcataatctc tgttgccgtt ggcgctgtca
     8461 ccgcccgacg tgtcgttatg ctgcctaacc tttctttcag acgcgcatgc cacggcagag
     8521 aatcaccatt ctcgctattt tcttcactga cattaaagga gagcaaatct tgttgcatca
     8581 aatgagcatc gtgctgcagg tcaaccgcta tcacaatatc agcccccaat gcacgcgtga
     8641 gggaaatagg aattgggtta acgactgctc catcaaccag ccagtagccg ttatgtgcaa
     8701 caggtgccat gagtcctgga atactgcatg atgcgcgaat agcaagatgg agatcgcctt
     8761 cagtaaacca taattcacgt cccgtactta aattggtggc aacagccgca aagcgacggg
     8821 aacaattttc gatctctgtt tccggcatta tttcgcggta ttgattgaag acacgctcgc
     8881 cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag gcgtaaaaca tcccaataac
     8941 tgaaagaggt cacccaatct tccagcgcag ataatcgatc gcatgcatag gcagcgccca
     9001 ccagcgaacc aattgaacat cctgcaacga tatcaatttc aatacccact ttttttagcg
     9061 cattaataac gccaatatgc gaccaacctc tcgccgcgcc agatcccagc gccagcccta
     9121 tttttatctt tctcattatt cccgcaagat tcccctggaa tgcaatcgta gccacatcgc
     9181 tactgctcgg ttaacatagt gctactcagg cggttaatac gccgttattg tttccaggga
     9241 gattatttgt gtctcagctt tgtccctgtg gtagtgctgt cgagtatagc ctatgttgcc
     9301 acccttatgt gtctggtgaa aaggttgcac ctgatccaga acatctcatg cgttcgcgtt
     9361 actgcgcttt tgtgatgcaa gacgcagatt atttaataaa gacctggcat ccctcttgtg
     9421 gagcagcggc gttacgtgcc gaattgatgg ccggatttgc gcatactgaa tggttgggat
     9481 tgaccgtatt tgaacattgt tggcaggacg ccgacaatat cggatttgtc agttttgttg
     9541 ctcgatttac cgaaggtggt aaaacaggtg caataattga acgttcgcga tttttgaaag
     9601 aaaacggtca atggtactat attgacggta cacgtccgca gtttggtcgc aacgatccct
     9661 gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg ccaataatgg ttgacggtac
     9721 ggtttagcaa acactctcaa caaggttttt ccagcaatgc attcactcca acgtaaagtt
     9781 ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac gtattaccaa tatttgctac
     9841 aagcacgagt taaatatcgt acagaacaat gaatttgttg atcaccgtac cgggcgcttt
     9901 tttatgcgca cggaactgga agggattttt aatgattcca ccctgctggc ggatctcgat
     9961 agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg ccggtcgtcg ccggatagtg
    10021 attctggtca ctaaagaagc gcattgcctt ggcgatttgt tgatgaaagc caattacggc
    10081 ggcctggatg tcgaaatcgc ggcagttatt ggtaaccacg atactttacg ttctctggtt
    10141 gagcgttttg atattccgtt tgagctggta agccatgaag ggttaagccg caacgagcac
    10201 gatcaaaaga tggcggacgc cattgatgct tatcaacctg actatgtggt gctggcgaag
    10261 tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc cgaataaaat catcaatatt
    10321 caccattcat tcctgcctgc gtttatcggc gcacgtcctt atcaccaggc ctatgaacgt
    10381 ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg acaatctgga cgaaggccca
    10441 atcatcatgc aggacgttat tcatgtcgat catacctaca cagctgaaga tatgatgcgc
    10501 gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcgc tatacaaagt actggcacag
    10561 cgcgtctttg tttacggtaa tcggacgatt attctttaat cgccagcaaa aataactggt
    10621 tacctttaat ccgttacgga tgaaaattac gcaaccagtt catttttctc aacgtaacac
    10681 tttacagcgg cgcgtcattt gatatgatgc gccccgcttc ccgataaggg agcaggccag
    10741 taaaaagcat taccccgtgg tggggttccc gagcggccaa agggagcaga ctgtaaatct
    10801 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatctcaag tattacctca
    10861 aaatttgaat tacccctggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg
    10921 ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttt caaaagtccg
    10981 taaactctca aacgaatccg caattaaata ttctgcccaa gcggggaagg atgagaagct
    11041 tcgaccaagg ttcgacttga gcgccagcga gagagcgttg ccgcaggcaa cgacccgaag
    11101 ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc atcactttca aaagtccgtg
    11161 aactctcaaa cgaatccgca attaaatatt ctgcccaagc ggggaaggat gagaagcttc
    11221 gaccaaggtt cgacttgagc accagcgaga gagcgttgcc gcaggcaacg acccgaaggg
    11281 cgaagcgcgc agcgctgagt aatccttccc ccaccaccat cgcttcctta aataatcaaa
    11341 acctgttact ccgagctaat tttgcccgat ggcaccacgt ttaccgggcc tacaatatcc
    11401 tcccctgtag gtcggataag cgaagcgcat ccgacaactc accccgcacc gtgcccataa
    11461 aaaaaccccg ccgaagcagg gtctatgtga actaaaattc gcttagtgac gagcgcgcac
    11521 cagctggtac ttacgtgtca gatactccac cggtacgctc cagatgtgta ccagacgcga
    11581 gaacgggaac agcaggaata acgtcatccc cagcaccagg tgcagacgga agataaacgc
    11641 cacaccatcg aggtgttgag aagcgccacc gtggaaggtc accaccgact gcgcccagcc
    11701 aaccagtttc atcatctcgc taccgtccat atgctgagcg gagaacggaa tggtcagcag
    11761 gcccagcgcg cactggataa cgagcagcga caggatcagg atatccgctc cagtagtggt
    11821 tgcacgcacg cgtgggctga acagacgacg cttcagcaac agcacgccgc caatcagaca
    11881 cagcacgccg ctggcaccac cagcaaacat ggccattttc tgtttcactt caatcggcag
    11941 ccaggcttca tacatccagt gcggcgtcaa cataccgaag aagtgaccga caaaaatccc
    12001 cagaataccg atatggaaca ggttcgacgc caggttcatc cctttgcgat ccagcatctg
    12061 gctggacgcc gcgcgccagg tgtactgccc gtagtcataa cgcagccagc taccaatcag
    12121 gaagaccgcc ccggctatgt acgggtagat atcaaagaag aacatattca ggaattgcat
    12181 tagtgctgtc ctccggtggt gatattcaga tattgcggcg cgacggcacc ggcaaagcga
    12241 cgctgatgag cagtgattgc tgaatcaccg cagcctttgt cagcaaagaa tttaacctgc
    12301 tcttcttccc agacagcatc cagcgcctgc ggcgtatcat cgcgcgcttc gtcggcaatt
    12361 ttttccgcca ctttgtcgct gtcgatagcg gtattggcca atttcagcag cagatcaaac
    12421 agcacggcat aacggctttc acgctgttgc agacgcgcgc tcagcaatgc cagaatcggc
    12481 gcgatatctt tcaaaccttc cacggcttcg ctttgcggca gctgagccag gtactccaga
    12541 tacagcggca gatggtccgg caattcgcgg ctgtttaact gcaagccgtg ctgctcgtac
    12601 tgcgccaaca ggtcaaccat cgcctggccg cggtcgcggg attcgccgtg cacatgttca
    12661 aacagcaaca gcgacgtggc gcgaccacgg tcgaacagtt cgctgtactg cgcctgggca
    12721 tccagcggat ccagcgccgt taaatcgcgc aggaaaatgc ccagcgcatg ggcatcttct
    12781 ttcgacaaat tttccgacgc ggcaatcgcc tcaaacatct cctgttgatg ctgccataag
    12841 gcagcatccg gatattcaag gagacgcgat acaatcacga gttcgatcat ggatgcggct
    12901 ccgttttgct ggtcacatcg atggcatcga tacgacggct gttgaacaga ttgaatttgg
    12961 tatctgagcc gtggcagcca tcaccaaagg taaagccgca gccatttttc tccgggaagg
    13021 cttcccgtgc cagttcacga tgactactcg gcaccacaaa gcgatcttcg tagttagcaa
    13081 tcgccagata acggtacatc tcctgtgcct gggcttcagt caggccgacc tcttccagcg
    13141 cacgggtatc aactttaccg tcaacggttt cagcacgttt gtaatgacgc atcgccagca
    13201 tacgtttcag tgcgcgcagt accggtttgg tatcaccggc ggtcagcaga ttcgccagat
    13261 actgtaccgg aatacgcaag ctttcgacgt ctggcagaat tccgttgctg cccagctcgc
    13321 ccgcgtctgc tgcagactga atcggagaca gaggcggcac gtaccagacc atcggcagtg
    13381 tgcgatattc tggatgcagc ggcagcgcca gtttccattc catcgccatt ttataaaccg
    13441 gcgactgctg tgcagcttca ataacgctca gcggaatacc gtctttaatc gcctgctcga
    13501 tgactttcgg atcgttcgga tcgaggaaca cgtccagctg acgctggtag agatctttct
    13561 cgttctcggt gctggctgca cgttcaatcg cgtcggcatc gtacagcagc acgccaagat
    13621 aacggatacg accgacacag gtttctgagc acacggtcgg ctgacccgct tcaatacgcg
    13681 gatagcagaa gatgcacttc tcagacttac cgctcttcca gttgaagtag atttttttgt
    13741 acgggcatcc ggtgatgcac atacgccagc cacggcattt atcctggtcg atcaggacga
    13801 tgccatcttc ttcacgcttg taaatcgcac cgctcgggca ggtcgccaca catgccgggt
    13861 tcaggcagtg ttcgcacagg cgcggcaaat acatcataaa ggtgttttcg aactggctat
    13921 acatcgcctt ctggatgttg tcgaagttct tgtctttcgc cagtttgtca aactcaccgc
    13981 ccaggtcatc ttcccagttc ggcccttttt cgattttcgc catccgttcc ccggtaatca
    14041 gcgaacgcgg acgggcaatc ggctgcgctt tgctgccttc cggcgcggta tgcaggttct
    14101 gatagtcaaa atcgaacggc tcgtaataat cgtcgatccc cggcagatgc gggttagcga
    14161 agattttacc cagcagcatg gcgcggttac ccatgcgcgg ctgcagtttg ccattgattt
    14221 tacggatcca gccgcctttg tatttttcct ggttttccca gtcagtcggg aagccctggc
    14281 ccggcttggt ttccacgttg ttgaaccacg cgtattccac gccttcacgg ctggtccaga
    14341 cgtttttaca ggtaactgaa caggtgtggc agccgatgca cttatcgaga ttcagcacca
    14401 tgccgacttg tgaacgaatt ttcattttac gctctcctgt acctggtcat tgccttcgcc
    14461 atctaaccag tcaatgttct tcatcttacg cactacaaca aactcatcgc ggttagaacc
    14521 tacggtgcca tagtagttaa agccgtatgc cagatgggca tagccgccga tcatatgcgt
    14581 cggtttcggc gtgatacggg tgaccgagtt atggatacca ccacgctgtt gggtaatttc
    14641 cgaaccaggc aggttaacga tacgttcctg cgcgtggtac atcatggtca tccctgccgg
    14701 aacacgctgg ctgacaaccg cacgggcagt cagagcaccg ttgctgttga atacttcaat
    14761 ccagtcgtta tcggcgatac ccagatcttt ggcatcggct tcactcaacc agaccaccgg
    14821 accaccgcga cctaaagtca gcatcagcag gttgtcgctg taggtggagt ggatacccca
    14881 cttctggtgc ggcgtcagga agttgagcgc tttttccggg ttgccattgg atttctggcc
    14941 catcacttct ttcaccgaac gggtgtcgat cggcggacga taaaccagca ggctttcacc
    15001 gaaatcacgc atccactggt gatcctgata cagttgctga cgaccagaga gcgtacgcca
    15061 cgggatcagc tcgtgaacgt tggtgtaacc ggcgttgtaa gaaacgtgtt catcttccag
    15121 accagaccag gtcgggctgg agatgatttt gcgcggctgt gcctgaatat cgcggaagcg
    15181 aatcttctcg tcttctttat tcagcgccag atgcgtatgg tcacgaccgg taaactcgct
    15241 cagggcagcc caggctttca cggctacctg accgttggtt tccggtgcca gcgtcaggat
    15301 catctctgcc gcatcaattg cggtgttcag catcggctgg ccttttgccg gaccttccgc
    15361 tttggtgtag ttgagcttac gcagcagatc catctcgctc tgggtgttcc aggcaatacc
    15421 cttaccaccg ttaccgattt tctccatcag cgggccgata gaggtaaagc gttcgtaagt
    15481 tgccggataa tcgcgctcta cgaccataat gtgtggcgcg gttttacctg ggatcaggtc
    15541 gcattcgcct tttttccagt ctttcacatc cagcggctgc gccagttcag cggcagagtc
    15601 gtgctggata ggcagcgtga cgatgtcggt ttctttaccc agatggccga cgcacacttc
    15661 ggagaatttc ttcgcgatgg ctttgtagat ttcccagtcg cttttcgctt cccaggccgg
    15721 atcgaccgcc gcagacagcg ggtgaataaa cggatgcata tccgaagtat tcatgtcgtc
    15781 tttttcgtac caggtcgccg tcggcaaaat gatgtcggaa tagagacagg tgctcgacag
    15841 acggaagtcc agcgtaacca ccagatccag cttgccttcc agaccattgt cctgccagtc
    15901 cacttcttcc ggcttcacgc cgccctgctg ccccagatct ttaccctgga taccgtgctc
    15961 cgtccccagc aggtacttga gcataaactc atgaccttta ccggaagaac cgagcaggtt
    16021 agaacgccag atgaacaggt tgcgcgggtg gtttttaccg ttttctggtt gttctgccgc
    16081 aaaacggatg gaaccctctt tcagggattt cacggtgtaa tctaccggat tcatcccggc
    16141 tttttccgct tcgccagcga tagtcagcgg gttagtgcct aactgcggtg cagacggcag
    16201 ccagcccatg cgttctgcac ggacgttaaa gtcgattagg tgtccggtat aacgggattt
    16261 atccgccatc ggcgacagca gttcttccgc agtcacggtt tcataacgcc actggctgga
    16321 gtggttatag aaataagaag tgctgttcat gtgacgcgcc ggacgctgcc agtcaagggc
    16381 aaacgccagc ggctgccagc cggtttgcgg acgcagtttt tcctgaccta catagtgcgc
    16441 ccagccgccc ccgctctgac cgacacagcc gcagaaaatc agcatgttga tcagaccacg
    16501 atagttcata tcgaggtgat accagtggtt cagccccgca ccgacgataa tcatcgaacg
    16561 accgtgcgtt ttatcagcgt tatcggcaaa ttcacgggcg atgcgaataa tctggctgcg
    16621 agaaacgccg gtaatctgct cggcccaggc cggggtataa gctttcacat cgtcatagct
    16681 ggttgcacag ttaacgtcgt tcaggccacg ttccagaccg tagtttgcca gcgtcagatc
    16741 ataaacggtg gtcaccaggg cggtgctgcc atcagccagt tgcaggcgtt tcaccggcag
    16801 tttgtgcagc agcacgtttt ccagttccac tttgttgaag tgttcagtac cgtcgccacc
    16861 aaagtacggg aagcccacct cagcgatctc atcctggcta cccagcaggc tcagttgcag
    16921 ctcggtttct tcgccagttt tgccgtcgcg ctgctcaaga ttccatttgc ccttctcgcc
    16981 ccagcggaag ccaatagaac cgttcggcgc aaccatttca ccattggtat taaaggcgac
    17041 agttttccat tccggattgt tttcctgacc cagcgcatcc accagatcag cagcgcgcag
    17101 catacgaccc gcggcgtagt aaccgtcgcg ctcttccagc atcaccagca tcggcatgtc
    17161 ggtgtagcga cgcacatagt cggtgaaata ctggcttggg ttgtcgaggt ggaattcacg
    17221 cagcattacg tggcccattg ccagcgccat tgccgcatcg gtgccctgtt tcggtgccag
    17281 ccacagatcg cacagtttgg cgatttcagc gtagtccggc gtgacggcaa cagttttggt
    17341 ccctttgtaa cgcacttcag taaagaagtg agcatccggg gtacgcgtct gcggcacgtt
    17401 tgacccccag gcgatgatgt agctggagtt gtaccagtca gcagattccg gtacgtctgt
    17461 ttgctcgccc caggtttgcg gagacgcagg aggcaggtcg cagtaccagt cgtagaagct
    17521 taagcaagta ccgccaatca gcgagagata gcgtgcaccc gatgcgtaag aaaccatcga
    17581 cattgccgga attggcgaga agccagcgac acggtccggg ccgtagtttt tgatggtgta
    17641 aacgttagat gcggcgatca gttcgttcac ttcctgccag gaagaacgaa caaaaccacc
    17701 gcgtccacgc gcctgcttaa agcttttcgc tttatcggcg tcttcaatga tagaagccca
    17761 tgcctcaacc ggatcgctat gcagcgcctt cgcttcacgc cacattttca tcaagcgttt
    17821 gcgcatcatc gggtatttca ggcggttggc gctgtaaaga taccaggagt agctggcacc
    17881 gcgcgggcag ccgcgaggtt catggtttgg caggtccggg cgagtacgtg gatagtcagt
    17941 ctgctgggtt tcccaggtga ccagaccgtt tttgacgtag attttccagc tgcaggagcc
    18001 ggtgcagttt accccgtggg tagagcggac gattttgtca tgctgccaac gctggcgata
    18061 tccatcctcc cagtcacggt tggtattgag aagctggcca tgcccatcgg caaaggtttc
    18121 acccttctgt ttgaagtagc gaaaccggtc caggaattta ctcatcggtt ttctcctgtg
    18181 ggagcctgtc ggccctctga taaatcgaca ttgctaatgt ttaagggcaa ggtaacgctc
    18241 tgaaacagcg tgggaattga taacgatcaa gtgtgatggg gatgaaaaat aaagtaaatt
    18301 cctaagatac ccctttagga taattaacag ttaaagcata acatgctgta aattaagacg
    18361 ttttacacct gtagttattt aaactattaa gaattttatg cagatgggta ttaaggagta
    18421 ttccccatgg gtaacatatt aatggctctt aatgaaatcc ggcgaaataa accatacaac
    18481 attagtatgt tgtaactaaa tgacgcataa aaaaagcgcg acatcatgcc gcgcaaagga
    18541 taatcaaata ttttactcta tataattcgg tttacaggaa ggcggcaagt gaatgaatcc
    18601 caggagctta ctaaagtaag tgactggggt gaatgaacgc agccgcagca catgtaaccc
    18661 gaagtatgac gagtaacggt ttatttttta gaatgccgac catataccgc ccaggtaatc
    18721 actacgcagg cgatatagaa aatcaaaaat actttcattg cgccgactgg cgaacccgtt
    18781 aatgccagcg agctaccaaa cgctttcggg ataaagaagc caccaatcgc gccaatcgca
    18841 gagatgaaac ccaacgccgc cgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca
    18901 gaaccccctt ctgctttcac gcgatccatt gtcagtttac ggaagatcac tgaaatcatc
    18961 tggaaagtgg aaccactacc cagcccagct gtcaggaaca gcgccaggaa gaccgcgaag
    19021 aacgccataa agcttccgcc ctgcccgtca gtcggtaagg tcaggaacag caggccgctg
    19081 aaaatcgcca tcagaataaa gttcaccagc gtgacacgag ttccgcccag acggtcagat
    19141 aatgcaccac ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa agcgtattgc
    19201 agaatctgaa catccgggaa ctgcgttttt gacagcatcg caaagcccgc ggagaagccg
    19261 ataaaggagc cgaaggttgc cagatacagc aggctcataa tccacagatg cccccgtttg
    19321 agtaccggca actgctcctt gatggaggct ttcgaggtag caagatcgtt catgccaaac
    19381 cacgccgcaa tggtgaagat ggcaaggaac ggcacccata tccaggacgc attcgccaga
    19441 tacagctcag tcccatccgg ctgtttgacg ccctggctac caaatactgc gaaaatcgac
    19501 agtgatacca ccagcggagc aaccaactgc atgacgctga cgcccatgtt gcccagacca
    19561 ccattcagac ccagcgcgcc accctgcttc tgtttcggaa agaagaagct gatgtttgcc
    19621 atactggagg cgaagttcgc gccagcaaag ccgcacagca gagagatgat gatgaagacg
    19681 ctataaggcg tggaggtatc ctgcacggca aaacccagcc agacacaagg aataatcaga
    19741 ataccggtgc tgaacgccgt ccagcgacga ccaccgaaga taggaaccat aaaggagtat
    19801 ggaacacgta ataacgcgcc agaaactgaa ggcagcgcag tcaacataaa tagctgatcg
    19861 gtcgtaaaat taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa caacatccat
    19921 acacaaaacg ccagcagcag acagggaacg gaaatccaca ggttgcggct ggcaatacgt
    19981 tgaccgcgtt gttgccagaa cgcaggatct tccggtcgcc aatctgtaat gacagctcca
    20041 gtagcccttt cgggggcgga tgagtgactc atagacacct ctgatactcg tttcgatgtc
    20101 tgccacctta gtgtctgtag ctaaaggcaa tttgatg
//
LOCUS       DAZGCS010000088        20120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGCS010000088 DAZGCS010000000
VERSION     DAZGCS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.088"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..111
                     /locus_tag="ACHK01_003493"
     CDS             <1..111
                     /locus_tag="ACHK01_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8098395.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            173..442
                     /locus_tag="ACHK01_003494"
     CDS             173..442
                     /locus_tag="ACHK01_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8098396.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            411..1529
                     /locus_tag="ACHK01_003495"
     CDS             411..1529
                     /locus_tag="ACHK01_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO8098397.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1606..2073)
                     /locus_tag="ACHK01_003496"
     CDS             complement(1606..2073)
                     /locus_tag="ACHK01_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO8098398.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(2051..2254)
                     /locus_tag="ACHK01_003497"
     CDS             complement(2051..2254)
                     /locus_tag="ACHK01_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098399.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            2563..3357
                     /gene="potC"
                     /locus_tag="ACHK01_003498"
     CDS             2563..3357
                     /gene="potC"
                     /locus_tag="ACHK01_003498"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO8098400.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3354..4400
                     /gene="potD"
                     /locus_tag="ACHK01_003499"
     CDS             3354..4400
                     /gene="potD"
                     /locus_tag="ACHK01_003499"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO8098401.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4458..4919
                     /locus_tag="ACHK01_003500"
     CDS             4458..4919
                     /locus_tag="ACHK01_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO8098402.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            4916..5704
                     /gene="ycfZ"
                     /locus_tag="ACHK01_003501"
     CDS             4916..5704
                     /gene="ycfZ"
                     /locus_tag="ACHK01_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO8098403.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKALFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5843..6664)
                     /gene="cobB"
                     /locus_tag="ACHK01_003502"
     CDS             complement(5843..6664)
                     /gene="cobB"
                     /locus_tag="ACHK01_003502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO8098404.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6680..7591)
                     /gene="nagK"
                     /locus_tag="ACHK01_003503"
     CDS             complement(6680..7591)
                     /gene="nagK"
                     /locus_tag="ACHK01_003503"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO8098405.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7620..8864)
                     /gene="lolE"
                     /locus_tag="ACHK01_003504"
     CDS             complement(7620..8864)
                     /gene="lolE"
                     /locus_tag="ACHK01_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO8098406.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8864..9565)
                     /gene="lolD"
                     /locus_tag="ACHK01_003505"
     CDS             complement(8864..9565)
                     /gene="lolD"
                     /locus_tag="ACHK01_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO8098407.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9558..10757)
                     /gene="lolC"
                     /locus_tag="ACHK01_003506"
     CDS             complement(9558..10757)
                     /gene="lolC"
                     /locus_tag="ACHK01_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO8098408.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11019..12092
                     /gene="ycfT"
                     /locus_tag="ACHK01_003507"
     CDS             11019..12092
                     /gene="ycfT"
                     /locus_tag="ACHK01_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO8098409.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFTPPSLPAAVSYSR"
     gene            12220..15666
                     /gene="mfd"
                     /locus_tag="ACHK01_003508"
     CDS             12220..15666
                     /gene="mfd"
                     /locus_tag="ACHK01_003508"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO8098410.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEATNLLPAHEFP
                     TDKAAIELFRSQWRDIFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQTDTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15810..16772
                     /gene="ldtC"
                     /locus_tag="ACHK01_003509"
     CDS             15810..16772
                     /gene="ldtC"
                     /locus_tag="ACHK01_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO8098411.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16855..17112)
                     /gene="bhsA"
                     /locus_tag="ACHK01_003510"
                     /gene_synonym="ycfR"
     CDS             complement(16855..17112)
                     /gene="bhsA"
                     /locus_tag="ACHK01_003510"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO8098412.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            17353..17985
                     /gene="comR"
                     /locus_tag="ACHK01_003511"
     CDS             17353..17985
                     /gene="comR"
                     /locus_tag="ACHK01_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO8098413.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(18047..18586)
                     /gene="ycfJ"
                     /locus_tag="ACHK01_003512"
     CDS             complement(18047..18586)
                     /gene="ycfJ"
                     /locus_tag="ACHK01_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8098414.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(18796..20100)
                     /gene="ndh"
                     /locus_tag="ACHK01_003513"
     CDS             complement(18796..20100)
                     /gene="ndh"
                     /locus_tag="ACHK01_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO8098415.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         5089 a         5188 c         4983 g         4860 t
ORIGIN      
        1 gctattccat tcgaaggtga gcgccataat gcacttgatg acgcccgtta ccaggcaaaa
       61 tacgtttcag ttatctggca aaaactgatc ccgagtcagg ctgattttta atgttcaacc
      121 gtcgccagtt gtcgttgata ttctgcaact ggcgcgttcc ggagtgatag ccatgagcga
      181 acagtacctg ataacgctcg acgagtggaa accaaaacgg ttcagtctcc caataacaaa
      241 cactaccctg gtgaaatacg gaaaactagg atacatcgtt ccaagaccac aaaaaattcg
      301 tgggcgttgg ctgatagatc gccgagcagt atttgttggg cctggtgaaa cgggaattgc
      361 gccggaaatt catactggcg atgatgatgc actgaaggag attttaactc atgtcaccga
      421 ggccacgaaa aaacagcact gacgtagccg gtctttacga aaagtttgat cgcagaactg
      481 gcagagttta ctaccagtat aaaaatcctg tgactggaaa atttcacgga ctcggaacag
      541 acaaaggtaa ggcagaaaaa atcgcttcca cagccaatca gcggatagct gcagcagaag
      601 ctgaatattt catgcgcaaa attgatgaaa gtccgtcagc aacaaaacgt cggggtatca
      661 gattaaaggc atgggttgat cgatatctga aaatacagga cacgcgactg aaaaatggag
      721 atattgcagc tacaactcac aaagaaaaag cccgaatggc tgcatacctg gtttcccgtc
      781 tgggaaacca cccattgaaa gaactggaag taagagactt tgcattaata ctggatgagt
      841 ggctggataa agacatggtc agcacagcga gagtaaatcg tggattatgg gttgatattt
      901 ataaagaagc acagcatgca ggggaagttc ctcctggatg gaatcctccg gaggctaccc
      961 gtaaaccgat ccctaaagta actagagcca ggctcaccct ggaagactgg caaaaaattt
     1021 acaacgcaac gcctgaaaaa cactttatcc gtaacgcaat gcttcttgcg attgttactg
     1081 gtcagcgccg tgatgacatt tgccacatgc gtttttcaga tgtgtggaac gaacacttgc
     1141 atatcaccca gggaaaaacc ggaatgcgtc tggcgttacc gcttacacta cgctgtgatg
     1201 ccattgggat aacgttaaaa gaagttattg atgggtgccg agacagaata ttaagtccat
     1261 atctaatcca tagtcggcac cagaaacaac caaagccgat gagtaaagac aacctgagcg
     1321 actactttgc caaagcacgg gatctggctg ggataactcc accagcagga aaaactccgc
     1381 caacatttca tgaacaacgc tccctgtcag aacgactgta ccgcgcgcag ggtattgata
     1441 caaaaacatt actgggacat aaagtccagg caaccaccga tcgttataac gatactcgtg
     1501 gtcaggaatg ggttaaattg gttgtttgac gaaataaatg cagctgaaaa atgttgatat
     1561 tgctttgcta gtgtaaaaac cccggcatca accggggttc ggagactaat cttgatcagg
     1621 ttcctgctta gaataatgtt ttccaacaat aaatgccgta accaatgcaa caagatctat
     1681 tgaaacgaga gttccggcaa gaactgtctc ccccatgacg ccaaagacag tggcagctaa
     1741 gatgatcaat attgccaacc aaaaagcctt tgtttgacca tctctggcaa tatcaatgct
     1801 atcagccact gttttatggc gatgagcctg ctctttttcg gtcaactcaa caagtcgatt
     1861 tgcaagccca ggcaccagtt gatcatactt tttaagcatt gaaggtggcg gaacaggccc
     1921 ctgaaaatgc tggcaaacaa tagcccgcac ctgcggacta tccaaaaccc tgtttaagac
     1981 ctctggattt tcgattactc gagaaaccag ttcattatcc ttttgttctt cacaagtgag
     2041 tttggtgttc tcactttctt tttgatctgg cattgttcag cttttcaacg ttaacggata
     2101 ccattgcttt ttggaaatcg cctctgatag ccgcagcatc ctgacgcaaa gcggaaactg
     2161 gattcgctgg catgccattt gctgaaacga ccgtcgggct cagggctata accgagccaa
     2221 tggcttgtaa caaagccaca cttttacgct tcatagtcac tcctgagttc aatgggattc
     2281 aaaacggatt caaagtgaat cttgatagct aattctaata ccacattatg tggtttttca
     2341 acacgaagaa atcacatcaa aaatctcatt cggaaagcga tatgggtaaa caaactggaa
     2401 tacttttctg tcctagtaag ctcacccatg tataaaaatg ctgtaaaatg cgtggtcctg
     2461 ttttggggaa aagttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     2521 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     2581 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     2641 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     2701 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2761 gcggtgtttt cggcgacgtt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2821 cgttatcgct ttcgcggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtcg
     2881 ccagatattg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2941 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     3001 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     3061 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     3121 gcgggctggg tgttaagctt taccctgtcg atggacgatg tagtagtttc ttcgttcgtc
     3181 accggaccaa gctatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     3241 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggtaatt
     3301 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     3361 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     3421 ccgatgacaa caatacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     3481 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     3541 aaaccatgta cgccaagttg aaaacgtaca aagacggtgc ctacgacctg gtggttcctt
     3601 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     3661 agttaagcaa tttcagcaac ctcgatccag acatgctcaa caagccgttt gacccgaata
     3721 acgactactc cattccgtat atctgggggg cgacggcgat tggcgttaac ggtgatgcgg
     3781 tagatccgaa atccgttacc agctgggccg atctgtggaa gcctgagtac aaaggtagcc
     3841 tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgcaaa ctgggctact
     3901 ccggtaacac taccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3961 tgccaaacgt cgcagcgttt aactccgata acccggctaa cccgtacatg gaaggcgaag
     4021 ttaacctcgg catgatctgg aacggctctg ctttcgttgc acgccaggcg ggtacgccaa
     4081 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     4141 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     4201 tggcaaaaca ggttgccgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     4261 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     4321 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     4381 agctgaaagc aggacgttaa ttcagcaaac gggcggtggt attaccgccc gttttttagt
     4441 tagcaaggat gtggctaatg agtcaggaca gtaaagtttt atcccgcgct tttctcggta
     4501 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     4561 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     4621 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     4681 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4741 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4801 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4861 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc cgcccatgaa
     4921 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4981 attgattcca ataatgaagg cactttttac ggatgttacc ggtactgtac cggatgctga
     5041 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     5101 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     5161 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     5221 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     5281 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     5341 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     5401 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     5461 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     5521 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     5581 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     5641 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     5701 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5761 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5821 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5881 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5941 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     6001 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     6061 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     6121 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     6181 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     6241 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     6301 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     6361 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     6421 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     6481 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     6541 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     6601 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     6661 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6721 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6781 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6841 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6901 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6961 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     7021 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     7081 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     7141 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     7201 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     7261 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     7321 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     7381 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     7441 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     7501 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     7561 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     7621 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     7681 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7741 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7801 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7861 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7921 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7981 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     8041 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     8101 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     8161 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     8221 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     8281 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     8341 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     8401 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     8461 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     8521 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     8581 gcagcggcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     8641 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     8701 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8761 gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg tcgccgtccg
     8821 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8881 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8941 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     9001 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     9061 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     9121 cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca
     9181 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaataagc agcggcatag
     9241 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     9301 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     9361 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     9421 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     9481 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     9541 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     9601 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     9661 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9721 atcggcatca gattatttaa ctggctggca agcagggcgc caagcgccgc tccgaggatc
     9781 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9841 gttaagcctt gcgtttgcag gatcgctact tcgccctgct tctccattac catcagccct
     9901 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag taaacccatc
     9961 atattttttt ccatgcgtac ggcctggaac aactcgcctt tacgatcacg ccagtcctgc
    10021 catttgctgc cttcaggcag tttttgctga cttaatgagt cgactttcag cggctcatcc
    10081 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
    10141 tcaatattca ccagcatttc attgccatcg acttcactgt tggcggcgaa agtgccaatc
    10201 acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggt
    10261 accatcatgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
    10321 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc
    10381 gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
    10441 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
    10501 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
    10561 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
    10621 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
    10681 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10741 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10801 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10861 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10921 tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata acccgcagga
    10981 tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    11041 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    11101 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    11161 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atttccggct
    11221 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    11281 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    11341 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    11401 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    11461 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    11521 tcgccttgtt tgtactgttg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    11581 tgaacagtgt gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacaa
    11641 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    11701 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11761 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11821 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11881 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11941 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    12001 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc accccgcctt
    12061 cactgcccgc tgccgtcagt tactcccgct aagccatcgc cccagccggg gcgattcaat
    12121 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    12181 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    12241 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    12301 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    12361 gatatgcaaa atgctctgcg tttgcatgat gaaatcagtc agttcaccga tcagatggtg
    12421 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    12481 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    12541 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    12601 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    12661 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12721 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12781 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12841 gcgaccaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12901 cgcagccagt ggcgcgatat cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12961 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    13021 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    13081 ctggaaacca gtgccgaacg tttccagact gacacgctgg cgcgttttga gaatcgcggc
    13141 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    13201 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    13261 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    13321 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    13381 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    13441 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    13501 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    13561 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    13621 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    13681 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13741 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13801 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13861 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13921 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13981 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    14041 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    14101 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    14161 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    14221 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    14281 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    14341 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    14401 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg
    14461 aacgtggata ttctgacgct tactgcaacg ccgatcccac gcacgctgaa tatggcaatg
    14521 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    14581 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    14641 cgcggggggc aggtttatta tctctacaat gatgtggaaa acatccagaa agctgccgaa
    14701 cggctggcag aactggtgcc tgaagcacgg attgccatcg gtcacgggca gatgcgcgag
    14761 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14821 accaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgt
    14881 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcgcat
    14941 catcaggcat atgcatggtt gctgacaccg catccaaaag cgatgactac cgatgcacaa
    15001 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    15061 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    15121 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    15181 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    15241 atgccgtcgc tattgccaga tgatttcatc cctgacgtga acacgcgtct gtcgttctac
    15301 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    15361 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    15421 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    15481 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    15541 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    15601 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    15661 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15721 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15781 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15841 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15901 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15961 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    16021 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    16081 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    16141 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    16201 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    16261 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    16321 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    16381 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    16441 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    16501 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    16561 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    16621 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    16681 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16741 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca
    16801 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16861 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16921 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16981 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    17041 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    17101 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    17161 atcgataact aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc
    17221 attaattaat ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga
    17281 tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg
    17341 tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca
    17401 aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt
    17461 tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta
    17521 tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat
    17581 cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg
    17641 ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc
    17701 tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg
    17761 ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa
    17821 gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt
    17881 attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actaatgcag
    17941 attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc
    18001 cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat
    18061 tcaaaatcag ttgcccatta ctatctagcg ggatctgcgt acccggatcg cggtccatgc
    18121 ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg
    18181 acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga
    18241 gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga
    18301 catctttacc gcgaccaccg ccaaactgat gccctatcac gccgccagca acagcgccga
    18361 gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt
    18421 tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct
    18481 gagcgtattg cgggccacgt tcaaacacgt tcagactggc cactgccgct acgcccagcg
    18541 cagctgcgac accaatcccg atacccgcca acattgattt attcacggga acacctcctt
    18601 ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga
    18661 aaagcgcggg aatggctgga aatacttaag gattcggagg agtctgaagg aatgcctgat
    18721 gcgacgcggc tgccgatcgc gctgttcctc aagggcggat aaggcgttta tgccacatcc
    18781 gccagtgtac gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc
    18841 caccagcatc attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta
    18901 tagcgagata tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag
    18961 gttacccatc aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc
    19021 tttatactga taatttttca gcggcttacc attcatctgc gccagaatgt tgttcattgc
    19081 gcaagtcgcc atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg
    19141 cgggcatgac gcgcagtcgc caatagcgta aatgtctgga tcgcgggtgg tttgcagcgt
    19201 cggttccacc accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa
    19261 gtctggcgct ttgatcccgg ctgcccacac catcagatca gcctcaatat attcgccatc
    19321 tttagtgtgc aggccgcctt tatcagcact ggtgaccatg gtttgcgtca gcacgcgaac
    19381 gccaagtttc gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa
    19441 aatacgttct cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta
    19501 accgtagctg tgcagttgct tgaccgcgtt gtgcaattca gcggagagtt ctacacccgt
    19561 cgcgccgccg ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcggagta
    19621 tttcaggaag agattcagca tctcctggtg aaagcgacgc gcctggtgcg ggttatcgag
    19681 gaaaatgcag ttctctttga cacctggcgt attgaaatcg ttagaggtgc tacccagcgc
    19741 cattaccagg gtgtcatagg cgattttacg ttccggaact agcagttcac ctttctcatc
    19801 gcgcagttct gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccag
    19861 ctggaactgg aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc
    19921 atcaagcgag ccagtcgcca cttcgtgcag cagcggtttc cacagatggc tgtggttacg
    19981 atcgaccagc gtaattttgg cttttttctt gcgtcccagc ttatgcccca gctgtgttgc
    20041 catttccagc ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa
    20101 cgtgaccccc ttaaatttat
//
LOCUS       DAZGCS010000089        20115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGCS010000089 DAZGCS010000000
VERSION     DAZGCS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.089"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..205
                     /locus_tag="ACHK01_003514"
     CDS             <1..205
                     /locus_tag="ACHK01_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8098416.1"
                     /translation="NIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
     gene            207..476
                     /locus_tag="ACHK01_003515"
     CDS             207..476
                     /locus_tag="ACHK01_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8098417.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(622..1212)
                     /gene="tccP2"
                     /locus_tag="ACHK01_003516"
                     /pseudo
     CDS             complement(622..1212)
                     /gene="tccP2"
                     /locus_tag="ACHK01_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
     gene            complement(1537..1726)
                     /locus_tag="ACHK01_003517"
                     /pseudo
     CDS             complement(1537..1726)
                     /locus_tag="ACHK01_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1815..1902)
                     /locus_tag="ACHK01_003518"
     tRNA            complement(1815..1902)
                     /locus_tag="ACHK01_003518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1866..1868),aa:Ser,seq:tga)
     gene            2329..3447
                     /gene="hyaA"
                     /locus_tag="ACHK01_003519"
     CDS             2329..3447
                     /gene="hyaA"
                     /locus_tag="ACHK01_003519"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO8098418.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            3444..5237
                     /gene="hyaB"
                     /locus_tag="ACHK01_003520"
     CDS             3444..5237
                     /gene="hyaB"
                     /locus_tag="ACHK01_003520"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO8098419.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            5256..5963
                     /gene="hyaC"
                     /locus_tag="ACHK01_003521"
     CDS             5256..5963
                     /gene="hyaC"
                     /locus_tag="ACHK01_003521"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO8098420.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLLLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5960..6547
                     /gene="hyaD"
                     /locus_tag="ACHK01_003522"
     CDS             5960..6547
                     /gene="hyaD"
                     /locus_tag="ACHK01_003522"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO8098421.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            6544..6942
                     /gene="hyaE"
                     /locus_tag="ACHK01_003523"
     CDS             6544..6942
                     /gene="hyaE"
                     /locus_tag="ACHK01_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO8098422.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMISELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6939..7796
                     /gene="hyaF"
                     /locus_tag="ACHK01_003524"
     CDS             6939..7796
                     /gene="hyaF"
                     /locus_tag="ACHK01_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO8098423.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQV
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7930..9474
                     /gene="appC"
                     /locus_tag="ACHK01_003525"
     CDS             7930..9474
                     /gene="appC"
                     /locus_tag="ACHK01_003525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO8098424.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            9486..10622
                     /gene="appB"
                     /locus_tag="ACHK01_003526"
     CDS             9486..10622
                     /gene="appB"
                     /locus_tag="ACHK01_003526"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8098425.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10635..10727
                     /gene="cbdX"
                     /locus_tag="ACHK01_003527"
     CDS             10635..10727
                     /gene="cbdX"
                     /locus_tag="ACHK01_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO8098426.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10803..12101
                     /gene="appA"
                     /locus_tag="ACHK01_003528"
     CDS             10803..12101
                     /gene="appA"
                     /locus_tag="ACHK01_003528"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO8098427.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSESELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSKLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(12221..14401)
                     /gene="etk"
                     /locus_tag="ACHK01_003529"
     CDS             complement(12221..14401)
                     /gene="etk"
                     /locus_tag="ACHK01_003529"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO8098428.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLDWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(14421..14867)
                     /gene="etp"
                     /locus_tag="ACHK01_003530"
                     /gene_synonym="yccY"
     CDS             complement(14421..14867)
                     /gene="etp"
                     /locus_tag="ACHK01_003530"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO8098429.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14855..15994)
                     /gene="gfcE"
                     /locus_tag="ACHK01_003531"
     CDS             complement(14855..15994)
                     /gene="gfcE"
                     /locus_tag="ACHK01_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO8098430.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTANADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKIFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(16040..18136)
                     /gene="gfcD"
                     /locus_tag="ACHK01_003532"
     CDS             complement(16040..18136)
                     /gene="gfcD"
                     /locus_tag="ACHK01_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8098431.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(18136..18882)
                     /gene="gfcC"
                     /locus_tag="ACHK01_003533"
     CDS             complement(18136..18882)
                     /gene="gfcC"
                     /locus_tag="ACHK01_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8098432.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLHITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18879..19523)
                     /gene="gfcB"
                     /locus_tag="ACHK01_003534"
     CDS             complement(18879..19523)
                     /gene="gfcB"
                     /locus_tag="ACHK01_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO8098433.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19630..19935)
                     /locus_tag="ACHK01_003535"
     CDS             complement(19630..19935)
                     /locus_tag="ACHK01_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098434.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         4695 a         5103 c         5363 g         4954 t
ORIGIN      
        1 aaacattatt gagacaaaca gccatggctg gttcccggat acagatggtg cgctcatcac
       61 cggactgacc tttcttgacc ccaaagatgc cacacgggtt cagggttttt ttcagcattt
      121 gcaggtcagg tttggtgacg ggccgtggca ggatgttaag gggcttgatg aagtgggcag
      181 tgatacaggc agaacaggag aatgacatga atatactaaa aaaacttatg cagcgtctgt
      241 gtggttgcgg aaagcatgat ggccgtgaac acgtgcagtc gcttacagca cagctgcgac
      301 tgggaccggc agacattctg gagtccgatg agaatggcat tatcccggag caggccaggg
      361 taatcacgca ggtggtgata ctggatgcgg ataaaaagca gatacagtgt gtggtaagac
      421 cgctgcaaat cctgcgtgct gacgggaggt gggaaaatat tggcggaatg aaatagccga
      481 cagcttcaca aaaaccggag tccggctccg gtttttgttg gttagatgta atctgacaga
      541 tacctgtata aataaccggt aactgtcagg tcagagctaa tacaggtaat tatattataa
      601 tcacgagcgc ttagatgtat taataccatg ctctgcaaga tgctgcatca gacgctgagc
      661 cacatcaggc agaggtcttg attgttcatt ttgtaccggc ggcgttggcg caggccagtt
      721 aggtgccgga ggaatatatt cagccatatt ccgggctggt tgaatgccat gctctgcaag
      781 atgctgcatc agacgctgag ccacatcagg cagaggtctt gattgttcat tttgtactgg
      841 cggtggtggc gcaggccagt taggtgccgg aggaatatgt tcagccatat tccgggctgg
      901 ttgaatgcca tgctctgcaa gatgctgcac cagacgctga gccacatcag gtagaggccg
      961 atgtaaagcg tttttttcta tacttgactg agtataagat gtgggacgtt gaagtcctga
     1021 ttctgtataa gaatttttta ccttaaaaat agttgttgca ctggcaggac tgttgctggg
     1081 agaaaaaggt gagctggttt ttaccgtgtt aggtgtgctt ttgtgctgcg agacagtgaa
     1141 ggtactgctg cgtatagcac ttgatatact gcgaggaagg ctggaaaaaa aagaattaat
     1201 gctatttatc atatagataa ccttataaga gctttattgg atagtcttat ttatccgttt
     1261 acatgaaatc taccttttgt gaaattctcg tcaatatttt actcaaaatg atcggctgta
     1321 ggaataaact atttgatatt attacgtttt tagggttgtt ttcaatcagc tatagatagg
     1381 gcttggcgcc agaatggaga atatttaacg aggaatagaa gctttgtatc agacgggctt
     1441 ccctaaatag agaagttaaa taatcaggat gatgctaatg aatatcaggg acaaggtgag
     1501 tgtcttcatg ttcagtgaaa cagggtgtca caaaaaagat tggggggggc tagagccttc
     1561 tgcacgaaaa tagtgatgcg acagatctga gatagctttg tatccttctg cctggttcag
     1621 gcctagagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1681 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1741 ccaattggta taccaagttt ataccaagag ccaaaaaagc aaaggggcta ccatttggta
     1801 accccttgat tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
     1861 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
     1921 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
     1981 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
     2041 ttttactcgt ttagcaactg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
     2101 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aaagtttcac
     2161 tacgctttat taacaatact ttctggcgac gtgcgccagt gcagaaggat gagctttcgt
     2221 tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc ttgccgacca
     2281 taagcgcccg gtgtcctgcc ggtgtcgcaa ggagaagaga cgtgcgatat gaataacgag
     2341 gaaacatttt accaggccat gcggcgtcag ggcgttaccc gacgcagctt tctcaaatat
     2401 tgcagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg
     2461 gcgctggaga acaaaccgcg cattccggtg gtatggatcc acggtctgga atgcacctgc
     2521 tgtaccgaat cttttatccg ctccgctcac ccactggcaa aggacgtcat cctttccctg
     2581 atttccctcg attacgacga tactttgatg gctgccgccg gaacccaggc ggaagaagtc
     2641 tttgaagaca tcatcgcgca atacaatggc aaatatatcc tcgcagtaga aggtaatccg
     2701 ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat tgagaaactc
     2761 aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc
     2821 tgcgtgcagg ccgcgcgacc taatccgacg caggcaacgc ctatcgacaa agtcatcacc
     2881 gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat gagcgccatc
     2941 attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg ccgtccattg
     3001 atgttctatg gtcagcgaat ccacgataaa tgctatcgcc gcgcccactt cgacgccgga
     3061 gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgtct gtacaaaatg
     3121 ggctgcaaag ggcctaccac ctataacgcc tgttcctcca cacgctggaa tgatggcgtt
     3181 tctttcccaa tccagtctgg tcacggctgc ctgggttgtg cggaaaatgg tttctgggat
     3241 cgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca ttccaccgcc
     3301 gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc
     3361 gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac tgaacatcag
     3421 ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga tacaccatca
     3481 ataatgccgg acgccgcctg gtggtcgacc cgattacgcg cattgaaggc cacatgcgct
     3541 gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt
     3601 ttcgcgggct ggagatcatc ctgcaagggc gcgacccgcg cgatgcgtgg gcgttcgttg
     3661 aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac gccatcgaag
     3721 atgccatcgg tattaaagtg ccggacaacg ccaatatcat ccgcaacatt atgctggcaa
     3781 cgctctggtg ccacgatcat ctggtgcact tctatcagct cgccgggatg gactggatcg
     3841 atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg caaagtctct
     3901 cctcatggcc gaaatcatcc cctggctatt tcttcgacgt acaaaaccgc ctgaaaaaat
     3961 ttgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac ccgcagtaca
     4021 aactgccgcc agaagccaac ctgatgggct ttgcccacta tctagaagct ctcgatttcc
     4081 agcgtgaaat tgtcaaaatc cacgcggtct ttggcggtaa aaacccgcat ccaaactgga
     4141 ttgtcggcgg gatgccttgc gccattaaca ttgacgaaag cggcgcggtc ggggcggtca
     4201 atatggaacg cctgaacctg gtgcagtcga ttatcacccg cacggcagac ttcattaaca
     4261 acgtgatgat ccccgacgcc ttagccatcg gtcagtttaa caaaccgtgg agcgaaatcg
     4321 gcacgggtct ttctgataaa tgtgtcctca gctacggcgc attcccggat attgccaacg
     4381 actttggtga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc gacttcaaca
     4441 atgtgctgcc agtggatttg gttgatccgc agcaggtgca ggagtttgtc gaccacgcct
     4501 ggtatcgtta tcccaacgat caggtcgggc gtcatccatt cgatggcatt accgacccgt
     4561 ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg aatgaacagg
     4621 aacgctactc gtggatcaaa gcgccgcgct ggcgcggtaa cgcgatggaa gtggggccgc
     4681 tggcacgcac cttaatcgct tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc
     4741 gcatgatgtc ggcgctgaac ctgccgcttt ccggtatcca gtcaacgttg ggccgcattt
     4801 tgtgccgcgc gcacgaagca cagtgggccg caggtaagtt gcagtatttc ttcaacaagc
     4861 tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc cacggaaaaa tgggaacctg
     4921 caacttggcc gacagagtgc cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag
     4981 gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca
     5041 cctggaacgc cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa gcagcgctga
     5101 tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact ctgcacagct
     5161 ttgatccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc gagctgatct
     5221 ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc gacaacgttg
     5281 tcagccacta tgtctttgaa gcgccagtgc gtatctggca ctggctgacg gtgttatgca
     5341 tggcggtgtt gatggtcacc ggttacttta tcggcaagcc gctaccttcc gtcagcggcg
     5401 aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc gccgggatga
     5461 tttttaccgt ggtcttgctg atgcggatct actgggcttt tgttggcaat cgatactccc
     5521 gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa
     5581 tccgctggta tctgcttctg gcaaaacgtc cgagtgccga tataggccat aaccccatcg
     5641 cccaggcggc gatgttcggc tatttcctga tgtcggtctt tatgatcatc actggctttg
     5701 cgctgtacag cgaacacagc cagtacgcta tttttgcgcc gttccgttat gtggtggaat
     5761 ttttctactg gacgggcggc aactcaatgg acattcacag ctggcatcgg ctggggatgt
     5821 ggctgattgg cgcatttgtg atcggtcatg tctacatggc gctgcgtgaa gacatcatgt
     5881 ccgacgacac ggtgatctcc actatggtca acggctaccg tagccacaaa tttggcaaaa
     5941 taagtaacaa ggagcgttca tgagcgagca acgcgtggtg gtaatggggc tgggcaacct
     6001 gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa cggctgtatg cccattacca
     6061 ctggcccgag tatgtggaga ttgtcgatgg cggtactcag ggactgaact tgctggggta
     6121 tgtcgaaagc gccagccatc tgttgattct cgatgccatt gactacgggc tggaacctgg
     6181 aacgctgcga acctatgccg gagaacgcat tccggcttat ctcagcgcga agaaaatgag
     6241 cctgcaccag aacagtttct ccgaagtgct ggcgctggca gatatccgcg gacatctgcc
     6301 agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc gacgactacg gcggcagcct
     6361 gagcgaactg gcgcgggagc aactgcccgc tgcggaacaa tcggcgctgg cgcaactggc
     6421 tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct
     6481 gtcgatggaa aattacgaag gcgttcgctt gcgccagtac cggatgacac aggaggagca
     6541 gggatgagca acgacacgcc atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg
     6601 acgccggtca gtgaatcccg tcttgacgac tggcttacgc aagcgccaga cggcgtggtg
     6661 ttattaagca gtgacccgaa acgtacgcca gaggtcagcg ataatccggt aatgattagc
     6721 gaattactgc gcgagtttcc cgactataca tggcaggtgg cgattgctga ccttgagcag
     6781 agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc ccgccacttt agtgtttacc
     6841 ggcggaaact atcgcggtgt gctgaatggt attcacccgt gggcggaact gataaacctg
     6901 atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat gagcgaaact tttttccatc
     6961 tgctggggcc aggaacgcaa ccgaacgatg acagtttcag catgaatcca ctgccgatca
     7021 cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga gcaatgtgct cacagcccgc
     7081 aggtgattgc gctgttaaac gagttacaac atcaactaag cgaacgccag ccgccgttgg
     7141 gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga cgatcgtcac tttatcaata
     7201 cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca ggttgacgac agtgaaagtg
     7261 aaatacagga ggcgatcttc tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt
     7321 tgctggagga caaactggag gctggctgcg cgccgctggc gttgtggcag gcggcaacgc
     7381 aaaatctctt gccgacagat tcgctgttac cgccgcccat tgatggcctg atgaatggcc
     7441 taccgttggc gcatgagtta ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca
     7501 ttaatctgac gcaattaccc atcagcgagg ctgatcggct ttttctctca cgtctctgtg
     7561 ggccgggaaa tattcagatt cgtaccattg gctatggcga gagctatatc aacgccacgg
     7621 ggttacgcca tgtctggcat ttacgctgta cggacacctt aaaaggcccg ttactggaaa
     7681 gttatgaaat ctgcccaata ccggaagtgg tgctggcagc gccagaagat ttggtcgact
     7741 ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag
     7801 acggtaagta tcgctttcag gcttatgaat atcgcaatcg gcgaatacct ctggtcgtag
     7861 agtttcagga taaagaggga gatctaccat tatcgggtta tttttctctc ttcgcctaca
     7921 ggagtgcgca tgtgggatgt cattgattta tcgcgctggc agtttgctct gaccgcgctg
     7981 tatcactttt tatttgtacc ccttaccctg gggctgattt ttttgctggc tatcatggaa
     8041 accatttacg tggtcaccgg caaaacgatc taccgcgata tgacgcgctt ctggggtaag
     8101 ctcttcggta tcaactttgc tcttggcgtg gctaccggcc tgacgatgga gtttcagttt
     8161 ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc accactggcg
     8221 atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt cttcttcggc
     8281 tggcaacggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc gttcggttca
     8341 aatctctctg cgttgtggat attgaatgcc aacggttgga tgcaataccc gaccggtgcg
     8401 cattttgata tcgacaccct gcgtatggag atgaccagtt tcagcgagtt ggtctttaac
     8461 ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac cggggccatg
     8521 tttattatgg cgatcagcgc ctggtattta ctgcgcggac gggagcgcga tgtcgcatta
     8581 cgctcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac cctgcaactc
     8641 ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa
     8701 ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg gccggaacag
     8761 gatcaagagc gtaacgcctt tgctatcaaa attcccgcgc tgctagggat cctcgccact
     8821 cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac ctacccacgc
     8881 ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag
     8941 ccgcatgtgt tgcaggcatt ccgggagctg gaaggcgacc ttggttacgg catgttgctc
     9001 tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt
     9061 ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt gggctgtggt
     9121 tccctgctgc tactggtgat gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac
     9181 cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg gattgcgatt
     9241 gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca ggacatctta
     9301 ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc actgatcatg
     9361 atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca gaaatatgcc
     9421 cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga
     9481 aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt ggcgtgatcc
     9541 tggtggtctt tatgatctcc gacggatttg acatggggat cggctgtttg ctgccgctgg
     9601 tggcgcgtaa tgatgatgaa cgccggatag tgattaatag cgttggtgcg cactgggaag
     9661 gcaatcaggt ctggttgatc ctcgcaggtg gagcattatt tgccgcctgg cccagagtgt
     9721 atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc tcactgttct
     9781 tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt
     9841 gggatgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt atcgccttcg
     9901 gcaacttgct gctcggcgtg ccgtttgcct tcacaccgca attacgcgtg gagtatctcg
     9961 gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattactc agccttggga
    10021 tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg attcatctgc
    10081 gttcacagct ggcgactaaa cgcgccgcac tactggtgat gctgtgcttt ttgctggcgg
    10141 gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat gctaacggtc
    10201 cttccaatcc gttaatgaaa ctggtagcag tgctacctgg tgcctggatg aataatttcg
    10261 tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca ttgctgacgg
    10321 tgatggcgat ttatcgtggt cgcccgggtt ggggattttt aatggcgtca ttgatgcaat
    10381 ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga
    10441 gtccaatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg ctgagcatta
    10501 tgttggtaat cgtgctgata tttttgccca ttgtgttgct ctacactctc tggagctact
    10561 acaaaatgtg gggacgcatg acgacagaaa ctctccgccg taacgaaaac gagctgtact
    10621 aaggagcagg gacaatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    10681 tctccaccct tgtgttggta tggctggacc cgcgattgaa aagttaacga acgtaagcct
    10741 gatgcggcgc attagcgtcg catcaggcaa taatgtcgga tatgaaaagc ggaaacatat
    10801 cgatgaaagc gatcttaatc ccatttttat ctcttttgat tccgttaacc ccgcaatctg
    10861 cattcgctca gagtgagtcg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg
    10921 gtgtgcgtgc cccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc
    10981 caacctggcc ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcttatc
    11041 tcggacatta ccaacgccag cgtctggtgg ctgacggatt gctggcgaaa aagggctgcc
    11101 cgcagcctgg tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg
    11161 aagccttcgc cgccgggctg gcacctgact gtgcaataac cgtacatacc caggcagata
    11221 cgtccagtcc cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg
    11281 cgaacgtgac tgacgcgatc ctcagcaggg caggagggtc aattgctgac tttaccgggc
    11341 atcggcaaac ggcgtttcgc gaactggaac gggtgcttaa ttttccgcaa tcaaaattgt
    11401 gccttaaccg tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac
    11461 tcaaggtgag cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga
    11521 cggagatatt tctcctgcaa caggcacaag gaatgccgga gccggggtgg ggaaggatca
    11581 ccgattcaca ccagtggaac accttgctaa gcctgcataa cgcgcagttt tatttgctgc
    11641 aacgcacacc agaggttgcc cgcagtcgcg ccaccccgtt gttggatttg atcatgacag
    11701 cgttgacgcc ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtgc
    11761 tgtttattgc cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact
    11821 ggacgcttcc aggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct
    11881 ggcgtcggct aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac
    11941 agcagatgcg tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga
    12001 ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg ttcgttggcc ggttttacgc
    12061 aaatcgtgaa tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc
    12121 agttacctga atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga
    12181 aaaggcgttc acgccgcatc cggtcacttt accttcagtt ttactctttc tcggagtaac
    12241 tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat
    12301 tgaggattgc gcctttaata ttaacgccag cctgttccag acgctgcatt gacaagctca
    12361 cctctttggc ggtgttcagg ccaaaacgcg caaccagcag gctggtgccg acagagcgtc
    12421 ccacgaccgc agcatcgctc accgccagca tcggcggcgt atcgacaatc accagatcgt
    12481 aatggtcgtt cgcccaatcc agtaattgac gcatccgatc gcgcatcagc agttcagacg
    12541 ggttaggtgg cacctgaccg cgagtaatca catcaaagcc tcctttgcca aaatgctgga
    12601 tcactttgtt gagctcatct ttacctgcca gatattccga caagccatgt tcattactca
    12661 ctgtaaacag gttatgcgaa taaccgcggc gtaagtcggc atcaataaat aacacttttt
    12721 gatcggactg ggcgatcacc gctgccagag ttgaactgac aaacgtttta ccactgtctg
    12781 gcgtcgcacc ggtgatcatc agaatgttat tctccgtctc catcatagcg aagtgcagac
    12841 tggttcgtag cgcacgtacg gcttccacag cagaatccgc cgggttatcc acagccagga
    12901 aggggatatt tttagtacga tggcgctgct gattagaaaa taaatttttc ttacgcagac
    12961 gggtacgttt atccagccac tcggacattg ggatagtggc ataaacgctg atgccgtgct
    13021 cttccagttg ttccggggct tctacaccac gacgcaacat cgcacgcgcc agcacggcac
    13081 ccaccgaaat aaacaggcca agaataaaac caagcaccac attcaacgct tttttcggtt
    13141 tcactggctg cggctgagtg actgccgggt cgataatccg cacgttacca atggcactgg
    13201 atttcgaaat actcaactcc tgctggcggt taagtaattg cagatatacc gcacggcccg
    13261 cttctacgtc acgacttaaa cgcaacactt cctgttgggt ggaaggcata gccgataccc
    13321 gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc agcgcacgat
    13381 aagttgggtg atctttctta tacagctggg agatctctgc ctcgcggaaa gtcagctcat
    13441 tgagttgatt atcaacgttc acaatctgct caagaacggc tttggcttcc aggttaaggt
    13501 caaccgaatc gcgctgctgg cgataaacgt tgagtttttc ttccgcctgg tccagctcgc
    13561 tgcgcacttc aggcaactgg cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct
    13621 ggcgagcgat attctgttgc aaatagttgt tagcgatgct gttcagaata cgagtaatca
    13681 actggggatc atcaccggtc atggtaagtt ccagcatccc gctttcttta ctgcgttcgc
    13741 taacggtaaa ggtttcctgc aacgcgttaa tcgcttccag ttcggtacgt tggctcagga
    13801 caaactgtgt tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg ccatcttttt
    13861 ccagacgctg gccgaccata ccattgacgg tgaactcttc accttccagc gtatagtggc
    13921 cgttttcccc aaccgtgagt gtcagttgct gatcctgacc attcagttgt ggaatatgca
    13981 tccagctgat cgccagctca cctggtttct ctttggttaa tctcgcccag ccgcgaccca
    14041 caatcggaaa atacttttgc tcaaccatgt cacgcagatt cagttcagca atggttttac
    14101 cgagaatcat gcgcgattgc agcagttgga tctccggtgc agactcgggc gatgagttag
    14161 ggatcatatc gctcaggccg ctgagaatgg cgttgccctg tttttgctca acctggacca
    14221 gagtatctgc ctgataaatt ggtgtgctta acagcgagta agcgacagcg atcagcgtga
    14281 ataacgcggt cacgctgata ataaacttac ggtgatccca taactcgccg accagacgaa
    14341 gcagatcgat ctcattttcc tgagtgctgc ctggtggcgt attcatattt ttagttgtca
    14401 tcgtatttta taaactcaaa ttaccggctg aggcgcttcg cccattcctg actggcgcgc
    14461 tccaacatac cgtagacatg ttcaaatgcg tcctgacttt tacgataggg atccgggatc
    14521 tctttctgtt ccagccattg cccaaacagc attgtcttcc cgcggacctc gggagcgatt
    14581 gcggtaacct gagcaatatg ttccgactcc attgccagaa tcagatcgta atttcgtgcc
    14641 atctcagcag tgagcttacg tccggcatgt ccttcaagag atacgccatg attagcggca
    14701 acatctgccg ctgtcgcatc tgcagggtgt tttaccagac catgaacacc tgccgatttg
    14761 acttttacgc ccggcagacg tttacgcagc aagcgctcgc caatgggcga acggcaaatg
    14821 ttgccggtac aaaccaccag gattgagtta aattttagtt gggccatgtt tgaatccacc
    14881 gtgaagtctc tgtcaggtca tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac
    14941 ggttccaacg agccagcggc gcggtagtga cataaacaat gtcgtaaggt tcaagctgga
    15001 attccgttcc gagtatcatt gccgatgcgt ccttcgcatt cagctgataa atattggcga
    15061 tttttccatt ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc gcatcagcga
    15121 catcctgatt cataccctca gcgttaccca gcgcttccgc cagggtcata ccgctgcggt
    15181 ccattttcag cgtgctctgt ttacccactt cacccatcac gaagattttc agatcatcgt
    15241 tgcgcggaat aaataaaata tcgcccgggt gcagcagttt gttctgacgc aagtcgccac
    15301 gctgcatcag cgcatagagg ttcactttgg tctttacgcc attctgggtg agcaccacat
    15361 tgcgccagtc tgcatttgcg gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca
    15421 gaggaatatt ggtaatcggc tgctgacccg atttgctgac ttcgccggtg acgtacgctt
    15481 tttgcgatcg gaacgctgcg acgctgacat cgacctgcgg gctttcaatg actgaatcga
    15541 gacgcgctgt aatctcgttg cgtacctggg taagtgtttt cccggccact ttcaggcgac
    15601 caatataggg gtagaaaatc gcaccatcgg cgttaaccca gttgccggta tcactggcgc
    15661 tacggtattg ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta accatcaaca
    15721 catcaccaat gccgatacga tattcatagt tcgcgatttg ctgctccagc tccgggttag
    15781 cctgcgacat aatcggtttg gcgcgcagct gatcaatcaa tcctggcgtg acgggataga
    15841 tattgaccat tttgtccagg tcatactgat tgtcaggtag ttcaatgaca tccttgttac
    15901 tggtcgataa attctgacca ggaacaaggg tacatgctgt caaagacaga acagccaatg
    15961 tcagtaccga aaacttaaaa atgttctttt tcattggttt atatttatca gtcaatgtaa
    16021 tgttatgaat tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc gtcattggat
    16081 agagctggta tttgcgtgac agcatttgtc caccatcacg cgtcaatggt gtccacgaaa
    16141 ccaccgcgcg gttgcggtta ggtccaatgg tcatcaagtc gaacggaatc gagatataaa
    16201 aacctttact aaagccgcct tcgccgtagt catctttaga tacgttactg attgccgccc
    16261 atacccctac cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt gcccctttat
    16321 cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt
    16381 tccagtaagc cgtcacgaaa cctgttggcg tggaataatc ggtgaagcgc atcatgttgt
    16441 cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagctg gcatctagcg
    16501 ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa ccgccataca
    16561 cctggccata gaaaccattg cctaagtcgg caaagtagtt cgcctgcaag ttgttgagat
    16621 aaacgtcatt gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg gtagagtccg
    16681 cgggtaatag cgaagactta aacttgtcgt agttattgta aatattggtg aaaataccgc
    16741 catcaagcag caggtggtca gtaaaccagt agcgggcgct ggacatcagc cccaactgga
    16801 acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc
    16861 taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct tcagcttcaa
    16921 cacgttgttg ttgcagtggc tctgattgac caggcgctgt accggccagc tgtttgcgca
    16981 ggctggctac gtcggtttcg gtagtcacca tagccatatg ctcgcgcttt tgcgtcacgc
    17041 taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg
    17101 cttcacgaga gtcacggtat ttgtattgct gaccagacat atacagcgtc ttatcgcgta
    17161 gctgaatttc tggcgcgtca aagcccgcgt tatacttcag ggcggtaagt tggtttgcta
    17221 ccgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat
    17281 cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa agtgaaacca aacatcaacg
    17341 tgttaccgcg ttcataactc aggttgaggt ctgcccagct ggcagcgcga taaactgcac
    17401 cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc
    17461 cgtcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa
    17521 agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgagact
    17581 ctgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat
    17641 taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg
    17701 ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcta
    17761 cttgcggtag ccagtaacct tcttcccata ggcgaagttt aaaatcgaaa gatttgtctt
    17821 tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg cgcacatccg
    17881 tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaacggt
    17941 attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg
    18001 gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc ggatccgggt
    18061 aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat
    18121 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
    18181 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
    18241 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
    18301 ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg
    18361 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccagtcc cagaaaccgc
    18421 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccag
    18481 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
    18541 accagtgata tggagattga gtaactgctg gcgcacggat ttaatcgttg ctgccacatc
    18601 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
    18661 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
    18721 cagttgcggt tgtgtcacca actggacaac attttccacg gggccaacgg aaagtgtctg
    18781 ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
    18841 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
    18901 gtcatttcta cgggaataac ccctgcgcct aacatttgtc gactttgacg aacctggcca
    18961 gtagccgaat cgacccagaa tatgttttgc cactgttttt ctggcagcgt ggaagtcacc
    19021 tcttcctgcc agacattaca cagcacatcg cgacccgcaa tattcagcgt ctctaatcca
    19081 gcaaaggaaa aagtggaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga
    19141 atatcgcgcg tccagcggct gccgtctttt attgccagcg cgttacgcag agggtcctgc
    19201 ccggaatttg tcacttccag cagattatta ttaagtttga cggtctttaa aagacgtcca
    19261 ttatgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
    19321 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat cgtggaataa
    19381 ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg
    19441 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
    19501 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
    19561 ctctaaagcc cggtacgaat atcgcaaccg ggtattattt aactttaatt tcgacgcgtt
    19621 taagcagaat tagcgcgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
    19681 actgtcattt gctgcaacta ctgccaacgt cgccacacca acagccgttg ccacaccgac
    19741 aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagcag ccgtcgtggt
    19801 tgcaccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc
    19861 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
    19921 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat
    19981 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgcttg
    20041 cactatatac agcgggtgag aaaggcgcgc agagggaaat ataagattgt taacgatttc
    20101 cctctggaaa tacga
//
LOCUS       DAZGCS010000090        19593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGCS010000090 DAZGCS010000000
VERSION     DAZGCS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.090"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            255..755
                     /gene="tssB"
                     /locus_tag="ACHK01_003536"
     CDS             255..755
                     /gene="tssB"
                     /locus_tag="ACHK01_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8098435.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            790..1014
                     /locus_tag="ACHK01_003537"
     CDS             790..1014
                     /locus_tag="ACHK01_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098436.1"
                     /translation="MTAHKNISETYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1065..2540
                     /gene="tssC"
                     /locus_tag="ACHK01_003538"
     CDS             1065..2540
                     /gene="tssC"
                     /locus_tag="ACHK01_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8098437.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPWSPADNPVKSFNYHEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2547..2960
                     /gene="tssE"
                     /locus_tag="ACHK01_003539"
     CDS             2547..2960
                     /gene="tssE"
                     /locus_tag="ACHK01_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8098438.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2964..4814
                     /gene="tssF"
                     /locus_tag="ACHK01_003540"
     CDS             2964..4814
                     /gene="tssF"
                     /locus_tag="ACHK01_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8098439.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4778..5860
                     /gene="tssG"
                     /locus_tag="ACHK01_003541"
     CDS             4778..5860
                     /gene="tssG"
                     /locus_tag="ACHK01_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8098440.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPELSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5885..7165
                     /gene="tagH"
                     /locus_tag="ACHK01_003542"
     CDS             5885..7165
                     /gene="tagH"
                     /locus_tag="ACHK01_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO8098441.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7162..7686
                     /gene="tssJ"
                     /locus_tag="ACHK01_003543"
     CDS             7162..7686
                     /gene="tssJ"
                     /locus_tag="ACHK01_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8098442.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7689..9020
                     /gene="tssK"
                     /locus_tag="ACHK01_003544"
     CDS             7689..9020
                     /gene="tssK"
                     /locus_tag="ACHK01_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8098443.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRAIMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9025..9786
                     /gene="icmH"
                     /locus_tag="ACHK01_003545"
     CDS             9025..9786
                     /gene="icmH"
                     /locus_tag="ACHK01_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO8098444.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYIPCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9795..12550
                     /gene="tssH"
                     /locus_tag="ACHK01_003546"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9795..12550
                     /gene="tssH"
                     /locus_tag="ACHK01_003546"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12547..13290
                     /gene="vasI"
                     /locus_tag="ACHK01_003547"
     CDS             12547..13290
                     /gene="vasI"
                     /locus_tag="ACHK01_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO8098445.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13295..14707
                     /gene="tssA"
                     /locus_tag="ACHK01_003548"
     CDS             13295..14707
                     /gene="tssA"
                     /locus_tag="ACHK01_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8098446.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14816..18250
                     /gene="tssM"
                     /locus_tag="ACHK01_003549"
     CDS             14816..18250
                     /gene="tssM"
                     /locus_tag="ACHK01_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO8098447.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGSRGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHTLGWLKEKRARQPLNGIILTLDLPDLLTADKRRREYLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRDWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGKDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWMPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18261..>19593
                     /locus_tag="ACHK01_003550"
     CDS             18261..>19593
                     /locus_tag="ACHK01_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO8098448.1"
                     /translation="MDSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAAT"
BASE COUNT         4555 a         5418 c         5472 g         4148 t
ORIGIN      
        1 tatttaaatt attgccataa tacacctgat gcgaattgca caggttcaca caatctattt
       61 aacgcttgca gaatacccct ctttacgatc gataaaaagt ccttttctta tttattttga
      121 agcgtaaaat tcggggtgat gaaggagtgg tgattttatt ttcaccgcca ttagggcggc
      181 attgccgata tttttttgcg gtacaaatat gccgtgttca tggcctgtac gagataacat
      241 gacggatcac ttttatgagc aaaaaatttg aaggcagcgt cgcgcctcgc gagcgtatta
      301 atatatcgta tgtcccaaaa acagatggcc agacggctga agtggaactt ccgcttaaca
      361 tgctcgttgt cggcgatacc ggaaatacgc aggaaacatc cccactggat gaacgtcagg
      421 cagtttccgt taacaagcac aatttcggtg cagtgatggc tgaagcagcc attggcctca
      481 attttactgt ccccgccacg ctgaagggaa gcacaacaga cgatgaactg aatgtggcgc
      541 tgaatatcaa atcactggat gatttttcac cagatagcgt ggcccggcag gttccggaag
      601 tgaataaatt gctggaactg cgtgaagcac tgactgcact gaaggggccg atgggtaacc
      661 ttccggcatt ccgtacccag ctccaggcat tgctggaaaa tgaagagtcc cgcgagcagt
      721 tgctcaaaga aatcggtctg gtgagcaata aataaccacc actatttcat taaacagggt
      781 tatccaggca tgacggcaca caaaaatatc tccgagacat accacctctc tgttgcagac
      841 attctgcagg tggtttacca ggtatgtttc agcccgtccg ttgaaatcaa ccaggatggt
      901 gtggctgcgc tgattacaac actggatcgc cgtatcagtg atctattgga tgagattatc
      961 catttttgtg agtttcagca gagcgcgtcg cactggcagc gcgtacttca ctgatggcga
     1021 aaagccggtt tcagaaacac agctaaaaca gaaggatttt cagtatgtct ttacaggaag
     1081 aggaactcgt ttccagtcat gccgggcagc ctgagcaggc atcgtcgctg cttgatcaaa
     1141 tcatggcaca gacccgtatt cagcccggtt cggaaggtta tgacgttgcc cgccagggtg
     1201 tgacagcctt tattgccagc atcctgcaat ccactgcatc tgccgaaccg gtcaataaac
     1261 tggccgtgga cagcatgatt gcagatattg atgaacgcat cagccgccag atggatgtca
     1321 tcatccatgc gcctgccttt cagcaggtcg aatcgttctg gcgctcactg aaaaccatgg
     1381 tggatcgcgt tgatttccgt gaaaacatca aggtcaacgt gcttcatgtc accaagcagg
     1441 aacttctgga agactttgag tttgcgccag aaattattca gtccggattt tataagcacg
     1501 tttattcgtc cggattcgga cagtttggtg gtgaacccat cgctgccgtg ctgggcgctt
     1561 atgagttcaa aaataccgca cctgacatga aactgctgca atacgtcagt gccgtgggcg
     1621 caatggcgca cgcaccgttc ctgtcctccg tttccccgga gtttatgggg ctgaactcgt
     1681 ggaccgaact gcctaatatc aaagatcttt atgccatctt tgaaggtccg gcctacacca
     1741 aatggcgcgc cctgcgtgac tcggaagatt cgcgctattt agggcttact gcaccccgct
     1801 tcctgcttcg ccagccctgg tcaccagcag ataaccctgt taaaagcttt aactatcacg
     1861 aagatgtcag ccagaaccac gaagattatc tgtggggtaa tacggcctgg atgctggcat
     1921 gcaatatcgc agacagtttt gccaaatacc gctggtgtcc aaacattatc ggcccgcaaa
     1981 gcggcggcgc ggtgaaagat ctgccggtgc atctgttcga aacgatgggg cagattcagg
     2041 ccaaaatccc aaccgaggtg ctggtcaccg atcgccgcga atttgaactg gctgaagagg
     2101 gttttatcac cctgaccatg cgtaaagact ctgataatgc agcctttttc tctgcaaact
     2161 cggtacaaaa accgaagcac tttccgggaa aagatgcaga aaccaactat aagctgggta
     2221 cgcagcttcc ttatctcttc atcatcaacc ggttagcgca ctacatcaag gtgttgcagc
     2281 gtgaacaact ggggtcatgg aaagaaagga gcgacttaga gagggagctt aatacctgga
     2341 tccgtcagta tgttgccgac caggaaaacc cgcctgcgga cgtgcgcagc cgtaaacctc
     2401 tgcgcgcagc cagagttgag gttatggatg tggaaggcga acccggctgg taccaggttg
     2461 cgctaagcgt gaggcctcat tttaaattta tgggggcaaa ttttgagctt tccctggttg
     2521 gccggttaga cagggagtaa cacaccatga taccggatcg ggaaagaacc acaggttctc
     2581 tttttgagcg tatggaagcc tcttcggcca gaaaccggca gggaggcagt attcattcgc
     2641 tcaggcaatc cattcgccag aacctacgaa acattcttaa tacccggtcc ggtagttgcc
     2701 ggggtgcgcc tgagctgggc attgatgaac cggaaggggc tgaaaacttc cgcgaatcca
     2761 tgagtcgggc catcgagcaa tgtatagaac gctatgaacc ccggatctca cacgctgaag
     2821 tgcaggcagt ggtttcatcc gcctccagcc cgctggatat gacgtttcat attacagcct
     2881 gggtgacatt taacgaaacg catgaggtgc ttgaatttga tatggcaccg aatggcagcc
     2941 agcattaccg ggtggactga caaatggaat ttgaagaacg ctatttccgg gaagaactcg
     3001 attacctgcg ccagcttagc aagctgctgg caacggaaaa accccatctg gcccgcttcc
     3061 tggccgaaaa agatgcggat ccggatattg aacgcctgct ggaaggggtg gcttttctta
     3121 ccggcaatct ccgccagaaa attgaggatg aattcccaga actgacgcac gggcttatta
     3181 agatgctatg gcctaattac ctgcgtccgg ttccggcaat gacccttatt gaatatacgc
     3241 cggatatgga taagtcttct gtaccggtgt taatcccccg taatgagcag tttacaacca
     3301 acgccgggga aatcagagtt gatgaagtgc tgccctctga tgctaaaaag gaggagccgc
     3361 ctccctgtac cttcactctc tgccgggata tctggctgct gcccgttcgc ctggagcaga
     3421 ttgaaaaccg cagtacgacc cgtaatggtg ttatcaacat caccttttcg gtcgcaccgg
     3481 gaacggactt ccgcacgctg gatctgaaca aacttcgctt ctggctcggc aatgacgaca
     3541 actatacccg tgaccagctt tatttatggt tctgcgaata cttgcagggt gccgacctga
     3601 ctgtgggtga acagcatatt cgcctgcctg agtttatgct aaaagctgtc ggttttgagc
     3661 cgcaggatgc catgctgccc tggccgaaaa acgtccacag cggctaccgg atccttcagg
     3721 agtatttctg ttaccccgat gcgtttctct tttttgatct ttgtggttgt ccggctttgc
     3781 ctgacggatt gcaggcggaa ttctttaccc tgcaactgcg tttttcgcgc cctttgcccg
     3841 tggacatccg gctgcgccgc gattccctgc gcctgtattg cgcacctgcc attaatttat
     3901 ttatccacca tgcagaagcc atcacgctgg acaaccggcg ggcagactat ccgctggttc
     3961 ccagccgcca ttacccacaa cattacgatg tattttccgt taacagtgtg gtgagccagg
     4021 tccaggatat gttcaggaaa aaagatctgg ggcgtcctgt ttcgacgcag gccgcgcgcc
     4081 agtggccagc ctttgaaagt ttcagccatc agatggaata cagccggaag cgggaagtgg
     4141 tgtactggca tcaccggacc aaaacatccc tgttccatcg cggctttgat catacccttg
     4201 cctttataca tgctgatggc agttatccgt cagacgaatc tctgctcagt aatgaagtgg
     4261 tttcggtatc gctgacctgt accaaccgtg agcttccgtc acaaatacgt tccggcgata
     4321 tcaccggcac aaccggtaaa aatgcggctg ttgcttcatt tcgcaacatt acccgcccga
     4381 cgcaaccact ctggccggtc attgatggca gcctgcactg gtccctactc tccgccatga
     4441 acctgaatta tctgtcatta ctggatacgg acgcgctgaa gcaggtcatc gccaactttg
     4501 atcgccacgc aatccatcat ccgcagacgg cacggctgtc acaacaaaag ctggatgcca
     4561 ttgagcgtct ggagacccgc cccgttgatc gcctgtttac gggtattccc gtccggggac
     4621 tggcctccac gctgtatctg cacccggagc cgtttgtctg tgaaggggaa atgtatctgc
     4681 tcggtacggt gctttcgcat tttctgtcgc tgtacgccag cgttaactca ttccacatgc
     4741 tgaccgttgt gaacacagaa agccaggaga catggaaatg gacggaaaga atcgggcagc
     4801 atcctcttat ctgagtccgg gaaatccccc ggcagataaa gagcagaatg atccgctggc
     4861 acaggttttc cataatgcct gctcttacaa tttttttgcg atggcggagc tgttgcaccg
     4921 cctggcaaag ggtgaaaagg ggacgccaga attatccctg cgtgacgatc cggcacagga
     4981 aaccctgcgt ttcagcgccg atgccagcct tgccttcccg tgcagtgata tcagtgcact
     5041 gaaacgggat acgtcagggg cattcaggat gaccaccacc tttatggggt tacaggggag
     5101 ccagtcgcct ctccccggtt attacctcga tcacctggcc tggaaagcgg tacatgaaca
     5161 aagtccggta ggtgattttc tggatatgtt cagccaccgc ctgacccagt ttgtctggca
     5221 tatctggcgt aagtaccgct atcacatcag ttttcgtaac ggcggtgtgg atgcgttctc
     5281 gcagcgcatg tactcccttg tcgggctggg ccaccgccag cttcgcgata agctggcgat
     5341 taatcacagc aaaatgctgg cgtattccgg gatcctggca aatccgggac gatccccgga
     5401 aataatctgt ggcctggtgt cccactgctt tgatttgtca gaagtgacgc tgcaaaactg
     5461 gcaacgtcgc aaggtggata ttgaaccgga tcagcaaaat agtctgggga gctattctct
     5521 taaaaatggc gaaaaactgg cgggaagatc ggtactgggc aactttgttc tgggtacacg
     5581 agtgcccgag ctcagcggaa aattccagct cagtattacc agcctgaccc gaaaacagtt
     5641 cctctctttt ctgccgtccg gcgaaaactt cctgccactg acgatgttcg tgtccttcat
     5701 cctgcgcgat cagcttgcct gggacttaca tctggggctg gcgccggagc aggtgggcgc
     5761 aatgcgcctg ggcgataaca aaagtgcgct gctgggctgg accagcttcc tcggcacacc
     5821 ggaagaacga ccctcagtca ccatcagggt acggtcgtaa attatgataa tcacggagca
     5881 gaatatgccg gaagagaaac tacagacgct ttcattgcag gtcattaacg gcagtgagct
     5941 ggaaagcgga cgggcggcgc gctgtctgtt cacacagcag ggaaatgtcg gccatggccc
     6001 cgaatgccac tggtcggtac aggatcgtca gcagagcatt ccggcccagg cttttaccgt
     6061 tatcctgcac gatggcacat tttgtctgcg cccgcagacg gcacaactgt ggctgaatca
     6121 ggcaaaagtc acagcaacat cagacctaat acagttgcgc cagggcgatg agatccagat
     6181 cggacggctg atggtgaggg ttcatctgaa ccggggagat attccccatt acgatgagga
     6241 aatggccact cccgaaacca tcgttaccaa tcgcgatatg ctcacggata ccctgctatc
     6301 aacggagggt acgccacact atccgggaat gactcaccgg caccaacttg cagacaccgt
     6361 ggtaaacggt ttttctgccg atccactcca ggcacttcag tccgaaagcc tgattaccac
     6421 gggcgatccg ctttcaggca ttgcggctgt ccggccatcg gcaccgctgt ccgatccggc
     6481 aagtaatggg gggatcaata ctccgtttat ggatctgccg cccatttatg ccagccccgg
     6541 cgatcgtaat gacgacgtct ctgcggcaga aatggcgcaa cgccaccttg cggtcacccc
     6601 cttactgcgc ggtcttggcg gctcgcttac catgagcaat tccgacgatg cggatgattt
     6661 tctggaggag gccggacgaa cgttacaggc cgcaataaaa ggtctgctcg atttgcagca
     6721 gcagcgtaac agcctctcag acaaacattt gcgcccgctg gaagataacc cgctgcgcct
     6781 gaacatggat tacgccaccg cgctcgacgt aatgtttgcc gaaggtaaaa gcccggtaca
     6841 tctggcggct cccgccgccg tcagtgaaag cctgcgcaat gtccgccacc acgaagaagc
     6901 taaccgggca gcgattgtgg agtcgcttcg tgtcctgctg gatgctttct caccacaaaa
     6961 tctgctgcgc cgctttgtgc agtaccgccg cagccatgaa ctgcgccagc cgctggatga
     7021 tgccggagca tggcaaatgt acagccatta ttacgaagaa ctggcctccg atcgccagca
     7081 ggggtttgag atgctgttta acgaggtcta cgcccaggtc tatgaccggg tgcttcgtga
     7141 aaaacagcgg gagccggaag catgaacagg aaggcgtttc tggcctgcgt gctgatgtgc
     7201 gtcatattaa ccggctgcga aacggcgaaa aaaatcagtg aggtgatcaa aaacccggat
     7261 atccaggtcg ggagcctgaa ggagcaacct tcggaaataa ccgtcaccct gctgaccgaa
     7321 ccggacacca ataccaatgc ggaaggtgaa tcagccgccg tggatgtgca actggtgtac
     7381 ctgactgacg actcaaaatt gctggcagcc gactatgacc agatagccag tacgccgcta
     7441 ccggatgtgc tgggtaaaaa ctatatcgac caccaggatt tcaacctgtt gccggacacc
     7501 atcaaaaccc tgccgccggt aaagctggat gagaaaacgc agtttattgg tgtcgtggcc
     7561 tatttctccg atgaccaggc cactgaatgg aaacaaatcg agacggtgga aggaaccggc
     7621 caccattacc gcctgctggt gcatgtacgt caaagcagca tcgaaatgaa aaaagaggat
     7681 gaataattat ggcaaccacc cgcaacaagg tgatgtggca ggaagggatg ctgatgcgcc
     7741 cacaccattt ccagcaacag cagcgttaca acgactacct ggataaccag cgtttccggg
     7801 ccatgaatga tttatcctgg ggatttaccg aactcaccct caacaatgaa ctgctggcgc
     7861 agggtaagat catgattgac agcgcgtcag gcacactgcc cgacggcacc gtcttttcta
     7921 tccccgacca ggacgcactg cccgatccgc tgcacccgca aaattttccg gacgaaagaa
     7981 gccgcaatat ctacctcgct ctgccggtcg ccagtgatgt gagaaatgaa atcagcgacg
     8041 gacggcgaat cgggcgttac cggctgaatt atgccgatgt ccgggatttg cattcagaag
     8101 aaggtgacgc gcgaacattg acgctgggtc aactgacgcc gcgcattatg agcggtgcag
     8161 aagatatgag cgcctatatt acgctcccgc tttgccgtat cagcgatcgc catgccgatg
     8221 gctccctgac gttggatgac gattttatcc cctcctgcca gaatattcag gtcagtaaga
     8281 aactgcgtgt ttatctcaaa gaggtacagg gggccattgg cggacgggca agcgatctgg
     8341 caaaccgtat tggctctccg gcgcagagcg gcatcgcgga tgtggcggaa tttatgatgt
     8401 tgcagttact taaccgtaac cagacccggt ttacccatcg cgcccgccga tcccagctcc
     8461 acccggaaga tttctacctt gatcttgctg ggttgctggg tgaactgatg acctttaccg
     8521 agccgtcgcg cctgccctgc ccgcttgatg tgtatgatca tcatgatctg accaaaacat
     8581 ttaaaacact gttaccggaa gtcaaacggg cgctacatac cgtactgtcg ccaagagcgg
     8641 tcaatctgcc gctgcatctg cgtgacggta tctggcaggc cgatatccat gacacagaac
     8701 tgctgcaatc tgccaccttt gtgctggccg tggcggcaaa cgtgccggtc gatcagatcc
     8761 agcgtcagtt tatccagcag tcgaaaattt cctcgccgga aaaaatccgc aatatggtca
     8821 gtgtgcagat ccccggtatt ccattgcgtg ccattatggt ggctccccgc cagcttcctt
     8881 atcattccgg gttcagctat ttcgaactcg acaagagcgg acaggcctgg acagaaatgg
     8941 ctgccgccgg ggccgttgca ctgcatgtat ccggcagttt cccggatctg aacatgcaac
     9001 tgtgggcgat aagagggtaa gacgatggat gaggggtcac tgtctctgcc accatttacc
     9061 ggatacgacg aaaaaagcca gcgcaactac catctcgcgc ttcgtggcaa cagcctgaat
     9121 ccgatgatcg acgcggccac gccgctactc ggtatggtaa tgcgcctttc aaccatgaac
     9181 agccagacga tgccggagca tctttttgct caggtggtga cggatgttca ggccgtggag
     9241 caactgcttc aggagcaggg ctacgaaccg ggcgtgatta tctcgtttcg ctacatcccc
     9301 tgcacgttta ttgatgaagc ggcgctcggt aacggctggt caaacaagaa cgagtggatc
     9361 aagcagtcat tgctggtcca tttccacaac gaagcctggg gtggagagaa agttttcatc
     9421 ctgctggagc gcctgatccg tgaacccaaa cgttaccagg atctgctgga atttctctgg
     9481 atctgttttt ctctgggatt tcgcgggcgc tacaaagtgg cagcacagga tcagggggaa
     9541 tttgagcaga tctaccgccg tctgtaccac gttctgcata aactgcgcgg cgatgcccct
     9601 ttcccgctgt tacaccagga caaaaaaacc cagggcgggc gttatcagct catcagtcgc
     9661 ctgactgtta aacatatttt ttgtggtggc gttgtcgtgc tggcgttgtt ttacctgttc
     9721 tatctgttgc ggctggacag ccagacgcag gacatcctgc accagctaaa caaattactt
     9781 cgataaggac gtttatgatc cagattgatc ttcccacgct ggtaaaacgg ctgaacctgt
     9841 tctcccgcca ggcgctggag atggccgcct ctgaatgtat gagtcagcag gcagcggaaa
     9901 ttaccgtcag ccatgtgctc attcagatgc tcaccatgcc acgcagtgac ctgcgggtta
     9961 ttacccgcca gggcgatatt ggcatggaag agttgcgcca ggcgctgacg gtagagaact
    10021 acacaaccgc ccgttctgcg gacagctacc cggcgttttc cccgatgctg gttgagtggc
    10081 tgaaagaggg ctggctgctg gcgtcggctg agatgcagca cagcgaactg cgcggcggcg
    10141 tgttgctgct ggccctgctg cattcgccgc tgcgttatat accgcctgct gccgcccggc
    10201 tgttgaccgt gaccgtctgc aacaggactt tgtgcagtgg acacaggagt cggcggaatc
    10261 agtcgtgccg gatgcagacg gtaaaggcgc aggcacaatg acggacgcct ctgacaccct
    10321 gcttgcccgc tatgccaaaa acatgaccgc agacgcccgt aacggcaggc ttgacccggt
    10381 actgtgccgc gaccacgaaa tcgacctgat gatcgacatt ctctgccgcc gccgtaaaaa
    10441 caacccggtg gtggtgggcg aagcgggcgt gggcaaaagc gcactgattg aagggctggc
    10501 gctgcgcatc gtggcaggcc aggtgccgga caagctgaaa aacaccgata tcatgaccct
    10561 tgacctgggc gcattgcagg ccggggcgtc ggtgaagggt gaattcgaaa aacgtttcaa
    10621 agggctgatg gcggaggtca tttcctcccc ggtgccggtc attctgttta tcgacgaagc
    10681 acataccctg attggcgcgg gcaaccagca gggcgggctg gatatctcca acctgctcaa
    10741 accggcgctg gcgcgcggcg agctgaaaac catcgccgcc accacctgga gcgagtacaa
    10801 aaaatacttc gaaaaagatg ccgccctgtc gcgccgcttc cagttggtga aggtcagcga
    10861 acccaacgct gccgaagcca ccattattct gcgcggtctg tcggcggtct atgaacagtc
    10921 tcacggcgtg ctgattgatg atgacgcctt gcaggccgct gcgacattaa gcgagcgtta
    10981 tctctccggg cgtcagttac cggacaaagc gattgatgtg ctggataccg cctgcgcccg
    11041 tgtggccatc aacctgtcgt cgccgccgaa gcaaatctcg gcgctgacca ctctgagcca
    11101 ccagcaggag gcggaaattc gccagcttga gcgcgagctt cgcatcggac tgcgtaccga
    11161 cacatcacgg atgaccgagg tgctggtgca gtatgatgaa acgctgacgg cgctggatga
    11221 actggaagcg gcctggcacc agcagcagac gctggtccgg gagattattg cgctgcgcca
    11281 gcagttactg ggcgtggcag aggacgatgc ggcgccgttg ccggacgcag ataccgtgga
    11341 ggatacgcag ccagagtcag agtcagaaca ggataatacc ggtgccgtac cggctgatga
    11401 ggccgacaga gaacagccgg aagagaccgc tgaaacagtt tccccggtac aacgtctggc
    11461 acatctcact gccgaactgg acgccctgca taacgaccgg ttgctggttt ccccgcacgt
    11521 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc ttaaccgcct
    11581 gtcgcagaat gaaatgtcgg tcatcaccga cctgccaaaa tggctcggcg acaccatcaa
    11641 aggccaggac ctggcgattg ccagcctgca caaacacctg ctgaccgcac gcgccgacct
    11701 gcgccgtccg ggacgcccgc tgggcgcgtt cctgctggct ggccccagcg gcgtgggtaa
    11761 aactgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    11821 catcaatatg tccgagttcc aggagaaaca taccgtctcg cgactgattg gttcgcctcc
    11881 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    11941 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    12001 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    12061 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    12121 cccggaaacc atgcaggaag tactgtatcc ggtgctggcc gacttcttca aacctgccct
    12181 gctggcgcgt atggaagtgg tgccgtacct gccgctgtcg aaagagacgc tcgccaccat
    12241 cattgccggg aaactggccc gcctggataa cgtgctgcgc agccgctttg gcgcggaggt
    12301 gattatagaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    12361 cgcgaggatg ctggaatcgg tcatcgacgg caatatgctg ccgccgctct ccctgctgct
    12421 gttgcagaaa atggcggcga atacggcggt tgcccggatt cggttgtcgg cagtggacgg
    12481 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    12541 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    12601 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    12661 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    12721 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    12781 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga agcctggacc
    12841 cgcgccacgg aacaggaaaa acaccgccag ggcaacacca cagagctgct ggtgacgcag
    12901 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    12961 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    13021 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    13081 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    13141 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    13201 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    13261 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    13321 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    13381 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    13441 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    13501 gagtaaggat tttcgtctgc tggcgcatct gctgagaacc ctgcaacacg ccggtgatcc
    13561 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    13621 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    13681 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    13741 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    13801 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    13861 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    13921 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    13981 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    14041 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    14101 tggccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    14161 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    14221 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    14281 ggcagacgcc atccgcgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    14341 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    14401 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    14461 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    14521 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    14581 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    14641 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    14701 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    14761 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    14821 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga cgtggacgct
    14881 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    14941 gattattgcc ctgatgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    15001 acagcaaaaa caacagcgcg aggaagccat tgatccgctc agtgtggaac tgaacgccca
    15061 gcagcgttac ctcgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    15121 cctgtggcaa ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    15181 gctgcgcgag gggtttccgt cagacattat ttatgccccg gagggctcgc gcggcgcaga
    15241 acaacgcctg taccttacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    15301 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacataccct
    15361 cggctggctg aaggaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    15421 tttacccgat ctgctgaccg cagacaagcg ccgccgtgag tatctgctac agacgctgcg
    15481 cagccgcttg caggatatac gccagcatct tcactgccag ctaccggttt acgtggtact
    15541 gacccggctt gatttattgc agggttttgc cgccctgttc cagtccctga acagacagga
    15601 tcgtgatgcg attctgggtg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    15661 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    15721 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    15781 gggaagccgt gaacccctgg tgtcactgct tgaaggtctg cttgacggcg aaaatatgaa
    15841 cgtgatgctg cgtggtgtct atctcacctc gtcactgcaa cgcggccaga tggatgacat
    15901 ctttacccag tccgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    15961 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    16021 gcctaatctg gcaacggaga gccgtgactg gctgatgcgt tcccgtcgcc gcctgacggt
    16081 tttctctgcc acaggcggcg tggcggcact gctgctcatc accggctggc atcactatta
    16141 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    16201 gccgcctccg caggggaaag acgactacgg caacctgcaa ctgccgctgc tgaacccggt
    16261 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    16321 actgtaccag ggacgacgta tcgggcctta tgtggaacag acctatctgc aactactaga
    16381 gcagcgttac ctgccctcgc tgtttaacgg gctggtcaaa gcgatgaacg ccgcgccgcc
    16441 ggagagtgag gaaaaactcg ccgtgctgcg cgtgatgcga atgctggagg acaaaagcgg
    16501 acgcaataat gaggtggtga agcagtatat ggcaaaacgc tggagcgaaa agttccacgg
    16561 ccagcgtgat atccaggcac aactgatgtc ccatcttgac tacgcgctgg ctcatactga
    16621 ctggcatgca gagcgtcagg cgggcgacgg tgacgccatc agccgctgga tgccatatga
    16681 caagcccgtg gtatcagcac agaaagaact gagcaaactg cctgtctacc agcgggttta
    16741 ccagagcctg aaaacgcggg cgctgggcgt tctacctgct gacctcaatc tgcgtgacca
    16801 ggtagggcca acctttgacc aggtctttac atctgccgat gacaacaaac tggttgttcc
    16861 acagtttctt acccgttacg gcctgcaaag ctattttgta aaacagcgcg atgaactggt
    16921 tgaactgacg gcaatggatt cctgggtact caacctcacc cgcagtgtga aatacagtga
    16981 cgccgaccgc gcggaaatcc agcgccagtt gaccgagcag tatatcagcg actacaccgc
    17041 tacctggcga gccgggatgg acaatctgaa tatccgcaat tttgagtcca ttggacaact
    17101 gaccggggcg ctggagcagg ttatcagcgg cgaccagcct ttgcagcggg cgctgactgt
    17161 gttgcgtgac aacacacagc caggcgtctt ttctgaaaaa ctttctgcca aagaacggga
    17221 tgaggctctg gcagaaccgg actaccagtt gctcacccgc ctcgggcatg aatttgcccc
    17281 ggaaaacagt actctggcag tacagaaaga caaagaaagc acgatgcagg ccgtgtatca
    17341 gcaactcacc gagttgcacc gctatctgct ggcaattcag aacgcgcctg taccagggaa
    17401 atcggcgctg aaagccgtgc agttgcggct tgatcagaac agcagcgatc cgatattcgc
    17461 cacccgccag atggcaaaga cactgcctgc tccgctcaac cgctgggttg gcagactggc
    17521 tgaccaggcc tggcatgtgg tgatggtgga ggccgttcac tatatggaag tggactggcg
    17581 cgacagcgtg gtgaaaccgt ttaacgagca actggcaaat aactatccgt ttaatccgcg
    17641 ttctgcacag gatgcctcac tggatgcctt cgaacgcttc tttaaaccgg atggcatact
    17701 ggataccttc taccagcaga acctgaagct gtttatcgac aatgacctga gtctggagga
    17761 tggcgataac agcgtcatta ttcgcgaaga tattattgcg caactgaaaa ccgcgcagaa
    17821 aatccgtgac atcttcttca gtaaacagaa cggtctggga acatcctttg ccgtggaaac
    17881 ggtatcgctc tccggcaaca aacgccgcag tgtactgaac cttgacggcc agttagtcga
    17941 ttacagccag gggcgcaact ataccgccca tctggtctgg cctaacaata tgcgcgaagg
    18001 caacgaaagc aaactgacgc tcatcggcac cagcggcaac gcaccgcgca gtatcagctt
    18061 cagcgggccg tgggcgcagt tccgcctttt cggggccgga caactgaccg gagttcagga
    18121 tggcaacttt accgtgcgct ttagcgtgga cgctggcgcg atgacctacc gtgtgcatac
    18181 cgacactgag gataacccat tcagcggtgg gttattcagc cagtttggtc tgtcagacac
    18241 actgtactga ggaggcaggg atggatagta acggactgac acaaactatc gttaccggca
    18301 gtgacccgcg cgggctgccg gaattcagtg ccatccgcga ggaaataaac aaagccagcc
    18361 acccgtcaca gcctgagctg aactggaaac tggtggagtc gctggcactg gcgattttta
    18421 aagccaacgg tgtggattta cacaccgcca cctactatac gcttgcccgg acacggacac
    18481 agggactggc gggattctgc gaaggtgcgg aactgctggc ggcaatggta agccacgact
    18541 gggataagtt ctggccgcag ggcggcccgg cgcgtactga aatgctggac tggtttaact
    18601 cccgaaccgg caatattctg cgtcagcaaa tctcctttgc ggaatccgac ctaccactga
    18661 tctaccgcac agagcgggca ttgcagctta tctgcgataa gctccagcag gtggaactga
    18721 agcgcgttcc gcgcgtggag aatctgctct attttatgca gaacacgcgt aaacggcttg
    18781 aaccccagct gaagagtaac actgagaacg ccgcacagac cacggtcaga acgctgattt
    18841 atgccccgga aacacaggca tcttccacac cagaagcggt agtgcctccc ctgcccggcc
    18901 tgcctgagat gaaagtggaa gtgcgcagtc tgacagagaa tcccccacag gccagtgtga
    18961 taaagcaagg cagtacggta agagggttta tcgcagggat cgcctgttca gtggctgtcg
    19021 cctcagcatt gtggtggtgg caggtctatc cggtgcagca gcaactgtta caggttaacg
    19081 acaccgctca gggcgcagca acggtgtgga tggcctcacc tgaactcgaa aactatgagc
    19141 gcaggctgca acaacttctt gatacctccc cggtacagcc gctggaaacc gggatgcaga
    19201 tgatgcgtgt tgccgacagt cgctggccgg aaagcctgca acagcaacag gcctcgacac
    19261 aatggaatga ggcactcaaa acccgcgcac agagtagccc gcagttgcgt ggctggttgc
    19321 agacccgcca ggacttacat gcttttgcag atctagtgat gcagcgcgag aaagagggac
    19381 taaccctttc ctatatcaaa aatgtcatct ggcaggcgga gcggggactg gggcaggaaa
    19441 cacccgttga gtctctgttg acgcagtacc aggatgcccg tgcgcagaag cagaatacag
    19501 atgcgctgga aaaacaaatt aatgagcgac tcgaaggcgt gttaagccgc tggctgctgc
    19561 tgaagaataa cgtcatgcca gaggcggcaa ccg
//
LOCUS       DAZGCS010000091        19541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGCS010000091 DAZGCS010000000
VERSION     DAZGCS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.091"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..138
                     /locus_tag="ACHK01_003551"
     CDS             <1..138
                     /locus_tag="ACHK01_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO8098449.1"
                     /translation="YRWHGSAEAHTCREVQDIPGVLAVFAERRKDSFGLYVRLMSVTL
                     N"
     gene            301..669
                     /locus_tag="ACHK01_003552"
     CDS             301..669
                     /locus_tag="ACHK01_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8098450.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            759..1085
                     /locus_tag="ACHK01_003553"
     CDS             759..1085
                     /locus_tag="ACHK01_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8098451.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDN"
     gene            1133..1621
                     /locus_tag="ACHK01_003554"
     CDS             1133..1621
                     /locus_tag="ACHK01_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8098452.1"
                     /translation="MKLALTPEADSVNVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPSFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRQGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1633..1830
                     /locus_tag="ACHK01_003555"
     CDS             1633..1830
                     /locus_tag="ACHK01_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8098453.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            1915..2757
                     /locus_tag="ACHK01_003556"
     CDS             1915..2757
                     /locus_tag="ACHK01_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8098454.1"
                     /translation="MSDITISRPELVNRHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTAPAWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYQSLEYDNFPEISEANILSTFEQLHQNKDEVFERGMINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQCKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAM"
     gene            complement(3100..3636)
                     /gene="yghD"
                     /locus_tag="ACHK01_003557"
     CDS             complement(3100..3636)
                     /gene="yghD"
                     /locus_tag="ACHK01_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO8098455.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIADRGENIQVWIDPVVFNDLLKWLNALDEKYALRVTQIDAS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(3638..4816)
                     /gene="gspL"
                     /locus_tag="ACHK01_003558"
     CDS             complement(3638..4816)
                     /gene="gspL"
                     /locus_tag="ACHK01_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8098456.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILQILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(4813..5790)
                     /gene="gspK"
                     /locus_tag="ACHK01_003559"
     CDS             complement(4813..5790)
                     /gene="gspK"
                     /locus_tag="ACHK01_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8098457.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5793..6392)
                     /gene="gspJ"
                     /locus_tag="ACHK01_003560"
     CDS             complement(5793..6392)
                     /gene="gspJ"
                     /locus_tag="ACHK01_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8098458.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6389..6760)
                     /gene="gspI"
                     /locus_tag="ACHK01_003561"
     CDS             complement(6389..6760)
                     /gene="gspI"
                     /locus_tag="ACHK01_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8098459.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6757..7320)
                     /gene="gspH"
                     /locus_tag="ACHK01_003562"
     CDS             complement(6757..7320)
                     /gene="gspH"
                     /locus_tag="ACHK01_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8098460.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7324..7779)
                     /gene="gspG"
                     /locus_tag="ACHK01_003563"
     CDS             complement(7324..7779)
                     /gene="gspG"
                     /locus_tag="ACHK01_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8098461.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7796..9019)
                     /gene="gspF"
                     /locus_tag="ACHK01_003564"
     CDS             complement(7796..9019)
                     /gene="gspF"
                     /locus_tag="ACHK01_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8098462.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPAMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFSP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9019..10512)
                     /gene="gspE"
                     /locus_tag="ACHK01_003565"
     CDS             complement(9019..10512)
                     /gene="gspE"
                     /locus_tag="ACHK01_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8098463.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(10512..12572)
                     /gene="gspD"
                     /locus_tag="ACHK01_003566"
     CDS             complement(10512..12572)
                     /gene="gspD"
                     /locus_tag="ACHK01_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO8098464.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(12602..13561)
                     /gene="gspC"
                     /locus_tag="ACHK01_003567"
     CDS             complement(12602..13561)
                     /gene="gspC"
                     /locus_tag="ACHK01_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8098465.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(13579..13989)
                     /gene="gspS2"
                     /locus_tag="ACHK01_003568"
     CDS             complement(13579..13989)
                     /gene="gspS2"
                     /locus_tag="ACHK01_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO8098466.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(14055..14864)
                     /gene="pppA"
                     /locus_tag="ACHK01_003569"
     CDS             complement(14055..14864)
                     /gene="pppA"
                     /locus_tag="ACHK01_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO8098467.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(15012..>19541)
                     /gene="sslE"
                     /locus_tag="ACHK01_003570"
     CDS             complement(15012..>19541)
                     /gene="sslE"
                     /locus_tag="ACHK01_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO8098468.1"
                     /translation="LAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPN
                     PEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESS
                     DGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKS
                     NALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNA
                     ATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGY
                     GVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEV
                     RGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQV
                     VNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGV
                     DTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKR
                     AWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPHYNSILRC
                     PNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTVGTNLDTV
                     YFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYVTQWGSDP
                     YSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAG
                     LSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQ
                     QENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQRILDAFPGLEV
                     CKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQAADLGTNIQRL
                     YQHELYFRTNGLQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKEDELGFKTFTEFL
                     NCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYMEKPLTRLMLGR
                     SWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTI
                     KSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYI
                     KGDSKDNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTAPKKNLNAS
                     NYTGGLKQFANDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFANDVQISIGD
                     AHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALY
                     MQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKK
                     WYPEGSLPAFYSEREGMKGWNLFQLMHRKARGDEVGKTKFGERNYCAESNGNAADTLM
                     LCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVGQSAYNTLASLKLPKPEQG
                     PETINQVTEHKMSVE"
BASE COUNT         4412 a         5521 c         4929 g         4679 t
ORIGIN      
        1 taccgctggc acggcagcgc tgaagcacat acctgtcgtg aagtgcagga tattcccggc
       61 gtgctggctg tgtttgccga acgccgtaag gacagttttg gcctgtatgt ccggctgatg
      121 agcgtcaccc tgaactgatg ttccgtcacc cggaaaacga gtacgactgg ctgagctcag
      181 acgccgctgt ggatgaggtc ctgctcatca acgaatacac ctttaccgaa gccggtctgc
      241 gcgccggctg acccgtcatc gttaaactat cattcaccga ttttctgtga ggattttatc
      301 gtgtcagaca cactctccgg gacaacgcat cccgatgaca atcacgacca tccctggtgg
      361 ggactaccct gcacagtgac gccctgtttc ggcgtctgtc tggtgcagga gggtaaccgg
      421 ttgcattacc ttgccgaccg cgccggtatc agaggcctgt tcagcgatgc agatgcgtac
      481 cacctggacc aggcctttcc gctgctgatg aaacaactgg agctcatgct caccagtggt
      541 gaactcagcc cccgctatca gcataccgtc acgctgtatg cgaaaggact gacctgcgaa
      601 gccgataccc tgggctcctg tggttacgtt tatctggctg tttatccgac gcccgaaatg
      661 aaaaattaac tctccagaat agccttctgc tacggcctgg tgttttcacc acgccacttt
      721 cccatttctc atctctgcat atcaggaaaa ccttcagtat gaaaacatta cccgatacac
      781 acgtacggga ggcatcgcgc tgcccgtctc ccgtcatcat ctggcagaca ctgctcaccc
      841 gactgctgga ccagcactac ggcctcacgc tgaatgacac accgttcgct gatgaacgtg
      901 tgattgagca gcatattgag gcaggcattt cactgtgtga cgcggtgaac tttctcgttg
      961 aaaaatacgc gctggtacgt accgaccagt cgggattcag cgcctgtacc cactctcagt
     1021 taataaacag catcgatatc ctccgggctc gccgggcaac aggcctgatg actcgcgaca
     1081 attaaagaac ggtaaataac attaccctgg gtaaacatcc aggggcgaaa caatgaaact
     1141 ggccctgaca ccggaagccg acagcgttaa cgtacaggca ctgaacatgg ggcgcattgt
     1201 cgttgacgtc gatggtattg agcttgctga actgattaac gtggtctgcg ataacggtca
     1261 ctcccttcgt gttgttgatg aatctgaccg agcctcaaca gactgcacgc catcatttgc
     1321 tgccctgact ggcatacgct gcagtaccgc gcatatcacg gcaaaagaca acgcctggct
     1381 gtactcgctg tcgcatcaga cgaatgacac cggcgaatca gaatggattc attttacagg
     1441 cagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaagcgcca
     1501 gggcctgtca aaaacgttcc gccgtctggt tgtcacactc atccgacgtt atggcgtcag
     1561 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     1621 accaggaaca acatgaaatc attaaccacg gaaaccgcac tggatattct gattgcgtgg
     1681 ctgcaggaca atatcgactg cgaatcggga attatctttg acaacgatga ggataaaaca
     1741 gattcggcag cactgctgcc ctgcattgaa caggcgaggg aggatatcca taccctgcgc
     1801 caactgcagc ttctgcaaca gaaccggtga gtcttactca ccagacttca ttccactgac
     1861 gccagcctga atacggctgg cgttttcgtt tataaaccaa aaggaaaaac gatgatgtca
     1921 gatatcacaa tctcccgccc ggaactggtc aacagacata cggacgttat ctgctcaacc
     1981 agtatccgcc acattttggc tgtacgaaag agtgcgctgc tgcaaatcga cacacttatc
     2041 cggcaactgg ctgaaatctc agcaatgaca gaaagtattg gcggtaaaac cgccccggcc
     2101 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc ggaaaccgca
     2161 atgaaagcca tcacccggaa tcttgaccgc gaaatctggc gtgacctgat gcaacgttca
     2221 gggatgcttt ccttaatgga tgcacaggcc cgtgatacat ggtaccagtc actggagtac
     2281 gataattttc cggaaatcag tgaagcgaac attctgagca catttgaaca actgcaccag
     2341 aacaaagatg aagtgtttga gcgggggatg atcaacgtct tcaggggact gagctggaat
     2401 tacaaaacca attgcccctg caagtttggc agtaaaatta tcgtcaacaa cctggtaagg
     2461 tgggacagat gggggtttca tcttatcacc gggcaacagg ccgatcgact tgccgatctg
     2521 gaaagaatgc tgcacctgtt cagcggcaaa ccgatccccg acaaccggga aaacatcacc
     2581 attcatctgg atgaccacat ccagtctgtt cagtgtaaag aggactatga agatgagatg
     2641 ttcagcatca gatactttaa gaagggctcc gcacacatca cgttcaggaa accagaactg
     2701 gttgacaggc ttaatgatat tattgccaag cactatcccg gagcgttagc gatgtaaaaa
     2761 ggaataaaac tcaggaataa aacccacaaa aatacacgtg ttctgatgac aaaaatcttg
     2821 atctacttgt tctcataaaa accaccagaa gggaccgagg gtacaagaga gggtacattt
     2881 ttcatcaaac caaataaacg agattaatat caacatgata acaaccatat tcgattccga
     2941 gtccggcacc actaattctt aagaacccgc ccacaaggcg ggtttttgct tttggatctg
     3001 acaataacct tcacgaaaaa aattagctta taaagtctgg gggaattact ctcgccacgt
     3061 taacgagagt aattttattg atattaatct cctgatactt tacccccgtc caaactccag
     3121 ccgctgcaca ttcaccatcc caggcttctc agcagcactg gcatcaattt gtgtcacccg
     3181 cagcgcatac ttttcatcca gcgcgtttaa ccatttcagc aggtcattaa acaccaccgg
     3241 atctatccag acctgaatat tctccccacg atcggctatc cgcttgatga ctaccgagtg
     3301 cgcggaagca ctgtcactga tgacccgcga tacctgcgca ggcgttgttg tggcagattt
     3361 tcgcgctgca ataatatccg gcgcggcgtt cttcagtcgg gtgttcatcg ccaccagttg
     3421 ctgcaacatt gtctcctgtt gctcaatccg ttcgctcaaa ggctgccaga tgaggacgta
     3481 atatccgacg ctaaacagga acaccaccgc tgccagtaac atgccctttt cacgcggaga
     3541 acgccccgcc aggtgttgtg ccagccagtg ttcgccacgg cttaactggc gttcacgcca
     3601 ttgctgaaaa tagtgaataa atttatcgcg taacatgtta tttcctccgc aacgttacgc
     3661 cgccggaaac cgcatcaccc tctttctgta acgcgtcctg ttgcacaaca taatcggttg
     3721 ccagtgcact acgcagtttg tcgaaactgg caaagttcgc ggcccgtagc tggaggtgaa
     3781 gtatctggcg tttttgatca aaggtaaaac cacgcatttc gatgtcggaa agtgacgctg
     3841 atttcagggt gctggctatc gctgacaact cggcgagcag ccgggtatcg tcggcctgtg
     3901 ggcgatattt tttcagcgcc atcgtcacct gagagcgtaa attcacaatc cgcttctgct
     3961 ccgggaacaa cgttaagaac tgttcctccg cctgggtgcg gctttgcgcc acctgttcgc
     4021 tgacgctcca taacgtcacg ccccgctcca ctgccagcgc aaccagaatc agcaatatct
     4081 gcagaatcat cacccgccag cgcgcccact gttttcggta gctgacgcga ggctgccacg
     4141 gccctgtcag caggttccct tccggtttgc cataagtggt aacggcgggc agagctgtaa
     4201 cggtcaggcg ttcagcgtct gcgccagccc atgctgatag cttttccggt gcaatgccga
     4261 ctacggttag cgaaagcggt aaatcctgct cattgagctg ggcgcggaac atgaccggag
     4321 ccagcgccag cccggcgctc catccccggc attcatcgat gcggcagata acccgttgcg
     4381 catcgcaagc cataaaccca caaggaatgg acatccagtc cggcgcgacg gtagcgcggg
     4441 tgatgccgtt ttcctgcaac cactgcgcaa tgttgcgcat atgctgttgg tgaatcactg
     4501 ctacggttgc cagttgctgg tcgatattca acggggcgaa atgcagttca tcgatatcct
     4561 ggttcagctc ttcttccagt aaggcgggca gaatggtcgg tatctgctta cgggcgacat
     4621 caggcagttc aacctgccag acgctgatcc attcgccggg aatgtagagt cgaatcgcat
     4681 cggtttgcag ccattgctgg agctgttcgt tagcaacgtc agaccagata ccgtgctcca
     4741 catcgggtgt acggcgctgc caatggatgg gatcagcggc gcaaagcggg aaaaaaatct
     4801 caaggatgga actcactcac tttctcctgt ctgatgccag agaaccgaaa agtgttgtgg
     4861 gcccatgcgg acaattaacg agttcatcgt cagttcagtc tcattcacgg tgatatctga
     4921 acgcagccag aagtaattgc tgtccacgct caggacggtt tttagctgtt ttttagtacg
     4981 ctcatcgacg tcagcaagta gcggctgtgc aagaaactga tcgacatctt cccagccctt
     5041 cgccggacgt tgttgtaata acgcccgcgc ctgaacgggg cttaaccacg ggtcaaacag
     5101 cgcctcaaga atcacacttt gcgtgacgtc taaagtattg atgttgattt gctggcggat
     5161 catcggcagc gcacagacca gcggtttcag tttttgataa agcccggcgt ccattccctg
     5221 taccacgcgc atctcactga tatcagccag cggttgattc gcggcgtaga aaggcaccga
     5281 acgggcgaga tactcgctgt cttcacggcc cagacgtgtc tgcacgctgc ggtcctcgtc
     5341 aataaactcc cacaggcttt cggctatcag ttcggcccga taagcaggca catccaggcg
     5401 cgagatcagg gcaatcaact gttgtaccgc aagcggacgc gacgccgttg tcggctgagc
     5461 aagggcattc aggttaaagc aagcctgtgc gtcacgcagg gtgaccgcaa tttgcccctg
     5521 cggcagaggg aagaaacgag ggccggatgc ccacggctgt gccagatgca cgcgcttttc
     5581 atttttcagg ctcagactga gcgcgctcag cgccaggctt tccgcactgg cgctgtacca
     5641 cagcgcctgc tggtactcct gctggctgcg cgttcgccca agttgttgct gcatccgccc
     5701 ggaaagcgtg atggttacca gcatcataac cgccagcaat accagcacca cgaccagtgc
     5761 cattccgcgt tttggtggtg aggtgatcat gattattgcg gcccgcgtaa caaccagatg
     5821 cgttcaatct cgccccattg cggcgaatgc agggtcatgc gtaccgccac agggatcgcc
     5881 tgtactgacg accagctctc ctgccagcgc gtgccgtcgt agaactgcaa acggagcgaa
     5941 tcggccggaa tcaacttttg cattgtcggc ttcacgctgc ctgcggcatc ggtcagcggc
     6001 caggctaacc gttcgagata accgtcatga atgcggtaac cgacggtgag cagattactg
     6061 cgcggcagac gcatcaacgg gttaaccaca ccgccgcgca caaaacgcat tccttcactc
     6121 tcagacgcca gcacgccagc gccagccagt aacgctggtt cacgctggcc ctgatcgcct
     6181 cttaccggac gcggcatcat ttgggtcaga tcgtgggtca gaaaactcat cgtttgctgc
     6241 atgaggttta gtttttgatc gtgtccggcg acggcgctat tcacgcgcgt gacgccgtta
     6301 gtcacctgct gcgccatcag tgccagcgag gcaaaaatgg caattgccac cagcatttcc
     6361 agtaacgtga aaccagcgcg agtccttctc actgttggcc tcccacggcg ctaaaccagg
     6421 cgcgtcgtga ctgaatcacc gacgaaaagt cttcgtgacg gctgacttca atatccaccg
     6481 cctggagcag cgcgttgcct gtattcaatg ggattgttcg ccagtaccag cggcttcctg
     6541 ccatatcgct ctcaccctga tgcctgaccg cccgctgctc acgggtcatc aggtagagca
     6601 gcgcagtctg gttttcagct acccagcccg ctaccgtttt ttcctcaaga acgtgctgat
     6661 tactcagcgc accgctggca atctgtaaca ccgccgtggc agccagcgca aaaatcgcca
     6721 gcgcgagcat cacttccagc aaggtaaatc cgcgcttcat ggcatcctct catcacattg
     6781 attgagggat aacgcgccat cgtgtgccag ctttaccgcc caacatgcgt tttgcgccgc
     6841 cgagtagaag cgcagcgtaa acggcgtggc gggttcaaaa ggcgaaaaac ggatctgcgg
     6901 cgtcttcttt ttcgcctctt tttgcagctc aagttcaata tcgtgcagcg tcagtcgacg
     6961 acgttgcagc tccagcgcat attccttctg ccagatatca cttccgggtt gtaacagcat
     7021 ctgcacctgt ttcggcaccg taacctgtgc cgataaccgg gtcgaggaaa cgggcgacca
     7081 ctgcccctga cgacgctgca taaactggta gccaggcggg tcgattagca caccgagtgt
     7141 tttcccttcg atcactgccc tgtccttaaa ctgcgcaaag cgagtcagaa aatcctgcgc
     7201 cgcttttttc gcgggcgatt cagaatcggt cgcaaacgtc tgtactacgc ccgcactggc
     7261 aaggccgata aggaaaatca ccagcatgat ttccagaagt gtgaatccgc gttcaggcac
     7321 tgcttactga aactcctgca aattccagtt accgatatct gcgccagcgc cttccccgtt
     7381 ttcctgacca tccgccccca gggtataaac atcaaacagc cctttttcac ccggactgag
     7441 atactgataa tcattgcccc acggatcctt tggcagtcgt ttaatgtatc caccagtacg
     7501 gtagttgcgg gagtccgcca tattggctgg ctgctggatc agcgcctcaa gcccctgttc
     7561 tgtggtcgga taacgcccgt tatccagtcg gtacatatcc agtgcgtttt ccagcgccac
     7621 gatatcgctg atggcttttt gccgatcggc tttctctttg ttgcccaaca ggttaggcac
     7681 caccagactt gccaggacgc caagaataac aatcaccacc atcacttcca gcagggtaaa
     7741 acctgcccgt ggtttttgtg tgcgggataa cgaattcatg tgataactcc gtaaattaca
     7801 ttccaaccat attgttcagt tgcagcatcg gctcgaggat ggcgatgaca ataaacagca
     7861 ccacgcccgc catcatcacc accagcgcag gctcaaacag ccccaacgcc agccccacct
     7921 gggtatcaaa ttcccgttcc tggttgactg cggcctgctc aagcatggtt tccagttcgc
     7981 cgctctgttc gccggaggcg atcatgtaca gcatcattgg cgagaacagc cgcaactccg
     8041 ccagcgcggc gcgcagactg cttccttcgc ggacgcgatc tgccgccagc agtagttgtt
     8101 gttcgacata gcgatttgcc gacacggcag cagcggtctg aatgccttcc agtagcggaa
     8161 cactgctggc ggtgaggatg cttaaggtgc gggaaaaacg cgccgtattc agcccgcgcg
     8221 caacacgccc cgtcacaggc aggcgcagca cggttttgtc ccagcgcagg cgcatagcag
     8281 gatttttaag tagccgttgc cccagcacca gaagcgccag caatcccgcc agccagtaaa
     8341 cgccgctggc ctgtaacgca tcactcatgg caataagcgt gcgggtggag gagggtagcg
     8401 cgtgtccgag atgatcaaac tgttcgatga ttttcggcac cactgccgtc agcaaaatag
     8461 tgactacgcc cgttgccacc accagcagaa ccagcggata gagcatggcc tgcaatagac
     8521 gtgatttcag gcgctgtcgc tgttcggtgt aatctgccag gcgattgagc accacgtcga
     8581 gatgcccgga tttttctccg gcagcaacca tcgaacaaaa cagagaatca aagacgcggg
     8641 gatgttcgcg caggctgtcc gacagggtgt aaccttcctg aatccggctg cgcagcgcca
     8701 ttccgaggct ttttacatgc agtttttcac tttgctcact gaccgcctgt aagcaggttt
     8761 ccagcggcat tgctgcctgt accagcgttg ccagttggcg cgtgaacagc gcaagatctg
     8821 ccgccgccac gcgacgatgt gcgtgccgcc gacgctgcaa catcccccct gacgaagtat
     8881 tcatccgggc ttcaatatgc acggggataa gctctttacc gcgcaacaac tggcgggcat
     8941 gacgcgcgga atccgcctca atcatacctt tggttttgcg accattacgc tccagcgcct
     9001 gatagtaaaa cagtgccatt acgcctccat ggttacccgc agaacttcat cgagagaggt
     9061 ttctccggcg agcactttct caatgccgtt gctgcggata cccgcagagt gttgtcggac
     9121 ataacgttcc agctccagct ccccggcctg acggtggatc aaatcacgca atgtggcatc
     9181 caccacgatc agctcatgga tggcagtccg tccgcgaaaa cctttgtgat tacaggcggg
     9241 acagccctgt ggatggtaca gagtgacggt acgggcgtcg gtaattccca gcaggcgttt
     9301 ttcttcgtca gtggctggtg cgggctggcg gcagtcggga cacagcgtgc gaaccagtcg
     9361 ctgcgccatc acgcccgtca gactggaaga gagcaggaaa ggctccacgc ccatatcctg
     9421 caaacgtgtg atcgccccca ccgctgtgtt ggtatgcagc gtggaaagga ccaggtgtcc
     9481 ggtaagtgaa gcctggacgg cgatttctgc ggtttcggta tcgcggattt caccgaccat
     9541 caccacatcc gggtcctgac gcaaaatcgc gcgcagccca cgggcaaagg tcatgccgac
     9601 gcgggtgtta acctgcgtct gaccgatccc ttcaatcatg tattcgatgg gatcttcaac
     9661 cgtgagaatg ttgcgcgaat ggttgttcag ctcctgcaat ccggcgtaca gcgtggtgct
     9721 tttgccggaa cccgtcggcc ccgtcaccag aaagatgccg tgcggtttgt gtaacagctg
     9781 gcgcaactgc gcggtcagtt gctggctaag ccccagacgc tccagcgtca ggcgggcctg
     9841 gtttttgtcc agcagtcgca gcaccacgcg ctcgccccag gcagaaggca tggtggagac
     9901 acgcacgtca atcgcccggc cgcccagcaa cagcgcaata cgtccatcct gcggcacgcg
     9961 cttttcggca atgtccagcc gcgccatcac cttgatacgc gacaccagca gcgaggccag
    10021 tttgcgcccc ggacgcagca tttcatgtaa tgtgccgtca acacgaaaac ggatcaccag
    10081 actcttttca aacgtctcaa tgtggatatc cgaagcgcct tctttgattg cctctgccag
    10141 catggcgttg atcagtttga tgatcggcgc atcgtcgtcg ctttccagca gatcttccgt
    10201 ttccggcagt tcttccgcga gggtaaaaaa gtcctcggca gaaccgagat cttccatcag
    10261 ctggcgagcc tcggaagagt cccgctgcca gaccgcattc agccgctgtt caaattcggc
    10321 ctcgtcgatt tgccgcagcg taaagggcgc gttcagcccc cgctgcagct cctgcaaaac
    10381 agagagcgat agcggatgaa cgtggaggat ctccagcgac gcttcgcacc atgccaccag
    10441 gctaaaccga cggctgaaac tgtagggcag acgcacggtg ttggcggtgg tttcctgtgc
    10501 tacaggcacc attaacgcgt tctcccggca ttgaggaacg cgcgaacttc cggcggtaag
    10561 gcctggtttt gcgctggcag taccggctgt gcggtgtgcg gcatcaggct taaaccttgc
    10621 tcatcacgat agatctgttc ggcgcgcata tagttatatt tgcgctgcga cacgccgtct
    10681 gccgccatac cgtcacgcag aatggtcgga cggataaaca ccatcaggtt acgtttttct
    10741 tttttatccg ccgtcgattt aaacaggtta ccaatcaatg ggatatcgcc cagcagcggc
    10801 actttcgcca cgctttctcc cgcctgatcg tccatcagac caccaagcac aatcagctca
    10861 ccatcgttag ccagcacggt ggttttcagt ttgcgctcac caaacaccac gtcgaggctg
    10921 gtctgtcctt ccaccttcga gacttcctgc tcaatcacca tctgtaccgc gttaccttcg
    10981 ttaatctgcg gcgtgacttt cagcatgatg ccgacttttt tcctttctac tgtattgaaa
    11041 ggattgctgt tattggagcc aacggtagag ccggttaata ccggaacgtc ctggcccacc
    11101 atgaagaagg cttcctggtt gtccagcgtg gtgatgctcg gcgtggagag cacgttcgag
    11161 ctggagtcgt ttttaaccgc ctgcaccagc gccatccagt cgcctttcac cacaccaacc
    11221 gccgtaccgc taaagccgga aagaagctgg gcgagtgtgg agagatcgcc gttggtatcc
    11281 ggattaatgg tggtagcgcc gttttcactg atcaccgtcg agcctttctg cggtttcgcc
    11341 tgagaaatgg ctgcccccag cgtaccgata gggatctgcg taccattagc aaactgcatt
    11401 aatccggcat ctttcgacgc ccactgcacg ccgaagttga tattgctacc ttcggcaact
    11461 tccacgatca acgcctcgac gtgcacctga gcacggcgaa tatccagttg ttcaattacg
    11521 ctttgcagcg actgcatgat gtcctgcggc gcggttacaa tcagggcatt actgtgtttg
    11581 ctggcggcga tggatacaac ctcacgcccg ctaccgaccg tgccttccgc ctcttcttta
    11641 gccgccgtga gcgtgccgct gacctgtttc agcacatcga ccagatcttc ggctttgctg
    11701 tatttgagat agaaaacctg gctgttgccg ctgcgttcca tttctgagtc cagccgacgg
    11761 atcaggcggc gcattttgtc ccgcgtggcc gggtcaccac tgacaatcac actgttggtg
    11821 cgttcgtcgg cgacaatttg agatttcagc gtcgcaggct ggttctcgcc gctgtttttc
    11881 gtcaggcttt ccagcacgcg ggcgatttcc gaagccgagg cgttatccag cgggatcacc
    11941 tcttcggtgc gattacccgc gtgatccacg cgctggatca cttccgtcag gcgctccaca
    12001 acggaggcgc gtccggtgag cataatcacg ttggagggat cgtaattaac aacgttgcct
    12061 gagcctgcgc tgtcgatcat ctggcgcaga atcggtgcca gctcgcgtac cgacacatta
    12121 cgtaccggca ctactttggt gaccatttca tcgcccgcgt agttgtcgct gccttcaccg
    12181 accagcggca gcggctcgac tttcgcagcg ctggatttca ccaccttcag cacgtcgttt
    12241 tccatcggta cgacggcata cccctgtgct tccagcaggt taaggaatag ctggtaatac
    12301 tggcgttcat tgagcggggt catggtgcga atactcactt tcccctgtac gcccggcccc
    12361 ataatgatgg ttttattaag gttagcgccg acggtttcga tgaacgattt caggtcggta
    12421 tctttaaaat tagcggtgaa agtggcttct tccgcccaga ccggtgaact gcataatgcc
    12481 gctgccagca ccagcggcag taaacgcttt tttgttttga ggccagttgt cttcttacgc
    12541 cagaccgaca acgtaatatc acgccaaaac acgatgaatg attctcctgg attaaatgcg
    12601 gttagcgcag cgcgatggaa atgtcgtggc gcgcaccctt gcgtaaaacc gtaagttgaa
    12661 tggaatccat tgaaggtaac tgccgcatca gagcaatcat tgctcgtgga tcagtgaaat
    12721 cctgctgatt tagcgcaatg gcgatatcgc cttccttgaa accgctggca tcgaacagag
    12781 aacgatctgc ccccggcttc actgcataac cgacaattcc ctccttacgc acaggcgtaa
    12841 gctggatata gttaaaaatt ttctgcggat ctttcgccag tgcctgacgc acggcagtcg
    12901 ggatctccac tggcgcactg acagcaggtt cagcaacagc ttgctttgct tcgtcactga
    12961 cggctttttt gttggtcacg gctacggtgg aatgcccctc ttctgccagg ctcaggcgct
    13021 ctatttttcc ctgatagcgc agcatcacat ggtcgcgatt gatttcctca atcaccgcat
    13081 tgtgcgagcc aagcgtttca ccctgcaaat agacctgctg tttaccgcct tcttcaataa
    13141 ccgcgccggg tctggcacca aaggcaatcc cgcgcagcac cacattaaga cgcgtttctg
    13201 ccacaggcac aggttcgggt tgttttaccg gcgtggcgac gggctgatat ttgccaaacc
    13261 agttttgctg gctgattaat tgcacgtcat ttttatcgaa cgtttttgca tccgcacggg
    13321 gcggtttatt cgccgatggg gaaacagccg tatattccgc agaaaaggag aaatagcgcc
    13381 agagtgaatg cgccactttt gcagaaataa taagcagcat cagccagaac atcccgcgta
    13441 caatttttcg cagatgctct ttgtctgtat taaaagattg tctctggtta agagtgtgac
    13501 gaatttttgt cagccattga atgagataaa ttcgtgcgtc acgaaaaaca acccgcgcca
    13561 atttatatct acccgacgtt atgctttgac tattccacag gtggtacgat ccagtttccg
    13621 ctgatactgg ttacctgtac gtgtatcatt aatcgttatg ctgtaattaa ttccctcggt
    13681 aatcattaat ttcaccgttg ggtcagcgca catttgtcgt tgatagctgg ttaaaaaggc
    13741 gtcaggcgtc tgcgtggtct gagtccccgc ttcgctgata atggtcattt ttaccgtcgt
    13801 gccgctactt tgtgccagca ctaaggtata tcccgcagat ttaatcggca ggttttggct
    13861 gatattttgc gcctgttttt tcgccagtaa actggcattt tcattatggc tggcacagcc
    13921 acttaataat cctgtaacgc tcaacaatag cgatattaat accctccctg gcatttgttt
    13981 tatcgacata gtaaataatc tctaaggtta ttaataagag ttaaaatgtc actttgataa
    14041 tgacgtggtt atcattaaaa caatgcctgt agataaagtg ttgctatacc gcccagactt
    14101 aaacacggtc caaaaggcag tgtggttgat cctctttttg taataatggc atatatcagg
    14161 ccgcagcatg atgcgattaa agcaacattg ggcagtgata acggccccac ccagccacct
    14221 aacgcagcga acaataatac atcgcccatg cctaatgctt ctttacgcag aactattccg
    14281 gctatccagc gcagggagta aaaagtgata aaccccacca ggacgccggt aactgcgtct
    14341 tgtagcgtta acggactctg ttgcgcccat gccgcaatca gtcctgtcca caatacgccc
    14401 tgagtaaaaa catcgggcag ccattggtga tcgaggtcga tgatgctcgc ggcaatcagc
    14461 caggcggata atatcatcac cgccagcgcc catccacttt ccggccagac cagactcgcc
    14521 agcaaaaaag cgagtgccgt caataactcc accagcggat aacgcttgct gattttcgcc
    14581 tgacagtcgc ggcagcgccc tttgagcatc aaccaggaga gcagcggaat attgtcacga
    14641 acgcggatgg tctgctgaca atgtgggcag tgcgaacgcg gtagcgcaag gcttattttt
    14701 gactgcgcac tcgacatttc accgtgaaac tccgccattt gttggcgcag catgatgggg
    14761 taacgccaaa tcaccacatt caaaaaactg ccgatgatca atcctccgac ggttgccagt
    14821 atgggcatcg ccgcggggta ttgctgaaaa acatcaaaaa gcatggttaa aggttgtttg
    14881 ttgtaacttg ctggatgcgg gcctgcaagt gtatgccata cggctttcct ccaggcccga
    14941 taagctggcg caacccggta cgccttattt catgccggat gcggcgtgaa cgccttatcc
    15001 ggcatacagg attactcgac agacatctta tgctcggtaa cctgattaat ggtttccggt
    15061 ccctgttccg gtttcggcag cttgagcgac gccagcgtgt tgtatgccga ctggcccaca
    15121 ccgccctcga agctcatctc gctcgccccc ggcagctggt aagcattcgc acccggattc
    15181 catttcttaa agaactccga aagatccgtc tgggcgaccc aggaggcaca cagcatcagc
    15241 gtgtccgcag cgttaccgtt tgactcagca cagtaatttc tttcgccaaa cttggtttta
    15301 ccaacctcat cgccgcgtgc tttacgatgc atcaactgga acagattcca gcctttcatc
    15361 ccttcacgct cgctgtagaa cgctggcaga gagccttctg gataccattt cttgatatca
    15421 aagtttttct ctgcccattc cttcagttgt gcgtacatca gcagacggtc acccgcaccg
    15481 ccgcgcgccc atgcctgacc gttgctctcc tccagatatt ccggtgcgac ggtaatatcg
    15541 tcagcgacac ggttcatctt gccgagataa cgatcctgca tgtacagcgc cagcacgttg
    15601 ttcgccactt cggtcgcgcc tggaacagtc agcggcgttt ctgcggcgtt gtgccctact
    15661 tcgtgccaga tcagccagtc gttcagcggc gtcgtcggca gcgtggtgct gttcgtcgag
    15721 aagctgctgt tcattaccgg ataacccgaa tgcgcatcac cgatggagat ctgcacatcg
    15781 ttggcgaaac gatgcttgtg gcccgtcaag tttttatagg taaacatccg gtgcttaccg
    15841 tcttcatcat tacgaccgta gaaatcattc atcgagctgg caaaggtatc cagatcgtta
    15901 gcgaattgct tcagtccgcc agtgtaattg ctggcattca ggttcttctt cggtgcggta
    15961 taaacgaaag cgtctgattc cagctcaccc agcggagccg gtgagttcag atcgtttttc
    16021 catgcgccgt ctttatagaa cggtgctttt accacaccag taaaggtgaa gctggcagat
    16081 tcattgtctt tgctgtcgcc tttgatataa atcagaccac cgtaaggcac cttgaatttc
    16141 accgtaccac tagcgtccag agagtacgtt ttggttactc ttggcggacg gttcagcgcg
    16201 acttcatgct tctctcttcc ggtcaggtcg tcagccagcg ccacggtgac ggtcacagga
    16261 acgttcgcat tggacttaat ggtgacctct ttctgagccg gtgcccacag gccagttgac
    16321 tgcatgttac ctgcaaacca tttggtcgga ttcgagtaca ggctgatggt ttcagtaacg
    16381 ttctgtccct cttccgatac tgctcccgga tacttctcga catcaacttt gatgttcaga
    16441 tcccaccagg aacggcccag catcaggcgt gtcagcggtt tttccatata gttgagcggg
    16501 tagctcgggt tcatcatgcc cgctttgctg ctttcttcac cgtaaatcat cttgttatcg
    16561 atcagcgatt ttttcagctc agcggaacac tgcgtgccaa cgtatgcatt gttggtgtag
    16621 cagttcagaa actcagtgaa cgttttaaag cccagctcgt cttctttacc ctcttcataa
    16681 cgatatttcg tctcgttcca cagccagacg gacatgtttt ggtataaacg ttccagatcg
    16741 acgctgttga gacgctcacc ctggaggcca ttggtacgga aataaagctc atgctgatac
    16801 aggcgctgaa tattggtgcc taaatccgca gcctgcagca tcgctttcgc agtgtcagct
    16861 ccaagatcca gctgcgtata ctgcggaaca tacatgccac cggttaccgg cacacccgtg
    16921 cctgggcggt attccagaca gttgacctca tagtgatagt cagaatcctt acacacttcc
    16981 agccctggga acgcgtccag aattctctgt tttgccgcag ccagtgactc aggcgttttg
    17041 tggtcggctt catcaataaa ggcgaattgg gttacctgtt taccctcaac ttcttcctgc
    17101 cagctggcaa cttccagctt cggtttgtca tcaggtttgt tttcttgctg atatttccag
    17161 ataacttctt tcgtggtgtc atcaatggta tacggtggtt taccatcgac agccggataa
    17221 cgctcataga cccaaattgg cgttgaacgt cgttgacgaa cacggtcagg gtagccttgc
    17281 ggatcgttat ttactaccga cttgttaagc gccatcgaca aacctgcggc atcaagcaga
    17341 cgtacaaagc cagatgcgct ctcttcctta agattgctca tcacgttttc catgatcagc
    17401 accgatccac ctttgttcat ataggcgatc aaatcggtca catcctgctg ggtcagcttc
    17461 ggtttgctgg tatctgcgcg cagaggaatg gagtaaggat cgctacccca ttgtgtgacg
    17521 tattcaaagc cattgaggat caacagagga acttcatctg gattcagatt gccatagctg
    17581 gtcattggtt tgaccgtgat gccagtgaaa tccttgtgga acgcaaacgg tgcgctattt
    17641 cccagcacct gaccatgttt tttgaaatat accgtgtcca ggttagtacc cacggtcata
    17701 ctggattttg catccggcaa ccagcgatca tttgacagat agcgcagcac gttctccatg
    17761 aagttcttca tgtcatccgg gtcgctgttg agcgtacact gtccgtcttt attaacgccc
    17821 ccgttccagc tgtaaccgtt cgggcaacgc aggatgctgt tgtagtgtgg gttaccgata
    17881 accatcagct tgccatcgcc cacttgcccc agcgagataa acggcaggtt gaaggtggcg
    17941 gtttcgcgtg tcacgttctc tggtcgcaca atggaaggcg cttcagtaat gtacgccagc
    18001 tcatttttat cccaggcgcg tttttcgcca aacgccagcc agtagttttt atcattacgc
    18061 gccatcagaa tcgggaaggc cgcgttggag atattcacca ccgcctgacc gcgcgcatta
    18121 cccgtgctgc catagaagtt agtagagtca tggaatacat ggaacttgct gacagatttg
    18181 tagttcgtat ccacgcccca cagcttgttg ataacgccct gaatctggcc gtcattaaca
    18241 ttgcgcgtcg tcagcgagaa ccagcgagcc tcgttacaac cgtcggtttt cgcacaaatc
    18301 gcggtatcga tctctttggc ctgacccgtc ttaaactgct cgataaattc gtttggcaga
    18361 ttaacaactt gctcaccttc atccagcgtc gcaccgttgg ataacgagag attgataatc
    18421 tcgttgatca cgttgggata ttcggcaaag accttgcgta catcatccgg aacaacacgg
    18481 gtattatttt gcccggtcgt cgaatagcga tgaataagct gatcgatatt cgccccgcga
    18541 acttcatcac ccagttcagt cagcgcaatg gtcgacttat tgccgcgcac tgaacccagt
    18601 tcaaaggtat cgataccaaa ggagatggtt tcgccccagc taaaagaaaa ttcaccattt
    18661 tcccctgtca cgccacggcc tgaattggtg tagtagttga cgccagcaac accatatccc
    18721 tggctatcca ccaggcgacc ttctgagaga atgatttccg aaggttgata ctgataaaac
    18781 tgttccgcgt tagccgacac gaaggaggcg ttcagatccg gttttgttcc cggagtcgtg
    18841 acggggacta ccggtgaagt atgagtggat ggcgctttat cagtcgcggc attgttttcc
    18901 acctcttcat tgaccagctt tttgaactct tccggtgcca gatcgatttg cttatacagc
    18961 gagtcgaaac gtttactctc gatcaccgag gagaactcca ggcacacctg ttctgtattc
    19021 gccgggcaac tgttcatgga ggtgaccagc gagagcgcat tgcttttctt gttgtcggaa
    19081 cccgccagtt cttgcgcgtc ctcaagacta aacgacactt tttcaaccgc acgcaggcta
    19141 cgcgcagctt ctgactgggt gtcgaaggta gcaattgtcg tgttgttccc tgccacacag
    19201 gtgactttgt cgcctggtgt aaaggtaaag ccatcgctgg attcatcatt acaggtgata
    19261 tcaccagtta cccgcaggct tccgcccagg gtcagataac ccgttttcgt aggaacaggt
    19321 tctggctccg gttctggtgt tggctcagga tcaggtgtcg gttccggcgt aggttctggg
    19381 tccggcgttg gctcaggcgt cggctccggg tttggtgttg gatcaggttt cacttccggc
    19441 aaagaccctg ttccagaatc tacaggcggc gtatcggagg aagatccgga gccaccgcca
    19501 tcacaaccgg ctaacagggt tgcactcaaa atagccgcta a
//
LOCUS       DAZGCS010000092        19023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGCS010000092 DAZGCS010000000
VERSION     DAZGCS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.092"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..617
                     /gene="yfiR"
                     /locus_tag="ACHK01_003571"
     CDS             99..617
                     /gene="yfiR"
                     /locus_tag="ACHK01_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO8098469.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            607..1833
                     /gene="dgcN"
                     /locus_tag="ACHK01_003572"
     CDS             607..1833
                     /gene="dgcN"
                     /locus_tag="ACHK01_003572"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO8098470.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1849..2331
                     /gene="yfiB"
                     /locus_tag="ACHK01_003573"
     CDS             1849..2331
                     /gene="yfiB"
                     /locus_tag="ACHK01_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8098471.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2408..2755)
                     /gene="rplS"
                     /locus_tag="ACHK01_003574"
     CDS             complement(2408..2755)
                     /gene="rplS"
                     /locus_tag="ACHK01_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO8098472.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2797..3564)
                     /gene="trmD"
                     /locus_tag="ACHK01_003575"
     CDS             complement(2797..3564)
                     /gene="trmD"
                     /locus_tag="ACHK01_003575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO8098473.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3595..4143)
                     /gene="rimM"
                     /locus_tag="ACHK01_003576"
     CDS             complement(3595..4143)
                     /gene="rimM"
                     /locus_tag="ACHK01_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO8098474.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4162..4410)
                     /gene="rpsP"
                     /locus_tag="ACHK01_003577"
     CDS             complement(4162..4410)
                     /gene="rpsP"
                     /locus_tag="ACHK01_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO8098475.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4547..5908)
                     /gene="ffh"
                     /locus_tag="ACHK01_003578"
     CDS             complement(4547..5908)
                     /gene="ffh"
                     /locus_tag="ACHK01_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO8098476.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6075..6866
                     /gene="ypjD"
                     /locus_tag="ACHK01_003579"
     CDS             6075..6866
                     /gene="ypjD"
                     /locus_tag="ACHK01_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO8098477.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6887..8173
                     /gene="yfjD"
                     /locus_tag="ACHK01_003580"
     CDS             6887..8173
                     /gene="yfjD"
                     /locus_tag="ACHK01_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO8098478.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            8296..8901
                     /locus_tag="ACHK01_003581"
     CDS             8296..8901
                     /locus_tag="ACHK01_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO8098479.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(8936..9526)
                     /gene="grpE"
                     /locus_tag="ACHK01_003582"
     CDS             complement(8936..9526)
                     /gene="grpE"
                     /locus_tag="ACHK01_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO8098480.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            9649..10527
                     /gene="nadK"
                     /locus_tag="ACHK01_003583"
     CDS             9649..10527
                     /gene="nadK"
                     /locus_tag="ACHK01_003583"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO8098481.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10613..12274
                     /gene="recN"
                     /locus_tag="ACHK01_003584"
     CDS             10613..12274
                     /gene="recN"
                     /locus_tag="ACHK01_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO8098482.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12423..12764
                     /gene="bamE"
                     /locus_tag="ACHK01_003585"
     CDS             12423..12764
                     /gene="bamE"
                     /locus_tag="ACHK01_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO8098483.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12826..13116)
                     /gene="yfjF"
                     /locus_tag="ACHK01_003586"
     CDS             complement(12826..13116)
                     /gene="yfjF"
                     /locus_tag="ACHK01_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO8098484.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(13106..13543)
                     /gene="ratA"
                     /locus_tag="ACHK01_003587"
     CDS             complement(13106..13543)
                     /gene="ratA"
                     /locus_tag="ACHK01_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO8098485.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13714..14196
                     /gene="smpB"
                     /locus_tag="ACHK01_003588"
     CDS             13714..14196
                     /gene="smpB"
                     /locus_tag="ACHK01_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO8098486.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14411..14773
                     /gene="ssrA"
                     /locus_tag="ACHK01_003589"
     tmRNA           14411..14773
                     /gene="ssrA"
                     /locus_tag="ACHK01_003589"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            15008..16216
                     /locus_tag="ACHK01_003590"
     CDS             15008..16216
                     /locus_tag="ACHK01_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFO8098487.1"
                     /translation="MSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNI
                     VEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRWGR
                     GSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTLIS
                     GPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLID
                     RRRVEYDIFLLVEELHVIDIIKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLF
                     IEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQILN
                     EADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFIT
                     ELIELYAELS"
     gene            complement(16251..17684)
                     /locus_tag="ACHK01_003591"
     CDS             complement(16251..17684)
                     /locus_tag="ACHK01_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO8098488.1"
                     /translation="MSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAE
                     LLRKVGKNEWSRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLF
                     AGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTD
                     VLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDV
                     ARIIRAKKPAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIID
                     GKHFLPQHRERIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILT
                     PKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMA
                     TGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVP
                     VFEAVARLLEPYILKAVSADAGKTGQP"
     gene            18091..18864
                     /locus_tag="ACHK01_003592"
     CDS             18091..18864
                     /locus_tag="ACHK01_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098489.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETIIFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(18922..>19023)
                     /locus_tag="ACHK01_003593"
     CDS             complement(18922..>19023)
                     /locus_tag="ACHK01_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8098490.1"
                     /translation="GRLIASGVPRQKVAIIYDVGISTLYKKFSVGDK"
BASE COUNT         4988 a         4833 c         4350 g         4852 t
ORIGIN      
        1 ttctcacatt gatgacgatc gcatgaaaaa atgatatttt aatgagcatt ctgaaaaaat
       61 atcgatgcat ttcgagcgaa gatggtgggg atccctgaat gcgtttttct caccgactgt
      121 tcttacttct gattctcctg ctgacgggag caccaatact cgctcaggag ccttctgacg
      181 ttgcgaaaaa cgtgcgtatg atggtttccg gtattgtcag ttacacccgc tggcctgcgc
      241 tatcagggcc accaaagtta tgtatctttt catcttcacg ttttagtacc gccctacaag
      301 agaatgccgc gacatctttg ccttatctgc ctgtcatcat tcacactcaa caagaggcga
      361 tgatttcagg ctgcaatggt ttttattttg gcaacgagtc accgacattc cagatggaat
      421 taaccgaaca atatccgtca aaggcgttat tattaattgc cgaacaaaat accgagtgca
      481 ttattggtag cgccttttgt ctgatcatcc acaataatga cgtcagattt gccgtaaacc
      541 tggatgcctt atcgcgtagc ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga
      601 agaaaaatga tggataacga taattctctt aataagcgcc ccacgtttaa aagagcatta
      661 cgcaacatca gtatgaccag catatttatc actatgatgc tgatctggtt gctgctttcc
      721 gtgacctcgg tgctgaccct gaaacagtac gcgcaaaaaa acctggcact gacagcagca
      781 acaatgactt acagtctgga agcagctgtc gtttttgccg atggccctgc agcaactgaa
      841 acactggcag cgctgggcca gcaagggcaa ttttcaactg cagaagtacg tgataagcag
      901 caaaatattc tggcgtcctg gcattacacc cgtaaggatc caggcgatac tttcagcaat
      961 ttcataagcc actggctctt ccccgccccc atcattcagc cgattcgtca caatggtgaa
     1021 accattggcg aagtacgctt aaccgctcgc gacagttcaa tcagccattt tatctggttt
     1081 tcgctcgccg tactgaccgg ttgtattctg ctggcatcag gcatcgcaat taccctcacc
     1141 cgccatttgc acaatggcct ggtagaagcg ctgaaaaata tcaccgatgt cgtacatgat
     1201 gtgcgttcca accgcaattt ttcccgacga gtttcggaag aacgtatcgc tgagtttcac
     1261 cgcttcgctc tcgacttcaa cagtctgctg gatgaaatgg aagagtggca gcttcgttta
     1321 caggccaaaa atgcgcagct tctgcgtacc gcgctacatg acccattaac cgggctggct
     1381 aaccgcgcag cgtttcgtag cggcatcaat acgttgatga acaattccga tgcccgaaaa
     1441 acgtcggcgt tactatttct tgatggcgat aatttcaaat atatcaatga tacctggggt
     1501 catgcgacgg gcgatagagt cttgattgaa atcgcaaaac ggttagctga atttggcggg
     1561 ctgcgacata aagcataccg cctgggcggc gatgaattcg ctatggtgct ctatgatgta
     1621 cagtcggaat ctgaagtgca gcagatatgc tcagcactga cacaaatctt taatctccct
     1681 tttgatcttc ataatggcca tcagaccacc atgacattaa gcattggtta cgcgatgacc
     1741 attgagcacg cctctgcgga aaaattacaa gagcttgccg atcacaatat gtatcaggcc
     1801 aaacaccagc gtgccgaaaa gctggtgaga taacaaggat atatcgatat gataaagcac
     1861 ctggtagcac ccctgatttt cacctcacta atactgactg gctgccagtc ccctcaggga
     1921 aagtttactc ctgagcaagt cgccgctatg caatcttatg gatttactga atccgccggc
     1981 gactggtcgc tgggcttatc agatgccatt ctgttcgcaa aaaatgacta caaattgctc
     2041 ccggaaagcc agcaacagat ccaaaccatg gcagccaaat tggcctcgac agggctaaca
     2101 catgcccgta tggatggaca caccgataac tatggtgaag acagttacaa cgaaggctta
     2161 tcattgaaac gggcgaatgt cgtggccgat gcatgggcta tgggtggaca aattccacgc
     2221 agcaatctca ccacacaggg tttaggaaaa aaatatccca tagccagtaa caagaccgcc
     2281 cagggccgcg ccgagaaccg ccgcgtcgca gtggtgatta caacccctta aaacacgagc
     2341 aaacagacaa aaaaagggcc agccaattgg ccagcccttc ttaacaggat gtcgcttaag
     2401 cgaaatctta gttaagacgc tctttgatac gagcagcctt accagtacgc tcacgcaggt
     2461 agtacagttt agctttacga acagcaccac gacgtttgac agaaatgctg tcaactaccg
     2521 gagagtgagt ctggaagaca cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga
     2581 atgcagagtg cagaccgcgg ttacgaatag cgataaccac gccctcgaat gcctgcagac
     2641 gttttttgga accttcaaca acccatactt tcacttccac ggtatcaccc ggacggaagg
     2701 aaggtacgtc ctgcttcatc tgctcttgtt caagttgctt aataatgttg ctcataattt
     2761 aatctcttat cctgggtaaa ctgatatctc gggggcttac gccatcccat catgtttatg
     2821 ttgctgttgt gcgtgttccg ttttgaactc cgccagcaac cttgcttgct cttcagtcag
     2881 agccaggttt tccagaagtt caggtcttct aagccaggta cggcccagcg actgtttcaa
     2941 acgccagcga cgtatctcgg catggttgcc cgacagtaac actggcggaa cttccatccc
     3001 ttctaacacc tcaggccgcg tatagtgcgg gcaatccagc aatccttcag caaaggaatc
     3061 ttccgttgcc gaggcttcat gtcccagtac tcccggaata aaccgggaaa cggagtcaat
     3121 cagcgtcatt gctggtaact caccaccact gagaacgtaa tcgccgattg accattcttc
     3181 gtcaatttcg gtttggatca cgcgctcatc tataccttcg tagcgaccgc acaccagaat
     3241 caatttttga ttcgttgcca gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg
     3301 tgacagataa atcacctttg cgccttcacc cgccgcggct tttgctgcat gaatggcgtc
     3361 ccgcaagggt tgcaccatca ttaacatccc cggtccgccg ccgtaaggac gatcgtccac
     3421 ggtacggtgc cggtcatgcg tgaagtcgcg aggactccaa ctctggatgc tcagcaggcc
     3481 atttttaact gcccggccag ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa
     3541 caggctaatt atgccaatcc acatagcgcc gtcttttacc gtttatccgg tggtttaaaa
     3601 accaggatcc caatctactt cgattgaacg agtagtgaga tcgactttct tgataacctg
     3661 cccatcgagg aacggtacga gacgttcctt gataccaaac gcatctttca ggtttgcctt
     3721 aatgacgaga acgtcattag atccggtttc catcatatcg acgactttac cgagatcgta
     3781 gccttcagtg gttactacct ggcagcccat caggtctttc cagtagtagt caccctcttc
     3841 aagctgaggc agctgcgatg aatccacgac aatttcacaa ttcgtcagca ggttcgccgc
     3901 atcacgatcg tcaacgcctt tcagcttgat gatcatgtcc tgattgtggt gcttccagct
     3961 ttccagctgg acttgctgcc actgacccgc cttctggata aaccagggct gatagtcaaa
     4021 aatgctttcg gcgtcttcgg tggaagaaaa cactctgagc cacccacgaa taccgtaaga
     4081 cgaacccatt tttcccaaaa cgatgggatc aacaggtgct tgcgcggtga gttgtttgct
     4141 catcatgacc accgtgacag attaagctgc tttgtttact tctttgatca gcgcagcaac
     4201 gcgatcagaa atagttgcgc cctggccaac ccagtgagcg atgcgatcca gatccaggcg
     4261 agtgccttct tctttttcgc tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg
     4321 accgttgcgt gcattacggc tgtcagcgac aacaacctgg tagaacggac gctttttagc
     4381 gccgtgacgt gctaaacgaa tagttaccat aacatcctct tgtgtgaata aaacaaccgg
     4441 accccatcga ggaacggagt ccggtgtcat attaaaagcc cgaaaatttt actgatttct
     4501 ggagaaaatg caatcagcag ttaataactc tgtcgcataa ggtcatttag cgaccaggaa
     4561 atcccggcgg catcatcccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
     4621 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
     4681 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
     4741 tagcgcgctc tttcatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
     4801 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
     4861 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
     4921 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
     4981 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
     5041 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
     5101 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
     5161 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
     5221 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
     5281 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
     5341 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
     5401 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
     5461 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
     5521 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
     5581 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
     5641 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
     5701 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
     5761 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
     5821 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
     5881 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
     5941 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
     6001 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
     6061 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
     6121 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
     6181 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
     6241 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
     6301 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
     6361 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
     6421 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
     6481 ccacgctaat tatcgccgcc ctgtacgcgc tgcaactggc gtggattgat taccaactga
     6541 agaacaagaa gctggcattt aaccaggaaa tgccgccatt gatgagtatc gagcgtaaaa
     6601 tgttccacat cacgcagatt ggcgtggtgc tgctaacgct cacgctttgc actggcctgt
     6661 tctacatgca caacctattt agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg
     6721 cgtggtttgt ctatattgtg ctgctgtggg gacattacca tgaaggatgg cgtggacgcc
     6781 gcgtcgtctg gtttaacgtt gcgggcgcgg taattctgac actggcctac ttcggcagcc
     6841 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
     6901 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
     6961 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
     7021 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
     7081 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
     7141 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
     7201 actggtattt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
     7261 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
     7321 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
     7381 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
     7441 ttcccgtcgc aatcaggata tgctgctgtc ggtgctcgat ctggaaaaaa tgaccgttga
     7501 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
     7561 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
     7621 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
     7681 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
     7741 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
     7801 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
     7861 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
     7921 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
     7981 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
     8041 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
     8101 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
     8161 tgtggcggag taacgcaatg gcgaactccg gttcgccttt tttttatcgc cgtcttttag
     8221 gattccatca attcctgtca tttacttagc aatgtaataa tgtcttttca agcattcgga
     8281 aaggatattg atgttatgtc agaagattat gtaattgaat gggataagga ttttgcagac
     8341 gatctcaacg tcgtcgccag cgttttttta cagcacaccc caaccttatg gcccactatt
     8401 ttctctcaac tttcgacgca acctgaaatc tttgaagacg aagatgaaga cgaatatgga
     8461 ttgcaggacg ttctcgattg cagtggtggc gatctcggga atcgtgacct ggcgcaggca
     8521 tttttacagg ttctacgcgg tgaaggatta attcaactcg ttgactggaa aggtgaagat
     8581 gaagaaggtg agctggctaa ttttgctgca gatcgctttt atgagctcac aaaaaacctg
     8641 atagcttctg aggaattaag gagtcttctt gtcgaaataa cccaagagga tgaaatctcc
     8701 gatgtctgtg aagccggaga ccgctacctt gacgaaatct ttgaacgcat ccaaaccgaa
     8761 ctcaataaaa ggggctttca gatttttgat ctgaatgaag gatctgacac ctataacgtg
     8821 gtcgttctgc caatgaacga atataaaaaa atagatgatt tcaacacgcc gtggctggaa
     8881 gtacaggatt ttttaagcta aaaaaatggc cgacttcgcg tcggccatca gatatttact
     8941 tcgccttcgc tacagtaacc atcgccgcac gaatcgtacg accattcagc gtataaccct
     9001 tctgcataat gcccagtacg ttacctggcg caacgtcatc agattccacc attgcgatag
     9061 cctgatgcac attcgggtcc agcggcacgt tagtttcggc gatcacttca acaccaaact
     9121 tacgcacaac atccagcatc gacttcagcg tcagctcaat gccttcaacc atcgcagaca
     9181 tatccgggtt agctttatca gccacttcca gcgcacgatc caggctatca atcaccggca
     9241 gcaattcgtt gatgaatttc tccagcgcga atttgtgggc tttttcaata tccagttcag
     9301 tacgtcgacg caggttttcc atttcggctt ttacacgcaa aatgccgtca cgttcacggg
     9361 tctgggcttc agccagctga gcttcgagat tcgcaatttt ttcatcgcgc ggatccacct
     9421 gctcagcaga agcttctggc tcaactgcct caatctcttc gtgctgatcc atgataattt
     9481 cttccggggc ttgcccctca ggcgttttct gttctttact actcatgaat ttctccgcgt
     9541 ttttttcgca ttcatctcgc taacttcgct tattatgggg atcagtttca gggtttcaag
     9601 ggaagcactc acattgtcat caatcttcgc aacaaggacc tcggaaaaat gaataatcat
     9661 ttcaagtgta ttggcattgt gggacaccca cggcacccca ctgcactgac aacacatgaa
     9721 atgctctacc gctggctgtg cacaaaaggt tacgaggtca tcgttgagca acaaatcgct
     9781 cacgaactgc aactgaagaa tgtgaaaact ggcacgctcg cggagattgg gcaactagct
     9841 gatctcgcgg tagtcgttgg tggcgacggt aatatgctgg gcgcggcacg cacactcgcc
     9901 cgttacgata ttaaagttat tggaatcaac cgtggcaacc tgggtttcct gactgacctt
     9961 gaccccgata acgcccagca acagttagcc gatgtgctgg aaggccacta catcagcgag
    10021 aaacgttttt tgctggaagc gcaagtctgt cagcaagatt gtcagaaacg catcagcacc
    10081 gcgataaatg aagtggtgct tcatccaggc aaagtggcgc atatgattga gttcgaagtg
    10141 tatatcgacg agatctttgc gttttctcag cgatctgatg gactaattat ttcgacgcca
    10201 acaggctcca ccgcctattc cctctctgca ggcggtccta ttctgacccc ctctctggat
    10261 gcgattaccc tggtgcccat gttcccgcat acgttgtcag cacgaccact ggtcataaac
    10321 agcagcagca cgatccgtct gcgtttttcg catcgccgta acgacctaga aatcagttgc
    10381 gacagccaga tagcactgcc gattcaggaa ggtgaagatg tcctgattcg tcgctgtgat
    10441 taccatctga atctgattca tccgaaagat tacagttatt tcaacacatt aagcaccaag
    10501 ctcggctggt caaaaaaatt attctaattt tacgccagcc tctttactgt atataaaacc
    10561 agtttatact gtacacaata acagtaatgg tttttcatac aggaaaacga ctatgttggc
    10621 acaactgacc atcagcaact ttgctatcgt tcgtgagctt gagattgatt ttcatagcgg
    10681 catgaccgta ataactggcg agaccggcgc gggtaaatct attgcaatag atgccctcgg
    10741 tctttgtctc ggtggtcgcg ctgaagccga catggtgcgt accggcgctg ctcgcgctga
    10801 cctgtgcgcc cgtttttctc tgaaagatac gccagcggct ctgcgctggc tggaagaaaa
    10861 ccagcttgaa gacgggcatg aatgtttgct tcgtcgcgtg atcagcagcg atggtcgctc
    10921 ccgtggtttc atcaacggta cagctgttcc tctgtcacaa ctgcgcgaac tgggtcagtt
    10981 gctgattcag atccatggtc agcacgctca tcagttactc accaaacccg agcaccaaaa
    11041 gttcctgctt gatggctatg ccaatgaaac ctctctactc caggaaatga cagcacgtta
    11101 tcagttgtgg catcaaagct gccgtgacct cgcgcatcat caacagttaa gtcaggaacg
    11161 cgctgcccgt gcggaactgc tgcaatacca attaaaagaa cttaacgaat ttaatccgca
    11221 gcccggagag tttgaacaaa tcgacgaaga gtacaaacgt ctggcgaaca gcggtcaatt
    11281 gctgaccacc agccagaatg cattggcatt aatggccgac ggtgaagacg caaacctgca
    11341 aagtcagctt tacacggcta aacaactggt gagcgaattg attggcatgg acagcaaact
    11401 gtccggcgta cttgatatgc tggaagaagc taccatccag attgctgaag ccagcgatga
    11461 actgcgccac tactgcgatc gtctggatct cgatcccaac cgactatttg aacttgaaca
    11521 gcgcatctca aaacagattt cgctggcacg taaacatcac gtcagccctg aggcattgcc
    11581 acagtattac cagtcgctac tggaagaaca gcagcaactg gacgatcagg ccgactcaca
    11641 agaaacgctt gcgctggcgg taacgaaaca tcatcagcag gcactggaaa tcgcgcgcgc
    11701 attacaccaa caacgccagc aatatgcaga agaacttgca cagctgatca ccgacagtat
    11761 gcatgcgctc tcaatgccgc atgggcagtt tacgatcgat gttaaatttg acgagcatca
    11821 cctgggcgct gacggtgccg atcgtattga gtttcgggta accaccaacc caggtcagcc
    11881 aatgcagcct attgccaaag tcgcatccgg tggtgaattg tcccgcatcg cactggcaat
    11941 ccaggtcatc acggcgcgta aaatggaaac cccggcactg atttttgatg aagtggatgt
    12001 agggattagc ggtccaacag cggcagttgt cggcaaactg ctgcgtcaac tcggcgaatc
    12061 aactcaggtg atgtgtgtta cccacctgcc acaagtcgcg ggatgtggtc atcaacacta
    12121 ttttgtcagc aaagaaaccg atggtgcgat gacagaaacg catatgcaat ccctgaataa
    12181 aaaagcgcgg ttacaagagc tggcgcgcct gcttggtggc agtgaagtca cacgtaatac
    12241 actggcgaat gcgaaagaac tgcttgcagc gtaaactttt ttcctgcttc acggtcagag
    12301 taaacagcaa aacgccgtaa gaccggaaag caaaaggttt taaagtgatg aaaggtctat
    12361 tatcatcggc atattacaga tgagccacgt actgctcggg cccgaaaagg aatcaaatca
    12421 ctatgcgctg taaaacgctg actgctgcag cagcagtact attgatgttg accgcaggct
    12481 gttccactct ggagcgagtg gtttaccgtc ctgacatcaa ccaggggaac tatctgaccg
    12541 ctaacgacgt atccaaaata cgtgttggca tgacgcaaca acaagttgcg tacgcattgg
    12601 gtacaccgct gatgtccgat ccatttggta cgaatacctg gttctatgtc ttccgccagc
    12661 aaccaggtca tgaaggtgta acgcagcaaa cgctgacgct gacctttaac agtagcggtg
    12721 tgttgaccaa tattgataac aaacctgcgc tgagtggtaa ctaataataa agttgctctc
    12781 aaagacgtta aaaaaggtgc tcaatgagca ccttttttct gtctgttatt tattcgctga
    12841 tttttctgct cgttgcctgc gaagctcttt cggatcggca atgagagggc gataaatctc
    12901 cacccgatcg ccatcatgca cactatcgct tagttttgcc ggacggctgt aaatgccgac
    12961 tttgttttta gttaaatcga tatcggtacg caattccagc aagccactgg cgcgaatagc
    13021 ttcttcaacc gtcgcgccct cctgcagcgt cacgcgctgc aggtactgct tctcaggtag
    13081 cgcataagcc acctcaacgg caattttacc tggcactgta aacctctttc gcacgaaccg
    13141 taaaagcctg gaccatatta gacgccagct ctttaaacac gcgaccaaag gcgagttcaa
    13201 tcaacttatt ggtaaactca aagtcgagat gaaactcgat acgacacgcc tcctggctca
    13261 gcggcgtaaa cttccatcca ccaatcaatt tcttgaacgg cccatccacc agattcataa
    13321 gaatactttg gttactggtc aactggttgc gggtagtaaa cgttttgctg atcccagcct
    13381 tagagacatc taccgccgca gtcatctgcc caggagtgga ctccagaatc cgacttccgg
    13441 tacaacccgg caaaaactga ggataagact gaacgtcatt cactaactga tacatttgct
    13501 ccgcgctgta gggtaccagt gcggtccggc taatctgagg cataacaatt tccatcaaca
    13561 aaaatccaac aaataatatc atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg
    13621 taacatcggg ttcatgctaa gatagagcct tgtcccccgc aggattgata tggggtgttt
    13681 tcgatttcag attaccgatg attcacgacg cttatgacga agaaaaaagc acataaacct
    13741 ggttcagcga ccatcgcgct taacaagcgc gcccgtcacg aatactttat cgaagaagag
    13801 ttcgaagcgg gacttgccct gcaaggctgg gaggttaaat ccctgcgcgc aggaaaagcc
    13861 aatatcagcg acagctacgt ccttctgcgt gacggagagg catttctgtt tggcgctaac
    13921 atcacgccaa tggccgtggc ctccacgcat gtggtgtgcg atcctacccg tacccgcaag
    13981 ttacttctca accaacgcga actggactca ttgtacggtc gcgtcaatcg agaaggctat
    14041 accgtagtgg cgctctccct gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc
    14101 gccaaaggta aaaaacagca cgataaacgt tcagatatca aagaacgcga atggcaggtg
    14161 gataaagcac gtatcatgaa aaacgcccac cgttaaacct gcactccaat tattgaccag
    14221 ttcctcaccg cgcctccctc tccggcggcg cgaatgaaca tcttattggc tatcacatcc
    14281 gacacaaatg ttgccatccc attgcttaat cgaataaaaa tcaggctaca tgggtgctaa
    14341 atctttaacg ataacgccat tgaggctggt catggcgctc ataaatctgg tatacttacc
    14401 tttacacatt ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg
    14461 aggggcggtt ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct
    14521 ttagcagctt aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg
    14581 atcaagagag gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt
    14641 aaaacttaat caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta
    14701 aagactgact aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc
    14761 gccagctcca ccaatcatga ttggacggtg taaggacaac accaacaaaa acaggaagtt
    14821 agcagtctca gcaggacacc gaccagacgg tgaggagata aaaaaggata cgcaaaggag
    14881 ccgtggctct cgagtgacac aaaagcccgc ttatgcgggc tttttgtttt tcccttaagt
    14941 tcctagcctc ttatctataa ctatggaaaa atgttaaccc tgactgtatg ttaacaaagg
    15001 gatgtatatg tcggtttttc ataactggct gcttgatatc gcaagcggga attactttat
    15061 ctacatcaaa cgcctttctg caaacgacac aggcgcaaca ggtggccatc aggtcggact
    15121 ttatatcccc tcaaatatcg ttgaaaagct ttttccatct atcaatcata ctcgcgaact
    15181 gaacccttca gtcttcctta ctgcgcatgt atcatcccat gattgccctg atacccaagc
    15241 acgcgcaatt tattacaaca accgttattt tggtaagacc cgaaacgaaa aaagaattac
    15301 gcgctggggg agaggaagtc cattacagaa ccctgaaaat acaggagccc tcacaattct
    15361 tgctttcagg ttaaacgaac agaacactga ctgttccgag gtagatatat gggtctgcgt
    15421 caatcccgat gaagaggata tcatcgagtc tgctattggc gaaatcatac ctggaaccct
    15481 aatttccggc cctgccggac aaattttggg cggattgtct cttcagcaaa ctccagtaaa
    15541 tcataaatat gttattcctg aagactggaa gaagcgtttt ccttctggaa acgaaattat
    15601 tcaatatgct gctggccatt atgctaaaaa ctccaaggat ccagatgagc aactgattga
    15661 ccgtcggcgt gtcgagtatg atatttttct actcgtcgag gaattacatg ttattgatat
    15721 tattaagaaa ggattcgatt ctgtagatga gtttattgca ttagccaact ctgtcagtaa
    15781 tcgacgtaaa tcaagggcag gcaaatcact tgaacttcac ctagagaagc tttttatcga
    15841 gcacggacta cggcatttct ccactcaggc agttactgaa ggtaataaaa aaccagattt
    15901 cctgtttcct tcagcagagg catatcataa cgttgaattt cctgtagaaa acttacgtat
    15961 gctggcagta aagaccacct gcaaagatcg ctggcgtcag atactgaatg aagcagataa
    16021 aatccatcag gtacatttat ttacactgca agaaggtgtt tcttcagcac aataccgaga
    16081 aatgaaagat gcgggtgtca gactcgttgt accatcaact ttacataaaa aatacccaga
    16141 agcagttaga gaagaattaa taacgctcgg agcattcatt actgagctga tagagcttta
    16201 cgctgaacta tcataggctg actcccggct taaaaggccg ggagatgttc tcaaggctgc
    16261 ccagtcttac cagcatcagc agaaacagct ttgaggatat agggttccag aagtctggca
    16321 acagcttcaa atactggcac cacaactgag ttaccgaact gccgatatga ctgagtatct
    16381 gaaaccggaa tacggaatgg tttccctcca ggtttttcaa atcccataag gcgtgcgcac
    16441 tctcggggag tcagcctgcg tggtcgacga gcctgattac tctcattcat aaagtcagcc
    16501 tctcccgttg ccatatccca accacgatca ataagaattt ctgatccgtc tttgtgatat
    16561 ctggcagaaa gtgtgcgtgc aatgctttcc ttattctcag gattgaccaa cccaaagcca
    16621 aaaccattcc ccttggctgc atgctttttg gcgtagttat aaaggtactc ccatagtttt
    16681 ggcgtaagta tatatttact gtcgactaca ggctccagca attcaccaaa tgatgggcgg
    16741 tgttccggat aaaaacgact gatatcacgc aaggtaaacc ccttgtgaat attcagatct
    16801 cgtctaaatc cgaccagaac aatgcgttca cgatgctgag gcaaaaaatg cttcccatcg
    16861 ataatctttg gatcgttttt tcccatttct gcagcatcgg caacttcgta gcccagctcg
    16921 tcaagggtct ccataatgac tttgaaagtt ttacccttat catggctctt cagatttttg
    16981 acattttcca gcacaaaaat tgccggtttt tttgctcgta taatacgtgc cacgtcaaaa
    17041 aaaagtgttc cttgtgcttc acattcaaaa ccatgcgcac gaccaagtga gtttttctta
    17101 ctaacaccag caaggctaaa tggctggcac gggaaccccg caagaagcac atcatgatcc
    17161 ggcacatgct catcaatata tgcataagca tccgtttcca atacatcggt tttatcactc
    17221 agcgtgactt cccgaatatc gagattgaat ttatgcacct gttcatcgtt aaaccagttg
    17281 gccttgtatg tacgcacagc atctttattc cattcactgg taaaaacaca ctggcctcca
    17341 atggcctcaa aacctttccg tatccctcca attccagcaa ataagtcaat gaagcggaaa
    17401 gcatattccg gatggtttgc aggtggttct ggtagcatct tacgcagaag agactcttct
    17461 actgaagtca acgattttgg taaacacttg ccattaatcc agcggttaat ggtttcacgg
    17521 ctccactcat tttttccgac ttttctcagt aattcagcca catacttctg atcatagatt
    17581 tccagcactt tctcgataag ctttttatca ttttcctgtc gcagcttttc ttccgcctcg
    17641 gcttccttca gcagatgctg tgccaacact tcaaattcag acataattcc tccaaggggt
    17701 ctaatgggtg aaactttatc actcattcaa cccagaagga aatgttttat ctggatattt
    17761 aaacagtgac tacaacgtaa tctagcactg gtgatgcttt gttaggcata gagaatcatt
    17821 ctatatacga ctaatgacag aaaaacagca gacaagtagt ttgttcataa attaacgcat
    17881 actatgtgtc tacggttttc gagaccggtc caatcatcaa acgaaacata aaattagctc
    17941 acattatgag gaaaagtatc ttttttgtac tatgtaaatt caaaggctta gcctcatttc
    18001 tccgatggtt ttctcaacac tactggttgt gagcccttgc aatgatcatt aatatacgtc
    18061 tcacaaataa ttcttcatag atattgcaaa atggatatta ctgagtttcc ttctggagta
    18121 attgaacacc ttggctggta tgtataccga ttgattgatc cgagggacgg aagcaccttc
    18181 tatgtaggga aaggcaaagg taaccgcgta tttgcccata tgcgcggtga agtggcagcg
    18241 actgatgatg acgagttact gagcaacaag ctaaagcaaa ttagagaaat aaggttagca
    18301 ggacttgaag ttatccatgt catccatcga cacggaatga ctgatgaaaa gacggcgtac
    18361 gaagttgaag ctgcacttat tgatgcctac cctgggttaa cgaatatcat gaatggtgct
    18421 ggcagcaatg aattcggcgc cgcgcatgtc aaagagttga tagcaacata tcaacccgaa
    18481 accataatat ttcatcataa agcattaatg atttccgtta acagaagtgc aaaggattca
    18541 gagctttatg atgcggttcg atttagctgg cgcattaatg tctctcgcgc cagccaagca
    18601 gaagtcattc ttgctactgt aagggggatc gttcgagggg ttttcattgc tgataaatgg
    18661 ctcaaatcaa cacgtgaaaa tttccctacg atgaaatact gggacgagga tcctgacttt
    18721 gaggcaacac aaagttctcg ctatggtttt gaaggtcgag aagccccacc tgaaatagca
    18781 aatctttatc ttggaaaaaa aataccagat gaattaagaa aaaaaggagc tatgtccccg
    18841 gtccgttact cacctaattt ttgagtcttt aagtgataag cataaaccgc agcacgatct
    18901 tgcatacgac gtgctacggt ttcatttatc tccgaccgaa aacttcttat acagtgtcga
    18961 tataccaaca tcatagatga tcgccacctt ctggcgagga acgcctgatg caattaatcg
    19021 ccc
//
LOCUS       DAZGCS010000093        18537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGCS010000093 DAZGCS010000000
VERSION     DAZGCS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.093"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..590)
                     /gene="rsmG"
                     /locus_tag="ACHK01_003594"
     CDS             complement(<1..590)
                     /gene="rsmG"
                     /locus_tag="ACHK01_003594"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO8098491.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGER"
     gene            complement(654..2543)
                     /gene="mnmG"
                     /locus_tag="ACHK01_003595"
     CDS             complement(654..2543)
                     /gene="mnmG"
                     /locus_tag="ACHK01_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO8098492.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2922..3365)
                     /gene="mioC"
                     /locus_tag="ACHK01_003596"
     CDS             complement(2922..3365)
                     /gene="mioC"
                     /locus_tag="ACHK01_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO8098493.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3455..3913)
                     /gene="asnC"
                     /locus_tag="ACHK01_003597"
     CDS             complement(3455..3913)
                     /gene="asnC"
                     /locus_tag="ACHK01_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO8098494.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4065..5057
                     /gene="asnA"
                     /locus_tag="ACHK01_003598"
     CDS             4065..5057
                     /gene="asnA"
                     /locus_tag="ACHK01_003598"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO8098495.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5062..6513)
                     /gene="viaA"
                     /locus_tag="ACHK01_003599"
     CDS             complement(5062..6513)
                     /gene="viaA"
                     /locus_tag="ACHK01_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO8098496.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6507..8003)
                     /gene="ravA"
                     /locus_tag="ACHK01_003600"
     CDS             complement(6507..8003)
                     /gene="ravA"
                     /locus_tag="ACHK01_003600"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO8098497.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEIDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8226..10094
                     /gene="kup"
                     /locus_tag="ACHK01_003601"
     CDS             8226..10094
                     /gene="kup"
                     /locus_tag="ACHK01_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO8098498.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10261..10680
                     /gene="rbsD"
                     /locus_tag="ACHK01_003602"
     CDS             10261..10680
                     /gene="rbsD"
                     /locus_tag="ACHK01_003602"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO8098499.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10688..12193
                     /gene="rbsA"
                     /locus_tag="ACHK01_003603"
     CDS             10688..12193
                     /gene="rbsA"
                     /locus_tag="ACHK01_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO8098500.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12198..13163
                     /gene="rbsC"
                     /locus_tag="ACHK01_003604"
     CDS             12198..13163
                     /gene="rbsC"
                     /locus_tag="ACHK01_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO8098501.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13188..14078
                     /gene="rbsB"
                     /locus_tag="ACHK01_003605"
     CDS             13188..14078
                     /gene="rbsB"
                     /locus_tag="ACHK01_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO8098502.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14204..15133
                     /gene="rbsK"
                     /locus_tag="ACHK01_003606"
     CDS             14204..15133
                     /gene="rbsK"
                     /locus_tag="ACHK01_003606"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8098503.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALTPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15137..16129
                     /gene="rbsR"
                     /locus_tag="ACHK01_003607"
     CDS             15137..16129
                     /gene="rbsR"
                     /locus_tag="ACHK01_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO8098504.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16095..17522)
                     /gene="mdtD"
                     /locus_tag="ACHK01_003608"
     CDS             complement(16095..17522)
                     /gene="mdtD"
                     /locus_tag="ACHK01_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO8098505.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17545..18237)
                     /gene="yieP"
                     /locus_tag="ACHK01_003609"
     CDS             complement(17545..18237)
                     /gene="yieP"
                     /locus_tag="ACHK01_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO8098506.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4474 a         4598 c         4843 g         4622 t
ORIGIN      
        1 cgttcgccat ccagggcagg aacctgaagt ttaaccactg attcaacctg atattcttcg
       61 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
      121 tcaccaggaa gatggtgaca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
      181 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
      241 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
      301 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gccaggcagt
      361 ccaggaccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
      421 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
      481 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
      541 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
      601 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
      661 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
      721 gtgacgccag aaatacgtga agcctggccg atagaggccg gtttgtgatc gttaagtttg
      781 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc tggtagcagg
      841 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
      901 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
      961 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
     1021 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
     1081 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
     1141 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
     1201 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
     1261 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatag
     1321 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
     1381 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
     1441 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
     1501 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
     1561 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
     1621 taaatttcgt tagaagtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
     1681 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
     1741 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
     1801 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
     1861 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
     1921 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
     1981 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
     2041 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
     2101 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
     2161 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
     2221 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
     2281 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
     2341 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
     2401 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
     2461 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
     2521 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
     2581 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
     2641 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
     2701 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
     2761 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
     2821 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
     2881 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
     2941 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
     3001 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
     3061 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
     3121 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
     3181 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
     3241 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
     3301 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
     3361 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
     3421 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatagta cgcatgatcg
     3481 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
     3541 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
     3601 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
     3661 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
     3721 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
     3781 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
     3841 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
     3901 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
     3961 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
     4021 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
     4081 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
     4141 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
     4201 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
     4261 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
     4321 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagagcgtc
     4381 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
     4441 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
     4501 caaccgaagc tgcagttagc gaagagtttg gtttggcacc gttcctgccg gatcagatcc
     4561 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
     4621 agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagctga
     4681 gtgatggtca tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
     4741 agctgggcca tgcgggtctg aacggcgata ttctggtatg gaaccctgta ctggaagatg
     4801 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
     4861 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
     4921 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
     4981 aactgccgca tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg
     5041 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
     5101 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
     5161 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
     5221 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
     5281 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
     5341 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
     5401 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag
     5461 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
     5521 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
     5581 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
     5641 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
     5701 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
     5761 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
     5821 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
     5881 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
     5941 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
     6001 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg
     6061 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
     6121 acgctggaga aaaagcgtgt gtaacgctga agtgatcgtg ctgttagcat caaccaactg
     6181 tcgggcttgt tctgcccatg gagaattcag acgatgcagt aagtccagga tctgtggtag
     6241 ctgcacaata aactgtggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
     6301 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgctt cacgccagcg
     6361 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
     6421 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
     6481 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
     6541 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
     6601 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
     6661 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
     6721 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caatttccag attcagtttc
     6781 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
     6841 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
     6901 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
     6961 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
     7021 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
     7081 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
     7141 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
     7201 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
     7261 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
     7321 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
     7381 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
     7441 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
     7501 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
     7561 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
     7621 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
     7681 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
     7741 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
     7801 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
     7861 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
     7921 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
     7981 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
     8041 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt
     8101 ctttgagcca ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata
     8161 ctgtgcgctt ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact
     8221 agtctatgag cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag
     8281 ttgtctacgg cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc
     8341 agtttggttt tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc
     8401 tgctaatctt tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg
     8461 gtgagggggg gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca
     8521 catcaatgct ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca
     8581 taacacccgc tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc
     8641 tggatacctg gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa
     8701 aacatggcac ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga
     8761 ttctggcagg actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga
     8821 atccaatgtg ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag
     8881 gggcagtggt gttgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg
     8941 gtaagttccc tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt
     9001 acttcggtca gggagcgctg ttgttaaaga acccggaagc gattaagaac ccgttcttcc
     9061 tgttggcacc ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa
     9121 ttgcctcgca ggcggttatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg
     9181 gatatttgtc gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata
     9241 ttccgtttgt gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc
     9301 actccagcaa cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt
     9361 ctattctctc gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc
     9421 tgatcctgat tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac
     9481 tgctctccgg cggctggttg ccattgagcc tcggtactgt gatgtttatc gtgatgacca
     9541 cctggaagag cgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg
     9601 aagcgatgat tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtgt
     9661 atatgtcgcg tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca
     9721 aggtattgca tgagcgggtg attctgttaa ctctgcgtac cgaagacgcg ccatatgtcc
     9781 ataatgtccg tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa
     9841 gttatggttg gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag
     9901 gattaagttg ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg
     9961 gcaaacgccc gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg
    10021 cgctgcgtgc accagatcaa tttgaaattc cgccaaacag ggttatcgaa ctgggtactc
    10081 aggtcgaaat ctaacgccag acgcctcctt tctccataag ggggcgtttt tgttttcatg
    10141 gttaatcacc atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt
    10201 tcccaactca gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa
    10261 atgaaaaaag gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat
    10321 accgatacgc tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc
    10381 gatatggcat taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat
    10441 gaaatgcagg tcgaggctgc cattatcgcg gaagagatca aacaacataa tccgcaactc
    10501 cacgaaacgt tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa
    10561 attcgttaca ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt
    10621 cgcagcggag aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga
    10681 ggccgtcatg gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa
    10741 agccctctcg ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga
    10801 aaacggcgcg ggtaaatcca ccatgatgaa agtgcttact ggtatctatg ctcgcgatgc
    10861 cggcacgctt ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga
    10921 agccgggatt gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga
    10981 aaacattttc ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat
    11041 gtatgccgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct
    11101 ggtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
    11161 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
    11221 atccctgttc cgcgtcatac gcgagctgaa atcgcaaggc cgcggtattg tctatatctc
    11281 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgacgggca
    11341 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
    11401 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccgggag atatccgcct
    11461 gaaagtcgat aatctctgcg gacttggcgt taacgatgtc tcttttactt tacgcaaagg
    11521 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
    11581 ctacggcgca ctaccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
    11641 ccgttcaccg caggatggcc tggcaagcgg cattgtgtat atctccgaag accgtaaacg
    11701 tgacggttta gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
    11761 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
    11821 cattcgtctg tttaatgtga aaacaccgtc gatggaacag gcaattggtc tgctttccgg
    11881 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat
    11941 ccttgatgaa cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
    12001 taaccagttc aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt
    12061 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
    12121 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
    12181 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
    12241 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
    12301 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacctcgg
    12361 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt
    12421 ctgtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
    12481 tcaatgcgct ggtggctgtc gctgctgctc tcgcgttagg tgccgcaatt ggtgcggtaa
    12541 ccggggtgat tgtagcgaaa ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc
    12601 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
    12661 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
    12721 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
    12781 tggggcgtta catctacgcg ctgggcggca acgaagcagc aacgcgtctt tctggtatca
    12841 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
    12901 ggatcattga agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc
    12961 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
    13021 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
    13081 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
    13141 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
    13201 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
    13261 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
    13321 aagatggcgc gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc
    13381 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
    13441 ttctgctgat taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc
    13501 aggcgaacat cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
    13561 acattgcttc tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag
    13621 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg
    13681 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
    13741 agccagcaga ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgctc
    13801 atccggatgt tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg
    13861 cactgcaaac tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg
    13921 gcgaaaaagc ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga
    13981 ttggcgcgaa aggcgtcgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta
    14041 agtatccggt tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct
    14101 gatggtgaca taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac
    14161 gcgccaccct aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag
    14221 cctcgttgtt cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac
    14281 tccaggcgaa accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa
    14341 tcaggctgtg gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtacgggtga
    14401 tgacagcatt ggtgagagcg ttcgccagca gctcgccact gataacattg atattactcc
    14461 ggtcagcgtg atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg
    14521 tgagaatgtc atcggtattc atgccggcgc taatgctgcc cttaccccgg cgctggtgga
    14581 agcgcaacgt gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact
    14641 cgaaagtgtg atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa
    14701 cccggctccg gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc
    14761 aaacgaaacg gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc
    14821 gaaggcggcg caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag
    14881 tcgtggtgta tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca
    14941 ggctgtcgat accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct
    15001 ggaagaaaaa ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt
    15061 aacacgtaaa ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga
    15121 caggcagagg tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac
    15181 ctcaacagtt tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa
    15241 agttgaagcg gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa
    15301 actcaatcaa acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc
    15361 agaactggtg cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg
    15421 caataccgaa ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg
    15481 cgttgatggc ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca
    15541 acgttatccg acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga
    15601 tcttattcag gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa
    15661 aggtcatacc cgtatcgcct gtattaccgg tccgctggat aaaactccgg cgcgcctgcg
    15721 gttggaaggt tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga
    15781 agtcactggt gattttgaat ttaacggcgg gtttgacgcc atgcgccaac tgttatcaca
    15841 tccgctgcgt cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca
    15901 ggcgttatat caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga
    15961 tatcgaactg gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga
    16021 actgggggag ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca
    16081 acaacgatta caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt
    16141 cttttgatca aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca
    16201 acagtaataa tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct
    16261 tccatccctt cataaacgcg aaggacggcc gcacttacag caacgcctaa actaatcgac
    16321 agttgctgcg tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg
    16381 gcaagtgtga tggtattcat cgcggtaaat tgcgtcgaca tagccatccc taatataaac
    16441 aacggcaaga tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc
    16501 attagcccaa taatcaccgt gatccccact aacgtatggc gatagcccag acgacgtaag
    16561 acttgggtaa ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag
    16621 ccggcaataa acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt
    16681 acacaaccgg tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg
    16741 gttttaaata aatctaatga aattaatggg tttggcgtgt gtcgcgcatg gagaatatag
    16801 agaagcagta acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc
    16861 ctttccccga atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa
    16921 aagccagtga tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg
    16981 taaagaaggc ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc
    17041 caggtgacca gcacgccgcc aagaacgggg cctaaaattg gccccaccag acccggcatg
    17101 gcgacaaaat tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc
    17161 cgagcaacag gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc
    17221 tgtggtagcg aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca
    17281 agggtaaaaa tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc
    17341 atcgccaccg tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta
    17401 aggctatgag cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg
    17461 aagaaggcca tcgccgcgat ccacggcaaa cccgccatac tgcgcttctt tttatcgctc
    17521 attcaatgtc ctgttatcgg gttgtcactt atcaggtgag cgtagcagcg cctgacaagc
    17581 tttaaatgcc gcgtcgccat cgctttggac aatcgcatcg acaatcgcct ggtgcagatc
    17641 cagctttatc actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa
    17701 tagcgaggcg aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca
    17761 ggccatatcg acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc
    17821 ggccattaac gtattgagat gcgccttctg ttccgcggtg ccaaccgttg cagccagtag
    17881 gcaagcttgc ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa
    17941 gttctcttct gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg
    18001 tggcatgacc cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt
    18061 tttgaccgct tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc
    18121 accaggcaaa atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc
    18181 agccagaaca tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac
    18241 taatcttcct ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt
    18301 gcaaaaacgg caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc
    18361 gcggtcagaa aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg
    18421 ccaccactga cacggaacaa cggcaaacac gccgccgggt cagcggggtt ctcctgagaa
    18481 ttccgacaga gaaagcaaaa ataaatgctt gactctgtag cgggaaagcg tattatg
//
LOCUS       DAZGCS010000094        18427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGCS010000094 DAZGCS010000000
VERSION     DAZGCS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.094"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..1223
                     /gene="arnB"
                     /locus_tag="ACHK01_003610"
     CDS             84..1223
                     /gene="arnB"
                     /locus_tag="ACHK01_003610"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO8098507.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1227..2195
                     /gene="arnC"
                     /locus_tag="ACHK01_003611"
     CDS             1227..2195
                     /gene="arnC"
                     /locus_tag="ACHK01_003611"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO8098508.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2195..4177
                     /gene="arnA"
                     /locus_tag="ACHK01_003612"
     CDS             2195..4177
                     /gene="arnA"
                     /locus_tag="ACHK01_003612"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO8098509.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4174..5064
                     /gene="arnD"
                     /locus_tag="ACHK01_003613"
     CDS             4174..5064
                     /gene="arnD"
                     /locus_tag="ACHK01_003613"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO8098510.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5064..6716
                     /gene="arnT"
                     /locus_tag="ACHK01_003614"
     CDS             5064..6716
                     /gene="arnT"
                     /locus_tag="ACHK01_003614"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO8098511.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPQLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6713..7048
                     /gene="arnE"
                     /locus_tag="ACHK01_003615"
     CDS             6713..7048
                     /gene="arnE"
                     /locus_tag="ACHK01_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO8098512.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7048..7434
                     /gene="arnF"
                     /locus_tag="ACHK01_003616"
     CDS             7048..7434
                     /gene="arnF"
                     /locus_tag="ACHK01_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO8098513.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7428..7694)
                     /gene="pmrD"
                     /locus_tag="ACHK01_003617"
     CDS             complement(7428..7694)
                     /gene="pmrD"
                     /locus_tag="ACHK01_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO8098514.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7804..9159)
                     /gene="menE"
                     /locus_tag="ACHK01_003618"
     CDS             complement(7804..9159)
                     /gene="menE"
                     /locus_tag="ACHK01_003618"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO8098515.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9156..10118)
                     /gene="menC"
                     /locus_tag="ACHK01_003619"
     CDS             complement(9156..10118)
                     /gene="menC"
                     /locus_tag="ACHK01_003619"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO8098516.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10118..10975)
                     /gene="menB"
                     /locus_tag="ACHK01_003620"
     CDS             complement(10118..10975)
                     /gene="menB"
                     /locus_tag="ACHK01_003620"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO8098517.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10990..11748)
                     /gene="menH"
                     /locus_tag="ACHK01_003621"
     CDS             complement(10990..11748)
                     /gene="menH"
                     /locus_tag="ACHK01_003621"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO8098518.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11745..13415)
                     /gene="menD"
                     /locus_tag="ACHK01_003622"
     CDS             complement(11745..13415)
                     /gene="menD"
                     /locus_tag="ACHK01_003622"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO8098519.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13504..14799)
                     /gene="menF"
                     /locus_tag="ACHK01_003623"
     CDS             complement(13504..14799)
                     /gene="menF"
                     /locus_tag="ACHK01_003623"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO8098520.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14878..15183)
                     /gene="elaB"
                     /locus_tag="ACHK01_003624"
     CDS             complement(14878..15183)
                     /gene="elaB"
                     /locus_tag="ACHK01_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO8098521.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15238..15699)
                     /locus_tag="ACHK01_003625"
     CDS             complement(15238..15699)
                     /locus_tag="ACHK01_003625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8098522.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15764..16681
                     /gene="rbn"
                     /locus_tag="ACHK01_003626"
     CDS             15764..16681
                     /gene="rbn"
                     /locus_tag="ACHK01_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO8098523.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16869..18089
                     /gene="elaD"
                     /locus_tag="ACHK01_003627"
     CDS             16869..18089
                     /gene="elaD"
                     /locus_tag="ACHK01_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO8098524.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFPPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLTRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
BASE COUNT         4437 a         4900 c         4575 g         4515 t
ORIGIN      
        1 tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt
       61 attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg
      121 gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggctc acaaccggtc
      181 cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag catgccatcg
      241 cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa attggcaagg
      301 gcgatgaagt gattacgcct tccctgacct gggtttcaac cctcaatatg atttccttgt
      361 tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc acgcctgaag
      421 cgatcgagtc agccataacg ccacgcacta aagccatcat tccggtgcat tatgccggtg
      481 cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg
      541 aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg acatattggc gcaaaaggta
      601 ccgctatttt ttcatttcat gccattaaaa atattacctg tgcggaaggt ggcctgattg
      661 taactgataa tgaaaacctt gcccgccagc tacggatgct gaaatttcac ggtctgggtg
      721 tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg
      781 gctataagta caatctgacc gatattaacg ccgcaattgc cctgacacag ttagcaaaat
      841 tagagcacct caacacccgt cggcgcgaaa ttgcccagca atatcagcaa gcactggcag
      901 ctctcccctt tcagccatta agccttcccg cctggccgca cgttcacgcc tggcatctgt
      961 ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt
     1021 taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt
     1081 atcgcgagcg ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct
     1141 gttctttgcc gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc atcacagccc
     1201 ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg
     1261 tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacca
     1321 cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg
     1381 ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta
     1441 ttttgcttaa ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta
     1501 ccggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa gaaatccccc
     1561 gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc cagaaccgcc
     1621 aggacagctg gtttcgtaaa acagcttcga agatgattaa ccggcttatt cagcgcacca
     1681 ccggcaaagc gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt catattgtcg
     1741 atgcgatgtt gcactgccat gaacgtagca cctttatccc gattctggcg aatatcttcg
     1801 cccgccgtgc cattgaaatt ccagtacatc attccgagcg tgagtttggt gaatccaaat
     1861 acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc
     1921 cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt agcattgcgg
     1981 tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta
     2041 tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattactcg
     2101 gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc
     2161 aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca
     2221 cgatatggga tgcctcggta ttgaagctct gctggctgcc ggttacgaaa ttagcgccat
     2281 ttttacccat accgataatc ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc
     2341 agcggaaaga ggcattccgg tttatgcgcc ggatgacgtt aatcatccgc tgtgggtgga
     2401 acgcattgcc caactgtcgc cagatgtgat tttctctttt tattatcgcc atcttatttg
     2461 cgacgaaatt ttgcagctcg ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc
     2521 aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtc aacggtgaaa cggaaactgg
     2581 cgttacattg caccgaatgg tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg
     2641 cattgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc
     2701 gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaatcgc
     2761 ccagcgcgaa aacgaagcca cctgttttgg tcgcagaacg ccggatgaca gtttccttga
     2821 atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg atccgtggcc
     2881 gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc
     2941 tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc tgattgcctg
     3001 tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg
     3061 ctcgcaatta gcgcagacgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc
     3121 ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa
     3181 ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg
     3241 cagcgatgcg ataagccgtt ttctgaatca tccgcatttt cactttgttg aaggcgatat
     3301 cagtattcat tccgaatgga ttgagtatca tgtcaaaaaa tgtgatgtcg tcttgccgct
     3361 ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga
     3421 ttttgaagag aatctgcgca ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt
     3481 cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc
     3541 taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtat caaaacaatt
     3601 acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca ccctcttccg
     3661 cccgtttaac tggatgggac cgcgactgga taaccttaat gcggcacgaa tcggcagctc
     3721 ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccgatta agctgattga
     3781 tggcggaaaa caaaaacgct gctttactga tattcgcgat ggtatcgagg cgttataccg
     3841 cattatcgaa aatgcgggaa atcgctgcga cggtgaaatt atcaacattg gcaatcctga
     3901 gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc
     3961 gctgcgccat catttcccac cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta
     4021 cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccaccgctg
     4081 cctggactgg gagccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tggatttctt
     4141 cctgcgcacc gttgatctta cggataaacc atcatgacca aagtaggctt acgcattgat
     4201 gtcgatacct ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag
     4261 cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc
     4321 tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaatgc ggcatcgctt
     4381 tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc
     4441 aatgccgata tcattcgtga agcggctaaa catcacgaag tcggcctgca cgcctgggat
     4501 caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat cgacgatatt
     4561 gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct
     4621 gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac
     4681 aacagcgatt gtcgtggggc catgccgttc cgtccattgc tcgaatcagg aaaccctggc
     4741 actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa
     4801 gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt
     4861 tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc
     4921 aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg
     4981 ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc
     5041 caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt
     5101 ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa
     5161 cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccaac
     5221 tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc
     5281 aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt
     5341 taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc
     5401 tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg
     5461 tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa
     5521 tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg
     5581 gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc
     5641 catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta
     5701 tcagttgcgt actgacggtt ctcccttggg gactggcgat agcgcagcgg gagcctgact
     5761 tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactggat gatgcccaac
     5821 atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg
     5881 gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct
     5941 atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc
     6001 ccacctatat tctttcctgc tttgcatctc tggcaatgct gatggcgcat tacgctttgc
     6061 tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg
     6121 gcgtcactgg cattattgcc acatttgtgg tctccccgtg gggaccaatg aacacgccgg
     6181 tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg
     6241 catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggcctttt gccgcgcttt
     6301 gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtt atggaaggaa
     6361 aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc
     6421 ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca
     6481 tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga
     6541 gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta
     6601 ttactctcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg
     6661 cagatgccat cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg
     6721 gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc
     6781 aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc
     6841 gctggcttgt cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt
     6901 aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa
     6961 actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg
     7021 cattgtgatc ctcgggagta cggtgtaatg ggcctgatgt ggggattatt cagcgtcatt
     7081 attgcctcag ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc gccgatgacg
     7141 cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta
     7201 ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct acataaactt
     7261 gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca
     7321 atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt
     7381 attatgagcg ggttgatgct gattttcctg cccatgacaa aacaacgtta ctgagttttc
     7441 cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact
     7501 ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt
     7561 aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg
     7621 ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg
     7681 accagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta
     7741 acagataaac tctttatcaa cggcaatgga atggcagaaa tgagaagtga aacggagggt
     7801 taattattgc tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc
     7861 gtttttcagt tcaggcggca acgttaacca acgcaccggt tgctgaaaac tggccagctt
     7921 atctttcacc cattcactaa gatcaacgct ctcgtggtca tactccatca ccgccaccgg
     7981 tcgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg
     8041 tgcagcaatt acgcgctcga cttcttccgg ctgaataccc tcaccgccac tgaagaacag
     8101 attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt
     8161 agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc
     8221 tgccatactg gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg
     8281 cagcggcgaa ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa
     8341 ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa
     8401 ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt
     8461 aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat
     8521 ttgctccaat ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca
     8581 cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc
     8641 gccaaacgga atcaacgata acaccccttc ggcactggca agatgggcct gataagtatg
     8701 gacagcggct ttcggcaacc cggtagaacc cgaggtcaac gtcattgagc acagacgcgt
     8761 cggctgccac gtagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaacgc
     8821 cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa
     8881 ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg
     8941 cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc
     9001 gctgccctca acaacgccct gcgccgcaaa gcctgaggct aattcatcga cgcgagcaca
     9061 aagctcgcgc cagttaagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac
     9121 ttgccgccag tgacgccacg gccagtcaga gaagatcata acaaccgctc cagtgcatca
     9181 acttccacga caggcagcgt gctacccggc cagcgacgta cctgctgcgc ctgcatcaga
     9241 tccagcgtgt ccagccctgg aatggtgtcc ggcgttaacc aggcggcaat ccgcgccagt
     9301 tgcgttaagc ctaagctcga ttcaatggaa gaactgatca ccgccgtcag ccccagcgcg
     9361 tgcgccgcct gtacctgctc gcgtactttc tccagactgc ccgtgagcgt gggtttgata
     9421 actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caagctttca
     9481 tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg
     9541 cacggctctt cgagaaacgc gatgcggtgg cgataatccg ggttaacata tttggcaaac
     9601 tgctggcctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga
     9661 attgcctcca gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta caatcccact
     9721 ttgaccttcg ccactttctc gcctggcata tcagcaagtt tgaggatcag atcgtcagga
     9781 tcgccattac acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt atctgccagc
     9841 tccgccaatg cacagcttac gccaaaggca acggaaggca tctgcggtag ctcgcaatcg
     9901 cctgccagcc agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc
     9961 tggctgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg
    10021 caaacgtaca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc
    10081 atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc
    10141 tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca
    10201 tgtagaacag catggtggcg ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt
    10261 cggcgttcag cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc
    10321 ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc
    10381 ccatatccag cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tctcgcgctt
    10441 ttttctgccc gacgatacga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga
    10501 ctttcgggcc agtctgaccg aagatggcat tatctgctgc gatagtcagg tcgcacatca
    10561 tgtgcagaac gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg
    10621 gacaggtacg gatctgacgc tggaagtcca gcacgttcaa gtgatgtacg ccggaatcat
    10681 ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat
    10741 cgcctgcgcc agtcagaatg atcacgccga tattgtcgtc ataacgcgca tctgccagtg
    10801 cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa
    10861 tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg
    10921 agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt
    10981 gtcctttagt cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc
    11041 ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc
    11101 cagggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc
    11161 gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc
    11221 aagggttgcg ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt
    11281 gagcgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt
    11341 gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt
    11401 ttgcagcccc ggatgcccgc cttcgacaat aaccccacaa agccccgcca gcccctggca
    11461 agccgccatc atcgccaccc gtccgccaag cgagtacccc actagccaaa agtcaaggat
    11521 gttgtaacta accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct
    11581 aatcgccgcc gaaccaccat gacctgggag atcaacatac aaccgtgagt agtcggcaaa
    11641 cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag
    11701 ccagggtaaa cctggttttc cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc
    11761 tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc
    11821 accgtggtgg ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt
    11881 tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc
    11941 ggcatcagat agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc
    12001 ccgccgttgt tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc
    12061 agcgcgttga gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg
    12121 cttgcccgct gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg
    12181 ttgctgtaca ccgggtaacc tgctggaagt tgcgaaagcg catcaatcag acgtaccacc
    12241 aggctgttac caacaaacaa ttgcccctgt tcaggcagat agtcgcagat gcgatgcgcc
    12301 agttgcgctt cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc
    12361 aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag
    12421 tcggcaatat tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca
    12481 atgtcatcaa caatccagta ctcttctggt tcacagcttg cctgccattg caggagccgt
    12541 ttgcccgtca ggctgcttcc cagttgcacc acaatttgcg cctgctgcag ctcgctggtc
    12601 gctttggcat tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc
    12661 acatcgccaa tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct
    12721 tccgcactca tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg
    12781 cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac
    12841 cagtcaccca gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt
    12901 tcagcaaacg ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg
    12961 atggtagaaa ccagccaacg tgcggggata tcccgggtcg ggcgcggcaa tgaaatactg
    13021 tgcgtggggt gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca
    13081 attagctccg gcgggcgatc ggcggttaac aggatcagtt tttctccggt taacccggct
    13141 tcaatcagtg caggatagag atttgctacc gccgtgccgg aggtcacaat caccgccacc
    13201 ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg ctcatcgaaa
    13261 tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc
    13321 gagccagggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc
    13381 gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg
    13441 gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca
    13501 ttactattcc atttgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca
    13561 ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatatagcc gcacgacatt
    13621 gccgctgatt tttgctgaac gcagggaaac gcagaattcg ctttgttgta atgagagata
    13681 gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg
    13741 cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat
    13801 cacatcatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg
    13861 cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg
    13921 gcagatatct tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca
    13981 ctctcctaac tgctgcgcct gcttatcatc aggattattt gctactgttc ccgccagcgc
    14041 ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa
    14101 agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact
    14161 ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc
    14221 gagcaccact ttgtcgagct ccccttcggc gatggttttc gttgccagtt cgattaattg
    14281 cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg
    14341 caagggcttg atactcacca gtgtggcgat aaattctctt gcctgaatcg catcgtgctg
    14401 aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca
    14461 ttccaggcgt ggtaaaagta aattgccctg cgacgggtca aacgcattca gcccccaaat
    14521 gcgtaagtcg gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt
    14581 aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta
    14641 gaattgcggg tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag
    14701 agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg
    14761 gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa
    14821 gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta
    14881 acggcgtgcc agcaacagtc ctagtaccag cccgacggcc gcgcccacac caattccttg
    14941 ccagggtttt tcgtggacgt agtcatcagc acgataaaca gcctgcttcg cccgatagta
    15001 ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg
    15061 cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc
    15121 cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga
    15181 cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta
    15241 cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc
    15301 cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag
    15361 gtaaacaggc ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac tcatcagttg
    15421 ctgccccact ttttcgccac gcaacgcttc actgacaatc acccgaccta taacgaccgg
    15481 ctcaagatca tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca
    15541 accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca
    15601 attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac
    15661 cgataattca gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta
    15721 tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt
    15781 aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct
    15841 gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca
    15901 gctactgcat accgccttta accctggaaa actggacaag atttttatca gtcaccttca
    15961 tggcgatcat ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat
    16021 ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg
    16081 gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat
    16141 tctcgatgat ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta
    16201 tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc
    16261 tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct
    16321 ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc
    16381 gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt
    16441 cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg
    16501 cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag tcggtaagct
    16561 aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga
    16621 atgcaggtca attttcccgg cgactgaact agcgaatgat ttcaccgtgt ttaacgttta
    16681 actctgctta ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat
    16741 atatccaccc acacgggcat attgagcgat ttttgatatt tattcatctc cttacgcact
    16801 atattcagag caataagttg cctcttttta tcttcattca gcacaacctt tgcaactaaa
    16861 aggagcccat gatggttaca gttgtcagca attattgtca attatctcaa acccaactca
    16921 gtcagacatt tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa
    16981 cagcgttatc cggagatgaa gaaagtattg agttactgca taatattgcg ttaggttatg
    17041 atgaatttgg aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg
    17101 agaccctatc gattatccga cttattaaaa atgcctgttt aaaattatat aatttggcac
    17161 ataccgcaac caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt
    17221 tcaaaaagct attcccccct tcgaaattaa tgacaattat cggtgatgaa attcctctta
    17281 tatctgaaaa acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg
    17341 atgatacaaa cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc
    17401 ttcagaagat cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg
    17461 tcccctactc caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc
    17521 catcatggcc gaatgaagtc cagttattcc ccataaatac tggcggacac tggatattag
    17581 tttcgctaca gaaaatagta aataaaaaaa ataataaact acaaataaaa tgcgtcatat
    17641 tcaactcatt gcgtgcacta ggccatgata aagaaaattc acttacgcgt gtcattaata
    17701 gttttaattc tgaactcatg ggagaaatgt cgaataataa tataaaagtt catttaaatg
    17761 aaccagagat aatattttta catgccgatc ttcagcaata cttaagccaa agttgcggtg
    17821 cgtttgtgtg catggcagcc caggaagtga ttgaacaaag ggaaagcaat tctgacagcg
    17881 ccccctatac gttattaaaa aactatgctg acagatttaa aaaatattca gcagaagagc
    17941 agtacgaaat tgattttcaa catcgactgg taaacagaaa ttgttattta gataaatatg
    18001 gcgatgcacg aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa
    18061 atagagcatc cggcaaaaga gtgagttaac atcattgtta attaattcga ccagtcagca
    18121 aatatgctga ctggtcgaac atagtttgct aaacagtcat cactgactga tgtcagtcac
    18181 accatccgcc agttcaatct tcgggtattt ccagcgtatc tcggcacgcg attgctggtc
    18241 tgttgatgta gtggattgtt gttgctgact tttcttaccc tcagagttct gccccctgtc
    18301 aactcaatct gccaataaat aacataatta tatttttcct tatatgaatt cctttttaaa
    18361 atagcgaagc cactgtaaaa gatacttttt gtatagatct aacaaccgta ataaaacaaa
    18421 cactcat
//
LOCUS       DAZGCS010000095        18181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGCS010000095 DAZGCS010000000
VERSION     DAZGCS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.095"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(300..1892)
                     /gene="lsrK"
                     /locus_tag="ACHK01_003628"
     CDS             complement(300..1892)
                     /gene="lsrK"
                     /locus_tag="ACHK01_003628"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO8098525.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1971..2924)
                     /gene="lsrR"
                     /locus_tag="ACHK01_003629"
     CDS             complement(1971..2924)
                     /gene="lsrR"
                     /locus_tag="ACHK01_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO8098526.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3173..4708
                     /gene="lsrA"
                     /locus_tag="ACHK01_003630"
     CDS             3173..4708
                     /gene="lsrA"
                     /locus_tag="ACHK01_003630"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO8098527.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4702..5730
                     /gene="lsrC"
                     /locus_tag="ACHK01_003631"
     CDS             4702..5730
                     /gene="lsrC"
                     /locus_tag="ACHK01_003631"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO8098528.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5730..6722
                     /gene="lsrD"
                     /locus_tag="ACHK01_003632"
     CDS             5730..6722
                     /gene="lsrD"
                     /locus_tag="ACHK01_003632"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO8098529.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6734..7756
                     /gene="lsrB"
                     /locus_tag="ACHK01_003633"
     CDS             6734..7756
                     /gene="lsrB"
                     /locus_tag="ACHK01_003633"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO8098530.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7783..8658
                     /gene="lsrF"
                     /locus_tag="ACHK01_003634"
     CDS             7783..8658
                     /gene="lsrF"
                     /locus_tag="ACHK01_003634"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO8098531.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8682..8972
                     /gene="lsrG"
                     /locus_tag="ACHK01_003635"
     CDS             8682..8972
                     /gene="lsrG"
                     /locus_tag="ACHK01_003635"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO8098532.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9029..9787
                     /gene="tam"
                     /locus_tag="ACHK01_003636"
     CDS             9029..9787
                     /gene="tam"
                     /locus_tag="ACHK01_003636"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO8098533.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9791..10705)
                     /locus_tag="ACHK01_003637"
     CDS             complement(9791..10705)
                     /locus_tag="ACHK01_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO8098534.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLHFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10912..12363)
                     /gene="uxaB"
                     /locus_tag="ACHK01_003638"
     CDS             complement(10912..12363)
                     /gene="uxaB"
                     /locus_tag="ACHK01_003638"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO8098535.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12590..14008)
                     /locus_tag="ACHK01_003639"
     CDS             complement(12590..14008)
                     /locus_tag="ACHK01_003639"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8098536.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14147..14506)
                     /locus_tag="ACHK01_003640"
     CDS             complement(14147..14506)
                     /locus_tag="ACHK01_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO8098537.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14506..15432)
                     /gene="glsB"
                     /locus_tag="ACHK01_003641"
     CDS             complement(14506..15432)
                     /gene="glsB"
                     /locus_tag="ACHK01_003641"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO8098538.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15496..16884)
                     /gene="sad"
                     /locus_tag="ACHK01_003642"
     CDS             complement(15496..16884)
                     /gene="sad"
                     /locus_tag="ACHK01_003642"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO8098539.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16985..17866
                     /locus_tag="ACHK01_003643"
     CDS             16985..17866
                     /locus_tag="ACHK01_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8098540.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17943..>18181
                     /gene="yneK"
                     /locus_tag="ACHK01_003644"
     CDS             17943..>18181
                     /gene="yneK"
                     /locus_tag="ACHK01_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO8098541.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTL"
BASE COUNT         4585 a         4640 c         4514 g         4442 t
ORIGIN      
        1 ctttcaatag gaaaattatc atcatcatat ttaacaaaga atagcactaa ttgctaaaaa
       61 tcgaagttta ttaaacccct tttgcatcca ttaatgtctt taggaatatt cgctataaaa
      121 taagggttat gcttaatgct ttaagaaaaa tagcaatttt cccttgaata tagtactggt
      181 gatggaacga tgaatctgca agctggcatt taacaagcca gctctaaaag aagggaaata
      241 agaaaaacta tactcaaaaa ctaacagcca cggtcatcat gatgtggctg tcaatgaaac
      301 tataacccag gcgctttcca taacgacgtc gtcagtccat gatcaaccag ccccagctga
      361 tcctgataaa ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg cttttccggg
      421 tctggtgtgt gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc cattgatgaa
      481 aaaattccgg caccgacgcc agctgcaatg gcacatccta atgcggtggc ttctttgact
      541 accggaatat tgacgggtaa tcccgagaca tcagcgagaa tttgactcca taatttccct
      601 tttgaacctc cgccagcaaa aaccagcgag gtgggatgaa tattcgagaa atcagcaatt
      661 tgctgcaagt tacacgctga tacaatcgcc gcattttctt ccagcgcacg gaacaatgtc
      721 gctttgttac atttatccgg gtcaatggac aagttaataa aggaaggcgc agcgtgatac
      781 caggttttaa agcgcattct gtcggagaag atcggcatta cgccccacga cccaggcggc
      841 acccgactgg ccatctcttc cagcagcgta taggtgtcga tgcctaaacg ttcggcaatc
      901 agtttttctt cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc ggtaaaaaag
      961 cttatagatt cagcttgtac catgccagga ataacatgag gattaacgcg cacgttcatt
     1021 tctgggtctg tcaccggcgc ggctaaattt acaacttgct gccagaatgt gccgccaaga
     1081 accgcggttt gtgccggacg cacaacgcct aacccaaggc aaccaagctg cacgtcgcct
     1141 cctccaacga ccaccggagt gcccgccttc agaccgcaga gttccgccgc ttgtgaactt
     1201 accacgccca gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc acgcaggcca
     1261 gccatatcca gcaatgcagg tttccagtca cgggtggtta gatcaagaag tcccgtggtg
     1321 ccagcgttag agggatccac cgccagttcg ccgctgagca tataggccag ccagtcgctg
     1381 atcatggtga tggttgatgc ctgacggtaa atatcggaac gatggtgcgc cagccaaagt
     1441 aatctgggga tggcacttaa agccagtgtt tgtccggtcg cgcgataaac ttcgttttca
     1501 aaggtattgt tgtgcagttc tttaagttcg ctaacttcgc gtgccgctct ggcatccaca
     1561 ttggcgcagg cccagatcgg ggctccttca ttattatata aaacaatgcc ttcacgcatc
     1621 gaacatgccg aaacggcagc gatatactcc ggggctatgc cggcgttgtg cagcgcctgg
     1681 cgcatacact cacacgccag ttgccagttt ttgttgagat caaattccat agaaccagga
     1741 acgtccggta ctgccaggtg ccgccactcc gcctgtccca ctgctatttg attgccttcc
     1801 aggtcgaata tcacagcccg aatacttccg gtacctgcat ccagcgccat caggtaatac
     1861 tttgattctg aaggggtaaa gagtcgagcc attagattat cctcggctta tgcgttctgt
     1921 ctgttcctct atacgttctc catcattccc ggtaataagg tcatgcaaat ttaactacgt
     1981 aaaatcgccg ctgctgtgtc ctgatcggta accagtgcgt tgatataacc gcctttcatt
     2041 gcagcggcaa ttgcttcggc tttattttct ccccctgcca cgccaacccg gacgggtatg
     2101 gtcttcagcg agcttaaagg taagccaatc agttcgttat gtattttaat atccgtgaca
     2161 acgtcacctt ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc ttttcgacca
     2221 atcattaact gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc gtcgtcctgt
     2281 tgactgacgg ccccaatgcc gacaatcgcc acatcggctg cttgcgcggc taacagaaca
     2341 tctttgacgc aattttcatt ttttagcgta cgggcaatgt cagcggagga tgcccgcaac
     2401 ggagccggaa taatattcac actgcacgcc gcgttaagct gcccgatacc cgtcatataa
     2461 gaaccgacgc caccggagag cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt
     2521 aagcgttgca gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt
     2581 tgaagtaaac tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc gacatcagca
     2641 tccgcaagcc cagggatcac ccggacatgt tgcagcgaaa actgacgacg taattgagtt
     2701 tcatattcca gacagccttc aaagcgagaa ttaatctgta cgcgaataat gccggactga
     2761 tgccctttct ccagcaagcg cgacactttc aaacgggtca ggccgagacg atcgctgatc
     2821 tcgctctggg tcagcccgtc gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct
     2881 tcacacattc cctgttctga aattgccgaa tcgttgattg tcataattca ttcttcactt
     2941 tgaacatatt taaatcttta atgcaattgt tcagttcttg cgcatttata tctgtgatgg
     3001 cagccacagt ttgactctac gagcatgaac aaacgcaacc gtgaaaatca aaatagcata
     3061 aattgtgatc tattcgtcgg aaatatgtgc aatgtccacc taaggttatg aacaaattaa
     3121 aagcagaaat acatttgttc aaaactcacc tgcaaaactg aacgggggaa atatgcaaac
     3181 gagtgatacc cgcgcgttac cgctactttg cgcccgctcg gtttataaac agtattcagg
     3241 ggtcaatgtc ctgaaaggca tcgattttac gttgcatcag ggggaggtcc acgccctgct
     3301 cggcggcaat ggtgccggta aatcgacgtt aatgaagatt attgccggta ttacccctgc
     3361 tgatagcggt acgctggaga ttgggggcaa caactacgcc agattaacgc cagttcatgc
     3421 tcatcagctg ggcatttatc tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat
     3481 aaaagaaaac atcctgtttg ggatggcaaa aaaacagctc tccatgcaaa aaatgaagaa
     3541 cttgctggcg gcgctgggct gccagtttga tctgcatagt ctggcaggat cgctggatgt
     3601 cgccgatcgc caaatggtgg aaatcctccg cgggctaatg cgcgactcgc ggattctgat
     3661 cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt ttactcgctt
     3721 gcaagagctg cttgctactg gcgtgggtat tgtttttatc tcgcataagc tgccggaaat
     3781 tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga accatcgcct taagcggcaa
     3841 aaccagcgaa ctgtctaccg acgacattat tcaggccatc accccagcgg tacgggaaaa
     3901 atcgctctct gccagccaaa aattatggct ggagttacct ggtaaccgcc cacaacatgc
     3961 tgccggaacg ccggtgctga cactggaaaa cctgaccggc gaaggtttca ggaatgtcag
     4021 cctgacgctc aatgccggag aaattctggg cctggctggg ctggtggggg ccggacgcac
     4081 agaactggcc gagacgctct atggtctgcg tactttgcgt ggcggacgca ttatgctgaa
     4141 tggtaaagag atcaataaat tatccactgg agaacgttta ctgcgcggtc tggtttatct
     4201 gccggaagat cgccagtcat ccggactgaa tctcgatgct tcgctggcct ggaacgtctg
     4261 cgcccttact cataaccttc gtggattctg ggcgaaaacc gcgaaagata atgccaccct
     4321 ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag ctgcacggac
     4381 attatccggt ggcaaccagc aaaaaatcct cattgccaaa tgcctggaag cttcgccgca
     4441 agtattgatt gtcgatgagc cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta
     4501 ccagctgttg cgcagcatcg ccgcacaaaa tgtggcagtg ctgcttatct cctccgatct
     4561 ggaagagatc gaactgatgg cagatcgcgt gtatgtgatg catcagggcg aaattgccca
     4621 ctctgcactg accgggcgcg atattaatgt cgagactatt atgcgcgttg ccttcggcga
     4681 tagtcagcgt caggaggtgt catgctgaag tttattcaga acaaccgtga aatcacggca
     4741 ctgctggcgg tggtgctgct gtttgcatta cccggttttc tcgaccgcca gtatttaagt
     4801 gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc
     4861 gcgacgctgg taatgcttac gcgcaatatt gatgtttcag tgggttcgat taccggaatg
     4921 tgcgcagtgc tgttggggat gttactgaac gcggggtatt cactacctgt tgcttgtgtc
     4981 gcgactttac tgcttggttt gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta
     5041 aaaatccctg ccattgttgc cacccttggc acgttagggt tgtacagagg catcatgttg
     5101 ctgtggactg gcggtaaatg gattgaaggg ttacccgccg aactgaaaca gctctccgca
     5161 ccgctgctgt ttggcgtttc cgcgattggt tggttgacga taattctggt ggcatttatg
     5221 gcctggctgc tggcaaagac ggcgtttgga cgcagttttt atgccacggg cgataattta
     5281 cagggcgctc gtcaactggg tgttcgtacg gaagccattc gcattgtggc attttcgttg
     5341 aacggctgca tggcggcact ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc
     5401 aaccagaccg gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt
     5461 agtttgctcg gcggttccgg tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg
     5521 cagatcgata gcgtactggt gctgttgcgc attccggcat ggtggaacga ttttatcgcg
     5581 ggcctcgtgc tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt
     5641 aatctacggc ggcaaaaata tgcccgcttt atgacgccac cgccatccgt taaacccgct
     5701 tcgtcaggta aaaaacggga ggccgcataa tgcgtattcg ctacggttgg gaactggctc
     5761 ttgccgcact gctcgttatt gagattgtcg catttggtgc aattaacccg cgaatgttag
     5821 atctcaatat gttgctgttc agcaccagtg actttatctg cattggcatt gtcgccctac
     5881 cgctgacgat ggtgattgtc agtggcggga tcgatatttc gtttggttcg accatcggcc
     5941 tctgcgccat tgcattgggc gtactgtttc aaagtggtgt gccgatgccg ctggcgattc
     6001 tcctgacctt actgctcggc gcattgtgcg ggctgatcaa cgccggatta attatctata
     6061 ccaaagttaa cccgctggtg attacgcttg gcacgctgta tctgtttgcc ggaagcgccc
     6121 tactgctttc cgggatggcc ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg
     6181 cgtttacaga tttcgctaac ctggatgtgc tgggactccc cgttccgctg attatcttcc
     6241 tgatatgtct cctcgttttc tggctctggc tgcataaaac ccatgccgga cgtaatgtgt
     6301 ttttgattgg gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt aaccgcacct
     6361 tatgtgcgct ctatgccatg acggggctgg cgtctgcggt cgccgctgtg ctgctggtgt
     6421 cgtattttgg ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg
     6481 ccgtggtgct tggcggtgcc aatatttatg gtggttccgg ttccattatc ggcaccgcca
     6541 ttgcggtttt attagtggga tatttgcaac aaggtttgca aatggcagga gtgccaaatc
     6601 aggtgtccag cgccctttcc ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta
     6661 gcctgcatcg ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac ccattgccat
     6721 aaaggatatc ttcatgacac ttcatcgctt taagaaaatc gccttactta gcgctcttgg
     6781 cattgccgca atctctatga atgtgcaggc cgcagagcgt attgcattta ttcccaaact
     6841 ggttggcgtg ggatttttta ccagcggtgg caacggcgca caacaagcgg gtaaagagct
     6901 gagcgttgat gtgacctacg acgggccgac agaacccagt gtttctggtc aggtacagtt
     6961 gattaataac ttcgtcaatc aaggttataa cgccattatc gtttctgcgg tttcgcctga
     7021 tggcttgtgt ccggcactga aacgcgccat gcaacgtggt gtgagagtgc tgacctggga
     7081 ctctgatact aaaccggagt gccgctctta ctacattaat cagggaacgc ccgcccagtt
     7141 gggaggtatg ttggtggata tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc
     7201 gtttttctac tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc
     7261 gaaaatcgcc aaagagcatc ctggctggga aattgtcact acgcagtttg gctataacga
     7321 tgccactaaa tcattacaaa ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc
     7381 cattatcgcc cccgacgcca acgccctgcc cgctgccgca caagccgcag aaaacctgaa
     7441 aaatgacaaa gttgcgattg tcggattcag tacgccaaac gtgatgcgtc catatgtgga
     7501 acgcggcacg gtgaaagaat ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt
     7561 gtatgtcgcg gatgcattat tgaaaaaagg atcaatgaaa acgggcgaca agctggatat
     7621 ccagggcgta ggtcaggtgg aagtctcgcc aaacagcgtt cagggctatg actacgaagc
     7681 ggatggtaat ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg
     7741 caaatacgat ttctgatgtg cattacttaa ccggagtaag ttatggcaga tttagacgat
     7801 attaaagatg gtaaagattt tcgtaccgat caaccgcaac aaaatatccc ttttatcctg
     7861 aaaggttgcg gtgcgctgga ttggggaatg cagtcacgct tatcgcggat atttaatccg
     7921 aaaacgggta aaaccgtgat gctggccttt gaccatggtt attttcaggg gccgactacc
     7981 ggacttgaac gcattgatat aaatatcgcc ccgctgtttg aacatgccga tgtattaatg
     8041 tgtacgcgcg gcattttgcg cagcgtagtt cccccagcga ccaataagcc ggtggtactg
     8101 cgggcgtcag gtgcgaactc tattctggcg gaattaagta atgaagccgt ggcgttatcg
     8161 atggatgatg ccgtgcgcct gaacagttgc gcggtggcgg cgcaggttta tatcggcagt
     8221 gaatatgaac atcagtcgat caaaaatatt attcagctgg ttgatgccgg aatgaaagtg
     8281 ggaatgccga ccatggccgt gactggcgtg ggcaaagata tggtgcgcga tcagcgttat
     8341 ttctcgctcg cgactcgaat cgccgctgaa atgggggcgc aaattatcaa aacctattat
     8401 gtcgaaaaag gttttgaacg gattgttgcc ggatgtccgg tacccattgt tattgctggc
     8461 ggtaaaaaat taccggagcg cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc
     8521 gcttctggtg tggatatggg gcgtaatatt ttccagtctg accatccggt ggcgatgatg
     8581 aaagccgtac aggcggtggt tcaccataac gaaacggctg atcgggcata tgaactctat
     8641 ctgagtgaaa aacagtaact gcggatttaa ggagaagaat tatgcacgtc acactggttg
     8701 aaattaacgt tcatgaagac aagattgacg agtttatcga agtttttcgc cagaaccacc
     8761 tgggctctgt acaggaagaa ggcaatttgc gcttcgatgt cttacaggac ccagaagtga
     8821 attcgcgctt ttatatctac gaagcctata aagatgaaga cgcagtggca ttccataaaa
     8881 ccacgcccca ctacaaaacc tgtgtcgcga aactggaatc tttaatgacc gggccgcgta
     8941 aaaaacgtct gttcaatggg ttgatgccgt gaggcgaatt catcagtttt atctacaatt
     9001 ggggtaacgc gctgacggga gtaaataaat gtctgactgg aacccctctt tatatctgca
     9061 ctttgccgct gaacgatcgc gtccggcggt ggagctgctt gccagagtac cgctggagaa
     9121 tatcgaatac gttgccgatc ttggctgtgg cccaggtaac agcaccgccc ttctaaacca
     9181 acgttggcct gctgccagga taacaggcat cgactcgtct ccggcgatga ttgctgaagc
     9241 gcgcagtgct ttgccagact gccagttcgt ggaagcagac atccgcaact ggcaaccgga
     9301 acaggcactc gatctgattt ttgctaatgc ctcactgcaa tggctgcccg accactacga
     9361 attgtttcct catctggttt ctttacttaa tccgcagggc gtgctggcag tacagatgcc
     9421 agataactgg ctggagccga cccatgtgct catgcgcgaa gttgcctggg aacaaaacta
     9481 cccggatcgc gggcgtgagc cgttggctgg cgttcatgct tactacgata ttttgagcga
     9541 agccggatgt gaggtcgata tctggcgaac cacctactat caccagatgc cgtcacacca
     9601 ggcgattatc gattgggtga ctgccactgg attacgtccg tggttacagg atctgaccga
     9661 gagcgaacag cagctttttc ttaagcgcta ccatcagatg ctggaagagc agtatccact
     9721 gcaagagaac ggacagatac tgctggcatt tccgcgtctt tttattgttg cccggcgtat
     9781 ggagtaaata tcacgtcagc tggtaatgac gatcgggaag aatttttgct ggaatttcgg
     9841 cttcatcgtt catctgtaac aggtcaattt caatagcgtt gcagatggca tccagtggta
     9901 aatcattgtt ttcagtaccg aacggatctt ccagttcttc cgccagacaa tccagcgaaa
     9961 taaaagtgta ggaaatcagc acagagataa aaggcgtcat gtaatgcagg tccacgacca
    10021 gcgcgaacgg cagcatgata caaaacagat aaacggtacg atgcaaaatc agggtgtagg
    10081 caaagggaat tggcgtatag gcaatgcgct cgcatcctgc caggaccgct gaaatatcat
    10141 taagccgatc gttgaggcta ataaacagga tatctgaaag ctgcccattg cggcgctgaa
    10201 ccgccaacca ctcccccatt attaacaaga tacggttagc tggagagttc gaagccagta
    10261 caggctgaag atcttcagtc ttgagataat gagccagcac ttccgcctgt ggctgtttgc
    10321 gtaatgtcat gcgtaaacag tgggcgaagg cgatttgcaa ccgggcaaac tcccttacac
    10381 ttgccgaatc cggcaatgtc gtttttacct cgcgcagtaa cgaccgtgag gcaatcatca
    10441 actgccccca aagttttcgc gcttcaacgt aacgggcgta cccggcatta ttacgaaaac
    10501 caagaaaaat ggcgatggcg acaccgagaa tgctgaacgg tgcgagggtg aatttgatgc
    10561 ccagatgcgt gtaccagggc agcatgaaaa taacagcgat agaaaagaga aaatggagta
    10621 gtaagcgcga tgatatcttg gataatactg agccgtgcca gacaaaaata cggcgcagcc
    10681 agtgttgttg tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc gccctattaa
    10741 acgtgattac gatcacattc tcaagacgct atttacaaat tacctacaag cttacaacat
    10801 taacaggcgc tgcatgtggc atctgttaat gataaaggca cataggatgt tgtaactaat
    10861 atagtgaaat aagaaacccg gtcgaaaccg ggttcagaag taacggtgtt attagcacaa
    10921 cggacgtaca gcttcgcgca tccctttttc aagaatcgca tccaggtcat tagcaacctg
    10981 ctcgaccaga ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac
    11041 aatcgctacc agttcctggg taccgataac acggtcacga tgctggctcc agagttgctg
    11101 ataacgttcc agccagtgtg catcgtcctg taccggatat gtttctccgt tacgctcacc
    11161 acgatagaac gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccgtt
    11221 tgccttctgc cctgccagca gctgcggcag gatgcgggtg cggaacttgg tcataccgtt
    11281 aagcgcgata gacagcagct gatgcttaat gtacgggtta cggaaacgcc cggtgactgc
    11341 actggcgaaa gattccagtt catcacgagg caaatccagt accggaataa tttcttcgta
    11401 gatagctttt tcaacgaatg cgcaaatttc agcatcgttc atcgcttcac ctacggtatc
    11461 cagccctgcc tggaacgcca ccggcaccag cgcggtgtgc gcaccgttga ggattgccac
    11521 tttgcgttct ttgtacggtt taatatcgtc aacaatcagc acgttgagcg gatatttgtc
    11581 cagacgcagt tcagtcgcta aggatttcgg tccctgaatc acaaacaggt aaaagtgttc
    11641 agcggtgtcg agaaaaccat cgtgataacc cagctcttct tccagtttag ccacttcatc
    11701 gcgcggataa ccggtaacga tacggtccac cagcgtagaa cagaagctgt tagcctgatc
    11761 cagccattga ataaatgctt ctggcagtgc ccactcttgc gcatagcgca gcaccagttc
    11821 acgcaacgcg tcgccattgt agtcgatcaa ctcacacggg atgatgatcc aacctttatc
    11881 cagcgcaccg ttgaaatggc taaaacgttc gaacagcaga cgggtcagtt ttgccggata
    11941 acttaccgct ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac ccgcttcggt
    12001 ggtattagag aaaacaaagc gcatttccgg gttgtgagcc agtttcagga attcatcgta
    12061 ttcactgtag acgctgattt cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc
    12121 ttcccccttc tcattcaggc cacggataat ggtggtatac agaccatcct gcgtgctcag
    12181 tgacggtggg aatgaagttt caatcggacg aacaacgacc acaccagaat tcagatcggt
    12241 gtgctcattc aggagatcga tttgccagtc aacaaaggcg cgcaggaagt taccttcacc
    12301 aaactgaatg atacgttctg gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt
    12361 cacaatgggt tcccttctga ttagtcatac aacctgtttg aattggtacg acaggttagc
    12421 aaactttaat acgccgaacc cctgttttga tcaactcctg atgattaatg agcagtttta
    12481 tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa tacgcctttt cctggatcat
    12541 aaagtggtag aacacattgc attcaaatcg cgcggaatga ataaagatgt cagacaactt
    12601 cctcaccgta acgcatagtg ctggtacggt tgcgcccatc tttcttcgag cgatacagac
    12661 acgtatcggc ttcgaccatc aatttattaa aatcatcggt cagcgttcgg tacgatgcgc
    12721 gaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc caggtgaatg
    12781 gttgcagttc aacgccttta cggatttttt ccgccattag cagaccatcg acaggattca
    12841 ccgacggcac tgcaacagca aattcttcgc cgcccatgcg cgccaccagc cccttatcac
    12901 cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca cattcatgtc
    12961 catagttatc gttaatgctt ttgaaatagt caatatcaag cagcatgacc gtcagatgtt
    13021 gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt gagtaaacct
    13081 gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt cattagcgaa ttgatcgctg
    13141 ccacgctaaa agaaaccata attgggcata tcgccatcgt ggcaataccg agacgtgcgg
    13201 agaacatttc cggaatggag aacggcgaac cgaccgagat atcaatcacc gaatttgcca
    13261 ccagtacgat ttccaccgca ccggtgacaa aggtcaacag acatgttacc tgcggcgtat
    13321 agcgcactgc acaccagatt aatgcaggca gcgggaacgc cagactcccc gctccgccaa
    13381 tgactaccga ggcaataacc gacacaataa gcgcgatggc gggcatcatc tgttctgctt
    13441 taaagcgcgg cagcactcca ggaatagcca acgtcagcat acaaggcacg atcaacacgc
    13501 ccgttgagaa ttgctcactg aaccagtccg caagcaaagg ccagaaatcc agactatcaa
    13561 tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaatc agacagtaat
    13621 taaatagccg taaggcactt actggctcat acttattttt ccccagacgc ttatcccgca
    13681 cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata accagtgaaa
    13741 ccagtcccca ttcggtggtt atggcatcat aaaccagcat cgccacataa ctgatcgcat
    13801 agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt acgccattca
    13861 gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata aaatagaaca
    13921 gcgtggttag cgcaaaaata gcgatgctat tacgtagcag atgaccttcc tggaacaaac
    13981 ggaaagttga aatggggtgt acatgcatta attatctgtc catacaattg cgggtggcaa
    14041 ttatctcaac gtaataagta aattcacata aaggatagca tgttcgctaa cagcggcggc
    14101 aaataaaggt accctgggaa tgctatttta tcgttttccg caattctcat ggctgattca
    14161 tctgtacaac cagccagtga tagattaccg ccacgatata acgttgttgt tcttcgctta
    14221 acgaaacacc ctgcggaatg ttatgccact tagccagaca accgcgacag caggtcgcgg
    14281 tggcgtgctg agcgataaac accggatgcc cgcgcatggg ggtttgctta ccgtcattag
    14341 ccggtaatgc cggagcaaga cgtttagcga caaaatccgc cgcatgttgt tcgatgactg
    14401 gcgcgccttt ctccaggcaa tactgacgct ctttcatgcc cagacgaaag cgagagcgaa
    14461 attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg
    14521 ccctaactgt ttcgtcaatt gttcaaggac ggcgataccc gcaagcgagt tacctgcatc
    14581 atccagttcc ggactccaga ctgcgatggc catttcatgc ggaacaatcg ccacaatacc
    14641 gccaccaacg ccagatttcg ccggtagtcc cacccgccag gcaaactccc ccgcgttctg
    14701 gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcaccac
    14761 aggttcatca atatgaatag ctttcccctg attagccaga aagacaaacg tccgggccag
    14821 ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac
    14881 gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga
    14941 atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca caccgcttaa
    15001 gccgcgcacg acttccagca tacgttgccg tggtgcgctt agtcgccctt gcaacatatc
    15061 gcagaccacc agcgcaccgg cattaatgaa cggattacgc ggaataccct gttccatttc
    15121 cagttgcact aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat
    15181 ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg
    15241 aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat
    15301 agcaatcccc aatcgggaac cgtctactgt agccagcgcc ggaatataat ccgcgacttt
    15361 accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat
    15421 ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga
    15481 gctgaatatg tcgcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg
    15541 taagccgaaa tgggaaagct cacgaccaaa gccactcttt ttcacgccac caaaggccac
    15601 tcgcgcgtcg ctggcacaat aaccattgat aaacacccca ccgcattcca gacgtgccgc
    15661 catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact
    15721 atcattagcc agttccagcg catgttctgc atctttcgca acggtgattg ccgcaacagg
    15781 gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca gaaccgttgg
    15841 cggatagtag ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg
    15901 cgccagggtt ttctccacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg
    15961 tccgagagcg ttctcttcat cacggggatc gcccattttc aaggctgccg cagctgccac
    16021 aaaacgttcg gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc
    16081 acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag
    16141 atcggcatcg ttaagcacaa taaatggatc cgaaccgccc agttccagta cgcatttttt
    16201 cagtgccgct ccagcctgtg cgccaatagc cgctcccgca cgaacacttc cggtcaccgt
    16261 gacagcagca atgcgcgaat ctttaatcat ttgactgaca ccgtcgttgt cggcattcag
    16321 ccagccatat acgccttgcg ggattcccgc atctttaaac acctgggcaa tgagctgagc
    16381 acagcccatc acattcggcg catgtttaag taagtagccg ttacctgcaa gaatgatggg
    16441 aaccgcgcca cgcatcacct gccataacgg aaagttccac ggcataatcg ccagaatcgt
    16501 ccccaacggt cgatactcaa taactgcctg ctgattttcc accagcgtag gttccgcctt
    16561 cagcattgcc ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc
    16621 agcgcgcgcc tgattgattg gtttgcccat ttcacgggtg atcatttgcg ccatttcttc
    16681 gctacgggcg cgcagagcct taccgatacc acgcagtttt tcagcacgat aatctatatt
    16741 tgtctcgcgc cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt cgatatcgtt
    16801 agcgccagcc cacggcagca cagaaagttg ttcacccgtg gcaggattta ttgaaattgc
    16861 atgagttgcc ggagtaatgg tcatcggggt atctccttta tgagtcatgg tatgaagata
    16921 cgcagattta ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa
    16981 tattatggat ctgacacaac tggagatgtt caacgcggtt gccgaagctg gcagcataac
    17041 ccaggcagca gcaatagtgc atcgcgtgcc gtcgaatctc actacccgtc tacgccagct
    17101 ggaaacagaa ctgggggtag atctgtttat tcgcgagaat cagcgtttac gtctctctcc
    17161 tgccgggcat aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg
    17221 gagcgttgtc gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac
    17281 cgcagcagtg cgcattccgg ccacgctggc ggaattcaac catcgttatc ccaaaattca
    17341 gttttcactt tccaccggcc cttccggcac tatgctggaa ggagtgctgg agggaaaact
    17401 gaatgcggca tttattgatg ggcccattaa ccatactgcc attgacggga taccggtata
    17461 ccgcgaggag ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc gcgccagtca
    17521 ggttaacggc agtaacattt atgccttccg cgccaattgt tcgtatcgtc gccacttcga
    17581 gagctggttt catgctgacg gtgccgctcc gggaactatt cacgagatgg agtcgtatca
    17641 cgggatgttg gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctctatgct
    17701 ggaaagtatg ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg
    17761 gttaacaacc tggctggtct ggcgtcgcgg tgcgaaaacg cgtccgcttg aggcatttat
    17821 tcaactactg gatgtgtctg actcggcaaa acagagttac caatgagcta ttttgatagt
    17881 tcttgcggct aatatgctct atatagtgat gttccgatga cttatgacta tatggggcaa
    17941 atatggttac gccagtaagc atcagcaatt acatatctct tcctgatgat tttcccgttc
    18001 gtaatattgc accacaggta aaagaagttt taaaggactt tattgatgcg ctaagtacaa
    18061 taatatgtga tgaagagtgg cgcacgtctt taaacatcaa ctcagccaca aaaaagatat
    18121 ttaataacct tgataactta tcatatattc agaggacttc ttttcggggt aacgacacgc
    18181 t
//
LOCUS       DAZGCS010000096        17946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGCS010000096 DAZGCS010000000
VERSION     DAZGCS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.096"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..133)
                     /locus_tag="ACHK01_003645"
     CDS             complement(<1..133)
                     /locus_tag="ACHK01_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO8098542.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     "
     gene            complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHK01_003646"
     CDS             complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHK01_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO8098543.1"
                     /translation="MAKVNMYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFLKNDDE"
     gene            1024..1626
                     /locus_tag="ACHK01_003647"
     CDS             1024..1626
                     /locus_tag="ACHK01_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8098544.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTEHQNQ
                     RRLAYASDVYRIYTGIKSSKGIQIPATKKSLPEINSVQNN"
     gene            complement(1747..3084)
                     /locus_tag="ACHK01_003648"
     CDS             complement(1747..3084)
                     /locus_tag="ACHK01_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO8098545.1"
                     /translation="MPLLNDLLDFSDHPLMPPPSAQLFAGHLPVEWIQHCLTLSAHAT
                     VRRRRLPGDMVIWMVVAMAFFRNEPITDVVRRLNLSADGEAGMNLLARSAVTQARQRV
                     GAAPVEWLFRQTAQTWGSERYLKDDWHGLQLFAIDGAPFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILLNAVTAPYRRSETVPAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTVQTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSLLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRREATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLK"
     gene            complement(3363..4180)
                     /locus_tag="ACHK01_003649"
                     /pseudo
     CDS             complement(3363..4180)
                     /locus_tag="ACHK01_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(4299..4585)
                     /locus_tag="ACHK01_003650"
                     /pseudo
     CDS             complement(4299..4585)
                     /locus_tag="ACHK01_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4610..4816)
                     /locus_tag="ACHK01_003651"
     CDS             complement(4610..4816)
                     /locus_tag="ACHK01_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8098546.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQIPAKRQNRGSRNSKDDGG"
     gene            complement(5362..5553)
                     /locus_tag="ACHK01_003652"
     CDS             complement(5362..5553)
                     /locus_tag="ACHK01_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098547.1"
                     /translation="MNINTETREILRNYRAVINVRRRDMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(5550..5972)
                     /locus_tag="ACHK01_003653"
     CDS             complement(5550..5972)
                     /locus_tag="ACHK01_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8098548.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(6019..6420)
                     /locus_tag="ACHK01_003654"
     CDS             complement(6019..6420)
                     /locus_tag="ACHK01_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8098549.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLGFLYAEASVYVLAKQMMP
                     EYEGGFWHFIRLPDGDRFHLVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSGDA
                     GLTHLYRMRDAQSWSHIEVHPECNAIYAALD"
     gene            complement(6837..7613)
                     /locus_tag="ACHK01_003655"
     CDS             complement(6837..7613)
                     /locus_tag="ACHK01_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098550.1"
                     /translation="MNENTTLNALICRHARDLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTLRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCLEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(7659..8145)
                     /locus_tag="ACHK01_003656"
                     /pseudo
     CDS             complement(7659..8145)
                     /locus_tag="ACHK01_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(8105..8326)
                     /locus_tag="ACHK01_003657"
     CDS             complement(8105..8326)
                     /locus_tag="ACHK01_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098551.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(8327..9010)
                     /locus_tag="ACHK01_003658"
     CDS             complement(8327..9010)
                     /locus_tag="ACHK01_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO8098552.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(9087..9392)
                     /locus_tag="ACHK01_003659"
     CDS             complement(9087..9392)
                     /locus_tag="ACHK01_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098553.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSSVGLTRYRTR"
     gene            complement(9396..9813)
                     /locus_tag="ACHK01_003660"
                     /pseudo
     CDS             complement(9396..9813)
                     /locus_tag="ACHK01_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9736..10856
                     /locus_tag="ACHK01_003661"
                     /pseudo
     CDS             9736..10856
                     /locus_tag="ACHK01_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(10997..11959)
                     /locus_tag="ACHK01_003662"
     CDS             complement(10997..11959)
                     /locus_tag="ACHK01_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO8098554.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDVSVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPIINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(11956..13161)
                     /locus_tag="ACHK01_003663"
     CDS             complement(11956..13161)
                     /locus_tag="ACHK01_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8098555.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSYSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            13532..13705
                     /locus_tag="ACHK01_003664"
     CDS             13532..13705
                     /locus_tag="ACHK01_003664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098556.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCAELDNDQ
                     LNEIIEWVRLYQR"
     gene            13879..14130
                     /locus_tag="ACHK01_003665"
     CDS             13879..14130
                     /locus_tag="ACHK01_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO8098557.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            14127..14414
                     /locus_tag="ACHK01_003666"
     CDS             14127..14414
                     /locus_tag="ACHK01_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8098558.1"
                     /translation="MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            14416..14821
                     /locus_tag="ACHK01_003667"
                     /pseudo
     CDS             14416..14821
                     /locus_tag="ACHK01_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15467..16444
                     /locus_tag="ACHK01_003668"
     CDS             15467..16444
                     /locus_tag="ACHK01_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO8098559.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSNQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(16728..17468)
                     /locus_tag="ACHK01_003669"
     CDS             complement(16728..17468)
                     /locus_tag="ACHK01_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8098560.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(17592..17711)
                     /locus_tag="ACHK01_003670"
                     /pseudo
     CDS             complement(17592..17711)
                     /locus_tag="ACHK01_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            17783..17905
                     /locus_tag="ACHK01_003671"
                     /pseudo
     CDS             17783..17905
                     /locus_tag="ACHK01_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4517 a         4633 c         4410 g         4386 t
ORIGIN      
        1 tgcaaaaaat aaattttcca gtttcgtccc tgatacaggc tggatttgga taatcctgaa
       61 taactgccgt cagattttcc agcagatcta tttgacgagc atgtttttgt tgaatacgat
      121 ccatcggata cataggaacc tcctcacaaa ggattctatg gacagtcgat gcagggagtt
      181 cacgtctccc tgcatcggcg attttcaatt tcaaatttta acgtggcatt aaccacgtaa
      241 ttaatatact aattatcttt cgtactgtta cctttttaaa tcataaaaac tctgatattt
      301 gaacgaagtc aaatttcttt tattcatcat cattttttag aaaaaatcgt tccatctcag
      361 atgatacctt ctccctgata tcttcaacca tattggcata ttcaaacttt ggatttccgg
      421 agacatgagg actgagagac tcaataccca aaattttcgc tactgatgat tgtgttttta
      481 caacgcattc aagaagcaat ttattaaact cagtctgatt aaatgcagac tctttacgct
      541 ccatctgagc ctcatgtaca cgaagcccca gttcaagaag cattgaagct gttgctgaaa
      601 aactgacatc tttttccctt gccccttcct gtcgacgctt ctcaataatc gcatttattt
      661 tttcataggc atcattgctg atatacatgt tcaccttagc cattaaagat tacctttttt
      721 gaacataaaa taaagttaat ttagaaacac taaaattcat aatgcaaaca gggacgcacc
      781 gctagcagtg cccctggcgg tatcctataa agaaacacac cgcgccgcta gcagcacccc
      841 taatataaaa taatgttttt tataaaaata gtcagtacta cccctacaaa gcggtgtcgg
      901 tgcgttgttg tagccgcgcc gacaccgctt ttttaaatat cataaagaga gtaagagaaa
      961 ctaatttttc ataaaactct atttataaat aaaaatcagc aaaaacttgt ttttgcgtgg
     1021 ggtgtggtgc ttttggtggt gagaaccacc aatctgttga gcctttttgt ggagtgggtt
     1081 aaattattta cggataaagt caccagaggt ggaaaaatga aaaaatggat gttagccatc
     1141 tgcctgatgt ttataaatga gatctgccat gccactgatt gctttgatct tgcaggccgg
     1201 gattacaaaa tagatcctga tttactgaga gcaatatcat ggaaagaatc ccgttaccgg
     1261 gttaatgcca tcggtattaa tccggtaacg ggatatggca gcggactgat gcaggtagat
     1321 tcccagcatt ttaacgaact ggcccgctat ggaattaagc cggaacatct gacaacagat
     1381 ccctgcatga acatttatac cggtgcttat tatctggcaa tagcctttaa aaaatggggc
     1441 gtctcctggg aggccgttgg tgcatacaat gccggattca ggaagaccga acaccagaac
     1501 cagagacgtc ttgcctacgc atcagatgtt taccggattt ataccgggat aaaaagcagt
     1561 aaaggtatcc agattccggc cacgaagaaa tcacttccgg aaattaacag cgtgcagaac
     1621 aattaatctt acagacattg tcagataccg gttatgccgc aaaagcggca tggaaggcca
     1681 cacacaaaaa tatcatatga gtgataatac tgactggccg aagccccgcg aagcgggcaa
     1741 tgccgttcac ttaagcggag cggtgctgcg tttcaccgga taacgggttt ttgatatctt
     1801 aactgccctc ggccttgatg gtcgggggcg ttttgttatg aacacaactt ccagagcacc
     1861 ccgcagatgc tccagtcgtt tcgggatggt ccccggtgat gccgtatttc ccagcactat
     1921 catttctgtc gcgataaact ggaacgcgaa cttaaagctg atttctgacg ccgctttttt
     1981 gtgtttctca gccgcttttg ttgcctcacg ccgtatcagg ttgtaagcca gcaacatgcc
     2041 ccacacctcc tgttccagca gctcaacctt acggcttctc agcaccaggg cattgttcag
     2101 caggctgctt ttcaggtttc tgaacccgac ttcgatttcc catcgtgaat ggtaaagttc
     2161 cgctacctct tttgctttgt agcggtctgc cgggagtgat gtctgcaccg ttttctccac
     2221 accgttcacc tcataagtca ccgcccgggc gtaccagaac tctggcagtg caggattctt
     2281 tttcctcgcc tgcagcgaca ccttcagttt cagaagcctg tctccgggac cgtaactttc
     2341 ttctgtttct gccgcgatat tcttccacgc aggcagtagc cagtggcggt tacaaccctg
     2401 ctggttcagc gtcagcagca ggtctgcgct gtaaaagagc ttgtcaaaca gcgtaatgga
     2461 gttatccggg atagtggcga gcatggagtg agccggcacg gtttcgctcc gccggtaagg
     2521 cgcggtcacg gcattcagca aaatgtggct tcccagattc atcaacgcca ccagacgcat
     2581 gaccgggtag gcgctctgcc gctcagtgga tgtgttggca gagccataat attcacgcaa
     2641 ctcaggttca tcaggtgtcc tgaacggtgc gccatcaatg gcaaaaagct gcaggccgtg
     2701 ccagtcatcc ttcaggtaac gttccgagcc ccatgtctgt gcggtctggc ggaagagcca
     2761 ttccaccggg gcggccccca cgcgctgacg cgcctgggtg acagcgctgc gggccagcag
     2821 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaaccacat cggtaattgg
     2881 ctcattgcgg aaaaaggcca tggccaccac catccagata accatgtccc ccggtaaacg
     2941 acggcggcga acggtcgcat gcgcagaaag cgtcagacag tgctgtatcc actcgacggg
     3001 aaggtgccct gcaaacagtt gtgcagaggg cggtggcata agcggatggt cactgaaatc
     3061 gagcagatca ttgagaagtg gcataagaaa acggctccct gttgtggaag ccgttatagt
     3121 gccgcagttt aaggatcggt caaccgatcc ttaactgatc ggcattgcgc gaagcgggaa
     3181 cccatcttat atataaccgg taactgcgca gcctcctgcc agaccatttt tcgcgggcac
     3241 aatatttaca gacaaccgga aagccagttg atgaaattga ggtatatcag acgacacagc
     3301 gatgccgaag aatgataata taagtaaagc cccgaaaaat tttcggggct ttacttatat
     3361 tatcatgact tccactttct gaactgttcg gcaatcaccc ataacgcctt gttgattctt
     3421 atatcaccat caatacctgt aatggctctt gtcctggtat ttttcccgga ggcacttcgc
     3481 cccgataatc caccttttat catattctcc tgagcccgct gccaggttgt ccagagatcg
     3541 ttctgtttat tctcccgacg acggggagta attatctgtt caggcgtcac tggcgtttta
     3601 ttttcatctt catacctgac catcagtgca gctctgccaa ataaatgttg ctcgtcactg
     3661 ttaagatgaa tttctttcat cgcctccata ttatcagtga ccttatcaaa gacaccgagt
     3721 acttcatacg ctccctcgat aacctgcccg acaatatcac ctttatgtgg aacgcggatt
     3781 tcgccaaaat tatttccgca caccaggcca tttgtgcaga caaaacgaaa aattccgggg
     3841 atcatctgat aactggatga accatcatgt gaattaagca ggataatttc aggaacttcc
     3901 tgcccgttaa tatgcccttc cctgcgaaga cgtaacatat gtttactgta ttcgcgacgt
     3961 cccaaatcac gaacccgact ctgacaggca aagaatggct ggaaaccttc atcacgtaac
     4021 ttattgatga tattgttgga atatacgtat aacgttcact ccgggactca tgtttatcac
     4081 cggaaaatac cgaaggcacg aactgcatta attcatcatc cgttaaagga cgttcacggc
     4141 ggatggaatt ataccgacca aaacgggaag ctaatcgcat aacagctact cctttatgaa
     4201 agaataaatt taataaaaaa cttttccgct taatatcatc cctcccggtc actattaaca
     4261 agattgcaac aatcaggaga aatattcatc acatctggtc agcagatcgc ccggatatcg
     4321 aggccggcac gcttaatctg acgcaggaac tgtttgaagt gatcggcttc cgcaatgttg
     4381 atatggcgga actgcgtatc cacaccccgg ctgagcgcgt agctatagct gaaggccatc
     4441 ttttccgggt ggcttttgcg atattttttc agctcagtca ggctgctgaa ttccaggacg
     4501 tcccactctt ccccggtgtt tttttcttcc cggcaacaag aagcgtgtac gacgcggtgt
     4561 taacgtggat atttcgggta ctcatcctgc tttctcctct ctctggtttt cagcccccat
     4621 catcttttga gtttcgggag ccgcggtttt gacgttttgc ggggatctgc cttttgttcc
     4681 ctccggtatt caccatcacc tttttgagaa ccttcgccgc aaggggcatc actgccacgg
     4741 ccggaaacga ctgtccgggg gcagaccgca aaagtttttg cctgcttttg ggcaaaaagt
     4801 tttgcgggat gtacatttca ccctggactg gcggagacgt ctttgccccc ggcgacaggg
     4861 gcttaaacag gtgaatggca ccggacacaa caacgggcag ggacccgccg caacgacgga
     4921 gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc ggccttttgc cggaatgcca
     4981 ggagcgcagg ccacggcgcg acagaggcgg tgcgaccgga acggtcgctg caagccaggg
     5041 tttcgcgtca gggatggaag cccgccaggg cggagacagc ttcgctggct ccgttcacga
     5101 cagcccgccc cgccaggggg acgcccggaa aagccataaa caggagagga acggaaagaa
     5161 gaggaagagg aaaaagaaga agaacaataa caagcacggt aaccatagaa aaataacatc
     5221 agaaaaaaac attccggaca catcacaaac aggaagggta acgggcgacg cgtcagcatg
     5281 gcccgcttcc cttccggcag gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg
     5341 gcctccaccg cctgccctgt atcacctgtt tctgctgccc tgaaggatat agtgaccacc
     5401 gaggaaaacc gcctgctgat tcgccacaaa atcgcagatt tcatcaacaa tctgcgcggt
     5461 ggtgagcggt ttctgcccca tatcacgacg ccgcacatta atcacggccc tgtaattacg
     5521 cagaatttcg cgggtttccg tgttgatatt catgacatgt gctccccgtc cagcgcctgt
     5581 aacacaacgt ccattgcttc gcgttcttcc ctgacgaatg cgtcaccgtc gcccccgttc
     5641 tcactcccga cggcatacag gcgcttcatt tcactgcctg ccagcgccat cactttttca
     5701 agcatcactg ccgggactgt cacctgacgg ctggcacgct tttcttccat cagttcacgg
     5761 acaacgtcgt gaacaatgct gacatccgca ccatgctcaa aagcatcgtc cagccgttgc
     5821 atgacggttt ccagctcatc gtctgtcaga ctccagtccc gggctatgtg acgcacatcc
     5881 ccacgggtca gcaccacggc aaaaacgcat tcactgtccg gcacgtctgt atccagcact
     5941 tcgcggataa tttctttaac agtacagtac atgttctcgt ctcctttaca ggggcggcat
     6001 tgccgccccg tccgttaatc agtccagtgc cgcgtaaatc gcgttacatt cgggatgaac
     6061 ttcgatgtgg ctccacgact gcgcatcacg catccggtac aggtgtgtca gtcccgcatc
     6121 accgctgtcg tggtacagcc acaactggcg gttaatcaca agggaagtca gaatgatgcc
     6181 tgcggcatcg gcgctgaccg tgcggtcaaa ccagtttgca ccgttaacca gatgaaaacg
     6241 gtcaccgtcc ggcaggcgga tgaagtgcca gaagccgcct tcatattccg gcatcatttg
     6301 tttcgccagc acgtacacag acgcttcggc atacagaaaa cccaggccaa acaggtaagg
     6361 cagaaaggag agacggtcgc actcttcaac attcagagtg acaggtttcg catattgcat
     6421 gatgttttcc tccgttgttc acatattccg cgacaccggg cggtgccgtg cctccataaa
     6481 agccgggcga taaggcgggg atgcaggggc gcaagggaag ccgcagcctg acgcacacgg
     6541 ctggcgggag ttggagggtg agaccgcgaa acctgagcgg gtgaacgacc ggaaaatcac
     6601 ggcagacggg aaggagggac cgggtgaccg cagcggcgaa gcgtaccctt gcatccccgc
     6661 cagcccggct aagatggagg cacggcacag cccgcacaca acacggcggc cacataactg
     6721 aaaacccaca aggccatgcc ccgcatggcc gggtaaagcg gagccggtgg cgacttcctt
     6781 tgagctctgt ccggggaccg gcaggaaaaa ggcggggatt taccccgcca acgtcatcat
     6841 ttccggattt ttgccagggc atccgccatg ctgcggattt cggcccgggc ttcccggaca
     6901 acttcccgca gaacaccacg gtcaaaccac tggcggggcg gttcatccgg tatacgcagc
     6961 acatcgcagg ccagcccact ggacaggata tgttcaagac aatcgctgac cagatacatt
     7021 tccaccgccg aaaaacgtgc gccccggttc aggatggcat cgagcaccac gggcaggttt
     7081 tcgtcgtcct catcgagcca gcaggggtga tcgctttcct tcacgactag gcgaaagcgg
     7141 cgcgtctggc gggtcagtaa cgtctgaccg gtggtggtca gcgccgcccg gatacgcagc
     7201 gtatcttctg ccacccggca actgacactg cgtacagcat cacgcaccgc cgcaagtaca
     7261 gccctgattt cgtcttcggt cagccattca ccggcatacg gaaaagacac ctgcgttccg
     7321 tctgccggaa gtaccggcag cgactgccac tgactgccgg acagtacaag ttccgccccg
     7381 gttccggtca gtttgtgaat agcggaggct aggagcggcg gcagtacgcc gccgtgacgg
     7441 ttgataagta acgtcgccag ccggggcgca tccagcagaa cataaatgct gatccagccc
     7501 ggatacttcg ggttccgctg gtcaacatca gtctcttccg gccagccctg cgccagcagc
     7561 aggtcgcggg cgtgacgaca aatcagtgca ttcagcgttg tgttttcgtt catgattaat
     7621 cgttctccct gtatcgccgc ccggaggcgg cgataacatc agtcgttttc gtgcgtgttg
     7681 tttttcagca ggatgcgggc ctgattaacc accgcctgac ggcgggtgac gaattccggg
     7741 acggcgaggc cgttatcacg ggcggcccat tcacgcttcc acaatgcctg ttcagcggtc
     7801 tgaatcaggg acgccagcag ttcagcaggg acggtcacct ggcggttttc actcacgttg
     7861 ctgataatct ccatagccgc accggaagaa ataccggact cagtccgctg gtcttccggg
     7921 atgtcctcca gccgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt
     7981 ccatcagcga ggacctgaat ctcttccggg gaccagacaa ccagcatcag cggagtgtct
     8041 cccggatact gcgctgccaa ctcccggcag agcgtttcgc atgttccgta catactctct
     8101 cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc tccatcaaga
     8161 ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc cggcagggtg tcggcattaa
     8221 agctgtaccg ctgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     8281 cctccgccac ttcggcgcgc agtttttcgt gtccggcata gttcatttac gcagcctccg
     8341 gttcaaacca gttatcattc gcggccccct gctgcatggc ccgttgcacg gcggccagtc
     8401 gttgctgtcc ggcacggtga tactgttcaa gcagctcgat accgatatac cggcgtccgg
     8461 actgaagggc ggccacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt
     8521 tcggatgtgt gaagctctca atcagtggtt gcaggctggt aacgggcttt tccgtcgggt
     8581 gatggcggtt gcctgaatat ttccagccca gcacgtcggg caatggcttc tgaggcagcg
     8641 ccggacgccc ttttgccagg atgtaagcgc attcgtggcg atagcccaca tatgcggcct
     8701 tcgatgtgta ggttttggta aataccaggt ggccaaccac gctgaatccc gcatttttcc
     8761 aggcggccat aaagcgatcg acgcggttcc agccatagaa gctcaccatc agcgcatctt
     8821 ttttcagcac tcggtacatt tcattacagg ccggttgcag ccattcatcg gttttatcgc
     8881 cggcgatggt acgcccctga cggtcacgga aaccgacgag atatggcggg tcggtgagga
     8941 tgaaatcgac agcattgccc ggaaacgtgg ccataacacg cacgcagtca ccctggataa
     9001 aacgggacat aacagcactc cttctcttac agagggcacc gggagggcgc ccctgtggcg
     9061 cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt aacgggtcag gccgaccgag
     9121 gaataccgca gccgtcgtct ttttgacggc gaggatatgc cgcgaaagcg gcttgaaggg
     9181 cgttcatcag aaaagggcga cgggctggcg tacactgtca ccaacgccag ccgggagcgg
     9241 taaaagcgcc cgtcccgtgc ccggccggac gcgggacact gaccacaggc agcgttcctg
     9301 ccgcgccgcg tcagccggcg cagtaaaaag aggagcccgg cgacatcctt taccggaaaa
     9361 gaaacgggcg gttgcccgcc cgtctccgtc atacttcatc ctccataatg cgccacattg
     9421 tcgactatcc acgccggtcc ggtgacttcc ggcagctcat cgtcatctgt cggggaagac
     9481 cattcaagtt cccccccaca gcacatcaac gagctctcca cgctcataca actgccagcc
     9541 acagaaatca cagccctgtt cggcatacca gtgctccagt gtgcaactga aacggcggct
     9601 taattctgca ataacgtcgc tttctggctg acaccacggc gtgtcaaaat ccacctgaat
     9661 gaagttgtct ctctgactgc tgatattcac ctcatacccc acaggccact tcacgccata
     9721 ctgttcggta taccagtgat atgcagaagg aacaccgttc agaacaccac cgttaaagcc
     9781 gtttacttca acatccaggc ggctgggcat caaaatgggg taccgtggtt tcagctgaag
     9841 tcggtgaaat tttcggagcc ggtcatcgtc ttctccagta attacccgct atacgcatcg
     9901 atgtcgaaca gggtcatggt tcatctggag gcgctggcac cgcgcgttga gcagtactcg
     9961 attgacgaga tgtttctcga cgtgcgaggt attgacagct gcatcgattt cgaggatttc
    10021 gaccagcagc tgcgtgaaca cgtcaggaac gggactggcc tgactatcgg tgtcggaatg
    10081 gggccaacca aaacgttggc aaagtcaaca caatgggctt ccaaagagtg gccacagttt
    10141 ggtggcgtgc tggcattaac gccggacaat atccgccgtg cggaaaaact gctgtcactg
    10201 cagctggcgc acgaaccctg tatttatcag gaagaacttc aatgtggttc tggagagaaa
    10261 ggtccgggaa ctcaatggag aaagctgtat ttcactggag gaagaaccgc ccccccaaac
    10321 agcagatagt ctgcagccgg tcctttagag agcgcgtcac gacatatgag gccatgcgct
    10381 aggccatctg tcggtacgct gagcgtgcag ctgaaaagct gcggggcgag tgtcagttct
    10441 gcagacatat cgccattttt ttgaagacgt caccgttcgc agtaaacgaa ccgtattacg
    10501 ggaatgtggc cagtgaaaaa ctgctcatcc ccacgcagga tacacgggat atcattgccg
    10561 ctgccgttag aattctggac aggatatggg tggatggcca tcgatatgca aaagcgggtt
    10621 gcatactcaa cgatttcact cctaccgggg tttcgcattt gaaccttttc gatgatactc
    10681 agccccggag caacagtaac cagccgttga aagtcgtgga cagcattaac cattccggtc
    10741 tggggaaagt gtggtttgca ggccgaggca ttgcgccaga atggcagata aagcgtgaga
    10801 tgtcgtcacc ggcatacact acccgctgga acgaactacc ggtcgccaga ctttgaaaaa
    10861 aagaaattca tggtgatttc actatgaaag aattaaaaaa aatactttat attcagtaaa
    10921 ataagcatga aaaaagcgtg gtgaaaattg caaaaaaagg atgccatttg gcatcctgat
    10981 attaacccgg catatcctat tcaggcagtt ttcttagaat gttttttata gcctcatcga
    11041 tttctaactg cacctcatta gatatgcggg agaactcata aacaacaaga cgctttctgg
    11101 ggtcagtctt ctttctggca aattggttac gatcagaaaa ctctttcaac ttctcagttt
    11161 ccactgtttt tacaggccgg tctgtaagca attttccttc agacctgaag accgccagga
    11221 ttttgctttt ctcaattgtt ggatagtcag gtaaagccat cagtttttgg cgaactctat
    11281 ccataaactc ttccactgac atttttttgt tggttgcctt ttcagacagt ttcaacaaaa
    11341 attgatagtc cgacagcgaa atatcattga ttatcggaaa tacagagatc atttcatccg
    11401 gtactgatgc cgcctgaaat gcgcgtgtta cttttgccgg agagatgctt tcagcctgag
    11461 cgatctcttc ttttgtcata cttgccccat aagtaacttc aaggcgtttt cctagttctc
    11521 ggaggctatg ctctctggct gtctgtatat cttttgcaag ttgtctcgcg tcggcaatat
    11581 caatactgtc ttttgtaacc agtatttcga attttacgtt gttatagata caggcagcac
    11641 gacgacgcgt accgtctaat acttcaattc gtccatctac ttcccgacca atagccggaa
    11701 aaaattgctg aagtttaatc gtgcgagtta tgtcactaac tgactcacgc gaaagcatag
    11761 tctgatcgcg tccattaaca gatacatcaa cgaacgtttt agattctata tctccgctta
    11821 atacaaccgt tttaatgaat tttgcttgtt tccccgactt aagagtaaac gttttgatct
    11881 ctccgtcacc ttcaagcatg cgagcgaact ctgaattgct attgccgaga actcgccctc
    11941 gagctaaaac ctttttcatt tggatgctcc ccgaatgtat tcaatgcggt caaaaacggc
    12001 tttagtaaat cgttccgctt cggtacgagc ttttttcaat gcctctgcac ttcctggata
    12061 cgaaacaggg ttagcactga tgatagtgtc aaaagattca ccacatcgtt cgaacccgtc
    12121 cagtctcgga agcgatgagt caagaatatt actcgcatac acttcacgag ccaggctata
    12181 ggaagtttcg tgatcgcgct tactggtcat tttggacata aaccctatgc tcgcactcag
    12241 gcggggctca acgccttctt cttccaaacg ttctagcatt tcaggtaaac gagtcaaata
    12301 tttgagagtt gaatggaaat caacttgcgc cgggggagta ggggtgagaa gtaaatcact
    12361 cgcagccagg ccattgagaa gaaatggatc aagatgaggg ccagtatcaa taaaaacaaa
    12421 atcataatca tcagcgatac ggtcaatgat ggttttcctg agtacttcag aaggcttaaa
    12481 accaggaagg tgttcagcaa ccaaagactc ccattggctt gctacgaagc cgtcatcaat
    12541 agaagcaggg atgacatcaa cgccaggaat catagtgggt cggataaccg cttctcgcag
    12601 tgtttcagca tcgagattat ttagcattgc ttgggcggca gtttcaagga ctgtaccaat
    12661 actatgggta tggtccaaaa acatagtgct ggacgcctgc gggtcaaggt cgatcacaag
    12721 gatacgaagg tcatgacgca gcaaatccgg atgtactcgt agtgcatgcg ccagcgttac
    12781 tgtactgacg gtttttgaaa ccccaccttt caagttgacg acaaaaatta cgtaaggttc
    12841 tttatgaaca tcgcgatatt ttggtatctg tcggtgagcg taaatatcaa taacgttctg
    12901 aattgttaat gcatactgct cgacgttgcc tacccgcttt ttattaaatt catagccact
    12961 ttcttccatt tctttaacgg ccagttctac gatgcgtcgg ctcagcttcg gcaattttgc
    13021 gaccgcatta cgagtatatg tttgatgata ctctgtcaga ttgaattctt ttctttgctc
    13081 ctcaatatcc cgactcatgg cctgaagtag agcacttgct ctgagcgcta tggtaccgac
    13141 actaccgtaa tctcgtttca tagttttcct ctgagataga ttccctgccc atatattgcg
    13201 tacaaagttc aatcacattg tacacagata tgaaagaggt acaatatatt ttgcattgcc
    13261 attaaattat cgtacgttgc aagtcaccat tcacatcaaa ccgcatagtt agacattgtc
    13321 tctgagcagg cgaggggtta gacaagaccg ttgggcgcgt cagagtgtta tattaggata
    13381 cgggtaggtg acacccacct ctggcacaga acggacattc attgatgcca tgccagaatg
    13441 gacgttcagg ttattccgtc cagttctgct ggcagcacga gatctcccct ggtatagtga
    13501 tgccacggca aagtgctcaa acagggataa tatgatggaa atcaaggctc aacagtttgt
    13561 cacatcaacg gggcggcaag tccttactga caacggacaa caaggtatgg gcggcgtggc
    13621 ggaagcaatt tttgcaaact gcgcggaact ggataacgac cagcttaacg agatcatcga
    13681 gtgggttcgg ctctatcagc gctgaatgcc atcatcaggc tgcgcaagcg gcctttttta
    13741 cgccccttgt ttaattcccg cactacctgg acattcaggt gattctgtcc atctgtataa
    13801 aaaacaacca aaagacttgt taacaggtca tataaggagt atctttgaga ctggttaaat
    13861 agtcttgaaa ggtggcctat gcctaacatt attctcagtg atacaagcgc cagtgtcagc
    13921 gagctgaaga aaaatccgat ggcgacagtc agcgccggtg atggtttccc ggtcgctatc
    13981 ctgaaccgta atcagcctgc tttttactgt gtacccgcag agctgtacga aaagatgctc
    14041 gatgccctgg acgatcagga gctggttaaa ctggtagccg aacgcagcaa ccaaccgctg
    14101 catgatgtag acctagataa atacctatga ggtatcaggt aaaattcagg gaagatgcgc
    14161 tgaaagagtg gcaaaaactg gacaagacta ttcagcaaca gtttgcgaaa aagctaaaaa
    14221 agtgctgtga caatccgcat attccttccg caaaactgcg cgggataaag gactgctaca
    14281 aaataaaatt acgtgcgtca ggttttcgcc tagtctatca ggtgattgac gaacaattaa
    14341 ttatcgctgt tgtagctgtg ggtaaacgtg agcgcagtga cgtttataat cttgccagtg
    14401 aaagaatgag ataattcacg gcggattatc ggttcagccc tctcattaac cgctgatgct
    14461 gagttgatgt gtagggcttt gcgtaatgca ctggaaacac gaccgcgtga tggtcgcctg
    14521 ctgtttcatt cagatcaagg tgttcagtat aaaagcaata aatacagaaa gttactttat
    14581 ggggtaatgc aaagtatgag tcgcagaggg aattgcctgg ataattcgcc gatggaaaga
    14641 atctttcgca gccttaaaag tgaatggctt ccgaaaggtg gttatggtga ttgccatgca
    14701 gtacgcgaat aaatcagtgg ataaatgctt attacaacgt ttatcgacct catacaaaca
    14761 atggtggttt atcgccttgc cgtatgaaga aaaatggaaa caggttattc cggtgtcctg
    14821 attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca ttgcacatcc
    14881 tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca ggcgacttct
    14941 gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg cgaaccggcg
    15001 aggaagcgca atgtatctgt agttacagtg agcaggtacg ccctgaagcc ctttcataaa
    15061 acggttgtgt gcgctgtcct tcgggaagat ttatccacat atccagaggc ccgatccagt
    15121 aaacagatcc atgaatgatc aataaaggat ccattaaaga tccccatacc gctgcaaacc
    15181 ttgccactca tgctccagaa ccacgatcac ataagctgtg gcatgttgat gataaactgt
    15241 aacatgctga tgataagctg tattcagtaa tacatatact gacgtaagtt aatgacataa
    15301 actatggtca gtacaccaga ctcagttgtt aaatacagac tgcaggtttt tcttcagtca
    15361 gttgacgggg gtctgacaca cgattttctg tttattcttt tactgtccac aggcaggagg
    15421 ctttctggaa aacgaaaatt cagacatcaa aaaactgttc ggcgaggtgg ataagtcctc
    15481 cggtgagctg gtgacactga caccaaacaa taacaacacc gtacaacctg tggcgctgat
    15541 gcgtctgggc gtctttgtac cgacccttaa atcactgaag aacagtaaaa aaaatacact
    15601 gtcacgtact gatgccacgg aagaactgac acgtctttcc ctggcccgtg cagagggatt
    15661 cgataaggtt gagatcaccg gcccccggct ggatatggat aacgatttca agacttgggt
    15721 ggggatcatt cattcctttg cccgccataa cgtgactggt gacaaagttg aactaccttt
    15781 tgtcgagttt gcaaaactgt gtggtatacc ttcaaaccag tcatcacgca ggctgcgtga
    15841 gcgcatcagc ccttccctga aacgcattgc cggtaccgtg atctcctttt cccgcaccga
    15901 tgagaagcac acccgggaat acatcactca tctggtacag tcagcctact acgatactga
    15961 acgggatatt gttcagttac aggctgatcc ccgccttttt gaactgtacc agtttgacag
    16021 aaaagttctt ctccagctta aggcgattaa tgccctgaag cgacgggagt ccgcccaggc
    16081 actctacaca tttatagaga gcctgccccg ggatccggca ccgatatcgc tggcgcggct
    16141 gcgtgcacgc ctcaatctga agtctccggt attttcccag aaccagacgg tcagacgggc
    16201 aatggagcaa ttgcgtgaga ttggatatct tgattacact gagatccagc ggggacggac
    16261 aaaactcttc tgtattcact accggcgtcc ccggttaaaa gcgccgaatg atgagagtaa
    16321 ggaaaatccg ttgccacctt cacctgcgga aaaagtcagt ccggagatgg cggagaagct
    16381 tgccctgctt gagaaactgg gcatcacgct ggatgacctg gaaaaactct tcaaatcccg
    16441 ctgaacataa actgtagtca gtgaagatcg ctcctttact gactacagct tatattaacc
    16501 ggggcagtga gtggtttgct cactgcagtt tatattctgt ttcctgcagt gctgtctgta
    16561 gctgagctgc catctgctgt cccttacgta agtgatcccg taacctgatg ctgaggcatt
    16621 gctcccttca taaaacatga cttactcact acagcttata tacatgctcc agcttatgtt
    16681 atgtctgttc tgctgaccac tgcttatata aggaagcgcg tatgccgtca cctcagggga
    16741 ggcagtgtac gcaggatctc cgcagcatcc cgcccgtcac ctgtgaaagg cactgccagc
    16801 gtggcagcca tatccagtgc aaacactctg gtataaacct ccatagaacg tggatccctg
    16861 tgaccagcca gtgcctgaat gactttccgg ggctggcggt gataaagcat gtgcatgata
    16921 tagctgtgcc ggaaggtgtg tggtgtaacc ggaatcgaaa agtgtactcc gtcagcttcg
    16981 gcccgtctga cagcctgctt cagccagttg cgcatggttt cgtcggtcac ggcccataat
    17041 ggttcacgac gacggggccg ggtggtgatc atccagcttt ccatctgcct gacatagctt
    17101 atatctgtca gcggaactag gcggacttca tcttttggcg ggcgtccgcg tcgcgcacgc
    17161 actttttcgg acaggatccg cacaaacggt cttgcaccat ccagatcaaa tgattccggc
    17221 gtcagcatcc gggcttcgcc aatacgcatt ccggtattcc agagggtggc gaacagcata
    17281 tggtgacgct gatccggcat atagaagaga agggcactca cttccggggc cagcaggtag
    17341 gccggtgtgg cccccgtgga agtggccatt cttctcaggg agagcgctgt tgcaaaatcc
    17401 acccccggcg caatgggtaa cagggagacg cgttctggtg aattctgcag gggaatgaca
    17461 ttgttcatga gttatttttc acgtagcaca cgcactgttt atggatacag gtattctgac
    17521 ctgaacgccc ggatccttcc acgacaataa atcgcagcgc gcggatcctt ttgtttgatt
    17581 atcacgcatt gttgtgggta aaaccaaata cgtaaaggcg cattttgccg caggtatccc
    17641 gtcttaagcc agtcatggtg cgggttgaga gcttgtttac cgtttttggg agaattgggg
    17701 taatgccagg agaggcagac atgggtaaac agagttcaga caggaaagaa gtgcaggtac
    17761 gttacgtaaa tcctaaccaa gttttgatat gcgattcgaa cgcactgcat tatctgctgt
    17821 agaaaatagc ttccggatat tcagtagttc tctgggaata tcccgatgga aaaatatgca
    17881 gcagataaat gaatatggca atagagtgca atttattcat tacgggatgc taatctacac
    17941 ataaaa
//
LOCUS       DAZGCS010000097        17748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGCS010000097 DAZGCS010000000
VERSION     DAZGCS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.097"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..574
                     /locus_tag="ACHK01_003672"
     CDS             <1..574
                     /locus_tag="ACHK01_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFO8098561.1"
                     /translation="IIRAMMEGGTAKIYHCNDSDKCLKVVADTPVTISRDNALKSQIT
                     KLLASIQNKAVSDTPLDDKEKGFISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYD
                     ILLQYIQELIQQARAMVATGNYDEAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVV
                     DRQMSYMRQQLSARMLSRYQNNYHFGGSTL"
     gene            571..3392
                     /gene="traG"
                     /locus_tag="ACHK01_003673"
                     /pseudo
     CDS             571..3392
                     /gene="traG"
                     /locus_tag="ACHK01_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490585.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            3407..3910
                     /locus_tag="ACHK01_003674"
     CDS             3407..3910
                     /locus_tag="ACHK01_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO8098562.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            3942..4673
                     /gene="traT"
                     /locus_tag="ACHK01_003675"
     CDS             3942..4673
                     /gene="traT"
                     /locus_tag="ACHK01_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO8098563.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            4925..7078
                     /gene="traD"
                     /locus_tag="ACHK01_003676"
     CDS             4925..7078
                     /gene="traD"
                     /locus_tag="ACHK01_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8098564.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDLSGEFVKSYYDPSIDKILNPLDAHCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            7078..12348
                     /gene="traI"
                     /locus_tag="ACHK01_003677"
     CDS             7078..12348
                     /gene="traI"
                     /locus_tag="ACHK01_003677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO8098565.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLNELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFADLGREINTQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     VRENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            12368..13114
                     /gene="traX"
                     /locus_tag="ACHK01_003678"
     CDS             12368..13114
                     /gene="traX"
                     /locus_tag="ACHK01_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO8098566.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            13173..14033
                     /locus_tag="ACHK01_003679"
     CDS             13173..14033
                     /locus_tag="ACHK01_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8098567.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            14136..14696
                     /locus_tag="ACHK01_003680"
     CDS             14136..14696
                     /locus_tag="ACHK01_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO8098568.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDETVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            14829..15041
                     /locus_tag="ACHK01_003681"
     CDS             14829..15041
                     /locus_tag="ACHK01_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8098569.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            15286..15747
                     /locus_tag="ACHK01_003682"
     CDS             15286..15747
                     /locus_tag="ACHK01_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO8098570.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            15793..16002
                     /gene="hha"
                     /locus_tag="ACHK01_003683"
     CDS             15793..16002
                     /gene="hha"
                     /locus_tag="ACHK01_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO8098571.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            16040..16192
                     /locus_tag="ACHK01_003684"
                     /pseudo
     CDS             16040..16192
                     /locus_tag="ACHK01_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16193..16599)
                     /locus_tag="ACHK01_003685"
                     /pseudo
     CDS             complement(16193..16599)
                     /locus_tag="ACHK01_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16721..17158)
                     /locus_tag="ACHK01_003686"
     CDS             complement(16721..17158)
                     /locus_tag="ACHK01_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098572.1"
                     /translation="MYFIGYHGTSEASAQNILSVGVQERFLPPTGQIGRGFYIAKMNG
                     TLPQWGAENATEFARRNQPYFTRMVSLLTGASNNPWLDKAAQRTILKIYTVRPLLHCK
                     WNIMNPGDLEFLKATLYDNGGPGLDLKRDCKWLYHQRNSSTCG"
     gene            complement(17407..17634)
                     /locus_tag="ACHK01_003687"
     CDS             complement(17407..17634)
                     /locus_tag="ACHK01_003687"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098573.1"
                     /translation="MSQRQRHSVIIHGDLHMGNILFDEKNNRFWPIDFSNSYEHYYSI
                     DKDSKLFVNKMADREFCSILKTLRENAISNI"
BASE COUNT         4417 a         4096 c         5161 g         4074 t
ORIGIN      
        1 aattatccgg gccatgatgg aaggcggcac agcaaaaatt tatcactgca acgattctga
       61 taaatgcctg aaagtggtgg cagacacacc ggtgaccatc agccgggata atgcactgaa
      121 gtctcagatt actaaacttc tggccagcat tcagaacaag gcggtcagtg acacgcctct
      181 ggatgacaag gaaaaaggct ttatttccag taccaccatc cccgtcttca aatacctggt
      241 tgacccgcag atgctcggtg tttccaacag tatgatttac cagctgacgg actatatcgg
      301 ttacgacatc ctgctgcagt acattcagga gctgatacag caggcccggg cgatggtggc
      361 cacaggaaat tatgacgaag cagttatcga acatattaac gacaacatga atgatgccac
      421 ccggcagatt gcggcgtttc agtcacaggt gcaggtacag caggatgcgc tgctggttgt
      481 cgatcgtcag atgagctaca tgcgtcagca gctttccgcc cgcatgctca gtcgttacca
      541 gaacaactat cacttcggag ggagcacgct gtgaatgaag tttatgtgat tgccggtggt
      601 gagtggttgc ggaataacct gaacgccatt gccgccttta tgggaaccag gacgtgggat
      661 tccattgaaa aaattgcgct cacattgtct gttctcgcgg tggccgtgat gtgggtacag
      721 cggcacaacg tgatggattt gctgggctgg gtggccgtgt ttgtgcttat cagcctgctg
      781 gttaatgtcc gcacatcggt gcagattatt gataacagtg acctggtcaa agttcaccgg
      841 gtggataatg taccggtcgg actggcgatg ccactttcac tgacgacccg tatcgggcat
      901 gcaatggtgg ccagttacga gatgatcttc acgcaaccgg acagtgtcac ctatagcaaa
      961 acggggatgc tgtttggcgc agaactggta tcaaaaagca ccgacttttt gtcccgcaat
     1021 ccggaaatcg ccaatctttt ccaggattat gtgcagaact gcgtgatggg ggatatttac
     1081 ctgaacacaa atacacgctg gaggagctta tggcctccgc tgatccctac acgctgattt
     1141 tttcccgccc cagtccgcta cggggcgttt atgacagcaa taataatttc gtgacctgta
     1201 aggacgcgtc ggtttcgctg aaagacaagc tgaatctgga tacacagagt ggcggaaaaa
     1261 cctggcatta ttatgcccag cagctatttg gtggacgccc tgatccgaac ctgttgttca
     1321 gtacgctgat tggcgacagt tacagttact tctatggctc aagtaaatca gccagccaga
     1381 tcatccgcca gaatgtcacc atcaatgcgc tgaaggaggg gatcaccagt tatgccgcgc
     1441 gtaatggtga ttcagccagc ctggtgaatc tggccaccac gtcatcgatg gagaagcaac
     1501 gtctggcaca tgtctccatc ggtcatgtgg cgatgcggac actgcccatg acgcagacca
     1561 tcctgacggg gatcgccatc ggtattttcc cgcttctggt actggcggct gtcttcaaca
     1621 aactgacgct gtccgtcctg aaaggctatg tgtttgccct gatgtggttg cagtcatggc
     1681 caatgttgta cgccattctg aacagcgcca tgacatttta tgcgaagcag aatggtgcac
     1741 cggtggtgct ctcggaaatt tcacagatac agctgaaata ctccgatctg gcttccactg
     1801 ccgggtatct ttccatgatg atcccgccat tatcctggat gatggtcagg ggactcgggg
     1861 ccggattttc cagtgtgtac agccatttcg cttcctctgc tatcagcccg actgccagtg
     1921 cggcaggtag tgtggttgac ggtaattact cctacggcaa catgcagacg gaaaacgtga
     1981 acggattcag ctggagcacc aacagcacca cgtcgtttgg tcagatgatg taccagaccg
     2041 gcagtggcgc aaccgctaca cagacccgtg acggtaatat ggtgatggat gcgagcgggg
     2101 cgatgtcccg tttaccggtc ggtatcaatg caacgcgtca gattgcggcg gcacaacagg
     2161 aaatggcccg ggaggcgtcg aacagagcag aaagcgccct gcatgggttc agcagcagta
     2221 ttgccagtgc ctggaactcg ctcagtcagt ttggttctaa ccgggggagc agtgattctg
     2281 tcaccggtgg tgctgacagc acgatgagcg cacaggactc catgatggcc agccgtatgc
     2341 gcagcgcagt ggaaagctat gcgaaggcgc ataatatcag taatgagcag gcgacaagag
     2401 aactggcatc aagaagtacg agaggttctg cggggatgta tggcgatgct catgctgaat
     2461 ggggggttaa acctaaaatt cttggtgttg gtggtggcgt aggtgttaga ggtggtggtc
     2521 gggcaagtat tgactggagt gatgaggatg cgcatcaggc cagcagtggt tcacgggcca
     2581 gccatgatgc tcgccatgat atcgatgcca gggctacaca agactttaaa gaggccagcg
     2641 attactttac cagtcgcaag gtcagtgaat ccggcagcca tacggacaat aatgccgatt
     2701 cccgtgtgga ccagctgtct gcagccctga actcagcaaa acagagttac gaccagtaca
     2761 cgacgaatat gacccgcagc catgaatatg ctgaaatggc atcccgtact gaaagcatga
     2821 gtgggcagat gagtgaagac ctgtcgcaac agtttgcaca gtatgtgatg aaacacgcgc
     2881 cgcaggatgc cgaagctatt ctgacaaaca ccagttcacc ggagattgca gaacgccgtc
     2941 gggctatggc gtggtctttt gttcaggaac aggtgcagcc tggtgttgat aatgcctggc
     3001 gtgaatcccg tggggacatc ggtaaaggaa tggagagcgt accttcgggg ggtggcagcc
     3061 aggatattat tgctgatcat caggggcatc aggccattat tgagcaaaga acgcaggaca
     3121 gtaatatccg taatgatgta aaacatcagg ttgataatat ggtcacagaa taccgaggca
     3181 atatcggaga tactcagagc agcatacgtg gcgaagaaaa tattgtagga agacagtatt
     3241 ctgaactaca aaatcaccat ataacatagg cattatctca gaataataaa tataatgaag
     3301 aaaaatcggg tcaggaaaga atgcccgggg ctgacagtcc acaggaactg atgaaaagag
     3361 caaaggagta tcaggataag cacaagcaat aatattgtat ggggagatga taatgaaaaa
     3421 tttagctcat attgctctgg tgacggtgat acagttcatt gcctgttatc tggctgattg
     3481 ggggagtgcc gaaacagttt ttatcctgtg ctttattgta ctatggcagg gattgtttat
     3541 atggttattt tctcaaatac gaaaaaaacg gcatgtgtcg gatgagttta aattcagtaa
     3601 aggcgtttgg tatatactga tgccagtatc ttccttactc tctccgttat taagcctgat
     3661 ggtttttatc attggtacac tatacgaatt aaggcgaatt agtggttgtg taagtgtaag
     3721 agaatggatg caatctcagg taaatgaaca aactaatgaa gatttgcatc tggattttga
     3781 tgatgtgaat tttgattatg tggattttta tcgtactaat ccggcaacag gattagctat
     3841 gcatggagga tttgattcag caggaaatac ttttgggact tgttggcagg actatgatga
     3901 caggcgataa gaaaactgtg gtttgcaata aaggtaattt gatgaaaaca aaaaaattga
     3961 tgatggttgc actggtcagt tccactctgg ccctttcagg gtgtggtgcg atgagcacag
     4021 caatcaagaa gcgtaacctt gaggtgaaga ctcagatgag tgagaccatc tggcttgaac
     4081 ccgccagcga acgcacggta tttctgcaga tcaaaaacac gtctgataaa gacatgagtg
     4141 ggctgcaggg caaaattgct gatgctgtga aagcaaaagg atatcaggtg gtgacttctc
     4201 cggataaagc ctactactgg attcaggcga atgtgctgaa ggccgataag atggatctgc
     4261 gggagtctca gggatggctg aaccgtggtt atgagggcgc agcagttggt gcagcgttag
     4321 gtgccggtat caccggatat aattccagtt ctgccggtgc cacactcggt gtgggccttg
     4381 ctgccggtct ggtgggtatg gctgcagatg cgatggtgga agatgtgaac tataccatga
     4441 tcacggatgt gcagattgca gagcgtacta aggcaacggt gacaacggat aatgttgccg
     4501 ccctgcgtca gggcacatca ggtgcgaaaa ttcagaccag tactgaaaca ggtaaccagc
     4561 ataaatacca gacccgtgtg gtctcaaatg cgaacaaggt taacctgaaa tttgaagagg
     4621 cgaagcctgt tctcgaagac cagctggcca aatcaatcgc aaatattctc tgattatctt
     4681 ccggaggctg gtctgaccgg cctcctgact gacgcctcgc tttgctcgtt gtccaacccc
     4741 ggaaagaaat aaaataaatt ttatttctcc ccggcgtttc cgaaataaat atgaaaacac
     4801 tgagcggttg tgcaaattct ttgtgcgacc gtgaagtgtt gccggcgaag ccggaattta
     4861 acggttatta atcttttcat cattaataat cagtccgact gacttttttc ttcggaatat
     4921 catcatgagt tttaacgcaa aggatatgac ccagggcggt cagattgcgt ccatgcgtat
     4981 ccgcatgttc agccagatcg ccaatatcat gctttactgc ctgtttattt ttttctggat
     5041 actcgttggt ctggttttat gggtaaaaat aagctggcag acgtttgtga acggctgtat
     5101 ttactggtgg tgtaccacgc tggaaggcat gcgggattta atcaagtccc agccggtata
     5161 tgagatccag tattacggca aaaccttccg gatgaacgct gctcaggtac tgcatgataa
     5221 atatatgatc tggtgcggag agcagctatg gtccgcattc gttctggcta cagttgtggc
     5281 actggttatt tgcctgatta ccttctttgt tgtctcctgg attctggggc gtcagggtaa
     5341 acaacagagc gaaaatgaag tcacaggtgg tcgtcagctg acggagaacc cgaaagacgt
     5401 tgcccggatg ctgaaaaaag acggcaagga ctccgatatc cggattggcg acctgccgat
     5461 tatccgggat tccgaaatcc agaacttctg tctgcacggc acggtcgggg ccggtaagtc
     5521 ggaagttatc cgtcgtctgg ccaactacgc ccgtcagcgt ggagatatgg tggtgattta
     5581 tgacctttca ggggaatttg ttaaaagtta ctatgatccc tccatcgata aaatcctgaa
     5641 tccgctggat gcccactgtg ccgcctggga tttatggaag gagtgtctga cacagccgga
     5701 ttttgataat acggcaaata ccctgatccc gatgggcaca aaagaggacc cgttctggca
     5761 gggttcagga cgtaccattt ttgcggaagc ggcgtacctg atgcgtaatg accccaaccg
     5821 cagctacagc aaactggtgg acacactgct ttccatcaaa atcgaaaaac tgcgtacctt
     5881 cctgcgtaat tcaccggcgg ccaacctggt ggaagagaaa attgagaaaa cggcgatttc
     5941 catccgtgct gtgctgacca actacgtgaa agccatccgt tacctgcagg ggattgagca
     6001 taacggtgag tcttttacca tccgtgactg gatgcggggt gtccgggaag atcagaaaaa
     6061 cggctggctg tttatctcgt cgaatgccga cacccatgcc tccctgaagc cggtgatctc
     6121 catgtggctg tccattgcca ttcgtggtct gctggcaatg ggagaaaacc gtaaccgtcg
     6181 tgtgtggttt ttctgtgatg agctacccac gttacacaaa ctgccggacc tggtggagat
     6241 cctgccggaa gcccgtaagt tcggtggctg ttatgtgttt ggtatccagt cctatgccca
     6301 gctggaagat atctacggtg agaaagcggc tgccacgctg tttgacgtca tgaacacccg
     6361 tgcctttttc cgttctccca gccataagat tgcagagttc gctgccggtg agattggtga
     6421 gaaagagcac ctgaaagcca gcgagcagta ctcctacggt gctgatccgg tacgtgacgg
     6481 ggtatcgacc ggtaaggata tggagcgcca gacgctggtc agttattccg acattcagtc
     6541 tctgccggat ttgacctgtt atgtcaccct gcccggaccg tatccggcag taaaactctc
     6601 actgaaatat caggcacgtc cgaaggttgc ccctgagttt attccgcgtg acatcaaccc
     6661 ggaaatggag aaccgcctga gcgccgtact tgccgcaagg gaagcagaag gtcgtcagat
     6721 ggccagcctc ttcgaaccgg atgtgccgga agttgtttcc ggagaagacg tgactcaggc
     6781 tgaacagccg caacagccgg tgtctcctgc catcaacgat aagaagtcag attcaggtgt
     6841 gaatgttccg gcagggggga tcgagcagga gctgaaaatg aaaccggaag aagagatgga
     6901 acagcaactg ccacccggga tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga
     6961 ggcatggcaa caggaaaatc atccggacat ccagcagcag atgcagcgtc gtgaagaggt
     7021 gaacattaat gtgcaccggg agcgcgggga ggatgttgag ccgggagatg atttctgatg
     7081 atgagtatcg cgcaggtcag atcggccgga agtgccggta actattatac cgacaaggat
     7141 aattactatg tgctgggcag catgggagaa cgctgggccg gcaggggggc tgaacagctg
     7201 ggactgcagg gcagtgtcga taaggatgtt tttacccgtc ttctggaggg aaggctgccg
     7261 gacggagcgg atctaagccg catgcaggat ggcagtaaca ggcatcgtcc cggctacgac
     7321 ctgaccttct ccgctcccaa aagtgtctcc atgatggcca tgttaggtgg cgataagcgc
     7381 ctgattgatg cacataacca ggccgtggac tttgctgttc gtcaggtgga ggcgctggcc
     7441 tccacacggg tgatgacgga cggacagtca gaaacggtgc tgaccggtaa tctggtgatg
     7501 gcactgttta accacgacac cagtcgcgat caggaaccac agttacacac gcatgcggtg
     7561 gtggctaatg tcacgcagca taacggcgaa tggaagacac tgagcagtga caaagtgggg
     7621 aaaacggggt tcattgagaa tgtgtacgct aatcagattg cctttggcag gctctaccgg
     7681 gaaaaactga aagagcaggt tgaggcgctg ggctatgaaa ccgaagtggt gggtaagcac
     7741 ggtatgtggg agatgccggg cgtaccggtg gaggcctttt ccggacgctc acaggcaatc
     7801 cgggaggccg tcggggaaga tgcctcgctg aaatcccggg atgtggcggc actggatacg
     7861 cgaaaatcca aacagcacgt cgatcctgaa gtcagaatga ctgaatggat gcagacgctg
     7921 aaggaaaccg ggttcgacat ccgggcatat cgtgacgcgg cggatcagcg tgcggagacc
     7981 cgcacgcagg cgcccggggc tgtttcacag gaggggccgg atgtgcagca ggcggtgaca
     8041 caggccattg ccggattaag tgaacgaaaa gtgcagttta cgtacacgga cgtgctggcc
     8101 agaacggtcg gcatactgcc gccggaaaat ggtgtgattg aacgggcacg cgccggtatc
     8161 gatgaggcca tcagccgtga gcagcttatc cccctcgatc gtgagaaggg gctgttcacg
     8221 tccgggattc atgtgctcaa tgagctgtca gtccgggcac tcagtcgtga catcatgaaa
     8281 cagaaccggg tgaccgtaca tccggagaaa agtgtccccc ggacggctgg ttacagcgat
     8341 gcggtcagtg ttctggcaca ggaccgtccg tcgctggcca ttgtgtccgg gcagggcggt
     8401 gcagccgggc agcgtgagcg ggtggctgaa ctggtcatga tggcccggga gcaggggcgg
     8461 gaggtgcaga ttatcgctgc tgaccgtcgc tcgcagatga acctgaagca ggatgaacgg
     8521 ttgtccggtg agctgataac cggacgtcgt cagctgctgg aaggcatggc cttcacgccg
     8581 ggcagtaccg ttatcgttga ccagggcgaa aaactctccc tgaaagagac gttaaccctg
     8641 ctggacggtg ccgcacgtca taacgtacag gtcctgataa ccgacagcgg gcagcgaacc
     8701 ggtacaggca gtgcgctgat ggccatgaag gatgccgggg tgaacacata tcgctggcag
     8761 gggggagaac agcgaccggc caccatcatc agtgaaccgg accgtaatgt ccgctatgcc
     8821 cggctggccg gagattttgc ggccagcgtg aaagccggag aagagagcgt ggcacaggtc
     8881 agcggagtac gggaacaggc catactgaca caggccattc gcagtgagct gaaaacacag
     8941 ggcgtgctcg gacaccagga ggtgaccatg acggcactgt caccggtctg gctggacagc
     9001 cggagccgtt atctgcggga tatgtaccgt ccggggatgg tgatggagca gtggaacccg
     9061 gagacacgca gtcatgaccg ctatgttatc gaccgggtga cggcgcagag tcacagcctg
     9121 accctgcggg atgcgcaggg tgaaacgcag gtggtgcgta tttcctccct ggacagcagc
     9181 tggtcgctgt tccggccgga aaaaatgccg gtggcagacg gcgagcgact gagggtgaca
     9241 gggaaaattt ccggactccg cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt
     9301 gaagatgcga tgacggttgt tgtaccgggg cgggctgaac cggcctccct gcctgtgagc
     9361 gattcaccat tcacggcact gaagctggag aacggctggg tggaaacgcc cgggcattcc
     9421 gtcagcgaca gtgcgaaggt ttttgcctcc gtcacacaga tggcgatgga caacgcgacc
     9481 ctgaacggtc tggcccgcag tggtcgtgat gtccggctgt attcctcact ggatgaaacc
     9541 cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg tggtttctga gcagataaag
     9601 gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc tgcagaaagc cgggcttcac
     9661 acgccggcac agcaggccat tcatcttgcc cttccggtgg tggaaagtaa aaacctggcc
     9721 ttcagcatgg tggacctgct gacagaggca aagtcgtttg ctgcagaagg aaccagtttt
     9781 gctgacctgg gacgggaaat caatacgcag ataaaacgcg gtgatttact gtatgtggat
     9841 gtggcaaaag gctatggcac aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaag
     9901 agcattcttc gccatattct cgaaggtaag gaggcggtca cgccgctgat ggagagagta
     9961 cctggcgaac tcatggagaa actgacatca ggacagcgtg ccgccacccg catgatactg
    10021 gaaacgtccg accgtttcac ggtggtgcag ggctatgccg gtgtgggtaa gaccacacag
    10081 ttccgggcgg tgatgtcagc cgtgaacctg ctgccggaga gtgagcgtcc ccgtgtcgtt
    10141 gggctgggtc ccacacaccg tgcggtcggg gagatgcgca gcgccggcgt ggatgcgcag
    10201 acactggcgt cctttctgca tgacacgcag ctgcagcagc gcagcggaga aacgccggat
    10261 ttcagcaaca cgctgttcct gctcgatgag agctcaatgg tgggcaatac cgacatggca
    10321 cgggcatacg ccctgattgc ggccggtggc ggtcgtgccg tggccagtgg tgacacggac
    10381 cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc
    10441 gatgtggcta tcatgaaaga gattgtgcgt cagacgccgg aactgcggga ggcggtatac
    10501 agcctgatta accgggatgt ggaaagggca ttgtccgggc ttgagagtgt gaaaccgtct
    10561 caggtgcctc gtcaggaggg cgcatgggca ccggagcact ctgtgacgga gttcagtcac
    10621 agccaggaag cgaaactggc agaagcgcag cagaaggcga tgctgaaagg cgaggctttc
    10681 ccggatgtcc ccatgacact gtatgaagcc attgtccgcg actataccgg caggacaccg
    10741 gaagcacggg agcagacgct gattgtcaca cacctgaatg aggaccggcg cgtactgaac
    10801 agcatgattc atgatgcacg ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg
    10861 cctgtcctga acacagcgaa tatacgtgac ggagaactgc gtcgtctctc cacctgggag
    10921 aataacccgg atgcccttgc cctggtggat agtgtgtatc accggattgc cggtatcagc
    10981 aaggatgacg ggctgataac cctggaggat gcggaaggta acacgcggct gatttcgccc
    11041 cgggaggcgg tggctgaagg cgtcacactg tacaccccgg acaccatccg ggtgggaacc
    11101 ggtgaccgga tgcgcttcac gaagagtgac cgggagcgcg gttatgtggc caacagcgtc
    11161 tggacggtga cagcggtttc cggtgacagt gtcacgctgt cggacggaca gcagacccgg
    11221 gtgattcgcc ccggtcagga gcgggcagag caacatattg acctggccta tgccatcacc
    11281 gcccacggtg cgcagggggc aagtgaaacc tttgccattg cgcttgaagg cacggaaggc
    11341 gggcggaaac agatggccgg ctttgagtcg gcctacgtgg ccctgtcgcg tatgaagcag
    11401 catgtgcagg tgtacaccga taaccgtcag ggctggacgg atgccatcaa caatgccgta
    11461 cagaaaggaa ctgcccacga tgtgctggag ccggggtctg accgggaggt catgaatgca
    11521 gagcggctgt tcagtacggc gcgggagctg cgggacgtgg tggccggccg tgccgttctc
    11581 cgtcaggcag ggctggccgg gggagacagt cctgcacggt ttattgcacc cgggcgcaaa
    11641 tatccgcagc cgtatgtggc actgccggct tttgaccgta acggcaagtc agctggtatc
    11701 tggctgaacc cgctgaccac ggatgacgga aacgggctgc ggggcttcag tggtgaaggc
    11761 cgggtgaaag gcagcgggga tgcgcagttc gtggccctgc agggcagccg taacggagaa
    11821 agcctgctgg ctgataatat gcaggatggt gtccggattg cccgtgataa tcctgacagc
    11881 ggtgtggtgg tgagaattgc cggtgaaggt cgtccgtgga atcccggtgc catcaccggt
    11941 ggtcgcgtgt ggggggatat tccggataac agcgttcagc cgggtgccgg aaacggtgaa
    12001 ccggtcacgg cagaggtact ggcacagcgg caggctgaag aggccatccg ccgtgaaacg
    12061 gaacgccgcg cagatgaaat tgtccgtaaa atggcagaga acaaacctga cctgccggac
    12121 ggcagaacag agcaggctgt cagggagatt gccgggcagg agcgtgaacg ggctgtcact
    12181 tctgaacggg aagccgcgct gccggagagt gtactgcgtg aaccacaacg ggagcgggag
    12241 gcggtccgtg aggttgtccg ggaaaacctg ctgcaggagc gactgcagca gatggagcgg
    12301 gatatggttc gtgacctgca gaaagagaaa accctgggcg gagactgata caggaagata
    12361 aacgctgatg acaacggata acacgaacac gacacgtaac gattcactgg ctgcccggac
    12421 cgatacctgg ttgcagtcgt tgctggtctg gtcacccgga cagcgggata tcatcaaaac
    12481 ggtggcactg gtgctgatgg tactggacca tgcaaaccgc attctgcatc tggaccagac
    12541 gtggatgttt ctggccgggc gtggagcctt tccgttgttt gctctggtgt gggggctgaa
    12601 tctgtcccgc catgcgcata tcaggcagtc ggccattaac cggctgtggg gatggggaat
    12661 tattgcccag ttcgcgtatt atctggcagg ctttccgtgg tatgagggga atatcctgtt
    12721 tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac
    12781 agcagcggcc attctcctga tggcactgtg ggggcctttg tccggcacca gttacggcat
    12841 tgccgggctg ctgatgctgg cagtcagtca ccggctgtac cgggcggaag acagaacgga
    12901 acgtctggtg ctggttgcat gcctgctggc cgtgattccg gcacttaacc ttgcctccag
    12961 tgatgcggcg gctgtagctg gtcttgtgat gacggtgctg accgttggtc tggtgtcgcg
    13021 tgtaggaaaa tcattacccc gtttctggcc cggagatttt ttcccgacgt tctatggctg
    13081 tcatctggct gtgctgggca ttctggcgtt gtgactggta tgatactgac gctgattctg
    13141 cttattaata aacaacataa cacaggatat ttatgaaaat tacggatcac aaactctctg
    13201 aaggtattgc cctgacattt cgtgttcctg aaggaaatat aaaacatcct ttgattatct
    13261 tatgccatgg gttttgcggt atcaggaatg ttctgctccc ttgctttgcg aatgccttca
    13321 cagaagcagg attcgcaacg attacttttg attatcgagg attcggtgag agtgatggag
    13381 aacgagggcg tctggttcct gcaatgcaga ccgaagatat tatttcagtt atcaattggg
    13441 cagaaaagca ggaatgtatt gataatcagc gtattgggtt atggggaaca tcgctgggag
    13501 gggggcatgt cttcagtgca gctgcgcagg accaacgcgt aaaatgtatt gtcagccagc
    13561 tggcttttgc tgacggtgat gttctggtta ccggcgagat gaatgaatcg gagagagcgt
    13621 cttttctatc cacgctgaat aaaatggcag agaagaagaa aaacaccggg aaagagatgt
    13681 ttgtgggggt tacacgggta ctaagtgatg atgaatcgaa ggtattcttc gagaaaatca
    13741 aagcccggca tcccgaaatg gatataaaaa tacctttcct tacggtcatg gaaacgttgc
    13801 aatataaacc ggcagagagt gcagccagcg tccagtgccc agttttggtt gttattgccg
    13861 gccaggatag cgttaatcca ccggaacagg gaagagcatt atatgatgct gttgcttctg
    13921 gtactaagga attatacgag gaagctgatg catgccatta tgatatttat gagggcgcgt
    13981 tttttgaacg tgtagtagct gtgcagacac aatggtttaa acaatatctt taattgtcag
    14041 tatttatacg ttaaaggtcg ttgaaattaa cttcctgtta cgatgagttg tttcaacggc
    14101 ctgcagggca tattttcacg aaggtcagag taagcatggc agagcaaaaa cgaccggtac
    14161 tgacactgaa gcggaaaacg gaaggagaga cgcctgtccg cagccggaaa accatcatca
    14221 atgtcaccac gccaccaaaa tggaaggtga aaaagcagaa gctggccgag aaagcagccc
    14281 gggaagcgga gctggcggca aaaaaagcgc aggccagaca ggcgctgtcc atttatctga
    14341 acctgcccac gctggatgag accgtgaaca ccctgaaacc ctggtggccg ggattatttg
    14401 acggtgacac gccccggctt ctggcctgcg gtatccggga cgtgttactg gaagacgtgg
    14461 cgcagcggaa tatcccgctc tcgcataaaa agctgcgcag ggcgctgaag gccatcaccc
    14521 gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg ccggtatgac acggaagggt
    14581 atgtgacgga gcatatttct caggaggagg aagcgtatgc ggcagcgcgt ctggataaaa
    14641 tccgccgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag aaataaaaaa
    14701 agcctccctc agagagagga ggcagggaaa taaggcttaa aaggaaataa cttccgtacc
    14761 cgaaaggaag ctaattcagt agattattta acaccataat actgacgggt taaagaggag
    14821 tgataaagat gaatggattc agaaacagtt cacggaacgg tcaggtctgg cgttaccagc
    14881 gtgccggaag ccgggccgtt attctggaag tcagtggacg ctggatggaa gcggcagaag
    14941 catggcgacg ggctgccggt gttgcacccc ggacagactg gcagcagttt gcccgaaaaa
    15001 gggctgaaca ctgccaccgg cgctgccgag gaagggggta acgaaaaaag cagccttcat
    15061 tactccaggg attcagaagg ctgaataatg catgagattt gcttttttta atatccggtc
    15121 gggcagtatg accggacagt tctgagtagc acagagtttt ttccgtatta agtcgtgatt
    15181 ttatattttg tgattaattt cacaaaataa ggtgttattc agtgtgtgct gcaatattca
    15241 ggatgcctga aatacaggtg ctatttatct gatacggaga ataacatgag gaaatatatt
    15301 ccactggtat tatttatctt ttcatggccg gtattgtgtg cagatattca tggacgggtt
    15361 gttcgtgtac tggacggtga taccatcgag gtcatggact cacggaaggc ggtgcggatc
    15421 aggctggtaa atattgatgc gccggaaaag aaacaggatt atggccggtg gtcgacagac
    15481 atgatgaaat cactggttgc aggaaaaacg gttaccgtca catattttca gcgagatcgt
    15541 tatggtcgta ttctcgggca ggtttatgca ccagacggaa tgaatgttaa tcagtttatg
    15601 gtccgggccg gggccgcctg ggtatatgag cagtacaaca cagatcccgt gctgcctgtc
    15661 cttcagaacg aagccagaca acaaaaacgt gggctctggt cagatgctga tccggttccc
    15721 ccctggatat ggagacaccg taaataaagc gatggcaatc ttatcattca tctgttctca
    15781 gcgggaataa aaatggaaaa aacaaaacag gaatggctgt atcaattacg gcgatgttca
    15841 tcagtcaata cgctggaaaa aattattcat aaaaacagag acagtttatc gaccagtgaa
    15901 cgtgaatcat ttaattcagc agcagaccac cggttagcag aattaatcac cggaaagtta
    15961 tatgacagga taccaaaaga aatatggaaa tatgtaaggt aataattcag cataattaat
    16021 taccaggagc agataaacaa tgaagctgat tatttttatt ctcattgttc tgattattgc
    16081 agcgttactt atccggatta tcctcagaag tgtaaatcag cattctcctt tgctgatgca
    16141 attgcatgcg gcaggtatac ggaccggtga tgcagagcga atactgtctg ccgtctggtt
    16201 tttcaggacg gtttttccag tatctgtagc tgctgcgatg aaccccgaac acatggcaga
    16261 gtgtgaccac aggataatgc gctctgagtt tcccgattat cgagaactgt tcagggagtc
    16321 tgacatcaag agcgcggtag ccttttttaa tatttcattc tccatttcaa tgcgttgtag
    16381 ctttttcctc agctcacgta tttcgatttg ttctggtgtt atcggagagg cttttggtgt
    16441 tttgccctga cgctcatcac gcagttgttt gacccatctt gtcattgtgg aaaggccgat
    16501 atccatagct ttggcggcat ctgccaccgt gtagttctgg tcaacaacca gttgagcgga
    16561 ttcgcgttta aactctgcgc tgaaatttct ttttttcatt ggagcacctg tgttgttctg
    16621 aggtgagcat atcacctctg ttcaggtggc caaattcagt gtgccactac atctttcgaa
    16681 aaccagttct gcggcttttc aaagatgcca tcatctctct tcacccgcag gtactggagt
    16741 tcctctggtg gtacaaccat ttgcaatcgc gcttaagatc cagacctggg ccaccgttgt
    16801 catagagtgt ggccttcaag aattctaagt cgccggggtt catgatgttc catttacaat
    16861 gcaatagcgg acggacagtg taaattttta aaattgttcg ctgagcagct ttatcaagcc
    16921 atggattatt tgacgcgccg gtcaataagc tgaccatgcg agtgaaatac ggctgatttc
    16981 tgcgggcgaa ttcggttgcg ttttcagcgc cccactgtgg tagcgtaccg ttcatcttcg
    17041 caatgtagaa acctctgccg atttgtcccg ttggaggaag gaagcgctcc tgaactccga
    17101 cggaaagaat attctgggct gacgcctcac tcgtgccatg ataaccgatg aaatacatat
    17161 ttgatctcaa attattaagt tatattagaa gtttatgcta atactcagga gatatatact
    17221 aaaagcgctc aatgttaata aagtgttatc acaagctact gcccacacca agattgttgg
    17281 ccgttttggt aggtggacga cctcttggac tctcaatata tgagcaacat tcactccttc
    17341 tctcctgagt tttagttgaa atgtgccgca agaatatcag tgaacaatgc caacaatatc
    17401 atttttctat atattgctta tggcattttc ccgcaaagtt tttaatattg agcaaaactc
    17461 tctgtctgcc atcttattta caaataattt gctatctttg tcaattgaat aataatgctc
    17521 gtaactgttt gaaaaatcaa tcggccaaaa acgattattc ttttcatcaa ataagatatt
    17581 tcccatatgt aagtctccat gaatgattac actgtgacgt tgtcgttgag agatatggtg
    17641 ttaccgatag tcaggcatta tataattatg atctgatgac acgaaaaacc attcttacat
    17701 accgcaatca tatcagtgat gatcatatgc ctcttataga ctcagcat
//
LOCUS       DAZGCS010000098        17674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGCS010000098 DAZGCS010000000
VERSION     DAZGCS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.098"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            337..1389
                     /gene="yhiM"
                     /locus_tag="ACHK01_003688"
     CDS             337..1389
                     /gene="yhiM"
                     /locus_tag="ACHK01_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO8098574.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1705..2907)
                     /gene="yhiN"
                     /locus_tag="ACHK01_003689"
     CDS             complement(1705..2907)
                     /gene="yhiN"
                     /locus_tag="ACHK01_003689"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO8098575.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3139..4638
                     /gene="pitA"
                     /locus_tag="ACHK01_003690"
     CDS             3139..4638
                     /gene="pitA"
                     /locus_tag="ACHK01_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO8098576.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4709..5044)
                     /gene="uspB"
                     /locus_tag="ACHK01_003691"
     CDS             complement(4709..5044)
                     /gene="uspB"
                     /locus_tag="ACHK01_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO8098577.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5435..5869
                     /gene="uspA"
                     /locus_tag="ACHK01_003692"
     CDS             5435..5869
                     /gene="uspA"
                     /locus_tag="ACHK01_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO8098578.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5968..6021)
                     /locus_tag="ACHK01_003693"
     CDS             complement(5968..6021)
                     /locus_tag="ACHK01_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098579.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6187..7656
                     /gene="dtpB"
                     /locus_tag="ACHK01_003694"
     CDS             6187..7656
                     /gene="dtpB"
                     /locus_tag="ACHK01_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO8098580.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7705..8457)
                     /gene="rsmJ"
                     /locus_tag="ACHK01_003695"
     CDS             complement(7705..8457)
                     /gene="rsmJ"
                     /locus_tag="ACHK01_003695"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO8098581.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8463..10505)
                     /gene="prlC"
                     /locus_tag="ACHK01_003696"
     CDS             complement(8463..10505)
                     /gene="prlC"
                     /locus_tag="ACHK01_003696"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO8098582.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10708..11550
                     /gene="rlmJ"
                     /locus_tag="ACHK01_003697"
     CDS             10708..11550
                     /gene="rlmJ"
                     /locus_tag="ACHK01_003697"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO8098583.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11622..12974
                     /gene="gorA"
                     /locus_tag="ACHK01_003698"
     CDS             11622..12974
                     /gene="gorA"
                     /locus_tag="ACHK01_003698"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO8098584.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13028..13111)
                     /gene="dinQ"
                     /locus_tag="ACHK01_003699"
     CDS             complement(13028..13111)
                     /gene="dinQ"
                     /locus_tag="ACHK01_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO8098585.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13620..13973
                     /gene="arsR"
                     /locus_tag="ACHK01_003700"
     CDS             13620..13973
                     /gene="arsR"
                     /locus_tag="ACHK01_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO8098586.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14027..15316
                     /gene="arsB"
                     /locus_tag="ACHK01_003701"
     CDS             14027..15316
                     /gene="arsB"
                     /locus_tag="ACHK01_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO8098587.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15329..15754
                     /gene="arsC"
                     /locus_tag="ACHK01_003702"
     CDS             15329..15754
                     /gene="arsC"
                     /locus_tag="ACHK01_003702"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO8098588.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15877..16173)
                     /locus_tag="ACHK01_003703"
     CDS             complement(15877..16173)
                     /locus_tag="ACHK01_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8098589.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16281..17282
                     /locus_tag="ACHK01_003704"
     CDS             16281..17282
                     /locus_tag="ACHK01_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8098590.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         4113 a         4530 c         4416 g         4615 t
ORIGIN      
        1 atgtctgaaa gacatattta cgatgataca aagcaataat acttatatgt tcgatgattt
       61 taagaaccga gactatccgg tcttttacta aataatggaa aacaatccat ccctggagga
      121 taattgtaaa aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg
      181 gccttttaac cggtaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg
      241 catttgtctt taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac
      301 atttcattat tagcagggat acctgaggag tttaaagtga acatatatat cgggtggctt
      361 ttcaaattaa tccctttgat tatgggatta atttgcatcg cgttaggtgg ctttgtgctg
      421 gaaagttcag ggcaaagcga gtatttcgtc gcgggtcatg tgctgatttc tctggcggcc
      481 atatgcctgg cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt
      541 aatacgtttt acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg
      601 atatggggtt gggcactgtt agcaggcaat gatgtgatgg cagacgagtt tgtcgccggc
      661 catgttattt tcggcgttgg tatgattgcc gcctgtgtat cgacggtggc agcgtcatcc
      721 ggtcactttc tgctcattcc caaaaatgca gcggggagca agagcgatgg aacgccggta
      781 caggcttatt cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg
      841 ctcggtttca tttggtctat tacgctgtta cgtagtgctg acataactcc gcattatgtc
      901 gcgggtcacg tattgcttgg gttaaccgca atctgtgcct gtctaattgg ccttgttgcc
      961 acaattgtcc atcaaacacg taatacgttt tcaactaaag aacactggct gtggtgttat
     1021 tgggttattt ttctcggctc aatcacggta ctgcagggga tatacgtctt agtcagttcc
     1081 gatgcaagcg cccgactggc tcccggcatt attcttattt gcctcggaat gatctgttac
     1141 agcatattct caaaagtctg gctactggca ctggtatgga gacgtacctg ttcgttagcc
     1201 aacagaatac cgatgattcc cgtcttcacc tgcctgtttt gccttttcct ggcatcgttt
     1261 cttgcggaaa tggcgcagac cgacatggga tattttattc cttcgcgggt tttggtcggt
     1321 ttaggagcgg tatgctttac gttgttctca atcgtttcaa tattagaagc aggttctgct
     1381 aaaaaataat tgcaacgtac cggataaaac cagcgttgac catttgcgta acgctggttt
     1441 ttcttaggca tcatgaaata acgcagcatt aatgcatagt ggttaaggat aaaaaaacaa
     1501 caaagttact gtttttttca acctgttcat attttataaa gttctggcca ctgggtgagt
     1561 tttcaatcac ctttccttcc accttatatt aagcctggag ggtttcagtt cacgggctca
     1621 ttagaaaata atcacaagat agccactatc aaaattagct catttttaat acggctctaa
     1681 taattttcat ctttaggtaa caggtcagga cgacttcgct gcaatcaaat cctgcgcaca
     1741 agcccacgca ctcgaccacg cccactggaa gttatagccc cccagccagc cggtaacgtc
     1801 catcacttcg ccgatgaagt acagcccagg cactttgcgc gcttccatcg tccgtgaaga
     1861 gagttcgttg gtgtccacgc cgccgagcgt cacttcggca gtgcgatagc cttcagtgcc
     1921 gttgggttgt acgcgccagt cggtcaatgt gctaatcagt gcctgttggt cacgcacgtt
     1981 gagctgtttt agcgaaacat ccgggatttg cccgagttgc tgtaagcgtt caaccaaccg
     2041 cttcggtaga tgaaccgcca gtgtgttttt caggctctga ttcggatgtg cgttacgctg
     2101 ctcattcagg aaggtttcga gatccacatc cggtagcaga ttgatactga caaattcacc
     2161 cggttgccag tagctggaaa tctgcaacac cgccggtcca gacaagccgc gatgggtgaa
     2221 gagtaagttc tcacggaaaa cgatgccgtt ttcagcggta atcacggaag gcaccgccac
     2281 gcccgccagc acctgtaact cttcgagcaa cggtttatgc agagtgaatg gcaccagacc
     2341 cgcgcgggtc ggcagcacgt tgaggccaaa ttgttcggca atcttataac caaacggcga
     2401 cgcgcccagc cccggcattg acagcccacc ggtcgcgatg accagctttt cgcaaccgac
     2461 agtcatgccg ttcagttcaa gcgtgaagcc tgtttcatcc ttcgccacac tcagcacttc
     2521 gctacgcaat ctgaaggtta cattgccctt ctcgcactca tccaccagca tgtcgacaat
     2581 ctgctgcgcg gagtcatcgc agaagagttg tcctaacgtt ttctcgtgcc aggcgatgcc
     2641 gtgtttattg accagatcaa tgaaatccca ctgggtaaaa cgcgcgagtg cagacttaca
     2701 aaaatgcgga ttctggctca gataagcgcc tggttcgaca taaaggttgg taaagttgca
     2761 gcgcccaccg ccagacataa ggattttgcg ccctggtttt ttaccattat cgatcagcag
     2821 aacccggcgt cctgcctgac ctgccagcgc agaacagaac atacccgccg caccagcgcc
     2881 tataataatg gcatcaaacc tttccacgtt gcgctcctct tagaaaaaac gggcgtgaat
     2941 tgtaaagatt cctcagtggt cgcaccagca tcaatattac taaaaggaag tatttgcctg
     3001 aattatataa gataattatt ttttgagtga aatccataca ggaggcaaat caaaaaaagt
     3061 ctatatttca ctttgcccgc gccgcgaaag tcactgataa tgcgccgcgt tcatgtcctc
     3121 aaaatggcgt aacgtcctat gctacatttg tttgctggcc tggatttgca taccgggctg
     3181 ttattattgc ttgcactggc ttttgtgctg ttctacgaag ccatcaatgg tttccatgac
     3241 acagccaacg ccgtggcaac cgttatctat acccgcgcga tgcgttctca gctcgccgtg
     3301 gttatggcgg cggtgttcaa ctttttgggt gttttgctgg gtggtctgag tgttgcctat
     3361 gccattgtgc atatgctgcc gacggatctg ctgcttaata tgggatcgtc tcatggcctt
     3421 gccatggtgt tctctatgtt gctggcggcg attatctgga acctgggtac ctggtacttt
     3481 ggtttacctg catccagctc tcatacgctg attggcgcga tcatcgggat tggtttaacc
     3541 aatgcgttga tgaccgggac gtcagtggtg gatgcactca atatcccgaa agtattaagt
     3601 attttcggtt ctctgatcgt ttcccctatt gtcggcctgg tgtttgctgg cggtctgatt
     3661 ttcttgctgc gtcgctactg gagcggcacc aagaaacgcg cccgtatcca cctgacccca
     3721 gcggagcgtg aaaagaaaga cggcaagaaa aagccgccgt tctggacgcg tatcgcgctg
     3781 atcctttccg ctatcggcgt ggcgttttcg cacggcgcga acgatggtca gaaaggcatt
     3841 ggtctggtta tgttggtatt gattggcgtc gcgccagcag gcttcgtggt gaacatgaat
     3901 gccactggct acgaaatcac ccgtacccgt gatgccatca acaacgtcga agcttacttt
     3961 gagcagcatc ctgcgctgct caaacaggct accggtgctg atcagttagt accggctccg
     4021 gaagctggcg caacgcaacc tgcggagttc cactgccatc cgtcgaatac cattaacgcg
     4081 ctcaaccgcc tgaaaggtat gttgaccacc gatgtggaaa gctacgacaa gctgtcgctt
     4141 gatcaacgta gccagatgcg ccgcattatg ctgtgcgttt ctgacactat cgacaaagtg
     4201 gtgaagatgc ctggcgtgag tgctgacgat cagcgcctgt tgaagaaact gaagtccgac
     4261 atgcttagca ccatcgagta tgcaccggtg tggatcatca tggcggtcgc gctggcgtta
     4321 ggtatcggta cgatgattgg ctggcgccgt gtggcaacga ctatcggtga gaaaatcggt
     4381 aagaaaggca tgacctacgc tcaggggatg tctgcccaga tgacggcggc agtgtctatc
     4441 ggcctggcga gttataccgg gatgccggtt tccactactc acgtactctc ctcttctgtc
     4501 gcggggacga tggtggtaga tggcggcggc ttacagcgta aaaccgtaac cagtattctg
     4561 atggcctggg tgtttaccct tccggctgcg gtactgcttt ccggcggact gtactggctc
     4621 tccttgcagt tcctgtaatc gtacacacca aaacgagcgg gtcagtttac tggcccgctt
     4681 tttttatgcg ttattattgc tgaagtgctt aatgccaaat catcaatgca atcaggctga
     4741 ccaccaccag accacacaat gcgctggtca gaataaactg ccgacgcacc cgctcacagc
     4801 ggcgaataaa ttcatcatcg tgatgatcgc gataacgttg ggcatagata taccaaacga
     4861 gacgcacctg tttgttgggt tggccatgtg aggtaaaaaa gccccctcca tcaacatatt
     4921 gatagagcaa tggatcgcag ttacgcagta ccactaacaa cgcgcgtagt gatgagaaat
     4981 agcgcgccat gttaacaatg caaacgacac ataaagccca aaataatgcg acggtgctta
     5041 tcatacctcc tccccggcga cctgcccgcg gagttccacc ccggggctac cgctcccgat
     5101 acgctgccaa tcagttaaca ccaggtcctg gagaaaccgc ttttgtggtg accaacatcc
     5161 gagcggctct atagatagtg taggagatca ggttgttttt tttccagaag gttaaccact
     5221 atcaatatat tcatgtcgaa aatttgttta tctaacgggt aagcaaggcg gattgacgga
     5281 tcatccgggt cgctataagg taaggatggt cttaacactg aatctttacg gctgggttag
     5341 ccccgcgcac gtagttcgca ggacgcgggt gacgtaacgg cacaagaaac gctagctggc
     5401 cagtcatcga caactttatg gaaggagtaa cactatggct tataaacaca ttctcatcgc
     5461 ggtcgatctc tccccggaaa gcaaagttct ggtagagaaa gcagtctcta tggctcgccc
     5521 ctacaatgcg aaagtttctc tgatccacgt agatgtaaac tactctgacc tatacaccgg
     5581 gcttattgat gtgaatctgg gtgatatgca gaaacgcatc tctgaagaga cacatcatgc
     5641 gctgaccgag ctttccacta atgcaggcta cccaatcact gaaaccctga gcggcagcgg
     5701 cgacctgggc caggttctgg tcgatgcaat caagaaatac gatatggatc tggtggtttg
     5761 tggtcaccac caggacttct ggagcaaact aatgtcttcc gcacgtcagc tgatcaacac
     5821 cgttcacgtt gatatgctga ttgttccgct gcgcgacgaa gaagaataat catcccccca
     5881 cgacgtgttc ctgaacgccc gcatatgcgg gcgttttgct ttttggcgcg ccttgttacc
     5941 tgatgcagcg taaacccctt atctggccta cgatctgcgt acgcaatcaa aatctcctgc
     6001 caatacaaca tttaacacca tcatattttc catcattagt gtgatcagct ggttattttc
     6061 tgttgtaata gtgtattaat ctattcacca catcaatatt aagaatctct gacagatgta
     6121 aactttttcg cgcgttatcc cttacgcgtt catacttttc aggatggtat tggaaggtta
     6181 ataaatatga atacaacaac acccatgggg atgctgcagc aacctcgccc atttttcatg
     6241 atcttttttg tcgagttatg ggagcgattc ggctactacg gcgtgcaggg cgtactggcg
     6301 gttttcttcg ttaaacagct tggattctcg caagagcagg cttttgtcac ttttggtgct
     6361 tttgctgcgc tggtctatgg cctcatttcc attggcggct atgtcggcga ccacctgctg
     6421 gggaccaaac gcaccattgt tctcggagca cttgtgctgg cgattggcta cttcatgacc
     6481 ggcatgtcgc tacttaagcc tgacctgatt ttcatcgccc tggggactat cgctgtcggt
     6541 aacggcctgt ttaaagctaa cccagccagc ttgctttcga agtgctatcc gccgaaagat
     6601 ccgcggcttg atggcgcatt caccctgttc tatatgtcga tcaatatcgg ctcgttgata
     6661 gcgttatcgc tggcccctgt gatcgctgat agattcggtt attcagtcac ctacaacctg
     6721 tgcggggcgg ggttaattat cgcattactg gtttacatcg cctgtcgtgg aatggtgaaa
     6781 gacattggtt ctgaacccga cttccggcca atgagcttca gcaaactgtt gtacgtgtta
     6841 cttggcagcg tggtgatgat cttcgtctgt gcatggctga tgcacaacgt agaagtcgcc
     6901 aatctggtgc tgattgttct ctccatcgtc gtcaccatca tcttctttcg tcaggcattc
     6961 aagctggata aaaccgggcg caataaaatg tttgtcgcct ttgtcctgat gctcgaagcg
     7021 gtggtgtttt acattctcta cgcccagatg ccaacgtcgc tgaacttctt tgccatcaac
     7081 aacgtgcatc atgaaattct cggcttttcc atcaacccgg tcagcttcca ggcgcttaac
     7141 ccgttctggg tggtactcgc cagcccaata ctggcaggca tttacacgca tctgggtaac
     7201 aaaggcaaag acctctcgat gccgatgaaa tttactctcg gcatgtttat gtgctcactg
     7261 ggctttttga cggcggcagc tgcgggaatg tggtttgctg atgcacaagg actgacatcg
     7321 ccgtggttta tcgtgctggt ttacttgttc cagagcctgg gtgaattgtt tattagcgcc
     7381 ctgggtctgg cgatgattgc tgccctggtg ccgcagcatt tgatgggctt tattctcggg
     7441 atgtggttcc tgacgcaggc tgccgcgttc ttgctgggcg gctatgtggc aacatttacc
     7501 gcggtgccgg acaacattac cgatccgctt gagacgttgc ccgtctatac caacgtgttt
     7561 ggtaagattg gtctggtcac gctgggcgtt gcagtagtga tgctgttgat ggtgccgtgg
     7621 ctgaaacgca tgattgcgac gccggaaagc cattaattat tcttgcagaa agcagggtag
     7681 cgttatcgct accctgcttt acttttacac cggcgttccg gcataaatat caaacctatg
     7741 ccctttagtg actaccgcgt ttggcgtggc gacattcgcc agcggtggcg cgtagtccgg
     7801 gcgctttacc accacgcgtt tggtcgccag cagccgcgca ggctccagca atccatcggc
     7861 atcaagatcc ggccccacca gcgactgaaa gacacgcatc tcttttttca ccagcgcgct
     7921 tttctgctta tgcgggaaca tcgggtcgag ataaaccacc tgcgggcgcg gggtaatatc
     7981 agtcagcgcc gtcaggctgg aggcgtgaat taactgcaac cgctcctgca accagccgcc
     8041 gatttccgca tccgcataac cacgcgccag gccgtcgtcg agcaaggcgg caacgactgg
     8101 attacgctcc agcatccgca cgcggcaacc gactgaagcc agcacaaagg catcgcgccc
     8161 cagccctgcg gtggcgtcca ccacatccgg caaataatcg cctttaatgc ccaccgcttt
     8221 cgccaccgcc tcaccgcgac caccgccgaa tttgcgtcgg tgcgccatcg ctccgccaac
     8281 aaaatcaaca aagatgccgc caagttttgg ctcatcacgc ttgcgcaatt ccagatgttc
     8341 cggcgttaac accagcgcca tcaggttgtc ttcatcgtgc tccagccccc agcgggccgc
     8401 cagaacagat aaggcaccgt ctccggtgcc tgtttcatca attaagcaaa tctgcacaaa
     8461 aactaacctt tgatcccgta atgctccagc atcgcatcca gctgcggctc acgaccacgg
     8521 aagcgtttga acagatccat cggctcctct gaaccgccac ggctcagaat gttgtcgagg
     8581 aacgactgcc cggtttcacg gttgaaaatg ccctcttcct caaagcgcga gaaagcatct
     8641 gccgccagta cgtcagccca caggtagctg tagtaacctg cggcataacc accggcgaaa
     8701 atatggctga aagcgtgcgg gaaacggccc caggatggag acggtactac ggcaaccagt
     8761 ttcttgattt ctgccagagt ttcgaggatt ttcgctccct gatccgggcg gaactcggca
     8821 tggaggcgga aatcgaacag gccgaactcc agctggcgca gaataaacag cgccgcctgg
     8881 tagttcttcg ccgccagcat tttatccagc aactctttcg gcagcggttc gccggtttcg
     8941 tagtgaccag agataaacgc cagcgcctca ggctcccagc accagttttc cataaactga
     9001 ctcggcagtt cgaccgcatc ccacggcacc ccactgatcc cggatacacc ggcggtttcg
     9061 atgcgggtca gcatatgatg cagaccatga ccgaactcgt ggaacagggt gatcacttcg
     9121 tcatgggtaa acagcgccgg tttaccattt accgggcggt taaagttaca ggtcagatac
     9181 gcgaccggtt tttgcagaga accgtcagct ttgcgcatct ggcctacgca gtcatccatc
     9241 cacgccccgc cgcgcttgtt ttcacgggca tacaggtcga ggtagaagct accgcgcagt
     9301 tcgttgttct cgtcatacag ttcgaagaaa cgtacatccg ggtgccagac atcaacatct
     9361 ttacgctctt tagcggtgat gccgtaaata cgtttcacca cttcaaacaa accgttaacc
     9421 gctttgtttt ccgggaagta cggacgcagc tgttcgtcac tgatgctgta gaggtgctgt
     9481 ttttgttttt cgctgtagta cgcgatatcc cacggctgca actcatcgac gccaaattcg
     9541 gctttggcaa aggcgcgcag ttgtgccagc tctttttcgc cttgcggacg cgcgcgtttt
     9601 gccagatcgg ttaagaaatc cagcacctgc tgcgggtttt ctgccatttt agtggcaagg
     9661 gatttaaagg cgtagttttc aaagcccagc agttgcgcca gttcgtgacg cagcgcgagg
     9721 atctcttcca tcaccttgct gttgtcccac ttaccggcgt tcggaccttg atcggaggcg
     9781 cgggtgctgt aagcgcgata catctcttca cgcagagcct ggttgtcgca gtaggtcatt
     9841 accggcaggt agcttgggat atccagcgtc agcaggtagc cttcaagttc tttcgcttca
     9901 gcctgggctt ttgccgcagc cagcgcgctt tctggcatcc ccgccagttc cgcttcgtcg
     9961 gtaacgagtt tggtccagcc cattgtcgca tcgaggacgt tgttgctgta ctggttgccc
    10021 agttcagaaa gacgggtagc aatttcgccg taacgctgct gtttctcttt cggcagacct
    10081 atgccagaga gttcaaaatc acgcagggcg ttatcaaccg ctttcttctg cgccgtgttc
    10141 agcgtggcgt aatgatcgcc atcgcgcagg tcgcggtacg ctttatacag cccttcatgt
    10201 tgccctaccc aggtgctgta ttccgacagc agcggcaggg tttgttcgta cgcttcacgc
    10261 agttccgggc tatttttcac cgagttcagg tggctgaccg gggagaagat acgccccaac
    10321 acatcgtcca cttccgccaa cggctggcag agattttccc aggtgtacgg tgccccttgc
    10381 gctactacgc gctccacgtt ttcgcggcag tcgttcaatg ccttagtcac ggctggaacg
    10441 acatgttccg ggagaatttt agaaaacgga ggcaattcaa agggagtcag taacggattc
    10501 gtcattcgcg cagtcctggt taaagaggtt aaggaagcgc tcaacaggcg ctttacataa
    10561 tgtgtgtagc atggggttaa gtgtagtgaa tttcaatgag aaacgttacg ctttcgcggc
    10621 ggcggcctct tttcggtata ctgtcctgat acgcttttgt gcgccccgaa tacgggccga
    10681 tttttactta ccggaacacc tttacccatg ctcagttatc gccacagctt tcacgctggc
    10741 aaccacgccg acgtccttaa acataccgtt cagagcctga tcatcgagtc gctgaaagag
    10801 aaagataaac cgtttctcta tctcgacacc cacgcagggg ccgggcgtta tcagttaggc
    10861 agcgaacatg ccgagcgtac cggcgaatat ctcgaaggca tcgcccgtat ctggcagcag
    10921 gacgatttgc ccgcagaact ggaggcgtac atcaatgtgg taaaacactt caaccgtagc
    10981 ggtcagttgc gttactaccc cggttcgccg ttgattgctc gccagctact gcgcgaacaa
    11041 gacagcctgc aactgaccga actgcacccg agcgattacc cgttgctgcg ttctgaattt
    11101 cagaaagaca gccgtgcgcg cgtcgaaaaa gccgatggtt tccagcagct taaggccaaa
    11161 ctgccgccgg tttcccgccg tggtttaatc cttatcgacc cgccgtatga aatgaaaacc
    11221 gactatcagg cggtggtcag cgggatagca gaaggttaca aacgtttcgc cactggtact
    11281 tacgcactgt ggtatccggt ggtgctgcgt cagcaaatta agcgcatgat ccacgatctg
    11341 gaagcgaccg gtattcgcaa aattctgcaa attgaactgg cggtactgcc agacagcgat
    11401 cgccgtggca tgaccgcttc cggcatgatt gtgattaacc cgccgtggaa actggaacaa
    11461 cagatgaata acgtgctgcc gtggctgcac agcaaactgg ttccggcagg caccgggcac
    11521 gccaccgtaa gctggatcgt gccggagtaa ttgcagccat tgctggcacc tattacgtct
    11581 cgcgctacaa tcgcggtaat caacgataag gacactctgt catgactaaa cactatgatt
    11641 acatcgccat cggcggcggc agcggcggta tagcctccat caaccgcgcg gctatgtacg
    11701 gccagaaatg cgcactgatt gaagccaaag agctgggcgg cacctgcgtg aacgtcggct
    11761 gtgtgccgaa aaaagtgatg tggcacgcgg cgcaaatccg tgaagcgatc catatgtacg
    11821 gcccggatta tggttttgat accactatca ataaattcaa ctgggaaacg ttgatcgcca
    11881 gccgtaccgc ctatatcgac cgtattcata cttcctatga aaacgtgctc ggtaaaaata
    11941 acgttgatgt aatcaaaggc tttgcccgct tcgttgatgc caaaacgctg gaggtaaacg
    12001 gcgaaaccat cacggccgat catattctga tcgccacagg cggtcgtccg agccacccgg
    12061 atattccggg cgtggaatac ggtattgatt ctgatggctt cttcgccctt cctgctttgc
    12121 cagagcgcgt ggcggttgtt ggcgcgggtt acatcgccgt tgagctggcg ggcgtgatta
    12181 acggcctcgg cgcgaaaacg catctgtttg tgcgtaaaca tgcgccgctg cgcagcttcg
    12241 acccgatgat ttccgaaacg ctggtcgaag tgatgaacgc cgaaggcccg cagctgcaca
    12301 ccaacgccat cccgaaagcg gtagtgaaaa atgccgatgg tagcctgacg ctggagctgg
    12361 aagatggtcg cagtgaaacg gtggattgcc tgatttgggc gattggtcgc gagcctgcca
    12421 atgacaacat caacctggaa gccgctggcg ttaaaactaa cgaaaaaggc tatatcgtcg
    12481 tcgataaata tcaaaacacc aatattgaag gtatttacgc ggtgggcgat aacacgggtg
    12541 cagtggagct gacaccggtg gcagttgcag cgggtcgccg tctctctgaa cgcctgttta
    12601 ataacaagcc ggatgagcat ctggattaca gcaacattcc gaccgtggtc ttcagccatc
    12661 cgccgattgg tactgttggt ttaacggaac cgcaggcgcg cgagcagtat ggcgacgatc
    12721 aggtgaaagt gtataaatcc tctttcaccg cgatgtatac cgccgtcacc actcaccgcc
    12781 agccgtgccg catgaagctg gtgtgcgttg gatcggaaga gaagattgtc ggtattcacg
    12841 gcattggctt tggtatggac gaaatgttgc agggcttcgc ggtggcgctg aagatgggtg
    12901 caaccaaaaa agacttcgac aataccgtcg ccattcaccc aacggcggca gaagagttcg
    12961 tgacaatgcg ttaaatgtta aagggctaag agtagtgtgc tcttagccct taattacgtt
    13021 tccgctatca gttcagaagc tgaagcagaa agcggatcag ttccagcagc gcaattaacg
    13081 ccccaagaac gatgattgct ttatcaatca cccgttttct ccatgcgatg gagtgagaat
    13141 gcatccgctt actcatccac tgcctgtcac ggcgcatgcc ctattgttag ataatggcat
    13201 tctcactccg gccagagcat cagttaacgg caccacccgt acttctgacc aggactttga
    13261 aagcgtttat gcgcattacc agagtgaaaa tgcctcagag ctagctggat aatcatacag
    13321 tacattcagg ttataaaacc agcatgtcct tgcaatagtt tcagtatggt attagcattg
    13381 atgcgttaga taatggcatt ctcactccag ccagtgccac caactcaggg ctggaaagta
    13441 aaaaaccgac gcaaagtcgg tttttttacg tcctgattca gacctccttt caaatgaata
    13501 gccaactcaa aattcacacc tattaccttc ccctgcactt acacattcgt taagtcatat
    13561 atgtttttga cttatccgct tcgaagagag acactacctg caacaatcag gagcgcaata
    13621 tgtcatttct gttacccatc caattgttca aaattcttgc tgatgaaacc cgtctgggca
    13681 tcgttttact gctcagcgaa ctgggagagt tatgcgtctg cgatctctgc actgctctcg
    13741 accagtcgca gcccaagatc tcccgccacc tggcattgct gcgtgaaagc gggctattgc
    13801 tggaccgcaa gcaaggtaag tgggttcatt accgcttatc accgcatatt ccagcatggg
    13861 cggcgaaaat tattgatgag gcctggcgat gtgaacagga aaaggttcag gcgattgtcc
    13921 gcaacctggc tcgacaaaac tgttccgggg acagtaagaa catttgcagt taaaaattta
    13981 gctaaacaca tatgaatttt cagatgtgtt ttatccggga ggcattatgt tactggcagg
    14041 cgctatcttt gtcctgacca tcgtattggt tatctggcag ccgaaaggtt taggcatcgg
    14101 ctggagtgca acgctcggcg cagtactggc gttagttacg ggcgtggtcc atccgggtga
    14161 tattccggtg gtgtggaata tcgtctggaa cgcgacggct gcgtttatcg ccgtcattat
    14221 catcagcctg ctgctggatg agtccggctt ttttgaatgg gcggcgctgc acgtctcacg
    14281 ctggggtaat ggtcgtggtc gcttgctgtt tacctggatt gtcctgctcg gtgctgccgt
    14341 tgccgccctg tttgccaatg atggcgcggc gcttattttg acaccgattg tcatcgccat
    14401 gctgctggct ttagggttca gtaaaggcac tacgctggcg ttcgtgatgg cggccggatt
    14461 cattgccgat accgccagcc tgccgcttat tgtctccaac ctggtgaata tcgtttccgc
    14521 tgatttcttt ggcctcggct ttcgcgaata cgcctcggtg atggtgccgg tggatatcgc
    14581 cgcgattgtt gccacgctgg tgatgttaca tctctatttt cgcaaagata ttccgcagaa
    14641 ctacgatatg gcgctgctga aatctcccgc agaagcgatc aaagatcctg ctacgttcaa
    14701 aactggctgg gttgttttac tgcttctgct ggtgggattt ttcgtcctgg aaccgctcgg
    14761 cattccggtg agcgccattg cagctgtggg cgcgctgata ttatttgtcg tcgctaaacg
    14821 cggtcatgcg attaatacgg gtaaagtcct gcgcggtgcc ccctggcaga ttgtcatctt
    14881 ctcgctcggc atgtatctgg tggtttatgg cctgcgcaat gccggattaa cggaatatct
    14941 ttctggcgta ctcaacgtgc tggcggataa cggcctgtgg gccgcgacgc tcggcaccgg
    15001 attcctcacc gccttcctct cttctattat gaacaatatg ccgacggtac tggttggcgc
    15061 gttgtccatt gatggcagca cggcatctgg cgttatcaaa gaagcgatgg tttatgccaa
    15121 tgtgattggc tgcgatttgg gaccgaaaat taccccaatt ggtagcctgg ctacgctact
    15181 ctggctgcac gtactttcgc agaagaatat gactatcagc tggggatatt acttccgtac
    15241 agggattatc atgaccctgc cggtgctttt tgtgacgctg gctgcgctgg cgctacgtct
    15301 ctctttcact ttgtaatgag atattgatat gagcaacatt accatttatc acaacccggc
    15361 ctgcggtacg tcgcgtaata cgctggagat gatccgcaac agcggtacag aaccgaccat
    15421 tatctattat ctggaaacac cgccaacgcg cgatgagctg gtcaaactca ttgccgatat
    15481 ggggatttcc gtacgcgcgc tgttgcgtaa aaacgtcgag ccgtatgagg aactgggcct
    15541 tgcagaagat aaatttactg acgatcggtt aatcgacttt atgcttcagc acccgattct
    15601 gattaatcgc ccgattgtgg tgacgccgct gggaactcgc ctgtgccgcc cttcagaagt
    15661 ggtgctggaa attctgccag atgcgcaaaa aggcgcattc tccaaggaag atggcgagaa
    15721 agtggttgat gaagcgggta agcggttgaa ataaagcggc gatatccact gcccatagtt
    15781 ggtaaagtgc gctttgttta tgtcggatgc gacgctaacg cgtcttatcc gacctacaaa
    15841 accgtgcatg gcatgaaatc tgtagcgcgt agcgcatcag gcaattttgc tatttacgca
    15901 gcactcctcg cgtaagaatt caattatcgc ttccagcatt gcgtattgcg aaacacacat
    15961 cagcgtccga ctctcacggt tttgtttaac cagccctacc gataccagtg ctgaaatatg
    16021 gtgactgagt gtagagccgg gtatcccaag ctgcttttgc aactcaccga ccggcaaacc
    16081 ttgctccccc gctttgacca ggtgtttgaa tatgaacaaa cgggtcgggt gacctagctc
    16141 tttcagcgct ttagcaactt cttccaactg catatgacac cccctttgtc tggttgatat
    16201 ttcgataata ccagaaatat cttgatctga atttcatttc gattattctg gaaatataga
    16261 tttatccaat gaggttacgt atgagtagtt ggcttgcgat gctgcaagat gccgcagaga
    16321 tgtttgtgtt tctcgccgtc gagctttcgt tgctgtttat agtgattagt gccggtgtca
    16381 gcctgataag acaaaaggtg ccagaccata aaatccagca gatgatgggg gccagaaaag
    16441 ggagaggcta tctcctggct gctctgttgg gagccgttac cccgttctgt agttgctcga
    16501 caatacccat gttacgtgga ttgttgtcag cgaaagccgg gtttggtccg actctcactt
    16561 ttttatttgt ttcaccatta cttaatccca ttatcgtcgg actaatgtgg atgacctttg
    16621 gttggaaagt caccttgttg tacgcgatta tcgccgccgg cgtctccgtt cttgccagta
    16681 ttatcctgga ttctctgggg tttgaacgtc atatcattgc cagtaaaagc tcatcagcaa
    16741 attgttgtgc tctagcaaaa acttcgccgg gaacgacata tacgcccata aaagtgagtt
    16801 gttgtagtcc agcggctaaa gccattgaga accccgtagt tacctgttgc agtaccaaag
    16861 ctgtagtaag tattaatccc ataaaactag ccaccaaaga tgcgttgcaa cagtttaaag
    16921 atgtactgcc atatcttttg ttaggagtat taataggatc ttttatttat ggctttattc
    16981 cctcagcgtg gattgccgct catgcaggag cagataatcc cttcgccatc ccattaagcg
    17041 ccgttgtcgg tattccgctg tatatccggg ctgaggcagt tatccctctg gcatctgttc
    17101 tgatgacaaa aggaatgggt ctgggagcat taatggcatt aatcatcggc agtgccggcg
    17161 caagcctgac ggaagtgata ttacttaaat caatgttcag aatgccgatg atagctgcat
    17221 tcctgacggt tatattaggt atggctatct tgatgggcta tttgactcaa ttgctatttt
    17281 aaatagatgc cagcagtaaa ctataagatg catagttagt gccatatcct tctaagtaga
    17341 aaatcataga caggataatc cttaaaaatc acaaatgatt taactaatgc attgagaatg
    17401 atgacggctc ggtcaacaag tcatcaacag acaataacca cgtaatcatg cttctctgac
    17461 attttatcgg agaagcaatg actctttgtc tcagtaccct cctccttttt aaaaggcaga
    17521 tttcagtcgc taatacaaca catttttaaa tacactttaa cctggttcat tattacacat
    17581 ctaaaacatc acaaaaatat tacttcacac actatcggag aacataataa ctttttaaaa
    17641 tgagcgcttt ttgatagttg ttaacgtttt cctt
//
LOCUS       DAZGCS010000099        17448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGCS010000099 DAZGCS010000000
VERSION     DAZGCS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.099"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="ACHK01_003705"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="ACHK01_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO8098591.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="ACHK01_003706"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="ACHK01_003706"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO8098592.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1606..1719
                     /locus_tag="ACHK01_003707"
     CDS             1606..1719
                     /locus_tag="ACHK01_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO8098593.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            1980..2546
                     /gene="yceJ"
                     /locus_tag="ACHK01_003708"
     CDS             1980..2546
                     /gene="yceJ"
                     /locus_tag="ACHK01_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8098594.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2550..3125
                     /gene="yceI"
                     /locus_tag="ACHK01_003709"
     CDS             2550..3125
                     /gene="yceI"
                     /locus_tag="ACHK01_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO8098595.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3167..4219)
                     /gene="trhO"
                     /locus_tag="ACHK01_003710"
     CDS             complement(3167..4219)
                     /gene="trhO"
                     /locus_tag="ACHK01_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO8098596.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4444..5364
                     /gene="lpxL"
                     /locus_tag="ACHK01_003711"
     CDS             4444..5364
                     /gene="lpxL"
                     /locus_tag="ACHK01_003711"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO8098597.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5536..6762
                     /gene="mdtG"
                     /locus_tag="ACHK01_003712"
     CDS             5536..6762
                     /gene="mdtG"
                     /locus_tag="ACHK01_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO8098598.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6845..7219
                     /gene="msyB"
                     /locus_tag="ACHK01_003713"
     CDS             6845..7219
                     /gene="msyB"
                     /locus_tag="ACHK01_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8098599.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7220..7447)
                     /gene="yceK"
                     /locus_tag="ACHK01_003714"
     CDS             complement(7220..7447)
                     /gene="yceK"
                     /locus_tag="ACHK01_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8098600.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7620..10163)
                     /gene="mdoH"
                     /locus_tag="ACHK01_003715"
     CDS             complement(7620..10163)
                     /gene="mdoH"
                     /locus_tag="ACHK01_003715"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO8098601.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10156..11691)
                     /gene="mdoG"
                     /locus_tag="ACHK01_003716"
     CDS             complement(10156..11691)
                     /gene="mdoG"
                     /locus_tag="ACHK01_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO8098602.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12085..13242
                     /gene="mdoC"
                     /locus_tag="ACHK01_003717"
     CDS             12085..13242
                     /gene="mdoC"
                     /locus_tag="ACHK01_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO8098603.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13250..14731)
                     /gene="clsC"
                     /locus_tag="ACHK01_003718"
     CDS             complement(13250..14731)
                     /gene="clsC"
                     /locus_tag="ACHK01_003718"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO8098604.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14673..15206)
                     /gene="ymdB"
                     /locus_tag="ACHK01_003719"
     CDS             complement(14673..15206)
                     /gene="ymdB"
                     /locus_tag="ACHK01_003719"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO8098605.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15301..15612)
                     /gene="ymdA"
                     /locus_tag="ACHK01_003720"
     CDS             complement(15301..15612)
                     /gene="ymdA"
                     /locus_tag="ACHK01_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO8098606.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15733..16065)
                     /gene="csgC"
                     /locus_tag="ACHK01_003721"
     CDS             complement(15733..16065)
                     /gene="csgC"
                     /locus_tag="ACHK01_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO8098607.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16124..16579)
                     /gene="csgA"
                     /locus_tag="ACHK01_003722"
     CDS             complement(16124..16579)
                     /gene="csgA"
                     /locus_tag="ACHK01_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO8098608.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16620..17075)
                     /gene="csgB"
                     /locus_tag="ACHK01_003723"
     CDS             complement(16620..17075)
                     /gene="csgB"
                     /locus_tag="ACHK01_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO8098609.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(17204..>17448)
                     /locus_tag="ACHK01_003724"
     CDS             complement(17204..>17448)
                     /locus_tag="ACHK01_003724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098610.1"
                     /translation="FFSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSG
                     KRRFKRKQNIFCVDNTVFTWVLILWYEQKKKNTTRG"
BASE COUNT         4360 a         4440 c         4145 g         4503 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca
      541 tatgccaccg catcaacacg gcagccacca tggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt gggatgaact
      661 ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc
      961 aattcgtcat gacgatgatg gcgtaactat tgaaaccgtt gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg
     1681 ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact
     1741 gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt
     1801 ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca
     1861 ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt
     1921 gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgcattg gatgcactta
     1981 tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa
     2041 gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt
     2101 acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga
     2161 tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga
     2221 gttattcgcc catgactcgt cttgccgcaa aggctggaca tctcgcattg tacctattgc
     2281 tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg
     2341 tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg
     2401 ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat
     2461 ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa
     2521 agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg
     2581 cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag
     2641 gtcagcacgc ctttgttaat ttccgcatcc agcaccttgg ctatagctgg ttatacggca
     2701 cctttaaaga tttcgacggt acttttacct ttgacgaaaa aaatccggct gccgataaag
     2761 tgaatgtgac aattaacacc accagcgtcg atactaatca cgccgaacgc gataaacatc
     2821 ttcgcagtgc agatttcctc aataccgcaa aatatccaca ggcaacattc acctccacca
     2881 gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa
     2941 ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca tggggtggta
     3001 aacgtgcagg cttcgaggcc gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag
     3061 atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga
     3121 agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat tccgttggat
     3181 caggaatgcc cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat
     3241 tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc
     3301 aaatctcact acaacaacct ttgtattttt ccgcacatac tggacactga ataaacagca
     3361 ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc
     3421 aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt
     3481 tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc
     3541 cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt
     3601 cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca
     3661 tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt
     3721 caaagtgccc cacttcatat tcatagtggt tacgcatgtc gataaatagt gcatcgggat
     3781 cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa
     3841 agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg cgcagtaccc
     3901 agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct aaagccgggt
     3961 cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg
     4021 cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct
     4081 gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa
     4141 tggtggttcg cggttcgctc tcagccaaca ttttggcttt tagcgcgtcg ttggaaatgc
     4201 ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg
     4261 catgatatag caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta
     4321 aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg
     4381 ttgcgggcga aaaaatgcga caatacatac aattgcccga ataggttgaa aaacaggatt
     4441 gatatgacga atctacccaa gttctccact gcactgcttc atccgcgtta ttggttaacc
     4501 tggttgggta ttggcgtact ttggttagtc gtgcaattgc cctacccggt tatctaccgc
     4561 ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg
     4621 catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg
     4681 gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggcatggc gtggttctgg
     4741 ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg
     4801 caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggt
     4861 gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca
     4921 ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc
     4981 aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg
     5041 gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt tgagcaggct
     5101 gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc
     5161 gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc gccagagtgt
     5221 tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa
     5281 tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg
     5341 gaaggcgttc cttcacgcta ttaaatctcc cctgccggat gcttcagaat ggcatccggc
     5401 attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga
     5461 agtcgggcgc ataattagtg tgcttatctt ttcttcttat gttcaccgcg cctggcgcac
     5521 caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac
     5581 ctgatcgtcg cctggctagg ctgttttctt accggtgccg ccttcagtct ggtaatgccc
     5641 ttcttacccc tctacgttga gcagcttggc gttaccggtc actccgccct gaatatgtgg
     5701 tccggtattg tcttcagcat tacattttta ttttcggcca tcgcctcacc gttttggggt
     5761 ggactcgccg accgtaaagg ccgaaaactc atgctattac gctctgccct cggcatgggc
     5821 atcgtgatgg tgttgatggg actggcacaa aatatctggc agtttttgat cctacgggcg
     5881 cttcttgggt tacttggcgg atttgtcccc aacgctaatg ctcttatcgc cacacaagta
     5941 ccgcgtaata aaagcggctg ggcgctgggt acgctctcca caggcggcgt tagtggtgcg
     6001 ttgctcggcc caatggctgg cggcctgctc gccgatagct acggcttacg tccggtattc
     6061 tttattaccg ccagtgtgct catactctgc tttttcgtca ccctgttctg catcagagaa
     6121 aaattccagc cggtcagcaa aaaagagatg ctgcacatgc gggaagtggt gacatcactt
     6181 aaaaacccga aactggtact cagcctgttt gtcaccacgt taatcatcca ggtggcgaca
     6241 ggctcaattg cccccattct gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac
     6301 gtcgccttta tcagtggcat gatcgcctcg gtgccaggcg tggcggctct gctaagcgca
     6361 ccacgactcg gcaaacttgg cgatcgaatc ggacccgaaa agatcctgat tacagcgctg
     6421 atcttttctg tactgctgtt gatcccaatg tcttacgttc agacgccatt gcaacttggg
     6481 attttacgtt ttttgctcgg tgccgccgat ggtgcactac tccccgccgt acagacactg
     6541 ttggtttaca actcgagcaa ccagatcgcc gggcgtatct tcagctataa ccaatcgttt
     6601 cgtgatattg gcaacgttac cggaccattg atgggagcag cgatttcagc gaactacggt
     6661 ttcagagcgg tatttctcgt caccgctggc gtagtgttat tcaacgcagt ctattcatgg
     6721 aacagtctac gtcgtcgtcg aataccccag gtatcgaact gatttttcgc ctttcatact
     6781 tgcaaaagcg gagaatcagc tatccttttc cctgaaacct catcaactca aagggagaat
     6841 cgtgatgacc atgtacgcaa cgcttgaaga agccattgac gctgcacgcg aagaatttct
     6901 tgcagacaac cccggcatcg acgccgaaga tgcgaatgtg caacagttca atgcccaaaa
     6961 atacgttttg caggacggcg acatcatgtg gcaagttgag ttttttgccg acgaagggga
     7021 agaaggtgaa tgtttaccta tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga
     7081 ctatgatgag atagagatac gccaggagtg gcaggaagag aatacattac atgaatggga
     7141 cgagggggaa tttcagcttg agccaccgct ggataccgag gaaggacgcg cagcagctga
     7201 tgagtgggat gaacgttaat cactcatacg ggccatgatg aatgtcgatc ggcagcagta
     7261 aagtatcaaa aaccagagag aatggcagat caaggatcgt gacataacgc caggcggagt
     7321 cacgcacatc ccattgcaca ccgggataat attggttgcc atgcccctgc cccggtatag
     7381 tgcgactaat aatgctgcca cagccactca gcaaggtcac catgatgctc accacaatta
     7441 aacgcatttg atacctcttt cgtaaaaaaa taccggcata acgccggtat ttttattggc
     7501 tcgttgtttt tgccggatgc ggcgtgaacg ccttatccga cccacaaaat cttgcaaaat
     7561 caataaattg caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt
     7621 tattgcgaag ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa
     7681 taactcaccc atgaagaata tctctccggg gaattccaga cgcggaaatg cagacgggcc
     7741 atcgtcaccg gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc
     7801 tcgttcagcg cctgttcaac gtggcggtca cgggcgattt ccagcacctt actggcgcgg
     7861 tgacgcgcgg tcgccattgc ggttgccaga gcgttaaatg acgggttaaa cactgcgtgc
     7921 ataaagccat catcaaggga gcgttgacga ttcatctcaa ggaaccgatc ggtatcaacc
     7981 agcacctgcg gcggcgaata ctcttccggg atcaggaaca gtttccagcg tttggtgcgc
     8041 agaccaacgg tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag
     8101 acaatcggtg ccagccagaa caggaaacgc agatccaacc acgccatacc aacagcccac
     8161 actaacccta acagcagctg tgagccgtgg cgtttgaacg cttcacccca ggaagtggag
     8221 tcatcatcac gctgcggtga attccacacc acttcccagc caaggaacgc gctgacgacg
     8281 aagaccgtat ggaacagcat gcgtaccgga gccagcagca cggaaaacag cacttccagc
     8341 agcaacgata atgtaacgcg ccagaagccg ccgtattctt tcgttccttt gcaccagata
     8401 agcaaaatgc tcaataactt cggcaggaac aacagcacca tggtcgaagc aaaaagtgca
     8461 atcgccagct caggacgcca ctgcggccac accgggaaca actgccgtgg ttgcaggaag
     8521 tattgcggtt cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata
     8581 aaccacagcg gagcggagag ataagacatc acgcccgtca ggaataccgc acggtgaacc
     8641 gggtgcatac ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg
     8701 cggtcacgtt ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga
     8761 tcgtaagcaa tccagacccc ccaacctgca cggcgcatca acgccgcttc cacgaagtca
     8821 tgtgacagga ttgaaccggc aaaggaacct tcgcccggca gcggagccag tgcgcagtgc
     8881 tcgataaacg gtttcacgcg gataatcgcg ttatgtcccc agtagtgcga ctcgccaagt
     8941 tgccagaagt gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga
     9001 cagcgcgcat acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg
     9061 ttcgggttgg cttccatcag gcgcaccagg ccgcataaac aatcaccggt cattaccgag
     9121 tcagcatcca gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg
     9181 atattaccgc ttttacgctt cacgcggcga cggcggcggc gatagaaaat ctgaccttcg
     9241 ccaccgactt cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg
     9301 ttataactgt cactaagaat gtagacatca aagtgtttgg cattcccggt ggcttttact
     9361 gattcccacg ttgcacgcag gccagcaaaa acacggttca cgtcttcgtt acagataggc
     9421 atgatcaacg ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat
     9481 atactgtatt tatcgcgacc aataagcagt tgcaggaagc ccattaacgc cgtccagaat
     9541 ccggcggaca cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata
     9601 taaggcagaa gctgcataaa ggaaacccac aaatcctgac caaccatatc cataggatta
     9661 atcagcgccc aaccctgata aggaagaatg gtcttcatat accaggtcgc gacgacagtt
     9721 tgcgcgagcg tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt
     9781 tgctcgctct cctgctcttc tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt
     9841 ccacgcaggc gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc
     9901 gaggagcgtt ttgcttctgg catcgccttc agctgatcgc gcccttcgtc gtctttaatt
     9961 aactgtccat cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct
    10021 tgcggggaat catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg
    10081 gcgcggatat cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca
    10141 atgtactcag ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt
    10201 ctgatcggca ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt
    10261 cacacgcatc accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttcaac
    10321 tatctcacca ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt
    10381 tttcatctct gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcgaat
    10441 caggttcgac tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc
    10501 tggcgcatgc agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc
    10561 tttacccggc tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc
    10621 atcgttggtt ggaatttcta ccagctcaac gctgccttta ccccactccc ctttcggagt
    10681 cacccatgcg cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc
    10741 acgaccgcgc tgcaacagac cgaagccttg cgggttttcc atagagaagc tgctgaccgc
    10801 taaatgtttc gggttattca acggacgcca gatccattcg ccattaccgg catggataga
    10861 gagaccgtta gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg
    10921 cccaaacagg aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag
    10981 atagattttc gactgcacat ccacaaccgt gtcacgccct ggcataacta cgaatttgta
    11041 agcacctgtc gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg
    11101 ttttggacgc tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc
    11161 ggtatcaatt gccaggccgc gggcagaaag gccataaacc tgacctgcac caatcacgcg
    11221 gaaatagctg gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg
    11281 gtaaagcact ttaaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac
    11341 atcgccgaaa gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac
    11401 ttcatttatt ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa
    11461 tggggtcttc agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc
    11521 cgcgtatttc atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt
    11581 cccggctaag gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga
    11641 tgtatacagg gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt
    11701 atgtttcata attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt
    11761 aatggataac cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg
    11821 gattaagcac ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat
    11881 cgacttcgtg tagggtttgc cagcaatttt atttatcaac cgtcttacag acgcttccat
    11941 tctggttagt ggtagcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg
    12001 tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa
    12061 aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc
    12121 gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat
    12181 tcgagccata catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat
    12241 gacttcatcc actcgttccg catgcaggta tttttcgtta tatccggcta cttttcctac
    12301 atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt
    12361 atcccgatgt taacagccat ccccttactg acattgccgc aatttattat gctgcaatac
    12421 gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg
    12481 gcctgggaat taatatcaca tctgtggttt ttactggtgt tagtggtcat gacgacgctg
    12541 tgcgtatgga tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa
    12601 aaattctcga tggtaaaact atcggtgatt tttttatgcc tcggcatcgg ttatgcggta
    12661 ataagaagaa cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt
    12721 attgtcatgc aaacgctgtt ttatttgccg ttctttatcc tcggcgcact ggctttcatt
    12781 ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct tgcagcagca
    12841 ttggcgtttg tcgcttattt actcaaccag cgctatggca gtggcgatgc ctggatgtac
    12901 gaaaccgagt cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc
    12961 tttggccacc gtttgcttaa cttccagtca gcgcgggtga cttattttgt taacgcatcg
    13021 ctgtttatct atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg
    13081 cacatcacct ccaactggct tggttttctc tgtggcctga tatttgtagt agggattgcg
    13141 ataattctgt atgaaattca tttgcgcatc ccgttactga agtttttgtt ctctggtaaa
    13201 ccggttgtta agcgtgagaa cgataaagca ccagcccgtt aagccacatt tacaataacc
    13261 attccacggg caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg
    13321 gttctttttt gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc
    13381 acctgtccag acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa
    13441 ttaactgtgc cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgttg
    13501 aacgcggatc gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaggttt
    13561 tagcatgcag gctggctccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt
    13621 gttcacgcgt cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc
    13681 agcgcgcgta tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga
    13741 tcgcaatctt tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg
    13801 gtacaaaata ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca
    13861 ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac
    13921 ttaataaacg tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa
    13981 acggactgga ttccattttg cgtaagtaac gatgcgtcat agcatcgtta tgccaggagg
    14041 cgggtaactc gatgcgatcc gccatttcac cttccgggac atccagcact tgctgtaagg
    14101 gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg
    14161 gccctattgc cataacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg
    14221 catcaccaat atttcgcccc cctaccagcg tcaccacgcc atcgacagtg aaacttttat
    14281 tgtgcatacg gcgattgaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc
    14341 gaaacgagaa aggattaaaa agccggactt caatgcgggg atggctgtca agcaagcgta
    14401 aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac
    14461 gctttgctgc ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga
    14521 tgtaatactg aacatccagc gtatgctctg ccatttcggc cagacgatac cgggcggcaa
    14581 acgcatccag acttttctcc agagggaaaa gaccacactg accgggatgt tgcgaacaca
    14641 gtggcagcac cgcgctcgcc agccggggca aatcattcat ctccttgttg ggtaaggagt
    14701 ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa
    14761 gcgtgacggg taataaattc tgaaacggtt ttaactgcga tttcagccgc tgccgcacga
    14821 gggtaaccat aaaccccagt actgattgca ggaaaagcca ctgacgtata gctgtttgcc
    14881 gccaccagtc gtaggctatt gagataggca tcctgcaaaa gctggtcttc gttttgttca
    14941 ccaccacgcc agactggccc gacggtgtgc actacggctt tagcgggaag agcgcctgca
    15001 agcgtaataa cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacag
    15061 gcatccagca gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc
    15121 attaatgacg gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc
    15181 tgcacaacat gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt
    15241 acagcaggct ttttggttaa atcgcatttt tctgctcagc aacgtgcgtt gttcagaaac
    15301 ttagttatat tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt
    15361 tactgaggct atttgcgcgt ttttaacatt accgctcatt aacgctttgc tggaataggt
    15421 tttgcccgga acagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct
    15481 gacgtcacat gccggttcca caatttggcc ataaaaatgg atcacccccc cttgcgcggt
    15541 gcttcctgcg atggcaataa aaggaaaaag caaactgccg agcataagag agtttaaaaa
    15601 aggtctgaac atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc
    15661 atactttcag gttggctgat tcgcagcaga ccattctctc cagattcatc ttatgctcga
    15721 tatttcaaca aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga
    15781 ctgtccatca gaaacagtaa caactatttt cacccgatct tccggggaaa tatttaaact
    15841 caacttcgtc aaagcaatag gttgattagc aggcaatgag agggtctttt cttgcttcgt
    15901 ctgactttgc cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga
    15961 ttgagtaaga gtgacttcag gaataatggt atacacatcc ccttgctggg tcgtattaaa
    16021 ggttatctgg ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa
    16081 aaaaacaggg cttgcgccct gtttctgtaa tacaaatgat gtattagtac tgatgagcgg
    16141 tcgcgttgtt accaaagcca acctgagtca cgttgacgga ggagttagat gcagtctggt
    16201 caactgcagc accgttgcca ccaccgaact gtttaacagt catttcagaa tttttgccgt
    16261 tccactgatc aagagtggcg ctgttaccga agccacgttg ggtcagatcg attgagctgt
    16321 catctgagcc ctgacctaca tctgcaccat taccaccgcc atgctgggta atagtcaagt
    16381 cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt accgccaccg tactggtaaa
    16441 tgttcagctc tgaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc
    16501 cgtactgagg aacaacacct gccagagcgc taccggagaa tacgattgct gcaattgctg
    16561 ctacttttaa aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat
    16621 taacgttgtg tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt
    16681 tgagtaccat actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca
    16741 gtattaccat aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata
    16801 tatgcaaggt tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc
    16861 gcaacaaccg ccaaaagttt tgagcctccc tgccgtaact gagcactatt attcgtccca
    16921 gcttgaccaa ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag
    16981 ttatattctg aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt
    17041 attgttaaca tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac
    17101 atttaagaaa ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct
    17161 gcgttacgat ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttgtat
    17221 ttttcttttt ttgttcatac caaagtatta aaacccacgt aaatacggtg ttatctacgc
    17281 aaaaaatatt ttgttttctt ttaaatctcc gttttccgct atcaaaaatc accagacagt
    17341 catttttctt gcccgtcgct gattgctgcg atatgtcttg cgcacaagcc gtgacaaacc
    17401 tcgtcttttt gcagtaaaaa attgtccacg gaggtgcgga gaaaaaac
//
LOCUS       DAZGCS010000100        17391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGCS010000100 DAZGCS010000000
VERSION     DAZGCS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.100"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..1326)
                     /gene="gltA"
                     /locus_tag="ACHK01_003725"
     CDS             complement(43..1326)
                     /gene="gltA"
                     /locus_tag="ACHK01_003725"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO8098611.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1532..1639)
                     /locus_tag="ACHK01_003726"
     CDS             complement(1532..1639)
                     /locus_tag="ACHK01_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098612.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2020..2424
                     /gene="sdhC"
                     /locus_tag="ACHK01_003727"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2020..2424
                     /gene="sdhC"
                     /locus_tag="ACHK01_003727"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8098613.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2418..2765
                     /gene="sdhD"
                     /locus_tag="ACHK01_003728"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2418..2765
                     /gene="sdhD"
                     /locus_tag="ACHK01_003728"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO8098614.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2765..4531
                     /gene="sdhA"
                     /locus_tag="ACHK01_003729"
     CDS             2765..4531
                     /gene="sdhA"
                     /locus_tag="ACHK01_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO8098615.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4547..5263
                     /gene="sdhB"
                     /locus_tag="ACHK01_003730"
     CDS             4547..5263
                     /gene="sdhB"
                     /locus_tag="ACHK01_003730"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO8098616.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5464..8265
                     /gene="sucA"
                     /locus_tag="ACHK01_003731"
     CDS             5464..8265
                     /gene="sucA"
                     /locus_tag="ACHK01_003731"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO8098617.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8280..9497
                     /gene="odhB"
                     /locus_tag="ACHK01_003732"
     CDS             8280..9497
                     /gene="odhB"
                     /locus_tag="ACHK01_003732"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO8098618.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQIIGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9591..10757
                     /gene="sucC"
                     /locus_tag="ACHK01_003733"
     CDS             9591..10757
                     /gene="sucC"
                     /locus_tag="ACHK01_003733"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO8098619.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10757..11626
                     /gene="sucD"
                     /locus_tag="ACHK01_003734"
     CDS             10757..11626
                     /gene="sucD"
                     /locus_tag="ACHK01_003734"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO8098620.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="ACHK01_003735"
     CDS             complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="ACHK01_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO8098621.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12621..14536
                     /gene="mngA"
                     /locus_tag="ACHK01_003736"
                     /pseudo
     CDS             12621..14536
                     /gene="mngA"
                     /locus_tag="ACHK01_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            14554..17187
                     /gene="mngB"
                     /locus_tag="ACHK01_003737"
     CDS             14554..17187
                     /gene="mngB"
                     /locus_tag="ACHK01_003737"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO8098622.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17212..>17391
                     /locus_tag="ACHK01_003738"
     CDS             17212..>17391
                     /locus_tag="ACHK01_003738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098623.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICG"
BASE COUNT         4203 a         4337 c         4734 g         4117 t
ORIGIN      
        1 ttgaacggcg ggttaaaata tttacaactt agcaatcaac cattaacgct tgatatcgct
       61 tttaaagtcg cgtttttcat atcctgtata cagctgacgc ggacgggcaa tcttcatacc
      121 gtcactgtgc atttcgctcc agtgggcgat ccagccaacg gtacgtgcca ttgcgaaaat
      181 gacggtgaac atggaagacg gaatacccat cgctttcagg atgataccag agtagaaatc
      241 gacgttcggg tacagtttct tctcgataaa gtacgggtcg ttcagcgcga tgttttccag
      301 ctccatagcc acttccagca ggtcatcttt ggtgcccagc tctttcagaa cttcatggca
      361 ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg tacacgcggt gaccgaagcc
      421 catcaggcgg aaagaatcat ttttatcttt cgcacgacga acaaattccg gaatgtgttt
      481 aacggagcta atttcttcca gcattttcag cgccgcttcg ttagcaccac cgtgcgcagg
      541 tccccacagt gaagcaatac ctgctgcgat acaggcaaac gggttcgcac ccgaagagcc
      601 agcggtacgc acggtggagg tagaggcgtt ctgttcatgg tcagcgtgca ggatcagaat
      661 acggtccata gcacgttcca gaatcggatt aacttcatac ggttcgcacg gcgtggagaa
      721 catcatattc aggaagttac cggcatagga gagatcgttg cgcggataaa caaatggctg
      781 accaatggaa tacttgtaac acatcgcggc catggtcggc attttcgaca gcaggcggaa
      841 cgcggcaatt tcacgatgac gaggattgtt aacatccagc gagtcgtgat agaacgccgc
      901 cagcgcgccg gtaataccac acatgactgc cattggatgt gagtcgcgac ggaaagcgtg
      961 gaacagacgg gtaatctgct cgtggatcat ggtatgacgg gtcaccgtag ttttaaattc
     1021 gtcatactgt tcctgagtcg gtttttcacc attcagcagg atgtaacaaa cttccaggta
     1081 gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg tgcagcaaaa taccttcatc
     1141 accatcaata aaagtaattt tagattcgca ggatgcggtt gaagtgaagc ctgggtcaaa
     1201 ggtgaacaca ccttttgaac cgagagtacg gatatcaata acatcttgac ccagcgtgcc
     1261 tttcagcaca tccagttcaa cagctgtgtc cccgttgagg gtgagttttg cttttgtatc
     1321 agccatttaa ggtctcctta gcgccttatt acgtaagact gccggaactt aaatttgcct
     1381 tcgcacatca acctggcttt acccgtttgt tatttggctc gccgctctgt gaaagagggg
     1441 aaaacctggg tacagagctc tgggcgcttg caggtaaagg atccattgat gacgaataaa
     1501 tggcgaatca agtacttagc aatccgaatt attaaacttg tctaccacta ataactgtcc
     1561 cgaatgaatt ggtcaatact tccacactgt tacataagtt aatcttaggt gaaataccga
     1621 cttcataact tttacgcatt atatgctttt cctggtaatg tttgtaacaa ctttgttgaa
     1681 tgattgtcaa attagatgat taaaaattaa ataaatgttg ttatcgtgac ctggatcact
     1741 gttcaggata aaacccgaca aactatatgt aggttaattg taatgatttt gtgaacggcc
     1801 tatactgccg ccaggtctcc ggaacaccct gcaatcccga gccacccagc gttgtaacgt
     1861 gtcgttttcg catctggaag cagtgttttg catgacgcgc agttatagaa aggacgctgt
     1921 ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac agaaagttaa
     1981 cctctgtgcc cgtagtcccc agggaataat aagaacagca tgtgggcgtt attcatgata
     2041 agaaatgtga aaaaacaaag acctgttaat ctggacctac agaccatccg gttccccatc
     2101 acggcgatag cgtccattct ccatcgcgtt tccggtgtga tcacctttat tgcagtgggt
     2161 atcctgctgt ggcttctggg taccagcctc tcttcccctg aaggtttcga gcaagcttcc
     2221 gcgattatgg gcagcttctt cgtcaaattt atcatgtggg gcatccttac cgctctggcg
     2281 tatcacgtcg tcgtaggtat tcgccacatg atgatggatt ttggctatct ggaagaaaca
     2341 ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt gctttcactt
     2401 ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat ggcgtacatg
     2461 atttcatcct cgttcgcgct accgctatcg tcctgacgct ctacatcatt tatatggtcg
     2521 gttttttcgc taccagtggc gagctgacat atgaagtctg gatcggtttc ttcgcctctg
     2581 cgttcaccaa agtgttcacc ctgctggcgc tgttttctat cttgatccat gcctggatcg
     2641 gcatgtggca ggtgttgacc gactacgtta aaccgctggc cttgcgcctg atgctgcaac
     2701 tggtgattgt cgttgctctg gtggtttacg tgatttatgg attcgttgtg gtgtggggtg
     2761 tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg attggtgccg gtggcgcagg
     2821 tatgcgcgcg gcgctgcaaa tttcccagag cggtcaaacc tgtgcgctgc tctctaaagt
     2881 cttcccgacc cgttcccata ccgtttctgc gcaaggcggc attaccgttg cgctgggtaa
     2941 tacccatgaa gataactggg aatggcatat gtacgacacc gtaaaaggtt cggactatat
     3001 cggtgaccag gacgcgattg aatatatgtg taaaaccggg ccggaagcga ttctggaact
     3061 cgaacacatg ggcctgccgt tctcgcgtct tgatgatggt cgtatctatc aacgtccgtt
     3121 tggcggtcag tcgaaaaact tcggcggcga gcaggcggca cgcactgcgg cagcagctga
     3181 ccgtaccggt cacgcactgt tgcataccct ttatcagcag aacctgaaaa accacaccac
     3241 tattttctcc gagtggtatg cgctggatct ggtgaaaaac caggatggcg cggtggtggg
     3301 ttgtactgca ctgtgcatcg aaactggtga agtggtttat ttcaaagccc gcgctaccgt
     3361 gctggcgact ggcggggcag ggcgtattta tcagtccacc actaacgccc acattaacac
     3421 tggcgacggc gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata tggaaatgtg
     3481 gcagttccac ccgaccggta ttgccggtgc gggcgtactg gtcaccgaag gttgccgtgg
     3541 tgaaggcggt tatctgctga acaaacatgg cgaacgcttt atggagcgtt atgcgccgaa
     3601 cgccaaagac ctggcgggcc gtgacgtggt ggcgcgttcc atcatgattg aaatccgtga
     3661 aggtcgcggc tgtgatggtc cgtgggggcc acatgcgaaa ctgaaactcg accacctggg
     3721 taaagaagtt ctcgaatccc gtctgccggg tattctggaa ctctcccgta ccttcgctca
     3781 cgttgatccg gtgaaagagc cgattccggt tatcccaacc tgtcactaca tgatgggtgg
     3841 tattccgacc aaagtgaccg gtcaggcgct gacggtgaac gagaaaggcg aagatgtggt
     3901 tgttccgggg ctgtttgccg ttggtgaaat cgcttgtgta tcggtacacg gcgctaaccg
     3961 tctgggcggc aactcgctgc tggacctggt ggtctttggt cgcgcagcgg gtctgcatct
     4021 gcaagagtct atcgccgagc agggcgcatt gcgcgatgcc agcgagtctg atgttgaagc
     4081 atctctggat cgtctgaacc gctggaacaa taatcgtaac ggtgaagatc cggtggcgat
     4141 tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg gtcttccgtg aaggtgatgc
     4201 gatggcgaaa gggcttgagc agttgaaagt tatccgcgag cgtctgaaaa atgcccgtct
     4261 ggatgacact tccagcgagt tcaacaccca gcgcgttgag tgcctggaac tggataacct
     4321 gatggaaacg gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa gccgtggcgc
     4381 gcatagccgc ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc actccctgta
     4441 tctgccagag tcggaatcca tgacgcgccg aagcgtcaac atggaaccga aactgcgccc
     4501 ggcattcccg ccgaagattc gtacttacta atgcggagac aggaaaatga gactcgagtt
     4561 ttcaatttat cgctataacc cggatgttga tgatgctccg cgtatgcagg attacaccct
     4621 ggaagcggaa gaaggtcgcg acatgatgtt gctggatgcg cttatccagc tgaaagagaa
     4681 agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt ccgacggtct
     4741 gaacatgaac ggcaagaatg gtctggcctg tattaccccg atttcggcac tcaaccagcc
     4801 gggcaagaag attgtgattc gcccgctgcc aggtttaccg gtgatccgcg atttggtggt
     4861 agacatggga caattctatg cgcaatatga gaaaattaag ccttacctgt tgaataatgg
     4921 acaaaatccg ccagctcgcg agcatttaca gatgccagag cagcgcgaaa aactcgacgg
     4981 gctgtatgaa tgtattctct gcgcatgttg ttcaacctct tgtccgtctt tctggtggaa
     5041 tcccgataag tttatcggcc cggcaggctt gttagcggca tatcgtttcc tgatcgatag
     5101 ccgtgatacc gagactgaca gccgcctcga cggtttgagc gatgcattca gtgtattccg
     5161 ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag gggctgaacc cgacgcgcgc
     5221 catcggccat atcaagtcga tgttgttgca acgtaatgcg taattaaatg ttgatgccgg
     5281 ggaaatcccc cggctattca ggaaatcttt aaaaactgcc cctgacacta agacagtttt
     5341 taaaggttcc ttcgcgagcc actacgtaga caagagctcg caagtgaacc ccggcacgca
     5401 cataactgtg cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc
     5461 acgatgcaga acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac
     5521 cagagctgga tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct
     5581 aactggcgtt cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac
     5641 tctcaaacgc gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg
     5701 atctccgacc ctgacaccaa tgtaaagcag gttaaagtcc tgcagctcat taacgcatac
     5761 cgcttccgtg gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa
     5821 gtggccgatc tggatccgtc tttccacgat ctgaccgaag cagacttcca ggagaccttc
     5881 aacgtcggtt catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc
     5941 ctcaagcaaa cctactgcgg cccgattggt gccgagtata tgcacattac cagcaccgaa
     6001 gaaaaacgct ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag
     6061 aaaaaacgct tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggcgca
     6121 aaattccctg gcgcaaaacg cttctcgctg gaaggcggtg acgcgttaat cccgatgctt
     6181 aaagagatga tccgccacgc tggcaacagc ggcacccggg aagtggttct cgggatggcg
     6241 caccgtggtc gtctgaacgt gttggtgaac gtgctgggta aaaaaccgca agacttgttc
     6301 gacgagtttg ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg
     6361 ggcttctcgt ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac
     6421 ccgtctcacc ttgagattgt aagcccggtc gttatcggct ctgttcgtgc ccgtctggac
     6481 agacttgatg agccgagcag caacaaagtg ctgccaatta ccatccacgg tgacgccgca
     6541 gtgaccggac agggcgtggt tcaggaaacc ctgaacatgt cgaaagcgcg tggttatgaa
     6601 gttggcggta cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg
     6661 ctggatgccc gttctacgcc gtactgtact gatatcggca agatggttca ggcaccgatt
     6721 ttccacgtta acgcggacga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat
     6781 ttccgtaaca cctttaaacg tgatgtcttc atcgacctgg tatgctaccg ccgtcacggc
     6841 cacaacgaag ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa
     6901 catccgacac cgcgcaaaat ctacgctgac aagctggagc aggaaaaagt ggcgacgctg
     6961 gaagatgcca ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgtg
     7021 gtagcagagt ggcgtccgat gaacatgcac tctttcacct ggtcgccgta cctcaaccac
     7081 gaatgggacg aagagtaccc gaacaaagtt gagatgaagc gcctgcagga actggctaaa
     7141 cgcatcagca cggtgccgga agcggttgaa atgcagtctc gcgttgccaa gatttatggc
     7201 gatcgccagg cgatggctgc cggtgagaaa ctgttcgact ggggcggcgc ggaaaacctc
     7261 gcttacgcca cgctggttga cgaaggcatt ccggttcgcc tgtcgggtga agactccggt
     7321 cgcggtacct tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac
     7381 acgccgctgc aacacatcca taacggccag ggcgcgttcc gtgtttggga ctccgtactg
     7441 tctgaagaag cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcaccctg
     7501 accatctggg aagcgcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag
     7561 ttcatctcct ctggcgagca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg
     7621 cacggttacg aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa
     7681 ctttgtgctg agcaaaacat gcaggtttgc gtaccgtcta ccccggcaca ggtttaccac
     7741 atgctgcgtc gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa
     7801 tccctgctgc gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc
     7861 ctgccagcca tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtaatg
     7921 tgttctggta aggtttatta cgacctgctg gaacagcgtc gtaagaacaa tcaacacgat
     7981 gtcgccattg tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg
     8041 ttgcagcagt ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag
     8101 ggcgcatggt actgcagcca gcatcatttc cgtgaagtga ttccgtttgg ggcttctctg
     8161 cgttatgcag gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag
     8221 aaacagcaac aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacacaa
     8281 tgagtagcgt agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg
     8341 caacctggca taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg
     8401 aaactgacaa agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc
     8461 tggaagatga aggtacaacg gtaacgtctc gtcagatcat tggtcgcctg cgtgaaggca
     8521 acagcgccgg taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac
     8581 gccagcaggc gtctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc
     8641 tgctggctga acacaatctc gacgccagcg ccattaaagg caccggtgtg ggtggtcgtc
     8701 tgactcgtga agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgctccgg
     8761 cagcggctga tccggcggcg caaccggctc tggctgcacg tagtgaaaaa cgtgtcccga
     8821 tgactcgcct gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca
     8881 tgctgaccac gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg
     8941 gtgaagcgtt tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag
     9001 cggtggttga agccctgaaa cgttacccgg aagtgaacgc gtctatcgac ggcgatgacg
     9061 tggtttacca caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga
     9121 caccggtact gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag
     9181 agctggcagt caaaggccgt gacggcaagc tgaccgttga agatctgacc ggtggtaact
     9241 tcaccatcac caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc
     9301 cgcagagcgc aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc
     9361 aggttgagat cctgccgatg atgtacctgg cgctgtccta cgatcaccgt ctgatcgatg
     9421 gtcgcgaatc cgtgggcttc ctggtaacga tcaaagagtt gctggaagat ccgacgcgtc
     9481 tgctgctgga cgtgtagtag tttaagcttc acctgcactg tagaccgacg tggatagggt
     9541 gtcggtctac ggtttaaaag ataacgatta ctgaaggatg gacagaacac atgaacttac
     9601 atgaatatca ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg
     9661 cctgtactac tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg
     9721 tagtgaaatg tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa
     9781 acagcaaaga agacatccgt gcttttgcag aaaactggct cggcaagcgt ctggtaacgt
     9841 atcaaacaga tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata
     9901 tcgctaaaga gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgc gtggtcttta
     9961 tggcctccac cgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga
    10021 tccataaagt tgcgcttgat ccgctgacag gcccgatgcc gtatcaggga cgcgagctgg
    10081 cgttcaaact gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc
    10141 tggcgaccat tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca
    10201 ccaaacaggg cgatctgatt tgcctcgacg gcaaactggg cgctgacggc aacgcactgt
    10261 tccgccagcc tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac
    10321 aggctgcaca gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta
    10381 acggcgcagg tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg
    10441 ctaacttcct tgacgttggc ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa
    10501 tcatcctctc tgacgacaaa gtgaaagccg ttctggttaa catcttcggc ggtatcgttc
    10561 gttgcgacct gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac
    10621 cggtcgtggt acgtctggaa ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca
    10681 gcggcctgaa tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg
    10741 cagtggaggg gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg
    10801 ctttactggt agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat
    10861 ggttggcggc gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa
    10921 caccgtgcgt gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc
    10981 accgttctgc aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac
    11041 catcactgaa ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc
    11101 cggcgttcgt atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat
    11161 cggtatccag ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg
    11221 tacactgacc tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg
    11281 tgttggtatc ggcggtgacc cgatccctgg ctctaacttt atcgacattc tcgaaatgtt
    11341 cgaaaaagat ccgcagaccg aagcgatcgt aatgatcggt gagatcggcg gtagcgctga
    11401 agaagaagcg gcagcgtaca tcaaagagca tgttaccaag ccagttgtgg gttacatcgc
    11461 tggtgtgact gcgccgaaag gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg
    11521 gaaagggact gcggatgaga aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg
    11581 cagcctggcg gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag
    11641 aaatagccct cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt
    11701 tggccatcgt atgatggcct tttttgtgct tatcgcgatg attttcgttg cgctatcagg
    11761 gtaaatttat agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca
    11821 actaaatatc cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga
    11881 cggctcatct cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc
    11941 attttcttca cttcttcaat aaagtgatat tttgaatttt ccatgacctg ccaggtgaga
    12001 tccgggaaca acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga
    12061 tagcgcacgc gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga
    12121 agaaaatcgt cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga
    12181 cggtccgaca gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct
    12241 tctttgacgt aagtcccgct gccctgaatg ctttcgagga tctgttgctc gactaactgg
    12301 cgcaaagcct gacgcaccgt aacccggctg acgccaaact ctgtttgtaa tgccgattcg
    12361 gtaggtaacg catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg
    12421 gcaatctgcc ggtataaggg cttgtgtccc atttttagta tctcattaat acgaatttag
    12481 ccattatgcc cgataaattc atcctgtaaa taatacaaat acaatacaaa taatttcaat
    12541 caagtgaaat tgatcacata atggtattgt tttatcgggc acactggcgc gactataaaa
    12601 acgatcaagt gaggatcatg atgaatctga cgactctgac ccaccgcgat gcgttgtgtc
    12661 tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa cgtcttgctg
    12721 ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc cgtgaaagcc
    12781 ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa
    12841 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg
    12901 gcccggaagc ggttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta
    12961 cgcatatgca actgctgaca gcgctgacca cgcgcctggc ggatgatgag attcgggcgc
    13021 gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac aagggaggca
    13081 cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaacg gcctgtccgg
    13141 cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga cgcaaactcg
    13201 gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt ttaacggcgg
    13261 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg
    13321 agcgttttaa tggcatcccg cgctttcagt gcctgttgcc gagccgattc gccatgcaga
    13381 agcgttgatc caacaagcgc ttaccctcaa gcgtagcgat gagacgcgta ccgtacagca
    13441 agatacgcaa ccggtgaaaa gtgtcaaaac ggagctgaaa caggcactgt tgagcggaat
    13501 ctcttttgcc gtaccgttga ttgtcgcggg gggcacggtg ctggcggtcg cggtattact
    13561 gtcgcaaatc ttcgggctac aagatctgtt taatgaagaa aactcctggc tgtggatgta
    13621 ccgcaagctg ggcggcgggc tgctcggaat tttgatggta ccggtgctcg cggcctatac
    13681 cgcctattct ctggcagata aaccggcgtt agcgccaggc tttgcggctg gacttgccgc
    13741 caacatgatc ggctccgggt ttctcggcgc ggtcgttggc ggattgatag ccggttactt
    13801 gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa ttcaatggat tcctgacttt
    13861 ttatctctac ccggtgctcg gtacgttggg agcgggcagt ctgatgctgt ttgtggtggg
    13921 agaacctgtc gcctggatca ataactcgct taccgcctgg ctgaacggtc tgtcaggaag
    13981 taacgcgctg ttgctgggtg ccattctcgg ttttatgtgt tcctttgacc ttggagggcc
    14041 agtgaacaaa gccgcttatg cattctgcct gggcgcaatg gcgaacggcg tttacggccc
    14101 gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt accgtaaccg cttccacgat
    14161 gctcgcaccg cgcctgttta aagagtttga aattgagacc gggaaatcca cctggctgtt
    14221 agggctggca ggtattaccg aaggggcgat cccgatggcg attgaagatc cgctgcgggt
    14281 tattggttcg tttgtgctgg gctctatggt aacgggcgct attgtcggtg cgatgaatat
    14341 cggcctttcg acacccggtg ccggcatttt ctcgctcttt ttacttcatg ataatggcgc
    14401 gggcggtgtt atggcggcaa ttggctggtt tggtgcggca ttggtggggg ctgcaatctc
    14461 gactgcaatt ctcctgatct ggcggcgtca cgcggttaag catggcaact atctgactga
    14521 tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag cggtatctcg cgttcacatc
    14581 accccgcata tgcattggga tcgggagtgg tatttcacca ccgaagagtc acgcattctg
    14641 ctggtcaata atatggaaga gatcctgtgc cgactggaac aggacaacga atacaaatat
    14701 tacgtactcg acgggcaaac ggcgatcctc gaagattatt tcgcggtgaa accggaaaac
    14761 aaagaccgcg tgaagaaaca ggtagaagcc ggcaagttga ttatcggccc ctggtatacc
    14821 cagaccgata ccacgattgt ttctgcggaa tccatcgtcc gtaatctgat gtacggaatg
    14881 cgtgactgcc tcgcgtttgg cgagccgatg aaaataggtt atttaccaga ttcctttggc
    14941 atgtccgggc aactgccgca tatctacaat ggatttggca ttacccgcac catgttctgg
    15001 cgcggatgtt cggagcgcca cggtactgat aaaaccgagt ttttgtggca aagcagtgac
    15061 ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg ccatcggtaa gtacttacct
    15121 gccgacgaaa acggattacg taaacgcctc gacagttatt ttgacgtgct ggaaaaagcg
    15181 tctgtaacca aagagatttt gctgccgaat gggcatgacc agatgccatt gcagcaaaat
    15241 atcttcgaag tgatggataa gctacgtgag atctaccctc aacgtaagtt tgtgatgagc
    15301 cgctttgaag aggtatttga gaagatcgaa gcgcagcgtg aaagtctggc caccctgaaa
    15361 ggtgaattta tcgatggcaa atatatgcgc gtacatcgca ccatcggttc cacgcgcatg
    15421 gatatcaaaa ttgcccacgc acgtattgaa aataagattg ttaatctgct ggaaccgctg
    15481 gcaacgctgg cctggacgtt gggttttgaa taccaccacg gcttgctgga gaaaatgtgg
    15541 aaagagatct taaaaaatca tgcccacgac agtatcggtt gttgttgcag tgacaaagtt
    15601 catcgcgaaa ttgtcgcccg cttcgaactg gctgaagaca tggcggataa tctgctgcgt
    15661 ttctacatgc gcaaaattgc cgataacatg ccacagagtg atgccgacaa actcgtgctg
    15721 tttaacctga tgccctggcc gcgggaagaa gttattaaca ccactgtgcg cctgcgcgcc
    15781 agccagttta atctacgaga cgatcgcggt cagcctgtac cgtattttat tcgccatgcc
    15841 cgtgagatcg atccaggcct aatcgatcgg caaatagttc attacggtaa ttacgatccc
    15901 tttatggagt ttgatataca gatcaaccag attgtccctt ctatgggcta tcgcacgctt
    15961 tatatcgaag cgaatcagcc tggcaacgtg attgcagcaa aaagtgacgc tgaagggata
    16021 ctggaaaatg ctttctggca aattgcgctc aatgaggatg gaacgctaca actggtagat
    16081 aaagatagcg gtgtgcgcta tgaccgggtg ttgcaaattg aagaaagctc tgatgatggt
    16141 gatgaatatg actattcacc cgcaaaagaa gagtgggtaa tgacttctgc aacagcgaaa
    16201 ccgcaatgcg agattactca tgaggcctgg cagagcaggg ctgttatccg ctatgacatg
    16261 gcggtgccgc tcaatttgtt ggaacgaagc gtccggcaat ccacgggcag agtaggggtg
    16321 gagatggttg tcactctgag tcataacagc agacgtattg atgtggatat caaccttgat
    16381 aaccaggctg acgatcatcg ccttcgcgtc ctgatcccta catcttttaa caccgatagt
    16441 gttctggcag atacccagtt tggttcgcta acgcgccccg tgaacgacag tgcaatgaac
    16501 aactggcagc aagaaggctg gaaagaagcg ccggttccgg tatggaatat gctcaactat
    16561 gcagccttac aggaagggcg taacggcatg gctgtcttta gcgaagggct acgtgaattt
    16621 gaagtcatcg gtgaagagaa gaaaaccttt gccattacgt tgctgcgtgg cgtgggctta
    16681 ctgggcaaag aagatctgtt tttaaggcct gggcggcctt cgggaattaa aatgccagtc
    16741 ccggactcac aactacgtgg tctgctttct tgtcgcctaa gtttattgag ttataccggt
    16801 acgccaaccg ccgctggtgt agctcagcag gcgcgagcat ggctgactcc agtacagtgt
    16861 tacaacaaaa tcccatggga tgcgatgaag ctcaacaaag ccggattcaa cgtgccggaa
    16921 agttatagtt tgttgaaaat gcccccagtg ggatgcctga taagcgcact taagaaagct
    16981 gaagaccgac aagaagtgat tttacggctg tttaatccgg ctgaatcagc aacctgtgat
    17041 gcgactgttg ctttcagtcg cgaggtgatt tcttgctcag aaacgatgat ggatgaacac
    17101 attaccaccg aggaaaatca aggttcaaat ctatcggggc cttttttacc cggccagtca
    17161 cggacgttca gttaccggct tgcctgaata gcaatcaaac cgaagccaca tatgcgcggc
    17221 cagattgttg acaaagggcg ctttgttcat gccggatacg gcatgaacgc tttattcggt
    17281 ctacaaaagc aggcaaattc aatatattgc agagatgatg tagacactgg caagcgtagc
    17341 gcatcaggta attttgcgtt tatcttcact ctcaagccac gtatatgtgg c
//
LOCUS       DAZGCS010000101        17333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGCS010000101 DAZGCS010000000
VERSION     DAZGCS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.101"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            355..441
                     /gene="leuL"
                     /locus_tag="ACHK01_003739"
     CDS             355..441
                     /gene="leuL"
                     /locus_tag="ACHK01_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO8098624.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            534..2105
                     /gene="leuA"
                     /locus_tag="ACHK01_003740"
     CDS             534..2105
                     /gene="leuA"
                     /locus_tag="ACHK01_003740"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO8098625.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2105..3196
                     /gene="leuB"
                     /locus_tag="ACHK01_003741"
     CDS             2105..3196
                     /gene="leuB"
                     /locus_tag="ACHK01_003741"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO8098626.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3199..4599
                     /gene="leuC"
                     /locus_tag="ACHK01_003742"
     CDS             3199..4599
                     /gene="leuC"
                     /locus_tag="ACHK01_003742"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO8098627.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4610..5215
                     /gene="leuD"
                     /locus_tag="ACHK01_003743"
     CDS             4610..5215
                     /gene="leuD"
                     /locus_tag="ACHK01_003743"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO8098628.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5264..6442)
                     /gene="setA"
                     /locus_tag="ACHK01_003744"
     CDS             complement(5264..6442)
                     /gene="setA"
                     /locus_tag="ACHK01_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO8098629.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6544..6675)
                     /gene="sgrT"
                     /locus_tag="ACHK01_003745"
     CDS             complement(6544..6675)
                     /gene="sgrT"
                     /locus_tag="ACHK01_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO8098630.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6764..8419
                     /gene="sgrR"
                     /locus_tag="ACHK01_003746"
     CDS             6764..8419
                     /gene="sgrR"
                     /locus_tag="ACHK01_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO8098631.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8453..8552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8583..9566
                     /gene="thiB"
                     /locus_tag="ACHK01_003747"
     CDS             8583..9566
                     /gene="thiB"
                     /locus_tag="ACHK01_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO8098632.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9542..11152
                     /gene="thiP"
                     /locus_tag="ACHK01_003748"
     CDS             9542..11152
                     /gene="thiP"
                     /locus_tag="ACHK01_003748"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO8098633.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11136..11834
                     /gene="thiQ"
                     /locus_tag="ACHK01_003749"
     CDS             11136..11834
                     /gene="thiQ"
                     /locus_tag="ACHK01_003749"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO8098634.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11948..12712)
                     /gene="yabI"
                     /locus_tag="ACHK01_003750"
     CDS             complement(11948..12712)
                     /gene="yabI"
                     /locus_tag="ACHK01_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO8098635.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQNGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12798..13676)
                     /gene="araC"
                     /locus_tag="ACHK01_003751"
     CDS             complement(12798..13676)
                     /gene="araC"
                     /locus_tag="ACHK01_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO8098636.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14015..15715
                     /gene="araB"
                     /locus_tag="ACHK01_003752"
     CDS             14015..15715
                     /gene="araB"
                     /locus_tag="ACHK01_003752"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO8098637.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPLLNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15726..17228
                     /gene="araA"
                     /locus_tag="ACHK01_003753"
     CDS             15726..17228
                     /gene="araA"
                     /locus_tag="ACHK01_003753"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO8098638.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         4149 a         4756 c         4600 g         3828 t
ORIGIN      
        1 ttaattctta acattaattg atcaataata ttcaccaaat caatatcaaa aaaaaaatcg
       61 caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc
      121 cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag
      181 cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgcagca caattagcta
      241 attttacggg tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc
      301 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
      361 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
      421 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
      481 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
      541 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
      601 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
      661 tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa accatcgccc
      721 gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg
      781 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgctacct
      841 ccccaatgca catcgccacc aagctgcgca gcaccctgga cgaggtgatc gaacgcgcta
      901 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
      961 ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
     1021 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
     1081 tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata
     1141 cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac
     1201 gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag
     1261 aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc
     1321 accaggaaat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
     1381 caaacaaagc cattgttggc agcggcgcat tcgcacactc ctccggtatc caccaggatg
     1441 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
     1501 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggacg
     1561 agatggggta taaagaaagt gaatataatt tagacaattt gtacgacgcc ttcctgaagc
     1621 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
     1681 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
     1741 acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg
     1801 ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata
     1861 acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc
     1921 aggtggatat tgtcgccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
     1981 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg
     2041 cagaagtcga aaaagagttg caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg
     2101 tgtgatgtcg aagaattacc atattgccgt attgccgggg gacggtattg gtccggaagt
     2161 gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac
     2221 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc
     2281 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggtcc
     2341 gaaatgggaa catttaccac cagaccagca accagaacgc ggcgcgctgt tgcctttgcg
     2401 taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc
     2461 attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatcctgt gcgtgcgcga
     2521 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga
     2581 aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc
     2641 gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag ctaacgtgct
     2701 gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat acccggatgt
     2761 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
     2821 gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
     2881 gatcactggc tcgatgggga tgttgccttc cgccagcctg aacgagcaag gttttggtct
     2941 gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc aaaaacatcg ccaacccgat
     3001 tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc ctggatgccg atgatgcggc
     3061 ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa ggcattcgca ccggggattt
     3121 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
     3181 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta
     3241 gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa
     3301 gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
     3361 ggcaaaacct tcgccactat ggatcacaac gtctctaccc agacgaaaga cattaatgcc
     3421 tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc
     3481 gtcgaactgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
     3541 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
     3601 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
     3661 acgcaaaccc tgaaacaggg ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc
     3721 gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac cggtagcgca
     3781 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa
     3841 ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg caaaagccgg tctggttgca
     3901 ccggacgaaa ccacctttaa ctatgtcaaa ggccgtttgc atgcgccgaa aggcaaagat
     3961 ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
     4021 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatcca
     4081 ggccaggtga tttccgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
     4141 gaacgcgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg cattccgctg
     4201 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg cattgaagat
     4261 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
     4321 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaatc
     4381 tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac
     4441 aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc
     4501 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct
     4561 gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa
     4621 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
     4681 aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt
     4741 taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
     4801 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
     4861 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
     4921 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
     4981 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
     5041 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
     5101 cgatgccttc cgccgccact gcatgatgaa cggtctggac agtattgggc ttaccttgca
     5161 gcacgacgac gccattgccg cttatgaagc aaaacaacct gcgtttatga attaatcccc
     5221 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct
     5281 ttgcggttaa aaataatgcg acaacagaaa taaccgcaat tacccagtag acagcaaagt
     5341 gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc ccggtagaaa
     5401 tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
     5461 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
     5521 tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact cctgccgcca
     5581 ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
     5641 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
     5701 atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg ttgcaggtcc
     5761 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag ccaccttgca
     5821 ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc ataaatgcaa
     5881 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc gtaaagccgt
     5941 aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga gaaagctgcg
     6001 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca tattcccgcg
     6061 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
     6121 tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg atagccatca
     6181 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc
     6241 agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag
     6301 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
     6361 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
     6421 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
     6481 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
     6541 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt tcttcaagca tggttaatcg
     6601 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
     6661 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
     6721 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
     6781 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
     6841 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
     6901 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
     6961 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
     7021 gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat
     7081 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
     7141 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
     7201 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
     7261 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
     7321 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
     7381 acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc cgacgccctg
     7441 gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc tgggacaagt
     7501 tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca gccatcccat
     7561 cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa ttcaggcatt
     7621 cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg ttctgccgga
     7681 aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg aggaaaaaga
     7741 gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgacagcc gcacccatcg
     7801 cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
     7861 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
     7921 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa gcctcatcct
     7981 gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
     8041 ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg
     8101 agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc tggatttttc
     8161 gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca
     8221 agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact
     8281 ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg
     8341 cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt
     8401 tgcgccgccg gatccatgat tgctggtaga taacaaaatc actacactaa cgccgttctc
     8461 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
     8521 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
     8581 aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
     8641 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga
     8701 tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg ctggaagatg
     8761 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc
     8821 tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg
     8881 gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt
     8941 ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa
     9001 gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag gatccgcgca
     9061 ccagtacgcc ggggctgggc ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc
     9121 cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag
     9181 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
     9241 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
     9301 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
     9361 aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact
     9421 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg
     9481 ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg
     9541 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
     9601 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
     9661 gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
     9721 ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc
     9781 cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
     9841 gatcctcccg gtgctagttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg
     9901 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
     9961 aggcattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca
    10021 ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg
    10081 ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc
    10141 cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg
    10201 tccgcaggca actactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc
    10261 tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt
    10321 gagtcagcga ctaagtaagg ccattgcgcc cggtaccacg ctgctgcaag gctggcgcga
    10381 tccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc tggcgctgct
    10441 gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga
    10501 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    10561 ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    10621 ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc
    10681 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct
    10741 gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    10801 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    10861 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg
    10921 tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt
    10981 ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg
    11041 ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct
    11101 gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    11161 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    11221 caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc
    11281 tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg acgccgccgt
    11341 cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac ctgacggtcg
    11401 cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg cagcaggaga
    11461 aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg ttaccgggcg
    11521 agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc
    11581 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgc
    11641 tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca
    11701 gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac gggcgcatcg
    11761 cctggcaggg taagaccaat gagttgttga gcggtaaggc gagtgcttcg gcactattgg
    11821 ggattacggg ttagtatgca ctgtcggatg cggcgtgaac gccttatccg acctacgggt
    11881 agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc atcaggcaac ccgcacaaga
    11941 ctatctccta acccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt
    12001 ggcgaattaa caccaccagc gccaccacgc cgatggcaga aattaacggc gtcagccaca
    12061 acaaacggcc gcgcgacaaa taatgactta aacgatcggt tgctttaccg ctgcgccata
    12121 accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt
    12181 taaactcgcc gttctgcatt ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag
    12241 gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgta atgaattttg
    12301 ccaccggcag atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa
    12361 agcgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga
    12421 gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa cggcttttta aaacgccagc
    12481 ccagccagaa ggagatccag tcgcccagca gacaaccgac aatccctgcc agccaggcgt
    12541 gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac
    12601 cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca
    12661 tcaatgaata cacggtggat tgggtaataa agtgttccag caatgcttgc ataatgtgcc
    12721 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
    12781 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
    12841 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg cgagaaatag
    12901 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtagtgctc
    12961 aaaagcagct tcgcctgact aatgcgttgg tcctcgcgcc agcttaagac gctaatccct
    13021 aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg ctgtgcgacg
    13081 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
    13141 acacgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
    13201 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
    13261 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
    13321 aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
    13381 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
    13441 atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga
    13501 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
    13561 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
    13621 agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
    13681 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
    13741 cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct tattaaaagc
    13801 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
    13861 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
    13921 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
    13981 tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt
    14041 tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac
    14101 cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca
    14161 gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct
    14221 tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg
    14281 ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc
    14341 cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga
    14401 agagattacc cgtttgtgcc acgcgccggg caacgttgac tactcccgct atattggcgg
    14461 tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc
    14521 cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc
    14581 cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct
    14641 gtggcacgaa agctggggcg gcctgccgcc agccagtttc tttgatgagc tggacccgct
    14701 cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt
    14761 gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc
    14821 cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact
    14881 ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg
    14941 cgagcgggca gtgaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
    15001 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggtttg gtcgcgtact
    15061 cggctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc
    15121 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
    15181 tcacctgccg gtggtgctcg actggtttaa cggccgccgc acaccgaacg ctaaccaacg
    15241 cctgaaaggg gtgattaccg atcttaacct cgctaccgac gctccgctgc tgttcggcgg
    15301 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
    15361 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
    15421 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg
    15481 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccctc
    15541 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgtgca gcgaacaggc
    15601 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
    15661 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttccgactc tataaggaca
    15721 cgataatgac gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt
    15781 atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgtcgtt aatgcgctga
    15841 atacggaagc gaaactgccc tgcaaactgg tgttgaaacc gctgggcacc acgccggatg
    15901 aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt
    15961 ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
    16021 cgttgctgca attccacacc cagtttaacg cggcgctgcc gtgggacagt atcgatatgg
    16081 actttatgaa cctgaaccag actgctcatg gcggtcgcga gttcggcttc attggcgcgc
    16141 gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca ggataaacaa gcccatgagc
    16201 gtatcggctc ctggatgcgt caggcggtct ctaaacagga tacccgtcat ctgaaagtct
    16261 gccgatttgg cgataacatg cgtgaagtgg cggtcaccga tggtgataaa gttgccgcac
    16321 agatcaagtt cggtttctcc gtcaatacct gggcggttgg cgatctggtg caggtggtga
    16381 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
    16441 tgacgcctgc aacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
    16501 ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
    16561 ttgaagattt gcacggtctg aaacagcttc caggtctggc cgtacagcgt ctgatgcagc
    16621 agggttacgg ctttgcgggc gaaggcgact ggaaaactgc cgccctgctt cgcatcatga
    16681 aggtgatgtc aaccggtctg cagggcggta cctcctttat ggaggactac acctatcact
    16741 tcgagaaagg caatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg
    16801 cagtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
    16861 ctgcccgcct gatcttcaat acccaaaccg gtccagcgat tgttgccagc ctgattgatc
    16921 tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaacc ccgcactccc
    16981 tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca accggatctg ccaactgctt
    17041 ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
    17101 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
    17161 acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc
    17221 gtcgctaagt agccacatca ggtatgtaac gcctgatgcg acgctgacgc gtcttatctg
    17281 gcctacgcgc tgtgtttttg taggccggat aagcaaagcg catccggcat tca
//
LOCUS       DAZGCS010000102        17225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGCS010000102 DAZGCS010000000
VERSION     DAZGCS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.102"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..724
                     /gene="ais"
                     /locus_tag="ACHK01_003754"
     CDS             122..724
                     /gene="ais"
                     /locus_tag="ACHK01_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO8098639.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNY"
     gene            complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHK01_003755"
     CDS             complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHK01_003755"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8098640.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEIMPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVAARKTLRLKGLL"
     gene            1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHK01_003756"
     CDS             1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHK01_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO8098641.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGSEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     GGNRDNAVADGPYVGVNASF"
     gene            2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHK01_003757"
     CDS             2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHK01_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO8098642.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3375..3836
                     /locus_tag="ACHK01_003758"
                     /pseudo
     CDS             3375..3836
                     /locus_tag="ACHK01_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3877..4779)
                     /gene="rpnE"
                     /locus_tag="ACHK01_003759"
     CDS             complement(3877..4779)
                     /gene="rpnE"
                     /locus_tag="ACHK01_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO8098643.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4973..6163)
                     /gene="glpC"
                     /locus_tag="ACHK01_003760"
     CDS             complement(4973..6163)
                     /gene="glpC"
                     /locus_tag="ACHK01_003760"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO8098644.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6160..7419)
                     /gene="glpB"
                     /locus_tag="ACHK01_003761"
     CDS             complement(6160..7419)
                     /gene="glpB"
                     /locus_tag="ACHK01_003761"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO8098645.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7409..9037)
                     /gene="glpA"
                     /locus_tag="ACHK01_003762"
     CDS             complement(7409..9037)
                     /gene="glpA"
                     /locus_tag="ACHK01_003762"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO8098646.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVAAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9310..10668
                     /gene="glpT"
                     /locus_tag="ACHK01_003763"
     CDS             9310..10668
                     /gene="glpT"
                     /locus_tag="ACHK01_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO8098647.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10673..11749
                     /gene="glpQ"
                     /locus_tag="ACHK01_003764"
     CDS             10673..11749
                     /gene="glpQ"
                     /locus_tag="ACHK01_003764"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8098648.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11791..12030)
                     /locus_tag="ACHK01_003765"
                     /pseudo
     CDS             complement(11791..12030)
                     /locus_tag="ACHK01_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12028..12132
                     /locus_tag="ACHK01_003766"
                     /pseudo
     CDS             12028..12132
                     /locus_tag="ACHK01_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12212..12862
                     /gene="inaA"
                     /locus_tag="ACHK01_003767"
     CDS             12212..12862
                     /gene="inaA"
                     /locus_tag="ACHK01_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO8098649.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12916..13170)
                     /gene="yfaE"
                     /locus_tag="ACHK01_003768"
     CDS             complement(12916..13170)
                     /gene="yfaE"
                     /locus_tag="ACHK01_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO8098650.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13170..14300)
                     /gene="nrdB"
                     /locus_tag="ACHK01_003769"
     CDS             complement(13170..14300)
                     /gene="nrdB"
                     /locus_tag="ACHK01_003769"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8098651.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14447..16732)
                     /gene="nrdA"
                     /locus_tag="ACHK01_003770"
     CDS             complement(14447..16732)
                     /gene="nrdA"
                     /locus_tag="ACHK01_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8098652.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16860..17003
                     /gene="ypaB"
                     /locus_tag="ACHK01_003771"
     CDS             16860..17003
                     /gene="ypaB"
                     /locus_tag="ACHK01_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO8098653.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4069 a         4200 c         4603 g         4353 t
ORIGIN      
        1 ttgaggcatt ttgccgacat cgtaggattt ttaatattac accaactgct aattatcgcc
       61 agaaatgtcg ctcaatttta cggtaattgt ctggttgcgc ttgtctatag gtggagttta
      121 cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc
      181 gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg
      241 tatcgacaat aaaacattgg ccagactggc acagcagcac ccggttgtcg ttttgtttcg
      301 ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac
      361 ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagcgctg acatccctga
      421 tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct ggttttccgc
      481 gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc
      541 aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca
      601 ttgcctgaca tatattgcta aaaataagcg tgatgcgaca tttaaacctg attatctgga
      661 tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaacta
      721 ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa
      781 acgtaacgtt tttcgggcgg caacatttaa atcataatgc accagatctt caggttttac
      841 ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcagtc
      901 aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg tcttggtgcg
      961 aatatcatcg ctgaaggtcc acggcatgat ttctgtcaaa agcagctgtt ctcccagttc
     1021 ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccagactcca cgccgccacc
     1081 cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata
     1141 agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcatag gggtattcct
     1201 tataaataaa aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa
     1261 tctcttttgc aatgataact gcacttatta ttatttttgt gtgtatcggt aaagtgtgtt
     1321 tattttttcg gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct
     1381 tttttcgctc attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg
     1441 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
     1501 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
     1561 acaccaatat tggtgtcggt tttgggagtg aatcgacggg cctggcttta agcggtaact
     1621 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
     1681 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
     1741 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
     1801 gtttgtttgg cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg
     1861 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
     1921 atcgctacct gaatctgtcg ggtaaaggcg gtaaccgcga caacgccgtg gctgacggcc
     1981 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
     2041 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
     2101 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
     2161 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat
     2221 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
     2281 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
     2341 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcatggt gctgcatgaa gcctggctca
     2401 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     2461 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     2521 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     2581 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     2641 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     2701 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcttca atgccgatta
     2761 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     2821 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     2881 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     2941 gcggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     3001 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     3061 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     3121 cgctagccac gccagacggc actttcgctc tgcgtgtgcg tttcagcact acacgctaca
     3181 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     3241 taaacggcca ggatatcgcc agtgagcatg gctggattga ggttattgag tccatgacct
     3301 tatctgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     3361 aaaacttgtc caaccctccc tacggtgagc gtggtgttgg ggccagtgtg gcacgggctg
     3421 cgcgctgggg acgcattgag aattacatgg cgcaagttaa cgattcactt tgtctgttgg
     3481 tgcaggtgga aagtaaaacg gcactggata acctggacga aatcctcgac gtcgaaggga
     3541 ttgatggcgt gtttattgga cctgcggatc tttctgcgtc gttgggctac ccggataacg
     3601 cagggcaccc ggaagtgcag cgaattattg aaaccagtat tcggcggatc cgtgctgcgg
     3661 gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc ctggcgtggg
     3721 gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc ctggatcaac
     3781 gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt tattgatatc
     3841 aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg ctgccagatc
     3901 ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga tcaatgccct gttccagcat
     3961 gcgcaaagca attttaatgg cttgttcatg cataccttcc tgccagccaa tttgatgccc
     4021 ttcctgccgt agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc
     4081 ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt
     4141 aaacagtgtt tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct
     4201 gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt ccagcagagc
     4261 tatacgccga tgttgcatga tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa
     4321 agcttcggtg tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttaag
     4381 tgagtacggg tagggcgatg tttcgccatg ataaaacagc aatggcacca ccagcggaac
     4441 tctgtcatag cctttatcca ggtgacgctg catggcggca gtggcatagc gcattagccg
     4501 aaaagccata ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccgtc
     4561 accgtcgctg gtttccacgg accacaaaac atccgagtag taagcgcgta aacttttttc
     4621 aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc
     4681 tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt gtgaacataa aggttttaaa
     4741 caccgcatca tgcggggagg atgttgttga ttcggtcatg gttgtccgtc acctgatatt
     4801 tatgatgacg cgacattacg tctatcccgg gcctttgaca tctgtcagtt tcagcctttg
     4861 cgagacattt tcagaggaat tttcaaagcg ttgcaacgca tacatggtgc gtggaatcgc
     4921 gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca tcaaaaggtc ggttaagcca
     4981 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag gctcgtggac atctcaatct
     5041 gccatttgca ggtttcgcag tcggtgatga ccagatccgc gccgctctct tctatctggc
     5101 ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     5161 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggattttac
     5221 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     5281 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     5341 acagccagcg ggttgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     5401 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     5461 ttccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     5521 ctttatcggt aaagccgttg gcgattagcg gtacgccgca gcatttttct ttgctgagca
     5581 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     5641 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatat tgtgcttgct
     5701 gagccgctat gctgcgatac cagcgacgga acgtgccgaa ggagtatttc ggtagcgtgc
     5761 ggcgatgatc gatttttaac gccgcatcaa gcagctgccg caccggtttc agcgaggtag
     5821 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatgactca
     5881 acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt tgcccgcgcg cgctggataa
     5941 tatcgccaat cttcacatcg gacgggcagg cgacttcaca acgtttgcag ttgatgcaat
     6001 atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg cagacgctcg ccatccggcc
     6061 cggcttgttt tggccctgga taaccgggat tcacccggct caccgggcag gcggtggtgc
     6121 agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt cattgttggc ctcctgcgcg
     6181 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     6241 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt
     6301 ttgccctgcc tgcgacgggc gtagcgtctc atcagtggtt acaccgaact gctgccacgg
     6361 ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     6421 aaggccgaga atcggctcgc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     6481 actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga atatcggcgt gattgcgggt
     6541 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     6601 ccacacgcca ccctggcgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     6661 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     6721 acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     6781 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     6841 acgggcgata ttcacagcgc gaaattcggt ggcgttattg cgcaggacat ccagttccgg
     6901 cagctctatt tctgcggttt caaccgaaag atccagttcg cgcaacgaag ccgccgcaag
     6961 atgcgcctgg aaatccatca gcccgcttat ccccactaca catatcttct tcgcgggtag
     7021 cggccagacg ggaacttctg gcgaacttaa ccaggttgca cgcaaagtgc cgagcggggt
     7081 aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc gcaccgcttt cagcgatcaa
     7141 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt tcgagaagac tgtaaggatg
     7201 ggctggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca ccggttgacc
     7261 atcaggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     7321 acgcgtgaca atggcacagc gcaggccgtg tttttgcagt tgcaggccac agagtaatcc
     7381 ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc tttctgctcc ttctccagac
     7441 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgcg tctccccagg
     7501 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     7561 gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc agcgcggcag gcgcagagtt
     7621 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     7681 cgtttaaatt ttctaccgcg tactgaactt cacccgcagt taccgcttcg cactcacata
     7741 ccaggctacg atgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     7801 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     7861 cgggttcttg tgaaccaggc agtgccagat cggcagtcgt acaggggcgc gtgttgccca
     7921 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     7981 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     8041 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     8101 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     8161 gcagcagaat atcaacctct tctgccgcca ctcgattatc gtcaatctcg ttgtaatcaa
     8221 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     8281 cggaaggttt acggcagcgg ttgatcacat gctggttaat gcggtgatcc atgatcagca
     8341 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     8401 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     8461 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     8521 catgagcggt aaggataacg gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     8581 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     8641 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     8701 cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc atcttccggc agggtgataa
     8761 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     8821 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     8881 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     8941 gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc cgttgcgccg ccgccaatga
     9001 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     9061 tttaccattt agccatagta aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa
     9121 ttcgaaagtg aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     9181 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     9241 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     9301 aggctatcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     9361 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     9421 tatgcggctt actatttggt tcgtaagaac tttgcgcttg ctatgcctta tctggttgag
     9481 cagggattct cacgcggtga tttaggtttt gccctttcgg ggatctcgat tgcttatgga
     9541 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     9601 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     9661 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     9721 tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     9781 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     9841 ctggggatgg cctggttcaa tgactggcat gcggcgctct atatgcccgc tttctgcgcc
     9901 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ttgtggtttg
     9961 ccgccgatcg aagagtacaa aaatgattac ccggacgact ataacgaaaa agcggaacag
    10021 gaactgacgg cgaagcagat cttcatgcag tacgtgttgc cgaacaaatt gctgtggtat
    10081 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
    10141 acttacctga aagaggttaa acatttcgcg ctggataaat cttcctgggc ctacttcttc
    10201 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
    10261 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
    10321 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
    10381 ggcttcctga tctacggtcc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
    10441 aaaaaagcgg caggtacggc agcgggcttt accgggctgt ttggttacct gggcggttcg
    10501 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
    10561 atggtaatga ttggcggcag cattctggcg gttatcttgt tgattgttgt gatgattggc
    10621 gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
    10681 gaagctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
    10741 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
    10801 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
    10861 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attatctcga
    10921 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
    10981 cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat
    11041 tgaaaacggt aaaaaagtac agacttatcc ggggcgtttc ccaatgggta agtccgactt
    11101 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
    11161 gaaaaatatc ggtatctatc cagaaattaa agcgccgtgg ttccatcatc aggaagggaa
    11221 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaggacga
    11281 caaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
    11341 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
    11401 aacgcagcag aaacagccgg atggaagctg ggttaattac aactacgact ggatgtttaa
    11461 gccgggtgct atgaaacagg tggcggaata tgccgacggt atcgggccgg attaccatat
    11521 gttgattgag gaaacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
    11581 tcagcagaat aaactggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
    11641 cactactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggct
    11701 gtttactgat ttccctgata aggcagtaaa attccttaat aaagagtaat gctactgcag
    11761 gatggcgttc atgcgccatc ctgtctcaca tcaaattttt ccattcacca cgagttgttc
    11821 cgtacacgtt tgtagtaaaa acagtggact gccttctacc ggcttttctt tagccaacaa
    11881 cgaaatctgt cgatagaagg ttggttcgtg aggaacggaa cacaattcac ctgcaatgct
    11941 tttcagcgtt aactctggtt gtaaggcgat gcctaatccc tgtcgaataa acttcatcgc
    12001 tgtatccgac tgattaaatt cacattttat ctactgggcg cactattagt ggtagtggct
    12061 ggtggaatct atgcgctgca aatgaagggt aaaagcggtg tcacgctagt agtggcaaaa
    12121 gaaattcatt aatacgacac gtttcattaa gattttcctc aggtaaccac ctatagtcat
    12181 tgtcgttaat aaaaatgaca ggcgataggg tatggcagtt tcagcaaagt acgacgaatt
    12241 taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg
    12301 tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa agcgcatgac
    12361 gcatcatctg tttcattccg tacgttatcc gttcggccga ccaacgattg tccgtgaggt
    12421 tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga
    12481 agcggtgaaa attgagggtg aatggcgagc gttgctggtg actgaagata tggcggggtt
    12541 catcagcatt gctgactggt atgcccagca tgcagtatcg ccttattctg acgaagtacg
    12601 gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagca ttaatcgtca
    12661 gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaatg cagaagctgg
    12721 ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga ttaatcatga
    12781 cttccgccag ctggaaaaat atctggagcc aatccccaaa gcggactggg agcaggtgaa
    12841 agcgtattac tacgccatgt aatcgttgta ggccagataa gacgcataag cgttgtatct
    12901 ggcactacaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc aacaacaggg
    12961 caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcaa cctgacctgc
    13021 aaccagacgg gtgcgacagg agccgcagta accttcgcga cactgatact caaccgccac
    13081 attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca gcagttgtgt
    13141 gccagtgatg cgcagggtaa cgcgggccat cagagttgga agttactcaa atcgtcggtg
    13201 tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc ctgcggagca
    13261 acctgcacgt tatcagacac cagccaggtg ttgatccatg ggatcgggtt ggagcgcgtc
    13321 tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta ttcaacgtac
    13381 tggcagagaa tgtctttatt cagaccaatc atcgaaccgt cgcggaacag ataatccgcc
    13441 cagtctttct cctgttgagc tgcctgaaca aacaggtcat agcactcctg cttacactct
    13501 tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc gcagcagatt cagcatatgc
    13561 tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat tttggcgttg
    13621 ccttccatca attcgcgttc tgcaaatgcg aaggaacaag caaagctgac gtagaaacga
    13681 atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt cagctcgcgc
    13741 aggctaacgg tcacagtttt accgttaacg gtgtgggtac cttcgcccag cagatgccag
    13801 tagctggtca tttcgatcag ctcatcgtaa tagctggaga tcccttccgc acgtttctgg
    13861 atctgctcgt tggtgacgat atcgtcaaac acaacagacg gatcgttaac gatattacga
    13921 atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt ttcgacccag
    13981 gtctccagtt ccgggatcga gatcagcggc agcagtgcca cgttcgggct acggccctga
    14041 atggaatcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgttca
    14101 ggcagtgcct ggtaatcgat acggtcgcga gaaacgtcaa cttcttccgg acgccagaag
    14161 aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga
    14221 gccacgttga ccggctgacc aaagaacatc ggttctttga gctgatcatt tttcgtctgt
    14281 gaaaaggtgg tatatgccat gagtgtgtcc tgttgggagt ttaatgccgg ataaggcatt
    14341 ttacgccgca tcctgtgcct gatgcgacgc tgacgcgtct tatcaggcct acaaatccga
    14401 gccgtaggcc ggataaggcg ttcacgctgc atccggcatc tcaatatcag atcttacatg
    14461 cgccgctttc gcagccatcg tcctggattg acggtaccag atcgtcctgc gcgtcttcag
    14521 cgccgtcacg ggtgttctga taatacagcg ttttgacccc gaatttgtag gcggtgagca
    14581 ggtctttcag caactgctgc atcggcactt ttcctgacgg gaaacgtgac ggatcgtagt
    14641 tggtgttagc agagatcgac tgatcgataa atttctgcat gatacctacc agttgcaggt
    14701 agccatcgtt acccggcatt tcccacagca gctcataggc gtcgtgcagg tgctcgtagt
    14761 ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt gatgctgacg taaccgcgcg
    14821 gcggttcaat accgttagtg gcgttagaga tctgtgaaga agtctctgac ggcatcaggg
    14881 cagaaagcgt ggagttacgc aggccgtgcg ttttgattga ttcacgcaat gcttcccagt
    14941 cgtaatgcag cggctcatta gcgatggtat ccagatcttt cttataggta tcgatcggca
    15001 ggatcccttt cgcgtaagtg gtttcgttaa accacgggca cgcgccttgc tcttttgcca
    15061 gctcgttaga cgctttcagc aggtaatact gaatcgcttc gaaggtttta tgcgtcaggt
    15121 tgttggcgct gccgtcggag taacgcacac cgtgcttcgc caggtagtaa gcgaagttga
    15181 tcacaccaat acccagcgta cgacgaccca tcgctccacg tttggcggcc gggatcgggt
    15241 aatcctgata atccagcagc gcgtcaagtg cacgaaccgc cagaattgcc agctcttcca
    15301 gttcatccag gctattaatt gcgcccaggt tgaaagcaga cagcgtacac agcgcgattt
    15361 caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg cagggctatc tccaggcaca
    15421 ggttagactg acgcactggc gcgatggccg gatcaaacgg gctatgggta ttgcagtggt
    15481 caacgttctg aatatagata cgaccggtag acgcacgttc ctgcatcatc agcgagaaca
    15541 gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc tttctcatat ttggtataca
    15601 ggcgttcaaa ctcttcctga tcggcgaaga atgcgtcgta cagccccggt acgtcggacg
    15661 ggctgaacag ggtgatatct tcacctttca gcaggcgggt atacatcagt ttgttgatct
    15721 gtaccccgta gtccatatgg cgcacgcggt tgccttccac accacggttg tttttcaaca
    15781 ccagcaggct ttccacttcc agatgccaca tcgggtagaa cagcgttgcc gcaccgccgc
    15841 gcacgccgcc ctgagagcag gatttcaccg ctgtctggaa atgtttgtag aacggaatac
    15901 agccggtatg gaacgcttca ccaccgcgaa tcgggctacc cagcgcacga atacgcccgg
    15961 cgttgatgcc gatcccggca cgctgggaaa cgtatttcac aatcgcgctg gaggtagcgt
    16021 tgatggaatc cagactgtca ccgcactcga tcagtacgca ggagctgaac tgacgagtcg
    16081 gggtacgcac gccggacatg attggcgtcg gcagcgaaat tttaaatgtg gaaaccgcgt
    16141 cgtaaaaacg cttcacatat tgcaggcgcg tttcacgcgg gtagttcgag aacaggcacg
    16201 cggcaaccag aatataaagg aactgggcgc tttcgtagat ttcgccggtc acgcggttct
    16261 gtaccagata tttgccttcc agctgcttaa cggcagcata agagaaggtc atatcacggt
    16321 cgtgatcgat aaacgtgtcc atctgcttga actcttcttc cgtgtagtct tccagcagat
    16381 gattatcgta tttgcccatc tcgaccattt tcaccacgtg gtcgtacagc gcaggcggct
    16441 caaactggcc gtaggctttt ttacgcaggt ggaagatcgc caggcgcgcg gcgagatact
    16501 gataatccgg cgcatcacgg gagatcaggt ctgcggccgc cttgataatg gtttcgtgga
    16561 tgtcagaggt cttgataccg tcataaaact gaatgtggga gcgcagctcg acctgggaaa
    16621 tcgaaacgtt atgcagtcct tctgccgccc aatccagaac gcgatggatt ttgtcgagat
    16681 tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag attctgattc atgtatgtcg
    16741 tacctgtttt tggaattctt tccgcaatag tttcatgcag actacctgta gtaagggaag
    16801 ctgcttagaa cactatatat agggggtatg tttgggattc actgcaagat agtgtgaaaa
    16861 tggccctctt gcaagtgcat aactttgtgg ataactcagg aaggaaaaag tggctttcgc
    16921 gcaccttagg tcagacaagg tgtccgggaa agtcaatggg aagagaaaaa tttgttaaaa
    16981 ataactgttc ggcgatttct tgaacggcag aataggttgg ataaattgat atagatggtt
    17041 tattaatctt tttgctaatg attgcaaaaa ttgatctaag cacgagtcat cacataatct
    17101 gatgtatcag ttgtggtcaa tatataaacg aatgtaagcg attgttgatg tcgaaatggt
    17161 tgttttctat tcggttattg ttaaatgcga agccattttg ttaatttacc ttatattata
    17221 agaaa
//
LOCUS       DAZGCS010000103        16875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGCS010000103 DAZGCS010000000
VERSION     DAZGCS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.103"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            116..658
                     /gene="ecpR"
                     /locus_tag="ACHK01_003772"
                     /gene_synonym="matA"
     CDS             116..658
                     /gene="ecpR"
                     /locus_tag="ACHK01_003772"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO8098654.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            733..1320
                     /gene="ecpA"
                     /locus_tag="ACHK01_003773"
                     /gene_synonym="matB"
     CDS             733..1320
                     /gene="ecpA"
                     /locus_tag="ACHK01_003773"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO8098655.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1382..2050
                     /gene="ecpB"
                     /locus_tag="ACHK01_003774"
                     /gene_synonym="yagY"
     CDS             1382..2050
                     /gene="ecpB"
                     /locus_tag="ACHK01_003774"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO8098656.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2076..4601
                     /gene="ecpC"
                     /locus_tag="ACHK01_003775"
                     /gene_synonym="yagX"
     CDS             2076..4601
                     /gene="ecpC"
                     /locus_tag="ACHK01_003775"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO8098657.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4591..6234
                     /gene="ecpD"
                     /locus_tag="ACHK01_003776"
     CDS             4591..6234
                     /gene="ecpD"
                     /locus_tag="ACHK01_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO8098658.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSQESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6203..6913
                     /gene="ecpE"
                     /locus_tag="ACHK01_003777"
     CDS             6203..6913
                     /gene="ecpE"
                     /locus_tag="ACHK01_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO8098659.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7226..7555
                     /gene="ykgJ"
                     /locus_tag="ACHK01_003778"
     CDS             7226..7555
                     /gene="ykgJ"
                     /locus_tag="ACHK01_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8098660.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7803..8415)
                     /gene="yagU"
                     /locus_tag="ACHK01_003779"
                     /pseudo
     CDS             complement(7803..8415)
                     /gene="yagU"
                     /locus_tag="ACHK01_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
     gene            8833..9522
                     /gene="paoA"
                     /locus_tag="ACHK01_003780"
     CDS             8833..9522
                     /gene="paoA"
                     /locus_tag="ACHK01_003780"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO8098661.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAEEIKS"
     gene            9519..10475
                     /gene="paoB"
                     /locus_tag="ACHK01_003781"
                     /pseudo
     CDS             9519..10475
                     /gene="paoB"
                     /locus_tag="ACHK01_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
     gene            10472..12670
                     /gene="paoC"
                     /locus_tag="ACHK01_003782"
     CDS             10472..12670
                     /gene="paoC"
                     /locus_tag="ACHK01_003782"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO8098662.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12680..13636
                     /gene="paoD"
                     /locus_tag="ACHK01_003783"
     CDS             12680..13636
                     /gene="paoD"
                     /locus_tag="ACHK01_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO8098663.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHLTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLETQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            13687..14025
                     /locus_tag="ACHK01_003784"
                     /pseudo
     CDS             13687..14025
                     /locus_tag="ACHK01_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(14152..14227)
                     /locus_tag="ACHK01_003785"
     tRNA            complement(14152..14227)
                     /locus_tag="ACHK01_003785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14192..14194),aa:Thr,seq:cgt)
     gene            complement(14342..15595)
                     /gene="proA"
                     /locus_tag="ACHK01_003786"
     CDS             complement(14342..15595)
                     /gene="proA"
                     /locus_tag="ACHK01_003786"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO8098664.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(15607..16710)
                     /gene="proB"
                     /locus_tag="ACHK01_003787"
     CDS             complement(15607..16710)
                     /gene="proB"
                     /locus_tag="ACHK01_003787"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO8098665.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         4128 a         4550 c         4461 g         3736 t
ORIGIN      
        1 atcttattta cttccgattt actaaagaaa ctgaatgtac ctgtaaaaat tacaggtttg
       61 gaaagtagtg acatggcaaa atgattacag cagggactat gaggttaaaa accatatgga
      121 atgtcaaaac cgttctgata aatacatctg gtctccccat gacgcctact tctataaagg
      181 actatctgaa ctgattgtgg atatcgacag attaatttat ctatcgctgg agaaaatcag
      241 aaaagatttc gtgtttatca atctcaatac ggattcttta actgaattta taaaccgtga
      301 taatgagtgg ttatccgcgg taaaggggaa acaggtcgta ttgattgcgg ccagaaagtc
      361 agaagcctta gcaaattatt ggtattacaa cagcaatatt aggggcgtgg tatacgctgg
      421 actgagtcgt gatattagaa aagaactggc ctatgtgatt aatggcaggt tcctgagaaa
      481 agatattaag aaagataaaa tcactgaccg agaaatggaa attatccgca tgacggctca
      541 gggaatgctg cctaaatcga ttgccagaat tgaaaattgt agtgtaaaga cagtgtatac
      601 ccatcggcgt aatgcagagg ccaagctgta ctcaaaaata tataagttgg ttcagtaaac
      661 tccaggcaag ttagttttaa aaaatgactc actgggacat cacgtcctca attcaactcg
      721 ggaagaaatg caatgaaaaa aaaggttctg gcaatagctc tggtaacggt gtttaccggc
      781 atgggtgtgg cgcaggctgc tgacgtaaca gctcaggctg tagcgacctg gtcggcaaca
      841 gccaaaaaag acaccaccag taagctggtt gtgacgccac tcggtagcct ggcgttccag
      901 tatgccgaag gcattaaagg ttttaactca cagaaaggtc tatttgacgt ggctatcgag
      961 ggtgactcaa cggctaccgc ctttaaactg acctcacgtc ttatcaccaa caccttaacc
     1021 cagttggata cctcaggttc tacactgaat gtgggcgtgg attataacgg cgcggcagtc
     1081 gaaaaaactg gcgataccgt gatgatcgat accgccaacg gcgtactggg cggcaacctt
     1141 agcccgctgg ctaacggtta caatgccagc aatcgtacca ccgcacagga tggtttcact
     1201 ttctccatca tcagcggcac caccaatggt accaccgcag taaccgatta cagcactcta
     1261 ccggaaggca tctggagcgg cgacgttagc gtacagttcg acgcgacctg gaccagttaa
     1321 tctctctgat gtaccagcag gggtagcccc cccccctgct ttattcctgg acggaattgt
     1381 tatgaaaaag caccttctgc ttctcgctct gctgttatcc ggaatatctc cggcccaggc
     1441 gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc tgagcaaaac
     1501 gatcaaaaac agtaccgaca gtggtcgcct catcaatatc cgtctcgaac ggctctcttc
     1561 accgcttgac gacgggcagg ttatcgcaat ggacaagccg gatgagttgc tactcactcc
     1621 cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc
     1681 agcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc
     1741 gcagcgcgat aatgccaacc gcagcgctgt ggccactgct tccgcccgca tcggcaccat
     1801 tctggtcgtc gcaccccgcc aggcaaacta ccactttcag tacgccaacg gctccctgac
     1861 aaatacagga aatgcgacgc tgcggatcct cgcttacggc ccctgcctga aagccgccaa
     1921 tggtaaggag tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg
     1981 cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc
     2041 cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag
     2101 gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg
     2161 ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg
     2221 cgctccagga cggcatgagc gtcccgcttt atattcatct cgccggtagc cagggtcgcc
     2281 aggacgatca gcgaatcggc agcgctttta tctggttgga tcatggacag ctacgcatcc
     2341 ggaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc
     2401 agctgatggc tctggcgaac gccccgttca atgaggccct taccatcccc ctgactgaca
     2461 acgcgcagct ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag
     2521 cgctgggcac tgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc
     2581 tcagcagtaa tctgagctat aacttcggca tctataacaa ccagttgcgt aacggcggga
     2641 gcaacacatc cagctatctg tcgctgaata acgttactgc actgcgcgaa catcatgtgg
     2701 tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa ttatataaag
     2761 cgatgtatga acgcgatttt gccggtcacc gatttgccgg tggaatgctc gacacctgga
     2821 acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct
     2881 ggggaaacca ggccagctcc accatcttcg acagcagcca gtcagccacg ccagtgatcg
     2941 cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc
     3001 agaacttcac tatgggcaat catgaagtgg atacccgggg tctaccgtac gggatttacg
     3061 atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag caaacgcccc cagcgggtca
     3121 ataagctgtt tagccggggg cgcggcgtcg gtgcaccact ggcgtggcag gtatggggcg
     3181 gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gctaaagaga
     3241 gttggctagc aggtgcctcg acctccggct cattgagtac gcttagctgg gcggcaacgg
     3301 gatatggata cgataatcag gcggtgggtg aaacccgtct gacgctgccg cttgggggag
     3361 cgatcaacgt taacctgcaa aatatgctgg ccagtgacag ctcatggagc aacatcgcca
     3421 gcatcagcgc cactctaccg ggaggcttta gttcgctgtg ggttaaccag gaaaaaaccc
     3481 gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca
     3541 acctgaactc actgtggtcg aagctgggca cattcagcat cagctacaat gatgaccgcc
     3601 gttacaacag ccattattac acggcagatt actatcaaaa tgtctacagc ggtccctttg
     3661 gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc aacgccaata
     3721 cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga
     3781 tgactcatca aaacggctac accatggcaa acctgtcagc acgcaagcag tttgatgaag
     3841 ggaccattcg cactgttggt gccaatctgt cacgagccat ctccggcgat accggtgatg
     3901 acaaaaccct cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc
     3961 tgaacgtcaa tagcgcggcg gacggctacg tcaataccaa tttaaccgcc aatggcagcg
     4021 tcggctggca gggtaaaaac attgctgcca gcgggcggac tgatggcaac gctggggtga
     4081 tattcaacac cgggctggag gacgacggtc agatcagcgc caaaatcaac gggcggattt
     4141 tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg
     4201 agttacagaa cagcaaaaac tcactcgaca gttacgatat cgtcagcggc cgcaaaagtc
     4261 atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta
     4321 ccgtctccgg ccgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca
     4381 accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggat gtggacaaga
     4441 aataccccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gcactggaac
     4501 tcaaccaggc gcgcggtgcc gtctgggtcg gagatgtggt ctgcagcggc ctctcatcgt
     4561 gggcggcggt gacgcagaca ggagaagaga atgagagtta acctactgat agcgatgata
     4621 atctttgcgc taatctggcc tgcaaccgcg ctcagagctg cagtgagcaa aacaacctgg
     4681 gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc
     4741 gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta
     4801 aaatacaatg gttcaggaac catctatcag caaagcctcg gctacattga taacggttac
     4861 aacaccggcc tttataccaa ctggaagttt gatatgtggc tggaaaattc accagtttca
     4921 tctcctttaa ctggcttgcg ctgcatcaac tggtacgctg ggtgtaatat gaccaccagt
     4981 cttatcctgc cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc
     5041 ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt accagtatct gcaacaaatg
     5101 cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac
     5161 gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc
     5221 acccatacga aagcagcaaa tctacggttg ataaataccc actcgctggc ggaagtattt
     5281 atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg gacgcaaacc
     5341 atcggcagcc gttcaggatt aagttgtaag atggttaact ataccctgca aacaaacgga
     5401 ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc
     5461 gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat
     5521 actgcctatt actacacctt caacgagatg aagagcgcag actcgatcta tgttttcttc
     5581 tcgagcaact tctttaagca gatggtgaac ctcgggatca gcgatatcaa caccaaagat
     5641 ctattcaact ttcgctttca gaacaccaca tcacaggagt ctggctggta tgaattttct
     5701 acctccaaca cgctgattat caaaccccgt gatttcagca tcagtattat ctccgatgaa
     5761 tatactcaga caccgtcgcg ggagggatat gttggcagcg gcgagtcggc actcgatttc
     5821 ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc
     5881 ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag
     5941 gcgaaagtac cgttcccggc gacgctttcc tttattaccc gcaacggagc tacaaaaacc
     6001 tacgatgccg ggtgcgatga tagctggcgt gatatgaccg atgcgctgtg gctgaccaca
     6061 ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg
     6121 attccaatgg ataacgccat ttcgctgcgt acggtagatg ataacggctg gtttggcgaa
     6181 gtcagcgctt caggagaaat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct
     6241 gctgacagcg gtctgtatgc tggcgacacc tttgacacag gcgatttcgg tcggcaatct
     6301 gacattttcg ctgccgtccg agactgactt tgtcagcaaa cgtgtagtga ataacaacaa
     6361 aagcgcacgg atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt
     6421 gcgcacccga ccggtggatg gtgaactgct tttcgccccc cgccagctag cgttgcaggc
     6481 tggtgagagc gagtatttta aattttacta tcatgggcca cgggataacc gcgagcgcta
     6541 ctaccgggtc tcatttcgcg aggtccccac tcgtaaccag acaagacgta gcccaaccgg
     6601 cggcgaggtc agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg
     6661 tcaggttcag tttaaatggt ccttcgacaa ggtgacagga acggtaagta acactggcaa
     6721 cacatggttt aagctactga ttaagccaga atgtgattcg accgaagagg aaggcgatgc
     6781 ctggtatcta cgtccgggag acgtggttca tcagcctgag ttacgtcagc cggggaatca
     6841 ttatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc
     6901 ttcggcggac taagacttat ccatcggtcg agggaagaat tccacctcag agccccaaaa
     6961 atcgtttaaa tgatgtggaa gcgatcgtca atatcacctg taacgatggg ctttctcata
     7021 tgcacaccat agtgttcagg agacacggaa aggtatcagg cagcctgcta acgagtggtt
     7081 taatcacgaa ttagtacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat
     7141 cgaaagttcc ccagccaacc gcggtacgtt cttacatacg atgtaccgct gttctcttta
     7201 cgatttttag ctgtactggt gaattatgag caatctgaat ccatgcatga cgtgtggtgc
     7261 ctgttgtgca tttttccgcg tctcttttta ctgggccgaa gccgacgatg ctggcggcac
     7321 tattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtaccaa
     7381 tcagaaaaac ccccgatgta ttgcccttgc aggaaccccg ggcaaaaatg cctgctgcac
     7441 gatatataaa aatcgatcgt ccacatgcag agaatttgcc atgtctggtg agaacggagt
     7501 cgtcaatgag gcttgcaatc gtgcaagggc taaatacggg ctgacaccgt tataaacata
     7561 caataattaa ttgcactgcc ccgccagcga taatagcggg gcttcgtttt tcaggggtaa
     7621 caaaacccga tacttcttct atttgccagc aacaatgcct ctcttctgta gcgttcttcg
     7681 gatctacatc atcctgaagt agcgcgatat actcactgac tatctgaatc agaatataaa
     7741 aagcaatgtt tttaacctat aaaaatggcg ctgtatttgc gccattttta tcattcaatg
     7801 cattatctgt ttgagcctaa agggatctca gggtctggct catgagtaat tctgtttcgt
     7861 aaatctctgc gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagaa
     7921 acattctcat accacgggag atcaaacaga ggtggcgtca gtcccatgag agggaatgaa
     7981 atcatatgaa caaaaagttg ggctaaagca cctgccagta agccctgcca gagtttaatt
     8041 tttggaaata cttcagcgac cacacaataa ccgacagcaa acactatcga aaagataatg
     8101 tgcgtaacac caacccagtt aaagacatgc ccggcaaagg tataaacagc cgcattggga
     8161 tctgtcagcc ccaaccagtc tcgaagaaaa atatacggtg gattgagaaa attacgcgag
     8221 caatcaattt ggcctgcagc cctgattaat gattccgggc cacacgctgc attaaacata
     8281 tccaccgggc tacgtggcgg caatggaact tcagccccac ttcacgaatg cggaaacaac
     8341 gccagcaatc agcccaatga atgcagcaag accataacgt ctgcggttcg gtggagtttg
     8401 ttcaaatata ttcatatcta ccctgcttgt accattatgt tatacacctc ttcaggagta
     8461 ttcataaaac aaggtaaatg taaagaactg tattgttttg tataacaaga tggtttccta
     8521 atcgccaatg aatataagct ccatcatttc tccctatttt tatattagaa gtgacagaga
     8581 tttgcagggt gatgcagagc tgaaaatcac aggtttcctt attggttttt gcatcgtaca
     8641 actaaagcaa taaaccagct cccatcatca tcaacatccc ggcgaacatg aatttattag
     8701 ccgggctcat agctgcattc gcgttttaag aataatcctc ctgctgtcgc cgactatgct
     8761 taacatttaa aaaagcatca gcactctcgc aacgcactct tattttcccc tttagaatac
     8821 cggaggcctg gtatgagcaa ccaaggcgaa taccccgaag ataatcgggt tgggaagcac
     8881 gagccgcacg atttaagttt gacccgtcgc gatctgatta aagtgagcgc cgcaacagcg
     8941 gcgaccgccg tggtttatcc tcattctacg ctggcggcaa gcgttccggc agctacaccc
     9001 gcgccagaga taatgcccct gacactgaag gtgaacggca aaaccgagca gcttgaggtg
     9061 gatacccgaa ccacgctact ggacgctttg cgtgaaaatc tgcatttgat cggtaccaag
     9121 aaaggttgcg atcacggaca gtgcggagcc tgtaccgtgc tggtcaatgg tcgcaggctt
     9181 aatgcctgcc tgacgcttgc agtcatgcat cagggggccg agatcaccac cattgaaggc
     9241 ctgggttcgc cagataatct tcaccccatg caggcggcct ttatcaagca tgatggcttc
     9301 cagtgcggct actgcacctc cgggcaaatt tgctcatcag tagcggcgct aaaagagatt
     9361 caggacggca ttcccagtca cgtcacggtc gatttggttt ccgctccaga aacaactgcc
     9421 gatgagatcc gtgaacgtat gagcggcaac atctgtcgct gtggtgcata cgctaacatc
     9481 cttgccgcca ttgaagatgc tgcggaggag ataaaatcat gaaggcgttt acctatgaac
     9541 gagtgaacac cccagccgag gcggcactta gcgctcagcg cgtacccggc gcaaaattta
     9601 tcgcgggcgg gaccaatctg ctggacctga tgaagctgga aattgaaacg cccacccacc
     9661 ttatcgatgt gaacggcctc gggctcgata agattgaagt gaccgacgcg ggtgggctgc
     9721 gcatcggcgc actggtacgg aacaccgacc tggcggctca cgagcgcgtg cgtcgtgatt
     9781 acgcggtact ctcccgcgcc ctgctcgctg gcgcatctgg tcagttacgt aatcaggcaa
     9841 ccaccgcagg taatctgctc cagcgcacgc gctgccccta tttttacgac accaatcagc
     9901 cctgcaataa gcgcctgccc gggagcggct gcgcggcgct tgaaggcttt agccgtcagc
     9961 acgcggtggt aggcgtaagt gaagcctgca tagccaccca tccgagcgat atggcggtcg
    10021 caatgcggtt gctggatgcg gtggtggaaa ccatcacgcc ggagggaaag actcgcagta
    10081 tcacactggc tgatttttat cacccaccgg ggaaaacgcc gcacattgaa accgccctgc
    10141 ttcccggtga gcttatcgtt gcggtgacgt tacctccacc gctcggcgga aaacatatct
    10201 accgtaaggt gcgcgatcgc gcctcctacg cctttgccct ggtatcggtc gcggcgatta
    10261 ttcagcctga cggcagcggg cgcgtcgcgc tgggcggagt agcacataag ccctggcgca
    10321 ttgaggctgc ggatgctcag ctatcctagg gggcgcaggc cgtatatgac gcgctgttcg
    10381 ccagcgccca tcccaccgct gaaaatacct ttaaactcct gttggcgaag cgaacgcttg
    10441 cctccgtact ggctgaagcg agggcacagg catgaaattt gataaacccg caggggaaaa
    10501 cccgatcgat cagctgaagg ttgtcggtcg tccccatgac cgcatcgacg gaccgctgaa
    10561 aactaccggc acggcacgct acgcctacga atggcatgaa gaagccccca acgccgccta
    10621 tggctatatc gtcggttccg ccattgccaa aggacgcctc accgcccttg atacggacgc
    10681 cgcgcaaaaa gcgccgggcg tactggctgt cattaccgcc agtaacgccg gggcactcgg
    10741 caaaggcgac aaaaacaccg ccagactgtt aggcggcccc actattgagc actatcatca
    10801 ggccattgcg ctggtagtgg ccgagacctt cgaacaggcg cgagcggcgg cctcgctggt
    10861 gcaggcacac tatcgccgta ataaaggagc ttactccctg gcggacgaaa aacaggccgt
    10921 caatcagccg ccggaagaca cgcccgacaa aaacgtcggt gactttgacg gggctttcac
    10981 ctccgctgcg gtgaagattg atgctaccta cacgaccccg gaccagagcc atatggcgat
    11041 ggagccgcat gcctcgatgg ccgtctggga tggaaataag cttaccctct ggacctcaaa
    11101 tcagatgatt gactggtgcc gcaccgatct ggcaaaaacg ctgaaagttc ccgtggagaa
    11161 tgtgcgtatt atctccccgt atatcggcgg agggtttggc ggcaagctgt tcctgagaag
    11221 cgatgcgctg ctggcggccc tcgccgcccg agcggtgaaa cgtccggtta aagtgatgct
    11281 cccccgcccc tctattccca ataacaccac gcaccgcccc gccacccttc agcacttgcg
    11341 tatcggtgcc gaccagagcg ggaaaatcac cgctatctca catgaaagct ggtctggaaa
    11401 cctgcccggc ggcacgccgg aaacggcggt acagcaaagc gaattactct acgccggggc
    11461 gaatcgtcat accggcctgc ggctcgccac gcttgatttg ccggaaggga acgccatgcg
    11521 tgcgcccggc gaagcccccg gtctgatggc gctcgaaatc gcgatcgacg aactggcgga
    11581 aaaagcgggc atcgatcccg tcgagtttcg catcctgaat gacactcagg ttgaccccgc
    11641 cgacccgacg cgctgcttct ctcgccgtca gcttatcgag tgcttgcgca ccggagcgga
    11701 taaatttggc tggaagcagc gcaacgccac acccggacag gtgcgcgacg gggagtggct
    11761 agtcggccac ggtgttgcgg cgggctttcg caataatctg ctggaaaaat cgggtgctcg
    11821 ggttcacctc gaacaaaacg gcaccgttac cgtagaaacg gacatgaccg acattggcac
    11881 cggcagctac accattctgg cccagacggc agcggaaatg cttggcgtac cgctggagca
    11941 ggttgcggtt cacctcggcg attccagttt cccggtttct gcgggttctg gaggacaatg
    12001 gggcgcgaat acctccacct ccggcgttta cgccgcctgt atgaagcttc gcgaaatgat
    12061 tgcctcggca gtcgggtttg atcctgagca gtcgcagttt gccgacggca agattaccaa
    12121 cggtacccga agcgccacgc tacatgaagc caccgcaggc ggcagactga cagcggaaga
    12181 gagcattgaa ttcggaacac tgagcaaaga gtaccagcag tcgacctttg ccgggcattt
    12241 tgtggaggtc ggcgtgcata gcgcgacggg agaagttcgg gtccggcgta tgctcgctgt
    12301 gtgtgctgca ggacgcatcc tgaatccgaa aactgcgcgc agccaggtca ttggcgcaat
    12361 gactatgggc atgggcgcgg cactgatgga ggagctggcg gtggatgacc gtttgggcta
    12421 cttcgttaat cacgatatgg cggggtatga ggtgccggtc catgcggata tcccaaaaca
    12481 ggaggtgatt ttcctggatg ataccgaccc catatcctcc ccgatgaagg ccaaaggtgt
    12541 cggtgagctg ggcctgtgcg gcgtgagcgc ggctatcgcc aacgcggtgt ataacgccac
    12601 cggtattcgg gtacgggatt atcccatcac tctggataag ctgctcgata agctgccgga
    12661 tgtggtttaa ggagaaacaa tgtcataccc gctttttgat aaagacgaac actggcataa
    12721 gccagagcag gcgtttctca ccgatgacca cctgaccatt ctgcgcttcg ccgtagaggc
    12781 gctaatgtcc ggtaaaggag cggtgctggt gacgctggtg gagatacgcg gcggcgcagc
    12841 gcgcccgctc ggggcgcaga tggtggttcg cgaagatggt cgttactgcg gttttgtctc
    12901 tggcggctgc gtggaggccg ctgccgcttt tgaagcgctg gagatgatgg gctcaggccg
    12961 cgatcgcgaa attcgctatg gcgaaggttc gccgtggttt gacatcgttc tgccctgcgg
    13021 cggtgggatc acgctgacgc tccataaact acgctcggca cagcctctgc tcgccgtgct
    13081 gaaccgtctg gaacagagaa aaccggcggg gctgcgctac gatccgcaag cacaatcgct
    13141 ggtgtgcctg cccacgcaaa cccgaacggg ctggaatctc aatggctttg aggtggggtt
    13201 caggccatgc gtcaggctga tgatttacgg acgttctctt gagacgcagg caactgcgag
    13261 tcttgcagca gccacaggct atgacagcca tatcttcgat ctttttccgg cctcagccag
    13321 cgctcagatc gataccgata cggcggtcat tttgctgtgc catgatctca accgggagct
    13381 gccagtgttg caggccgcgc gagaagcaaa acccttttat ctcggcgcat tgggcagcta
    13441 tcgaacccac acgttacgtc tgcaaaagct ccacgagctg ggatggtcca gggaggaaac
    13501 aacgcaaatc cgggcacccg tcgggatatt ccccaaagcc cgggatgcgc atactctggc
    13561 actctccgtg ctggcagaag tcgcctctgt acgtctccat caggaggagg attcatgcct
    13621 gcccccgtcg tcctgatcct tgcggccggg cgtggagagc gctttctcgc ctccggggga
    13681 aatacccata agtgtatcgg ctggcgtcag tccccggagg ttgcgcctta tcgctggcca
    13741 tttgaagaaa acgggagaac tttcgacctt gcgattgaac cgcagattac gactaatgat
    13801 ctgcgtctga tgttgaggct ggctcttgcc ggcggaggaa taacaattgc cactcaggaa
    13861 actttcaggc catatattga aagcggtaag cttgtatcgc tgcttgatga ctttcttcca
    13921 caatttccgg gcttctatct gtatttccca cagcgtcgca atattgcacc aaagctccgc
    13981 gccctgattg actacgtcaa agaatggcgg cagcaattgg tttaaatagc tgcacctgca
    14041 ctgcctgatg tcagaacagt attttgatga attgccaagg ttacaatggc acaaatgcgg
    14101 cacaggagga aacgtggtgt atttaaatat ggggtaactc tttgatttta atggtgccga
    14161 taataggagt cgaacctacg accttcgcat tacgaatgcg ctgctctacc aactgagcta
    14221 tatcggccct gaaaggacat gttcacgaac gtgaatcacg gtggacaagg ttaaaactaa
    14281 ccgggcgatg cgtcaatggc cttgtgaatc aaatggctac ttttgcatca cccggtttta
    14341 tttacgcacg aatggtgtaa tcaccaatgc cgatccactt gtaagtggtc agtgcttcca
    14401 gccccattgg gccacgcgcg tggagttttt gtgtgcttac cgccacttcc gcacccagac
    14461 caaactggcc gccgtcggta aaacgcgtag aggcgttaac gtaaacagcg gacgaatcca
    14521 cttcgttaac aaaacgctgg gcgttgcgca tatcgcgggt caggatcgca tcggagtgtt
    14581 gtgtgccgtg ttcacgaata tgggcgatgg catcgtcaag atcgctgacg attttgacgt
    14641 tcaaatctaa tgacagaaac tcatcgtcat actcttcggc tttaacagca accaccttcg
    14701 cagggcctgc ctgcaactgc gccagtgcag ctgcatctgc gtgtaatgtc acgccgcttt
    14761 ccgccatttg tttgcttaat gcgggcagga agctatcggc gatgttttta ttcaccagca
    14821 acgtttcaac cgtattacat gtgctcggac gctgagtttt cgcgttgacg atcactttta
    14881 atgcttcagc gatctctaca ctttcatcaa cgtaaatatg gcatacgcct ataccacctg
    14941 tgatcaccgg gattgtcgac tgttcacggc acagtttatg caaaccagcg ccaccacgcg
    15001 ggatcagcat gtcgatgtat ttatccatac gcagcatttc actgaccagc gcacggtcag
    15061 gattatcaat cgcctgcacg gcacccgccg gtaagccgca ggatttcagg gcgtcctgaa
    15121 tcaccgccac cgttgcagcg ttagtgcgac acgtttcttt gccaccgcgc aggatcaccg
    15181 cattaccggt tttcaggcac agcgaagcga catcaaccgt cacgttcggg cgcgcttcat
    15241 aaatcacgcc aataaccccc agcggtacgc gacgacgctc aagacgcagg ccgctgtcca
    15301 gtacgctgcc atcgattacc tgccccaccg gatcggcgag gttacacacc tggcgcacat
    15361 catcggcaat gcctttcagc cgtgcgggcg tcagtgccag acggtcaagc atcgcttcgc
    15421 caaggccatt ggcacgcgcg tcagcaacat cctgggcgtt agcgttgagg atgatttcgc
    15481 tttgtgcttc cagttcatcg gcgatttttt ccagcacgcg atttttttcg cggctggaga
    15541 gttgcgctaa tttatacgag gcttgcttcg cggcaatgcc catttgttcc agcatcagcc
    15601 tgctccttaa cgggtaatca tgtcatcacg gtgaacggca accgggccgt attcatatcc
    15661 cagtattgca tcaatttctt gcgagtggtg cccggcaata cggcgtaatg catcgctgtt
    15721 gtaacgactg acgccgtggg cgatatcgcg accttcgagg ttgcaaatgc ggatgacttc
    15781 accacgcgag aaattgccag tcacgctttt aatgcctttc ggcaacaggg agctgccgcg
    15841 ttcaagaatg gcggcagttg ccccttcatc taccgtgatt tcacccgccg gcggcgcacc
    15901 gaaaatccag cgtttacggt tttcaagcgg agtcgcctgg gcatggaaca gcgtaccgac
    15961 ggaaatgcct tccatcacat caccaataac gcccggcttg ctgcccgcgg caataatggt
    16021 gtcgataccc gcacggcaag ccacgtcagc ggcctgcaat ttggtactca tgccgccagt
    16081 tccgaggcct gaaacgctgt caccggcaat cgcgcgcagt gcgtcatcaa tgccgtaaac
    16141 atctttaatc agttctgcct gcggattgct gcgcggatca gcggtataca aacctttttg
    16201 atcggtcagc agcaacagtt tatcggcacc cgccagaatc gccgccagcg cagaaaggtt
    16261 atcgttatcg ccgaccttaa tctctgccgt agcgacagca tcgttctcat tgattaccgg
    16321 aacgatattg ttatcgagca acgcacgcag ggtgtcgcgg gcgttcagga agcgttcacg
    16381 gtcttccata tcagcacggg tcagcagcat ctgcccgacg tgaatgccat aaatcgaaaa
    16441 cagctgttcc cacagttgaa tcagtcgact ctgccctacc gccgccagca gttgtttcga
    16501 ggcgatagtc gctggcagtt ccgggtaccc caggtgctca cgtccggcgg cgatcgcgcc
    16561 cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac tgcgcgcact ggcgaacaag
    16621 ttcaacgata tgggcacggt tcagacggcg cgatccgcct gttagcacac tggtgccgag
    16681 ttttaccacc agcgtctggc tgtcactcat gattctctgc cattcaattt taggaaaaat
    16741 gatatcaaac gaacgtttta gcaggactgt cgtcggttgc caaccatctg caagcaaagc
    16801 atggcgtttt gttgcgcggg atcagcaagc ctagcggcag ttgtttacgc ttttattaca
    16861 gatttaataa attac
//
LOCUS       DAZGCS010000104        16798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGCS010000104 DAZGCS010000000
VERSION     DAZGCS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.104"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..297)
                     /locus_tag="ACHK01_003788"
     CDS             complement(145..297)
                     /locus_tag="ACHK01_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8098666.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(557..1291)
                     /gene="cysH"
                     /locus_tag="ACHK01_003789"
     CDS             complement(557..1291)
                     /gene="cysH"
                     /locus_tag="ACHK01_003789"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8098667.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1365..3077)
                     /gene="cysI"
                     /locus_tag="ACHK01_003790"
     CDS             complement(1365..3077)
                     /gene="cysI"
                     /locus_tag="ACHK01_003790"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO8098668.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3077..4876)
                     /gene="cysJ"
                     /locus_tag="ACHK01_003791"
     CDS             complement(3077..4876)
                     /gene="cysJ"
                     /locus_tag="ACHK01_003791"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO8098669.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5195..5557
                     /gene="queD"
                     /locus_tag="ACHK01_003792"
     CDS             5195..5557
                     /gene="queD"
                     /locus_tag="ACHK01_003792"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO8098670.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5635..6906
                     /gene="ygcN"
                     /locus_tag="ACHK01_003793"
     CDS             5635..6906
                     /gene="ygcN"
                     /locus_tag="ACHK01_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8098671.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6897..7157
                     /gene="ygcO"
                     /locus_tag="ACHK01_003794"
     CDS             6897..7157
                     /gene="ygcO"
                     /locus_tag="ACHK01_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8098672.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7174..7749
                     /gene="ygcP"
                     /locus_tag="ACHK01_003795"
     CDS             7174..7749
                     /gene="ygcP"
                     /locus_tag="ACHK01_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO8098673.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7897..8757)
                     /gene="ygcQ"
                     /locus_tag="ACHK01_003796"
     CDS             complement(7897..8757)
                     /gene="ygcQ"
                     /locus_tag="ACHK01_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8098674.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8754..9533)
                     /gene="ygcR"
                     /locus_tag="ACHK01_003797"
     CDS             complement(8754..9533)
                     /gene="ygcR"
                     /locus_tag="ACHK01_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO8098675.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9511..10920)
                     /gene="ygcS"
                     /locus_tag="ACHK01_003798"
     CDS             complement(9511..10920)
                     /gene="ygcS"
                     /locus_tag="ACHK01_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8098676.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGISLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10942..12396)
                     /gene="ygcU"
                     /locus_tag="ACHK01_003799"
     CDS             complement(10942..12396)
                     /gene="ygcU"
                     /locus_tag="ACHK01_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO8098677.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12466..13251)
                     /gene="ygcW"
                     /locus_tag="ACHK01_003800"
     CDS             complement(12466..13251)
                     /gene="ygcW"
                     /locus_tag="ACHK01_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8098678.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13570..14847
                     /gene="yqcE"
                     /locus_tag="ACHK01_003801"
     CDS             13570..14847
                     /gene="yqcE"
                     /locus_tag="ACHK01_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO8098679.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14874..16352
                     /gene="ygcE"
                     /locus_tag="ACHK01_003802"
     CDS             14874..16352
                     /gene="ygcE"
                     /locus_tag="ACHK01_003802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO8098680.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         4051 a         4387 c         4193 g         4167 t
ORIGIN      
        1 atgaataaat taatgatttt catttgaaat tcataataat aaatagagac tatatattca
       61 cataaaaaat ataaatttta aaaaagggtg caggagacca aaccatcaca attcagtacg
      121 cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa ctgccttaaa
      181 ctccatacca cgttctctga tactcaactc acacaacgag tcacctacca tcagcgtgaa
      241 tcccagtact gtacaacaca gtacgatgat tgccacaagg gcatattttg tcagcatgtc
      301 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgtaag cctcaggttg
      361 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
      421 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
      481 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
      541 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
      601 gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta tcgcccaccg
      661 ataaatatcc ttcgtcccat aatgggtgat atttcaggcc atgtttttgc agatactgat
      721 atatcgttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
      781 ccagcaccgg taaattggcg cggctgccgg attgttcgcg gcgcaggcca gcaaaccacg
      841 tttgcgcatt cagttctttc agcgcccggt tcatcggttc gactttgttg atttcattgt
      901 acttttcaat gccttcaacg ccctgttccc acagtttgcc gtagcgtgct tcctgccagg
      961 cggcgctttc ggtagcgcgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     1021 caataaagcg gtaggtttcc gggaacagat agccggtgtc ggtgaggatc accggaatat
     1081 ccgggtgaat ctgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     1141 aaagtacata ttcaccgggc agattatcca gcgcccaggc aacgcggcct tcagcgtcca
     1201 gtttttccag ttcggcgtta gtttctgcca gcgccagaat gcgatctacc ttcggcagtt
     1261 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     1321 cgacgctcac gcgtcttatc aggcctacaa gaccgggctg atggttaatc ccacaaatcg
     1381 cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc gccgaagcct
     1441 tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga cgccaggatt
     1501 tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat gcggttgccg
     1561 ccaagatgca gattgtagcg acccggtgct ttacccacca ggcccacttc cgccagcatc
     1621 gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc atcgctgaca
     1681 ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa acgctctgct
     1741 tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt ttcacgctgc
     1801 ggcgtgacgg cattcattaa accgctctct ttggcgatct tctcgatctt cgctttctcg
     1861 ctttccggta caccggcgat gatcagattc tggttcgccg taatgcggaa atcgcctttg
     1921 tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata atcaaggatg
     1981 cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt aacccagcca
     2041 atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt gatccccgcg
     2101 cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt gtatttggtt
     2161 ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac gacggcttcc
     2221 gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt gcgggcgtag
     2281 gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt gaagcctacc
     2341 agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag atcgatatcg
     2401 ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt ctgaccaagg
     2461 atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc cgcatacgcg
     2521 cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc ttccgcgtgc
     2581 agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc gttagctgtc
     2641 gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac gttctttttc
     2701 agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc atagatggtg
     2761 ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac cccacccggc
     2821 agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc acgttcggcg
     2881 cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag gaagttatcg
     2941 cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc gcgcaggtag
     3001 ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac taaaggccct
     3061 ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg cgcagctcac
     3121 ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc acttccagaa
     3181 gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa atgtgggcac
     3241 catcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct tgtacgtaaa
     3301 ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg tgtcagcacg ccatctttga
     3361 cgtagcgctg ccactccacc tggtacagga agtcttccgt aaagtgcgga ttaccaaaga
     3421 acagccagtt tttacctggc gcttcgtcgg cggcgcgttg ctgcataaag gcgcggaacg
     3481 gcgcaatacc ggtgcctggg ccaatcataa tcaccggggt ttccgggtta gcgggcaggc
     3541 gaaagttatc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc acgcgatccg
     3601 cgaggaagct ggaggcacca ccggcacggg ctcggccttc cacgtcgtaa cgcaccacac
     3661 caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg gaatacaggc
     3721 gcggggtcag cgggcgcagc agattaatca gcgcttcggc atccagttgc gccggagaga
     3781 aacgcaccat gtcgacaatc ggcgtcgtcg cggcgtaatg ctgtaacttc gctttatcgc
     3841 ccaccagcgg cagcagtgtt tcactgcggg taagcgtggc gtaattctca acaatgttgg
     3901 cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc aacgttttgc
     3961 cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt tctttcacca
     4021 gtgccggatc gttctgatac cagacgccca gcgcgtcacc cggctggtaa cgcaggcccg
     4081 agtcacctaa gtcaatttcg atatggcgaa cgtctttttc agagttacgt ccggtaattt
     4141 tctggttaac cgaaaggctc gccaccagcg gagcgtcttt gctgtacggg ctggagtgga
     4201 tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc gcacgcgatt
     4261 taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat tcaacatcgg
     4321 catcgacacg gtcgagcagg cgttcaccgc ccagttccgc cagcttgctg tcaaaatctt
     4381 tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg gcaaatgcgg
     4441 tgttttccag cttcggcgct tttttggaga acaggaactt atgcagcgcg acggcttctt
     4501 ccggcggttc cccttctcct tgcgttgacg tcaccacgat aagcagtttt tcgctggcga
     4561 tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt gctgctaata
     4621 aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgag gcggagataa
     4681 tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca gcaggctgct
     4741 gattgagtac gccccagaaa tagccagaaa cccaggcaag ctgggtggga gttaaatcgg
     4801 tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac gcggaaggtg
     4861 ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct gcgccctgtc
     4921 agcgcccata aaacagaaga gaaggtaagg ttaacggggt gaacggtgtg gattaaagac
     4981 gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt ctttaacaac
     5041 aaaatagatt aaccaaccta atgaaaaaca aatgaattta gccaatcatt aagataaatc
     5101 agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt tccggtactc
     5161 tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat ttaaagattt
     5221 caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat gtggtcgcct
     5281 gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc cgcatacggg
     5341 ctggattatc gatttcgctg aactaaaagc ggcgtttaag ccaacttatg agcgcctcga
     5401 tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag ttttagcaaa
     5461 atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga tggtaaaaga
     5521 aacctgcacc gctggttgta tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt
     5581 tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca tgacatggaa
     5641 gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc
     5701 tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa
     5761 aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc gcaatttcat
     5821 ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt aacgcccgat
     5881 ggcgcaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt attacgtgca
     5941 cggttcgatc cgtggctggt tgccgaagcc gaagaagaag gtgtcgaatg catccccgga
     6001 gcgacggtgg atgcactgta tgaagaaaac ggcagggtgt gtggtgtcat ttgtggtgac
     6061 gatattctcc gtgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt cctggctgag
     6121 cgtcacgggt tagtgactcg tcctgctggt gaagcgatgg cgttggggat caaagaagtg
     6181 ctgtcgctgg aaccatccgc tattgaagaa cgttttcatc tggagaataa cgaaggcgca
     6241 gcgttgctgt tcagcggcag gatctgtgat gacttacccg gcggcgcatt tctttatact
     6301 aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac gcaaagtcgt
     6361 gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc
     6421 aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg cttgcacagt
     6481 atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt gcgcagttgc
     6541 gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc gcaggcggcg
     6601 gcacaaacgc tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt tgcgctttat
     6661 catcacaacg tagagcgcag cctgctatgg gatgttctac aacgttatca gcatgttccg
     6721 gcgcttttgc aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca ggatatttcc
     6781 cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt attctggcgt
     6841 catttacgtc gtcatggcct gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt
     6901 ctgtagccca taatctctgg cgcgttgctg atgcgccgca cattgttccg gctgactccg
     6961 ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggcaggtctt ttttcgctca
     7021 caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc acctgccgtt
     7081 tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga ttcggcatca
     7141 cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct gctccgccag
     7201 aatccggtga ttgctgccgt taaagataat gccagcctgc aactggcaat cgactctgaa
     7261 tgccagttta tttccgtgtt gtacggcaat atctgcacca tcagtaatat cgttaaaaag
     7321 attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg cgcgtcaaat
     7381 aaagaagtcg tgattcagtt tttgaaactg gtgaccgagg cggacggcat tatcagtacc
     7441 aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca tcgcctgttt
     7501 attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc gaatccggat
     7561 tgtattgaga tcctgccagg ctgtatgccc aaagtgctgg gctgggtgac agagaaaatc
     7621 cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg taatgcgatt
     7681 aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt agcgaaaaaa
     7741 ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg ccgcacgcca
     7801 tatccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct acaagtcccc
     7861 aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg cgtgaatgtt
     7921 tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat ctgcctgtga
     7981 aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat tgcgtacgcc
     8041 cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag gtgccagcag
     8101 atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg
     8161 actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct ggttatctgc
     8221 ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt cgcgagtaac
     8281 atttttcaga tcctctgtgc taaccagcca gtcgggaagc gcacccggga caatgttcag
     8341 ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac gcgccaggga
     8401 aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga
     8461 tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc ttgcaccatg
     8521 taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg gaaagagaac
     8581 aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct gttccgccac
     8641 taccggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat cctgcgccgc
     8701 cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga tattcatggc
     8761 tgcatccttt ggcgtaaata gtcctgccat agcttttgag ctttttctgc taccgtttgc
     8821 ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag acactgcatc
     8881 gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc agccattcgc
     8941 tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc aatcaccgca
     9001 ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag ggtgataaac
     9061 agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag catttccgcc
     9121 agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt aattatcaga
     9181 tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc cggcgcaaag
     9241 cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc catgagatag
     9301 cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga catcggcgta
     9361 ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc accgagtaaa
     9421 cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg ccactctttt
     9481 tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt cattcgcacc
     9541 tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc atagccaggt
     9601 cacaacaaaa ccaaccaaca acactgtcgc caggagcaat aaggtgactt gcattcccca
     9661 ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc gactcatggc
     9721 ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg caggcaaaat
     9781 gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa gcagcagcgt
     9841 taatgaactg ccggaaggca aacaggccat gactaccagc gttgccgcca gcagcaaaaa
     9901 acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc ccaacagcgc
     9961 gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagaccaat
    10021 agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta cgaggcagac
    10081 aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca aggttttgat
    10141 gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac caaaatagcg
    10201 atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggtgattc
    10261 tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg atgccagtag
    10321 ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac ttgccagcac
    10381 atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat ggcggcgcgg
    10441 ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac taatgccaat
    10501 caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta aaaacgaagc
    10561 aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat ggtcggagat
    10621 ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc cgatcattcc
    10681 cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt aaccaatgac
    10741 gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa tgcggcagtg
    10801 aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc
    10861 attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccccgtcat
    10921 cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg tattcataat
    10981 gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc
    11041 cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccgcc
    11101 gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact tgtcgatttc
    11161 ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt tggtgccgtt
    11221 ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt gcgggaactc
    11281 agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga
    11341 cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac
    11401 tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg
    11461 ctggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat
    11521 gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtctgcaa aatgggtgaa
    11581 gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac
    11641 catgacttca cgcaggatgt tgaagccggt tttcatgtct tccaggatat agccgtagaa
    11701 gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga tatagcaaag
    11761 tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc ggcgtggcac
    11821 gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa cgaccatatc
    11881 ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaggccgcc
    11941 catctgagcc agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa
    12001 cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca tattctcaat
    12061 atcaatatta atgatttgat tcatggcgga gccgtcgagc accaccgagt tttccacaac
    12121 ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt taattttgtg
    12181 cgcattcata aaattcagca cacgggagac ttgttctgtg gaaccgagtt ttacgaccgc
    12241 tgccggaatc ggcaaagtat aaataccatg aatatccgga aatttacgaa aacggtcaat
    12301 actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt
    12361 tagctggtcg acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa
    12421 atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc gcactaaata
    12481 accaccatcg accactaata aatgcccatt gacataattt gaagccggac ttgcgaggaa
    12541 tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag
    12601 aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg ttgcataata
    12661 gccaggggcg ataccattta cctgaatatt atattgacct agctcatcac aataagcttt
    12721 ggtgaacccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt gtccacctaa
    12781 gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga tcataatttt
    12841 tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcggatccca
    12901 gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgtttaccag
    12961 aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg gcgcgccttc
    13021 tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa tcatttcctt
    13081 tgtttcgccg ttatctttga cgaaactagg aataaagata tttgcgccag ctttggccaa
    13141 cgccatggca aatgcctggc ctaaaccgct attcccaccg gtaacaattg cggttttacc
    13201 tttcagtgag aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc
    13261 caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    13321 tctcattatc tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga
    13381 tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta
    13441 ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag
    13501 aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg
    13561 gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt
    13621 agcggcggcg ttagtttcga cctggcttat ttacgttata tttatcaaat tcccatggcg
    13681 aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg
    13741 gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg
    13801 attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg
    13861 ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg
    13921 tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg
    13981 ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg
    14041 gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac
    14101 agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac
    14161 ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt
    14221 ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac
    14281 gccattctga gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca
    14341 gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc
    14401 ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgatcca aatcctttcc
    14461 gtactcggcc tgctggcgtt aactgccctg ctcgtcacga actctaaccc gcaatcggtc
    14521 gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg
    14581 tactgggcct gccctggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt
    14641 atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg
    14701 caagacaccc tgcccgcagc agaagcctac cgcaatatgt ggctgatggg aatggcggcg
    14761 cttgccatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc
    14821 gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga
    14881 aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc
    14941 tggaaggtaa cgtggtttgc gaaggcaaag gcttattaca gccgatgcac acgccagatg
    15001 ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt gccggtcacg
    15061 atttgatgag tcagtttgct gggaataaag aagatattgt cggtattggt ctgggatcca
    15121 tccgttgctg tcgtgcgtta ttgaaagccg atggcacgcc tgctgcgccg ttgattagct
    15181 ggcaggatgc acgcgttaca cgcccttacg aacacactaa tcctgacgtg gcatatgtca
    15241 cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac aatatcgcca
    15301 actattttgg tcagtggccg gtggattata agagctggac atggagcgaa gatgctgcgg
    15361 taatggataa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg cctggcaccg
    15421 tcctcggaca tatcacacca caagccgcac tggcgacaca tttcccggca ggactgccgg
    15481 ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta ctggatgatg
    15541 aaactgcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagcactgc
    15601 cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg
    15661 aaggttacgg tattcgcaaa ggtatgtgga cggtgagctg gctgcgtgac atgttaggcg
    15721 agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga
    15781 aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca
    15841 atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg
    15901 catggatata tcgttcgata ttggaaagcg tggcgctaac gctgaagaac aattacgaca
    15961 atatgtgtaa tgaaatgaat cactttgcga agcatgtgat cattactggc ggcggttcga
    16021 acagcgatct gtttatgcaa atttttgccg acgtgttcaa ccttccggca cgccgtaacg
    16081 ccattaacgg ttgtgcaagt ctgggagcag cgattaatac agcggtaggt ctggggctat
    16141 acccggatta cgcaacggct gttgataaga tggttcgcgt gaaagatatc tttataccga
    16201 ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg cattttcaaa gacctaacca
    16261 aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg
    16321 tggattcgat ccagagctgg tcgaatgcgt aagtgaggaa ggccgggcgg gaaactgccc
    16381 ggcctgaaca tacctgaatg gttatccccg ctgacgcggg gaacataagt tcgacaactt
    16441 aaaaaatgat aacaagtccg gtttatcccc gctgatgcag ggaacataat aaaaacttta
    16501 actagttcgg tttatccctg tttttacaag gaatattaac tacactcgat gtctttagaa
    16561 taaaagacaa tacgaatcgc actttatcaa ttttcactta aagtatgaac tggatatctt
    16621 tattcataag aactattcat caacagcgta tagaggcggt tatgtaaaac cactcattag
    16681 cctcaaaaca aagacaaact tccagattta ttacttaacc aatgaataac aagcttaaaa
    16741 aacatactgt tattctaaag aaaaaattaa caggcattaa ttcaatatta aaaaaata
//
LOCUS       DAZGCS010000105        16785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGCS010000105 DAZGCS010000000
VERSION     DAZGCS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.105"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..604
                     /locus_tag="ACHK01_003803"
     CDS             41..604
                     /locus_tag="ACHK01_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098681.1"
                     /translation="MQNTSKEINGGCLMVINYKQLREKREQVKESFRRNEDLTPLVRL
                     AQGIVDAYEISLELPSQTWTDSDGNRQHYVSCGLEAAEGFRRMPLSQIPAATPKARGS
                     NDERKLTFSIETVVDDTPGEVAFVHTPVSIAMYNDEIQVRVNNNIVPLKEGNSPYTTV
                     CEAIQYYVLSEIDNLKPDGTQKMVQLW"
     gene            complement(619..1665)
                     /locus_tag="ACHK01_003804"
     CDS             complement(619..1665)
                     /locus_tag="ACHK01_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8098682.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(1665..3416)
                     /locus_tag="ACHK01_003805"
     CDS             complement(1665..3416)
                     /locus_tag="ACHK01_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO8098683.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFMVMEF"
     gene            3571..4407
                     /locus_tag="ACHK01_003806"
     CDS             3571..4407
                     /locus_tag="ACHK01_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8098684.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            4431..5483
                     /locus_tag="ACHK01_003807"
     CDS             4431..5483
                     /locus_tag="ACHK01_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO8098685.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLAVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            5529..6329
                     /gene="gpM"
                     /locus_tag="ACHK01_003808"
     CDS             5529..6329
                     /gene="gpM"
                     /locus_tag="ACHK01_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO8098686.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     IANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            6431..6925
                     /locus_tag="ACHK01_003809"
     CDS             6431..6925
                     /locus_tag="ACHK01_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8098687.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            6925..7125
                     /locus_tag="ACHK01_003810"
     CDS             6925..7125
                     /locus_tag="ACHK01_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO8098688.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            7128..7451
                     /locus_tag="ACHK01_003811"
     CDS             7128..7451
                     /locus_tag="ACHK01_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8098689.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            7448..7840
                     /locus_tag="ACHK01_003812"
     CDS             7448..7840
                     /locus_tag="ACHK01_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO8098690.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGNQVSWEWPLYEKIAAAFRQASQEL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            7837..8232
                     /locus_tag="ACHK01_003813"
     CDS             7837..8232
                     /locus_tag="ACHK01_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO8098691.1"
                     /translation="MKLWPTLGVAFLLITSMRLSWSLGRENARNEAQASTLKSTVDTL
                     NIISAGVQDMQQVLAQLRAENQQRNQDGEVRREQLRNDIAKDECAHALPDARFTDRLR
                     RHAERARASAVSPAYTADADHAGNASPLP"
     gene            8371..10248
                     /locus_tag="ACHK01_003814"
     CDS             8371..10248
                     /locus_tag="ACHK01_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8098692.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFTAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRGIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSE
                     GVFKITKQSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVA
                     LPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFT
                     AGSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVDIGSLVLEINNPA
                     A"
     gene            10272..10739
                     /locus_tag="ACHK01_003815"
     CDS             10272..10739
                     /locus_tag="ACHK01_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8098693.1"
                     /translation="MLKTNSLRESMLHGCRWCQANPEKFTIFVESGNIETTGEAPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            10732..11367
                     /locus_tag="ACHK01_003816"
     CDS             10732..11367
                     /locus_tag="ACHK01_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8098694.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTEWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            11379..11945
                     /locus_tag="ACHK01_003817"
     CDS             11379..11945
                     /locus_tag="ACHK01_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8098695.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            11942..12292
                     /locus_tag="ACHK01_003818"
     CDS             11942..12292
                     /locus_tag="ACHK01_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO8098696.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            12296..13192
                     /locus_tag="ACHK01_003819"
     CDS             12296..13192
                     /locus_tag="ACHK01_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8098697.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            13185..13715
                     /locus_tag="ACHK01_003820"
     CDS             13185..13715
                     /locus_tag="ACHK01_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO8098698.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            13718..14431
                     /locus_tag="ACHK01_003821"
                     /pseudo
     CDS             13718..14431
                     /locus_tag="ACHK01_003821"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            14996..15850
                     /locus_tag="ACHK01_003822"
     CDS             14996..15850
                     /locus_tag="ACHK01_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8098699.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTPDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPGSGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNEENTVKNIA
                     FNYIVRLA"
     gene            15850..16428
                     /locus_tag="ACHK01_003823"
     CDS             15850..16428
                     /locus_tag="ACHK01_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8098700.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGYTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTSWVKDAEAEKLFRSQEAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVDTTVAADIEWPVAPQ"
     gene            complement(16472..>16785)
                     /locus_tag="ACHK01_003824"
     CDS             complement(16472..>16785)
                     /locus_tag="ACHK01_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO8098701.1"
                     /translation="LGANIGKGLTIPHHAGIVVHFAVDAGENLVLRQNTTIGQIDGDI
                     PGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTYITKKSGVVLY
                     K"
BASE COUNT         4164 a         4513 c         4549 g         3559 t
ORIGIN      
        1 cgcgtcagcg atacggcacc ttgccgacca tacttcataa gtgcaaaata cgagcaaaga
       61 aatcaatgga ggctgtctta tggtcattaa ttacaagcag ttaagagaaa aacgggagca
      121 ggtaaaggag agctttcgcc gcaatgaaga tctgaccccg cttgtacgcc ttgcccaggg
      181 cattgttgat gcttatgaaa tctcgctgga gctgccatca cagacctgga cagatagcga
      241 cggtaatcgc cagcattacg tttcatgcgg actggaagca gccgaaggat ttcgcagaat
      301 gcctttatcc cagatccctg ccgctacccc caaagcacgg ggcagcaatg atgagcgaaa
      361 actgactttt agtattgaaa cggtggttga cgacacacct ggcgaagtcg cgttcgtgca
      421 cactcctgtt tcgatcgcaa tgtataacga tgaaatacag gttcgcgtta ataataatat
      481 cgtgccactt aaagaaggta attcacccta caccaccgtt tgtgaagcca ttcaatatta
      541 cgttctctct gaaattgata atctcaagcc tgacggcacc cagaaaatgg ttcaactctg
      601 gtaaaaagga cagccccatc acggggctgt tttttcatca agaagagcat aagagttaaa
      661 acggatcacc tcttcgccaa gccagtcatt gatgtgcttc atagcctcca taacgggcat
      721 cagctcgtta attgcgtaaa cccgcgctgc cttctccaca tcgccaaacg cacttttttc
      781 gcccggcatc gcccccatca gttgcggcgg aacgcggtgc gcagccagca catcatcacg
      841 ggatgccgcc ttaacattca tgaattcatc ttttgcggtg atctgctgga acggcaaaat
      901 ttgcaccccc tctttgcccc cgttgggcgc atgaatgagc acgtttttaa acgcaccacc
      961 accacgtgcc ccctgtagcg tttctttcag ggagtccatg ctttcgcggt ttacctgcgc
     1021 tgcaccgatg tagatgatgc acccggcgtg ggatccgttg tcgtagtaca gttttctgaa
     1081 catgtccgcc gaatgagaaa ggctggccga gagtaatgcg ccaagatatt ccggcatgcc
     1141 gtagatttcc tggttaatat ccggattcat caggtggcac actttgccag gacgaaactg
     1201 aaacgcgtcc ttgccatcct gcacatacca ccatgattca agatcgcttc cgcgtcgcat
     1261 gtatttcgcc agggcgtgcc gtaatttaag cggttcgccg agcatattgc ttcgaagctc
     1321 aaggaatgcg ttaccgaaca caaaccagtc cagcgccagc gccgagaaat cctgccggga
     1381 aagcagcggg tgcgggatgt agcaaccgag taatacattg cgcttaaagt aaagcgcaga
     1441 ctgatgccag gacgtttgcc gggctgctct tgccagaccg taccagtcca ccggggtttc
     1501 ataccaccgc ccgttatcag cacagtacat attgtccagc aggtcatgcc cggtcaggcg
     1561 ataaggacca tcaaatgtga atgcactgag cgatgattct ttcctgagcg catcagcgag
     1621 atcaatgcgt gaactcatgc gcactttttt attttttctg ctcatcagaa ctccataacc
     1681 atgaaacgct cgttttctcc ttcgccgcca attggttcgt taatgacagc aagcatggtt
     1741 gcccacgcaa ggtcgccgtg gctgatcccc ctcgcgcggt ccgtttcgta agtgataaag
     1801 ccgcccggtg ttttcacctt acgcacggcg ttaaaggccg cgaccagctc gcgttcggcg
     1861 cgatcgtatt cccaccgccc ggcacgcatt atttgcagca ttttcagtac cagcgaccgt
     1921 tttgatgaca gcgtgaaggt gtacggaata gcagcaggga aaaaccgctt cactatctga
     1981 taaacagcct ccccgttccc gcccgtcaca tcaatgccga tgtgttccac gttgtagcga
     2041 cacgtgaact cttcaatgac tctggcctgt tcttcaaact ccagcccctg aacgcgtcgc
     2101 gtctccaccg ttcgaaaacg gccgccagga acagccggag gaaccaccac ggacacagcg
     2161 ccgctgtcgc cgttgccact actgccgttt gcgtcatacc caatccatac cggacgattc
     2221 cccatcgggc ggggagcaaa aggtttccag tctttccagt cgtcgtatcc gtcaacaccg
     2281 cagccaatca ggatattcag gttaaatgcc gattcccctt cgcggacaaa ctcacacata
     2341 tagagattga ggaactcgtc ttcggtgttt tcatcacgaa tttcgtcgat atcggtgtgt
     2401 ttccagccgt gattaaccac atcttccagc gtgacaattt gccgccacgt ccggtcaggg
     2461 cagataagcc cattatgcag cgttttccag tccacagaaa aacgctggcg tttatgcgag
     2521 gcctttttct cgttccagcg gtcgccgttc cagtaggcgt atgcctcgtg cgtttcggtg
     2581 gatggcgtgg agaagtaggt gcgccgcagt ccgctgaggg ttgccatagc gccagccacc
     2641 ttgcgcagtt cagcaaagcg actgacccag aaaaattcat caaaataaaa attgcccgta
     2701 taggactgtg ccgacgcagc agaagtgccg agaaaatgca gctctgcgcc gttggagagg
     2761 atgattttat cgcccccttt cagctccaca tcaacttcag ccgcggcctt ctgaataatg
     2821 cttttaaact ggaacgcctg acgacgcgac gcagacaaaa aaatctggtt acgctggtaa
     2881 ggttgcgcca catcgtcacg cagcgccatc agcagtgctt cctgtgcaaa ataccaggtc
     2941 gccccaatct gtcgggattt caggatcatc ctgttacgta tcccggcttc cctgcaaagg
     3001 gtcagggagt caaaccagcc ccgctgatgc cactccagcc tgctgatgat tttttcccgc
     3061 agtgcggcaa tctgttccgg cgtgaaatga tttttgagtt ttttcgcccg gcctttcttt
     3121 cctgcggcca tcacatccgg ctggccatca tgcagctttt taagctgccg ggtcagcagg
     3181 tctatttcct taaagtcacc gcctgtttta ttctgttttt cagtaagctg gatgaggcgc
     3241 gcatcgatgg actgcgtgac acgctgcacg ggtggcgttt catcccactg gtcacgtttt
     3301 ttccacgcat aaatcgtgtt cgggtttatt cccatcagac gtgatatttc tgcgggcgga
     3361 taaccctgcc agtaaagttg ccgcgcacgc tggcgcacaa aagcgtcctg aatcattgct
     3421 ccccctgagt aattacagga agattacccg cgcgcgaaac tgttctcctt aaccctctgt
     3481 tctgactgtt ttcttacaac aaaagccctt tgtatcagcc tgttacgctt tgccatcatg
     3541 actgaagaac cagtcagagg ggcaaaaact atggctaatg aaaaaaagac atcccgcaaa
     3601 aagtttcgcg tggctgtctc cggatcaact gttgatggcc gtgaaatcag tccggtgcat
     3661 ctgcgtgaag ccgccgagaa cttcaacccg gatgtttacg ctgcccgcgt gaacgttgag
     3721 cactatctct cgccatgtcc gtcaagcgaa ttttccgcaa tgggcgatgt caccgcactg
     3781 agtacggaag acattacgga aggtccgctg gccggacgta ctgcgctgta tgcagaaatc
     3841 gaaccgaccg agcgcatgaa gcagcttgtc gctgacggca agaaaatcta ttccagtatc
     3901 gaactgcacc cgcagttctc cgttaacggg cgcgcctatc tggtcgggct ggcgatgacc
     3961 gacaccccgg caagcctggg cactgagcgc ctgaaattca cggcacagca acgtcaggcg
     4021 gtgatgacgt tcaacagtat ccagggtgaa gcaccgctta tctccgaagc catcgagtct
     4081 gaaatcatcg aaatggcaga acaacgccag gaagaaggca cccagtggtt taaccgcgta
     4141 atggggatta ttggtcgtgg ccgcaaagcg gatgacgcca gtttctcccg aattcaggaa
     4201 gcggtggaag gcgttgcaac gtcacaggcc gacattatcg accgttttaa tgtgctggaa
     4261 acccgccatc agcaggaccg ccagaaaatc acgtcactga ccacagagct gacagcattg
     4321 aaggaaaaac tgcgcacgca ggacggcgat ccgcagaacc gcttcaccgc aacgggcgca
     4381 gcctccgacc agctggctga cttctgataa gacaaaggag caaatttttt atgaatctgg
     4441 tgatgtcaga tattacccgc aacaagctgg gttgctatat ggcgcagcag gcgtcgctta
     4501 acaatatccc ggtatctgca ctggtatcgc gatttaccgt ggaacccgcg gtgcagcagc
     4561 gttttgaaaa cgcctcaaag gaaagtaccg aatttacgaa aagaattaac gtgatcggcg
     4621 tgaccgacca gaaaggcgaa aaaatcctcc tggacaccac cgggccaatt gcgcgcacga
     4681 atagcagtta tgacggcatc aaacgccgta acccgaataa cgtgatcgat atgaagtctc
     4741 gtcagtacca gtgcgaacag gtgaactacg atacctttat ttcgtaccca cagcttgata
     4801 cctgggcggc ccacagcgat tttcagtccc gtatcagtac acagatcgcc cggcaggtag
     4861 cgcttgatcg catcatgatt ggctttaacg gcacatccca cgcctacgag tctgattttc
     4921 acaccaacaa gctgcttcag gacgttaacg tgggctggct ggagcacatc agaaccgatg
     4981 ccagcgagcg cgtaatgaat gacgtgacgc tgacctcccg caacatggac aacactgtgg
     5041 cgcacgcggg taagtatgcg aatgccgatg ctctggtaca ggatgcgcgc tcatccctgc
     5101 tggatgaatg gcacaaggaa gctgacgacc tcgcggtgat tatggggcgc aacctgttta
     5161 actcgctgcg tctgcccgtg ctgaacagca tcagcggcca gaatcccaat gcggaattac
     5221 ttgccgggca gctcatcctg tcatcgcgca ccattggcgg gctgggcgtg ttccttgcgc
     5281 cgttcttccc ggatgcaacg atgcttatca cctcgttcaa caacctgtcg atttactggc
     5341 agaaaggttc aatgcgtcgc ctgatgaaag acgagccgga atacaaccgc atcgccacct
     5401 accagtccat caatgacgct tatgtcgttg aagactatgg caagtgcgcg atggtcactg
     5461 gcctgaagtt cgccgacagc taatcaactc acggcgggca tcatgcccgc ctgtaacgga
     5521 gagaaaaaat gattactcct gcacaacaac actggcagaa cgtgatggca cagcgcgcag
     5581 gccgggcgaa tgaaggtgtg gaccacgccg cgtgtaccgc gcatgaagag gtgctgtatc
     5641 gtctgcgtct ggcacaggcc aggctaaagg ccatacaggc cagaagcgcg aaagccgcca
     5701 tcaaaaaaga gttgttgccg gacttttccg gctggattga gggaacgctg gaggctgacg
     5761 gcgggcaaca ggacgaagta attgccacgc tgatggtgtg ggcgattgac tgcggcgatc
     5821 ttccgctggc gctgcgtatt ggtgcatatg tggtccgtca caacctcatc atgccggata
     5881 actttggccg tactgctgcc acggtactga ccgaagaaat ctgcaacccg gtactgacgc
     5941 aggctgggac ggatgccgac gcggatttat ccgcctttat cgaaccactg gacacacttt
     6001 gggaaattat cgccaaccag gacatgccgg acgaagtgcg cgccaaatta tgcaaggcgt
     6061 gtgcctttgc ccgtcgtggc ctgaccgatg cagacaacat ggcctcatca ctgaagctgc
     6121 tgcgcgaagc gatgcacctg aacccgaacg caggtgtgaa acgcgagatt gcaacccttt
     6181 cccgcgccct gaaaaaagcc gattccgcag ccgaaccaga agacgccagc gcacagcagg
     6241 cgcaggacga aagcagcaaa agtaaaaaga caacgcggaa gcctgcaaca cgaaaaacca
     6301 ccgcgacgca gaaggcaaag cgcggttaac gactgacccc gtcagcgggc ggcgtgcgcg
     6361 gtgttccggt ttgactccgt gaccgtttac accgcgcacc caccgcccga ttttttcagg
     6421 agtgaacccc atgagtatgg ttgccagaac taaccccggc cccgcagagg acgacatcac
     6481 cgacaccgat gatggcgata cccgtatttc agcgggtgca ttctggccgg atattgtgct
     6541 gcgcgagctg cgtctggcgg tacgactgcc gggccgcgtg accacctccc gcctgctgca
     6601 taccgccacc ggggccatag cacacgttac ccgcgagctg gaagcatggc agcaggaaca
     6661 acaggcggct ggctatcaga cgctggccga tgttccggcc cctgtaatta acggagaaag
     6721 cgtcaatctc tggcactggc gcaatgctgt ttataccgcc acacgcgccc tgattctgga
     6781 gcgttaccgc gatgcggaca caacggacaa gggcgaccgc cgggcggacg cactggatat
     6841 acagacatcg gatttgtggc gcgatgtgag ctgggccatc tctgacattc tgggacgacc
     6901 gcgaatgttt gcggagctgt gctgatgaaa gtgaaggcac tggaaggcga caccgtggat
     6961 tcgctctgtt tccggtacta cggcacgaca cagggcgtca ccgaaaaggt gctggatgcc
     7021 aaccccggac tctgtcagca ggtatttctg gacgccgggc aggaagtgga gatgccggag
     7081 ccggagaaga agaaacgaga aatgattcag ttgtgggggg agtagcagtg agcaccattc
     7141 aaacagggat cacagagcag gttattgcgt ggctctttga ccacctgcca acggtgtatg
     7201 cagtaggcgc ggcggtcagc atttccgcgc tgatgagtct ttatgacgga cgaacactgg
     7261 ttcagaccgt aacgggatcg ctggcgtgcg gcgttcttgc catggccgtg gccgggtcgc
     7321 tgcgcttctt cgggatccct gaggatgcag tgacgttttt tggtgcctca atcggtttta
     7381 tgggcgcaga gaaagcacgc gacaaggtta ttgcaatatt tgatcgcagg gtgaaggaga
     7441 ggaacgaatg agcaacacat ttaaattcag cagccggagc gaaaagaatt tgcagggtgt
     7501 aaatcctgat ctggtgaaag tgacccgacg ggcgctggaa atctcggaag tggattttgg
     7561 tatcaccgaa ggattgcgca gtcgttatcg tcagaagcag ctcgtgacca caggcaagag
     7621 ccagaccatg aacagtcgcc atctcacagg gcatgccgtg gatgttgtgg cttatgtcgg
     7681 caaccaggtg tcatgggaat ggccgctgta cgaaaaaatc gcagcagcat tcagacaggc
     7741 cagccaggaa ctgaatattc cggtggaatg gggcggcgac tggaagaccc tgaaagacgg
     7801 accgcatttt cagttaccac acggagccta tccggcatga agctctggcc cacgcttggc
     7861 gtcgctttcc ttctgattac atccatgcgt ctgtcgtggt cgctgggccg ggagaacgcc
     7921 agaaacgaag cgcaggccag caccctgaaa agtaccgtcg acacactgaa tatcatcagc
     7981 gccggggtac aggatatgca gcaggtgctg gctcagctcc gcgcggaaaa tcagcaacgc
     8041 aatcaggacg gagaggtaag acgtgaacag ctacgcaacg atattgcaaa agatgaatgc
     8101 gcccacgctt tgcctgacgc tcgttttact gacaggttgc gcaggcacgc agaacgcgcc
     8161 agggccagcg ccgtcagtcc ggcttatacc gcagacgctg accatgccgg taacgcctcc
     8221 ccccttccct gacccaccca catggggaaa cctcggaata tggggcgacc gccttctgga
     8281 tgcactggaa acctgtaacg cggataaacg ggccattgct gaactggata agagaatagc
     8341 cgaactgaca caccagacgg gagtaacaca atgaccagta agaactttgc actgattaca
     8401 gccatgacac aggctgaact gactcaaaag gtgaatgaac atcttgcgaa agggtggcat
     8461 cttcaggggg agacgcgggt tgcctacgaa cccggcaccc cgtggtatct aatgcaggca
     8521 atggtggccg atggcactac agacatctca cctgattccc cccagcacgg cagcgtgccg
     8581 gagtggtatt acgtggtggt acttgctggt caatccaatg ccatgtcata tggtgaggga
     8641 atgccgctgc cggattctta cgatgcgccc cacccacgca ttaagcaact ggcccgtcgc
     8701 aacacagtga ctcccggtgg taaagcatgc gcatttaacg acatcattcc ggcagaccac
     8761 tgcctgcatg atgttcagga tatgagcgca ctgaatcacc cgaatgcaga cctgagcaaa
     8821 gggcagtatg gctgtgtcgg acagggcttg catattgcca aacgcctgtt gccttacatt
     8881 ccacagaatg ccgggatttt actggttcca tgctgtcgtg gtggttcggc attcacccag
     8941 ggcgcggagg ggacattcag cgagtccaca ggagccagtc aggattcggc tcgctggggt
     9001 gtgggtaaac ctttatatca ggacctgatt ttgcgcacga aggccgcatt gcagaaaaac
     9061 ccaaaaaaca tgctgctggc cgtatgctgg atgcagggcg aatttgacat gagcgccgct
     9121 acgtactcac agcaacctcc gctgtttacg gccatgctga aacagtttcg tgcggacatt
     9181 accgagttta acacgcagtg tcatggaggc agagcggcaa gtgtgccatg gatttgtggt
     9241 gacacgacgt attactggaa aaacacctac ggcacgcagt acgacaccat ttacggggcg
     9301 tacaaaaaca gggagagcga caacgttttc tttgtgccgt tcctgaccga tggtagtggc
     9361 aacaatacct ccaccaacgc accaacggaa gatccggatg ctgcaagtga gggatattac
     9421 ggttcggcat cccgaacgaa caaaaactgg gtatcatcaa atcgccagac gcatttcagc
     9481 tcatgggcgc gtcgtggcat tattcccgat cgtatggcaa ccgctattct gaacgtagcc
     9541 ggtcgcacct tagccttcat cagtggtaag gcaccggaaa tcaaaccctc gcccggcggc
     9601 gacacgccat cggggccgtc tgaagatgca tccgtacgca caatctccct gttgccgaca
     9661 gccggagatg ctgctgcgca gggctggagc attaagaatg gcggaattca gttgtcagag
     9721 ggtgtattta agatcaccaa gcagagcaat aaagcctggt ccctgacgcg cccggtggat
     9781 gacgcagtct ccctgctgac acggggtggc agactgagct gtaagtttcg actgtcaggc
     9841 gcactgacca acaatcagtt cggtctggga atttatctgt ataccgatgt agcgttacct
     9901 gacgtcgtgg cgatgaccgg gactggtaac ccgttcctga tgtcgttctt cacccagacc
     9961 acagacggca aactgaatct gatgcatcac aagaaagcag gaaacacaaa gttgggcgag
    10021 ttcgggaatt acagtaacga ctggcagacg ctggagctgg tgttcaccgc cggcagtgcc
    10081 acggttactc cgaaactgaa tggagtggct ggcccggcat tccaggtcat aaaagacagt
    10141 ctgacactgg ggctgaatgc gctgacgtta actgatatca ctaaaaatgc cgcgtacggc
    10201 gtcgatatcg gcagcctggt gctggaaatc aataatcccg cagcataagg aagagcagga
    10261 gagcaaaaca gatgcttaag acaaacagtc tgcgagagtc catgcttcat gggtgtcggt
    10321 ggtgccaggc taatcccgag aaattcacca ttttcgtgga gagcggcaac attgaaacga
    10381 ccggagaagc gccctcgttt gtttaccgct atcagatggt gatgtttgtc atggattacg
    10441 ccggagagct ggacgacctc acgctgccgc tgctggcgtg gttatccgaa aatcagccac
    10501 agttgttgct caatccggag cgtaatcagg acatcaaatt ttctgccgtt atcaatgacg
    10561 atgacagcgc cgatctcctg tttacgctcc ccctgcggga acgcgttcgc atcacgcgca
    10621 acagtcaggg cacaccgcag gcagaacacc tgccggagcc aaaaccccgt ctgccttctt
    10681 ccgaaggcga ctggtcgcat gtattccagg atgtgacgtg gggtgaaagc gatggataag
    10741 gcattcaccc gcgtggatga aacctttgag gctatccgcg acagcctgaa tcagcaggcc
    10801 atcaataaca tcgccagaaa gctggcacag gatttacgtc gcgcccagca ggcacgtatc
    10861 cggtcacaaa aagcgccgga cgggaccgaa tggacaccac gcagacgccg cgtaacccgg
    10921 atacaggagc gcattcgctt tatctggaat aacgaagcac gcacgctgaa aaactggcat
    10981 cacgacacag ggaaatacgg gcgaaccatt accgggtggg atgaggataa aaacaatatc
    11041 cgcacgtttt accgggatga catcgaccgt tttctggaaa tacgcacccg gcgcatcaac
    11101 caggacagca caaagcgcgt ccccatgttc gtaaaactgc gcaccgcccg ctacctgaaa
    11161 gcccgtgcag atgcttccgg tgtgacggtg ggttacagcg gcgtggccgc acgtattgcc
    11221 cgcgttcatc agttcggtga gcgcgatcag gttgcgccgg gcattttcac cgattacccg
    11281 gtacgtgagc tgttgggcat cagtcaggca gatgaacgcc tgatttataa cacggtgctg
    11341 ggccggattg cggaggctgt acggtgagcg cagaactcat gcgactgctg agcaacatca
    11401 tccgcactgg gatcatctct gaagttgatg agaagtcctg gtgcgtgcgc gttcgcagcg
    11461 gcgaactgga aacaggctgg ttgcgctgga acaccacgcg cgcgggagcc ttcaatgtgt
    11521 ggctgccgcc atcacccggc gaacaggtgg taattgcctg cattggcggc aacccggaaa
    11581 ccgccatgat aattggcagc ctgtggagtg atgccaatcc ggcccccggc aaaagcctga
    11641 aagaaatcgt ggtcagcgcg ccggatggcg cggtgttccg ctacgacgcg gacgcaggcg
    11701 cactgagcgc cagcggcatg aaaacggcca ccctgcaggc atccgtcagt gtgacactgg
    11761 acacgcccgt cgtggaatgc acagaccttc tgagaacggc gacgcttgac gtcacaaaag
    11821 ggggaaagat gagcggcaat atcacgcaca gcggcggcaa tttcacctca aacggcatca
    11881 cagtgcatac gcataaacac ggtggcgtta aaggtggcag cgattcgaca ggaggcccgc
    11941 agtgacaacc cgctacacag gaatgaaccc ggacggaacg ggaaacctga acgatatgga
    12001 gcatctgaaa cagtcagtca gggacatcct gaccaccccg ctggcaagcc gggttatgcg
    12061 acgggaatat ggcagccttg tgcccgattt aattgacgaa cccatgaata acaccactcg
    12121 tctgcaatgc atgagtgctg ccgtgattgc gctgacacga tgggaacccc gcattgccct
    12181 ggacgccatc gacgttgtct ggaaggcagg aggccgcgcc ggggtgacgc tgtcgggcac
    12241 tgtcatgcag accatgcaga atgttgaatt aaccatcacg ctgagggagt aaatcatgcc
    12301 tgccgttgac ctttcccagt taccggaacc cgccatcatc gcggagcctg actttgaggc
    12361 aattctggct gacacaaagg ccatgatgat tgcgtcctat cctgccgaac agcgtgaagc
    12421 cgtctccgcc gcgctggagc tggaatcgga acccctgaac gttatcgccc agacaacagc
    12481 gtttcgtgaa atgctgttac gccagcgggt caatgagggg gcacgcgcct gcatgttaag
    12541 ccacagcgcc gggacagacc tggacaacct cgcgggcaat atgaacacaa agcgcctgac
    12601 catcactccg gcaacggata ccaccgacgc agtgatggaa agtgacacct cgctgagact
    12661 gcgggcgcag cgggcgtacg atggcctgag tgttgctggc ccgtcaggtg catacgagta
    12721 ttttgcccgc agcgccagcg gtctggtgcg tgatgcgcgg gctatcagtc cgtctccggc
    12781 aaatgtgacg gtttccatcc tgtccactga aggcgacggc acagcaacgg aggcgttgct
    12841 taataccgtt cgcgccgttc tgaatgcaga ggatacccgc ccggtggccg accgcctgac
    12901 ggtacagagc gccagaatcg tgacatggcg gctgaatgca aaactgtact tttaccccgg
    12961 cccggaatcc gaacctattc tggcggcggc tgaatcgtcg ttcaggaagt ggctggctga
    13021 gcaggggctt atcggtcagg acgtggcgtt gtccgccatt gctgccgcac tgcatgtgca
    13081 cggtgtgcaa cgcgtggaga taatcgaacc cacacagaat atggccatca gcgacataca
    13141 ggcggcgcgc tgtgagtcgt tcaccatcag cgaaggtggg cgcaatgagt aattcactgt
    13201 taccaccatc agccagcagt ttcatgcgtt gtgccgaagc tgtcggaacg cgcattacag
    13261 acatcccggt agacctcaac acgctgtggt cgccggacac ctgcccggtg cacctgctgc
    13321 cttatctcgc ctgggcattt tccgttgacc gctgggatcg caactggccg gaagagacaa
    13381 aacgacaggt gattcgtgat gcatggctga tacaccgaca caaagggacc atcagcgcac
    13441 tgcgcagggc cattgagccg ctgggatacc tcattcgcgt gtctgagtgg tgggagttcg
    13501 gcggagaacc gggaacattt accgttgaag tcggcacact ggacagtggc gtgacggagg
    13561 aaatgtatct ggaaatggag cggttgattg ctgatgcccg tccggtcagc cgccacatga
    13621 cagggctgaa tatcattcag gaaattccgg gggatatttt tgcagcggcg gcaactcatg
    13681 acggtgaagt tattaccatt tatccggacg attaagcatg agtaccacaa cacgaaaatt
    13741 taaaaccgtt atcaccgata caggtgccaa aaaattagct caggcagccg cgccagatgg
    13801 taagcctgtc cgcctgactc atatggccgt gggcgacggt ggcggcgcgt tgcccacacc
    13861 agacagtaag cagacccgtc tggtgcatga ggtgtggcga cacactgtta atcgcgtcat
    13921 cctggacgca acacatcaga accgcattat tgcggagctg gttattcctc cagaaacggg
    13981 cggattctgg atccgggaaa ttggtgtgtt tgatgagcac ggcgatttaa tcgcggtggg
    14041 caatactgcc gaaagttaca agccagccgt tgccgaaggg tccggacgtg cacaaacatt
    14101 tcgcaccatt ctgaccgtat ccagcactgc cactgtggcg cttaccgtgg ataacaccat
    14161 ggtgatggcc acagtggatt acgtggataa cgaactgaaa gagcatgaac agtcacgatg
    14221 tcacccggat gcctcgctga ccgcaaaagg ctttgttcaa ctcagtagcg ccactaacag
    14281 cgattctgaa acgctggctg caacgccgaa agcggttaag gtcgcgtatg atcttgctaa
    14341 cggaaaatat acagcgcagg atgccacaac agcgcgaaaa ggccttgtcc agcttagtag
    14401 tgcaaccaac agtacatctg aaacgctggc ggcaacatca aatgcagtaa aagctgccta
    14461 tgacaatgct gaaaaacgtc tgcagaaagc taagaatggt gaggatatct ctgataaaga
    14521 cacctttacg aaaaatatcg gtgcctgccg tgcatatagt gcagagctga atattggtgg
    14581 agatagtgaa gcatggacaa ctgcgcagtt gattttttgg ctagagagtc agggggcatt
    14641 taaccatcct tactggatgt gcaaaggctc atgggcttat gcaaataata aggtcattac
    14701 agatacaggt tgcggaagta tttgtcttgc aggtgctgtt gtggaagtta ttggcacccg
    14761 cggcgcaatg accatacgca ttaccacacc gagtacatcc agcggtgaag gcatccctaa
    14821 tgctcaattt acttatatta atcatggtga tgcttatgct cctggctggc gaagggacta
    14881 taactccagg aataagccaa cagcatcaga gatcggggcg ttaccgtcag atgggacagc
    14941 agtatcgtca gttaatctgg cttcaaaagg tcggctgacc gccctgacag ataatatgca
    15001 gggggccaca ggtctggagt tatacgaggc gtataacaac ggatatccaa caacgtatgg
    15061 aaatatcatt cacctgaaag ggatgacagc cgttggcgaa ggcgaattac tcatcggctg
    15121 gagtggtata agcggtgctc atgctccggc atttattcgt tcacgacggg atacgcccga
    15181 cgcaaactgg tcgccgtggg cgcagcttta cacctcggct catcctcctg aagagtttta
    15241 tccagtcggt gcaccgattc cgtggccatc agataccgtt ccgtctggtt atgccctgat
    15301 gcaggggcag acttttgaca aatctgcata cccgaaactt gcagtcgctt atccgtcagg
    15361 cgttattcct gatatgcgtg gctggacgat taagggcaag cccggcagtg gtcgtgccgt
    15421 attgtctcag gaacaggacg gcattaaatc gcacacccac agcgccagcg catccagtac
    15481 ggatttgggg acgaaaacca catcgtcgtt tgattacggt actaaaacga ccagttcatt
    15541 tgattacggc acaaaaacta cgaatagcgc tggaaatcat tcacacaata tacctgttgg
    15601 tcacactggc gcggggaatg gtgtatcagc cggttataac gctgcgttag gtactggtac
    15661 cacgtcgagc gcaggcgagc atgctcacaa tgtatatatc ggtgcccata accacactat
    15721 cggcattggt gctcatgccc attctgtcat tattggtccc cacggacaca ccatcaccgt
    15781 taacgctacg ggtaacgaag aaaacaccgt aaaaaacatc gcatttaact atattgtgag
    15841 gcttgcataa tgacattcag aatgagtgaa cactcacgga ccataaaaat ttataatcta
    15901 ctgaccggaa ccaatgagtt tattggtgaa ggtgatgcat acattccacc tcatacaggt
    15961 ctgcctgcaa acagtaccga tattgccccg ccagatattc cggctggctt cgtggccgtt
    16021 ttcaacagtg atgaggcatc gtggcatctc gttgaagacc atcggggtta taccgtctat
    16081 gacgtggctt ccggcgacgc gttatttatt tctgaacttg gcccattacc ggaaaatgtc
    16141 acctggttat ccccggaagg ggagtttcag aagtggaacg gtacatcctg ggtgaaagat
    16201 gcagaagcag aaaaactgtt tcggagccag gaggcggaag aaacaaaaaa cagcctgatg
    16261 caggtagcta gcgagcatat tgcgccactt caggatgctg tagatttgga tattgcgacg
    16321 gaagaagagg catcgttact ggctgcatgg aagacatatc gggtattgtt gaatcgtgtt
    16381 gatacaacag tagcagcgga tattgagtgg ccagtcgccc cacaataaaa agaaaaagcc
    16441 atcgatagaa atatcgatgg ctttatgtgc tctatttata caatacaaca ccgctctttt
    16501 tagttatata tgtgcagttc gatggtatat ctttatttat aaaagacatt gcgcctattt
    16561 ttacattatc cccgatttta cgtgataatc caatgatgca acaattagct ccgatatcaa
    16621 cgttactacc aatttttact cttgaaccag gtatgtcacc atctatctgt ccaatcgtag
    16681 tattctgtcg taataccaga ttttcacctg catcaacagc aaaatgaaca acaattccag
    16741 catgatgggg aattgttaac ccttttccaa tatttgctcc caatc
//
LOCUS       DAZGCS010000106        16271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGCS010000106 DAZGCS010000000
VERSION     DAZGCS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.106"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..235
                     /locus_tag="ACHK01_003825"
                     /pseudo
     CDS             1..235
                     /locus_tag="ACHK01_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(316..474)
                     /locus_tag="ACHK01_003826"
     CDS             complement(316..474)
                     /locus_tag="ACHK01_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO8098702.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(560..1303)
                     /locus_tag="ACHK01_003827"
     CDS             complement(560..1303)
                     /locus_tag="ACHK01_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098703.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1488..2177)
                     /locus_tag="ACHK01_003828"
     CDS             complement(1488..2177)
                     /locus_tag="ACHK01_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO8098704.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(2192..2314)
                     /locus_tag="ACHK01_003829"
     CDS             complement(2192..2314)
                     /locus_tag="ACHK01_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO8098705.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            2653..3612
                     /locus_tag="ACHK01_003830"
     CDS             2653..3612
                     /locus_tag="ACHK01_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO8098706.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(3756..3827)
                     /locus_tag="ACHK01_003831"
                     /pseudo
     CDS             complement(3756..3827)
                     /locus_tag="ACHK01_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(3824..4489)
                     /locus_tag="ACHK01_003832"
     CDS             complement(3824..4489)
                     /locus_tag="ACHK01_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO8098707.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(4486..5091)
                     /locus_tag="ACHK01_003833"
     CDS             complement(4486..5091)
                     /locus_tag="ACHK01_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO8098708.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQE
                     AVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(5091..5813)
                     /locus_tag="ACHK01_003834"
     CDS             complement(5091..5813)
                     /locus_tag="ACHK01_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO8098709.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
     gene            complement(5888..6622)
                     /locus_tag="ACHK01_003835"
     CDS             complement(5888..6622)
                     /locus_tag="ACHK01_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO8098710.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVLEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            complement(6897..7079)
                     /locus_tag="ACHK01_003836"
     CDS             complement(6897..7079)
                     /locus_tag="ACHK01_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO8098711.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(7076..7603)
                     /locus_tag="ACHK01_003837"
     CDS             complement(7076..7603)
                     /locus_tag="ACHK01_003837"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO8098712.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(7600..8046)
                     /locus_tag="ACHK01_003838"
     CDS             complement(7600..8046)
                     /locus_tag="ACHK01_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO8098713.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(8003..8239)
                     /locus_tag="ACHK01_003839"
     CDS             complement(8003..8239)
                     /locus_tag="ACHK01_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO8098714.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            complement(8250..8465)
                     /locus_tag="ACHK01_003840"
     CDS             complement(8250..8465)
                     /locus_tag="ACHK01_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098715.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(8598..8876)
                     /locus_tag="ACHK01_003841"
     CDS             complement(8598..8876)
                     /locus_tag="ACHK01_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098716.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            complement(8947..9237)
                     /locus_tag="ACHK01_003842"
     CDS             complement(8947..9237)
                     /locus_tag="ACHK01_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO8098717.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(9234..9935)
                     /locus_tag="ACHK01_003843"
     CDS             complement(9234..9935)
                     /locus_tag="ACHK01_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8098718.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(9932..10870)
                     /locus_tag="ACHK01_003844"
     CDS             complement(9932..10870)
                     /locus_tag="ACHK01_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8098719.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(10903..11199)
                     /locus_tag="ACHK01_003845"
     CDS             complement(10903..11199)
                     /locus_tag="ACHK01_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO8098720.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            complement(11338..11571)
                     /locus_tag="ACHK01_003846"
     CDS             complement(11338..11571)
                     /locus_tag="ACHK01_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8098721.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            11685..12389
                     /locus_tag="ACHK01_003847"
     CDS             11685..12389
                     /locus_tag="ACHK01_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8098722.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            12450..12791
                     /locus_tag="ACHK01_003848"
     CDS             12450..12791
                     /locus_tag="ACHK01_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HFO8098723.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRANPDCCFFG"
     gene            12859..13320
                     /locus_tag="ACHK01_003849"
     CDS             12859..13320
                     /locus_tag="ACHK01_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098724.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            13314..14360
                     /locus_tag="ACHK01_003850"
     CDS             13314..14360
                     /locus_tag="ACHK01_003850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HFO8098725.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIAMPFYHNGSLNQLMKKN
                     NLTSREIIRYSIQFLSGLYHIHSKGLMHFDIKPNNIMISNRNEAMLSDFGLSQLVNEE
                     SRAAPEFGYHFHVPPEYFSLSTNDYNFTYDIYQAGLTIYRMCVGHDNFERERSAFSTI
                     EQLRESIINGCYPLKEYPPHIHKKLITIVNKCIHVDPNERYQSVLDVLNDLSAISDGV
                     LDWRLQMTKPTNGTCEWQKKSGDAILSIVFDAENSSTTGFRLYDDGRKRRATNLTISS
                     GCTPTKLYRLLKDN"
     gene            14363..14527
                     /locus_tag="ACHK01_003851"
     CDS             14363..14527
                     /locus_tag="ACHK01_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098726.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
     gene            15341..15409
                     /locus_tag="ACHK01_003852"
                     /pseudo
     CDS             15341..15409
                     /locus_tag="ACHK01_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            15471..16094
                     /locus_tag="ACHK01_003853"
     CDS             15471..16094
                     /locus_tag="ACHK01_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO8098727.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDRLSSKLVAISEELGKMGCSFYSYDVSSTSIYLTVSGRRVGWHSYG
                     KDGNGKDILLPTPIKDKCMFDAEHEITKRFDEICALQQKLEAKKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
BASE COUNT         4573 a         3754 c         3422 g         4522 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggcaggt cataaaaaaa aggctgcgca
      121 atgcgcagcc agaactcaca aggaaaatga taaaaggaat aacactagtg atgtacgcat
      181 ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacgtgcc cttaaaactc
      241 gatcatttag cccctccaag gaggattcac catgcggttg attttttaat aaacagtaaa
      301 caaaaaagtc aagaattatt cattctgttc tttcatcatc ggccacagca ataccacaat
      361 gccgcagacc agagcgccat cagtcagtac caacattatc ctgctggtga aatccatcat
      421 caccatcact aaaagcagga tcacaacagc aagcagacac agtttataaa acaatgttca
      481 gaaaacgcat tcagcatgcc taaggttcta ttcctacgaa tagccaactt gcaacttaaa
      541 atattattta tgcagccaat taaattctgg tccttacaat atcaacctga agattcttat
      601 cttgtgctga ttgataaatg acaaaccttt tactacctgc attgaaagaa gtagacaaaa
      661 ccagacaatt atcataacga gcaagaacat aataccaacc atcattataa ttaatcattt
      721 catattcttt cttaaactgt ggtttgtaat atcctgtcag aaatgaaaaa agccaaaaat
      781 atgccacaaa agcaatcatc acaatctcaa aaaaatgttt ttttataaat ggcttatcat
      841 agaagcatga taccgataaa aatcgcccat aagatcttat cgaaattgta accgccagcg
      901 caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat atagaaaact
      961 caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat acatttagct
     1021 cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata ccccaaccga
     1081 taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca tcatcaacac
     1141 ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat ccatagaaat
     1201 aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa ctcctgagca
     1261 gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta caactcataa
     1321 caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt acatagcagc
     1381 cagcatgttt accgtacaag tacaactcag ggcataaaaa aaacccactc ggcagcgggt
     1441 ttatacattt tttacaacat accaaatttg catgaagtat atggctttta atccagtttt
     1501 gcaatatttt gctgtaaaaa tgctgccttt tgttttgaac gtgttctcgt cacaagcaat
     1561 aaagcatcac tatcaagctg tagaaaaatg tgcttcattg caacccagcg ttcagtaaat
     1621 gtctcggacc agttttttgt tgtcactccc accaatgatg ccagtgtctg gtattcatag
     1681 gcctcacgcc ctgcaagttc gctcttcaca tcctgtgcag ccagccagat taacgtcttc
     1741 aggcgatcca gtgtcttacc tgcaattttt ctgttactta acaaatcttt aaactcgctc
     1801 catgcccatt gcgttatggt gacctgatgc ccccatcgaa cgctttcgct gtaacaccaa
     1861 agcaaccatg ctttctgatg ttcatcgaga gacaaaaccg cgcggcgcca tgaagaggtt
     1921 gagaattcaa ccgggctgac caaagcaatg gatgaacctt ttgcgtacga ctgcttaccg
     1981 gaagtcggcg tattatccag cgtaatcatc ttgccagtta ccacatccag aatgcgcggc
     2041 ttctttcgtt tgtatgtacc agtatcaaat tgtgcatgct cctgccaggc ttcgagctgg
     2101 cctttcgttg ctccgttcaa gtcagcagta gctgccataa gttgctcacg aacatactgt
     2161 aaatattggg tattcatgca gtaaatcctt tctatatttt ggcataattc ttcaacattc
     2221 ggtaatcgtt caaaaccgaa tcggggaaac gacataagca caggagcccc cagcgacagc
     2281 gaaggagttc tgatatataa gactcagaca tcattcattc cccggttctc caatatctgt
     2341 ttcactcatc atccataact acctgtaatt gccccccctt tttttgtaac agttcttata
     2401 ttgctatata gaatagccat tactaatgca tttaaattta ataaaataaa aattataaaa
     2461 aacataaaac accacgtaaa cacacttaat aaaaacaccg ttacattaaa agataataaa
     2521 aaccgcaata aaaaaagaat aaatcaattg tctcacgcaa ttataaaaca tcatattgat
     2581 tacgcacctt gtattacaaa ctcatgtatg taaaatacgc gcaccattca aaaaaaagga
     2641 agacaataac atatgaaaaa aagtgtcatc gctggcgtct ttattgctct gtcatttacc
     2701 acgtgttcag ctatcgcgaa cagccttgca ttatcattag caaatgatga tgcagggaag
     2761 tttcaaccaa tacttaatga tatttatggc aataaacatg aaaacagaga tgattactca
     2821 caaggcttat ttctgggata tagccacgat atctcagact cgagccaatt atctctccat
     2881 attgcgcaag atatttactc tccatcaggc agtaataaaa gacacaacac agctgtaact
     2941 ggagacagag cttttagtgc atacactcac actggtattg aatggaactc ccttgcgaat
     3001 gactggattc gctatcgatt aggtactgac ataggtgttg ttggccccga cgcaggcggt
     3061 cagaaagtac aaaataaagc tcatgagatt attggggcag aaaaatatca tgcatgggat
     3121 gatcaaatag agaatcgcta cggttatact gtaaaaggga tgctatccat gacaccaagt
     3181 atggatattt taggtgctaa tgttggatta taccctgaag tttctgctgt tactggaaac
     3241 ttatttcaat atgtagcata tggcgcaacc attgccattg gtaatgataa aaccttcaat
     3301 tcggataatg gctttggtct gctggctccc cgtggtttaa tgcatatgtc cgatacaagc
     3361 ggattcaaat acaagatttt tgcaggtatg gaaagacgag atgtcaatcg caactatact
     3421 ctcgaaggaa aaacaataca gacgaaacaa acaacagtat cgctaaacaa aactgttgat
     3481 gaatatcaag ttggcgcaac aattgggtat gcacctgtag ccttcacact agcatttaat
     3541 aaagtaacat cagaattcaa gacaggggat gactattcat ttataaatgg agcaatcacc
     3601 ttcttttttt aactgaattg aattcaatca aaataacata agtccaacaa aaacataaag
     3661 tgcgaaatga atgccagctc catttatttc gcactataaa agattaaaag ttgcaataaa
     3721 ataataaaat gactcagtta cgaaaaccaa taaactgtgg ccagtagtga gtcgctcatc
     3781 atcgggcttt ttggcgaatg aaatttagct acgctttcga gtctcatgcg ccttctccct
     3841 gtacctgaat caatgtgagg tttccgcaga acactgcgcc agtatcgata tacatctggt
     3901 tggcaaattt gagtggtttc actgctggcg tatgaccaaa gatgaacgtg tccgcgcctt
     3961 tgatttcttt cacgatcccg tcttgtgagt tgctgattcg ttcgcggttc cagattacct
     4021 gctgatgatc aactggcttt ccaaactcgt atttatcaca aggataatcg gcgtggcaaa
     4081 tgacatattt tttatctttg ctcaccagtt cgatgattaa cggaagttct tctgctttat
     4141 gggcaagaac tttagccaga gtctctttgt cgtaatcgag attaaagaac cagccaccgc
     4201 cattaagcag ccagtgatta acgtttccac gctctgataa gccatcaatc atcatttgct
     4261 catggtttcc acgcacagct ctgaaccagg ggaatgtgat taattccaga cattcgacgt
     4321 tctctgtacc gcgatcaacc aaatcgccaa ccgagatgag caggtctttt ttggtgtcga
     4381 atccaatcgt atccagtttg ttcatcaggt tcgtgtagca tccgtgcagg tcgccagcta
     4441 cccaaatatt tcggtatttg ctgccatcaa ttctttcgta gatattcatg cagcctcact
     4501 tctgctgttt cgcaggtctt tgagtttctg ttggtactct gccttgatcg ccttgcactc
     4561 ttcgacagtc cagcgatggc ggttatggtt tgattcgatt tcgtctactg cttcctgccc
     4621 gatgcggcta atcagttcga cgcgatacgg aacgagattt ccgcttttgt gctggttgca
     4681 caccacgcat tgcttgtgaa tattgcgttc atcaaatcgg agttgaggtg ccgcagcagt
     4741 tgtccggtaa tgtccggcat cccactgagc agacgtgagc gttccgcacg agatacatgg
     4801 taagtcgcgg tctctttctc tgatgaaggc gtttacggct tgttgggctt gtttaatcca
     4861 gtaactgcgg ggctttaagg cgagttttcg aatcttcagt ttatctttct gtttctgctc
     4921 ctctcgtcgt cgtttcttct ctgctgcttt ttccgctttt tcgcgttctt tacttcgtcg
     4981 ttcgagtgct aattgagttc cgtgttccgg gcagcaccac cactgatttg agaatgccgg
     5041 gtgaaaccat tccttgcata ttttgcattt ccttcgcgct ggtttagcca ttaagcagcc
     5101 tcccttgtta ctttcagcat tccgttatcg agcagctttc tggtcagcca ctgttgacca
     5161 cgcccggtga tttttgtggt gaacgatatc tgtattccgt gatttgtatt gaccgctgtt
     5221 tctttcactg tgaaatagcc acgatccata tattcctgca ttggcacatt gcgccgggag
     5281 cctgaagcaa taaggatttt gtgatcgcgc atccacgcaa acagtttgtt tggaccaata
     5341 ccaacaacct ttgcaaagtt tccaatcaaa attccgctgg cctcgccaac gcgatcggca
     5401 aactcaactt taggtgctgc gagagcaagc tgtttctcca gttcagcctt ctggtcttca
     5461 aggtcggccg caaggcgcaa tgcctcagaa aaggtttgtg gtattttcgc ggttgcccct
     5521 tcgagttctc gccagcggtc aacaaggcga gcggtgaatt ccggcgacaa ctgggcaacg
     5581 acaataatgc tgtcgcgctt accttgttcg ccctcaaaaa cgtaagcctc tacgccacga
     5641 agtaatccta agttattgat tttttcgaaa accaccattg ggggatttcg gatcacacct
     5701 cgaaccgcca gtcgttcaat ggattgtttc accttgtcat gacggcttcc caccaactca
     5761 gcgatttcaa tgcttgtcat tttgatggca ttgctattta tcagctcatt cattgtcatg
     5821 tcctctcaca ttgaaaattc agcaataaaa aacccagccg aagctgggtt tgttaagttg
     5881 tcaattgtca gtagcgatgc agtgaaggcg gcaactcttt gttcttaagc ctttcccatg
     5941 ccagaaggtt cgtcggcccg tcaggctcat aaatatctat atcccgcgtg tgattaatta
     6001 aaacgcccct cgccctcccg atgatatacg agaactcata gccgtagtcg tggcatatgc
     6061 cggaatagcc agactgaatc agttttaatg cgggatacaa ctcacggaac aatgcctgtg
     6121 agcggttggc ataatcccac agccatacaa ggctgtctgt ttcttttgcg gaaagcccgt
     6181 tgagcttctt ctcttgtttg ccagtatttt tctcgcactg gctgaaatag cagtcttcca
     6241 gtttttctaa cacatcccac gcctgatcag tttcgagcat ttttgcgtga cgggctgcgc
     6301 ctcgttctgt ccagagtatg agggagcggg ctttcgggga aatttgtaac cctcttaaag
     6361 atggttgcaa attttgtgag ttacttaaag taacccgcaa attttgtgag tagtttaaag
     6421 ctacccgcaa ttctttaagg tcattaccaa caactttgaa aaagtgtttc ccttcaacga
     6481 agcgtacttt gttctcatga tgattctggc gaatacgcac cggctcagtg ccgtaaagct
     6541 gcgccaaaag ttcggtggta ataacaggaa tctggttatg ggtgatcggg gagagagttt
     6601 caacagaaat ttgagttgtc ataatgacgc cctctggtgg tttcttaata actcaccacc
     6661 gacgacgcca atcgtctggt ggtgaactgt gcagggttgg cgtaaccggg aaaccgaccg
     6721 gcgcggatct ctccgccccc acacagccca ccataattca gatgtgcgcg tgcatacgac
     6781 aataaaaaac acgctcgcgg cgtgtatctg tcgcggtctc tatccaggac gccaatcccg
     6841 acgccagatt ttgctggcgc gtgaggaata tagccccgaa taaatcatcg cgtcaatcac
     6901 cttgttttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtaattg
     6961 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     7021 ggaaggtatt tgcagttttc gcagatgatg tcggtgatac ttcgtcgctg tcgcctcatg
     7081 ccgccctcct gacgccctgc ccgatcgcca tcaatgccgc tttggataca gtagtaaaca
     7141 ttcgtcgagg actgatgaac ggtcgccaaa tcagcagcat ggagcctttg ctgtttccct
     7201 tcttctccag ccctgtcgat ggttcgataa aattaatccg tccatcagtg atgatgcgaa
     7261 cttcgtcaac actctccaga gccttgctga accatccgac tgacatatcc tctggcacaa
     7321 gcataactac cgtctgtcgc tgttgtatgc actgctcagc ggctttttcc acccacggcc
     7381 tgatattgct gtacggtggg ttattccaga ttgcaccgtg gcttacccac tcagaattga
     7441 gcgcgtcgtc ggcctcagtt agccagtgag cgcacagagc atttttgtcg ctcgctgccg
     7501 aatccagcca gaatccaaac tcaatatcca gtgcatcaaa aagccaaagc ggcgtttgcc
     7561 agcagtcctt gtcgtgtgct ggcgtatttg atttgatagt catgcagccc taccttttcg
     7621 ttgtgaccat tcatactctc gccgggagtc atcactccac cgcacgttgc gctctgagcc
     7681 gaaccagaac atgatttcga taagctcagt catgctggcc ttccgcattt tgctggtacg
     7741 cacgccaagc atgacaacgc caccgtcgat accaggcaca cttcgttgct ccagtttttt
     7801 ggtcttaagc cacagggcag tgaacaggtc tttccagtct tccggcgcca gccgttgacc
     7861 atgccatagc acctgacgcg aaacatcgtt cagcatcggc cacatgcggt cattctgcgc
     7921 tttgctgcgc ttgggttctt taacgtggac ttcgtggggt gacttgtcgt cgatgggtag
     7981 tgagagaatg gcgtctatgg cgttatttct gattgcttcg ttgcgaagca gaaaggtttg
     8041 cttcatctcc tgctctccgg ttccattttt cagccgccgc agcaactgat ggtgcccatg
     8101 cccccctggc ttcacagagg tcacattctg catagcccca cacatcaata tttattccgg
     8161 cctcaaccca cagacgagca ttaccgccgc aaaacggaca ttcttttagc tttggctggg
     8221 ttaatgatag gtcgctcatg ctcactcctt cactttaaat ccagactccg gataattctg
     8281 ttgcgctgaa actcattgtt gagtttgaac aaccgtcgaa gaacacggtc acgcggatag
     8341 cgtcgtgcgg caggtgaatg ctcatacaac tcatcaagcg gcaaactgga cgatgaacga
     8401 taccgatacc aacgcaccaa ctcctcacga aaattagccc tgacaagctc agctatcgta
     8461 ctcatttctt aaaacctcct caaacgcatt ctgacgcatt tttcattctc gctgcttatt
     8521 ggcatgcctt gcacgcgttt acctcgctac agagcgattg tgatgcctta aaagcgattt
     8581 attgaagtga tatttgctta atcgaaattc ttttctttga ttcctgcggc cctgatggct
     8641 ttcattactg caattaccgt tttgtcacgc ccatcctcat aacccatcgc ataagcacct
     8701 tcttcaccat ctttccaaaa gtcgtcattc gattcgggcc agtcgatatc cagttcaata
     8761 gcagagcgcg atgcctgcca tatcacccag gcaaactctt ttaattcatc gtctcccgtg
     8821 aactggcttt tgtcttttga ccaccagttt tcaaactgtc ggtagctatc gttcacttcc
     8881 ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttattagc tcagtgatgt
     8941 agatggtcat cttttaactc catataccgc caatacccgt ttcatcgctg cactctggcg
     9001 acactcctta aaaatcaggt tcgtgctcat ctttccttcc cgttcttccc tggtagcaaa
     9061 ccggtaatac accgttcgcc agaccttacc atcaatgacc aggattcctg cccgcgccat
     9121 tttagccgca gcctgattta tgctggttac ggttgcgcct gttaccgcgg aaacgtcctg
     9181 tgcacagaag ttcttatgcg tcccgaggta atgaataatt gcctctttgc ccgtcataca
     9241 cttgctcctt tcagtccgaa cttagcttta atttctgcga tcttcgccag cgcctgaaca
     9301 cgatttagag gtctgccgcc catgacagga agttgtttta ctggttcagg tatcgtctca
     9361 ccacggttaa ttcgtgctgt catacaggtc agttcatcgg cagccttgcg ccgtaattcc
     9421 gcgtcagtca gcgcattggc ccgcatgttc tggtacaggt tggtaaccag ccagtagtgc
     9481 gcgtttgatt tccacggata agactctgca tccggataca ggccacgctt ccggcaatac
     9541 tcgtaaacca tatcaaccag ctcgctgacg tttggcagtc cggcgataac ggatgcttct
     9601 tcccggcacc atgcaacaaa ctgcccgggt gatggaagaa atggtcgatt ctgccgacgg
     9661 gctacgcgca ttcctgcgtt aacctgttcc atcgaggtga tcccgttttc ccggaaagcc
     9721 agaacccact ggcggcggat ttcattcagt tcgttctggt cccggttagc cagactcgcc
     9781 gggaaagttg ccagtaactg gctgaacaca ccgttgatga tctgcgctac ctgttgtacc
     9841 tgcggctttt cgtcgtactg ttccggcatg ttgttggcga ttcgacgcat ctgctcacgg
     9901 tcaaagttaa ccatctgtgc ggcgatgttt ttcataaatc caccccgtaa atccagtcag
     9961 tgtttgtcag gtcgagtttt ggtttgctgg ctgtcacgcc tgcctgttgc ttgttacggt
    10021 tgatttcgag ctgggtccac ttgtcgcgga gtttggccgg actcagcacg ttaccggacc
    10081 agaagttgtc ctggcaggcc cagcggaaaa gcacacacat atcgcggtgg ttacgtccgt
    10141 cacgttcacg catcaggcgg atatcgttag cccacccagc aaaattcggt tttctggctg
    10201 atggcgcgat agtcttcacc atgtcaaaca tccactctgc ggcggtcagg tcttctgctg
    10261 ttccccactt gctgccgctc tgaattgcag catccggttt aaccacagaa agatcgtttt
    10321 ctggctggtc agaggattcg ccagaattct cggacgaata atcttttctt ttttcttttg
    10381 taatagtgtc ttttgtgtcc ccctgttttg agggatagca atcccctaat ttgagggatg
    10441 ttttatccct cgttttaggg gattttccct cgttttgagg gatgtccctc attttagggg
    10501 aacctccctc gttttgaggg atgcaccatt ctgagatgtt tttatttggt ccaaacatgc
    10561 cgccttgctg cttgataata ttcattctga cgagttctaa cttggcttca ttgcaccgtt
    10621 tgacaggtaa ctttgtaatc tcgctaagtt gagaatcggt gattctgtcc attggtttat
    10681 tccacccata ggttttacgc agaatggcaa gcagcacttt aaactgtcgc ttggtcagat
    10741 ctgcgcctga ataagcctca atcagcatat ttgatagtct ggcgtaacca tcatcgagat
    10801 ctgccacatt acgctcctgt tcggcaaagt tacctctgcc gaagttgagt atttttgctg
    10861 tatttgtcat aatgactcct gtggattgat ccagtaattc cctcagaatt gcatatcaat
    10921 ttgcttagag tccccggcgg ccaccgggga ttttttcttt gtgattccat caagcgcata
    10981 cttaaaagcc ctactaatcg gactgatgtc tgatgccatt ccgaaagcac acaagaccga
    11041 agcaataaat ctccagtccg ttctgcttat cttcgattca tgacagccaa tcatctttgc
    11101 cagaccgcgc tgggtaagcg ttgacaggtt gatgagtaaa tcagtttcag cgcgatcaat
    11161 ttctcgctgt gatagtttgc tgtaacttgt ttgttccatt tcttaagatt tccaatagtg
    11221 aatagttagt tgaaaggtat gcgtggaaac gcatgtggcc ttagttggtc agatatattg
    11281 ggactcgctt tgtcagcgac gtaggacgaa tgtccattgt gaaaatagcg gtgttactta
    11341 tgcagccgat gctctacgcg atacgaacac taggttttcc tttttcacag gtttataacc
    11401 cgtgaaatta cgagtagctt cttcgattgc attcgcttta tcaggggaag ctcttcgaaa
    11461 tccatatgca atctggtcaa gatagccaac tgaagttttc gctaatgcgg cgagtcgctt
    11521 ccattcctca ctagaagcct cttttcgcca gcgtagtagt tcattactca ttagtgcctc
    11581 cgtttatcac acagaacaaa tttaccattt tgataaatca tccgcaatgt aaatttatca
    11641 tattgcgtat ttatccattt gctaaataga gggaaaattg tgagatggaa aacaaagata
    11701 ttcgcaaatc gaatctggcg tttttgctag atgagcataa aaaaatcgcg ggtaacacta
    11761 atgcaagctt tgccgataag cttggggtta gcccttctca actcacgcaa gtctccggtg
    11821 aaaaaagcac tcgaaacata ggggataaac tagcaagaaa atttgaagcc gcgcttgggt
    11881 tacctaatgg gtggcttgat ttggtacatg atgtaacacc aattgcatca tgctcagatt
    11941 ctttaacttt tgtcggtcag gtaagaaaag ggttagtgcg cgtggttggt gaggcaattc
    12001 ttggtgttga tggtgccatc gagatgaccg aagagcgcga tgggtggctc aaaatttata
    12061 gcgatgatcc agatgccttt ggtcttcgtg tgaaaggaga cagcatgtgg cccagaataa
    12121 aatcaggaga atatgtactc attgagccta acaccaaagt attcccgggt gatgaggtgt
    12181 ttgtcagaac cgttgaagga cacaacatga ttaaggttct tggctatgac agagatggag
    12241 aataccaatt tacaagcatt aaccaggatc acaggcctat aacgttgcct tatcatcaag
    12301 tagcaaaggt ggagtatgta gctggtattc tgaagcaatc tcgccatctg gatgacatcg
    12361 aggcaaggga gtggctgaaa agttcgtgac ttcatcgtca catagctggt aaccagtggc
    12421 ctgaagagac gtttgggtaa ggaggataga tggcgttcaa tgaccttgaa tatcaagcag
    12481 taaaaaaaga agttcaccaa ttcattgaaa gcataaggcc gcctgaacat atccgcaatg
    12541 aactggatat tgtttatagc atcaatgacc aaacgataga tatcggcgaa cagcgccccg
    12601 tgtggcaggg caatccaggt gaaacaaaca tcctgccatc agcaagaatc aagtacatac
    12661 gttctctgga tagatggaaa atctattgga tgcggaagga tatgaaatgg catcagtaca
    12721 gtactgaact ttcgctgact gatgcgcttg agcttgtgcg tgctaacccg gattgctgct
    12781 tcttcggatg agtgaagaga cgtttggatg atggatggtc gcagagatgc ggcctgattc
    12841 taaaatagga tataaaaaat gagaatacta ggtgttagag cggcgcccaa agttacatct
    12901 tttgttgtat attgcactaa tgagtctgca ctcaaatgtg ttgatgttat taaaatacct
    12961 tcgaccttag acacaccaga aaaattaaag tatgtgagaa ataacatcct cgacattctt
    13021 aatttatata atgttgaatt agctgccata cgcgttactg aatcaaactc tgataatctt
    13081 agcattgacc gcctttatat agaagctgtt attcaagaag cattttcaag cagtgatgta
    13141 agaaaatatt acactattag aaaatctggc atgaaatcat cattgaacct aacagagatc
    13201 gagtataaag aaatattgaa gtcacaccgc aatataaatg gaatcgataa ttctggtttt
    13261 acaactgaaa caaatgaagc tgttttggct gcactatctg cggaggtaag gggatgctaa
    13321 ctccatacaa aagagctgat gtagaattcg aatggattag tgatctagaa gaacagggtt
    13381 gtttttcaaa agtatatctg gctcatgaca gacacctagc tcatgacttg gtgattaaag
    13441 aaatagaaaa aaaagaaaac actaaccacg acgactactt taatgaagca aggcttctct
    13501 ataaacatgc acatccaaat attgtgcaag ttcagtatgc tgctcaatgt gagagcaata
    13561 tctatatagc catgccattt tatcataatg gttcgctaaa ccaattaatg aaaaaaaata
    13621 atcttacaag cagggagata atacggtatt ccattcaatt tttaagtgga ctttatcata
    13681 tacactcaaa aggtcttatg cattttgata taaaacctaa taacattatg atatcaaaca
    13741 gaaatgaggc catgctatct gactttggat tatctcagtt agtcaatgag gaatcgagag
    13801 ctgcgcctga gtttggatat cattttcatg tgccaccgga atatttttct ttatcaacaa
    13861 atgattataa tttcacatat gacatatatc aggcaggatt aaccatatat agaatgtgtg
    13921 ttggacatga taattttgaa agagaaagat ctgcatttag cacgattgaa caactcagag
    13981 agtcgataat taatggctgc tatccattaa aagagtatcc tccccatata cataaaaaat
    14041 taataacaat agtgaacaaa tgcattcatg tagatccaaa tgaaagatat caatccgtac
    14101 tagatgtact aaacgatctc tcagctataa gtgatggcgt tcttgactgg cgtctacaga
    14161 tgacgaaacc aactaacggc acatgcgaat ggcaaaaaaa gtctggggac gctatactgt
    14221 ctatagtttt tgacgcagaa aattcgtcta ctactggttt tcgtttatac gatgatgggc
    14281 ggaaaaggcg tgctacgaac ttaacaatat cctcaggatg tacccctaca aaactgtata
    14341 ggttattaaa ggataactga tcatgaaaaa gcgcgaggaa gtaagcaagc tgcctcgcag
    14401 acgtgatgca gcattagcgg ttccctacaa aaaagatgag ttcataagcc cttctgatga
    14461 caaaaaattt tcaaaggcga aaagttttac atctacatct ctaaaagata aatactttaa
    14521 aatctagccc ggcctcagcg ccgggttttc tttgcctcac gatccccaca cctaaaaaca
    14581 cataaccaat tgtatttgtt gaaaaataaa tagatacaac ttgctaaaca acgcaatcca
    14641 gatctccctc aaatctcttt atttatcctg tcgaattcct acaacaaaat aaaacaccat
    14701 aagaatcaat acgatatttg aaaaccaaga gaatttatca ttttgctatt gccattaatt
    14761 tatcattccg ataaagttca cccatcagca ggacgcacta ctcaccaggg cggtgaatat
    14821 acaacgattc gaatatgaat ctacggcgct gacaaagcgc aataaccaaa gtgaactttg
    14881 gggtgtggtg aagggttcat ggacgggaat atgtcgcacg taaagcggcg aggcctgcgg
    14941 gactattgcc gaattgaagt aggccgaaac aggtcgaaat gggtctccca cctaccacac
    15001 caccaaagtt catcaggagg tctatatgac acgcagaact cagttcaaag gcaattcacg
    15061 ttctcgtcgt cgtgagcgtt taaaggcaaa ggcattagct aacggcgtgc tggcccgcga
    15121 agaagcaata agttcagaag tattacaccg ccctactcta agcagagcgc agattcaggc
    15181 taaaggtact cacgaaacgc ctgagcgcat agaagacgct aagccaatta agttcatggc
    15241 acaggacgtg atctggcaac agaaagaata cagacgcaat ctggagcgag cggccattgt
    15301 gtacgcgaat gagtttggac ataagcaacc agaaactggt gtatgtcttc caaacgtagc
    15361 tctttacgcg gcaggctacc ggaaatcaaa acaactgacg gcaagatgac ttgtgttggt
    15421 cgccagaaaa tgaaattagg cagcaaacca cttatttgag aggaattaat atgtcatcaa
    15481 tccgcttaac tacgagaatg aaagaggaaa tcgctcgtaa cgctttaatt aagtccgggg
    15541 tgttcactga acttgaagaa gtaacaaagt taaagaacca gcttgcactt gacgccagag
    15601 ttattgcgtt tggcggtaaa aagaaaactg aggaagtgga tcggttatca tccaagttgg
    15661 tagctataag tgaagaactt ggaaagatgg gatgttcatt ttactcatac gatgttagtt
    15721 ctacttcaat ttatctgact gtatctggca gaagggttgg atggcattca tatgggaaag
    15781 acggcaacgg caaagatata ttgctcccta ctccgatcaa agataaatgc atgtttgacg
    15841 cagaacacga aataacaaaa aggtttgatg aaatctgcgc attgcaacaa aaacttgaag
    15901 ccaagaaaaa ggatatcgaa tcaaatgtat gggctgcttt gaactcagtc acaacagtta
    15961 agcgacttat tgaagtttgg cctgaaagca aagaattgct accaaaagaa gcagataaag
    16021 caagtacagc acttcctgct ttacgggtag aagatttgaa taagatgatt ggacttcctt
    16081 ccgaggccgc atagtcggcc tttatttttg gcataaacaa cagaataaat actgcactgt
    16141 gtattcattc caacgagtga atacacggag caatgtcgct cgtaactaaa caggagccga
    16201 cttgttctga ttattggaaa tcttctttgc cctccagtgt gagggcgatt ttttatctat
    16261 gaggatatga a
//
LOCUS       DAZGCS010000107        16244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGCS010000107 DAZGCS010000000
VERSION     DAZGCS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.107"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..123
                     /locus_tag="ACHK01_003854"
     CDS             <1..123
                     /locus_tag="ACHK01_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098728.1"
                     /translation="KIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPLNYLYL"
     gene            459..1436
                     /gene="glaH"
                     /locus_tag="ACHK01_003855"
     CDS             459..1436
                     /gene="glaH"
                     /locus_tag="ACHK01_003855"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO8098729.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1456..2724
                     /gene="lhgO"
                     /locus_tag="ACHK01_003856"
     CDS             1456..2724
                     /gene="lhgO"
                     /locus_tag="ACHK01_003856"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO8098730.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTSQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPRPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2747..4195
                     /gene="gabD"
                     /locus_tag="ACHK01_003857"
     CDS             2747..4195
                     /gene="gabD"
                     /locus_tag="ACHK01_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8098731.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4209..5489
                     /gene="gabT"
                     /locus_tag="ACHK01_003858"
     CDS             4209..5489
                     /gene="gabT"
                     /locus_tag="ACHK01_003858"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO8098732.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5727..7127
                     /gene="gabP"
                     /locus_tag="ACHK01_003859"
     CDS             5727..7127
                     /gene="gabP"
                     /locus_tag="ACHK01_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO8098733.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7148..7810
                     /gene="csiR"
                     /locus_tag="ACHK01_003860"
     CDS             7148..7810
                     /gene="csiR"
                     /locus_tag="ACHK01_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO8098734.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7811..8260)
                     /gene="kbp"
                     /locus_tag="ACHK01_003861"
     CDS             complement(7811..8260)
                     /gene="kbp"
                     /locus_tag="ACHK01_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO8098735.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8344..8502)
                     /gene="yqaE"
                     /locus_tag="ACHK01_003862"
     CDS             complement(8344..8502)
                     /gene="yqaE"
                     /locus_tag="ACHK01_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8098736.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8685..8984
                     /gene="ygaV"
                     /locus_tag="ACHK01_003863"
     CDS             8685..8984
                     /gene="ygaV"
                     /locus_tag="ACHK01_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8098737.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8994..9518
                     /gene="ygaP"
                     /locus_tag="ACHK01_003864"
     CDS             8994..9518
                     /gene="ygaP"
                     /locus_tag="ACHK01_003864"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO8098738.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9565..9969)
                     /gene="stpA"
                     /locus_tag="ACHK01_003865"
     CDS             complement(9565..9969)
                     /gene="stpA"
                     /locus_tag="ACHK01_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO8098739.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10637..11086
                     /gene="alaE"
                     /locus_tag="ACHK01_003866"
     CDS             10637..11086
                     /gene="alaE"
                     /locus_tag="ACHK01_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO8098740.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(11123..11467)
                     /gene="ygaC"
                     /locus_tag="ACHK01_003867"
     CDS             complement(11123..11467)
                     /gene="ygaC"
                     /locus_tag="ACHK01_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO8098741.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11619..11948
                     /gene="ygaM"
                     /locus_tag="ACHK01_003868"
     CDS             11619..11948
                     /gene="ygaM"
                     /locus_tag="ACHK01_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO8098742.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            12196..12441
                     /gene="nrdH"
                     /locus_tag="ACHK01_003869"
     CDS             12196..12441
                     /gene="nrdH"
                     /locus_tag="ACHK01_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO8098743.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            12438..12848
                     /gene="nrdI"
                     /locus_tag="ACHK01_003870"
     CDS             12438..12848
                     /gene="nrdI"
                     /locus_tag="ACHK01_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO8098744.1"
                     /translation="MSQLVYFSSNSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12821..14965
                     /gene="nrdE"
                     /locus_tag="ACHK01_003871"
     CDS             12821..14965
                     /gene="nrdE"
                     /locus_tag="ACHK01_003871"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8098745.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14975..15934
                     /gene="nrdF"
                     /locus_tag="ACHK01_003872"
     CDS             14975..15934
                     /gene="nrdF"
                     /locus_tag="ACHK01_003872"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8098746.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         3910 a         4096 c         4206 g         4032 t
ORIGIN      
        1 aaaattatcc cccctggtaa agcatttatg cttagaggcg gaactctaaa tatcaatatt
       61 cctgggcgta gcgctcttct tttagggaaa acaggagaac cgctgaacta tctctatttg
      121 taatttaatg ttatatctgc cccgctaaaa cggggcagat aatatgttta gtttactaac
      181 ggtcattttg cattgaagcc atttactgtt ttttatcgac cagataatct gttctctgat
      241 ggtaactccc cctaacctat tgctttagtt attcatttcc tgtctcactt tgccttaata
      301 ccctacgtta aatgttacta atttgttgct tttgatcaca ataagaaaac aatatgtcgc
      361 ttttgtgcgc atttttcaga aatgtagata tttttagatt atggctacga aatgagcatc
      421 gccatgtcac cctacatctc ataagaggat cgcttctgat gaatgcactg accgccgtac
      481 aaaataacgc tgtcgattca ggccaggact atagcggatt cactttcatc ccgtcggcgc
      541 aatccccgcg tctgctggaa cttaccttca ccgaacagac gaccaaacag tttctcgaac
      601 aggttgccga atggcccgta caggcgctgg agtacaaatc ctttctgcgt tttcgggtag
      661 gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc attgctgttg aagaccctgc
      721 taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg tatcgatgat gtggcgcagg
      781 cagatgagat ggtgaagctg gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg
      841 atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa aaatatcgat aactcagaca
      901 gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag
      961 agatcaccga ttacgtgctg atgatgaaaa tcgatgagca aaacatgcag ggtggaaatt
     1021 cgttgctgct gcatctcgat gactgggaac atctggacca ctatttccgc catccactgg
     1081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     1141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     1201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     1261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     1321 tattctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     1381 tgcgtcagcg tggctatttc gcttacgcct ctaaccacta ccagacgcat cagtaagcgc
     1441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     1501 accgccatgc aactgattga tgtctatccg gatgcccgca ttgcgttgct ggaaaaagag
     1561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     1621 tacacgcccg gtagcctgaa ggcacagttt tgcctggcgg gaaaccgcgc cactaaagcc
     1681 ttttgcgatc aaaacggcat tcgctttgac aactgcggca agatgctggt cgccacgtct
     1741 ccgctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     1801 cgcgaatggc taaacgccga cgaactgcgc gagcgcgaac cgaatatcac cgggctcggc
     1861 ggtatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacagc ggcgatggca
     1921 aaaatctttc aggacagagg cggcgagatt atttataacg ccgaagtcag cgcccttaat
     1981 gagcataaaa acggcgtggt gatacgtacc agtcagggcg gtgaatatga agcatcgacg
     2041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg actcgaaccg
     2101 ggctttatca tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     2161 attgttaacc acctgattta ccccatcccc gacccggcaa tgccgttttt gggcgtgcat
     2221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     2281 cgtgaaggct atcgcaagcg cgacttctca tttagcgaca cgctggaaat tttgggctcg
     2341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     2401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     2461 agcgatctcc agcccaggcc cgccggtgtg cgggcgcagg cggtatcgcc ggacggcaag
     2521 ctgattgacg attttctgtt tgtcaccacc ccgcgcacga tccacacctg caatgcgccc
     2581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     2641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     2701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     2761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     2821 cgaggtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     2881 gggcgcggat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc cagcctggcg
     2941 cgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgga
     3001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     3061 gaaaggcgaa atcagctacg ccgcttcctt tattgagtgg tttgccgaag aaggcaaacg
     3121 catttatggc gacaccattc ccggtcatca ggccgataag cgcctgattg ttatcaagca
     3181 gccgattggc gttaccgccg ccatcacgcc gtggaacttc ccggcggcga tgattacccg
     3241 taaagccggt ccggcgctgg cggcaggctg cacgatggtg ctgaaacccg ccagtcagac
     3301 gccgttctct gcgctggcgc tggcggagct ggcgattcgc gcgggcattc cggctggcgt
     3361 atttaacgtg gtcaccggtt cggcgggcgc ggtgggtaac gaactgacca gcaacccgct
     3421 ggtgcgcaaa ctgtcgttta ccggttcgac cgaaattggc cgccagttaa tggaacagtg
     3481 cgcgaaagac atcaagaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     3541 tgatgatgcc gatctcgaca aagccgtgga aggcgcactg gcctcgaaat tccgcaacgc
     3601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     3661 tgccgaaaaa ttacagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg
     3721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     3781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcggatg aacgcggcgg
     3841 caacttcttc cagccgacca ttctggtgga cgttccagcc aacgccaaag tgtcgaaaga
     3901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     3961 gcaagccaat gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg
     4021 cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcatcat
     4081 ttccaatgaa gtggccccgt tcggcggcat taaagcctcg ggtctgggtc gtgaaggttc
     4141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     4201 gaacgcgaat gagcagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     4261 gtgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     4321 ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcact
     4381 tgcatccgaa agtggtggct gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     4441 tccaggtgct ggcctacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     4501 caggcaattt tgccaagaaa acgctgctgg tcaccacagg ttctgaagcg gtggaaaacg
     4561 cggtgaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt
     4621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     4681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     4741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     4801 aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgcctcgt
     4861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     4921 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     4981 gcgttgcgcc ggatcttacc acctttgcga aatcgatcgc gggcggcttc ccgctggcgg
     5041 gcgtcaccgg gcgcgcggaa gtaatggatg ccgtcgctcc aggcggtctg ggcggcacct
     5101 atgcgggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg tttgagcagg
     5161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagtt gaaagacgga ttgctggcaa
     5221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggggcgatg atcgccattg
     5281 agctgtttga agacggcgat cacaacaagc cggacgccaa actcaccgcc gagatcgtgg
     5341 ctcgcgcccg cgataaaggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     5401 gcatccttgt accgctcacc attgaagacg ctcagatccg tcagggtctg gagatcatca
     5461 gccagtgttt tgatgaggcg aagcagtagc gccgctccta tgccggaggc gacgctgcgc
     5521 gtcttgtccg gcctacgggg atccaggtcg gataaggcgt ttacgccgca tccgacaatc
     5581 tgtacgtgaa caggaagaaa tctatgttgg ccgggtaagg cggagccgct ctccggcaaa
     5641 aagaatcaat aacaattata cgcgtgaccc ggcgcgggaa atgtcggggc gctctcccaa
     5701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg
     5761 gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     5821 tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc
     5881 tgttcgccgg gctactggtg gttatgatta tgcggatgtt ggcggaaatg gcggttgcca
     5941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggtcgc tgggcgggct
     6001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccgctg gaagccaaca
     6061 tcgccgccat gatcctgcac tcatgggttc caggcattcc catctggtta ttttccctcg
     6121 tcattaccct cgccttaact ggcagtaact tattaagcgt taaaaactac ggcgaatttg
     6181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     6241 tcgcaattag cggtttttac ccgtatgccg aagtgagcgg gatctcaaga ttgtgggata
     6301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     6361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac acgccggaaa
     6421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttatgtt
     6481 ctatttttgt cgtcgtggca ttaattccgt ggaatatgcc cggactaaaa gccgtcggtt
     6541 cttatcgctc ggtactggaa ttgctcaata ttccccatgc gaaattaatc atggactgcg
     6601 tgatattact ttccgtaacc agttgcctga actcggcgct gtataccgcg tcaaggatgc
     6661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     6721 aaaccccgta cgtggcggtg ttactctcca ccggcgcggc attcttaacg gtggtggtga
     6781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     6841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     6901 aaggaagcga aattcgcttg cgaatgtggc tttatccgtg gctcacctgg ctagtcatcg
     6961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
     7021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
     7081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgatca tgatcaggag
     7141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
     7201 cggtaatttt caaccggatg aaaaattacg aatgagtttg ctgacatcgc gttatgcact
     7261 tggcgttggg ccgttacggg aagctctttc gcaactggtg gcggaacggc tggtcacggt
     7321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagttgc tcgatatttt
     7381 cgacgcccgc gccaatatgg aagcgatgtt agtgagtctg gcgattgccc gcggtggcga
     7441 tgagtgggag gcagacgttc tcgcaaaagc gcatctgctg agtaagcttg aggcctgtga
     7501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacggcaat
     7561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
     7621 ggcgcgctat cgatttatct ggctgcggcg aacggtgctt tcggtagaaa tgctggagga
     7681 taaacacgat cagcaccaga ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc
     7741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
     7801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
     7861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
     7921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
     7981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccaa
     8041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
     8101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
     8161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga
     8221 gtttttctcc ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg
     8281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
     8341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
     8401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag
     8461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
     8521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
     8581 gccagtttca gcttacgctt ttactctcat ttacgctgct gaggctggta tatgttgcat
     8641 ttttagttta gagtttactt aatttagaaa atacttaaat aaatatgact gaactcgcgc
     8701 aattacaggc cagtgccgaa caggcagcgg ccttattgaa agcaatgagc catcctaaac
     8761 gattgctgat tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc
     8821 gcattaccgg actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag
     8881 ggcttatcga cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg
     8941 taaatgccat tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt
     9001 tgacaaccat ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg
     9061 atattcgtga cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat
     9121 tatccgtgct ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc
     9181 actgccaggc aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc
     9241 ccgcagaaat atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag
     9301 tagcggtaaa taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg
     9361 gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa
     9421 gtggctttgt tggtgccggg ttactgtttg caggaatcag cggtttttgt ggaatggcaa
     9481 ggttgttaga taagatgccg tggaaccaac gaacttgaga agcgacgccg gacgcgccct
     9541 agcagcgaca tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc
     9601 cagcgcctga gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc
     9661 gttaacatcg gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcgcgtgg
     9721 tgcagcagca gagctattac ctaataactc ttccgggtta attccgtcag ccttcatcag
     9781 ctccagccag gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc
     9841 ttcttcacgt ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc
     9901 aatggagaat tcgcgagcca tcgcacggag agtgcgaatg ttatttaaac tttgtaacat
     9961 tacggacata ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt
    10021 gtaagcgatc acttattata tttccacaaa atttaatcta tttacagtcc tgtgcgagat
    10081 tatttcagct gttattttct gaaactctca gaataatttc aagattatat attagccatt
    10141 ttgatgagta atttagatta ttggtgaaca gaacggcgct ctctgctcgc taattccgca
    10201 agcctgacaa aaccgaagtg aataaaaatg caatataaca gaataaaaga aaacacccct
    10261 cactggtgct ttctgctgtt aatgtacatt ttctggcata aaaaatactg taaatgacac
    10321 ggtcacgaca tacaccgaat tctctacgct tatagcgtta cctcaccccc aaactttcat
    10381 tagtgaaatt aaaggaaagg tcatatctaa ttgatttaat tgaaaataaa tcatatcggc
    10441 atgtaaaaat ccacaatgta caaaaaacga gccgttacgg aatattttat ctacaaaaac
    10501 tgactaaata aaaatttttc actaattgaa tagtcatagc cagcgatata cgctatgcga
    10561 aaatgcagat ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt
    10621 attaaggagc attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac
    10681 gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg
    10741 aatgagcttc gaacagtctt tttattccag attggtagcg attccggtga acatcttaat
    10801 tgcatggcca tacggtatgt accgcgatct gtttatgcgc gcggcacgca aagttagccc
    10861 gtcgggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt
    10921 gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag
    10981 ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg
    11041 ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct tcttttcgcg
    11101 actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt
    11161 caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt cgctcgcctg
    11221 ctaaatagag cggaaattgc tggtaatgat cgcaagtttt aatatcggaa gcgggttcag
    11281 ccagtgttga actgcgttct tttaatgttg gatgaattac cagagcggta cgtcccattc
    11341 gcgcttcgcg attaacatag acataatttt ccccacggcg gtaaccatac gctttctggg
    11401 ttaccacatc gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta
    11461 aatacatatt tgatcctcgt tattatcaac cgcgggccta ccttacctga ttgcgcattc
    11521 acaacgcttt cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc
    11581 tacactgaaa attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca
    11641 acgacgttga tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagcc
    11701 tcgaatctgt attgaagtcc tggggcagcg acgccaaagg ggaagctgaa gccgcacgca
    11761 gtaaagctca ggcattgctg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc
    11821 agcaagccgc acgcgatgcc gttggctgcg cggattcttt tgttcgtgaa agaccctggt
    11881 gtagcgtggg tacagcagct gcggtaggta tttttatcgg cgcactgtta agcatgcgca
    11941 aatcgtagtg caaaaatgat aataaatacg cgtctttgac cccgaagcct ggcttcgggg
    12001 tttctttttg cctggtgaat cacaaaaatc cccctacccc gtcacgctca tatccagggt
    12061 aatttcgacc actatttgct atatattgtg tggttgaatc ttttttcaac tacatctagt
    12121 atctctgtat caacagagat acaacccgac gcgtatcatc gcgccgtatc ttcattttaa
    12181 acggaaatac gaatcatgcg cattactatt tacactcgta acgattgcgt tcagtgccac
    12241 gccaccaaac gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc
    12301 gttcctgaag cagcggaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt
    12361 gctggcgatc ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg
    12421 ccacacgcgg ccagtgcatg agccagctcg tctacttctc cagcaactcc gaaaacacgc
    12481 agcgttttat cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac
    12541 ggattcaggt agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg
    12601 ctggcgcggt gccacgacag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc
    12661 ttcgcggcgt tattgcttct ggtaatcgca actttggtga ggcgtatggc cgcgccggag
    12721 atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc
    12781 aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga
    12841 atgcctgacg caggaaacga tggattacca cgcgctgaat gcgatgctta acctctacga
    12901 tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttattgcgac
    12961 gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt
    13021 caacgaaggt tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac
    13081 gctgtttgcc cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa
    13141 gttttacacc agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc
    13201 cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc gatgcgacgc tggcgttgca
    13261 actgaccgat gaaatgctgt caggacgctt tcagccagcc acgccaacat tcctcaactg
    13321 cggtaagcag cagcgcggcg aactggtttc ctgttttctg ctgcgtattg aagacaatat
    13381 ggagtcgatt ggccgggcgg taaattccgc actgcaactg tcgaaacgcg gcggcggcgt
    13441 ggcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca
    13501 atcttctggc gtgattccgg tgatgaaaat gctggaagac gcattttcct atgccaacca
    13561 gctcggcgct cgtcaggggg ctggtgcagt ctatttacat gcccatcatc ccgatattct
    13621 gcgttttctc gatacgaaac gggaaaatgc cgacgagaaa atccgcatta aaacactgtc
    13681 gctgggcgtg gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc
    13741 gctgttttcg ccttatgacg tagagcgagt ttatggcaag ccgtttgccg atgtggccat
    13801 cagccaacac tatgacgaac tggttgccga tgaacgcatt cgcaaaaaat acctcaacgc
    13861 ccgtgatttc ttccagcgac tggcagaaat ccagtttgag tccggctatc cctacatcat
    13921 gtatgaagac acggtaaacc gtgctaaccc tatcgccggg cgcataaata tgagtaatct
    13981 ctgctcagaa attttgcagg ttaacagcgc ctcagagtat gacgagaatc tcgactatgc
    14041 ccgtacaggc catgatattt cctgcaattt aggttcgttg aatattgccc acaccatgga
    14101 ttcccccaac tttgcccgca cggtagagac tgccgtacgc ggtttaacgg cggtgtcaga
    14161 tatgagtcat atccgcagcg tgccgtccat cgaagccgga aatgccgcct cacacgccat
    14221 cgggctgggg cagatgaatt tacacggtta tctggcgcga gaaggcatcg cttatggttc
    14281 gccggaagca ctggatttca ccaatctcta tttctatacc atcacctggc acgcactgcg
    14341 tacctcgatg ttgctggcac gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg
    14401 ttatgccagt ggtgaatatt tcagccaata tctgcaaggt aactggcagc cgaaaacggc
    14461 gaaagttggc gaactgtttg cccgcagcgg tattacgtta cctacccgtg agatgtgggc
    14521 gcaactgcgc gacgacgtga tgcgctacgg catttataac cagaatcttc aggcggtgcc
    14581 gccaaccggt tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc
    14641 gaaagtagag atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat
    14701 gactaacgag aatctggcgc tgtatcagga tgcttacgaa atcggcgcag aaaagatcat
    14761 cgacacctac gcggaagcga ctcgccatgt cgatcagggt ttgtcgctga cgctgttttt
    14821 ccccgatacc gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg
    14881 tatcaaaacg ctctattaca ttcgcctgcg tcagatggcg ctggaaggca ctgaaattga
    14941 aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat
    15001 caactggaac aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa
    15061 tttctggcta ccagaaaagg tgccgctgtc gaacgatatt cctgcctggc agacattaac
    15121 tgtcgtagaa caacaactga cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct
    15181 gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgca ctcacgcctc atgaagaagc
    15241 ggtattatcg aatatcagct ttatggaagc ggttcatgcc cgctcttaca gttcgatttt
    15301 ctcgacgctg tgtcagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc
    15361 accgttgcag cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa
    15421 aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc
    15481 gatgtatttt tccagccgcg gaaagctgac caataccgca gatctgatcc gtctgattat
    15541 ccgcgatgaa gcagtccacg gttactacat aggctataaa tatcagaaaa acatggaaaa
    15601 gatatctctg ggacaacgtg aagagttgaa gagtttcgcc ttcgatttat tgctggaact
    15661 ctacgacaac gagttgcaat acaccgatga gctgtacgcc gaaaccccgt gggctgacga
    15721 tgtgaaagcg tttctctgtt acaacgccaa taaggctttg atgaatctgg gctacgaacc
    15781 gttatttccc gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa
    15841 tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc
    15901 ggttgaaaca gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta
    15961 cccgcagcag ggaaataatt cccgccaaat agctttttat cacgcaaata ttctgtagtg
    16021 atctacactg atactctgtt gcattattcg cctgaaacta caatattcag gcgttttttc
    16081 gctatctatg acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacggg
    16141 aaattccggc gatttgctcg catcaatatt catgccacat ttgccctcag gggttgcctc
    16201 agattctcag tatgttaggg tagaaaaaag tgactatttc catt
//
LOCUS       DAZGCS010000108        16004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGCS010000108 DAZGCS010000000
VERSION     DAZGCS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.108"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(101..2134)
                     /gene="betT"
                     /locus_tag="ACHK01_003873"
     CDS             complement(101..2134)
                     /gene="betT"
                     /locus_tag="ACHK01_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO8098747.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2263..2850
                     /gene="betI"
                     /locus_tag="ACHK01_003874"
     CDS             2263..2850
                     /gene="betI"
                     /locus_tag="ACHK01_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO8098748.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2864..4336
                     /gene="betB"
                     /locus_tag="ACHK01_003875"
     CDS             2864..4336
                     /gene="betB"
                     /locus_tag="ACHK01_003875"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO8098749.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4350..6038
                     /gene="betA"
                     /locus_tag="ACHK01_003876"
     CDS             4350..6038
                     /gene="betA"
                     /locus_tag="ACHK01_003876"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO8098750.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKKMSRDVN"
     gene            6235..6903
                     /locus_tag="ACHK01_003877"
                     /pseudo
     CDS             6235..6903
                     /locus_tag="ACHK01_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7146..7841)
                     /locus_tag="ACHK01_003878"
     CDS             complement(7146..7841)
                     /locus_tag="ACHK01_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8098751.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7834..9261)
                     /locus_tag="ACHK01_003879"
     CDS             complement(7834..9261)
                     /locus_tag="ACHK01_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8098752.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEANGESFRSWFKKHLAQEKKNG"
     gene            complement(9272..9991)
                     /locus_tag="ACHK01_003880"
     CDS             complement(9272..9991)
                     /locus_tag="ACHK01_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8098753.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10518..11372)
                     /gene="rclR"
                     /locus_tag="ACHK01_003881"
     CDS             complement(10518..11372)
                     /gene="rclR"
                     /locus_tag="ACHK01_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO8098754.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11598..12923
                     /gene="rclA"
                     /locus_tag="ACHK01_003882"
     CDS             11598..12923
                     /gene="rclA"
                     /locus_tag="ACHK01_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO8098755.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILHDQIFTHPSMSESLNDLFSLVK"
     gene            13032..13268
                     /gene="rclB"
                     /locus_tag="ACHK01_003883"
     CDS             13032..13268
                     /gene="rclB"
                     /locus_tag="ACHK01_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO8098756.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13280..13873
                     /gene="rclC"
                     /locus_tag="ACHK01_003884"
     CDS             13280..13873
                     /gene="rclC"
                     /locus_tag="ACHK01_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO8098757.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14033..14902)
                     /locus_tag="ACHK01_003885"
     CDS             complement(14033..14902)
                     /locus_tag="ACHK01_003885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8098758.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLAKFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            15151..>16004
                     /locus_tag="ACHK01_003886"
     CDS             15151..>16004
                     /locus_tag="ACHK01_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8098759.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         4225 a         4048 c         3786 g         3945 t
ORIGIN      
        1 tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc aatgattccc
       61 gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca
      121 cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc tcgtactggt
      181 caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg ccctggctgc
      241 cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt gcgcggtagg
      301 taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt tgttcttcgc
      361 ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc ggcaggcttt
      421 ttagctccac gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc attgccgggt
      481 aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg
      541 agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggt gcggtatcgc
      601 ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac cccgccatca
      661 cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg
      721 atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc gaccagaaga
      781 cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg aaattcccca
      841 gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta atggtggcga
      901 cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtaaaag ccgcgctccg
      961 gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc
     1021 tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc aacgtgccca
     1081 gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag ccgacaaacg
     1141 gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc cactcaactg
     1201 gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa
     1261 cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat acgaacagga
     1321 tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc ttatcgacac
     1381 cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc gcttttgccg
     1441 ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc accacaccga
     1501 taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata tccactgagt
     1561 gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag cggatggtga
     1621 gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc gcatacatcg
     1681 accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa
     1741 tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg gctacggaga
     1801 agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa ctcagcaggc
     1861 tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc gaacaagcga
     1921 tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa ccgaaggttt
     1981 tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga aacaggatcg
     2041 ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc ggattgattt
     2101 tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt tattgttacc
     2161 atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc aatcaataac
     2221 cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg
     2281 cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc
     2341 atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc
     2401 agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga catcaccagt
     2461 cagttacgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag
     2521 cgattacagg cgattgttgc cggaaacttc gatgaaactc aggtgagcag tgcggcgatg
     2581 aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag
     2641 caggtcagca gccgccgctt gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg
     2701 cgcgaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg
     2761 cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac
     2821 tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga
     2881 acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac
     2941 cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc gcgaggatgt
     3001 cgatcgcgcc gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat
     3061 ggaacgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact
     3121 cgcaaaactt gaaaccctcg acaccggaaa agcatattcg gaaacctcaa ccgtcgatat
     3181 cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggctc tggaaggcag
     3241 ccagatcccg ttgcgtgaga cgttatttgt ttatacccgc cgcgaacctc tgggtgtggt
     3301 ggcggggatt ggcgcatgga actacccgat tcagattgcc ctgtggaaat ccgccccggc
     3361 gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt
     3421 aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc
     3481 gggggtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc
     3541 atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct
     3601 gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg atgatgcgga
     3661 tctcgatctc gccgccgata tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg
     3721 taccaatggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat
     3781 tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg
     3841 cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga
     3901 ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct tcgataacgg
     3961 cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga
     4021 agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg aagtgattcg
     4081 ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg acctgaaccg
     4141 cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga
     4201 atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg
     4261 cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt
     4321 ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg
     4381 gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc
     4441 tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg cccgcagcgc
     4501 tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag cctgaaccgt
     4561 ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga
     4621 tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg gcgcaagaac
     4681 ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag gccgagactc
     4741 gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc accacctcca
     4801 aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcaggctacc
     4861 cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg gatcgcaccg
     4921 tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc
     4981 gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt gacggcaaac
     5041 gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca
     5101 acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg
     5161 gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg
     5221 gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac
     5281 cggtttccct ctaccctgcc ctgcagtggt ggaaccaacc gaaaatcggt gcggagtggc
     5341 tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga tttattcgca
     5401 gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact
     5461 ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct
     5521 cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcgattc
     5581 tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca attcgcatca
     5641 cccgcgagat catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa atcagccccg
     5701 gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg
     5761 ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg
     5821 aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga
     5881 ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga
     5941 ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc
     6001 cggtgagagc gaaaaaaatg agtcgtgatg tgaactgact caagaaccga tcagattgag
     6061 agttaccgtt ccaggcatgg ggacagaatc gttatataaa acataggggt aactctcatg
     6121 cttcatatcc agtattcatg atgctggctt tttgctgata tttaacccga acattcctga
     6181 tacagactat taatacaacc ttatctgcga cagattttat tttctggata tattatgcgt
     6241 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaaggtt
     6301 cgtgttatcc tttttattat agctatctgt atggcaatga tgcttttatt tctgtatatc
     6361 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accagacata
     6421 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccgcaatgac taaagatgtt
     6481 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6541 ctggaatggt catgagataa gagagattta ccaactgctg aatcggaaat atctcgcgtg
     6601 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6661 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6721 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6781 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6841 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6901 taataaacaa cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6961 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     7021 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     7081 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7141 ctctctcagc aatcctcaat aatcagatac accgctttca ccgggccatg aaccccgacg
     7201 actttgataa gctcaatatc cgccgttgaa ctagggccgc taatgatgtt aatgcaggac
     7261 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7321 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7381 cgcccgcgct cggcggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7441 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7501 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cgctaatccc caattcctct
     7561 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7621 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7681 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gtgcggtaag ccgctcgtta
     7741 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcaacgg gcgacccagt
     7801 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgccag
     7861 atgtttctta aaccaactac ggaaactctc tccgttagct tcaggaagat cgcgtgcttc
     7921 catccagtca ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7981 tgccgcatga gcaccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     8041 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     8101 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8161 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8221 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8281 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8341 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8401 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8461 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8521 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8581 cattcgcgca ttaccttcat tggtcaccag gcataccgaa cctgtctctg ccaccgcgaa
     8641 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8701 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8761 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8821 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8881 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8941 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     9001 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     9061 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9121 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9181 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9241 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9301 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9361 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9421 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9481 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9541 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tatactgctc
     9601 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9661 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcaggt tcatccgcca
     9721 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9781 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9841 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9901 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9961 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
    10021 tatgcccgat gtttcccccc caccggggga tcaatcatca ataaatatgg cgttcttttt
    10081 taattggcta gcaacatacc acccctcatt tgtatgacaa atgataatct ttcatccaaa
    10141 cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta tggggaaatc
    10201 gacgttcatt tttggcataa gaaatccatt taaaaaggct acatccctta tacctattca
    10261 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
    10321 aataatcacg tatcaagtaa tagtcggggt tttatttaat tgaagcaatt tagtttgcat
    10381 ttttatttaa aaactgagct agagtccatc agaaaaaaat atataaagcc caaaccaccg
    10441 ttggaaatag aaatagttga aaggctcaca actcccggaa tgcggattat ctttgccctg
    10501 atttctggcg gttttattca gggtgcaagc tgtctgaccc tttcccgata ttctcccgga
    10561 gtacaaccaa actcgcggac aaacgccttg tgaaaagatg attcactggc atagcctact
    10621 gactcagcga tcaccacaac agggagcatt tcccgggaaa acatctgggc cgctatttgt
    10681 agacgcaact ttgttaatac agccagcggc gtggttccgg aaacatcacg gaaaagctgg
    10741 gcaaaacttg cccgggacat gtgagcgatg ctggccagcg attcgacggt ccaggcgtgt
    10801 cctggcattt ccagcatttg ctgtattacc gcaccaagac gtggatgcag aagcaaactg
    10861 agaatgtttt tctcagtatt aacctgtgca atccactcac gcaccgcgag ggtaaagaat
    10921 gtagcgcaga tctggctaca cagtgcatcc actcccggca tcgccgatct ggattcctgt
    10981 tgtaaaaacg ggatcgcctc cctcagccag ttatattcca cgctgtggtt aaccggtgct
    11041 aaaaacagcg tttccggcaa agacgttaaa aaataacgcg ccgaatgttg caaccgaaga
    11101 gtgccgcaga caatacaggt cgattcatta tcgacatgac tcaggcgatg agctgaattt
    11161 tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa tctctcccgt cggcatttcc
    11221 agcttcgccg ctccttgcgt taacgcatgc caacgaataa ccgataattc cccggcacca
    11281 tgtggaagct gccagtcact tcctaacacg caattcttat cgatcgttcc ttgtggagcg
    11341 ttaagcatca acaaacggct aagggcatcc atatcagact cctgagtggt ttttgattac
    11401 aaacaccaac aatcttagta aataactaat ctaataccat caaatggtta atcatgattt
    11461 ttgttttgtg attctgatta agaaaaagaa aataccagac gataagacaa aatatcaagc
    11521 gttgtgaaga aatggtattt gctcttttgc gtctatagtc atgatgtcaa atgaacgcgt
    11581 ttcgacagga aatcatcatg aataaatatc aggcagttat tattggtttt ggcaaggctg
    11641 gaaaaacatt agccgtcacg ctggcaaaag caggttggcg agtggctctc atcgaacaat
    11701 caaatgcaat gtatggcggg acctgtatta atatcggctg catcccaacc aaaacattgg
    11761 ttcatgacgc acagcagcac acagattttg tccgtgccat acagcgtaaa aatgaagtgg
    11821 ttaatttttt acgtaataag aattttcata atcttgcgga tatgcccaat atcgacgtga
    11881 tcgacggcca ggcggagttt atcaataatc atagcctgcg tgttcatcgg cctgagggaa
    11941 atctggaaat tcatggcgag aaaattttta ttaataccgg tgcacaaacc gtggttccgc
    12001 caattcctgg aattaccacc acgcctggtg tatatgacag caccggatta cttaatctaa
    12061 aagaattgcc tgggcattta ggtattttgg gcggcggata tattggcgtt gagttcgcct
    12121 ctatgttcgc taattttggc agcaaggtaa ccattttaga agcagcttcg ctgtttttgc
    12181 ctcgggaaga tcgggatatt gctgataata tcgcgacgat tttacgcgat cagggcgtcg
    12241 atattatcct caatgcccat gtggagcgaa tcagtcacaa tgaaaatcaa gtgcaagtgc
    12301 atagcgagca cgcccaactg gcggtggatg cactgttaat agcttccggt cgtcaaccgg
    12361 ctaccgcttc gttacatcca gaaaatgccg gtatcgcagt aaacgagcgc ggggcaattg
    12421 tcgttgacaa gcaattacat accaccgcag acaatatttg ggcgatggga gatgttaccg
    12481 gcgggctgca atttacttat atttcactgg atgattaccg cattgtgcgt gatgagttgc
    12541 tgggagaagg cagacgtagt actgatgatc ggaaaaatgt gccttattcc gtattcatga
    12601 caccgccact gtccagggtt ggtatgacag aagaacaggc cagagagagt ggtgctgata
    12661 ttcaggtggt gacattgcct gtagctgcaa ttccgcgtgc cagagtgatg aatgatactc
    12721 gtggggtatt aaaagcgatt gttgataata aaacccaacg tatattaggg gcatcactgc
    12781 tgtgtgttga ctcccacgag atgattaata tagtgaaaat ggtgatggat gccgggctgc
    12841 cttatagcat attacacgat cagatattta ctcatccgtc gatgagcgaa tcactcaatg
    12901 atctattttc attagtcaaa taaactcaaa atcagacgcc agaacaaata ttctggcgtc
    12961 tcagagaaaa gaatcttatt aattccctgt tactctataa cttcttaatc acttcattga
    13021 tggtatttta tatgttcaaa aaatctgttt tatttgcaac actattatct ggcgttatgg
    13081 cattttccac caatgcagat gataaaataa ttctgaaaca tatcagcgtc tcgtcagtat
    13141 cagcatcacc gacagttctg gaggatacca ttgctgatat agccagaaaa tataatgctt
    13201 catcctggaa agtcacatcg atgcgaattg ataataattc aaccgcaaca gcagtattgt
    13261 ataaataagg atgttcacaa tggaaaaata cctgcacctg ttaagtcggg gagataaaat
    13321 tggcctgaca ttgattcgtc tgagtattgc aattgttttt atgtggattg ggttattaaa
    13381 gtttgtccct tacgaggcag acagcattac accattcgtc gcaaacagtc cactaatgtc
    13441 gttcttttat gaacacccgg aagactataa acagtatctg actcacgaag gcgaatacaa
    13501 accagaagca agggcatggc aaacggccaa taatacctat ggtttttcca acggtcttgg
    13561 cgtcgtggag gtgattattg ctctgctggt tttggctaat cctgtcaatc gctggttagg
    13621 tttattggga ggactgatgg catttaccac gccgctggta acactctcat ttttaatcac
    13681 caccccggag gcatgggtac ccgcattggg tgacgctcat catggtttcc cttatttatc
    13741 cggtgctggt cgcctggtat tgaaagatac tctgatgctg gcaggtgctg tcatgataat
    13801 ggcagattcg gcgcgggaaa ttcttaaaca acgcagtaat gaatccagtt caacgttaaa
    13861 aactgaatat tgataatccg aacactgtct gttgttcact ttttcaggcg tcaggccccc
    13921 tttctgatga tgaagcctga cgccatatta ccggagatat gacagagtga agatcaatat
    13981 cactcccctg ttaaaagatt cttgtcgtct caggtaaaat atatgattaa tatcaatcat
    14041 gtatttttac agcaagacaa ccacgaacaa attcaggatt aaaatgtttt accgactccc
    14101 caacataacc caaatcaagc gaactaattt gtgccatttc tttctctgtc agtgagaaat
    14161 cccagatagc aaaattttct tcgatacgct gttgatgtgt cgatttcgga ataacgacca
    14221 ctccccgttg aatattccaa cgcagaatga cttgagaaat cgatttttga tgggcatctg
    14281 caatactctg aagcagatta ttttcaaagg gtttatgtcg tccaccacct aacggagccc
    14341 atgcttcagg ctgcacgtta taatacttca tggttgccag cgcctctggt tgggcaaaat
    14401 agggatgcaa ctcgacctgg ttgaccatcg gtttaactct taccgtttca caaaatttcg
    14461 ccagaacatg aggatagaaa ttggaaaccc caattgcttt taatttgccc tcttcatagg
    14521 catcttcgag tgcacgccac gcactgaaat aatcgcgcat agcctggtgc aataaataga
    14581 ggtcgaagta ctctagtccc gattttttta atgatgcttc aatacctgct gctgccgtat
    14641 cctgattcaa catatcctgc acccacaatt tagacgttat aaataactca tctcgggtac
    14701 ataaaccttc agatatagct tcacgaacgg cttcaccgac ggcatcttca ttaccgtata
    14761 ccgctgcagt gtcgatgagt cgataacctg tgcggatagc gttcaacact gactgtttgc
    14821 acacgttttt atcggtaacc tgaaaaacac caaatcccat catcggcatt ttcagattgt
    14881 tacttaatac ggaaaattcc actgtcagtc cctccattag gcatgaacaa tgagtctacg
    14941 ttaaaacgta acctcaaatt agtacgtgga ttttgatatc acttatgcaa aaaattcatt
    15001 aataatgcag gactgaaacc tctctatttt cggggacaac gaagcagacg ctaccagtgc
    15061 ttttgccttc gcccttgctg tttttgatac acttagggcc cagggcataa cgaaaatgtg
    15121 cgatatgacg aagaatcaat ggagaatgtg atgatcaggc agaagattct acagcagctc
    15181 ctggagtgga ttgagtgcaa tcttgagcac cctatttcaa tcgaagatat cgcacagaaa
    15241 tctggctaca gcagacgcaa catccagctt ctgttccgaa atttcatgca tgtgcctttg
    15301 ggagaataca ttcgcaaacg aaggctttgt cgtgccgcca ttcttgtccg gctcaccgcg
    15361 aaatctatgc ttgatattgc actctctttg cattttgatt cacagcagtc attcagccgc
    15421 gaatttaaaa agttattcgg ctgctctccc cgtgtatacc gccaccgtga ttattgggat
    15481 ctcgcaaata tcttcccttc ttttttaata cgtcaacagc aaaagaccga gtgtagatta
    15541 gtcaactttc ctgagacacc tatttttggc aattcattta aatatgatat tgaagtgtcg
    15601 aataaatcac cggatgaaga agtcaaactg cgacgtcatc atttagccag atgtatgaat
    15661 aattttaaga cggatatcta tttcgtctcc acgtttgaac cgtcaacaaa atcggtcgat
    15721 ttgctcacgg ttgaaacttt tgccggtacg gtatgcaaac aaaccgattc agagatgccg
    15781 aaagagtgga caattaaccg aggactgtat gcctctttcc gctatgaagg tgagtgggag
    15841 cactaccctg aatgggcgcg aaacctctat ctgatggaat tacccgccag ggggttagcc
    15901 agagtgaacg gcagtgatat tgagcggttt tattacaatg aaaatttcgt cgaaattgat
    15961 agcaataaca ttgtttgcga aatttttatc cccgttcgtc cggt
//
LOCUS       DAZGCS010000109        15803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGCS010000109 DAZGCS010000000
VERSION     DAZGCS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.111"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..162)
                     /gene="yciG"
                     /locus_tag="ACHK01_003887"
     CDS             complement(<1..162)
                     /gene="yciG"
                     /locus_tag="ACHK01_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8098760.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGN"
     gene            complement(543..1349)
                     /gene="trpA"
                     /locus_tag="ACHK01_003888"
     CDS             complement(543..1349)
                     /gene="trpA"
                     /locus_tag="ACHK01_003888"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO8098761.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1349..2542)
                     /gene="trpB"
                     /locus_tag="ACHK01_003889"
     CDS             complement(1349..2542)
                     /gene="trpB"
                     /locus_tag="ACHK01_003889"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO8098762.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     AREEI"
     gene            complement(2554..3915)
                     /gene="trpCF"
                     /locus_tag="ACHK01_003890"
     CDS             complement(2554..3915)
                     /gene="trpCF"
                     /locus_tag="ACHK01_003890"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO8098763.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3916..5511)
                     /gene="trpD"
                     /locus_tag="ACHK01_003891"
     CDS             complement(3916..5511)
                     /gene="trpD"
                     /locus_tag="ACHK01_003891"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO8098764.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5511..7073)
                     /gene="trpE"
                     /locus_tag="ACHK01_003892"
     CDS             complement(5511..7073)
                     /gene="trpE"
                     /locus_tag="ACHK01_003892"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO8098765.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7095..7188)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7165..7209)
                     /gene="trpL"
                     /locus_tag="ACHK01_003893"
     CDS             complement(7165..7209)
                     /gene="trpL"
                     /locus_tag="ACHK01_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO8098766.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7347..8228
                     /gene="rnm"
                     /locus_tag="ACHK01_003894"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             7347..8228
                     /gene="rnm"
                     /locus_tag="ACHK01_003894"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO8098767.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8225..8845
                     /gene="yciO"
                     /locus_tag="ACHK01_003895"
     CDS             8225..8845
                     /gene="yciO"
                     /locus_tag="ACHK01_003895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO8098768.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8946..9818
                     /gene="rluB"
                     /locus_tag="ACHK01_003896"
     CDS             8946..9818
                     /gene="rluB"
                     /locus_tag="ACHK01_003896"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO8098769.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9858..10448)
                     /gene="cobO"
                     /locus_tag="ACHK01_003897"
     CDS             complement(9858..10448)
                     /gene="cobO"
                     /locus_tag="ACHK01_003897"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO8098770.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10445..11203)
                     /gene="yciK"
                     /locus_tag="ACHK01_003898"
     CDS             complement(10445..11203)
                     /gene="yciK"
                     /locus_tag="ACHK01_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO8098771.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11423..12472
                     /gene="sohB"
                     /locus_tag="ACHK01_003899"
     CDS             11423..12472
                     /gene="sohB"
                     /locus_tag="ACHK01_003899"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO8098772.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12508..12759)
                     /gene="yciN"
                     /locus_tag="ACHK01_003900"
     CDS             complement(12508..12759)
                     /gene="yciN"
                     /locus_tag="ACHK01_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO8098773.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13139..15736
                     /gene="topA"
                     /locus_tag="ACHK01_003901"
     CDS             13139..15736
                     /gene="topA"
                     /locus_tag="ACHK01_003901"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO8098774.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGGALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3837 a         3993 c         4227 g         3746 t
ORIGIN      
        1 attaccaccg ctttgttgac cgcctttttt acccgcttca gatgcgcgtt gcggatcatt
       61 tttaaaatta ccgccgctat gctgaccgcc tttacggcct gcgtcggatg ccttctcacg
      121 gtcttcggcg aaatttcctg aaccaccacg atgttcggcc atgttatttc tcccgttgcg
      181 ttgcattgtt tcattaatat gagtgttgtg tgtcgacact cattaaaatt agtcgctaat
      241 gagaattagt caaattaagc gcaacgagaa gatagaggga aaatatattt tgaggaacat
      301 tctggatata ttaacaatta cctgaggaat aagtgactta gagaaaatta ttgattttac
      361 tggtgttatg ttgcgggatt aatttgtttg attatattac tgttgggcgg aaaaatgacg
      421 taagttgacg ttcgaccggg gtaagcgaaa cggtaaaaag ataaatatta aatgaattta
      481 ggatttttcc ggcttcatta aagaaagtta aaatgccgcc agcggaactg gcggctgtgg
      541 gattaactgc gcgtcgccgc tttcatcggt tgtacaaaaa ctttcagtgc cgccagcatt
      601 ttctctggct cattaatatg ttgctcgatg attttaacaa tggccgaacc agaaatcgcg
      661 cccgcagctc ctgcatcaat cgctgctttt acctgatccg gggcggaaat accaaacccc
      721 tgcaatggag gtgcagcgtt gtactctttc agcttcgcaa ccagatgatt gaggggtaac
      781 gcggcgcggt tttctgcgcc ggtcacgccc gctcgtgaca gcaaataggt gtaaccacga
      841 ccgtaagagg ctatctggcg cagcaggtcg tcatcggcat ttggcgggca gatgaagata
      901 ggtgcgacat tatggcgcaa cgcggcctgg cggaagggcg cggactcttc aattggcaca
      961 tcggcaacca gcaccgaatc gacgccgact ttctcgcact gggcataaaa ctcatcaatg
     1021 cctttgttaa acaccagatt ggcatacatc aacaggccaa tgggaatggt cgggtgtttc
     1081 tggcgaatca gtgccagcat ttcaaaacat tgtgccggag tcacacctgc cgcaaaggcg
     1141 cgcagagtgg cgttttgaat ggtcgggcca tccgctagag ggtcggagaa ggggatacct
     1201 aactccagcg cgtcagcacc ggcttcaatt agcgtatcga taattttcaa tgactgctca
     1261 atgcccggat caccgagcgt gacgaaagga acgaatgcgc cttctttgcg ctccttcaac
     1321 tgggcaaaca gagattcgta gcgttccatc agatttcctc tcgtgctttc aaaatatcgt
     1381 gaacggtgaa gatgtcttta tcgccgcgac cggaaaggtt aaccaccagt agctgctctt
     1441 tatccgggtt ttcgcgcatc attttcaacg catgggccag ggcgtgggag gattccagcg
     1501 ccgggatgat cccttcgtgc aggcacagcg ttttgaaggc ttcaagggct tcatcatcgg
     1561 taatagacac gtaatcagcg cgtccagtgc tgttaagata cgcgtgttgt gggccgacag
     1621 acgggaaatc cagtccggcg gagatggagt aagattcttc aatctgcccg tcttcggttt
     1681 gcatcatcgg cgctttcata ccgaaataga tgcccacgcg accatgtttt aacggtgcgc
     1741 cgtgctcgcc agtttcgata ccgtgaccac ctggctccac accaatcagg ccgacgttgg
     1801 tttcattgat gaaatcagca aacatgccga tggcattcga accgccgcca acacaggcga
     1861 taacggcatc cggcaggcga ccttctcttt ccagaatctg cgctttggtt tcttcgccaa
     1921 tcatccgctg aaactcacgc acaatggtcg gataaggatg cgggccagct gcggtgccca
     1981 gcatatagtg cgcggtttcg taactaccgg accagtcgcg cagcgcctcg ttacaggcat
     2041 ctttcagcgt cgcggaaccg ctatgcaccg ggatcacttc cgcacccatt aagcgcatac
     2101 gaaaaacgtt aggcgactgg cgttctacgt ctttggcacc catataaata cggcatttca
     2161 ggccgagcag ggcgctggca agggccgacg ccacgccatg ctgaccggca ccggtttcgg
     2221 cgatgatttc ggttttaccc atccgcttcg ccagtaaagc ctgaccgagc acctggttag
     2281 ttttatgcgc gccgccgtgc agcaaatctt cacgcttgag atacagagtg gtgttcgtcc
     2341 cggctgtaat gttctggcat ttggtcagcg cggttggacg cccggcatag tttttcagca
     2401 ggtcgttgaa ctgagcctga aattcaggat ctttttgcgc actgacaaaa gcttcttcca
     2461 gctggcgcag agcaggcatc aggatttgtg gcacgtacat gccgccaaac tcaccaaaat
     2521 aggggttaag taatgttgtc attgttcctt tccttaatat gcgcgcagcg tctggaaaac
     2581 cgaggccaaa agacgtgcgt ctttgatgcc cggttgcgac tctacagcag aattaaaatc
     2641 aagtccggcg cagccggttt gtgccgcttc cacgcagtta tctgcgccta agccccccgc
     2701 cagcagaacg ttgccaagcg attgaccatt taatagtgac cagtcgaaac gttgcccgct
     2761 tccaccctgg ccgttgtcta aaacatattt atcaacgtgc tgaagctcgc gggcgggcag
     2821 ggtttcaccg acgcttaatg ctttccagat ggcaacgtgt gctggcagag cttcacgcag
     2881 cgtatcgata tacagctgat cttcattacc atgcagttgc actgccgcca gcgataacac
     2941 cttagctttg tccaccacat cggcaatatc gtgattgcgg aacacgccaa catactgcaa
     3001 cggtgccgca gccatcactt cctgcgcctg ttcaacgttg acgcaacgcg gtgatgtcgc
     3061 aacaaaaatc aacccaccgt aaatcgcgcc cgcgtcataa gctgctttag cgtcttgccc
     3121 acgcgtcagg ccacatactt tattctcacc cagcaacacc cggcgcacgg cggcgtgcaa
     3181 atcgtcatgg gccatcaacg ccgaaccaat cagaaaaccg ttagcgaagt ggcttaactc
     3241 gcgcacctga gcgtaagtat tgatgccgga ttcgctgatt accgttacgt tgtgccccag
     3301 tttcggcgca agctcgcggg tacggttgag atcaatcgac aaatcacgca gatcgcggtt
     3361 gttgatgcca acgacctttg cccccaatgc aatggcacgc tccagttcct cttcattact
     3421 gacttcggtc agcacaccca tctccagact gtgggcgacg gcggcaagct ggcgatattg
     3481 ttcgtcatcc agtactgaaa gcattaataa gcaggcatca gcctggtaat agcgcgccag
     3541 atagatctgg taaggatcga taatgaagtc tttacataaa atcggctgcg gggcgatttg
     3601 gctgacgatg gggaggaaat caaagctccc ctgaaaatat ttctcatcag tcagcaccga
     3661 aattgccgaa gcgtaatgtt tataaatggc ggcaatgcgt gctggatcga aatcatcacg
     3721 gatcacgcct tttgacggcg acgctttctt gcactccaga ataaacgccg tgcgcgcacc
     3781 ctgtagcgca tcataaaaat gtcgcgtgct cggctgaacc tcattctgaa aactggccag
     3841 cggttgctgc tgtttgcggg tttctaccca aatcgccttg tctgcgacga ttttcgctaa
     3901 aacggtttgc atcatttacc ctcgtgccgc cagtgcggta actctgtcgt aagcggaacc
     3961 actgcgcagt acctcaagaa cggtttgcgc attggcttgc agatcttcat ggccatgcag
     4021 gcgcattaac atggcgacgt tcgcagcgac ggctgcttca tgggcggcgt cgcctttacc
     4081 ttgtaacaag cgtgttaaaa tgtcacggtt ttcttccggt gttccgcctg ccagttgctc
     4141 ctggtggtag ggagtcaggc caaaatcttc agcggtcaat tgatagctct taatttcgcc
     4201 gtcatgcagc tcggcaacga ttgtcggcgc gtgtaatgaa acttcatcca tcccgccgct
     4261 gtgcaccacc gccgcgcgtt gataccccag cacgcgcaag gtttcggcaa tcggcagcac
     4321 cagttccgga ctataaacac caattaacgc cagcggcgga tgcgccgggt taatcaatgg
     4381 ccccagcaca ttgaacaggg tgcgggtttt cagttgctgg cgaaccggca tcgcgtggcg
     4441 gaatccggtg tgatacttcg gcgcaaagag gaaacataca cctaactcat ccagcgcctg
     4501 gcgcgattta tcggcgttca tatcaagatt aataccgaac gccgccagca gatcggacga
     4561 accagattta ctggagacgc tacggttgcc gtgtttcgcc actttcagcc cacaggccgc
     4621 ggcgacaaac gcactggcgg tagaaatatt gatactgttg ctgccgtcac cgccagtacc
     4681 gacgatatca gcaaacagat aatccgggcg cgggaacggc gcggcgtttt ccagtagcgc
     4741 ggttgctgct ccggcgatct cgttcgggtg ctcaccgcga attttcatgc tcaccagcgc
     4801 cgccgccagt tgttccggct tcagctcgcc acgcaccacc gctgaaaaca gctggtggct
     4861 ttcttgttgg ctaagcgtct gcgcctgata cagtttttcc agaatcggtt gcagcgtgtt
     4921 ggctggctct agtttctgct gcgcccaggc cagcgtttgt tccagcaggc gagcgccctg
     4981 ggtggtgaga atggattccg gatggaactg gaatccacaa acgcgatccg catcgtgacg
     5041 tactgccatc accatgccat taaaatgggc gttgatggtt aaaccggccg gaatgttact
     5101 gccaaccagc gagtgataac gcgccaccgg cagcgggttt gttaatccgg caaacatcgc
     5161 ctgaccgtca tgttcaatgc tcgacgcttt accgtgaaga atttcgcccg cctgaccgac
     5221 atagcccccg taagcttcga caattgcctg atgtccgagg caaatgccaa taatgggcag
     5281 cttgccacgc aagcgggtga ggagttccgg catacaaccg gcttcgctcg gcacaccggg
     5341 gccaggagaa agcatcagta ccggattgct catcgtcgcc aggcgttcaa ttaaggtctg
     5401 cgccggaata tggttgcggt aaatcaccac attatgccca ttgctgcgca actgatctgc
     5461 caggttgtac gtaaaagagt cgatattatc gagcagcaga atgtcagcca tcagaaagtc
     5521 tcctgtgcat gatgcgcggt ggcaatagcg cgcagtacag cgcgggcttt attacgggtt
     5581 tcgtcggctt ccgactgcgg aacagaatca aggactacgc cagcaccggc ttgcacggtg
     5641 gcgataccgt tttccaccag cgccgagcgg atcacaatgc aggtgtcgag atcgccatgc
     5701 gcggtgaaat aacctaccgc gccgccgtag ctgccgcggc ggcgaccttc cgcctcggca
     5761 attaactgca tagcgcgtac tttcggcgca ccacttaacg ttcccatatt catacaggcg
     5821 cggtaagcgt gcagggcgtc gagatcgtgg cgcagctcac cgaccacgcg ggagaccaag
     5881 tgcatcacgt aagagtaacg gtcaactttg gtgaggtcgg cgacgtagcg gctgccgggg
     5941 gtgcaaatgc gtgccagatc attacgggcg agatccacca gcatcagatg ttcagaaagc
     6001 tctttatgat cggtacgcat ttccagttcg atgcggctgt cgaggtctct gtccagcgag
     6061 ccatcggcac gacgaccgcg tggacgtgtt ccggcaatcg ggtagatctc aatctggcgg
     6121 ctggtggcgt catacttgag cgaactttcc ggcgacgcgc caaacagggt gaaatcatta
     6181 tcctgcataa aaaacatgta cgggctggga ttactctttt tcagcacgta ataggccgcc
     6241 agcggtgacg ggcagggcag agagaaacgg cgagatggca ccacctggaa aatttctcca
     6301 gcgcgaatcg ctttttgcaa caaacgcact acgccaccga actcttcatc gctctgatta
     6361 cattcacaac gcatatgcgg cacggaaacc actggcagcg gcggcgcggc ttcggtcagt
     6421 tgctgacgta gttcgttcag gcgagcagtg agacgttgtt tttcttcttc attcggagca
     6481 aacaggctgg cctgaatacg ggtgcttttt ttctgatggt caatcaccat cagagtttca
     6541 gcgagataaa aacagaaatc agggcagtta ttttccgctg acagttgcgg taaatcttca
     6601 aatcccgcca caaggtcata agagaacagg ccgccgaaga acattgcttc tcgttcttcc
     6661 ttcggtacat tcaacagatt ctgcaataaa cggaaagcgt caaaaaccga aagggagcat
     6721 aagcgagcgt cttcatccag cagtggactg acagggggga agcgcagcac acggcagttt
     6781 ggtaattgtt cattttccac acccgcaggc agggcgttat ccagtagtgt cagcagggct
     6841 tcgccgttgc cggaaagtgc ctggattgtg acagtgtcac ctaaagctgt aatgcgcagc
     6901 gcactgtcta ccagcagcag gctttttaaa tcatctttgc tgtcgatatc tgcggattcc
     6961 agcagcagcg ttgccggacg atccccacac aactggtgaa aaagcgcggt gggattgtcg
     7021 cgataagcgc cttcgcaggt tagcagttcg agagtcggtt tttgtgtttg cattgttatt
     7081 ctctaatttt gttcaaaaaa aagcccgctc attaggcggg ctgggtatct gattgcttta
     7141 cgcatggtga atacactgcc cgtttcagga agtgcgccac caacctttca gtacgaaaat
     7201 tgctttcatt gtcgataccc tttttacgtg aacttgcgta ctagttaact agttcgatga
     7261 ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa
     7321 aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg atttacgacc
     7381 tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg
     7441 cagtcgagat gcgcgtcggc accctggcga tcaccgacca tgacaccaca gctgcaatcg
     7501 cgccagcaag agaggaaatt tcacgttcgg gactggcgtt gaatcttatt cccggcgtgg
     7561 aaatttccac ggtctgggaa aatcatgaaa ttcatattgt cgggctgaat attgatatta
     7621 ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga acggcgcaat cagcgggcgc
     7681 agctgattgc cgaaagactt gaaaaagcgc aaatacctgg ggcgctggaa ggcgcacaac
     7741 gactggcgca gggcggcgca gtgacgcgcg gtcattttgc gcgtttcctt gttgagtgcg
     7801 gcaaagccag ttcaatggcg gatgtcttta aaaagtatct ggcgcgcggg aaaaccggat
     7861 acgttccgcc acagtggtgt acaatagaac aagctattga tgtcattcat cattctggcg
     7921 gtaaggcggt gctagctcat cctgggcggt acaatcttag tgctaaatgg ctgaaaagat
     7981 tggttgcgca ttttgccgaa caccacggtg acgcgatgga agtcgcgcag tgccagcaat
     8041 cgcccaatga acgtacccag ctggcggccc ttgcgcgtca gcatcattta tgggcatcac
     8101 aaggatctga ttttcatcag ccatgcccgt ggatcgaact gggtcgtaaa ctctggctgc
     8161 ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc gcagaacacc acagagaggg
     8221 aattatgagc cagttttttt atattcatcc tgataaccca cagcaacgtc tgatcaacca
     8281 ggcggtggag atcgtgcgta aaggcggggt gattgtttat ccaactgatt ccggctatgc
     8341 gctcggctgt aaaattgaag ataaaaacgc gatggagcgt atttgtcgta ttcgccagct
     8401 gccggacggt cacaacttta ccctgatgtg tcgcgatctt tctgaactct cgacctattc
     8461 atttgttgat aacgttgcgt ttcgtttaat gaaaaacaac acgccgggca actatacctt
     8521 catcttgaag gggacgaagg aagtgccacg ccgtctgttg caggaaaaac gcaaaaccat
     8581 cggtatgcgt gtgccttcta acccgattgc gcaggcgttg cttgaagcac tgggcgaacc
     8641 gatgctttcc acttcgctaa tgctgccagg cagcgaattt accgaatccg atcccgaaga
     8701 aattaaagac cgtctggaaa aacaggtgga tttgattatc cacggcggct atctcggaca
     8761 gaaaccgaca acggttattg atcttaccga tgatacgccg gtcgtggtgc gtgaaggcgt
     8821 aggtgatgtg aagcctttct tataagtgcg taactctgta tactacgcgg ccttaaaatg
     8881 gcgctatacg gcgccattga ttctcctgtt cgacgcctgt gaaggcgaca ccaaaaggaa
     8941 gctctatgag cgaaaaatta cagaaagtgc tggcccgcgc tggccacggc tcccgccgtg
     9001 aaatcgaatc tattattgaa gccggtcgtg tgagtgttga tggcaaaatt gctaaactcg
     9061 gcgatcgcgt tgaagttacc cctgggctga aaattcgtat cgatggtcat ctgatttcgg
     9121 tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta ttacaaaccg gaaggtgagt
     9181 tgtgtacccg taacgatccg gaagggcgtc cgacggtgtt tgaccgtctg ccaaagctgc
     9241 gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa cacctgtggt ctgctgctgt
     9301 tcaccaccga tggtgaactg gcaaaccgtt taatgcatcc aagccgtgaa gttgaacgtg
     9361 aatacgccgt gcgtgtattt ggtcaggttg acgacgcgaa actgcgtgat ttgagtcgtg
     9421 gcgtgcaact ggaagatggt ccggcagctt ttaaaaccat caagttcagc ggcggcgaag
     9481 ggatcaacca gtggtacaac gtgactctga ccgaaggccg taaccgtgaa gttcgtcgtc
     9541 tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg tgttcgttac ggtgatatcc
     9601 cactgccaaa aggtctgcca cgcggtggct ggacggagct ggacctcgcc cagactaact
     9661 atctgcgcga actggtggag cttccgccag aaaccagctc taaagtcgct gtagaaaaag
     9721 accgtcgtcg catgaaggct aatcagattc gtcgtgcggt gaaacgtcac agtcaggtga
     9781 gcggcggtcg tcgttctggc gggcgtaata acggttaatc aaagtatgcc ggacgtcata
     9841 tccggcattt ttacagatta ataatcaatc cctatttgcg ccttaacacc ggcatcaaac
     9901 gcatgtttga tggggcgcag ctcacttacc gtatccgcca gttcaagaat atcccgatga
     9961 cagcctcgac ccgtgataat caccgtctgt tgatgtggac gttcatttaa cgcctgcacc
    10021 acttcttcca gtggcaaata gtcatacgcc accatatacg tcagttcatc aagcaaaacc
    10081 atatccagtg aggaatcagc aagcatccgc tttgcatgtt gccagacttc gcggcaggcg
    10141 gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc ccgttgccat cacctgaaac
    10201 tcaacgccat gtggctccag cagattgcgt tcgccattag gccaggtgcc tttaataaac
    10261 tgcacgacgc ctactttttt tccgtgacca actgcgcgtg ttgccgtacc aaatgccgcg
    10321 gtggttttgc cttttccatt gccggtaaag acgatgataa taccgcgttc atcctgggcc
    10381 tgggccacac gagcatctac tttttctttc actcgctgct gacgctgttg gtagcgttca
    10441 tcactcattg ggaaattcct ggtttacggc ccggttgggc gtcaaaggtc atgccggttt
    10501 tacggcggct gtcatcgccc atcagccaga ggtagagcgg catgatatca gcgggtgttt
    10561 taagtttctg tggatcttcg gtcgggaagg cgctggcacg cattgcggtg cgcgtaccgc
    10621 ctgggttaat gcagttgacg cgcaggcgct gctgatattc atcggccagt acctgcatca
    10681 tcccttcggt ggcaaatttc gacgctgcat atgcgcccca gttggctcgt ccctgacgtc
    10741 caacgcttga tgaagtaaag accagtgaac cggcgtccga tttgagtaat aaaggaagca
    10801 gtgcctgggt gagcataaag gtggcattaa cgttgacctg tatgacgtcc tgccagacct
    10861 gtggattttg ttcgctcatt gggcaaacat cgccgagcaa tccggcatta tgcaaaacac
    10921 catccagacg cggataatta acggtaatgc gctgtgccag ttgttggcaa tcttcggacg
    10981 tgcaggtcag caaatcgaga ataaaccact gtggctgacg tccagtttct tcgtttatgt
    11041 ggctggctac ctgacgtaat ttttcttcat tacggcccaa cagaatcact gtcgcaccat
    11101 agcgtgcata cgtcatcgcg gcttcacggc caatcccatc gctggctccc gtcaccagga
    11161 taatgcgatc attgagtaaa tcttgttttg gctggtaatg catggctact cctcaacgac
    11221 gttgtctgtc gtgctgccag tgtactttat gactttgggg ctttatgcct gaaacagaag
    11281 ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg atactttgtc atactttcgc
    11341 tgcaataaca tctctgcgag acggcttaac atgcctgttg taaactgtga gccaaagcgt
    11401 tgtttaacca aggtggggac tcgtggaatt gttgtctgaa tatggtttgt ttttggcgaa
    11461 aatcgttacc gttgtgctag cgattgcggc gattgccgcc attattgtca atgttgctca
    11521 acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat ctcagcgaac agtataagga
    11581 gatgaaagaa gaactggccg cggcgctgat ggactcacat cagcaaaaac agtggcacaa
    11641 agcgcagaag aaaaagcaca agcaagaagc gaaagcagca aaagcgaaag ccaaactggg
    11701 cgaggtggca actgacagta aacctcgcgt ctgggtgctg gattttaaag gcagcatgga
    11761 cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg gtactcgcag cattcaaacc
    11821 gcaggatcag gttgtgctcc gtctggaaag ccctggtggc atggtgcatg gttacgggtt
    11881 ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt cctttaactg ttacggtaga
    11941 caaagtcgct gccagcggcg gttacatgat ggcctgtgtg gcggacaaaa ttgtttccgc
    12001 accgtttgct attgtgggtt ccattggggt ggtggcgcaa atgcccaact ttaaccgctt
    12061 cctgaaaagc aaagatattg atatcgaact gcacactgcc gggcagtata agcgtactct
    12121 gacgttgctg ggtgaaaata ccgaagaagg gcgggagaaa ttccgcgaag agttgaacga
    12181 aacgcatcag ttgtttaaag attttgtgaa gcgtatgcgt ccgtctctgg atattgaaca
    12241 ggtggcaacg ggtgaacact ggtacggaca acaggcggta gagaaaggcc tggttgatga
    12301 aatcaacacc agtgatgaag ttattcttag cctgatggaa ggccgtgaag tggttaatgt
    12361 acgctatatg cagcgtaaac gactcattga ccgattcacc ggcagcgcgg cagagagcgc
    12421 cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag ccattgatgt aaaagacaaa
    12481 cgcgaggcta agacctcgcg ttttgcttta atcaaccaga tgatattttt ctgaaagcac
    12541 atgggccagg tgtttgaaca tattaaacac cgcggtgctt ttggctgttg gcaatccttg
    12601 ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca ttacgctgcg tgacgccggt
    12661 ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg attttgttgg cctctttcaa
    12721 cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt atttactcct taaacaagga
    12781 cattagtcta cgccaggcat ggcttgcaga caaatatacc acgctggtgg caagagcgcc
    12841 ttactggtaa ctttggattt tgcatgctaa taaagttgcg tatcggattt tatcaggtac
    12901 agtgtgacgc tttcgtcaat ctggcaatag atttgcttga cattcgacca aaattccgtc
    12961 gtgctatagc gcctgtaggc caagacctgt taactcagtc acctgaattt tcgtgaacag
    13021 agtcacgaca aggggttgat atccgcagag aacgagtcca tatcggtaac tcgttgccag
    13081 tggaaggttt atcaacgtgc gacgcattcc tggaagaatc aaattaggta aggtgaatat
    13141 gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc aaaacgatca acaagtatct
    13201 gggtagtgac tacgtggtga aatccagcgt cggtcacatc cgcgatttgc cgaccagtgg
    13261 ctcagctgcc aaaaagagtg ccgactctac ctccaccaag acggctaaaa agcctaaaaa
    13321 ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac ccgtggcaca attgggaggc
    13381 gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct gaactgaaac aactggctga
    13441 aaaagccgac cacatctatc tcgcaaccga ccttgaccgc gaaggggaag ccattgcatg
    13501 gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat agccgagtgg tgtttaacga
    13561 aattactaaa aacgcgatcc gccaggcatt taacaaaccg ggtgagctga atattgatcg
    13621 tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg gttgggtata tggtttcgcc
    13681 gctgctatgg aaaaagatcg ctcgtggtct gtctgccggt cgtgtgcagt cggtggcggt
    13741 tcgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc gtgccggaag agttctggga
    13801 agtcgatgcc agcacgacca cgccatctgg tggagcgttg gcgttgcagg tgactcatca
    13861 gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact caggctgcgg taagtctgct
    13921 ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa ccgacaacca gtaaacctgg
    13981 cgctcctttt attacctcta cgctgcaaca agctgccagc acccgtcttg gatttggcgt
    14041 gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca ggctatatca cttacatgcg
    14101 taccgactcc actaacctga gtcaggacgc ggtaaatatg gttcgcggtt atatcagcga
    14161 taattttggt aagaaatatc tgccggaaag tccgaatcag tacgccagca aagaaaactc
    14221 acaggaagcg catgaagcga ttcgtccttc tgacgtcaat gtgatggcgg aatcgctgaa
    14281 ggatatggaa gcagatgcgc agaaactgta ccagttaatc tggcgtcagt tcgtagcctg
    14341 ccagatgacc ccagcgaaat atgactccac gacgctgacc gttggtgcgg gcgatttccg
    14401 cctgaaagca cgcggtcgta ttttgcgttt tgatggctgg acaaaagtga tgcctgcgtt
    14461 gcgtaaaggc gatgaagatc gcatcttacc agcagttaat aaaggcgatg ctctgacgct
    14521 cgttgaactt acaccagccc agcactttac caagccgcca gcccgtttca gtgaagcatc
    14581 gctggttaaa gagctggaaa aacgtggtat cggtcgtccg tctacctatg cgtcgatcat
    14641 ttcgaccatt caggatcgtg gctacgtgcg agtagaaaat cgtcgtttct atgcggaaaa
    14701 aatgggcgaa atcgtcaccg atcgccttga agaaaatttc cgcgagttaa tgaactacga
    14761 ctttaccgcg cagatggaaa acagcctcga ccaggtggca aatcacgaag cagagtggaa
    14821 agctgtactg gatcacttct tctcggattt cacccagcag ttagataaag ctgaaaaaga
    14881 tccggaagag ggtggtatgc gcccgaacca gatggttctg accagcattg actgcccgac
    14941 ttgtggtcgc aaaatgggga ttcgcacagc gagcaccggg gtattccttg gctgttctgg
    15001 ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt aacctggtgc cggaaaacga
    15061 agtgctgaac gtgctggaag gcgaagatgc tgaaaccaac gcgctgcgcg caaaacgtcg
    15121 ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc gatccgaaac gtaagttgca
    15181 tgtctgtggt aataacccaa cctgcgacgg ttacgagatc gaagagggcg aattccgcat
    15241 taaaggttat gacggcccga tcgttgagtg tgaaaaatgt ggctctgaaa tgcacctgaa
    15301 aatggggcgt ttcggtaaat acatggcctg caccaacgaa gagtgtaaaa acacacgtaa
    15361 gattttacgt aacggcgaag tggcaccacc gaaagaagat ccggtgccat tacctgagct
    15421 gccgtgcgaa aaatcagatg cttatttcgt gctgcgtgac ggtgctgccg gtgtgttcct
    15481 ggctgccaac actttcccga aatcgcgtga aacgcgtgcg ccactggtgg aagagcttta
    15541 tcgcttccgc gaccgtctgc cggaaaaact gcgttatctg gccgatgcgc cacagcagga
    15601 tccggaaggt aataagacca tggttcgctt tagccgtaaa accaaacagc aatatgtctc
    15661 ttcggaaaaa gacggaaagg cgactggctg gtcagcattt tatgttgatg gcaaatgggt
    15721 tgaaggaaaa aaataacctt taattctgtc aggtttttat aaacaaaggg tcgcgaaagc
    15781 ggcccttttt tattgcatat tat
//
LOCUS       DAZGCS010000110        15583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGCS010000110 DAZGCS010000000
VERSION     DAZGCS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.112"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1315)
                     /gene="icd"
                     /locus_tag="ACHK01_003902"
     CDS             complement(65..1315)
                     /gene="icd"
                     /locus_tag="ACHK01_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO8098775.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1487..2140
                     /gene="rluE"
                     /locus_tag="ACHK01_003903"
     CDS             1487..2140
                     /gene="rluE"
                     /locus_tag="ACHK01_003903"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO8098776.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2150..2611
                     /gene="nudJ"
                     /locus_tag="ACHK01_003904"
     CDS             2150..2611
                     /gene="nudJ"
                     /locus_tag="ACHK01_003904"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO8098777.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2665..3771
                     /gene="mnmA"
                     /locus_tag="ACHK01_003905"
                     /gene_synonym="trmU"
     CDS             2665..3771
                     /gene="mnmA"
                     /locus_tag="ACHK01_003905"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO8098778.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3807..4448
                     /gene="hflD"
                     /locus_tag="ACHK01_003906"
     CDS             3807..4448
                     /gene="hflD"
                     /locus_tag="ACHK01_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO8098779.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4452..5822
                     /gene="purB"
                     /locus_tag="ACHK01_003907"
     CDS             4452..5822
                     /gene="purB"
                     /locus_tag="ACHK01_003907"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO8098780.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5990..6661
                     /gene="phoP"
                     /locus_tag="ACHK01_003908"
     CDS             5990..6661
                     /gene="phoP"
                     /locus_tag="ACHK01_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO8098781.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6661..8121
                     /gene="phoQ"
                     /locus_tag="ACHK01_003909"
     CDS             6661..8121
                     /gene="phoQ"
                     /locus_tag="ACHK01_003909"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO8098782.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8197..9318
                     /gene="roxA"
                     /locus_tag="ACHK01_003910"
                     /gene_synonym="ycfD"
     CDS             8197..9318
                     /gene="roxA"
                     /locus_tag="ACHK01_003910"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO8098783.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9464..10693)
                     /gene="pepT"
                     /locus_tag="ACHK01_003911"
     CDS             complement(9464..10693)
                     /gene="pepT"
                     /locus_tag="ACHK01_003911"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO8098784.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCNIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10943..12079
                     /gene="potA"
                     /locus_tag="ACHK01_003912"
     CDS             10943..12079
                     /gene="potA"
                     /locus_tag="ACHK01_003912"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO8098785.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12063..12926
                     /gene="potB"
                     /locus_tag="ACHK01_003913"
     CDS             12063..12926
                     /gene="potB"
                     /locus_tag="ACHK01_003913"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO8098786.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            13289..14650
                     /gene="espK"
                     /locus_tag="ACHK01_003914"
     CDS             13289..14650
                     /gene="espK"
                     /locus_tag="ACHK01_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO8098787.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(15027..>15583)
                     /locus_tag="ACHK01_003915"
     CDS             complement(15027..>15583)
                     /locus_tag="ACHK01_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO8098788.1"
                     /translation="DKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPE
                     HSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEY
                     MAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAP
                     VEPLAIIDRTFGMKENISMVNASK"
BASE COUNT         4020 a         3869 c         3761 g         3933 t
ORIGIN      
        1 tacctacata aataaaacaa cgggcgtgtt atacgcccgt tgcaatatta aacacatgta
       61 gtgattacat gttcttgatg atcgcatcac caaattctga acatttcagc agtttagcgc
      121 cttccatcag acgctcgaag tcataggtta cggtcttcgc attgattgcg ccttccatac
      181 ctttaacaat caggtctgcg gcttcagtcc agcccatatg gcgtaacatc atctcagcgg
      241 agagaataat agagccaggg tttactttgt cctgaccggc gtatttcggc gcagtaccgt
      301 gggtggcttc aaacagggcg cattcgtcac cgatgtttgc accaggggcg ataccgatac
      361 cgccaacctg cgctgccagg gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga
      421 taacatcata ttcagccgga cgcagcagga tctgttgcag gaatgcatca gcaatcacgt
      481 ctttaatgac gatctctttg ccggtgttcg ggtttttaac tttcagccac gggccgccgt
      541 cgatcagttc accgccaaac tcttcacgcg ccagctggta gccccagtct ttaaacgcgc
      601 cttcggtgaa cttcatgatg ttgcctttgt gcaccagggt cacagagtca cggtcgttgg
      661 caattgcgta ttcaatcgcg gcacggacca gacgtttggt gccttcttca gaacacggct
      721 tgataccgat accgcaatgt tccgggaagc gaattttctt cacgcccatc tcttcacgca
      781 ggaatttaat cactttctcg gcgtcggcag agtctgcttt ccattcgata cccgcataaa
      841 tgtcttccga gttttcacgg aagataacca tatcggtcag ttcagggtgt ttaaccgggc
      901 ttggagtgcc ttgatagtaa cgtaccggac gcaggcagat gtagagatcc agttcctggc
      961 gcagggcaac gttcagagag cgaataccgc caccaacagg agtggtcagc ggacctttaa
     1021 tagcaacgcg atattcacga atcagatcaa gggtttcagc aggcagccag acatcctgac
     1081 cataaacctg tgtggatttt tcaccggtgt aaatttccat ccaggagatt ttacgctcgc
     1141 ctttataggc tttctcgact gcagcgtcga ccactttcag catggctggg gttacatcta
     1201 caccgattcc atcaccttca atgtaaggga taatcggatt ttcaggaacg ttgagtttgc
     1261 cgttttgcag ggtgatcttc ttgccttgtg ccggaacaac tactttactt tccattcacc
     1321 tctccttcga gcgctactgg tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc
     1381 tgcgatacgg atgctttaga gcaatttttt gttaatgatt tgtaattggc ttgtcaatac
     1441 taccctattg tttgccgtca tgaaagatgc gcgttattag gctataatgc ggcaattcat
     1501 aatctctgaa aataccatgc aaaaaacttc ttttagaaat caccaggtta agcgattcag
     1561 ctcgcaacgt tctaccaggc gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa
     1621 taaaccctac gatgttcttc cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga
     1681 attcatcccg gttcagggcg tttatgcagc aggtcgtctt gaccgcgata gcgaagggtt
     1741 actggtgctg accaataacg gcgcgttgca ggcgcgttta acccagccgg gtaaacgcac
     1801 cgggaaaatc tattatgtgc aggtggaagg tattcccaca caagacgcac ttgaagcctt
     1861 gcgcaatggc gtaaccttaa atgatggccc taccctgccc gccggcgcgg agctggttga
     1921 cgaaccagcg tggttatggc cgcggaatcc accaattcgt gaacgcaaaa gtattcccac
     1981 cagctggctg aagatcacct tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc
     2041 ccatgttggt ttccccacgc tgcgactgat tcgctatgcg atgggagatt actctttgga
     2101 taatcttgcc aatggcgaat ggcgagaagt gacagattaa ggatgcaaaa tgtttaaacc
     2161 gcacgttacc gttgcttgcg tggtgcacgc agaaggcaaa tttttagtcg ttgaagagac
     2221 gattaatggt aaagcgttat ggaaccaacc tgccgggcat ctggaagccg atgaaacctt
     2281 agtggaagcc gccgcccgtg aactgtggga agaaaccggc atcagcgcgc agccgcaaca
     2341 ctttattcgt atgcatcagt ggattgcgcc agataaaacg ccgtttttgc gtttcctctt
     2401 tgccattgag cttgagcaaa tatgcccgac ccagcctcat gacagcgata tcgactgctg
     2461 tcgttgggtc agcgccgaag agattttaaa ggcgtcaaat cttcgttcgc cgctggtggc
     2521 ggaaagtatc cgttgttatc aaagcgggca acgctatccg ctggagatga ttggcgattt
     2581 taactggcct tttacaaagg gtgtcatcta aaaggctgcg tgatagaata cgccgccttg
     2641 aagttcaatg tcgtgagtga tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg
     2701 tccggcggtg tcgattcctc cgtttctgcc tggctgttgc aacaacaggg atatcaggtt
     2761 gaaggcctgt ttatgaagaa ctgggaagaa gacgacggtg aggaatattg cacagcggca
     2821 gcggatctgg ctgatgccca ggctgtctgc gacaagctcg gcattgaact gcacaccgtt
     2881 aactttgctg ccgagtactg ggacaacgtc ttcgaactgt tccttgccga atataaagcc
     2941 ggtcgcacgc cgaatccgga tattctgtgc aacaaagaga tcaaatttaa agccttcctc
     3001 gaatttgccg ccgaagattt aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg
     3061 gccgatgtcg atggcaagag ccgcctgctg cgtggtctgg acagcaataa agaccagagc
     3121 tacttccttt atacgctcag ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa
     3181 ctggaaaaac cgcaggtgcg taagattgct gaagatcttg gtctggtcac cgcgaagaaa
     3241 aaagactcta ccggcatttg cttcattggc gaacgtaaat tccgcgagtt cctgggccgt
     3301 tatctcccgg cgcaaccggg caaaatcatt accgtcgatg gcgatgaaat tggtgagcac
     3361 caggggctga tgtatcacac tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa
     3421 gaaggtaccg aagaaccgtg gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt
     3481 gtcgctcagg gccatgaaca cccgcggctg atgtctgtcg ggttgattgc ccagcagttg
     3541 cactgggtcg atcgcgaacc attcaccggc actatgcgtt gcacggtaaa aacccgctat
     3601 cgccagaccg atatcccttg caccgtcaag gcgctggacg atgatcgcat tgaagtgatt
     3661 ttcgatgaac cggttgccgc cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa
     3721 gtgtgcctcg gtggcggtat tattgagcag cgtctgccgc tgccggtctg attattatct
     3781 ttacttaaaa caaggaagca gtgaacgtgg caaagaatta ctatgacatc accctcgccc
     3841 tggccggtat ttgtcagtcg gcacgcctag tgcaacaact cgctcaccag gggcattgtg
     3901 atgccgatgc gctacacgtc tcactcaaca gtattattga tatgaacccc agctcgacgc
     3961 tggcggtttt tggcggtagc gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg
     4021 tgctcaatgc cagcagtcgc cagggcttaa acgccgaatt aacccgctac acactcagct
     4081 tgatggtgct tgagcgcaaa ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc
     4141 ggatcaacgg cctgcaacgc cagctcgaac acttcgattt acagtccgaa acgctgatga
     4201 gcgcgatggc tgctatctat gttgatgtga tcagcccgct tggcccgcgc attcaggtca
     4261 ccggttcccc tgctgtactg caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc
     4321 tggcaggcat tcgcgccgcc gtgctctggc accaggtcgg cggcggacgt ctgcaactga
     4381 tgttttctcg taatcgcctg accactcagg caaaacaaat tcttgctcat ttaaccccgg
     4441 agttgtgatc tatggaatta tcctcactga ccgccgtttc ccctgtcgat ggacgctacg
     4501 gcgataaagt cagcgcgctg cgcgggattt tcagcgaata tggtttgctg aaattccgtg
     4561 tacaagttga agtacgttgg ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc
     4621 ctgcttttgc tgccgacgca atcggttacc ttgatgcaat tgtcgccaat ttcagcgaag
     4681 aagatgccgc acgcatcaaa accatcgagc gtaccactaa ccacgacgtt aaagcggttg
     4741 agtatttcct gaaagaaaaa gtggcggaga tcccggaact gcacgcggtt tctgaattca
     4801 tccactttgc ctgtacttcg gaagatatca ataacctctc ccacgcatta atgctgaaaa
     4861 ccgcgcgtga tgaagtgatc ctgccgtact ggcgtcaact gattgatggc attaaagatc
     4921 tcgccgttca gtaccgcgat atcccgctgc tgtctcgtac ccacggtcag ccagccacgc
     4981 cgtcaaccat cggtaaagag atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc
     5041 agcttaatca ggtggagatc ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc
     5101 atatcgccgc ttacccggaa gttgactggc atcagttcag cgaagagttc gtcacctcgc
     5161 tgggtattca gtggaacccg tacaccaccc agatcgaacc gcacgactac attgccgaat
     5221 tgtttgattg cgttgcgcgc ttcaacacca ttctgatcga ctttgaccgt gacgtctggg
     5281 gttatatcgc ccttaaccac ttcaaacaga aaaccattgc tggtgagatt ggttcttcca
     5341 ccatgccgca taaagttaac ccgattgact tcgaaaactc cgaagggaat ctgggccttt
     5401 ccaacgcggt attgcagcat ctggcaagca aactgccggt ttcccgctgg cagcgtgacc
     5461 tgaccgactc caccgtgctg cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt
     5521 atcaatccac cctgaaaggc gtgagcaaac tggaagtaaa ccgtgaccat ctgctggatg
     5581 agctggatca caactgggaa gtgctggctg agccaatcca gacagttatg cgtcgctatg
     5641 gcatcgaaaa accgtacgag aagctgaaag agctgactcg cggtaagcgc gttgacgccg
     5701 aaggcatgaa gcagtttatc gatggtctgg cgttgccaga agaagagaaa gcccgcctga
     5761 aagcgatgac gccggctaac tatattggtc gagctatcac catggttgat gagctgaaat
     5821 aaactcgtat cagtgccgga tggcgatgct gtccggcctg tttattaaaa ttatccactt
     5881 tttctttttt cactttacct cccctccccg ctggtttatt taatgtttac ccccataaac
     5941 acataatcgc gttacactat tttaataatt aagacaggga gaaataaaaa tgcgcgtact
     6001 ggttgttgaa gacaatgcgt tgttacgtca ccaccttaaa gttcagattc aggatgctgg
     6061 tcatcaggtc gatgacgcag aagatgccaa agaagccgat tattatctca atgaacattt
     6121 accggatatt gcgattgtcg atctcggatt gcctgacgag gacggtctgt cactgattcg
     6181 ccgctggcgt agcaatgatg tttcactgcc gattctggta ttaaccgccc gtgaaagctg
     6241 gcaggacaaa gtcgaagtat taagtgccgg tgctgatgat tacgtgacta aaccgtttca
     6301 tattgaagag gtgatggcgc gaatgcaggc attaatgcgg cgtaatagcg gcctggcttc
     6361 acaggtcatt tcgctacccc cgtttcaggt tgatctctct cgccgtgaat tatctattaa
     6421 tgacgaagtg atcaaactga ccgcgttcga atacaccatt atggaaacgt tgatacgcaa
     6481 taatggcaaa gtggtgagca aagattcgtt aatgctccaa ctctatccgg atgcggagct
     6541 gcgggaaagc cataccattg atgtactgat gggacgtctg cgcaaaaaaa ttcaggcaca
     6601 atatccgcaa gaagtgatta ctaccgttcg cggccagggc tatttgttcg aattgcgctg
     6661 atgaaaaaat tactgcgtct ttttttcccg ctctcactgc gggtacgttt tctgttggca
     6721 acggctgcgg tagtactggt gctttcgctt gcctacggaa tggtcgcgct gatcggttat
     6781 agcgtcagtt tcgataaaac tacgtttcgg ctgttacgtg gcgagagcaa tctgttctat
     6841 acccttgcga agtgggaaaa caataagttg catgtcgagt tacccgaaaa tatcgacaag
     6901 caaagcccca ccatgacgct aatttatgat gagaacgggc agcttttatg ggcgcaacgt
     6961 gacgtgccct ggctgatgaa gatgatccag cctgactggc tgaaatcgaa tggttttcat
     7021 gaaattgaag cggatgttaa cgataccagc ctcttgctaa gtggagatca ttcgatacag
     7081 caacagttgc aggaagtgcg ggaagatgat gacgacgcgg agatgaccca ctcggtggca
     7141 gtaaacgtct acccggcaac atcgcggatg ccaaaattaa cctttgtggt ggtggatacc
     7201 attccggtgg agctaaaaag ttcctatatg gtctggagct ggtttatcta tgtgctctca
     7261 gccaatctgc tgttagtgat cccgctgctg tgggtcgccg cctggtggag tttacgcccc
     7321 atcgaagccc tggcaaaaga agtccgcgaa ctagaagaac ataaccgcga attgctcaat
     7381 ccagccacaa cgcgagaact gaccagtctg gtacgaaacc tgaaccgatt gttaaaaagt
     7441 gaacgcgaac gttacgacaa ataccgtacg acgctcaccg acctgaccca tagtctgaaa
     7501 acgccactgg cggtgctgca aagtacgctg cgttctctgc gtagtgaaaa gatgagcgtc
     7561 agtgatgctg agccggtaat gctggagcaa atcagccgca tttcacagca aattggctac
     7621 tacctgcatc gtgccagtat gcgcggcggg acattgctca gccgcgagct gcatccggtc
     7681 gccccactgc tggacaatct cacctcagcg ctgaacaaag tgtatcaacg caaaggggtc
     7741 aatatctctc tcgatatttc gccagagatc agctttgtcg gtgagcagaa cgattttgtc
     7801 gaggtgatgg gcaacgtgct ggataatgcc tgtaaatatt gcctcgagtt tgtcgaaatt
     7861 tctgcaaggc aaaccgacga gcatctctat attgtggtcg aggatgatgg ccccggtatt
     7921 ccattaagca agcgagaggt cattttcgac cgtggtcaac gggttgatac tttacgccct
     7981 gggcaaggtg tagggctggc ggtagcccgc gaaatcaccg agcaatatga gggtaaaatc
     8041 gtcgccggag agagcatgct gggcggtgcg cggatggagg tgatttttgg tcgccagcat
     8101 tctgcgccga aagatgaata aatatgtcca tacttcacgc attacgttaa gcatccgtta
     8161 taatcggttg cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac
     8221 tggcccgatt ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt
     8281 aataatttta ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa
     8341 gttgacagtc gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc
     8401 gaaagctacg atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtgaaccac
     8461 tggcatgagc cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt
     8521 gatgatctga tgatctcttt ttctgtaccg ggcggcggcg tcggcccgca tctcgatcag
     8581 tacgacgtgt ttatcattca gggtaccggt cgtcgtcgct ggcgagtggg cgaaaagctg
     8641 caaatgaaac agcactgtcc gcacccggat ctgttacagg tcgatccgtt cgaagccatc
     8701 atcgatgaag agctggagcc tggcgatatt ctttatattc cgccaggatt cccgcatgaa
     8761 ggctacgcgc tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg
     8821 gaattaatta gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac
     8881 agcgatccgg atgttccacc tcgcgctcat cctgcggacg ttctgccgca agagatggat
     8941 aaactgcgtg agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt
     9001 ggcgagttta tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat
     9061 cagccagatg aaatctacga tgcgctgaaa caaggtgaag tgctggtgcg cctgggtggt
     9121 ctgcgcgtat tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg
     9181 caccgtccgg cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc
     9241 gatgcgctgg aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat
     9301 tggttcttcg aagggtaagt ttggatttaa gccggatgcg gcgctgattg cgccttatcg
     9361 tgcctacatg cactgaacgc ctgtaggcac gataagtatg ttgaccagta tcaatataac
     9421 ggtttcatcc cggtaattgg cagtccacat acgcatcggg caatcacgac ttccgttgcg
     9481 ccgttaactc ggcaatacgg acaatcacct gcaccgcttt ttccatacct tccagagtca
     9541 caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg cacggtaatc
     9601 ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt tccggttcaa
     9661 tattgcagtc acgcatcgcc tgctgggcga tatcgataat atgcggatgc tcaaccactt
     9721 tctcgcgcat attgtagtaa ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta
     9781 accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt
     9841 tacggtcgaa atcacggatg atgtagtgca tatcggctcg gtcaacggtg cctttcatgc
     9901 tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa
     9961 cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc
    10021 ccggatgaac attgttaccg acaattttga tattgaccga tgcggcgttg aagttttcaa
    10081 actccagttc acctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat
    10141 caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa
    10201 tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa
    10261 taccggcttt gtcatcggca cctaacaagg ttttaccatc ggtagtaatc agcgtctgac
    10321 ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca
    10381 gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attcacattt ttgccgctgc
    10441 aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt
    10501 tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca
    10561 tctcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa
    10621 cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa aatcgctcaa
    10681 gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa
    10741 gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc
    10801 ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg
    10861 ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg
    10921 gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc
    10981 gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat
    11041 tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg
    11101 ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg
    11161 catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac
    11221 tgttttccaa agctacgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg
    11281 gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg
    11341 gatggtgcaa ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca
    11401 gcaacgcgtc gccattgccc gcgcggtggt taacaagcct cgtctgttgt tgctggatga
    11461 gtcgctctca gcgctggatt acaaactgcg caagcaaatg cagaacgagc tgaaagcgtt
    11521 acagcgtaag cttggcatta cattcgtctt cgtgactcac gaccaggaag aagcactcac
    11581 catgtcagac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg gcacgccgcg
    11641 tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg aaattaatat
    11701 gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca acgttgaagg
    11761 ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct
    11821 gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct
    11881 gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact
    11941 ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgacc ctgactttga
    12001 ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc
    12061 tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg
    12121 tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg
    12181 acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg ctcgatccgc
    12241 tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc
    12301 tggtgctggg ctacccgttt gcctggtttc tggcgaagct gccacacaag gtgcgtccgc
    12361 tgctgctgtt tctgctgatt gttcccttct ggaccaactc attgattcgt atctacgggc
    12421 tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta
    12481 tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca
    12541 ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagcctt
    12601 tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttgca gacctttatc cgcatcatta
    12661 tcccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg ccagcgatgg
    12721 gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca
    12781 tcaaggtaca gttcctcaat attcgtgact ggccatttgg tgcagccacc agcattacgc
    12841 tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt ctgctgaata
    12901 agaaggcaac agaaatagat aattagtgct ttatattcaa aaggttaata gcaaatttgg
    12961 catgcttttt actgtctagt aaacagcatg cctctatcca taaaatcaaa cagttgcaat
    13021 ctaattttgg agaacgtttt ttcttctccc ttttagcaaa tcgctataaa aaacaacaaa
    13081 tggtataaaa atgagcaatc ctaatccatg catgacggac tggagaaagt attcgcaaaa
    13141 gttaaaatca acagtctgtt gattttgaga tataactctc ttttctaata ttgacacctt
    13201 ggctttgctg gttgctaaaa ttagcttgtt gattagcagt atggatagaa cttacactgt
    13261 tatttacttc taaaaatgga atttatcaat gcttcctaca tcgcaattac gaccgtccgg
    13321 gacattctac tccaattccg cagaaacatc agcaggcatc aaaagcgaaa tcacaccaac
    13381 tcagatagaa gaagcgcggg ccattggtcg tttatatatc aaagattgtg atattgagta
    13441 tctgccacag ttaccaaacg aaataacatc agttacaatc gaaaattgca ataacctgac
    13501 cacccttaca ggattaccgg ttaatacaca aaacctgtcc gtcattaact gtgaaaaatt
    13561 acaaatcaca gacataccat cgacagtaaa aaatctacat attgaattaa ctgattcacc
    13621 atttatacac atcatatctg aaggcatcga atgcctgacg gtttgccact gccatatatc
    13681 tggagtgcca gagagtgtcc gtcatcttga gatcagaggt tacgccacag acggcataaa
    13741 aaatgttcca aacgggttat tatctctcag catcaatcgc tacaccccag aaaatcaggc
    13801 cagaattgac agcctgatat caccgtcact acagacgcta tctctgactg gatgtagcaa
    13861 tattatactg ccggagaaac tcccggagag tctgagatct gtaaccattc atgtggagca
    13921 gaaaactaca tggaacatcg tagctgaagg ggttcctgat ggactggatc ttgatttaca
    13981 aaatgtacta ctctctccga atgtaattaa agaaaaaaac atcacttttc agggcaacgc
    14041 actggatgct gccttgcatt ttcgtccggg agacattgtc tatgggctat cctctcccag
    14101 agaaaaactt gtcaacagca tcaaactggt taacgacttt tccaaaaaag atatcgtaat
    14161 tcagaataca ttaacaaacg cagtatggga cgccagaacc ccccgtaaat ataagcaaga
    14221 ctcgcttatc aaaagagcat taaatgaaca tgaaagagga acaaaattta aacagtgctt
    14281 aaaaaatcac aataattata atgtcatcat ggccgacctt tccatataca atcgagacaa
    14341 gttatgggca aaaacaagca aggcaggcct agagtttcaa acattaattc gcaacagaac
    14401 ggttatgttt tgtgcggatg ggcttgtcaa ttcactcaaa ctcatagcta acaagtcaga
    14461 gggctatggt cagagtatta ccgccagcga attacgatgg atttatcgta ataaagacaa
    14521 cagccaaata atgaaaaaca taaaatttta tctgcatggc aaagagatac cagcagaaag
    14581 aatattagat acaccagagt ggaaagacta ccgtccaaaa tactccggtt ccacatataa
    14641 atattcttaa tgatagcaaa aaatatattt tcgatataat caatgttatg attaaagagt
    14701 atttcatcag ggcaggtaaa aacagagtaa atcagcaaaa gaagctgatc ttcagcgata
    14761 ctgacactaa ctgacggttt aagcggtcgt atgaagcagc agctttccga cggactgcca
    14821 tgcggatcgt ttaccttttg ggctattccg cccgtcatca agcggctcac gagtactgag
    14881 tttatcagga tgatattgct gacaatggta attcgttgac cgatgtgtac ttcactatat
    14941 atcggtcaac accgtactgc cgtgcaacat ctcaaagatg tagcatcgtt tttaccagag
    15001 gaggcaggac atagattgta aaaaaactat ttcgatgcat ttaccattga gatattttcc
    15061 ttcataccaa aagttctatc aattatcgca agtggttcaa caggtgcggt cttatctata
    15121 acccaaccac tctctgtaac acttcctcgt gtgatatatt ttgcctttac tttaacaact
    15181 attagataat gctcacgact aaaccgatca gcaacgctaa gatccgatgt gtattcaggc
    15241 aatattcttc caatacgtac ctgctgttgt gccatatatt catctggtgc ttttacattt
    15301 ggatttgaat caggaagccc tccagcagtt ttatattcaa cgatattttc agcttgaata
    15361 ccggttgtgc cgcgacacaa tatgatgtct gaatcggaca gttctatgtg cccctcctta
    15421 attttcgaat aaatttgttt aatactatgt tctggagttg gtactatttc agaagataaa
    15481 gcaggcaacg tgtcttcaag caccggagaa tcatcgtata tatccaacaa atcctcatca
    15541 tacccaatat ctccttctct taactgagaa tacggtttat cta
//
LOCUS       DAZGCS010000111        15331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGCS010000111 DAZGCS010000000
VERSION     DAZGCS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.113"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..293)
                     /locus_tag="ACHK01_003916"
                     /pseudo
     CDS             complement(1..293)
                     /locus_tag="ACHK01_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(592..756)
                     /locus_tag="ACHK01_003917"
                     /pseudo
     CDS             complement(592..756)
                     /locus_tag="ACHK01_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(882..1283)
                     /gene="eptO"
                     /locus_tag="ACHK01_003918"
                     /gene_synonym="gspS"
     CDS             complement(882..1283)
                     /gene="eptO"
                     /locus_tag="ACHK01_003918"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO8098789.1"
                     /translation="MRINTLKKLNCITSLLVLVTVAGCHQVPSTRKQATVPPSEQLEQ
                     TASIAAATRYLKRRCNRSDLPDDQAILNGVNRIANGKGWQSLTQEDIRKHSDEINERL
                     ARDSTPEHIKCSEFNRLLVPFIGELLAGTSR"
     gene            complement(1377..2135)
                     /locus_tag="ACHK01_003919"
     CDS             complement(1377..2135)
                     /locus_tag="ACHK01_003919"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO8098790.1"
                     /translation="MGSFLNVVIHRLPVILERRWRREALMLLAHSEPESEPVFNLMIP
                     RSHCPYCFQPLSIRDNIPLLSYLFLKGKSRCCGERIPIYYPLVEITSSVLFILAASRF
                     HPGLTLVAAWLFIAMLLVLAVIDCRTAMLPDVLTLSLLWLGLLFSLQGGRVPLEEAVV
                     GAVSGYLCLWGLYWLFRFVTGREGLGYGDFKLAAALGAWMGWQVLPSVLLFASVSGLI
                     ITVLLRFVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            complement(2262..2777)
                     /gene="gspM"
                     /locus_tag="ACHK01_003920"
     CDS             complement(2262..2777)
                     /gene="gspM"
                     /locus_tag="ACHK01_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO8098791.1"
                     /translation="MNDLKKRLQYISPRERRLLTGCVALLVVAFVYYVFWLPWQVRED
                     KWRTVIAREKYTVEWMKQQIPSIKKWEASMPEQGEPLGLSAAVTQTSAAYGVSVTRLQ
                     PQGERLTVTLAPVEFNTLMQWLTQLHRRYRIRTVVFDVVAQGNQPGHVTVNRLVLSRD
                     DINRDTSPGHL"
     gene            complement(2764..4041)
                     /gene="gspL"
                     /locus_tag="ACHK01_003921"
     CDS             complement(2764..4041)
                     /gene="gspL"
                     /locus_tag="ACHK01_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8098792.1"
                     /translation="MERGAFPEHSATVWINGEGGTLNKNNKHVEAGRLFLRLPEDDTQ
                     PLLWRLVSPEETGGGSVAVGETDPLLAGLLPRYPAWVLVPSDRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQTGGRCNVAVVEKSVMHHWLTYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVRLAGQWLFRSDRYAGMTVELSWLTQFLSLTAPPVIESYSV
                     PPAQCMGLESTEWRTQPVRDLLQLAAECEGYDGADLRQGIFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAEFWQHESVRVYQELFPGETVVKDPHRQMLRHLQ
                     QSTSNNMPELGGVMHQLQQLLPETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     SAGEKYIIQPGEIRQQPHGVESRLILRMNNERS"
     gene            complement(3975..4952)
                     /gene="gspK"
                     /locus_tag="ACHK01_003922"
     CDS             complement(3975..4952)
                     /gene="gspK"
                     /locus_tag="ACHK01_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8098793.1"
                     /translation="MKSREQGVALLVVLLILSLMVTIAAAIAERNGRTFWRTVAQLDQ
                     LQAKWDGYAAEEMAIQILQRSRQASPRKTHLAQNWAQPELQFETGGGDVRGRIVDAQA
                     CFNLNAINYGAVDLAGVPYAARIFQQLLINLQVDIIQARQVTAALRDWIDRDDEPVKE
                     GAEDEVYMGMEPPYLAASQPMQDVSELRLIRGIDVRLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPNSLLVTKLLLRRPRTGWDSVAAFLDSPQLKGIDTSTAISV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRSIQRQYGLTVREVP"
     gene            complement(4949..5548)
                     /gene="gspJ"
                     /locus_tag="ACHK01_003923"
     CDS             complement(4949..5548)
                     /gene="gspJ"
                     /locus_tag="ACHK01_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8098794.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQSGIRAHELSQD
                     KVRRLAELQRGGSQIERDLMQMIPRHSRGSEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFYVDHPSGIAPDVRVVLEGVHAFRLRFFVNGT
                     WQARWDSTSILPQAVEVTLVMDDFAELTRLFLVSKETAE"
     gene            complement(5545..5910)
                     /gene="gspI"
                     /locus_tag="ACHK01_003924"
     CDS             complement(5545..5910)
                     /gene="gspI"
                     /locus_tag="ACHK01_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8098795.1"
                     /translation="MKRQEGMTLLEVMVALVVFALAGIALIQSTTQQAAGIGPMEEKV
                     LAGWLADNQMVQLQLEKIWPKNGWDEKTISFAGREWYLRWHGRDSDIPEQRILEVEVR
                     RTREETSALVSLRGSVVRE"
     gene            complement(5907..6458)
                     /gene="gspH"
                     /locus_tag="ACHK01_003925"
     CDS             complement(5907..6458)
                     /gene="gspH"
                     /locus_tag="ACHK01_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8098796.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSMQDGLLIGIQVRSDGWTFKVLQRVTAESFPASVGGDVWQGYVWQTWQ
                     PRRAAMEGKLPDDVRLDLQYLRGLQWMPEHNDGTGPDILLLPGGEVTPFSLLFRLVGG
                     EAVTGLQVDENGLMTLFEGEVEL"
     gene            complement(6455..6889)
                     /gene="gspG"
                     /locus_tag="ACHK01_003926"
     CDS             complement(6455..6889)
                     /gene="gspG"
                     /locus_tag="ACHK01_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8098797.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     VSDLVALESALDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSNIDIFSPGPDGIPNTEDDIGNWTPGHTQP"
     gene            complement(6920..8143)
                     /gene="gspF"
                     /locus_tag="ACHK01_003927"
     CDS             complement(6920..8143)
                     /gene="gspF"
                     /locus_tag="ACHK01_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8098798.1"
                     /translation="MALFHYQASDIHGRKRRGVLEADSARHARQLLREQALIPVRLEE
                     KQLHHQHSPRSILRFRPRGVSSAELALLTRQLSTLVAASLPLEEALDALSRQSEKPRQ
                     RDLIAAVRARVLEGHSLATAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALIYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGL
                     TEVCQTWGPWLLLAAATGWIAGRMMLHKPSRRLAFHHLLLRLPIVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMYISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPS
                     MMRHMIASGENSGELDSMLERAADNQDREFSAQMQLALGLFEPMLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNI"
     gene            complement(8145..9647)
                     /gene="gspE"
                     /locus_tag="ACHK01_003928"
     CDS             complement(8145..9647)
                     /gene="gspE"
                     /locus_tag="ACHK01_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8098799.1"
                     /translation="MSSIQNVSESRPLLPFSFSRTQRILLLREQEGNQVFCMEDTPAS
                     ALLEVRRVAEGPLNVTTLSAEAFEKQLVSCYQRDSDEARQMMADIGNEMDFYTVAGEL
                     PDSEDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREILR
                     LRRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVL
                     RLLDKNSVSLDLAALGMSPQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSRLN
                     PQDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDSET
                     AQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCP
                     RCRQSCQASAELAKDMDIPPETTIWQPAGCQHCSFTGYHGRTGLHELLLIDDRIRMAI
                     YQGEGELSITRLAGGRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            complement(9644..11614)
                     /gene="etpD"
                     /locus_tag="ACHK01_003929"
     CDS             complement(9644..11614)
                     /gene="etpD"
                     /locus_tag="ACHK01_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO8098800.1"
                     /translation="MFRKWLNGRLSIFVFTTVFLSAIPGWGAEFSASFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDASPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVVKRLMEIVERVDKEGNRSVATVPLTYASATDVARLVTELTKES
                     DKTAMPAWMTAKLVADERTNSVLVSGEPISQQRIISMIKQLDRKEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSNIENDAKKSSSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEVQDADGLNLGIQWVNKHSGVTQFTSTGLPITTMVQTR
                     QSDISDSDQSNTLSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFSSKSEHAAKRNLMLFIRPTIIRERDGFRHASAEKYLSFNQEQVQSRGKETMALTL
                     NEEQLKLSRDRDDTTFRKVKTAIAAFYARGMQ"
     gene            complement(11615..12490)
                     /gene="gspC"
                     /locus_tag="ACHK01_003930"
     CDS             complement(11615..12490)
                     /gene="gspC"
                     /locus_tag="ACHK01_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8098801.1"
                     /translation="MLFFLSSRRDRNLFIKDIALKMLTPNWVLCVILLIAGYQLVSVI
                     RHFWLTPATSASDLSHVSVSETAVTDEHTEENFVFTLFGTASPPLSEGKVQKTTSSLS
                     DDLLSGGDLDVRGILYSSVTEHSVAIFAHNNRQFSLGIGEKVPGYDATISAIFSDHIV
                     INYQGKNASLPLRYDNPAKRNAQDDNNLIVGPVTTQANFRVKNIFDIMSLSPVTVNNT
                     LSGYRLSPGKASSLFYNAGLHDNDLAVLLNGSELRDTRQAKQIMKQLTELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(12574..15234)
                     /gene="stcE"
                     /locus_tag="ACHK01_003931"
     CDS             complement(12574..15234)
                     /gene="stcE"
                     /locus_tag="ACHK01_003931"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO8098802.1"
                     /translation="MKLKYLSCMILASLAMGAFAATAADNNSAIYFNTTQPVNDLQGG
                     LAAEVKFAQSQILSAHPKEGESQQHLTSLRKSLLLVRLVKADDKTPVQVEARDANDKI
                     LGTLTLSPPSSLPDTVYHLDGVPADGIDFTPQNGTKKIINTVAEVNKLSDASGSSIKS
                     YLANNALVEIQTANGRWIRDMYLPQGAELEGKMVRFVSYAGYNSTVFYGDRKVTLSVG
                     NTLLFKYVNGQWFRSGELENNRIAYAQHTWSAELPAHWIVPGLNLVIKQGNLSGSLND
                     INVGAPGELLLHTIDIGMLTTPRGRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLK
                     EVMLPTGTLLTDADPGEGGWHSGTMRQSIGKELISHGIDNANYGINSTAGSGESSHPY
                     VAAQLTAHTNRGNYSNGVQVHGGSGGGGIVTLDSTLGNEFSHEVGHNFGLGHYVGGFR
                     GSVHRSADQINSTWGWDSDKKRFIPNFYPAQTNRKSCLDEQCQEPFDGHSFGFDAMAG
                     GSPFSDANRFTMYTPNSSAIIQRFFENKAVFDSLSSTGFSKWNADEHKMEPYEHTIDR
                     FEQITASVRDLSENKMAEMMAEYPIVKVHMSDGNWTRDIHAPAASAENKGRILTLDHE
                     AGYNSHLFINGGEKIISKGYKKSFVSDGQTWKESDVVNTREARKPEKFGVPVTTLVGY
                     YDPKGTLSSFIYPAMYGAYGFTYSDDSENLSSNDCQLQVDTREGQLRFKLANNRVNSS
                     VMNKFHINVPTESQPTQAILVCNNKVLDTRTLTPAPEGLTYTVNGRALPAKENEGCIV
                     SVKTAQRYCLPVGQRSGSSLPDWIAGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGN
                     VNPADMKKVKAWNGQYLDFSRPRSMRVVNK"
BASE COUNT         3796 a         4380 c         3447 g         3708 t
ORIGIN      
        1 tattctccgg tcatctgcag cagttttttg gcatcgtgac ttctgagcaa cggaatgcca
       61 gcctcataac ctcttccaga ctaatatccc ggcgccagcg ttccagtcga aaatgacagg
      121 tatcatccag ctctactgct gcaacaatac tgaacgagcg ccgttcaacg gacgatatca
      181 gcccgtacct gcggacacag ataccaccat ttcatatcca cgattttgga cagtcctttc
      241 ataaaactga cttccctttc tgtcagcagc gtcttctgac ctcaccagta ttcactctgc
      301 atcaccgatg ccgtatacat gaacaagaca aattggcgtg atggttatgt gaaagaggag
      361 aaaatgccga tgtttcagtt aggtattgat gtcagtaaaa acaatcttga tctatgcctc
      421 ttacggggga cattaaaggg aagatcaaaa ctcgtaagct aaaaaatacc gctgatgcgg
      481 ctaaaactgt ggcagagtgg cttaaaaagc aatccagtga actgaacgaa gttcatatca
      541 tcatggaagc cactggggta tatcatgaac ctctagctta tggccttcac tttgctggcg
      601 ctcagtggca gttagtccat cattaccgct cccggtagcc cgctcatgct ggcgccccca
      661 gacacgtaga gtttctggcg tacagctaat ctttggggca atgaaacaaa ttgccgcccg
      721 ttgtaagtca tattcgccct gacgcaccag aatcatacgg accgtccgct aacggacttc
      781 cggggaaaaa cgtgtatttt tggtcatcct gtttacctct ttctcaggga gtttagtctc
      841 ctagatttcc gaggcgcggt tcagtacctg tcagtaccct attatctgct agtccctgcc
      901 agtaattcac cgataaatgg gaccaacagc cgattgaact cactgcattt tatgtgctca
      961 ggtgtgctgt ccctagccag acgttcattg atctcatcgc tgtgtttacg tatatcctcc
     1021 tgagttaagg actgccaccc tttcccgtta gcgatccggt taacgccatt cagtatcgcc
     1081 tgatcatctg gcaaatcact gcggttacat ctccttttca gataccgtgt cgcagcggcg
     1141 atcgacgccg tttgttctag ctgttcgctc gggggaacgg tggcctgctt acgagtggaa
     1201 ggtacctgat ggcaaccggc gacagtcacc aggaccagta atgacgtgat acaatttagt
     1261 tttttcaacg tattaatcct catattttta accaggtatt gtttaaccgg tttccggttt
     1321 actatagtcc cgttattctc tgagccattt ttattaccag ccccggaaac cgaacgctac
     1381 atcaacaaaa aatggcatac tccgctcaag gcgagccacg gaccaaaagg cagcggccgg
     1441 gtaaaatccg tggctgtaac aaaacgcagc agtaccgtta tgattaatcc gctgactgag
     1501 gcaaacagaa gcacggaggg taatacctgc caccccatcc atgctcccag tgccgcagct
     1561 agcttaaaat caccgtagcc taacccttct ctaccggtaa cgaaacgaaa aagccagtac
     1621 agcccccaca gacagagata cccagagacc gcccccacta ccgcctcctc caagggcact
     1681 ctccccccct gtaagctgaa taacaggcca agccagagca gggataacgt cagcacatcc
     1741 ggcagcatag ccgtacgaca gtcaataacc gccagaacca acagcatggc aataaaaagc
     1801 caggcagcaa ctaatgtcag cccgggatga aaccggctcg ctgccagaat aaataacact
     1861 gaactcgtta tctcgaccag cggatagtaa ataggaatac gctcaccaca acagcgtgat
     1921 ttccccttca gaaagagata gcttaacagg ggaatgttat ccctgatgct gagcggctga
     1981 aaacagtagg gacaatgaga gcgcggtatc atcaggttaa agaccggctc ggactcaggc
     2041 tcagaatgtg ccaacaacat cagcgcttca cgccgccacc gcctctccag tattaccggc
     2101 agacgatgaa tcacaacgtt aagaaaacta ccgataatgc cccctagccc ccctgtgctg
     2161 atgagccaca acaaatgaaa atcagccgca aattccggca acctgctcac atatcctcct
     2221 gcttatcact cccattttca cgtcaggcat taacctgtaa atcacaagtg ccccggcgaa
     2281 gtatccctgt ttatgtcatc ccgggacaaa accagccgat taaccgttac atgtccgggc
     2341 tggtttccct gcgcgaccac atcaaatacc acggtacgaa tacggtacct gcgatgcagt
     2401 tgcgtaagcc actgcatcag cgtattaaat tcaactggtg ctaaggtaac ggtcagacgc
     2461 tccccctgag gctgcaatcg ggtgactgat acaccatatg cggctgaagt ttgagtcact
     2521 gccgcagata aacctaacgg ctccccctgt tctggcatac tggcctccca tttctttatg
     2581 ctgggtatct gttgtttcat ccactccact gtgtattttt cacgggcaat tacagtccgc
     2641 cacttatcct cccgtacctg ccagggcagc cagaagacat aatagacaaa agcgacaaca
     2701 agtaaggcaa cacatccggt aagcagcctt cgctcacggg gactaatata ctgcaaacgt
     2761 tttttaagat cgttcattgt tcattctcag gatcagacga ctttccaccc cgtgcggctg
     2821 ctgccgtatc tcgccaggct ggatgatata tttttctcca gcgctctgct gaaagtgttc
     2881 cagagcctga aaagaggctg cctgcagatc tattttcaga gtctggctgc tactgtccca
     2941 ggcgagtgcc tgtaagcgta tcgttgaggt ttctggcagc agttgctgaa gctggtgcat
     3001 cacgccccct agttccggca tattgttact cgtcgactgt tgcagatgtc tcagcatctg
     3061 ccggtgggga tccttcacca ccgtttcgcc aggaaacagc tcctgataga cgcgtacgct
     3121 ctcatgctgc caaaattcag cctgctgcca gagccggtaa tgtgccagcc ccgcgttgct
     3181 tccggccaga aacacacagg ccaacagggc ccctgcgaca taccgccatg ggcggaatcg
     3241 cgcataccat gttccctggc gagcaaatat tccctgtcgc agatctgcgc catcatatcc
     3301 ctcacattca gccgccaact gcagcagatc ccgtactggc tgagttcgcc actccgtgct
     3361 ctccaagccc atgcactgtg ctggcggcac ggagtaagac tcaataaccg gcggcgcagt
     3421 aagagacaag aactgtgtca gccacgacaa ctccaccgtc atcccggcgt aacgatcact
     3481 acgaaatagc cactgaccgg caagccttac tgcactccat ccttcagttg ccaacggcag
     3541 catcagtacg tcaggcagca acctcacctc ccggacccca agttgtccac agtacgtcag
     3601 ccagtggtgc atgaccgatt tttcgaccac agccacatta cacctgccac ctgtctgatg
     3661 aagaatggcg aaatgcagct gctcaatatc tgttgccaac tgctcttcca gaagaaaggg
     3721 gagcacctgc aaacgctgac gccacgtacg gtgtggcagc gtgacctgat ggaaggcaat
     3781 tctgtctgac ggaaccagca cccaagcggg ataacgaggc agcagacctg ctaacagcgg
     3841 atctgtttct ccgactgcca cgctgccgcc ccctgtctcc tccggcgaaa ccaacctcca
     3901 caataaaggc tgcgtgtcat cctccggcag tcgcaaaaaa agccggcctg cctcgacatg
     3961 tttgttattc ttgttcaggg tacctccctc accgttaatc catactgtcg ctgaatgctc
     4021 cggaaacgct cctctctcca ctgcattaaa gtctgctggc tgaacaggtg ctccccagag
     4081 cgcacatgta accggaccaa aaaatagttg ctgctaacag ccagtaccga tatggctgta
     4141 ctagtgtcga tgcccttaag ctgtggcgag tcaagaaacg ctgccacgct atcccaaccg
     4201 gtacggggtc gccgcagcag taatttcgtc accaaaagac tgttcggttt cgtcagaaag
     4261 agtgcagcca gcaacggcgc ctgtgaatcc agcagagtat tcacattcac tgacaggtct
     4321 gacgtgggca gtacacaaac atacggtaaa agtttgcggt aaagtctgac atctattccc
     4381 cggatgaggc gcaactcact gacgtcctgc atcggttggc tggccgcaag ataggggggc
     4441 tccatcccca tatacacctc gtcttcagcc ccctccttaa ctggttcgtc atcacgatcg
     4501 atccagtcac gcagagctgc tgtcacttgc ctggcttgta taatatcgac ttgcaggttg
     4561 attaacagct gctgaaaaat tctggcggca taaggcacac cggctagatc cactgctcca
     4621 taattaatgg cattaagatt aaaacaggcc tgtgcatcca ctatccgtcc ccgaacatct
     4681 cctccgccgg tttcaaactg tagttctggc tgcgcccagt tttgtgccag atgcgttttc
     4741 cggggcgatg cctgtctgct cctctgtaat atttgtattg ccatctcctc tgcagcatag
     4801 ccatcccact ttgcctgcag ctgatccagc tgcgccaccg tacgccaaaa tgtcctgccg
     4861 ttacgctccg cgatcgcagc cgcgatcgtc accattaacg acaagatcag caatacaacc
     4921 agaagcgcca cgccctgttc ccgcgacttc attccgcggt ctccttactt acaagaaaca
     4981 gacgggtaag ttcagcaaaa tcatccataa ccagcgtcac ctcaacagcc tgcggcagga
     5041 tgctggtact gtcccagcgc gcctgccaag tgccgttgac aaaaaaacgc agacgaaatg
     5101 cgtgaacccc ctccagcacc accctgacat ccggagcaat accagagggg tgatcgacat
     5161 aaaagtagct caggcgttct aacttttgct gtcgcagccg gtaacctacc cactgtaact
     5221 ccgggcgtgg tagcatcccg gcgggattaa gccaactgtt tcgcgtaaag gaaatccccc
     5281 agtcatcgct tttcagcaga tgaggagcag cgagcagcag gccttcactc ccccggctat
     5341 gtcttgggat catctgcata agatctcgct ctatctggct gccccccctc tgcagctcag
     5401 ccaaacgccg tactttatct tgggacagct cgtgcgcgcg tattccgctc tgtagtacct
     5461 gaaatgcgct gatgctcagc gctgcaaaaa cagccagtgc caacagcatt tccagtagcg
     5521 taaaaccttt cacccgctgc tgactcattc acgaaccaca ctaccacgca aggaaactaa
     5581 ggcggatgtc tcctctctgg tacgccgtac ctctacctct aggatgcgtt gctctggtat
     5641 gtcgctgtcc ctcccgtgcc agcgtaaata ccattctctg ccagcgaagc tgatcgtctt
     5701 ctcgtcccag ccgtttttcg gccagatttt ctcgagctgc agttgcacca tctggttgtc
     5761 agccaaccag ccagccaaca ccttctcttc catcggcccg atccctgcgg cctgctgagt
     5821 cgtggactga attagagcaa ttcctgccag cgcaaacaca accaaggcca ccatgacttc
     5881 tagtaatgtc attccttctt gcctcttcac aactcgacct caccttcaaa tagggtcatc
     5941 aggccgttct catcaacctg cagcccagta acggcctccc ctcctaccag cctaaagaga
     6001 aggctgaacg gcgtcacctc acctccaggc agcaacaaaa tatcgggccc tgtcccgtca
     6061 ttatgctcag gcatccactg cagacctctg agatactgca gatcaagacg cacatcatcc
     6121 ggaagtttgc cctccatggc agcacgccgc ggttgccaag tttgccagac atacccttgc
     6181 caaacatccc cccccactga tgccgggaag ctttcagctg ttacgcgctg caaaacctta
     6241 aatgtccacc cgtcagaccg gacctggatc ccaatcagca agccgtcctg catgcttcta
     6301 tccaaggcat aatccagctg cgcctgcaga cgctcccgct gttgttgcaa atgattctgc
     6361 gctgagtcag gaaatgacat tagtaccaac cctgcagtaa tgcccatcaa caaaaggatc
     6421 agcatcatct ccagtattgt gaatccgcgc tgactcacgg ctgcgtgtga ccgggcgtcc
     6481 agttaccaat atcatcttcc gtgttcggta taccatccgg gccgggagag aagatgtcaa
     6541 tattgctgta ctgaccagga tttaacagct gataatcccc cccccaggga tcttgaggca
     6601 aacgacgtat ataaccatct tgccgatagt tacggggctc aggctgcact gtaggtttac
     6661 tgaccagtgc ccgtagcccc tgctccgttg ttgggtaacg gttgttatct aaccgataca
     6721 tatcgagcgc gctttccagc gcaaccagat cgctaaccac tttctgccga tcggccttat
     6781 ccttatttcc catcagattc gggaccacca gactggccag aactcccaag atcacaatta
     6841 ccaccattat ttccaacaag gtaaaaccac gctgatgctg ttttcgcatg tttttctccg
     6901 ttgactatta aagaagaatt caaatattca tcatattgtt gagttgcagg agcggttgca
     6961 gaattgccag aacaataaac aagacgacgc cagccatccc caccaccaac attggttcaa
     7021 acaatcccag cgccagctgc atctgtgcgc tgaattcacg atcctgatta tcggcggccc
     7081 gttccagcat gctgtcgagt tcgccactat tttcaccgct ggcaatcatg tgccgcatca
     7141 tggacggaaa tagcgaggtc tgctccagcg cctgatgcag gctgacccct tcccgaacca
     7201 attcggctgc ggtggctaac tgatgacggg cccagtcatt actcaacacg tctccactga
     7261 tgtacatagc ctggagtagc ggcaccgcac tggcattaag gatgctcagc gtgcgggcat
     7321 aacgtgcggt attcagccca cgcgatatac gccccacgat cggcaggcgc agcagcagat
     7381 gatgaaaagc cagacgccgg gagggcttgt gtaacatcat acgccccgct atccagcccg
     7441 tggctgcggc tagcagcagc cacgggcccc aagtctggca gacctcagtc agccccatca
     7501 gtacgcgggt agacagagga agggtctgtt tcatatgaat aaactgctca accactttcg
     7561 gcacaacagc agtaagcaga atggcaatca cccctaccgc taccaacgtc aggacacagg
     7621 gatagatcag tgcctggagc agacggtttc gcatgatctg gcgttgctcg gtatagtcag
     7681 ccagacgact aagtaccaca tccaagcgac cggatgtttc acccgccgcc accatcgcac
     7741 aataaagacg ctcaaacgta ccaggaaaca ttcccatcgc cgtggccaag gaatgtccct
     7801 caaggaccct agcgcgcacg gcagcgatta aatcccgctg gcgaggtttc tcactctgcc
     7861 tcgacagcgc gtccagcgcc tcttcaagcg gcagtgatgc ggccaccaag gtggataact
     7921 gccgtgtcaa taatgcaagc tcggcgctgc tcaccccacg aggacgaaat cgaaggatac
     7981 tccgcggtga gtgctggtga tggagctgtt tctcttccag cctaactgga ataagcgcct
     8041 gctcacgcag caactggcgg gcatgtcgag ctgaatccgc ctccagaacg ccacggcgtt
     8101 ttctaccgtg aatatccgat gcctgataat gaaacaacgc catatcattc ctcttcctgc
     8161 aacctgcttt cagtaacgcg aaccacctcc tcccaactgg tctcaccggc tagcaccttt
     8221 tgtcgcccgt cgccacgcag cgtcagatag cgccccccag ccaagcgggt aatactcagc
     8281 tctccttccc cctgatatat tgccatccgg atgcgatcgt caatcagcaa cagttcgtgg
     8341 agcccggtac gcccgtgata gccagtaaaa ctgcagtgct gacacccggc aggctgccaa
     8401 atcgtggttt ccgggggaat atccatgtcc ttcgccagct cagcactggc ctgacaggac
     8461 tgacgacagc gcggacacag cgtgcgaact agtcgttggg ctagaacagc caacaatgag
     8521 gtggaaagca gaaagggggg gatgcccata tcctgcaggc gcgataacgc gcctgcagcg
     8581 ctgttcgtat gtaacgttga cagcaccaga tgcccagtca gtgaggcctg tacagcaatc
     8641 tgggctgttt cactgtcacg gatctcccct acaagcacca catctggatc ctgacgtaaa
     8701 atagcacgca ggctcctggc aaaagtcatg tccaccttcg ggttgacctg ggtctgactg
     8761 ataccgtcca gttcatattc aacaggatcc tctactgtca tgatgttgcg gtcctgtgga
     8821 ttcagccggc ttaacgcagc ataaagcgtc gtgcttttcc ccgacccagt tggcccagtc
     8881 accaaaataa tgccgtgagg acggcggatc agcgcatcaa tgtgtctctg attctgcggc
     8941 gacatgccga gtgcagccag atcgagacta acgctgtttt tgtctagtaa acgcagcacg
     9001 atgcgttcac catggttcga aggcaacgtt gaaacacgca catccacagc ccgtccgcct
     9061 atacgcagca ccatgcgacc atcttgggga acgcgttttt cggcaatatc cagccgagcc
     9121 ataactttaa tgcgtgaaat cagtaacgaa gccagattcc ggcgtaaacg gaggatctcc
     9181 cggagaacgc catcaacgcg aaagcgaacc tgtagatggc gttcgtacgt ttcaatatga
     9241 atatctgagg ctttctcttt aattgcctca gtcagcatgg cgttaatcag gcggataata
     9301 ggcgcatcgt cattcgcatc cagtaagtcc tcactgtcag gcaactcccc cgcgacggta
     9361 taaaaatcca tttcattacc aatatcagcc atcatttgtc gcgcttcatc agaatcacgc
     9421 tgatagcagc tcaccagctg tttttcaaat gcctcagccg acagagttgt gacattcaga
     9481 ggaccttcag ctaccctgcg aacctctagc agggcactgg caggtgtatc ctccatgcaa
     9541 aacacttggt tcccttcctg ttcacgcagc aacaaaattc gctgtgtacg ggaaaaggaa
     9601 aaagggagaa gggggcgtga ttcactgacg ttctgtatac tgctcattgc atcccccgcg
     9661 cataaaacgc agcaatcgcc gttttcactt tccgaaaagt cgtatcgtcc cgatctcggg
     9721 atagcttgag ctgttcctca ttcagcgtca gcgccattgt ttctttacca cgcgactgca
     9781 cctgctcctg attaaacgac aggtattttt cggccgaagc atgacgaaat ccgtcgcgtt
     9841 cacgaataat tgttggccga atgaacaaca tcagattacg cttggctgca tgttcacttt
     9901 ttgagctaaa aagatgtccc agtactggga tatcccccag cagaggtact ttactctcac
     9961 tccctctgat cgacttatcc agtaatccgc cgaccaccac cgtttcgcca ttccctacca
    10021 gcatagcatt ggtcactgtc cgggtattga aggttgcccc caagtcagta gtggtagcta
    10081 ctgctgtatc tgccacaccg gacacctcct gctctatctc cagtaacact gaatcaccct
    10141 cgttgatctg gggttttacc ctgagtttga tccccaccgt tttacgctcg acggtgttaa
    10201 aaatattgtc cccagaggtg gtctgtgaac ccgaaagcac cggaacttcc tgaccaacat
    10261 tgaacgtggc ctccatattg tccagcgtaa caatactggg ggttgccaag atatcatttt
    10321 tgctacttgt ggagagcgcc gtcagcagca tcgcccagtt tccctgataa aaccctgctg
    10381 caatcccgtt aaacatgctc aaggtattgc tctgatcgct gtcagaaata tcgctctgac
    10441 gggtctgaac catcgtggta atgggtaaac cagtactggt gaactgcgtc acaccggagt
    10501 gtttattcac ccactgaatc cccagattca acccgtcagc atcctgtact tcagcaatga
    10561 tggcttccac caggacctga ggacgacgaa tatccaactg cgaaattaca ttttccaagt
    10621 cgcggatgac gtcaggagcc cctgtcagga tcaaggcatt ggtttgttcg tgggatttaa
    10681 tcgtcacccc ttttcgcaga gcttccgttg acgagctctt tttagcgtcg ttttcaatat
    10741 tgctgctgat gcccgtcagg acttccacta aatccttcgc cttcgcatat ttcaggtaaa
    10801 tcaccttcgt attaccctga acatcctcct tacggtccag ttgcttaatc atggagatga
    10861 tacgctgttg ggagattggc tctccgctga cgagaactga gtttgtccgc tcgtcagcaa
    10921 ccagcttcgc tgtcatccag gctggcatgg ctgtcttatc actttcttta gtcagttccg
    10981 tgacaagtct ggctacatcc gttgcagagg cgtaggtaag tgggaccgtg gcaacgctgc
    11041 gattgccctc cttatccacc cgttcaacaa tctccatcag ccgtttcacc acggcagcac
    11101 gaccagtcat cagcagaacg ttagagggtt cataatgcac tacgcttcct gcgccagcat
    11161 tatcattgag ctgacgcagc aagggcgcca agtctctggc tgccacgtta ctgacgggga
    11221 ccacccgggt aacaacctca tcaccagtcc cagggctggc atcactagct accggcaccg
    11281 ccgacgtttt agcatctttt gaacgcacca ctttcagtat accgttgtgc atatcgacca
    11341 cggcaaaacc ataaacatcc agcacgctga gaaagaactg ataatattgt tcctcgttca
    11401 gcatatcgta gctgcgtaca gtgatggtcc cctgcacatc aggattaatt attaccgttt
    11461 tgtgcagatt tttgctgaca gtattaatga actcctgaat atccgtatct ttaaagctgg
    11521 ccgaaaattc agccccccat cccggaatgg cactcagaaa tactgtcgtg aatacaaata
    11581 tcgacaagcg cccgttcaac catttcctga acaatcagtt ctctcctaca gcaataaagg
    11641 catcatacag ttgaccatca cgttcgacgg ttatttttat ttctttaagt tctgtcaatt
    11701 gtttcattat ctgcttcgcc tgtctggtat cacgcagttc tgaaccatta agcaatacgg
    11761 ccagatcgtt atcatgcaac ccagcattat aaaataacga actggcctta ccggggctca
    11821 agcgataacc gcttaatgta ttattcaccg tgacaggtga aaggctcatt atgtcaaaaa
    11881 tattttttac ccgaaaatta gcttgggttg tcacgggtcc gactattaaa ttattatcgt
    11941 cctgtgcatt cctttttgct ggattatcat atcgcagcgg cagtgatgca ttttttccct
    12001 gatagttaat aacaatatga tcgctaaaaa tagcactaat cgtagcatcg taaccaggta
    12061 ctttttcgcc gatgcctaga ctgaactgtc tgttattatg tgcaaatatg gcaacggaat
    12121 gctctgtaac cgaactgtaa agtatacctc tcacatcaag atccccccct gaaagcaggt
    12181 catcagacag ggaagatgtt gttttctgca ctttcccttc cgataacggc ggggatgccg
    12241 tgccgaataa cgtaaacaca aaattttctt ctgtgtgctc atcggttacc gctgtttccg
    12301 acacgctaac atgggataaa tcggacgccg atgtcgccgg agtcagccag aaatggcgta
    12361 taaccgagac cagctgatat ccggcaatga gtaaaatcac gcacaacacc cagtttggcg
    12421 taagcatttt aagtgcgata tcttttatga ataagttccg gtctctccgg gatgatagaa
    12481 agaacaacat aaaacaccgg gcgctattct aggttttgat gaacggaacg aatgtattac
    12541 cgatgcgacc tggtggccgc accgtcttcc ggattattta tttacaaccc tcatcgacct
    12601 cggtctgctg aagtccaggt actgcccgtt ccaagcttta acttttttca tatcagctgg
    12661 attcacatta cccacaaact caccaatcct gttataggac aggttatccc agtcagagag
    12721 cagcactttc gctttggccc cgctgtcgac atagacttcc tggcctgcga tccagtctgg
    12781 cagactggat cctgaccgct ggccaacggg gagacagtaa cgctgggcag tcttcacaga
    12841 aacgatacat ccctcgtttt cttttgccgg aagtgcccga ccatttacag tataagtcag
    12901 accttctggt gctggtgtga gtgttcgggt atccaatact ttgttattac agactaaaat
    12961 cgcctgagtg ggctgacttt cagtcggcac gttgatatgg aacttgttca tcactgaact
    13021 gttcacacga ttattagcca gtttgaatcg tagctgccct tctctagtat ccacctgcag
    13081 ctggcagtca ttactggaca ggttctcact gtcatcagaa taagtgaaac catacgcacc
    13141 gtacattgca ggatagatga agctagacaa agtgcctttg ggatcgtaat aacccaccag
    13201 tgtcgtcacc ggaacgccaa acttctctgg cttacgcgct tcgcgggtat tcaccacgtc
    13261 actttctttc caggtctgcc catcggaaac aaagctcttc ttatatccct tggaaattat
    13321 cttttcacca ccgttgataa acagatggct gttataacca gcttcatgat cgagtgtcag
    13381 aatacggcct ttattctccg cggaagctgc aggggcatga atatctctag tccagttgcc
    13441 gtccgacata tgtactttga caataggata ttctgccatc atctcagcca ttttgttttc
    13501 actgagatcc ctgacagaag cagtaatctg ctcaaacctg tcaatggtgt gttcatacgg
    13561 ttccatttta tgctcatcgg cattccactt gctgaatcca gtggaggaaa ggctatcgaa
    13621 cacagcttta ttttcaaaaa agcgctggat gatagccgat gaatttggcg tatacattgt
    13681 gaaacggttt gcgtcggaga aaggactgcc tcccgccatg gcgtcaaagc caaacgagtg
    13741 accgtcgaac ggctcctggc actgctcgtc aagacaactt ttccggtttg tctgggctgg
    13801 ataaaaatta ggtatgaaac gttttttatc gctatcccag ccccaagtcg agttgatctg
    13861 gtcagcgctg cgatgcacgg accccctgaa gccacccaca taatgaccaa gaccgaagtt
    13921 atgcccgact tcatggctga attcattacc caaggtagag tctagggtaa caattccccc
    13981 tccaccggaa ccaccgtgca cctgtactcc attggagtag ttaccgcggt ttgtatgtgc
    14041 ggtcagttgc gcagccacat aaggatggct gctttctcct gaaccagcag tgctgttaat
    14101 accatagttg gcattatcaa taccatgaga gatcagctcc ttaccgatac tctgtcgcat
    14161 cgtgccgcta tgccatccac cctcaccggg atctgcatct gtcagcaatg taccggtagg
    14221 tagcatgact tccttcagat gaagtggagc ataattgttg acgatcatac gactgaccgg
    14281 gatcgtctgg aaatattccc gatgcgcctc tttgtccttg gcaaaatcaa agcgcccccg
    14341 gggagtggtc agcatcccga tatcgatggt atgcaacaac agctcccctg gtgctccaac
    14401 attgatgtca tttaggctac cactcaggtt accttgttta atcaccaagt ttaacccggg
    14461 cacgatccag tgcgcaggca gttcagcact ccaggtatgc tgagcataag cgattcggtt
    14521 attctccagc tcaccggagc ggaaccactg accatttaca tatttgaaca gcagggtgtt
    14581 acccaccgag agcgtgacct ttcgatctcc ataaaaaacc gttgaattat agccggcata
    14641 cgaaacaaag cgaaccatct taccttcaag ctcggctccc tgcggcaaat acatgtctct
    14701 tatccaacga ccatttgctg tctggatctc caccagtgca ttatttgcca gatagctttt
    14761 aatagaactc ccgctggcat cactgagttt gtttacttca gcaaccgtat taatgatctt
    14821 tttcgttcca ttctgaggag taaaatcgat accatcagca ggaacaccat ctaggtggta
    14881 gacggtatcc ggtagtgagg agggaggcga aagggttaac gtaccgagaa ttttatcatt
    14941 agcatctcgg gcttccacct gaacaggtgt tttatcatca gccttcacca gacggaccaa
    15001 cagcaaactt ttccgcaggc tggtcagatg ttgttgactt tccccttctt taggatgggc
    15061 agataaaatc tggctctgtg caaatttcac ctcagcggcc agtcccccct gcagatcatt
    15121 tacgggctgg gtggtattga aataaatggc tgaattatta tcagcagcag ttgcagcaaa
    15181 tgctcccatc gccagagagg caaggatcat gcatgacaga tactttaatt tcataagtgt
    15241 actccatctt tcattttaga tttcatgcag ccggatataa tagaagtgtt aaattttatt
    15301 ttttcaggag tgattcactc attcaccagt g
//
LOCUS       DAZGCS010000112        15109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGCS010000112 DAZGCS010000000
VERSION     DAZGCS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.114"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            194..1252
                     /gene="citC"
                     /locus_tag="ACHK01_003932"
     CDS             194..1252
                     /gene="citC"
                     /locus_tag="ACHK01_003932"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO8098803.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1267..1563
                     /gene="citD"
                     /locus_tag="ACHK01_003933"
     CDS             1267..1563
                     /gene="citD"
                     /locus_tag="ACHK01_003933"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO8098804.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1560..2468
                     /gene="citE"
                     /locus_tag="ACHK01_003934"
     CDS             1560..2468
                     /gene="citE"
                     /locus_tag="ACHK01_003934"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO8098805.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2479..4011
                     /gene="citF"
                     /locus_tag="ACHK01_003935"
     CDS             2479..4011
                     /gene="citF"
                     /locus_tag="ACHK01_003935"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO8098806.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4015..4566
                     /gene="citX"
                     /locus_tag="ACHK01_003936"
     CDS             4015..4566
                     /gene="citX"
                     /locus_tag="ACHK01_003936"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO8098807.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4541..5419
                     /gene="citG"
                     /locus_tag="ACHK01_003937"
     CDS             4541..5419
                     /gene="citG"
                     /locus_tag="ACHK01_003937"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO8098808.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5470..6933
                     /gene="citT"
                     /locus_tag="ACHK01_003938"
     CDS             5470..6933
                     /gene="citT"
                     /locus_tag="ACHK01_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO8098809.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7047..7853
                     /gene="rna"
                     /locus_tag="ACHK01_003939"
     CDS             7047..7853
                     /gene="rna"
                     /locus_tag="ACHK01_003939"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO8098810.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8083..8493
                     /gene="rnk"
                     /locus_tag="ACHK01_003940"
     CDS             8083..8493
                     /gene="rnk"
                     /locus_tag="ACHK01_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO8098811.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLRIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8631..8720
                     /gene="yldA"
                     /locus_tag="ACHK01_003941"
     CDS             8631..8720
                     /gene="yldA"
                     /locus_tag="ACHK01_003941"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO8098812.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8724..9962)
                     /gene="ybdR"
                     /locus_tag="ACHK01_003942"
                     /pseudo
     CDS             complement(8724..9962)
                     /gene="ybdR"
                     /locus_tag="ACHK01_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10183..10611
                     /gene="uspG"
                     /locus_tag="ACHK01_003943"
     CDS             10183..10611
                     /gene="uspG"
                     /locus_tag="ACHK01_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO8098813.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10732..12297)
                     /gene="ahpF"
                     /locus_tag="ACHK01_003944"
     CDS             complement(10732..12297)
                     /gene="ahpF"
                     /locus_tag="ACHK01_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO8098814.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12426..12989)
                     /gene="ahpC"
                     /locus_tag="ACHK01_003945"
     CDS             complement(12426..12989)
                     /gene="ahpC"
                     /locus_tag="ACHK01_003945"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO8098815.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13361..14107
                     /gene="dsbG"
                     /locus_tag="ACHK01_003946"
     CDS             13361..14107
                     /gene="dsbG"
                     /locus_tag="ACHK01_003946"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO8098816.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14317..>15109
                     /locus_tag="ACHK01_003947"
     CDS             14317..>15109
                     /locus_tag="ACHK01_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8098817.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEI"
BASE COUNT         3676 a         3941 c         3729 g         3763 t
ORIGIN      
        1 ccactaactc tatgtgaaat aaatcaaaat ttcacgccga aatactcctt aggatgtata
       61 gcgaaaggag aaaaagatat acctcgatca cccccctttc tcccaagtga aaataaaagg
      121 ttatcagttt gcaacattga acaacattcg ttgcaaatcg ataacaacat gcaccttcag
      181 gatactattt attatgttcg gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa
      241 aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg acaccacagt
      301 cgaagtattt attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg
      361 aaatattatt aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac tggcgctgac
      421 attagccact gaactgataa acctcgccta tgagcggcac agcacgcatc tgtttattta
      481 taccaaaacc gaatacgagg cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt
      541 acccggcgtg atggtgctga tggaaaacag cgccacgcgg ctgaaacgct atgccgaatc
      601 gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc attgtgatga acgccaatcc
      661 cttcacgaat ggtcaccgtt atctgattca acaagctgcg gcacagtgcg actggttgca
      721 tctgttttta gtcaaagaag attcttcacg cttcccctat gaagaccggt tggatctggt
      781 gttaaaaggc accgccgata ttccacgcct gactgtgcat cgcggctccg aatacatcat
      841 ctcccgcgct acgttccctt gctacttcat taaagaacag agcgtcatta accattgtta
      901 caccgaaatt gatctgaaga ttttccgtca gtacctcgct cccgcgctgg gtgtaactca
      961 ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg
     1021 ctactggctg gaaacgccga ctatctccgc accgcccatc gaactggttg aaattgagcg
     1081 gctgcgttac caggagatgc cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa
     1141 cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt tgcagaacct
     1201 gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag acccccgcat gagaaacagg
     1261 tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg ggatgtgatg
     1321 atacgcatcg ccccactcga tacgcaggat atcgacctgc aaatcaatag cagcgttgag
     1381 aaacagtttg gcgatgcaat tcgcaccacc attctggacg ttctcgcccg ctacaacgtg
     1441 cgcggcgtac agctgaatgt cgatgacaaa ggcgcactgg actgcatttt acgtgcacga
     1501 ctggaagccc tgctggcacg cgccagcggt atcccggctc tgccatggga ggattgccaa
     1561 tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg ttgtttgtgc
     1621 ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta cccggctgat gccctgatgt
     1681 ttgacctcga agactccgtg gcattacgtg aaaaagacac cgcccgccgc atggtttatc
     1741 acgcgctgca acatccgctg tatcgcgata ttgaaaccat tgtgcgtgtc aacgcgctgg
     1801 attccgaatg gggtgttaac gacctggaag ccgtcgttcg cggtggtgcg gacgttgtgc
     1861 gtctgccgaa aaccgatacc gctcaggatg ttctggatat tgaaaaagag atcctgcgta
     1921 tcgaaaaagc ctgtggtcgt gaacccggca gcaccggcct gctggcggcg attgaatctc
     1981 cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg atcggtatcg
     2041 ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga acgctccccg gaaggaactg
     2101 aactgctgtt cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt attcaggcgt
     2161 tcgataccgt ctattccgac gctaacaacg aagccggatt cctgcaagaa gccgcccaca
     2221 tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt gatctgttgc
     2281 acaacctcta cgcaccaacc cagaaagaag tggatcacgc ccgccgcgtc gtagaagccg
     2341 ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag atggtggacg
     2401 gtccggttat cgatcgcgcc cgtctggtgc tctctcgtgc agaactttcc ggcatccgcg
     2461 aagaataagg caatcaaaat gacgcagaaa attgaacaat ctcaacgaca agaacgggta
     2521 gcggcctgga atcgtcgcgc tgaatgcgat cttgccgctt tccagaactc gccaaagcaa
     2581 acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg ccaacctgga agaagcgatt
     2641 cgtcgctctg gtttacagga cggcatgacg gtttccttcc atcacgcttt ccgtggcggt
     2701 gacctgaccg tcaatatggt gatggacgtc atcgcgaaga tgggctttaa aaacctgacc
     2761 ctggcgtcca gctccctgag tgattgccat gcgccgttgg tagaacacat tcgccagggc
     2821 gtggttaccc gcatttatac ctccggcctg cgcggtccac tggcggaaga aatctcccgc
     2881 ggtctgctgg cggaaccggt gcagatccac tctcacggcg gtcgtgtgca tctggtacag
     2941 agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc cgtcctgtga tgaattcggt
     3001 aatgccaacg gctacaccgg taaagcctgc tgcggctccc tcggctatgc aatggttgat
     3061 gccgacaacg caaaacaggt tgtgatgctt accgaagaac tgctgcctta tccgcataat
     3121 ccggcaagca ttgagcaaga tcaggttgat ttaatcgtca aagttgatcg cgttggcgat
     3181 gctgcaaaaa tcggcgctgg tgctacccgt atgaccacta acccgcgcga actgcttatt
     3241 gcccgtagcg ctgcggatgt gattgtcaac tcaggctact tcaaagaagg tttctccatg
     3301 caaaccggca ccggcggcgc atcgctggcg gtaacccgtt tcctggaaga caaaatgcgt
     3361 agccgcgata ttcgcgccga cttcgccctc ggtggtatta ccgcgacgat ggttgatctg
     3421 cacgaaaaag gtctgatccg caaactgctg gatgtacaga gctttgacag ccacgctgcg
     3481 caatcgctgg cccgtaaccc caatcacatc gaaatcagcg ccaaccagta cgctaactgg
     3541 ggttcgaaag gtgcatcggt tgatcgtctc gacgtggtgg tactgagcgc gctggaaatt
     3601 gacacccagt tcaacgttaa cgtgctgacc ggctctgacg gcgtactgcg tggtgcttcc
     3661 ggtggtcact gcgataccgc gattgcctct gcgctttcca tcatcgtcgc gccgctggta
     3721 cgcggtcgta ttccgactct ggtggataac gtgctgacct gcatcacccc gggctccagt
     3781 gtcgatattc tggtcacaga ccacggtatc gcagttaacc cggcacgtcc ggagctggca
     3841 gaacgtctgc aggaagcggg cattaaagtg gtttccattg agtggctgcg tgaacgtgcg
     3901 cgtctgctga ccggtgaacc acagccgatt gaattcaccg accgcgtcgt tgccgttgtg
     3961 cgttaccgcg atggctcggt gatcgatgtt gtacatcagg tgaaggaata agccatgcac
     4021 ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc ccgagctgct cgtcagccgg
     4081 gatgaaaggc aagcacggca acacgtctgg ctcaagcgcc atcctgttcc actggtctcc
     4141 tttaccgtgg ttgcgcctgg gccgattaaa gacagtgagg tcacacgccg aatttttaat
     4201 catggcgtga cagccttgcg tgccttagcc gcaaaacagg gctggcaaat tcaggagcag
     4261 gctgcactgg tttccgccag cgggccggag ggtatgttga gcattgccgc cccggctcgc
     4321 gacctcaagc tcgccaccat tgagcttgaa catagtcatc ctctcgggcg gttatgggat
     4381 atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc gcgactattc actgccgcct
     4441 cgcagctgcc tgttgtgcga gcaaagcgca gccgtctgcg cgcgtggaaa aacccatcaa
     4501 ctgaccgatt tactcaaccg catggaggca ctgctgaacg atgtcgatgc ctgcaacgtc
     4561 aactaaaacc acaaagcttg cgacgtcatt aatcgatgag tacgccctgc tgggctggcg
     4621 cgccatgctg actgaagtca atctgtcacc gaaaccaggc ctcgtggatc ggattaactg
     4681 cggtgcgcat aaagatatgg cgctggaaga tttccaccgc agcgcgctgg cgattcaggg
     4741 ctggctaccc cgtttcattg aatttggtgc ctgtagtgcg gaaatggcac cagaagcggt
     4801 actccacgga ttacgcccaa ttggtatggc ttgcgaaggt gatatgttcc gcgccactgc
     4861 gggcgtaaac acgcataaag gcagcatttt ttctttaggg ctgctatgtg cggcaattgg
     4921 ccgtttgctt cagctcaacc agccggtaac gccaacaacc gtttgttcta cggcggcaag
     4981 tttctgccgt ggtctgaccg atcgcgaact gcgtaccaat aattcacaac tgacggcagg
     5041 tcaacggttg taccagcagc ttggccttac cggcgcacgc ggtgaagccg aagcgggtta
     5101 tccactggtg atcaaccacg ccttgccgca ttacctcact ctgctggatc aggggttaga
     5161 tcctgaactg gcattgctcg ataccttgct cctactgatg gcgatcaacg gcgataccaa
     5221 cgttgcttcg cgcggtggcg aggggggcct gcgctggcta cagcgcgagg cgcaaacatt
     5281 attgcaaaaa gggggcattc gaacccccgc cgatctcgat tatctccggc agttcgacag
     5341 ggagtgtatc gaacgaaatc tcagtccagg cggcagtgcc gacctgctga tccttacctg
     5401 gtttttagca cagatttaat tatttaagca cttgataaat ttgaaaatat taattttcgg
     5461 agaacccgta tgtctttagc aaaagataat atatggaaac tattggcccc actggtggtg
     5521 atgggtgtca tgtttcttat ccctgtcccc gacggtatgc caccacaggc atggcattac
     5581 ttcgctgtgt ttgtggcaat gattgtcggc atgatcctcg agccaattcc ggcaacagcg
     5641 atcagtttta ttgcggttac tatttgcgtt attggcagta attacctgct ctttgatgcc
     5701 aaagaattag ctgacccagc gtttaatgcg caaaaacagg cgctgaaatg gggtctggct
     5761 ggtttttcca gcaccacggt atggctggta tttggcgcat ttatttttgc attagggtat
     5821 gaagtttccg ggttaggtcg tcgcattgcc cttttcctgg tgaaattcat gggcaaacgc
     5881 acgctgacgt tgggttatgc gattgtcatt atcgacattc tgctggcacc gtttacaccg
     5941 tccaacaccg cgcgtaccgg gggtacggtt tttccggtca ttaaaaacct gccgccgttg
     6001 tttaaatcat tccccaacga tccgtccgcg cgtcgtattg gcggctattt gatgtggatg
     6061 atggtcatta gtaccagtct gagttcgtcc atgtttgtca ccggtgcggc accaaacgtg
     6121 ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt gcagtggttc
     6181 ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct ttcctacgtg
     6241 ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg tgatgaactg
     6301 aaaaccatgg gtgcgctgac acgcagagag tggacactga ttggccttgt attgctcagc
     6361 ttaggtttgt gggtttttgg cagtgaagtc attaatgcta ctgcagttgg tctgctggca
     6421 gtttcgctaa tgctggccct gcacgttgtg ccttggaaag atattacccg ctataacagc
     6481 gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg cctgactcgt
     6541 tctggcttta ttgactggtt cgccggtacc atgagtacgc acctggaagg attctcacca
     6601 aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct gtttgccagc
     6661 ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa aggtattccg
     6721 ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat catgggctgt
     6781 ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta tgtgaaatca
     6841 aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat gttgctgttg
     6901 gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct gaataaatca
     6961 tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag atagtattca
     7021 gtgtatgtat gagttccaca cccattatga aagcattctg gcgtaacgcc gcgttgctcg
     7081 cggtttctct gcttcccttc tcttctgcca acgccttagc gttgcaggca aaacagtatg
     7141 gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc cagagtcaac
     7201 acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg accaacaaag
     7261 ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt gctgcccgtg
     7321 gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc ccgaatctac
     7381 cagaagcgca cgccagccgg atgtgttcat cgccggaaac cggattatca ctggaaacgg
     7441 ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg gaacgctacg
     7501 aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc ggtacgatgg
     7561 tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg gataattacg
     7621 gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg ggaaaagaga
     7681 acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact gaaattcaga
     7741 tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc ttgccacaac
     7801 cacacccagg caactgtggc aaaacctttg tgattgataa agtgggttat taatcatctg
     7861 atttacaaaa cacgctaatt cgtcttccat aaaccgtgac gattaagaat tatacataat
     7921 gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt gagcaacagt
     7981 cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc ttttgggtat
     8041 gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag accaactatc
     8101 atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc cgcctatgct
     8161 ggtttgcgaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat gtgttcgcca
     8221 gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg caatcttagc
     8281 gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga tagcaatact
     8341 cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt tggcgattcc
     8401 attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga actcgaatac
     8461 cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg ataactcgct
     8521 ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt cctggttctc
     8581 actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc atggcagaag
     8641 cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg gctgtccttt
     8701 acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt caccagacct
     8761 gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat caccttacgg
     8821 cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctctta aaacggcata
     8881 tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg taataattct
     8941 cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt atcaaaggcg
     9001 tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct aacaatgccg
     9061 ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc ctccagtttc
     9121 aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac ggcgtctatt
     9181 actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc cggatcgcta
     9241 tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg caagcggtag
     9301 ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca ggcgattgtc
     9361 aacaatccca ctggaccggc accatagact gcaacgcttg aaccttgttg gatctgcgca
     9421 ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc tttatcatct
     9481 gaaagcaaag gcggtacttt aaacggcccc acattccctt tagggacgcg gacatattcc
     9541 gcctgtccac caggaacgcc accatacagg tgactataac caaacaatgc cgcgggagct
     9601 ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc gcaggcggca
     9661 tattgctgca ttcgacagaa aaaacagtcg ccacaagcaa tgacaaacgg aattaccacc
     9721 cggtcgcctt tttgcaaatt ttttacgtcc tttccggttt caacgacttc ccccataaat
     9781 tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata aagatggagg
     9841 tcagagccac agatcgccgt tgccgtaata cgcagaataa tatcatctgc ctgttcaatg
     9901 cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt caatgctttc
     9961 atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct atcacagcgg
    10021 gaaaacgggc ggtaccgcag gttttgggag aattcttggt gttcagtgag aatatattaa
    10081 tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa atattataat
    10141 catggcgaat gatttgttca tgattaacag ggagaaaggg ttatgtataa gacaatcatt
    10201 atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca cgctgaattc
    10261 ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc agccagcctg
    10321 agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca acatgaagca
    10381 gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg cattaaacaa
    10441 catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga actgggggct
    10501 gatgttgtag ttattggttc tcgcaaccca tcgatttcga cccatctgtt aggttctaac
    10561 gcctcgagcg tgatccgcca tgccaatctg ccggtgctgg ttgtgcgtta attgccagaa
    10621 taaatatccc gccctgcccg gagccatccc ggcagggctt tttaatgcca taaaaaagcc
    10681 gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt cttatgcagt
    10741 tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt cgccagtggc
    10801 gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaata cgcctttgac
    10861 gttggtttcg catttcgcat caatgataat ctcacccatg cggttacgtt cgactgcgcc
    10921 ttccagccag ttggtgttcg gcagcagacc aatctggacg aaaataccgg ccagttcgat
    10981 gttgtgaata tcgccgctga cacgatctcg atattccaga ccaacgactt tgctgccgtc
    11041 gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca ggctgcgcag
    11101 tttgtcctgc agcacctggt cggctttcat ttctggcgca aattccagca gagttacgtg
    11161 ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc cgccgataac
    11221 cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt
    11281 ggtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac cggtcgccac
    11341 gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt gtagaccacc
    11401 ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat caacgtcgta
    11461 ttcatcaacg tgaactttca gtgcgcctgc cagcttctgc ccttcagtct tcggtacaga
    11521 aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt cgcccatcag
    11581 accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg ccgggccgga
    11641 accgacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg cacgtttttc
    11701 cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct gaccaaactc
    11761 tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga tttcgttctg
    11821 gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcagtacgc tcatcaggtt
    11881 cagcgcctgc accacgtccg ggcagttgtg gcaagagagc gagtaatagg tttcgaattc
    11941 aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt tcgacggatg
    12001 accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca gcggggagcc
    12061 tgcaaaacgt ggcccctggt tggaacctgg gttggtgatc aggaaagacg gcttacgcac
    12121 cggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga tttcagccaa
    12181 cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta actcaacagg
    12241 cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt cgagcatgtt
    12301 tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct ggatgcagct
    12361 tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgattaatt acgcgattgc
    12421 ggaacttaga ttttaccaac caggtccaga gacggagcca gagttgcttc accttctttc
    12481 catttagccg ggcaaacttc acctgggtga gaagctacgt actgtgctgc tttgatttta
    12541 cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg taacttcgat tgcctggatg
    12601 ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc ttcacgcatg
    12661 ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata tttgatttta
    12721 gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc ggtagatact
    12781 gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac gtcacccagt
    12841 tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct ccagcggcct
    12901 tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg gtttttaaaa
    12961 ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc gatgagatgt
    13021 aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc gataagtttt
    13081 accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc gataaaaagt
    13141 aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta tcttcagcgc
    13201 ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat tacaacaccc
    13261 tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga taggttatgc
    13321 cttttactcg acttttgcac tgactgaaaa ggacaaatta atgttaaaaa agatactttt
    13381 actggctctg cttcctgcaa tcgccttcgc agaggaactt ccttccccag taaaagcgat
    13441 tgaaaaacag ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa tgaaaggtta
    13501 tctcggaaag tatcaggata tgggcgtcac catctacctg actcccgatg gtaagcacgc
    13561 tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac ttatcgaaaa
    13621 agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat cccactggct
    13681 cctcgacggt aaaaaagatg cgccggtcat tgtctacgtg ttcgccgatc cgttctgccc
    13741 atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca aagtgcaatt
    13801 aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag cggcaattct
    13861 tgcctccaaa gatcccgcaa aaacctggca agaatatgaa tcctctggtg gcaagcttaa
    13921 gctaaacgtg cctgcaaacg taagtacaga acaaatgaaa gtgttaagcg ccaacgagaa
    13981 actcatggac gatctggggg caaatgtcac accagcgatc tattacatga gtaaggaaaa
    14041 tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca ttatggggaa
    14101 taaataaaag ataattcatg gatggttgaa atacatccct gcttttcaac catcctaaaa
    14161 aagcatgaat tgctcgctct gccctaagtg gctatgctat tttagaaaaa cttaatcctg
    14221 agttaagcaa aaaaaatata atcaataaaa atatatacgc aaaaaacatt gattaagtga
    14281 atataccatg gaagaaaaat ataaccggag tagtgtatgg ccaatctcta cgacttgaaa
    14341 aagttcgact taaaccttct tgtcatattt gagtgtattt accagcattt aagcattagc
    14401 aaagctgctg aatcactata tatcacccca tcagccgtga gccagtcgtt acaacgctta
    14461 cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc gccgacgaca
    14521 acagggttga atctgcatca ccatcttgag aaaaacctca aaggccttga acaaacaatt
    14581 aatatagtca ataagtccga actaaagaaa aactttatta tttacggccc tcagctcatt
    14641 tcttgctcta ataatagtat gctgatccgc tgcctacggc aagatgcctc tgtcgagatt
    14701 gagtgccatg atatcctcat gtcagcagag aatgctgaag aactgttggt tcaccgcaaa
    14761 gccgatctgg tgatcaccca aatgccagtg atcagccgtt ccgtcatttg catgccattg
    14821 catacaattc gcaatactct aatttgtagt aataagcatc cgcgaattac tgacaatagc
    14881 acttatgaac aaattatggc agaagagttc acccaattaa tatccaaatc agcaggagtt
    14941 gatgatattc aaatggaaat agacgagaag tttatgaatc gcaaaatttc atttcgtggt
    15001 tcctctttgc tgacaatcat taatagcatt gcagttacag atttactggg aatagtcccc
    15061 tatgaacttt ataattccta ccgtgacttt cttaatttaa aagagataa
//
LOCUS       DAZGCS010000113        15079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGCS010000113 DAZGCS010000000
VERSION     DAZGCS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.115"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(249..1301)
                     /gene="fbaB"
                     /locus_tag="ACHK01_003948"
     CDS             complement(249..1301)
                     /gene="fbaB"
                     /locus_tag="ACHK01_003948"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO8098818.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1558..2835
                     /gene="yegT"
                     /locus_tag="ACHK01_003949"
     CDS             1558..2835
                     /gene="yegT"
                     /locus_tag="ACHK01_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO8098819.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2832..3836
                     /gene="yegU"
                     /locus_tag="ACHK01_003950"
     CDS             2832..3836
                     /gene="yegU"
                     /locus_tag="ACHK01_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO8098820.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3833..4798
                     /gene="yegV"
                     /locus_tag="ACHK01_003951"
     CDS             3833..4798
                     /gene="yegV"
                     /locus_tag="ACHK01_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8098821.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4772..5518)
                     /gene="yegW"
                     /locus_tag="ACHK01_003952"
     CDS             complement(4772..5518)
                     /gene="yegW"
                     /locus_tag="ACHK01_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8098822.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYLQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5570..6388)
                     /gene="yegX"
                     /locus_tag="ACHK01_003953"
     CDS             complement(5570..6388)
                     /gene="yegX"
                     /locus_tag="ACHK01_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO8098823.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGTVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQYSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6453..7253)
                     /gene="thiD"
                     /locus_tag="ACHK01_003954"
     CDS             complement(6453..7253)
                     /gene="thiD"
                     /locus_tag="ACHK01_003954"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO8098824.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7250..8038)
                     /gene="thiM"
                     /locus_tag="ACHK01_003955"
     CDS             complement(7250..8038)
                     /gene="thiM"
                     /locus_tag="ACHK01_003955"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO8098825.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8073..8169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8261..8533)
                     /gene="rcnR"
                     /locus_tag="ACHK01_003956"
     CDS             complement(8261..8533)
                     /gene="rcnR"
                     /locus_tag="ACHK01_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO8098826.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8654..9478
                     /gene="rcnA"
                     /locus_tag="ACHK01_003957"
     CDS             8654..9478
                     /gene="rcnA"
                     /locus_tag="ACHK01_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO8098827.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9697..10035
                     /gene="rcnB"
                     /locus_tag="ACHK01_003958"
     CDS             9697..10035
                     /gene="rcnB"
                     /locus_tag="ACHK01_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO8098828.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10117..11151)
                     /gene="yehA"
                     /locus_tag="ACHK01_003959"
     CDS             complement(10117..11151)
                     /gene="yehA"
                     /locus_tag="ACHK01_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO8098829.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11165..13645)
                     /gene="yehB"
                     /locus_tag="ACHK01_003960"
     CDS             complement(11165..13645)
                     /gene="yehB"
                     /locus_tag="ACHK01_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8098830.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGRSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13661..14335)
                     /gene="yehC"
                     /locus_tag="ACHK01_003961"
     CDS             complement(13661..14335)
                     /gene="yehC"
                     /locus_tag="ACHK01_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8098831.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(14416..14958)
                     /gene="yehD"
                     /locus_tag="ACHK01_003962"
     CDS             complement(14416..14958)
                     /gene="yehD"
                     /locus_tag="ACHK01_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8098832.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         3739 a         3778 c         3512 g         4050 t
ORIGIN      
        1 tcgagataac gatcacaaaa acgacaatgt gaaaattgtt cgaggagtaa aaggcaaaaa
       61 aaaacggcct cccgataggg aagccgtagc aaagtgcgcg tgtttttatg ccggatgcgg
      121 tgtaaacata cgtggcagtg ctatgtaggc atgataagac gcttccgcgt cgcatcaggc
      181 ataggttgcc ggatgcagtg taaacgcctt atccgtccta cggggtggtg ctgttcgtat
      241 gaggcgtatc aggcaatagt aattttgcta tcgagataaa cgtcctgcac ggcattaatc
      301 agtttcacgc cgtcagccat cgatttcttg aacgccttac gtccaagaat cagccccatt
      361 ccgccagcgc gtttgttgat aaccgcagta cgcactgcat cactgaggtc agtttcaccg
      421 cctgcagcac cgccggagtt aatcagccct gcgcgcccca tatagcagtt agctaactga
      481 taacgcacca gatcaatcgg gttttcgctg gttaacttgc tgtacacgcg atcgtcggta
      541 taaccgtaat taattgcttt atagccgccg ttattttccg ccattttttg tttgacgata
      601 tctgcaccga tggttgccgc cagatggttt gcctgaccgg tcaggtcggc ggaaacatgg
      661 taatcaacgc catctttctt aaaggcggag ttacgcaaat aggcccacag cactgtcacc
      721 atacccagct cgtgcgcacg ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac
      781 tcttccgagc caaaatagat agtcgcacca accgcaaccg cgcccatgtt gaacgcctgc
      841 tccacgctgg catacagcgt ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga
      901 ttaagtttga cgaggaatgg aatgcgatgc gcataacgcc gcgataccga cgccagcacg
      961 ccgtaagttg acgccacaca gttacagccc gcttcgatcg ccagttcaac aatgtttttc
     1021 gggtcaaagt agagcgggtt agcagcaaat gaagctccgg cagagtgctc aacgccctgg
     1081 tcaaccggca gaatagaaag atatcctgtg ccagccagac gcccggtgtt gtacaacgtc
     1141 tgcatattac gtaacaccgc tggcgggcga ttattgtcaa tcattacgcg gtctacgtag
     1201 tcatgtccgg ggagataaag ctggtcagaa ggaattgtca tacaacggtg ctgtaaaagg
     1261 ttgtcggcgt ctttgccaag caactgcgca atatctgtca ttactatgct cccgtaaatt
     1321 ccgattggat atcggctatg gattgtcctg gcccgccttt tgcgggcaat cataatcctg
     1381 gtcgttacgg gcgagttttt ccatcttttg gatgtttttt cagcgttttc tgttggctcg
     1441 attcatcaga aaaaatgtta gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat
     1501 tattaagtgg tattatcatc gcgtacctta cattacctgt catgaaggaa ttaaaagatg
     1561 aaaacaacag caaagctgtc gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg
     1621 tttgtgccat tgtggttgtg gttaagtaaa agcggtttta gtgccggaga aattggctgg
     1681 tcgtatgcct gtaccgccat tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact
     1741 gaccgctttt tctcggcgca aaaagtgctg gcggtattga tgttcgcagg cgcgctgctg
     1801 atgtatttcg ctgcgcaaca gaccactttt gccgggttct ttccgttact gctggcttac
     1861 tctctaacct atatgccgac cattgcgctg actaacagca tcgcttttgc caacgtgccg
     1921 gatgtggagc gtgatttccc gcgcattcgt gtgatgggca ctatcggctg gattgcctcc
     1981 ggtctggcat gtggtttctt gccgcaaata ctggggtatg ccgatatctc accgactaac
     2041 atcccgctgc tgattaccgc cggaagttct gctctgctcg gtgtgtttgc gtttttcctg
     2101 cccgacacgc caccaaaaag caccggcaaa atggatatta aagtcatgct cggcctggat
     2161 gcgctgatcc tgctgcgcga taaaaacttc ctcgtctttt tcttctgttc attcctgttt
     2221 gcgatgccac tggcgttcta ttacatcttt gccaacggtt atctgaccga agtcggcatg
     2281 aaaaatgcca ccggctggat gacgctcggc cagttctctg aaatcttctt tatgctggca
     2341 ttgccgtttt tcactaaacg ctttggtatc aaaaaggtat tattgcttgg tctggtcacc
     2401 gctgcgatcc gctatggctt ctttatttac ggtagtgcgg atgaatattt cacctacgcg
     2461 ttactgttcc tcggtatttt gcttcacggc gtaagttacg atttttacta cgttaccgct
     2521 tacatctatg tcgataaaaa agcccccgtg catatgcgta ccgccgcgca ggggctgatc
     2581 acgctctgct gccagggctt cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg
     2641 gaaaagatgt tcgcttatcc ggaaccggta aacgggctga ctttcaactg gtccgggatg
     2701 tggactttcg gcgcggtgat gattgccatt atcgccgtgc tgttcatgat ttttttccgc
     2761 gaatccgaca acgaaattac ggctatcaag gtcgatgatc gcgatattgc gttgacacaa
     2821 ggggaagtta aatgaaaaca gaacgtattc tcggtgctct ttatgggcag gcgttagggg
     2881 atgcgatggg gatgccctcc gagctttggc cacgcagccg cgttaaagca cactttggct
     2941 ggattgaccg ttttctgcct ggaccaaagg agaataacgc ggcctgttat tttaaccgcg
     3001 ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg
     3061 aaggcaagat cgatccggat ctgattgggc gtaatattct cgactgggcg ctgcgtttcg
     3121 acgcctttaa caaaaacgta ctaggtccga cctcgaagat tgcgcttaac gccattcgcg
     3181 acggtaaacc cgttgctgaa ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg
     3241 tctcgccatt aggttgtttg cttccggcgc gtgatgttga ttcctttatt gatgatgtgg
     3301 cgctggcgtc gagcccgacc cataaatccg atctggcagt tgcaggtgcg gtagtcatcg
     3361 catgggcgat ttctcgtgcc attgacggag aaagctggtc agcgattgtt gattccctgc
     3421 cttcaattgc gcgacatgca cagcaaaaac gcatcactac cttcagcgcc tcactggcag
     3481 cacgtctgga gattgcgctg aaaattgtgc gcaatgccga cggcaccgaa tccgccagcg
     3541 aacagcttta ccaggtcgtt ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca
     3601 ttgcgctggt tgaactggca caaaccgacc cgaatcgctg cgccgtcctg tgcgctaacc
     3661 ttggcggcga cacagacacc atcggcgcta tggcgacggc gatttgcggc gcgttgcatg
     3721 gcgttaacgc tatcgatcct gcattaaagg cggaactgga tgcggtaaat cagcttgatt
     3781 tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca acgggaggcg gtatgagcgg
     3841 cgctcgatta cacacgctgc tgcctgaatt aacctcgcgt cagccggtga tggtcgtcgg
     3901 cgcggcggtc attgatgtga tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat
     3961 cgaactgaag cagcagagcg ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt
     4021 aaagcgcctc ggcatcgaag cgggtaatgc cttgccgctc ggtcagggcg tgtgggcgga
     4081 gattattcgc aaccggatgg cgaaagaggg gttaatcagc ctgatcgata acgccgaagg
     4141 tgataacggc tggtgtctgg cgctggttga gccggatggc gaacgcactt ttatgtcatt
     4201 cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg
     4261 cagcctgctc tatttttccg gttatcaact ggcctcgccc tgcggcgaat tgttagtgga
     4321 atggctggaa gagctgcaag acgtgacgcc gtttatcgat tttggcccac gtattggcga
     4381 tatcccggat gcattactgg cgcggatcat ggcctgtcga cctttagtgt cgctcaatcg
     4441 tcaagaggct gagattgccg ccgaacgttt tgctttatcc gcagagataa caacacttgg
     4501 cgagcaatgg caggagaaat ttgccgcgcc gttgatcgtt cgtctcgata aagaaggcgc
     4561 atggtatttc agcaacgacg cttctggctg cattccggca tttccaacgc aagttgtaga
     4621 caccattggg gcgggcgaca gtcatgcagg tggcgtactt gccgggctgg cctctggtct
     4681 gccactggcg gatgccgtat tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg
     4741 gggcggtgat tgtgcgccaa cgcgcgagga actactcctc gcacacaaaa acgtatagat
     4801 cgctgcgaca gtggctaatg ctgtactcaa taggccgttg ctgttggtca agcgccactt
     4861 gcttgatcac cagcaccggt attttgctat ctaactgaat atgtgactga aactcggcat
     4921 ccggcatccg ggcgctaacg cgggaacgcg tacgttgtag gtaaatgtgc tggctgcgga
     4981 aatagtcata aagtgaaatc ccgatggcat caacatcatg aattaaatgc gccggaaccc
     5041 acgattcctc aatcgatacc gcttcttcat cgacatagcg aatacgctta agcaaaaaga
     5101 catcacttcc tgcttcgacc gccagctgct gcgcgacttc ttcgggacat ttcacgacac
     5161 gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac
     5221 ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg
     5281 aacgcgtcac cacaccctct tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgcg
     5341 aaacgccggt taactgactt aaatcacgct caccgggcaa aatattgcca tgctccagca
     5401 ccccgctgcg cacggcattt tttaccgttt cggcaaactt aatataaagc ggcgtgttat
     5461 ccgccgctaa aatacgctcg ttcaactgag cgataagctg ggtatgcgct tgttccattt
     5521 gtctttttcc tgccggggag tctggtggga gtatatcact gcttcttatt taaggcgttt
     5581 ctttaatccc atcaacgaat ccctgcaact cctccaccgt gccattaaac acattaaaat
     5641 ccaccggacc attgatgcca tctacctgtc cacggtcgga atactgccag aagcgccagg
     5701 ccattccgtc attgtccgga cgacgttgat aatagtgagc cacccaccac ggatattcat
     5761 taaaatagcc cgccagattg gtgtgataaa aaacggttcc tgagtaaata atcggctttt
     5821 ttcccgtact tttttcgacc atttttagcc actgacttac tcgtttgcgt aattcttttg
     5881 ccgataattt tccccgttct tctacgtcca gtacagcagg gaaatcgcct tgtgagaaat
     5941 ccaccgtttg cagaaataat cgtgcctgaa ccggcgctgc taccgacggg gagaaataat
     6001 gatacgcccc gcgcagcagg ccattttcgc ggcttagttg ccagttacgc gaaaaatacg
     6061 ggtccaccag cttttcgcct tccgtcgcct taataaaagc aaactgtaag cggataccgt
     6121 tgtcgcgcat ttttgccacc cgctgccagt cgatccgctc ctgccagcga gaaacatcta
     6181 ttccgtgaat ggtgtaactg gcaggaatgc gaatagcgaa agatttaacc gggcgataac
     6241 cgtagaaatg gatgtagtct gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct
     6301 gacgcggata aattgcaacg atggaaatta gcccaagagc gcataataga gaggtgagtt
     6361 tttttcgact ggtaattctt agctgcattg gtttcatccc tgaatgtcag tgccaaaggc
     6421 tgacaataac caaagcagct atagtacggt gctcaccacc aggcgtggaa gtggtgaacc
     6481 ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg ctaatgctga tgaaagccag
     6541 ctttttgcct cctgtacggt gtcagcccag tttgtatggc gcgggcgtag tgcagccaac
     6601 gccgcagaga gcgtacaacc agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta
     6661 aaccgttgtt caccctcgcg ggtaaacagc cagtccgggc tttgctcatc atccagatga
     6721 ccacctttca ttagcactgc gccacagccc atcgccaaca gcgatcgccc ttgttccagc
     6781 atttcctgtt cggtgcgcgc gtgtggcgcg tcgagcaagg cggcagcttc gggcaagttt
     6841 ggcgttatta atgaaacctg tggcaataat cgactgcgca gcgtagcaac cgccgaaggt
     6901 gaaagcagcg ggtcgccgct ttttgccagc ataacggtgt cgagtaccac gttttggatc
     6961 tgataacgtt gcaaccgttc tgccaccgct tcaacaatat cggtttccgc caacataccg
     7021 attttagtgg tgtcgattcg cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa
     7081 tcaggctcaa tgcgatacac cgactgtacg ccacgggtat tttgcgccac cagtgcagta
     7141 ataactgagc aaccataagc gccaagtgcc gagaaggttt taagatcggc ctgaatcccc
     7201 gcaccaccac tcggatcagt accggcaatc gtcagagcgt taattcgttt catgcctgca
     7261 cctcctgcgt caattgccag agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct
     7321 cgcttctggc gactgcgcgt tctccggctt gtttcatcca gtgacaggca gatgcgacat
     7381 tttccagcgt atcgcctggt aacgcacagc aggcagcgac aaccgccgat aatgcacagc
     7441 cagttcctac cactttggtc attaacggat cgccaccgtg aatgccaacg gcgcgatggc
     7501 catcggtaac ataatccacc tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca
     7561 gtgtttgtgc agcgggtatc gcgttagctg cggcgtcagt ggtatccact ccccgtccac
     7621 cattagcaat gccagctaat gccatgattt ccgaagcatt accacgtatc gctgccggtt
     7681 taaaagataa aagttcatga caaaaatggc ggcgataatc gagcgcacca accgctactg
     7741 gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc aacggcagca cgcatcgcct
     7801 gagcgcgtgg ctgcgtcagt gtgccaacgt taatcaacaa ggcactggcg atagccgcaa
     7861 actgactggc ctcttcggtt tcgataacca tcgctggcga tgcaccgagc gccagcaagg
     7921 tattggcagt aaaggtttgc accacatcgt tggtcatgca gtgcacaaga ggggaatgtt
     7981 ggtgaaaaag gtgtaacgcg tgcgcagatt gcgctgaact cagcaggtcg acttgcatag
     8041 tttgctcctg ccataacgtg aagaagcaat gacctggtgg tccgtgactt ccctacgctg
     8101 gcattatcca gatcaggtga tacgggtatt tctcagcctt cacgcagaag ggcaccccga
     8161 gtcgtttggt tgcgatgata aatcgcagag gaggatggta atgtccagcg cacgcgttgt
     8221 aaacgaggaa aaacggcagt tttacaatcg cgttaataaa ttatttgata tatgaatcca
     8281 gcaccttcag aacgacatcc agatcttctt cacgttttag ctcatccccc tggtgaacga
     8341 tgtgttccgt cagatgacct ttaatcactt cccgcatcag accgtttacc gcgccacgga
     8401 tagcagcaat ctgttgtaaa actgcagcgc attcgtgcgg ctcgtcgagc attttcttga
     8461 gcgccacgac ctggccctga atcttactgg cacgcgcttt cagtttctgt ttatcacgga
     8521 ttgtatgaga catggcaaca cctggttaac aagaatatga aaaatcatag cactattaat
     8581 ctactggggg gtagtatcag gtactggggg ggagtagaat cagattgccg aattaatact
     8641 aagaattatt atcatgaccg aatttacaac tcttcttcag caaggaaacg cctggttctt
     8701 catccccagc gccatcttac ttggtgcgct tcatggcctg gaaccagggc actcaaaaac
     8761 gatgatggcg gcgtttatca tcgccatcaa aggcaccatt aaacaagcgg tgatgctcgg
     8821 actggcagca actatttcgc ataccgcagt ggtctggtta attgcctttg gcgggatggt
     8881 gatcagcaag cgctttactg ctcaatcagc agaaccgtgg ctccagctga tttccgcagt
     8941 gatcattatt ggcaccgcgt tctggatgtt ctggcgtacc tggcgcggcg aacgcaactg
     9001 gctggagaat atgcacgagt atgatcatga acatcatcac cacgatcacg aagatcacca
     9061 cgaccatgga catcatcacc atcacgaaca tggcgagtat caggatgccc atgcacgagc
     9121 ccatgccaat gacattaaac gacgctttga tggtagagag gtcaccaact ggcaaatttt
     9181 gttatttggc ttaaccggtg gtcttatccc ctgtccggca gcaattaccg tgctgttgat
     9241 atgcattcag ttgaaagccc tgacactggg cgcaacactg gtcgtcagtt tcagcattgg
     9301 cctggcgtta acgcttatca ccgtaggcgt tggcgcagca atcagcgttc agcaggtcgc
     9361 aaaacgctgg agcggattta acactctcgc taaacgcgcc ccctattttt ccagtctgtt
     9421 gattggctta gtcggtgtgt atatgggcgt acatggcttc atgggcataa tgcgataact
     9481 caatccttta ggcttgcatg ttatcttcag cccaggatga ttcttaaatc agctatttcc
     9541 gctgactctc tgctcgctaa ggatgctttt agggcatcct ttcatttaca ctttttacga
     9601 aatcatggga tcactaacaa aatatcgctt gtcagttata ttgtatggca ggaaagatat
     9661 gcgactgata ttacagatcc ccaaagtgga gagtttatga ccattaaaaa taagatgttg
     9721 ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg caccagccac cgcgggttcg
     9781 accaatacct cgggaatttc taagtatgag ttaagcagtt tcattgctga ctttaagcat
     9841 ttcaaaccag gcgataccgt cccagagatg taccgcaccg atgagtacaa cattaagcag
     9901 tggcagttac gtaacctgcc cgcgcctgat gccgggacgc actggaccta tatgggtggc
     9961 gcgtacgtgt tgatcagcga caccgacggt aaaatcatta aagcctacga cggtgagatt
    10021 ttttatcatc gctaaaaaaa gccccctcat catgaggggg aaatgcagac accttgttat
    10081 tttttattat tagccacttg ctcgtcttgc ttgttattag tcgtatttca cgttgattaa
    10141 tgcggttgcc tccagtgcgc cagatttaac tttgtttgta tcgtagacgt agtaactggc
    10201 agttatcgga attgaaaccg ttgtatctgt aagttttgac tttgccgcaa tttcttttaa
    10261 atttgtgtat cctttactgc tgttagtgat atttaaaagt gtgactgacg aaccattact
    10321 ttcaagaata aatcctacgc cattatcttc cgcacctttc agtattgtgt tgctgttggt
    10381 taacaagtca gaacttgata aatacacctg cattttccgt gatgtttgat tttgttgacc
    10441 taataaattt ttacaacgca gaataatatc cccttgttta ctatttattg ttgctttgtt
    10501 acctgtggct ggtaattgtg aaacgggaat actgtctaaa gtaattaata aatcgtccgg
    10561 tttgcaggtt gtggttatcg ggttgtatga caatgtcaga ttaaaggaat agacacctgc
    10621 attttgtgcc aatgcatttc tgtagctatt tacacatgca tcccatgact tgccacctaa
    10681 taaacacagt agaaattgta ctgcggcact cccgcgtgtc gatgatgaag cactggataa
    10741 tgtcgcaact ccattaatca tcactgaacc gccgcccccc gcagaaacat tcacttttgc
    10801 attactggcc gcaggtgtca ccgttaccga ataaccactg gtgaaactgg tctgattatt
    10861 tgataacgtg atgttgtttt tattggtcca ttcaaaatgt aaatttaatt taatggtatc
    10921 gttataaagt ccaacaataa tatccgacgg gctcataggg gtgatagtat ttgtactcac
    10981 gcatgacgaa tcattaaaat tatgctcata tgtcgccatt tgtgtttgag taagtgcaaa
    11041 atccccctga acaaccaaat tgtgctgaac agataactca ctataacatg ccgcatagct
    11101 cacaggcata accatcatca ttactataaa taacattata cgaatattca taaccacctg
    11161 actattactg gcaaatatag tttctacttt catcaatctc tttaccgaag gtgattgtgc
    11221 atgaaagtcc ttgttgctta tcaattgcta cattaaccga tggcggtact tcattggtgc
    11281 gaataaataa ctggcttccc tgaccgacaa cgccaatatt atgaccatgg atatcattga
    11341 cttcataacc aaacattaat ggttgcccat ccgctcttaa cgcttttata aaccagggct
    11401 tgcgctgatc ggtatcaaaa ttaaccagta caaccgcgcc gcgataaggg gcggcaattt
    11461 tccggttgcc acgtaattct gcttcgctat cgctttgcga cacatccagc atcaggtgat
    11521 tttcccgata aggtgtcatt ccgtcgtata ccaccactcc attacggttt gttgtgcgat
    11581 atttttgccc attgacataa gcatctttaa ttcctggcgc attcatcaca gcaaacgttt
    11641 cggaaagacg gttcgccaga ttaacgcccc ccgaccaggc gacaatgccc cctgaaacac
    11701 tggctccagc ctgtcgataa gtactcgact gactataact gccattcact gtcgcaaccg
    11761 gcgcgttcca ggtcaaattc gccccagctg tcgtttcatt tccctgattc tgataactca
    11821 ggttgacccc atagttaaac tgatcccggc ttccaacggt tcctgataat cccgtattat
    11881 ttgaggcaaa cccctgatca tcaaacgtcg ttgagttaga catatatatt tgccgacgag
    11941 gcgtcgtaac gtcatcaccc caatcaaagg gaatcgatat aaaaatatta aaacgtttct
    12001 cttcatgatg attctcgtca tacgcctggc ttgccgcgag gatatagctt atccgccgcc
    12061 agttattgga ataactcaac tgataatcct tactgctgcc gctacgcccc cagtaatctc
    12121 gccataacgt acttaaggac acagaccccc agccttctgg caatgactgg ctcatattgg
    12181 cagagaagct atttttacgg ccaaaatcgt tctggtaata atcggcaatg tcatagacat
    12241 cgttttcatc acggcgataa ttatctttat tgtttgccca aacgtgatca ttaaatgtcc
    12301 ggtaatcacg cgatgaataa cgccaggccg ccagaccaaa acgcgtcgac gtttggctca
    12361 caaatttgtt gtaggcaatt tgataacttt gcccgtcaaa cacatcgccg ttgtcttgtt
    12421 tactatgcga cttcgtggca tcgacggaaa tggcaccaaa gcgtgtattc cagccagtcc
    12481 ccaaagtaaa cgcgtaataa ttattcgcga ccatcgagcc accatacagc gtcaataaat
    12541 tattaaaacc atactgataa cccgcctgga caaaatcact ttgtttgctc gccccttcaa
    12601 tatggctacg acccgccgca aaatcatatt tcgatacgcc gggttgcagc atatttggca
    12661 ctgctgcata aggcaccaga taggtggtta ccgagccgtc cgcctctttc acgctgacat
    12721 caagatctgc tccaccaccg gccaactgca aatctgtaat cgcgaacggg ccaggaggaa
    12781 cctctttctg ataaaccaca aaaccgttct gttcaatagt taccagcgca ttactctgag
    12841 caatcccctg cacccgtggc gtaaaatttt gtttcgagtt aggcaacatc tgcatatcac
    12901 gaaacaaccg cacaccgctg aagcgaacag aatcaaaaat atcgcttgat gtgtacatat
    12961 cacccacgcg aagcgtgccg agaagttggg caaatccacg ttccagatac agggtattgc
    13021 ttttccacac ccctggattg ttatttgttt tactgaaact ggcatcagaa tgcagttgcc
    13081 accccagtaa atttaacccg ctgttaaaac gtacatatgt actcttacta ttacccgacg
    13141 ctttatagtc actgtaatac tgactcacat aataagaggt ataaaacgca ttaatacccc
    13201 gctcccagtt ttccggcgga acatagccac tttccagttc ttccacccag gcctgcggga
    13261 cactgaaatc gagacgaaaa accccgatat cccaggtata gctcctaccc tgaacaagtt
    13321 gctcaaatgt taaacattgc ttaccgctgg cgaagttatc gctattaatg cctaaccgct
    13381 tgataacttc tattgataaa catgtttctt gcgggttgtc tttaacaata atctcatatt
    13441 tcccgcgcca ttgcttattg acataaatat cgatgtcata ctgccccggt aagggttgat
    13501 tatcatcaag acgaatattt gcaacctgct ggtctttcat tccacctatc ataaaatggg
    13561 tatcaaaggt ttcttcagct gcataagctt caatgccgag caataacgct acgattgctg
    13621 atgcaagtgg ggtcattctc aacataggca gctcctgcaa ttaaattttg tcactaatat
    13681 agttgccgtg gtcatcgata atggtcagat accagttatt tgcattatta cttttgacat
    13741 taacactctg actttctaag ggggcaatca taatagtttc ataattgact ttaacattat
    13801 tagctttgac atccgaaatc gtcacccaat tagctgagtc attttttatc accaatccat
    13861 tgccactgcg atttaccagt aactttttaa aggtcgcttt atttaccgga gcaatacccg
    13921 ctggccggta aaacaactta attctgtttt gcatcgcaaa cttcagtgca tttttgcctt
    13981 cttgctctgg actatttggt ggaatatcca gaacattcag ataaaaaatg ctttctttat
    14041 tagtgggcag tttattcggc ataattttga tttttacttg ctgcccggaa tttgccccta
    14101 tttttgccac tggtggcgtt aacatgaaag gaacctgaat tttttctggt ggtaacgacg
    14161 tatcgccatc atcaatccac gcctgcagca gcgaagaacg gtttccctgg ttcatcaact
    14221 gcaccataac ttctttattt tctgccgggt aaataatgcg cgtcccgtag ataacaattc
    14281 cggcatgtgc aggaaggacc atagaaaaaa tgagtaaaga taataatcct ttcatcttaa
    14341 tgcctcttaa attaaaaagc cgccatgggg atagcggcca tgtttattac ctggagaaac
    14401 ttacaatttt atttattaat cctgcgccac acgcataacc agagaagata acacatcacc
    14461 tgattgcact gagttatctg tcagtttcag gtattttgct ttaaacggaa tagcatattg
    14521 accttccgct gttttataac tctcaatgtt aaaaggtgca gaaacatcca ggacatcgct
    14581 gttatcacct aaatagagat agtaacccac ggtatcgttg ctactggcta tcgccttatc
    14641 gttaccggtt attttatcgt tggtaatcaa cgagagtttt acaccagcaa cttctttcgg
    14701 gcagctaatt tttaacatgc tgctggttac accgactgca tcatcatagg tagaatattt
    14761 agctttacct tcaaagacag tggttggtac agtcggcata ttaatactta ctgaatcacc
    14821 acccgcttcg aatttacacg gagattctgc aatattcccc ttaatagtta atgttccggt
    14881 atcaacgtct gcagcaaata ctccagcgct cataaaaaaa gaagaaaaga cagcggcagc
    14941 aataattgaa cgtttcataa tatgttcctt aataaaattt atttttggtt gttaagagtc
    15001 tgtatctctt ggaaacaaat ttctcacatc aaaaaagttg ccgcaatgta aacacattgt
    15061 tttattgatt taaaacaaa
//
LOCUS       DAZGCS010000114        15014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGCS010000114 DAZGCS010000000
VERSION     DAZGCS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.116"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..374
                     /gene="yjbE"
                     /locus_tag="ACHK01_003963"
     CDS             132..374
                     /gene="yjbE"
                     /locus_tag="ACHK01_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO8098833.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            488..1126
                     /locus_tag="ACHK01_003964"
     CDS             488..1126
                     /locus_tag="ACHK01_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO8098834.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1123..1860
                     /locus_tag="ACHK01_003965"
     CDS             1123..1860
                     /locus_tag="ACHK01_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8098835.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAASRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1860..3956
                     /locus_tag="ACHK01_003966"
     CDS             1860..3956
                     /locus_tag="ACHK01_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8098836.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4003..4281)
                     /locus_tag="ACHK01_003967"
     CDS             complement(4003..4281)
                     /locus_tag="ACHK01_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098837.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4297..4425
                     /locus_tag="ACHK01_003968"
     CDS             4297..4425
                     /locus_tag="ACHK01_003968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098838.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4551..4961
                     /gene="psiE"
                     /locus_tag="ACHK01_003969"
     CDS             4551..4961
                     /gene="psiE"
                     /locus_tag="ACHK01_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO8098839.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5005..6480)
                     /gene="xylE"
                     /locus_tag="ACHK01_003970"
     CDS             complement(5005..6480)
                     /gene="xylE"
                     /locus_tag="ACHK01_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO8098840.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6852..7742)
                     /gene="malG"
                     /locus_tag="ACHK01_003971"
     CDS             complement(6852..7742)
                     /gene="malG"
                     /locus_tag="ACHK01_003971"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO8098841.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7757..9301)
                     /gene="malF"
                     /locus_tag="ACHK01_003972"
     CDS             complement(7757..9301)
                     /gene="malF"
                     /locus_tag="ACHK01_003972"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO8098842.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9455..10645)
                     /gene="malE"
                     /locus_tag="ACHK01_003973"
     CDS             complement(9455..10645)
                     /gene="malE"
                     /locus_tag="ACHK01_003973"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO8098843.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11010..12125
                     /gene="malK"
                     /locus_tag="ACHK01_003974"
     CDS             11010..12125
                     /gene="malK"
                     /locus_tag="ACHK01_003974"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO8098844.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12197..13537
                     /gene="lamB"
                     /locus_tag="ACHK01_003975"
     CDS             12197..13537
                     /gene="lamB"
                     /locus_tag="ACHK01_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO8098845.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            13780..14700
                     /gene="malM"
                     /locus_tag="ACHK01_003976"
     CDS             13780..14700
                     /gene="malM"
                     /locus_tag="ACHK01_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO8098846.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3715 a         3861 c         3944 g         3494 t
ORIGIN      
        1 tcattccaat tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta
       61 gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa
      121 gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga
      181 cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg
      241 tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg
      301 cgctcgcggc aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca
      361 ccagtaccca gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct
      421 tgaagtatga agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag
      481 aagagtcgtg aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc
      541 cacgactaaa gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca
      601 gctgacggac gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg
      661 cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac
      721 tcaggatcaa gccactcttg tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg
      781 cgacaacctg attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat
      841 tgttgatggt gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta
      901 cgccaccgca cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga
      961 tgaaaccccg gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa
     1021 ccgctactgg atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga
     1081 ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt
     1141 gtcgcattga ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta
     1201 ttcagcaatg gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg
     1261 gtaggccagc cgcggctggc aaacagttgg tggcctggtg cggtgattag cgaagagctg
     1321 gcaacggcgg cagcatcgcg gcagcagcag gcgttgctga cacggctggc agaactggct
     1381 gcagattcca gcgccgacga tgccgccgcg attaacgcct tacgccagca aattcaggcg
     1441 ttgaaagtga cgggcaggca aaaaatcaat cttgatcctg atatcgtccg cgttgccgaa
     1501 cgcggtaacc caccgttgca gggcaactac acgctgtggg tcgggccacc accgtccacg
     1561 gtcacgctgt tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgcgac
     1621 gtggcgagct atctctccgg gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg
     1681 gtggtttacc cggacggacg cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac
     1741 gtagagccga tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag
     1801 acgcctgatg cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa
     1861 tgaaaaaaag acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg
     1921 aaacatatcc tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac
     1981 aaacgcccac cgcgcgcatg gcgcgggaag gggagttgag cctgaactat cgagataacg
     2041 atcagtaccg ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc
     2101 gctacaccga cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa
     2161 cgtataaaga taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc
     2221 cgcaagtggc ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc
     2281 ttgttgccag caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc
     2341 tgggcaccag cggtaatgtg aaaaatccgc tctgttcagc cagtgataaa tattgctatc
     2401 gcgataacag ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg
     2461 cctcactgtt tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg
     2521 agtatgaagg caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt
     2581 ttaacgtcgg tgcgatttat cgcgttaccg attgggccga cgttaacctt agctatgaac
     2641 gtggcaacac ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt
     2701 cctacatcga taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc
     2761 attcggtggt ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc
     2821 agatccaggc gaaaggcgat acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt
     2881 cgcgcgaagg gatcatccgt gctaatcgga tcgtgatgaa tgatctgccg gatgggatca
     2941 aaacgatccg cgttacggaa aatcgcctta acatgccgca ggtgacgacg gaaaccgatg
     3001 tcgccagcct gaaaaatcat ctcgccggag agccgttggg ccacgaaacg acgctggcgc
     3061 aaaaacgcgt cgagccagtg gttccgcagt ccaccgagca gggctggtat atcgacaaat
     3121 cacgctttga tttccatatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact
     3181 tttacatgta tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctac
     3241 tgaccaccgg cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacca
     3301 atccaccgca ggactcgcac ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga
     3361 acgatgtcta tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct
     3421 acggtcaggt ctacggtggt tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt
     3481 tgtatcgccc gctggacagc aactgggcgt ttggtctgga tgccaactac gttaaacagc
     3541 gcgactggcg tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac
     3601 atctgaccgc ctactggacg ccatctttcg ctcaggatgt gttagttaaa gccagcgtcg
     3661 ggcagtatct ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg
     3721 gcgtggtggt gggtggctat gccacgatca ctaatgtttc aaaagaggag tacggcgaag
     3781 gggacttcac caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga
     3841 cacgcagccg tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg
     3901 ggcgtaagtt ccagttgtat gacatgacca gcgaccgtag cgtcaatttc cgctaagtca
     3961 tgggaaaggt gccagttttc gcaatcactg gcaccctcat tcttaatggc ctgttgagcc
     4021 atcaggaacg cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt
     4081 cttcctgtcc gtcgatatcg gttgcagatc tggttgcctg agcgactggg ccatcattgg
     4141 gctggagaca ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata
     4201 agcaggaaga acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac
     4261 ctttatcagg ttacgtttca tttgttccag aggaacatgg tcgatttttc gcgcattgct
     4321 ggtggctggg aatcacctga atgggtgatt tttgaattac cggctttggt gcggtttgtc
     4381 ttgccggatg cgccgccagg cgcggcttat ccggcctacg ggtaggtata tccggctttg
     4441 gtggaggcgc gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt
     4501 cacatttttg cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac
     4561 tctctcgtcc gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc
     4621 tgttgtgcct cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggcag
     4681 atgtgctgtt cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt
     4741 actttctcta tttcgaattt atcgcgctga ttgtgaaata ctttcagtcc ggttttcact
     4801 tcccgctacg ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg
     4861 atcataaatc gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca
     4921 ctctgtggtt gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact
     4981 ggcgcggcgt gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc
     5041 cggttcccag agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt
     5101 ccacataaat agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt
     5161 gtggaaatgg gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa
     5221 gtagttcgcc agccactggg ccgccaccgc gattgccagc gctttaccac gaatggcatt
     5281 cgggaagatt tccgacagca gaacccagca taccggaccc caggacatgg caaaggcggc
     5341 aacatagaac agcatcgaca gtagcgccac aatacccggt gcctgagtgt aaaacgcggt
     5401 accgaggcta aacataccga ttgccattcc gagcgcgccg ataatttgca gtggcttacg
     5461 accaaattta tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac
     5521 aataatggtc tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg
     5581 cgcgtagtac aacaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc
     5641 gattacaatc acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc
     5701 cagggagtgt ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg
     5761 caggatactt tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg
     5821 cacggtatac agcagcatta agaacagcag tgcagggata cattccgagg caaacatata
     5881 acgccagccg tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac
     5941 gcagtaaact aaaagttgcc cgaaaataat cgcaaactgg ttaaatgaaa ccagtttccc
     6001 gcgaatatga gctggagcca gttccgcaat atacattggt gaaagcattg aggctaaacc
     6061 aacgccaata ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata
     6121 aacaggcact gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc
     6181 tacaccagaa ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg
     6241 gttactgcaa taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggctac
     6301 gcaaaaccct aacagggagt tggcagcgga ttcatttaag ttttgtggag caacaaagac
     6361 ggtattgagt gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc
     6421 acctaatgta gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat
     6481 tcagacctgc cttagatcat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg
     6541 cagttaaggc aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta
     6601 attgtgataa ttatccaaaa ttgataaaaa caatactatt gccgtgattc agagcacgaa
     6661 agagaattat cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatgggaa
     6721 tattaaccgg cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc
     6781 cgaataatag tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca
     6841 gaacaacatc tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga
     6901 gccagcaaga agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca
     6961 ccccacaggt agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagcta
     7021 tttacgtcac gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac
     7081 aggatgaaca ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc
     7141 tgccacggtg tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg
     7201 aaatagcctt tgatggtcca gacatgcagc gcaatcccac ccagatacgc gaaaattacg
     7261 ccgccatgag tattcagacc aatgaatgga atgtactcac ccagacgatc aaacaacgca
     7321 tacaacgcga ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc
     7381 agcgtcgctt tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc
     7441 gccacaatgc caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt
     7501 accgggaatg gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc
     7561 agtttccagt gatcccagga gatttgctcc gggatcaggc taccggtcgc gaagtttccc
     7621 tgacgcagcg agatagcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt
     7681 agcagtagca ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc
     7741 atttttgtta tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca
     7801 gcgcacccac cagcaggaag atcagcgtgg caattgctgc cgccagaccg aagtcctgac
     7861 ccccgccgcc ttcaaaagcg atacggtagg tgtagttaac aagcaggtcg gtataaccgg
     7921 ctggcgtggt cgtgccaaga cgatccgggc cgccgttggt taacagctga atcagcacga
     7981 agttgttaaa gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca
     8041 gcggcagcgt aatcttaaag aagttctgga acggacctgc gccatccatt gctgaggctt
     8101 catacaaatc gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat
     8161 aacccagcca ggtattgacg ataatcagca tcgtgcgggc ggtggtcgga tcgctgaacc
     8221 aggcgggctt cacgccaaac agcgcgctca acatcatgtt gatttcaccg aagctctggt
     8281 taaacaatcc tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca
     8341 gcaggacgcg atagaccgct ttgccgcgca acgcttccca ctgcaccaga catgccagaa
     8401 ccatgccgac cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga
     8461 aaatggcgag gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc
     8521 agccggtggt cacggtgtaa ccggggctta gcttttcatc accccagttg ccgtcggcgg
     8581 taatggactg gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat
     8641 tcgtcaacgt gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca
     8701 gggagctcat catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct
     8761 gacgattctg ggtaatcacg cgcagattcg cgcgttcgcc ttcgggctgg gcggtcgttt
     8821 ctttcagttg cagtttttgc tcgccgccaa atttaaacgc gtcggagagg taatttttgc
     8881 cggtttcgcc gtcgctgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt
     8941 tataggtttt gcctgcttgc caggagcgat ctaacaacac ttcctgcgca cgttcaaaag
     9001 tcagctggtt agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga
     9061 agaggacgaa taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt
     9121 tacgattggc gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca
     9181 ggtattcccc ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac
     9241 ctagcactga ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca
     9301 tggggttctt cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc
     9361 ctgatgcgac actgatgcgt cttatcaggc atacatgcgt ttcagttttg taggccggac
     9421 aaggcgttca cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt
     9481 ctttcagggc ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca
     9541 cggcatacca gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt
     9601 tttccatggt ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg
     9661 ccactgcacc cagcggtttg tctttattga ccgcttccag accttcgtca gtcagcagat
     9721 agttttcgag gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc
     9781 tcagcacgcc aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac
     9841 cataattcac tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg
     9901 ctgtttcgcc tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt
     9961 gtttgttttt aataaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca
    10021 cgcccacgtc tttaatatcg tacttgccgt tttcatactt gaacgcataa cccccgtcag
    10081 cagcaatcag cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac
    10141 ctttcgcttt cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg
    10201 gcagcagatc tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca
    10261 gcttgccgtt gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt
    10321 tgtccggggt gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgtg
    10381 cccagaagat aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca
    10441 gtttatccgg atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga
    10501 cttcagcgag accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt
    10561 cgattttggc gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga
    10621 tgcgtgcacc tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa
    10681 acacctaaac ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta
    10741 cgccccaccg ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc
    10801 acataaaatc gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt
    10861 tagttcacag aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg
    10921 tggaggattt aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg
    10981 atgacaggtt gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga
    11041 aagcctgggg cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat
    11101 tcgtggtgtt tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg
    11161 ggcttgagac gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacacac
    11221 cgccagcaga acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt
    11281 cagtagcaga aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta
    11341 accaacgcgt taaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac
    11401 cgaaagcgct ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg
    11461 agccaagcgt atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc
    11521 aaatgcgtat cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca
    11581 cccacgatca ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc
    11641 gcgtggcgca ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg
    11701 ccggatttat cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa
    11761 tcgatcaagt gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg
    11821 aaagccgtga tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac
    11881 tgccgagtga tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg
    11941 gcaacgaaac ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc
    12001 agaacgacgt ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc
    12061 gttgccatct gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg
    12121 tttaagcacc ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa
    12181 tgactcagga gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc
    12241 agcgggcgta atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg
    12301 tattggctgg acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag
    12361 taaataccgt cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt
    12421 gtggaaagag ggcgataaga gcttctattt cgacactaat gtggcctatt ccgtcgcaca
    12481 acaaaatgac tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa
    12541 tctgatcgaa tggctgccag gctccaccat ctgggctggt aagcgcttct accaacgtca
    12601 tgacgttcat atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga
    12661 aaacatcgat gttggcttcg gtaaactctc tctggcagta acccgctcct ctgaagcagg
    12721 tggttcttcc tctttcgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga
    12781 cgttttcgat gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg
    12841 tgtcgactac ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa
    12901 agacggctgg ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt
    12961 tgttgttcag tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc
    13021 tggtgttgca tatgttgacg aaaaattttc ctatgacatt aacaacaacg gtcacatgct
    13081 gcgtatcctc gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg
    13141 tatgtaccag gatatcaact gggataacga caacggcacc aagtggtgga ccgtcggtat
    13201 tcgcccgatg tataagtgga cgccgatcat gagtaccctg atggaaatcg gctacgacaa
    13261 cgttaaatct caacgtactg gtgataccaa caaccagtac aaaatcacac ttgctcaaca
    13321 atggcaggct ggcgacagca tctggtcgcg tccggctatt cgtgtttttg ccacctacgc
    13381 caagtgggat gagaaatggg gttacgatta caccgacaaa gctaaaggta ctccgaacta
    13441 cggcaaagcc gttccagcta acttcgaagg cggcagcttc ggtcgtggcg acagcgacga
    13501 gtggaccttc ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag
    13561 gtgcttgcgc cgcatccggc atccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc
    13621 tacaacggct gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca
    13681 ggttgtcatt atctgaaagg ggcgcaagcc cctctgatta tcgggtttag cgcgctattg
    13741 cctggctacc gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc
    13801 atcgccctct gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat
    13861 gttaactacg taccgcaaaa caccagcgac gcgccagcca ttccatctgc cgcgctgcaa
    13921 caactcacct ggacaccagt cgatcaatct aaaacccaga ccacccaact ggcgaccggc
    13981 ggccaacaac tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca
    14041 aacattggcg aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgtattt
    14101 gcgccgaacg tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt
    14161 tatttcacct accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc
    14221 ctgacaccgg cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat
    14281 ctccagcaga cgacccaact gctcgacccg gctaaagcct acgccaaagg cgtgggtaac
    14341 tcgatcccgg atattcccga tccggttgct cgtcatacca gcgatggctt actgaaactg
    14401 aaagtcaaaa cgaactccag ctccagcgtg ttggtaggac cactatttgg ttcttccgct
    14461 ccagctccgg ttacggtagg caacacggcg gcaccagctg tggctgcatc cgctccggca
    14521 ccggtgaaga aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc
    14581 aaaaacgctg tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa
    14641 cgcctgggat cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa
    14701 ttacgcccca cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta
    14761 tgcttccttc ttgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa
    14821 tgtccattat ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca
    14881 ttcaggctac ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat
    14941 atcccaaatt ctggcggcaa taactctttg atgaaacatg atgtggtgca aggaaataat
    15001 atagtagatc ttga
//
LOCUS       DAZGCS010000115        14850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGCS010000115 DAZGCS010000000
VERSION     DAZGCS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.117"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHK01_003977"
     CDS             complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHK01_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO8098847.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            947..1030
                     /gene="yqgH"
                     /locus_tag="ACHK01_003978"
     CDS             947..1030
                     /gene="yqgH"
                     /locus_tag="ACHK01_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO8098848.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="ACHK01_003979"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="ACHK01_003979"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO8098849.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCQLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHK01_003980"
     CDS             complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHK01_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO8098850.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHK01_003981"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHK01_003981"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO8098851.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHK01_003982"
     CDS             complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHK01_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO8098852.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHK01_003983"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHK01_003983"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO8098853.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEMMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="ACHK01_003984"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="ACHK01_003984"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO8098854.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /gene="yggL"
                     /locus_tag="ACHK01_003985"
     CDS             8213..8539
                     /gene="yggL"
                     /locus_tag="ACHK01_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO8098855.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8562..8702
                     /locus_tag="ACHK01_003986"
     CDS             8562..8702
                     /locus_tag="ACHK01_003986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098856.1"
                     /translation="MQNCLMRYAYQAWKDARSSRRDKVFTPHPAWKTRTLLSIWGQQM
                     LA"
     gene            8723..9442
                     /gene="yggN"
                     /locus_tag="ACHK01_003987"
     CDS             8723..9442
                     /gene="yggN"
                     /locus_tag="ACHK01_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO8098857.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="ACHK01_003988"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="ACHK01_003988"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO8098858.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDLATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAQALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11788
                     /gene="yggM"
                     /locus_tag="ACHK01_003989"
     CDS             10781..11788
                     /gene="yggM"
                     /locus_tag="ACHK01_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO8098859.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11844..13145)
                     /locus_tag="ACHK01_003990"
     CDS             complement(11844..13145)
                     /locus_tag="ACHK01_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO8098860.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13145..13648)
                     /locus_tag="ACHK01_003991"
     CDS             complement(13145..13648)
                     /locus_tag="ACHK01_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO8098861.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13693..14679)
                     /locus_tag="ACHK01_003992"
     CDS             complement(13693..14679)
                     /locus_tag="ACHK01_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO8098862.1"
                     /translation="MKMRSFTRSLVCASLLTLVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETATKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3947 a         3537 c         3745 g         3621 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgttggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga tgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttaac tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgctt tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaactggta tcccgacttt cttctcatcg tcgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttttttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cgggggcgac gccggtctat ctggaagctt cacgcaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga gctgccgcgc ccgtttcgcc tggcgattat tcagctggga
     2041 acctatgacg gaactgtcta taacgcccgt caggtgatcg ataccgttgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatccc gatgatggcg
     2161 gatagctcgc cgctgctatt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 tcggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataatgc ctttatgctc
     2341 catgcttcta ccagcccgtt ctacccgctg tttgccgcgc tggacgttaa tgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtcagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatct gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ctgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtagcgat gttggcgcag tttgaacagc atattgagga tgactcgccg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatg gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gagctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaagacc gctgcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggaaccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaatgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcggtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg taccatgtta atggtgacgc tgtagatgat
     5401 acgcagcccg ttactgcggc tttgcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatagtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagt tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctcaagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgct
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaattgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagctgcg
     6481 tcaggttatc ggcagcaatc tgccgttgcg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc
     6841 ataatttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgctcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc tttatgcaga ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc atttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgtttttcgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg agtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga
     8341 tgattttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctgg aaagatgcac gatcgagtag gcgggataag gtgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct
     8701 gattttttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg
     9121 cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacaccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacctagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagttggcat cgtttataac tacgctaacg catccgatct tccggctcaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggca ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    11701 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca
    11761 actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa
    11821 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    11881 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc
    11941 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    12001 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    12061 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    12121 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    12181 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    12241 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    12301 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    12361 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    12421 gtaaaaatac cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag
    12481 gcatccagaa acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca
    12541 cggcgtttag caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc
    12601 aaatatcccg ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca
    12661 attaacacgt ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg
    12721 gtagaaaatg ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg
    12781 gcagcagctg ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg
    12841 ttcacatgac caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa
    12901 cgtatcgcaa taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc
    12961 aatagggcaa agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata
    13021 tcccagggaa acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg
    13081 ggcacaccaa taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta
    13141 tccattaact ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac
    13201 acaaggcata aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca
    13261 tcggtatttg catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca
    13321 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac
    13381 taagaagtaa ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc
    13441 tctgcgcgcc gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg
    13501 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
    13561 ctacgcatac aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta
    13621 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac
    13681 tggcccggaa tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg
    13741 atcttttttg aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac
    13801 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
    13861 ccacaatttt tgttgataag cttcggattt cttcgttgct gtttcgagga tttgttgctg
    13921 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
    13981 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg
    14041 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
    14101 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
    14161 ctgaacacga attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata
    14221 gaaactgcgt gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
    14281 gtccataatt tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt
    14341 aaataagaac ggcaaattat agatagcata aatattatca aaagactcca gatcactggc
    14401 ggaacctttg gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt
    14461 acccatctgg ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac
    14521 ctctttcgcc agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc
    14581 taactttaac gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag tcagcaagga
    14641 tgcgcagact aatgagcggg taaatgacct cattttcata aaaaaactcc ctgttgaagg
    14701 atggaaattg gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac
    14761 ctgtctgaca acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta
    14821 aatttgtgcg ccaggttgta catgtttata
//
LOCUS       DAZGCS010000116        14477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGCS010000116 DAZGCS010000000
VERSION     DAZGCS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.118"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..592
                     /locus_tag="ACHK01_003993"
     CDS             26..592
                     /locus_tag="ACHK01_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8098863.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHK01_003994"
                     /pseudo
     CDS             643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHK01_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHK01_003995"
     CDS             3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHK01_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8098864.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHK01_003996"
     CDS             3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHK01_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8098865.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5058..6104
                     /gene="abrB"
                     /locus_tag="ACHK01_003997"
     CDS             5058..6104
                     /gene="abrB"
                     /locus_tag="ACHK01_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO8098866.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHK01_003998"
     CDS             complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHK01_003998"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO8098867.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHK01_003999"
     CDS             complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHK01_003999"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO8098868.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHK01_004000"
     CDS             complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHK01_004000"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO8098869.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHK01_004001"
     CDS             complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHK01_004001"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO8098870.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHK01_004002"
     CDS             complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHK01_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO8098871.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHK01_004003"
     CDS             10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHK01_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO8098872.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHK01_004004"
     CDS             complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHK01_004004"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO8098873.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHK01_004005"
     CDS             complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHK01_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO8098874.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13869..14477)
                     /locus_tag="ACHK01_004006"
                     /pseudo
     CDS             complement(13869..14477)
                     /locus_tag="ACHK01_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3499 a         3868 c         3500 g         3610 t
ORIGIN      
        1 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactga
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg
     1861 cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga
     2461 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccaa cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac
     4441 tgcccaaaac ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4741 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4801 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4861 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4921 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4981 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5041 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5101 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5161 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5221 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5281 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5341 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5401 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5461 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggcagctg
     5521 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5581 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5641 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     5701 cagtgctcca gtctggtcag ctcatcacca tcgaactccc ggaatggttg ctggcgatgg
     5761 cgtatatggc aattggttgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5821 cattgcgccc actgccacaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg
     5881 gtatggcgtg ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca
     5941 ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6001 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     6061 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac
     6121 accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc
     6181 acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6241 catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga
     6301 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     6361 gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca
     6421 gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     6481 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6541 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6601 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6661 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     6721 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6781 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6841 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6901 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     6961 tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac
     7021 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7081 catctccagc gtttgcgcca acgcctgttc ttcgttttca atcaacgcgc ctcgctgact
     7141 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7201 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat
     7261 caatgccgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7321 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7381 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7441 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     7501 aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatgattt gcgcatcgcc
     7561 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     7621 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta
     7681 attgttcgaa ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7741 ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa
     7801 tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     7861 gctgcccgtt atgcggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc
     7921 gatcggtgat gcgttttaaa atttgcccct gtaagcgata gcccattcgg ttactttgcg
     7981 agctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     8041 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     8101 cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     8161 ggccttccag cccgccaatc cccactttga gatcggtgct gcatgacccc attaccggcg
     8221 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     8281 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     8341 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     8401 ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca
     8461 ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata cccgactggc
     8521 gaaaaccgtg acgaccgcca tcttgcacag tggtatacat gcccgcacga ataatcttca
     8581 gcatactccc tccttttgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     8641 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccacccacc
     8701 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8761 aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8821 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8881 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     8941 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     9001 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     9061 acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     9121 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     9181 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     9241 tccctctgat tattcgttat gcaggattag gtatatcaat aaaggtcaca tcaagatcgg
     9301 tattttcatt cagccactca ctcaatgcgc gaataccacc gcgttcagtg gcatggtgac
     9361 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     9421 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     9481 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     9541 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     9601 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9661 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     9721 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9781 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9841 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9901 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9961 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
    10021 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
    10081 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
    10141 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
    10201 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
    10261 tggaattatt atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct
    10321 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
    10381 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
    10441 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
    10501 ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga
    10561 gatccatccg tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt
    10621 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10681 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc
    10741 ctgtggttac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10801 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10861 cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg gatggctgct
    10921 ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag agtggtcggt
    10981 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    11041 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    11101 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
    11161 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    11221 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    11281 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc
    11341 cgggttctgc attctgacct taagcgcccg ctggtcctca atgtatggtc actcttctct
    11401 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
    11461 tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat taaccggcat
    11521 ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg ccgatcagac
    11581 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    11641 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11701 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggaatctt aataggccag
    11761 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
    11821 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
    11881 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    11941 gcgcagctcc ggtaaccatc ggcggataaa ctcgccctca cggtcaaatt tctcgccctg
    12001 agttatcgga ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca
    12061 ctgccagcca ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    12121 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
    12181 tagccggtta tgcatccagc cagtactgtt aagctgacgc atagcggcat caacaatcgg
    12241 gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac
    12301 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
    12361 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    12421 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    12481 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
    12541 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    12601 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    12661 cgcgggctct atcgatccgc tactacgaac ttttggcgca gcgacacact ccggcatccc
    12721 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    12781 attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
    12841 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
    12901 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    12961 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
    13021 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    13081 cgtcgcccac tggcgtggtg tagcgatata taacgccagc acgcgtgcag acgaattgcg
    13141 gcaggcggca gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag
    13201 atgggtaatc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
    13261 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
    13321 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc
    13381 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg
    13441 gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg
    13501 ttgtaaaaat catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
    13561 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
    13621 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata
    13681 cggattaggg tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca
    13741 ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca
    13801 aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc
    13861 cctttctatt caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga
    13921 cgggtttctt ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta
    13981 tttaattctt tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct
    14041 tttacagcga ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg
    14101 caacccatcg catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttca
    14161 gggatagctg tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg
    14221 tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa
    14281 gagtgccgga gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa
    14341 tcacgcatcc agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact
    14401 ctggcaatag tatcgtgaac aggaatacca ttttcaaaat caccatattg cttcaagaaa
    14461 tcgagatgtg tttcccc
//
LOCUS       DAZGCS010000117        14420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGCS010000117 DAZGCS010000000
VERSION     DAZGCS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.119"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..806)
                     /gene="artM"
                     /locus_tag="ACHK01_004007"
     CDS             complement(138..806)
                     /gene="artM"
                     /locus_tag="ACHK01_004007"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO8098875.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(806..1522)
                     /gene="artQ"
                     /locus_tag="ACHK01_004008"
     CDS             complement(806..1522)
                     /gene="artQ"
                     /locus_tag="ACHK01_004008"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO8098876.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1529..2260)
                     /gene="artI"
                     /locus_tag="ACHK01_004009"
     CDS             complement(1529..2260)
                     /gene="artI"
                     /locus_tag="ACHK01_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO8098877.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2278..3006)
                     /gene="artP"
                     /locus_tag="ACHK01_004010"
     CDS             complement(2278..3006)
                     /gene="artP"
                     /locus_tag="ACHK01_004010"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO8098878.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3224..3739)
                     /gene="ybjP"
                     /locus_tag="ACHK01_004011"
     CDS             complement(3224..3739)
                     /gene="ybjP"
                     /locus_tag="ACHK01_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8098879.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3865..4188
                     /gene="ybjQ"
                     /locus_tag="ACHK01_004012"
     CDS             3865..4188
                     /gene="ybjQ"
                     /locus_tag="ACHK01_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO8098880.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4185..5015
                     /gene="amiD"
                     /locus_tag="ACHK01_004013"
     CDS             4185..5015
                     /gene="amiD"
                     /locus_tag="ACHK01_004013"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO8098881.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQIDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5012..6025)
                     /gene="ybjS"
                     /locus_tag="ACHK01_004014"
     CDS             complement(5012..6025)
                     /gene="ybjS"
                     /locus_tag="ACHK01_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8098882.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6124..7554)
                     /gene="ybjT"
                     /locus_tag="ACHK01_004015"
     CDS             complement(6124..7554)
                     /gene="ybjT"
                     /locus_tag="ACHK01_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO8098883.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7565..8566)
                     /gene="ltaE"
                     /locus_tag="ACHK01_004016"
     CDS             complement(7565..8566)
                     /gene="ltaE"
                     /locus_tag="ACHK01_004016"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO8098884.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8603..10321)
                     /gene="poxB"
                     /locus_tag="ACHK01_004017"
     CDS             complement(8603..10321)
                     /gene="poxB"
                     /locus_tag="ACHK01_004017"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO8098885.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10454..11422)
                     /gene="hcr"
                     /locus_tag="ACHK01_004018"
     CDS             complement(10454..11422)
                     /gene="hcr"
                     /locus_tag="ACHK01_004018"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO8098886.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11434..13086)
                     /gene="hcp"
                     /locus_tag="ACHK01_004019"
                     /gene_synonym="priS"
     CDS             complement(11434..13086)
                     /gene="hcp"
                     /locus_tag="ACHK01_004019"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO8098887.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13230..14129)
                     /gene="lysO"
                     /locus_tag="ACHK01_004020"
     CDS             complement(13230..14129)
                     /gene="lysO"
                     /locus_tag="ACHK01_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO8098888.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3488 a         3854 c         3810 g         3268 t
ORIGIN      
        1 tataagaatt tatgcactat gtaggccgga taaggcgtcc acgccgcatc cggcacaggc
       61 accgtgctga tgtctgatgc gacgctggcg cgtcttatca gacctacaaa cccccccggc
      121 gaatgtacgc agctacatta atttcgccgt tcgaatgcca gtgctttgcg ctcgatcaga
      181 cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccctgc cgcaccgaac
      241 accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac ttccatcagc
      301 gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt ggaataagaa
      361 gagagcgagc gtttaaaggc atacggcagc aggatcgcca gcgtatcttt tttgctcatt
      421 cccagggcgc tacaggactg ccactgacct tctgggatcg cacgaattgc accgtaaaac
      481 agctgcgtgg tatacgccgc actgttcagc gataacgcaa tcagcgcaca taaccacggt
      541 tctgacaaca aatgccacag tgccggatac tcctgcaaag tcggaaactg gcccggcccg
      601 taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt gatataaccc
      661 cgcaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc
      721 agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg tagccctttc
      781 atcagttcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca
      841 ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa atcgccgccg
      901 ccacaatgta ccaggtaaat ggttcctggg tacgagtagc gatgcttttt gtttgcagca
      961 ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc accagccact
     1021 ggttaccgag gccaggcagc gcatgacgcc acatctgcgg catcaccaga cggaaaaaga
     1081 tagccgattt cgacagcccc agcgcctgac cagactccca ctgacccacc ggcaccgctt
     1141 tcaacgcgcc ccgaagcgtt tgcgaggcat aggcggcata cagcagtgac agagcgatga
     1201 caccacaaag gaacgggcta acgtcgaagt tctcaatgtc catctgcact gggatctgca
     1261 cgaacccaag attgatagtg aagccatccg aaagcgtcag cagcagctgc gaggagccaa
     1321 aatagataaa cagcaccacc agaatttctg gcaggccacg cagaatggtt accagcgctg
     1381 aacctgccca cgcgacagga cgccattttg ccgactccca taccgcaaag aacatcgcca
     1441 gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg
     1501 ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg tagatggttt
     1561 cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gagtttctgc tgcagctcag
     1621 tgttgccctg acgtaccgcg atgccgaggc cagtgccgaa gtaatcttta tcggtcactt
     1681 tgtcgcccac cgccgccagt ttcgggttat ctttcagcca ctcagtgacc actgcggtgt
     1741 caccgaagac gccgtcgata cgcccgtttt gcagatccag ttttgcgttc tggtagctgt
     1801 catacggaac ggtagtgatt tccgggtgct tatccataat gaatttctgg tgtgtcgtcc
     1861 cgttctgaac gccgactttt ttgcctttca gctgatcaac actggtgtat ttgccttgct
     1921 gacccacaaa cagggcagag ttgtcatagt acggggtggt aaacagtacc tgcttttcac
     1981 gctccggagt gatatccatg cccgccatca ctgcttctac gcgacggaat ttcaggcttg
     2041 ggatcaggct gtcaaacgcc tggttagaga aagtacaggt tgcatcaatc tctttacaca
     2101 gtgcctgtgc caggtcgacg tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa
     2161 acggaggata ggaggcttcg gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac
     2221 taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc ccgaatctta
     2281 gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc
     2341 ttgttctacg atatgaccat tttccatata caccactcgg ctggcggttt tacgcgccac
     2401 ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct cacgaatgat
     2461 gctgacgatt tgtgccgtaa tttccgggtc cagtgcggcg gtcggttcat cgaacagcag
     2521 tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct gaccaccaga
     2581 aagatgcagc gggtaacgat cgctataagg tttgagacgc agacgttcca gcagtttttc
     2641 tgcacgggcc agcgcctgat ctttactcaa ccccagtaca cggcagggcg cttcaatcag
     2701 gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca tgccaacgtt
     2761 acgacgcaaa tcgcgaatcg ctttgtcaga gggtgttttg gtgaaatcga aatggttgcc
     2821 tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac gcagcagcga
     2881 gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag
     2941 cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat
     3001 actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga tagtaccttt
     3061 gacagaataa ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc attattcaat
     3121 aaacatagca caaaataacg ggggcggtgg tcggtgagca taaatgtcgg cattcctcac
     3181 gaaatgccgg acaatttacg gggtttattg gttgatcaag gcgttagcga ttctcgatgg
     3241 actgacggag cgtacccgct gtggcatgaa cgctgccgcc taaatagcgt acatcgtcga
     3301 tcacccaaca ctggccttcc tgaatcatta acacttcatc ctgccagctc tgatcaccct
     3361 gttttagatc cacacgcaga ggaatatttc gcgcatcacg attcgggatt gttgaggcac
     3421 tggcaacacg ggcgctctct ggcaaggttg tgcgactgga gaatggatcg ttggtcagta
     3481 gttcccgatg gttattatcc cgggaggcat cgctaagcag tgtcgccagt ttgtcgctca
     3541 gataagggcg caaggcggtg atgtcgttgc tgcggtgcaa aatgcggtag tcataaaatt
     3601 gctgggccac gttatccggg cctccttcaa cgcaaggacc actgcgtgtg ccgttatctt
     3661 tataagctgg agtgactgtg gtgcaggcac tgaggagcag tgcgcagggg ataagcattg
     3721 tcaatttgct gtagcgcata atgatttcct tataagcgat cgctctgaaa gcgttctacg
     3781 ataataatga tatcctttca ataatagcgt atcagtctga taatgcttta gagatcgaag
     3841 gcttaacaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct ggaaggccag
     3901 accatcgttg aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc caatattttc
     3961 cgtgatttct ttgccggtat ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa
     4021 gaactgcgta aagcacggga gatcgccttt gaggaattag gctcccaggc gcgggcgctg
     4081 ggggccgatg ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca aaacggcagt
     4141 atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct
     4201 ggctgatcgc tgccgctctg ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga
     4261 aagagggata tcagattgat acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag
     4321 tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca ctgaccgata
     4381 agcaggtcag ctcgcattat ctggtccctg cggtaccacc gcgatacaac ggtaaaccgc
     4441 gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc
     4501 gcggggcaac gcgccttaac gacacctcta ttggcattga gctggaaaac cgtggctggc
     4561 aaaaatcggc cggagtgaaa tattttgccc cgtttgaacc ggcacagatt caggcgctta
     4621 ttccactggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtggcac
     4681 atgcggatat cgcaccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac
     4741 tggcgcagca ggggattggt gcctggccgg atgcgcagcg ggttaacttt taccttgccg
     4801 ggcgcgcgcc gcacacaccg gtagataccg cgtcattgct ggagcttttg gcgcgctacg
     4861 gttatgacgt taaacctgat atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc
     4921 agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg
     4981 ccgaagcatt gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgta
     5041 gccaggcggc agttttctcg ataccttcat ccagggtgat gaccggctga taacctaact
     5101 cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag acgccgtagt
     5161 gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg cgggcgatca
     5221 tatccagcat cgggtagggg acggaacgaa tacgacagtc aatattcaac tcgtcgatca
     5281 gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc
     5341 cggaaggtag cttatcgcag gcttcctggc ttgccagcca cattgcgtgc acggcatttt
     5401 catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata ctgccgtagt
     5461 ggtgcatcat atgcgccaga cggggaataa agactttatc gtgcggtccg aacagacttt
     5521 gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt
     5581 cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt
     5641 ctttaatatc gcgatggtgg tgataatcga agtacaggga gggggaagag atatgaataa
     5701 agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg cgaacgttag
     5761 ccagatcgaa agcctgttgt gtcccccagg gtgaggtaaa gctggagcag tgccacagcg
     5821 tatcaatgcc cgcgagcatc actttagctt gtgatgaaac cagctcggtc agatccgccg
     5881 gaacaaactc tgcgcccatt ttttccagca atttgcccat tgcctcgttg cgaccggtcg
     5941 ctcgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg
     6001 tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat aacgtgcgca
     6061 ttcttccgtg atttccccca tgatgcaatg ggaaacatga aagaataacg caggttttgt
     6121 cgattaatct gtgctttgtt ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat
     6181 aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg
     6241 ccagaaagcg cggacatcga tagtacgata gtcgccttta tcttccaggc taaaacacag
     6301 tcgtcccagc cccggcgctt tcatgccaaa taacaacgta agttgttttt ccggttcaac
     6361 gacaatcact ttccagctat ctaccgcatc gccagtctgt aaatattcgc gctccgggcg
     6421 gcctttcgcc agcttatgac cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat
     6481 attgccaaag aaataacgct ctttaccgcc gatttggttc actacctgcc ataaagcagc
     6541 aaggctggcg gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac cgtactccgg
     6601 tcgccagcgg gcaaaggcct gagcgtcgta gccccagtcg ctggagttga ccagtttttc
     6661 ctcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag
     6721 tgcacgtagc gcggtatcat ccgccagcag atcgtgtttc agcccctgaa tcaacgccct
     6781 ggcggtggtg ggcggtacgg aagtaatcac attgagaaac cacaccgaaa tccagcgggt
     6841 ggggaggggg atggggatca accagcggcg cttaccgctc accgccataa aatgttcaaa
     6901 ctgttgctga taactgagca cctctggtcc ggcggcttcg aagatgcggt gttcgctggc
     6961 cggatgatct aacaacgcca ccagatagtg cagcaagttt tccagcgcga tgggcgtggt
     7021 gcgtgaacgt acccagcgtg gcggcgttaa cactggcagg ttgtagacca tatcgcgcat
     7081 gacttcgaac gccgctgaac ctgcgccaac gataattccg gcccgaagtt cggtcacagg
     7141 tacattcgct tcacgaagaa tgtccgccgt agcctgacga gcacgcagat gatccgactg
     7201 ctcatgtggc ggggcctgca acgaactgag aaagattaat tgcttaactg gtacttcacg
     7261 tagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat cgccgccttc
     7321 gcccatgctg tgcaccagaa aatagacggt atcgatatcc tgcaacaggg ccggaaggtt
     7381 atccggccag ctgagatcga ctttatggca actgacgttt gccagttgca gctttgcaag
     7441 cctgtcgaca tgacgtgccg ccgccaggat ctgatgccct tgctggctga gtgtgcgcac
     7501 cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg gcacgtctct
     7561 ctccttaacg cgccaggaat gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg
     7621 agacgtcaag atgcgtcacc aggcggacaa tcggcgaggc gttaatcagc acgtttctcg
     7681 ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca aacagcatat
     7741 tagtgtcctg acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg
     7801 cgttgtcgtg atcttcctgc aaccgtgcga cgttattttt cagggcatac atcccggcgg
     7861 cagccagaat cccggactga cgcatcccgc caccggccat tttccgccag cggatggcac
     7921 gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca agacctttcg
     7981 acaggcaaat ggtgaacgaa tcacaatatt gcgtgatctc tttcagttcg cagccgtaag
     8041 ccaccacggc attaaagatg cgcgcaccgt caacatgcag cgccagattg cgctcgcggg
     8101 taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg tgggtgtttt
     8161 ccagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca
     8221 ctttatccag cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc
     8281 ccagcaccgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga ccgacaatat
     8341 actcttcgcc acgttcgcag tgactgagca gagcgaccag gttggcctga gtgccggtag
     8401 gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc agagcattaa
     8461 cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatc gcttcgagca
     8521 tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca tgtccttatt
     8581 atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt tcgatcactt
     8641 catcgccgcg tccgctgata attgcgcgca acatatacag gctaaaacct ttggcctgtt
     8701 cgagtttgat ctgcggtgga atggctaact cttctttggc gaccaccaca tccaccaaca
     8761 ccggaccgtc gatagagaag gcgcgttgca gggcttcatc aacttcagac gctttttcta
     8821 cacggatacc cgtaatgccg cacgcttcgg caatgcgggc aaagtttgtg tcgtgtagtt
     8881 cggtgccgtc agtcaaatag ccaccagctt tcatctccat cgccacaaag cccagcacgc
     8941 tgttgttaaa gacgacaatt ttcactggca gtttcatctg cactactgag aggaaatcgc
     9001 ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctgacgt tctggctctg
     9061 tcgcctgcgc acccagcgcc tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac
     9121 ctaacaggcg acgcttgccg ttcattttta gataacgtgc cgcccacacc gttggcgtac
     9181 caacgtcaca ggtgaaaata gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat
     9241 attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct ttgcgggcgt
     9301 cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggctttttct tccaccaatg
     9361 gaagcaatgc acgcagagtc gacttgatat cgccgaccag tgccatatcc accttgctgt
     9421 gagcgccgat gctggctggg ttgatatcaa tctgaatgat tttggcatcg gtcgggtaga
     9481 aggcgcggta gggaaattgc gtgccgagta gcactaacgt gtcggcgttc atcatggtat
     9541 ggaaacctga cgagaagccg attaacccgg tcattccaac atcatacgga ttatcgtatt
     9601 cgacatgttc tttaccgcgc agggcatgaa caataggcgc tttaattttc ccggcaaact
     9661 caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg atattgctgg
     9721 aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcgtc acgactggtt
     9781 gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac gccacgtcgc
     9841 ctggtaacac gacaaccgaa acgccacggt taagcaccgc tttgcgcatg gcaatcgcca
     9901 gtacttgtgg gatctgctcc gggctggaaa ccaactcgca atagtgacta cattcgcgga
     9961 atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg gagggaatat
    10021 gagcggcaat cgccagtacc ggaacgtgat tgcggtggca atcgaacagg ccgttgatta
    10081 agtgcaggtt gccggggccg cacgatccgg cgcagaccgc cagttctccg ctaagttgtg
    10141 cttcagcgcc agcggcaaag gccgccactt cttcgtggcg ggtggacatc cactcgatgg
    10201 tgcccatgcg attaagactg tcactaagac cgttcagaga gtcgccagtg actccccaga
    10261 tgcgtttcac ccctgccgat tcgagtgttt tggcgatata agctgcaacc gtttgtttca
    10321 tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg acggaggggg
    10381 aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag gaatatcatc
    10441 gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag caggccagta
    10501 cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc gtatattcac
    10561 cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca gcgacaaccg
    10621 gaacgttatt gctttccagc gcctccagta gtgtggtgcc aaccggggcg taaaattctc
    10681 gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct actggggtga
    10741 agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat
    10801 ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca ggtacacctg
    10861 ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta ttttctgcca
    10921 ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc ggcgtacgca
    10981 cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag cgacgcatcg
    11041 acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct tctgctttat
    11101 cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg cgttttacat
    11161 cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc agggtgatat
    11221 attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc
    11281 gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtaatcgtgg caaatcaggg
    11341 aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc cgccacgggc
    11401 attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc
    11461 ttcttcaaca gtggtgatag aacgcaggcc aaatttctcg ttcagcaccg ccagcaggtc
    11521 tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc ccagagaaag
    11581 cagcgtcagc agaatgacga tcgctttctg ttcaaaccag gagagcacca gcgacagcgg
    11641 cagatcgttg acaccgcagc ccagtttctc tgccagagtg acagccagaa taatcgctga
    11701 gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga tatcgccaaa
    11761 ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc agtcatccgg
    11821 cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc
    11881 aagcaggaag atatgacgca gtttttcacg gctcaccaga tcaatcagcg tatcagcagc
    11941 gccaagcagc gtctggcgac caaaacccac ggtgatcagg tgcggaattt cgctgtacgg
    12001 gaagcccgcc atttgttgcg cctgggcgat gaccgcagaa aaatcttcgc catccagatg
    12061 acgcacaccc ggccagccaa cgatgctgcg ggtccagata cgatcgtcat aagcgcctac
    12121 ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac gagcgaactc
    12181 cacttgctga ttctgccagc cactgccgta gttaccgacc agatgcttga atttacgcag
    12241 ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc
    12301 ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa tcagaatgca
    12361 tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt atttaccggt
    12421 ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt ccattgaaca
    12481 ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga ttttatggta
    12541 ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca tataggccgc
    12601 cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt cgccaatcgc
    12661 cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc cgagatcgtc
    12721 gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat ctacagccag
    12781 gcattgcgct ttcagcgcct cgcgcagggc aatcgcttca cgagcgtagc cgacaatacg
    12841 cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc
    12901 aacatcgtgg ttgatgatgc cgtattcacg cgcttttacc gcccaggcag aaagcccttg
    12961 cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac cacacatccc
    13021 ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac attgcacaca
    13081 aaacatgatc acacctttta aagttatatt taatatacat gtttaaggtt aagacgctta
    13141 acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta gcgcaatttt
    13201 ggcggcaggg atctaccgcc agagaggtat tacgcagaga aaaaggcgat gaggatcggc
    13261 actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc cagcccgcca
    13321 gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa gcccagtgca
    13381 gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag ttcacgggcc
    13441 agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt caataaaata
    13501 ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt gatggggaga
    13561 tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac caccacggcg
    13621 acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt attgcgcaac
    13681 tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt
    13741 tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac gccgcacagt
    13801 tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg atggtggttg
    13861 cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag taaaataacg
    13921 gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag gttatcgaga
    13981 aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct taatagctga
    14041 ttaataactt ttaacgcagc ttgttggcga agcggaatga ggtaacccac aatcagggga
    14101 accagaatga ttaacagccc agaaaacatg aaaacccagt ccttgcaaag atgaagtcga
    14161 aatgcgcgat gacacactac tgaaagcgga aggacgagta aagttgcaat taaaaggaaa
    14221 tgttatgcat aaggagcagt agagtattcg ttttcattta aagatattct tgcgctttaa
    14281 ttacaaactg caccgatgtt ggtggcgtca aaatcgccga ggcgttccct gaaggccggg
    14341 gcagcccaca tggatgtggg ctgagggcgc gttttacagg gatgttacct cgcgcccgac
    14401 ccggtagccg taagggataa
//
LOCUS       DAZGCS010000118        14265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGCS010000118 DAZGCS010000000
VERSION     DAZGCS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.120"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..1755
                     /gene="garD"
                     /locus_tag="ACHK01_004021"
     CDS             184..1755
                     /gene="garD"
                     /locus_tag="ACHK01_004021"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO8098889.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGMAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1904..2239
                     /gene="prlF"
                     /locus_tag="ACHK01_004022"
     CDS             1904..2239
                     /gene="prlF"
                     /locus_tag="ACHK01_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO8098890.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2239..2703
                     /gene="yhaV"
                     /locus_tag="ACHK01_004023"
     CDS             2239..2703
                     /gene="yhaV"
                     /locus_tag="ACHK01_004023"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO8098891.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2758..3567)
                     /gene="agaR"
                     /locus_tag="ACHK01_004024"
     CDS             complement(2758..3567)
                     /gene="agaR"
                     /locus_tag="ACHK01_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO8098892.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3816..5096
                     /gene="kbaZ"
                     /locus_tag="ACHK01_004025"
     CDS             3816..5096
                     /gene="kbaZ"
                     /locus_tag="ACHK01_004025"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO8098893.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYTSANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARAMLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5119..5592
                     /gene="agaV"
                     /locus_tag="ACHK01_004026"
     CDS             5119..5592
                     /gene="agaV"
                     /locus_tag="ACHK01_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO8098894.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5603..6382
                     /gene="agaW"
                     /locus_tag="ACHK01_004027"
     CDS             5603..6382
                     /gene="agaW"
                     /locus_tag="ACHK01_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO8098895.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6372..7250
                     /gene="agaE"
                     /locus_tag="ACHK01_004028"
     CDS             6372..7250
                     /gene="agaE"
                     /locus_tag="ACHK01_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO8098896.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7268..7702
                     /gene="agaF"
                     /locus_tag="ACHK01_004029"
     CDS             7268..7702
                     /gene="agaF"
                     /locus_tag="ACHK01_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO8098897.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7699..8832
                     /gene="nagA"
                     /locus_tag="ACHK01_004030"
     CDS             7699..8832
                     /gene="nagA"
                     /locus_tag="ACHK01_004030"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8098898.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAQPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGNTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9183..10337
                     /locus_tag="ACHK01_004031"
     CDS             9183..10337
                     /locus_tag="ACHK01_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO8098899.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10350..11210
                     /gene="kbaY"
                     /locus_tag="ACHK01_004032"
     CDS             10350..11210
                     /gene="kbaY"
                     /locus_tag="ACHK01_004032"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO8098900.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11377..11853
                     /gene="agaB"
                     /locus_tag="ACHK01_004033"
     CDS             11377..11853
                     /gene="agaB"
                     /locus_tag="ACHK01_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO8098901.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11892..12695
                     /gene="agaC"
                     /locus_tag="ACHK01_004034"
     CDS             11892..12695
                     /gene="agaC"
                     /locus_tag="ACHK01_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO8098902.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAAIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12685..13476
                     /gene="agaD"
                     /locus_tag="ACHK01_004035"
     CDS             12685..13476
                     /gene="agaD"
                     /locus_tag="ACHK01_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO8098903.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13477..14232
                     /locus_tag="ACHK01_004036"
     CDS             13477..14232
                     /locus_tag="ACHK01_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO8098904.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQQLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3453 a         3608 c         3719 g         3485 t
ORIGIN      
        1 cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc cctgaaatga
       61 ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt
      121 catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg agaatatagt
      181 acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac
      241 gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt
      301 ccggatggac tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac
      361 attccggcta atggtgaaat tattcgttat ggtgaagtga ttggttacgc cgtgcgtgca
      421 atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta
      481 cacacgctgc cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc
      541 tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc
      601 accagcgtcc actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat
      661 ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt
      721 ggcatggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg
      781 ctgaacccta actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag
      841 cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc
      901 attgtcagtt tgcaggatga aaagcatgtc ggttttcagt ccatggtcga ggatattttg
      961 caggtcgccg aacgccatct acaaaaactg aatcaacgtc agcgagaaac ctgtccggct
     1021 tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg cgtaacggca
     1081 aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttc
     1141 tcagaagtca cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa
     1201 gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga
     1261 aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaacgtg
     1321 gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt tgaagtgctg
     1381 tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat
     1441 tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt
     1501 cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcgac ccgcaccgag
     1561 ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc caccggcgaa
     1621 gaaaccattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg
     1681 aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcagt gtttaacccg
     1741 gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt
     1801 ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc
     1861 ttataatttg agcagaaaac aggctgtaaa aggacagtga atcatgcccg ctaatgctcg
     1921 ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc
     1981 cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat
     2041 tctgcctggt gggcaagtct ttatgtgccg actgggagat gaacaggagg atcatactat
     2101 gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa aaactcgtcc
     2161 attcaacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca ttgatgatga
     2221 gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc gctatatgct
     2281 catccctgtt ttcaggaaac ctacgacgct ttagttgccg aagtcgagac attaaaggga
     2341 aaagatcctg aaaattatca gagaaaagcc gccacaaagt tattggcggt agtccataaa
     2401 gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg
     2461 ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg tcgttatcgt
     2521 ctcttctttc gttatagtga aaaagagaaa gtcatcattc tgggatggat gaacgatgaa
     2581 aacactctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag caaaatgtta
     2641 aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga agaaaaccat
     2701 tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga ccagcgggca ccctttctca
     2761 ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg
     2821 aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact
     2881 ggaacgattg aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat
     2941 ccgacggttt aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat
     3001 cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg
     3061 atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac
     3121 gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc
     3181 agtgtgctta cgcatcagac gagcaatctc aaaagtggta gtcccggaat cgaggatcac
     3241 ccgatgacca ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt
     3301 gttcagcgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat
     3361 caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt
     3421 gcggatcgtc acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc
     3481 ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgccag tcactcgctt
     3541 ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt
     3601 cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc ccattgacgc
     3661 aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga tcagaaaact
     3721 attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa caaaacgaaa
     3781 gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga
     3841 gacagcacaa agcgggcaaa acaaatggaa tttatgccgt ttgttccgca catccgctgg
     3901 tgctggaggc tgcaatccgc tacaccagtg caaaccaaac gccgttactg attgaagcaa
     3961 cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg
     4021 gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg attctgggtg
     4081 gtgaccatct ggggccaaac cgctggcaaa acctgccggc cgctcaggca atggccaatg
     4141 ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca
     4201 gcatgtcctg tcaggacgat ccgattccct taactgatga catcgtggct gaacgcgccg
     4261 cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa gccgatctgg
     4321 agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc
     4381 tggcggtcac cacgccggat gccgcccgcg ccatgctgga agcccatcgt cacgcctttg
     4441 aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt caacccggcg
     4501 tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc
     4561 agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc
     4621 cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccagcgc
     4681 tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa gaactggtgc
     4741 cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga cgtgatgctc gaccgcccgg
     4801 aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttata
     4861 gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc
     4921 atctggtacg taatctggcg gattcaccaa ttccgctgcc gctgatcagc cagtatctgc
     4981 cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg gaactcatta
     5041 tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa
     5101 aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt
     5161 cacggtcagg tcggcgttca atgggtcgga tttgcggggg caaatctggt gctggtagcc
     5221 aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa
     5281 gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc
     5341 gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa
     5401 ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa
     5461 caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa
     5521 gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt
     5581 aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat
     5641 tatcgccttt atcgctggcc tggatatgtt taacggccta acacatatgc accgcccggt
     5701 ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg
     5761 cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa
     5821 cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga
     5881 tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct
     5941 gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac
     6001 tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct
     6061 ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct
     6121 gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt
     6181 tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca tcctgggctt
     6241 tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgctg cgctggcgat
     6301 ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga
     6361 ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa aacgaagaaa
     6421 cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc gcggagctga
     6481 cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact
     6541 acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc
     6601 acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc
     6661 atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt tctaagcagg
     6721 acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc gggattggcg
     6781 atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gataggcgca agccttgcgc
     6841 tacaaggctc tattcttggc gcagtcgtat ttattgtgct gttcaacgtg gtacacctgg
     6901 ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg attccactca
     6961 ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg acggtaatcg
     7021 gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca gcgggcgacg
     7081 cagtggttaa gttacagact gatgttattg acaaactgat gcctgccttc ttaccgctgg
     7141 tctacacctt gaccatgttc tggctggtac gccgcggctg gagtccgctg cgcctgattg
     7201 cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa atacaatgag
     7261 gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa
     7321 agcaatgaag caaatcctcg gcgagcaatc gcagtttatt gccatcgatt ttcccgaaac
     7381 ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga
     7441 agacggcatt gtttttctga ctgatttgct gggcggcacg ccgtttcgcg tagcttcaac
     7501 gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttac aactgctact
     7561 ggagatggtg ctggagcgcg aagggttaag cggtgaagag ttccgtgtgc aggcgctgga
     7621 gtgtggacat cgtgggctga ccagtctggt agacgagtta ggccgctgcc atgaagaatg
     7681 tccggtcgag gaaggaatat gacacccgtt ctgcgcgcca gaaggctgct gactgaagag
     7741 ggatggctcg atgaccatca gttgcgtatt gctgacggtg tcatcgcagc aatcgaaccg
     7801 attccagtgg gcgtgactga acgcgatgcg gaactgctct gccctgctta catcgacacc
     7861 catgtacacg ggggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag
     7921 ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg
     7981 ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga
     8041 cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa
     8101 ggcgcgcaac cgccggaatt gtttcgcgag cttgaaattg ccgagctgga tcaattgatt
     8161 gccgtttctc agcacacctt acgcgtggta gcgctggcac cggaaaaaga gggggcattg
     8221 caggccatcc gccatcttaa acagcaaaat gtacgagtga tgctggggca tagcgcggcg
     8281 acctggcaac aaactcgcgc cgcgtttgat gctggtgccg acggcctggt gcattgctat
     8341 aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac
     8401 aagcgcgcct ggctggaact gatagccgat ggtcatcatg tgcatccggc ggcaatgtcg
     8461 ctgtgttgtt gctgtgcgaa agagagaatc gtactgatca ccgacgcgat gcaggcagct
     8521 gggatgccgg atggtcgcta tacgttatgt ggtgaagaag tgcagatgca cggtggcgtt
     8581 gtccgtaccg cgtctggtgg gctggcgggc aatacgctgt ctgttgatgc ggcagtgcgc
     8641 aatatggtcg agttgacggg cgtaacgcct gcggaagcca tccatatggc gtcgctgcat
     8701 ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg caaacgcgcc
     8761 agcgtcgttg cgctggatag cgggctacat gtgcaacaaa tctggattca gggtcaatta
     8821 gcttcgtttt gatggtttgc tccattattg ggccttcact tcccccgtaa ggcctttctt
     8881 tttctttctt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt cttatgcgac
     8941 ctacggtacg ccgcatccgg caatcggtga acatagcgac gtcattacgc aattctttaa
     9001 tcttcccttg tcttttcttt caacgatcac agatttcgtt ttatttcctt tttcttcatt
     9061 gaactttctg tttcttttct atagatttta atcaacgaaa gacatcacca agtgaaatta
     9121 aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact aaggattgag
     9181 ttatgccaga aaattacacc cctgctgccg ccgcaaccgg tacatggact gaagaagaga
     9241 tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg ctacgttccg
     9301 cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc atcctgaccg
     9361 gagccggaac ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc agccataccg
     9421 gtaaaaactt cagcgccgta ccgaccaccg atctggtcac taatccgatg gactacctga
     9481 acccagccca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg
     9541 tcgctgccgt ggaactggca aatcaatttg taccagaatg ctatcacctg ccgatcacct
     9601 gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg tttgccctgc
     9661 tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt accaccatga
     9721 tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg
     9781 tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa ggtgtgtttg
     9841 gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag ggcgcagcac
     9901 gcgagtcggc gctgaaagtg ctggaactga cggcgggtaa actggcggcc ttttatgatt
     9961 ctccaaccgg attccgtcat ggaccaaaat cgctggtcga taacgaaaca ctggtggtgg
    10021 tatttgtctc cagccaccct tacacccgtc agtatgatct tgatctgctg gctgaactcc
    10081 gccgtgacaa ccaggcaatg cgcgtaatcg ccatcgccgc ggaaagcacc gacatcgtcg
    10141 ctgccggtcc acatattatc ctgccgccgt cacgtcactt tatcgacgtt gagcaggcat
    10201 tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac atgggcaata
    10261 cgccggatac cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc
    10321 atccgtggca ggcataagag gatcgcatta tgagcattat ctccactaaa tatctgttac
    10381 aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac gccgagacga
    10441 tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc gccggaacgc
    10501 cggggacctt taaacatatc gcgctggaag agatctacgc cctgtgcagc gcctattcca
    10561 caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg gatgatattc
    10621 gccgtaaagt ccacgcaggt gtgcgcagtg caatgatcga cggcagccac ttcccgtttg
    10681 ccgagaacgt gaagctggtg aaatcggttg ttgacttctg ccactcacag gattgcagcg
    10741 tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa
    10801 gtgcattcct gaccgatcca caagaagcta aacgctttgt cgaactgact ggcgtcgaca
    10861 gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg aagattgatt
    10921 tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgcta catggtgcca
    10981 gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg
    11041 ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt gcggaaaatc
    11101 cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg aaagaagttg
    11161 tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa tcattagttt
    11221 tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta atggaaaagg
    11281 agatgactaa accttaataa atacatcagt acaatatcgc aacaataata tattaaaaaa
    11341 aatcatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa tattctctta
    11401 acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac atccaccatc
    11461 ggtgcgaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat tcaacagaaa
    11521 ttaatgggta ttaccgcaga aacctacggc tttggcattc gtttctttac tatcgaaaaa
    11581 accattaacg tcatcggcaa agctgcaccg catcagaaga tcttcctgat ttgccgtacg
    11641 ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc tgaaagatat caacgtcggc
    11701 aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt cgatgaccag
    11761 gatctcgcgg acttacgttt tattaaacaa cgtggcgtga atgttttcat tcaggacgtc
    11821 cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa tattggtttg
    11881 aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg gcgttagttt
    11941 tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg ataatcgttt
    12001 gtaccctaac tggcgctatt ctcggtgata ttcagactgg cttaattacc ggtggtctga
    12061 cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc aacccgatta
    12121 tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc aaaacagcaa
    12181 ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc ttctattccg
    12241 ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat acggcagcgt
    12301 tttcccggct taactggaca acgatgctca tcgtcgcttc agcgtatgcg gcgattgctt
    12361 tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg atgcccgcct
    12421 ggttgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt tttggcttgc
    12481 tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct gatcgctggc ttcctgtttg
    12541 tttgctacat ccaggtcagc aacctgttgc cggttgccgt actgggtgca ggctttgcgg
    12601 tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc gttgccagta
    12661 aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga tatcacccgt
    12721 ctgggctttc gttcgtcgct gctgcaagcg agctttaact acgaaaggat gcaggcgggc
    12781 ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga caaaccgggc
    12841 ttaagcgcgg cgatgaaaga taacctcgaa tttattaata cccacccgaa tctggtcgga
    12901 ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga cactattaaa
    12961 ggcctcaaag tggcactgtt tggcccaatt gccgggattg gcgatgcgat tttctggttt
    13021 actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg aaacctgctg
    13081 gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt cggctggacc
    13141 cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa ctcgcagatg
    13201 attgctcgct cggcaaccat cctcgggatc acggtaatcg gcgggctgat cgcttcgtat
    13261 gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt cgcgctgcaa
    13321 caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac cctgctgatg
    13381 tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt gacttttgtg
    13441 ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac tgcgtctggc
    13501 ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt tgaaaactat
    13561 acggcgttaa gtgaacgtgc cagcgaatat ttattggccg tgatccgtag caaaccggat
    13621 gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta tctggtagaa
    13681 aaaatccacc agcagcaggt tgatgtcagc cagctcacct tcgtgaagct cgacgaatgg
    13741 gtggatctgc cattaacgat gcctggtacc tgcgaaactt tcctgcaaca gcatatcgtg
    13801 cagcagctgg ggctacgtga agaccagctc atcagctttc gctccgaaga gataaatgag
    13861 acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga tttatgcgtt
    13921 ctaggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag cctacaaccg
    13981 gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt aaaaaccacg
    14041 ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa tgcccgcgag
    14101 gttttgttat tggtgactgg cgaaggaaag caggatgcga cagaacgttt tctcacggct
    14161 aaagtctcca ccgctatccc ggcttcgttt ttatggctgc acagtaactt tatttgttta
    14221 attaatacct aaccagatga aaatttgctc aaaattttta aatta
//
LOCUS       DAZGCS010000119        13708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGCS010000119 DAZGCS010000000
VERSION     DAZGCS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.121"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..921
                     /gene="hlyE"
                     /locus_tag="ACHK01_004037"
                     /pseudo
     CDS             11..921
                     /gene="hlyE"
                     /locus_tag="ACHK01_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1128..1574)
                     /gene="ycgN"
                     /locus_tag="ACHK01_004038"
     CDS             complement(1128..1574)
                     /gene="ycgN"
                     /locus_tag="ACHK01_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO8098905.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1666..2325)
                     /gene="ycgM"
                     /locus_tag="ACHK01_004039"
     CDS             complement(1666..2325)
                     /gene="ycgM"
                     /locus_tag="ACHK01_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8098906.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2397..2690)
                     /locus_tag="ACHK01_004040"
     CDS             complement(2397..2690)
                     /locus_tag="ACHK01_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO8098907.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2932..3333
                     /gene="pliG"
                     /locus_tag="ACHK01_004041"
     CDS             2932..3333
                     /gene="pliG"
                     /locus_tag="ACHK01_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO8098908.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3453..3821)
                     /gene="ycgJ"
                     /locus_tag="ACHK01_004042"
     CDS             complement(3453..3821)
                     /gene="ycgJ"
                     /locus_tag="ACHK01_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO8098909.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4341..5036
                     /gene="minC"
                     /locus_tag="ACHK01_004043"
     CDS             4341..5036
                     /gene="minC"
                     /locus_tag="ACHK01_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO8098910.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5060..5872
                     /gene="minD"
                     /locus_tag="ACHK01_004044"
     CDS             5060..5872
                     /gene="minD"
                     /locus_tag="ACHK01_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO8098911.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5876..6142
                     /gene="minE"
                     /locus_tag="ACHK01_004045"
     CDS             5876..6142
                     /gene="minE"
                     /locus_tag="ACHK01_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO8098912.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6514..6858)
                     /locus_tag="ACHK01_004046"
                     /pseudo
     CDS             complement(6514..6858)
                     /locus_tag="ACHK01_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6893..7012)
                     /locus_tag="ACHK01_004047"
     CDS             complement(6893..7012)
                     /locus_tag="ACHK01_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8098913.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6973..7158)
                     /gene="ymgJ"
                     /locus_tag="ACHK01_004048"
     CDS             complement(6973..7158)
                     /gene="ymgJ"
                     /locus_tag="ACHK01_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO8098914.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7259..7432
                     /gene="ymgI"
                     /locus_tag="ACHK01_004049"
     CDS             7259..7432
                     /gene="ymgI"
                     /locus_tag="ACHK01_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO8098915.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7434..7778
                     /gene="ymgG"
                     /locus_tag="ACHK01_004050"
     CDS             7434..7778
                     /gene="ymgG"
                     /locus_tag="ACHK01_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO8098916.1"
                     /translation="MKKKILTFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7782..8117
                     /gene="ymgD"
                     /locus_tag="ACHK01_004051"
     CDS             7782..8117
                     /gene="ymgD"
                     /locus_tag="ACHK01_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO8098917.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPDLSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8174..10495)
                     /locus_tag="ACHK01_004052"
     CDS             complement(8174..10495)
                     /locus_tag="ACHK01_004052"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8098918.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10612..10821)
                     /locus_tag="ACHK01_004053"
     CDS             complement(10612..10821)
                     /locus_tag="ACHK01_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8098919.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11221..11439)
                     /gene="ymgF"
                     /locus_tag="ACHK01_004054"
     CDS             complement(11221..11439)
                     /gene="ymgF"
                     /locus_tag="ACHK01_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO8098920.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(11571..13094)
                     /gene="pdeG"
                     /locus_tag="ACHK01_004055"
     CDS             complement(11571..13094)
                     /gene="pdeG"
                     /locus_tag="ACHK01_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8098921.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13426..13674)
                     /gene="ymgC"
                     /locus_tag="ACHK01_004056"
     CDS             complement(13426..13674)
                     /gene="ymgC"
                     /locus_tag="ACHK01_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO8098922.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3914 a         3184 c         2958 g         3652 t
ORIGIN      
        1 gcgaatgatt atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat
       61 cgaaactgca gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg
      121 gcagaccttt gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc
      181 agcctccgtt ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt
      241 tgaagcaacc caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta
      301 tatttttcta tttgatgagt acaatgagaa gaaagcatcc gcccagaaag acattctcat
      361 taaggtactg gatgacggca tcacgaagct gaatgaagcg caaaaatccc tgctggtaag
      421 ctcacaaagt ttcaacaacg cttccgggaa actgctggcg ttagatagcc agttaaccaa
      481 tgatttttca gaaaaaagca gcttttccag tcacaggtag ataaaatcag gaaggaagcg
      541 tatgccggtg ccgcagccgg tgtcgtcgcc ggtccatttg gattaatcat ttcctattct
      601 attgctgcgg gcgtagttga agggaaactg attccagaat tgaagaacaa gttaaaatct
      661 gtgcagaatt tctttaccac cctgtctaac acggttaaac aagcgaataa agatatcgat
      721 gccgccaaat tgaaattaac caccgaaata gccgccatcg gggagataaa aacggaaact
      781 gaaacaacca gattctatgt tgattatgat gatttaatgc tttctttgct aaaagaagcg
      841 gcaaaaaaaa tgattaacac ctgtaatgag tatcagaaaa gacacggtaa aaagacactc
      901 tttgaggtac ctgaagtctg ataagcgatt attctctcca tgtactcaag gtataaggtt
      961 tatcacattt aaaaacacag acgtttttta aagattgaga gttacctgtt gataataagg
     1021 tatcgggtaa ctctcattat gacagagcgc tttcaaaaac agggctacga aatggcgagc
     1081 atccagacgt atattaagaa agcccccatc aacatctatt aaatttatca ctgcgcccag
     1141 tcaggtttat ttaagatatg atcctgccag tcaatcactt ctgattcttt cactgcgata
     1201 tgacgcacag agatacgttc accatgcatt gccgctttcg aaccagtaag tagcggatgc
     1261 cacgcaggta aatctttacc ttccgccagc aaacgataag cgcaggtcat tggcagccat
     1321 tcgaatgttg gcagattttc acgggttaat ttaatgcagt cgggttcaaa ctcgaaacga
     1381 cgttcgtagt tccgacattg acaagtttta atattgagct ggcgacaggc gacgttagtg
     1441 aagtagattt cgtcggtgtc ttcatccatc agtttatgca ggcaacactg accgcaacca
     1501 tcacacaacg actcccattc cgcatcgctc atttcgtcca gggttttact ttgccagaaa
     1561 ggtacatcgc tcatcaggtg ttccgccatt acgttaaaac gcaccttata accagtctgg
     1621 cacagcgatg caagttttgc cgccgctttc aggcggcaaa aagtattaca aaacgcgagt
     1681 tgtcaaagaa tgcccatcga aagtgactgt cagctcatca ccgctttgca acgggccgac
     1741 gccatcaggc gtgcctgtca acacaacgtc accggccttg agggtaaaaa acttgctcat
     1801 ataagcgatc aacggaacga ttttatggat catgtccgca gtcgtacctt gctggcgttg
     1861 ttcgccgttt acgctcaggc ccagcgttgt attttgtgga tcgccggtaa attccgccgc
     1921 ggggataaac ccggaaagcg gacaagagtt atcaaacgct ttagcctttt cccacggctg
     1981 cccggctttc ttcatttttc cctgaacatc acgcaatgtc agatcaagcg ccacgccgta
     2041 accggcaatg gctttgcgga catgctcttc cgttgcctga cgcagcgtcg cgccaatcaa
     2101 caccgccagt tcgacttcat gatgaactga accgaaatcg gatgggatcg ccaatggctg
     2161 ccgcagatcg cacagtgccg tttctggttt aataaacagc actggctctt cgggcactgc
     2221 gctgcccatc tctttaatat gtttggcata gttactgcca acacagacta ctttactcac
     2281 cggataatcc agcagcgcac cttgccagtt gtgatgttga tacataacgt tcccctcacc
     2341 ttatcgtgga tatgaaaacc cgcgccgaca aagataatgc caccgccgga tatcggttat
     2401 ttgttagtgt cgtctgtttt ctgccccatg acggaaagat gttgcttcag caaatcttcg
     2461 ggtggtggcg gtaactgcaa atagtaacct tgctcggtta atgccagttt aaccttttca
     2521 atatcagcat tgaccagttt cttgcgccca tccagcggca gaatcatcgc taactgaggc
     2581 tgaccaaaac ctttcatcag ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca
     2641 tataaatagg tctggtcacg cttgctgctt cgataaatca cacaaaacat acttttactc
     2701 tttaagatgc caggttttgg cataagctaa catcaatgaa acccgcgcca caagccagcc
     2761 agctgtagat ttctgcggtt tgttgagtcc atgccccttt ataagtaatc cccccggcaa
     2821 aaaatgccgc gccgggattt taccgaacgc agaaaagcca ctggaattct cccgtaaggg
     2881 attgttatat tataacagtt catcgtactc attctgaaca ggagactacc aatgaaaatc
     2941 aagagcatca gtaaggctgt attgctgctt gcactgctaa cctccacgag ctttgctgcg
     3001 ggtaagaatg tcaatgttga gttcagcaaa ggccatagca gcgctcagta ctccggcgaa
     3061 ataaagggat acgattacga tacatatacc ttctatgcca aaaaaggtca gaaagtgcat
     3121 gtgagtatct ctaatgaagg cgctgatact tacctcttcg ggccaggaat cgatgattca
     3181 gttgatttat ccagatattc accagaactc gatagtcacg gacaatactc actccctgct
     3241 tctggcaaat atgagctgcg agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact
     3301 aaaaaatata acgtcgatat tcaaattaag taaatgtcaa ccgacgggga ctctccccgt
     3361 cataaacgtt aacgccccac tcctctctgt cgctcaagag gttaatgacg aatagcagac
     3421 aatgccgaat agaacataat gtaaacaagg catcactcac gacacataaa taacatcttt
     3481 gtggtgattt gattgatttt tccactccgc ttaccatcca caccaaaata acgatcatca
     3541 cgacataatt tttctttaac atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga
     3601 tcgtagcctt gtaatgattg taagttttca gcggcttttt cgccgagata tttccttgtt
     3661 aactctgggg aaatgccatc tgagttcaca caaatatacg ggtcgcaaac aacgccgcac
     3721 gccggagatt gaagtccgcc agcgtcatgt ccagacgcca ttgatgagaa catcatcccc
     3781 acacctgaca atccgatata aaaaatacgc atcatattca tagcacatcc tgataatcca
     3841 accgtacaac acttatgctc atttcatcat caatccgtaa tcaaaaccgc tgacctggac
     3901 gagttacgat gacctgacag aagtatcaga cgtcttctgc ggtttctatg atagagcaat
     3961 tctgtggcgg atattaagat aacgggtatc tgttctttaa tgatgccgca aaaaatggaa
     4021 agttaagaaa gcctggcgtg gtgaaaaaat acacttgcct caatactgga tatttaaaca
     4081 ggtctggaga tgctgtcacg attagggtgc ggaaaaagga agcgtaaata agaacaatca
     4141 aaagtacgac ggcaatgggt tgattgacaa gggtattttt taagctatga atcagcgcca
     4201 tttatcacag aatagacttt tactctgaat aaatgggagg gtgacttgcc tcaatataat
     4261 ccagactata acatgcctta tagtcttcgg aacatcatcg cgcgctggcg atgattaata
     4321 gctaattgag taaggccagg atgtcaaaca cgccaatcga gcttaaaggc agtagcttca
     4381 ctttatctgt ggttcatctg catgaggcag aacctaaggt tatccatcag gcgctggaag
     4441 acaaaatcgc tcaggccccc gcatttttaa aacatgcccc cgttgtactc aacgtcagtg
     4501 cactggaaga cccggtaaac tggtcagcga tgcataaggc ggtttcggca accggtttgc
     4561 gggttattgg cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg
     4621 ggctgcctat cctgacggaa ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg
     4681 ctccagcgca aaatacaacg ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt
     4741 ccggtcagcg tatttatgct ccacaatgtg atctgattgt tacaagccac gttagcgctg
     4801 gggccgaatt gattgccgat gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc
     4861 ttgcaggggc aagtggtgac cgggaaacgc aaatattttg tacgaacctg atggcggaac
     4921 tggtgtccat cgcaggtgaa tactggctga gtgatcaaat cccagcagaa ttttatggca
     4981 aagcggcgcg actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc
     5041 tttttaacaa ggaatttcta tggcacgcat tattgttgtt acttcgggca aagggggtgt
     5101 tggtaagaca acctccagcg cggccatcgc cactggtttg gcccagaagg gaaagaaaac
     5161 tgtcgtgata gattttgata tcggcctgcg taatctcgac ctgattatgg gttgtgaacg
     5221 ccgggtcgtt tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt
     5281 aattaaagat aagcgtactg aaaatctcta tattcttccg gcatcgcaaa cacgcgataa
     5341 agatgccctc acccgtgaag gggtcgccaa agttcttgat gatctgaaag cgatggattt
     5401 tgaatttatc gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa tggcactcta
     5461 ttttgcagac gaagccatta ttaccaccaa cccggaagtc tcctcagtac gcgactctga
     5521 ccgtatttta ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat
     5581 taaagagcac ctgctgttaa cgcgctataa cccaggccgc gtaagcagag gtgacatgct
     5641 gagcatggaa gatgtgctgg agatcctgcg catcaaactc gtcggcgtga tcccagagga
     5701 tcaatcagta ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca ttaacgccga
     5761 tgcgggtaaa gcctacgcag ataccgtaga acgtctgttg ggagaagaac gtcctttccg
     5821 cttcattgaa gaagagaaga aaggcttcct caaacgcttg ttcggaggat aagttatggc
     5881 attactcgat ttctttctct cgcggaagaa aaacacagcc aacattgcaa aagaacggct
     5941 gcagattatt gttgctgaac gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt
     6001 gcgtaaagat attcttgagg tcatttgtaa atacgtacaa attgatcctg agatggtaac
     6061 cgtacagctt gagcaaaaag atggcgatat ttctattctt gagctgaacg tgaccttacc
     6121 ggaagcagaa gagctgaaat aagcccgctg taaaaacgca tttatcttca aggcagagtc
     6181 atttctgcct tgagtttttc atccctctca tccacgttgt ggtaaagcgg cgagtattct
     6241 tgctgatact cctcattgct atttccacct ccccctttac accttaaggc tgttcccacg
     6301 cacagaacaa aatgtttatg cctggttgag taaataaccc tgttgttagt gtggatatac
     6361 tgggagatga taaccccgca atttcattat tcattgttct cccataggat gaggtttccc
     6421 gccgtaactg gcgggctttt tttgccccag aattttctct ttcaacatcc tgtaaacgaa
     6481 aactgcgccg aagcgcagtt taatcgttga tggtcagaac gaccagttca caccagccac
     6541 cgcgttccac ggggactcca caccggcacc atggctatac cccaccccga aatgcccgct
     6601 taacgtactg ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa
     6661 atcattgaca aaattaccgt cactattcac tttcacccga ttatcatcga caaattcttt
     6721 gcgcacagcc gccttcaggc agggttcaat ttccataccg ttccccagac gcatgttgta
     6781 actcagcgtt gcacccagtt cacgatatat actgcgggta tcgactgatt tcgattccat
     6841 gccattggat aaatgatagt agcgtctaag tgacctaagc tctgctgcaa catcagcccg
     6901 caggcaccag accaggggat tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc
     6961 tcaccaaaga catcatgatg aaatgatgat atcccgcata agaaagaggc attttttaca
     7021 cgcagtgcgc tgaagtgtag ttggataaaa aatcactctt ttcagataat actgtcgtac
     7081 gcttatcttt cgacaatgag gccgtcggca aaataaaatg actaatgtaa tcgttgctga
     7141 tgaatatctt ctgctcacat aaaaatcaca caataatatt gataaatcag attatcttac
     7201 ctttacaaca ttgcgttaac agtcaatgac acttcttgaa taaaaatatt gtcaatcaat
     7261 gagttattcc tcatttaaga ttattctcat tcacgtaaaa aacataattc ctatcatcac
     7321 agccacgctc attttgaatt atctgaataa tagcgagcgt tctttagtta agcagatctt
     7381 aattgaagac gaaattattg tgtgtgcaac ttacttaata ccggacatct aagttgaaga
     7441 aaaaaatact taccttcggg cttatctcag cattgttctg ctccacgcca gcgatggcgg
     7501 atatgaatcg caccacgaaa ggtgcattgc taggcgcagg cgttggttta cttactggca
     7561 atggtgttaa tggcgtactg aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag
     7621 aaaaaggccg cgacggtaaa aatgcgcgta aaggtgcaaa agtaggtgcc gccgttggtg
     7681 ctgtcactgg cgttctgacc ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg
     7741 gcggtacggg tggtgcaatt ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg
     7801 ctttactggc gggcctgttt gttttcgcac caatgacctg ggcgcaggac tacaatatta
     7861 aaaacggttt gccgtcagaa acctatatta cttgcgcaga agccaacgaa atggcaaaaa
     7921 ctgacagcgc acaggttgca gaaattgttg cggtaatggg taatgccagc gttgccagcc
     7981 gtgatttaaa aattgagcaa tctccggatc tgagcgccaa agtggtagaa aaactgaatc
     8041 aggtttgcgc taaagacccg caaatgctgt tgatcacggc aattgatgac accatgcgtg
     8101 ctatcggcaa aaaataatat ttcatatgta ccgcgtcgat gaatacgacg cggtttttta
     8161 gctaacggcg ggatcagaac ttatatttca ggcccaccat cactgcggtg tcattgtagc
     8221 cattatcacc cagctgcacg cccacattcc cccagacgct ggccgctgga ttcaggttgc
     8281 catttacgcc aagtttaatt tcccccaaat tactcgcccc atcttgttcg actttcacac
     8341 cgttcattga gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt
     8401 caaagtcgtc accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaaac
     8461 caagtcgggt ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttcgg
     8521 tgtgcgcatt atgatcgacg cccatccaga tagcctgtgc ctgtggctgg aatatccacg
     8581 tgtatttcgc cgcttccaga ccaaaggatt tattcagggc aaagccgtaa cccgcttcca
     8641 gcgagacggt tgcgcctttc gcactatagg attcataggc taattcatcc cctttcaccg
     8701 agtttttaaa ccagctatat tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt
     8761 catcggcaaa ccaggtggca tacagccctg cgcaatagcc tctgacactc cctttcgaac
     8821 gataatccga cacgctggaa tgagttgagt tgtagtcgcg ggcataacca gccatcactc
     8881 ccaaacgcca actgtcactg tcggtaaaac caccggttaa cagatcggcc cctaactgcg
     8941 agacgtagcg atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc
     9001 cgccaatttg acgtagccaa agtcggcttg aacgctcctg ttcagttaca ggatcgatgt
     9061 agcgcatttc acccgcacgg tcgttcagac gcataacaaa aagagtgtta gcggctgcca
     9121 ggttggcgac gtaacttcca gcttcgaggc gcaggacggg ggtaggcgca acagtaggcg
     9181 ctgaacttcc tccggagttt gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc
     9241 agtttttatt ggtcgcttcg gtattacctc gggttaatga atattcgtag gcaccggcga
     9301 aaatcctcga atccttaata aattccccat ttgattcccc ctcaacgctg ataatctcta
     9361 tcccatttaa cgtgtaagct ccgctgcctc ctgcattggt aactttaact cgggttgttc
     9421 cagaggtatt tccattaata acgactttat ccgtagctga attatcatca cccaattcag
     9481 ttctgagatg caatacgcca ttattaccaa cgtaattttc agttattgtt aatcttgttg
     9541 gttcgacatc ccgtccatct gcccgggaaa tatagaccgt actattatca accgtgaggt
     9601 tgctcaccgt tgagtcagct ttaatatccc aggtactatt atctgaaagc gacagatagg
     9661 tatgagtgtt atcatctgtt gaaatattag ctgaacccgt tatttttgaa ttagttgcat
     9721 cgacggtcaa atgaccttta atattattga taaaaacatc tgcagccgat gatacctcac
     9781 tatccgtaat ggtcacgtta ctcgtctcat ttttatggta gaggatttca tttgctgacc
     9841 gtagtgagct accagataaa atatctaccg ttgcttcctg accatttatg gaagactcca
     9901 gcacaattcc agacccctga tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc
     9961 cattataaat tttagcagtc gcggcgtcag gacccgttgt ataaatttct ccctggttaa
    10021 atgaaactgt cccctggcgg gcagcgatgg catgagcatt gtccccatgc gtggtgatat
    10081 caacatcggt taatgtaacg ctggcattgg tataatcaag cgtgtcacca gccttgacga
    10141 cagtgccatt agcgtacgcg gcataacttt ctgttccgtg ggttgtgatc tccccttttg
    10201 ccatatccag ttttgctatg gcattatttt cattacttcc tgtatcggta gtactttctt
    10261 catcaagaga agatacacac gcacgcgcgc ttccgggaga tgcacacgca agaacgccga
    10321 ctgcgtgatt acccgcagta acaataaatg tatcatctcc gtcagaaaaa gagggggtaa
    10381 ccgaaccgtc agagttgact cttattatgg tgtcagtatt caggtttcca ctcgtttgac
    10441 ttagcaaacc aattgagtaa tcccccgtta tgacaatcgt gtttttaccc tgcaaaataa
    10501 cagcaccgcc aaattcactt acgattgcgc tagctgttaa tgctgtgctt agaggactat
    10561 tgtcagaagg atataaaccg cccgttgtta caatgacgcc attgttaatg gttaacttgc
    10621 taccctctcc tgcaacggat acccgtaact tccattaccc gttgttataa ctagcgcatt
    10681 atcaatatct tttacttttc catcttttat tgccacagca tgtttatatt catcgtgctg
    10741 aataaactgt tcagcatatg cattacccac cgccagagca ataagtccca aactaaaccc
    10801 ggagagtttt ttcagtttca tagattaacc atccattcat tggattaaca aacattcctg
    10861 gtcaaataac ctatgcgttc agattaccaa aacggagggt aaagacaaaa acaaaagcac
    10921 aaaaaattta aatagataaa cagcgacaat atataaatga taaataagtc ggcattatca
    10981 tttttcacct tattttcatg acattgatca ctttagggtg acgattttgt ttaacaaata
    11041 actcttcgac atcaaaattg ttaatagttg cgatgaggat tatttcggtg atatataaaa
    11101 acaaccgtga taacaccagg gttgacaact atttgcgcaa taatgccttt ccttcaatga
    11161 tccaggcgtt cactgccagc aacccattca gccgcatact gacagtgaga aactgaaaga
    11221 ttattgctga tccctgtggt tgacgagtct ttctcttaat cgattaacca gattgatcgc
    11281 cagccaaaca agggcattaa gcaaatttat cgtcacaagc acttctccca gcccggtact
    11341 gggttttgca ataaggatga ccaggagggt gatcagcata aatgcaatgg aaaagatgat
    11401 atagagagtg aaataatcca gattattact attgttcatg ctactaatgg caatcctgtc
    11461 aaaactttca aacaattata gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag
    11521 ctaattagct gtctataatt acaccgcact atggccttac tgatcaattt tcactcaacc
    11581 acaaccttca ccttcggttt agaaaggata attttgacca ggtcgatgta tgtaacaggt
    11641 ttatagaaat aataaccctg ctgaaatgtg atattatttt ggttcagata gtcaagttgt
    11701 tctttcgttt cgacaccttc agcgacgata ctgatcgaaa gtttacgcgc cagatccaat
    11761 acacaatcca gaattcgggt tgattctggg tctgcgttta cgcggccaac gaaactgtga
    11821 tcaattttga tataatcaat atgcaggtca tgaagataag aaagccctga gtagccagta
    11881 ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc
    11941 agactttcat caacattcaa tggctcacgc tcggtgactt ccagaacaag gtttaaatcg
    12001 cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga caaacgtcgg cgaaataata
    12061 tgcgaagcgc taaaattaat tccaatatga aaaccttccg gcaatttact cgcgatagcg
    12121 ttcatctgtc tggcaacctg attcatcagg ctttgcgtaa gcggaacgat taatccggat
    12181 ttttcagcaa gtggaataaa tgccgcgggt gatatatatc caccgtgagg ttgtttccag
    12241 cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac catttaccac aggttggtaa
    12301 aaagggacga tctccccatt atttattgcc cgtcgcagga tctcttcagg cgttgcgctt
    12361 ttattgaaat atctatcaag caggaaggca gcggcgcagg cgattaacag aataaatatt
    12421 aaaattccaa agcctttatc gataagtctg tagaagctaa agagtggcgg cgggttaaag
    12481 ataatagtga aatgatattt tgtggagtga accttctcgg tatagggata atgtccatta
    12541 agcgtcatca catcacccgt tggtccaata atgtcatccg ccacgcgtaa tacatacctt
    12601 acccctttca agggtacatt aagcgccccg cgaatatgtt gatcacttat cgtaaccaaa
    12661 atgcgcgtat ctgcaaattg gttctgatag agcaggatag gtaatctatt aacggtgtcg
    12721 attgctggag ccaacagtaa attactgtcc gggaaaacag gaatacgaga caacaggacc
    12781 cgattcccag gcagggatgt acaccagact attccctgtt tgagaatgat gattgtgcgc
    12841 aggtgaggtt tcagagctgc ttcagtgcca agctgatatt gctcctcgag gttgcattcc
    12901 ttcccggcaa tgttaaccgc tgtttgagtc gcttgtgacg cttcttcaag tatatgattg
    12961 atattgttgg cggcatatct cgctcccgcc aggtactctg cttttgcgga ataccagagc
    13021 tggatgttca aaatagcgac accggtaata aacaagcata tcgcaacgag gatgggtatg
    13081 agtgtattgc gcatttgctt gtcccggcca aagggtttaa tacatgaaaa tgccattcat
    13141 tcgctcgcgc tggcatctac agagagcatg gtagagagca taagaaatat ccacgccgaa
    13201 gataaaattt tctccatctt gatctaaccc attaattatt gagcataatg taggcagtac
    13261 aaaataagtt aggcgggata tcaggcgtca agaatggaga gcgagaactc tccattcttg
    13321 acacctgata ttgcggacat aataagaaag cataacgcct gaaatgctca ctttgcatca
    13381 gcatggtgat acagctgatg tttattctaa aaccttactc aagttctaag agagcacgga
    13441 ttccctgtca ttaataatga tatccgttag tgtagtctta tgcatttcaa tgtactgcaa
    13501 tatttgcaga tagagatcag aaagaatcac actggtttca tcttttaaac gcaagcgtaa
    13561 agcctgatca ataatatgac tatcaacata cccatgcgtc atgaacacct ccctctcgag
    13621 atttttaaac aaagcacatt gaaaaataaa tctctctggg attgaattat tcatgtcagc
    13681 ctcgtagaaa tattaaccca ataaaatt
//
LOCUS       DAZGCS010000120        13632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGCS010000120 DAZGCS010000000
VERSION     DAZGCS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.122"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            232..1062
                     /locus_tag="ACHK01_004057"
     CDS             232..1062
                     /locus_tag="ACHK01_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO8098923.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRKYERARLDKENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLSLFMKSPSYPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1203..1976)
                     /gene="uxuR"
                     /locus_tag="ACHK01_004058"
     CDS             complement(1203..1976)
                     /gene="uxuR"
                     /locus_tag="ACHK01_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO8098924.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2191..3651)
                     /locus_tag="ACHK01_004059"
     CDS             complement(2191..3651)
                     /locus_tag="ACHK01_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO8098925.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3732..4916)
                     /gene="uxuA"
                     /locus_tag="ACHK01_004060"
     CDS             complement(3732..4916)
                     /gene="uxuA"
                     /locus_tag="ACHK01_004060"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO8098926.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5256..6599
                     /gene="gntP"
                     /locus_tag="ACHK01_004061"
     CDS             5256..6599
                     /gene="gntP"
                     /locus_tag="ACHK01_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO8098927.1"
                     /translation="MHVLNILWVIFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTITNIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6842..7744)
                     /gene="fimH"
                     /locus_tag="ACHK01_004062"
     CDS             complement(6842..7744)
                     /gene="fimH"
                     /locus_tag="ACHK01_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8098928.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7764..8267)
                     /gene="fimG"
                     /locus_tag="ACHK01_004063"
     CDS             complement(7764..8267)
                     /gene="fimG"
                     /locus_tag="ACHK01_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO8098929.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8280..8810)
                     /gene="fimF"
                     /locus_tag="ACHK01_004064"
     CDS             complement(8280..8810)
                     /gene="fimF"
                     /locus_tag="ACHK01_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO8098930.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8820..11456)
                     /gene="fimD"
                     /locus_tag="ACHK01_004065"
     CDS             complement(8820..11456)
                     /gene="fimD"
                     /locus_tag="ACHK01_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO8098931.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMIQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11523..12248)
                     /gene="fimC"
                     /locus_tag="ACHK01_004066"
     CDS             complement(11523..12248)
                     /gene="fimC"
                     /locus_tag="ACHK01_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8098932.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12285..12824)
                     /locus_tag="ACHK01_004067"
     CDS             complement(12285..12824)
                     /locus_tag="ACHK01_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8098933.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLAYQ"
     gene            complement(12889..13437)
                     /gene="fimA"
                     /locus_tag="ACHK01_004068"
     CDS             complement(12889..13437)
                     /gene="fimA"
                     /locus_tag="ACHK01_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8098934.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3328 a         3506 c         3284 g         3514 t
ORIGIN      
        1 agcaacatca aattccaatt agtaataaga tatgcttttt aattgcatgc aaataatttt
       61 aaatgagcgt tttttgatac tttagagtgt ttcctaccct ttaatatcac catgtagggt
      121 tgatatcact acaacagaaa catgcatgtc atttgtttac ttctttttta ctacagaaaa
      181 atttatctat ttttctaaac atcagtgaaa tactcataaa aagtaactat tatgtctatg
      241 ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct tcatcatcca
      301 aaagtcgaca gtgagttaac acgtaaatat gaacgtgcac gcctggataa ggaaaatatc
      361 tatcttctgc ctcttgctcg tggcaataac cataattacg atggaaaaag cgttgtcgaa
      421 attagaaaac ttgatattag taaagagccc tggccattta attatgtgac agaaacatgc
      481 agagaatccg atggtattac aacaacagga cgtatgctct atcgaaactt gaaaatcacc
      541 tcagctttag acgaaatata tggtggtata tgtaaaaagg cgcatgctgc cacagagtta
      601 gcagaaggat tacggctaag tttatttatg aagagccctt cctatcctgt tgaggactat
      661 acagttcatg aaataacatt aggccctggt tgcaatgttc caggttacgc cggaaccacg
      721 ataggatata tttcaacact tccagtttct caggcaaaaa gatggaccaa tgagcaacca
      781 aggattgata tctatattga tcagataatg actgtctcag gggtggcaaa ctcttccgga
      841 ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc aataattggt
      901 atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa agttatcccg
      961 ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct tccagaactg
     1021 tttgaattaa aaaatggtga atggaattat attggtaaat agccactgag aagaatgagt
     1081 actaacattc aggttataca gatagctaaa caataccctg caatttacaa agtaatactc
     1141 attgcgactt cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct tattataata
     1201 agtcaggcgt cgactttatc cagcggccat gaatcaaaca gatagccatc aaaataaata
     1261 tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg ctgccacatt
     1321 gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg tttgtgatcg
     1381 cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg gatccacatt
     1441 ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat gctgttatgc
     1501 gttgcttcgg caatagcgag atggaactgc atgtcaccgc tttcgctgct gcccggcgca
     1561 ctggaagcca gttcacgctc ttccagttgc aatgcctgac gcattttgac gatatcttcg
     1621 cgggtagcct gcaaagcggc aaactcggcg atgttgctct ccaataactg ccgcgcctgt
     1681 aacagctcaa aaggaccggc atcgttgcag acgttggcat ccggactgtc tgtgttctgg
     1741 ctgcctgagt tatcaagaac atagataccg gcaccccggc gtacttccac cagccctttg
     1801 atctccagca tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag catttctgca
     1861 atttcacgct ccggcggcag ccgttcgcca ggattgtacg gcgtctttat gatcagatcg
     1921 cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga tttcatggtg
     1981 ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa cgccttatcc
     2041 ggcctacaac gggcaatttt acggcccccg ttaatctcaa actataccat tagcactgga
     2101 agttggcacg tataggtggg caaaaaaacg aaaactggtc aaccaaatat cagtttgatt
     2161 gaccagtgag tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc
     2221 gcgttcgcac agctgctgat atgccgccgt cactgcgcca acaaagtcgg cattctgcgg
     2281 cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt ctgcgccctg
     2341 atactgcgcg ttgaccttct ggaactccgc cagcatcggg tcgaccacgt caatggcatt
     2401 accctgctca tccacgccct gggtgtaacg catccagcca gccacgccca gcgccaggtg
     2461 acgccagctg ccgccgtttt gcaggtgcag acgcaccggg tccagcagac gctgcggtaa
     2521 cttctggctg ccgtccatcg caatctgcca ggtacggtga cgcagagacg ggttgctgaa
     2581 acgctcgatc agcagcgtcg cataggcgtt caggtccgta ccttccggca tcgacagcgt
     2641 tggcgcttgt tcctgcatca tcaaggagaa ggccgctttg cgataatccg ggttagtcat
     2701 ggtgtcggca atggtttcat agccgccgag gtaaccgagg tatgccagga aagagtggct
     2761 gccgttcagc atacgcagct tcatcatttc gaacggcaca acgtctgcaa cgaactgtgc
     2821 gcccacttta tcccagtccg ggcgaccgtt aacgaagtta tcttcaatca cccactgacg
     2881 gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg
     2941 caaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac
     3001 gttttcttca atccatgccg ccagctgcgg gtcacgcgcc tgagccagtc ccagtaccgc
     3061 gaccttcgcc acatggccgt tttcacgcac gttatcgcag gacatcaccg taaacgcttt
     3121 caaccctttt tcacgacgca gccgcagggc ttcgacgatg taaccaatcg cggacttcgg
     3181 cgcagtcggg ttttccagat cgtgcttgat cagtgggtta ttgagatcca gctgaccgct
     3241 tgccgcatca gcgcagtagc ctttttccgt gactgtcaga gagacaatcg ccgtctgcgg
     3301 acgcgccatc gcattgagaa taccttcgca gccgtcgatt tccggatgca gcgcttcttt
     3361 catcgaaccg ataattttca gctcggtgct ctctgcgcct ttttccgcta cggtgtacag
     3421 cagttgctgt tttttcaggt tttcgatcag cacgcggtcg ttgcctggca tcaggttaac
     3481 ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat acagcgcctg
     3541 gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaatg
     3601 atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca tcgtaaactc
     3661 cagaatccgt cttcaaagag tgacggcaag gaagaaggaa ccggcacggc gcgcagccat
     3721 gccggtggat attaacggct aaagaaagcg cgctggatcg ccagttcgac accgcgaact
     3781 tcggccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt cttcttcagg
     3841 tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc tttgccttcc
     3901 gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat atcaacgtca
     3961 ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc
     4021 agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag atcgttgtca
     4081 gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct gtttacggta
     4141 tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg
     4201 cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc aaccggaata
     4261 atcgctttca ggaagacagc aaagttttcg cgcagtttgg ctttgtcgat atctttgtac
     4321 agttccaggt gcttacggaa ctggtcgagg gtgtaccctt cttccgcacc tggcagacca
     4381 gcgatgatgt tacgggtcag acgcgctttg tcttcatcgc tcatggtggc gaagcgaaca
     4441 gcagcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg tttcaggata
     4501 tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga gccgtctggc
     4561 agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt gtagcacacg
     4621 gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc gatccactgc
     4681 tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gtacgctttc tacgacagac
     4741 cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc tacggaccat
     4801 acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg
     4861 acatcagcta aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg ttccatgatt
     4921 tttcctcttt cacatagtgg gttccacagc aaagagacgc ggaaccctgg agttgtttag
     4981 gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga tggcgtataa
     5041 gttctacctt ttggtaagac atgtcaaccc gaattctgaa attggttaac cacatcacaa
     5101 gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa gggtaatcga tggttattga
     5161 tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa
     5221 aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac attctttggg
     5281 taatattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc aattcaatgg
     5341 tggctttgtt ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat ttgatgtcgc
     5401 tgctgcacac catgaaagcg ggcttcggca acacgctggg ggaactggct atcatcgtgg
     5461 tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggctcac cagatagcac
     5521 atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt atcatcggcc
     5581 tgattttcgg tctggcgatg ttttatgaag tggcctttat catgttagcg ccgctggtta
     5641 ttgttattgc cgccgaagct aaaattccgt tcctgaaact ggcgatcccg gcagtagcag
     5701 ctgccactac cgcacattca ttgttcccac cgcagccggg tccggtggcg ctagtgaacg
     5761 cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg atcccaagtg
     5821 taatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat
     5881 cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc ggcgtttcga
     5941 ttctggtgcc gctgatccca gcgatcatta tgatctccac caccatcacc aatatctggc
     6001 tggtaaaaga tacccctgcg tgggaagtgg ttaattttat tggttcctcg ccgattgcga
     6061 tgtttattgc gatggtggtt gcattcgtac tctttggcac cgcgcgtggt catgacatgc
     6121 agtgggtgat gaacgctttt gaaagcgcgg tgaagagtat tgcaatggtg attctgatca
     6181 tcggtgcggg tggcgtgttg aagcagacca tcatcgacac cggcattggc gacaccatcg
     6241 gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg atcaccgtac
     6301 tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc gggattatca
     6361 gtgctgcaat ccttgatcca gcaactggtc agctggttgg cgtgaatccg gcgctgctgg
     6421 tactggcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc tcattctggc
     6481 tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg
     6541 agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tattagcatg gtagcgtaaa
     6601 aggacaccag agccagccaa tggcaggctc agactgatga caaagtctaa aaatgtgccc
     6661 ggacagtccc ctcgcccctc cggggagagg gttagggtga ggggaacaga ccagcaaagt
     6721 gcgctttgtt tatgccagat gcgacgctga cgcgtcttat ctggcctaca aagggctaac
     6781 gtgcaggttt ttagcttcag gtaatattgc gtaccagcat tagcaatgtc ctgtgatttc
     6841 tttattgata aacaaaagtc acgccaataa tcgattgcac attccctgca gtcacctgcc
     6901 ctccggtacg tgcataattt gccgttaatc ccagactcac cgccgaagtc cctactgctc
     6961 ctaaagatac cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga
     7021 cgccctgtgc aggtgaaaac gacgcggtat tggtgaaaat cgagttgccc gcatctgcgg
     7081 ttgtgccgga gaggtaatac cccaggtttt ggcttttcgc acaataaacg gtaagaggaa
     7141 ttggcactga accagggtag tccggcagag taacggtgac atcacgagca gaaacatcgc
     7201 agccgccagt aggcaccacc acatcattat tggcgtaaat attccacaca aactggaaat
     7261 catcgctgtt atagttgttg gtctgtcgca aaataagcac ggcaattaat gagccagctt
     7321 taatcgccac cccgcccgca ctgctcacag gcgtcaaata aagcgccacc ggccacggct
     7381 tatccgttct cgaattataa acaacgcgcg gcgtttcgct ggtggtagga aatggatagc
     7441 tactgccact atattttacg gtcccggaaa aattagataa cacgccgcca taagccgagc
     7501 ctcgttgcag tgtgacatag tctgtaatgg tttccggata atcgttatgg caaaagattt
     7561 gcgtcgaaag atccacgacc aggttttgcc ccacattcac ggcgggcgca aggtttacat
     7621 aaacattggc gctgccaccg ccaataggga tagcggtacc attggcggtt ttacaggcga
     7681 atgaccaggc atttaccgac cagcccatca gcagtacagc aaacagggta ataactcgtt
     7741 tcattacaat catctcttcg ggttcagctg taggtatagg tgatgctaat cactgcctga
     7801 atggttccct gagtggctcc gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc
     7861 gctgattgtg aggaatcatc cacctgaact gttttggttg cgccagtatt caatgtgttg
     7921 ccactgtcat cctgtagctc taactggatg ttttgcgcgg tcccctggtt tttataatat
     7981 ccggtgctgt cggctgcccc gctgaagctg gcagtgaccc tcgacgttcc caccggacaa
     8041 ttagtcaact caagcgcaac atcatgccag gccgatgccg ccccggcaga cataagactg
     8101 aaagaataaa gatcgccgag atcaaccgtg gcattggtgg tggaaactgt gcacggtttg
     8161 gcgacgacct taccgtttac cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg
     8221 agcgccaata ttgccgccaa taaataccca cgtttgcgcc atttcatgag catctccagt
     8281 tactgatatt caagagtgaa ggtagccgtg gcattgatat gccccgcagt gacaggcacc
     8341 tgtgtcgcca ttagccgggc gtaaaaattc aacgtatttg gtttacccgg cgtcagggtc
     8401 gtccacgaaa tcgcggacga tggagcatta aggggtattt gattttgctg ctcattcaga
     8461 agctgtattc ccagtcccga agccgctgac accgtatttt caagtgcaag cagattggca
     8521 ttgtggctat ctgcaacgcc ggtaaaccca acttttacgg cagaaacggc attaccacag
     8581 ggtgacagca aaatacgaaa tggaacaaca ggagtcgtcg cgccaatgtt gttaaattgc
     8641 ttcgccgcgt tttccatcag atcaacagta aaattggttg attcagcggc cacactacag
     8701 ccgttatctc tgacatagcc gcggatagta atcgtgctat ccgcagccaa agcgtgactc
     8761 accgccagcc acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat cacgccccct
     8821 taacgacatt cagctgatag ctgggttaat aactgctgct gactctctgg tggcagttga
     8881 taattggcga cacagtgagc attttcctct tctccccatt tcacctgaac ttttcccgct
     8941 aaaggcattc cgctgaggta aacctgacca ttatccgcaa caatgccgct actctggcta
     9001 ctctctgatg tcaccatcgc cccaaacggc agcggcttat tattgtgggt tagcgtcatg
     9061 agcagtttta tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc acgagtggga
     9121 acaacgttag cgaccgcgtt atctaaatcg acgttatcag ccagggtatt ggtatccagc
     9181 gccactctgt tttccctata ttcagtggca taaggcagca cggcataacc gcgccagtcg
     9241 gtacgcaccc ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc tttaacaagc
     9301 accaccgtat cgtttaacgg ctgccccagc gttacgccat tggcatgagc caatacccca
     9361 ccgctgactc cgtaatagag ctgcttaata tcatcgctat ggctgtaacc gatattggca
     9421 ttgccgtaac caccgcgata attcagcgtg gcgtagcctg tgcttccgct attaccatcg
     9481 cctcccccgg catagccggt ttgcacgcta tagctgaggt tgttgtcttc cagcaacgta
     9541 ccgtatacac cagccagatt ggtcatccga ccgttgagat cgtgtgacat gctgtagctg
     9601 gcactggcat gtcgccactg agatttactg tcagaacgca gccagtggct gaaaggaata
     9661 ttgacgttaa gcgctaacat ctgatcacgt cctttttgcc aggcgttttt cgtcaggcta
     9721 tagctgagcg tccagttgat atcttcgaat gcagtattta atccagcctg gaattgctca
     9781 tcgacattac tcgttcccca ataagtttga tggctaccac tcaaatacag tgttgatgtg
     9841 cgcccgagtt gctgagtaac ggtgagctgt aatttcccgc gtttgttata agcgaggttg
     9901 taatagtcgg tgaatttcgg cttaacctga ataactccgt cctgtgtttc gatgttgtag
     9961 ccattcattc gactgtatgt tgtatcagcg aaattaaaat atccgctggt cgaataacgg
    10021 taacccacta actgaatatt cgtgcctgac tcattgagcg atttgttata gagaaaacgc
    10081 accgattgtc cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc
    10141 acagacagag cgcccagtgc ccccatgttt ttcccgatac cgaaattaaa agcacgataa
    10201 cgatccgcca gttgcgttcc accatatatt gtccagccag ccggaaggcc gtggagtaat
    10261 gtactctgga aaaagcgggg tttttcctgc tgcgcatttc cactacggta ttctcctgcc
    10321 gtaatggaat aacgagtatg cccttcacgt tgcaaaagcg ggactgacga atagggtacg
    10381 gtaaaaatct gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc accactatta
    10441 cctgcggcat agatatcgtt gatggtaaaa ggccccggtg gcaccgtact attataaatg
    10501 tcatacccat tttgtttaat agtgacctgt gcagtaccac gagcaatacc gtggatcacc
    10561 ggggcaaatc ctctttggct atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg
    10621 cgaaagttaa taccatcgaa aatatcgccc tgagtataac catcacccag cgtcagccgg
    10681 gaacgtaacg gtattatgtc tcgctcaagc caggtattga tatgctgcca tttatttttg
    10741 ctacctgatg atctgtcgct actgttataa ctccaggtgg tattgtcgcg taaacgccac
    10801 gcaccaatat ttaacccact ctgtaggttt aaatatgcat aatggctgtt acccccaatc
    10861 cgattctgta cactatttcc gctgaaatta taattgagca atccggcatt aataccggga
    10921 tcccataact caggaggaat ataaccacgc gcgcgattac tcataaatgc ctgagggatc
    10981 gtcaggttca gtcgctgctg accaacatcc agatgcgcag tagcgtcctg gatcattgtg
    11041 gttaatggca cgcaggcatc atccgccagc agattcatac cggagacaga agccgtattc
    11101 agccccatac tggcaagttg cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg
    11161 cccgtattaa atgtgacatc acgcgttgcc atataaccat tattcaaata gatatcgaca
    11221 cgatacgtcc ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc ggccacagcc
    11281 tggggatcat ccgctaaaaa acgcgggtta aaatagagtt cggcagatga caaaggtgcc
    11341 tgtgcggcaa aagcacaggc aacaaagagc cggacaaaaa aaccagccaa acgatgctta
    11401 cgaatatgca agcattgtgt gtttcgctgg taaagtctta aattcagata tgacattatt
    11461 cctccggttt aaagtcaccc ggcagtcaat tctttttatt caggcgaaaa attccccctg
    11521 cgttattcca ttacgcccgt cattttgggg gtaagtgcac cataatcatt tattgttcgg
    11581 taagtaatat tgcttcccgc atcagaaggc aatttaaccg tgctttcgcc cattggaggc
    11641 accaaggcat tttcaagaac ccgggttccg gcattcaact ctgttaccgt caggtaatag
    11701 ggtgtcgggt taatcagcgt cagagaattc gcgctacgac gaaatcttaa tttttctgcg
    11761 gcctgatcgg gtggcaacgc taatttagcc gggcgatagt acagtttaat gcggctgata
    11821 attgcgagct gtagcgtatt ctcagtcaat tttgatttat ccattgacgg aatcgcttta
    11881 acattcatcc agaataaact ttcccgatcc tgtggcaatt ggttatttgt tgcatcaaga
    11941 atacgcaagg tattctcttt ttttcccttc atcgcaaaca gaggaggcgt cacgataaaa
    12001 cgaccatcct ttacaccatc ggcattttcc acccatgatt gaattaaata ggtactattt
    12061 tcatcattat ttgtcacggc aagttgcact tgtttttgcc cggccggata aattacgcga
    12121 gtcgcaccta aggccactcc cgcttcagcg cgtccggcaa ccatcattgc cataaacatc
    12181 aggatacctg ccagcaagca gaatgttatt tcctgcgatt tccttacatt gacgttttta
    12241 ttactcactg tcctgttcct gttatcacat tatctgctga acaattactg ataggctaaa
    12301 gagaaccagg cctgggcatt ggcgatgccg ccagtaaccc gacgcccggt agcacgatat
    12361 ttggcgatga aatgtagcga agtagagcct gagtaaagcc gtttccagtt tgctggagga
    12421 cgattaatcg gtacgaggtt tccttcatca tcaaacaacg ctacgccaat attggtggct
    12481 atccctggcc cctctcccac ggaaagcaca tccggatttt taccatccgc gacaccgtga
    12541 aacgccaccc ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa
    12601 ggcaccggtg cgctatcttc ccctgccgca tgaaaccggt tactgctgat ttgccccata
    12661 ttgaccgtca tttgtttatc accggcttca atccggcaag tttccgcaat aatgacgccc
    12721 tgaaattgca tatttccgcc aggcagcgtg gtattccatt tatttccggc cagagcaaac
    12781 attggcagca acgacgctaa cagaaataat cttttccttt tcatcgttat ttttatcgca
    12841 caagggtggg catccctgcc cgtaatgacg tccttgaacc tgggtaggtt attgatactg
    12901 aaccttgaag gttgcatccg cattagctgc acccggggtt gcctcgccga ttgcataata
    12961 acgcgcctgg aacggaatgg tgttggtacc gttattcagg gttgtttgtg cactaaatgt
    13021 cgcaccgtcc agcgtcagag cattgcctgt tctgtccagg atctgcacgc caacgtttgt
    13081 tgcactacct gcagcagaac tctgcagagc cagtacatca gtacgcgtag catcaattgc
    13141 cgtacctaag aaggcaacag cggcttttgt ggcaacagtg gtatcgcaat cattcagctg
    13201 aatgttaaaa ccaacggcag agctggttgc tccagcctgc ttcaggctag cggtacgaac
    13261 ctgtcccaac tgaacggttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac
    13321 aacttcccct ttaaagtgaa cggtcccacc atttaccgtc gtagtatcgg ccagagccgc
    13381 tgcggaactg agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct
    13441 gctttccttt aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt
    13501 ttgtcaacga gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg
    13561 ggccattttg actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgttggc
    13621 ttaatattct at
//
LOCUS       DAZGCS010000121        13202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGCS010000121 DAZGCS010000000
VERSION     DAZGCS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.123"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            476..2107
                     /gene="oppA"
                     /locus_tag="ACHK01_004069"
     CDS             476..2107
                     /gene="oppA"
                     /locus_tag="ACHK01_004069"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO8098935.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEARRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2193..3113
                     /gene="oppB"
                     /locus_tag="ACHK01_004070"
     CDS             2193..3113
                     /gene="oppB"
                     /locus_tag="ACHK01_004070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO8098936.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3128..4036
                     /gene="oppC"
                     /locus_tag="ACHK01_004071"
     CDS             3128..4036
                     /gene="oppC"
                     /locus_tag="ACHK01_004071"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO8098937.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4048..5061
                     /gene="oppD"
                     /locus_tag="ACHK01_004072"
     CDS             4048..5061
                     /gene="oppD"
                     /locus_tag="ACHK01_004072"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO8098938.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5058..6062
                     /gene="oppF"
                     /locus_tag="ACHK01_004073"
     CDS             5058..6062
                     /gene="oppF"
                     /locus_tag="ACHK01_004073"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO8098939.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6115..6444)
                     /gene="yciU"
                     /locus_tag="ACHK01_004074"
     CDS             complement(6115..6444)
                     /gene="yciU"
                     /locus_tag="ACHK01_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO8098940.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6479..7939)
                     /gene="cls"
                     /locus_tag="ACHK01_004075"
     CDS             complement(6479..7939)
                     /gene="cls"
                     /locus_tag="ACHK01_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO8098941.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8082..8255
                     /gene="yciY"
                     /locus_tag="ACHK01_004076"
     CDS             8082..8255
                     /gene="yciY"
                     /locus_tag="ACHK01_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO8098942.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8310..9563)
                     /gene="kch"
                     /locus_tag="ACHK01_004077"
     CDS             complement(8310..9563)
                     /gene="kch"
                     /locus_tag="ACHK01_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO8098943.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9863..10159)
                     /gene="yciI"
                     /locus_tag="ACHK01_004078"
     CDS             complement(9863..10159)
                     /gene="yciI"
                     /locus_tag="ACHK01_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO8098944.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10383..11102
                     /gene="tonB"
                     /locus_tag="ACHK01_004079"
     CDS             10383..11102
                     /gene="tonB"
                     /locus_tag="ACHK01_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO8098945.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11142..11540)
                     /gene="yciA"
                     /locus_tag="ACHK01_004080"
     CDS             complement(11142..11540)
                     /gene="yciA"
                     /locus_tag="ACHK01_004080"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO8098946.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11645..12184)
                     /gene="yciB"
                     /locus_tag="ACHK01_004081"
     CDS             complement(11645..12184)
                     /gene="yciB"
                     /locus_tag="ACHK01_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO8098947.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12214..12957)
                     /gene="yciC"
                     /locus_tag="ACHK01_004082"
     CDS             complement(12214..12957)
                     /gene="yciC"
                     /locus_tag="ACHK01_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO8098948.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12985..13074)
                     /locus_tag="ACHK01_004083"
                     /pseudo
     CDS             complement(12985..13074)
                     /locus_tag="ACHK01_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3560 a         3121 c         3223 g         3298 t
ORIGIN      
        1 aattgttaaa ttattgtgca tttcactact ggaactgtaa tcagaaaaga tagacatgct
       61 tagccaatct ctatttgatt gaattgaaag atgtttgtta aggcatggat gcaagctata
      121 gattctgata cggtcaataa aagagaattg cttaacaatt ttgcaaaatg tattggcgag
      181 taagaaccgc atttggtact ttccgggcaa ccgccagacg attctttatt ggtaatgaga
      241 ataattaaca attaaagagc gtcgcgaaag aataatgtgt ctcgacaggg gagacacagt
      301 acgaatcgac ataaggtgat cgtctgaatc accagaataa ataaagtcgg tgatagtaat
      361 acgtaacgat aaagtaacct gacagcagaa agtctccgag cctgtgcagg gtcccaatcc
      421 gggattacac atgctggtta ataccagtaa ttataatgag ggagtccaaa aaacaatgac
      481 caacatcacc aagagaagtt tagtagcagc tggcgttctg gctgcgctaa tggcagggaa
      541 tgtcgcgctg gcagctgatg tacccgcagg cgtcacactg gcggaaaaac aaacactggt
      601 acgtaacaat ggttcagaag ttcagtcatt agatccgcac aaaattgaag gtgttccgga
      661 gtctaatatc agccgagacc tgtttgaagg cttactggtc agcgatcttg acggtcatcc
      721 agcacctggc gtcgctgaat cctgggataa taaagacgcg aaagtctgga ccttccattt
      781 gcgtaaagat gcgaaatggt ctgatggcac gccagtcaca gcacaagact ttgtgtatag
      841 ctggcaacgt tctgttgatc cgaacactgc ttctccgtat gccagttatc tgcaatatgg
      901 gcatatcgcc ggtattgatg aaattcttga agggaaaaaa ccgattaccg atctcggcgt
      961 gaaagctatt gatgatcaca cattagaagt caccttaagt gaacccgttc cgtacttcta
     1021 taaattactt gttcacccat caacttcacc ggtgccaaaa gccgctatcg agaaattcgg
     1081 cgaaaaatgg acccagcctg gtaatatcgt caccaacggt gcctatacct taaaagattg
     1141 ggtcgtaaac gaacgaatcg ttcttgaacg cagcccgacc tactggaaca acgcgaaaac
     1201 cgttattaac caggtaacct atttgcctat tgcttctgaa gttaccgatg tcaaccgcta
     1261 ccgtagtggt gaaatcgaca tgacttataa caacatgccg atcgaattgt tccagaagct
     1321 gaaaaaagag atcccggacg aagttcacgt tgatccatac ctgtgcactt actattacga
     1381 aattaacaac cagaaaccgc cattcaacga tgtgcgtgtg cgtaccgcac tgaaactagg
     1441 tatggaccgc gatatcattg ttaataaagt gaaagcgcag ggcgacatgc ccgcctatgg
     1501 ttacactcca ccgtatactg atggcgcaaa attgactcag ccggaatggt ttggctggag
     1561 ccaggaaaaa cgtaacgaag aagcgaaaaa actgctggct gaagcgggtt ataccgcaga
     1621 caaaccgttg accatcaacc tgttgtataa cacctccgat ctgcataaaa agctggcgat
     1681 tgctgcctct tcattgtgga agaaaaacat tggtgtaaac gtcaaactgg ttaaccagga
     1741 gtggaaaacg ttcctcgaca cccgtcacca gggtactttt gatgtggccc gtgcaggctg
     1801 gtgtgctgac tacaacgaac caacttcctt cctgaacacc atgctttcga acagctcgat
     1861 gaataccgcg cattataaga gcccggcctt tgacagcatt atggcggaaa cgctgaaagt
     1921 gactgacgag gcgcggcgca cagctctgta cactaaagca gaacaacagc tggataagga
     1981 ttcggccatt gttcctgttt attactacgt gaatgcgcgt ctggtgaaac cgtgggttgg
     2041 tggctatacc ggcaaagatc cgctggataa tacctatacc cggaatatgt acattgtgaa
     2101 gcactaatgg caatacgtgg ggcaggagtg tcctgctcca cggtgtctga tttttatcgc
     2161 attacagaag gcacaggcca gaaggtaggg caatgttaaa atttattcta cgtcgctgtc
     2221 tggaagcgat tccgacgcta tttattctta taactatttc gttctttatg atgcgcctcg
     2281 cgccgggaag cccttttacc ggcgaacgta ctttaccgcc agaagtgatg gccaatatcg
     2341 aagcgaaata tcatcttaat gatccgatca tgacacagta tttcagctac ctgaaacaac
     2401 tggcgcacgg tgatttcggt ccatcgttta aatataaaga ttattcggtc aatgacttgg
     2461 ttgcatccag ttttcccgtt tctgccaaac tgggagccgc agcatttttc cttgcggtaa
     2521 tactgggtgt tagtgctggc gttattgccg cattaaaaca aaacaccaaa tgggactata
     2581 ccgtgatggg gctggcaatg accggggttg ttatccccag ttttgtggtt gcaccattat
     2641 tagtcatgat atttgcgatc attttgcatt ggctgccggg cggtggctgg aatggtgggg
     2701 cgcttaaatt catgatattg ccgatggtgg cgttgtcact cgcttatatc gccagtattg
     2761 cgcgtattac ccgtggctct atgattgaag tattacactc caattttatt cgtactgccc
     2821 gggcgaaagg gttacctatg cggcggatca ttttacgtca cgcattaaaa cctgctctgt
     2881 tacccgtgct ctcttatatg ggccccgcat ttgtcggcat tattaccggt tctatggtta
     2941 tcgaaaccat ttatggtttg ccggggattg gacaactgtt cgttaatggc gcattgaacc
     3001 gtgactattc cttagtgtta agcctgacca tcctggttgg tgctttaacc attttgttta
     3061 atgccattgt cgatgtgcta tatgcggtta tcgacccgaa aattcgttac tgatactgga
     3121 gctcgcgatg atgttaagta agaaaaacag cgagacgctg gaaaatttca gtgaaaagct
     3181 ggaggtcgaa gggcgcagct tgtggcagga cgcacgtcga cgttttatgc ataaccgtgc
     3241 ggcggttgcc agtctgatag tgctggtgct gatcgcgtta tttgtaatcc tggcaccgat
     3301 gctttcccag tttgcctatg acgatactga ctgggcgatg atgtccagcg ccccggatat
     3361 ggagtccggt cactactttg gtactgactc atccggtcgc gacctgcttg tgcgcgttgc
     3421 gattggcggg cgtatctcac tcatggtcgg tgttgctgcg gcactggtgg ctgtggtcgt
     3481 cgggacactt tatggttcgc tttccggtta tctgggcggt aaagtggatt cggtaatgat
     3541 gcgtctgctg gaaatcctca actccttccc attcatgttc ttcgtcattt tgcttgtgac
     3601 ctttttcggt caaaacatcc tgctgatttt cgtggcgatt ggcatggttt cctggctgga
     3661 tatggctcgt attgtgcgtg ggcaaaccct gagtctgaag cgcaaagagt ttattgaggc
     3721 ggcacaagtt ggcggtgtat cgacatcggg cattgttatt cgccacattg tgccgaatgt
     3781 actcggtgtg gtggtggtct acgcatcact tctggtgccc agcatgatcc tctttgaatc
     3841 tttccttagc ttcctggggt tgggtacgca agagccgtta agcagctggg gtgcattgct
     3901 gagtgatggc gcgaactcga tggaagtctc tccatggtta ttgttgttcc cagcgggatt
     3961 cctcgtggtg acgctgtttt gtttcaactt tatcggcgat ggcttgcgtg atgccctcga
     4021 cccgaaagat cgttaaggag tgcagccatg agcgtaattg aaactgcaac tgtgccgctc
     4081 gcacaacaac aggctgacgc actgctgaac gtgaaagatt tgcgtgtcac ctttagtacc
     4141 ccggacggcg acgtcacggc ggtcaatgat ttgaattttt ccctacgtgc cggagaaacg
     4201 ctgggtattg taggtgagtc tggttcgggt aaatcgcaaa ctgcatttgc gttgatgggc
     4261 ctgttggctg ccaacggacg tattggcgga tcggcaacct tcaatgggcg tgaaatcctc
     4321 aatttgccag agcatgaact caataaactg cgcgctgaac aaatctcaat gatttttcag
     4381 gacccaatga cttcgttgaa tccctatatg cgcgtcggtg agcagttaat ggaagtgctg
     4441 atgctgcata agaacatgag caaagctgag gcgtttgaag agtcggtgcg gatgctcgat
     4501 gcggtaaaaa tgccggaagc gcgtaaacgc atgaaaatgt acccgcacga attttctggc
     4561 ggcatgcgtc agcgagtcat gattgcgatg gcattgctat gtcgacctaa gctactgatt
     4621 gcggatgaac ccactactgc gctggacgtc accgtacagg cgcagatcat gacgctatta
     4681 aatgaactga agcgggaatt taataccgcc atcattatga tcacccacga tcttggcgtg
     4741 gtggcgggga tctgcgacaa agtgctggta atgtacgccg ggcgcacgat ggaatatggc
     4801 aatgcgcgcg atgtctttta tcaacccgtt catccttatt ctatcggttt gctcaacgcg
     4861 gtgccgcgtc ttgatgcgga aggtgaaaca atgttgacca tcccgggtaa tccgccaaac
     4921 ttgctgcgat taccgaaagg ttgcccgttc cagccacgtt gtccacatgc gatggaaatt
     4981 tgtagtagcg caccgccgct ggaagagttt acgcctggcc gtctgcgtgc ttgctttaaa
     5041 ccggtggagg aactgttatg aatgctgtaa ctgaaggaag aaaagtcctc cttgaaatcg
     5101 ccgatcttaa agtgcacttt gaaatcaaag atggcaaaca gtggttctgg caaccgccga
     5161 aaacgctcaa agccgtcgat ggtgtcactc ttcgcctgta tgaaggggaa acattaggtg
     5221 tggtagggga atcgggatgc ggtaagtcca cctttgctcg cgccatcatt ggcttggtta
     5281 aagcgaccga cggtcatgtt gcctggttag gtaaagagtt gctgggcatg aagcccgatg
     5341 aatggcgtgc cgttcgcagt gatattcaga tgattttcca ggatccgttg gcatcgctaa
     5401 acccgcgtat gaccatcggc gagatcatcg ctgaaccact gcgtacttat catccgaaaa
     5461 tgtcacgcca ggaagttcgc gagcgcgtga aggcgatgat gctgaaagtc gggttattgc
     5521 ctaacctgat taaccgctat ccgcatgagt tctccggtgg gcagtgccag cgtatcggga
     5581 ttgctcgtgc tcttattctt gaaccgaagc tgattatctg cgatgagccg gtttcggcgc
     5641 tggacgtgtc aattcaggcg caggtggtca acctgctcca gcagctgcaa cgtgagatgg
     5701 gattgtcatt aatttttatc gctcatgacc tggccgtggt aaaacacatt tccgatcgtg
     5761 tgttggtgat gtatctcggc catgcggtag aactggggac ctatgatgag gtctaccaca
     5821 atccactaca tccttacacc agggcattga tgtcggcagt ccccatacct gatccggatc
     5881 tggagaagaa caaaaccatc cagttactgg aaggggaatt accgtcgccg atcaacccgc
     5941 cttccggttg tgttttccgt acccgttgcc cgattgccgg tccggagtgc gccaaaacac
     6001 gtcctgttct ggaggggagt ttcagacacg ccgtttcttg cctgaaagtc gatccgcttt
     6061 aaacacaata agggctgaca gttgtcagcc ctttttcacg ctaaaagcga ttatttattc
     6121 ccgccagatg atatggcaaa gtttgtgatc tttttcgcgg cataacagaa tgcgggcgaa
     6181 aacatcgttg atttcaccat cttcactgtc cgccagacca atcaccactt cggcaaaaaa
     6241 gtctgggttc aaatcgaaat caacatgttc ctgccagtct tccgccggat cgaataactc
     6301 cgcgccgccg cgctcttcaa actgaagatt gaacagcaga acatcagctg gatcgagatt
     6361 gtcggcagcc agttcgagaa aaatatcgta ggcctgctca agcgtttcat cttcagtcag
     6421 gcgattgttt agatccatat ccatagtcac tacctgttta acctctgttg gcgacgtttt
     6481 acagcaacgg actgaagaag taaaacagtc gctcggcgac acgttgccat aatggacgtt
     6541 ttagccataa acgggcatcg agcagacgtg aacgcgaaat ataatcgtcc tgaacggcgg
     6601 caaggtctgc accaaaacct ttatcgtcga ttgccagggt aatctcgaaa ttcagccaca
     6661 gactacgcat atcaaggtta acggtgccaa ccagacttag ttcgccatcg accagcacgc
     6721 tcttggtatg cagtaacccg ccttcaaact gataaatttt aaccccagca gcaagaagtt
     6781 ccgtaaagaa tgcgcgactg gcccagccga ccagcatcga gtcatttttt cgcggaagga
     6841 taatactgac atccaccccg cgctgcgccg ccgtgcaaat cgcatgaagt aaatcatcgc
     6901 ttggcacaaa gtagggcgtg gtcatgatca aatattcacg cgccgaataa gccgcagtca
     6961 ataatgcctg gtgaatgaga tcttccggaa agccggggcc agaagcaatt gtgtgaatgg
     7021 tgtgaccgct ggcctgttca aacggcataa tattgacatc tggtggtggc ggcagaatac
     7081 gttttccggt ttcaatctcc cagtcgcagg aataaataat ccccatcgcg gtggcgatgg
     7141 ggccttccat acgcgccatc agatcaatcc attgccctac gcccgcatct tgtttgaagt
     7201 agcgaggatc gaccatattc atgctgccgg tgtacgcgat gtaattatcg atcatgatca
     7261 tcttgcgatg ttggcgcagg tccatacggc gtaaaaacac acgcatcaga ttgaccttta
     7321 aggcttcgac cacttcaata ccggcattac gcattagctc gggccacggg ctgcggaaaa
     7381 aagccacact cccggcggag tcgagcatca atcggcaatg aataccacgc cgtgcagccg
     7441 ccattaatga ttcagccacc tggtccgcca tgccgccggg ctgccagata taaaacacca
     7501 tctcaatatt atggcgcgcg agctggatgt cgcggattaa cgcctgcatc acatcatctg
     7561 actcggtcat cagttgtagc tgattccctt tgaccccagc gatcccctga cgacgctcgc
     7621 aaagcttgaa taatggcgca gcgacactgc tattttcttc ggcgaagata tgcttacagg
     7681 ctttaaggtc gttaagccat tttgcggtgg aaggccacat cgctctggcg cgctcagcgc
     7741 ggcgtttgcc taaatggagc tcgccaacgg caagataggc aataattccg actaacggca
     7801 gaatgtaaat aatcaacagc caggccatcg cggagggaac tgcgcgtcgt ttcattagaa
     7861 tgcgtaaagt tacgcctgca atgagcaacc agtatcccag aatggccaac caactcacca
     7921 acgtataaac ggttgtcata gattagaaat ccttttgaaa gcgcattgtt atgagtttac
     7981 gcatatggat tcatctggca aataaaaacg cgggaaaagc actggtcagc gtcgggggtg
     8041 ggggtataat gaccattctg ttattgcata gagtagttaa catgaagcgg agtagaacgg
     8101 aagtggggcg ctggcgcatg cagcgtcagg ctagccgacg taaatcgcgt tggcttgagg
     8161 ggcaatcgcg ccgaaatatg cgtatccaca gcatcaggaa gtgcattcta aacaaacagc
     8221 gtaactcgtt attgtttgcg atctacaata tctaaatgta ctgggcacca ttgcggtgcc
     8281 caataagttt actcttccgt ctttaaactc tatttttgcg ccgattcttt actgtcagct
     8341 ttcgtttctt gttcatcgtt atcgctgaaa ataccatgac ctgagttcaa caacattgaa
     8401 acgagcatat cattattaat ctcttcacca tttaaaactc gcgccagaat ttcgctgcca
     8461 aacagttgcg gtgagagaat gatatccgga tgtaccatct taatcttatt caggtttttg
     8521 ctatcactga cggcgagaac tgttttgaca tcactgctca tatcttttgc cgagagtaca
     8581 acaaacgcgt tatctgcatc gttatcactc agcgccagaa tggcccggca acgatcgatt
     8641 cccgctttct ttaatactga actgtcatta ctgtcaccgg ggataacatc agcgttatcg
     8701 cctaagcgtt gctcaagttg cttgatatca tcttcaggca agttgctgat aaccgttacg
     8761 ttttgtccgc gttgattcag ttgcaggatc gtattgatgg cgagaatcga atgtccgcaa
     8821 acaataaaat gatctttacg atgcattgta tgattgtttc cttttacaag tttgttgaat
     8881 cccccgcgga taagcggacc aaaaattgag gtcatagatg tggcaaaaac ggtaatgccg
     8941 gaaataatga ccgaaatagt gaacaatcgt gctgattcag aaacagggac aatatcgcca
     9001 tagccgacgg ttgacatggt ttctatcgaa aaatagaacg ccgtcatcaa actttctatt
     9061 cgcggattaa aaccttcgct taaataaagc gcaccgtagg ttgagtaaaa cagtaatgtc
     9121 gtgaaactaa taaatgcaaa aattgtcccg gctgcggcgc tactgtggga gaagtctttg
     9181 cgcagtatca gcaaaaacac cagcgtaaaa atgcaaaatc caatactaaa tttcagccag
     9241 ggataaaaat gcagggtgta aattagcgct atcaacaaca aaatgatact gattgcccat
     9301 gcgagctttg cccggaacag taagccaatg gagttcagga caagaaagac gccgagcata
     9361 aatagtgggg cgtttgccag caaggagaga tccaactctg aaaatgcttt gatatggaaa
     9421 atatcaagca aattgaccga cataccatag attgttttaa aaataagcaa tccatttaaa
     9481 aagacggcca gcgcgagaat gtcgtgccga agggtcaccc ataaatttgt tgctgtttgt
     9541 ttgaatgtag cccagtgact cactgatacc tctgtaacca caattttgtt taattatctt
     9601 agcgtgcaca ttttctgaac atacatgcag cgcgtgaatg tgttaaacaa tagtagatga
     9661 ctttatccgc agtaatcaaa ggatttgagc gagcaactgt acctcaggaa aggtcatttt
     9721 tcctgaatat gctcacatca tataaagaaa tacagataaa gttattatct gcttgtggta
     9781 gtgaatgcac tgaccggcta taaggaaagg ccaaacaaga acacggttgc aaaaaccgtg
     9841 cccttaaata ttgaatctct attcagaaca ctttcttaaa tggtttcact gaaacgtgtt
     9901 catagactcc tgccgctacg tacgggtcag catcggccca ggcctgagct gcttccagcg
     9961 attcaaattc agcaataacg gttgagccag taaatcccgc agcccctgga tcgttactgt
    10021 ctaccgctgg cattggacca gctgtcaaca aacgaccttc atcatgcagt aactgtaaac
    10081 gtgctaaatg tgccggacga acggaaaggc gtttttcgag gctatcagct ttgtcttgag
    10141 cgtaaataac atacaacacg ggcacaactc cttgttcggg aaagttgtaa agtacgttat
    10201 ttgaaagggc gaagatctgc aacggaaaga tgatgtcttt gttaagacca tgcataaatt
    10261 aaggtcaatt acgccaaaaa tgacattttc actgatcctg atcgtcttgc cttattgaat
    10321 atgattgcta tttgcattta aaatcgagac ctggtttttc tactgaaatg attatgactt
    10381 caatgaccct tgatttacct cgccgcttcc cctggccgac gttactttcg gtctgcattc
    10441 atggtgctgt tgtggcgggt ctgctctata cctcggtaca tcaggttatt gaactacctg
    10501 cgcctgcgca gccaatttct gtcacgatgg ttgcgcctgc tgatctcgaa ccgccacaag
    10561 ccgttcagcc gccaccggag ccggtggtag agccagaacc ggaacctgag ccgatccccg
    10621 aaccaccaaa agaagcaccg gcggtcattg aaaagccgaa gccgaaacct aagccaaaac
    10681 cgaagccggt gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc gtagagtcgc
    10741 gtccggcatc accgtttgaa aatacggcac ccgcacgcct gacatcaagt acggcaacgg
    10801 ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta agccgtaatc
    10861 agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt aaagttaaat
    10921 ttgacgtcac gccggatggt cgcgtggata acgtacaaat cctctcagcc aagcctgcga
    10981 acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc
    11041 caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcaccacc gaaattcagt
    11101 aagcagaaag tcaaaagcct ccgaccggag gcttttgact attactcaac aggtaaggcg
    11161 cgaggttttc cttcaggatc aaccgcgaca tacttaaata atgcttctgt cgctttatag
    11221 cgttgcccga ttggttcaga cgctactttt ttcacccaca cttcaatatt aatgctgacc
    11281 gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc
    11341 ggccgtaaga aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg ggcaatttct
    11401 ttcgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc aaagatgtca
    11461 ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc gccctgaggg
    11521 acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct
    11581 actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag gactggcagt
    11641 ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg cttaacaatg
    11701 tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa atattttgcg
    11761 gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa acagcccaag
    11821 ccagatttag cttcgaccat accggttgcg gcagcgtgag ttctttaccc agcatccgct
    11881 gaattagcgg ctttttcatc acccattggc tgactaacag ggctcccgca aacagagcat
    11941 aaatgactgt aaccttccat ttaataaact catcattgtg gaagaacagc gtcaagccac
    12001 cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta cgaaagcgaa
    12061 cccagctata gataagcaca atcgccgtgg cgacgatcag cgccgcagta gccgcataga
    12121 tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca agaaactgct
    12181 tcattttacg attccgtaat caaatgctta aggttattgg cgaatcaaca tatacaggcg
    12241 gaacagatag atgagcaata tggcagaaat caggttgctc aaggtgttcg ccagtacggc
    12301 accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca gtgtttttgc
    12361 cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccaag tcagccgcat
    12421 actgctacgc atcgaggcaa aaatgcccat tttgtcctgt accagcatca ccggtgccag
    12481 ggccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc caatctgtac
    12541 taaaagggta gtcagaaaaa tcagaataaa taacttgggc aatatcggcg cactggcacc
    12601 aatagctcgc agcgcactga ctctctgacc cgcagacacc agctggataa ttaatattac
    12661 gcctccggcg agaatggcgt taccgattaa tcctgaaaaa gtggacgccg ctgaagcctg
    12721 cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca acccactact
    12781 gccgctaacg ggcacgccgt cattgagctg cgcaagctgt gcatcactgg gtgagaaaac
    12841 atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa tggtcataaa
    12901 ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga tagacatgca
    12961 ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg ttaagcctcg
    13021 ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag gtaattgctg
    13081 gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg cgtagagaaa
    13141 atatttaaaa attgatttaa atcacattaa ccaggattct caatgcaact tctaaattaa
    13201 tc
//
LOCUS       DAZGCS010000122        13196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGCS010000122 DAZGCS010000000
VERSION     DAZGCS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.124"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..466
                     /locus_tag="ACHK01_004084"
     CDS             <1..466
                     /locus_tag="ACHK01_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8098949.1"
                     /translation="SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLE
                     MVICTGSNMAKVDFNDANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILR
                     GEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAAAMKITADIMQHAD"
     gene            complement(619..2268)
                     /gene="yjcE"
                     /locus_tag="ACHK01_004085"
     CDS             complement(619..2268)
                     /gene="yjcE"
                     /locus_tag="ACHK01_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO8098950.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2419..3768)
                     /gene="ghxP"
                     /locus_tag="ACHK01_004086"
                     /gene_synonym="yjcD"
     CDS             complement(2419..3768)
                     /gene="ghxP"
                     /locus_tag="ACHK01_004086"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO8098951.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4314..4778)
                     /gene="soxR"
                     /locus_tag="ACHK01_004087"
     CDS             complement(4314..4778)
                     /gene="soxR"
                     /locus_tag="ACHK01_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO8098952.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4864..5187
                     /gene="soxS"
                     /locus_tag="ACHK01_004088"
     CDS             4864..5187
                     /gene="soxS"
                     /locus_tag="ACHK01_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO8098953.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5190..6776)
                     /gene="pdeC"
                     /locus_tag="ACHK01_004089"
     CDS             complement(5190..6776)
                     /gene="pdeC"
                     /locus_tag="ACHK01_004089"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO8098954.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7206..7487
                     /gene="yjcB"
                     /locus_tag="ACHK01_004090"
     CDS             7206..7487
                     /gene="yjcB"
                     /locus_tag="ACHK01_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO8098955.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7586..8122)
                     /gene="ssb1"
                     /locus_tag="ACHK01_004091"
     CDS             complement(7586..8122)
                     /gene="ssb1"
                     /locus_tag="ACHK01_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO8098956.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8376..11198
                     /gene="uvrA"
                     /locus_tag="ACHK01_004092"
     CDS             8376..11198
                     /gene="uvrA"
                     /locus_tag="ACHK01_004092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO8098957.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11233..11589)
                     /gene="yjbR"
                     /locus_tag="ACHK01_004093"
     CDS             complement(11233..11589)
                     /gene="yjbR"
                     /locus_tag="ACHK01_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8098958.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11593..12009)
                     /gene="yjbQ"
                     /locus_tag="ACHK01_004094"
     CDS             complement(11593..12009)
                     /gene="yjbQ"
                     /locus_tag="ACHK01_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO8098959.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12120..12833)
                     /gene="aphA"
                     /locus_tag="ACHK01_004095"
     CDS             complement(12120..12833)
                     /gene="aphA"
                     /locus_tag="ACHK01_004095"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO8098960.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3435 a         3452 c         3212 g         3097 t
ORIGIN      
        1 ctctaaactc gatttaatta actttgaaca tgctgatctg tctcgcgtca atctgaacaa
       61 agcaatcctc cagaatataa acttaattga tagcaaactc ttttgtacgt ggctgacaaa
      121 tacattcctc gaaatggtta tatgtaccgg ctctaatatg gctaaggttg attttaatga
      181 tgccaattta agcaactgcc atttcaactg ttctatttta acaaaagcct gtatgtttaa
      241 tacccgtctc tatcgggtta atttcgatga ggctagcgtc caaggaatgg gcatttccat
      301 tctccgtggg gaggaaaata tccccattga tagtgatacc ctggtaacac tacagaaatt
      361 ctttgaagaa gattgtacct ctcatactgg catgtcacaa actgaggata atattaatgc
      421 agccgctatg aagattactg cagatattat gcaacacgca gattgaggtt aagtcgaaga
      481 acagccaatg aattgacttt acaagaggat aacttatttg ttttagaatc atgaatgtta
      541 gcccgagcta acctgttaaa ggtgcaaaag attgcacctt tttcagatta cctcgggcct
      601 ggttacgtcg ctttcggctt actgattttc ctcaattagc aacgcttcaa gcaaatcgag
      661 atcgtgcagt aatttttgca gcgtttcgtt gctgatctcc cgcgtggcgc gcaggtggta
      721 cagttcagca cgttcagaac gcaatgccgc cagacggaag cgacgctcaa ggttctcttc
      781 ctgcacggaa ctttcaacgt cgttacgtcc atcggcgcga cgacgcaggt taccaatgac
      841 gcgagaactg acctccgtaa gcagctggtt atcgatattc tcttcggtat ccgccgccag
      901 acgctcctcc attttctgga tcgccacaat cgccacttct gccgttgccg ctcgcgcaat
      961 acgttcttct ttcaattgtt gcgaatggtc cgcgacttca atgtgttgta gcagaatagg
     1021 caacatcacc acgccgacaa acagcgagaa gagaatgacg ccagccgcca ggaacaccag
     1081 ctcatagcgc gccgggaaga cgttaccatc cggcaagagc agcgggattg agagcacacc
     1141 ggccagagtg atcgccccac gcaccccggc gaaagacgcg atcaggattt ctcgtgtggt
     1201 ccacgaacca aactccatcg gcttcttctt caggaagcgg ttgctgaact ttttcatcgt
     1261 ccacagccag ccgaaacgga ccagcatcag cgccgcatat atcagaataa tatcggtaaa
     1321 cagcatccag atttcgacgt tagggtcgat ttctgctgcc atcagcgacg tctccagaat
     1381 acccggcagc tgcagaccta acagcaggaa caccatgccg ttaaagacaa attccagcat
     1441 cgcccaggtg ctgtttgcac gcaggcgcat tgccagcggc gcacggcgca tcacaccgga
     1501 gcgggtgatg gtcatcccgg cggcaaccgc agcgaggatg ccggaaacgc caatatgttc
     1561 ggcaatcaga taagaagcga atggcagcaa gaacagcaga acaatctgcg tcgcgggttc
     1621 atcaccgccc cagcggctga ggaatcgcag cgagcgacca tacagccagc tcaccacaaa
     1681 accggcaaga ataccgccaa tggctacttt cataaattcg accgtcgcac cgccgacggt
     1741 gaagatcatc gtccccatcg ccactgccac ggcaaacttc aacgacacca ggccggaagc
     1801 gtcattcatc aacgcttcgc cctgcaaaat gcccatgatt tttttcggga tgcgcccttc
     1861 gccaacaatc ccggagagcg ccacagcatc ggtcggagaa agtaccgccg ccagcgcaaa
     1921 ggcggggatc agcggaatgc ccggcaccac ccagtaaata aggaagccaa tgccgaccac
     1981 ggtgaccacc accagcgcca gcgcgaggcc gaaaatctct cgaccatgtt cgagaaattc
     2041 acgggtcggc gttttccagc catcagcgaa cagcaacggc gggataaaca agactaaaaa
     2101 gagttcagga tcaaactcca catgcaaacc aaacgtcggc cacgccagta gcgcaccgat
     2161 ggcgatttgc ataagcggaa gcgggatctg aaagggcatg acacgagtga ccaccccgga
     2221 cagcgagacc acgagggtca ttatcagtat ggtgaagaag atttccatgc gttccctgtt
     2281 tgcgatgttt tttatgatct gagaaagacg ttatgcctga attctcattt tttcagaata
     2341 tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg gcagccattg ccacccgttt
     2401 tcagaatccg atgaaagatt agatagccca gccacccgca tagaaggtca ccagcgccac
     2461 ggcgataacg accgtaccga tgttcaactt gcgccattcg ccggaaacca gacgaccaat
     2521 caccagagtc gcgaagccga tcatgatgcc tgttacgatg ttacaggtca gcacgatgaa
     2581 taccgccgta accagacccg ccatcgcatc aacaaaatca gcaaagtcga ttttcgccac
     2641 gttgctcagc atcagcaggc caacgtacat cagcgccgga gccgttgcat accccggaac
     2701 gaggtaagag agcggagaga ggaacagaat cagcaggaac agcacgccaa cggtgatagc
     2761 cgtcaaaccg gttttaccgc ccgccgccgt acccgccgca gactcgatgt ataccgctgc
     2821 cggagctgca cccaccaggc cagagaaaac gctgctcatg gagtcagtgg tcagtgcttt
     2881 cccaccgtcg ataatttgcc catctttatc cagcaggttc gcctggccgg cgacggcacg
     2941 gatagttccg gtggcatcaa atactgccgt cattaccagc gccagaacgc ttggcaggac
     3001 tacaggattc agcgcgccca taatgtccag gctgccaatc agtgagttgc cgttttcatc
     3061 gctcaatgaa ggcatggcga aaacgccgga gaaatggacg ttaggatcga agatcaaacc
     3121 gacaattgag ataccgataa tggtcagcag aatgccacca gggactttca gtttttccag
     3181 gccgatgatc accgccagac ctaccagtga cataatcacc gggaaggtcg cgaaatcacc
     3241 cagcgcaacg ggcagaccat caagcgggtt tttaatcacc agaccgacac cgttagcggc
     3301 aatgagcagc aggaacagac cgataccaat ccccgtgccg tgcgccacac cgtgcggcaa
     3361 gttgcgcaaa atccagctac ggatacccgt ggcagaaatt accgtaaaca gcacacccat
     3421 caggaacacg gcacccagcg cgacaggtac gctaatatgt tgccccagca ccaggctgaa
     3481 tgcggtaaac gccgtcaggg agatggcgca accaatcgcc aacggcagat tagcccacag
     3541 acccatcacg atagaaccga gtccggcaac cagacaggtt gcaacgaaaa ctgccgcagg
     3601 cgggaagcct gctttaccca acatacctgg aacgacgatg accgagtaga ccatcgccag
     3661 aaacgttgtt aacccggcaa ccacttcctg acggacagtg cttccacgtt gtgaaatttt
     3721 aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc gtagacataa gaaaacatcc
     3781 cctgagagtt taattttcgt caagatgcgt ggcggacaat cccctgccag cgaaacgtta
     3841 tcttcctgct ccaggtttgc gacaatttac tgcgtcgcaa aaaagcaaac gtttagctcc
     3901 gcgcatacaa aacgggtggt aaaaaaagca aacgattatc cggcgatcat agcggctttt
     3961 tcaaccgaag tttgctgctt ttttctcttt tattgtcttg acgatgaaaa accaggcaat
     4021 tgatgcgcaa acgttatcgt cggtgcgcaa gattgcagcg ttatcgagcc gtataccgtt
     4081 caggattgcg ataaaaaatc gtctgagatg acaaaaatat tgcggataaa aagtatactt
     4141 ttacactggc gaagaaatgc cggtgacata caattaaagc atcaacacca accggaacct
     4201 ccaccacgtg ctcgaatggg gtgtgttgac gtcgggggaa accctcctgt gtaccagcgg
     4261 gatagagaga aagacaaaga ccggaaaaca aactaaagcg cccttgtggc gctttagttt
     4321 tgttcatctt ccagcaagcg tgcgccggta ccttcttctc ctaagcggtc gcccgggtta
     4381 cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca
     4441 cgcagcgcta ctaaggtatg aatgcgccga tccaactctt ctcgccattg ggacgaaagc
     4501 tgtttccact ctttcgcact taacgtatgc ccttcgggca acacgccaaa cgcttcacca
     4561 atggtcgcca gcggaatgcc aatacgctga gcaattttga taattgcaac atatcgcaac
     4621 acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactggtaat caacccttta
     4681 ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac ttcgccgggg
     4741 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
     4801 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
     4861 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
     4921 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
     4981 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
     5041 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
     5101 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
     5161 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
     5221 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
     5281 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
     5341 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
     5401 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
     5461 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
     5521 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
     5581 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
     5641 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
     5701 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     5761 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     5821 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     5881 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     5941 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     6001 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     6061 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     6121 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     6181 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     6241 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     6301 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     6361 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     6421 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     6481 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     6541 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     6601 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     6661 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     6721 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     6781 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     6841 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     6901 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     6961 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     7021 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagt tcacaaaatt
     7081 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     7141 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     7201 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt cttagtgccg
     7261 taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat tatgtcgggc
     7321 ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca atggggttgc
     7381 ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagtcg gtgatgatag
     7441 aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac gtagaagcct
     7501 ccataacgga ggcttctttt ttatggctgg cagatgcttt aatcatccac cttaaaacaa
     7561 tataacctat tgttttaatg acaaatcaga acggaatgtc atcatcaaag tccatcggcg
     7621 gctcgttaga cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga
     7681 actgattgcc accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac
     7741 cgatattacc acctgccgga gcgccaccac cctgacgacc acccagcatc tgcatggtgc
     7801 cgccaacgtt caccacgact tctgtggtgt agcgatcctg gccggattga tcggtccatt
     7861 tgcgggtacg cagctgacct tcgatataaa cctgagaacc tttacgcaga tattcgctgg
     7921 ccacttctgc cagtttgccg aacagcacaa cgcggtgcca ttcagtctgt tctttcatct
     7981 cgccggtcgc tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc
     8041 caccatttgg catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct
     8101 tgtttacgcc tctgctggcc atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt
     8161 aaacgcgcga ttgtaccatt accaatagcg cttttactat gttgtgacct cggttccggg
     8221 aaacaaacct ggccagacat tgttacacaa cactccggat aatgcattcc aatactgtat
     8281 attcattcag gtcaatttgt gtcataatta accgtttgtg atcgccggta gcaccatgcc
     8341 accgggcaaa aaagcgttta atccgggaaa ggtgaatgga taagatcgaa gttcggggcg
     8401 cccgcaccca taatctcaaa aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg
     8461 tgaccgggct ttcgggttct ggcaaatcct cgctcgcttt cgacacctta tatgccgaag
     8521 ggcagcgccg ttacgttgaa tccctttccg cctacgcgcg gcagtttctg tcactgatgg
     8581 aaaagccgga cgtcgatcat attgaggggc tttctcctgc catctcaatt gagcagaaat
     8641 cgacgtctca taacccgcgt tctacggtgg ggacaatcac cgaaatccac gactatttgc
     8701 gtttgttgtt cgcccgcgtc ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg
     8761 cgcaaaccgt cagccagatg gtggataacg tgctgtcgca gccggaaggc aagcgtctga
     8821 tgctactcgc gccaatcatt aaagagcgca aaggcgaaca caccaaaacg ctggagaacc
     8881 tggcaagcca gggttacatc cgtgctcgta ttgatggcga agtctgcgat ctttccgatc
     8941 cgccgaaact ggaactgcaa aagaaacaca ctattgaagt ggtggttgat cgcttcaagg
     9001 tgcgtgacga tcttacccaa cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg
     9061 gtggtacagc ggtagtggcg gatatggacg acccgaaagc ggaagagctg ctgttctccg
     9121 ccaacttcgc ctgcccaatt tgcggctaca gtatgcgtga actggagccg cgactgtttt
     9181 cgtttaacaa cccggcaggt gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt
     9241 tcgatcctga ccgcgtgatc caaaaccccg agctgtcact ggctggcggt gcgatccgtg
     9301 gctgggatcg ccgcaacttc tattacttcc agatgctgaa atcgctggca gatcactata
     9361 agttcgacgt cgaagcgccg tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt
     9421 acggttctgg caaagaaaac attgaattca aatacatgaa cgatcgtggc gatacctcca
     9481 tccgtcgtca tccgttcgaa ggcgtgctgc acaatatgga gcgccgttat aaagagacag
     9541 aatccagtgc ggtacgtgaa gaattagcca agtttatcag caatcgccca tgcgccagct
     9601 gcgaagggac gcgtctgcgt cgggaagcgc gccacgttta tgtcgagaat acgccgctgc
     9661 ctgctatctc cgacatgagc atcggtcatg cgatggaatt cttcaacaat ctcaaactag
     9721 ctggtcagcg ggcgaagatt gcagaaaaaa tccttaaaga gatcggcgat cgtctgaaat
     9781 tcctcgttaa cgtcggcctg aattacctga cactttcccg ctcggcagaa acactttccg
     9841 gcggtgaagc ccagcgtatc cgtctggcga gccagattgg tgcgggcctg gttggcgtta
     9901 tgtatgtact ggacgagccg tctatcggcc tgcaccagcg cgataacgag cgcctgttgg
     9961 gtacgcttat ccatctgcgc gatctcggta ataccgtgat tgtggtggag catgacgaag
    10021 acgcaattcg cgccgctgac catgtgatcg atatcggtcc tggtgcgggt gttcacggcg
    10081 gtgaagtggt cgcggaaggt ccactggaag cgattatggc ggtgccggag tcgttaaccg
    10141 gacagtacat gagcggtaaa cgcaagattg aagtgccgaa gaaacgcgtt ccggcaaatc
    10201 cggaaaaagt gctgaagctg acaggcgcac gcggcaacaa cctgaaggac gtgacgctca
    10261 cgctgccagt cggtctgttt acctgcatca caggggtttc aggttccggt aaatcgacgc
    10321 tgattaacga cacactgttc ccgattgccc aacgccagtt gaatggtgcg accatcgccg
    10381 aaccggcacc gtatcgcgat attcaggggc tggagcattt cgataaagtg atcgatatcg
    10441 accaaagccc aattggtcgt actccacgtt ctaacccggc gacctatacc ggcgtgttta
    10501 cgcctgtgcg cgaactgttt gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag
    10561 gacgtttcag ctttaacgtc cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga
    10621 tcaaagtaga gatgcacttc ctgccggata tctacgtgcc gtgcgatcag tgtaaaggta
    10681 aacgctataa ccgtgaaacg ctggaaatta agtacaaagg caaaaccatc cacgaagtgc
    10741 tggatatgac catcgaagag gcgcgtgagt tctttgatgc cgtacctgca ctggcgcgta
    10801 agctgcaaac gttgatggac gttggcctga cgtacattcg cctggggcag tccgcaacca
    10861 cactttctgg tggtgaagcc cagcgcgtga agctggcgcg tgagctgtca aaacgcggca
    10921 ccgggcagac gctgtatatt ctcgacgagc cgaccaccgg tctgcacttc gccgatattc
    10981 agcaactgct cgacgtactg cataaactgc gcgatcaggg caacaccatt gtggtgattg
    11041 agcacaatct cgacgtgatc aaaaccgctg actggattgt cgacctggga ccagaaggcg
    11101 gcagtggcgg cggcgaaatc ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag
    11161 catcacacac ggcacgcttc ctcaagccga tgctgtaatc gttaaggccg ctttctgagc
    11221 ggccttttcc tttcagagtt gtaccagcaa tttacgcttt tcttccggta gtaaattcac
    11281 tgcctgctga taagacgcat ccaccagata atagatttgc gaatccggca gcgaaccgtc
    11341 gagatacacg gtgctccagt gcgctttatt cagatggcgg ctaggacgca catcgctgtg
    11401 ctgctgacgt agcagctccg ccagctccgg gctggttttc agcgaaacag ctgggcgatt
    11461 ttctacttct ttcaccatcg caaacagtac atcttccact ttgatctgcg tcgctttcca
    11521 gtcgttatgc acgctctgtt ctgcgcctgg ttttgccatg caatattgta gcaactccga
    11581 aatggtcatt ttttactccc cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat
    11641 gcgatgttcc cccagccaga tgccttgcca ggtgccggtc tgaatacgcc ctttatgtac
    11701 cggcaataca agcgatgttc ccagcattga ggatttgata tgagaaggca tatcgtctgc
    11761 tccctcatag tcatgctcat aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc
    11821 catgtcgtgg cgtacggtgg gatcgcagtt ctcattaagt gtcagagagg cggaggtatg
    11881 ttgcagcaac agatgcagta agccgatgtt aacgcgcggc atatcagcca gctgattcag
    11941 aatttcatcc gttaccagat gaaacccacg agatttggcg ctaagcgtga gcgtcttttg
    12001 ataccacatg tgctgctcct tgataaaact ctcttaatca gtttgcagca agacagcgaa
    12061 aggataaagg tgtgattaaa aaaacagcat tcgggagagc gtcacgctct cccgctctgt
    12121 cagtattctg aattgacgat cacctcttca ccaaacgcac ccgcttgtgg caagggtttg
    12181 taggtagagt tggaggcgcg cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg
    12241 gtaatatcat tatcagaatc gccataaaaa attcggatat ttttatcctg cagccattgc
    12301 gattttgtat tttgccctgg tttatcgccc gcaaagatca ccggattcat gttggtggca
    12361 ggaatatgaa aattatccgc cagcgttttt gaaaccgttt ctgttttcgt cggactacga
    12421 ccagtcacaa agaagatcgc gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg
    12481 acctcttttg gaatgctgaa ttcatcccag ccattgttca ttttttccca gaacacagga
    12541 tttttcagat aatcttcgct ttctggcgag aaggtttttt tgccgcgcca gaagcccgga
    12601 ctggaaaaaa gtaccgtgtc atcgatatca aaccccaccg ccattggcgg acgccctgcg
    12661 aggctatttt caatttgtgc gaccgaaacc caatgaatgg gtgcctgttc agcaagcctg
    12721 gcaacgttag tcccagggtt aagcggtgaa ggagatgagg ccagggcaac agcggaactg
    12781 tttagagcaa acaataagca aacggcactg attgcctgtg tgatcttgcg catatttttc
    12841 cctaaatagt cagtatgttg aaacttttta ttgtgagatt tgttgcaaaa accatctgac
    12901 cataacgcca gctgccgggg aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg
    12961 atgattatat ttaacccagt aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt
    13021 actcgatgta aggatacatt aattttacgt ttattatgat gaatcctcgg caaatgagcg
    13081 aaatttgata gaacatttat ctgctgtacg atttaatgaa cttaatgata cagcattaca
    13141 aaaacaatcg aagtttataa agatgatttc tgattgacca gccccctacc ctacaa
//
LOCUS       DAZGCS010000123        13015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGCS010000123 DAZGCS010000000
VERSION     DAZGCS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.125"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            345..926
                     /gene="mdaB"
                     /locus_tag="ACHK01_004096"
     CDS             345..926
                     /gene="mdaB"
                     /locus_tag="ACHK01_004096"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO8098961.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            957..1271
                     /locus_tag="ACHK01_004097"
     CDS             957..1271
                     /locus_tag="ACHK01_004097"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO8098962.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1319..3211)
                     /gene="parE"
                     /locus_tag="ACHK01_004098"
     CDS             complement(1319..3211)
                     /gene="parE"
                     /locus_tag="ACHK01_004098"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO8098963.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDRDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3240..3821)
                     /gene="yqiA"
                     /locus_tag="ACHK01_004099"
     CDS             complement(3240..3821)
                     /gene="yqiA"
                     /locus_tag="ACHK01_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO8098964.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3821..4648)
                     /gene="cpdA"
                     /locus_tag="ACHK01_004100"
     CDS             complement(3821..4648)
                     /gene="cpdA"
                     /locus_tag="ACHK01_004100"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO8098965.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4673..5095)
                     /locus_tag="ACHK01_004101"
     CDS             complement(4673..5095)
                     /locus_tag="ACHK01_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO8098966.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5096..5725)
                     /gene="nudF"
                     /locus_tag="ACHK01_004102"
     CDS             complement(5096..5725)
                     /gene="nudF"
                     /locus_tag="ACHK01_004102"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO8098967.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5930..7411
                     /gene="tolC"
                     /locus_tag="ACHK01_004103"
     CDS             5930..7411
                     /gene="tolC"
                     /locus_tag="ACHK01_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO8098968.1"
                     /translation="MKKLLPILIGLSLYGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7559..8230
                     /locus_tag="ACHK01_004104"
     CDS             7559..8230
                     /locus_tag="ACHK01_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO8098969.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8236..9396
                     /locus_tag="ACHK01_004105"
     CDS             8236..9396
                     /locus_tag="ACHK01_004105"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8098970.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQIEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9434..10222)
                     /gene="ygiD"
                     /locus_tag="ACHK01_004106"
     CDS             complement(9434..10222)
                     /gene="ygiD"
                     /locus_tag="ACHK01_004106"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO8098971.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10365..11138
                     /gene="zupT"
                     /locus_tag="ACHK01_004107"
     CDS             10365..11138
                     /gene="zupT"
                     /locus_tag="ACHK01_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO8098972.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11196..11366)
                     /gene="yqiD"
                     /locus_tag="ACHK01_004108"
     CDS             complement(11196..11366)
                     /gene="yqiD"
                     /locus_tag="ACHK01_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO8098973.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11627..12280)
                     /gene="ribB"
                     /locus_tag="ACHK01_004109"
     CDS             complement(11627..12280)
                     /gene="ribB"
                     /locus_tag="ACHK01_004109"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO8098974.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12384..12532)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12654..12944
                     /gene="ubiK"
                     /locus_tag="ACHK01_004110"
     CDS             12654..12944
                     /gene="ubiK"
                     /locus_tag="ACHK01_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO8098975.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3293 a         3472 c         3171 g         3079 t
ORIGIN      
        1 attgtttgaa ttttatgctt taacatatat cagtacgctc ataaaaatcc atttataatt
       61 atcaacatga cacgttgata ttttcatctt ttacttattc aaagccatat tttcaatccc
      121 aaaaaaaacc gaaccaaacg tgtaaattat tccggtgcga gtaagaatta tgaggaatgg
      181 ctatcagtat tgtcattttc agaaaatatt tatcctgcat cggtgagtca gagcaagatc
      241 agacttttgc taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct
      301 gtgacgtact atagtcggca aacgtctcac cttgaggtta aaaaatgagc aacatcctga
      361 ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg
      421 aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca
      481 gcgactacga tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc
      541 agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat
      601 tcaccgaagg tcatggaacg ctgtatgcca gcgatggccg tacccgcaaa gatccgtcaa
      661 aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga
      721 acgcaccaat ggaagccttc accgaaaaag atcaattctt ccacggcgtt ggcgtcgacg
      781 gtgtgtatct gccgttccat aaagcaaacc aattccttgg catggaaccg ctgccgacat
      841 ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca
      901 agcatcttgt ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc
      961 ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg
     1021 atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc
     1081 caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga
     1141 tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga
     1201 aggcgtacag cgaagccgta aaaggcgacg tgctggagat gaatatccgc atcctgcaac
     1261 ctgggattta aacctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt
     1321 aaacctcaat ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc
     1381 gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga
     1441 tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg
     1501 ggttcatttc ccccagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta
     1561 attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga
     1621 gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc
     1681 ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca tcagagtccg
     1741 catccgcgag gatacagatt ttgccataac gaagctggct cagatcgtcg cggtcaggat
     1801 cgataccgat cgctaccgaa atatcatgca cttcctgcga ggccagcact tcgtcggaag
     1861 aaacttccca ggtgttaagg atcttacctt tcagtggcat gatcgcctga tattcgcgat
     1921 cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacaaggaac agttcggtac
     1981 ggttaagatc ctgcgcggta cagtcagcca gtttgccagg cagtgctggg ccgctggtca
     2041 gctttttgcg caccactttt ttagccgcac gcatacggcg ctgggcgctg gaaatcgcca
     2101 tctccgccag cagctccgcc gcctgaacgt tctggttcag ccacaggata aaggcatctt
     2161 tcaccacgcc tgaaacgaat gccgcgcatt gacgcgaaga gagacgctct ttggtctgcc
     2221 cggcaaactg cggatcctgc attttcaccg acagaacata ggcgcagcga tcccagatat
     2281 cttccgccga cagcttcacg ccgcgcggca gaatgttgcg gtattcacag aactcacgca
     2341 tcgcgtccaa caggccctga cgcagaccat taacatgggt accgccctgc atcgttggga
     2401 taaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc
     2461 agtccacagc ttcagtatcg ccagcgaaat taccgataaa cggtttttct ggcagcgtcg
     2521 gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct
     2581 gttcggtgtt gttgatctca tctttaaagg taatctcaac gccggggcac agtactgctt
     2641 tggccttcag cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt
     2701 catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg ccgacaacct
     2761 gtaaatcctg caccttttcg ccattttcaa aggcgatgtt ataaacctga ccatcgcggc
     2821 gcacgttaac ttctacgcgc ttcgacaggg cgttaaccac cgaaatcccc acgccatgca
     2881 ggccgccaga gaactggtaa tttttgttag agaatttacc gcctgcatgc agacggcaaa
     2941 gaatcagttc aaccgccggt acaccctctt ccgggtgaat atccaccggc atcccgcgcc
     3001 catcgtcaat aacttctaac gactggtcag catgtaagat tacatccacg cgttttgcgt
     3061 gacccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa tggttagggc
     3121 gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct
     3181 caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg agttgatcgt
     3241 cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg
     3301 aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcgtagta cgccaccgcc
     3361 tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct
     3421 tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc tagcacatat
     3481 tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg
     3541 cgcaccgccg ggtttaccac cactgcgggc agcataaaac attgtgacaa ccaggtggcg
     3601 taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca
     3661 atggattcca gcagctctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc
     3721 tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc
     3781 ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg
     3841 tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag
     3901 catgtaactc gagagtacgc cagccgggcg cgatagtatc cagcgtaaag ttggaacagt
     3961 gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga
     4021 gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca
     4081 gcacggtatc cagttcgccc gcgttacgca gactgtgttg atcgagccaa ctacaacccg
     4141 caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca tcggccagtt
     4201 tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct
     4261 ggctatccag caacaggatt tgccattgct caccaataaa cacgcgcttc gccggggaga
     4321 tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg cccggcagcc
     4381 agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag gccgcagagg
     4441 attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tgatgtggac
     4501 gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac agggcttcgt
     4561 gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat cctgactctg gcctcaccag
     4621 ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa
     4681 ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt
     4741 taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt
     4801 tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac
     4861 gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca
     4921 ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg tttgccacct
     4981 gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa
     5041 agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg
     5101 cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg
     5161 ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat
     5221 gaacgcgaat atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt
     5281 ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac
     5341 ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg
     5401 caacatcttc cacactttca ccctcttcta tcatcccggc aaccatctcc agtagccagg
     5461 gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc aatcagcaca acttcatcac
     5521 gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tccctccgta
     5581 cctcatgact catttgcccg ttgaataaac gatgacgaaa tctataaaga tctaatgaaa
     5641 aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa
     5701 cgggcaggtt gtctggctta agcattgtta atgtcctggc actaatagtg aattaaatgt
     5761 gaatttcagc gacgtttgac tgccgtttga gcagtcatgt gttaaattga ggcacattaa
     5821 cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga atccgcaata
     5881 attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt
     5941 gctccccatt cttatcggcc tgagccttta tgggttcagt tcgttgagcc aggccgagaa
     6001 cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc
     6061 cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct
     6121 aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc
     6181 taacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg
     6241 tgcgttaacg ctgcaggaaa aagctgcagg gattcaggac gtcacatatc agaccgatca
     6301 gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt
     6361 tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca
     6421 acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga
     6481 taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg tagagcagct
     6541 gcgccagatc accggtaact actatccaga actggcagcg ctgaatgtcg aaaactttaa
     6601 aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc
     6661 gctgttacag gcacgcttga gccaggacct ggcgcgcgag caaattcgcc aggcgcagga
     6721 tggtcactta ccgacgctgg atttaacggc ttctaccggg atttctgaca cctcttatag
     6781 cggttcgaaa actcgtggtg ccgctggtac ccagtatgac gacagcaata tgggccagaa
     6841 caaagtgggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt
     6901 gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa ctggaaagcg cgcatcgtag
     6961 cgtcgtgcaa accgtacgtt cctccttcaa caacattaat gcatctatca gtagcattaa
     7021 cgcctacaaa caagccgtag tttccgctca aagctcatta gacgcgatgg aagcgggcta
     7081 ctcggtcggt acgcgtacca ttgttgatgt gttggatgca accaccacgc tgtacaacgc
     7141 taagcaagag ctggcaaatg cgcgttataa ctacctgatt aatcagctga atattaagtc
     7201 agccctgggt acgttgaacg agcaggatct gctggcactg aacaatgcgc tgagcaaacc
     7261 ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg ctattgctga
     7321 tggttatgcg cctgatagcc cggcacccgt cgttcagcaa acatccgcac gcactaccac
     7381 cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg
     7441 aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa
     7501 tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat
     7561 gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg cgcgccatct
     7621 gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg aaaagagtga
     7681 tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag
     7741 cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa
     7801 atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc
     7861 agcccaggct ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt
     7921 ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt
     7981 tgcacagcag ccgctgttct cctcgaaaaa cccagccagc ccggcttacg gtaaatatac
     8041 cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa
     8101 gacagccatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg gttttggtga
     8161 atctgttgcc aaacaaagca ctatgcagcg tagcgcaact ggcacctctt ctcgttcaat
     8221 gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgagaaa
     8281 gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat
     8341 gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg
     8401 caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa
     8461 ttccgcattc caaaacacac ctggagtttt gtgcgccagt catggctgac gcaccagcca
     8521 tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa acttctggaa
     8581 aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg
     8641 gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa
     8701 gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca gttgctgcat
     8761 ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc
     8821 gcaacggaag ctgaaattgc tactgagttc ctctacatcg atgatatcgg gttaggtgaa
     8881 aaaggtcagt tcacggattt acaggatcag gtaatttcca acctgttcaa gctgtatccg
     8941 tgggaattta tgttgcgtga gatgttttcc accaagctgg aggatgcagg cgtacgctgg
     9001 ctggaaccgg cgtggaagag cattatctcc aacaaggcac ttctaccgct actgtgggag
     9061 atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa
     9121 atagaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt
     9181 gaaaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt
     9241 cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg
     9301 gtgaacgatc aacccgccgg aattggcatt cgtgaagacc gtgcattgat cacccaggat
     9361 atgtctcggt tttatccaca tatttttgtt gagtaatcaa tgatgccgga tggcattcgc
     9421 catccggtaa ttgttaacct atctgcaccg acagcatact caggctgccc atttctatac
     9481 catcgaccgg aatggtaatt ggctcctgcc catcccacgc acctaagaca tacaacaacg
     9541 gcagatagtg ctccggcgtt gggttcgata acgcgccacc ttcatggtcg aggtaattca
     9601 ccagaggatg ctgctctact gccccttgcc acgtcagatt cgctttcaca tactcattaa
     9661 acgacatcgc ccaaggatat ggtgaactat caccgtgcca cttcactgta cgcaggttat
     9721 gcaccacgtt gccactggcg accaacatta ttccttcatc tcgcagcgct gccagtttgc
     9781 gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatcg
     9841 ggatatcggc gtcaggatac atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc
     9901 aggcttcttt atccagcgtc accgggaccg gcgctaacag ctcaaccaga tgctgtgcca
     9961 gcacaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa
    10021 agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt
    10081 gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc
    10141 tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg
    10201 ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt ttgatatcct
    10261 cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta
    10321 ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca
    10381 ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcgggc
    10441 aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt tgcggcgggg atcatgttgc
    10501 tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt
    10561 tgggttatgg aatgtttatc ttcggtctgc ttggctattt tggcctggac cgcatgttgc
    10621 cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca
    10681 agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg
    10741 ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg
    10801 ctttgcacaa tatccctgaa ggtctggcag tggcaggccc ggtttatgcg gcaacggggt
    10861 ctaaacgcac cgcgattctg tgggcgggga tttctggact agcagaaatt cttggtggtg
    10921 tgctggcgtg gttaatcctc ggtagcatga tttccccggt ggtcatggcg gcaatcatgg
    10981 cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg ctcgccaaag
    11041 agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca gtgatgggat
    11101 tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag aagttgtcca
    11161 gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc ggctgtctct
    11221 tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct gtcctggcga
    11281 aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag agcaatcaat
    11341 acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa tactaaacat
    11401 ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg
    11461 aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc
    11521 atagaaagag aagcggaatt aacatcttgc atagcggggc aaaataaaaa ccgaagccag
    11581 ctacaacttc ggtttcttga ttaaggcagt aattaagcag cggttttcag ctggctttac
    11641 gctcatgtgc ctgacggtat gccaccaggt cttcaatagt cacgagcgcc atattgtgtt
    11701 tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca ttagtcagct
    11761 cacacagcac accagccggt ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag
    11821 tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg
    11881 ggcgattcag atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac
    11941 gatcagcggc agaaacaccg gtagtcacac cttcagccgc ttcaatggtc acggtaaaac
    12001 cggtgccata ggcgctggtg ttattttcta ccatcattgg cagatcaagt tgtttacggc
    12061 gatcttcagt gatgcacagg caaacaatac cgctaccgtg gcgaatggtc agcgccatct
    12121 gctcaacggt catggtttct gccgggaaga tcatatcgcc ttcgttttca cggtcttcat
    12181 catcaagcac cattacaccg cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt
    12241 cgaaaggcgt accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa
    12301 attatggtta ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg
    12361 agcgggtcca tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc
    12421 cggaattaca ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca
    12481 actgagttga tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac
    12541 tataacgcta atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt
    12601 acccttatcg cactacaatg gtactcaaca cctatcagta cagggaaact acaatgattg
    12661 acccgaaaaa aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca
    12721 gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc
    12781 gcctcgacct tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc
    12841 gtgagaaact ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga
    12901 ttaaaaagca gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg
    12961 cactgtaaat tatgaattaa tcctctaatt tacagtgctt tctattacct tatcg
//
LOCUS       DAZGCS010000124        13001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGCS010000124 DAZGCS010000000
VERSION     DAZGCS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.126"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            206..1315
                     /gene="livK"
                     /locus_tag="ACHK01_004111"
     CDS             206..1315
                     /gene="livK"
                     /locus_tag="ACHK01_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO8098976.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1363..2289
                     /gene="livH"
                     /locus_tag="ACHK01_004112"
     CDS             1363..2289
                     /gene="livH"
                     /locus_tag="ACHK01_004112"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO8098977.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2286..3563
                     /gene="livM"
                     /locus_tag="ACHK01_004113"
     CDS             2286..3563
                     /gene="livM"
                     /locus_tag="ACHK01_004113"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO8098978.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3560..4327
                     /gene="livG"
                     /locus_tag="ACHK01_004114"
     CDS             3560..4327
                     /gene="livG"
                     /locus_tag="ACHK01_004114"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO8098979.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4329..5042
                     /gene="livF"
                     /locus_tag="ACHK01_004115"
     CDS             4329..5042
                     /gene="livF"
                     /locus_tag="ACHK01_004115"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO8098980.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5438..6754
                     /gene="ugpB"
                     /locus_tag="ACHK01_004116"
     CDS             5438..6754
                     /gene="ugpB"
                     /locus_tag="ACHK01_004116"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO8098981.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6852..7739
                     /gene="ugpA"
                     /locus_tag="ACHK01_004117"
     CDS             6852..7739
                     /gene="ugpA"
                     /locus_tag="ACHK01_004117"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO8098982.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7736..8581
                     /gene="ugpE"
                     /locus_tag="ACHK01_004118"
     CDS             7736..8581
                     /gene="ugpE"
                     /locus_tag="ACHK01_004118"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO8098983.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8583..9653
                     /gene="ugpC"
                     /locus_tag="ACHK01_004119"
     CDS             8583..9653
                     /gene="ugpC"
                     /locus_tag="ACHK01_004119"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO8098984.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKCRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9650..10393
                     /gene="ugpQ"
                     /locus_tag="ACHK01_004120"
     CDS             9650..10393
                     /gene="ugpQ"
                     /locus_tag="ACHK01_004120"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8098985.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10380..10820)
                     /locus_tag="ACHK01_004121"
     CDS             complement(10380..10820)
                     /locus_tag="ACHK01_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO8098986.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10940..12682
                     /gene="ggt"
                     /locus_tag="ACHK01_004122"
     CDS             10940..12682
                     /gene="ggt"
                     /locus_tag="ACHK01_004122"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO8098987.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAINFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12720..>13001)
                     /locus_tag="ACHK01_004123"
     CDS             complement(12720..>13001)
                     /locus_tag="ACHK01_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO8098988.1"
                     /translation="ITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLE
                     TGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2874 a         3332 c         3736 g         3059 t
ORIGIN      
        1 ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga ctgaaaaata
       61 gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag acaggcaaaa
      121 acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca
      181 acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat
      241 gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagttg ccgttgtcgg
      301 cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc
      361 gattaaagac attaatgcca aagggggaat taaaggcgat aagctggtgg gcgtggaata
      421 tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaacgacgg
      481 cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcgt cagatatcta
      541 cgaagacgaa ggtattctga tgatctcgcc gggagcgacc aacccggagc tgacccaacg
      601 cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc ctacggcggc
      661 aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca
      721 gtatggcgaa gggctggcgc gttcggtgca ggacgggcta aaagcggcta acgccaacgt
      781 cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga tcgcccgcct
      841 gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat
      901 gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg aaggtgtggg
      961 taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg tcactatgcc
     1021 aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga aagcagacaa
     1081 gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat ctctggcgac
     1141 tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa
     1201 cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt
     1261 tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca agtgatcatc
     1321 ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga gcagtttttg
     1381 tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgatagcc
     1441 atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca cggcgaggtt
     1501 tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat gatgggcatt
     1561 gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc
     1621 tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa gcgcctgatt
     1681 gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag cctgaccgaa
     1741 ggttcgcgtg acgtggcgct gcccagtctg tttaacgggc agtgggtggt gggccatagc
     1801 gaaaacttct ctgcctctat tactaccatg caggcggtga tttggattgt taccttcctc
     1861 gccatgctgg cgctgacgat tttcattcgc tattcccgta tggggcgcgc ctgtcgtgcc
     1921 tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg ggtgattgcg
     1981 ctgacctttg tgattggcgc ggcgatggcg gcggtggcgg gtgtgctgct cggtcagttc
     2041 tacggtgtga ttaaccccta catcggcttt atggccggga tgaaagcctt taccgcggcg
     2101 gtgctcggcg ggattggcag cattccgggg gcgatgattg gcgggctgat cctggggatt
     2161 gcggaggcgc tctcttctgc ctatctgagt acggaatata aagatgtggt ctcatttgcc
     2221 ctgctgatcc tggtactgct ggtgatgccg accggtattc tggggcgccc ggaggtagag
     2281 aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg ttctttatcc
     2341 tcgctggcgt ctttatgggc gtacagctgg agctggacgg caccaaactg gtggtcgaca
     2401 cggcttcgga tgtccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc
     2461 agattttgcg acctgctttc cagaaagggt tgaaaagcgt ttccgggccg aagtttattc
     2521 tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg ctgttggtgc
     2581 ttgcggtggc gtggccgttt atggtttcac gcgggacggt ggatatcgcc accctgacca
     2641 tgatctacat tatcctcggc ctcgggctga acgtggtggt agggctttcc ggcctgctgg
     2701 tgctggggta cggcggtttt tacgccatcg gcgcttacac ttttgcgctg ctcaatcact
     2761 attacggctt gggcttctgg acctgtctgc cgattgccgg gttaatggca gcggcggcgg
     2821 gcttcctgct cggctttccg gtgctgcgtc tgcgtggtga ttatctggcg atcgttaccc
     2881 tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggcc
     2941 cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc agccgtaccg
     3001 cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac gatccctccg
     3061 atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc ctgtttgtca
     3121 ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt gaagatgaga
     3181 tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct tttaccatca
     3241 gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc tttgtcagcc
     3301 cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg ctcggcggta
     3361 tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg cgcgagttga
     3421 tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga
     3481 tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg aaaaacggcg
     3541 cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc tgatgatgcg
     3601 cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt
     3661 ctcgttaatc ggccctaacg gtgccggaaa aaccacggtt tttaactgcc tgaccggttt
     3721 ctacaaaccc accggcggca ccattttact gcgcgatcag catcttgaag gtttaccggg
     3781 gcagcaaatt gcccgcatgg gcgtggtgcg caccttccag catgtgcgtc tgttccgtga
     3841 aatgacggta attgaaaacc tgctggtggc gcagcatcag caactgaaaa ccgggctgtt
     3901 ctctggcctg ttgaaaacgc cttctttccg tcgcgcccag agcgaagcgc tcgaccgcgc
     3961 cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct
     4021 ggcctatggt gaccaacgcc gtctggagat tgcccgctgc atggtgacgc agccggagat
     4081 tttaatgctc gacgaaccgg cggcaggtct taacccaaaa gaaaccaaag agctggatga
     4141 gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg aacacgatat
     4201 gaagctggtg atggggattt cagaccgaat ttatgtggtc aatcagggga cgccactggc
     4261 aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct atttaggtga
     4321 ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa
     4381 tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgagattgtc accctcattg
     4441 gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat ccgcgcgcta
     4501 ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca
     4561 tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg
     4621 aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag gagcgcataa
     4681 agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg gcaggcacca
     4741 tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc aacccgcgtt
     4801 tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag caaattttcg
     4861 acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag cagaacgcca
     4921 accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat gtggtgctct
     4981 ccgataccgg cgacgcgctg ctggcgaacg aagcagtgag aagtgcgtat ttaggcgggt
     5041 aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga caaatgtaaa
     5101 attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata ttgaatttgc
     5161 acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg atgagtaatc
     5221 tgtagagttt gattcagacc ttctattttc cgcttatccg tgccccatct cccattttcc
     5281 ctcacccacg ccgtcaccgc cttgtcatct ttctgacacc ttactatctt acaaatgtaa
     5341 caaaaaagtt atttttctgt aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg
     5401 tgaattcgcg cattcggtac aacaagagag ataaacgatg aaaccgttac attatacagc
     5461 ttcagcactg gcgctcggac tggcgttaat ggggaatgca caggcagtga cgaccattcc
     5521 gttctggcat tctatggaag gggaactggg taaagaggtg gattctctgg cccaacgttt
     5581 taacgccgaa aatccggatt acaaaattgt accgacctat aaaggcaact acgaacagaa
     5641 tttaagcgcg gggattgccg catttcgtac cggcaacgct ccggctattt tgcaggttta
     5701 tgaagttggc accgccacca tgatggcatc gaaagccatt aaaccggtat atgacgtgtt
     5761 taaagaggcg gggattcaat tcgatgagtc gcagtttgtg ccgacggttt ccggttacta
     5821 ctccgacagc aaaacgggcc acttactctc ccagccattc aacagctcga cccccgttct
     5881 ctattacaac aaagacgcct tcaagaaagc aggattagac ccggaacaac cgccgaaaac
     5941 ctggcaggat ctggcggact atgccgcgaa actgaaagcc tccggtatga agtgcggcta
     6001 cgccagcggc tggcagggct ggatccaact ggaaaacttt agcgcctgga acggtctgcc
     6061 gtttgccagc aaaaacaacg gctttgacgg cacggacgcg gtgctggagt tcaacaagcc
     6121 ggagcaggtg aaacacatcg ccatgctcga ggagatgaac aagaagggcg acttcagcta
     6181 cgtcggtcgt aaggatgaat ccaccgagaa gttctataac ggtgattgcg cgatgaccac
     6241 cgcctcttcc ggttctctcg ctaatattcg tgagtacgcc aaatttaact atggcgtagg
     6301 catgatgcct tacgacgccg atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc
     6361 cagcctgtgg gtgatgcagg gcaaaaataa agaaacgtat accggcgtgg cgaagttcct
     6421 cgacttcctc gcgaagccag aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc
     6481 aatcaccaaa gcagcgtatg acctgacccg tgagcagggc ttttacgaga aaaacccagg
     6541 ggcggatacc gcgacgcgtc agatgctgaa taagccgccg ttgccgttca ccaaagggct
     6601 gcgtctgggc aacatgccgc agatccgcgt gattgtggat gaagagctgg agagcgtgtg
     6661 gaccggtaag aagacaccac agcaggcact ggataccgcc gttgagcgtg gaaatcagtt
     6721 gctgcgccgc tttgagaaat cgacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc
     6781 ttatctgacc tggcatcgcg tgtaggccgg ataagcgaag cgcatccggc acagttcagg
     6841 aattaaccgt aatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccttatctgc
     6901 tggtcgcgcc gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt
     6961 tgtggtactc gctacaaagc gtcgatccgt ttggtttctc cagccagttt gtcggcctgg
     7021 ataacttcgt cacgctgttc catgacagct actatctcga ctccttctgg acgacgataa
     7081 aattcagcac ctttgtcacc gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg
     7141 tggagtacat cgtgcgcggc agccgtttct atcaaacctt aatgttgctg ccttacgccg
     7201 tggctcccgc cgttgccgcc gtattgtgga tcttcctgtt taaccccggt cgcgggctga
     7261 tcacccattt tctcgcggag ttcggctacg actggaacca cgcgcaaaac agcggtcagg
     7321 caatgtttct ggtggtgttt gcctcagtat ggaagcaaat cagctacaac ttcctgttct
     7381 tctatgccgc gctgcaatcc attccccgtt cgttgatcga agccgcagcc atcgacggtg
     7441 cagggccgat tcgccgcttc tttaagattg cgctgccgct tatcgccccg gtgagtttct
     7501 tcctgctggt agtgaacctg gtgtatgcct tcttcgacac cttcccggtg atcgacgccg
     7561 ccacgtccgg cgggccggtt caggccacca cgacgctgat ttataagatc taccgcgaag
     7621 gttttaccgg actggatctg gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg
     7681 tcatcgtgct gacggtggtg cagttccgct atgttgaaag caaggtgcgt taccaatgat
     7741 tgagaaccgt ccgtggctga cgatattcag ccataccatg ctgatcctcg ggatcgcggt
     7801 gatcctcttc ccgctgtacg tggcgtttgt cgcggcgacg ctggataaac aggccgtcta
     7861 tgccgcgccg atgacgctca tccccggcac acatctgctg gaaaacatcc acaacatctg
     7921 ggtgaacggg gtaggcacga atagcgcgcc gttctggcgg atgttgctta acagctttgt
     7981 gatggcgttc agcattacgc tcggcaaaat taccgtctcg atgctctcgg catttgccat
     8041 tgtctggttt cgttttccgc tacgtaacct cttcttctgg atgattttta tcaccctgat
     8101 gctgccggtt gaagtacgta tcttcccgac ggtggaagtc atcgccaacc tgcagatgct
     8161 cgacagctac gccggtttaa cgctgccgct gatggcctcg gcgaccgcta ctttcctgtt
     8221 ccgccagttc tttatgacgc tgccggatga gctggtggaa gccgcgcgga tcgacggcgc
     8281 atcgccaatg cgcttctttt gcgacatcgt ttttccgctc tccaaaacta atctggcggc
     8341 gctgtttgtg atcaccttta tctacggctg gaatcagtat ttgtggccgt tgttgattat
     8401 taccgatgtg gatctcggca ccaccgtggc agggatcaaa gggatgatcg ctacaggcga
     8461 aggcaccacg gaatggaact cagtgatggc ggcgatgttg ttaacgctta tccctccggt
     8521 ggtgattgtt ttagtgatgc agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata
     8581 agatggcagg actgaaatta caggcagtaa ccaaaagctg ggatggtaaa acccaggtca
     8641 ttaaaccgct gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtctg
     8701 gctgcgggaa atcgacgctg ctgcgcatgg ttgccgggct ggagcgggtg acagaaggcg
     8761 atatctggat caacgaccag cgcgtgactg aaatggagcc aaaagatcgc gggattgcga
     8821 tggtgttcca gaactacgcg ctttatccgc atatgagtgt cgaagaaaac atggcgtggg
     8881 ggctgaaaat tcgcggcatg ggcaagcagc aaattgccga gcgcgttaaa gaagcggcgc
     8941 gcattctgga gctggacggt ctgctcaaat gtcgcccgcg cgagctttcc ggcggtcagc
     9001 gccagcgtgt ggcgatgggc cgcgcgattg tgcgcgatcc ggcggtgttc ctgtttgatg
     9061 agccgctctc taacctcgat gccaagctgc gcgtgcagat gcgtcttgaa ctgcaacagt
     9121 tgcaccgtcg cctgaaaacg acttcactct acgttactca cgatcaggtt gaagcgatga
     9181 cgctcgccca gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag
     9241 ttgaagtcta cgaaaagccc gccagcctgt ttgtagcgag ttttatcggc agtccggcga
     9301 tgaacctgct gacaggccgc gtgaataccg aaggcacgca tttcgaactg gacggcggta
     9361 tcgtgctacc gctaaacggt ggctaccgtc agtatgccgg gcgtaaaatg actctcggca
     9421 ttcgcccgga acatattgcg ctaagctcgc aagcagaagg tggcgtaccg ctggtgatgg
     9481 atacgctgga gatcctcggc gcagataacc tggcgcacgg acgctggggc gagcagaagc
     9541 tggtggtgcg actggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc
     9601 tggcggaaaa tcagctgcat ctttttgatg gtgaaacagg acaacgagta tgagtaactg
     9661 gccttatccc cgcatcgtcg ctcatcgtgg cggcggtaag ctggcccccg aaaacaccct
     9721 ggcggcaatc gacgttgggg caaaatacgg tcataagatg atcgaatttg acgcgaagtt
     9781 atcgaaagat ggcgagatct tcctgctcca tgacgacaat ctcgaacgca ccagcaacgg
     9841 ctggggcgtc gcgggtgaac tgaactggca ggatttactg cgcgtggatg cgggcagttg
     9901 gtacagcaaa gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg
     9961 tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc accactggca ctggaccatt
    10021 aacgggcaaa atggtggcgc tggcggcacg ccaactgtgg gccggtatga cgccgccgct
    10081 gctgtcatcg tttgagattg atgctttaga agctgcacag ctggcggcac cggaactgcc
    10141 gcgtggttta ttgcttgatg agtggcgcga cgactggcgc gaactgaccg cgcggctggg
    10201 ctgcgtctct attcatctca atcataagtt actcgataaa gtgcgagtga tgcagttgaa
    10261 agacgccgga ctgcggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt
    10321 gctgcgctgg ggcgtggatt gcatctgtac cgatgcgatt gacgtgattg gtccgaactt
    10381 tacggcccaa tagttttcaa cggaatgtca ggctgcggcg tgctgggctg attcagcatg
    10441 tcgccgtttc tcctctccgg cagcatatgc tgctgattca acgaactatc cggattacgg
    10501 ttgctgttta acatcccgcc gttggtgttg ggcagcattt gctgccgggc gggatttcgc
    10561 tcccccggct gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc
    10621 tgctgttgca actgagtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt
    10681 tgctgagtct gcatctgggt ttgcatccgc tgctggctgg gtatctgata ccccggctgg
    10741 ttagggttgt tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta
    10801 agaatcagaa gtcgtttcat cgcgtatcct cctctgaaga tatcctttaa gtttactcgc
    10861 ttcccgacaa aacgatgatt aattcagagt tatataccag gcttagctgg ggttgcccct
    10921 taatctctgg agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc
    10981 tctgctctca ggaagttgtt ttagcgccgc cgccgcgcct cctgcgccgc ccgtctcgta
    11041 tggtgtggag gaagatgtct tccacccggt acgcgcgaaa cagggaatgg tagcgtctgt
    11101 ggacgccact gccactcagg tgggggtgga tattctcaag gagggcggga atgccgttga
    11161 tgccgccgtg gcggtgggct acgcgctggc ggtaacgcat ccgcaggcag ggaatctggg
    11221 cggtggtggt tttatgttaa tccgctcgaa aaatggcaat accacggcta tcaatttccg
    11281 cgaaatggca cccgccaaag cgacccgcga tatgttcctc gatgatcagg gcaacccgga
    11341 cagcaaaaaa tcactcactt cgcatctggc ttccggcaca ccgggtacgg tagcaggttt
    11401 ctcgctggcg ctggataaat acggcaccat gccgctgaac aaagtcgtgc agcccgcgtt
    11461 taaactggca cgcgatggtt ttatcgttaa cgacgcgctg gctgacgatc tcaaaaccta
    11521 cggtagcgaa gtgttgccga atcacgaaaa cagtaaagct atcttctgga aagagggcga
    11581 gccgctgaaa aagggcgaca cgctggtgca ggcgaacctg gcaaagagcc tggagatgat
    11641 tgctgaaaac ggcccggacg aattctataa aggcacgatt gcggaacaga tcgcccagga
    11701 gatgcagaaa aacggtggct tgatcactaa agaagattta gcggcctata aagcggtcga
    11761 acgcactccg ataagcggcg attatcgcgg gtatcaggtt tactccatgc caccgccatc
    11821 ctccggcggg atccatatcg tacaaatcct caatattctg gaaaacttcg atatgaagaa
    11881 atacggcttt ggcagcgccg atgcgatgca aatcatggca gaggctgaga aatatgccta
    11941 cgccgaccgc tcggaatatc ttggcgaccc ggattttgtc aaagtcccgt ggcaggcgct
    12001 gaccaataaa gcctatgcca aatctattgc cgagcaaatc gatatcaata aagcgaaacc
    12061 gtccagcgag attcgccctg gcaagcttgc gccttatgag agtaatcaaa ctacccatta
    12121 ctcagtggtg gataaagatg gtaacgcggt ggcggtgacc tatacgctga acaccacctt
    12181 cggtacgggt attgtcgcgg gcgagagcgg tattctgctt aataaccaga tggatgattt
    12241 ctccgccaaa ccgggcgtac cgaacgttta cgggctggtg ggcggtgatg ccaacgcggt
    12301 cgggccgaat aaacgcccgc tgtcgtcgat gtcaccaacc attgtggtga aagacggtaa
    12361 aacctggctg gttaccggta gccctggcgg gagtcggatt attaccaccg tgctgcaaat
    12421 ggtggtgaat agcatcgatt atggcatgaa tgtcgctgaa gcgaccaatg caccgcgctt
    12481 ccaccatcaa tggttgccgg acgagctgcg tgtcgaaaaa gggtttagcc cggatacgct
    12541 caagctgctg gaagcaaaag gtcagaaagt ggcgctgaaa gaggcgatgg gcagtacaca
    12601 aagcattatg gttgggccgg acggtgagtt gtacggcgca tccgacccgc gctcggtgga
    12661 tgatttaacg gcggggtact aaggttagcg gccctcttcg tgggaagagg gctattttgt
    12721 cagggcaagc cgaaggtagc cttttttatt tcgtaatcct gtagatattc ttccagcggc
    12781 tttgccgttc ccagagaatg aatttcacca ctgtctttat caataataaa aggtgcgtta
    12841 ccagctaagc gcgcggcctc atctccagtt tcgagaaatt ctcgtgcttc gaaacagaaa
    12901 taccagccct ggctaaagcg tccatgtaga gtaatgacga ccgggagatc tgcatcatca
    12961 aggtaatggt tcgctttcgc gaatgcgtcg tgataagtaa t
//
LOCUS       DAZGCS010000125        12895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGCS010000125 DAZGCS010000000
VERSION     DAZGCS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.127"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..399
                     /locus_tag="ACHK01_004124"
     CDS             <1..399
                     /locus_tag="ACHK01_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO8098989.1"
                     /translation="IIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILS
                     PGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNT
                     YCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            723..2111
                     /gene="narU"
                     /locus_tag="ACHK01_004125"
     CDS             723..2111
                     /gene="narU"
                     /locus_tag="ACHK01_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO8098990.1"
                     /translation="MALQNEKNNRYLLRDWKPENPTFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2193..5933
                     /gene="narZ"
                     /locus_tag="ACHK01_004126"
     CDS             2193..5933
                     /gene="narZ"
                     /locus_tag="ACHK01_004126"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO8098991.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5930..7474
                     /gene="narH"
                     /locus_tag="ACHK01_004127"
     CDS             5930..7474
                     /gene="narH"
                     /locus_tag="ACHK01_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8098992.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7474..8169
                     /gene="narW"
                     /locus_tag="ACHK01_004128"
     CDS             7474..8169
                     /gene="narW"
                     /locus_tag="ACHK01_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8098993.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8166..8846
                     /gene="narI"
                     /locus_tag="ACHK01_004129"
     CDS             8166..8846
                     /gene="narI"
                     /locus_tag="ACHK01_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8098994.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8925..9818
                     /locus_tag="ACHK01_004130"
     CDS             8925..9818
                     /locus_tag="ACHK01_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO8098995.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9914..10759)
                     /gene="nhoA"
                     /locus_tag="ACHK01_004131"
     CDS             complement(9914..10759)
                     /gene="nhoA"
                     /locus_tag="ACHK01_004131"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO8098996.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDGWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10932..11501
                     /locus_tag="ACHK01_004132"
     CDS             10932..11501
                     /locus_tag="ACHK01_004132"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO8098997.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDNKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11505..11732)
                     /gene="pptA"
                     /locus_tag="ACHK01_004133"
     CDS             complement(11505..11732)
                     /gene="pptA"
                     /locus_tag="ACHK01_004133"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO8098998.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11831..12895)
                     /locus_tag="ACHK01_004134"
                     /pseudo
     CDS             complement(11831..12895)
                     /locus_tag="ACHK01_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3118 a         3304 c         3264 g         3209 t
ORIGIN      
        1 ataataaaac agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgccct
       61 actatgcagc tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg
      121 gcaattcttt cgcctggtgc atttgaagtt cactctgacg gaatagatgt ggataacgaa
      181 ttgtttcact atcctattaa aaaagcatat accccatata atatacacac ttacaagaca
      241 gaggaagttg taaaccagag gaatataaaa gttaaaaaca tgaccttaga tgaaataaac
      301 aatacttact gtaataacga ttattacaat caggcaataa gagaggaacc gatagacctt
      361 ctggacagat cgttttcctc cagttcatgg cctttttagt gaataaatca tcactaattc
      421 tataagtgtc tggtcttgtt gataatatag ttaatatgta accctttaga ttcacgatct
      481 gaagggttga aacatgacat acgttcaaag attaaccccc gtttgaaggt tagcttgtta
      541 gttacattta gtaacacata tctggctact taatccccat tctgcccccg cggttacgct
      601 ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct aataactggc
      661 tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat gtgaggaaca
      721 atatggcact gcaaaatgag aaaaataatc gttatctttt acgcgactgg aaaccagaaa
      781 atccgacctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc tggatatcag
      841 tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt accgttaatc
      901 tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc gcattaccct
      961 ccgtttctgg cgcattattg cgtgttccct actcctttat ggtgcctata ttcggtggac
     1021 gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg ctcggaattg
     1081 ccgtgcaaaa tccgaatact ccttttggga tatttatcgt tatcgctttg ctatgcggtt
     1141 ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca
     1201 aacaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta agtgtaatgc
     1261 agctggttgc accgctggtc atttttgtac ctgtatttgc ctttctcggc gtcaatggcg
     1321 taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc
     1381 tactggcgat tgccacgatc gccgcctggt cagggatgaa tgatatcgcc agttcacgcg
     1441 cctcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg ctgagcctgc
     1501 tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc atgctggcaa
     1561 aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca tttatcggtg
     1621 ccatcgcgcg gtcggttggt ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt
     1681 tgatcaactt tatttttatg gcgattttca gtgccctgct gttccttacc ttaccgggca
     1741 caggctccgg taatttcatc gccttttacg ccgtatttat ggggctgttt ctgactgcgg
     1801 gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag ataaccattt
     1861 atcgggtaaa gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa
     1921 cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt tttattccgc
     1981 aggcgtttgg catgtcgctc aatatgaccg gctctccggt cggcgcgatg aaagtgtttt
     2041 taatcttcta catcgtttgt gtgctgctga cctggctggt ttatggtcgg cggaagttca
     2101 gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca aggcatcaga
     2161 tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat cgctttcgct
     2221 acttcaaaca aaagggcgaa acctttgccg atggtcacgg acaggtgatg catagcaacc
     2281 gcgactggga ggacagctat cgccagcgct ggcagttcga caaaatcgtg cgttccaccc
     2341 acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga
     2401 cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc aatcatgaac
     2461 ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct aaccgcctga
     2521 aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc aaacaacaca
     2581 gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca
     2641 aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gcaggaacta aaccagctga
     2701 ttgccgccgc taacgtctgg accatcaaaa cctacggccc ggatcgcgtt gccggtttct
     2761 cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacgcgttat ctgtcgctgc
     2821 ttggcggcac ctgtttaagt ttctacgact ggtattgcga cctgccgccc gcctcgccga
     2881 tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagctaca
     2941 tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg
     3001 aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca
     3061 aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagcgccctg gcgatggcaa
     3121 tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact
     3181 actgccgccg ctacagcgac atgccgatgc tggtaatgct ggagcctcgc gacgatggta
     3241 gctacgttcc cgggcgcatg atccgcgcat ctgacctggt ggatggactg ggcgaaagca
     3301 acaatccgca gtggaaaacc gtagcagtta ataccgcagg tgaattggta gtgccgaacg
     3361 gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg
     3421 gtacggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg
     3481 ccttccccta ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg
     3541 tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt cgatggcaac acctgtccgg
     3601 tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg ctggaagatg
     3661 aaaacagtgc gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa
     3721 ttaccggcgt gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc gataccgccc
     3781 ataaaacgca tgggcgctcg atgattatcc tcggcgcagg tgttaaccac tggtatcaca
     3841 tggatatgaa ctaccgtggg atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa
     3901 gcggtggcgg ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa accggctggt
     3961 tgccactggc ctttgcgctc gactggaacc gaccaccgcg ccaaatgaac agcacctcgt
     4021 ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt
     4081 caccgctcgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg
     4141 aacgtatggg ctggctacct tctgcgccgc agctggggcg taacccgctc gggattaaag
     4201 ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg ctgaaatcgg
     4261 gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg cgtaatttgt
     4321 ttgtctggcg ctctaacctg cttggctcct ccggcaaagg ccacgagtat atgcagaagt
     4381 atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca
     4441 aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac ctgctggtga
     4501 cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca
     4561 cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt
     4621 ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg
     4681 ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac
     4741 aacccctact gcatgactct ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc
     4801 gcaaaggcga atgcgatctg attccaggca aaaccgcgcc gaatattgtg gcggtggagc
     4861 gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg gacaaacttg
     4921 gcaatggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc ctcggcaaac
     4981 tcaattacac caagcgtgat ggcccggcac aggggcgtcc gctgattgac accgccattg
     5041 acgcttcaga agtgattctg gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag
     5101 cgtggcaggc gctgggcgag atcaccgggc gcgaacatac ccatctggcg ctgcacaaag
     5161 aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc
     5221 ccacctggtc cggtctggaa agcgatcacg tatcctataa cgcgggatac accaacgttc
     5281 atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt
     5341 ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg
     5401 tcagtgagat gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga
     5461 cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc
     5521 tctctcgcgg tggaccgatt gtctggatca gcgaaacaga tgcccgagaa ctgaccattg
     5581 tcgataacga ctgggtggaa gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg
     5641 tcagccaacg tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa cgcattatga
     5701 atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg gttacccgcg
     5761 tttgcccgaa accgacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact
     5821 actacggcac cgtcggatcg aaccgcgatg agttcatcat gatccgcaag atgaagaacg
     5881 ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg
     5941 ttcacaagtc ggcatggtgc ttaacctcga caaatgtatc ggctgccata cctgttcggt
     6001 gacctgtaaa aacgtctgga ccgggcgcga aggcatggag tacgcatggt ttaacaacgt
     6061 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
     6121 cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt
     6181 aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt
     6241 cactttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc ctactgctcg
     6301 cccgcgctcg ctgattgacg gtaagcggat ggacaaagtg atctgggggc caaactggga
     6361 agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccataca
     6421 aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga
     6481 acactgcctc aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga
     6541 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag
     6601 cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat
     6661 cttctgttat ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg
     6721 tcgcatccgg tatctgggcg tgctgcttta cgacgccgac cgcatcgagg aagcggcgag
     6781 caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa gtgttcctcg atccacacga
     6841 tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgaagctgc
     6901 ccagcgttcg cctgtctaca aaatggcgat ggactggaaa ctggcgctac cgctgcaccc
     6961 tgaatatcgc accctgccga tggtctggta cgttcctccg ctgtcgccga ttcagtccta
     7021 cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg
     7081 tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc cggtactgcg
     7141 ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt
     7201 tactgatact cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg aagagatgta
     7261 tcgttacctc gccattgcca actatgaaga tcgttttgtc atcccgacga gccatcggga
     7321 aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg
     7381 ccacggttcg gacagtaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat
     7441 caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg
     7501 ttgatggagt atccggacga gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc
     7561 cgcagcgacg cgccgatgct tacggatttc acccacaacc tgcttaacgc gccgctgctg
     7621 gataaacagg ccgaatggtg cgaagtgttt gaccgtggac gtaccacatc gctgctgctg
     7681 ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg
     7741 gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta tctaccgctg
     7801 tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt gctcaatgtt
     7861 gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg
     7921 ttgtttgatg ctctgctgca actggcggga agctcccttt caagtgacag tgtcacaaaa
     7981 caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt gtgggaagag
     8041 gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc gcttaatcaa
     8101 tatcagcgac gctttagcca ggatgtcgcg ccgcaatatg tcgacatcag tgcgggaggt
     8161 gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac atttgtgcga
     8221 cggtgttttt cctcggcagc tggctgcgct acgactacgg gcagtacacc tggcgcgcct
     8281 cctcaagtca aatgctcgat aaacgcggga tggttatatg gtcgaatctg ttccatatcg
     8341 gcattttggg gatcttcttc gggcacctgt tcggcatgtt aacgccgcac tggatgtacg
     8401 cgtggttttt gccagtggca gcgaaacaac taatggcgat ggtgctcggc ggtatttgcg
     8461 gtgtgttgac attgattggc ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg
     8521 tgcgtgcaac ttccactacg ccggatatta tcatcatgag cattttgctg atccagtgtc
     8581 tgctggggct aagtaccata ccgttttcag cacagtatcc tgatggtagc gaaatgatga
     8641 agctggtggg ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca gaaatgctta
     8701 acggcgtagc gttcgttttc cgcctgcatc tggtattggg aatgaccatc ttcctgctct
     8761 tcccgttcac cagattggtg catgtgtgga gcgcgccgtt tgagtacttt actcgtcggt
     8821 atcagattgt gcgttcgcgg cgataatttc accctggccg gatagccctt cgctctccgg
     8881 ccacttgcag gctatgatag ttttccagtc acaggagaac gttaatgaaa ccgcaggtgt
     8941 accacgtcga tgcttttacc tctcaaccat ttcgcggcaa ttctgccggt gttgttttcc
     9001 ccgccgataa tcttagcgaa gcgcaaatgc agcttatcgc ccgcgagtta ggccattcgg
     9061 aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac tttacgccaa
     9121 cggttgaagt gccgatttgc ggtcacgcga cagtagctgc gcactatgta cgtgcgaagg
     9181 tgttaggttt aggaaattgc acgatctggc aaacatcgct ggcaggaaaa catcgcgtga
     9241 ctatcgaaaa gcacaacgat gattatcgta tttcgctgga acaaggtacg ccgggctttg
     9301 agccaccgct ggaaggtgaa acacgtgagg cgattatcaa cgcgcttcat ctcactgaag
     9361 acgatatttt gccgggcttg ccaattcagg tcgcaacaac aggacactca aaagtgatga
     9421 tcccgttgaa accggaagtg gatatcgacg ccctttcgcc tgatcttgct gcgctgagcg
     9481 ctatcagcaa acagattggc tgcaatggtt tcttcccgtt ccagatccgc ccaggcaaaa
     9541 acgaaaccga tggtcgtatg ttctcgcctg cgattggtat tgtggaagat ccggtgactg
     9601 gcaatgccaa tggcccgatg ggtgcatggt tggtacatca caacgtattg ccccacgatg
     9661 gcaacgtgtt atgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgac ggcatgattg
     9721 aagtgacggt gacaattcgt gataaccaac cggaaaaagt caccatttca ggcacagcgg
     9781 tgattttgtt tcatgccgaa tgggcaattg aactctgaaa ccgttgcctg atgcgcttcg
     9841 cctgtcaggt ctacaaccga gctaatgtat gccggataag gcgtttacgc cgcatccggc
     9901 aacctgacat aaattatttt cccgcctccg gatgcgtatc aaacgccgcc atcaccagcg
     9961 ctaactcatc cacagtaaag ccatgtttcg catcatccac gcccagacca aactgttcct
    10021 gcatcacggc atataacgac accacatccg gtagatttcg ctgctccacc gcgtgcccat
    10081 tttcataatg ggtaaaatga aaattggtca gcgttagctt gccaccgtcc ggcaaatggc
    10141 ggcacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc
    10201 ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccaat
    10261 gctgatgatg attaaactgc aacacccaac catcaccctc ctgcaacaac cgatactctc
    10321 cgtgtggcgt ggtctgcacg agatcggaaa ctaaacgaat cggcgcggtt agcgtctgcc
    10381 caccgaaacc gacatcagca atccattttt cctcttccag ttccaccaac agcaaacgat
    10441 gggtgcgcgg cggtaatgct ggcggatttg ataacactac gcgccctaac aagctgcgaa
    10501 cgttaaaccc cagctcgcgt aacacccgct caaacacgcc attctgctca aaacagtaac
    10561 cgccacgacg ggctatcacc agtttctctt ccagcgattg atcatcaagc tgtatttccc
    10621 tcggcagcaa aacgtcaagg ttttcaaacg gaatagtgca attgtgtttc aggtgcaatg
    10681 cacgaagcgt atcaatattg accgcagcag ctcccgacca gttaatacgg gcaaaatagt
    10741 gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga
    10801 atagacgccg caccggctat tttgtgcgat cgcggacatg catcacgttt gcaaatcgcc
    10861 aatcccgtta atttcgttac ttattgcgtt acggactacg cttaaaaaaa ccttcttcgc
    10921 gaggcgatac catgagccga ttcatcccca tagaattaca ccatgccagc cgtctgttaa
    10981 atcatggccc aactgtcatg atcaccagtt tcgatgagca atcccagcgg cgtaacatta
    11041 tggcagcagc ctggtcaatg ccggtggagt ttgaaccgcc acgcgtggcg attgtggtag
    11101 ataaatcgac atggaccagg gagttgattg aacgtaacgg taaatttggc atcgttatcc
    11161 cgggcgttgc agcaactaac tggacgtggg cagtgggaag cgtgtcgggt cgtgaagaag
    11221 ataaatttaa ttgctatggc attccggtgg tgaaaggtcc ggtgcttggt ttgccacttg
    11281 ttgaagagaa atgcctggcg tggatggagt gccgcctgct acctgcgact tctgcgcaac
    11341 aaaaatacga cacgctgttt ggcgaggtgg tatcagcagc ggcagacgca cgggtatttg
    11401 tcgaaggccg ctggcagttt gatgataata agctcaatac gttgcatcat ttaggtgctg
    11461 gcacgtttgt tactagcggc aagcgtgtta cggcaggtta agcattacat gctgtaaccg
    11521 ggtttcttta tcaaagcctc catttgaggc gcgatttcgg catcccagat agcttgccaa
    11581 gattctggtt gaatctgctg tagagcaatg cttatcgaac tgtctttact gttcagatgg
    11641 cgaataataa cgtcggtaat atctgcagca agtgctgctt tttgttgttc gtccagttca
    11701 cgcggaaagc atttaatgtc gatgtgcggc atgattacgc tccttttgaa agatgtcagt
    11761 aaaattaaca cacattgtga atgtcatgtg tccggtaaag cgtttatacg acaataatgc
    11821 cagggcaaga ttacgaaagc cttctccccg caaggactga cgccaggtag tttctgtcca
    11881 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
    11941 tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct tttcttattt
    12001 tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc tccacgtgcc
    12061 agtggtttcg gattgctgtg gcgaacttct ctgcggttaa atcagcagaa ctgatataat
    12121 atctgaccgt catttctggc tctttctttt gttctgctat tatggaccga aaggagactg
    12181 ccacgcataa tttcttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga
    12241 catcgcaaac aatatgaaga cggatttctt ctctgccgtg actcttttca ctcattgcgt
    12301 aactgtcatg ctctggatta tttaattctt tcagcggaaa tttttcctca aaggctttat
    12361 ttagccgccc ctggtttcct tttaccgcga ataaataatc acctccctgt ttttgtatct
    12421 tttctgcaat atctttctgg cagcccatcg catcagttgt gatgattttt cctttaatat
    12481 ccatcatgtt aagaagttca ggaatggctg taatctcatt ggatttctca tccgtcttga
    12541 tctgtccgag gaccagacta tgcattgttg agaacgcact aatgacatga atcgctcccc
    12601 tgcgacgact cttgtcataa gagtgccgga gcgtttttcc atcaattgca atgacgtctt
    12661 tatcatctga agaatggcag tcacgcatcc agttaataaa gcactcgtga aattttgcag
    12721 gactgataca ggatacaact ctggcaatga tatcgtgaaa aggaatacca ttttcaaaat
    12781 caccatatca cttcaaaaca tcgggatgtg tttccccaaa atcctctata tcttcccagc
    12841 cttctgcacc agaaataacg gcacaaatag tcaacagtag aatatccgat aattt
//
LOCUS       DAZGCS010000126        12566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGCS010000126 DAZGCS010000000
VERSION     DAZGCS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.128"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            167..5587
                     /locus_tag="ACHK01_004135"
     CDS             167..5587
                     /locus_tag="ACHK01_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO8098999.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASQTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEE
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5796..6062
                     /gene="hipB"
                     /locus_tag="ACHK01_004136"
     CDS             5796..6062
                     /gene="hipB"
                     /locus_tag="ACHK01_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO8099000.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6062..7384
                     /gene="hipA"
                     /locus_tag="ACHK01_004137"
     CDS             6062..7384
                     /gene="hipA"
                     /locus_tag="ACHK01_004137"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO8099001.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7696..7904
                     /locus_tag="ACHK01_004138"
                     /pseudo
     CDS             7696..7904
                     /locus_tag="ACHK01_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8336..8440
                     /locus_tag="ACHK01_004139"
                     /pseudo
     CDS             8336..8440
                     /locus_tag="ACHK01_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8603..9313
                     /locus_tag="ACHK01_004140"
     CDS             8603..9313
                     /locus_tag="ACHK01_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8099002.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAVVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9355..12006
                     /locus_tag="ACHK01_004141"
     CDS             9355..12006
                     /locus_tag="ACHK01_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8099003.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIALGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            12020..12550
                     /locus_tag="ACHK01_004142"
     CDS             12020..12550
                     /locus_tag="ACHK01_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099004.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
BASE COUNT         3501 a         2700 c         3181 g         3184 t
ORIGIN      
        1 ttttttaatc tatcaattcg aaagtattag caaattagcc aaattgacac cttcccacct
       61 ccaactaaca cttaatacag acctctgaca atttaataag tttcattttt aatgtctgac
      121 gactcatctc tttcgtggct gtattgtttg tatatggagc gtttatatga atagaatcta
      181 tcgcgtgata tggaattgca ctctacaggt atttcaggcc tgctcggaat taactcgcag
      241 ggcaggtaaa acatcgacgg ttaatttgcg taaatcctct ggactgacaa cgaaattcag
      301 tagattgacg ctgggtgttt tgctggcact aagcggttca gcgtctggtg caagtctgga
      361 agttgataat gatcagatta ccaatattga tactgatgtt gcttatgatg cctacctggt
      421 tggctggtat ggcactggag tgcttaatat tttggctggc ggtaatgcct ccttaaccac
      481 tattactacc agcgtcattg gcgctaatga ggactcagag ggcaccgtta atgttttggg
      541 tggcacctgg cgattgtatg atagcggaaa taatgcaagg cctttaaatg tgggtcaatc
      601 cggaacgggg acgctgaata ttaaacagaa gggtcacgtc gatggaggct atttaagatt
      661 aggttcttcg acaggaggcg tcgggacggt caatgttgag ggagaggact ctgttctgac
      721 gaccgaatta ttcgaaatag ggagttacgg tacgggctca ttaaatatta cggataaggg
      781 ttacgtcacg agttcaatcg tcgccatttt aggctatcag gcgggcagta atggccaggt
      841 tgtcgttgaa aagggtggcg agtggctaat aaaaaataac gattcctcaa ttgaatttca
      901 aattggtaat caaggaactg gggaggcgac tattcgcgag ggtggactga ttacggctga
      961 aaatacgatt atcggtggca atgccaccgg tgtcggaacc ctgaatgtgc aggatcaaga
     1021 ctctgtcatc acggtacgca gactctataa tggatatttc ggtaatggca cactcaatat
     1081 ttccaataat ggactaatta ataacaaaga atattcatta gtgggcgttc aggacggttc
     1141 ccacggtgtc gtcaacgtga ccgataaagg gcattggaat ttcctcggaa cgggcgaagc
     1201 tttccgctat atctatatcg gtgatgctgg cgacggtgaa cttaatgtct cgcgtgaagg
     1261 taaagtagat tcgggaatta tcactgcggg gatgaaagaa acaggcacag gcaacattac
     1321 tgttaaggat aagaactccg ttatcactaa tctcggaact aatcttggtt atgacggcca
     1381 cggcgaaatg aatatcagta atgaagggct tgttgtcagc aacggaggaa gctcactcgg
     1441 ttatggagaa accggcgtcg ggaatgtcag catcaccacg ggggggatgt gggaggtcaa
     1501 taagaatgtc tataccacca ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg
     1561 cggtaagttc gtatcgcaaa atattacttt tttgggcgat aaagcaagcg gtatcggcac
     1621 actgaacctg atggatgcga catcatcgtt cgatactgtg ggtatcaatg tcggtaattt
     1681 tggtagcggt atcgtaaatg tcagtaatgg tgccaccctt aattcaacgg gctatggatt
     1741 tatcggagga aatgcctccg gtaaggggat agttaatatt tcaacggaca gtctctggaa
     1801 tttaaagaca tcatctacta acgcacaatt gctacaggtc ggtgtattag gcaagggtga
     1861 actgaatatt accaccggag gtatagttaa agcgcgtgat acacagatag ctctcaatga
     1921 caaaagtaag ggcgacgtga gggtggatgg gcagaactct cttcttgaaa cattcaatat
     1981 gtacgtaggg acatctggta cgggtacgtt aaccctgacg aatagcggca cgctgaatgt
     2041 cgaaggtgga gaagtttact taggtgtttt tgaacctgct gtaggaacgc taaacattgg
     2101 tgcagctcac ggtgaggcgg cggcagatgc cgggtatata accaacgcga cgaaagtaga
     2161 gtttggttct ggcgaaggtg tttttgtctt taatcatact aataacagtg atgccggcta
     2221 ccaggtcgat atgctgatta caggtgacga taaagacgga aaagtgatcc atgatgcagg
     2281 ccatacggtg ttcaatgcag ggaatactta tagcggtaaa acgctggtca atgacggcct
     2341 cctgaccatt gcgtctcaaa cggcagatgg ggtaacgggc atgggttcga gtgaagtcac
     2401 cattgcaagc cccggtacgc tcgacattct cgcatcaacg aacagtgcag gagattacac
     2461 gctgaccaat gcgctcaaag gcgatggctt gatgcgagtg cagctgtcat cctacgacaa
     2521 gatgtttggc tttacccatg caacagggac tgaattcgcc ggtgttgccc aactgaaaga
     2581 cagtaccttc actctggaac gcgacaacac cgctgcgctt actcacgcga tgttgcagtc
     2641 tgacagtgaa aataccacat cggtaaaagt gggagagcaa tccattggtg gactggccat
     2701 gaatggcggt acgctcattt tcgatacgga tattcctgct gcgacgcttg cagagggata
     2761 tatcagcgtc gatacgctgg ttgtcggcgc gggtgactac acctggaaag gccgtaacta
     2821 tcaggtaaac gggacgggcg acgtgcttat cgacgtgcct aaaccgtgga atgatcccat
     2881 ggcgaataac cctctgacga cgctcaattt gctggaacac gacgatagcc atgtcggcgt
     2941 tcaactggtg aaggcgcaaa cggttattgg gtcgggtggc tcattaacgt tacgtgattt
     3001 acagggcgac gaggtggaag cggacaaaac gttacacatt gcgcaaaacg gaacggtggt
     3061 cgccgagggt gattatggat tccgcctcac gaccgcacca ggtgatggtt tgtacgttaa
     3121 ctatgggctg aaagcgctga acatccatgg tgggcaaaag ctgacgttag ccgaacatgg
     3181 cggagcctat ggcgcaacgg ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc
     3241 aatcaatacg gtgcgacagg tttcgctttc caacggtcag aacgactatc agggggcaac
     3301 ctacgttcag atggggacat tacgtaccga tgcggatggt gcacttggca acacccggga
     3361 actgaacatc agcaacgcgg ccatcgtcga tcttaatgga tcgacgcaga cggtagagac
     3421 attcaccggg cagatgggtt cgactgtttt gttcaaagag ggggcgctga cggtaaataa
     3481 aggtgggatc agtcagggtg aactgacagg tggcggaaac ctgaatgtta cagggggaac
     3541 gctggctatc gaggggctta atgcacgcta caatgcgtta accagcatta gcccaaatgc
     3601 ggaagtcagc ctcgataata ctcaggggtt aggcagagga aatattgcca atgacggtct
     3661 gttaacgcta aaaaacgtga ctggcgaact gcgtaatagc ataagcggga agggtatcgt
     3721 gagcgcaacc gccaggacag atgtagagct ggatggcgat aatagccgct ttgtggggca
     3781 attcaacatt gatacaggca gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc
     3841 ttccgttatc aataatggcc tgctcaccat ctccactgag cgtagctggg cgatgacgca
     3901 cagtatcagc ggtagcggtg atgtgacaaa actgggtacc gggatcctga ctcttaacaa
     3961 cgattccgcg gcgtatcagg gtacgacgga tatcgtgggg ggggaaattg ctttcggttc
     4021 cgactctgcc attaatatgg caagtcaaca cattaatatc cataacagcg gtgtgatgtc
     4081 gggaaatgtc accactgcag gtgatgtgaa cgttatgcct ggggggacac tgcgtgtcgc
     4141 taaaaccaca atcggcggca acctggagaa tggcggcacg gttcaaatga atagcgaagg
     4201 ggggaaaccg gggaatgtac tgaccgttaa cggcaactat accggaaaca atggcctgat
     4261 gacgttcaac gcgacgctgg gcggcgataa ttcgcccacc gataaaatga acgtgaaagg
     4321 cgatacccaa gggaacactc gcgttcgggt tgataacatt ggcggcgtcg gtgcacaaac
     4381 ggtcaacggt attgaactca ttgaggttgg cggtaattct gcaggtaact tcgcgctgac
     4441 caccggaact gtcgaagctg gggcttacgt ctacacgctg gctaaaggga aggggaatga
     4501 cgagaaaaac tggtatctga ccagtaaatg ggacggcgta acgccaccgg atacgcccga
     4561 ccccatcaat aatccccctg ttgtggatcc ggaaggccca tcagtttatc gcccggaggc
     4621 cggaagctat atcagcaaca ttgccgcagc caactcgctg tttagccatc gtttacacga
     4681 ccgtctgggt gagccgcagt atatagattc actgcattct caggggtcgg caagcagtat
     4741 gtggatgcgt catgtcggag ggcacgaacg ttcaagggcc ggtgacggtc agctaaatac
     4801 tcaggctaac cgctatgtat tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc
     4861 gcaggatcgc tggcatcttg gcgtgatggc aggttacgct aatcagcaca gtaatactca
     4921 gagtaatcgt gtgggttata aatcggatgg gcgcatcagc ggttacagcg ctgggctgta
     4981 cgcgacctgg tatcagaacg atgcgaataa gaccggcgct tatgttgaca gctgggcgct
     5041 gtataactgg tttgataaca gcgtcagttc cgataaccgt tctgctgacg actatgattc
     5101 tcgcggtgtg acggcctctg ttgagggtgg gtataccttt gaagcgggaa catttagcgg
     5161 cagcgaagag acgctgaata cctggtacgt ccagccacag gcgcaaatca cctggatggg
     5221 tgtgaaagat tccgaccata cccggaaaga cggaacgcgc attgaaacgg aaggcgacgg
     5281 aaatgtgcaa acgcgacttg gggtgaaaac ctacctgaac agccatcacc agcgtgacga
     5341 tggtaaacag cgtgagttcc agccttacat tgaagcgaat tggatcaaca acagcaaagt
     5401 ctacgccgtg aagatgaatg gtcaaaccgt aggccgtgaa ggtgcgcgta atctcggtga
     5461 agtacgtacc ggggttgagg cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg
     5521 tgtgcaacta ggtgataaag gctatagcga tactcagggc atgctgggag tgaaatatag
     5581 ctggtaaacc gtataagccg catgtcgaga tggcatgcgg cttaatattg ccgacttcaa
     5641 aggctttaaa gcctcccttt tatccgcgat cgcggatatc gcagcgttta tcccgtacag
     5701 cggataagat gtgtttccag attgacttta tcctcactaa aggataaaac tgataatatc
     5761 cccttaagcg gataaatttg ctgtggacgt atgacatgat gagctttcag aagatctata
     5821 gcccaacgca attggcgaat gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga
     5881 gcgagctggc gaaaaaaatt ggcattaagc aggcgacaat ttccaatttc gaaaacaacc
     5941 ctgacaatac cacgctcacg acatttttta agattttaca gtcgcttgaa ctctcaatga
     6001 cactatgcga cacgaaaaat gcctcgccag aatcaacaga acagcaagat ctggagtggt
     6061 aatgcctaaa cttgtcactt ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc
     6121 caacggcgcg cacaccttta agtatgcacc ggagtggtta gccagccgtt atgccagacc
     6181 gttgtcactt tcgctgccat tgcagagggg gaatatcacc tcagatgccg tatttaactt
     6241 cttcgataac ctgttacccg atagcccgat tgtacgtgac cggatcgtta aacgttatca
     6301 tgctaaatcc agacaaccgt ttgatttatt gtcagaaata gggcgagaca gcgttggtgc
     6361 cgtaacgtta ttacccgaaa atgaaaccat aacgcgcccg ataatggcat gggaaaagct
     6421 tactgaagcc aggcttgaag aagtattaac ggcttataaa gcagatatcc ctctgggcat
     6481 gattagagaa gaaaatgact ttcgcatctc ggttgctggc gcgcaggaga agacagcgct
     6541 gcttagaata ggcaatgact ggtgcattcc gaaaggaata acgccgacga cgcacatcat
     6601 taaattaccg attggcgaaa tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt
     6661 tgataatgag tattactgtc tgctgctggc gaaagaactt gggttgaatg ttccggacgc
     6721 agaaatcatt aaagcgggaa gggtgcgcgc gttagcggtc gaacgttttg acaggcgttg
     6781 gaatactgag cgaacggttt tacttcgctt gccacaggag gatatgtgtc agacattcgg
     6841 tttaccttca tctgtgaaat atgaatcaga tggaggccca ggcatcgcgc ggatcatggc
     6901 gtttttgatg gggtccagcg aggcgcttcg cgatcgttat gattttatga aattccaggt
     6961 cttccagtgg ttgattggcg caacggatgg ccatgcaaaa aacttctccg tatttattca
     7021 ggctggcggc agttatcgac tcacgccatt ttacgacatc atttcagcat ttccggtcct
     7081 tggcggtacg ggaatacaca tcagcgatct caaactggca atggggctta acgcatccaa
     7141 aggcaaaaaa acggcaatcg ataaaattta tccgcgacat tttttggcga cagcaaaggt
     7201 gctgagattc ccggaagtgc agatgcatga aatcctaagt gactttgcca gaatgattcc
     7261 ggcagcactg gataacgtga agacttcatt accgacagat tttccggaga acgtggtgac
     7321 ggcagttgaa accaatgtgt tgaggttgca tggacggtta agccgagaat acggtagtaa
     7381 gtgagatatg gggtttggtc gttgttaatg agcacgacaa tcatgaccgc caacgactgg
     7441 attcttcctt ggtttatact ggtgcgcata atgtatatta tgttaaatca tgtctgagtg
     7501 atgcgaagtg cttttctggt agtcgttatt cgttcagggt tcaagaactt aagcctctcc
     7561 tctttcataa ttggcctgtg catggagaaa tagcctggtt attccctgcc ccatgctaag
     7621 agcgcagtgt ctgtcgcata gacactgcat acgaaaacac cagagggtgc tgttacttat
     7681 ttaaggacgg atcaagcggg aatgagtcca ctccggtatc agaaatggct acgtctcaat
     7741 gaagtcaggc gaccgatgct gaatgaacat tacgatgtca ccacagcagc ctatgctgtc
     7801 ggttacgaaa gctatcccat ttcagtcggg aatattcgcg gatgtttgga gagtcaccca
     7861 agagagatat taccaggtta agaaagtctg tcggtcagct ttaataaacc ctgcacttat
     7921 ctgtttagag caattcggtg ttagtttcag caagcaaaca ttaaccatag ctaatgattt
     7981 atagccatat taaccattgg tagcccttga cctttcctga cacttccctt gttcgcaata
     8041 atattctgtt tagtcacggt ttataaataa catgcataat gaaatcgtca tgttatttag
     8101 cgacataaaa tgtttttgca aatactctct tcaacaagtg cacaataaac ttgttgtgat
     8161 taggtgtcct tgtgatttaa agatattatc gttcttcgtg aaatgtaata accctggaaa
     8221 ctatattctc agggcaacaa ccaatcaaga atgattcttg tttttaagca ataacagaat
     8281 gacttaattg ttaaccctct ttattcagct acttaaatat aaattttgga gaattatgaa
     8341 actcaaacat gttggtatga ttgtcgtttc tgtgttggcg atgtcgtctg ctgcggtaag
     8401 cgcagccgag ggtgatgaat cagtaacgac cactgttaat ttactttgta gcaaggtttt
     8461 cctattaaaa gagaaccaac atttattaca aacgcattaa gtctggaaac ccggcgactc
     8521 gccaatgtat tagcaacatt aatcgattgt ctgatgtaac ggttttcccg cttatcctct
     8581 taacacatgg agaatgcatc agatgcaaac tacgcgtacc ccctactcta tttcctttat
     8641 ggcgacagtg ttacttttat tgctctttgc ttgccatagc accgttgcca acgcagttgt
     8701 cgcactgggt gcgacgcggg taatttatcc ggcaaaccag aaacaggttt tattgcctgt
     8761 cacgaataat gatcctgcca gtgtctatct cattcagtca tggatagaaa atgcaggtga
     8821 ccaaaaagac acccagtttg tcattacccc accgcttttt tccatgcaag gtaaaaaaga
     8881 gaatacgctg cggataatca acgctactaa tcatcagtta ccaggcgacc gtgaaagcct
     8941 gttctgggtg aatgttaaag ctattcctgc tatggaaaaa gaccagaaga atgagaatac
     9001 attacagctg gcaattatta gccgcataaa aatgttttat cgccccacga acctggcaat
     9061 ggcacctgaa gaagcgccag caatgcttcg gtttcgtcgc agcggtagca aactcactct
     9121 tattaatcca acgccctact tcattaccgt aacaaatatg aaagcaggaa atagcaatct
     9181 cccaaacacc atggtgccgc caaaaggtga agtatcggtg gatatcccgc atgctgcaac
     9241 cggggatatc agttttcaga cgatcaatga ttacggtgca ttaactccgc gtattaaggc
     9301 aaccatgcag taagccagtg acttaattaa taataaactc aatcggaatt aataatgaca
     9361 gcctttcgtg ccgcatttaa ggcatatcgt atgcatcagg tgttaatact accacgcttt
     9421 gcacggctta ccattgccct tggcttagca actgcggttt ttcccgttga tgcagagtat
     9481 tattttaatc cacggttttt aagcaatgat cttgctgaat ctgtcgattt atccgccttt
     9541 actaagggac gcgaggcacc acctggtaca tatcgggtcg acatttattt gaacgacgaa
     9601 tttatgacta gtcgggatat tacttttatt gcagatgata ataacgcaga tctcatccca
     9661 tgtctgagta cagaccttct tgtcagtctt ggcataaaaa aatctgcgtt attagacaat
     9721 aaggaacatt ctgcagaaaa acatgtgccg gacaacagcg catgcacacc acttcaggat
     9781 cgtttggccg acgcatcaac tgagtttgat gttggtcagc aacatttgtc actctccgtt
     9841 ccgcaaattt atgttggcag aatggctcgc ggctatgttt ctcctgaact gtgggaagaa
     9901 ggaataaatg ctgggcttct aaattatagt tttaatggta attccattaa taatcgtagt
     9961 aaccataatg caggaaaatc caactatgca tatttgaatt tacagagtgg catcaacatt
    10021 ggtagttggc gactgcgcga taactcaacg tggagttata acagtgggag tagcaattca
    10081 tctaacagca ataaatggca gcatatcaat acgtcggctg aacgtgacat tattccctta
    10141 cgctcacggt taacggtagg tgatagttat accgatggcg atatttttga tagtgtgaac
    10201 tttcgtggcc tcaaaataaa ttcaacagaa gcgatgttgc ccgatagcca acatggtttc
    10261 gctccggtga ttcatggtat tgcccttggc accgcacaag tgagtgtaaa acagaacgga
    10321 tacgatgttt atcagactac tgttccaccc ggccctttta ctattgatga tatcaactct
    10381 gccgccaatg gtggtgatct gcaagtaacc ataaaagagg cagacggcag tattcagaca
    10441 ttatatgttc cttattcgtc tgttccggtt ctccaacgtg ctggatatac gcgttatgcg
    10501 cttgccatgg gggaatatcg tagtggaaat aacctgcaaa gctcccccaa gttcatacaa
    10561 ggtagcttga tgcatggact ggaaggaaac tggacacctt atggcggaat gcaaattgca
    10621 gaagattatc aggccttcaa ccttggtatt ggtaaagatt taggactttt tggtgccttt
    10681 tctttcgata tcacgcaggc caatacgaca cttgcagatg gcacccgtca cagcgggcaa
    10741 tcggttaaat ccgtctacag caaatccttc taccagacag gaaccaatat ccaggtcgca
    10801 ggatatcgct attctacgca aggtttttat aacttatccg acagtgccta cagtcgaatg
    10861 agtggttaca ccgtcaagcc tcctaccgga gacaccaatg agcagacaca atttattgat
    10921 tattttaatc tgttctacag taagcgtggt caggaacaaa taagcatctc tcagcagctt
    10981 ggaaattacg gtacgacatt tttcagtgcc agtcgccaaa gttactggaa cacgtcacgc
    11041 agcgaccagc aaatatcatt tggattaaat gtgccgtttg gtgatattac gacttcgctg
    11101 aattacagct attccaataa tatatggcaa aacgatcggg atcatttact cgcttttacg
    11161 cttaatgttc ccttcagtca ttggatgcgt acagacagtc agtcggcatt tcgtaattca
    11221 aacgccagtt acagtatgtc aaacgatttg aaaggcggca tgaccaatct atcgggggtt
    11281 tatggcactc tgctgccgga taataacctg aattatagcg ttcaggtcgg taacacccac
    11341 ggaggtaata catcgtctgg caccagtggt tacagttctc ttaattatcg tggagcttac
    11401 ggtaatacga atgtcggtta cagtcggaat ggtgacagca gccagattta ttacggaatg
    11461 agtggtggga ttattgctca tgctgatggc atcacctttg gacagccgct gggcgacaca
    11521 atggttctgg ttaaggctcc tggcgctgat aatgtcaaaa tagagaacca gaccggaatt
    11581 cataccgact ggcgtggcta tgccatatta ccatttgcga cagaatatag agaaaaccgt
    11641 gttgctctta acgcgaattc ccttgcagat aatgttgaac tggatgaaac cgtggtcact
    11701 gtcatcccaa ctcacggtgc tattgccaga gcaacattta atgcacaaat cggcgggaaa
    11761 gtattaatga cgttgaagta cggtaataag agcgttccat tcggtgcaat tgtcacacac
    11821 ggagagaata aaaatggcag cattgtcgca gaaaacggtc aggtttatct gactggactt
    11881 ccacagtcag ggaaattaca ggtttcatgg ggcaaagata aaaactcaaa ctgtattgtc
    11941 gagtacaagc ttcctgaagt ttctcctggt accttactga accagcagac agcaatctgt
    12001 cgctaaggaa caatagatta tgaaatacaa taacattatt ttcctcggtt tatgtctggg
    12061 gttaaccacc tattctgctt tatccgcaga tagcgttatt aaaattagcg ggcgcgtcct
    12121 cgattatggc tgcacagtct catcggattc gcttaatttt accgtagatc tccaaaaaaa
    12181 cagtgccaga caatttccaa cgaccggtag cacaagtcca gtcgtccctt ttcagattac
    12241 gttaagtgaa tgcagcaaag ggacaacggg ggttcgggtt gcatttaacg gtattgagga
    12301 tgcagaaaat aatactttgt tgaaactgga tgaaggaagc aatacggcct ccggtttggg
    12361 tatagaaata ttggacgcaa atatgcgtcc ggtgaaactg aatgaccttc atgccgggat
    12421 gcagtggatc ccactggtac cagaacagaa caatattttg ccttactccg ctcgtctgaa
    12481 gtcaactcag aagtccgtca atccgggact ggtgagggct tcggcaacct ttacccttga
    12541 atttcaataa tcagagtcag atatga
//
LOCUS       DAZGCS010000127        12220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGCS010000127 DAZGCS010000000
VERSION     DAZGCS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.129"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..452)
                     /locus_tag="ACHK01_004143"
                     /pseudo
     CDS             complement(1..452)
                     /locus_tag="ACHK01_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(648..1008)
                     /locus_tag="ACHK01_004144"
                     /pseudo
     CDS             complement(648..1008)
                     /locus_tag="ACHK01_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1070..1760
                     /locus_tag="ACHK01_004145"
                     /pseudo
     CDS             1070..1760
                     /locus_tag="ACHK01_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1948..2448)
                     /locus_tag="ACHK01_004146"
                     /pseudo
     CDS             complement(1948..2448)
                     /locus_tag="ACHK01_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            3491..3655
                     /locus_tag="ACHK01_004147"
     CDS             3491..3655
                     /locus_tag="ACHK01_004147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099005.1"
                     /translation="MPLITHLLNVEELSRLLKNVALSVINLFILDDVSEKMEYIPDWV
                     VVTRLNIWWR"
     gene            3628..3816
                     /locus_tag="ACHK01_004148"
                     /pseudo
     CDS             3628..3816
                     /locus_tag="ACHK01_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            3795..3968
                     /locus_tag="ACHK01_004149"
                     /pseudo
     CDS             3795..3968
                     /locus_tag="ACHK01_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            4120..4665
                     /gene="cobU"
                     /locus_tag="ACHK01_004150"
     CDS             4120..4665
                     /gene="cobU"
                     /locus_tag="ACHK01_004150"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO8099006.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4662..5405
                     /gene="cobS"
                     /locus_tag="ACHK01_004151"
     CDS             4662..5405
                     /gene="cobS"
                     /locus_tag="ACHK01_004151"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO8099007.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5417..6496
                     /gene="cobT"
                     /locus_tag="ACHK01_004152"
     CDS             5417..6496
                     /gene="cobT"
                     /locus_tag="ACHK01_004152"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO8099008.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6558..7493
                     /gene="ldtA"
                     /locus_tag="ACHK01_004153"
     CDS             6558..7493
                     /gene="ldtA"
                     /locus_tag="ACHK01_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8099009.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(7548..7623)
                     /locus_tag="ACHK01_004154"
     tRNA            complement(7548..7623)
                     /locus_tag="ACHK01_004154"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7588..7590),aa:Asn,seq:gtt)
     gene            7950..8867
                     /gene="nac"
                     /locus_tag="ACHK01_004155"
     CDS             7950..8867
                     /gene="nac"
                     /locus_tag="ACHK01_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO8099010.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            8969..9919
                     /gene="cbl"
                     /locus_tag="ACHK01_004156"
     CDS             8969..9919
                     /gene="cbl"
                     /locus_tag="ACHK01_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO8099011.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(9957..10032)
                     /locus_tag="ACHK01_004157"
     tRNA            complement(9957..10032)
                     /locus_tag="ACHK01_004157"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9997..9999),aa:Asn,seq:gtt)
     gene            10193..11680
                     /gene="yeeO"
                     /locus_tag="ACHK01_004158"
     CDS             10193..11680
                     /gene="yeeO"
                     /locus_tag="ACHK01_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO8099012.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            11781..11856
                     /locus_tag="ACHK01_004159"
     tRNA            11781..11856
                     /locus_tag="ACHK01_004159"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11814..11816,aa:Asn,seq:gtt)
BASE COUNT         3135 a         2593 c         3096 g         3396 t
ORIGIN      
        1 acttttgtta tgaaataaac atcagtatcg gcaaagcttg ctgccagcgc ctggttacaa
       61 ccttttcccc ccggtgaaaa tataaaatta cttgccagaa gcgattctcc gatagtggga
      121 agcctcggca aataactgat gatatcaaca ttaaacgaac caataacgca tacttgactt
      181 ttcatagttg agtggccttt ttttattccc taggctgtat cataattttc aagcgcataa
      241 gcgatttcat ttttcgcaat agtatcaagc gagtctagga cgataaaagc accgccatga
      301 tatcgagtca gatagccaag tctggcaagt tcattaagat ctgatcgaat agtttctttc
      361 gttacattga ggctattgga cagatcggat acttttacag aaccgaattt actgagtcgt
      421 ttcaatattt catgatggcg ttcaaatttc atgcttcacc tcacttcagt tacgttaata
      481 tctgtcgatg tatattgatg tgataatcac cataaagtgg cggggaaaag caaaaaaaat
      541 caaaaatagc cggagaaaag gtaagcgtta atggagaatc atattgatgc ttatttattg
      601 gtgagaacta cgttccatgg caggagttca tcaatacggt tggaaggtta ttccggcagt
      661 acgctcagga tatggcgcag atactcttcc ggatcaatgt cgttcagaag gcaagtgccg
      721 ataagcccat acaacagtgc actgcgctcg ccgtcgaaga acataaagtt tttatttccg
      781 agacagatgg cacaaagggc tttttccgcg gcattatgtc tgcctccgcc tgaccgtcaa
      841 cactgtaata acagagtgcg ccccactgat tcagtacaca gctgaaccat tctcccagtc
      901 tggatttttg gacagcgtgg catttttctc caccatccac gcatgcagtg agtccagtaa
      961 cagtttgctc ttcctctgcc tgaccacaag atgttctggt tctggcatac tgaatttggc
     1021 cacctgagca taggtaatat gctcacctta gaacaacaca gatgctccaa tgaaaaaagg
     1081 aaatttcagc gcagagttta aacgcgaatc cactcaacta gttgttaacc agaactttac
     1141 ggtggcaggt accatccaaa gctatggatg tcggtctttc cacaatgaca agataagtca
     1201 aacaactgcg taatgagcat cagagcaaaa caccaaaagc ctctctgatg acaccagaac
     1261 aaatcgaaat acgtgagctg aggaaaaagc ggcaacgtat tgaaatagag aatgaaatat
     1321 tagaaaaggc tacggcgctc ttgatgtcag actccctaaa cagttctcga taatcgagaa
     1381 gctcagagcg cattatctgg tgatagcact ctgccatgtg tgcaggattc atcgaagcag
     1441 ctacagagat tggaaaaacc gtcctgaaaa aaagacggta gacgggctgt attacgtagt
     1501 caggtaattg agcttaatgg cggagcaagg aacatcgcca caattgcaac ccggagagac
     1561 taccagacgg gacgcaagct tgctggcagt ctcatgaaag aacaggggct ggtcagctgt
     1621 cagccgttga ccctccggta caggcatggc agtcatgaac ttgtagctat cctaagaact
     1681 tgaagggcgc ttagctgtga cacagctaaa tcaagtatgg tgacgtgacc tatatctgga
     1741 ctggtaaacg atggggatac ccgcttacgc gagagcaata agtggctggc caccattctc
     1801 gatacaaaac tctgctgaat agcagcgatt ttcccagcgt ggtgcgtctt ttgcatttta
     1861 ctggttctga gttatcaatt atctgttcac taatgactac tctaaaaagt tgtatcaatt
     1921 taagtgatat agggagtccg gagattcacc ctttttggtg atgaagttaa caataccgcc
     1981 aattgcctgt gaaccgtaca gtacggaata tggacctttc actacctcaa cacgctccag
     2041 cgcagactca tctatcaaca gtcccacacc ataattatct ccggcgcgct gataagttac
     2101 ctcctgacca tcaatgagaa ttaaaacacg ggaggatgct tcgccacgaa tgcggatttg
     2161 tttacggcct gccaaggagt tgtctgttat ctctactccg gggatgtcct gcaaattatc
     2221 ggctatggag gcattggtgg agttctgcaa tgtttgttgg tcaataacct gtatagtcgc
     2281 cgggctttcc caaaggttgc ttgccgaacg attcgtggtc acgacgagcg tctcttcgtt
     2341 gctgattttt gtttctgaag aagttgcggt tgcagtagaa actatcccag aaatgcataa
     2401 tgacagagtg gaaaggatat aaattcgaca aggtatatta tataatattt catccctcat
     2461 taatacaaag tacaaatgat tgctgtaatg ataattattt tcatttatgt tctcatatat
     2521 ctataattat tgtcatcttc ataaattgta aatatcaaac atttttgacc ttaatcatgt
     2581 tttttgatag atattttacg actgaacata tccgtcgttt cctctgactc gtgtcagtcg
     2641 gtctctggtt gtatccacgg accgcactct tgacataaga gtgcggtccg tggaaaacgg
     2701 agttgaaaaa atggtgtctg aaagaaggta ccatcattgt gatgacaaat actttggcgt
     2761 ggataatgta attaattgct gaaattgacc gtatatgcgg taagactcat atcagtatca
     2821 ggtccggtta attgctgata ctaatataaa ctttttacaa aaggatgaat gcagaaggtt
     2881 actctaatgg ccagagtgat gacaatgatg gtcagacgtt gacctgcaga aactgcattt
     2941 taattggtct ataaagtctg caagaggaat cagggtcaga tcagtgagtc ttatcgcggc
     3001 ccgaagctat gactgaaaga aggctggata cagaattgca acaatttggc aatgtcagaa
     3061 cacagaagct ttgcaagcag gtgaaaggtt atgtctagca aaaggagtat atcagaatat
     3121 atctactgat gtacaaaaca caacaatagc ttcatgatat ataaatacgt gatgacgagt
     3181 ggtactccgc atggttattt aacttctcag caccactaat gatcctgagc aacttcgtat
     3241 ttgggccatt accatggagt agaaagctgt gtctgagaat gcagaatttg agaaaagaat
     3301 tcgctttctt ggagagcttc tgaaaatggc acgctaacaa cgttttgtta aaaaatgtga
     3361 atcacttgcc ggggctgcat tttctattcg aggagaatgt tgacgtagat attacagaaa
     3421 tctcagcgca tcaggataaa ctgctacctt aggtcaacca ccaaaagaaa atacaatacg
     3481 tccagtttgt atgcctttaa taacacatct tctaaatgtt gaagaattat cccgcctgct
     3541 gaagaacgtt gctctgagtg tgataaacct ttttattctt gatgatgtca gcgagaagat
     3601 ggaatatatc cccgactggg tcgtggttac ccggttaaac atctggtggc gctaataaat
     3661 ctggcaagtc acattctgtt gaatgctgtg acagtgcctt accgagagag cgaaattgtg
     3721 gtgttctagt gcatagtcgc caccattccg gataattcta ttacgttgtt ttacaagttc
     3781 ttttacagcg cagatctgtc gctgacgttg aatcaatagc ctggatttca gaacacaatg
     3841 cccgtttgct ggttgttccg gcagtagcat taattcatta tctgaatgaa atgtgtcaat
     3901 ccacagaaac cgctgtcaat gacgcatttt gtcggacgtt aacacgagaa agtctggcta
     3961 ttccgtaatg ccatcttatt tcgtaatgga ttgagaatgc tggattttta attatattac
     4021 gcgcagtatt aatcgcgtaa atgttttgcg gtcagcaacg gtgggggatc cattgttctg
     4081 ttcaatgaat accatcatac atttgaatca ggagtcggta tgatgatttt ggtgacgggg
     4141 ggcgcacgga gcgggaagag tcgccacgca gaggcgctta ttggggactc ttcacaggtt
     4201 ctgtatatcg ctacctcgca aatccttgat gatgagatgg ctgcacggat agaacatcat
     4261 cggcaaggcc gcccggagca ctggcgcaca gtggagcgct ggcaacatct tgatgaatta
     4321 attcatgcag acattaaccc gaatgaggtt gtgttgcttg aatgcgttac cacaatggtg
     4381 actaatctgt tgtttgatta tggcggcgat aaagaccctg atgaatggga ttatcaggcg
     4441 atggaacagg tgattaatgc tgagattcag tcgttgattg ctgcctgcca acgttgcccc
     4501 gcaaaggttg tattagtgac taacgaagtg ggaatgggga ttgtgccgga gagtcgtctg
     4561 gcacgacatt ttcgtgatat tgccgggcgg gtaaatcagc agttggccgc tgcggcaaat
     4621 gaagtatggc tggtggtttc gggtattgga gtaaaaatca aatgagtaaa ttattttggg
     4681 cgatgctctc atttattacg cgtctgcctg taccgcgtcg ctggtcccag ggactggatt
     4741 tcgagcatta ttctcgcggt attattactt ttcctttgat tggattattg cttggcgcga
     4801 ttagcgggct ggtcttcatg gtgctgcagg catggtgtgg cgcaccactg gcggcactgt
     4861 ttagcgtact tgtgctggtg ctgatgaccg ggggattcca tctggacggc ctcgctgata
     4921 cctgtgatgg cgtattttct gcacgtagcc gcgatcgcat gttggaaatc atgcgtgata
     4981 gccgtttagg cacccacggc ggtctggcat tgatttttgt ggtactggca aagattctgg
     5041 tgttaagcga gttggctctg cgtggcgaac cgatccttgc atcgctggcg gcagcatgtg
     5101 cggtcagccg cgggactgcc gcattattga tgtatcgtca tcgttatgcc cgggaagaag
     5161 ggcttggcaa tgtgttcatt ggcaaaattg atgggcgaca aacctgcgtc acactcggct
     5221 tagccgctat ttttgctgcc gtattattgc cgggtatgca tggtgtggct gctatggtgg
     5281 tgacgatggt ggcgattttc atcctcggtc agcttctcaa acgtacgctg ggcgggcaaa
     5341 ccggcgatac gctgggagct gctatagagc ttggtgaact ggtcttcctg ctggcactgc
     5401 tataaagaga cgtgacatgc aaacacttgc cgatttactg aatacgatcc ctgccatcga
     5461 tcctgccgcg atgtcgcgtg cacaacggca tattgacggg ttactcaaac ctgttggtag
     5521 cctgggaagg ctggaggcgc ttgccataca actggcagga atgccggggt tgaatggcat
     5581 accgcatgtg ggcaaaaaag cggtactggt tatgtgtgcc gatcacggcg tctgggagga
     5641 aggggtcgct atttccccaa aagaagtgac agccattcag gctgaaaata tgacccgcgg
     5701 aacaaccggc gtgtgtgtgc tggcagcaca agcgggcgct aacgtctacg taattgatgt
     5761 tggtattgat actgctgagc ctatccccgg gcttatcaac atgcgtgttg ctcgaggtag
     5821 tggcaatatt gcttcagctc cggcaatgag tcgccatcag gcagaaaagt tgcttttgga
     5881 cgtcatatgt tatacgcggg agctggcaaa aaacggtgtt acgctgtttg gtgtaggtga
     5941 actggggatg gcaaacacga caccggcagc ggcaatagtc agcacaatca ctggccgggc
     6001 tcctgaagaa gtggttggga ttggcgcaaa cctgccgaca gataaactgg ctaataaaat
     6061 tgatgttgtg cgccgggcga ttacgttgaa tcaaccaaat ccgcaggatg gcgttgatgt
     6121 cctggcaaaa gtgggtggat ttgatttggt cggaatggct ggcgtgatgt taggcgctgc
     6181 ttcctgcggt ttacccgtgt tgctggatgg atttctttct tatgctgccg cgctcgcagc
     6241 ctgccagatg tctcctgcaa tcaaaccgta tctcattcct tctcacctgt cggcagaaaa
     6301 aggggcgcgt attgcgctgt cgcatttggg gctggagcct tatctcaata tggagatgcg
     6361 tttaggtgag gggagtggcg cagctctggc gatgcccatc atcgaagctg cctgtgcgat
     6421 atataacaac atgggcgaac ttgctgccag taatattgtt ctaccgggga atacgacttc
     6481 tgatttgaac agttaaaaac gaaaagtctg ataaaagcta tacttaacgg atagctttcg
     6541 cgacttagga aagggacatg atgcgtcgtg taaatatttt ttgctcattt gctctgcttt
     6601 ttgccagcca tactagcctg gctgtaactt atccattacc tccagagggt agccgtttag
     6661 tggggcagtc gtttactgta actgttcctg atcacaatac ccagccgctg gagacttttg
     6721 ccgcacaata cgggcaaggg ttaagtaaca tgctggaagc gaacccgggc gctgatgttt
     6781 ttttgccgaa gtctggctcg caactcacca ttccgcagca actgattttg cccgccactg
     6841 ttcgtaaagg gattgtggtt aacgtcgctg agatgcgtct ttattactac ccaccagaca
     6901 gtaatactgt ggaagtcttt cctattggta tcggccaggc tgggcgagaa accccgcgta
     6961 actgggtgac taccgttgaa cgtaaacaag aagctccaac ctggacgcca acgccgaaca
     7021 ctcggcgcga atatgcgaaa cgaggggaga gtttgcccgc atttgttcct gcgggccccg
     7081 ataatcccat ggggctgtac gcgatttata ttggcaggtt gtatgccatc catggtacca
     7141 atgccaattt tggtattggg ctccgggtaa gtcagggctg tattcgtctg cgcaatgacg
     7201 atatcaaata tctgtttgat aatgttcctg ttgggacgcg tgtgcaaatt atcgaccagc
     7261 cagtaaaata caccactgaa ccagatggct cgaactggct ggaagttcat gagccgttgt
     7321 cgcgcaatcg tgcagaatat gagtctgacc gaaaagtgcc attgccggta accccatctt
     7381 tgcgggcgtt tatcagcggg caagaagttg atgtaaatcg cgcaaatgct gcgttgcaac
     7441 gtcgatcggg aatgcctgtg caaattagtt ctggttcaag acagatgttt taagagcgtt
     7501 ggtaattaga aagcaaaagc ctgctagaaa gcaggctttt ttgaatttgg ctcctctgac
     7561 tggactcgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
     7621 ggaatcgtgt gaacggggcg catattaatg gcagtgcatg gtgatgtcaa agggtgaaat
     7681 gcattttttt gtttgtttgc tgtcaatttc aacaaagtgg tgagctttca atcttattgg
     7741 gtaatgaacc atcgtggtgc ataccctcct tttatagggc aggggaacgc gacagctgat
     7801 taaaggagca aatgacgaat ctatcataat ctcttatttg acagttggtt agcttgtaca
     7861 tcaacgccaa aataaaactg gcaagcatct tgcaatctgg ttgtaagtaa tagcggcact
     7921 tgggccgatt cttaaaaacc ggaggcaaca tgaacttcag acgcctgaaa tacttcgtaa
     7981 aaattgtaga tattggtagc ctgacccagg ctgctgaagt attgcatatc gcacaaccag
     8041 cgctcagcca gcaggttgcc acactggaag gtgagttaaa tcaacaactt ttgatccgta
     8101 caaagcgggg cgttacgcca acagacgccg gaaaaattct ctatacccat gcgcgggcca
     8161 ttttacgtca gtgtgaacag gcccaactgg cggtgcataa cgttggtcag gcattatcgg
     8221 ggcaagtctc gattggcttt gcaccaggaa ccgctgcgtc atccatcacc atgcccttat
     8281 tacaggcggt tcgcgctgaa tttccggaga tcgttatcta tcttcatgaa aatagtggtg
     8341 cagtgcttaa cgaaaaattg ataaatcacc aactcgatat ggcggtgatt tatgagcatt
     8401 cccctgtggc tggtgtatcc agtcaggctt tgctgaaaga agatcttttt ctggtaggaa
     8461 ctcaagattg cccggggcaa agcgttgatg tgaatgctat tgcgaaaatg aacctctttc
     8521 tccccagtga ttacagtgct attagacttc gtgttgatga ggctttttcc ctacggcgac
     8581 tcacggcaaa agttattggt gaaattgagt ctattgccac gcttaccgca gcgattgcca
     8641 gcggcatggg cgttgcagta ttacccgaat cggccgcgcg ttcgttatgt ggcgcagtaa
     8701 atgggtggat gtcacgcatt accactcctt ccatgagtct ctctttgtca ttaaatttac
     8761 ccgccagagc gaacttatcg ccacaggcac aggcagtgaa agagttgtta atgtcagtga
     8821 ttagttctcc agtgatggaa aaaaggcagt ggcaattggt gagctaagtg ttatatcggg
     8881 atggaataag atgcgggttt ttattatttg ttatgccggg cattagactt taacaataac
     8941 gggaaatctg aactgcccgg agtttaccgt gaatttccaa caactaaaga taatccgcga
     9001 ggctgcacgt caggattaca acctgacaga ggttgcgaat atgcttttta cctcacagtc
     9061 aggcgtcagc cgtcatattc gggaactgga ggatgaactt ggcatcgaaa tatttgttcg
     9121 acgaggtaag cgactgctgg gcatgactga accgggcaaa gcattactgg tcattgcaga
     9181 acgtattctg aatgaagcca gtaatgttcg tcggcttgca gacctgttta ccaacgatac
     9241 gtctggcgtt ctcactattg caacgacgca tactcaggca cgttatagct tgccagaggt
     9301 cattaaagct tttcgcgaac ttttcccgga ggttcggctc gagctaatcc aggggacgcc
     9361 acaggaaatt gcgacattgt tgcaaaatgg cgaagctgat attggtatcg ccagcgagcg
     9421 tttgagtaat gacccgcagc tcgtcgcctt cccgtggttt cgttggcacc atagtttgct
     9481 tgttccacac gatcatccct tgacgcaaat ttcaccattg acgctggaat caatagcgaa
     9541 gtggccgtta atcacttacc gacaggggat tacggggcgc tcacgtattg atgacgcatt
     9601 tgcccgcaaa ggtttgctgg cagatattgt attaagtgcg caggattctg atgtcattaa
     9661 aacctatgtt gctcttgggc ttgggatcgg attagttgcc gaacaatcca gcggcgaaca
     9721 agaggaagag aatttaatcc gtctggatac gcggcatctt ttcgatgcta atactgtctg
     9781 gttgggactg aagcgaggac aacttcagcg taactatgtc tggcgctttc tggaactttg
     9841 taatgcagga ctgtcagttg aggatatcaa gcggcaggtg atggaaaaca gtgaagagga
     9901 aattgattat cagatatagc aaaaagcccg ctataaaagc gggcttttca gaaatttggc
     9961 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
    10021 aactacagag gaatcgctgt tgaggcacat cttaacggcg aaaaatcttt tgtcaaaccc
    10081 gatttttaaa ttccccaatt gtttgccgtt tctatcaaca gtttgttgca tttgcagaca
    10141 atttgttggc gaaaatcttt gcagctttgc tgattctccc tcattatttg aaatgtggtt
    10201 tcactttctg caattaaggt cggctttgaa tatctcctct gctttacgcc aggttgttca
    10261 cggcactcgc tggcacgcta aacgcaagag ctacaaagtg ttgttctggc gcgagataac
    10321 cccgcttgct gttcctatct tcatggagaa tgcctgtgtc ctgttgatgg gtgtcctgag
    10381 cacttttctg gtcagctggc tgggaaaaga tgcgatggcc ggcgtgggat tggcggacag
    10441 cttcaatatg gtcattatgg ctttttttgc agctatcgat cttggtacta ctgtcgttgt
    10501 ggcatttagt ctcggtaagc gggatcgacg acgagcgagg gtggcgacgc ggcagtcatt
    10561 ggtgatcatg acgttgtttg ccgtactgtt ggcaacgctt attcatcatt ttggcgaaca
    10621 aattattgat ttcgtcgcgg gtgatgccac gacagaagtt aaagcactgg cgttgactta
    10681 tctggagctg acggtactca gttatccagc agctgccatc actttgattg gtagcggggc
    10741 acttcgtggt gcagggaata cgaaaatacc gctactgatt aacggtagcc tgaatattct
    10801 taatattatt attagcggca tattgattta cggccttttc tcctggccgg gactgggatt
    10861 tgtcggggct gggttgggtt taaccatttc tcgttatatt ggcgcagttg caattttgtg
    10921 ggtgctggcg attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc
    10981 gctgaatttt agcattatct gggaagtcat ggggattggt attccagcga gtgtcgaatc
    11041 agtgttattt accagtggtc ggttattaac ccaaatgttc gttgccggga tggggaccag
    11101 tgttattgcc ggaaatttta tcgcgttttc aattgcggct cttatcaact tacccggaag
    11161 tgcgctcggc tctgcttcta cgatcattac aggccgaagg ttgggggtag ggcagatagc
    11221 gcaagcagag attcagttgc ggcatgtgtt ctggctgtcc actcttggat taacggccat
    11281 cgcctggcta acggctccct ttgccgggct tatggcatcg ttttacaccc aggatccaca
    11341 ggttaaacat gtcgttgtga ttctgatttg gctaaatgct ttatttatgc ctatttggtc
    11401 cgcctcatgg gtgctacccg ctggatttaa aggtgctcgt gatgcccgtt acgccatgtg
    11461 ggtttcgatg ttgagcatgt ggggttgccg ggttgtcgtc ggttatgtgc tgggcatcat
    11521 gcttggctgg ggtgtggttg gtgtctggat gggaatgttt gccgactggg ctgtgcgggc
    11581 cgtgctgttt tactggcgaa tggttactgg acgttggcta tggaaatacc ctcgatccga
    11641 gccgcaaaag tgtgaaaaaa agccagctgt gtcggaataa acgacaaaat gcagattatt
    11701 tcagcaaacg atttcaaatt taaaaaacag gctttgacat tatgggtggg catcgctaat
    11761 attcgcctcg ttctcacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
    11821 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ttagttttga gacatctcta
    11881 tgagtctcaa aacagttaaa aaataaaaat tatttttacc cggttacctt gtaaaaaagt
    11941 gaccgggttt tttgatttct gtgtttcgtg cgcttaatcg agtctcacgc ttcagtgagc
    12001 aagttgcccc caacagacat cacgattcgc aaggtcaaaa actctgttcc atgaagtacc
    12061 gtacaaagat aaagagaaaa atgccttatg cgggatgtcg ctatcgcgtc tttatcggga
    12121 aactatgcgt tattgccact ttgccttctg taatcagaaa aagaagaact ggaattttaa
    12181 taaatattat ttctctggga acggggattt tttataatca
//
LOCUS       DAZGCS010000128        12160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGCS010000128 DAZGCS010000000
VERSION     DAZGCS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.130"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..936)
                     /gene="yihR"
                     /locus_tag="ACHK01_004160"
     CDS             complement(34..936)
                     /gene="yihR"
                     /locus_tag="ACHK01_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO8099013.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1074..2315)
                     /gene="yihS"
                     /locus_tag="ACHK01_004161"
     CDS             complement(1074..2315)
                     /gene="yihS"
                     /locus_tag="ACHK01_004161"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO8099014.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2332..3210)
                     /gene="yihT"
                     /locus_tag="ACHK01_004162"
     CDS             complement(2332..3210)
                     /gene="yihT"
                     /locus_tag="ACHK01_004162"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO8099015.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3234..4130)
                     /gene="yihU"
                     /locus_tag="ACHK01_004163"
     CDS             complement(3234..4130)
                     /gene="yihU"
                     /locus_tag="ACHK01_004163"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO8099016.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4298..5194
                     /gene="yihV"
                     /locus_tag="ACHK01_004164"
     CDS             4298..5194
                     /gene="yihV"
                     /locus_tag="ACHK01_004164"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO8099017.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5228..6013
                     /gene="csqR"
                     /locus_tag="ACHK01_004165"
     CDS             5228..6013
                     /gene="csqR"
                     /locus_tag="ACHK01_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8099018.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6112..6711
                     /gene="yihX"
                     /locus_tag="ACHK01_004166"
     CDS             6112..6711
                     /gene="yihX"
                     /locus_tag="ACHK01_004166"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO8099019.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6705..7577
                     /gene="yihY"
                     /locus_tag="ACHK01_004167"
     CDS             6705..7577
                     /gene="yihY"
                     /locus_tag="ACHK01_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO8099020.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7574..8011
                     /gene="dtd"
                     /locus_tag="ACHK01_004168"
     CDS             7574..8011
                     /gene="dtd"
                     /locus_tag="ACHK01_004168"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO8099021.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8056..8997
                     /gene="fabY"
                     /locus_tag="ACHK01_004169"
                     /gene_synonym="yiiD"
     CDS             8056..8997
                     /gene="fabY"
                     /locus_tag="ACHK01_004169"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO8099022.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAIADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9061..9969)
                     /locus_tag="ACHK01_004170"
     CDS             complement(9061..9969)
                     /locus_tag="ACHK01_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8099023.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10198..10509
                     /locus_tag="ACHK01_004171"
     CDS             10198..10509
                     /locus_tag="ACHK01_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8099024.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10510..10800
                     /gene="nadS"
                     /locus_tag="ACHK01_004172"
     CDS             10510..10800
                     /gene="nadS"
                     /locus_tag="ACHK01_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8099025.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11386..11604
                     /gene="yiiE"
                     /locus_tag="ACHK01_004173"
     CDS             11386..11604
                     /gene="yiiE"
                     /locus_tag="ACHK01_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO8099026.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            11823..12065
                     /gene="yiiF"
                     /locus_tag="ACHK01_004174"
     CDS             11823..12065
                     /gene="yiiF"
                     /locus_tag="ACHK01_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO8099027.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT         2978 a         2948 c         3201 g         3033 t
ORIGIN      
        1 tcttcaatga ttttgactgc taatcaatac aagttatttt ctctgtccgt aaatgttaaa
       61 aaacaggcgg tgcggttttc ctggttccag taataataag tcgatacctg aattaaaggc
      121 gttaggcggg caactcatag gttcaactgc cagtccctga cgctgtaatt tttcaccgct
      181 ataaatttgt acccagggtt gatcgcaaca aaggctaacc gaaagcgcct gttgtggatg
      241 ggtaatggtt atttcccaca gatcattagc ggttttaaag gtgtggtcga tttttgtcgc
      301 ggctattgtt ttcgcctgcg tgaaatttaa atccagctca tcaacgtggt gaagcgttgt
      361 cggatttacg tgttcatcaa cggcataaac ctggtttgcc ggaagctgga acagatattc
      421 atcaacagat gttaagttgc aggttaaata agtatgaata cccacgccat aaggcgctgc
      481 gacagtgccg atattctggc ttgcgatctc aacggaaaga cccgtgtgtg cgttgagcga
      541 ataaacaact tgtgaagcca gcataaacgg atagccgtaa ctgggcggca gaaaagctgt
      601 cagggtaacg ctggtggcgg ttaattcact aatttgccag tctcgccagg caagcagccc
      661 gtgaatagcg gcttttgaac tatgttcatt aatgggtaac tgatattcct gcccctgata
      721 gcggtaacag ccgttggcga tccggtttgg ccacggaata agcactttac ccagatgcgc
      781 cagcggcatc tcttcgggtt tgtgcgggat gactaaatga caaccctgaa aggttaattc
      841 agccagacca gcaccgacgg tcacaatggt cgcgtgataa tcccccgcgg caagtgaaac
      901 ggtctgtcca ctgcaatgca tattggtgat ttgcatacaa ggcaccttaa ttaacgacat
      961 acagcctccc gtgaggctga tgtcaaaccc aaaaactgca ccggacggtc ccctcgcccc
     1021 tttggggaga gggttagggt gaggggaaaa cccgcaccct attttcagat cagttatttc
     1081 gcattaatat ccagcagacc cgctgcaacc gctggagcca tgccgggcgc taacgggata
     1141 cgcgggatca ccaggcaatg cagcaggtga taaatatcct gtttgccgtc ccagactttg
     1201 gtggtgacct tattgtccgc atccagctcc tgccaccagg aaccattttc atagtccatc
     1261 aggtacttaa tgcagtactc ccaccatgtt tgataccagg tttcatactg gcgatcgcca
     1321 gtgacggtgt agagcgcgta ggccgtaccc attgcttcga cgataggcca acgtacacgt
     1381 tcgcggacca ccggttttcc ttcccagtca acggtataaa caatcccgtc cgcgccatcg
     1441 ggtgcccagg catcgcgcac ggtggcgtta aacagacctt tggcatcttc tagcagccat
     1501 gctggtggtt gttcgcaacg ggcttccagg gcagcatgga tgtgcagcat taaacggccc
     1561 cattcgatcc agtggcctgg tgtacctcca aacgcgcgga agcgatgcgc cgggttatct
     1621 ttgttgtaat ccggcagcgg attccactgg gtatcgaagt gttcgttaac gcgataatga
     1681 ttatttctgg cgacgtcgtg gataatcacg gaagccacgc gaatcgcgcg atccagccat
     1741 tttttgtcgt gagtgacgtc ataaacaatc aagaaagctt ccaccgcgtg catattggca
     1801 ttgccgccgc ggtactcttc ggttttgctg aaggcttcgt cccaggattc caggcacatc
     1861 tgctcttctt cgctccagaa atatttctcg ataatttcaa tggtgtaatc gagcagcttg
     1921 cgcgcttccg ggtgacccgt tgtgacggcg ctggcggcac ccagcagagc aaagaagtgt
     1981 tgatagccct gtttggaggc atccaccacg ccctcgtcat tcacgcaggc ataccagccg
     2041 ccatattttt tatcacgcag tgcgccgttc atggctttga taccgtgatc aaccaacgag
     2101 taagcgccag gtcgacccat cgccgcagca acggaataaa cgtgcaacat acgagcggtg
     2161 atccacagat gggtgcccat ctcttctttg atttgccctt tattgcctaa ccagccaaaa
     2221 ccagtcggca ctacggaatt tttaccaaaa tcaaagatgc ggtcggtttc ctgttccagc
     2281 caacggttgt ggcttagggt gttaaaccat ttcattcctt ttatcctcat cttagcggcg
     2341 tttagccatc atttcgtcga cgatatcgcc aagttgttgt aatttcggtg cgcaaacgtc
     2401 acgcagcatc agctcgttgt ctggtaaacc gacgaccgat gcccagactg cacggcctgc
     2461 caggaatccc gatgcgcccg ccgtcattgc cacgcgtacg gcacgcggga acagtttttc
     2521 gtcgacaccg gaagagagga tcacccatgg catattgata tggtcattca gacgttgtga
     2581 agcacagaga agctcttgtt gcggaccttt gccataaagc ggcatttcaa ctttgtagag
     2641 gtcagcgcca ctgtcgccca gctctttggc ggcatcgatg atcgcttgtt cgcgatcgaa
     2701 tttatcgcca cgacgcggtg ggcggacgac tggctcaatg atgcttacca gaccgtgtga
     2761 gtggcacagt tcgttgaact ctttcaccat atccagacgt tgctgcgcat cttcatcact
     2821 acgccacagc accagcagtt ttaaggcttt gccaccgtct tgtttgatct gtagcggatt
     2881 gattttgcgg tcaataacca cgctatcgac cggaataccg ttgccaggaa tgaactcatc
     2941 ggcggcgaca atcatggcgc aacttttggc aatcgcgttt tgctcaacca cctggcggta
     3001 gcagaattgt tgatctacca gaatcgccga ggcataaggc gagagggcct ttgcagcgtt
     3061 aactttgaaa tcagttaaaa cgctatcggc taccggagcg ggtgccccag ccgcggcaaa
     3121 catcatgcgc atggcttcgc gctgatcgac cgccagcatg gcaaaaccgc ccgatgcgcg
     3181 cgtaatgtcg ttgatggtgt acttattcat tcatttattc ctttatccag tcgttacatt
     3241 tttactttgg cagtcatccc ggcactgaca cggacctgtt ccagaatggc ggaccagtcc
     3301 tggcgaccgc gacccgctgc gcgcgcctgg ctataaacct cccgtgaggc ggcccccagc
     3361 ggcattggca catgcagctg gttggcgaca tcaagggcga tgccaagatc cttatgggca
     3421 agatcgatca tgaaggcggg agaaagatct ccgctgagga ctttgtttgg ccaggaagtg
     3481 gtgaagtgac ctttaccggc ggcggtaccg ctcatcactt tgacggcaac atcgaaggga
     3541 agattcaggg cttcgcacaa aacggcggct tctgccgaaa gcgcattgag cgcgatgctc
     3601 atgtagttgt tgatgagctt aacgcggatc cccatgcccg gaccgccaga gttgatcaac
     3661 tcactgccca tcgccatcag gatcggtgtg gcacgttcaa cttgttcagc ggtgccgcca
     3721 gccagcagta acagagtacc ggtaatggca tttgcagaag tacggcctac cggaacatcc
     3781 atcatgctga agcctttggc ttgcatatcg gcaatcaatt tatcggtttg cagcggatgg
     3841 atggtggaca tatcaatgac cagcgcatcg gtagataagc cttcgcaaac gccgttttca
     3901 ccgaacaaca cgttgcgcac cagatcgcca ttcggcaaca tggtaatgat aaattcggca
     3961 tctttagcgg cctgcgccgg gttggcggcg ggagtcgcac ctttgtctac cagatgccgc
     4021 acagcttcgg cgttcacatc aaagacgcga agttgatgcc cttgctgcaa taaattgctc
     4081 gccattggcg aacccatttg tcctaaaccg ataaacgcga ttgctgccat aaccctctcc
     4141 tgaatacagt gatgtcactt tttgtcattt atgacatgct ttgcttgtct gtttttgatc
     4201 gtatttgtaa tttatcgtca aaaaattgac agccgtcact ttttaaacaa ttggtgaaat
     4261 taaaatgaac gcatcccaaa atgtttaagg aatgaccatg attcgtgttg cttgtgtagg
     4321 tataaccgtg atggatcgca tctattacgt ggaagggtta ccgacggaga gcggtaaata
     4381 cgtggcgaga aattatacgg aagttggtgg cgggccagcg gcgacggcag cggttgcggc
     4441 ggcaaggctg ggggcgcagg tcgattttat tggtcgcgta ggtgatgacg acacgggcaa
     4501 cagcctgctg gcagagctgg aatcctgggg cgttaacacc cgttacacca aacggtataa
     4561 ccaggcgaaa tcttcgcaat ccgccatcat ggtggatacc aaaggcgagc ggataatcat
     4621 taactacccc agcccggatc tgctgcctga cgcagagtgg ttggaggaaa ttgatttctc
     4681 tcagtgggat gttgtgctgg cagatgtacg ctggcacgac ggcgctaaaa aagccttcac
     4741 cctggcccgt caggcgggtg tgatgaccgt tctggacggg gacattacgc cgcaggatat
     4801 cagtgagctg gtggcattaa gcgatcacgc ggccttttca gaaccgggtc tggcgcgctt
     4861 aacgggcgtg aaagagatgg ccagtgcgct aaaacaggca caaacgctca caaatggtca
     4921 tgtctatgtg acccagggta gcgcaggctg cgactggctg gaaaatggtg ggcgtcagca
     4981 tcaaccggcc ttcaaagttg atgtggtaga taccacaggt gcgggtgatg tttttcacgg
     5041 cgctttggcg gtggcgctgg caacaagtgg ggatttagcg gagtcagtcc gcttcgccag
     5101 cggtgtagcg gcgttaaaat gcacacgtcc cggtggacga gccgggatcc ctgactgtga
     5161 tcaaacccga tcttttttgt cactttttgt ataaaatgcc agggtgatgg tttttcgagg
     5221 aattttcatg agccttaccg aactaaccgg taacccgcgg cacgaccaac tcctcatgct
     5281 gatcgccgag cgtgggtata tgaatattga tgagctggca aatctgctgg atgtctccac
     5341 gcagacggtc cgccgggata ttcgtaaatt aagcgagcaa ggcctgatta cgcgccatca
     5401 cggtggcgcg ggtcgggctt ccagcgtcgt taatacggcg ttcgagcagc gtgaggtttc
     5461 gcaaaccgag gaaaaaaaag cgattgccga agcggtggca gactatattc ctgatggttc
     5521 aacaatattt atcaccattg gtacgactgt tgagcatgtt gcccgggcgt tacttaacca
     5581 taatcatttg cggataatca ccaacagcct gcgtgtggcg catattcttt accacaaccc
     5641 gcgctttgaa gtgatggtgc ccggcggtac gttgcgctct cataatagcg ggatcattgg
     5701 cccttcagcg gcgtcctttg tggctgattt tcgtgctgat tatctggtaa caagcgttgg
     5761 ggcgattgag agcgatggcg cgttgatgga gtttgatgta aacgaagcta acgtggtgaa
     5821 aacgatgatg gcgcacgcga gaaatattct gctggtcgcc gatcacacta agtatcatgc
     5881 ttcggcggcg gttgaaattg gtaacgtggc acaggtcact gcgctcttta ccgacgagct
     5941 gccgcccgct gcgctaaaat cacgcttaca agacagccaa attgaaatca ttcttcccca
     6001 ggaagacgcg tagatttttg tgaccgttaa cctggcttca taccttgcca catagccaaa
     6061 cccatccttt cccgctacag ttaatttctt gtggcgcgaa aggaggcaaa aatgctctat
     6121 atctttgatt taggtaatgt gattgtcgat atcgacttta accgtgtgct gggagcctgg
     6181 agcgatttaa cgcgtattcc gctggcatcg cttaagaaga gttttcatat gggggaggcg
     6241 tttcatcagc atgagcgtgg ggaaattagc gacgaagcgt tcgcagaggc gctgtgtcat
     6301 gagatggctc taccgctaag ctacgagcag ttctctcacg gctggcaggc ggtgtttgtt
     6361 gcgctgcgcc cggaagtgat cgccatcatg cataaactgc gtgagcaggg gcatcgcgtg
     6421 gtggtgcttt ccaataccaa ccgcctgcat accaccttct ggccggaaga atacccggaa
     6481 attcgtgatg ctgctgacca tatctatctg tcgcaagatc tggggatgcg caaacctgaa
     6541 gcacgaattt accagcatgt tttgcaggcg gaaggttttt cacccagcga tacggtcttt
     6601 ttcgacgata acgccgataa tatagaagga gccaatcagc tgggcattac cagtattctg
     6661 gtgaaagata aaaccaccat cccggactat ttcgcgaagg tgttatgcta aaaaccattc
     6721 aggacaaagc caggcaccgt actcgtccac tatgggcctg gctaaaacta ctctggcaac
     6781 gtattgatga ggacaacatg acaaccctgg caggtaacct tgcctatgtg tctttgctct
     6841 cattagtgcc gctggttgcc gttgtttttg cgcttttcgc cgcttttccc atgttttccg
     6901 acgtcagcat tcagttgcgt cactttattt ttgccaactt tctgcctgct actggcgatg
     6961 ttattcagcg gtatatcgaa caatttgttg ccaattccaa caagatgacc gccgttggcg
     7021 cgtgcgggct gatcgtcacc gcattattgt tgatgtactc catcgatagc gcgttgaata
     7081 ccatctggcg cagtaaacga gcgcgaccaa aaatttactc gtttgccgtg tactggatga
     7141 ttttaacgct ggggccgctg ctggcagggg ccagtctggc gatcagttcc tatttgctct
     7201 ccctgcgctg ggcgagcgat ctcaatactg tcatcgacaa cgtgctgcgt atttttccgc
     7261 tgctgttgtc gtggatctcc ttctggttgc tgtacagcat tgttcctacc atccgcgtac
     7321 ctaaccgcga cgcgattgtc ggcgcgtttg tcgctgcact cctgttcgaa gcaggaaaga
     7381 aaggtttcgc gctttatatc accatgttcc cgtcatatca gctcatttac ggcgtgctgg
     7441 cggtgatccc cattctcttt gtttgggtct actggacatg gtgtatcgtc ttgcttggcg
     7501 cggaaattac tgtcactctc ggggaatacc gcaaactaaa acaagcagct gaacaagaag
     7561 aagacgacga accatgattg cattaattca acgcgtaacc cgtgccagcg tcaccgtgga
     7621 gggagaagtg acgggcgaaa ttggcgcggg acttttggtg ttattgggtg tcgaaaagga
     7681 tgacgacgaa cagaaagcaa accgtctgtg cgagcgtgtg ctcggctacc gcatctttag
     7741 cgatgccgaa ggcaagatga atctcaacgt gcaacaggcg ggcggcagtg tactggtggt
     7801 ttcccagttt accctcgccg cagataccga acgagggatg cgcccaagtt tctccaaagg
     7861 cgcatcaccg gatcgcgcag aggcgttata tgactatttc gtcgaacgct gccgccagca
     7921 agagatgaac acgcaaacag gacgcttcgc tgccgatatg caggtatcgc tggtcaatga
     7981 tggccccgtg acattctggt tgcaggtatg agccagcttc cagggttgtc acgggaaaca
     8041 agagagagta tcgctatgta tcaccttcgg gttccacaaa cagaagaaga attagagcgt
     8101 tactatcagt ttcgctggga aatgttgcgt aagcccctgc atcaaccaaa aggttcggaa
     8161 cgcgacgcgt gggatgcgat ggcgcatcac cagatggtcg tcgacgagca gggtaatctg
     8221 gtggcggtag gccgattgta tattaatgcc gacaatgaag cgtccattcg ctttatggcc
     8281 gttcatcccg acgtgcagga caaagggtta ggaacgctga tggcgatgac cctggagtcg
     8341 gtggcgcgtc aggaaggcgt taagcgcgtg acctgtagcg cccgtgaaga cgcggtggag
     8401 tttttcgcca agctgggatt cattaatcag ggggagatca ccaccccaac taccacgccg
     8461 attcgccatt ttttgatgat taaacccgtt gccactctgg atgatattct gcatcgcggt
     8521 gactggtgcg cccagctgca acaggcgtgg tatgaacaca ttccattaag cgaaaaaatg
     8581 ggcgtgcgca ttcaacaata taccgggcaa aaatttatca ccaccatgcc ggaaaccggc
     8641 aatcagaatc cgcaccatac gctgtttgcc gggagtttat tctcgctggc gacgctcacc
     8701 ggttgggggc ttatctggct gatgctgcgc gaacgccatc tcggcggaac gattattctg
     8761 gcggatgcgc atatccgcta cagcaagccg attagcggta aacctcatgc gatagccgac
     8821 cttggtgcct taagcggcga tctcgaccgt ctggcgcgcg gacgaaaagc acgggtgcag
     8881 atgcaagttg aaatctttgg cgacgagacg ccgggtgcag tgtttgaagg cacgtatatc
     8941 gttctgcccg cgaagccatt tggcccgtat gaagagggcg ggaacgaaga agagtagggg
     9001 cgttttactt gactgaaaag gttttcaatc tgaaaacctt ttcagggccc aggtccgaag
     9061 ttaccttccc gctctccagg caacatcccg tagtgccgat cgcgcggcct cgctgatacc
     9121 acctaactga aaaaatccgt ggataacgcc aaggtagcgt tggcaggtgc attgcactcc
     9181 ttgccctgtc atgcggtgat acaacgcttc accttcatcg caaagtgggt cgtattcagc
     9241 ggtaatgata tgtacaggtg gcaagccatt gaaatcatcg cgccaaagag ggctggcttc
     9301 aggatgcctg cgttcaatgc ctggcagata catctcaaag ccgctcagta aagtgtcgcg
     9361 tgtgatgatg taatcgtgac cattgcgtat gtagctttcg aaatgcgcag tcgcatcaag
     9421 cataggataa attaagatga gttgtgccgg ttgccactcg ccagcacgct ttaatcgcaa
     9481 agcagttacc agcgccagat gtccgcccgc actgtcaccg gcaagagtta tacgttgttt
     9541 atcaacgccg agtcgttcgg cgtgttgcca gactatttct gcacctcgtt gcgcatcatc
     9601 atgggcggcg gggaaagtat gttccggtgc cagccgatac tggacggcaa taacccgaca
     9661 ttgcccgtaa taggctaact ggcgcagctg gttgtcgtga gttgcgaacc cgccgctaac
     9721 aaaacagcca ccgtagtaat aaattatcgt cgggagtaat gtcggggcat tcagcggcga
     9781 cactattcgt aaggtcatcc cttcaagttc gatagtctga atatctaccc gcgtttctgt
     9841 ctcacccgca aggacagcgc tggcgatata gccctccctg cgctgcgcaa tactttgttg
     9901 acgcgatgag gggcgacccg cagcgataaa cttttcgact aactctgcaa ttcctttttc
     9961 cagtgccata gtagaactta ttttgctgta tgaatgtaca gtcttataca cccgtttttg
    10021 ctggatggat acagggaaat tcacgggcgt gaaaaatctg gaaagcgcct cgcaaatcta
    10081 aagacaaacc cggcagagac gtggttttgc tggagcacta tcagtaagct agtggtttca
    10141 agggtacgtc aatgacgtac aaaaaggatt gttactctaa gccaatggcg tataattatg
    10201 ctcttcatcg aaacggaaat ttttactgaa gatgttcaaa aactgctgac cgacgatgaa
    10261 ttcagtcgtt ttcagttttt cctggccttg aatcctgact atggtgaggt aatacctgaa
    10321 actggtgggt taagaaaggt tcgatgggtt tcaggaggaa aggggaaacg tgctggtgtc
    10381 agggttattt actttcatca ggttaaacat tatgagatca gattactgct tatttatcgt
    10441 aagggtatta aagatgatct gtctccccag gaaaaagcaa tgctccggtt gttaaatacg
    10501 aggtggtaaa tggataaggc actttttgag cggttgactc acagcatggc ccagatgaat
    10561 gaaattattg aaggaacgcg acagccttcg cggacttttg aagttgatgc tatgaaaatc
    10621 aaagaaatac gccgcgcttc agggttgtca caatctaaat ttgccgatct tatttctgta
    10681 agcgtagaca cgttgcgtaa ctgggagcag gggcgacgtt caccgaccgg gcctgcaaag
    10741 gctttgctgc gagcgattgc caatgatccg caacatgtgc tgcaagcact taaccgttag
    10801 aggcatggtt atgccataca gaaagcacta gcattcagaa ctcctgtgaa agcggcatag
    10861 tggtcgcctg cccaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat
    10921 gatgcgatta aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgata
    10981 ccggggggtt ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga
    11041 tgaacaagta aagaccggtt ggtggcagta ctgaccggtc tgtgatggat gattaatttc
    11101 accttaaatc ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg
    11161 caaataaata tctctgaata taatatattc atagcaccca taattcatcc tgggcgataa
    11221 ctaaaccatt tttgagtaat aaatgaaaca actgcaacac tcttattatt tctggaaaac
    11281 gcctcgcaaa aacaaaacaa catgcaaaaa tccgcttttt ttaaatgatt ttccggcgta
    11341 attttcgcac atacattaat gagtaatata ggtaaggtgg tggtaatggc gatgaatacg
    11401 gtttttcttc atttatcaga agaggcaatt aaacggctga acaaacttcg gggctggcgt
    11461 aaggtttcac gatctgcaat tttacgcgaa gcggttgagc aatatctgga gcggcagcaa
    11521 tttccggtgc gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg tgttgatgat
    11581 cagtgtaagg agcataagga atgaaaaaaa tttttcattc ttgaatataa aaaaacagat
    11641 gcccttattc tggtattaat acaaggctgt tttacttgaa cttataataa ctgcaactgt
    11701 tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt
    11761 taaagattcg gcaagggtac ttttctctcc atagattcaa aaatgatatt ggcgagatat
    11821 ttatgaactc tctggcaggt atagatatgg gcagaatttt actcgattta tcgaatgagg
    11881 tgattaagca acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa
    11941 gggaagcggt agatcaatac ctgataaatc aatcgcaaac agcaagaacc agtgttcctg
    12001 gcatctggca agggtgtgag gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat
    12061 ggtaatggga aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg
    12121 atgcggcgtg agcgccttat caggcctaca aagtcatgca
//
LOCUS       DAZGCS010000129        12156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGCS010000129 DAZGCS010000000
VERSION     DAZGCS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.131"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(111..866)
                     /gene="mlaA"
                     /locus_tag="ACHK01_004175"
     CDS             complement(111..866)
                     /gene="mlaA"
                     /locus_tag="ACHK01_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO8099028.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1160..2092
                     /locus_tag="ACHK01_004176"
     CDS             1160..2092
                     /locus_tag="ACHK01_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO8099029.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2168..2242
                     /locus_tag="ACHK01_004177"
     tRNA            2168..2242
                     /locus_tag="ACHK01_004177"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2201..2203,aa:Arg,seq:cct)
     gene            complement(2684..3931)
                     /locus_tag="ACHK01_004178"
     CDS             complement(2684..3931)
                     /locus_tag="ACHK01_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO8099030.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4003..4917)
                     /locus_tag="ACHK01_004179"
     CDS             complement(4003..4917)
                     /locus_tag="ACHK01_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO8099031.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5133..6566
                     /gene="cscA"
                     /locus_tag="ACHK01_004180"
     CDS             5133..6566
                     /gene="cscA"
                     /locus_tag="ACHK01_004180"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO8099032.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6574..7569)
                     /locus_tag="ACHK01_004181"
     CDS             complement(6574..7569)
                     /locus_tag="ACHK01_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8099033.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7812..8030
                     /locus_tag="ACHK01_004182"
                     /pseudo
     CDS             7812..8030
                     /locus_tag="ACHK01_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8048..9376
                     /gene="dsdA"
                     /locus_tag="ACHK01_004183"
     CDS             8048..9376
                     /gene="dsdA"
                     /locus_tag="ACHK01_004183"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO8099034.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9484..11022)
                     /gene="emrY"
                     /locus_tag="ACHK01_004184"
     CDS             complement(9484..11022)
                     /gene="emrY"
                     /locus_tag="ACHK01_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO8099035.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11022..>12156)
                     /gene="emrK"
                     /locus_tag="ACHK01_004185"
     CDS             complement(11022..>12156)
                     /gene="emrK"
                     /locus_tag="ACHK01_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO8099036.1"
                     /translation="SNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNA
                     DPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLY
                     LQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAA
                     IQAYKANKALVMNTPLNRQPQVVEAADATKETWLALKRTDIRSPVTGYIAQRSVQVGE
                     TVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGIN
                     MGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNED
                     IAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3401 a         2963 c         2780 g         3012 t
ORIGIN      
        1 tttacatcat tacgtccggg tcattgtcgg tttatctcct tttacttgtg gtaattgagg
       61 gataacaaca aaaaaggtga gtattgcgac tcaccttttt tatttgtttc ttattcagaa
      121 tcgatatctt ttaaatcatc ctgaatcgct tgcgcgttcg gattttcctg cggtttgagt
      181 tcgccgccat tagcgatgaa atcatgacgc tggaagtacg cttcgcgcac cataatataa
      241 ggatcggacg actgacgcag cagaccatcg gaatccaaca actgcgcacg agtttctatc
      301 ccttcaagcg tccatttacc cacagacatc ggccaggtca gccaggaaag aaccgggtaa
      361 agaccatccg ccatatcacc accgtcatca cgcagcgtga agctaccgta gaacggtaac
      421 tggacgtaag gcccataacc cacgccataa tgaccaagcg tactaccgaa gcggtgaggt
      481 tcagtccgtt gcagtttcgg gttcgccatc cctgcaacat caataaaacc gcccatcccc
      541 aaaatggtgt tcaggaaaaa gcgggtaaag tggaccatcc cctgataagg gtcgccctgc
      601 aagaagtagt taaccatcac cgcaggttct tcaaggttgc cagtaaagtt gctcaaaccg
      661 ttacgcgccg gttgcggaac ataatcacgc caggcgacag cgaccggtcg aacaatatac
      721 gggtctaata cattgaagtt gaagttgtac atggtgcggt tgaacccttc taacgggtca
      781 gaacgccctt gctgatctgt accggaactc gcacacccca ccagaagcgt agttcccaga
      841 gcaagcgccg acaggcgaag cttcataaat gtctccctgt ttttttatgg cttatgcagt
      901 ttgccatcca tgacggaacg ataccgtatc cgcctgttta ggtgtgggcg attgtaacag
      961 cacgtcaact gatgtccaga cgccctgatt tgctgatttg atcatagcct ggtaatcgcc
     1021 gccctgtagg ctacttgatt ctatagaaac agaaaaaggc aaacgcagcc ttttctacat
     1081 tttcagagta actcccgcca gttgcgagca aaaaagccgc tacgctttag ctatacgtgc
     1141 taattcaaga gaagagacca tggacaacga caaaattgat caacacagcg acgaaattga
     1201 agttgagagc gaagaaaaag agcgcggcaa aaaaatagaa atagatgaag accgactccc
     1261 ctcccgggcg atggcaattc atgagcatat ccgccaggat ggtgaaaaag agctggaacg
     1321 cgacgcaatg gcgctactgt ggtcagccat tgcggcgggt ctgtcgatgg gcgcttcgct
     1381 actggcaaaa gggatatttc atgtcgaact ggaaggagtg ccaggcagct tcttactgga
     1441 gaatctcggt tatacctttg gttttattat cgtcattatg gcccgccagc aattatttac
     1501 cgaaaacacc gtgactgcgg tactacccgt catgcaaaaa ccgacaatga gcaacgtcgg
     1561 cttacttatg cgattatggg gcgtcgtgct gctgggtaat attctcggga caggtattgc
     1621 tgcatgggca tttgaatata tgcctatctt caatgaagaa actcgcgatg catttgtcaa
     1681 aatcggcatg gatgtgatga agaacacccc cagcgagatg tttgccaacg cgatcatttc
     1741 cggctggctg atcgccacta tggtttggat gtttcctgct gcgggtgcgg caaagattgt
     1801 ggtgattata ttgatgacct ggcttattgc actgggtgac accacccaca tcgttgtcgg
     1861 ttctgttgaa atcctctatc tggtgtttaa cggcacgctg cactggagcg atttcatctg
     1921 gcccttcgca ctacctactt tagcggggaa catctgcggc ggcaccttta tcttcgcgtt
     1981 aatgagtcat gcacagattc gtaacgacat gagcaacaag cgtaaagcag aagcacgcca
     2041 aaaagcagaa cgtgcggaaa acattaagaa aaatgataaa aacccagcat aaatggcgag
     2101 ggtttaagca atcgagcggc agcgtactta ccccgcagtc cattagcggg tatactcatg
     2161 ccgcattgtc ctcttagtta aatggatata acgagcccct cctaagggct aattgcaggt
     2221 tcgattcctg caggggacac cagataccct tcaaacgaaa tctaccttca ccccgtaaaa
     2281 gatgggtttg gcagcacact tgccttatat ctactcattt ttactgcaac aggttgaaat
     2341 ctcagcactg tcagaaagcg ctgatgacta aacagccctg ggccgggcga tgtaaccatc
     2401 acacagaatc ctgatagcga aatatggcgt gactcgatac ttcactccgc aatgcattcc
     2461 ttgatgaatt cgcaggaccg tgatacacgg gacaggtcac tgaatgacga caatgtcctg
     2521 gaaatcagcg aaccgcgcat ctgaagtaca tttgagcgac tgtaccagaa catgaatgag
     2581 gcgtttggat taggcgatta ttagcagggc taagcatttt actattatta ttttccggtt
     2641 gagggatata gagctatcga caacaaccgg aaaaagttta cgtctatatt gctgaaggta
     2701 caggcgtttc cataactatt tgctcgcgtt ttttactcaa gaagaaaatg ccaaatagca
     2761 acatcaggca gacaataccc gaaattgcga agaaaactgt ctggtagcct gcgtggtcaa
     2821 agagtatccc agtcggcgtt gaaagcagca caatcccaag cgaactggca atttgaaaac
     2881 caatcagaaa gatcgtcgac gacaggcgct tatcaaagtt tgccacgctg tatttgaaga
     2941 cggatatgac acaaagtgga acctcaatgg catgtaacaa cttcactaat gaaataatcc
     3001 aggggttaac gaacagcgcg caggaaagga tacgcaacgc cataatcaca actccgataa
     3061 gtaatgcatt ttttggccct acccgattca caaagaaagg aataatcgcc atgcacagcg
     3121 cttcgagtac cacctggaat gagttgagat aaccatacag gcgcgttcct acatcgtgtg
     3181 attcgaataa acctgcataa aagacaggaa aaagttgttg atcaaaaatg ttatagaaag
     3241 accacgtccc cacaataaat atgacgaaaa cccagaagtt tcgatccttg aaaactgcga
     3301 taaaatcctc tttttttacc cctcccgcat ctgccgctac gcactggtga tccttatctt
     3361 taaaacgcat gttgatcatc ataaatacag cgccaaatag cgagaccaac cagaagttga
     3421 tatggggact gatactaaaa aatatgccgg caaagaacgc gccaatagca tagccaaaag
     3481 atccccaggc gcgcgctgtt ccatattcga aatgaaaatt tcgcgccatt ttttcggtga
     3541 agctatcaag caaaccgcat cccgccagat accccaagcc aaaaaatagc gcccccagaa
     3601 ttagacctac agaaaaattg ctttgcagta acggttcata aacgtaaatc ataaacggtc
     3661 cggtcaagac caggatgaaa ctcatacacc agatgagcgg tttcttcaga ccgagtttat
     3721 cctgaacgat gccgtagaac atcataaata gaatgctggt aaactggttg accgaataaa
     3781 gtgtacctaa ttccgtccct gtcaacccta gatgtccttt cagccaaata gcgtataacg
     3841 accaccacag cgaccaggaa ataaaaaaga gaaatgagta actggatgca aaacgatagt
     3901 acgcatttct gaatggaata ttcagtgcca taattacctg cctgtcgtta aaaaattcac
     3961 gtcctattta gagataagag cgacttcgcc gtttacttct cactattcca gttcttgtcg
     4021 acatggcagc gctgtcattg cccctttcgc cgttactgca agcgctccgc aacgttgagc
     4081 gagatcgata attcgtcgca tttctctctc atctgtagat aatcccgtag aggacagacc
     4141 tgtgagtaac ccggcaacga acgcatctcc cgcccccgtg ctatcgacac aattcacaga
     4201 cattccagca aaatggtgaa cttgtcctcg ataacagacc accacacctt ctgcaccttt
     4261 agtcaccaac agcatggcga tctcatactc ttttgccagg gcgcatatat cccgatcgtt
     4321 ctgtgttttt ccactgataa gtcgccattc ttcttccgag agcttgacga catccgccag
     4381 ttgtagcgcc tgccgcaaac acaagcggag caaatgctcg tcttgccata gatcttcacg
     4441 aatattagga tcgaagctga caaaacctcc ggcatgccgg atcgccgtca tcgcagtaaa
     4501 tgcgctggta cgcgaaggct cggcagacaa cgcaattgaa cagagatgta accattcgcc
     4561 atgtcgccag cagggcaagt ctgtcgtctc taaaaaaaga tcggcactgg ggcggaccat
     4621 aaacgtaaat gaacgttccc cttgatcgtt cagatcgaca agcaccgtgg atgtccggtg
     4681 ccattcatct tgcttcagat acgtgatatc gactccctca gttagcagcg ttctttgcat
     4741 taacgcacca aaaggatcat cccccacccg acctataaac ccacttgttc cgcctaatct
     4801 ggcgattccc accgcaacgt tagctggcgc gccgccagga caaggcagta ggcgcccgtc
     4861 tgattctggc aagagatcta cgaccgcatc ccctaaaacc catactttgg ctgacatttt
     4921 tttcccttaa attcatctga gttacgcata gtgataaacc tctttttcgc aaaatcgtca
     4981 tggatttact aaaacatgca tattcgatca caaaacgtca tagttaacgt taacatttgt
     5041 gatattcatc gcatttatga aagtaaggga ctttattttt ataaaagtta acgttaacaa
     5101 ttcaccaaat ttgcttaacc aggatgatta aaatgacgca atctcgattg catgcggcgc
     5161 aaaacgccct agcaaaactt catgagcacc ggggtaacac tttctatccc cattttcacc
     5221 tcgcgcctcc tgccgggtgg atgaacgatc caaacggcct gatctggttt aacgatcgtt
     5281 atcacgcgtt ttatcaacat catccgatga gcgaacactg ggggccaatg cactggggac
     5341 atgccaccag cgacgatatg atccactggc agcatgagcc tattgcgcta gcgccaggag
     5401 acgataatga caaagacggg tgtttttcag gtagtgctgt cgatgacaat ggtgtcctct
     5461 cacttatcta caccggacac gtctggctcg atggtgcagg taatgacgat gcaattcgcg
     5521 aagtacaatg tctggctacc agtcgggatg gtattcattt cgagaaacag ggtgtgatcc
     5581 tcactccacc agaaggaatc atgcacttcc gcgatcctaa agtgtggcgt gaagccgaca
     5641 catggtggat ggtagtcggg gcgaaagatc caggcaacac ggggcagatc ctgctttatc
     5701 gcggcagttc attgcgtgaa tggaccttcg atcgcgtact ggcccacgct gatgcgggtg
     5761 aaagctatat gtgggaatgt ccggactttt tcagccttgg cgatcagcat tatctgatgt
     5821 tttccccgca gggaatgaat gccgagggat acagttaccg aaatcgcttt caaagtggcg
     5881 taatacccgg aatgtggtcg ccaggacgac tttttgcaca atccgggcat tttactgaac
     5941 ttgataacgg gcatgacttt tatgcaccac aaagcttttt agcgaaggat ggtcggcgta
     6001 ttgttatcgg ctggatggat atgtgggaat cgccaatgcc ctcaaaacgt gaaggatggg
     6061 caggctgcat gacgctggcg cgcgagctat cagagagcaa tggcaaactt ctacaacgcc
     6121 cggttcacga agctgagtcg ttacgccagc agcatcaatc tgtctctccc cgcacaatca
     6181 gcaataaata tgttttgcag gaaaacgcgc aagcagttga gattcagttg cagtgggcgc
     6241 tgaagaacag tgatgccgaa cattacggat tacagctcgg cactggaatg cggctgtata
     6301 ttgataacca atctgagcga cttgttttgt ggcggtatta cccacacgag aatttagacg
     6361 gctaccgtag tattcccctc ccgcagcgtg acacgctcgc cctaaggata tttatcgata
     6421 catcatccgt ggaagtattt attaacgacg gggaagcggt gatgagtagt cgaatctatc
     6481 cgcagccaga agaacgggaa ctgtcgcttt atgcctccca cggagtggct gtgctgcaac
     6541 atggagcact ctggctactg ggttaacata atatcaggtg gaacaacgga tcaacagcgg
     6601 gcaagggatc cgcgtcactc ttcccccttc acgaccttca ataatatgca atgcagcttc
     6661 ccgcccgata atgtcatgtg gaagctgaat tgtggtcagc ggcggtaaaa acagatgccc
     6721 gacgccaacc agattatcaa agcccattac ggcgacatcc tgcgggatac gtaccccctt
     6781 cgccaggaga acctgataag cgacaaaggc tgcgcgatcg ttaccacata tcagaacatc
     6841 aaaatctggt ttgcccggtt tgaagtgggc attgagtaaa cttgcgagat cggtgtagtg
     6901 atcatcacct gttgccatgt gaaattgttt cacctcagcc agatctcgtc cagcatcacg
     6961 ccaggcctgc tcaaatccct gccgacgata ccctgttgcc aacgcacttt ccggtagcca
     7021 gaagcataac ggttgacgat agcccgccgc gagcaaatgc tgtgttgatt catattgtgc
     7081 agtgtaatca tcagggatat aactgggtaa cgctgggtca tccgccacac agttcgccaa
     7141 tacaatattt tcaccataca gagactcagg cagcgtgata tgtcgcagcc ccattgtagt
     7201 atagataatg ccatccggac ggtgggcaag cagctgacgt gccgcgcggg cagcgtcatc
     7261 ttcagaaaaa atattgatta aaaaactatt ccagccgaac tcgctggcgg tttgctcaat
     7321 ggcaagcaga atatcaacag agaaaggagt ggtagccgtg tcctgcgcca gcacggcgag
     7381 agtcgacggc ttacgtcctt gagcgcgcat cttacgggcg gaaagatcag gaacataatt
     7441 cagggtctgg attgcctgca atacgcggtc acgcgttgca ggacgcacag attctgcatt
     7501 atgcatcacc cgggagactg tcatcatcga cactcccgcc aggcgtgcga catcctttaa
     7561 tgaagccata cccaagccgt ttgccgtaaa acgggcactg tagcagaaac agacgtcact
     7621 ggcgagatcc aacgatattc tcaacgccct atcacccaac acggcaatac aataaaaaat
     7681 aacaataatt gccggataat tgtactcaat tccgcctctg ttctcgccaa cgagtaacca
     7741 tataggcaag taagtaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa
     7801 tatggatcat tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta
     7861 tcggttcagg tgcaattggc tgcacgatcg ttacggactc gcttttctgg ctagtgaagc
     7921 aatattgcgg cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg
     7981 cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag
     8041 acgtactatg gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga
     8101 tctggttgct cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg
     8161 tttaccttat gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg
     8221 ttttgcaccc tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga
     8281 atcagaactg gttgccattc cagctatgca aaaacggctg gaaaaagaat atcagcaacc
     8341 gatcagcggg caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa
     8401 agcacgcggc gggatttatg aagtcctggc acacgcagaa aaactggctc tggaagcggg
     8461 gttgctgacg cttgatgatg actacagcaa actgctttct ccggagttta aacagttctt
     8521 tagccaatac agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat
     8581 gagcgcccgc attggcttta aggtgacagt tcatatgtct gctgatgccc gggcatggaa
     8641 aaaagcgaaa ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt
     8701 tgccgtcgag gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga
     8761 cgaaaattcc cgcacgttgt tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca
     8821 atttgcccag caaggccgta tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg
     8881 tggtgttggc ggtggtcctg gtggcgtcgc attcgggctt aaactggcgt ttggcgatca
     8941 tgttcactgc ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac
     9001 aggattacac gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga
     9061 tggccttgca gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga
     9121 tggcttctat acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga
     9181 agaaggtatt cgtcttgaac cttcagcact ggcgggtatg gccggacctc agcgcgtgtg
     9241 tgcatcagta agttaccaac agatgcacgg tttcagcgca gaacaattgc gtaatgccac
     9301 tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa gaagagatga atcaatatct
     9361 ggcaaaaggc cgttaataac gtttcaacgc agcatcgcaa tcctttccct gggtgagcga
     9421 tgctgccgat ggcgcagact taagatcccc ggtcttaccc gctataaccc cctcctttta
     9481 tcatcaccca acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag
     9541 taagataaaa gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg
     9601 ctgggttatt tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc
     9661 cataatttgt gacgatgaat taaacacggg gttaaattga tcgatggttg ctgtcaactg
     9721 actatggtgt aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga
     9781 tcctgacaag gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg
     9841 caagcctgaa aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg
     9901 aaaaaactgc ggcaaaatga tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg
     9961 ccagtaatag caaaccgcat acatcaaaaa actaaatgtc actaacaacc gcatgtctat
    10021 tttgttgcca taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc
    10081 ataagcaagt ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat
    10141 aaggacgatc gctccagagt aaaataaata cgcgcatgtg atactcacaa taccaatggt
    10201 gaagttacgg gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca
    10261 aatgactaaa gagatcagag aaataactga tactactgtt aatattatta ttgtactcga
    10321 gttgaaccaa tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag
    10381 cactaacagg gtcagtcctg gtagattcat tttgaccggt gaagtttcag tttctcttcc
    10441 tttaagtaag gttaagcata atgtcaggac gataatcccc atagggacat tgattaaaaa
    10501 tatccaaccc cagctaaagt tatcgcaaat ataaccgccc aatatcggcc cacatatcgg
    10561 agcgataatc acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata
    10621 attccttaat aataaactct gtgacagtgg aattaacggc cccgccatta atccctgaac
    10681 gactctaaaa aatatcagca catcaagatt ggtcgataaa ctacacatta aggaagacag
    10741 cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct
    10801 gccggtaaca gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc
    10861 ttcgtctgtt gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt
    10921 ggaatccaac atttgcataa acgtcgctaa tgacaatgca atagtaacgc accataacgt
    10981 cccaccggtt aatggtgccg gagtcgattt agtgattgcc attaaagttg tccattatgc
    11041 gaaataatgt tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg
    11101 gtataagccg gcatggacgt cacggttgaa gccagatcag gcatctcagc aatgtcttcg
    11161 ttcttcgtat caatagttgc agtcatcgat aaaccaatgc gtaaggggtg ttccatgagt
    11221 tcttttggat caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct
    11281 gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc
    11341 acccgaccat gaaacacaac attttcacca taaagatcgc tgataatatt gactgattga
    11401 ccaatccgta catccgtgag ttgtgtttct ttaaagttgg cattaaccca catttgacgt
    11461 gccggtacga cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactc
    11521 cgctgggcaa tatagccagt aaccggactc cttatatccg tacgtttaag cgccaaccag
    11581 gtttctttag ttgcatccgc cgcttccacg acttgtggct gacggtttaa tggtgtgttc
    11641 attactaaag ctttattcgc tttataagcc tggatagcgg cattcagtgc cgctttgcta
    11701 cttattaacg tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct
    11761 aacggtactc gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg
    11821 acttcggcac tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt
    11881 gccagattat ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact
    11941 aaaatgtcac cttgtcgaac gtagttcgta tctttatgat taacgacagt gacactacct
    12001 gagacttgtg cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc
    12061 atgtcttcta attccattga ccaataggca taggcacctg aaaacgcaat aaataaaact
    12121 accgccaata aagaaaagta ttttcttctg ttagaa
//
LOCUS       DAZGCS010000130        12033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGCS010000130 DAZGCS010000000
VERSION     DAZGCS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.132"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            177..740
                     /locus_tag="ACHK01_004186"
     CDS             177..740
                     /locus_tag="ACHK01_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099037.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            822..3464
                     /gene="yfcU"
                     /locus_tag="ACHK01_004187"
     CDS             822..3464
                     /gene="yfcU"
                     /locus_tag="ACHK01_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO8099038.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3484..4236
                     /locus_tag="ACHK01_004188"
     CDS             3484..4236
                     /locus_tag="ACHK01_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8099039.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4211..4759
                     /locus_tag="ACHK01_004189"
     CDS             4211..4759
                     /locus_tag="ACHK01_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099040.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4756..5226
                     /locus_tag="ACHK01_004190"
     CDS             4756..5226
                     /locus_tag="ACHK01_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099041.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5223..5747
                     /locus_tag="ACHK01_004191"
     CDS             5223..5747
                     /locus_tag="ACHK01_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099042.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5734..6606
                     /locus_tag="ACHK01_004192"
     CDS             5734..6606
                     /locus_tag="ACHK01_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO8099043.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTIYNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6728..7279)
                     /gene="smrB"
                     /locus_tag="ACHK01_004193"
     CDS             complement(6728..7279)
                     /gene="smrB"
                     /locus_tag="ACHK01_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO8099044.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7445..8377
                     /gene="prmB"
                     /locus_tag="ACHK01_004194"
     CDS             7445..8377
                     /gene="prmB"
                     /locus_tag="ACHK01_004194"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8099045.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8412..9497
                     /gene="aroC"
                     /locus_tag="ACHK01_004195"
     CDS             8412..9497
                     /gene="aroC"
                     /locus_tag="ACHK01_004195"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO8099046.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9501..10325
                     /gene="mepA"
                     /locus_tag="ACHK01_004196"
     CDS             9501..10325
                     /gene="mepA"
                     /locus_tag="ACHK01_004196"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO8099047.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10325..11134
                     /locus_tag="ACHK01_004197"
     CDS             10325..11134
                     /locus_tag="ACHK01_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO8099048.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11134..11682
                     /gene="epmC"
                     /locus_tag="ACHK01_004198"
     CDS             11134..11682
                     /gene="epmC"
                     /locus_tag="ACHK01_004198"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO8099049.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11716..11994
                     /locus_tag="ACHK01_004199"
     CDS             11716..11994
                     /locus_tag="ACHK01_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO8099050.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2987 a         2918 c         3133 g         2995 t
ORIGIN      
        1 atcagggggg gggaaacatc actatgcggc cccacaccaa agaatacaat tttctacgac
       61 ctgttctttt tggatcgcat gccgtattca atggctgggt ataaacattg ggtattgact
      121 gaatcaatgt attgtaatgt ttatattttt aattatttat agtttaagga caaataatga
      181 gtaagtttgt taaaacagct attgctgcgg caatggtgat gggcgttttc acctctacgg
      241 caacaatcgc tgcgggtaac aacggtactg cacgttttta cggcaccatt gaagactcag
      301 tgtgttctat cgttccggac gatcacaaac tggaagttga tatgggtgat atcggtgccg
      361 aaaaactgaa aaataacggc accaccacgc cgaaaagttt ccagattcgt ctgcaagatt
      421 gcgtatttga tactcaggaa acaatgacca ctacctttac tggtaccgtt tcttctgcaa
      481 atagcggcaa ttattacacc attttcaata ccgataccgg tgcggcattt aacaatgtta
      541 gcctggcgat cggtgactct ctgggtacct cttacaaaag cggcatgggt attgaccaga
      601 aaatagtgaa agacacttct accaataaag gcaaagcgaa gcagacactg aactttaaag
      661 cctggctggt cggcgcagct gatgcgccag atctgggtaa tttcgaagca aataccacct
      721 tccagattac ttacctgtaa ggcatacccc catagcgaca ctgctatggg ggatttaaat
      781 ggattttgac cgaacaagaa taaaaacgga tagaaacgtg tatgcctgac cattctcttt
      841 ttcgattacg gattcttccg tggtgcattg cgctggcaat gtcagggagt tatagcagtg
      901 tctgggctga agacgacatt cagtttgatt cccgttttct ggaattaaaa ggcgacacaa
      961 aaattgatct gaagcgtttt tccagccagg gatatgttga gcccggaaaa tacaatttac
     1021 aggttcaact aaataaacag ccattggcgg aagagtacga tatttactgg tatgctggtg
     1081 aagatgacgc gagcaaaagc tatgcttgtc tgacaccgga actggtagcg cagtttggtt
     1141 taaaagaaga cgtggcgaac aatctgcaat ggagccacga tgctaaatgc ctgaaatccg
     1201 gtcaactgga aggcatggaa attaaggctg atttaagcca gtccgcatta gtcatttcac
     1261 tgccacaggc ttacctcgaa tatacttatc ccgactggga tccgccttca cgttgggatg
     1321 acggcatctc cgggatcgtc gcggactaca gcatcaacgc acaaacccgg cacgaagaaa
     1381 atggcggtga tgatagtaac gagatcagcg gcaacgggac ggtcggggtt aacctggggc
     1441 cgtggcgtat gcgtgctgac tggcagacta actatcaaca tactcgcagt aatgatgacg
     1501 atgaattcag cggcgatgaa actcaaaaaa aatgggagtg gagtcgctac tatgcctggc
     1561 gggcgttacc atcattaaaa gccaaactgg cgctgggcga ggattacctc agatccgata
     1621 tttttgatgg ttttaactat gttggtggca gtgtcagtac tgacgatcaa atgttgcctc
     1681 ccaatctgcg cggctacgcg ccagacattt ccggcgtggc acacaccaca gcaaaagtga
     1741 ccgtcagcca gatggggcgt gtgatttacg aaacgcaggt tccggctgga ccgtttcgta
     1801 ttcaggatct tggtgattct gtctccggta cgttgcatat tcgcattgaa gaacagaacg
     1861 gccaggtgca ggaatatgac atcagcaccg cctcgatgcc atacctcact cgcccaggtc
     1921 aggttcgcta taagatcatg atgggccgtc cgcaagagtg gggacaccat gtcgagggtg
     1981 aatttttttc tggtgctgaa gcttcctggg ggatcgctaa cggctggtcg ttatatggcg
     2041 gcgcactggg agatgaaaac tatcagtctg cggcgcttgg cgtcggtcgc gatttgtcta
     2101 cattcggcgc ggtcgcgttt gatgttactc actcgcacac caaactggat aaagacaccg
     2161 cttatggcaa aggttcgctg gacggtaact ccttccgtgt gagttattcc aaagactttg
     2221 accagctcaa cagccgcgtt acttttgctg gatatcgctt ctcggaagag aactttatga
     2281 ccatgagcga gtatctggat gccagtgaca gcggaatggt acgcacgggc aacgacaaag
     2341 agatgtacac cgccacttat aaccagaact tccgcgatgc gggtgtttcg gtttatctca
     2401 actatacccg ccatacctac tgggatcgcg aggagcagac aaactacaac atcatgctct
     2461 cgcactattt caatatgggc agtattcgta atgtcagcat ctcgatgact ggctaccgtt
     2521 acgagtatga caaccaggcc gacaaaggca tgtacatttc gctcagtatg ccgtggggcg
     2581 acaacagtac cgttagctat aacggtaact atggcagtgg gacggacagc agtcaggtcg
     2641 gttatttcag ccgtgtcgat gacgcgactc actatcagtt gaacgtcggc accagtgaca
     2701 aacacaccag cgttgacggc tattacagcc atgatggttc gctggcgcag gttgacctca
     2761 gtgcgaacta ccatgaaggg caatacacct ctgcgggctt gtcgttacag ggcggcgcaa
     2821 cgcttactgc ccacggtggc gcacttcacc gtacccagaa tatgggcggg acacgcttgt
     2881 tgattgatgc cgatggcgtt gccgatgttc cggtagaagg taacggtgct gctgtttata
     2941 ccaatatgtt tggtaaagcc gtcgtttctg acgtcaataa ctattaccgc aatcaggcgt
     3001 atatcgacct caacaaactg ccggaaaacg ccgaagcaac ccagtcggtg gtacaagcca
     3061 cgctaactga aggggcgatt ggctaccgca aatttgccgt catcagtggt caaaaagcga
     3121 tggcggtgct gcgtttacaa gacggcagcc atcctccgtt tggcgcagaa gtaaaaaatg
     3181 ataacgagca gacagtgggc cttgtcgatg atgacggcaa tgtttatctg gctggggtga
     3241 aacctggcga acacatgagt gtgttctgga gtggtgttgc gcattgcgat atcaacctgc
     3301 cggacccgct gcctgccgat ctgtttaacg gcttgttact gccatgccag cataaaggca
     3361 acgtcgcgcc ggttgttcct gatgacataa agcctgtcat tcaggagcag acgcaacagg
     3421 tgacacccac caatccccct gtttctgttt cagctaacca ataacgtgat taaggaatga
     3481 tccatgtctg atttactttg ctctgccaaa ttgggtgcta cgacgctagc gttactgctt
     3541 agcgctgcca gtttgtctgc ccaggcttct gtaacgccag atcgtacccg tttgatattt
     3601 aatgaaagcg acaaatcaat cagcgtgacg ttgcgcaata acgatccgaa actgccttat
     3661 ctggcccaaa gctggatcga agatgaaaag ggtaacaaga tcagttcacc gttaactgtt
     3721 ctgccaccgg tgcaacgcat cgattcaatg atgaacgggc aggtaaaagt gcagggaatg
     3781 ccggatatca acaaattgcc tgccgatcgt gaaagcctgt tctacttcaa cgtgcgtgag
     3841 atcccgccaa aatcaaacaa agccaacacg ctgcaaatag cactacaaac gcgtattaag
     3901 ttgttctggc gaccgaaggc gctggagaac gtcagcatga aaaacccctg gcaatataag
     3961 gttactttaa cccgcaacgg tcaggaattt accgtcaata acccgacacc gtattacgtc
     4021 attttcagta atgccagcac acagaaaaat ggcaatccgg cagccggttt ctcgccaata
     4081 gtgatgtcgc cgaaaacctc tgcaccgctg aatgtgaaga tgggtagcgt gccggtactg
     4141 acctatgtca acgactatgg cgctcggatg ccgctctttt tcacttgcaa cggtaacagt
     4201 tgccaggtcg atgaagagca gagccgcaaa ggctgaggcc gggagagcca gtatgaaagg
     4261 atcttgttct gtctgggcgg taactgggtt actgatatgt tgtgttggta gcggctttgc
     4321 agttgcagcg agtaagtcag taagtatgaa tttacgggtg cttgtcgatg cccctcctcc
     4381 ttgtacggta aatggtgcgg cagttgagtt tggtaatgta ttcattaata agatcaatgg
     4441 ggttgattat aaacgtccca ttgactactc gcttgtttgt aacaatctgg caatggatga
     4501 cctgcgtcta cagatgcagg cgacaacggt cgtgattaac ggtgaaacag ttatcagcac
     4561 ggggattcct gggtttggta ttcgggttca gaaaagtagt gatcacacaa tactcgactt
     4621 aacctctggc tcatggttgc catttaattt ttcctcggga gttcccgttc tggaagctgt
     4681 gccggtgaaa cagagtggta caacgttagc ggcagctgaa tttaacgcca gtgccacaat
     4741 tgtggtggat taccaatgaa acggatctcg cttattttgc tgtggggctt ttgctcgatg
     4801 gcactctcaa acgtctcttt tcacggttat ttagtgcagc cacctaactg cacgattagc
     4861 aacgctcaaa ccattgaaat aacttttcag gatgtgctta ttgatgacat taatggcagc
     4921 aattatgagc aaaccgtgcc gtattccatt acctgcgata cggcggttcg cgacccatta
     4981 atggaaatga ccttgagctg gagtggcaca ccaagcgatt ttgataatgc tgcagtaagc
     5041 agcaatatca ctggtttagg cattcagctc aaacaggcag ggcagagttt tacgatcaat
     5101 acgccacttg tggtgaatga aaccgattta ccggtgttaa cggctgtgcc agtaaaaaag
     5161 agtggtgtcg ttctgccaga ggcggacttt gaggcctggg ccacgttaca ggtagattac
     5221 caatgaagaa aaagcgtacg ttgtttttta ttagtagcct gatgttgctg ggcagtggaa
     5281 ccacaatagc aggagataat ttgcacttta ccggcaattt aatcagtaaa tcgtgtacgc
     5341 cagtgattaa tggtagccag ttggcagaag tacattttcc tgccattgcc gccagcgatt
     5401 taatgaacct tgggcagtca gagcgagtgc cattggtgtt tcaattgaaa gattgtcata
     5461 gttctacatt gtttaatgtc aaagtaacgt tgacaggaac agaagatagt gctctacccg
     5521 gctttctggc ctttgactcc tcgtcatcag catccggcgc tgggattggt attgaaactg
     5581 cggcaggcac atcggtgcca atcaacaata caacaggcgt cacgctcccg ctgaatcagg
     5641 gaaataacag cctaaatttt aatacctggt tacaggcaaa gagcgggcga gatgtaacgt
     5701 ccggagactt tagtgccaca gtgacggcaa cctttgagta tttctgaaat gaaaaaatgg
     5761 accataattt tcgcgagcct aatgctgctc gttctgagtg ttgtcggcgc atctaagagt
     5821 tatgctggag ataaacttat gagtgcgagt tttgacagtg tgagagttta ttatgccatg
     5881 gacaaggtaa ccggtgctgt agcgagcagt gttttcaatg ttacggtgat aacgccaaag
     5941 gaggttgcat atgggaaata tgattctttt gcgtataaag gcgatacgtt gcgggttatt
     6001 tcctggtctg ggtcgggttc agctccaact ttggtactga ctgactttga tacaatttat
     6061 aactcaaatt gccctggcat agatacgaaa atttttcgtt gtgcatatat gacgtttaaa
     6121 attaccgttg catctgatga ttatggttgc ccttggatcg catcctttta ttcctataca
     6181 gatttacccg gatttggaag ctatacggcc cctacagtgc ataacacaat atgcccaacg
     6241 ataccggtcg cttcatacga tatctcctgg agtgaaaatt atgtcagcca taataaggcg
     6301 ttacgaattc agtccacggg gtcaacagtg accaccacgc tttcgaccta cttgatggaa
     6361 ggcggcagac tctgtgatgg cagtaatttt tctgataatg atggcagagg agcatactgc
     6421 cgcgcggtat cagaattgtt aacctttacc tcttacggtt gtgataaatc aacggtaacg
     6481 gttacgccaa ctcgtcatcc ggtaacggat aaagtactac acgatattgt ggtgaatgtg
     6541 aataccagta gtgggcaacc gattgattcg acgtgtcgtt ttcagtatgt attgaatgaa
     6601 ctgtagttcg tagtattgct ctgatgcgtc ttatcaggcc tacgaagtga attgcaattt
     6661 attgaattat cacatatttg taggccggat aaggcgttta cgctgctccg gcatttgctg
     6721 ccagcattca gggcaactcc ggcggcaacc actcttccac ttcaatcaac accaacaaag
     6781 ccgcatcacc gccgtactct ttcggtgcct ggtgaaatgc catcacatgc ggatgttgtg
     6841 ccagccacag cggtgtttgt tgcttcaaaa tatgcttccc gtgcccatgc atcacgcagg
     6901 cacaaaacac atgttcgcgg cgacaggcgg caatcaacgc gcccagttcc tgcttggcct
     6961 gcagctgcgt cagaccgtgt aaatccaaaa acaattccgg tgaataatcg ccgcggcgca
     7021 gtttcttcgc ctcaaaatgg ctgacatccg ggcgaacata tttcaccgga ccttcggtat
     7081 ttaataacgg ctgaaactca tcggagaaat aatggctggc atcagcctgc tcctggatca
     7141 agcgtttcac cggcacttcg ctgatttttt tacgctgcgg tcggtggaca atcgtgtcct
     7201 gcttaatctt gcgagtcccc gccatcaact ggcgaaacag agcctggtcc tcctcgctaa
     7261 gtgttgtttt ctttttcatt tatctgtctc tatttctaat tttcctacag tttacccgac
     7321 tcgtagcaca atcgatcgtt ttaaacgtat ttttcctcac tcaccgcgcg tgaatgctga
     7381 tttatcgtca tcttcatggc aaactagccg ccgaaattat tgcgatcatg ccctggagga
     7441 atacgtggat aaaattttcg ttgatgaagc agtaaatgag ctgcaaacca ttcaggacat
     7501 gttgcgctgg tcggtaagcc gcttcagcgc ggcaaatatc tggtacggtc acggcaccga
     7561 taacccgtgg gatgaagccg tacagctggt gttaccatcg ctctacctgc cgctggatat
     7621 cccggaagat atgcgcaccg cgcgtctgac ctccagcgaa aaacaccgta ttgttgaacg
     7681 cgtgatccgc cgcgtcaacg agcgcattcc ggtggcttac ctgaccaaca aagcctggtt
     7741 ctgcggtcat gaattttacg tcgatgaacg cgtgctggtg ccgcgctcac cgattggtga
     7801 actgatcaac aataaatttg ccggactcat cagcaagcaa ccgcagcata ttttagatat
     7861 gtgtaccggt agcggctgca tcgccattgc ctgtgcttat gccttcccgg aagcggaagt
     7921 cgacgcggta gatatctctc ccgacgcgct ggcggttgcc gaacagaaca tcgaagaaca
     7981 cggtctgatc cacaacgtca ttccgattcg ttccgatctg ttccgtgatt tgccgaaagt
     8041 gcaatacgat ctgattgtca ctaacccgcc gtatgtcgac gcggaagata tgtccgacct
     8101 gccaaacgaa taccgtcacg agccggaact gggcctggca tctggcaccg acggcctgaa
     8161 actgacgcgt cgcattctcg gtaacgcggc agattacctt gcggatgatg gcgtgttgat
     8221 ttgtgaagtg ggcaacagca tggtacatct tatggaacaa tatcccgatg ttccgttcac
     8281 ctggctggag tttgataacg gcggcgatgg agtgtttatg ctcaccaaag agcagcttat
     8341 tgccgcacga gaacatttcg cgatttataa agattaagta aacacgcaaa cacaacaata
     8401 acggagccgt gatggctgga aacacaattg gacaactctt tcgcgtaacc accttcggcg
     8461 aatcgcacgg gctggcgctc ggctgcatcg tcgatggtgt tccgccaggc attccgctga
     8521 cggaagcgga cctgcaacat gaccttgatc gtcgtcgccc tgggacatcg cgctatacca
     8581 cccagcgccg cgagccggat caggtcaaaa tactctccgg tgtttttgaa ggcgttacta
     8641 ctggcaccag cattggcttg ttgatcgaaa ataccgacca gcgttctcag gattacagcg
     8701 caattaaaga cgtcttccgt ccaggccatg ctgattacac ctacgaacaa aaatacggtc
     8761 tgcgcgatta tcgcggcggt ggacgttctt ccgcccgcga aaccgccatg cgcgtggcgg
     8821 caggggcgat tgccaaaaaa tatctcgccg agaaatttgg tattgaaatc cgcggctgcc
     8881 tgacccagat gggcgacatt ccgctggaaa tcaaagactg gtcgctggtt gaacaaaatc
     8941 cgttcttctg cccggacccc gacaaaatcg acgcgttaga tgaactgatg cgcgcgctga
     9001 aaaaagaggg cgactccatc ggcgcgaaag tcaccgttgt tgccagtggt gtccccgccg
     9061 gacttggcga gccggtcttt gaccgcctgg atgccgacat cgcccatgcg ctgatgagca
     9121 tcaacgcggt gaaaggcgtg gaaattggcg acggttttga cgtagtggcg ctgcgcggca
     9181 gccagaaccg cgacgaaatc accaaagacg gtttccagag caaccatgcg ggcggcattc
     9241 tcggcggtat cagcagcggg cagcaaatca ttgcccatat ggcgctgaaa ccgacctcca
     9301 gcattaccgt gccggggcgc actattaacc gctttggcga agaagttgag atgatcacca
     9361 aaggccgtca cgatccctgt gtcgggatcc gtgcagtgcc gatcgcagaa gcgatgctgg
     9421 cgatcgtttt aatggatcac ctgttacggc agcgggcgca aaatgccgat gtgaagactg
     9481 atattccacg ctggtaaaaa atgaacaaaa ccgcgattgc gctgctggct ctgcttgcca
     9541 gtagcgctag cctggcagcg acgccgtggc aaaaaataac ccaacctgtg ccgggtagcg
     9601 cacaatcgat aggcagtttt tctaatggct gtattgtcgg cgctgacacg ttgccgatac
     9661 agtccgaaca ttatcaggtc atgcgtaccg atcagcgtcg ctatttcggt cacccggatc
     9721 tggtgatgtt tatccagcgt ctgagtagcc aggtgagcaa tctgggcatg ggtacggtgc
     9781 tgattggcga tatggggatg cccgctggtg ggcgtttcaa cggcggtcat gccagccacc
     9841 agaccggact ggatgtcgat atctttctgc aactgccgaa aactcgctgg acctccgcgc
     9901 agctcttgcg cccgcaagca ctggacttag tttcccgcga cggtaaacac gttgttccga
     9961 cgctgtggaa gccagaaatt ttcagcttga tcaaactcgc cgcccaggac aaagacgtca
    10021 cacgcatttt tgttaatccg gcgattaaac aacaactttg ccttgatgcg ggcaccgatc
    10081 gcgactggtt gcgcaaagtg cgaccctggt tccagcatcg cgcgcatatg catgtacgat
    10141 tacgttgtcc tgccgatagt ctggagtgtg aagatcaacc tttaccgcca ccaggcgatg
    10201 gttgcggggc agaactgcaa agctggtttg cacctccaaa accgggaaca acaaagcctg
    10261 agaagaagac accgcctccg ttgccgcctt cctgccaggc gctactggat gagcacgtga
    10321 tctaatggaa acgtttaata gcctgtttat ggtttccccg ctgttgctgg gagttctctt
    10381 ttttgtcgcc atgctggcgg gatttatcga ctcgattgcc ggtggcggtg ggttactcac
    10441 cattccggca ttgatggcag cgggaatgtc tcccgctaac gcgttggcaa ccaataaact
    10501 gcaagcctgc ggcggctcta tttccgccac tatctacttt attcgccgca aagtggttag
    10561 tttaagcgat cagaaactca atattgccat gacctttgtc ggctcaatga gcggcgcact
    10621 gttggtacag tacgttcagg ctgatgtctt acggcagatt ttgcccattc tggtgatttg
    10681 tatcggcctc tatttcttgc tgatgccaaa gctgggtgaa gaagatcgcc agcgccggat
    10741 gtacggttta cccttcgcgt taattgccgg tggctgcgtc ggtttttatg acggattctt
    10801 tggcccggct gccggatcgt tttacgcgct ggccttcgtt acgctgtgcg gattcaacct
    10861 cgccaaagcc acggctcacg ccaaattact caacgcaacg tcaaacattg gcggtctgct
    10921 gctgtttatt ctcggcggca aagtgatttg ggcgacgggc tttgtgatgc tggtggggca
    10981 gttcctcggc gcgcgcatgg gttcacggct ggtgttgagc aaaggacaaa agctgatccg
    11041 cccgatgatt gttatcgtct cggcggtgat gagtgccaaa ttactttacg atagccacgg
    11101 acaggagatc ctccactggt tggggatgaa ctaatgaaca gtacacacca ctatgagcag
    11161 ttgattgaaa tttttaatag ctgctttgcc gatgaattta atacccgtct gattaaaggc
    11221 gacgacgaac cgatctatct tcctgctgat gcggaagtac cgtataaccg aattgtcttt
    11281 gcccacggct tttatgccag cgcaattcat gagatttcgc actggtgtat tgccggaaaa
    11341 gcgcgccgtg agctggttga tttcggctac tggtattgcc cggatgggcg tgatgcccaa
    11401 acgcaaagcc agtttgaaga tgttgaagtg aagccgcagg cactcgactg gttgttctgt
    11461 gtggcggcgg gatatccgtt taacgtcagt tgcgacaatc tggaaggcga cttcgaaccg
    11521 gatcgtgtgg tgtttcaacg gcgggtacat gcgcaggtga tggattacct agcaaacggc
    11581 attcctgaac gcccggcacg cttcattaaa gcactacaaa attattatca cacgccggaa
    11641 cttacggcgg aacagttccc gtggccggaa gcgctcaact aaagccagcg ccacgtttta
    11701 caatgaggaa agaagatgat cgcggagttt gaatcacgca ttctggcatt aatcgacggt
    11761 atggttgacc atgccagtga tgatgagttg tttgccagtg ggtatttgcg tggccacctg
    11821 acattagcca tcgcagaact ggaaagtggt gatgaccact ccgctcaggc ggtgcatacg
    11881 accgttagcc agagtctgga aaaagccatt ggtgccggtg aattgtcgcc gcgtgaccag
    11941 gcgctggtga ccgatatgtg ggaaaacctg tttcagcagg cgtcacagca gtaagttgcg
    12001 gcgtctggag caccatgcct gatgcgacgc tta
//
LOCUS       DAZGCS010000131        11938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGCS010000131 DAZGCS010000000
VERSION     DAZGCS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.133"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6012
                     /gene="efa1"
                     /locus_tag="ACHK01_004200"
     CDS             <1..6012
                     /gene="efa1"
                     /locus_tag="ACHK01_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO8099051.1"
                     /translation="PLAYDYILNKKESSSIPVEILNKLSELPPHELLTPVLGQSVNPL
                     GMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGTLNSQNLRQL
                     LENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPGT
                     GKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVASSLHFIAEHAKYT
                     LLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTKDTAHY
                     SILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLHK
                     TAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDALK
                     FIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGLSTRIQHTTT
                     DRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLNGDVLNDYLS
                     RTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQQL
                     AGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHTTSVEVPEVV
                     SRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKINLAMDIKQL
                     SEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKALSASARVLE
                     LPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAASVAFPPLIIA
                     TGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPERGLLDFSGNK
                     VFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSISPYSSLAHG
                     YANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVPDSTSRHKRP
                     PLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRGGSGGLAVQV
                     GGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLKTPDDEVPVMTITQK
                     GINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTLSS
                     NSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKLNHFAGNFRVHTRDG
                     MTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHDMGWSLTPEVRFQGG
                     ETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRYIIMKPVTTQILPTQ
                     IILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISGIEESLPETT
                     RVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQVMTFLPRVSG
                     SAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYLIF
                     EGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSLILSEFRRFT
                     ISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTTDR
                     EILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQKEETSKILY
                     QYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDLSGRYKSVPMARKAD
                     TLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVVHS
                     ILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGRVYSREEYMM
                     WELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVSKWSRGWLKT
                     GTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNAPGETRCTLR
                     PGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6184..6534
                     /locus_tag="ACHK01_004201"
                     /pseudo
     CDS             6184..6534
                     /locus_tag="ACHK01_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6666..7091
                     /locus_tag="ACHK01_004202"
     CDS             6666..7091
                     /locus_tag="ACHK01_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8099052.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGHVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTMLIRELSGRGR"
     gene            7088..7438
                     /gene="tnpB"
                     /locus_tag="ACHK01_004203"
     CDS             7088..7438
                     /gene="tnpB"
                     /locus_tag="ACHK01_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8099053.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            7469..9054
                     /locus_tag="ACHK01_004204"
                     /pseudo
     CDS             7469..9054
                     /locus_tag="ACHK01_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            9083..9226
                     /locus_tag="ACHK01_004205"
                     /pseudo
     CDS             9083..9226
                     /locus_tag="ACHK01_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9475..9630)
                     /locus_tag="ACHK01_004206"
                     /pseudo
     CDS             complement(9475..9630)
                     /locus_tag="ACHK01_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(9917..10342)
                     /locus_tag="ACHK01_004207"
     CDS             complement(9917..10342)
                     /locus_tag="ACHK01_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8099054.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(10339..10722)
                     /locus_tag="ACHK01_004208"
     CDS             complement(10339..10722)
                     /locus_tag="ACHK01_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8099055.1"
                     /translation="MYAKSFLALDGNGRLTGARTAHTAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERKEIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            complement(10890..11459)
                     /locus_tag="ACHK01_004209"
     CDS             complement(10890..11459)
                     /locus_tag="ACHK01_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8099056.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            11659..11853
                     /locus_tag="ACHK01_004210"
     CDS             11659..11853
                     /locus_tag="ACHK01_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099057.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
BASE COUNT         3292 a         2877 c         2940 g         2829 t
ORIGIN      
        1 ccactagcat atgattacat tttaaataaa aaagaatcaa gctctattcc tgttgaaatt
       61 ttgaacaaac tttcagagtt accaccacat gaactactca cacctgttct tggccagagt
      121 gttaatcctc tgggcatggg ctactcatcc gataatggaa aaatcacaga gcaagtaata
      181 gtcagtggag ctgatggatt tgataatccc atatctggac ttatatatac ctatcttgaa
      241 gatctatata acatccatgt aaggatgcga gaaggtacac taaattcaca gaatcttcgt
      301 cagcttctgg aaaactctgt ttcttcatgc tttttgactg agcaaagtat taataaatta
      361 cttagtgagg cagaaaaaag gccttatcag tctttaacag aaatacatca gcatctcaca
      421 ggattaccaa ctattgccga tgcaaccctt tcattacttt ctgttggatt acctggtacg
      481 ggaaaactat tgcgcaggga gcaggactat gggcgtccac cagttacagc aattcaggat
      541 tccacatttg tactccctta taatttcaaa ggtattggtt ttaacgataa cattatatcc
      601 tctgcacctg tagcctcctc attacatttt atcgctgaac atgcgaaata tactttattg
      661 tcatggcctg agttttatcg tcatcatgca cagcgatggt tcgaaatggc taaaggatat
      721 ggaagccaga atattgattt tcaccctcag tctctattgg taacccaaga aggacgctgt
      781 atgggattag ccttacttta tttacagact aaagatactg ctcattatag cattctccag
      841 gaaaacctaa tgactgtgag tgcacttcat cagaccagta atcgcgataa gttgccactg
      901 tccaaagatg ataattcctt aatgacaaga acttatagtc tgattgaaat gctacagtat
      961 cagggaaaca aatatattac caacgaatcg ctactacata agaccgcatg gaaccaagaa
     1021 agaataactt tattattcaa tgaaaaagga gttaagcgag ccctgataag cacgcctaat
     1081 catactctgg ttctgcaaca actggaggat atttaccggc tcactgatcc aaattttggg
     1141 catgcagatt tcctttcacc tatagatgct ctgaagttta ttgaggctat gatacaatta
     1201 actccaacac ttcaggaata ttatggccta ttaaacaaag acattaataa acatatacaa
     1261 gtacattatg ccgaatcaga tatggtctgg aataagcttc tgccagaaaa tgatgctgga
     1321 ctgagcacca gaattcagca caccaccacc gaccgtctgg cgaatctggc tgaaccagtc
     1381 gctgttgcag gtatctccct gccagtaaaa acactttatg atatcggagc cacccttgac
     1441 ggtcggcgca tcacctctcc tccaacatcg gagcaaatcc cttctctgcg tctcaacggt
     1501 gatgttctga atgattatct gtcccgcaca gttctgactc cagaacaggc tgataacata
     1561 agaaaaatac tgcacactca gggaatacgc agcggtaccc gtcccataga tccggagatg
     1621 attcgtggga cgcaggatga cctagtttcg tcacagactc gtctgcaaag gcaagcaaca
     1681 cgggttaaac agcaactcgc cggtgtactt gatactctgc aacagcactt ccagaacatt
     1741 ccacgttcat ccggtcgtca tctttctgta gagaatattg agctggctga tatcggaagt
     1801 gggcgtttca accttcaaat tcgagatgga gaaacattgc ataccacttc tgtggaagta
     1861 ccggaagttg tgtcccgttt tcagaaactt tctaccatgc tttcagccct gcctgccagt
     1921 ggaatcatgg atttcgacct cggcatgagt gtggtcggag tcgtccagta tgcccgcctg
     1981 ctacagcagg ggcacgaaga cagtacccta gccaagataa atctagctat ggatatcaag
     2041 caactttccg aagcaactct cggcagcatg attcagattg ccgggaataa gtttctcaat
     2101 acagaaggaa tccaggggtt cagactggaa agcgccgtcg ctgaaggtat gcgctcagta
     2161 gcaacccgta ccggaggcac aatggggaaa gccctttctg ccagtgcccg tgttcttgaa
     2221 ctgcctgtac tggaaacagt tctggggaca tggaacctgt acaacagcgt cattcagctc
     2281 cagcaagcta catcttattc tgagacaatg gctgcccggg tacagattgc gtttgattcc
     2341 atttctctgg gattaactgc cgcttcggta gctttcccgc cactaattat tgccactggc
     2401 cccattgcgg ctattggtat gggagcttcc agtattgcac gtaatgtggc acggaaagaa
     2461 gaacggcata cacaatggct ggaatataaa aaattcctga ctgatggcag taaacacatt
     2521 gttgtggcct ctccggaaag aggtctgctg gatttctccg gaaacaaagt ttttggaaaa
     2581 atggtgctgg atctgcgtca gtctcctcct ctcttgcatg gagaaagctc ttttaacgct
     2641 gaccgcaaaa tcggtcatcg tccggatctg ggggactggc aaattcgtga gaaggtgggg
     2701 tatgccaaca gtatcagtcc ctactcttct ctggcgcacg gttacgccaa cagtaaatgg
     2761 ccacgaacaa taccgaaaat tccctcggga gaatatgaca ccataattct gggctacggt
     2821 caccagtatc aggccaatac ggaaatagaa tatctgtcta actggattgt atggcgggaa
     2881 gccgtaccag acagtacttc ccgccacaaa cgtcctcctc tggaggttct taatagtcag
     2941 tgtactgtga tagctggaga gcgtaaaacc acagtacttc ccctgagagt gctcagcgat
     3001 ctgacaccgg aatgcacaga acaggctata tcgttaaaag attataaatt catactgaga
     3061 gggggaagcg gtgggctggc tgttcaggtc ggtggcgcgg gatattatga tattgatgca
     3121 aatcctgtgg caaaagaaaa tacgctctct tttcgcgggc taccggaaga gtttccgctc
     3181 acctttgatt tatcaaaaca aacacagtcg gtcatgctga aaacaccaga cgatgaggtg
     3241 ccggtaatga ccattaccca gaagggaata aacaccctgg taggtacagc cgccggtaaa
     3301 gaccgactaa tcggtaacga taaggacaat accttccata caagctctgg cggcggtaca
     3361 gtcatctccg gaggcgggaa taaccgctat attatccccc gggatttaaa aacgccgttg
     3421 acactgacgc tgtccagtaa ctcagtctct cacgaaatct ttctgccaga aacaacccta
     3481 gctgaattaa aacctgtcgc ctttgagctg agtttgattt actgggccgg gaacaacata
     3541 aatgttcaac cagaggatga agcaaaactg aaccactttg ccggaaactt cagggtgcat
     3601 acccgtgatg gcatgactct ggaggcggtt tcccgggaaa atggtattca actggcgatt
     3661 tcattatgtg atgttcaacg ctggcaggct gtttatccgg aagaaaataa cagaccggat
     3721 gccatactgg acaggctgca tgatatgggc tggagtctga caccagaagt ccggttccaa
     3781 ggaggagaaa cacaagtcag ctatgatccc ctgactcgtc agctcgttta ccagcttcag
     3841 gcgcgttact ctgaattcca gttggccggt agtcgccacc ataccacggc tgtaaccgga
     3901 actccgggaa gccgatacat tatcatgaag ccagttacaa cacagatatt accgacacaa
     3961 atcatactgg ctggtgataa tgaccatccg gaaacgattg atttactgga agctagtcct
     4021 gttctggttg aagggaaaaa agacaaaaac agcgtgatat taacgattgc tacgattcag
     4081 tattcccttc aactgacaat atccgggatc gaagaatcgc tgcccgagac aacccgtgtg
     4141 gcaattcagc ctcaggatac ccgtttactg ggtgacgtac tccggatctt accagataat
     4201 ggtaactggg tggggatttt ccggagtggt catacaccaa cggtaaaccg gctggaaaat
     4261 ttgatggcac tgaatcaggt aatgacgttc ctgccccggg tatccggaag tgcagagcag
     4321 gtattatgcc tcgaaaacct aggtggcgta aggaaaaaag tggaggggga gttactgtca
     4381 gggaagctga aaggtgcgtg gaaagccgaa ggtgaaccta ctgttccggt aaatatctca
     4441 gatctaagta tcccacccta ttcacgtctg tatctgattt ttgaagggaa aaataatgtg
     4501 ttgctacgca gtaaagtaca tgcagctccg ttgaaaataa catccgcagg agagatgcag
     4561 ttatctgaaa ggcagtggca acagcaggaa catattattg tcaagcccga caacgaagcc
     4621 ccctcattaa tactcagtga atttcgtcgt ttcactattt catcggataa aacattttct
     4681 ttaaaactga tgtgccatca gggtatggtc cgcatcgacc gcagatcatt atcggtcaga
     4741 ttgttctatc tgcgtgaaca gccaggtatc ggcagtttac gtctgacgtt cagagatttt
     4801 ttcacagaag tgatggatac aacggacagg gaaattctgg agaaagagct gagaccaatt
     4861 ctgataggag atacacaccg ctttatcaac gctgcataca aaaatcatct gaatatccag
     4921 ttaggagatg gcgttctgaa tctggcagac attgttgcgg aatacgcccg tattcaaaag
     4981 gaagaaacat caaaaatatt gtatcaatat caaggtgcca tgaaaaaaaa aacagatgga
     5041 ccatctgtgg tagaagatgc cattatgacc actactgtca caacagattc aggtgaacta
     5101 ttccctacct tccacccgtg gtatacagat gatttatcag ggcgttataa gagcgtacct
     5161 atggcaagaa aagcagatac tttgtatcac ctgacaccaa aaggtgatct acagataata
     5221 tatcaggtag ctacaaaaat ggtgaatcag gcgatgattg tatccctgcc aaactaccga
     5281 cacgagtggg aaaaatataa tttaagcatc ttatccgaaa tccctcagaa caataatact
     5341 gttgtacatt caatcctcag ggttaatggc cccacaatgc aggtgcgcac aattgactac
     5401 agaggaacgg atgaaaacaa tcccatagta tctttttcag atacaacctt catcaatggt
     5461 gaacagatgt tgagttatga ctcgcattca tcagggcgag tctattccag agaagaatat
     5521 atgatgtggg aattgcagca acgggtatca gaagcttcca gtgcccggac acaggattac
     5581 tggctgatgg atgcagcggt aagaaacgga gaatggaaga tcacaccaga attattacgt
     5641 cacacaccgg gatatatccg gagtacggta tcgaaatggt ccagaggatg gctgaaaacc
     5701 ggcacaatac tccagactcc agaagacaga aatacggatg tatacctgac taccatacag
     5761 aacaatgtat ttagtcgtca ggggggcggc taccaagtgt attatcggat tgatggaatg
     5821 gctggtgcgg atatagcgga taatgcacca ggggaaaccc gctgcaccct caggcccgga
     5881 acatgttttg aagtgacaag tgtggatgaa aggcattatg agtggaatat catttatgtc
     5941 acgctgaaaa cctgtggctg gagccgaaat ggccaaagca aaacgccgaa tggtgacaac
     6001 ctttttaact aattaaaatc tctgttaaag atgtataacc cggcatctat gtgctcacca
     6061 gcatatagat gcaatgtgat tcccctgatg gtcgtatcag agatggtatg cgagcctgta
     6121 catagatttg tgtaattgcc tgattttgat atgttcaatc caacatcaaa agcaggttaa
     6181 tttatggacg aaaaacagtt gcaggctctg gctaacgaac tggccaaaaa tctcaaaacc
     6241 cctgaagatc tcagccagtt cgatcgcctg ctgaggaaaa tcagcgttga ggcagctctc
     6301 aacgctgaaa tgtcccacca tctgggctac gataaaaacc agcctaaacc gggtgccaac
     6361 tcccgcaatg gctattccac aaagaccgtt atcatcggtg atggccccct ggaactacgc
     6421 actccgcgcg atcgtgatgg ctcttttgaa ccgcaacttg tgaagaaaaa ccagacccgg
     6481 atcaccggga tggataacca gatcctgtcg ttgtacgcca aaggcctgac aaccgtaagc
     6541 gtacagcctg aaccgtctgg tcagaatctg acgaattaga gtaagcgtaa accgaccgcc
     6601 gtatgtagcc attagacaag aattggtaac ttagacgccc atctgacaca gacggacatc
     6661 taagtatgga attacaggac tggcgaaaag aacctcgtaa aaagtattcg aatgaattca
     6721 aacttcgtat ggttgaactg gcatcacaac ctggagcttg tgttgcacag attgcacgag
     6781 aaaatggcgt caatgataat gttattttca aatggctcag gctctggcag aacgaagggc
     6841 atgtttcgcg gcgtcttccg gtaacaacct cttctgacac tggcgttgaa ttattacctg
     6901 tagaaataac gccggatgag cagaaagaac ctgtggcggc cattgcgccg tctttatcca
     6961 cttccactca gaccagagtc agtgccagtt cctgcaaggt ggaattccgt cacggtaaca
     7021 tgacgctgga aaatccatcg ccagagctgc tcacgatgtt aatccgcgaa ctgagcggga
     7081 ggggaagatg atttcactcc catcaggtac ccgtatctgg ctcgttgccg gcgttaccga
     7141 tatgtgtaaa tccttcaacg gactgggaga acaggtacaa catgtgctga atgataatcc
     7201 cttctccggt cacctgttta tcttccgtgg ccgacggggg gacaccgtta aaattctttg
     7261 ggctgatgct gatggtctgt gcctgttcac caaacgcctg gaggaaggcc agtttatctg
     7321 gcctgcggtg cgtgacggta aggtatccat tacccgctcg caactggcaa tgctcctcga
     7381 taagctggac tggcgtcagc caaaaacatc ccgccttaac gccctgacaa tgttgtaaaa
     7441 aacacctggc cgcattataa aaacggccat gagtcagaaa tacctcattc gcattgcaga
     7501 actggaaagc cagctccgtc agaaagacca gcaactgagt ctggttgaag agacggaggc
     7561 cttcctgcac tggcacgtgc cgaagaaaag atcgaagaag atgaacggga aatagaacat
     7621 ctgcgggctc agatagaaaa actgcgccgg atgctgttcg gtacccgttc tgaaaaactg
     7681 cgtcgtgaag ttgaacaggc tgaggccctg ctgaaacaac gcgaacagga cagcgatcgt
     7741 tacagtgggc gggaagacga tccacaggtt ccccgccagt tgcgacagtc gcgccatcgt
     7801 cgtccgttac cggcacacct tccccgtgaa atacagcgtc tggaatctga agaaagctgt
     7861 tgcccggagt gtggcggtga gctggattat ctgggggaag tcagtgcaga acaactggaa
     7921 ctggtgagca gcgccctgaa agtgatccgc acagaacggg taaaaaaagc gtgtacaaaa
     7981 tgtgactgca tcgttgaagc accggcgccg tcccgcccga tagagcgtgg tatcgcgggc
     8041 cccggattac ttgcccgcgt gttaacggga aaatactgcg aacacctgcc actgtatcgt
     8101 cagagtgaaa tctttgcccg acagggtgtc gaactgagcc gtgcattact ctccaactgg
     8161 gttgacgcgt gctgccagtt aatgacgccg ctgaatgatg ccctgtaccg ttatgtgatg
     8221 aatacccgca agcttcacac tgacgacaca ccggtaaagg tactggcacc gggcctgaaa
     8281 aagacgaaaa cagggcgcat ctggacgtat gtccgggatg atcgcaatgc gggttcgtca
     8341 tctcctccgg cggtctggtt cgcgtactca ccgaaccggc aggggaaaca cccggagcaa
     8401 cacctccgcc ccttccgggg tatcctgcag gcggatgcgt tcacaggtta tgacaggctg
     8461 ttcagtgcag aacgtgaagg tggtgcgctg acagaagttg cgtgctgggc ccatgcccgg
     8521 agaaaaatcc acgatgtata catcagcagc aaaagtgcga cggcagaaga agccctgaag
     8581 cgaatcagtg aactgtacgc catcgaggat gaaatacggg gattaccaga gtcagagcgt
     8641 cttgcagtca gacagcagcg aagcaaagcg ttactgacgt cgctgcatga atggatgatg
     8701 gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg aagcgttcag ctatgtactg
     8761 aatcagtggg acgccctctg ttattacagt gatgacggac tggcggagac ggacaataac
     8821 acagcggaaa gagcgcttcg tgcagtctgt cttggaaaga aaaattacgt gttcttcggt
     8881 agcgatcacg gcggcgagcg tggtgcactg ctatacgggc tgatcggcac ctgccgtctg
     8941 aacggtatcg atccggaagc gtatctgcgc catattctga gcgtactgcc ggaatggccc
     9001 tccaaccggg ttgatgaact cctgccatgg aacgtagtac tcaccaataa ataagcgtca
     9061 atacggtgct ccgttgacgc ttaccttctt tcattcgctg aaggtggaat gtatccacgg
     9121 ggaacgcttt agcagccggg aaataatgcg ggaaacggtg tttaattata tcgagtgtga
     9181 ttacaatcgc tggcgtcgtc acagtgcctg tggcggtctc agcccggact gtcttgcact
     9241 gcaaatattc agaaatgagt ctatgcagtt caccagtaaa atcagccatc agagagggaa
     9301 tggccttatt aatgccctgc cctggtatta atttaaattg taataattta atttcaggat
     9361 gtgtagctgc agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg
     9421 aaagcttaac atttgattct gtatacataa taaatcacct tacagttaca actgtcaaaa
     9481 accgctgtag ccagagttac gctggcctga tgctttagtg tcgagcttcg tcagataatc
     9541 cagacgtgcc aataagcgct gatactgctc agggaaatca ggatcgttaa tatccaggat
     9601 gtcacgtcca ttagcaggga aatgaacaat gcagccccct ggattaacta tgcagaaatc
     9661 gtcctgaggc actgataaat acgagaggac tctcgcgtat ggttgattga caccacagtg
     9721 cgaattcggc gaatgcgaga tcacggtacg attccgcacc acactaccaa tcaatacccc
     9781 gggttttatt caggtaagca ggactgcgga tatccggtgt cgcgcctttg tgtcacgaac
     9841 ggggcaggtg cgaaacaccg gataaaatgc aggttgcgat acctctgaac gccctgcgca
     9901 gagcggagat ttcggattaa gtactcgcac ctccgcagtc ctcaaacaag tctggctggt
     9961 agctgtaaac agacttcgta cataccgctc tgcaataaat ccccgtacca caggcctcac
    10021 agaacttctt cccatgaaaa aactgctcac acatcacaca atgccactcc agcacgaccg
    10081 gtaatggcga taaaaacatc gccatatcct caatgtaagg gtgggacttc tccggattca
    10141 gcaccacaca gtccgccttc tgttgctcgc tcagggcatg taaatcgtgc tcaaaccacg
    10201 ccccctgagc atctgtctgc agaatcaacc ggccatgaca ggaaaggcag aaacactgcc
    10261 ggatatttct gctgagcgca gagtcggcac taataatgtg ctcacccggc gtgatcccca
    10321 gccccgtttt tataccgttc attctgctcc cccctcctca ctgaagcgcc cggtctggca
    10381 gtgtgtgcag taccgctccc cataataatc gtggtgacat tgcctgcagt gccagctggc
    10441 tttacgtact acgggtaagg catccggtac gaattgctgc agacgtttaa tcagttgtat
    10501 ctccttgcgc tccggtctga cataaggaca ctgttgaccg tgctctgtca gcccgtcgtc
    10561 agtgtgttca aaccagggca gttcggtgtc gtattgcggg tggtatctga gtgcactgcc
    10621 acacaagtgg caggtgtagt gggcataagg agcagtatgt gcggtacggg cccccgtcag
    10681 acgtccgtta ccatcaagtg cgagaaagga ttttgcgtac atagtatata ttcctcagcc
    10741 gccagacgac acgcaggcaa cagtcgtccc ggtacgggca gcgtggtcag gtgtgaatgg
    10801 cggttttcag ttcagtctca gaagggaaaa tcagtgtggt cgctgtgaac acgtcatcac
    10861 actaaggtca gataagtcgt ctgtcgggct cagccacgac tgcagccaaa gttgcgctca
    10921 ccggtgccgt aagctttggt actctccttc gccagatagt cagcacgctc cagcacctgc
    10981 tgaaagccgg tgtcatcatc gcgttccagc cacaccgccg gcctgaccgg aaaatgcgcc
    11041 agcgtggtat gatctccggt atccactccc agggcactgc accacgcctg tttaatcatc
    11101 ccggccagtg atcccggagc gcggtaatca ccggcacgac accaggtatc ccggttgacc
    11161 agcagcatca gatgatagtg ttttttacct ttgcactccc caaactcccg ggcccagacg
    11221 taatgcagag tggtgggatg cacgcgttta ccttcccgat gcttacgttt ctggtaagcg
    11281 tcgattcggg ctttcagggc attgatgaag cgggatatca cagctgcgtc cgttgctgcc
    11341 ggtacatccg ggaaacgcag atcaacccgc aatgccgtca ggcggggatg aacattcagt
    11401 gcgtgccgca ccgtctcacg aatacgttgc tgccagaagg ggttgtattt gtaggtcatg
    11461 gttaaatctc cgtatggttc atacggaata gccacgtcgt aaaaaatgcg cagagtccct
    11521 gaagtgacct ccggcaggac actgtctcag gcactttgtg ataacccgaa cggtcgtctc
    11581 cggactgacg gttgaatttt cagcgttgtt tttttaatgt aaaaaaccgg ttacgggtaa
    11641 ggctgtgagg agggagtgat ggtgatacgc aaaaagaagt gcagggactg tggtaacgcg
    11701 attactcata atacggtgtg ttgtccgtac tgtggttctg tcgacccctt cggctattac
    11761 cggaatacgg acaggatctt cacagccctg ctggcgctgc tggttgtggt tttgctggtg
    11821 acatctgctg tcagcatgta tgtgctgtgg tagcggtaac ggggcttatc agatatccgt
    11881 gagggttgcc agagttcgtc ccctgcaacg cgccgtcaac ccacacgctg taagccgt
//
LOCUS       DAZGCS010000132        11911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGCS010000132 DAZGCS010000000
VERSION     DAZGCS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.134"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..546
                     /gene="tssB"
                     /locus_tag="ACHK01_004211"
     CDS             37..546
                     /gene="tssB"
                     /locus_tag="ACHK01_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8099058.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKL
                     RRRRTFNPK"
     gene            595..2133
                     /gene="tssC"
                     /locus_tag="ACHK01_004212"
     CDS             595..2133
                     /gene="tssC"
                     /locus_tag="ACHK01_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8099059.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFSNLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWVRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2151..3488
                     /gene="tssK"
                     /locus_tag="ACHK01_004213"
     CDS             2151..3488
                     /gene="tssK"
                     /locus_tag="ACHK01_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8099060.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALMSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLHALTNDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3485..4150
                     /gene="tssL"
                     /locus_tag="ACHK01_004214"
     CDS             3485..4150
                     /gene="tssL"
                     /locus_tag="ACHK01_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO8099061.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     GVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQAHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4163..5815
                     /locus_tag="ACHK01_004215"
     CDS             4163..5815
                     /locus_tag="ACHK01_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8099062.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLIWAGAAAIVLTVA
                     ALLIYRRRQAWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQAERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEIHVATSAETASPAQWLTQTSTQE
                     RSQQLCYLLKAESLMQWLNLNMLAALNGPETKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATENRQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAIMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5873..6364
                     /locus_tag="ACHK01_004216"
     CDS             5873..6364
                     /locus_tag="ACHK01_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8099063.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFIFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6555..9200
                     /gene="tssH"
                     /locus_tag="ACHK01_004217"
                     /gene_synonym="clpV"
     CDS             6555..9200
                     /gene="tssH"
                     /locus_tag="ACHK01_004217"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8099064.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLTALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQATEPLTSAQPESATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTASLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLQARLTALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKRL
                     AQQIMALRQEGADSTDLQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9212..11719
                     /locus_tag="ACHK01_004218"
     CDS             9212..11719
                     /locus_tag="ACHK01_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8099065.1"
                     /translation="MGIQNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
BASE COUNT         2799 a         3263 c         3462 g         2387 t
ORIGIN      
        1 taaccatcag aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa
       61 gttcccgccg ctcgtgtaaa tatcaagctt gacctgcata caggcaatgc taaaaagaaa
      121 gttgaactcc ccctcaagct tcttgctgtt ggcgattaca gtaacggaaa agagcaacgt
      181 ccgctgtccg aacgggacaa gattgatatc aataaaaaca acttcaacag tgtcatggct
      241 gagttttcgc ctgcggttaa tttaacagta gaagacacac taagcggaag cggtaatgag
      301 caaaatatcg cgcttgaatt taaaagcctg aaagattttg aacccgaaca ggtagcaaaa
      361 aatatcccac agcttcgtgt attactcgct atgcgcaatt tattacgtga cctgaaatcc
      421 aatctgctgg ataacgcaac gtttcgccgt gaactggaaa atattctgaa agacccaacg
      481 ctcagttcag aactgcgtga tgaactggcg aaattgcgcc gcaggagaac gtttaacccg
      541 aagtgatatt caggttaaac cggagaagaa aggatcactg aaaaggaaat gctgatgtct
      601 gtacaacaag aacatgccac ctctgaaact gcaacactga ccaccactga gtccggcggc
      661 gtttatcagt ccctgtttga taaaattaat ttaaccccgg tgtcttccat tcaggaaata
      721 gatttatggc aaaacagcga aacactggcg gatgcctcac ccgatgagcg cgtgacagcg
      781 gcgattcacg ttctgctttc ctgtctggcg aaatcaggcg agaacgtggt caagctcgac
      841 aagagcctgc tggattttca tatcgacgat ttggatcaga aaatcagtaa gcagcttgat
      901 gcggtcatgc accatcctga attccagaaa gtcgagtcgc tgtggcgcgg cacatggttc
      961 gtcgtacagc gcactgattt tcgcaaaaat gtcagaattg aattgctgga tatcagcaaa
     1021 gagcatctgc gtcaggattt cgacgactcc ccggaaatca ttcagagtgg tttatatcgt
     1081 catacatata ttcaggagta cgatacgccg ggtggcgaac ctgttgcctc attaatttcc
     1141 agctatgaat ttgataacag cccgcaggat attgccctgc tgcgcaatat ttccagagtg
     1201 tctgccgctt cccatatgcc ttttatcggt tctgtcgggc cgaaattctt ccttaaaaat
     1261 tcgatggaag aagtcgccgc gattaaagat atcggcaact actttgaccg cgcagaatat
     1321 attaaatgga aatcgtttcg tgataccgat gacagccgct atgtgggatt agtgatgccg
     1381 cgagtgctgg gccgtctgcc ctatgggccg gacacggtgc cggtacgcag ctttaactat
     1441 gtggaagaag tcaaaggccc ggatcacgaa aagtatctct ggacaaacgc ctcgttcgcc
     1501 tttgccgcca atatggtgaa gagctttgtg aataatggct ggtgcgtgca gatccgcggt
     1561 ccacaagcag gtggcgcagt ggccgatctg cccatccatc tttacgatct cggcaccggc
     1621 aatcaggtca aaattccgtc cgaagtgatg atcccggaaa cccgcgaatt tgaattttcc
     1681 aaccttggct ttattccgct ctcttattat aagaatcgcg attacgcctg cttcttctcg
     1741 gcaaactctg cccagaaacc ggcgttgtat gataccgctg acgccaccgc caacagccgt
     1801 attaacgccc gtctgcccta tatcttcctg ctgtcccgca ttgcgcatta cctgaaaatc
     1861 atccagcgcg agaatatcgg taccaccaaa gaccgccgcg tactggaact ggaactgaac
     1921 acctgggtcc gcacactggt gacggagatg accgatccgg gtgatgaact gcaggcgtct
     1981 cacccgctgc gcgacggtaa ggttatcgtg gaagatattg aggacaatcc gggcttcttc
     2041 cgcgtcagac tctttgccgt gccgcatttc cagattgaag ggatggatat caacctgtca
     2101 ctggtttccc agatgccgaa agcgaaagcc tgaccgggaa gtggtgtgat atgaaaattc
     2161 atcgaccact gtgggctgag gggacattcc tgtcctctca gcagtttcag cagcaggccc
     2221 gctgggaagc gttcagcaat gacagcattg cgcaactttg tattcgtcat ccgtggggga
     2281 tcgcgaatgt gctctttgac cgcgatgccc tgatgtccgg aaagctgaaa acgcaggcag
     2341 tacgcctgcg ttttgctgat ggcacgctga tcgattctga cgtgagcgat gttctgcctc
     2401 tcgcctgcga tctgcacgcg ctgacgaacg acagcgcggt tgttctgctg gcgctgccgc
     2461 ttgcccatgg taacggcggt aatctcggtc agggtgagca gactgaaaga ccgctacgct
     2521 atcgtcagga atggcaaaag gtacaggata tctacggcag tgacagtgaa gatatggccg
     2581 ttgagcgtca tgcgctgtcc ctgcgctttg ctcatgataa caatcaggat tacatcacct
     2641 gcccactggc gcgtctggtg cgggatgttc agggcaactg gacacaggat gagggctata
     2701 ttccgccgtt actggcgttt aatgctcatg acgggttggt acagcgtctg gacacgctgc
     2761 tgttgcagct acgggccaaa tgtcagcgcc tgatggcgat gcgtcgggaa agcaaccagc
     2821 gtatggcgga cttcgccgtc gccgatgtct ccctgttctg gttgcttaac gcgctgaact
     2881 ccgccgagcc ggtgctgagt gatttcctgc gctacccggc ggttcatccc gaactggtct
     2941 ggcgggagct ggcgaggctt gccggagcgc tgctgacgtt ttcccttgaa cataacgtca
     3001 gcgccgttcc gccctatgtc cacgaatcgc cgtccactgt ctttccgcca ctgttctccc
     3061 tgctcagcga actgctggaa gcaagcctgc cgtcacgggt gatcgcgctg gatctgggat
     3121 ccctgccggg caaccgctgg aaagccgacc tgcacgatcc gcgcctgcgg gaagaggccg
     3181 atttttacct gtccgttcgt tcctcactgc ctgcgcatca ggtactgcac cagctaccgc
     3241 tggtctgcaa aatcggtgcg cctgatgacg tcacgctgct tatcaacgtg gcgctgaatg
     3301 gtgttccact ggtgccactg acctcagttc cggcagcgct gccactgcgc ctggagaatc
     3361 agtattttgc gctggatatg cacagtgacg cggctaaatc gatgctggag tccgggtgct
     3421 gcatgattta tgcgccagga accatgggcg acctgaaacc tgaactgttt gcggtgctgc
     3481 gcacatgaaa aaagagacgg atatcaatat cgacgcgctg ctgcgcgaca cgtttctgac
     3541 ggtggtggaa ctgcgtcagg gaaccaccgt gcgtcacggt atggagctgt atcgccactg
     3601 ccagcggcag gtggaggggg tccgcgaacg cctgaaagat gccgggttca gccgggagaa
     3661 cgtggagcac attacttacg cacagtgcgc cctgctggac gaaacagtcc tgagtcgcag
     3721 cggcatggac gacggtcagg ccatctggat gaaggatcca ctccaggcgc atttcttcaa
     3781 caccctgcag gcaggcgagc tgctgtacga gcggatgaag caggtgttgc aggagcccgc
     3841 gcctgcacag gcagtgctga cctgctttca ccgtgtcctg ctgctgggat ttcgcgggcg
     3901 ttatcaggat ccggtggccc cggagcgtga ccaacttatt tcgacgctca acgggctggt
     3961 cgcgccattt ggcgtgctgc cggaaacggc ggtactgaac gtgccgttat ccccccgtca
     4021 gcaccctttg ctgcattcgc cgtttttctg gctggtaacg ctgacgctgc tactggccgg
     4081 tgtctggtgg ggactgcacc actggctgaa cgtgctggtg gatgaattgc tgccgcaaag
     4141 cctgcggtaa agggagtcgt gagtgaggaa cacgctgaaa caggccgtcg tgctgtgggg
     4201 aatggtgtta ctgctggtgc tgtggtcggt gtttatcagt ccgtctggcg tactgatatg
     4261 ggccggggcg gcggctatcg ttctgacggt tgccgcgttg ctgatttacc ggcgcaggca
     4321 ggcgtggacg gagatgaccg gcgatgccgg gttgttatcg ctgccgccgg aaacgtaccg
     4381 acaaccggta gtgctggtct gtggcgatat gtcggcgcat ctgttcactg acagcccggt
     4441 ccggcaggtt tcagaagggc tgtatctgca tgtttctgat gaagaacagc ttgtggcgca
     4501 ggcggagcga ttactgaccc ttcgcccggc gtgggcatcg cagcttgccg tggcatatac
     4561 cgtcatgccc ggcatgtacc gggatgcggc ggttctgacc ggacaactgc gccggttcgc
     4621 ccacagtatg gcgacagtgc gtcgccgggc gggcgtaaac gtcccctggc ttctctggag
     4681 cgggctgtcc ggctcgccgt tgccggagag agcgaattca ccgtggttta tctgtaccgg
     4741 cggcgaaatt catgtagcga cgtctgctga aaccgcctcc cccgcgcagt ggctcacgca
     4801 gaccagcaca caggagcgca gccagcaact ctgttacctg ctgaaagccg aaagcctgat
     4861 gcagtggctg aatcttaata tgctggcggc actgaacggc ccggaaacga aatgtccgcc
     4921 actggcgatg gccgtggggc tggtcccctc gttgcctgcg gtggataaca acctgtggca
     4981 gttgtggatc accgccagaa ccggcctgac gacggatatc gcggacaccg gcacagacgc
     5041 cacgctgccg tttccggatg ccctgttacg gcggttgccg cgtcagtcgg gctttacccc
     5101 gctgcgacga gcctgcgtga ccatgctggg catcaccacc gtggcgggta tcgccgcgct
     5161 gtgtctgtca gccacggaga atcgccagtt attacggcat atcggtgatg atctgcacca
     5221 gttttatgcc gttccggcgg aggagtttat caccaaagcc cgtcgtctgt cggtgctgaa
     5281 agacgatgcg atcatgctcg atgggtatta ccgggaagga gagccactgc gtctcggtct
     5341 ggggttatac cccggtgaac aaatccgcca gccggtatta cgcgccattc gtgactggcg
     5401 tccgcctgag caaaaaatgg aagtgacagc ctcgcttcag gcgcagaccg tgcgtcttga
     5461 cagtatgtcg ctgtttgacg tcggacaggc ccgcctgaaa gacggctcga caaaagtgct
     5521 ggtggacgca ctggtgaaca tccgggcaaa accgggctgg ctgatcctcg tggccggata
     5581 taccgacgcc accggcgacg aaaaaagcaa tcagcagtta tcgctgcgac gtgccgaagc
     5641 ggtgcgcaac tggatgctgc agaccagcga catcccggcc acctgttttg ccgtacaggg
     5701 actgggcgag agccagcctg cggcgaccaa cgatacgcca cagggccggg cagtcaaccg
     5761 gcgcgtcgaa atcagtcttg ttccgcgttc tgacgcctgt caggacgtga aataaaacat
     5821 accgccggaa gaaggcggtg cttcaatcac actaacaagg agagtaattc tcatggctat
     5881 tcctgcttat ctctggctga aagatgacgg cggcgcggat atcaaaggtt ccgtggacgt
     5941 tcaggggcgc gaaggcagca tcgaagtggt ggcgctggat cacgatgtgt acatcccgac
     6001 cgacaacaac accggcaaac tgaccggtac tcgcacccat aaaccgttca tttttaccaa
     6061 agaaatcgat gcgtccagcc cgtatctcta caaagctgtg accaccggtc agaccctgaa
     6121 aacggcggaa tttaagtttt accgcatcaa cgacgccggt caggaagtgg agtacttcaa
     6181 catcacgctg gataacgtca agctggtcag agtcgctccg ctgatgcacg acatcaagga
     6241 gccttccaaa gagaagcata accacctgga acgcattgag ttccgctacg aaaaaatcac
     6301 ctggacttac aaagacggca acatcattca ttccgactcg tggaatgaac gtccttccgc
     6361 ctgataactg cggcagacag gaaactgtct gcctttctgt tgtgtgcttt gcccagtgcc
     6421 gcaggcaccg gtactgagca aagcataccc acaatctggt gatacctgat gggcctgagt
     6481 gaggtaacgg ctatgggcgg tagcgtacct gaacgcagat cgcaggaata cagaacacga
     6541 taaggaaacc ttctatgacc ggaaatcacc ccgccgcgct gctgcgtcgc cttaacccgt
     6601 actgtgcacg ggcgctggac gctgccgcct cactgtgtca gacccgcgcc catgcggaga
     6661 taaccattga acactggctg ctgaaactgc tggagcaggg agaaggcgat atcacggtga
     6721 ttgcccgccg ctatgaatgg gatatcgaca cgctctggca gtctctgctg gcacatctgg
     6781 acaccttacc ccgctcggtc cgcgaacgtc cgcagctttc tgaacccctg acggcgctta
     6841 tcaggcaggc gtggctgatt gcctcgctgg aaggcgacga cccgcaaatc cgcagtcagc
     6901 acctgctgat ggcgctgaca gaaaaaccga tgctgcccgc ctgtaatgac ctgtgggtat
     6961 tgctgagcct gagccgcgtg cagcttgagc ggctgcgccc cctgctggat gcgcagtcgg
     7021 atgaatgtcc ggcacgtcag ccacaggcca ccgaaccgct gacatccgcg caaccagagt
     7081 cggcaacgac ggacgcaccg gcaaaaacgc tgacggggaa acaggatgac gccctgctgg
     7141 cggtacttaa ccgctttacc gaagacgtga cggaaaaagc ccgcagcggg cgaatcgacc
     7201 cggtattcgg gcgcgacacc gaaattcgcc agatggtcga tatcctctcc cgtcgccgca
     7261 aaaacaaccc gattctggtg ggagaaccgg gcgtgggcaa aaccgcgctg gtggaagggc
     7321 tggcgctgcg tatcaccgaa ggcaacgtgc cggacagcct gaaaacggtg catatccgca
     7381 cactggacct cggtctgtta caggctggcg cgggcgttaa aggtgaattt gagcagcgac
     7441 tgaaaaacgt gattgatgca gtacagaaat caccggagcc ggtactgctg tttattgatg
     7501 aagcccatac cattatcggc gcgggtaatc aggcgggcgg cgcggatgcg gcgaacctgc
     7561 tgaaaccggc gctggcaagg ggcgagctgc gcaccatcgc cgccacaacg tggagcgaat
     7621 acaaacagta ttttgagcgc gacgccgcgc tggaacgccg cttccagatg gtgaaagtcg
     7681 acgagccgga tgatgacacc gccagcctga tgctgcgggg actcaaggcc cgctatgcac
     7741 agcaccacgg cgtacatatg ctggacagcg ccattcagac cgccgtgcgc ctgtcgcgcc
     7801 gctatctgac cggacgccag ttgccggaca aggcggttga tttgctggat accgccgggg
     7861 caagagtccg catgagcctc gacaccctgc cggaaccgtt gacgcagctt caggcgcgtc
     7921 tgaccgcgct ggatattgag cgggaagcga ttgagcagga cagcgtgttt tatcccgaaa
     7981 ccagcccgga gcggctggcg gaactgaccg atttgcgtga tgagctacag gcagaagccg
     8041 ggcatctgga agcgcagtat cagcaggaaa agcggcttgc gcagcagatt atggcgttgc
     8101 gtcaggaagg ggcagacagc actgatttgc aacagcaact gcgaacgcat cagggctttg
     8161 caccgctgct ggcgctggat gtggacgccc gcgccgtcgc cactgtggtg gcggactgga
     8221 ccggcatccc gctctcttcc ctgctcaggg acgagcagag cgacctgctc agtatggaac
     8281 aaagccttga aaaccgcgtt gtcgggcaaa gcccggcgct ctgcgccatc gcacagcggc
     8341 tgcgggcggc taaaaccggc ctcacgccgg agaacggccc gcagggggta ttcctgctga
     8401 ccggccccag cggcaccggt aaaaccgaaa ctgcgctcac actggccgac accctgtttg
     8461 gcggtgaaaa atcccttatc accatcaacc tctccgaata tcaggagccg cataccgtct
     8521 cccagcttaa ggggtctcct ccgggctacg ttggctacgg tcagggcggc gtactcaccg
     8581 aagccgttcg caaacgcccg tacagcgtgg tgctgctcga cgaagtggaa aaggcgcatc
     8641 gcgacgtgat gaacctgttc tatcaggtat ttgatcgggg ctttatgcgc gacggcgaag
     8701 gccgggaaat tgacttccgc aacaccgtga ttctgatgac ggctaatctg ggcagtgacc
     8761 acatcatgca gctgctggag gaaaaaccgg acgccacgga cgcagacctg catgaactgc
     8821 tgtaccccct gctgcgcgag cacttccagc ctgcactgat ggcgcgtttt cagacggtga
     8881 tttaccgccc tctgggacag gaagcgatgc gcgccattgt ggaaatgaaa ctggcgcagg
     8941 tggttcgtcg tcttcgtcag cactatgggc tggaaacgga aatcaacgac agcctgtatg
     9001 acgcgctgac cgccgcctgc ctgctgccgg acaccggggc gcgtaatatc gacagcctgc
     9061 tgaaccagca aatcctgccg gtcttaagcc agcagttgct ggcgcaacag gccgcgcacc
     9121 gtaaaccagc ccagctacgg cttggctggg atgaggaaga cgggattgta ctggagttcg
     9181 ctacggaaga gatgcaataa cggaggctgt catgggtatt cagaatttcg atcattcaca
     9241 ccacaagctc aaaattcgtg gcctgcaaag tccggtggat gtgctgacct tcgaaggccg
     9301 cgaacaactg agtaccccgt tccgctacga cattcagttt accagcagcg ataaagccat
     9361 cacgccggag tcggtgctga tgcaggacgg cgcattcagc ctgacggcac cgccggtaca
     9421 ggggatgccg gtacagactg ccctgcgcac cctgcacggc gtcatcaccg gctttaaaca
     9481 cctgtcatcg tcacaggatg aagcccgtta cgaagtgcgc cttgaacccc gaatggcgct
     9541 gctggcccgc agccgccaga acgccattta ccagaaccag acggtgccgc aaatagtcga
     9601 aaaaatcctg cgcgagcggc accagatgcg cggtcaggac ttcgtgttta acctcaaaag
     9661 cgaatacccg tcccgtgaac aggtgatgca gtacggtgag gacgacctga cgtttatttc
     9721 acgcctgctg tcggaagtgg gtatctggtt ccgctttgcc accgacgccc ggctgaaaat
     9781 tgaggttgtc gagttttacg acgaccagag cggctatgag cgcggactga cgctgccgct
     9841 tcgccatcct tcagggctgt ttgatggtga aacggaagcc gtatgggggc tgaacaccgc
     9901 atacagcgtg gtggaaaaaa gcgtcaccac gcgggactac aactaccgca ccgccacagc
     9961 agagatgatg acagagcagc atgacgccac cgggggcgat aacaccacct atggcgaagc
    10021 ctaccactac gctgacaact tcctgcaaaa aggcgacaag gaggcagcgg aaagcggcgc
    10081 gttttatgcc cgtatccgtc acgaacgcta cctgaatgaa caggccattc tgcagggcca
    10141 gagcaccagt tcactgctga tgccggggct ggaaatcagg gttcagggcg acgacgcgcc
    10201 tgcggtattc cgcaagggcg tgctgattac cggcgtgacg gccagcgcgg cccgtgaccg
    10261 cagctatgaa ctgacgttca cggcgatacc ctactcagaa cgctacggct atcgcccggc
    10321 gcttatcccc cgccccgtaa tggccggaac actcccggcg cgggtcacca gtacggtgaa
    10381 gaacgacatc tacgcccata tcgataagga cgggcgctac cgggtcaatc tcgactttga
    10441 ccgtgatacg tggaagccgg gctacgaaag cctgtgggta cgccagtcgc gcccgtatgc
    10501 gggagacacc tacggcctgc acctgccgct gctggcggga acggaagtca gcatcgcctt
    10561 tgaggaaggc aacccggacc gaccgtatat cgccggggta aaacacgact cggcacacac
    10621 cgaccatgtg accattcaga actacaagcg caacgtgctg cgcaccccgg cgaacaacaa
    10681 aatccgcctc gatgatgagc gcggcaaaga gcatatcaag gtcagtaccg agtacggcgg
    10741 caagagccag ttgaatctgg gtcatctggt ggatgccgga aaacaacagc gcggggaagg
    10801 ctttgaactg cgtaccgata tgtggggcgc ggtgcgggcg aagaaaggga tttttatcag
    10861 cgccgatgcg caggataagg cgcaggggca ggtgcgcgaa atggctccgg cgatggcaat
    10921 actggatggc gcacagtcac agatgcagtc gctgtccact gatgcgcaga cgacgaatgc
    10981 cgatccggca gatctgtcat cgcagatagc gctgttgcag caaagtgtaa aagatctgac
    11041 gcaggccgtg atcctgctaa gtgcacccaa gggcgtggcg atcgccagcg gcgagcacct
    11101 gcaactggcg gcgtcgaaga atgtgatagc gaacgcaggc aacaacgcag atatcggcgt
    11161 agtaaaaaat atgtttatcg gtgtgggtca ggcgctgagc gtgtttgtgc gtaaagcggg
    11221 catcaagctg tttgccaata aaggggcggt ctcggtacag gcacagaacg atctgatgga
    11281 actgttggca cagaaatcga tagagatcac cagtacggaa gatgagatca agatcaccgc
    11341 gaagaagaaa atcaccctta acggcggtgg cagctacatc cggctggatg cctgcgggat
    11401 agaagcggga acgccgggag aatataacgt taaggccgga tattacgggc gtaaaccaaa
    11461 ggcgaagctg acgccggagc tgatggcatt tccggtgatt aagtctgagg attttaatca
    11521 gagctttatt ttgctggatg aaaatactgg gcaaccactt attaattggc cctatgaact
    11581 agaactggaa agtgggctaa aaatgagtgg gattactgat gaaaatggaa atacagaact
    11641 aatatcctct gataaggagg aggtcgttaa tattagtgtg tttgaacccg atgagttttt
    11701 agacgatgac attaattaag acatatagcg agaatataac atggcagata aatccgaaaa
    11761 gaaaaaagaa accacaaata aatcagcaaa aaaaacacta acgaagaaga gcgaaaaatt
    11821 aactccagca aaagacaccg caccaaaaca agtgaatgta gatccagcta taagattgaa
    11881 acaaattcgt caacttgtcc gtaataataa t
//
LOCUS       DAZGCS010000133        11894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGCS010000133 DAZGCS010000000
VERSION     DAZGCS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.135"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..484
                     /locus_tag="ACHK01_004219"
     CDS             <1..484
                     /locus_tag="ACHK01_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO8099066.1"
                     /translation="PDFNYIYQNYGDDFVTHTAKFSGETVERAIERAIVSKLNIVVEG
                     TFRNAATPLQTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAGNIARKVDKN
                     HHDIVTGLLAENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLIQNEISGNTQ
                     "
     gene            complement(520..1311)
                     /locus_tag="ACHK01_004220"
     CDS             complement(520..1311)
                     /locus_tag="ACHK01_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO8099067.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1317..2444)
                     /locus_tag="ACHK01_004221"
     CDS             complement(1317..2444)
                     /locus_tag="ACHK01_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO8099068.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWFTTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            2973..3245
                     /locus_tag="ACHK01_004222"
     CDS             2973..3245
                     /locus_tag="ACHK01_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO8099069.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDHARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDTEASFNWSANTPTHGRR"
     gene            complement(3246..4118)
                     /gene="rluF"
                     /locus_tag="ACHK01_004223"
     CDS             complement(3246..4118)
                     /gene="rluF"
                     /locus_tag="ACHK01_004223"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO8099070.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPLVKMEKTSEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            4330..5019
                     /gene="pepE"
                     /locus_tag="ACHK01_004224"
     CDS             4330..5019
                     /gene="pepE"
                     /locus_tag="ACHK01_004224"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO8099071.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5110..6741)
                     /locus_tag="ACHK01_004225"
     CDS             complement(5110..6741)
                     /locus_tag="ACHK01_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO8099072.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGGEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6961..10644)
                     /gene="metH"
                     /locus_tag="ACHK01_004226"
     CDS             complement(6961..10644)
                     /gene="metH"
                     /locus_tag="ACHK01_004226"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO8099073.1"
                     /translation="MSSKVEQLRAQLNQRILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            10844..11668
                     /gene="iclR"
                     /locus_tag="ACHK01_004227"
     CDS             10844..11668
                     /gene="iclR"
                     /locus_tag="ACHK01_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO8099074.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDSVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2857 a         3260 c         2921 g         2856 t
ORIGIN      
        1 ccctgatttt aattatatct accaaaatta tggggatgat tttgttaccc atacagcgaa
       61 attttccggc gaaacggtcg aacgtgcaat tgagagagcg atagtcagta aactcaacat
      121 tgttgtcgaa ggaacatttc gtaatgccgc gacgccgtta caaacattaa aaaaactcaa
      181 agatgcaggc taccaaacag aagtcatgat aaaaacaacc tcagcggcat taagctggga
      241 aagcaccaat gaacggtata gtaaggacaa agaggcggga aatattgccc gaaaagtgga
      301 taaaaaccac catgatatcg tcaccggttt gctggcagaa aatgcccgca aggtgtttgc
      361 ttcgaacctg tcagataagt ttgcggtgta ttcgcgtgag aagatgatat ttagttcaca
      421 agcggcaacg aatgacgata ttgcaacact tatacagaat gaaatcagcg ggaatacaca
      481 ataataataa gagctgatgg cgtgaaataa cgccgggctt tatgcccggc gcattaccgt
      541 ctggtacatt aagctatcca gttccttaac gtccccttct ctgctatctg gtaacagcgg
      601 tggaatttcc ccacgtgcct gttccgcacg aataaacgca tgtaacaatg gacggcgcgg
      661 accatattcc gcttctcctg cgcgctgttt acgttccagt aattcgtcta tttctgcacg
      721 taacggcgca tcaagttcgc ttcccgccag taactcagca aagcgcattg gcggtacgcc
      781 tttacccgct tcgacccagc gcacggccag cagcggacgc agcacgtaaa agtatttttt
      841 cagccgcact tcgtcgcctt gtaaataacc gcgaaagttt ttccgcgcca tcgaataata
      901 atgccaacgc gcacgcagcg gagaaaacca cttcggcacc attgccttaa gcgccgttat
      961 cgtttcttca tcttgttgat agaccacagg agaatccagc cattctatca gcgtcggatt
     1021 cgcacctttc aataatccca gcgccttgcg ccactcccag cctgaaacat ctaactcgtc
     1081 gtcaataggc aactcaataa catcgcgcgg cgcctctact cgcagatacc attccagcgg
     1141 gtgaacatac aaaaaccgta catcgtagtc gctgtcgggc gaggcaaatc cccagccgcg
     1201 gctccctgat tcacaggcgt acagtacttt cacatcatac cgccgttcaa tttctcttaa
     1261 ctgctgcgat accctttctc gcatcgcagc actgactcca tttaatggca tgttactcac
     1321 cctttgatat taaccaaccc atgcagaacc tgatccagac cgccaaatac agagatgtta
     1381 tcgatccgtt cagccactct ctccagcgtt tccagttctt ttaagcgtaa cgctaccggg
     1441 ttgttttcca tgactttagc ggtattgagt aacgaacgcg tcgctgccgt ttcttcacgt
     1501 cggcgaatca cattggcctg cgcggatttc tccgcctcaa ccagctgcgc aaggatattt
     1561 ttcatatcgc caggcaggac gatatctttc acccctaacg aggcaatctc cataccaaat
     1621 ggcaacatgc gcgatttcac ctgttcgctg acaacgtcat caatgacctg tttatcttcc
     1681 agcaattcat ccaacgtacg cgtacctacc gcttcacgca gggcaaattg taattcccga
     1741 tacaaatgat caatcggctt cgttaactgg ctaaatgcca ataacacgtc gctgtagcgc
     1801 cagttagcgg cgaggttaat ccgcagattg accttatctt tcgttaagat ttcttgcccg
     1861 ctgacttcca gtacctgcaa gcgggtgtca accacttcag cgtcaaccag atggttaatt
     1921 ttccagtatg cggtcagccc cggcggtaac agcgcctgcg tttcaccatc gattttcagc
     1981 acgcctacat gccaggccgg aacctgcacc gttagtattg catcgcggcc tttaactgcc
     2041 cctttgcgcc gtggttgcaa taccgcattc atcacctccg tttgtacctg gacttgctgc
     2101 gtattcatcc gcaccagtgt caaatcatcc tctacacgcc agtacaaacg gcgcgttgat
     2161 ggcggcagga tctcctgcaa aataccgtcc atatacaacg ccccggcttc agtctccgac
     2221 aagtcggcca ccacgcaata tctttccacc caatcaggct gaaagcggcg caaataatcc
     2281 gccagcacgt cgggcacttc gctaccatcc agcgtaatca gcaacacttc cggggtagta
     2341 aaccagggta aaatatgctc gcctgcatgt agcactttgt agtaatcgcc gtttctggag
     2401 agtaatgcca gctgatcttt tcttacgctt attttcttaa tcattatcta ttccttgctt
     2461 aacagccgtc gcggaagcgg acacgaagcg acaggggaat tcccttttgc ctgttacccg
     2521 gcgtctttat cggcgacgtt atcggggatc gctgactgcg caatccctca ttttttattt
     2581 aataagttac aaccagtatt catttcagga atgaatgtgg gaatcgaacc cataattggc
     2641 gtcaaaggct gacggaacac gcccggaaaa cagcctgacc gttcctggtg tgccgacggg
     2701 gtttaacccc ctttgaaatt cagtatcaac gcaaaagtgt tattgcgaga agcgtgccag
     2761 aattgagaaa aattcttaac caaatgaaaa ataaagagtt agtgaattac ttttgtttgt
     2821 agggggaaaa cttttcgcta aatgtgtatc tgtatttatc gtcaattatc aagaaagata
     2881 aatatttaag aagagaacaa aggaatatta tcagttctgc ttgcaaatga attcccgtac
     2941 actgaatcca tcggctaact gagggcaaca tcatggcact cccgcgtatt acccaaaaag
     3001 agatgacaga gcgcgagcaa cgcgagctaa aaacgctgct ggaccacgcg cggattgcgc
     3061 atggtcgcgt actgaccaac tccgaaacca acagcattaa aaaagagtac atcgataagc
     3121 tgatggttga gcgtgaagca gctgcaaaaa aagcccgcca gttgaagaaa aagcaggctt
     3181 ataaaccaga tactgaagca tcgtttaact ggtcggctaa tacaccaacg cacggcaggc
     3241 gctgattaac gccccttctt tttacgcccc ggcgatgtga aacgtttacc atttgacgcc
     3301 gggcggccac ctttttctga tgttttttcc atcttcacca acgggcgttt gatgcccgcc
     3361 gttttcggtt tggccttcac tttaggtttt gcctcggaag aggaattttc gatgagctta
     3421 aacaggtcga ttaactcatc gtcagttaaa tcgcgccact cacccagcgg aatgccgctt
     3481 aagctgacgt tcataatgcg cgtacgttcc agctttttca cttcatagcc gaaatgctcg
     3541 cacatgcgcc ggatctgacg gttcagcccc tgcaccaggg taatgcggaa gacaaacggc
     3601 gcttcttttt tgactttgca ctttttcgtc accgtcccga ggatcggcac ccccgccccc
     3661 ataccacgaa taaattcatc ggtaatcggt ttatcgaccg tcaccagata ctctttctca
     3721 tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt gaggaaaatc
     3781 agcccctggg agtctttatc caggcggcca atcgggaaca cgcgtttgct gtggttaacg
     3841 aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc gacgggcttg
     3901 ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg accgtttact
     3961 ttcacaacgt cgccgggttt cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac
     4021 acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg
     4081 atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt aaaaaacgga
     4141 atataccgta cctttgggtt gataaaaaat agtcatgagc gaactatttg ttatttccat
     4201 tggcttgaat tcgttacctg ctcaaatcac tttccagaag caatgtgcga catcgtgagc
     4261 catgtcagta agtggttaaa ttcgcagtgg tagcatgcct cccgtgattt cttactaacc
     4321 ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg
     4381 gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc ggtgtttatc
     4441 cctttcgctg gcgtaacgca gacctgggat gattacacag cgaaaacggc tgcggttctc
     4501 gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt tgccgcgatt
     4561 gaaaatgctg agatcgtgat tgtcggcggc gggaatactt tccagttgct gaaacagtgc
     4621 cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggcgc tctgtatatt
     4681 ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg
     4741 attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc cgttgcaaat caacccgcac
     4801 ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa
     4861 ctgctggtcg tcgcgccaga actgacgatt attggtctac cggaaggtaa ctggatcaca
     4921 gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt taaggctggt
     4981 gaagaagcgg tgccgctaga agctggtcac cgtttttaag tgaattgccg gatgcatgat
     5041 ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag gcctacagga
     5101 ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca gcacactgta
     5161 agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc ctaaatgtag
     5221 cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt gggcgtggga
     5281 atagcggcga ttaagaatgc gaaagcgatg cttactacgg cgcaaacgac gagcgctggt
     5341 gacatcgcca gagaagaaca ccgacattgc cagttttaaa ttactgagca attgctcata
     5401 gagcgcatcc agttccttca agccatcaag cgaaaatgcc cgccgtgctg ccagcgattt
     5461 atcggcaatt tcgccgccca tgcgctcgac gatatcggag gcctgttcaa ggttgagcga
     5521 catttcgatg atctccgccc agcggcgcga ctcttcctcc gccaactctt cttttggcat
     5581 ccgcgccaga tacagcttaa tggcggtgta gagaacgttg atatcatctg ccagcttacg
     5641 cagctctttc tcctgccgtg gctcgccgtg catcacttta tttagccctt ccatcatctg
     5701 ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag caagcgtggg
     5761 cgtatccagc gcgctgacat ccagatgttt gggccgtagc tgggtatcca gttccggttc
     5821 atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca gcatgaccag
     5881 gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt ttggcaatga
     5941 caacttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca ggctacccac
     6001 cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg cggcactgtt
     6061 gttgagcatc gccagcagac cggaaccgag gttagcacca atcaccagac agagcgccac
     6121 ggggaaggag ataatgcctg cggcggtcag agtcgcggtc agcagtacag cagcaaggct
     6181 ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa tatcgccggt
     6241 cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta cggcctgcac
     6301 aatcaactcc agcgccagca aaatcagccc aagaccaata ccgacgcggc ccagttgccc
     6361 ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa gtaacggtga
     6421 cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga catctgcacc
     6481 cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacaa acgaggtcac
     6541 cagcatggtg gtggcattac tgctctgtac cagtgcggta acgccgatcc ccgcgcaaaa
     6601 ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg cgccgaagac
     6661 gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga cggcagaaag
     6721 caggtgaagc agcgttaaca cgagttcagg ttctccttat cgttatatgt tcctgaagca
     6781 attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc cttttgacct
     6841 tcgtttttcc ggctaagatt attccagcct gattccagta taagtgtttc atattaataa
     6901 agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca gatttgtgaa
     6961 tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacttcgg taacgctcat
     7021 acctttacgg cgggcataat cttcaacctg atcgcgctga atttgtgcta cagcgtagta
     7081 cttgctgtcc gggtggctga agtaccaacc cgaaaccgat gcaccgggcc acatggcgaa
     7141 agattctgtg agtttcatgc cagtgtgttt ttccacttcc agcagctccc agatggtggc
     7201 tttttccgta tgttccgggc aggccggata gcccggtgcc ggacggatgc cctggtagtt
     7261 ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc agtagacttt
     7321 acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg caagcgcttt
     7381 caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca gtgcgtcctc
     7441 ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag aaagcttcgg
     7501 cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct gttgacgcag
     7561 atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt caatgtcatc
     7621 gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg ttttctcggc
     7681 gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct caacgcccac
     7741 cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca taaagaacgg
     7801 tgtccagtcg atgtaattac gcagagtttc gatgctggct tcaacttcct gcacgccgag
     7861 acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa gcgaaatcgt tatcgcgcgc
     7921 cgcttccagc gtgaccggtg gtgtgcgcgg tttcttacgc ccgtgctgaa tacgtacggt
     7981 ttcgtactcc ttgcgggtac gagcgacaaa atcatcacgc tgagtatcgg aaagcagcgc
     8041 cgccaccaca ccaacggtgc gcgatgcgtt ctgtacatac accgtcgggc cgctgtagtt
     8101 ctgctcaatt ttcaccgccg tgtgcgcttt tgaggtcgtc gcgccgccaa tcaatagcgg
     8161 gatggtgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt ccagcgacgg
     8221 cgtgataagc cccgaaagtc caatcagatc agcattcact tctttagcgg tacggagaat
     8281 tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac attgcagcac
     8341 cacgccaacg atatttttac cgatgtcgtg gacatcgcct ttcacggtgg cgatcaccat
     8401 cttgccgttg gttttaccct gctctttgct ggcttcaata aacggttcga gatacgccac
     8461 tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt tcccttcgcc
     8521 aaacagatcg ccgaccacgt tcataccgtc catcagcggg ccttcaatca cttcaatcgg
     8581 gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg ataaactcgg taatgccttt
     8641 aaccagcgag tattccagac gtttattcac ttcccacgag cgccactccg cctgctgggc
     8701 gttagcggtg tcgtcggttt tgctgccgcg atatttctcg gcaagctcca gtaaacgctc
     8761 agtgccatcg tcgcgacggt taagaatcac atcttccacc gcgtcgcgca gttcagcggg
     8821 taggtcgtcg taaatcgcca gttgcccggc gttgacgatc cccatatcca tgccattgcg
     8881 aatagcgtag tagaggaaca ccgcgtgaat ggcttcgcgc accggatcgt tgccacggaa
     8941 tgagaaagaa acgttagata caccgccgga aatcagcgcg tgcggcagtt cgcgtttgat
     9001 atcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa tgccggttgc
     9061 gaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa cctcttcggt
     9121 gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgggtat cggcctgtcc
     9181 ctgttcgtca aaggccatta ccaccactgc cgcaccgtaa cgacgcaaga gcttcgcgtg
     9241 atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc ctttgccctg
     9301 gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca taatcggcac
     9361 gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg ccgcttcggc
     9421 gtcgagcatc ccttcatcca tgttgatatc gataatctgc gcaccgtttt ccacctgctg
     9481 gcgggccaca tccagtgcct cgctgtattt ctcttctttg atcaggcgct tgaacttagc
     9541 ggaaccggtg acgttggtgc gttcacccac gttcacaaac aggctatctt cgccaatgtt
     9601 cagcggctcc aggccggaca aacggcaggc tacggggatt tccggcagtt tgcgcggcgc
     9661 taatccttct actgcacgac tcatcgctgc aatatgttgt ggcgtggtgc cacagcagcc
     9721 gccgacgata ttgagaaaac ccgcttgcgc ccattcacgt atctgttttg ccatcgtgtc
     9781 ggcgtcgaga tcgtactcac caaaggcgtt gggtagcccg gcgttcgggt gcgcggtgac
     9841 gtagcattcc gcaatccgtg acagctcctg cacgtactgg cgcagttcat cgggccccag
     9901 cgcacagttc aggccaaagg tcagagcttc ggcgtggcgc aatgagttgt aaaatgcttc
     9961 ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg agatcataat
    10021 cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaaaaccg ccgctttggc
    10081 gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcgccacctt ccaccagcgc
    10141 tttggtggac tctcgataag ccgccaccag cccgtcaaaa gtgatattac gaaatgccgg
    10201 atcatttacg tccggagaaa tagacgccgt gcggttggtc gggccgagaa caccggcaac
    10261 gtagcgcggt ttctccggcg tgcgcgcggt ccactcatca gcacaagctc gcgccagttt
    10321 cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg ccatcgcaat
    10381 ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa agtaggcgtt
    10441 gtggatagcg gcgatcactt ccggtttgct gagcaccagc aggtcgttgt tgcctttgag
    10501 atcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca gtcgatagct
    10561 ctggatcatg gtgcccatac cgccgtccag caccagaata cgttgattta actgcgcacg
    10621 cagttgttcc actttgctgc tcacacttgc tcccgacacg ctcaacccag atttaataaa
    10681 aattcaacaa accatactgg cataaacgca tctgtggtaa aagcgaccac tacgcaacat
    10741 gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt agctaacaat
    10801 aaaaatgaaa atgatttcca cgatacagaa aaaggaggct gtcatggtcg cacccattcc
    10861 cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac aggttcagtc
    10921 tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca gtgtggcact
    10981 cacagagctg gcgcaacaag ccgggttacc caattccacg acccaccgcc tgctaaccac
    11041 gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg ggacattggg caatcggcgc
    11101 acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag cgattgttca
    11161 ccctatcctg cgcaatttaa tggaagagtc tggcgaaacg gtcaatatgg cggtgcttga
    11221 tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacgcatc tgatgcgaat
    11281 gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta aagccttttt
    11341 agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt tacatgccta
    11401 tacccacgca acgctggtgt ctcctgtaca tttaaaagaa gatctcgccc aaacgcgcaa
    11461 acggggttat tcatttgacg atgaggaaca tgcactgggg ctacgttgcc ttgcagcgtg
    11521 tattttcgat gagcaccgcg aaccgtttgc cgcaatttca atttccggac cgatttcacg
    11581 cattaccgat gacagcgtga ccgagtttgg cgcgatggtg attaaagcgg cgaaggaagt
    11641 gacgctggcg tacggtggaa tgcgctgact ttttctggcg ggcagaggca atattctgcc
    11701 catcatacct gagtggcaat agaataaggg tgtctgttaa tcgcattgac gccaaaataa
    11761 cttaatgtca tacacttcac acacttttat taactcagca ttatttttaa acatcaaacc
    11821 acttaatcat taacaaaatc gcaagatctc attccaccga aacttttacg gctgcattat
    11881 gagattgagc gatt
//
LOCUS       DAZGCS010000134        11877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGCS010000134 DAZGCS010000000
VERSION     DAZGCS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.136"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..812
                     /gene="csgD"
                     /locus_tag="ACHK01_004228"
     CDS             162..812
                     /gene="csgD"
                     /locus_tag="ACHK01_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO8099075.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPENYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            817..1206
                     /gene="csgE"
                     /locus_tag="ACHK01_004229"
     CDS             817..1206
                     /gene="csgE"
                     /locus_tag="ACHK01_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO8099076.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1231..1647
                     /gene="csgF"
                     /locus_tag="ACHK01_004230"
     CDS             1231..1647
                     /gene="csgF"
                     /locus_tag="ACHK01_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO8099077.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1674..2507
                     /gene="csgG"
                     /locus_tag="ACHK01_004231"
     CDS             1674..2507
                     /gene="csgG"
                     /locus_tag="ACHK01_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO8099078.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2571..3062)
                     /gene="ycdZ"
                     /locus_tag="ACHK01_004232"
     CDS             complement(2571..3062)
                     /gene="ycdZ"
                     /locus_tag="ACHK01_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8099079.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3164..3718)
                     /gene="ycdY"
                     /locus_tag="ACHK01_004233"
     CDS             complement(3164..3718)
                     /gene="ycdY"
                     /locus_tag="ACHK01_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO8099080.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3742..4479)
                     /gene="ycdX"
                     /locus_tag="ACHK01_004234"
     CDS             complement(3742..4479)
                     /gene="ycdX"
                     /locus_tag="ACHK01_004234"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO8099081.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4534..5472)
                     /gene="ghrA"
                     /locus_tag="ACHK01_004235"
     CDS             complement(4534..5472)
                     /gene="ghrA"
                     /locus_tag="ACHK01_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO8099082.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5706..5793
                     /locus_tag="ACHK01_004236"
     tRNA            5706..5793
                     /locus_tag="ACHK01_004236"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5740..5742,aa:Ser,seq:gga)
     gene            complement(5943..6785)
                     /locus_tag="ACHK01_004237"
     CDS             complement(5943..6785)
                     /locus_tag="ACHK01_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8099083.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6870..7067)
                     /locus_tag="ACHK01_004238"
     CDS             complement(6870..7067)
                     /locus_tag="ACHK01_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8099084.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7079..7570)
                     /locus_tag="ACHK01_004239"
     CDS             complement(7079..7570)
                     /locus_tag="ACHK01_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8099085.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7567..7941)
                     /locus_tag="ACHK01_004240"
     CDS             complement(7567..7941)
                     /locus_tag="ACHK01_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8099086.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7988..8362)
                     /locus_tag="ACHK01_004241"
     CDS             complement(7988..8362)
                     /locus_tag="ACHK01_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8099087.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8525..8746)
                     /locus_tag="ACHK01_004242"
     CDS             complement(8525..8746)
                     /locus_tag="ACHK01_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8099088.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8809..9285)
                     /locus_tag="ACHK01_004243"
     CDS             complement(8809..9285)
                     /locus_tag="ACHK01_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO8099089.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9301..9786)
                     /locus_tag="ACHK01_004244"
     CDS             complement(9301..9786)
                     /locus_tag="ACHK01_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8099090.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9877..10695)
                     /locus_tag="ACHK01_004245"
     CDS             complement(9877..10695)
                     /locus_tag="ACHK01_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8099091.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11035..>11877)
                     /locus_tag="ACHK01_004246"
     CDS             complement(11035..>11877)
                     /locus_tag="ACHK01_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO8099092.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT         2991 a         2992 c         2948 g         2946 t
ORIGIN      
        1 gctagattga aatcagatgt aatccattag ttttatattt tacccattta gggctgattt
       61 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
      121 gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat catgtttaat gaagtccata
      181 gtattcatgg tcatacatta ttgttgatca ctaaaccgtc tttgcaagcg acagctctct
      241 tgcagcacct taaacaatcg ctggcaatta caggaaaatt acataatatt caacgttctc
      301 tggacgatat atcttcaggc tctattattc ttctggatat gatggaagca gataaaaagc
      361 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      421 taaatacgcc tgaaaattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      481 ttttttatgc catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgcg
      541 gcgaatgcta ctttacgcaa aagcttgcca gttacctgat tacacattct ggtaactatc
      601 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc
      661 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      721 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      781 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      841 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      901 ggattgttaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      961 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
     1021 tggggaagct ggatcactat aacggtcaat caggacgtta ttttccagac ttttttattt
     1081 ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
     1141 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg gcgatttggc gcatgatgaa
     1201 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
     1261 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1321 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1381 tataaagatc cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac
     1441 tttactcagg ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt
     1501 aaaccgggcc gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa
     1561 ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt
     1621 ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc
     1681 gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag
     1741 aagccgccag accgacatta atgcctcgag ctcagagcta caaagatttg acccatctgc
     1801 cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat
     1861 ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc
     1921 tggtcacggc attgaaagat tctcgctggt ttataccgct ggagcgccag ggcttacaaa
     1981 acctgcttaa cgagcgcaag attattcgtg cggcacaaga aaacggcacg gttgccatta
     2041 ataaccgaat cccgctgcaa tctttaacgg cggcaaatat tatggttgaa ggttcgatta
     2101 tcggttatga aagcaacgtt aaatcgggcg gtgttggggc aagatatttt gggatcggtg
     2161 cagacacgca ataccagctc gatcagattg ccgtgaacct gcgcgtcgtc aatgtgagta
     2221 ccggtgagat cctctcctcg gtgaacacca gtaagacgat actttcctat gaagttcagg
     2281 ccggggtttt ccgctttatt gactaccagc gcttgctgga aggggaagtg ggttacacct
     2341 caaacgaacc cgttatgctg tgtctgatgt cggctatcga aacaggggtc attttcctga
     2401 ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa caaagcagaa cggcagaacg
     2461 acattctggt gaaataccgc catatgtcgg ttccaccgga atcctgacag ataaaaaagc
     2521 cggaattatc atccggcatt ttactgttta agcgaaagcg tgagtgcccc tcacgcttta
     2581 tttttgattt cctgctcacg gtgagccgtc tttgcactac gcgccgccag ccacaggccg
     2641 ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca gcgaaggtaa caccagtttc
     2701 cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa
     2761 agcagcagct gtttggcctg aatacacatc agaaacgcga caataccggt tatgacataa
     2821 ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa taatgaccat cgcccacacc
     2881 acgccactaa gcagggttgc agcggagatc gccagccctt tcagcccacc ttgcgggcag
     2941 gcaaagtagg ccgtacagcc caggaagcct gcccagctta gtaagcccag ggaaacagcc
     3001 acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag aagtatgttc
     3061 atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac attttatgca
     3121 acaacaaatt atctgttgca gttatgagtg acgatgatca catttactct tcagaatctt
     3181 cttccagctc gtcccacatt gcactaatgg catcacgggt tagcggtgcc atggtgcgcc
     3241 agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa cgcaccacac cagggtaaca
     3301 gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac tgatcttcca
     3361 gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc tggcgtatcc gccagtggca
     3421 tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt tgcgtcctca acccatgcgc
     3481 tacgatatgg cggcacggca cattcatcgc cgataaacaa cgcattgtaa tcggcagaga
     3541 cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa ctcatcctgc tccagtggcc
     3601 agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt aaaggatctt
     3661 gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac tcgttcatta
     3721 atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg
     3781 agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct ctggcggaaa
     3841 atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa acgcggtgtg
     3901 agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg ctaccgcacg
     3961 acagttgtct tcactgccct tacgtgagtg taaaaatgaa gaattattga tttccagcgc
     4021 cacctgatgt ttcgcggctg cttcagcaac ggctttcaca tctatttcat atttgggatt
     4081 tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca tcgcttgtgt
     4141 attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga aaaccggcaa taatcagatc
     4201 cagcgagtca aacattttac cgctgcaatc aatttcacca tcaacatttt taatgttagc
     4261 ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac gcatgttaat
     4321 gaagtgccag tgatgcggag catcttccat atccgggcca tgatcggtga tcgcaaaaag
     4381 tttaatgccc ttttgtttgg cctgggcaat gtaatcactc aatgtgctat atgcatgtgt
     4441 gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct gtattcaatt
     4501 cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg
     4561 cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt actccacagc
     4621 ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca
     4681 gagcggactt tcaggcggta agggttcacg attaaaaaca tccaacattg cgcctttaac
     4741 tttgccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac cacgcgccag
     4801 gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa taatgccgac
     4861 ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca gaaatgcaga
     4921 cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg ttcgactcca
     4981 gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc ccagtacgcc
     5041 tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg gttgccaatg
     5101 cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca gcacctgact
     5161 gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa aaagtggaac
     5221 agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat caacaccggc
     5281 cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag gaggatgcca
     5341 gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga ctcttgcctg
     5401 aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg ggtgataaaa
     5461 gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta
     5521 tgatctacaa aggccaccag cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc
     5581 gcttactaaa accacacatt gattgaagtt tgaataaacg cgcgattttt tcaaaaagtt
     5641 tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga caggaacgac
     5701 aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt atacggcaac
     5761 gtatcggggg ttcgaatccc cccctcaccg ccagattata agcgcatgtt attgtttaac
     5821 atgcgctttt ttattttccc ttccagacag tatgccatcg ggtatgccat ctgaaaccgg
     5881 tttatatgcc ccgcgatcat atcttatcgg acaatttccg atctttgagc atactggcaa
     5941 agctatactg ccagcatatc aggatagtgt tttgcgataa tatcattcag tctgtcaacc
     6001 agttctggtt ttctgaacgt gatatgcgcg gagcccttct taaagtatct gatgctgaac
     6061 atctcatctt catagctctc tttaccctga acagactgaa tgtgatcatc cagacgaagg
     6121 gtgatgtttc cccggttgtc ggggattggt ttaccgctga acaggtgcaa cattctttcc
     6181 agatcggcga gtcggtcagc ctgttgtccg gtgataagat gaaatcccca ccggtcccat
     6241 ctcaccagat tgttgacgat aattttactg ccaaatttac aggggcaatt ggttttgtaa
     6301 ttccagttca gccctctgaa gacgttgatc acccctcgct caaacacctc atccttattc
     6361 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatcg
     6421 tactccagtg accggtacca tgtatcacgg gcctgcgtat ccattaatga cagcatcccg
     6481 gaacgttgca tcaggtcacg ccagatttcg cgatcaagat tccgggtaat ggctttcatt
     6541 gctgtttcgg gtttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag
     6601 tccggggcag ttttacctcc gatgctttcc gtcagcaccg agatttcagc cagttgtcgg
     6661 ataagtgtgt cgatttgcaa cagtgtactc tttcgtacag ccagaatgtg gcgaatgctg
     6721 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgggagat tgtgatatct
     6781 gacataatcg atattccttt ttgcagataa atgaaaacgc cagccctgtg caggctggcg
     6841 tcagaggaat gaagtctggt gagtgagctt caccggttct ggtgcagaag ctgcagatga
     6901 cgcagggtac ggacatcctc ccgggcctgt tcgatacagg gcaacagtgc tgctgaatcc
     6961 gttttatcct catcgttgtc aaagataatt cccgatccgc agtcgatatt gtcctgcagc
     7021 cacgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt gttcctggtt
     7081 accagtcgaa agtgggtaaa cccggcaggc attcagcgct ggcatccaga tgaatgagac
     7141 tgacgccata acgccggatg agtgtgacga ccagacgacg gaacgttttt gacagcccca
     7201 ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt acgtaacaga tatccgctac
     7261 ctgtaaaatg aatccattct gattcaccga agtcactggt ctggtgtgac agcgagtaca
     7321 accaggcgtt gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg gtaagggtag
     7381 caaatggtgg cgtgcattct gctgaggtct ggtcagattc atcaacaaca cgaagggagt
     7441 agccgttatc gcagaccatg ttaatcagtt cagcgagctc aataccatcg acgtcaacga
     7501 caatgcgccc catgttcagt gcctgtacgt taacgctgtc ggcttccagt gtcagggcca
     7561 gtttcatcat ttcgcctccg gatacttacc caaggtaatg ttatttaccg ttctgtaatt
     7621 gtcgcgggtc atcaggccgg tcgcccggcg agcccggagg atatcaatac tgtttattaa
     7681 ctgagagcgg ggacaggtgc tgaatcccgg ctggtcggta cgcaccagcg cgtatttttc
     7741 cacgagaaaa ttaactgcat cacacagtga aatgcctgcc tcaatatgct gctcaatcac
     7801 acgttcatcg gcaaacggtg tgtcattcag tgtgaggcca taatgctggt ccagcagtcg
     7861 gatgagcagt gtctgccaga tggtgacggg agacgggcag cgagatacct cccgtacatg
     7921 tgtgtcggat aatgttttca tcttaagttc tctgtaactg gtagtgctaa aggtgagagc
     7981 ttcggggtta tgaggtggtt gcgagtgctg gtgtcggata aacagccaga taaacgtaac
     8041 cacaggagcc gagggtgtcg gcttcgcagg tcagcccttt tgcattcagc gtgacggtat
     8101 gctgatatcg gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca
     8161 gcggaaaggc ctggtccaga tggtatgcat ctgcgtcgct gaagcggcct ctgataccgg
     8221 cgcggtcggc aaggtaatgc aggcggttac cctcttgcac cagacgagcg ccaaaacagg
     8281 gcctcactgt gcagggaagc ccccaccagg ggcggtcgtg attgtcatcg ggatgagtta
     8341 tcccggagag tttgtctgac acgataaaat cctctctgaa aagtggttaa gaatggttta
     8401 acgatgacag gtcagccggc gcgctgaccg gtttcggtga agatgtatgc attgatgaga
     8461 atggcctcat ccacagcggc gtctgaggtc atctggtcgt actcgtttcc cgggtgacgg
     8521 aacatcagtt cagggtgaca ctcatcagcc ggacatacgg gccaaaactg tccttacggc
     8581 gttctgcaaa cacggcaaga acgccgggaa tatcctgcac ttcacgacca gtatacgctt
     8641 cagcactgcc gtgccagcga tacttaccgg tacagaacgg aaaaagacgg gatgtcggat
     8701 gttgttggtg aatacgcatg gcttcaccac gggtgataat tttcataatg ggatacctct
     8761 gaagacagaa gataaaagtg aaaacaggtg tgatgtggtt gtgaagggtt aaagcagacc
     8821 atgttcggca aaggagaaaa cctggttgcc accgactatc agatggtccg gcacccggat
     8881 atccaccagg cccagtgcct gtaccagacg ttcggtgata agccggtctg ccttactggg
     8941 tgtgacttca ccggacgggt gattgtgcgc cagcaccacg gcagcggcat tgtggtacag
     9001 ggcgcgttta atcacttccc ggggatgaac ttccgtacgg ttgatggtgc cggtgaagag
     9061 ggtttcaccg gcaatcagct gattctggtt gttcagatac agcacccgga actcttcccg
     9121 ctccagtccc gccatgttca gaatcagcca ttcccgtgcc gcacgggtgg aggtgaaggc
     9181 cacgccgggt tcatgaagat ggcggtccag ggtttgcagg gcccgcagaa tgagactgcg
     9241 ctcgccgggc gtcatctctc cgggcagaaa ggaaagctgc tgcattgtcc ctgcctccat
     9301 tcagtcaatg atgcgcataa tggcgctgca ttccggatgc tgcagggcgt aatcccgcag
     9361 ccggtaatag tggaccgtca tggcataaca ctccgtacga caggcatgat gactgtacgt
     9421 catcagacag gcagcaatac cggcggcttc cgggctcatt tcagcgcggt tgccgttcat
     9481 ggcattgaac agtacccatg tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc
     9541 cccgccgttg ttcagggtgt acagattcca gataccaccg cagtagtctt cgcaaagacg
     9601 gtccatccag ccgaagacac ggggctccag ggtcacccac tgtggaatga ggccaaaatg
     9661 ctgcggccag aagctgatgc gctgttcatc agggacggga gtggcaacca gctgaggtgg
     9721 gttattccct gatgcagtgg ttacggaaac agaaggtgtg gtggaattat gcgaaacggt
     9781 tgtcatgagg ttattcctta taaaaagtaa atgaatggaa gaagccccgg ggcaggaaca
     9841 gatgtgagtc aggactgcac tttcagtgaa acggcatcag cgcatgctct ccagcagcgt
     9901 ttctgccatc acccacaatg cgcgattgag cttaatgtcg gtatcaatgc tgtgaatggc
     9961 ccgggtatgg atacgttttc cttttgcact gcgaccggaa atcccgcctt tcagcatatt
    10021 ctcctggatg gtctgataag cactccacag gtccttaccg taatcctccc ggcgtcgtgg
    10081 tgtcagaatg tcggcggtgg tgacgggctg atgttcgtca ccataacggt aagtcagtgc
    10141 cgcctgtgcc agcgcctggc gtgccggtgg cggcagaatc agcgactgca tggcatcacg
    10201 cttctcctct atccggtcaa aaacgcccac cacctcgtaa gccccttcaa tgactctgtc
    10261 caccacattt ccccggtgtg gaacacgcaa ttctcccaga gactgaccgc agaccagtcc
    10321 attggtacaa atggcacgaa aatatccggg taacatctgg tagctggatg aaccgtcatg
    10381 ggagttgagc agaataattt cagggacatg ctgaccggtt atctgtccgg cccgccgcag
    10441 acgcagcatg tgttttgtgt attcccggcg gctctggtcg cgcacgcggg tctggcaggc
    10501 aaagaacggc tgaaagcctt cctgctgcag attttccagg acggtgatag tgggaatgta
    10561 cgcatagtgt tcactgcgag aggtatgtcg gttttcccca aaaatactcg gtacatggcg
    10621 catcagttct tcgtgtgtca gcggacgatc acgacgtatc tggttcgcat aaccaaaacg
    10681 actggctaat ctcatgattt gctccttatg aatgattaag cgatataaat gaaaaagccg
    10741 cgtctcccgg agaagacgcg gcctgatggg tgaaatgaat gaggtttatt gtctgagaag
    10801 cccttcactg gcgaaccacg tattaagctg tattccggca tcgccagtgc aactgacctt
    10861 cagcattgcg gataacccgc cggaaatatg ttcactggtt gtttttgtat cttcccccaa
    10921 aattaaataa attcatctta accatcaccc agataatata tatctgaaaa tattttcaat
    10981 aacaccagac aggaaaaaca ccaccacagg cggtggcgtt tctttggggc gtctttattt
    11041 aatccgggcc atcgcgtttc tcagtgccaa atctgcctct tccgttatat ccaggctttt
    11101 ctgcaatacc gcctctgacc gggtcagtgc ctgacgtgct gccagcggtt tccggatatg
    11161 ctccatcgcc agcatcgtgt cttcatagcc ctgctgacgc caggcgtcgg tatgtgccga
    11221 tgtaaaactg agcagaccgc cgctgttgcc accatcacct gcatatttca gccttttccg
    11281 gggacggatc tcaaggattg tggtgtccgg aaaagcctgg cgatcccata aagaaccgtc
    11341 actcagatgc gtcacaatca ccatattgca tccggcatcg accagaggtg tcacaggggt
    11401 atttccctgc atatttcgcc atcctcccat accaccatca ccgaacattg tcccctgaac
    11461 ctcacgggga cggaacagca ggggcagcgc ggctgacgca agcagagcct ctttctgctg
    11521 tccgcggggc aggctctgga tatgctgaaa aacggcgttt ttcgtggttc cgacacccag
    11581 ttcagcacga atgcagtcaa taatatcctg catgccccct tctgtggggt acagcgacac
    11641 atacagcggt agcccgtctg ccagagcatc agtatcaaga taatgatcca tcagcgctgt
    11701 caggggctca tctgacaaca acggctgagc catcagattg tcaaacccgt tgattgtcga
    11761 tatatggcga agaagcgtcg taagcagtgc tcctgcacgt ccggggatct ggcagaggtt
    11821 cattgcctgg aacaattttt tcagcaatga aaagtaaacc aatctgttta ccgacag
//
LOCUS       DAZGCS010000135        11070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGCS010000135 DAZGCS010000000
VERSION     DAZGCS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.137"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..1246
                     /gene="yghO"
                     /locus_tag="ACHK01_004247"
     CDS             104..1246
                     /gene="yghO"
                     /locus_tag="ACHK01_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO8099093.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGVEPYKRYRLYEKQI"
     gene            complement(1253..2017)
                     /gene="glcC"
                     /locus_tag="ACHK01_004248"
     CDS             complement(1253..2017)
                     /gene="glcC"
                     /locus_tag="ACHK01_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO8099094.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTNLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2268..3767
                     /gene="glcD"
                     /locus_tag="ACHK01_004249"
     CDS             2268..3767
                     /gene="glcD"
                     /locus_tag="ACHK01_004249"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO8099095.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3767..4819
                     /gene="glcE"
                     /locus_tag="ACHK01_004250"
     CDS             3767..4819
                     /gene="glcE"
                     /locus_tag="ACHK01_004250"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO8099096.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEL
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4830..6053
                     /gene="glcF"
                     /locus_tag="ACHK01_004251"
     CDS             4830..6053
                     /gene="glcF"
                     /locus_tag="ACHK01_004251"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO8099097.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6058..6462
                     /locus_tag="ACHK01_004252"
     CDS             6058..6462
                     /locus_tag="ACHK01_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO8099098.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6484..8655
                     /gene="glcB"
                     /locus_tag="ACHK01_004253"
     CDS             6484..8655
                     /gene="glcB"
                     /locus_tag="ACHK01_004253"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO8099099.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMDKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9010..10692
                     /gene="glcA"
                     /locus_tag="ACHK01_004254"
     CDS             9010..10692
                     /gene="glcA"
                     /locus_tag="ACHK01_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO8099100.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2587 a         2759 c         3077 g         2647 t
ORIGIN      
        1 actatttatt ctttgtttgt attcattggg ttgattatta aggtgtcgtt cgttgctgtt
       61 gcaacctgtt tttcaccgtt aattatttgt tatccacaga gatatggaat gcgatctgct
      121 gatgattaaa attgaaaaag taattaataa aaatgacctt aaggcattta tcgcttttcc
      181 gtcatcactt tatcccgacg atccaaactg gataccccct ttattcatag aacgcaacga
      241 gcatttgtct gcgaaaaatc cggggacgga ccatatcatc tggcaggcgt gggtggcaaa
      301 aaaagcgggg cagatagttg ggcgtattac cgcgcaaata gataccttgc accgtgagcg
      361 ttacggtgaa gataccggtc atttcggcat gattgacgcc attgatgatc cgcaggtttt
      421 tgccgcgttg tttggcgcag cggaagcgtg gttgaagtca caaggtgcaa gtaagatcag
      481 cggtcctttc agcctgaata tcaatcagga aagcggatta ctgattgaag gttttgacac
      541 accaccctgt gcgatgatgc cacacggcaa accgtggtat gccgcgcata ttgaacaact
      601 gggttatcac aaaggcattg atttactggc gtggtggatg cagcgaaccg atctcacttt
      661 ctctccggcg ctaaaaaaac tgatggatca ggtgcgcaaa aaggtgacca ttcgctgcat
      721 caatcgtcag cggtttgccg aagaaatgca gatcctgcgt gagattttca actctggctg
      781 gcagcacaac tggggatttg tgccgtttac cgaacatgaa ttcgcgacca tgggcgatca
      841 acttaaatat ctggtgccgg acgatatgat ctatatcgct gagattgatt ctgcaccctg
      901 cgcgtttatt gtcggcttgc cgaacatcaa cgaggcgatt gccgatctga acggatcgct
      961 ctttcccttc ggctgggcaa aattgctgtg gcgtttgaaa gtcagcggtg tgcgaactgc
     1021 gcgagtaccg ctgatgggcg tgcgcgacga gtatcagttc agccgcatcg gcccggtgat
     1081 tgcgctgtta ttgattgaag ccttacgcga tccgtttgcc cgccggaaga tcgatgcgct
     1141 ggagatgtca tggatcctcg aaaccaatac aggcatgaat aacatgctgg aacgcattgg
     1201 ggtggagccg tacaagcgtt accgattgta tgaaaagcag atttaatacc agctaactca
     1261 ggttcatctc cagcggcacc gccgagcgaa tcaaatggtg gccttccagc tcgatatcgt
     1321 ggagattctt tttcacggtc cgcacatgat cgcgtgctgc gcgctgggcg acgtgcggca
     1381 gccgctgcaa caccgcgttg tagatcctcg catgctggcg atcgatctgc tttttttgct
     1441 gtggtcgatg gtagagatta tttacgctgg caaacactga attaaacatc agattggtca
     1501 atgattgcag cgtaaacacc agcacctgat tgtgagaagc ctgacaaatg gcgagatgga
     1561 aagcgtgatc caactgcgca tgttcgatca gcgaaatctc tttgttgttc tcactggcgg
     1621 cgagcatttt ttcataacag cgggttatca caacaaaatc agcctgcgtt cccagcgttg
     1681 ccgccagcct tgccgattcg ccctccagta atgcgcgaac gtcgagcaga tcgtacagcg
     1741 ttcgcggctg cgtactgaac agatggatca gcgggctggt gtcctgcacc cgattaagcc
     1801 gtgcgacacg agaatcgcga ccctgcgccg tttcaataat cccgcgcccg cgcagcacgg
     1861 tcagcccttc acgcagtgcg gagcgtgaga agccgagctt ttcacacagt cgacgttccg
     1921 agggaagtgg ctgaccgacc ttcagtacgc cgtcgataat taaccgttcg atactctctg
     1981 caaccacttc gcaaataggg cgacgttcat ctttcattcc cggacctcgt gcacaggtag
     2041 gaccaatttt tctgttctta actcgcaaaa cacgcacatc acgtaagtga ttatgttaca
     2101 tcaatttaac attgagttaa ccaaggcaag gtcacagagc tggaaaaaaa atggtctgac
     2161 cggtagctaa agagatagac gaaaacgaaa agcccgctta ataactgttc acagaagcag
     2221 cgcgcaaaaa tcagctgcca cacaacacaa caaagcgaag cctactcatg agcatcttgt
     2281 acgaagagcg tcttgatggc gctttacccg atgtcgaccg cacatcggta ctgatggcac
     2341 tgcgtgagca tgtccctgga cttgagatcc tgcataccga tgaggagatc attccttacg
     2401 agtgtgacgg gttgagcgcg tatcgcacgc gtccattact ggttgttctg cctaagcaaa
     2461 tggaacaggt gacagcgatt cttgctgtct gccatcgcct gcgtgtaccg gtggtgaccc
     2521 gtggtgcagg caccgggctt tctggtggcg cgctgccgct ggaaaaaggt gtgttgttgg
     2581 tgatggcgcg ctttaaagag atcctcgaca ttaaccccgt tggtcgccgc gcgcgcgtgc
     2641 agccaggcgt gcgtaacctg gcgatctccc aggccgttgc accgcataat ctctactacg
     2701 caccggaccc ttcctcacaa atcgcctgtt ccattggcgg caacgtggca gaaaatgccg
     2761 gcggcgtcca ctgcctgaaa tatggtctga ccgtacataa cctgctgaaa attgaagtgc
     2821 aaacgctgga cggcgaggcg ctgacgctgg gatcggacgc gctggattca cctggttttg
     2881 acctgctggc gctgttcacc ggatcggaag gtatgctcgg cgtgaccacc gaagtgacgg
     2941 taaaactgct gccgaagccg cccgtggcgc gggttctgtt agccagcttt gactcggtag
     3001 aaaaagccgg acttgcagtt ggtgacatca tcgccaatgg cattatcccc ggcgggctgg
     3061 agatgatgga taacctgtcg atccgcgcgg cggaagattt tattcatgcc ggttatcccg
     3121 tcgacgccga agcaattttg ttatgcgagc tggacggcgt ggagtctgac gtacaggaag
     3181 actgcgagcg ggttaacgac atcttgttga aagcgggcgc gactgacgtc cgtctggcac
     3241 aggacgaagc agagcgcgta cgtttctggg ccggtcgcaa aaatgcgttc ccggcggtag
     3301 gacgtatctc cccggattac tactgcatgg atggcaccat cccgcgtcgc gccctgcctg
     3361 gcgtactgga aggcattgcc cgtttatcgc agcaatatga tttacgtgtt gccaacgtct
     3421 ttcatgccgg agatggcaac atgcacccgt taatcctttt cgatgccaac gaacccggtg
     3481 aatttgcccg cgcggaagag ctgggtggga agatcctcga actctgcgtt gaagttggcg
     3541 gcagcatcag tggggaacat ggcatcgggc gagaaaaaat caatcaaatg tgcgcccagt
     3601 tcaacagcga tgaaatcacg accttccatg cggtcaaggc ggcgtttgac cccgatggtt
     3661 tgctgaaccc tgggaaaaac attcccacgc tacaccgctg tgctgaattt ggtgccatgc
     3721 atgtgcatca cggtcattta cctttccctg aactggagcg tttctgatgc tacgcgagtg
     3781 tgattacagc caggcgctgc tggagcaggt gaatcaggca attagcgata aaacgccgct
     3841 ggtgattcag ggcagcaata gcaaagcctt tttaggtcgc cctgtcaccg ggcaaacgct
     3901 ggatgtgcgt tgtcatcgcg gcattgttaa ttacgacccg accgagctgg tgataaccgc
     3961 gcgtgccgga acgccgctgg tggcgattga agcggcgctg gagagcgccg ggcaaatgct
     4021 cccctgcgag ccgccgcatt atggtgaaga agccacctgg ggcgggatgg tcgcctgcgg
     4081 gctggcgggg ccgcgtcgcc cgtggagcgg ttcggtccgc gattttgtcc tcggcacgcg
     4141 catcattacc ggcgctggaa aacatctgcg ttttggtggc gaagtgatga aaaacgttgc
     4201 cggatacgat ctctcacggt taatggccgg aagctacggt tgtcttggcg tgctcactga
     4261 aatctcgatg aaagtgttac cgcgaccgcg cgcctccctg agcctgcgtc gggaaatcag
     4321 cctgcaagaa gccatgaacg aaatcgccca gtggcaactc cagccgctac ccattagtgg
     4381 cttatgttac ttcgacaatg cgttgtggat ccgccttgag ggcggcgaag gatcggtaaa
     4441 agcggcgcgt gaactgctgg ggggcgaaga ggttgcgggt cagttctggc agcaattgcg
     4501 tgaacaacaa ctgccgttct tctcgttacc aggtacctta tggcgcattt cattacccag
     4561 cgatgcgccg atgatggatt tacccggcga gctactgatc gactggggcg gagcgttacg
     4621 ctggctgaaa tcgacagccg aggacaatca aatccatcgc atcgcccgca acgctggcgg
     4681 tcatgcgacc cgctttagtg ccggagatgg tggctttgcc ccgctatcgg ctcctttatt
     4741 ccgctatcac cagcagctta aacagcagct cgacccttgc ggcgtgttta accccggtcg
     4801 catgtacgcg gaactttgag gagcaggcta tgcaaaccca attaactgaa gagatgcggc
     4861 agaacgcgcg cgcgctggaa gccgacagca tcctgcgcgc ctgtgttcac tgcggatttt
     4921 gtaccgcaac ctgcccaacc tatcagcttc tgggcgatga actggacggg ccgcgcgggc
     4981 gcatctatct gattaaacag gtgctggaag gcaacgaagt cacgcttaaa acacaggagc
     5041 atctcgatcg ctgcctcact tgccgcaatt gtgaaaccac ctgtccttct ggtgtgcgct
     5101 atcacaattt gctggatatc gggcgtgata ttgtcgagca gaaagtaaaa cgcccactgc
     5161 cggagcgaat actgcgcgaa ggattgcgcc aggtagtgcc gcgtccggcg gtcttccgtg
     5221 cgctgacgca ggtagggctg gtgctgcgac cgtttttacc ggaacaggtc agagcaaaac
     5281 tgcctgctga aacggtgaaa gctaaaccgc gtccgccgct gcgccataag cgtcgggttt
     5341 taatgctgga aggctgcgcc cagcctacgc tttcgcccaa caccaacgcg gcaactgcgc
     5401 gagtgctgga tcgtctgggg atcagcgtca tgtcagctaa cgaagcaggc tgttgtggcg
     5461 cggtggacta tcatcttaat gcgcaggaga aagggctggc acgggcgcgc aataatattg
     5521 atgcctggtg gcccgcgatt gaagcaggtg ccgaggcaat tttgcaaacc gccagcggtt
     5581 gcggcgcgtt tgtcaaagag tacgggcaga tgctgaaaaa cgatgcgtta tatgccgata
     5641 aagcgcgtca agtcagtgaa ctggcggtcg atttagtcga gcttctgcgc gaggaaccgc
     5701 tggaaaaact ggcaattcgc ggcgataaaa aactggcctt ccactgcccg tgtaccctgc
     5761 aacatgcgca aaagctgaac ggcgaagtgg aaaaagtgtt gcttcgtctt ggatttacct
     5821 taacggacgt tcccgacagc catctgtgct gcggttcagc gggaacatat gcgttaacgc
     5881 atcccgatct ggcacgccag ctgcgggata acaaaatgaa tgcgctggaa agcggcaaac
     5941 cggaaatgat cgtcaccgcc aacattggtt gccagacgca tctggcgagc gccggtcgta
     6001 cctctgtgcg tcactggatt gaaattgtag aacaagccct tgaaaaggaa taaaaaaatg
     6061 aaaactaaag tcattcttag ccagcaaatg gcgagtgcaa ttattgccgc aggtcaggaa
     6121 gaggcgcaga aaaataactg gtctgtttcc attgctgttg ccgatgacgg cggtcatctg
     6181 ctggcgttaa gtcgcatgga cgattgcgcg ccgattgcgg cttacatctc ccaggagaaa
     6241 gcgcgtaccg ccgcgctggg gcgtcgtgaa actaagggct atgaagagat ggtgaacaac
     6301 ggacgtaccg cgttcgtgac tgcgccgtta ctgacgtcgc tggaaggtgg cgtaccggtt
     6361 gttgttgatg ggcaaattat tggtgccgtg ggcgtttctg gtttaaccgg agcacaggat
     6421 gcgcaggtcg cgaaagcggc agcagcggtg ttggcgaaat aagcgaaaac gaggagataa
     6481 acaatgagtc aaaccataac ccagggccgt ttacgcattg acgccaattt taaacgtttt
     6541 gtggatgaag aagttttacc gggaacaggg ctggacgctg cggcgttctg gcgcaatttt
     6601 gatgaaatcg ttcacgatct ggcaccagaa aatcgtcagt tgctggcaga gcgcgatcgc
     6661 attcaggcgg cgcttgatga gtggcatcgc agcaatccgg ggccggtaaa agataaagcg
     6721 gcctataaat ctttcctgcg tgaactgggc tacctggtgc cgcaaccgga gcgcgtgacg
     6781 gtggaaacca cgggcattga cagcgaaatc accagtcagg cggggccaca gctggtggtt
     6841 ccggcaatga acgcccgcta cgcgctgaac gcggcgaacg ctcgctgggg ctcactgtac
     6901 gatgcgttat acggcagcga catcatcccg caggaagggg cgatggtcag cggctacgat
     6961 ccgcaacgcg gtgagcaggt tatcgcctgg gttcggcgtt tcctcgatga atctctaccg
     7021 ctggaaaacg gcagctatca ggatgtggtg gcgtttaagg tggtcgataa acaattacgc
     7081 atccagttga aaaatggtaa agaaaccacg ttacgtaccc cggcgcagtt tgtcggttac
     7141 cgtggcgatg ccgctgcgcc gacctgcatt ttgctgaaaa ataacggcct gcatattgaa
     7201 ctgcaaattg atgccaacgg gcggattggc aaagacgatc cggcgcacat caacgatgtt
     7261 atcgtcgaag cggccatcag taccattctc gactgcgaag attcggtcgc ggcggttgat
     7321 gcggaagata aaatcctgct gtaccgcaat ctgctgggcc tgatgcaggg gactctgcaa
     7381 gagaaaatgg ataaaaacgg tcggcaaatc gtgcgtaaac tgaatgatga tcgtcagtac
     7441 accgccgccg atggctctga aatttctctg cacggacgct cgttgctgtt tatccgcaac
     7501 gtgggtcatt tgatgaccat tcctgtgatt tgggacagcg aaggcaatga aatcccggaa
     7561 ggcattcttg atggcgtcat gactggcgcg attgccctct atgatttaaa agtgcagaaa
     7621 aactcgcgca ctggcagcgt ctatattgtg aaaccgaaaa tgcacggtcc gcaggaagtg
     7681 gcgttcgcca acaaactgtt tacccgcatt gagacaatgc tcggtatggc accgaatacc
     7741 ctgaaaatgg gcattatgga tgaagaacgt cggacctcgc tgaacttgcg tagctgtatc
     7801 gctcaggcgc gtaaccgcgt ggcgttcatc aataccggtt tcctcgaccg taccggcgat
     7861 gaaatgcatt cggtgatgga agctggcccg atgctgcgta aaaaccagat gaaatcgacg
     7921 ccttggatca aagcctacga acgtaataac gtgctttccg gcctgttctg tgggctgcgc
     7981 ggtaaagcgc aaatcggtaa aggcatgtgg gcaatgccgg acctgatggc agacatgtac
     8041 agccagaagg gcgaccaact gcgtgccggg gcaaacacag cctgggttcc gtcgccaact
     8101 gccgctacgc tccatgcgat gcactaccac caaaccaacg tacagagcgt gcaagccaac
     8161 attgcccaga ccgagttcaa tgctgaattt gaaccgctgc tggacgatct gctgactatt
     8221 ccggtggctg aaaacgctaa ctggtcggtg gaagagatcc aacaagagct ggataacaac
     8281 gtgcagggga ttctggggta cgtggtgcgc tgggtggagc aggggattgg ttgttcaaaa
     8341 gtgccggata ttcacaatgt ggcgctgatg gaagaccgcg caacgctgcg tatctccagc
     8401 cagcatatcg ccaactggtt acgtcacggt attctgacca aagaacaggt gcaggcgtcg
     8461 ctggagaata tggcgaaagt ggttgatcag caaaacgctg acgatccggc ttatcgtccg
     8521 atggcgggga atttcgctaa ctcgtgtgct tttaaagctg ccagcgattt aatcttcctc
     8581 ggcgtgaaac agccaaacgg ctataccgaa ccgttattac acgcctggcg tttacgcgaa
     8641 aaagaaagtc attaatagcg tcaaaaaagc cccggcaagt tttattgtcg gggctatgat
     8701 agtaactgcg gtagtacatt tatcccggaa ataaccggag cgattatcag ggtttaacaa
     8761 tattaaatac ccgtagcgca ttactagcgg gcctaaaaca acaatatgga catcttaacg
     8821 atactctgcg cctgcgtgtg cggaacagaa acccttaccc gatatacttg ttatgccaga
     8881 ggtagcaatt gcgttagcgg cgtactgttg ctgtaaagta taacgtctat attttacagt
     8941 tcagtcctct tttgcttatt acaaccttaa gtatatcaag catataaaga taataagaga
     9001 ctgaacaata tggttacctg gacccaaatg tatatgccaa tgggaggact ggggctatcc
     9061 gctctggtcg ccctgatccc gataatattc ttcttcgttg cactcgcggt attacgtctg
     9121 aaaggacatg tcgctggagc aataaccctt atattatcta tcctgattgc aatattcgcc
     9181 tttaaaatgc cgattgatat ggcttttgct gctgcgggct atggctttat ttatggatta
     9241 tggcctattg cgtggattat tgtcgcggcg gtgttcctct ataaattaac cgttgccagc
     9301 ggtcagttcg atattatccg cagctcggtt atctccatca ccgacgatca gcgtttgcag
     9361 gtgttactga ttggtttctc ctttggtgcg ttgctggaag gagcggctgg ctttggtgcg
     9421 ccggtggcga ttaccggtgc gctgctggtg ggcctgggct tcaaaccgtt atacgcggcg
     9481 gggctgtgtc tgattgccaa cactgcgcca gtggcgtttg gtgcgttggg cgtgccgatt
     9541 ctggtcgccg gtcaggtaac gggaatcgat ccgttccaca ttggcgcaat ggcgggacgt
     9601 cagttaccgt tcctgtcggt tcttgtgccg ttctggctgg tagcaatgat ggacggctgg
     9661 aaaggggtga aagagacgtg gccagcggcg ctggttgctg ggggaagctt cgctgtcact
     9721 cagttcttta cctctaacta tattggtccg gaactgccgg atattacttc ggcgctggtg
     9781 agtatcgtct cactcgcttt attccttaaa gtctggcggc cgaaaaatac cgaaacggca
     9841 atcagcatgg ggcaatccgc aggtgcgatg gtggtaaata agccatcttc tggcggtccc
     9901 gtgccttcag aatatagtct ggggcaaatc attcgtgcgt ggtcaccgtt tttaatctta
     9961 acggtgctgg tcaccatctg gaccatgaag ccgtttaaag cgttatttgc tccgggcggc
    10021 gcgttttatt cactggtgat taatttccag atccctcatt tgcatcaaca agtgttgaaa
    10081 gcggcaccca ttgtcgccca accaacgcca atggatgcgg tgtttaaatt cgaccctctc
    10141 tcggctggcg gcaccgctat ttttattgcg gcgattatct ctatcttcat cctcggcgtg
    10201 ggcatcaaga aagggattgg cgtctttgcc gaaacgctaa ttagtctgaa gtggccgata
    10261 ttgtcgattg gcatggtgct ggcgttcgcc ttcgtcacca actattccgg catgtccacc
    10321 acgctggcgc tggtactggc aggtacaggc gtgatgttcc cgtttttctc accgttcctc
    10381 ggctggctgg gggtattcct taccggctcg gacacttctt caaacgccct gtttggttca
    10441 ctgcaatcga ctacggcgca gcaaatcaac gtctctgaca ctctgctggt ggcagcaaat
    10501 accagcggcg gcgtaactgg caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc
    10561 gcgacgggca tggtgggccg agaatctgaa ctattccgct acaccgtgaa gcacagtctg
    10621 atttttgcca gcgttatcgg cgttatcacg ttgttgcagg cgtatgtgtt tactggaatg
    10681 ttggtctcgt aaatcaataa tgccggatga tcgttaatat catccggctt catttacatt
    10741 attctgtctt attaattgaa aattatttct cgctttatta tccatgcggc aataatgttt
    10801 tgtgttcaga taacctgata aataattaag agcgtgagaa catctatttt tttatcacgc
    10861 aaaacaagtc tgtattaatt atgttcgctt gatgagcaaa caccgttcta tctgttgctt
    10921 ttgcaatctt aatcgttaaa actaccaacg aatttatata gttgcgttgt gaaatgttgt
    10981 taattttttg tttattgaaa tttcgtaaaa atagccagcc agacatcaaa taggggttat
    11041 tcttataaat ttcacctgtt ttctatcaat
//
LOCUS       DAZGCS010000136        10895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGCS010000136 DAZGCS010000000
VERSION     DAZGCS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.138"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..780
                     /gene="gadW"
                     /locus_tag="ACHK01_004255"
     CDS             52..780
                     /gene="gadW"
                     /locus_tag="ACHK01_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO8099101.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1143..4256)
                     /gene="mdtF"
                     /locus_tag="ACHK01_004256"
     CDS             complement(1143..4256)
                     /gene="mdtF"
                     /locus_tag="ACHK01_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO8099102.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4281..5390)
                     /gene="mdtE"
                     /locus_tag="ACHK01_004257"
     CDS             complement(4281..5390)
                     /gene="mdtE"
                     /locus_tag="ACHK01_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO8099103.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5498..5776
                     /locus_tag="ACHK01_004258"
     CDS             5498..5776
                     /locus_tag="ACHK01_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099104.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5777..6304)
                     /gene="gadE"
                     /locus_tag="ACHK01_004259"
     CDS             complement(5777..6304)
                     /gene="gadE"
                     /locus_tag="ACHK01_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO8099105.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7103..7675)
                     /gene="hdeD"
                     /locus_tag="ACHK01_004260"
     CDS             complement(7103..7675)
                     /gene="hdeD"
                     /locus_tag="ACHK01_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO8099106.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            7930..8262
                     /gene="hdeA"
                     /locus_tag="ACHK01_004261"
     CDS             7930..8262
                     /gene="hdeA"
                     /locus_tag="ACHK01_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO8099107.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8378..8704
                     /gene="hdeB"
                     /locus_tag="ACHK01_004262"
     CDS             8378..8704
                     /gene="hdeB"
                     /locus_tag="ACHK01_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO8099108.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8768..9415
                     /gene="yhiD"
                     /locus_tag="ACHK01_004263"
     CDS             8768..9415
                     /gene="yhiD"
                     /locus_tag="ACHK01_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO8099109.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9457..9987)
                     /gene="dctR"
                     /locus_tag="ACHK01_004264"
     CDS             complement(9457..9987)
                     /gene="dctR"
                     /locus_tag="ACHK01_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8099110.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10143..10709)
                     /gene="slp"
                     /locus_tag="ACHK01_004265"
     CDS             complement(10143..10709)
                     /gene="slp"
                     /locus_tag="ACHK01_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO8099111.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         2972 a         2486 c         2510 g         2927 t
ORIGIN      
        1 attagtatac tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat
       61 gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata
      121 tcgctgcata acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt
      181 tgttcaccgg atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa
      241 aatattcgtc agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac
      301 cagtcacccc ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta
      361 aattcaccga tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag
      421 aaagaactga tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc
      481 cttattagct ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat
      541 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
      601 ttactcgctt ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg
      661 catactattg cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga
      721 caatattatg gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga
      781 catattttgc atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc
      841 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
      901 attttcagga ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt
      961 acgggaatgt gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc
     1021 tactttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     1081 gaatggttag caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca
     1141 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     1201 aagtaaattg ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg
     1261 ttttgcgcgc cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     1321 gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct
     1381 atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg
     1441 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt
     1501 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     1561 accgagaacg gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc
     1621 agtgaaatgg catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt
     1681 ccggtccatg agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc
     1741 atagcgtcac cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg
     1801 ccgttgtagc gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa
     1861 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     1921 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     1981 tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc
     2041 acacccattg cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc
     2101 aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc
     2161 tcgtttcgtg cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata
     2221 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     2281 gctttattga tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt
     2341 tcctcaccga cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc
     2401 ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct
     2461 tttgtaagat aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca
     2521 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     2581 gtcggtgtac acaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     2641 tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt
     2701 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg
     2761 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc
     2821 agcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc
     2881 ataaaggcca tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt
     2941 tggatctgcc ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg
     3001 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtagccc tatcgccagc
     3061 accatcccga acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     3121 gtcccgagaa taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc
     3181 aaaaacagat acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact
     3241 tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt
     3301 gcggggaaat aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg
     3361 gcgtttgctc cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     3421 gccacggtgg aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact
     3481 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     3541 cgcgtctgca caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc
     3601 agttgaccac cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt
     3661 accagattgt atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     3721 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     3781 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct
     3841 accatcagaa tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca
     3901 ggtaatgaag gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga
     3961 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     4021 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     4081 tcttctaccg tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc
     4141 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     4201 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     4261 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     4321 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     4381 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     4441 atttcgcgta gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     4501 gcgttgtggg tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc
     4561 actaatgccg tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc
     4621 cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg
     4681 gtctggctgt agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
     4741 tgtttgattt gcccactggc gacctcttct ttcatgcgta agaaatcctg caccgactgc
     4801 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
     4861 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
     4921 gtgacattcg cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg
     4981 acattggctt ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg
     5041 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
     5101 gagagcgctt tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga
     5161 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
     5221 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg
     5281 ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc
     5341 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
     5401 aaaaacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
     5461 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
     5521 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
     5581 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     5641 gggttctact ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt
     5701 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     5761 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     5821 gtcttagttt cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     5881 tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt
     5941 tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag
     6001 taatctcaag aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag
     6061 cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct
     6121 ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct
     6181 tgaagggttt attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt
     6241 gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa
     6301 tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat
     6361 ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac
     6421 taatacgtct attaacacta atcaatactt cagatttatc catcacgcac tccatatatt
     6481 gatcctaagt aagattcctg gttgttatca atttgtaaat atcaaacaaa aagttaacgt
     6541 ttgcggatta ctgcccaaga ataagttatc aataaccagt ttgtgatctc tgaagaatat
     6601 tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg acttaagctg
     6661 gcaattggat tgccagcttt cttttaacgc attgaaatta cctgagtaaa taacgcattc
     6721 atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg
     6781 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
     6841 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac tacggaaaat
     6901 atcagccatg ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa attagatgaa
     6961 attcagtagg ttgaaatgat cactagcagg taattatttc aatgatagtg cgcaattgat
     7021 ctacaacact gcgtagcgga gagagtatta atcggatcat agtcacatca agtgactatg
     7081 atccgggtga caaccgggat aattattgct gcttaacgaa caaactggcg aagctgaaca
     7141 ggctggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttacagaca
     7201 ccatcggcgt tgcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
     7261 gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
     7321 ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctccggtg
     7381 cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
     7441 ccggccagaa attatgactg cggttgctga ataacccgac aataagcgca atacccgagc
     7501 agattaataa tgcacccact actgtgctta aaatatcgcc agagacgaac gggaaactga
     7561 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgct
     7621 ttttcagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac
     7681 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa
     7741 gaatgcagtc gatttaataa aaatttccta attgcagtat ctgatgcatc tgtaactcat
     7801 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
     7861 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
     7921 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagtt
     7981 gtgagcaatg cagcggatgc gcaaaaagca gctgataaca aaaaaccggt caactcctgg
     8041 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
     8101 gaagcgctga acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca
     8161 accgtaaccc cagctatcgt tcaggcttgt actcaggata aacaagccaa ctttaaagat
     8221 aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca
     8281 tcgtacttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc
     8341 tgttcatata taatctctat attgaatggg ttacaaaatg aatatttcat ctctccgtaa
     8401 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct
     8461 ggcagccaat gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
     8521 agcaatgacc ccggttgcat ggtggatgct gcatgaagaa acagtatata aaggtggcga
     8581 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgtaa
     8641 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
     8701 ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
     8761 tttttatatg acagcagaat ttattatacg tcttatactc gcggcaattg cctgtggcgc
     8821 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
     8881 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
     8941 tttagatcac gtcggactcg accccagccg tattgctgct caggtggtga cgggcgtcgg
     9001 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
     9061 agcggatatc tgggtgaccg ccgccatagg tatggttatt ggcagcggta tgtacgaact
     9121 gggtatttat ggttcagtga tgaccttgtt ggtgctggaa gtcttccatc aattaacctt
     9181 ccgcctgatg aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc
     9241 aatgctcgac tggtttaagc agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa
     9301 tgaagatcat gaagtggtcg ccattgatat ccagcttcat gccacaacgt cgatagaaga
     9361 cctgttgcga ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt
     9421 gtggcagcgt agccagactc accgtaagcc tgaaattcac accagataat caatatgctg
     9481 atggcgtacc agctcattga tccgcttaag attgaggatc atcatgatat tgtgtttatg
     9541 gcagtagaac gtttttagcg atattttcag tttctccaga atatcttccg ttgtcatttg
     9601 attcgcaatg cagctcagaa cctgatgttg cttgcgcgaa agtgacacag ccgcttcagg
     9661 cacagagcag taactgccat tgatgagtgc gacaaaatca atgatagtga tagtccgcgg
     9721 aacaaaggtc accggcactt tccccaggca gcgtttaata ttaaaggggg agaaaataat
     9781 cacatggaca ggttttaatc gttcaatttg agtgagaaaa ttagaatgaa atacattatt
     9841 cattaatgta tcaataatga cgaaggcatt ctgatgcaac attacgtcga tctcttcttc
     9901 gttctgtata tgaacgacat tacctttact cagaatgttt ttcatcgcgg tgaagaacat
     9961 cgtatccctg gtaattataa gaaacataac cgactcctgg tgcggacatt aactttataa
    10021 tcagtttgcc aaaggtaatt aacaggacag ggcttataat aataatagtt agcttactgt
    10081 atattaattt tatgtaatac atgatgcaga tgctgagaaa gcatctgcat ctttcggtgg
    10141 tgttatttga ccagctcagg tgttacctga ctcaccgcat tggtgtagta aggcgcaccc
    10201 cagcccggtt ccggccagaa tgcaccatag ccgtaatccc acaggttata ggtggtatta
    10261 accacttctc tcaaatgcca cacctggatg ccctgcatat tcacttccag gaagttatac
    10321 gggactttat tgataaagcc tggttgttca ccctgaatgg taccgaggat ggtaacaaag
    10381 tgattacgat agttcactgg atcaaggaag ccgctttgtc tggcgagcag tcggccctga
    10441 tagttggctt caatatcagg cttcgcatag ctatccaacg gtaatacagc gatttctaac
    10501 aacgtatccg ttttgccatt gataacgttg ataaccttcc caccaaagcg cgcttgttga
    10561 ccaacatata accccggctg gttatgaaca gcaacaaaac ttttttgaat atcaggttgg
    10621 ttattgcctt tgatattttg cggaattgaa ctacatgcgg caagcaaaaa tgaaaggctg
    10681 aggatgagtg caccttttgt catgttcatg ttactatcct tatcaacaaa tattaaaacc
    10741 ataatagata tttctttata gttttcatct gattctgagg tgatatgcct gatagtaacg
    10801 taaacctgtc tgatgtttat atcccttctg tttagttcaa ttccgttatt ttggggcgtg
    10861 gtatgagtat ccatcgccca ataatataac ccttt
//
LOCUS       DAZGCS010000137        10891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGCS010000137 DAZGCS010000000
VERSION     DAZGCS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.139"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..800
                     /gene="yghS"
                     /locus_tag="ACHK01_004266"
     CDS             87..800
                     /gene="yghS"
                     /locus_tag="ACHK01_004266"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8099112.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            832..1589
                     /locus_tag="ACHK01_004267"
                     /pseudo
     CDS             832..1589
                     /locus_tag="ACHK01_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1635..2984
                     /locus_tag="ACHK01_004268"
     CDS             1635..2984
                     /locus_tag="ACHK01_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO8099113.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2984..3808
                     /locus_tag="ACHK01_004269"
     CDS             2984..3808
                     /locus_tag="ACHK01_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099114.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLIRKQTKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3820..4380
                     /locus_tag="ACHK01_004270"
     CDS             3820..4380
                     /locus_tag="ACHK01_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8099115.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4411..5481
                     /gene="lptF"
                     /locus_tag="ACHK01_004271"
     CDS             4411..5481
                     /gene="lptF"
                     /locus_tag="ACHK01_004271"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8099116.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALYVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5478..6557
                     /gene="lptG"
                     /locus_tag="ACHK01_004272"
     CDS             5478..6557
                     /gene="lptG"
                     /locus_tag="ACHK01_004272"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8099117.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6593..7765)
                     /locus_tag="ACHK01_004273"
     CDS             complement(6593..7765)
                     /locus_tag="ACHK01_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8099118.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7765..8013)
                     /locus_tag="ACHK01_004274"
     CDS             complement(7765..8013)
                     /locus_tag="ACHK01_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8099119.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLPPWLENK"
     gene            complement(8045..8959)
                     /locus_tag="ACHK01_004275"
     CDS             complement(8045..8959)
                     /locus_tag="ACHK01_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8099120.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8956..10677)
                     /locus_tag="ACHK01_004276"
     CDS             complement(8956..10677)
                     /locus_tag="ACHK01_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO8099121.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
BASE COUNT         2635 a         2692 c         2844 g         2720 t
ORIGIN      
        1 attaaccatt gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca
       61 atacattatt tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca
      121 cgccagtgcg tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg
      181 caagcctggt aaatgaactg gcagcaagaa tgccaacaga acacatttat ctcgggcaat
      241 cgtccgggcg aattggcgaa tggatttcac agctccctgt tattggcgca ccttgtgggc
      301 gttatctgcg aagtaaagcg gcacatgtgc acgaaaaacc ctcaacaccg cctggcaata
      361 ttactgcgct ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt
      421 tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg ctacccgcag gttgaagtgc
      481 cgggttttcg ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa
      541 aaatgttaag gcagcgcgag ctgaagctgt accaatggat ggcatcttat ttgcccgtat
      601 tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac
      661 tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggcgcaaaga
      721 tccttgaact ggatgggcga caccccgccg atgaaattct gcaagcgtca ctacgcgcaa
      781 ttcacgccgc gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca
      841 ctacaaactc aaactgttaa tagtacaacc gcgccgcagc ccaactacat tccggggctg
      901 attgcggtgg tcgggtgtga tggcaccggt aaatccacac tgaccaccga cctggtgaaa
      961 tcgctgcaac aacactggca aaccgagcgg cgctatctgg ggctgctctc cggcgaagac
     1021 ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg actggcggcc
     1081 aaatcctcga aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt
     1141 atgtactgct tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa
     1201 agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat
     1261 gatggaccgg ggattggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag
     1321 cgcgaacggc gtttatacca acaaatggcg caatatcgcc cggaattaat tattcgcctg
     1381 ggcattgata ttgagactgc catctcccgc aagcctgacc atgactatgc cgagctgcag
     1441 gacaaatcgg cgtcatgtcg acgattggct ataacggcac aaaaattctt gagattgatt
     1501 ccagagcgcc ctacagcgaa gtgctggaac aggcacagaa ggcggtttct ctggttgcca
     1561 tcgtttctga ccgccgaagt ttaacttaag gctggcggga tactttttac tatcaacgat
     1621 aaggtcaggc tgaattggcg ggttttaaca tcaaacattg gtttgcagat ggcgcgtttc
     1681 gcaccattat tcgcaatagc gcctggttag gctccagtaa tgtcgtgagc gccttgctgg
     1741 gtctgttggc gctctcgtgt gccggtaaag ggatgacgcc cgccatgttt ggcgtactgg
     1801 tgattgtgca atcgtacgcc aagtcgatca gcgattttat taagtttcag acatggcaac
     1861 tggtggttca gtacggaacg ccagccttga ccaacaataa tccgcagcaa tttcgcaatg
     1921 tcgtctcatt ttccttctcg ctggatatcg tcagcggcgc ggtggcgatt gtcggtggca
     1981 ttgccttact gccattcctt tcccattcat taggtctgga tgaccaaagc ttttggctgg
     2041 cggcgctcta ttgcacgctc attccttcaa tggcttcctc cacgccgacc ggcattctgc
     2101 gtgcggtaga tcgcttcgat ttaattgctg tacagcaggc gacgaaacct tttctgcgcg
     2161 cagcggggag cgtcgtagcc tggtattttg actttggttt tgcgggtttt gttattgcct
     2221 ggtacgtgtc gaatctggtt ggcggcacca tgtactggtg gtttgcagcg cgcgaattac
     2281 gccgcagaaa tatccataat gccttcaaat tgaatctgtt tgagtctgcc cgacacatca
     2341 aaggcgcgtg gagttttgtc tggtcaacca acattgccca ctccatctgg tcggcgcgta
     2401 actcgtgcag cacagtgctg gtggggatcg tgttaggacc cgctgccgcc gggttattta
     2461 aaatcgccat gacattcttc gacgccgccg gaacgccagc gggtttgctg ggtaaaagtt
     2521 tttacccaga ggtgatgcgt ttagatccgc gcaccaccag accgtggttg ttaggtgtga
     2581 agtccggttt actggcgggc ggaatcggta ttctggtggc gctcgcggtg ttgatcgtcg
     2641 gcaagccgct catttcgctt gtgtttggcg ttaagtatct cgaagcgtat gacctgattc
     2701 aggtgatgct gggcgcaatt gtgatttcga tgctgggttt cccgcaggag tcattgctat
     2761 tgatggcggg aaaacagcgc gcttttctcg tggcgcaaac catcgcctca atcggctaca
     2821 tcgtattgct gttcatgttc tgtcatctgt ttggtgtgct gggagcagcg tttgcttact
     2881 tcggtgggca atgtctggat gtggtgctct cgttaattcc aactttaaag gcattttttc
     2941 agcgtcattc cttgctttat aacgcagccg gagagaaatc ctgatgatga gaaaatattt
     3001 cccgcttgag gcgagcgagc gtctatttgt tgcgattgag gaagatgatg tcgttgatgc
     3061 ccaggtcagt ttgccgccga ctattgcact aagttgtacc accgaaatca tccatgataa
     3121 ttacgcgtta tgcctgcagt tctggctcaa tggcgttaat cgtcaggagt tactgcgttt
     3181 aattcgcaaa cagaccaaag gcgatgagct gacgacagac gagcgcaaac agtttaaata
     3241 catgcgagcg cgctacaagc acctgcgttt tgcgcaacgg ctgtatttaa agaagcatca
     3301 ggccggattt ttattcggca aaacgaccgt ttttctgggg cgttttcagg acggctttcg
     3361 taacggtaag aaaaatattg tgtcgtatta cggcaacctg ttacggattt atttaagctc
     3421 tccggtctgg tcgttggtga actattcatt acgccatagc cagttagaaa gcgtaagcgg
     3481 ttttattgcc tatcggcaaa agcaaatgca taccctgaaa gagattattg ccaaaccgcg
     3541 actaaccggt agggaatttc atgatgtgcg aaaaatcatc agccagcagg tttcttatta
     3601 cgacacctta agatcgcttg atcctgaaaa taaagaggcg ttacagatat cccgcttcct
     3661 cgccgccatt aatggcctga tgggagataa acacgacgac atggtggcgg atgatatgga
     3721 aaaccgccag tcgtatgatg caccggtggc gctggatagc gatattcgcc agcgcttaga
     3781 gctgctgatt tcgcgttttc cgctataacc ggagcaatga tgtcatctcg tttgattatt
     3841 gcgctgatta tcatgctgtt agccccgggt gtgcaggcac acaatttcgt cactggcaaa
     3901 acggttaccc ctgtttatat ccaggaaggc ggcgagctgc ttctgaatag cgacgacgaa
     3961 attcactacc aaaagtggaa cagcacacaa ttggcgggaa aagtccgcat tatccagtat
     4021 atcgccgggc gtaagtcggc gaagaaaaag aactcactgt taattaaagc ggttgaagcg
     4081 gcaaactttc cgcaggatcg ttttcagccc accaccattg tgaacaccga tgatgccatc
     4141 tttggtactg gctattttgt tgttggcaaa atcgaaaaaa ataaacgccg ctatccgtgg
     4201 gcgcaattcg ttattgacgg caatggacag ggacgcgttg cctggcgctt gccggagcag
     4261 agttcaacca ttctggtgct gaataaagcc gggcaaattc agtgggcaaa ggacggcagc
     4321 ctgacgccgg aagaggtaga tcatgtcatt gcgttggcgc agaaactgat caatgaataa
     4381 aaaactcaaa ggattgaggg cgttagtgag atgaagctgg ttgaacacta catcatgcgt
     4441 ggtacgcgcc gtctggtgct gattatcgtc ggttttctga tctttatttt cgccagctac
     4501 tccgcacagc gttatctgac cgaagcggca aatgggacat tagctcttta tgttgtgctg
     4561 gatatcgttt tctacaaagt actgattgca ctggagatgt tgttacctgt tggtctgtat
     4621 gtgtcagttg gcgtgacgct agggcagatg tataccgact cggaaattac cgctatctct
     4681 gcggcggggg gcagtccggg acgcttgtac aaagccgttc tttatctggc gataccgcta
     4741 agtatttttg tcacccttct gtcgatgtat ggtcgtccgt gggcttatgc gcagatttat
     4801 caactggagc aacagtcgca gtcggagctg gatgttcgcc agttgcgggc aaagaaattt
     4861 aacactaacg ataacggacg aatgatcctt tcgcagacgg ttgatcagga taataatcgc
     4921 ctgactgacg cgctgattta tacttctact gccaatcgaa cccgtatttt ccgcgcccgt
     4981 tcggttgatg tggttgaccc atcacccgag aaaccgaccg ttatgttgca caacggcact
     5041 gcctatcttc tcgatcatca agggcgtgac gacaacgaac agatctaccg taatctgcaa
     5101 ttacatctga atccgctgga tcaaagcccg aacgtcaaac gcaaagcgaa atcggtcacg
     5161 gagctggcgc gctccgtctt tcctgccgat catgccgaac tgcaatggcg acaaagccgt
     5221 ggtctgacag cattgttgat ggcgctgctg gccatttcat taagtcgggt aaaaccgcgg
     5281 caagggcgat tttcaacgtt attgccactg acgttgctgt ttattgccat tttttatggc
     5341 ggcgacgtct gccgtacgct ggtggctaac ggcgcgattc ccctcattcc tggtttgtgg
     5401 ttagtgcccg gactcatgct aatgggcctg ctgatgctgg tcgcacgcga cttctctttg
     5461 ctgcagaaat tttcccgatg aatgttttca gtcgctattt aatccgtcat ctctttctcg
     5521 gttttgccgc cgccgcaggg ctattgctgc cgctttttac caccttcaac ttgattaacg
     5581 aactggatga tgtcagcccg ggcggttatc gctggactca ggcggtgctg gtggtgctaa
     5641 tgaccttacc gcgcacactg gtcgaacttt cgccgtttat cgcgttattg ggtggtattg
     5701 ttggactggg gcagttatcg aaaaacagtg agcttaccgc cattcgcagc acggggtttt
     5761 ctatcttccg tattgcactg gtggcgctgg ttgccgggat attgtggact gtttcgttag
     5821 gcgcgataga tgagtgggtg gcgtcgccat tgcagcaaca ggcgttgcaa atcaaatcga
     5881 ccgccaccgc gttgggggag gacgatgaca ttaccggcaa tatgctgtgg gccaggcgcg
     5941 gtaatgaatt tgtgacggtg aaatcgctga acgagcaggg ccagccggtg ggcgtggaga
     6001 tatttcatta tcgcgacgat ctctcgctcg aatcttacat ttatgcacgc agtgccacca
     6061 ttgaagacga caaaacgtgg atcctgcatg gtgtgaatca taaaaaatgg cttaacggta
     6121 aagaaacgct ggaaacatca gataatcttg cctggcaatc ggccttcacc agtatggatc
     6181 ttgatgagtt atcgatgccg gggaatactt tttctgtccg tcagcttaat cattacatcc
     6241 attatttgca ggaaaccggg caacccagca gcgaataccg ccttgcactg tgggaaaaac
     6301 tggggcaacc gatcctgacc ctggcgatga ttttgctggc tgtaccgttt acctttagcg
     6361 ccccgcgctc gccagggatg ggtagccgtc tcgctgtagg cgtcatcgtg ggcttactca
     6421 cctggatcag ctatcaaatc atggttaatc tgggattgct atttgcgtta agcgcacctg
     6481 ttaccgcgct cggtttaccg gtagcgtttg tgttggtggc gttgagcctg gtgtattggt
     6541 atgacagaca acattaaacc cgccgtggcg caaacaccac ggcgggctca cattactgct
     6601 tcagagtagc aaatgcctga atgatggcat cgatttgctc acgactgtgg gcagcattta
     6661 cgctacagcg cagcagcgtg atgcctgcag gcgcggcagg cggcaggata agattcacat
     6721 acacgcccag cgagataagc tcgcgccaga tgcgtaaacc ttcctctttc gaaccgatta
     6781 ttactggcac taccgggcta atatgtgaac ttaactcata acccagtttt tgcagaccgt
     6841 catacagatg gttggcgtta tccatcagct tttgccgcag ctctggttgt gtggcaatag
     6901 ttcttaacga cgagcgcacg gtcgcaatgc aggacggaga aggagaggcg gtaaaaatat
     6961 acgggcggct gccgtagcgc agcacttcca tcgcttccga accgacagca aaaccgccaa
     7021 tcgaggccag acttttactg aaagtaccaa cgataatatc cacatcatct tcaacgccga
     7081 cagcttcagc cagtccgcgc cccgttgcgc ctaacacgcc aaacgaatgc gcttcatcga
     7141 caatcaaata accaccgaga cgacgtttga tatcaacaat ttccgccagc ggcgcaacgt
     7201 caccgagcat gctgtagatg ccctcaacga tgataatcgc ctctttcgcc cgttcaccga
     7261 ggcgaaccat gcgccgttcc agatctttag catcgttatg gcggaaacga ataatttccg
     7321 cgccaccgag agaacaggcg tcataaatac tggcgtggct gtcggcatcc agtaacacca
     7381 cggcgttatg gtcggcaagt gcgctgatga cgcctaaatt agcggtataa ccggttgaaa
     7441 aaacgatagc ggtagggcga ttgaaaaact cggcaatctc tttctctaac gccaggtgcg
     7501 gcgcataact gccgtttgcc atacgagaac cggtggtgcc ggtgccttgg gccgctaacg
     7561 ctgcctgacc ttcgctgatg gcatcatgat taaaggttaa accgagataa ttattggtcc
     7621 cggccagaat cactttttta ttgccgatcc ggccttcggt ggcggaatac acttcatcaa
     7681 tacaggtgcc aaacggtgtt aacccgttgt cactaaattg cagtcgctcg ccagcaaggc
     7741 gcgcatattt atcgtatagc cccattattt attctccagc catggaggta gagtttccat
     7801 taattgcgca ggggttttga catccagcag aatattaata ggaatggaaa tatcgaatct
     7861 gtcttccagc atcattaaga gatccatcac tttgatcgac tccaggccaa gatcgttgac
     7921 gagatcgctg tcgggcttaa tttccacacc gttttcgacc aaatcctgta aacaggaaag
     7981 gatgtaatcc attactattt cacgatttac catagaaacg ttaacacacc tttaaacaag
     8041 acaatcaaaa tagcccgttg cgtaatgcgt gttccaggct aatcccggga aaccaattaa
     8101 tatcttcaga aatacggtta ttacttgccg accagtcggc gtgggttaat tcacgaattt
     8161 tcgagcgggt cagcataggt tctttacctg ccaggcgact caacgcggta ctgatatccg
     8221 caaggcaggt gagtaccggc agaggaatac caaccattcg cacggaaccg caacggacgt
     8281 tggcggcaag ttgctgtatg cgttgccagt catagccgcc agcgacgcca tcgcataatt
     8341 cataggtttg cgtctgtata gtttcggcgc ttaaccactg acccactgct tgcgcgaaat
     8401 cggtgacgtg cagaaaagag agctgtgtat ctggcgcacc aagtcgtggc agcaggccgc
     8461 gcagcatcca gtcaaacagc ggttttaact ctttatcgcc gggaccatat acggctgtcg
     8521 ggcgaaaaac gcccagcgta atttcgtcag ccattgcagt cagccgttgt tcggcgacgt
     8581 gtttggaatt tgcgtaccag gagagttcgg gatggcgcgc cgccagcgaa gagataaaca
     8641 gaaaacgttg gcaaaaaccg ctctcttttg ctgcttgcat caggcgcagg ctgccgtcaa
     8701 cgttacagcg ggtgaaaatc tcttctttgt gcccgcgcac ttgcccggcg caatggacca
     8761 ccgcgctggc tccggcaacc agcttgctaa gtgaatgagt atcttccagc gaaccgcgca
     8821 cccaggtgag attatcattg acgtgagcgc gggcagtacg cgtcaatgcg cgaacgtgaa
     8881 agccgcgggc gagcaggtta tcgataatat atttaccgat aaacccggta gcgcccgtca
     8941 ccgcgacagt ttgattcatg ccagggattc ctgcacatga aggcttgcag cataagcctt
     9001 ctgataacgt tttttcgctt ccgcacgggc aggcttgccg gaggatgttc ggggaatact
     9061 gtggggcggc aacagatcga tagccgctgt tacgccaaat tcgctttgga tccgtgctgc
     9121 cagcgcgtgg ataagctgcc cgcgacgttc ttcgtcgctg atccgacact ggatctgcaa
     9181 aatgattttt tcctgggcgg taacaaaagc aatcgcatcg ccagaatgaa tttccggttc
     9241 ttgttccgca atatattcaa tatcctgcgg ccagatatta cggccacgaa taataatcag
     9301 atctttaata cgtccggtga catacagata accgtccagc agataaccga ggtcgccggt
     9361 gtctaaccag cccgtcgcgg caatctcgtc ttgcgaaacc tggtcgccaa agtaaccgct
     9421 catcaggctg gggccggaga tgcaaatatg gcctaccaca cgttccgcga ccggcatacc
     9481 tgcttcattg cggatttcaa tgccatgttc cggcaacgct ttgccgcagt tgacgaaagt
     9541 cgatacggcg cgtgtttcag cacccggcgc gacggctttg ccctgatatt ctaggatgtc
     9601 gcgatccact tcgttaacca caaccccaga gccttcatca gagaagctga cagccagcgc
     9661 attttctgcc agtccgtagc acggcatgaa cgttttatcg tcaaagttaa cctgacggaa
     9721 acattcagcg aattgatgga gttgttctgc ggagatcggc tcagcaccaa taccagcgac
     9781 gcgccagcag gaaagatcca gttcagcgag atctttttca ttcacgcggc gctggcacaa
     9841 ttcatagcca aacggcggcg caacggaaac ggtgccgcga tttttactga tcaatttaag
     9901 ccattgcaga ggacgcatgg caaaatcctg agtgcgcaaa taatctactg aaagttgcgt
     9961 ggcgacgggg gtcaggagaa agccgaccag tcccatatca tggtagaaag gcagccagga
    10021 gacgcagcgg tcgccagggc gtaatttaat gccatcgtgg cttatagcac gtagattagc
    10081 catcacttcg cgatgggtga taatgacgcc acggggaaaa cgggtgctgc cggaggtgta
    10141 ctggaggtag gcgatatcgt tcggaactgg acgctggagc gcaacatcgg cttccggtaa
    10201 tgccttaaac caggcgtggc ttaaaacatg taattcgggg ttgttatgcg tcgcggcatt
    10261 gaccagtggc aaccactcat caccagtgat aatggctgcg ggctggcaac ttgccagtaa
    10321 accctggagt ttggcgctcc aggaatcccg ctgaccgacg cccattggaa tcgccaacgg
    10381 gacggcgact aagccagcat actgacaggc aaaaaaagcc tctacaaacc cgctactcgt
    10441 ttcggcaatc agcgccacgc gttcgccttt tttcaggttc agcgataaca accgcttcgc
    10501 accagcttcg gcacgcgttt ttaacgtctg atattccagt tgatcttcaa gttggcaacg
    10561 tctgtcataa aaattcattc cggcgctact cagagcggcg tagtccaaag catcaaccag
    10621 cgttggaaaa tcggcatagc gcataggtag ggaatgcgta aagattttat tagacatata
    10681 caccacgaaa aaattggata tcaaaaataa aatgacaata atgcggtgga ttatataggc
    10741 ataaaagata aatgagatag tattttcatc ggtgtgaaaa tgaacattga tttaaataat
    10801 gagtttaagt tttttgtttt tattgtgaat gatatttaat ttatgaatat attaagaaga
    10861 agccttgtcc ggtcgcctta aatcgacaaa a
//
LOCUS       DAZGCS010000138        10872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGCS010000138 DAZGCS010000000
VERSION     DAZGCS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.140"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2282)
                     /locus_tag="ACHK01_004277"
     CDS             complement(<1..2282)
                     /locus_tag="ACHK01_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO8099122.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKR"
     gene            complement(2500..3371)
                     /locus_tag="ACHK01_004278"
                     /pseudo
     CDS             complement(2500..3371)
                     /locus_tag="ACHK01_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            complement(3455..4606)
                     /locus_tag="ACHK01_004279"
     CDS             complement(3455..4606)
                     /locus_tag="ACHK01_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099123.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            complement(5874..6171)
                     /locus_tag="ACHK01_004280"
                     /pseudo
     CDS             complement(5874..6171)
                     /locus_tag="ACHK01_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(7001..7603)
                     /locus_tag="ACHK01_004281"
     CDS             complement(7001..7603)
                     /locus_tag="ACHK01_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099124.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(7697..7903)
                     /locus_tag="ACHK01_004282"
     CDS             complement(7697..7903)
                     /locus_tag="ACHK01_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099125.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(9030..9230)
                     /locus_tag="ACHK01_004283"
     CDS             complement(9030..9230)
                     /locus_tag="ACHK01_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099126.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            9430..9999
                     /locus_tag="ACHK01_004284"
     CDS             9430..9999
                     /locus_tag="ACHK01_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8099127.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            10259..10660
                     /locus_tag="ACHK01_004285"
     CDS             10259..10660
                     /locus_tag="ACHK01_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8099128.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVLDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            10648..>10872
                     /locus_tag="ACHK01_004286"
     CDS             10648..>10872
                     /locus_tag="ACHK01_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8099129.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAE"
BASE COUNT         2487 a         2784 c         2790 g         2811 t
ORIGIN      
        1 cgtttatgat cttcagagct ctgaatcagc gtgttacgca gttgttcaac tttctccctg
       61 aatgcgcaga aaaaggcatc gatttcctgg cggacgggct ggttaatatc ctgctcaatg
      121 gatgcctgca gctccccgaa atatgcccgg gtcagtgaca taacttcctc tctgaccttg
      181 ttgatattga tcacgctgcg actcacatcc tcgtcatagt tttcccagcc caggtcactg
      241 gtgccaaacc atcgacaaac ggttccccat acgccactct gccgacggcg acgggtgacg
      301 gtctccgttc tttcctccat aagcgcactc agatttattt ttaccgcgag ccgggtctgt
      361 aactggctga ctgccggaaa actgattttt acggtaaagc cctcatcctc caggcgggca
      421 ttgatgcgac cggcaatgtt ttctgtggcg tacagggccg tgccacgaaa acctgtttca
      481 atcccgctga cgattttctc cagctcaggc cggaactgtg cttcagcctc acggtgcagg
      541 ctggtcacgg tcgattcgat ttgcgtcatc agttcaaggg cttccctgcg atcactgaat
      601 tttatttcgg ggctgtccgg ggcaaaatct cgtttcctgt aatcctcccg gccctggttg
      661 ttgataccgg accattgaga aattttcccc cataatccgg actgagacct tcgttgttct
      721 gcgtcttctt cctccagcat ttgctgttct ttgcgtttac cgcttctgaa atagtcattc
      781 agctcctctt ccagtgaggt gcagaccccc gtcagtaatt cccgggtttt agtatcgatc
      841 tcctccatgg ccattctgac atcagtggtc atctgctcct gacagtccgc gatttcccgg
      901 atatccgcaa gcaggctggt gatatgcgac tgcagtgact gaatgctttg ctgtagcccc
      961 tgatggcgta acaacaggta ttcactgata ttttctgcat tctgcatcag ttttgcggcg
     1021 gcagagtcaa ccgccagtgc cgcagctctg gaatgtgagc tctgtatgac ttccgttatc
     1081 agttgatcga taagtgagcc ttcccacagg tctgtcgccc cttcagttgc cagttcactg
     1141 tctttacaga cgtattcttt tttcatgcaa ccgaaggcac ctctgataaa atcatcgacc
     1201 caggcttcat caacaggaag ggttccgttt ttccgtaact catgaagcgc acggttagcc
     1261 aggtatgcct ggcgtgagga tccgggataa acgcgggagg cgggcagcac atcgctgttc
     1321 agcattgccg gaactttctg gcgtacggca tctgccccat cgccattgcg gtctttctca
     1381 tcaaatttat tgaccagtac aaacaggcgg ccggctgata cgtcagcaat ggcattaagc
     1441 tctttacgga cgtcttcatc cgcttttgag ttcatctggg tgtaatccat cacagccaga
     1501 accgcagagg ctttctgcag ttgatcccgc atcatcactt ccatctgcgg ttgtccggcc
     1561 tcattagggc cgggggtatc cagcagtgtg agtgttccct ggcatgcatc catccccacc
     1621 agatggctga attccacgtc gatcaccggc agtttctgca cttctgcgta ttcatcaaaa
     1681 ggaaattcag tccccattgc cgcagcaaga cgaaccagat cgtttaacga tgccagtccg
     1741 gtaaagattt cctcctcccc gtggtattca tttttgagcc agccatcatc cgtcagaata
     1801 tccagcagtt cgcgggtatc cccggtttgc tgcagggtct gtgcgacctg ctgtcctgcc
     1861 ggggtggcga gtttttcctg cagtgtgatt aataaattgc ggacaggctg aatatgttcc
     1921 agatggagaa ccggctcagt ttttccggga acgtggcgga tgagcgtcgg tacagaggtc
     1981 atggggcggt tacggttagg cagaatttcc tgccccacaa tggcgttgat ggtggttgat
     2041 ttgcctgctt tcatcgtgcc gacaacagcc agcaccatct ccctgcgggc ggttttgatt
     2101 tgttcgccct ccagctcctc aattcttttc agtgctttct gtttatcaaa aagcagtctg
     2161 ttttcgttct tactgtcaga taacacatct ggctcctcaa ccatttgccg gagcagatta
     2221 atgttcagtt gcaaaagtcg gtcggcttca tcacaaagca gggcgatgtt cttttcgtgc
     2281 attatttaac cttcctaaga cgcattttca tgacaaccac ggaggaggtc atttactaca
     2341 aacgatcgtt tataacgatc gatatgatag taacgatcga tgcattctat taacttgaga
     2401 aacacaacgc aaggggacaa cccgggtatg gctgcgtttg catagtgttt tcatggagtt
     2461 aaaaatctgt aacaacagcc tggtacatca ccaccgggtt cagaagaaaa tccagttcca
     2521 taccgcgcgg gcaacagaga ccaccgagtc acggacggcg cgcagaaccg tgcgtgcaac
     2581 agaggcaatg cagacgctct ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg
     2641 ttcacgggcc tgagaccgga cagactccgt gcgcagctcg tcctgcaggc gggtcatcag
     2701 gggactggcg gcatggtcgg gaagtgctgt catgagcgca ctgaccagcg tatccagttc
     2761 ccagccggtg cgggccgata cggcaacaac ccgatgtacg ggccggaaca gacggaatac
     2821 cgcctccgtt ttttcgcgaa tgttctgtgc ctctgcggga gaaggctgaa taccggccat
     2881 atcccattca tggcagggct ccgttttatc ggcctgcatc accacaaaca gtacccgctg
     2941 atgtccccgg tgcaggatgt gtcgccagaa atactcatcc acagacaggg cacggtcatc
     3001 ggctttaatc agccacagta ccaggtccag ttcgagcaga atgtcacggt acagggcttc
     3061 atactctgca tccctgtccc ggctctcgcc caccccgggc aggtcagtga taaccatgct
     3121 gtgaccatgg ccattcagac ggaagcgctg cacttcccgg gtgccggcgt gaacatcact
     3181 gaccggggtg acctccccct gaaacagcgc attacagagt gaggatttac gggcccgctt
     3241 ttacccataa tgccaggcac gggttcgtga ctggtgagtt tgcgcagatg ttccaggatg
     3301 tgacgggaaa gtgagtaagg cagggaggag agcggttttt caattgcctc aatggcatca
     3361 gacggattca tacatgtatt cccggtgaaa aaagacaaaa ccccgcgcac cggaaaggtg
     3421 gcgggggatt cacggaaata atatctgtct gaaattatct ggaatcgatt tggctgttct
     3481 gcctggttgc caccaaatca tggtacgcct gctcaatcat ttcattcagc agtttttttg
     3541 ggaggacacc acgacttcgg gcaatacttt caagctgttt gtggattttt ccggacagtt
     3601 tcagagggac ggaaccatca gcacgttcat cacgcctttt ttgttgctcc cgggcacttt
     3661 tcagctcatc aagaagaatc gctttatgct gaatcctggg ggcctggaag gagacctgcg
     3721 taaacttcgc tctgttccgt ctgggattac tttccagtaa aaactgtacc tgttgctcat
     3781 tccagccctc ccagttatca aacgtggcgc aggcaaacca gtatttctgc tccggggtaa
     3841 ggggattcag cggggttccc acacaccgga cctgcatggc gttccataac cagtcacact
     3901 gttcactgtc cctgctgtcc acccacgaaa agggggaggg gtaatcattg tttatgctgg
     3961 cccattcaga gaatactgag ttgataatcg tcctcgtagc ctctggtccg gatttcagca
     4021 ggtaactgga gcagaaccat ataatttcac ggtagatttt atccggatgc acggtgcgca
     4081 tcggcttttc tctgggaccg gcaatcttcg gttgcgatgg aaaccccgtg gcaaaccgca
     4141 gatgttccgg gggcattcct gaacgttttt caggaatggg tgtgagtcct gcgtttctgg
     4201 tcttttccgc ttgtccggta ttgcctccgg ccggagtgtt gtcgggagta tcttccggag
     4261 gcgaggtgtt ttcattagtt gggggaacaa cagcagaact gtccatctcc ggaatgggtt
     4321 gaggggcata atcactgtgg tagccagggg aaagcgttaa gtcccgaagg gtgacatcag
     4381 gttccgggca gtgagtgcca ccgggagtga acgggagttg ttgcttttgg gagaaaaatg
     4441 tattgtgaat gcgggtatac agctggaaca gaaaaaaata agatgcccgt ggttcttttt
     4501 ttaaccactg tgtatattcc tctatggcac cgagctgttg tttgtgccgt tttacagcat
     4561 cgatatacgg acagctggtg cagatatcat ccagttcctt tcccacaata cacctcacct
     4621 gcttttctca catttgcaga ttttaaaaaa gcaaacaaaa acctccgtac atgatgtaca
     4681 gaacaatcta ccaggtagtg gcgtcagaaa acctccgggc cgcctgcact ttccggaatg
     4741 cgccggttat gaaaaaccgg aaattcagtc tctgttgtaa gatgtaccct ctgatactac
     4801 gttttctctg tgtatttaac tcactgtatt tattgtcttt ttaagcttga ctgatattct
     4861 ccggtcatag tgtgccggat acacatcccg taaacttctc atttttatat aaaaatacct
     4921 gcactctctt cagcgcatct gtctccttta agtaaaaaat gtttttacgt attgctgtaa
     4981 attcatttta aatcagtata ttaccttgat attgctgtct cctggtttga tgcgtaatcg
     5041 ttctgccttt ctggatgatt ctggtgcttc cgggtgcatt ctcctctgca catgcaacag
     5101 ttcgtcatcg ccacgctccc aaaaagcata taaaaacgaa tcgaatttga tatttttttc
     5161 aatcgttttt cataggaatt tcgtagggta ttctccgcta taacctccat atttgctgtc
     5221 tttttcttca taaatcgtat gcaatgtagc ctgtctgtca agacctcttc tctgctgttg
     5281 aggagacgat ctcatttttg gtcttttccg ggagagaggc tgtcagatga gcgagccgga
     5341 tatactgtca gaggtacacc ccgtagcaga cctgtgctcc catttccgcg atccggaacc
     5401 aacaacaccc tacggttaat accatttgaa gcgtcaggta ctgcaggaag ttcccccctg
     5461 cgcggacatc ggttcctgcc gacatctcac aatacggcca gactgtcatg cagtaacacc
     5521 cgcatgcggt ctgcggtttt aagcgcactc tcccgttttc agtgcaccga taatgcgtca
     5581 tagtaagtct gggggaagtt acggaatgcc tgttaagtgg tgccgtgggt ggagacagct
     5641 tacttttcta cagcaactgc agtcattcag gatgatcagg agatattctg tcaatgtcac
     5701 gatcctgcca ttttcgtacc cgctaagata cttccgttac tgatgctgtc cgcttttcac
     5761 catttcatcc tcactgacag gcgttgcgtt ttgggtcatc accacagctt tacgtttctc
     5821 ttttaaccgg taacgctctc ctttgatatt cagcgtggtt gagtgatgca gcagtcaatc
     5881 caggagcgct gttgtccggc aaccaccata tcaacttctt cccgggtgtg gcggagaggt
     5941 ggtatcctgg ctgagtttcg aagcgaaccg ttctttttga cgggcgcatt ttacgtccgg
     6001 gctgaatgta gtaacgcggc atggaacgtc ggcccgtata ccattgcctt aatctccgca
     6061 aagataacct cgtcattccg gacattctct gccaggcata tgtcgatgta atccataaac
     6121 gatttcagct tacccctttt gttggccagt ctttctggct ggcggttcag gggatgtttt
     6181 ctggttatcg tgtaccacaa agcagggata ttgccccgat agtcacattt cagattgatc
     6241 gcggtctgaa gatgtggcac tgaggtaacg tgctggtgtc actggtattg ctgaagttat
     6301 ggtttttcgg ggtcaggtga gcctgttgtg tgtatgcttt ttgttcaggt cagaggggga
     6361 ctgtgtggac agcggctttt gtctccgggc attcacgcct gaacgatgaa tgcgattttc
     6421 agcaatattt gtctttgtga cactctctgg ttgagaagct aatgcgcgcc cctgtttcca
     6481 cagagaatga ggcttgccat cagccgaaac atcagtattc cacgccatac agacggcgcc
     6541 tgaccatgtg ctgatgcagg taaaatgttt cactacaact gttcgagttg ctatgcaacg
     6601 aaacagcgat tctcacgatc tttaactgca acgtttttcc gcctgaaagc tgactgcctc
     6661 ctgctgaggt gggaggctat gattcagtgc gtttatagtc tccggggtat gcatgtaccg
     6721 gagcatacca tttctggtgg ataacaagcc ggcacattca gactgcagtg aagatggagg
     6781 cacctggttg tgaagaacgt ttctgcccgt ggctgcttcg catcatcaat ggtattcata
     6841 cccgctcaga aatgaccggg tgctgaagcc ctccgttatc gcgcagtata atgacgtata
     6901 tgatctgtgg acgagtgagc ggcgtgcctg atgtacgccg ctattactgt attttaccac
     6961 agggctggtt ttctgctgcc agccctgtgg ttctcagaca tcaactgtga gcggcatcaa
     7021 ccgatgtacg gtatgccata agcacgtact gatccatcag atttgtcagt ggttcactct
     7081 gaccctgacg tgcaatgatc cattgctcca gatctttttc tgatttgtgt ttcaggtttt
     7141 tcgtccagtc gtcaaggctg tttccggtca ttaagtcaag ccagcagagc cagaccagtt
     7201 tgagacgtaa ctccgtgccg ccaaactgac ggaagaggct gatcagtttg tttgccagag
     7261 aatgttgctt caggcgctca cattcagaca gcaggatccc ttccagcatc ggatgatgaa
     7321 ccagcaggtg ttcgctggtg cataaattca ccaccagatc atcatccagg gacggggagc
     7381 tccgtttgtc cggaccataa atcagccggt gcagactctg gggaacgatg gcaatctccg
     7441 gtttttcgcc attgaatgac acccacccgg gcgtcagatt atggaaatag tagatattcg
     7501 gatgacacac gccccatgat acggcgtgcc gggtgaagcc ggtcagccat ttctggtagt
     7561 tttcatggag cagctcgtaa cgggtctgat actgttgttt cattttcata ttcctcacgg
     7621 gggataacag gcagcagagc gccgccggca ggccggagat tgcgtctgct gtgggcgaag
     7681 ggttagtggt taaaaattac ggacgggact gtgcaatacg cgcctgcagc caggcttcca
     7741 cttcactttt cagccagcgg gagctgcgcc ccattttgat gggcgcagga aagcccccat
     7801 ctttgatgag tttgtcaaac cacttatcgg ttaggccggt cagttgagtg ataaacgcca
     7861 tgtcgaccat ctggtcatcc atcagcgaaa ctggggtagc catgagaatg ttcctccgga
     7921 tgttgttaaa ccagccggtg tggtcatgaa aacagcgcgc caccttcacc ggcataacaa
     7981 ccggggagaa acgtaaaaaa taatcgggaa tgcggctgtc cggagataaa cggggggatt
     8041 gcgatggcag agccgtagcg gaatcccgga ggggggtgtt gcgaaaagat gcttccgcac
     8101 gcacgcgcga taaagagggg atagttttcg tgaggacagg gagagaagca ggcgttctga
     8161 taattgcaga gttgttttcg gaggtggtta cagtgagtca gcccgttcct ggcgagcagg
     8221 agactgatat caccgatggg ataaggttgt ctgattggta ttataaatat aagaagtcat
     8281 cagagagtaa cgtaaaggat ttgccagaat aacctaagag agtaaatctc tccggtaaca
     8341 gagcgctgtg aagcattctg tatcagagtg cagatacatg aacgctgatg cgtaaaatca
     8401 gcagtgacac gccagctccc cctatgccca ttcctgtata agacatgaac tgagtgtatt
     8461 aatgcttctg ccctgagact gagccggtga gtgaggggct gagaaatccc tcttgacaca
     8521 gctctgacca gaaatacttc cccggttgtt gcggtgcgtc cggatccccg ataacggaac
     8581 actaaggctg tattgcggga caccgggtaa gtagcagccc gtaaggcgtt ctgtcaacgc
     8641 cagaaaaaat gatttcccac acgcgttata gactcactcc tgtggacctg gaagcccgta
     8701 actctgcggt tgcccactga ctgacggaac tgaccgcaaa ttttacgagt gcgtgctggg
     8761 cttcagtctg gtcacctgag cgttcggtaa cggaggcgcg ggcggtaata ggctgatttt
     8821 caggtattac cggcttacag cgtgtggttg atattgaggg gctgtgagtt tcgcgtattt
     8881 gtcagaaacc cagacgagtt ttcctgtatg ggacgaaata aggcagcggc aatacctgga
     8941 aaaatttaat atggtatgaa ggagaacctg tcggttacgc ttaatatacc cggtacactg
     9001 tcagctcagg ggatacatga accgttatct caccagctac acagtatata cacactgacg
     9061 ctgacagtag tcagcaggac cacaataatc agtgccagca gtatggtaac gatacggtct
     9121 gtattccggt aatagccgaa ggggtcgaca gaaccacaat acgggcaaca cactgtatta
     9181 tgagtaatcg cgtttccaca gtccctgcac ttctttttgc gtatcaccat cactccctcc
     9241 tcacagcctt acccgtaacc ggttttttac attaaaaaaa caacgctgaa aattcaaccg
     9301 tcagtccgga gacgaccgtt cgggttatca cagagtgcct gagacagtgt cctgccggag
     9361 gtcacttcag ggactctgcg cattttttac gacgtggcta ttccgtatga accatacgga
     9421 gatttaacca tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacggtg
     9481 cggcacgcac tgaatgttca tccccgcctg acggcattgc gggttgacct gcgtttcccg
     9541 gatgtaccgg cagcaacgga cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc
     9601 cgaatcgacg cttaccagaa acgtaagcat cgggaaggta aacgcgtgca tcccacaacc
     9661 ctgcattacg tctgggcccg ggagtttggg gagtgcaaag gtaaaaaaca ctatcacctg
     9721 atgctgctgg ttaaccggga tacctggtgt cgtgccggtg attaccgtgc tccgggatca
     9781 ctggccggga tgattaaaca ggcgtggtgc agtgccctgg gagtggatgt cgggtgccat
     9841 gccacgctgg tgcattttcc ggcctggccg gcggtgtggc tggaacgcga tgatgacact
     9901 ggctttcagc aggtgctgga acgtgctgac tatctggcga aggaacatac caaagctcac
     9961 tgcaccggtg agcgcaattt tggctgtagc cggagttgag cacgactggc gcgccaatac
    10021 ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg atgtatccac tcgcccactg
    10081 actgtttttt acgtcagtgt tccattgtgt gacaaccaca gtgctgtaac agcaaccagc
    10141 ctgctttacc caaactccag tccagatttt aactgaaccg ccattcacac cctgaccacg
    10201 ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg cggctgagga atatgactat
    10261 gtacgcaaaa tcctttatcg ctcttgatgg caacggacgt ctgacgggcg cccgtactgc
    10321 acaggccgca ccttatgcta actacacctg ccacttgtgt ggcagtgcac tcagatacca
    10381 tccgcaatac gacactgaac ttccctggtt tgaacacact gacgacaggc tgacagagca
    10441 cggtcaacag tgcccttatg tcaggccgga gcgcagagaa atacagttga ttaaacgtct
    10501 gcagcaattc gtactggatg ccttacccgt ggtgcgtaaa gccagctggc actgcagaca
    10561 atgtcaccac gattattatg gggagcggta ctgcacacac tgccagaccg gaggattcag
    10621 tattccccgg acaactcagg aggaaatatg cgaattctga actgttatat ggcgaatgac
    10681 agcaaaggcc attttgttac ggcgaaagaa gcagcgaagc acaaccgcca ggacgttttg
    10741 tgctgtgtgt cctgtggatg cccgttaaca cttcagcggg gcaatgacgg acaaccaccg
    10801 tggtttgaac atgaccagat gactgtcgct gagaaaatcc tgctgcgatg cacctggctt
    10861 gacccggcag ag
//
LOCUS       DAZGCS010000139        10663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGCS010000139 DAZGCS010000000
VERSION     DAZGCS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.141"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHK01_004287"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHK01_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHK01_004288"
     CDS             complement(655..1299)
                     /locus_tag="ACHK01_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099130.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHK01_004289"
     CDS             complement(1320..1589)
                     /locus_tag="ACHK01_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8099131.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHK01_004290"
     CDS             complement(1668..2306)
                     /locus_tag="ACHK01_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO8099132.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3522)
                     /locus_tag="ACHK01_004291"
                     /pseudo
     CDS             complement(2291..3522)
                     /locus_tag="ACHK01_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4023..6704)
                     /locus_tag="ACHK01_004292"
     CDS             complement(4023..6704)
                     /locus_tag="ACHK01_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO8099133.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6945..7991)
                     /locus_tag="ACHK01_004293"
     CDS             complement(6945..7991)
                     /locus_tag="ACHK01_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO8099134.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8095..8244)
                     /locus_tag="ACHK01_004294"
     CDS             complement(8095..8244)
                     /locus_tag="ACHK01_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099135.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8666..8857
                     /locus_tag="ACHK01_004295"
     CDS             8666..8857
                     /locus_tag="ACHK01_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8099136.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(8910..9143)
                     /locus_tag="ACHK01_004296"
     CDS             complement(8910..9143)
                     /locus_tag="ACHK01_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099137.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9239..9862)
                     /locus_tag="ACHK01_004297"
     CDS             complement(9239..9862)
                     /locus_tag="ACHK01_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8099138.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9951..10460)
                     /locus_tag="ACHK01_004298"
     CDS             complement(9951..10460)
                     /locus_tag="ACHK01_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099139.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         2930 a         2723 c         2163 g         2847 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagtacc
     1981 tggcgatgaa aaccatccac caccaagtaa tggttctttt cctcagacac taccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 acaggctcct gtgcaaaagg actgacctca tgaagcaccc tgcgcaggtg attaagggcc
     2221 tgtatggcct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaagt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc cttcatacgc tgcaggtaac
     2341 gttcatagtg ccggggtttg ttagggctaa aggagagggt ccggcaccag aaatcattct
     2401 ttatgagagt cttacagata cgtcgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cggaatgtca tccgggaagc cccgcgtctg ataccaacgc aggtagacag
     2521 caattttatt acggtaatgt tctgctgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgaggcggct tcgtgatacg cttacacagg gcaaaatagg
     2641 caccactctc attggcataa agcgccccgc tggcagcccc tgacacccgt tcacacatcc
     2701 tggcccaggt ttccggctcc agaacatggt aaagccacag tcccttacgc tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgcc ggcacggtac atcaggtcat
     2821 acagaggatt gtaaatggca caggttctgg cgttatatat ccagatatcg cgggccttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag ataccagtcc cataaagcga ttgagcgatt
     3001 catcagcacg gacaccagtc agtattgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gtaaaaggga aaatacgtca
     3121 tgtctgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt cagggaagtg ccacccagta
     3241 aaaggtttcc gtcacatcat ggtacatttc ccgcatcttc tgaatatgtt caatagtgca
     3301 ccgatactgg gcctcccagt caatgaacag aacagagaaa tgacgttttc tcctgcgggc
     3361 cacctctgcc acaagatgga aaagcacagt ggaatccttt ccacctgaaa aagacaggca
     3421 taccgacgga aatgtttcaa aaatccactc aatgcgggcg attgcggcac gcaaaacatt
     3481 ttctccggta aggatttttt cggttaaaag cgtatcactc attttcctgg ctttatgtcg
     3541 aataattctg tttatgcatt cagacaaact tcattaataa ctcaccgctt tggggataaa
     3601 taataacaat aaaactcttc agatattaat ctggttgttg gatttgttat cacatccttt
     3661 atccacctgc ttcttactgg taacacatct ggaaaatcca tgcattttca acatatacca
     3721 tcctttcaac ttgtaaataa ggaaatatga tgcagatggt gaacaattca aatatcattt
     3781 tgcaggaagt attcactgta atagaattac cccctgataa agtttattca ctaaggaatt
     3841 attcaacaga accattccct gttagctttg agcaatagca cttattttta tatgtatcag
     3901 gaatacgtac gttgatgtaa ccaaaacaaa gaagcctgtg tccatggggt tcgtacaatt
     3961 gtgtgtggca catgaccaaa tcaggtttga gacctcccgc tgcaatattt tttattcaga
     4021 ctttaacctg tcaggcctga cagaaacgct cccaacttcc cttctatatc actgtctata
     4081 gtacgtaatc ttagcaatgg caggccgcat ttcttcagta tactgttttt aagttcatca
     4141 cgttcagcct gaacactggt cagatgataa cctccatcca cttcaataac ccctaatgga
     4201 gtcttcccga ccttatagta aacaacaaag tcacaactgg cccggttacg catgaataac
     4261 tgttcacggt gtgtaaaatc cccccgctcc agcaacacca actgattcag cgctatttgt
     4321 gaatgaaaca tcatactccg ataactatcc tgcgacagga tatcccgcaa caggcaggcc
     4381 acaatctgtt ctgacttaaa gtgtgaatca tttgaattca atctactatt cagtcgttcc
     4441 agtgatttgt catactcact gtacagcaaa tcaaatgctg atatgaccgg gctctcgaaa
     4501 atctctccgt catcagcgta gtatttgatg taacgaatca gcgccgcaat atgtccagcg
     4561 tggcgttcaa atacatcatt accagtcacc agcgtaaatt tatttcgggc ccttgaaaca
     4621 gcgacattca ccagttcagg attatcaaca aacgcgatgt tccgactgtg ctggctgctg
     4681 cgcttcttat ccagcacagt ggaaaacaca atttcatcgc actcccgtcc ctgaaattta
     4741 tgcaccgtcg atttcacaaa atcagcaggc agcactgttt cagccagatt aacctgtgca
     4801 ttatatgggg cgatatatcc cctgctactt tcctcatccc agtaatgtcc ttccagagat
     4861 tcaagctccc tgagatttga aaaattacgg gtatggtttc cttttgccgt gatgatcaga
     4921 gataatgagg cttcacctga atcaagcgtt agcggtatca gagcattatc ataaaactgc
     4981 ttattacaaa attgaatgat tttaggatga cagcgataat gctctttaag aagagttact
     5041 ggcaccatat tcctgaacaa catacaaacc gaatcaagta gactatattt ctcacaatta
     5101 tagtattctg ccggtggaga tgggctgttc gggagtaata caggaacatg tggaagttgc
     5161 tttcgatccc ctacaacgat tacattacgg gcacatccca gtcccagaat gcccggaaca
     5221 atatcctgct gtgaggcttc atcaataatc acataatcaa gaagagcccc cttaccgata
     5281 gagttaataa tagaatgcgt gctgctacca ataaccggaa aacgctttat aaaacggtta
     5341 aattcatccc tgtatgtctc tgccgagaat gaatttcttg ttgaaatatt tcttcgaaga
     5401 tgttgtttta aataaagcac agaccatgat gtcaggcgct ccagtaaaat tttaaaatca
     5461 gattccttta atacttcctc atattcattc agagaaactt gtttttcccg gatgaatttt
     5521 tcataataag aaagctgaag agaagtaaat agtgccagcc ttctctccgg aatcaccaat
     5581 ggtttgactt tcagtatcct gaagtttaac aacaaatcaa ttctgtttct gaatccaatc
     5641 cgcctgtcag aaaggtaatg aatataagcc atcaggtcag ttgtttttcg tgaggaaagt
     5701 cggtatttgt gagaaaaaag ctcctccggt cgaagattct gactctgcca ttgctgcagg
     5761 tatttatact caatattcag ctcattaatt tctgctttta acgaagcgac ctggttaatg
     5821 gcattcaggt gttttttcac ttgttgaagt acatcatcaa tcgcatttgc agacggtgag
     5881 tcaggtaaga cttcctcaga acgcgaaatc gacgttgaaa aaaactgttg cctgttctcc
     5941 gtactcccca gtcgtgccac cacatatccc agttgctgtt tgtccatctt ttcataaaca
     6001 ttactgacag ctgagttatt attggacaaa attgccactg ttttattctg aattaatata
     6061 ttagcaacaa tattgagtat agtctgtgtt ttacctgttc caggtggtcc ttcaataact
     6121 gaaacctgtg aggaaaatgc ccgttcgaca gccagcaact gactttcatt aagaccaaac
     6181 ggaaaaatca aaccccatgg cgaatcacgt tttttactct gtcctgaaca gtatgcatgc
     6241 aaggcggtat ccggatgagg aactattttc tttatctgcc taaggatatt ctcatcgata
     6301 tttctgttat taccgcgggc ataaaaaata cgttcattag ccaccctaca caagtaatta
     6361 aacacggcat gttctgtaaa tgctgaacac tgttgaaagt caagaccggt cgttttcatg
     6421 atgtagggtc gttcaccctc gcgataataa acagcagcat atttatcacc atatataata
     6481 accagttcag cccgggatgt tacagcgttc cctttccttt cgaggaaaac atttcgcaaa
     6541 tttacagttg gcgttatctg acatacagaa agcggacgag tatattttct cccgctgcga
     6601 aaatgacagg tcagcataac ctctctgtcg tcatttccag cccacagcgt ccagtcggta
     6661 attttatgag tcttattttc accgtcaaca caaatcgtga ccatattctt actcagaaat
     6721 tatcatttaa cttacggaaa attgttaagg cactcccgat attagctccg gaggattttc
     6781 cacaactccg ggactgttaa catatttctg cctgccatat atacattgaa tttagtcata
     6841 acagtcaata ccagaattac aggaatgcga tgccgatacg ctggttattg aatactggta
     6901 gcccctgagc actaattcat cacgatactc tgtatttact gttttcactg ctttttacaa
     6961 ccattccccc aggtaaaatg ctgctcctcc ggtgatggct gtgtcagaaa ctgccatccc
     7021 ggaatagcca cgctgaccgc cagcaatgcc gcacagacac cagccacaaa cggtttcaga
     7081 aacgtgggcg gggatttctg aagggagggt gactgctccg cctgcacttt ccccttgcga
     7141 ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg ccgcaagcag cccttcccgg
     7201 ttatccacgc ttcccggcag actcagcgaa aaatcccctg ttcccgtctg aatcagactg
     7261 cctttacgat ttgacaggct ttgtgccgat atctgcagtg tgtcggcatt tatcgtaccg
     7321 ccattactgc tgaactgccc ggcagtccgg gcggaaagcg tatcggcact cagttgcgca
     7381 ttctgtgtag acagatttcc actggttgca ttcagctcag tatgttgtcc ctgtatccgg
     7441 ctgttaccta aatccagccc ctgctcaatg tcccgtaaat ccagcagaga accttcgtgt
     7501 gccgggaatg aggaatacaa ctgaatatag tcatcactgt ccggtgtcag acgcacatgt
     7561 cgcacaacac cgggaataat aaccagccgg aggataccgc tttttaaatc ctgcgaaggt
     7621 gccagaacac gggtggtgac ataaccatga tcgaccagac ggttctgcaa tgtactcatc
     7681 agcagattaa ttcctttcgc ccccaggcaa tgcccgaccg ccccgttggc gattttttgt
     7741 aaaggcagcc agtgtggtaa cgcatcagcc cccttcagtt ccgtctgttt aatctgaaaa
     7801 cagggcgttt caacaggaaa attaatctta tgggcaaaag agccaggtgc agaaaggcga
     7861 acatcagggg ctgaaggcgt cagttgttgc tcaagggctt cctgacgctg ctgctgaatg
     7921 ataaatgctt catctccgct acgccggaca tccgctgcct gaagagataa aggcgataat
     7981 aaagcagaca tcagtgtcag tgacagcaag gacggacagc gccggaataa gctttgttcc
     8041 tcagtcctct cgttcctgca acataatttt ccagtcaaac gaaaaacctt ttattcatgt
     8101 cataccaaca atcaccagcc tcctgacgat acctcccacc atgttttgcg atggcacgct
     8161 accgcccttc cggtatttac ccgcatcaac cggtaccaga atgctgagta acaaaccagc
     8221 tagaattgac ttaattttga tcattataga ataatgatct gaactacagc aacatgacag
     8281 atttatcgat cgatataaca caaatgaaca gttctttatt ctgacaaata tcgcaaaaac
     8341 agcacattac aaaatttata accataatta ctggaaaagt gcagaaaatt attccactga
     8401 atgcttttaa tacagttggt tagcttaaaa aatagcaatt tttgatttca gtcattgaaa
     8461 gttgcaatgt ttgacgattc aacagcattt aagtgagttt caggatatgg gaaggacatg
     8521 aaaggaggag cgcaataaaa tacacaggaa gaaggggaaa atgtttcgta aaaacagaaa
     8581 attaacagcg cacatcagga aacgcagtct cctgattcag tgctaccaca tgaaaattta
     8641 gttaactgtt accgtactaa aatttatgtc agaagatgtt ccattaccga aagtcaatca
     8701 gcgatacaaa gacgatcatg gtgcgcttgt caccctgacc agtgttgagg aaacccgggt
     8761 ggtgtttatg caggacggct atccgcatcc ctgcatgcga ccgatgtaca actttctggg
     8821 aaaattcaaa cccgaacccc gggaagaaac agaataaaac gaaatccctg ccggcaatca
     8881 cactacctga tagcagcata atccacttat taattctcat ccccggagac aagcccagaa
     8941 aaccactccg ggtgttcact gtatgccgtg ttcagcgcat ccctggaaac gtttatataa
     9001 aagctgtcct gcaatttgcg ggtaacaact tctgccaggc gattccggtt atccaccagt
     9061 ccggcataac ctctctctgc cagccactca ttcagttcat gttccagaga cagattgata
     9121 atatcataat cggaccttgc cattcccgtt tccttagcac atgttttcag gcacggggat
     9181 aaagtataaa gaatgtcata ctgctgctgc cagagcaata ttccccctga tggttgtatc
     9241 agagatggta tgccggtact ttgcgggttg ttcccgtcag gttatcgtgc acctccacgg
     9301 tcgtggtcac caccggcatt ccggcctttc tcagcctcag aacatcagcc gcaatacgct
     9361 gactgccaaa ccagaacagg ccgtccagtg cagtcaccag taaacccgcc tccagtgcat
     9421 acttcagccg ttcacggggc gctttcactt cccgggcaac ctgctgatat ggcaatgatg
     9481 tgttttcatt cccaaccacg cgacgaatac ggtgagacag atgataccgg tatgtatccg
     9541 gcacaccaga aaggctggcc ttcaggctgt acacgcagcc aaaccgttta tcgttaaaca
     9601 ccacattttt ctggctgatg ccccattctt cacgcagcgc ggcaatcaat tgcggtgtac
     9661 gggtaagcag caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg
     9721 caatacgcaa ccgttcttct ggcccggcct ctgtctgccg gcactcctcc agaacatcct
     9781 gccactgccg gcgaagacgg ggggattcgt tccgttctgt aaaacagtat ttatccgcca
     9841 gatagtcaat tcgtgtacgc atactgaaag gaattccgta taaagattca gcagacaaaa
     9901 ctttatcagc cagtaaaaat taacggaagc attgatattc ctcccgacaa tcaccggatg
     9961 attgttgcaa taacctgcga cataaaggac tgaacagccg tttttaacgc cacgtattgc
    10021 cctgatgaac caggccgggc cacccgaaaa catctttcca cccggcattg tccgccagca
    10081 cttatcaccg accaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc
    10141 atgacgccga ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc
    10201 tgtgctcctt catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc
    10261 gtgtgattat gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc
    10321 tgaatctgtt gaccatagat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga
    10381 tttggccagt ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt
    10441 aaggcttcgg ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc
    10501 ggtgcagaca cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac
    10561 aaacaataag aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag
    10621 aatgttactg acaatactac caatacttaa atagcaatat att
//
LOCUS       DAZGCS010000140        10562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGCS010000140 DAZGCS010000000
VERSION     DAZGCS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.142"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(518..658)
                     /locus_tag="ACHK01_004299"
     CDS             complement(518..658)
                     /locus_tag="ACHK01_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8099140.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(960..1223)
                     /locus_tag="ACHK01_004300"
     CDS             complement(960..1223)
                     /locus_tag="ACHK01_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8099141.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1646..1771
                     /locus_tag="ACHK01_004301"
                     /pseudo
     CDS             1646..1771
                     /locus_tag="ACHK01_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(1892..3505)
                     /locus_tag="ACHK01_004302"
     CDS             complement(1892..3505)
                     /locus_tag="ACHK01_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
                     /protein_id="HFO8099142.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQESDRYS
                     GREDDPLVPRQLRQSRHRRPLPAHLPREIYRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYSYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLRPFRGILQADAF
                     AGYDRLFSAEREGGALTEAGCWAHARRKIHDVYISTKSATAEEALKLIGELYAIEHEI
                     RGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFC
                     DDGLAEADNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(3536..3886)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004303"
     CDS             complement(3536..3886)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8099143.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(3883..4317)
                     /locus_tag="ACHK01_004304"
     CDS             complement(3883..4317)
                     /locus_tag="ACHK01_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8099144.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            4403..4645
                     /locus_tag="ACHK01_004305"
                     /pseudo
     CDS             4403..4645
                     /locus_tag="ACHK01_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4695..4934
                     /locus_tag="ACHK01_004306"
                     /pseudo
     CDS             4695..4934
                     /locus_tag="ACHK01_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4982..5599)
                     /gene="ureG"
                     /locus_tag="ACHK01_004307"
     CDS             complement(4982..5599)
                     /gene="ureG"
                     /locus_tag="ACHK01_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO8099145.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(5611..6285)
                     /locus_tag="ACHK01_004308"
     CDS             complement(5611..6285)
                     /locus_tag="ACHK01_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO8099146.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(6286..6750)
                     /gene="ureE"
                     /locus_tag="ACHK01_004309"
     CDS             complement(6286..6750)
                     /gene="ureE"
                     /locus_tag="ACHK01_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO8099147.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(6760..8428)
                     /gene="ureC"
                     /locus_tag="ACHK01_004310"
                     /pseudo
     CDS             complement(6760..8428)
                     /gene="ureC"
                     /locus_tag="ACHK01_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
     gene            complement(8454..8774)
                     /locus_tag="ACHK01_004311"
     CDS             complement(8454..8774)
                     /locus_tag="ACHK01_004311"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO8099148.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(8783..9085)
                     /locus_tag="ACHK01_004312"
     CDS             complement(8783..9085)
                     /locus_tag="ACHK01_004312"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO8099149.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(9095..9874)
                     /locus_tag="ACHK01_004313"
     CDS             complement(9095..9874)
                     /locus_tag="ACHK01_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO8099150.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            10255..10530
                     /locus_tag="ACHK01_004314"
     CDS             10255..10530
                     /locus_tag="ACHK01_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099151.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         2499 a         2742 c         2688 g         2633 t
ORIGIN      
        1 ttttatcaaa acatcaccag aaactggaag tgacaaccgc aataatgaac ataaaaaggc
       61 aaatcagaat ggcagtaaat caataaagta ccatttcata tgaatcatca gaaaaacggt
      121 ggacgcgaca tccagcacga ggcaaaacac agcaacaaaa taagccggat atcgcgccct
      181 gtacttttct aactcaactc ccggcattgt gcaagtttta atcgcaggtg gattcagagc
      241 cacgccccca ttgattcagc ctgaagacac gtatatcctt ggagaaagca ggaatctcat
      301 tgctgtgaaa ttccttacag aatcttcagc cccattttat ggggcttttt ttggtgcccc
      361 cacgctgtta ccacactttc gggatgcgaa taaaaaagcc cacagttttt acactgcgga
      421 cttttgtttt ttctttgtga tttgtgtgca agggtgcgac tttcgaccga acctttggca
      481 accggatagc ggggactgtt gtccccgctg cggtttctta cggtttacac tgcaagaacg
      541 ccgcaatttc cgaccacatg aagcttaccc tgaattcaca cggtgatgcg tgtaacaccc
      601 agacggtcag caacatggtc aggcataaca tgcccacaat gatcgttttt tgcggcatag
      661 cacttgctcc ttttacagag aggcgctaac cttccacttg ctgaaggtag atggtcaggg
      721 cctcgggtta acgtttaagt tgactcgggg cctttccaca tccggccttc aggtaatcct
      781 tccagccgtc agccgaaagg cacccgcgcg taatgtactg gttttcagag aggtccgcaa
      841 tcttccggtg acgagatcat ctgcacacgc cccggtggga cgggctaacg gtatattcag
      901 gtgcaggatc tctctgtctg gtgcgttttt aaattgtcaa acgcaccctg gcggcaaaat
      961 cagccacctt ttttcgcatc aacaaaacgc ccaaaacgct gggtaaagcg tgcaacatta
     1021 ccttctgaag cgacagttct cagtttacct gtataaaacg gatgcgactt agaagaaacg
     1081 tcaattgtaa catatgggta agttacacca tccagttcaa ttacccggtc agttttgatc
     1141 gtcgaaccga tcctgaagta ctcatcaata ctggtatcgt gaaatactac tgtacggtat
     1201 tccggatgga tattcggttt cacaacagtt ttcccccttt aagtgaaaat tccgcataaa
     1261 ggtacatatt cagacggtca gattcaactg tctgttctta taacctcact ctctttcagt
     1321 attacaatat gttatataat tataaactgg acttcccgaa aaaatccatt aaataagaag
     1381 cgagccgttg cggaggcttg ctctctcctt taacctcatg tgcaatatcc gtaacgcgct
     1441 cacataaatc atcatctgat aatataacaa cacccattat tttattctga cctaaaaact
     1501 tcttatgaaa taaacgttta cccgacgtat ctgatcccgc tgtttttacc tgtatccttt
     1561 aacatacttg agaagcccgc tgaaaaatat accgtaaagg cggttctctg tcttccataa
     1621 acctgtcgga taagaagggc attaaatgaa aaccaaaaaa gttatgatga ttgcactggt
     1681 cagctctgcg ctggctcttt caggatgtgg agccatgggc acagcgatca aaaaacgaaa
     1741 tctggaagta aaaacccaga tgagtgaaac ctgaggtagc ctgagtttaa cggacactcc
     1801 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc caacgttact
     1861 cgtaagcgtc aacggagcac cgtattgacg cttatttatt agtgagtact acgttccatg
     1921 gcaggagttc gtcaactcgg ttggaaggcc attccggcag tacgctcagg atatggcgca
     1981 gatacgcttc cggatcgata ccattcaacc ggcaggtgcc gatcagcccg tacaacagtg
     2041 caccacgctc gccgccgtga tcgctaccga agaacacgta atttttcttt ccgagacaga
     2101 ctgcacgaag cgctctttcc gctgtgttat tgtccgcctc cgccagaccg tcatcacaga
     2161 agttgcacaa cgcaacccag tgcttcctga tataccggaa cgcatctctc agacggcatt
     2221 ttttcgataa cgtgtgttct ttctcctgca tcagcttata cagggaagtc agtagcggtt
     2281 tactctgcat ttgcctgacc gccaggcgtt cagacaccgg caacccgcgt atttcgtgct
     2341 caatggcgta cagttcgccg attagtttca gtgcttcttc cgccgtcgcg cttttggtac
     2401 tgatatatac atcgtggatt ttgcgccgcg catgagccca gcatcctgct tccgtcaacg
     2461 cgccgccttc acgttcggca ctgaacagcc gatcgtaacc tgcgaacgca tctgcctgca
     2521 ggataccccg gaagggacga aggtgttgct ccggatgttt accctgatgg tccggtgagt
     2581 aggcgaacca gaccgccgga ggttctggcg aaccggcatt tcggtcatcc cggacatacg
     2641 tccagatata tcctgttttc gccttcttcc tgcccggtgc cagtactttt actggtgtgt
     2701 catcagtgtg aaccttgcgg gtgttcatca cataactgta cagggcatca ttcagcggcg
     2761 tcattaactg gcagcacgca tcaacccagt tggagagtaa ggcacggctc agttcgacac
     2821 cctggcgggc aaagatttca ctctgacgat acagtggcag gtgttcgcag tattttcccg
     2881 ttaacacgcg ggcaagtaac cccgggcccg cgataccacg ctctatcgga cgggatggtg
     2941 ccggtgcttc aacgatgcag tcacattttg tacaggcttt ttttacccgt tctgtgcgga
     3001 tcactttcag ggcgctgctc accagttcca gttgttctgc gctgacttcc cccagataat
     3061 ccagctcacc gccacactcc gggcaacagc tttcttcagg ctccaggcgg tatatttcac
     3121 gggggagatg tgccggtaac ggacgacgat ggcgcgactg tcgcaactgg cggggaacca
     3181 gcgggtcatc ctcacgccca ctgtaacgat cgctttcctg ctcgcgttgt ttcagcaggg
     3241 cttcagcctg ttcaacctca cgacgcagtt tttcagaacg ggttccgaac agcatccggc
     3301 gcagtttttc tatctgagcc cgcagatgct ctatttcccg ttcatcttct tcgatctttt
     3361 cttcggcacg tgccagtgca gagcgcagga aggcctccgt ctcttcaacc agactcagtt
     3421 gctggtcttt ctgacggagg gcttcagcct gctcagagag cagcctttcc agctcagcga
     3481 tgcgaatgag gtatttctga ctcatggccg tttttataat gcggtcagga gttttttaca
     3541 acattgtcag tgcgttaagg cgggatgttt ttggctgacg ccagtccagc ttatcgagga
     3601 gcattgccag ttgcgagcgg gtaatggata tcttaccgtc acgcaccgca ggccagataa
     3661 actgaccttc ctcaaggcgt ttggtgaaca ggcacagacc atcagcatca gcccacagga
     3721 ttttaatcgt gtctccccgt cggccacgga agataaacag gtgaccggag aaggggttat
     3781 catccagcac atgctgtatc tgctccccca gaccgttgaa ggatttacgc atatcagtaa
     3841 cgccggcaac gagccagata cgggtacctg atgggagtga aatcatcgtc ccctcccggt
     3901 cagctcacgg atcaacaccg tgagcagctc tggtgaagga ttttccagca tcattttacc
     3961 gtaatggaat tccacatggc aggaagctgc acaagcaact gataatgcct ccggagcagg
     4021 agcatctgta gcacagcgcg gggtgtcgat aagttccact ggaaccagag tcggagaggc
     4081 cggaaaagat tgtgatactg tagggcctac aatagttgga ggcattctac gagaaatacg
     4141 tccttctctt tgccagaggc gtagccattt aaaaatcagg ttgttatcaa cgccatgttc
     4201 ccgggccagt tgtgcaacat tggcatttgg tttcgaagcc agttcaacca tatgaagttt
     4261 aaattcatta gaaaatatcc ggcgggttcc ggagcgccag tctgtaggtt tatccatcaa
     4321 tagaactccg tctaatttag aaggagctct aatttagtct gcaaggagca ggtgttacag
     4381 acggcattgg ctttacgctt accgttactc taaagagttc aaagccgaag ctgtcagaac
     4441 ggttcttgaa aatcaacttt cgatcagtga aggcgcttcc cgattatctc ttcctgaagg
     4501 cactttagga caatgggtta ccgccgccag aaaagggctc ggtactcctg gttcccgcac
     4561 ggtggctgaa ctggaatctg aaattctgca actgcgtaag gcgttaaatg aagctcgcct
     4621 tgagcgagat atattaaaaa aagcagctgt agattcaatc tgtcaatgca acaccccttt
     4681 caattatctc tttcactgcc cgcgacggta tcggttatca gtttgacctg ctggctaacg
     4741 gtgaaaccgt actgcgtgat gcatccggcg aaaaacgcat caagggtgaa aaagacggtc
     4801 gtatgctcat gaatgttggc ctgaatgcgg aaattcgcga caacatccgc ttcggtctgg
     4861 agtttgaaaa atcggcatct ggtaagtaca acgtggataa cgcggtgaac gccaacttcc
     4921 gttattcctt ctgataaaaa caaggggccg cctgccggcc ctgatacaga ggatgttgtg
     4981 attacttacc cagcatgccc tgctcttcga taaaggcgat gatgttttgc agaccttcac
     5041 cacttttcag gttagtgaac ccccatgggc gttctccgcg catacgtaaa gtatcacgct
     5101 ccattacctc gagggaggcg cccacgtatg gtgcaaggtc agttttattg atcacgagga
     5161 agtcggattt ggtgatcccc ggaccgcctt tacgcgggat cttttctcct tctgccacat
     5221 ctatgacata tatggtaagg tccgccagtt cggggctgaa ggtcgcactc aggttgtcgc
     5281 cgccgctttc cacaaagatc agatcgagat taccaaattt ctcactcagt gcttccacgg
     5341 ctgccagatt cattgatgcg tcttcacgaa tggctgtatg agggcagccc cccgtttcga
     5401 caccgacaat gcgttccgct tccagtgctc cggcctcagt gagaatgcgt tgatcttctc
     5461 tggtgtagat gtcgttggtg actacggcta attgccaggt gtcacgcatg gatttgcaga
     5521 gggcttccag aagcgcggtt ttacctgagc cgaccggacc gccgacgccg acacgcagcg
     5581 gttgcttgat tatgttcatt ttattttctc ctaagaacgg aataatcggg aatactgggt
     5641 ttcatgtcgc gcggaagcga tagatgcaag tggcgttgct gaaccaattg cgtgatccgg
     5701 ggttgcaagg gcactgtcca tgttcgccgc atagcgggca cacaggcgca taattaactg
     5761 ctgggcagcc agctggccgt agggtaccag cctgacgccc gccatcactg cgctttcaat
     5821 ccagctgtag ccaaggctca gggccaggtc tgaaacggat atttgccagc gtacgccgag
     5881 ccatgccata ccggttaact ggctttgctg gcatagtttg cgccattcgg ccgggcagtc
     5941 gggctgccag tcgaccagaa gtctggtgaa ggctgcaccg cggttacgtt cttcatcgcg
     6001 tagctcacgg gtttccctgc aggccagcag ataagcggtc cagcgcccgg cactgtcagg
     6061 atcgcccgct tcacaggccc ggtatagccg ggcaagcagt ggcaggtcga cggcaaaaaa
     6121 actctgttcc atctgcaact gctgccagtg ttcaaaggcg gcgctatctt ctacccagcc
     6181 tgcttccact gcccactcca gtccctggga ccagctgtag cctccaacag ggaggctgtt
     6241 actggcaagc tgcatcagcc gcagacgttt ttccggagtc ggcattcagt ggctgtgctc
     6301 ctgatcgtga ttatgggcgt gcgacttgct ggcgtaagcg ccagcttccg gctcaaacgg
     6361 aagatgtgca aaatcaacat ccagcccaaa ctggcgcagc atatcatcga ggacgtgatc
     6421 gtgatggtag cgcagctctc cgggcattat ttgcagcgga acgtggcgat tgccgagatg
     6481 ccagcaggct ttcgcgagca taaaaggatc tgcgcagcgc actacagaaa cggcttcatc
     6541 agcggcgatc accttaatga attcatcacc gttttcgtta ctgagaatat caccatcgcg
     6601 cagcagcaga ccgcgtggca gcaacagtcc agcctgacgt ccatcgttaa gggtgacttt
     6661 aatacggctt tttacccgca tgtcaacagg gagggtaacg ctggcagttg tctgagccgg
     6721 ggtctccacc cgccgggtca aataaagcat ttgtgctcct taaaacagga aataacgttg
     6781 cgccattggc agaatgcgcc ggttcactgg ttatcagttc gccgttgata cgcacttcgt
     6841 aggtttgtga atccacggtt atatcaggca acaggctgtt gtggcgcata tcggcttttt
     6901 gtactgtgcg gcagcctttt accacagcag ttgtgctgtg caggttaagc tgttcagcga
     6961 cgccatttgc tgctgctgcc tgcgacagga aagtcacacg acagcggtga cgggcactgc
     7021 ccaatgcagc gaacattggg cgatagtgca ccggctgcgg tgtggggata gagccgttga
     7081 tatcacccat cggcgccatg gctatcattc cgcctttgac gatagtcgcc ggttttacgc
     7141 caaagaacgc cggggaccag agcaccagat ccgccagttt tcccacttca atcgagccga
     7201 cttcatgagc aataccgtgg gttaatgccg gattaatggt gtatttagcg atatagcgct
     7261 tcacgcggac gttgtcgtta tcaccacttt cttccggtaa cgggccgcgc tgaactttca
     7321 tccggtgtgc cacctgccag gttcgtaaca ctacttctcc gacgcgtccc atggcctgcg
     7381 aatcggacga ggtgagggag aacgcgccaa ggtcgtgcag gacgtcttcc gcggcaatgg
     7441 tttcctggcg aatgcgcgat tcggcaaagg ctacgtcctc ggcgatatcc gggtcaagat
     7501 gatggcaaac catcagcatg tccagatgct catcaatagt gttgacggta tagggtagcg
     7561 tcggattggt tgaggaaggc agaatattgg ggtgcgcgca ggcggtgata atatccggag
     7621 catgaccacc acctgcacct tctgtatgga aggtatggat agtgcgcccg ccgatggcag
     7681 tcagagtatc ctcaacgaat cctgattcgt taagcgtatc actgtgtagc gcaacctgaa
     7741 cgtccatttc gtcggctaca gtcagtgcgc agttgattac cgcaggtgta gctccccagt
     7801 cttcgtgaat tttgaggccg ataaccccgg ccgcgacctg ctcacgaagc gcatccggat
     7861 tggagcaatt gcctttaccc agcaacccga tattgaccgg caggctgtct gcagcctgca
     7921 gcatttgata aatgtaccat ggtcctgggg tacaggttgt ggcgttagaa cccgctgtcg
     7981 ggccagtacc gccaccgatc atggtggtaa tgccggatgt cagcgcttct tcagcctgct
     8041 gtgggcagat ccagtgaata tgcgtatcga cgccacctgc ggtaacgatc ctgccttctg
     8101 cggcaataat ttccgtggat acgccgattg ggatagtgac gttgggttgt atatcaggat
     8161 taccggcttt gccgatagca aatatccttc catctttgac gccgatatcg gctttaacga
     8221 tcccccagta atcgatgatc agggcattgg tcagcaccag atcagcgcag ccggcggaga
     8281 gcatttgccc ctgtcccata ccgtcgcgga ttactttacc gccgccgaat ttgacctctt
     8341 cgccgtaggt agttaaatca tcttcgacct cgatccacag ctcagtgtct gccagacgaa
     8401 ttttatcacc ggtggtaggg ccgaacatgt cagcataggc ctagcgtgaa atattactca
     8461 tcatttgcct ccagctcacc cattatatct ccgcggaaac cgtgcacgat acgggcaccg
     8521 ctcagcgcca ccagatcaac ctcacgcttt tggcctggtt cgaagcgcac tgcagttccg
     8581 gcggcgatat tgagacgata gcctcttgcc ttctggcgat cgaacttcag tgccgggtta
     8641 acctccgcga agtgataatg ggaaccgacc tgaattggtc ggtctccgtg attttcaacg
     8701 ataatactgc aggtagcccg gccgatgtta agctctatat agccaggttt aaccttatat
     8761 tctccaggga tcatctgtta cctcagatta tcggattatg gacggtaacc agcttggagc
     8821 cgtccggaaa ggtggcttcg acctggatat cggggatcat ttctggtatg ccttccatga
     8881 cctgctcgcg actcaggaca tgccgtcctt cttccatcag cgcagcgacg cttttgccgt
     8941 cgcgagcgcc ctccattata aaagcgctaa tcagggccac ggattcggga tagttaagtt
     9001 tcaggccgcg ggccagacga cgctctgcca gcagtgcagc ggtaaacagt aatagtttgt
     9061 ctttttctct tggagtcagt tccatagcgt tctcttaagt cagccagatc cggggaagta
     9121 ccggtgattt gtcagtcagt tgcggccgca taaattgcca gatatcacgc attacctgct
     9181 ggcaacgcag attatcatca cttaaaaagc gaactgtcag tagaccatcg gtgagcgtgg
     9241 ctccggcgta gttttccagt ggtgtgagtt tttcgcgtat gtcttccagt tgcgtttcat
     9301 tccccaggta gaacagcatc gttcccaccc atggtttccg ggcaacgcag gtaagttctc
     9361 cattggccac gtgcagacgc tcaatcaaca gcggagaacc gtcgacccat atctccagac
     9421 ggttagcgag tgttccgtgg ctgaaggttt ctccgatgac cggacgtcca aggcacagaa
     9481 gatcccaggc cagcagtgta ctggaggacg acagatgaaa cacggtatgc aacgtggcgt
     9541 gagcgccggg aaaaaaaatc gaatcctgtg gcaaccactc cagcgttgcg ttattgcaca
     9601 gcgtcaggtt ttgctgaagg tgcgcctgcg cgccgctgct gcggtaaaat ttactggcac
     9661 caggcatggt gatcagcgta tggcaatcag agtcaattat tgcgttgatg atgagttcgt
     9721 caccgccgac gataccaccg ggtggatgaa gcagacagag gtggcaggtt tcttcttcgg
     9781 gataaaaagg acgctgaaca gtgagcggac cgacatgatg acgggtggtg agcgtggttt
     9841 tcccgtgcag acgctgaaaa cggagatcca gcatggcctg ccagcctttt ttattttgtg
     9901 gtaacgcggt actgagcaat cttacgcccc tgataaataa agataaaacg acagtaaaga
     9961 gccggatttc cttgttatat aaaagatgat gcctttccag tgagttttcg tctgaataat
    10021 acgaatgttt gttttcgtca tgaagcgttc cggctcccgg ggctatacat cgccagaaaa
    10081 gcaatgcata ttgtcgggat tatccagaga atcaggcaga aagatccgac aatgggctgt
    10141 gattatgcca tctggctcag aatgatatca aggtgttatg gggataactt gctgatacaa
    10201 tagcagtatg acagattcat taatgcggaa actgctcact gaaacagagt gttcatgact
    10261 gagcactggt cgggtcatgc agatgataat ttcccctttt cttggggaag cgacagtatt
    10321 ttcatgcatg cctgtcttca gtcatacggg gagagcatct gtgatacagt ccatacattt
    10381 gttgcggacg tcgctcctga gtcactgact ggtctgcaaa cagtttatct gtacacggca
    10441 gggcttctgt gtgatatcgt ttttcatatc gagggggggc ctccagagat gaatggaata
    10501 ataatatttc ctgatgatat aataccttag agaatttctg tgtatgctat tgtgaacata
    10561 tt
//
LOCUS       DAZGCS010000141        10409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGCS010000141 DAZGCS010000000
VERSION     DAZGCS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.143"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..621
                     /gene="ssuE"
                     /locus_tag="ACHK01_004315"
     CDS             46..621
                     /gene="ssuE"
                     /locus_tag="ACHK01_004315"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO8099152.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            614..1573
                     /gene="ssuA"
                     /locus_tag="ACHK01_004316"
     CDS             614..1573
                     /gene="ssuA"
                     /locus_tag="ACHK01_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO8099153.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADTRAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1570..2715
                     /gene="ssuD"
                     /locus_tag="ACHK01_004317"
     CDS             1570..2715
                     /gene="ssuD"
                     /locus_tag="ACHK01_004317"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO8099154.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2726..3517
                     /gene="ssuC"
                     /locus_tag="ACHK01_004318"
     CDS             2726..3517
                     /gene="ssuC"
                     /locus_tag="ACHK01_004318"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO8099155.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3514..4281
                     /gene="ssuB"
                     /locus_tag="ACHK01_004319"
     CDS             3514..4281
                     /gene="ssuB"
                     /locus_tag="ACHK01_004319"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8099156.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4488..7100)
                     /gene="pepN"
                     /locus_tag="ACHK01_004320"
     CDS             complement(4488..7100)
                     /gene="pepN"
                     /locus_tag="ACHK01_004320"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO8099157.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7366..8568
                     /gene="pncB"
                     /locus_tag="ACHK01_004321"
     CDS             7366..8568
                     /gene="pncB"
                     /locus_tag="ACHK01_004321"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO8099158.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8737..10137
                     /gene="asnS"
                     /locus_tag="ACHK01_004322"
     CDS             8737..10137
                     /gene="asnS"
                     /locus_tag="ACHK01_004322"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO8099159.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2352 a         2691 c         2800 g         2566 t
ORIGIN      
        1 tatcaatgtt aacaaaaaaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc
       61 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
      121 aaactaaatg gcctcgatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
      181 gatctgcttt atgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      241 caggctgatg gactgatagt cgctacacct gtgtataaag ccgcctattc cggcgcgctg
      301 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      361 gcgacgggcg gtaccgtggc ccatctgctg gcggtcgatt atgcccttaa accagtttta
      421 agcgcactga aagctcagga gatcctgcac ggcgtgtttg ccgatgactc acaagtaatt
      481 gattaccatc acagacccca gttcacgcca aatctgcaaa cccgtcttga taccgcgcta
      541 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      601 cgaggtaatg cccatgcgta acatcattaa actggcgctg gtgggattgc ttagcgtctc
      661 tacgattgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      721 cagtattggt atggtactgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      781 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      841 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      901 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gctgaagtga ttctggtggc
      961 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
     1021 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaagtttac
     1081 cgatatccaa cccacttacc tgacgcccgc tgatacccgc gccgcgttcc agcaaggtaa
     1141 cgttgacgcc tgggctatct gggatcccta ctactctgct gcattattac agggcggcgt
     1201 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1261 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctcgcaacct ttagtgaggc
     1321 cgatgcgtta acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg
     1381 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1441 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaatcg
     1501 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
     1561 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat
     1621 cttggaacgg aagaaggttc acgcccggtt gatcacggct atctgcaaca aattgcgcaa
     1681 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1741 gcgtggctgg tggcggcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1801 ctgcgcccca gcgtaacctc acctaccgtt gccgctcgcc aggccgccac gcttgaccgt
     1861 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca
     1921 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1981 gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg
     2041 cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc
     2101 ggatcgtcag acgtcgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg
     2161 ggcgaaccgc cagaactagt aaaagagaag atcgaacaag tgcgggcgaa agctgccgcg
     2221 catggacgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac
     2281 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgaaac tatcgccaaa
     2341 gcacaggccg cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgttacat
     2401 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2461 cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgctgc gcgaatcaac
     2521 gaatacgccg cgcttggcat cgacagcttt gtgctttcgg gctatccgca tctggaagaa
     2581 gcgtatcggg tcggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2641 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcga atgattttat cccccgtaaa
     2701 gtcgcgcaaa gctaaggaag aaataatggc aacgccagtg aagaagtggt tattgcgcgt
     2761 tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct cctcagttgg
     2821 ctggctgtcg acgcgtattt tgccctcacc ggaaggggtg gtgacggcgt tctggacgct
     2881 ctccgccagc ggcgaactgt ggcagcatct ggcgatcagt tcctggcggg cgctgattgg
     2941 cttttcaatt ggcggatcgc tggggttgat tttgggactg attagcgggc tatctcgctg
     3001 gggagaacgg ctgctggata cctcaattca gatgttgcgc aacgtaccgc atctggcgct
     3061 gatcccgctg gtgattttgt ggtttggcat tgatgaatcc gcaaaaatct ttctggtggc
     3121 gctcggcacg ctattcccca tttatatcaa cacctggcat ggcatccgta atatcgatcg
     3181 cggtctggtg gaaatggcgc gtagctatgg attatccggc ataccgctgt ttatccatgt
     3241 gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg cgttttgcgt tgggcctgat
     3301 gtggctgacg ctgattgttg ccgaaaccat ttctgccaat tcaggcattg gttatctggc
     3361 gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta gtcgctatta ttctttacgc
     3421 cctgctcggc aaacttgctg acgtcagcgc gcagctgtta gagcgcctct ggttgcgctg
     3481 gaacccggct tatcatttga aggaggccac ggtatgaata ctgctcgtct gaaccagggc
     3541 acaccattgt tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt cctgaaccaa
     3601 ctggatttac atattccggc aggtcagttt gtggcggtgg tgggccgcag cggtggtggc
     3661 aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc caaccgcagg cgatgtgtta
     3721 gcgggcacca caccgctggc tgagattcag gaagacacgc gaatgatgtt tcaggatgcg
     3781 cgtctgctgc cgtggaaatc ggtgattgat aacgttgggt taggccttaa aggccagtgg
     3841 cgcgatgccg cgcgtcgggc gttggctgca gtaggactgg agaatcgcgc cggggaatgg
     3901 cctgccgcac tttctggcgg gcagaaacag cgtgtggcgc tggcaagagc gttgattcat
     3961 cgaccgggtt tattgttgct tgatgaaccg ctcggggcgc tggacgcctt aacgcgactc
     4021 gagatgcagg atttgattgt gtcactttgg cagcagcacg gctttaccgt actgttggtg
     4081 acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat tgaagaggga
     4141 aaaattggtc tggatttgac agtggatatc ccccgtccgc gtcgattagg gtcggtgcga
     4201 ttagcggagc tggaagcgga agtattgcaa cgggtaatgc ggcgtgggca ttctgaacaa
     4261 ctgattcggc gtcatggtta atatgccgga tgtggcgcac aaaaccagca ttttcaacgg
     4321 actagtaggc ctgataagac acgtcaagca tcgcatcagg cagttgggca cggctgccgg
     4381 atgcggcgtg aacgccttat ccagcctaca aaaccagcaa tttcaacgga gttgtaggcc
     4441 tgataagacg cgtcaagcgt cgcatcaggc attttggctc aaaagtatca agccagtgct
     4501 ttggtgatct tctcgtacag atcgccagag agattttcca gccctttcaa ctgttccagc
     4561 gccgcgcgca ttttctcctg acgtttggca tcataacgtt tcaggcgaat cagcggttca
     4621 atcagacgtg aagctacctg cgggttacgg ctgttgaggt cggtaagcat ttccaccagg
     4681 aactggtaac cgctgccatc ttcggcatgg aacgctgccg ggttgctgcc cgcaaacgca
     4741 ccaatcaacg aacgaatacg gttcgggttg ctcatggtaa atgagcgatg ctgcaacaga
     4801 ccacgcaccg tctccagcac attcgccgcc gggctggtgg cttgcaggat aaaccattta
     4861 tccatcacca gaccgtcctg atgccactta tcgtcgtact cctgcatcag cgcgtcacgg
     4921 caaggcagct gtgcagcaac cgccgcagaa agcgctgcca gcgcatcggt catattgttt
     4981 gcttcatggt attgcttgct caccagcacg tcagccagat gcgtttcacc aaaagcaagg
     5041 aagcgcaggc aggcattacg cagagtgcgt tttgcaatat cttcatgctc aacacggtac
     5101 tcgctctggt aattcgcgtt gtaaatagcc agcagctcat ccgccagttc agtcgccaga
     5161 gtacgagtga gtgcttcgcg tacttcggca atagcaatcg ggtcgatgat atcgaacagt
     5221 tcagccattt cattgacaga aggcagcgtc aggatttctg ccgccagcgc cggatcaatc
     5281 ttctcatcga gcagcaccgc gcggaaagcg tcagccacat gtaccggcag agacagcggc
     5341 tgcccttgct gatgacgggc gacgttcagc ttgatgtagg ttgccagcaa actttgcgcc
     5401 gcatcccaac gggagaaatc attacgcgca tgacgcatca ggaaggtcag ttgctgatcg
     5461 ctccacttat attccagttt cactggcgca gagaattcgc acagcagcgc aggcaccggc
     5521 tggaagtaga cattatcaaa aacaaaggtc tgttccgcct gggtgacgtt cagcacggaa
     5581 ttcaccggat gaccgccttt ctgcaacggg atcactttgc cttcgttgtc atacaattcg
     5641 atggcaaatg gaatatgcag cggctgtttt tctgcctgat ccggcgtggc tggcgtgcgc
     5701 tggctgatgg tcagggtgta ctgctcggtt tccggattgt agtcgtcttt gacggtcaca
     5761 atcggcgtgc cggactggct gtaccaacgg cggaaatggg agagatcgac attcgacgca
     5821 tcttccatcg cctgcacaaa gtcgtcgcag gttgccgcgc tgccatcatg gcgctcgaaa
     5881 tatagttgca tccctttctg gaagttttct tcgcccagca gggtgtggat catgcgaatc
     5941 acttccgcgc ccttctcgta aacggtcagg gtgtagaagt tgttcatctc aatgaccata
     6001 tccgggcgga tcgggtgcgc catcgggctg gcgtcttccg caaactgcaa tccgcgcatg
     6061 gtgcgcacat tattgatacg gtttaccgcg cgggaaccaa ggtcagagct gaattcctga
     6121 tcgcggaaga cggttaaacc ttctttcagg ctgagctgga accagtcgcg gcaggtcact
     6181 cggttaccgg tccagttatg gaaatattca tggccgataa cgcgttcaat atcgagataa
     6241 tctttgtcgg tggcggtgtc ggtgcgggcc aacacatatt tggagttaaa gatattcaga
     6301 cccttattct ccattgcgcc catattgaag aaatccaccg cgacgatcat atagatgtcg
     6361 aggtcatact ccaggccaaa gcgttcttca tcccatttca tggagttttt cagcgaggtc
     6421 atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt acagctccag tgctacttcg
     6481 cgaccagaac gcgtggtaaa ggtatcgcgc agtacatcaa agtcgcctgc caccagcgca
     6541 aacaggtagc acggtttcgg gaacgggtcc tgccactgta cccaatggcg tccgttttcc
     6601 agttcgcctt gcgcaacgcg gttaccattg gaaagcagga agggatattt gattttatcg
     6661 gcaataattt tggtggtaaa acgcgccagc acgtccgggc ggtcgagata atacgtaata
     6721 tggcggaaac cttcggcttc acactgggtg caaagcgcat cgcctgactg ataaagccct
     6781 tccagcgccg tattcgccgc cgggcttatt tcattaatga tcttaagcgt aaaacgctcc
     6841 ggcaaattac tgatgaccag tgcgccctct tcttctttcc aggcggtcca cggctcatca
     6901 ttaatatgaa cagaaaccag tttgaggtct tcgccgttga gacgaagcgg agcatctgat
     6961 gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg tcttttgcgc gtcgaggtca
     7021 aaggtcaagt caatatcagt aatctggtaa tccggcgcac gataatcgtg acggtatttg
     7081 gcttgtggct gttgagtcat aaataacctt tagcatcttt tatagagtct ggtgttcagt
     7141 ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat cttttcaggt acaaacgcct
     7201 ttattgctac atttttataa catacaccgc gtaatgccat cgaccagaaa ggtggcatat
     7261 ggtgtgatcg gggttcaata aattgcgaaa caaggtatac tccagcagtt cctgaagatg
     7321 tttattgtac taaacgctcc tgtacgagga cgctactgcg cacctatgac acaattcgct
     7381 tctcctgttc tgcactcgtt gctggataca gatgcttata agttgcatat gcagcaagcc
     7441 gtgtttcatc actattacga tgtgcatgtc gcggcggagt ttcgttgccg aggtgacgat
     7501 ctgctgggta tttatgccga tgctattcgt gaacagattc aggcgatgca gcacctgcgc
     7561 ctgcaggatg atgaatatca gtggctctct gctctgcctt tctttaaggc cgactatctt
     7621 aactggttac gcgagttccg ctttaacccg gaacaagtca ccgtatccaa cgataatggc
     7681 aagctggata ttcgtttaag cggcccgtgg cgtgaagtca tcctctggga agttcctttg
     7741 ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac cgcaggccga cgttgcgcaa
     7801 gccctcgaca cgctggaaag caaattagtc gacttctcgg cgttaaccgc cggccttgat
     7861 atgtcgcgct tccatcttat ggattttggc acccgtcgcc gtttttctcg cgaagtacaa
     7921 gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg tgggcaccag caactacgat
     7981 ctggcgcgtc ggctttccct cacgccgatg ggaacacagg cacacgaatg gttccaggca
     8041 catcagcaaa tcagcccgga tctagccaac agccagcgag ctgcacttgc tgcctggctg
     8101 gaagaatatc ccgaccaact tggcattgca ttaaccgact gcatcactat ggatgctttc
     8161 ctgcgtgatt tcggtgtcga gttcgccagt cgctatcagg gcctgcgtca tgactctggc
     8221 gacccggttg aatggggtga aaaagccatt gtacattatg aaaagctggg aattgatcca
     8281 cagagtaaaa cgctggtttt ctctgacaat ctggatttac gcaaagcggt tgagctatac
     8341 cgccacttct cttcccgcgt gcaattaagt tttggtattg ggactcgcct gacctgcgat
     8401 atcccccagg taaaacccct gaatattgtg attaagttgg ttgagtgtaa cggtaaaccg
     8461 gtggcgaaac tttctgacag ccctggcaaa actatctgcc acgataaagc gtttgttcgg
     8521 gcgctgcgca aagcgttcga ccttccgcat attaaaaaag ccagttaata tcatcaggga
     8581 gctaatcggc tccctttttt tacctttaat tccgaaatct ttcgctgcat ttgcgaattc
     8641 tgcttgtctg attgcagatg ccaggtaaca taggtatccc cccatttacg ggtggacaag
     8701 tgtttatttt ttccgactat taacagagag aatattatga gcgttgtgcc tgtagccgac
     8761 gtactccagg gccgcgtagc cgttgacagc gaagtcaccg tacgcggatg ggtacgtacc
     8821 cgccgagatt caaaagctgg catctccttc ctcgccgttt atgacggttc ctgctttgat
     8881 cctgtacagg ctgtcatcaa taattctctg cccaattaca atgaagacgt cctgcgtctg
     8941 accaccggct gctcggtcat tgtgacgggt aaagtcgtgg cgtcgccggg ccaggggcaa
     9001 caatttgaaa ttcaggccag caaagttgaa gttgctggtt gggttgaaga tccagacact
     9061 tacccgatgg cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc tcacctgcgt
     9121 ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc atacgctggc gcaggcgctg
     9181 catcgcttct ttaacgagca gggattcttc tgggtttcaa cgccactgat taccgcatct
     9241 gataccgaag gtgcaggcga aatgttccgc gtttctacgc tggatctgga aaacctgccg
     9301 cgtaacgatc agggcaaagt ggatttcgac aaagacttct ttggtaaaga gtctttcctg
     9361 accgtatctg gccagttgaa cggcgaaacc tacgcttgcg cattatccaa aatttatacc
     9421 ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc ggaattctgg
     9481 atgctggagc cggaagtggc gtttgctaac ctgaacgata tcgcgggtct ggctgaagcc
     9541 atgctgaaat atgtcttcaa agcggttctc gaagaacgcg cagacgacat gaaattcttc
     9601 gctgaacgcg tagataaaga tgccgtttca cgtctggaac gcttcattga ggccgatttc
     9661 gcgcaggtgg attacaccga cgcagtgacc attctcgaaa actgcggcag gaagtttgaa
     9721 aacccggttt actggggcgt cgatctctct tctgagcatg agcgttatct ggcggaagaa
     9781 cactttaaag caccggtagt ggttaaaaac tatccgaaag atattaaagc gttctatatg
     9841 cgcctcaacg aagacggtaa aaccgttgcg gctatggacg ttctggctcc gggcatcggt
     9901 gagatcattg gtggctccca gcgtgaagaa cgtctggacg ttctggacga gcgtatgctg
     9961 gaaatgggcc tgaataaaga agattactgg tggtatcgcg atctgcgtcg ctacggtact
    10021 gttccgcatt caggtttcgg tcttggtttt gaacgcctga ttgcttacgt aactggcgtg
    10081 caaaacgtgc gtgatgtgat tccgttccca cgtactccgc gcaacgccag cttctgattt
    10141 tcttcctcaa agagccagcg cctgctggct cttttttatc ctttctgtca agttatctgt
    10201 ttgttaagtc aagcaatcta tttgcaaccc cgccataacg atcatcctgt tacggaatat
    10261 tacattgcaa catttacgca caaaaaataa cccgcattct tattgcggat tagttttttc
    10321 ttagctgata gcacaatttt catactattt tttggcattc tggatgtctg aaagaggatt
    10381 ttgtgccagg tcgataaagt ttccatcag
//
LOCUS       DAZGCS010000142        10370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGCS010000142 DAZGCS010000000
VERSION     DAZGCS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.144"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            212..1894
                     /gene="ydeN"
                     /locus_tag="ACHK01_004323"
     CDS             212..1894
                     /gene="ydeN"
                     /locus_tag="ACHK01_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8099160.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1946..3103
                     /gene="ydeM"
                     /locus_tag="ACHK01_004324"
     CDS             1946..3103
                     /gene="ydeM"
                     /locus_tag="ACHK01_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8099161.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3069..3191)
                     /locus_tag="ACHK01_004325"
     CDS             complement(3069..3191)
                     /locus_tag="ACHK01_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099162.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3394..5079
                     /gene="yddA"
                     /locus_tag="ACHK01_004326"
     CDS             3394..5079
                     /gene="yddA"
                     /locus_tag="ACHK01_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO8099163.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5117..7489
                     /gene="yddB"
                     /locus_tag="ACHK01_004327"
     CDS             5117..7489
                     /gene="yddB"
                     /locus_tag="ACHK01_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO8099164.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7546..10329
                     /gene="pqqL"
                     /locus_tag="ACHK01_004328"
     CDS             7546..10329
                     /gene="pqqL"
                     /locus_tag="ACHK01_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8099165.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMFETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRTT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     YPVNTYTGVLLPK"
BASE COUNT         3046 a         2286 c         2271 g         2767 t
ORIGIN      
        1 tgcaataatt tattaaatat caatagatta gttgtttatc ggaaagaaat tacttagtag
       61 aacagaaagt aatgtcaacg ctttatggac tgttatttcc ctttttttag ctaaatctgc
      121 tatctcttta tgtgactaac ttcacttaca cccacttatt tctcttcgta aaattacttc
      181 ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt
      241 aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg ctcatgcggc
      301 agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact ttacgccgac
      361 tgaatacagt accaaaggaa agccaaatat tatcgtacta accatggatg atcttggtta
      421 tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa atcgtgaagt
      481 tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcacaaa aatcaacgcc
      541 gacgctcctt tcattaatgg atgaaggcgt acggtttact aacggctatg tggcacacgg
      601 tgtttccggc ccctcccgtg ccgcaataat gaccggtcga gctcccgccc gctttggtgt
      661 ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct tgcctgaatt
      721 attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt caaaaatcag
      781 taatgtgccg gtaccggaag ataaacaaac gcgtgactat catgacaact tcaccacatt
      841 ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg gattccacgc
      901 tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac gtgtccccgc
      961 aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa
     1021 aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc tgccaaatga
     1081 taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa cagcagataa
     1141 ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa
     1201 gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg gtgcggttat
     1261 cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga cctatcctgg
     1321 cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga
     1381 caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg atatcagcat
     1441 tccaaaagac cttaagctgg atggcgtttc cttgctgccc tggttgcaag ataagaaaca
     1501 aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt ttgacgagga
     1561 aaatattcca ttctgggata attaccacaa atttgttcgc catcagtcag acgattaccc
     1621 gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa ataacgatta
     1681 ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga cggatctaca
     1741 gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt
     1801 aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg agaagtttaa
     1861 caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg cggaattttc
     1921 cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca gttttcaatg
     1981 taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa
     2041 atggatggat gacagtactc tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa
     2101 tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg attttttccg
     2161 taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta atgcattaca
     2221 aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac atgaatttct
     2281 ggttggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac gcagtaattc
     2341 aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat catatcaagt
     2401 tgagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc ttgaggttta
     2461 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg
     2521 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
     2581 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa
     2641 cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa
     2701 tgggcataca agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
     2761 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
     2821 taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
     2881 agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgtc ctaagcatcg
     2941 tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata aaatcctttt
     3001 ttcaaccatg gtaccttata tgaacgccat ggtggagtta gctaagaaca gagtaccgct
     3061 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct ctcttttaac
     3121 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcggg
     3181 tgtttcgtca tcatcatctt cccttgcgaa aatcctggct tacgtggtat gcgtcagggc
     3241 attagcaata cccttgatta tcaatagcat aaccataacc actgaaaatg aaatattcct
     3301 ttatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc attgccatag
     3361 ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat tacgttttgt
     3421 atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa gaataataaa
     3481 acttcagtgt tgttaatcat tattatcctc gccatgatcc ttggcgtggt taaaattcag
     3541 gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa agaaaccgat
     3601 aagctttggc agcttgtcct ttggttccct gccctgctgg ggatctttgt attaatatcc
     3661 gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg gttaacagat
     3721 tactacctta atcggtggtt tgcagataaa aactactact tcacgcagat ctacggcgag
     3781 cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt actgatcagc
     3841 aaaacgctca gcctttcatt tggcttcatc cagtcactta gcatgctgat cacctttacc
     3901 gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac agaatggaat
     3961 atccaggggt atatggtcta taccgttgtg ctcattgtga tcggtggaac tttatttacc
     4021 cataaggttg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca
     4081 tttcgaacaa atcttgtgca gcataataag caggcagaac tgattgcgct aagcaatgct
     4141 gagtctctac aacgccagga gttgagcgac aattttcata ccattaaaga gaactggcat
     4201 cgtttaatga atcgtcagcg gtggcttgat tactggcaga atatttattc gcgctcgctt
     4261 agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat caatctgggc
     4321 ggactgatga aatcgcgcca ggcatttatg ctggtatcga acaatttaag ctggtttatt
     4381 tataaatatg acgaacttgc tgagctggct gcggttatcg atcgcttgta tgagttccat
     4441 caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc ggtacaagtg
     4501 gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt gaactttcat
     4561 gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa aaccacactg
     4621 cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc tcctgctgac
     4681 agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga gattatttgt
     4741 aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca tcaggttggt
     4801 ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat tctttccagc
     4861 ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa atggatattt
     4921 cttgacgaaa ctacctctca tcttgaggaa caagaggcta tccgcttact gcgtttagtg
     4981 cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg tgtctggaac
     5041 ctggcagatg atatttgtga cattagcgcg gttttataaa aacaataaaa gccctgctgt
     5101 acggagatat aaataaatga agcgagttct tattcctggc gtcattttat gtggcgctga
     5161 tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat gtttttgaag agatgacggt
     5221 ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg aaagcatcga
     5281 gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc ctgcggtacg
     5341 catggattca acgcaaagta cctcgttgaa ccagggagat attcgcccgg agaaaatctc
     5401 tattcacggt gcgtcgccct atcagaatgc ctatttgatt gacggtatta gtgccactaa
     5461 taacttgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta gcgggatgtc
     5521 acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg acagttttgt
     5581 gccggttgaa ttcggtcgct tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa
     5641 cgctgatgat agcagcgtga aactgggtta tcgcactacg cgttcggact ggttaacatc
     5701 gcatatcgat gagaataaca agagcgcatt taatcaaggc tcttcaggaa gtacttatta
     5761 ctccccagat tttaaaaaga acttttatac cttgtcgttt aatcaggaac tcgctgataa
     5821 ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg cggattatgt
     5881 ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg ataccgcatt
     5941 gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct taaaatatac
     6001 cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg aaatgggtaa
     6061 taaatcctat ggtctggcat gggatatgga tacgcagctc gcatgggcca aactacgtac
     6121 caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca tctggtacac
     6181 cgaactttca tgtacatatg gtgatattac agggcgttgc acccgtggcg gattaggaca
     6241 catttcccag gctgtagata attacacctt caaaacacgc ctggactggc aaaaattcgc
     6301 cgtgggtaat gtttcgcatc aaccctactt cggcgcggaa tacatctatt ccgatgcgtg
     6361 gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgccggaa agaaaactaa
     6421 ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca cgctgtatat
     6481 ggcggatcgc attagctggc gtaatgtgtc attaatgccc ggcgtgcggt atgactatga
     6541 caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg atatttttgc
     6601 taatcaaacc tcaatgatta cagcaggtta taaccgttac tatggcggga atattcttga
     6661 tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta ataaaactct
     6721 gacgcgttat caggatttga aaacgcctta taacgatgaa ctggcaatgg gattgcagca
     6781 gaaaatcggt aagaacgtta ttgcgcgtgc aaactatgtt taccgtgaag cgcatgatca
     6841 aataagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg aatataacaa
     6901 cgatggcaaa accaaaacgc attcgttcag cctcagtttt gaactggccg aacccctgca
     6961 tatccgccag gtagatatta acccacaaat tgtctttagc tatatcaaga gcaagggcaa
     7021 cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg tggtttataa
     7081 cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc cattaaagat
     7141 ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga atacgctggc
     7201 ctggcaagaa gcgcgtaaag ctcgcattat cctgggtaag acaaatgcgc aatacatcag
     7261 cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc tgacctggga
     7321 cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga tcagtgccga
     7381 tattctcaat gtattggata gcaaaaccgc tgttgataca acgaataccg gtgtggcgac
     7441 ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat agttcggatt
     7501 ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag aaacctctgt
     7561 ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc cgccgcctta
     7621 ccgcaggatg aaaagttaat taccgggcaa ctgggcaatg gcttgcgata tatgatttat
     7681 ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac cggttcattg
     7741 caggaagaag acaatgagcg cggcgtagct cattttgtag aacatatgat gtttaacggc
     7801 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
     7861 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
     7921 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
     7981 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
     8041 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
     8101 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacaccg
     8161 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
     8221 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
     8281 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
     8341 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
     8401 ccaatggtac aagtgaacga tgagcaaagc tttatcgaac aagctgaatg gagcatgtta
     8461 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
     8521 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
     8581 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
     8641 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
     8701 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     8761 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     8821 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     8881 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     8941 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     9001 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     9061 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     9121 gccagggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tactgccgta
     9181 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     9241 aaagcagtaa gcggaagcgg cgttggtgag ctctcctctt ccagcctgaa acgctggagt
     9301 gcggaaaatt cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     9361 agcgcgcgga ctaataaccc tgaacctggt tttcagttga ttaaccagcg aatcacccac
     9421 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     9481 acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtttgagac gcgctatgct
     9541 gatgaccgca cgaaattact gcaagaaaat cagattgtac agtttactgc cgcagatgcg
     9601 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     9661 aatgtctcag aagacaaact cgtggcgtta attacgcgtt acttaggatc aatcaaacac
     9721 tctgattcgc cattagccgc aggtaaacca ttaactcgca cgacggacaa cgcatcggtt
     9781 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     9841 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     9901 aaagatctac gtgttaatat tcgtgaacag gcatctggag catacagcgt ttcttctcgc
     9961 ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac ttgtcaacca
    10021 gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg tctggctaaa
    10081 gggatcagtg agcaagaact gaatgaatac cagcaaaacg ttcagcgcag cctcgatatc
    10141 caacagcgta gcgttcaaca attagcgaac actattgtaa atagtcttat tcaatatgac
    10201 gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt agagaatgtt
    10261 aacactgccg ttaaacaata tctttcttat ccggtaaata cttataccgg agtattattg
    10321 ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
//
LOCUS       DAZGCS010000143        10310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGCS010000143 DAZGCS010000000
VERSION     DAZGCS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.145"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..123)
                     /locus_tag="ACHK01_004329"
     CDS             complement(<1..123)
                     /locus_tag="ACHK01_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099166.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHSLG"
     gene            320..1056
                     /locus_tag="ACHK01_004330"
                     /pseudo
     CDS             320..1056
                     /locus_tag="ACHK01_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1235..1531
                     /locus_tag="ACHK01_004331"
     CDS             1235..1531
                     /locus_tag="ACHK01_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099167.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1839..1955
                     /locus_tag="ACHK01_004332"
     CDS             1839..1955
                     /locus_tag="ACHK01_004332"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099168.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2174..3607
                     /locus_tag="ACHK01_004333"
     CDS             2174..3607
                     /locus_tag="ACHK01_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8099169.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4428..4991
                     /locus_tag="ACHK01_004334"
     CDS             4428..4991
                     /locus_tag="ACHK01_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8099170.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            5146..7506
                     /locus_tag="ACHK01_004335"
     CDS             5146..7506
                     /locus_tag="ACHK01_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO8099171.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7668..7844)
                     /locus_tag="ACHK01_004336"
     CDS             complement(7668..7844)
                     /locus_tag="ACHK01_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099172.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7982..8224)
                     /locus_tag="ACHK01_004337"
                     /pseudo
     CDS             complement(7982..8224)
                     /locus_tag="ACHK01_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8263..9795)
                     /locus_tag="ACHK01_004338"
     CDS             complement(8263..9795)
                     /locus_tag="ACHK01_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO8099173.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQTPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTGRLWAYVRDDRNAGS
                     ALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARR
                     KIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWL
                     REKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFL
                     FFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALP
                     AE"
     gene            complement(9845..10192)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004339"
     CDS             complement(9845..10192)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8099174.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT         2717 a         2244 c         2523 g         2826 t
ORIGIN      
        1 gcccagtgaa tgttgatgtg aggaaaaatt tttaatattt cggatgtcac ggctattctg
       61 gtttttagcc agaacaccaa aacatgaaaa gacatcatac attatagtga taaaattata
      121 cataacattc tccttttatt gcactatata aaattttaac attatatcat tttgcttttc
      181 tgttaactgg ttttcatttt tgaatcaata ttttctgaac agggtaatag tacacgtatt
      241 aacagattat gtcagaaggc cccggtgatg ccttctgacg atagattaat gttgtattct
      301 attggtatct ggagaccata tggcaacagt tacagtatat tctccccgct gtaattccta
      361 taaagtgtac cgttatgatc acagcacctc agatcatgag cgtttccagt gctaatcctg
      421 caaacgtgta tttcaactga attacatcta cgaaacccga aaaccaggca ttaaagagca
      481 aattgttgaa atggttcata acggagccgg ggttcgcgat agctccagaa cgctgaaggt
      541 ggatatcaac accgtcattc tcactttaaa aaacgctcac cacgtaaagt aaccatatct
      601 ccagccgcca gtgtcgatgt ggcacttatc tgtgaacttg atgaacaatg gagttttgtc
      661 gaaaacaaag ctcgtcagca gtggcactgg tacgcgtata agaccaaagc tgacggtgtg
      721 ctggcttaca cttttggtcc tcgcactgat gaaacatgcc gtgagttgcc ggaattcctg
      781 aagccattct ccgcaggcat gataaccaga gacaaccgga gcagttatac cagagaaatg
      841 ccacaggata agcatcttgt cggcaagata tttacccggc gcattgagcg caacaatctg
      901 acacttcgta ctcacatcaa acgacctgcc cggaaaacga tttgcttttt acgctccctg
      961 gaaattcacg aaaagccatt ggttcattta tcgaaaacac atgtactact gacaggagtc
     1021 attaccaggg cctcttttgc tgtttttttg ccatgaatga taatgagaat cgacagcgat
     1081 aagggaaaag taaaataaca ctaatgctgt cgttttctat catattgatt tttaattatt
     1141 tattgtcttt tttctcaacc cattttttta cgcagacaaa tttacccggg gaatacaaac
     1201 tgtaacgaca gttcaccaca ctatggggga taaaatgcca gatcaatccg gacactgcag
     1261 gactccttca cgcatcttac ggatgcctga actgagccaa ttgttaggta tatctcgttc
     1321 gacaatttac gaaaaaatga atccgttatc gaaatactac gatgcaacct tccccaggcc
     1381 ggtacgtctt ggtagtggtt ctgtgggctg gcgttcatca gctattgacg aatggcttac
     1441 tttacatact gtacctgcca gatcagcagt caaaggtgtt aatgacgaag tgagacatac
     1501 gctttcttca gatatgacca tgagcagata agtttttgtc atcacgtgat cacctcagga
     1561 ggaaaaagaa gcataagaac aatttagcca gaagtttaat tgataaacag aatttccagc
     1621 attcattgtt gaaagagcag ctattgtcgc tattgtagcg gtaattgctg ctataaccga
     1681 aacgtattac gaaaaaggaa gcatttatgc cttacatcaa accggatatg tcgtattcag
     1741 tatatgcatg accatcaatg atagggaagg caataattaa agctcaatac aataaacaag
     1801 tacctctgac tctggttgca atgtcagtat taacagcgat gtcaattgcc tgtcagaatc
     1861 aggttgacgt atgcagtccc ggaaatttac gtggggccgt taatatttac actatggttc
     1921 tggagaaagg gaaaagaccg ggggaaaggt attagtgaaa taatttccgg aggtattaag
     1981 aatgagaaat atgaatgata tttcgtggtg aataatacag tcttcactga attaacctta
     2041 tcggtttatt aaacatcaga tctacaaatg ctctgtattt ctcagagcat caggtctgaa
     2101 tagcagctat tttcgctatt gtagcgggat ttgctgctat tttactaacc cattaatgaa
     2161 aagagaggct tttatgtgct tacttgcacc agaaaatcct tatccgatat atgcactacc
     2221 accgctggtg agaaatgcaa taattgaaac tcaaaaaaat acacaggcac ctttggctat
     2281 ggtggcaaca tccgcattaa cagcgatctc aattgcctgt cagaatcaga ttgacgtgtg
     2341 cagacctgga aatttacatg ggccagttaa tctttactct ctgattctgg ctgattccgg
     2401 tgaaaggaaa acgaccgtgg ataaagtgtt tatgaaagca ttttatctca gggatgaagc
     2461 cctggcggat gaatacgcga aactggttga gaattacagt acagaaaagg aaatatggga
     2521 gcaaaaacaa aaagcgctgg aatcaaaatt tcataaagag attcgtgccg gtaaagatta
     2581 caaggcaaca gaatcagagc ttgaaacgca tctgaataaa cctcctgttc cgccgcagat
     2641 acgtcgaaca atttttaatg agacaacgat agagggaatg ttaaaatatt actccgatag
     2701 caatcgctct tttgctcttg tatccagtga agggggggta atttttgaca gccgggccat
     2761 gagtaaactg ggaattatta acactttgtg ggatggaggt tctcttttca tcgacaggaa
     2821 atcatctccc ggaattaatt tgaaggagcc aagactgacg atatcggtga tgattcagcc
     2881 tgatgtttac cacaaaggtt tttgtacgcg aaaaaaagaa attgtgaaaa cgtcaggaca
     2941 tcatgcaagg tttttgatgt gtcaaccaac atcaacgcag gggacaagga taataaccgg
     3001 agataattat tcatcacagt atcaggattt gtttgaagag aggatcaatg agcttattga
     3061 tgaaagtctg gcgatgagtg gtgaacgacg ttgccttcac ttttctcctc aggctgccag
     3121 aatctggacg gattattaca atgatgtgga atcgaagctg gggggattgg ggcctttaag
     3181 acattgccgg gaatatgctg ctaaaaatgc agagtatatg gcaagactgg caggacttat
     3241 ttaccattcg agcggtgaag agggggagat ttccccttac actgcagaaa tggcgagaga
     3301 attagcaata tggtacggta atgagtatgt gcggttgtct aatccgttaa cttttgacaa
     3361 ctctgccctg accgtacctg tgcgactcat tccagaggag cttgaacttt tcaactggat
     3421 aaaaagctat tgtattgaga aggggatcct ctgtatgaaa aaaaatgaca ttttacagcg
     3481 tggtccgaac cgtttccgga aaaaggataa aatcaactgg ttactggatt tactgtatga
     3541 acaaaacaga gttgtaccgg ttattgaggg aaaaacgttg tgtgtcgcac ctaactttga
     3601 cctctgaacg acttaatcgt tgagtacata tagtctcctc tgctacttgc ggaggagact
     3661 aatgtcttta ccatgtcagc gttgtattag aaaactctgg tacaggtcag tcagtaaata
     3721 taactggtgt aaccactatt tactattgga tagagtgtta ctcaggatat tgtcggcttt
     3781 agtctgaggt gaggtggggc cttcattgtt ttcgtgttca ataaataatt taggttaatt
     3841 agtattatta gtggaagtta tgtgatctgt tatgttgtct tgtacgcact taacactggt
     3901 tacgtaccgg aacactatta gagtatgtta tggctctgtt acagtttata ggtatgaaaa
     3961 aatataatct ttcctgtatg aaataatatt cttatggtgt gtggggtatg gtctgtatac
     4021 catcattttt atggtgaagt taaatatctg tagaggcctc aagctgggtc gctgttttta
     4081 cttaatggta cagaagtatt aatagcggtg ttgcctgtgg ctacccgata ctggcttttg
     4141 tgaatgtcag attacttcct gtaagtgatt gaatggtact tccttaaact caattctatg
     4201 aggtgtgttt ttaccattgt aattcgtact ggtcctcagt ttaagtgagt cttactgtac
     4261 tctgaaatat tacctctttg gagtaatgag gtaatacgaa acagttttat caggttgtct
     4321 gtgctgctgg ttacctcgtt tattaatgcc gcggtacggc gtttctgtac tctcagtatt
     4381 tatgtaattc caccttcctg taacttttat aaagtaagga ctataaaatg tataatgcta
     4441 atcccaatta cgaaatggac ttcatgattc tgaaagacgt gaatgaacac atggaaggtc
     4501 tgtttcaacg tttttctaaa ctgttacctt tcagaattga ttttgcctac aggaaagaca
     4561 cgcccagttt tggtcattcc tgtaggcaca gtatgtgtat tgaaatgcac cgcttactta
     4621 gtgaaaccca aacgatgctg gcgggatatt actgggtaat ggaatatacg tcaaacaagg
     4681 gacttcatat tcattttatc ggctacctgg atgggcaacg tcacaacaaa tcttatcgga
     4741 tatccaggca actgggagat atctggaggc ggatcacgga aggggagggt tattttcacc
     4801 tgtgcagggc taaagacaaa tacccggtgc gtatcgatca cgtaattcac tatagtgata
     4861 aatctgcggt agacgactta cgttacgccc ttagctatct ggccaaacag gatcagaaag
     4921 aacacggtat tattctgagg cgtagcaggc ttcccgaaaa aagtaaccgg ggccgaccac
     4981 gacataactg aattacagct gtattaggta ttagaattaa gtttctgtaa gttgttggga
     5041 ggtgcttgag ttaataggca gaagagggcg gggcgctttg tcatcagtgt aggggacccg
     5101 aatttctgcc gtggtgcctc agtggatcgg atacaggtaa cgaatatggt tcataaatct
     5161 gacagtgatg aattagctgc attgagggca gaaaatgccc gtctggtctc attacttgaa
     5221 gctcatggga ttgaatggcg acgtaaaccg cagactcctg tgcagcgcgt ttctgtatta
     5281 tccaccgacg agaaggttgc attatttcgt cggttgtttc gtgggcgtga tgatgtatgg
     5341 gcacttagat gggaaagtaa aaccagcggc aaatcagggt actctcctgc ctgcgctaac
     5401 gaatggcagg cgagaatatg cggtaaaccc cggataaaat gtggggactg cgctcaccgt
     5461 cagttgattc ctgtatccga tctcgtcatc taccaccatc tggccggtac ccatactgtc
     5521 gggatgtatc cgttgctgga agatgattcc tgttattttc tggcagttga ttttgatgaa
     5581 gctgaatggc agaaggatgc atccgcattc atgcgatcct gtgatgagct gggtgtacct
     5641 gctgcgctgg aaatatcccg ttcacgtcag ggggcgcatg tctggatatt ttttgcctca
     5701 cgagtttcgg cccgcgaagc tcgccgtctg gggactgcta ttattagcta tacgtgtagt
     5761 cggacccgac agctgcgatt ggggtcttat gaccgattat ttcctaatca ggatactatg
     5821 ccaaaagggg gatttggtaa tctcatagcg ttacctctgc aaaaaagacc gcgtgaattg
     5881 gggggaagcg tttttgttga tatgaatctc cagccttatc ctgatcagtg ggcttttctt
     5941 gtatcggtga ccccgatgaa tgtgcaggat attgaaccga cgatattacg ggctacaggg
     6001 agtatccatc ctctggatgt gaattttatc aacgaagaag acctgggtac gccgtgggaa
     6061 gagaaaaaat catcaggaaa cagactgaat atttctattg cagaaccgct gaaaatcacg
     6121 ctggcaaacc agatctattt cgaaaaagcg caattacctc aggtgctgat taaccgactt
     6181 attcggctgg cagcatttcc gaaccctgag ttttataagg ctcaggcaat gcgtatgtca
     6241 gtctggaata agccccgtgt tataggttgt gcggagaatt acccgcaaca cattgcgttg
     6301 ccccggggat gtctggacag cgtattatct ttccttaggg acaacaatat tgctgcagaa
     6361 ttaatcgata aacgatttgc cgggacggaa tgtaatgccg tttttatggg aaacctcaga
     6421 gcggagcagg aagaggccgt ttcggcatta ctccgttatg acactggtgt gctttgtgcg
     6481 ccaacggctt ttggtaagac agttaccgca gcggcagtga ttgccaagcg gaaagtgaat
     6541 acactgatac tggtacaccg gactgaattg ctgaagcagt ggcaggagcg tctcgcggtg
     6601 tttctgcagg ccggtgacag tattggtatt atcgggggag gtaaacataa accctgtggc
     6661 aatattgata ttgcggtggt gcagtccata tccagacaag gagaagttga acctctggtc
     6721 aggaattatg ggcaaatcat tgtggatgag tgccatcata ttggcgcggt ttcattttct
     6781 gcgattctga aggaaacgaa tgccagatat ctgcttggcc tgacggcaac accaatccga
     6841 cgggatggtc tgcatcccat tatttttatg tactgtggtg ccattcgcca tacagcggtc
     6901 cgcccgaagg aaagcccaca taatctggag gtactgatcc gttcccgttt tacatctggt
     6961 catttaccat cggatgcgag aatccaggat attttcagag aaattgctct ggatcatgac
     7021 agaacggtgg cgatagctga agaagccatg aaagctttcg ggcaggggcg aaaagttctg
     7081 gtactgactg aacgtacaga tcatctggat gagatagcat cagtgatgaa ttcactgaaa
     7141 ttgtctccct ttattctcca tggtcgacta tcgaaaaaaa agcgtgcgat gctgatatcc
     7201 gggctgaatg ctcttcctcc cgattctcct cgaattttgt tgtcaacagg cagacttatt
     7261 ggtgagggat ttgaccaccc tccgctggat acgctgattc ttgccatgcc tgtgtcatgg
     7321 aaagggacat tacagcagta tgcagggcgt cttcacagag agcataccgg taaaagcgat
     7381 gtcaggatca ttgattttgt ggataccgcg tatcctgtgc tgctcagaat gtgggataaa
     7441 cgtcagcggg gttataaagc gatggggtac aggattatag ctgacggcga tgaatcagtg
     7501 atttaatttt aaggagtact ttaccctttt taaataattt gagctccccg ggttgttcgg
     7561 gcaacaaaga gtcccagtat acaggcttca ggaatatgtt gtagtatcag gcttttcatc
     7621 gctttaaaat gactaccggc agtgagaacg tcatcaaaaa taacaatcta gggcctgcac
     7681 ccttccagta attgttcatc cagttggtag tttgccataa tcgcttctgg taagggccgg
     7741 tttgttgcgg tatgagaggc tattgttgat acgttttgca caaccagttc ccagaaatcc
     7801 agccatggtt cgtaggtttg cgcaattcgc agaatttctg tcatacggtc atcgtaagcg
     7861 gcatctgtac ggcatttgga tggtggtacg ggaacaaatg tgtattcctc aagattaccg
     7921 atatttgata tgagcatctg agcaacatac tcaatagcag cctttttata gtggtaatca
     7981 ggctacgaaa gtacagcgtt ttataaattg tctcaggtga tattcgcagc gttttttgac
     8041 gtggttttgt tcgccttaac catcctgata tttgctctgg agaccatttc atctccagct
     8101 tttccagaac aagctttcgc aatggtaaat tttgatccag taagcacggt tttggccttt
     8161 tcgccattct gttggctcgg ttattagcat caacagcttt gtaatagcgt ctgccccgat
     8221 tacgcgtaag cgtacagcga gggccgtatt gacggggatg tgttattcag ctggcagtgc
     8281 tatgcgccac ggaagcagtt cgctgacccg gttgaccggc cagtctgcta tgacgccaag
     8341 cacatggcga aggtagcttt ctggatccac gtcattcagt ttgcacgtcc cgatcaggct
     8401 gtacagtagc gctccccgct caccaccatg atcagagccg aagaacagga agtttttacg
     8461 acccagactg accgcccgca gggcattttc agcgatgttg ttgtcgattt ccacccagcc
     8521 atcgttcgca tagtacgtca gtgccggcca ctggttaagt gcgtacgcga acgccttcgc
     8581 caactctgag tgtcgcgaca gggtcttcat cttttcacgc aaccagcttt ccagggattt
     8641 caacaacggt ttcgtttttc gctgacgttc agcaagccgc tgctctgccg gcattcccct
     8701 tatatccgcc tctatggcgt acaactgacc gatctgctcc agggcttctt ccgtcagtgc
     8761 tgacgggatg cggacgtgca catcgtggat ctttcggcgg gcatgagccc agcaggcagc
     8821 ttccgttatc ccaccattgc gatacagctc gttgaacccg gcgtacgcat ccgcttgcag
     8881 cacaccgctg aagcaggcaa gatgagtctg cggatggatg ccttttctgt ccgggctgta
     8941 agcgaaccac actgcaggtg ccaacgctga ccctgcattg cggtcatcac gaacatacgc
     9001 ccacaaccgc ccggtcttct tattacccgg cagcagtacc tggaccgggg tatcatcggc
     9061 atggagtttg ccgtcagtca tgacatagcc atgaagcgcc tcttccagcg gagacagcag
     9121 ccggcagcat gcatccaccc agcccgacag cagtgaacgc ctcagctcca caccttgccg
     9181 gccgtatatt tctgactggc gatacagcgg ggtgtgctct gcatacttcg aggtcagcac
     9241 gcgggccagc agccccggtc cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgt
     9301 ctgcacgatg gcatcgcact gagtacaggc atgtttttcc cgtaccgtcc ggataacccg
     9361 gaatgcgcta cgcatcaact ccagctgttc ggcggtatcc tcgcccagat agctcagtga
     9421 accgccgcag ttcgggcagc acggcgccgc aggcaacagt cgcttttcgt cacggggtag
     9481 tgattcaggg aacggcttac gggtgcgggt ctgacgcaac ggacgctgta ctgccgggtc
     9541 atacacccta ccagtcagcg tatcgctctc tttctgaagc cggttcagat cggcttccat
     9601 ttgtgcgata cggcgggaga ctttttcgga acgactgccg aagttcatcc ggcggagttt
     9661 atccagctgc gcctgcagat ggtctatttc gcgctcccgg ttgctcagct tttcctgcag
     9721 ggcgtggatc agcgcttcct gttcggccag gcgctgtttc agcaggaaga tgtcgtcaga
     9781 agagatgtcg ttcataagcc cgtattttac cgggcttatt ctgtgacaac caggataaag
     9841 agatttacag catggtcagg gaggtcagca gccgcttagg ctgtcgccag tcgatacctt
     9901 ccagaagcat cgccagctgc gcctgcgtaa ggaacacttt gccatcacgg gctgacggcc
     9961 aggcgaagcg cccacgctcc agccgtttgg tcaggaggca cagtccgtca ccggtggacc
    10021 acagcagttt aacctgactg ccgctgcggc cccggaaaat gaaaacatgg ccggacatgg
    10081 gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc gttgaagcca tttctcatat
    10141 cggtgatacc ggcaaccagc caaattttgg tcccggaagg taacgggatc atcgcttcag
    10201 ttcctgtatc agcagagtca ggagcttttc gctgacattg ccattgaagc ggagcgtccc
    10261 gtgccggaac gttacctcac agctgatact gagggtttcc gggtcctctg
//
LOCUS       DAZGCS010000144        10305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGCS010000144 DAZGCS010000000
VERSION     DAZGCS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.146"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHK01_004340"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHK01_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO8099175.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHK01_004341"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHK01_004341"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO8099176.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHK01_004342"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHK01_004342"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO8099177.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHK01_004343"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHK01_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO8099178.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHK01_004344"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHK01_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO8099179.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHK01_004345"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHK01_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO8099180.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHK01_004346"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHK01_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO8099181.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLKSAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHK01_004347"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHK01_004347"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO8099182.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHK01_004348"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHK01_004348"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO8099183.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9972..>10305
                     /gene="yfiL"
                     /locus_tag="ACHK01_004349"
     CDS             9972..>10305
                     /gene="yfiL"
                     /locus_tag="ACHK01_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO8099184.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGA"
BASE COUNT         2636 a         2726 c         2425 g         2518 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaaactca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgagga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcat tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttaaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg agagggtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgctta gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agcagcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacacttc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctg
//
LOCUS       DAZGCS010000145        10271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGCS010000145 DAZGCS010000000
VERSION     DAZGCS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.147"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..857)
                     /gene="yaaA"
                     /locus_tag="ACHK01_004350"
     CDS             complement(81..857)
                     /gene="yaaA"
                     /locus_tag="ACHK01_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO8099185.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(927..2357)
                     /locus_tag="ACHK01_004351"
     CDS             complement(927..2357)
                     /locus_tag="ACHK01_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO8099186.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2636..3589
                     /gene="tal"
                     /locus_tag="ACHK01_004352"
     CDS             2636..3589
                     /gene="tal"
                     /locus_tag="ACHK01_004352"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8099187.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3704..4291
                     /gene="mog"
                     /locus_tag="ACHK01_004353"
     CDS             3704..4291
                     /gene="mog"
                     /locus_tag="ACHK01_004353"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO8099188.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4326..4892)
                     /gene="satP"
                     /locus_tag="ACHK01_004354"
     CDS             complement(4326..4892)
                     /gene="satP"
                     /locus_tag="ACHK01_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO8099189.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5041..5754)
                     /gene="msyB"
                     /locus_tag="ACHK01_004355"
     CDS             complement(5041..5754)
                     /gene="msyB"
                     /locus_tag="ACHK01_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8099190.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5780..6184)
                     /locus_tag="ACHK01_004356"
     CDS             complement(5780..6184)
                     /locus_tag="ACHK01_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO8099191.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6561..8477
                     /gene="dnaK"
                     /locus_tag="ACHK01_004357"
     CDS             6561..8477
                     /gene="dnaK"
                     /locus_tag="ACHK01_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO8099192.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8566..9696
                     /gene="dnaJ"
                     /locus_tag="ACHK01_004358"
     CDS             8566..9696
                     /gene="dnaJ"
                     /locus_tag="ACHK01_004358"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO8099193.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9800..9952)
                     /gene="mokC"
                     /locus_tag="ACHK01_004359"
     CDS             complement(9800..9952)
                     /gene="mokC"
                     /locus_tag="ACHK01_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO8099194.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2692 a         2565 c         2652 g         2362 t
ORIGIN      
        1 taattctgca atatattgaa tctgcatgct tttgtaggcc ggataaggcg ttcacgccgc
       61 atccggcatt ggctacaaac ttaacgctgc tcgtagcgtt taaacaccag ttcaccattg
      121 ctggaggaag cttcatcaaa gaagtaacct tcgctattaa aaccagtcag ttgctctggt
      181 ttggtcagcc gattttcaat aatgaaacga ctcatcagac cgcgtgcttt cttagcgtag
      241 aagctgatga tcttaaattt gccgttcttc tcatcgagga acaccggctt gataatctcg
      301 gcattcaatt tcttcggctt caccgattta aaatactcat ctgacgccag attaatcacc
      361 acattatcgc cttgtgctgc gagcgcctcg ttcagcttgt tggtgatgat atctccccag
      421 aattgataca gatctttccc tcgggcattc tcaagacgga tccccatttc cagacgataa
      481 ggctgcatta aatcgagcgg gcgcagtacg ccatacaagc cggaaagcat tcgcaaatgc
      541 tgttgggcaa aatcgaaatc gtcttcgctg aaggtttcgg cctgcaagcc ggtgtagaca
      601 tcacctttaa acgccagaat cgcctggcgg gcattctccg gcgtgaaatc tggctgccag
      661 tcatgaaagc gagcggcgtt gatacccgcc agtttgtcgc tgatgcgcat cagcgtgcta
      721 atctgcggag gcgtcagttt ccgcgcttca tggatcaact gctgggaatt gtctaacagc
      781 tccggcagcg tatagcgcat ggtggtcaac gggctttggt aatcaagcgt tttcgcaggt
      841 gaaataagaa tcagcatatc cagtccttgc aggaaattta tgccgacttt agcaaaaaaa
      901 gagaatgagt tgatcgatag ttgtgattac tcctgcgaaa catcatccca cgcgtccgga
      961 gaaagctggc gaccgatatc cggataacgc aacggatcaa acaccgggcg cacgccgagt
     1021 ttacgctggc gtagataatc actggcaatg gtatgaacca caggcgagag cagtaaaatg
     1081 gcggtcaaat tggtaatagc catgcaggcc attatgatat ctgccagttg ccacatcagc
     1141 ggaagactta gcaaggtgcc gccgatgacc gttgcgaagg tgcagatccg caaacaccag
     1201 atcgctttag ggttgttcag gcgtaaaaag aagagattgt tttcggcata aatgtagttg
     1261 gcaacgatgg agctgaaggc aaacagaata accacgaggg taacaaactc agcaccccag
     1321 gaacccatta gcacccgcat cgccttctgg ataagctgaa taccttccag cggcatgtag
     1381 gttgtgccgt tacccgccag taatatcagc atggcgcttg ccgtacagat gaccagggtg
     1441 tcgataaaaa tgccaatcat ctggacaatc ccttgcgctg ccggatgcgg aggccaggac
     1501 gcagctgccg ctgctgcgtt tggtgtagaa cccattcccg cctcattgga aaacatactg
     1561 cgctgaaaac cgttagtaat cgcctggctt aaggtatatc ccgccgcgcc gcctgccgct
     1621 tcctgccagc caaaagcact ctcaaaaata gaccaaatga cgtggggaag ttgcccgata
     1681 ttcattacgc aaatcaccag gctggtcagt acccagatta tcgccatcaa cgggacaaaa
     1741 ccctgcatga gccgggcgac gccatgaaga ccgcgagtga ttgccagcag agcaaagaca
     1801 gcgagaataa tgcctgtcac cagcggggga aaatcaaaag aaaaactcag ggctcgggca
     1861 acggcgttcg cctgaactcc gctgaaaatt atgccgtagg cgatgagcaa aaagacggcg
     1921 aacagaacgc ccatccaacg catccccagc ccgcgcgcca tataccatgc cggtcctcca
     1981 cgaaactgtc cattgacgtc acgttcttta taaagttgtg caagggaaca ttcggcaaac
     2041 gaggtcgcca tgccgataaa cgcggcaacc cacatccaaa agacggctcc aggtccaccg
     2101 gcggtaatag ccagcgcaac gccggccagg ttgccgctac ccacgcgcgc cgcaagactg
     2161 gtacacaatg actgaaatga ggttaaaccg cctggctgtg gatgaatgct atttttaaga
     2221 cttttgccaa actggcggat gtagcgaaac tgcacaaatc cggtgcgaaa agtgaaccaa
     2281 caacctgcgc cgaagagcag gtaaatcatt accgatcccc aaaggacgct gttaataaag
     2341 gagaaaaaat ctggcatgca tatccctctt attgccggtc gcgatgactt tcctgtgtaa
     2401 acgttaccaa ttgtttaaga agtatatacg ctacgaggta cttgataact tctgcgtagc
     2461 atacatgagg ttttgtataa aaatggcggg cgatatcaac gcagtgtcag aaatccgaaa
     2521 cagtctcgcc tggcgataac cgtcttgtcg gcggttgcgc tgacgttgcg tcgtgatatc
     2581 atcagggcag accggttaca tccccctaac aagctgttta aagagaaata ctatcatgac
     2641 ggacaaattg acctcccttc gtcagtacac caccgtagtg gccgacactg gggacatcgc
     2701 ggcaatgaag ctgtatcaac cgcaggatgc cacaaccaac ccttctctca ttcttaacgc
     2761 agcgcagatt ccggaatacc gtaagttgat tgatgatgct gtcgcttggg cgaaacagca
     2821 gagcaacgat cgcgcgcagc agatcgtgga cgcgaccgac aaactggcag taaatattgg
     2881 tctggaaatc ctgaaactgg ttccgggccg tatctcaact gaagttgatg cgcgtctttc
     2941 ctatgacacc gaagcgtcaa ttgcgaaagc aaaacgcctg atcaaactct acaacgatgc
     3001 tggtattagc aacgatcgta ttctgatcaa actggcttct acctggcagg gtatccgtgc
     3061 tgcagaacag ctggaaaaag aaggcatcaa ctgtaacctg accctgctgt tctccttcgc
     3121 tcaggctcgt gcttgtgcgg aagcaggcgt gttcctgatc tcgccatttg ttggccgtat
     3181 tcttgactgg tacaaagcga atactgataa gaaagagtac gctccggcag aagatccggg
     3241 cgtggtttct gtatctgaaa tctaccagta ctacaaagag cacggttatg aaacagtggt
     3301 tatgggcgca agcttccgta acatcggcga aattctggaa ctggcaggct gcgaccgtct
     3361 gaccatcgca ccggcactgc tgaaagagct ggcggagagc gaaggggcta tcgaacgtaa
     3421 actgtcttac accggcgaag tgaaagcgcg tccggcgcgt atcactgagt ccgagttcct
     3481 gtggcagcac aaccaggatc caatggcagt agataaactg gcggaaggta tccgtaagtt
     3541 tgctattgac caggaaaaac tggaaaaaat gatcggcgat ctgctgtaat cattcttagc
     3601 gtgaccggga agtcggtcac gctacctctt ctgaagcctg tctgtcactc ccttcgcagt
     3661 gtatcattct gtttaacgag actgtttaaa cggaaaaatc ttgatgaata ctttacgtat
     3721 tggcttagtt tccatctctg atcgcgcatc cagcggcgtt tatcaggata aaggcatccc
     3781 tgcgctggaa gaatggctga catcggcgct aaccacgccg tttgaactgg aaacccgctt
     3841 aatccccgat gagcaggcga tcatcgagca aacgttgtgt gagctagtgg atgaaatgag
     3901 ttgccatctg gtgctcacca cgggcggaac tggcccggcg cgtcgtgacg taacgcccga
     3961 tgcgacgctg gcagtagcgg accgcgagat gcctggcttt ggtgaacaga tgcgccagat
     4021 cagcctgcat tttgtaccaa ctgcgatcct ttcgcgtcag gtgggcgtga ttcgcaaaca
     4081 ggcgctgatc cttaacttac ccggtcagcc gaagtctatt aaagagacgc tggaaggtgt
     4141 gaaggacgct gcgggtaacg ttgtggtaca cggtattttt gccagcgtac cgtactgcat
     4201 tcagttgctg gaagggccat acgttgaaac ggcaccggaa gtggttgcag cattcagacc
     4261 gaagagtgca agacgcgacg ttagcgaata aaaaaatccc cccgagcggg gggatctcaa
     4321 aacaattagt gggattcacc aatcggcaga acggtgcgac caaactgctc gttcagtact
     4381 tcacccatcg ccagatagat tgcgctggca ccgcagatca gcccaatcca gccggcaaag
     4441 tggatgattg cggcgttacc ggcaatgtta ccgatcgcca gcagggcaaa cagcacggtc
     4501 aggctaaaga aaacgaattg cagaacgcgt gcgcctttca gcgtgccgaa gaacataaac
     4561 agcgtaaata cgccccacag acccaggtag acaccaagga actgtgcatt tggcgcatcg
     4621 gtcagaccca gtttcggcat cagcagaatc gcaaccagcg tcagccagaa agaaccgtaa
     4681 gaggtgaatg cggttaaacc gaaagtgttg ccttttttgt actccagcag accagcaaaa
     4741 atttgcgcga tgccgccgta gaaaatgccc atggcaagaa taataccgtc cagagcgaaa
     4801 taacccacgt tgtgcaggtt aagcagaatg gtggtcatgc cgaagcccat caggcccagc
     4861 ggtgccggat tagccaactt agtgttgccc ataattcctc aaaaatcatc atcgaatgaa
     4921 tggtgaaata atttccctga ataactgtag tgttttcagg gcgcggcata ataatcagcc
     4981 agtggggcag tgtctacgat cttttgaggg gaaaatgaaa attttccccg gtttccggta
     5041 tcagacctga gtggcgctaa ccatccggcg caggcaggcg atttgcagta cggctggaat
     5101 cgtcacgcga taggcgctgc cgctgaccgc tttaacccca tttagtgccg cacctacagg
     5161 gcctcccagc cccgcgccgc gcagcaaacc atgcccaagt acgctcattg ctgcgtgggt
     5221 gcgtaaaatg cgggtcagtt ggctggaaag caaatgcgac acgccttttg ccaataattt
     5281 gtctttcatc agcagcggca gcagctcttc cagctcattc accctggcat cgatcgcgtg
     5341 cagaaactcc tgcttatgtt cctcgtccat attcttccag gtgttacgca gaaattgttc
     5401 cagtaactgt tgctcaatct caaacgtaga catctctttg tcggctttca gcttcaatcg
     5461 ttttgaaaca tcgagcaaaa tggcccgata caatttaccg tgtccgcgca gtttgttggc
     5521 gatactatcg ccaccaaaat gctgtaattc tccagcaatc agctgccagt tgcggcgatg
     5581 ttgctcggga tgcccttcca tcgatttaaa cagttcgttg cgcatcagta cgctggagag
     5641 gcgagttttg cctttttcat tatgggtgag caatcgggcg aaatttgcca actgttcctc
     5701 actgcaatgc tggagaaaat ccagatctga atcattcagg taattaacat tcattttttg
     5761 tggcttctat attctggcgt tagtcgtcgc cgataatttt cagcgtggcc atatccgatg
     5821 agttcaccgt atgaccggaa aaggtgattt ttgagacgca gcgtttattg tcgttatcgc
     5881 tgttaatgtt gatccagtca gtggtttgcc cttcttttat ttctgaagga atattcaggc
     5941 tctgactggc gctacgggcg gctttgaaat aaaccgatgc accgcttaac tgtaaatcgc
     6001 catggtcggc agagagttgt atgcgtttaa caattcggca aacaggaagt ttcaacgcca
     6061 gatcgttggt ttcgttacgc ggcattgcaa tgacgccgag gattttatgg tcgtttgcct
     6121 gcgccgtgca gcacagcatc agactaatcg ccaggctggc ggaaatcgta aaaacggatt
     6181 tcataaggat tctcttagtg gggagaggta gggggatgga tacccactag tttactgctg
     6241 ataaagagaa gattcaggtg cgtaatgttt tctttttatt acaatttttt gatgaatgtc
     6301 ttggctgcga ttcattcttt atatgaataa aattgctgtc agtttcacgt cttgtcctgc
     6361 tatatcgcga aatttctgcg caaaagcaca aaaaattttt gcatctcccc cttgatgacg
     6421 tgggttacga ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt
     6481 gggcagttga aaccagacgt ttcgccccta ttacagactc acaaccacat gatgaccgaa
     6541 tatatagtgg agacgtttag atgggtaaaa taattggtat cgacctgggt actaccaact
     6601 cttgtgtagc gattatggat ggcaccactc ctcgcgtgct ggagaacgcc gaaggcgatc
     6661 gcaccacgcc ttctatcatt gcctataccc aggatggtga aactctggtt ggtcagccgg
     6721 ctaaacgtca ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg
     6781 gccgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta
     6841 ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc
     6901 agatctctgc tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ttgggtgaac
     6961 cggtaactga agctgttatc accgtaccgg catactttaa cgatgctcag cgtcaggcaa
     7021 ccaaagacgc aggccgtatc gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg
     7081 cagctgcgct ggcttacggt ctggacaaag gtactggcaa ccgtactatc gcggtttatg
     7141 acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa
     7201 aaaccttcga agttctggct accaacggtg atacccacct gggtggtgaa gacttcgaca
     7261 gccgtctgat caactacctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca
     7321 acgatccgct ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt
     7381 cttccgctca gcagaccgac gttaacctgc cgtacatcac tgcagatgcg accggtccga
     7441 aacacatgaa catcaaagtg actcgtgcga aactggaaag cctggttgaa gatctggtaa
     7501 accgttccat tgagccgctg aaagttgcac tgcaggacgc tggcctgtcc gtatctgata
     7561 tcgacgacgt tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg
     7621 ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg
     7681 gtgctgctgt tcagggtggt gttctgactg gtgacgtgaa agacgtactg ctgctggacg
     7741 ttaccccgct gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga
     7801 aaaacaccac tatcccgacc aagcacagcc aggtgttctc taccgctgaa gacaaccagt
     7861 ctgcggtaac catccatgtg ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc
     7921 tgggtcagtt caacctggat ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag
     7981 ttaccttcga tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg
     8041 gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga
     8101 aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac
     8161 agactcgcaa ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag
     8221 gcgacaaact gccggctgac gacaaaactg ctatcgagtc tgcactgact gcactggaaa
     8281 ctgctctgaa aggtgaagac aaagccgcta tcgaagcgaa aatgcaggag ctggcacagg
     8341 tttcccagaa actgatggaa atcgcccagc agcaacatgc ccagcagcag actgccggtg
     8401 ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag
     8461 tcaaagacaa aaaataatcg ccctataaac gggtaattat actgacacgg gcgaagggga
     8521 atttcctctc cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc taagcaagat
     8581 tattacgaga ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcaa aaaggcctac
     8641 aaacgcctgg ccatgaaata ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg
     8701 aaatttaaag agatcaagga agcttatgaa gttctgaccg actcgcaaaa acgtgcggca
     8761 tacgatcagt atggtcatgc tgcgtttgag caaggtggta tgggcggcgg cggttttggc
     8821 ggcggcgcag acttcagcga tatttttggt gacgttttcg gcgatatttt tggcggcgga
     8881 cgtggtcgtc aacgtgcggc gcgcggtgct gatttacgct ataacatgga gctcaccctc
     8941 gaagaagctg tacgtggcgt gaccaaagag atccgcattc cgactctgga agagtgtgac
     9001 gtttgccacg gtagcggtgc aaaaccaggt acacagccgc agacctgtcc gacctgtcat
     9061 ggttctggtc aggtgcagat gcgtcaggga ttcttcgctg tacagcagac ctgtccacac
     9121 tgtcagggcc gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt
     9181 gttgagcgca gcaaaacgct gtccgttaaa atcccggcag gggtggacac tggagaccgc
     9241 atccgtcttg cgggcgaagg tgaagcgggc gagcatggcg caccggcagg cgatctgtac
     9301 gttcaggttc aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa cctctattgc
     9361 gaagtcccga tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt accgaccctt
     9421 gatggtcgcg tcaaactgaa agtgcctggc gaaacccaga ccggtaagct tttccgtatg
     9481 cgcggtaaag gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt
     9541 gtcgtcgaaa caccggtagg cctgaacgag aagcagaaac agctgctgca agagctgcaa
     9601 gaaagcttcg gtggcccaac cggtgagcac aacagcccgc gctcaaagag cttctttgat
     9661 ggtgtgaaga agttttttga cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg
     9721 caggcctggg taaaaatagg gtgcgttgaa gatatgcgag cacctgtaaa gtggcgggga
     9781 tcactccccg ccgttgctct tactcggatt cgtaagccgt gaaaacagca acctccgtct
     9841 ggccagttcg gatgtgaacc tcacagaggt cttttctcgt taccagcgcc gccactacgg
     9901 cggtgataca gatgacgatc agggcgacaa tcatcgcctt atgctgcttc attgctctct
     9961 tctccttgac cttacggtca gtaagaggca ctctacatgt gttcagcata tagggggcct
    10021 cgggttgatg gtaaaatatc actcggggct tttctctatc tgccgttcag ctaatgcctg
    10081 agacagacag cctcaagcac ccgccgctat tatatcgctc tctttaaccc attctgtttt
    10141 atcgattcta atcctgaaga cgcctcgcat ttttatggcg taatttttta atgatttaat
    10201 tatttaactt taatttatct cttcatcgca attattgacg acaagctgga ttatttttga
    10261 aatattggcc t
//
LOCUS       DAZGCS010000146        10220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGCS010000146 DAZGCS010000000
VERSION     DAZGCS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.148"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1186
                     /gene="ybaT"
                     /locus_tag="ACHK01_004360"
     CDS             <1..1186
                     /gene="ybaT"
                     /locus_tag="ACHK01_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8099195.1"
                     /translation="QAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDF
                     FRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGI
                     IAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFF
                     SCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSA
                     LELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERE
                     LPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRL
                     RHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNK
                     TV"
     gene            1217..1314
                     /locus_tag="ACHK01_004361"
                     /pseudo
     CDS             1217..1314
                     /locus_tag="ACHK01_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1311..1718
                     /gene="cueR"
                     /locus_tag="ACHK01_004362"
     CDS             1311..1718
                     /gene="cueR"
                     /locus_tag="ACHK01_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO8099196.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1719..2177)
                     /gene="ybbJ"
                     /locus_tag="ACHK01_004363"
     CDS             complement(1719..2177)
                     /gene="ybbJ"
                     /locus_tag="ACHK01_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO8099197.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2174..3091)
                     /gene="qmcA"
                     /locus_tag="ACHK01_004364"
     CDS             complement(2174..3091)
                     /gene="qmcA"
                     /locus_tag="ACHK01_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO8099198.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3237..3914
                     /gene="fetA"
                     /locus_tag="ACHK01_004365"
     CDS             3237..3914
                     /gene="fetA"
                     /locus_tag="ACHK01_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO8099199.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3901..4680
                     /gene="fetB"
                     /locus_tag="ACHK01_004366"
     CDS             3901..4680
                     /gene="fetB"
                     /locus_tag="ACHK01_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO8099200.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4743..5597)
                     /gene="cnoX"
                     /locus_tag="ACHK01_004367"
     CDS             complement(4743..5597)
                     /gene="cnoX"
                     /locus_tag="ACHK01_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO8099201.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5658..6467)
                     /gene="ybbO"
                     /locus_tag="ACHK01_004368"
     CDS             complement(5658..6467)
                     /gene="ybbO"
                     /locus_tag="ACHK01_004368"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8099202.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6457..7080)
                     /gene="tesA"
                     /locus_tag="ACHK01_004369"
     CDS             complement(6457..7080)
                     /gene="tesA"
                     /locus_tag="ACHK01_004369"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO8099203.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7051..7737
                     /gene="ybbA"
                     /locus_tag="ACHK01_004370"
     CDS             7051..7737
                     /gene="ybbA"
                     /locus_tag="ACHK01_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO8099204.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLGHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7734..10148
                     /gene="ybbP"
                     /locus_tag="ACHK01_004371"
     CDS             7734..10148
                     /gene="ybbP"
                     /locus_tag="ACHK01_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO8099205.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLLLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHLELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2231 a         2634 c         2862 g         2493 t
ORIGIN      
        1 gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt
       61 ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg
      121 cattatcgac ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt
      181 actgtacctg ttgacgctgg cggtgagcat cgccatggtc gcccgtgctt ttggcgctta
      241 tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt
      301 ggggatcatt gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct
      361 ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt
      421 ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt
      481 ctcctgtatc gggataacct tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc
      541 ggataaagtg aaagatccgc aggtcattat gccgcgggcg tttctggtgg cgattggcgt
      601 taccacgttg ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga
      661 gttagaaaaa tatgccgata ccgccgttgc gcaggctgct tctccgctgc tcggacatgt
      721 gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa
      781 cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct
      841 aatgaataaa tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat
      901 tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca gcgccacctt
      961 tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc
     1021 ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat
     1081 ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact
     1141 cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg
     1201 cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt
     1261 gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca
     1321 gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat gaagagaagg
     1381 ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc
     1441 tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg
     1501 gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca
     1561 cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc
     1621 agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg
     1681 aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata
     1741 tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg
     1801 ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc
     1861 ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta
     1921 tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg
     1981 agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag
     2041 accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc
     2101 agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg
     2161 accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcggcaa
     2221 tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt
     2281 tactggagga accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca
     2341 ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg
     2401 cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg
     2461 ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc
     2521 cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg
     2581 aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc
     2641 cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct
     2701 gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca
     2761 ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc gcgtcaatca
     2821 cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct
     2881 gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa
     2941 acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta
     3001 ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga
     3061 caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata
     3121 atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc
     3181 cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagtgatgc
     3241 aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga
     3301 ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt
     3361 ccggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg
     3421 gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccagc
     3481 aagtctctta ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataatctga
     3541 tctttccctg gcagatccgt aaccagcagc ctgacccggc catttttctc gattttctcg
     3601 aacgcttcgc tttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg
     3661 aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg
     3721 atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc
     3781 gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta
     3841 atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct
     3901 atgaactcgc ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg
     3961 gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc
     4021 gggcgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg
     4081 gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac
     4141 gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg
     4201 gtcggggcgg gaattaccct ggcggtgctg attctctctg ggtcgattga atttatcccg
     4261 atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg
     4321 tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg
     4381 agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg
     4441 gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg
     4501 atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg
     4561 gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc
     4621 tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga
     4681 tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa
     4741 tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta
     4801 cccagcgcag cgaggatctc ctggaacgtt ttacgtgtct gaccgtctgc ggcggtgaga
     4861 tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctggtgc
     4921 agttgcagag ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt
     4981 tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc
     5041 agcccctggt agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca
     5101 tcttcagaac ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc
     5161 tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc
     5221 tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc
     5281 agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg
     5341 ttctggaaca gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc
     5401 tccgcgtcac agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt
     5461 tccaggatcg gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc
     5521 accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca
     5581 atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct
     5641 taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc cggcagcagg
     5701 cgcttgagca ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc
     5761 tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg
     5821 gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg
     5881 aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgcagc
     5941 tccatgcgca gtgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca
     6001 cgacccggcg tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg
     6061 tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg
     6121 gaaaactgct gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat
     6181 ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgattac ctcgtctgct
     6241 gcgcgatcaa cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg
     6301 ttcatacgct caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt
     6361 tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat
     6421 ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg
     6481 ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat
     6541 accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag
     6601 cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg
     6661 gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt
     6721 gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa
     6781 accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt tcagcagagc
     6841 cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga
     6901 cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg
     6961 atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa
     7021 ggttaacagg accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat
     7081 catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag
     7141 ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca
     7201 accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg
     7261 ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc
     7321 ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag
     7381 cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg
     7441 ctcgaacagt tagggctggg taaacgtctg ggtcatcttc cggcacagct ttccggcggt
     7501 gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc
     7561 gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt
     7621 tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg
     7681 gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg
     7741 cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa
     7801 gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct
     7861 taagccaaca aagccgggag tttatggcgg gcgatcgggc gttgcgcagt tcgcgcgaag
     7921 tgccgcaagc atggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga
     7981 ctttcgccac catgacattt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg
     8041 atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg
     8101 cgggcagcgt attgctggcc ccacgcctga tggcgctgct taacctgaaa acgggcgaca
     8161 ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt
     8221 ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata
     8281 aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ctataaattc ggcggcagtg
     8341 agaaccagct cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct
     8401 ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt
     8461 tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcagtg gcggtagcga
     8521 tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag
     8581 ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg ttgctttcgg
     8641 ccgttaccgg tggggcgata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc
     8701 ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgctcggca
     8761 ccatgacagt catctcgctg ctggtggggc tacgaccgta ccgcttgttg ctggcaacgc
     8821 agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc
     8881 tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggc ggcagtatgc
     8941 tgctctgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gttctaggct
     9001 ggatgctgct gaatgtactt cgccgcatga cgctgaaatc gctgcctctg cgcctggcgg
     9061 ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct
     9121 cctttatgct gttggcactg ctgctggtgt tgcgtggtga tctgctcgac cgctggcagc
     9181 agcagctacc tccagaaagc ccgaactact ttttaattaa catcgccaca gaacaggtag
     9241 caccgctaaa agcgttcctc gcggaacatc agatagtccc ggaatcgttt tatccggtgg
     9301 tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa gatgaggcgc
     9361 ttaaccgcga actcaatctt acctggcaaa atacgcggcc cgatcataat ccgattgtcg
     9421 ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg gcgaaacgct
     9481 taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag ttccgcgcta
     9541 aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc tattttattt
     9601 tccctgaagg ggcattagac gggcaaccgc agagctggct taccagtttc cgctgggaga
     9661 atggcaacgg catgttgaca caactcaacc gccagttccc gaccattagt ctgttagata
     9721 ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg gcgctggaag
     9781 tgatggtggt actggtcacc gcctgcggta tgttgctgtt gctggctcag gtgcaggtgg
     9841 gaatgcgtca gcgtcatctg gagctggtgg tgtggcgcac actcggtgcg gggaaaaagc
     9901 tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg gtttgtttct ggcctggtag
     9961 ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt gattttccgt
    10021 gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcgctg ctgctgtcgc
    10081 ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc aggcagtttg
    10141 cggggtgaag agagtaaata aggggcgttc tggttgaatg gaacgcctag tttaaattaa
    10201 ttctacaata tgtttcattg
//
LOCUS       DAZGCS010000147        10097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGCS010000147 DAZGCS010000000
VERSION     DAZGCS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.149"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..97)
                     /gene="ykiE"
                     /locus_tag="ACHK01_004372"
     CDS             complement(29..97)
                     /gene="ykiE"
                     /locus_tag="ACHK01_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO8099206.1"
                     /translation="MGWSLCFWHVSVKMTHDVVCYY"
     gene            234..755
                     /gene="fecI"
                     /locus_tag="ACHK01_004373"
     CDS             234..755
                     /gene="fecI"
                     /locus_tag="ACHK01_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO8099207.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            752..1705
                     /gene="fecR"
                     /locus_tag="ACHK01_004374"
     CDS             752..1705
                     /gene="fecR"
                     /locus_tag="ACHK01_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO8099208.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGASGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1792..4116
                     /gene="fecA"
                     /locus_tag="ACHK01_004375"
     CDS             1792..4116
                     /gene="fecA"
                     /locus_tag="ACHK01_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO8099209.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPALAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRSYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYGSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4161..5063
                     /gene="fecB"
                     /locus_tag="ACHK01_004376"
     CDS             4161..5063
                     /gene="fecB"
                     /locus_tag="ACHK01_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO8099210.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5060..6058
                     /gene="fecC"
                     /locus_tag="ACHK01_004377"
     CDS             5060..6058
                     /gene="fecC"
                     /locus_tag="ACHK01_004377"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO8099211.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6055..7011
                     /gene="fecD"
                     /locus_tag="ACHK01_004378"
     CDS             6055..7011
                     /gene="fecD"
                     /locus_tag="ACHK01_004378"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO8099212.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIYPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7012..7779
                     /gene="fecE"
                     /locus_tag="ACHK01_004379"
     CDS             7012..7779
                     /gene="fecE"
                     /locus_tag="ACHK01_004379"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO8099213.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGHLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8315..8719)
                     /locus_tag="ACHK01_004380"
     CDS             complement(8315..8719)
                     /locus_tag="ACHK01_004380"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO8099214.1"
                     /translation="MMRGILPPVSVCRMAIPWWITRIHGVTTSAPLITGAGGGVGVSI
                     GAGGNGAGISVFASVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGN
                     RIIADVGHDLLISSQQDTSKYDSKQTSVAAGD"
     gene            complement(8601..9629)
                     /locus_tag="ACHK01_004381"
                     /pseudo
     CDS             complement(8601..9629)
                     /locus_tag="ACHK01_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
BASE COUNT         2249 a         2884 c         2740 g         2224 t
ORIGIN      
        1 atccccatcc tttagggcag tgagaatgtc aataatagca gaccacatca tgagtcattt
       61 tgaccgacac atgccagaag cagaggctcc atcccatttt atacctaccc tataacactt
      121 agaaaaacaa catgttaaaa atgtctattg gaaacaattt tatttccaat tgtaatgata
      181 accattctca tattaatatg gctacgtgat aattaacttt tgatgcactc cgcatgtctg
      241 accgcgccac taccacagct tccttaacgt tcgagtcgct ttatggcaca catcacggct
      301 ggttgaaaag ctggctgacg cgcaaactcc agtctgcttt tgatgcagat gacattgccc
      361 aggacacttt tttgcgggta atggtcagcg aaacgctctc gacgatccgc gatcctcgct
      421 ccttcctgtg cactatcgcc aaacgcgtga tggtggacct gtttcgccga aacgcgctgg
      481 aaaaagcgta tctggagatg ctggcgctta tgccggaggg gggagcgcct tcacctgagg
      541 aacgcgaaag ccaactcgag accctacaac tcctcgacag catgctggac gggctaaacg
      601 gcaaaacacg tgaagcgttt ctgctttcgc aactggatgg tctgacatac agcgagattg
      661 cgcacaaact cggtgtttcc atcagctccg tgaaaaaata cgtggcgaaa gccgtcgagc
      721 actgcctgct gttccgtctg gagtatgggt tatgaatcct ttgttaaccg attcccgccg
      781 tcaggcgctg cgttcagctt cccactggta tgccgtgcta agcggcgagc gcgtcagccc
      841 acaacaggaa gcgcgctggc aacagtggta tgaacaggat caggataacc agtgggcctg
      901 gcagcaggtt gaaaacctgc gcaaccagct tggcggtgtg cctggcgacg ttgccagccg
      961 ggcgttgcac gatacccgcc tcacccgccg tcacgtgatg aaaggattac tgctgttgct
     1021 cggcgctagc ggaggctggc agctctggca gtcggaaacc ggcgaaggtc tgcgggcaga
     1081 ttaccgcacc gccaaaggca cagtcagccg tcagcaactg gaagatggct ccctgctcac
     1141 gctgaatacc caaagcgcgg cggatgtgcg ttttgatgcg catcagcgca ccgtccggct
     1201 ctggtacggt gaaatcgcca ttactaccgc gaaagatgcc ctgcaacgcc ccttccgtgt
     1261 cctgacccgt cagggccagc tcactgcttt agggacagaa tttaccgtcc gccagcagga
     1321 taatttcacg cagcttgacg tgcagcagca cgccgtggaa gtgcttctcg ccagtgcccc
     1381 cgcgcaaaaa cgcatcgtga acgctggtga aagcctgcag ttcagcgcct ctgagtttgg
     1441 cgcagtgaaa ccgctggatg acgagagtac aagctggacg aaggacatcc tgagcttcag
     1501 cgataaaccg ctgggtgagg tgatagccac gctaacccgt taccgcaacg gcgtgctgcg
     1561 ctgcgatccc gccgttgccg ggctgcgcct gagcgggacg ttcccgctga aaaataccga
     1621 tgcgatcctg aacgttatcg cgcaaacgct tcccgttaaa attcagtcta ttacgcggta
     1681 ctggataaac atttcaccac tgtaaggaaa ataattctta tttcgattgt cctttttacc
     1741 cttctcgttc gactcatagc tgaacacaac aaaaatgatg atggggaagg tatgacgccg
     1801 ttacgcgttt ttcgtaaaac aacacctttg gttaacgcca ttcgcctgag cctgctgccg
     1861 ctggccggtc tctcgttttc cgcttttgct gcacaggtta atatcgcacc gggatcgctc
     1921 gataaagcgc tcaatcagta tgccgcacac agcggaatta ccctctcggt tgacgccagc
     1981 ctgacgcgcg gcaagcagag caacggcctg cacggcgatt acgacgtcga gagcggcctg
     2041 caacaactgc tggacggcag cggactgcag gtaaaaccgc tgggaaataa cagctggacg
     2101 ctggagcccg cgctcgcacc aaaagaagat gccctgaccg tggtcggcga ctggctgggt
     2161 gatgcgcgtg aaaacgacgt atttgaacat gctggcgcgc gtgacgtgat ccgccgtgag
     2221 gatttcgcca aaaccggcgc aaccaccatg cgtgaggtac ttaaccgcat ccctggcgtc
     2281 agcgcgccgg aaaacaacgg caccggcagc cacgacctgg cgatgaactt tggcatccgg
     2341 ggcctgaacc cgcgcctcgc cagccgctcg accgtcctga tggacggcat ccccgtcccc
     2401 ttcgcccctt acggtcagcc gcagctttca ctggctcccg tttcgctcgg caacatggat
     2461 gccattgacg tggtacgcgg tggtggtgcg gtgcgttacg gaccgcagag cgtgggcggc
     2521 gtggtgaact ttgttacccg tgccattccg caggactttg gtatcgaggc gggcgtggaa
     2581 ggtcagctca gcccaacctc ttcacaaaac aacccgaaag agacgcacaa cctgatggtg
     2641 ggcggcacag cggacaacgg ttttggcacc gcgctgctct actccggcac gcgcggcagt
     2701 gactggcgcg agcacagcgc cacccgcatc gacgacctga tgctgaaaag caaatatgcg
     2761 ccggatgagg tgcacacctt caacagcctg ctgcaatatt acgacggtga agccgacatg
     2821 cccggtggcc tgtctcgcgc ggattacgac gccgatcgct ggcaatccac ccgctcgtat
     2881 gaccgcttct ggggtcgtcg caagctggcg agcctgggct accagttcca gccagacagc
     2941 cagcataaat tcaacattca ggggttctac acccaaaccc tgcgcagcgg ctacctggag
     3001 caaggcaaac gcatcaccct ctcgccgcgt aactactggg tgcgcggtat tgagccacgc
     3061 tacagccaga tctttatgat cggcccttcc gcgcacgaag tgggcgtggg ctatcgctat
     3121 gtgaatgaat caacgcatga aatgcgttac tacaccgcca ccagcagcgg gcagttgccg
     3181 tccggctcaa gcccttacga ccgcgatacg cgttccggca ccgaggcgca cgcctggtat
     3241 ctggatgaca aaatcgacat cggcaactgg accatcacgc cgggtatgcg tttcgaacat
     3301 atcgagtcat accagaacaa cgccatcaca ggcacgcacg aagaagtgag ctataacgca
     3361 ccgcttccgg cgttgaacgt gctctatcac ctgactgaca gctggaatct ttatgcaaac
     3421 actgaaggct cgttcggcac cgtacagtac agccagattg gcaaggctgt gcaaagcggc
     3481 aatgtggaac cggaaaaagc gcgaacctgg gaactcggta cccgctacga cgacggcgcg
     3541 ctgacggcgg aaatggggct gttcctgatt aactttaaca atcagtacgg ctccaaccag
     3601 accaacgaca ccgtcactgc acgtggcaaa acgcgccata ccgggctgga aacgcaggca
     3661 cgttacgatc tgggtacgct aacgccaacg cttgataacg tttccatcta cgccagctat
     3721 gcgtatgtga acgcggaaat ccgcgagaaa ggcgacacct acggcaatct ggtaccattc
     3781 tccccgaaac ataaaggcac gctgggcgtg gactacaagc caggaaactg gacgttcaat
     3841 ctgaacagcg atttccagtc cagccagttt gcggataacg ccaatacggt gaaagagagc
     3901 gccgacggca gtaccggccg cattcccggc ttcatgctct ggggcgcacg cgtggcgtat
     3961 gactttggcc cgcagatggc agatctgaac ctggcattcg gtgtgaaaaa catcttcgac
     4021 caggactact tcatccgctc ttatgacgac aacaacaaag gcatctatgc aggccagccg
     4081 cgcacgctgt atatgcaggg gtcgttgaag ttctgatgtg ttaacgcccg gcttgccggg
     4141 cttttagctg gaatgtgatt atgttggcat ttatccgttt tctttttgca ggcctgctgc
     4201 tggtgatcag ccacgccttt gccgccacgg ttcaggacga acacggcacg tttacactcg
     4261 aaaaaacgcc acaacggatt gtggtgctgg aactctcgtt cgccgatgcg ctggccgccg
     4321 tggacgtcag cccgatcggt attgccgacg ataacgatgc aaaacgcatc ctgcccgaag
     4381 tgcgtgcgca cctgaaaccg tggcagtccg tcggaacgcg cgcgcagccg agcctggaag
     4441 ccattgccgc tctgaaacca gacctgatca ttgccgacag cagtcgccat gcgggggttt
     4501 acatcgcctt gcagcaaatc gcgccggtac tgctgcttaa gtcccgcaac gaaacctacg
     4561 ctgaaaattt gcaatctgcg gctatcatcg gcgaaatggt gggtaaaaag cgagagatgc
     4621 aggcacgtct ggaacaacat aaagagagga tggcgcagtg ggccagccag cttcccaaag
     4681 ggacacgcgt ggcctttggc acatcacgcg aacagcaatt caacctgcat actcaggaga
     4741 cctggaccgg cagcgtgctg gcctctctgg ggctgaacgt tcccgctgcg atggcgggtg
     4801 cgtccatgcc gtccatcggc ctggagcaac tgctggcggt caatcctgcc tggctgctgg
     4861 ttgcccacta tcgcgaagag agcattgtta aacgctggca acaagatccg ctctggcaga
     4921 tgttaaccgc cgcgcagaag cagcaggttg cttcggtcga cagtaacacc tgggcgcgga
     4981 tgcgcggtat ttttgctgca gagcgtattg ccgctgacac ggtaaaaatc ttccaccatc
     5041 agccgcttac cgttgtgaaa tgaccgcgat aaaacacccg gtgctgctgt gggggcttcc
     5101 cgttgcagca cttattatta ttttctggct gagtctgttt tgctactcgg ccattcctgt
     5161 ttccggagca gatgcaaccc gcgccctgct gcctggacac acgccaacgc taccagaagc
     5221 gctggtgcaa aaccttcgtt tgccacgaag cctcgtcgcc gttctgatcg gcgcaagcct
     5281 ggcgctcgcg ggcacgctgc tgcaaaccct gacccacaac ccaatggcct ctccttcact
     5341 gctcggcatt aacagcggcg cggcgctggc tatggcgctt accagcgcgc tgagtccgac
     5401 gccgattgca ggctattctc tgtcgttcat cgcggcatgc gggggcggcg tgagctggct
     5461 gctggtcatg accgcaggag gcggatttcg tcatacccat gacagaaaca aactgatcct
     5521 cgcgggtatc gcgctgtcgg ccttttgtat gggccttacc cgcatcaccc tgctgctggc
     5581 cgaagatcat gcttacggca tcttttactg gctggcaggc ggagtgtccc acgcccgctg
     5641 gcaggatgtc tggcagctct tgccggtggt ggtcactgca gtccctgtcg tgttgctgct
     5701 ggcgaatcaa ctgaacctgc tcaacctcag cgacagcacc gcccatacgc tgggagtgaa
     5761 cctgacgagg ctacgtttgg tcatcaatat gttagtgctg cttctggttg gtgcgtgcgt
     5821 cagcgtggca ggtccggtgg cgtttatcgg tctgctggtg ccacatctgg cgcgcttctg
     5881 ggcaggcttc gatcagcgca acgtactgcc agtgagcatg ctgctggggg ccacgctgat
     5941 gctgctggca gatgtactcg cacgcgcgct ggccttcccc ggagatctgc ccgcaggcgc
     6001 agtgctggcg ctgattggca gcccttgctt tgtctggctt gtgaggaggc gaggatgaaa
     6061 attgcgctgg ttattttcat cacccttgcc ctggcgggct gtgcgctgtt atcactccat
     6121 atgggagtga tccccgtgcc gtggcgcgcg ctgctgaccg actggcaggc cggacgcgag
     6181 cattattatg tattgatgga gtaccgactg ccgcgcttgc tgttggcact gtttgtcggt
     6241 gcagccctcg ccgtggcggg tgtgctgata caggggattg tgcgcaaccc tctggcatca
     6301 ccggatattc tcggcgttaa ccatgccgcc agcctggcct ctgtgggggc tctacttctt
     6361 atgccgtcac tgcccgtgat ggtgctgccg ctgctggcct ttgcgggcgg catggcgggg
     6421 ttgatattgc tgaagatgct ggcaaagacc caccagccga tgaagctggc gctcaccggc
     6481 gtggcgcttt ctgcatgctg ggccagcctg acggattatc tgatgctctc gcgcccgcag
     6541 gatgtgaaca acgccctgct gtggctgacc ggcagcttat ggggccgtga ctggagcttt
     6601 gtgaagattg ccatcccgct gatgatttta tttctgccgc tgagcctgag tttttgccgc
     6661 gatctcgacc tccttgcact cggcgatgcg cgcgccacca cgctcggtgt gtcggtgccc
     6721 catacccgat tctgggcttt gttactagct gtcgccatga catctaccgg cgtggccgcc
     6781 tgcggcccga ttagctttat tggtctcgtg gtgccgcata tgatgcgtag catcaccggt
     6841 ggacgtcacc gcagactgct gcctgtttcg gccctgacag gtgcgttgct gttggtggtt
     6901 gccgatctgc tggcgagaat tatttatccc ccactggagc tcccggttgg cgtgctgacc
     6961 gccattatcg gtgcgccgtg gtttgtctgg ttgcttgtga gaatgcgata aatgacttta
     7021 cgaactgaaa atctgacggt cagttacggg acagacaagg tacttaacga cgtttcactc
     7081 tcactgccaa cggggaagat caccgccctg atcggtccta acggttgcgg gaaatcgacg
     7141 ctgttaaact gtttttcgcg gcttttaatg ccgcagtctg gcaccgtatt tctcggcgat
     7201 aatcccataa atatgctctc atcgcgccag ttggcccgca ggctttcgct gctgcctcag
     7261 caccatttaa cgccagaggg gatcacagtc caggagctgg tttcgtatgg tcgtaatccc
     7321 tggctgtcac tctgggggca tctctccgct gaagacaatg cacgagttaa tgtcgccatg
     7381 aaccagaccc ggattaatca tcttgccgtt cgtcggttaa ccgagctttc cggcggtcag
     7441 cgccagcgcg catttctggc gatggtcctg gcccagaata cgcccgttgt attacttgat
     7501 gaaccaacca cctatcttga tatcaatcac caggtggacc tgatgcggtt gatgggcgaa
     7561 ctccggactc aggggaaaac ggtggtcgct gtgctgcacg accttaatca ggctagccgg
     7621 tactgcgatc aactggtggt aatggcaaac gggcatgtta tggcgcaagg cacaccagaa
     7681 gaggtgatga ccccaggatt gctgagaaca gtattcagcg tggaagcgga aatacacccc
     7741 gagccggtat ctggcaggcc gatgtgccta atgaggtaga ttgcacaggc cgtaagaacc
     7801 aaaccacgac tgaatgaaac tggactggcg ccagcaagcc agttcagact ggggctgaac
     7861 ttttccggac tctgaaagat taccaatact catcgtccat ccgcttgctt taggctgaca
     7921 ggttcataat caacgcaaac cagagctgta caggcttggg cgcggctttc aaaccagtcg
     7981 tgaacggcaa tcaattttga actctgctta acggacattt ctgtataacc cttacggcaa
     8041 cgaaaaacgc gaagttaaaa ttttagaaac ccaaaaacgt gacatgacta agtttagatt
     8101 tcagggggga gatcaaaaaa tttcgctctg tgccagagcg gacattcatg gagctggttc
     8161 attaccaatg aggttgggct tttgaggata aatcaatgat cagacgccaa cgtaaatcaa
     8221 aagcaaccct ggaacggaat tgctaatcca gtttctgacc atcgattttt ctaaaaagtg
     8281 tgcgtttgct actacttagg taggtgcagc tttcttaatc accggcagcc acgctggtct
     8341 gtttactgtc gtacttactg gtgtcctgct ggctgcttat cagcaggtcg tggcccacat
     8401 cggcgataat cctgttgccg ttgacctgag caccgttcag taccgtatcc cgaccactgt
     8461 tgatggtgac ggttttaccg ctgtctgttg tggtttcagt ccactcagta ccgttacctt
     8521 tctcgctgcc ttttgccgca ttaacgctgg caaagacgct gataccggca ccgttaccac
     8581 cggcaccgat actgacaccc acgccaccgc cagccccggt tatcaatggt gctgaggtag
     8641 tcactccatg aatacgtgta atccaccagg gtatagccat acggcagact gataccggcg
     8701 gcaaaattcc tcgcatcatg tgacaccgaa aagtcactgc tccgtccccc gctgacaaac
     8761 cagttgtcag ccagccccag aggattatta aaggaaagga caccatttaa ctgccccgta
     8821 ccggtattct tctgcccgct gttgtcgatg cccacgctcc ctgtgactgg ccattccggc
     8881 aatgccgtca gtgtcaccac cgaccatccc tcacggtcac cgggcgatat ttcaatctgt
     8941 accggttccg tacgcagacg attaatctgc tccatcccct gctcaatatc ccgcaggttc
     9001 agaaccttcc cctccattcc cgggaaaacc atcttcaggg tgcgggcagg aaggtcagcg
     9061 ccttccgccc ggatttgctg cagcctgcct tccatgaccg ttatgtgcag tacgccccct
     9121 gaaaggtcct gctctgtcag aaaggcccgg ctggtgatat atccccggcg tatatagccg
     9181 tccgtcacgg catccgtgac cgcagtcagg ccggtgatat tcagacactg attcacccac
     9241 ggtgccacca gtctgtcggt ttctgcagac gtcagtcggg tggccccact gacaacaatg
     9301 cgtgacaccg taaagcaggg gccttcagta ccggcagacg tttccggaga cggcgtgata
     9361 gtcagcggcg cactgcgctc cagcgcatca cgctgatgct ggttttcatc cagcaactgc
     9421 tgctgttgct gctgaattgc actgcggtca cccggagaca gcatggctgc cggagacgaa
     9481 aatgcgggga taaaaaaacc agaaaaaacg gagagggaga gatagcatat ccacggagag
     9541 ccacgccgga acatatcaca acaggggaca gacaacgcac gggataaaag ggacgttttc
     9601 gtcagaatgt tgttctgccg gcgctgcatg ttctccccgt attctggcct gtgtgatgcc
     9661 agtgtaaatt catgtcaaaa agtgtcagcg agaaaattta cacctgaaaa agtctgtgtc
     9721 atgaaacagt tttatgacaa gacatcaaaa aaatgtcaaa aacgtgatct ttcttcagct
     9781 tttcagaata ttgagtacca ggaagtcaat atcaattctg ggacacatat gcatttacca
     9841 ttaaaaccgg gcacaacaag ctgttcagtc tcaatccata ccccgtgttt tatcaatatc
     9901 agaataagaa tatctgcgga taaataaaaa aggaatgtcg agggagctta ccgcagaggg
     9961 ctctccacct cacaaaccat ttacaaaaat gataccggca tatctttggt ccctgtcaca
    10021 accagacatt gacaatccaa tataacccca aatatatttc gggtaagaag tctgggattt
    10081 atacagatat actgtct
//
LOCUS       DAZGCS010000148        10019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGCS010000148 DAZGCS010000000
VERSION     DAZGCS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.150"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..81
                     /locus_tag="ACHK01_004382"
     tRNA            6..81
                     /locus_tag="ACHK01_004382"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHK01_004383"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHK01_004383"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACHK01_004384"
     CDS             complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACHK01_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO8099215.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACHK01_004385"
     CDS             complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACHK01_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8099216.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACHK01_004386"
     CDS             complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACHK01_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8099217.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="ACHK01_004387"
     CDS             complement(3596..4621)
                     /locus_tag="ACHK01_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8099218.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="ACHK01_004388"
     CDS             complement(5052..5273)
                     /locus_tag="ACHK01_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099219.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACHK01_004389"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACHK01_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO8099220.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACHK01_004390"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACHK01_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO8099221.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2656 a         2584 c         2416 g         2363 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacat aaggctcagt cggaagactg ggccttttgt tttatttgat gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggaacggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca
      601 aaccccatct catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca
      781 gaaatttgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgccgga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gtgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggcagc gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa
     1681 aggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggat agttttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcctaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcgc gaaccgtagg
     3481 gttggcaaaa gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgta gcgacgcccg ttacctcaca ccgacttaac
     3601 gcacgggcgg cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcgaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccagcttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt
     3841 tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa tagtcggcaa
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cagtaaatgc cattcccatc ccggcgtcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ttcccctgac tgtaaagccg tgaagcgttc ttttgctgtg agtggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4441 caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcccg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagccgct
     5101 gccccgccgg caatagcccc agccgtttcc cagttatcct gagtcgtgcc tttcatacag
     5161 gtattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatattgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agtttttaaa gagtgagtgg aagaatatct
     5461 tattaatttt tatgggtttc actgaaaata ataaaggcac ccgaaagtgc ctttatgttt
     5521 ctgatttatc ctttaaagca acggcggatc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattccgccc attaccccga tacccactgc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatta gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgtcgccg ccagcagcac gccgacaatc cctaacggca cgaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagag cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgccagcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata cacctttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccaacgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca taccaagcaa ctggttacgg
     6541 gcctgggtta gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atattgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccaa attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcatcacca
     6721 ttacgctctt cccacggttt cagactgacg aaggccatac cggcattttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggta
     7081 gtattaaacc agccgaagaa accgccctta ttttcgtgat gctcagcaga gacgggttta
     7141 agcagcgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctggatt
     7321 tgcgacattg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg cgaggatggc aaacgtccct
     7501 aataacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcaaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaact cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggttactttg ccgaattctt ccggattttt taaccgcgtc
     7921 tgagcaataa ttgaggcgtt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgaacctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc gttcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcgcc cggatagttt gctgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg taattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc gcgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacgg cacgtagggt tatggagccg gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gtacatcgct gccttcagtg aaattacgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttcggctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgttttcac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaga
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagtttg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt atgaatactg gcgtctcaag gcacataaac acaaaaaaga
     9961 ttaatattct actgttttat tttgacgcgg gttgaaagag gcagaattaa aacctcgta
//
LOCUS       DAZGCS010000149        10018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGCS010000149 DAZGCS010000000
VERSION     DAZGCS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.151"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..312
                     /gene="hokA"
                     /locus_tag="ACHK01_004391"
     CDS             160..312
                     /gene="hokA"
                     /locus_tag="ACHK01_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO8099222.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(301..360)
                     /locus_tag="ACHK01_004392"
     CDS             complement(301..360)
                     /locus_tag="ACHK01_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099223.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(500..712)
                     /gene="cspA"
                     /locus_tag="ACHK01_004393"
     CDS             complement(500..712)
                     /gene="cspA"
                     /locus_tag="ACHK01_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO8099224.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(993..1283)
                     /gene="yiaG"
                     /locus_tag="ACHK01_004394"
     CDS             complement(993..1283)
                     /gene="yiaG"
                     /locus_tag="ACHK01_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO8099225.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1717..2427
                     /gene="yiaF"
                     /locus_tag="ACHK01_004395"
     CDS             1717..2427
                     /gene="yiaF"
                     /locus_tag="ACHK01_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO8099226.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2477..3451)
                     /gene="ghrB"
                     /locus_tag="ACHK01_004396"
     CDS             complement(2477..3451)
                     /gene="ghrB"
                     /locus_tag="ACHK01_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO8099227.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3555..4214)
                     /gene="yiaD"
                     /locus_tag="ACHK01_004397"
     CDS             complement(3555..4214)
                     /gene="yiaD"
                     /locus_tag="ACHK01_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO8099228.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4367..6700
                     /gene="bisC"
                     /locus_tag="ACHK01_004398"
     CDS             4367..6700
                     /gene="bisC"
                     /locus_tag="ACHK01_004398"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO8099229.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6669..7109)
                     /gene="yiaC"
                     /locus_tag="ACHK01_004399"
     CDS             complement(6669..7109)
                     /gene="yiaC"
                     /locus_tag="ACHK01_004399"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8099230.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7106..7669)
                     /gene="tag"
                     /locus_tag="ACHK01_004400"
     CDS             complement(7106..7669)
                     /gene="tag"
                     /locus_tag="ACHK01_004400"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO8099231.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7827..8525
                     /gene="yhjY"
                     /locus_tag="ACHK01_004401"
     CDS             7827..8525
                     /gene="yhjY"
                     /locus_tag="ACHK01_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8099232.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8754..9956
                     /gene="yhjX"
                     /locus_tag="ACHK01_004402"
     CDS             8754..9956
                     /gene="yhjX"
                     /locus_tag="ACHK01_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8099233.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2386 a         2436 c         2585 g         2611 t
ORIGIN      
        1 gaatagccac gggtgcttga gactgtttgt ctcaggtatt caccgaaagg cagacagaga
       61 aaagccccac ctgactataa atcaaagtga ggctacccta tgcctgaaca ccataaggtt
      121 agcctcttac tcgttggaaa tcaacacagg gggctgggaa tgccgcagaa atatagatta
      181 ctttctttaa tagtgatttg tttcacgctt ttatttttca cctggatgat aagagattca
      241 ctgtgtgaat tgcatattaa acaggggagt tatgagctgg cggcattttt agcctgcaat
      301 ttaaaagagt aagagtcttc ggcgggaagt tattcccgcc tttcttacgg cgttgcgcat
      361 tctcattgca cccaaattta tttttcacaa aaataatact agattttatt acgcgatcga
      421 ttatttattt cctgaaaaac aaataaaaaa atccccgcca aatggcaggg atcttagatt
      481 ctgtgctttt aagcagagat tacaggctgg ttacgttacc agctgccggg cctttagcgc
      541 cgctttcgat ggtgaaggac actttctgac cttcgtccag agatttgtaa ccatcgttct
      601 ggatagcaga gaagtgtacg aacacgtctt tagagccatc gtcaggagtg atgaagccga
      661 agcctttgtc agcgttgaac cattttacga taccagtcat tttaccggac atagtgtatt
      721 acctttaata attaagtgtg cctttcggcg atatggcgtg ctttacagat tttgaagcgt
      781 taaaggaatg tgcactacga ggggtatcaa cgataactct tgaagggact tgccttacta
      841 cactgcttta atggtctgta cgtcaaaccg ttgatgtaca ttaagccacg cattggcggg
      901 tgatgcaaca attatttttc atatttatga ttaatcggtt ggaaaacggt cctgtcatca
      961 ggaccgtaaa cagcaataaa gtggataaaa gtctattcca tcaactgctt acttaatgcc
     1021 gggttggctt gaatcaaacg catcaatttt agttcggcac ttgaaggctt cacgcgtctg
     1081 gattcccatt cctttaccat ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt
     1141 tttaaccctg tcccttttcg taactgctca atttcggtac aggacgttgt tctgtgcgtc
     1201 agggtaattt tctgcgtttc atctttaaaa acaatctgtt ccaggctgct caacagctca
     1261 tgcattggat ctttatattc cattgaaaac tcctcaaatc acactgcggg atcgtgaatt
     1321 gcatcgaagc tcattaagaa tagtcgggaa aagcagacac agcggggtga agtcagggca
     1381 tgctctggct atgattcgat tcagcctgtc atttagcgtg acttatagcc acctaacgat
     1441 ctgataaatg ccgatatatc tcttaccact cctgttttgt aaagatttcg aaaatctaac
     1501 cttctgcgaa ggcgtaaatc tctggctaca aaaaaggcaa cggcactatc attttttaac
     1561 aatgaaacaa caaattaacc ttgtttcatt ttctccatga ttttaccagg aagattacgt
     1621 agaaagggta tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt
     1681 cgttgtgcgc tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct
     1741 cgctggtttg cgcctcttgc ggcgttatta atggtagtta gcctgagtgg gtgttttgat
     1801 aaagaaggcg atcagcgtaa agcgtttatc gacttcctgc agaatacagt gatgcgtagc
     1861 ggtgaacgtc taccaaccct gactgccgat cagaaaaaac agtttggtcc ttttgtctct
     1921 gattacgcga ttctgtatgg ttattctcag caggtgaatc aggcgatgga ttccggtctg
     1981 cgcccggtcg tagacagcgt taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc
     2041 ggtccgctgc gtgaaatgaa cggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg
     2101 aagttgcagg ccgatgctgc gcactctgcg ctaaaacaga ccgatgattt gaaaccggtc
     2161 tttgatcagg cattcaccaa agtggtaacg acgccagctg atgcattgca accgttgatt
     2221 ccggctgcac aaactttcac gcaacaactg gtgatggttg gagactacat tgctcagcaa
     2281 ggtactcagg tgagctttgt cgcaaatggc atccagttcc cgacttcaca gcaggcaagt
     2341 gaatataaca aactgattgc gccattaccg gcacaacatc aggcgtttaa tcaggcctgg
     2401 actacagcag ttactgcaac ccaataaaga gtaaaagccc gagcgattat cgggctttac
     2461 ttcacgcagt cgcggcttag tccgcgacgt gcggattcac acagttcttc tcaacctttc
     2521 cttgtaacgc atcaatcaaa ttatccacgg cacaggcggc catgccataa cgcgtctcat
     2581 gggtggcaga tccaatatgc ggtactgcga cgacgttggc cattgagagc aacggcgaat
     2641 ctacggacag tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct
     2701 gcaatgcagc aatcagtgca ttttcatcaa ccaccgggcc acgtccggca ttaatgaaaa
     2761 tggcggagga tttcattttg gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag
     2821 ttaacggcag gatcaggcaa acgaaatctg actcttgtaa cagagtatcc aaatcgcagt
     2881 agcgggcgtt gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg
     2941 gcatgttgaa gccaaagtgc gcacgttgtg ccagcgccat accgatccgt cccatcccga
     3001 caatgcccag tgttttatgg tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg
     3061 tccattcgcc tgcttttacc cgttctgcta cctccacaac ccgacgagcg gtagacaaca
     3121 ccagcgccat cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg tgcatcagca
     3181 gaatttttcg ggcggtaagc gcatcgacat caaaattgtc atagccgacg gagatcgttg
     3241 atgtggcacg cagtttcggc attttttcca gcaatgcggc atcaacattc tcgtttgaac
     3301 ccagtaaacc ttcagcttcg gcaaaaattg ctgcattttg ttcgacggtt tgtgggctga
     3361 ggtttgccac ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa tcatcaggta
     3421 aggctttgta gaggataacg gacggcttca tgcttctctc cattcactga taaggcgatc
     3481 accttagcct gaaatatgat gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc
     3541 tggcatgaaa gggattacag cgggcttaag gtaatttcta cacggcggtt ttgcgcctta
     3601 ccttctgcgg tgctgttgct ggcgattggg ttagccgggc caaggccctg agtacggatg
     3661 cggctggcgt ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc acgttgctgg
     3721 gagagacgca tgttcaggtc gtgaccaccc gtgctgtcgg tataaccaat cacgttaacc
     3781 gccgttttcg gatactcttt cagcaccatt gccacgccgg tcagggtgtt agcgcccgca
     3841 ggtttcaggg tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt gaggataatg
     3901 ttatccccgc tgcgggttac gctaacacca gtgccgcgca ttttgtcacg cagcttcgct
     3961 tcctgcacat ccatgtaata acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca
     4021 atcagcgcgc ctttaccgcg atctttcttc gaagaagaga gcgcaccaat acccgcgccc
     4081 acgagagagc ccagacctgc gccgatagca gatttacctg cttcgcgttc gccggtgtaa
     4141 gggttagttg tgcagccaga taccgccaga gcgccactca ctacggcggc aataagataa
     4201 acacgtttct tcattgttaa tccttaataa cctttttatt ctttgccacg ggttccgtgg
     4261 cgggagatta tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag
     4321 taatatttaa ctgctcaata catctaacct ttcaggagtt ttcggtttgg ccaactcatc
     4381 ctcacgatat tccgttctga ctgccgccca ttgggggccc atgctggttg aaaccgacgg
     4441 cgaaaccgtg tttagctcgc atggcgcgtt agccacagga atggaaaact ccttgcagag
     4501 cgcggttcgc gaccaggttc acagcaatac acgggtacga tttccaatgg tgcgcaaagg
     4561 ctttcttgcg tcaccggaaa atccgcaagg cattcgtggg caggatgaat ttgttcgcgt
     4621 gagttgggat gaggcgctgg atcttattca ccaacaacat aaacgcattc gtgaggctta
     4681 tggtccggca tcgatttttg ctggttccta cggctggcgt tcaaacggcg tgctgcataa
     4741 ggcctcgaca ttattacaac gctatatggc gctggcaggc ggttataccg ggcatctggg
     4801 ggattattcg accggcgcgg cacaggcgat catgccgtat gtcgtgggtg gtagcgaagt
     4861 ttatcaacag caaaccagtt ggccgctggt gctggaacat agcgatgtcg tggtgttgtg
     4921 gagtgctaac ccactcaata cgctgaaaat tgcgtggaat gcatctgatg agcaggggct
     4981 ttcttacttt tccgcactgc gtgacagcgg gaaaaagctg atctgtattg atccaatgcg
     5041 atcggaaacc gtcgatttct ttggcgataa aatggagtgg gtggcaccgc acatgggcac
     5101 cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt ggcacgacga
     5161 agcgtttctg acgcgttgca ccacaggtta tgccgtcttc gcctcttatt tgctgggcga
     5221 gagtgacgga atagcgaaaa acgccgaatg ggcggcagag atttgtgggg ttaacgcagc
     5281 gaaaatccgc gagctggcgg ctcttttcca ccaaaacacg accatgctga tggctggctg
     5341 gggaatgcag cgccaacagt ttggtgagca aaaacactgg atgatcgtca cgctggcggc
     5401 aatgttgggg caaatcggca cacccggcgg cggttttggt ctttcttatc attttgccaa
     5461 tggtggtaac cccacgcggc gttctgcggt gctctcttcc atgcagggca gcttgccggg
     5521 tggctgcgat gcggtggata aaatccctgt tgcccgcatt gttgaagcac tggaaaaccc
     5581 tggtggcgca tatcaacaca acggtatgaa ccgacatttc ccggatattc gttttatctg
     5641 gtgggcgggc ggtgccaact ttactcatca tcaggatacc aatcgtctga tccgtgcctg
     5701 gcaaaaaccg gagctggtgg tgatctctga atgcttctgg acggcggcgg cgaaacacgc
     5761 ggatatcgtt ctgcctgcga ctacctcttt tgagcgtaat gatctcacca tgaccggtga
     5821 ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg ccgccacgct atgaagcgcg
     5881 taatgatttt gatgtttttg ccgagttgag tgaacgttgg gagaagggcg gctatgcgcg
     5941 gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg ttttataacg ttgcccggca
     6001 gcgcggggca agccagcagg ttgaattgcc gccatttgct gagttctggc aagccaacca
     6061 gttaattgag atgccggaaa acccggacag cgagcggttt attcgcttcg ctgatttttg
     6121 ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc aagattgaaa tcttctcaca
     6181 gcgtattgcc gattacggtt acccggattg ccctgggcat ccaatgtggc tggagccgga
     6241 cgaatggcag ggcaatgccg aaccagaaca gttgcaggta ctttctgccc atccggcgca
     6301 ccgcctgcac agccagctga attacagttc tctgcgcgaa ttgtacgcgg tagcaaaccg
     6361 tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc ggcataaccg aaggggatat
     6421 ggtgcgggtg tggaactccc gtgggcagat ccttgccgga gcggtcatta gcgagggaat
     6481 taaacctggc gtgatttgca ttcacgaagg ggcatggccg gatctggatt taaccgctga
     6541 cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa gatctcccca gctcgcggct
     6601 ggggaatggc tgtgcgggta atacggcgct ggcatggctg gaaaaataca acggtccgga
     6661 actgacactt acagcgtttg aaccaccggc cagctcataa tccaggtggg tagttgggtt
     6721 tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg cacggtaaaa atctatcgcc
     6781 ggttgatttt tttgataaac ctccagcatc agatggggat agcgctgctg cacatactgc
     6841 atcagcgcct taccaatacc gcgtctgacg gccttcggtg cgacaaacat tgctgccaga
     6901 aatcggcctt ccataatgct gacaaaaccg agaagcttac cgtcctcttc ccagacccag
     6961 ttttgcgcgt tggcaagata ggcatcccgc accagcggaa tgcagtcacg ccagtaattc
     7021 gcttttataa agggatgccc ccaggttgta ctttccagcc acagttcgag gatcgcgggg
     7081 agttctgaac gttgcgcttc ccgaatcatg gtttatttcc cggatagcaa cagcagccaa
     7141 ccacatgatc attcaccagc ccacatgcct gcataaagga gtaacagatt gtggtgccga
     7201 caaacttaaa accacgtttt ttcagtgcct tagatagggc gtcggaggcg gacgtagatg
     7261 tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg ctgatgattt acaaacgacc
     7321 agacaaagtc ggcaaacggt tcgccgttct gttccatttg caggtacgcc cgcgcattac
     7381 caataattgc ctgaattttc cctcgatggc ggataatccc ggcgtcctgt accagtcttt
     7441 cgacatcctc ttcctgcatt gctgcgacct tcaccggatc gaactgatga aagcaggcgc
     7501 gatagttttc gcgttttttg aggacggtga tccacgataa cccagcctgc tgcccttcaa
     7561 ggcagatcat ttcgaacagt tttttactgt cagtttcagg cacgccccat tcattatcat
     7621 ggtaggcaat ataaagcggg tcctgactca cccagccgca acgttccata ctttccctcg
     7681 ctatgtaccc gatcgtaaaa atttcagccg cttttcttga cgcatcggtg aaaaagtcaa
     7741 tatttaatac agggctacta tgcccgatat ccttcatgca atgacaataa tattgccgaa
     7801 ttcggctctt ccctggagtc gtgttaatga tcataaaaaa aagcggtggt cgctggcagc
     7861 taagcctgct ggcgagcgtg gtaatcagtg ccttttttct caacacagct tacgcctggc
     7921 aacaagaata tatcgttgat acgcaacccg gacattccac agagcgttac acctgggata
     7981 gtgatcatca acctgattac aacgatattt tgtcgcaacg tattcaaagt agccaaaggg
     8041 cgctgggact ggaagtcaat ctggcggaag aaactcctgt ggatgtgacc agcagtatga
     8101 gtatgggctg gaattttcct ttatatgaac aggttacaac cggcccggtc gcggcattac
     8161 attacgatgg cacaaccacc tcgatgtata acgagtttgg cgacagtact accacgctga
     8221 ccgatccgtt atggcatgcc agcgtgagta gcttaggctg gcgtgttgac tcccggcttg
     8281 gcgatctccg accctgggcg caaatcagct ataaccagca atttggcgag aatatctgga
     8341 aggcgcaatc aggcctgagc cggatgacgg cgacaaacca gaacggcaac tggctggatg
     8401 tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc cgcctatgcc gcgctaactc
     8461 aggcagaaaa taccactaat aatagcgact atctgtatac gatgggggtt agcgccagat
     8521 tttaacgtaa cagtcacaat tgaaaccatt gaataacaac agttgtggcg atagtgggtg
     8581 ctaacttacc aaataataaa tttggtgaat aattgtcgcg tcattcattc ctgaactaag
     8641 gcatttcatt ccgttctgat ggcatttcat gccgtttttc cccaggcata aaatgcactt
     8701 cgttatggtt gtcggcagag atttttcctt tttattactg caggaatact gccatgacac
     8761 cttcaaatta tcagcgtacc cgctggctga cactcatcgg tactatcatt acccagtttg
     8821 cactggggtc ggtttatacc tggagcctgt ttaatggcgc gctttctgcc aagctggatg
     8881 cgccggtaag ccaggtcgct ttctctttcg gcttgttaag tctggggctg gcaatttcgt
     8941 cttctgttgc gggcaaattg caggaacgtt ttggcgttaa acgcgtcacc atggcttccg
     9001 gcattttgct gggattaggc ttcttcctga ccgcgcattc caacaacctg atgatgctgt
     9061 ggttaagcgc cggtgtgctg gtcggtctag cggatggcgc gggttacctg ctgacgctct
     9121 ctaactgcgt gaagtggttc ccggagcgta aaggtctgat atccgcgttc gctatcggtt
     9181 cttatggtct gggtagcctg ggtttcaaat ttatcgacac gcagctgctg gaaacggtgg
     9241 gtctggaaaa aacctttgtg atttggggag cgattgcgct ggtgatgatt gttttcggcg
     9301 caacgttaat gaaagacgca ccaaaacagg aagtgaaaac cagcaatggc gtagtggaga
     9361 aagattacac gctggcagag tcgatgcgta aaccgcagta ctggatgtta gcggtaatgt
     9421 tcctgactgc ctgcatgagc ggcctgtacg tgattggggt agcgaaagat atcgcccaaa
     9481 gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt aacggttatt tccatcgcta
     9541 acctttcagg tcgtctggtg ctgggcattc tgtctgacaa aattgcccgt atccgtgtta
     9601 tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc cctgctgttt gcaccattaa
     9661 atgcagtgac gttctttgca gcgattgcct gcgtggcatt taactttggc ggcactatta
     9721 ccgtctttcc atcactggtc agtgagttct ttggcctcaa taacctggcg aaaaactacg
     9781 gtgtgattta cctcggtttc ggtatcggta gcatttgtgg ttcgattatc gcctcactgt
     9841 ttggcggctt ctatgtgacc ttctacgtga ttttcgccct gctgattctg tcattggcgc
     9901 tttctacgac aattcgtcag ccagagcaga aagtattgcg tgaggcgcat atttagaaat
     9961 atgttgctac tgaaataaat gcgaggaggg agacttcaaa gtctcccttt ttttatgg
//
LOCUS       DAZGCS010000150         9986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGCS010000150 DAZGCS010000000
VERSION     DAZGCS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.152"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..611)
                     /gene="yncG"
                     /locus_tag="ACHK01_004403"
     CDS             complement(<1..611)
                     /gene="yncG"
                     /locus_tag="ACHK01_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO8099234.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNE"
     gene            878..2377
                     /gene="ansP"
                     /locus_tag="ACHK01_004404"
     CDS             878..2377
                     /gene="ansP"
                     /locus_tag="ACHK01_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO8099235.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2490..3551)
                     /gene="yncE"
                     /locus_tag="ACHK01_004405"
     CDS             complement(2490..3551)
                     /gene="yncE"
                     /locus_tag="ACHK01_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO8099236.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3793..5895
                     /gene="pqqU"
                     /locus_tag="ACHK01_004406"
     CDS             3793..5895
                     /gene="pqqU"
                     /locus_tag="ACHK01_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO8099237.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATSRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5931..6596)
                     /gene="mcbR"
                     /locus_tag="ACHK01_004407"
     CDS             complement(5931..6596)
                     /gene="mcbR"
                     /locus_tag="ACHK01_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO8099238.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6794..7831)
                     /gene="curA"
                     /locus_tag="ACHK01_004408"
     CDS             complement(6794..7831)
                     /gene="curA"
                     /locus_tag="ACHK01_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO8099239.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8012..8530
                     /gene="mddA"
                     /locus_tag="ACHK01_004409"
     CDS             8012..8530
                     /gene="mddA"
                     /locus_tag="ACHK01_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO8099240.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8527..8976
                     /gene="ydcZ"
                     /locus_tag="ACHK01_004410"
     CDS             8527..8976
                     /gene="ydcZ"
                     /locus_tag="ACHK01_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8099241.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8977..9210)
                     /gene="ydcY"
                     /locus_tag="ACHK01_004411"
     CDS             complement(8977..9210)
                     /gene="ydcY"
                     /locus_tag="ACHK01_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO8099242.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9296..9469)
                     /gene="ortT"
                     /locus_tag="ACHK01_004412"
     CDS             complement(9296..9469)
                     /gene="ortT"
                     /locus_tag="ACHK01_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO8099243.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9664..9759
                     /gene="yncL"
                     /locus_tag="ACHK01_004413"
     CDS             9664..9759
                     /gene="yncL"
                     /locus_tag="ACHK01_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO8099244.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2406 a         2438 c         2441 g         2701 t
ORIGIN      
        1 atttcattcc tttttaatac ttcttgtaat ttcggtaact gacatacagc atcagcaatc
       61 gcactgatat tcgtggcatt atcctgaaac cattcatgcc caggccccca tgtgcgcata
      121 gtgcaaagat aacagtccac cagcgttagt tgctcaccaa acgcataagg ttcagcggtc
      181 aattgtgaat tcagccagat ataaagcgat ttacgatatt caataacatt cttctttaac
      241 tgttcaggtg cgtcaggagc ccaacgctca gggtaatcgg cgaaagtgaa tgttggatag
      301 acattagcaa ccagccagac caatagccgt tgaaactgct gacgttcggc gcgcccaacc
      361 ggtggcgcaa ggtccgggcg acgatcgaga accatcaatg caatcgccgc tgtctccgtc
      421 ataatctcgt cattttccag cgccagtgtc ggaatctgac acaacgggtt cagggttttc
      481 aataactcgc gtgaagttcc ctcgtggtca aaaccactga catcaacgaa ctgataagga
      541 atatcagcca gtgttagcat cagctcactg attgtagagc cccatccggg tacgccatac
      601 accttaatca tatgcccctc acaatctttt aaagattaag tgtaggacag tacacaggtt
      661 aaccagcaaa aggatttgtc attttttcgt cagttgtatt aatccccttc aggattgtaa
      721 ggggatgttt tgcatcgttt atcgataacg ggtaggatgc tgcgccgata gttttatacc
      781 cataaagaat aatggtgata actatcatcg ccaggatgaa taaacattgt tcatggcaac
      841 ttatatgact ttttcattaa agcaatcagg gagagcaatg agtaaacacg acaccgacac
      901 ttcagatcaa cacgccgcga aacgccgctg gcttaatgcc cacgaagagg ggtatcacaa
      961 agcgatgggc aatcgccagg tacagatgat cgccattggc ggcgcgattg gcaccggctt
     1021 gtttttaggt gcaggagccc gactgcaaat ggcgggtcca gcactggcac tggtttattt
     1081 aatttgtggc ttgttttcgt tttttattct gcgtgcattg ggtgagctgg tgctacaccg
     1141 cccttccagt ggcagttttg tctcttatgc ccgtgagttt ttgggtgaga aagccgctta
     1201 tgttgctggc tggatgtact tcatcaactg ggcgatgacg gggattgttg atattaccgc
     1261 cgtcgctctg tatatgcatt actggggtgc gtttggcggc gtgccgcagt gggtctttgc
     1321 gctcgctgcg ctgaccatcg ttggcaccat gaatatgatc ggtgtgaaat ggtttgcgga
     1381 gatggagttc tggtttgcgc ttattaaagt gctcgccatt gtgacctttt tggtcgtggg
     1441 tacagtgttc ctcggtagcg gtcagccgct ggatggcaac accactggct ttcatttaat
     1501 caccgataat ggcggcttct tcccccacgg tttgctgcct gcgctggtgt tgattcaggg
     1561 cgtagtgttt gcttttgcct ccattgaaat ggtgggtaca gctgccggag aatgtaaaga
     1621 tccgcagacc atggtgccta aagcgattaa cagtgtgatt tggcgtattg gcctgtttta
     1681 cgtcggctcc gtggtgttgc tggttatgtt attgccgtgg agcgcgtatc aggcggggca
     1741 aagtccgttc gtgacgtttt tctctaaact gggtgtgcca tatatcggca gcattatgaa
     1801 cattgtggtg ctgaccgctg ccctctccag cctgaactca ggcctgtact gcaccgggcg
     1861 tattctgcgc tcgatggcga tgggcggttc cgcaccgagt tttatggcga aaatgagtcg
     1921 tcagcatgtg ccgtatgccg ggattctggc gacactggtt gtgtatgtcg tcggcgtatt
     1981 cctcaactat ctggtgccgt cgcgcgtatt tgagattgtg ttgaacttcg cgtcgctggg
     2041 aatcatcgct tcatgggcgt ttatcatcgt gtgccagatg cgcctgcgta aagcaattaa
     2101 acaaggcaaa gcagcggatg tcagttttaa actgcctggc gcgcccttca cttcctggct
     2161 gacattactg tttttactga gtgtcctggt gctgatggcg ttcgattacc cgaacgggac
     2221 ttatactatc gcggcgctgc cgattatcgg tattctgctg gttataggct ggtttggtgt
     2281 gcgcaaacgc gttgctgaaa ttcacagcac tgcgccagtc gtcgaggaag atgaagaaaa
     2341 acaggaaatt gtgtttaagc ctgaaacggc gagttaattg caaaattgcc cggctacgtg
     2401 ccgggcaaat tcagattact gacgaaggtg ccggatgcgg cgtgataaaa gtcaggcaag
     2461 tctctccgtt ttcaccctcg ccatatctat tacagcgcaa tacgaatcac atcgtctggc
     2521 tgggtagctt cctgctgttt agtggatttt tgtttcacac tgacatacag cgttttgcca
     2581 tcggcagaca gcgccaggct gtttggatga gtcggcgtat cgaacgtttt caccacttta
     2641 tagcttttcg cgtcaatcac actaacttta cctgcctgac gatgcgttac gtaggcttca
     2701 ttacgcgctg ggttaaacag cacagccagt gattccggtg ccgcaacctt cgccagaata
     2761 ttgccattac gggtatcgac cactaacact tctgcggctt tagaatcggt gataaatgca
     2821 cgctgcctgg cggtatcaag gctaatgttg ataaagaagt gctctttgcc gtcatccagc
     2881 agctttttac ggctgaggat tttattgtcg gcggtgtcga tggtaatcaa ttcgccgtca
     2941 gcgttagtgg tgtaaagacg tttgcctttg ctatccagcg ccagaccggt actcatttta
     3001 ccggtgttct ggatggcggt tttcagtttg atattctcgc catcaacgac ccaaatcacg
     3061 ctatctttac caataccact gatgtaaacg gtgttcgtgg catcatcagc taccagctca
     3121 cgcggttgca gcgggcgcac ctcttccgtg cgcttacgat catccagcac cagacggcct
     3181 ttcacttcgc ccgttttggc atctatcgcc gtgaccgcgc tgtttacggt gttaccaaac
     3241 cacaacgtct gagtcgtgtt attgatggtg gcaccaaacg gcttgagatc gttatggatc
     3301 gcctgcgtca cttccagagt gaccggatca agacgataaa ccacgccacc tttatccagt
     3361 ttgcggcttt gcgaagtggc gagccacagc gcgttttctt gctggctata agccatttcg
     3421 taggcacctt tacctaccgc tttacgcagc atttcttctg cggcctgcgt actgaatgat
     3481 gaagcaacaa gcaatgaacc taacagtaat gaaccacgca ggcgcgatga aaacagatga
     3541 cgtaaatgca tgacgactcc ctttgatgaa ccaatatttt tgctgcttca caatgcttcg
     3601 ctaagcgtaa tcatcgttac gctcttgtta taaatgggaa tggtaatcat tattttctca
     3661 aatgtggagc atttcttgcc gttacgacta caattttctc aattaattaa cgtatcgtgc
     3721 cgtttcgttt tccataaatt tacaatttta ttaccatgac agcgttttcc cattcttaac
     3781 ctggcttttt tcatgaagat tttttccgtc cgacagaccg ttttgcccgc actgcttgtc
     3841 ctttcccccg ttgtttttgc cgctgatgaa cagactatga ttgtcagtgc cgcaccgcag
     3901 gtggtttcag aactggatac cccagcagca gtaagcgtgg tggatggcga ggagatgcgc
     3961 ctggcaacat cgcgcattaa cttgtccgaa tcactgaccg gcgtgcctgg tttgcaggta
     4021 caaaaccggc agaactatgc gcaagattta cagctgtcga ttcgcggatt tggctcccgc
     4081 tccacttacg gtattcgcgg tattcgcctg tatgtggacg gtattcccgc caccatgccc
     4141 gacgggcaag ggcaaacatc caacatcgat ttaagcagtg tgcaaaatgt ggaagtgctg
     4201 cgtggcccct tctctgccct gtatggcaac gcgtctggtg gggtaatgaa tgtcaccacc
     4261 cagaccggac aacagccacc aaccattgaa gccagtagtt actacggcag ttttggcagc
     4321 tggcgctatg ggctgaaagc aacgggcgca acgggagacg gcacacagcc tggcgatgtc
     4381 gattacaccg tctcaaccac gcgttttacg acccacggct atcgtgacca tagtggcgca
     4441 cagaaaaatt tagctaatgc caaactgggc gtacgcattg atgaagccag caaattaagt
     4501 ctgattttca atagtgtgga tatcaaagca gatgacccag gtgggctaac caaagcagaa
     4561 tggaaggcta atccacaaca agcgcctcgt gcagaacagt acgatacgcg aaaaaccatc
     4621 aagcaaactc aggctgggtt gcgctatgag cgtagcctga gcgcgcaaga tgatatgagt
     4681 gtgatgatgt atgccggaga gcgagaaacg acccagtacc agtcaatacc catggcacca
     4741 caacttaacc cgtcacatgc gggcggcgtg attaccctgc aacgccatta ccaggggata
     4801 gacagccgct ggacacaccg tggtgaactg ggcgttccgg tcacgttcac taccggcctg
     4861 aactacgaaa acatgagtga aaaccgcaag ggctacaata acttccgcct gaatagcggc
     4921 atgccggagt acgggcaaaa aggtgagttg cgtcgcgacg aacgcaatct gatgtggaac
     4981 atcgatccct atttacagac gcagtggcag ctgagcgaaa aactgtcgct ggatgctggc
     5041 gtgcgctaca gctccgtgtg gtttgattcc aacgaccatt acgttactcc gggtaacggc
     5101 gatgacagcg gtgatgccag ttatcataaa tggctacctg ccggttcgtt aaaatatgca
     5161 atgaccgatg cctggaatat ctatctggca gccgggcgag gttttgaaac gccgacgatt
     5221 aatgagctgt cttatcgtgc tgatgggcaa agcggtatga actttggttt aaaaccatcc
     5281 accaacgata caattgagat cggcagtaaa acgcgtattg gtgatgggct gcttagtctc
     5341 gcattgtttc agaccgacac tgatgatgaa attgttgtcg atagcagtag cggtgggcgt
     5401 acgacttaca aaaatgccgg aaagacccgt cgtcaaggcg ctgaactggc atgggatcaa
     5461 cgtttcgcag gagattttcg cgtaaacgcg tcctggacct ggcttgatgc gacctatcgc
     5521 agcaatgttt gcaatgaaca ggattgtaac ggtaatcgga tgccagggat cgcccgtaat
     5581 atgggctttg cgtcgatagg ttatgtaccg gaagatggtt ggtatgcagg cacggaagcg
     5641 cgttatatgg gcgatattat ggcagatgat gaaaatacgg caaaagcgcc gtcttatact
     5701 ctcgtcggct tattcaccgg gtataaatac aattaccaca atttaactgt ggatttattt
     5761 ggtcgtgtcg ataatttatt cgataaagaa tacgttggtt ctgtcattgt caatgagtca
     5821 aacgggcgat attacgaacc ttcgcccgga cgaaattatg gtgtcggcat gaatattgcg
     5881 tggagatttg agtaattacc ttgatgcccg gtatttgccg ggcatttact ttaacgattg
     5941 tattgctggt ataaaatagc aatattttgt tgcataattt cagcaagaca atgtctgcat
     6001 ccctctttgt cttttgcttt taacgcagcg agtaacagat gatagttttc tatatgctcc
     6061 cgtaattccg ctggattaat cgcttcatag agataatgta aaccaggccc catcctgacc
     6121 cacagttgct caatcatctc acacaggatg ggcatatttg agcgatgata aatcgctaag
     6181 cgaaatagcc tgtttacatt aatgatttgt tccatgtcgc ccttttcttg cgcctgctgt
     6241 aatttctcga gcaattcctg gagttccgcc aggtcttgcg gggtgaggtt ttcaacagcc
     6301 agagcaactg ccattaattc cagttcgtag cggatccgat tgatttcgtc taattgacgt
     6361 ttcctcactt ccggaactgt aaatgcttgt gcaggtgcga cagaaagcgc attcaccgaa
     6421 accagacgta ataatgcttc acgcacaggt gtaatactca tacctaattg ctccgccaga
     6481 tttttagtaa tcaggcgtgc gccaggtttt aatgcgccaa tactaagctg atgtttcagg
     6541 tcgttctcaa cctgcagagt caaactgaca tgtttcattt ttcccattcc aggcatttta
     6601 attccttcgc gtgttttctg tccaaccctg cctgcacggc agaaggagtt cacctggtgt
     6661 attaaagtga ataatcataa accacccacc tttgttgtta ttagcagcag aacaatgacg
     6721 tggttagtta attaaagctg aaaataagct ttttgcctgg atttttatgg cggcttacgc
     6781 cgccgttgaa actttaatca tcacccgcga cgcggatcac cactttgccg aagtttttac
     6841 ccttcagcag gccgataaac gtctgtggcg cattctctaa accatcagta atatcttcgt
     6901 ggtagtggat tttatcctct ttcacccatt gccccatctc cttctgaaac tcatggatgc
     6961 ggtgaccata atcctgagcg ataataaacc cttgcaagcg aatacgtttt ttcagcaccg
     7021 ttgccatcaa caggggtaaa cgatccggac cgggtggtag ctctgtagcg ttatagctgc
     7081 tcactaagcc acaaacggga atgcgcgcag atgtattaag taatggtagc actgcatcaa
     7141 ataccttacc gcccacgttt tcataataga tatcaatacc ttttgggcac gctttcacca
     7201 gttgttcggc aaaatcatcc gcgtggtgat caagacaaac atcgaagccc agcacctcga
     7261 tagcatggcg gcatttttcc gcgccaccgg caacccccac caccctgcaa cctttaagtt
     7321 tgccgatttg ccccaccgtt gctcccacag gacctgtcgc cgcagctacc accaacgttt
     7381 cgccctcttt aggctgaccg atatccagca gccccatata agcggtaaag cctggcatcc
     7441 ccagcacacc cagcgaccac gatggatttt gcggatgatc gccaagtttc accagatcat
     7501 caccactgga tatgtcatag tcctgccatc cactgtagcc cagcacccag tcgccaggct
     7561 gataatcagg atgattcgac tccacaacac ggctcaccgt gccgccgacc atcacgccgc
     7621 caatatcaac aggcggtgaa taagatggct catcgctcat acgtccacgc atatacgggt
     7681 ccagggacaa ataaactgtg cgcagtaaca cctgtccttc acccggtgtg gcgacatcat
     7741 cttcttcaag acggaaattc tccggaaccg gtgcgccatg tggacgcgag gccagaaccc
     7801 aacggcggtt acgctgcttt tgttgcccca taatgctctc ctgtgctttt ggcatgaatc
     7861 catcaatagt agcaggctcg caggattggt tttgtttgca gccgtcacat cccctctgca
     7921 acacgcaata atttcatcga ggaatccgtt gcttcgggct gttttcgata gtattgataa
     7981 catcatcatc attattttat tgaggccaga catgtccatc cgttttgccc gcaaagccga
     8041 ctgtgctgcc attgcggaaa tttataacca cgccgtgttg tatacggcgg ctatctggaa
     8101 tgaccaaacg gtggatgcag ataatcgcat tgcctggttt gaagcgcgga ccatagcagg
     8161 ttatccggtg ctggtaagtg aagaagatag cgtagtgacg ggatatgcct cgtttggcga
     8221 ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat tcggtttatg tccatcccga
     8281 tcatcagggc aaaggtctgg ggcgtaaatt gttaagccga ttgattgatg aagcgcggga
     8341 ttgcgggaag catgtcatgg tcgccgggat cgaatcgcaa aatcaggcct cgctgcatct
     8401 gcaccagtcg ctgggatttg tcgtcaccgc acaaatgccg caggtgggca ctaaatttgg
     8461 gcgctggctg gatctgacat ttatgcagtt gcaactcgac gaacgcactg aaccggacgc
     8521 gattggatga atcagtcgct cacccttgct tttttgattg ccgccggaat tggtctggta
     8581 gtccagaaca cgctgatggt gcgcatcacc cagacctcct ctaccattct catcgccatg
     8641 ttgctgaact cactggtggg gattgtgctc tttgtttcta ttttatggtt taaacaaggc
     8701 atggcggggt ttggcgaact ggtgtccagc gtgcgctggt ggacacttat acccggcttg
     8761 ctgggatcgt tttttgtctt cgccagtatc agcggatatc agaatgtggg agcggcgaca
     8821 actattgcag tactggtcgc cagccagcta attggcggtc tgatgctgga tatcttccgc
     8881 agtcatggcg taccactacg ggcgttgttc ggcccaattt gtggcgcaat tttacttgtg
     8941 gttggcgcct ggttggtagc cagacgctca ttttaatcag aggatggtgc cgcctttggt
     9001 taactgttcg tggcgcgctt ccatatcttc tttatgcttg cgaccgtgat gagcaatagc
     9061 ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga cttaactccg cgtcatcgct
     9121 cagggcgatt aataattcgt tcatatgggc aatgacgctc tcttcaatcg cggcatccac
     9181 gcgcgcgatg acttcatcca gatgcgacat actttctcct taaccacttg tgaaatttat
     9241 cgattcatcc aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa
     9301 acagtgaaaa caacagcgcc accgggaaac tcatcggcca tgttgccccc accagaaagg
     9361 cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga
     9421 ttgctgccat aaccgcataa aaaacaagca tatgttgata gagagacatg tacgggcgtc
     9481 cagccagatg aataaaacgc gcgcaaatat gcgtcatttt ttgacgtgca tcaagttttt
     9541 tcaccagttt gagaaaaaat catcaaacag caacttgccc ttatccatct tcagaatgag
     9601 tattattgcg gattttctta acccgtacta tacacatagg ggcaatgata aaaggtggca
     9661 aaaatgaatg tttccagtag aactgtagta ctgataaatg tctttgctgc tgttggtttg
     9721 tttactctta tctctatgag atttggctgg tttatttgat gtagtgattt tgcacgcagc
     9781 acatactgtc agagaaatca actctctgac agtacgcaag atcttctcat actctccagg
     9841 tttacaacat tatccttaat atttacttct ttttgctggc atctacattg aaatgagtag
     9901 aaaacatccc cctactgtcg tctatacaaa cagcttactg attcaaacgg aatcttgttt
     9961 tcacattttt ctatcatgaa tacttt
//
LOCUS       DAZGCS010000151         9883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGCS010000151 DAZGCS010000000
VERSION     DAZGCS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.153"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..429
                     /locus_tag="ACHK01_004414"
     CDS             <1..429
                     /locus_tag="ACHK01_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8099245.1"
                     /translation="QSLGEVRVPHRGNVVEKVIEGAYEVVGVFDRIEEKRDAMQSLVL
                     PPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGI
                     SGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            429..629
                     /locus_tag="ACHK01_004415"
     CDS             429..629
                     /locus_tag="ACHK01_004415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099246.1"
                     /translation="MKHIPEGMIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            767..1240
                     /locus_tag="ACHK01_004416"
     CDS             767..1240
                     /locus_tag="ACHK01_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8099247.1"
                     /translation="MKTLSQNTTSSVSAPETDLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPESAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1256..1732
                     /gene="radC"
                     /locus_tag="ACHK01_004417"
     CDS             1256..1732
                     /gene="radC"
                     /locus_tag="ACHK01_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8099248.1"
                     /translation="MQQLSFLPGEMTPGERSLIHRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPGKTDRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1801..2022
                     /locus_tag="ACHK01_004418"
     CDS             1801..2022
                     /locus_tag="ACHK01_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8099249.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            2097..2471
                     /locus_tag="ACHK01_004419"
     CDS             2097..2471
                     /locus_tag="ACHK01_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8099250.1"
                     /translation="MSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            2518..2895
                     /locus_tag="ACHK01_004420"
     CDS             2518..2895
                     /locus_tag="ACHK01_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8099251.1"
                     /translation="MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTPSQLINSIDILRARRATG
                     LMTRSHYRTVNDITLGKRTEAKR"
     gene            2892..3380
                     /locus_tag="ACHK01_004421"
     CDS             2892..3380
                     /locus_tag="ACHK01_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8099252.1"
                     /translation="MNLSLTLEADSLNVQALNMGRIVVDVDGIELAELINNVTENGYS
                     VRVVEDSGQQSTCTPPPLTILAGIRCSTAHITETDNAWLYSLSHQSSNFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            3392..3589
                     /locus_tag="ACHK01_004422"
     CDS             3392..3589
                     /locus_tag="ACHK01_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8099253.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            3674..4516
                     /locus_tag="ACHK01_004423"
     CDS             3674..4516
                     /locus_tag="ACHK01_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8099254.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTALDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(5719..7083)
                     /locus_tag="ACHK01_004424"
     CDS             complement(5719..7083)
                     /locus_tag="ACHK01_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspK/GogB family type III secretion system
                     effector"
                     /protein_id="HFO8099255.1"
                     /translation="MLSTSQLKLSGTACHHITETPASFKSEITSRQLEDARESGCLHI
                     KNCDMEFLPHLPEEITAITIENCNKLTGLTGLPVNIKSLSVINCENLKVSELPPTVRE
                     LHIELNNSSFVHTVPEGVEHLKLGHCQVSGVPDSVRSLELTGRAAETLTGVPSGLCSL
                     SIRDYNPEYQVRIDNFISPSLQTLSLTGCSNIVLPEKLPKGMVSATIYTEQKTTWNIV
                     AEGFPEEMDLDLQNVQLSPDVIKEKNITFQGYAMEAALHFRLGDIVYGLSEPRGKVVN
                     TIKLVNDFSKKDILIQNTLTSAVWDYKSPRKYKKDALIKRALSESDRGINFKQYLKNH
                     SQYNVTMADLSVCNRDKLWAKTSKAGLEFQLLVRNKMVHFCADGLVDTLKNIANKGDA
                     YGQSITASELRWIYRNQDNEQIMKNIKFYLRGKEVPAEKILGMPEWKDYHPKRSGLIS
                     EYSK"
     gene            complement(7374..8012)
                     /locus_tag="ACHK01_004425"
     CDS             complement(7374..8012)
                     /locus_tag="ACHK01_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO8099256.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     ISFMDAFSVEPIQGSLLDRLGGRCRRLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLTDQRFCIISGQDVRF"
     gene            8210..8794
                     /locus_tag="ACHK01_004426"
     CDS             8210..8794
                     /locus_tag="ACHK01_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO8099257.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDEQTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKFIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
     gene            complement(9040..9783)
                     /gene="espF"
                     /locus_tag="ACHK01_004427"
     CDS             complement(9040..9783)
                     /gene="espF"
                     /locus_tag="ACHK01_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO8099258.1"
                     /translation="MLNGISQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLAAYEKSKSLEASGLKPARPAPPPPTSGQVSGSSRSLPPIAQALKDHLAAYEKSKSL
                     DTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSLDTSGLKPSRPA
                     PPPPTSGQASEASRPLPPIAQALKDKNRY"
BASE COUNT         2713 a         2464 c         2178 g         2528 t
ORIGIN      
        1 cagtctctgg gggaagtgcg tgttccgcac cggggaaatg tagtggagaa agttatcgaa
       61 ggggcttacg aggtggtggg ggtttttgac cggatagagg agaagcgtga tgccatgcag
      121 tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
      181 tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccacg acgccgggag
      241 gattacggaa aggacctgtg gagtgcttat cagaccatcc aggagaatat gctgaaaggg
      301 gggatttccg gtcgcagtgc cagaggaaaa cgtatccaca cccgtgccat tcacagcatc
      361 gacaccgata ttaagctcaa ccgcgcattg tgggtaatgg cagaaacgct gctggagagt
      421 atgcgctgat gaaacatatc cctgaaggca tgattcaggt accagaatca cctgacgccc
      481 cggaggcgct gccggggtgt cgttccattt ctgatgatga cctgtgcgcc tggtgtaccc
      541 gtctgcttta ccgtcccgga gaactcagtc tgtgcagact gagtacaggc aatggcatct
      601 ggccatccgt ctgtgaccgg aacggttagc gtacggctgt ccggaatttc agccgaatat
      661 cattcatccc tgacattacc gggtctcacg gtcagtgatg aacgtgatgt tgtcagcccc
      721 cggcctttac tccctctcat ttattcacat tacaaggatt tcatacatga aaacattgtc
      781 tcagaacacc acctcttcgg tctcagcacc ggagactgac ctgcaacaac tggttgccac
      841 catagtccct gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccaca
      901 gtgggtgacc ctggagcccc gtatcttcgg ctggatggac cgtctttgcg aagactactg
      961 cggtggtatc tggaatctgt acaccctgaa caacggtggc gcatttatgg cacccgaacc
     1021 ggatgacgat gatgacgaaa catgggtgct gttcaatgcc atgaacggta accgcgctga
     1081 aatgagcccg gaatccgccg gtattgccgc ctgtctgatg acgtacagtc atcatgcctg
     1141 tcgtacggag aattatgcca tgacggtcca ttattaccgg ctgcgggatt acgccctgca
     1201 gcatccggaa tgcagcgcca ttatgcgcat catcgactga atggaggcag ggacaatgca
     1261 gcagctttcc tttctgcccg gagagatgac gcccggcgag cgcagcctca ttcatcgggc
     1321 cctgaaaacc ctggaccgcc atcttcatga acccggcgtg gccttcacct ccacccgtgc
     1381 ggcacgggaa tggctgattc tgaacatggc aggactggag cgtgaagaat tccgggtgct
     1441 gtatctgaac aaccagaatc agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa
     1501 ccgcacggaa gtccatcccc gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc
     1561 cgtggtgctg gcacacaatc acccgtccgg tgaagtcaca cctggtaaga ctgaccggct
     1621 tatcactgaa cgtctggtac aggcgctggc cctggttgac atccgggtgc cggaccatct
     1681 gatagtcggt ggcagccagg ttttctcctt tgcggaacac ggtctgcttt aacccgtcac
     1741 cgtcaccacc acatcatacc tgttttcgct tttatcttct gtcttcagag gtatcacatc
     1801 atgagaatca tcacccgtgg tgaagccatg cgtattcacc aacagcatcc tgcatcccgt
     1861 ctttttccgt tctgtaccgg taagtatcgc tggcacggta gcgctgaggc gtataccggt
     1921 cgcgaagtac aggatattcc cggcgttctt gccgtgtttg cagaacgccg taaggacagt
     1981 tttggctcgt atgtccggct gatgagcgtc acactgaact gagtcaggag aaatgatgag
     2041 cagaattatc gctgccaccg gaccattctt aactgatttt ttgtgaggat tttatcgtgt
     2101 caggcacact ccatgagaca aattatccca acgacaacaa cgaccgcccc tggtgggggc
     2161 tcccctgcac cgtgacgccc tgtttcgggg cacgtctggt gcaggagggc aatcaattac
     2221 attaccttgc cgaccgcgcc ggtatcaggg ggcagtttag cgacgcggat gcgtatcatc
     2281 tggaccaggc ctttccgctg ctgatgaaac aactggaact catgctcacc agcggtgaac
     2341 tgaatccccg ccatcagcat accgtcacgc tgtatgccag agggctgacc tgcgaagccg
     2401 acaccctcgg cagctgtggt tacgtttata tggctgttta tccgacactg gcacccgcaa
     2461 ccacctcata accccgaata tctcaccttt agcactacca gttacagaga atttaagatg
     2521 aaaacattac ctgacacaca cgtacgggag gcatcgggct gcccgtctcc cgtcaccatc
     2581 tggcagacac tgctcacccg actgctggac cagcactatg gcctcacgct gaatgacaca
     2641 ccgttcgctg atgaacgtgt gattgagcag catattgagg cagggatttc actgtgtgat
     2701 gcggtgaact ttctcgttga aaaatacgcg ctggtacgaa ctgaccagcc gggattcagc
     2761 gcaggaaccc cgtcgcagtt aatcaacagc attgatattc tccgggcccg ccgggcaacc
     2821 ggcctgatga cccgcagcca ctacagaacg gtaaatgaca ttaccctggg taaacgcacg
     2881 gaggcaaaac gatgaacctt tccctgacgc tcgaagccga cagccttaac gtacaggcac
     2941 tgaacatggg acgcattgtc gttgacgtcg atggtattga gctcgctgaa ctgattaaca
     3001 atgtcactga aaacggttat tcagtccgcg tggtggagga ctccgggcaa cagtcaacct
     3061 gcacaccacc accgttaaca atccttgccg gcatacgctg cagcaccgca catatcacgg
     3121 aaacagacaa cgcctggctg tactcgctgt cgcatcagag cagtaacttc ggcgaatcag
     3181 aatggattca ttttacaggt agcggatatc tgttacgtac tgatgcgtgg tcatacccgg
     3241 ttctgcggct taaacgcctg ggcctgtcca gaacgttccg ccgtctggtt gtcacactta
     3301 cccgacgtta tggcgtcagt ctcattcatc tggatgccag cgccgaatgt ctgccggatt
     3361 tacccacttt cgactggtaa ccagaaacaa catgaaatca ttaaccacgg aaacagcgct
     3421 ggatattctg attgcatggc tgcaggacaa tatcgactgc gaatcgggaa ttatctttga
     3481 caacgatgaa gataaaacgg attcggcagc actgttgccc tgtattgaac aggccaggga
     3541 ggatatccgt accctgcgcc agctgcagct tccagaccag aaccggtgag tctcattcac
     3601 cactcttcat tccactgacg ccagcctgaa tacggctggc gttttcgttt atctgtaaaa
     3661 aggaatatcg attatgtctg atatcacagc ctcccgtccg gaagtggtca acgggcatac
     3721 ggacgttatt tgctcaacct cagtcagcca cattctggct gtacgaaaga gtacactgct
     3781 gcaaatcgac acacttatcc ggcaactggc tgaaatctca gttatgacgg aaagcattgg
     3841 cggtaaaact gcactggact gggccatgaa acaggatttt cgctgcggct gctggctgat
     3901 ggagaaaacg gaaaccgcaa tgaaagctat cacccgcaac cttgaccgcg aaatctggcg
     3961 tgacctgatg caacgttcag ggatgctgtc attaatggat gcacaggcac gtgatacatg
     4021 gtaccgatca ctggagtacg ataattttcc ggaaatcagc gaggcgaata tcctgagtac
     4081 gtttgaacaa ctgcaccaga ataaggacga agtatttgag cgaggtgtga tcaacctctt
     4141 cagagggctg agctggaatt acaaaaccaa ttgcccctgt aaatttggca gtaaaattat
     4201 cgtcaacaac ctagtgaggt gggaccggtg ggggtttcat cttatcaccg ggcaacagac
     4261 tgaccgactc gtcgacctgg aaagaatgct acatctgttc agcggcaaac cgatccccga
     4321 caaccgggaa aacatcacta ttcgtctgga tggtcacatc cagtccgttc agggtaaaga
     4381 gcgctatgaa gacgagatgt tcatcattaa atactttaag aagggatctg cacacatcac
     4441 tttcaaaagg ctggaactga ttgacagaat taacgatata atagccaggt actttccttc
     4501 tgtgctctca gcctgatccc gagtgtgatt ccctttcgat atcaaaaggg actgcgggta
     4561 taattgaggg tatattttta atcaaaccaa acaaattata ttaatatcaa tatgataaaa
     4621 gaaattttcg attccgagtc cggcaccaaa ttcataaggc ctcgctgaaa agcggggctt
     4681 tttgcttttc tggctacggt agatttctgg tcgttgcaat acgttatttg tttgttgtat
     4741 ttctcactat tttcgccgat gttcgttact tttagacacc ccaacatcct atgatgtgtg
     4801 ccttgtcctt actctactac tacggtcttt ctacggttcc cttttctcag aacattcata
     4861 atgaagtaga aaacacgggg aaacaggaga gttatcattt cgccttaatc cgcaggtttt
     4921 aacgtgattt tgtcgcggag aataaggtgg gaaaatcttc aataacaagc gggaaaacct
     4981 gatacgccat tgacatgggg ctcttcacca ctccgtccac ctgcaacaga ctcttctttc
     5041 cttctcgctt cttaccctta ccagaatcct ctgtcctcca cgagatcata aaatgatgta
     5101 tcagaagtaa tacgacttga acagtacaaa atagtggtac atttggaatc gaagtgtaaa
     5161 ggtaacttac tgttatttgg tgatttcatt gccgtgtcaa tgaatcctta attcgattcc
     5221 gaatcaggca ccagactcat atcaacggac ctccatggag gtccgttttt catccataat
     5281 cgccttcatt tgatgctgag ctgaacatat caacatcagg caacggcaca atctgtgtac
     5341 aaactccgcc agccctcaga taacacatca ctgagtaaat ggtcaggtat tgcttatatt
     5401 atgcccctct gcatctatta ttgctgccat ccactgagct tctgaggccg acaattttag
     5461 ccgtggagct cgctcccccg caccaggtgg cttcatatca aaaccataag agcttctgga
     5521 tataaccgtt ataccatcca cattacatgt tgttgatatc agttcacaat ttttagttga
     5581 tggcttatca attattggat cagatatttc tggtcgacgt cctgtgtgac atgaaaaaca
     5641 acttccgatt gacaatggca taattttatc tcctgccgat attactttac tgatatgcac
     5701 tgtcataata gaagtgcatt atttagagta ttcagatatc aatccagaac gtttcggatg
     5761 ataatctttc cattctggca tgcctaaaat tttctctgcc ggaacttctt tcccacgtag
     5821 ataaaacttt atatttttca ttatctgctc attatcctga ttacgataaa tccatcgcaa
     5881 ctcactggct gtaatacttt gcccataagc atccccctta ttggctatat tttttagagt
     5941 atcgacaagc ccatcggcac aaaaatgcac cattttgttc cttactaata actgaaactc
     6001 caggccagcc ttactggttt ttgcccataa cttgtcacga ttacacacag aaagatcggc
     6061 catagtgaca ttatattgac tatggttctt caaatactgc ttaaagttaa ttcctctatc
     6121 agactcactt aatgctctct tgataagagc gtctttctta tatttccgag gagatttata
     6181 atcccataca gcacttgtta acgtattctg tattaaaata tcttttttag aaaaatcatt
     6241 aaccagtttt attgtattaa caacttttcc tctgggttcc gaaagcccat acacaatatc
     6301 ccctagacga aaatgtaaag ccgcctccat ggcatagccc tgaaaagtaa tatttttttc
     6361 tttaatcaca tctggagaaa gctgtacatt ctgtaaatca agatccatct cttcgggaaa
     6421 tccttcagca acgatgttcc atgtcgtttt ctgctctgta taaatggttg ctgacaccat
     6481 cccttttggg agtttttccg gcaacacgat attactgcaa cccgtcagag acaacgtctg
     6541 cagtgaaggg gatataaaat tatcgattct gacctgatac tccgggttat aatcccggat
     6601 actcagagaa cacaaccctg atggtacgcc ggtaagcgtc tccgcagccc tacccgtcaa
     6661 ctcaagagac cggacactat caggcacacc cgatacctga cagtgaccaa gtttcaaatg
     6721 ctcaacgccc tctggaacag tatgtacaaa agacgaatta ttcaattcaa tatgtagctc
     6781 tcttactgtc ggcggcagtt cactgacttt cagattctca caattaataa cggacaggct
     6841 ttttatattg accggtagcc ctgtaagacc tgtcagctta ttacagttct ctattgttat
     6901 cgctgtgatt tcttccggta aatgaggcaa gaactccatg tcacaatttt ttatatgcag
     6961 acaaccactt tcccgtgcat cttccagttg ccgggacgta atttcacttt taaagcttgc
     7021 aggtgtttca gtaatatgat gacaggcagt ccctgacaat tttaattgtg atgtggaaag
     7081 catcaataaa ttccattttc agaaaataaa tcactataag gtaagcccca tccgaggagc
     7141 ttacctgaca aatttcatac agagagcctg tatccagggt ttcccgatgg cgaaaaggca
     7201 tcattacaga cgctatttca cgctggaaaa gcaagtacag ttactacatg ttcattccag
     7261 aataaaccca gcaatctgac ccggattcta acaaccagca aaataatgtt gtcaatcgaa
     7321 gacgaataaa aagcagcagt cagtacaact cagtctgtat taatatcccc cactcaaaaa
     7381 cgtacatcct gcccggaaat gatgcagaaa cgctgatcag ttagatcaaa ataacactcc
     7441 tctctgccaa cgatcatttc tggagaaaac gcttcacgac tgaggggatg aaaaacattg
     7501 tgtcggatag cctcagacaa ggcatcctta tcgtataaag aacagacttt tgaatgcagt
     7561 gcatttttaa caaaaacgcc tgtttttggt atacataatg ttatcgggca actcagatgt
     7621 tgctcacagc aaggaaaatc tccaggattg acggtaaatg catgagaatt aattctggtc
     7681 tgcactgcat ttctgccccc ttccaaccag aatggggtta aacgaacctg ctccatatat
     7741 aaactgaacg cctgcaggaa agtattgcca tgattaagct gtctctccag actctctgca
     7801 aggcgacgac acctccctcc aagacggtca agaagactgc cctgaatcgg ctccacacta
     7861 aaagcatcca taaatgaaat atggtgcagc ctcagactac gacttcccac aatcagagta
     7921 tcactctgat tgcttcttgc tatattacgc agagcttcca gttcactttc aggcagcgcc
     7981 cttccatctg agatatttaa tacgacgggc atattatctc cctctgtaat gtatttagct
     8041 tatccaggcc aacaacaaat aaacatccac acataaaata aaacagacaa aacattttgg
     8101 caacatacca tcaattattg acttatcatt atttacacca aagcgttgcg atactaaaat
     8161 aattataaac ttgctacatt gtaaatatag acaaataaaa ggaattacta tgccagcaaa
     8221 tatatcagga acacccttca actcatttgg tataagcttt atccagaaac agtcctgctg
     8281 gaggaaatct gatgatatcc tgcgatgttc aatgggtcag aggactatca agttatccac
     8341 taatacgctg aacaaccgaa ttttgacctc cgtcgcccga caaagcacca aagatattaa
     8401 tgcctggaaa agagatgaac agacagttta cccatctcgc gtgatcaacc agggaattga
     8461 taaatattgt gctgaaaata gcagaaacat ttccagtgaa gtacggcaaa gagtttttaa
     8521 attcattgaa aaggattaca gtctcaagtt gaatatcata gcagcgcaaa gctctatcaa
     8581 ccatctcata ataggaaatg gccgtttcgg tgataaaata aatatgcttt gcaagggagt
     8641 gagtagagaa gtaaagaacc agacaatgga tgtaatagcc aatcaactcg cagaccaatt
     8701 cttccaaaag catattagtc cagatgttga catcaaacag ctacgagatg acatccccag
     8761 atatattatg gctgccagcg tcatatcggc ataaatagag atacccttaa cgctaaccag
     8821 aaaaaaaata cctataagag cctgtacata gagttgtgtc gttgcctgat gttgatatgt
     8881 caaatccaac atcaaatagc ggtaaattta tggataaaaa attacaggct ctggttaacg
     8941 aaaccccccc ctcttgctct atccccctct ttcatcattt attacaatat ttatttcaat
     9001 aaaaaccgat attaccaatc acatttatca caatgcaact taatatcggt ttttatcttt
     9061 taatgcctga gcaataggcg gtaatggccg ggatgcctcg gatgcctgtc cacttgtcgg
     9121 tggcggcggt gccgggcggc ttggcttaag acctgaagta tcaagacttt tcgatttttc
     9181 ataggcagcc aaatgatctt ttagcgcctg agcaatgggc ggtaaagacc gggatgaccc
     9241 agataccaac ccgcttgtcg gcggcggcgg tgccgggcgg cttggcttaa ggcttgaagt
     9301 atcaagactt ttcgactttt cataggcagc taaatgatct tttaatgcct gagcaatggg
     9361 cggtaaagac cgggatgatc cagatacctg cccacttgtc ggcggcggtg gagccgggcg
     9421 ggccggctta aggcctgaag cctcaaggct tttcgatttt tcataggcag ccaaatgatc
     9481 ttttaatgcc tgcgcaatgg gcggtaaagg gcgggatgcc ccggaagcct gtccacttgt
     9541 cggcggcggt ggggccggac gagagggagt aaatgaagtc gccgggctac tcacattaaa
     9601 agtcgttctc gcattgatat tcgacgtacc gggagaaaaa ggagattgag ccctaatcgg
     9661 attcagacga acagcctgag gggacactga aaagccagat aaccccgcag agcttacccg
     9721 gcttgccgca ctagtaatat gccgtcctag tgtagaaaca gcttgactaa ttccattaag
     9781 cataattcat gcctttttat atctaatcaa caggaataat tattaattta tattaaaatt
     9841 aaaaatagtt aacgactata aactattaaa tcaaacacac atg
//
LOCUS       DAZGCS010000152         9583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGCS010000152 DAZGCS010000000
VERSION     DAZGCS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.154"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..888
                     /gene="xapR"
                     /locus_tag="ACHK01_004428"
     CDS             4..888
                     /gene="xapR"
                     /locus_tag="ACHK01_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO8099259.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLRIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(997..1072)
                     /locus_tag="ACHK01_004429"
     tRNA            complement(997..1072)
                     /locus_tag="ACHK01_004429"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1037..1039),aa:Lys,seq:ttt)
     gene            complement(1077..1152)
                     /locus_tag="ACHK01_004430"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHK01_004430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Val,seq:tac)
     gene            complement(1199..1274)
                     /locus_tag="ACHK01_004431"
     tRNA            complement(1199..1274)
                     /locus_tag="ACHK01_004431"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Val,seq:tac)
     gene            complement(1319..1394)
                     /locus_tag="ACHK01_004432"
     tRNA            complement(1319..1394)
                     /locus_tag="ACHK01_004432"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1359..1361),aa:Val,seq:tac)
     gene            1653..3068
                     /gene="gltX"
                     /locus_tag="ACHK01_004433"
     CDS             1653..3068
                     /gene="gltX"
                     /locus_tag="ACHK01_004433"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO8099260.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(3120..3512)
                     /gene="yfeD"
                     /locus_tag="ACHK01_004434"
     CDS             complement(3120..3512)
                     /gene="yfeD"
                     /locus_tag="ACHK01_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8099261.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3514..3873)
                     /gene="yfeC"
                     /locus_tag="ACHK01_004435"
     CDS             complement(3514..3873)
                     /gene="yfeC"
                     /locus_tag="ACHK01_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8099262.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4094..4169
                     /locus_tag="ACHK01_004436"
     tRNA            4094..4169
                     /locus_tag="ACHK01_004436"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4127..4129,aa:Ala,seq:ggc)
     gene            4209..4284
                     /locus_tag="ACHK01_004437"
     tRNA            4209..4284
                     /locus_tag="ACHK01_004437"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4242..4244,aa:Ala,seq:ggc)
     gene            4492..6681
                     /gene="pdeA"
                     /locus_tag="ACHK01_004438"
     CDS             4492..6681
                     /gene="pdeA"
                     /locus_tag="ACHK01_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8099263.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6731..7933)
                     /gene="nupC"
                     /locus_tag="ACHK01_004439"
     CDS             complement(6731..7933)
                     /gene="nupC"
                     /locus_tag="ACHK01_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO8099264.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8269..9507
                     /gene="mntH"
                     /locus_tag="ACHK01_004440"
     CDS             8269..9507
                     /gene="mntH"
                     /locus_tag="ACHK01_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO8099265.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
BASE COUNT         2303 a         2248 c         2507 g         2525 t
ORIGIN      
        1 attatggaac gcgtatacag aacagatctt aagttgctcc gttattttct tgccgtagcg
       61 gaagagttgc attttggccg cgcagcagcg cgtttaaata tgtctcagcc tccgctcagg
      121 attcatatta aagagctgga aaatcaactc ggcacgcagc tttttattcg ccattcgcgc
      181 agcgtcgtac tgacacacgc gggcaaaatc ttgatggaag aatcgcgtcg attgctggtg
      241 aatgctaata atgtattggc tcgggttgaa caaataggtc ggggagaagc agggcggatt
      301 gaactcggcg tagtgggaac ggctatgtgg ggacggatgc gcccggttat gcggcgattc
      361 ctcagggaaa atcctaacgt tgaagttctt tttcgcgaaa agatgcccgc gatgcaaatg
      421 gccctgctgg aacgacgtga actcgatgcc gggatctggc gaatggcgac agaaccaccg
      481 acaggtttta ccagcttacg gttgcatgaa tcggcgtttc tggtggcgat gcctgaagag
      541 catcatctct catcattttc caccgttccg ctggaagcgc tacgtgacga gtattttgtt
      601 acaatgccgc ccgtgtacac tgactgggat tttttgcagc gagtttgcca gcaggtggga
      661 ttttcaccgg ttgttattcg cgaagttaat gaaccgcaaa cggtactcgc catggtcagt
      721 atgggtattg gtatcacatt gatagcggac agctacgcac aaatgaactg gccaggtgtc
      781 attttccgtc cgctcaaaca gcgcatccct gcagatttat atattgttta tgaaacacag
      841 caggtgacgc ccgcgatggt taagctgttg gctgcattga cacagtaggt gaggagacag
      901 gaattgccgg aaatagtcgt ctgctaagtg aagcgcatca ggcaatttcg caaatcgcag
      961 atagcaaaaa agcgccttta gggcgctttt ttacattggt gggtcgtgca ggattcgaac
     1021 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgaagtggt
     1081 gggtgatgac gggatcgaac cgccgacccc ctccttgtaa gggaggtgct ctcccagctg
     1141 agctaatcac ccacttcggt acttcacatt tttacaagaa atttagctgg cgagaaagtg
     1201 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
     1261 tgagctaatc acccacttct caatttcttg ctacacggcg gagactacat aaagtagttg
     1321 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
     1381 tgagctaatc acccccgctg tgtggagtcg cattataggg agagttcaaa atgagtcaac
     1441 gcattttcta aagaaattgt tcgttcgtcg taaatttaag caagatgatc gcaaaacaga
     1501 ccgtgttgcg caatttgtca acgaaaacaa taatgcgtaa ggtagaaacc cgaactacat
     1561 tgaggaatca ggcgggagtg atagaatatc gcccactcaa tttttccagg atttgccggt
     1621 tgtcggcatc tttctaaacg taaggccatt tcatgaaaat caaaactcgc ttcgcgccaa
     1681 gcccaacagg ctatctgcac gttggcggcg cgcgtactgc tctttactcc tggctttttg
     1741 cacgtaacca cggcggtgag ttcgtgctgc gtattgaaga caccgatctt gagcgttcca
     1801 cgccggaagc tatcgaagcc attatggatg gcatgaactg gctgagcctg gagtgggatg
     1861 aaggtccgta ctaccagacc aaacgttttg atcgctacaa tgcggtgatc gatcagatgc
     1921 tggaagaggg cactgcttat aaatgctatt gctctaaaga gcgcctggaa gcgctgcgcg
     1981 aagagcaaat ggcgaaaggt gagaagccgc gttatgacgg tcgctgccgc cacagccatg
     2041 agcatcatgc tgatgatgaa ccgtgtgtcg tgcgttttgc taatccgcag gaaggttctg
     2101 ttgtttttga cgatcagatc cgtggtccga tcgagttcag caaccaggag ctggatgatc
     2161 tgattatccg ccgtaccgat ggttccccaa cctataactt ctgtgtggtt gtcgatgact
     2221 gggatatgga aatcacccac gttatccgtg gcgaagacca tatcaacaac acgccgcgcc
     2281 agatcaacat tcttaaggcc ctgaaagcgc cggtgccggt ttacgcgcac gtttctatga
     2341 tcaatggcga tgacggtaaa aaactgtcca aacgtcacgg ggcagtcagc gtaatgcagt
     2401 atcgtgatga cggttatttg ccagaagcac tgctgaacta tctggtgcgt ctgggctggt
     2461 cccacggcga tcaggaaatc ttcactcgtg aagagatgat caaatacttc actttgaatg
     2521 ccgtcagcaa atctgccagt gcgttcaaca ccgacaagct gctgtggctg aaccatcact
     2581 acattaacgc gctgccgccg gagtatgttg ctactcactt acagtggcac attgagcagg
     2641 aaaatatcga tacccgtaac ggcccgcagc tggctgatct ggtgaaactg ctgggcgaac
     2701 gctgcaagac gctgaaagag atggcacaga gctgccgtta tttctacgaa gattttgctg
     2761 agttcgatgc cgatgcggcg aaaaaacatc tgcgtccggt agcgcgtcag ccgctggaag
     2821 tggttcgtga caaactggcc gcgattactg actggaccgc tgaaaacgtt catcacgcta
     2881 ttcaggcgac ggcggatgag ctggaagtgg gtatgggtaa agttggtatg ccgctgcgtg
     2941 tcgccgtaac cggtgcgggg cagtctccag cactggatgt taccgttcac gcaattggta
     3001 agacccgcag tatcgagcgt atcaacaaag cgctggattt tattgctgaa cgcgaaaatc
     3061 agcagtaatt agcgtgtaaa aagataaacg gcaggagata atatcctgcc gttttttatt
     3121 tatgctgatt cgtcaatatc gagacgggcg aggaaattgc gaattccttc acgtgacaaa
     3181 aattgcgcga ctttttcctg ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt
     3241 cgatacgccg gggcgcgtgc tccaggagcg tattcagcaa ttctctcttc agcttccatc
     3301 aacattggtg tgttatggaa ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg
     3361 accagaataa gtctggcacg accgcctttc acgccgggaa atttttccgt tttccagcct
     3421 ttttctctga tccaacggtt aacagtttgt ttagccacac cgaggcattc agccagttct
     3481 tcggtggtca ttttgttgcg taatcttttc atattatttg ctatcgcgaa tccctaagcg
     3541 ttgtaacaat ccgataatcc cttcccgcag aagcaaggat gtaaactgtt tttgttcaac
     3601 tggcgtcatc tctttcgcca gtgtcaccag caatacttca agcggcgcat caccagaaag
     3661 ggcaggcgct tctccctgac cttctggacg ttcagcattg cgaatatatt cacgaacctg
     3721 ttcattgacg tgaaccagtc gggctttgcc accctggacg ccaggttttg gtgacgttgt
     3781 ccagccttcc ttgcgtaccc atttattaat ggtctggcgg ctatagccgg tcagtctggc
     3841 aagttcatct ggtgtcatcc tctccttgaa cataacaatt ccctgaatgt atgtgggtct
     3901 attagtagtt cattttataa caccacttta caggaaaccg tgacatgaat aactactgaa
     3961 tgcgagcggt tttccaaaat tgttcatttg cctgattttt cagcgattga aattacacgt
     4021 tgcaatttgc cgttgacaca ttcgggcgga atttatatga tgccgcccgt caactcgaca
     4081 agctttacgt gacggggcta tagctcagct gggagagcgc ttgcatggca tgcaagaggt
     4141 cagcggttcg atcccgctta gctccaccaa attttgcacc cagcaaattt ggtacgtaaa
     4201 cgcatcgtgg ggctatagct cagctgggag agcgcttgca tggcatgcaa gaggtcagcg
     4261 gttcgatccc gcttagctcc accaaatttc caaccctcgc tgcaaagcgg gggttttttg
     4321 tctctgcttt ttgccgcttt tgtcatacag tctacgtccg ggttagcgcc gcctggtgaa
     4381 agcatcattg gatgaaaaat cggcaacagg ctggcccctg tttgcttcgc gatgcgaata
     4441 aacttattat ttgtgtgcct gaaaaccccg atcagtgaga gtagtgtact catgtttgtg
     4501 gagcataacc tgataaaaaa tatcaagata ttcacactag cgtttacgct caccgtggta
     4561 cttattcagc tatcccgttt tatttcgcca cttgccatta tccattccag ttatatcttt
     4621 ctggcgtgga tgccactgtg cgtaatgctg tcaatcttgt ttatctttgg ctggcgcggt
     4681 gtcgttcccg ttttatgcgg gatgttttgc accaatctgt ggaactttca tctctctttt
     4741 ttacagactg cggtcatgct cggtagccag acgtttgtcg tgttgtgtgc ctgcgcaata
     4801 ttacgctggc agctggggac gcgttggcgt tatggattga ccagccgata tgtctggcaa
     4861 cgtctgttct ggcttggttt ggtgacgccg atcggcatca aatgcagcat gtatcttgtg
     4921 gggagcttct ttgatttccc gctaaagata tccacctttt tcggcgatgc ggatgccatt
     4981 ttcacggtcg ttgatttgct aagccttttc accgctgtgc tgatttacaa catgcttttc
     5041 tactatctca ctcgcatgat tgtaagtccc cactttgcgc agatattgtg gcgcagggat
     5101 atcgcgccgt cgttgggcaa agagaaacgc gcatttacct taagctggct ggcagcgtta
     5161 agcgtgcttc tacttctgtt gtgcacacct tatgaaaacg actttattgc cggttacctg
     5221 gtacccgttt tcttcatcat ctttaccctc ggggtcggta agcttcgcta tccgttttta
     5281 aatctcacct gggctgtttc aacgctttgc cttctgaatt acaaccagaa ctttttgcaa
     5341 ggggtggaaa ccgaatattc gctggcattt attcttgcgg tgctgatttc ctttagcgtt
     5401 tgcctgctct atatggtgcg catttatcat cgcagtgaat ggcttaatcg ccgctggcat
     5461 ttgcaggcgc tgacagatcc gttaacgctc ctacccaact ttcgtgcgtt ggaacaagcg
     5521 ccggaacaag aggcgggcaa gagtttttgc tgcctgcgca ttgataatct tgagtttatg
     5581 agtcgtcatt acggcttaat gatgcgcgtt cactgtatcc gctcaatttg ccgtacgctg
     5641 ctgccgttga tgcaggaaaa cgaaaagttg tatcaattgc cgggtagtga actgctgtta
     5701 gtgctgagcg ggccggaaac ggaagggcga ctccagcata tgattaacat cctgaatagt
     5761 cggcaaattc actggaacaa taccgggctg gatatgggct atggtgctgc ctgggggcgt
     5821 tttgatggaa atcaggaaac cctgcaaccc ttgttggggc agttaagctg gctggcggag
     5881 caatcctgcg cacatcatca tgtgctggcg ctggatagca gagaggagat ggtttccggg
     5941 cagaccacta aacaggtgct attgctgaat accattcgca cggcgttaga tcagggtgat
     6001 ttgctgctct acgcccagcc aattcgcaac aaagagggtg aaggttatga tgagatcctc
     6061 gcgcgactga aatatgacgg cggcattatg accccggata agtttctgcc ccttattgct
     6121 cagtttaacc ttagcgcgcg ttttgatttg caagtgctgg aatccttgtt gaagtggctg
     6181 gcaacacacc cttgcgacaa aaaaggaccg cgcttttcag tcaatttaat gccgctcacg
     6241 ctgctgcaaa agaatattgc cgggcggatt attcgtctgt ttaagcgtta tcacatctcc
     6301 ccgcaggcgg tcattcttga gatcaccgag gagcaggcgt tttctaacgc agaaagcagc
     6361 atgtacaaca tcgagcagct gcataagttt ggtttccgga ttgcgattga tgactttggc
     6421 accggatatg ccaactacga acggttaaag cgtttgcagg ctgatatcat caaaattgat
     6481 ggcgtctttg tgaaagatat tgtcacgaac acgctggatg cgatgattgt gagatcaatt
     6541 accgatctgg cgaaagcgaa gtcattgagt gtggtcgcgg agtttgtcga gacgcaacag
     6601 cagcaggcgc tattgcataa gctcggggtg caatatctgc aagggtattt gattggtcgc
     6661 ccgcagccat tagctgatta acaggcgtaa aaaaaccggg gaattatccc cggctttttt
     6721 atgtatggtc ttacagcacc agtgctgcga ttgacgcaga cagcacactc accagggtag
     6781 agccgtaaac cagcttcaga ccgaagcgag aaaccacgtt accttgctct tcattcaggc
     6841 ctttaactgc acctgcgata atcccgattg aagagaagtt agcgaaggaa accaggaaca
     6901 cagagatgat gccttcagca cgcggagaga gcgtggaagc aattttctgc agatccatca
     6961 tcgcaacgaa ctcgttggaa accagtttgg tcgccatgat actgcccact tgcagtgctt
     7021 cactggaagg aacacccatc acccatgcaa tcggatagaa gatgtagccc aggatgccct
     7081 ggaaggagat gctgtagcca aaccagccag taacggtggc aaacagtgcg ttcagcgcgg
     7141 cgatcagggc gataaagccg atcagcatcg cggcaacgat aatggcaact ttgaaacctg
     7201 ccagaatgta ttcacccagc atttcgaaga agctctgacc ttcgtgcagg ttggacatct
     7261 ggatgttttc ttcactggca tcaacacggt aaggattgat cagcgacagc acgataaagg
     7321 tgctgaacat gttcagtacc agcgcagcaa cgacgtattt cggttccagc atggtcatgt
     7381 atgcaccaac gatggacatc gacacggtgg acattgccgt ggcagccatg gtgtacatac
     7441 gattacggga gattttgccg aggatatctt tataggcaat aaagttttca gactgaccca
     7501 gaatcaggga gctgacggcg ttaaaggatt ccagtttgcc cataccgttg actttggaga
     7561 gcaggaaacc aattgcgcgg ataataaccg gcaacacgcg aatgtgctgg agaataccga
     7621 tcagtgcaga gataaagacg attgggcaca gcactttcag gaagaagaat gccaggcctt
     7681 gatcattcat gctaccaaag acgaagttag tcccttcgtt ggcaaatccg agcagttttt
     7741 cgaacatttc ggagaagcct ttcacgaagc ctaaaccaac gtcggagttc aggaagaacc
     7801 acgccagtaa cacttcgata acaagcagtt gaataacata acggatacga atttttttgc
     7861 ggtcgctgct taccagcagt gcgagaatcg caacaacggc aagtgccagt acaaaatgaa
     7921 ggacgcggtc catatttgct ccaaatatga ggcaggttaa atttccgtgc acattctatg
     7981 taacatggtt aaggaaaacg agatctgaca cacactataa tgacgtcctg tgacaagctt
     8041 caaaaatagt gatctgtcat acatttctgc tatgttgtgt atggaagctg aaagttatgt
     8101 aaatgtgcta acattaaaca taaccataac attcaaatca tcaatctttt cgtagtcata
     8161 atcctggtct atcaaagaaa tcaccacaac ccattcaaat gaatttgata atcattctcg
     8221 tttggcatag catgaaacat agcaaaggct atgttttaga ggcacaagat gacgaactat
     8281 cgcgttgaga gtagcagcgg acgggcggcg cgcaagatga ggctcgcatt aatgggacct
     8341 gcgttcattg cggcgattgg ttatatcgat cccggtaact ttgcgaccaa tattcaggcg
     8401 ggtgctagct tcggctatca gctactgtgg gttgtcgttt gggccaacct gatggcgatg
     8461 ctgattcaga tcctctctgc caaactaggg attgccaccg gtaaaaatct ggcggagcag
     8521 attcgtgatc actatccgcg tcccgtagtg tggttctatt gggttcaggc agaaattatt
     8581 gcgatggcaa ccgacctggc ggaatttatt ggtgcggcga tcggttttaa actcattctt
     8641 ggtgtttcgt tgttgcaggg cgcggtgctg acggggatcg cgactttcct gattttaatg
     8701 ctgcaacgtc gcgggcaaaa accgctggag aaagtgattg gcgggttact gttgtttgtt
     8761 gccgcggctt acattgtcga gttgattttc tcccagccta acctggcgca gttgggtaaa
     8821 ggaatggtga tcccgagttt acctacttcg gaggcggtct tcctggcagc aggcgtgtta
     8881 ggggcgacga ttatgccgca tgtgatttat ttgcactctt cgctcactca gcatttacat
     8941 ggcggttcgc gtcaacaacg ttattccgcc accaaatggg atgtggctat cgccatgacg
     9001 attgccggtt ttgtcaatct ggcgatgatg gctacagctg cggcggcgtt ccacttttct
     9061 ggtcatactg gtgttgccga tcttgatgag gcttatctga cgctgcaacc gctgttaagc
     9121 catgctgcgg caacggtctt tggattaagt ctggttgctg ccggactgtc ctcaacggtg
     9181 gtggggacac tggcggggca ggtggtgatg cagggattca ttcgcttcca tatcccgctg
     9241 tgggtgcgtc gtacagtcac catgttgccg tcatttattg tcattctgat gggattagat
     9301 ccgacacgga ttctggttat gagtcaggtg ctgttaagtt ttggtatcgc cctggcgctg
     9361 gttccactgc tgattttcac cagtgacagc aagttgatgg gcgatctggt gaacagcaaa
     9421 cgcgtaaaac agacaggctg ggtgattgta gtgctggtcg tggcgctgaa tatctggttg
     9481 ttggtgggga cggcgctggg attgtagttg aatgagcgtc gcatctggca ctattggcgg
     9541 atgcggcgta aacgccttat ccgccctacg cgttaaagag ccg
//
LOCUS       DAZGCS010000153         9413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGCS010000153 DAZGCS010000000
VERSION     DAZGCS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.155"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(618..1148)
                     /gene="yodB"
                     /locus_tag="ACHK01_004441"
     CDS             complement(618..1148)
                     /gene="yodB"
                     /locus_tag="ACHK01_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8099266.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1491..2141)
                     /gene="zinT"
                     /locus_tag="ACHK01_004442"
     CDS             complement(1491..2141)
                     /gene="zinT"
                     /locus_tag="ACHK01_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO8099267.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2398..3033)
                     /gene="msrQ"
                     /locus_tag="ACHK01_004443"
     CDS             complement(2398..3033)
                     /gene="msrQ"
                     /locus_tag="ACHK01_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO8099268.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3034..4038)
                     /gene="msrP"
                     /locus_tag="ACHK01_004444"
     CDS             complement(3034..4038)
                     /gene="msrP"
                     /locus_tag="ACHK01_004444"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO8099269.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4147..4560)
                     /gene="hiuH"
                     /locus_tag="ACHK01_004445"
     CDS             complement(4147..4560)
                     /gene="hiuH"
                     /locus_tag="ACHK01_004445"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO8099270.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4693..5364
                     /gene="hprR"
                     /locus_tag="ACHK01_004446"
     CDS             4693..5364
                     /gene="hprR"
                     /locus_tag="ACHK01_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO8099271.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5364..6722
                     /gene="hprS"
                     /locus_tag="ACHK01_004447"
     CDS             5364..6722
                     /gene="hprS"
                     /locus_tag="ACHK01_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO8099272.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEVDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6829..7680)
                     /gene="hchA"
                     /locus_tag="ACHK01_004448"
     CDS             complement(6829..7680)
                     /gene="hchA"
                     /locus_tag="ACHK01_004448"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO8099273.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8273..9346)
                     /locus_tag="ACHK01_004449"
     CDS             complement(8273..9346)
                     /locus_tag="ACHK01_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8099274.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2618 a         1957 c         2151 g         2687 t
ORIGIN      
        1 ggagtagggg caactctacc gggggttcaa atccccctct ctccgccact ttatcaatga
       61 cttatctccc gacttcccgc cttgcttttc ctaaacagaa caatcgtaga atattcttga
      121 agggttagat cgtcactgtt ttctgttcga tactgtgaca ttcagcactt gattcgctat
      181 ggatctgaca ggaagctttc gagcgaaaat gtgcagttat tcagtcgttt tcttatcgct
      241 caccattatt cttttagaca ttcatcctat gaaactgccg caaagttggt ggtgggaact
      301 gaagttgcgt agagaagggg tcaataattg agtcaaaaaa gtaacgcggc cgtcagatta
      361 aagccgcggg acaaagtcca taatttccaa gagttgttga atttcccagc ttttttgaaa
      421 gcctttgtat catactaaca agtaaaaaag gtttaaatac caaaattaac ctgtattttt
      481 gataagtctt accattgtac gatttacaca gtttctgctc ctccttgttg atagtggaaa
      541 gggtgcattg tgcacttgcg taaacattta tgacgcgttg atgtccatgg ttcacatttc
      601 cttgtcaggg gatcgactta tttgcggcga ggcatcattc gtaacagagt attatctttc
      661 tgaatgtagt gatgaaagag tgctgcgcca gcgtgagctg cgattaaaaa gtaacctata
      721 tttgcccagg tttcgtgaat attttttatc agtgctttaa tttcgctgtt tggggtaaca
      781 aagggagaca cattgaaacc aaggaaactc cacgattttc cactgtaagc catcaaagca
      841 atccccagca gaggaagtgc aaggaaggtt atataaagca tgatgtgcat tagcgaagcc
      901 gctttcattt gccaggcagg tggcggtggc acaatagaag gatcactata acggtgtttt
      961 ataatcaggc gtgaaaacat taacacccaa acgaatatac ccgcattgta atgtgtttct
     1021 cgcatcagca gataagtact actaccttta ggaaaccagc cacggagttc catcgcggca
     1081 taggttattg caacgaaaag cagcgttatc caatgtaaat aaatttgagt ttttgagaat
     1141 cggttcattt ttgtagctcc tgtaattttt acagtcaagc tatcccacga accttaaaaa
     1201 atccttatac agatgtggtt ttaagaattt gttatttaaa aaattgatag tgcacattgt
     1261 aaccagccaa tataagcaat ggcgtgagca caggtatctt tctctgttgg ccgtattgtg
     1321 tatggaatcc gttattggtt aactcaatgc gggttagctt attaatatac agacacgtca
     1381 tagtgatgag cgtgtctgta gctagcttgc gttcagtggc gtttgtggat ggaccagtgg
     1441 cagttatgat gacttactaa agcggcatcg aggcgttatc atgagaatac tcaatgagac
     1501 atcatttcct cgaccacttc ttcgctactc aactgatatg gataatacgt tggccagttt
     1561 tccatttcat ttaataaaga ttgctgtgaa tcattaccca taaaaatgtg aaaatgagat
     1621 gattttcgtg gtgcaattat atgatcgcta aattgtatat atttaggggc tttgctttca
     1681 ggatctttac attcgaataa gtaacgaacg cctttcttgc ctgatttata ggtgagtatt
     1741 ttgtatccat cgtaatcata tttacaggat gttgtttcat tatttctatg gaattcaaca
     1801 atgccgtcct caatgccaat catctcgata tctgttgcat aacctttgtg ataataatct
     1861 ttaatttcag caaatgtttt agttttatct gcatccgctt tcttctgaaa gacggggtca
     1921 agtttgccac tttgcagtaa aggataaacg gattgccaga ctccatccca gtcactgagc
     1981 gttcggtttt gtacattggc atcatcaaaa acaccattag cagctttttg ttcgacctct
     2041 gttaagggtt tgccgtgtga gtgatgacca tgcgaaaagg caggagcgct aacaataaag
     2101 acacctaaag caacagccag tttgtgaaga cgaatcgcca aaacagtttc ctccagagtc
     2161 attgtatatg atgtgtaatg ttatattgta acatatggca aaatgcaaca actcttagga
     2221 gatgagagca tagttttctc aggtgtaatt atctatctta ttgaaataaa atgaattata
     2281 ttgataattt gaggttgatg acaaacgaaa cataacctga tgtgatctgc gtaggccgga
     2341 tagggcgctc gcgccgcatc cggcagggat aacgcgtact ttgtcggcaa tatgaagtta
     2401 tacagataat ttgttgtgaa cctgcttgcg taaccggtta aaaagggaac gcaacttctt
     2461 ataccgtaag gctaaaagca gtacagccag cccagcgtag atgagcggtt gcggtgaaat
     2521 aatcttcact gaccacagat aatgtattgg ggccaggatc gcgacaagat agacgaaatt
     2581 atgcaactgt tgccagtgct tgccgagttt tcgctgcatc gcctgggttg aagtaaacgc
     2641 taaagcaagc aaaataaccc agctgataat tcctaacgtt aaataaggcc gggtaattaa
     2701 ctccttgcct aataacgcca gattattcac gcccagctcc agcaatgcgt aactggttaa
     2761 atgcagtgtc gcccaggcaa agcaccataa gcctaacagg cggcgagtgc gtatcaataa
     2821 cggctgtttt gcgtagcgtg ccagaggtgt gattaacaag gtcgccagca aaaatttcag
     2881 tgcagtgcga ccggtaaaat gctgaatatc tttcaccgga tcggcaccca gtccaccgtg
     2941 attgatcgcc cagaccagcc agagaaacgg caacaatccg gcaagatgca ggctaacttt
     3001 cagccatgtc acctgttttg ctgtcagacg cattcagaaa ttctcccgca aatccaggcc
     3061 acgatacagc gatgccacct ggtcggcgta accattaaac agtagcgttg gctggcgctg
     3121 aacatcgagg atgccgcctg aaccaataaa tcgttcggta gcctgtgacc agcgcgggtg
     3181 atcaacatgc ggattaacgt tggcgtaaaa accgtattcg tcaggcgctg ccagattcca
     3241 ggtggttggc ggacgctcgc gggtcagctt aatactgact atcgatttaa tccctttaaa
     3301 gccatatttc cacggtacaa tcagtcgtac cggcgcgcca ttttgtggcg gtaacgcctt
     3361 gccataaaca cccacggtca tcagtgtgag cggatgcatt gcttcgtcga gacgcaatcc
     3421 ttcgacataa ggatatttca gcccgccgcc gataaagcgg tcctgctggc ctggcatttg
     3481 ttccggtgca taaattgttt cgaaagcgac atacttcgca ttgctggtgg gttcggcaag
     3541 cgccagcaat ttgtgcagcg gaaaaccaat ccacggcacc accatcgacc atgcctccac
     3601 gcagcgcata cgataaatac gctcttccag cgggaagcga cgggttaaat catcgtgatc
     3661 gagggtcaat ggttttgcca cttcgccgct gattttcagt gtccatggat cggttttcag
     3721 gctaccagca ttagcggcgg gatcggcttt atccagcccg aattcataga agttgttata
     3781 accggagact ttatctactg gcgtcagtgg caggttattt tgccaggcgg caggcttgct
     3841 gaactccagc ggttttccgg cgggggcggg cgggcgatcg ttccctttaa accagctaag
     3901 cagatcggca tgcgcagcgt gaggcaaaga aagtgcagct gcgctgatgc ccagtgcttt
     3961 taacacctgt cgacgcttca taaagaatac cgactcggcc gtaacatctg attcttttaa
     4021 aaattgattc tttttcataa gccatcacac ttttttgtcg ttagccagca agagccgttt
     4081 actgttaatc tgtcagggag tgccctccct aacagtagca gagatctgct tttacatatt
     4141 tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata aaggcacatg
     4201 ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga agaaactttc
     4261 aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat cgccagtagt
     4321 cgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt tggcggtatt
     4381 aagttgtaac cagccgttgt ccgctttctt ttcaagagtg actgtcacgt cggcagcggg
     4441 ttttccggtt tgctggttca aaatgtgcac gctaagaatg ttttgttgtg cggcataaac
     4501 caaagaaggt aatgaaaatg ctgccgttac tacggagagt actaaataac gctttaacat
     4561 gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcggc cagggtaacc
     4621 ttacagcagc attacaattt tgtaatgaac caggctgttt ctataacata tgatttatgg
     4681 catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca ggagtgggta
     4741 acgcaggggc tttccgaagc gggttatgtc attgatgccg tttctgatgg cagagatggg
     4801 ctttatcttg cgctgaagga tgattatgca ttgatcattc tggatattat gcttccgggt
     4861 atggatggct ggcagatctt acaaacgtta agaacagcaa agcaaacccc tgttatttgc
     4921 cttactgcaa gggattctgt cgatgacaga gtcagagggc tggacagtgg agcaaatgat
     4981 tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc acaattaagg
     5041 caacatcacg ctttgaattc aacattagaa atcagtggct taagaatgga ctctgtaagt
     5101 caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt tcagttactt
     5161 tggctactgg cctccagagc tggcgaaatt atacccagaa cggttattgc gagtgaaatt
     5221 tggggaatca actttgatag tgataccaat acggtggatg tcgccattcg caggctccgc
     5281 gcaaaagttg atgatccttt tcctgaaaag ctaatcgcca caatccgggg gatgggctat
     5341 tcattcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt ttaaccttgc
     5401 tttttatatt gctgctgtct gttgctggcg ctggaattgt ctggactctc tataatggcc
     5461 tggcaagtga gttgaaatgg cgcgatgata caacactcat taaccggaca gcacagatca
     5521 agcagttgtt aattgatggg gtaaatccag atacgttacc tgtatacttt aaccggatga
     5581 tggatgttag tcaggatatc ttgatcattc atggtgatag catcaataaa attgttaacc
     5641 ggacaaatgt cagtgatgac atgttaaata acatacctgc tagtgagaca atcagcgcag
     5701 ctggcattta cagaagcatt attaatgata ctgaggttga tgctttacga attaatattg
     5761 atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc agacataaca
     5821 tgcttgaaca gtataaaatt aatagcatta taatttgcat tgtcgccatt gtactttgct
     5881 cagtattaag tccgctgtta atcagaacgg gattacgaga gatcaaaaag ttgagtggtg
     5941 taacggaagc gctgaattat aacgatagcc gggagcctgt tgaggttagc gcattaccga
     6001 gagaactaaa acctcttggg caggcgttga ataaaatgca tcaagcctta gtcaaagatt
     6061 ttgaacgcct aagtcaattt gctgacgatc tcgctcatga acttagaacg cccattaatg
     6121 cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct gaatatcaaa
     6181 aaacaattgc cggtaacatt gaagagctgg aaaatatttc gcggttaaca gagaacatac
     6241 tgtttcttgc ccgggcagat aaaaacaatg ttttggtgaa actggactcg ctttctctca
     6301 ataaggaagt cgaaaatttg ttggattatc ttgaatacct ttcagacgag aaagagattt
     6361 gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttactg caacgaatgt
     6421 tatcgaatct tattgttaat gccattagat attcaccaga aaaatcgcat attcatataa
     6481 ccagttttct tgataccaac ggctatctta atattgatgt cgccagtcct ggaacgaaaa
     6541 ttcatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg cgtcattccg
     6601 taggtcaggg actaggcctt tctttagtca aagcgattgc cgaattacat gggggaagtg
     6661 ctacgtatca ctatctcaat aagcataatg tgttccggat tacgttaccg caaagaaatt
     6721 aacataaggc ttcagtacgc cataaattaa ccgtgaaaaa acgcagccac gtcgtgagta
     6781 ctaacgcggc tgcgattgat tagcgcttac attcaaacgt aacagggatt aacccgcgta
     6841 agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa aaggactgtc
     6901 gccagtgaga actttacggt ccttatgtac tcgcccggtg atgtcgtcat taataatgtt
     6961 catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agatgacccg gcatatagcc
     7021 tatctctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatggaat aaccattcag
     7081 tgggttatcg ccgtggcgaa gcgccagaaa agccgccggg ccgtggcaaa gggagataac
     7141 aaaacggtca tttttgattg cccactgcaa agcagcagct acgtcctggc tttctggtaa
     7201 accaataagt gcgccatgac caccaggaac aaagattgct gcatattcgc tatcagcgtt
     7261 gaggctggcg acaacatccg cgagtttctt cggattgcgg aacaacgatt tatgttgctc
     7321 aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa acttggtcat
     7381 cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat gatacaacgg
     7441 cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat cggttggcaa
     7501 ataacgttcg tccgcggcga tcaccagaat tttatgctta ccgcgatacg gttttggata
     7561 gtccacgcca tcaagatcag agacgggact ggtatattgg ctaagcgaat attctgaagg
     7621 gaagaatgca ttatcttcag caatatcgac ctgcggattt ttacttgttt gaacagtcat
     7681 agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact gagctggtcg
     7741 ctatttttaa ccagtctgga aaattgagtc aatatatcga aagcggtagt cggcgggtgt
     7801 aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa ttttccttat
     7861 tgagcaacgt cgtcgcaagt ggcctcaatg attttagacc attatcaaag gtacggggcg
     7921 gcggggagag atttagtgct gtgaaatatt gtggtactga tgaatattta tatcgcggat
     7981 tggtagagaa aagattattt catgcactat ttttgcaaat atttctatat gggaagcgtg
     8041 tttgttacgg ggtaattcat cagaatgtgc atttggggtg ttggctggaa gagtcgctgg
     8101 tagatgttat tgtggcagga ggggcagggt aaggtgctgt atcgatatgc tttggcaatt
     8161 gcgtgtgccg cagatggaga tgaaatgcca ataaaaaaag cctgagttcg atgagggacg
     8221 aactcaggct agtgttatgt tttacagtaa cacgccgcgg cgcgttggct gattagaact
     8281 ggtagacgat accaactgca aactggtcat cagttgcggc atcggtaatc gctgcatcgt
     8341 ctttatccag caggttaaat ttgtatgctg tgtagacgtt catgttctta ttgaagtagt
     8401 accaggtacc aacttcgata taatttacgc ggtcagcatc aacaccttta tgtccattac
     8461 gagcataaag atctttgcct ttggaatata cgtagcctaa agacggacgt aaaccgaagt
     8521 cgaactgata ctgtacaaca gcttcaaagt tttgggtttt atttgcaaaa tgatcattag
     8581 ccaaaacggt catgttgcgg gtttcaccgt acatcattgc tgcatagata ttatttgcat
     8641 catacttggc tgctaatgac cacacttcag cgttatcacc gtagccgtca ccagcttgtt
     8701 cattagtacg atcagatttg ctatatgcac ctacaaaacc aaaaccttcg atgttgtagt
     8761 ctacagacaa cgcatagcca tcaccgtttt gtttagtaac gccacgattg ctgttcttac
     8821 cttgatattg cacagcaaaa ttcagaccat caaccagacc aaagaaatca gagttacggt
     8881 aggttgcaac accgttggta cgaccgttca tgaagttgtc agcggaagcc caggaatcac
     8941 caccccattc aactaacata tcggtataag ctgcggcatc ataagcaaca ccgtagttac
     9001 gaccatagtc gaaagatcct aactcgttat atttcaaacc tgcgaaggtt aaacgggttt
     9061 tttcctgatt agagccttcg ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac
     9121 cagtcagttc gctggtgatt tgggtttcac ctttaacacc gaaacgagca taggaggtat
     9181 cttcgttttc gctgttatta tcatcagtat ttgaccagat gcgttcgcca accatcttgc
     9241 cgtagaaatc aactttattg ccatctttat tgtaaatttc agccgcattt gctgcgccag
     9301 caactaataa cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg
     9361 tgattataat cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gca
//
LOCUS       DAZGCS010000154         9331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGCS010000154 DAZGCS010000000
VERSION     DAZGCS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.156"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..134)
                     /locus_tag="ACHK01_004450"
     CDS             complement(<1..134)
                     /locus_tag="ACHK01_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099275.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     "
     gene            212..1306
                     /gene="ddlA"
                     /locus_tag="ACHK01_004451"
     CDS             212..1306
                     /gene="ddlA"
                     /locus_tag="ACHK01_004451"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8099276.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1330..1542)
                     /gene="yaiZ"
                     /locus_tag="ACHK01_004452"
     CDS             complement(1330..1542)
                     /gene="yaiZ"
                     /locus_tag="ACHK01_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO8099277.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1802..2110
                     /gene="yaiY"
                     /locus_tag="ACHK01_004453"
     CDS             1802..2110
                     /gene="yaiY"
                     /locus_tag="ACHK01_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO8099278.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2169..3263)
                     /gene="yaiW"
                     /locus_tag="ACHK01_004454"
     CDS             complement(2169..3263)
                     /gene="yaiW"
                     /locus_tag="ACHK01_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO8099279.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3276..4496)
                     /gene="sbmA"
                     /locus_tag="ACHK01_004455"
     CDS             complement(3276..4496)
                     /gene="sbmA"
                     /locus_tag="ACHK01_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO8099280.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGEWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4848..6005
                     /gene="ampH"
                     /locus_tag="ACHK01_004456"
     CDS             4848..6005
                     /gene="ampH"
                     /locus_tag="ACHK01_004456"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO8099281.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6006..6629)
                     /gene="iprA"
                     /locus_tag="ACHK01_004457"
     CDS             complement(6006..6629)
                     /gene="iprA"
                     /locus_tag="ACHK01_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO8099282.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6717..9331)
                     /locus_tag="ACHK01_004458"
                     /pseudo
     CDS             complement(6717..9331)
                     /locus_tag="ACHK01_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2422 a         2338 c         2201 g         2370 t
ORIGIN      
        1 tcaccgcatg gccatttaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc
       61 ttgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc tttgtcagca
      121 gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg
      181 cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt
      241 tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc
      301 cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca
      361 cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg
      421 cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc ttatcgacgc
      481 gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct
      541 gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc
      601 tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc tgctacgcga
      661 tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag
      721 ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg ctaatcaggg
      781 ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct
      841 ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga
      901 atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac
      961 cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt
     1021 tccggcagcc attgcaccag agatcaacga taagatccgg gcgattgccg ttcaggctta
     1081 tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga
     1141 agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta tgtatccgaa
     1201 gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga ttgagctggc
     1261 gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt ttaacgcccg
     1321 ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcaaccccgc
     1381 cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc
     1441 ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc
     1501 atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc cgttaacgaa
     1561 aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca ttttcgtaat
     1621 tgttaataaa tttaacttaa ggttgccatt ttgtgactca caacgaagat tcacgcttcg
     1681 ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa tgacctgtca
     1741 gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt
     1801 tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc
     1861 aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca
     1921 attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga
     1981 aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc
     2041 gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact ggcgacatcg
     2101 ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg catgacattc
     2161 ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag
     2221 gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg
     2281 cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt gtacagcgcc
     2341 gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc gctgtcgctc
     2401 atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ttctttactg
     2461 tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgactgcg
     2521 ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc aaagcggtat
     2581 atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa ccacaacccg
     2641 ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg ataccctttg
     2701 gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg cacaggatta
     2761 agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc aaaaatcgcg
     2821 ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt gccgtttggc
     2881 gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg ttcagcacga
     2941 cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc tgcctgataa
     3001 tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc
     3061 ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc tttcacgctc
     3121 gcaggcatct tctgccgcac gacgctcgcc acatctatcg ctttttcgcc ctttttcaac
     3181 ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa tgacagcgaa
     3241 gatagaggat tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc
     3301 tgaattttgt caccatccag ttcatgttca aagctgcgca gacgtttgta gatagacatc
     3361 aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca
     3421 aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac aattgacgga
     3481 aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg ggcgatgttg
     3541 aaatacatat agtgaaaata gaggcggaaa tagtttttcc gcacggcgct aaacagctcg
     3601 cgtaccgtag gcggcgtcgc acgcgtggca tcgtcttcac cataaaccag ctctttacgg
     3661 taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat ccctaccact
     3721 gccagcaatc cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga
     3781 atgtgcccga taatcggcag ctccggcaca tgcgtggaga gcgttaccag caccggcagg
     3841 aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc cagcgttgaa
     3901 gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggccc cttcgatatg acgcagttgt
     3961 tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac gtagtgactg
     4021 acaaagaagt tgttcagcac actgatcacc acagcgatca gcgcaatccc cagaaagacg
     4081 cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag cgcggtttga
     4141 atcagatcat agaacggtgc ataccaggcg ttgacggcga ccccgacttc caccaaaaac
     4201 caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca acggtgcggg
     4261 ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa
     4321 atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgcca
     4381 gtgatacgcg ccacccactc tcccccaccg gcttgccaga agataacggc aatcaatgcc
     4441 caaacaaagg ccgagagaaa aaacgctccc ggctttggga aaaaagactt aaacatatgc
     4501 actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccgctaca
     4561 aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc
     4621 ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac ataagcggat
     4681 taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc gtgtttacgc
     4741 gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt
     4801 agtataaata cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc
     4861 tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg gcgcagccga
     4921 tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat attttttacg
     4981 gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgca
     5041 gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg gtcgtgcgta
     5101 ttgcttccct caccaagcta atgaccagtg aaatgctggt gaaattgctc gaccagggga
     5161 ccgtgaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc gtgccaacct
     5221 acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc ctgccccgtg
     5281 aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc gagcaacgct
     5341 ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg gcgtactcta
     5401 accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc
     5461 agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc tacaccccct
     5521 caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca
     5581 cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg atgcgctgga
     5641 tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat cgcatgcaga
     5701 cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc cccggcaaag
     5761 cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt ccggggatta
     5821 ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga
     5881 atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa aatatgagcg
     5941 atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt atccccgcat
     6001 cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg
     6061 atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg cgatatgcgt
     6121 gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc attccagtca
     6181 atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat gagctgcacg
     6241 tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc tcgccagagt
     6301 tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc cgtacaattt
     6361 ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata
     6421 atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag agagataacg
     6481 ccctctaaaa taacgaaagt atcttcgtta ttgacatcac tggaaaatat aacttgcttt
     6541 tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag cttaccaaat
     6601 tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat gcccgtcaaa
     6661 tccttgacgg gcattattta gattaaatta ccagaatttc ttcggagtga agaatattac
     6721 caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc accaccgagg
     6781 tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca
     6841 acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg
     6901 ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg
     6961 ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac
     7021 tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg gaacagacca
     7081 gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc gtaaccggct
     7141 ttcaaaccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt accgttgctc
     7201 atggttgcag acaggtcgtt gttgaagtga gagtaactca agctaccatc aacaaagacg
     7261 ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga
     7321 ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac gatccactta
     7381 gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca
     7441 tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg
     7501 ccgttatcag ccaggccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc
     7561 aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc
     7621 agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata ggtgtatgca
     7681 cccaggtcag ctttgttagc agcagagaag gtcgcgttgg tgttgacgtc gtttacgtag
     7741 ataatttctt tatctttgta atcagcgata gcgccagcgc ctgttgcgtt gtcgatacga
     7801 ccgaactttg tagttaccgg ttgcagcaac aacgttgtca acagagctgt tatccagttc
     7861 gtcgccgttg ttcatgtcac cgttaccgtt gatagtcaga tgaccatcag agttcatagc
     7921 aacaataccg tagccgtagt tagacttgga agtatcccag gtacggtcgt tcaccaaatc
     7981 agcgttcaga acataatcgc tgctgtggat atcgaataca ccagacacta cgttgccgtt
     8041 atggtcagcg atgttgccgc tggtcagttc caaagtatca gtctggaacg gattatcatc
     8101 gaactggtct acattaatat ccagtaaacc accgttagtc acagttattg tatttgctaa
     8161 cagcgacgga gtgtaataac cattatagat acttgaattg tcatcactag acagcgctac
     8221 agtactgtag ctatcaactg tcagatgatc agttccaaca taaccttcat ctccaatgtt
     8281 caaagaagaa ccattggtca gagcgatgct atcagaaatc aagctagatt cagacacatt
     8341 aacctgagag ccattattca cagctaaagt atcaatgttt gaagcgttaa cggtattcca
     8401 ttcagaacca ttattcagag tgacgttaaa cacaccgctc tggaatactt gatcatcaat
     8461 cgtacgaccc caagcatttg cttctgttac aacgcccgga tacaggctat tacctgtaac
     8521 atcataccag tcagtcgaag cagtagattc atcatcaaca tttgctgatg tagtagctga
     8581 gcctacccac ttagaaccat tatccagagt gatattcagt tcatcaacat taagtgagtc
     8641 atcagcccag ccaccattgg tgtccagtac gccatcggca ttactgtcat gaccttgagg
     8701 atagaagttt gcgttgaaag tacttgcgaa aacaacatca cccagcagag tagaattgct
     8761 gaaataaaca ttgtttttca tggagtaatt tgaagccgca ctatcactga aatacagagc
     8821 tacatcatta gcttcgccgt taccagagta atcactaggt tccgcagagt taccatagaa
     8881 accactgtct tccagagtag ttacagaacc tgaagtcact gtggaatcgg taactgagat
     8941 attgttatcc caagtatgag attcagtagt gtaagtattg tttacggtat taccctgagt
     9001 caggctgata ccagcaacgc gggagttgtt agaaatattg atgttacttt cgacatcaag
     9061 attcactgcc agcccaaggc caacaccacc gtttgcaaac aaaccggcgt attcttcacc
     9121 ttcaccagct ggggtatgga aggtttcgtt agtatatttg gtaacgtcac cgtccagata
     9181 tgaatcagtg aagtataaac cttcataatc gtcatcaatt gtagaattag caatatttaa
     9241 agtgaataca tcaccatcat gccaattatc atcaattaca cgatcagtac cgtattcaga
     9301 gaactgatag acataatgac catcactgct t
//
LOCUS       DAZGCS010000155         9218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGCS010000155 DAZGCS010000000
VERSION     DAZGCS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.157"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            246..695
                     /locus_tag="ACHK01_004459"
     CDS             246..695
                     /locus_tag="ACHK01_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099283.1"
                     /translation="MQLLSLRTIDGDTLYLKFRNVITEAFLDTPTKSYDFFGDVVLSQ
                     CINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILI
                     IIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYMIFALNK"
     gene            773..1987
                     /locus_tag="ACHK01_004460"
     CDS             773..1987
                     /locus_tag="ACHK01_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099284.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRTALLSL
                     NQPFTQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            2044..5370
                     /locus_tag="ACHK01_004461"
     CDS             2044..5370
                     /locus_tag="ACHK01_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8099285.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRY
                     ENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARHEEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            5363..6973
                     /gene="tssA"
                     /locus_tag="ACHK01_004462"
     CDS             5363..6973
                     /gene="tssA"
                     /locus_tag="ACHK01_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8099286.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALSQLTVSFTAWPEDQHSPSLMLLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDDTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            6979..8397
                     /locus_tag="ACHK01_004463"
     CDS             6979..8397
                     /locus_tag="ACHK01_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8099287.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIGGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGSEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            8390..8812
                     /locus_tag="ACHK01_004464"
     CDS             8390..8812
                     /locus_tag="ACHK01_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099288.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQDICP"
     gene            8892..>9218
                     /locus_tag="ACHK01_004465"
     CDS             8892..>9218
                     /locus_tag="ACHK01_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099289.1"
                     /translation="MIRLIFSFLLCTLCAGCLSQPFYSCSIVMVNTCKVSINISTVNN
                     STHYDGKVRDNFHLDIGERKPIVYFIYTGRESDVFNKNPVNIVSELSILISDGNNSKT
                     LSGYHIS"
BASE COUNT         2296 a         2275 c         2553 g         2094 t
ORIGIN      
        1 ctggatagat aaagaagcaa caattgagga ggcatatcgt ggaacagatg aagcatacta
       61 ttctgttata aaaccttgcg ctgaaaaact ggctaaagat cctgataaca tacaaatact
      121 aatggaggga ataggaagat agtatgcgca ttatgttaat attactttgt ttttttcttt
      181 cccctgtttt tcaggcgata agattaaaat cgtgaaaacc tgtcaactga aaaaaaaatg
      241 aaagtgtgca gttgctttcc cttagaacca ttgacggtga tacattatat ctaaaattca
      301 gaaatgttat caccgaggcc tttttagata ctccaactaa aagttatgat ttttttggag
      361 atgtggttct ttctcagtgc ataaatggtt ctcttatctt tgcgctggaa tatggttctc
      421 catatataaa aggttgtctg gtaactggtt gggaaaatgg gtttaaaggt aagaactctc
      481 ctgaaggctt ttgtttcgca gagagaaata ttcccgaagc ggtatggttt ggaaaaaaca
      541 atattttaat tatcatccga aaccaaatgg atgttgggac atggaaaggg aaatatatta
      601 tctatgataa tgcgaaaaag tcaggagaaa gagcatactc atcagataat ctaccatcaa
      661 aagatggtta catgattttt gcattaaata aataaaatga ctctatagat aactttaaat
      721 ttcacttggt tattaacttc tgtccgataa tgcaaaataa agggatgtac tgatgcctgt
      781 ccgacttaat accattcccg acgcagaatc ctatcccgca ccgccagccc gcattaaatg
      841 gctgtcagcc ttattactga tgctgtctgt tggtgtggca ataacctcat tattcgccag
      901 cgatgaactg gcaaaaaacg gcccgcattt ctgggggctg gcctgcggtg tgcctgcctt
      961 tatctggtcg ctggttgctt cagttcgctg gttgtttttt atcacgcagt acattcgggc
     1021 tgatgcctgg aataagcgcc gggaagaggt gattttgcag gaaacgcgac ggggacgacg
     1081 ggcgttgcag atacttagtt tttccgtaca aaccgcgctg aatggtgatt ccgttgcaga
     1141 aactacaaca gcatttctgg cgcgtcagca ggtgttgaat acttacgctg atttgcgcgg
     1201 agaagatact gtgcgtcgca gtgtcattcc cgtattaacc aataaaccgg ttaccgggcg
     1261 tttatccgaa attatttccc gcctttttat tgatatcaga ccgcaattgt ttctgctgcc
     1321 ccctgatttt catatcaacg tactactgga aattgatgcg ccgctttcag gggcctccgt
     1381 gagaactatc tggcaggacg aatggcaaaa agccggactc ccggaagccc gtctgttttc
     1441 tgccccggag ccggggctgg ccgccgtgga cgactggctg gacaacttta ttcaggaaaa
     1501 ggccgtactg ctggtgattt ccgtgtatct ggaaccgaag actccggaac ggacggcaga
     1561 atcctccacc gcgctgttgc tggcaaaccg gttgacgcaa accgcgctga cgccgctggc
     1621 cctgctgcat cgtcctgaac gtatcacaga tacagagatg atggcgtcag gcattgcaca
     1681 ggcgctggac tggatgccgg tgcagccaga tgccatatcc ggcatatgga ctgccgaact
     1741 ggacagggaa cagcgtaccg cgttgttatc ccttaaccag ccgtttactc aggaagccct
     1801 gatgtacgag ctggatgcct ttctggggcg ctccggaccg gcagcgccgt ggctgtccgt
     1861 cgctgcggca acgctggcgg caatacaatc acaacacccc caactaacgc tcagcggcgt
     1921 tcagggcggg cattattcat gggcgaccgt ggtgtcgcca ttcgtttccc cacaggaggc
     1981 atcatagtgg ttccgggatt aaaccgtctc cggccccgtt ctgtttcagg gcctgccatc
     2041 gtcatgaccg tggctgttgt ggttgcgctt tgcacgttta ttcaccgtaa accggcattg
     2101 ctgggcgttg aaccgggtag ctcacagcaa atgtactgga tggctggcgt gattgccgca
     2161 ggcgtggtct ttttgattat catgggggtt tatcatctgc tgaaccgcca cgctgccgta
     2221 caaaactttg aactccgcag acaacttgcc gccggagatg actatcgtgc ggtgacgaca
     2281 aagaaagccg acagccgtca tccggcaatg gtggaactcg ccgccttcct gcgcgaccgc
     2341 tacggtccgt tctggcggcg taacgtccgc ctgctgctgg tgaccggcga gcctgaacag
     2401 gcagaagcca tcgcgccggg gctgaccggg caacactggc tggagggcga ccacacggtg
     2461 ctgatatatg gcggcaggcc atcagcggag cctgatgtca cactgctgac cgccttaaaa
     2521 aaactgcgcc gcagccgtcc gctggacggc atcatctggg cgctgacaga agaacagagc
     2581 cgccagacag cgcaactcga caaaggctgg cgcgaactga taaacggcgg taagcgactc
     2641 ggttttcagg ccccactcta tttgtggcag gtctgtgaca acggtgatta tcaggccgga
     2701 cgccccctgc aaagcgtcgg ctgcctgctg ccggaacgct gcaccccgga acaactgacc
     2761 gccatgctgg aagctcacac cctgccgctg acggaacagg gcatgtcgca actgctggct
     2821 gataaccgcc atgattttct gctgcgtctg gcccataccc ttgcagagcg gggtatcgct
     2881 cactggcaaa gcgtcctgaa accgctgctg gcaggcggcg cattctcttc cctgcgcctg
     2941 cgtggtctga tgttcagccc gccgcttacc gccgtaccgg aggccgcacc tcatgcgtgg
     3001 ctgccgtcac aggtctgggc gggcgtgacc ggcgataacg cacgggggcg tgcagtgggc
     3061 tttccgtggc tacgcgccgc gctgatgtcc gctgtctgcg tgctggcaat atggggggcc
     3121 ggaatgacga cctctttctt cgccaactgc gctcttgttc aggaaaccgg tatccagacg
     3181 gcgcgggcgc tcgatacccg cctgccgctg gcagaacaac tggtggcgct gcataccctg
     3241 cagggcgaac tggaacgcct gcaatatcgt atccgcgaag gtgcgccgtg gtatcagcgt
     3301 tttggccttg aacgtaacca acaactgctc gccgccgcct ttcccggcta tgcgcaggcg
     3361 gcaaaccggc tggtgcgcga cgtggccgtt gaccatctgc aacagcaact gaacgccttt
     3421 gtcgccctgc cgcccaacag tcctcagcgt accgccaccg gtgaacaacg ctataagcag
     3481 cttaaggcat tgctgatgac ttcccgcccg gaaaaggccg acgctgcctt tttcagtacc
     3541 acgctgatgg cagacagcct gcgctacgag aatatcccgg aaggtgtgca gcagagcgtg
     3601 tttccgtcac tgctgacctt ctggatggcg aacctgccgg aacacccgca gtggaaaacc
     3661 tcgccgccac cggaactgac cggcgcggtg cgtaaaatcc tgctgcgcca gattggtgtg
     3721 cgtaatgccg aaaacactct ctaccagaac gtactgcaac aggtgtcccg caactacgcc
     3781 gatatgacgc tggcggacat gaccggggat accctcaccg aatctctttt cagtacggaa
     3841 cagacggtgc cggggatgtt cacccgtcag gcgtgggaag gacaggtcag ggaagccatc
     3901 gagcaggtgg tgacggcgcg gcacgaggaa atcgactggg tgctcagcga ccgccagcag
     3961 gatacctctg cggatatatc gccggatacg ctgcgtaacc gtctcacctc acgctacttt
     4021 accgacttca ccggaagctg gctggcgttt ctcaacagca ttcgctggaa aaaggaagac
     4081 tcgctctccg gcattctcga ccagctgaca ctgatggccg atgcccgtca gtcgccgctg
     4141 attgcgctga ccgataccct cgcctggcag gcggcggcag gcagggaaaa ccgtggcctg
     4201 tcagactcgc tggcgaaatc ggcacaggaa ctgtttaacg gcaaggagaa aacgccgcag
     4261 caatcccgtg aaggcgacgt gcctgtcggg ccgctggata aaaccttcgc tccgctgctg
     4321 cggttgctgg gcgataaagc cggaggcggc gacagccagc tgagtctgca gacctacctc
     4381 acccgcgtta cccgtgtgcg tctcaaactg caacaggtga ccaacgcccc cgacccgcag
     4441 gaaatgacgc aacagctggc acagacggta ctgcagggca aaaccgttga cctcaccgac
     4501 acccgcgact acggacggtt aatcgccgcc agtctgggcg aggaatggag tggcttcggt
     4561 caggcgctgt tcgtccgccc ggtggagcag tcgtggcggc aggtgctgac gcctgcggcg
     4621 gacagcctga accgccagtg gcagcgggcg attgtcagcc actggaatca ggacttcgct
     4681 ggtcgctacc cgttcaaagc ctcacagaac gatgcctccc tccccctgct ggcgcagtat
     4741 ctgcgcgatg acgggcgcat caaccagttt attgccgcca acctttccgg cgttctgaaa
     4801 cgtgaaggcc gctactgggt ggcggacgcc atgaacacgc aggggctgac ggtcaacccg
     4861 gactttatcc gcgccctgaa ccgcctgcgc gacgtggctg acaccgcctt tgccagcggc
     4921 gatgccggaa tacattttga actgcaggca aaaccggccc gagacgtgat gaagacacat
     4981 ctggtgattg acgggcagga actggagtat ttcaaccaga aagaacgctg gcagcgtttc
     5041 aactggccgg atgaacagtg gcaacccggc gcatcgctga gctggaccag tacgcaggcg
     5101 atggaacgca tactggcgga ttaccggggg agctggagcc ttattcgcct gctggaacag
     5161 gcgcaggtga caccggtgga cagcagcacc tttaaagtgg tgtggaaagc gcaggacggc
     5221 ctgccgctga attacctgct gcgggttgaa cagggtaaag ggccgctggc gctgctggaa
     5281 ctgaaaaact tccgcctgcc gggacaggta tttctgaccg gcaggagtat gaaagatgcg
     5341 gaagaatacg gggaagacgc cgatgagtga actgttgcaa aagctgaccc gtagctgttt
     5401 tgtcgaccgg gatgcactgg atgtggcgcg aacacaggca gcgctgtggc agacatggct
     5461 gctgccggtg acggcaaata cgccggtcgg ggaagacccc ggctaccacg acgatttcct
     5521 gcgtatccgc gacgagatga acaaactttc cggcgcggat acggacctca tctgccagct
     5581 tgccgaatcc ctgctgctga cgcaggcaaa agacgtgcgc atcgccactt actacatctg
     5641 ggcgcgactg caccgcgacg gtgagcgcgg actggctgag gggctggcgc tactgaccgg
     5701 actggtggaa cgcttcggta cgcaactgct gccctcgcga ccggccagcc ggaaaatggc
     5761 gctggagtgg ctggcagggg aaaagatgct cgacagtctt gcccgctacc cggaagtggc
     5821 aaaagaggat tttgcgaaca ttgttgctgc cctcagtcag ttgacggttt ccttcactgc
     5881 atggcccgaa gaccagcatt ccccctccct gatgctgctg attaacgcgc tggaatcacg
     5941 tctggcgcag tcgggcggga tgaacgcggt ggtgccgcaa aacagcagca gcgttcctgc
     6001 accgtcatcc ccggtggatg cgccacaggt acagaccatc acctccgggc gtgacctgct
     6061 cgaccaggcg aaagtgctgg cccgttatct gaacgaacag ccgcagggct ggctttcggc
     6121 gcaccggctg atgaaaaccc tgcgctggga cacagtgcat gaactgccgc cggatgtgga
     6181 cggcaaaacc cggctggccc cgccgcgcac cgagtcacgc aaccagctta agcggctgta
     6241 tgcgcagcag aactggacgg aactgctgga gcaggccgac ctgatgttca gcaccggcgt
     6301 cagccatttc tggctggata tccagtggta tctgcatcag gcgctgacta aggcgggcgc
     6361 accgtgggac aggtggacag ccgttatccg tcaggacctg gcgctgctgc tggaacgtct
     6421 gccagggctg gaaaatctgg catggaacga cgacacaccg ttcgccgacg aggtgacgcg
     6481 caactggata gcgcagcagg tcatgatgcg ggaagacggc gcatggcttg cagggaaagc
     6541 cgctgtaccg acagacgacg ccacaaatga cgtactggcg ctggagccgg aagcactgga
     6601 gatggcagac agtcagggcg tggaagcggc tctgggctgg atacagaccc gtccgggcat
     6661 caccacggca cgccagcgcc tgttattgcg actgctgatg gcgcgggtgg cggaacagta
     6721 cggtaaaaac gaaatggcgc tcctgctgct ggaagaactg gataccgccg cacaggggct
     6781 gacactgacg cagtgggagc cggacctgct gtttgaggta aaagcccgac aactgaaact
     6841 gctgcgcctg cgggcgcacc gctatgcgga taaagccctg ctgaaccgaa aaatggaaat
     6901 cctgctggga acgctggtga caatcgatcc ggtacgggca gcggtattgt gtgatacgca
     6961 acataaggac tgatggcaat gggaacaggc tactttctgg tcagggggga caaaaccacc
     7021 tgcggcggga agattattgg aggcgcggac gaccatacga tcatgggtat cccacaggca
     7081 cgggatatgg accgggtcac ctgcggcaga tatccgggaa tgtttattat tgtgggcggc
     7141 gttcctgaaa cagatattca cggcaggctg atggcgggga cgctggacag ccagagcagt
     7201 tgcccctgta aggcacggtt tattgcgtcg atgatggatg atacgtatga gacggatgac
     7261 gggggaagtg aagcggaaca acatgcgcag tcggcgaaaa aggatctgac ttctggttct
     7321 gattcatcct ctgatgatgt aaaactggat tacagaatta aattatcagg taataagata
     7381 ctgacgccac tgaatatacc tgattataaa gaaatgataa gcggtggtag tacgaagaat
     7441 acagaaaaaa tagattttac tatcacaaat aaaggcgatg aggctgaagg actttctctg
     7501 gaagtgcttg atggtaatga agtgatttac agtgaacagc agaccggtaa atattttgat
     7561 aagggagagc atgcctggca atgggatggc tacagtaatc aaggcatact ggatacaact
     7621 aagctaaaaa gcaaatcact actcgtaaga ctgattgctt tatgtggtga tatgatgata
     7681 aaagttgatt atccacttca taattcacca gaggaacaaa aatgggtcga tgtaaaggta
     7741 gacagaaaac aaaaaacagt agatataata tggcgactgg cagttagtga tggagggata
     7801 aaaggaagta atcctaagtt atccccagtt ccttataacg acttggttaa cctgaccaaa
     7861 aatggagttg aattttattg gtctcgaaat ggaagtcgcg gcggggggat tggtgaaaat
     7921 attgttacag cgattggtgt ttttaaagta aatgtaaagg ctgagataaa tattactcca
     7981 tccatgcgaa ctttttcttt gatatcatca ttagatcctg actttcaggc atccgttagt
     8041 ctatcaggat ttgaaaaaat atattataac tacggtgata gttataagga tattcaagat
     8101 gaattacagg cgcttcttga cgctaataac agatataaat gggatagtgc tcatgaaatg
     8161 gggcataaag tactcgatga atatggagag ggttctagcc ctgattattc gtggactcat
     8221 aaaggaacat ctactcttat gcaaaaaaca ataccaggga atgttatgcc cgctcaggga
     8281 gagattgatg taatgaaata tggaaaatat cgtcctgaca tgtatactcg attggttgca
     8341 gcagatgaag atgttcaggg attgatctgg ttaagcagga taaaatttga tgactagagt
     8401 agttattgct ttattatttc ttgccggatg tacttcgcaa atgaagtatt ccaatgtcgt
     8461 caggattagg aatgtatgtt cctcagatat taatgtgttt attcctgagt atgtttttgg
     8521 aggtaatgtt attaagcaac atcatattta cttacctgtt aatgggattg atgttttgtc
     8581 ttatttttat actgaaaaca tcaatgatgg tttccaatat gaagaagcat tcaccgtaaa
     8641 taaagatagc tcgggtaatt ttgattatcc gtttgttatc ttatcatcag gacgtgaaaa
     8701 aaaatttggg aaaaacgatc taattgaatt atccagggac atttcaacaa aagacgataa
     8761 gaagcatggg gttgtaaagt acatgataga tagtcaggat atttgcccat agtatgattc
     8821 atatcataat caactgaaaa aatgacatta ttaatcattt gtgatttttt gatactacca
     8881 agtgaaattt gatgattaga ttaatattct cttttttatt atgcacactg tgtgctggat
     8941 gtttatctca accattctat tcttgttcca ttgtcatggt aaatacatgc aaagtatcta
     9001 ttaacatttc gacagtcaat aattcaacac attatgatgg taaggtaaga gataattttc
     9061 atcttgatat cggagagcgg aagccaattg tatactttat ttacacaggt agagagtcag
     9121 atgtttttaa taaaaaccct gtaaatattg tttctgaact tagtattttg atatctgatg
     9181 gtaacaattc taaaacttta tctggatatc atatctcc
//
LOCUS       DAZGCS010000156         8847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGCS010000156 DAZGCS010000000
VERSION     DAZGCS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.158"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..769
                     /gene="yneK"
                     /locus_tag="ACHK01_004466"
                     /pseudo
     CDS             1..769
                     /gene="yneK"
                     /locus_tag="ACHK01_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
     gene            919..2109
                     /gene="ydeA"
                     /locus_tag="ACHK01_004467"
     CDS             919..2109
                     /gene="ydeA"
                     /locus_tag="ACHK01_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO8099290.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2134..2799)
                     /gene="marC"
                     /locus_tag="ACHK01_004468"
     CDS             complement(2134..2799)
                     /gene="marC"
                     /locus_tag="ACHK01_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO8099291.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3011..3445
                     /gene="marR"
                     /locus_tag="ACHK01_004469"
     CDS             3011..3445
                     /gene="marR"
                     /locus_tag="ACHK01_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO8099292.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKKLTADEVATLEHLLKKVLP"
     gene            3465..3848
                     /gene="marA"
                     /locus_tag="ACHK01_004470"
     CDS             3465..3848
                     /gene="marA"
                     /locus_tag="ACHK01_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO8099293.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3880..4098
                     /gene="marB"
                     /locus_tag="ACHK01_004471"
     CDS             3880..4098
                     /gene="marB"
                     /locus_tag="ACHK01_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO8099294.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4155..5594)
                     /locus_tag="ACHK01_004472"
     CDS             complement(4155..5594)
                     /locus_tag="ACHK01_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8099295.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHTRG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5619..7292)
                     /locus_tag="ACHK01_004473"
     CDS             complement(5619..7292)
                     /locus_tag="ACHK01_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO8099296.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7348..7659)
                     /locus_tag="ACHK01_004474"
     CDS             complement(7348..7659)
                     /locus_tag="ACHK01_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO8099297.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7687..>8847)
                     /locus_tag="ACHK01_004475"
     CDS             complement(7687..>8847)
                     /locus_tag="ACHK01_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO8099298.1"
                     /translation="ILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIA
                     FLVARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIV
                     ALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISP
                     AGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEAN
                     LENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLA
                     KMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAV
                     PWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         2402 a         2124 c         1899 g         2422 t
ORIGIN      
        1 ataattttcg ccatgatgga gaaattaata tttttgcgct cccaacccaa agcctgtcac
       61 tatggggcgc gtatttcaga ccggcgtcga gcgtgtgcta tttctgtttc tgaatgattt
      121 tattgaacaa tttccaatgt tcaaccttgg cgttcccatc aaaagagcgc atacaccaca
      181 tattgaaccc ctgcctccgg atcgccatac cgctgcagat tacttacgcc agtttgattt
      241 gcttgtcctg aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa
      301 ctctgaggtt cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga
      361 cataacggac agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc
      421 taacaaacat gtactacccg aagtctgcat ctgcttttta tcccttttag cagaacaaga
      481 atctcctcat tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca
      541 atttatgaat cccaatgaaa gtgatttgaa tgacgtgttg atgccagcat cattaagtga
      601 tgataaaatt atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac
      661 gccgccaaaa gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc
      721 acctataccc gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt
      781 accgactctc tgacgcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc
      841 acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa
      901 ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg
      961 gtcgttacgc tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc
     1021 ctgctctctg acattgcgca aagttttcac atgcaaaccg ctcaggtcgg cattatgttg
     1081 accatttatg catgggtagt ggcgctaatg tcattgcctt ttatgttaat gaccagccag
     1141 gttgaacggc gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg
     1201 tcgtttttat cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca
     1261 catgcgattt tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag
     1321 cgggcacagg ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta
     1381 cctctcgggc gcattgtggg ccagtatttc ggttggcgaa tgaccttctt cgcgattggt
     1441 attggggcgc ttatcaccct tttgtgcctg attaagttac ttcccttact gcccagtgag
     1501 cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt
     1561 tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct
     1621 tttgtgcaaa acattgcggg attcagcgcc aactttgcca cggcattact gttattactc
     1681 ggtggttcgg gcattattgg cagcgtgatt ttcggtaaac tgggtaatca gtatgcgtct
     1741 gcgttggtga gtacggcgat tgcgctgttg ctggtgtgcc tggcattgct gttacctgcg
     1801 gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc
     1861 atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg
     1921 atggcgctat tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat
     1981 caggtgagtt tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt
     2041 gccgcgttaa tttggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag
     2101 acgcaatagt tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag
     2161 gatgccgtta ataataaact gcacacccat acataccagc aggaatccca tcagacggga
     2221 gatcgcttca atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct
     2281 tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca
     2341 atcagcgaag gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt
     2401 tcccggacca gcggtacttg gcattgctag cggcacaaag gcaatattgg cactgggttc
     2461 atcttccagc tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg
     2521 aaagagcatc cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg
     2581 aatcgaaatg ccaaatgtat ccatcaccag ttgcccggcg taatacgcca ccatcatgat
     2641 ggcaaatacg tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt
     2701 gcctgccagg ccaagaaata acgcgacagt tgttaatggg ttagctaacg gcagtaacac
     2761 caccagcccc aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta
     2821 tctgggttat cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta
     2881 accttgcatc gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt
     2941 cattcatttg acttatactt gcctaggcaa tattatcccc tgcaactaat tacttgccag
     3001 ggcaactaat gtgaaaagta ccagcgatct gttcaatgaa attattccat tgggtcgctt
     3061 aatccatatg gttaatcaga agaaagatcg tctgcttaac gagtatctgt ctccgctgga
     3121 tattaccgcg gcacagttta aggtgctctg ctctatccgc tgcgcggcgt gtattactcc
     3181 ggttgaactg aaaaaagtgt tgtcggtcga cctgggagca ctcacccgta tgctggatcg
     3241 cctggtctgt aaaggctggg ttgaaaggtt gccgaacccg aatgataagc gcggcgtact
     3301 ggtaaaactt accaccagcg gcgcggcaat atgcgaacaa tgccatcaat tagttggcca
     3361 ggacctgcat caagaattaa caaaaaaact gacggcggac gaagtggcaa cacttgagca
     3421 tttgcttaag aaagtcctgc cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata
     3481 ctgacgctat taccattcat agcattttgg actggatcga ggacaacctg gaatcgccac
     3541 tgtcactgga gaaagtgtca gagcgttcag gttactctaa atggcacctg caacggatgt
     3601 ttaaaaaaga aaccggtcat tcattaggcc aatacatccg cagccgtaag atgacggaaa
     3661 tcgcgcaaaa gctgaaggaa agtaacgagc cgatactcta tctggcagaa cgatatggct
     3721 tcgagtcgca acaaactctg acccgaacct tcaaaaatta ctttgatgtt ccgccgcata
     3781 aataccggat gaccaatatg cagggcgaat cgcgcttttt acatccatta aatcattaca
     3841 acagctagtt gataacgtga caacgtcact gaggcaatca tgaaaccact ttcatccgca
     3901 atagcagctg cgcttattct cttttccgcg cagggcgttg cggaacaaac cacgcagcca
     3961 gttgttactt cttgtgccaa tgtcgtggtt gttcccccat cgcaggaaca accaccgttt
     4021 gatttaaatc acatgggtac tggcagtgat aagtcggatg cgctcggcgt gccctattat
     4081 aatcaacacg ctatgtagtt attgtcaggc tctgatcaca gagcctgaca tctccgtaat
     4141 atcttcctca agtctcaaat attttcgcca ttgttggcga tgagatcccg ataccagtaa
     4201 aacgacgctt tacgtatccg ttccagcgtt cccttccctt cgttgtcttt atcgacataa
     4261 atcattccgt agcgcttttt catttcccct gttccggcag aaaccaggtc aatgcagccc
     4321 cacggggtgt agccaattaa gtcaacacca tcttcaacta cggctttttt catttcccga
     4381 atatgggaag caaagtaatc aatgcgatag tgatcgttca ccgtgccgtc agcttgtctc
     4441 tggtcaaccg caccaaatcc attttcgaca ataaacagcg gcaactggaa atgatcccag
     4501 aaccagttga gtgaataacg tagccctgcc ggatcaattt gccatcccca ttcggatgta
     4561 tcgatataag ggttgctgac caaatctcgt ggttcaacat aatccagttg cgggttatcg
     4621 ggcgaaaatt gtgtcgtaaa agacatgtag tagctaaagc cgataaagtc gacacaacca
     4681 ctggcaagaa tcgcgttatc ttctggtgtg atatcgaggt tgaatccttt cctggcaaag
     4741 taattcagca tatgttgcgg ataatatcca cgagtatgaa catcagtaaa ccagtaacga
     4801 cgatgcattg ctttcgtggc catcatcata tcgttgggtg cacacgtcag aggatagatg
     4861 ggacacatgg cgatcataca gccaatgtta aattcaggat tgatcgattt tccagtctgt
     4921 accgccaggg cactggcaac taactcgtaa tgtaccgcct ggtacattat ttgttcgcgc
     4981 tcattgatat cttcctctgg cgaatacaag ataccggaat tagtaaatgg acacaggctt
     5041 tcgctgaaat tcacctgatt attgatttcg ttaaacgtca tccagtactt tactttttct
     5101 ttatagcgcg tgaagaccgt tgatgcgaag cggataaaaa agtcgatcag tttacggttt
     5161 cgccagccac catattttgt caccagatga taaggcatct caaaatgtga aagcgtcacc
     5221 acaggttcca ttccctgctt aaggcattca tcgaacagat catcataaaa ttgtaaaccc
     5281 tcttcattcg gctcctgttc gtcaccttgc ggaaagattc gtgtccaggc aatggaagtt
     5341 cgaaagcatt tgaatcccat ctcggcaaat aactgaatat ctgttttata gcgatgataa
     5401 aaatcaattg cttcatgatt gggataatta agcccgtcga taacgccttc tgtcacttca
     5461 cgcggcaccc cgtgagcgcc agcagtcatc acatcagcga tgctgatgcc ttttcctcct
     5521 tcattccagc caccttccaa ctgatgcgcg gctaccgcgc cgccccataa aaaacctttt
     5581 ttaaatcctg acatacacaa ctccttaact aaaatagatt accaccagac ttccgcctgg
     5641 atgccggcca taaactcgtc tttgctgttc tcattaaact taaaattcga taattcgtta
     5701 tccagaatat tgatatacgt tccatagaag cgaatttctg gtcgtgaacc taaaatgctt
     5761 tcacccactt tcaatgcatg ccagagtgtc gttttataag ccgattcatt tagtgttaca
     5821 ccctgctgag ttttgttctg ttgcttaaac cagcctaatt caagccccgt ctgattccat
     5881 gtattccaga tccaggccgg tcttattaca gtgcgaatac tgtcaaaatc actatgagca
     5941 ccactttcat aactataaac atcttcgccg tgagaataga caagcgcgtt agccataata
     6001 atattgtcag taagatacat ctcgccctga gagattaaac gccaggcgat cccattggta
     6061 tgatcaccat agtaatagcg accatgcgcc atcgtattac tggcatctga gaaactggca
     6121 aaactgctgg cataggaatt attggcaacc tgtaacgtaa attcattaaa cgtatcgcgt
     6181 tgcaggtttt gccgtaaaac agaagtcagc atccagctat ctttcatttc aaaataactg
     6241 ttgtcatttt cgttatcttg ttgttgatcc gttttattag gtgcggaata tttagccatt
     6301 aatgataatg tcgcatcatc ccataacggg atattgcgat aacgaacatc cacagaattg
     6361 gtattcatct ggctggtacg cgaaaaatca cgagagtaaa catcgacatc atcacggctt
     6421 aaggacatat caaacagccc tacaccaagt gcccagtttt caatccccac acccgcggcg
     6481 acatccgtgg ttaaggtttt ccagtccagc atctggatct catattgcgg gagtttatgt
     6541 ttgccgaccc agaagtctgc ctctggtgcg aagggtaaaa aacctcgcgt tgtcaggtaa
     6601 atatcgctga actgcatgat attttcattg gcactgtcac caaaccaggc atcgttatac
     6661 tgctcaccca cgttgccatc ataggtaacg accgcatttg ccgttttacc gtcctgatta
     6721 taaacacgct gatttaaggt caggtcaaac caaccgctca tctcattacc aaaacgtccc
     6781 agagaacccg ctgcataagt ttgtggtgag ccgtgattac cagtacccca gcctgagcgg
     6841 aagtacccct gatagctgaa cccaatatca tcctttatat atttactgat atctttcagg
     6901 gtcacgcttt cgatagtggt tttgtcgcct ttactttcaa taacggcaac ttgctgcgat
     6961 ccatttatag tatgcgctgc ggtaaacgag ttctgttcac cgttttcatt tttgatgtta
     7021 tcagcgacgg gagatgtcgc tgatatttca gcaagcgagg ctgatttata tttgttttcc
     7081 gtgatgcttt tttgtaaggt cgaaagtcgg gatttatata ctcccagttc attctgtgtt
     7141 gctttcagct cttgtttatt ttgcgacaat tcattttcaa gcaattcaag gcgctgctct
     7201 atggttaatt ttgttgccat cgcgttcaat ggaaacaatg ctgtaataat agaaaaagac
     7261 aaaaggctga cgttaagcgt ctttatattc atgacttatc cttaagagta taataatttc
     7321 ctccagctat cgcactggag gaaaaaatca ggcatggagt tcttttctta atgaaataat
     7381 ttccctggct aaatcctggc ataatattgc attcatcaaa tgatcctgtg catgaatcag
     7441 aattaaatta acttttattt tgccacagcc ttcatcctga ctaattaatg cggtctgaat
     7501 cttatgtgcc tgaagacagg cagattcaga actggccatt agctgagttg cgccctgcca
     7561 gtctttttgc ccggcacaac gtatggcttc catcgcgtca gaacgtgcct gccccgcgtt
     7621 gaccagtaac tccataacaa gttcttcatc tgcaaacatc acaatctcct tcctgggtaa
     7681 atggaattat ccgttagttg tcatgttatt gactttgttg gcggcaataa caaaaggtaa
     7741 atagatcatt gtcgccacac ctaaacataa aaaaccaacc agtaaagcca gccagttccc
     7801 cccggttccg aggaaagcaa ttaaaggtcc gggcgtagtc caaggcacgg cataagcaac
     7861 cggtggaata atttccatcg aaacaaagaa gtaaccaatg gcacagttaa caatgggaac
     7921 cataataaac ggcaccatca tgatggggtt aagtacaata ggaaggccaa acattatcgg
     7981 ttcattgata ttgaaaatac cggggataaa tgacattttt gccagatcac ggtattccgc
     8041 acgcttagaa gcgataaaaa tagccaacaa taaccccaac gtcatacctg aaccgccgca
     8101 gttggcgaat gcatcattaa tagaagtcca ggtaatcgga tatggcgcgc cccaggtagt
     8161 gccatgttga gcggcccagg agagattctc cagattagcc tcagaaaaaa cggtttctcg
     8221 aattgccgat gtggtattag gtccgtggat ccccagcacc cacaggaaat tacccacagc
     8281 ccccaggata attaccgcaa agatattcgt tcccatatgt ttgagcggcg tctggattaa
     8341 tgtgtaaata agatcgttta gtcctgccgg ggatatcagt gtcaggcaat aattaagtgc
     8401 ggaaaagaac accatgacaa aaaatattgg caataaaact ttaaatgaac gcgctaccgc
     8461 aggcggtaca gctgccggca tcgttatggt gatttttggg tttcgagcta agcgacaaaa
     8521 tatttcactg gtgatcaatg caacgataac agcaacaaac aacccctgcg cgccgagata
     8581 tttggtcgtc aggaatgctt gcccatctat catctgatat ggcgtataaa caataaaaaa
     8641 tgctcctatt gccgttaatc cgcataaaag atcatcttgc tcatagctaa tcgccatatt
     8701 cctggcgacc aggaaagcaa tcataattga catgatattt acagaaccat tcattaccgg
     8761 agtaaaaata gcttgtgctt tttcaaggtt ggggaaaatg ctattcagat gcagtaatcc
     8821 ggcaataaaa ccgtctggcg ataatat
//
LOCUS       DAZGCS010000157         8817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGCS010000157 DAZGCS010000000
VERSION     DAZGCS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.159"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..707
                     /locus_tag="ACHK01_004476"
     CDS             <1..707
                     /locus_tag="ACHK01_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO8099299.1"
                     /translation="LKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRS
                     YIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQAL
                     RSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIFILFSSADD
                     GPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALFP
                     IFTASWRATRIKNIGI"
     gene            930..1427
                     /gene="ubiC"
                     /locus_tag="ACHK01_004477"
     CDS             930..1427
                     /gene="ubiC"
                     /locus_tag="ACHK01_004477"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO8099300.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1440..2312
                     /gene="ubiA"
                     /locus_tag="ACHK01_004478"
     CDS             1440..2312
                     /gene="ubiA"
                     /locus_tag="ACHK01_004478"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO8099301.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2467..4890)
                     /gene="plsB"
                     /locus_tag="ACHK01_004479"
     CDS             complement(2467..4890)
                     /gene="plsB"
                     /locus_tag="ACHK01_004479"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO8099302.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5061..5429
                     /gene="dgkA"
                     /locus_tag="ACHK01_004480"
     CDS             5061..5429
                     /gene="dgkA"
                     /locus_tag="ACHK01_004480"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO8099303.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5539..6147
                     /gene="lexA"
                     /locus_tag="ACHK01_004481"
     CDS             5539..6147
                     /gene="lexA"
                     /locus_tag="ACHK01_004481"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO8099304.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6220..7545
                     /gene="dinF"
                     /locus_tag="ACHK01_004482"
     CDS             6220..7545
                     /gene="dinF"
                     /locus_tag="ACHK01_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO8099305.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7661..7870
                     /gene="yjbJ"
                     /locus_tag="ACHK01_004483"
     CDS             7661..7870
                     /gene="yjbJ"
                     /locus_tag="ACHK01_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO8099306.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7912..8427)
                     /gene="zur"
                     /locus_tag="ACHK01_004484"
     CDS             complement(7912..8427)
                     /gene="zur"
                     /locus_tag="ACHK01_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO8099307.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8745..>8817
                     /gene="yjbL"
                     /locus_tag="ACHK01_004485"
     CDS             8745..>8817
                     /gene="yjbL"
                     /locus_tag="ACHK01_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO8099308.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNE"
BASE COUNT         2077 a         2090 c         2349 g         2301 t
ORIGIN      
        1 tattgaaatc cgttctggca gtaaaactgg ttatgcaatt aaaaattctt aatattgtta
       61 ataaaaactt tattgagaat atgaagaaaa catttagcca ttgtccttat ataaaagatc
      121 caattatacg cagttatatc cattctggtg aagataacaa gttcgatgat tttatgcgtc
      181 aacatcgatt cagcaaggtg gatttcgata cccaacagat gatccatttt attaacaggt
      241 ttaatatgaa taaagggctg attgataaaa ataacaattt ttttatccaa cttatcgatc
      301 aggccttacg atcaacggat gatatgatca aagcaaatgc ctggtatctt tataaagagt
      361 ggattcgtag tgatgatgtt tcacctctat ttatagaaat tgaagataat ttaagaacct
      421 ttaacacgaa tgaattaaca cgaaaggata atatctttat cctgttctcc tctgccgatg
      481 atgggccagt tatggtggta agctcccagc gcttacatga tatgttgaat cctacaaaag
      541 ataccaattg gaattccacg tgtatctata aatccagaca taagatgttg cctattaatc
      601 ttactcagga aacacttttt agctccaaat ctcatggtaa atatgcgctt ttcccaattt
      661 ttactgcgag ttggcgagct actcgtataa agaatatagg tatttaagta aaggaaaaca
      721 tcaccgttcc tggcatcatg ggcggtgatg cccctacggt tgccctcgcc agcacgggca
      781 tcggcaaagc gtaaggttca acagcggttt accacttcat gcgattgttg cgtttttgtt
      841 gcgcattaga tcacttaatt tgctttacat ctcccgtaaa cacttttctg cgatacaatg
      901 cctttacgtt atgtaacgga gagttcggca tgtcacaccc cgcgttaacg caactgcgtg
      961 cgctgcgcta ttttaaagag atccctgccc tggatccgca actgctcgac tggctgttgc
     1021 tggaggattc catgacaaaa cgttttgaac agcagggaaa aacggtaagc gtgacgatga
     1081 tccgcgaagg gtttgtcgag cagaatgaaa tccccgaaga actgccgctg ctgccgaaag
     1141 agtctcgtta ctggttacgt gaaattttgt tatgtgccga tggtgaaccg tggcttgccg
     1201 gtcgtaccgt cgttcctgtg tcaacgttaa gcgggccgga gctggcgtta caaaaattgg
     1261 gtaaaacgcc gttaggacgc tatctgttca catcatcgac attaacccgg gactttattg
     1321 agataggccg tgatgccgga ctgtgggggc gacgttcccg cctgcgatta agcggtaaac
     1381 cgctgttgct aacagaactg tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata
     1441 tggagtggag tctgacgcag aataagctgc tggcgtttca tcgcttaatg cgtacggata
     1501 agccaattgg cgcgttactg ctgctctggc caacattatg ggcgttgtgg gtggcgacac
     1561 cgggcgttcc tcagctctgg atcctggcgg tatttgtcgc gggtgtctgg ctgatgcgcg
     1621 ctgctggatg tgtggtgaat gattatgctg accgaaagtt tgatggtcat gttaagcgca
     1681 cggcgaaccg accacttccc agcggcgcgg taacagagaa agaggcgcgc gcactgtttg
     1741 tagtgctggt actgatttcg tttttactgg tactgacgct gaatacgatg accattctgt
     1801 tgtcgattgc cgctctggcg ttggcgtggg tgtacccgtt tatgaagcgg tatacccatc
     1861 tcccgcaagt ggtgctgggc gcggcgtttg gctggtcgat tccaatggct tttgccgctg
     1921 tgagtgagtc ggtgccattg agttgctggt taatgttcct cgccaatatt ctctgggcgg
     1981 tggcttacga cacgcagtat gcgatggttg accgcgatga tgatgtgaag attggcatta
     2041 aatccacggc aatcctgttc ggccaatacg ataaattgat tattggtatt ttgcagattg
     2101 gcgtactggc actgatggcg atcatcggtg agttaaatgg cttaggctgg ggatattact
     2161 ggtcaattgt ggtggctggc gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg
     2221 agcgtgaagc ctgctttaaa gcatttatga ataataacta tgttggtctg gtactatttt
     2281 tagggctggc aatgagttac tggcatttct gatgatgtaa aaaagccgga tgatcatccg
     2341 gctttcttct gggttgcctg atgcgcggcg cttctcaggc ctacacaaca catcgcaatt
     2401 tattgaattt gcagattatg gaaggccgga taaggcgttt tcgccgcatc cggcaattct
     2461 ctctgattac ccttcgccct gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat
     2521 caactccgcc agcaactgat aaaccttcat cgtttctgcc ggttcggcgt cgccgctatc
     2581 gctgatatac ccttcatcac gcagcgtcag caccagagaa ctgaacaccg ccttgtcgaa
     2641 gaactccggc gcgttgatgc cgtgcagcac ggagagacgt tgcgcgacgg tgcggctctc
     2701 tttctccagc gtaccgcggt tgatcgacgg gttggcactc aacaaccaga aggtgatggc
     2761 ataacgttgc agcgtttcgc gcgcgcctgc ggccagcagc tgtagggtgc gagaatgcgc
     2821 cgggttgata tgcaactcat catcttgcag ggtaatcagc ccctgacgtt gcatctcatt
     2881 tgccagcgca tcaataacgt ccggcaactc gtcgcgatcc cagcgcagga acagctccgc
     2941 tttcagcatt gggtaaagca cattgacgtg ctccatcaat acgtcgcggg agatgtggcg
     3001 atgctgggtg acaattgccg ccatcagcga aggcagcacc aacatatgcg caatgttgtt
     3061 gcgatagtag gtcatcagca ccgcttgctc gcgtggcaga atgatgatgt cgccgatagt
     3121 gtctttctcg acttcaaact tgttcatttg cagcgcgtga tcgataagct cgctggcgct
     3181 ggctgaagga acggtagagt ccgtggagta aggtacgttg cgcatcagat ccaggtagca
     3241 gttgagttgc tcggttaact gctcgcgggt gagtgagcgc tgacgtgatg ccagtagcgc
     3301 agtacagcac aggttcatgg cgtttgccgc gcctgcgttg ttaatgcgta ccatcagatc
     3361 ggcagcaata ttattgaccg tcggcgttaa ccaggccgga cgcaccgctt cgatgggatc
     3421 gatagattca cgccagtctg gtacgtgctg gttaaggtag gtcatcaacg gcattggttc
     3481 accgaagttg acgtaaccct gaccgagatt acgcagcttg cttaaaccgc gcagcatctg
     3541 cggcaggctc tctttctctt tcgtcgcgcc gcgcagttct ttggcgtaag tacccacttc
     3601 catgacgtgc tcataaccga tatagatcgg aatcagcgta atcggacgcg tgccgccacg
     3661 cagcatcgcc tgaatggtca tcgacagcgt accggttttc ggatccagca aacgccccgt
     3721 gcgggaacga ccgccttcca cgaagtactc gacggaataa ccacggctga acagttcgcc
     3781 gagatactca cggaaaacgg tggaataaag tttattgcct ttaaaagtac ggcgaataaa
     3841 gaacgccccc agacggcgga aaatcggccc ggccggccag aagttcaggt tgatcccggc
     3901 ggcgatatgc ggcggcacca gcccctggtg gtacagcacg taagaaagta gcaggtagtc
     3961 catgtgactg cggtggcaag gcacatacac cagctcatga ccgtcgtggg ccagctggcg
     4021 aacgcgctcg gcgttatgga cgttgatgcc ctgataaagt cggttccagg tgaagcccag
     4081 aatgcggtca gtcaggcgga tcatctcgta agagaaattc gccgcaatct cttccatcag
     4141 cgcaatcgcg ttctgctgcg ctttttcatg ggagattttt ttgctgcgcg cttcatcttc
     4201 taccgctttg gcaatggcgc gggaggcgag cagcttatta aacagatcct gacgagcagg
     4261 cagacgtggg cccacagcag ccagacgttg acgggcaaag tgcatacgcg ccacgcgcgc
     4321 cagtttctgt gcgatagttt tatccgtgcc gtgttcatcc gccatacggc gcagcgaaac
     4381 tgacggcgag aaacgcacaa aactgtcgcg accgagccac agtacggcga aaaatttctg
     4441 tacgccgtta agcatacgca gcggcgggtt tacttcgcct ttttcacgcc ccggcgcgcg
     4501 accaaacatc accgatactg gcaccatctg cacatccaga tttgggttgc tacggtgcaa
     4561 atcgagatag tcgtggaaca gcttaataga ctcttctttc ggcgtgtaat aggtgaatac
     4621 acgcggcccg ccgtgaatga acacatagcg cggcagtagc gtgccgtcga tttccagcgg
     4681 ctctaacggg tcaggcaagt catgagccag acactgggcg cgtaacgtca gcaaatctgc
     4741 tttcgagttg tacggtaaaa cgtacataat tggacgagaa gtatccagcc ccagttccgg
     4801 ggcaggatct gccggaatag acttgctttt taccaggatg cttaatggta aattcagtaa
     4861 tttgtagtaa attcgtggcc agccggacat aaacgatgta aagcctctgg ttaataatgc
     4921 aaatgcgcgg caaggatagc agaaagtcat gggaaattct gtggtatccg ctcatgtttc
     4981 gcgcggcgtt acgcaaaccc gaatcatcgg atttaacggt acactgatat tgacgctcat
     5041 aatgtaaaaa ggttctttca atggccaata ataccactgg attcacccga attatcaaag
     5101 ctgctggcta ttcctggaaa ggtttacgcg ctgcatggat caacgaagcg gcattccgtc
     5161 aggaaggcgt agcggtattg ttggcggtgg tcatcgcctg ctggctggat gtggacgcaa
     5221 ttacccgcgt gctgcttatc agctccgtga tgctggtgat gattgtggaa attctcaata
     5281 gcgccatcga agcagtggtt gaccgaattg gctctgaata ccatgagctt tccggacgcg
     5341 ctaaagatat gggatccgcc gcggtgctga ttgccattat cgtcgccgtg attacctggt
     5401 gcattctgtt atggtcgcat tttggataac ccttccagat ttcgataaat ctctggttta
     5461 ttgtgcagtt tatggttcca aaatcgcctt ttgctgtata tactcacagc ataactgtat
     5521 atacacccag ggggcggaat gaaagcgtta acggccaggc aacaagaggt gtttgatctc
     5581 atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt
     5641 ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc acgcaaaggc
     5701 gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga agaggaagaa
     5761 gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccgc ttctggcgca acagcatatt
     5821 gaaggtcatt atcaggtcga tccttccttg ttcaagccga atgctgattt cctgctgcgc
     5881 gtcagcggga tgtcgatgaa agatatcggc attatggatg gtgacttgct ggcagtgcat
     5941 aaaactcagg atgtacgtaa cggtcaggtc gttgtcgcac gtattgatga cgaagttacc
     6001 gttaagcgcc tgaaaaaaca gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt
     6061 aaaccaattg tcgttgacct tcgtcagcag agcttcacca ttgaagggct ggcggttggg
     6121 gttattcgca acggcgactg gctgtaacat atctctgaga ccgcgatgcc gccaggcgtc
     6181 gcggtttgtt tttcatctct cttcatcagg cttgtctgca tggcattcct cacttcatct
     6241 gataaagcac tctggcatct cgccttaccc atgattttct ccaatatcac cgttccgttg
     6301 ctgggactgg tcgatacggc ggtaattggt catcttgata gcccggttta tttgggcggc
     6361 gtggcggttg gtgcaacggc gaccagcttt ctctttatgc tgttgctgtt tttacgcatg
     6421 agcaccaccg ggctgactgc gcaggcttat ggtgccaaaa atcctcaggc attagcccgt
     6481 gcgctggtgc aaccgttgct gttggcgttg ggggctgggg cgttaattgc gctgctgcgt
     6541 acaccgatta tcgatctggc gctgcatatt gttggcggta gtgaagcggt cctggaacag
     6601 gcgcggcgct ttcttgaaat ccgctggtta agcgctccgg cgtcgctggc gaatctggta
     6661 ttactcggtt ggttactcgg cgtgcaatat gcccgtgcgc cagtaatttt gttagtggtc
     6721 ggcaatatcc tcaacattgt gctggatgtc tggctggtga tggggctgca tatgaacgtg
     6781 cagggcgcgg cgctggcgac ggttattgcg gaatatgcaa cattgctgat tggtctgcta
     6841 atggtgcgta aaatcctcaa actacgcgga atttccggcg aaatgctgaa aactgcctgg
     6901 cgaggaaact tccgtcgctt gctggcgctt aaccgcgata tcatgctgcg ttcgctgttg
     6961 ttgcaactct gtttcggcgc gatcaccgta cttggtgcgc gactggggag tgacattgtc
     7021 gctgttaacg cggttctgat gacgctactc acctttaccg cctatgcgct tgatggtttt
     7081 gcctacgcgg ttgaagcgca ctccggtcag gcatacggtg cgcgcgacgg tagccagttg
     7141 ctggatgtat ggcgggcagc gtgccgccag tcggggattg tagcgttact gttttcggtg
     7201 gtttatttgc tggcagggga acacatcatt gcgttgctga cgtcgttaac ccagattcag
     7261 cagctggctg accgctatct tatctggcag gtgattttgc cgttggttgg cgtctggtgt
     7321 tatctgctgg acggcatgtt tataggcgca acgcgtgccg ccgaaatgcg taacagtatg
     7381 gcggtggccg ccgcaggttt tgcgctgacg ctccttacgc tgccgtggct gggtaatcat
     7441 ggtttgtggc tggcattaac cgtctttctg gcgttgcgcg ggctttctct ggcggctatc
     7501 tggcggcgtc actggcgcaa cggtacctgg tttgccgcaa cgtgacggtt aaaaattctg
     7561 aataaattat cctaagccaa attgctgact acacttaatc tcacgttcag aagaaaagtg
     7621 aacgtactct cattcacaac ctaacgatga ggtcttgatt atgaataaag atgaagccgg
     7681 cggtaactgg aaacagttta aaggtaaagt gaaagagcaa tggggcaaac tgaccgatga
     7741 tgatatgacg atcattgaag gtaaacgtga tcaactggtc ggtaaaatcc aggaacgtta
     7801 tggttatcag aaagatcagg cagaaaaaga ggtcgtggat tgggaaaccc gcaatgaata
     7861 tcgctggtaa ttaatccctc ctgcccgacg tgtacaagga tgtacgccct cttaacgcgg
     7921 tttctttttc acctgcacag agtgatcatg ctggcactgt tcaggatgac gacacgcttc
     7981 cacttccacg catgccgcac agagcccatg tgcttcaatc acattatgcc gcagggcaaa
     8041 ccccattttt gccgccagcg tatgcataat gtcttccacg ccttctgcac actcttcttt
     8101 caccgcgccg cagcgatcgc atataaacat ggctgacgta tgcgtgggct ggtcgaacag
     8161 atgacagagc acataactgt tggtggattc caccttatgc acaaaacctt gctcaagcag
     8221 aaaatccagc gcgcgataaa ccgttggcgg cttggcttgc ggttcagctt cgcgcagtaa
     8281 atcaagcaga tcataagcgc tgatagcgcc atcctgcaga ctcatcaggc gcaacacttc
     8341 caggcgctgt ggggtcaggc gcacattacg ctgcgcgcag attttttcag cctgcgctaa
     8401 taactcctgc gttgtggtct tttccatcgg gcacctcaaa gtgggggtta agaatgtcct
     8461 tactttacca tgttccagga aaaacaccga gaacccttat tgttgccgta atgttgattt
     8521 tctgttttgt aggtaaggtg ttgtgttgcc ttgtcgtacc attatcaaca cgataataat
     8581 taataattca tttttaaata aattgcttca agttaactcg ctatttattt aactgggttt
     8641 aaacgcatac ctgtagttca gaaattatga gcgatttttg atagtgacca gatggcaaaa
     8701 gaattataag gaggacacgg tacgacaaaa aaggtagatg aatcatgttg aagataatac
     8761 ccggtgcaac tggatacttt aataaaacac tcaattcaaa tcaattcgat aatgaag
//
LOCUS       DAZGCS010000158         8650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGCS010000158 DAZGCS010000000
VERSION     DAZGCS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.160"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHK01_004486"
     CDS             complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHK01_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO8099309.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            662..880
                     /locus_tag="ACHK01_004487"
     CDS             662..880
                     /locus_tag="ACHK01_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO8099310.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            888..1829
                     /gene="ribF"
                     /locus_tag="ACHK01_004488"
     CDS             888..1829
                     /gene="ribF"
                     /locus_tag="ACHK01_004488"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO8099311.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLALGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1872..4688
                     /gene="ileS"
                     /locus_tag="ACHK01_004489"
     CDS             1872..4688
                     /gene="ileS"
                     /locus_tag="ACHK01_004489"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO8099312.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4688..5182
                     /gene="lspA"
                     /locus_tag="ACHK01_004490"
     CDS             4688..5182
                     /gene="lspA"
                     /locus_tag="ACHK01_004490"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO8099313.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHK01_004491"
     CDS             5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHK01_004491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8099314.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5721..6671
                     /gene="ispH"
                     /locus_tag="ACHK01_004492"
                     /gene_synonym="lytB"
     CDS             5721..6671
                     /gene="ispH"
                     /locus_tag="ACHK01_004492"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO8099315.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6737..7651
                     /gene="rihC"
                     /locus_tag="ACHK01_004493"
     CDS             6737..7651
                     /gene="rihC"
                     /locus_tag="ACHK01_004493"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO8099316.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7818..8639
                     /gene="dapB"
                     /locus_tag="ACHK01_004494"
     CDS             7818..8639
                     /gene="dapB"
                     /locus_tag="ACHK01_004494"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO8099317.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESDLFDMRDVLDLNSL"
BASE COUNT         1971 a         2158 c         2465 g         2056 t
ORIGIN      
        1 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
       61 ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      121 ttctacgatt cttaagccac gaagagttca gaaagtgtaa cggcatgtct gcaaaagcca
      181 gcatcttttg actatctggc aaccggtagt gtgttctctc acgcatcata aaagcagcag
      241 gcataaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt ggcgattaag
      301 ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg tttttgtgga
      361 tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat gctttctgtg
      421 cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa gtacgcatca
      481 tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg cgcttcttag
      541 ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac aattcaaagg
      601 ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt
      661 aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg cggggtgtga
      721 atacagcttt tccgcgataa aaattgcagc aggcggtcac tttcttcccg tgatttgcgc
      781 catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt taaccttgac
      841 cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg aagctgatac
      901 gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact attggtaatt
      961 tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca
     1021 agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa ctgtttgcta
     1081 ccgataaagc cccggcacgg ctgacccggc tgcgggaaaa actgcgttac cttgcagagt
     1141 gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg tttcgcggcg ttaaccgcgc
     1201 aaaatttcat cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt gccttaggtg
     1261 atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag aaagctggca
     1321 tggaatacgg cttcgatatc accagtacgc aaactttttg cgaaggtggc gtgcgtatca
     1381 gcagcaccgc tgtgcgtcag gcgctggcgg atgacaatct ggctctggca gaaagtttac
     1441 tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta gggcgcacta
     1501 taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg aaaggggttt
     1561 atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca aacattggaa
     1621 cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta gatgttgcaa
     1681 tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc
     1741 gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg
     1801 aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg aaacacggaa
     1861 ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca gggttcccga
     1921 tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact gatgatgatc
     1981 tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg catgatggcc
     2041 ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt ctgaaagaca
     2101 ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct ggctgggact
     2161 gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg ggtgagaaat
     2221 tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag gttgacggtc
     2281 aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg tacctgacca
     2341 tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc ggcaacggtc
     2401 acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct gcgctggcgg
     2461 aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct tttcaggcgg
     2521 tcgatcagga tgcactgaaa gcaaaatttg ccgtaagcaa cgttaacggc ccaatctcgc
     2581 tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac
     2641 ctgatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc
     2701 tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc ggcacggtaa
     2761 aaggtgcgga gcttgagttg ctgcgcttta cccatccgtt tatgggcttc gacgttccgg
     2821 caatcctcgg cgatcacgtt accctggatg ccggtaccgg tgccgttcac accgcgcctg
     2881 gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc gctaacccgg
     2941 ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc gtgaacgtct
     3001 tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg
     3061 agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg
     3121 cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag tcactgaaag
     3181 agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag tcgatggttg
     3241 ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg atgtcactgt
     3301 tcgtgcacaa agacacggaa gagctgcatc cgcgtacact cgaactaatg gaagaagtgg
     3361 caaaacgcgt tgaagttgac ggcatccagg cgtggtggga tcttgatgcg aaagagatcc
     3421 tcggcgatga agctgatcag tacgtgaaag tgccggacac attggatgta tggtttgact
     3481 ccggatctac ccactcttct gttgttgacg tgcgtccgga atttgccggt cacgcagcgg
     3541 acatgtatct ggaaggttct gaccagcacc gtggttggtt catgtcttcc ctaatgatct
     3601 ccaccgcgat gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc tttaccgtgg
     3661 atggtcaggg tcgcaagatg tctaaatcca tcggcaatac cgtttcgccg caggatgtga
     3721 tgaacaaact gggcgcggat attctgcgac tgtgggtggc atcaaccgac tacaccggtg
     3781 aaatggccgt ttctgacgag atcctgaaac gtgctgccga tagctatcgt cgtatccgta
     3841 acaccgcgcg cttcctgctg gcaaacctga acggttttga tccagcaaaa gatatggtga
     3901 aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg
     3961 aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag cgtctgatgc
     4021 gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac cgtcagtaca
     4081 ccgccaaagc ggacagtgtg gcgcgtcgta gctgccagac tgcgctgtat cacatcgcag
     4141 aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa gtgtggggct
     4201 acctgccggg cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa ggcctgtttg
     4261 gcctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg aaagtgcgtg
     4321 gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt ggctcgctgg
     4381 aagcggcagt aaccttgtat gcagaaccgg aactgtcggc gaaactgacc gcgctgggcg
     4441 atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat aacgacgcac
     4501 ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg agtaaagccg
     4561 aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag gtggcggaac
     4621 acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt
     4681 ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc tggtggtagt
     4741 cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg ctctggggga
     4801 tacggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt
     4861 tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg
     4921 tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga agctaaacaa
     4981 tatcgcttat gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca
     5041 cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa
     5101 ccttgccgat actgccattt gtgtcggtgc ggcactgatt gtgctggaag gttttttgcc
     5161 ttctaaagcg aaaaaacaat aataaaccct gccggataag gcctacagat tgctgcgaaa
     5221 tcgtaggccg catccggcaa aaatcctcaa aatataagag caaacctgca tgtctgaatc
     5281 tgtacagagc aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac
     5341 cgccgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg atgcttctct
     5401 ttctgaaggg ctggagcaac acctgttggg gctgaaagtg ggcgataaaa ccaccttctc
     5461 gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg
     5521 ccgtgaattt atggatgcag gcgagccaga aattggcgca atcatgcttt ttaccgcaat
     5581 ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga
     5641 tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga
     5701 tccggcactg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg
     5761 gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat
     5821 atgtccgtca cgaagtggta cataaccgct atgtggtcga tagcttgcgt gagcgtgggg
     5881 ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt ttctccgcac
     5941 acggtgtttc tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtatttgacg
     6001 cgacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagt cgccgtggcg
     6061 aagaatctat tctcatcggt cacgccgggc acccggaagt ggaagggaca atgggccagt
     6121 acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc ggacgatgtg tggaaactga
     6181 cggtcaaaaa cgaagagaag ctctccttta tgacccagac cacgctgtcg gtggatgaca
     6241 cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag
     6301 atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg gcagaacagg
     6361 cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggagc
     6421 tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagac atccaggaag
     6481 agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc
     6541 tggtgcagaa tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc attccgctgg
     6601 aaggccgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg
     6661 aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat
     6721 tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga
     6781 tgccgtcgcc attgccgccg cgatttttgc ccccgaactc gacctgcaac tgatgaccac
     6841 cgtcgcgggt aatgtctcgg ttgagaaaac tacccgtaat gccttgcaac tgctgcattt
     6901 ctggaatgcg gagattccgc tcgcccaagg ggccgctgtg ccgctggtac gcgcaccgcg
     6961 tgatgcggca tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa
     7021 ccgaaagccg ctcgggatac cggcgtttct ggcgattcgg gatgccctga tgcgtgcacc
     7081 agagcctgtt accctggtgg ccatcggccc gttaaccaat attgcgctgt tactttcaca
     7141 atgcccggaa tgcaagccgt atattcgccg tctggtgatc atgggtggtt ctgccggacg
     7201 cggcaactgt acgccaaacg ccgagtttaa tatcgctgcc gatccagaag cggctgcctg
     7261 tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg gatgtcacca atcaggcaat
     7321 attaacccct gactatctcg ctactttgcc ggagttgaat cgcaccggaa aaatgcttca
     7381 tgcactgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa tgcacgatct
     7441 ctgcgccatc gcctggctgg tgcgcccgga cctgttcact ctcaaaccct gttttgtggc
     7501 agtggaaact cagggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct
     7561 gggcaagcca gccaatgtac aggtggcatt ggatctggat gtgaaaggct tccagcagtg
     7621 ggtggctgag gtgctggctc tggcgtcgta acctgtcaca tgttattggc atgcagtcat
     7681 tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca
     7741 gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt
     7801 aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg
     7861 ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg
     7921 cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg
     7981 cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat gattttgatg
     8041 tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc
     8101 atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcgattc
     8161 gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg
     8221 tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa
     8281 ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg
     8341 gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg
     8401 aaggccacac cggtgaacgt gtgcctggca ccattggttt tgccaccgtg cgtgcaggtg
     8461 acatcgttgg tgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcactc
     8521 ataaggcgtc cagccgcatg acatttgcta acggcgcggt aagatcggct ttgtggttga
     8581 gtggtaagga aagtgatctt tttgatatgc gagatgtgct tgatctcaat agtttgtaac
     8641 cacaaaatat
//
LOCUS       DAZGCS010000159         8641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGCS010000159 DAZGCS010000000
VERSION     DAZGCS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.161"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..940)
                     /gene="glxR"
                     /locus_tag="ACHK01_004495"
     CDS             complement(62..940)
                     /gene="glxR"
                     /locus_tag="ACHK01_004495"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8099318.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKALFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1040..1816)
                     /gene="hyi"
                     /locus_tag="ACHK01_004496"
     CDS             complement(1040..1816)
                     /gene="hyi"
                     /locus_tag="ACHK01_004496"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO8099319.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPKTTTEAGLRWMDPYR"
     gene            complement(1829..3610)
                     /gene="gcl"
                     /locus_tag="ACHK01_004497"
     CDS             complement(1829..3610)
                     /gene="gcl"
                     /locus_tag="ACHK01_004497"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO8099320.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3700..4515)
                     /gene="allR"
                     /locus_tag="ACHK01_004498"
     CDS             complement(3700..4515)
                     /gene="allR"
                     /locus_tag="ACHK01_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO8099321.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4593..5075)
                     /gene="allA"
                     /locus_tag="ACHK01_004499"
     CDS             complement(4593..5075)
                     /gene="allA"
                     /locus_tag="ACHK01_004499"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO8099322.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5305..6231
                     /gene="allS"
                     /locus_tag="ACHK01_004500"
     CDS             5305..6231
                     /gene="allS"
                     /locus_tag="ACHK01_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO8099323.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPFGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6300..7394
                     /gene="mnmH"
                     /locus_tag="ACHK01_004501"
     CDS             6300..7394
                     /gene="mnmH"
                     /locus_tag="ACHK01_004501"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO8099324.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7510..7875
                     /gene="ylbG"
                     /locus_tag="ACHK01_004502"
                     /pseudo
     CDS             7510..7875
                     /gene="ylbG"
                     /locus_tag="ACHK01_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8396..>8641)
                     /locus_tag="ACHK01_004503"
     CDS             complement(8396..>8641)
                     /locus_tag="ACHK01_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099325.1"
                     /translation="SYFSLTNDDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYG
                     KIPGQGDGVNLFFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         2202 a         2261 c         2024 g         2154 t
ORIGIN      
        1 ttcttttttt aatattgctg gccgattcat tttctgatat cggccatttt tattgcgggt
       61 atcaggccag tttatggtta gccattaatt ccagcgcctg cactaacgca gagtgatcca
      121 actggctgcc accgtttgcc gcgcaggtat taaataactc ctggcaggtc gcagtgtttg
      181 gcaggttcag cgctagcgct ttcgcacttt gcagtgccag gttgagatct ttctggtgca
      241 gcgcaatttt gaaacctgga ttaaaagtgc gtttaatcat gcgctcaccg tgaacttcca
      301 gaatacgtga ggaagcaaag ccgcccatca gcgcctggcg cacacgtacc gggtccgcac
      361 cggcttttga agcaaatagc agggcttcag aaaccgcttc aatattgagc gccacaataa
      421 tctgatttgc cactttgcag gtttgaccat cgccgttacc gcccacgagg gtgatatttt
      481 taccgagcag ttcaaacagc gctttaacac gttcaaatac cgcttcatca ccgccaacca
      541 taatcgacaa cgtcccttca cgcgcaccga tttcaccgcc ggagactggc gcatcgagat
      601 aatcgccgcc cagttcattc acctgacgag cgaaacgctt agtttcaatc ggggaaatgg
      661 agctcatatc aacaatggtt ttgcccttca gcgaggcttt ggtacaacca ttttcaccga
      721 acagaacttc ttcaacctgt ggtgtatctg gcaccataat aaaaatgatg tccgatgctt
      781 ccgttacctg gcgcgcagtt tcaacactga cggcacccag tgacagtaat tcatcagcaa
      841 ccggaccaat ggttgtgaca tgcagttgat gaccggcacg cgccagatta atggccatcg
      901 gtgtacccat aatgcctaag ccaataaatc ccagtttcat aattaacctc ttttaaattt
      961 cgcttttcct gaattcagac aacacgatcc cctgccaact tagcggccta aaaaagcatt
     1021 gcctgaacag cgttacgttt taacggtacg gatccatcca gcgtaaaccg gcttccgtgg
     1081 tggttttggg tgtatattca cacccaaccc aaccgttgta gtcagaattt tcgattacct
     1141 taaagagata atcataatta atttctccgg tccccggttc gccgcgatgc ggattatcag
     1201 caatttgcag gtgaccaatt ttatcggccc actgagtcat ggtgttggtt aattcacctt
     1261 ccatccgctg catgtgataa atgtcatact gaattttcaa attgcagcaa ccaacatcat
     1321 caatcaattt cagcgcctgc cgagttccgg tgagatggaa accaggaata tcaaaatggt
     1381 taataggttc aatcagtaat aaaatatctt ctttcatcag catattcgcg gcataacgca
     1441 ggttttctac aagcgttgcg tgaatctgtt cactgctgaa accagccggc gttttaccga
     1501 ccagacagtt aattttttta ttacccagcg cacgggcata acgaatcgct gctgctacgc
     1561 catcccgaaa ctcttcttca cggccaggaa tacaggcaat accgcgctcc cccgccgccc
     1621 agtcacctgc cggtaaattg tgcagcgtat gttcgagttt attactcgcc agcacctgtt
     1681 ttaattcttc aatgtcgtag tcataaggaa acataaattc aacgccgcga aaaccacact
     1741 gcgcagcttt ctcaaaacgg gcgagaaaat catattcacc aaataacatc gataaattag
     1801 cagagaaacg taacattatt tatctccctt attcatagtg catgaagcag gtttcagtcg
     1861 gtgcatccgc tgcgttatcg gcgatatctt caaattccat gacgttatcc agttcgctgc
     1921 ccatcgaaat attggtcaca cgctcgagaa taacttccac gactaccggt acccgatatt
     1981 gcgccattaa ggctttcgcc tgttcaaagg ctggcgcaat atcttccggt ttgaagacgc
     2041 gaatagcttt acaacctaaa ccttccgcta cttttacgtg gtcaacaccg tagccattca
     2101 cttcactgga gttgatattc tcgaaagcga gttgcacgca gtagtccata tcaaacgcgc
     2161 gctgcgactg gcgaatcagc cccagataag cgttattgac cagtacatgg atgtacggaa
     2221 tgttgaactg tgcgccaacg gccaattctt caatcaggaa ctggaagtcg aagtcaccgg
     2281 aaatcgccac cactttgcgc tctggatcag cggcacaaac ccccagcgcc gccggaatgg
     2341 tccagcccag cgggcctgcc tgcccacagt tgatccagtg gcggtcttta aagacatgca
     2401 gcatttgcgc agcggcaatt tgcgacagac caatggtggt gacatagcaa acatcacgtc
     2461 caaaagcttt gttcatctct tcatacacac gctgcggttt aaccggaacg ttgtcgaagt
     2521 gtgttttacg caacaaagta cgtttgcgct gctggcactc agcaacccac tctttacggc
     2581 atggcagacg ccctgctttt tgcatttcct gcgccacttc aaccagcagt gtcagcgccg
     2641 ctttagcatc agagacaatc cccagatccg gacacagcac gcggccaatt tgcgtcggct
     2701 caatatcgat atgaacgatt ttgcgccctt cggtgtattt ctctaccgaa ccggtatgac
     2761 ggttagcaaa acggttaccg ataccaaaca ccatgtcgga cgccagcaac gttgcgttac
     2821 cgtaacgatg cgcggtttgc agacccacca tcccggccat cagttcatga tcgtccggga
     2881 tacagcccca gcccatcagc gttgggatca ccggaacgct ggtcagttca gcaaactgtt
     2941 gtaacagtgc agctgcgtca gcattaatta ccccgccccc ggcaacaatc accggacgtt
     3001 tggcctggat taacatttct acagcttttt cgatctgcat acggctggca gcaggtttgt
     3061 agaccggcag cggttcgtac atatcaggat caaactcgat ttccgcaacc tgaacgtcga
     3121 acggtaaatc caccagtacc ggtcccggac gaccagaacg catcaggtga aatgcctgtt
     3181 gcagcacacg aggcaccagc gccgcttcac gtactgtaac cgccattttg ctgaccggtt
     3241 tagcaattgc ttcaatatct acggcctgaa aatcttcttt atgcagacgg gcgcgcggtg
     3301 cctggccggt aatgcacaga ataggaatgg aatcagcaga agcggaatag agcgcggtga
     3361 tcatgtccgt gcccgcagga ccggaagtcc ccagacatac gccgatattc cctgccgttg
     3421 cgcgggtata accttccgcc atgtgcgaag caccttccac atgacgcgcc agaatgtgac
     3481 gaataccgcc gtgcttacgc atcgctgagt agaacggatt gattgcagct cccggaacac
     3541 cgaaggcggt ggtgatacct tctttctcca gcacatacat tgccgcgtca acggctctca
     3601 tttttgccat tttattccta cctctattta ttggaaaatt tttccaactt tcaaattttg
     3661 tcaaaaaaac ccgctccgca gagcgggatg cgacagacat tatggatgtg ctttcagtcc
     3721 caacgccgtg ctgatatcgc gggcggtgtc tctgaccagc tcaccctgac tgacaaaacg
     3781 atcttctgtc agtcttgatg aaggcccgga gatggagata gcggcaacaa cgctaccgac
     3841 atcatcgtaa attgctgaag ctatgcaatt cagacctaca acatgctctt ctttatctac
     3901 ggtatagccc agttcacgcg cttgttccag gtccttcagc aaggtgggca tatccacaag
     3961 cgtagttggc gtaaactgct gcaaaccggt ttgcagaatg atgctcatca actcctcttc
     4021 cgccagcgga taaagcaacg ccttgcccgc accggaagca tgcagcggca gacgactgcc
     4081 cagtggcgca cacatcctga ccattgattt acactctaac tgaccaatta atactgcttc
     4141 attgccgtta cggatcgcga cattgaccgt ttcgccggaa agtaacatca ggcggcgcat
     4201 aaacggcccg gcgacggaga ggacatcgcg gttatggatg tacgccgcac cgacgttaaa
     4261 gacacctaat cctatatgcc accagcctaa ttgactgtcc tgatagacaa aatccgctgc
     4321 ttgtaaaacc ttcagcaagc gaaaggtcgt ggagagcggc aaatccagat tgagagaaat
     4381 atcgctaacc gacgaacttc ccccactttt ttccagatat tgcagaatcg caattccccg
     4441 ctctaacgcc tgtgcgccct tctgtgccac aggctccgcc tgtcctggcc tgccgcgccg
     4501 tctaacttcc gtcataccta cgttaactcc gacctacaag tgagcataaa caatgaacaa
     4561 ttagtttatc ttatgcaagt cggttgcagg cgtcacgcaa aacagagttc ctgttcagga
     4621 atactttcaa catcacagtt gtcactgccg ccgcgatcga tggtcagaaa atcggtgacg
     4681 cgctgccagg cgaaaagtgg gtgatgccag acgttacgat ggtaattcac tccctgttcg
     4741 ccgttggtga taaacgcccg cagcgttgac aggtctggtt tgtcgtcgcc taacgccacg
     4801 accaccacaa acacttcacc tttcatcggg ataaaggcct gagtgcccag cggatgacgt
     4861 tcgagttcgt aaatggtcag cggcagattc gccggttgcg cgcggttaat gctgataagc
     4921 gtacggtctt gctcaagaat ctcaaccagc gccaaatcgt ggtaacgctc caccaggcca
     4981 ttgttaatat ggaaaaaatc ccgttgctga gtttcgatta cgtcgccata agcactaaag
     5041 gcttcctgac ttaacggtaa tacctgaagt ttcatctttt tgtccttgta acataattcc
     5101 gttcaaccat cgccgaccgg tcagcttggt ttcaataaca ggcaatctaa aaccttaaga
     5161 gaacaacatc aatacaaaag ttggaatatt tttccaaaac tagatttagt ccacaaattg
     5221 aacagggtta tcatgtaaaa atactgataa atcagaggat aagaaaatca cgcaacttgc
     5281 ggatttaaaa ggggatacct gaagatgttc gatccagaaa ccttgcggac tttcattgcg
     5341 gttgctgaaa caggaagttt ttcaaaagcg gcagaacgat tatgtaaaac cacggcgacg
     5401 atcagttatc gcattaaact tctggaagag aataccggag tagcgctgtt tttccgcacg
     5461 actcgcagcg tgacgttgac agcggctggc gagcatctac tttcccaggc cagagactgg
     5521 ctgagctggc tggaaagtat gccaagcgag ctgcaacagg tgaatgatgg cgtggaacgc
     5581 caggtgaata ttgtcatcaa caacctgctc taccaccccc aggccgtcgc ccagttgctg
     5641 gcgtggctga atgaacgtta cccctttacc cagtttcaca tctcccgaca aatctatatg
     5701 ggcgtctggg attcgctatt atacgaaggt ttttcactgg ctatcggcgt cacgggaacc
     5761 gaggcgctgg caaatacctt tagtcttgat cccttcggat cggtgcaatg gcgctttgtc
     5821 atggcggcgg atcatccgct ggcgaacgtt gaagagccgc taacagaagc gcagttgcgg
     5881 cgctttccgg cggtcaatat tgaagacagc gcccgcacct taaccaaacg cgtcgcctgg
     5941 cgattgcctg ggcaaaaaga gattattgtt cctgatatgg aaacgaaaat cgccgcccat
     6001 cttgcgggcg ttggcattgg ttttttgcca aaatcgcttt gccagtcaat gatcgataat
     6061 caacaactag ttagccgggt aatcccaacg atgcgccctc cttcgccatt gagtctggca
     6121 tggcgcaaat ttggcagcgg caaagcggta gaagatattg tgaccttgtt tacccagcgc
     6181 aggccggaaa tcagcggatt tttagaaatt ttcggcaacc cacgcagtta aaaatcaagt
     6241 ttccttgcgc gttggcgcgt aaaatagcgc gcaagctgat ttgaggttca cttttaacta
     6301 tgcaagagag acacacggaa caggactatc gtgccctgct gattgctgat acgcccatta
     6361 ttgatgttcg cgcccctatc gagtttgagc aaggcgcaat gcccgccgct atcaatctgc
     6421 cgttaatgaa taacgatgaa cgcgccgccg ttggcacctg ctataaacag caaggctcag
     6481 acgcagcgct ggcgctgggg cataaactgg tggcgggtga aattcgtcag cagcgcatgg
     6541 acgcctggcg ggcagcgtgc ctgcaaaatc cgcaaggtat tctctgctgc gcccgtggcg
     6601 gtcagcgctc acatattgtg caaagctggt tgtatgcagc ggggattgat tatccgctgg
     6661 tggaaggcgg ttataaggca ctgcgccaga ccgcgattca ggcgactatt gaactggcac
     6721 aaaaaccgat agtgctgatt ggcggttgta ctggcagcgg taaaacgctg ttagtgcagc
     6781 aacagccgaa cggtgttgat ctggaagggc tggcgcgcca tcgcggttcg gcgtttggtc
     6841 gcacgttaca accacaactt agccaggcga gttttgaaaa cctgctggct gccgaaatgc
     6901 tgaaaaccga cgcccgtcag gatttgcgcc tgtgggtgct ggaagacgaa agccggatga
     6961 tcggttcgaa tcacctgccg gaatgcctgc gcgagcgaat gactcaggcg gcgattgcgg
     7021 tggtagaaga tccgtttgag atccgtcttg agcgcctgaa cgaagagtat ttcttgcgta
     7081 tgcatcatga ttttacccac gcttatggcg acgaacaggg ctggcaggaa tattgcgaat
     7141 acctgcatca cggactttcg gcgattaagc gtcggctggg gttacagcgc tataacgaac
     7201 tcgctgccca gctggatacg gcgctgacaa cgcaactcac caccggcagc accgacggtc
     7261 atctggcctg gctggtgccg ttacttaaag aatattacga cccaatgtat cgctatcagc
     7321 ttgagaaaaa agcggaaaaa gttgtctttc gcggtgagtg ggcggaagtg gcggaatggg
     7381 ttaaggcgcg gtaatgaggg acgtatttgt attagtcgcg acttgatctg acacatggcc
     7441 ttgaaaggtt gagagttacc ggttttgata tgagtgtcga atccttatcc aaaacaagag
     7501 gtaactctca tgcttcatac tgccaatccc gtcatcaaac acaaagccgg tttgctcaat
     7561 ctggctgaag aactcagcaa cgtatcaaaa gcctgtaaaa tcatgggcgt ttcgcgtgat
     7621 acgttttatc gttaccgcga actggtcgct gaaggcggtg tggatgcgca gattaatcgt
     7681 agtcgccgcg ctcctaacct taagaaccgt accgatgagg caactgaaca ggctgttgtt
     7741 gattacgccg tcgctttccc gacacatggt cagcaccggg ccagcaacga gctgcgtaaa
     7801 cagggggttt ttatctccga cagtggtgtc cgttctgtct ggttactcca taaccttgag
     7861 aatctcaaaa gacgatactg aacaaatttt atcaggtgac gttccgtaaa aagttgtatg
     7921 gagttctcga tgcagtcggc gcgactatca gtacccccgc tgaaccctgg actatcgggc
     7981 cgccgtactg gggcatgtca ccctggcgtg ccgctggttt tccgctaatg gtttatttcc
     8041 tctttctttc ccggcaagaa tttattttaa tccggaagga ttatttattt tcagataggg
     8101 actcacatta aacacgtaac attttaatta atgcatggct gaatatgtat tacacaatgt
     8161 cagatatcgt aaaagaccag tgcaattcta tgtaaactct ctgactgttc attttatttg
     8221 ttgtttcagg gtcggaactc accctcaata aacaactgaa tacgagtaga tattcatcgt
     8281 ctgagctata tggctttaca caatagccga catgtctttt ctcagtcagg aattttactt
     8341 ttcctcacta cgaagttgac gacttcgata tgggatagac tcttaattca agcaattact
     8401 ctgcacaagc atcaggcaat ccagctccaa tatttgttat aaaataatga gtaacattaa
     8461 tttcaccaac aaaaaaaagg tttacaccat ccccttgccc tggtatctta ccatatttac
     8521 accacacttt ttttgtatct ggatcattct ttgatacgat tttcgccact tgttccctgg
     8581 ccctcactgt caatgttttc ttaaacctac ccccatcatc atttgttagg gaaaaataag
     8641 a
//
LOCUS       DAZGCS010000160         8531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGCS010000160 DAZGCS010000000
VERSION     DAZGCS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.162"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(422..1183)
                     /gene="envY"
                     /locus_tag="ACHK01_004504"
     CDS             complement(422..1183)
                     /gene="envY"
                     /locus_tag="ACHK01_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO8099326.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1366..2256)
                     /locus_tag="ACHK01_004505"
     CDS             complement(1366..2256)
                     /locus_tag="ACHK01_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO8099327.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2257..5229)
                     /gene="nfrA"
                     /locus_tag="ACHK01_004506"
     CDS             complement(2257..5229)
                     /gene="nfrA"
                     /locus_tag="ACHK01_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO8099328.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5216..7453)
                     /gene="nrfB"
                     /locus_tag="ACHK01_004507"
     CDS             complement(5216..7453)
                     /gene="nrfB"
                     /locus_tag="ACHK01_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO8099329.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7722..>8531)
                     /locus_tag="ACHK01_004508"
     CDS             complement(7722..>8531)
                     /locus_tag="ACHK01_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO8099330.1"
                     /translation="GKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEIT
                     AIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFP
                     LKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1984 a         2167 c         2181 g         2199 t
ORIGIN      
        1 ttttgtttag tattgaatga ttatcacaat gcgctatctg tttttggttt aattatctgt
       61 tattgtttta tgttgcggtt ttactgtgtg gttttttatg cttttgtggt gtttttatct
      121 atttaagcgc catgccttta aaggcatata agcgaaaata gcatgaggtt tatcctcaat
      181 tgctatgttt tttagtacaa aaaagaggga caaaactgag acacataagg cctcacaatg
      241 gcttgcaagg ctttacatgt tttgaggtag tgggacgtgt gagcgcagag atggcgcggt
      301 aatttgttga cttaaaatgt cgttctagga acttctaagt cgtgggccgc aggttcgaat
      361 cctgcagggc gcgccatttc ttcttcattt atgcccgtct tatccgtttc cgctttgccc
      421 ttcaccacat cactttttgt cgctgtttgg cgagataatt caacggtgtc aggccgtaaa
      481 acgccttaaa aacagagata aagtatgacg tgctgctata gccacataat tgcgccacct
      541 gagtgatatt tttgttatcc atcaataaca tctgtacggc gtaacgcata cgacactctg
      601 tgacaatctg gctatagctg gtattttcgt tttttaattt ctttttgagc aggctggggc
      661 ttaaacatag cgaactggcg acaattcgca gattccagta atgctgaata tcgctttgaa
      721 taatgcggca gacgctgtcg cggacgctgc tgcgtaagat atacatcagt agggcaataa
      781 accgcgattg ctcaagaaag ttagacaata cggtaaaaag caatacgcgc gtcaactccg
      841 cttcgcaggg tttgctgggt tgctgcttgc tgtggttggc ggctagccgg aacacttccg
      901 gtgtacggct atgacaagca ataaccgggg ttgccagccg cgaccagggc tttacacagg
      961 ttaagttctt atttaaaaac aacagataat cgttgatgat gttacggttg atgtgggtga
     1021 ttaatacgtg atttaacgtt gagagttcaa tgacgttatt gttgcacgcg aaggcggcca
     1081 ggtagttttt cggaagggta aacgtagcat ggttattgac gcttacctct acctcttttt
     1141 cggtcaggat caccacgcaa ggttcactgc tgctcaattg catttcgcac tcctcagata
     1201 tcagaaactc cgctcaaagg atctatgctt cctgcatgag tgatcggccc gttcgccgat
     1261 aacgatcttc tttctttagc acgcttttta gcaattaatc ttgatggaat tctgacgaga
     1321 gcgaaagagg taagccaggt cgtacccgac ttacctggag gagatttaat actcgagaat
     1381 gccgtgcgcg acgggcaaat agcgcagaga gaaatagaga gtgtctttac cgcaagagga
     1441 acgtttcgct aacagcgagg cgatctctgc atccggtttc ggtttcgctg taaaggtttt
     1501 gcctttgccg gcgacaaaca gttcataaac aatgccgctg gcaggggcgg gactttggca
     1561 atcggcgctg gcctgtagat aacgttcacg ctgttcagcg gtaagtttat ccacgccgct
     1621 gccatcctgt acccagacat taacgccggt tgctttaact tgttccagca gttggcgata
     1681 gccatcgggc gacatgtttc cggcgaaaaa actactgata taaaccggtt ttgctgaaac
     1741 atcgctaatc agccgctgcg cgttgtttaa ccatgttagc aaaggctgac gggcagcttc
     1801 gctgcgccag ttcaggtcgt caatttccgc gctgatgtac cagccatccg gcgttacgcc
     1861 aggcgcggcg ctccataatc tggcttgctg gagatcggca gccagcaggc gattaagata
     1921 gctttccagc gctgcggacg actgtttctg gtgcataaaa aattccggat cggcgttcag
     1981 cccgacaatc agcttcaggc cagcctgttg cgcagctgcg gcacgcttaa acaataacgc
     2041 gcgctgttct ggctgggtga atgcatcgcc gtaacgggtc cattgcaaaa ccagggtatc
     2101 gaagccttgc aaacgtaact gactcatcag cccctgccac tgggtatcgg taacctgact
     2161 atctcggttt tgtggttgcc agataatacc tttcatcgca aaggaaaaag ggctgaccaa
     2221 aagcagtgtc agcaatacga aaatgaactt acgcatttac cagtgcactc caatggtgag
     2281 aaacgcgttg ttgcgctctc cgttacgttg attaatcgcc ttaaaggtat gttgatactc
     2341 gacgccgaga ctgactttgt gcggccaggc gtcgtagtgc gtctcgccgg tccagatatt
     2401 ccagcggacc ccgactccgc caagctgcgc gccctgagtg cctttatcac gatagccgtt
     2461 gtcctgaacg tgagcgtaag gctcaatagt ctgtccgtta gctaccttct gatgccagct
     2521 gacgcgataa tctgccgtcc acgcctgaat atcctggcgg atatattgcg ccgcatcgag
     2581 gtacaggttt tgggcaaacc agcctgaacc gttcgggtgc cattcgtcgc tgtatttgcc
     2641 gccattaaag aatgaggcgc tggcgcgcag catggtatcg gatgcgccat tttggccgtt
     2701 cagcggcaac tgctgttcga cggcgaggaa aaagatctga tcgcgcagcg gcttccagcg
     2761 cagaccggtg ccggacatcg gatttttcac cggcatcatc accccgtttt ctccggtatc
     2821 ggcaaagacg cggctataaa ctgagagcag gtcgccttcc agcagcatat tgcgtccgat
     2881 gcggtactcg gcttccagtt gcccgtagct acgatagctt ttccctggcg ctgcgccgcc
     2941 gacattattg ttagcggtgc tcattgcccc ggaacgcaag ccgatagaag aatcgaaact
     3001 gaacgtccag cggcgaccga cctcctcatg caaacggcgg aaattgaagc gttgctgatt
     3061 ttgctctggg gtcagtgggg ttatcagcgc ctgattatca atgtcatcaa tcaccagccg
     3121 ggcgtagtgc tgcgtcgcag gcatgtcatc cagacgctgg ttcacgtagg ccagttgtcg
     3181 gatcagtgcc ggatcgtccg gaagcccttt atgcgcctgt tcgagcattt cccgcgactg
     3241 tgcgatatca ccgctatccc acaaggcgta accaagcgct gcctggatgt tgctattatt
     3301 cggttccagt tccagcgcgg cgcgcaaatc actcaccgcc gccgggacat tatgacgttg
     3361 gcgataaatt gtcgcccgcg caacgtaagc gttggcagaa ggggcaatat tgattgagcg
     3421 cgtgagatcg ctcagtgcga gttccggctg accaggaatg taacgttgcg catgcagcca
     3481 ccagtagagg gcattgtttc ccagtccacg ttgttctgcc tgttgtagcc agcgatcgcg
     3541 agctgcacca tttcctgccg cctgggcggt attggcagca gcaagcagat cctcattgct
     3601 catgtcgtga agactgattt tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg
     3661 atacgcctga taggctaccg cacgatgttg ccaggcgttc ggttgtcgtt gttcggcctg
     3721 aagccatgca tacagcgcca caccgggtag cgtgtcccga taacactttg ccagacggtt
     3781 ccaggcggcg gcatcgtagg aaggcgacat atcgcccagc aagcgaacta ttgccgggca
     3841 attatctgca atacccggca actgactttg ccactgacgt tgctccgcca gcggtaaggg
     3901 tttcgataaa atcgccacct tcgccggcgt tgccaggtaa ggatgacttt ccagcagaga
     3961 cgccagtcgc gccattaaag tctggctgac acgcgcatcg ccctggaaag gatagcgttg
     4021 cagcaataaa tcggcagctt cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg
     4081 atccaggcgg gtaagatttg ccggttcttg ctgatacagc aatcgtgcca gacgcagaga
     4141 ttcagccttg ttacgggtcg ccacgctgac agcataacgc tcctcaagca tttcattggc
     4201 ggggagggtg gcgagcagtt tttgcgctgc gtcgtactga ccttctttta acagcaccgg
     4261 tagcgtcgcg ccaacaacat actggcggtt atcggcaaac tgcaccgtat aattcgccaa
     4321 tgcctgaacg gggttggcgc tgtatttaga taacagatag agccaacttt tctcttgtgc
     4381 gtccgtggta aacagcggct tattttcatt gagataatgc tggaggcgtg ctttttcgcc
     4441 acgataagcc agcgcggtcg cgtaagtaat atatgactga ggatcggtga agatcccctg
     4501 tgattgcagt gccaggatcc gatcgtccag ctgcccggca agaagcacgt caaaccactg
     4561 acggcgttct gccgcgctta atgtgttctg ctggcgtgct tcattgtata gcgtatctgc
     4621 ctgggaccat tgtttcaggt agattgcccg ttgcagcaga tcggttcgca gcgtttttcc
     4681 ttctggcgat gcagcaaacg tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc
     4741 cagccgcagg gcattctgcc cgacttcact gcgacaacgc agggtcggcg cagcgtcgca
     4801 cgctttttgc tgggcaagca gttcttcaac agtcgtaacg cttttcactt caaccggaat
     4861 agccgccaga ctgcgctcaa gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag
     4921 caacagccgc gcccggtcat catgaccaaa atggcgatag gcttccgcaa ggtataaagt
     4981 cagcggaata ttatccggca cctgctggtg tatatattca aattcgcgga tggcggttgc
     5041 ttcgtcgtta tttttctgtg ccttcagcgc cttatcgaga cggggataaa taacaaaatg
     5101 gcgataatcg ctcagcccca gctcttctgc gctggtgccg atattgtctg cgagtgcgct
     5161 ggtactcaat aaagacgtca gcagtaaacc agaccatccg atgacgcgat taaggttatt
     5221 ctccttcatt ttcggactcc agttgcgcaa cctgttctgt gtttaaacct gctttgagta
     5281 atagtgattg catcgaaact tgtaattcgc gttgaattgt caggacgcga tccaacgttt
     5341 cctggctgat aacgccttcg gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat
     5401 ggcgcaataa caacacgtta attgctgaac gattaatatg accgagcgtg gtcagtattt
     5461 cggcgaacag gaactgatgc ggcacatatt gccgccagat ttcaccggcc tgctgttccg
     5521 tgagccactg atgctgaacc gcattgtaca acattgcccg cggatcgtga ccgcgtcggc
     5581 gtgcatacca gtgacgtaac cccgtgacaa tttgtccccg cagaacaatg acgtaacgca
     5641 ctttgcgtcc gactttacgc gtcagggccg ccagcgaaac cgggtcaata ccatcttcac
     5701 tgccgacaat taactcatca ttttccagac gcagcggcag tactgcataa tgcagcgcta
     5761 cggaggccgg catttcggca atcagcgagg acgggatctg ccaggcatcg atggattccc
     5821 acgccacgcc gttttgctct gccagcgcct gtgccagctg ctcggcgcta atcagcccct
     5881 gcatcagcat tgaaccgccc aagcgtagac cttcgacgcg attacgcagt gctgtatcga
     5941 gttgttcttc agtgatgacc tgattttcca gcagaatttg gcctaacggg cgcaacgagc
     6001 gggtatcgcc agtcacgctg gggaagtcat gcgttgtttt atcccacgcg acgcgacgtg
     6061 gatcgccgtg ttgaagtacc tgttttagcg cgcgccagtt ggccatgaag ttaatcaggt
     6121 tgccccagaa aagacgcagg acagataaca gcccctgcgt cagaccgtag tagccagtga
     6181 cgaaaatcac ccgctgcacg atgcggttaa ccatcaagcc aaagtttagc cacagcaggg
     6241 tcattaacca tgcgctgccg ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac
     6301 tttcatacgc cagcaacagc aaaagctgga gcatcaccag catcgcgagg aagctgacaa
     6361 agttactgat tgcccctttg cggtcgcgcc agagaaagta gttcagcgtc aggctggagg
     6421 tccatttgtg ggttttaaag ccctggaaaa caatgccgat gatccagcga gatttttgtc
     6481 gaactgcagt cgaaaaggta tcggggaaat attcgcgcac gcagatcatg tttgacgtcc
     6541 gtgcgtgctg taaaaattta cgctgctcgc gttctttggc ttcgtccacc accggaaaac
     6601 ggacaaaaat ttccgtcata cctttctctt tcaggcggaa accaatgtcg taatcttcag
     6661 tcagactctg cacgtcgaaa gcaataccgt caccgtcagc taacagagcg gtcacggcgc
     6721 ggcggctgaa acaggtgccg acgcctgcgc tgggcacttg tccggcgagg gcttcacgca
     6781 ccggaacatc tttgccatgc agctctgaaa actcatcaat gtaagtcatg ctggtgaagt
     6841 gcgtccattc gcgttcgaac ggatacaccg ggatctgaat cagatcttta cgctcgacca
     6901 gatagttgaa cagacgcaat tccatcggtg aaatcacatc ttcggcgtca tgcagaataa
     6961 aaccagcaaa agcgaaattg gcgctacgtt caaactgggt gatggcgtcc agcacgttgt
     7021 tcagacagtc ggctttgctg gtgggaccag gacgcgcgca gactacctta tgcacgttcg
     7081 gaaagcgagc gcacacttcg tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc
     7141 caacaaagat atgatagttt tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc
     7201 cgatgacgcc cgtttcattc cacgccggaa ccataatcgc taacggtttt tcatctggtt
     7261 tatacagttc gcggtaactc attcgcgggt agcggcgata aacactcaac ttgcgtttaa
     7321 tgcggcgtac ccagtagacg acatcaataa aaaaatcgtc cagtccgctg atgaacatga
     7381 tgaccgctaa cgttatcgcg attactttta agccgtagag ccaggttgca aaaacatcaa
     7441 gaagccagtc cacacaaaaa ccttacatta acgctggtta tgtttagggt ggcgtatatt
     7501 aaggtttttt atgaattgtg acagcttttt accattaata ggtatgacta ttgcggcacg
     7561 ttatttttac actggttata aaagttgccg tttgcgtaga atgtagacaa aagcgtggtg
     7621 tcaaggaggg gaggttaacg tcatagtgaa gaggagtggt ggcaccgatc aggtcatcat
     7681 gtagtggaag tgcctggagg aggtgagcag gcagggcaag attacgaaag cccgctcccc
     7741 gcaaggactg acgccagata gtttctgtcc atggctgctt ttcgcatctt acgtcttaac
     7801 cctgccttga ataccttatc attcgtcaaa atattaatag cgatgtgccg tatccctgaa
     7861 aataattctg ctgcatttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc
     7921 agacgccagt gcagcttatt ctccacgtgc cagtggtttc ggattgctgt ggcgaacttc
     7981 tctgcggtta aatcagcaga actgatataa tatctgaccg tcatttcggg ctctttcttt
     8041 tgttctgcta ttattgaccg aaaggagact gccacgcata atttcttcag acctttccat
     8101 tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa caatatgaag acggatttct
     8161 tctctgccgt gactcttttc actgattgcg taactgtcat gctctggatt atttaattct
     8221 ttcagtggaa atttttcctc aaaggcttta tttagccgcc cctggtttcc ttttaccgcg
     8281 aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg gcaacccatc
     8341 gcatcagttg tgatgatttt tcctttaata tccagcatgt taagaagttc tgggatagct
     8401 gtaatctcat tagatttctc atccgtcttg atctgtccga tgaccagact gtgcattgtt
     8461 gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata agagtgccgg
     8521 agcgtttttc c
//
LOCUS       DAZGCS010000161         8379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGCS010000161 DAZGCS010000000
VERSION     DAZGCS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.164"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(106..312)
                     /locus_tag="ACHK01_004509"
     CDS             complement(106..312)
                     /locus_tag="ACHK01_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO8099331.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            625..714
                     /gene="kdpF"
                     /locus_tag="ACHK01_004510"
     CDS             625..714
                     /gene="kdpF"
                     /locus_tag="ACHK01_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO8099332.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            714..2387
                     /gene="kdpA"
                     /locus_tag="ACHK01_004511"
     CDS             714..2387
                     /gene="kdpA"
                     /locus_tag="ACHK01_004511"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO8099333.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2410..4458
                     /gene="kdpB"
                     /locus_tag="ACHK01_004512"
     CDS             2410..4458
                     /gene="kdpB"
                     /locus_tag="ACHK01_004512"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO8099334.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4467..5039
                     /gene="kdpC"
                     /locus_tag="ACHK01_004513"
     CDS             4467..5039
                     /gene="kdpC"
                     /locus_tag="ACHK01_004513"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO8099335.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5032..7716
                     /gene="kdpD"
                     /locus_tag="ACHK01_004514"
     CDS             5032..7716
                     /gene="kdpD"
                     /locus_tag="ACHK01_004514"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO8099336.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSYDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7713..>8379
                     /gene="kdpE"
                     /locus_tag="ACHK01_004515"
     CDS             7713..>8379
                     /gene="kdpE"
                     /locus_tag="ACHK01_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO8099337.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYR"
BASE COUNT         1739 a         2179 c         2449 g         2012 t
ORIGIN      
        1 ttcgattggt ataacgatgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
       61 ccagcgtttc cgctggcggt tttcaatgta ttcaaaactc agcaatcaat aaaaatcacc
      121 agttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      181 atgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      241 aacgcccgct gcaaccgcat aagtacggcg taaaaagata agccatgcag gatattctct
      301 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatggca ccggaaaaat
      361 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      421 cacttatttt tgcttattga tggtttattt acattcatcc tgtaattaag ttacacaaaa
      481 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      541 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctaccct tccggtatca
      601 cttttaggcc actggaggtg cactgtgagt gcaggcgtga taaccggcgt attgctggtg
      661 tttttattac tgggttatct ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
      721 cgcaagggtt cttactgatc gccacgtttt tactggtgtt aatggtgctg gcgcgtcctt
      781 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
      841 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
      901 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
      961 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1021 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttacagc ggtgaaacca
     1081 cgttgagcta tttcagccag atggctggct taacggtgca aaactttctt tctgccgcca
     1141 gcgggattgc ggtgattttt gccctcatcc gtgcgtttac ccgccagagc atgagcacac
     1201 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctcgtc cctgtggcgt
     1261 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttcag ccttatcagg
     1321 ctgtgaatac cgttgaagga gcgcaacagc tgttacccat ggggcctgta gcttctcagg
     1381 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1441 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1501 caacggcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1561 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggtgatg tgggcagaag
     1621 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1681 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggctt
     1741 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggtggc atggtgccga
     1801 tgtggctgat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     1861 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     1921 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg
     1981 ttaccccgac gctggtgctg atgggcgcgg cgctggcaat gatgaccgac gccggacgta
     2041 gcgccatgct caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcatccg
     2101 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactccccg ttctggaact
     2161 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2221 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2281 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2341 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagtga
     2401 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2461 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2521 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2581 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2641 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     2701 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     2761 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     2821 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     2881 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     2941 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3001 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3061 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3121 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3181 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3241 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3301 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3361 caggcgtcgg agtttatccc tgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3421 caactggctt cgctggctga tgaaacgccg gaaggccgca gtattgtgat cctcgccaag
     3481 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3541 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3601 gtcgatgcca ttcgccgcca tgttgaggct aacggcggtc acttccctac cgatgttgat
     3661 caaaaagtcg atcaggttgc gcgtcagggg gccacgccgc tggtggtggt ggaaggttcc
     3721 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     3781 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     3841 gccgccgcca ttgctgcgga agcgggcgtc gatgattttc tcgccgaagc gacaccggag
     3901 gccaagctgg cattgattcg tcagtatcag gcggaaggcc gtttggtggc gatgaccggc
     3961 gacggcacca acgatgctcc agcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4021 ggtactcagg cggcgaaaga agcgggcaat atggtcgatc tcgactctaa cccgaccaag
     4081 ttgatcgagg tggtacatat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4141 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4201 acgtatccgc agttaaatgc gctgaacatc atgcgccttc attcgcccga ctccgccatt
     4261 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4321 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4381 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4441 gtttgcggtc tggtgtgagg tttaccatgc gtggcttacg tccggcatta tcgacattta
     4501 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgtg ctggggcaat
     4561 ggtggtttcc ctggcaggcc aacggttcgt tgattcgtga aggtgatacg gtgcggggtt
     4621 cggcattaat cgggcagaat tttaccggca acggctattt tcagggtcgc ccgtcggcaa
     4681 cggcagaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     4741 ctgagctgga taaacaaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     4801 ccagtacgaa cgttccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     4861 tcaccccgca agcggcggtc tggcaaatcc cacgcgtggc gaaagcgcgt aatctcagcg
     4921 ttgaacagct cacgcaactg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     4981 gccagccggt cgtcaacatt gttgaactca atctggcgct ggataaactt gatgaataac
     5041 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5101 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5161 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccat
     5221 gggcggaaag ataccgccgc catgcttgaa gggctggctg ttctgccgcc caaacgccag
     5281 gcgtaccgtg ggcggcatat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg
     5341 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa
     5401 cgctggcagg atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac
     5461 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5521 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tagtgctggt ggacttgccc
     5581 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5641 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgtga actggcactg
     5701 cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct ggcgggggca tcctggtgaa
     5761 gagaaagtgt ggcacacgcg cgacgcgatc cttttatgca tcgggcataa caccggcagt
     5821 gagaaactgg tccgcgcggc ggcgcggttg gcctcgcgcc tgggtagcgt ctggcacgcg
     5881 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaaa aaaaacgtcg ggcaattctc
     5941 agcgccttac gtctggcgca ggagctgggc gcggagacgg ctacactttc tgatccagcg
     6001 gaagaaaaag cagtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6061 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6121 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6181 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6241 gttgccgccg cgttatgcgc cgttatcact ttaattgcca tgcagtggct gatggcgttt
     6301 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6361 ggacgctggc cttcggtggt tgctaccgtc attaatgtgg tgagttacga tctctttttt
     6421 atcgccccac gcggcacgct cgctgtctct gatgtgcaat atctgctgac cttcgcggtg
     6481 atgttaaccg tcgggctggt gatcgggaac cttactgcgg gtgtgcgtta tcaggcgcgg
     6541 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6601 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6661 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     6721 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     6781 cctgcgggtg cgggcaccga cacgttaccc ggcgtgccgt accagatttt gccgctaaaa
     6841 agcggcgaga aaacctacgg actggtggtg gtggaaccgg ggaatctgcg tcagttgatg
     6901 atcccggagc agcagcgcct gctagagacg tttacgctgt tagtcgccaa tgcgtttgag
     6961 cggctgacgc tgaccgccag tgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7021 cgcaacgccc tgctggcggc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7081 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcgcccca cgcccgccag
     7141 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7201 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7261 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7321 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7381 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7441 cacgttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7501 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcagtaccg
     7561 ggggtagggc ttggactggc aatttgtcgg gcgatagtgg atgtacacgg gggcactatt
     7621 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7681 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     7741 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctatg
     7801 aggccgaaac gctgcaacgc ggcttgctgg aagcggctac ccgtaagcca gatttgatta
     7861 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     7921 ggagcgcggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aaaatcgccg
     7981 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8041 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8101 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8161 tgcatctcac accgattgag ttccgcctgc tggcggtgct gctgaataat gccgggaaag
     8221 tgctcaccca gcgccagtta ctcaatcagg tgtgggggcc aaacgcggtc gaacacagtc
     8281 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat cccgctcgcc
     8341 cccgccattt cattactgaa accggtattg ggtatcggt
//
LOCUS       DAZGCS010000162         8336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGCS010000162 DAZGCS010000000
VERSION     DAZGCS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.165"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(115..191)
                     /locus_tag="ACHK01_004516"
     tRNA            complement(115..191)
                     /locus_tag="ACHK01_004516"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Asp,seq:gtc)
     gene            complement(324..1055)
                     /gene="dnaQ"
                     /locus_tag="ACHK01_004517"
     CDS             complement(324..1055)
                     /gene="dnaQ"
                     /locus_tag="ACHK01_004517"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO8099338.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1120..1587
                     /gene="rnhA"
                     /locus_tag="ACHK01_004518"
     CDS             1120..1587
                     /gene="rnhA"
                     /locus_tag="ACHK01_004518"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO8099339.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1584..2306)
                     /gene="yafS"
                     /locus_tag="ACHK01_004519"
     CDS             complement(1584..2306)
                     /gene="yafS"
                     /locus_tag="ACHK01_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8099340.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2340..3095
                     /gene="gloB"
                     /locus_tag="ACHK01_004520"
     CDS             2340..3095
                     /gene="gloB"
                     /locus_tag="ACHK01_004520"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO8099341.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3167..4525
                     /gene="mltD"
                     /locus_tag="ACHK01_004521"
     CDS             3167..4525
                     /gene="mltD"
                     /locus_tag="ACHK01_004521"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO8099342.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSSVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4573..5343)
                     /locus_tag="ACHK01_004522"
     CDS             complement(4573..5343)
                     /locus_tag="ACHK01_004522"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8099343.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTLEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5421..6221)
                     /gene="yafD"
                     /locus_tag="ACHK01_004523"
     CDS             complement(5421..6221)
                     /gene="yafD"
                     /locus_tag="ACHK01_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8099344.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6462..7376
                     /gene="yafC"
                     /locus_tag="ACHK01_004524"
     CDS             6462..7376
                     /gene="yafC"
                     /locus_tag="ACHK01_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO8099345.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7373..8176)
                     /gene="dkgB"
                     /locus_tag="ACHK01_004525"
     CDS             complement(7373..8176)
                     /gene="dkgB"
                     /locus_tag="ACHK01_004525"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO8099346.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2232 a         2000 c         2019 g         2085 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg aatttggcgg
      121 aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccgactga
      181 actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat tgcctcaccc
      241 ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc caagccgcta
      301 ttttcagcgc cttttacagg tatttatgcc cgccagaggc agcttccgcc cttcttctcc
      361 accagatcaa gacgggcttc atgagctgca agctcttcat ctgtcgcaaa aacaacgcgt
      421 aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg ctgctgtgtc
      481 tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag ataaacttcc
      541 gcaaggatct gtgcatcgag taatgccccg tgtagcgttc gtttactgtt atctatttcg
      601 tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt cctcgccacc
      661 gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg cttaagcaac
      721 gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac caactccgcg
      781 ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt atcgagcaaa
      841 aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg atcgggtttg
      901 agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac ggcaccaatc
      961 tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt ggtttcggta
     1021 tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt cagacttatc
     1081 gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca ggtagaaatt
     1141 ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg gttacggcgc tattttacgc
     1201 tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa caaccgtatg
     1261 gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga agtcattttg
     1321 agtaccgaca gccagtatgt ccgccagggg atcactcagt ggatccatag ctggaaaaaa
     1381 cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg gcaacgtctt
     1441 gatgctgcat tggggcagca tcaaatcaaa tgggaatggg ttaaaggcca tgccggacac
     1501 ccggaaaacg aacgctgtga tgaactggcc cgtgctgcgg caatgaatcc cacactggaa
     1561 gatacaggct atcaagtcga agtttaagcc tgtggtttac gatattgccg ggtggctcca
     1621 accgcctggc gaattcgtgg cttgttttta ctctgtttca tcggatttag cgttaaagga
     1681 atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat atgtgcattc
     1741 aatagttttc caccgtgttt gttccacggg agaacgtgga aacggctggc gtgcagcact
     1801 tcaaaattca acaaagagag ccagtccagt tgccgcatca gagtaaacat ccggctgtta
     1861 tagggcgagg ttttgcgcaa taccggcaca agtttgcgta atcccattaa actgatggga
     1921 ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggc ttcacgcaat
     1981 aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac atcaacggat
     2041 ttatcggcaa aaggaagatg aagtgggtcc gcctgtacct ggacgggcat tccttgcgca
     2101 gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc gcttaaattg
     2161 ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag ctggcgctcc
     2221 agcgccttgc gataaagctc tccccagggc aaatcgcccc agcaatcagg agccacgaca
     2281 gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactaag aggtaatcta
     2341 tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg aatgatgaag
     2401 caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac gccattaccg
     2461 ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat cacgttggcg
     2521 gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca caagagacac
     2581 aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt ttggggcatg
     2641 aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac ttcagtaaac
     2701 cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg tttgaaggga
     2761 cagcatcaca aatgtatcaa tcacttaata agttaagtgc gttacctgac gatacattgg
     2821 tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt attcttccgc
     2881 acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca aaaaatcaaa
     2941 taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta agaacagaag
     3001 atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct gaagaacgtt
     3061 ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc atcggctccg
     3121 ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga aggcaaaagc
     3181 gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg ttcaacagca
     3241 cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa gtcaggcacg
     3301 atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt tcattggcga
     3361 cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac agaaatattt
     3421 acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata tgtactggat
     3481 agccgggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac ccatagtgga
     3541 gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct ggcagatcat
     3601 tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg cgcgtcgcga
     3661 tgttgttgct tcaacaactg ccgcgctgaa catgatgcag cgtctgaaca agatgtttga
     3721 cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg tcatgaaggc
     3781 aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac cgttgccgca
     3841 ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca aaaacagcaa
     3901 gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc gtgtgcacct
     3961 gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg tcagcaagct
     4021 gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtggtc cgcaatacgt
     4081 gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctcgcttcag gcgaaattgc
     4141 tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagcagtg tttacaccgt
     4201 acgctctggc gacacgcttt caagtatcgc ttcacgtctc ggcgtaagca ccaaagattt
     4261 gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt tgacgattgg
     4321 tgcaggcagt agcgcacagc gactggcaaa caacagcgat agcattacgt atcgtgtgcg
     4381 caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca aagatgtgat
     4441 gcgctggaac agcgacactg cgaatctgca accaggcgac aagctgacgt tgtttgtgaa
     4501 aaacaacaac atgccagatt cctgacaaac cagataataa aaaggcaccg attcccccgg
     4561 tgcctttttt atttatgctg ctttatgtgc ttctaccatg atgatatcac tggtgaaaga
     4621 gccatcattc tgcaaggcaa aatacgttct cacctctgtc gatgcgctct gttggtaaat
     4681 gcgaatagcg tctactaacg cttctagcgt acgcattctc gcgacccatg aagaaaattc
     4741 cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat tgattaacct
     4801 caaccactca ccgctggcgt agtttcgtac gtgagaggta tcgcgtaatg cttctaccgt
     4861 ctgtaaccag atgtcgcgca ctggatgacc cggagacatt acgtccatca caatcatcct
     4921 accgccaggt ttcaatatcc tattcacttc tcgcagtgct gcaccaacat catgccagtg
     4981 atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg gcagactttc
     5041 ggcataaccc tggcgggtgg tgatattttt cagttgccgg gcttcggcag cttgtgccac
     5101 gacatccagc atttgggcag ataagtcata cgccaccacc gcgctcacgt tctgcgcagc
     5161 gacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat caggataatc
     5221 agccagacac accgccaggc gctgtaaatc tcgcccggat gcatgtacgg tactggttaa
     5281 atactcactg gcctgcgagc taaactgctt ttctacgtgg tcatggtggg attgtgttgt
     5341 cattgcgctg tccttttgtt gtttgagtat aaaaggacag cgccaaacgt tggtctgccc
     5401 tgtggcagac ctgacatacc ttatttatca ggcttgccgg gactgaattc aacgagtagc
     5461 ggattgtgat cggaagcgcg cgtaaccaat acagaagctt cgctgacgtt cagaccacgg
     5521 tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc gctgatcatc ggtaaaacgc
     5581 acctggcgca gcgacatttc ccgcgcaaag cgatataacg cgttcatcct tctacggctc
     5641 caggcattga aatctcctgc cataatgacc gggccgctgt ggtgagctat ctgatcacca
     5701 ataggaagta actgcttact atagacatcc acgcccagac tgaagttgac ggcgtgtata
     5761 ttaaccacca tcaacaggcg ggtgtcaggt aatggataga ccgtcaccag tgccgacttc
     5821 gccagacgca aaatgggttc tcgttcacgt aacgggcagc aatacactgg atgcgccgcc
     5881 gaaagggtca ttacgccaga aggatgttgt ggcagtacga aagcgggtac ctgatcggcg
     5941 gcaagatagt tagcggtcgc aaactgtact aactctggcg ttgtctgcgc ttcctgcaat
     6001 aacaccagat gtgcatcttt gccgtagttc tttaataccg acaaccattc agcgcgttgc
     6061 tgtttgtata tgttccacac caggatccga atacgctctt cggtacttaa cggttctccg
     6121 ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt
     6181 tgtccggcaa catagcgcat ggcataggtg ttttttcgca cttttgactt tgaaacctct
     6241 attacgtcaa cccttccgca ctcgggaggg ttctactgtt atagggattt tagctcactc
     6301 tttcaacgag caattgtata ttgttatgta agcaagtgct tacaccggtc agaattttgc
     6361 tcattatgga gaacattaaa tcgcatgaca tatcacgcat cctgatggtt taatgacaaa
     6421 taaattcaaa atatctcttc tattttgcaa caggagcaaa aatgaaagcc acgtcggaag
     6481 aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat
     6541 tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa gctggagatg aaacttggcg
     6601 ttagcctgct taatcggacc acgcgacaac ttagcctgac ggaagaaggc gagcgttatt
     6661 ttcgtcgcgt acagtcaatt ttgcaggaga tggcatcggc agaatcagaa attatggaga
     6721 cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg gtgctgcact
     6781 ttctgatgcc gttaattaag cctttccgtg aacgctatcc ggaagtcact ttgtcgctag
     6841 tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg
     6901 gtacgttaac ggattccagc ttacgtgcca ggccgttatt taacagttat cgaaaaatta
     6961 tcgcctcccc cgattatatt tcccgctacg ggaagccaga aacgatcgac gatttaaagc
     7021 aacatatttg cctgggattc actgaacccg cttccctcaa tacctggccg atagcccgta
     7081 gcgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg gaaacactga
     7141 aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg atcgacaaag
     7201 aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca gtggaaatgc
     7261 cattcagtgc agtctattac agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg
     7321 atttccttag cgagcatgta aaaacagctc ccggaggagc tgtcagagag gcttaatccc
     7381 attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg
     7441 cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactt tccaggtttt
     7501 cacgtttagt agaagaagga attactgagt aaccttcccc catagcccac gccagaatca
     7561 cttgtgccgg agtcgcattg tgtttagctg cgatacgagc aataacctca tctttcaggg
     7621 ccttaccata cgccagcgtc atataggaag taatatggat gccgtgctgt ttagcccaga
     7681 caaccacttt acggttttgc agataaggag agagttcaat ctggttagta gcgatgtttt
     7741 cagcgccaac agcagcaatc gttttttcca tcaacgggat cgtgaagttg gaaataccga
     7801 tctcacgtgt cagaccttgt tttttggctt ccagcagcgc ctgcataaac tcttcaacag
     7861 agacttcatc gtttggtgac ggccagtgga ttagcgtcag atcaacataa tcggtacgca
     7921 atttttgcag gctctctttc agacttggga tcaatttgtc tttgctgaga ttttcaatcc
     7981 agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca atcgcctgac
     8041 ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa
     8101 gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagta cctaaaccaa
     8161 atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg aggtgattat
     8221 gggcttcaga ctgttgcgga aaaagagcgt aaaacgcaga ggatttttgc gattctggca
     8281 ataatagaaa tgtagacgaa aaaaaacctc gagcgataac tcgaggcttc ttaata
//
LOCUS       DAZGCS010000163         8274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGCS010000163 DAZGCS010000000
VERSION     DAZGCS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.166"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1512
                     /locus_tag="ACHK01_004526"
                     /pseudo
     CDS             1..1512
                     /locus_tag="ACHK01_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1481..3217)
                     /gene="aceK"
                     /locus_tag="ACHK01_004527"
     CDS             complement(1481..3217)
                     /gene="aceK"
                     /locus_tag="ACHK01_004527"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO8099347.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3498..4802)
                     /gene="aceA"
                     /locus_tag="ACHK01_004528"
     CDS             complement(3498..4802)
                     /gene="aceA"
                     /locus_tag="ACHK01_004528"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO8099348.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4832..6433)
                     /gene="aceB"
                     /locus_tag="ACHK01_004529"
     CDS             complement(4832..6433)
                     /gene="aceB"
                     /locus_tag="ACHK01_004529"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO8099349.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLASCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6702..7631)
                     /gene="metA"
                     /locus_tag="ACHK01_004530"
     CDS             complement(6702..7631)
                     /gene="metA"
                     /locus_tag="ACHK01_004530"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO8099350.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7788..8231
                     /locus_tag="ACHK01_004531"
     CDS             7788..8231
                     /locus_tag="ACHK01_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8099351.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2131 a         2045 c         2043 g         2055 t
ORIGIN      
        1 tgaacaccaa accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa
       61 agagctggcg acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca
      121 tatctttagt aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa
      181 gaaagaaatt acgataatta ataaaaacga aaacacgaca cttactcaaa ccattgcccc
      241 aatatttgaa gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca
      301 agaattaaac ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg
      361 tcaatgttat tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca
      421 atatattaat gatatttatc agaatgttga ttacaaaccc catcaagatg attactcttc
      481 agctgaaaaa tttctcacgc acttcaacaa aaaatgcaaa aaccagactt tggcgttggt
      541 ttccagccgt cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa
      601 agcatatttt gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt
      661 ggataaccat gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca
      721 ttacgtggtt tcggtttatg atcctaatgt aactaacgat aaaataagaa ttatgagcga
      781 aagcaaagag gatattaaac actattctct gatggatttt atgaatgtag attatagcct
      841 cctgaaatgg tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc
      901 gaaagaacag ctattgatgt taaaaggatc tgtggatgaa ataacccctc cattatcacc
      961 agcaacgatg aatttgctaa tggcaattgg tcagaatcac caacttacgc aactgatgat
     1021 tcaactccag aaaatgccag aactacatag aacagaaatg ttgactgcct ataatagtgg
     1081 acatatgaac gttattaata ctatttttaa cgcattaccc actcttttta atacgtttaa
     1141 attcgataaa aaaatatgaa gcccctcctc ctggcaaata attctaatga atatcccggt
     1201 ttgttttcag cgatacagca taaacaacaa aacgttgtag agacggttta tcttgcttta
     1261 tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt ttggcaacac
     1321 aaagcgccac aaaaatactc tgcctttgag ttggcttttg aatttggtca ccgggttatt
     1381 gctgaattaa tccttaatac attaaataag atggctgaaa gctttggctt tacggataac
     1441 cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc atctccccat
     1501 accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca tgcccttcac
     1561 gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg tgcatctctt
     1621 caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct tccgggaaaa
     1681 catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg
     1741 gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg taatcataaa
     1801 aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccaggaaaaa
     1861 tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact
     1921 gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc tcgatataga
     1981 gatggcgaat tacaatctgc tcaccaagat cggtgatttt ttccgctgct tcctggagca
     2041 gtaattccat taatgccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct
     2101 gggtgtccgc cattcggccc acgcgatcgt gctctttcac cagttgatag caggcacgaa
     2161 cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc
     2221 gatcaaagcc cggcagcgta aacaccaaca tcaccattcc acgaataccc ggcgcttcaa
     2281 tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg
     2341 cgtgcttctg gcagccgata gccatataca attcagcagt ggttttacct ggcagaattt
     2401 cccgtagcca ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg
     2461 caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca ataaataact
     2521 cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa cgtgccggaa ggcgtgatca
     2581 gtttgcccac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat
     2641 gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa
     2701 tgtcacggct tttattctgc cagcgcaggc gcagcggcag gtcgctgata acgcgcatca
     2761 gtagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac
     2821 gaaagcggcg ctctggctga gagctaaaga taaaaagccg ctcgggagta agcgagcggt
     2881 ggtcaaataa ccgacagtac acggagttaa aaaagctctc cgcaatctcg aagcgcgggt
     2941 aatccggcaa cagccgcgtg taatgctctt taacgcgcag taaaaatgcc gcgtccgtac
     3001 tttggccgtt agtaatgcag cgcagttgct ccacgaccag accaacgtga tgatcgtaaa
     3061 gatggatacg gtttttcatc gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct
     3121 gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa
     3181 tggtttgagc aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga
     3241 gaattgccgg atgcggcgta aacgccttat ccagcctacg ttcggcaacg gttgtaggca
     3301 tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa
     3361 tgccttatcc ggcctacgtt cggcaacggc tgtaggcatg ataagacgcg caagcgtcgc
     3421 atcaggcatc gtgcaccgat tgccggatgc ggcgtgaacg ccttatccgg cctacagtca
     3481 gcaacggttg ttgttgctta gaactgcgat tcttcagtgg agccggtcag cgcggtgact
     3541 gaagacgtgc cgccctgaat aatagtcgtc actttatcga agtaacctgt acccacttcc
     3601 tgctggtgag atacgaaggt atagccatct ttcgcggcgg caaattccgg ctgctgcact
     3661 ttctcaacgt agtgcttcat accctcgccc tgggcatagg cgtttgccag gtcaaacatg
     3721 ttgaaccaca tgctgtggat acctgccagg gtgatgaact ggaacttgta gcccatatcc
     3781 gacagctgct gctggaagct ggcaatagtt ttgtcgtcga ggtttttctg ccagttgaac
     3841 gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat agcttgtgca
     3901 aagcgacgcg ccagttccag atccggcgtg gaggtttcac accagaccag gtcagcatat
     3961 ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt acggaagaag
     4021 ccttcactgg tacgctcgcc agtaataaat tcactgtcat acgggtcgca atcggaggta
     4081 atcagatccg ccgcatcggc atcggtacga gcaaccagca gggttggaac gcccgtcacg
     4141 tcagctgcaa gacgcgccgc gaccagtttc tgaatagctt cctgagttgg caccaaaact
     4201 ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa gtgaactgcc
     4261 gctgcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac accgccaaaa
     4321 ccggcttccg catcggcaac gatcggcagg aagtaatcga catagcgcgg atcgcccggc
     4381 tcaatgcctg cggaccattg gatctgatcg gcacgacgga aggtgttgtt gatccgctcc
     4441 accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat gctggccgcc
     4501 aggttagcgt ccgccgctac ctgccatccc gacagataga ctgcttcaat acccgctttc
     4561 gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc tgttgatgta gccttttttc
     4621 gactcgccat gcagcagacg ccacattttc gccgcgccca gttgcgccag ggtgcattca
     4681 ggattgactg aaccgcgtaa tttcaccaca tcttccgcgc tgtatgggcg agtaatgcct
     4741 tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt acgggttttc
     4801 atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag cctggcaggg
     4861 tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt gcggcatcgt
     4921 caaaacgccc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact ttcatctctt
     4981 cacccagcat ctggcggaac aaggctttgg tcaccggttt gccattgctc aacgtttttt
     5041 gatgatggat ccactgccag attgaggtac gggaaatttc agccgtcgcc gcatcttcca
     5101 tcaggccata aatcggcacg cagccgttgc cggagatcca tgcttcgatg tactgcactg
     5161 ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc accatcacaa gatgccagca
     5221 gctgatcggc agtaatcggc acgtcttgtt cgcgcatcac ttcaagctga tttttacggg
     5281 agccgagaat gtcgttgaat accgccattg ccgtatccgc aaggcctggg tgagcaatcc
     5341 atgtgccatc gtgaccgtta ttggcttcca gcgctttatc cgcttttact ttgttgagca
     5401 cctggttatt gcgctcttca tctttgctcg gaataaacgc tgccatgccg cccatcgcaa
     5461 aagcaccgcg tttatggcag gttttaatca acaggcgtga gtaagcattc aggaatggtt
     5521 tatccatcgt cactgcctgt ctgtctggca ggacgcgatc gggatagttt ttcaacgttt
     5581 tgatatagct gaagatgtaa tcccaacgac cgcagttcag accaacaata tggtcacgca
     5641 gcgcgtgaag gatttcatcc atctggaaca cggcgggcag cgtttcaatc agcaacgtcg
     5701 ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag acttcgctcc
     5761 accaggccgc ttcctgccag gactgggttt tcggcagata gaaataggga ccgctgccct
     5821 ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac aggctaccgg
     5881 ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg cgtacccgac
     5941 aaatcaaaac cgctggattg ggcttgagct gataaatttt gcctgcttca ttggtatagc
     6001 tgatggtgcc gttaaccgca tcgcgcaggt taatttgccc gtcgatcact ttgttccagt
     6061 ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg ttcagtgcgt
     6121 tgatcaccat cttgcgctca accgggccag ttatctcgac tcgacgatct tgtaagtccg
     6181 cgggaatacc acgaattttc cagtccgtat cgcgaatgga agctgtttcc gaaataaaat
     6241 caggcaaagt tccgttatca atatcttgct gctgctgaat gcgcgctgcc agaagtttat
     6301 tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct tcggcagtaa
     6361 gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc cagttcatcg gttgttgttg
     6421 cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccca ctgtgaacga
     6481 aggatcgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa atgcaaaatc
     6541 aaaaacaatt tccattttaa ttaaaaatac ttgataactt attgataaca atatgaataa
     6601 aatttattca cctcctgagg tgcgtttcgg aaataaaaaa ggcacccgaa ggtgcctgag
     6661 gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag cgttggattc
     6721 atgtgccgta gatcgtatgg cgtgatctga tagacgtaat agttgagcca gttggtaaac
     6781 agcaaattac cgtgactacg ccagctcgct cgcggtgtat tttgcggatc attgtgcggg
     6841 aaatagttat acggtacttc cgggcctagt ccggcttcca catcgcggaa atattcctgc
     6901 gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg cttatctttg
     6961 ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag atcggtgtaa
     7021 tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gtggtgccag gaatgaatca
     7081 tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta aacgccagag
     7141 agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc ggcctgtacc
     7201 gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag cacctgtttg
     7261 atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc accggttaca
     7321 atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa gttgttcaga
     7381 tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg aatatcgacc
     7441 tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat cttcttcggc
     7501 atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc acgagaagtt
     7561 gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc gtccggcaca
     7621 cgaataggca taacctgatt acctcactac atacgcttat acgtttagac atccagatag
     7681 ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg agaaagtttc
     7741 aaggaaaagg ctacgttaga atataagaat gacgaaaagg agagaaaatg gttattagta
     7801 ttcgccgctc acagcatgag gaaggggagg aactcgttgc gatttggtgt cgatctgtcg
     7861 atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag ctggttcgtt
     7921 cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggatcag ccggttgggt
     7981 ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat gtgcgcggct
     8041 gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa ctgacaacca
     8101 acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt tttaaggtta
     8161 cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat ctggcgtatg
     8221 tgggggaata agccgcccct gccgtgcctg cccaggctgg agaagacgcg ccag
//
LOCUS       DAZGCS010000164         8219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGCS010000164 DAZGCS010000000
VERSION     DAZGCS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.167"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..1780
                     /gene="iucA"
                     /locus_tag="ACHK01_004532"
     CDS             56..1780
                     /gene="iucA"
                     /locus_tag="ACHK01_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO8099352.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1781..2728
                     /gene="iucB"
                     /locus_tag="ACHK01_004533"
     CDS             1781..2728
                     /gene="iucB"
                     /locus_tag="ACHK01_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO8099353.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2728..4470
                     /gene="iucC"
                     /locus_tag="ACHK01_004534"
     CDS             2728..4470
                     /gene="iucC"
                     /locus_tag="ACHK01_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO8099354.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4467..5804
                     /gene="iucD"
                     /locus_tag="ACHK01_004535"
     CDS             4467..5804
                     /gene="iucD"
                     /locus_tag="ACHK01_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO8099355.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            5810..8005
                     /gene="iutA"
                     /locus_tag="ACHK01_004536"
     CDS             5810..8005
                     /gene="iutA"
                     /locus_tag="ACHK01_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO8099356.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1929 a         2107 c         2206 g         1977 t
ORIGIN      
        1 gagaatcatt attgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac
       61 cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat
      121 tcgtgaaact acagactgga aactgacaga atacccgcca gacgaattga tcatcccgct
      181 ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg
      241 ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac
      301 tctctcccgg cttattattg ataagctacg gcatcaactg tttctgcccg ttcccctctg
      361 cgaaactttc caccagcgcg tgctggaaag ccacgcccat acgcaacagg caattgatgc
      421 ccgtcatgac tggaccgccc tgcgtgaaaa agcgttgaat tttggcgaag ctgagcaggc
      481 gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg
      541 ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt
      601 ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg
      661 atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt
      721 cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt
      781 tgctaagggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc
      841 ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg
      901 gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc
      961 acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt
     1021 aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag
     1081 tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ccaacgtact
     1141 ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa
     1201 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc
     1261 atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct
     1321 gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat
     1381 ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat
     1441 tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct atttccctta
     1501 ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga
     1561 cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt
     1621 gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa
     1681 ctttttctgt tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta
     1741 tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat
     1801 tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg
     1861 actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga
     1921 tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc
     1981 cgaatggtgt gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat
     2041 ccatcgtacc gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc
     2101 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc
     2161 gcagggtgag gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat
     2221 tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga
     2281 gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct
     2341 caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt
     2401 tgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga
     2461 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag
     2521 ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt
     2581 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     2641 gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca
     2701 tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt
     2761 cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct
     2821 cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt tatcgctgaa
     2881 cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca
     2941 gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact
     3001 gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag
     3061 gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc
     3121 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag
     3181 gcgctggaac gatatgcgcc agagtatgcc aacaccttca gactgcactg gctggcggta
     3241 aaacgtgaac atatgatctg gcgctgcgac aacgatctgg atattcagca gttgttgact
     3301 gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg
     3361 gatcacaact ggctgccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccacc
     3421 gacttcatcg ctgattttgc cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     3481 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat
     3541 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat
     3601 atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc
     3661 ctggtgcaaa gcggcgcagt gatccttggt gaaccggctg caggctatgt gtcccatgaa
     3721 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     3781 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc cggttctgat ggcaacactg
     3841 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg
     3901 gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg
     3961 ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa
     4021 gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa
     4081 gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgtcac atcccgtctg
     4141 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     4201 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     4261 gcagtgttga gtgattacat gaacaaacat ccacaaatgt cagagcgttt tgcgcttttc
     4321 tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg
     4381 gaccaggatg gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg
     4441 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga
     4501 cagggccatt taatctcagt attgctgcat tgtcacatca gatcgaagaa ctgaactgtc
     4561 tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     4621 atatgcagac tgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca
     4681 gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcagactac
     4741 gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata
     4801 acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc
     4861 tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     4921 aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa
     4981 tgaatcttcg tcggccggac ctgagtggaa aacggataac tgtggttggt ggaggacaga
     5041 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     5101 actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     5161 attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt
     5221 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     5281 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     5341 ccagccgctc ggtaacaact ctggaaagta gtggtccggg ctggaagtta ttgatggaac
     5401 atcatctgga tcggggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     5461 accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata
     5521 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata
     5581 acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc
     5641 tgatggcctg gagatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc
     5701 tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg
     5761 aggctgctgc tttaactcac tatacaacaa atattcagga ataacgatga tgcgcaaaaa
     5821 gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca
     5881 gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga
     5941 gatggcgcaa accacctggg ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg
     6001 taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag
     6061 ccgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt
     6121 gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat
     6181 cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg
     6241 gttgatcaac atcgtgacca aaaaaggcca gccggaaacc atgatggagt ttgaggctgg
     6301 cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc
     6361 cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg
     6421 gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc
     6481 caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca
     6541 actgataacg cagtactata aaagtcaggg ggacgacaat tacgggctta atctcgggaa
     6601 aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga
     6661 ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct
     6721 gagacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt tctacccgtt
     6781 cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga
     6841 ccagtacggc atgaaactga ctctgaacag ccaacttatg gacggctggc aaatcacctg
     6901 ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca
     6961 ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc
     7021 atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt
     7081 taccgtcagc ggtggcgtac gctatcagta tactgagaac agggtagatg attttatcga
     7141 ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg
     7201 tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga
     7261 acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata
     7321 ttatggtcgc ggcatctatg gtgcagcagt gaacggccat cttcccctga caaagagcgt
     7381 gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg
     7441 ctttaccggt gacaacctgc ggactcaaat cgcagcatat tactcgcttt ccaataagag
     7501 cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg
     7561 cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt
     7621 caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga
     7681 atcaagtcca tcgaaagcga cagcttacat tagctgggcg ccggaaccgt ggagtctgcg
     7741 tgtgcagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta
     7801 cactaccgtc gattttatca gtagttggct gcttccggtg ggaacactca gcttcagcgt
     7861 tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta
     7921 cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg
     7981 tctgaactac tcagtgctgt tctgacgggg attcttttgc aacaagggta tgctgtaaac
     8041 aacatggtcc ttggtggcca tgttgtttac agccagtata atatacagcc ttaacaagat
     8101 attccgtctc cggaatggac tgttcaccac tgaataacaa tatgtctgtt ttatggatct
     8161 ttgactgaaa tttatcgcat atcctcattt cattgtccgt ctgaaaacta accttacaa
//
LOCUS       DAZGCS010000165         8209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGCS010000165 DAZGCS010000000
VERSION     DAZGCS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.168"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..817)
                     /gene="espL2"
                     /locus_tag="ACHK01_004537"
     CDS             complement(<1..817)
                     /gene="espL2"
                     /locus_tag="ACHK01_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO8099357.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVFFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATH
                     KRVFFCTNNICDIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLP
                     DNILQGVVINFAMGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLL"
     gene            complement(808..1372)
                     /locus_tag="ACHK01_004538"
                     /pseudo
     CDS             complement(808..1372)
                     /locus_tag="ACHK01_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1537..2496)
                     /locus_tag="ACHK01_004539"
     CDS             complement(1537..2496)
                     /locus_tag="ACHK01_004539"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO8099358.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            2506..2739
                     /locus_tag="ACHK01_004540"
     CDS             2506..2739
                     /locus_tag="ACHK01_004540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099359.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(2671..2874)
                     /locus_tag="ACHK01_004541"
     CDS             complement(2671..2874)
                     /locus_tag="ACHK01_004541"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO8099360.1"
                     /translation="MVLSDCYSLANDQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            3052..3225
                     /locus_tag="ACHK01_004542"
     CDS             3052..3225
                     /locus_tag="ACHK01_004542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099361.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            3797..4346
                     /locus_tag="ACHK01_004543"
                     /pseudo
     CDS             3797..4346
                     /locus_tag="ACHK01_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            4523..4750
                     /locus_tag="ACHK01_004544"
                     /pseudo
     CDS             4523..4750
                     /locus_tag="ACHK01_004544"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(5277..6647)
                     /locus_tag="ACHK01_004545"
                     /pseudo
     CDS             complement(5277..6647)
                     /locus_tag="ACHK01_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6667..7014)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004546"
     CDS             complement(6667..7014)
                     /gene="tnpB"
                     /locus_tag="ACHK01_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8099362.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(7011..7685)
                     /gene="tnpA"
                     /locus_tag="ACHK01_004547"
     CDS             complement(7011..7685)
                     /gene="tnpA"
                     /locus_tag="ACHK01_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO8099363.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            7939..8209
                     /locus_tag="ACHK01_004548"
                     /pseudo
     CDS             7939..8209
                     /locus_tag="ACHK01_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2016 a         1878 c         2046 g         2269 t
ORIGIN      
        1 aaagcaaccc aggaacatta ttaacatttt ttgattcaca caagattttc atctgagatt
       61 ttgtaagatc tgtaaatcga gtatcgttat ataccttttt tattatctct cttagtcctg
      121 ctcccatagc aaaattaata acaacacctt ggagaatatt atcaggtaat ttttttataa
      181 acacattatt gttgtcattc gattttgttt tatccacaaa aagtgacagt gtgtcttctt
      241 ttagcccata acatttcaga cattgctcac taagaaaatc atatgctgtc aggtttatta
      301 tatcacaaat gttattagtg caaaaaaata ctcgtttatg cgtcgcggtc tgattgggat
      361 catagaaatt aataacaaac ttattttcct tgtcgttttt tattttaatg cccagagcca
      421 ttgcatgtcc gggagaatga acttctaatg atgtgtgaga tttattctct ttctcaagtt
      481 gaagaaaaag gttatatata acgcttcccc aagattcatt ttcaacaaaa aacacatcac
      541 ccttaatatt atttgtcccc ttgttttcct gaattacaat agctttgctt aataattgag
      601 gggatgaaaa tgtttcgtag tctatctttc ctgaattact acaaaaaaac tcactccaat
      661 atgatgctat atgtcgacac catatgattt ccccattgca atcactaacc tttccattta
      721 gatttatact ggcatctctc ttttttgata aataaggggg attgtttttt gaaatatatt
      781 caacataatt tgatgccgat ttgtttatta ttggcatatg tttgtatcct ttctataaat
      841 tttccgcctg acagtggaaa taatatattc tactgatttt tgtagtttaa acgatttatg
      901 aagaatttta ctgttgtaca gtaccagata ctcagccagt ttcagattga acatattcag
      961 ctattcaaag agcaattctt cattaaactc aataaattgt tccctcagtg ttcggttaaa
     1021 atgctcactt atggcattta attttcggcg tatagagata agtccatacg aacccgacgg
     1081 tggcttcctg tagcgttttg tcgaattttc ccgagaactc tttgcccatc atcagtgatg
     1141 atctggttga taccaacaag gaacaactgg gctacacagc agaagaaatg cctgatgata
     1201 tcacttttaa gcgatagtac ggccagagag tagttgctgt gttcgtcgag catggtaatg
     1261 aaataacggt acagctcgcc attctgagct caatagtatc catgccactg agcttacagg
     1321 tctttatcgg gcgatagtgc tttattcttc ggggctttgt ggatgatttt tcgaaatgtt
     1381 catgatagtt aggtaaagct accgcatatc atttgcaaaa tgacagatag atatacttca
     1441 atttgaatct taacaattag tatgggaatg ctgtcaatcc tctgtatgcg tccaccacat
     1501 attgaaattt ttgttctcta atatattcca cgggcgtcaa gagtgtgagg cctgtgctgt
     1561 tatacagcac cagacgttgt cccccatctt tgacctgtct cttgagcaga tctcctgatc
     1621 aagagacttc atcaccaggt aaccctcagc catatcctga tgtctgaacc agccatccca
     1681 cataactacc caaccggggc tgcttgtgcg tttgctgtca tgccatcgcc ccagtttagc
     1741 cagtttcaaa caggcccatt tcagggtcgg tgtttgcgcc ggaagctgct ttccttccag
     1801 cttaacccac agtagtttcc actctgtcgg cgtcagtatt ttctcacagc tgtcattttg
     1861 tgtttcttca ctgatacctc cctgccgcag gcccagcacc cgcaccgcga taaccaccat
     1921 gcgctcaagg ttatcccggg tctgcattcg cagcgattcc acacatgtac caccactttt
     1981 ccacgctttg tggtattcct ctatcagcca gcgttgctcg taatggctga cgatacgtcg
     2041 cgcatcggcg gcactcgcca ctttttctga tgtcagcgga taccagcagg ctccgtcctc
     2101 tcctgctccc cgcaacagac atacgtgagc gagagcgcct ggccgctgtt gtcgggattt
     2161 tttatgctga cttcgctgta actgatgagc atccgggcct ggcgggctgc ccgcccgcct
     2221 ttttgcatca cattcagcgt gtggcttccc gcggctgact ttataatgca ggtaatgcca
     2281 gatatccgct tcccggtcac agacagtgat tacctgtttc tgtatctccc ccagccgttc
     2341 ggccatgcgc tccgaagcct gctgccagcg gtaactttct ttttcttcac agggacgttc
     2401 ttttcgctgg tgcttaacac cgtaggtgtc cgtggcacga ctccagcact gctgttcgat
     2461 aagatcgact ggcagggcgc tgtcgggggc gtacatcagg acagagtgag ccagcagccc
     2521 gtgcgtcttc gggttagtgg tggtattccc caggtcatca aatgccgtac tgtgaccgaa
     2581 gttaatggtg gtggtatctt ccagcgcgag gagcagcgga tgtgcctcac atgcccttgc
     2641 agtggcagta aatccggctt cggcaatggc ttacggggac accgacgggt tacgtatcag
     2701 gcggtacgca ccttcaacct gagcagtgaa gtgggatgat ttcacaatag aaagacctgc
     2761 atgctgagcg agagaagagg tcagtgacac aaggcgtcgt gtacgacgcg gatcaccgag
     2821 acgggcatgt ccagactgat cgttagccag tgaataacaa tcagaaagta ccataacaga
     2881 cccgaagaaa atgaaatata agagaagatc aacgggtgaa gaaaaagttc aaaaaatcgc
     2941 tgccggggag gaaggaaact accggatgga aagagtcccc ctaaagcaga ctgacagaca
     3001 tcacaaatcc ccgggtgggg atttgtgtat aagagacagg ttacaggtgt ggtgaataag
     3061 actgcaaatg tggcagccca tgccgtggtg aatgctgcac tggcggtggt acagggaaat
     3121 aacgcccgtg ccagtgccgc aggtgctgcc actggagacg tggtggggat gatagccaca
     3181 gagatgtgcg gaatatcccc tggcactgtt acatgtaaaa gataaataac gaagctacat
     3241 ccttctcttt atgaacggct ttatattagt tttgctgaca agattgagct aaatccggcc
     3301 aatgaagaag agcaggttat tctttaaagg tttgataaca tgtaacacat tttgaatatt
     3361 cagtcagtaa gccttaaaaa tttacctggt tatgggctga ctgatataac aaagtaaata
     3421 ttcgatgggg aatgccggat gcaatgaaca atgcattaac gtatcgaaat aatgatgcag
     3481 gagcaaactc agtctggtga gtatacatta tgccattgat gcagagttta aaggaacagg
     3541 gctggttcgt tatggaacaa catttgtgcc tgaggaaagg gggggagtat ctgaggcaac
     3601 agaagtttgt ggggagttcg gtttattacc atgcttggtt ttttgtcagt gtgttcaaat
     3661 cgtcaggtga tatatttata gttttttgat gatgatttca aataatgttt ttgagatgtg
     3721 tggtcctttt tgattttcag aaatcattta ttaatgaagg agggttaaat gaaaaaaact
     3781 cgttataatt tcagtaatga tattatctgt tatgagtggt tcaagactgg ctgataatca
     3841 tacgctatcg gctggttatg ctcagagtaa ggtacaggac tttaagaata ttaaaggagt
     3901 gaatctgcaa tatcgatatg agtgggattc tccggtcagt gtcgtcggga gtttttcgta
     3961 tatgaaaggt gattgggcgg acagtcatcg cgatgaagct gacgattttt acagacatca
     4021 ggctgatatt aaatattatt catttctcgc aggcccagct tatcgtctga atgactatat
     4081 cagtttttat ggtttggtgg ggatatctca tactaaagcg aagggagatt atgagtggag
     4141 aaacagtgtg ggtgctgatg agtctgatgg atatttaagt gagtctgtat caaaaaaaat
     4201 ccactgattt tgcttatgca gctggtgtta ttattaatcc gtggggcaat atgtctgtaa
     4261 atgttgggta tgagggaact aaagcagata tatatggaaa acattcggtc aatggattta
     4321 ctgttggagt tggttatcgt ttttaatcat tagacgggac tggataattg cacttgcgta
     4381 gtgaagttta ctgaatgttt gtattttaat gaagtattcg acatattccc ttcagacgga
     4441 atatgtcgac ggttatggtt ttactggagc atgagtatgt tatggcgaaa ctgataaata
     4501 tatcaggtcc actgaggaga gaatgacgcc aggggagagg agggtggctt cacgactgga
     4561 gagctttctg aatgatgatt gctttgtttg gtacgatatc ccggtgggac ggaaaaaccg
     4621 acatccggat tttgtcatta ttgacccgga taacgggctg gtatttctgg aggtcaagga
     4681 ctggactgta tccacattac gtaaagcaaa tcaggagcag gttacactgg aaaccgacgg
     4741 tcttctgaaa tcagaaataa atccactggt acaggttcgt cgttatgcct gtgatacggt
     4801 taatgcctta cctgctgacc cgtgcttacg gcagaatgac ggtcagtata aggggcggct
     4861 gaacctggcc tgggcgtatg gtgtggtgtt tacccgcatt acccgtcagc aactgaaggc
     4921 actgacaggg aataatgaga atgcggtgga aaagatttct ccttcagcac agacaatctg
     4981 tcaggatgaa atgacgcagt ctgttttacc tgaggtattc cggcaaaaaa ttgccgggat
     5041 gtttaccacg gggttcagaa cccgggtaac accccggatg agggatattc tgcgcgccca
     5101 tctgtttcct gaagtgaccg tgaaacagaa ctcacaaatc aaaattatgg atattcagca
     5161 ggagattctg gccagaaata ttggtgacgg gcatcgtgtc attcatggtg tggccggttc
     5221 cggaaaaacc atgattcttt tgttccgttg cctttacctg gcagaaacca cgtccggtag
     5281 ttacgccgcc ccagtgcaac tacccggagg gcgttttcac acaggttatt gtcgatctcc
     5341 acccagccat tgcggcagta ttcgttcaga gcgtcccatt gcttcagcag ataggtgaac
     5401 gctttcgctg tatccgagtg gcgcgacagc gtgctcatct gcccctggag ccactcatac
     5461 aacgactgca ttagcggtac cgttctggct tttctgaccg ccagtcgctc ttctgccgga
     5521 ctgccgcgga tctcagcctc gatagcgtac agttccccga tacgacggag cgcttctccc
     5581 gttactgtcg ttggatggcg gacatgtaca tcgtggatct tgcgtcgggc atgtgccata
     5641 caagccgctt cggttacctg accgctttcg taaagagcat tgtaacccgc atatgcatcg
     5701 gcctgcagga tacctctgta gtccgccaga tgttgctgtg ggtggatgcc tttgcggtcg
     5761 ggagagtatg agaaccacac cgctgccggc atggtcgaac cggcgttgcg atcgtcacga
     5821 acatagaccc acagccgtcc ggtacgggtt ttaccctgac ccggctccag tacatttacc
     5881 ggcgtgtcat cggcatgcac tttacccggc atcagcacat agtgcttcag ttcatcatac
     5941 agcgggcgaa gctgctctcc catgatgtca acccagcgcc ccatcgtatt gcggtgcagc
     6001 tccacgccct ggcgggcata gatttctgac tgacggtaca gcggcagatg ctcggcgaac
     6061 ttagccatga ttatgcgggc cagcagagcc ggactggcgt aactgcgctc gatgggtttt
     6121 ggtggctgcg gagcctgaac tatacagtcg caccggctgc aggcccgttt tgggcgaacc
     6181 gtttcgatta ccctgaacgc ggtgttgatg atatccagtt gttcagagat gctttctccc
     6241 agcggtttca gtttgccgct gcagacgggg cattcggttt ctgccgggga gataacctgc
     6301 ctgtcacggg gaagtgttgc cggaagtgct ttgcggacgg gagagtctga tgttttcggc
     6361 gctgtctctc cggccattga ggtgagttgc aactgcgcct caccaagcat gttctggagc
     6421 tcggttatac gcgcttctgc ccgtgcgatc ttcttttcta tcttctcgcg gcttttctcg
     6481 ctgctgcgac cgaacaacat tctctgcagt ttagcgacca gcgctctgag tgagctgatc
     6541 tcgcgggcat agccggttat ttcaccagac agacggacga taacagcctg ctgtgcgatc
     6601 agcagggcct tcagttgctc gatgtcgtcg gggagtgtgt tgttcattcc cctgttttat
     6661 cacgggttat atccggatgc caggccgttc tgtccgtttg ggatgttgcc atgcgatccc
     6721 ctccagtagc atggataact gagctggcgt caggtgcact ttcccttccc gggttaccgg
     6781 ccagacgaag cggccccgtt ccaggcgttt ggcgaacagg cataacccgt cacgatcggc
     6841 ccacagtatt ttcaccattt tgccactgcg gtcccggaag acgaagatat gcccggagaa
     6901 cgggtcatct ttcagcgtgt tctgcacctt cgaagccagg ccgttgaagc cacaacgcat
     6961 atctgtgatg ccagcgatga tccagattct ggtaccggtt ggcagcgtta tcatcgggta
     7021 cctcctttca tttcgcggat tagcgcccgt aacatttccg gagtgagagg gtcaaacagt
     7081 tttaccacac ctgatttaag atgcagctcg caccgtggga cgtttccggg atcactctca
     7141 gggcactcat caggcttgtt acgccagaag ggatttgtaa ctggtctggt cggctctggc
     7201 gtatcagtca gagccaccgg gacaggcatg cattcctgta tgtcatcatc gctcagtaag
     7261 ccgtcctcgt actggctttt ccatttaaac agcaggttat cattgatacc gtgctctctg
     7321 gcgatccggg caacaacagc accgggctgt aatgcctgct tagccagacg gaccttaaat
     7381 tcacggctgt agctggctcg ccgttctttt cgccatgtgc cttcgctgat ttgaggctct
     7441 gttaattcct tctttctgtt ggcataaagg atggcgtcaa gctgagctaa tgaaactgaa
     7501 tcgggcaatg gccatgcgat accggatgca ataaatcgct gaaaaagcgt atgtattgtg
     7561 gaatgactga gacctagacg ctgagcgatg gcccggatgg tcagtttatc ttcaaatctt
     7621 aaacgcagag catcaggcaa ataagaacgg aagcagggaa tatctttttt tgtctgggaa
     7681 ttcatcgttc gtgtccatct atatagatgg gcgcgattgt tgccagacag gacaattttc
     7741 acaagacgtc gcagatgggg cgcttacaca acaatggcgc gagggaaagc tgctattacc
     7801 ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga
     7861 atcgctcgca gaggacccgg aaaccctcag tatcagctgt tgtcaacgac ggatgactat
     7921 tgcaccgttc gtgacagtgt gctgcaagcg gatgcgtacg ccgggttcaa cgagctgtat
     7981 cgcaatggtg ggataacgga agctgcctgc tgggctcatg cccgccgaaa gatccacgat
     8041 gtgcacgtcc gcatcccgtc agcactgacg gaagaagccc tggagcagat cggtcagttg
     8101 tacgccatag aggcggatat aaggggaatg ccggcagagc agcggcttgc tgaacgtcag
     8161 cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt tgcgtgaaa
//
LOCUS       DAZGCS010000166         8088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGCS010000166 DAZGCS010000000
VERSION     DAZGCS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.169"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..891
                     /locus_tag="ACHK01_004549"
     CDS             <1..891
                     /locus_tag="ACHK01_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO8099364.1"
                     /translation="KIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIK
                     DLAKNITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNAS
                     RMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLE
                     GSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPS
                     IKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQK
                     IDNISLDEFAKALKSVLARI"
     gene            complement(1153..8088)
                     /gene="yeeJ"
                     /locus_tag="ACHK01_004550"
                     /pseudo
     CDS             complement(1153..8088)
                     /gene="yeeJ"
                     /locus_tag="ACHK01_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         1892 a         1929 c         1980 g         2287 t
ORIGIN      
        1 aaaattaagc atcttttaat tgttacgtcg acaattaatt tagaagtgtt taatgcactt
       61 aaacttgaaa agactaaatt aacattagtc acaatgacta tgcaggatca cgcaacatta
      121 aaagaaataa aagatttagc aaagaacata acacagaaat acggtacgcc ggatctttgt
      181 atagaggcga tgcgtaaaaa gaacctgaaa acgatgatat ttatcagtca gttgaaagca
      241 aaaacgaatt ttcaggttgt tggtttaacc atgaagtgct actccccttt gtgcaagaac
      301 gcgtcccgga tggatcagaa tctccgggct cctgtaccca tgacatgggc atttatgatg
      361 cgtgaggcgg gttttccagc agtcaggcca atatatgaat tgccactaag tgaggatgta
      421 ctcgatgagg tgcgcgagga aatgcgatcg ttaggatctt acattgcgct taatttagaa
      481 ggtagctcgc aggaacgtac attttcatta tcgattgcag aaaatctaat agcaaaaatt
      541 caaagtgaaa cagatatacc aatagtgatc gttcatggac caaaagggga agataaagcc
      601 agggtattag tggattgtta taataatgtc taccgtttat ccttatcacc ctcgattaaa
      661 cgttcagcag caatcataaa agatgcttat atcgcaataa ctcctgacac ctcaatatta
      721 catatggcaa gtgcctataa tactcccgtt gttgcaattt atgctgatta caaaacgcga
      781 tggcccgcaa tggccgatgt ttcggagtca gtcgtcgttg ggcaaaaaat tgacaatata
      841 agtctggatg aattcgcaaa ggcattaaaa agtgttttgg cgagaatatg agacagtact
      901 tatgcatagt aatgccgatc tgttatgaac ggtggtgtca tcaatactct gaggctggtc
      961 aatcatcaca gaaaaccagc ctttggatca aagctgtact caccggatac gatcatagtc
     1021 acatcgaacg cgattcttgc ggtgcagcag ttttatttat tcattttctg tgagagaagg
     1081 gcaagctcta tagagggaat gtaaaaaaga gtgctccgaa tggagcactc tttaagaaat
     1141 tataacggaa aatcacaggt ttttataaca tgtcgcataa gcaaacccaa gcttttcaaa
     1201 tacgctttga tcacctgttg ccagggaaac tacgtattgt tcgcttgagt ttgcgggact
     1261 agatgaccaa tacatatttg attgaaaacc agcttcagtc gtgtaatgcc ccatatctcc
     1321 ccattcactg tacagtgacc cgatgcctcg ggaacgacta tggtttaaat gatctgcacg
     1381 gggaagtgtg tagccattgc tactgcaaaa attttcagca aggctgtata tcatgaaagc
     1441 atcgccggag ttcacccacc aactcttcac acggatttcg tatacatagc tagggcctcc
     1501 tgattttggc gtcgccgtaa tcctttccca attgctgccg acatttttaa atgtcacttt
     1561 accggtagca tcaacatcga cccaggaggc agagcttgaa aactcataat cagccgccgt
     1621 ttttcctggg gcaaagttgt cattattcaa ctgataatac gccccggtga acccctgcga
     1681 agggaatgta gttgtcggta ggtcagtacc attgactgat actgtaccgc tcattatttt
     1741 gtcttctgcg cgagtgaatt gtattgtggt actcagacca gcttgatgaa caccattcaa
     1801 tacagggatc agcgttgcga taccctctcc gccgcctgta atggtggctt tgtaatcacc
     1861 gttgatattt tgactgtaat caattgcgct aattttcata tagggcacgt tagtacctga
     1921 ttgcacaaat tccatccctt cagaaacttt gatcggattg cctgatatat cgtgcaaaac
     1981 aagatgtagc tcagcactat cggtaaagtc ccctttcagt gaggaccgat tgttcttaag
     2041 gacggactgc gaggcgtctg ccgggcctgc caccagcgtt atatctactg tttgcattcc
     2101 accagcggtc gtgactgcgc ttaccgtgac gctacctgtc atcgctcctt tcacgcttgt
     2161 tgtcgcgatt ccgttttgat cggtcaccgc tgttaatgac gttaatgtgg cagagccgct
     2221 ttttaaggcg aagtacacag tgagaccttc gatcaggtta ccactgccat cctcaaccgt
     2281 tgcctttaag gtacttaaat cactgttggt ggcggcgata gtcgatggat cagcgataaa
     2341 gctagcaaca tgtgcggtgc ttgagttgcc agtgactttc acggttgtct gtgtctgtat
     2401 tgttacgccg ttatggaacg atgcagtcac cgtatatgta ccgactttat tgctggtcag
     2461 aacgaccgga gcctgacctg aagagttagt tctcactttt ccgccactta acgtcgcccc
     2521 ttctggcgtt acgctaaagt tgatgacctg accctccact ggagtgccat ttgcagaggt
     2581 tagcgttgcc gtcagagtag tgcctgtagg ggcatagaca ccgataagcg gactggacgc
     2641 cgtgagtgtc agtttttcat caatagcctc cagttgtttc tcaagtgagg ctccattcgc
     2701 cagggatgct ttcaccatat acgaaccgtt tctttcgggc gtcatggaga cctcggctat
     2761 accatgcgta tcagtagaga caatattttg gctgatggtc atgtgctctg gtggctctgc
     2821 actgaatgtt acagattcat taagaatcgg gttgccaaac tggtcgttga cgtgagcttt
     2881 aactgctacg tcttgtgcga ccattagctg accttcaggt atctccagac tggtaatcgt
     2941 tgcagaatta atatctgctt ttgcattcag taatcgcgat atgacttcag taggtttatc
     3001 tgccagagag gctgaaactg ttttcagtcc gacttcggtg cttgtcacaa gaatttcagc
     3061 gaaaccctga tcatccgttt caacgctggt gctgcttagc gtgacggagg ttccgcggaa
     3121 attaactttt atgccttcta ccgggttgcc gttagcgtca gtgacgcttg cagtcatggt
     3181 cgcgccctcg gtcgtgctaa cgacgaatga gtatacagag gttaaggagg ctagtgttgc
     3241 ggtaccagca tcggcaatca aagtcacctg tttcgtatca ctgacaccat aattgatcac
     3301 gctaactgtc acgggatagg tgccggattt ggtaccgctc agtgtaactt cagccttgcc
     3361 gttgatatca gtaatggcgg aaccgccttg tccgagagtg aagcttgcgc tcacatctgc
     3421 cggtagcgtg aatgtcaccg cctcattggc gaatgggttg ccgtttccat cagtcactgt
     3481 tgcctgcagc ttggtcatcc caatgttatt ggcgatgaaa ttgtcctcgg taacgttaag
     3541 attaacctgc gcagtgagcg tatccgcagt aaagttcacc accagttgtc cactcttact
     3601 gccagccatc gatgctgtaa cagtatgagc gcctgctttt atacctttca gcgtgacttt
     3661 cgctttgcct tcagtatcag taatcgcttt accgccatcg ctcagcgtga agttcgccct
     3721 cacatcttcc ggcagagtaa atgttacctc agtgttggct atcgcattgc cctctgtatc
     3781 agcgactgtt gctgttagtg ttgtgagatc gttattgtcg gcaatcaccg gatccttcga
     3841 ggctgccagc gtgatttcag cattcgcgac gttcggcaca taggtcactg tttgttgcat
     3901 cgaatcgcca ttgtcgaggg ttgccgtcac ttgataaacc ccggcttttg tagcggtaaa
     3961 gcttgcgtaa aaatagccgt agtagttggt agtatatata ccgttattac tgagggtcac
     4021 gccttcactt ggtgaaacgc tgagggttac ctgatgctgg ggtacaccat tgccgtaatt
     4081 atccttcacc tcaatataca gactggttgt ctcgcctgcg gagactgtat caaaaagtgc
     4141 atgtagcaag gtgagatgtg ctgtagacgc atccgcgttg acattaattg atgtgctaag
     4201 cgtggagtta ccattttcca gactggcctc aacggtatcc ggtctcgctc ctgattctat
     4261 cgaggagcgg gtattggtat aagtgacggt tgccttaccc tgttcgttcg ttacggcttg
     4321 gccgccattc gtcatttcgg cagagtttgt tcggctggtg aagttcacag tcacaccttt
     4381 caccggaaag ccattattat caacgactgt ggcggtgatg acgctgccgg tgctgttctg
     4441 cggggtaccg gcgattatgc tgtctgggac aggcgtcaac tcgataattt ttgccgccgc
     4501 tgtgtcgcca ataaaatgca cagttttgtt gtcgctggca ccattattag ccagtgatgc
     4561 cgtgaccgtc tgctcaccaa aggcaacgcc tgcgatagtg gcctgcgcga tgccagactc
     4621 attggtatta ctcactcccg gggtcagggt gagtcctgaa gaggctgagc tgaatgttac
     4681 cggaagatta ttcacctcat tgtcgaactg atctttaacc gttgcagtta gcgttgcgct
     4741 atcgacgcca ttacctgtga tctcatcttt tgatatctgc aggacaacct gagccgaggc
     4801 tttgtccgcc acaaatgtta ccggctgcga atcactggta ttgttattac ccagcgttgc
     4861 ggtaaccgta tgcgtgcccg cttttttacc tttcagcgtg acatgcgctt ccccattggc
     4921 ctgagtgatg gcaataccgt tattttcaag ggtaaagttt gccgcaacgt cctgtggcat
     4981 ggtgaagttg accgttatcc ccgccaccgg atgattcgac ggatctttca ctgttgctgt
     5041 cagagttgtc tcatccacgc cattcccaat gatttccgct ttcgatgttt gcagaacgac
     5101 aaccgctctg tctttatccg ccacaaacgt cattggctgt gtatcgctga cattgctgtt
     5161 agccagacga gccgtgatca tatgcgtgcc cgctaacgtg ccggtcaggg aggcgattgc
     5221 agtcccgttg ctatccgtca tgccttttcc gctgttgcta atcgagaacc gacttgcgac
     5281 gtcgtttggc acagagaagg tgatttcttt atcttttaat gggttgccat ttttatcctg
     5341 caaggttgca gtcatatgta gcggagctgt gttggtgacg gtgatatcac ctgaaggcac
     5401 actgagggtc agggcagcag tactttgatc accgataaaa atcacctgtt gattagcctg
     5461 agaaccagag ctcacagagg ccgtaaccct ataatcacca tttttcaaac tggtcagcgt
     5521 agctgtggcg atcccgtcgt ggctattcac ttcggtttgg ctcagtttcg ctgctgctga
     5581 attaacattg aaagtgacct tgacgtcatt gagcaggttg ccttttgcat cccggactgt
     5641 cgcggtcatt gtggcgctat cattgccgtc agcgaccact tcatttttcg acttctgcag
     5701 atcaacctgc gcggtactcg agtcgccgac aaaactgaca attaacgttt gtttcacgcc
     5761 attttcaagg gtgacttcaa ccgtgttgtc ttcctgctta ctacttttca gatcaaaagt
     5821 cgccagacca ttaacatccg tttttgcggt gttttgattg ttaaagcaag ttgccgaccc
     5881 gcttaatacc gcaaacgtga cggtatgatc attgattggg ttgcttcctt catcagcgac
     5941 attgaccgag acggtgtttg ctgcattctc attggcgagc acaccattat tgctggcaga
     6001 taatgtcgca atttttgctg actgcgggtt ggcgtcgatg ataaatccag cggtatgcaa
     6061 atcttcattc cagttttgca ttaacagctt cgcagtaagc ccactgcctc tggtataggc
     6121 ggtataggtt gccttataga cgccatctgc ggtttctttc cagtctgtag tgacaccagg
     6181 tttcacgttg tcgatgctga ctgcgttatt cagttgctgt ttttgctcct taacaggttt
     6241 gtcattttca tctctcagtt ctaccgtcac ctcgatagga ttgccggaga gataacggtc
     6301 tttatcaatt ttaattgacg agtgagttcg ggatgacgaa atagaaatga tattcaccac
     6361 ggcgggggct ttagccgcat ccacaccatt cagctgtggc atcagcgtca gcgtgccaga
     6421 catcgctcct gtggtcagga tctgggtata gcttccgtca ccattatctt tccattcaga
     6481 aagggtgatg tcctgaacac cttcgtgacg cgtcgagagc accagcccga caacaggatt
     6541 acctgctgca tcatgcgcaa taaaagtcag tgtggcggtt gaatgggaat ccgcgttcaa
     6601 tgtttgggta cttaacgata ccgaggaatc tttctggctt agcgtaggtg cctgaacgac
     6661 caccatgctt tgttcacgat tcgacaaatt gcctttgaca tcttcagcgg tgacttcaat
     6721 cggccaggtg ttatcggttt ctggcgtact ggtgaaccgg taacccggca gggtaaccag
     6781 aatatcttta cccgttgtga ccactttgcc accggcagct tccagtgcgg tggcttcgac
     6841 gttatagcct ttcagggcat atttggtttg tagcgacgaa accagtgatt tcacttctcc
     6901 tgacttccct gtcacggggt ctgtcagggt caggcggacc agttcttttt tgcgatattc
     6961 cagaacgatg ttgttgttgc gatccaccag atcataacgg ctgcctgcaa ggctacgccg
     7021 tgcagcgact tcattcgggt caagctgttt ctgcattgcg ctgccaggtt gccaggtaaa
     7081 atcgacggca aaacgggtgt cattttcgcc ctgtttaccc tggcgttgct ccgcgctgaa
     7141 ggtcattagc gggaaggggg tatagttaag tccagcggtt atggcatgag gattactttg
     7201 ccgatcgtct ttatcgaaca gggccacttc atcgccataa tactgttcat agaccagttt
     7261 accgccaagg tacggccagg cgggtagcca gccttctgcg cgtacatccc agccattggc
     7321 cgggcgtgct tcataatcgt tgtccagttc aggtgcgctg cgccagttgg tcagtcgcaa
     7381 atagccgtta ctgcttaatt ttagatagtc gcgccagtac tccgcgccaa tgccggcgcg
     7441 ggagtggtaa cggctaagat cgtggtcgaa aaagaagttg atgcccgaca tccatgtggg
     7501 agtgaaatga cgccaaccta agccgttgtt aatctgtgta cgctcgtcag tacgatggag
     7561 agtatgctga ctgaaaaaga gattatcagg cgtttcatac cacggatgga gaaaatcgaa
     7621 ctgggagttc ttcaggctaa aatcttcatc cacgcccagc gtgattcttg cggtaccgaa
     7681 gcggcttaac cagtctgtca ttgcgcctga agcctgagaa gaggcccatc cacgcgccat
     7741 atttgccgct tgctcgctgt tcatatcctc ggcgagcaga gacccgattt gctgtgaagt
     7801 actggctatc tgttgctcaa ggttgccgct gctattaccc ggtggcgggg ttaaattatt
     7861 ttcactaact tgtgccggga catccagttc atcaccctgg cggacattat caaaacctcg
     7921 agcaaacgta cgaaactggt tgagtttgcg taactcagcc accgaaatac cgaaacgttc
     7981 ggcaacgctt tgggccgatt tccagcgctc caagggtgta gggcaccgta ttggcatttg
     8041 caatggcgag ttgtgcagga actggttgtt gggttgcggc gtttacca
//
LOCUS       DAZGCS010000167         8047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGCS010000167 DAZGCS010000000
VERSION     DAZGCS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.170"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(211..1404)
                     /gene="tyrB"
                     /locus_tag="ACHK01_004551"
     CDS             complement(211..1404)
                     /gene="tyrB"
                     /locus_tag="ACHK01_004551"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO8099365.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1657..2736)
                     /gene="alr"
                     /locus_tag="ACHK01_004552"
     CDS             complement(1657..2736)
                     /gene="alr"
                     /locus_tag="ACHK01_004552"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO8099366.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGIIKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2789..4204)
                     /gene="dnaB"
                     /locus_tag="ACHK01_004553"
     CDS             complement(2789..4204)
                     /gene="dnaB"
                     /locus_tag="ACHK01_004553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO8099367.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4287..5270
                     /gene="qorA"
                     /locus_tag="ACHK01_004554"
     CDS             4287..5270
                     /gene="qorA"
                     /locus_tag="ACHK01_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO8099368.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5436..5678)
                     /gene="pspG"
                     /locus_tag="ACHK01_004555"
     CDS             complement(5436..5678)
                     /gene="pspG"
                     /locus_tag="ACHK01_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO8099369.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5812..6849)
                     /gene="dusA"
                     /locus_tag="ACHK01_004556"
     CDS             complement(5812..6849)
                     /gene="dusA"
                     /locus_tag="ACHK01_004556"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO8099370.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7212..7952)
                     /gene="yjbM"
                     /locus_tag="ACHK01_004557"
     CDS             complement(7212..7952)
                     /gene="yjbM"
                     /locus_tag="ACHK01_004557"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO8099371.1"
                     /translation="MFLIEQINDLKMWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFA
                     INSICNPGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSF
                     DPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            complement(7943..>8047)
                     /gene="yjbL"
                     /locus_tag="ACHK01_004558"
     CDS             complement(7943..>8047)
                     /gene="yjbL"
                     /locus_tag="ACHK01_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO8099372.1"
                     /translation="YAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2098 a         2078 c         1897 g         1974 t
ORIGIN      
        1 gttagatgtg cagaaattat tactccaccc aaaaaaacag gataaggaat tccagataat
       61 taggataagc ttaacaataa aagaactaat aacgtttatt atctctcacg tagaacgatg
      121 gcatcaaaaa aatcatctat aaagaaagag tgaaagcgac gacagtttaa tttcactgca
      181 ggctgggtag ctccagcctg ctttcctgca ttacatcacc gcagcaaacg cctttgccac
      241 acgttgtaca tttgccgtat ttaacccggc gacacacatg cgaccgctgg cgatgagata
      301 gacaccaaat tcttcacgta gtcggtcaac ctgagcggca cttaaaccgg tataactgaa
      361 catgccgcgc tgattaagca gataatcgaa attgcgttct ggcatctctg tgcttaatac
      421 cttcaccaat tcctgacgca ttgccagaat gcgagtacgc atctcttcta cttccgccag
      481 ccagctggct ttcaatgcct cgtcattcag cactgcagcc accacctgcg caccaaaatt
      541 cggcgggctg gagtagttgc ggcgaactgt tgctttcaat tgccccagta cgcggccagc
      601 ggcttcggca tcttcacaca gaacagaaag tccgccgacg cgctcgccgt aaagggagaa
      661 aattttcgag aacgaattgc tcaccagagc gggtaatcca gcgctggcaa tggcgcgaat
      721 agcgtaggca tcctcttcca taccggcacc aaatccttga taggcaatat cgaggaatgg
      781 aataagctcg cgggctttga gaatttcaat caccgcatcc cactgatcat tagtgagatc
      841 ggcacccgtt gggttgtggc aacatggatg cagcaacaca atactgcggg caggtaatgt
      901 tttcagcgtc gccaacaggt cattaaagcg cacgccgtta gtcgcttcgt cataccaggg
      961 gtaagtactc acttcgaatc cagccccggc gaatattgct acgtggtttt cccaggtagg
     1021 atcgctgacc cagacgcctg attccgggaa gtagcgtttc aggaaatccg cgcccacttt
     1081 caatgcccct gagccgccaa gggtttgaat ggttgctacg cgctgttgtt gcagtactgg
     1141 atggtccgca ccaaacagca gcggcgcaat ggcatggcga tagctgttaa gcccttccat
     1201 cggtaaataa agcgaagcgc catgaggctg cgcattcagg cgcgcttccg cctccgccac
     1261 ggcttgcagt tgtggaataa ttccgtcttc gttgtagtac agaccgatac ttaaattcac
     1321 tttgtcgctg cgagggtctt ctttaaaacg ctccataagc gtaagaatcg ggtcgccagc
     1381 gtaggcgtca actttttgaa acacgcgatg gttctccagg tttacgggca ggtggttaaa
     1441 acacaataaa ccggaagaag gcgaagatcg agtggatgtt caggagcgaa cggcaattag
     1501 caacagagtg agactcatga caaacgtaca tccgccagag gcacgacctt tataagagca
     1561 tgaaagaaca tcaacttatt gaatttttag gattttattg gccggataag gcattcacgc
     1621 cgcatccggc acagacaatc aaatattaca gaacgattaa tccacgtatt tcatcgcgac
     1681 ccttgaagtc aggcgcgtaa taagttcgta agcgcttact ttcgtcattt cagcgatacg
     1741 ttctacgggc aaaccttcgc cccataaaat gaccggatcc ccggctttgt cctgcgcttg
     1801 tggacctaag tctacgcaga tcatatccat cgccacgcgc ccgacaatcg gtacttcgcg
     1861 accgttcacc agcactggcg taccggacgg cgcggcgcgc ggataaccat cgccatagcc
     1921 catcgcgact acgccaagac gggtatcacg ttcgcttacc caggttccac cataaccaac
     1981 aggttctccg gctttatgct cacgcacggc aatcaggctg gaggttagtg acatcactgg
     2041 ctgacagcca aaatcggcac cggtggagcg atcttccagc ggcgagacgc cataaagaat
     2101 gatgcccggg cgcacccagt caaaatgcga ctgtggccac agcagaatgc cacccgatgc
     2161 ggcaatggaa cgttgaccag gtttgccttc gcaaaaggta ttaaagatag cgagttgttt
     2221 ctcggttgcg ccacattttg gttcatccgc gcgcgcaaaa tggctgacga tattcaccgg
     2281 ctgacgaacg tttttgcact gggtcaggcg atgataaaac gcctcagcct gttccggcct
     2341 tacgcccaga cggtgcatac cggtatcgag tttcatccag acggtaaccg gctcgtccag
     2401 gctagcctct tccagcgcag ccagctgttc ttcgttatgc acggcggtat gaaaatgttg
     2461 cgcagaaatc gtcggcagat ctctggcatc aaaaaagcct tcgagtaaca gtacaggttt
     2521 gatgattccc cccgcacgca gtcgcagagc ttcttcgaga cgggctacgc caaaggcgtc
     2581 agcatcgggg agcgttcgcg cggtctcaag aagaccgtga ccataagcgt tcgctttcac
     2641 caccgcaacc attttactgg caggggccag ttcacgaaga cgttgcaggt tgtgtcgcag
     2701 agcgcggcgg ttaatcacaa cagttgccgc ttgcatttgt gttccttgat aagtgtttgc
     2761 tttaattacc taattcataa aataattatt attcgtcgtc gtactgcggc cccgcatagt
     2821 tgtcgaagcg cgaccattga ccgttaaagg tcaggcgtac cgtcccgatt gggccgttac
     2881 gttgtttacc gataataatt tccgcgatgc cttttaaatc actgttttcg tgatacacct
     2941 catcacgata gataaacatg atcaagtccg catcctgctc gatagagcca gattcacgca
     3001 ggtcggagtt gaccgggcgt ttgtcggcac gttgttccag agaacggttc aactgggaca
     3061 gcgccaccac cggcacgttc agttctttcg ccagtgcttt cagcgagcga gagatttctg
     3121 caatttccag cgtacggtta tcggaaagcg ccggtacgcg catcagttgc aggtagtcga
     3181 tcatgataag cccgatgccg ccgtgttcac gggcaatacg gcgtgcgcgg gaacgcactt
     3241 ccgttggcgt caggccggag gagtcatcga tataaatatt gcgtttttcg agcaaaatac
     3301 ccatggtgcc ggaaatgcgc gcccagtctt catcatcgag ctgcccggta cggattttag
     3361 tctggtcaac gcgcgacagc gacgccagag aacgcatcat gatctgttct gatggcatct
     3421 ccagcgagaa gataagtact ggtttatcct gcaacatcgc cgcgttttcg acgaggttca
     3481 tcgcaaatgt tgttttaccc atcgacggac gcgcggcgac gatgatcaaa tccgacggct
     3541 gcaagccagc ggtttttttg ttgagatcgt cataaccggt gtttactccg gtaacgccat
     3601 cgtgtggctg ctgaaacaac tgctcaatac gcgccacggt tgcgtcgagc acatcggcga
     3661 tgttcttcgg cccttcgtct ttgtttgcac gactttcggc aattttaaag acgcgggatt
     3721 cagcaaggtc cagcagatct tcgctggtac gcccctgcgg atcaaaaccg gcttcagcaa
     3781 tctcattcgc aaccgagatc atctcacgga caacggcacg ttcacgcacg atgtcagcat
     3841 aagcactgat gttcgccgca cttggcgtat tttttgacag ctctgccaga taagcaaaac
     3901 caccgacgct atcgagttgc ccctggcgtt ccagcgattc cgcaagggta atcagatcga
     3961 taggactacc gctttcctgc aaacgcgcca tttcagtaaa gatatggcgg tgtgggcggg
     4021 tgtaaaaatc gtctgccacc acacgctcgg ctacatcgtc ccagcgttcg ttatccagca
     4081 ttaaaccgcc caacaccgac tgctccgctt cgatcgagtg cggaggcact ttcagcccgg
     4141 caacttgtgg atcgcgttcg cggggttcag cctgctgttt gttgaagggt ttatttcctg
     4201 ccatagtgaa tggagttacc gagataaaga atgggtcgaa actttaccat atgaagcaga
     4261 ccctgacgat acgttctgga ggacacatgg caacacgaat tgaatttcac aagcacggtg
     4321 gcccggaagt acttcaagcc gtagagttca ctcctgccga tccggcagag aatgaaatcc
     4381 aggtcgaaaa taaagccatc ggcatcaatt ttatcgacac gtatatccgc agcggccttt
     4441 acccgccgcc atcactaccc agcggattag gtaccgaagc ggcaggcatc gtgagtaaag
     4501 tcggcagtgg tgtaaagcat attaaggcag gcgatcgtgt agtctatgcg cagtcggcgt
     4561 taggcgctta cagctctgtg cataacatta atgcggataa agcggcgatt ctgcctgcgg
     4621 caatttcttt tgagcaagct gcagcatctt tcctgaaagg cttaacggtt tattatctgc
     4681 tgcgcaaaac ctatgaaatt aaacccgatg agcagttcct gttccacgca gcagctggcg
     4741 gcgttggctt gattgcctgc cagtgggcaa aagccctggg cgcgaaactt atcggcaccg
     4801 taggaaccgc gcaaaaagcg cagagcgcgc taaaagcggg cgcgtggcag gttattaact
     4861 atcgtgaaga ggatctggtc gagcggttaa aagagatcac cggcggtaag aaagtgcgcg
     4921 tggtgtacga ttccgtgggc agagacacct gggaacggtc gctggattgc ctgcaacgcc
     4981 gcggcttaat ggtcagtttt ggcaactcat caggtgcggt taccggtgtg aacttaggca
     5041 ttctcaatca aaaaggctcg ttgtatgtga cacgcccttc cctgcaaggc tatatcacca
     5101 cgcgggagga attaaccgag gccagtaatg aactgttctc tttaattgcc agcggtgtga
     5161 ttaaggtcga tgtcgccgag cagcagaaat atccgctgaa ggatgcgcag cgtgcgcatg
     5221 agattctgga aagccgggcg acgcaaggtt ccagcctgtt gattccataa aagaaatagg
     5281 gcttccacct gggaagccct ttctttttat agttcggctg tatgtagggt acagcacgat
     5341 gaatctgtta gaggcgcaat agtgacagat ttgattatca attcctattt tgttctaagg
     5401 ataaaacctt aggttgtgat catccgcaca atcccttagt aacgccagcg gtcataacgc
     5461 tgatatttcg gcacttttgg tgctttaatc gccttaataa cccacaccac cgcaatcgcc
     5521 agtagtaacc acggcagcaa cttaatcatc aatgccagca taccgccgag gaacataatg
     5581 gccgtcgcca caaccagcgc ggcgataatg cccagcaacg aaacgccggt gaccatcagc
     5641 atgacaaaaa agccaatcac aaaaagtagt tccagcatga tgctctccca aatatgaaat
     5701 ctcttgctgg cattacaaga atcatgccaa aaataatcta ttgatttaac agcaaaacgc
     5761 cccgcgacgg tgcgcagggc gtggtgaatt tgactacttt ttggtgaaaa gttaacgctt
     5821 atccgccacc agtttgagcg cgtgttccag cacattaatg tctgcacccg ctttatgggc
     5881 attttcactt aaataacgcc gccactgccg cgcgccagga ataccctgga acaagcccaa
     5941 catatgccgg gtaatatggc cgagatacgt cccctggctg agttcacgct caatgtacgg
     6001 atacatggcg cgcactaccg ccaccggatc ggcatcggta tccgaggaac caaagatctc
     6061 ccggtctact gccgccagaa tacccggatt ctgatacgcc tcgcgcccga ccatcacgcc
     6121 atccatatgt tgcaggtgtg ctttggcctc ttccagcgac ttgataccac cgttaatcga
     6181 cattgtcaga tgcggaaagt cacgcttcag ttgatacaca cgcggataat cgagcggcgg
     6241 gatttcacgg ttttctttcg ggcttaaccc cgaaagccag gctttacgtg cgtggatgat
     6301 aaacatctca cactcgcctt tgccggaaac agtgttgatg aaatcgcaga gaaattcata
     6361 gctgtcctgg tcatcgatgc caatacgcgt tttcaccgtc actggaatcg acaccacatc
     6421 gcgcatcgct ttcacacagt cggcaaccag ctgcgcatta cccatcagac acgcaccaaa
     6481 catgccgttc tgcacccggt cagacgggca gccgacattc aggttgatct catcatatcc
     6541 gcgcgcttct gccagctttg cacactgcgc cagcgccgcc ggatcgctac cgcccagttg
     6601 caacgctacc ggatgttctt cttcactgta cgccagataa tcacctttac cgtgaataat
     6661 cgcccctgtg gtcaccattt cggtatacag caacgtattg cgggaaagca gacgcaagaa
     6721 atagcggcaa tgtctgtccg tccagtcgag cattggtgca acgctaaacc gaccactcca
     6781 gtgaacgtca gttttttcag gcattgcgct ggtttggttg attttttgca tttcagaatt
     6841 accgtgcatt ttcaaatgta gagattattt tatcgatata tcatggggtt atgttattca
     6901 gcatcactat tcaggaggct caatagcggg gtactatacc ataacaacag gaagcgcctg
     6961 tctcattgca aaagaaaatt gagatcctct caaggcatga agctctcacg aagtgatgga
     7021 aataatctta ttagccgtta actttgttaa ggccaatgat aaacaatcca ggttcgacga
     7081 taaataaaaa atcacacatt aaactctggt gatatatctc cctgctaata gcattgatag
     7141 agaaaaaaga acccaataaa tattgggtcc ttttatataa tgccttccat actatcgaag
     7201 aacttcacat attatttctc cgatttaacc ccgaacaaat cataaattaa tttagaagtg
     7261 tctgtaagta ttttaatctc ttcctttgag gttgggtcaa acgacttcgc gaagctaatt
     7321 aattgtggtg cagcatctct cattttgctt aaaatatcag gatcgagcgt tccttcattc
     7381 accagatgtg acatcttatc cagatagcca tcaaaattca tttctccccc atcagccaga
     7441 cgcttcatct ctcccaggta cttcttcata tcacgttgag ataatttttc aaactgagct
     7501 ttcaggtagc tctcattatt ttcattaata tacactgttt ctgataaatc tgcgtaggca
     7561 ctagaatatg aaagtgaaat taacagcgaa gcaaataatt ttaagctgtt gtcatcatgt
     7621 tcctggcagg cattaacaaa cgttaaaaaa ccagaaccta ttgcttgaaa atgtatattc
     7681 gctaaaggtt cattatcctt agattcctca taaaatagct gaaccgtgga gggaaggcca
     7741 ttcttagttt ctacatcaaa agtacattgt tcaatgggag tcgcatcttt attatcccca
     7801 ggattacata tactattaat cgcgaaatga aaaatagccc ggtcaacaac tgatgcaata
     7861 aagtcacgat cgtttaaatc ctcatcagtg caatcgtcaa tatacttatt gacccacatt
     7921 ttgagatcat ttatttgctc aattaaaaac acattttttt acgtgcatgc cataaattat
     7981 gcgtgattcg ttgaataaac aaatctattt ttgcaataat tatatcccga tgacgcagcg
     8041 ctgcata
//
LOCUS       DAZGCS010000168         8005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGCS010000168 DAZGCS010000000
VERSION     DAZGCS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.171"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHK01_004559"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHK01_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO8099373.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHK01_004560"
     CDS             complement(399..1022)
                     /locus_tag="ACHK01_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099374.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHK01_004561"
     CDS             complement(1025..1306)
                     /locus_tag="ACHK01_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO8099375.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHK01_004562"
     CDS             complement(1299..1625)
                     /locus_tag="ACHK01_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO8099376.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3692)
                     /locus_tag="ACHK01_004563"
                     /pseudo
     CDS             complement(1713..3692)
                     /locus_tag="ACHK01_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
     gene            complement(3682..5184)
                     /locus_tag="ACHK01_004564"
     CDS             complement(3682..5184)
                     /locus_tag="ACHK01_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8099377.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTEQQRQQATDSQHEE"
     gene            complement(5184..5396)
                     /locus_tag="ACHK01_004565"
     CDS             complement(5184..5396)
                     /locus_tag="ACHK01_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099378.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..7516)
                     /locus_tag="ACHK01_004566"
     CDS             complement(5393..7516)
                     /locus_tag="ACHK01_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8099379.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(7513..7989)
                     /locus_tag="ACHK01_004567"
     CDS             complement(7513..7989)
                     /locus_tag="ACHK01_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO8099380.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         1716 a         2518 c         1917 g         1854 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctacaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagtcgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccaccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgttctgc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagg gcattcatat cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ctgcctcacc ctgactaccc
     3661 gccagcatcc tgaaccagga tttattcttc atgctggctg tcggtggcct gctgacgctg
     3721 ttgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccacca gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgctc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccgcagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaaa tgacgcaatc cgggagtttc
     6541 gtggcgttcc ggtgatactg ccgtccgccg ccgcccgttc accttcacga agccagatcc
     6601 cctggttatt cagttcgcgt ttctgctcag gggcaatcag cccgcgacaa tgcggacaca
     6661 tcagacgggc agcctgaccg gcagccacaa aatccgggtt attccggtat ccggtcatgt
     6721 tatccatcac cggctgaaaa tattccccgc agtgcggaca cggccagtac caccggcggc
     6781 ggtctccccg gttatacagt gacaggatac ccgttgttgg cggtgcctca tgtgcgccac
     6841 cacaacgcca tttggtatcg gtgatatccc gccccggtga actctcgacc agggtcatcc
     6901 ccgaggacat aaaggtggtg gtacgctttg aggccagcgt gaaggcatcc ccttccccgt
     6961 ccacgttttc agggaaacgg tcataatccg tcagcgccac acgacggtaa tccgaagagg
     7021 aaaagacggt gatcgacggc cagccaatct tcaggaagga gccgtcaaga aacattttat
     7081 cgtggacgtt gttgtcatta cgggaaggac tgaggcgctt gctgacctcc ggactgtggc
     7141 gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc cttcgtctca gtcatctgca
     7201 ccaccagcat atccgccgga tcacagatga tgccgtacac aatccagcca tcaatcagcc
     7261 cttcggtttt cccggttcgc gcaggtccca caaacaccac cgcgtcatat tcacgggctg
     7321 ataatgtatt aatggggtca atcatatagg gcgtcagcga tgactcccac ggaccggaag
     7381 tattggctcc ccgtggaacc cgcatataac gcctgatggc ttccgctact ggtaaccggc
     7441 caggtgggcg aaacagcgag gccacttcgc gccagatatc ggatgcgcgg ctatggctct
     7501 cgttcacctg attcacatat cggcctcatc acaacagtca atgactgcct tttccagtgt
     7561 gtcgcggatc tcatcaacca caatctgtac ttcattcagt tgtgatgcgg tccaccccct
     7621 gtccctctcc agccggtcag gccaggtttc cagtacctga actatcgctt tcaccacgac
     7681 agaaaaggac cgcctgacat cactgactgg cacaagctga ccagtttcct gctcaaattt
     7741 cagtcggtca cgttctgact ggtaccatgc tttacgcgca tgaggatcca tttcctcgtt
     7801 atctacaggc agaggagctt tcatcagctc ggcaaggata tctgtcagtc ggtacagttt
     7861 gagattgctc tcatgaccac cggctgggct tatgtttttt acccgagccg caacagtctg
     7921 tcgatgagca ccggataatg cggccagttg ggaaatattc agatgcagat tttttaattc
     7981 acgatccata actccccctg aaaat
//
LOCUS       DAZGCS010000169         7943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGCS010000169 DAZGCS010000000
VERSION     DAZGCS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.172"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..1015)
                     /gene="pyrD"
                     /locus_tag="ACHK01_004568"
     CDS             complement(5..1015)
                     /gene="pyrD"
                     /locus_tag="ACHK01_004568"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO8099381.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1126..1863)
                     /gene="ycbF"
                     /locus_tag="ACHK01_004569"
     CDS             complement(1126..1863)
                     /gene="ycbF"
                     /locus_tag="ACHK01_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8099382.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1829..2344)
                     /gene="ycbV"
                     /locus_tag="ACHK01_004570"
     CDS             complement(1829..2344)
                     /gene="ycbV"
                     /locus_tag="ACHK01_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099383.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2352..2894)
                     /gene="ycbU"
                     /locus_tag="ACHK01_004571"
     CDS             complement(2352..2894)
                     /gene="ycbU"
                     /locus_tag="ACHK01_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099384.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2906..3976)
                     /gene="elfG"
                     /locus_tag="ACHK01_004572"
     CDS             complement(2906..3976)
                     /gene="elfG"
                     /locus_tag="ACHK01_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099385.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3967..6567)
                     /gene="elfC"
                     /locus_tag="ACHK01_004573"
     CDS             complement(3967..6567)
                     /gene="elfC"
                     /locus_tag="ACHK01_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO8099386.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6592..7293)
                     /gene="elfD"
                     /locus_tag="ACHK01_004574"
     CDS             complement(6592..7293)
                     /gene="elfD"
                     /locus_tag="ACHK01_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8099387.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7376..7918)
                     /locus_tag="ACHK01_004575"
     CDS             complement(7376..7918)
                     /locus_tag="ACHK01_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8099388.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         2037 a         2015 c         1757 g         2134 t
ORIGIN      
        1 ataattagat atgggtaacg atttctttaa tcagcggcgg acctttaaaa ataaaaccag
       61 aataaatttg caccagtgag gcacccgcag caatcttttc acgcgcagcg ataaccgagt
      121 cgatgccgcc aacaccgatg atcggtaagc gaccgtttaa ttccagtgac aagcggcgaa
      181 taatttcggt gctttttaac tgaagcggac gaccacttaa gccaccggtt tgatcgcaat
      241 ttttcattcc ctgaacaaga gaacgatcga gtgtggtatt ggttgcaata acgccatcaa
      301 tattatggcg aactaaacta tcggcaacct ggatcaattc ttcttcagaa agatccggcg
      361 cgatcttcac tgcgatcggc acatatttat ggtgcatcgc ttgcaaatca ttttgcttat
      421 ttttaatcgc ggttaagaga tcatccagcg cttcaccata ttgcagcgtg cgtaatcctg
      481 gggtattcgg cgatgaaata ttgatggcga tatatcccgc ataggcatag attttttcca
      541 tacaaatcag atagtcatct ttgccctgct ccactggcgt atctttattt ttgccgatgt
      601 taatacccag gacgccgtca taatgggcct tttttacgtt ctctacgagg ttatcaacgc
      661 caagattatt aaagcccata cggttgatca aaccttcggc atctaccaga cgaaagagac
      721 gcggcttgtc attacctggc tgtggacgtg gcgtgacggt accgatctcg atcgatccaa
      781 atcccatcgc gcctaacgcg tcaatgcact ccccgtcttt atcaagaccg gctgccagac
      841 caagcggatt tttaaacgtc aggcccatgc agttaacagg tttcgcaggc actttctgcc
      901 gcaccagtgc ttcaaacggc gttcctgtaa tacggcgtaa ttgctgaaaa gtaaactcat
      961 gagcgcgctc tggatcgagc tggaaaaggg ctttacgaac gaaggggtag tacatgaact
     1021 ctcctggatt cccggtgtgc aaaccggggg cgtattatgt gcgagttcgg accaaaaggg
     1081 aattgacctg cggcaaaaaa aacgcaaacg ttttccgaac ctgtttcagg gcagattttg
     1141 ctccttcttt gcactcaacc cgccgtaatc atttatcgcc cgccaggcga catgacacgg
     1201 tgcgattgcc tgacaccagc ttgtctgacg ctggctttta ggcggcacaa ctcccgcatt
     1261 acttaacgct ttaccattta cgctcaactg aatcaggtta atgtagtaag gcgttgggtt
     1321 agtgagttgt acaccctgcg aattccgtgt ccagcgtaat tgttgataag cttccagcgg
     1381 gtcgcccggc aaaccttcgg gtcgccagaa cagtttaaat accgagcgca tcgccacttt
     1441 tacgctctga ttttcaactt tgccggatgg cacgctggca atgcttaact caaatagcgt
     1501 ttcgcgatcc acaggcaaga tgtcgctttc cgttctcagc aagcgcaacg tgcctgtagt
     1561 accgggcttc aggagaataa gtggcggagc ggccagtaat ggtgctggca ctgtcgacgc
     1621 ttcccccccg gcccaacgcg ttgggcggtt gattttactg ttaataagcc aggattcctg
     1681 actggtatta gtcagtaaaa tagatatcga ttctctgtct gccggaaaga taaatcgcgt
     1741 tccgccgacc accactcctg cccgcaattc acccgccacc agcagcgata aaacggcgaa
     1801 aatcaagagc gccaatctgt tccacgtatt agtcataatg aagtacaaat gtcgcgctgg
     1861 cattcacttt acccgactgc acatcgctat tgacaggtcg caattgcgca taaaaagtta
     1921 atggcacctt accgctatgt aggctgtagt ctttactcgc ctggttaagc ggaatcaata
     1981 tcttcgcatc atccagaatc gcaattgcca gcccgctggc tgcatccgta ccactttcaa
     2041 tggacagcat ttcgctctgc actccgtcgg gggttcctga aaaggtgacg tcaaccgtgc
     2101 tgacatctgt tccgcaatcc tgcaaattaa tgatgaaaga tttctccggg ccataattcc
     2161 catgaaaaaa acgtgtcagc gagacatcgc ctaactgcac attgatttgc ttgctatcct
     2221 gtgccagcac gcatgtatta cggcgaaacg atccgctaat gttgactgtg ccatcatggg
     2281 caaatagctc acagcccccc gttaatccca gtaagaacag aatccagatt atccgtttca
     2341 acatcactcc ttcactggta aaccagatca aaatagagaa ccgccgtcgc attaccgccc
     2401 gttgctcccg cctttgtgga cttataacga agttgatatt tgagtgtgtt atccccggct
     2461 tttaagggcg taagaggctg gacctgattg agcgggattt cttgctgact ttgcgcatca
     2521 agaatttgca cggcaatccc cgttgccata ccaccgcttc cgcctgtgtc agttagtttg
     2581 agcagtgtcg gcgcaagggt gtcagcctcg ccgctaaaaa ggacgtccgc gccagtaata
     2641 ccagcggcac aaccggtgat atcgatattc aaatcagcag cagtgcttac ggtattcgcg
     2701 gcctgaaaat cactgatatt aaaatcacca atatgaatat tttgtaatgc acttttggtg
     2761 gcgacatcac agccctgatc gaccacattc ccataaatat gcatactaac ttgttcaggc
     2821 ccagccatcc cgacatggat gttaagaatg ctaaagaaca agataacgca ctgaaatatc
     2881 gtttttttct tcatacgttc acgcgttaat caaaatttat tctcaccgtt gccgtggctt
     2941 caaatcgccc ggtttctggt ggtacacctg tcgtgctgac tggatagaca ttcattcgcg
     3001 cgatactgtc tgcatcatca ggaatagaga gcggtaatgt tccaccagca ggggaaataa
     3061 tgttgttctg gctatcggtc accaccacgc ccacgccagg acgtgacgtc ttaatcgcct
     3121 ggggatagct ggggtctgtg gttgccgtca gactcaggtt gacgttaact aaccccgcgt
     3181 ttcccgtgtt acacctgact ggaattgcaa gttctgccgt tctggcccca agaggcttat
     3241 gccctacaac acgaaagtta gccgcaaata cgtcgcccag atcgacctcg attgtttctc
     3301 cggcattgac ggaacatgag ccaagtgaat tgatagtacc agacaggtaa tacactaaaa
     3361 ccggtgtacc tgtagtcgtg caggaatcac ctgcgggtat gttgtagcat tcaaataatc
     3421 gcgccagcgc cataggagga atgacgaccg aaccaacgaa cggctgcaat attttcaggg
     3481 aaaagctacc gctattaccg ctagccccct ggccacctaa acgttgaccg ccgggatcgc
     3541 aaatatgcca tcgtgtatct gcccctgtgc ccacgttttc gaaaggaacc gcagtaggag
     3601 aactgccttt aacctgcaat aaaactttgg cggcaagata gtcattaatt ttgtaccagt
     3661 tgtcgcctaa atagactaac ggtgaatccg cggcgaatgc ccatcggcca cttctgacat
     3721 tgtctttatc gcaatcacag gaaacataat aatcattcga ttcgttccac tgataaaatt
     3781 tgtctttaac tactccagcc gtattttctt cttcctccag ggttttaacg ccaaaattga
     3841 cgccaatata cggattcccc tgagtggtat gacactgccc gctataagca gcagtttgag
     3901 cgcaccatag cattaagacg gcggcaaaaa atagtcgtag taataacacg catttttctc
     3961 caaagattat ctgcatatgg catccattcg cgtaatcgca gagttaattt gttgttcggt
     4021 aagctggtaa ctgatatgac aaatttgatc gggagcttcg ccccagcgta cagttatcgc
     4081 tccctgagcg ggcaaaccgg acatatagag cgagccatca tcatcaataa tgccgctatt
     4141 agtctgtatg ccgtccagcg tcgctatcgc gccaaaacgc agcggtatac cctgctttga
     4201 tgttttcatt aatacccttg ctcccgcatg ggcattgaac tccgccagaa ccaacgcccc
     4261 tttggtggga accacgttga ctactgcatt ttcaagatcc acgttacgtt ttaacgatgc
     4321 cgcatcaagt gcgattcgat tttcccgata agaagtggca taggggacaa cggtatatcc
     4381 gcgccagtca gttttcagcc cggtgctgtt cgccacacga gtattctctg cgcctggtgc
     4441 tgcaatcagg acgttagttt cccccagcga ttgccccagg gtaatgccct gtgaatgggc
     4501 aactaaactg cctgaaagag cgtagttcag ttgttgttga ctgccgttat cgctgtagtt
     4561 gtagcccact cgggcatctg caaacgcccc tttgtagtcc atgctggcgc taccattagc
     4621 cgttttcccc tcgctgttat atccctgttg cacgctgtag ctcaggttac cgtcatcgag
     4681 tagtgtctcc gttaaaccca ggttttgtgt aatgtgccct tcgttatcga tactggtgtt
     4741 agaggtcatc caggcgtaat tttttttcct ggtatagcta tcattacttc tggggagcaa
     4801 cagattgagc ggcagggaaa aatttagtgc aaacacctga tcagcatcag gttgaccacg
     4861 ggacttactg tagttccagg aaacccccag cgagagatct ttaatttgcg tgttgtagcc
     4921 aaactgtaat agccgatcct gttgatcggt atgccagtaa gtttgctggc taccacttaa
     4981 ataaaacgaa ccgtactcgc ctaattgctg ggagatattg acctgcagtt tgccacgttt
     5041 ggtgtaaaac aaattgtaat aacgcgacca catcggcgta tcttcatcat caccgtcatt
     5101 aaattcgtag ccatccatat gtttatacat ggtgtcggaa agggtgtaga aacccgacgt
     5161 cgaatagcga tagcccatca attgaaaatt agtgcccaat tggttgagcg ttttggcata
     5221 cagaaaacga tatgactgac ctttatgttc gctttggtct gccagcgtac tcttcgcctg
     5281 ggtcacatcg aacgaaattg ctccgaaatc gccaaggtta aatcccagac caaacatggc
     5341 ggcacggtaa tattcagcat attgtccacc accataccat gtcgttcccc acggtccgcc
     5401 ccactgcaac gtggcctggg caaatttgct ctcctgctgt tcattactat tggttctgta
     5461 tttcgccagt gtcaccgcgt atttgattcg cccctgacgc tggagtaatg gcacgctgga
     5521 aaacggtacg ctgtagctat tgacgctgcc gtccgcttct ttgatttcaa ctaacaaatc
     5581 accgctcgac gacgtggaat agagatcact aatttcaaaa gcaccaggcg atacataagt
     5641 ttggtaaatg gtgtaaccat tttgcttaat cgttatctgg gcattgcttt tggcgatacc
     5701 acgcaccaca ggcgcaaacc ctttcaggct gtctggcagc atgttgtcat ctgacgccag
     5761 ctgcacacca cgaaagctga cactatcgaa aaaatcgcct gcagtataat catcacctac
     5821 ggtcagttcg ccttttagtg gaataaccgc ccgctgcaaa tagctgctga gattcttcca
     5881 ttctgcggtt tggccactac tgcgactcca tgttgaattg ttgcgcaatc tccatgggcc
     5941 taaattaacg ccactgttta aattcagaaa atagctgtcg ccagaatcac tgtctgcgct
     6001 gctatgaata ctgttagccc cgctaaacga atatcccagt aatagcgcgt tgatcccttc
     6061 atcccaacgt tcagggggga cataaccacg ggcattactt ttgatggcga tttgcggtac
     6121 tgaaatttcc agacggagtt tattcacatt aaacgttacg ctggcatccg gaataatctc
     6181 cgccaatggc acacacgctt gtttgtccag ttgtttaaaa gccggaaacg catcagtatt
     6241 aacacccatt gcatccaggc tttcagtcgt caaacaggcg gcaatcccct tctctggcgt
     6301 tatctcaaca aaattgacgt tgcgcgtatc tacaattgtc tgattcagaa caatatcgac
     6361 acgataaacc cctggcgtta ttttttgtcc tttttcgaaa cgagacaaat cggccaccat
     6421 tgagggatca tctttgaaaa aagcaggatc gaaccaggtt tcagcttgtg cattgaacgc
     6481 tgccgacaca aacaccacca gcccaccaat aaacgatggc actccaccag agcttaacag
     6541 gctgtgttgt cgatgagttc tatacatctc gctctcttaa ctgaagacgg cttacgattt
     6601 acagttgagt tgcggcgttt ccgccccgta gtcattgatg gttcgatacg ttacgctgct
     6661 atttttggcc ccctttggca gagaaaattg gtatttatca aagggggaaa tcatttgatt
     6721 tttaactact tttccaccta ttttcaggtc tgtcagggta atgaaataag gtgttgggtt
     6781 actgaccgta accatgtcag ctgacgaagt gcaacgcagt ttcgccggag cctcgcctaa
     6841 gggatacgct aaaccttttg gtcgccagaa aagtttgatg acgctttgtg tggcaatctt
     6901 cagggtattt ccctcttccc gcttcgttgt tgaagggact gcccgtacgc tagtgaagaa
     6961 aagggtttct ctgtctttcg ccaacggcgc accaatgtac ataatgcgta acagattctc
     7021 attagccgga ttcagcacaa atagcggtgg tgtaataata aaatctgctg attttttatt
     7081 atctggggtc atcacccatg actgaataag ataaggaaca tccggactgg tgttacgtac
     7141 acccagtgaa atttgctctt caccttctga ataaacaaga cgtgttgctg caagtccaat
     7201 cccgccttta cctgccgccc atgcggatga gcagctgagt aatattgccg ttaaacttac
     7261 ggtcacaatt ccctttgtta tgcaagtttt cataaatagt cactcttaaa aaatatgaat
     7321 atcccctcca gaacactaga ggggtactaa ttaataaaac aattaatgag ttctattaat
     7381 aatagttaat atggaaattt gttaccgcat tcacattacc agaagtcgct ttccccgtag
     7441 cgatataatc aactttaaag gtcactgttt gctcagcaga atccgctaac tgaatattcg
     7501 acgtttcggt accgggagtt accgatgtag aaccatctgg cagatacatt tgtagagcaa
     7561 cattcgtcgc tgcatcggtg gtagcctggt tggcaaatgc tgtaggttgc acgccgtcag
     7621 ccgtaccact aaaggtaaac gtcgcatttt tagttacagt aacatcacag ccttctaagg
     7681 caattggtac atcagtttta acgccagaga ctgctccatt agtttgcaac ttggtggcgc
     7741 tgacatttgg caatgtgact actgaatctt tcgtcgcagc taccagggta caagctggag
     7801 caataacttt accattaaat gttactttgc catccgtgat agcattatca tcagctgcca
     7861 taacgctgga cgttgcacat accacagtta taaacgccgt caatacactt tttttcatcg
     7921 tcatcatcct gatctcttcg taa
//
LOCUS       DAZGCS010000170         7889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGCS010000170 DAZGCS010000000
VERSION     DAZGCS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.173"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..243
                     /locus_tag="ACHK01_004576"
     CDS             <1..243
                     /locus_tag="ACHK01_004576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099389.1"
                     /translation="FLSVHITSIFECKKPTASMLWALFFGVLMSEGTKKKTRSFYAAG
                     LLFTSTGNRVPFRPLPWQPIDGEVAFPVAVSLLLTL"
     gene            complement(226..366)
                     /locus_tag="ACHK01_004577"
     CDS             complement(226..366)
                     /locus_tag="ACHK01_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8099390.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(558..818)
                     /locus_tag="ACHK01_004578"
     CDS             complement(558..818)
                     /locus_tag="ACHK01_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8099391.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHDLPASHRGNNQMSLPLSQN"
     gene            complement(859..1968)
                     /locus_tag="ACHK01_004579"
     CDS             complement(859..1968)
                     /locus_tag="ACHK01_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO8099392.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            2126..3310
                     /locus_tag="ACHK01_004580"
     CDS             2126..3310
                     /locus_tag="ACHK01_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO8099393.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGRTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            3310..3822
                     /locus_tag="ACHK01_004581"
     CDS             3310..3822
                     /locus_tag="ACHK01_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8099394.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3944..4252
                     /locus_tag="ACHK01_004582"
     CDS             3944..4252
                     /locus_tag="ACHK01_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099395.1"
                     /translation="MAVERGVKLSRPIERGAEKITYVEITGAIEQAGSLRGLSLSDVL
                     NLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLCAVAVNFMSEPDSGAKSVQETAA
                     "
     gene            4261..4416
                     /locus_tag="ACHK01_004583"
     CDS             4261..4416
                     /locus_tag="ACHK01_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO8099396.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            4403..7210
                     /locus_tag="ACHK01_004584"
     CDS             4403..7210
                     /locus_tag="ACHK01_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8099397.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     QARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRLLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            7223..7711
                     /locus_tag="ACHK01_004585"
     CDS             7223..7711
                     /locus_tag="ACHK01_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099398.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
BASE COUNT         1769 a         1967 c         2263 g         1890 t
ORIGIN      
        1 tttttaagtg tccatataac gtccatcttt gaatgcaaaa agcccacagc ttctatgctg
       61 tgggctttgt tttttggtgt cctcatgagt gagggaacca aaaagaaaac ccgcagtttt
      121 tacgctgcgg gcttgttgtt cacgtcaact ggaaataggg tgccttttcg acctttaccc
      181 tggcaaccga ttgacgggga agttgccttc cccgtcgcgg tttccttact gcttacactg
      241 taagaacgcc gcaaactccg ctccccagaa actcatccgt atttcacaca gcgaaccgtg
      301 cagcatccag atgatgagga ttgccgtcac gcagaatgtg atggccgtaa gcgatttttg
      361 cgacatagcg cttgctcctt tttcggggag gcgctaacct atcacttgct gagggtagat
      421 ggttagggcc tcgggttaac gttaaagttg actcggggcc tttccacatc tggccttcgg
      481 gtattccctc cgaccatcag ccgaaaggca cccgcacgta atctatcgct tttttgttac
      541 tccggcaatt ctgcctgtta attctgagat aaaggcaaac tcatctgatt gtttcccctg
      601 tgtgaagctg gcagatcatg ccacgggata ccttctgaag agtgaacgcc ggaggcgtgt
      661 tttgatgtga atttatggaa agcttccagt gttgagaagc atacgccgca ttccagattg
      721 ttacactggt aatacttttg ccgcacggtg tttgaatcat tttccggacg actggtgcgg
      781 atacgggcag atgcgccaca aagcggacaa cggaacatag cgacctccct taacgtggtg
      841 ctgcctattc taagttgctc actctgtttc cgctatccat tccgggattt ttgcctcaag
      901 ctcaagctgc gtggtaaaac cgctgttatc aatggtgtgc tcggcttttg caatgatcca
      961 gtcctgatta tcaatctcgc ttttaaatcc tgttaccgtg ccatgcattt cggggtagag
     1021 ttctgcacgt ccacgcgcca gcgtgatgga gaatgatgcg gctccgcgtt gtagttgctg
     1081 ccactttgcc gccgctgcgc gtcttgctgc ctgctcgttc tgataagtct tgcgtaacac
     1141 aaacacattg ccttccgcgc cttccatata atcaccttcg cggctgctgc ttttctcctt
     1201 tttgggtttt ggcggtttac ggcgtttcac gctgactttt ttctttttcc cgtaattaag
     1261 atcaagccag taagcgcgta cccccgtata cgcttcgcgg tcagcaatgc ggaactgatg
     1321 gcgatcgccg ctgctgcgtg tgatggcaaa cgatggcaac ggctggccct gtgcgttcac
     1381 gccaccgccg ggcatgatga ataacagatt accgcttttt accgtggtga ttgcgcccag
     1441 catttccgcc atgcgcgtaa ggaaggacat gtcgctttct tcggtctggt cggcgtggtc
     1501 gatttcgata tccatcagca tttcgctgat ttgcggtttc agaccatacc gatgagcgat
     1561 ggcggatacc acacgctcaa cggtcacatc atgccaggac acctcacgtt taacgttaaa
     1621 ttcatcccga aaatctgcgc ttctggctga aacagtcagc ctgtccggcg gtccttcgtg
     1681 agcgatttca tcaacaatgt aagtgccttt ttctgtcagc ggttctcctt tccagccaat
     1741 gagaaccgtc aggcgcgcgc cccgtggcgg tagctgcaac tgaccatccg catcatccag
     1801 cgtgatggtg agctggtccg cctcaaatcc ccggttgtcg gtcagtgaca ggctcatcag
     1861 gcgctctgcc acgcctgaca gcgttttacc ctccgcgaga atatcaaaat ccggcatttt
     1921 cacggggtct gtgccctgac tgagcaattg catggtggtg tcggtcatct gctccctccc
     1981 tgtgcggcat ggtcgcatgt gcgtgcggag ggggttactg ctttttgttg tcgccgtggc
     2041 gggagaacgg cgcaggggtg agattacgcg cgtggtgggt gatgattgtt gccgaatcat
     2101 ttaacggata caaggggctg aagctatgag tgaaactcgt tttcatggtg cccgtgtcac
     2161 ggaaagtacc gacctggtaa cagcgattaa tgatgttgat tccagcgtta tcggtatcgt
     2221 ggcaacggcg gatgatgcgg atgcggagct gttcccgctg aacaagccca cactgctgac
     2281 ccgcgtcaat gacgtgctgg gaaaatgcgg gactacgggg acgctttatc gtgcgcttaa
     2341 ggctatcgca gaccaggtga gcacaaaagt gatcgtcgtt cgcgtggctg aacacaaaga
     2401 agaaggcggt aggacgcagg atcaactggt tatcggtggt tctgaatctg acggcagcta
     2461 tacggggatg tatgcgctgc ttgttgcaga gcaggatgaa agcatcggat accgtccgcg
     2521 tattctggcc gcgccggagc tggacacgga ggcggtgaca aaatccctgt gcgtgattgc
     2581 gggtaaactg cgcgcgtttg tgtatgccac atgtcatggt tgtaacacga tggctgaggc
     2641 gattacctac cgccagaaat tcaacgaacg tgaggtgatg ctcttatggc ctgacttcat
     2701 cgcctacaac ccgaaaagtg gcaaaaacga aacgttcccc gcgcctgctt atgcgtgcgg
     2761 ccttcgtgcg tacattgacc atgagcaggg ctggcacaaa tcgctgtcca acgttccggt
     2821 taaaaatgtg ctggggatgt cgaggcatgt gttctggtcg ttacaggccg aagacagtga
     2881 tgccaacagc ctcaacaaca aagaaatcac gaccattatt cgtcgtaacg ggttccgctt
     2941 ctggggcaac cgcacaccgg aaacgaacgc ctacatcttt gaggtgtata cccgaaccgc
     3001 acaggtgctg gctgattcaa ttgcggaagc gcagtttgaa accatcgaca gtccactgac
     3061 gcctgcgaac gtgaaggatg ttatcagtgc catcagggca aaattggatt cgctggttac
     3121 tgccgggaaa ctgattggcg cggagtgctg gtatgacgtg gtggataacg gcaccacgga
     3181 tttacgtcag gggcgtgtgc gtattcgcta caaatatacg cccgttcccc cgctggaaga
     3241 catggagctt taccagacgt ttactgatga attctttggt cccgcatttg cggtgctggg
     3301 aggtgcctga tggctgtacc aaaacatctt cgctttttta cgttgtttgt ggatggtgaa
     3361 aacgaagtgg gtaaagtgac atccgtcacg ccgcccaaac tgacgcgcaa aaccgacagc
     3421 tatcgtggtg gtggcatgat gggagcagta agtattgatc tcggtctgga cgactccgcg
     3481 cttgatgcga gctttgtcat ggggggagct gttcgtgcac tgttccttaa gtatggcggc
     3541 acgattgacg gcacgctgct gcgttttgcg ggtgaatatt acaccgatgc agaaagcgat
     3601 ctgtatgaaa tcgagatgcg cgggcgtgtg acggaaattg atatggggga ggccaaacag
     3661 ggcgaagcca catcacacac ttacgctgtc aaaaacacct actacaagct gagcgttaac
     3721 gatcgcccgc tgtgggaaat cgacctgttg aaccacatct accggaagga cggcaaggac
     3781 attgtgcctg accgcattcg ttccgcgctt gggcttggct gataagtaat atgcaggcgg
     3841 cgcagtgcgt cgcctctgac tgaaaaggag acaactgatg aaagatatcg atactgaaac
     3901 ccggaataac accgtagcgg atgatgtgac gtcaggtgag gatatggctg tcgaacgtgg
     3961 tgtaaaactt tcccggccaa ttgagcgtgg tgccgaaaaa atcacatatg tggagatcac
     4021 cggggctatt gagcaggcgg gatccctgcg tggtctgtcg ctgtctgatg tgctgaatct
     4081 gaaagcggat accatgttca cgctgttgcc tcgcgtgacc tcgccacgac tggatgaagt
     4141 gatgattaaa aaaatgtcgt cacgcgattt tattcagttg tgcgctgtgg ctgtaaattt
     4201 tatgagcgag ccagactctg gcgcgaagag cgtgcaggag acggcagcgt aatcaccctg
     4261 gtgtgctttg agcacatcga agatctggtg gcggatattg ccgccatttt caactggtcg
     4321 cccgccgaaa tcttcatgat gacgcccggc gaagtggtta gctggcgtga gcgggcggca
     4381 cttcgcagcg ggaatgcaga taatgaagac tcttgacatc cgggtcgctt tcagcgccgt
     4441 tgacaggctg acccggcctg ccgaaaacgc ccgccgcctg atggggcagt ttggtgactc
     4501 cattcagcga acgcaggggg cgatcaaaaa tctcgagcgt caggcgcgtt catttgagcg
     4561 cgcccgcgac gctgtcagta aagcggatgc gggtatcgtg aaagcacgac gccagcttaa
     4621 cgcccttaat cagttacaac gcacgggtac agtgctcagc gaaaaacaac aaaagctgat
     4681 gcagcagtta agcacccggc ttgaacgcct gaatgaatcg cgcacacggg aaattcagaa
     4741 aatgcgggag cttggcggag aactgaaacg ccacggcatt tccctgacag gcagcgataa
     4801 caccatccag caggccatca gacgcaccga acaatacaac aaccagcttg aacgcgaacg
     4861 gcaggcgctt gcgcgtgtaa cgcaggctcg tgagcggtat tcgcgcgcgc aggaaaccgc
     4921 gggaaaactg aaaacaggtg gtgcgctggc aattggtgcg gcagcggcgg gcggctatgc
     4981 tgccgggcgt tttttgcagc ccgcgatcgg gttcggcaaa gagatgtccc gcgttcaggc
     5041 gctgacgcga atcgacaaaa acagcccgca gtttaaggcg ctgcgtgagc aggcgttaaa
     5101 acttggctct gaaacacagt ttactgcgag tgatgccgcc agtgggcaga gctttctggc
     5161 aatggctggt tttactccgc aggccattca ggccgcattg cccggtgttc ttaatatggc
     5221 gctggcaggt ggcgtcgaac tcggcgagac ggctgatata ggctccaata tcctcacaca
     5281 gttcaacctg acagccgatc aaatggaccg ggttggcgat acgctgacag cggcattcac
     5341 ccggaccaat actgatttac gcgcgctggg cgaaaccatg aagtataccg gtccggttgc
     5401 cgcaaaactt ggtatcagtc ttgaagaagc ggcagccatg gccgggatgc ttgccaataa
     5461 tggtcttcgt ggaagcgatg ctggtacggc catgcgcgca agtctgtccc gccttgcatc
     5521 accgccaaaa gctgcggctg atgcgctgaa agagctgggg gtgtcagttg ctgacgccag
     5581 aggcaaaatg cgcccgatgg aggatgtgct gcttgatctc tataaggcga cacaaaaata
     5641 cggacaggtg gaccaggttt ccttcttcaa ggacatcgcc ggagaagagg cgttcgttgg
     5701 tttgcagacg cttgttgcgg cggctggttc aggagagctg caaaaactga ccagagaatt
     5761 gcagggggca aggggagagg ccgatcgcgt tgcaaaagta atggccgata atcttgatgg
     5821 ggacctgaaa aatctcgaca gcgcatggga aggtcttcgt attcgcatca gtgatctggt
     5881 tgacggtccg ctgcgttctg tcacgcagtg gctcacgcgg gtgcttgaaa aaatcacctc
     5941 gctggcgcag gcccatccgg tactgacgcg ccagctactg atagcaggcg gtgcgttgct
     6001 ggcaatgact gcaacggttg gctcgttgtc gctggctatt ggtgtgcttg ctggcccgct
     6061 ggcaaaactg cgtcttggct tttccctcct gaccggttca atgaatgctg tcagactcct
     6121 gccagcacta tggggaatgg tgacgggttc cgtttcgtta ctggggggcg ctatcggggc
     6181 gttgttcagt ccggtcggcc ttatcgtggc tgcgcttgcc ggagctgccg ttctcatctg
     6241 gaaatactgg gatcccatca gggcattttt tgccggggtg ttcagcggga ttatggaaag
     6301 gctgaccccg ttgcgcgaaa cctttgaacg gtttggtcct gtttttgacg caatcgggag
     6361 tgggatcagc caggtgttta actggtttaa atcgctgctg tctccgatgg agtccagcaa
     6421 ggaaactctg gataaatgta ccagtgctgg cgagatattc ggtaacgttc ttggcggtgc
     6481 gttacaactt gttctgacac ctgcaaaaat gttgctggat acgctggcgt ggatacttga
     6541 aaagcttggt gtgcttccgg atgaagcgga aagggcgcgc aagaaaatcg aagacgcaca
     6601 gcgtgcggcc attcttcagg acaaggttgc cttgcttcag ggggacctgg cgaaaatcaa
     6661 tccgccgaag cctgtggaaa atggcaatgg caccggaggt gataacccca aagacaataa
     6721 accgctcaca gacagcaata ccggtacgct gcgcagactc agcaaaattg ctgataacac
     6781 aggtaagctg gttgatgaga cgaaaaaacg cattggccct ggcgatattg tctttaagaa
     6841 cctgccccgc gcacttgctg ttcgtgggga gtggcaggag cggaagattg cgcaggtcag
     6901 taagcctgcc cccgcaatta atatcacacc cgtagtcccg gctccgctgc ctccggcgct
     6961 ggtccctgtt gttgcggcca gctcccgccc ggtggcggag gccatacgat cgccagtggc
     7021 atcagttcct gcagcttccc gtaatcggga gcctgctgtc tccagatttg gcggtgaaat
     7081 tcatgttcat ctgcataacg ttgttacgca gaatccccgc gaactggcga aactggtcgg
     7141 tgaaatggtc agggcagaaa tggaacggcg cgcccgtgcc gggcgtggca gtttttacga
     7201 taaagattga ggagtcatgg ccatgatgat gatctatggc atgtttgttt ttgagctgcg
     7261 cacgctgccg catcagcagt tacagcaaaa caaaagctgg cggcatgtga aaaatgaacg
     7321 cgttaaccgt tcagcaagct ggcagtatat cggtgcaggt gatgatcgca tcgttctttc
     7381 tggtgtgctt tatcctgaaa ttacaggtgg cgaagtgtcg ctgtctctgc tgaccacgca
     7441 ggcgtataca ggacgaccct ggcctttgat tgatggcgtc gggcagattt acggcatgta
     7501 tgtcctgact gaaacgaata cgacccgctc cgagtttgat cgctacggta aggcgaaaaa
     7561 gatagaattt tcactgactc ttgaacgctg tgatgaggat ttgcgggagc gcctgcaatc
     7621 ctcatcgttc agcgatatgt tgtccggctt caaagataag gtgacatcat cccttaatag
     7681 cgcggccagc tcagttaagg ggctgctctg atttaacgta tgtcgccaat ttcctgatga
     7741 aggtgactgg cgacttgctg ttgtatgtcc ttctcagaaa attgtttttg aataacaaat
     7801 aacaggattt tataatctct taaccttata acatgtgtgg tctgaaataa taattaagga
     7861 gattttcgtg ctgtcttact taatggcaa
//
LOCUS       DAZGCS010000171         7888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGCS010000171 DAZGCS010000000
VERSION     DAZGCS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.174"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..210)
                     /locus_tag="ACHK01_004586"
     CDS             complement(<1..210)
                     /locus_tag="ACHK01_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO8099399.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDT"
     gene            342..1625
                     /gene="ydfJ"
                     /locus_tag="ACHK01_004587"
                     /pseudo
     CDS             342..1625
                     /gene="ydfJ"
                     /locus_tag="ACHK01_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1714..3174
                     /gene="ydfI"
                     /locus_tag="ACHK01_004588"
                     /pseudo
     CDS             1714..3174
                     /gene="ydfI"
                     /locus_tag="ACHK01_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(3210..3413)
                     /gene="ydfZ"
                     /locus_tag="ACHK01_004589"
     CDS             complement(3210..3413)
                     /gene="ydfZ"
                     /locus_tag="ACHK01_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO8099400.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3591..4277)
                     /gene="rspR"
                     /locus_tag="ACHK01_004590"
                     /gene_synonym="ydfH"
     CDS             complement(3591..4277)
                     /gene="rspR"
                     /locus_tag="ACHK01_004590"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO8099401.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4366..5112)
                     /gene="ydfG"
                     /locus_tag="ACHK01_004591"
     CDS             complement(4366..5112)
                     /gene="ydfG"
                     /locus_tag="ACHK01_004591"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO8099402.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5249..7294
                     /gene="dcp"
                     /locus_tag="ACHK01_004592"
     CDS             5249..7294
                     /gene="dcp"
                     /locus_tag="ACHK01_004592"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO8099403.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            complement(7338..7856)
                     /gene="ydeJ"
                     /locus_tag="ACHK01_004593"
     CDS             complement(7338..7856)
                     /gene="ydeJ"
                     /locus_tag="ACHK01_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO8099404.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1972 a         1834 c         2004 g         2078 t
ORIGIN      
        1 ggtatctttc agcgtctcct gaacaaaagt ttttgcagaa tctttatcgg cggtgcgagt
       61 aactgaaagg ccatcgttgc tggtggattt gatgatcact tctacatcgt cataacgttt
      121 actgacacgt cgcagcattt cttgttgcag ggcaggaaca gaaccttttg gcattttgtt
      181 gattttggct gcggggtatc tgtggggcat atatgggaca taaaaaggcc tcaaataaac
      241 agcaaaatcg agattaggga tttttagaaa aaatacaacc atctgaaaaa acttagaaaa
      301 cacccaaaaa cccacagaat agtaaaaaac atctatgaat tatggatttc cagttatatt
      361 cgctcggcgc agcgttagtg tttcatgaaa tattttttcc tgaatcatca acggcaatgg
      421 cgttaactct ggcaatggga acctacggtg caggttatgt ggcgcgtatt gtcggagcat
      481 ttattttcgg caaaatgggc gacagaatag ggcgtaaaaa agtgctcttt attaccatca
      541 ccatgatggg gatctgtacc accttaattg gtgtgttacc gacctatgca cagattggtg
      601 tttttgcacc catcttgctg gtgacgctgc gtattattca ggggttgggt gcaggtgcgg
      661 aaatttccgg tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta
      721 tctcctcatt tgtagctatg ggaactaact gcggaacctt gagcgcaacg gcaatctggg
      781 cctttatgtt cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt
      841 tcctggcgag cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa
      901 gcccggtctt tgagaaggtt aatgacagca accaaccgac agcaaaacct gcacctgctg
      961 gtagcatgtt ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggctg
     1021 gtaactcagg tttaattcag actttccttg caggctattt agtgcagacg ttattgttta
     1081 acaaagcaat tccaacagat gcattgatga ttagttcgat tctcggcttt atgaccattc
     1141 cgttccttgg ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata
     1201 cctccgcgat tgtgctggca tggccaatgc tttctatcat tgtagataaa agctatgccc
     1261 cgagcaccat tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg
     1321 ctctggaaaa cattaccatg gcagaaatgt tcggctgtaa aaaccgcttt acccggatgg
     1381 ctatttctaa agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta
     1441 ttttctgcac catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag
     1501 tgattggttt aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc
     1561 tggaagacgc tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc
     1621 tgtaaatcct caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat
     1681 gtagcttgct atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa
     1741 gcgacactcc ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt
     1801 ggtgcatttc accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc
     1861 agtgactggg gatattatta ggtcaactta atcggcggcg aacagcaaat tgccgattta
     1921 caacagcaag ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc
     1981 gtcgttggcg tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg
     2041 gctgcgatgt gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat
     2101 ttccactctc cggcgaccgg acagttaatg ctcgatcacc cgatggtcgc tgccgacgtg
     2161 caaaatcccc accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt
     2221 aaagcggcag gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt
     2281 catgttatgc gtgatgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa
     2341 tggatcgaag ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg
     2401 acagaggata cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgcgggcgtt
     2461 gcctgtgaac ctttccgcca gtgggtaata gaagataact ttgttgccgg acgtccggaa
     2521 tgggaaaaag cgggagccga actggttagc gatgtgctgc cttatgaaga gatgaagttg
     2581 cgcatgctca acggcagtca ttcattcctg gcgtatctgg ggtatcttgc cggatatcag
     2641 cacattaatg actgcatgga agatgaacat tatcgtcatg cggcgtatgg cttgatgttg
     2701 caggaacaag cgccgacgct gaaagtgcag ggcgttgatt tgcaggatta cgctaaccga
     2761 ttaattgcac gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat
     2821 ggcagccaga aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac
     2881 agcaagttcg atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt
     2941 gatgaacagg gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct
     3001 gtacaaagta gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc
     3061 tttggtgatg atttgccaga cgatagcttg tttactgcaa aagtgacgga agcgtacttg
     3121 tctttattag cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag
     3181 caatgccgcc atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga
     3241 ggtgccagtt tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca
     3301 gcggtcagac cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc
     3361 ataacacggc tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt
     3421 ttctcctttc tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc
     3481 gagtgaaata ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg
     3541 taaggcgggg ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg
     3601 ctaaaccagt cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa
     3661 tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata
     3721 tcaagatgct ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc
     3781 acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca
     3841 atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg
     3901 cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg
     3961 gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg
     4021 ccgtttcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt
     4081 tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca
     4141 ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg
     4201 acaatgtcgc gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt
     4261 tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact
     4321 tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac
     4381 attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg
     4441 agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac
     4501 ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa
     4561 ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag
     4621 atccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt
     4681 accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat gaccatgatt
     4741 acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt tgttgttggt
     4801 atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc
     4861 caagccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa gcgatgccag
     4921 catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc
     4981 cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg
     5041 cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac
     5101 taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg
     5161 cttaagagcg ggtaatactg gtttgcaaga ttgcactacg ttatcagtaa gcctacgcta
     5221 tggaacacct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag
     5281 cacactgccg tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc
     5341 attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc ttaacccgca
     5401 aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat tacttacccg
     5461 cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct
     5521 tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga
     5581 attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga
     5641 atccatccgc ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc
     5701 gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca
     5761 gtttaaccag cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc
     5821 gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagtggcgg ctcgcgagaa
     5881 aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc
     5941 cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga
     6001 aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca
     6061 gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc
     6121 aaaaacacct gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg
     6181 tgctagcgat gaattagcct ccatacaggc ggttatcgat aagcagcaag gcgggtttag
     6241 cgcgcagccg tgggactggg cattttatgc cgaacaggta cggcgggaga aatttgatct
     6301 tgatgaggcg cagctcaagc catattttga attaaacacg gtgttgaatg aaggtgtatt
     6361 ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta
     6421 ccatcctgac gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt
     6481 ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt
     6541 tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtttgca attatcagaa
     6601 acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga
     6661 atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac
     6721 caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca
     6781 tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga
     6841 actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact
     6901 gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca
     6961 ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat
     7021 accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg
     7081 ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga
     7141 gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg
     7201 taacagcgaa gatctggaac gcctgtatcg acaatggcgc ggtaaggcac ctaagattat
     7261 gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctgagata
     7321 tcgccaggcc agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc
     7381 gctaaagcta ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg
     7441 cctttaatat gccacgcaaa ccagacggta cccgctggcg taccatcttc accgccctcc
     7501 ggtccgccgt agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcaccg
     7561 gttgccattt ctgctgccac tttctcactc accgcagaat atcgttcaag agattgctgg
     7621 cttacgctga ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa
     7681 aatttgggtg tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat
     7741 tcggcggtag ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg
     7801 ttttcaatag tatcggtatc tacaagttgt acaattttat ctctattaat gttcactgtt
     7861 ttttgtccac gcttttatat gtaaaggt
//
LOCUS       DAZGCS010000172         7757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGCS010000172 DAZGCS010000000
VERSION     DAZGCS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.175"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHK01_004594"
     CDS             complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHK01_004594"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO8099405.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHK01_004595"
     CDS             complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHK01_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO8099406.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHK01_004596"
     CDS             3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHK01_004596"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO8099407.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4937..5551
                     /locus_tag="ACHK01_004597"
     CDS             4937..5551
                     /locus_tag="ACHK01_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO8099408.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5590..7041
                     /gene="trkH"
                     /locus_tag="ACHK01_004598"
     CDS             5590..7041
                     /gene="trkH"
                     /locus_tag="ACHK01_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO8099409.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            7053..7598
                     /gene="hemG"
                     /locus_tag="ACHK01_004599"
     CDS             7053..7598
                     /gene="hemG"
                     /locus_tag="ACHK01_004599"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO8099410.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1725 a         2097 c         2110 g         1825 t
ORIGIN      
        1 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
       61 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
      121 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
      181 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
      241 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
      301 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
      361 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
      421 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
      481 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
      541 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
      601 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
      661 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
      721 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
      781 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtggctc
      841 attggcacat ggcccatatg ctccacgccg ccgacaagac atgcctgcgc atcgccagtc
      901 atgatcattc gtgctgcgtc atgcaatgcc tgcatggatg aaccacacaa gcgattgacg
      961 gtcaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatatta
     1021 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
     1081 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
     1141 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
     1201 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
     1261 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
     1321 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
     1381 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
     1441 accagcgcca tatccgcttc cgccggagtg gcgataatgc cttcctccag acagcgcacc
     1501 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
     1561 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
     1621 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
     1681 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
     1741 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacgac atccagcaga
     1801 tatgccggac ccataggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
     1861 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
     1921 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
     1981 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
     2041 ggcatccggt gtaccgggtt aaaaaagtgc attccgcaga agttttccgg gcgttccagc
     2101 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatccggg
     2161 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcgacaacc
     2221 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
     2281 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
     2341 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
     2401 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
     2461 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
     2521 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
     2581 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
     2641 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
     2701 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
     2761 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
     2821 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
     2881 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
     2941 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
     3001 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
     3061 tcgcagccac cgcccagcgc atagccgttg gcggcagcaa tggtcggcac cggcagatct
     3121 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
     3181 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
     3241 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
     3301 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
     3361 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
     3421 gtctcctgaa tccgcaaggt gatctggtcg taccagatga gtcgaagtgt gtattttgtg
     3481 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
     3541 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
     3601 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
     3661 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
     3721 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
     3781 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
     3841 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
     3901 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
     3961 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
     4021 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
     4081 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
     4141 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
     4201 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
     4261 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
     4321 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
     4381 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
     4441 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
     4501 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
     4561 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
     4621 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
     4681 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
     4741 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
     4801 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
     4861 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
     4921 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
     4981 agagatcaaa aaaagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
     5041 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
     5101 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
     5161 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
     5221 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
     5281 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
     5341 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
     5401 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
     5461 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
     5521 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
     5581 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
     5641 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
     5701 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
     5761 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
     5821 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
     5881 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
     5941 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
     6001 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
     6061 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
     6121 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
     6181 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
     6241 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
     6301 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
     6361 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
     6421 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
     6481 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
     6541 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
     6601 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
     6661 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
     6721 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
     6781 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
     6841 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
     6901 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
     6961 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
     7021 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
     7081 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
     7141 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
     7201 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
     7261 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
     7321 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
     7381 tgaactcgca atggcgtccc gatcactgcg cggtcattgc cggggcgctg cgttacccac
     7441 gttatcgctg gtacgatcgt tttatgatca agctaattat gaagatgtca ggcggtgaaa
     7501 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
     7561 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
     7621 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     7681 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatc
     7741 tcaacagaga aaagtaa
//
LOCUS       DAZGCS010000173         7747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGCS010000173 DAZGCS010000000
VERSION     DAZGCS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.176"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..1086
                     /gene="torT"
                     /locus_tag="ACHK01_004600"
     CDS             58..1086
                     /gene="torT"
                     /locus_tag="ACHK01_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO8099411.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1059..1751)
                     /gene="torR"
                     /locus_tag="ACHK01_004601"
     CDS             complement(1059..1751)
                     /gene="torR"
                     /locus_tag="ACHK01_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO8099412.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            1871..3043
                     /gene="torC"
                     /locus_tag="ACHK01_004602"
     CDS             1871..3043
                     /gene="torC"
                     /locus_tag="ACHK01_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO8099413.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3043..5589
                     /gene="torA"
                     /locus_tag="ACHK01_004603"
     CDS             3043..5589
                     /gene="torA"
                     /locus_tag="ACHK01_004603"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO8099414.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGVIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5586..6185
                     /gene="torD"
                     /locus_tag="ACHK01_004604"
     CDS             5586..6185
                     /gene="torD"
                     /locus_tag="ACHK01_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO8099415.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(6278..6583)
                     /gene="cbpM"
                     /locus_tag="ACHK01_004605"
     CDS             complement(6278..6583)
                     /gene="cbpM"
                     /locus_tag="ACHK01_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO8099416.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(6583..7503)
                     /gene="cbpA"
                     /locus_tag="ACHK01_004606"
     CDS             complement(6583..7503)
                     /gene="cbpA"
                     /locus_tag="ACHK01_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO8099417.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1851 a         1966 c         2132 g         1798 t
ORIGIN      
        1 gttgagtcgc aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg
       61 cgcgtactgc tatttttact tctttccctt ttcatgttat cggcattttc ggctgataac
      121 ctgttgcgct ggcatgatgc gcagcattac acggtgcaag cctctacgcc gcttaaagcc
      181 aaacgcgcat ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg
      241 ttgaactatg gtatgcagga ggcagctcgc cgctatggtg tggatttaaa agtgctggag
      301 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
      361 ggcgcagagg ccattttgct cggtagtagc actacgtcat ttcccgacct gcaaaagcag
      421 gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccct ggtgaaaagc
      481 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
      541 gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataatgc cgggggcagt
      601 aaagagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
      661 attgcgcttg gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa
      721 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
      781 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
      841 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
      901 ggggaactgg cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
      961 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc
     1021 tcgctgtcac cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
     1081 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
     1141 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
     1201 cgtagcagac gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt
     1261 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
     1321 acattcaggc aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt
     1381 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
     1441 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
     1501 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
     1561 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
     1621 ttctgcataa tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc
     1681 tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
     1741 tgatgtggca tcagagggtt ttactcattc tgttcatatt ctgccgtaag ccgttcatcc
     1801 tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc ataattttac
     1861 aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt
     1921 actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt
     1981 tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt
     2041 gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg
     2101 ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag
     2161 caatgatatc taccagacct ttattgctca ctccattgat acacctgaaa aattcgaagc
     2221 caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac
     2281 ctgccgctcc tgccataact acgatgcgat ggatcatgcg aagcagcatc ctgaagcggc
     2341 acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat
     2401 tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc
     2461 cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc
     2521 gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa
     2581 acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca
     2641 gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt
     2701 gcagcagcag gtaaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag
     2761 caagttgcag gccactgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat
     2821 ctgggcttat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga
     2881 aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac
     2941 cagtctcgat aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga atgcgtctga
     3001 caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc
     3061 tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt cgccgggatg
     3121 ctggggccgt cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc gactgacgct
     3181 gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgacggtg
     3241 aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg
     3301 attgccggat tgccggatca cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc
     3361 gtggactggc tgcgtaagcg ccatctcagc gatacctccc agcgcggtga taaccgtttt
     3421 gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga acgcgtgcag
     3481 aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc
     3541 cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg
     3601 ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt tggctcaatg
     3661 gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg
     3721 ctgtggggct ctgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat
     3781 gtttatgaat attacgcgca gctaaaagcg aaagtcgccg ccggtgaaat tgaggtcatc
     3841 agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac
     3901 attgcggtta acccgcaaac tgatgtgccg ctgcaactgg cgctggcgta tacgctgtac
     3961 agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc
     4021 ctgccgtacc tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa
     4081 ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga
     4141 acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg
     4201 atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggctttggt
     4261 tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt
     4321 ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa aggctacagc
     4381 agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac
     4441 tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc cggaactaac
     4501 ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa gctggaaacg
     4561 gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat cgtgctgcct
     4621 gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc
     4681 gttatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt
     4741 ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa
     4801 atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt
     4861 catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg
     4921 cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg
     4981 ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta cgacgattgt
     5041 caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc cggctcgcag
     5101 aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt
     5161 gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt gttcattaac
     5221 ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct
     5281 cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct atgcacccgg cgtggcacga
     5341 attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa
     5401 tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc gcaggcgacc
     5461 agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gagcagtgga gcaggtgacg
     5521 gcgtttaacg gccctgtgga gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg
     5581 aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt
     5641 tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggctg
     5701 aatggttttc gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat gagctggaaa
     5761 actgtattgc cacgctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt
     5821 gcggcctgtt tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac
     5881 aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc agcggcaatt
     5941 tcaacgaacc ggcagaccat ctggcgatct atctcgagtt gttgagtcat ctgcattttt
     6001 cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga
     6061 cggcgctgtg gcaatggtta ccagagtttg ctgcgcgttg ccatcagtat gacagctttg
     6121 gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca
     6181 gataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc
     6241 agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg
     6301 aaagccgctg gcgtagcagg cggttttcct gcttcaggtg tgcaatatca tccattaacg
     6361 tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc
     6421 gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg
     6481 gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca
     6541 ggcaaaattc ggtaatagta aaagtcaccg taacattagc catcatgctt tcccccaatc
     6601 tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt
     6661 ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt
     6721 tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctgac tgcctggcgg
     6781 gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc
     6841 ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg
     6901 atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccaccgt tttcgcccgg
     6961 cgtcccctgg cctttcagac ggatgcgttg accattgccg acgcccgccg ggatcttcac
     7021 attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt
     7081 atagctgatg gtacgcttat gctcagtcag cgtttcttcg aggaataccg ccacttcgat
     7141 ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa
     7201 aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg
     7261 gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg
     7321 acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc
     7381 atccggttct ttgctgacat caggatggta tttgcgggca agtcgacgat aggcggtctt
     7441 gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc
     7501 catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta
     7561 taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat
     7621 cgcggagagg aggtcgccat caagatgggt tactgaacat attttaaaca ggtgaaaaag
     7681 ggtgagcgat ttttgatagt tgaaccaggc actttaagtt taactggggc gtcattattt
     7741 attaaat
//
LOCUS       DAZGCS010000174         7739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGCS010000174 DAZGCS010000000
VERSION     DAZGCS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.177"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(151..258)
                     /locus_tag="ACHK01_004607"
     CDS             complement(151..258)
                     /locus_tag="ACHK01_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8099418.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(663..1763)
                     /gene="chaA"
                     /locus_tag="ACHK01_004608"
     CDS             complement(663..1763)
                     /gene="chaA"
                     /locus_tag="ACHK01_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO8099419.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2033..2263
                     /gene="chaB"
                     /locus_tag="ACHK01_004609"
     CDS             2033..2263
                     /gene="chaB"
                     /locus_tag="ACHK01_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO8099420.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2421..3116
                     /gene="chaC"
                     /locus_tag="ACHK01_004610"
     CDS             2421..3116
                     /gene="chaC"
                     /locus_tag="ACHK01_004610"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO8099421.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3160..3513)
                     /gene="ychN"
                     /locus_tag="ACHK01_004611"
     CDS             complement(3160..3513)
                     /gene="ychN"
                     /locus_tag="ACHK01_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO8099422.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTHPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3698..5092
                     /gene="ychO"
                     /locus_tag="ACHK01_004612"
                     /gene_synonym="iatA"
     CDS             3698..5092
                     /gene="ychO"
                     /locus_tag="ACHK01_004612"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO8099423.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5093..5743)
                     /gene="narL"
                     /locus_tag="ACHK01_004613"
     CDS             complement(5093..5743)
                     /gene="narL"
                     /locus_tag="ACHK01_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO8099424.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5736..7532)
                     /gene="narX"
                     /locus_tag="ACHK01_004614"
     CDS             complement(5736..7532)
                     /gene="narX"
                     /locus_tag="ACHK01_004614"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO8099425.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7558..>7739
                     /locus_tag="ACHK01_004615"
     CDS             7558..>7739
                     /locus_tag="ACHK01_004615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099426.1"
                     /translation="MSNNLNECDDTFWNGSILGYWLKYTHTRKELLLICRVVSRFTSV
                     RVGILQHREKSHNFYE"
BASE COUNT         1962 a         1902 c         1972 g         1903 t
ORIGIN      
        1 tttcatgagt actctggatg atgcttccag ctcgggttgc caatatttac ttgtggaaga
       61 gataaagaca aaaatggccg caggctgtta cccctgcggc cggtttcggg cgcatattgc
      121 catcacggca gcctgacgcc cgttttcacc ttacttccgg ttacgccacc agctgacaat
      181 cgctgcggta ataatccccg ccaggatcgg tgctgccagg tcgtgccaga aagtcatggc
      241 aaactgcgcg agcgtcatat agccgccttg ttgtaatgac aacattttgc ggctattctt
      301 gaagtgtctg gtttcaagat tagcccccgt tctgttgtca ggttttacct ctcaacgtgc
      361 gggggttttc tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc
      421 ctcaccggat aattccggct tggtgtggat actacgtctc aattcatctt cacttcatcc
      481 ctgaaatgtt tgcaataaag agtacattcc ggcttttcaa cagctgttgc agtggtttcg
      541 tgcaggttgt ggtacaggct cgcaattctg attaacgagc tgtccaggta gtatgaagaa
      601 atacaataag caggaattta tcttatctcc cccgatttcc gtcatcggag gagatataac
      661 tgtcaggcaa atatcgtcat caaataggcg gcaaacagtg ccagatgcgc tgcgccattg
      721 agcacgttag tacgtccggt ggagaaggag atatggcaca gcactaaaga ggccaccatc
      781 accaccattt ctggcgcacc aagtgcaaac tgtaattcgt tacccgtcat aaaggcaatt
      841 agcgtgacga caggtacggt aagcgaaatg gttgctaaca ctgaaccaaa gaacagattc
      901 atcgcgcgct ggacctggtt gttcaacact gcttttaacg cacctaaacc ttccggcgac
      961 agaatcaaca gagccaccag gaagccagta aaggcgacag gggcattcat gctgtcgagc
     1021 aatgtctcca gcgggctggc attcattttg gtgactgcaa taacggcaat cagatggata
     1081 atcaaccaga tagcatgcca cacgctgcta tgggcagacg gtttaccgtg atgcgggtca
     1141 tcgtcatcac tgtcatcttc gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg
     1201 atcagcaaaa atacgccata cattgccgca gaaattaatg ctaccagtaa cgcctgaccg
     1261 gttgaaaaat tcgccgcagg cagagccatt ggaaatacca gtacgattat cgccaggggg
     1321 aacagcgcaa ttaaatactg cttgatacca aacagattca tatattgggt ggcaaactta
     1381 cgaccgccca acaataatga aaagccaacc agcccaccgg taacaatcat aatgattgaa
     1441 tagagcgtat cacgcattag cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc
     1501 aaactgactt caagaataac cactgaaagg ctaagaataa gcgaaccgta aggttctccc
     1561 aggcgatggg ctaatacgtc cgcatgacgg acaacactaa aggcgctact taaaatacca
     1621 ataagcgcaa gaagattgat ggcaatgacc actggtagtg tctggctgct tccccacagg
     1681 aacagcacta ccagcgccag aaccgggaaa ataagcgaag tctccttgtg gcgggttttt
     1741 accgcctctt gagcatttga cattatggtt atccctttgc agatgaattt atcgaaaatg
     1801 taaaaaatag gtaggaaaaa taacagaaag tgtctggata tcggtaacat tttacgaatt
     1861 tttaccccac tgtcgatttt ttactcattg ggacataaaa ataagtatta cgtttagaca
     1921 atgtttgttt tagcgtttct taagaacagt ctgacatgaa aattcttatg ttttggcaag
     1981 agtagatatt gttgaccaca cttaatgttc aactttgtaa aaggagtcaa cgatgccgta
     2041 taaaacgaaa agcgatctgc cggaaagcgt aaagcacgtt ctaccgtctc atgcccagga
     2101 tatctataaa gaagcgttca acagcgcatg ggatcaatat aaagataaag acgatcggcg
     2161 tgatgacgcc agtcgcgaag aaaccgcgca taaagtggcc tgggctgctg tgaagcatga
     2221 atatgccaaa ggggatgatg ataaatggca taaaaaatcg taaaaccggt agcttagtta
     2281 aagctattcg tgcggtgttg ccttgcaagt ggtccgtgga ttgcatattg tcccgttagt
     2341 ggtttcaaaa tgagcagtaa aaatgtccgg aagacaccaa aaagttgtcg cagggaagta
     2401 tgcagtggcg gaggtgtaag gtgataacgc gtgatttctt gatgaatgcc gattgtaaaa
     2461 cggcatttgg tgccattgaa gaatcactct tatggtcagc agagcaacgg gcggcttcgc
     2521 tggcggcgac gctggcttgt cgacctgatg agggaccggt gtggatcttc ggttatggat
     2581 cgttgatgtg gaatccggca ctggagttta ccgagtcgtg caccggtaca ctggttggat
     2641 ggcatcgcgc gttttgcctg cgcctgaccg ccgggcgcgg gactgcgcac cagccgggac
     2701 ggatgcttgc actgaaagag ggcggacgca ctacaggcgt cgcctatcga ctgccagaag
     2761 agacgctgga gcaggaacta accctgttgt ggaagcgcga gatgattacc ggctgttatc
     2821 tgccaacctg gtgtcagctt gatcttgatg atggatgcac agtaaacgcc attgtgttta
     2881 ttatggaccc gcgacatcca gaatatgaat ctgacactcg cgctcaggtc atcgcgccgt
     2941 tgattgcggc ggcgagcggt ccgctgggaa ccaacgcaca atacctgttt tcactggaac
     3001 aggagcttat caaactggga atgcaggacg atgggctgaa tgatttgctg gtatcggtaa
     3061 aaaaactgct ggcggagaat tatccggatg gtgtgttacg gccgggattc gcctgagtaa
     3121 acttccctca tagtggggcg tcagacgccc cctcaaacat taaaatgtga gcactttatc
     3181 ggctgacagc gtccattgcg ccagttccac aagagtaccg atttccaccc catcaataag
     3241 aggatgtgtg ctaatcccgc gcccgtcggt acaggttttg cacaatttca ccggtacatt
     3301 ctgagcggta aggatctcca gcatttgctg aatgttgtag ccttcccctg gtttttgccc
     3361 gcgcaacccg gctgtgaccg catcagacat caggaacaga cgcagatcca gattgctctc
     3421 ctgctctcgt aacgcaatgg ccagccgcaa gctgttaaac aaggattcgc tcccgtaagg
     3481 tgcgccattg gcaacgatca cgattttttg cattatttac tcctgtattc agggaattag
     3541 acattcatct tctatcttac tgcttctgca gcgtctgacc aatcggtcac atttttaagg
     3601 attttcctga aagcgcgaga aaatacgaca aaagttgcca gtaatcgtta ttctttaagg
     3661 ctatggtttt tcattattac cggaagttac cgacgttttg agccgtttcg ttcctcgcat
     3721 tatcccgttt tatttactct tgcttgtagc aggcggtaca gctaacgcac aatctacctt
     3781 cgagcaaaaa gcggcaaatc cctttgataa taacaatgat ggtctgccgg atttaggcat
     3841 ggctcccgaa aatcatgatg gggaaaaaca ctttgctgaa attgtgaaag atttcggcga
     3901 aaccagtatg aatgataacg ggctggatac tggcgagcag gcaaaagctt tcgcattggg
     3961 aaaagtccgc gacgcgctta gtcaacaggt taatcagcac gtagagtcct ggctatcacc
     4021 gtggggaaat gccagtgttg acgtcaaagt ggataacgaa ggacatttca ccggcagtcg
     4081 tggaagctgg tttgtgccgt tacaagataa tgatcgttat ctcacctgga gccagcttgg
     4141 tcttactcag caggatgatg ggttggtgag caatgtgggc gttgggcaac gctgggcgcg
     4201 cggcaactgg ctggtgggtt ataacacttt ttatgacaac ttgctggacg aaaatcttca
     4261 gcgagcgggc tttggtgccg aagcgtgggg cgaatatttg cgactatcgg caaactttta
     4321 tcagccgttt gctgcatggc atgaacagac agccacgcag gaacaacgga tggcgcgcgg
     4381 gtatgacctg acagcccgga tgcgcatgcc gttctatcaa cacctcaata ccagtgtcag
     4441 cgtagaacag tattttggtg atcgtgttga tttgtttaac tctggtacgg gttatcacaa
     4501 tcccgtcgcg ttgagtctgg gattaaatta cacccctgtg ccattagtca ctgtgacggc
     4561 ccagcataaa cagggtgaaa gtggcgagaa tcaaaataac ctcgggctga atcttaatta
     4621 ccgctttggt gtaccgctca aaaaacaact ttctgcgggc gaggttgccg aaagtcagtc
     4681 gttacgtggt agtcgctatg acaatccgca gcgaaataat ctaccgactc ttgagtaccg
     4741 acagcgaaaa acgttaacgg tgtttctggc gacaccgccg tgggatctaa aacctggcga
     4801 aacagtgccg ctgaaattac aaatccgcag tcgttacggt attcggcaac tgatttggca
     4861 gggcgatacg cagatattaa gtttgacgcc gggcgcacaa gccaacagcg cggagggctg
     4921 gacgctgatc atgcctgact ggcagaacgg ggaaggggca agcaatcact ggcgattatc
     4981 ggtggtggtg gaagataacc aggggcagcg tgtctcctcc aatgagatca cgctaacgct
     5041 tgtcgaaccg ttcgatgcat tgtcaaacga cgaactgcgc tgggaaccgt aatcagaaaa
     5101 tgcgctcctg atgcacccat accgctgctt ccacgcgaga cttgagcttc attttcttca
     5161 gcatgtgctt gacgtgcact tttactgtgc tttcggtgat atccaggcgg cgggcaatca
     5221 tcttgttcgg caaaccctgg gcaatcagct tgagaatatc gcgctcgcgt ggggttaact
     5281 ggttaacatc gcgctcagta gtggcacggt tagcgcgcaa gctggcggcc agaacaggcg
     5341 ttaatgcttc gcttaatacc atttcgccag cagctgcctg atgcaatgct ttcagcagat
     5401 cttccggttc catatctttt aacagatagc catccgcgcc gcgtttcagt gcggtgacca
     5461 catcttcttc atggttagag acgctgaata ccacaatgcg ccctgagagg gacttttcgc
     5521 gcagtttatc cagcgtttcc agaccgttca tgccgggcat attgagatct aacaggatca
     5581 gatcgggatc aagagactcc gccagttcaa taccctgttc gccattactc gcttcgccaa
     5641 ccacggtgat atctggtgcc atactgataa gctgttttac gccagttcgc agcatcgggt
     5701 gatcgtcaat cagcaggata gtagccggtt cctgattact catgggtatc tccttggacg
     5761 tctgtgaaag ttttttcggg aataaaggtg accaccactt cggtgccacc tgattcacga
     5821 cggcggacgc ggcaatcgcc tcgtaaactt tgcgcacggt cgcgcattat tatcatgccg
     5881 tagtgattgc tgcggatggc attttcaggc acgccgcagc cattatcctg aacggtcagt
     5941 tttacctgat tatcgttttg cgccaccgtc accacgacct cactcgcttg cgaatgtttg
     6001 agggcgttac ttaatgcctc acgggcaatt tgcaacaagt ggattgcctg atgcgaaggc
     6061 accagacgag gcggcaattg ataatccagc ttcaccggga agccaaattt ggcgctgtac
     6121 tcttcgcaac tcgcctccag cgccggacgt aatccaggct cggtgagctg caagcggaat
     6181 gtggtgagca attcacgcaa ctgcgcccag gatgcattca gttcgttacg gatctgactt
     6241 aacagttcgc ggctgctttc tggcagcgca tcgccctgca tctgtaaaca actcacctgc
     6301 atcttcatgc aagagagaga ttgggcaata gaatcatgca gttcgcgcgc aatggtggca
     6361 cgctcttcca tcacgatcaa ctgttgctga cgttcctgat ggcgatccag cgccagcgtg
     6421 gcggtgagtt gttcaaccag ggtatccacc agttgttgtt gatcatggct aagatgacgc
     6481 ccctgcggca gggtcgccag caaaataccg tactgcgtat gagagtcagc cagccgccac
     6541 ttcagggtcg tgccgcgatc gccaacgggt aatacgccgc gcgggcagag ctggcagcct
     6601 ttatcatcac aagtcatatc tggctggcag gtaaactcct gatgattctc ttcatcgtca
     6661 gtatcataaa cccgcagttc gatatcacgt agcagggtta aattctgtaa gccgttgagt
     6721 acaggtgaca ggcgttcaca cagcggggcg cgggaatgca aacggcggtt agcctgccat
     6781 aaaaaagaga ggatctgatt tttatgctcc agcccggcgg ttttctcctg aacccgctgc
     6841 tcaagtacgg cataactttc ggccagttct gcagacatat tgttcaatgc agttccaagc
     6901 atcgccattt cgttgcgccc gctgatgttc gcgcgttggg taaaatcgcg atggctgacg
     6961 gcactcgcca ttgccagcag ttgccgccac ggttgtagca gtcgcgcccg caaccaaata
     7021 atagtgaaca ccagtaaaag tgccataaat accgccatta cccgatggac cagtaccact
     7081 gtctcaatgc gcatttccgt ggtgcggtca aaaccagata ccagctgatc aagcccggca
     7141 acaaactggc tgacatccgc tgacaccgtt tcgcggtttt gtgcacgtat cagcgcaggg
     7201 atcagttcat tacgccagta atcttgtaaa ccctgtaatt gcgccagttg tccgtctcgt
     7261 tctgctgctc gagtcaactc ggcgctaaat gccgtttgtt ccatctcttt aattaagggc
     7321 ttgtctttct cgcttaatgg cactgctgcc aacagacggt aactttgcat gcgcagcgat
     7381 cccgctttgt tgatcgcatg ggcgctgccc tgaacgcctt gcaccagcca gccagaaacc
     7441 gccatccctg ccagtccaat agcagtagaa agcaacacaa taagcgcaac ctgattaacc
     7501 agggtgagcg gagagagaca acgtttgagc atgtaaacct cttccttcag gctttaaatg
     7561 agcaataacc ttaatgaatg tgacgataca ttctggaatg gcagtattct cggctattgg
     7621 ctgaagtata cccatacccg gaaggagtta ctccttattt gccgtgtggt tagtcgcttt
     7681 acatcggtaa gggtagggat tttacagcac cgtgaaaaat ctcataattt ttatgaaat
//
LOCUS       DAZGCS010000175         7680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGCS010000175 DAZGCS010000000
VERSION     DAZGCS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.178"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHK01_004616"
     CDS             complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHK01_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO8099427.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            549..2810
                     /gene="katE"
                     /locus_tag="ACHK01_004617"
     CDS             549..2810
                     /gene="katE"
                     /locus_tag="ACHK01_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO8099428.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDSTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHK01_004618"
     CDS             complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHK01_004618"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO8099429.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHK01_004619"
                     /gene_synonym="chbF"
     CDS             complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHK01_004619"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8099430.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ACHK01_004620"
     CDS             complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ACHK01_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO8099431.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ACHK01_004621"
     CDS             complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ACHK01_004621"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO8099432.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6336..>7680)
                     /gene="chbC"
                     /locus_tag="ACHK01_004622"
     CDS             complement(6336..>7680)
                     /gene="chbC"
                     /locus_tag="ACHK01_004622"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO8099433.1"
                     /translation="ASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLIN
                     NVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALA
                     EERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTF
                     IVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTIS
                     TPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVE
                     AALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIF
                     QINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLG
                     AFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2053 a         2016 c         1719 g         1892 t
ORIGIN      
        1 tcaaaggcag ctttttgtta caggtggttt gaatgaatgt agcaacgaaa tgcagaattt
       61 caggtcatgt aactcccggc aaaaccggga ggtatgtaat ccttactcag tcacttcccc
      121 ttcctggcgg atctgatttg cccaacgttg ggcagattca ggcacagtaa acgccggtga
      181 gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac
      241 atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac
      301 atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa
      361 acgcatatta aaaattagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct
      421 ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcgggatc tggctggtgg
      481 tctatagtta gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga
      541 cgagttcaat gtcgcaacat aacgaaaaga acccacatca gcaccagtca ccactacacg
      601 attccagcga agcgaaaccg gggatggact cactggcacc tgaggacgga tctcatcgtc
      661 cagcggctga accaacaccg ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc
      721 ctgatacacg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt
      781 atgcgctgac cactaatcag ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg
      841 gtaaccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg
      901 accatgagcg catcccggaa cgtattgttc atgcacgcgg atcagcagct cacggttatt
      961 tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca
     1021 aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggcgctggc tctgccgata
     1081 ccgtgcgtga tatccgtggt tttgccacca agttctatac tgaagagggt atttttgacc
     1141 tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg
     1201 ttcatgcggt aaaaccagaa ccgcactggg caattccaca agggcaaagt gcccacgata
     1261 ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt
     1321 cggatcgcgg catcccccgc agttaccgca ccatggaagg cttcggtatt cacaccttcc
     1381 gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag
     1441 gtaaagcctc actcgtttgg gatgaagcac aaaaactcac cggacgtgac ccggacttcc
     1501 accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct
     1561 tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacca
     1621 aactgatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca
     1681 atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc
     1741 ctggtctgga cttcaccaac gatccgctgt tgcagggacg tttgttctcc tataccgata
     1801 cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac cgtccgacct
     1861 gcccttacca caacttccag cgtgatggca tgcatcgtat ggggatcgac actaacccgg
     1921 cgaattacga accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga
     1981 aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgtgagcgca
     2041 gtccatcgtt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat
     2101 tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt
     2161 atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg
     2221 tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg
     2281 acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg
     2341 tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg
     2401 ccattctcaa ggccctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg
     2461 gtgaagtgac tgcggatgac agtacggtgt tgcctatagc cgctaccttt gccggtgcac
     2521 cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca
     2581 acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg
     2641 cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga
     2701 ttgtggaagc tgacagcgcc gacggtagtt ttatggatga actgttaacg ctgatggcag
     2761 cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa
     2821 ggtcacgtcc cctggggagt gaccttacca ccttacttac acattaagat aactacccaa
     2881 ccgataacca cgctcagcaa ttgcatattt caacgacgct gatgtcagca cttccagctc
     2941 cgttaaccgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
     3001 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
     3061 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
     3121 attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc
     3181 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
     3241 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
     3301 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
     3361 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
     3421 agtaaggaca aagtgcatcc ctatggccag actcggttca tcacgactca actgcaccgc
     3481 atggtcaata gcctgcccat tcaccagcgc cgtcgttgat gtgacaatcc cattgcgaca
     3541 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
     3601 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
     3661 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
     3721 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
     3781 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
     3841 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
     3901 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
     3961 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
     4021 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
     4081 cggattttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gtgctcgtgc
     4141 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttctgct tgaagtaata
     4201 cagcagataa gaacatggca gcagatttaa cgaacgaatt aagccctcac taaatggcag
     4261 atcgaaaata ttttttacag aggacgcttt taactgccct gacgccacac catcaagcaa
     4321 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
     4381 gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa
     4441 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
     4501 ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
     4561 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
     4621 aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg
     4681 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
     4741 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
     4801 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
     4861 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
     4921 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
     4981 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
     5041 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
     5101 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
     5161 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
     5221 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
     5281 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
     5341 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
     5401 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
     5461 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
     5521 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
     5581 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
     5641 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
     5701 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
     5761 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
     5821 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
     5881 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
     5941 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
     6001 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
     6061 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
     6121 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
     6181 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
     6241 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
     6301 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc attagcgata
     6361 tcttcttcgc tctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
     6421 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg cgaccagcaa tgcggcgacg
     6481 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
     6541 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
     6601 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
     6661 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
     6721 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
     6781 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
     6841 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
     6901 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
     6961 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
     7021 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
     7081 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
     7141 cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata
     7201 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca
     7261 cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata
     7321 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
     7381 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
     7441 ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc gaggcgaata
     7501 cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca
     7561 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
     7621 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
//
LOCUS       DAZGCS010000176         7592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGCS010000176 DAZGCS010000000
VERSION     DAZGCS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.179"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(187..309)
                     /locus_tag="ACHK01_004623"
                     /pseudo
     CDS             complement(187..309)
                     /locus_tag="ACHK01_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(634..818)
                     /locus_tag="ACHK01_004624"
                     /pseudo
     CDS             complement(634..818)
                     /locus_tag="ACHK01_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1024..1230
                     /locus_tag="ACHK01_004625"
     CDS             1024..1230
                     /locus_tag="ACHK01_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099434.1"
                     /translation="MNTPVSLMDDQLVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1319..1921
                     /locus_tag="ACHK01_004626"
     CDS             1319..1921
                     /locus_tag="ACHK01_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099435.1"
                     /translation="MKQQYQTRYESLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWISFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLKRK
                     SEKELEEWIIDRQKQNAALTDLMDQYVLLAYRTTVDDNRN"
     gene            2211..2865
                     /locus_tag="ACHK01_004627"
                     /pseudo
     CDS             2211..2865
                     /locus_tag="ACHK01_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3538..5046
                     /gene="ltrA"
                     /locus_tag="ACHK01_004628"
     CDS             3538..5046
                     /gene="ltrA"
                     /locus_tag="ACHK01_004628"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO8099436.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRLIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTKH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTGTRNTYTSRFTEVAMAFASI"
     gene            5130..5609
                     /locus_tag="ACHK01_004629"
                     /pseudo
     CDS             5130..5609
                     /locus_tag="ACHK01_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6650..7522
                     /locus_tag="ACHK01_004630"
     CDS             6650..7522
                     /locus_tag="ACHK01_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099437.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYLSYQKRILN
                     TLISQGVDTLKINNFLAETDQRYFDSLPSENDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNVDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSMNEGISNDRVI
                     ESALDMIYRSKIKK"
BASE COUNT         2087 a         1827 c         1897 g         1781 t
ORIGIN      
        1 tattcgttgt cacaataccg cttaacgttc gcggtattat ccaggttacc aacaaccgga
       61 gaagacctaa aaagaatcac acagagcaga taacaccaga ccgttgtcat taccggatac
      121 tcaggtgacc agactggctc cacacgcgta aagtttgtcg tcagttctgc cagccgatcg
      181 accgcagaag gcttccagct ccgcaggggt aagtttataa cgtgtgcggg taatcacttt
      241 ttccagtgtc tcccgggatg aacaacgacg gaactgatac agccagtctt ctttggtttt
      301 aacttccatt agcctctcat cacttagcct ctcatcactt tatgctgcaa ttaacgtgat
      361 actgccagca taccgcacca gactggcatt ctggtaccca ccccaaactc attactcccg
      421 taccaccact taaccggtct ctcagactgt cactcaccag gtcggatacg tttaccgctc
      481 agacggatgc ttaatgcact ctgttcacgt gtgttgcagg tacgttttat atacgcaggt
      541 tatacgccag ctatcaggga tagccctgtg agtacaacgt aatctgttgc tactgcgttc
      601 tcatgcccac tgatcacact cacagtgttt atttcacaga tatgaactca tgtgtttgat
      661 ctctcttata tatttataat accaatcaga cagtcttatc ccatcggtga tacctgtcgc
      721 ctgcccgcta ggaacagact gacttactgt aaccgcctcc caaaacaact ctgcaattat
      781 cagaacgccg gtttttcccc catcctcacg aaaatcatcc cttctttatc gcgcgtgcgt
      841 gcagaagcat cgtttcgcgg tagccaccgg gtatctcagc atcatgctcc ctgtttatct
      901 cccggcagga acattcccct actcattttt tacgtttctc cccggttgtt atgccggtgc
      961 aggtgatgcg ttgttttcat cacaaacgtc ggttgagatt aacaacatcc gaaggagatt
     1021 ttcatgaata ccccggtttc gctgatggat gaccagctgg tcgacatggc atttatcact
     1081 caactgaccg gcttaaccga taagtggttt tataagctca tcagagatgg tgcctttccg
     1141 gcccctatca aaatgggtcg cagctcccgc tggctgaaaa gcgaagtgga agcctggctg
     1201 caggcacgca ttgcacagtc ccgtccgtag ttcataaccg ttatccgttt acctgcagca
     1261 gacgcaattt ccggcctgtc ggcggcattc tgctgcctgt tatatccccg agaggaagat
     1321 gaaacaacaa tatcagaccc gctacgaatc gctccacgaa agctaccaga aatggctgac
     1381 cggcttcacc cggcacgctg tatcctgggg agtgtgtcat ccgaatatct actacttcca
     1441 taatctgacg cccggatgga tatcattcaa tggtgaaaaa cccgagatcg ccatcgtacc
     1501 ccagagtctg caccggctga tttatggccc tgacaaacgg accacgccgc cgctggatga
     1561 tgatctggtg gtgaacttat gcaccagtga acatctgctg gttcatcatc cgatgctgga
     1621 aggcattctg ctgtctgaat gtgaacgtct gaaacatcat tctctggcaa ataaactgat
     1681 cagcctcttc cgtcagtttg gtggcacaga gttgcgcctc aaactggtct ggctttgctg
     1741 gcttgattta atgaccggaa acagccttga agactggaca gagaacctga aacggaaatc
     1801 agaaaaagag ctggaggaat ggatcatcga ccgtcagaag cagaacgcag cactgacgga
     1861 tctgatggat cagtacgtgc tcctggcata ccgcacaacg gttgacgata accgtaactg
     1921 atgccggttg cttccggaag gtgcggcaga atacgggaat tatgctcccg gccccgcctg
     1981 ctcacctgca gagcgtgttc gtcgtcatca tactgagcca tgatgaatgg cttaagggca
     2041 tcctgtcatt actgaactgg catgaacacc accagtggat gaaagccacg gacagggatg
     2101 ttctttacag ccagctacac gccacgttct ctacggactg aatatgcagg cagaggtgaa
     2161 ccgccccgga aatcctggag actaaactcc ctgagaaaga ggtaaacagg atgactaaaa
     2221 atacacgttt ttcccccgaa gtccgtcaac gggcagttcg tatggttctg gaaagtcagg
     2281 gcgaatatga ctcacaatgg gcggcaattt gttccattac cccaaagatt ggctgtacgc
     2341 cggagactct gcgtgtctgg gtacgccagc atgagcggga taccggaggc ggtgatggtg
     2401 ggctcaccac cactgaacgt cagcctctga aagagctgga gcgtaaaaat cgtgaactgc
     2461 gccgcagtaa cgatatcctt cgccagactt ccgcttatat gcagtgaact gcatattgcc
     2521 ccgtcaacgt attaccactg tcagcaacag cgacatcgtc ctgataaacg cagtacccgt
     2581 gcgcagcgcg atgactggct gaagaaagag atactgcgcg tatacgatga gaatcatcag
     2641 gtatacagtg tacgtaaagt ctggcgtcag ttgttacggg aaggtatcag agtggccagg
     2701 tgcactgtgg cacgtctcat ggcggttatg gggcttgccg gtgttctccg gggtaaaaag
     2761 gtccgcacta ccgtcagccg gaaagccatt accgcaggcg accgcgtaaa ccgtcagttc
     2821 ggggcagaac gtcctgacca gttgtgggtg gctgatttta cttacggtgt gcccggagtt
     2881 cagggcgggc atggatgctt aaatgaaccg cgagtctgtc tggaatattg aaccggtaac
     2941 tcacgatgag aaacccaaca atcccaccgg gtgtgacggt ggagaacctg agcggcagtg
     3001 acctgcggca tgcccgcagg gtgatgtaac ccgctgacaa cggggattga ggcgagatca
     3061 ctaagccgag atgatcctca aggttaagta ctgaaaggtt gaagaacatg aacccgttaa
     3121 tccgcctctg tgggttgaaa acgtcaccac ggcctacgtg atctgacagg ccgtgcagga
     3181 ggaactggca gtgatacgta agcactgccg gtcgaaggtg ttttgacatg tatgcgaaac
     3241 accggggcag cagcgtctat cacgcttgcg ttgctgactt ctgccaactt gcggcaagca
     3301 aggataaaga gtgcgacggg cagcctcctc agtatgcctg agtccaggca ggtaaaccgg
     3361 ggaaggtcag cgacggatgt taagggggca tggctccgat gacgcgctgg ctggcggagc
     3421 ttccgtagta gtccgcgatg gggaaagccc attacatggc gaagggaagc agtttgaatg
     3481 tgtttgcgac gtgaattaac tgacctaacg aggtgaagac ctttgataat cagcgaaatg
     3541 caacgcaagc tggccacatg ggcagccacc gatccgtccc tacggattca acggctgctg
     3601 cgtctgataa cacaaccaga atggctggct gaagcggcgc ggatcacgct ttcatcaaag
     3661 ggggcccata cccccggcgt tgatggcgtg aacaaaacaa tgctacaggc cagactggct
     3721 gttgagctgc aaatcctcag ggatgaatta ctctcaggcc actaccagcc cttgcccgcc
     3781 agacgggttt acatccctaa aagcaacggc aaactgcgac cactgggtat ccccgcgttg
     3841 cgggatcgta ttgttcagcg ggccatgctg atggcgatgg agccgatatg ggagagtgat
     3901 tttcatacgc tctcatatgg cttccggcct gagcgcagtg tccaccacgc gatccgcacg
     3961 gtgaaattac agctcacaga ctgtggtgaa acccggggac gctgggtgat tgaaggcgac
     4021 ctgtccagtt acttcgacac cgtacatcat cgactgctga tgaaggctgt acgccgcagg
     4081 atcagtgacg cacgtttcat gactctgctg tggaaaacca tcaaggcggg acatatcgat
     4141 gtcggtctct ttcgggcggc cagtgaaggt gtgccacagg gcggtgttat atcgccgcta
     4201 ttatcgaaca tcatgctgaa tgagttcgat caatacctgc atgagcgcta cctgagcggg
     4261 aaagccagaa aagatcggtg gtactggaat aacagtatcc aacggggccg aagtacggcg
     4321 gtcagagaaa actggcagtg gaaacccgcg gtggcgtact gccgctatgc cgatgatttt
     4381 gtcctcatcg tcaaaggcac caaagcacag gcggaagcca tcagggagga gtgtcggggt
     4441 gtgctcgaag gcagtctgaa actcaggctg aacatggata agactaaaat cacccatgtt
     4501 aatgacggct ttatctttct ggggcacagg ctcattcgca aacgcagtcg ttatggcgag
     4561 atgcgagtgg tctcaacgat cccgcaggag aaagccagaa acttcgccgc atcgctgaca
     4621 gcactgttat caggcaacta cagtgaaagc aaagtcgata tggctgaaca actcaaccga
     4681 aaactgaaag gctgggccat gttctatcag ttcgttgatt ttaaggccaa agtcttcagt
     4741 tatatcgacc gtgtcgtgtt ctggaagctg gctcactggc tggcccgcaa ataccgtaca
     4801 ggtatcgctt ccctgatgag gtggtggtgt aaatcaccga aaccgggtca gagcaaaacg
     4861 tgggttttat ttggtaaaac caaacacggc aagctcagcg gcgaaatact gtaccggttg
     4921 gtggggcaag gcaagaagct gttccgctgg cggctacccg aaggtaatcc ctatctgagg
     4981 acggggacca gaaacacgta tacatcgcgc tttacagaag tggcaatggc gttcgccagc
     5041 atttaaatgg agagccggat gcgctgaaag gtgcacgtcc ggttcgggga ggagaggcag
     5101 ggaaatagtc cgactacgcc ctgcctctta ctctactcag cacatggcag ggcttcgttt
     5161 atgtggcgtt catcattgat gtgttcgccg gatacatcgt gggatggcgg gtctcatcgt
     5221 ccatggaaac gacattcgtg ctggatgcac tggagcaggc agtatgggac cgtcgtccgt
     5281 ctggcactat ccatcactgt gataaaggtt ctctgtatgt atcgctggcc tacacgcagc
     5341 ggcttaagga agcaggatta ctggcatcaa cagggagcac aggcgactcg tatgacaacg
     5401 cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt aatagctgga
     5461 aaaaccggac agaagtggaa ctggccacac taacgtgggt ggactggtat aacaatcgac
     5521 gattgctggg aaggctggat catatccctc cggcagaagc agaaaaagct tattatgctt
     5581 ccatcagaaa cgatgatctg gcagcctgag ttcacaaata aaacaatctc caggaaaccc
     5641 ggggcggttc aaatttcgca gagggctggc ttctgatgct gacgctaacg tgcttttccc
     5701 acctgctaac cagaaagtaa tctgcccagc ataacagtaa ccccatcagc ttaaaatcaa
     5761 taacgccctg taaaacctga agtaacagtc cgggaactcg tatcatgcta acatatcagt
     5821 gttgtgaagc agtcgacaac gagcttcaga cggtacacac gtcagttcat gcagaaatag
     5881 cggccatatc aggtctggct gtcagcgaac cacgcagcct gagtacattg ttgtaaatta
     5941 aacgactggt gatttttaaa ttttttcttt gccaccaccg tgaactaata ccgaaaaaac
     6001 agtagtctca ggaagcgagt tctgaaagtc agctttatta aaataagcta ttagaccggg
     6061 taagtaatgt caacgaatga aatgttatga taattctgga taccatatct gtaagagtga
     6121 ttaatgagga aagtgatgac ttttaattat tttatctccg gtaacgtaaa accagactta
     6181 tcgtaaatga aattatgaag tcctctgtta acgtcatgga gagacgcatc aaaatacagg
     6241 atacaatgat tctcacaatg aaactcatcg tctctggtga aattcatatc aacatttacc
     6301 ttaataacct gacgcgggat taactgtgtt gtttaaggca aaggggggag ttgcccgaag
     6361 gcgaaaacga aagtaagtta ataataacag gatgagagga gaaagcgctc ttcccggaaa
     6421 ataaaaagag cggttcactc ctgtgaatgc acttcaaccg tcctggcaaa gaatgtactg
     6481 ttataaaaaa ctcccccagt cagcagagac aaaaagataa catgaggtac ctcccacccc
     6541 cttaatttat acacgtgaaa tgaactgaaa tttatgagaa aatcatgcta aagatgcttt
     6601 gattttactt aaaatcacgt gtgattatcc tgactgaagg agagatacta tggttaaggt
     6661 tgtggaagat gaaagaagca gaatacgata tcttgaaaga cgcttaaatg aaaatgggtt
     6721 ttatttacca tcaagcctgg ccgataaaga ttatttatcg tatcagaaac ggattttaaa
     6781 cactcttatt tcacagggtg ttgacaccct aaaaataaat aattttctgg ctgaaacaga
     6841 ccagcgttac ttcgattcct taccatctga gaatgatctt aactggtatc gtaatgatgc
     6901 ccgcgccagt ttatggctga cttgtgaatt atatgaaatg attaagataa acggttatga
     6961 aaataccctt acctgtttat ctccggagtc actaccttct caccacagcg tgagggtaga
     7021 tgccatacgc aggtgtattg ataactggcc tttcatttta tacacaccat caaattacct
     7081 aaaccagaaa agtattgaat ggaccacact gctcgaaaaa gatgatattt tcagagaggt
     7141 taaggccaga aatgttgata tctgctcatg gctcaaaaaa tatattcagg aaaagacgaa
     7201 tatttccctg aactatgtct gtggagaatc atctgaagag attatggcct ggtgttatgc
     7261 cagttacttc acatggaaaa aaaacaacca gaattcacca gactcagtag aactgtttac
     7321 ccggaagttt aaaagcgcct gggctaccca aaaaaacaga ataaaaaaca gggttgataa
     7381 aaagctgcgc cccctgaacg taaatatttc acaggaggca tatgacaaac tgagaaaatt
     7441 atccatgaac gagggtatct caaatgatcg ggttattgaa agtgctctgg acatgattta
     7501 ccgtagtaaa attaaaaaat aaaaccattc aaaataattt cagacagata ttatctccgt
     7561 gaatcccccg ccacctttcc ggtgtgcggg gt
//
LOCUS       DAZGCS010000177         7543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGCS010000177 DAZGCS010000000
VERSION     DAZGCS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.180"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..469
                     /gene="yecR"
                     /locus_tag="ACHK01_004631"
     CDS             146..469
                     /gene="yecR"
                     /locus_tag="ACHK01_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO8099438.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            640..1137
                     /gene="ftnA"
                     /locus_tag="ACHK01_004632"
     CDS             640..1137
                     /gene="ftnA"
                     /locus_tag="ACHK01_004632"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO8099439.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1175..1414)
                     /gene="yecH"
                     /locus_tag="ACHK01_004633"
     CDS             complement(1175..1414)
                     /gene="yecH"
                     /locus_tag="ACHK01_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO8099440.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1605..2816
                     /gene="tyrP"
                     /locus_tag="ACHK01_004634"
     CDS             1605..2816
                     /gene="tyrP"
                     /locus_tag="ACHK01_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO8099441.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2878..3543)
                     /gene="yecA"
                     /locus_tag="ACHK01_004635"
     CDS             complement(2878..3543)
                     /gene="yecA"
                     /locus_tag="ACHK01_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO8099442.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3739..3825)
                     /locus_tag="ACHK01_004636"
     tRNA            complement(3739..3825)
                     /locus_tag="ACHK01_004636"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3789..3791),aa:Leu,seq:taa)
     gene            complement(3838..3911)
                     /locus_tag="ACHK01_004637"
     tRNA            complement(3838..3911)
                     /locus_tag="ACHK01_004637"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3877..3879),aa:Cys,seq:gca)
     gene            complement(3966..4041)
                     /locus_tag="ACHK01_004638"
     tRNA            complement(3966..4041)
                     /locus_tag="ACHK01_004638"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4006..4008),aa:Gly,seq:gcc)
     gene            complement(4193..4741)
                     /gene="pgsA"
                     /locus_tag="ACHK01_004639"
     CDS             complement(4193..4741)
                     /gene="pgsA"
                     /locus_tag="ACHK01_004639"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO8099443.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSASRADLLDQ"
     gene            complement(4798..6630)
                     /gene="uvrC"
                     /locus_tag="ACHK01_004640"
     CDS             complement(4798..6630)
                     /gene="uvrC"
                     /locus_tag="ACHK01_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO8099444.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6627..7283)
                     /gene="uvrY"
                     /locus_tag="ACHK01_004641"
     CDS             complement(6627..7283)
                     /gene="uvrY"
                     /locus_tag="ACHK01_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO8099445.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7425..7496
                     /locus_tag="ACHK01_004642"
                     /pseudo
     CDS             7425..7496
                     /locus_tag="ACHK01_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1822 a         1935 c         1782 g         2004 t
ORIGIN      
        1 ataaaaatga taatcaaaaa acagcccccc tatttctgac acctacagat ggcaagaaat
       61 agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc
      121 tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt cattaataac
      181 gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg
      241 tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg atcgttatgt
      301 cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc tatacccacg cggtcccctt
      361 tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga atacggaatt
      421 cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa tattttaatc
      481 agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc ccattcgtat
      541 ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca aattatagtg
      601 acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt
      661 gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg
      721 agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc
      781 caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta
      841 ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc
      901 caggaaacct ataaacacga acaattaatc acgcagaaaa ttaacgaact ggctcatgct
      961 gcaatgacca atcaggacta cccaacattt aatttcctgc aatggtatgt ttctgagcag
     1021 catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc
     1081 ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc
     1141 tctcaggcgg caggttttct gccgccgttt caaatcagtg acggcaaatc ttactctgat
     1201 cggtcgaaaa accttcttct gaaggtataa atttgccttt tgctgccaga aacgctacca
     1261 gctctcccgc agtcatccct tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac
     1321 gcgctttaat tgctgcttcc agactggcat gcgtatattg ctcgcctgat tcaatcatca
     1381 tatttaacac ttcatgaccg tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg
     1441 tagcgattgc cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt tttattgtac
     1501 atttatattt acaccatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg
     1561 cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc
     1621 tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc
     1681 tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt
     1741 tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg
     1801 gtcttggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca
     1861 gtatgatgtt cttaatgtat gctctgactg cggcatacat cagcggtgcc ggtgaattgt
     1921 tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt
     1981 tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta
     2041 accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta ttgctgctac
     2101 cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg gctctgtcag
     2161 caatcccggt gatttttacc tcatttggtt ttcacggtag cgtgccgagt attgtcagct
     2221 atatggatgg caacgttcgt aagctacgct gggtgtttat aaccggtagt gcgatccccc
     2281 tggtggcata tattttctgg caggtggcaa cgcttggcag cattgattca acaaccttta
     2341 tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg
     2401 tggcctctcc gcatgttgag ctggcagtgc atttatttgc tgatttagcc ctcgccacgt
     2461 catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttattt cagcgttcaa
     2521 ataccgttgg tggacggttg caaactggtg caattacctt tctgccgccg ttggcgtttg
     2581 cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg
     2641 tactggcatt gattatccct tcgctgttga cctggcaaag cagaaagcac aatcctcagg
     2701 cgggttaccg ggtgaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg
     2761 ctgtgattgg cgtgcaattt ttgattgcgg cagggttgtt accagaagtg gggtgatcag
     2821 atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatt tgacctctta
     2881 atgcaggcag cactgcttaa atttcttacc actaccgcac gggcaaggat cgttacgccc
     2941 cggtttctct tctgctttga taggttgctg aacagctttt tcctgcggat gcgccatcca
     3001 gtacgcatgt agatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc
     3061 tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc
     3121 cagcgctggt tttaacgaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat
     3181 atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc
     3241 gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg
     3301 ttgaaaagcc agattcataa agcgcgtcat ctctttctct gacgcccagc gcggcacata
     3361 gtcagcccca ccccacacgg caaccagcca ctgttccggt tcaatctctt gcggagaact
     3421 caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt
     3481 gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt
     3541 catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat
     3601 cagagatact ttttgagtgg ctttgctggt gattaaaaat tagggagggt gtaacgacaa
     3661 gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat tgctttgatt
     3721 ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag cgcgaacgcc
     3781 gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg aagaaagtgg
     3841 aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca taaccgcttt
     3901 gttaacgcgc caaattcttc atgcctttca gccagacatc cgcttgacgc cgatgtcttt
     3961 taaactggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
     4021 taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc
     4081 gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc
     4141 aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac gatcactgat
     4201 caagcaaatc tgcacgcgaa gcgctcaaat attgcaacat tgaccacaga gtcagtaccg
     4261 cagccacaaa gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca
     4321 gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca
     4381 cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac
     4441 gggcgatcat cgttgccgcc ggtagggtca cccaccagct gtgataatgc tcggttacca
     4501 gcaccatggc gatagccacg agaactttat ctgctacagg gtcaaggaaa gcaccaaacc
     4561 gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga
     4621 cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata
     4681 caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca
     4741 taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca
     4801 atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc
     4861 aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat
     4921 ttgccgacgt tttggcccga cgccttcaat ggtttccagg gagctggtat ttttgacctt
     4981 cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg
     5041 gataacatgc agcgcgggag aatctggcgg caaactgaat ccctcacctt ccggctcaaa
     5101 gaacagtgtt tccagcccag ccttacgatc tactcctttg gcaacgccaa gtagcagtgg
     5161 atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg
     5221 gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc
     5281 ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgcctgt
     5341 aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga
     5401 agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc gcttcacttc
     5461 cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag attgctgcga
     5521 aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct
     5581 atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc
     5641 ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg
     5701 catctggctg ccttgtaaat agaactggcc tacgaaggtt tctaccacct cgctcagttc
     5761 cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat
     5821 gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa cgtcgaggtc
     5881 gtcgccggta ttggaaacga attgtttttc ggtgacgcgt cgcaccgcct gaatttggtc
     5941 gcgaatacgt gcagcttctt caaactccag attctggctg gcagtttcca tacgactaat
     6001 gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg
     6061 ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat
     6121 ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat
     6181 ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg
     6241 gccgaaatat tcacctttgg catgcttcgc accacgatgc atcgccagac gcgggtgggt
     6301 atcaccactc aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg
     6361 cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtgtgagt
     6421 aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag
     6481 gttgctacgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat
     6541 aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa
     6601 aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga
     6661 ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac
     6721 atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca
     6781 ttgaccttct ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg
     6841 gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgatg
     6901 tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac aacttcctgc
     6961 ggagccgcgc ctttgctgag gtagcccgca gcaccggcct gcatgacttt cgctggtaaa
     7021 gggttttctg tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt
     7081 ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca
     7141 acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc accgacgact
     7201 tttataccct ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca
     7261 tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata
     7321 gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt
     7381 attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca
     7441 ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac aaaaattgac
     7501 tttattcagc aaaaatgaaa atcagcccgc tgaatatgta aca
//
LOCUS       DAZGCS010000178         7516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGCS010000178 DAZGCS010000000
VERSION     DAZGCS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.181"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..838
                     /gene="gatY"
                     /locus_tag="ACHK01_004643"
     CDS             2..838
                     /gene="gatY"
                     /locus_tag="ACHK01_004643"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO8099446.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GC"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHK01_004644"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHK01_004644"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO8099447.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..2603
                     /gene="gatA"
                     /locus_tag="ACHK01_004645"
     CDS             2151..2603
                     /gene="gatA"
                     /locus_tag="ACHK01_004645"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO8099448.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2634..2918
                     /gene="gatB"
                     /locus_tag="ACHK01_004646"
     CDS             2634..2918
                     /gene="gatB"
                     /locus_tag="ACHK01_004646"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO8099449.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARGDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2922..4277
                     /locus_tag="ACHK01_004647"
     CDS             2922..4277
                     /locus_tag="ACHK01_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO8099450.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4325..5365
                     /gene="gatD"
                     /locus_tag="ACHK01_004648"
     CDS             4325..5365
                     /gene="gatD"
                     /locus_tag="ACHK01_004648"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8099451.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5471..6244
                     /locus_tag="ACHK01_004649"
     CDS             5471..6244
                     /locus_tag="ACHK01_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8099452.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHK01_004650"
     CDS             complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHK01_004650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO8099453.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1889 a         1695 c         1954 g         1978 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg gttatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgctaggg
      841 cagggcataa cgcacctgcc atttaataag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggaatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccatgttga agataccgcc aatactttac gtacgcatca aaaggccttt attgcccgtg
     1501 ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggc gtggaatttg
     1561 atcacagcaa tattatccat tatcagccgc aggaagcgca gccgctggcg caatggatag
     1621 aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acggcttatt
     1681 gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta acctttgctt
     1741 tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc cctgaaaatc
     1801 gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
     1861 aatattatcg tacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagtcgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atcttgaagg cgtggacatc ccactgggca tgattagtca gtatcttccc aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gctgattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgccaaaggt gtggttcatg atacctggcc acaggcatta attgccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggttattgcg ttgattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
     2581 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
     2641 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
     2701 ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt cgggttaatg
     2761 aaatagaaac ctatatggat ggtgtgcatt tgatatgtac cactgccagg ggggatcgca
     2821 gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc ggtatcgaag
     2881 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
     2941 gttatattct cgacctcggc cctacagtga tgctgccggt tgtcatcatt attttttcta
     3001 aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
     3061 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg gcggcgaaag
     3121 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
     3181 caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg atattggtta
     3241 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     3301 ggcatatgac cttcaccggc gcgttgctgc acctggcaac cggttcatgg atgataggga
     3361 tggcaggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     3421 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
     3481 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     3541 ttaaatttag cgccgacgat attcagcgca aatttggtcc atttggcgag cctgtcaccg
     3601 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     3661 tgcagctggc ggtaaaaacg gcggcagtga tgctgctaat gccacgggtg attaaaccca
     3721 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     3781 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
     3841 tatcggcaag cctgattttt atcccgctca ccattttaat tgctgtttgt gttccgggta
     3901 atcaggtgct gccgtttggc gatcttgcca ccatcggctt ctttgtggcg atggcagtcg
     3961 ccgtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatc atgagcatca
     4021 ctctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     4081 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     4141 tactgattca ggttttctcc ccgcaaaata ttcccggatt cattattatc ggcgcaattt
     4201 atcttaccgg tattttcatg acctggcgta gagcgcgtgg ctttattaaa caagagaaag
     4261 tcgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
     4321 ttttatgaaa tcagtggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     4381 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     4441 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     4501 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac atcctggcga
     4561 tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt tgaaagggtt
     4621 ttattcccag tgcgcaaaat atgattttat tggctcacgg cgtgatggtg gatttgccga
     4681 atatattgtc gttaagcgga aaaatgtctt tgctctacct actgatatgc ctattgagga
     4741 tggggctttt attgaaccga ttaccgttgg cctgcatgct tttcatttag cgcaaggttg
     4801 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg ccattcagtg
     4861 cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagttcag aaaaactggc
     4921 actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
     4981 aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga cggctggcgt
     5041 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
     5101 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     5161 agagctgacg gttatcggca gttggatgaa ctactccagc ccttggccgg ggcaggagtg
     5221 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     5281 ccgtggaagc tttgaaagct tcgcccaggc ggtgcgtgac atcgctcgta atgctatgcc
     5341 gggcaaagtg ttgctcattc cctgaaaccg cgggccagcg tgatgctggc ccggtattgt
     5401 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     5461 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     5521 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     5581 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     5641 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     5701 tcagctcgcc agcgcgccga aaaacagaat agcgcaggcg gcagtcaaaa tgatccatga
     5761 agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg aaggattaat
     5821 gaccgccaaa aatatcacgg tgattaccaa cagtctcccg gctgcgtttg ccctttccga
     5881 aaataaagac attactctgg tcgtctgcgg tggcacggtc cgccataaaa cgcgatctat
     5941 gcatggatct attgctgaac gttccctgca ggatattaat gccgatttaa tgtttgtcgg
     6001 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
     6061 cggtgcgatg gtcaccgccg ctaataaagt gattgccgtt ctcgattcat cgaaatttaa
     6121 ccgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     6181 tgctgtatca gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     6241 gtagtattgt tctgtatgcc ggataaggta tttacaccgc atccggcatt tcggattctc
     6301 cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt ggtaatcgac
     6361 aacgcaacgc cgccggaagt atttcaatat gaaagttttg tccgctcaac ggttcgccat
     6421 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
     6481 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     6541 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc ggacacaatt
     6601 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     6661 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     6721 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     6781 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     6841 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     6901 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
     6961 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     7021 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     7081 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     7141 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     7201 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     7261 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     7321 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
     7381 ctaacggctg ccaccgataa atttcaaaaa agagcatatg cctaatattc aaccaaacag
     7441 tggcatctta aatatactat tccaaatata ttaaagcccc catggagtta ccctgaaggg
     7501 cctcaatgtc cgtaat
//
LOCUS       DAZGCS010000179         7398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGCS010000179 DAZGCS010000000
VERSION     DAZGCS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.182"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..377
                     /locus_tag="ACHK01_004651"
     CDS             <1..377
                     /locus_tag="ACHK01_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFO8099454.1"
                     /translation="SAIERGLSGRKDKIQYYPLSLNQYNDGSHTMLTISGFFSSEQEY
                     IELSKACDLSNWQFYSTNWEDVQEIAIPTLTIKEKINLDSKLPDKEAYQAAAEEFSLN
                     ENERDNYYKYYRLYPNFQRIMV"
     gene            complement(345..1496)
                     /locus_tag="ACHK01_004652"
     CDS             complement(345..1496)
                     /locus_tag="ACHK01_004652"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO8099455.1"
                     /translation="MNVIDLFSGVGGLSLGAARAGFDVKMAVEIDQHAINTHAINFPR
                     SLHVQEDVSLLNAEIIKGFFKNDMPIDGIIGGPPCQGFSSIGKGNPDDSRNQLYMHFY
                     RLVSELQPLFFLAENVPGIMQEKYSGIRNKAFNLVSGDYDILDPIKVKASDYGAPTIR
                     TRYFFIGVKKSLKLDISDEVFMPKMIDPVTVKDALYGLPDIIDANWQSDSESWRTIKK
                     DRKGGFYEKLWGQIPRNVGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGETDK
                     ISRSTRLDPNGFCPTLRAGTARDKGSFQAVRPIHPYHPRVITPREAARLQGFPDWFRF
                     HVTKWHSFRQIGNSVSPIVAEYILKGLYNLLNKRVQPEYLNHNSLEVRV"
     gene            complement(2090..2194)
                     /locus_tag="ACHK01_004653"
                     /pseudo
     CDS             complement(2090..2194)
                     /locus_tag="ACHK01_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2252..2413)
                     /locus_tag="ACHK01_004654"
     CDS             complement(2252..2413)
                     /locus_tag="ACHK01_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099456.1"
                     /translation="MTTITKERIELFIKNPVENGLTRGEQMELARIALASLEAEPVGD
                     FYEYKPDDW"
     gene            complement(2424..2687)
                     /locus_tag="ACHK01_004655"
     CDS             complement(2424..2687)
                     /locus_tag="ACHK01_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099457.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            complement(2689..2906)
                     /locus_tag="ACHK01_004656"
                     /pseudo
     CDS             complement(2689..2906)
                     /locus_tag="ACHK01_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            complement(2939..3151)
                     /locus_tag="ACHK01_004657"
     CDS             complement(2939..3151)
                     /locus_tag="ACHK01_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099458.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            complement(3257..3679)
                     /locus_tag="ACHK01_004658"
     CDS             complement(3257..3679)
                     /locus_tag="ACHK01_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO8099459.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
     gene            complement(3695..4456)
                     /locus_tag="ACHK01_004659"
     CDS             complement(3695..4456)
                     /locus_tag="ACHK01_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8099460.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPAELPEETTMATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PTGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIFDSSSRIVSEK"
     gene            complement(4479..5225)
                     /locus_tag="ACHK01_004660"
     CDS             complement(4479..5225)
                     /locus_tag="ACHK01_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8099461.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELVTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEK"
     gene            complement(5232..6020)
                     /locus_tag="ACHK01_004661"
     CDS             complement(5232..6020)
                     /locus_tag="ACHK01_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099462.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQINTVSFPDSAIPPGFRG"
     gene            complement(6098..6520)
                     /gene="ydaT"
                     /locus_tag="ACHK01_004662"
     CDS             complement(6098..6520)
                     /gene="ydaT"
                     /locus_tag="ACHK01_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099463.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(6517..6771)
                     /locus_tag="ACHK01_004663"
     CDS             complement(6517..6771)
                     /locus_tag="ACHK01_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099464.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            6851..7270
                     /locus_tag="ACHK01_004664"
     CDS             6851..7270
                     /locus_tag="ACHK01_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099465.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT         1928 a         1837 c         1457 g         2176 t
ORIGIN      
        1 gatcagcaat agaacgtggt ctttcaggac gtaaggataa gattcagtat tacccattat
       61 ccttaaatca atataatgat ggcagtcata ccatgttgac aatatcaggt tttttctcct
      121 cagaacaaga atatatagag ttatctaagg catgcgacct ttctaactgg caattttatt
      181 caacaaattg ggaagacgtg caagaaatag caataccaac actaacaatt aaagaaaaga
      241 taaatttaga tagtaaactg cctgataaag aagcatatca ggcagccgct gaagagttct
      301 ctttaaacga aaatgaacgg gataactatt ataaatacta taggttatac cctaacttcc
      361 aaagaattat ggtttaaata ttcgggctgt actcttttat ttaataaatt gtacagcccc
      421 tttaatatat attcagcaac tattggtgac acgctatttc ctatttgtct gaaactatgc
      481 catttagtta catgaaagcg aaaccaatca gggaagcctt gtaatctagc agcttctctt
      541 ggtgtaatca ctcttggatg ataagggtgg atcggcctga cggcttgaaa gcttccttta
      601 tctctagcgg ttcccgccct taaagtcggg caaaaaccat ttggatctaa tcttgtagat
      661 cttgaaattt tatcagtttc cccaaaagat aaggaagcat aacgctcttg gacgattttg
      721 ctatgtaatg ttccggtaca tccagatatg atattatttt ttaacttagc gatcgattct
      781 gtatcaccaa cattccgagg gatctgcccc cataattttt cataaaatcc cccttttcga
      841 tcttttttaa ttgttcgcca actctcagag tctgattgcc aattggcatc gataatatct
      901 ggtaatccat acaaagcatc ttttacagta accggatcaa tcattttagg cataaatact
      961 tcatctgaaa tatcaagctt caatgatttt tttacaccga taaaaaaata tctagttcga
     1021 atagttggag caccataatc agatgctttt accttgatgg gatcaagaat atcataatca
     1081 ccgctaacca aattaaatgc tttatttcta atgccagaat atttctcttg cataatacct
     1141 ggaacatttt ctgccaaaaa gaataatggc tgtaattctg atactaaacg gtagaaatgc
     1201 atgtaaagct gattcctgct atcatcagga ttccccttgc ctattgaact aaatccttgg
     1261 cacggaggac cgccaataat accatctatg ggcatatcgt ttttaaaaaa acccttaatt
     1321 atttctgcat ttagtaaaga aacatcttct tggacgtgca aacttcttgg aaaattaatt
     1381 gcgtgagtat taatagcatg ttgatcaatt tcaactgcca ttttaacatc aaatccagca
     1441 cgtgcagcac caagacttag acctccaacg ccagaaaaca aatctattac attcatatag
     1501 ttgcaacgtc tcttattagt ttctatgtat tataacacaa ctgttcttga tgttttaacc
     1561 ccataagata acctcataaa ggctatccct tgaaaatacc ggatcgcata aatttaatct
     1621 ttcaaaacta cttgtctatt aatcgagcaa tttaaggttg ttttagtcat gtcgataccg
     1681 acatagttaa ctaaacccat ttcatcgtcc aacaaatggg ggtaatacac tttgatttat
     1741 agtcgggtgg gtttttctct atctgccttt ggtgttcatg cccaaggcag atagcctcaa
     1801 gcacccgcag caattctact taactatcct tttcccgcaa atcgttttta tccccagcgg
     1861 caaatcgaat acacaaccag cgccaccgcc atcgcaattc ctaccgttgt gaatgcttca
     1921 ggccaggtca tcgtgaaaca tcctccacgc ttataagtcc gcttcgctcc aggtagtcca
     1981 tcactttatc cggcaatttg cagcccggtt tcgctttctt cagttgacta accaattgtt
     2041 taaccagcat tgtcaattcg cgaacttgtt tcccgggctc ccctttgccc tgaagcaggg
     2101 cagcgcggca agcgttccat ccctcagcat atgtttcagt tacaccatcg agatggcatg
     2161 taagcaaatc catttcttcc ggcactacgg gcgctggaaa aacggcatag ggtggcgtcc
     2221 attttggcgc tttatctcca gccgaacgct gctaccagtc atccggtttg tattcataaa
     2281 aatcaccaac tggctctgct tccagcgatg ccagcgcaat ccgtgccagt tccatttgtt
     2341 caccacgggt aagcccgttt tcaaccggat ttttaatgaa caattcaata cgttctttgg
     2401 taatagtggt catgtgttac tccttaaccc gcagtgcttt caactgatga ggggaacaaa
     2461 atcttttcat caaaccctgc attcatatca tgaacagcaa cacaccaatc catcgacgaa
     2521 cgattatcaa gagcctccat gatttcatcc atgcggcgta ggtcatacag gtaaatgctt
     2581 ttatcgccaa tggtgtaaaa gccaattttt ttcggtgatg gacagcgatc aagaacttcc
     2641 tgtaattcgt tcaaccatgc ccgttctttt tttgtcaaag ttgccatatc agttttcctt
     2701 atacggatta attttattgt gcagtgtgtt gaatgacgcc cataccacgt cgttatacaa
     2761 ttcaataact ggctcaatta tttttccgat tatccagact aggattaacg gggatatcgg
     2821 tatcatcaac acgataaaca gaatgagaaa cagaaattct gttgttctac tctttcgcgg
     2881 atattttttc taaataatgt gaccattcat taccgccctt tcgggcggcc tcctgacatt
     2941 aatcgttgtg ataactcata gcttcatttg cagcatcaac tggatcaacc tccctccagc
     3001 aataatttgg gtcggctcct tcaggcgtcc acggttctaa ttcatttttt gccgcattct
     3061 catcgccagt aattttaaaa atctgctcag agaattttct tgcccactcg ttatattttt
     3121 ccgcattaat ggctttctgt gtatttaaca taaatatacc tccagttaag gattaaattt
     3181 tatttacagt gctgaactta attattcaga tttggattat gctttctctt cacgaagttc
     3241 cgattgttaa tttggctcac aacagcacct tctgaaaatt accctgatag aaagccagta
     3301 cacgctgcat agcttcgctc ttccggcact cgctacagat tatgttcaga cgcctgtcgt
     3361 agcggcgtat ttctccgtct ggtaatgacc agataagatc cggatcaacc acaaccggtt
     3421 tcttcacctt tgcccttgat agttttttgc gggcgttttg ccagtcttta cgcgcctgct
     3481 cagacgggaa taatccgtag cctgaattgt aaacatcacc actggcgacc agttctctgg
     3541 cgagagtgct tatgtaatac cttgatgcac cggttttagc ctccagagcc cgtaacgtct
     3601 cgcgaccgct cagacgtaca agttcaacaa cctgcccttt aattttttcc cgctcttctg
     3661 gtgtaaatac ttttgccata ggtgcctccg gcaatcactt ttccgacaca atacgactgg
     3721 aggaatcgaa aatctgtcga acaatatccc ggtgcttgtt cagctcccgc agcgcggcgc
     3781 agactcgctc ccacttctgg acatgatttt tcgcccgacg cagttcgcgg tttgccatat
     3841 gcagcgatgg taaaaccagg tcatccgctc gcgtttcagt aaacgatggc agcgactgca
     3901 caatgtccgc caccgtttct gttttaatat cttcctgtgt tgcagcctcc tgtactggta
     3961 acgcaacacc tgtgggctga ggaaaggcct taccatcagt ttccgctacc gatgctgctt
     4021 tcggctctgc tggtaaatta tcgcccggta tgcagtaacg aaatttaccg ttctggttta
     4081 cgcgaatcag gcgtcctttg ctgatagcca ttgccagcgt tgaagctact ttgcgtgatg
     4141 tggtaccgaa caatgtagcc aactcatccg ccgtttgtgg gccacgttgt tcaatcgtcg
     4201 cggttaaatc gcactctgag attttcgcta ctgttgccat ggtggtttct tccggcagct
     4261 ctgccggcgc tggctgttcc tgctgaacgt tgttatcagc cacgcgccag gtgtatacgc
     4321 ttttatcaac gaagccagcc tttttcagtt cccacagctc gttcagcact tcttctcgac
     4381 tgatatcaag tcgcgtagcc agttctaccg acgtggcttt tcccatcgct ttcagtgcgt
     4441 caaaaacggt ctccattaaa atttcctccc ggtaaaaatt acttctcaat acctggctga
     4501 ccaacattcg ggcgccagct ctcccagtta aaattcaccc agcgcccgcc gttcatggtc
     4561 atgcgatcca taatccgctc gccgagcaat gttttcatgg cctcatagtt caggtttgtc
     4621 agcatcccca cgctgcgcat tgacgctgtc cggcgatcaa caatctggtg cagtaccacc
     4681 tgctcgtttt tcgtctcgcg ctgaatacca atttcatcaa gaaccagcag atcaacttcg
     4741 cacagttccc gcaaaaattt ttcgcctgac tgcccgtcgt catagctggc gtgcagggcg
     4801 ctcataacat cagccacggt aaccacaatc actgtctggc catctttcag caggcgattc
     4861 ccgatagccg ccgccagatg gttttttccg gtaccaggtt ttccgctgaa cgcgaaattt
     4921 gtgcatccgg taaccagttc gtcagcgata gatttcgcct gactcaacgc gtatcgctgg
     4981 ccgtcgttct gcacctggta attcgcaaac gagcatttgc ggtgcaacgg ctgtatgccg
     5041 gagcgattca ggattttttc cacccgcaac tgacgattct gacggttgat ctcctcacaa
     5101 cgtttctggc cttctgcaag ttgccactcg cgccactccg ccaccgtcct gaatggcgcg
     5161 gttacatgtg gcggggccag tctgcggata cgttccagaa cgccgcctgt cgcaatattt
     5221 ttcatggtct gttaccccct gaagcctggc gggatcgcac tgtccggaaa cgagacggtg
     5281 ttaatctgtc ggagcaacgt ctctggccga acacctttcg gcgcgaacaa gccctggtat
     5341 tcattggcga tgctgtgtcg aatcacctgc tcaggtgaaa aaccctgctg gcggaatttt
     5401 tccagctccc gtatcgcccc gttagcgccc tgctccgttc gaatcggttt tcgcaatgcc
     5461 tgcctgaact gaacccactc atgccagagt gtttccggca accagtcagg cagctcgata
     5521 gcctccggtt cgaatttttt agacgctcgt ttttggcgag ggggatttag ggggagataa
     5581 gtatttatat cttcctcttt ctcttcctct ggtaacgctt tttgatccgt ttttgtaacg
     5641 ctggcagcgt taccttttcg tttcagttct cgtatttttg ttactctctc gtttgtaacc
     5701 gcccgttttt tagagctttt cccgttatgg cgctcaaagt taggaagcga caacacacca
     5761 ttagtttcga ccagccatcc aacctgaatt aacgcatcag caaaaccagc cataaaagtg
     5821 atgcgatcta ttgcactttt tgtaacgccg cgagcgttac actctgcgtt accgtctatc
     5881 atttgttgat ccgcccatgc ccagaagcga atgactttcc ctaatgcggc atctggatca
     5941 atattcagaa tctcagcaag cctgaatatt tccggcttat ccggcgtaat aacttcgagc
     6001 tttatccagt ttgaagccat ttgttttcac cttgtaacgc tcgcagcgtt acatttaact
     6061 gataccgaac aaaacagttc ggtacgatta atttcaatca atgcactacg acagaatcgc
     6121 taggagaacc gccgccgctg aaatgtgctt tacggtaaac ggcctggact gcatcatcat
     6181 gcgcatcaat tgccgtactc agtgcatcct gtgccgccag taatgcacgg cgttccaggg
     6241 tatcgaagat gcagagtcgg tgacgcagct cgcgaggaag aattgccaga accgcaggga
     6301 tcagtttctg aattttttcc ctttgcgcat tcgtttcacc tttcaaccaa cgatgataga
     6361 tattctgctg attgttccag tccttgcctg gtaccagggg caattcgccg cccccctggc
     6421 gcagatattc ttcagtaatt gcgttagcga cccacgcctg ccctttttcg gctgccaggg
     6481 ctaacaacac tgattcgatg tgctcatgct tgattttcat gaatcaactc ccatcagctt
     6541 tttcgtagta gttttatttc tgccaatagt taaaattgca tcggcagaaa ataatccgtt
     6601 tgatgcatga gcgatttttt cagcgtaatt tgtttcgccg gtatattctg tgcgaggcaa
     6661 tttaccgtta tccatccatt tgtagattgc tctttggctg acaccacaaa cgtcggccac
     6721 aacagaaacg cgaacagttt tgattacatc ttcaagtgtt ttctggttca tatcaccctc
     6781 acaatgtgaa ctttgagtac atgctataac agaactgaca gtacattcaa gagcgaatat
     6841 cattgaactt atggttcatg aagataaagc gcgtaaagag ttcgccagta ggcttgcgct
     6901 agcctgtgaa aacgctggtt atgaacaaca tggaaggcag gcagaaattg cccgtcgaat
     6961 gaaattaaca ccaaaagcgg ttagcaaatg gtttaatggt gaaacaattc ctcgccgaga
     7021 gaaattaagg gaattagcaa cactcattgg aacaacacca acctatcttt tgggagagga
     7081 tacagaagaa agtggacaga tacgtttcta tcaggagtta aatccaagac aaaaaatcat
     7141 cattgacctt ctggacgagc tccctgacag tgagacagat gaacttttaa aaactcttga
     7201 ggagaaaaaa cagaagtaca atgcaattta cgaagagtta gcacgaaaga aaaaacaaaa
     7261 agcctcttaa accagcataa atccggtagc gtccccctcc gggtttgtgc ttcactttat
     7321 cccgtctcat ttttttatac ataaaatgta cttaaagtac tttacaatga tgaacacaaa
     7381 gtacattata tacctacc
//
LOCUS       DAZGCS010000180         7126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGCS010000180 DAZGCS010000000
VERSION     DAZGCS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.183"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(316..1452)
                     /locus_tag="ACHK01_004665"
     CDS             complement(316..1452)
                     /locus_tag="ACHK01_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8099466.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAVALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1455..1817)
                     /locus_tag="ACHK01_004666"
     CDS             complement(1455..1817)
                     /locus_tag="ACHK01_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8099467.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2354..4267
                     /gene="mtlA"
                     /locus_tag="ACHK01_004667"
     CDS             2354..4267
                     /gene="mtlA"
                     /locus_tag="ACHK01_004667"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO8099468.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4362..5510
                     /gene="mtlD"
                     /locus_tag="ACHK01_004668"
     CDS             4362..5510
                     /gene="mtlD"
                     /locus_tag="ACHK01_004668"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8099469.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5510..6097
                     /gene="mtlR"
                     /locus_tag="ACHK01_004669"
     CDS             5510..6097
                     /gene="mtlR"
                     /locus_tag="ACHK01_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO8099470.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6109..6318)
                     /gene="yibT"
                     /locus_tag="ACHK01_004670"
     CDS             complement(6109..6318)
                     /gene="yibT"
                     /locus_tag="ACHK01_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO8099471.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6603..6965
                     /locus_tag="ACHK01_004671"
     CDS             6603..6965
                     /locus_tag="ACHK01_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO8099472.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1815 a         1788 c         1805 g         1718 t
ORIGIN      
        1 attaaatatt ttttaaatgt gactgtcatc cttcagataa taaagccatc atcgcaatag
       61 atggaaaagc actcctgctc ttttttgatt agagccgtcg caggaggaac aactcatatc
      121 atcattgcga tctcgacgat gtatagtctc atcctgcggc agaacaagac ggataaaaaa
      181 tccaatgaga gcacactcat ccagaactta ttaacataat gaatattaaa gaaaaatcat
      241 cgcagctaat gaagtaatat gcaaaaataa ttagtcctta caaggagcaa agttccataa
      301 tctcccccct ccccctcaat gatccaaata aagataatgc atccagctgg tcattcttag
      361 cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc
      421 gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag
      481 cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat accccctgcg cctgataaga
      541 accgcctggc acgacaggta aaatactagt cagtttgccg tgaaacacct gcccaggtag
      601 cgcgttaaac accacttccg catcatcgcc gggtttcaga cgtaacagcg agttttgccg
      661 aaattgggcg acaatttgcc gtttttgctc ggggatgaag accatcaccg gacgcagcgg
      721 caaggcaact gcgtatgtac ctgggcggat cagtacctga gtaacgtagc cattgctcgg
      781 cgcgcgaatg acagtctgct caaggttata ttttgcttca gtaagttgcg ctcttaagct
      841 cacaatctgc gattgctcgc cgttaaccat actgtcgagc tggctctgga tctgcgcctg
      901 ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc
      961 gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaagt aacgttgata
     1021 atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt
     1081 gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct
     1141 gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat
     1201 aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg
     1261 gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa
     1321 aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg tccactgatt
     1381 taccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag ttaaaacaat
     1441 caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt ctcggcggcg
     1501 agttgatgct ccagccggga aattcggtca gaaagtgcgg cgatttccgg gtcagtcgct
     1561 ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg atagagcgtc
     1621 gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac ccagcccgcc
     1681 gtatgaatag cgtcggcatg gggatggttg cgctttttgg caatcagata agggatgtca
     1741 tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac tcccaacgcg
     1801 aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt tttgggctgg
     1861 cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga ttctcgccat
     1921 aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg attgtatgac
     1981 gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata tcttgtgatt
     2041 cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc acatttaaat
     2101 gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt tgtgatgaac
     2161 gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca caaaaggccg
     2221 tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag gtccggctac
     2281 ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag caaacataag
     2341 aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg gtcgtttcct
     2401 cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt
     2461 atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg ggccgatgat
     2521 cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag gcggcgaacg
     2581 tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag acatgccgat
     2641 gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc acttcgaccg
     2701 ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact tctccgcagg
     2761 catcatcggg atgatcctcg ctattctggc atttctcggc attggcccga ttgttgaagc
     2821 cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca tgctgccgct
     2881 ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg
     2941 tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct
     3001 gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt tctttggtcg
     3061 tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg ggggtatcca
     3121 cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg
     3181 cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt ctccggcatc
     3241 tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt tcgctaacat
     3301 cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt tgctgaaaac
     3361 cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc aggacatgaa
     3421 agctgagtct aaaggcacat ctccgctgtc tgcgggcgat gtgactaacg acctgagcca
     3481 cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tccagtgcga tgggcgcagg
     3541 cgtgctgcgt aagaaaattc aggatgcagg tctgtcacag atttcggtca ctaacagcgc
     3601 gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc tgaccgaacg
     3661 cgctatgcgc caggttccgc aggcgcagca tatttcgctg accaacttcc tcgacagcgg
     3721 cctgtacacc agcctaaccg aacgtctggt tgctgcccag cgccatactg aaaacgaagt
     3781 gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc tgttcaaact
     3841 gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag cgattcgttt
     3901 tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc aggcgatgct
     3961 ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc cacacggtac
     4021 ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt acccggaagg
     4081 cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta ttgcagcccg
     4141 taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg atgagtccgt
     4201 catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgt tggcaggtcg
     4261 taagtaatcc aatcctaccc tctcctcacg gagagggttt gggtgaggga aaagcctcac
     4321 cccagccctc tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg
     4381 gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac
     4441 tgacgtttgc cgatgtcaat caggtggtac ttgatgccct gaatgcccgt catagctatc
     4501 aggttcatgt ggttggtgaa accgagcagg tagataccgt ttccggcgtc gatgctgtca
     4561 gcagcattgg tgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg
     4621 ttggcccggt tgtgctggaa cgtattgctc cggctatcgc caaagggctg gtgaaacgta
     4681 aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg gtacgcggta
     4741 ccacgcagct gaaaggccat gtgatgaacg ccctgccgga agacgccaaa gcgtgggtag
     4801 aagaacacgt tggctttgtc gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg
     4861 caactaacga tccgctggaa gtgacggtag aaactttcag cgaatggatt gtcgataaaa
     4921 cgcagttcaa aggcgcactg ccgaacatcc caggcatgga gttaaccgac aacctgatgg
     4981 catttgtcga acgtaaactc ttcaccctga acacgggtca tgctataacc gcgtacctcg
     5041 gaaaactggc tggtcatcag accattcgtg acgctattct cgacgagaaa atccgcgcgg
     5101 tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat caagcgctac ggctttgacg
     5161 cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac ccgtatctga
     5221 aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc
     5281 tgatcaagcc actgctcggt acgctggaat acagccttcc gcacaagaat ctgattcagg
     5341 ggattgctgg tgcaatgcac ttccgcagtg aagatgatcc gcaggctcag gaactggcag
     5401 cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt cttgatgcca
     5461 acagcgaggt tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca
     5521 ggcgcaggac accctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca
     5581 aacccaggcc tttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag
     5641 ctttctgatc accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt
     5701 attccgcaaa gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgacgg
     5761 tccgctgggc gatctttctg tgcgtttaaa actcatttac gggttgggcg tcattaaccg
     5821 ccaggaatac gaagatgcgg agctgctgat ggcattgcgt gaagaactaa atcacgacgg
     5881 caacgagtac gcctttaccg acgacgagat ccttggaccc tttggtgaac tgcactgcgt
     5941 ggcggcgtta ccaccgccgc cacagtttga accagcagac tccagtttgt atgcaatgca
     6001 aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc ctttcactga ctgagctgat
     6061 ttccagaatc agcttaaaaa aagcctttca aaagtaagca acgtctgctt actgcccctc
     6121 tacctgcttc ggccgataaa gccgacgata atactccaga cgttgtagat ataacggcac
     6181 gctttcatcg ggtattccgg acggaatcgc gttacgggga ggaagttttt tcagatactc
     6241 ccggaacgcc tggcttgatg ccatgaaatc tacggcttta tcgataagaa gcggaacgtt
     6301 ttcacctaat ttgcccatga tgatatctcc gtattacccc gcgccgggaa tgcgcggccg
     6361 ccaattttag tttaggttcc cggtaaagat gacattaagg aaaacgtgct gaatcctcaa
     6421 taatcatccg ctgctgttta ttatcttttt tctatagatt tcttaattaa tcaataaatt
     6481 gcgtagtttt taagcatgtt aatgattacg ttacagaact attgcaatgt catcagttgt
     6541 gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc
     6601 acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag cgatcgtctg gacgccatcc
     6661 gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa
     6721 aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg tgaagttcat agccaaaaac
     6781 tgagtaaaga agcacagaag ctgatgaaga tgccgtttca gcgcgcaatt accaaaaaag
     6841 agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa
     6901 tgaccgcgct gggccgtgaa atgggcctgc aggagatgac tggattctct aagactgcgt
     6961 tttaagcgcc tcagacgact ggttcgatca tatcaacccg catctggctt gccttagctg
     7021 gtcaatgcgg ggactctcct gtctactcgc ctaattacca aacaattcac tccctttact
     7081 tcgatttacc gctggttgat attctgaaaa tgcattttta ccgaaa
//
LOCUS       DAZGCS010000181         7121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGCS010000181 DAZGCS010000000
VERSION     DAZGCS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.184"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            558..1547
                     /gene="araF"
                     /locus_tag="ACHK01_004672"
     CDS             558..1547
                     /gene="araF"
                     /locus_tag="ACHK01_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO8099473.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMEVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MEITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1617..3131
                     /gene="araG"
                     /locus_tag="ACHK01_004673"
     CDS             1617..3131
                     /gene="araG"
                     /locus_tag="ACHK01_004673"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO8099474.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3146..4132
                     /gene="araH"
                     /locus_tag="ACHK01_004674"
     CDS             3146..4132
                     /gene="araH"
                     /locus_tag="ACHK01_004674"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO8099475.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4299..5099
                     /gene="otsB"
                     /locus_tag="ACHK01_004675"
     CDS             4299..5099
                     /gene="otsB"
                     /locus_tag="ACHK01_004675"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO8099476.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5074..6498
                     /gene="otsA"
                     /locus_tag="ACHK01_004676"
     CDS             5074..6498
                     /gene="otsA"
                     /locus_tag="ACHK01_004676"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO8099477.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6505..6933)
                     /gene="uspC"
                     /locus_tag="ACHK01_004677"
     CDS             complement(6505..6933)
                     /gene="uspC"
                     /locus_tag="ACHK01_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO8099478.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1809 a         1648 c         1837 g         1827 t
ORIGIN      
        1 gcgctttttg tatcttgttg atattaatca atgaatttga attcatgatg aagtgatgat
       61 tttataaaac gttttttcat ttttgcgaga tggctctcat tatacgtgtt ctgttaatta
      121 atgagcacag tgataattaa tggcgaaatg aatgacgtgc attttccaca tctttgagtt
      181 gcggttatta caccatttca aaaactcaac gccaggtaat gcggcctatt gactggttaa
      241 aaagaagaca tcccgcatgg gtaccaaaga caacaaggat ttccaggcta atcttatgga
      301 ttaatctgct gtgcattcga caatttgtct gacaaattgg ctttccctta tgtcttttcc
      361 cgctaaattt atgcacgttc tcactgtaat tctgcgatgt gatattgctc tcctatggag
      421 aattaatttc tcgctaaaac tatgtcaaca cagtcactta tcttttagtt aaaaggtaat
      481 gctttgtttt ccgattaatt taacgaatgt cattcgtttt tgccctacac aaaacgacac
      541 taaagctgga gagaaccatg cacaaattta ctaaagccct ggcagccatt ggtctggcag
      601 ccgttatgtc acaatccgct atggcggaga acctgaagct cggttttctg gtgaagcaac
      661 cggaagagcc gtggttccag accgaatgga agtttgccga taaagccggg aaggatttag
      721 ggtttgaggt tattaagatt gccgtgccgg atggcgaaaa aacattgaac gcgatcgaca
      781 gcctggctgc cagtggcgca aaaggtttcg ttatttgtac tccggacccc aaactcggct
      841 ctgccatcgt cgcgaaagcg cgtggctacg atatggaagt cattgccgtg gatgaccagt
      901 ttgttaacgc caaaggtaag ccaatggata ccgttccgct ggtgatgatg gcggcgacta
      961 aaattggcga acgtcagggc caggaactgt ataaagagat gcagaaacgt ggctgggatg
     1021 tcaaagaaag cgcggtgatg gagattaccg ccaacgaact ggataccgcc cgccgccgta
     1081 ctacgggatc tatggatgcg ctgaaagcgg ccggattccc ggaaaaacaa atttatcagg
     1141 tacctaccaa atctaacgac atcccggggg catttgacgc tgccaactca atgctggttc
     1201 aacatccgga agttaaacat tggctgatcg tcggtatgaa cgacagcacc gtgctgggcg
     1261 gcgtacgcgc gacggaaggt cagggcttta aagcggccga tatcatcggc attggcatta
     1321 acggtgtgga tgcggtgagc gaactgtcta aagcacaggc aaccggcttc tacggttccc
     1381 tgctgccaag cccggacgta catggctata aatccagcga aatgctttac aactgggtag
     1441 caaaagacgt tgaaccgcca aaatttaccg aagttaccga cgtggtactg atcacgcgtg
     1501 acaactttaa agaagaactg gagaaaaaag gtttaggcgg taagtaattt gccggaaaaa
     1561 ttcccctctg catgatgcag agggggtgtg aacgaccagt gattcacgga gacgttatgc
     1621 aacagtctac cccgtatctc tcatttcgcg gcatcggtaa aacgtttccc ggcgttaagg
     1681 cgctgacgga tattagtttt gactgctatg ccggtcaggt tcatgcgttg atgggtgaaa
     1741 atggcgcagg aaaatcaact ctcttaaaaa tcctcagcgg caactatgcg ccaaccacgg
     1801 gttctgtagt gattaatggg caggaaatgt ccttttccga cacgaccgca gcacttaacg
     1861 cgggtgtggc gattatttac caggaactac atctcgtgcc ggaaatgacc gtcgcggaaa
     1921 acatctatct cggccagctg ccgcataaag gcggcattgt gaatcgctca ttgctgaatt
     1981 atgaggcggg tttacaactt aaacatcttg gtatggatat tgacccggac acgccgctga
     2041 aatatctctc cattggtcag tggcagatgg ttgaaatcgc caaggcgctg gcgcgtaacg
     2101 ctaaaattat cgcctttgat gagccaacca gctccctttc tgcccgcgaa atcgacaatc
     2161 ttttccgcgt tattcgtgaa ctgcgaaaag aggggcgggt gatcttatac gtttctcacc
     2221 gtatggaaga aatatttgcc ctcagcgatg ccatcactgt ctttaaagat ggacgttatg
     2281 tcaaaacctt taccgatatg cagcaggttg accacgacgc gctggtgcag gcgatggtcg
     2341 ggcgcgacat tggcgatatc tacggctggc aacctcgtag ttatggcgag gagcgcctgc
     2401 gtcttgatgc tgtgaaagca ccaggcgtgc gtacgccaat aagtctggcg gttcgcagtg
     2461 gtgaaattgt tgggctgttt ggtctggtag gggcggggcg tagcgaatta atgaaaggct
     2521 tgtttggcgg gacgcaaatc accgcaggtc aggtttatat cgaccaacag ccgatcgata
     2581 ttcgtaaacc gagccacgcc attgccgcag gcatgatgct ctgcccggaa gatcgcaaag
     2641 cggaaggcat tattcccgtg cactccgttc gcgacaatat caacatcagt gccagacgta
     2701 aacatgtgct cggcggttgt gtaatcaaca acggttggga agaaaacaat gccgatcacc
     2761 acattcgttc gctcaacatc aaaacgccgg gcgctgagca actgatcatg aatctctcag
     2821 gcggaaatca gcaaaaagcc attctgggcc gctggttatc ggaagagatg aaggtcattt
     2881 tgctggatga acctacgcgc ggcattgatg ttggtgctaa gcatgaaatt tacaacgtga
     2941 tttatgcgct ggcggcgcag ggtgtggcgg tgctgtttgc ctccagcgac ttacctgaag
     3001 tcctcggcgt tgccgaccgg attgtggtga tgcgggaagg tgaaatcgcc ggtgaattgt
     3061 tacacgagca ggcagatgag cgtcaggcac tgagccttgc gatgcctaaa gtcagccagg
     3121 cagttgcctg agtaaggaga gtatgatgtc ttctgtttct acatcggggt ctggcgcacc
     3181 taagtcgtca ttcagcttcg ggcgtatctg ggatcagtac ggcatgctgg tggtgtttgc
     3241 ggtgctcttt atcgcctgtg ccatttttgt cccaaatttt gccaccttca ttaatatgaa
     3301 agggttgggc ctggcaattt ccatgtcggg gatggtggcg tgtggcatgt tgttctgcct
     3361 tgcttccggt gactttgacc tttctgtcgc ctccgtaatt gcctgtgcgg gtgtcaccac
     3421 ggcggtggtt atcaacctga ctgaaagcct gtggattggc gtggcagcgg ggttgctgct
     3481 tggcattctc tgtggcctgg tcaatggctt tgttatcgcc aaactgaaaa taaatgctct
     3541 gatcacaaca ctggcaacga tgcagattgt tcgaggtctg gcgtacatca tttcagacgg
     3601 taaagcggtc ggtatcgaag atgaaagctt ctttgccctt ggttacgcta actggttcgg
     3661 tctgcctgcg ccaatctggc tcaccgtcgc gtgtctgatt atctttggtt tgttgctgaa
     3721 taaaaccacc tttggtcgta acaccctggc gattggcggg aacgaagagg ctgcgcgtct
     3781 ggcgggtgta ccggttgttc gcaccaaaat tattatcttt gttctctctg gcctggtatc
     3841 tgcgatagcc ggaattattc tggcttcacg tatgactagt gggcagccaa tgacgtcgat
     3901 tggttatgag cttattgtta tctccgcctg cgttttaggt ggcgtttctc tgaaaggtgg
     3961 catcggaaaa atctcatatg tggtggcggg tatcttaatt ttaggcaccg tggaaaacgc
     4021 catgaacctg cttaatattt ctcctttcgc gcagtacgtg gttcgcggct taatcctgct
     4081 ggcagcggtg atcttcgacc gttacaagca aaaagcgaaa cgcactgtct gatgcttttt
     4141 tccacaacaa tttaacgttt tttcccacca cagccagccg ccacaacggt tggctgttct
     4201 tcattgcaaa tggcgacccc cgtcacactg tctatactta catgtctgta aagcgcgttc
     4261 tgcgcaaaac aataagaaaa gagaaggagg agagccgggt gacagaaccg ttaaccgaaa
     4321 cccctgaact atccgcgaaa tatgcctggt tttttgatct tgatggaacg ctggcggaaa
     4381 tcaaaccgca tcccgatcag gtcgtcgtgc ctgacaatat tctgcaagga ctacagctac
     4441 tggcaaccgc aagtgatggt gcattggcat tgatatcagg gcgctcaatg gtggagcttg
     4501 acgcactggc aaaaccttat cgcttcccgt tagcgggcgt gcatggggcg gagcgccgtg
     4561 acatcaatgg taaaacacat atcgttcatc tgccggatgc gattgcgcgt gatattagcg
     4621 tgcaactgca tacagtcatc gctcagtatc ccggcgcgga gctggaggcg aaagggatgg
     4681 cttttgcgct gcattatcgt caggctccgc agcatgaaga cgcattaatg acattagcgc
     4741 aacgtattac tcagatctgg ccacaaatgg cgttacagca gggaaagtgt gttgtcgaga
     4801 tcaaaccgag aggtaccagt aaaggtgagg caattgcagc ttttatgcag gaagctccct
     4861 ttatcgggcg aacgcccgta tttctgggcg atgatttaac cgatgaatct ggcttcgcag
     4921 tcgttaaccg actgggcgga atgtcagtaa aaattggcac aggtgcaact caggcatcat
     4981 ggcgactggc gggtgtgccg gatgtctgga gctggcttga aatgataacc accgcattac
     5041 aacaaaaaag agaaaataac aggagtgatg actatgagtc gtttagtcgt agtatctaac
     5101 cggattgcac caccagacga gcacgccgcc agtgccggtg gccttgccgt tggcatactg
     5161 ggggcactga aagccgcagg cggactgtgg tttggctgga gtggtgaaac agggaatgag
     5221 gatcagccgc taaaaaaggt gaaaaaaggt aacattacgt gggcctcttt taacctcagc
     5281 gaacaggacc ttgacgaata ctacaaccaa ttctccaatg ccgttctctg gcccgctttt
     5341 cattatcggc tcgatctggt gcaatttcag cgtcctgcct gggacggcta tctacgcgta
     5401 aatgcgttgc tggcagataa attactgccg ctgttgcaag acgatgacat tatctggatc
     5461 cacgattatc acctgttgcc atttgcgcat gaattacgca aacggggagt gaataatcgc
     5521 attggtttct ttctgcatat tcctttcccg acaccggaaa tcttcaacgc gctgccgaca
     5581 tatgacacct tgcttgaaca gctttgtgat tatgatttgc tgggtttcca gacagaaaac
     5641 gatcgtctgg cgttcctgga ttgtctttct aacctgaccc gcgtcacgac acgtagcgca
     5701 aaaagccata cagcctgggg caaagcattt cgaacagaag tctacccgat cggcattgaa
     5761 ccgaaagaaa tagccaaaca ggctgccggg ccactgccgc caaaactggc gcaacttaaa
     5821 gcggaactga aaaacgtaca aaatatcttt tctgtcgaac ggctggatta ttccaaaggt
     5881 ttgccagagc gttttctcgc ctatgaagcg ttgctggaaa aatatccgca gcatcatggt
     5941 aaaattcgtt atacccagat tgcaccaacg tcgcgtggtg atgtgcaagc ctatcaggat
     6001 attcgtcatc agctcgaaaa tgaagctgga cgaattaatg gtaaatacgg gcaattaggc
     6061 tggacgccgc tttattattt gaatcagcat tttgaccgta aattactgat gaaaatattc
     6121 cgctactctg acgtgggctt agtgacgcca ctgcgtgacg ggatgaacct ggtagcaaaa
     6181 gagtatgttg ctgctcagga cccagccaac ccgggcgttc ttgttctttc gcaatttgcg
     6241 ggagcggcaa acgagttaac gtcggcgtta attgttaacc cctacgatcg tgacgaagtt
     6301 gcagctgcgc tggatcgtgc attgactatg tcgctggcgg aacgtatttc ccgtcatgca
     6361 gaaatgctgg acgttatcgt gaaaaacgat attaaccact ggcaggagtg cttcattagc
     6421 gacctaaagc agatagttcc gcgaagcgcg gaaagccagc agcgcgataa agttgctacc
     6481 tttccaaagc ttgcgtagga gcggttaatc tcccgtaagt ggaactaaca gcacatcgac
     6541 ctcacttgag gcaataactc ttttcgccga gcaggatgct cgcgaaaaga aactgtgatt
     6601 gtgattaccg caaatcacca aatcgaaatg atgtttgtga catacttcca gaatatgttc
     6661 gcttaactca ccgtaggcaa taaacgtctt gtcaacggga taccccgcat cctgaattaa
     6721 cttatcaaga aagctttgcg tttcttcttg catcacgctg cgtaaatctt caagcatcgg
     6781 cgcggctaat tgattgtaca tttccgggtc ggaagcgaga gtaattaaac tgatgtgtcc
     6841 ctttactggc ctggcgatag ataccgcttt tgccagcagt tgctgacttt ccggtgtaac
     6901 cgcaacagcg acaagaatat tgctatagct cataacctgt tccttattct gtgaacttca
     6961 ggtgacatta aagctttact cctaatggag aaacgacgca atcccaactc ggtcaaacag
     7021 gtaaatatta gtctgattat tagtaaagac aattaatcaa tcactcccgg tgatcatata
     7081 atttcaaatg taattataag aaaatagtgt actgattctc c
//
LOCUS       DAZGCS010000182         6893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGCS010000182 DAZGCS010000000
VERSION     DAZGCS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.185"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(281..766)
                     /gene="sixA"
                     /locus_tag="ACHK01_004678"
     CDS             complement(281..766)
                     /gene="sixA"
                     /locus_tag="ACHK01_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO8099479.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(969..3113)
                     /gene="fadJ"
                     /locus_tag="ACHK01_004679"
     CDS             complement(969..3113)
                     /gene="fadJ"
                     /locus_tag="ACHK01_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO8099480.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3113..4423)
                     /gene="fadI"
                     /locus_tag="ACHK01_004680"
     CDS             complement(3113..4423)
                     /gene="fadI"
                     /locus_tag="ACHK01_004680"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO8099481.1"
                     /translation="MGQVLPLVIRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4603..4887)
                     /locus_tag="ACHK01_004681"
     CDS             complement(4603..4887)
                     /locus_tag="ACHK01_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO8099482.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5259..6599
                     /gene="fadL"
                     /locus_tag="ACHK01_004682"
     CDS             5259..6599
                     /gene="fadL"
                     /locus_tag="ACHK01_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO8099483.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1724 a         1854 c         1625 g         1690 t
ORIGIN      
        1 atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt
       61 aataaatgac aatgagtgtc aaacagacat tattcatttc aacatgtaac tttgggcaaa
      121 atcaagatac gcgcagaaga ataatttccg aatgaatgta gaaagtgttt tatttataga
      181 taaggagagt cttcgatagt agtgaggttg acagatatcc gctgcctgat gagcaattat
      241 gcccaatgaa tctacctcat tgggcataat ttggaacaca tcagatagct tttgccatct
      301 tcagattaca cgggctcatc tgccagttga atgtaccgtt tccgctttca tcaagcgtta
      361 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
      421 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
      481 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
      541 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
      601 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
      661 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacg gaatcactgg
      721 cggcatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
      781 gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
      841 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
      901 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
      961 tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
     1021 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc aagtcgttgc
     1081 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
     1141 aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata
     1201 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
     1261 acctgcggtg cggagagtcg cccctgccct tgtgcgccaa tgagcgggta aatggcggga
     1321 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
     1381 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
     1441 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
     1501 tcatccaaaa gttggattgg acctaccgga aaaccaaatt tcactagcgc ggcatcaatg
     1561 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
     1621 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
     1681 ttcgccagtt ttaccgtagt ggcgatggtt tgcgctgatg tacccgcatg aggaataatc
     1741 tccaccagcg gcattttttc caccggactg aagaaatgca ggccgataac ttgctcaggt
     1801 cgcgtggcgt gagcggcgat atcaccaatc ggtaaagatg acgtattcga agcaaagatg
     1861 gtatgagcgg cgctattttg ctcaacttcc gccaccatct gttgtttcaa ttcgagattt
     1921 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
     1981 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
     2041 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
     2101 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
     2161 aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc actaccggga
     2221 tctttcttca cgtccgtact ggcaaaaaag atactacgca gcgcctgcga ttgtggcgtc
     2281 atcgccagtt cgccaaacgc ccgagcttcg gcgtcataac cgctgctggt gccctgcgct
     2341 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
     2401 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
     2461 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
     2521 gcgacttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
     2581 tgtttcgccc gaagttgttt tccggtgagg atcatctcta atgctgtgct gacgcctatc
     2641 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcaggc
     2701 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccaac
     2761 tccagcccac cacccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
     2821 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
     2881 ttgcagttgc cgatcatgtt gatgtctgcg ccagcaatga agttgtccgg tttagcggag
     2941 acaaacacca cgcctcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
     3001 tgcgaggcaa actccgcctt cagggtattc attttctcac ccggtacgtc gatggtgatg
     3061 atagcaatgt tgtccagacg aacattaaga gtaaacactg atgccatttc cattattccg
     3121 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
     3181 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg
     3241 cgaagggatg cccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
     3301 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
     3361 gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagatcgc
     3421 tcattgtcaa accggcacgt tccagcgcca gcggtgttga ccaggccgga ccgagcaaca
     3481 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
     3541 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgccgctgcg ccatcggtca
     3601 gcggcgtact gttcgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
     3661 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
     3721 tataaggagg gataaaggca gtcatcacct cttctttgag tttaccgtct gaccacgcct
     3781 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
     3841 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
     3901 ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcagtcgct
     3961 ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt ttactgacgc
     4021 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
     4081 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
     4141 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
     4201 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
     4261 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgcgggaa
     4321 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
     4381 tacgatcgcc ctggcggata accagcggta aaacctgacc cataacactc ccctgtaaaa
     4441 aaataaataa agtggtctga cctgatcata gtcttaacca tttttttaca tttagccaag
     4501 cggagaaaaa agaaagtggg agctatgaca cagtgaagac aggagaaaga aaagaaaacg
     4561 cccctgccag caactgacag gggcgaacgt acccgagagg aattaacgca gacccagctg
     4621 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
     4681 agactcttca gtgaaagtcg gggtgatttt gcacggttcg gattccacgc tacgggcttt
     4741 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg cttgcaaaca cgcggctgta
     4801 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
     4861 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
     4921 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
     4981 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
     5041 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
     5101 gaaaaaagat ggaaacatac ttgcaacatt ccagctggtc cgacctatac tctcgccact
     5161 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
     5221 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
     5281 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
     5341 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
     5401 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
     5461 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
     5521 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
     5581 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
     5641 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
     5701 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
     5761 gtcttggttt caacgccgtc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
     5821 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
     5881 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
     5941 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
     6001 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
     6061 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
     6121 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
     6181 ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc
     6241 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
     6301 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
     6361 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
     6421 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
     6481 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
     6541 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
     6601 aacgcgttcg cctggataaa gtcacctgca tagcaggtga ctttaactcc cccacttcac
     6661 cgaaagtagt gtcctcattg cttacctcct gagttttgca aacagcctgt tggcagcttg
     6721 ccctttcaaa tcagtaagtg gtattcacag acaaacctga agggaaatgc atattttcaa
     6781 gcgttctgct cgtccttctc aaagagttta cttttctgaa tttccaggat actccccccc
     6841 cccctggcta ttgtgcgctc atacactcaa attaaagata ggttctaaat aaa
//
LOCUS       DAZGCS010000183         6817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGCS010000183 DAZGCS010000000
VERSION     DAZGCS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.186"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHK01_004683"
     CDS             complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHK01_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO8099484.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHK01_004684"
     CDS             complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHK01_004684"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8099485.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHK01_004685"
     CDS             complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHK01_004685"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO8099486.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHK01_004686"
     CDS             complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHK01_004686"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5129..5131),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8099487.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHK01_004687"
     CDS             5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHK01_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO8099488.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1489 a         1844 c         1845 g         1639 t
ORIGIN      
        1 agcgcatcag gcaatcttac gcttgtcatc agtgacatac caataacctt atcgatgtgt
       61 tggagggaag aacgaggcaa acaggcactg gcggcatagc ttgaggtggt aacgcggaat
      121 acgggagatg gtgtcgtcac accctatggg ctgtaatccg ggcgacctgc gccacccgga
      181 aaacgtcaga gattactccc cttcacccgg gaacaggaac gggttgatgg aactgcgggc
      241 gtagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat ctgccacggc
      301 ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccgc agtcatcgca
      361 gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct
      421 ctgttcacag ttgctgcatt ttacgcgcac tacgtgccat tcggtttcac acaggttgca
      481 gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga
      541 accacaaacc gggcaatatt gacgttgctc gccgtattca gcgcgggctt tgccggggat
      601 cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg gcgctttatc
      661 gctgctgacg gatgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg
      721 agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt
      781 cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg
      841 aatatccagc gggggcttgc cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat
      901 ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg
      961 caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt
     1021 gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc
     1081 gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt
     1141 gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc
     1201 catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc
     1261 atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc
     1321 accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc gctcaccagc
     1381 agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc
     1441 ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata
     1501 tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca
     1561 aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt
     1621 tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg
     1681 aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg
     1741 tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat
     1801 ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggccgcaaag gtagccgcaa
     1861 agccaacagc cgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt
     1921 ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca
     1981 tgacgtgtgt accaccgacg cctgccggat cgtacagacc cgcattgtcg taaccgcggg
     2041 ttttcagctc agcaacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt
     2101 gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc accacgcggt
     2161 caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga
     2221 acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg aagtcgacga
     2281 taccgttggc atactgaata attgcacctt ccgccgggca cgctttcagg cagcctggat
     2341 cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgctcca
     2401 cttccgagaa gcgcattacc gtccacgatt tggcgcttaa atcattgggg ttgtcgtaca
     2461 ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac
     2521 aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct tcctggaagt
     2581 cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgag
     2641 attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa
     2701 actccggcgt ctgcgtgttc gcatcaccaa cgaatggcgt taacgtattg gcaataaagc
     2761 cttttttagc tacgccttca taaccccagt gaatcggaat accgatggta tcgatatctt
     2821 tgccgttcgc tttcagcgta cgaatacgtt tggtcaccac cgctttggct ttgatatagc
     2881 cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta ttcgccagcg
     2941 actccccgat ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg
     3001 tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg
     3061 ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc agccgggttc gagataacgt
     3121 ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg
     3181 gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc tgcatgataa
     3241 acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc
     3301 acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta ccctgcggat
     3361 ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat gcccagccca
     3421 gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg ttgccttccg
     3481 gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt
     3541 gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg
     3601 taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac ttcggctcat
     3661 gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg ccaccctgtt
     3721 cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca tcagtcagcg
     3781 caatccccgg ggcgtccgca cctttccagt gccactgcaa ccagcgacct gagttaacga
     3841 ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg gaacacttcg gtctggatct
     3901 tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag gtttcagtgt
     3961 tcagtgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc actttgtttt
     4021 tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg accttgccct
     4081 ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc ggcaaccagt
     4141 caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc atcatagaga
     4201 cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc ggttttggcg
     4261 tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac
     4321 ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg gagtgaccgc
     4381 gcagggcgtt aacgccgccg ccggccatcc ccatattgcc gagcagcagc tggatcatcg
     4441 ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg gcatacagga
     4501 acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact ttcaggaacg
     4561 cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct
     4621 gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc
     4681 cgttttcgtc cagttcataa gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt
     4741 agccggtgaa caggccatct tcaaagccgt aatcctcacg cactatcagg ctggcgttgg
     4801 tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc
     4861 ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca gctaccgccg
     4921 ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga attttggctt
     4981 ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc atcaccacga
     5041 cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg
     5101 gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca
     5161 tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag
     5221 cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg ttttgcgcaa
     5281 tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc
     5341 aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc
     5401 tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga
     5461 ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt tttgctccgt
     5521 caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg
     5581 tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagcgctacg ctgggtgcaa
     5641 aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc
     5701 ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg
     5761 cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc
     5821 agaattggct caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg
     5881 tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa
     5941 atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac
     6001 acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac aacggcattt
     6061 cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc
     6121 tttccgaggg gataatagaa agtccgcgcg atatcttcgg catggatgtc gttccttcct
     6181 gtaatggcct tgaagtacaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc
     6241 gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag caacttaatg
     6301 acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc aacaaactgg
     6361 atgatgcatt acgtcatctc aacgattttc agccagtggg gcaactgact ggttgtactc
     6421 acgccgctgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc
     6481 gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc
     6541 aaggtgcggt actggtttcc agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt
     6601 gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg
     6661 agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg gtttataccc
     6721 atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta aattgcagga
     6781 cttattcatt tcgtgaattt tattatttta tttataa
//
LOCUS       DAZGCS010000184         6727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGCS010000184 DAZGCS010000000
VERSION     DAZGCS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.187"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..302)
                     /locus_tag="ACHK01_004688"
     CDS             complement(<1..302)
                     /locus_tag="ACHK01_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8099489.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDV"
     gene            complement(312..638)
                     /locus_tag="ACHK01_004689"
     CDS             complement(312..638)
                     /locus_tag="ACHK01_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8099490.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(635..3057)
                     /locus_tag="ACHK01_004690"
                     /pseudo
     CDS             complement(635..3057)
                     /locus_tag="ACHK01_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(3003..3224)
                     /locus_tag="ACHK01_004691"
     CDS             complement(3003..3224)
                     /locus_tag="ACHK01_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099491.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(3269..5206)
                     /locus_tag="ACHK01_004692"
     CDS             complement(3269..5206)
                     /locus_tag="ACHK01_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8099492.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSCLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(5270..>6727)
                     /locus_tag="ACHK01_004693"
     CDS             complement(5270..>6727)
                     /locus_tag="ACHK01_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8099493.1"
                     /translation="FGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNWRQ
                     AAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVAA
                     DPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPA
                     GVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSVD
                     EAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDI
                     LQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWVHETAVVRRKSEASRFQ
                     DFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPD
                     ESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILITKQA
                     SGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT         1378 a         2214 c         1682 g         1453 t
ORIGIN      
        1 acatccggca cgggcagccc gacaacgttc aggatccggt cacgccatgg accactcagc
       61 acatgaagtc gtgacgctgc cgtgatttcc cggccggact gaccgcgcac ccagatgcgg
      121 atttccgcct gcgccatttc cgcaccggac tgcatccgct cccggctgct cctgccacgg
      181 atatccgcat gaattttccc gcatgacacc cattcttccg tcatttctcc ggcagcatta
      241 cgggttaaca ccgggttcag aacacttatc atctgtgtca gacgacctgc agatattgcc
      301 attccccctc ctcataacac cgtcggacaa cgcaaatcgt aaatcagcac ggacacagaa
      361 aacggcagtt ccccctgcac gaggtcttcc cgctcagcaa gatccggatt ccggtacagc
      421 atcccggtca gtcgcatggc agcccccttc atccgggtta atgcctcgcc cgggatcagc
      481 tcaccgtcct cacgaatcac cttatcccgg ctgccctgaa tgtaggccag cagcacggcg
      541 gtagcctgac gaaccttgtc catcagcatg tcatcatccg cgtcatggtc gacacgcaga
      601 tgtgccttga tctcttccag tgtcagtaat gccgtcattt tccgcctcct gcatcccgtc
      661 cacgtttggc agccagggtc cagcctgatg aatgagcttc tccgggttta tcaccggtca
      721 tactgttgca gtgccacagc gagccccccc acgtcaccgt atcaccgggg tggtaggttt
      781 caccggctct gaacacaccg cggtagagca tcaccggcag ggaaaatgtt ttttccgtac
      841 gctggccact gctctgccgg accaccacag tgaacgaccg ttcgccggtc atgctgacgt
      901 caatatcggc caccccgtca accaggcatt cccatccccg catcccgtgc gttttttcat
      961 acgcccgcca gagtccgccc tggtgtgtgg catacgtgcc ccggggaaag gatttttgat
     1021 cgtcaatggc ggggattatt tccagtgccg tggcatcacg cccgtcctgc ggagccggca
     1081 gggctctcac cgcatccaga acagccttct gcagaacatc gggatcatag tcacgaccat
     1141 cacgcggaac aggaatatgg cttaccgcct ctttcaccat ctgttcaagc atcggacgca
     1201 catcatcggg ggtaatactt ttgccgtccg ccggtaccgg aatattcgca accgcatcat
     1261 tcaccgcctg cttcagtact tccggatcat aatcacgacc gtcacgcgga gcagggatat
     1321 ggcttacagc ctctttcacc atctgctcaa gcatcggacg cacatcatcg ggggtgatac
     1381 ttttgccgtc cgccggtacc ggaatattcg caaccgcatc attcaccgcc tgctgcagta
     1441 catccggatc ataatcacga ccatcacgcg gtaccggaat ggtccccaca gcgtcatcca
     1501 ccatcgcctg cagaaccgga tgtacctcat ccaccgtcac atgcttctgt aataccgccg
     1561 acagggaagc cagtttctct tcaaacgctt gtgcctgcgc ggccatcttc ccctcaaatg
     1621 tgcgctgtaa atccgccagc accgtggaga attcttcgcc cagtgcacga ataatggaca
     1681 gttcccgttc cgtcattttc tcagtatccc cctgaacatc gctttcactg catcatgctc
     1741 tgtttcactg attgccttat taccgtcaga tgcgccgtca ggcagttgtg ctgaaactgt
     1801 tttcccggcc gacgcgaacg ggtcctcacg ggcatcacga cgggacagcg cctccagact
     1861 gtagttctgc tgctgaagat acagtgcatc accgccggca aggggcggca ggttctcacg
     1921 tttacgggcc tcattgggcg tgagaagcgt atttttcacc gattcaccca gtgttttcat
     1981 gcgccgttcg ctgtccattc tcagcagcgt ggtgacgtca aactccgtgc tctcgttttc
     2041 ccccgtttcc agcgcctcat ccagtaacag ctcaatggac tcaatcagcg tctgcagaca
     2101 ctgggaataa tactgctgct ccagcgcctc cacgttgtca ctggaaggcg ggtggccaac
     2161 gccaatcttg taggccggga cacggaacac cgaacagaca atttcagccg tcattttcag
     2221 ttgttccacc gtctgcgcat ccaccggtga aaacgtcgtg gggctgtatt ttgccccgtt
     2281 gctcagtatg gccgtcttcc cggcattttc gcccgtatat ccgctgtccc agttcccctt
     2341 cagttttttc gcgttttctt ccgtaatact gccggggacc tcaatcacgc ctgatggtcg
     2401 cccgccattt ctgaaaaaat acgtcgaatt tgcctgaata tgatgcccct gcatggcggc
     2461 cagtcctgcg gcatacaccg gcggcagccc cacaagcgga tgaaaaaaac agttaaaacg
     2521 gtcgtggatc acctcccggg caggcaccgt caccgactct gtgatcccac agttccggtc
     2581 cggcgtgatg cggtagaaca cctcgccgtc atccgccacc agaggttcaa cccggttcca
     2641 gtccagaata cgcagttctt tgatctgccc ccggggggtg cggattttca gcaccaccgt
     2701 attgccgtga cgcaatttgg aattcagcca cagttcaaaa aactggatgc gattctgctg
     2761 cgcattagga cgacgacaga gacgggcagt atctccctgc cgcttttccc ggcgtattcc
     2821 ctgtacatca gtctgcatca ggcgaagccg cattttggca atatcctggg atatcagcga
     2881 aatgcatgaa aacaccgcgt gaaaggacaa aacggtttcc ggatcggctt tcaccccctg
     2941 ctgccaggca ccggcaaaag gctcagccac cgcctgaaac agggaccgcc agcccacttc
     3001 tcttacgtca cgtcctgatt tctggttttt tcgggttcgc cgcaaaaggt tccacattcg
     3061 ccatgctccg catcacgttt ctttttctga cctgccggac gtcgcgctgt gatgtactcc
     3121 gccttcccca ggcgaaccag cacctccgca cacggctgtg ccacatcacg gatatccccg
     3181 gcccgggcat catgcgtgcc ctgcagatac tggatttttg ccatctgtta ctgcgggaag
     3241 ctcgcgcctc ccgccctcct catcagactc agccgccgga cgcactgccg tagttcactc
     3301 cggtgatcac cgccaccgcc gcggtacggc gacgacgcca gttgatccag cgctccgcac
     3361 ggatggccac gctgcctgtc tggaacatgg aaaccagctc caccggggac ggcgtggtac
     3421 tgtcgccggt cggctcagac tgcatttcca gtgatgcttc acgggacata tccactgcca
     3481 cgccgccgtc atccgccaga taaatatccg gggcattcac cagcaccagc tggtcaccca
     3541 cgtactggga gacaatcacc ggcagcccct ggaaggagcc acccagcagg gtcatgtccg
     3601 ggtattcctt ctgacccagc gcatttttac gcatggacag tgccagggca ttggtgctgg
     3661 acatcagcca gaccgcaccg gtgggctgca ggtttgctgc cacaaactgt ccaaacgcag
     3721 cctctgcatc cgcatccggg ttaccggttg atgccgtgcc cttcacatca tgggtgatgg
     3781 acgccgggga gacatctgcc actgcggctt ttttcgggtc cacaaagtct gtatccagac
     3841 gcgccaccac cgcttccgcc agcgcattac ggaccagtgc atcagcagcc ggactggaaa
     3901 aacggatcaa ttcttccgtc agtaccgcaa tggccgacac tttcgcatga ctgaaggtga
     3961 tggattcaaa atcaaacttc gtcaggggtc tggccttacc ctcacccacc cagccggcag
     4021 caccgccgga cacctgggcg tgcacacgga tattgaacgg cacctgacga agtgcaggga
     4081 tcccgccctg accaaatcgc ccgataatgg tctgcggacg caggtaatca ataaagtcct
     4141 gtgcgtattc ctgatattca gacaggctgc ctgcccactg cggatccgtg gtggtccccg
     4201 cgcccactgc cgatttcagg acatgatgca gacaactgtc atccggatac tgacgacggg
     4261 ccacttccag ggcttcagat cggacgcctt tagccgcagc cagcgatttg gcaaagcggg
     4321 cgaagccaat ccccttatcc agtttctgct ccacacggat caccggcgca gaagccaccg
     4381 cggccacatt cccgttaccg gcctgtttca ccggctgcgc cgtggcggcc ttaccggctt
     4441 ccagttcacg caggcgcttc aggtgcgcat ccacctgacg gatttccgct gcggtgttgt
     4501 cgtagtgctc ttcctcctcc acatccagcg tgcgcccttc ctctgcggct ttggtcatga
     4561 cctcctcaag ggaggctgcc agcgctgcac gcttgttttc aaaactttta atctgttcgc
     4621 caatattcat tatggtcttt tccttatgaa aaacggttgt tgactgtgcc gcagcgccgg
     4681 cagaagatgc gattttcacc accggtttcc ggttgccgga cgcggcagaa aacgggcggt
     4741 cgtaagattt aatggtccgg atggtgcatt ccgcattcgc gggcacggtg acggcagaca
     4801 cctccatcag ttcccagcgc agaaaatgca gtccgcctcc gtccagaaag gtgtattcat
     4861 ggggacggaa gcccacggac agccccctga ccagcccggt cttaatggcc gcccagacct
     4921 catccagccg ggcagccagt tgcgacggca tatccggtac gggcttcacc agtgttgccg
     4981 tgatttccag cccttcgctg acccggcgta ccgtacactg ccccaccggg cgggaatggt
     5041 catgctgcca gagaaacggg atcgcactgc caaactccgc gccctccggc tccaggatgt
     5101 caccatcccg atccggagaa ggcgttgacg caatcccggt gatcacccgt tcatcctcac
     5161 tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca gcctcctgaa
     5221 aataaaaaaa ccgccggagc ggttcgtgat ggttacagtg tgaacagggt tatatgaaaa
     5281 aaaccgcata ttctttcttt ttcggttccg ggttaaggga catcagggag accgcattga
     5341 acagcgccat cagcgggtca atttttcccc gtccgctggc ctgtttggtg ataagaatgg
     5401 cgttaccttt aggctccacc cgggcattac cgacacacca ggccatcagg ggctgatcac
     5461 cgtgaatcag caccccttca gccagtttgc gctcggtggt tttgatggcc ccgcccagct
     5521 tccagccctg gcttatcccc accacactct catcggggat cccggcttcc gccagtgaat
     5581 ccagaatctg ccccacccct gacgggtcaa taccgatatg atccagtaac tcagcctcat
     5641 gaatacgacg cacatactcc gccacttccg ccgtgtcatc cccgacccga cggacaatcg
     5701 tcatgtctcc acaggccaca aaatcctgaa accgggatgc ctcactcttc cgtctgacca
     5761 ccgcggtttc atgcacccag gcatggcccc agcccagcca ttcgcgggtt tccctgtcac
     5821 ggccagtcac gtacattccc agcagatcat ccaggccccc gccgtcaatc cccaccgtca
     5881 ccacatcagc gcgctgcagg atatcgtcca ggctgacgcg cctgccctgc tgctcccaga
     5941 aatccgcgcc cgcccagcgg tcagaacgca gggcaagacc gatttccaca ttggcgtgtt
     6001 ttgacatgaa gccacgaaat gcttcctcac cagcctcccg ggctttacgg tactcccggt
     6061 acagaaaggc ctcatccacc gaataaccga gattcgggtt aaccatggcg aggttttcca
     6121 tcagcaggtg agccccgctt tccaccattt caggaggatg ctcaaaaatc accggcagaa
     6181 agtgcggatc atgaattttg ccgtcgcgca catcccgggc atactgcagt ttctgtctga
     6241 acaccccggc gggcggttca ttcgactggg tggtcgtata caccacaaac ccttccggac
     6301 gggaggcaag cccgccgatg gcttcacgta gcatgtcttc cgccttgtac tgcttgccaa
     6361 acagccacag ttcatcaatc agagtcccca cggacttgat accggacacc gtattcggat
     6421 cggctgccac caccttcagg gtggtgtccg tcacccgatg ggtgatggtc cggatatgtg
     6481 tctgcacctg acagaggtca tccagatcat cgtcccgtcg taccatatcc ctggcagggt
     6541 tgaaggcgtt agccgccacc tccacggtcg gggccagaat ggtgtagccc gccgcctgcc
     6601 gccagttcag taacagcgcc gtcatcatga tccccgcagc cagcgtggac ttcgagtttt
     6661 tcttggggat aaggataaac acttccttga tatggcgaac accggtctgc gcatcgtagg
     6721 agccaaa
//
LOCUS       DAZGCS010000185         6712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGCS010000185 DAZGCS010000000
VERSION     DAZGCS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.188"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..396)
                     /gene="cspH"
                     /locus_tag="ACHK01_004694"
     CDS             complement(184..396)
                     /gene="cspH"
                     /locus_tag="ACHK01_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO8099494.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            682..894
                     /gene="cspG"
                     /locus_tag="ACHK01_004695"
     CDS             682..894
                     /gene="cspG"
                     /locus_tag="ACHK01_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO8099495.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            905..1093
                     /gene="ymcF"
                     /locus_tag="ACHK01_004696"
     CDS             905..1093
                     /gene="ymcF"
                     /locus_tag="ACHK01_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO8099496.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1068..1298
                     /gene="ymcE"
                     /locus_tag="ACHK01_004697"
     CDS             1068..1298
                     /gene="ymcE"
                     /locus_tag="ACHK01_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO8099497.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFREM
                     AYNISYLTRGPGTNTLLACCCLLRPEEVNSEY"
     gene            1288..1461
                     /gene="gnsA"
                     /locus_tag="ACHK01_004698"
     CDS             1288..1461
                     /gene="gnsA"
                     /locus_tag="ACHK01_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO8099498.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1510..2583)
                     /gene="yccM"
                     /locus_tag="ACHK01_004699"
     CDS             complement(1510..2583)
                     /gene="yccM"
                     /locus_tag="ACHK01_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8099499.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETANSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPTIGH"
     gene            complement(2655..5399)
                     /gene="torS"
                     /locus_tag="ACHK01_004700"
     CDS             complement(2655..5399)
                     /gene="torS"
                     /locus_tag="ACHK01_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO8099500.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPEPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLVQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANTDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMCSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            complement(5494..6567)
                     /locus_tag="ACHK01_004701"
     CDS             complement(5494..6567)
                     /locus_tag="ACHK01_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8099501.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYETILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     YDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIDK"
     gene            complement(6545..>6712)
                     /locus_tag="ACHK01_004702"
     CDS             complement(6545..>6712)
                     /locus_tag="ACHK01_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8099502.1"
                     /translation="LETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRPVTGSLL
                     KRIRNGKKAKS"
BASE COUNT         1633 a         1680 c         1596 g         1803 t
ORIGIN      
        1 gataatatag tttcaagcgg tactataact ggctgttaag tggcatttat aactattagt
       61 tgcagagata ttcggttaaa tggattagtt tcctgataaa aaagtaactt gcgttataat
      121 gcatggtgtt gactattgaa ctattccaat aaaaccagtc agctttaaac aagcagcgtc
      181 atattaagag agataaacat ttgccgctgt tggtcctcgc aggccattta cgcgacaaaa
      241 ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg cggaaatatg
      301 gacctggact tctttacgac cgtcggaggg gataatgaat cctttgccgc ttttgcgatc
      361 aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact gaaaatactg
      421 cgctgcacta tacggggtta ataaaataaa gccagcgaaa tttaagaccg ccggacggct
      481 aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg tttgaaagac
      541 agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta tccccttctt
      601 ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa agccctgttt gccgaaaggc
      661 ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg gtttagtaaa atggtttaac
      721 gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt tttcgtccat
      781 ttcaccgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa agttgaattt
      841 tctattgagc aggggcaacg tggccccgcg gcagcgaacg ttgttacgct ctaaggttgc
      901 cgttattact caacatctcc atttccgctg tccatgttgt catggttcac agtaccgcac
      961 atcggcattc gatgtgacgg agcgaaaccc tttgggcgct aagtgtattt tttgtaaatc
     1021 gacaatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg cgccgctgga
     1081 tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc ttatttttct
     1141 tcaacgctgt atgtatagta agcgataacc tgttgattat tgaatctttc agggagatgg
     1201 cttataacat ttcttacctg accaggggac cgggaaccaa caccttactg gcgtgttgct
     1261 gtcttttaag accagaagag gttaacagtg aatattgaag agttaaaaaa acaagccgaa
     1321 acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc tgaacaagaa tacagggaaa
     1381 gaagtctctg aaattcgctt caccgcacgg gaaaaaatga ccgggcttga aagttatgat
     1441 gtcaaaatca aaataatgtg attttgtgaa catcaccccg tgcgaggtga tgttccgctt
     1501 gttgctaatt tagtgaccaa tcgttggcgc ttgtggaatt aagcgtcggt acaattcctc
     1561 cggcaccggg ctttgccata ctcccgcata cattgcgtaa ccaatcaccg caaacataat
     1621 ccccagaacc agtagtgtca ttaaccagcc agacaacgca aaagcttttt tatttgccgc
     1681 aggtttttgc agtgaaaagg tcaatgttga ggctaccgga catgactcta cgcaagtcat
     1741 acagccggta cattccactg ttcgtacctg aattaattta tcgaccggga tccgtgatgg
     1801 gcaatttttt gcgcatttgc cacagtcgat acaactttcg gcattgcgac gaatcttaaa
     1861 cggcgataat agcgaaacca cgcccatcag cgcgccatat gggcaaagat aacgacacca
     1921 ggcatggcga ataaacaggc tggcaatcag caaaacggtc acgctgatta atgtcgcggt
     1981 ccccatatga cgaaagaaat cgagcatttt aacgtccatc accacgctgt agggcgacaa
     2041 cataaaatag tgaatcgcct gagcgggcat caataacgcg atatagagaa aaaaactcaa
     2101 cagcaaatac ttcacgccgc gcagaggaat atccagccag cggggaagga cacattgccg
     2161 accaaacagt ttgttaccga gatcgccgat taattcagaa agcgtaccaa ccgggcataa
     2221 ccatgagcaa aaggcctttt tgagtaatag actgatgacg ataaaagcga ccaataacag
     2281 catcgcggcg gcgtggacgg acggtaactg acctgtggta aggctatatt tcagattcat
     2341 cagaccggca atcggtagcc agccttcgat acctcccggt ctggcgacaa atgtcgtgct
     2401 atttgccgtt tcgtaatagc gcacccaata ccagaacgtg atggcaatat aaatattcat
     2461 tgccaacagt aataattgcg tcgctttacg ccaggtcgtg gcattgcgcc agttattcca
     2521 cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag cgggttcttt tattctctgc
     2581 catgattttg ccagtccgtt aagttgcata ccaaatgcca ctattctagt tgttcttaac
     2641 tggctgatat tgattcaaat cgcgttcagg tctttcttat gcaaccatgc ttccagagcg
     2701 gcaacactgc gtgtaatttc ttcgtgtgga aggggggcag ataatggctg ctgctccagt
     2761 tgtgcgcata gctggctggc actgcacatt cccagactcg agcaactgct ttttagctga
     2821 tgtgcggcac gctttatttt ctcgctatcc tgactggcgc gggcaatgtc gatttcatcg
     2881 agaagcggca gggcatgttg tgtaaataat accagccatt cgtggatctt ctccgtcccc
     2941 attaactgag catcttcatt gagttgcgat acatccagcg gttgatcggt attggcttgc
     3001 agttggagat agtgcgccag taactgaccg agcacttcac gcggcaccgg tttagggata
     3061 atcccgcgaa ataatgaact ggtacgctgg cgcaacgttt cgtcgatgac atgagcgcta
     3121 aagccaatca aaaccagcga cggatattgc tgtaccagtt gtcgggcaag ggtaatgccg
     3181 tcgatatccg gcagatcaaa atccaccagt gcggcagcaa acggttcgct attttgcagt
     3241 gtctctaaag cctgcgcggc attgccaaca gcaacaacct gcgcaccact ggtgttcagc
     3301 atctcaacgg taattcgctg ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg
     3361 tccagacgca ccgcctgatt ggctgttttt ggctcgggtg cagtggcaac acgtaacggc
     3421 aagcgtaaac aaaaacagct tccaacctcc ggcgtgctgg tggcgctcag ttcgccgccc
     3481 atcgcctggg ccagacggct actgatagtc agccccagcc cggtgccgcc gcgtttgccg
     3541 cttacctgca caaatggctg gaagatttct gccagtttcg cgggatcaat accgcagccg
     3601 ctgtcttcca cttcgaccag ccattgctcg ccatcagtgc ggctacgtaa aacgatctgc
     3661 ccttcgtcag taaaacgtag ggcgttgctc aacaggttgg ttataacctg acgaatacgt
     3721 cgcggatcgc ccattaacgc gctcggtaca tcatcggcaa ttgccgttgc caggcgaatc
     3781 gggcgacctt tcacccgtcc gctcattaat tgcagggtac tttccagcag cgggcgcggt
     3841 tcaaagggtt catcgctgac cgaaacattc ttgccgcctg cttcgatagc cgaataatcg
     3901 agaatatcgt tcaggatggt cagcaacgat tcaccagagt cagtaattgc ccgcaaatca
     3961 tcacgctggg cgttaagtgc ggggttatct gccagtagct gtgcggtgcc gagaataccg
     4021 tacagcggtg tgcggatctc atggctcatc gccgccagaa acgccgattt tgcctggctg
     4081 gctttttctg cttccgcccg tgcctggcgg tgttctatca ccagttcctg caattcagct
     4141 gtacgcgctt tgacctgcgc cgccagctgt tcgcggtggc gattcagtgc atgaacattg
     4201 ctgcgaaacg catccatcag ccgcccgatg gtatccagct cccgtacgcc agcggtttcc
     4261 gggaaagggg agtcaatatc accgtccagc agccgttgca gcgcctgcgt ttgttcggca
     4321 agtggacgcg tgactgagcg ataaaccacg cgccaaagga tcagaatcag tgcgcaaagt
     4381 gaaaccatcc ccagcaataa caggctgtat tgcccgcgtg cactggcttt ttccagatgc
     4441 gccagtccgt gctgattacg cagctcaatg gtgtcgacca gctgactgac ttcgctacta
     4501 aactgcgcga actgggcgat gttattttgt gcgagagttt gtaggtgatt gctgatttca
     4561 ctgtcctgct gatacagcgc cagcaaatcg ctatattggc taacggtagt taacgttgtt
     4621 gcgacctgcg cacgaacacc cggatcttca atgcgtattt gccgacgttg cagaattttc
     4681 accgcattat tgagctgctt ttccagcgtt ggtgcatttt tctggatctg ctccagcccc
     4741 agattcatca ccatttgctg cacccgcaga gcgctaaggc gcagttcatt catctggtta
     4801 acatactcaa gatcgatatc aatcagccga tcgagtgcac tttcagcagc ctgacgctga
     4861 tcttgttcga tcaaatcgta aatcccggcc tgggtcgctc cagcggaagt tgtcgcatta
     4921 ttcgcctgac cttgcgccag gcgtgcgatc tcatcggcgg cagcgactat ctgctgactg
     4981 agttgccgtt gttgctggcg tagttgcaga cgccgcccca ccagttcccc ttgctgacgt
     5041 aatgaacggg agatctcctg ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc
     5101 cgtaacgctt gcagcaacgc attaatcttc aggctttgtg cggtgagcat tcgcccctgc
     5161 gcctgccaca tcttttcgtt atcggcactg gtcaggttct gcgcggcgaa aagttcccag
     5221 gcgctggctt cgctcaactg gcgcgccata ttcatggtag gaatcaatgc ctgagtgttg
     5281 tctttttcca cctggctgat aaagcgcagg ttgtaccatc ccaccagggt actggtcagg
     5341 gttaacagcg ccatcagggc aaagcccatc cagagtcttc gggtcagggt taaattcacg
     5401 gtcggtgcac ttaaggtgaa taagttgtat gtttaaaatc tcttttacta tcaatgaatt
     5461 agtattggca ggtcattatt ggtcaaaata aaatcatttg tcgatttcaa ttttgtccca
     5521 ttcccgtcca cggctgtctc tataccgcgc cgccattgaa tctgatttat gcccgagaag
     5581 acgttgagca aatttatcgc caatctggtt ccggtatagc ctcgctgaca ggctacgcag
     5641 ttcatggaat gttggcgggt ttccatcaaa tgagagtcca gatgcatttc tcgcctttgt
     5701 aaaatacttt gatactgttt tcggggaaag aggatcgtaa tgctttgatg cgattatagt
     5761 ttcactgctg ctggcctccc tgcatttctg tagtgtatca gccaatgaga tattgagcgc
     5821 gtcaatcgtt agcgttagcg gaatggcgag tttagcccct gttttactct gttcaatgtg
     5881 aagatggttg tcgtttatgt ctgaccattt cattctgcac aaatcgccga ctctctgccc
     5941 tgtaacgacg gccaaatcca tcgccagcct tagccagata gggagaggtt cggctgcatg
     6001 gtaaatctcg acatactcat tagctgtcag ccttgagcgc cttacttctg actttgctgt
     6061 acgggttgct gttaccggat tcgttgccac atgcccctcg gctattgctt cacgaaaaac
     6121 gtcaacaagg gttgacctga ttaattttgc ggaagctgct ttaccttctg ctacgtaggt
     6181 gtttagcatt gctgccactt ctttcgttga tatgtcagtg agcggtttgt ccggcaattt
     6241 tcttcggatt gccctgattt tgctggcgta gtcgagtaga gttttcggcc tgatccccct
     6301 ttcggtgagg attgtttcat atcggtcaag ccacacatga agagtgattg cgtcaccgcc
     6361 tttaattctg tctattagtg atttgcgtcc gctgtctgag agtaactcaa tattggcctg
     6421 tattgcttca gtgattgcta tcctcctgtc tcggcctaaa ccaaactctt tacccgtcct
     6481 tgggtccctg tagcagtaat atccattgtt tcttatataa aggttagggg gtaaatcccg
     6541 gcgctcatga cttcgccttc ttcccatttc tgatcctctt caaaaggcta cctgttactg
     6601 gtcgatttaa gtcaaccttt accgctgatt cgtggaacag atactctctt ccatccttaa
     6661 ccggaggagg gaatatcctg cattcgcgca cccatcgacg aactgtttca ag
//
LOCUS       DAZGCS010000186         6658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGCS010000186 DAZGCS010000000
VERSION     DAZGCS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.189"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..325
                     /locus_tag="ACHK01_004703"
     CDS             161..325
                     /locus_tag="ACHK01_004703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099503.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRKHSTSPVEN
                     SLITNLSFVG"
     gene            complement(329..547)
                     /gene="ydfC"
                     /locus_tag="ACHK01_004704"
     CDS             complement(329..547)
                     /gene="ydfC"
                     /locus_tag="ACHK01_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO8099504.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMHQKINAAQDLKWANEVRAKQAE
                     NLSSLEQDITNLEKAAALTIARMITYPR"
     gene            complement(577..705)
                     /locus_tag="ACHK01_004705"
     CDS             complement(577..705)
                     /locus_tag="ACHK01_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099505.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLSSSAKSNG"
     gene            complement(707..862)
                     /locus_tag="ACHK01_004706"
     CDS             complement(707..862)
                     /locus_tag="ACHK01_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8099506.1"
                     /translation="MDTIDLGNSESLVCGVFPNQYGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            complement(1181..1657)
                     /gene="racR"
                     /locus_tag="ACHK01_004707"
                     /gene_synonym="rarC"
     CDS             complement(1181..1657)
                     /gene="racR"
                     /locus_tag="ACHK01_004707"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO8099507.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFILSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1782..2105
                     /locus_tag="ACHK01_004708"
     CDS             1782..2105
                     /locus_tag="ACHK01_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099508.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            2089..2514
                     /locus_tag="ACHK01_004709"
     CDS             2089..2514
                     /locus_tag="ACHK01_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099509.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            2537..3499
                     /locus_tag="ACHK01_004710"
     CDS             2537..3499
                     /locus_tag="ACHK01_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099510.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            3506..4246
                     /locus_tag="ACHK01_004711"
     CDS             3506..4246
                     /locus_tag="ACHK01_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8099511.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            4272..5042
                     /locus_tag="ACHK01_004712"
     CDS             4272..5042
                     /locus_tag="ACHK01_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8099512.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLLSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5058..5453
                     /locus_tag="ACHK01_004713"
     CDS             5058..5453
                     /locus_tag="ACHK01_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO8099513.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            5510..6094
                     /locus_tag="ACHK01_004714"
     CDS             5510..6094
                     /locus_tag="ACHK01_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO8099514.1"
                     /translation="MITFTKEQLISHVSENVKAMKFAVKQTVFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            6210..6314
                     /locus_tag="ACHK01_004715"
     CDS             6210..6314
                     /locus_tag="ACHK01_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099515.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            6560..>6658
                     /gene="hokD"
                     /locus_tag="ACHK01_004716"
     CDS             6560..>6658
                     /gene="hokD"
                     /locus_tag="ACHK01_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO8099516.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVR"
BASE COUNT         1705 a         1466 c         1827 g         1660 t
ORIGIN      
        1 ggcgttgcag cggggttgtc acttaagcgt atggtcaacc tgacaacccg gtgtcctcaa
       61 cgggggaagg aataaccccg ccatacttac cgccgcgcca tttcgcggat tgccacaacc
      121 ggaagcgcac ggtcgacgaa aatttaacga caggctatct atgaaccagc tacctcgccg
      181 tgcgctttcg cgttatggtc tgacttttca gggaaatatc ctttcagtaa actgtcagtg
      241 ccggatgctc acccgtgtcc ggcgcaagca ctccacctca cccgtggaga actccttaat
      301 taccaacctt agcttcgttg gttagctatt aacgcgggta tgtaatcatt ctggcaatgg
      361 ttaatgccgc tgctttttcc agattggtga tatcctgctc cagagaggac agattttcag
      421 cctgcttagc cctgacttca ttagcccatt tcagatcctg cgctgcatta attttctggt
      481 gcatccactc ataaagttca tcattggtat agtctggcgc gatgatgacg ggttctcgtt
      541 tctgcatact gattcctcgc ggtgctgttt cgcttatcag ccattagatt ttgccgaact
      601 ggaaagcacc tgtttaaatt cgttgaagct gtgagcttct tcgccttcgg caaggccttc
      661 gaagtattct tcgtaagcct tttccatgat tgtgtcgaaa tccatatcac ccacctgaat
      721 ttctttccag ccagcgacgc gctccagatt cggttttaaa cgttttgctt ttggtatacg
      781 tcattgcggt gaacgtaccg tactggttgg ggaacacgcc acataccaga gattcgctgt
      841 tgccaagatc gatagtatcc atgctgacct catttcccct taacgccggg gtagcggaac
      901 aaaaacctgc tgcatagtta ttaaagttga accctgccgt catgttctta cgcctcgggc
      961 tggctactta acccgtgacc actgcctggt aactcgaggt attgccctgt attgtgtggg
     1021 acgggatggg ttggtatggg aaaactatag gaaatgccta attacttgtc aataggctat
     1081 gcctaataat ttgggcgcaa cctaataggt gatggtttgt ggaagaggta gtaggagtta
     1141 actaacggga actaggaatt tcccgtcgga ccatataagt ttaagttcct gtcttggtga
     1201 tgttctggct tttccgtttt gattcttgat ttttcagata gttagctacc ttcatttcca
     1261 ttgcggcaat gtaggcgcga acgtcatgat caacccaact aggctccgta gcatttccag
     1321 ataagatgaa agccacaatt gctctttttt catcagaggc ggcttgataa aggctgttta
     1381 tgtctaaaag ttcacttttt gtatctgaag tggatggggt tggtatgggg tattcgttaa
     1441 gcccccaatg ctctggacca accacatcag aaaagaaacg ccatagttct ggaagtttgt
     1501 ctttacttat cgaacctttc ttaatccagt catggattga tggtggttgg actttgaaat
     1561 gacgtgcgat ttccgccttt gatttgacgg ctcctgatgc aatttttttg ttaatggcct
     1621 gctctatcgc tcggcctaag tctttaccac taagcattgc ttaatagtct cctatgcgca
     1681 tcgcgttagg caatccctac tctcgatgta ttaggcatag cctattgaca atttcattag
     1741 gctaagccta atattgttgc gtgttttttg gagttcattc gatgaaaaaa gataactatt
     1801 cattcaaacg agcttgtgct gttgtcggtg ggcaatcagc aatggctagg cttttaggtg
     1861 tatctcctcc aagcgtaaat caatggatca aaggtgtacg tcagttacct gctgagagat
     1921 gtcctgcgat tgaacgagca acaaaaggtg gtgttctgtg tgaagaactt cgtcctgatg
     1981 ttgattggac atacttacga cgctcgtcat gttattcgca gaatatgtcg atgaagcaac
     2041 caaatgacga aaacgatcat acccgaagca tcaagaggca aatgattcat gaaaatcaaa
     2101 catgagcaca tccgcatggc gatgaatgcc tgggcatacc ctgatggtga gaaagttcct
     2161 gcagctgaaa tagcccggac ttatttcgaa ctggggatga cgttccctga actgtacgac
     2221 gacagccatc cggaagccct ggcccgtaat acccagaaaa ttttccgttg gctggataaa
     2281 gacacccctg atgctgttga aaaaatgcag gctctgttac cggcgatcga aaaggcgatg
     2341 ccgcctttgc tggtggcccg tatgcgcagc cacagttctg aatattaccg tgagatcgtc
     2401 gaacggaggg atcggctggt gaaggatgtc gatgattttg ttgcgtcagc ggttgttttg
     2461 tatgaccaga tgaatcgcgg cggcccggca gggaatgctg tggtgatgca ctaaaagcac
     2521 ggtgttcggg ggttttatga gcagcaagct tcatggtctt gtctgggaag ggtgcgcctt
     2581 caccggcatg atcttatcca gggtggcggt tatggcccgt cttgcagact acagcaatga
     2641 cgagggcgtg tcatggcctg ccattgaaac tatccggcgt cagatcggtg caagaagtga
     2701 atccacagtg aaatcggcta ttgcagaact ggcgaaagag ggctggctga cgaaggaaga
     2761 gcgtaaggtc ggtgggcgta atgtaagcaa tatctatcgg cttaatgtgg aaaaactcga
     2821 agcagctgcg gcggcggcgc gtgagtcata taaaccgaaa agaaaaatta gcccggcaaa
     2881 aaatgacccg ttaacagttg acccgtcaaa tattgacccc tcaacggttg acccgtcaaa
     2941 ttttgatgga tcaactgttg ataaaaaact gccgattagg ggggcgatga ttgaccccga
     3001 tccgtcagta ttaaaacctg atccgtcaga taaaagatct tcttgtccgg acgcttcgca
     3061 accggacccg cagacggctg aacaggattt tttaacccga caccctgacg cggttgtgtt
     3121 cagtgcgaaa aaacgccagt ggggaagtca ggaagatttg gtgtgcgcac agtggatctg
     3181 gggacgaatc gtgagtcttt acgagcaggc ggccagcgat gatggcgaga tcactagacc
     3241 gaaagaaccc aactggacag catgggccaa tgacgttcgc acaatgcgga tgctggatgg
     3301 cagaactcac agacaaattt gtgaaatgtt tgggcgtctc cagcgggatt cgttctgggt
     3361 aaaaaacatc atgagtccgg caaaactccg ggaaaaatgg gatgaactgg ttatccgcct
     3421 ggggcgttcg cctgcgcagc gttgcgtgaa tcacatttct gaaccggaca ctgaaatacc
     3481 gccgggattc agggggtgac gtgtcatgaa aaacattgcg gcagttgggg ttcttgaacg
     3541 tattcgcaga cttgcaccac agggggcggt tccaccgtac cggacggtgg aggagtggcg
     3601 ggaatggcaa cttgctgaag gacgaaaacg cagcgaggag attaaccgcc tgaatcatca
     3661 ggtgcgggtt gaaaaaatcc tgaaccgtgc gggcatccag ccgcttcaca ggaagtgctc
     3721 attcgggaac taccgggtgc agaacgacgg tcagcgccat gctctgagcc aggcgaaatc
     3781 cattgccgat gaattgatga ccggatgtac aaacttcgtg ttcagcggta aacctggtac
     3841 cggtaaaaat cacctggcag cagcgattgg caatcggctg atggcgaagg ggagaagcgt
     3901 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gacggctacg acaacggcca
     3961 gtccggggaa aaatttttac aggagctttg tggagttgac cttctggtcc ttgatgaaat
     4021 tggcatgcag cgggatacgc gcaacgagca ggtcacgctg aaccagatag tcgaccgcag
     4081 aacggcttcg atgcgtagtg tcggaatgct gacgaacctg aatcacgcag cgatgagcac
     4141 actcctcgga gatcgggtga tggaccgtat gaccatgaat ggtggtcgtt gggtgaattt
     4201 taactgggag agctggcggt caaacgttgg acgtcagggt atgtgagaat ttttgacgag
     4261 gtaaattttc gatggaaact gtattgcatg cactgaaagc gatgggaaaa gccaattctg
     4321 ttgaactggc ggcgcggctt gatatcagcc gtgaagaagt tcttaacgaa ctgtgggaac
     4381 tcaaaaaaaa tggcgttgtt gataaaacgg gtcacacctg gtttctggct gtcgaaggtg
     4441 aagccggggt aaccgaaggg caggcactac aacctgaagc gccggatgtg gtaaccgaag
     4501 aggtcgctcc aaaagttacc gcagacatga tggttgagtt tatcggtcag gatggtgcta
     4561 aaacgtgtga ggaactggcg ggtaagttcg gcgtcagtac tcgcaaggtt gcttccacgc
     4621 tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt aaatttcgtt
     4681 actgcatgtc ggggggtaat ttaccagcag atccgaaagc cgcgccggta acgaaaaatg
     4741 atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa gccgcaacac
     4801 aggaagaaat taaaacagaa actgtggcgg acattgtgca gccgttgcta tcgtttaccg
     4861 aaacgcaagc agatgagctg atttttccgt ccctgcgcag ggcaaacctg gcgctgcgca
     4921 gggcgaaaag tgatgttcag aagtgggagc gagtctgcgc cgcgctgcgg gagctgaaca
     4981 agcaccggga tattgttcga cagattactg attcttcccg ccgtgttgta tcggaaaagt
     5041 gatagccgga ggcgcttatg gcgaaacctt ttacacacga acagcgtgaa gaactgaagg
     5101 cccgaattat cgggctggta cgcaaaaatg aacgcatgac gatatcacaa ctggagagag
     5161 cgacgggagc aggctggcat tcagtcagac gttgccttgt ggatgtactg gcttgtggcg
     5221 atttatacat gcccggtaaa tacggtgttt ttacatcaga acaggtgtat cgcgtatggc
     5281 gtaaggcagc ggagaaagca accgaccaga cattgattcg aaagttacca gacggagaaa
     5341 tacgccgcta cgacagacaa cagaacataa tctgtggcga gtgccggaag agtgaagtta
     5401 tgctgcgtgt actggcgttc tatcagggca attttcagga ggcggtactg tgagtgaatt
     5461 agctatcagg cttcaattgt cgctggcatt cgcatcaaag gagaatgaga tgatcacttt
     5521 tacaaaagag cagttaatca gtcatgttag tgaaaatgta aaggcgatga aatttgcagt
     5581 aaaacagaca gtattcaaaa attctctcga ggcaattgag ttggatttag cactggccct
     5641 tgttgctcag gcttcgctgg aagcagagcc cgtgctttat atgaatcgat ttaccggaaa
     5701 gacattctca ctggaagagc aacccggtgc tgataaggaa ccggaaatat acgtgccgct
     5761 atatgctgcc ccgccagaca gcgccgccat gcttcaggct ggaaactttc gggaaaataa
     5821 gggttcgtca accaataatt ttcgggaaat ctcggaaacg tcaaccaact atccggtaac
     5881 tccggatggt tggataagct gtagtgagcg aatgccggat gatggtcagc acgtaattat
     5941 tttatgtgat ggcgcattcg ttctttatgc gcaatatcga gacggtgagt tttttgatgt
     6001 agtccgtaat ggtgatgaat ttttcgaaac acagagccgc aatgtaaccg actggatgcc
     6061 gctaccagaa ccgccgcagg aggtgcgcca atgatctggc ctgaagcctt tgcaattaca
     6121 ggcgttgcta tagctattga ttttttagta tatgttattt gtcggtgggg gtaaaaacgt
     6181 tcgccgggat tcacaccaaa ggagggaata tgtcggatga tatttcactg gcaatggaag
     6241 gtgctctggc tgttattgct gttgtgggcg tttactgcct ggttgtgttt ttgatggatc
     6301 gactagggaa ctgaattcat tacgatatgg gaattcccat atcgggtaaa aacggtttgc
     6361 ggtaaagcga gagttaagta gaattgctgc gggtgcttga ggctgtctgc ctcgggcatg
     6421 ccaccgtaag gcagacagag aaaagcccca gttaacatta cgcgtcctgc aagacgccta
     6481 acattaatct gaggccaatt tcatgctaga catatgtagg ttagcctctt acgcgccgaa
     6541 aggcaaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat
     6601 ctgtatcacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacga
//
LOCUS       DAZGCS010000187         6588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGCS010000187 DAZGCS010000000
VERSION     DAZGCS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.190"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2917
                     /gene="ehxA"
                     /locus_tag="ACHK01_004717"
     CDS             <1..2917
                     /gene="ehxA"
                     /locus_tag="ACHK01_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO8099517.1"
                     /translation="VRSAGKKLILLIPDNYEAQGVGINELVKAADELGIEIHRTERDD
                     TAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIGGTAENVGNNLGKAG
                     TVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKSSIELINQLVDTVSSINST
                     VDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGSLGDGLDVVSGILSAVSASFILG
                     NSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTTAASAGLITSAVMLA
                     ISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKETGAIDAALTTINTVL
                     SSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFEHVAEKFAARINEWE
                     KEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQQHWDEKIGELAGIT
                     RNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLSTGNVSSVLTFITPT
                     FTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDYSKLIQFVEKNNKHY
                     QARIISELGDKDDVVYSGAGSSEVFAGEGHDTVSYNKTDVGKLTIDATGASKPGEYIV
                     SKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVIDNLHSVEELIGG
                     KHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQGDDQLFGGSGND
                     KLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGIDLLDGGEGNDYLNG
                     GFGNDIYVYRQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVGNDLIMNKAINGVLS
                     FNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKIPHNNNERSGYIKAS
                     NIASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLHQNNTHSTTLTTTV"
     gene            2967..5087
                     /gene="ehxB"
                     /locus_tag="ACHK01_004718"
                     /gene_synonym="hlyB"
     CDS             2967..5087
                     /gene="ehxB"
                     /locus_tag="ACHK01_004718"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO8099518.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5091..6530
                     /gene="ehxD"
                     /locus_tag="ACHK01_004719"
     CDS             5091..6530
                     /gene="ehxD"
                     /locus_tag="ACHK01_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO8099519.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2118 a         1026 c         1485 g         1959 t
ORIGIN      
        1 cgtacgttcc gctggcaaaa aactcatatt attaatacct gataattatg aagctcaggg
       61 cgtgggtatt aatgagttgg tcaaagctgc tgatgagctt ggaatagaaa tacaccgtac
      121 tgaacgagat gatacagcga ttgcaaacca gttttttggt gcagcagaaa aagttgtagg
      181 attaactgaa cgtggtgttg caatattcgc accacaactt gacaaacttc tgcagaagta
      241 tcagaaagtt gggagtaaaa taggaggaac cgctgaaaat gtaggtaata atctgggaaa
      301 agccggaaca gttctctcag cactacagaa ttttacgggg attgctttat caggcatggc
      361 tcttgatgaa ttgctgagaa aacaacggga aggagaggat ataagtcaga atgatattgc
      421 caaaagtagt attgaactta ttaatcagct tgtagataca gtatcaagta taaacagtac
      481 cgttgattca ttttctgagc agcttaacca gcttggctca tttttatcca gtaaacctcg
      541 cttaagttct gttggtggga aattacaaaa tttaccagac ctgggctccc tgggggatgg
      601 gctggatgtt gtctccggaa ttctttctgc tgtatcagca agctttattc tgggaaacag
      661 tgacgcacat acaggaacaa aagctgcagc gggtatagaa ctgacaactc aggttcttgg
      721 aaatgttggt aaagctgttt cgcaatatat tctggctcag agaatggcac aggggttatc
      781 gacaacagct gcaagtgcgg gtctgatcac atcggctgtt atgctggcta tcagtcctct
      841 ttctttcctg gctgctgcag ataaatttga gcgagctaag cagcttgaat catattctga
      901 acgatttaaa aaattgaatt atgaagggga tgctttactc gcagcctttc ataaagaaac
      961 cggagctata gatgcagccc tgacaacaat aaatactgtc ctgagttctg tatctgcggg
     1021 agttagtgca gcctccagtg catccctcat aggggccccg ataagcatgc tggtgagtgc
     1081 attaaccggt acgatatctg gcattctgga agcatcaaaa caggctatgt ttgagcacgt
     1141 tgcagagaaa ttcgctgctc ggatcaatga atgggaaaag gagcatggca aaaattattt
     1201 tgagaatggc tatgacgcaa gacatgctgc gtttttagaa gactctctgt ctttgcttgc
     1261 tgatttttct cgtcagcatg cagtagaaag agcagtcgca ataacccagc aacattggga
     1321 tgagaagatc ggtgaacttg caggcataac ccgtaatgct gatcgcagtc agagtggtaa
     1381 ggcatatatt aattatctgg aaaatggagg gcttttagag gctcaaccga aggagtttac
     1441 acaacaagtg tttgatcctc aaaaagggac catagacctt tcaacaggta atgtatcaag
     1501 tgttttgaca tttataacac caacatttac cccaggagaa gaagttagag aaagaaaaca
     1561 gagtggtaaa tatgaatata tgacatctct tattgtaaat ggtaaggata catggtctgt
     1621 aaaaggcata aaaaatcata aaggtgtata tgattattca aaattgattc agtttgttga
     1681 aaagaataac aaacactatc aggcgagaat aatttctgag ctcggagata aagacgatgt
     1741 ggtttattct ggagcaggct catcagaagt atttgctggt gaaggtcatg ataccgtatc
     1801 ttataataag acggatgttg gtaaactaac aattgatgca acaggagcat caaaacctgg
     1861 tgagtatata gtttcaaaaa atatgtatgg tgacgtgaag gtattgcagg aagtcgttaa
     1921 ggaacaggag gtgtcagtag ggaagcgaac agagaaaata caatatcgtg attttgaatt
     1981 cagaaccggt ggaattcctt atgatgtaat agataatctt cattctgttg aagagctcat
     2041 tggcggaaaa catgatgatg aattcaaagg cggtaagttt aatgatatat tccatggcgc
     2101 agatgggaac gattatatcg aaggtaatta tggtaatgat cgactatacg gcgatgatgg
     2161 ggatgattat atatccggag gacagggaga cgaccagtta tttggtggta gtggaaacga
     2221 taaattgagt ggaggggatg gtaataatta tctgacagga ggaagcggta atgatgagct
     2281 tcaggcacac ggagcttata atattctgtc aggtggtact ggtgatgata aactttatgg
     2341 tggtggtggt attgatcttc tggatggagg ggaaggtaat gactatctga atggtggttt
     2401 tggtaatgat atttatgttt ataggcaaaa ctatggtcat catacaattg cagatgaagg
     2461 aggtaaagga gatcgtttgc acttatctga tattagcttt gatgatatcg catttaagag
     2521 agttggaaat gatcttatca tgaataaagc cattaatggt gtactttcat ttaatgagtc
     2581 aaatgatgtc aatgggataa catttaaaaa ctggtttgcg aaagatgcct caggagcaga
     2641 taatcatctt gttgaggtta taacagataa agatggtcga gagataaaag ttgataaaat
     2701 acctcataat aataatgaac ggtcaggtta tataaaagcc agtaatatag catctgaaaa
     2761 aaacatggtt aatatcacca gtgttgccaa tgatattaat aagattattt cttcagtttc
     2821 aggattcgat tcaggtgatg aacgattagc atctttatat aatttatcct tacatcaaaa
     2881 caacacacac tcaacaactt taacgacaac tgtctgagac taaaagagga ggtgatggcc
     2941 attccaggag gaatggtcat cgttatatga tgagtaaatg tagttctcat aatagtctgt
     3001 atgcactgat attgcttgca caatatcata atataactgt caatgctgaa actataaggc
     3061 atcagtataa tacccacaca caagattttg gggtgactga atggttactg gcagcgaaat
     3121 ccattggctt aaaagcaaaa tatgtagaaa aacatttttc cagattgtca ataatttctt
     3181 tacctgcgtt gatatggcgg gatgacggta agcattatat attgtctcgt gttactaaag
     3241 attcatcacg ctatcttgtt tatgatccag aacaacatca gtcactaact tttagtcggg
     3301 atgagtttga aaaactgtat cagggaaaag tcattctggt tacgtcaaga gcaacagtag
     3361 tcggagagtt agctaaattt gatttttctt ggtttatccc ctctgttgtg aaatacagga
     3421 ggattttact tgaggtgtta actgtttctg cttttattca gtttcttgcg ttaataacac
     3481 ctcttttttt tcaggttgta atggataagg ttttagttca ccgggggttt tcaacgttaa
     3541 atattatcac aatagcattt attatagtga tactttttga agtgatatta accggagcca
     3601 gaacttatat tttctctcat actacaagtc gtattgacgt cgaactgagc gctaagttat
     3661 tcagacattt gcttgcattg cctgtttcat attttgaaaa tcgcagggtc ggagagaccg
     3721 ttgccagagt aagggaactg gagcaaattc gtaatttttt aaccggacaa gcgttgacat
     3781 cagttcttga tctatttttt tctgtaatat ttttttgtgt catgtggtat tacagccctc
     3841 aattaacact ggttatatta ttgtcactac cttgttatgt tatatggtca ttgtttatat
     3901 cacccttatt acgtcgacgt cttgatgata agtttctcag gaatgcagaa aatcaagctt
     3961 ttcttgtcga aacggtaaca gcaataaata caattaaatc catggcagta tcaccacaga
     4021 tgattgctac atgggataaa caactggccg ggtatgttgc ttccagtttc agggtaaatc
     4081 tggttgcaat gacagggcag caggggatac agctgataca gaaaagtgta atggtaatta
     4141 gtctatggat gggagcacat cttgtcatat cgggagagat aagcatcggg cagttaatcg
     4201 cgtttaatat gcttgcaggt caggtcattg ctcctgttat cagactggct catctttggc
     4261 aggattttca gcaggttggt atttctgttg agcgccttgg tgatgtatta aatacaccgg
     4321 tagagaaaaa gtcaggcaga aatatactgc cggaaattca gggggatatc gaatttaaaa
     4381 atgtcagatt tcggtattct tctgacggta atgttatttt gaataatatt aatttataca
     4441 tatcaaaggg ggatgttatc ggtatagttg gccgttctgg ttcaggaaaa agtacattaa
     4501 caaaactgct tcagcgcttt tatataccag agaccggaca gattttaatt gatgggcatg
     4561 atttatcact tgcagatcca gaatggttac gacgccagat tggtgttgta ttgcaggaaa
     4621 atatactatt aaatcgtagt attatcgata atattacatt agcttctccg gctgtatcta
     4681 tggaacaggc tattgaggca gccagacttg caggtgctca tgattttatt acagaactaa
     4741 aagaagggta caatactatt gttggagaac agggcgttgg tctttcagga gggcagcgcc
     4801 aacggatcgc aatagcccgg gctcttgtta caaatcctcg aattcttatt tttgatgaag
     4861 caaccagtgc tcttgattat gagtcggaaa atataataat gaaaaatatg tcaagaatat
     4921 gtaagaacag aaccgtaatt attattgcac acaggttgtc aactgtaaaa aatgcaaaca
     4981 gaataattgt tatggataat ggctttattt ctgaagatgg cacacataaa gagcttatct
     5041 ccaaaaaaga cagtttatat gcatatttat atcagttgca ggcataacgt atgcgctttt
     5101 acatgaaagg tttatgggat ttggtttgcc gttataagac ggttttttct gatgtctgga
     5161 aaattcgtca cacacttgat gctcctgtga gagaaaaaga tgagtatgca tttcttcctg
     5221 ctcaccttga actcattgaa acaccagtat ccagacgttc tcattttgtt gtatggagta
     5281 ttttattatt tgtaattata tctcttctct tatctgttct tgggaaagtt gaagtggttt
     5341 cagtagcaaa tggtaagttt actcatagtg gaagaagcaa agaaataaaa ccgatagaga
     5401 atgcgattgt agaaaaaata atggtaaaag atggctcttt tgtaaaaaaa aatgatccat
     5461 tagttgaatt gacggtgcct ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt
     5521 tgtatgaaaa aacagaacaa tatcgctacg caattctttc tgaatcaata caacggaatg
     5581 agcttcctga aataaggata acagattttc ctggcggaga agataatgcg ggaggtgaac
     5641 attttcagag ggttagctca ttaataaaag agcaattcat gacatggcag aacagaaaaa
     5701 atcaaaagca gctcacatta aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg
     5761 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa actcaatgat tttaagtatt
     5821 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
     5881 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
     5941 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
     6001 agcacagaaa atcaacagat aacattgtgt tgctggagca tgagcttgaa aaaaacaggc
     6061 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
     6121 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
     6181 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaacctg
     6241 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
     6301 ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacaggg cttgtattta
     6361 acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa atccctgtta
     6421 cagctggaat gactgttatg gcagaaataa aaaccggggt gcgtagtgtt atcagctatc
     6481 ttcttagtcc attaaaggaa actattaatg aaagtttacg tgaacgttag tatataaata
     6541 acatattggc tttttatatg atatagattt ctgaaatgat cttgtaaa
//
LOCUS       DAZGCS010000188         6575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGCS010000188 DAZGCS010000000
VERSION     DAZGCS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.191"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..200)
                     /locus_tag="ACHK01_004720"
     CDS             complement(<1..200)
                     /locus_tag="ACHK01_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO8099520.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKL"
     gene            complement(193..363)
                     /locus_tag="ACHK01_004721"
     CDS             complement(193..363)
                     /locus_tag="ACHK01_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO8099521.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(360..542)
                     /locus_tag="ACHK01_004722"
     CDS             complement(360..542)
                     /locus_tag="ACHK01_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO8099522.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            complement(539..1066)
                     /locus_tag="ACHK01_004723"
     CDS             complement(539..1066)
                     /locus_tag="ACHK01_004723"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO8099523.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAT"
     gene            complement(1063..1503)
                     /locus_tag="ACHK01_004724"
     CDS             complement(1063..1503)
                     /locus_tag="ACHK01_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO8099524.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(1577..1867)
                     /locus_tag="ACHK01_004725"
     CDS             complement(1577..1867)
                     /locus_tag="ACHK01_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO8099525.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(1864..2565)
                     /locus_tag="ACHK01_004726"
     CDS             complement(1864..2565)
                     /locus_tag="ACHK01_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8099526.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2562..3461)
                     /locus_tag="ACHK01_004727"
     CDS             complement(2562..3461)
                     /locus_tag="ACHK01_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8099527.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGNTKDTNTKENRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVDL"
     gene            complement(3494..3787)
                     /locus_tag="ACHK01_004728"
     CDS             complement(3494..3787)
                     /locus_tag="ACHK01_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO8099528.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(3907..4122)
                     /locus_tag="ACHK01_004729"
     CDS             complement(3907..4122)
                     /locus_tag="ACHK01_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099529.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            4226..4858
                     /locus_tag="ACHK01_004730"
     CDS             4226..4858
                     /locus_tag="ACHK01_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8099530.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            4948..5259
                     /locus_tag="ACHK01_004731"
     CDS             4948..5259
                     /locus_tag="ACHK01_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099531.1"
                     /translation="MNGDRYEFTKDSTEDSVFNVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVETWSITTDKKALYSKVMNIPGMQQLTSTKSFVGDVVGTCN
                     Q"
     gene            5479..5934
                     /locus_tag="ACHK01_004732"
     CDS             5479..5934
                     /locus_tag="ACHK01_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO8099532.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            5943..>6575
                     /locus_tag="ACHK01_004733"
     CDS             5943..>6575
                     /locus_tag="ACHK01_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099533.1"
                     /translation="MKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNAHGDG
                     DKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGDLIDR
                     SVDKDPDTNSFLLTNKEYLYGKFGINGWQKYVRFLRAFVDAYKNSDMIYTYLPGGDNC
                     TLSADNRIFIPVLGLGLINAVNEKDVIIVKQWRKYEGYRYLPCFDILKIQN"
BASE COUNT         1636 a         1589 c         1480 g         1870 t
ORIGIN      
        1 agtttatctt tctgtttctg ctcctctcgt cgtcgtttct tctctgctgc tttttccgct
       61 ttttcgcgtt ctttacttcg tcgttcgagt gctatcttgg ttccacactc tggagagcac
      121 caccactgat tagcgaatgc agggtgaaac cattcccgac attcatcgtt tttacatcgt
      181 cttcgcgctg gtttagccat catcttcttc ctcgtgcatc gagctattcg aatcgctcat
      241 cagctctgca cagcagtgct cacacacgtg aacttccagc acatgcagct tctgaccgca
      301 attagcgcac gttaaagccc gctcgacgct ttcttgttcg taacttcgat ttgggtcaat
      361 caccttgtat tcctcgcacg atgtcttagc caccggatat cccacaggtg agccgtgtag
      421 ttgaaggttt ttacgtcaga ttcttttggg attggcttgc gtttatttct ggagcgtttc
      481 gttggaaggt atttgcagtt ttcgcagatg atgtcggtga aacttcgtcg ttgtcgcctc
      541 atgtcgccct cctgacgccc tgcccgatcg ccatcaatgc cgctttggat acgatagtaa
      601 acatccgtcg aggactaatg aacggtcgcc aaatcagcag catggagcct ttgctgtttc
      661 ccttcttctc cagccctgtc gatggttcga taaaattaat ccgtccatca gtgataatac
      721 gaacttcgtc aacactctcc agagccttgc tgaaccatcc gacagacata tcctctggca
      781 caagcatcac taccgtctgt cgctgttgta tgcactgctc agcggctttt tccacccacg
      841 gcctgatatt gctgtacggt gggttattcc agattgcacc gtggcttatc cactcagaat
      901 ttagcgcgtc gtcagcctca gttagccagt gagcgcacag agcgtttttg tcgctcgctg
      961 ccgaatccag ccagaatcca aactcaatat ccagtgcatc aaaaagccaa agcggcgttt
     1021 gccagcagtc cttgtcgtgt gctggcgtat ttgatttgat agtcatgcag cccgatctcc
     1081 ccatcgcgct ttccattcga gagccagtcg cgcttcgtct gaccacttaa cgccacgctc
     1141 tgtaccgaat gcctgtataa gctctaatag ctccgcaaat tcgcttacac gcatcctgct
     1201 ggttggctgg cctattacca caaagccatt cccggcaagg ttaggaacaa catcctgctg
     1261 ctttaatgct gcggtaaaca cacacttcca gctttctgca tccagccagc gaccatgcca
     1321 ttcaacctga cgagagacgt cacctaagca ggcccatagc ttcctgtttt ggtctaagct
     1381 gcggttgcgt tcctgaatgg ttactacgat tggtttggtt gggtctggaa ggatttgctg
     1441 tactgcgtga atagcgtttt gctgatgtgc tggagatcga atttcaaagg ttagtttttt
     1501 catgacttcc ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttattagc
     1561 tcagtgatgt agatggtcat cttttaactc catataccgc caatacccgt ttcatcgcgg
     1621 cactctggcg acactcctta aaaatcaggt tcgtgctcat ctttccttcc cgttcttccc
     1681 tagtagcgaa ccggtaatac accgttcgcc agaccttacc ttcgataacc agaagacctg
     1741 cccgtgccat tttagccgcg gcctgattta tgctggttac tgttgcgcct gttaacgcgg
     1801 caacgtccgg cgcacagaag ctattatgcg tccccaggta atgaataatt gcctctttgc
     1861 ccgtcataca cttgctcctt tcagcccaaa cttagctttg atttctgcga tcttcgccag
     1921 agcctgtgca cgatttagag gtctaccgcc catgacagga agttgtttta ctggttcagg
     1981 tatagcctca ccacggttaa ttcgtgcggt catacaggac agttcatcgg cagccttgcg
     2041 ccgtaattcc gcatcagtaa gcgcattggc ccgcatgttc tgatacaggt tggtaaccag
     2101 ccagtagtgc gcgtttgatt tccacggata agactccgca tccggataca ggcctcgctt
     2161 ccggcaatac tcgtaaacca tatcaaccag ctcgctgacg tttggcagtc cggcgataac
     2221 ggatgcttct tcccggcacc atgcaacaaa ctgcccgggt gatggcagaa atggtcgatt
     2281 ctgccgacgg gctacgcgca ttcctgcgtt aacctgttcc attgtggtga tcccgttttc
     2341 ccggaaagcc agaacccact ggcggcggat ttcgttcagt tcgttctggt cacggttagc
     2401 caggctcgcc gggaaagttg ccagtaactg gctgaacaca ccgttgatga tctgcgctac
     2461 ctgctgtacc tgaggctttt cgtcgtactg ttccggcatg ttgttggcga tccgacgcat
     2521 ctgctcacgg tcaaagttaa ccatctgtgc ggcgatgttt ttcataaatc caccccgtaa
     2581 atccagtctg tgtttgtcag gtcgagtttt ggtttactgg ctgtcacgac tgcctgttgc
     2641 ttgttacggt tgatttcgag ctgggtccac ttgtcgcgga gtttggccgg gctcagcaca
     2701 ttaccggacc agaagttgtc ctggcaggcc cagcggaaca gcacacacat atcgcggtgg
     2761 ttacgtccgt cacgttcacg catcaggcgg atatcgttag cccaccctgc aaaattcggt
     2821 tttctggctg atggcgcgat agtcttcacc atgtcaaaca tccactctgc ggcggtcagg
     2881 tcttctgctg tcccccattt gctgccgctc tgaattgcag catcaggttt caccacagaa
     2941 aggtcgtttt ctggctggtc agaggattcg ccagaattct ctgacgaata atcttttcta
     3001 ttttcttttg tattagtgtc ttttgtgttc ccctgttttg agggatagca atcccccaat
     3061 ttgagggatg ttttatccct cgttttaggg gattttccct cgttttgagg gatacaccat
     3121 tctgagatgt ttttatttgg tccaaacatg ccgccttgct gcttgataat attcattctg
     3181 acgagttcta acttggcttc attgcaccgt ttgacaggta actttgtaat ctcgctaagt
     3241 tgagaatcgg tgattctgtc cattggttta ttccacccat aggttttacg cagaatggca
     3301 agcagcactt taaactgtcg cttggtcaga tctgcgcctg aataagcctc aagcagcata
     3361 tttgatagtc tggcgtaacc atcatcgaga tctgccacat tacgctcctg tccggcaaag
     3421 ttacctctgc cgaagttgag tatttttgct gtatttgtca taatgactcc tgttgatagt
     3481 tccagtaata acctcagaat tccatctgga tttgttcaga acgctcggtt gccgccgggc
     3541 gttttttatt ggtgagaatc gaagcaactt gtcgtgccaa tcgagccatg tcgtcgtcga
     3601 cgacacccca ttcaagaaca gcaagcagca ttgagaactt tggaatccaa tccctcttcc
     3661 acctgctgat ctgcgactta tcaactccca cagcttccgc tgtcttctca gttccaagca
     3721 ttgcgatttt gttaagcaac gcactctcga ttcgtagagc ctcgttgcgt ttgtttgcac
     3781 gaaccatatg taagtatttc cttagataac aattgattga atgtatgcaa ataaatgcat
     3841 acaccatagg tgtggtttat tttgatgccc tttttcaggg ctgggatgtg taagagcggg
     3901 aatgtcttaa gcggctttac cgcgtttagt tccgtactgt aaccaaaccg gatcacagtt
     3961 aagcgccata gcaatctcaa acaagaagcg cggtcgcttg gttactccag cttcaatcag
     4021 ttgaattgat tgctgtttaa caccggcttt ggttgccagt tcggtttgcg tcatttttaa
     4081 cgcaattcgc ctcttcttga ggcgttcaga aagagtttgc atatcgcctc catcaacaaa
     4141 ctttcttgta ttttcataca atgtatcttg tttgtcaaat acagtttttc ttgtgaagat
     4201 tgggggtaaa taacagaggt ggcttatgag tatttcttcc agggtaaaaa gcaaaagaat
     4261 tcagcttgga cttaaccagg ctgaacttgc tcaaaaggtg gggactaccc agcagtctat
     4321 agagcagctc gaaaacggta aaactaagcg accacgcttt ttaccagaac ttgcgtcagc
     4381 tcttggcgta agtgttgact ggctgctcaa tggcacctct gattcgaatg ttagatttgt
     4441 tgggcacgtt gagcccaaag ggaaatatcc attgattagc atggttagag ctggttcgtg
     4501 gtgtgaagct tgtgaaccct acgatatcaa ggacattgat gaatggtatg acagtgacgt
     4561 taacttatta ggcaatggat tctggctgaa ggttgaaggt gattccatga cctcacctgt
     4621 aggtcaaagc atccctgaag gtcatatggt gttagtagat actggacgcg agccagtgaa
     4681 tggaagcctt gttgtagcca aactgactga cgcgaacgaa gcaacattca agaaactggt
     4741 catagatggc ggtcagaagt acctgaaagg cctgaatcct tcatggccta tgactcctat
     4801 caacgggaac tgcaagatta tcggtgttgt cgtggaagcg agggtaaaat tcgtatgatc
     4861 aggattgcgg cgctactctc aatactctta actaccagcg ccaattctga atgctggatt
     4921 gtcacaaacc tgcacgggta cggggcaatg aatggcgatc gttacgagtt tacaaaagac
     4981 agcacggaag attccgtttt caacgtaaca ataaatggcg ataaatcatc agtttatgaa
     5041 tcagtttctg gcgtctatcc agagatgaaa tacactgctt tgtcatcgaa cactatggta
     5101 ggagaatacc agtctggagg aggcataacc gttgaaactt ggtcaatcac tacagacaaa
     5161 aaagctcttt actccaaagt aatgaacatc ccaggtatgc aacaacttac atcaaccaaa
     5221 tcctttgttg gtgatgtagt cggaacctgc aatcagtaat ccccacctca atctcgataa
     5281 caaaaaaaca aactattttc cgtttaaaaa caatggagtt tgtttttcac gccctttttt
     5341 acaatatttc ttgtttacaa catacaatct tccttgtaat tttaagccat cagcaggacg
     5401 cactgaccac cattgaaggt gaggctctta aaaattaagc cctgaagaag ggcagcattc
     5461 aaagcagaag gctttggtgt gtgtgatacg aaacgaagca ttggccggaa gtgcgaatcc
     5521 ggattagctg ccaatgtgcc attgcggggt gttttcgttc aggactacga ctcccacaca
     5581 caaccaaagc taactgacag gagaatccag atggatgcac aaacacgccg ccgcgaacgt
     5641 cgcgcagaga aacaggctca atggaaagca gcaaatcccc tgttggttgg ggtaagcgct
     5701 aaaccagtta accgccctat tctctcgctg aatcgcaaac cgaaatcacg agtagaaagc
     5761 gcactgaatc cgatagacct tacggtgctg gctgaatacc acgaacagat tgaaagcaac
     5821 ctgcaacgta ttgagcgcaa gaatcatcga gtttggtata gcaagccagg tgagttcggt
     5881 ataacttgtc aaggaagaca aaaggttaaa ggggaatcca ttccattggc atgaggtacg
     5941 taatgaagaa gattgattac aagtccatac caaaaccaat agactcagca tcagagcgaa
     6001 aaaaacacaa aaaagaggct gaaaaattag caaattatat cagttttatt agaaacaatg
     6061 ctcacggcga tggcgacaag aagttgcttt cagatgctcg gacccaagcg ttcggtatac
     6121 ttcgtaagca gatgcaatat cgtcttcatc ctggctacat aattgaaatt cgccctactg
     6181 aaagacaatt gttcttatta aactctgtct ttgactttgt aaacgtagtt ggagatctta
     6241 tcgacaggtc tgtagataag gatcctgata caaatagttt tcttctaaca aataaagaat
     6301 acttatatgg taaattcggt ataaacggat ggcaaaaata tgtacgcttc ttacgtgcat
     6361 tcgttgatgc gtataaaaat tccgacatga tttatacata tttgcctggt ggtgataatt
     6421 gtacgctttc ggcagacaat agaatattca tacctgtact tggtcttggg ctgattaatg
     6481 cagtaaatga aaaagatgtt ataattgtta agcagtggcg aaaatatgag ggatatagat
     6541 atctgccatg ctttgatata ttaaaaatac agaat
//
LOCUS       DAZGCS010000189         6475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGCS010000189 DAZGCS010000000
VERSION     DAZGCS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.192"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..777
                     /locus_tag="ACHK01_004734"
     CDS             <1..777
                     /locus_tag="ACHK01_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8099534.1"
                     /translation="VNPQMTLRRLPDEDPQNLADPAYRRRRIIMQNMRDEELAIAQVE
                     EMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEENNITQSGGTEWSKRDKSTYDPTDDIE
                     AYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGSNSELETAVKDLGKAVSYKG
                     MYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINAS
                     ARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
     gene            819..1217
                     /locus_tag="ACHK01_004735"
     CDS             819..1217
                     /locus_tag="ACHK01_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8099535.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1229..1582
                     /locus_tag="ACHK01_004736"
     CDS             1229..1582
                     /locus_tag="ACHK01_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8099536.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            1594..2172
                     /locus_tag="ACHK01_004737"
     CDS             1594..2172
                     /locus_tag="ACHK01_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099537.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2169..2564
                     /locus_tag="ACHK01_004738"
     CDS             2169..2564
                     /locus_tag="ACHK01_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8099538.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2572..3312
                     /locus_tag="ACHK01_004739"
     CDS             2572..3312
                     /locus_tag="ACHK01_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099539.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPSSTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            3328..3750
                     /locus_tag="ACHK01_004740"
     CDS             3328..3750
                     /locus_tag="ACHK01_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8099540.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            3732..4166
                     /locus_tag="ACHK01_004741"
     CDS             3732..4166
                     /locus_tag="ACHK01_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8099541.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4159..6475
                     /locus_tag="ACHK01_004742"
                     /pseudo
     CDS             4159..6475
                     /locus_tag="ACHK01_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1492 a         1626 c         2099 g         1258 t
ORIGIN      
        1 gtgaatccgc agatgaccct gcgtcgcctg ccggatgaag atccgcagaa tctggcggac
       61 ccggcttacc gccgccgtcg catcatcatg cagaacatgc gtgacgaaga gctggccatt
      121 gctcaggtcg aagagatgca ggcagtttct gccgtgctta agggcaaata caccatgacc
      181 ggtgaagcct tcgatccggt tgaggtggat atgggccgca gtgaggagaa taacatcacg
      241 cagtccggcg gcacggagtg gagcaagcgt gacaagtcca cgtatgaccc gaccgacgat
      301 atcgaagcct acgcgctgaa cgccagcggt gtggtgaata tcatcgtgtt cgatccgaaa
      361 ggctgggcgc tgttccgttc cttcaaagcc gtcaaggaga agctggatac ccgtcgtggc
      421 tctaattccg agctggagac agcggtgaaa gacctaggta aagcggtgtc ctataagggg
      481 atgtatggcg atgtggccat cgtcgtgtat tccggacagt acgtggaaaa cggcgtcaaa
      541 aagaacttcc tgccggacaa cacgatggtg ctggggaaca ctcaggcacg cggtctgcgc
      601 acctatggct gcattcagga tgcggacgca cagcgcgaag gcattaacgc ctctgcccgt
      661 tacccgaaaa actgggtgac caccggcgat ccggcgcgtg agttcaccat gattcagtca
      721 gcaccgctga tgctgctggc tgaccctgat gagttcgtgt ccgtacaact ggcgtaatca
      781 tggcccttcg gggccattgt ttctctgtgg aggagtccat gacgaaagat gaactgattg
      841 cccgtctccg ctcgctgggt gaacaactga accgtgatgt cagcctgacg gggacgaaag
      901 aagaactggc gctccgtgtg gcagagctgg aagaggagct tgatgacacg gatgaaactg
      961 ccggtcagga cacccctctc agccgggaaa atgtgctgac cggacatgaa aatgaggtgg
     1021 gatcagcgca gccggatacc gtgattctgg atacgtctga actggtcacg gtcgtggcac
     1081 tggtgaagct gcatactgat gcacttcacg ccacgcggga tgaacctgtg gcatttgtgc
     1141 tgccgggaac ggcgtttcgt gtctctgccg gtgtggcagc cgaaatgaca gagcgcggcc
     1201 tggccagaat gcaataacgg gaggcgctgt ggctgatttc gataacctgt tcgatgctgc
     1261 cattgcccgc gccgatgaaa cgatacgcgg gtacatggga acgtcagcca ccattacatc
     1321 cggtgagcag tcaggtgcgg tgatacgtgg tgtttttgat gaccctgaaa atatcagcta
     1381 tgccggacag ggcgtgcgcg ttgaaggctc cagcccgtcc ctgtttgtcc ggactgatga
     1441 ggtgcggcag ctgcggcgtg gagacacgct gaccatcggt gaggaaaatt tctgggtaga
     1501 tcgggtttcg ccggatgatg gcggaagttg tcatctctgg cttggacggg gcgtaccgcc
     1561 tgccgttaac cgtcgccgct gaaaggggga tgtatggcca taaaaggtct tgagcaggcc
     1621 gttgaaaacc tcagccgtat cagcaaaacg gcggtgcctg gtgccgccgc aatggccatt
     1681 aaccgcgttg cttcatccgc gatatcgcag tcggcgtcac aggttgcccg tgagacaaag
     1741 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt caaaaatccg
     1801 caggccagaa tcaaagttaa ccggggggat ttgcccgtaa tcaagctggg taatgcgcgg
     1861 gttgtccttt cgcgccgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc
     1921 ggcagcgtgc ttgtggtggg taaccgtcgt attcccggcg cgtttattca gcaactgaaa
     1981 aatggccggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
     2041 gtgaaaatcc cgatggcggt gccgctgacc acggcgttta aacaaaatat tgagcggata
     2101 cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc agcatcaact gaggatggta
     2161 ataaagcgat gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg
     2221 acaccggggc gacgtttttt gatggtcgcc ccgctgtttt tgatgaggcg gattttccgg
     2281 cagttgccgt ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct
     2341 ggcaggcgga gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg
     2401 atgcgtggat ggagtcccgg atttatccgg tgatgagcga tatcccggca ctgtcagatt
     2461 tgatcaccag tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt
     2521 ggagttcagc cgatctgact tatgtcatta cctatgaaat gtgaggacgc tatgcctgta
     2581 ccaaatccta caatgccggt gaaaggtgcc gggaccaccc tgtgggttta taaggggagc
     2641 ggtgaccctt atgcgaatcc gctttcagac gttgactggt cgcgtctggc aaaagttaaa
     2701 gacctgacgc ccggcgaact gaccgctgag tcctatgacg acagctatct cgatgatgaa
     2761 gatgcagact ggactgcgac cgggcagggg cagaaatctg ccggagatac cagcttcacg
     2821 ctggcgtgga tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggc
     2881 gatacccgtg cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgtggctgg
     2941 gtcagcagta tcggtaaggc ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc
     3001 accaatgtgg gacgtccgtc gatggcagaa gatcgcagca cggtaacagc ggcaaccggc
     3061 atgaccgtga cgccttccag cacctcggtg gtgaaagggc agagcaccac gctgaccgtg
     3121 gccttccagc cggagggcgt aaccgacaag agctttcgtg cggtgtctgc ggataaaaca
     3181 aaagccaccg tgtcggtcag tggtatgacc atcaccgtga acggcgttgc tgcaggcaag
     3241 gtcaacattc cggttgtatc cggtaatggt gagtttgctg cggttgcaga aattaccgtc
     3301 accgccagtt aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt tgaacataac
     3361 ggtgtgaccg tcacgctttc tgaactgtca gccctgcagc gcattgagca tctcgccctg
     3421 atgaaacggc aggcagaaca ggcggagtca gacagcaacc ggaagtttac tgtggaagac
     3481 gccatcagaa ccggcgcgtt tctggtggcg atgtccctgt ggcataacca tccgcagaag
     3541 acgcagatgc cgtccatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc
     3601 tggcccacgg aggcaatttc tcatgctgaa aacgtggtgt accggctgtc tggtatgtat
     3661 gagtttgtgg tgaataatgc ccctgaacag acagaggacg ccgggcccgc agagcctgtt
     3721 tctgcgggaa agtgttcgac ggtgagctga gttttgccct gaaactggcg cgtgagatgg
     3781 ggcgacccga ctggcgtgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc
     3841 accgctttta cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc
     3901 tgacgtacac cgtgctcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca
     3961 gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag
     4021 cggcagggct tgccggaggt gtccgctttg gcccggacgg gaatgaagtt atccccgctt
     4081 ccccggatgt ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga
     4141 tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttgagtctg
     4201 gatgcggcca gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa
     4261 agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcgc tgagccgaca ggcgctggct
     4321 gcacagaaag cggggatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgca
     4381 cagttcaccg acgtggccac gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg
     4441 caacaggggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg cctggtatca
     4501 gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca atcaggcggg
     4561 actgacggca gatcgtatgc tggtcctgtc cagagccggg caggcggcag ggctgacgtt
     4621 taaccagacc agcgagtcac tcagcgcact ggttaaggcg ggggtaagcg gtgaggctca
     4681 gattgcgtcc atcagccaga gtgtggcgcg tttctcctct gcatccggcg tggaggtgga
     4741 caaggtcgct gaagccttcg ggaagctgac cacagacccg acgtcggggc tgacggcgat
     4801 ggcacgccag ttccataacg tgacgacgga gcagattgcg tatgttgctc agttgcagcg
     4861 ttccggcgat gaagccgggg cattgcaggc ggcgaacgag gccgcaacga aagggtttga
     4921 tgaccagacc cgccgcctga aagagaacat gggcacgctg gagacctggg cagacaggac
     4981 tgcgcgggca ttcaaatcca tgtgggatgc ggtgctggat atcggtcgtc ctgataccgc
     5041 gcaggagatg ctgattaagg cagaggctgc gtttaagaaa gcagacgaca tctggaatct
     5101 gcgcaaggat gattattttg ttaacgatga agcgcgggcg cgttactggg atgatcgtga
     5161 aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct gagcagcaga ctcaacagga
     5221 caaaaatgcg cagcagcaga gcgataccga agcgtcacgg ctgaaatata ccgaagaggc
     5281 gcagaaggct tacgaacggc tgcagacgcc gctggagaaa tataccgccc gtcaggaaga
     5341 actgaacaag gcactgaaag acgggaaaat cctgcaggcg gattacaaca cgctgatggc
     5401 ggcggcgaaa aaggattatg aagcgacgct gaaaaagccg aaacagtccg gcgtgaaggt
     5461 gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc ttcaggcaga
     5521 actccggacg ctggagaagc atgccggagc aaatgagaaa atcagccagc agcgccggga
     5581 tttgtggaag gcggagagtc agttcgcggt actggaggag gcggcgcaac gtcgccagct
     5641 gtctgcacag gagaaatccc tgctggcgca taaagatgag acgctggagt acaaacgcca
     5701 gctggctgca cttggcgaca aggttacgta tcaggagcgc ctgaacgcgc tggcgcagca
     5761 ggcggataaa ttcgcacagc agcaacgggc aaaacgggcc gccattgatg cgaaaagccg
     5821 ggggctgact gaccggcagg cagaacggga agccacggaa cagcgcctga aggaacagta
     5881 tggcgataat ccgctggcgc tgaataacgt catgtcagag cagaaaaaga cctgggcggc
     5941 tgaagaccag cttcgcggga actggatggc aggcctgaag tccggctgga gtgagtggga
     6001 agagagcgcc acggacagta tgtcgcaggt aaaaagtgca gccacgcaga cctttgatgg
     6061 tattgcacag aatatggcgg cgatgctgac cggcagtgag cagaactggc gcagcttcac
     6121 ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg tgggattgtc
     6181 gggagtatcg gcagcgccat tggcggggct gttggtggcg gcgcatccgc gtcaggcggt
     6241 acagccattc aggccgctgc ggcgaaattc cattttgcaa ccggaggatt tacgggaacc
     6301 ggcggcaaat atgagccagc ggggattgtt caccgtggtg agtttgtctt cacgaaggag
     6361 gcaaccagcc ggattggcgt ggggaatctt taccggctga tgcgcggcta tgccaccggc
     6421 ggttatgtcg gtacaccggg cagcatggca gacagccggt cgcaggcgtc cggga
//
LOCUS       DAZGCS010000190         6150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGCS010000190 DAZGCS010000000
VERSION     DAZGCS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.193"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..764)
                     /locus_tag="ACHK01_004743"
     CDS             complement(<1..764)
                     /locus_tag="ACHK01_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8099542.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGIL"
     gene            complement(887..1339)
                     /gene="tabA"
                     /locus_tag="ACHK01_004744"
     CDS             complement(887..1339)
                     /gene="tabA"
                     /locus_tag="ACHK01_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO8099543.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1484..2077)
                     /locus_tag="ACHK01_004745"
     CDS             complement(1484..2077)
                     /locus_tag="ACHK01_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8099544.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2148..2861
                     /gene="bdcA"
                     /locus_tag="ACHK01_004746"
     CDS             2148..2861
                     /gene="bdcA"
                     /locus_tag="ACHK01_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8099545.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2992..3387
                     /locus_tag="ACHK01_004747"
     CDS             2992..3387
                     /locus_tag="ACHK01_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8099546.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3668..3802
                     /gene="pyrL"
                     /locus_tag="ACHK01_004748"
     CDS             3668..3802
                     /gene="pyrL"
                     /locus_tag="ACHK01_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO8099547.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3806..4741
                     /gene="pyrB"
                     /locus_tag="ACHK01_004749"
     CDS             3806..4741
                     /gene="pyrB"
                     /locus_tag="ACHK01_004749"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO8099548.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4754..5215
                     /gene="pyrI"
                     /locus_tag="ACHK01_004750"
     CDS             4754..5215
                     /gene="pyrI"
                     /locus_tag="ACHK01_004750"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO8099549.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5288..5674
                     /gene="ridA"
                     /locus_tag="ACHK01_004751"
     CDS             5288..5674
                     /gene="ridA"
                     /locus_tag="ACHK01_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO8099550.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            5740..>6150
                     /locus_tag="ACHK01_004752"
     CDS             5740..>6150
                     /locus_tag="ACHK01_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8099551.1"
                     /translation="MAKPRFTNEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFICF"
BASE COUNT         1642 a         1494 c         1401 g         1613 t
ORIGIN      
        1 ttcagtatcc ctttttcacc aagcaagtat gaaatcgatt cattacatac ataaggcatt
       61 aatagcagat gttctttcct tattgctaat aatgcattat tcaatggttt ttctaataaa
      121 tccgtaatcg tagaatcaac tgaatcttta caactcacct gcgagttatt caagtcctga
      181 gaattattta actcctgcgg aatatttaat agacgagcta tttccttgtt agcttcgcgt
      241 tggtaacacg ccagaatctt gttagtaatc aggtgtaatg aactctcatc ttttctttca
      301 aaggacttcc cttcataacg attagtcgaa ccatcaggtt gtgtaaatat aatgactgta
      361 tgtccttcac gaatcccatt attaaaagct gaggcaagtg taatttctat ttccacctta
      421 gataaatccg ctcgctcccc ttcaggatta ttcataacaa acttcattat ctcactacca
      481 tcattatttt tgatataaga tgaggactta atatgagcca tcatatttct acccgcttcg
      541 ttggagcgtt tgctaaaatc cacataaccc agggtaaata tcttagccaa aaactccaaa
      601 aatgctctgg gagaaagaat attgcttctg ctattctgcc ctccggcaat tcgggcaaaa
      661 tcattcattc ccacatacca ggtatctaag tcttcatttt tttgtttaca attgtcggct
      721 gatgtaatgt tttgagaaaa attccaacct gatattttgc tcatgacgga ctccttgagt
      781 taataagaac gtctaagcat tacaaatttt actcacaaaa aaatatcaaa aaacgctctt
      841 ttcacaaact cgccgtaaat ctggagttat ttacggcgaa aagtcatcac gccatcagca
      901 tcttcactac tgctttgcga acctgggctg gtgcacccac tgcgcacagc ggtttatgca
      961 cttcccccgg ataaaacaca acaaaatcac cttcattaag gataactgtt ttctcatcaa
     1021 cgccttccgg caaaaatgcg atgtctttat cagctaacca gtcggtatcc ggcgtgcctg
     1081 caggttgcgt gctgaaggtc atgccttcct gaccttttaa cacaatctga atgtcgagat
     1141 agcgggcatg gtactccgca cggcgggctt cgtacggctc ggtcatatct tccgagataa
     1201 gataaaacag acgattgcct tcgatatcgt gcttgccctt tggcgtttct gccgtaacgt
     1261 gtgctttgat atgctcaatc gcctggcgta actcctgggg tagccacggc tgaagattat
     1321 gaatatttcc gatgatcatc ttttccactc cttaattaaa acaacgtttc atttctatcg
     1381 ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt gaattattgt cttatcacaa
     1441 cttatcggca ggcgcgttcc cgcctttcgg atcaaggcca gcatcactcc ttgagaagag
     1501 tttttatcgc ttcccccgcc agtgcagctg ttgcacagag ttgttcgatt gagtgcccct
     1561 cccgtgcctt cgcagaaagc cctgacatca cggtactcat aaaatcagtc acacattgtg
     1621 cgctttgtgg atgccgcctg gcgatatagt cataaatggt cgtttctgcg gcgtgataat
     1681 attgaacggc aatatcacgc gctagtggat catgactatg aataccttca agaaccatac
     1741 agccagcgca gccgccgttt tggctatatc tgcgcgccgc ttcttttaat acctcaacca
     1801 ggcactcgcc tactggacga tcatcacgaa gaatatcggc aagcggaata gcttccgtac
     1861 cgacgtattc attgagtaca cggctaaata acccagcttt actgccaaaa gccgcgtaga
     1921 ggctcggggg gttaatacca agataatcag taacttcagc aacactgaca gcatcgaaac
     1981 ctttttggtg aaaaagcact tttgccgcag agattgcctg ctcaggagcg aaacgtctgg
     2041 gacgacctgg aacgcgagat tgttttttag tgaccattac aaaacctgtt gacagaaagt
     2101 taaaacagtt ttgtaatgca tgttacataa taaatcaagg agtccttatg ggcgctttta
     2161 caggtaagac agttctcatc ctcggtggca gtcgtggtat cggtgccgct atcgtacgtc
     2221 gtttcgtcac cgatggggcc aatgtacgat tcacctatgc ggggtcgaaa gatgccgcta
     2281 aacgcctggc acaagagact ggagcgacag cagtattcac agatagtgct gacagagacg
     2341 ctgtcattga tgtcgttcgt aagagcggcg cattggatat cctggtggta aatgcaggta
     2401 ttggcgtctt tggcgaggcc ctggaattaa atgccgacga tattgatcgc cttttcaaaa
     2461 tcaatattca tgctccttat catgcctctg ttgaagccgc ccggcagatg cccgaaggcg
     2521 ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat gcctgttgca ggcatggctg
     2581 cttatgccgc cagcaaatct gccctgcaag gcatggcgcg cgggctggcc cgtgattttg
     2641 gaccgcgtgg gatcaccatt aacgtcgtcc agccagggcc aattgatacc gacgctaatc
     2701 ccgccaacgg gccaatgcgc gatatgttgc atagtttgat ggctatcaaa agacatgggc
     2761 aaccggaaga ggtcgctggt atggtcgcat ggttagcagg gccagaagcc agttttgtta
     2821 ccggcgcgat gcataccatt gatggcgcgt ttggcgcata accgactacg ctcaattaag
     2881 cccagccatt atccatgatg tctgggtttt gtttactcac gtcgtccgct aaaagcggct
     2941 cccggtaaag agaaatcttc tgttcatgtc gtaaatcttg aggataacca aatggtagaa
     3001 agaaccgctg ttttccctgc tggccgacat tcactatatg ctgagcatcg ttattctgcg
     3061 gctattcgtt ccggcgattt gctgtttgtt tccgggcaag taggaagtcg agaggacgga
     3121 acaccagaac ccgattttca gcaacaagtc agactggcat ttgataattt gcatgcgacc
     3181 ctggcagctg cgggatgcac ttttgacgat atcattgatg ttacgagctt ccataccgat
     3241 ccagaaaacc aatttgaaga catcatgacg gtgaaaaatg aaatatttag cgccccacct
     3301 tatccaaact ggacggcggt gggtgttaca tggctggcag gctttgattt tgaaattaaa
     3361 gtgatagcgc gcatccctga gcagtaagca atagtgttag ccgttcgctt tcacactccg
     3421 ccctataagt cggatgaatg gaataaaatg catatctgat tgcgtgaaag tgaaaaagaa
     3481 aaaagcaggg aatgtctgca attattgata ccgaacgaca gttcccctgc agaatcacat
     3541 caaataaaaa tgcatatacc ttgactttta attcaaataa accgtttgcg ctgacaaaat
     3601 attgcatcaa atgcttgcgc cgcttctgac gatgagtata atgccggaca atttgccggg
     3661 aggatgtatg gttcagtgtg ttcgacattt tgtcttaccg cgtctgaaaa aagacgctgg
     3721 cctgccgttt ttcttcccgt tgatcaccca ttcccagccc ctcaatcgag gggctttttt
     3781 ttgcccaggc gtcaggagat aaaagatggc taatccgcta tatcagaaac atatcatttc
     3841 cataaacgac cttagtcgcg atgaccttaa tctggtgctg gcgacagcgg cgaaactgaa
     3901 agcaaacccg caaccagagc tgttgaagca caaagtcatt gccagctgtt tcttcgaagc
     3961 ctctacccgt acccgcctct ctttcgaaac atctatgcac cgcctgggtg ccagcgtggt
     4021 gggcttctcc gacagcgcca atacatcact gggtaaaaag ggcgaaacgc tggccgatac
     4081 catttcggtt atcagcactt acgtcgatgc gatagtgatg cgtcatccgc aggaaggtgc
     4141 ggcgcgcctg gccaccgagt tttcaggcaa tgtaccggta ctgaatgccg gtgatggctc
     4201 caaccaacat ccgacgcaaa ccttgctgga cttattcact attcaggaaa cccaggggcg
     4261 tctggacaat ctccacgtcg caatggttgg tgacctgaaa tatggccgca ccgttcactc
     4321 cctgactcag gcgttagcga agttcgacgg caaccgtttt tacttcatcg cgccggacgc
     4381 gctggcaatg ccgcaataca ttctggatat gctcgatgaa aaagggatcg catggagtct
     4441 gcacagctct attgaagaag tgatggcgga agtagacatc ctgtacatga cccgcgtgca
     4501 aaaagagcgt ctggacccgt ccgagtacgc caacgtgaaa gcgcagtttg ttcttcgcgc
     4561 cagcgatctc cacaacgcca aagccaatat gaaagtgctg catccgctgc cgcgtgttga
     4621 tgagattgcg acggatgttg ataaaacgcc acacgcctgg tacttccagc aggcaggcaa
     4681 cgggattttc gctcgccagg cgttactggc actggttctg aatcgcgatc tggtactgta
     4741 aggggaaata gagatgacac acgataataa attgcaggtt gaagctatta aacgcggcac
     4801 ggtaattgac catatccccg cccagatcgg ttttaagctg ttgagtctgt tcaagctgac
     4861 cgaaacggat cagcgcatca ccatcggtct gaacttgcct tctggcgaga tgggccgcaa
     4921 agatctgatc aaaatcgaaa ataccttttt gagtgaagat caggtagatc aactggcatt
     4981 gtatgcgccg caagccacgg ttaaccgtat cgacaactat gaagtggtgg gtaaatcgcg
     5041 ccccagtctg ccagagcgca tcgacaatgt gctggtctgc ccgaacagca actgtatcag
     5101 ccatgccgaa ccggtttctt ccagctttgc cgtgcgaaaa cgcgccaatg acatcgcgct
     5161 caaatgcaaa tactgtgaaa aagagttttc ccataatgtg gtgctggcca attaattgcg
     5221 gttggtaata aaagtctggc tccctataat gagccagact ttttaccgct gtaataaagg
     5281 agaaatcatg agcaaaacta tcgcgacgga aaatgcaccg gcagctatcg gtccttacgt
     5341 acagggcgtt gatctgggca atatgatcat cacctccggt caaatcccgg taaatccgaa
     5401 aacgggcgaa gtaccggcag acgtcgctgc acaggcacgt cagtcgctgg ataacgtaaa
     5461 agcgatcgtc gaagccgcag gcctgaaagt gggcgacatc gttaaaacca ccgtgtttgt
     5521 gaaagatctg aacgacttcg caaccgttaa tgctacctac gaagccttct tcaccgaaca
     5581 caatgccacc ttcccggcac gttcttgcgt tgaagttgcc cgtctgccga aagacgtgaa
     5641 gattgagatc gaagcgatcg ctgttcgtcg ctaatatcta ctgaaagata atccaggtat
     5701 tgcgcctggg ttatctgatt caaccacgga gggttgaaga tggctaaacc ccgtttcact
     5761 aacgaacaaa ttgctgaaat tttacagcag tcaaaagagg gggcgtctaa taaggagcta
     5821 tgtgaacact atcaatttag cgttagcacg ctgcggcgct ggcaagaaca gcacgctgac
     5881 ggcatcagaa gcgaattaaa aaaaacagaa tccaaagcac aaatcgtctt tctggttttc
     5941 tttgctatcg ctattctact gactcttata tttgacaaac ctacaggtgg atgggtaatt
     6001 ccgcctttat taatctattg tgtttattat atccgccagt atcgcaacat ctctggcaga
     6061 catattaaaa aagaagatat ctatctctct cgttcagtaa ataacagcta tagcgccctt
     6121 tataatttga gctggacgtt tatttgtttt
//
LOCUS       DAZGCS010000191         6029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGCS010000191 DAZGCS010000000
VERSION     DAZGCS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.194"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            534..719
                     /locus_tag="ACHK01_004753"
     CDS             534..719
                     /locus_tag="ACHK01_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099552.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(906..1295)
                     /locus_tag="ACHK01_004754"
     CDS             complement(906..1295)
                     /locus_tag="ACHK01_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099553.1"
                     /translation="MEFKDLLKEIQEIAEHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1307..1435)
                     /gene="ydfB"
                     /locus_tag="ACHK01_004755"
     CDS             complement(1307..1435)
                     /gene="ydfB"
                     /locus_tag="ACHK01_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO8099554.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1437..1592)
                     /locus_tag="ACHK01_004756"
     CDS             complement(1437..1592)
                     /locus_tag="ACHK01_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8099555.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1869..2156)
                     /locus_tag="ACHK01_004757"
     CDS             complement(1869..2156)
                     /locus_tag="ACHK01_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8099556.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2156..2347)
                     /locus_tag="ACHK01_004758"
     CDS             complement(2156..2347)
                     /locus_tag="ACHK01_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO8099557.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2375..2776)
                     /locus_tag="ACHK01_004759"
     CDS             complement(2375..2776)
                     /locus_tag="ACHK01_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099558.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            2885..3157
                     /locus_tag="ACHK01_004760"
     CDS             2885..3157
                     /locus_tag="ACHK01_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8099559.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3141..3566
                     /locus_tag="ACHK01_004761"
     CDS             3141..3566
                     /locus_tag="ACHK01_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099560.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(3773..4228)
                     /locus_tag="ACHK01_004762"
     CDS             complement(3773..4228)
                     /locus_tag="ACHK01_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099561.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4307..5422
                     /locus_tag="ACHK01_004763"
     CDS             4307..5422
                     /locus_tag="ACHK01_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8099562.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5429..>6029
                     /locus_tag="ACHK01_004764"
     CDS             5429..>6029
                     /locus_tag="ACHK01_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8099563.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVG"
BASE COUNT         1581 a         1328 c         1549 g         1571 t
ORIGIN      
        1 gtgcctccag gtgacgttaa ccagttaaca attaacgccg gatacagagc attctcgtta
       61 ctcagcaaat gaccgcttta ccgatttaac tgttccgcgt gcgctgagcc gcattcaccg
      121 catcacaaaa ttcactttta aaaaagggcg gcagagcagt cacggagtaa aactgatacc
      181 gccaaatgtc accagaatat tgataacaga gggcgttgca gcggggttgt cacttaagcg
      241 tatggtcaac ctgacaaccc ggtgtcctca acggggaaag aataaccccg ccatacttac
      301 cgccgcgcca tttcgcggat tgccacaacc ggaagcgcac ggtcgaatta aatttaacga
      361 cggctctaag ggagaaggac ttcgccgtgc actttcgtgt tatgccctga cttttcaggg
      421 atatatcctt ttagtaaact gtcagtgccg gattcttatc tgtgtccggc gcacgaccac
      481 acgtagcagc gtgttggtct ccatttttaa ttcagccctt aatggaggat aaaatgccaa
      541 acaaaccatg ccctgcctgt aacgctcttt ctggcttttt agagaagggc ggttattaca
      601 tttttaactg tccagaacat gttgaatttc atatatcaaa acttgacagt atcattacaa
      661 agccaaatca gtatcagtct gatttactga ataaagagtt aaacgccgcc cgagattaag
      721 ggcgtagtgg cccattaaaa atattgcaaa gaccaactat tgttgaactg gattaaacca
      781 gtaattctct attgctctta ttgttaccat cggatgattg ccgggggtta ttttaagttc
      841 gggaggaaaa ggctctccat cagcagccat cattgagtat gtgaaatccg ccgacgcagt
      901 ccctttcatg ataattccga atttttcaag taatgagagc agttccggta atcctgtgtc
      961 atctgaataa ctttcctcat agaaagaagc gattgccgca ggagatgaca gttctcgctc
     1021 tgcaattttt ttgtagtttt ccagaattct ttttttgaat acttttcctc gacatgaaaa
     1081 cccatcaccg aggcaatccg ctttgcagtt tcttcagtat tctggttgtt tgttaccgaa
     1141 acagagtaca atccggtaaa cgcatcgcgc acattacgag ccatattatc aatgtatttc
     1201 ttcgttgcag attctaattc aacttcgttc agacgttggt gaagtgcatg ttctgcaatt
     1261 tcctggattt cttttagtaa atctttaaat tccatcttta actccgttag tcgttagatt
     1321 ttgccgaact ggaaagcgcc tgtttaaact cactgaagct gagagcttcc tcgccttcgg
     1381 caaggccttc gaagtattct tcgtaagcct tttccatgat tgtgtcgaaa tccatatcac
     1441 tcacctgagt ttctttccag ccagcgacgg gcaccatttt cggttttaaa cgttttgctt
     1501 ttggtatacg tcatcgcggt gaatgtaccg tcctggttgg gaaacacgcc gtacaccaga
     1561 gattcgttgt tgccaagatc gatagtatcc atgttgacct catttcccct taacgccggg
     1621 tagcggaaca aaaacctgct gcatagttat taaagttgaa ccctgccgtc atgttcatac
     1681 gcctcgggct ggctacttac cccctgacca ctgcttggta actcgaagta ttgcccggcg
     1741 ttctgtgggg cggggtgggt ggttggtgta tgtaatctac aattaaaaac tgttttgtgt
     1801 caacagtttt taattgttgt tttgggcaaa aaaatccctc gaaagaggga gtatgaaaat
     1861 tgttcaactc agatagagaa gggaaattgt cgccgagaat gtgtcacgct tacaatctca
     1921 atgcttgaag cagctactct gtacaggatt atgtagttag ggtgggtcac gatctctctc
     1981 aatccagaaa cccgttcgct tggtggatat aagtacggat gctcagatag aggtaatacc
     2041 gatgtttcaa tgcgtatttt tagtctacgt gctgccggtg ggttctcctt ggcgatgtaa
     2101 gttatgatct ggcgcaaatc atcgcgagca gacggtagcc ataaaatggg taacattact
     2161 cgctcttgtt agttacagca atttgagcaa taagattttc catttcagcc attacctcgt
     2221 catgtggaat tgcggggcga gtgtctgcaa ggcttgacgt tactttagtg cgcaaccatt
     2281 cgttgtaact gttttcttgt tcggtagttt cgaattctga aactatcgga gaaagggctg
     2341 tacccatggc ataactcctc ttcttgtact gtggtcacgc ccggcggctt ttttgtgccg
     2401 ccagccacct agcaatggtt tcttccattg attttttctt gtctttgatt tcttgaagca
     2461 ttttttcttg gtcttcctca ggaaacgcac taaaagcttg gagcagttcg cgttggcgag
     2521 gaccaatttt catcgtgtca ggggtgaaaa tttgctcaca ctcttcagga ggcaataaaa
     2581 accaatgcaa tggatgccct gaaacctcaa ccagtttatc caaacttgag gctttaggtg
     2641 tagccttacc gctgacccat tgttgaacag tttgttgtgt cacaccaatt ctacgggcaa
     2701 gctcagcctg gctccatcca gtttcctgaa gaagcttgct gattctgtac atagatactt
     2761 ctagggcgct catcattatt caattttaca ggtaaatact gttaaaagca tcacaataaa
     2821 aaactgttga ttgcatacag ttttttattg taggctttgc ttatagtttt tagaggaggg
     2881 caaaatgcta gatagcactc gcgaaaaaat taggcagaaa tacactcagg ctgaaatagg
     2941 tcgttatatg ggggtcgctc aacagactgt ttggcaatgg tttagctttg gcgttccccc
     3001 aaagcaggta attccgttat gccaactaat gaagtgggaa gttaccccgc atgaaatccg
     3061 cccagatatt tatcctaacc caaccgacgg tttacctgtt ggatgtaagg ttaacacatc
     3121 aaatgcgccg gagttgattc atgaaaatca agcatgaaca catccgcatg gcgatgaatg
     3181 cctgggcgca tccggacggc gaaaaagtac cggctgcgaa aattaccaaa gcgtatttcg
     3241 agctgggaat gacgttcccg gaactgtatg acgacagcca tccggaagcc atggctcgca
     3301 atactcagaa aattttccgc tgggtggaga aagacactcc tgatgcggtt aaaaaaattc
     3361 aggcgttgtt accagcgatc gaaaaagcga tgccgcctcc gctggtggcc cgaatgcgca
     3421 gccacagttc cgcttatttt cgggagttgg tagagacgaa ggaacggctg gtgaaagata
     3481 ttgatgattt cgttgcatca gcgatcgttc tgttcgatca gatgaatcgt ggtggcccgg
     3541 caggaaacac tctggctgtg cattaattgg gtaataaata tgagtaatga caaaaaattg
     3601 acactgagcg tttacgaaaa cagtccgcac atctggcgtg gcggtttatc tgatgtggag
     3661 ctggcagagt ggttgataca taaagctaat gcgctgctct ggcgtttgtc agccagagaa
     3721 cagcgcaagg aaaccagaat aaagctggct gatgcagaag cgtgtgccgg gcttattgag
     3781 gattatacaa atcttggtat ttcttcagca gagagtgatc ccattcagcc tctgagcagg
     3841 gagtcaatcc agcacgctgg ttgtatggca catcttgtaa ctgctcgtca acatgaggtg
     3901 ggtattggat cacttccggt gggatattcg ctgattccag agctggttga agcaagaaaa
     3961 tcagttcaga aaaagagaga tgacgcactt caattattga gagagcacta tggcgcgata
     4021 ccagaatgcg aacagcatcg ataccctgaa ggttatgaat ggatgcagtc tctttttgaa
     4081 gttcgctaat caatatgtcg agacgaaggt atgtttcggc gcgcagccag gctctgtaat
     4141 ccgggagcat ttcggggctg ttacaccagc ggtttgttgc tgcaacattt aatacatgag
     4201 cctgataaag gcttttcaaa aaatacatgt cgaacctcct ctggttctgt cgattgggaa
     4261 ccacagatta tatccggagg aaggttcggc accagatgag gtagccatgc gtgattacgc
     4321 aaaagtttct ccgcgattct ggctgggaga aacggggaga gaacttagaa aggcgggtgc
     4381 agaagcgcaa gttgttgctt tttacctgat gacatcccct cacgcaaata tgctgggttt
     4441 gtattacctg ccagttttat accttgctca tgaaaccggg cttggtctgg aaggggcttc
     4501 aaaggggctt aaaagggctg ttgaagctgg tttttgtagc tatgaccatg atgcagagat
     4561 ggtctgggtc catgaaatgg cagcctggca ggttggggaa acgttgaagc ctggcgataa
     4621 ccgttgtgca ggtgtcagga atgagtatgc atcattacct gaaaacgctt ttctgtcagt
     4681 gttttacgac agatataaaa cggatttcca tctggatgtg aggcggaata atagccgaaa
     4741 ttcggtaagg ggcttcgaag gggcttttaa ggggcttcga agccaagaac aggaacagga
     4801 gcaggagaaa gaacaggaac aggacaaaaa cactatggtt catggcaaaa aaaacaccac
     4861 gaaccaggca ggggatgttc agaccgtcaa tcctggtcag ccagcaggca cgacaccgga
     4921 agccgattca gcgtatgcgc tgaaagccga ttcgggcgct gtgcagcagg tgatgaccgc
     4981 agggtcggag caatcacacc aactgcagca gcctgaagcc gattccgcca ttcagcggga
     5041 agccgatcgg gtagtcccgg aaagcaccgg gcagtctgtg ggacgagtgg attatccgga
     5101 tgtgttcgaa caggtctggc gggaataccc gttgcgtgct ggggcaaacc cgaagaaatc
     5161 cgctttcagt gcctggaagg ccagattgcg cgagggggtg ccaccagaga ccatgctgga
     5221 tggtgtgagg cgttacgcga gatacctggc ggcgaccggg aaagcgggaa cggaatttgt
     5281 tcagcgagcg acgacgtttt ttgggccgga ccggaatttt gaaaacccct ggttgctccc
     5341 ggtaagcggc acgaacaacc agcgttgtgt gaatcatatt tctgaaccgg ataccgaaat
     5401 tccgccggga ttcagggggt gatgtggcat gaaaaacatt gcggcagccg gggttcttga
     5461 acgtattcgc agacttgcac cacaggcgtc ggttccaccg taccggacgg tggaggagtg
     5521 gcgggaatgg caacttgctg aaggacgaaa acgcagcgag gagattaacc gccagaatca
     5581 ccagttgcgg gtggaaaaaa tcctgaatcg ttcgggcatc cagcctctgc acagcaaatg
     5641 ctcgtttgcg aattatcagg tgcagaacga cgggcaaaaa tacgcgctga gccaggccaa
     5701 atccatagct gacgaactga tgaccgggtg cacgaatttt gtgttcagcg gtaaaaccgg
     5761 caccgggaaa aatcaccttg cagcggcgat gggtaaccgg ctgatggcga aggggcgcag
     5821 cgtgattatc gtcaccgtgt ctgatgtcat gagcgtgttg catgacagct acgacaacgg
     5881 caaatccggg gaaaaatttt tacaggagct ttgcagtgtt gatttgctgg tcctggatga
     5941 aataggcgtt cagcgggaga cgaaaaacga gcaggtggta ttacaccaga taattgatcg
     6001 ccggacagca tcactgtgca gtgtcggga
//
LOCUS       DAZGCS010000192         5956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGCS010000192 DAZGCS010000000
VERSION     DAZGCS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.195"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..233)
                     /gene="yaiA"
                     /locus_tag="ACHK01_004765"
     CDS             complement(42..233)
                     /gene="yaiA"
                     /locus_tag="ACHK01_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO8099564.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(283..807)
                     /gene="aroL"
                     /locus_tag="ACHK01_004766"
     CDS             complement(283..807)
                     /gene="aroL"
                     /locus_tag="ACHK01_004766"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO8099565.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(990..1448)
                     /locus_tag="ACHK01_004767"
     CDS             complement(990..1448)
                     /locus_tag="ACHK01_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO8099566.1"
                     /translation="MTIWVDADACPSVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1568..2377
                     /gene="proC"
                     /locus_tag="ACHK01_004768"
     CDS             1568..2377
                     /gene="proC"
                     /locus_tag="ACHK01_004768"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO8099567.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2394..3509)
                     /gene="adrA"
                     /locus_tag="ACHK01_004769"
                     /gene_synonym="dgcC"
     CDS             complement(2394..3509)
                     /gene="adrA"
                     /locus_tag="ACHK01_004769"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO8099568.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3611..3931)
                     /gene="psiF"
                     /locus_tag="ACHK01_004770"
     CDS             complement(3611..3931)
                     /gene="psiF"
                     /locus_tag="ACHK01_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO8099569.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4050..5465)
                     /gene="phoA"
                     /locus_tag="ACHK01_004771"
     CDS             complement(4050..5465)
                     /gene="phoA"
                     /locus_tag="ACHK01_004771"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO8099570.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5566..5826)
                     /gene="iraP"
                     /locus_tag="ACHK01_004772"
     CDS             complement(5566..5826)
                     /gene="iraP"
                     /locus_tag="ACHK01_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO8099571.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1403 a         1597 c         1443 g         1513 t
ORIGIN      
        1 tgtagtggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt gtcagggtcc
       61 tcatagcgtt ttttcgcatc catcgcttcc tgagccgtcg gatgttcact gatcaacgag
      121 tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc tggctttcct
      181 ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg catcgttctt
      241 ccttatttca cgggatgaac gttaagtata ggcgctctaa aatcaacaat tgatcgtctg
      301 tgccagggcg ctacgaattt cagaaatcac ctggctgggt tcgtttgttg cgtcgatgat
      361 aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct gaacttcttc
      421 gctcagcggt tttcccgtta aggttggccg taaatcttct tccggtgcag cttgcagtcg
      481 gttaaccagg actgatactg gcgcacacaa ataaaccacg atcccgttat tttgcataaa
      541 gtgacgatta aattccgtca gaataatgcc gccgcctgta gcgataacgg tggatggcgc
      601 agttaccgct tccagcgccg ccgtttctct ggcgcgaaat cccgcccact cttccctttc
      661 gacgatctcc gcgaccgtca tattgagctg tgattgcaac cactgatcgg tatcgacaaa
      721 ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac agccccgagg
      781 cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg caatgcggcg
      841 aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt cgattgtaaa
      901 gaaaaaattc cacttaaagt gaaaatctca atacatccct tactatacca ataaatattc
      961 aagaatgaag tgtaaacaat aaattacatt tagccacgac tacgttgcac ttccagccac
     1021 cacttctcca gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct atctggtccg
     1081 ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt caggcgctca
     1141 cgaatggtcg ctggcgtgta acgttcgccg cgcggattaa gcgccgctgc gcctttctcg
     1201 atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc ttcacactgc
     1261 cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt acgaataaat
     1321 cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat ctgcatacgt
     1381 tccgccgcgc gatacaaaat ctctttaatt acactgggac acgcgtcggc atccacccaa
     1441 attgtcataa agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt ttgccttccg
     1501 gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc acggcaggag
     1561 tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa aagccattct
     1621 cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat acaccccctc
     1681 cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag aatcggcgca
     1741 agaagtggcg caaatcgccg acatcatttt tgccgccgtt aaacctggca tcatgattaa
     1801 agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt ctattgctgc
     1861 aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa ttattcgcgc
     1921 catgccgaac actcccgcgc tggttaatgc cgggatgacc tccgtaacgc caaacgcgct
     1981 ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg gcgaagcgga
     2041 agtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt cgccagccta
     2101 cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga tgccacgcgc
     2161 ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg tgctggaaac
     2221 gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca ccaccattga
     2281 agcggtacgc gtactggaag agaaaggctt ccgtgccgca gtgatcgaag cgatgacgaa
     2341 gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg acgtcaggcc
     2401 gccacttcgg tgcggttacg tccggctttc tttgctttgt aaagcgccaa atctgccgat
     2461 ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc cacactaatc
     2521 cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc acgcacccgt
     2581 aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc aaactcatcg
     2641 ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa tttgtaactg tcgggtaagc
     2701 gccacaatcg cttcatcgcc cacatcatga ccccaggtat cgttgatgct cttgaaatgg
     2761 tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt atcaaattca
     2821 ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc gtcgcgggta
     2881 ctcatgacct gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg gtagctgacc
     2941 cagccaaaca gcagaggata aacgacaata atgggaaggg agagccacca ttccagcggc
     3001 gcactattga acgacacggt aatgcccgta agctcgaggg tgacaaggca ggaaaccacc
     3061 atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa attcagacac
     3121 ataatcatca acatagcggt ggaaggcagc acgtttacgc ccattacgcc tacccacatt
     3181 cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg atcgacggcc
     3241 ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac caacaccagc
     3301 caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa gaacatgcca
     3361 gccaggccaa ccgcacgggg tagtctgacg cgacgggcga agcgcagccc ggaccgctga
     3421 tgttcgtttt gaggagttaa aggaggttcc tccccatgcg ccgccgcttt tttgaaaaag
     3481 ttttcatcat tcattatttt tgggaacatt cagaaacaat tttcccaaat tatagagacg
     3541 gggctaaagg cagaagtatg aaatttcggg tgagccactt tcgtagctca cccgttagca
     3601 ctatcaggta tcaggcggct ttcttgaggc aggcactcat aaacttatta cgatcatcac
     3661 ctttcagaga ttgttgcgtt gcttgattat tacattcgcg catcttttgc tgctgtggcg
     3721 tcaaactttt ttcaccaggc gcagacttgc tgttcttcag gcaatcactc atgtaggtct
     3781 tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg attacaggag gtcatacgct
     3841 gttgttgtgg ggttaaggtt ttctcggcag cgccgacggt ggttaaaaaa accagaccaa
     3901 aaagcaaggt aaccagtaat gttattttca tagcaccatc cctcttcatg ttttaaccat
     3961 gagcgtttgc gcccgtgatc tgccattaag tctggttgct aacagcaaaa aaaccacccg
     4021 gcagcgaaaa ttcactgccg ggcgcggttt tatttcagcc ccagagcggc tttcatggtg
     4081 tagaagagat cggtctggtc ggtcagtcca acaacattgg cggcatgcgg gccatacgcc
     4141 gcaatacgca actgactgcc ggtatgttct tgtgaatcct cttcagagtt cccgtaactc
     4201 atcaccatca ctgcgccatc tttagtattt agcgcctgag tgaggcccgg cgctttggta
     4261 tccggcgcaa caacctggct ggcgtgggcg tgatcagcgg tgactatgac cagcgtgtta
     4321 ccctcctttt tagcgaattc cagcgcccgt tgtacggctt catcgagatc gaccgtctcg
     4381 ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat cgattgacgc accttcaact
     4441 tgcaggaaaa atcctttctc atttttactc aacaattcaa tggctttgtc ggtcatctgt
     4501 gccagggttg gtacactgtc attacgttgc ggattaggcg tacaggtgac tgcgggctta
     4561 tcgatattgc cgtggtacgt tgctttcggc cctagccagc gcactggcat attgccgtca
     4621 gcaaacaggc caagcagggg tttttgctga ttcgcttccg tcaccgaatt cagtgaggca
     4681 gcatcgttca ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt ttttccctgc
     4741 cattcaccag cggttgccgt ttcagcaaag gtttttgcgc cgccgccaag cgtaacgtcg
     4801 gcacgagcgt taagcagctg ttcggtaatc gatccttttc cgcctttttc cagagcgtta
     4861 cccggacatt tttcactggt cgcgctcgga ccgtagcatt tgcgcgaagt cacatgtgcc
     4921 accagcgcag cgggcgtggc atcctgcaac tctgcggtag aaacgttacc ggtcgccaga
     4981 cctgcggctt ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat atcgacgccc
     5041 agcgcgccgt tataggtttt gacaccggtt gaccaggcgg ttgctgatgc agccgagtcg
     5101 gtgacgtagt ccggtttgcc agttttttta ttcagcgcat agtgagtgta ttgcccggta
     5161 agcggtaagg catcgatacc tttaaaaaag ccgcccgcac cttcggcata attacgtgcg
     5221 gcagtaattt ccgaatcccc catcccatcg ccaatcagca aaataatatt ttttgcaggt
     5281 ttatcgctaa gagaatcacg cagagcggcg gtatgatcac ccgttaagcg gcgagcaccg
     5341 ccgggtgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat ttctggagtc
     5401 cgggcttttg tcacaggggt aaacagtaac gttaagagtg ccagtgcaat agtgctttgt
     5461 ttcactttat tttctccatg tacaaataca ttaaaaaata aaaacaaagc gactataagt
     5521 ctcggccgtg acaactttat gacagctgtt gaaaagatta actttttact gacgaggatg
     5581 cttcaataac ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag gaatgctggc
     5641 atcgggcttt acctcgtaca gcgccccctc tacctgatca atcaaccgct gttggtcatt
     5701 ttgcgccata ttgcgaagca ttgcagtgac gataatctcc aaagcttcta cctgcgcaca
     5761 cagttctttc gactcttctt ctttttgggc aagcttaaat aacaactcag caatgagatt
     5821 tttcatgtct gtatttcctt atccaaagta tggagaagtt aacattatgt ttcacaactt
     5881 catagtgtct ggaagtatta tttttatgac aaaggaaata ctttgcgcaa atattttttg
     5941 tttgattacc agcgaa
//
LOCUS       DAZGCS010000193         5904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGCS010000193 DAZGCS010000000
VERSION     DAZGCS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.196"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..337
                     /locus_tag="ACHK01_004773"
     CDS             203..337
                     /locus_tag="ACHK01_004773"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099572.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            942..1763
                     /locus_tag="ACHK01_004774"
     CDS             942..1763
                     /locus_tag="ACHK01_004774"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO8099573.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1763..2869
                     /locus_tag="ACHK01_004775"
     CDS             1763..2869
                     /locus_tag="ACHK01_004775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8099574.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIECRFDINRTAQIIVSLASQAKGKCNR"
     gene            2959..4680
                     /gene="cptA"
                     /locus_tag="ACHK01_004776"
     CDS             2959..4680
                     /gene="cptA"
                     /locus_tag="ACHK01_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO8099575.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETPRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4754..5752
                     /gene="lpxM"
                     /locus_tag="ACHK01_004777"
                     /gene_synonym="msbB"
     CDS             4754..5752
                     /gene="lpxM"
                     /locus_tag="ACHK01_004777"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO8099576.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSAHQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
BASE COUNT         1524 a         1272 c         1481 g         1627 t
ORIGIN      
        1 agttcatgaa tgtttgcaaa aaaaaaaata aatgaacaac aattttatgt gtttaaaata
       61 aattaatttt acttctgtaa tggtgtcatg attagcagct ttttaaacta atatcgactt
      121 tcaataaata aggacaggta atggacaaca aaaatatctg aacccgggtg agattcataa
      181 gttgcacagt gctgtcttta aagtgccaca cccggaacgt aatcactgcc tgctccttat
      241 ggggtatctt catggtgtcc aagcatcaga gttattaggt ataaaactgt ctgatattga
      301 tttgcaggcc ggtaacctga atatccggcg gctctaaacg gtttgtcaac tcagcccctc
      361 ggctgaaaca ggaggtacag tccattcgtc gctggctgaa acttcgggat gaatgggtag
      421 aagagggata tgaatggtta tttaacctct atggcaggtc tgctacaggt attactgttg
      481 ttctgggggg ggggcactgg tgtggttaat atctacgatg caagagattc tcacgatggc
      541 ccggtccatc agtgcatgcg gcgcgatatt gtgcgagtat ttttcagggg gagtggtatc
      601 cggatgcaga aaattccgct actcgactgg ttttatgcaa taaatcagta tgataatctt
      661 ctgatttttt actgaggcgg atatctctcg ctgtgtttac tgaaattgcg gtattctgtt
      721 ttttctgaaa aacagaaaat ctgattaaaa agagcatatg cgttaaggga aatttaagtt
      781 ctggttgatt aactgataaa aatattaaaa aatccctgtt gtcggtaatc tccggaccat
      841 tccgggatta cacaataata tcaccatgtg tgatatctat ggatcctcac tgttgtttac
      901 gcgaggatat tttcagaatg cttaacagac gataattttt tatgttgaat gcaaggcatc
      961 ttcccgtact gatgtatcat catgtcagtc gttgtccggg gcttgtgaca ctctcacctg
     1021 aaacattttg tgaacagatg aagtggctcg cagataataa ctggcggacg gtaacatctg
     1081 ctgagcttga atatttttat caggggggaa cgcttccacg aaagagtgtc atgctcacat
     1141 tcgatgatgg ttatcttgat aactggctca gagcatggcc ggtcctgaag gaatataacc
     1201 ttcatgcaca tatttttctc attacggggc tgattggtaa gggaaacgta cgtagcaggc
     1261 aggagcagga atattctcac cgcgactgtg aacggttgat tgccgataac cgttcagatg
     1321 aggtcatgct tcgctggtct gaagtccggg aaatgcgtga ttccggactg gttgaatttc
     1381 atgtgcacac ccattcacat aaacgctggg acaggttgtc tgtatcacgt gctgaacagt
     1441 gccggctcat gaaagaagat attctggtgg gaaaacaatg tctgacggaa aagctggggt
     1501 tctgtagttc ccatctgtgc tggcctgaag gatattataa tcgggattat attaatctcg
     1561 ccggtaagct tggtttctct tatttatata caaccgaaag aagaatgaac tgtcctgaaa
     1621 atggttcatt acgcataggg cgtatcagca ccaaagagcg ggaacattcc ggctggctga
     1681 aacgacgcct tttttattat acaacgccac ttttttcttc agtactggct cttcataagg
     1741 ggccaagatt accagataat taatgatgaa aattttattc actgaatctt cgtcagatat
     1801 tgggggccag gagctgcagg cactggccca gatgactgct cttcaaaagc agggacattc
     1861 cgttttactt gcctgcaggg aaaaaagtaa aattgctccg gaagccagaa aaagagggca
     1921 tgatgtcact tttatccctt tcaggaacag ccttcacctt ccttccatcc tcagacttcg
     1981 acggattatc ggggaattta aacctgatct ggtcatctgc catagtggcc atgacagtaa
     2041 tatagcagga ctgagccgtc tgatatgctg tcaccgcttc agtattgtca gacaaaaaac
     2101 ctacatcacc cgtaagaccc gaacgttttc cctgaattat ctgtgtgatt tcattgttgt
     2161 gcctagctca gccatgatgg cgcatctgat ggcggaaggt gtcaggacac cggtaacggt
     2221 tattccaccg ggtttcgact ggcccgcatt gcataatgaa gccatgcgac cgttacccct
     2281 gcatatccac gcctgggctg catcagcaga caatgttccg cttatcgttc aggtggggat
     2341 gctacgtccc gaaaaaggac atgaatttat gctgcgtgta ttgtatcagc tgaagatgga
     2401 agggaaatca tttcgctggc tcgtggtggg cgccgggcgt gaagaatacg aagcacgtct
     2461 gcgtcagcag acagaacacc tgggaatgag cggagatgtg ttaatggcag gtgccctttt
     2521 cccagcgtta ccggtctacc ggattgccag tgtggtggtc atgccttcgg aaaatgaagc
     2581 gtttggtatg gtactggctg aagcgtcagt ctctggcgtg cccgttatcg ccagtgagac
     2641 gggcgggatc cctgatgtca ttcagaagaa tgtgacaggg acgctattac ccgtggggga
     2701 tgtgtctgca tggaccggcg cactgcgcga ttttttatcc cgaccggaac gcttccggat
     2761 gatggcagcc agcgccagag aagatattga gtgccggttt gatattaaca gaacggcaca
     2821 gatcattgtg tctctggcta gtcaggctaa ggggaaatgt aacagataac ggcggatcct
     2881 cttttcagcc cgaattttgt tcagcggttt tccggtaacg gagtaattta tcacattgtt
     2941 taaatacggg gacggtcaat gcatttaaat accggacaga acaggcctac tttcagctgg
     3001 agtgcgctgg gatgggcaat tttttatttt ggcttttttt ccactctcct gcaggtcatc
     3061 atcttcagca gtgggtacag cggaacgaat ggaatacggg actcactgtt attcagttgt
     3121 ctgtggttga tcccggtgtt tctctatcct gatcggataa aaataattgc agctgttgtc
     3181 ggtttcattc tctggggcac gtcgctggca gcactttgtt attattttct ctatggtcat
     3241 gaattctctc aaagtgttct tttcgttatg tttgaaacga atgccagaga ggctggtgaa
     3301 tatttcagcc agtatttcag ccttaaactg ttgcttatct cgctggtata tactgcggtg
     3361 tccgtttttc tgtggacacg tctgcgtcct gtatatattc ctttgccatg gcggagaatt
     3421 gtctctttcc tgctgcttta tgctctgctt ctgcatccgg ttgttctgaa atcgttaatc
     3481 agacaggagc cgctgaatga tactctaggc aaactggcat cccgaatgga gcctgctgcc
     3541 ccctggcagt ttgtatccag ttattatcag taccatcagc aactgaatgc actgacaacc
     3601 ttcctgaatg aaaatagcgc actgccacca ctgggtaatc tcagggatga atcaggggag
     3661 agaccacgca cactggtcct ggtgattggt gagtcgacac agcgcgaacg catgagctta
     3721 tacgggtatc tacgtgaaac gacgccggag ctggatgcac tgcgtaaaac cgatccgggt
     3781 cttactgtgt ttaataatgt ggtggcatcg cgtccgtaca ccattgaagc attgcaacag
     3841 gcccttactt tcgccaacga aaagaatcct gatctgtatc tgacgcagcc gtcgctgatg
     3901 aacatgatga agcaggcagg ctataaaaca ttctggatta ccaaccagca gacaatcaca
     3961 gcccgtaaca ccatgctcac tgtattttcg cgccagacgg acaggcagta ctacatgaat
     4021 cagcagcgaa cacaaagtgc gcgtgaatat gacactaacg tgctgaagcc gttccgggaa
     4081 gtgctgaatg accctgcacc aaagaaactg atcatcgtgc atctgctggg tacgcacatt
     4141 aagtataaat accgttatcc ggaaggtcag gggcggtttg atggcattac agggcatatt
     4201 cccactggat taaatgcgaa agagctggaa gtgtataacg attacgataa tgccaatctg
     4261 tttaacgatc atgtggtggc cagtctgata aaggacttca gagcaactgc gccggacggc
     4321 tttctgcttt atttttcaga tcatggtgaa gaagtatatg atactccgcc atataaaacg
     4381 cagggacgga atgaagataa ccccacacgt cccatgtaca ctgttccgtt cctgctgtgg
     4441 acctcggaaa agtggcatgc tgcacatccg cgagattttt cgcagtatgt tgaccgcaaa
     4501 tacagtctgg ctgaactgat ccacacctgg tcagatttgg cgggactgac atacgatggt
     4561 tacgatccga cccgttctct ggtgaatccg caattcaggg aaaccccccg ctggattgga
     4621 aatccgtata aaaagaatgg gctcactgat ttcgacactc ttccgtatgg tgagccatag
     4681 tattttctta tcttcggttc cgttctgaag gcaggaggat gtgttttcct ctgctgtccg
     4741 gtggcctcca tggatggtac caccttctgc tgtactctgt tatcataatg agataagcag
     4801 gcagataccg gtaaatatga aaaatatcag aactgagttt attccccgct ttaaccttac
     4861 attatgcttc ccccgttact ggatgacgtg gacaggtatc ggtattatct gtgtgtttgc
     4921 aatggtcccc cccgcactga gagatccatt gttgggaaaa ttgggcatgc tggttgggcg
     4981 tctggggaaa agcgcacatc aacgggcctt gattaattta tcgctttgtt ttccggagta
     5041 cagtgataaa gaaaaggaga atatcgttga tgccatgttt gcaacagcct ccatggccgt
     5101 tgtgcttatg gctgaattag cactgtccgg gcctgataaa atttcgcatc gtatacgctg
     5161 gaatggtctg gagattgtgg agaagatggc acagaacaat gaaaaggtca tttttcttgt
     5221 gccacatgcc tggggagtgg atattcccgc aatgctgatg gctgcgtcgg gcagaaaaat
     5281 ggcagcgatg tttcataacc agagaaatcc ggtggtggat tatgtctgga acagtgttcg
     5341 tcgtcgtttc ggaggaaaac tgcacgcacg taatgacggt attgcctcct ttgtccgctc
     5401 agtccggcag ggatactggg gatattatct tcctgatcag gatcatggcc cggaattcag
     5461 tgagtttgca gatttttttg cgacgtataa agcaacttta ccggttatcg gacggctcag
     5521 caggatcagt ggtgcacgga taataccgct tttccctgtt tatgacggaa agacccatca
     5581 tctgactatt catgtcagcc cgccacttgc tatacgacag aagagtgatg ctcatattgc
     5641 gcgacagata aacgaagtgg ttgaaaattt cgttcgtcca catcctgagc agtatacgtg
     5701 gatcctcaag ttgctgaaaa cgcgtaaaga aggggaagag gatccttact gaggcaggtt
     5761 tttcaggata atgagcttac ccagcaatag tggacacacg ctaagtgagt aaactctcag
     5821 tcagaggtgg ctcatatgac aaaagtaagc gtacagcctg aaccgtctgg tcagaatctg
     5881 acgaattaga caaagtggtg tcca
//
LOCUS       DAZGCS010000194         5902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGCS010000194 DAZGCS010000000
VERSION     DAZGCS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.197"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="ACHK01_004778"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="ACHK01_004778"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO8099577.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="ACHK01_004779"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="ACHK01_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO8099578.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3130..3273)
                     /locus_tag="ACHK01_004780"
     CDS             complement(3130..3273)
                     /locus_tag="ACHK01_004780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099579.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRPLTIE
                     TRD"
     gene            3456..3779
                     /gene="yiaW"
                     /locus_tag="ACHK01_004781"
     CDS             3456..3779
                     /gene="yiaW"
                     /locus_tag="ACHK01_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8099580.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3785..4921
                     /gene="yiaV"
                     /locus_tag="ACHK01_004782"
     CDS             3785..4921
                     /gene="yiaV"
                     /locus_tag="ACHK01_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8099581.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4918..5892)
                     /gene="yiaU"
                     /locus_tag="ACHK01_004783"
     CDS             complement(4918..5892)
                     /gene="yiaU"
                     /locus_tag="ACHK01_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8099582.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1456 a         1416 c         1542 g         1488 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt
      181 taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg
      541 cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttttgct gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct
     1141 gtggttttac taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag
     1201 cgatgtaatc atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaaccctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc
     1441 ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca
     1501 gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgc gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct
     1681 gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga
     1741 tgtaccgctg gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg
     1801 tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt
     1861 ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaatcg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 cggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat
     2161 tccggtgacg ctggagctgg gcggcaaatc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggtgaagtt tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgcagc ggtaacccac tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg cagacgtgct caccggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcaggcg tctggagtcg
     2701 taacggtaat ctggcctata agatggggcg cggcattcag gccgggcgcg tgtggaccaa
     2761 ctgctatcac gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga atcaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat
     3241 ctgtactgtg gatgttttac tggagttatt cattagtttg gccaatagtt ccattatttc
     3301 aatatgataa gtctcgacct gttgaaaagc caggtagcac acttcagaaa gtatataaaa
     3361 aacaatatgt cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc
     3421 gttaattgtt cttttttata ttctctgagg gaaatatgtt cctggactat tttgcactgg
     3481 gagtgcttat ttttgtattt ctggtgatct tctatgggat tattatttta catgatattc
     3541 cctacctgat tgctaaaaaa cgtaatcatc ctcatgccga cgccattcat gttgctggtt
     3601 gggtgagtct ttttacgtta catgttatct ggccattttt gtggatttgg gccacgcttt
     3661 accgcccgga gcggggatgg ggaatgcaaa accatgactc ctccgttgtg cagctacaac
     3721 agcgtattgc cgggctggag aaacagctcg ccgacatcaa atcctcttct gccgagtaat
     3781 acttatggat ttactgatta ttttgaccta tgtggctttc gcatgggcaa tgtttaagat
     3841 cttcaaaatt cctgtaaata aatggaccat tcccacagcg gccctgggtg gcatatttat
     3901 tgtcagtggt ttaattctgt taatgaacta taaccatccg tataccttca aagcgcaaaa
     3961 agcggttatt tctattcctg ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga
     4021 taagaaaaat acgctgatta aaaaaggtga ggtgctattt cgactggacc cgacgcgtta
     4081 tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc gcagaacata aacagcgggc
     4141 gttgggcgca gagttagatg agatggcggc gaatactcag caggcaaagg ccacgcggga
     4201 taaattcgct aaagagtatc agcgttacgc acgcggcagc caggcgaaag taaacccgtt
     4261 ttcagaacgc gatattgatg tggcgcggca aaattatctg gcgcaggaag cctccgtaaa
     4321 gtcatcggcg gcggaacaaa aacagatcca gagccagctg gatagcctgg tgttgggtga
     4381 acattctcaa atcgccagcc tgaaagcgca gctcgcggaa gcaaaatata accttgagca
     4441 gacgatagtg cgtgcgccga gcgatggtta tgtgacccag gtgctgattc gtccgggtac
     4501 ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt atacccgatc agaaacgaca
     4561 aattgtggcg cagttccgtc agaactcctt gctgcgactg gcccctggcg atgatgcgga
     4621 agtggtattt aacgctctgc caggtaaggt attcagcggc aagctggcag ccattagtcc
     4681 agctgttccc ggcggagctt atcagtcgac cggcacctta cagacgttaa acacagcgcc
     4741 gggttcagat ggcgttatcg cgaccattga actggatgag cacactgatt tgagcgcttt
     4801 accagacggt atttacgccc aggtggcggt ctactctgat catttcagcc atgtctcggt
     4861 gatgcgcaaa gtgctgttac gcatgaccag ttgggtgcat tacctttatc tcgatcatta
     4921 atcttcgctt tccgccgctt gttcaggccg cagttgctgc gactgagcaa gctggtggcg
     4981 aatttcacgc agcatttcat ccagcacata ctggacccgc cagggctggt attcccgttt
     5041 acggaagata gccaccagat ccagccacca ttcatacggg tgctgaaagc agggcaccag
     5101 cgtgccgtct tgcaggtcgc tctgcacact tttgtctggc gcaaacacaa tccccaggtg
     5161 atttcgtgcc agctccagcg ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg
     5221 ataatcccgc acctctttac tcccggcgac gttgaagcgc cagatattcg cgtcgtcaat
     5281 catcatcgag tcgatcaaaa tacaggagtg atgttcgagt tcgtcaggac ggctaatagg
     5341 atgtttctca agatagcgct ggctggcata agcggttacg gcatattggg taataaaact
     5401 ggcaaccagc gattcatctt ttggtggagc gtagctgatt aaaacatcac aatcatcagg
     5461 aaattcgacg ccttcataaa aggcattacg ctcaagattg caggttttta gcgataaggt
     5521 gatatcaccg atatctttaa ttttatcgat tacatgtttg gataaatagg taattatgcc
     5581 ggttggggcg tagatagtga cccgaccacg tttctcatgc ttataatctg caataaaatt
     5641 attaagttgc tcgtttctat ccagcatgtc gttgatgtac ggtaacagcg cggtgccaaa
     5701 aggtgtgagc atcagctgcc gggtggttcg gtcaaagact tttaaaccca cttttgattc
     5761 aaaatcagca agatatttgc tgacgttggc ctgtgcgatg ccaagtacag tcgcggcatg
     5821 gctgatattt tcactggcag cgattaccga gataattttt aactcccggt acttaagttg
     5881 taattttgtc atcgcaacag cc
//
LOCUS       DAZGCS010000195         5845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGCS010000195 DAZGCS010000000
VERSION     DAZGCS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.198"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ACHK01_004784"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ACHK01_004784"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO8099583.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHK01_004785"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHK01_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO8099584.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHK01_004786"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHK01_004786"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO8099585.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..4421
                     /gene="rfaL"
                     /locus_tag="ACHK01_004787"
     CDS             3213..4421
                     /gene="rfaL"
                     /locus_tag="ACHK01_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO8099586.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4457..5599)
                     /locus_tag="ACHK01_004788"
     CDS             complement(4457..5599)
                     /locus_tag="ACHK01_004788"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO8099587.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLEKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5608..>5845)
                     /locus_tag="ACHK01_004789"
     CDS             complement(5608..>5845)
                     /locus_tag="ACHK01_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO8099588.1"
                     /translation="DDTVFIHYTGITKPWHDWANYASADYFRNIYNISPWRNIPYKKA
                     VKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1620 a         1272 c         1300 g         1653 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aatctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatat agcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctcggcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttaaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcactgg tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct
     3301 attttttggg tgggattaca aggtataaac atcttattgt tattcttatg acaataacga
     3361 caatcgtcta tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt
     3421 ttggtagtgt tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg
     3481 caggtgctag catgaaggaa attttcaaag ctattattga gaatacttta ctatgcacaa
     3541 tagcaatacc gatcatattg agagacgaga aaagagaaga tgtcgaaaaa atcgttttct
     3601 tctcatttat tagtgcgttg ggcttacgct gtttttctga attgattacc tattataagg
     3661 actatcaaca agggataatg ccattcgcag attatagaca ccgtagcatt tctgactcga
     3721 tggtcttttt attccctgca ttgttaaatc tctggcttat caaatcagca aaataccgca
     3781 tttcttttgt ggttctaagc gttattttta tttttctgat attaggaact ttatccagag
     3841 gggcctggct ttccgtgtta gtcattggat taatatggat tctgatgttt aaacaatgga
     3901 agttactatt agtaggagta atggttgcca tcattgcatt gtcggttatt ttcacacata
     3961 aggagatgac cgcaaagcta acgtataaac ttcaacaaac taatagttct tatcgctatg
     4021 caaatggtac tcaaggcagc gcactcgatc taatattaga aaatcctgtt attggttatg
     4081 gttacggtaa cgttgcatat aaagatgtct ataataaacg tgtcattgat tatccagaat
     4141 ggacctttag acaatcaata gggccacata attttgcgct attcatctgg tttggcactg
     4201 gtttattagg gctggtaagt cttatgatgc tatactgtgc aatattgaaa gagtgtataa
     4261 aaaatggcgt caagaataaa tatcgctcac catataatgc atattatata atcttactat
     4321 cttttatagg ttattttgtt atccgtggaa acgtagaaca aattgaacca aatttattag
     4381 gcgtttacgc cggcttatta ttagcgatga aaaacaagta aaaaataaaa aaggctgcat
     4441 aatgcggcct ttttatttat tcaaaccaat tatgaataac ctcttcgaat ctttgagtta
     4501 cgccatccca actgtagtgt tcaaacacaa agtcctgacc ttgctttgca actgctgtta
     4561 attcaggatt cgctaacgtt tttaaaatat cgctgcttat ggagtctgca gtcataggtt
     4621 cttttaaatg gaatcccgta gtattttctt tgacaaattc cgtcatcccc ccacgcgtac
     4681 tcactaaaac aggcttacca gcacccatag cttcaattgc caccatgcaa aatggttcct
     4741 ggaactggga aggaatgacg actaaatctg ccagtgggta ataacaatgc atcttttcag
     4801 gcggtatgct tcctaacata atgcaacgat ccttcaaacg ttttgcaatt tccctgacat
     4861 tgcgttgata tgcacctttg tcacttttac tcatttctgt ataatcacca atcacaacta
     4921 attttaaatt tttgtgagca gcggccaatt tttcgaatga ttgcatcaac agcaatatgc
     4981 ctttgtcagg gacgatacgc ccagcataaa aaataatttt ctcttcaggg gagatattaa
     5041 gttcgcttct agtgatcggc tgaaaattag attgataggt ttctaaatca attccatttg
     5101 gtacaatctc aatatctgca tttgctaaat aagattggta atacttttcc aagtataaac
     5161 taggcactat cattttaaca ttttgctcaa gcaattttgg ttcaaatgca ttatgcatat
     5221 gtataaccac tcttgcctga ggagctcgct tacgaatttg agtgtatagc ttcatgctat
     5281 tatgaactac aataacactt tctttcgtta taggaaaatc atgtgcaata ttcaatattc
     5341 tctgtgaata tggaagagga tcaagtcttg tccatttttg aaataaccgc ttatatatgc
     5401 gactgaatcc aaccctatga atactacaat tatcattaac aaaggtgtaa tttgagtatc
     5461 ctggattttt gatacaaaca atacgatttg gaaaattggt acgctgagca acctgataca
     5521 tccacgtttc aacagcagcc gctccgcgag gaggaattga aaaaatagga gtaaccgtaa
     5581 atattatttt atcaaccatc tcgtatatta acctttcata acattatatt taattgctgt
     5641 aaaaacacca tcgagaaatt ttttctggta aagcaagtgt ttatattttt ctttgtactc
     5701 atgtttttta acagcttttt tataaggtat atttctccat ggtgatatat tataaatatt
     5761 acgaaaataa tctgcagagg cgtagttcgc ccaatcatgc catggcttag ttataccagt
     5821 ataatgtatg aagacagtgt catca
//
LOCUS       DAZGCS010000196         5843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGCS010000196 DAZGCS010000000
VERSION     DAZGCS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.199"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..3205
                     /gene="agn43"
                     /locus_tag="ACHK01_004790"
                     /gene_synonym="flu"
                     /pseudo
     CDS             99..3205
                     /gene="agn43"
                     /locus_tag="ACHK01_004790"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
     gene            3326..5842
                     /locus_tag="ACHK01_004791"
     CDS             3326..5842
                     /locus_tag="ACHK01_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099589.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
BASE COUNT         1393 a         1535 c         1691 g         1224 t
ORIGIN      
        1 gggaaaaacc actttatctg accatgctgt tttgtacctg ccggtaccca catctgtggg
       61 taccggcttt tttattcacc ctctgtaagg aaaagctgat gaaacgacat ctgaacacca
      121 gctacaggct ggtatggaat cacattacgg gcaccctggt ggtggcctcc gaactggccc
      181 gctcacgggg aaaacgcgcc ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag
      241 tcccggcact ggctgctgac acggttgtac aggcgggaga aaccgtgaac ggcggaacac
      301 tggcaaatca tgacaaccag attgtcttcg gtacgaccaa cggaatgacc atcagtaccg
      361 ggctggagta tgggccagat aacgaggcca ataccggcgg gcaatgggta caggatggcg
      421 gaacagccaa caaaacgact gtcaccagtg gtggtcttca gagagtgaac cccggtggaa
      481 gtgtctcaga cacggttatc agtgccggag gcggacagag ccttcaggga cgggcagtga
      541 acaccacgct gaatggtggc gaacagtgga tgcatgaggg ggcgatagcc acaggaaccg
      601 tcattaatga taagggctgg caggtcgtca agcccggtac agtggcaacg gataccgttg
      661 ttaataccgg ggcggaaggg ggaccggatg cagaaaacgg tgataccggg cagtttgttc
      721 gcggggatgc cgtacgcaca accatcaata aaaacggtcg ccagattgtg agaactgaag
      781 gaacgacaaa taccactgtg gtttatgccg gcggcgacca gactgtacat ggtcacgcac
      841 tggataccac gctgaatggg ggataccagt atgtgcacaa cggcggtaca gcgtctgaca
      901 ctgttgtgaa cagtgacggc tggcagattg tcaaaaacgg gggtgtggcc gggaatacca
      961 ccgttaatca gaagggcaga ctgcaggtgg acgccggtgg tacagccacg aatgtcaccc
     1021 tgaagcaggg cggcgcactg gttaccagta cggctgcaac cgttaccggc ataaaccgcc
     1081 ttggagcatt ctcggttgtg gagggtaaag ctgataatgt cgtactggaa aatggcggac
     1141 gcctggatgt gctgaccgga cacacagcca ctaatacccg cgtggatgat ggcggaacgc
     1201 tggatgtccg caacggtggt accgccacca ccgtatccat gggaaatggc ggtgtactgc
     1261 tggccgattc cggtgccgct gtcagtggta cccggagcga cggaaaggca ttcagtatcg
     1321 gaggcggtca ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg
     1381 gtgatatggc cacggatacc acggtaaatg gcggactgtt caccgccagg ggcggcacac
     1441 tggcgggcac caccacgctg aataacggcg ccatacttac cctttccggg aagacggtga
     1501 acaacgatac cctgaccatc cgtgaaggcg atgcactcct gcagggaggc tctctcaccg
     1561 gtaacggcag cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca
     1621 cccagaaagc cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca
     1681 cggatgtcat tgctcagcgc ggtacagccc tgaagctgac cggcagcact gtgctgaacg
     1741 gtgccattga ccccacgaat gtcactctcg cctctggtgc cacctggaat attcccgata
     1801 acgccacggt gcagtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct
     1861 ccacccgcac agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga
     1921 atggcaccat cagcctgcgt gtacgcccgg atatggcaca gaacaatgct gacagactgg
     1981 tcattgacgg tggcagggca accggaaaaa ccatcctgaa cctggtgaac gccggcaaca
     2041 gtgcgtcggg gctggcgacc agcggtaagg gtattcaggt ggtggaagcc attaacggtg
     2101 ccaccacgga ggaaggggcc tttgtccagg ggaataagct gcaggccggt gcctttaact
     2161 actccctcaa ccgggacagt gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg
     2221 cagaagtccc cctgtatgcc tccatgctga cacaggcaat ggactatgac cggattctgg
     2281 caggctcccg cagccatcag accggtgtaa acggtgaaaa taacagcgtc cgtctcagca
     2341 ttcagggcgg tcatctcggt cacgataaca acggcggtat tgcccgtgga gccacgccgg
     2401 aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggttg
     2461 ccggtatgtc tgtgaccgcg ggggtatatg gtgctgctgg ccattcttcc gttgatgtta
     2521 aggatgatga cggctcccgt gccggcacgg tccgggatga tgccggcagc ctgggcggat
     2581 acctgaatct gatacacaac gcctccggcc tgtgggctga cattgtggcc cagggaaccc
     2641 gccacagcat gaaagcctca tcggacaata acgacttccg cgcccggggc tggggctggc
     2701 tgggttcgct ggaaaccggt ctgcccttca gtatcactga caatctgatg ctggagccgc
     2761 aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac gccggttatg
     2821 tgaagttcgg gcatggcagt gcacaacatg tgcgtgccgg cttccgtctg ggcagccaca
     2881 acgatatgaa ctttggtaaa ggcacctcat cccgtgacac cctgcgcggc agtgccaaac
     2941 acagtgtgcg tgaactgccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca
     3001 gctcccgggg ggacatgagc atgggtacag ccgcagccgg cagtaacatg acgttctcac
     3061 cgtcacagaa tggtacgtca ctggactgga agcccgtgtc cgggaaaata tcaccctggg
     3121 cgttcaggca ggttatgtcc acagcgtcag cggcagcagc gctgaaggct ataacggtca
     3181 ggccacgctg aatgtgacct tctgacagaa ccatcgcctc tctgtggtcc cggtcatcat
     3241 gatcgggacc cggaccggcg caacggatat tcaacgccac attcgctggc attaacaata
     3301 acatgatatt catcacggag tgactatgtt acagatagtc ggcgcgctga tcctgctgat
     3361 cgcaggattt gccattcttc gccttttgtt cagagcatta atcagcacgg cttctgcgct
     3421 ggcagggctc atattgctgt gtctgttcgg cccggcctta ctggctggct atatcaccga
     3481 acgcataacc cggttgttcc atattcgctg gctggcaggc gtatttctga cgattgccgg
     3541 aatgatcatc agcttcatgt ggggacttga tggtaaacat atcgcgctgg aggctcacac
     3601 ctttgactct gtgaaattta ttctgaccac cgctctcgcc ggtggtctgc tggctgttcc
     3661 cctgcagatc aaaaacattc agcagaacgg gatcacacca gaagatatca gcaaggaaat
     3721 taacgggtat tactgctgtt tttatactgc ctttttcctt atggcgtgtt ctgcatgcgc
     3781 accattgatc gcgttacagt acgatatttc accgtcactg atgtggtggg gcgggttgtt
     3841 gtactggctg gctgcattag tgacgctgct atgggcggcc agccaaatcc aggcgctgaa
     3901 aaaactgacc tgtgccatca gccagacact ggaagaacaa ccggtgctca acagtaaatc
     3961 gtggctgacc agtttgcaaa acgattacag ccttcctgac tcactgacgg agcgcatctg
     4021 gctgacgctc atttctcaac ggatttcccg gggagagctg agggaatttg aactggcaga
     4081 cggaaactgg ttactgaaca atgcctggta tgaaagaaac atggcagggt ttaacgaaca
     4141 gttgaaagag aacctgtcat tcacacctga tgaactgaaa acgctcttcc ggaaccgcct
     4201 gaatttatca ccggaagcga atgacgattt tctcgatcgt tgcctggacg gcggtgactg
     4261 gtatcccttt tcagaaggtc gccgttttgt atcattccat cacgtggatg agcttcgtat
     4321 ctgtgcctcc tgcgggctga cagaagtaca tcatgccccg gaaaatcata agccggatcc
     4381 ggaatggtac tgctcctctc tttgtcgcga aacagaaaca ctgtgtcagg aaatttatga
     4441 acgcccttac aacagcttta tttccgatgc aacggcgaat ggtctgattc tcatgaaact
     4501 gccggaaacc tggagtacaa atgagaaaat gtttgcttcc ggagggcagg gacatgggtt
     4561 tgccgctgaa cggggaaacc atattgtcga cagagtccgt ctgaaaaacg cacggatcct
     4621 cggtgataat aatgccagaa atggagcaga cagactggtc agcggaacag aaatccagac
     4681 gaaatattgt tcaactgcag cccgtagcgt cggtgcggca ttcgacggac aaaacggaca
     4741 gtatcgttac atgggaaata atggccccat gcaactggaa gtcccccgtg atcagtatgc
     4801 cggcgctgtt gaaaccatga ggaataagat ccgcgaaggt aaagtacccg gtgtaaccga
     4861 tcccgcagaa gcatcccggc tgattcgtcg gggacatctg acttatactc aggcccgtaa
     4921 tatcacccgg ttcgggacca tcgaatcggt cacttatgat attgccgagg gttcggttgt
     4981 cagtctggcg gccggaggga tcagttttgc cctgacggca tcggtcttct ggctcagcac
     5041 cggcgatcgc gatgctgccc tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg
     5101 cacactggct gtctacgtca caacccagca actccaccgg ctcagtgttg ttcagggtat
     5161 gctgaagcac attgattttt cgactgccag cccgactgtc cgactggctc ttcagaaggg
     5221 gaccggtgca ggaaatatca gtgccctgaa caaagtgatg aagggtacgc tggtgacatc
     5281 tctggcactg gtagctgtca caaccggccc tgacatgatc aaaatgttgc ggggacggat
     5341 ctccggtacg cagttcatca ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc
     5401 tgtcgggtca gtggcgggcg gaatactgtt cagtccactg ggaccatttg gtgcactgac
     5461 agggcgtgtg gttggcggtg ttctgggggg aatgattgcc tccgctgtat caggaaaaat
     5521 tgccggagcg ctggttgaag aagatcgcgt caaaattctg gcaatgattc aggagcaggt
     5581 gacatggctt gccggcagtt tcctgctgac cggacatgag attgaaaatc tgaacgcgaa
     5641 tctggcccgt gttatcgatc agaatgctct ggagatcatt ttcgccgccg gtatacaaca
     5701 acgggccgcg accaatatgt taatcaaacc actggtggtc agtatcatca ggcaacgccc
     5761 cgtcatggaa tatgatgcat cccatctcgg caatatggtt aaccgactgg aagaagcatt
     5821 acccccggag ttaccggcat aac
//
LOCUS       DAZGCS010000197         5828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGCS010000197 DAZGCS010000000
VERSION     DAZGCS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.200"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..667
                     /locus_tag="ACHK01_004792"
     CDS             <1..667
                     /locus_tag="ACHK01_004792"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8099590.1"
                     /translation="TATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVIL
                     LQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGI
                     DVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAGD
                     AFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQTI
                     FTH"
     gene            681..1439
                     /locus_tag="ACHK01_004793"
     CDS             681..1439
                     /locus_tag="ACHK01_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO8099591.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            1496..2461
                     /locus_tag="ACHK01_004794"
     CDS             1496..2461
                     /locus_tag="ACHK01_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8099592.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            2464..3498
                     /locus_tag="ACHK01_004795"
     CDS             2464..3498
                     /locus_tag="ACHK01_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO8099593.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3538..3830)
                     /locus_tag="ACHK01_004796"
                     /pseudo
     CDS             complement(3538..3830)
                     /locus_tag="ACHK01_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(3910..5529)
                     /locus_tag="ACHK01_004797"
                     /pseudo
     CDS             complement(3910..5529)
                     /locus_tag="ACHK01_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1520 a         1402 c         1350 g         1556 t
ORIGIN      
        1 aactgccacg atccttgtta atgaaggtac tggcgataat gttattgcga tctatcccgg
       61 tgccaacatg acaatgtcgt ccgatgaaat aactattcag aaagaagcga ttattaattc
      121 tgatgtcata cttctgcagc tcgaaacaaa ttataccgct ctgcaacaag cgatcacatt
      181 ggcgcaaaaa aacagtatcc cggtcattat taacccggca ccctataatg atatcgtcaa
      241 cgaacttatt caggatgttg attatatcac ccccaatgaa acagaagctg gtttactgtc
      301 cggcatagac gtacacgatc ttgaatcagc taaacgagct gcagaagcta tccacaataa
      361 aggggtaaaa aacaccgtta tcacgctcgg tagcaagggc tcgctcgcat ttgatggcaa
      421 aaaattcatt cattcgccag cgtttccagc ggtcgttaaa aatacggcag gagccgggga
      481 tgcttttaac ggcgctctgg catcaggact tgcgaaagga aaatcactgg aatcagcact
      541 gtgttatgcc agcgcctttg catccctagc ggtggagaca agcaatgctt ccgacatgcc
      601 tgaacatgaa tctgtgatac atcgaattca gagtatacat tatcagcaaa ctatttttac
      661 gcattaagaa gggacagaga atgaataaca ccaaaaaatc actaaaggtg ctctttattg
      721 gagaatcctg gcatattcat atgattcatt caaaaggata tgacagtttt acctcaagta
      781 aatatgaaga aggtgcgacc tggttactcc aatgtctgaa aaatagtcag gtagatgtca
      841 cctatatgcc ggcacatacg gtacaaattg ccttccctga agatgtggca cagttagaac
      901 aatatgatgc catcgttatt agtgatattg gtagtaatac tttcctgcta caaaatgata
      961 ctttttatca gcttcgcata aaaccaaacg ctctcgaact gattaaagaa tatgtgaata
     1021 acggtggtgg gttattgatg attggcggct atctctcatt catgggaatt gaagcaaaag
     1081 ccaattataa aaatacagtt ctggctgatg tgctcccggt tacgatgtta gatggtgacg
     1141 atcgtgtcga aaaaccagag ggtgttattg cacagccatc acaacctgac catccggtta
     1201 ttaaagggtt ctctgaatat ccttttttcc taggttataa ccgggcgatc gctaaagaaa
     1261 atgctgaggt ggtattaaca ataaacaacg atccattact ggtctttggt aactatcaca
     1321 acgggaaaat agcctgcttt atgagtgact gttcaccgca ctggggtaca cagcagttta
     1381 tgtcctggcc gttctatacc gcgttgtggg taaatatact gactcatatt gcaagataat
     1441 aataataatg gggtaacacc agttactcca tttactataa tagaaggatt gatgtatgaa
     1501 aaagaataag tactcaactc ccttatgggt gcttgccacg attcttgcgg gtatgctctc
     1561 ccctatgcaa tcagcggtga atggtcaatt aggtcactgg ttacaggacg gtaatgcatg
     1621 cgcagttatc tcctttgcca gtggtttagt cgtgatgttt tttattatta tggcgcgaaa
     1681 agaaatccgg caacaatttg cggcaattcc ttctctgatt aaaaaccgaa aagttcctct
     1741 ctggaactgg tttgccggtc tctgtggagc gatggtggta ttttctgaag gcgcatcagc
     1801 cagcgcactc ggggtcgcaa cgtttcagac cgcactgatc tccgcgctat tgctgtctgg
     1861 tttactttgc gatcgctttg gtatcggtgt cgacgagaaa aaatacttta ccccttatcg
     1921 catcatcggt gcattatttg cggttattgc tacgattttt gttgtgtcac cgcagtggca
     1981 ttctacctct ttcattttac ttgccattct tccgttctta gctggtttgc ttgcaggatg
     2041 gcagcccgct ggaaatgcaa aagtagctga agctacggga tccatgctgg tttcaatcac
     2101 ctggaatttc atcgttggat tttgtgtttt aggcacagcg ctagctattc gtgtcgcatt
     2161 agggcacgtc actgttcaat taccagatgt ctggtggatg tatcttggcg gcccgcttgg
     2221 actaatgtct atcggtttaa tggccatttt ggtcaggggt ctggggttac tgatgctggg
     2281 ggttgcttca acggcgggcc agttactggg atcagtattg attgacgtat taattccatc
     2341 gctgggcaat accgtttatc tggtcacgat catcggaaca ctgtttgcac tggtcggtgc
     2401 aattgttacc actattccgg aatacagagc ctcgaaaaca atgaagaaaa tggaggtata
     2461 atcatgaaag attatctaca aactgtgacg ggccccgtcg cccgtgaaga tatggggcta
     2521 acacttcctc atgaacatct gttcaacgat ctttcctctg ttgtggatgc tccttgctat
     2581 cctttttcac aacagcttgt tgataaaaaa gtcacggcag aaatccagtg ggcattaaaa
     2641 cacgatcctt attgctgcgc tgataatatg gaccgcaaac ctattgaaga tgtgatcttc
     2701 gaaattaata actttatctc gctcggtggt cgcaccattg ttgatgccac agggtctgag
     2761 tctatcggac gagatgcgca ggcattacga gaagtcgcat taaaaacagg tctgaatatt
     2821 gttgcttcct ccggtcccta cctggaaaaa tttgaaagcc agagaattca taaaacggtt
     2881 gatgaactag cgacaacgat cgataaagaa ttgaatcaag ggattggcga tacggatatt
     2941 cgtgccggaa tgatcggtga aataggtgtc tcaccgacat ttaccgaagc cgagcataac
     3001 agcttgcggg ctgcctcgct ggcacagatt aacaatcctc atgtggcgat gaatattcac
     3061 atgccgggct ggcttcgtcg cggtgatgaa gtactcgaca ttgtgttagg tgaaatgggc
     3121 gtctcgccaa ataaagtctc tctcgcacac tcggatccgt caggaaaaga cgtggcgtat
     3181 cagcggaaaa tgcttgataa aggtgtctgg ctggaattcg acatgattgg cctagacatt
     3241 accttcccga aagagggaat agcgccaggg gtgcaggaga ctgccgatgc cgtcgctcat
     3301 ctcattgagt tgggatacgc cgatcagctt gttctcagcc acgatgtctt ccttaaacaa
     3361 atgtgggcta aaaatggcgg taatggctgg ggatttgttc cagatgtttt tctggcctat
     3421 ctggcggagc gcggcgtcga taaaacgatc ctcaaaaaac tctgtatcga taatcccgga
     3481 cgattattga ccgcataaaa ccattatccc ccggcacatt gcgccggggg atgtcaatta
     3541 ataccctgcc gttaaatcat ccaccaagcg cgagtcggat gcgccgtaca actcaccatc
     3601 cgacccaacc ataatgcttt gcgtgctgcc catcgcctca ttcagcgcca ctttctgacc
     3661 ttttgcttcc agcagcttga gcgtatccgg actaaaccct tttcaacacg cagctcgtcc
     3721 ggcaaccact gatggtggaa acgaggcgca ttggtcgctt cggcaacatt cattccacaa
     3781 ttgatgctgt tcaccaccat ttgcggcacg gtagtgatga tccagctacc ggtaacaaag
     3841 tgagtacata aaggccatac tgtcacgaac agtgcaaaag tggctcttcc tcaacctagg
     3901 tgaagatttt catgccagtg gactcattac cctccgccta agtagttcaa gtccggctcg
     3961 accatacatc tggcgtatca gcaccttcag gcgatttaca tgaccttcga caacaccagt
     4021 actccagcga ctgctgattg cctcacatat agccgcagca tcagcttcca tccccgcagc
     4081 tacgcaccga agttctgctg agccgctttc gtggacccga gtgaaccagc tgcttagctg
     4141 tgatttattt taggttttca gaatacggta gaactcgagt accagttgct gcgctatttt
     4201 caactccggt tctttttcac acatcagact aataaatcgg aacgcataat tttcttcacc
     4261 cctgattatt ctccagggca tcaaccagcg gcttacccgg gacactgaag gaagtcttgc
     4321 ggcagtagta accggtggga tccagccttt atgccatttg gcaacagcat ccctgacgat
     4381 ggtttcactg cctgtaaccc ccctggccac catttcccgc catatccggc cggagttata
     4441 attaccacat tctcgttgct cttttaacca ctccctccat gggtccagaa gccctcgctt
     4501 tggaggccta gttgacattt cagggaatgt tcctgaaccc acccagcgac tgactgtcac
     4561 tcgcgacagg cctgtaatac gggatatttc cctgaaacta catcccttgt tatgcagggc
     4621 cataacctct gtccaacgct gatgccgttt tttgcggatg tgctgtttaa ggcgttccag
     4681 acgacggagc gatgctacag gcacagatat ttctggctca ggtgatttct tcagtgataa
     4741 ctctctgaca acaagacgta tcagaggcat atatctgtac atcattcgtt caggctcatc
     4801 accaatattt tttagcaggt gccatcgatc ggccacctgt ctggcctgag gtgcaccttc
     4861 acgtgctgct gtcgcataga ctccactgcg atcacgcgag acaacctgta tttccggata
     4921 ttttctgaac caggtcgcca gcgtacgctg atcacggccg ggaagcagga cgaggggacg
     4981 gtgagtatca ggattgacta ttaacatacc gcagcagtgg ccccgatgcc acgcccactc
     5041 atcaataccg acatgaggcg cgcctgactg tttcgtctcc ggggtattga taagcctgcg
     5101 aagaagagta tcagcactgc aacggagacc cactgccgtt gcagcccgtt ttccagcctc
     5161 acctccagct attaatccca gttgacgttg ttaattttgt aacgcctgtg aagactgctg
     5221 gtgtgaactg gcgaagggag caagcgactc ggcaaaaatt ttacgtgaac aagccgggtt
     5281 acggcagtac cagtggcgga cggaaaatac aagccatagc gtctgccctg agcagggtag
     5341 atgctgtatc cggcgccgac gacaactatg aacagagtcg ctacgcttaa agcattcagg
     5401 acattgtgct gttttgcgtt ttccgtggag atggagggtt ataccgtcag agctaataat
     5461 ttgtcgactg catcgccatc cgcaaggtaa ctgtagaagt gtcttaagag agtgcattgc
     5521 gttgcccata acttaaaaca gcttagatcc ttgttcgcca gaaagtttca tccattttct
     5581 tccacccagg ttgcggaaga accacttttc atccgtcgtt gacagatgtg aagtatgcgg
     5641 gagaaaaagt gaatatctaa ctgtaataac tcagaccggt acgagtactc gggacgtcta
     5701 caatcattca gggcaaagtg ctaaaatttc tctatcccgg gatgccccta ccaacgcagc
     5761 actcccggag atatcattac agctctgcaa ctgatttccg cgactgaacc gctcagttcc
     5821 tatccttc
//
LOCUS       DAZGCS010000198         5716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGCS010000198 DAZGCS010000000
VERSION     DAZGCS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.201"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..1129
                     /locus_tag="ACHK01_004798"
     CDS             32..1129
                     /locus_tag="ACHK01_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8099594.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1319..1702)
                     /gene="quuD"
                     /locus_tag="ACHK01_004799"
     CDS             complement(1319..1702)
                     /gene="quuD"
                     /locus_tag="ACHK01_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO8099595.1"
                     /translation="MCDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1720..2709)
                     /locus_tag="ACHK01_004800"
     CDS             complement(1720..2709)
                     /locus_tag="ACHK01_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099596.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2717..3526)
                     /locus_tag="ACHK01_004801"
     CDS             complement(2717..3526)
                     /locus_tag="ACHK01_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO8099597.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3546..3935)
                     /locus_tag="ACHK01_004802"
     CDS             complement(3546..3935)
                     /locus_tag="ACHK01_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8099598.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            complement(3932..4258)
                     /locus_tag="ACHK01_004803"
     CDS             complement(3932..4258)
                     /locus_tag="ACHK01_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO8099599.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(4255..4908)
                     /locus_tag="ACHK01_004804"
     CDS             complement(4255..4908)
                     /locus_tag="ACHK01_004804"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO8099600.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(4908..5402)
                     /gene="yfdN"
                     /locus_tag="ACHK01_004805"
     CDS             complement(4908..5402)
                     /gene="yfdN"
                     /locus_tag="ACHK01_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8099601.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(5399..>5716)
                     /locus_tag="ACHK01_004806"
     CDS             complement(5399..>5716)
                     /locus_tag="ACHK01_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HFO8099602.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
BASE COUNT         1378 a         1588 c         1329 g         1421 t
ORIGIN      
        1 tttaataata gttcaaaaaa aagggctcac gatgaaaaaa ttaacagtgg caatttctgc
       61 tgtagctgca tcagtactga tggcgatgtc tgctcaggca gctgaaattt ataataaaga
      121 cagtaacaag ctggatctgt acgggaaagt taatgccaag cactactttt cctctaacga
      181 tgcagatgat ggtgatacta cttatgttcg tctgggcttc aaaggcgaaa cccaaatcaa
      241 cgatcagctg actggtttcg gtcagtggga atatgaattc aaaggcaacc gcgctgaatc
      301 tcaaggttcc tccaaagaca aaacccgtct tgcatttgca ggcctgaaat tcggtgacta
      361 cggctcaatc gattacggcc gtaactacgg tgtagcatac gacatcggtg cgtggactga
      421 cgttctgcca gaattcggtg gcgatacctg gacccaaaca gatgtgttca tgactggtcg
      481 caccactggt gttgcaacct atcgtaacaa cgacttcttt ggtctggttg atggtctgaa
      541 ctttgctgct cagtaccaag gcaaaaacga tcgtagcgat ttcgataact acaccgaagg
      601 taacggtgat ggcttcggtt tctctgctac ctatgaatac gaaggattcg gtatcggtgc
      661 aacttatgcg aaatctgatc gtaccgacac tcaagttaat gcagggaaag ttcttcctga
      721 agtatttgct tccggtaaaa atgcagaagt ttgggccgca ggtctgaaat atgacgctaa
      781 caacatttac ctggccacta cctattctga aacccagaat atgactgtat ttgctgatca
      841 cttcgttgct aataaagctc aaaacttcga agctgttgca caatatcagt tcgatttcgg
      901 tctgcgtccg tccgttgctt acctgcaatc taaaggtaag gatcttggag tatggggcga
      961 tcaggactta gtcaaatatg ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc
     1021 tactttcgtt gattacaaaa tcaacctgct tgacaaaaat gacttcacta aagcactcgg
     1081 tgtaagcact gatgacatcg ttgctgtagg tctggtttac cagttctaat ctgattacga
     1141 aaaagatatg ttgcgggagg cgttgcctcc ccaacatata agtggctccc tcaagccact
     1201 tcctttagga gcacaacctt gcttctaact atataaacct tctgttatat attacccttt
     1261 atttttgggg gcgttgcaac gccccatttt taataacttt cagtaaacaa ttggcatatt
     1321 aattagagtt attaacaacg atatccatct ctaaccggat atctaatgcc attaacatcc
     1381 cttcaattat gccctcagcc ttctgtaacc ttttcccgat ataaccatca gagcagcaat
     1441 gcttacctgc cagtgacatg aatgtcatac cgactacata ataatctact aataaatcgt
     1501 gcaaatcgct gttgttcttt ttcagacggg ccatgcaccc gcaaatgatc atcgcgtcat
     1561 cgtcacaaca ttgcgggcga gattttactt ttgaaggaat taatccctta aaaccggcgg
     1621 caatggacga ccaggtcaca tcttcatgat tattagccgc ccacgctccc caacgttcaa
     1681 gaaccatctg aatatcacac attaactttc tccacaaaat caggccagca caccaatcgc
     1741 cagtgcgcga tcgataaaac gaaatatcag ctccagctgg gagccatact tctcttcaaa
     1801 tgccacggta tccgcatgca gttcgtcgtg gtgttttctg cacaaaggca gcacaaaaag
     1861 gtcatgcgct tttgtaccca ttccaccctg accatggcct atcagatgat gcggatcatc
     1921 agctggcttt ccacaacatg cacacggctg tgtcttaacc cagcgcgtgt acttttcatt
     1981 aacccagcgg cgacgttttg ggcgtaacat aaaagactcc ggcgactccg gatccacttt
     2041 cagcgccagc acctttttcg ccttatcctg gatgatgctg gtggcaggaa ccgaaggcac
     2101 aaggtcactt tcccgggtaa cagacggcac aacaggcttc ggtaatctca gtgccttacg
     2161 ggctgcactt tccggtaagg catccgccag atcattacga atcagccacc agcacagttc
     2221 cggcattgtc acaacgtgac tgtcatcaaa accgagatcc cgacgcacaa cagacaacac
     2281 ccagcgggca cagttatccg ttgccattga ttccagccgt tccgtgaact gatcgcgcag
     2341 ctggttatcg cagtgccagc acagacggat tgcacccgga gcgtgtcgca ttgtggtcat
     2401 gttctcgctg tgccagtcgg aatgaggcca ctggcagcct ttttcacgaa gtaaccagct
     2461 ttcaagacat tccacgccac cagcacgacg gatcactgcc tcattgcgga acacggcccg
     2521 aacggcagga tcatccgcca gcggttgtga tgccgccgga acggcaccac tggcgaaaga
     2581 tgaataacgc tccggctcag gctccagcag gacacgcccc tgcataaaca ggggcatcag
     2641 ctctgaacct ggcctgaaca atacgatccc catacgcggg gcaatttcag gggtcagtag
     2701 tgctctcacg gtcacctcaa tgaacggtat cgagcagctt taacagctca gggaatcggg
     2761 attcgaagaa atgcggctgc gtctcgcgcg gatttgcggg actggtgatg ttcttgccga
     2821 acatgcaacc tttcgctgtc agcgaccaga attttttgat gttgttaatc gctgtacggc
     2881 tgtatcgttc gcgctgctcg acgatcccca gtttcaccat ctggtgatat gcctgattag
     2941 ccgtcaggcg tataccatac tgtttcagca gtgcactcag tgacagtgtc gggcgacttg
     3001 agccatcgtg tgcatcagca ggagcatcaa tggcatagcg cggtgccaga ttcggtaagc
     3061 caacagcctc ctggagtttc tgacaggcac caagcactga agagttagac aggtttaact
     3121 cccggcgcat aaagtccagc aggatcacgc cagcctgcat cttgtcagca gcctgtccgg
     3181 ataatttttc cggtgcgctg gttaccatat cgaaagtacg gatcaccttc agatggaatg
     3241 acgggctgat ccacattgca taggcataca ccagttcctt gcagacatac gttccccgtt
     3301 catttccccc atgaatcaca ctcaccgggt caacacccaa attctgggtg ttggtcaatt
     3361 catgaacaag ctcaacagtt tgttggctgg aaagaaactt tcccggctcc ttggttctgg
     3421 catttgcacc agatgctact gctgcgcgat gcagatcgtt caggctgtaa cgcccataag
     3481 catcacgacg aacttcaata ccatcaatga ccatcagatt attcatactt cgtttctcct
     3541 cttgatcagg cggctgcacc cgccgttttc tcgtacttac tgatagtgat ctcgaccttc
     3601 cctttcggga taaccggtcc ccactccacc agcattcttt tcacctgact gtcgtcttcc
     3661 cacacacccg catgggtcag ggcgtcaaac agcgccttgt tatagttgtc cagatcgcgg
     3721 atccggttat ccggaggaaa caacacgatc tccactgaag caggtgccga cgttggtttc
     3781 ggcagacgac gtaactgctc aactattgct gcgcacgccg cgctctggaa tttgcgcccc
     3841 gctgcgctta tcagactctt accagcaaac gcccctttgt tggggtgtcg ccagtacgtg
     3901 ttcacgctgg gagggaaagg caagatcagc ttcatacttt caggcccctc tcatgtaacc
     3961 agtgggctgc acgcagcctg gcgttttcct caccggcaag cagtgcgcgg ataatcccga
     4021 ccgcctcgct gtcgtcgtcc ttcaccgcgg tatgaagagt tatcccccgg gccacgccac
     4081 gctttatcgt gatgacgcct tttttctcca gtgcgcgaag atgctccacc gctgcattca
     4141 ccgaacggta tcccagcatg gttgccacct cctgattggt tggcggaaag ccacgttctt
     4201 gctggtaaga aatcagcata tccagcacct gctgctggca ttgagttaac gtcgtcatgc
     4261 cgccatctcc ctgaccagtt tttccgcctg ctggcgaacc tgcgccagaa aggcctcacc
     4321 acatgcctca agttcatcgc gcccgatgta gctgattgcc ggtcccttcc aggtcttgtc
     4381 gaaaacagca atagcaccag cgaagaaagc gcctgtcggc acctgcttct catccttcgg
     4441 gataaaccag gcaggcagtt caaaaccaat acgcccgcga ataaaagcaa tatggtccgc
     4501 atcttccggc caccacactt cgctggtggc agctttgatc aggaaaacat agcgcccgcc
     4561 cttatcacgc atggcactgg catgtttcat gatgtaacgc atgccggtga tgtattgccc
     4621 ctcatgctga ctggcgcggc tgtatggggg attaccaaag gcagcacctt taagctccgc
     4681 aagacgttct gaccagtcat gcgccagcgc gttatcttcc gccgtgtaat acgcggcaca
     4741 tttggcgtta tcaccgtcag taaacagatc cagaacaaac gggccaaaca gggtgttaat
     4801 tccccagaaa atgttgtccg gcgtgcgcca ctgatcgccc acttccttca gttcatgggc
     4861 tggtttgttc cgcagttcca ccagcgcctg gcaatattta ttactcatta agcccccacg
     4921 taattccctg acagatacca ctcttcaccc gatgcagcgc gcttgctgct tttccgtaag
     4981 caccgctcac gacgcgccag aaaattgttt cgttctggct gggagtggct ttcacggaat
     5041 gccgccatcc acaccgttgc agcacgacgg tataagcccc tggactccag ttcttcagcc
     5101 tggcgggtca ggcacaaaat cacccgtgga tcgttagtgc cgacatagaa attgcgcaca
     5161 ggtctggttt ctcgaactgg ttgtggttcc ggttcctgcg ctctctcagt caggcgcggg
     5221 aaatgtctgc gtgtatctcc ttcacaacgg tgagccacac gcccactctg acgtaacttg
     5281 cttgctgact gcagaacgcg ctgccgtgag taacctgcaa aagcatccgc aatgtctccg
     5341 gaagtacatc ccggatgggc ttcaatgaat ttctgaactt cattcaaaag actcatgatc
     5401 accccctgaa tcctgccggg atctggctgt agtccacgtt gtcgtaactg gatttgaagt
     5461 acgggtcttc gcgtttttcg gtgtacgtgc tgacggacgg tgataagcgc agggaaagct
     5521 catcccattt ttcccgcaac ttcgacgggc tgagcacgtt acggcaccag aacggatcgc
     5581 ggctgacgcg gctgtacatc tcgcagattt gtttgtgagt acgaccatcc tgcacacaca
     5641 tcaggcgaat ttcgtttgcc caggctgtcc agttcggttc tttgggacga accacctcgc
     5701 cgtcacattc ggcggc
//
LOCUS       DAZGCS010000199         5669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGCS010000199 DAZGCS010000000
VERSION     DAZGCS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.202"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..585
                     /gene="fimA"
                     /locus_tag="ACHK01_004807"
     CDS             43..585
                     /gene="fimA"
                     /locus_tag="ACHK01_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8099603.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            805..1497
                     /gene="fimC"
                     /locus_tag="ACHK01_004808"
     CDS             805..1497
                     /gene="fimC"
                     /locus_tag="ACHK01_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8099604.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1528..4136
                     /gene="sfmD"
                     /locus_tag="ACHK01_004809"
                     /pseudo
     CDS             1528..4136
                     /gene="sfmD"
                     /locus_tag="ACHK01_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4149..5156
                     /gene="fimH"
                     /locus_tag="ACHK01_004810"
     CDS             4149..5156
                     /gene="fimH"
                     /locus_tag="ACHK01_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8099605.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5167..>5669
                     /gene="sfmF"
                     /locus_tag="ACHK01_004811"
     CDS             5167..>5669
                     /gene="sfmF"
                     /locus_tag="ACHK01_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO8099606.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFM"
BASE COUNT         1484 a         1231 c         1369 g         1585 t
ORIGIN      
        1 tatacggatg tggagtcgat aaatgagatt gaaggaatat atatgaaatt aagatttatt
       61 tcgtctgcgc tggctgccgc actattcgcc gctacgggta gttatgctgc cgttgtagat
      121 ggcggtacaa ttcactttga aggcgaactg gtgaatgctg cctgttcagt gaatactgac
      181 tcggcagacc aggttgtcac actcggtcaa tatcgtaccg atattttcaa tgctgttggt
      241 aatacctctg cattaattcc attcaccatt cagttgaacg actgcgatcc tgttgttgcc
      301 gctaatgctg ccgttgcatt ttctggtcag gctgatgcaa tcaatgataa tttattggcc
      361 attgcatcca gtaccaatac aacaacagca acgggtgtcg gtattgaaat acttgataat
      421 acatccgcaa ttctcaaacc tgatgggaat agcttctcaa ccaaccagaa cttgatcccc
      481 ggaaccaacg ttcttcattt ttctgcacgt tataaaggca ccggtacaag tgcatcagca
      541 gggcaagcaa atgctgacgc gacttttatt atgagatacg aataatcaaa accacgttgt
      601 tttgaattat atatcacgtc ttataacaaa gtaatgtacc ggttgtctga agcggtatgg
      661 tggcaatgta aatcgaaatc atgttcactt tgtatcatgc cgctttatta aatgaaaagg
      721 gaatgatgtg ttgtaagaaa ccaaagcaat catttcttta tatttcttat ttttgccgtc
      781 aggaatacac aaggcgtatt aactatgatg actaaaataa agttattgat gctcattata
      841 ttttatttaa tcatttcggc cagcgcccat gctgccggag ggatcgcatt aggtgccacg
      901 cgtattattt atcccgctga tgctaaacag actgcggtat ggattagaaa tagccatacc
      961 aatgagcgtt ttctggtcaa ttcgtggatt gaaaacagca gcggtgtaaa agaaaagtca
     1021 ttcatcatta caccgccact gtttgttagt gaacccaaaa gcgaaaatac tttgcgtatt
     1081 atttacaccg gtccaccgct ggcagcagat cgtgagtctc tgttctggat gaatgttaag
     1141 acgatccctt cggtagataa aaatgcattg aacggcagga atgttttgca actggcgatt
     1201 ttatcgcgca tgaaattatt tctccgtcca attcaattac aagaattacc cgcagaagcg
     1261 ccggacacac tcaagttttc gcgatccggt aactatatca atgttcataa tccatcacct
     1321 ttttatgtca ccctcgttaa cctgcaagtg ggcagccaaa agttggggaa tgctatggcg
     1381 gcacccagag ttagttcaca aattccctta ccctcaggag tgcagggaaa gctgaaattt
     1441 cagaccgtta atgattatgg ttcagtaacc ccggtcagag aagtgaactt aaactaaccg
     1501 aatcatctga caatatcaga gctaattatg aaaataccca ctactacgga tattccgcag
     1561 aggtatacct ggtgtctggc cggaatttgt tattcatctc ttgccatttt actctccttt
     1621 ttaagctatg cggaaagtta tttcaacccg gcatttttat tagagaatgg cacatccgtt
     1681 gctgatttat cgcgctttga gagaggcaat catcaacctg cgggagtgta tcgggtggat
     1741 ctctggcgta atgatgagtt cattggttca caggatatcg tatttgaatc gacaacagaa
     1801 aatacaggtg ataaatcagg tgggttaatg ccctgtttta accaggtact ccttgaacga
     1861 attggcctta atagcagtgc atttcccgag ttagcccagc agcaaaacaa taaatgcatc
     1921 aatttactga aagctgtacc tgatgccaca attaactttg attttgcagc gatgcgcctg
     1981 aacatcacta ttcctcagat aacgttgttg agtagcgctc acggttacat tccgcctgaa
     2041 gagtgggatg aaggtattcc tgctttactc ctgaattata atttcaccgg taacagaggt
     2101 aatggtaacg atagctattt ttttagtgag ctcagcggga ttaatattgg cccgtggcgt
     2161 ttacgcaaca atggttcctg gaactatttt cgcggaaatg gatatcattc agaacagtgg
     2221 aataatattg gcacctgggt acagcgcgcc attattccgc tgaaaagtga actggtaatg
     2281 ggagacggca atacaggaag tgatattttc gatggtgttg gatttcgtgg tgtacggctt
     2341 tattcttccg ataatatgta tcctgatagc cagcaagggt ttgccccaac ggtacgtggg
     2401 attgcccgta cggcggccca gctaacgatt cggcaaaatg gttttattat ctatcaaagc
     2461 tatgtttccc ccggcgcttt tgaaattaca gatttgcacc cgacatcttc aaatggcgat
     2521 ctggacgtca ccatcgacga gcgcgatggt aatcagcaga attacactat cccgtattca
     2581 acagtgccaa ttttacaacg cgaagggcgt ttcaaatttg acctgacggc gggcgatttt
     2641 cgtagcggta atagtcagca atcatcgcct ttcttttttc agggcacggc actcggcggt
     2701 ttaccacagg aatttactgc ctacggcggg acgcaattat ctgccaatta caccgccttt
     2761 ttattaggac tggggcgcaa tctcggaaac tggggggcag tgtcgctgga tgtgacccat
     2821 gcgcgcagtc agttagccga cgacagtcgt catgaggggg attccattcg cttcctctat
     2881 gcgaaatcga tgaacacctt cggcaccaat tttcagttaa tgggttaccg ctattcgaca
     2941 caaggttttt atacccttga tgatgttgcg tatcgtcgaa tggaggggta cgaatatgat
     3001 tacgattacg acggtgagca tcgggatgaa ccgataatcg tgaattacca caatttacgc
     3061 tttagccgta aagaccgttt gcagttaaat atttcacaat cacttaatga ctttggctcg
     3121 ctttatatct ctggtaccca tcaaaaatac tggaatactt cggattcaga tacctggtat
     3181 caggtggggt ataccagcag ctgggttggc atcagttatt cactctcatt ttcgtggaat
     3241 gaatctgtag ggatccccga taacgaacgt attgtcggac ttaatgtttc agtgcctttc
     3301 aatgttctga ccaaacgtcg ctacacccgg gaaaatgcgc tcgaccgcgc ttatgcctcc
     3361 tttaacgcca accgtaacag caacgggcaa aatagctggc tggcaggtgt aggtgggacc
     3421 ttactggaag gccacaacct gagttatcac gtaagccagg gtgatacctc gaataatggg
     3481 tatacgggca gcgccacggc aaactggcag gccgcttacg ctacgctggg ggtcgggtat
     3541 aactacgacc gcgatcaaca tgacgttaac tggcagctgt ctggcggtgt ggtcgggcat
     3601 gaaaatggca taacgctgag ccagccttta ggggatacca atgttttgat taaagcgcct
     3661 ggcgcaggcg gtgtacgcat tgaaaatcaa actggcattt taaccgactg gcgcggctat
     3721 gcggtgatgc cgtatgccac ggtttatcgg tataaccgta tcgcgcttga taccaatacg
     3781 atggggaatt ccatcgatgt tgaaaaaaat attagcagcg ttgtgccgac gcaaggcgcg
     3841 ttggttcgtg ccaattttga tacccgcata ggcgtgcggg cgctcattac cgttacccag
     3901 ggcggaaaac cggtgccgtt tggatcactg gtacgggaaa acagtatcgg aataaccagt
     3961 atgtgggtga tgacgggcaa gtttatttaa gtggtgcgcc attgtctggt gaattactgg
     4021 ttcagtgggg agacggcgcg aactcacgct gcattgcgca ctatgtattg ccgaagcaaa
     4081 gcttacagca agccgtcact gttatttcgg cagtttgcac acatcctggc tcataaagga
     4141 aattatcaat gaagataatc tgcagattat tattggcgat ggcatgtttg tgtctggcaa
     4201 acatatcctg ggctactgtt tgtgcaaata gtactggcgt agcagaagat gaacactatg
     4261 atctctcaaa tatctttaat agcaccaata accagccagg gcagattgtt gttttaccgg
     4321 aaaaatccgg ctgggtaggt gtctcagcaa tttgtccacc cggtacgctg gtgaattata
     4381 cataccgtag ttatgtcacc aactttattg ttcaggaaac tatcgataat tataaatata
     4441 tgcaattaaa tgattatcta ttaggtgcga tgagtctggt tgatagtgtg atggatattc
     4501 agttcccccc gcaaaattat attctgatgg gaacagatcc taacgtttcg caaaaccttc
     4561 cattcggggt gatggattct cgtttaatat ttcgtttaaa ggttattcgt ccctttatta
     4621 acatggtgga gatccccaga caggtgatgt ttaccgtgta tgtgacatca acgccttacg
     4681 atccgttggt tacacctgtt tataccatta gttttggtgg ccgggttgaa gtaccgcaaa
     4741 actgcgaatt aaatgccggg cagattgttg aatttgattt tggtgatatc ggcgcatcgt
     4801 tatttagtgc ggcagggccg ggtaatcgac ctgctggtgt catgccgcaa accaagagca
     4861 ttgcggtcaa atgtacgaat gttgctgcgc aggcttattt aacaatgcgt ctggaagcca
     4921 gtgccgtttc tggtcaggcg atggtgtcgg acaatcagga tttaggtttt attgtcgccg
     4981 atcagaacga tacgccgatc acgcctaacg atctcaatag cgttattcct ttccgtctgg
     5041 atgcagctgc ggcagccaat gtcacacttc gcgcctggcc tatcagtatt accggtcaaa
     5101 aaccgaccga agggccgttt agcgcgctgg ggtatttacg cgtcgattat caatgaggta
     5161 cggagaatga gaagagcact ctttagctgt ttctgcgggt tactgtggat ttccagtgga
     5221 tgggcagctg accctttggg aacgattaat atcaatttgc acggtaacgt tgttgatttc
     5281 tcctgtaccg taaacactgc ggatattgat aagacggtag atttaggcag atggcctacg
     5341 acacaattac tgaacgctgg cgataccacg gcactcgtcc cttttagcct gcggctggag
     5401 ggatgtcctc cgggttcagt tgcgatttta tttacgggaa cgccggcatc cgataccaac
     5461 ctgctggctc tggatgatcc cgcaatggcg caaaccatcg ccatcgaatt acgtaatagc
     5521 gatcgctccc ggctcgcact gggagcggcg agcccgactg aggaagtaga tgcaaatggc
     5581 aatgtcacac taaacttttt tgccaattat cgagcgttag ccagcggtgt tcggccaggt
     5641 gtggcgaaag cggatgcgat atttatgat
//
LOCUS       DAZGCS010000200         5593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGCS010000200 DAZGCS010000000
VERSION     DAZGCS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.203"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..733
                     /locus_tag="ACHK01_004812"
     CDS             41..733
                     /locus_tag="ACHK01_004812"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8099607.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEK
                     AITDFMTARGYH"
     gene            complement(782..2281)
                     /gene="pitB"
                     /locus_tag="ACHK01_004813"
     CDS             complement(782..2281)
                     /gene="pitB"
                     /locus_tag="ACHK01_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO8099608.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2573..4432)
                     /gene="gss"
                     /locus_tag="ACHK01_004814"
     CDS             complement(2573..4432)
                     /gene="gss"
                     /locus_tag="ACHK01_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO8099609.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4637..5503
                     /gene="yghU"
                     /locus_tag="ACHK01_004815"
     CDS             4637..5503
                     /gene="yghU"
                     /locus_tag="ACHK01_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO8099610.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT         1420 a         1443 c         1373 g         1357 t
ORIGIN      
        1 taaatatttt taatggatat ttaaattaaa ggatatattc atgcagtcaa taacacctcc
       61 attaattgcc gttattggta gcgatggttc aggcaagtca acggtgtgtg aacatcttat
      121 taccgttgtc gaaaaatatg gtgctgccga aagagttcat ttaggaaaac aggccggaaa
      181 tgtcggtcgt gcagtgacaa aattaccgtt gatgggaaaa tccttacata aaacaattga
      241 acgaaatcag gtgaaaacag caaaaaaatt gcctggacca gttccggcgc tggtaattac
      301 agcgtttgtc gcccgtcgct tactgcgctt tcgtcatatg cttgcctgtc gtcgtcgcgg
      361 gttaattgtt ctaaccgacc gttatcctca ggaccaaatt cctggcgctt acgatggtac
      421 ggtgttccca cctaacgttg aaggtggtcg ttttgtctca tggctggcaa gccaggaacg
      481 taaagcgttt cactggatgg cgagccataa gcctgatctg gtcatcaaac tcaatgttga
      541 ccttgaagtt gcctgtgcac gtaaacccga ccataaacgg gaatcgctgg cgaggaagat
      601 tgccataacg ccacagttaa cctttggtgg tgcacaactg gttgatatcg atgccaatca
      661 gccactggaa caggtgttgg ttgatgtaga aaaagcgatt acggatttta tgaccgcgcg
      721 tggttatcac tagtcaaaaa tggaaatgcc cgatcgccag gaccgggcat tttcaggaag
      781 gttaaatcaa ctgcaatgct atccagtaca gcccaccaga aagaaaaatt gccgccggta
      841 aagtaaacac ccacgccatc aggatgctgg ttaccgtttt acgctgtaac ccaccgccgt
      901 ccaccaccat cgtccctgct accgcagacg agaggacgtg tgttgtggag acaggcatcc
      961 caatataact ggcaaggccg atagacactg ccgccgtcat ttgtgccgcc atgccttgcg
     1021 catacgtcat gccgcgctta ccaatcttct caccgatggt catcgctaca cgacgccagc
     1081 caatcatggt gccaatgccg agcgccagtg ctaccgccat gatgatccac accggagcgt
     1141 actcaatggt gcttaacata tcgctgcgca gttttttcag caggttctgg tcttctttac
     1201 tgacgcctgg cagtttcgcc agcttcgcgg aggtatcaga gatgcacagc ataatgcggc
     1261 gcagctggct gcgctgactc acggttaacg gctcgtaact ttccatattg cctggcagca
     1321 tcgttttaac gcgcgcaata gcatcaaagg tatttgccgg atgacagtga aactctgtta
     1381 cttgcgtgcc atcagtcgat gctgcaggca atggaggttc catcgcaatc aacttctgcg
     1441 gcagttcagg atgctgttgc aggtagtgtt cgaagttggt aacggcatcg cgggtacggg
     1501 taatttcata gccggacgca ttcatattga cgacgaagcc agcaggggca atccccacca
     1561 gtaccagcat taccaggccg atccctttct gtccgtcgtt cgcgccgtgc gaaaacgcca
     1621 cgcccgcagc ggaaacaatc agcgcaatac gcgtccagaa tggcggttta cgtttgcctt
     1681 ttttcttttt gcgatcttcc ggaatgcggt gaatacggtc acgctttttc gtcccgctcc
     1741 agtagcgtcg cagcaggaat atcaggcctc ccgcaatgac caggccgacg ataggggaaa
     1801 caatcagcga ggagaaaatt ttggtcactt cacgcaggtt taacgcatcc atcaccgatg
     1861 agccggttaa cagcgcgttg gttaaaccga tgccgataat cgcaccaatc aaggtgtgcg
     1921 aactggaggc cggtaaaccg aagaaccacg ttcccaggtt ccagataatc gccgccagca
     1981 gcatggaaaa gaccatcgcc aggccgtggg ttgaccccat attcagcaac aaatcggttg
     2041 gcaacatatg gacaatggca taggcaacgc taagtccgcc caataacacg ccaaaaaagt
     2101 taaaaaatgc cgccatcacc acagcaagtt gtggttgcat ggcacgagta taaataacgg
     2161 ttgccaccgc attcgccgtg tcatgaaaac cattgattgc ttcgtagaac aacacaaatg
     2221 ccagagcaag caataacaaa agccctgtgt atatatcaag gccaacaaat aaatttagca
     2281 tatattagat tacgccattt tgaatttacg aacggacgca ttatcagtga ctttaacggc
     2341 atgggcaaag tgaaatatca ttttttaacg ataaaaagag tgttatttct tgtgtatcag
     2401 acaataagca ttcattcaaa acctttagaa ataatcaaaa cacaggtatt ccactggtta
     2461 atatattcac atatgaaatg aataaatatt ggaatatata aatattgaat attttgatta
     2521 atccccgtac tgattattct tcataatcag tacggggttg ctacaacatg tattactttt
     2581 tcaccacaat taacggttca atatcactct cttttttgat gaccagtgat tcatcaccgc
     2641 gcaaacacgt cccaccgtag ttgccgccaa cggtgaaggt acatacctga atgtatttac
     2701 cgtccacttt cggcaaacac cacagttgct gatagatgtt tttctgctcg gcaaatttac
     2761 cgctggtttt gtccagcacc tcttcatgat ggctgacgag gtcgatattg ctgccacagc
     2821 gaccggcgat cggtttcact gcgtaacctg ttttcaccag ttcatcatta acagtgaaat
     2881 cggtatccag caggtaacga tggtgcggga acagcgacca gaggatcggc agaatcgctt
     2941 tgttgccggg gatcaccgtc cacagcggct caaagaccag cacttccggg cgcagcaata
     3001 cgtcgataag acgcacttcg ttttgcggat gaccggtacg gattggcacc gcagcaaact
     3061 cacggtcgct aacttcacga atctgatcaa acgcggtttc ccacgcccag gttttccaca
     3121 cgcagttaac cagtcgccct tccccatcaa tcagttgccc ggcagcatcc cagcccagtt
     3181 catccagccc gcgcaagata cgcgtttcaa agcccgcctg gtgcagcgcc tgctccataa
     3241 actgcgcgtg atagttttcc tcgatatctt tgtcctgcat gatatggaca aacggacgtg
     3301 cacgactgtg tttccaggca ccagccagtt cgttaatcag cccttccgcc ggattgaagc
     3361 cgttgccttt atagccctgc tccgcccaac gttcgaggat caagcccgct tcggtatgac
     3421 atgaggcaga gtcggcgttg tactcgtaaa ccttcagacc acgctcatcc atgcagaaat
     3481 ccatacgacc ggtgatcata tggtgacggc gacgctgcca ggagagacgc aaacgtggcc
     3541 agaggatttt cgggatgtcg aacagcgcca gcaggttgtc atctttcagc accttgtcgg
     3601 ttgcgtgaag atacatcagg tgcagctcgt tggtggcttt aattagctcc tgctcggcac
     3661 tctcggtaat ggtgtagtaa tgataaggat cctgattgat cacctgaccg ttggcctgca
     3721 cataggcgtt ttgcagcgga tctttttcat ccagccattt accgtcaaac tggcctttgt
     3781 tttccaggcg cgctccgctg attttcagca gctcgcctgc aatttccggc tgcggtaagc
     3841 tgtattcagt atcttccgtc tggatcatcc agcccagaat ggtggtgtca tcaaaagtgt
     3901 ctttcagggt atagcagccg ttttcgacca ccatctccag ctcacgcgtc cactgttgcc
     3961 cttgcggcaa cggggaatga atcacgttct gttccgcaat acggactttg ttgccatgca
     4021 attgggtaat gatggcgaca tggccagtgt ctttaaattc accgccttta tcccagataa
     4081 gaagcgcacc cgcgaccggc gcacgcggcg agccgttagg aaatgcctgc aatggcagga
     4141 tgttgtcatt aaccacttct cgcaggaagc gcagcgagaa aatctcccac gccataccca
     4201 cgtcagtaaa gaccacaccg taattcagaa agagaaaacg gcgagcaaat tcaacgcatt
     4261 gccacttgtg gcccatatat tcgtcgtcga tatagctacg gaatacggcg tcatcttcgt
     4321 attcctgcgg atcgagagaa ctgtaatctg aagagtagat tgctacccca cctggggcgt
     4381 agcccaataa tgtcccgaac ggggcatcct ggctggtcgt tcctttgctc atcactttac
     4441 ctttaacaat acaacctaag cagttggcgt aattgttgtg ctctgattac ctgctcagcg
     4501 acattaaccg gacagaggtc agactaatca tacacctcat cgcagcggct ggcgcaaagg
     4561 gttaaaaaat tcacattctg tacagcaagt gacctgctac actgcttcaa cactaccact
     4621 cagaaggcaa ctcactatga cagacaatac ttatcagccc gcgaaagtct ggacgtggga
     4681 taaatccgct ggcggcgcgt tcgccaatat caatcgcccg gtttctggtc cgacgcatga
     4741 aaaaacgctg cccgttggca aacacccatt gcaactttat tcgctgggaa cgccgaacgg
     4801 tcagaaagta acgattatgc ttgaggagct gctggcgctg ggcgttactg gtgcagagta
     4861 cgacgcctgg ctgattcgta ttggcgatgg cgatcaattc tccagcggct ttgtcgaagt
     4921 gaacccaaac tcgaagatcc cggcgctgcg cgatcatacg cataatccgc cgatccgcgt
     4981 gtttgaatct ggttcgatcc tgctttatct ggcggagaaa tttggctact tcctgccgca
     5041 ggatttggca aagcgtactg aaacgatgaa ctggctgttc tggttacagg gcgcggcacc
     5101 gttcctcggc ggtggttttg gtcactttta ccattacgcg ccgatgaaaa ttgagtacgc
     5161 catcaaccgc tttaccatgg aagccaaacg tctgctcgac gtgctggata agcaactggc
     5221 gcagcataag tttattgcgg gcgatgagta caccattgcg gatatggcga tttggccgtg
     5281 gtttggcaat gtggtgttag gcggcgtgta tgatgccgca gagtttcttg atgcgggcag
     5341 ttataagcat gtacaacgct gggcgaaaga agtaggcgaa cgtccggcgg tgaaacgtgg
     5401 gcgtattgtt aaccgcacca acggaccgct gaatgagcag ttgcatgagc gccatgacgc
     5461 cagtgatttc gagacgaata cggaagataa gcgtcagggg taagggttgg tgttcgtcgc
     5521 agcaagccat ccaggccgga taaggcgttc acgccgcatc cggcaatcgt gcacaatgcc
     5581 tgatgcgacg ctg
//
LOCUS       DAZGCS010000201         5569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGCS010000201 DAZGCS010000000
VERSION     DAZGCS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.204"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1824)
                     /locus_tag="ACHK01_004816"
                     /pseudo
     CDS             complement(1..1824)
                     /locus_tag="ACHK01_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1770..1991)
                     /locus_tag="ACHK01_004817"
     CDS             complement(1770..1991)
                     /locus_tag="ACHK01_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099611.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(2036..3973)
                     /locus_tag="ACHK01_004818"
     CDS             complement(2036..3973)
                     /locus_tag="ACHK01_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8099612.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDHPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGDVVT
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVTANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            complement(4037..>5569)
                     /locus_tag="ACHK01_004819"
     CDS             complement(4037..>5569)
                     /locus_tag="ACHK01_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8099613.1"
                     /translation="IVDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFIL
                     IPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDD
                     LCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLRE
                     AIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPE
                     MVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGL
                     ALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETRE
                     WLGWGHAWAHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAEL
                     LEHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQ
                     PLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVF
                     FI"
BASE COUNT         1162 a         1825 c         1376 g         1206 t
ORIGIN      
        1 gtaccggaat attcgcaacc gcatcattca ccgccttctg cagaacatcc ggatcgtagt
       61 cacgaccgtc gcgcggaaca ggaatatggc tcactgcctc tttcaccatc tgctcaatca
      121 tcggacgcac atcatcgggg gtgatacttt taccgtccgc cggtaccggt attttcccga
      181 ccgcatcatt caccgcctgc tgcagtacat ccggatcata gtcacgacca tcacgcggaa
      241 cagggatatg gcttgctgcg tccttcacca tctgctcaag catcggacgc acatcatcac
      301 ccgtcacgca cttctgtaat accacagact gagaagccag tttttcttca aacgtttgtg
      361 cctgcgcggc tattttctcc tcaaatgtac gctgtaaatc cgccagcacc atggcgaatt
      421 cttcgcccag tgcacgaata atggacagtt cccgttccgt cattttcgca gtatccccct
      481 gaacatcgct ttcaccgcat catgctctgt ttcactgatt gccttattac cgtcagatgc
      541 gccatcaggc agttgtgctg agactgtttt cccggacgac gcgaacggat cctcacgggc
      601 atcacgacgg gacagcgcct ccagactgta gttctgctgc tgaagataca gtgcatcacc
      661 tcccgcaagg ggcggcaggt tctcacgttt acgggcctca ttgggcgtca gcagcgtatt
      721 tttcaccgac tcacccagcg ttttcatgcg ccgttcgctg tccattctca gcagcgtggt
      781 gacgtcaaac tccgtactct cgttttcccc cgtttccagc gcctcatcca gtaacagctc
      841 aatggactca atcagcgtct gcagacactg ggaataatac tgctgctcca gcgcctccac
      901 gttatcactg gagggaggtt gtcccacacc aatcttgtag gccgggacac ggaacaccga
      961 acagacaatt tcagccgtca ttttcagttg ttccaccgtc tgcgcatcca ccggtgaaaa
     1021 cgtcgtgggg ttatatttcg ccccgttgct cagaatcgcc gttttccccg cattttcgcc
     1081 ggtatacccg ctgtcccagt tgctcttcag ttttttcgca ttttcttccg taatactgcc
     1141 ggggatctca atcaccccgg acggcctgcc gccatttctg aaaaaagacg ttgaatttgc
     1201 ctgaatatga tgcccctgcg tggcagccag cccggcagca tacaccggcg gcagccccac
     1261 aagcggatga aaaaaacagt taaaccggtc gtggatcact tcccgggcag gcaccgtcac
     1321 cgcctccgtg atcccgcagt tccggtccgg cgtgatgcga tagaacacct cgccgtcatc
     1381 cgccaccaga ggttcaaccc ggctccagtc cagaatacgc agttctttga tctgcccacg
     1441 ggagttacgg attttcagca ccaccgtatt gccgtgacgc agtttggcgt tcagccacag
     1501 ttcaaaaaac tggatacgat tctgctgtgc attgggacga cgacagagac gggcaatatc
     1561 cccctgccgt ttttcacggc ggatcccctg tgtatcggtc tgcatcaggc gcagtcgcat
     1621 tttggcgata tcctgggata tcagcgaaat gcaagaaaac accgcatgaa aggagaggac
     1681 actttccgga tcggctttca ctccctgctg ccaggcaccg gcaaaaggct cagccaccgc
     1741 ctgaaacagg ctggtccagc ccacctcttt tacatcacgt cctgatttct ggttttttcg
     1801 gggtcgccgc aaaaggttcc acattcgcca tgctccgcat cacgtttctt tttctgacct
     1861 gccggacgtc gcgccgtgat gtactccgcc ttccccaggc gaaccagcac ctccgcacac
     1921 ggctgtgcca catcacggat atccccggcc cgggcatcat gcgtgccctg cagatactgg
     1981 atttttgcca tcagttactg cgggaagctc gcgcctcccg ccctcctcat cagactcagc
     2041 cgccggacgc agttccgtag ttcacaccgg tgatcaccgc caccgccgca gtacggcgac
     2101 gacgccagtt gatccagcgc tccgcacgga tggccacgct gccggtctgg aacatggaga
     2161 ccagctccac cggtgacggt gtgctgctgt cgctggtcgg ttcagactgc atctccagtg
     2221 acgcttcacg ggacatatcc accgccacac caccgtcatc agccagataa atatccggcg
     2281 cattcaccag taccagctgg tcacccacat actgggagac aatcaccggc agcccctgga
     2341 aggtcccgcc cagcagggtc atgtccggat attccttctg ccccagcgca tttttacgca
     2401 tggacagcgc cagggcattc gtgctggaca tcagccagac cgcaccggtg ggctgcagat
     2461 ttgccgtcac aaactggcca aacgcggcct cggcatccgc atccggatta ccgcttgacg
     2521 ccgtgccctt cacatcatgg gtgatggacg caggggagac atccgccacc gccgcttttt
     2581 tcggatcaac aaagtctgtg tccagacgtg ccaccaccgc ctctgccagc gcattacgga
     2641 ccagtgcatc agcagccggg ctggaaaaac ggatcagctc ttccgtcagt accgcaatgg
     2701 ccgacacctt cgcatgactg aaggtaatgg attcaaaatc aaacttcgtc agcggtctgg
     2761 ccttgccctc acccacccag cctgcagctc cgccggacac ctgggcatgc acacggatat
     2821 tgaacggaac ctgacgaagg gccgggatcc ccccctgacc aaatcgcccg ataatggtct
     2881 gcggacgcag gtaatcaata aagtcctgcg catattcctg gtattcagac aggctgcctg
     2941 cccactgtgg atctgtggtg gtccctgccc ccaccgccga tttcagaaca tgatgcagac
     3001 ggctgtcatc cggatactga cggcgggcca cttccagggc ttcggagcgg acacctttag
     3061 ccgcggccag cgatttggca aaacgtgcga aaccaatccc cttatccagt ttctgctcca
     3121 cacggatcac cggcgcagag gccaccgtca ccacgtcacc gttaccagcc tgtttcaccg
     3181 gctgtgccgt ggcggcctta ccggcttcca gttcacgcag gcgcttcagg tgagcatcca
     3241 cctgacggat ttccgccgcg gtgttgtcgt agtgctcttc ctcctccaca tccagcgtgc
     3301 gcccttcctc tgcggctttg gtcatgacct cctcaaggga ggctgccagc gctgcacgct
     3361 tgttttcaaa acttttaatc tgttcgccaa tattcattat ggtcttttcc ttatgaaaaa
     3421 cggttgttga ctgtgccgca gcgccggcag aagatgcgat tttcaccacc ggtttccggt
     3481 tgccggacgc ggcagaaaac gggtggtcgt aagatttaat ggtccggatg gtgcattccg
     3541 cattcgcggg cacggtgacg gcagacacct ccatcagttc ccagcgcaga aaatgcagtc
     3601 cgcctccgtc cagataagtg tattcatggg gacggaagcc cacggacagc cccctgacca
     3661 gcccggtctt aatggccgcc cagacctcat ccagccgggc agccagttgc gacggcatat
     3721 ccggtacggg cttcaccagc attgccgtga tttccagccc ttccctgacc cgacgcaccg
     3781 tacactggcc tacagggcgg gaatggtcat gctgccagag aaacgggatc gtactgccaa
     3841 actccgcccc ctccggctcc aggatgtcac catcccgatc cggagaaggc gttgacgcaa
     3901 tcccggtgat cacccgttca tcctcgctga aggatttcac cgtcagcagg gaacaggccc
     3961 gtttaagagt cacatcagcc tcctgaaaat aaaaaaaccg ccgcagcggt tcatgatggt
     4021 tacagggtga gcagggttat atgaaaaaaa cctcatacgc tttctttttc ggttccggat
     4081 tcagggacat cagggacacc gcattgaaga gcgccatcag cgggtcaatt tttccccgtc
     4141 cgctggcctg tttggtgata agaatggcgt tacctttagg ctccacccgg gcattgccaa
     4201 cgcaccaggc catcagtggc tgaccaccat gcaccagtac tccctcagcc agtttgcgct
     4261 cggtggtttt tatggccccg cccagcttcc agccctggct tatccccaca acaattccgt
     4321 cggggatccc ggcttccgcc agtgaatcca gaatctgccc cacacctgac gggtcaatac
     4381 cgatatgctc cagtaactca gcctcatgaa tacgacgcac atactccgcc acttccgccg
     4441 tgtcatcccc gacccgacgg acaatcgtca tgtctccaca ggccacaaaa tcctgaaaac
     4501 gggatgcctc actcttccgt ctgaccaccg cggtttcatg cgcccaggca tggccccagc
     4561 ccagccattc gcgggtttcc ctgtcacggc caatcacgta cattcccagc agatcatcca
     4621 ggcccccgcc gtcaatcccc accgtcacca catcagcgcg ctgcaggata tcgtccaggc
     4681 tgacgcgcct gccctgctgc tcccagaaat ccgcacccgc ccagcggtca gaacgcaggg
     4741 caagaccaat ttccacattg gcatgttttg acatgaagcc acgaaatgct tcctcaccag
     4801 cctcccgggc tttacggtac tcccggtaca gaaaagcctc atccaccgaa taaccgagat
     4861 tcggattgac catggcgagg ttttccatca gcaggtgagc cccgctttcc accatttcag
     4921 gagggtgttc aaatatcacc ggcagaaagt gcggatcatg aattttgccg tcacggacat
     4981 cccgggcgta ctgcagtttc tgtctgaaca ccccggcagg cggttcattc gactgggtgg
     5041 ttgtgtacac cacaaatcct tccggacggg aggcaagccc gccgatggct tcacgtagca
     5101 tgtcttccgc tttgtactgc ttgccaaaca gccacagttc atcaatcagt gtccccacgg
     5161 acttgatacc ggacaccgta ttcggatcgg ctgccaccac cttcagggtg gtgtccgtca
     5221 cccgatgggt gatggtccgg atatgtgtct gcacctgaca gaggtcatcc agatcatcgt
     5281 cccgtcgtac catatccctg gcagggttga aggcgttagc cgccacctcc acggtcgggg
     5341 ccagaatggt gtagcccgcc gcctgccgcc agttcagtaa cagcgccgtc atcatgatcc
     5401 ccgcagccag cgtggactta ctgtttttct tggggataag gataaacact tccttgatat
     5461 ggcgaacacc ggtctgcgca tcgtaggagc caaacagggc cgccaccagg tcaaacaccc
     5521 acggtgcgca ggactccccg aacgtcgggc tacccggtgc atccacaat
//
LOCUS       DAZGCS010000202         5458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGCS010000202 DAZGCS010000000
VERSION     DAZGCS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.205"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            102..1436
                     /gene="garP"
                     /locus_tag="ACHK01_004820"
     CDS             102..1436
                     /gene="garP"
                     /locus_tag="ACHK01_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO8099614.1"
                     /translation="MILDTVDEKKKDVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAHLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1452..2222
                     /gene="garL"
                     /locus_tag="ACHK01_004821"
     CDS             1452..2222
                     /gene="garL"
                     /locus_tag="ACHK01_004821"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO8099615.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2252..3142
                     /gene="garR"
                     /locus_tag="ACHK01_004822"
     CDS             2252..3142
                     /gene="garR"
                     /locus_tag="ACHK01_004822"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8099616.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3239..4384
                     /gene="garK"
                     /locus_tag="ACHK01_004823"
     CDS             3239..4384
                     /gene="garK"
                     /locus_tag="ACHK01_004823"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO8099617.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4417..4793
                     /gene="rnpB"
                     /locus_tag="ACHK01_004824"
     ncRNA           4417..4793
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK01_004824"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5301..>5458)
                     /locus_tag="ACHK01_004825"
     CDS             complement(5301..>5458)
                     /locus_tag="ACHK01_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099618.1"
                     /translation="LLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYN
                     YDLLNAI"
BASE COUNT         1300 a         1294 c         1531 g         1333 t
ORIGIN      
        1 actaattccc gagttcattg ccgttacggc ataaatgaaa ctcataaata acaccttccc
       61 cataataata aaaatcagca taagtacccg aggtaaataa aatgattctg gacaccgttg
      121 acgaaaaaaa gaaagacgtg catacccgct atttaatatt actgattatt tttattgtta
      181 ccgccgttaa ctacgccgat cgagcaacgc tgtctattgc tggtaccgaa gtggcaaaag
      241 agttgcagtt aagtgcggtt tcgatgggtt acatcttttc tgcttttggc tgggcccact
      301 tgctgatgca aatccccggc ggctggctgc ttgataagtt tggctcgaaa aaagtttata
      361 cctatagcct ctttttctgg tcgctattca ctttcctgca aggctttgtt gatatgttcc
      421 cgctggcctg ggcagggatc tccatgttct ttatgcgctt tatgctcggc ttctcggaag
      481 cgccatcatt cccggcgaac gcccgtattg tcgccgcctg gttcccgacg aaagaacgtg
      541 gtactgcctc cgccatcttt aactcggcgc aatatttctc gctggcgctc ttttcgcctc
      601 tgcttggctg gctgactttc gcctggggct gggagcacgt ctttaccgtt atgggggtga
      661 ttggttttgt gctgacggcg ctgtggatca aattgattca taacccgaca gatcacccac
      721 gtatgtctgc ggaagagctg aagtttatct ctgaaaatgg cgcggtggtc gatatggacc
      781 acaaaaagcc gggcagtgcg gcagcaagcg gtcccaaact gcattacatc aagcaattgc
      841 tctctaaccg catgatgttg ggcgtatttt tcggacaata ttttatcaac accatcacct
      901 ggttcttcct cacctggttc ccgatttatc tggtgcagga aaaaggcatg tcgattctga
      961 aagtgggtct ggtcgcctcg attccggcac tgtgtggttt tgcgggcggc gtgctgggag
     1021 gtgtcttctc ggattatctg atcaaacgcg gtttatccct gaccctggca cgtaagctac
     1081 cgattgtgct gggaatgttg ctggcttcca ccatcatctt atgtaactac accaacaaca
     1141 ccacgctggt ggtcatgcta atggcgctgg ctttttttgg caaaggattt ggtgctctgg
     1201 gctggccggt gatttctgac accgcgccga aagagattgt tggcctctgt ggcggcgtct
     1261 ttaacgtctt tggcaatgtt gcctccattg tcactccact ggtgattggc tacctggtaa
     1321 gtgaactgca ctccttcaat gcagcactgg ttttcgtggg atgttcagcg ctgatggcga
     1381 tggtctgcta cctcttcgta gttggcgaca ttaaacgtat ggaattgcag aaataagcaa
     1441 aggtataagc gatgaataac gatgttttcc cgaataaatt caaagccgca ctggctgcga
     1501 aacaggtaca aattggttgc tggtcagcac tctctaaccc gattagcact gaagttcttg
     1561 gtttggctgg gtttgactgg ctggtgctgg atggcgaaca tgcgccaaac gatatctcca
     1621 cgtttattcc gcagttaatg gccctgaaag gcagcgccag cgcgccagta gtgcgagtgc
     1681 cgaccaacga gccggtaatt attaagcgtc ttctggatat cggtttctat aacttcctga
     1741 ttccttttgt agaaacaaaa gaggaagcag agcaggcggt ggcatcaacc cgttacccac
     1801 cggaaggcat tcgcggcgtc tccgtttctc accgcgccaa tatgtttggc accgtggcgg
     1861 attatttcgc tcagtcgaac aagaacatca ccattctggt ccagatagaa agtcagcagg
     1921 gcgtagataa cgtcgatgcc attgccgcta ccgaaggtgt agacggtatc ttcgtcggcc
     1981 ccagcgatct ggctgcggca ttaggccatc tcggcaatgc ctcgcacccg gatgtacaaa
     2041 aagcaattca gcacattttt aaccgtgcca gtgcgcacgg caaacccagc ggtattctcg
     2101 cgccggtcga agccgatgcg cgtcgttatc tggaatgggg cgcgacgttt gtggctgtcg
     2161 gcagcgatct tggcgtcttc cgctctgcca ctcagaaact ggctgatacc tttaaaaaat
     2221 aaccaccacc gcaacaagag agatgattga tatgactatg aaagttggtt ttattggcct
     2281 ggggattatg ggtaaaccaa tgagtaaaaa ccttctgaaa gcaggttact cgctggtggt
     2341 tgctgaccgt aacccagaag ctattgctga cgtgattgct gcaggtgcag aaacagcgtc
     2401 tacggctaaa gcgatcgctg aacagtgcga cgtcatcata accatgctgc caaactcccc
     2461 tcatgtgaaa gaggtggcgc tgggtgagaa tggcatcatt gaaggcgcga agccaggtac
     2521 ggtattgatc gatatgagtt ctatcgcacc gctggcaagc cgtgaaatca gcgaagcgct
     2581 gaaagcgaaa ggcattgata tgctggatgc tccggtgagc ggcggtgaac cgaaagccat
     2641 cgacggtacg ctgtcagtga tggtgggcgg cgacaaggct attttcgaca aatactatga
     2701 tttgatgaaa gcgatggcgg gttccgtggt gcataccggg gaaatcggtg caggtaacgt
     2761 caccaaactg gcaaatcagg tcattgtggc gctgaatatt gccgcgatgt cagaagcgct
     2821 aacgctggca accaaagcgg gcgttaaccc ggacctggtg tatcaggcaa ttcgcggtgg
     2881 actggcgggc agtaccgtgc tggatgccaa agcgccgatg gtgatggacc gcaacttcaa
     2941 gccgggcttc cgtattgatc tgcatattaa ggatctggca aatgcgctgg atacttccca
     3001 cggcgtcggc gcgcaactgc cgctcacagc tgcggttatg gagatgatgc aggcactgcg
     3061 agcagatggt ttaggaacgg cggatcatag cgccctggcg tgctactacg aaaaactggc
     3121 gaaagtcgaa gttactcgtt aatgacgtcg tgcccggatg gcgtattgca atccgggcct
     3181 ggaatccagg ccgaataaga gaaacgccct ccggcgtcat gtggtaacag gcataggtat
     3241 gaaaatcgta atcgccccag actcttataa agaaagttta tctgccagcg aggttgcgca
     3301 ggcgatagaa aaaggatttc gggaaatttt tcctgatgca cagtacgttt ctattccggt
     3361 tgccgacggt ggcgaaggaa cggtggaagc gatgattgca gccacccagg gttctgaacg
     3421 tcacgcctgg gttacagggc cgctgggcga gaaagtgaat gccagttggg ggatctccgg
     3481 cgatggcaaa accgcgttta ttgaaatggc ggcggccagt gggctggagc tggtacctgc
     3541 ggaaaaacgc gatccactcg tgaccacttc acgcggcaca ggcgagttaa tcctgcaggc
     3601 gctggagagc ggcgcgacaa acattattat cggcattggc ggtagcgcta caaatgatgg
     3661 cggcgcaggc atggtacagg cgctgggggc gaaattatgc gacgccaacg gcaatgaaat
     3721 tggttttggc ggcggtagtc ttaatactct gaatgatatt gatatttccg gcctcgatcc
     3781 gcgcttaaaa gattgcgtca ttcgcgtcgc ttgtgatgtc accaatccgc tggtgggcga
     3841 taacggcgca tcgcgcatct ttggcccaca aaagggagcc agtgaagcga tgattgttga
     3901 gctggacaat aacctctctc actatgccga ggtcattaaa aaagcgctgc atgttgatgt
     3961 gaaagatgtc cccggcgcag gagctgcggg cggtatgggt gcggcgctga tggcgtttct
     4021 tggtgcggaa ctgaaaagtg gtattgaaat cgtcactacg gcgctgaatc tggaggaaca
     4081 tattcacgat tgtacgctgg tgatcaccgg tgaagggcgt attgacagcc agagtattca
     4141 cggcaaggta ccgattggcg ttgcaaacgt ggcgaagaag taccataaac cggtgattgg
     4201 cattgcgggt agcctgaccg atgatgttgg cgttgtacat cagcatggca tcgatgcggt
     4261 cttcagcgta ttgaccagca taggtacgtt ggacgaagca ttccgcgggg cttatgacaa
     4321 tatctgccgt gcttcacgta atatcgccgc gacactggcg attggaatgc gcaacgcggg
     4381 gtgacaaggg cgcgcaaacc ctctatactg cgcgccgaag ctgaccagac agtcgccgct
     4441 tcgtcgtcgt cctcttcggg ggagacgggc ggaggggagg aaagtccggg ctccataggg
     4501 cagggtgcca ggtaacgcct gggggggaaa cccacgacca gtgcaacaga gagcaaaccg
     4561 ccgatggccc gcgcaagcgg gatcaggtaa gggtgaaagg gtgcggtaag agcgcaccgc
     4621 gcggctggta acagtccgtg gcacggtaaa ctccacccgg agcaaggcca aataggggtt
     4681 cataaggtac ggcccgtact gaacccgggt aggctgcttg agccagtgag cgattgctgg
     4741 cctagatgaa tgactgtcca cgacagaacc cggcttatcg gtcagtttca cctgatttac
     4801 gtaaaaaccc gcttcggcgg gtttttgctt ttggaggggc agaaagatga atgactgtcc
     4861 acgacactat acccaaaaga aagcggctta tcggtcagtt tcacctgatt taacgaaaac
     4921 ccgccagcgt aaaccggcgg gtttttgctt ttggaggggc agaaagatga atgactgtcc
     4981 acgacgctat acccaaaaga aagcggctta tcggtcagtt tcacctgatg tacgtaataa
     5041 accgttccgg cgggtttcag attgttgagt gcgctttatt catgccggat gcggcgtgaa
     5101 cgccttatcc tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg gcgtaggcct
     5161 gataagcgta gcgcatcagg catttttgcg gttgccatca gtttcaaacc cgcgtcagtg
     5221 agtttttgct tttggtgcac agggtaagat gaataaatat ctacggtatt aataatagac
     5281 tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaattata cgtatagtcc
     5341 tctctggtaa aatattgatt ctctgagtta tatgcttcat atgtgttata ttccccgacc
     5401 tcaaaaccat tgtccggaga attttcgtct ttctcgaggg tgttatctga aagtaact
//
LOCUS       DAZGCS010000203         5374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGCS010000203 DAZGCS010000000
VERSION     DAZGCS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.206"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..327
                     /locus_tag="ACHK01_004826"
     CDS             <1..327
                     /locus_tag="ACHK01_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO8099619.1"
                     /translation="ETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAFCTFV
                     LSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSE
                     YIEQSG"
     gene            302..2227
                     /locus_tag="ACHK01_004827"
     CDS             302..2227
                     /locus_tag="ACHK01_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8099620.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLAEYARALSGEDE"
     gene            2224..2430
                     /locus_tag="ACHK01_004828"
     CDS             2224..2430
                     /locus_tag="ACHK01_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO8099621.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2427..4028
                     /locus_tag="ACHK01_004829"
     CDS             2427..4028
                     /locus_tag="ACHK01_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8099622.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4009..5328
                     /locus_tag="ACHK01_004830"
     CDS             4009..5328
                     /locus_tag="ACHK01_004830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8099623.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPTALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
BASE COUNT         1231 a         1414 c         1658 g         1071 t
ORIGIN      
        1 gagacagatc tccagccagg gactattgag tacgaacgcc atcgacttac gcgtgcgcag
       61 gccgacgcac aggagctgaa aaatgccaga gactccgctg aagtggtgga aaccgcattc
      121 tgtactttcg tgctgtcgcg gatcgcaggt gaaattgcca gtattctcga cgggatcccc
      181 ctgtcggtgc agcggcgttt tccggaactg gaaaaccgac atgttgattt cctgaaacgg
      241 gatatcatca aagccatgaa caaagcagcc gcgctggatg aactgatacc ggggttgctg
      301 agtgaatata tcgaacagtc aggttaacag gctgcggcat tttgtccgcg ccgggcttcg
      361 ctcactgttc aggccggagc cacagaccgc cgttgaatgg gcggatgcca gttactatct
      421 cccgaaagaa tccgcatacc aggaagggcg ctgggaaaca ctgccctttc agcgggccat
      481 catgaatgcg atgggcagcg actacatccg cgaggtgaat gtggtgaagt ctgcccgtgt
      541 tggttattcc aaaatgctgc tgggtgttta tgcctacttc atagagcata agcagcgcaa
      601 cacacttatc tggttgccga cggatggtga tgccgagaac tttatgaaaa cccacgttga
      661 gccgaccatc cgcgatattc cgtcgctgct ggcgctggct ccgtggtatg gcaaaaagca
      721 ccgggataac acgctcacta tgaagcgttt ttccaatggt cgtggcttct ggtgcctggg
      781 cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat gtggcgggtt atgatgaact
      841 tgctgccttt gatgaggata ttgaacagga aggctctccg acgttcctgg gcgataagcg
      901 tattgaaggc tcggtctggc caaagtccat ccgtggctcc acgcccaaag tgagaggcac
      961 ctgccagatt gagcgtgcag ccagtgaatc cccgcatttt atgcgttttc atgttgcctg
     1021 cccgcactgc ggggaggagc agtatcttaa atttggcgac aaagagacgc cgtttggcct
     1081 caaatggacg ccggatgacc cctccagcgt gttttatctc tgcgagcata atgcctgcgt
     1141 catccgccag caggagctgg actttactga tgcccgttat atctgcgaaa agaccgggat
     1201 ctggacccgt gatggcattc tctggttttc gtcatccggt gaagagattg aaccgcctga
     1261 cagtgtgacc tttcacatct ggacggcgta cagcccgttc accacctggg tgcagattgt
     1321 caaagactgg atgaagacga aaggggatac gggaaaacgt aaaaccttcg tgaacaccac
     1381 gctcggtgag acgtgggaag cgaaaatcgg cgaacgtccg gatgctgagg tgatggcgga
     1441 gcggaaagag tattattcag cgcccgttcc tgatcgtgtg gcttacctga ccgccggtat
     1501 cgactcccag ctggaccgct acgaaatgcg cgtatgggga tgggggccgg gtgaggaaag
     1561 ctggctgatt gaccggcaga ttattatggg ccgccacgac gatgaacaga cgctgctgcg
     1621 tgtggatgag gccatcaata aaacctatac ccgccggaat ggtgcagaaa tgtcggtatc
     1681 ccgtatctgc tgggatactg gcgggattga cccgaccatt gtgtatgaac gctcgaaaaa
     1741 acatgggctg ttccgggtga tccccattaa aggggcatcc gtctacggaa agccagtggc
     1801 cagcatgcca cgtaagcgaa acaaaaacgg ggtttacctt accgaaatcg gtacggatac
     1861 cgcgaaagag cagatttata accgcttcac actgacgccg gaaggggatg aaccgcttcc
     1921 cggtgccgtt cacttcccga ataacccgga tatttttgat cttaccgaag cgcagcaact
     1981 gactgctgaa gagcaggtcg aaaaatgggt ggatggcagg aaaaaaatac tgtgggacag
     2041 caaaaagcga cgcaatgagg cgctcgactg cttcgtttat gcgctggcgg cgctgcgcat
     2101 cagtatttcc cgctggcagc tggatctcag tgcactgctg gcgagcctgc aggaagagga
     2161 tggtgcagca accaacaaga aaacactggc agaatacgcc cgtgccttat ccggagagga
     2221 tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg cactgcatga cctgatgaca
     2281 ggaaaacggg tggcaacggt acagaaagac ggacggcgag tggagtttac gaccacttcc
     2341 gtgtctgacc tgaaaaaata cattgctgag ctggaagtgc agaccggcat gacacagcga
     2401 cgcaggggac cagcaggatt ttatgtatga aaatgtccac cattcccacc cttctggggc
     2461 cggacggcat gacatcgctg cgtgaatatg ccggttatca cggcggtggc agcggatttg
     2521 gtgggcagtt gcgggcgtgg aacccaccgg gtgaaagtgt ggatgcagcc ctgctgccca
     2581 actttacccg tggcaatgcc cgcgcagacg atctggtacg caataacggc tatgccgcca
     2641 acgccatcca gttgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc
     2701 caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg
     2761 aagcggcatg gaaagagttt gccgaagatg actgttgctg cattgacgtt gagcgaaaac
     2821 gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt
     2881 tcgttcaggc cacctgggat acccgtccct cgcgactgtt ccggacacag ttccggatgg
     2941 tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg
     3001 gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc
     3061 ctggctggat gccgcagaaa tggacatgga taccccgcga gttacccggc ggtcgtgctt
     3121 cgttcattca cgtctttgaa cccgtggagg acgggcagac ccgcggtgca aatgtgtttt
     3181 acagcgtgat ggagcagatg aagatgctcg acacgctgca gaacacgcag ctgcagagcg
     3241 ccattgtgaa ggcgatgtat gccgccacca ttgagagtga gctggatacg cagtcagcga
     3301 tggattttat tctgggcgcg aacagtcagg agcagcggga aaggctgacc ggctggattg
     3361 gtgaaattgc cgcgtattac tccgcagcac cggtccgtct gggaggcgca aaagtgccgc
     3421 acctgatgcc gggggactca ctgaacctgc agacggctca ggacacggat aacggctact
     3481 ccgtgtttga gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc
     3541 agctttcccg gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt
     3601 cgtgggcgta ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt
     3661 ttctgtgctg gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc
     3721 gcttcagctt tcaggaagcc cgcagtgcct gggggaactg cgactggata ggctccggtc
     3781 gtatggccat cgatggtctg aaagaagttc aggaagcggt gatgctgata gaagccggac
     3841 tgagcaccta cgagaaagag tgcgcgaaac gcggtgacga ctatcaggaa atttttgccc
     3901 agcaggtccg tgaaacgatg gagcgccgcg cagctggtct taaaccgccc gcctgggcgg
     3961 ctgcggcatt tgaatccggg ctgcgacaat caacagagga ggagaagagt gacagcagag
     4021 ctgcgtaatc tcccgcatat tgccagcatg gcttttaatg agccgctgat gcttgaaccc
     4081 gcctatgcgc gggttttctt ttgtgcgctt gcaggccagc ttgggatcag ccgcctgacg
     4141 gatgcggtgt ccggcgacag cctgactgcc ggagaggcac ccacggcgct ggcgttatcc
     4201 ggtgatgatg acggaccacg acaggcccgc agttatcagg tcatgaacgg catcgccgtg
     4261 ctgccggtgt ccggtacgct ggtcagccgg acgcgggcgc tgcagccgta ttcgggaatg
     4321 accggttaca acggcattat cgcccgtctg caacaggctg ccagcgatcc gatggtggac
     4381 ggcattctgc tggatatgga cacaccgggc gggatggtgg cgggagcatt tgactgtgct
     4441 gacatcatcg cccgtgtgcg agacataaaa ccggtatggg cgctggccaa cgacatgaac
     4501 tgcagtgcag gtcagctgct tgccagcgcc gcctcccggc gtctggtcac gcagaccgcc
     4561 cggacaggct ccatcggcgt catgatggct cacagtaatt acggtgctgc gctggagaaa
     4621 cagggcgtgg aaatcacgct gatttacagc ggcagccata aggtggatgg caacccctac
     4681 agccatctac cgggtgatgt ccgggaaaca ctgcagtccc ggatggatgc aacccgccgg
     4741 atgtttgcgc agaaggtgtc ggcatatacc ggcctgtccg tgcaggctgt gctggatacc
     4801 gaggctgcag tgtacagcgg tcaggaggcc attgatgccg gactggctga tgaacttgtc
     4861 aacagcaccg atgcgatcac cgttatgcgt gatgcactgg atgcacgtaa atcccgtctc
     4921 tcaggagggc gaatgaccaa agagactcaa tcaacaactg tttcagccac tgcttcgcag
     4981 gctgacgtta ctgacgtggt gccagcgacg gagggcgaaa acgccagcgc ggcgcagccg
     5041 gacgtgaacg cgcagatcac cgcagcggtt gcggcagaaa acagccgcat tatggggatc
     5101 ctcaactgtg aggaggctca cggacgcgaa gaacaggcac gcgtgctggc agaaaccccc
     5161 ggtatgaccg tggaaacggc ccgccgcatt ctggccgcag caccacagag tgcacaggcg
     5221 cgcagtgaca ctgcgctgga tcgtctgatg cagggggcac cggcaccgct ggctgcaggt
     5281 aacccggcat ctgatgccgt taacgatttg ctgaacacac cagtgtaagg gatgtttatg
     5341 acgagcaaag aaacctttac ccattaccag ccgc
//
LOCUS       DAZGCS010000204         5353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGCS010000204 DAZGCS010000000
VERSION     DAZGCS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.207"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            340..858
                     /locus_tag="ACHK01_004831"
     CDS             340..858
                     /locus_tag="ACHK01_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8099624.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            891..1028
                     /locus_tag="ACHK01_004832"
     CDS             891..1028
                     /locus_tag="ACHK01_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099625.1"
                     /translation="MLFLYYAVERKVVSKGLRSREMAAAGNFGGSVPERKRNTVLSQP
                     L"
     gene            1068..3209
                     /locus_tag="ACHK01_004833"
     CDS             1068..3209
                     /locus_tag="ACHK01_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8099626.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3285..>5353
                     /locus_tag="ACHK01_004834"
     CDS             3285..>5353
                     /locus_tag="ACHK01_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8099627.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRY"
BASE COUNT         1255 a         1369 c         1682 g         1047 t
ORIGIN      
        1 gtatttttaa ggaatatctc aaaaattatc aaaatgataa ttcagaaagg ataaatacaa
       61 tatttattca tgattcagct ttggccattt tatggaggca atacggactt tgaacagttg
      121 ggtatggtta tccagatata tattattaag ttattgctga ataagagatt taatgatttt
      181 aaaaatagtt tttacgacaa ccgtaaattc tagctcggtt tgtatgtaaa aaatactgaa
      241 aaatgatgcg gttccgtgaa gtgagctgca tctgcctgtc tgggattaac ggcaggtcat
      301 tgtaagtagt aattaaccac gttaacaagg agtttacaaa tgccaacacc gtgttatatc
      361 tccatcactg gtcagaccca gggaaatatt actgcaggtg catttaccgc tgattctgtc
      421 ggcaacatct atgtgcaggg gcacgaagac gagatgctgg tgcaggaatt cctgcataac
      481 gttactgtcc cgaccgatcc tcagtccggt cagcctgcgg gtcagcgtgc tcacaagcct
      541 ttcattttta ccgtggcgct gaacaaagcg gttccgctga tgtacaacgc actggcctcc
      601 ggcgaaatgc tgccaaccac tgaacttcac tggtggcgta cctctgtgga aggcaaacag
      661 gagcattatt tcactacccg cctgactgat tcgacgattg tcgatatgaa actgcatatg
      721 ccgcattgcc aggacccggc gaaacgcgag ttcacccagt tgctcgaagt ttcactggcc
      781 taccgcaaaa ttgaatggga acacgtcaaa tctggcacct ccggtgccga cgactggcgc
      841 gcaccgctgg aagcataagt taagccaaca gcatcctgct atcaggccgg atgctgtttt
      901 tgtattacgc cgtagaaaga aaggttgtca gtaaggggtt acgttcacgg gagatggcgg
      961 ctgccggaaa cttcggcggt agcgtgcctg aacgcaagcg taacactgta ctgtcacagc
     1021 cgctttaaca gcgaactgtc tgtttttcct taaccccgga ggttgctatg tcaaccggat
     1081 tacgtttcac gctggaagtg gacggcctgc caccggatgc ttttgcggtg gtctcctttc
     1141 atctgaacca gtcactctct tcgctttttt ccctcgatct ctctctggtc agccagcagt
     1201 ttctctccct tgaattccag cagatcctcg acaaaatggc ctacctgacg atatggcagg
     1261 gcgatgacgt acagcgccgg gtgaaaggta tggtgacctg gtttgaactg ggggagaacg
     1321 acaaaaacca gatgctgtac agcatgaagg tgtgcccacc gctgtggcgc acagggctgc
     1381 gccagaactt ccgtatcttc cagaatgagg acatcgaaag catcctcggc acgatattgc
     1441 aggaaaacgg ggtgaccgag tggagcccgc tgttcagcga gccacatcct tcccgtgagt
     1501 tttgtgtcca gtacggtgag actgattacg atttcctgtg ccggatggcg gcggaggaag
     1561 gcatcttctt ttatgaggag cacgcgcaaa aaagtaccga ccagagcctg gtcctgtgcg
     1621 ataccgtgcg ttatctgccg gagtcctttg agatcccctg gaatccgaac acccgtaccg
     1681 aggtgagcac cctctgcatc agccagtttc gctacagcgc acaaatccgc ccttcttccg
     1741 tggtgaccaa agactacacc tttaaacgac ccggctgggc agggcgtttt gatcaggaag
     1801 gccagtacca ggattaccag cgcacacagt atgaagtgta tgactacccc ggacgtttca
     1861 agggtgccca cgggcagaac tttgcccgct ggcagatgga tggctggcgc aacaacgcag
     1921 aagtggcgcg cggaacaagc cgttcgccgg agatatggcc gggacggcga attgtgctga
     1981 cggggcatcc gcaggcgaac ctgaaccggg aatggcaggt ggtggcgagc gatcttcacg
     2041 gcgaacagcc gcaggcggta ccgggacgca ggggttcagg caccacgctg gataaccact
     2101 ttgcggtaat cccggcagac cggacatggc ggccacagcc gttgctgaaa ccgctggtgg
     2161 acggcccgca gagcgccgtc gtgacgggac cggcaggcga ggaaatcttc tgtgatgaac
     2221 atggccgcgt gcgggtgaaa tttaactggg accgctataa cccgtcaaac caggaaagtt
     2281 catgctggat ccgtgtggca caggcgtggg caggcaccgg ctttggtaac ctggcgatac
     2341 cgcgtgtggg tcaggaggtc attgtggact tcctcaacgg cgatccggac cagccgatca
     2401 ttatggggcg tacctaccac caggaaaacc gcacccccgg cagcctgccg gggacgaaga
     2461 cgcagatgac gatccgctcg aaaacctata agggcagcgg gtttaatgaa ctgaagtttg
     2521 acgatgcgac agggaaagaa caggtctaca tccacgcgca gaagaacatg aacaccgagg
     2581 tgctgaataa ccgcaccact gatgtgataa acaaccatgc cgaaaaaata ggtaacaacc
     2641 aggcgatcac cgttaccaat aaccagatcc agaacattgg cgttaatcag atacagacgg
     2701 ttggtgtcaa ccaggtggaa acggtgggca gtaaccagat tatcaaagtg ggatcaaacc
     2761 aggttgaaaa ggtggggatc attcgtgcgc tgacggtggg tgtggcttac cagacgacgg
     2821 taggcggcat tatgaatacc tcggtggcgt tgttgcagtc ctcacaggta gggctgcata
     2881 aatcactgat ggtgggaatg ggctacagcg tcaatgtggg gaataacgtc accttctcgg
     2941 tgggcaagac aatgaaggaa aacaccggac aaacagcagt ttattctgcc ggtgagcatc
     3001 ttgaactctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggaagc atatttctca
     3061 atggtacgca cattcatctg gaaggggagt cggatgtgaa cggtgatgcg ccagtgatta
     3121 actggaactg tggtgccaca caacctgtac cggatgcgcc tgtgccgaaa gatttacccc
     3181 ccggaatgcc ggatatgcgg caattttgag tcaggtgcaa aaaattaaaa cggaactggt
     3241 gcagtgacca gataaaccat attaaaagca gaaggaattg attcatgggc ggaaaaccgg
     3301 cggcgcggca gggtgatatg acccgcaagg gactggatat tgtgcagggt tcagcggggg
     3361 tgctgatagg tgcgccgacg ggagtggcct gttcggtatg tcccaacagc ccgatagagg
     3421 agcagaaggg taacccggtg aacccgctgc tgggagcaaa ggtgctgccg ggcgagacgg
     3481 accttgcgct gcccggcccg ctgccgttta ttctttcccg cgcctacagc agctaccgga
     3541 ccagaacacc cgcgccggtg ggggtgtttg gtcccggctg gaaagcgccc ttcgatatcc
     3601 gcttacagat acgcgatgaa ggcctgatac tcaacgacag cggcggcagg agcattcact
     3661 ttgagccgct gtttcccggc gagataagct acagccgcag cgagtcactc tggctggcgc
     3721 ggggcggagt agcggcgcag cacagttcgc agccgctaag cgcgctctgg caggtgctgc
     3781 cggaagatgt tcgcctgagt ccgcatgtgt acctggcgac aaacagcctt caggggccgt
     3841 ggtggatact cagctggccg gagcgggtgc cgggggcgga cgaggtgctg ccgccggagc
     3901 cgcccgcata ccgggtgctg acgggcgtgg tggacggctt cgggcggacg ctggcctttc
     3961 accgggcggc ggagggtgat gtggcgggcg cggtgacggg ggtgacggac ggcgcgggcc
     4021 gccgttttca cctggtgctg accacacagg cacagcgggc ggaagtattc cgtaaacagc
     4081 gcgccacgtc tttatcttcc cctgccggtc cccgttctgc ttcctcttct ctggttttcc
     4141 ccgacacgct gcccgccggt acaggatacg gcacggataa cggtatccgg ctggaggcgg
     4201 tatggctgac acacgacccg gcatacccgg atgaacagcc gactgcgccg ctggcgcgct
     4261 acacgtacac ggccggtggt gaactgcggg cggtgtatga ccgcagcgga acgcaggtgc
     4321 gcgggtttac ttatgatgcg gagcacgccg ggcggatggt ggcgcaccat tatgcgggga
     4381 ggccggagag ctgctaccgg tatgatgata ccggtcgggt gacggagcag gtcaacccgg
     4441 aggggctgga ctaccgcttt gagtacggag agagccgggt aatcatcacg gacagcctga
     4501 accggcggga ggtgctgtac acggaaggcg agggtggcct gaagcgggtg gtgaagaagg
     4561 aacatgcgga cgggagcatt acccgcagcg agtatgatga ggcggggagg ctgaaggcac
     4621 agacggatgc ggcggggcgg cggacggagt acagcctgca tatggcgtcg ggtgcggtga
     4681 cggcggtgac ggggccggac ggcaggacgg tgcggtatgg ctataacagc cagcggcagg
     4741 tgacgtcagt gacgtacccg gacgggcttc gcagcagccg ggagtatgat gagaggggaa
     4801 ggctgacggc ggagacctcg cgcagcggtg agacaacgcg gtacagctat gatgacccgg
     4861 cgagtgagct gccgacaggg atacaggacg cgacgggcag tacaaaacag atggcatgga
     4921 gccgttacgg tcaactgctg gccttcacgg actgctcggg gtacacgacg cggtatgagt
     4981 acgaccggta cggtcaacaa atcgccgttc accgggagga aggcatcagc acttacagca
     5041 gttataaccc gcgtgggcaa ctggtcagtc agaaggatgc gcaggggcgt gaaatccgtt
     5101 atgagtacag cgccgcaggc gacctgacag caacggtatc accggacggt aaacgcagca
     5161 ccatcgaata tgataaacgc gggcgtccgg tgagtgtgac ggaaggcggt ctgacgcgca
     5221 gcatggggta tgacgctgcc gggcgcatca ctgtgctgac caacgagaac ggcagccagt
     5281 ccacgttccg gtatgacccg gtcgacaggc tgactgaaca gcgcggtttt gacggccgga
     5341 cgcaacgtta cca
//
LOCUS       DAZGCS010000205         5260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGCS010000205 DAZGCS010000000
VERSION     DAZGCS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.208"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..862
                     /locus_tag="ACHK01_004835"
     CDS             164..862
                     /locus_tag="ACHK01_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8099628.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(907..1806)
                     /gene="eamA"
                     /locus_tag="ACHK01_004836"
     CDS             complement(907..1806)
                     /gene="eamA"
                     /locus_tag="ACHK01_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO8099629.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGACTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2001..3188
                     /gene="ydeE"
                     /locus_tag="ACHK01_004837"
     CDS             2001..3188
                     /gene="ydeE"
                     /locus_tag="ACHK01_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO8099630.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3315..3410
                     /gene="mgtS"
                     /locus_tag="ACHK01_004838"
     CDS             3315..3410
                     /gene="mgtS"
                     /locus_tag="ACHK01_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO8099631.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3410..3514
                     /gene="mgtT"
                     /locus_tag="ACHK01_004839"
     CDS             3410..3514
                     /gene="mgtT"
                     /locus_tag="ACHK01_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO8099632.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3629..4519)
                     /gene="dgcZ"
                     /locus_tag="ACHK01_004840"
     CDS             complement(3629..4519)
                     /gene="dgcZ"
                     /locus_tag="ACHK01_004840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO8099633.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4774..5166)
                     /gene="ydeI"
                     /locus_tag="ACHK01_004841"
     CDS             complement(4774..5166)
                     /gene="ydeI"
                     /locus_tag="ACHK01_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO8099634.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1441 a         1260 c         1116 g         1443 t
ORIGIN      
        1 ttaaatatca tatcccgcag tagctatacg tcctggtaca ttgatcacag taatacagtg
       61 ataaagttgt tcaacatcag ggtgattatt aaacgactcg gccatacgat aaaccaatca
      121 gaactcaact ccttgttttc aatggctctt aaaaggtgca aaagtgattt ttcaaaagat
      181 tgcccgctta cttaaatccg aaatcaatgg caattcatgg catgttggtg atttgttgcc
      241 gtcagaagcg gaactcgctg ttcgttataa tgtttcgcgt aataccctgc gtaaggcatt
      301 atccttgctg gaaggcgaag gaattattca cagaaagcat ggttcaggaa catacattca
      361 aaaaaagaat tttgttgcac acattgatca catgaacagt ttcagtgaaa ttgcgcataa
      421 aagcggcaaa gaggcaggga gtcagattat gaaatttgaa gtgcaggatg cttcacccac
      481 tattgcgact gagctgaatt tagtgactgg tgagcaggtt tattacataa agagactgcg
      541 attcattgag gataatgcag cacaattaga agaaacgtgg atgtcagtag cacgttttcc
      601 tgatttaacc gtgtcacata tgcaaaaatc caaattttca tatattgaga atgaatgcgg
      661 gatcaaaatc attggcacct ttgaaacttt ctcccctact tttcctaccc cagaaatcgc
      721 cagtatttta cggatcagcc cacgggatcc catacttaaa attcagaccc aggctgtgga
      781 cagtaactct attccgctgg attattcgtt actttacagc aatattttcg agttccaggt
      841 aaagtacttt ttcccgcgat aaaagcagac gcacagagtg ctgacatcac attctgtgcg
      901 tatggtttag cctctaacct ttaccgcttt acgccaccgc aagccaaata cattgatata
      961 cagcccggtc ataatgagca ccgcacctaa aaattgcaga cccgttaagc gttcatccaa
     1021 caatagtgcc gcacttgcca gtccgactac gggcaccagt aacgataacg gtgcaacccg
     1081 ccaggtttca tagcgtccca gtaacgtccc ccagatccca taaccaacaa ttgtcgccac
     1141 aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca gactgtgaat
     1201 catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga ttgggattaa
     1261 agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca tgatcttttt
     1321 attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca taaagccgag
     1381 catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc caaaaatcgc
     1441 taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtac acgcgccaag
     1501 cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag caggcatacc
     1561 aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta atccataccc
     1621 caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg gaaaagcgac
     1681 cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc ccactttgat
     1741 gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc catcttttcg
     1801 cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc tgaatttact
     1861 tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg caaataagtc
     1921 gctatacttc ggatttttgc catgctattt ctttacatct ctaaaacaaa acataacgaa
     1981 acgcactgcc ggacagacaa atgaacttat ccctacgacg ctctaccagc gcccttcttg
     2041 cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg accatttact
     2101 tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca attgcgctca
     2161 ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc gacaagaaac
     2221 gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt cctttagtga
     2281 ataacgtgac gctggtggtg ctcttttttg ccctcattaa ctgcgcctat tctgtttttg
     2341 ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa acgaaaatct
     2401 tctcaatcaa ctacaccatg ctcaacattg gctggaccat cggtccgccg ctcggcacgc
     2461 tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt tccgcgtttc
     2521 ccatgctttt cattcaaatt tgggtaaagc gcagcgagaa aatcatcgcc acggaaacag
     2581 gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg tttacctgct
     2641 ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca caatatgtga
     2701 tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt ccggtgaatg
     2761 ctgccatggt ggttacgttg caatattccg tgggccgccg acttaacccg gctaacatcc
     2821 gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg tctggtcggt tttatttttt
     2881 ccggcaacag cctgctattg tggggtatgt cagctgcggt atttactgtc ggtgaaatca
     2941 tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga atgaaagcca
     3001 gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca ttagtgagtg
     3061 gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg ttggtgatca
     3121 ttgctgcgtg ggtgctgatg ttaaaaggga ttcgagcaag accgtggggg cagcccgcgc
     3181 tttgttgatt taagtcgaac acaataaaga tttaattcag ccttcgttta ggttacctct
     3241 gctaatatct ttctcattga gatgaaaatt aaggtaagcg aggaaacaca ccacaccata
     3301 aacggaggca aataatgctg ggtaatatga atgtttttat ggccgtactg ggaataattt
     3361 tattttctgg ttttctggcc gcgtatttca gccacaaatg ggatgactaa tgaacggaga
     3421 taatccctca cctaaccggc cccttgttac agttgtgtac aaggggcctg atttttatga
     3481 cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt gcatggatag atttgtgttt
     3541 tgcttttacg ctaacaggca ttttcctgca ctgataacgg atcgttgaca cagtagcatc
     3601 agttttctca atgaatgtta aacggagctt aaactcggtt aatcacgttt tgttcgtcaa
     3661 taaacatgca gcgatttctt ccggtttgct taccctcata cattgcccgg tccgctcttc
     3721 caataaccac atccagaggc tcttcaggaa atgcgcgact cacacctgct gtcacggtaa
     3781 tgttgatatg cccttcagaa tgtgtgatgg catggttatc gactaactgg caaattctga
     3841 cacctgcacg acatgcttct tcatcattag ccgctttgac aataatgata aattcttcgc
     3901 ccccgtagcg ataaaccgtt tcgtaatcac gcgtccaact ggctaagtaa gttgccaggg
     3961 tgcgtaatac tacatcgccg attaaatgcc cgtaggtatc attaaccaat ttaaatcggt
     4021 caatatccaa caacattaaa taaagattca gaggctcagc gttgcgtaac tgatgatcaa
     4081 aggattcatc aagaacccga cgacccggca atcccgtcaa aacatccata ttgctacgga
     4141 tcgtcagcaa ataaattttg taatcggtta atgccgaagt aaaagaaagc aacccctcct
     4201 gaaaggcgtc gaaatgcgcg tcctgccagt gattttcaac aatagccagc attaattccc
     4261 gaccacagtt atgcatatgt tgatgggcag aatccattag ccgaacgtaa ggtaattcat
     4321 cgttatcgag tggccccaga tgatcaatcc accgaccaaa ctggcacagt ccataagaat
     4381 ggttatccgt tatttctggc ttactggcat ctctcgcgac cacgctgtga aacatactca
     4441 ccagccactg gtagtgggca tcgatagcct tattgagatt taacaagatg gcatcaattt
     4501 ccgttgtctt cttgatcatt gccactcctt tttcacagtt ccttgtgcgc gctattctaa
     4561 cgagagaaaa gcaaaattac gtcaatattt tcatagaaat ccgaagttat gagtcatctc
     4621 tgagataaca ttgtgattta aaacaaaatc agcggataaa aaagtgttta attctgtaaa
     4681 tcacctctgc attatcgtaa ataaaaggat gacaaatagc ataacccaat accctaatgg
     4741 cccagtagtt caggccatca ggctaattta tttttatttc tgcaaatgag tgacccgaac
     4801 gacggccggc gcgcttttct tatccagact gccgctaatg ttgatcatct ggtccggctg
     4861 aacttctcgt ccatcaaaga cggccgcagg aataacgaca ttaatttcac cgctcttatc
     4921 gcgaaaaacg taacggtcct ctcctttgtg agaaatcaaa ttaccgcgta gtgaaaccga
     4981 agcgccatcg tgcatgtttt ttgcgaaatc aacggtcatt ttttttgcat catcggttcc
     5041 gcgatagcca tcttctattg catgaggcgg cggtggcgct gcatcctgtt ttaaaccgcc
     5101 ctggtcatct gccaacgcat aaggcatgac aagaaaactt gctaatacaa tggcctgaaa
     5161 tttcatactg actccttaat tgcgtttggt ttgacttatt aagtctggtt gctattttta
     5221 taattgccaa ataagaatat tgccaattgt tataaggcat
//
LOCUS       DAZGCS010000206         5235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGCS010000206 DAZGCS010000000
VERSION     DAZGCS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.209"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(224..443)
                     /gene="ydfC"
                     /locus_tag="ACHK01_004842"
                     /pseudo
     CDS             complement(224..443)
                     /gene="ydfC"
                     /locus_tag="ACHK01_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
     gene            complement(603..758)
                     /locus_tag="ACHK01_004843"
     CDS             complement(603..758)
                     /locus_tag="ACHK01_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8099635.1"
                     /translation="MDTIDLGSNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(960..1367)
                     /locus_tag="ACHK01_004844"
     CDS             complement(960..1367)
                     /locus_tag="ACHK01_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099636.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPNNQPAILTEDQKELLQLFDALPDS
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1444..1671
                     /locus_tag="ACHK01_004845"
     CDS             1444..1671
                     /locus_tag="ACHK01_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8099637.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1655..2206
                     /locus_tag="ACHK01_004846"
     CDS             1655..2206
                     /locus_tag="ACHK01_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099638.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFDDGAVDQRALGVNRVKIFERWKSIDTRDKREKFTALIPAIMAAIRISDFRLY
                     REITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCATMG"
     gene            2178..3218
                     /locus_tag="ACHK01_004847"
     CDS             2178..3218
                     /locus_tag="ACHK01_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO8099639.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVTEDEYTQKRE
                     SLKVGKIGTVNKNRTENGYVSAQNSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAVPPVFLDGLSEPIGKFPMTDNWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGAHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3211..3672
                     /locus_tag="ACHK01_004848"
     CDS             3211..3672
                     /locus_tag="ACHK01_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8099640.1"
                     /translation="MTEQQIRQVCRQCMDCCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPAGPTPIELLKQEYERRKAAGFV"
     gene            3705..4451
                     /locus_tag="ACHK01_004849"
     CDS             3705..4451
                     /locus_tag="ACHK01_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8099641.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELRKAGFVD
                     KSVYTWRVAYNNVQQEQPAPAELSEETTTATVAKISECDLTATVEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDKLPAEPKSVPVTENDGKAFPQ
                     SAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWERVCAALRELNKHRDMVAGICRKSGQ"
     gene            4448..4612
                     /locus_tag="ACHK01_004850"
     CDS             4448..4612
                     /locus_tag="ACHK01_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099642.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKARVKGISLILR
                     GNFHQSTKYP"
     gene            4617..5150
                     /locus_tag="ACHK01_004851"
                     /pseudo
     CDS             4617..5150
                     /locus_tag="ACHK01_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324330.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         1396 a         1134 c         1404 g         1301 t
ORIGIN      
        1 gcaaccaatg gaaataatgc gctttgatgc tcattttcgt actttttgcc ccgcttgcaa
       61 taataagttc tggatgaact caagataatt aaccgtttaa ataagttggg actatccaga
      121 tttttaaaga acgtgccgga tgctcacccg tgtccggcgc acgcactcca cctgacccgt
      181 agagaactcc ttaattacca accttatcct cgttggttag ctattaacgc gggtatgtaa
      241 tcattctggc aatgcttaat gccgctgctt tttccaaatt ggtgatatcc tgctccagag
      301 cggacagatt tttcagcctg cttagccctg gcttcattgg cccatttcag atcctgcgct
      361 gcattaattt tctggcgcat ccactcataa agttcatcat cggtatagtc tggcgcgatg
      421 atgacgggtt ctcgtttatg catactgatt cctcgcggtg ctgtttcgct tatcagccgt
      481 tagattttgc ctaactggaa agcgcctgtt taaactcact gaagctgaga gcctcttcgc
      541 cttcggcaag gtcttcgaag tattcttcgt aagccttttc catgattgtg tcgaaatcca
      601 tatcactcac ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt
      661 ttgcttttgg tatacgtcat cgcggtgaac gtgccgtcct ggtttgggaa cacgccgtac
      721 accagagatt cgttgctgcc aagatcgata gtatccatgc tgacctcatt tccccttaac
      781 gccggagtag cggaactgtt tgctgagaac accgtgcggt gtcttgatga gtgaaattta
      841 gaataaccta aggcatatgg tcaagattct tatgtagaaa aacctaagtt ttttggtgta
      901 aaaaacacaa gtgtttgaaa gtttgtgctt tttattacag ggtgtggaga aaaaaggggt
      961 tatttgtttg cgcttctttt gcgagcttta agtagttctt caaaaagttt gttgaaattt
     1021 tcaactcgag cacgcatctc tgacaacagg gccttttgct ctgagtcagg cagtgcgtcg
     1081 aacagttgaa gtaactcttt ttgatcttct gtcagaatgg ctggctgatt attcgggatc
     1141 ggttcgcctg gttgtttatc ttcatcccca aaaagaagcc aagtcggtga gcactgaagc
     1201 gcttggctca gtgcgaataa tcttttcccc gccggctgtg tttcatctct ttcccattga
     1261 gaaattgtta cgtgagccac tttgaccagc ttacctaatg cggcctgaga cagttttaat
     1321 tttttacgcc tatgtaagag gcgagcaccg aaggtttcgt ttttcatatt agggaattct
     1381 aatttttctt gacttaggtt tctctacgat ctagtttcct taggaaaatc taaggagctc
     1441 gatatgttga aaattgatgc tatagcgttt tttggcagca aaacaaagct tgccaatgcc
     1501 gcaggagtta ggttggcaag cattgctgca tggggggaac tggttcctga aggtcgcgcg
     1561 atgcgcctgc aagaggcatc cggcggggaa cttcagtacg accccaaagt ttatgacgaa
     1621 tatcgtaagg caaagcgggc ggggcggttg aacaatgaaa atcacccctg aacaggtttg
     1681 tgaggctctg gatgcctggg tatgccgacc aggaatgaca caggagcagg cgacgatatt
     1741 aatcacggaa gcattctggg ctctgaaaga acgcccgaac atcgatgttc aacgcgtcac
     1801 gtttgatgat ggcgcggttg atcaacgggc gctgggcgtt aaccgggtga agatattcga
     1861 acgctggaaa tctatcgaca ccagggataa gcgggaaaaa ttcacggcgc tgattccggc
     1921 aattatggcg gctatccgga tcagtgattt caggttgtat cgtgaaatta ctgacggaaa
     1981 aagcattacg tacatgatcg ccgggttaaa caaagaatat ggcgatgtgg tggagtcagg
     2041 gctacttttt gcggatccag ctgttgtgga acgtgagact gacgaactta tagaaaaagc
     2101 tattgctttc aagcgtgcgt atcgtcagca ataccaacat tactttgcag ataaacaaat
     2161 atctgtctgg ggttcgtatg agtatcgatg cgctacgatg ggctaaaaag gtgaaaaccg
     2221 gcagttcatc cagtaagtca gtattgacct ggcttgctga tatgtgcggt gccgatttgt
     2281 gtgcataccc gtctgtatct gcactggcag aagtaacgga actgaacaaa aagactgtgc
     2341 aggacagctt acgacacctg atggagattg ggttaattgt tgataccggt gagagaaaag
     2401 gccggacaaa acaaattgtg gtgtaccgac ttatcggtgt tgaagaaagt gttaccgagg
     2461 atgaatacac ccaaaaacgg gagtctttaa aggtaggtaa aattggtact gttaataaaa
     2521 acagaaccga aaacggttat gtttcagcac aaaacagccc caaaaacgga actctttgct
     2581 gcatggaaaa taaccaaaga cacccaaatt ttccatcaaa gacacccaaa aacggatcac
     2641 ggaacccaaa ggaacccaaa gatttaaacc ccacacataa cgcacgcgag agtgctccga
     2701 ccagtgagca ggaagttttg tcgttacagg cagtcccccc tgtattcctg gatggcctga
     2761 gcgaacccat cggaaaattt ccgatgaccg ataactggta tccgtcacgg gattttcgac
     2821 gacgggctgc gttgtggggg atggctttgc cggagacaga attcacacct gctgaacttg
     2881 ccgcattccg ggactactgg gcagcggagg ggaaagtgtt tacgcaggtt cagtgggagc
     2941 agaaattcgc ccgtcacgta aatcacgtca gggcgcaggt taaaccagtc agcaaggggg
     3001 taaaccatgc agcagcacca ggtggaaccg catcacgggc agttcaggaa attcgggcag
     3061 cacgtgagca gtgggaacgt gaaaacggat ttatcagcga cggaaacggc ctggaagctg
     3121 tgggagctca tgggggaggt ttattcgaac cgctggaccc agaagaacgg ggccgcacct
     3181 tcgaagctct ggattgcaca gattggcgcg atgactgagc agcaaatccg gcaggtctgc
     3241 cgccagtgca tggactgctg ccgggcgggt gaaacatggc ctccggacct ggctgagttt
     3301 gtggcgctga tttcggaaag cggggccaat ccatttggtc tgacggtgga tgctgtgatg
     3361 gaggagtacc gccgctggcg caatgagtcc tggcgatacg acggaagtga taagtacccg
     3421 tggtctcagc ctgtgctgta tcacatttgc ctcgagatgc gttcaaaggg gattgaacgc
     3481 cagatgaccg aaggggagtt aaaacggctt gcagaacggc agctgacgaa atgggcaaag
     3541 catgttagca acggcctgag tgttccgcct gtccggcgac aactggcagc acccaaacgt
     3601 cctgcgggac caacgccaat tgagttgctg aaacaggaat atgaacgccg gaaagcggct
     3661 gggtttgtct gatttgagaa gtaattttta ccgggaggaa atttatggag actgtttttg
     3721 acgcactgaa agcgatggga aaagccacgt cggtagagct ggctgcgcga cttgatatca
     3781 gtcgtgaaga agtgctgaac gagctgtggg aactgagaaa ggctggcttc gttgataaaa
     3841 gcgtatacac ctggcgtgtg gcttataaca acgttcagca ggaacagcca gcgccggcag
     3901 aactgtcgga agaaaccacc acggcaacag tcgctaaaat ttcggagtgc gatttaactg
     3961 cgacggttga acaacgcgga ccacaaacgg ctgatgagct ggctacgttg ttcggtacaa
     4021 catcacgcaa agtggcttca acgctggcaa tggcaatcag caaaggtcgt ctgattcgtg
     4081 taaaccagaa cggtaaattt cgttactgca tgccgggcga taagttacca gcagagccga
     4141 aatccgtgcc ggtaacggaa aatgatggta aagcctttcc tcagtcagca ggtgttgcgt
     4201 taccagtaca ggaagctgca acacaggaag atattaaaac agaaactgtg gctgatattg
     4261 tgcaatcgtt gccatcgttt actgaaacgc gagcggatga cctggtttta ccatcgctgc
     4321 atatggcaaa ccgcgaattg cgtcgggcga aaagtcatgt ccagaagtgg gagcgagtct
     4381 gcgccgcgct gcgggagctg aacaagcacc gagatatggt tgccgggatt tgtcggaagt
     4441 ccgggcaatg agcggatggt gcaggcctga aattatgata ctaacaatga aggtaaaatg
     4501 catcggcagt ctgattggtc gtagtgaggc ggcggtcagg atgaaagccc gggttaaggg
     4561 aataagcctg attctgcggg gtaattttca ccagtcaaca aaatatccgt agcgcgataa
     4621 cggtcaaaaa tcatggcgct gacacttttg tgccactgga gatgactgta cctaagttca
     4681 ggggagaaga acacgtccgg tgggatggtc gggccagatt taaagggcag gtcatggctc
     4741 cagccagtac gctggcaatg gaggctgcct ggctggaaat tgatatggga accacgccac
     4801 tcagggattt actgccggtc cagagaataa attcctgtta cggttacacc actgtgatct
     4861 tgcaagtgca ggaaagtagg tctacacggt aacgcgagtg cgtgtaactt ttgatgttat
     4921 ttccgtagaa acaccggaca aattttcgct ggcaggtcat gctgaaggta taaatctgca
     4981 gattatggac aattacggat atccggcaag agccggaaaa agcatgccgc ctctaattct
     5041 cagtggaaga tggacttgat tatactcatt gcattgtcag aaatagttat ccattaaagg
     5101 ctggctattc caaacaggat gttgattaca aaaatgtaat caacatgtaa ggtttatact
     5161 cttcaatatg cgtataattt tccttatttt gttaacttta aataacaagc tatgcacgag
     5221 gtaaagtcgg ataag
//
LOCUS       DAZGCS010000207         5217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGCS010000207 DAZGCS010000000
VERSION     DAZGCS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.210"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..460
                     /locus_tag="ACHK01_004852"
     CDS             <1..460
                     /locus_tag="ACHK01_004852"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HFO8099643.1"
                     /translation="WFAPIYFIESEGVYLKHPVLYCALYLVILFISPILLTIFFSWMR
                     TWRWLCNYMPHPTGRAWDYFFGLKVPCWMIITLKNGKKIAGKFGANSFASSAPEPEQI
                     YLEEHWVLNDDGGFERPRVSTLGILILGNDIENLEFFRFELPTCNNGESN"
     gene            460..603
                     /locus_tag="ACHK01_004853"
     CDS             460..603
                     /locus_tag="ACHK01_004853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099644.1"
                     /translation="MSDTKRSYKEDGYTPIEKGYQPKNDLANNGFQPSKQTSQPAPPP
                     KKP"
     gene            complement(604..885)
                     /locus_tag="ACHK01_004854"
     CDS             complement(604..885)
                     /locus_tag="ACHK01_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8099645.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKSPGKPPRPDQLRTHERLRKLGFTVVVLDSKELGGIIPPNT"
     gene            complement(875..1096)
                     /locus_tag="ACHK01_004855"
     CDS             complement(875..1096)
                     /locus_tag="ACHK01_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8099646.1"
                     /translation="MSFKYRDSPLYYRTAQEALRLEQSGEYDRAAKVWAKANRESRNE
                     LNQDWRERRADSCLMQNMREKRKAVDDGL"
     gene            complement(1162..1923)
                     /locus_tag="ACHK01_004856"
     CDS             complement(1162..1923)
                     /locus_tag="ACHK01_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFO8099647.1"
                     /translation="MMQDLNEMALKYPHPVETFRQFLVESGFDVTGEENKAGHDCYRL
                     SNGAIITLYHTGTILIQGSQAAKPEVEAIINEHLGNAPAQRVQPAPVQEPAKKIFIVH
                     GHDHAAKEQLELILHKLGLPDHFILQNTGGTGLTIIEELEREIGQGQTATRFGIVLLT
                     PDDVGYSKRDGQEAALPRARQNVVLEMGMLLSSLGRSNVVILQKQHLEQPSDAAGILY
                     LNFNDHVRETVPRLIQRLKQAGFTFNEAQIANASS"
     gene            complement(1977..4055)
                     /locus_tag="ACHK01_004857"
     CDS             complement(1977..4055)
                     /locus_tag="ACHK01_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO8099648.1"
                     /translation="MMQKLWLDLETFSEIPIKNGTHVYAEGVEIMLFAWAIDEDPVSV
                     HDLTTDYNLPTRLLTALSDESVLIYAHNSHFDRTMLRHALLHTPQCVVAGSAKRWRDT
                     MVQALAHGLPGSLGELCEILGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHP
                     EEWRRFVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAA
                     IEAVDQEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYSVELPDMQRSTLERRIAD
                     PDLPSAVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLF
                     QPQNLPRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSD
                     LSNIEGRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGK
                     VMELGLGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKST
                     FGLSERVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDG
                     AWLRIQLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARD
                     VLAGNMPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKVLSALLSTNPEWAPDIPLNAG
                     GFEAYHYRKD"
     gene            complement(4129..>5217)
                     /locus_tag="ACHK01_004858"
     CDS             complement(4129..>5217)
                     /locus_tag="ACHK01_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFO8099649.1"
                     /translation="SLIKTRKNIGSIITTNYDQLVENIFEFNPLIGNNILLSNPYGSV
                     YKIHGCVSDPNNIIITGEDYANFDNKYELIRAQLLSIFIHNPIIFIGYSISDKNIKYL
                     LKTIFSYVDLNTELAKRIKDNFLLVEYEKDSMSTEITEHDIDIEGMSIIRINKIKTNN
                     YKAVYDAISGLVLPVSAMDIRKVQKVWSTIKSGGEIKVKITENLDELRNDEMVIAVGS
                     ERTVQYVYQTKSEMIQNYFKIVDEANSQLIALLNKQTIANSEHFPIYAFSSICPELEN
                     TETYRKRQLDKIIGNLSRIKTCKSEAVSIDEILKETKEYKVANTIMYCVLNDTISLKQ
                     LRDYLTKPGHKIDTQYRRLLCLYDYLSY"
BASE COUNT         1294 a         1172 c         1192 g         1559 t
ORIGIN      
        1 atggtttgct cctatatatt ttatagaatc tgagggtgta tatctcaaac atcctgttct
       61 ttattgtgct ttatacttgg tgattttatt cattagccca atattgttaa cgatattttt
      121 ctcttggatg cgaacttggc gttggttatg taattatatg ccacatccta ccggaagagc
      181 ttgggattat ttctttgggt taaaagtccc ctgttggatg attattacgc taaaaaatgg
      241 taaaaaaata gcaggcaagt ttggggctaa ctcatttgct tccagtgcac cagagccaga
      301 gcaaatttat cttgaggaac attgggttct aaacgatgat gggggatttg aacgccctcg
      361 agtttcaaca ttaggaatat taattttagg taacgatata gaaaatttag agtttttccg
      421 ttttgaacta ccaacatgca ataatggaga aagcaactga tgtcagatac caaaagaagt
      481 tataaggaag atggatatac ccctattgaa aaagggtatc agcctaaaaa tgacttggct
      541 aataatggtt ttcaaccttc gaagcaaact tcccagccag ctccaccacc caaaaaacca
      601 taactaagta ttggggggaa taattccccc cagctctttg ctatccagta ccaccacggt
      661 aaagcccagc tttcgcagac gttcatgtgt gcgcaactgg tcaggtcgtg gtggtttgcc
      721 gggggattta cattcaacga aaacgatgcg accgccgggt agcagaacaa tgcgatccgg
      781 taccgagcgg cgaccgggag atacgaactt aaaggcaacc ccgccagctt ttttcacttc
      841 agcgacgaga tgcttttcga taaggctttc acgttcatag gccatcatcc accgccttac
      901 gcttttcacg catgttctgc atcaggcaag aatcagcccg gcgctctctc cagtcctgat
      961 taagttcgtt acgtgattca cggtttgctt tggcccagac cttcgccgcc cggtcatact
     1021 cgccggactg ctcaaggcgc aaagcctcct gtgcagtccg ataataaagc ggactgtccc
     1081 gatatttaaa tgacataggg gttacctcaa ataaataccc ctgcatttgc aggggtattt
     1141 actttttaga aaaatgattg attaagatga agcgttcgct atttgagctt cattaaatgt
     1201 gaaaccggcc tgtttaaggc gctggattaa gcgaggtaca gtttctcgaa catgatcatt
     1261 aaagttgaga taaagaatac ccgcagcatc agaaggctgt tcaaggtgtt gcttctgcaa
     1321 gatgaccacg ttactgcggc cgagtgatga taacaacatc cccatttcta gaactacatt
     1381 ttgacgagcc ctgggcaggg ctgcttcttg accgtctctt ttggagtatc ccacatcatc
     1441 gggagtgaga agaacaatgc cgaaacgagt cgctgtctgc ccttgaccaa tttcacgctc
     1501 cagttcttca attatcgtaa gtccagtacc acctgtattc tgcaaaataa aatgatcagg
     1561 taacccaagc ttatgaagaa tgagctcaag ctgctctttt gctgcatggt cgtgaccgtg
     1621 aacgatgaaa atctttttgg ctggctcttg aaccggggct ggctgaactc gttgagctgg
     1681 cgcgttaccc aagtgttcat taataatggc ttcaacctct ggttttgccg cttgtgagcc
     1741 ttgaatcaaa atcgttccgg tgtggtaaag cgttatgatg gcaccattac ttaaacgata
     1801 acaatcatga ccggctttgt tttcttctcc ggtaacatca aatccggatt cgactaaaaa
     1861 ttgacgaaaa gtttcgacag ggtgtgggta tttgagagcc atttcattga gatcctgcat
     1921 cattattttc ctataaaata agcaatattt agcaaagcag caatgacagc gagcagctaa
     1981 tccttacggt agtggtacgc ctcaaaaccg ccagcgttca gtgggatatc gggcgcccat
     2041 tcggggttag tggagagaag cgcggaaagc actttatcgt tgaaatcttc tgtgtcaggt
     2101 gcttcggtga tcacctcgtc gtgtaccgtc aacacaatgc tgtaaccggc atcttcgata
     2161 agcggcatgt ttccggccag aacgtcgcgg gcggccgcct gggtgacgtt ctccaccagc
     2221 tttccgccgt aggttttgag tcgttgccat ttacgcgaat aagagttaac acccatatag
     2281 gtgatattcc ctttttcgat aaccggagac gggtagcata cagcgcgtcc ggatggtagc
     2341 tgtatgcgca gccacgcgcc atcacgtcgg attttaagat aaccgcaata caatgttttt
     2401 tgcggtgtgg cgattgctgt gcggacagtg cgctccagct cgtaccagaa atcgcaggtc
     2461 gccgggtgcg ccctgcgcca caggcgcttg agcgagtcac atgcgatgaa tacacgctcg
     2521 gaaaggccaa aggtcgactt acgtttaacc gattcgtcgt accagctttt cgcctcgcgg
     2581 ataacatcgc ggggaatgtt tggcagtgcg gcgttcgcca gctcgtcgag atcgagaccg
     2641 taaaccagag caaaagtgat gaaagccgat acaccacctc cataacccag accgagttcc
     2701 atgactttac cgatctgacg catgtgttta tcaacatcat ctggtgcaat atcgaaagct
     2761 tttgcatacg ccagtttata taagtccgga cccgttccgg cgtcgtactc tctgaatgca
     2821 ttcagtttcc attcttctcc cgccagccat gccagcatac ggccttcaat gtttgacaag
     2881 tcacttacca ccagtttttt gcctgttggc gcgataatgc agccacgtaa cgctgaactg
     2941 gttagttcca tgatattgtc aaacagcagg tctgcacatc cggctttcag tgcttcgatg
     3001 ccttcgtcta tttgttcctg ttttagtgaa gggcggggaa ggttctgggg ctggaatagc
     3061 cgtccggccc aacgaccggt acgtgacgcc ccgcagaact gtagcgtacc gcgtaagcgc
     3121 ccgtcgtggc ttacgccttt catcagtgcc ttgtatttac tggtgctggt agtactggct
     3181 tgcaggcgga tagccagcag ttctttcacg gcagatggta aatcggggtc ggcaatacga
     3241 cgttccagag tactgcgttg catgtctggt agctccacac tgtaggattc aacaatgtgc
     3301 ttaatcaacg cgtcccgttg tgtggctgcc tgcacttcgc catcagtcat ttcctgtgta
     3361 cgctttgcca ggcgcttttg ctcctggtct accgcttcga tcgcagcgcg cgcgagttgc
     3421 atgtccatgc agacgccccg gtcgttgatc tgctgatcac gatgccagag cgccagctct
     3481 gtcccctgat aattccactt cggcagacgt ttatagactt cgcgcattgc ctcgatatcc
     3541 agtccggcgt aagcaacaaa gcgccgccat tcttccgggt gggttttgct ggtggcacgg
     3601 cgcagtttgc tgtttttcgg gcggggctta cagaacagct ggatcagcgc tttaccttct
     3661 ttgtccttcg ctttgtcttg cgggacgccg agtatttcgc agagttcccc cagagacccc
     3721 gggagaccat gcgccagcgc ctgtaccatt gtgtctcgcc agcgtttggc actacctgca
     3781 acaacacatt ggggagtgtg cagtaatgca tggcgcaaca tggtgcgatc gaaatggctg
     3841 ttgtgtgcat atattagtac gctctcatcg ctgagagctg tcagtagtcg tgtcggcaag
     3901 ttatagtctg tggttaaatc gtgtacgctg acggggtctt cgtcgattgc ccatgcgaat
     3961 aacatgattt cgactccttc tgcatagaca tgggtgccgt tttttattgg gatttcgctg
     4021 aaagtttcga ggtctaacca aagtttttgc atcaagtaag gacccgttgg atttaataaa
     4081 taaaaagccg ccaaaatggc ggctttttat aaccgaagtt aagaatgctt aatatgataa
     4141 ataatcatac aagcataaaa gtcgcctgta ttgggtatca attttatgcc ctggttttgt
     4201 taaataatct cgtagttgtt tcaatgaaat agtatcgttt agtacgcaat acataatcgt
     4261 gttagcaact ttatactctt tcgtttcttt tagtatttca tcaatagaca cagcctcact
     4321 cttacatgtt ttaattcgag aaaggttacc aatgatttta tctagctgcc gttttctgta
     4381 tgtctcggta ttttctagtt cagggcagat agaactaaaa gcgtatattg gaaagtgttc
     4441 gctattagct atggtttgct tattcagtaa tgcgataagc tgagagtttg cttcgtcaac
     4501 tattttaaaa tagttctgta tcatttcaga ttttgtttga taaacatatt gtacggttct
     4561 ttctgaacca acagcaatga ccatttcatc attgcgaagt tcatctaaat tttctgtaat
     4621 tttaaccttt atctcaccac cacttttgat ggtactccaa actttttgaa cctttcttat
     4681 gtccattgcg gatactggca aaactagtcc agagatggca tcatatacag ctttgtaatt
     4741 gtttgttttt attttgttta ttcttatgat agacatgcct tctatatcta tgtcatgctc
     4801 cgtaatttca gttgacatgc tgtctttttc gtattcaacc aaaaggaaat tatcttttat
     4861 cctttttgca agttcggtat ttaagtcaac gtatgagaat atggttttta ataagtactt
     4921 aatgttttta tcgctaatac tataccctat aaatatgata gggttgtgta tgaatattga
     4981 gagtaattga gcgcgaatta actcatattt attgtcgaag tttgcgtagt cttcacctgt
     5041 gataattatg ttgttaggat cactaacaca gccatgtatt ttgtaaactg aaccgtatgg
     5101 gttacttaaa agaatgttgt taccaatcag tgggttaaat tcgaagatat tctctacgag
     5161 ttggtcgtag tttgttgtaa tgatggagcc tatgtttttt cttgttttta ttaagct
//
LOCUS       DAZGCS010000208         5134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGCS010000208 DAZGCS010000000
VERSION     DAZGCS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.211"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHK01_004859"
     CDS             <1..375
                     /locus_tag="ACHK01_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO8099650.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            649..1344
                     /locus_tag="ACHK01_004860"
     CDS             649..1344
                     /locus_tag="ACHK01_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFO8099651.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1439..1570
                     /locus_tag="ACHK01_004861"
     CDS             1439..1570
                     /locus_tag="ACHK01_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099652.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            1793..1978
                     /locus_tag="ACHK01_004862"
     CDS             1793..1978
                     /locus_tag="ACHK01_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8099653.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPA
                     PDWQTPLNGIISPSESG"
     gene            2217..2768
                     /locus_tag="ACHK01_004863"
     CDS             2217..2768
                     /locus_tag="ACHK01_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8099654.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            2860..2991
                     /locus_tag="ACHK01_004864"
     CDS             2860..2991
                     /locus_tag="ACHK01_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099655.1"
                     /translation="MRDNQKRYTPGFTALTERGVVPHGRWSGAMVEETGRCVTSQAL"
     gene            3114..3440
                     /locus_tag="ACHK01_004865"
     CDS             3114..3440
                     /locus_tag="ACHK01_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO8099656.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(3572..3772)
                     /locus_tag="ACHK01_004866"
     CDS             complement(3572..3772)
                     /locus_tag="ACHK01_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8099657.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3814..4179
                     /locus_tag="ACHK01_004867"
     CDS             3814..4179
                     /locus_tag="ACHK01_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8099658.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            4468..5031
                     /locus_tag="ACHK01_004868"
     CDS             4468..5031
                     /locus_tag="ACHK01_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8099659.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5028..>5134
                     /locus_tag="ACHK01_004869"
     CDS             5028..>5134
                     /locus_tag="ACHK01_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099660.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHAL"
BASE COUNT         1302 a         1133 c         1442 g         1257 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtatcccgt cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaatcgaca gataagcaga atattttgct gaaaaatgac gttggccagc gcgggcggat
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agttaggcag attatttcac
      481 gcagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aatcaaccat gaaagagatg
      661 atttctgtcg atcatgaaat atccatgagt agtctggatt ttctgaataa cattattaat
      721 ccagcccggg cagaagccgg agaagtccct catgaaccgc gtaagtttct tgcaaaaatt
      781 gaggatgagc tagagcttga tggaaccgga aaaaaattcc ggttaaacaa taaccagaca
      841 agaacggcat actatgatct ggattttgac cagatgatgc tcgttggcat gagggagtca
      901 aaggccgttc gtcgttctgt gctggcaaga ctcaaagcga tgcatggtat tcagatcccc
      961 cggactttac ctgaggcgtt gcgatttgcg gcaaaactgg ctgaacagaa agcagtgctg
     1021 gaaaatcaac tggcaatagc agcgccgaag gctgaatttg ttgataacta tgttgaagca
     1081 tctggtctga tgggattccg ggaagttgct aagttactcg gtatcaaaga aaccgatttc
     1141 cggctgtttt tgttggagaa cggaataatg tatcgcctcg ctggaaaaat gacgccttac
     1201 tcgcatcacc tggatgcggg gcggtttagc gtgaaaacgg gcgaggcggg caatggtcat
     1261 gcttttacgc aggttaaatt caccccaaaa ggtgttcagt ggattgctgg tctgcttgct
     1321 gcatggagag ctaccgcagc atgaagatga taaaaatgga ctggaaattt ttgctggtct
     1381 ggctgattcc gtttttatgg gtggttgccc ggttaattac tgctattaag aggtaaagat
     1441 gtcagacaaa ctcataatgc tggcgaaggg cctttgtgta atcgtcggta tttcattttc
     1501 actaatgctg gttgctcttt ttctttccat aacctggatg gcgttgactt cggcagggct
     1561 ggtggggtga gcataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt
     1621 agtgcgctgt ggctggcaac agaccattac cgtgataacg ccatcaccta caaagcgcag
     1681 cgcgataaaa aagccagaga gctggagctg gcaaacgcaa ccattactga catgcagcag
     1741 cgccagcgtg atgttgctgc gcttgatgcc agatactcaa gggaattagc cgatgcgaga
     1801 gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcggatcaac
     1861 gccacctgct ccggtaccgt gcgtgaagcc accggcacct ccggaatgga taatgcaacc
     1921 ggcccccgac tggcagacac cgctgaacgg gattatttca ccctcagaga gcggttgatg
     1981 acaatgcaga agcaactgga aggggcgcag gaatatatcc gcactcagtg cctgaattaa
     2041 cagagccagc ttaatcgctg gctttttcat atctgaattt catcgcgcat ctcacgcgca
     2101 tatcacatcc ccgagccttt cagaaagttg agcctgagaa ctgtcgtata tggtggcgac
     2161 catctcgggg cggcttttct gtgagacagg ctcaattttc tgaaaggtaa gacgctatga
     2221 actatccgac gattgttaac ggtattgatt tccgcgatct tgtttttgtg acaggcaacg
     2281 agccggtgac ggatacccga aaggttgccg tagcatttgg caaagaacac aaagatgtat
     2341 tgcggaaaac aagagccgtt gttcaacaat gctcaaaaga atttgcagag cgcaattttg
     2401 cgctttgcta tgaaaacaat gagttacaga acggtaaacc ccagcccata tatcgaatga
     2461 caaaagacgg ttggacgatg ttggtatttg gttttacggg gaaagctgct atgaccttta
     2521 aggaagcgta cattcaggct ttcaactgga tggctgagct tattcagcaa ggtctggcga
     2581 atctggaggc ggagcgcaac gctgtaatgc tggagtacat gaaagagaaa gatgtcgcca
     2641 gtatgtcagg tcgtttgctc aatcgctggg gcagggtgaa gaagcctcag ttgctggcaa
     2701 ggctggacag gctggagcag caggggcaga ttgcgttacc cggatttgat aaaggcattt
     2761 cagcctgaca tagccatgcg ccgtatcgtc gccgtatcgt cgccgtatcc ccgcattaac
     2821 agagaccgca gcccgacagg gagactcctc tacgagagtg tgcgggataa tcaaaaacga
     2881 tacacaccgg ggtttaccgc gttaacggag cgcggcgttg tccctcatgg tcgctggtcc
     2941 ggtgcgatgg tggaagaaac cggacgatgt gttacctcgc aagctctgtg atgtcatgtg
     3001 tctgatttgt gatttaagtc ggataattgt cgttgccatt aagcagagga ttgatgaccg
     3061 acagggtggc attgttagaa taagacttat tcttatctgt gccgggaatg aaaatgaaaa
     3121 gaaatcttcc gttaattatt ttgttgtctt ctctggttat gggctgtacg caacataaaa
     3181 cagatatgcc ccgacagttg gttaaggcat taccacaata tccggcctat gcagcggcaa
     3241 attatataaa gggacgggtt gatgtgaagt ttgatattgg tgctgatggt actgtcaccc
     3301 gaattgagtt tatccgttca gagccgcacc atttgtttga tgagcaggtt gtaaaggcga
     3361 tggcaaaatg gcgatttgag aaggacaggc cgtgtaaagg cgtgaagaaa acgtttatct
     3421 ttagtccttc tgcaccctga ttatttcatc agaaattaat tatcactctg ttgttattct
     3481 gtacatcccg gcagggtaag tcttgttccg tcggatatga agatgaaata ttgttggagg
     3541 acagtgggta cctgctcctg taaccgaacg ttcatttctc gttatttgtc atgctggccg
     3601 ggcgcagatg cgttgcatct gttgccagcc ttctcctgca ggcttcaata acccacgctg
     3661 aaaagttacc ggacccttta tgctcaaggg ctatgttgat ttgttcaatt atgtgattgg
     3721 ggaaacggat attgcgggtt gtggttctgc gggtccggtt tttcgatgac attttctttc
     3781 ctctggtgac aagttatatg acggggattt tacatggctg agcttcgtac actccagagc
     3841 agaatcaaaa cactgaatac ccgacgggtg aatattctga agggggaaca gcgtcgtgtc
     3901 agtggcagtg cccgggtttc cctcaagcgt catatctggc tgagggatgc cgggcagtgc
     3961 tgtctctgtg gtcgtgtggt tgacctctgt gacagtgaac tcgatcaccg cattgcactt
     4021 cagttcggtg gtggtaatga ggagacgaat ctctggacgc tctgcaccga atgccatcga
     4081 caaaagtctg ctcgtgaagc ggcgggtggt atgccggacc cgacgctgcc ggaggtgtcc
     4141 ggaggtcatg gcagggcaga cgatatcatc ggactgtgac ccgccccggg ggggatcatc
     4201 cggcgaaaaa aacgatcgct ccggacaccg ccccccctct cacgcagaga aaaaattccc
     4261 gtttcagggc agttaacatg ttaactggct gtccgggcat ttttgcggtt tttatcttta
     4321 ttattcagtt tgttgtgcgg aaaaaatgtt aactggcttt ttcagcaaat gttaaccagg
     4381 cagcagttaa catttgcggc atgagacgcc gggaaaaatg ggctgaacca tacccggctg
     4441 agtgcgttct ggacccggga ggaggctgtg ctgacaacgc aaaaacgaaa atttgcgctg
     4501 gcgctcatgt ccgggaaaaa caaaacagcg tcagccattg ccgctggtta ttcggcgaag
     4561 accgccaggg ttaaaggctc gcagctggca aaagatccgg aggtgcttgc gtttatagcc
     4621 cgtaaacaat gcgagacggt ggaggtggat gaggttcctg tttaccggca gaaaaaatca
     4681 gagcaggagg ataaaccccg tcgccgtgag gcggctgcaa taccacagcc ggacgaaaac
     4741 aatccggaga tgccaccgtc cgcggtgatg tctcctggta ttgaatatat ggaggatggt
     4801 cttcccgatc cggtgaaagc gatggggcgt cttctggtgg agaacattaa taccgacccc
     4861 aggctggcgc tggatgcggc ttataagctg gcgcagttca cgcaccataa aaaaggggat
     4921 gccggtaaaa aatcggcaaa aggtgacgcc gcgaaaaaag cggctaaccg ttttgcggtg
     4981 ccaccaccac cccgcctggt ggtgaataat gataatgagg gcaacggatg atacctgtgt
     5041 ggagcacggc ctgcccggac tgggcagagc gcctgaaaaa ggggctgtcg attattccgg
     5101 ctccgattta tccggaccag gctgcacatg cact
//
LOCUS       DAZGCS010000209         5079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGCS010000209 DAZGCS010000000
VERSION     DAZGCS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.212"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(178..612)
                     /gene="merR"
                     /locus_tag="ACHK01_004870"
     CDS             complement(178..612)
                     /gene="merR"
                     /locus_tag="ACHK01_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFO8099661.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            684..1034
                     /locus_tag="ACHK01_004871"
     CDS             684..1034
                     /locus_tag="ACHK01_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFO8099662.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            1048..1323
                     /gene="merP"
                     /locus_tag="ACHK01_004872"
     CDS             1048..1323
                     /gene="merP"
                     /locus_tag="ACHK01_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFO8099663.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1359..1781
                     /gene="merC"
                     /locus_tag="ACHK01_004873"
     CDS             1359..1781
                     /gene="merC"
                     /locus_tag="ACHK01_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFO8099664.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1833..3527
                     /gene="merA"
                     /locus_tag="ACHK01_004874"
     CDS             1833..3527
                     /gene="merA"
                     /locus_tag="ACHK01_004874"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFO8099665.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3545..3907
                     /gene="merD"
                     /locus_tag="ACHK01_004875"
     CDS             3545..3907
                     /gene="merD"
                     /locus_tag="ACHK01_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFO8099666.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3904..4140
                     /gene="merE"
                     /locus_tag="ACHK01_004876"
     CDS             3904..4140
                     /gene="merE"
                     /locus_tag="ACHK01_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFO8099667.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4137..4784
                     /locus_tag="ACHK01_004877"
                     /pseudo
     CDS             4137..4784
                     /locus_tag="ACHK01_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          905 a         1641 c         1612 g          921 t
ORIGIN      
        1 accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc
       61 gaacggcctc accccaaaaa tggcagcgcc tacggcgctg actctttcgt tgaccaggcg
      121 caagcgcctg gaaatgctca attagggggc acctcagaaa acggaaaata aagcacgcta
      181 aggcatagct gaccttgcca ggcctgcttc gccctgtagt gacgcgatca acgggcagga
      241 aacattcccc tttcgtgcat ggcaggcgca cacgagttca gacagcacgg tttccatgcg
      301 cgccaagtcg gccatcttct cgcgcacgtc cttgagcttg tgttcggcca ggctgctggc
      361 ctcctcgcag tgggtgccat cgtcgagccg caacagctcg gcaatctcgt ccagactgaa
      421 ccccagccgc tgtgccgatt tcacgaattt cacccgaacc acgtccgcct ccccatagcg
      481 gcggatgctg ccgtaaggct tgtccggttc ccgcaacagg cccttgcgct gatagaagcg
      541 gattgtctcc acgttgaccc cggccgcctt ggcaaaaacg ccaatggtca ggttttccaa
      601 attattttcc atatcgcttg actccgtaca tgagtacgga agtaaggtta cgctatccaa
      661 tccaaattca aaagggccaa cgtatgtctg aaccacaaaa cgggcgcggt gcgctcttcg
      721 ccggcgggct ggccgccatt cttgcatcga cctgctgcct ggggccgcta gtactggtcg
      781 ccctgggctt ctccggtgct tggatcggca acctgacggt gctggaaccc tatcgaccgt
      841 tgttcatcgg cgcggcgcta gtggcgctgt tcttcgcctg gaagcggatt taccggcccg
      901 tgcaggcatg caagccaggt gaggtctgcg cgattccgca ggtgcgcgcc acctacaagc
      961 tgattttctg gatcgtggcc gtgctggtcc tggtcgcgct tggatttccc tatgtcgttc
     1021 catttttcta ttaaccagga gttcatcatg aagaaactgt ttgcctccct tgccctcgcc
     1081 gccgctgttg ccccggtgtg ggccgctacc cagaccgtca cgctagcggt tcccggcatg
     1141 acttgcgccg cctgcccgat cacagtcaag aaagcgctct ccaaggtcga aggcgtgagc
     1201 aaggtcgatg tgggcttcga gaagcgcgag gccgtcgtca cttttgacga caccaaggcc
     1261 agcgtacaga agctgaccaa ggccaccgca gacgccggct atccgtccag cgtcaagcag
     1321 tgagccagca agccaacgac aacagcgaga gccgcttcat gggactgatg acacgcattg
     1381 ccgataaaac cggcgcgctc ggcagcgtcg tttccgcgat gggctgcgcc gcctgctttc
     1441 cagccctcgc cagcttcggc gcggccatcg ggctgggctt cttgagccag tacgagggac
     1501 tgttcatcag ccgcctgctg ccgctgtttg ccgcgctggc cttcctggcg aacgcgctgg
     1561 gttggttcag tcatcggcaa tggctgcgca gtctgctcgg catgatcggc ccggccatcg
     1621 tgtttgcggc cacggtctgg ctgctcggca actggtggac ggcgaacctg atgtacgtcg
     1681 gcctggcctt gatgattggg gtgtcgatct gggacttcgt gtcgccggcg catcgccgtt
     1741 gcggaccgga cggctgcgaa ctccccgcca agcgcttgtg aaagacggct gaccgtgcga
     1801 cacggcggcc cacacgaata aggaacgatg gtatgagcac tctcaaaatc accggcatga
     1861 cttgcgactc gtgcgcagtg catgtcaagg acgccctgga gaaagtgccc ggcgtgcaat
     1921 cagcggatgt ctcctacgcc aagggcagcg ccaagctcgc cattgaggtc ggcacgtcac
     1981 ccgacgcgct gacggccgct gtagctggac tcggttatcg ggccacgctg gccgatgccc
     2041 cctcagtttc gacgccgggc ggattgctcg acaagatgcg cgatctgctg ggcagaaacg
     2101 acaagacggg tagcagcggc gcattgcata tcgccgtcat cggcagcggc ggggccgcga
     2161 tggcagcggc gctgaaggcc gtcgagcaag gcgcacgtgt cacgctgatc gagcgcggca
     2221 ccatcggcgg cacctgcgtc aatgtcggtt gtgtgccgtc caagatcatg atccgcgccg
     2281 cccatatcgc ccatctgcgc cgggaaagcc cgttcgatgg cggcatcgcc gctaccacgc
     2341 cgaccatcca gcgcacggcg ctgctggccc agcagcaggc ccgcgtcgat gaactgcgcc
     2401 acgccaagta cgaaggcatc ttggagggca atccggcgat cactgtgctg cacggctccg
     2461 cccgctttaa ggacaatcgc aacctgatcg tgcaactcaa cgacggcggc gagcgcgtgg
     2521 tggcattcga ccgctgcctg atcgccaccg gcgcgagccc ggccgtgccg ccgattcccg
     2581 gcctgaaaga cactccgtac tggacttcca ctgaagcgct ggtcagcgag acgattccta
     2641 agcgcctggc cgtgattggc tcatcagtgg tggcgctgga gctggcgcag gcgttcgccc
     2701 gactcggagc gaaggtgacg atcctggctc gcagcacgct gttcttccgc gaagacccag
     2761 ctataggcga agccgtcacg gccgcattcc gcatggaggg catcgaggtg agggaacaca
     2821 cccaggccag ccaggtcgcg tatatcaatg gtgaagggga cggcgaattc gtgctcacca
     2881 cggcgcacgg cgaactgcgc gccgacaagc tgctggtcgc caccggccgc gcgcccaaca
     2941 cacgcaagct ggcactggat gcgacgggcg tcacgctcac cccgcaaggc gctatcgtca
     3001 tcgaccccgg catgcgtaca agcgtggaac acatctacgc cgcaggcgac tgcaccgacc
     3061 agccgcagtt cgtctatgtg gcggcagcgg ccggcactcg cgccgcgatc aacatgaccg
     3121 gcggtgacgc ggccctgaac ctgaccgcga tgccggccgt ggtgttcacc gacccgcaag
     3181 tggcgaccgt aggctacagc gaggcggaag cgcaccatga cggcatcaaa actgatagtc
     3241 gcacgctaac gctggacaac gtgccgcgcg cgctcgccaa cttcgacacg cgcggcttca
     3301 tcaaactggt ggttgaagaa ggcagcggac gactgatcgg cgtgcaggca gtggccccgg
     3361 aagcgggcga actgatccag acggccgcac tggcgattcg caaccggatg acggtgcagg
     3421 aactggccga ccagttgttc ccctacctga cgatggtcga agggttgaag ctcgcggcgc
     3481 agaccttcaa caaggatgtg aagcagcttt cctgctgcgc cgggtgagga caaggaggtg
     3541 tgcgatgagc gcctacacgg tatcgcaact ggcccataac gctggggtga gcgtacatat
     3601 cgtgcgcgac tacctggtgc gcggcttgtt acggccggtg gcctgcacca cgggcggcta
     3661 cggcgtgttc gacgatgcgg ccttgcaacg gctgtgcttc gtgcgcgcgg ccttcgaggc
     3721 gggtatcggc ctggatgccc tggcgcggct gtgccgtgcg ctcgacgcag cggacggcgc
     3781 acaagccgca gcgcagcttg ccgtgctgcg ccagttggtc gagcggcggc gcgcggcgtt
     3841 ggcccatctg gacgcgcaac tggcctccat gccagccgag cgggcgcacg aggaggcatt
     3901 gccgtgaacg cccctgacaa actgccgccc gagacgcgcc aacccgtttc cggctacctg
     3961 tggggtgcgc tggccgtgtt gacctgcccc tgccatctgc cgattctcgc cgccgtgctg
     4021 gccgggacga ccgccggtgc cttccttggc gagcattggg gtgttgccgc gctcgcgctg
     4081 accggcttgt tcgttctggc cgtaacgcgg ctgctgcgcg ccttccgggg cggatcatga
     4141 cgagttcgca gcccgccgga tggacggcgg ccgagttggc gcaggcggcg gcgcgcggac
     4201 agcttgacct gcattaccag ccgctggtcg atctgcgcga tcaccggatc gctggcgcgg
     4261 aagcgttgat gcgctggcgg catccgaggc ttggcctgtt gccgcccggc cagttcctgc
     4321 cgctggccga gtcgttcggc ctgatgccgg aaataggcgc gtgggtgctg ggcgaggcct
     4381 gtcgccagat gcacaagtgg caaggaccgg catggcaacc gttccgtctt gccatcaatg
     4441 tgtccgccag ccaggttggg ccaacgttcg acgacgaggt aaagcgggtg ctggccgata
     4501 tggccctgcc cgccgagctt ctggagatcg aactgaccga atcggtcgca ttcggcaatc
     4561 cagccctgtt cgccagtttc gacgccttgc gcgccatcgg cgtgcgcttc gccgccgacg
     4621 acttcggcac cggctattcc tgcctgcaac atctgaaatg ctgccccatc accacattga
     4681 aaatcgacca atcctttgtc gccaggctcc cggatgatgc ccgtgaccaa actatcgtgc
     4741 gggcggtgat ccagctcgcg cacgggctgg gcatggatgt cattttcaga agacgactgc
     4801 accagttgat tgggcgtaat ggctgttgtg cagccagctc ctgacagttc aatatcagaa
     4861 gtgatctgca ccaatctcga ctatgctcaa tactcgtgtg caccaaagcg aggtgagcat
     4921 ggcgacggac accccacgga ttccagaaca aggcgtggcc actctgcctg atgaggcttg
     4981 ggagcgtgcg cgccgtcgtg cggagatcat cagtccgttg gcgcagtcgg agacggtcgg
     5041 gcacgaagcg gccgatatgg cggctcaggc gctgggctt
//
LOCUS       DAZGCS010000210         5013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGCS010000210 DAZGCS010000000
VERSION     DAZGCS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.213"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            367..3216
                     /gene="cah"
                     /locus_tag="ACHK01_004878"
     CDS             367..3216
                     /gene="cah"
                     /locus_tag="ACHK01_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO8099668.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            3327..>5013
                     /locus_tag="ACHK01_004879"
     CDS             3327..>5013
                     /locus_tag="ACHK01_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO8099669.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1282 a         1263 c         1446 g         1022 t
ORIGIN      
        1 tctgccaggg aaatgcctga taacattacg ttaaccaact gaaaacatga cacaaacccc
       61 tcatactcct cctgtttctc ttgcgtgacc gccttgtcgt taatagaata aaacgatcga
      121 taaaacaggt atcgatcgtt gctatcgatc gtttatagtg gttaatggtc tgcgaaaagg
      181 cagctgaatc tcttcatcat gcagaacgga attgcacaca acagactgat acttctctgt
      241 ctgatgaagg gggagagcgt ttacgcccgg gacgtatcgc tgtgcccgat aactctgttt
      301 tgtacctgcc ggtatccact tttgtgggta ccggcttttt tattcaccct ctgtaaggaa
      361 aagctgatga aacgacatct gaacaccagc tacaggctgg tatggaatca cattacgggc
      421 accctggtgg tggcctccga actggcccgc tcacggggaa aacgcgccgg tgtggcggtt
      481 gcgctgtctc ttgctgctgt cacatcagtc ccggcactgg ctgctgacaa ggttgtacag
      541 gcgggagaaa ccgtgaacga tggaacactg acaaatcatg acaaccagat tgtcttcggt
      601 acggccaacg gaatgaccat cagtaccggg ctggaactgg ggccggacag tgaagaaaac
      661 accggtgggc aatggataca gaatggcggg atagccggaa acaccactgt caccacaaat
      721 ggtcgtcagg tcgtgctgga ggggggaaca gccagtgata cggttattcg tgacggcggg
      781 ggacagagcc tgaacggact ggcggtgaac accacactga ataacagagg cgagcagtgg
      841 gtgcatgagg gcggggttgc caccggtaca attatcaacc gcgacggtta ccagagcgtt
      901 aaaagtggcg ggctggcaac aggaaccatc atcaacaccg gcgcagaagg cggccctgat
      961 tctgacaact cgtatacggg tcagaaggtc cagggaacag cagaatccac caccatcaac
     1021 aaaaatggac ggcagattat cttattttcc gggctagccc gtgacactct catttacgca
     1081 ggtggtgacc agtcggtaca cggaagggcc ctgaatacca cactgaatgg cggttaccag
     1141 tatgtgcaca gggacggact tgcgctgaac acggtaatta acgagggggg ctggcaggtt
     1201 gttaaggcag gtggcgctgc cggtaacacc accataaatc agaacggtga actgagggta
     1261 catgccggcg gggaagccac tgcagtcacc cagaacacgg gcggtgcact ggttaccagt
     1321 actgctgcaa ctgtcatcgg cacaaaccgt ctggggaatt tcacggtgga aaacggtaag
     1381 gctgacggtg ttgttctgga atccggcggt cgtctggatg tactggagag ccattcagca
     1441 cagaataccc tagtggatga cggcggtacc ctggcagtgt ctgccggcgg taaggcgaca
     1501 agtgtcacca taacatccgg tggtgccctg attgcagaca gtggtgccac tgttgagggg
     1561 accaatgcca gcggtaagtt cagtattgat ggcacatccg gtcaggccag cggcctgctg
     1621 ctggaaaatg gcggcagctt tacggttaat gccgggggac aggctggcaa caccactgtc
     1681 ggacatcgtg gaacactgac gctggctgcc gggggaagtc tgagtggcag aacacagctc
     1741 agtaaaggcg ccagtatggt actgaatggt gatgtggtca gtaccggcga tattgttaac
     1801 gcaggggaga ttcgctttga taatcagacg acaccgaatg ccgcgctgag ccgtgctgtt
     1861 gcaaaaagta actccccggt aacgttccat aaactgacca ccacgaacct caccggccag
     1921 ggcggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
     1981 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt ttacaaatgt cggaaacagc
     2041 aacctcgggg tggcaaccac cggacagggt atccgggttg tggatgcaca gaatggcgcc
     2101 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
     2161 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct
     2221 gaagtccccc tgtatacatc catgttgaca caggcaatgg actatgaccg gattctggca
     2281 ggctcccgca gccatcagac cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt
     2341 cagggcggtc atctcggtca cgataacaac ggcggtattg cccgtggagc cacgccggaa
     2401 agcagcggca gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc
     2461 ggtatgtctc tgacgacagg ggtgtatggt gctgcaggcc attcttccgt tgatgttaag
     2521 gatgatgacg gttcccgcgc cggcacggtc cgggatgatg ccggcagtct gggcggatac
     2581 ctgaatctgg tacacacatc ctccggcctg tgggctgaca ttgtggccca gggaacccgt
     2641 cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg gggctggctg
     2701 ggctcactgg aaaccggtct gcccttcagt atcactgaca atctgatgct ggagccacaa
     2761 ctgcagtaca cctggcaggg actctccctg gatgacggcc aggataacgc cggttatgtg
     2821 aagttcgggc atggcagtgc acaacatgtg cgtgccggtt tccgtctggg cagccacaac
     2881 gatatgacct ttggtgaagg cacctcatcc cgtgacaccc tgcgcgacag tgcaaaacac
     2941 agtgtgagtg aactgccggt gaactggtgg gtacagcctt ctgttatccg caccttcagc
     3001 tcccggggtg acatgagcat ggggacagcc gcagccggca gtaacatgac gttctcaccg
     3061 tcccggaatg gcacgtcact ggacctgcag gccggactgg aagcccgtat ccgggaaaat
     3121 atcaccctgg gcgttcaggc cggttatgcc cacagcgtca gcggcagcag cgctgaaggc
     3181 tataacggtc aggctacgct gaatatgact ttctgattat acggtatccc ggaaatacga
     3241 tttcaggaaa attcatgcag taatgggaaa ggacgctcat tgctgttcac cgcagttctg
     3301 aattattgaa tttataaagg tgaatgatgc acgaaaagaa catcgccctg ctttgtgatg
     3361 aagccgaccg gcttttgcaa ctgaatatca atctgctccg gcaaatggtt gatgagccgg
     3421 atgtgttatc tgacagtaag aacgaaaacg ggctgctttt tgataaacgg aaagcgctga
     3481 aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc ccgcagggag atggtgctgg
     3541 ctgtggtcgg cacgatgaag gcgggcaaat caaccaccat caacgccatt gtggggcagg
     3601 aaattctgcc caaccgtaac cgccccatga cctcggtacc gacattgatc cgccacgttc
     3661 ccggaaaaac ggaaccggtc ctccatctgg aacatattca gcccgtccgc aatttattaa
     3721 tcacactgca ggaaaaactc gccaccccgg cagggcagca agtcgcacag tccctgcagc
     3781 aaaccgggga tacccgcgaa ctactggata ttctggcgga tgatgtctgg cttaaaaatg
     3841 aataccacgg ggaagacgaa atctttaccg gactggcatc gttaaatgac ctggttcgtc
     3901 ttgctgcggc aatggggact gaatttcctt ttgacgaata cgcagaagtg cagaaactac
     3961 cggtgatcga cgtggcgttc agccacctgg tgggaatgga tgcatgtcag ggaacactca
     4021 cactgctgga tacccccggc cctaatgaag ccggacaacc acagatggaa atgatgatgc
     4081 gggatcaact acagaaagcc tctgcggtgc tggcggtgat ggattacacc cagatgaact
     4141 cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat tgctgacgtc tcagccggtc
     4201 gtctgtttgt gctggtcaat aaattcgatg aaaaagatcg caatggtgat ggtgcggatg
     4261 ccgtacgcca gaaggtgcca gcaatgctga acagcgatgt gctgcccgcc tcccgcgttt
     4321 atcccggctc atcacgtcag gcgtacctgg ctaaccgtgc gcttcatgag ttacgaaaaa
     4381 acggtgcgct tccggttgat gaagcctggg tcgatgattt tatcagggaa gctttcggac
     4441 ctatggtgga agaggacgac tggaaagaca gcactaaggt aaataaaaag gcagaaaaac
     4501 tctggaatat atctctgatc gatcaactga taacggaagt catacagagc tcccattcca
     4561 gagcggcggc actggcggtt gactctgccg ccgcaaaact gatgcagaat gcagaaaatg
     4621 tcagcgaata tctgtcatta cgtcatcagg ggctgcagca aagcattcag tcactacagg
     4681 cgcatattac cagcctgctg gcggatatcc aggagattga ggaatgtcag aatcaggtga
     4741 ccggtgatgt cagaatggcc atggaggaca tcaataccaa aaccggggaa ttactgacga
     4801 aggtctgtgc ctcactggag gaggagctga acgactattt cagaagcggt aaacgcaaag
     4861 aacagcaaat gctggaggaa gaaaattcag cgcagccccg ggagagaaac gcattcgctt
     4921 tctttcacga tattttcgga accggaaatc agcatgacag gatgcgggac tttgatccgg
     4981 acagccccga aataaaattc agtgatcgca ggg
//
LOCUS       DAZGCS010000211         4976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGCS010000211 DAZGCS010000000
VERSION     DAZGCS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.214"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHK01_004880"
     CDS             complement(<1..71)
                     /locus_tag="ACHK01_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099670.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSG"
     gene            complement(86..619)
                     /gene="casB"
                     /locus_tag="ACHK01_004881"
                     /gene_synonym="cse2"
     CDS             complement(86..619)
                     /gene="casB"
                     /locus_tag="ACHK01_004881"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO8099671.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(616..2178)
                     /gene="casA"
                     /locus_tag="ACHK01_004882"
                     /gene_synonym="cse1"
     CDS             complement(616..2178)
                     /gene="casA"
                     /locus_tag="ACHK01_004882"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO8099672.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(2276..4975)
                     /locus_tag="ACHK01_004883"
     CDS             complement(2276..4975)
                     /locus_tag="ACHK01_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO8099673.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLI
                     GSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQ
                     QEAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
BASE COUNT         1258 a         1314 c         1117 g         1287 t
ORIGIN      
        1 ccggaatcat cacggttaag attggcagca gggtaagcgg ttaacaaatg aagctgaata
       61 aatgttgtca tgagatagtc ctttattaat tttgttcgtt atcggcgtcg ccagcctggt
      121 aatattccag cgcccagcgg atacggataa attctgttgg tcgctgctgg cggcggtgat
      181 gattgagcag gtcatcgctc tcctggcacc agcggaagac cccctctgcc agagagtcaa
      241 gattgacgga accgtttaat aatttgaccg cacggcgtaa ctgccgcaat aaatcatccg
      301 gcgtttttac tgccaacaaa cgagtaaacc gccccttttt cattaccgcc gccagttgcg
      361 cggcaaaagg ctgccgttcg tcaatagctt taacattggc gctaactgct gctatcagcg
      421 ctagtgcggt gatacgccac tcgggttcat cctgccactt tatttgtctg ttcttaagaa
      481 acaggcgaaa tccatccgtc agacaaacat cattaaccgt cgtactacgc cgcagactgg
      541 cacgctcgcc gcgtttatcc tgcaactctt catgccattt acgcagcgca actttatgct
      601 catcttttac aatactcatt cagcagcctc ctgctttttc tccctggcgg cgttagcatt
      661 ttgcttctcc gctgatgttg taaaatattt cttgcgtgcg gtcatgacgc gttccaaatc
      721 aacgggctca tatggattgg taaagacacg atcgtcaaaa tcctgacgcg cgaataacca
      781 catttccttt tgccatttac tgagtaattc gtacggatcc tgaccttctt caattttgcg
      841 cactaatctc aggaatcgat gctgagtttt gttccagaag tcaatatcca caaaaccgaa
      901 atcaagcttt gtggttttct tctcagagaa ccatgcttct ttcaacgctc ggtgtaacaa
      961 gcttagaatg tgtgatgccg cttgcgctgc cagacgcaac ttaggtatta ggtcttcttt
     1021 tttgtttaac aacagcggga aatggtgttc ataccaacaa cgaaccttca tatcttcaaa
     1081 atcaaaacca aatccccata agcccaccct tgtctgcttt agattgctgg cattcaaaag
     1141 tttcacgacc tgtgcgggga gttcagtatt gttttttgag ttacctacct ctatgaggcc
     1201 taaccagtcg cgccagatta aaccaccagg ttgtggttta actgagtaaa aatctccacc
     1261 ctctttaagc ggcagacggt aaggtgttag aggatggcgc cacatgacgt attgaacacc
     1321 atagtttttg agtgacatca agcccaaaag agcatcgctc tgcctaccgc aaatgtcgca
     1381 gttacctatt gaggttgtct taaaatcaat acgtatacgt cgtggcattc cccagtaggc
     1441 ctgaagttta ttgacttgtt catccgttac cacagcaccg tccagttcgc tggtacgcgt
     1501 cggtgcaagc catggaaaga ccaggtcatc aaaggttttt ggtaaaggta aatcggcttc
     1561 atcttgcggc attacgttta gccacagctt tcgccacaaa ggggtttgtt gattgccctg
     1621 atactccagc aattctataa gtgtcgtcag aggccctccg ccacgtagcc caggatagta
     1681 accgcgtcca cctataggtg cattcaactg gattgaaaaa agcgctaaag gcacacaatg
     1741 caggcacaga tgctgagttg tgtctcgctt aataaaatgg tctgtatttc gttcttttgt
     1801 ttgttttcct ggtgcgtcag gaagaagcga agcaatagaa gttgccttaa cttttaattc
     1861 atcaaaatcc tgcataaatg aaggggaatc tggcccaaac tgaaaagcat gttctaaaga
     1921 cagcaatgct tcccttagct tttcagcttc cagtccgtct tcccagatat catcccaacg
     1981 acgttgatct tttggcgcga agctggtttg tagtagcccc aacaaaaact gccaggccgc
     2041 cccctggaga tctgcccgcg gcgcagcgat atcgacaaca ttttcatcag ccagatcgac
     2101 tggtgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg gggttgtcag
     2161 aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg tcggttgtcc
     2221 atccttccat ccttttctgt atatcaacta ttcagagagt cctgtcgcag acatctcaac
     2281 caaccttgcc aatcaatccc tccctggccg aatacccgca actttcgtca tcggtgacta
     2341 aaatcacgtt tgccatttcc ggatcttgcc gctgttcaac gcaccactgc ctgaacgcat
     2401 ccccttccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg cgcactctga
     2461 caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca ccgtcaatac
     2521 aggtagccag ccacagggaa acagactctt cagccagacg tgtcgacagt ttttccggaa
     2581 ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacatac tttttagccc
     2641 ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca aatccttccg
     2701 gcattaccac gtcctcaccg taaactgatt caataagaag ccgggctgcg tgtggcatct
     2761 gaatagcgcc ttgctcacgc agcacacgct gcgtcagcca gattcgccca tgatcgggat
     2821 agacatatgc actgttacgc atggcactgc cgaaccattc gtcaccagga gcgtcatccc
     2881 agacgggggc cagaatcagc aattcagggg gggaacgctc gtcttttccg tcacgcttta
     2941 actgaccatt aatatcgcgg atatgccgct gtaatcgccc cgctcgctgg attagcaaat
     3001 caatgggggc caagtcggag atcatttcgt ccaggtcaat atccacgctt tgttcaatta
     3061 cttgtgtaca aacaatgact tgtgacgctc tctgtaaact acaatattta ccaaagcgtg
     3121 ccagcgtttc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta tgaaaaagtg
     3181 aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatacacc tgaatagcgt
     3241 catcaacaga attccgaatc caggcgatgc atttcccctg acttaccgcc gattcgatac
     3301 gcgcaataca ctcttgttca ctatggagcc aacccacgct cacgctacgt tcaacttctt
     3361 tgcgcgtcgc tacccggtgc gaatgtacat cggttttcgt gacatgcgtc agccaggggt
     3421 aatcatcttt tcccaggagt ggggcttctt gctggccctc tgcaccacgc gcaaaggcgg
     3481 cgacgagttt gtcgcgctgc tgttgagata acgtagcaga aagcaaaata acgctgtttc
     3541 cgccacgcgc ctgccgctcg atcagccctt caagaatgca cgacatgtaa gcgtcacagg
     3601 cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc agattattat
     3661 gtttaaacgg catcactgcc atcatcgcct gatccagggt gccgacgcca atttcagcca
     3721 gcaacgcctt cttgttactg tcggcaaacc aggccgcaca accctggctg aaagcttgtt
     3781 catccggttc ttctgacccg attaaatcac cggaccagag tgattcatta aagcggtcca
     3841 ttaatgtgcg ggcactgtgt gccagcacca ggctggggcg agattctggc gaatagaaag
     3901 aaagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt gttggcagac
     3961 caaaaaacag cccctgagct ttccccgcag ctatcaacct gtgcgccagg ataagcgccg
     4021 cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt cctggctggc
     4081 tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata aaaggaaaca
     4141 gtgtattaat tccggtgaaa ggtgcggttt ctgcttttgg aggaaagacg gttaatgcgt
     4201 tttgagcctg cactaaagcc ttctgccagt aatgtttaat atccattgcc tgtgcgacgc
     4261 gtggaaaaaa tcgcgttgac gaacctgtcc agtctgcgag tacgacggtt gcagagatat
     4321 accaggaaag gtgttttata agttcaatgc cctcgtcatc atcccagaat gcggggatct
     4381 ctattcgcgg aaacagtgcc ttgattgcaa ggagaaaatc tcgtgctgca gctttgtctt
     4441 caggcagaaa attatccaat tcatccatac gatcaggggg ccgaccatga tgccctgtcg
     4501 tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc gaagatgaag
     4561 gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc agcgttgaat
     4621 gagagatttt ttcgtaattt tttctggcac tttcctgaat cttgagttca gggggcaggt
     4681 aaagttgttg aaaagagcga gcaaattttc caatatcgtg ccagcacagc aaccaggcga
     4741 aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa tcagccagcc
     4801 cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc gaatgccagc
     4861 aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt ccccagaaat
     4921 caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta cgcata
//
LOCUS       DAZGCS010000212         4904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGCS010000212 DAZGCS010000000
VERSION     DAZGCS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.215"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..1189
                     /gene="hemB"
                     /locus_tag="ACHK01_004884"
     CDS             215..1189
                     /gene="hemB"
                     /locus_tag="ACHK01_004884"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO8099674.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1295..2146)
                     /gene="tauD"
                     /locus_tag="ACHK01_004885"
     CDS             complement(1295..2146)
                     /gene="tauD"
                     /locus_tag="ACHK01_004885"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO8099675.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2143..2970)
                     /gene="tauC"
                     /locus_tag="ACHK01_004886"
     CDS             complement(2143..2970)
                     /gene="tauC"
                     /locus_tag="ACHK01_004886"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO8099676.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2967..3734)
                     /gene="tauB"
                     /locus_tag="ACHK01_004887"
     CDS             complement(2967..3734)
                     /gene="tauB"
                     /locus_tag="ACHK01_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO8099677.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3747..4709)
                     /gene="tauA"
                     /locus_tag="ACHK01_004888"
     CDS             complement(3747..4709)
                     /gene="tauA"
                     /locus_tag="ACHK01_004888"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8099678.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDYAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1147 a         1412 c         1248 g         1097 t
ORIGIN      
        1 agcacaacca aacgccaaaa aataataacc aagagctata aataaagatt aaaaatattt
       61 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
      121 ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct tcaggtatac
      181 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      241 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      301 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      361 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      421 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
      481 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      541 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      601 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgca actctggaaa atttaggcaa
      661 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
      721 cggccaggta caggcgattc gccaggcgct ggacgctgcg ggatttaaag atacggcgat
      781 tatgtcgtat tcgaccaagt tcgcctcctc cttttatggc ccgttccgtg aagctgccgg
      841 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
      901 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      961 tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
     1021 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1081 tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg cggatctgat
     1141 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1201 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1261 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
     1321 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1381 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1441 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1501 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1561 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1621 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
     1681 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
     1741 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
     1801 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     1861 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     1921 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     1981 gctgcggcgt aatagtttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt
     2041 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2101 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2161 ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa aggcgataat
     2221 cgcaatcacc gcgattcccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg
     2281 aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2341 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2401 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2461 ctgctgcgcg cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa aaatcgctaa
     2521 atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
     2581 ataagccagc ggcggcacgg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
     2641 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
     2701 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
     2761 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa
     2821 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
     2881 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
     2941 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
     3001 cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac
     3061 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac acggccaggg
     3121 ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
     3181 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt ttgcatctgg
     3241 tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag ctggggattc
     3301 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagatg
     3361 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
     3421 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
     3481 cgccacggta gtagcccttc attctgaaaa actacgccac gctctgctcc cggtccctca
     3541 atacgcttac ccgccagtag aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
     3601 ttcagcaacg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
     3661 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc gtaaagatga
     3721 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
     3781 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
     3841 atcgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
     3901 ttccccttca ccagccccgg aacgtcacct tcaggcacgc cgcttaaacg cgccagtttg
     3961 ctgatgtttt ccggctgttt cagccacacg tctgggttag caatgtacgg ttgctgagca
     4021 tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc ataatctttg
     4081 cgcaccaccc agacgtccag cgttggcgcg ccccactgcc cgacctgttc agaatcggtc
     4141 aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
     4201 atatctcccc gctgccaggc agcgataatc gcgggcggct gcaggttcac aatctccact
     4261 tgcccgggtt taatgcccca gtgtttcagc gccgccagca ggctgtagtg ggtggtggag
     4321 ataaacggta cggcgatgcg tttgccaatc agatcttccg gtttgctgat agttttcttt
     4381 accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc aatcggcacc
     4441 tgttggctgg ctgcaaccgc taacgggctg gaaccgaggt tgccgatttg cacatcgcct
     4501 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacgctcgcc
     4561 ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
     4621 tgataggcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggct
     4681 agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgggag
     4741 cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt
     4801 attcttagca aaaaaacggc taaggaaaga ttaaatatgc aattactccg ttctaacaat
     4861 tgggaaaatt actacacttc attttagtga tgcataaaca acca
//
LOCUS       DAZGCS010000213         4868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGCS010000213 DAZGCS010000000
VERSION     DAZGCS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.216"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHK01_004889"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHK01_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            417..1313
                     /locus_tag="ACHK01_004890"
                     /pseudo
     CDS             417..1313
                     /locus_tag="ACHK01_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            1264..1965
                     /gene="traT"
                     /locus_tag="ACHK01_004891"
     CDS             1264..1965
                     /gene="traT"
                     /locus_tag="ACHK01_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO8099679.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(2013..2264)
                     /locus_tag="ACHK01_004892"
     CDS             complement(2013..2264)
                     /locus_tag="ACHK01_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8099680.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2288..2578)
                     /locus_tag="ACHK01_004893"
     CDS             complement(2288..2578)
                     /locus_tag="ACHK01_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8099681.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            3264..3623
                     /locus_tag="ACHK01_004894"
     CDS             3264..3623
                     /locus_tag="ACHK01_004894"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO8099682.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            complement(3716..>4868)
                     /locus_tag="ACHK01_004895"
     CDS             complement(3716..>4868)
                     /locus_tag="ACHK01_004895"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8099683.1"
                     /translation="MFASLLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFK
                     YVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFT
                     KKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLL
                     WIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIV
                     FHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLI
                     DKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVS
                     SKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
BASE COUNT         1453 a         1060 c         1001 g         1354 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacaaca
      421 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
      481 ttcccattga agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg
      541 tacagcatga accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg
      601 tggcacatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
      661 agaatggcat catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac
      721 gctgtaagca gaaacgcaag ttcagggcga ctacgaactc gaaccacaat ctgccagttg
      781 cgccaaatct gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg
      841 acctgacgta tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt
      901 atacgtgcga aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta
      961 aagccctgtt tatggcgctc aggagccagc gcccacctgc cgggctaatc caccactctg
     1021 atcgaggttc acagtactgc gcatacgatt accgggtcat acaggagcag tttggtctga
     1081 aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc tccgatggaa agcttctggg
     1141 gaacgctgaa aaatgagagc ctgagccact atcgttttaa gagccgggat gaggccatct
     1201 cagtaatacg ggaatacatt gagattttct acaatcgtca gcgtcgtcac tctcgtctgg
     1261 ggaatatctc cccggcagcc ttcagggaaa aatatcatca gatggctgct taaaaaaaga
     1321 acaaatggta gtgtccgcta ttgtcagtac acctcaacca tctggcttga acctgcgagc
     1381 gaacgcaccg ttttcctgca gatcaaaaac acgtcggata aagacatgag tggactacag
     1441 ggaaaaattg ccgatgcagt caaagccaga ggttatcaga tcatgacttc cccggataaa
     1501 gcttactact ggatccaggc taacgttctc aaagcagaca aaatggatct gcgtgaatct
     1561 cagggatggc tgagtcgcgg ttatgaaggt gcagcagtcg gagctgctct tggaggtgga
     1621 atcacagcgt acaataccgg gtcggcaggt accactctcg gtgttggact ggctaccggt
     1681 ctgattggca tggccgcgga tgcaatggtg gaagatataa attacacaat gattacagat
     1741 gttcagattg ccgagcgaac cagaaccagc gtcagaacgg ataatgttgc agctttgcgt
     1801 cagggaacat caggctccaa aattcagaca agcacagaaa ccggcaacca gcataaatac
     1861 cagacccggg ttgtatccag tgccaaccag gttaatctga agtttgaaga agctaaacct
     1921 catctggaag accagctggc aaaatcaatc gccaatatac tctgatataa atatcccggt
     1981 ggccattcac ctggcctccg gccctcttag tctcaacgtt cagacaattc agatattatt
     2041 ttccctgtca caccatatac ccatgccgga acagaatcta atgacggtac cagtaaattc
     2101 ttcagcgttc ggtttgcctg acgcccgtag ctgtatttgt gtctgtttct tcttatacaa
     2161 gagcagtagt acaatttctc ttccagagtc agttcaactt taggacgtaa ccaataaaga
     2221 tctcttccac tataaaatgg tgaatcctgt acaaacgatt ccataacaga acctcctgct
     2281 gctacagtta acacagctgc tggcatggac taataccatc ttttaattta accctggcgg
     2341 agacgccatt atttttggac gttcttgaaa caaaattaat tcctgaagaa tctttctcca
     2401 gtttattcag ctcaagattc agtccataat taacgtcaaa taactctgat aaaggtatca
     2461 gtttatgttc aggataatga ttatcccgac gacaacaaat attaaaatcc tgcatattac
     2521 ccgacatcag gagttcaaaa ccgtccccgg catcattcat actggcagat aaattcatta
     2581 cccagtttag tgctaatcaa caggtacggc ttaacttaca ggaattcccc gggaggggcg
     2641 gactcatcct cagttagcgg caaaatcgca tcacagccaa tcgcctgtct cgattcccca
     2701 tcggcgaaca atgtatgtgg ccacaaaatc ctggctggct cagaacgaag agcatctgag
     2761 caaagaggtc atcaatctgg tatgaatatc tggcagaaaa tatcccctga atccacaact
     2821 gctgtgcata tctgctcaaa tcagcatcaa tcataaacca gtaatcacag tgcctgtgat
     2881 tgggttacag gaataagtcc cccttcataa ggcatatcat caataccggt gaaggaaaag
     2941 aaagcatcag atggcctgat agtctgcgtc agtcaatgac atcagtgctt tatccggaac
     3001 aatgtatcca tgttggcgac agagaaagcc attgccatga acagttttgt ttatccccgg
     3061 aaagtcattt ctgttacaaa tttatattga caaacttgct ttcgatgaga aacatactat
     3121 ttcaatgaaa tgcagaatga ggcaaaattt cgttatgttt tttaattccc gaaagaataa
     3181 atgtggctta ttacaaaatc ctgttaatgt taactgatat tttataatgc atattcagac
     3241 atataattaa ataagaggta tttatggcta atacgaccac tggtgttacc agaattatta
     3301 aagcagcggg ttattcatgg aaaggcttat gtgcggcatg gcgctatgaa gcagcctttc
     3361 gtcaggagtg cattctggtt ctgtcagcac tgataatatc cttttggctg gatgttgact
     3421 ctgttactcg agtattactc attggttcgg caatgatggt aattattgtt gaaataatta
     3481 acagtgcaat tgaagcagtt gttgacagaa ttggttcaga acatcacgaa ttatcaggca
     3541 gagcaaaaga tatgggttca gccgcagtgt tgattacgat tttagttgct atcattatat
     3601 ggggagtttt attactttca taacgcccaa catctaaatt attcacagtt acctggttaa
     3661 tatttactta aggataagag gtattcacct cttatcctca gaaaaatgtc agtatctatc
     3721 tgcaggagga tataatatca tcttctttat tataaactga agtatttaca ttccataacc
     3781 ccagtaacga tgaaaatata ttatcatgac ttactgttct gtttagagca tgttctgaca
     3841 aacaattcaa atcaattttt ttagaggaaa cgaaccccgg agacatccag gtaataagag
     3901 gcacatgcgt ttgctcagct ggagcaacat tatatggtgt tccatgtaag tataatccat
     3961 tttctcccag agattctcca tggtcagaga catataataa aacagtatca tactcatctt
     4021 tatattcgat aagcttgtca attaattttg atattatata atcagtatag cgaattgtat
     4081 tatcataggt attaatcaat tgctcatgcg tacaattttc gatatcacta cgatcacatg
     4141 ttggtttaaa atatttatgt ggctcaggat aacggagata gtaggtagga ccatgactac
     4201 ccatcaaatg aaatacgata agtttatcct ctgagttatt atttatatac tcatcaatat
     4261 tttttagcat aacctcatca taacatgagt ttttcttgca taatgtatta tctgaatttg
     4321 atggctcaat attaattgta gggattcgct tacatacacc tttacatccc ccgtcatttt
     4381 caatccagag taaatttacc cccgttctgt acagaatatc cagtacattt tccgaattta
     4441 ccgctttgcg ggaattgaac tcttttcgct tcatatctga aaacatgcat ggaacagata
     4501 tggctgtgga tgtgccacac gactgcacat tatgaaagga tataagttca ttatatttct
     4561 ttgtaaaatc attggtacca cgagaatatc cattcagagc atagttttga cttcttgctg
     4621 tctccccgac aactaaaaac ataattaccg gcttttgttt ttcatttatt gtgcgggatg
     4681 catccgttcc agtctgacga aactcgccag gagaaacaaa aaaatccctt acatatttaa
     4741 acccactgta gatataatta gtaggtatta tttctttatt taatgaagag ttgtttctgc
     4801 caactgatgc ataatcctta taaaagaaaa acgcaatcgc aaatataatc aagagagatg
     4861 caaacatt
//
LOCUS       DAZGCS010000214         4818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGCS010000214 DAZGCS010000000
VERSION     DAZGCS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.217"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..2354
                     /locus_tag="ACHK01_004896"
     CDS             93..2354
                     /locus_tag="ACHK01_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO8099684.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKLGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2496..3779)
                     /locus_tag="ACHK01_004897"
     CDS             complement(2496..3779)
                     /locus_tag="ACHK01_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO8099685.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3914..>4818)
                     /locus_tag="ACHK01_004898"
     CDS             complement(3914..>4818)
                     /locus_tag="ACHK01_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8099686.1"
                     /translation="SGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     TRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1168 a         1175 c         1231 g         1244 t
ORIGIN      
        1 cttgtcgcct tcctgcaact caaattattt agagtatatc catttattat ctttctgcgc
       61 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
      121 ttctcgccag taataacgaa ataattgctg aagaacattt caccggcgaa attaaaaaag
      181 aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat acgtccggaa
      241 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
      301 gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga
      361 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
      421 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
      481 tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca
      541 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggt ggtgagttgg
      601 taaaacagct gcttaatgac acctgggata tcgactatcc gggagttgtt gcggtgcatc
      661 tgaccggaaa accagcgccg tatgtggggc cgcaggatgt ggcgctggct atcattggcg
      721 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
      781 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
      841 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
      901 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
      961 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
     1021 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
     1081 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
     1141 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
     1201 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt
     1261 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
     1321 ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata
     1381 cgccaatcaa caacggatta agtattcgcc acaccacgcg taacttcccg aaccgcgaag
     1441 gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
     1501 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
     1561 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
     1621 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
     1681 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga
     1741 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
     1801 gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga
     1861 ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg
     1921 cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca
     1981 gcatggtata tgcggtgaaa ctaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc
     2041 agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt
     2101 ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag
     2161 tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt
     2221 ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa
     2281 gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa
     2341 accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat
     2401 gcgatgcttg cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccggataagg
     2461 cgtttacgcc gcatccggca aatagttaat agctcttact tcttcgcctc tgcaaccact
     2521 ttactaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
     2581 ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa cggacgatta
     2641 gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat ggcgctatca
     2701 cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc
     2761 acaccatcac cgaaagcatt gaaacggctg tttacggcga ggaaaccgta gtaaatgttg
     2821 gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt caccacgcgg
     2881 aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
     2941 atgtagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg gttctgcaca
     3001 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt
     3061 acctggtcac catcagtacg cagtgccacc gccggatggt tacctgcatc tacgctatcg
     3121 cccagcgtgt tttcgatggt cagattttgc agttgcaggc cattgttttg tgaccagaag
     3181 accgcagagc agagaacacc gatactgtcg ctgcgtttgc tctggcagct atcgtacata
     3241 taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
     3301 gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
     3361 gtaccgtaca gagtaattcc acccggagca gcagggacat acaccgttcc ctgatactca
     3421 ccaggcatca cggcaatata ctggcgcttg ttagtgcgct tgataattgc cgcatctacc
     3481 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
     3541 ggttgcgcag gcagggtaat cggggaagga ttccacgctg cagcacctgg tgtcagggat
     3601 gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa
     3661 gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gtggtgttga gctacaggcg
     3721 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
     3781 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
     3841 gttggcatta taaaaaaaca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3901 caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat
     3961 actgtgatgc catggtgtcc gacttatgtc cgagaagatg ttgagcaaac ttatcgctta
     4021 tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc
     4081 cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg
     4141 atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc
     4201 atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga
     4261 tggcaatttt tacgcctgtt ttgctttgtt cgacataaag atatccatct acgatatcag
     4321 accacttcat ttcgcataaa tcaccaactc gctgcccggt aacaacagcc agttccattg
     4381 caagtctgag ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag
     4441 ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg
     4501 ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta
     4561 acttggctga cgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt
     4621 ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc
     4681 tcatgtaatt tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc
     4741 gatcaagcca tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct
     4801 tgtgtttgtg tcctgaaa
//
LOCUS       DAZGCS010000215         4783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGCS010000215 DAZGCS010000000
VERSION     DAZGCS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.218"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..487
                     /locus_tag="ACHK01_004899"
     CDS             <1..487
                     /locus_tag="ACHK01_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8099687.1"
                     /translation="RTNVPGSDDLNGINVKYRYEFTDPLGLVTSFSYAGDKNRQLTRY
                     SDTRWHEDSVRNRWFSVMVGPSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGK
                     THDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYK
                     F"
     gene            552..3467
                     /locus_tag="ACHK01_004900"
     CDS             552..3467
                     /locus_tag="ACHK01_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8099688.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLATAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3467..4042
                     /locus_tag="ACHK01_004901"
     CDS             3467..4042
                     /locus_tag="ACHK01_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8099689.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSID
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(4140..4730)
                     /locus_tag="ACHK01_004902"
     CDS             complement(4140..4730)
                     /locus_tag="ACHK01_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8099690.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         1250 a         1191 c         1304 g         1038 t
ORIGIN      
        1 ccggacgaac gttcccggca gtgatgatct gaacgggatt aacgtgaaat accgttatga
       61 gtttacggac ccactggggc tggtgacgtc attcagctat gcaggagaca agaatcgcca
      121 gcttacccgt tacagcgata cccgctggca tgaagattcc gtgcgtaacc gctggttcag
      181 cgtgatggtg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
      241 tatggcttac agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa
      301 ggggaaaacg cacgacgtgc tgaccggaag tgatgacggt cgccacagca acacgtctct
      361 ggcgtgggga gctggcgtgc agtttaaccc gaccgaatcc gtggccattg atattgctta
      421 tgaaggctcc ggcagtggcg actggcgcac tgacggtttc atcgtgggtg tcggttataa
      481 gttctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc gggctttttt
      541 gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca ggaaaaccgg
      601 tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg gtggtgaaca
      661 cgctggcctc agaaaatccg gatgaagccg ggcgttacag catggacgtt gagtacgggc
      721 agtacagcgt tattctgttg gtggaaggat tcccgccgtc acatgccggg accatcaccg
      781 tgtatgaaga ttctcaaccc ggtacgctga atgattttct cggtgccatg acggaggatg
      841 atgcccgtcc ggaggcactg cgccgttttg aacagatggt ggaagaggtg gcgcgtaacg
      901 cgtccgtggt ggcacagaac acggcagccg cgaagaagtc agccagtgat gccggcacat
      961 ctgcccgtga ggcggcaacc catgcgactg atgctgcaga ttcagcacgt gcagccagca
     1021 cgtcagccgg acaggccgct tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat
     1081 caacaaaggc cactgaagcg gaaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg
     1141 cagctaccag tgccgctgcc gcgaaaacgt cagaaacgaa tgctgcagcg tcacaacaat
     1201 cagccgccac ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc acctcagccc
     1261 gggatgcgtc ggcttcaaaa gaggcggcaa aatcatcaga aacgaacgca tcctcgagcg
     1321 ccagtagcgc agcttcctcg gcaacggcgg cagcaaattc cgcgaaggcg gcaaaaacgt
     1381 ccgagacgaa cgccaggtct tctgagacgg cagcgggaca gagcgcctca gctgcggcag
     1441 gctcaaaaac agcggctgcg tcgtctgcca gtgccgcgtc aacaagtgcc gggcaggcct
     1501 cagccagtgc caccgctgcc ggaaaatcgg cagaaagcgc cgcatcgtcc gcttcaacag
     1561 ccacaacgaa ggctggcgaa gccactgaac aggccagcgc agcagcgagg tctgcttccg
     1621 cagcgaagac atccgagacg aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa
     1681 cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa
     1741 aagatgaggc gaccagacag gcgtcagcag caaagggcag cgccacgacg gcatccacga
     1801 aggcgacaga ggcagctggc agtgcgacgg cggcagctca gagcaaaagt acggcggaat
     1861 ccgcggcaac gcgcgccgag acagcggcaa aacgggcaga ggatattgca tccgccgtgg
     1921 cgcttgagga tgcgagcacg acgaaaaagg ggatagtaca gctcagcagt gcgactaaca
     1981 gcacttccga gtcactggcg gcaacgccaa aagccgttaa ggccgcgtat gagctggcta
     2041 acgggaaata caccgcacag gatgcaacga cagcacagaa agggatagtt cagctcagca
     2101 gcgcgaccaa cagcacatct gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct
     2161 atgaccttgc taacgggaaa tatactgctc aggacgctac gacagcacaa aaaggaattg
     2221 tccagctcag tagtgcaacc aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag
     2281 tgaaagcagc taatgataat gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta
     2341 aggcgctttc agcggatata acactgacgc cgaaagatat tggtacgctt aactcaacaa
     2401 caatgtcatt cagcggtggt gctggttggt tcaaattagc aacggtaacc atgccacagg
     2461 cgagttctgt tgtttcaatt acgttgattg gtggcgcggg atttaacgtg gggtcacctc
     2521 aacaggcagg tatatctgaa cttgttttgc gtgcaggtaa tggtaatccg aaggggatta
     2581 ctggtgcttt atggcagcgc acatcgacag ggtttacaaa ttttgcctgg gtcaatacat
     2641 ctggtgatac ttacgatatt tacgttgcaa tcggaaatta tgcgactggt gtaaatattc
     2701 aatgggatta taccagtaat gccagcgtga ctattcatac gtcaccagca tattctgcta
     2761 ataagccgga agggttaacg gacggtacag tttattcact ctatacacca tcagagcagt
     2821 tttatccgcc tggcgcacca atcccgtggc catcagatac cgttccgtct ggttatgccc
     2881 tgatgcaggg gcagactttt gacaaatccg cttacccgaa acttgcaacc gcttatccgt
     2941 caggcgtgat ccctgatatg cgtggctgga cgattaaggg caaacctgcc agtggtcggg
     3001 ccgtattgtc tcaggaacag gacggcatta aatcgcacac ccacagcgcc agtgcatcca
     3061 gtacggattt ggggacgaaa accacatcgt cgtttgatta cggcactaaa tccacgaata
     3121 acaccggggc gcatacgcac agtgtgagcg gtacagccgc aagtgccgga aaccatactc
     3181 atagtgtcac aggcgcatca gcagtcagcc agtggtcaca aaatgggtca gtacataagg
     3241 tagtgtctgc ggccagtgtg aatacaagtg ctgcaggagc gcacactcat agtgtcagcg
     3301 gcacagctgc atctgcaggt gctcacgcac atactgtcgg tattggtgct catacgcact
     3361 ctgttgcgat tggctcacat ggacacacca tcaccgttaa cgctgctggt aacgcggaaa
     3421 acactgtcaa aaacatcgca tttaactaca ttgtgaggct tgcataatgg cattcagaat
     3481 gagtgaacaa ccacggacca taaaaattta taatctgctg gcaggaacta atgaatttat
     3541 tggtgaaggt gacgcatata ttccgcctca tacaggtctg ccagcaaaca gtatcgatat
     3601 tgcaccgcca gatattccgg caggcttcgt ggctgttttc aacagtgatg aggcatcgtg
     3661 gcatctcgtt gaagatcatc ggggtaaaac ggtttatgac gtggcttccg gcgacgcgtt
     3721 atttatttct gaactcggtc cgttaccgga aaatgttacc tggttatcgc cgggagggga
     3781 atatcagaag tggaacggca cagcctgggt gaaggatacg gaagcagaaa aactgttccg
     3841 gatccgggag gcggaagaaa caaaaaacag cctgatgcag gtagccagtg agcatattgc
     3901 gccgcttcag gatgctgtgg atctggagat cgcaacggag gaagaaacct tgttgctgga
     3961 agcttggaaa aagtatcggg tgttgctgaa ccgtgttgat acatcaactg caccggatat
     4021 tgaatggcca gccctaccgt agggtaagat atataaattc tataattaga agtatctttc
     4081 catttaaggc taggaagggg ggcttggaaa acgtaaggaa tctcacaccg agattatttt
     4141 tatatatcag gtgtctgatt ttttgcttta gctcttaaaa tggtttgccg cgaggttttg
     4201 aattcttggg caatgtcact tatatttata cctgacttaa tttgctctag taccctctgt
     4261 atttgttcat catttaacac aggtgggcga ccaaatcgct ttcctgcgcc ccgtgctctt
     4321 aatatccctg aatgagtacg ctcaagtaaa aggtctcgct caaattcagc gactgctgaa
     4381 attacttgca tcatcatttt tccaggtgga ctggtcaggt caacgccccc caatgctaag
     4441 caatgcactt tgatacctgc tttggtcagt tgttccaccg ttttactgat atccattgga
     4501 ttacaaccga ggcgatccag ttttgtgaca atcaatgcat cacctctttt cagtcgatca
     4561 agcaactggt taaaaccagg acgctcactg gttgctgctg agccgctaat ttgttcttca
     4621 attatttgct gagatttgat gttaaaacct gcactttcga tttcccgacg ttgatttttg
     4681 gttgtctgtt ccagagttga tacccgacag taagcataaa ttcgagacat agtgagatct
     4741 tctatacgaa attggtgtac atatcataat gcatctcaga aaa
//
LOCUS       DAZGCS010000216         4743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGCS010000216 DAZGCS010000000
VERSION     DAZGCS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.219"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..420
                     /gene="grlA"
                     /locus_tag="ACHK01_004903"
     CDS             7..420
                     /gene="grlA"
                     /locus_tag="ACHK01_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO8099691.1"
                     /translation="MESKNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDI
                     AEAFGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIES
                     PVSTGPKRKNYRVGNGIVGQSNIWNEMIMRRKKEN"
     gene            complement(806..1261)
                     /gene="cesD"
                     /locus_tag="ACHK01_004904"
     CDS             complement(806..1261)
                     /gene="cesD"
                     /locus_tag="ACHK01_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO8099692.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1275..2813)
                     /gene="escC"
                     /locus_tag="ACHK01_004905"
     CDS             complement(1275..2813)
                     /gene="escC"
                     /locus_tag="ACHK01_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO8099693.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2813..3268)
                     /gene="sepD"
                     /locus_tag="ACHK01_004906"
     CDS             complement(2813..3268)
                     /gene="sepD"
                     /locus_tag="ACHK01_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO8099694.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(3274..3846)
                     /gene="escJ"
                     /locus_tag="ACHK01_004907"
     CDS             complement(3274..3846)
                     /gene="escJ"
                     /locus_tag="ACHK01_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO8099695.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3848..4225)
                     /gene="sctI"
                     /locus_tag="ACHK01_004908"
     CDS             complement(3848..4225)
                     /gene="sctI"
                     /locus_tag="ACHK01_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO8099696.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(4309..4605)
                     /locus_tag="ACHK01_004909"
     CDS             complement(4309..4605)
                     /locus_tag="ACHK01_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO8099697.1"
                     /translation="MDAANLSPSGRVLPLAATINGNNPVDETTGVMQPENGTNRTIRI
                     LGGVLISAGALAAIGAGIASLCVEDPSQKLGLGIAAGVLGGVTTLGSGLAMKYA"
BASE COUNT         1381 a          872 c          895 g         1595 t
ORIGIN      
        1 tggaaaatgg aatctaaaaa taaaaatggc gactatgtaa ttcctgactc agtaaagaat
       61 tacgatggtg aacctctgta tatcttggtt tctctttggt gtaaattgca ggagaaatgg
      121 atttctcgca atgatattgc cgaagcattc ggtataaacc tgaggagagc atcatttatt
      181 ataacttata tatcgagaag aaaagaaaaa atttcatttc gtgtcagata tgttagttat
      241 ggtaacttgc attataagcg ccttgagatt ttcatttatg atgttaacct cgaggctgtt
      301 ccgatagaaa gtccggtatc aaccggacca aaaagaaaaa actaccgagt cggtaatggt
      361 attgtggggc agtctaatat ctggaacgaa atgatcatga ggcggaaaaa ggagaattaa
      421 acgtattttt ttgagtatat tttatagaag aataaaaata aagctctatt caagggcttt
      481 gttttccagg ataagccttt ttctctgcca aagtttgaaa gtcatttcct gacaaataca
      541 ctctgggcta tagatgattg aaacagtcag atttctgatg ctaagttaag atatatactg
      601 cttattctat aagcggaaat gacattgagt acagcagaag atggttcagg cgcataaaat
      661 cctggttaat ggagtaaccc actcctgtag ataaatctgt cagcggtcgg tgctcaacgt
      721 tgggcgcact tcaccaaagc agctttatta tttccgatag aaaatcccca gacagcctgg
      781 ggattggtat ggcagatggt agtttttaat ctgtattacc taacaatttt ttaatatttt
      841 ctttatatgt agcgtatttt tcttgttcat tacaccacat taaacaagcc ttaaaggctt
      901 ttttggccat tttccgattt ccgaccaata agtagctaat cccagaataa taagatgccc
      961 tgggatcttg cataactaaa gttgcacagc gagcgaaaca cagttgtgca tcttcatgat
     1021 ttgataaacg ctgatgacac aaccccagag atagggtgta gtcataattc cagtgctcca
     1081 gatatgttag cagatgaaat agatttcttg cggttataac atcaccagat tgatatgctg
     1141 tataggcata agaatgaaga tcattgagat cttgttgcgt caaatttgtc agtgatgcta
     1201 acgtaccacc atcctggtag aaatcataga tatcctgtag agagctaaat ttcctgctca
     1261 taaagcctca tttattattc gctagatgct gattttatcg gggtcgcttt aatcaaaaag
     1321 agtcgaacaa cgctgtgttt ttgttttgtt gtactggaga ataagttacc aataacagga
     1381 atatcactta acaacggtat accatcttta cttgaactct ctttatcctg gataaatccg
     1441 ccaagcagca gactttctcc agcgcttaat gtcgcttctg ttgtcatttc tgaattttgt
     1501 acttcaggta agatgctgct ggcatcctga gcattacttt ggttagtcga ttgattgcca
     1561 tcctgaatat caagtaacaa acgaactcgt tcccgacgtt ttccggtaag actattcgag
     1621 ttgtcatcaa gtatacgtgg tgtcactctc agtaatgttc cgctggtaat actttccaga
     1681 gatgccacct tttctccgga aacttttgta taaaacgtta cgtttttatc aagaatcgcc
     1741 tgcatattat ttaaggttat aattgatggc tgagagagta tttttgcttt cgagttctgt
     1801 tgtaacgcat tcactctgat catgaaatta ttggcgttgc ttatcacaga agaactgaga
     1861 ttagcttgcg ccgttgagct gttaaaggca atagaacctt gccctgcatt taatgtaccg
     1921 gaccagttta ctcccagttg ctgaagatca tttgcatcaa catcaataat tgaaacagaa
     1981 atctcaattt gcctttgctc aatatcaagc tcagaaatta attgccgata tatgtccata
     2041 gttatttcac gatcttttac aacaactgca ttaagacgtg gatctgcgga aatctttaca
     2101 ctgttatcaa acaggttgcc gtttctttct acagcacctt tgcctgtgct gggaagtgag
     2161 ccattacttg ccatcgtctt caggatggag acgacgccag gcacgacaac attttggtcg
     2221 cggtatttat acgttatatc tgtcgctgat gcatagttta atttaaatac tctaacgaca
     2281 tctttatttt ttgctttaga ctgtgcttct ttatcaagac tttcagataa agatgtaatg
     2341 tattttatgc attcaggaac gccacgaact tcaatcgagt taaatgttgt gatttttctc
     2401 acgttacagg agtttttatt tactgagatt gtatcacgca gatattttaa cagtgagtca
     2461 atatccagat atgttggcgt aattatgctt cttgatattt catttgtttt ataaatatac
     2521 aaaatgttct catcataata ccatgtgaga tgatagagcc tggacagttt ttccagtact
     2581 ttcattggtt tttcattttt tatttcacca gagaaatcat cattcacaga attactgatg
     2641 aaaacaggga tgctataatt agcggcaaaa tcattgagga ttgcacgtac cggagagctt
     2701 tttgtaataa taaaatactc gttcttgcct aatctttttt ccagagagga cggggcagcc
     2761 tgagcactgc agcaaaatag tgctgtaaaa ataaaaaaac ttattttttt cattacacaa
     2821 ttcgtcctat atcagaaaac aaatgtgttg gaggcatcgt tgtatctgat gtagatgctt
     2881 gctctaactt atcaaattgc ttgtcaagca aagaggcgag tgtgtgttgc attattgctt
     2941 ttgacatgtt tgcgcgatga ttattatctt tattaaacag ttggcaaaga taccattttt
     3001 tattagattg aaaaatgccc atttgtttat attctttact aacggccaga atattacaga
     3061 gtctgatgaa ggtatctgtt ggaacctggc gaaaatcgcc attaagctcg atatacagaa
     3121 cccagtaact tccaaagtcg tgtaagaagg catttttttg atagatatga aaagtaagat
     3181 gctttggtga acctttgaca tcttttacaa aatcaagagc cgttagccag tcactatcat
     3241 tcttaacaat gccattatta ttgttcatac atattacccg tcctgtcctg aggatgactt
     3301 gataacgaca ctaatatttt ctagtttgag atcgtctacg ctattcttaa ctaagttttt
     3361 tatctgaatg actgatggtg ctaagttaac ctctggcgag ctaatgacta aaacagccgc
     3421 tgaggatggt tggctctcgt tattgttaac atttaatgaa acgctgcaat cgataacacc
     3481 ggggatttta cttagtaacc tttctatatc ttgttctttg agatagttga tttttgcatt
     3541 ttcttgtgat ggcgatgcca ctagctgact gggcggaaaa ataacttcaa tatctgcaaa
     3601 tttctttttc gggaagccat tgttatttaa aatggttatc gcacggacaa aatcctcttt
     3661 ttccacactt attgttacat ttccactttt gtccatttct ttagatacat tgacatcatt
     3721 ggataacaac aatgcttgca tctgattggc ttctttttcc gttaatcctg tatacaattg
     3781 ctctttacat gcaactgtca aacaaataaa aacaaataaa aaggtgtttt taatgtattt
     3841 tctcatttta ttgcattgaa accagttttg taaccgatgt tgaaagaaga cttgctaact
     3901 tggatacgac atcaacattg acgtttgcgt tgagcacatt cttttgtaat gtgagcacat
     3961 cttctggcga gagtccacct gcagataggg catcgaaact gagatcggtg ttcattgttg
     4021 caagatgtct ctttttaatt tcttcaatta aaacttgctc tggagaagat acaccagctg
     4081 ttttaggaag taattgatca agaaactgag ccgctccagc tgcagagggc gttggtgcta
     4141 acggggtatg gtcatcaacg aaatttgttt gggatacttt attaaaatga atattaatat
     4201 tttctgattg attaatattg ttcatattta cggttagtct cttttcatga gagaggtaat
     4261 ggcataatgc atccatgacc tctctatttt atttatgcga tgcgatgatt aggcatattt
     4321 catcgctaat ccgctgccaa gagttgttac gccacctaat acaccggccg cgattcctaa
     4381 gcctaatttt tgtgaaggat cttcaacaca cagtgatgcg ataccagcac cgatagcggc
     4441 tagagcacct gcacttatca aaactccacc taggattcta atggtacgat ttgttccatt
     4501 ttctggttgc attactcctg tcgtctcatc cactggatta tttccattga ttgtggctgc
     4561 cagtggtaat actctgccag aagggcttaa atttgctgca tccattaact tttctccatt
     4621 gtctaattta gacatttgtc tggttatcaa taaccaaatt atcccatggg caactattat
     4681 attatttaaa tagttgcaat gtacataaag tacatatact gtatatatta tccaacacgc
     4741 ttt
//
LOCUS       DAZGCS010000217         4663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGCS010000217 DAZGCS010000000
VERSION     DAZGCS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.220"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            340..4593
                     /gene="fdeC"
                     /locus_tag="ACHK01_004910"
     CDS             340..4593
                     /gene="fdeC"
                     /locus_tag="ACHK01_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO8099698.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1244 a         1074 c         1292 g         1053 t
ORIGIN      
        1 ttgccaatcg tggtaaatta aagacaggtc attattattc actctgaata atgaattatt
       61 cactgtccgc agagtaagaa atataactta ggtatctatt taatgacttg cacaaaaagc
      121 taaattttta cccataaata aaaatataat cccgcgccca accacctgat gagtggctac
      181 aggcactgga tatattaggt ggcggtgtac tttcttacat aaaggtattt ccttttctgc
      241 ggaaaaggaa accgggaaat ctccggtttt tctgacaagc agacgccatt atttgtgtct
      301 gcctatgttc gttaattcgt tcatcaggaa attatctcaa tgtcacgtta taaaacaggt
      361 cataaacaac cactatttcg ttattcagtt ctggcccgct gcgtggcgtg ggcaaatatc
      421 tctgttcagg ttctttttcc actcgctgtc acctttaccc cagtaatggc ggcacatgcg
      481 cagcatgcgg ttcagccacg gttgagcatg gaaaatacca cggtaactgc tgataataat
      541 gtggagaaaa atgtcgcgtc gtttgccgca aatgccggga catttttaag cagtcagcca
      601 gatagcgatg cgacacgtaa ttttattacc ggaatggcca ccgctaaagc taaccaggaa
      661 atacaggagt ggctcgggaa atatggtaca gcgcgcgtca aactgaatgt cgataaagat
      721 ttctcgctga aggattcttc gctggaaatg ctttatccga tttatgatac gccgacaaat
      781 atgttgttca ctcagggggc aatacatcgt acagacgatc gtactcagtc aaatattggt
      841 tttggctggc gtcatttttc aggaaatgac tggatggcgg gggtgaatac ttttatcgat
      901 catgatttat cccgtagtca tacccgcatt ggtgttggtg cggaatactg gcgcgattat
      961 ctgaaactga gcgccaatgg ttatattcgg gcttctggct ggaaaaaatc gccggatatt
     1021 gaggattatc aggaacgccc ggcgaatggt tgggatatcc gcgcagaggg ctatttacct
     1081 gcctggccgc agcttggcgc aagcctgatg tatgaacagt attatggcga tgaagtcggg
     1141 ctgtttggta aagataagcg ccagaaagac ccgcatgcta tttctgccga ggtgacctat
     1201 acgccagtgc ctcttctgac actgagcgcc gggcataagc agggcaagag cggtgagaat
     1261 gacactcgct ttggcctgga agttaattat cggattggcg aacctctgga gaaacaactc
     1321 gatacagaca gcattcgcga gcgtcgaatg ctggcaggca gccgctatga cctggttgag
     1381 cgtaataaca acatcgttct tgagtaccgc aaatctgaag tgatccgtat tgctctgcct
     1441 gagcgtattg aaggtaaggg cggtcagaca ctttccctgg ggcttgtggt cagcaaagca
     1501 actcacggac tgaaaaatgt gcagtgggaa gcgccgtcat tactggctga aggtggcaaa
     1561 attaccggtc agggtagtca gtggcaagta acgctcccgg cttatcgtcc aggcaaagac
     1621 aattattatg cgatttctgc ggttgcctac gataacaaag gcaatgcctc aaaacgcgtg
     1681 cagacagagg tggtcattac cggagcaggt atgagtgccg atcgcacggc gttaacgctt
     1741 gacggtcaga gccgtattca aatgcttgct aacggtaatg agcaaagacc gctggtgctg
     1801 tctctgcgcg acgccgaggg ccagccagtc acgggcatga aagatcagat caagactgaa
     1861 ctaactttca aaccggctgg aaatattgtg actcgtaccc tgaaggccac taaatcacag
     1921 gcaaagccaa cactgggtga gttcaccgaa actgaagcag gggtgtatca gtctgtcttt
     1981 actaccggaa cgcagtcagg tgaggcaacg attactgtta gtgttgatgg catgagcaaa
     2041 accgtcactg cagaactgcg ggccacgatg atggatgtgg caaactccac cctgagcgct
     2101 aacgagccgt caggtgacgt ggttgctgat ggtcagcaag cctacacgct gacgctgact
     2161 gcggtggata ctgatggtaa cccggtgacg ggagaggcca gccgcttgcg atttgttccg
     2221 caagacacta atggtgtcac cattggtaca atttcggaga taaaaccagg cgtttacagc
     2281 gctacggttt cttcgacccg tgccggaaac gttgttgtgc gtgctttcag cgagcagtat
     2341 cagctgggca cattacaaca aacgctgaag tttgttgccg ggccgcttga tgcagcacat
     2401 tcgtccatca ccctgaatcc tgataaaccg gtggttgggg gtacagttac ggcaatctgg
     2461 acggtaaaag atgcctatga caaccctgtg accagcctca cgccggaagc gccgtcatta
     2521 gcgggtgccg ctgctgtagg ttctacggca tcgggctgga caaataatgg tgatgggacg
     2581 tggactgcgc agattactct cggctctacg gcgggtgaat tagaagttat gccgaagcta
     2641 aatggacagg atgcggcagc aaatgcggca aaagtaaccg tggtggctga tgcgttatct
     2701 tcaaaccagt cgaaagtctc tgtcgcagaa gatcacgtaa aagccggcga aagtacaacc
     2761 gtgacgctta ttgcaaaaga tgcacatggc aacgctatca gtggtctttc gttgtcggca
     2821 agtttgacgg ggaccgcctc tgaaggggcg accgtttcca gttggaccga aaaaggtgac
     2881 ggttcctatg ttgctacgtt aactacaggc ggaaagacgg gcgagcttcg tgtcatgccg
     2941 ctattcaacg gccagccagc agccaccgaa gccgcgcagt tgacggtcat cgccggagag
     3001 atgtcatcag cgaactctac gcttgttgcg gacaataagg ctccgaccgt caaaacgacg
     3061 acggaactca ccttcaccgt gaaggatgcg tacgggaacc cggtcaccgg gatgaagcca
     3121 gatgcaccag tgtttagcgg tgccgccagc acggggacag agcgaccttc aacaggagac
     3181 tggacagaaa caagtaatgg ggtctacgtg gcgaccttga ctctgggatc tgccgcgggc
     3241 cagttgtctg tgatgccgcg agtgaacggc caaaatgccg ttgctcagcc actggtgctg
     3301 aatgttgctg gtgacgcatc taaggctgag attcgtgata tgacggtgaa ggttgataac
     3361 cagctggcta atggacaatc gactaaccag gtaaccctga ccgttgtgga cacctatggt
     3421 aacccgttgc agggacaaaa tgtgacgctg actctgccga aaggtgtgac cagcaagacg
     3481 gggaatacgg taacaaccga tgcggcaggt aaagccgaca ttgagctgat gtcaacggtt
     3541 gccggggaac acagcatcac ggcctcagtg aataatgctc agaagacggt tacggtgaaa
     3601 ttcaaggcgg atttcagtac cggtcaggcg agtctggagg ttgatagcgc cgcgccaaaa
     3661 gtggcaaacg gcaaagatgc ctttacgctg acggcgaccg ttgaggataa aaatggtaac
     3721 cctgttccag ggagcctggt gacctttaat ctgccccggg gtgtcaagcc gcttacaggc
     3781 gataatgtct gggtgaaagc caacggtgag gggaaagcag agttgcaggt ggtttcagtg
     3841 actgccggaa cgtatgagat cacggcatcg gcggggaata gccagccttc ggatacgcag
     3901 actataacgt ttgtagccga taaggctacc gcaaccgtct ccggtattga ggtgatgggc
     3961 aactatgcgc tggcggacgg caaagccaaa caaacgtata aagttacggt gactgatgcc
     4021 aataacaatt tggtgaaaga tagcgaagtg acgctgactg ccagcccggc ttcgttaaac
     4081 ctggaaccga atggcactgc gacaacgaat gagcaagggc aggctatttt caccgctacc
     4141 actactgttg cggcgacata cacactcaag gcgcaagtga gtcagaccaa cggtcaggta
     4201 tcaacgaaaa ctgccgaatc taaattcgtt gcggatgata aaaacgcggt actcaccgca
     4261 tcatctgata tgcaatctct ggtggcggat gggaaatcga ctgcgaagct ggaggtgaca
     4321 ctgatgtcgg caaacaaccc cgttggcggg aatatgtggg tcgacattca gacgccggaa
     4381 ggggtgacgg aaaaggatta tcagttcctg ccgtcgaaaa atgaccattt cgtgagcgga
     4441 aaaatcacgc gtaaatttag taccagcaag cctggtgtct atacgttcac atttaacgcc
     4501 ctgacctatg gcgggtacga aatgaagcca gtgacggtga ccattaccgc ggtggatgcc
     4561 gatacggcaa aggacgagga ggcgatgaaa taattttaag aatatgaaag taacattcta
     4621 ataaacaata atggcgttga catcttcaac gccattattt att
//
LOCUS       DAZGCS010000218         4620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGCS010000218 DAZGCS010000000
VERSION     DAZGCS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.221"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1312
                     /locus_tag="ACHK01_004911"
     CDS             <1..1312
                     /locus_tag="ACHK01_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8099699.1"
                     /translation="YNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYN
                     KYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIM
                     QPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEE
                     RFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSPNNTGITGIAGEHDQLNYGIY
                     VNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQ
                     LSDTFAILDASGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETEL
                     QGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQ
                     GSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1325..2283
                     /locus_tag="ACHK01_004912"
                     /pseudo
     CDS             1325..2283
                     /locus_tag="ACHK01_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2322..3221)
                     /gene="nhaR"
                     /locus_tag="ACHK01_004913"
     CDS             complement(2322..3221)
                     /gene="nhaR"
                     /locus_tag="ACHK01_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO8099700.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3287..4453)
                     /gene="nhaA"
                     /locus_tag="ACHK01_004914"
     CDS             complement(3287..4453)
                     /gene="nhaA"
                     /locus_tag="ACHK01_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO8099701.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1394 a         1057 c          967 g         1202 t
ORIGIN      
        1 ctataatgct ataactctgg gaaatggttg gaatacaccg ctgggtgcca tctcttttta
       61 cgctgcccga tcgagtagta agttaaataa tgataccaga cacgaaggta cgagctatca
      121 ggtcgcctat aataaatatc tagtacaaac agccactcga tttagcgtgg ccgcctggcg
      181 ttatgcttcg caggattaca ggacattcag cgaccatctt tacgaaaatg ataaacacta
      241 tcatcagagt gattatgatg atttttatga tattggcaga aaaaatagcc tttctgctaa
      301 tattatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcgc tttggcggaa
      361 ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca
      421 acacattagt tatactttct ctgccagcca atcttatgat gaaaataata aagaagagga
      481 gcgttttaat ctgtttatct ccattccttt ctactggggg gatgatattg ccaaaacacg
      541 tcaccaaatt aacttatcga attcgacctc attttccaaa gatggctatt cccccaacaa
      601 tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa
      661 tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga gaactcccat
      721 cgccataata gatggcagct atagtcattc taaaaacgcc tggcaaagtg gtggaagtat
      781 tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagt tatccgatac
      841 atttgcaatt ctggatgcgt ctgggttaga aggtgcacat attaatggac aaaaatacaa
      901 tcgaacaaac agcaaaggcc aggttgttta cgacctgata atacctcatc gtgaaaacca
      961 tctggtactt gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat
     1021 tattgcacct taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc
     1081 ctggtatata caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact
     1141 ggatctccag gaaaataata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt
     1201 agatgaaatt cccactggaa taaaagttgc tctcaatgat gaacagaact tattctgtac
     1261 tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt
     1321 tactatgaag tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc
     1381 tccctgtgca ttgaccaatg tcggtgaaca acgtggaaca tatatactca agccactgag
     1441 tatgaaaggt aatctaaccg ccgaaaaatt gaaaacaccg gaaatatcat tgagtgtaca
     1501 gacttgcaag agacaaccaa cgccattaaa gttcattttt taacacagaa cgctttaagg
     1561 tttaaagtcg gaagtcgtca ttatattatt aatttcccct ttgaatctga cggtaaaaaa
     1621 atagagcttc cagggaagtc atcttataat ttagaagaaa tgctatctgc gataaatttc
     1681 accattccat attctatcgc atctgtatca gatagttcta acaccgcaga catttttcct
     1741 ggaactcctt ttcctttgtt gttcgaaatt gaagtgagtt catgtacagg gaataatact
     1801 aattgtaata gcgtccgctt taattataat ttaaatacta ttttacaggt aaatttaatg
     1861 acctgtggtt ttgaagatca atccatcgac actggacgat ttgtactgtc tcgtatcaat
     1921 aatgaaaatt atcagtttca tcatataaga tttgactgca tccagggcga gcaaggtgaa
     1981 ttagtttttg aacccagtga tgttgaatat tactttgaac cagtgacgat acaggagtca
     2041 ggaaccagca tattaaaaaa tgagttagaa aacagtgagg acggtgcggg tcaaataggc
     2101 tttcaactct ccaatgatgg tacgaatgaa atccagtatg gtaaaagcaa ttactactct
     2161 tttcagcatc cccatgaagg aagtaatcag attcctctat ttattcgccc gcgtacatat
     2221 ggaaataatg tctcaagcgg gcaaataatg agccgagtca aaatcgtggt tatgtacaac
     2281 taatctatct cccccacctt aactttgggg ggaggtgccg attaacgcgc cgctggacta
     2341 aaaagcgcag aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc
     2401 tcagcaaaaa tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca
     2461 gttttatcgg cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc
     2521 atcgcaccaa aagctttcat caacgcggca tcatcaaact cgccgaggat ttctacgttt
     2581 aatccctggg agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga
     2641 atcaaaagtc gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta
     2701 caccagaaac tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta
     2761 gagtctatcg gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc
     2821 agcatttcgt gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg
     2881 gcgttaagta cgctactgac caggcgtttg gaaagtgcat cagccacacc aacgtcaaac
     2941 aataaattgg attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac
     3001 attttatcgg catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc
     3061 ttgcgtttaa ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg
     3121 gtttgtggag ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca
     3181 tgccagaagt aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat
     3241 aacaatgaaa agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac
     3301 gcaaacgaac gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga
     3361 taccgagttt cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa
     3421 taaagataga catagtaaaa ccgataccgc acaggatccc caccgccata atttgctgat
     3481 aagtcgttcc ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac
     3541 taatccccag cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg
     3601 tcaagccatc cagcgtgaca ccttgcagtg aaacgccagc attagcaaat gcaaacagcg
     3661 gcaaaatcag atacgccacc cacgggtgta acacatgctc cagtcgcttc gctggagaac
     3721 gcccatgctt ctctttcaaa ggaataaaga agccgacaat tacccccgcc agggttgcgt
     3781 gaacccccga tttcaacacc gcagtccaca acactacgcc aaccagaata tagacgcccg
     3841 tgcggcgtac accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacac
     3901 caagagaggc catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa
     3961 gatcgtcgat aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc
     4021 ccaacagcgc cagcacacca agtgcaaagg caatgtcagt agccgccggg atcgcccacc
     4081 cttcgcgggt aatcggatcg gcatagttaa aggccagata gagtaatgcc ggaacaatca
     4141 tcccaccaat agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt
     4201 gcatcagttc acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt
     4261 catttatcca taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg
     4321 gcgtctccag aaagtcgtga taccatccac tggttgcgcc gctattggcc ataatcatcg
     4381 ccaggatagc ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat
     4441 gcagatgttt cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc
     4501 cgcacaaatc atcgttaaaa tcagattata gtgtaggaat aactcgtttt ttacgatcca
     4561 ttgaagaatt tgcgccatca gcggtgagat gagtttttga cggctgtaag cgctcacgag
//
LOCUS       DAZGCS010000219         4567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGCS010000219 DAZGCS010000000
VERSION     DAZGCS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.222"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..571)
                     /locus_tag="ACHK01_004915"
     CDS             complement(38..571)
                     /locus_tag="ACHK01_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8099702.1"
                     /translation="MNAKIRYGLSAAVLALIGAGTSAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            697..960
                     /locus_tag="ACHK01_004916"
     CDS             697..960
                     /locus_tag="ACHK01_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099703.1"
                     /translation="MNEENTALFDRIFTLELQVGFLLPKIIKAMDALGKNNAVSDYLI
                     AEMEHLIKEIPKTDARDDKRFLRAAENALSTVKRSSGAQLIQE"
     gene            complement(1088..1423)
                     /locus_tag="ACHK01_004917"
                     /pseudo
     CDS             complement(1088..1423)
                     /locus_tag="ACHK01_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(1428..1643)
                     /locus_tag="ACHK01_004918"
     CDS             complement(1428..1643)
                     /locus_tag="ACHK01_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8099704.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDKVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1719..1988)
                     /locus_tag="ACHK01_004919"
     CDS             complement(1719..1988)
                     /locus_tag="ACHK01_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8099705.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPDGEMMM"
     gene            complement(2026..2208)
                     /locus_tag="ACHK01_004920"
     CDS             complement(2026..2208)
                     /locus_tag="ACHK01_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8099706.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            complement(2356..4293)
                     /locus_tag="ACHK01_004921"
     CDS             complement(2356..4293)
                     /locus_tag="ACHK01_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8099707.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPGTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTY
                     DRPDPRIKQLARRSTVTPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLAD
                     MVGQCAGGSADGVPWICGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     ANVPTNKPEEDPDIPASGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILL
                     HAGRTAELVGGEQVVMPPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWG
                     AEGGRALVVDDAGAAGGKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDG
                     TLTANQYALALYWPVSSLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNT
                     KLGSFGAFDNEWHTLGFRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTD
                     ITSGATYPVLIESITVEVNNP"
     gene            4270..4413
                     /locus_tag="ACHK01_004922"
                     /pseudo
     CDS             4270..4413
                     /locus_tag="ACHK01_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4459..>4567
                     /locus_tag="ACHK01_004923"
     CDS             4459..>4567
                     /locus_tag="ACHK01_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099708.1"
                     /translation="MAKHGAGCLPVNSVPAPESAIIPYTWLLIAPPHRGI"
BASE COUNT         1058 a         1309 c         1042 g         1158 t
ORIGIN      
        1 gcgttggcca acgtcatttt tcagcaaaat attctgctta tctgtcaatt ccccagcacg
       61 ccagtgcact ttcctggtct cgccgggata cctgaccgta gcaattattt gagcggatac
      121 ggcagtctct gccaccgtcc ttaatccacc agcgaatcgc ttcgcaggca ccttttcgat
      181 cacctgcatt aatccgtctg taaaacgtag acgggaaaca cttaccgggg ccaatgttat
      241 aaggacaaaa tgacgcgata cccgccttct ggggttcggt cagcggtact ttaatattgc
      301 gctccaccca ctccagcgcc ttatcacgtt caatggcgtt aacctggtcg cacttttcct
      361 tcgataactt catacccggg acgacaggct taccatccac tcgggtggca ccgcggcaga
      421 tggtccagat ccccgcacca tcacggtatg ccgtggtgtg gttaccttct ttttcgtcaa
      481 gaaactggtc gaggatttca ggcgcagacg tccctgcacc aatcagcgcc agaacggcag
      541 ccgacaggcc gtatctgatt tttgcgttca tggatattta tcaggattta tcggttccgg
      601 acccctggat atgttaagtc ttcagcccgc cggtggtggg cactggcgta aacccaacaa
      661 tgtgagatgg ctgcctcgca aagataatga acagatatga atgaagaaaa tacagcgctt
      721 tttgacagaa ttttcactct tgagttacag gtcggttttc ttctcccaaa gataatcaaa
      781 gccatggatg cacttggtaa gaataacgct gtgtcagatt acctgatcgc cgaaatggaa
      841 catctcatta aagaaatacc aaagaccgat gctcgtgatg ataaaagatt cctcagggct
      901 gctgaaaatg ctctttcaac agtgaagcgc agttcaggcg cgcagctaat tcaagaatag
      961 tttctttcat ttcatctgct gcaagcacct cattttttgt tggggtgctt tttttcaatt
     1021 cagtaacaca gcactccagt ttttcaatac gtgattcaac atcatctttt tctgaccgca
     1081 gtgttgacgg cggcatcttc agagaacaag taattcttcc cggtagcttt cctttgtagg
     1141 ttatcaacac atcctgcgcc tctaaaatta cggggcgatt ttccggcaac ggaccatccc
     1201 cttcacataa cccggcagca acatccatga aaaactgctt cgcctgcttt ttcgcctcag
     1261 cttcgtaaaa ctccagcgtg gcaccttcag tacggtcaag actaatcgcc acatctggca
     1321 acaacagtga cggataccca ccaatttcca gtgccacagt aacagtaatc ttatccgggt
     1381 aattatttat ccctttaaca accagttcgt attttttctt catcgcttta ctctccccgc
     1441 gccgccttac gacggtcctc tctgattttg aaatacaggt tagtcagata tgtcagcagc
     1501 ccaaacagca gactccccag cacgcctatt gccgcccact gagacggaga gactttgtcc
     1561 agcagctgca ggaaccagta gcccgttccc accgctgacg tggtgtatga cacacctgtt
     1621 gtgatttttt ccatctggta cataccccgt ctctcgcaat ccggaagctc acaacaacag
     1681 gaggggcatc agctcacacc gacagcccct gcgcatggtt acatcatcat ttcgccgtca
     1741 ggctgaggct cactgctacc gtcaggctga gacacgacgc catctgaaac agcactgtca
     1801 cccgcgccgt cttcaggctc aggagcagcc ggtcccccca gcagctcatc cagaatggca
     1861 tccacttcag catcaagacg cgcctcaaga ttctggcgga gttgctgttt cagtgcgctt
     1921 ctgacttctt cagagcgcag gacgtccttc actgcttcag cagtgaccag agattttatt
     1981 tctgacatgg gattttctcg ttgaaaggtg ttgttaagaa agttgctacg aaatgagagg
     2041 ctcttcgggt tttgttccgg ctgactggct ggcgctgatt ttttccgccg ccgcggcatc
     2101 aatctttttg cgtatatagt tccggataac cttatacccg ccacttacca gatataacgt
     2161 gcacaccacc gtgcagaaat acaataaaat aagctgtaca aatctcatta tccctcccgg
     2221 ttattgatat ggtgttgaca tcgttaatac ctgttggtta aaaaagtgtc ctgcatgttt
     2281 tgctttggat ataacgacat ttgccgccgg ttctggctcc ttgttttccc tgccccggcg
     2341 gccttttttt cctgcttacg ggttattcac ttccactgtt atactttcaa tcagcaccgg
     2401 atatgtcgca ccgctagtga tatcggtcac gcgcaatttg tctgccgtaa acgtgccgac
     2461 cggtgactgt gacagcatga acggcgtccc gtccttacca tcaatgaccg gcgtcacctc
     2521 aatactgttg ttaccggcaa aacggaagcc cagcgtatgc cattcgttat caaatgcgcc
     2581 gaatgacccc agcttcgtgt tctgaccagc atttcccttg tggtacatca cattaaggtc
     2641 tgtggcatcg ctctgtacgt aaaacgacgc cagcaggtta tgaccggcat taccttccag
     2701 tgtgacgccc tgaggcagtg aagaaaccgg ccagtacagc gccagtgcgt actgattagc
     2761 tgtcagtgtg ccatcaactt taaaacgaca actgataagc ccgcccttct ccagcaggtc
     2821 tgcgccatta ccggcatcat gctgcataaa ccacgaggaa cttcctgtct gtttggtcca
     2881 cctcagcgcc ttacctcctg cagcacctgc atcatcaact accaatgcac gccctccttc
     2941 agctccccat ccctgcggat tcagtaaacc acctgactct gttgcacggt aataaagcag
     3001 cgttgtcact gatttcccgt ccgttgacgg tgttgatggt gtgtccggtg acggcttctc
     3061 atccggcggc atcacaacct gttccccacc caccagttca gccgtccgtc ctgcatggag
     3121 aagaatcgct gaggcaagac ggtcagaaat aatccccctg cgtgcccatg agctgaaatg
     3181 gctcgcacgg tctgctgacg tccagtttgc cgacgtccgg gaggccgcac cgtaatatcc
     3241 tgatgccgga atatccgggt cttcttccgg tttgttcgtc gggacatttg ctccgttctc
     3301 atcggtcatg aacggcacaa agtgaatatt cttttccgtt ttgtttttgt aactgccgta
     3361 caccgtctgg taagtggatt cgctcttctg cttccagaaa taagttgtgt ctccacatat
     3421 ccagggaaca ccgtcagcag aaccaccagc gcactgtccc accatatctg caaggtccgt
     3481 acgatattgt tccactactt ctgtaaaacg ggctgtgtga tttgctggcg ttccgtcaaa
     3541 gtcaaattcc ccctgcatcc acaccacggc aagcagcaca tttttcgggt tctttgccag
     3601 cgcggctttt gtacgaccga tgagatcctt atacagcggc ctgcccacac cccagcgggt
     3661 agaactctct gaggcaccgg tcacgtcact gtatgttcca tctgccccgg tggtgaaagc
     3721 tgaaccacca cggcagcacg gaaccagaag aatacccgca ttcgccggta taaacggcag
     3781 cagctttttg gcaatatgca gcccctgccc cacggttccg tactgacctt tcgacaggtc
     3841 agctttcgga tggttaagac ggctcatgtc ctgtacatca tgcagacagt ggtctgccgg
     3901 aataatgtcg ttatatttac agggagcgcc atccggcgtc actgtgctgc gacgagccag
     3961 ttgctttata cgcgggtcag gacggtcata tgtctgcggc agaggaagcc cctcaccgta
     4021 agccatgccg ttcgactgcc cggccagtgg aataacgtag taataatctg gctccgtggt
     4081 gaccactccc ggatagccac catcccccgt gccgggcaca accactggcg tggtcacatc
     4141 cccctccgcg gcaatcgcct gcatcagggt ataaggggtt atggccaccg gactaccaaa
     4201 cggctgccag ccctctttca gtttgtgtgt cagcttttcc gcaagatctg acggcgacgc
     4261 cgccctgaca acatcataat gtttaatcga catcgaattt ctcccgtgta caggaacaga
     4321 gttaaaaagc cggaaccgga atcaaatcac aggatgacca tctgccagtg gctggtcgta
     4381 aaaaaaaggc cgcgccatgc gcagccggaa ataaagggat aacgatgata gtttgagaaa
     4441 aacagaaata acacttttgt ggcaaagcat ggtgccgggt gcctcccggt gaattcagta
     4501 ccagcacctg aatccgcgat tatcccatat acctggttgc tgattgcccc tccgcacagg
     4561 gggattc
//
LOCUS       DAZGCS010000220         4498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGCS010000220 DAZGCS010000000
VERSION     DAZGCS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.223"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(140..496)
                     /locus_tag="ACHK01_004924"
     CDS             complement(140..496)
                     /locus_tag="ACHK01_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8099709.1"
                     /translation="MRDMYEVMDRWGAWAASDNSGVDWQPIAAGFKGLLPHGKKSRIQ
                     CDDDEGIMIDSCVARLRRYKPEEYELIIAHFVIGISLRTIAKKRKCSDGTIRKELQTA
                     MGFVDGCLAMLAYSMA"
     gene            complement(514..1503)
                     /locus_tag="ACHK01_004925"
     CDS             complement(514..1503)
                     /locus_tag="ACHK01_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099710.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAATQPLADDPAVRTVFRNEAVIRRAGGVECHESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(1511..2308)
                     /locus_tag="ACHK01_004926"
     CDS             complement(1511..2308)
                     /locus_tag="ACHK01_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO8099711.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNMAPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSTPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2328..2717)
                     /locus_tag="ACHK01_004927"
     CDS             complement(2328..2717)
                     /locus_tag="ACHK01_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8099712.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            complement(2714..3040)
                     /locus_tag="ACHK01_004928"
     CDS             complement(2714..3040)
                     /locus_tag="ACHK01_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO8099713.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLVGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3037..3690)
                     /locus_tag="ACHK01_004929"
     CDS             complement(3037..3690)
                     /locus_tag="ACHK01_004929"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO8099714.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            complement(3690..4184)
                     /locus_tag="ACHK01_004930"
     CDS             complement(3690..4184)
                     /locus_tag="ACHK01_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8099715.1"
                     /translation="MSMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHYCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLQKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4181..>4498)
                     /locus_tag="ACHK01_004931"
     CDS             complement(4181..>4498)
                     /locus_tag="ACHK01_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HFO8099716.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRNHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSISTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
BASE COUNT         1031 a         1308 c         1064 g         1095 t
ORIGIN      
        1 tttgattgat aaagctaaaa aaaggatgag aactgttaaa tatctcgcat cagccatgtt
       61 ttatggaggc cttatttacg ttggatataa acagttccca gtcttttttg aaaaatcggc
      121 agtaaatcta ttttattttt tatgccatac tataagctaa cattgctaaa cagccgtcaa
      181 caaaccccat tgcagtttgc agttccttcc taattgtgcc atcagagcat tttctcttct
      241 tcgcaatagt gcgtaatgag ataccgataa caaagtgggc gatgatgagc tcatattcct
      301 ctggtttata ccttctcaac cgagccacac aactgtctat catgatgcct tcgtcatcat
      361 cacactgaat ccgtgacttt ttgccatgag gtaaaagtcc cttgaaacca gccgctatcg
      421 gctgccagtc cactccgcta ttgtctgaag cagcccaagc tccccaacga tccattactt
      481 catacatatc acgcatcaac tttctccaca aaatcaggcc agcacaccaa ttgccagcgc
      541 acgatcgata aaacgaaata tcaactccag ctgggagcca tacttctctt caaatgccac
      601 ggtatccgca tgcagctcgt cgtgatgctt tctgcacaaa ggcaacacaa agagatcatg
      661 cgcttttgta cccattcccc cctgaccgtg gcctatcaga tggtggggat catcagcagg
      721 ctttccacaa catgcacacg gctgcgtctt aacccagcgc gtgtactttt cattaaccca
      781 gcggcgacgt tttgggcgta acataaaaga ctccggcgac tccggatcca ctttcagcgc
      841 cagcaccttt ttcgccttat cctggatgat gctggtggca ggaaccgaag gcacaaggtc
      901 actttcccgg gtgacagatg gcacaacagg cttcggtaat ctcagtgcct tacgggctgc
      961 actttccggt aaggcatccg ccagatcatt acgaaccagc caccagcaca gttccggcat
     1021 tgtcacaacg tgactgtcat caaaaccgag atcccgacgc acgacagaca acacccagcg
     1081 ggcacagtta tccgttgcca ttgattccag ccgttccgtg aactggtcac gcagctggtt
     1141 atcgcagtgc cagcacagac gaattgctcc cggagcgtgc cgcattgtgg tcatgttctc
     1201 gctgtgccag tcagaatgag gccactgaca gcccttttca cgaagtaacc agctttcatg
     1261 acattccacg ccaccagcac gacggatcac tgcctcattg cggaacacgg tccgaacggc
     1321 aggatcatcc gccagcggtt gtgttgccgc cggaacggca ccactggcga aagatgaata
     1381 acgttccggc tctggctcca gcaggacacg cccctgcata aacaggggca tcagctctga
     1441 acctggcctg aacaatacga tccccatacg cggggcaatt tcaggggtca gtagtgctct
     1501 cacggtcacc tcaatgaacg gtatcgagta gctttaacag ctcagggaat cgggattcga
     1561 agaaatgcgg ctgcgtctcg cgcggatttg caggactggt gatgttcttg ccgaacatgc
     1621 agcctttcgc cgtcagcgac cagaattttt tgatgttgtt aatcgcggta cggctgtatc
     1681 gttcgcgttg ttcaacgatc cccagcttcg ccatctggtg atatgcctga ttagctgtca
     1741 ggcggatacc atactgcttc agcagtgcac tcagtgacag cgtggggcgg cttgagccat
     1801 caggcgcgtc agcaggagca tcaatggcat agcgcggtgc cagattcggt aagccaacag
     1861 cctcctggag tttctgacag gcaccaagca ctgaagagtt agacagattt aactcccggc
     1921 gcataaagtc cagcaggatc acgccagcct gcatcttgtc agcagcctgc ccggataatt
     1981 tttccggtgt gctggttacc atatcgaaag tacggatcac cttcagatgg aatgacgggc
     2041 tgatccacat tgcataggca tacaccagtt ctttgcagac atacgtccct tggttatttc
     2101 cgccacgaat aacgttaact ggcgccatat tgaccgagtt gcaaatttgc aactcgctta
     2161 ttaaacgttc ggtttgctca ttgcggagcc agaatgcagg cttatgttta tccagagaac
     2221 cggcagccct gtgcagatcg ttcaggctgt aacgcccata agcatcacga cgaacttcaa
     2281 taccatcaat aaccatcaga ttattcatac ttcgtttctc ctcttaatca ggcggctgca
     2341 cccgccggtt tctcgtactt actgatagtg atctcgacct tcccttccgg gataaccggt
     2401 ccccactcca ccagcattct tttcacctga ctgtcgtctt cccacacacc cgcgtgggtc
     2461 agggcgtcaa acagcgcctt gttatagttg tccagatcgc ggatccggtt atccggagga
     2521 aacaacacga tctccactga agcaggtgcc gacgttggtt ttggcagacg acgtaactgc
     2581 tcaactattg ctgcacacgc cgcgctctgg aatttgcgcc ccgccgcgct tatcaggctc
     2641 ttacctgcaa acgccccttt gttggggtgt cgccagtacg tgttcacgct gggcgggaaa
     2701 ggcaggatca gcttcatact ttcaggcccc tctcatgtaa ccagtgggct gcacgcagcc
     2761 tggcgttttc ctcaccgaca agcagtgagc ggataatccc gaccgcctcg ctgtcgtcgt
     2821 ccttcaccgc ggtatgaagc gtgatgcccc gggccacgcc acgctttatc gtgatgacgc
     2881 cttttttctc cagtgcgcga agatgttcta ccgctgcatt cactgaacgg tatcccagca
     2941 tggttgccac ctcctgattg gttggcggga agccacgttc tttctgataa gaaatcagca
     3001 tatccagcac ctgctgctgg cattgagtta acgtcgtcat gccaccatct ccctgaccag
     3061 tttttctgcc tgctggcgaa cctgcgccag aaaggcctca ccacatgcct caagttcatc
     3121 gcgcccgatg tagctgattg ccggtccctt ccaggtcttg tcgaaaacag caatagcacc
     3181 agcgaagaaa gcgcctgtcg gcacctgctt ctcatccttc gggataaacc aggcaggcag
     3241 ttcaaaaccg atacgcccgc gaataaaagc aatatgatct gcatcttccg gccaccacac
     3301 ttcgctggtg gcagctttga tcaggaaaac atagcgccca cctttatcac gcatggcact
     3361 ggcatgcttc atgatgtaac gcatgcctgt gatgtattgc ccctcatgct gactggcgcg
     3421 gctgtatggg ggattaccaa aggcagcacc tttaagctcc gcaagacgtt ctgaccagtc
     3481 atgcgccagc gcgttgtctt ccgccgtgta atacgcggca catttggcgt tatcaccgtc
     3541 agtgaacaga tccagaacaa acgggccaaa cagggtgtta attccccaga aaatgttatc
     3601 cggcgtgcgc cactgatcgc ccacttcctt cagttcatgg gctggtttgt tccgcagttc
     3661 caccagcgcc tggcaatatt tattactcat taagccccca cgtaattccc tgacagatac
     3721 cactcttcac ccgatgcagc gcgcttgctg cttttctgta agcactgctc acgacgcgcc
     3781 agaaaattgt ttcgttctgg ctgggagtgg ctttcacgga atgccgccat ccacaccgtt
     3841 gcagcacgac ggtataggcc cctggactcc agttcttcag cctggcgggt caggcacaaa
     3901 atcacccgtg gatcgttagt gccgacatag aaattgcgca caggtctggt ttcacgaact
     3961 ggttgcggtt ccggctcctg cgctctctca gtcaggcgcg ggaaatgtct gcgtgtatcc
     4021 ccttcacaat agtgagccac acgcccactc tgacgtaact tgcttgctga ctgcagaacg
     4081 cgctgccgtg agtaacctgc aaaagcatcc gcaatgtctc cggaagtaca gcccggatgg
     4141 gcttcaatga atttctgaac ttcattcaaa agactcatgc tcaccccctg aatcctgccg
     4201 ggatctggct gtagtccacg ttgtcgtaac tggatttgaa atatgggtct tcgcgttttt
     4261 ctgtgtatgt gctgatggac ggcgataagc gcagggaaag ctcatcccat ttttcccgca
     4321 gcttcgacgg gctgagcacg ttacggcacc agaacggatc gcgactgacg cggctgtaca
     4381 tctcgcagat ttgtttatga ttacgaccat cctgcacaca catcaggcga atttcgtttg
     4441 cccaggctgt ccagttcggt tctttgggac gaaccacctc gccgtcacat tcggcggc
//
LOCUS       DAZGCS010000221         4461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGCS010000221 DAZGCS010000000
VERSION     DAZGCS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.224"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..114
                     /locus_tag="ACHK01_004932"
     CDS             <1..114
                     /locus_tag="ACHK01_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO8099717.1"
                     /translation="TLLGERIMDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            136..852
                     /locus_tag="ACHK01_004933"
     CDS             136..852
                     /locus_tag="ACHK01_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8099718.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            885..1166
                     /locus_tag="ACHK01_004934"
     CDS             885..1166
                     /locus_tag="ACHK01_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8099719.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1163..1390
                     /locus_tag="ACHK01_004935"
     CDS             1163..1390
                     /locus_tag="ACHK01_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099720.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1383..1694
                     /locus_tag="ACHK01_004936"
     CDS             1383..1694
                     /locus_tag="ACHK01_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099721.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1822..2040
                     /locus_tag="ACHK01_004937"
     CDS             1822..2040
                     /locus_tag="ACHK01_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO8099722.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            2042..2599
                     /locus_tag="ACHK01_004938"
     CDS             2042..2599
                     /locus_tag="ACHK01_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO8099723.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            2890..3045
                     /locus_tag="ACHK01_004939"
     CDS             2890..3045
                     /locus_tag="ACHK01_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8099724.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            3165..3509
                     /locus_tag="ACHK01_004940"
     CDS             3165..3509
                     /locus_tag="ACHK01_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO8099725.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            3631..3903
                     /locus_tag="ACHK01_004941"
     CDS             3631..3903
                     /locus_tag="ACHK01_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099726.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMHQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            3905..>4461
                     /locus_tag="ACHK01_004942"
     CDS             3905..>4461
                     /locus_tag="ACHK01_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099727.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT         1183 a          962 c         1276 g         1040 t
ORIGIN      
        1 acgcttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg gtgggtgacg
       61 tttaactggg atagctggcg tccaaatgtc agcaatcaga gggttgtaaa gtaatttttg
      121 ttggaggacg ttttaatgga aactgtattt gacgcactga aagcactgaa aaaagcctct
      181 tcacaggtag tggcatcgcg ccttggaatc agccgcgaag atgctgtcaa cgaactgtgg
      241 aaactgaagc gccgtggtga agcggataac aagggttcga tgtggtggct gattcaggct
      301 ggtgaaagtg aaccagtgtc accggtaccg aaagtgacag cgcaaatgct gactgaggcg
      361 attgaacatc atggcccaca aacggcggat gagctggcac tgatgttcgg gattacctcc
      421 cgccgggcga attcatcact ggccatggca atcagcaaag ggcgtctgat tcgcgtgaat
      481 cagggcggta aatttcggta ctgcataccg ggcgctgatt taccggcaga gccgaaagcc
      541 gcatccatag cggaaacgga tggtaaagcc tttcctcagc cagcaggtgt tgcgttacca
      601 gtcggggaag cggaaacaca ggaagaaata aaaacggaaa gtgtggcggt cacagtgcag
      661 tcacagccgt cgttcaccag aaagcatccg gatggtctga ttttaccatc gctgcatgtg
      721 gctaaccgcg agctgcgccg ggcaaaaggt caggttcaga agtgggagcg agtctgcgcc
      781 gcgctgcggg agctgaacaa gtgccgggat attctccggg atattaccgc caccagagaa
      841 cagcagcggt gagtgggtgg aagacgtggt gccgggcgga aatcatgata ctccggcagt
      901 gtgcgggaac gaggaaggta aaaagcgttg gcgcacttat cggacgaact gaagcggcag
      961 tgagaacgaa ggcacgggag ctgggcatca gcatgatgtt acgtggtgat tttcacccgt
     1021 cggcaaaata ttctcagcgt gatattgagc tggcgcggca actgcatcag agaggcatgc
     1081 aaagaaggga aattgccaga aaattaggca tgccgctgcg catagtgaat aactacgttt
     1141 atttcgacag gagggtgtct gcgtgaaaat cctgtatcag gattacggcc cggtggggca
     1201 ggtggttatc agcagtactg taatggagtt tcggaagcat aaccgtgtgg tggatgctgt
     1261 gctgttaacc tgtccgggga tatcggcgag tcgtgcaggt gtgtttatta tgaagacgaa
     1321 attatatggc agtaaggcgt ggataaagaa ggcgtatcgt gtagcgttgc aggaggttaa
     1381 cagtgagtaa aattaaagaa atgccggtag ttcgtgacgg atatggctac tggacacatc
     1441 ctgaatatga aaaattctgt gatggtcggg aatatatttc aacggaagag tttaacgcct
     1501 ggatggagga aaataatctt caatacgtcc tctgcttcag agatgaagga tgtgctgacc
     1561 ttgatgcgtg tgatgctgat atttctgcat gggaaccgga acgaccagag ggcgatggct
     1621 ggtttattgg ttccattcat gatacggaag atggcccggt ttgtgtctgg ttgcgaaata
     1681 aggctgaagc ataaaggcga taaaccaact aacaactaaa tactgaagat ttaaatcaga
     1741 aacgattttt attaaatcct taaccggagg gattcctgca ccctcagaac atcaggaggc
     1801 cgcccgaaag ggcggtaaga aatgaaacat tatttagaaa aaaattaccc acgaaagagc
     1861 agaacaacag agtttctgtt tttcattctg tttatagtgt tgatgatacc gatatccccg
     1921 ctattactgg tctggataat tggaaggaca tttgaaccag ttattgagct atataccgat
     1981 gtgacatggg aatcattcag cgcactgcac aataaaatta atccgtataa ggaaaactga
     2041 tatgagcact attaccagag aacgcgcgga gattaaatca tacatcacag gcttcctgag
     2101 cgactcggcg cacgataaca agtcttcaga cagcctgctg gctaatgtgt ttcgtatcgc
     2161 gctggcatca ctggaagcag agccgatagc aatggtagtg cctgatgaaa tggatttgct
     2221 tacctgccat ctcgacggtg taactaaaac atatgctgat ggctggaacg cctgccgcgt
     2281 cgccatgctt caggccggaa actttcggga aaataagaat tcgtcaacca acaattttcg
     2341 ggaaatctcg gaaacgtcaa ccagatctcc gataactctg gatggctgga taagctgtac
     2401 tgagcgaatg cctgaaaaga gccagaacgt gcttatttcg atgaatatcg atagcgaggc
     2461 tgggccatta atatattccg cacgctatct cggaggcacg ttccggcgcg gaggtatagc
     2521 agttagtccg ggtaatgatc ttaggcaagc aacccactgg atgtcgctac cagaaccgcc
     2581 gcaggaggtg aatcaatgac ctggcctgaa gcattcacaa cggtaggaat tgcgatggcg
     2641 gtggcgctgg tggtgtattc gatttgccgc tggggataaa aacggtttgc gggaaaagga
     2701 tagttaagta gaattgcagc gggtgcttga ggctatctgc ctcgggcatg aacaccaacg
     2761 gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct
     2821 gaggctcaat ccatgctgaa cacatgtagg ttagcctctt acgtgccgaa aggcaaggag
     2881 aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc
     2941 gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtgcgaat ccgaaccggt
     3001 cagacggagg tcgctgtctt cgtagactac gaatctgaga agtaagagac caggcggggg
     3061 agtaatctcc cgccacctct gatgtgtcag gcatcctcaa cgcacccgcg ctttaccata
     3121 ctgaaaatgc tgtttgaatg ttcatctctg aaagaggact atgaatgaaa aaggtattga
     3181 ttgcagcact tatttccggt gtgtcttttg gcgcttttgc acagcagggt ggtttccagg
     3241 ggccagaagc agagcgttca acagtagcgc aggcaaaaga actgaaggat gatgcatggg
     3301 ttatccttga agggagcatc gttaaaaaag tgggtgatga acgttatgag tttcgtgaca
     3361 atagcgggac aattgtcacg gatattgatg acagcgtatg ggccgggcag aatgtttctc
     3421 cgaaagacaa agtaagaatt gagggtgaaa ttgataaaga cctgagcagt gttgaagttg
     3481 atgtaaaggc actgaaatta ttaaagtaac cgcccctgct tgttaagccc gtcttactga
     3541 cgggttttct gtttgtacat tccggcgtat tgccttacaa ttcgcgcagt cagcctgaac
     3601 aactgacacc tgctgtcacc ggagaatccg atgacacaac acataaaatc ccacaattct
     3661 gaagccgacc cggaaattaa gcaggggagg cgttttcgtg cgcctcagta tggctggttt
     3721 cactatctgt tctgtacgat cgatgaggca gatatgcatc aagaggcgta tctgcgtcgc
     3781 ggtgtccgtg tggagcggag tctgaacgct gatcgtctga cctggaccgt ttctgtatat
     3841 cttcctgttc gtgcacatct gccacggaca catgcctgct accgtcagcg cgtctggagg
     3901 taatgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg tggtccttaa
     3961 gcccggtcgt gaatcattgc cagtttttca tcgcggcagg gtgctggtgg agccggaacc
     4021 gaaaaacatg cgggcgctgc catctggagc ggttcctgct gttcgccagc cgctggcgga
     4081 agataaatca ctgctgccat ttttcagcga tgagcgggtg attcgtgcag ctggcggcgc
     4141 tggtgcactg tctgactggt tattacgtca cgtgaaatcc tgccagtggc cacacggcga
     4201 ttatcatcac agcgaaaccg ttattcacag ttacggtgct ggcgcaatgg tgttgtgctg
     4261 gcactgcgac aaccagctgc gcgaccagac ctccgaatca cttgagcaac ttactcaaca
     4321 aaatctgaca gcctggatga ttgacgtcat acgccatgta atgaatggca cgcaggagcg
     4381 ggaattatcg ctggctgaat tatcctggtg ggcagtctgc aatcaggtgg tggacgcatt
     4441 acctgaggca gtatcgcgtc g
//
LOCUS       DAZGCS010000222         4390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGCS010000222 DAZGCS010000000
VERSION     DAZGCS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.225"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHK01_004943"
     CDS             complement(<1..209)
                     /locus_tag="ACHK01_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8099728.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHK01_004944"
     tRNA            complement(324..399)
                     /locus_tag="ACHK01_004944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHK01_004945"
     tRNA            complement(406..480)
                     /locus_tag="ACHK01_004945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHK01_004946"
     tRNA            complement(597..681)
                     /locus_tag="ACHK01_004946"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHK01_004947"
     tRNA            complement(690..765)
                     /locus_tag="ACHK01_004947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK01_004948"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK01_004948"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO8099729.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK01_004949"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK01_004949"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO8099730.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK01_004950"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK01_004950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO8099731.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1123 a         1103 c          984 g         1180 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt
//
LOCUS       DAZGCS010000223         4384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGCS010000223 DAZGCS010000000
VERSION     DAZGCS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.226"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..130)
                     /locus_tag="ACHK01_004951"
     CDS             complement(<1..130)
                     /locus_tag="ACHK01_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8099732.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMG"
     gene            1362..2846
                     /locus_tag="ACHK01_004952"
     CDS             1362..2846
                     /locus_tag="ACHK01_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8099733.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(3168..3770)
                     /locus_tag="ACHK01_004953"
     CDS             complement(3168..3770)
                     /locus_tag="ACHK01_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099734.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(3857..4063)
                     /locus_tag="ACHK01_004954"
     CDS             complement(3857..4063)
                     /locus_tag="ACHK01_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099735.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT         1128 a          895 c         1125 g         1236 t
ORIGIN      
        1 tacccataat gccaatcacg ggttcgtgac tggtgagttt gcgcagatgt tccaggatgt
       61 gacgggaaag tgagtaaggc agggaggaga gcggtttttc aattgcctca atggcatcag
      121 acggattcat acatgtattc ccggtgaaaa aagacaaaac cccgcgcacc ggaaaggtgg
      181 cgggggattc acggagataa tatctgtctg aaattgtttt gaatcgatgg gattgttgcg
      241 tattaacatg aaggagatca ggacttatcc gttactgggt gatctccttc catgaaaata
      301 cgccgttatt gttaaacagc aggctgattt ctccgtaaag gggaaaatat ttatcggctg
      361 tatgattatc aggtgtgtct gtgtttactg ctgtgagtat tttttagtgt attgtggggg
      421 agatgtcagt gggagatatt aaatttacgt ttataattat cttacttctt ctgtgtgttt
      481 tttataagag tgcacacgta atgatatctg ttgataccat gtggttaagt gtttcccttc
      541 ctggtcatgc gtaatgatta aatgcttttg tgacaccatt cttccggtct gaatatttat
      601 cccgttcttt tttatttgtg cagtgatact gtcggaagag aattcagtaa tgtgaggtgt
      661 cctggtcagg ataatatctg ctgagatatt attagttgag tgtgttttta ctgagcaaaa
      721 gctggctttt aattcctgta ctgaaacact gtcgctcgtc cggggttaac tgaaactgga
      781 tgatgatgta aaatgaaggc tgtttttgtg tttgtcgaag aagtgttatt ttaatccatt
      841 ctcatcattg gctggcgatt atacagaagt atttgaagaa aaaatggtgc attttatctg
      901 tgtcagcagg gaagaagtat tttattgatg tatcagtatt aaatggtttt tgttatttat
      961 aaaaatgtca tcctgtccat agaatattgt actcagcatt cagtgcagga actgtagtct
     1021 tctttatttt attcttcttc aggagggggg gtgattcctg aaggcgttat tcccggtgtt
     1081 ctgtttatga ttcttcccgg caggaaaccg gtatctgcaa tacccgggat gatgcttttt
     1141 gttcgtacgt ggttgtgtaa aatttgaata gtggatgtgc tccgaaggga aggaggggac
     1201 acacagattc attttgtctt tcttcagcag caaaatacac tgtggttatt atttaaccat
     1261 taaatcaaca aaaattttta cctcatattg atttgactgc tatttttgct attaatagcc
     1321 agaattgcag tgcttttata tctgtgtgta gtgaggttac tatgtcattc ggtcagcctt
     1381 gtgatgagtt tcccctgagt tcgctgcctc cactcattcg tgatgccgtt attgaagctc
     1441 agcaaattac gcaggctccc cttgggcttg tggcagcgtc tgccctgggc gctgtttcac
     1501 ttgtctgcca gaatctgatt gatgtctgca gactgaatac actgcggggg ccggtctctc
     1561 tttttttcct gacgcttgcc gagtccggag aacgaaaaac cgcagtggac aaattattaa
     1621 tgaagcccct gtaccagcag gagatgcagt tatattccag atacaaaagt gaactggctg
     1681 tctggaaaaa caaggaagag cttttaaagg ctcagaaaaa agcattgttg tcaaaactga
     1741 ataaagaact ccgcaaaggc gcggatgagt cagaaaccct cagacaactg gaagtacttc
     1801 agaaaaacag tgcggaagaa cctgtgaggt ataaattcat ttttaatgat gccacgactg
     1861 cggcaattaa gaatcagctt tgtggtaaat ggcgttcagt gggtattatg tcagatgaag
     1921 ccggtattat atttgatgga tataccctgt ctgagttgcc ttttattaac aaaatgtggg
     1981 atggctctgt attgtctgtg gacagaaaga atgaacccga acagatgatt gaaaatgcca
     2041 gaatgacgtt atccctcatg gtgcaacccg gtctttttga ccgatatatg gagcgaaaag
     2101 gttctgttgc cagggacagt ggttttcttg cccgttgtct tatcagtaaa cctgcaacca
     2161 cgcaggggaa acgctttatt aacggagctg tgattccggg aggatcatta acggcatttc
     2221 atgaacgatt aatggaactg gccagaggca gtattgaaaa atccagtgag gatgaacggt
     2281 attgtctgca tttttcgcct gaagcgcaaa aaatatttat tgagcactat aatgttctgg
     2341 agcaggattt atccccttcc ggacccttaa gcccgtttcg gggacatgtt tcaaaaaaaa
     2401 cagaaaacat tgcaagaatt gcagcgttat ttcagtattt cagctatggt gaagggaaaa
     2461 tctctgccga tatcatgacg tctgctgtcg taataagttc gtggtacaca gatgaatata
     2521 aaaaattatt tgcgttacct gatgaatctg aactgcagca gaaggacgcc gaggagctgt
     2581 tcgactggct cattgaggag tgccgcggag aatgcccgcc ccgtgtcagg aaaaattata
     2641 ttctccagtg tggcccgggc cggttcagaa acaggaaaaa actgaacgca ttgctgaaca
     2701 ttcttgaatc tcagttcagg ctttctgtcg tgccggaagg aaaaaccatg tatgttctgc
     2761 ttccccagat agccagttta aagttgtctg atgtctctgg tatttttacc tcgggttatc
     2821 actacaataa gctgagagcg aagtaaccgg tgagcagttt ttctccatta tgcttcagac
     2881 ttagatgatt gattattatg gggatttcat caccgatatt cagatgtatg aatatcggtg
     2941 atgaactaaa gctcaggagc tgcgtggtgg tgatgaatca acaggcatat aactgatacc
     3001 cgccggtcag aagttccagc ctgcgacttt cctgtctgcg tgcccgcagt ccggcgttca
     3061 ggtgcagcgt gtgacagaac gcacggagag cgaaacggca ggcagacagg ccgttcagtc
     3121 agccctggca ccctgcccgg ataccagctt ccctgaaaca actggcatca ggtgcgctta
     3181 tcgtcaaccg ttgtgcggta tgccaggagc acgtactgat ccatcagatc cgtcagtgct
     3241 gcgctctgct tctgacggtt gatgatccat tcctccagct ctttttctga tttccgtttc
     3301 agattctccg tccagtcgtc aaggcagttt ccggtcatta aatcaagcca gcaaagccag
     3361 accagtttga ggcgcaactc cgtgccacca aactgacgga agaggctgat cagtttattt
     3421 gccagcgaac gctgcctcag acgttcacat tcagacagga gaatgccttc cagcatcgga
     3481 tgatgaacca gcagatgttc actggtgcat aaattcacta tcagatcatc atccaggggc
     3541 ggcgtggtcc gtttgtccgg accataaatc agccggtgca gactctgggg tacgatggca
     3601 atctccggtt tttcaccatt gaatgacacc cacccgggcg tcagattatg gaagtagtag
     3661 atattcggat gacacacgcc ccaggatacg gcgtgccggg tgaagccggt cagccatttc
     3721 tggtagcttt cgtggagcag ctcgtaacgg gtctgatact gtggtttcat tttcatattc
     3781 ctcacggggg ataacaggca gcagaacgcc gccggcaggc cggagactgc gtctgctgca
     3841 gatgaacggt taagaattac ggacgggact gtgcaatacg cgcctgcagc caggcttcca
     3901 cttcgctttt cagccatcgg gagctgcggc ccattttgat gggcgcagga aagtcaccat
     3961 ccttgatgag tttgtaaaac cacttatcgg tcaggccggt cagttgagtg ataaatgcca
     4021 tgtcgaccat ctggtcatcc atcagtgaaa ctggggtgat catgagaatg ttcctccgga
     4081 tgttgttaaa ccagccggtg tggtcatgaa aacaacgcac caccttcacc ggcataacaa
     4141 ccggggagaa acgtaaaaaa taatcgggaa tgtgaccgcc aggagataaa cggggaggtt
     4201 acggtggctg agccgtaccg gtatgccggg tggttgttac gaaacgatgc ttccgcacgc
     4261 gtgcgcgata aagaagggat gattttcgtg aggaagaggg gaaaactggc gttctgataa
     4321 ttgcagagtt atttacgggg cggttacgat ggggcagacc gtgtccggtg ggcaggcgac
     4381 aggt
//
LOCUS       DAZGCS010000224         4375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGCS010000224 DAZGCS010000000
VERSION     DAZGCS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.227"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..729
                     /locus_tag="ACHK01_004955"
     CDS             <1..729
                     /locus_tag="ACHK01_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO8099736.1"
                     /translation="AVALPENFRIHDLEKFNLNRFRFRGALSTASIDDFTRYSKDLAD
                     EGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGERNSQKS
                     LAEWIEDWADYLVGFDANGDAIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLMES
                     VEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQEEMAN
                     EFRDLLVEKFKDSKVETFIGTFTA"
     gene            857..1393
                     /gene="yfdR"
                     /locus_tag="ACHK01_004956"
     CDS             857..1393
                     /gene="yfdR"
                     /locus_tag="ACHK01_004956"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8099737.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            1384..1746
                     /locus_tag="ACHK01_004957"
     CDS             1384..1746
                     /locus_tag="ACHK01_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099738.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            1746..2051
                     /gene="yfdT"
                     /locus_tag="ACHK01_004958"
     CDS             1746..2051
                     /gene="yfdT"
                     /locus_tag="ACHK01_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFO8099739.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1967..2146
                     /locus_tag="ACHK01_004959"
                     /pseudo
     CDS             1967..2146
                     /locus_tag="ACHK01_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2278..3441
                     /gene="intD"
                     /locus_tag="ACHK01_004960"
     CDS             2278..3441
                     /gene="intD"
                     /locus_tag="ACHK01_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO8099740.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(3457..3533)
                     /locus_tag="ACHK01_004961"
     tRNA            complement(3457..3533)
                     /locus_tag="ACHK01_004961"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3497..3499),aa:Arg,seq:tct)
     gene            3776..>4375
                     /gene="fimZ"
                     /locus_tag="ACHK01_004962"
     CDS             3776..>4375
                     /gene="fimZ"
                     /locus_tag="ACHK01_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO8099741.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL"
BASE COUNT         1298 a          951 c         1044 g         1082 t
ORIGIN      
        1 gctgtggcat tgcctgagaa tttccgcatt catgatctgg aaaaatttaa tttaaatcgc
       61 ttccgtttcc gtggtgcact ttccactgcc agcatcgatg attttacccg ttattctaaa
      121 gatcttgcag atgaaggcac ccgctgcttt atcgatgccg ataatatgcg tgccgtcagt
      181 gtgcttaacc tgggtactat tgatgagcca ggtcacgcag ataacactgc cactctcaaa
      241 ctgaaaaaga cagcaccgtt ctctgctctg ttgtctgtta acggcgagcg taactcccag
      301 aagtcactgg cagaatggat cgaagactgg gccgactacc ttgtgggctt tgatgctaat
      361 ggtgacgcca ttcaagcaac aaaagcggct gcggcagtcc gtaaaatcac gattgaagca
      421 aaccagaccg ctgattttga agataatgac ttcagcggca aacgctccct gatggagtct
      481 gtcgaagcga agaccaaaga cattatgcca gtggcatttg aatttaaatg cgttccgttt
      541 gaaggtctga aagaacgtcc gtttaaatta cgcctcagca ttatcactgg cgatcgtcct
      601 gtactggttc tgcgcattat tcagctggaa gcggtgcagg aagaaatggc taacgaattt
      661 cgtgatctgc ttgttgagaa attcaaagac agcaaagtag aaacctttat tggtactttc
      721 accgcctgat ttcattactg caaatgcccc tgcgggggca tttatggaaa cgtaattaac
      781 tcaataatca ccggatggtg agggcttcct tttaccagaa ttcagcgcgg tgcagtgcat
      841 atacgtggag aacaaaatgt catttattaa aactttttcc gggaagcatt tttattatga
      901 caagataaat aaagacgaca tcgatattaa cgatatcgcg gtttcccttt caaatatctg
      961 tcgctttgcc ggtcatcttt cgcacttcta cagcgtcgcc caacatgcgg ttctttgcag
     1021 ccagctggtg ccgcaggaat ttgcttttga agcgttaatg catgatgcaa cagaagcgta
     1081 ttgccaggac attcccgcac cactgaaacg ccttcttcct gactataaac ggatggaaga
     1141 aaaaatagac gccgtaatcc gtgagaaata cgggttaccc ccagttatga gtacacccgt
     1201 gaaatatgcc gatctcatca tgctggcaac cgaacgccgc gatctcgggc ttgatgatgg
     1261 ctctttctgg cctgtactgg aaggcatccc ggcaacagaa atgttcaacg tgattccact
     1321 ggcaccgggt catgcctacg ggatgtttat ggaacgtttt aacgatttat cggagttacg
     1381 caaatgcgca tgaatgtttt cgaaatggaa gggtttcttc gcgggaaatg tgtaccgcga
     1441 gatctgaaag tgaacgaaac aaatgctgag tacctgttac gtaaattcga cgcgcttgaa
     1501 gctaaatgtg cggcactgga aaacaaaata ataccagtgt cagctgaact gccaccagca
     1561 aatgaaagtg ttctgttatt tgatgctaat ggagaaggct ggctgattgg ctggcgttct
     1621 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
     1681 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
     1741 gcataatgac cacatttacc aataaagaac tgattaaaga aatcaaagaa cgaatcagca
     1801 gcctagaggt tcgagacgat attgagcgcc gtgcttatga aatcgcactc gtatctctgg
     1861 aagtagagcc agatgaacgc gaagcctatg aattattcat ggaaaagcgt ttcggtgact
     1921 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
     1981 tcggttggat cgtctggcag caacgagctg gtatccattt ttcaacaatg tcacagcaag
     2041 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaaaat
     2101 atccagaccg actgccatta actggatacg cacaggccgc ctacaggcaa cacgtaaaga
     2161 tccaaccaag ccaaaatctc cttacctcac aacacggcaa gcttgcattg cggcgcttca
     2221 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     2281 tcactcttcc gcagaaatga aatatggtat gcctcgtatt cgctcccggg cgggaaacga
     2341 attaaggaat ctcttggcac aaaggacaag cggcaagctc aggagttgca cgacaagcga
     2401 aaagcagaac tctggcgagt agaaaagcta ggggatttac ctgatgtcac ttttgaagag
     2461 gcctgcctaa gatggcttga ggaaaaagct gataaaaaat ctctcgattc agataaaagc
     2521 cggattgagt tctggcttga acattttgag ggtataaggc ttaaagatat ctcggaggca
     2581 aagatttact ctgctgtaag cagaatgcat aacagaaaga cgaaagaaat atggaaacag
     2641 aaagttcagg ccgccatcag gaaaggtaaa gaaccgcctg tttatgaacc aaagccagta
     2701 tcaactcaga caaaggcaaa gcatcttgcc atgataaagg ccattctccg tgctgcagaa
     2761 cgcgactgga agtggctgga aaaagcgcct gtcatcaaga taccagcggt cagaaacaag
     2821 cgagtcagat ggctggaaaa ggaggaagca aaacgcctta ttgatgagtg ccccgaacca
     2881 ctgaaatctg tcgtcaagtt tgcgctggca actggtctga gaaagtcgaa catcataaat
     2941 ctggaatggc aacaaatcga catgcagcga cgagttgcct gggtgaatcc agaagagagc
     3001 aaatcaaacc gcgccattgg tgtggcgctg aacgataccg cctgtaaagt gttgcgtgat
     3061 caaataggca agcatcacaa atgggtgttt gtacatacca aggcggctaa gcgagcagat
     3121 ggaacatcaa cgcctgcggt caggaagatg cgcatcgaca gcaagacatc atggctatca
     3181 gcttgtcgtc gtgcaggaat tgaagatttc cgtttccatg acctcagaca cacctgggca
     3241 agctggctga tccagtcagg cgtcccatta tctgtgcttc aggaaatggg cggatgggag
     3301 tccatagaaa tggttcgtag gtatgctcac cttgcgccta atcatttgac agagcatgca
     3361 aggaaaatag acgacatttt tggtgataat gtcccaaata tgtcccactg tggaattatg
     3421 gaggatataa agaaggcgta actgattgaa ttgtaatggc gcgccctgca ggattcgaac
     3481 ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg cgcgttgata
     3541 ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc ttgctgagtc aatggctttt
     3601 gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt tcgctatagc
     3661 ggcaaacaaa cacacaccgc tgcgcgtctg aatcaagaaa acccgtattt tcatgtatca
     3721 aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg taactatgaa
     3781 accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta ttgaagttct
     3841 gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc gcataaccat
     3901 cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact tgcccggaac
     3961 agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga aagtgttatt
     4021 tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg gtgctaacgg
     4081 ttttgtcagt aaatgcaatg atcagaatga tatttttcat gccgttcaga tgatcctctc
     4141 cggatacacg ttttttccca gcgaaacgct taactatata aaaagcaata aatgtagtac
     4201 gaatagttca acggtcactg tgctatctaa tcgtgaagtg accatattac gttatctggt
     4261 tagcggatta tctaataaag aaattgccga taagttatta cttagcaata aaacagttag
     4321 tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag agctt
//
LOCUS       DAZGCS010000225         4337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGCS010000225 DAZGCS010000000
VERSION     DAZGCS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.228"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(147..491)
                     /locus_tag="ACHK01_004963"
     CDS             complement(147..491)
                     /locus_tag="ACHK01_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099742.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFNKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(520..687)
                     /locus_tag="ACHK01_004964"
     CDS             complement(520..687)
                     /locus_tag="ACHK01_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099743.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(723..1022)
                     /locus_tag="ACHK01_004965"
     CDS             complement(723..1022)
                     /locus_tag="ACHK01_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099744.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(1180..1458)
                     /locus_tag="ACHK01_004966"
     CDS             complement(1180..1458)
                     /locus_tag="ACHK01_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO8099745.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(1458..2141)
                     /locus_tag="ACHK01_004967"
     CDS             complement(1458..2141)
                     /locus_tag="ACHK01_004967"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO8099746.1"
                     /translation="MASLERELIRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEP
                     DDLSEWADMHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEP
                     GNSPVIPDGLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSL
                     PVTPDGWISCSELMPDKLIPVMVMYEDGEMWSAMWNGNRWDDGTEYPDPHSVTHWREM
                     PAAPQQEVK"
     gene            complement(2115..2240)
                     /locus_tag="ACHK01_004968"
                     /pseudo
     CDS             complement(2115..2240)
                     /locus_tag="ACHK01_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(2251..3006)
                     /locus_tag="ACHK01_004969"
     CDS             complement(2251..3006)
                     /locus_tag="ACHK01_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099747.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHVNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            complement(3008..3415)
                     /locus_tag="ACHK01_004970"
     CDS             complement(3008..3415)
                     /locus_tag="ACHK01_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8099748.1"
                     /translation="MSEINSQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            complement(3412..3633)
                     /locus_tag="ACHK01_004971"
     CDS             complement(3412..3633)
                     /locus_tag="ACHK01_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8099749.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(3732..3947)
                     /locus_tag="ACHK01_004972"
     CDS             complement(3732..3947)
                     /locus_tag="ACHK01_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8099750.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(4024..4215)
                     /locus_tag="ACHK01_004973"
     CDS             complement(4024..4215)
                     /locus_tag="ACHK01_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO8099751.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            complement(4188..4334)
                     /locus_tag="ACHK01_004974"
     CDS             complement(4188..4334)
                     /locus_tag="ACHK01_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO8099752.1"
                     /translation="MTFVYSVTKRGWVFPGLSVIRNPLKAQRLAEEINNKRGAVCTKH
                     LLLN"
BASE COUNT         1050 a         1095 c          936 g         1256 t
ORIGIN      
        1 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
       61 agccgcattg atgcggcgat ggtaggtctg gatatcattg agcaatgaac aggcctcacc
      121 gagtgtgagg cggtgttatt tcatggttag tccttgcgta gctcgctgat tcttctgtaa
      181 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
      241 tcccattcgt tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gttgaatgaa
      301 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
      361 tggggagcgc cgaagtgatg accatagata ttctttttca gtccacatgc gattgaggca
      421 agagttgcgc tactgatgcc aacatcgcca gtcgattgcc atttcaaaac cttcatagcc
      481 aaatctgaca tttcttgtct ccaataaaaa aaaccgccat caggcggctt ggtgttcttt
      541 cagttcttca attcgaatat tggttacgtc tgcatgtgct atctgcgccc atatcatcca
      601 gtggtcatag cagtcgttga tgtcctctgc ttcgataact ctgttgaatg gttctccatt
      661 caattcacct gtgactcgga agtgcatttg tgatttcccc aaaagatgct tgagtgcgct
      721 tcttattcga ttcgcacacc tggtatttcg ccttttgaaa tggctaagtc ataaatttgc
      781 gcagcactat acccatctcg catccatgaa tctaaggcgc gaatagcctc gctacgcttt
      841 ttatcttctc tttcattttt gatatcaacg aggacatcaa cgcaattaag gcatatgtgg
      901 attttgtcct tacattcgat catggcagct ttaccatgat ttccgccaca cagtgagcat
      961 aaatcttcag ggtctggctg gtatttctgt aacgttaaag ggttgaatgt tgaacaggcc
     1021 ataatcatct ccataaaaca aaactcgccg tagcgagttc agataaaaga aatccccgtc
     1081 agtgcgagga ttgttattgt cttttcttcg tgcatttgtc gcactttcgg caccatccag
     1141 ataggcacat ccgtccgcaa ttaacacata taggccacat catttttcct cttttggttt
     1201 atgaatctga atggtcatgc cgctttgagt ggtgactaca atgacataac caggctgaag
     1261 actgttaaga ttgaatgctt cgtaaaacga atccaaggcc agcgcttttt tattctttcg
     1321 gttccaccaa cgccatccct tgctacaggc tacactgaca atccactgcc cactcctgta
     1381 agccatataa aaccagatga gcaaaacctg aaggaatgct atccagtcaa taatcgtata
     1441 tttcgcgaag gagtccatca cttcacctcc tgctgcggtg ctgctggcat ttcacgccag
     1501 tgcgtaactg agtgcggatc cggatattcg gtgccatcat cccagcgatt gccattccac
     1561 attgcagacc acatctcacc gtcttcatac atgaccatta ccggaattaa cttatccggc
     1621 attagctcac tacagcttat ccaaccatcc ggagttaccg gaagcgagaa cggcagcaca
     1681 tctctgtgaa caagtttttg ctgtgacagg ttatccagaa ctttctgtac tgctgcatca
     1741 ccgaatacac caagcgcatc tgccataact cctacaacct gataagcctc agcgcatacc
     1801 gtggataaac catccggaat taccggagag ttgccgggtt ctttaatgtg caagcgaggc
     1861 tcaccatctt ttggttcagg ccactggcgc tccatgttga tcttcaattt atcttccata
     1921 gcagcggtaa tttcagcatc gctgatgcca gcacggcgct gtgcatccca caacaggaaa
     1981 tgcatatcag cccactcgct gagatcgtct ggttcggctg cggcttccag agcctctttt
     2041 gagaggtgtt tcagtggacc aatggggcca acgcagccaa atgtggagtc agaccatttg
     2101 gcatgctcgt ggcgaatcag ttcgcgttcc agtgatgcca gtgcaattcg tgccagttcc
     2161 atttgttcgc cacgagtaag cccgttttca agcggatttt taatgaacaa ttcaatacgt
     2221 tctttggtaa tagtggtcat ttgttagtcc ttaaagtgct agttgcaatt gcatttcaaa
     2281 gcggtcgcgt tgttcacaat acgcaagaga accagggcta ttgtgtgcct caatccgttc
     2341 taccattaat gctgcgcgtg tctctttact tgcaggtgca taagccccag accaggcttt
     2401 atcaataccg atgtttcgag cgacgttcgt actatctgcg ctggctaagg gtaattttgt
     2461 gaatatcagc ggatttaaca tgcgcaatcc atgtagtttc gtaaccggct gaccatgccc
     2521 atcaacaatg tgacgaatca ggtctttcat tctggctacc gcaagagttg ggcgctttac
     2581 gtcatagtcg ccacaactac cgatagccac tcgcggaaac tcattgcaca aatgaataaa
     2641 tcgctcgtca ctttcattca cgtgccacac tggaacgcca gctagttttc cgtgaggcca
     2701 ctcattcaga agcgcatcat tttcctcctc tccgccatca ataacatccg ggataatggc
     2761 aaaatcgaat cctgggtgat tcttccagcg agcaacaaac tcgtagtaat cgctccagtc
     2821 gattttgttt ttgccagctg ctttccaggc ggtgaatgca ccgttgtcca gcgcgaacga
     2881 ctgacagtat tcagccgcga gattgatctg gcctgaatgc gcaaaactga taaacgcatg
     2941 tcgccctttc catgctctca ttgcgcacgt atcaggagta ataggcccac cgtggtagtg
     3001 aatcatctca ctctcctttg atgcgaatgc cagtagcgcg gattgcatcg atgacttcag
     3061 aaactttgta tgccattacc gtttggtaat catcgtgaaa atctgttcga tgaagcatgc
     3121 tgctacgttc cgggagcagt atttcccgcg cttccagttc tgcaatgcgc ttttttgctg
     3181 cttccagttc atccagtaat tccagcacgg tagccggatt agccttggca acaaaatccc
     3241 ggactggctt acaatcaatc tccgcaatgg gttgatacga tgtgtagcca tgctgtcttg
     3301 tataactacc gtgacgaata acgaaaaaat caccatttat ttttttagcc tgccacttat
     3361 cttcaccggc tttctctgcc gcttcacgca gtgcctgaga gttaatttcg ctcacttcga
     3421 acctctctgt ttactgataa gctccagatc ctcctggcaa cttgcacaag tccgacaacc
     3481 ctgaacgacc aggcgtcttc gttcatctat gggatcgcca cactcacaac aatgagtggc
     3541 ggatatagtc tggtagttca gacgacgcat ttttattgct gtattgcgct gtaattcttc
     3601 aatttctgat gctgaatcaa tgatgtccgc catctttcat taatccctga attgttggtt
     3661 aatacgcttg agggtaaatg cgaataataa aaaaggagcc tgtagctccc tgatgatttt
     3721 gcttttcatg ttcaccgttc cttaaaaacg ccgtttaaca tgccgattgc caggcttaaa
     3781 tgagtcggtg tgaatcccat cagcgttacc gtttcgcggt gcttcttcag tacgctacgg
     3841 caaatgtcat cgacgttttt atccggaaac tgctgtctgg cttttttgat ttcagaatta
     3901 gcctgacggg caatgctgcg aagggcgttt tcttgctgag gtgtcattga acaagtccca
     3961 tgtcggcaag cataagcaca cagaatatga agcccgctgc cagaaaaatg cattctgttg
     4021 ttgtcatgcc gggtctctct cgtttgcttc tgctttcgcc gccatcattt ccagcttttg
     4081 tgaaagggat gcggctaacg tatgaaattc ttcgtctgtt tctactggta ttggcacaaa
     4141 cctgactcca atttgagcaa ggctatgtgc catctcgata ctcgttctta attcaacagg
     4201 agatgctttg tgcatacagc ccctcgttta ttatttatct cttcagccag ccgctgtgct
     4261 ttcagtggat ttcggataac agaaagaccg ggaaataccc agcctcgctt tgtaacggag
     4321 tagacgaaag tgatcgc
//
LOCUS       DAZGCS010000226         4249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGCS010000226 DAZGCS010000000
VERSION     DAZGCS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.229"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..212)
                     /gene="ariR"
                     /locus_tag="ACHK01_004975"
                     /gene_synonym="ymgB"
     CDS             complement(<1..212)
                     /gene="ariR"
                     /locus_tag="ACHK01_004975"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO8099753.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKA"
     gene            complement(241..513)
                     /locus_tag="ACHK01_004976"
     CDS             complement(241..513)
                     /locus_tag="ACHK01_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO8099754.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(556..792)
                     /gene="ycgZ"
                     /locus_tag="ACHK01_004977"
     CDS             complement(556..792)
                     /gene="ycgZ"
                     /locus_tag="ACHK01_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO8099755.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1106..2317
                     /gene="bluF"
                     /locus_tag="ACHK01_004978"
     CDS             1106..2317
                     /gene="bluF"
                     /locus_tag="ACHK01_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO8099756.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2522..3253
                     /gene="bluR"
                     /locus_tag="ACHK01_004979"
     CDS             2522..3253
                     /gene="bluR"
                     /locus_tag="ACHK01_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO8099757.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3474..3878
                     /locus_tag="ACHK01_004980"
     CDS             3474..3878
                     /locus_tag="ACHK01_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO8099758.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3931..4041
                     /locus_tag="ACHK01_004981"
     CDS             3931..4041
                     /locus_tag="ACHK01_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099759.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
BASE COUNT         1261 a          876 c          837 g         1275 t
ORIGIN      
        1 tcagctttga gaatatcact tgttgtttcc agggagtgta tcagacttaa gataatattt
       61 ttattattaa cattatcgcc tgaaagcatt aaattggtga cagcctgccc taacactgct
      121 gactcttctt ctaacaaatt acccgaactg cgaaagtaac ttgctaacgc tttatcagtt
      181 attgcattat gaattgtagt atcttcaagc atggtaacct ctcatcttac ttatgaaatt
      241 ttaatgtatt ctgtttattt tcttaccatt gaacatactt ttattgtcag ctgtcgctgt
      301 tcttcggttt ggtcctcctg aatcactatt gctaaaatca cgaattatgc tgtcaattaa
      361 atcgcgtcgg aaagcatctt cctcagccga tttcattacg agtaattgct taataagagt
      421 ctgcaatgaa aaatttatct tcatgcgcag tatctggatc accgcctggc cagtaatttc
      481 atattttata cgttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat
      541 aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc
      601 ctcttcccca gtcgcgtgtt ccagtcggca aagaagtttg gcacaaagcg actttcgact
      661 cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt
      721 aggcaagtta gcttttgcaa agtaacgagt gattgctccc gcagaatcta aagtcactga
      781 attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg aggtaacaat
      841 agcaaacttg tacgaaatat gtgtacagaa tgataaatta attttttgca attagtgcta
      901 atgcatataa aacaatgtgt tatgataaaa catattgcat catttacctg acaatctata
      961 caagattttt cgccaactag tgttcaactt tttccatttt ttatcaatgt aaagaaacgg
     1021 ttatgttttg gtattaaaag aaagacacaa gaagatatgt ctgttaccgt cttactctcg
     1081 cctcacccat taccctggat tgttaatgct taccaccctt atttatcgta gccatatacg
     1141 tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg atagcaaatc gcaggaacat
     1201 gcagtctgac gtaacaggga tcttactgtt taatggttct cattttttcc agcttctgga
     1261 aggtccggaa gaacaggtta aaatgatata tcgggctata tgccaggatc cacggcacta
     1321 taatattgtt gagctgctgt gcgattacgc gcctgctcgc cgttttggca aagcgggaat
     1381 ggaattattt gatttgcgcc tgcacgagcg agatgacgtt ttacaggccg tattcgacaa
     1441 aggcacatca aaatttcagc taacttatga tgacagagcg ctacaatttt ttcgtacttt
     1501 tgtccttgca accgaacaat caacctattt cgagatccct gccgaagact cctggctttt
     1561 tatcgctgac ggatctgata aagaacttga ttcctgtgcc ctttcaccaa ctataaacga
     1621 ccactttgcc tttcatccta ttgtcgatcc cttatcgcgg cggataatcg cttttgaagc
     1681 cattgtgcaa aaaaatgaag atagcccatc agccatagcg gttgggcagc gtaaagacgg
     1741 ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca ttcacgatgg cacacgcact
     1801 tgagctcggt gataaaatga tttcaatcaa tctattacct atgaccctgg ttaacgaacc
     1861 tgacgcagtc tcttttttac ttaatgaaat aaaggccaat gctctggtgc ctgaacaaat
     1921 catcgttgaa tttactgaaa gtgaagtcat atctcggttt gatgagtttg ccgaagcgat
     1981 taaatcgcta aaggctgctg gtatcagtgt agcaattgat cattttggcg caggttttgc
     2041 tggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat
     2101 taccaatgtt cataaaagtg ggccacggca ggcaattatt caggcgatca taaaatgctg
     2161 tacatcactt gaaattcaag tcagtgctat gggcgtggca acaccagaag agtggatgtg
     2221 gcttgaatcc gcaggaattg agatgtttca gggagatctg tttgctaaag ctaaattgaa
     2281 tggtatccct tccatagcgt ggccagagaa aaaataattt tcagcacatt ctttcacatg
     2341 atttcagtaa atcatcgtga aaatgaatac gttacgttta aatttaatcc acattaaaaa
     2401 taatgtttca tccaatttag ctgaaatctt atttatactt tgtacaattt tagcgtatat
     2461 tgtacaaagt attattgggt cgtgtacagg cgacggagat ttgtgaccgc aaggaggaat
     2521 tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc ctgtcactct
     2581 ccgggcctgg caacgccgct acggtttgtt aaaaccacag cgcagtgaag gcggacaccg
     2641 actctttgat gaagaagaca tacaacgcat cgaagagatc aagcgttgga taagtaatgg
     2701 cgtccctgta ggcaaagtta aagcattact ggaaaccacc agccaggata cggaagatga
     2761 ctggagccgc ctgcaagaag agatgatgtc aattcttcgc atggctaatc ctgccaaact
     2821 acgcgcgaga attatttcac tgggtcgaga gtacccagta gatcaattga ttaatcatgt
     2881 ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca ttatgagcag
     2941 tatgtttgac ggcgcattaa ttgaatacgc agcaacctcg cttttcgaaa tgcgccgtaa
     3001 gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa gagagagcac gattgtggct
     3061 ggaagcatgg cgtttatcat tgtcaggatg gcacatttct gttcttgctg atcccattga
     3121 atcgccgcgc ccggaactgt tcccgacgca aacattgatt gtctggacag gcatggcacc
     3181 aacgagaagg caaaacgaac ttttacaaca ctggggtgag caaggttata aagtcatctt
     3241 tcatgccccc taatttcacc tttatcccgc cccttagaca gaatgttata atctgaaccc
     3301 acaacagcgt ttgtaccgga gctggtccgt tgcaaaatgc tcaccgggag aattcagata
     3361 ttccctctat cacactctgt acatatcaca gccaaaggac agaccagatt gaagtggtaa
     3421 aaccgatcgt gcacactacg cagtctcaca actaacaaga ggttattatc ctgatggatc
     3481 aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac ttctcctggt
     3541 tttattcaga attaaaacat caccgtattg cacattacat ttattatctg gctacggata
     3601 acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc aggaacctgt
     3661 taaaagttag tacgaccaag aaccctgcta aaataaaaga ggccgcattg cttcatattt
     3721 gcggaaaatc tacatttcgg gaatactgtt caacactagc aggcgcgggc gttttccggt
     3781 gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat acgcttttat
     3841 atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggat gcttaagaaa
     3901 tgcttcataa ttcagtaagg cattagcata atgggaataa aagtacagag actatcccta
     3961 tggatgataa atactgtcct tttattgcta cccataaata atcaccatgc caatactatc
     4021 aaattgatat ttgaaatgtg atcacttgac tttctatacg ttattttata acggttaata
     4081 tatttataaa aacaacgggt gtgccatacg cccgtttcaa tacttaacgc acatgtgttt
     4141 tggtttagtc atcatccagt tatatgtatt ttagccagga acaggttaaa cctttcctat
     4201 ataactcaaa aattgaaacc ttattctcat gtcatgctta tattcatta
//
LOCUS       DAZGCS010000227         4186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGCS010000227 DAZGCS010000000
VERSION     DAZGCS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.230"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..62)
                     /locus_tag="ACHK01_004982"
     CDS             complement(<1..62)
                     /locus_tag="ACHK01_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HFO8099760.1"
                     /translation="MSIDALRWAKKVKTGSSSSKS"
     gene            complement(34..585)
                     /locus_tag="ACHK01_004983"
     CDS             complement(34..585)
                     /locus_tag="ACHK01_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099761.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRITFNDGEVDQRALGVNRVKIFERWKAIDTRDKRKKFTALIPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPSVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQISVWGSYEYRCTTMG"
     gene            complement(569..796)
                     /locus_tag="ACHK01_004984"
     CDS             complement(569..796)
                     /locus_tag="ACHK01_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8099762.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            874..1281
                     /locus_tag="ACHK01_004985"
     CDS             874..1281
                     /locus_tag="ACHK01_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099763.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            1471..1626
                     /locus_tag="ACHK01_004986"
     CDS             1471..1626
                     /locus_tag="ACHK01_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8099764.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            1628..1756
                     /gene="ydfB"
                     /locus_tag="ACHK01_004987"
     CDS             1628..1756
                     /gene="ydfB"
                     /locus_tag="ACHK01_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO8099765.1"
                     /translation="MDFDTIMEKAYEEYFEGLADGEEALSFNEFKQALSSSAKSNG"
     gene            1786..2004
                     /gene="ydfC"
                     /locus_tag="ACHK01_004988"
     CDS             1786..2004
                     /gene="ydfC"
                     /locus_tag="ACHK01_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO8099766.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     KLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(2008..2172)
                     /locus_tag="ACHK01_004989"
     CDS             complement(2008..2172)
                     /locus_tag="ACHK01_004989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099767.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     YLITNLSFVG"
     gene            2570..2758
                     /gene="dicB"
                     /locus_tag="ACHK01_004990"
     CDS             2570..2758
                     /gene="dicB"
                     /locus_tag="ACHK01_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8099768.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            2755..2943
                     /locus_tag="ACHK01_004991"
     CDS             2755..2943
                     /locus_tag="ACHK01_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8099769.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            3036..>4186
                     /locus_tag="ACHK01_004992"
     CDS             3036..>4186
                     /locus_tag="ACHK01_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO8099770.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAP"
BASE COUNT         1162 a         1020 c          957 g         1047 t
ORIGIN      
        1 gacttactgg atgaactgcc ggttttcacc tttttagccc atcgtagtgc atcgatactc
       61 atacgaaccc cagacagata tttgtttatc tgcaaagtaa tattgatact gctgacgata
      121 cgcatgcttg aaagcaatag ctttttctat aagctcgtca gtctcacgtt ccacaacaga
      181 tggatccgca aaaagcagcc cggactccac cacatcgcca tattctttgt ttaatccggc
      241 gatcatgtac gtaatgcttt ttccatcact gatctcacga tacaacctga aatcactaat
      301 tcggatagcc tccataattg ccggaatcag cgccgtgaat tttttccgct tatccctggt
      361 gtcgatagct ttccagcgtt cgaatatctt cacccggtta acgcccagtg cccgttgatc
      421 aacctcgcca tcattaaacg tgatgcgttg aacatcgatg ttcgggcgtt ctttcagagc
      481 ccagaatgct tccgtgatta atatcgtcgc ctgctcctgt gtcattcctg gtcgacatac
      541 ccaggcatcc agagcctcac aaacctgttc aggggtgatt ttcattgttc aaccgccccg
      601 cccgctttgc cttacgatat tcgtcataaa ctttggggtc gtactgaagt tccccgccgg
      661 atgcctcttg caggcgcatc gcgcgacctt caggaaccag ttccccccat tgagaaacag
      721 cagatggatc aacaccagca gctttcgcta ctttggcttt cgtcccataa aaattaatta
      781 cgtctgattt aaacatcacc cctccaaagt tgagttttct caatagtaat cactcaagga
      841 atctcaagtc aagggtgatt aagatatcta aatatgaacg agaaaacttt aggtcaacga
      901 attagagaaa gacgcaaaca ggttggttta agtcaaaacg atttaagcaa agccgctggc
      961 gtatctggct catcaatttc actatgggaa agcgaccata cagccccgcg cgggcaaaat
     1021 ttgcatcgcc tggctgaggt attgcaatgt tcaccaacct ggatactgtt tggtgacgag
     1081 gataaaacac cagatccacc agttgcactc aacagcgcct tagacttatc ggaagatgag
     1141 ttggagatgt tgcgattgta tcgcgcactt ccaaaatcag agcagcaagc acaaatcagc
     1201 gaactccgtg cccgcgttga gaattttaat cgcctattca ccgagctact agaagctcgc
     1261 aaacgtaaca aacatcagta acccccttca caaattttaa agccttacat ttcaatgtat
     1321 tggctttatt ttgcattaaa tattgagctt tctcattaaa aatacttgac caacattcat
     1381 gagaaaacta aattaccacc catcaagaca ccgcacggtg ttctcagcaa acagttccgc
     1441 tacccggcgt taaggggaaa tgaggtcagc atggatacta tcgatcttgg caacagcgaa
     1501 tctctggtat gtggcgtgtt ccccaaccag gacggcacgt tcaccgcgat gacgtatacc
     1561 aaaagcaaaa cgtttaaaac cgaaaatggt gcccgtcgct ggctggaaag aaactcaggt
     1621 gagtgatatg gatttcgaca caatcatgga aaaggcttat gaagaatact tcgaaggtct
     1681 tgccgacggc gaagaagctc tcagcttcaa cgaatttaaa caggcgcttt ccagctcggc
     1741 aaaatctaac ggctgataag cgaaacagca ccgcgaggaa tcagtatgca gaaacgagaa
     1801 cccgtcatca tcgcaccaga ctataccgat gatgaacttt atgagtggat gcgccagaaa
     1861 attaatgcag cgcaggatct gaaatgggcc aatgaagcca gggctaagca ggctgaaaaa
     1921 ctgtccgctc tggagcagga tatcaccaat ctggaaaaag cagcggcatt aagcattgcc
     1981 agaatgatta catacccgcg ttaatagcta accaacgaag ctaaggttgg taattaagta
     2041 gttctccacg ggtgaggtgg agtgcgtgcg ccggacgcgg gtgagcatcc ggcactgaca
     2101 gtttactgaa aggatatttc cctgaaaagt cagaccataa cgcgaaagcg cacggcgagg
     2161 tagctggttc atagatagcc tgtcgttaaa ttttcgtcga ccgtgcgctt ccggttgtgg
     2221 caacccgcga aatggtgcgg cggtaagtat ggcggggtta tttcttcccc gttgaggaca
     2281 ccgggttgtc aggttgacca tacgcttaag tgacaacccc gctgcaacgc cttctgttat
     2341 caattttctg gtgacgtttg gcggtatcag ttttactccg tgactgctct gccgcccttt
     2401 ttaaagtgaa ttttgtgatg tggtgaatgc ggctgagcgc acgcggaaca gttaaagcta
     2461 aaaacagcgt tatgggtgat tctgtattcc ggcgttaatt gttaactggt taacgtcacc
     2521 tggaggcacc aggcaccaca tcacaaaatt cattgttgag gacgcgataa tggaaacgtc
     2581 actaccaaac gttaatacgt ctgaagggtg ttttaatatt ggtattctgc tcagtaaccg
     2641 ggagtttact gaagacgcca tcaggatgag aaaatatgag ccttatcttc tcaatgataa
     2701 ttccatactc tccagaattg cccttcttga acttggcatt ttcggagggc agcagtgagt
     2761 tcagcgtttg cactgatgat gacggttttt cttataacag gtgagccaca gaatgtgatt
     2821 accggaattt atgccagtaa agaatcctgc catcaggcaa gagacgagca aaaaatttcc
     2881 ggtgaatgtc tcccgttaaa caaagtatcg ctgtacctga ataacgaaat accggctgga
     2941 taacccgcca gccgtattaa cgccataccc gttgattaag catgccagca atggcaggga
     3001 ttcgtacaac cttaaaatag ttatgaggtt tatcaatgag cactgataaa gaagaaattg
     3061 cactgtatta cgaagccaaa aatgacaaag tcagaaaacg ccttgggatt aaaggcggtt
     3121 tttactggcg cacagcaaaa aaattatcgg ttgcaatatc acggggtgtt gtcgcaatgg
     3181 acgatgctgg atttgacgaa gaggatttca aaaaacctgt tcgcgtgaat ttgcccattg
     3241 ttaatgacct gccgcctgaa ggtgtgttcg atactgaatt ctgcaaccgc tatgaaaaag
     3301 gcggggaaga tggcatcaca atgatattta tagcgccttc cccctcagtt caggacaaac
     3361 cagccagctc tgacaatacc aacgtcaatg gcgaagacat ggctgagatt gaggataata
     3421 tgctcctgcc gatttccggt caggaactgc ccattcgctg gcttgcgcaa catggcagcg
     3481 aaaaaccggt aacgcacgtt tcacgggaag aacttcaggc attacatatc gcacgagctg
     3541 aagaactgcc tgctgttact gccctggcta tttcccacaa cacaaagctg ctcgacccgc
     3601 tggagattcg cgaccttcac aaactggtac gcgacacaga caaagttttc cctaatcccg
     3661 ttaattccag tctggggtta atgactgctt ttttcgaagc atacctggac gctgactata
     3721 ccgatcgagg tctgctgaca aaagagtgga tgaaaggaaa tcgtgtttta cgcatcagcc
     3781 gcacgccatc cggcgctaat gctggcggag gaattcttac cgatcgcggt gaaggttttg
     3841 tccacgatga tgcgtcagta gaacgtgacg ttgccgctgg cgttctggcc cgttcaatgg
     3901 acatcgatat ttacaatcca catccggcac acgccaaacg cattgaagaa atcgtttcag
     3961 agaataagcc gcccttttct gtttttcgtg acaaattcat cgccatgcct ggtcacctgg
     4021 attattcccg cgcgatagtg gttgcgtccg tgaaagaagc accaattggt atcgaggcta
     4081 ctccccaccg tgttaccgaa tatctgaaca aagtactgac cgaaaccgac catgccaacc
     4141 ctgatccaga aatcgtggat attgcctgcg gtcgctcctc tgctcc
//
LOCUS       DAZGCS010000228         4181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGCS010000228 DAZGCS010000000
VERSION     DAZGCS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.231"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..397)
                     /locus_tag="ACHK01_004993"
     CDS             complement(<1..397)
                     /locus_tag="ACHK01_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO8099771.1"
                     /translation="MATLMEKDALLNGASQCITFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRET"
     gene            complement(542..1891)
                     /gene="lysC"
                     /locus_tag="ACHK01_004994"
     CDS             complement(542..1891)
                     /gene="lysC"
                     /locus_tag="ACHK01_004994"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO8099772.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(1983..2179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2416..4065
                     /gene="pgi"
                     /locus_tag="ACHK01_004995"
     CDS             2416..4065
                     /gene="pgi"
                     /locus_tag="ACHK01_004995"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO8099773.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1072 a         1062 c         1042 g         1005 t
ORIGIN      
        1 ttgtctctct tttggctaat tcaatcagag atgatttacc tgctccgggt tgcccaccta
       61 aaacaaaacc aatgggatgc tgttgcggtg tgacttcatg atttgtcaga cgctgccaaa
      121 tagaatcaaa agatgcttta ttttctgaat gcgtagtatt gttgaattca tgctgatatt
      181 gagttcttac ctgctgaagg agaatcttac cttgtgtaaa atcaacaagt tcagcggggg
      241 tagaataaag ataatcgcga tagctgacta atcgcttgag agcatcgcct gaatcctgat
      301 gcgacgaaac tgagcaatta tcaatgatat ttgataaaaa cgtgatgcac tgactggctc
      361 cgtttaaaag tgcatctttt tccattaaag tagccattaa cactcctgtt aaatgatatt
      421 tttcggtgat tataatactg ttctacagga gtgattttgc ttttcagata ttatttaatc
      481 tatgttttca gtgaggatgg cgtataacaa tcggtgagcg ttatacgcca gtagggaaat
      541 attactcaaa caaattaaaa tgcagcttct gcaccacctg ctcggcatct tcgccgggca
      601 ccaggaagca caggttatgg ctggatgcgc cgtaacaaat catgcgaatg ttgaacggtt
      661 ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt tgacaggtca ttgccaatca
      721 acgcgaccag cgccagacct tcttctactt ccacccggca cagtgcggaa agctccatca
      781 gcagagattg cgtcagcaac gtatcgccag tggaggttga accggtggta tcaagggtta
      841 atgccacgct cacttctgac gtggtgatta agtctaccga aatattatgc cgcgcgagga
      901 tgccgaaaac ttccgcgagg aagccgcgag aatgcagcat attcaggctg tgcaaagtga
      961 gcagagtctg attgcgacga agcgccagcg cgcggaacag cggcggattt tcagttttat
     1021 tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga gccgacaaag actgggatat
     1081 cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac ttttgcacca aaagttgcca
     1141 tctctgccgc ttcggcaaac gcgatttcat caatgcgttt tgctgcggaa actacgcgtg
     1201 gatcggtggt gtagatgccc gggacgtcgg tccagatatc aacacgagat gcgtgtaaag
     1261 cctccgccag caaggctgcc gtataatcgc tgcctccacg gccaagcgtc gttgtacgac
     1321 ctttattttc gctaccgata aatccctggg tgatcactaa gccgtcattg aggcgtggga
     1381 gcagctgcag cgcggccagt tccgccagcg cggctacatc tggctctgca cgaccaaatc
     1441 ggtcgttggt acgcatcact ttacgtacat caaaccactg tgcctgaaca tcgcgttcgc
     1501 gcaggatttc gacaaacagc agggtcgaca tcagctcgcc gtggctgacc agctcatctg
     1561 tcagcgccgg agacgttgcc agcgccgccg cttctgccag aacagtaatg ttctccagca
     1621 gacgttcaat ctcttcacgg ataacgttcg ggtaacgcaa acgttccaga atggcaaact
     1681 ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc aggttccagc ccttccgcta
     1741 aagcgaccag cagattagtg ataccagcag aagccgagag gactactaaa cgcacgttgg
     1801 catcagaaag cacaatatca gcgctgcggt tcatggcgtc aaaatcagct acgctggtac
     1861 cgccaaattt ggagacaaca atttcagaca taactacctc gtgtcagggg atccattttc
     1921 agccttggca caagggaaga gcggaagacg ggtgggcgca gagcgatact tcgctactat
     1981 tttcacccag aagtgctcca ccacttgcga aacgcccgac tgcgaacgct tctggtgaca
     2041 acccagggga ttcagcccct gtagccgatg atgaacgtgg ccagccgttc aatcacctcg
     2101 gcgatgcacc ccctcaggtg ttatcacagg actggctcct ccaacaccgt tacttgggca
     2161 acgcgcctct tctggcctgc gctagcgcag gtagtacatt tataaataaa gggtgagcgg
     2221 ggcggttgtc aacgacgggg tcatgcggat ttttcatcca ctcctggcgg tcagtagttc
     2281 agctaataaa tgcttcactg cgctaagggt ttacactcaa cattacgcta acggcactaa
     2341 aaccatcaca tttttctgtg actggcgcta caatcttcca aagtcacaat tctcaaaatc
     2401 agaagagtat tgctaatgaa aaacatcaat ccaacgcaga ccgctgcctg gcaggcacta
     2461 cagaaacact tcgatgaaat gaaagacgtt acgatcgccg atctttttgc taaagacggc
     2521 gatcgttttt ctaagttctc cgcaaccttc ggcgatcaga tgctggtgga ttactccaaa
     2581 aaccgcatca ctgaagagac gctggcgaaa ttacaagatc tggcgaaaga gtgcgatctg
     2641 gcgggcgcga ttaagtcgat gttctctggc gagaagatca accgcactga aaaccgcgct
     2701 gtgctgcacg tagcgctgcg taaccgtagc aataccccga ttttggttga tggcaaagac
     2761 gtaatgccgg aagtcaacgc ggtgctggag aagatgaaaa ccttctcgga agcgattatc
     2821 tccggtgagt ggaagggtta taccggcaaa gcaatcactg acgtagtgaa catcgggatc
     2881 ggcggttctg acctcggccc gtacatggtg accgaagcac tgcgtccgta caaaaatcac
     2941 ctgaacatgc actttgtttc taacgtcgat gggactcaca tcgcggaagt gctgaaaaaa
     3001 gtaaacccgg aaaccacgct gttcctggta gcatctaaaa ccttcaccac tcaggaaacc
     3061 atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag cggcaggtga tgaaaaacac
     3121 gttgcgaaac actttgcggc gctctcaacc aatgccaaag ccgttggcga gtttggtatt
     3181 gatactgcca acatgttcga gttctgggac tgggtcggcg gccgttactc tttgtggtca
     3241 gcgattggcc tgtcgattgt tctctccatc ggctttgata acttcgttga actgctttct
     3301 ggcgcacacg cgatggacaa gcatttctcc accacgcctg ccgagaaaaa cctgcctgta
     3361 ctgctggcgc tgattggcat ctggtacaac aatttctttg gtgcggaaac tgaggcgatt
     3421 ctgccgtatg accagtatat gcaccgtttc gcggcgtact tccagcaggg caatatggag
     3481 tccaacggta agtatgttga ccgtaacggt aacgttgtgg attaccagac tggcccgatt
     3541 atctggggtg aaccaggcac taacggtcag cacgcgttct accagctgat ccaccaggga
     3601 accaaaatgg taccgtgcga tttcatcgct ccggctatca cccataaccc gctctctgat
     3661 catcaccaga aactgctgtc taacttcttc gcccagaccg aagcgctggc gtttggtaaa
     3721 tcccgcgaag tggttgagca ggaatatcgt gatcagggta aagatccggc aacgcttgac
     3781 tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga ccaactccat cctgctgcgt
     3841 gaaatcactc cgttcagcct gggtgcgttg attgcgctgt atgagcacaa aatctttact
     3901 cagggcgtga tcctgaacat cttcaccttc gaccagtggg gcgtggagct gggtaaacaa
     3961 ctggcaaacc gtattctgcc agagctgaaa gatgataaag aaatcagcag ccacgatagc
     4021 tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg gttaatttaa aaaccttaat
     4081 aacaatgccg acgttatgtc ggcattgttt tatcagataa atccccttgt ctgtgattta
     4141 acggaaatca taccgtgagg ttaatcctaa aatagatttt t
//
LOCUS       DAZGCS010000229         4162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGCS010000229 DAZGCS010000000
VERSION     DAZGCS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.232"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..405)
                     /locus_tag="ACHK01_004996"
     CDS             complement(85..405)
                     /locus_tag="ACHK01_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO8099774.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            complement(465..1805)
                     /gene="escN"
                     /locus_tag="ACHK01_004997"
     CDS             complement(465..1805)
                     /gene="escN"
                     /locus_tag="ACHK01_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO8099775.1"
                     /translation="MISEHDFVLERYPRIQRVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYRIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGDEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYADPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1789..3816)
                     /gene="escV"
                     /locus_tag="ACHK01_004998"
     CDS             complement(1789..3816)
                     /gene="escV"
                     /locus_tag="ACHK01_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO8099776.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKKTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3813..>4162)
                     /locus_tag="ACHK01_004999"
     CDS             complement(3813..>4162)
                     /locus_tag="ACHK01_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO8099777.1"
                     /translation="LLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSNVF
                     ITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTFQQ
                     HVQIIERVRRMTS"
BASE COUNT         1292 a          963 c          742 g         1165 t
ORIGIN      
        1 tcattaaaac tttcttctcc ctgcggccaa tcatctgacg gctgaaaaga aacaggctct
       61 atcaaatttc tttttagaga aatgctagtc aaagtaatgt tcctttatgg caaaagataa
      121 cttctctatt gatttattaa catgttgtag atcctctttt gttcgttttt gtgaatcgag
      181 caatgaacgc ttttctttag aaagtgagga ttttttctca tataatcgct gcttttgctc
      241 atcaaatgca ttgtttttaa tctggtattt atacaacgac actttatcca gattcaccag
      301 cgatgcacaa tacgcctgct tagaggcaat cgactcttta atggcttgct cgcagtcatc
      361 gagtttacca tccacctctt ttatctgaga gttaatctta gccattgatt ctctcaatcg
      421 attcgctctg cttttacgaa tagataaaat tctgtccaac atattcaggc aaccactttg
      481 aataggcttt caatcgtttt ttcgtaacta ctgatatctt tggtgctttg ttgtataaag
      541 ctctgaattg ctttcctgtt ttttattgcc ttatccgctt caggatcttg ccccatcgtg
      601 tactcaccaa tacgaataag cagctcaata tttttatagg ttgcgataag ctttttgcat
      661 tctgccgccg cacgcaaatg ctccgatgta acaacattat gcataacacg actggcgctt
      721 aaaccaatat caattgcagg aaaatgattt tcctctgcaa gttctcgggt aagcacaatg
      781 tgcccatcaa gaatagagcg gacttcatca ccaatgggat cattaacatt atcgctttct
      841 aacaacaccg tataaatcgc ggttattgag ccttttggcg caggccccgc acgctctaat
      901 aatttgggta acgaggaaaa aacactcgga gggaatcccc cccttacatc aggctctccc
      961 gatgcaagcc caacatcacg cgcggctcgg gcataacgag ttacggaatc catcattaat
     1021 aatacatttt tgccctgatc ccggaagaac tctgcaatgg ttgtcgccgt aaatgcggcc
     1081 ttcattcttt ccagcgcggg acggtctgac gttgtgacga ccagtacaca tttagaaagc
     1141 gtggattgag gtaaaagcgc gaggaattca tttacttcgc gaccacgctc gccgataagg
     1201 gcaaggacaa taatatccgc ggatgcgcca ttacaaatca ttcccaaaag cgtactttta
     1261 ccaacgccgg accccgcgaa aataccgata cgttgcccaa taccgcaagt aagtaaacca
     1321 tctattgccc tgacgccaag cgtaaatggc tgatcaataa cctgccttaa taaaggatcg
     1381 ggggggtcag catacaaact gcgttcaaac ggaagataag gtgcagtaat attgctcccc
     1441 attggtctgc ctataccatc aaccagtcgc ccaaggagtt cgtccccaac acgaatcttg
     1501 aactcatccc cctgataact tagccactgg ccacaataca tgccggaaat atgctcaaaa
     1561 ggcagcagga aaacctcatc ttcatcaata gcgatcacct cagctaaacg ttgactgggt
     1621 tctatcctgt aaaaggcgcc aatacgcgct tttggtaaac gcgccttgat aatcgtcccg
     1681 ccgatattgg taatcttgcc ttcatatctg gtagacgaat ttaatgataa tgtcggcaca
     1741 gtgctattga gcactctctg gatacgtggg tatctttcca atacaaaatc atgctctgaa
     1801 atcatttacc gttcctaata ctttaagttc gatattattc ccaacttcct gaaaagagag
     1861 cacggggact gacgggaacg aaccttcaat cattttcttc acgtagcgcc tgatatctaa
     1921 agcggtcaga atgacgccgt tccccgttgg attcacaata ttttttaaga agccaataat
     1981 ctgctcactt cgctccggtg agatattcaa atacgaacct gatgacgttt gtctgatcga
     2041 ctctcgtagc tcattttcaa tatcagagcc aataagccaa acattcagaa gtgtaccgcg
     2101 aacgctatag cgacctaaaa tatgccgacg tagggcgata cgaacgtatt cgcacaagat
     2161 aaccacatct ttttcttttg tggaccaaaa aataagcgtt tcaaaaatag ttcgtagatc
     2221 tctaattgag atattttcct ctacgagacg ctgaagaata tccacaatct tgctcagacc
     2281 aagctgacgc tgaagctctt tcacaagttc gttatatttc ctctccatga tgtccatcag
     2341 ataacgcgtt tcttgtacgc cgatgaactc tttggcatta gacaatacaa gtttctttag
     2401 caaaaacgtt atcttatcat ttgtttttgc ataaacatcc atacctgaga cgtgagctag
     2461 ttgttggtta gcctcattcg ttttatacac gatagagtta gccgaaagct tttcctggac
     2521 aaattcaaat gatatctctg catgcccagc ctcaaaataa acagtatcat ctgtcagagt
     2581 atctttgtat attgactctt gatagagcaa aaattctata gcgctatcat tgtttttaac
     2641 tggattaaca ataatcttag gcaatggaat tccactttcc tcaaagaaat tccaacgcat
     2701 aacctcaatc tgctgtgtaa tatctgagct ataaatatta ctacttaagt ttaaaatcaa
     2761 cggctcggca cccggtgaga tattttgact attagacaaa tcagctccca tagcgtcctt
     2821 attatttccg gttccaaacg tctttttctt ttgtaatttc caggcgatac ccaacaggca
     2881 aaccgccaga aacaagaaga ccaatgtagg aaagccagga ataatcgcca ttacgatcat
     2941 gagtgcggca ctgattaaaa aagggcgact attaacagaa acctgttgaa caatatcacc
     3001 tgcaaggttt tcttcttttt cgctttcacc gggaacacga gtaacaacaa cccccgcagt
     3061 gacggaaata ataagcgcag ggatctgggc aaccaaagca tcaccgacag acaatacaga
     3121 aaacaggcta agtgcttcac taaatggcat gtcaaattgc cacattccaa tgagcacgcc
     3181 accaaacagg ttaactaaaa caatgatgat accggcaata gcgtcccctt ttacaaactt
     3241 catcgccccg tccattgaac cataaaactg gctttccttt tgcactcggg agcgaagaac
     3301 ttttgcctca agcgggtcaa taacgcctgc acgcatatca ccatcaatac tcatttgttt
     3361 tcctggcata ccatcaagag aaaaacgagc actcacttca gcaacacgct cagccccttt
     3421 tgtaataacc ataaattgta caatggtgat aatggtaaaa atgaccaggc caacaacaat
     3481 attaccgcca acaacaaagt taccgaagga gtaaataatg tcacccgcat cgtgatgcag
     3541 cagaataagt ctggttgtac taacactgag cgacaggcgc atcaacgtcg taatcaacag
     3601 gattgtagga aaagaagtta gctcaagagg attttttata tagattgaga gcattaataa
     3661 aagcaatgct gtcgatatat taatcgcgat aatgatatcc accacaactg ttggcaatgg
     3721 aataatcatc atcattacag ccataaagaa gaagagagcc agaataagat cgtgatatga
     3781 ctctgctttt ttaaatatat ttaagagttt attcatgatg tcatcctgcg aactcgttca
     3841 ataatctgca catgctgctg aaaagtattc attagagaaa acacatcaat attggttaac
     3901 aaacaactta agcacaattt gttatcgtag acaaaaatat gttcaatcac tccctgcgtc
     3961 cgttcagggg caagggcatt gaaaaaagaa aaaaacgcat tttcgttata ctctaaggca
     4021 aacgtaataa acacattact atcaattatt tcaataaaaa aagaaaaacc attaaaatca
     4081 atttccacgc gattatcaaa caaataaaaa tcattcccta ataaccttaa aaactcttct
     4141 acgtttcttt taattaaaag at
//
LOCUS       DAZGCS010000230         4128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGCS010000230 DAZGCS010000000
VERSION     DAZGCS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.233"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..1695
                     /gene="dpiB"
                     /locus_tag="ACHK01_005000"
     CDS             37..1695
                     /gene="dpiB"
                     /locus_tag="ACHK01_005000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO8099778.1"
                     /translation="MLQLHENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1664..2344
                     /gene="dpiA"
                     /locus_tag="ACHK01_005001"
                     /gene_synonym="citB"
     CDS             1664..2344
                     /gene="dpiA"
                     /locus_tag="ACHK01_005001"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO8099779.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2385..3770)
                     /gene="dcuC"
                     /locus_tag="ACHK01_005002"
     CDS             complement(2385..3770)
                     /gene="dcuC"
                     /locus_tag="ACHK01_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8099780.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(3909..>4128)
                     /locus_tag="ACHK01_005003"
     CDS             complement(3909..>4128)
                     /locus_tag="ACHK01_005003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099781.1"
                     /translation="EKLFYFLTRYNNHLNEVYNINRQPMLMLDYFLSAQPQLTPLQLT
                     NLHQSTINLHTLLCDNCQPLNMRFVIYV"
BASE COUNT         1138 a          959 c         1058 g          973 t
ORIGIN      
        1 cgtcaaagag gctcgggaca acccgcaagg aaaacaatgt tgcagcttca cgagaataaa
       61 cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct gattctggtg
      121 ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt tgaggactat
      181 ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc ctccaatgac
      241 agtgtcatct ctgcggtgaa aacgcgtgac tacaaacggc tggcgaccat cgctaacaaa
      301 ttacaaagag ataccgattt tgattatgtg gtgattgggg accggcactc gatccgcctt
      361 taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca gggcgcgctg
      421 gagaaagggg agagctactt cattaccggg aaagggtcaa tggggatggc gatgcgcgcc
      481 aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat tggctacctg
      541 gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg cgtgtttgtc
      601 gtgctgttag ggattctgat gttgctgtcg tggttcctgg cagcgcatat ccgtcggcaa
      661 atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga ggcgctgttt
      721 agttcggttt atgaagggct gattgcggtg gatccgcatg gttacattac cgccatcaat
      781 cgtaacgcaa gaaagatgct ggggctgagc tctcccggac ggcaatggtt gggtaaaccc
      841 attgctgaag tggtcaggcc cgccgatttc tttaccgaac agattgatga aaaacgtcag
      901 gatgtggtgg ctaactttaa cggtctgagc gttattgcca atcgggaagc tattcgttca
      961 ggtgatgatt tgctgggggc cattatcagc tttcgtagta aagacgaaat ttccaccctc
     1021 aatgcgcaac tgacgcaaat aaaacaatac gtcgagagcc ttcgtacatt gcgacacgag
     1081 catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta tgatcgcgtg
     1141 ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag cctgcgtgag
     1201 gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg cgcccgggaa
     1261 ctggggctaa aaatgatcat tgtccccggt agccagcttt cgcaactgcc gccaggactg
     1321 gatagcaccg agtttgcagc cattgtgggc aatttacttg ataacgcctt cgaagccagc
     1381 ctgcgtagcg atgaaggaaa caagatcgtt gaattattcc tcagcgatga aggcgatgat
     1441 gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg agacaaaata
     1501 tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat tgggttgtac
     1561 ttgattgcca gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga taatgatccc
     1621 tgcggtacct tgttttcaat ctatattccg aaagtgaaac ctaatgacag ctccattaac
     1681 cctattgatc gttgaggacg aaacgccgct ggcagagatg catgcggaat atattcgtca
     1741 cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc gaatgatgat
     1801 cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg gcagagggat
     1861 taatttattg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt ttaccactgc
     1921 agccagcgat atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg attatctcat
     1981 taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc agcgtaaaca
     2041 tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt ttaatgccta
     2101 tgctcgcggt gaacctaaag acgaactgcc gaccggcatt gatcccttga cgctaaacgc
     2161 ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg tggcgcaggc
     2221 actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca gccgccatct
     2281 gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat accacagtgg
     2341 gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt gcctgtgacc
     2401 gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac caaaccaaca
     2461 agcaccggta ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc catcccggca
     2521 accgcaacga ctacgccaga aacgggcgaa agggtacggc ccaggtttga cgcctgcagc
     2581 atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt cgggatcatc
     2641 tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc cagcatggtc
     2701 agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt agcgatagaa
     2761 atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc agcggcaacc
     2821 agcagcatca ccacgttagc aaacgcatct gccattccgc gataagccac ttccagacca
     2881 gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc aatcagcata
     2941 caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc aaaaatcagt
     3001 acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggagcagt ggtggtgatt
     3061 tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata acgttgccag
     3121 aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag cgtcgttttg
     3181 aaggcgaagt caatcagcga catttcggaa gcttgcgccg ccagcaccac atcccctgaa
     3241 gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc gccacgactg
     3301 ataccaacgt ttaccatcac cggaaatagg gttgccatca gcaaaacacc cagaccggtt
     3361 gcggaagaga cggccagaga catcagacag gcgacaaaat aggcggcaat catcagcagg
     3421 taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat atcattcgcg
     3481 ccgatatggg tcatgtaagc ggcaaatcca cacagcatca taatcatcat gccgaggtcg
     3541 ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc gctgtagcct
     3601 gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat aatcagcaat
     3661 aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat gtagcgagct
     3721 acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat aatttttcct
     3781 gtctccaggc cccaaagtaa ataataaaaa attcctaaag cttaaggaaa aaatatgccc
     3841 aataaattgg cgatgaatgc tgattaaaat caagaaaaac tgccattaag acattgaagt
     3901 tgctgttttt atacatagat aacaaaacgc atatttagcg gttggcaatt atcacataat
     3961 aatgtgtgta aattgatggt ggattgatgc aaatttgtta attgcaatgg tgttagctgc
     4021 ggctgtgcgc tcaaaaaata atctaacatg agcataggtt gacgatttat attatatact
     4081 tcgttaagat gattgttgta tctagttaaa aaataaaata atttttcc
//
LOCUS       DAZGCS010000231         4057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGCS010000231 DAZGCS010000000
VERSION     DAZGCS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.234"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..762
                     /locus_tag="ACHK01_005004"
     CDS             154..762
                     /locus_tag="ACHK01_005004"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO8099782.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            860..2251
                     /gene="selA"
                     /locus_tag="ACHK01_005005"
     CDS             860..2251
                     /gene="selA"
                     /locus_tag="ACHK01_005005"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO8099783.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2248..>4057
                     /gene="selB"
                     /locus_tag="ACHK01_005006"
     CDS             2248..>4057
                     /gene="selB"
                     /locus_tag="ACHK01_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO8099784.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          909 a         1029 c         1204 g          915 t
ORIGIN      
        1 tgagatatcc atcagataga aacaattagg aaaatattta tatcaccatg ataaatatgt
       61 gatttatttg gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca
      121 tactgatatt tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc
      181 ccgtttgtac gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt
      241 aatgaactgc cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg
      301 ccggtgctgg tgaccgaaga gggcgaatgt tggtttgatt cgccgatcat cgctgaatat
      361 attgaattaa tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg
      421 gtgcgcaaaa ttgaggcact ggcggatggc attatggatg ccgggctggt atcggtgcgt
      481 gaacaggcgc gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa
      541 atcaaccgca gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat
      601 acggtcaatc tggcgactat cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc
      661 gttgcgccag gttggtgtgt cgaccgccca cacttagtca aactggtcga aaacctgttt
      721 agccgcgaaa gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct
      781 gacccatctc acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc
      841 ttgatcagcc aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc
      901 tattgatcgc ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac
      961 ccgcgtggtg gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag
     1021 ccagacgctg cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa
     1081 agaagcgcag agcgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa
     1141 ccttggacga gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc
     1201 gccagtgacc ctcgagtacg atctggacga cgccggacgc ggacatcgcg atcgggcgct
     1261 ggcgcaactg ctgtgccgca ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc
     1321 ggcggcggtg ttattgatgt tggcggccac tgccagcggg aaagaggtgg tggtttctcg
     1381 cggcgaactg gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg
     1441 ctgcacccta cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc
     1501 ggtgaatgaa aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg
     1561 gttcaccaaa gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc
     1621 cgtagtgact gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa
     1681 agagccaatg ccgcaggagt tgattgcggc gggcgtcagt ctggtgagtt tctccggcga
     1741 caagttgtta ggcgggccgc aggcaggaat tattgttggt aaaaaagaga tgatcgcccg
     1801 cctgcaaagc cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct
     1861 ggaagccacg ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct
     1921 gcgcctgctt acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggcccc
     1981 ccttgccgca cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat
     2041 tggcagtggt tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca
     2101 tgatggacgc ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc
     2161 ggtgattggt cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga
     2221 gcaacggttt ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac
     2281 ggcaaaacaa ccttattgca ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa
     2341 aaaaagcgcg gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc
     2401 gtgcctggtt ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc
     2461 gttggtggta tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcacag
     2521 acccgtgagc atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg
     2581 accaaagccg atcgcgtgga cgaagcgcgt gttaatgagg ttgaacgcca ggtaaaggag
     2641 gtgctgcggg aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt
     2701 cggggaattg atgccctgcg cgagcatctg cttcagttgc cggaacgcga gcacgccagc
     2761 caacatagtt tccgcctcgc tattgaccgc gcatttaccg taaaaggtgc cgggctggtc
     2821 gtcaccggta cggcgttaag cggggaagtg aaggtaggcg attcactctg gctgactggt
     2881 gtaaataaac cgatgcgtgt acgtgcgctg catgcgcaaa accagccaac agaaaccgcc
     2941 aatgccgggc agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac
     3001 cgtggcgact ggctgcttgc cgatgtgccg ccagagccgt tcacacgggt gattgtcgag
     3061 cttcaaaccc atacaccgct gacccagtgg cagccgctgc atattcacca cgccgccagc
     3121 cacgttacgg gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg
     3181 ccgctgtggc tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg
     3241 ctggcggggg cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa
     3301 tatctgcaat ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt
     3361 catctggaac gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc
     3421 gaagggatgc gcgaattgct gcaacagcct ggctatattc aggctggtta tagcttgttg
     3481 aatgcgccgg ttgccgctcg ctggcaacgg aaaattctcg atacattagc gacttatcat
     3541 gagcaacatc gcgatgaacc tgggcctggg cgcgaacgtc tgcgacgtat ggcgttgcca
     3601 atggaagatg aagcgctagt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc
     3661 ctcagtcatc acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag
     3721 caggccatct ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat
     3781 ctggcaaaag agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg
     3841 cagcaaggaa taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc
     3901 gaatttgcca atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat
     3961 ttccgcgatc gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac
     4021 cgcattggct ttacgcgtcg tcgtggaaat gatcatt
//
LOCUS       DAZGCS010000232         3961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGCS010000232 DAZGCS010000000
VERSION     DAZGCS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.235"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..643
                     /locus_tag="ACHK01_005007"
     CDS             92..643
                     /locus_tag="ACHK01_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8099785.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            703..3207
                     /locus_tag="ACHK01_005008"
     CDS             703..3207
                     /locus_tag="ACHK01_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8099786.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3223..>3961
                     /locus_tag="ACHK01_005009"
     CDS             3223..>3961
                     /locus_tag="ACHK01_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8099787.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCQTVNNSCNVSVAK"
BASE COUNT         1242 a          824 c          800 g         1095 t
ORIGIN      
        1 aaagtaaaat ataatatttg aatgattagc aatccttaaa taacagttac taatcattca
       61 ttttctactt atatttatat aaaggaatat tatgtctgct tttaaaaagt cacttcttgt
      121 tgctggcgta gcaatgattc tttccaataa cgtatttgct gacgaaggtc acggcattgt
      181 taaatttaaa ggtgaagtta tctctgctcc atgttcaatt aaaccaggtg acgaagactt
      241 aactgtaaat ctgggtgaag tagcagatac cgttctgaaa agcgaccaga aatctttggc
      301 tgagcctttc actattcacc tgcaagactg catgttgagc cagggtggaa ccacttattc
      361 aaaagcgaag gttactttca ccacagcgaa tactatgacg ggtcagactg acctgctgaa
      421 aaacaccaaa gaaactgaaa tcggtggtgc cacaggcgtt ggcgtacgta ttctggatag
      481 ccagagcggc gaagtgactt taggtactcc ggtagtaatt acattcaaca atactaacag
      541 ctatcaggaa ctgaacttca aagctcgtat ggaatcccca agcaaagatg ctaccccggg
      601 caacgtttac gctcaggctg actataagat tgcttacgag taatcacatt aggccgggtt
      661 cgcccggcca catcattaac ggattaatga taagtggaac agatgtataa aaaattaaaa
      721 ttaaccacaa taagcgaatt gattaaaaat atttattgtt cattatccgt tctcatcatt
      781 ggttgtgcgt cagcttatgc cgttgaattc aacaaagatt taatcgaagc cgaagatcgt
      841 gaaaacgtta acctttccca atttgaaact gatggccaat tacccgtagg taaatactca
      901 ttaaacgctc ttattaataa taaaaggacg ccaatccatc tggacctgca atgggtatta
      961 attgataacc aaactgcagt ttgtctgact ccagagcaat taacattatt aggctttact
     1021 gatgagatta ttgaagtggc tcagcaaaac ctgatcgatg gttgttaccc tatcgaaaaa
     1081 gaaaaacaaa ttacaactta tctcgataaa gggaaaatgc aattatccat atctgcacct
     1141 caggcatggt taaaatacaa agatgcaaac tggacgcctc ctgaactttg ggatcatggt
     1201 attgctgggg catttcttga ctacaattta tatgcctctc attatgcacc acatcagggc
     1261 gataattcac aaaatataag ttcctatggg caggctgggg ttaatcttgg ggcctggcgc
     1321 ctgcgtactg attaccagta cgatcagtca tttaacaatg gcaaaagcca ggcgaacaac
     1381 ctggattttc cgcgtattta tttgtttcgc ccaatcccag caattaatgc aaaactaact
     1441 ataggtcaat acgatactga atcctctatt ttcgactctt tccatttttc tggcgtttcg
     1501 ttgaaaagcg atgagaatat gttgccgcct gatctacgtg gttacgcacc gcaaatcacg
     1561 ggtgttgcac aaacgaatgc aaaggtcact gtctcacaga acaaccgtat tatttatcaa
     1621 gaaaatgttc ctcctggccc atttgctatt actaatttat tcaatacatt acaggggcaa
     1681 ctggacgtca aggttgaaga agaggacggg caagtcacgc aatggcaagt tgcatctaat
     1741 agtattcctt atctgacgcg taaagggcag attcgctaca ccactgctat gggtaaaccg
     1801 accagcgttg gtggtgattc cttacaacaa cccttcttct ggactggtga attctcatgg
     1861 ggttggctga acaatgtatc cctgtatggt ggttcagttt taacaaaccg tgattatcaa
     1921 tctctggctg ccggcgttgg ctttaacctt aactcattgg gttcattatc ttttgatgtc
     1981 acacgatctg atgctcagtt gcataatcag gataaagaaa cgggttatag ctaccgcgct
     2041 aactattcaa aacgttttga atctaccggt agccagctca ctttcgctgg ttaccgtttc
     2101 tctgataaaa actttgtgac aatgaatgaa tatatcaatg acactaacca ttacacgaat
     2161 tatcagaatg aaaaagagag ttatattgtc acgtttaacc agtatcttga atcattaagg
     2221 ttaaatacat acgtaagttt ggctcgtaat acttactggg acgccagcag taatgtgaat
     2281 tattcattat cacttagccg cgattttgat atcgggccat taaaaaacgt ctccacttct
     2341 ctaacattta gccgaataaa ctgggaagaa gacaaccagg atcaactgta cctaaatatt
     2401 tcgattccct ggggaaccag tagaacattg agctatggta tgcaacgaaa tcaggataac
     2461 aagatttcgc atactgcttc gtggtatgac tcttccgatc gaaataattc ctggagcgtt
     2521 tctgcttcag gcgacaatga tgaattcaaa gatatgaaag cgtcactacg cgccagttat
     2581 cagcataata ccgagaacgg tcgtctctat ctctccggga catcacagcg agacagttat
     2641 tattctctga atgccagttg gaatggttca ttcacggcga ctcgtcacgg agccgctttc
     2701 catgactata gtggtagtgc tgactcgcgt tttatgatcg acgcagatgg tgcagaagat
     2761 attccgttga acaataaacg cgcggtaact aatcggtatg gcatcggagt tattccatca
     2821 gtcagcagtt acataacaac atcattaagc gttgataccc gaaatcttcc agaaaatgtg
     2881 gatatcgaaa actcggttat caccaccacc ttaaccgaag gtgctattgg ctacgccaaa
     2941 cttgataccc gcaagggcta tcaaatcatg ggggttattc gcctggcaga tggtagtcac
     3001 ccaccactgg ggattagcgt aaaagataaa accagccata aagaattagg actggttgct
     3061 gatggcggct ttgtatacct caacggcatt caggatgaca gcaaacttac tttacgctgg
     3121 ggtgataaat cctgttttat tcaaccaccc aatagcagca acttaaccac cggaacggtt
     3181 attttaccgt gtattagcca aaattaattc aggatgaaac taatgcgcta cttaaacaca
     3241 aagaatctca ttgcagcagg agtattactc gcctgcatga gcagcattgc ctggggtgcc
     3301 atcatcccgg accgtacccg tatcataatg aatgagtcag ataaagggga agcattaaaa
     3361 cttactaatc aaagcaaaaa tctaccctat ctggcgcaaa catggataga agatacgaaa
     3421 ggcaataaat cacgtgactt tattgtcacg gttcccccta tggtacgttt aaatcccagt
     3481 gagcaaattc aaataagaat gataagtcag gaaaaaattg ctcaactgcc taatgacaga
     3541 gaaacgctct tctattttaa cgtgcgagaa ataccgccaa aaacagataa aaaaaatgtg
     3601 atgcaagtca ctatgcagca cgcattgaaa ctattctggc gaccaaaagc tattgaatta
     3661 gaagatgatg gggtaatgtc ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg
     3721 atccgcttta acaataagat gccttatcac gttacgctgg gttatatcgg aacaaacgga
     3781 atcacaatgt tgccacaaac gcaaagttta atggtaaccc cttatagcca tgcagatacg
     3841 cagtttaaaa atgttccgtc agcttttcag gttggttata tcaatgattt tggtggatta
     3901 agtttttatg aaataaactg ccagacggtt aataactctt gtaatgtttc cgtagccaaa
     3961 c
//
LOCUS       DAZGCS010000233         3951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGCS010000233 DAZGCS010000000
VERSION     DAZGCS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.236"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            823..1065
                     /locus_tag="ACHK01_005010"
     CDS             823..1065
                     /locus_tag="ACHK01_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO8099788.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1097..1747)
                     /gene="tetR(A)"
                     /locus_tag="ACHK01_005011"
     CDS             complement(1097..1747)
                     /gene="tetR(A)"
                     /locus_tag="ACHK01_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO8099789.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1853..3052
                     /gene="tet(A)"
                     /locus_tag="ACHK01_005012"
     CDS             1853..3052
                     /gene="tet(A)"
                     /locus_tag="ACHK01_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO8099790.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3084..>3951)
                     /locus_tag="ACHK01_005013"
     CDS             complement(3084..>3951)
                     /locus_tag="ACHK01_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO8099791.1"
                     /translation="DLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLV
                     MIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISA
                     ALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKW
                     QPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLW
                     FRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNR
                     TPRARIACRKSP"
BASE COUNT          759 a         1240 c         1214 g          738 t
ORIGIN      
        1 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
       61 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
      121 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
      181 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
      241 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
      301 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
      361 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
      421 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
      481 gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg
      541 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
      601 cccggtgagc gtcggaaagg cgctggaagc cccgtagcga cgcggagagg ggcgagacaa
      661 gccaagggcg caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt
      721 tccctgcggt gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc
      781 ttgagtccac gctagatcta tctcatctgc gcaaggcaga acgtgaagac ggccgccctg
      841 gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac
      901 gagcggacgc cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag
      961 gcttggacgc tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg
     1021 cggtggaaag ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct
     1081 ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga
     1141 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
     1201 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
     1261 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
     1321 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
     1381 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
     1441 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
     1501 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
     1561 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
     1621 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
     1681 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
     1741 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
     1801 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
     1861 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
     1921 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
     1981 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
     2041 gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc
     2101 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
     2161 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
     2221 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     2281 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     2341 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     2401 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     2461 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     2521 acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg
     2581 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     2641 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     2701 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     2761 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     2821 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     2881 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     2941 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     3001 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     3061 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     3121 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     3181 tgaagcgcac tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg
     3241 ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa
     3301 aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt
     3361 cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct
     3421 ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg
     3481 gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca
     3541 ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata
     3601 gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg
     3661 accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc
     3721 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
     3781 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
     3841 aacgcccgca gcatcgcgac cgtcatcggt gagaagttcg gcaggtattg ggtggtgaca
     3901 atgtaggtgc tgccccaaat ggcaggtgct atcgctgtga acaataaatc g
//
LOCUS       DAZGCS010000234         3842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGCS010000234 DAZGCS010000000
VERSION     DAZGCS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.237"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..2427
                     /gene="ydeP"
                     /locus_tag="ACHK01_005014"
     CDS             148..2427
                     /gene="ydeP"
                     /locus_tag="ACHK01_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO8099792.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2675..2872
                     /gene="safA"
                     /locus_tag="ACHK01_005015"
     CDS             2675..2872
                     /gene="safA"
                     /locus_tag="ACHK01_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO8099793.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2947..3708
                     /gene="ydeO"
                     /locus_tag="ACHK01_005016"
     CDS             2947..3708
                     /gene="ydeO"
                     /locus_tag="ACHK01_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO8099794.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
BASE COUNT         1089 a          846 c          873 g         1034 t
ORIGIN      
        1 atctattgtt taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat
       61 atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa
      121 taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct
      181 gcaggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata
      241 cgccaggatg ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt
      301 tgtgcatggc cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa
      361 gcaatcgcct gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg
      421 gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag
      481 cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc
      541 gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact
      601 tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg
      661 agcaataact ttcccgactg ctccaacatg tgccatgaac cgaccagtgt gggtttggca
      721 gcgagtatcg gcgtgggtaa agggactgtg ttgctggaag actttgagaa gtgcgattta
      781 gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc
      841 gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg
      901 gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg
      961 gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg
     1021 cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat
     1081 gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat
     1141 tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg
     1201 gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag
     1261 cacgaacatg gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac
     1321 attggcaagc ctggtgcggg tatctgccca ctacgtggcc actctaatgt acagggcgac
     1381 cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc
     1441 tatggcttca caccacctca agcacctgga catgctgcaa ttgccagcat gcaagcaata
     1501 tgtacagggc aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca
     1561 gatcgggaag cgagcgctgt accgttaacg caattagatc tggcggtaca cgtagccact
     1621 aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga
     1681 cgtagcgaga ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg
     1741 tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag
     1801 tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag
     1861 tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc
     1921 gccgactata accaacgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct
     1981 gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta
     2041 gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat
     2101 cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga
     2161 gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga
     2221 gttaatctta ttgcgctcac gccagacggt aaacgtagct cacgccgcat ggatagatta
     2281 aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat
     2341 cacatgctaa cactcgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt
     2401 ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca
     2461 cagcagaaga atccctcatt gactattatc gcgcaatttg ctcacatgga ttaaattaaa
     2521 ctacatacta taagatataa acctctgcct acagctgtaa gaaactccgc tcagtactga
     2581 agtgccagtc ctatttcctc ttttctccag cctgttatat taagcatact gattaacgat
     2641 ttttaacgtt atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa
     2701 aatcacgcaa agagacaact ataaagaaat catgtctgta attgtggttg tcttattact
     2761 gacccttacg ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg
     2821 tcgcattgca agagatctta cacatttcat tatcaatagt ttgcaagact gataataaac
     2881 agaaaattat aaatctgaaa tgttaacaaa gtatcgataa aaactttatt gttttaagga
     2941 gataaaatgt cgctcgtttg ttctgtcata tttattcatc atgctttcaa cgctaacatt
     3001 ttagataaag attacgcctt ctctgacggc gagatcctga tggtagataa cgctgttcgt
     3061 acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga aaataccatt
     3121 aaaaaatatc tacaatgcac taacatccag acagtgacga tgcctgttcc tgcgaagttt
     3181 ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg
     3241 gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt
     3301 gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt ttccgggaaa
     3361 gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac
     3421 tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc
     3481 tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt
     3541 tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg
     3601 ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagt
     3661 cacatgggta tgaatacggg caacacgatg aatgctttag ctatttgatt atttgctaac
     3721 gagtagtcaa ccacgcaccc tacgtaaaaa ttaaaaatgg ggcagtcatt ccctgccccg
     3781 cgttgttttt aggtgatata tttattgaaa taaataagtg acattcatca catatttatg
     3841 ca
//
LOCUS       DAZGCS010000235         3605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGCS010000235 DAZGCS010000000
VERSION     DAZGCS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.238"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..245
                     /locus_tag="ACHK01_005017"
     CDS             <1..245
                     /locus_tag="ACHK01_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO8099795.1"
                     /translation="PQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQEAVDEI
                     ESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            242..436
                     /locus_tag="ACHK01_005018"
     CDS             242..436
                     /locus_tag="ACHK01_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO8099796.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            429..863
                     /locus_tag="ACHK01_005019"
     CDS             429..863
                     /locus_tag="ACHK01_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8099797.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1370..2317
                     /gene="stxA1a"
                     /locus_tag="ACHK01_005020"
     CDS             1370..2317
                     /gene="stxA1a"
                     /locus_tag="ACHK01_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO8099798.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2327..2596
                     /gene="stxB1a"
                     /locus_tag="ACHK01_005021"
     CDS             2327..2596
                     /gene="stxB1a"
                     /locus_tag="ACHK01_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO8099799.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            3096..>3605
                     /locus_tag="ACHK01_005022"
     CDS             3096..>3605
                     /locus_tag="ACHK01_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8099800.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT          965 a          723 c          941 g          976 t
ORIGIN      
        1 cacctcaact ccgatttgat gaacgcaata ttcacaagca atgcgtggtg tgcaaccagc
       61 acaaaagcgg aaatctcgtt ccgtatcgcg tcgaactgat taaccgcatc gggcaggaag
      121 cagtagacga aatcgaatcg aaccataacc gccatcgctg gactgtcgaa gaatgcaggg
      181 cgatcaaggc gaagtatcag cagaaactta aagacctgcg aaacagcaga agtgaggccg
      241 catgacgttc acagtaaaaa ccattcctga catgctcctt gaggcatatg gaaatcagac
      301 cgaggtagcc cgaatactga actgcaatcg tgccacagtc agaaaataca ttggcgataa
      361 agaagggaaa aagcacgccg tcgtcaatgg cgtccttatg gttcatcgcg gatggggtaa
      421 agatactgat gcgtgatatc cggcaggttc ttgagcgctg gggggcatgg gcggcaaata
      481 accatgagga tgttacatgg tcgcccattg ctgccggatt taagggactg atccccgaaa
      541 aagtaaaatc acgtccacag tgttgtgacg atgacgcgat gattatatgc gggtgtatgg
      601 ctcgccttaa caggaacaac agcgatctgc atgacttgct ggttgattat tacgtgttgg
      661 gggagacgtt catggcgctg gcacggaaac atgggtgctc tgacacctgt ataggtaaac
      721 gccttcacaa agcggagggg attgttgaag gcatgctgat gatgctggga gtgaggcttg
      781 agatggatcg gtatgttgag cgtgaattgc cgggagggag aacctctgta ttttatcagc
      841 gaaaaaatag tttacgatcg taaaaatctg catatcatga taagagtggt tacattgcca
      901 cgctgcttaa cccgccgatg cgcgggtttt tttgtaccca gaatcctgtg agctatacgg
      961 aaagtacaca gaaaggaagg tgcgaccaca attaataaca aaatcttaaa aattgcacat
     1021 ggcactatta gttttctaaa tattgtgtat tttttgtatt gcaggatgac cctgtaacga
     1081 agtttgcgta acagcatttt gctctacgag tttgccagcc tcccccagtg gctggctttt
     1141 ttatgtccgt aacatcctgt gtatcaataa atgttgttat ctacgtacgt caagtagtcg
     1201 catgagatct gaccagatat gttaaggttg cagctctctt tgaatatgat tatcattttc
     1261 attacgttat tgttacgttt atccggtgcg ccgtaaaacg ccgtccttca gggcgtggag
     1321 gatgtcaaga atatagttat cgtatggtgc tcaaggagta ttgtgtaata tgaaaataat
     1381 tatttttaga gtgctaactt ttttctttgt tatcttttca gttaatgtgg tggcgaagga
     1441 atttacctta gacttctcga ctgcaaagac gtatgtagat tcgctgaatg tcattcgctc
     1501 tgcaataggt actccattac agactatttc atcaggaggt acgtctttac tgatgattga
     1561 tagtggcaca ggggataatt tgtttgcagt tgatgtcaga gggatagatc cagaggaagg
     1621 gcggtttaat aatctacggc ttattgttga acgaaataat ttatatgtga caggatttgt
     1681 taacaggaca aataatgttt tttatcgctt tgctgatttt tcacatgtta cctttccagg
     1741 tacaacagcg gttacattgt ctggtgacag tagctatacc acgttacagc gtgttgcagg
     1801 gatcagtcgt acggggatgc agataaatcg ccattcgttg actacttctt atctggattt
     1861 aatgtcgcat agtggaacct cactgacgca gtctgtggca agagcgatgt tacggtttgt
     1921 tactgtgaca gctgaagctt tacgttttcg gcaaatacag aggggatttc gtacaacact
     1981 ggatgatctc agtgggcgtt cttatgtaat gactgctgaa gatgttgatc ttacattgaa
     2041 ctggggaagg ttgagtagcg tcctgcctga ctatcatgga caagactctg ttcgtgtagg
     2101 aagaatttct tttggaagca ttaatgcaat tctgggaagc gtggcattaa tactgaattg
     2161 tcatcatcat gcatcgcgag ttgccagaat ggcatctgat gagtttcctt ctatgtgtcc
     2221 ggcagatgga agagtccgtg ggattacgca caataaaata ttgtgggatt catccactct
     2281 gggggcaatt ctgatgcgca gaactattag cagttgaggg ggtaaaatga aaaaaacatt
     2341 attaatagct gcatcgcttt catttttttc agcaagtgcg ctggcgacgc ctgattgtgt
     2401 aactggaaag gtggagtata caaaatataa tgatgacgat acctttacag ttaaagtggg
     2461 tgataaagaa ttatttacca acagatggaa tcttcagtct cttcttctca gtgcgcaaat
     2521 tacggggatg actgtaacca ttaaaactaa tgcctgtcat aatggagggg gattcagcga
     2581 agttattttt cgttgactca gaatagctca gtgaaaatag caggcggaga ttcataaatg
     2641 ttaaatacat ctcaattcag tcagttgttg ccggtctgat aatagatgtg ttagaaaatt
     2701 tctgcatggt gaatccccct gtgcggaggg gcgactggtg aacggtatga tctctttgat
     2761 gatcgtaagc gagaatacgc gggtttggtg gcaccaggcc gaactcaccg ggaggcaccc
     2821 ggcatcatgc tgtatacaga gattaggcat atatccaggc tcctcatcgc aggagccttt
     2881 ttacatgcaa aaaaaagccc gagtgggttc gggcaacagc atgagatact tgcattgtca
     2941 tttttatcgt gcggatttta accaggattc ataaggctgc gcaactgcgc ggcctttttc
     3001 gtatttcggg ctgtagtccc tgtgtgtcat tcaggcttcc ggactacagc ccactccata
     3061 tctgatttaa tacactatcc cggccgggag gaataatgac atttaaacat tatgatgttg
     3121 tcagggcggc gtcgccgtca gaccttgcgg aaaagctgac acacaaactg aaagagggct
     3181 ggcagccgtt tggtagtccg gtggccataa ccccttatac cctgatgcag gccattgcgg
     3241 cggaaggtga tgtcaccaca ccagtggcgg tgaccggtaa tgagggtaag gcggtggctg
     3301 tcagtgccac cagcgacccg gagtattact ttgttgtggt tctggcaggg cagtcaaatg
     3361 gcatgtcgta tggtgaaggt cttccgctgc cggagacata tgaccgtccg gacccgcgta
     3421 ttaagcagct ggcgcgccgc agtacggtga caccgggcgg tgcagcatgc aaatataacg
     3481 acatcattcc ggcggaccat tgtctgcatg atgtgcagga catgagccgt cttaaccatc
     3541 cgaaagcgga cctgtcaaaa gggcagtacg gtaccgtggg gcaggggctg catatcgcca
     3601 aaaaa
//
LOCUS       DAZGCS010000236         3554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGCS010000236 DAZGCS010000000
VERSION     DAZGCS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.239"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..785
                     /locus_tag="ACHK01_005023"
     CDS             <1..785
                     /locus_tag="ACHK01_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO8099801.1"
                     /translation="ALISAQGVNEIISKIGADAVALPENFRIHDLEKFNLNRFRFRGA
                     LSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKA
                     APFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQ
                     TADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRP
                     VLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            914..1450
                     /gene="yfdR"
                     /locus_tag="ACHK01_005024"
     CDS             914..1450
                     /gene="yfdR"
                     /locus_tag="ACHK01_005024"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8099802.1"
                     /translation="MSFIKTFSGKHFYYDRINKDNIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            1441..1803
                     /locus_tag="ACHK01_005025"
     CDS             1441..1803
                     /locus_tag="ACHK01_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099803.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LESKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPETKK"
     gene            1800..2015
                     /locus_tag="ACHK01_005026"
     CDS             1800..2015
                     /locus_tag="ACHK01_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099804.1"
                     /translation="MSVIKTHTGIVITRDGEKRMKLHSTETSWVAGRCESYDKKTGYR
                     WGAPNMRRRLLLDSIRPIKQVATREQN"
     gene            2075..2281
                     /locus_tag="ACHK01_005027"
     CDS             2075..2281
                     /locus_tag="ACHK01_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8099805.1"
                     /translation="MLNLDCVPISTYCKETGETPEAINKRVQRGVWREGVQVLKVEGV
                     KERWIDLSEVAKWARQNCSNYRAA"
     gene            2242..3417
                     /locus_tag="ACHK01_005028"
     CDS             2242..3417
                     /locus_tag="ACHK01_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO8099806.1"
                     /translation="MGQTKLLKLPRGVTIRKHRQGETINITFTYKGVRCREPLSNLEV
                     TPKNIKYAERTLGEIHNKIERGTFIYAEYFPRSARLKIFGNAAAGKTVKMYLDEYLEI
                     CETRKLSPSTIGGYKKCRSALASLHICPASELTPATLKAWIQSQKTTLKTIRNQLSFL
                     RSALDEAVTDGVLQINPVSLVTASRYQSDKSEAESSYVVDPLSPAEVDALLAAAGNKQ
                     WENLFRFAIHTGLRSSELCALRWRDIDFVGKTAHVQSASVVGVIKGTKTKAGTRKVEL
                     TEEAMLALINQKPFTFMKDATVFEDPKTNKPWASADAIRKKAWVPTLRKAGIRYRNPY
                     QTRHTYATKYISQGANLFWLAGQMGHKGPEMLFRHYGRYLSDYDNHSSTTLTSNCKK"
BASE COUNT         1055 a          778 c          842 g          879 t
ORIGIN      
        1 atgcccttat ttctgctcag ggtgttaatg aaattatcag taagattggt gccgacgcag
       61 tggcattacc tgaaaatttc cgcattcatg atctggaaaa atttaattta aatcgcttcc
      121 gtttccgtgg tgcgctttcc actgccagca tcgatgactt tacccgttat tctaaagatc
      181 ttgcagatga aggcacccgc tgctttatcg atgctgataa tatgcgtgcc gtcagtgtgc
      241 ttaacctggg tactattgat gaaccaggtc acgcagataa caccgccact ctcaaactga
      301 aaaaggcagc gccgttctct gctctgttgt ctgttaatgg cgagcgtaac tcccagaagt
      361 cactggcaga atggattgaa gactgggccg actaccttgt gggctttgat gctaatggtg
      421 acaccattca ggcaacaaaa gcggctgcgg cggtccgtaa aatcacgatt gaagcaaacc
      481 agactgctga ttttgaagat aatgacttca gcggcaaacg ctccctgatg gagtctgtcg
      541 aagcgaagac caaagacatt atgccagtgg catttgagtt taaatgcgtt ccgtttgaag
      601 gtctgaaaga acgtccgttt aaattacgcc tcagcattat cactggcgat cgtccggtac
      661 tggttctacg cattattcag ctggaagcgg tgcaggaaga tatggctaac gaatttcgtg
      721 atctgcttgt tgagaaattc aaggacagca aagtagaaac ctttattggg actttcaccg
      781 cctgatttca ttaccgcaaa tgcccctgcg ggggcattta tggaaacgta attgactcaa
      841 taatcgcctg aaggcgaggg ttttctttaa ccaaaattca gcgcggtgca gcgcatataa
      901 cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt attatgacag
      961 gataaataaa gacaacatcg atattaacga tatcgcggtt tccctttcaa atatctgtcg
     1021 ctttgccggc catctttcac acttctacag cgtcgcccaa catgcggtgc tttgcagcca
     1081 gctggtaccg caggaatttg cttttgaagc gttaatgcat gatgcaacag aagcgtattg
     1141 ccaggacatc cccgcaccac tgaaacgcct tcttcctgac tataaacgga tggaagaaaa
     1201 aatagacgcc gtaatccgtg agaaatacgg gttacccccg gttatgagca cgcctgtgaa
     1261 atatgccgat ctcatcatgc tggcaaccga acgccgcgat ctcgggcttg atgatggctc
     1321 tttctggcct gtactggaag gcatcccggc aacagagatg ttcaacgtga ttccactggc
     1381 accgggccat gcctacggga tgtttatgga acgttttaac gagttatcgg agttacgcaa
     1441 atgcgcatga atgttttcga aatggaaggg tttcttcgtg ggagatgtgt accgcgagat
     1501 ctgaaagtga acgaaacaaa tgctgagtac ctggtacgta aattcgacgc gcttgaagct
     1561 aaatgtgcgg cactggaaag caaaataata ccagtgtcag ctgaactgcc tccagcaaat
     1621 gaaagtgttc tgttatttga tgctaacgga gaaggctggc tgattggctg gcgttctctc
     1681 tggtacacct ggggacaaaa agaaaccgga gaatggcagt ggacatttca ggtcggggac
     1741 cttgaaaacg tcaatatcac tcactgggca gtaatgccga aagcaccaga gactaagaaa
     1801 tgagcgtgat aaaaactcat acaggaattg ttatcacccg agacggtgaa aagcggatga
     1861 aattacattc cactgaaacg tcctgggttg ccggacgttg tgaatcctac gacaaaaaga
     1921 ctggttaccg ttggggtgca cctaacatgc gtcgccgtct gctactggac agcatcaggc
     1981 caataaaaca agtagcaacc agagaacaaa attaattatc aggactggaa tttgatatta
     2041 ctgcccatgt gcagcgggct aagtggagaa acatatgctg aacctcgatt gtgttcctat
     2101 ctcaacttat tgcaaagaaa ctggcgaaac tcctgaagca ataaacaaac gtgtacagcg
     2161 cggtgtttgg cgtgaaggtg ttcaggtttt aaaggttgaa ggcgttaagg agaggtggat
     2221 tgatcttagt gaggttgcaa aatgggccag acaaaactgc tcaaactacc gcgcggcgta
     2281 acaatcagga aacaccgcca gggcgaaacg atcaatataa ctttcaccta caaaggagtt
     2341 agatgtcgtg agccgctttc caatctggaa gtaacaccaa agaacattaa atacgccgag
     2401 cgtacactcg gcgaaatcca taataagatc gaaaggggaa cattcattta tgcagaatat
     2461 tttccccgtt ctgctcgttt gaaaattttt ggtaatgctg ctgcaggcaa aacggtaaag
     2521 atgtacctgg acgaatatct tgaaatctgc gaaacaagaa aactttcacc ctctacgatt
     2581 ggtggctata aaaaatgccg tagtgcgtta gcctcacttc acatttgccc tgcaagtgaa
     2641 ttaacaccag caaccctgaa agcgtggatt caaagccaga aaacaacctt aaaaaccatt
     2701 cgcaaccagt tatctttcct gcggtcagca cttgatgaag ccgtgaccga tggggtactt
     2761 caaattaacc ccgtatcgtt agtaacagct tcgcgctacc aaagtgataa gtcagaagca
     2821 gaaagcagct atgtggttga tccgctatca ccagcagaag ttgatgcatt actagcagca
     2881 gccggaaaca aacaatggga aaatctgttc cggttcgcta tacatacagg cctgcgtagt
     2941 tctgaactat gtgcccttcg atggcgtgat atcgactttg ttggaaaaac agcacatgtc
     3001 cagagcgcaa gtgttgttgg tgttatcaaa gggaccaaaa caaaagccgg tactcgtaaa
     3061 gttgaactga cagaagaggc aatgttggcg ctgataaatc agaagccatt tacgttcatg
     3121 aaagatgcta cggtctttga agatccaaag accaataagc cttgggcaag tgctgatgca
     3181 atcaggaaaa aagcatgggt gccaacattg cgaaaagcag gtattcgcta cagaaaccca
     3241 tatcagacca ggcacactta tgctactaag tacattagcc aaggagctaa tctcttctgg
     3301 ctagcagggc agatggggca taaggggccc gaaatgttgt ttcgtcatta tgggcgttac
     3361 ttgagtgatt atgacaacca cagctcaaca acgctcacaa gtaattgcaa aaagtagcct
     3421 tgagcaaggt aaagcatgtt attcaaagaa gcctatttcg ttcaaaacga gaggtcctcc
     3481 acaaatccac gcttgaatag gtattcttta caattatgag catacccctt atagatttat
     3541 tccattgaaa aatg
//
LOCUS       DAZGCS010000237         3553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGCS010000237 DAZGCS010000000
VERSION     DAZGCS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.240"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1572
                     /gene="tssI"
                     /locus_tag="ACHK01_005029"
     CDS             <1..1572
                     /gene="tssI"
                     /locus_tag="ACHK01_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO8099807.1"
                     /translation="SLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSAQIRPS
                     SVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWR
                     NNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGT
                     ALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRY
                     NPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENR
                     TPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDV
                     INNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGV
                     AYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTA
                     IYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPP
                     KTPDEKQDTPDMREY"
     gene            1639..>3553
                     /gene="rhsD"
                     /locus_tag="ACHK01_005030"
     CDS             1639..>3553
                     /gene="rhsD"
                     /locus_tag="ACHK01_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO8099808.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRS"
BASE COUNT          799 a          988 c         1173 g          593 t
ORIGIN      
        1 agcctggtgc tgtgcgacac agtccgccat ctgcccgaat cttttgaaat cccctggaat
       61 ccgaacaccc gtaccgaggt gagcaccctc tgcatcagcc agttccgcta cagcgcacaa
      121 atccgccctt cttccgtggt gaccaaagac tacaccttta aacgccccgg ctgggccgga
      181 cgttttgaac aggaaggcca gcaccaggac taccagcgca cgcagtatga agtgtatgac
      241 taccccggac gtttcaagag cgcccacggg cagaactttg cccgctggca gatggacggc
      301 tggcgaaaca acgcagaaac cgcgcgggga atgagccgct cgccggagat atggccggga
      361 cggcgaattg tgctgacggg gcatccgcag gcgaacctga accgggaatg gcaggtggtg
      421 gcaagtgaac tgcacggcga acagccacag gcggtgccag gacggcaggg agcggggacg
      481 gcgctggaga accattttgc ggtgatcccg gcagacagaa catggcgacc acagccgttg
      541 ctgaaaccgc tggtcgacgg cccgcagagc gctgtcgtga caggaccggc aggcgaggaa
      601 atcttctgcg acgaacatgg tcgcgtgcgg gtgaagttca actgggaccg ttataacccg
      661 gcagaccaag acagttcgtg ctggatccgt gtggcacagg cgtgggcagg caccggtttt
      721 ggccacctgg cgataccgcg tgtgggtcag gaggtgattg tggacttcct caacggcgat
      781 ccggaccagc cgattattat ggggcgtacc taccaccagg aaaaccgcac acccggcagc
      841 ctgccgggga cgaagacgca gatgaccatt cgttcgaaaa cctataaggg cagcgggttt
      901 aatgaactga agtttgacga tgcgacaggg aaagaacagg tctacatcca cgcgcagaag
      961 aacatgaaca ccgaggtgct gaataaccgc accacggatg tgataaacaa ccatgctgag
     1021 accattggca acaatcagat gattgcggtt accaacaatc agatacagac ggtgggcgtt
     1081 aaccagatag agacggtggg cagtaaccag atcattaacg tgggttctgt tcaggtggaa
     1141 acgattggac ttgttcgtgc gctgaccgtg ggcgtggcgt accagacgac ggtaggtggc
     1201 attatgaaca cttcggtggc tctgatgcag tcctcgcaga tcggtttgca taaatcgttg
     1261 agggtcgggc tgggttatga cgtcaaagtc ggaaataacg ttaccttcac cgttggtaaa
     1321 acgaaaaagg atgataccgg gcagaccgcg atttactccg ccggtgagca tctggagctc
     1381 tgctgtggta aggcaaggct ggtgctgacg aaggacggac aaatttttct caacggcaca
     1441 aaaattcatt tgcagggtaa ggagcaggtt aatggtgact cactgttgat taactggaac
     1501 tgtgcagcct cgaaatcccc accgaagacc cctgatgaaa agcaggatac gccggatatg
     1561 agagagtact gatcccgtac ctgttcggta ttcagtaaca aacaagcatt aaaaataaac
     1621 agaagaagga attgaatcat gagcggaaaa ccagcggcgc gtcagggaga tatgactcag
     1681 tatggcggtc ctattgtcca gggttcggca ggtgtaagaa ttggcgcgcc caccggcgtg
     1741 gcgtgctcgg tgtgtccggg cgggatgact tcgggcaacc cggtaaatcc gctgctgggg
     1801 gcgaaggtgc tgcccggcga gacggacctt gcgctgcccg gcccgctgcc gttcattctc
     1861 tcccgcacct acagcagcta ccggaccagg acacccgcgc cggtgggagt gtttggcccc
     1921 ggctggaaag cgccttctga tatccgctta cagctacgcg atgatgcact ggtactcaac
     1981 gacaacggcg ggcggagcat tcactttgag ccgctgctgc cgggggaggc ggtgtacagc
     2041 cgcagcgagt caatgtggct ggtgcgcggt ggtaaggcag cgcagccgga cggccacacg
     2101 ctggcgcggc tgtggggggc gctgccgccg gatatccggt taagcccgca tctttacctg
     2161 gcgaccaaca gcgcacaggg gccgtggtgg atactggggt ggtcagagcg ggtgccgggt
     2221 gctgaggacg tactgccagc gccgctgccg ccgtaccggg tgcttaccgg gctggcagac
     2281 cgtttcgggc ggacgctgac gtaccggcgt gaggccgcag gcgacctggc cggggaaatc
     2341 accggcgtga cggacggtgc cgggcgtgag ttccgtctgg tactgaccac acaggcacag
     2401 cgggcggaag aggcccgcac ctcttcgcta tcttcttctg acagttcccg ccctctctca
     2461 gcctcaccgt tccccgacac actgcccggt accgaatacg gccccgacag gggcatccgt
     2521 ctttcggcgg tgtggctgat gcacgacccg gcatacccgg aaagcctgcc cggtgcgcca
     2581 ctggcgcggt acacgtatac ggaagccggt gaactgctgg cggtatatga ccgcagcaat
     2641 acgcaggtgc gcgctttcac gtatgacgcg cagcacccgg gccggatggt ggcgcaccgt
     2701 tacgcgggaa ggccggagat gcgctaccgc tacgacgata ccgggcgggt ggtggagcag
     2761 ctgaacccgg caggcctgag ttaccgctac cagtatgagc aggaccgcat caccgtcacg
     2821 gacagcctga accggcgtga ggtgctgcat acagaaggcg gggccgggct gaagcgggtg
     2881 gtgaaaaaag aactggcgga cggcagcgtc acgcacagcg ggtatgacgc ggcaggaagg
     2941 ctcacggcgc agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtcc
     3001 ggcgatatca cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac
     3061 gggaaccagc tgacggcggt ggtgtccccg gacgggctgg agagccgccg ggaatatgat
     3121 gaaccgggca ggctggtatc ggagacatcg cgcagcgggg agacagtacg ctaccgctac
     3181 gatgacgcgc acagtgagtt accggcgacg acaacggatg cgacgggcag cacccggcag
     3241 atgacgtgga gccgctacgg gcagttgctg gcgttcactg actgctcggg ctaccagacc
     3301 cgctatgaat acgaccgctt cggccagatg acggcggttc accgcgagga aggtatcagc
     3361 ctttaccgcc actatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt
     3421 gaaacgcagt atgagtacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc
     3481 aaccggagcg agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg
     3541 ctgacgcgca gta
//
LOCUS       DAZGCS010000238         3521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGCS010000238 DAZGCS010000000
VERSION     DAZGCS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.241"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..374
                     /locus_tag="ACHK01_005031"
     CDS             <1..374
                     /locus_tag="ACHK01_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO8099809.1"
                     /translation="DTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTA
                     EEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEED
                     GAATNKKTLADYARALSGEDE"
     gene            371..577
                     /locus_tag="ACHK01_005032"
     CDS             371..577
                     /locus_tag="ACHK01_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO8099810.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            574..2175
                     /locus_tag="ACHK01_005033"
     CDS             574..2175
                     /locus_tag="ACHK01_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8099811.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFVQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            2156..3475
                     /locus_tag="ACHK01_005034"
     CDS             2156..3475
                     /locus_tag="ACHK01_005034"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8099812.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
BASE COUNT          786 a          949 c         1113 g          673 t
ORIGIN      
        1 cggataccgc gaaagagcag atttataacc gcttcacact gacgccggaa ggggatgaac
       61 cgcttcccgg tgccgttcac ttcccgaata acccggatat ttttgatctg accgaagcgc
      121 agcagctgac ggctgaagag caggtcgaaa aatgggtgga tggcaggaaa aaaatactgt
      181 gggacagcaa aaagcgacgc aatgaggcac tcgactgctt cgtttatgcg ctggcggcgc
      241 tgcgcatcag tatttcccgc tggcagctgg atctcagtgc gctgctggcg agcctgcagg
      301 aagaggatgg tgcagcaacc aacaagaaaa cactggcaga ttacgcccgt gccttatccg
      361 gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac tgcatgacct
      421 gatgacaggt aaacgggtgg caacagtaca gaaagacgga cgaagggtgg agtttacggc
      481 cacttccgtg tctgacctga aaaaatatat tgcagagctg gaagtgcaga ccggcatgac
      541 acagcgacgc aggggacctg caggatttta tgtatgaaaa cgcccaccat tcccaccctt
      601 ctggggccgg acggcatgac atcgctgcgc gaatatgccg gttatcacgg cggtggcagc
      661 ggatttggag ggcagttgcg gtcgtggaac ccaccgagtg aaagtgtgga tgcagccctg
      721 ttgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa taacggctat
      781 gccgccaacg ccatccagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt
      841 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
      901 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
      961 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
     1021 gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc
     1081 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
     1141 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
     1201 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
     1261 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
     1321 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagctg
     1381 cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct ggatacgcag
     1441 tcagcgatgg attttattct gggcgcgaac agtcaggagc agcgggaaag gctgaccggc
     1501 tggattggtg aaattgccgc gtattacgcc gcagcgccgg tccggctggg aggcgcaaaa
     1561 gtaccgcacc tgatgccggg tgactcactg aacctgcaga cggctcagga tacggataac
     1621 ggctactccg tgtttgagca gtcactgctg cggtatatcg ctgccgggct gggtgtctcg
     1681 tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg ggccagtgcg
     1741 aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg tcaggcgagc
     1801 cagatgtttc tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac gttaccttca
     1861 aaagcgcgct tcagttttca ggaagcccgc agtgcctggg ggaactgcga ctggataggc
     1921 tccggtcgta tggccatcga tggtctgaaa gaagttcagg aagcggtgat gctgatagaa
     1981 gccggactga gtacctacga gaaagagtgc gcaaaacgcg gtgacgacta tcaggaaatt
     2041 tttgtccagc aggtccgtga aacgatggag cgccgtgcag ccggtcttaa accgcccgcc
     2101 tgggcggctg cagcatttga atccgggctg cgacaatcaa cagaggagga gaagagtgac
     2161 agcagagctg cgtaatctcc cgcatattgc cagcatggcc tttaatgagc cgctgatgct
     2221 tgaacccgcc tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg ggatcagccg
     2281 cctgacggat gcggtgtccg gcgacagcct gactgcccag gaggcactcg cgacgctggc
     2341 attatccggt gatgatgacg gaccacgaca ggcccgcagt tatcaggtca tgaacggcat
     2401 cgccgtgctg ccggtgtccg gcacgctggt cagccggacg cgggcgctgc agccgtactc
     2461 ggggatgacc ggttacaacg gcattatcgc ccgtctgcaa caggctgcca gcgatccgat
     2521 ggtggacggc attctgctcg atatggacac gcccggcggg atggtggcgg gggcatttga
     2581 ctgcgctgac atcatcgccc gtgtgcgtga cataaaaccg gtatgggcgc ttgccaacga
     2641 catgaactgc agtgcaggtc agttgcttgc cagtgccgcc tcccggcgtc tggtcacgca
     2701 gaccgcccgg acaggctcca tcggcgtcat gatggctcac agtaattacg gtgctgcgct
     2761 ggagaaacag ggtgtggaaa tcacgctgat ttacagcggc agccataagg tggatggcaa
     2821 cccctacagc catcttccgg atgacgtccg ggagacactg cagtcccgga tggacgcaac
     2881 ccgccggatg tttgcgcaga aggtgtcggc atataccggc ctgtccgtgc aggctgtgct
     2941 ggataccgag gctgcagtgt acagcggtca ggaggccatt gatgccggac tggctgatga
     3001 acttgttaac agcaccgatg cgatcaccgt catgcgtgat gcactggatg cacgtaaatc
     3061 ccgtctctca ggagggcgaa tgaccaaaga gactcaatca acaactgttt cagccactgc
     3121 ttcgcaggct gacgttactg acgtggtgcc agcgacggag ggcgagaacg ccagcgcggc
     3181 gcagccggac gtgaacgcgc agatcaccgc agcggttgcg gcagaaaaca gccgcattat
     3241 ggggatcctc aactgtgagg aggctcacgg acgcgaagaa caggcacgcg tgctggcaga
     3301 aacccccggt atgaccgtga aaacggcccg ccgcattctg gccgcagcac cacagagtgc
     3361 acaggcgcgc agtgacactg cgctggatcg tctgatgcag ggggcaccgg caccgctggc
     3421 tgcaggtaac ccggcatctg atgccgttaa cgatttgctg aacacaccag tgtaagggat
     3481 gtttatgacg agcaaagaaa cctttaccca ttaccagccg c
//
LOCUS       DAZGCS010000239         3466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGCS010000239 DAZGCS010000000
VERSION     DAZGCS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.242"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3099)
                     /locus_tag="ACHK01_005035"
     CDS             complement(<1..3099)
                     /locus_tag="ACHK01_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO8099813.1"
                     /translation="MKRHLNTSYRLVWNHITGAFVVASELARARGKRGGVAVALSLAV
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKADGTIINQEGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETRINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFHARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQ"
BASE COUNT          665 a         1042 c          928 g          831 t
ORIGIN      
        1 ctgactgcta taaccttcag cgctgctgcc gttgatgctg tgggcataac cggcctgaac
       61 gcccagggtg atattttccc ggacacgggc ttccagtccg gcctgcagat ccagtgaagt
      121 gccattctgt gagggagaga acgtcatccc gctgcctgcg gtggaggtcc ccacacgcat
      181 atcaccccgg gagctgaagg tgcggataac ggaaggctgt acccaccagt tcaccggcag
      241 ttcacgcaca ctgtgttttg cactgtcacg caggggggca cgggatgagg tgccttcacc
      301 aaaggtcata tcgttgtggc tgcccagacg gaaaccggca cgcacatgct gtgcactgcc
      361 atgcccgaac ttcacataac cggcgttgtc cttaccgtca tccagcgaaa gcccctgcca
      421 ggtgtactgc agccgtggct ccagcatcag gttatcagtg atactgaagg gcagaccggt
      481 ttccagtgag cccagccagc cccggccccg tgcgtggaag tcgttattgc ccgatgacgc
      541 tttcatactg tggcgggttc cctgtgccac aatgtcagcc cacaggccgg aggaggtgtg
      601 tgtcaggttc atatatccgc ccaggctgcc ggcatcatcc cggaccgtgc cggcgcggga
      661 gccgtcatca tccttaacat caacggaaga atggcctgca gcaccatata cccctgcggt
      721 cacagacata ccggcaacct ctgttctcag caggtcaccc tccaggcgga cgaagccata
      781 gctgccgctg ctttccggcg tggccccacg ggcaataccg ccgttgttat cgtgaccgag
      841 atggccaccc tgaatgctga gacggacgct gttattttca ccgtttacac cggtctgatg
      901 gctgcgggag cctgccagaa tccggtcata gtccattgcc tgtgtcagca tggaggcgta
      961 cagggggact tcagcacgat aacgttcttc actgcgcaga taccagctct catcactgtc
     1021 ccggttgagg gagtagttaa aggccccggc ctgcagcata ttcccctgga caaaggcccc
     1081 ttcctccgtg gtggcaccgt taatggcctc aaccacctga atacccttac cggtggtcgc
     1141 cagccccgta ccactgttgc cggcgttcac caggttaagg atggtttttc cggttgccct
     1201 gccaccgtca atgaccagtc tgtcagcatt gttctgtgcc atatccgggc gtacacgcag
     1261 gctgatggtg ccattctgcc cgttcaggtt tttcacctgc agggttgtcg gaacgaactt
     1321 ccctgtgcgg gcggaggtga aatgaatctg tccggcatgg ctgaggtcat ccaccaccga
     1381 ctgaaccgtg gcgttatcgg ggatattcca ggtggcaccg gatgtcaggg tgacattcgt
     1441 ggggtcaatg gcaccgttca gcactgtgct gccggtcagc ttcagggccg tgccgcgctg
     1501 agcgatgaca tccgtggtga cggtactgtc gttcagcgtc agcgtgcctt cattcaggtt
     1561 gacggttttc tgggtgagtg tggtgttgct gacagtgagt gtgccacttc ctgatttttc
     1621 caccctgccg ttaccggtaa gagcgcctcc ctgcaggagt gcatcacctt cacggatggt
     1681 cagggtatcg ttattcaccg ttttcccgga aagggtaagt atggcgccgt tattcagcgt
     1741 ggtggtgccc gccagtgtgc cgcccctggc ggtgaacagt ccgccattta ccgtggtatc
     1801 cgtggccgta tcaccggcgt tcagcgtgaa tgaactgcct ttttccagca tcagggcatc
     1861 cgcctgaccg cctccgatac ggaatgcctt tccgtcgctc cgggtaccac tgacagcggc
     1921 accggaatcg gccagcagta caccgccatt ccccatggat acggtggtgg cggcaccacc
     1981 gttgcggaca tccagcgttc cgccatcatc cacacgggta ttagtggctg tgtgtccgct
     2041 cagcacatcc agacgtccgc cattttccag tacgacatta tcagccttac ccgccacaac
     2101 ggagaatgct cccaggcggt ttgtgccggt gacggttgca gctgtactgg taaccagtgc
     2161 accgcccgtg ttctgggtga catcagacgc tttaccgccg gcattcacct gcagctttcc
     2221 tttctggttg atggtggtat gcgcggcagt tcctccttcc ttaatcacct gccagccatc
     2281 acggtttatc cgcgtttctg ttgccgtgcc accgttgtgt acatactggt tacccccctc
     2341 cagccgggtg ttatgtgcct ctccgtgtac cgtctggtca ccaccggcgt aaatgagggt
     2401 gtcccgtgcc atccccgaag accagataac ctgccggcca tttttgttga tggtggtgga
     2461 ttcagccgtc cctccgacca tctgaccgct ggacacattt tcagactccg gaccaccttc
     2521 tgcaccggtg ttgacgatgg ttccggttgc cagtccgcca tgttttatgg tctggtaacc
     2581 ctcctggtta ataattgtac cgtcagcttt ccctcccccg tgtacccact gctcacctct
     2641 gttatccagc gtggtgttca ccgccagtcc gttaaggctc tgccctccgc catcacgaat
     2701 aaccgtatca ctggcagttc ctcctgcctg cacaatctga cgaccatttg cggtgacagt
     2761 ggtgtttctg cctgtgccac ccgcttttat ccattgcccg ccggtgtttt cgtcactgtc
     2821 cggccccagc tcaagcccgg tactgacagt cacgccatca gctgttccgg atacaaactg
     2881 gttgtcatgg tttaccagtg ttccgccatt cactgtttca cccgggtgca caacgatgtc
     2941 agcagccagc accgggagtg acgtgaccac ggcaagagac agtgcaaccg ccacaccgcc
     3001 acgtttaccc cgtgcgcggg ccagttcgga ggcaaccacg aaagcgcccg taatgtgatt
     3061 ccataccagc ctgtagctgg tgttcagatg tcgtttcatc agcttttcct tacagagggt
     3121 gaataaaaaa gccggtaccc acaaatgtgg ataccggcag gtacaaaaca gcatggtcag
     3181 ataaagtggt ttttcccgga cataaatgct tccactctct catccgccag agaagtatca
     3241 gtccattgtg tgcaattccg ttctgcatga tgaagagatt cagctgcctt tttgcaggcc
     3301 attaaccact ataaatgatc gatatcaacg atcgatacct gttttatcga tcgttttatt
     3361 ctattaacag caagacagtc atgcaagaga aagaggtggg tatgaggggg ttgtgtcatg
     3421 ttttcagttg gttaacgcaa tgtaatcagg catttcccct ggcaga
//
LOCUS       DAZGCS010000240         3406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGCS010000240 DAZGCS010000000
VERSION     DAZGCS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.243"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(636..833)
                     /locus_tag="ACHK01_005036"
     CDS             complement(636..833)
                     /locus_tag="ACHK01_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8099814.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(899..1169)
                     /locus_tag="ACHK01_005037"
                     /pseudo
     CDS             complement(899..1169)
                     /locus_tag="ACHK01_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1670)
                     /locus_tag="ACHK01_005038"
     CDS             complement(1398..1670)
                     /locus_tag="ACHK01_005038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099815.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            1990..2184
                     /locus_tag="ACHK01_005039"
     CDS             1990..2184
                     /locus_tag="ACHK01_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099816.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2527..2655
                     /locus_tag="ACHK01_005040"
     CDS             2527..2655
                     /locus_tag="ACHK01_005040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099817.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2965..3300)
                     /locus_tag="ACHK01_005041"
     CDS             complement(2965..3300)
                     /locus_tag="ACHK01_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099818.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1010 a          775 c          742 g          879 t
ORIGIN      
        1 ccaagtatat tatcttctgg tagagattca ggatacagtt tgtaggagct ttcttttttg
       61 aacaatgatt tgcaggacag ttatgctaaa ctagcggaat cagtatcaca acaataaatg
      121 actgatcata cctgaaattt gaattaacag gcatcatgaa tgtcaatagt ctaagcggtg
      181 actacttata tcgcttgcat aaatactaaa cactgactag aaaagtattg gataaccccg
      241 tcactaaaag taacgcttcg gttatttgct cggaatgtat taacgccaag attgttagcc
      301 gttacgcctt atatcactgg cgctgacaaa tgtttctttt ctaatttact gaaccggcgc
      361 agacacgcag gaaagcacgc cggcaccggt gtaatgccag ttctaatcaa taagaatgga
      421 atgacgaatc agtcaggaag ggataacaac ccgctgctga acggaaggta tcagcaggaa
      481 gatgaacggg aatatcgcca gcaccaaggg cagggccggg ttccacaata ccagcgctgg
      541 cattttgcac attgccagcg taccggcatt acaacacgtt tcatgctgct gccccccagg
      601 attcctcgat tcgttgctac atccactgct ctacttcact tccactgctc cacttcactt
      661 ttgtaccagc ggctgctgcg ccccagtttg atgggtttag ggaaatggcc ttcactgatc
      721 aacttgtaaa accatttgtc ggtcataccg gtgaaactgg tgatatagtt catgtcgatc
      781 agggaattat cggcacgcag aaatacgctg tcgtggcttg ttgtagtcaa catggtcaga
      841 atgtcctgtg agatttatga agattgtgcg aatgcgggaa atctactgag ctgtgctttc
      901 agaactggcc tgttacggga taccagggat taccggcggg gtaacgggct tccggatcat
      961 acacaccacg attatcgcgg acaaaatcac tgaacgccca tatcacctct ttaagtatgt
     1021 cttcgcagtc cggtaaatga cgatccagcg ccacatcccg ggtggtacag ctttgatgcg
     1081 cccggtgaca caaagcccgg attgttccag acatcctgaa tcaaacgccc cagattaggg
     1141 gcgtcgaaat atgcctctct gaccattata ttccggcgta taggtagcag gtctgaagtg
     1201 acaatgcgtc acctgacgtt aaaagtcacc caagatgacc ccagtggggg caacaagtat
     1261 aaaaccgttt tttgattaag tggtcggata tgaaaagggg aaggtttatg tgaaaaatac
     1321 ctgtgttttc tttgaaaaca cacaacagta tcaggttatt tatgtttata cagtgcgttc
     1381 caaactgacc tgaataatta cgcaagttgc aacaataaaa catttttgcc gtagtgtgag
     1441 gtactccggt aaattgcttc ccaggtgcag acaatgacta acgaagaact acaggcaatc
     1501 gcattatcca tgcttgagcg ccagcgcgaa aaagtacacc agcaacgaga gtctttgctt
     1561 aacccatctc cattcaccag gccaaaacga ttggagcttg aagcattctt ggacaaatac
     1621 ccacggcggc ttaaatcagg caaaaccaga ccaccaggct gatagctcac agtttgttct
     1681 ccgattcaat aaaacggata agaaccatca gcgcggtagc gtaaggcact acagcgcatt
     1741 gctttacgag taataacaat ggatttatca ccagaagacg caaagttttt acgagaactg
     1801 caacaccgta tgctcagaga agccattatc gaacagcaaa cgggcatgag cgccatagac
     1861 ccattgcgat acaaacgggc ctcatctcct ttagttgacg acaattccaa tgcggataaa
     1921 acacgtccac cacactgatt acatttttca ttagcacagt ggtgtgatgc agcatacgag
     1981 gaaaaccgga tgaatgagaa caaaataaaa aggctggagc aactgttaca ggcacgccaa
     2041 cgggagtttg ccactaaatg gggacgtgcc gcgctgccac atgaacgcct aacctatttt
     2101 ccacttgaag acctcattaa aaataatggc aaaaaaatca ggtctgataa gaaacacttc
     2161 ttagaatgca atgaagacaa ataacaacaa tctgcatatt gccaccaacc acagatgtgg
     2221 catcgttatc agcgcacttg attttcttta tatccactga cgagtttgcc ggtcattctg
     2281 gtatcatgca gatatttacg gcgtacaagc acactagcat gacattgtaa taaggcctga
     2341 gcctaacgct ttaatgtgtg tcatacactc agtggcgtaa tcgttgacaa tatggtgcat
     2401 gtaatcgtaa gcgtccagcg aaatccatct ggtcataatc tgaagcatcc gacaaagtgg
     2461 tgtccaccaa ataagtgatc tgcgcccaat atcctgagcc ggaactttga caaaggccaa
     2521 cgaaaaatgg gagcggatgt tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc
     2581 cagttctccg atctgtttaa ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg
     2641 atggagcatc gttaatacga tgctatacga tgcattcaca gtgcccggcc agaggatgcc
     2701 ccgtcgctgc atatggatca gggttggcaa tatcgaattg caggctatag gcaaagttaa
     2761 agccccatgg agtagcacaa aatatgccgc acaaaggaaa cggtctgaat aacgcagtga
     2821 tgaagaactt cttcagcaca ctgctaaaac gcatagtgat cgagcgctga ttctggcgaa
     2881 caactgaact aatacatcag aatctgcatt atgttaaata aatataaaaa gatggtttaa
     2941 ataccccgtt acttttgact tacactatac ggtatcgcat cgtttaatat tcgcaccggc
     3001 cagattttta tttctattag ttgtcacaat actgaatgcg tacgaccaca gtattctggc
     3061 tcctgtgtgg ttatgcttta attctgcgtt ccgggcagat aagcagttgc ttgcaggaat
     3121 ccttcttgtg ttaatgtcag ttcccctttt accagtgctg atttccacat tccgtccaac
     3181 agagcttata gcctttccct ggattatagc attgtccggc tgaagttctt tttgaataat
     3241 aatagaagca ctgctggcag atccagtccg tttttcataa cccactgtac tgataaccat
     3301 aatctaatca gtagaaattg agtcgaaaat aagcactact ccatacagga taattagagg
     3361 tcagtttgat tattcacaat tcatcatcag cattttctat ttctga
//
LOCUS       DAZGCS010000241         3403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGCS010000241 DAZGCS010000000
VERSION     DAZGCS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.244"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK01_005042"
     CDS             complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK01_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor YmfM"
                     /protein_id="HFO8099819.1"
                     /translation="MNSLTTHYRRSQLIALPVPGGKAKVEYCYAVNVPGGRVIVTHSF
                     AEWAVGDFNRQKETVLCDKLTAGSKITTE"
     gene            complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK01_005043"
     CDS             complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK01_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO8099820.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(761..1021)
                     /locus_tag="ACHK01_005044"
     CDS             complement(761..1021)
                     /locus_tag="ACHK01_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8099821.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            1119..1811
                     /locus_tag="ACHK01_005045"
     CDS             1119..1811
                     /locus_tag="ACHK01_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO8099822.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(2131..2646)
                     /locus_tag="ACHK01_005046"
     CDS             complement(2131..2646)
                     /locus_tag="ACHK01_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099823.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            2791..3102
                     /locus_tag="ACHK01_005047"
                     /pseudo
     CDS             2791..3102
                     /locus_tag="ACHK01_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3117..>3403
                     /gene="yfdP"
                     /locus_tag="ACHK01_005048"
     CDS             3117..>3403
                     /gene="yfdP"
                     /locus_tag="ACHK01_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO8099824.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLT"
BASE COUNT          931 a          817 c          758 g          897 t
ORIGIN      
        1 actccgtagt gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc
       61 ggttaaaatc acccacagcc cactctgcaa agctgtgggt tacaattacc ctgccacctg
      121 gtacattaac tgcatagcaa tactccacct tcgcttttcc acccggtaca ggaagcgcaa
      181 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacagtc
      241 acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt ctgaaatgca
      301 aaaaattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga ggtacagctc
      361 gtcgttaatt gctgtcttct catgcggttc cactacaccg tcttcaattg ctgaacgaat
      421 ctgttttgaa taactgccga tctgttcaat gacctccagc aggcgttggt tgatatcggc
      481 gttgtccaca tcctcgacat caggaagaga cacaaagacg ccatttgcag actgcgccac
      541 agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc atcccagcgg
      601 gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttctctg ttacatccag
      661 ccagtcagca gcttcagcgt acccccccgg caacgctgcg atagtttttc tgacagcttt
      721 cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg ttcacctcct
      781 gttcctgtgg tttaaaccca ttctggtttt ggctagattg aaaacgtgcc ggataaagaa
      841 tctgcatttc gctgacttca cccttaaaaa aattggctaa acgttctgca agctcgatag
      901 atggaatctg ctccagcctc tcaatacgac tcaacgtcgc tggattgact tgaacacccg
      961 cagcaacatg ctgcaaagtg aaaccatgcg ccttacgcac atttcgtaat ggtgattgca
     1021 tacatcctcc aaatattgcg cgttatgcat gttatttcac gcaattattt tgcgcaagtt
     1081 gatttgctta tcacgcaata aagaaatgta ataaacgcat gaacatagga aaccgagtca
     1141 gacaacttcg ccaagcgaag aacatgaaaa tcgccgatct cgctgaagcg ataggagtag
     1201 atgcggcgaa catctcgcgc ttagaaacgg gtaagcaaaa acaatttacc gaacaaacac
     1261 tgagtaatat tgccaagagc ttaggtgttg atattgctga tctctttacc tctgcccaca
     1321 aaagtaatac tgtatataaa aacagtaata atgaggatgt tgcgcaggtg aaggatgtgt
     1381 tccgtattga aatgctggat atcagtgcca gtgcgggaaa tggccttatc cagggcggtg
     1441 atgtcattga tgtgattcat gccatcgaat acagaactga taatgctgta tcaatgttcg
     1501 gcggacgacc agccaatcac atcaaagtta tcaacgttcg tggggacagt atgtgtccaa
     1561 ccattgagcc aggagatctc atcttcgttg atgtcagcat caatcagttt gatggtgatg
     1621 gtatatatgt ctttggtttt gatgacaaaa tatacgttaa aagacttcaa atgattcctg
     1681 acaaactgct ggtgatttct gataaccaga tttaccgtga atggggaatt actagcgaaa
     1741 acgaacaccg attcatggtc tttggaaagg tcttaatcag tcagtcgcaa acccttaaga
     1801 gacataatta acctcaatat cccatccatc ggccaccgaa aggtggcttt ttattaccta
     1861 taaatttgca tatcacgcaa atatcacttg catatctcgc aatttaattt tatcttttgt
     1921 tccagaccaa ctacaggatt acaacaaaat ctggttgcaa cacggtgcat gtgtcgtaag
     1981 cagtcagtaa atgtcaaaaa cgaacaggca ggacgcccac gaagtagccg cctggggcat
     2041 atgaagtcca ggatgattcg ttagcaacaa aaaagcgccc tacaggacgc ttagctcttt
     2101 aacaatctgg atatccacaa cagtagtaat ctacagattg ccgttaagtt ttctggacaa
     2161 ctcctcaatg gatggaggcg atacgtaatc cggattttta ttcatcagaa acttattttc
     2221 acagtggagg cacctgcttt tatgaaaaag ctcatcttcg ctaaccggga atggttgaag
     2281 tatcgatact atcttttgtc caaaacattt tgggcaaaga tgcatggtta tgctgccacc
     2341 gttcacgatt acctccttcg agtatacaaa agtacccgac tcaagttggt taaggatata
     2401 gccttccgtc tgagcctcaa agttttcgaa ttctgcaatt ttagctttga gagaagcatt
     2461 tatttcttga taagagccca ccagttcaac gagagacacg cattcgcgct gaatagacgc
     2521 aagctttgag ttcagctcac caatagccgc atttacttca gcttgagttt ttgcctcgtt
     2581 cattagtttt gcaatctggg ctgtttcacg aatagccgtc attgctgccg ttaattcagc
     2641 gatcacattg aatactctta ttgttgttgg ggatatccag attaaccgaa tccttgttgt
     2701 tggggaataa ccaggtccac ctcgcctgat gtggctaaaa gcaggcacat aacagctaag
     2761 tattttcaac cagagagaat ccttagcgtt gtggtgaatg cggctcagcg cacgcgggtt
     2821 aaggttgagg ctgacagtcg accttctgtg gatacccacc cgcctggtgt gcaaccttcg
     2881 ccaggcaccg ggaggcaccc ggcaccacaa ctttatgctg tgtgtagtcc tggcggtacc
     2941 agtttgtacc cttgcttccg gctggtaccg tccttttttg caaaacagag aagagcatca
     3001 ccggacgacg ggctcataac ccaatccatc cgggcggctg ccaccgcagg tgttcttctc
     3061 tgttttgtgg agaaaccaac cgaccttgca aggtcgatat gatgaggagc agcaaaatgg
     3121 ctagcgaacg cagtactgat gtgcaggcat ttatcgggga gctggacggc ggcgtatttg
     3181 aaaccaaaat cggcgcagtt ctcagtgagg tcgcttccgg tgtgatgaac acgaaaacca
     3241 aagggaaggt ctcactcaat ctggaaatcg aaccgtttga tgagaaccgt gtgaaaatca
     3301 aacacaaact ctcatatgtt cgcccaacta accgcgggaa aatttccgaa gaagacacca
     3361 ccgaaacgcc gatgtatgtc aatcgcggtg gtcgcctgac tat
//
LOCUS       DAZGCS010000242         3380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGCS010000242 DAZGCS010000000
VERSION     DAZGCS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.245"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            347..1663
                     /gene="shiA"
                     /locus_tag="ACHK01_005049"
     CDS             347..1663
                     /gene="shiA"
                     /locus_tag="ACHK01_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO8099825.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1765..3219
                     /gene="amn"
                     /locus_tag="ACHK01_005050"
     CDS             1765..3219
                     /gene="amn"
                     /locus_tag="ACHK01_005050"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO8099826.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          779 a          831 c          812 g          958 t
ORIGIN      
        1 gcaatacgtc tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata
       61 atgcaatgca gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg
      121 tttcatatct ctaacctgtg gctcctctgc ttaatcctta aacaacacca gcaactcctg
      181 cgctttcatc ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca
      241 gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
      301 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      361 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
      421 aggcagcttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc
      481 cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc
      541 cgcatttgct acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg
      601 tcactttggc gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      661 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      721 tattttactg gtgacgctgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
      781 cgcggcgttg ctttccgttg aaagtgcacc aaaaaataaa aaagcctttt acagtagcgg
      841 tgtacaagtt ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag
      901 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
      961 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     1021 tgaacaacag caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct
     1081 gttacgacat cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac
     1141 gatgtacatc gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
     1201 cgaacttttc ctcaatattg gtttgctggt aggtggatta agctgcttga caattccctg
     1261 ttttgcctgg cttgccgatc gttttggtcg ccgcagggtt tatatcacag gtgcgttaat
     1321 cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat
     1381 agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca
     1441 accgatgttt accgaaatgt ttggtgccag ttatcgctat agtggtgctg gagtcggtta
     1501 tcaggtcgcc agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac
     1561 ttactttgcc gggaactggc atagcgtcgc catttatttg ctggctggat gtctgatttc
     1621 cgcaatgacc gctttgttga tgaaagacaa tcaacgcgct tgatagtctg gcgaagatca
     1681 tccgatcttc gccttacact tttgtttcac attcctgtga catactatcg gatgtgcggt
     1741 aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag
     1801 gcactggata aactcgacgc gctgtatgag caatctgtag tcgcattacg caacgccatt
     1861 ggcaactata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt
     1921 gtctatccat cactgaccgt aacctgggac ggtagcacaa ccaatccccc caaaacgcgc
     1981 gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc
     2041 tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc
     2101 tcggtgcaac cctcgcagca tgaaatcccc tatccttatg tcatcgatgg ctctgaattg
     2161 acacttgatc gctcaatgag cgctgggtta actcgctact tcccgacaac agaactggcg
     2221 caaattggcg atgaaactgc agacggcatt tatcatccaa ctgaattctc cccgctatcg
     2281 cattttgatg cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta taccggtacg
     2341 ccagttgaac attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa
     2401 ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct
     2461 tgtgctggcg ggaactggat caccgccgaa accgaagcgc cagaagaagc catttccgac
     2521 cttgcatgga aaaaacatca gatgccagca tggcatttaa ttaccgccga tggtcagggt
     2581 attactctgg tgaatattgg cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg
     2641 gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagt
     2701 caggccattg gcgattatgt acttgcacac gcttatttac gcgatgacca cgttcttgat
     2761 gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat
     2821 gacgccacca agctggtgag cggcaggccc ggtgaggaag tcaaacagcg gctacgtact
     2881 ggtactgtgg taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt
     2941 cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa
     3001 ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat
     3061 ggcgagatta aacttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacat
     3121 ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattcg
     3181 cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttctgaa ccttcttatc
     3241 agaaatagat gtgaaggagt aagtaagaca cctggcaaat agcctgcaat aatcgtgggc
     3301 tgtttgcttc cttgggcgga tacgtgtttt attatcgtct taatgatttc cacatattaa
     3361 aagcaagtat gctttcaaaa
//
LOCUS       DAZGCS010000243         3370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGCS010000243 DAZGCS010000000
VERSION     DAZGCS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.246"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..173
                     /locus_tag="ACHK01_005051"
     CDS             <1..173
                     /locus_tag="ACHK01_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8099827.1"
                     /translation="RTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLTSE
                     KIAYTSIKSDEE"
     gene            complement(338..544)
                     /gene="ybcW"
                     /locus_tag="ACHK01_005052"
     CDS             complement(338..544)
                     /gene="ybcW"
                     /locus_tag="ACHK01_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO8099828.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            830..1240
                     /gene="ybcV"
                     /locus_tag="ACHK01_005053"
     CDS             830..1240
                     /gene="ybcV"
                     /locus_tag="ACHK01_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO8099829.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1531..1824
                     /gene="bor"
                     /locus_tag="ACHK01_005054"
     CDS             1531..1824
                     /gene="bor"
                     /locus_tag="ACHK01_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO8099830.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(1915..2097)
                     /gene="rzoD"
                     /locus_tag="ACHK01_005055"
     CDS             complement(1915..2097)
                     /gene="rzoD"
                     /locus_tag="ACHK01_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO8099831.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2314..2811)
                     /gene="rrrD"
                     /locus_tag="ACHK01_005056"
     CDS             complement(2314..2811)
                     /gene="rrrD"
                     /locus_tag="ACHK01_005056"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO8099832.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2811..3026)
                     /gene="essD"
                     /locus_tag="ACHK01_005057"
     CDS             complement(2811..3026)
                     /gene="essD"
                     /locus_tag="ACHK01_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO8099833.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          997 a          766 c          666 g          941 t
ORIGIN      
        1 gcagaacaac aacccgcaac atccgctttc ctaaccaaat gattgaacaa attaacatcg
       61 ctcttgatca aaaagggtcc gggaatttct cagcctgggt cattgaagcc tgccgtcgga
      121 ggctaacgtc agaaaagata gcatatacat caattaaaag tgatgaagaa tgaacatccc
      181 gcgttcttcc ctccgaacag gacgatattg taaattcact taattacgag ggcattgcag
      241 taattgagtt gcagttttac cactttcctg acagtgacag actgcgtgtt ggctctgtca
      301 caggttaagt agtttgaatg attagcagtt atggtgatca gtcaaccacc agggaataat
      361 ccttcatatt attatcgtgc ttcaccaacg ctgcctcaat tgccctgaat gcttccagag
      421 acaccttatg ttctatacat gcaattacaa catcagggta actcatagaa atggtgctat
      481 taagcatatt ttttacacga atcagatcca cggagggatc atcagcagat tgttctttat
      541 tcattttgtc gctccatgcg cttgctcttc atctagcggt taaaatatta cttcaaatct
      601 ttctgtatga agatttgagc acgttggcct tacatacatc tgtcggttgt atttccctcc
      661 agaatgccag caggaccgca ctttgttaca caaccaatac tattaattga aaacattcct
      721 aatatttgac ataaatcatc aacaaaacac aaagaggtca gaccagattg aaacgataaa
      781 aacgataatg caaactacgc gccctcgtat cacatggaag gttttaccaa tggctcaggt
      841 tgccattttt aaagaaatat tcgatcaagt gcgaaaagat ttaaactgtg aattgtttta
      901 ttctgaacta aaacgtcaca atgtctcaca ttatatttac tatctagcca cagataatat
      961 tcacattgtg ttagaaaacg ataacactgt gttaataaaa ggacttaaaa aggttgtaaa
     1021 tgttaaattc tcaagaaata cgcatcttat agaaacgtcc tttgataggt tgaaatcaag
     1081 agaaatcaca tttcagcaat acagggaaaa tcttgctaaa gcaggagttt tccgatgggt
     1141 tacaaatatc cacgaacata aaagatatta ctataccttt gataattcat tactatttac
     1201 tgagagcatt cagaacacta cacaaatctt tccacgctaa atcataacgt ccggtttctt
     1261 ccgtgtcagc accggggcgt tggcataatg caatacgtgt acgcgctaaa ccctgtgtgc
     1321 atcgttttta attattcccg gacactcccg cagagaagtt ccccgtcagg gctgtggaca
     1381 tagttaatcc gggaatacaa tgacgattca tcgcacctga catacattaa taaatattaa
     1441 caatatgaaa tttcaactca ttgtttaggg tttgtttaat tttctacaca tacgattctg
     1501 cgaacttcaa aaagcatcgg gaataacacc atgaaaaaaa tgctactcgc tactgcgctg
     1561 gccctgctta ttacaggatg tgctcaacag acgtttactg ttcaaaacaa acagacagca
     1621 gtagcaccaa aggaaaccat cacccatcat ttcttcgttt ctggaattgg gcagaagaaa
     1681 actgtcgatg cagccaaaat ttgtggcggc gcagaaaatg ttgttaaaac agaaacccag
     1741 caaacattcg taaatggatt gctcggtttt attactttag gcatttatac tccgctggaa
     1801 gcgcgtgtgt attgctcaaa ataattgcat gagttgccca tcgatatggt cagctctatc
     1861 tgcactgctc attaatatac ttctgggttc cttccagttg tttttgcata gtgatcagcc
     1921 tctctctgag ggtgaaataa tcccgttcag cggtgtctgc cagtcggggg gaggctgcat
     1981 tatccacgcc ggaggccgtg gtggcttcac gcactgactg acagactgct ttgatgtgca
     2041 accgacgacg accagcggca acatcatcac gcagagcatc attttcagct ttcgcatcag
     2101 ctaactcctt cgtgtatttt gcatcgagcg cagcaacatc acgctgacgc atctgcatgt
     2161 cagtaattgc cgcgttcgct agcttcagtt ctctggcatt tttgtcgcgc tgggctttgt
     2221 aggcgattgc gttatcacgg taatgattga ccgcccatga caggctgacg atgatgcaga
     2281 taatcagagc ggatataatc gcggttactc tgctcactgt tgcccccaca aacagacttc
     2341 acgctcaatc tcacgacgag tcatcaggcc tttccattgc ttaccgccag cgtatgtcca
     2401 gcgacgcagc tgatcacatg cgcctttgat atcgccctgg tttattttgc gaagaagcgt
     2461 cgatgttcta aaattgccag cacccacatt gtaaacgaat gagtaaagag cgccgcgcgt
     2521 tgtttccggt atatcaactt tgatatacgg gttaatttgt ctggcgacag tggcaaggtc
     2581 tttattcaag agtgctttgc attctgcttt ggtatacgtt ttaccgagca tgatgtcttt
     2641 tccggtgtgt ccgtgacata cagtccatac accaacaata tctttgtatg gtatgtagct
     2701 gacaccttcc agaccatcgt taccacttgg gccagtgatt aacactgatg ctatagcaat
     2761 tgctccgcca ccaatagcag cagcaacggc ttttcgtaat gatggaggca ttattcacct
     2821 ctcgcagcct tgcgcttatc ttctttaatc ttgaaataaa ggtttgtcag gtacgtcagc
     2881 aggccaaata ccaggctacc cagcacacct attgctgccc actgtgaggg agtgacttta
     2941 tcgagcagct gtaaaaacca gtaaccggca ctacctgctg aggtgccata ggcgactcca
     3001 gttgttaact tatccatgaa tttcataacc ccacctcgca gacaaagcgg gtgtaaattg
     3061 agggaataca acgtatcgca aaaaagcaga aacgtaactg actccgagtc agtgaataac
     3121 tcaggtattg agttatcagc taatatcgag actcaaaaaa tggaaaaacc agctcgacgg
     3181 cgggtttaag ctgtgtgacg aagtaaccac tcttaacagc ataaccaatt ttttacgtac
     3241 gtaaaccact gaatgatatt tatgagaatg ctaccgagtg ttcaaaacat caccacaaat
     3301 acataagaaa acctcaacaa ataaccaata aataatttcc agtgttattt ttagccggtt
     3361 taaattaaac
//
LOCUS       DAZGCS010000244         3358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGCS010000244 DAZGCS010000000
VERSION     DAZGCS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.247"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..191
                     /locus_tag="ACHK01_005058"
     CDS             <1..191
                     /locus_tag="ACHK01_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO8099834.1"
                     /translation="TEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQR
                     DNALLMIGKVIDYKEDVI"
     gene            249..827
                     /gene="espA"
                     /locus_tag="ACHK01_005059"
     CDS             249..827
                     /gene="espA"
                     /locus_tag="ACHK01_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO8099835.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNEVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIAEVQSSSNKDAKAK
                     LPQEVIDYIKDPRNGITVSDVKDITVELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARADIQSLQYRTISAISLGK"
     gene            840..1982
                     /locus_tag="ACHK01_005060"
     CDS             840..1982
                     /locus_tag="ACHK01_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO8099836.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSAAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            2003..2947
                     /gene="espB"
                     /locus_tag="ACHK01_005061"
     CDS             2003..2947
                     /gene="espB"
                     /locus_tag="ACHK01_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO8099837.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQQTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            2954..>3358
                     /locus_tag="ACHK01_005062"
     CDS             2954..>3358
                     /locus_tag="ACHK01_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099838.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
BASE COUNT          978 a          667 c          807 g          906 t
ORIGIN      
        1 taacagaaga ctggctcctg gaaagccctt ctcgggtatc gattgtcgaa gataaacatg
       61 tttattattt ccacctattg aaagactttt ttgcatcatt acctgacgct tgttttattg
      121 atagggaaca gagagataat gcattattaa tgattggtaa agtaattgat tataaggagg
      181 atgttatttg atattgattt tttaattgtt tttggtcttg ctaagaaaga ttattaagag
      241 gtatatacat ggatacatca actgcaacat cagttgccag tgcgaacgcg agtacttcga
      301 catcgacagt ctatgactta ggcagcatgt cgaaagacga agtagttcag ctatttaatg
      361 aagtcggtgt ttttcaggct gcgcttctca tgtttgccta tatgtatcag gcacaaagcg
      421 atctgtcgat tgcaaagttt gctgatatga atgaggcatc taaggagtca accactgccc
      481 aaaaaatggc taatcttgtg gatgctaaaa ttgctgaggt tcagagcagt tctaacaagg
      541 atgcgaaagc caaacttcct caagaagtga tcgactatat aaaagatcct cgtaatggca
      601 ttacggtcag tgatgtaaaa gacattactg ttgaattagg cgctggtgat ttgcaaacgg
      661 tgaaggcagc tatttcggcc aaatcgaata acttgaccac ggtagtgaat aatagccagc
      721 ttgaaataca gcaaatgtca aatacgttaa acttattaac gagtgcacgt gctgatattc
      781 agtcactgca atacagaact atttcagcaa tatcccttgg taaataaccg gagataacta
      841 tgctcaatgt aaatagcgat atccagtcga tgaggtctgg ggccagcgct gctacggcta
      901 cttcaggtat taatcagcct gaagtgactt ccgcgctgga tttacaactg gttaaatcca
      961 cggccccatc agcaagttgg acagaaagta ccgctttacc aacgccaccg gcaggccact
     1021 cattagtgac gccctctgct gctgaggatg tcctgagtaa actgtttggt ggtattagtg
     1081 gtgaagttag gagtcgaaca gagggaaccg aaccacagcg cagcactcag aatgcaagct
     1141 ctggctatcc ttatctttct caggtgaata acgttgaccc tcaggccatg atgatgatgg
     1201 tcaccctgtt atccctggat gcttcagcgc agaaagttgc gagtatgaaa aactccaacg
     1261 agatctacac ggatgggcaa aataaggcgc tggataataa aacgctggag tttaaaaagc
     1321 agctcgaaga acaacagaaa gccgaagaga aagcacaaaa aagtaaaatt gttggtcagg
     1381 tctttggttg gttgggcgtc gcggtaactg ctattgcagc tatttttaac ccggcactct
     1441 gggctgttgt tgccgttagc gcaacagcaa tggcactgca aactgcagtt gatgtaatgg
     1501 gcgataaggc cccgcaggcg ttaaagacag cagcgcaagt ctttggcgga gtatctattg
     1561 ctgcaagtat tctgacagcc ggcattggcg gggtgtcttc acttatgtct aaagttggcg
     1621 atgtcgctaa caaagttggt tcaaacatcg tgaaaacagt tactaaactg gccgatgtat
     1681 ttgttgagaa cgttgtttca aaagttgcgg caacggcaaa tggatttacg acctctgcac
     1741 gttctattgg gacaactgtg ctgaataatg attctgcgca atacagcata ttggctcagt
     1801 tatctgctta cgcggtgcaa aacttaactc gacagagtga aaacctgggt gagagtgcga
     1861 aggttgagct ggataaagct gcagccgagt tgcgaaacca ggcaagctat ttacaaaatg
     1921 cttctcagtt aatatccgac tcagcacgag taaatagccg tattgttagt ggtcgagttt
     1981 aattattaaa gagaatttaa ttatgaatac tattgattat actaatcaag taatgacggt
     2041 taattctgtt tcggagaata ctaccggctc taatgcaatt accgcatctg ctattaattc
     2101 atctttgctt accgatggta aggtcgatgt ttctaaactg atgctggaaa ttcaaaaact
     2161 cctgggcaag atggtgcgta tattgcagga ttaccaacag caacagttgt cgcagagcta
     2221 tcagatccaa ctggccgttt ttgagagcca gaataaagcc attgatgaaa aaaaggccgc
     2281 tgcaacagcc gctctggttg gtggggctat ttcatcagta ttggggatct taggctcttt
     2341 tgcagcaatt aacagtgcta cgaaaggcgc gagtgatatt gctcaacaaa ccgcctctac
     2401 atcttctaag gctattgatg cggcttctga tactgcgact aaaacgttga ctaaggcaac
     2461 ggaaagcgtt gctgatgctg ttgaagatgc atccagcgtg atgcagcaag cgatgactac
     2521 agcaacgaga gcggccagcc gtacatctga cgttgcagat gacattgccg attctgctca
     2581 gagagcttct cagctggctg aaaacgctgc agatgccgct cagaaggcaa gtcgggcaag
     2641 ccgctttatg gctgcagtag ataagattac tggctctaca ccatttattg ccgttaccag
     2701 tcttgccgaa ggcacgaaga cattgccaac aacggtatct gaatcagtca aatctaacca
     2761 tgagattagc gaacagcgtt ataagtctgt ggagaacttc cagcagggta atttggatct
     2821 gtataagcaa gaagttcgca gagcgcagga tgatatcgct agccgtctgc gtgatatgac
     2881 aacagccgct cgcgatctca ctgatcttca gaatcgtatg ggtcaatcgg ttcgcttagc
     2941 tgggtaattg atcatggtcg atacgtttaa tgatgaagtg cttaatcact atcttgaaca
     3001 aaaagggtac acaatacaga aggagtttct ttgtggcagt gcctttttta tcggatggcg
     3061 gattgagacc tcttttttct cattagcgta cagacttgat gaacaagaac tgattttgtg
     3121 ctcttttgaa gcacgtaacc aaacagggct taacggccct gttttatcac tgactcgctt
     3181 gctcgaagag ttgtaccacc atttttcggg tattaagaaa atcagtgcga tgaaatctaa
     3241 gattggttca gattcagaac gtcaaaagcg cgaagagttg tttaattact tcatcagaaa
     3301 gggcgctgtg cagcaagaaa cagaagacgg aatttggttc gtaatgaatg taaatagt
//
LOCUS       DAZGCS010000245         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGCS010000245 DAZGCS010000000
VERSION     DAZGCS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.248"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK01_005063"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK01_005063"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(210..737)
                     /gene="mobB"
                     /locus_tag="ACHK01_005064"
     CDS             complement(210..737)
                     /gene="mobB"
                     /locus_tag="ACHK01_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO8099839.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(719..1303)
                     /gene="mobA"
                     /locus_tag="ACHK01_005065"
     CDS             complement(719..1303)
                     /gene="mobA"
                     /locus_tag="ACHK01_005065"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO8099840.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1373..1642
                     /locus_tag="ACHK01_005066"
     CDS             1373..1642
                     /locus_tag="ACHK01_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO8099841.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1719..2705
                     /gene="srkA"
                     /locus_tag="ACHK01_005067"
     CDS             1719..2705
                     /gene="srkA"
                     /locus_tag="ACHK01_005067"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO8099842.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEDYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2722..3348
                     /gene="dsbA"
                     /locus_tag="ACHK01_005068"
     CDS             2722..3348
                     /gene="dsbA"
                     /locus_tag="ACHK01_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO8099843.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          843 a          808 c          831 g          868 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcaga
      121 aggcccagtc ggaagactgg gcctttttgc gctggtgcag aaaaaggtcg gatgcgacac
      181 tggctgcgtc tgatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      241 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      301 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      361 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      421 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      481 tcttcgtctg gtgtttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      541 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      601 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      661 tgtttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      721 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaata aatgcatctt
      781 tattatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc attacccggc
      841 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      901 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      961 gattaagccg ggctgctaaa tcctcgggaa tataaggagt atcgcacgga caaaacaaaa
     1021 accactcgcc tgcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
     1081 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatggcgat
     1141 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1201 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1261 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1321 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1381 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1441 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1501 ggatttgacg gatgacattc tgatctatca tctgaaaatg cgtgattccg cgaaagatgc
     1561 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1621 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1681 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1741 tccagacact acacccggat accatcatgg atgctctatt tgagcaaggg atccgggtgg
     1801 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1861 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1921 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1981 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     2041 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     2101 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2161 gtctgaatga atatctcatt gagcctcgca agctgtttga ggacgctaca ctgatacctt
     2221 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2281 actggcggga agacttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2341 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2401 aggatttgtg gatgttgctc aatggcgata aagccgagca gtggatgcaa ctggaaacta
     2461 ttattgaaga ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2521 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2581 ccgcgttccc gaaaaatttt ccgtggttaa ccggggaaga ttactggctg cgacagacgg
     2641 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2701 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2761 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2821 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2881 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2941 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     3001 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tgactgttcc gctgtttgaa
     3061 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3121 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3181 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3241 gttaacggta aatatcagct gaatccgcag ggcatggata ccagcaatat ggatgttttt
     3301 gttcagcagt atgctgatac agtgaaatat ctgtccgaga aaaaataatt
//
LOCUS       DAZGCS010000246         3343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGCS010000246 DAZGCS010000000
VERSION     DAZGCS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.249"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..285)
                     /locus_tag="ACHK01_005069"
     CDS             complement(25..285)
                     /locus_tag="ACHK01_005069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099844.1"
                     /translation="MLSKLTSVQFECVSLIEMVRSYQEEAASLKAKIAEIEDFQQQAE
                     GYKLNKLDSGTLVYSKKQFIGDTEITVHLCPQCFGKKVISVL"
     gene            938..1126
                     /gene="dicB"
                     /locus_tag="ACHK01_005070"
     CDS             938..1126
                     /gene="dicB"
                     /locus_tag="ACHK01_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8099845.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            1123..1311
                     /locus_tag="ACHK01_005071"
     CDS             1123..1311
                     /locus_tag="ACHK01_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8099846.1"
                     /translation="MTSAFALIMTVFLITGEPQNVITGIYYSKSSCIQVRDEQKIPGE
                     CLPLKKVSLYLNNETPAG"
     gene            1404..>3343
                     /locus_tag="ACHK01_005072"
     CDS             1404..>3343
                     /locus_tag="ACHK01_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO8099847.1"
                     /translation="MNADKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VAAMDDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMILIAPS
                     VPDKPASTDNTNVNGEDMAKIEENMLLPVSGQILPVRWLAQHNSEKPLTHVSLDKLRA
                     LHNAQDEKLPAVTALAISNKAVQLEPLELRDLYKLVRDTDKVFPAPVNSDLGLMTSFI
                     EAYLGADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGILTDRGEGFVHDDASVARD
                     VATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFFSKFITMPGHKDYSRAIVV
                     ASVKEAPIGIEATPHRVTEYLNKVLTETDHATPDPEIVDIACGRSSAPMPQRVTKEGK
                     QDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELH
                     EARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQND
                     QKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQET
                     FDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRIITS
                     TSRTCDHLMIDLETMGKNPDAP"
BASE COUNT          958 a          797 c          798 g          790 t
ORIGIN      
        1 actctccagg aaacccgggg cggttcacag tacagatatt accttttttc caaaacattg
       61 tgggcaaaga tgcactgtta tctcggtatc gcctataaat tgcttcttgg aatacacaag
      121 agttccagaa tcaagcttgt taagtttgta accttccgcc tgttgctgaa agtcttctat
      181 ctctgcaatt tttgctttga gagatgcggc ttcttcctga taagagcgca ccatctcaat
      241 gagcgatacg cattcaaact gaacagacgt aagttttgaa agcatatcgc tgacagcgga
      301 atttatctca gtttgagttt tgcattcaga ataccctttg ccattaccgc agtttctttg
      361 attgccgaca ttgctgctga gaactcaact atcacgttac atactcttct cgttgttggg
      421 gatatccaga ttatacaaat ttcttgttgt tggggaataa caggaaccac ctcgcctgac
      481 gtggttaaaa gcaggcacac aacgcgaaag cgtacggcga ggtagctggt tcatagatag
      541 cctgtcgtta aattttcgtc gaccgtgcgc ttccggttgt ggcagtccgc gaaatggcgc
      601 ggcggtaagt atggctgggg tttcctccat tgctccagaa aatgcaccgg gttgtcaggt
      661 tgaccatacg cttaagtgac aaccccgcta caacgccctc tgttatcaat tttctggtga
      721 catttggcgg tatcagtttt actccgtgac tgctctgccg ccctttttaa agtgaatttt
      781 gtgatgcggt gaatgcggct aagcgcacgc ggaacagtta aaaccaaaaa cagtgttatg
      841 ggtggattct ctgtatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag
      901 gcactgcatc acaaaattca ttgttgagga cgcgataatg gaaacgttat taccaaacgt
      961 taatacgtct gaaggttgtt ttgatattgg agttctgctc agtaaccggg agtttactga
     1021 agatgccatt aagatgagaa aatatgaacc ttatcttctt aatgataatt ccatactttc
     1081 cagaattgcc cttcttgaac ttggtattat cggagaacag cagtgacttc agcgtttgca
     1141 ctgataatga ccgtttttct tataacgggt gagccgcaga atgtgattac cggaatttat
     1201 tacagtaagt catcctgcat tcaggtaagg gacgaacaaa aaatccccgg tgaatgcctc
     1261 ccgttaaaaa aagtatcgct gtacctgaat aacgaaacac cggctggata acccgccagc
     1321 catattaacg ccataccaac ggattaaaaa tgccagcaat ggcaggaatt tgttcatcct
     1381 taaaatggtt atgaggttta tcaatgaacg ctgataaaga agaaattgca ctatattacg
     1441 aagccaaaaa tgacaaagtc agaaaacgcc ttgggattaa aggtggtttt tactggcgca
     1501 cagcaaaaaa attatcggtt gcaatatcac gtggtgttgc tgcaatggac gatgccggat
     1561 ttgacgaaga ggattttaaa aaacccatcc gcgtccattt acccgttgtg aatgaccttc
     1621 caccggaagg tgtgtttgat actgaattct gcaaccgata cgaaaaaggc ggagaagatg
     1681 gtatcacaat gatacttatc gcgccctctg ttccggacaa accagccagt actgacaata
     1741 ccaacgtcaa cggcgaagac atggctaaga ttgaggagaa tatgcttctt ccggtttcag
     1801 gtcagattct gcctgttcgc tggctggcgc aacacaacag cgaaaaaccg ctcacgcacg
     1861 tttcactgga caaactgcgc gcattacata acgcacagga tgaaaaactt cccgccgtta
     1921 ccgcgctggc catctcaaat aaagcagtgc aactcgaacc gctggagctt cgggatttgt
     1981 acaaactggt gcgagacact gacaaagttt tccccgcccc cgtaaattcg gacctgggac
     2041 tgatgacctc ttttatcgaa gcatacctgg gcgctgacta caccgatcgc ggtctgctta
     2101 caaaagagtg gatgaaagga aatcgtgttt cacgcatcag ccgcacgcca tccggcgcta
     2161 acgctggcgg cggcattctt accgatcgcg gcgaaggttt tgtccacgat gatgcgtcag
     2221 tggcgcgtga cgtagccact ggcgtactgg cccgttcaat ggacgtggat atttataacc
     2281 ttcatcccgc gcacgctaaa cgcattgagg aaattatcgc tgaaaataaa ccgccatttt
     2341 ctgtgttttt cagcaaattc atcaccatgc cgggccacaa ggattactcc cgcgccatcg
     2401 tggttgcgtc cgtgaaagaa gcaccaattg gtatagaggc tactccccac cgtgtcaccg
     2461 aatacctgaa taaggtgctg actgaaaccg atcatgccac ccctgatccg gaaatcgtgg
     2521 atattgcctg tggtcgctcc tctgccccga tgccgcagcg tgtaacaaaa gaaggaaaac
     2581 aggatgatga agaaaaaccg cagccatctg gcgcaatggc agatgaacag gcaacgactg
     2641 aagcagtgga accggataca actgaacata atcaggacac gcagtcgatg gatgctcagc
     2701 cacagataaa ttctgttgat gcgaaatatc agaaactgcg ggcagaactc catgaagcca
     2761 ggaaaaacat tccgccccaa aatcctgtcg atgcagacaa attactggct gcctctcgcg
     2821 gagaatttgt tgaagggatt agcgacccga atgatccgaa atggattaag gggatccaga
     2881 cccgcgattc tgtgtaccag aatcagccag aaacggaaca gaacgaccag aaagcggaac
     2941 agaacagccc aaatacgcaa caaaacgagc cagaaacgaa acaacctgaa ccagtagtgc
     3001 aacaggaacc ggaaaagatc tgcaccgcct gcggtcagag gagtggcggc aactgccctg
     3061 attgtggcgc ggtgatgggc gacgcaacat accaggaaac attcgatgac aagaacctgg
     3121 ttgaagttca ggaagacgat tcggagaaaa tggaaggcgc tgaacatcca cacaaggaga
     3181 atgctggcag cgctcaggac cacgccagcg atagtgaaac tggcgagacg gcagatccct
     3241 taattacggt gaacggtcat cgcattatca catccaccag caggacgtgt gaccatctaa
     3301 tgatcgacct tgaaaccatg ggaaaaaatc ctgatgcccc gat
//
LOCUS       DAZGCS010000247         3293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGCS010000247 DAZGCS010000000
VERSION     DAZGCS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.250"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..174
                     /locus_tag="ACHK01_005073"
     CDS             <1..174
                     /locus_tag="ACHK01_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099848.1"
                     /translation="YCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHVSAFT
                     GIYNDLAAASACM"
     gene            171..752
                     /locus_tag="ACHK01_005074"
     CDS             171..752
                     /locus_tag="ACHK01_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8099849.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTIIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(943..1470)
                     /gene="sodC"
                     /locus_tag="ACHK01_005075"
     CDS             complement(943..1470)
                     /gene="sodC"
                     /locus_tag="ACHK01_005075"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO8099850.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1604..>3293
                     /locus_tag="ACHK01_005076"
     CDS             1604..>3293
                     /locus_tag="ACHK01_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8099851.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLMDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPERPFNIRMVRETADSTPDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLVAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWSGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSL"
BASE COUNT          751 a          866 c         1012 g          664 t
ORIGIN      
        1 tactgcggca acggtgagct gcttcaccat ctgcctgaac aactgagtaa acgggagagg
       61 tattccgaaa aatggcaacg acgaacgcat tcagcctggc gtcaccgcca ctggcacgta
      121 tctgccttca cggggattta caacgatttg gccgccgcct cagcctgtat gtgaacacgg
      181 cagcggaagc catccgtgcc ctgtcgatgc agatgccggg attccgccgt cagatgaacg
      241 aaggctggta ccagatacgt attcgcggtg aggacacggc accggaggcg gtgtacgccc
      301 gtcttcacga acaactgggt gagggaacga tcatccacat tgtgccgcga ctggccgggg
      361 ccggaaaggg tggactgcag attgtgctgg gggcggcagc catcgtgggc tctttcttca
      421 ccgccggcgc aacgatggcg ttatggggtt cagccctggc agccggtggt ttttctgcca
      481 ccacgatgct gttttcactt ggagccagca tgatactggg tggtgtggct cagatgcttg
      541 ccccgaaggc aaaaacaccg gattaccgcg caacggataa cggcagacag aacacgtatt
      601 tttcctcact ggacaacatg attgctcagg ggaacccgat gccggtgcct tacggtgaaa
      661 tgctggttgg ctcacggcga atctcccagg acatcagtac ccgtgatgaa ggcggtgacg
      721 ggaaggtggt ggttatcggg cggcaggcat aaaagcgaaa aaatcccgca gtgctcacgg
      781 acaggaactg cgggagcgtt acgaagattg agtgtaagga attattctta tgtcacgaca
      841 aaaacattaa ctcagagagg gaggatgtgc cgttcttttc agggagaaag gatttatcgt
      901 cctgaggaat aaaggtaagg ggcccgcccc ttacctgact gattattgaa tgatgccgca
      961 ggccattctc gcaccaccac cgcccagggg ctccggatgg tcatgatggt tatcaccgcc
     1021 agcatgaagc atgagagaac gccctttaat ctcttttaat gagttcagtc tcggggccag
     1081 gaccgggtag ttcgcttttc cgtcatgcgt cacgaacagc gcagggaggt cgcccaggtg
     1141 tccatccgga gaccaggggc caagatgttt gccggtgttt ttcgggtcaa agtgaccgcc
     1201 agccgataat gctgcgaccg gttttccgtc tttcagtgcc ggggcgcaat ttcctttttc
     1261 gtgcacatga aaaccatgaa tgccttcaga cagagagtga agggctggtg tgaacagcag
     1321 accgtagggg gtctcctgaa tggttatttt tccaatgctg acttcttttc cgtcagcact
     1381 gacaaggttc attgggactt cctgttctgc tgcgtatccg catgatgctg ctgtcagcat
     1441 ggcaatggca gcaatgattt tacatttcat aaaaccctca ttaattccgt taacagactg
     1501 agcttgctgg ttacagggta acaaacagcg ttctgatgat atcgcgcaat agctgtgcaa
     1561 tatcctatca ctgcgattaa taataccaat tgagaggaac attatgggca aaggtggcgg
     1621 cagggcgcac acgcctcgag aggcgaagga taatctcaaa tccacgcaga tgatgagtgt
     1681 gattgatgcg attggtgagg gaccgataga aggcccggtg aaggggctgc agagtatcct
     1741 ggtgaacaaa accccgctga tggacacgga cggtaatccc gtgatacacg gtgtgactgc
     1801 ggtctggcgt gccggggagc aggagcagac accaccggaa ggctttgagt cctccggagc
     1861 tgaaaccgga ctgggcgtgg aagtgacgaa ggcaaagccg gtgacgcgca ccattacgtc
     1921 cgcgaacatt gaccgcctgc gggttacctt cggggtgcag tcactggtgg agaccacctc
     1981 aaagggtgac cgtaacccga cgtctgtccg cctgctgatt cagttgcagc gtaacggtaa
     2041 ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc acctcacagt acctggcgtc
     2101 ggtgattctg gagaatctgc ctgagcggcc ctttaacatc cggatggtcc gggagacagc
     2161 ggacagcacc ccggaccagc tgcagaataa gacgctctgg tcgtcataca ccgaaatcat
     2221 cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg ctgcaggtgg atgcggagca
     2281 gtttggcggt cagcagatga cggtgaacta ccatatccgc ggtcgcatca tccaggtacc
     2341 gtcaaactat gacccggaaa aacgcacgta cagcggcatc tgggacggca gcctgaaacc
     2401 ggcatacagc aacaacccgg cctggtgcct gtgggacatg ctgactcacc cgcgctatgg
     2461 catgggaaaa cgtctggtgg cggcggatgt tgacaagtgg gcgctgtatg ccatcgggca
     2521 gtactgcgac cagacggtcc cggatggttt cgggggcaca gagccgcgga tgacctttaa
     2581 tgcgtacctg tcacaacagc gtaaggcgtg ggacgttctc agtgatttct gctcggcgat
     2641 gcgctgtatg ccggtatgga gcggccagac gctgacgttc gttcaggacc gcccgtcgga
     2701 tgtggtgtgg ccgtacacca acagcgatgt ggtggtggat gataacggcg tgggattccg
     2761 ctacagcttc agtgccctga aggaccggca cacggcggtg gaggtgaatt acaccgaccc
     2821 gcagaacggc tggcagacct ccactgaact ggtggaagac ccggaagcca tactgcgcta
     2881 cggacgcaac ctgctgaaga tggacgcgtt cggctgtacc agccgcggtc aggcccaccg
     2941 tgccggactg tgggtgataa agaccggact gctggaaacg cagacggtgg atttcacgct
     3001 cgggtctcag gggctgcggc acacacccgg tgacatcatt gaaatctgtg ataacgacta
     3061 tgccgggacc ctgaccggcg gacgtgtcct gtccattgat gccgccagcc gcaccctgac
     3121 actggaccgt gaggtgaccc tgccggagac aggtgccgcc acggtgaacc tgattaacgg
     3181 cagcggtaag ccggtgagtg tggacatcac cgcacacccc gcgccggacc ggatacaggt
     3241 cagcaccctg cctgatggtg tggagacata cggtgtatgg ggactctccc tgc
//
LOCUS       DAZGCS010000248         3289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGCS010000248 DAZGCS010000000
VERSION     DAZGCS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.251"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..366
                     /locus_tag="ACHK01_005077"
     CDS             <1..366
                     /locus_tag="ACHK01_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO8099852.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
     gene            363..1043
                     /locus_tag="ACHK01_005078"
     CDS             363..1043
                     /locus_tag="ACHK01_005078"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8099853.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1040..1222
                     /locus_tag="ACHK01_005079"
     CDS             1040..1222
                     /locus_tag="ACHK01_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO8099854.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPDLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            1195..1386
                     /locus_tag="ACHK01_005080"
     CDS             1195..1386
                     /locus_tag="ACHK01_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO8099855.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1463..1678
                     /locus_tag="ACHK01_005081"
     CDS             1463..1678
                     /locus_tag="ACHK01_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8099856.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1777..1998
                     /locus_tag="ACHK01_005082"
     CDS             1777..1998
                     /locus_tag="ACHK01_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8099857.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(2209..2811)
                     /locus_tag="ACHK01_005083"
     CDS             complement(2209..2811)
                     /locus_tag="ACHK01_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099858.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            3054..3221
                     /locus_tag="ACHK01_005084"
     CDS             3054..3221
                     /locus_tag="ACHK01_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099859.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
BASE COUNT          993 a          741 c          770 g          785 t
ORIGIN      
        1 ggaccgtggc agtcgcatcc caaacggatg ttacggcata aagccatgat tcagtgtgcc
       61 cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa
      121 aataccgcat acactgcaga acgtcagccg gaacgcgaca tcactccggt taacgatgaa
      181 accatgcagg agattaacac tctgctgatt gccctggata aaacatggga tgacgactta
      241 ttgccgctct gttcccagat atttcgccgc gacattcgag catcgtcaga actgacacag
      301 gccgaagcag tgaaagctct tggattcctg aaacagaaag ccactgagca gaaggtggca
      361 gcatgacacc ggacattatc ctgcagcgta ccgggatcga cgtgagagct gtcgaacagg
      421 gggatgatgc atggcacaaa ttacggctcg gcgtcatcac cgcttcagag gttcacaatg
      481 tgatagcaaa accccgctcc ggaaagaaat ggcctgacat gaaaatgtcc tacttccaca
      541 ccctgcttgc cgaggtttgc accggtgtgg ctccggaagt taacgctaag gcgctggcct
      601 ggggaaaaca gtacgagaac gacgccagag ccctctttga gttcacttcc ggcgtgaatg
      661 ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg caccgcctgc tctcccgatg
      721 gtttatgcag tgacggcaac ggccttgaac tgaaatgccc gtttacctcc cgggatttca
      781 tgaagttccg gctcggtggt ttcgaggcca taaagtcggc ttacatggcc caggtgcagt
      841 acagcatgtg ggtgacgcga aaagatgcct ggtactttgc caactatgac ccgcgcatga
      901 agcgtgaagg cctgcattat gtcgtgattg agcgggatga aaattacatg gcgagttttg
      961 acgagatggt gccggagttc atcgaaaaaa tggacgaggc actggctgaa attggttttg
     1021 tatttgggga gcaatggcga tgaagcatcc tcacgataat atccgggtag gtgcgatcac
     1081 tttcgtctac tccgttacaa agcgaggctg ggtatttccc gacctttctg ttatccaaaa
     1141 tccactgaaa gcccagcggc tggctgagga gataaataat aaacgagggg ctgtatgcac
     1201 aaagcatctc ctgttgagtt aagaacgagc attgagatgg cacatagcct tgctcaaatt
     1261 ggagtcaggt ttgtgccaat accagtagaa acagacgaag aatttcatac gttagccaca
     1321 tccctttcac aaaagctgga aatgatggtg gcgaaagcag aagcagatga gagagaccag
     1381 gtatgacaac cactgaatgc atttttctgg cagcgggctt catattctgt gtgcttatgc
     1441 ttgccgacat ggggcttgtt cagtgacacc tcagcaagaa aacgcccttc gcagcattgc
     1501 ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag tttccggata aaaacgtcga
     1561 tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg gtaacgctga tgggattcac
     1621 accgactcat ttaagcctgg cgatcggcat gttaaacggc gtctttaagg aacggtgagc
     1681 atgaaaagca aaatcatcag ggagctacag gctccttttt tattgttcgc attcaccctc
     1741 aagcgtatta accaacaatt cagggattaa tggaagatgg cagacatcat tgattcagca
     1801 tcagaaattg aagaattaca gcgcaacaca gcaataaaaa tgcgccgcct gaaccaccag
     1861 gctatatctg ccactcattg ttgtgagtgt ggcgatccca tagatgagcg aagacgcctg
     1921 gccgttcagg gttgtcggac ttgtgcaagt tgccaggagg atctggaact tatcagtaaa
     1981 cagagaggtt cgaagtgagc gaaattaact agaagccaaa gataaaatca tcgctgagca
     2041 ggagaaaatc gctaacggag aaaagacagt aagtcagtat atgaaaaccg catgatatca
     2101 tcagataaaa atcgatcgta aagcgaaata ttaataccag aataaacgag tcgaggtaaa
     2161 ttatattacc tcgataaatt aactaaaact tgcccgctat atactatctc attcagtatc
     2221 atcacgcgcg gtctgtgcat atgttactac cgcacctaat gtattaattt tcttttcaac
     2281 atagataata ttatcgtact cataattgcc atacggatag caaatgcgaa tattctcatg
     2341 tagatcgggg tcatccacct cagctccaga acaacttttt gaactaccgg aagtataccg
     2401 atacggtgca acataagacg atgtctctcc aggcaaaaaa taagttagtg ttgtaagggg
     2461 tataatcaga aaaaatccag caaatatgca catccctgca taaaccttaa ggtatgctga
     2521 cagactcttc cagccgcttt gttttactat ccccttctta acccaaaaca gagataacag
     2581 aaaagctatt cccatgctaa acagaatgta atagtgggat atactctgat taagaaacgt
     2641 gaccctgtaa atatctgccc gccaccagaa gaaaaggaaa ataaagatca ggcctgaaac
     2701 tgtcatgcaa atcaaataag gatacgaatc ttttttcatg tttagcgtcc ataaaatttt
     2761 tcctgccccg gacaaattta ccatccattt tttgcgcaga aaatagctca ttacttactg
     2821 cacaataata cacaaaattg cgtaaatttt ttgcatggat tttagctctt tcagccgacg
     2881 tttaaggggt aaatagcatt tcctaaaagc aactgcacca acccaacaga atgagctacc
     2941 gcttacgttg agagcaaaaa agtgtatagc agcaatgaac agcatcctcg cactgacgag
     3001 gatttctttt atctgaactc gctacggcgg gttttgtttt atggagatga taaatgcact
     3061 tccgagtcac aggtgaatgg aatggagaac cattcaacag agttatcgaa gcagagaaca
     3121 tcaatgactg ctatgaccac tggatgatat gggcgcagat agcacatgca gacataacca
     3181 atattcgaat tgaagaactg aaagaacacc aagccgcctg atggcggctt tttcttgcgt
     3241 gtaattgcgg agactttgcg atgtacttga cacttcagga gtggaacgc
//
LOCUS       DAZGCS010000249         3213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGCS010000249 DAZGCS010000000
VERSION     DAZGCS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.252"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /locus_tag="ACHK01_005085"
     CDS             complement(<1..281)
                     /locus_tag="ACHK01_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO8099860.1"
                     /translation="MFGIFGKKARRAAVEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQILRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREI"
     gene            complement(471..680)
                     /locus_tag="ACHK01_005086"
     CDS             complement(471..680)
                     /locus_tag="ACHK01_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099861.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(733..957)
                     /locus_tag="ACHK01_005087"
     CDS             complement(733..957)
                     /locus_tag="ACHK01_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099862.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(1048..1124)
                     /locus_tag="ACHK01_005088"
     tRNA            complement(1048..1124)
                     /locus_tag="ACHK01_005088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:tct)
     gene            complement(1138..1214)
                     /locus_tag="ACHK01_005089"
     tRNA            complement(1138..1214)
                     /locus_tag="ACHK01_005089"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Arg,seq:tcg)
     gene            complement(1222..1297)
                     /locus_tag="ACHK01_005090"
     tRNA            complement(1222..1297)
                     /locus_tag="ACHK01_005090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ile,seq:cat)
     gene            complement(1338..1451)
                     /locus_tag="ACHK01_005091"
                     /pseudo
     CDS             complement(1338..1451)
                     /locus_tag="ACHK01_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1800..2552)
                     /gene="ydfT"
                     /locus_tag="ACHK01_005092"
     CDS             complement(1800..2552)
                     /gene="ydfT"
                     /locus_tag="ACHK01_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8099863.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSSLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            complement(2566..>3213)
                     /locus_tag="ACHK01_005093"
     CDS             complement(2566..>3213)
                     /locus_tag="ACHK01_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099864.1"
                     /translation="QLAQQNLSAWMIDVIRHAMNGIQERELSLAELSWWAVRNQVADA
                     LPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQRTKNIALPSHTHQQQNPPQ
                     EKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQ
                     GGMGTKSHDIFTLPLCREHHNELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
BASE COUNT          813 a          866 c          675 g          859 t
ORIGIN      
        1 tcaatttcac gcaatgccgc tcgacgacca attttaaagt tcgtatccag ctgaccgata
       61 atggttgcgc taatcgcatt aatttctgac gtaaacgcgg acaacgcagg ctggttacgc
      121 aagatctgct cgatcttcgc tttctctgaa gcctcacatt caccatctgc ataggccacc
      181 agataggcag cattaataac cgcctgtgcc agatcacgtt tctcaaactt tttaatttcc
      241 actgccgctc ggcgggcttt tttaccaaaa ataccaaaca tcgtgacgtt cctttgggtg
      301 ggtgagccaa cgcccgggag cgatctgccc acagagaaag tcacactgac cactccgtaa
      361 gctcaccccc gaaaggctct gtggttgata tgcgccgggc gtggcgcgga tacaaaaaag
      421 gccgccaata gcgacctcag ttacgggatt attctggggt taaacgactg ttactccccc
      481 cagacaaaat catcacttcc tgttcgatgc gagccatagt gaacctcgta cttatctccc
      541 atctttcttg cttccgtttc tgcgtcttcc tctgtcgcaa aaaccccaac aagatgccag
      601 ggcgagcttc ttaccacagc ccaaccttta acccatcctt tgttgtcctt atcttccatt
      661 aacacttcag aaacaaacat atttatctcc ttgtgggtac ccagagatat tttatgattg
      721 ctcccggtca gatcaataaa gtggcttcaa ttttgcctta atgatcaaat cagggtgatt
      781 gacggaatcg tacaccacct caatattttc atccgtggcg tcgataagat attcttttac
      841 ataaggacct gttgattttc catgaaatac atcttcaaca agtacactct ccccctgaac
      901 aacacgaaaa ctaacttctg tttcgaacgg accaatcgtc accatcagtt ttttcacata
      961 gcctcctgat aagcactcga tttattagtt aatggtgtaa cgcagataca aaaaaaggcc
     1021 cgcaaaagcg agccaagtaa ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg
     1081 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
     1141 accgccatcg gggacttgaa ccccgcacag ccagcttcga aggctgacgc tctatcccga
     1201 tgagctaatg gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat
     1261 tatgagtcgc tcgctctcac cactgagcta aagggccggg cgcaggataa taacggtacg
     1321 taactaatcc tgcaatatca tccgttctga ctgactaaat cctgaacttc cctgaccgtc
     1381 tgctcaaaac gttcagtctc cagctcaacg ccaattgcac gacgccccag cgacattgct
     1441 gctttgacgc tacagacata aaaaagccag ccactggggg aggctggcaa actcgtagag
     1501 caaaatgctg ttacgcaaac ttcgttacag ggtcatcctg caatacaaaa aatacacaat
     1561 atttagaaaa ctaatagtgc catatgcaat ttttaagatt ttgttattaa ttgtggtcgc
     1621 accttccttt ctgtgtactt tccgtatagc tcacaggatt ctgggtacaa aaaaacccgc
     1681 gcatcggcgg gttcttaaat cttatcaacg gtagacatac aaagcccatc gttgggaaaa
     1741 tcttatccat attttttgaa aaatgcaagc atcatgtcgt catcttcggc gaaaaccatt
     1801 tatcttgtca cctttctcaa ttgtatctct gcatatgctt cttcctgcca gcactttgta
     1861 accagtttat caatgacatc tgcatatcct ttgtaccact gataatccgt caggtctggt
     1921 accagcttct ggacatgaag ccgcgccagt gtggttggta aactgctaaa ccggtttcca
     1981 ttgcaacgcc cacaaacctt ataaacaggc gtgccatgaa gccgggttct tttttcatcc
     2041 aggacaatac ctttaccctt gcaccctctg catgctgtgc tgacttctcc cttaccatga
     2101 caatgctgac atagttcctt cacccactct tccttaataa cagattcccc gcttctggag
     2161 tgtttcacca cctcgcgcaa tacattatga aatccagtac cagcacaatg ctcacagcga
     2221 gccttacttg ccgcagacct ggaataatca gcaaaggcaa aattcacaag gtaaggaatg
     2281 atctgtagcc gggtttcttc actcaattta ttcaatgtcg ggttatccag tgccatcgcg
     2341 taattgagca gaccttcaat cgcaaactga ggatcctgaa caccaacttt tgccaggaat
     2401 aaagcaaacc caagcggtgc ttttgactgc accatcccct gcgcagccat cacatccgta
     2461 attgttaaac caccagagcc tgtcgccggt gcgtcatcgc tcagttttgg agatttcggg
     2521 gagtaatatt tcggtaaggc ttcaaggttc atgctcgttc tccacttacg ccagtacgcc
     2581 aattgccagc gcacgatcga taaaacgaaa tatcagctcc agctgagagc catacttctc
     2641 ttcaaatgcc acggtatccg catgaagctc gttgtgatgc tcccgacaca gcggtagcgt
     2701 gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca ggtgatgggg
     2761 atcgtcggct ggcttaccac aacacgcaca cggctgtgtc tttacccagc gtgtgtattt
     2821 ctcatttacc cagcggcgac gtttaggtcg tttcatgaag gattccggag actccggatc
     2881 aacggcaatg ctgaccaccg tcttttcctg tggtgggttc tgttgctggt gggtgtgaga
     2941 cggtagcgca atattttttg tgcgctgctt cagtatgctg gtggcggtct gttctcccgg
     3001 tatgatgtcg ctttcacggt atacggagcg gattttttcc gcacgcaacc ccagcgaacg
     3061 acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc accaggataa
     3121 ttcagccagc gataattccc gttcctgtat gccattcatt gcgtggcgga tgacgtcaat
     3181 catccatgct gacaggtttt gttgagcaag ttg
//
LOCUS       DAZGCS010000250         3200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGCS010000250 DAZGCS010000000
VERSION     DAZGCS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.253"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1121)
                     /locus_tag="ACHK01_005094"
                     /pseudo
     CDS             complement(1..1121)
                     /locus_tag="ACHK01_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1130..1897)
                     /gene="sctT"
                     /locus_tag="ACHK01_005095"
     CDS             complement(1130..1897)
                     /gene="sctT"
                     /locus_tag="ACHK01_005095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO8099865.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1899..2159)
                     /locus_tag="ACHK01_005096"
     CDS             complement(1899..2159)
                     /locus_tag="ACHK01_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8099866.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIEVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2169..2834)
                     /locus_tag="ACHK01_005097"
     CDS             complement(2169..2834)
                     /locus_tag="ACHK01_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8099867.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2824..>3200)
                     /gene="sctQ"
                     /locus_tag="ACHK01_005098"
     CDS             complement(2824..>3200)
                     /gene="sctQ"
                     /locus_tag="ACHK01_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HFO8099868.1"
                     /translation="ICDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDEQM
                     INSFEELPVKIEFVLGKKIMNLYEIDDLCAKRIISLLPESEKNIEIRVNGALTGYGEL
                     VEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT         1074 a          652 c          486 g          988 t
ORIGIN      
        1 ttaatttttt aaattatcgt ctttattctc gatttttgtg tcttcaccct caatgaattt
       61 atcttctgaa gagagctgtt catcttgaac aggttgccca gcattctcca catcttcaag
      121 ccacagcaga agacgtaata tttcatctat attttcgaag ctaacataat cgtagcgacg
      181 atgggtagca taattttttc gtgctaattt tttatctgtg ataattggta ttccgatttc
      241 ctttgcatat ttccttacgc ctaaggccac ctcattagtt actcttacag aaatcaatgg
      301 aataggtgaa agatgtggct taaagtatat ccctattgca atgtgagtag ggttggaaat
      361 catcaaacga ctattactga catcagactt caattgctca gaaagaattt cctgatgcgc
      421 tctctgcgtt tagacttaat ttctggattt ccttcttgct ccttgtattc tcttttaact
      481 tcttgtttat ccattttcat atctttcata aataaaaaat attcagcgat aaaatcaaaa
      541 attaagacaa ttatcatact gccgagacaa tacagtatga gaaggaatag catctctccc
      601 caatctgata ataaagatat gatatctcca tcaagagttt taaaaagcag tgatttatga
      661 ttactccaaa atactttgat cgccaatgca aaaaaaacaa tataaagaat tgctttaaca
      721 aattctttta cggtttttaa gccaaatata cgctttaagc catttactgg attcaatgaa
      781 tcaaacttaa atttaacagc ttcagttgct aattgcattt tactttgaaa ccaggagaca
      841 agtattgttg tgaacatgca aaccagcaaa aaaggaatgt tcgttttcaa ccaaccaacc
      901 cccatagctt taaaatagtc ccaaatatct ggtttagcgt tatgatcaag gatgtattca
      961 agaatcgaca taatgtgatg cacatcaaaa acatatccga gatataaagt acccacaagc
     1021 attattacag agatggttaa gtctctactt tttagaattt gccctttttt agaagcatcc
     1081 tggagtttct tttgtgttgg cttttcagtt ttattggcca tgaaacactc tatttctcaa
     1141 tgtatagttg caattgttct gggaaaaagg ataacggcat tactcgttca gccagtattg
     1201 gggcaaaata aataattaat attaaaaatg ccagcgcact tttgacagtc agtgaaatgg
     1261 caaaagcatt tagttgtgaa gcataacatg ccagcaatcc caggacagcc tcaccgccca
     1321 gcataacggc aatcacgggg cttgcgtaaa cgatagtatg agtcattatg ttattaatat
     1381 atgagaataa aggctctaat ttaggaaaat taaaattgcc tgaaggccat aaattataac
     1441 tttgccaaag agtttccaga ataaagttca ttccaccttt agtaagataa accgcagcag
     1501 agaaaaggtt aaataatctt gctaattccg aggtatcgac acctgtagcc ggatccaaag
     1561 tactacttaa tgttgcaccg cgttggttat cgataaagct acctatagca aggaacatcc
     1621 aaaacgggga tgctaataag catgcaataa acagccctat gataatttct ctggcagcga
     1681 acaaagcaat atcgatttca tttaagttca ataattcata gtggtaatgc tgaactaatg
     1741 cacatgatac cactattatt attgggtgac gaactactgt tggtatattc ccactggcga
     1801 aaaatggtaa caaatagaaa gttggtgcca atcggcaaaa accgagcgct gtagcagcca
     1861 gcagactatg caactgatag agtatcgcct cccccatgct atcctctcgc gaatgctaaa
     1921 ttcagcattt cgataccaaa tgaatataat ttttcaccat accacccgct cattaaaaaa
     1981 aagcaaatac ttacgcacaa taatttcacg ccaaatggca acgtctgttc ttgcaactgc
     2041 gttaccgttt gaaaaagccc taccaataaa ccaacaaatg tcgcaacttc aataggtcct
     2101 gcagacataa ctaaaatcag atacagcgcc ctattccccg caaaaacaat atcgtccata
     2161 ttttttcctc agggattaat ggataggtca aaatattgta aaatgagccc ttttgataac
     2221 attgtccaac cgtccatcgc gacaaacaaa attaatttta tcggtgtaga tatggttacc
     2281 ggactcatca tcatcatacc taacgttaac agaacgctgg atataaccag atccactacg
     2341 acaaagggta aatagatata aaaacctata ataaatgcac tttttatctc actcaatgca
     2401 tatgcgggca gtaacgagaa aattgaaata ttatcatcat cgattatctc ttcattgtct
     2461 tcagaactat tcaccttctg aatcttttca aaaaagctaa ccaactcagg ttcagaatat
     2521 tttatcaaat atgatttata accactcatg cctgtctcaa caaaattaac aaccgatgct
     2581 acattattga atgaaagatt ctcattctga ctgttattat atatttcctt cccaaccggc
     2641 atcattacga acattgacaa tagcaaagca accccattaa gtgtcatatt tgatggcact
     2701 tgctgaagtc ccagcgcgtt tcggacaata acaaaaacaa tcgagaattt aataaaacat
     2761 gtcccggatg ctattataaa cggcaatagt gtaaataatg ataatatcgc tatcagcgaa
     2821 atgctatttg acattattat tccctgataa ccaagaatga atttctacgc ccaatttatc
     2881 atccacttca acaagttcgc catagccagt tagcgcacca tttacacgta tctctatatt
     2941 tttctcagat tcaggaagca gagatattat tctttttgca cagagatcgt ctatttcata
     3001 tagattcatt attttcttac caagaacaaa ctcaattttt acaggcaact cttcaaaact
     3061 attaatcatc tgttcgtcat tttcatcata aatctcactc tcgttttttt tgatatcaaa
     3121 atcatctgtt tcaaaatctt cctcgtactc aaaatgatca gccattttta actcctctgg
     3181 ataaattaaa tcacaaattt
//
LOCUS       DAZGCS010000251         3196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGCS010000251 DAZGCS010000000
VERSION     DAZGCS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.254"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..600
                     /locus_tag="ACHK01_005099"
     CDS             <1..600
                     /locus_tag="ACHK01_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO8099869.1"
                     /translation="LQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSG
                     ACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFS
                     TAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNN
                     IKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            572..955
                     /gene="yhhH"
                     /locus_tag="ACHK01_005100"
     CDS             572..955
                     /gene="yhhH"
                     /locus_tag="ACHK01_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO8099870.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1060..1902
                     /gene="yibA"
                     /locus_tag="ACHK01_005101"
     CDS             1060..1902
                     /gene="yibA"
                     /locus_tag="ACHK01_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO8099871.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMTLND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1930..2888
                     /locus_tag="ACHK01_005102"
                     /pseudo
     CDS             1930..2888
                     /locus_tag="ACHK01_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2900..>3196
                     /locus_tag="ACHK01_005103"
     CDS             2900..>3196
                     /locus_tag="ACHK01_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO8099872.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVF"
BASE COUNT         1066 a          531 c          732 g          867 t
ORIGIN      
        1 ctgcaggggc gatatatcac tcaggatccg attggactga aggggggatg gaacctgtat
       61 ggatatcaat tgaatccgat atcagacatc gaccccctgg gtttatctat gtgggaggat
      121 gcaaaatcgg gggcatgtac taatggtctt tgcggcacac tatccgctat gataggtcca
      181 gataaatttg attctataga tagcaccgca tatgacgcct taaataaaat aaatagccaa
      241 tctatttgcg aagataaaga gttcgctggt ttaatatgta aggataatag tggcagatat
      301 ttctcaacag cacctaaccg aggagaaaga aaaggatcat atccattcaa tagcccttgc
      361 cctaatggta ctgagaaagt atcagcttat catactcatg gtgcagatag tcatggagaa
      421 tattgggacg aaatattttc aggtaaagat gagaaaatag ttaaaagtaa agataacaat
      481 atcaagtcat tttatttagg tacgcccagt ggtaatttta aagcaataga taaccacggg
      541 aaggaaataa caaacagaaa aggattacct aatgtctgca gagttcatgg taatatgtaa
      601 aaaaatattg tttaggaact gtgtcattgt atctttgttt gtttttacat acaacacatg
      661 ggcgcagtgt aataataata ttaaaataat gcgcaagtat gaaagtgaag gtaaatatac
      721 cgttagaaat ttggttaaaa ataaagctat agcattggaa ttagctgaga tatatgttaa
      781 gaatcgttat ggacaggatg ccgcagaaga agaaaaacca tacgaaatca ctgagttaac
      841 aacaagttgg gttgttgaag gtaccattca ctcagaccaa attgctggtg gggtttttat
      901 tatagaaata ggcaaaaatg atgggagaat tctgaatttt ggccacggaa aataagtatg
      961 taaggataat caaacatcgt gtgcgctgat ggcagagtag gtggaggact ccagacagtc
     1021 aaacgataga aaaagatagc ctttatggag gttcctgcaa tgtcaaatac ataccagaaa
     1081 agaaaggcaa gtaaagagta tggtttatat aatcaatgta agaaactaaa tgatgatgaa
     1141 ttatttcgct tacttgatga tcacaattcc ttgaaaagga tttcatctgc cagagtatta
     1201 cagttaagag gtgggcaaga cgctgttaga ttggcaattg agttctgctc tgataaaaat
     1261 tatatccgta gagatatcgg agcatttata ctcgggcaaa taaaaatttg caaaaaatgc
     1321 gaagataatg tttttaatat tttgaacaat atgacattga atgataagag cgcttgcgtt
     1381 cgagctacgg caatcgagtc aacggctcag cgatgcaaga aaaacccaat ttattcacct
     1441 aaaatagtag aacaatctca aattactgct tttgataaat cgactaatgt cagacgtgct
     1501 acagcatttg ctatttctgt tatcaatgat aaagcaacaa ttccactatt gattaatctg
     1561 ttaaaagatc caaatggaga tgtcagaaac tgggccgcat ttgcaataaa tatcaataaa
     1621 tatgataata gtgatattag ggattgtttt gtggagatgc ttcaggataa aaatgaggaa
     1681 gtccgtattg aagcaataat cggactttcc tacagaaaag ataaaagggt tttatctgtt
     1741 ttatgcgatg agttaaaaaa aaatactgtt tatgatgata tcattgaagc tgcgggtgaa
     1801 ttaggtgata aaacgctact tcctgtttta gatactatgt tgtacaagtt tgatgacaat
     1861 gaaattataa cttccgctat tgataagctg aagcgttcat agcgtgagtt gcctatgcac
     1921 agtgggggat tcccgccggc acggtgcaga tgctcgaccg gctggaaagt gaaatcctgg
     1981 ctgaccgggt gagtgaggaa agccgccgct ggctggcatc gtgcggcctg accgtggagc
     2041 agatgaaaaa ccagatggac ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact
     2101 gcgaccatcg cggcctgccg ctggcgctca tcagcacgga aggggcaaca gcgtggtgcg
     2161 cagaatatga tgaatggggc aacctgctga gtgatgagaa cccgcatcat ctgcagcagc
     2221 tcattcgcct gccgggtcag cagtatgatg aggagtccgg cctgtattac aaccgccacc
     2281 gctattatga cccgctgcag gggcggtata tcactcagga tccgattggg ctgaaggggg
     2341 gatggaattt ttatcagtat ccgttgaatc cggtcataaa tgtagatccg caaggtttgg
     2401 ttgatataaa tttatacccc gaaagtgatc ttatccattc tgtagctgat gagattaata
     2461 tcccaggcgt tttcacaatc ggggggcatg gtacccccac atctattgaa tccgcaacgc
     2521 gcagtatcat gacagctaaa gatctagcat atctaattaa atttgatggg aattataaag
     2581 atgggatgac agtttggtta ttttcttgta atacaggtaa aggacaaaat tcatttgcta
     2641 gccaattagc taaagagtta catacaaatg taataggacc tgacacgcta tggacgtggt
     2701 gggggcgagg aactaatggt aagttaaaaa tggatacagt gctaacagca ccaacgaacc
     2761 ttaattcaaa taaggatcta atggctataa caacaaaaga ccttggtaat tggataacat
     2821 atgggccatc tgggcacccc atttctaata tgcaaggtac gccagaaaaa cccagtgata
     2881 taagataggt tgtagatgta tgaaagcatg cttgttacta tttttttatt tttctcttat
     2941 tggtcaattg catggtgctg atgtgaaaat aaaagaaaac gaaagtgtga tgggatctac
     3001 agcaatgacc tatgatttaa gtgaagaaaa gctgatgaaa ctgaagtata aatcacaaca
     3061 tggtgattca gaggcatcat ttcgactata tcaatattac tgctttacta aaaataatat
     3121 tgataaacaa ttgcgattct tggaaagatc agcatctcag gggaatgtta cagcgcagtt
     3181 taattatggg gtcttt
//
LOCUS       DAZGCS010000252         3148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGCS010000252 DAZGCS010000000
VERSION     DAZGCS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.255"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..280
                     /locus_tag="ACHK01_005104"
     CDS             83..280
                     /locus_tag="ACHK01_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8099873.1"
                     /translation="MLKQQDMTETARAVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            432..1490
                     /locus_tag="ACHK01_005105"
     CDS             432..1490
                     /locus_tag="ACHK01_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8099874.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            1536..1606
                     /locus_tag="ACHK01_005106"
     tRNA            1536..1606
                     /locus_tag="ACHK01_005106"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1564..1566,aa:Met,seq:cat)
     gene            complement(1710..1844)
                     /locus_tag="ACHK01_005107"
                     /pseudo
     CDS             complement(1710..1844)
                     /locus_tag="ACHK01_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2085..>3148
                     /locus_tag="ACHK01_005108"
     CDS             2085..>3148
                     /locus_tag="ACHK01_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8099875.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATRDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWG
                     VDKPLYKDLIGRTKAALKKNPKNVLLAVVWMQGEFDFGGTPVNHAAQFGALVDKFRAD
                     LADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTD
                     ENGVNVPTNKPEEDPDIPGIG"
BASE COUNT          765 a          721 c          933 g          729 t
ORIGIN      
        1 ggtgaatacc ggtctttcag cttgctggct ttttcgacaa gagttattgg tgtgtcacgt
       61 taaccgaaaa agggaaaaag acatgctgaa acagcaggat atgacagaaa ccgccagagc
      121 agtttttaat gaattaagtg ccaccgaacc ggcgacagtc ggggagattg cacagaatac
      181 gtacctttca cgcgaacgct gccagctaat actgacccag cttgttatgg cgggtctggc
      241 agactatcag tttggttgtt acagacgcct tcagtcctga aggctttttt atttgtggta
      301 aatgggcggc tggtgggtgt taggggcact caccagccat ctgctcatgc gttggggtca
      361 caagcaaacc tcaggcccat ctgctttgcg caaaagcggt atgagcctat cagagacagg
      421 cttaatgatc catgtttaat actgtaaaaa tatccagttg tgagttaatc aacgccgact
      481 gcctggaatt tatccggtcg ttacccgaaa attctgttga cctgatagtc acggacccgc
      541 cgtactttaa agtgaagcct gagggctggg ataaccagtg gacgggtgat gaggattacc
      601 tgaaatggct ggaccagtgt ctggcgcagt tctggcgggt gctgaaacct gccggaagtc
      661 tttacctgtt ctgtggccat cgcctggcat ctgacattga aatcatgatg cgtgaacgct
      721 tcagtgtgct gaaccatatt atctgggcga agccgtccgg acgctggaac gggtgcaaca
      781 aggaaagcct gagggcgtat ttccccgcca cagagcgcat tctgttcgcg gaacattatc
      841 aggggccgta tcgtccgaaa gatgccgggt atgaggcgaa gggcagggca ctgaaacagc
      901 atgtgatggc tccgctgatt tcttactttc gtgatgcgcg tgctgccctg gggataacgg
      961 caaaacagat agtggatgcc acaggaaaga aaaacatggt gtcgcactgg ttcagtgcca
     1021 gtcagtggca gctaccgaac gaaagcgatt atctgaaatt acaggcgctg tttgcccggg
     1081 tggcagaaga gaagcatcag cgcggtgaac tggaaaagcc ccaccaccag ctgctggaga
     1141 cgtatacttc actgaaccgg cagtatgcgg aactgcagag tgaatataaa catctgcggc
     1201 ggtattttgg cgtgacggcg caggtgccgt acacggatgt gtggacgcat aaaccggtgc
     1261 agtactatcc cgggaaacat ccgtgcgaaa aaccggcaga aatgctgcag cagataatca
     1321 gtgcgagcag tcgtccgggt gacctggttg cagatttctt catggggtcg ggttcgacag
     1381 tcaaagccgc gatggcgctg gggcgtcgtg caactggcgt tgagctggag actgaacgtt
     1441 ttgagcagac ggtcagggaa gtacaggatt tagtcagtca gaacggatga tattgcagga
     1501 ttagttacgt accgttatta tcctgcgccc ggccctttag ctcagtggtg agagcgagcg
     1561 acgcataatc gccaggtcgc tggttcgaat ccagtacaac gcgccacact tattttccct
     1621 ggctcgcttt tgcgggcctt tttttaaatg tctcacaatt caggcggttg actgttgtct
     1681 ggtttgcggg gagtttgtta aaagaaactg gcatggtgaa tccccctgtg cggaggggca
     1741 atcagcaacc aggtatatgg ggtaatcgcg gattcaggtg ctggtactga attcaccggg
     1801 aggcacccgg caccatgcaa gaaaaagaat gtgcatgcaa acatgcccct ctccggaggg
     1861 gcttttttat gggtaaaaaa atgcccgaat gggttcgggc aatagcatga gatactgata
     1921 ttgttgtgtt gttatcgtgt ggattttaac cagggtttat caggctgcgc aactgcgtgg
     1981 ccttttttca tttcttgggc tgtagtcccc gtgtgtcatt caggcttccg gactacagcc
     2041 cactccatat ctgatttaat acactatccc ggccgggagg aataatgaca tttaaacatt
     2101 atgatgttgt cagggcggcg tcgccgtcag accttgctga tgcacttgcg caaaaaattc
     2161 gtgaaggatg gcaaccatat ggtgggccgt tttcttcgta tacggatgat ggcgcagcac
     2221 ttattcaggc gattgtcgca gaaggtgatg tgagcacacc tgttgtggtg aagccgacag
     2281 gtggagaagg tgcagtaatc agcgccacca gagacccgga gtattacttt gttgtggttc
     2341 tggcggggca gtcaaacagc atggcatatg gtgaaggcct tccgctgccg gagacatatg
     2401 accgtccgga cccgcgtatt aagcagctgg cgcgccgcag tacggtgaca ccgggcggtg
     2461 cagcatgcaa atataacgac atcattccgg cggaccattg tctgcatgat gtgcaggaca
     2521 tgagccgcct taaccatccg aaagcggacc tgtcaaaggg gcagtacgga accgtggggc
     2581 aagggctgca tatcgccaaa aaactgctgc cgtttatacc ggcgaatgcg ggcattctgc
     2641 tggttccgtg ctgtcgtggt ggttcagcgt tcaccaccgg agccgatggc acatacagtg
     2701 acgcgagtgg tgcctcggag aattcaaccc gctggggtgt ggacaagccg ctgtataagg
     2761 accttatcgg tcgaacaaaa gcagcactga agaagaaccc gaaaaatgtg ctgcttgccg
     2821 tggtgtggat gcagggggaa tttgattttg gcggtacgcc ggtaaatcac gcagcacagt
     2881 ttggtgcgct ggttgataaa ttccgtgcag acctggcgga tatggcaggt cagtgcgtcg
     2941 gtggctctgc tggcggtgtt ccctggatat gtggagatac gacgtatttc tggaagcaga
     3001 agaacgaatc cacgtaccag acggtgtacg gcagctacaa aaacaaaacg gaaaagaata
     3061 tccatttcgt accgttcatg acggatgaga acggggtgaa tgtgccgacg aacaaaccgg
     3121 aagaagaccc ggacattccg ggtatcgg
//
LOCUS       DAZGCS010000253         3066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGCS010000253 DAZGCS010000000
VERSION     DAZGCS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.257"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..593
                     /locus_tag="ACHK01_005109"
     CDS             <1..593
                     /locus_tag="ACHK01_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO8099876.1"
                     /translation="YSLIKTEQTGKLLNRKITTTPTPAKLLSYRNADLIKENYITEKV
                     LSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVMDWYNPQKNAFGIMMGTPRR
                     SADIAKEESRNALNFMIMEKDTFNEKILNSNDNLQKKYGTTENSSWVSASVGSLLDKG
                     AKVYPDISCSLRLGEPFIITLPETVRLDVDIHPLKK"
     gene            complement(606..1304)
                     /locus_tag="ACHK01_005110"
     CDS             complement(606..1304)
                     /locus_tag="ACHK01_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099877.1"
                     /translation="MNEIRSNPVKFLEEHLILNPQRYAHHDETSLITVNITREDGLLR
                     LKETEDDMAGSDYILFTGMRDTDSPDRFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQSYSMIHMRPRDSNDFVDEFTPALYANTQEILLER
                     DIQRTLANTFPNEHPEALILVPSAEKFIVDGNSIQLIGIGNELGEFDFYRQIYPVTGG
                     EHLVEALMWTHLPA"
     gene            complement(1505..2086)
                     /locus_tag="ACHK01_005111"
     CDS             complement(1505..2086)
                     /locus_tag="ACHK01_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO8099878.1"
                     /translation="MPLTSDINSSSFHLGMEVLRAQVAATGRGEFTMGGETVRIEYSP
                     TDGRFLASDGTGGLFTELLLLGFNNGPQALGERMLSILSGSDAGETQSQVTPQDKIYQ
                     CKFSVNTESLQCPSDATRCPIILETPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSV
                     HPLTRAPITPSMIVKPEECKYDPARGSFIIKDS"
     gene            complement(2155..2292)
                     /locus_tag="ACHK01_005112"
                     /pseudo
     CDS             complement(2155..2292)
                     /locus_tag="ACHK01_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            complement(2513..2662)
                     /locus_tag="ACHK01_005113"
                     /pseudo
     CDS             complement(2513..2662)
                     /locus_tag="ACHK01_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2980..>3066)
                     /locus_tag="ACHK01_005114"
     CDS             complement(2980..>3066)
                     /locus_tag="ACHK01_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8099879.1"
                     /translation="DKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          939 a          738 c          527 g          862 t
ORIGIN      
        1 catattctct aataaaaaca gagcaaacag gaaaactgtt aaacagaaaa ataaccacca
       61 ctcccacacc agcgaaacta ttatcatatc gaaatgcaga tttaataaaa gaaaactaca
      121 ttacagaaaa agttttatct attttcaata taaaacggga tttcgttgct gtaagaatcc
      181 aaagcaatca gtttactgat ttaaaaaaca agacaattca gggtcataaa gacactgtcg
      241 caaaagtcat ggactggtat aacccacaaa aaaacgcatt tggtatcatg atgggcacac
      301 caagacgttc tgctgatata gcaaaggaag aatccagaaa cgctctaaat ttcatgataa
      361 tggaaaagga tacatttaat gaaaagatac ttaacagcaa tgataattta caaaaaaaat
      421 acggcacaac agaaaatagt agttgggtat cggcttctgt tggttctctt ctggacaaag
      481 gagccaaagt atatccagat atatcatgct ctctgagact gggagaacca ttcattatta
      541 cacttccaga aacagtcagg ctggatgttg atatccaccc actcaaaaaa tgaacctgaa
      601 cgttttcatg caggcaaatg cgtccacatc aaagcttcaa ccaggtgctc tcctcctgtc
      661 acaggataaa tttgtctgta aaaatcaaac tctcccaact cattaccaat accaatcagt
      721 tgaatgctgt tgccatctac aataaatttt tccgctgacg gaaccaaaat taaggcttcc
      781 ggatgttcat taggaaaggt gttagcaaga gtacgctgaa tatccctctc cagcaaaatc
      841 tcctgagtat tagcgtaaag cgctggcgta aactcatcga cgaaatcgtt actatccctt
      901 gggcgcatat gtatcattga atacgactgt tctccagatt ctgataatcc atggcgaaca
      961 ataagaacac tacagccgct ttgctgattg gttagccata acggtgaact ttgctggata
     1021 gtttgagaaa tatcatttcg ccggaggctt atacactgat tactcaagct tacaactgca
     1081 ggtgcatcaa agcggtccgg actatcagta tcacgcatac ctgtaaacag aatataatca
     1141 ctaccagcca tatcatcttc cgtttctttt agtcttaaca agccatcctc tcttgtaata
     1201 ttaactgtaa ttaaagatgt ctcatcatga tgggcatagc gctgtggatt aagaattaaa
     1261 tgctcttcca gaaattttac agggttacta cgtatttcat tcattataca ccgaagctct
     1321 gaacgatgcg gcaccagaaa gtgcccggta ttttgtgaaa taatactact taccttgatt
     1381 gttttctctc cagctcatga aaaataactc gcgtattata ttgttttacc aaccacaggc
     1441 cccgaatgcc tgtggtgttt ttatataaat tatcggaagt taatattgtt tcatttgaaa
     1501 catcttaact atcttttata ataaagcttc cccttgcagg gtcatattta cactcttctg
     1561 gtttaacaat cattgatggg gttattggag ctcgtgtcag agggtgaact gaaccgtcat
     1621 taaccacgcg agaaagtgca tcaacatcaa ataaagtgca tactgctgaa ctatctgaat
     1681 ttttgacaaa taccccttct tctggtgttt ccagtataat tgggcatcga gttgcatcag
     1741 agggacactg gaggctctct gtattaacag aaaacttaca ttgatatatt ttatcctgag
     1801 gagtcacttg cgattgtgtt tcacctgcat ctgatcctga aagaatactt aacattctct
     1861 cgccaagagc ttgaggccca ttattgaacc ctaaaagcaa taattcagta aataatcccc
     1921 cagtgccatc gctggccaga aagcgcccat ctgttgggct atattcaatt ctgacagttt
     1981 caccacccat tgtaaattct ccacgcccag tggctgcaac ttgagcacga agaacctcca
     2041 ttccaagatg gaacgaagat gaattaatat ctgaggttaa tggcataata aagctccctg
     2101 ttttaatttg aactccagac ttaaaggtcc acaacaaatg tcatccacaa atttttccct
     2161 cgtcccatca gcacaccgaa tttgcagcgg cctcaccacg cactgtatcg gctttttatc
     2221 cgcacccagt atcaccacct gcgtgattac cctgtcctgc tccggaataa tgccctattc
     2281 gcttgttctc agaaattgcc agcaacctta cactacttct tttgagccca tttttcgcca
     2341 gccctgaata caggaaagtg ttaactgttt ttatgtttac cctcatctca ctggcgagat
     2401 gggacggacg caactgacgg tagatatatc caaacatcac caccatctct gacatcgtca
     2461 gtgcccgtgc ttttttgttc caggtccaca atctgttacg cacagcatta tgtatatcat
     2521 gtcccccttt caacgcttcc agatccagct gaataaaacg gcgtccctga aacatgctgc
     2581 gcattaaatg gcccaactga ctatcacaga ttattaacca ggattcttta ctatccagaa
     2641 taaccctgcg attttccggc ataaaaaatt cttgagccag aattacacgg catcctttca
     2701 gtaacaaacg caaaccatat tccagataat aattacgcgt cgcaagaatg agcttcatcg
     2761 ccctacttcc ctgaactaca gtagtgttcc atgcctcggt cgcaatcaga atttatgatt
     2821 ccccctccag ggaacaagat ggacactccc ctgctcctta aaggcatcct tcactccatc
     2881 gtattaacat gtttattact tcacactcta ataacaaaca tcccccatac atgacaacaa
     2941 aaaccggagc cggcctccgg ttttttgtga acccgtcggc tatttcatcc cgccaatatt
     3001 ttcccacgtc ccgtcagcac gcaggatttg cagcggtctt accacacact gtatctgctt
     3061 tttatc
//
LOCUS       DAZGCS010000254         3063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGCS010000254 DAZGCS010000000
VERSION     DAZGCS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.258"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHK01_005115"
     CDS             <1..112
                     /locus_tag="ACHK01_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8099880.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHK01_005116"
     CDS             171..374
                     /locus_tag="ACHK01_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO8099881.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..1424
                     /locus_tag="ACHK01_005117"
                     /pseudo
     CDS             374..1424
                     /locus_tag="ACHK01_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1541)
                     /locus_tag="ACHK01_005118"
     tRNA            complement(1452..1541)
                     /locus_tag="ACHK01_005118"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Ser,seq:cga)
     gene            1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHK01_005119"
     CDS             1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHK01_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO8099882.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2533..2608
                     /locus_tag="ACHK01_005120"
     tRNA            2533..2608
                     /locus_tag="ACHK01_005120"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2566..2568,aa:Asn,seq:gtt)
BASE COUNT          893 a          651 c          701 g          818 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaaaggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
     1321 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
     1381 aagaaaatat tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag
     1441 tgattgaatt ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg
     1501 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
     1561 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
     1621 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
     1681 cccttccctg gcaggaagca ctatcgatcc cccttttaac gtgtctgacg gaacaggaac
     1741 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
     1801 agggctttga actggattca ttaagaagct gccggatagc acttctattt tgcctacccg
     1861 ttctggagtt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1921 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1981 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     2041 attcttttga tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     2101 cccgtaacgg cgatcgcgcc agcggagttc cctttattcc gttgcgtgag gttgctggct
     2161 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg
     2221 agaatgcggc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     2281 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     2341 aacgtttctg ccaattttat caacaagatc ctttgcagag actgcatcgc actaatgata
     2401 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     2461 ttgaaatgac ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgccc
     2521 cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     2581 actggttcga gtccagtcag aggagccaaa ttctaaaaat tcgctttttt agcgcaatgt
     2641 cactgacctt agttgaacat tgttttttaa cggatagcgg gtttttaaca tcttaagcgc
     2701 cctcgacctt tatggttgag ggcgttttgc tatgaacgcc atcaccattt tcccctcgat
     2761 tataaaactt gagttattca gtagtctccc ctcttgcaac tcacacccaa aactgcctaa
     2821 cgaaaagtta ttaattttca atcatattgc tatcaggatt tacatttttt cgctgtgcta
     2881 gaaagggcgc atttatgtta gctcgttcag ggaaggtaag catggctacg aagaagagaa
     2941 gtggagaaga aataaatgac cgacaaatct tatgcgggat gggaattaaa ctacgccgct
     3001 taactgcggg tatctgcctg ataactcaac ttgcgttccc tatggctgcg gcagcacaag
     3061 gtg
//
LOCUS       DAZGCS010000255         3059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGCS010000255 DAZGCS010000000
VERSION     DAZGCS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.259"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK01_005121"
     CDS             complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK01_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor YmfM"
                     /protein_id="HFO8099883.1"
                     /translation="MNSLTTHYRRSQLIALPVPGGKAKVEYCYAVNVPGDREIVTHSF
                     AEWAVGDFNRQKETVLCDKLTAGSKITTE"
     gene            complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK01_005122"
     CDS             complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK01_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO8099884.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(806..1006)
                     /locus_tag="ACHK01_005123"
     CDS             complement(806..1006)
                     /locus_tag="ACHK01_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8099885.1"
                     /translation="MTTDDIESYFGSIEKVAAFFGITTEAVYQWRNRPGQLIPKGRAA
                     EAAYRTCGRLPFKPELYEKSNG"
     gene            1104..1730
                     /locus_tag="ACHK01_005124"
     CDS             1104..1730
                     /locus_tag="ACHK01_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8099886.1"
                     /translation="METVGQRIKALRRVTGTSQKELGKFCGVSDVAVGYWEKDINTPG
                     GEALSKLAKFFNTSIDYILYGAEFEGKLVTNMRRVPVISWVQAGQFTECRAAEVFSEV
                     DKWVDTSLKIGDNSFALEVKGDSMTNPNGLPTIPEGATVIVDPDAEPRHGKIVIARLD
                     GTNEATVKKLVIDGPQKFLVPLNPRYPNIPINGNCLIIGVVKGVQYEL"
     gene            1937..2221
                     /locus_tag="ACHK01_005125"
     CDS             1937..2221
                     /locus_tag="ACHK01_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099887.1"
                     /translation="MKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIMAGVVFSL
                     RRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQLFPDFFR"
     gene            2484..2794
                     /locus_tag="ACHK01_005126"
                     /pseudo
     CDS             2484..2794
                     /locus_tag="ACHK01_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2809..>3059
                     /gene="yfdP"
                     /locus_tag="ACHK01_005127"
     CDS             2809..>3059
                     /gene="yfdP"
                     /locus_tag="ACHK01_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO8099888.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTET"
BASE COUNT          838 a          706 c          709 g          806 t
ORIGIN      
        1 actccgtagt gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc
       61 ggttgaaatc acccacagcc cactcagcaa agctgtgggt tacaatttcc ctgtcacctg
      121 gtacattaac tgcatagcaa tactccacct tcgcttttcc acccggtaca ggaagcgcaa
      181 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacagtc
      241 acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt ctgaaatgca
      301 aaagattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga gatacagctc
      361 gtcgttaatt gctgtcttct catgcggttc caccacaccg tcttcgattg ccgaacgaat
      421 ctgtctggaa taactgccaa tctgttcaat gacttccagc aggcgctggt taatatcggc
      481 gttgtccaca tcctcgacgt caggaagaga cacaaagacg ccatttgcag actgcgccac
      541 agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc atcccagcgg
      601 gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttttctg ttacatccag
      661 ccagtcagct gcttcagcgt aaccacccgg caacgctgcg atagtttttc tgacagcttt
      721 cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg ttagcctcat
      781 cgttctgtgg tttctgttaa tcgatttatc cattagattt ttcataaagc tcaggtttaa
      841 atggcaaccg tccgcaagtt ctatatgcag cttctgctgc acgtcctttt ggaattaact
      901 ggcccggacg gtttcgccac tgataaacgg cttcagttgt tatgccgaaa aaagcagcaa
      961 ctttctcaat actgccgaag tagctttcga tatcgtcagt tgtcatacgc cctccaaact
     1021 aagttttatt agatgctaat tacaaatcta tctttggtca ataaaaacta agattactta
     1081 gcaattcaag aaatggtgct cctatggaaa cggttggtca gcgtataaaa gctctgagaa
     1141 gagttaccgg aacgtcccag aaagaattgg gtaaattttg tggagtaagc gacgttgctg
     1201 tggggtactg ggagaaagac atcaataccc ctggtgggga ggcactttcg aaattagcga
     1261 agttcttcaa tacgtcaata gattacattc tttatggtgc tgagtttgaa ggcaaactcg
     1321 tcacaaacat gcgcagagtt cctgtaatat cgtgggttca ggctgggcag tttactgagt
     1381 gcagggcagc agaagtgttt agtgaagtgg acaagtgggt agatacatca ttaaagattg
     1441 gtgataactc atttgcatta gaggttaaag gtgactccat gactaaccct aatggcctcc
     1501 caacaatacc agaaggcgca acagtgattg tagatccaga tgcagaacct cgtcatggaa
     1561 aaatagtcat cgctcgactt gatggaacaa acgaagctac agtaaaaaaa ttagtcatcg
     1621 atggccctca aaagttttta gtgccattaa atcctcggta tcccaacatc cctatcaatg
     1681 gtaattgcct tatcattggt gtagtcaaag gagttcaata cgaactctaa gacctctctt
     1741 ctctaactaa ggcaccgaac taagaaaagt ttggtgtttt ctcttgccat aataactaag
     1801 ttaagttaga ttttatatca aagataacga acaggcagga cgcccacgaa gtagccgcct
     1861 ggggcatatg aagtccagga tgattcgttg agtcatgttg tgccatcagg cactcatgtt
     1921 aaagcaggtg tatgaaatga aagtccagat tttaaacaat aactgtgaag tcgtttggtc
     1981 atacgacata gccgcccctg tagatcagag cggcgatagc tggaccaatg ggaaacatca
     2041 gattatggct ggagttgtgt tctctttacg ccgtgctttg gaacaggctg aagtatttcc
     2101 atcagaccct gaatggaaat ggcctttttc tatttgtcca aattcggaga gcacatttca
     2161 gaaaattggt cagaaagtcg cactcgaaga gcatcaactg tttcctgatt ttttcaggta
     2221 actcgtcggc atcgcagaaa caacaacgct cgatcatgtt gaaagccgat tcgtagaacc
     2281 gtttctgctg agtgtcgctg agacaggaaa agagtgacgt tacgatgatt ttattaattg
     2341 cattatcaag ttctttttca tcaaaagtca tttgattttc cttttatgta tacgggctta
     2401 aaaggatacc accgagcctg aagtggtgaa aagacaggca cataacagct aagtattttc
     2461 aaccaaagag aatccttagc gttgtggtga atgcggctca gcgcacgcgg gttaaggttg
     2521 aggctgacag tcgaccttct gtggataccc acccgtctgg tgtgcaacct tcgccaggca
     2581 ccgggaggca cccggcacca caactttatg ctgtgtgtag tcctggcggt accagtttgt
     2641 acccttgctt ccggctggta gcgtcctttt tacaaaacag agaagagcat caccggacga
     2701 cgggctcata acccaatcca tccgggcggc tgccaccgca ggtgttcttc tctgttttgt
     2761 ggagaaacta atcggccttg cagggtcgat atgatgagga gcagcaaaat ggctagcgaa
     2821 cgcagtactg atgtgcaggc atttatcggg gagctggacg gcggcgtatt tgaaaccaaa
     2881 atcggcgcag ttctcagtga agtcgcttcc ggtgtgatga acacgaaaac caaaggtaag
     2941 gtctcactca acctggaaat cgaaccattt gatgagaacc gtgtgaaaat caaacacaaa
     3001 ctctcatatg ttcgcccgac taaccgcggg aaaatttctg aagaagacac caccgaaac
//
LOCUS       DAZGCS010000256         3023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGCS010000256 DAZGCS010000000
VERSION     DAZGCS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.260"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..1132)
                     /locus_tag="ACHK01_005128"
     CDS             complement(50..1132)
                     /locus_tag="ACHK01_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO8099889.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLP
                     VSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            complement(1204..1629)
                     /locus_tag="ACHK01_005129"
     CDS             complement(1204..1629)
                     /locus_tag="ACHK01_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8099890.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(1613..1855)
                     /locus_tag="ACHK01_005130"
     CDS             complement(1613..1855)
                     /locus_tag="ACHK01_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8099891.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            2247..2585
                     /locus_tag="ACHK01_005131"
     CDS             2247..2585
                     /locus_tag="ACHK01_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8099892.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            2878..>3023
                     /locus_tag="ACHK01_005132"
     CDS             2878..>3023
                     /locus_tag="ACHK01_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8099893.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNS"
BASE COUNT          728 a          812 c          672 g          811 t
ORIGIN      
        1 tgcgaatacg ttcaagaacc ccggctgccg caatgttttt catgccacat caccccctga
       61 atcccggcgg aatttcggta tccggttcag aaatatgatt cacacaacgc tggttgttcg
      121 tgccgcttac cgggagcaac caggggtttt caaaattccg gtccggccca aaaaacgtcg
      181 tcgctcgctg aacaaattcc gttcccgttt tcccggtagc cgccaggtat cttgcgtaac
      241 gcctcacacc atccagtatg gcctctggtg gcaccccctc gcgtaatctg gccttccagg
      301 cactgaaagc ggatttcttc gggtttgccc cagcacgcaa cgggtattcc cgccagacct
      361 gttcgaacac atccggataa tccactcgtc ccacaggctg cccggtgttt tccgggacta
      421 cccgatcggc ttcccgctga atggcggaat cggcttcagg ctgctgcagt tggtgtgatt
      481 gctccggcct tgcggtcatc acctgctgca cagcgcccga atcggctttc agcgcatacg
      541 ctgaatcggc ttccggtgtc gtgcctgctg gctgaccagg attgacggtc tgaacatccc
      601 ctgcctggtt cgtggcgttt tttacgccat ggaccatagt gttttgatct tcttgatctg
      661 tatctttatc tgtatcttta tctgtatctt tatctgtcgt gactcgtcgt gacatgtgcg
      721 tgacatttcg tgacgcgccg tgacaatcgc cattttgttc ccgctttctt tccctctctc
      781 gctgcgccct cttgcgctct gcaggagatt ttgcggtttg cgaaatattg ccgttgtcct
      841 ctttaagcac ctggcgtttt tcccatccag tgattaaatc accatcaagt acccgcccct
      901 gcatcgtctg caaaattgaa tcaattacct cttctgtcac gtcgagcgca cttgccaaat
      961 cttctgtcgt gacatcaatg tgacctcgcg tgacatttcg tgacgcgctc accaggaggt
     1021 ggatatacac tgccatcact gttgcaattg gctgccctga caccctggca attgttcgcc
     1081 acttagggtc atttggcatg tcatgccata atctgagcca ggcgttagcc atactcacct
     1141 cttctgatac cgaatctttt tactcacgag ttgccggaag cgattcgata cggctattgt
     1201 cagtcaatgt actgccacag catttcctgc cgggccacca cggttcatct gattgaaacc
     1261 ggcgattgcc actgcgacaa aatcatcagc gtctctcacc agtcgctccc gcgtctccac
     1321 cagctcccga aaataagctg aactgtggct gcgcattctg gccaccagca aaggtggcat
     1381 tgccttttcg atcgctggta acaacgcctg aattttttca actgcatcag gggtgtcttt
     1441 ctctacccag cggaaaattt tctgggtatt gcgagccagg gcttccggat ggctgtcgtc
     1501 atacagttca ggaaacgtca tacccaactc aaaataagcc tgggttattc cagctgccgg
     1561 aactttttcg ccatcaggac gcgcccaggc attcatcgcc atgcggatgt gttcatgctt
     1621 gattttcatg aatcccccct tggttagaag gcggattatg atcagaaccg ggaatgacaa
     1681 ccgtcggtat gtgtaactca tatttgagcg ccccggcagt gactgcctga attagcaacg
     1741 cccatttcca cggaacctct tccccccaca tgctgactgt ggtttttgac gttcctagag
     1801 ctgcggctgt tttaacaact ccgccaaaat agcctaatac ttctgatttt ttcatgagtc
     1861 gctccataaa actgaacgcc aaaagtttaa taatcaaaac caaagaaagt caagaaacaa
     1921 aaccatctgt gttttaaaat caaaacatga gcaagcaaac aatatctgaa cgcataaccc
     1981 aacgtatgca tgcgctaaac ctgaaaggca aagaccttgt caatgccact ggcgcatcaa
     2041 aaggctccgt aagtcaatgg atgaacggtg gaggagcgcc gtcctcgcgt tacataagtt
     2101 cactggcaaa aatattgaaa gtaaacgaaa attggcttct taatggagga gagttaaata
     2161 caggtgattc gcttgatcta tctttaccgc cgataaaaac ggttccgcta ctatcacttc
     2221 agcaggcagc aagctggagt gattatatga aaaattcctc aataacctct tgtgtgcagc
     2281 ttgtcggaga aatcccggcc aatacctttg cagttgttct agagagtgac agtatgtcaa
     2341 catctggtgg gggagtttcc atcccaaatg gttcaacagt ttttgttgat cccaatcgaa
     2401 ccgtacaacc aggaaatatt gtccttgcct tacccaaagg gaccacaaca cctgtcattc
     2461 gtaaactgga gatagaaggg ccggatattc ttttagtccc cacgaatcct cgctaccctt
     2521 caattatgct ggatgatcta tcttgcatat tgggcgtatg ctttaaaatt caacaagata
     2581 tttaaccgac ctcatctatt tgattaactg tatgccatcg tggtgatggc ttaacagctg
     2641 cctgcttaaa atgttttgat aaaaaaacat tgacctgaaa agttcatttt tctaaacttc
     2701 attcattccc tctccccacc ccacagaatg cagggcaata cttcgagtta ccaggcagtg
     2761 gtcaggggtt aagtagccag cccgaggcgt aagaacatga cggcagggtt caactttaat
     2821 aactatgcag caggtttttg ttccgctacc ccggcgttaa ggggaaatga ggtcagcatg
     2881 gatactatcg atcttggcaa cagcgaatct ctggtatgtg gcgtgtttcc caaccaggac
     2941 ggtacgttta ccgcgatgac gtataccaaa agtaaaacgt ttaaaaccga agctggcgcg
     3001 catcgctggt tagcaagaaa ctc
//
LOCUS       DAZGCS010000257         2883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGCS010000257 DAZGCS010000000
VERSION     DAZGCS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.262"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            442..630
                     /gene="dicB"
                     /locus_tag="ACHK01_005133"
     CDS             442..630
                     /gene="dicB"
                     /locus_tag="ACHK01_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8099894.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            627..818
                     /locus_tag="ACHK01_005134"
     CDS             627..818
                     /locus_tag="ACHK01_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8099895.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            911..>2883
                     /locus_tag="ACHK01_005135"
     CDS             911..>2883
                     /locus_tag="ACHK01_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO8099896.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIICEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NTMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNSTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT          860 a          672 c          712 g          639 t
ORIGIN      
        1 gcataacgcg aaagcgtacg gcgaagctct ttcccttaga aggcttgtcg ttagatttct
       61 tcgaacgtgc gcttccggtt gtggcactcc gcgaaatggc gcggcggtaa gtatggcggg
      121 gttattcctt ccccgttgag gacaccgggt tgtcaggttg acaatacgct taagtgacaa
      181 ccccgctgca acgccctctg ttatcaattt tctggtgacg tttggcggta tcagttttac
      241 tccgtggctg ctctgccgcc ctttttaaag tgaattttgt gatgcggtga atgcggctaa
      301 gcgcacgcgg aacagttaaa acaagcggtc ttttacgggc gtaacgggaa tgctctgtat
      361 ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg catcacaaaa
      421 ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac gtctgaaggt
      481 tgttttgaaa ttggtgtcac tatcagtaac cctgtattta ctgaagatgc cattaacaag
      541 agaaaacacg aacgggagct attaaataaa atatgcattc tttcaatgct ggcccgttta
      601 cgtccgatac aaaaaggatg ctggcaatga atacagcatt tgcacttgtt ctgacagttt
      661 ttcttgtttc cggagagcca gttgatattg cagtcagtgt tcacaggaca atgcaggagt
      721 gtgtaactgc agcaaccgaa cagaaaattc ccggtaactg ttacccggtc gataaagtta
      781 ttcaccagga taataacgaa atcccggcag gtctttaaaa cagttccgta ataaacatcc
      841 gatttcattc ttatatgcca gcaatggcag ggatttgttc acccttaaat ctgtaatgag
      901 gtaaaacaaa atgagtaaag tctttatttg cgccgccatt ccggacgaac aggcaataaa
      961 ggaagaaggt gcagtcgctg tagccacagc tattgaagct ggcgacgaac gccgtgctcg
     1021 agcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact gcgcttataa
     1081 atttattatc tgcgaggata aacctggcat accccgccct gccctcgatt catgggatgc
     1141 tgaatatatg caggaaaacc gctgggatga ggagtctgct tcctttgttc cggttgagac
     1201 tgaatcagat ccgatgaacg tcacttttga caagctggcc cctgaagtac agaacgctgt
     1261 catggttaag ttcgacacat gtgaaaacat caccgttgat atggttatta gcgcacagga
     1321 attgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt tgatgaaaat
     1381 gccagaagtt aacaccatgt atccggagct taagttgcac gccattgggt gggttaagca
     1441 taaatgtatt cctggtgcta aatggcccga aattcaggca gagatgcgca tctggaaaaa
     1501 acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg atctcgcccg
     1561 cgccagaacc aatcaacagc acactgaaaa ttcaacagga aaaatcagcc cggtcattgc
     1621 tgccattcat cgcgaataca agcagacatg gaaaacactg gatgacgaac tggcctacgc
     1681 tctctggcct ggtgatgtgg atgccggaaa cattgacggc agcatccatc gctgggcaaa
     1741 aaatgaagtt atcgacaacg accgcgaaga ctggaagcgt atctcggcat caatgcgcaa
     1801 acagcctgat gcccttcgct acgaccgcca gactattttt ggccttgtcc gtgaacgtcc
     1861 gatcgacatt cacaaagacc ctgtggcact gaacaaatac attactgaat acctgactac
     1921 aaagggcgtg tttgaagatg aaggaacaaa tcagagcgca actgatactc tctcgtcgcc
     1981 agtaccagaa actgatgcag tggaaacggc aattccggac aacgaaaaaa ccgaatgcaa
     2041 agtggaagtc gaaccatctg tagagcgtga ggggccgttc tacttcctct tcaccgacaa
     2101 ggatggcgaa aaatacggtc gcgcaaacaa actttctggt ctggataagg cactggctgc
     2161 cggggctact gaaatcacga aagaagaata tttcgcccgc aaaaacagta catactcagg
     2221 ttcacaacaa aatactggtg catctgacac gaccgcacaa ccagagccgg taaaagttac
     2281 cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg acgccgataa
     2341 gttgcttgct gcctctcgcg gagaatttgt tgcagggatt agcgacccga atgatccgaa
     2401 atgggttaag gggatccaga cccgcgattc tgtaaaccag aaccagcatg aatcggaacg
     2461 gaactaccaa aaagcggaac aaaacagccc aaatgcgtta caaaacgagc cagaaacgaa
     2521 acagcctgaa ccagtggcgc aacaggaagt ggaaaaagtc tgcaccgcct gcggtcagac
     2581 cggcggcggc aactgccctg attgtggcgc agtgatgggc gacgcaacat accaggaaac
     2641 attcgatgaa gagtatcagg ttgaagttca ggaagatgat ccggagaaaa tggaaggcgc
     2701 tgaacatcca cacaaggaga acactggcgg caatcagcat cacgatagcg ataatgaaac
     2761 tggcgagacg gcagatcact caattaaggt gaacggtcat caagaaatca catccaccag
     2821 caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc ctgatgcccc
     2881 gat
//
LOCUS       DAZGCS010000258         2819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGCS010000258 DAZGCS010000000
VERSION     DAZGCS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.263"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(66..141)
                     /locus_tag="ACHK01_005136"
     tRNA            complement(66..141)
                     /locus_tag="ACHK01_005136"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Ile,seq:cat)
     gene            complement(281..835)
                     /locus_tag="ACHK01_005137"
     CDS             complement(281..835)
                     /locus_tag="ACHK01_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO8099897.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(832..1122)
                     /locus_tag="ACHK01_005138"
     CDS             complement(832..1122)
                     /locus_tag="ACHK01_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO8099898.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(1122..1721)
                     /locus_tag="ACHK01_005139"
     CDS             complement(1122..1721)
                     /locus_tag="ACHK01_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO8099899.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
     gene            2221..>2819
                     /locus_tag="ACHK01_005140"
     CDS             2221..>2819
                     /locus_tag="ACHK01_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8099900.1"
                     /translation="MTENTTPTADFSPSKRFFVSMLTRDIDLNDAILDLLDNCVDGAL
                     RTIKDTKKTSKPYEGFYAKLTINKDVFIIEDNCGGIPKSFREYAFKMGRPHQKEEENE
                     GTVGVYGIGMKRAIFKMGRDCSIQSNNPDGAFTVDITPDWIDGDGWKIPMHESDYDNK
                     NPTGTTIEIKKLHSNVAQKFNESTYLTDLFLQIKHSLSF"
BASE COUNT          796 a          679 c          575 g          769 t
ORIGIN      
        1 ccccgcacca acaaccctgt tatggtgtcg tctgctcttc ctgataagct aatggcggtt
       61 tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc
      121 tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta attccgcaat
      181 aaaaaacccg ctcaatggcg ggttctggta aagttcatgc gtttggttcg cctcgcgata
      241 cagttttgcg aagcttaccg gaattgaagc agtttttacg tcaaaaagca ataacttttt
      301 tctctatacc aaaagccata accattggtt tgtacaaaat aaattctgcc acctttagcc
      361 aatgctcaat gcgtctttca caggttctta aactccattc cggatgtgca tcattcagca
      421 gttcagccat tttgcgctta gtcatccccc gccccacata acgctgactc agaacattga
      481 gcagcccggg ataacctgcc agtacttcac caataaccct gtcgattatt aacgcctctg
      541 aatcggtaca atgcaccagc cagctttttt gcttgccgtt gatcatatcc cgcaaaaaag
      601 cctcaagttt aggtttgtcc agacctgctt ttttcatcct ccggagcgcc tcgttaattg
      661 ccgtttttgt cagcttttta gaggtcagca actggttgaa catatttccc gtcttaccgc
      721 cgccaatata cgaccagcgc ccccacatac gtagtttccc ctgaatccag acactttcca
      781 gcgtggtgag acgaaggtgc tctccgcttt ttcctgtgtt cgttgggtaa atcataaata
      841 accgtccttt ctccagatct cttgtgtgcg aaaaacacct tctgcatgca tcaggcgcaa
      901 ttcttctttg gtgtaatcgc tggtttttac tctcccgtcg attaaatcgt ggcatgagtt
      961 acaggcaatc gctgcctgca tatcgtgtgg ctttatcgct gttccgcacg ttcccgccag
     1021 cctgtaatgc gccagcacag aggtttcggg attgtgattg cagtagccag ggattctgat
     1081 ctggcatatc tggcctttag ccgctttacg taaattcacc attacgcaaa ctccagtagt
     1141 tgtgcggcca cattttcaac ttcctcctga gaggagaatt tacggaacag aatccagttc
     1201 cacagcacat tcagtacaga tttataaacc tgctgaaact cgacttcgtc catattcgca
     1261 aaagcgatgg attttgcccg acgcccacgg ctaccgtccg gataaatatg ctcggtgtaa
     1321 aatccggcct gaatggttac ccactcgcgg aaagcctcaa acgactttag caacgccgta
     1381 tcccgggttc tacgagtcgc aacggtgtta aggtattgct ctgcggcatc actcagggct
     1441 ggcgtgtgtt cccgaccaac tgattcgcac aggtaatcaa cgaaaccaga cagcagttct
     1501 cgttcgcgag gcgtgatcgc cccaccgacc ggagtccagt aatcgaatcc cagttgcagg
     1561 agtttgaaaa aacgcttgtg gaatgcgtag ttacgcacac gcttaaaatc tgcgtgtatc
     1621 cactcaccta ttttgatttg atgcagaaaa tcgcaactct ccggcgtcgc cgggagaagt
     1681 aaaccagaag aagtttgttt gaccagttgt atatgcgcca tttctcaatc tctcgatggc
     1741 gcagtgcagc agatgccagt tgttcaagct agcacgcaaa ttgtaaacca gaatgccagg
     1801 aaaaaacaaa acccgccgaa gctggttgtg tgcggttgcg ttgaagtctc ctagccaaag
     1861 agtagcggtg accactcccc gccgataatc ttagttgttt aatttcgtaa gctaactagg
     1921 ctaaaagctc tctgttgtct gcctgttgta caattaacaa aatgaacaac gaactagata
     1981 taccaacgcc agaatggtgc aacaaatccc aaacaacaca gcaatgcaac aataacatat
     2041 gttattttcc tctcacggga ttcagtcctg aattctcata aagctaatct ttttttaaaa
     2101 acatactgtt agatcacatg cactttcttt cttaacatcg ttgacttcgc atgcgaagcg
     2161 tttaaaatca acaatttgca cttaaggtat ttattttccc aaacacatag agagtaaatt
     2221 atgactgaga acacgactcc aacagcagac tttagtccta gtaaaagatt ttttgtaagt
     2281 atgctaactc gagatatcga cttaaacgat gccatacttg atctgcttga taactgcgtc
     2341 gatggggctt tacgtacaat aaaagacaca aaaaaaactt caaaaccata tgaaggattt
     2401 tatgcgaaac ttacgattaa taaagatgtc tttattatag aagacaattg cggaggtatc
     2461 cctaaaagct tccgagaata tgcttttaaa atgggacgac cccatcaaaa agaagaagaa
     2521 aatgaaggca ccgttggcgt ctatggtatt ggtatgaaaa gagccatttt taaaatgggt
     2581 agagattgct caatacaatc gaataatccc gatggcgcat tcactgtaga tataacacct
     2641 gattggatcg atggtgatgg ttggaaaata ccgatgcacg agagtgacta tgataacaaa
     2701 aatccaacag gcacaactat agagataaaa aaactccatt ctaatgtggc acaaaaattt
     2761 aatgagagca catatcttac tgatctcttt ttacagataa aacactcatt gtcatttat
//
LOCUS       DAZGCS010000259         2802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGCS010000259 DAZGCS010000000
VERSION     DAZGCS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.264"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(7..843)
                     /locus_tag="ACHK01_005141"
     CDS             complement(7..843)
                     /locus_tag="ACHK01_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO8099901.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(843..1646)
                     /locus_tag="ACHK01_005142"
                     /note="aph(3'')-Ib"
     CDS             complement(843..1646)
                     /locus_tag="ACHK01_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO8099902.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1707..2522)
                     /gene="sul2"
                     /locus_tag="ACHK01_005143"
     CDS             complement(1707..2522)
                     /gene="sul2"
                     /locus_tag="ACHK01_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO8099903.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          678 a          792 c          771 g          561 t
ORIGIN      
        1 tgatatctag tatgacgtct gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg
       61 ttgctcctct tctccatccg cgttccaagc tgcggaaagg cacccataag cgtacgcctg
      121 gtcgagcagg cgacgcggat cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc
      181 aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc
      241 ggcgccaaag cccacttcac cgaccagacc gacgggatct atcaccagcc agccgcgact
      301 ggagaacatg atgttttcat gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt
      361 gctcatcatt tgatcggcta taatcgccgc gtggacgtag tcagtttgac aacctgcgtt
      421 ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc
      481 agaaggcagg ggttcctcag atgcggcata cagcttcgcc attagttccg ctgcaatttc
      541 ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag agcattcgct ccccggcata
      601 ttcgagcaac atcagattgt tctcacgacc gagcaaccgg actgctcccc tcccattgcg
      661 ccataccaga tagtcggccc cgcgcagttc atcagcaatg tcttctatag gtttcaatcc
      721 cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt
      781 gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa
      841 catcaacccc aagtcagagg gtccaatcgc agatagaagg caaggcgttc gcggtcgggg
      901 gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc gctctgcttc atctggcgct
      961 gcccagttct cttcggcgtt agcaatcatg agtgccaaat cggcatagcg atctgctgtt
     1021 ccgagccgcc caaggtcgat cagacccgtg cattgaagag ttttagggtc caccatgaag
     1081 ttcggcatgc agggatcacc atggcaaaca accatatcgg tgcgctcttg gtcgagccgc
     1141 accggtagct ctcgttcgac acgagccaaa agatcgagct gcggcgtact cttgtcctcg
     1201 tccggtaaga agtcgggatt gacggcattg cgggacacca catcaacggc gcgtccgaac
     1261 attcgcgaca gcctgcgctc aaacggacat tgatcaaccg ataggctgtg aacagcgcca
     1321 agttgctgcc ccattgacgg ccacgctttg agcaaatccg ctccagacag atcagccgcc
     1381 ggtactcccg gaattgccgt tatcaccaag catgcaccct cctgttcctc ctgccagttg
     1441 atgacctcgg ggcaagccac acctcgacct ttgagccaaa tgaggcggtc acgctctcca
     1501 gcgagctcac cgcggcggga agcaggtgcg attttcgcga aggcatgccc gtcaccacgt
     1561 cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca accagtcaga atgcgattca
     1621 ccaaaaaaaa tattagttcg attcaatgga ggttccttca gttttctgat gaagcgcgaa
     1681 tatagagaaa tatcccgaat gtgcagttaa cgaattcttg cggtttcttt cagcgccgcc
     1741 aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg tgcggatgaa gtcagctcca
     1801 cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc cgacatcccc cggaccacgg
     1861 cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca gaagcaccgg caaatcgaag
     1921 cgcagccgca attcatcgaa ccgcgccagc accgagagcg aggtttcggg agcagccccc
     1981 agaaaaaacc ccatgccggg atcaaggaca aggcggttgc gtttgatacc ggcacccgtc
     2041 agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat ccatgatgtc gccagcgggt
     2101 gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca taacgacgag tttggcagat
     2161 gatttcgcca attgcggata gaacgcagcg tctggaaaac cgcgaatatc attgagatag
     2221 gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt gataactgtc gagcgagacg
     2281 ggaatgccat ctgccttgag cgcgtccagc accggcgcga tacgcgcgat ttctgtgtcg
     2341 gacgaaacag gcgcggcgtc gggattgctg gatgccggac cgaggtcgat cacatctgcc
     2401 ccctcggcca tcagcttacg cgcctgcgca atggctgcgt ctggcgccag ataccggcct
     2461 ccatcggaga aactgtccga ggttatgttg acgatgccga aaatgatgag cgatttattc
     2521 atgggggctt ctataataat aataatcgag catgagtatc atacggatgc tcgggtcgaa
     2581 agggaatccc caggcgagta acctgtttgc ggtgatccat tagctgcagg agcagaagag
     2641 catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca gcgctcagta
     2701 ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca tggtattacc
     2761 aattagcagg aaaataagcc attgaatata aaagataaaa at
//
LOCUS       DAZGCS010000260         2773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGCS010000260 DAZGCS010000000
VERSION     DAZGCS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.265"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1155
                     /locus_tag="ACHK01_005144"
     CDS             join(<1..236,236..1155)
                     /locus_tag="ACHK01_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8099904.1"
                     /translation="FTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRE
                     RQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERFTIAARLSD
                     SHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEM
                     LTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQFAVPEPDRV
                     WCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAYETRGQPRD
                     VVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKTEWVPTNGY
                     AGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT"
     gene            1165..1716
                     /locus_tag="ACHK01_005145"
                     /pseudo
     CDS             1165..1716
                     /locus_tag="ACHK01_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            1716..1961
                     /locus_tag="ACHK01_005146"
     CDS             1716..1961
                     /locus_tag="ACHK01_005146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099905.1"
                     /translation="MQHVSEGVIQVPELPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQQNIIHP"
     gene            2055..2528
                     /locus_tag="ACHK01_005147"
     CDS             2055..2528
                     /locus_tag="ACHK01_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8099906.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDENWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2544..>2773
                     /locus_tag="ACHK01_005148"
     CDS             2544..>2773
                     /locus_tag="ACHK01_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO8099907.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETL"
BASE COUNT          663 a          745 c          765 g          600 t
ORIGIN      
        1 cttcacccct gagttcaggc tggaatgtgc acagctgatt gttgataagg gctactcata
       61 tcgacaggcc agtgaagcga tgaatgtcgg ttcaaccacg cttgagagct gggtacgcca
      121 gctcaggcga gagcgccagg gtattacgcc ctctgccaca cccattactc cagaccagca
      181 acgtatccgc gagctggaaa agcaagttcg ccgtctggag gaacaaaata cgatattaaa
      241 aaaggctacc gcgctcttaa tgtccgactc gctgaacggt tcacgatagc cgccagacta
      301 agtgacagcc acacggttgt cagcctgtgt tctgctctgg aaatacaccg cagcagttac
      361 cggtactggc gaaaacgacg cgatacggtt aatccggcac gagtcaggtt gtgcagcgaa
      421 atacgccggg cgtggaacca aagtcggggc tctgcgggcg cgcgcactct ggctgaaatg
      481 ctgactcaaa acggcgtccc gatgagccgt taccgtgccg gacgtctgat gaaatatctg
      541 aacctgagca gttgtcagcc cggaaaacat cagtataaaa atgcccgtca ggagcatacc
      601 agcctgccga atctgcttga gcgtcagttc gcagtaccgg agccagaccg ggtatggtgt
      661 ggagatatta cgtatctctg ggcaggaaat cgctggtgct atctggcggt tgttatggat
      721 ctttttgccc gcagggttat cggctggagt ctgtcagcgc atgccgatac cgcactgata
      781 agcagtgccc tgcggatggc ctatgagacg cgtggccaac cccgggatgt cgtgttccat
      841 agcgaccagg gaagccagta tacaggcctt aaatatcaac aacttctctg gcgttgcaga
      901 ataaatcaaa gcgtcagtcg gcggggaaac tgctgggata acagccccat ggaacgcttc
      961 ttccgcagtc tgaaaacaga atgggtgcca acgaatggtt acgcaggcaa ggacgaggcc
     1021 cggcagcaaa ttaatgatta catattgaac tactacaaca gcgtcagacc tcaccattat
     1081 aacggtgggc tgacgccgga agagtcagag aacagatacc atttttactg taaaaccgtg
     1141 gccaatatta cttgaccact acacggagag ataaacggag aacatgtccc tgaaattatt
     1201 ctgctcaact ctcatgacgg tacctccagc taccagatgc tgccgggtta cttcaggttc
     1261 gtctgccaga acggatgcgt ctgtggtcag tctctggggg aagtgcgtgt tccgcaccgg
     1321 ggaaatgtgg tggacagagt cattgaaggg gcttacgagg tggtgggcgt gtttgaccgg
     1381 atagaggaga agcgtgatgc catgcagtcg ctgctcttac cgccaccggc acgccaggcg
     1441 ctggcacagg cggcactgac ttaccgttat ggtgatgaac atcagcccgt caccactgcc
     1501 gacattctga caccacgacg ccgggaggat tacggtcagg acctgtggag tgcttatcag
     1561 accattcagg agaatatgct gaaaggcgga atttccggtc gcagtgccag aggaaaacgt
     1621 atccacaccc gtgccattca cagcatcgac accgatatta agctcaaccg cgcattgtgg
     1681 gtaatggcag aaacgctgct ggagagcctg cgctgatgca gcatgtctct gaaggcgtga
     1741 ttcaggtacc agaattaccc gacgccccgg aggcgctgcc ggggtgtcgt tccatttctg
     1801 atgatgacct gtgcgcctgg tgtacccgtc tgctttaccg tcccggagaa ctcagtctgt
     1861 gcagactgag tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt
     1921 acggctgtcc ggaatttcag cagaatatca ttcatccctg atattaccgg gtctcacggt
     1981 cagtgatgaa cgtgatgttg ccagctcccg acctttactc cctctcattt attcacatta
     2041 caaggatttc atacatgaaa acattgtctc agaacaccac ctcttcggcc tgtgcaccgg
     2101 agactggcct gcaacaactg gttgccacca tagtccctga tgaacagcgc atcagcttct
     2161 ggccgcagca ctttggcctc attccacagt gggtgaccct ggagccccgt atcttcggct
     2221 ggatggaccg tctttgcgaa gactactgcg gtggtatctg gaatctgtac acactgaaca
     2281 acggtggcgc atttatggcg cccgaaccgg atgacgatga tgacgaaaac tgggtactgt
     2341 tcaatgccat gaacggtaac cgcgctgaaa tgagcccgga agccgccggc attgccgcct
     2401 gtctgatgac gtacagtcat catgcctgtc gtacggagtg ttatgccatg acggtccatt
     2461 attaccggtt gcgggattac gccctgcagc atccggaatg cagcgccatt atgcgcatca
     2521 tcgactgaat ggagagaagc acaatgcaac aactttcctt tctgcccgga gagatgacgc
     2581 ccggcgagcg cagtctcatt ctgcgggccc tgaaaaccct ggaccgccat cttcatgaac
     2641 ccggcgtggc cttcacctcc acgcatgcgg cacgggaatg gctgattctg aacatggcgg
     2701 gactggagcg tgaagaattc cgggtgctgt atctgaataa ccagaatcag ctgattgccg
     2761 gtgaaaccct ctt
//
LOCUS       DAZGCS010000261         2770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGCS010000261 DAZGCS010000000
VERSION     DAZGCS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.266"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2751
                     /gene="eae"
                     /locus_tag="ACHK01_005149"
     CDS             <1..2751
                     /gene="eae"
                     /locus_tag="ACHK01_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type epsilon"
                     /protein_id="HFO8099908.1"
                     /translation="VNQNSFANGENYFKLSSDSKLLTQNAAQDRLFYTLKTGETVANI
                     SKSQGISLSVIWSLNKHLYSSESEMMKAGPGQQIILPLKKLSVEYSALPVLGSAPVVA
                     AGGVAGHTNKMTKMSPDATKSNTTDDKALNYAAQQAASLGSQLQSRSLNGDYAKDTAL
                     GMASSQASSQLQAWLQHYGTAEVNLQSGNNFDGSSLDFLLPFYDSENMLAFGQVGARY
                     IDSRFTANLGAGQRFFLPENMLGYNVFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYF
                     RMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLMYEQYYGDNVALFNSDKL
                     QSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKPWSQQIEPQYV
                     NELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTEHSTQKIQLIVKSKYGL
                     DRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNIYKVTARAYDRNGNSSN
                     NVQLTITVLPNGQVVDQVGVTDFTADKTSAKADNVDTITYTATVKKNGVAQANAPVTF
                     SIVSGTATLGANSAKTDGNGKATVTLKSGTPGQVVVSAKTAEMTSPLNASAVIFVDQT
                     KASITEIKADKTTAKANGSDAITYIVKVMKNNQPEANHSVTFSTNFGNLGGNSNTQIV
                     KTDKDGRATVKLTSGVAGNAVVSAKVSEVNTEVKAPEVKFFSVLSIDSNVSIIGTSAN
                     GALPNIWLRYGQFKLTAKGGDGKYQWRSQDPSVASVDALTGRVTLLKKGTTTIEVVSG
                     DNQTAMYTINTPTKFISVETQNKVVYSDAEATCRMNNARLPSSTSELKDVYNKWGAAN
                     SYEGYKGKKTITAWTQQTEDDKQKGWTSTFDIVTKNEIPSNGSNSKVHVNKANAFAVC
                     VR"
BASE COUNT          833 a          531 c          654 g          752 t
ORIGIN      
        1 gttaaccaga attcatttgc aaatggtgaa aattatttta aattgagttc agattcaaaa
       61 ctgttaactc aaaatgccgc tcaggatcgc cttttttata cgttaaaaac aggtgaaact
      121 gttgccaata tttctaaatc acaggggatc agtttatcgg taatttggtc actgaataaa
      181 catttataca gctccgaaag cgaaatgatg aaggctgggc ctggtcagca gatcattttg
      241 ccactcaaaa aactgtctgt tgaatatagt gccttacctg tcttaggttc ggcacctgtt
      301 gttgctgcag gtggtgtcgc tggtcatacg aataaaatga ctaaaatgtc cccggacgcg
      361 actaaaagca acacgaccga tgacaaggct ctaaattatg cggcacaaca ggccgcgagc
      421 cttggtagcc agctccagtc gcgctcactg aacggcgatt acgcgaaaga taccgctctt
      481 ggtatggcca gcagccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg
      541 gcagaggtta atctgcagag tggtaataac tttgacggta gttcactgga cttcttatta
      601 ccgttctatg attccgaaaa catgctggca tttggtcagg tcggggcgcg ttacattgac
      661 tcccgcttta cggcaaattt aggtgctggc cagcgttttt tccttcctga aaatatgttg
      721 ggctataacg tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt
      781 ggcgaatact ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc
      841 tggcatgagt catacaataa gaaagactat gatgagcgcc cggcaaatgg ttttgatatc
      901 cgctttaatg gctatttacc atcatatccg gcattaggcg ccaaactgat gtacgaacag
      961 tattatggtg ataatgttgc tttgtttaat tccgataagt tgcagtcgaa tcctggcgcg
     1021 gcgaccgttg gtgtaaacta cactccgatt cctctggtga cgatggggat cgattaccgt
     1081 catggtacgg gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat
     1141 aaaccgtggt ctcagcaaat cgagccacag tatgttaacg agttaagaac attatcgggc
     1201 agccgttacg atctggttca gcgtaataac aatattattc tggagtacaa aaagcaggat
     1261 attctttctc tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt
     1321 caattgatcg ttaagagcaa atatggtctg gatcgtatcg tctgggatga tagtgcatta
     1381 cgcagtcagg gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct
     1441 attttgcctg cttatgtgca aggtggcagc aatatttata aagtgaccgc tcgcgcctat
     1501 gaccgaaatg gtaatagttc taataatgta cagctcacta ttaccgtttt accgaatggg
     1561 caggttgtgg accaggttgg ggtaacggac tttacggctg ataagacatc ggctaaagcg
     1621 gataacgttg ataccattac ttataccgcg acggttaaaa agaatggtgt agctcaggct
     1681 aatgcccctg taacatttag tattgtatcc gggactgcaa ctctcggggc aaatagtgcc
     1741 aaaacggatg gtaacggtaa ggcaaccgta acgttgaagt cgggtacgcc agggcaggtc
     1801 gtcgtgtctg ctaaaaccgc ggagatgact tcgccactta atgccagtgc ggttatattt
     1861 gttgatcaaa ccaaggccag cattactgag attaaggctg ataaaacaac agcgaaggca
     1921 aatggttctg atgcgattac ctatattgtt aaagtaatga agaataacca accagaagca
     1981 aaccattctg ttacattctc aacgaacttt ggtaatctgg ggggaaattc taatacccaa
     2041 attgtgaaaa cggataaaga tggtagggct acggtaaaac tgacatctgg cgttgcaggt
     2101 aatgctgttg ttagtgcaaa agtcagcgaa gttaatacag aggttaaggc tcctgaggta
     2161 aaattcttct cagttctgag cattgatagt aatgtgagta ttattggaac ctccgctaat
     2221 ggcgctttac ctaatatttg gttgcgatat ggtcagttta agctgacagc caaaggtggc
     2281 gatgggaaat atcaatggcg ctctcaagat ccaagtgttg catcagttga tgctttaact
     2341 ggtcgagtta ctttgctgaa gaaaggaaca acaacaattg aagttgtatc gggtgataac
     2401 caaacagcaa tgtatacaat taatacacct acaaaattta tatctgtgga gacacaaaat
     2461 aaagtagtct atagtgatgc tgaggcaaca tgtagaatga ataatgcacg cttgccgtca
     2521 tctacgagtg agctaaagga tgtgtataat aaatggggcg ccgccaatag ttatgaaggc
     2581 tataaaggta aaaaaacaat aacagcatgg acacagcaaa ctgaggatga taaacaaaaa
     2641 ggttggacta gtacatttga catagttaca aaaaatgaaa tccctagtaa tggcagtaat
     2701 agtaaagtcc acgtgaataa agctaacgct tttgccgtct gtgtaagatg atcttataat
     2761 cattaattaa
//
LOCUS       DAZGCS010000262         2715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGCS010000262 DAZGCS010000000
VERSION     DAZGCS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.267"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            468..683
                     /locus_tag="ACHK01_005150"
     CDS             468..683
                     /locus_tag="ACHK01_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8099909.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            683..1180
                     /gene="rrrD"
                     /locus_tag="ACHK01_005151"
     CDS             683..1180
                     /gene="rrrD"
                     /locus_tag="ACHK01_005151"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO8099910.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1177..1614
                     /locus_tag="ACHK01_005152"
     CDS             1177..1614
                     /locus_tag="ACHK01_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8099911.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDRITAQVNYLQEYIRTQCLK"
     gene            1764..2381
                     /locus_tag="ACHK01_005153"
     CDS             1764..2381
                     /locus_tag="ACHK01_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8099912.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAIMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2569..>2715)
                     /locus_tag="ACHK01_005154"
     CDS             complement(2569..>2715)
                     /locus_tag="ACHK01_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8099913.1"
                     /translation="FPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEKRAYTSIQ
                     SDDG"
BASE COUNT          735 a          600 c          698 g          682 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc
      661 gtaaggctgc gagaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat
      721 tggtggtggg gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg
      781 tctggaaggt gtcagctaca taccatacaa agatatcgtt ggcgtatgga ctgtatgtca
      841 cggacacacc ggaaaagaca tcatgcccgg taaaacgtat accgaagcag aatgcaaagc
      901 cctcctgaat aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaacgtcga
      961 tataccggaa acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggcaa
     1021 tttcagaaca tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaaa gcgcatgtga
     1081 tcagctacgg cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg
     1141 tgagattgag cgtgaagtct gtttgtgggg gcaacaatga gcagagtaac cgcgattatc
     1201 tacgttctgg tcatctgcct catcgtctgc ctttcatggg ctgttaatca ttaccgtgat
     1261 aacgccatcg cctacaaaga gcagcgcgat aaagccacat ccatcatcgc tgatatgcag
     1321 aagcggcaac gtgatgtagc agaacttgac gccagataca caaaggagct tgctgatgct
     1381 aatgcgacta tcgaaagtct ccgtgctgat gtttctgctg ggcgtaagcg cctgcaagtc
     1441 tccgccacct gtgcaaagtc aacgaccgga gccagcagca tgggcgatgg agaaagccca
     1501 ggacttacag cagatgctga actcaattat taccgtctcc gaggtggaat cgacaggata
     1561 accgcgcagg ttaactacct gcaggaatac atcaggacgc agtgcttaaa ataattttaa
     1621 tttcactgaa atttaacaag tgactttcag gaaaatgcct cgcagatgcg gggcattttt
     1681 gtaccggtat ttcaccgcgc accgcagcgc acaataaaca ccgaacctga ccctttggaa
     1741 tgggcctttg aggataccag ttagtgctgg cgagcctcgg tgggctggtt tcctgtgcgg
     1801 caaaggttca tttcaaagaa gcaggcaacg ccatgaatga attaattgcg aatcatgact
     1861 tcgactttcg ccagttagtt accgcagcag aaggtcaacc ggtaactgac accttccaga
     1921 ttgccagggc atttggtaaa cgccatcagc atgtgattag ggctattaaa tgtttgagat
     1981 gttctgagga attctcgaca acccattttt gggccgtcga gaaaatcaat gacttaggga
     2041 tttttgacaa gaaacagatt tactaccgca tggactttag tggcttcgtt atgctggtta
     2101 tgggatttaa cggggcaaaa gccgatgctg ttaaagaagc ctatatcaat gcgtttaact
     2161 ggatgtcagc agaactccgt aagtacagcg aaagttatga agcagaacgc aacgccataa
     2221 tgctggagta tatgaaagag aaggatgtcg ccagcatgtc aggccgtctg ctcaatcgct
     2281 gggggagaac gaaaaaacct caattgcttg caaagctgga acgtctggag agacagggac
     2341 agtttttatt accgggattc gataaaggta ttcaagcctg acacattatg cgctgtatcg
     2401 tcgccgtatt cccgcattaa ccatgaccgt agcccgacgg ggaattcctt ctgcgtgagt
     2461 gtgcgggaat aatcaaaaac gatgcacacc gggttttact gtgctgacag acgcagggtt
     2521 accctcatag tcgcttttcc ggtgcgatgg tggaagaaac cgggatgttc atccatcatc
     2581 actttggatt gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat
     2641 gacccaggct gagaaattcc cggacccttt ttgctcaaga gcgatgttaa tttgttcaat
     2701 catctggttt ggaaa
//
LOCUS       DAZGCS010000263         2709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGCS010000263 DAZGCS010000000
VERSION     DAZGCS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.268"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..765
                     /gene="paa"
                     /locus_tag="ACHK01_005155"
     CDS             7..765
                     /gene="paa"
                     /locus_tag="ACHK01_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO8099914.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPEADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1104..1259
                     /gene="hokD"
                     /locus_tag="ACHK01_005156"
     CDS             1104..1259
                     /gene="hokD"
                     /locus_tag="ACHK01_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO8099915.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1480..1740
                     /locus_tag="ACHK01_005157"
     CDS             1480..1740
                     /locus_tag="ACHK01_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099916.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKDAGIKAGFSAFEDE"
     gene            1807..2085
                     /locus_tag="ACHK01_005158"
     CDS             1807..2085
                     /locus_tag="ACHK01_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099917.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHDPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2087..>2709
                     /locus_tag="ACHK01_005159"
     CDS             2087..>2709
                     /locus_tag="ACHK01_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099918.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMQVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRVEKIRSLYRESDIVPG"
BASE COUNT          715 a          628 c          711 g          655 t
ORIGIN      
        1 ttttttatga ggaacataat ggcaggtttt ttaatattcc tgtcttctgc tgcttatgct
       61 gatatcaatc tgtatggtcc tggtggcccg catacagcct tgcttgatgc agccaaactt
      121 tatgccgaaa aaacaggtat tatagtgaac gttcattacg gcccacagaa caaatggaat
      181 gaagatgcca aaaaaaatgc agatatcttg tttggcgcat cagaacaatc tgctctggct
      241 atcattcggg accataaaga cagcttcagt gaaaaagata ttcagcctct ttatctgcga
      301 aaaagtattt tactggtaaa gaaaggtaat cctaaaaata tccggagtat tgacgacctg
      361 accagacccg ggattggcgt aattgttaat gatggtggtg gtaccagtaa tacatcaggc
      421 actggcgtct gggaagatat tgccggacgt aaagggaata tagaaactgt cgccgcaatc
      481 cgaaaaaata ttattttata tgcgcccaat agcggaactg cacgtaaggc tcttgagaat
      541 cagcctgaag cagatgtctg gataacctgg gctgactggg cagccagtaa tccagaaatt
      601 ggtgatgtcg tggaaatagc gccagactac gtgatatggc gtgatatgaa cattacagta
      661 cgtcaggatg caaatgatga aacccgtcga tttgcagaat ggctacaaac cgatgaagcg
      721 gcgcctgcat tcaaaaaata tggctggacc aggaaaggca cttgacatcc tccacgtcct
      781 tcaggacgtg gattcttttt ccggatgccg cgccagcggc atgtaggggc agctcacaaa
      841 acggaaaaaa ttgtacgcta agcctcgcca ggtgaactga attcattccg atatgggaat
      901 tcccatatcg gacgaaaacg gcttgctgta acggcagagt taagtagaat tgctgcgggt
      961 gcttgaggct atctgcctcg ggcatgaaca ccaacggcag atagataaaa gccccacccg
     1021 actataaatc gaagtgaggc ccctatatgc tcgtcacata tagattgcct cttacggacc
     1081 gaaaggtcaa ggagaagcag gctatgaagc agcaaaaggc gatgttaatc gccctgatcg
     1141 tcatctgttt aaccgtcata gtgacggcac tggtaacgag gaaagacctc tgcgaggtac
     1201 gaatccgaac cggccagacg gaggtcgctg tcttcacagc ttacgaacct gaggagtaag
     1261 agaccaggcg ggggagaaat ccctcgccac ctctgatgtg tcaggcatcc tcaacgcacc
     1321 cgcacttaac ccgcttcggc gggtttttgt ttttattttc aacgcgtttg aagttccgga
     1381 cggcgccgga atagaatcaa aaatacttaa gtagcgcgca gggagaagag ggatggaccc
     1441 cgaacagggg agtgctattt atctggaagg attctgttga tgaaaatcga agaattacgt
     1501 gaaattttta gtgaagatgg cctctatact gtgcgcgttg agaatggcgc tattgtcagc
     1561 cactgccgta ttaaatgttt acagtctcaa caaaggaaga gtggagctgc gttaattcat
     1621 tttgtggatg ggcttgtgac ggatggtttt attttgcgtg caaatgaatt tgtcacatcg
     1681 ttgccgtctc tgaaagacgc tgggattaag gctggttttt ctgcttttga agatgagtga
     1741 attcatctac aattcagcgc agggctgaac ccctgttgag taacactgtg ccaccggaga
     1801 aagccgatgg cgcaaaattc cagactacac aattctgata attcagccgt ctttgccagc
     1861 aggcacgggc ggcgttctca tgcattcaaa tctgactggt tccggcacga cccatgcact
     1921 gaagaacagg ccgaatggct gattcagaac taccgcagac gtgggtatga gtttaggaaa
     1981 gccctcagcc tcgattatcg tcactggata atctccgtca ggcttcctta ctccgaacgc
     2041 ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaacgtgc gggtattact
     2101 tcgacctgtt ctggtaccgg aactcgggct ggtggtcctt aagccaggtc gtgaatccat
     2161 gcaggtattt cacaataccc gggtactggt ggagccggaa ccgaaaagca tgcgtaatct
     2221 gccgtccggg gtcgttcctg ccgttcgcca gccgctggtg gaagacaaaa cattgctgcc
     2281 atttttcagc gacgaacgag tgattcgtgc tgctggtggt gctggtgcat tgtctgactg
     2341 gctgttgcgc catattaaat cctgccagtg gccacacggc gattatcacc acagcgaaac
     2401 tgtcattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg acaaccagct
     2461 acgggaccag acatccgaat cactcgagca acttgctcat caaaacctgt cagcatggat
     2521 gattgacgtc atcggtcacg caataagcgg tacgcaggag cgtgaattat ctctggctga
     2581 attatcctgg tgggcggtct gcaatcaggt ggcggacgcg ctaccggagg ccgtattacg
     2641 tcgttctctg gggttacgtg tggaaaaaat ccgctccttg taccgcgaaa gcgacatcgt
     2701 accgggaga
//
LOCUS       DAZGCS010000264         2701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGCS010000264 DAZGCS010000000
VERSION     DAZGCS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.269"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..999
                     /gene="ompT"
                     /locus_tag="ACHK01_005160"
     CDS             46..999
                     /gene="ompT"
                     /locus_tag="ACHK01_005160"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO8099919.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1186..2670
                     /locus_tag="ACHK01_005161"
     CDS             1186..2670
                     /locus_tag="ACHK01_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8099920.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHYLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          864 a          578 c          553 g          706 t
ORIGIN      
        1 aaaaatacat attcaatcat taaaacgatt gaatggagaa cttttatgcg ggcgaaactt
       61 ctgggaatag ttctgacaac ccctattgcg atcagctctt ttgcttctac cgagactata
      121 tcgtttactc ctgacaacat aaatgcggac attagtcttg gaactctgag cggaaaaaca
      181 aaagagcgcg tttatctagc tgaagaagga ggccgaaaag tcagtcaact cgactggaaa
      241 ttcaataacg ctgcaattat taaaggtgca attaattggg atttgatgcc ccagatatct
      301 attggggctg cgggctggac tactctcggc agccgaggtg gcaatatggt cgatcaggac
      361 tggatggatt ccagtaaccc cggaacctgg acagatgaaa gtaggcaccc tgatacacaa
      421 ctcaattatg ccaacgaatt tgatctgaat atcaaaggct ggctcctcaa cgaacccaat
      481 taccgcctgg gactcatggc cggatatcag gaaagccgtt atagctttac agccagaggt
      541 ggttcctata tctacagttc tgaggaggga ttcagagatg atatcggctc cttcccgaat
      601 ggagaaagag caatcggcta caaacaacgt tttaaaatgc cctacattgg cttgactgga
      661 agttatcgtt atgaagattt tgagctaggt ggcacattta aatacagcgg ctgggtggaa
      721 gcatctgata acgatgagca ctatgaccca ggaaaaagaa tcacttatcg cagtaaagtc
      781 aaagaccaaa attactattc tgttgcagtc aatgcaggtt attacgtaac acctaacgca
      841 aaagtttatg ttgaaggcgc atggaatcgg gttacgaata aaaaaggaaa tacttcactt
      901 tatgatcaca atgataacac ttcagactac agcaaaaatg gtgcaggcat agaaaactat
      961 aacttcatca ctactgctgg tcttaagtac accttttaac gaagttaacc agattttctc
     1021 cccggtggta ttgtataacc ccggggaatt tgtttacagt tacaagtagg caatatccac
     1081 taattttgta agcatttaac ctatgtcata acgtgtaatc tcttaccgca acacaagacc
     1141 tcaatatatt caaatcttat ctttattccg atattcagag acacaatgcg aaaaataatt
     1201 actcatttca aagttgtttt aacgttactt ctaccagtaa ccgtatctgc ccagcagata
     1261 cagtggcaat cctgtatggc cagtcaattc aaccactggt ttggtgagga aaaaccgtct
     1321 cctgacttac tatgtggtta tttgtctgtt ccattaaaat atacagacac aggcggagat
     1381 gcttcttatg aaaaaaaatc acaagtcaaa ctagcgttga caaaattgcc ggcaaaaagc
     1441 aagcataaag gaagtatcct gataataagt ggtggtcccg ggttaccagg cataaatcct
     1501 tatattaact ttgactggcc agtcacaaat cttcgtgagt catgggatat tattggattt
     1561 gatcctcgag gcgtcggaca gtccactccg acaataaact gccggcaatc agatacagag
     1621 actcaggaaa acataaccga aaagcaacaa gtattaaata aaattaatgc ctgtatccat
     1681 aataccggag ccgaagtcat tcgccatatc ggctctaacg aggctgtata cgatattgat
     1741 cgtattaggc aagccttggg ggataaacaa ctgacagccg tggcgtattc gtatggaact
     1801 caaattgcag ccttatatgc agaacgtttt ccctacaacg taagatctat cgttcttgat
     1861 ggagtcgtcg atatcgatga cctggaggac aacttcacat ggcaactcaa acaggcacag
     1921 agttatcagg aaacgtttga tcgctttgca tcctggtgtg cgcgtacaaa aagttgcccg
     1981 ctttcttcag acagagataa ggcaataact cagttccatg agctattatc aaaattacat
     2041 cacaaacctt tattagacag taagggagaa aatatatctt cagatgaact catatcatta
     2101 acaacagacc ttctgctatg gcgttcatca tggccaaccc ttgcaactgc catacgccag
     2161 ttctctcagg ggattgtcag taatgaaatt gaaactgcgc tcagtgctcc gatagcctca
     2221 gaagagtcaa gcgatgcttc gggggtaatc ctctgtgtag atcaggggga tgagcaatta
     2281 acaccagaag agcgaaaatc ccgaaaagac gctcttgcga atgccttccc ggctattaac
     2341 tttgacaatg gacgttccga ttcacctgat ttttgtgaat tatggccaat acatagcgac
     2401 ctgaacaaaa ctcgcctgaa aaatactgtt ctgccctctg gtttactgtt tgtagcacac
     2461 aaatacgacc caacaacgcc ctggattaat gcccgtaaga tggcagagaa attttccagc
     2521 ccgttactaa caataaatgg tgatgggcat acattagctc tcaccggagt taatttatgt
     2581 gtagataaag cagttgtaca ttacctgatc actccacaaa aaatagaaaa tatatactgc
     2641 ccaggaaatt ctgaagcaga aatacaataa tttcaaaata ttccgctatt tgctcccaat
     2701 g
//
LOCUS       DAZGCS010000265         2692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGCS010000265 DAZGCS010000000
VERSION     DAZGCS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.270"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..205
                     /locus_tag="ACHK01_005162"
     CDS             <1..205
                     /locus_tag="ACHK01_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8099921.1"
                     /translation="NIIETNSNGWFPDTDGALITGLTFLAPKDATRVQGFFQHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
     gene            207..476
                     /locus_tag="ACHK01_005163"
     CDS             207..476
                     /locus_tag="ACHK01_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8099922.1"
                     /translation="MNILKKIMQRLCGCGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            588..1160
                     /locus_tag="ACHK01_005164"
     CDS             588..1160
                     /locus_tag="ACHK01_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO8099923.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGRGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPENGVFIKNSGNSAVCTLFDSVSFSHLVREGGVHPL
                     TRETITPSMIVKPEDCIFDSSKGNFIIKDG"
     gene            1378..2076
                     /gene="nleD"
                     /locus_tag="ACHK01_005165"
     CDS             1378..2076
                     /gene="nleD"
                     /locus_tag="ACHK01_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO8099924.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDADIESLVKTSSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRSESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
BASE COUNT          778 a          504 c          682 g          728 t
ORIGIN      
        1 aaacattatt gagacaaaca gcaatggctg gttcccggat acagatggcg cactcatcac
       61 cggactgacc tttcttgccc ccaaagatgc cacacgggtt cagggttttt ttcagcattt
      121 gcaggtcagg tttggtgacg ggccgtggca ggatgttaag gggctggatg aagtgggcag
      181 tgatacaggc agaacaggag aatgacatga acatattaaa aaaaattatg cagcgtctgt
      241 gcggttgcgg aaagcatgat gaccgtgaag acggggagtt acttacagca cagctgcgac
      301 tgggaccggc agacattctg gagtcagatg agaatggcat tatcccggag caggacaggg
      361 taatcacgca ggtggtgata ctggatgcgg ataaaaagca gatacagtgt gtggtaagac
      421 cgctgcaaat cctgcgtgct gacgggaggt gggaaaatat tggcggaatg aaatagccga
      481 cagcttcaca aaaaccggag tccggctccg gtttttgttg tcatgtcatg gtgatgtttg
      541 ttaataaaga cttgagggaa tatttatccg tatgaaggaa tatggtaatg cctggattaa
      601 tatcatatgt gtcatcggct tcattcgtga atgagatgat ggagctgcgt cagcaggtaa
      661 tggaggggca gattggtgga tttctcctgg gaggggagag ggttagagtt tcttatatgc
      721 cagatacagg ccgtttttta gcagaaagtg aagggcgggg acgggtttat gcagaattat
      781 tgaatattgc ttttaatgat ggagttaatg tgctcagaaa caggatatta agtgcgctcc
      841 ctggaatggg agggcgaaac tctttgcagg aaaaaatatc ggagtgtgcc tttactgtcg
      901 atattgaaaa acttcaatgt cctggtgatg cgcttcaatg tccaattaca ctggagcagc
      961 ctgaaaatgg tgttttcatt aagaattcag gaaattctgc ggtttgcacc ctgtttgatt
     1021 cggtttcttt ttctcatttg gtccgtgaag gtggggttca cccactgaca cgagagacaa
     1081 taactccatc aatgattgta aagccagaag attgtatatt tgacagttca aagggaaact
     1141 tcattataaa agatggttaa aatgttttac atgaagcaat attgattttc ggtagtttat
     1201 ataaaacacc acagacattc aaaatgtcag aggatgtcag tggtggatgt acctggagcg
     1261 gaagttgtgg aagtaagtta tgttatatcg caataaaccg aatacatctg tgatgttaca
     1321 ttggtctgag cttcagtgtg tggtgaatga aactcgtaac attttcagag gtccttgatg
     1381 cgccctacgt ccctcaactt ggcattacat cagtcatcaa cgtcgagttc aatgtcagat
     1441 gcagatatcg agtctcttgt aaaaacatcg agcgttcaat ggataaaaaa caatccgcaa
     1501 cttcgtttcc aggggactga tcataatata tatcaacaga ttgaagcagc gctcgataag
     1561 attggctcta cagagacagg gcgtgtactc ctgaatgcta ttgaatcaat atcccgactt
     1621 aaatcagaaa cagtggtaat acacctcaat tcttccagac taggagttat tgcacataga
     1681 gatatagatg ctgagaacca tcggggaact ggttccgatt ttcactgtaa tctgaatgca
     1741 gttgaatatc cctgtgggga ggggattagc gtggtggact ttcatgcgac tattgttttt
     1801 catgagctgc tccatgtgtt ccacaattta aatggggagc gcctgaaagt tgagagttcc
     1861 cgatcagaat cacaaaaata ctctccactt ttactcgaag aagccagaac tgttgggttg
     1921 ggggcttttt cagaggaggt gctttcagaa aataaattcc gcgaagagat tgggatgccc
     1981 cgtagaacat cctaccctca cgactcagct ctcattcatg atgacaatac agtgagtcag
     2041 ggattcctac aggtaagact gcatccattg ctttaggtgt gtttcttgtg gtgaatatat
     2101 attggtggga taaaactgag atacataagg tctcacaatg gcttgcaagg ctttgtgctt
     2161 ctctgtagag gcgacatgtt tttatccggt gcgccgtaaa accccgtcct tcagggcgtg
     2221 gaggatgtca aataataagg aatagtggat atgtttgcag caaagcccga gagcagtcaa
     2281 agaagtattt ctggattatt cagtcctagg tcaaatggtg aaaacgcaag taataaaccg
     2341 tcaagtggta tggcgaacag aatcaaacca gttgtaagcg attcacaaat cggtagatat
     2401 acaactgaca gtaatactct gaaggatgga ttgtgtagcc aagcccacat aggaactcaa
     2461 ccttatcggc caggtgctga ctcgccgcta actgtgagat cattcctaca aattacactt
     2521 tccaacaata attacaaatc atgagtctgc cctgtccctt ctgccaggaa gtgttacgca
     2581 acgatatctc accaatcttt cgcaccacta ttaatcaatg agttagaggt gattagtgac
     2641 ctgagacaga gcattagcgc aaggtgattt tttgtccgta agcgtaaacc ga
//
LOCUS       DAZGCS010000266         2664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGCS010000266 DAZGCS010000000
VERSION     DAZGCS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.271"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHK01_005166"
     CDS             complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHK01_005166"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO8099925.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHK01_005167"
     CDS             complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHK01_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO8099926.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          661 a          658 c          638 g          707 t
ORIGIN      
        1 atgttgcgtt tgtcatcagt ctcagcccgc ttttcagcgg gcttcattgt ttttaatgct
       61 tacagcactg ccacaatcgc ttcgcacagc ggagccatgt tatctggtgt catcccggcc
      121 acatttacgc gaccagaagc aaccgcatat acgccaaact cttcgcgcag acgcagcact
      181 tgttcttttg tcaggccact gaaggagaac atgccgttct gtttgatgat aaagctgaag
      241 tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca actgacgcat acgctgaata
      301 cgctggcgca tatcagtcag ctcttgttcc caaatcgcac gtaacgcatc gttgctcagg
      361 atggtggcaa caacagaagc gccgtgtgct ggtgggttag agtagttagc gcgaatcgcc
      421 gctttcattt ggctgaatgc gcgatcaacg gtttcactgt cggcagcaac cagagtacaa
      481 gcgccaacac gctcgttgta caggccaaag tttttagagt aggaactggc aacaatcagc
      541 tctttatgca tagccgcgaa agcgcgcagt ccttcagcat cttcttccag accacgggca
      601 aaaccctggt aagcgaagtc aaacagcggt aaccagcctt tctcaacgga gagttgtgcc
      661 agtgtctgcc attgttccag cgtagggtcg ataccggttg ggttatggca gcagccatgg
      721 aacagcacta cgtcgccagc ctgagcttca ttcaggctgt taatcagtgc atcgaagtca
      781 agagtgtgat tttccgcatc ataataagcg tattcacgaa cttccagacc tgcagagtta
      841 aagacgctct tatggttcgg ccagcttggg ttgctcaccc acactcgctt aacgctggta
      901 ttttttgcca ggaaatcggc agctacgcgt agtgcgccag tgccccccgg agtctgtgcc
      961 gtgcgagcac gtttgtcatt gatcagggcg ctacctttac caaacagcag ttcctgagtg
     1021 cagcgaccaa attcagggat gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg
     1081 agcagatact gttcagcctt tttcacgctg gtcagtaccg gggttttgcc cgtctcatct
     1141 ttatagacac caatcccgag gttaattttg ccgggacgtt catcggcacg aaacagatcg
     1201 gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc
     1261 cattatggtt acagaaggga agtccgctat cagggtaacg ggagatttac aaaattccaa
     1321 ctattactga tgaaaacgca ggctgttttt gcaagacgtg agattgctct ggaaggtata
     1381 aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac aaagaggtgt gctattagaa
     1441 ctggtaaacg atacccacag caacggtgtc gtctgaacct acgcccagtt tgttgtcaga
     1501 atcgatctgg ttgatgatgt agtcaacata ggtggacatg tttttgttga agtagtaggt
     1561 tgcgcccact tcaaagtagt tcaccagatc aacatcaccg ataccttcta cgtctttcgc
     1621 tttagatttg gtgtaagcga tggacggacg cagaccgaaa tcgaactggt attgcgcaac
     1681 taacagaacg tcttgcgttt tgttggcgaa gccgctggtg tttgtaaatt tattagtgat
     1741 cggcgtagcg ttacgggttt caccgtagtt cgctgccagg tagatgttgt tcgcgtcgta
     1801 cttcagacca gtagcccact gttcagcttt tttaccgttg ccaagaggtt gagcttcttg
     1861 caggttggta cggtcagctg caccataagc accaacgata ccaaagcctt cgtattcgta
     1921 gctgatagaa ccgccaacac cgtcgccgtt agaacggcgt gcagtgtcac gctcgttttt
     1981 acccaggtac tgaacagcga agttcaggcc atcaaccaga ccaaagaagt tggagttacg
     2041 ataggtagca acgccgccaa cacgaccaac gaagaagtca tcgctgtatg cagtatcacc
     2101 accaaattct ggcagcatat cggtgtaacc cagtgcatca taaaccacac cgtagttacg
     2161 gccgtaatcg aaagaaccaa cgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt
     2221 gttaccagtt tgagcgtcag cgccttcaga gttgttaccc tggaagttat attcccactg
     2281 accataaccg gtcagatcgg aattgatttg agtttcccct ttaaaaccaa gacgggcata
     2341 ggtcatgtcg ccattgccac cgtaactgtt ttcaccgtta cccttggaaa aataatgcag
     2401 accaacagct ttaccgtaca gatctacttt gttgccatct ttgttataga tttctgcagc
     2461 gtttgcagta cctgctacta acagagcagg gacgatcact gccagaatat tgcgcttcat
     2521 cattatttat taccctcatg gtttttttta tgacacctgc cactgccgtc aataagttct
     2581 gtcaataaaa atttacggaa ctattgatga gagtttggtg tctttatgtg tctgcaggca
     2641 tctttccatt caaactaacg tttc
//
LOCUS       DAZGCS010000267         2664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGCS010000267 DAZGCS010000000
VERSION     DAZGCS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.272"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            410..553
                     /locus_tag="ACHK01_005168"
     CDS             410..553
                     /locus_tag="ACHK01_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO8099927.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            566..775
                     /gene="cspE"
                     /locus_tag="ACHK01_005169"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             566..775
                     /gene="cspE"
                     /locus_tag="ACHK01_005169"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8099928.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            941..1750
                     /gene="rlmA"
                     /locus_tag="ACHK01_005170"
     CDS             941..1750
                     /gene="rlmA"
                     /locus_tag="ACHK01_005170"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO8099929.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1747..2313)
                     /gene="mntP"
                     /locus_tag="ACHK01_005171"
     CDS             complement(1747..2313)
                     /gene="mntP"
                     /locus_tag="ACHK01_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO8099930.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          721 a          615 c          627 g          701 t
ORIGIN      
        1 tcaatttttc ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat
       61 tgatagccgt taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
      121 agcaaaaaat gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac
      181 tgaacttatt gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag
      241 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
      301 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
      361 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
      421 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
      481 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
      541 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
      601 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagatg tgttcgtaca
      661 cttctccgct atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt
      721 cgaaattcag gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
      781 tccactgatc tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag
      841 gctttttact atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc
      901 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact
      961 ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
     1021 tgatatggcg aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga
     1081 tccgggcgac agcgcggaaa tgatacaagc acgccgcgca ttcttagatg ccggacatta
     1141 tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac
     1201 ggcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgtgtt
     1261 gcccgaaatc accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa
     1321 acgctatccg caggtcactc tttgtgtcgc ttccagccac cgtttgccgt tttccgatac
     1381 cagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt
     1441 agtgaagccc ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct
     1501 gaaggggctg atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg
     1561 ttttacatta cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc
     1621 cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac
     1681 actggcagca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
     1741 ttcttattaa ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagcaccag
     1801 cccgccgaga atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat
     1861 ccctaatgtc gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt
     1921 gacctgcagg aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc
     1981 ggtggttacc agtagccaga aaccgtgccg acggcgcggc tcttcatctt catcatctgc
     2041 gccacgaaaa ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat
     2101 ccagtggttc cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag
     2161 cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt
     2221 cggtttatgg agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc
     2281 aaacgcaaga agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata
     2341 aagcacatca tcgcccccag caaacatgca tacatgaaca acaagccgcg atggttttcg
     2401 cgttcatgca ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt
     2461 ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat
     2521 cggctactcc ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt
     2581 ggaatatatt atttcattgc tttattgata acgatttaca tttagattta tatatcaatg
     2641 aaacgttaaa ataaataaca aata
//
LOCUS       DAZGCS010000268         2664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGCS010000268 DAZGCS010000000
VERSION     DAZGCS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.273"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..608
                     /locus_tag="ACHK01_005172"
     CDS             <1..608
                     /locus_tag="ACHK01_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO8099931.1"
                     /translation="SPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQSN
                     FMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIGK
                     ETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGGDPITRFNTQ
                     TRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
     gene            1055..>2664
                     /gene="tir"
                     /locus_tag="ACHK01_005173"
     CDS             1055..>2664
                     /gene="tir"
                     /locus_tag="ACHK01_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO8099932.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSDQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPTTTDPDTAASTAEAATKDRLT
                     QEAFQDPDKQKVNIDENGNAIPSGELIDDVVAQIAEQAKAAGEQARQEAIESNSQAQK
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARIPEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANASPTPGPAR"
BASE COUNT          777 a          534 c          641 g          712 t
ORIGIN      
        1 ttagtccaat gacaatggta ggtagatcgt tagctcaggc ggctacacaa actcttagac
       61 ctgcggtcac cagagctgcg acacaagcgg gggtgggagc cagtggtatg aagttcgtgc
      121 ccatgcagtc gaactttatg attaatcatg gcaaactgac taaccagcta cttcaggccg
      181 tggccaaaca aacccgtagc ggtgatactc agcaatggtt caaacaggag caaattactt
      241 atatatccag aacagtaaac agagctattg atgactattg catgagtaat aattcggtaa
      301 ttggtaagga gaccaaaagc cacattttca aggctgttga aagcgcgata aagcagcctc
      361 tcgatatgaa ttgtgcacaa acgtcgattg gccatttctt acagtccaac aaatacttta
      421 ataaaaaagt tgatgagcag tgtggcaaag ggggggatcc aattacacgc tttaacaccc
      481 agaccaggat gatagaacaa gtctcacaag aaatctttga acagaaattt ggtggtttga
      541 aagttagtga tatcaaagct ctgactcaga gcacgattgc acagcatgta caggatactc
      601 ggttgtagga ataacctgtt atatatgcgt gttgggcata agttaagctt cctggtgtat
      661 agcgtagtct ttgctgatgt aagaaaaggc tatatacgga aacttgatat actataaact
      721 tcattattta ttatattgaa aaagttattt aattgatttg ttttgtaatt ttaatgcttc
      781 aattggataa gcataaaata tagttttatt tatagtatgt tttattaaag ttttacgtgg
      841 catttatttt tatatcgcat gattattttc atttaattac actgatgtgt atttttctgc
      901 attattttct tgcatcaaaa atcagactgt gatttatttg gtcgatgtta tgttcagccg
      961 tatatcgact acgtactgta taccattaca ttattatgta atagcaatat aaaggattat
     1021 tatctattta cataaaataa aaaggagaca ttttatgcct attggtaatc ttggccacaa
     1081 tcccaatgtg agagctttaa ttccacctgc accgccatta ccttcacaaa ccgacggtgc
     1141 aggaggtgcc cgtaatcagc tcattaactc aaatggcccg atggggtctc gtttgctatt
     1201 tacgcctata aggaattctg ttgctgatgc tgctgattct cgtgccagtg atattcccgg
     1261 acttcctaca aatccactgc gctttgctgc gtccgaggta tctttgcatg gtgcgcttga
     1321 agttcttcat gataaagggg ggcttgatac tcttaactct gctattggat cttcgttatt
     1381 ccgtgttgaa actcgggatg atggcagcca tgttgctatc gggcaaaaaa atggcctcga
     1441 gaccactgtt gttttaagtg atcaagagtt ttctagctta cagtcccttg atcctgaagg
     1501 taaaaacaaa tttgtattta ctggaggccg cggtggcgca gggcatgcta tggtcacggt
     1561 tgcttcagat atcgccgaag cccgtcagag gataatagat aaattagaac caaaggatac
     1621 aaaggagacg aaggagccag gggatccaaa tagtggcgag ggaaaaatca ttgaaattca
     1681 tacctcaacc tcaacttcta gcctccgtgc agatcctaaa ctttggttgt cattggggac
     1741 tattgctgca ggtctgatag ggatggctgc gacgggtatt gcacaggctg ttgcgttgac
     1801 tccagagccg gatgatccaa ctactaccga tcctgatact gcagcaagca cagctgaagc
     1861 tgcgacaaaa gatcggttaa cgcaggaggc attccaggat ccagataaac aaaaagttaa
     1921 tatcgatgag aacggaaatg caattccgtc aggggaatta atagatgatg ttgttgcgca
     1981 aatagcagaa caagctaaag cggcggggga acaggccaga caggaagcta ttgaaagtaa
     2041 ttctcaggcg cagaaaaaat atgatgagca gcatgctaaa cgcgaacagg aaatgtctct
     2101 ttcatcgggg gttggctacg gtattagtgg ggcgctgatt cttggcgggg gaattggtgc
     2161 cggtgttact gctgctcttc atcggaaaaa ccaaccggca gaacaaacaa tcactacacg
     2221 tacagtagtc gataatcagc ctacgaataa cgcatctgca cagggcaata ctgacacaag
     2281 tgggccagaa gagtccccgg cgagcaggcg caattcgaat gccagccttg catcgaacgg
     2341 gtctgatacc tccagtacgg gcacggtaga gaatccgtat gctgacgttg gaatgcccag
     2401 aaatgattca ctggctcgta ttccagagga acctatttat gatgaggtcg ctgcagatcc
     2461 taattatagc gtcattcaac atttttcagg gaacagccca gttaccggaa ggttagtggg
     2521 aaccccaggg caaggcatcc aaagtactta tgcgcttctg gcaagcagcg gcggattgcg
     2581 tttaggtatg ggaggattaa cggggggggg cgagagcgca gtaagtactg ccaatgcctc
     2641 accaacgccg ggacccgcac gttt
//
LOCUS       DAZGCS010000269         2626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGCS010000269 DAZGCS010000000
VERSION     DAZGCS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.274"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..339)
                     /locus_tag="ACHK01_005174"
     CDS             complement(<1..339)
                     /locus_tag="ACHK01_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099933.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTN"
     gene            complement(378..1607)
                     /locus_tag="ACHK01_005175"
     CDS             complement(378..1607)
                     /locus_tag="ACHK01_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO8099934.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDSDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1895..2560)
                     /locus_tag="ACHK01_005176"
     CDS             complement(1895..2560)
                     /locus_tag="ACHK01_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099935.1"
                     /translation="MLQRDPHGFRKDVKEFITMIKSFQVDIRTENNLTSAYKHVLFLE
                     FIHNSHPEISHKTFIKSIIYDVLSSITAILHKRERYLHLNFRSMIEHLARISLQKVDN
                     GGDFDITIRTLDFENLKSTNTDENWAYMHSQYKQACGWLHSSSKVKLNITASFPDLLK
                     SDSKSNAAKLSTHLQKMTTEMLKIFFNYYVDEIQTSFYRTRGEMRYVLGNHNFEYFLS
                     KNP"
BASE COUNT          719 a          515 c          519 g          873 t
ORIGIN      
        1 gttagttaat aactttaaat ttaatgcttc aggattttca ctgagaaagt gaaaggcata
       61 aactaagtat acagctaaag tagccgttcc aattgttggt ccaatatcgc cttgatcatg
      121 aggttcaagg tttacattga acttgaagat tagccattca aatccccaag taaaaaaaac
      181 aactaatatc atggctgcga ataatttcgc gatgttatct gctgatggat tcatcagaac
      241 agattgtttc tgaaaaaatt cacaaagtga gagtccgaaa aaaataaaaa aaagtggtct
      301 ggaactaaat ttttcaaaaa ctttgaatag ggtttccatt ttctttacct tcaggattat
      361 attagctatt ctgatactta ggctaccaga gcatttgatc tttgagcttg aacgtaatca
      421 ctccaccatt gcatcaaact ctgtcgctct atcagatatt ctgcacgatt gtatgctgcg
      481 ataatttcat cttttttcga gtgggcaagc gctgcctcaa gaacttcagc tctgaattta
      541 ccagactcct ctgccgctgt tcgtgcaata gaacgcatac cgtgagctac aagctcgcct
      601 ccgaacccca ttcggatgat agctgcgttg gctgtttgtt catgcatatg attaagaggc
      661 gcttttatgc tggggaaaac ccattctcta tgcccactta ttgatttcat taattcaagg
      721 atgcgcaaag cttctttact caaaggaact ttgtgaagct ttttcatttt catgaaatca
      781 gcaggaatgt tccaaatgct gttggttgta tcaatatcag accaccttgc gcgaacggct
      841 tcacccggac gaacccatgt caacaattgc cattcaatta gcatacgtgt ttccaaccgg
      901 attgacgcat tcgtcaaaga ttccataaac cttggcaatt cgcttggggg aagggcaggc
      961 atattttgct tttttggttt actgaatctt tgaccaaggt tgtcagccgg gttgaactca
     1021 ataagttctt cagtagctgc ccaccggaag atttcattca gacgtgaaat gatacggcgt
     1081 agagtttcca atacgcctcg ttgctcaata ggatcaaggt gttgttttaa gagcttaggt
     1141 cggatctcat tgatagggac attacccaga ccgggaaaga catttctctc taagctgcgc
     1201 cagatgtctg ctgcatggtc ttgtgagata cctgatgtct ttaccttctc atctaaccat
     1261 ttccgcgcta cggcttggag agtgtgctca gtagcactct ttaatgcctt cgccttatcg
     1321 ttgttatgga tttggggatc aacaccattt gccagaaagg agagatattc atcacgtaag
     1381 gctctggctc ttgcaagggt aaggtgagga tatgtcccaa ggctcatttt ggttcttttc
     1441 ttgctcactg gtactgcata cctgaaatac caatttttct tgcctccttt cgccaaagga
     1501 gcgattcgta gaatcagacc atcactgtca aacaagttga tttctttatc ggctggcttg
     1561 gtgcttttga tttcagtgtc agtgagcttc ttagcgattt ttgccatttt gggaccctcg
     1621 gtttttggac ccttcttagt gggtcccatt cagggtgcca taactcgtag ttctcagcaa
     1681 ttctcactgg acgacaatag acgtaaaaaa gcccgcagag cttgtgctgt gtgggcttag
     1741 tagacttcat tgaacttcaa acaactaaaa agtggtggag ctggcgggag ttgaaccagc
     1801 gtccatttgt ttttaattta ttgattttta ttgttttatt ttatttctcg catgcttcgt
     1861 gtacttcacg tatccttttt aaggaaatat atatttatgg attcttgctt aaaaaatatt
     1921 cgaagttatg gttgccaagc acatacctca tttctccccg tgtgcggtaa aaagaagttt
     1981 gaatctcatc aacatagtag ttaaaaaaaa tctttaacat ttcggtagtc atcttttgca
     2041 aatgggttga cagtttagct gcattacttt ttgaatcaga ctttaataaa tctggaaatg
     2101 atgctgtgat atttaattta actttagatg atgagtgtaa ccagccacat gcttgtttat
     2161 actgtgaatg catgtacgcc cagttttcat cagtgttggt agatttaaga ttctcgaaat
     2221 caagagttct aattgtaata tcgaaatccc ccccgttatc aactttttgt aaggatatac
     2281 gtgcaagatg ttcaatcatt gagcgaaaat taagatgaag atatcgctct cgtttatgaa
     2341 gtatcgctgt aattgaactt aaaacatcat aaataatact ttttataaag gttttgtgac
     2401 ttatttctgg atgtgagtta tggataaact ccagaaaaag tacatgttta taagccgatg
     2461 taagattgtt ttcggtgcga atatcgacct gaaatgattt tatcattgtg ataaattctt
     2521 tcacgtcttt tctaaatcca tgtgggtctc tttgaagcat tattttctat ccttcttcaa
     2581 aagagcatta atccacgtac ctacatctcg atttgcatta cgtttg
//
LOCUS       DAZGCS010000270         2615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGCS010000270 DAZGCS010000000
VERSION     DAZGCS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.275"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            391..1368
                     /locus_tag="ACHK01_005177"
                     /pseudo
     CDS             391..1368
                     /locus_tag="ACHK01_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1887..>2615
                     /locus_tag="ACHK01_005178"
     CDS             1887..>2615
                     /locus_tag="ACHK01_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFO8099936.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHKQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNSLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYAN"
BASE COUNT          859 a          543 c          556 g          657 t
ORIGIN      
        1 taaaattgag tagagaagct cagccacttg tgtagggggg ctaaaattaa ttctatttca
       61 ttatatgaga acaccaataa gaataataat atttgatcac aactgaagat gctttttaca
      121 atgaagaatt attgttaaaa aacgctttgt tgaatcaacc gaactgttgc tgagttgcag
      181 gatcacatca cgtattttcc tgaaaacgca ggctgcttag tagcaaagca gaagttcaaa
      241 atcaccaact aaatagctgc gccgaatagt agatcaatga aagggaactc agcccggatt
      301 gtgcgatctg atcaatcgcc aaaaatcccc ccctaaccgg actgagcgat gccgatcata
      361 gcaccaattc cccgcgacga atgacgcctg atgcaaaaag caatctataa aactcacgat
      421 aaaaattatg cccgcaggct gaccgccatg ctgatgctgc accggagcgc tcgcgtcagt
      481 gacgttgcca gaacgctctg ctgcgcccgt tcctccgtca ggcgctggat taactggttc
      541 acgctgtcga gtgttgaagg tctgaaatca ttacccgcag gacgttcccg ccgctggccg
      601 tttgagcata tctgcacatt gtttcgtgaa ctgataaaac actctcccgg atattttggc
      661 tatcagcgtt cacgctggag tacagaactg ctggcaataa aaatcaacga gataaccgag
      721 tgccagttac atgccggaac cgttcgccac tggttgccat tagtcgggct tgtgtggcgc
      781 agggctgcgc caaccctgcg tacccgtaac ccgcataaag cagcaattca caaagcactg
      841 gacgaatgta gtgcggagca tccggtcttt tatgaagatg aagtgtatat ctatcttaat
      901 cccaaaaccg gtgcggactg gcaacttcgc agacagcaaa aacatgtggt aacaccgggg
      961 cagaatgaaa aatactatct ggctggtgct ttacatagtg gaacgggcaa agtcagctat
     1021 gtgggtggca acagcaaaag ctcagcctta tttatcagtc tgttgaaaca tctgaaattg
     1081 acataccgac gggataaacc tatcacgctc atagtggaca actacatcat ccataaaagt
     1141 cgggaaacac agcgctggct gaaagaaaat ccgaagttca gagtgattta tcagccagtt
     1201 tactcgccgt gggtgaatca tgttgagcgg ttatagcagg cgcttcacga tacgataacg
     1261 cgcaatcatc agtgccgttc aatgtggtaa ctgcttaaaa aggttcatta ttttatggaa
     1321 accgtgagcc cattccccgg tggtaaatat gggctgttaa aagtgtagcg gtattcggcg
     1381 cagctattta ggatggtcag gtcgcagaag ttctgtgcca ctacagtgcg tgtactaaac
     1441 aaaatgacga gggcacgaat gtcataatac gtgcgtatta cctgcagtag ctaccaactg
     1501 agggggctta tcccaaacct gatttattca acaaagctgg acttatcctt actctgaatt
     1561 attttccaga catatgcatc atgtactggc tcgcttggtt caggaaatac aggagcaaat
     1621 ctacagctat tagatttatt cctatttgta tgcaaagtcg cattgcataa tgcggagcta
     1681 tgttacagat acgcctgacg tgcgtagtaa aattataaag caacatatat atacaaggag
     1741 aaaccgagaa aaataaatga gtcgtatgtt gggtagcatc gagataaata ttttttttat
     1801 cacattagca ccttggttaa gggtgttaat aataatattc tctcacttat taaaacataa
     1861 ataatttttt aagcggaaat gtaattatga gactgccaga gaaagttctt tttcctcctg
     1921 tcactagtgg cctgtcaggg caggaaaaac aaaaaaaacc gaagagcatt accggatttc
     1981 aggaaaatta tcaacgcaat atcaggccaa tcaaaacagc atcagaagcc cgactacgct
     2041 tctttgataa aatggtttcg aaagaaaact ctctggaaga tgttgtttct ttaggtgaaa
     2101 tgattcagaa ggaaatttat gggcataaac aaagaacatt ttcaccagtt catcatacag
     2161 gtaactggaa atcatcattg ttacacaacg cgctccttgg tctggcaaat gtttataatg
     2221 gcttacggga aacagaatac cctaacactt tcaacagaga tggtataaaa agtactaact
     2281 cttttagaga taactcattg acaaaaacaa gaactcccag agataatttt gaggaaggaa
     2341 taaaacatcc tgaacatgca acaataccat atgacaacga caatgaaagt aataaattgc
     2401 taaaagcagg aaagatagct ggtaacaata acgagctgtt gatggaaata aaaaaggaat
     2461 cccaaagcga ccatcaaatc cccctgtcag ataagttcct gaaaaggaaa aaacgatctc
     2521 ctgtagctga agataaagtt caaaactcgt taacaccaga aaattttgtt cagaaaattt
     2581 cacttagtga tgagcttaaa acaaaatatg caaat
//
LOCUS       DAZGCS010000271         2548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGCS010000271 DAZGCS010000000
VERSION     DAZGCS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.276"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..383)
                     /locus_tag="ACHK01_005179"
     CDS             complement(<1..383)
                     /locus_tag="ACHK01_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8099937.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
     gene            complement(380..958)
                     /locus_tag="ACHK01_005180"
     CDS             complement(380..958)
                     /locus_tag="ACHK01_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099938.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(970..1323)
                     /locus_tag="ACHK01_005181"
     CDS             complement(970..1323)
                     /locus_tag="ACHK01_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8099939.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1335..1730)
                     /locus_tag="ACHK01_005182"
     CDS             complement(1335..1730)
                     /locus_tag="ACHK01_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8099940.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1772..>2548)
                     /locus_tag="ACHK01_005183"
     CDS             complement(1772..>2548)
                     /locus_tag="ACHK01_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein E"
                     /protein_id="HFO8099941.1"
                     /translation="VNPQMTLRRLPDEDPQNLADPAYRRRRIIMQNMRDEELAIAQVE
                     EMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSGGTEWSKRDKSTYDPTDDIE
                     AYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGSHSELETAVKDLGKAVSYKG
                     MYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINAS
                     ARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
BASE COUNT          532 a          800 c          640 g          576 t
ORIGIN      
        1 gtaatggaat aagtcagatc tgcagaactc cataacgcca tatcgtcatc acgacgatac
       61 tcatagccct gcgtaaccat cgtggtaatc agtcctgcca gtgccgggat atcgctcatc
      121 accggataaa tccgggactc catccacgca tccagctctg aatccggcac ctgagcaggc
      181 aggaaaactt caatatgcag ctccgcctgc caggtatcgc tgtccagctc ttcgcccgtg
      241 tattcagcgc cggtgagata aacggcaact gccggaaaat ccgcctcatc aaaaacagcg
      301 gggcgaccat caaaaagcgt cgccccggtg tcatgcttct ccagtgcatc cagtacggct
      361 gcacggagtt cagtatgttt catcgcttta ttaccattct cagttgatgc tgcagcgcat
      421 agcccagctc tttcggaaga cgttcacgcc gtatccgttc aatattctgt ttaaacgccg
      481 tggtaagcgg caccgccatc gggattttca ccacatcaat ggggtaacgg tttttcccgg
      541 ccacacgctg catgacatgc caccgcccat ttttcagttg ctgaataaac gcgccgggaa
      601 tacgacggtt acccaccaca agcacgctgc cgccaccttt cagggatgaa cgctgcccct
      661 ttttacgacg cctgcgtcgg gacaggacaa cccgcgcatt ccccagcttg attacgggca
      721 aatccccccg gttaaccctg attctggcct gcggattttt aaccgtggcc cttttcagcc
      781 tggccctttc ctttaccagt ttccggcgta cctttgtctc acgggcaacc tgtgacgcag
      841 actgcgatat cgcggatgaa gcaacgcggt taatggccat tgctgacgca ccgggcaccg
      901 ccgttttgct gatacggctg aggttttcaa cggcctgctc aagacctttt atggccatac
      961 atcccccttt cagcggcgac ggttaacggc aggcggcacg ccacgcccaa gccagagatg
     1021 acagcttccg ccatcatccg gcgaaatccg gtctatccag aagttttcct caccgatggt
     1081 cagcgtgtct ccacgccgca gttgccgcac atcatcagtc cggacaaaca gggacgggct
     1141 ggagccttca acgcgcacgc cctgtccggc atagctgata ttttcagggt catcaaaaac
     1201 accacgtatt actgcgccgg actgctcacc ggatgtcatg gtggctgacg ttcccatgta
     1261 cccgcgtatc gtttcatcgg cgcgggcaat ggcagcatcg aacaggttat cgaaatcagc
     1321 cacagcgcct cccgttattg cattctggcc aggccacgtt ctgtcatttc ggctgccaca
     1381 ccggcagaga cacggaacgc cgttcccggc agcacaaatg ccacaggttc atcccgcgtg
     1441 gcgtgaagtg catcggtatg cagcgtcacc agtgccacaa ccgtgaccag agcagccgta
     1501 tcagtcacgg tatccgtctg tgctgatacc acctcatttt catgtccggt cagcgcattt
     1561 tccgggctga cagacgtgtc ctgaccggct gcgtcatccg tgtcatcaag ctcctcttcc
     1621 agctctgcca cacggagcgc cagttcttct ttcgtccccg tcaggctgac atcacggttc
     1681 agttgttcac ccagcgagcg gagacgggca atcagttcat ctttcgtcat ggactcatcc
     1741 acagagaaac aatggccccg aagggccata attacgccag ttgtacggac acgaactcat
     1801 cagggtcagc cagcagcatc agcggtgctg actgaatcat ggtgaactct cgcgccggat
     1861 cgccggtagt cacccagttt ttcgggtaac gggcagaggc gttaatgcct tcgcgctgtg
     1921 cgtccgcatc ctgaatacag ccataggtac gcagaccgcg tgcctgagtg ttccccagca
     1981 ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac tgtccggaat
     2041 acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg cccaggtctt
     2101 ttaccgctgt ctccagctcg gaatgagagc cgcgacgggt atccagctta tccctgacgg
     2161 ctttgaagga acggaacagc gcccatcctt tcggatcaaa cacgatgata ttcaccacgc
     2221 cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg gacttgtcac
     2281 gcttgctcca ctccgtgcca ccggactgtg tgatgttgtt tgccgcactg cggcccatat
     2341 ccacctcaac cggatcaaag gcttcaccgg tcatggtgta tttgccctta agcacggcag
     2401 aaactgcctg catctcttcg acctgggcaa tggccagctc ttcgtcacgc atgttctgca
     2461 tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct tcatccggca
     2521 ggcgacgcag ggtcatctgc ggattcac
//
LOCUS       DAZGCS010000272         2540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGCS010000272 DAZGCS010000000
VERSION     DAZGCS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.277"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2540
                     /locus_tag="ACHK01_005184"
     CDS             <1..>2540
                     /locus_tag="ACHK01_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8099942.1"
                     /translation="GVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILGNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVADNEGVGFRYSFSAL
                     KDRHTAVEVSYVDPHNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPLSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTNGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEKRITNTAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFAV"
BASE COUNT          580 a          681 c          793 g          486 t
ORIGIN      
        1 ggggtgcagt cactgttgga gaccacctca aagggcgacc gtaatccctc ttctgtccga
       61 ctgctgattc agttgcagcg taacggtaac tgggtgacgg aaaaggatgt caccattaac
      121 ggcaagacca cctcgcagtt tctggcgtcg gtgattctgg gtaatctgcc gccccgtcct
      181 tttaacatcc ggatggtccg ggagacggcg gacagcacct cggaccagct gcagaataag
      241 acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg
      301 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagatgac ggtgaactac
      361 catatccgcg gtcgcatcat tcaggtaccg tcaaactatg acccggaaaa acgcacttac
      421 agcggtatct gggacggcag cctgaaaccg gcatacagca ataacccggc ctggtgcctg
      481 tgggacatgc tgacccaccc gcgctacgga atgggaaaac gtctgggggc ggcggatgtg
      541 gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
      601 gggggcacag agccgcggat gacctttaat gcgtacctgt cacaacagcg taaggcgtgg
      661 gacgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg
      721 ctgacgtttg tgcaggaccg tccgtcagat gtggtgtggc cctacacaaa cagtgatgtg
      781 gtggctgaca atgaaggtgt gggattccgc tacagtttca gcgccctgaa ggaccgtcat
      841 actgcggtgg aggtcagtta cgtcgacccg cataacggct ggcagacctc cacggaactg
      901 gtggaagacc cggaagccat actgcgctac gggcgcaacc tgctgaagat ggatgcgttc
      961 ggttgcacca gtcgcggtca ggcccaccgt gccgggctgt gggtgataaa gaccggactg
     1021 ctggaaacgc agacggtgga tttcacgctc gggtcacagg ggctgcgtca cacacccggt
     1081 gacattattg aaatctgtga taacgactat gccgggacca tgaccggcgg acgtatcctg
     1141 tccatcgatg ccgccagccg caccctgaca ctggaccgtg aggtgaccct gccggagaca
     1201 ggtgccgcca cggtgaacct gattaacggc agcggtaagc cggtgagcgt ggccatcact
     1261 gcacaccccg cgccggaccg gatacaggtc agcaccctgc ctgatggtgt ggagacatac
     1321 ggtgtatggg gactctccct gccgtcactg cgtcgtcgcc tgttccgctg tgtctccatc
     1381 cgggaaaaca cggacggcac ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa
     1441 gccattgtgg ataacggggc cagctttgag ccactgtccg gttcgctgaa cagcgtcatc
     1501 ccgccggctg tgcagcacct gacggtggag gtgagcgcgg ctgacggtca gtatctggca
     1561 caggcgaaat gggacacgcc gcgggtggtg aagggtgtgc gcttcagtct gcgcctgacc
     1621 aacggaagcg gagaagacag ccgtctggtg accaccgcca tcactgcgga tacagagcat
     1681 cgtttcagtg gtctgccgct cggggaatac accctgacag tcagggcaat taacagttat
     1741 ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc aaaacccgcc
     1801 accattgaac tgacgccggg gtattttcag ataacggcgg tcccgcgtct tgcggtgtat
     1861 gacccgacgg tacagtttga attctggttc tccgaaaaac gcatcacgaa cacggcacag
     1921 gtggaaacct ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc cggcccgcac
     1981 attaagcccg ggaaggattt ctggttttac gtgcgcagcg tcaacctggt ggggaaatct
     2041 gcgtttgtgg aagtcagcgg gcagcccagc aatgatggtg aagggtatct ggaatttttc
     2101 cgggaaaaaa taggaaaact gcatctggct caggggctgt gggagctgat agacaacagc
     2161 cagcttgcgg atgagatggc ggagatgaag accaccatca ccgaaacccg caatgaaatc
     2221 acacagacgg tcagtaaaac gctggaagac cagagcgcca ccattcagca gatacagcgc
     2281 gtgcagaagg acacaaatga tgacctggct gcgctgtaca tgctgaaggt tcaaaaaacg
     2341 aaagacggca ttccctatgt ggccgggatt ggtgcaggga ttgaggatac tgatggccag
     2401 ccactgagca acatactgct gctggctgac cgtatcgcga tgataaatcc ggagaacggc
     2461 aacagcacgc cgttatttgt ggcgcagggg aatcagttgt tcatgaacga tgtgttcctg
     2521 aagcggctgt ttgcggtgag
//
LOCUS       DAZGCS010000273         2453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGCS010000273 DAZGCS010000000
VERSION     DAZGCS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.278"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..287)
                     /locus_tag="ACHK01_005185"
     CDS             complement(90..287)
                     /locus_tag="ACHK01_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8099943.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(574..1392)
                     /locus_tag="ACHK01_005186"
     CDS             complement(574..1392)
                     /locus_tag="ACHK01_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO8099944.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1544..1915)
                     /locus_tag="ACHK01_005187"
     CDS             complement(1544..1915)
                     /locus_tag="ACHK01_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8099945.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1905..2276)
                     /locus_tag="ACHK01_005188"
     CDS             complement(1905..2276)
                     /locus_tag="ACHK01_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8099946.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2289..>2453)
                     /locus_tag="ACHK01_005189"
     CDS             complement(2289..>2453)
                     /locus_tag="ACHK01_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8099947.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          817 a          623 c          423 g          590 t
ORIGIN      
        1 aaggtaaatt atcatcaaca cataagcaaa ggactgacag gtgccgctaa cacccaccag
       61 ccgcccattt accacaaata aaaaagcctt caggactgaa ggcgtctgta acaaccgcac
      121 tgatagtctg ccagacccgc cataacaagc tgggtcagta ttaactggca gcgttcgcgt
      181 gaaaggtaag tattctgcgc aatctccccg actgtcgccg gttcggtgac gcttaattca
      241 tcaaacacaa ctctggcggt ttctgtcata tcctgctgtt tcagcatgtc tttttaccct
      301 ttccggttaa cgtgacacac caataactct tgccgaaaaa gccagcaagc tgaaagacct
      361 gtattaataa ctaccagcac atttaacgca ctgcgctact ttgcgggcac aaaaaacccg
      421 ctcagaggcg gggtcaagct atgcggcgaa ataaccactc ttaacagcat acctgatttt
      481 ttacgtacgt aaatgctttg ccgtgcatat ttttcatgca aatgtcacgt cctgctattt
      541 ttcagtctta taaatttaaa accatagaaa aaatcaatta tgttttaaaa atggataggt
      601 aaagaataac aagtgacaca gattcaaacc aaaatggaaa agggtggcaa cccacaaacg
      661 cccactccac atccatgaca atccatacac aacaccagat aacgtggcaa ataatacaag
      721 taaagcacca cctgaatagt gataaaaacc aaacaacaaa gccgccacaa ttaatgcaac
      781 caatggagac gttacttctg atagccgtga ttgaatatac cctctaaata atgattcctc
      841 tgccagagac acaaaaaaca aattagccaa tataaactct ggcaaccact caggaaaatg
      901 aatctctggc tttaatccac caaaaaaaac agccaaaaac aggataagag gaacagagag
      961 cgacagagcc ccccacttcc acaaagacac ttctgatttt acttcttttt taaacaaaga
     1021 agatgtacac aggactaaca aaaatggcac cagcgcttta tcaaaattaa aatacattgt
     1081 atagggagta ctctgagggc caacagtaac agaatttagc actacaggat tgtgaaaccc
     1141 tggccataga tggaaagata atgctatggc tgataaaact atgccaactt catatataga
     1201 tttagcccag gcgttatatt tccagttgaa cttcaaaata ataaaaaaaa cgattgtagc
     1261 aacaaaaaat aacaccgacc aatcaataat atcattaagc acagccagga caacagacac
     1321 cgtcaacaat gaaaaagcca ctaccttatg ccaactgaga gttgacagtg acagtactaa
     1381 cacaatccac ataagcactc cttttattta atgatgaaga ttgattatca atattttcaa
     1441 ttcaccaggc aacattttat ctaccttcca caatacatga ccatcaaaaa ataaacatgt
     1501 taattataaa cacagaaaac ataaccctca tcactatata tccctaccgc atatccatat
     1561 ctaaccggac attcagagcc ataagcattc cttcaataat accttccgct ttataaagcc
     1621 ttttaccaat aagcccatca gaacatctat gcttacgtgc aagagccata aatgtcattc
     1681 cacctacgta ataatccacc aacaaatcgt gcaaatactg attattcttg tttaatcgtg
     1741 ccatacaacc acaaattatc atggcgtcat catcagaaca ctgaggacgt gatttcactt
     1801 tagtcgggat taatccttta aaaccagcag ctatcgaggg ccatgttaca tcttcatgat
     1861 tatttgctgc ccatgccccc catcgctcaa gaaccatctg gatatcacgc gccatcgtta
     1921 ccacctctga tttcgtaaat cttcacgccc agccgtccac ctggcacagg ctgaccgcgc
     1981 acaatattga tttcatcaaa ctgctcgtca tcaatgagca cttccgcatg cgtcagcgca
     2041 tccagcggtg ctttcagaat gttgtccagg tcacggcggc gcttatccgg tggttctgca
     2101 ataattttta ttgccagccg tccggacagg cttaatttca gccgctgctg gcgaacaata
     2161 agcgccacag cccggcgata acgctcaccg gcttttgata caaaatatgt gctgccacga
     2221 cgtcgccagt aggtgttcac cgtcggcggg taaggcaaaa caaactctat acgcatcagt
     2281 aacctctttt acccgagcac gccggttgca aaagcgtgat caagaaaacg aaaaattaaa
     2341 tcaacctggg aaccatgctt ttcttcgaac gccagcggat ccgcatgaag ctcgttgtga
     2401 tgctcccgac acagcggtag cgtgaaaata tcgtgggatt ttgttcccat tcc
//
LOCUS       DAZGCS010000274         2408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGCS010000274 DAZGCS010000000
VERSION     DAZGCS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.279"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..326)
                     /locus_tag="ACHK01_005190"
     CDS             complement(<1..326)
                     /locus_tag="ACHK01_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8099948.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRV"
     gene            complement(326..655)
                     /locus_tag="ACHK01_005191"
     CDS             complement(326..655)
                     /locus_tag="ACHK01_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099949.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(652..>2408)
                     /locus_tag="ACHK01_005192"
     CDS             complement(652..>2408)
                     /locus_tag="ACHK01_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8099950.1"
                     /translation="ATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPE
                     SSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAAT
                     LQSDMEKAGELATRDRAERDASQLKYTGEAQKAYERLLTPLEKYTNRQEELNKALKDG
                     KILQADYNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDLTHAALLALETELRTLEK
                     HSGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHEQETLEYKRQLADL
                     GDKVEYQKRLNELANQAVRFEQQQSAKQAAISAKARGLTDRQAQRESESQRLRDVYGD
                     NPDALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDG
                     IAQNMAAMLTGSEQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASAS
                     TGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRG
                     YAEGGYVGGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQ
                     DEIQAQMRDGGVFSGGRR"
BASE COUNT          438 a          854 c          563 g          553 t
ORIGIN      
        1 tacacccggc ggcggaccac cgtggccccc accaggctct gcaggtcctc cgccattccg
       61 gtgaccagac cgaacagatt ggacaccgtc agcgacgggc gggcactgct gcccttcccg
      121 ttcatctcaa agccactgcc gtcaatcggg tatgcctgat attgccgccc ctgccaggta
      181 accgcctccc ctttttcatt cagctcattg cagaaaaaat accgctcacc accctgtacc
      241 gtcaggtcga tttcccagag taccacccgc ggtgactgct ctgatttaac cgactcgttc
      301 agactttctt cgtgaatatc ctgcatcagt tcaccacctg cttaaactcc gcgctgaatt
      361 caacccgcag catgcgaacc cgtgatgacc aggcggcaca ggtcaccttt atctgccgcc
      421 aggcataagg cggtgtccac agaaacgcct tccagccacc gtgctctgcc aggaatgcct
      481 ccagatgtcg ggcctcctcc cgggtcacgg aaagcgtcac acggtatgtt ttcaggtcag
      541 cattcagccc cgccgccata cgctgcgaat acccgtcacc aaaacgcact tcacgcaccg
      601 atggctgcga gttcacctcc atatccggct tcactttcca gcgaaatgtt ttcatcgcct
      661 gcctccggaa aagacgccgc catcacgcat ctgcgcctga atctcatcct gcgccccctt
      721 gcgggccatg tcatacaccg ccttcatcag ctgcggcccc gcctgtccgt tggtgccgtc
      781 gttctgaatc accacgtgat tgttctgatt aaaactaatg ccttccgccc gccgcatctg
      841 cgccggactt ccggcaccgc ccacataacc accttccgca tacccgcgca tcagacgata
      901 caggttgccg acgccaatcc ggctggttgc ctccttcgtg aagacaaact ccccgcggtg
      961 gacaataccg gcaggttcgt atttgccacc ggttcccgta aatcctccgg tcgcgaaatg
     1021 gaagttcgcc gccgcagcct gaatggccgt ccccgtggag gcagacgccc caccaccgaa
     1081 agcaccaccc atggcgctgc caatactccc gacaatcccc accatggcct gcttcagaaa
     1141 aatctctgtc agcatggaga gcacagaacg ggtgaaacca cgccagctct gttcgctgcc
     1201 ggtcagcatc gctgccatat tctgtgcaat accgtcaaag gtctgcgtgg ccacgctttt
     1261 aacctgcgaa aaactgtccg tcgcactttc tgcccactcg ccccagccgg acttcagacc
     1321 ggccatccag cttccacgaa gctgctcctc cgcagaccag gtgttcttca gtgcagatgt
     1381 ggccttcgcc agcgcatccg gattatcacc gtacacgtca cgaaggcgct gcgattccga
     1441 ctcccgctgt gcctgacggt ccgtcagccc ccgggctttt gcgctgattg cagcctgctt
     1501 cgcactctgc tgctgttcaa accgcacagc ctgatttgcc agctcattca gccgtttctg
     1561 gtattcaacc ttgtcaccca ggtcagccag ctggcgtttg tactccagcg tctcttgctc
     1621 atgggccagc agggatttct cctgtgcaga cagctggcga cgttgcgccg cctcctccag
     1681 taccgcgaac tgactctccg ccttccacaa atcccggcgc tgctggctga ttttctcatt
     1741 cgctccgctg tgcttctcca gcgtccggag ctcggtttca agcgccagca gcgccgcatg
     1801 cgtcaggtct tcctgacgct caccggctga cactttaaca cctgacgact tcggcttttt
     1861 cagcgtcgat tcataatcct ttttcgccgc cgccatcagc gtgttgtaat ccgcctgcag
     1921 gattttcccg tctttcaggg ccttattcag ctcttcctgc cggttagtat atttctccag
     1981 cggcgtcagc agacgctcat acgccttctg cgcctctccg gtatacttca gctgtgatgc
     2041 gtcccgttcg gcccggtccc tggtagccag ttcaccggct ttttccatat ccgactgcag
     2101 cgtggccgct gccagcccca gacgggcatt ttcccggtca tcccatgcgc cctgcaggtt
     2161 ggcacggaaa gaggaggtct ttccccggcg ctggctccgg ctctggtacc actgccattt
     2221 tttatccgcc tcatcgaatg ccttctgcgc actggcaagc atatccgctg aggattccgg
     2281 acgaccaata tccagaatgg catcccacat cgatttgaac gccttccctg ttttatccgc
     2341 ccaggtctcc agcgtcccca tgttttcttt caggcgacgg gtctgctcat caaagccttt
     2401 cgtggcag
//
LOCUS       DAZGCS010000275         2398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGCS010000275 DAZGCS010000000
VERSION     DAZGCS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.280"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            25..534
                     /gene="eco"
                     /locus_tag="ACHK01_005193"
     CDS             25..534
                     /gene="eco"
                     /locus_tag="ACHK01_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO8099951.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(683..2329)
                     /gene="mqo"
                     /locus_tag="ACHK01_005194"
     CDS             complement(683..2329)
                     /gene="mqo"
                     /locus_tag="ACHK01_005194"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO8099952.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          566 a          664 c          601 g          567 t
ORIGIN      
        1 gacatcatcg gtgaaaacct ataaatgaag aaggaaagca aaaaaatgaa gaccattcta
       61 cctgcagtat tgtttgccgc tttcgctacc acttccgcct gggcggcaga aagcgtccag
      121 ccactggaaa aaatcgcgcc ttatccacaa gctgaaaaag ggatgaagcg tcaggtgatt
      181 cagttaaccc cgcaagaaga tgaatctacc ctgaaagtag aactgttaat cggtcagacg
      241 ctggaagtcg attgcaattt gcatcgtctc ggcgggaagc tggaaaacaa aacgctggaa
      301 ggctggggct atgattatta tgtctttgat aaagttagct cgccggtttc cacgatgatg
      361 gcctgcccgg atggcaagaa agagaagaaa tttgtcaccg cgtatctggg cgatgctgga
      421 atgctgcgtt acaacagcaa gctgccgatc gtggtgtata cgccagacaa tgtagatgtg
      481 aagtaccgcg tctggaaggc ggaagagaaa attgacaacg cggtagttcg ctaaaatgct
      541 gtgaagtgcg gcaccccgta ggccgaataa ggcggtcacg ccgcatccga catctaacgc
      601 ccgagccggt gcctgatgcg acgctggcgc gtcttatcag catacgccac atccggcata
      661 ccatgccgga tgtggcacat cattacaacg caatatccgc cacttctttt tgaacgggtt
      721 gcggtttcaa ctgcggtttt ggcgtactat ctgctgcctg cggcttgtcg tatttcagcc
      781 ccagcacttc gctggtgtac tgcaactcgc gttctgttgc cgctacatca ccgttcagct
      841 tgcgtccata agacggaacg atcgctttca acgtagcctg ccattgcggg ctggaaacgc
      901 gatcgccaaa tactttttcc agcagatcca acataatcgg cgcggcggtt gacgcccctg
      961 gcgatgcccc cagcagcgcg gcaatggtcc cttgctggtc actgaccact tcagtaccca
     1021 gacgcagtac accaccttta tcggcatcac gcttgataat ctgcacgcgc tgccccgctt
     1081 gccacaaacg ccagtcctct tttttcgctt gcggatagta ctctttcaac gcttcaaaac
     1141 gatcctcttc actcaacatc acctgactca ccagatattt caccagatcg aaattatcca
     1201 gcccgacgtg catcatcggc atcacgttag aggtggtggt ggaactcatc agatcccaca
     1261 atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa tggcccaaac agcactacgc
     1321 gtttaccgtc cagaacgcgg gtatcgatat gcggaaccga catcggtggt gcgccaacgg
     1381 atgctttacc gtaaaccttc gccagatggt gattaaccac gtccgggttt tccgaaacaa
     1441 ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc ttccggaatc cccgattcct
     1501 gtaatagctt cagcgccgca ccgcccgcgc cgataaagac aaatttggca cgaatgttct
     1561 gtgcagtgcc attttttaga tcggcaacgg taacggtcca ggtgttatcg tcattacgct
     1621 ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt agatttcttc tgcaaggaag
     1681 caattaactg gcgggtgatc tcgccgtagt tcacatcggt accaatttcc gtacgcgtgg
     1741 ctgccacttt ctgttgcgga tcgcgccctt ccatcactaa aggtgcccac tctttgatct
     1801 gcgcgtgatc ttcagagtaa cgcataccgc gaaacagcga gctttgttgc aacgcggcgt
     1861 aacgggcgcg caggaaatta acgttatcct cgccccagac aaagctcata tgcggaacgg
     1921 tattgataaa tgaacgcgga gtacgcagca cgccgcgctc gacctggtgc gcccagaact
     1981 ggcgggaaat ctgaaatgct tcgttaatgg cgactgcttt ttcaatactg atgctgccat
     2041 cggcgttttg cggggtgtag ttcagttcca tcagtgcaga atgcccggtt ccggcgttat
     2101 tccagccgtt cgaactctcc tgcgcgacac cctccaggcg ctccaccatg gtcatcgacc
     2161 attcaggctc cagctcgcgt aaataggtcc ccaacgtggc gctcataatg ccgccgccaa
     2221 tcaacagtac atcagtttcc tgctcctcgg acgctttcgc ttttgccgcc atcgaaacgg
     2281 cattaagccc cacggccatc gagaagagca tggcagtcac ttttttcatc ttgttaatgc
     2341 cttactttta gtcgctttat tgcaggtgag atttgcgcgg catcaacggt aacactta
//
LOCUS       DAZGCS010000276         2369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGCS010000276 DAZGCS010000000
VERSION     DAZGCS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.281"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2262
                     /locus_tag="ACHK01_005195"
     CDS             <1..2262
                     /locus_tag="ACHK01_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8099953.1"
                     /translation="LTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRA
                     YGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQ
                     HRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWD
                     GDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFY
                     HCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYY
                     NRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDS
                     YGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAI
                     TYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVM
                     TLACRTWQECWVN"
BASE COUNT          578 a          597 c          746 g          448 t
ORIGIN      
        1 ctgactaacg agaacggcag ccacagcgac ttcagctacg atgcgctgga ccggttggta
       61 cagcagggcg gctttgacgg gcggacgcaa cgttatcatt atgacctgac cggaaaactc
      121 acgcagagtg aagatgaggg gcttgtcacc ctctggtact acgatgaatc ggaccgtatc
      181 actcaccgca cggtgaacgg cgaaccggca gagcagtggc agtatgatga ccacggctgg
      241 ctgacagaca tcagccacct gagcgaaggc caccgtgtcg ctgtccacta cggctacgac
      301 gataagggcc gcctgaccgg cgaacggcag acggtggaga acccggagac gggggaactg
      361 ctgtggcagc atgagacaac acacgcctac aacgaacagg ggctggcaaa ccgcgtcacg
      421 ccggacagcc tgccgccggt ggagtggctg acctacggca gcggttatct ggcgggcatg
      481 aagcttggcg acacgccgct gctggagtac acgcgcgacc gcatgcaccg ggagacggtg
      541 cgcagctttg gcagcatggc aggcagtaat gccgcatata aactgaccag cacatacacc
      601 cccgcaggcc agttacagag ccagcacctg aacagcctgg tgtatgaccg tgcctacggg
      661 tggaatgaca acggcgacct ggtgcgcatc agcggcccgc gacagacgcg ggaatacggc
      721 tacagcgcca cgggcaggct ggagagcgtg cgtaccctcg caccggacct ggacatccgc
      781 atcccgtatg ccaccgaccc ggcgggaaac cgcctgccgg acccggagct gcacccggac
      841 agtaccctca cggcgtggcc ggataaccgc atcgcagagg atgcgcacta tgtttaccac
      901 tatgatgaat acggcagact gacggagaag acggacctta tcccggcggg tgttatccgg
      961 acggacgacg agcggaccca ccactaccac tacgacagcc agcaccgtct ggtgttctac
     1021 acgcggatac agcatggcga gccactggtc gagagccgct acctctacga cccgctggga
     1081 cggcgaatgg cgaaacgggt atggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
     1141 tcgcgtaaac cggagatgac gtggtacggg tgggacggcg accggctgac gacggtacag
     1201 accgacacca cgcgtatcca gacggtatat cagccgggga gcttcgcgcc gctcatccgc
     1261 atcgaaacag acaacggcga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
     1321 cagcaggaag ggagcgagga cgggcacggc gtggtatttc cggctgaact ggtgcggctg
     1381 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
     1441 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
     1501 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
     1561 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
     1621 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
     1681 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
     1741 acacaagacc ccattgggct ggcgggggga tggaacctgt ataattaccc actgaatccg
     1801 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
     1861 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
     1921 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
     1981 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
     2041 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     2101 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     2161 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     2221 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     2281 aatatatatt tttagtttta tttttattgt gtggttgttc tccagtggac atagagccag
     2341 ctagacaaga agttaatatc tttcgtggt
//
LOCUS       DAZGCS010000277         2345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGCS010000277 DAZGCS010000000
VERSION     DAZGCS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.282"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHK01_005196"
     CDS             complement(<1..348)
                     /locus_tag="ACHK01_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO8099954.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHK01_005197"
     CDS             complement(329..742)
                     /locus_tag="ACHK01_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8099955.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHK01_005198"
     CDS             complement(769..1200)
                     /locus_tag="ACHK01_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8099956.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHK01_005199"
     CDS             complement(1214..1966)
                     /locus_tag="ACHK01_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099957.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(1974..>2345)
                     /locus_tag="ACHK01_005200"
     CDS             complement(1974..>2345)
                     /locus_tag="ACHK01_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8099958.1"
                     /translation="AVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLTDAEYTGEE
                     LDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTMVQQGYDYR
                     RDDDMALWSSADLTYSITYEM"
BASE COUNT          529 a          741 c          548 g          527 t
ORIGIN      
        1 accggcaagc cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctggc ggctcaggcc ctgttcaaca gcactggcgg tttttctggc
      241 gtcggtatcc agacctgaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctaaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 cggaaatccc cgctgaagcc atcattaatg cggcatcatc caccatgaca tccgccacat
      421 ccgcagacga taaaatatcg cgccctccgt ccccaccgaa ccggacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttgtcct catccggcct ctccacctgc tcttcctcat
      541 gccgggggac aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacagc gtacgtcagc ccggaaaaat gcatatccag ctgggtatcg tgaaaataat
      661 gcatgcggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatttttcg ccacttcggt
      841 aatatccgta tccacatgaa caggcccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatcag caggccaggt ggtcaggact tcctgctcaa tctgcatcac
      961 agcctcattc attgacggtg accccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtatgacctc cgtttaccct gcagcgcctg cttcagtaac ggtgacttca gctaccgtgg
     1261 caaactgacc attaccggaa atcacgggga tactcacttt tccagcctta acccccgtca
     1321 cagtgatcac catatctttc acagcaatgg ttcccgttga cggatcggcg gaaaccgctc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac agtcagggtg gttgtttttc
     1441 ctttttccac ggtaccggat gtcggtgtca ccttaattgc agtggccggc gtaatttcgc
     1501 tgcgttcttc cgccacggaa ggtttaccca cgttggtcac tttcaccgta cgggtgatca
     1561 cttccttcgc cgttacggcc ttaccgatac tgctgaccca gccacggaac acatccaccg
     1621 tgccgttcgg gaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcgctccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtaggattc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgctgcc
     1861 agtcatcatc tgacaacggg tttgcataag catcaccctt gccggtgtaa acccacagag
     1921 tggtaccggc accttttacc ggcgccaggg gatttggtgt tggcatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt caaatctgca gagctccata acgccatatc gtcatcacga
     2041 cgatagtcat agccctgctg aaccatcgtg gtaatcatcc ctgccagtgc agggatcgcg
     2101 gacatcgccg gataaatccg ggactccatc cacgaatcca gctctgaatc cggcacctga
     2161 gcaggcagga aaacttcaat atgcagtgtg gcccgccagg tatccgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgaccgcgg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgc
//
LOCUS       DAZGCS010000278         2345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGCS010000278 DAZGCS010000000
VERSION     DAZGCS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.283"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHK01_005201"
     CDS             complement(<1..348)
                     /locus_tag="ACHK01_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO8099959.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTVGVV
                     EQNLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHK01_005202"
     CDS             complement(329..742)
                     /locus_tag="ACHK01_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8099960.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREKAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVADVSEDDVALMMASAGIPGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHK01_005203"
     CDS             complement(769..1200)
                     /locus_tag="ACHK01_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8099961.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHADTDSTEVAKYNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHK01_005204"
     CDS             complement(1214..1966)
                     /locus_tag="ACHK01_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8099962.1"
                     /translation="MSTPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRVVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(1974..>2345)
                     /locus_tag="ACHK01_005205"
     CDS             complement(1974..>2345)
                     /locus_tag="ACHK01_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8099963.1"
                     /translation="AVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLTDAEYTGEE
                     LDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALADLITTMVTQGYEYR
                     RDDDMALWSSADLTYSITYEM"
BASE COUNT          516 a          746 c          545 g          538 t
ORIGIN      
        1 accggcaagt cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctgac gactcaggtt ctgctcaacg acaccgacgg tttttctggc
      241 gtcagtctcc agtccggaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctcaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 ccggaatccc cgctgaagcc atcattaatg cgacatcatc ctcgctgaca tccgccacat
      421 ccgcagacga taaaatctca cgccctccgt ccccaccaaa ccgtacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttttcct catccggcat ctccgtctgc gctttctcac
      541 gccggggggc aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacggc gtacgtcagc ccggaaaaat gcatatccag ttgggtatcg tgaaaataat
      661 gcgtacggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatatttcg ccacttcggt
      841 gctgtcagta tccgcatgaa cagccccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatccg caggccaggt ggtgagcacc tcctgttcga tctgcatcac
      961 agcctcattc attgacggtg atgccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca gccactgcgg
     1261 cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc acgccgttta
     1321 ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg gaaaccactc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac cgtcagggtg gttgttttcc
     1441 cttttgccac cgtaccggat gtcggcgtca ccttaatcgc agtgaccggc gtaattttgc
     1501 tgcgttcttc cgctacagaa ggtttgccca cgttggtcac tttcaccgtg cgggtgatca
     1561 cttctttcgc cgtcacggcc ttaccgatac tgctgaccca gccacgaaac acatccaccg
     1621 tgccgttcgg aaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcggtccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtagggttc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgcagcc
     1861 agtcatcgtc tgacaacggg tttgcatagg cgtcaccctg accgttataa acccacaggg
     1921 tggtaccggc accttttacc ggctcaaggg gatttggtgt tgacatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt cagatccgca gaactccata acgccatatc gtcatcacga
     2041 cgatactcat agccctgcgt aaccatcgtc gtaatcaggt ctgccagtgc cgggatcgca
     2101 gtcatcgccg gataaatccg gctttccatc cacgaatcca gctccgaatc cggtacctgt
     2161 gctggtaaaa acacctcaat atgcagtgtg gcccgccagg tatctgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgacggccg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgc
//
LOCUS       DAZGCS010000279         2338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGCS010000279 DAZGCS010000000
VERSION     DAZGCS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.284"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            540..765
                     /gene="ykgL"
                     /locus_tag="ACHK01_005206"
                     /pseudo
     CDS             540..765
                     /gene="ykgL"
                     /locus_tag="ACHK01_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(800..940)
                     /gene="ykgO"
                     /locus_tag="ACHK01_005207"
     CDS             complement(800..940)
                     /gene="ykgO"
                     /locus_tag="ACHK01_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO8099964.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(940..1203)
                     /gene="ykgM"
                     /locus_tag="ACHK01_005208"
     CDS             complement(940..1203)
                     /gene="ykgM"
                     /locus_tag="ACHK01_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8099965.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1567..1668)
                     /gene="ykgR"
                     /locus_tag="ACHK01_005209"
     CDS             complement(1567..1668)
                     /gene="ykgR"
                     /locus_tag="ACHK01_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO8099966.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2142..2231)
                     /locus_tag="ACHK01_005210"
                     /pseudo
     CDS             complement(2142..2231)
                     /locus_tag="ACHK01_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          683 a          446 c          509 g          700 t
ORIGIN      
        1 aaagacattc agtttcataa ggttattggc aatataacgt tcaattctta cttgcatttt
       61 atattagcca ggaactttgc cctgaggtgt taggaatagt cttaatgtgt tgtgtaatgg
      121 actagtaaaa aaacaagttt aaatgataag aaatagaata taaaactatc atgagtcata
      181 attaaagatg atttgccaat cagtaaggtg ttttaatgga ttaacatgcc gcacaaaatg
      241 gttatgggtg ttcatcccaa taagtgatgc aaaattaaac aggtataagt acgtcatctg
      301 tccggtaagc cccctaagag ccaaattaag tgaagttaaa cgaagactgc cagttgtcac
      361 aggaacgatg gcctctttgc cagggcactg cattcagtca tctgtgcgcg ttactctaac
      421 tggcactcga acgtcggcga tggggttacc ggtggatcag gccgttcctg tggcgtaaac
      481 gtttttatgt cagtaccaac agatttacca catttatcat cttaacagtt tgagtgtaaa
      541 tgcaggctgc gtcgcaaagg gaggaaactg agtggcgtgt tcagtctaaa agagggttga
      601 tgccagcata cagggcgaag ctggtcaaca agtcaacatt aatataatgg aatattcgga
      661 gagaaacgtc aggcagttga ccttaaatga acaggaagat acatccccag aaaaattaat
      721 gctggtgtaa taaatacacc aacattaatc cggtctgatt attaatcaga caaatatcgt
      781 taattcgtcg aattttaaat caacgttttt tcttacgacc ctgaacggcc ttaaaacgtg
      841 gattagattt acaaatcaca tacagccgtc cttttcgctt cacaatctga cagtctggat
      901 ggcgttcttt tgcggtacgc agagagttaa ggactttcat catgccccct ttttcgtgct
      961 aacaaaacga ccaaaacgtt gggtgaaccg tgcaacattt ccttctgatg ccactgttct
     1021 cagcttccct gtatagaacg ggtgcgattt cgaagagaca tcaattgtca cgtatggata
     1081 cgttacgcca tccagctcaa tctcacggtc tgttttgata gtcgagccga ttttaaagta
     1141 ctcatcaaca ctggtgtcgt ggaacaccac agtacgatac tcaggatgga tattgggctt
     1201 catcattttt acctgttatg ttataacata accataaaga tacatgctca tccggatcat
     1261 ttcaacaccc aatccctgtg cccatttttg gtatgtggaa tttccccaac ggggaagtct
     1321 ggcattgtta gcagccgttg gcgacgctcc cataaacgcc tggatgagtg attgatgatg
     1381 gtttcgctga gagtatggat ttttcgacaa tggaggttat ctccacttta ctggccgatg
     1441 atttatcgca gtatgattga ttacctgaca gcgtgtattc ctctcccacg gatataataa
     1501 ttacttacaa aaagggagag gatgcatatt ttaaatatca ctgaagtgaa cagtttattg
     1561 ccgttattaa tagaaatgga gaaataaata ggcgtattct acaattgcga caaaaacaac
     1621 gatattaatc agtttatgac tgatttgttg tactttattc tctttcattg gtacttcctc
     1681 gctttaaaaa agagcgcact tcgtaagtgc ccttatataa ataacgagtt tggtcaacca
     1741 attttttgac atgtatcaca aatttgaatg gatgtattac atcggctatc ttttattgca
     1801 ccaacgtcat tgatatatgt cgcctgaagt cagttccggg aatgagtctg atctcaagac
     1861 tggcccagtc cgggcgttga ttggcgctga ggagcatatc gcatctcatc ataatgtcgt
     1921 atctcctggg gtgttataca agatatcgtt gttggtgacc tgggagagga attgagttct
     1981 attaaaccgt caactatgcc ggatacatac tggattacac tgcaggcacg ccttacgaga
     2041 gaacgtgccg cagtgacggg ttaattatct gaaagaattt gcgaggctgt atcggttact
     2101 cattggtttg atagttttac tctcgggaga gtaatagata tttaattcat taacggaaac
     2161 cagtcagttc ctttcgatgc ctgaatttga tcccatagtt tatccggaat ggttaattca
     2221 tgaacaagtt gtgttatcgt tcatgagaag cataacgtaa agggaaaagc tcgattagac
     2281 ggcaggattt gtcaggggtt atgaacgaaa ttcataaatc tgtttgagtg ttgcgatg
//
LOCUS       DAZGCS010000280         2316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGCS010000280 DAZGCS010000000
VERSION     DAZGCS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.285"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(558..992)
                     /gene="uspF"
                     /locus_tag="ACHK01_005211"
     CDS             complement(558..992)
                     /gene="uspF"
                     /locus_tag="ACHK01_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO8099967.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1133..2266)
                     /gene="ompN"
                     /locus_tag="ACHK01_005212"
     CDS             complement(1133..2266)
                     /gene="ompN"
                     /locus_tag="ACHK01_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO8099968.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          631 a          505 c          533 g          647 t
ORIGIN      
        1 ctctgtatca tcggtctcga aacccgtagc cacgtcgtat gcaagaacgt gccgcggttg
       61 gctggtaagc ttttcgatag tgcgagtatc taatgatttc cagccggaac tgattctatt
      121 ggaaagaaat catgggcgca atttacagat cacagattga ttagttatag atttttactg
      181 tggcaaaaat gggacaaaag gccgcaaaag gggcaaaaat ggggcaaaaa aagtgtagat
      241 tattgtgctt tattgtagtt gttaatcctg cctaacatat tgaaaaacat aaaagttatt
      301 gttcgtcaga tagttgcggt ttttactctg acttgttcag gttgtattgt tctttcttac
      361 taatttcttg attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag
      421 tatgcgtcat gccggaatgc ccagcataag aaatctgata taaaaacagt ggcgtgtatg
      481 gtacggatta gaggggaaaa tgtcagcaca tttgcgaaat gaatcaaaaa gcccgcagca
      541 atgtgcgggc gttagtgtca gcgcacaacc agcacggagc actctgcgtg acgcactaca
      601 gctgcggcgt tggaaccgag cagataagtg gtgatatccg gtcgatggga agcaatgatg
      661 atcatatgag cggggatctt cttcgccatt tccagaatgc ggtctttggg cgagccttcc
      721 tcaacatgga catgcactct gtcggttggc agtttaaatt ttttaatgat ctcttccagt
      781 tgcgatttgg cttccgcttt caggtcatcc attgccggta attctgcgga atacgctaaa
      841 cccagagagg catagtaggg cagtgaaggt attaccgtca ggaaatgaac ctctgcatca
      901 tcaatctttg cctctgcctc aacgtggcta atcacgcgtt gagttaattc tgaatcggaa
      961 atatcgatag ggacaagaat cgttctgttc ataaaacctc ctgttttagt atccgcataa
     1021 agtgtaacgc cagatgacac tttttgtgta atgacggagt tcacattttt aatttagatc
     1081 aaaggaggaa gaataagcag aaaaagcccg ccataacagc gggcaggagg atttagaact
     1141 gataaaccag acctaaagcg acaatatcat cggtagagat gccattggca gcgtagaaac
     1201 tgtcatcttc atccaacagg ttgattttat agtcaacata agtggacatg tttttattga
     1261 aatagtaagt cgcgccaaca tcggcgtatt taaccagatc tttatcatca acacctgccg
     1321 ggttgtctgc accaccggca gcgtgcaggt cacggccttt agacatcagg aaagagactg
     1381 ccggacgcag accaaaatca aactggtact gtgcagtgac ttcaaagttc tgggttttgt
     1441 ttgcaacagc ataatcgctg tcgccaaacg gggtcatatt acgcgtttct gaatacatgg
     1501 ttgccaggta aatattgtta gcatcgtatt ttagcccagc agtccacgcg tctgctttat
     1561 caccacccgc cgcagtatgg ttaacctgat cattggtgcg gtcagaagag gtgtatgccg
     1621 caccagcgct aaagcccatg cctaaatcat atgttgtgga aagaccccag ccgtcaccgt
     1681 tttcatggcg aacatcacgt ccgttgttgg tgccttcctg accattactg gcaccttcgt
     1741 tgttaccttg atactgcacc gcgaagttca gaccatttac cagaccgaag aaatcagtat
     1801 tacgataagt cgcgacgcca ttggctcgac cagtcataaa gttgtctgca ttggtataag
     1861 agtcaccgcc aaattcaggc agcatatcgg tccagccttc gatgtcgtac attacgccat
     1921 aattacgtcc gtaatcgaaa gaaccgtaat ctgcaaattt cagcccggca aatgccagac
     1981 gggtccatga ctggtttttt gaagattcag tgttgtttgc ctgaatattg tattcccatt
     2041 gaccgtagcc agtgagttga tcgttaattt gggtttcgcc tttaaaaccc agacgcgcat
     2101 agctctggtc gccatctttc gccgaattat cagaaaaata atgcaggcca tcaactttgc
     2161 catacagatc taatttgttg ccgtctttat tataaacttc ggctgcatgt gcagcacctg
     2221 cggcgagcag ggcaggaatt aaaagtgcca gtactttgct tttcattgaa taaatccttt
     2281 agttatttta tttgcctttt atccccattt cgggga
//
LOCUS       DAZGCS010000281         2281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGCS010000281 DAZGCS010000000
VERSION     DAZGCS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.286"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..111
                     /locus_tag="ACHK01_005213"
     CDS             <1..111
                     /locus_tag="ACHK01_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8099969.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPNQADF"
     gene            176..424
                     /locus_tag="ACHK01_005214"
     CDS             176..424
                     /locus_tag="ACHK01_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8099970.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            402..1532
                     /locus_tag="ACHK01_005215"
     CDS             402..1532
                     /locus_tag="ACHK01_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO8099971.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1578..2216)
                     /gene="ompW"
                     /locus_tag="ACHK01_005216"
     CDS             complement(1578..2216)
                     /gene="ompW"
                     /locus_tag="ACHK01_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO8099972.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT          666 a          544 c          543 g          528 t
ORIGIN      
        1 gctatcccat tcgaaggtga gcgccacaat gcgctggatg acgctcgtta ccaggcaaaa
       61 tacgtttcag ctatctggca aaaactgatc ccgaatcagg ctgattttta atgttcaacc
      121 ccggtcgttg cccaccagct atagtggcgg cgaccatgat tagcgaacga cgctcatggc
      181 aagacttatt ctgctcactg agtgggcaaa agaggaattc agtgaaccgg tccctactcc
      241 gagtacgtta agtaaatacg ctaaagccgg aatgatattt cctctcccca aaaaagttgg
      301 aagacgctgg cgagtggatc cgcaagctcg ctttgtcgga atggtaaaca agccggaggt
      361 gatcgccaca gatcaccctg ctttgaagag gatactggaa gatggcgcgc ccgcgaaaat
      421 ataaaaccga tgttccggga ttatctccgt attttgacaa aagaaataac aaagtttact
      481 ggcgttacag gcatcccata acaggcaaaa atcacggtct cggcagtatt gaccagaaac
      541 tggcagaaac tattgcagca gaagcgaaca gccgtcttgc ccggcagcaa atggaacaaa
      601 tgctcagtct gcaggagaaa attattagtg ataccggcgg ttcatcaacc gttaccattt
      661 ttctgaataa ttacagaaaa attcaacagg aaagatatga aaacggcgag atcaaactca
      721 acacgctgaa acagaaagcg gcccctctca gggtatttga tgaacgtttt ggcaccagac
      781 cgttagatgc cataaccgta aaagatgtgg tatcagtact ggaagagtac aaggccagag
      841 gacataacag aatgggacaa attttcagga aagtactgat cgatgttttc cgggaagctc
      901 agcaaacggg cgatgtcccg ccaggcttta accctgcaga atctgcaaaa aaaccgcagg
      961 tgcggatatc aagacagcga ctgacttttg atgagtggat gatgatttat aacgcagcgg
     1021 aaaaggatgg ttacttttta cagcgcggta tgctgctggc actgatgaca ggccagcgcc
     1081 tttcagatat ttgcaaaatg caattttcgg atatccggga tggttatctt catgtcgaac
     1141 agcaaaaaac aggaacccgg attgccatcc ctctggctct gcgttgcgat aaattaaatc
     1201 tcaccctgga tgatgtggtg tcatcctgcc gcgattgcgt tcttagtccg tggctattgc
     1261 accaccatca cgcgaaaggg acagctaagc gcggcgggat ggttaagcca gcaacattaa
     1321 ccgttgcatt taaaaaagcc cgggattctg tggattacaa ctggcgtgct aatggcaccc
     1381 caccctcttt ccatgagcag agatctttat cagagcgatt gttcagagag cagggggttg
     1441 ataccaaaat tttgctaggc cattcgaatc aaaaaatgac cgatatttac aacgacgcac
     1501 gcggtaagga atggaaaaaa ctggtcattt gatgaccagt tttgcagagg ggttttgcag
     1561 aggttttgca gagaaaatta aaaacgatat cctgcggaga acataaacac ccacggatcc
     1621 agtcgtaccg agtctttcac ggtagtaaca ccagatttat acttagccgt ggtatcgata
     1681 tccatgtacc acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc
     1741 acctgcccgg cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg
     1801 ccatgatcgt taaatccatt atcaaagaag gtggtgtagt taataccggc cccaacgtaa
     1861 ggacggaatt tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga
     1921 tggtgaacgg ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc
     1981 gctgccagta attccacacc aatgttgtcg gtcgccatat aagtaaacgt caagcccagt
     2041 tgagtgttat tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct
     2101 gttggacgta cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag
     2161 gcactgccag aaagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc
     2221 gctccgtcgt tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat
     2281 c
//
LOCUS       DAZGCS010000282         2274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGCS010000282 DAZGCS010000000
VERSION     DAZGCS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.287"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..222
                     /locus_tag="ACHK01_005217"
     CDS             7..222
                     /locus_tag="ACHK01_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8099973.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            226..783
                     /locus_tag="ACHK01_005218"
     CDS             226..783
                     /locus_tag="ACHK01_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099974.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNCSELEWECNRPSFVVSGDDGKITISENGKVTLPSHQ
                     HSETLIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDAIVREALLRN
                     FNTPPFKKVPAYWLHPGLTYLKVRI"
     gene            820..1353
                     /locus_tag="ACHK01_005219"
     CDS             820..1353
                     /locus_tag="ACHK01_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8099975.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1872..2057
                     /locus_tag="ACHK01_005220"
     CDS             1872..2057
                     /locus_tag="ACHK01_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8099976.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          587 a          505 c          653 g          529 t
ORIGIN      
        1 cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg
       61 ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca gtgggcggca
      121 ataggcgtgc tggggagtct gctgtttggt ctgctgacgt acctgacgaa cctgtatttc
      181 aagattaaag aagaccggcg taaggcggca cggggagagt aggtgatgaa ccatgaagaa
      241 atgaatcagc gcttcagtcg tctggaaaat gaaattgctg aactgaataa aaaactgtcg
      301 gcgctgatgc cttctgaaga tgaaaaaaaa cgccgcgatg agcagtttgc tgcgtttgac
      361 gattattgtc ggaaagtgat gagcagaaat ctcgcagagt gtttcagtat tcataatgat
      421 aattgcagtg agctggaatg ggagtgtaac cggccatcct ttgttgtatc cggtgatgac
      481 gggaaaatca ccatatcaga aaatggaaaa gtaacactgc catcgcacca gcacagtgag
      541 acgctcattg aatttgccat tgattacctg aagaacaata aaaaacaggg gctgatgcag
      601 cgcattggtc gttgcatggg gtatctgcag gtagccgctg agattgaagc gctggccagt
      661 ggtgctgata aggatgcaat tgtgcgggag gctcttcttc gtaattttaa tactccaccc
      721 tttaaaaaag tgccggctta ctggcttcat cccggactga cttatctgaa agtgcgtatt
      781 tagtgggcct gggacagcgg ctgaatattt aatatatcca tgaacacaaa aatcaaatac
      841 ggcctgtcgg ctgccgttct ggcgctgatt gccgcaggtg cttctgcgcc tgaaattctc
      901 gaccagtttc ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt
      961 atctggacca tctgccgtgg agccacccgg gtggatggta agcctgttat tcctggcatg
     1021 aagctgtcga aggggaaatg cgaccgggtt aacgccattg agcgtgataa ggcgctggca
     1081 tgggtggaga aaaacatcaa agtgccactg actgaacccc agaaagcggg gatcgcgtca
     1141 ttctgcccgt ataacattgg ccccggtaag tgtttcccgt cgacgtttta taaacgaatt
     1201 aatgcaggcg atcgcagggg agcgtgtgaa gcgattcgct ggtggattaa ggacggaggc
     1261 agagactgcc gtatccgctc aaataattgc tatggtcagg tatcccgtcg tgaccaggag
     1321 agcgcgctgg cgtgctgggg tatcgacaga taagcagaat attttgctga aaaatgacgt
     1381 tggccaacgc ggacggataa cacgaaatcc tgcgaactgg caaaacctga gtgaataaaa
     1441 gtaaaaaccc cgtttgttag cagcaggtgg ggttttgtgt ttcctgactc cggaaaagtc
     1501 aaaggagaaa gtgtgtttga ttttagcaaa ctgattcggg agatttgagt gatggctgaa
     1561 aaattatcta cctggaagtt cattcttatc tggctggtgt ttgtgattat ggtctccggt
     1621 tatttcatcg gtcagatacg ctggtggtga aatgaaccgc gtactgtgtg tggtcatcat
     1681 tgccctgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc gtgataacgc
     1741 catcacctac aaagcgcagc gcgataaaaa agccagagag ctggagctgg caaacgcaac
     1801 cattactgat atgcaggtgc gccagcgcga tgttgctgcg ctcgatgcaa aatactcaag
     1861 ggaattagct gatgcgagag ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg
     1921 taagcgcctg cggatcaacg ccacctgctc cggtaccgtg cgtgaagcca ccggcacctc
     1981 cggcgtggat aatgcaaccg gcccccgact ggcagacacc gctgaacggg attatttcat
     2041 cctcagagaa cggttgatga caatgcagaa gcagctggaa ggggcacagg actatatccg
     2101 cactcagtgc ctgaactaag ttttgcggat gcgccgtatc gtcgctgtat tccctcatta
     2161 acagagaccg cagcccgaca gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa
     2221 cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc atag
//
LOCUS       DAZGCS010000283         2250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGCS010000283 DAZGCS010000000
VERSION     DAZGCS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.288"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..110)
                     /locus_tag="ACHK01_005221"
     CDS             complement(<1..110)
                     /locus_tag="ACHK01_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8099977.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYG"
     gene            complement(94..>2250)
                     /locus_tag="ACHK01_005222"
     CDS             complement(94..>2250)
                     /locus_tag="ACHK01_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8099978.1"
                     /translation="TQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTL
                     SHTSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPD
                     SLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYT
                     PAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLD
                     IRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPT
                     GVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLIRVETENGEREKAQ
                     RRSLAEKLQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVER
                     LAAQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLH
                     QPYRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDP
                     MGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRH
                     CYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCS
                     DACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          411 a          709 c          568 g          562 t
ORIGIN      
        1 ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa
       61 caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc
      121 ttgctttgct tttattggat tatctgcttt tataccatct aacccgaaga gttgcccagt
      181 gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc
      241 gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc
      301 tgccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg tcttagtcat
      361 tcgacacatc aagtaacaat gtctcattgc atcggcggcc ccattatgag ctccgccaac
      421 aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt ttgcagcaat
      481 caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc
      541 atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca
      601 tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta
      661 ccggtgacgg ttatagtaca gccccgactc cttatcatac tgctgccccg gcagccggta
      721 cggctggtgc aggtgatgcg ggttctcctc attaagctgg ttgccccatt catcatattc
      781 cgcgctccac gccgtattgc cgtcttcact gataagcgcc agcggcaggc cccggtggtc
      841 acagtgataa agatgtattt ttcgctcagg caaataaacc ggctctatct gtgccgccag
      901 ccgctccacc gtcagcccgc actgcgcaag ccactggcgg ctttcactgc tcacgcagtt
      961 tgcccggatt tccccctcca gcctgtccag cagccccacc agttcagccg ggaacaccac
     1021 accgtgaccg tcttcgctcc cttcctgctg taacttctcc gccaggctgc ggcgctgcgc
     1081 tttctcccgc tcgccgttct ctgtctcgac ccggatgagc ggcgtgaagc tccccggctg
     1141 gtataccgtc tggatacgcg tggtgtcggt ctgtaccgtc gtcagcctgt cgccgtccca
     1201 gccgtaccat gtctcctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc
     1261 ccgccgccat acccgtttcg ccattcgccg tcccagcggg tcgtagaggt agcggctctc
     1321 gaccagtggc tcgccatgct gtatccgcgt gtggaacacc aggcggtgct ggctgtcgta
     1381 gtggtagtgg tgggtccgct cgtcgtccgt ccgtatcaca cccgtcggga tacggtccgt
     1441 cttctccgtc agtctgccgt attcatcata gtggtaaaca tagtgcgcat cctctgcgat
     1501 gcggttatcc ggccacgccg tgagcgtgct gtccgggtgt agctccgggt ccggcaggcg
     1561 gtttcccgcc gggtcggtgg catacgggat gcggatgtcc aggtccggtg cgagggtgcg
     1621 cacgctctcc agcctgcccg tggcgctgta gccgtattcc cgcgtctgtc gcggaccgct
     1681 gatgcgcacc aggtcgccgt tgtcattcca cccgtagtca cggtcatata ccaggctgtt
     1741 caggtgctgg ctctgtaact ggcctgcggg ggtgtatgtg ctggtcagtt cgtatgcggc
     1801 attactgcct gccctgctgc cgaagctgcg caccgtctca cggtgcagcc tgtcccgcgt
     1861 atactcgacc agcggcgtcc cgcccagctt catgcccgcc aggtaaccgc tgccgtaggt
     1921 cagccactcc accggcggca ggctgtccgg cgtgacgcgg tttgccagcc cctgttcgtt
     1981 gtaggcgtgt ttcgtctcat gctgccacag cagttccccc gtctccgggt tctccaccgt
     2041 ctgccgttcg cccgtcaggc ggcccttatc gtcgtagccg tagtggaccg acacgcggtg
     2101 gccttcactg gtatggctga gggtggttaa ccacccgtgc tcatcatact gccactgctc
     2161 tgccgggtcg ccgttcaccg tccggtgcgt gatgcggtcc gacgcatcgt agtgccagag
     2221 ggtgataagc ccctcgtctt cactctgtgt
//
LOCUS       DAZGCS010000284         2233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGCS010000284 DAZGCS010000000
VERSION     DAZGCS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.289"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..873
                     /locus_tag="ACHK01_005223"
     CDS             <1..873
                     /locus_tag="ACHK01_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8099979.1"
                     /translation="VDSIDNDSQIENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFE
                     GINLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPT
                     SVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFER
                     QRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIA
                     NSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYLEPDEGDNYLIK
                     DGANFVIQFNGVEF"
     gene            875..>2233
                     /locus_tag="ACHK01_005224"
     CDS             875..>2233
                     /locus_tag="ACHK01_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO8099980.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRC
                     RNIDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLP
                     SNNDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGND
                     PHH"
BASE COUNT          756 a          339 c          418 g          720 t
ORIGIN      
        1 gttgattcaa tagataatga ttcacaaatt gaaaatgtgt ttaatttgct cgatagttta
       61 tatgttgagt cagttgatac cctaactttc aaatatcaat caataataaa aggtctcgat
      121 agattatttg aaggtataaa cttagattca gcattctcat tatctgaaga agaacgctca
      181 tattttgaag aaaaagctaa aagtttaaac gcgcttgcac agttaggtat tagtgttgag
      241 ataatatctc atgaacttga agaaatggat tctatggtaa ccagaggact aaactctctt
      301 cctacttctg taaaagaaca ccctggtttt tcattggcgt taaatgctca cagatcgctt
      361 actcaacaaa tacgtttctt atcacctttg aaaatatcag gttatcaatc caggcagaga
      421 ataactggaa aaaatatcat ggattatgtc ctgaagttct ttggggagcg tttcgaacgg
      481 caacgaataa ctattgaatt tagtgaagag tttaagcaaa tcgcaataac agatatacca
      541 tcaaggatct atcctgtttt tactaatatt atcaacaatg caatgtattg ggtcagtctg
      601 tcaaataata ggctcataaa gattggtttt gtgaattctt tggttatcat agcaaattct
      661 ggtccggcaa ttgataccga tgatatcccg cgactatttg aactatttta tagcaaaaga
      721 gcaaatggac atggggtagg tctgtatcta tgtcgagaaa accttgctgt tgcacatcat
      781 aaaatatggt atttagaacc tgatgaaggc gataactatt taataaaaga tggcgctaat
      841 tttgtgatcc agttcaatgg agtggagttc taatatgaca gtggcaaatt ataattctct
      901 tgtccagaaa actttctgcg aaaatgcaat tcgttccgtt gtcatgattg atgacgattt
      961 tctgacgtat tctgaatcaa tcagggcgtt gaataacgaa gttgatttag actacaacaa
     1021 aattgactca tctaaacgag ccgctactct tgagagcttt tttcaatcta aaaatatgat
     1081 ttgtgatgtt gacaatggtt ctgttaattt cgatgtggat cggattagaa aatcagatct
     1141 tattattgta gattatcatc ttgataataa tgcacctgat aaaacactta aactattaca
     1201 agatttgaaa gactccgatc atttaaatat gattgtaata tatactagag agaatttaga
     1261 aacggtttgg atgcagatat catcgactct caaaggtgct ctggatatca acagcttgat
     1321 cattgactac gataatgaag atgtccaaag ttattgggaa gacgttgtat taccgaactt
     1381 aaatgataat ggtaataaag ctctcacaag agatgaaaca atagcctata ttaaagacag
     1441 taagccttgt agaagaatta aaagattaat acatgatgat gctgtgttgg aggatcaaaa
     1501 ggataaaaac ttcattgcaa aaatgattgc agaatatgct gtgtctagaa atgcaattat
     1561 ttctagcaac acatctggca atgtcattcg gggtgatgaa agcggagtaa aatggattca
     1621 atgtggtaat atctttgtct ccctatttca taaggttcaa gatgatcatg aaaacgatgg
     1681 agataggatt tggcaaactc tcaatgattc tctcattgaa tggaaaccat cttattatca
     1741 gttaataaaa tctgaaattc agaatgcaat cgaagctgag gctttatctt ttgtaaatca
     1801 tttggctaac gatcattacg gtcaagctgc gtggttaaat gagatattaa aatcagactc
     1861 gcctgatatt agatgtagaa atattgactt tgtatttggt aatttatcag aagagcttta
     1921 tcaaagactt aaaaataata atacgctgga tgaatttatc aaaagtgttt ttgatagcta
     1981 ttcaaatgaa tacgctaaca gcggagttgc tgcattgctc caatattgct cttcaaaaat
     2041 ggatctgcca tcaaataatg atacttatca cgaaatgtat catgctttaa atatgaattt
     2101 gtcttcaaag aattttgaag atggtcatat ttctactggc actattttct ttgatacaga
     2161 gtcgaacaaa tggtatttat gtgtatctgc ggcatgtgat ttggttccta ctcagggtaa
     2221 cgaccctcac cat
//
LOCUS       DAZGCS010000285         2229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGCS010000285 DAZGCS010000000
VERSION     DAZGCS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.290"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..404)
                     /locus_tag="ACHK01_005225"
     CDS             complement(<1..404)
                     /locus_tag="ACHK01_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8099981.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEV"
     gene            complement(465..1112)
                     /locus_tag="ACHK01_005226"
     CDS             complement(465..1112)
                     /locus_tag="ACHK01_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8099982.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGC"
     gene            complement(1010..1753)
                     /locus_tag="ACHK01_005227"
     CDS             complement(1010..1753)
                     /locus_tag="ACHK01_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8099983.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            complement(1759..>2229)
                     /locus_tag="ACHK01_005228"
     CDS             complement(1759..>2229)
                     /locus_tag="ACHK01_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8099984.1"
                     /translation="PTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKVYARFLDAVNFVN
                     GNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYR
                     GDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          469 a          748 c          556 g          456 t
ORIGIN      
        1 acttccgacg gattccggtc cccctttgag gtggtttcca ccagtgcctg cacaccgaag
       61 gtaaagcgca gtcggtcgat gtttgccgac gtgatggcgc gggtgatcgg cgtgtcatat
      121 ttcacttctg taccgagcac cgtctcggag ccggaggatt caaatccctc cggcggtgtc
      181 tgctcctgct caccggcccg gaacaccacc gtgacgccgg atatattggt attcccctca
      241 ctgtccagca ccggcgtact gttcagcagc acgcttttta atccatccac cggaccttca
      301 accggccctt cgctgatggc atcgatcaca ctcagcagct gcgtggactt caggttgtcc
      361 ttcgcttcgc gcggggtatg ccccttactg ctgcctttac ccattcctca cgctccataa
      421 acgataaaac cgcccggagg cggtttcaca taaaacattt tgcatcagca accaatcacc
      481 acaacctgac caccatcccc ttcgtctgcc gtgctgatct cctgagaaac cacacgtgac
      541 cccacgcgca tttcaccgta cagaacaggc agaacattgc cctgggcaac catgttatcc
      601 agtgaggaga aataggtgtt ctgtttgccg ttatctgtac tggctgccgt gggcgtcctg
      661 gctttcggtg ccagcatctg cgccactccg cccaggatca tactggcccc tgccgcatac
      721 atgcccgata cagccgcggc ccccagccag cccacagggt tccaccatgc caccgcaatc
      781 agcgccgccc caagcaccac ctgaaacaca ccgccacttt tagctcccgc cagacgcggc
      841 acgatgtgga tcacggcacc atttgccagc ggctcattaa gacgggcaga taattcattt
      901 tcgcctgcat cacgcccggc aatgcgcacc tgataccagc cctcattcag tttctgacga
      961 aacaccggga gctgtgtggc cagtgcccgg atggcttcag cccccgtttt cacacgaagg
     1021 tcgatacggc gaccaaatcg ttgcaaatcc ccgtaaaggc agatgcgtgc catgcccggt
     1081 gacgccagag ggagtgtgtg cgtcgctgcc atttgtcggt gtacctctct cgtttactca
     1141 gttgttcagg aatatggtgc agcagctcgc cgtcgccaca gtaaatggcg gcatgattcg
     1201 gcaccgatga accaaaacag cacagcagca catcgcccgg ctgggccgct gacaacggca
     1261 cctgatacaa ccccgttgcc tccatattat ccagatagag attctggccg tgacgccacc
     1321 agtcatcccc gcgatgaaaa tccggcatct caattcccgc cagatggtaa gcgtcccgga
     1381 acagcgtgta acagtccgtc accccgtgct caaagcgccg cccggtgaga tgcggcacac
     1441 agcggaattt atgaatcgcc ccccggcaga ccagccacca cggcaaatca ctctgcacct
     1501 gcagccgccg gtcggcctca ctcagccagg gcagaccacc ggggtggctg tggaccagcg
     1561 ccacaatctc tccctgcatt tctgcactca gccagtcctc cggcgacatc cggaaatacg
     1621 cctccggctc accagagata ttcacgcagg gaaaatatct ttccccctcc ggtgttctca
     1681 ccacgaagcc gcacgactcc gctggcgcac atcgccgggc gtgcgccaga atcgctgatt
     1741 ctgtctctgt catgggattc actgcgaaag tttgttaatg gaaaggaagc cgccaaagtt
     1801 gccgacgtta ttgcgaaact tacagccgct caggcatttg ctgcatttat ccttcgtgat
     1861 atcggacgtc ggctgatcat attcatccgc gaccgccgga ccgtgataac cgcactcgtc
     1921 accgcgatag gtccaggtgc aggtgttggc cagcatgatg cgccccggaa aaacggcacc
     1981 gtccgtttcc gtcggcgtgg acagtacaaa agaggcactc accgcgctca gttcgctgca
     2041 ctgctcgatg cgccagcggc tgatcacctc ctgctccgga tcggcgtaac tgtttccgtt
     2101 gacgaagttc accgcatcca gaaaacgggc gtaaacctta cgccggacca ccgttccgcc
     2161 gaccagactc tgcatatctt ccgccatccc ggtgaccata ccgtacaggt tagaaaccgt
     2221 cagcgtggg
//
LOCUS       DAZGCS010000286         2196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGCS010000286 DAZGCS010000000
VERSION     DAZGCS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.291"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2028
                     /locus_tag="ACHK01_005229"
     CDS             <1..2028
                     /locus_tag="ACHK01_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8099985.1"
                     /translation="HIVEALMKTPEINAMYPERKLFAIGWVKHKCKPGTKWPEIQAEL
                     RNWKKRQDAERKETGKYTSVVDLARAKANRQHTENPAEKIPPVTAAIHREYKQTWKTL
                     DRELAYYLWPGDADAGNIDGSILRWAKNEVIARDREDWKRISASMRKQPDALRYSRQT
                     IFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGRNQSATDTLSSPVPETDAVET
                     AIQDNEKTECKVEVESSVEREGPFYFLFSDKDGEKYGRANKLSGLNKALAAGATEITK
                     EEYFARKNGTYSGSQQNTGASDTIAQPEPVKVTADEVNKIMQAANISQPDADKLLAVS
                     RGEFVAGISDPNDPKWVKEIETRDSVNQNQQETEQNDQKAEQNSPNALQNEPETKQSE
                     PVVQQEPEKVCTACGQSGGGNCPDCGAVMGNATYQETFDEENQVEAQENDPEEMEGAE
                     HPHKENTGGNQHHASDSETGEAADPLIKVNGHHNLTSTSRAGIHLMIDIETMGKNPNA
                     AIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDVIKWWLKQSREAQSAILTDEI
                     PLDDALLQLREFIAENSGEFFVQVWGNGANFDNVILRRSYERQGIPCPWHYHNDRDVR
                     TIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            2098..>2196
                     /gene="xisR"
                     /locus_tag="ACHK01_005230"
     CDS             2098..>2196
                     /gene="xisR"
                     /locus_tag="ACHK01_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8099986.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKA"
BASE COUNT          690 a          524 c          547 g          435 t
ORIGIN      
        1 catatcgttg aagcactgat gaaaacgcct gaaattaacg ctatgtatcc ggaacgcaaa
       61 ctgttcgcta tcggatgggt taaacacaaa tgtaagccgg gtaccaaatg gcccgaaatt
      121 caggctgaat tacgtaactg gaaaaaacgg caggacgcag agcgcaaaga gactggaaaa
      181 tacacgtctg ttgttgatct tgcccgcgcc aaagccaacc gacagcacac tgaaaaccca
      241 gcagaaaaaa tccctcctgt aactgccgcc attcatcgcg aatacaagca gacatggaaa
      301 accctggaca gggagctggc ctactatctc tggcctggtg atgcggatgc cggaaacatt
      361 gacggcagca tccttcgctg ggctaaaaat gaagttatcg ccagagatcg cgaagactgg
      421 aagcgcatct ccgcatcaat gcgcaaacaa cctgatgcgc ttcgctacag ccgccagact
      481 atttttggcc ttgtccgtga acgtccgatc gacattcaca aagaccctgt ggcactgaac
      541 aaatacatta ctgaatacct gactacaaag ggcgtgtttg aagatgaagg aagaaatcag
      601 agcgcaactg atactctctc gtcgccagta ccagaaactg atgcagtgga aacggcaatt
      661 caggacaacg aaaaaaccga atgcaaagtg gaagtcgaat catctgtaga gcgtgagggg
      721 ccgttctact tcctcttcag cgacaaggat ggcgaaaaat acggtcgtgc aaacaaactt
      781 tctggtctga ataaggcgct ggctgcaggg gctactgaaa tcacgaaaga agaatatttt
      841 gcccgcaaaa acggtacata ctcaggttca caacaaaata ctggtgcatc tgacacgatc
      901 gcacaaccgg agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc
      961 aatatcagcc agcctgacgc cgataagttg cttgctgtat cacgtggtga atttgttgca
     1021 gggattagcg acccgaatga tccgaaatgg gtgaaggaga ttgaaacccg cgattcagtg
     1081 aatcagaacc agcaagaaac ggaacagaac gaccagaaag cggaacaaaa cagcccaaat
     1141 gcgttacaaa acgagccaga aacgaaacaa tccgaaccag tagtgcaaca ggaaccggaa
     1201 aaagtctgca ccgcctgcgg tcagagcggt ggcggcaact gccccgattg tggcgctgtc
     1261 atgggcaacg caacatacca ggaaacattc gatgaagaga atcaggttga agctcaggag
     1321 aatgatccgg aggaaatgga aggcgcagaa catccacaca aggagaacac tggcggcaat
     1381 cagcatcacg ccagcgatag tgaaactggc gaggcggcag atcccttaat taaggtgaat
     1441 ggtcatcaca atctcacatc caccagcaga gcggggattc atctgatgat cgacattgaa
     1501 accatgggaa aaaatcccaa tgccgcgatt atctcaatag gcgcaatatt tttcgatcca
     1561 caaaccggag atatgggacc ggaatttagc aagaccatcg atctggatac tgctggcgga
     1621 gttattgatc gtgacgtcat taaatggtgg ctgaagcaat cacgtgaagc gcagtctgcc
     1681 attctgaccg atgaaatccc gttagatgat gcactactgc aattgcggga atttatcgcc
     1741 gaaaactccg gtgaattttt tgttcaggtc tggggaaatg gagccaactt cgacaacgtg
     1801 attttacgcc gttcatacga acggcagggg atcccctgcc cctggcacta ccacaacgat
     1861 cgcgatgtac gcacaatcgt tgagctaggg aaagccatag acttcgatgc cagaacagct
     1921 atcccattcg aaggtgagcg ccacaatgca cttgatgacg cccgttacca ggcaaaatac
     1981 gtttcagcta tctggcaaaa actgatcccg agtcaggctg atttttaatg ttcaaccatc
     2041 gccggttgtg actggtattc tgcaaccggc cctcatctga tgtaagagat aaaagcgatg
     2101 agcgaagtaa tcatgattgt ctctcccggt aaatgggtat ccgaagagca gttaattgcg
     2161 ctgaaaggaa taaaaaaagg tacgttaaaa aaggcc
//
LOCUS       DAZGCS010000287         2193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGCS010000287 DAZGCS010000000
VERSION     DAZGCS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.292"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..309
                     /locus_tag="ACHK01_005231"
                     /pseudo
     CDS             92..309
                     /locus_tag="ACHK01_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            419..682
                     /locus_tag="ACHK01_005232"
                     /pseudo
     CDS             419..682
                     /locus_tag="ACHK01_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            906..1031
                     /locus_tag="ACHK01_005233"
                     /pseudo
     CDS             906..1031
                     /locus_tag="ACHK01_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1091..1597)
                     /gene="yciE"
                     /locus_tag="ACHK01_005234"
     CDS             complement(1091..1597)
                     /gene="yciE"
                     /locus_tag="ACHK01_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8099987.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKIAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1643..2143)
                     /gene="yciF"
                     /locus_tag="ACHK01_005235"
     CDS             complement(1643..2143)
                     /gene="yciF"
                     /locus_tag="ACHK01_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8099988.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
BASE COUNT          590 a          464 c          466 g          673 t
ORIGIN      
        1 tacagatgcg tacaaatgat ttttattcat tattaaacag aaaatattta taagtgctat
       61 attcatgaat gaaaaatagc tatatgcgta cattctcaac ctgcccgtca cgtagcaggt
      121 tgaatcactt tttcttgata aagggctggt cctgccaccg ttgttgttgg gtgcgttaaa
      181 aatacactac taatatgccc ggcatgcggg caacagagtc ctgttcacga tcgccgccac
      241 cggaaatggc gctgcctcga tacctgccag ttcatggcac tattcgaagc cgatgttccc
      301 cacgttatat ggagtaatca ttggggttat gccgcagcac gttgtatgca agaatgtgct
      361 gcggctggtt tgtgagcttt cgatagtggt tgttattttt gcccttattt gttccggagg
      421 ccctggttca atggtccgtc tgccccctgt ggtgatgtca gcaaaatcag ccactgcgcg
      481 aaccacaata gcccgggaag atgctgaaga tcaccaggta aagctgtcag cgcagaaact
      541 ggaagaactg ctcgcatcaa tggttaagga tgaggttgat cgcaatgatg ggatttattg
      601 acgtcagcga gagcagaagg aagaactgaa taacctgaat gatttacgct caatcagagc
      661 gatgatgatt ggcagtaatt agcggtcaat ttcgggtttt ttggctgtgc gctccccgcc
      721 cagcattgcg cgtcaattag ccccaaaaaa aacgaaaaag ttacaatcaa gtaaaaaatt
      781 ccttctgcaa aactaaaaaa gatttcaata aaatcaggca gttaaaaaca taaaaaaaag
      841 gtacaaaaaa gcatctcaaa gtcttgtttt ttgcttcaaa tacaattggt tattgtgttt
      901 tttgaaacct accagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
      961 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     1021 atcgttttta attccacaaa aaaacgaccc cgtaatatac ggggtcaata aggacatggc
     1081 ataaagtgta ttatttcttc gcttctacgc cgtcagtttc agagcgaatt aaaaatttct
     1141 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     1201 tcgcttcgat ggttggaatt gaagctgtat caccggcatt ttttgctgct gctaatagtg
     1261 aggtgtaaca ggcgatttca aattgctcga agacatatcc gctaatagag cctttgacta
     1321 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagctatt ttactcatgg
     1381 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     1441 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     1501 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     1561 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     1621 actaaaagtt aaagttcata tttcaggctt tattttcggc tttcttattt acgttattaa
     1681 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtc tctttcagaa
     1741 gcttcgctgc tttacgataa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     1801 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     1861 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     1921 atttcatgcg cttaattttc agattcgatt ctgattccac aacttgatca atacgttcaa
     1981 tctgtccatg tgtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcgtttgatg
     2041 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     2101 aaagcaggtg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     2161 tcactgcgag ccatacggcc cgcagatgca att
//
LOCUS       DAZGCS010000288         2186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGCS010000288 DAZGCS010000000
VERSION     DAZGCS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.293"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            387..932
                     /locus_tag="ACHK01_005236"
     CDS             387..932
                     /locus_tag="ACHK01_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO8099989.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            907..>2186
                     /locus_tag="ACHK01_005237"
     CDS             907..>2186
                     /locus_tag="ACHK01_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8099990.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQ"
BASE COUNT          518 a          509 c          624 g          535 t
ORIGIN      
        1 tgttgcccca tattcagtgt tgctgatttg tattatctga agttgctttt acgttaattt
       61 gatgcagatc aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg
      121 cgtagatgca cgttgtgaca tgtagatgat aattattatc attttgcggg tcctttccgg
      181 cgatccgacc ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc
      241 ggtttaaggt gtttccgttc ttcttcgtcg taacttaatg tttttattta aaacaccccc
      301 tgaaaagaaa ggaaacgaca ggtgctgaaa acgggctttt tggcctctgt cgtttccttt
      361 ctctgttttt gtccgtggaa tgaacaatgg aagtcaacaa aaagcagctg gctgacattt
      421 tcggtgcgag tatccgtacc attcagaact ggcaggaaca gggaatgccc gttctgcgag
      481 gcggtggcaa gggtaatgag gtgctttatg actctgccgc cgtcataaaa tggtatgccg
      541 aaagggatgc tgaaattgag aacgaaaagc tgcgccggga ggttgaagaa ctgcggcagg
      601 ccagcgaggc agatctccag ccaggaacta ttgagtacga acgccatcga cttacgcgtg
      661 cgcaggccga cgcacaggaa ctgaagaatg ccagagactc cgctgaagtg gtggaaaccg
      721 cattctgtac tttcgtgttg tcgcggatcg caggtgaaat tgccagtatt ctcgacgggc
      781 tccccctgtc ggtgcagcgg cgttttccgg aactggaaaa ccgacatgtt gatttcctga
      841 aacgggatat catcaaagcc atgaacaaag cagccgcgct ggatgaactg ataccggggt
      901 tgctgagtga atatatcgaa cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg
      961 cttcgctcac tgttcaggcc ggagccacag accgccgttg aatgggcgga tgctaattac
     1021 tatctcccga aagaatccgc ataccaggaa gggcgctggg aaacactgcc ctttcagcgg
     1081 gccatcatga atgcgatggg cagcgactac atccgtgagg tgaatgtggt gaagtctgcc
     1141 cgtgtcggtt attccaaaat gctgctgggt gtttatgcct actttataga gcataagcag
     1201 cgcaacaccc ttatctggtt gccgacggat ggtgatgccg agaactttat gaaaacccac
     1261 gttgagccga ctattcgtga tattccgtcg ctgctggcgc tggccccgtg gtatggcaaa
     1321 aagcaccggg ataacacgct caccatgaag cgtttcacta atgggcgtgg cttctggtgc
     1381 ctgggcggta aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat
     1441 gaacttgctg cttttgatga tgatattgaa caggaaggct ctccgacgtt cctgggtgac
     1501 aagcgtattg aaggctcggt ctggccaaag tccatccgtg gctccacgcc aaaagtgaga
     1561 ggcacctgtc agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt
     1621 gcctgcccgc attgcgggga ggagcagtat cttaaatttg gcgacaaaga gacgccgttt
     1681 ggcctcaaat ggacgccgga tgacccctcc agcgtgtttt atctctgcga gcataatgcc
     1741 tgcgtcatcc gccagcagga gctggacttt actgatgccc gttatatctg cgaaaagacc
     1801 gggatctgga cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgagcca
     1861 cctgacagtg tgacctttca catctggaca gcgtacagcc cgttcaccac ctgggtgcag
     1921 attgtcaaag actggatgaa aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac
     1981 accacgctcg gtgagacgtg ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg
     2041 gcagagcgga aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc
     2101 ggtatcgact cccagctgga ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag
     2161 gaaagctggc tgattgaccg gcagat
//
LOCUS       DAZGCS010000289         2125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGCS010000289 DAZGCS010000000
VERSION     DAZGCS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.294"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..615
                     /locus_tag="ACHK01_005238"
     CDS             <1..615
                     /locus_tag="ACHK01_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8099991.1"
                     /translation="AKIPVPADGKSITPDDVRPMIEQMVKEAVSHIPVPRDGRDYDPD
                     VLQKAVLEAVSALPAPQDGRDAMALEILPAIDDQKSFPRGSYATHQGGLWRAYEKTYG
                     MRGWECLVDGVADIDVSMTGERSFSVVVRQSSGQRTEKTFSLPVMLYRGVFRTGETYH
                     PGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            612..938
                     /locus_tag="ACHK01_005239"
     CDS             612..938
                     /locus_tag="ACHK01_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8099992.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            949..1299
                     /locus_tag="ACHK01_005240"
     CDS             949..1299
                     /locus_tag="ACHK01_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8099993.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            1296..1742
                     /locus_tag="ACHK01_005241"
     CDS             1296..1742
                     /locus_tag="ACHK01_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO8099994.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANELKDE
                     VVSRAPVRRGKLRRNVVVLSWRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1739..2083
                     /locus_tag="ACHK01_005242"
     CDS             1739..2083
                     /locus_tag="ACHK01_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO8099995.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
BASE COUNT          436 a          510 c          728 g          451 t
ORIGIN      
        1 gcgaaaatac cggtaccggc agacggtaaa agtatcaccc ccgatgatgt gcgtccgatg
       61 attgagcaga tggtgaaaga ggcagtgagc catattcctg ttccgcgtga tggtcgtgac
      121 tacgatccgg atgttctgca gaaggcggtt ctggaggcgg tgagtgccct gccggctccg
      181 caggacgggc gtgatgccat ggcactggaa atactccccg ccattgacga tcaaaaatcc
      241 tttccccggg gctcgtatgc cacacaccag ggtggactct ggcgggcgta tgaaaaaacg
      301 tacgggatgc ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagcatg
      361 acgggtgaac ggtcgttctc tgtggtggtc cggcagagca gtggccagcg tacggaaaaa
      421 acattttccc tgccggtgat gctctaccgt ggtgtgttca gaaccggcga aacttaccac
      481 cccggcgata cggtgacgtg ggggggctcg ttgtggcact gcaacagtat gaccggtgat
      541 aagcccggag aagctcattc atcaggctgg actctggctg caaaacgtgg gcgggatgca
      601 ggaggtggaa agtgacagca ttactgacac tggaagaaat caaggcacat ctgcgtgttg
      661 accatgacgc ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtgctgc
      721 tggcctacat ccagggcagc cgggataaag tgattagtga ggacggtgag ctgatccctg
      781 gtgaggcatt aacccggatg aagggggctg ccatgcgact gaccgggatg ctgtaccgga
      841 atccggatct tgctgagcgg gaagacctcg tgcaggggga actgccgttt tctgtttccg
      901 tgctgattta cgatttgcgt tgtccgacgg tgttatgagg agggggggat ggcaatatct
      961 gcaggtcgtc tgacacagat gatcagtgtt ctgaacccgg tgttaacccg taatgctgcc
     1021 ggagaaatga cggaagaatg ggtgtcatgc gggaaaattc atgcggatat ccggggcagg
     1081 agcagccggg agcggatgca gtccggtgcg gaaatggcgc aggcggaaat ccgcatctgg
     1141 gtgcgcggtc agtctggtcg tgaaatcacg gcggcgtcac gacttcatgt gctgagtggt
     1201 ccctggcgtg accatgtcct gaatgtcgtc ggggtcccgg ttccggatgc aaccggcggg
     1261 cgtctggaaa ttctctgtcg gctgggaggg gaaaaatgat cgaaaccctg ctggattttt
     1321 cggggctgga ggacatcagc cgcgatttgc agcttctgag tggtgcggaa aacaaccggg
     1381 tgttgcgtga ggcaacccgc gcgggggcga atgaactgaa agacgaagtg gtgtcacggg
     1441 caccggtgcg caggggaaaa ctgcgccgca atgtggtggt cctttcctgg cgttcccgcg
     1501 atggcgggat ggaatccggt gtgcatatcc gtggtgttaa tccggacacc ggcaacagtg
     1561 ataacaccat gaaggcggat aatccccgca atgccttcta ctggcggttt gtggaaatgg
     1621 ggaccgtgaa tatgccaccg cacccgtttg tgcgcccggc atttgatgtg cgcagtgaac
     1681 aggcggcaca ggtggcgatt gcgcggatga accgggccat tgatgaggta ctgagacgat
     1741 gacggaggcg gatttgtatc ctcatctggc gcatcttgcc ggcgggcagg tgtacccgta
     1801 tgtggtcccc ctgctggatg gcaggccgtc ggtggcgctt ccgtgggtgg ttttcagcct
     1861 gatttcatcg gtgtcggcgg acgtgatggg cgggcaggcg gagtcctcag tgtcggtgca
     1921 gatagacgtt tatgccggga ctgtgacgca ggcgcgtcag atacgtcagg acgcccgtga
     1981 agccataatg ctgctggccc cgggatccgt cagtgaaatg caggactata ttccggaaaa
     2041 ccgctgttac cgtgcaaccc tggagtttca ggtcacggtg tgactttttc ttttttctac
     2101 aaaacccata ccccgccgcg tgcgg
//
LOCUS       DAZGCS010000290         2119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGCS010000290 DAZGCS010000000
VERSION     DAZGCS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.295"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..462)
                     /locus_tag="ACHK01_005243"
     CDS             complement(<1..462)
                     /locus_tag="ACHK01_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO8099996.1"
                     /translation="MKIISTVIQTPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFED
                     MNDSSALYKKYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVP
                     GIPLSDIDTADIPDNLESLYLQLICKLNELSIIHYDLNTGNMLYDKESNSLF"
     gene            complement(638..1335)
                     /locus_tag="ACHK01_005244"
     CDS             complement(join(638..1086,1086..1335))
                     /locus_tag="ACHK01_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO8099997.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVLGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELH
                     DKDIGHYLNIKHYQ"
     gene            complement(1596..1865)
                     /locus_tag="ACHK01_005245"
     CDS             complement(1596..1865)
                     /locus_tag="ACHK01_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8099998.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(1867..>2119)
                     /locus_tag="ACHK01_005246"
     CDS             complement(1867..>2119)
                     /locus_tag="ACHK01_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8099999.1"
                     /translation="KGDPGETQIRFRLGPGNIIETNSHGWFPDTDGALITGLTFLDPK
                     DTTRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          558 a          552 c          441 g          568 t
ORIGIN      
        1 aaataaacta ttactttctt tatcatacaa catattacct gtattaagat cgtaatggat
       61 tatactcaac tcattcaatt tacatatcaa ctgcagataa aggctctcta gattatcagg
      121 aatatcagct gtatcaatat cactaagggg aatccctggc acgcgtagca ttcggagata
      181 cacatcaccg ccatactgta taacaactga tgcgtcatcg ccataaaaag tattaaaaag
      241 ggcagattct tgcctagcca tttccagaac ttcattgtgc tggttaccaa caagatcata
      301 ctttttatat aaagcagacg aatcattcat atcttcaaag atttctgctg ttgaaccttg
      361 ccctattaac ttaccgagaa taggctccgt tactacgcca gtatgagaat tattattctc
      421 aaatgggaaa ggtgtttgaa taacagtgga tattattttc atgacatgcc cttgttatga
      481 aatttaaaga ttacaagata tttttacata aaataatatt acgcgctacc acctcagaaa
      541 ccacgtaagt acatatgcta ttacatagat aaccgccatg atcagggaag ccccatacaa
      601 gcacagtgta gagaggcctt taggtaatga ctccaactta ctgatagtgt tttatgttca
      661 gataatgccc gatgtccttg tcatgcagct ccaccgattt tgagaacgac agtgacttcc
      721 gtcccggcct tgccaggtgt tgtctcagat tcagattatg tcgctcaatg cgctgagtgt
      781 aacgcttgct gataacgtgc agctttccct tcaggcgtga ttcatacagc ggccagccat
      841 ccgtcatcca taccacgacc tcaaaggccg acagcaggct cagaagacgc tccagtgtgg
      901 ccagagtgcg ttcaccgagg acgtgcgcca caaccgtcct ccgtatcctg tcatacgcgt
      961 aaaacagcca gcgctgacgt gatttagcac cgacgtagcc ccactgttcg tccatttcag
     1021 cgcagacaat cacatcactg cccggttgta tgcgcgaggt taccgactgc ggcctgagtt
     1081 ttttaagtga cgtaaaaccg tgttgaggcc aacgcccata atgcgtgcac tggcgcgaca
     1141 tccgacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggct gagaggcggt
     1201 gtaagtgaac tgtagttgcc atgttttacg gcaaggagag cagagatagc gctgatgtcc
     1261 ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg gagcaggaag gacatctgat
     1321 ggaaatggaa gccacgcaag caccttaaaa tcaccatcat acactaaatc agtaagttgg
     1381 cagcattacc gagattgttt tgtcggaact gatacgctgg cgctaatagg tagtagggtt
     1441 cttatgctaa acatacaacc tctttcttaa ttctcgaacc tgtttaggat tctgtgtaaa
     1501 ttcaaaataa acctgtctga acattcctaa caaacatcca ccggacatga caacaaaaaa
     1561 ccggagccgg actccggttt ttgtgaagct gtcggctatt tcattccgcc aatattttcc
     1621 cacctcccgt cagcacgcag gatttgcagc ggtcttacca cgcactgtat ctgcttttta
     1681 tccgcatcca gtatcaccac ctgcgtgatt accctgtcct gctccgggat aatgccattc
     1741 tcatcggact ccaggatgtc tgccggcccc agtcgcagct gtgctgtaag taactccccg
     1801 tgttcacggt catcatgctt tccgcaacca cacagacgct gcataagttt ttttagtata
     1861 ttcatgtcat tctcctgttc tgcctgtatc actgcccact tcatccagcc ccttgacatc
     1921 ctgccacggc ccgtcaccaa acctgacctg caaatgctga aaaaaaccct gaacccgtgt
     1981 ggtatctttg gggtcaagaa aggtcagtcc ggtgatgagt gcgccatctg tatccgggaa
     2041 ccagccatgg ctgtttgtct caataatgtt tcccggcccc agacggaacc gtatttgcgt
     2101 ctcccccggg tcgcccttc
//
LOCUS       DAZGCS010000291         2116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGCS010000291 DAZGCS010000000
VERSION     DAZGCS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.296"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2098)
                     /locus_tag="ACHK01_005247"
     CDS             complement(<1..2098)
                     /locus_tag="ACHK01_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100000.1"
                     /translation="MLQIVGALLLLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGG"
BASE COUNT          508 a          578 c          534 g          496 t
ORIGIN      
        1 tcccgcccgc cactgacccg acagcaccac ctgccacacc ggaagatgcc acggcaagat
       61 tcctgatgaa ctgcgcaccg gagatccgtc cccgcaacat tttgatcatg tcagggccgg
      121 ttgtgacagc taccagtgcc agagatgtca ccagcgtacc cttcatcact ttgttcaggg
      181 cactgatatt tcctgcaccg gtccccttct gaagcgcctg ccggacagtc gggctggccg
      241 tcgaaaaatc aatatgcttc agcataccct gaacaacact gagccggtga agttgctggg
      301 ttgtgacgta gacagccagt gtgcgggtga aggtttttcc tgcctggaca gcagctgtct
      361 gcagggcagc atcgcgatcg ccggtgctga gccagaagac cgatgccgtc agggcaaaac
      421 tgatccctcc ggccgccaga ctgacaaccg acccctcggc aatatcataa gtgaccgatt
      481 cgatggtccc gaaccgggtg atattacggg cctgggtata agtcagatgt ccccgacgaa
      541 tcagccggga cgcttctgcg ggatcggtta caccgggtac tttaccttcg cggatcttat
      601 tcttcatggt ttccacagcg ccggcatact gatcacgggg gacttccagt tgcatgggac
      661 catgatttcc catgtaacga tactgtccgt tttgtccgtc gaatgccgca ccgacgctac
      721 gggctgcagt tgaacaatat ttcgtctgga tttctgttcc gctgaccagt ctgtctgctc
      781 catttctggc attattatca ccgaggatcc gtgcgttttt cagacggact ctgtcgacaa
      841 tatggtttcc acgttcagcg gcaaacccat gtccctgccc tccggaagca aacattttct
      901 catttgtact ccaggtttcc ggcagtttca tgagaatcag accattcgcc gttgcatcgg
      961 aaataaaacc ggtgtaagaa cgttcataaa tgtcctgaca cagtgtttct gtttcgcgac
     1021 aaagagagga gcagtaccat tccggatccg gcttatgatt ttccggggca tgatgtactt
     1081 ctgtcagccc gcaggaggca cagatacgaa gctcatccac gtggtggaat gatacaaaac
     1141 ggcggccttc tgaaaagggg taccagtcac cgccgtccag gcaacgatcg agaaaatcgt
     1201 cattcgcttc cggtgataaa ttcaggcggt tccggaagag ggttttcagt tcatcagggg
     1261 taaatgacag gctctctctc agcttttcgt tgaaacccgc catgtttctt tcataccagg
     1321 cattgtccag tagccagttt ccgtctgcca gttcaaattc cctcagttct ccccgggaaa
     1381 tccgttgtga aatgagtgtc agccagatgc gctccgtcag cgtttcagga aggctgtaat
     1441 cgttttgcaa actgctcagc cacgatttac tgttgagcac cggttgttct tccagtgtct
     1501 ggcggatggc actggtcagt cttttcagcg cctggatctg gctggccgcc catagcagcg
     1561 tcactaatgc agccagccag tacaacaacc cgccccacca catcagtgag ggtgaaatat
     1621 cgaactgcaa tgcgatcaat ggtgcgtatg cagaacacgc cataaggaaa aaagcagtat
     1681 aaaaacagca gtaatacccg ttaatttcct tgctgatatc ttcaggtgtg agcccgttct
     1741 gctgaatgct tcttatctgc acgggaagag ccagcagacc agcggcgaga gcggtggtca
     1801 gaataaattt tacagagtca aaagtatgag cctccagtgc gatatgttta ccatcaagtc
     1861 cccacatgaa gctgatgatc attccggcaa tcgttagaaa tacgcctgcc agccagcgaa
     1921 tatggaataa ccgggttatg cgttcagtga tatagccagc cagtaaagcc gggccgaaca
     1981 gacacagcaa tacgaatcct gccagcgcag acgccgtgct ggttaatgct ctgaacaaaa
     2041 ggcgaagaat ggcaaatcct gcgatcagca ggagcagcgc gccgactatc tgtaacatag
     2101 tcactccgtg atgaat
//
LOCUS       DAZGCS010000292         2041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGCS010000292 DAZGCS010000000
VERSION     DAZGCS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.297"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..641)
                     /locus_tag="ACHK01_005248"
     CDS             complement(<1..641)
                     /locus_tag="ACHK01_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8100001.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART"
     gene            910..1026
                     /locus_tag="ACHK01_005249"
                     /pseudo
     CDS             910..1026
                     /locus_tag="ACHK01_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1131..1207)
                     /locus_tag="ACHK01_005250"
     tRNA            complement(1131..1207)
                     /locus_tag="ACHK01_005250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Arg,seq:tct)
     gene            complement(1221..1297)
                     /locus_tag="ACHK01_005251"
     tRNA            complement(1221..1297)
                     /locus_tag="ACHK01_005251"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Arg,seq:tcg)
     gene            complement(1305..1380)
                     /locus_tag="ACHK01_005252"
     tRNA            complement(1305..1380)
                     /locus_tag="ACHK01_005252"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Ile,seq:cat)
     gene            complement(1409..>2041)
                     /locus_tag="ACHK01_005253"
     CDS             complement(1409..>2041)
                     /locus_tag="ACHK01_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100002.1"
                     /translation="GSVVLATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKLTRT
                     HLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGC
                     LSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRV
                     AVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          523 a          543 c          491 g          484 t
ORIGIN      
        1 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgtgc cgaatcctgg
       61 ctggcccccg tgtccgcact gaatgtcccc tcagcgccct gggtgaatgc agaaccacca
      121 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
      181 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      241 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcagccgg aatgatgtcg
      301 ttatacgtac aactctctcc acccggagtt accgtgctgc ggcgcgccag ctgtttaatg
      361 cgcggatccg gagcatcgta agaatccggt aacggaagcc cttcaccgta ggccatgccg
      421 ttggactgtc cggcaagcac aaccacgtag aaccagtccg gctcagatga agggccgacc
      481 tgtggatctc cttcaatagc caccgcctgc atcagtgtgt acggcgtaat ggcaaccggt
      541 ccgccgtatg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      601 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      661 aatattgtga gtaaaatgag gagcgggctg aagtccggaa gttacaggac aatggcagaa
      721 gagagacaac agcccgcaat acgaaaaagg ccgcgctatt gcgcagagtg attactgtcg
      781 gatattattc gccagctgaa atattacttc acgttttgtt gtttattcct tgccgcccgc
      841 gtctccctgc gcgggctttt tttgtccata agaaagcccc tccggagagg ggctggagag
      901 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      961 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
     1021 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
     1081 tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg
     1141 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     1201 acaacgcagg atacagataa tggaccgcct tcggggaccc gaactccgcg caaccagctt
     1261 cgaaagctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg cccttgctgg
     1321 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1381 gggtcaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg gatacatatc
     1441 tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac cagcttgaat
     1501 aaccgcattt ttctgctaca gcctgtccat atccatgccg ggaacataac atattcacag
     1561 caacacgcat ccgctcttcc agcaacaagc gactgaacat gtgcccttca tttttcagtt
     1621 ttatctttaa caaactctca ctcatatgca ggcgtaacgc aatcgcacca agcgtccagc
     1681 ttgctgatat atctgtctga attatcgccc tgactttggc acttatactg gataaacatc
     1741 cacttaaaaa taataatatc cgttcatctg attcaaacag cgacaggcat gccatcataa
     1801 gaaacatatc cgtggtatct ccggaaaatc tctggctggt aattaaagcc tcagccaacg
     1861 cagggttgtt gggttccagt gacagataaa gcggaacgtc agtcagatga gttcttgtca
     1921 gcttatgctg aatttccaga tattgactta ctatggaatg gtttatatcg aaaattttaa
     1981 ctttgccata atgcataagg aaaagcgccc tgatgcattt ggtggccaga acgactgagc
     2041 c
//
LOCUS       DAZGCS010000293         2020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGCS010000293 DAZGCS010000000
VERSION     DAZGCS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.298"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHK01_005254"
     CDS             <1..99
                     /locus_tag="ACHK01_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO8100003.1"
                     /translation="RPVILEAQDVLITYRGKLPGIITGSLKTPPLA"
     gene            150..683
                     /locus_tag="ACHK01_005255"
     CDS             150..683
                     /locus_tag="ACHK01_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8100004.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            954..1523
                     /locus_tag="ACHK01_005256"
     CDS             954..1523
                     /locus_tag="ACHK01_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO8100005.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            1523..1669
                     /locus_tag="ACHK01_005257"
     CDS             1523..1669
                     /locus_tag="ACHK01_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100006.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFSLMLVALFLSMAWMALTSA
                     GLVG"
     gene            1681..1956
                     /locus_tag="ACHK01_005258"
                     /pseudo
     CDS             1681..1956
                     /locus_tag="ACHK01_005258"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1907..>2020
                     /locus_tag="ACHK01_005259"
     CDS             1907..>2020
                     /locus_tag="ACHK01_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO8100007.1"
                     /translation="MKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQP"
BASE COUNT          539 a          448 c          544 g          489 t
ORIGIN      
        1 cgccccgtaa ttttagaggc gcaggatgtg ttgataacct acagaggaaa actaccggga
       61 ataattactg gttctctgaa gactccaccg ctggcctgaa gacttaacat atccagggat
      121 ttgaaatcga taaaccctga taaatatcca tgaacgcaaa aatcagatac ggcctgtcgg
      181 ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc gaccagtttc
      241 ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt atctggacca
      301 tctgccgcgg tgccatcttg gtggatggta aacctgtcgt tccgggcatg aagttgtcga
      361 aggaaaaatg cgaccgggtt aacgccattg agcgtgataa ggcgctggca tgggtggaga
      421 aaaacatcaa agtgccgctg accgaacccc agaaagcggg gatcgcgtca ttctgtccgt
      481 acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt aatgcaggtg
      541 atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa ggacggtggc agagactgcc
      601 gtattcgttc aaacaactgt tacggtcagg tatcccgtcg tgaccaggag agcgcgctgg
      661 cgtgctgggg aatcgacaga taagaagaat attttgctga aaaatgaggt ttgcttacat
      721 ggatggataa cacgaaatcc tgcaaattgg caaaatgtaa gtgaataaag tcaaaacagt
      781 tgtttaacac tcaggcaccg taatgatgcc tttgtcattt ctgcgcatct cacgcgcatc
      841 tcacaacaca gaacctttca ggatgaccct tgaggatacc ggtttggctg tcggtgcctt
      901 tctgtgggct ggattcctgt gagacaaggt tcatcactaa aaggaaataa ccgatgaata
      961 tgatggccgt gccgtttcac ggcaactctc tttatgtagt taaccataat ggcgaaccat
     1021 acgttcccat gaaacctgtc gttgcgggga tggggctggc ctggcaatca cagttggcta
     1081 agttaagaca gcgttttgcg tcaactataa cggaaatcgt tatggttgct gaggatggga
     1141 aacaacgcaa tatggtgtcc atgccacttc gaaaacttgc cggctggcta caaaccatta
     1201 atcccaacaa agtaaaaccc gaaatccgcg ataaggtcat ccggtatcag gaagagtgcg
     1261 acgatgttct ttacgagtac tggacgaagg gttttgtcgt taatccccgt aaaatgagcg
     1321 tgatggaaga actcaaccag gcttgtgctg acatgaaacg ggataaaaac attgccagtg
     1381 tgtttgctac cgggctgaat gagtggaaac aggttaaagc cgcgcatgta tcaaaaatcc
     1441 gtacgctggt aaatgaagcg aatatgctga ttgattttgt cctggctgat acaggcaaag
     1501 ggaaaataac aaaggcggat tgatggggtg gctaatgata tcagataaac tcataacgct
     1561 ggtgaagagc ctctgtgtac ttgtcggcat ttcattttca ctaatgctgg ttgctctttt
     1621 tctttccatg gcctggatgg cgttgacttc ggcagggctg gtggggtgag cataaaccga
     1681 atgctttccg cgtttaccgt tattctgctg gtggtctgtg gtgcgcttag tctggggctg
     1741 aatcattacc gcgataacgc cataacctac aaagagcagc gcgataaaaa agtcagtgag
     1801 ctggagctgg caaatgcaac cattactgat atgcagcagc gccagcgtga tgttgctgca
     1861 cttgatgcca gatactcgag ggaattagct gatgcgagag ctgaaaatga aactctgcgt
     1921 gctgatgttg ccgctggtcg taagcgcctg cgcatcaacg ccaactgtcc aggctccttg
     1981 cgtaaagccc ccatcacctc cggcgtggat aatgcaaccg
//
LOCUS       DAZGCS010000294         2013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGCS010000294 DAZGCS010000000
VERSION     DAZGCS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.299"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..137
                     /locus_tag="ACHK01_005260"
                     /pseudo
     CDS             1..137
                     /locus_tag="ACHK01_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(332..499)
                     /locus_tag="ACHK01_005261"
     CDS             complement(332..499)
                     /locus_tag="ACHK01_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO8100008.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(496..927)
                     /locus_tag="ACHK01_005262"
     CDS             complement(496..927)
                     /locus_tag="ACHK01_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO8100009.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENYL"
     gene            complement(1097..1173)
                     /locus_tag="ACHK01_005263"
     tRNA            complement(1097..1173)
                     /locus_tag="ACHK01_005263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1137..1139),aa:Arg,seq:tct)
     gene            complement(1274..1349)
                     /locus_tag="ACHK01_005264"
     tRNA            complement(1274..1349)
                     /locus_tag="ACHK01_005264"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1314..1316),aa:Ile,seq:cat)
     gene            complement(1378..>2013)
                     /locus_tag="ACHK01_005265"
     CDS             complement(1378..>2013)
                     /locus_tag="ACHK01_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100010.1"
                     /translation="PGSVVLATKCIRELFLMHYGKVKIVDISESVVSQYLESQHKLTR
                     TRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSG
                     CLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMR
                     VAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          552 a          545 c          443 g          473 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggctggt cgtaaaaaaa aggccgcgcc
      121 atgcgcagcc gaaaataaag ggataacgat gatagtttga gaaaaacaga aataacactt
      181 ttgtggcaaa gcatggtgcc gggtgcctcc cggtgaattc agtatcagca cctgaatccg
      241 cgattacccc atattccttc ttgctgattg ccccaccgca cagggggatt caccatgcag
      301 aagtgttttt aataaacagc aaacaaaaaa atcaagcatt atgcaggctg tttcttttta
      361 tcaccggcca cagcaatacc ataatgccgc agaccagcac cccatccgcc agcaccgaca
      421 tgattctgct ggtgaaatcc accatcacca ccagaaacag caggagtgca gccacagtca
      481 ggcgcagttt taccgtcaca ggtaattctc cagacgaaga cccagaacac cggcaatctc
      541 ttccagcacc ttgcgctctt ccggctcaat ttcgccgtct gcctccgcaa tggccaccgc
      601 cacatccagc acatcttccg cttcacgcgt atcgtgtttc acatcctcaa tctcgcgtaa
      661 cgcggcacga cgaccaattt taaaattggt atccagctga ccgataatag ttgcgctaat
      721 cgcattaatt tccgaggtaa acgcagacaa cgcaggctga ttacgtaaga cctgttcgat
      781 cttcgctttc tcggatgcct cgcattcacc atctgcatag gccaccaggt atgcagcgtt
      841 aatcaccgcc tgtgccagat cgcgtttctc aaacttttta atttccgctg ccgctctgcg
      901 ggttttcttt ttgaagattc caaacatcgt gacgttcctt tgggtgggtg agccaacgcc
      961 cgggagcgat ctgcccacag agaaagtcac actgaccact ccgtaagctc acccccgaaa
     1021 ggctctgtgg ttgatatgcg ccgggcgtgg cgcagataca aaaaaggccc gccgaagcga
     1081 gcctggaaaa taagtgtggc gcgttgtagt ggagtcgaac cactgaccga ttgcttagaa
     1141 ggcaattgct ctgtccggct gagctaacaa cgcagaatac cgataatgga ccgccatcgg
     1201 ggacccggcc ccgcaccaac aaccctgtta tggtgtcgtc tgctcttcct gataagctaa
     1261 tggcggtttg tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc
     1321 gctcgctctc accactgagc taaagggccg agccaaaaaa taataatcag atgaaaatca
     1381 ataatcaagc ccttgcctgg atacatatct gtctggcggg aagccataat agcggtgaaa
     1441 tacagaaata aagtaggacc ggcttgaata accacatttt tctgctacag cctgtccata
     1501 tccataccgg gaacataaca tatttacagc aacacgcatc cgctcttcca gcaacaagcg
     1561 actgaacatg cccccttcat ttttcagttt tgtctttaac aaactctcac tcatatgcaa
     1621 ctgtagagca atcgcaccaa gcgtccagct tgctgatata tctgtctgaa ttatcgccct
     1681 gactttggca cttatgctgg ataaacatcc acttaaaaac aatgacatcc gttcatctgt
     1741 ttcaaacaga gacaggcagg ccatcataag aaacatatcc gtgacctctc cggaaaatcc
     1801 ctggctggta attaaagccg cagccaacgc aggattgttg ggttccagca acaggtaaag
     1861 cggaatgtca gtcagacgag tcctcgtcag cttatgctga ctttccagat attgacttac
     1921 gacggattcg cttatatcga caattttaac tttgccataa tgcataagga aaagctccct
     1981 gatgcatttg gtggccagaa cgactgagcc tgg
//
LOCUS       DAZGCS010000295         2005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGCS010000295 DAZGCS010000000
VERSION     DAZGCS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.300"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..548)
                     /locus_tag="ACHK01_005266"
     CDS             complement(<1..548)
                     /locus_tag="ACHK01_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO8100011.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDEL"
     gene            complement(564..1298)
                     /locus_tag="ACHK01_005267"
                     /pseudo
     CDS             complement(564..1298)
                     /locus_tag="ACHK01_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(1295..1626)
                     /gene="sctI"
                     /locus_tag="ACHK01_005268"
                     /pseudo
     CDS             complement(1295..1626)
                     /gene="sctI"
                     /locus_tag="ACHK01_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1646..1886)
                     /locus_tag="ACHK01_005269"
                     /pseudo
     CDS             complement(1646..1886)
                     /locus_tag="ACHK01_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
BASE COUNT          619 a          384 c          342 g          660 t
ORIGIN      
        1 agctcatctc tatttttttt cgcataaaat gtaattctct ctaaaagagt tgatgcaagt
       61 ggtaacggca attgtatgtg atcaacaaaa tcaggaaata ataaattaaa tcgttgctgc
      121 attgccaatg gttgttgcct tatatagggt gacaacgttg aaaaaccaca tgtgatgata
      181 ttgtgatagc tagcgatgcc gggtttgtat cgtgcctttt catttatttt caccggtata
      241 gctgataaat aatcccttag cacagcagga accttataaa aaaatccgcg ttctgcaaac
      301 ctttctcgat aaaagtgata accactgaat aaacagaaca atggaaataa attccaattg
      361 tttatgtagt aaataagtga actattcaga ttaaaatgtt caactgaaag attgtactgc
      421 agaacaatca tttcattggt aatagatcta agcaccggat tgttaatagg tgtattattc
      481 aagcttactc gttgaggatg aatataacta attggatcat atataatctt tcttagggca
      541 agattcataa cgctctcata tgtctactta attttcaacc tgactaaatt agttagaatg
      601 gccctatact tccataacag ccagcaagtc gctacggata ttaatgcaag taagatagaa
      661 accggcatag ccttatcata agcaaaaaca ggttcgctaa tttcatatgt tggtgcttgc
      721 tcaataatgt ctcttcgttt tgacaataca acagaaatat tttcatattg tacgcttgca
      781 gagctattaa caataaatct cttgatatca tttattttta tttctgggtt gatatctttt
      841 tcatatactg caagtacaga aatatgaatt ggtaaagcag ttttaccact atcgccatta
      901 tcaacatcgt aactaacatg tactctcgaa gaaataatgc catccataat tttgagagat
      961 tgctctaacc gctgctcaat agcagaatat agccttgctt tttccgctcg tggagatgaa
     1021 acgagtgcat ctgcagggaa catctgcgat atttgaatat caggcttacc cggtagattg
     1081 tagattttta gccaatccac cgcagaagca aaatccgttg gttcgacaaa tattgaaaat
     1141 cctgttttgc cttaatcctt cttttcagca ttaatattat gtctttgtaa aacagcaagg
     1201 acatcattag cttgctgttg atcaagatgg ttcaataatt cctgctgctt gcagccgcac
     1261 aacagcagga taaacaataa taatgatata tatttcatga gcgggtcaac gtctcaactg
     1321 cagcgacaat ctttcttgct aatgttccac caatgaaacc tcgacattat aattaagcac
     1381 atgatcctgc aagactaata atttttcagg agttaacccc tctttatcag accttaatgc
     1441 ttgtaacgca ttttgctgcc aattttcagt ttgtacagca tagttggcaa aagcggagat
     1501 cattttatca tccgggttgt caagattgag aacccggtca tttaaatcaa tcactttcgc
     1561 ttgctgtatt tcatgagcag ttgattgctc agctctatca ataggtggca tattgcttac
     1621 tgacataata aaatccttat ccagtttagc ggaagttttg tatgattgct gaatcaagat
     1681 ctttatacgc ttttacaacg ttagattgcg catttcgata taatgtatac tcagctaatg
     1741 cactttgata ttcggcaaga aatttcgtgt cggagggatt ggttggctaa atcctccaac
     1801 gctttttcaa cttgctggtt cagatcatca acgccctgat caaattgatt gctgatatcc
     1861 ataatataac cattccaatc tgccataagt cctcctcaat tattcctgaa atgtccagtt
     1921 gctaccttga gacttctcga atcggttatc tccgtagcta aatgaactat tatttgtgtc
     1981 ctgatttttc aaattcacat taaat
//
LOCUS       DAZGCS010000296         1997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGCS010000296 DAZGCS010000000
VERSION     DAZGCS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.301"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..421)
                     /locus_tag="ACHK01_005270"
     CDS             complement(56..421)
                     /locus_tag="ACHK01_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8100012.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELEHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGSGRADDITGL"
     gene            463..648
                     /locus_tag="ACHK01_005271"
     CDS             463..648
                     /locus_tag="ACHK01_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8100013.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQLNIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(778..918)
                     /locus_tag="ACHK01_005272"
     CDS             complement(778..918)
                     /locus_tag="ACHK01_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100014.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1275..1499)
                     /locus_tag="ACHK01_005273"
     CDS             complement(1275..1499)
                     /locus_tag="ACHK01_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100015.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1564..1770)
                     /locus_tag="ACHK01_005274"
     CDS             complement(1564..1770)
                     /locus_tag="ACHK01_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8100016.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
BASE COUNT          553 a          539 c          441 g          464 t
ORIGIN      
        1 ggcggtgtcc aaagcgatct ttttttacgc cggatgatac cccccccggg tcgggttaca
       61 gtccggtgat gtcgtccgct ctgccactac ctccggacac ctccggcagc gtcgggtctg
      121 gcataccact cgccgcttca cgagcagact tttgtcgatg gcattcggta cagagcgtcc
      181 agagattcgt ctcctcatta ccaccaccga actgaagtgc aatgcggtgc tcaagttcac
      241 tgtcacaaag gtcaaccaca cgcccacaga gacagcactg tccggcatcc ctcagccaga
      301 tacgacgctt gagggaaacc cgggcactac cactgacccg acgctgttca cccttcagga
      361 cattcacccg ccgggtgttc agagttttga ttctgcccgg taacgtacga agcacagcca
      421 tgtaaaatcc tctccatata gcttgtcacc agaggaaaga aaatgtcatc gaaaaaccgg
      481 acccgcagaa ccacaatccg caatatccgt ttccccaatc acatgattga acagctcaat
      541 atcgcccttg aacataaagg ttccggtaac ttttcagcgt gggttattga agcctgcaga
      601 agaaggctgt caacagagcg ttcgggtatg aattacataa ttaagtaaca tggtgttcac
      661 agaacacaca gttaccggac acatcagttt tccattcgtc ccccggcagt acaggcttcc
      721 cgtctgacgg gatagcctga aaaaacacag aaaattattt gttataatta atataactta
      781 ctcaaaaaaa agcgacgaga aaatcagcat caacgagcaa taagcgccaa tacgtgataa
      841 caaatggcag ccatatttat ctgcagtata agcaatggac aggataacca caccagaaac
      901 cgtcagcata aaatccattt gaacttcccc ggacaaaatc gactcatcta aagatttaca
      961 gctcttttta ttatcaatat gttaaaagta aaataaacag atgttcaata atacgaatac
     1021 aaaaacgtgc tgaaaatcaa tgaatccatt tctgtgttat caattaatag tgataaacat
     1081 ccggcttctt ccaccatcgc accggaccag cgaccatgag gggacaacgc cgcgctccgt
     1141 taacgcggta aaccccggtg tgtatcgttt ttgattatcc ccgcacactc gcgcagagga
     1201 ttctcccggt cgggctgcgg tctctgttaa tgcaggaata cggcgacaat accgcgcatg
     1261 aataataagg tcgctcaaca cactggctgt aatgcagcgg ataccatgcg gcatttagcg
     1321 gcattcatcg tacactcaac ggttagctct tcattcgtgg cattcacctg aaaggtccgg
     1381 gagtgtaatt gcgtacattt accactgaac gaaccttcaa caagaacacg accacgctgc
     1441 aaaatacgga acggaattgt tccctgaaaa ggctttacgg ttaccaataa tttcttcatg
     1501 cattctccga ataacaaaaa tactagttaa tacactgagt gcggatatat tcctgaagca
     1561 ttctcaatgc tgcctggtcg ctgatgattc cgtctctgat accgagaacg tttcgtccag
     1621 caaccggaga gagttcgacg gcggcatcat tgcccacgcc ggaggtgccg gtggcttcac
     1681 gcacggtacc ggggcaggtg gcgttgatcc gcaggcgctt acgaccagcg gcaacatcag
     1741 cacgcagagt ttcattttca gctctcgcat cggctaattc cctcgagtat tttgcatcga
     1801 gcgcagcaac atcgcgctgg cgcacctgca tatcagtaat ggttgcgttt gccagatcca
     1861 gctcactggc ttttttatcg cgctgcgctt tgtaggtgat ggcgttatcg cggtaatgat
     1921 tcagccccag actaagcgca ccacagacca ccagcagaat aacggtaaac gcggaaagca
     1981 ttcggtttat gctcacc
//
LOCUS       DAZGCS010000297         1994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGCS010000297 DAZGCS010000000
VERSION     DAZGCS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.302"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..>1994)
                     /locus_tag="ACHK01_005275"
     CDS             complement(33..>1994)
                     /locus_tag="ACHK01_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8100017.1"
                     /translation="IEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLIS
                     LVDGSGNPISVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQLAPGNYRLTVRAVN
                     AWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSETRIT
                     DIRQVETTARYLGTALYWIAASINIKPDHDYYFYIRSVNTVGKSAFVEAVGQPSDDAS
                     GYLDFFKGQITESHLGKELLEKVDLTEDNASRLEEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDAEEGKLSQFLVAANRITFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGA
                     KHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAW
                     LVNNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          411 a          599 c          497 g          487 t
ORIGIN      
        1 catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc tgcggtggct
       61 ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca gttattcact
      121 agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg catatcaata
      181 acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc agcaccgttt
      241 ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc atgcttagcg
      301 ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc gtcataaacc
      361 ctgacggtaa tggtccctga tggccaccgc tccggtgccc gggagtcccg cggaaaagcc
      421 ttacccactg ttttgactat atcgccttca atctggttgg ctgacagttt ccccttaatc
      481 tgacagttct cattaattgt gacattgttg agcgtcccgg cgttcgcatt cacactgcca
      541 ctgatatctg catttttagc ggtcagcttt ccgtccggtg tcagggaaaa tgccggagga
      601 ttaccgccgc tggtaatggt gggagccgtc aggcgcttca ggaacacgtc gttcatgaat
      661 atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg gtcaataaat
      721 gtgatacgat tggcggcaac cagaaactgg ctcagtttgc cttcctccgc gtcctccatg
      781 ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctcaat tttgacgccc
      841 cacatggcat tccacttatc gttggcgtcc ttccactctt tcgaaaactc ctccagtctg
      901 ctggcgttat cctccgtcag gtcgactttt tccagcagct ccttgccgag atgggattcg
      961 gttatctggc ctttgaaaaa atccagatag ccggatgcat catcactcgg ctggccaaca
     1021 gcctccacaa atgccgattt gccaacggtg ttcacactgc ggatgtaaaa ataataatca
     1081 tggtccggtt tgatattgat actggcggct atccagtaca gcgccgtgcc aagatagcgg
     1141 gctgtggttt caacctgcct gatatcggta atccgcgttt ccgagaacca gaactcaaac
     1201 tgtaccgtcg ggtcataaac cgcaagatgc ggcgtggcgg ttatctgaaa atagcccggc
     1261 gtcagctcaa tctgtgacgg cgctgccggt gcggcaatcc tgaacgatac cgatgccgga
     1321 tcgccctgct gcccccacgc atttactgcc cggactgtca gcctgtagtt ccccggcgcc
     1381 agttgtgtga agcggtatgt ggtttccgcc gtccgggctg tgctgaccag ccgctcactg
     1441 ccgtcatccg cggccacggt caggcgaagc atgaaactca cgcccttcac caccttcggc
     1501 gtatcccagc gggccagtac ctgatactcc ccgctgtctg cggtgacttc ggcagtcagg
     1561 tgctgcactg ctggcggcgt gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc
     1621 gccccgttat ccacgatggc ttctttttcc ggtacatgct gcacggcggt gatagcatac
     1681 gtgccgtcat cgttctcacg gatactcaca cagcggaaca ggcgctggcg caacgtcggc
     1741 aacttcagcc cccacacgct gtattcagca acaccgtcag gaacacggct cactttcacc
     1801 ttcacgccgt cggtgacgga ctgaacctcc acgctgatcg gattgccact tccgtcaacc
     1861 aggcttatca gcgtggtacc ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc
     1921 cgggtctggc tgtttaccgc cagcacgcgc ccgccggtgc tgataccggc atagtcatca
     1981 tcacagattt caat
//
LOCUS       DAZGCS010000298         1989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGCS010000298 DAZGCS010000000
VERSION     DAZGCS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.303"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..833)
                     /locus_tag="ACHK01_005276"
     CDS             complement(<1..833)
                     /locus_tag="ACHK01_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO8100018.1"
                     /translation="MATLRELIIKTSANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VT"
     gene            complement(885..1094)
                     /locus_tag="ACHK01_005277"
     CDS             complement(885..1094)
                     /locus_tag="ACHK01_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO8100019.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(1190..1564)
                     /locus_tag="ACHK01_005278"
     CDS             complement(1190..1564)
                     /locus_tag="ACHK01_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8100020.1"
                     /translation="MAKDLKTLALARLSGFRHKMVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(1570..>1989)
                     /locus_tag="ACHK01_005279"
     CDS             complement(1570..>1989)
                     /locus_tag="ACHK01_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO8100021.1"
                     /translation="KVQFPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSY
                     VVPLAFVKNPEKTLTVNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKAN
                     AQSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
BASE COUNT          424 a          660 c          464 g          441 t
ORIGIN      
        1 gtcaccccgc tggcctcatt cgcaccaccc acccaggcca taaaggcgtt ttccaccttt
       61 gtgatcccgt cagagaccgt ttctggcatg gcggcatatt catcacgcaa tgtccccagc
      121 tggctgatta acgcgggcac gactttatcc gccgtcagtt tgccgtcgtc cgccatcgcc
      181 ttcagatctt tacgggccac gcccatgcct gcagccagtg cacgaatgat ccggtcacca
      241 ctttcattga ccgaattaaa ttcctcaccg cgcaacacac cctgtgccag cgcctggctg
      301 aactgggtga tcaccgaacc cgcctcagcc gtactggcac cggagatttt cagccccgtg
      361 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcatcgag
      421 gcagccgaac gggcaaacag ggccgcatta tccgaaaatg ccgtgcctgt ccgctgactg
      481 atatccatca gcactttctg tgatgacgaa aattcatcgg atgactgtga tgcctgtttc
      541 agacgggcat tcacggaact ccactcatcc gccagagaaa tcaggtgtcc ggtggcaaag
      601 gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
      661 ttcagttctg ccagggcacg tcgctgctcc ctggctgccg cagcagcctg acgtccgcca
      721 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
      781 aatgactgtg aatttgccga agttttgata atcagttcac gtaacgtcgc cattcacctt
      841 tctccgggca aaaaaaacct gccacagcag gttttcacca ttatttatga cattgctgca
      901 aggctcagcg cgtcttccag cgccgcaaac ggatccacct ccggcttatc ctcatcctcg
      961 ccccagcaga gcatggcgtc cttcagtgca acattcatcc cctgtgcccc gaaaaccgct
     1021 ttcacgatct gtgcattacg gatatccccg cgctcatcac ccagcgggga taccctgtcg
     1081 aactccatcc acatcatcgc ctcgctcgca ctcaggctgt gccgcagttc ggataaggtg
     1141 cgccccagac ggagcgcaag tcgcatcaga aagcgaattt ccgggcgggc tacttttttc
     1201 tggccgactc tgcatcagcg atcagttcca gtgcctgacg cagcaaccgg gcatgtaccg
     1261 gaccatagac ggccagcacc tgctcacggt cgtccggagt gaacacccgt tgcaggtcag
     1321 tatcacacag gacatcgcag aacagcgtca catccgcttc caggttacgg cgggttttcg
     1381 ccaccaccga cagggtatcg tcatcctctc catcaccatt gagcacttcc tgccacagat
     1441 accaggcctc tgccgaaggc tcccgcagca ccacgctgac atttctccat tccggcacct
     1501 tcaccatttt atgacggaac cccgacagtc tggccagcgc cagtgttttc agatcttttg
     1561 ccataagcct tatccgcccg caccattaac cgttaccgta cacgcatcag aggtaatgct
     1621 ctgcggctgt tctgcagaat ccgttacctc gcaggtataa gcccccttat cacctgactg
     1681 cgcattggct ttactgaaag tgtcagtagt ctgtccctct accggctgac catccttctt
     1741 ccaggcgtgt ttataaggcg gcgttccccc gttgacactg actgacattg tcagcagcgc
     1801 accggtattc acggtaagtg tcttctccgg atttttcaca aacgccagcg gtaccacata
     1861 ggacaccggt ttacccttca ggcgaagtga gaacgttgca gccaccacgc cgttggtacc
     1921 ggatgaccag gtgtgctgac gcacttccgc caggaactta aagcccttac cggacggaaa
     1981 ctgcacctt
//
LOCUS       DAZGCS010000299         1969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGCS010000299 DAZGCS010000000
VERSION     DAZGCS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.304"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(477..>1969)
                     /gene="nleL"
                     /locus_tag="ACHK01_005280"
     CDS             complement(477..>1969)
                     /gene="nleL"
                     /locus_tag="ACHK01_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO8100022.1"
                     /translation="FKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEK
                     SNTINLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIK
                     KISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMM
                     YMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKV
                     YPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTS
                     TNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNE
                     LKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMP
                     LRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYY
                     YNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          705 a          410 c          261 g          593 t
ORIGIN      
        1 tgaatatcaa ttcataaaat atgattctcc acgatttgcc cagaaataac gaacataatt
       61 ttcgttgtaa ttaaaatcat atttcatcta tagtgaccat tcacagcaaa acaaaatcaa
      121 ttatcacatt caaaaaaaat actgacaaca aagatactct tcagacaaag ttgtcatatg
      181 taaattttcc cttaaaacgt tgctcaaaaa aaatataaaa atcatatgcc ctaatcaaca
      241 tcctcaagca ctacgatata tgcagtatcc tattcatata ttaccaccat atcagacaaa
      301 tcgaatcaca acgagttcta tcgtactctt tcatcttcga cactgtaaat tttaagaaca
      361 ttcggctcat gccactaaac caagacataa taacaacgga gcaataaatg ttcccactga
      421 acatgtgcaa acctaaaaag acagtgttac aataatattc atccatggtc tctaaatcaa
      481 cgccacgcaa caggataata caatgaaaat atttcaggaa aagattcttt tgcatgctcc
      541 gttagcatat cagtcagcac agcggtacaa gtaaaaacat tatttcttcc ttgtagcctg
      601 tctaaaatat cattgtaata ataaactgag ccaaatacct gaggatctgt tttataagct
      661 tgttcaatca atccatttgc atatctccgt agaatctcag gagaatcata ctctgtacca
      721 aacatatcac tcgatgagta tttacagaaa acccccccta aacaaaataa tatttcagcc
      781 tgttttctta gtggcatatc tttcaataca tggctcccca taatcatatc tacatgctga
      841 gcgttcagtt cccaaccatc aagaaaggga ttccagacac aggcaagttt cctttgctga
      901 tcatcagcaa tcattttata atctgattta ttggaattaa gtgcctcaat aaatctctct
      961 ttgagttcat tatcagaaat gagtatatcc agaataccag aaacgaaatt ctgggaaaaa
     1021 gctttatgat aaggaatact gaaaattgga aatgattcag aaaaaagatt gctcaatgaa
     1081 tacccaaatg tattttgaac ttctccatcc ttatataaaa aaaaggacat ccagttagtt
     1141 gatgtattag gagataacat atcaaccata tgactcaaag aacacatcat tgccatacca
     1201 tcatcctgtg atgataacaa aacccagtta taagccaaat cggaatcatc atcccagtca
     1261 ggcttcccag agccatcgca caatccaaaa tcactctgct gtgtgacagg ataaactttt
     1321 tcatttttta aatatacttc atagagttcc ctaaatcgtt cctgcattcc atcacgccgt
     1381 gaaccattca ttacaatttg tataaaggaa ctattataag ataccatcgc acctggatgt
     1441 cgctcaaaat acatccctgc ctgcaataaa ctaccattct ccatatacat cattattgaa
     1501 ttattaaacc aagacattat agtatcaaaa aagtcatcac atataggctc gagccattgc
     1561 tgtatatttg cgttgttcaa ataaggatga atactaaatg aatcagccaa agacatctta
     1621 acatagggcc ggaacgccgc taattcgtca agagatattt ttttgataag ttcttcagcg
     1681 gcccttactt tatcatggtt atacttatca tcagatgtat tcatagtcaa taaaattgat
     1741 ttatttgatt ttgtatagtt atccaacaca gataatatat tttcctgtat gtcagataga
     1801 tctatgtaac accctcctag attaatggta ttactttttt cactaaagag ctcctttaca
     1861 tcacctaaga tagcaccact taaaaccatt ccaggaatta taatagctcc tgttaaatca
     1921 gcctcgttca aaattggagg atattgtata tatgaagatc ctttaaatg
//
LOCUS       DAZGCS010000300         1914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGCS010000300 DAZGCS010000000
VERSION     DAZGCS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.305"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(106..387)
                     /locus_tag="ACHK01_005281"
                     /pseudo
     CDS             complement(106..387)
                     /locus_tag="ACHK01_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(534..1394)
                     /locus_tag="ACHK01_005282"
     CDS             complement(534..1394)
                     /locus_tag="ACHK01_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO8100023.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1577..>1914)
                     /locus_tag="ACHK01_005283"
     CDS             complement(1577..>1914)
                     /locus_tag="ACHK01_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8100024.1"
                     /translation="DMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAE
                     RRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVY
                     KILEDERAS"
BASE COUNT          495 a          512 c          426 g          481 t
ORIGIN      
        1 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
       61 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg accccaaaca ggaaacgcag
      121 ctgaaacggg aagctcaaca cccactgacg catgggttgt tcaggcagta cttcatcaac
      181 cagcaaggcg gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg
      241 cttacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
      301 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
      361 caattccttt ccctgctctg ccataagggg tctgacgctc agtggaacga aaactcacgt
      421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      841 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      961 tccttcggtc ctccgatcgt tgtcagaagt aagttggcag cagtgttatc actcatggtt
     1021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1141 ccggcgtcaa cacgggataa taccgcacca catagcagaa ctttaaaagt gctcatcatt
     1201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacgaaaa
     1381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttacca gggttattgt
     1441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1501 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1561 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattttataa accgtggagc
     1621 gggcaatact gagctgatga gcaatttccg ttgcaccagt gcccttctga tgaagcgtca
     1681 gcacgacgtt cctgtccacg gtacgcctgc ggccaaattt gattcctttc agctttgctt
     1741 cctgtcggcc ctcattcgtg cgttctagga tcctccggcg ttcagcctgt gccacagccg
     1801 acaggatggt gaccaccatt tgccccatat caccgtcggt actgatcccg tcatcaatga
     1861 accggactgc cacgccctga gcgtcaaatt cctttatcag ttggatcata tcgg
//
LOCUS       DAZGCS010000301         1895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGCS010000301 DAZGCS010000000
VERSION     DAZGCS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.306"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..454
                     /gene="etgA"
                     /locus_tag="ACHK01_005284"
     CDS             <1..454
                     /gene="etgA"
                     /locus_tag="ACHK01_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO8100025.1"
                     /translation="KIILSIILISNSCYASDCFEITGKAYNIDPLILKAIAWNESKNK
                     NGIKSKINKNGTYDIGIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASNIK
                     SRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(451..1488)
                     /gene="escU"
                     /locus_tag="ACHK01_005285"
     CDS             complement(451..1488)
                     /gene="escU"
                     /locus_tag="ACHK01_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO8100026.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGHSY
                     ANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARSLYKNIHKGQYITEDFFEPVA
                     QLIRIAIDLDY"
     gene            complement(1481..>1895)
                     /gene="escT"
                     /locus_tag="ACHK01_005286"
     CDS             complement(1481..>1895)
                     /gene="escT"
                     /locus_tag="ACHK01_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO8100027.1"
                     /translation="ITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQADIAFNR
                     ALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLPVKSLI
                     AIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
BASE COUNT          690 a          329 c          292 g          584 t
ORIGIN      
        1 aaaaattata ttgagtatca ttctcatttc aaattcttgt tatgcttctg attgttttga
       61 aattactgga aaagcatata atatagaccc attaatatta aaagcaattg catggaacga
      121 atcaaaaaac aaaaacggaa taaaaagtaa aataaacaaa aacggaacgt atgatatagg
      181 tataatgcaa ataaattcat ctcatttaga tttattaagc aaatttaata tatctgaaga
      241 tgatttattg aatgatgcct gtataaacat atctgttgct ggttatatct tagcctctaa
      301 cataaaatcc agggggaata catgggatgc agttggtgcg tataatgcag gctactttaa
      361 cacaccaaat gcagtagaac tcagaagaca atatgcaatg aaaatatata aaacatacac
      421 taaattaaaa aacaatgaac aaattatcga ttaataatca agatctattg ctatacgaat
      481 caattgtgct acaggttcaa aaaagtcttc tgttatgtat tgtcctttat gtatgttctt
      541 ataaagactt cttgccaaag gaatatcttc aatcactgga atatcataga gttcagccag
      601 tttaatgatc tgtagagctt tggcatcttt tcctgtttca ataactaaag gcaatggagt
      661 ctccccgagt ttatagtata ggcaaatcgc aatgtgagtc gggtttttga cgatcacggt
      721 tgattttttg atgttattag ccagacttcc actttgtatc tcagaatgaa ggcgacggcg
      781 ctccccttta atttcagggt taccatcagt atccttggct tctcgtttca cctcatcttt
      841 actcattttc attttcttca gtccctcatg cttctggaac cagaaatcaa atactgaaaa
      901 gagtagatat ccgaataaca cgcctttcca taaaagaaaa acaaaaaagg caacaacgac
      961 aagagtttga tatgcgttca gtcttgtgaa atttgcaaac tcatttgcat atgaatgccc
     1021 cataaagtaa aaaatgagag taattatcac taacttaaat actgacttaa gcagctcaaa
     1081 aatactcttt acagaaaaaa tatttttcag gttatttttt acactaatct tctgagccga
     1141 tggttttatt ttttcaaccg caaagatgaa tcctgtttgt gatacaccag ttgtaacagt
     1201 tccaacaaag acaatcaaag aaattggcaa aatatacaat aaaaatatgt ttaataccgc
     1261 acctaatatt tcacgaatgg catacaaaaa cggtctattt agcgagtcta tagtcgtatt
     1321 aactaactca acaacctcaa cgaaaaaact cgcaccatat aaagaaaaaa atgaaaataa
     1381 gattaatgac tgcactgcag ccattacctc ttcacttttt gttacatcgc ccttcttttt
     1441 tagatccctt agttttttgg gtgtgggctt ttctgttttt tcactcatta atcatgctcg
     1501 gtaacgatct gtcaattatg tcagaatgaa tatttgccgt aataaaatca gggaaaacat
     1561 gaataactaa taacaatata aagattgcta tcaaactttt taccggcagt gataatgtga
     1621 atacgtttag ctgaggcgct gttttattga gaaacccaaa ccccatatca cataagaaga
     1681 taccgataat catgggaatt gaaaatgata acatcagttt aataaatgaa tcccatagcg
     1741 aaaataaaaa atctactaaa gcacgattga atgcaatatc ggcttgtaat ggtaatatct
     1801 cgtaggataa atacaactta tccagaacgc tttgtatccc accatgaata acaaagatca
     1861 cagtgataaa ttggtataaa ataacaccag tgata
//
LOCUS       DAZGCS010000302         1883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGCS010000302 DAZGCS010000000
VERSION     DAZGCS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.307"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1883
                     /locus_tag="ACHK01_005287"
     CDS             <1..>1883
                     /locus_tag="ACHK01_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO8100028.1"
                     /translation="AIEAGDECRARAKFHWQFLEHYPAAQDCAYKFIVCEDKPGIPRP
                     ALDSWDAEYMQENRWDEESASFVPVETESYPMNVTFDKLAPEVQNAVMVKFDTCENIT
                     VDMVISAQELLQEDMATFDGHIVEALMKMPEVNTMYPELKLHAIGWVKHKCIPGAKWP
                     EIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQYTENSTGKISPVIAAIHREYK
                     QTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDAL
                     RYDRQTIFGLVRERPIDIHKDPVALNKYICEYLTTKGVFENEETDLGTVDVLQSSETQ
                     TDAVETEVSDIPKNETAPEAEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALSAG
                     ATEITKEEYFARKNGTYSGSQQNTGASDTTAQPGSVKVTADEVNKIMQAANISQPDAD
                     ELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQKAEQNSPNTQQNEP
                     ETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFDGENQPEVQENDPE
                     EMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMIDLETM
                     GKNP"
BASE COUNT          578 a          451 c          478 g          376 t
ORIGIN      
        1 gccattgaag ccggtgatga atgtcgcgcc cgcgcaaaat ttcactggca attcctggaa
       61 cattatccgg ctgctcagga ctgcgcttat aaatttattg tctgcgagga taaacctggc
      121 ataccccgcc ctgccctcga ttcatgggat gctgaatata tgcaggaaaa ccgctgggat
      181 gaggagtctg cttcttttgt cccggttgag actgaatcct atccgatgaa cgtcactttt
      241 gacaagctgg cccctgaagt acagaacgct gtcatggtta agttcgacac atgtgaaaac
      301 atcaccgttg atatggtgat tagcgcacag gaattgttgc aggaagacat ggcaacattc
      361 gacggacata tcgttgaagc gttgatgaaa atgccagaag ttaacaccat gtatccggag
      421 cttaagttgc acgccattgg gtgggttaag cataaatgta ttcctggtgc taaatggccc
      481 gaaattcagg cagagatgcg catctggaaa aaacgtcgcg aaggtgaacg caaggaaacc
      541 ggaaaataca cgtctgttgt tgatctcgcc cgcgccagag ccaatcaaca gtacactgaa
      601 aattcaacag gaaaaatcag cccggtcatt gctgccattc atcgcgaata caagcagaca
      661 tggaaaacac tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga
      721 aacattgacg gcagcatcca tcgctgggca aaaaatgaag ttatcgacaa cggccgcgaa
      781 gactggaagc gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc
      841 cagactattt ttggccttgt ccgtgaacgt ccgatcgaca ttcacaaaga tcccgtagca
      901 ctgaacaaat atatctgcga atacctgacg acaaagggcg tgtttgagaa tgaagaaaca
      961 gacctgggca ctgttgatgt tctccagtca tcagaaacac aaactgatgc agtggaaact
     1021 gaggtatctg atatcccaaa aaatgaaacc gcgccggaag ctgaaccatc tgtagagcgt
     1081 gaggggccgt tctacttcct cttcaccgac aaggatggcg aaaaatacgg tcgcgcaaac
     1141 aaactttctg gtctggataa ggcgctgtct gctggggcta ctgaaatcac gaaagaagaa
     1201 tatttcgccc gcaaaaacgg tacatactca ggttcacaac aaaatactgg tgcatctgac
     1261 acgaccgcac aaccagggtc agtaaaagtt accgctgacg aagtaaacaa aattatgcag
     1321 gcagccaata tcagccagcc tgacgccgat gaactgcttg cagtatcacg tggtgaattt
     1381 gttgaaggga ttagcgaccc gaatgatccg aaatgggtta aggggatcca gacccgcgat
     1441 tctgtgaacc agaaccagca agaaacggaa cagaacgacc agaaagcgga acaaaacagc
     1501 ccaaatacgc aacaaaacga gccagaaacg aaacaacctg aaccagtagt gcaacaggaa
     1561 ccggaaaaga tctgcaccgc ctgcggtcag agcggtggcg gcaactgccc tgattgtggt
     1621 gcggtgatgg gtgacgcaac ataccaggaa atattcgatg gagagaatca gcctgaagtt
     1681 caggaaaatg atccggagga aatggaaggc actgcgcatc agcacaagga gaacactggc
     1741 ggcaatcagc atcatgccag cgatagtgaa actggcgagg cgtcagatcc cttaattaag
     1801 gcgaacggtc atcataatct cacatccacc agcagagcgg ggattcatct gatgatcgac
     1861 cttgaaacca tgggaaaaaa tcc
//
LOCUS       DAZGCS010000303         1873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGCS010000303 DAZGCS010000000
VERSION     DAZGCS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.308"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..412)
                     /locus_tag="ACHK01_005288"
     CDS             complement(95..412)
                     /locus_tag="ACHK01_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100029.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAITKQTMREFDETCLQPAPV
                     MSPERIRALREREHLSQPVFARYLNVSKNLISDWERGVKRPGGAALRLLSVVEKNGIQ
                     VIS"
     gene            complement(405..776)
                     /locus_tag="ACHK01_005289"
     CDS             complement(405..776)
                     /locus_tag="ACHK01_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8100030.1"
                     /translation="MRIFKNAWFERFARKHRISDKSLRKIVEQADKGIISANLGSGVI
                     KQRLARSGGGKSGGYRTIIFYRVAEKAFFIYAYAKNERENITAIEENAFRKAAPHVLN
                     LTDEQLAQLIQQGQFTEVPNE"
     gene            1033..1227
                     /locus_tag="ACHK01_005290"
     CDS             1033..1227
                     /locus_tag="ACHK01_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100031.1"
                     /translation="MKIPASLIPGFYESTRPVVVYRNSDGSFQCGFVMRSNEVVVSLS
                     LLSEVRELAGLPVDDIQKQL"
     gene            1281..1550
                     /locus_tag="ACHK01_005291"
     CDS             1281..1550
                     /locus_tag="ACHK01_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100032.1"
                     /translation="MAHDSRLHNSAALASRQGRCSHVFKTDWFQHPLCTEEQAEWLIQ
                     CYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            1552..>1873
                     /locus_tag="ACHK01_005292"
     CDS             1552..>1873
                     /locus_tag="ACHK01_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100033.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVI"
BASE COUNT          448 a          443 c          453 g          529 t
ORIGIN      
        1 gcgggggaga aatccctcgc cacctctgat gtgtcaggca tcctcaacgc acccgcactt
       61 aacctgcttc ggcgggtttt gttgtattag aatatcagga tattacctgg atcccgtttt
      121 tctcgacaac tgaaagaagc cgaagagctg cacctcccgg gcgtttcact cctcgttccc
      181 agtctgatat caggttttta ctgacgttga ggtatctggc aaaaacaggt tgagacagat
      241 gctctcgttc tcgcagtgca cggatccttt ccggagacat taccggcgca ggctgaagac
      301 aagtttcatc gaattcgcgc atagtttgtt ttgtgattgc accgatatca tggagtgact
      361 ccatcatttc atgtacagat gcaagtgcat cactgcggta atttttactc attgggtacc
      421 tccgtgaact ggccttgttg aatcaattgt gccagctgtt catcagtaag attgaggaca
      481 tggggagcgg cttttcgaaa agcattttcc tctatagcag tgatattctc tcgttcattc
      541 tttgcgtatg cgtagatgaa aaaggctttt tctgcaacgc ggtaaaaaat tattgtccgg
      601 taaccgcctg attttccgcc accacttcgg gctaatcttt gtttaatgac accactaccc
      661 aaatttgcgg atatgatccc cttatcggcc tgctccacga ttttgcgcag cgatttatcg
      721 gaaatccgat gttttcgggc gaaacgttca aaccaggcgt ttttgaaaat tcgcatacct
      781 gcctctgcat tttatataac acatagtgtt acaccatgtg gactatggag gcaatcttta
      841 tttgagaatg cttgctgatt tatcggtgtt caaaaaatag acgtaattca gattgtataa
      901 acggcagaca gaaaaagtca ttgttcttgc gcataaaatc ctcagattta ctgagtgaaa
      961 tgcgtctctt ttcatggtta tagtccgaga gaagcgataa tgcatttcat ttcctttacg
     1021 tcaggaggtg ttgtgaaaat cccagccagt ttgatcccgg ggttttatga gagcacgcgc
     1081 ccggttgttg tttacagaaa cagtgatggt tcttttcaat gtggttttgt tatgcgaagt
     1141 aatgaggttg ttgtaagcct ttctcttctt tctgaggtaa gagagttggc aggattacct
     1201 gttgatgata ttcagaagca gctataaaat caggctgact tgaaccgtcg ctgagaaaca
     1261 ccgtgccacc ggagatcccg atggcgcacg atagcagatt gcacaattct gccgcccttg
     1321 ccagtaggca agggcggtgt tcgcacgtat ttaaaaccga ctggttccag catcccctat
     1381 gcactgaaga acaggccgaa tggctgatcc agtgttaccg caggcgtggt tacgagtttc
     1441 agaaagatct cagcttcgat cgtcgtcact ggataatctc cgccaggctc ccttattccg
     1501 aacgcccacc gcgtccgtcc cgcacattcc agcagcgcat ctggaggtaa cgtgcgggta
     1561 ttacttcgac ctgttctggt accggaactc ggtctggtta tcgttaagcc aggccgtgaa
     1621 tcaatgtcag tattccataa cggcagaata ttggtggagc cggaaccgaa aaacatgcgc
     1681 ggtctgccgt ccggagtcgt tcctgccgtt cgccagccgc tggcagagga taaaacatta
     1741 ctgccatttt tcagcgatga gcgggttatt cgtgcagcag gtggtgcagg tgcactgtct
     1801 gactggttat tacgtcacgt gaaatcctgc cagtggccac acggcgatta tcatcacagc
     1861 gaaacagtca ttc
//
LOCUS       DAZGCS010000304         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGCS010000304 DAZGCS010000000
VERSION     DAZGCS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.309"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1192
                     /locus_tag="ACHK01_005293"
     CDS             <1..1192
                     /locus_tag="ACHK01_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO8100034.1"
                     /translation="QYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSN
                     GRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALK
                     DPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPARVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYCHADGTEMTISRVCW
                     DTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWD
                     NKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVN
                     GYSR"
     gene            1176..1382
                     /locus_tag="ACHK01_005294"
     CDS             1176..1382
                     /locus_tag="ACHK01_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO8100035.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTVASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            1379..>1863
                     /locus_tag="ACHK01_005295"
     CDS             1379..>1863
                     /locus_tag="ACHK01_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8100036.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     L"
BASE COUNT          434 a          431 c          599 g          399 t
ORIGIN      
        1 gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg agaaggacag
       61 ccccgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgaact
      121 ggaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc gtgacggtct
      181 gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca cgttccatat
      241 ctggacggcg tacagtccgt ttaccacctg ggtacagata gtctatgact ggctggatgc
      301 actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg agacctggga
      361 agaagccgtg ggcgaaaaac tcgatcacca ggtactgatg gataaggtcg tgcattacac
      421 ggcggcggta cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg
      481 ttttgagatg tatgtctggg gatgggcacc gggagaggaa gcttttctgg tggataaaat
      541 catcattatg ggccgtcccg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa
      601 caaaaaatac tgccatgcag acggaaccga aatgaccatt tcccgtgtct gctgggacac
      661 cggggggatc gatggtgaaa ttgtctatca gaggtcaaaa aaacacggtg ttttccgggt
      721 gctgccggta aaaggcgcat ctgtctatgg caagccggtg atcaccatgc cgaaaacccg
      781 caatcagcgg ggcgtgtatc tgtgtgaagt ggggacggac accgcaaaag aaattctcta
      841 tgcccgtatg aaagccgatc ccactcctgc ggatgaagcc acgtcgtatg ccatccgttt
      901 tcctgatgat ccggagattt tttcgcagac agaggcgcag caactggtcg cggaagagct
      961 tgtggagaag tgggaaaaag gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa
     1021 cgaagcgctg gactgcctgg tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg
     1081 gcagcttgat ctggctgtac tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct
     1141 gaaagaactg gcagcgaagc tgtccggagg agtgaatggt tacagtcgct gaactacagg
     1201 cgctacgtca ggcgcgcctt gatttattaa ccggtaaacg ggtggtgtct gtccagaaag
     1261 atggtcgcag aattgaatat acggtggctt ctctggatga gcttaaccgg gcgatcaatg
     1321 atgcggagtc ggtactgggg acaacccgac gtcgccgtcg tccgctggga gtgaggttat
     1381 gaaacgaacg cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta
     1441 caacgggggc ggcgcaggat ttggcgggca aatggcggag tggttgccac cggcgcagag
     1501 tgccgatgca gccctgctgc ctgcgttgcg tctggggaat gcccgggcag atgatctggt
     1561 gcgcaataac gggatagcgg ccaatgcggt ggccctgcat aaggatcata ttgtcgggca
     1621 tatgtttctg atcagctacc gtccgaactg gcgctggctg gggatgcggg agactgcggc
     1681 aaaaagtttt gtcgatgagg tggaggcggc ctggtcggaa tacgccgaag ggatgtctgg
     1741 cgagatcgac gtggaagaga aacgcacgtt tacggaattt attcgtgaag gtgtgggcgt
     1801 tcatgcgttt aacggcgaaa tctttgtgca gccggtctgg gatacggaga gcacgcaact
     1861 gtt
//
LOCUS       DAZGCS010000305         1858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGCS010000305 DAZGCS010000000
VERSION     DAZGCS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.310"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..271)
                     /locus_tag="ACHK01_005296"
                     /pseudo
     CDS             complement(59..271)
                     /locus_tag="ACHK01_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            421..1089
                     /locus_tag="ACHK01_005297"
     CDS             421..1089
                     /locus_tag="ACHK01_005297"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8100037.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1455..1568
                     /locus_tag="ACHK01_005298"
     CDS             1455..1568
                     /locus_tag="ACHK01_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO8100038.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1637..>1858
                     /gene="ydfK"
                     /locus_tag="ACHK01_005299"
     CDS             1637..>1858
                     /gene="ydfK"
                     /locus_tag="ACHK01_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO8100039.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLN"
BASE COUNT          604 a          396 c          321 g          537 t
ORIGIN      
        1 tattacttta atcaaaagtt gacgtgccag attcgaagta aatttataaa atatcaaatc
       61 aatatacatt ttgcaccttc gagatagtaa tgactgttac tgtaacatga gttacaaaca
      121 gcttcatacc cagagctact gatgtatcaa ctcgattcaa caatacccga tatttattcc
      181 atgcctccag caacgatctt tcttcctccg ttgcgatttc cagatctaca gcatcctgca
      241 gtggcgcaat atactcactg aattcctgga tgtagaactg tgtggtgacg gtcttccagc
      301 cattcagctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca
      361 gcatttaacc ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataac
      421 atggtgaaat taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc
      481 ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct
      541 acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt
      601 ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg
      661 gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa
      721 cataaaatta accatgatgt ttttgaacct tatcccgcgg cgttacatgg tcaatttgat
      781 tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt
      841 gtaatacgca atgcggcgca ggccttaact gacgatggaa ctctatacgg agccacaatt
      901 cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag
      961 aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag
     1021 catttcgaga atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg
     1081 aaaaaatagc atccaaccgc agcacgttct tgcataaggc gtgtctgcgg tttttcaact
     1141 attcagatac atcactccca tcacattcat tcctccgcat cataggcata taggctatat
     1201 caccttgata tttttcttct tcagataaaa actgttatct atgtatactt ttaaacccaa
     1261 tccgtgtaga gtctctgcat aagatagttt gcagttgcca cttcagcttg cgccataaac
     1321 cgcctgattt ttgccgccac ctgttagcat tcctgtatac ctgaaacgac aatgtttatc
     1381 tacgaacttt aagaacaccc aggataaaaa ttgtcaacta tatcatatat aacacattac
     1441 taattcgagg ctatatgaac agcatactga taatcacttc gctccttatc atattcagca
     1501 tttttagtca tgctctaata aaattaggga ttggcatatc caataaccca gacaaaaccg
     1561 atgtataagt caacatatcc tgaatcagac atacaatatc gcaatgaaaa tcaataatat
     1621 tttaaggaat atcttcatga aatcaaaaga caccctaaag tggttccctg cgcagcttcc
     1681 tgaagtaaga attatcctag gggatgctgt agtggaagta gcaaaacagg gaagacctat
     1741 caataccaga acattgcttg attacattga aggaaacata aagaaaaagt catggctgga
     1801 taacaaagaa ttattacaaa cagcgatatc agttcttaaa gacaacaaaa atttaaat
//
LOCUS       DAZGCS010000306         1856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGCS010000306 DAZGCS010000000
VERSION     DAZGCS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.311"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..282
                     /locus_tag="ACHK01_005300"
     CDS             <1..282
                     /locus_tag="ACHK01_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="HFO8100040.1"
                     /translation="FGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVIS
                     KRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            299..559
                     /locus_tag="ACHK01_005301"
                     /pseudo
     CDS             299..559
                     /locus_tag="ACHK01_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(701..1825)
                     /locus_tag="ACHK01_005302"
     CDS             complement(701..1825)
                     /locus_tag="ACHK01_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO8100041.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          539 a          426 c          373 g          518 t
ORIGIN      
        1 ttcggtgaac gcactctggc cacactggag cgtcttctga gcctgctgtc ggcctttgag
       61 gtcgtggtat ggatgacgga tggctggccg ctgtatgaat cacgcctgaa gggaaagctg
      121 cacgttatca gcaagcgtta cactcagcgc attgagcgac ataatctgaa tctgagacaa
      181 catctggcaa ggctgggacg gaagtcactg tcgttctcaa aatcggtgga gctgcatgac
      241 aaggtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt cattacctga
      301 gaataatacc tcctgggtaa gtgagcagat actttcagcg cctccgaaag atgtaaatat
      361 tcgacttgga gtcggttctt tagaaggtac aaccgtctca catgttcagc gattgcatca
      421 ttcgttaatc gcagctggtt tggaaagtaa cctcactgtc tataccggtg gtcacgatta
      481 tgcctggtgg cgcggagcaa ttattgatga attagcaaat tataattgca ggaaggtatc
      541 aaataataac tctgtgtaaa atatcgtttt atcgtcagat aactctttct gcaatattat
      601 tatgggagac tatctcattt atttagtgat gtccttcatt tatgtgatga aggacattat
      661 attactcata acatttctcg ttgaaaattt cagcaatacg ctacaattgt ttcccgttgt
      721 tttttaattt tctcatcaaa acctctgcaa tctctgcagg actgggttgt tcagccattt
      781 cagcggctac ctgatctgaa cagaagcgta gtgaatcatc ataaagaagg cgatttacca
      841 aatcactggt cagtccatgc ttgtccggaa taatcccaca cccacgcttc gcaacaattt
      901 ctgcattaac agagcgatct gcaccttcgc caaacacaat ctgtggtatt ccactataca
      961 gcgctgtcag ggtatttcct gcgccaccgt gatgaataaa tccatcagct ccattaagga
     1021 atacacccat aggtatccag tcaaccagac gtacatttga tggtagtttt cgtaaaccag
     1081 tacgagcatt tattgcaagt tgcaaaatga tatcagcatc aacttcattt gcagaatcca
     1141 taacccatga aatcagctcc agaccatcaa ccattggttt tacagtgcca aggctgataa
     1201 gtaaacgttt ccgatcagaa tccctgtccc accatgtttc ctttacagca cctccgttat
     1261 aaggaatata tctcattgag ataaccggtt ctccagcatt tttaagaatg ctcatacttg
     1321 ggggagcaac atcaatccat gctaaatcac atagtggacc gctgactcca tgccgcctgt
     1381 aagcattgct taaagaacgg tttaacatct gaatatgggc agatgtatgc gcgaatccaa
     1441 cagccagcat cactgaagga attctatatt tagcagcaac caatgggcct gccggatcaa
     1501 gagggggata gactattaaa tctggcctcc attttcctgc aaaatcgatg aggttatctg
     1561 ccatttcatc gctaaaaaaa gagaaatgac caataatatt actttttttc ctcaactctt
     1621 cctgatggcg atatccagcc tctgaatcta aacctggaac tgcatcaaaa acaaccagtc
     1681 cagcttctgc tgctttattc gcgaatttgc cagcgctact aattactaca tcatgtccaa
     1741 ttacacgaaa ggcctgagcc agagaaatca atgggtataa caaaccgtac agcggtgggc
     1801 caataaaaag aatacgtttt cgcattaaaa aagtccgtct tctgcttacc tgttta
//
LOCUS       DAZGCS010000307         1830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGCS010000307 DAZGCS010000000
VERSION     DAZGCS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.312"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..308)
                     /locus_tag="ACHK01_005303"
     CDS             complement(<1..308)
                     /locus_tag="ACHK01_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8100042.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     "
     gene            complement(836..1021)
                     /locus_tag="ACHK01_005304"
     CDS             complement(836..1021)
                     /locus_tag="ACHK01_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8100043.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(1243..1356)
                     /locus_tag="ACHK01_005305"
     CDS             complement(1243..1356)
                     /locus_tag="ACHK01_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100044.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            complement(1577..>1830)
                     /locus_tag="ACHK01_005306"
     CDS             complement(1577..>1830)
                     /locus_tag="ACHK01_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO8100045.1"
                     /translation="VPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEA
                     IRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWDIDR"
BASE COUNT          473 a          513 c          344 g          500 t
ORIGIN      
        1 ttttttgacc gcgcgtttcc ggacgtattc tgttctcctg tccctttata tcgtcggaat
       61 acccgccgct cttcaaatcc cattcccaac tcagaatgta gtctgttgac cgcttgtttt
      121 atttcggtca ggttcaccgg tgaaaccgga gtccggcgcg ccttacgcaa acactctgct
      181 cgtttctgtg ccgccacttt tcttttctgg tcatcactta gctgtaccat cacttttgcc
      241 catcgttcag ctgctctccg gtacagtcct tttttctcca gacattctgc cacgtgatca
      301 tgtagcataa gtgacctccg attatctaca gactgccatc ctgaatttac cttcccttaa
      361 tgaaataaca ataaaaaaca aaccacgcaa aaacaataaa acaacacaca aaaaaaacta
      421 aataataaac aaaaataatc accttatttt attatttttt gagggagcaa ttactgaaca
      481 aaaaacgctg actatatact caaaaccaaa caactattct gccaatcagg tatcatggca
      541 acacacggaa ttaccgtgtt tttgccttct ctgcccatac aatacgggca tatacttcat
      601 tctctattgt aatatttcta tccatgtgcc ccactccatt tacctgtaaa taatattcaa
      661 aatatttatc acagaaatcg tttttggcca tgaactgagc acactataaa gtccggaact
      721 gactctttgt taaattacct taacgttacc agtaacacct tcataacaaa acatcacggt
      781 atacactggg tacggatata ttcctgtgct ccttccagtt gcttctgcat tgccatcagc
      841 cgttctctga ggatgaaata atcccgttca gcggtgtctg ccagtcgggg gccggttgca
      901 ttatccacgc cggaggtgcc ggtggcttca cgcacggtac cggagcaggt ggcgttgatc
      961 cgcaggcgct tacgaccagc ggcaacatca gcacgcagag tttcattttc agctctcgca
     1021 tcggctaatt ccctcgagta tctggcatca agtgcagcga catcacgctg gcgtatctgc
     1081 atatcagtaa ttgtcgcgtt cgccagctcc agctcactgg cttttttatc gcgctgctct
     1141 ttgtagatga tggcgtgatc acggtaatga ttcagcccca gactaagcgc accacaggcc
     1201 accagcagga caatgataac cacgcacaga acacggttca tatcaccacc aacggattgc
     1261 ccagaccaga acagcaatgg ccacaatacg aatggcaaaa gctgccgctc ttgttaaatc
     1321 cagactggct ggcgtctcca cttcaatgcc tttcataatg gacaacctca gaaagaatct
     1381 tttatacttc ctcacaggga aagtacctcc ctacccataa tttctccctt gccttactca
     1441 aggtcagaaa acacaaaacc ccgcttgctg ccaacaaacg gggtttttac ttttattcac
     1501 ttaggtttta ccagttttca ggatttcgtg ttatcccccc gcgttggcca acgtcatttt
     1561 tcaggaaaat attctgctat ctgtcgatgt cccagcacgc cagcgcgctc tcctggtcac
     1621 gccgtgagac ctgaccgtag caattatttg aacggatacg gcagtctcta ccaccgtcct
     1681 taatccacca gcgaatcgcc tcacaggcac cttttcgatc gcctgcatta attcgtttat
     1741 aaaacgtcga cgggaagcac ttaccggggc caatgttgta cggacagaat gacgcgatcc
     1801 ccgctttctg gggttcggtc agcggcaccc
//
LOCUS       DAZGCS010000308         1819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGCS010000308 DAZGCS010000000
VERSION     DAZGCS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.313"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1194..1796
                     /gene="fimB"
                     /locus_tag="ACHK01_005307"
     CDS             1194..1796
                     /gene="fimB"
                     /locus_tag="ACHK01_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO8100046.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          547 a          327 c          387 g          558 t
ORIGIN      
        1 tcaatccatt ttacccagag tcataaaata gaatgtctgt tgatctcatt ttcatattcc
       61 tttcagactg aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat
      121 agattgcgag atgccctgtc tcattgacta tcttggtgca cctttatacc tgttatacca
      181 gatcaaaaat cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat
      241 gtcgatattt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggaggcct
      301 tcacaaacca aagattgttg tttaattatc tgttttataa gtaatttatt taaagtataa
      361 acaaggaaag atttgcaagt taactcagtg agcgtggtga aaatcagtgt ttacccgcca
      421 tcaggctgag cataattctc atcatgaaat atgtttcctg gtttgtggct tgtaactggt
      481 cacttctgaa gtcgatctgg agaggcttgt tgatgttggt gttttcagga tgatgtttca
      541 cttagtttgt ttgccgtatc gcccggcgaa tggctgtgat tgaggaaggt ttaagtcgta
      601 gtgaccaaag ctatatttac caacgaatgt agatgaaaaa atcatctcct gcgttcccca
      661 tatctccagg ataaaaagga atgtaacaat ctcatggcgt aagctgacga atcagccagg
      721 aataatcgct agggacctaa gaattagcat gataatagcc actaagaaat tactgcgctc
      781 catgaaatag ccattttgtg gcaaatggag ttgactaata atgtcatatg tgagacggct
      841 agttgaacga atattaaatt ttgctgaatt ttttatgttg attttacttg ttacagaaca
      901 tatcacatga tatatagata agattagttg cattaatgat gagggttatt attagattcg
      961 tatccgattg ataaatatat aaaggtacat agcatgcaag agcatggcgt ttgtatggca
     1021 acgttattat aattaactgt tgctactcca tttaagttca ctcagaagaa ctggtccact
     1081 tacgttagtt attaagcaaa cgttcgcttt tataaacata atcaggataa aaatgttgga
     1141 ttattgctaa cccagcacag ctagtgcgcg tctgtaatta taagggaaaa acgatgaaga
     1201 ataaggctga taacaaaaaa aggaacttcc tgacccatag tgaaatcgaa tcactcctta
     1261 aagcagcaaa taccgggcct catgcagcac gtaattattg tctgactttg ctttgtttta
     1321 ttcatggttt ccgggcgagt gaaatttgtc gattgaggat ttcggatatt gatcttaagg
     1381 caaagtgtat atatatccat cgattaaaaa aaggcttttc aacaacgcat ccgctattga
     1441 acaaagaagt tcaggcttta aaaaactggt tgagtatccg tacttcgtac ccgcatgctg
     1501 agagcgagtg ggtattttta tctcgtaagg gaaatccgct ttctcggcaa cagttttacc
     1561 atattatctc gacttccggt ggtaatgccg ggttgtcact ggagattcat ccgcacatgt
     1621 tacgccattc gtgtggtttt gctttggcga atatgggaat agatacgcga cttatccagg
     1681 attatcttgg gcatcgcaat attcgtcata ctgtctggta taccgccagc aatgcagggc
     1741 gtttttacgg catctgggat agagccagag gacgacagcg tcacgctgtt ttatagagat
     1801 agttgagata ccagggatg
//
LOCUS       DAZGCS010000309         1777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGCS010000309 DAZGCS010000000
VERSION     DAZGCS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.314"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..831
                     /locus_tag="ACHK01_005308"
     CDS             <1..831
                     /locus_tag="ACHK01_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8100047.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
     gene            883..1266
                     /gene="gpFI"
                     /locus_tag="ACHK01_005309"
     CDS             883..1266
                     /gene="gpFI"
                     /locus_tag="ACHK01_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8100048.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRQCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            1259..1612
                     /locus_tag="ACHK01_005310"
     CDS             1259..1612
                     /locus_tag="ACHK01_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8100049.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1627..>1777
                     /locus_tag="ACHK01_005311"
     CDS             1627..>1777
                     /locus_tag="ACHK01_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100050.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRV"
BASE COUNT          394 a          407 c          576 g          400 t
ORIGIN      
        1 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac
       61 cagcaggcgg ttgagcgtct tcccggtgaa gatccggctc aactgaacga cccggcctac
      121 cgtcgtctgc gtatcatcac cgataacctc aaacaggaag agcatgccat tgtccaggtg
      181 gaagaaatgc aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggatcag
      241 tttgatactg tcgaggtgga ttttggacgc tctgaaggaa ataacattga gcaggccgac
      301 ggtaaaaaat ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc
      361 tactgcgatc aggccagcgg tcttgtgaat attgccatta tggacggtac tgtctggcgt
      421 ctgctgaatg gttttaagct gttccgcgaa aaactggata cccgtcgcgg ctcaaattca
      481 caactcgaaa cggcagtgaa agacctgggg gcggtggtgt ctttcaaagg gtattacggc
      541 gatctggcca ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt
      601 tatctgccgg agggcacgct ggtcctggga aatacggctg cagatgggat ccgttgttac
      661 ggtgccattc aggatgcgca ggcgttgtcc gaaggtgtgg tggcttcttc ccgttacccg
      721 aaacactggc tgaccgtggg cgatccggcc cgtgaattta ccatgacgca gtccgcaccg
      781 ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc aggtgaaata atccgtgagc
      841 gggggcgaaa tgcccccgtg tcttttttca cagggggctg atatggcaac aaaagaagaa
      901 aatcagaaac gtcttcgtca actggctggc ctgctggggc gcgaggcgga tatgtcgggg
      961 agtgctgcgg atattgcgca acgtgtgtct gagtgggaag aggagcttgc tgcttcccgg
     1021 gagggcatta tgcctggtga tgagagcggg cctgagcaaa atcacacaga cgatggtgag
     1081 cagttgcaca acactgatgc tacggatgat gttaaagcgg tccgtgtgcg gcaatgcctg
     1141 catgtgatgg ggtattgccc ggagacaggc cgtcccgttg aactgacgta ccggggcatg
     1201 cgtgttctgg tgccatcacc actggcgaca gccatgatac agcacggaac ggctgagcat
     1261 gcgtgatttt cagaatgcct ttgatgcagc ccttgccggg gtggacagca cgattgttga
     1321 agtgatgggg ctctgtgcgc agttcacctc gggggcacag tgtggcagcg aagttcaggg
     1381 ggtttttgac gatccggagt cgctggggtt tgccggtagc ggggtccgta ttgaaggaag
     1441 ctgcccgtca ttatttgtac ggacggatac ggttcgtgct gtgcggcgtg gtgacacgct
     1501 gaccattaat ggtgagacat tctgggtgga tcgtgtttct ccggatgacg ggggcagttg
     1561 ttatctctgg ctcaaccgtg ggcaaccacc ggcagttaac cggcgacgat aaacgcaggg
     1621 tgaattatgg cgataaaagg gcttgatcag gcgattgaaa atctgagccg ggttcgtaaa
     1681 aacgccattc cggcggcttc agcaatggcc attaaccgcg tggccacaac ggcgattaat
     1741 cagtcttcgt cacaggttgc ccgggagacc agggtga
//
LOCUS       DAZGCS010000310         1768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGCS010000310 DAZGCS010000000
VERSION     DAZGCS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.315"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..151)
                     /locus_tag="ACHK01_005312"
     CDS             complement(<1..151)
                     /locus_tag="ACHK01_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100051.1"
                     /translation="MAIKGLDQTIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRV"
     gene            complement(166..519)
                     /locus_tag="ACHK01_005313"
     CDS             complement(166..519)
                     /locus_tag="ACHK01_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8100052.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(512..886)
                     /gene="gpFI"
                     /locus_tag="ACHK01_005314"
     CDS             complement(512..886)
                     /gene="gpFI"
                     /locus_tag="ACHK01_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8100053.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(938..>1768)
                     /locus_tag="ACHK01_005315"
     CDS             complement(938..>1768)
                     /locus_tag="ACHK01_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8100054.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGK
                     EWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT          414 a          563 c          390 g          401 t
ORIGIN      
        1 tcaccctggt ttcccgggca acctgtgacg aagactgatt aatcgccgtt gtggccacgc
       61 ggttaatggc cattgctgaa gccgccggaa tggcgttttt acgaacccgg ctcagatttt
      121 caatcgtctg atcaagccct tttatcgcca taattcaccc tgcgtttatc gtcgccggtt
      181 aacggcgggt ggttgaccac ggttgagcca gagataacag ctgcccccgt catccggaga
      241 aacacgatcc acccagaata tctcaccatt aatggtcagc gtgtcaccac gccgcacggc
      301 acgaaccgta tccgtccgca caaataatga cgggctgctt ccttcaatac ggaccccgcc
      361 accggcaaac cccagcgact ccggatcgtc aaaaaccccc tgaacttcgc tgccacactg
      421 tgcccccgag gtgaactgcg cacagagccc catcacttca acaatcgtgc tgtccacccc
      481 ggcaagggca gcatcaaagg cattctgaaa atcacgcata aacagccatt ccgccatcaa
      541 cgtgtgtttt tgcatctgag gacataatca gaatcacccg accaacatcc gcaatctcaa
      601 cggattcccc tgtttcacca tcaatgccac agagatggag gcaggtcaga actctgatgc
      661 gcgttaacgc accggatgta tcctcacgaa catcatgagc cgcggtttcc cgctcccgga
      721 tcaccgtatc cccaacctga acatcctcgc cggatgactg tatttcctct tcccattccg
      781 ccacccgctg cgctatctct gcggcactcc cggatatatc cggctcacgc cccagaatca
      841 gggccagttc atcaagccgt ttcagatttt gctctttcgt tgccatatca gccccctgtg
      901 aaaaaagaca cgggggcatt tcgcccccgc tcacggatta tttcacctgt accaccacaa
      961 actcatccgg gtccggcaac accatcagcg gcgcggactg cgtcatggta aattcacggg
     1021 cgggatcccc caccgtcagc cagtgtttcg gataacggga agaggccacc acaccttcgg
     1081 acaacgcctg cgcatcctga atggcaccgt aacaacggat cccatctgca gcagtattcc
     1141 ccagaaccag catgccctcc ggcagataac gtttttcgat accgtcttct gctatataag
     1201 acgttttcgc caccacaatg gccagatcgc cgtaataccc cttgaaggac accactgcgc
     1261 ccagatcttt cactgccgtt tcgagttgag aatttgaacc gcgacgggta tccagttttt
     1321 cgcggaacag cttaaaacca ttcagaagac gccagacggt accgtccata atggcaatat
     1381 tcacaagacc gctggcctgg tcgcagtaga ggtcaagatc atgtgtagga tcgaacgtgt
     1441 cacgatcctg ttttgaccac tccttaccac taccctgagt gatgttattc ttcgtcgacc
     1501 tgccaaaatc gacctcaatt ttctcgaact ggtctccttc catcgtatat ttgccataca
     1561 acacagcatt taccgcctgt atttcttcca cctggacaat cgcgtgctct tcctgtttga
     1621 ggttatcggt aatgatacgc agacggcggt aagccggatc gttcagttga gccggatctt
     1681 caccaggaag gcgctcaacc gcctgctggt aattaaattc gtgtttcggc ttgacgtagc
     1741 ccggacgtaa cacgcgggtt tcaccacc
//
LOCUS       DAZGCS010000311         1743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGCS010000311 DAZGCS010000000
VERSION     DAZGCS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.316"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..1209
                     /locus_tag="ACHK01_005316"
     CDS             16..1209
                     /locus_tag="ACHK01_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8100055.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1595..1743
                     /locus_tag="ACHK01_005317"
                     /pseudo
     CDS             1595..1743
                     /locus_tag="ACHK01_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1660..>1743
                     /locus_tag="ACHK01_005317"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          371 a          447 c          507 g          418 t
ORIGIN      
        1 agttgaaaca ttaaaatgtc acctgggatc gaagatacgc cccaaaaacc actgtcctgc
       61 tggccacttg cgttcagtgc cgggcttctc ggtatcgggc agaacggtct gctggttgtg
      121 ctccctgttc tggtcataca gacaaatctg agtctgtctg tatgggctgc cctgctgatg
      181 cttggctcaa tgctgtttct gccatcgtcc ccgtggtggg gaaagcaaat ttcccgtact
      241 ggcagtaaac ctgtggtgtt gtgggctctg ggaggatatg gcataagctt taccctgcta
      301 gggctgggaa gcgtgctgat ggctaccagc gccataacaa cagcggtggg actgggaata
      361 ttaatcatcg cccggatcgc ctacgggctg accgtgtcgg caatggtgcc agcctgtcag
      421 gtctgggcat tgcagagagc aggagaaggg aatcgcatgg ccgctctggc aaccatcagc
      481 tccggcctga gttgcggcag gctattcggg ccgctgtgcg cggcagcaat gctggccatt
      541 catcctctgg cgccactggg gctgctgatg gcagccccag tgctggcgct gctgatgctg
      601 ctgcggttgc ccggcacacc accacagccc acaccggagc gcaagagcgt cagtctgaag
      661 cgggattgtc tgccttatct gctttgcgca atattactgg ctgcggcggt gagcatgatg
      721 cagcttggac tttcgcctgc ccttactcgc cagttcgcca cggataccac cgccattagc
      781 caacaggtgg cctggctgtt gggtctgtcc gcagtagctg cgcttatcgc gcagttcggg
      841 gtactccgtc cgcagcgcct gactccggtg gccctgctcc tgagtgccgg agtgttgatg
      901 agtggtggtc tggctatcat gctctccgaa cagctatggt tgttttaccc gggctgtgca
      961 gtgctgtcat ttggagcagc tctggcaaca cccgcttatc aacttctact gaatgataag
     1021 ctggctgatg gcgcaggcgc gggctggctc gctaccagtc acacacttgg ctatggactg
     1081 tgcgctttgc tggtgccgct ggtatcgaaa acaggtgtcg caatcgcgct gattatggcg
     1141 gcattatttg caactatatt atttaccatt gtgtctgtat ttatctggca ttaccgtgct
     1201 atcaaataaa gatatgatca tcaccttacc ggagtaactc agaggatatg agagagaacc
     1261 tgcgtctgat gaaagcatgt caggcagtaa cactggcaaa aatgcaccgc atggtacagg
     1321 gtatgttcgg tataaaaccg gccggtgtca cttaagttgc attgaagtca tgctggttgt
     1381 gagtgcaaca tcacatggct ctgtccggat gcctgcagac aatgagaaca gtgcgattac
     1441 tcattgggaa tgagatgaac gcatccccaa cattagatct ggggcaccgt gataaataaa
     1501 ggggaaaatc agagaggtgt cgagcctcgc agatttccgg catcatagtg cccaatacgg
     1561 gatgcataaa actctctcag agtggcgttg acacctgaaa gagctggagc gtgaaaatcg
     1621 tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc gcttattttg cgaaggcgga
     1681 gttcgaccgc ctctggaaaa aatgatgcca ctgctggata agctgcgtga gcagtacggg
     1741 gtc
//
LOCUS       DAZGCS010000312         1726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGCS010000312 DAZGCS010000000
VERSION     DAZGCS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.318"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..493)
                     /locus_tag="ACHK01_005318"
                     /pseudo
     CDS             complement(1..493)
                     /locus_tag="ACHK01_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(596..1722)
                     /locus_tag="ACHK01_005319"
                     /pseudo
     CDS             complement(596..1722)
                     /locus_tag="ACHK01_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          489 a          390 c          263 g          584 t
ORIGIN      
        1 taatatccag catgttaaga agttctggga tagctgtaat ctcattagat ttctcatccg
       61 tcctgatctg tccgatgacc agactgtgca ttgttgagaa cgcactaatg acatgaatcg
      121 ctcccctgcg gcgactcttg tcataagagt gccggagcgt ttttccatca attgcgatga
      181 cgtctttatc atctgaagaa tggcagtcac gcatccagtt aataaagcac tcatgaaatt
      241 ttgcaggact gatacaggat acaactctgg caatagtatc gtgaacagga ataccatttt
      301 caaaatcacc atattgcttc aagaaatcga gatgtgtttc cccgaaatcc tctatatctt
      361 cccaactttc tgcaccagaa ataacggcaa aaatagtcaa tagtaggatg cctgacaatt
      421 tatgctctac tttccaggct tatctgtaat cgggaataat agaaatatgt tccatcaatt
      481 ttttaagttc catttgttca ccttatttat gtaaggagta tttgaacatg tataagcaat
      541 aaaaaacagc ttcaggtaat gaggaatatc tcaattttta aacataaaat gcgaattatt
      601 tagtacaaaa agtgaacttc tgatgatctt tccctgtgtt taacgcagcc ttctttcttt
      661 tcctcttcac ccttaatgtc tttgcaatct cttaataaat tcagtgccat cttctttatt
      721 ccagatatta tttaggctgc gtttcctctt cttatccggc tggcatcttc attcattttt
      781 acatctaaca cccaatgaag actgtgctcg atcagccagt gcgctctgat agcatgtgca
      841 aattctttag catccatatc ctttgatgaa atataatatc ggatgcttac accttctgct
      901 gatttatctt ctttcttctg cctgaatgac aatgctacac aaagcttttt taatcccttc
      961 cattcgaatt caaaatcaca aaagttcagg cgttacgtta ctgacaatat gcaaacgtgt
     1021 ttcttttctt ccatgactta tctcctgcgt actaaacgaa tcgcctttat aattagaaaa
     1081 cacatttaca ggaaattttt cctcgaatgc atgatgtaat ttcccctgat tgccttttac
     1141 tgccagaaga taatctgctt ttttatcttt gatcttcgaa gcgatatctt tctgacagcc
     1201 catagcatca atggttatca aatttttctt taaatacagt aggttaagca actctggaat
     1261 ggctgtaatc tcattacttt tggcttccgt tttcacctgc cccagtacaa caccattttc
     1321 gttcgagaat gcactcacca tatggattgc tccttttctt tttcccttat caaaggagcc
     1381 tcttatggtc tttccatcta ttgctataat ttcaccatca gtgatttcat ggcactcctg
     1441 catccattca ataaacatct tttcaaaggc caaactgtca atgttactca caacgcgtgc
     1501 aatggtgtca tcgaccggaa tgccattatc aaaatcacca tatttcttta gccattcaag
     1561 tctttcatgt ccaaaatctt caatttcctg ccactcatcg gcacctgcaa ttactgcaca
     1621 tacggtgaga aacaaaatag cagataattt atgtttaacc tttccttgtt gtcgtatatc
     1681 agggatcact gaaatataat caagcaaact ttgaatactc acctgg
//
LOCUS       DAZGCS010000313         1684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGCS010000313 DAZGCS010000000
VERSION     DAZGCS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.320"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1223
                     /locus_tag="ACHK01_005320"
     CDS             <1..1223
                     /locus_tag="ACHK01_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8100056.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPE
                     VSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGSKMRTPHLVPLSQQA
                     VAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREQC
                     ISPFEYAKINNPLK"
     gene            complement(1409..1684)
                     /locus_tag="ACHK01_005321"
                     /pseudo
     CDS             complement(1409..1684)
                     /locus_tag="ACHK01_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          458 a          403 c          392 g          431 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggta catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa agagaagact ctggcacttg
      121 gtgtgtatcc agaagtatct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcgga gggggttgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc tttcgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaagta ctcaaaagcc tcgagacaca cgtattcccc tttatcggca
      361 atcgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgccgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat atgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttttcatacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag ccaacaggct gtagccatac
      841 tggcagaact tcagacatgg gcaggtgaaa atggtctgat ctttacggga gcacatgacc
      901 cacgtaaacc aatcagtgaa aacaccgtaa ataaagccct gagggttatg gggtatgaca
      961 caacccagga tgtctgtggt catggattcc gggcgatggc atgtagtgca ttaatagaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gccagatgag ccatcaggag cgtaatggtg
     1081 tacgtgctgc gtatatccat aaagccgaac atctggaaga acgtcgactt atgctacagt
     1141 ggtgggcaga ttttctggat gcgaacagag aacaatgtat cagcccgttt gaatatgcaa
     1201 agattaacaa tccattaaaa tagtaatcat cccgggcagc tagcccggga attattttct
     1261 gaatattttt ctttgtgagt aactggaaaa aggatagcga agtaaattac tttaatgaat
     1321 tattccaggg gctcattaaa gttctttcgt aatactttgt agtcaaacac gttatggctg
     1381 gcatcaaaaa ccaggtaatg actccaactt attgatagtg ttttatgttc agataatgcc
     1441 cgatgacctt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc cgtcccagcc
     1501 ttgccagatg ttgtctcaga ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa
     1561 cgtgcagctt tcccttcagg cgtgattcat acagcggcca gccatccgtc atctatacca
     1621 cgacctcaaa ggccgacagc aggctcagaa tacgctccag tgtggccaga gtgcgttcac
     1681 cgaa
//
LOCUS       DAZGCS010000314         1675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGCS010000314 DAZGCS010000000
VERSION     DAZGCS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.321"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..648
                     /locus_tag="ACHK01_005322"
     CDS             <1..648
                     /locus_tag="ACHK01_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8100057.1"
                     /translation="QLAQQNLVAWMIDVIRHAISGTQERELSLAELSWWAACNQVVDA
                     LPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQRTKNIALPLHVHQQQNPPQ
                     KKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQ
                     GGVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIELIFRFLDHAFATGVLG"
     gene            661..1035
                     /locus_tag="ACHK01_005323"
     CDS             661..1035
                     /locus_tag="ACHK01_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8100058.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            1032..>1675
                     /locus_tag="ACHK01_005324"
     CDS             1032..>1675
                     /locus_tag="ACHK01_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8100059.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGG"
BASE COUNT          443 a          371 c          488 g          373 t
ORIGIN      
        1 caacttgctc agcagaatct ggttgcctgg atgattgatg tcatccgtca cgcaataagc
       61 ggtacgcagg agagggagtt atcgctggcc gaattatcct ggtgggcggc ctgcaatcag
      121 gtggtggatg cactacctga ggcagtagcg cgtcgttcgc tgggattacc agcggaaaaa
      181 atccgctccg tataccgtga gagtgacatc gtaccgggag aacagacagc catcagcata
      241 ctgaagcagc gcacaaaaaa tattgcgctg ccacttcacg tccaccagca acaaaatcca
      301 ccacagaaaa aaacggttgt cagtatcgcc gttgatccgg agtctcctga atcgttcatg
      361 aggcggccta aacgtcgccg ttgggttaat gagaaataca cgcgctgggt aaagacacag
      421 ccgtgtgcgt gttgtggtaa gccagctgac gatccgcatc acctgattgg tcatggtcag
      481 gggggagtgg gaacaaaggc ccacgatatt ttcacgctac cgttgtgccg ggagcaccac
      541 aacgaacttc atgcagaccc gctgaagttt gagaaaaagt acggctctca gattgagtta
      601 atttttcgtt ttcttgatca cgcctttgcg actggcgtgc tcgggtaaaa gaggtgactg
      661 atgctcatag atttggtttt accttacccg ccgacggtga acacttactg gcgacgccgt
      721 ggcagcacat attttgtatc aaaagccggg aagcgttatc gccgggcagt ggcgcttatt
      781 gttcgccagc agcgactgaa attaagcctg tccggacggc tggcaataaa aattattgca
      841 gagccaccgg ataagcgccg ccgtgacctg gacaatattc tgaaagcacc gctggatgcg
      901 ctgacgcacg cggggttgtt aatggacgat gagcagtttg atgaaatcaa tattgtacgt
      961 ggaaagccag tatctggtgg acgtctgggg gtgaagattt accccataat gcttgaaggg
     1021 caggtcaaaa aatgaaactg gaagatttac cgaaatacta ctccccaaaa tcccctggcc
     1081 tgaccgatgc atcggcctca acgtcaaaag atgcgctgag tatcactgat gtgatggccg
     1141 cgcagggcat gacacagaat cgggctgaga tggggttttc tgcgttcctt gggaaaatgg
     1201 gcattagtat gaatgacaga gagcgggcaa cagaattgct gacagaatat gcactcagtc
     1261 ggtgtgatcg cgtggcggcg ttaagaaaac tcccggcaga aataaaaccg gcagtgatgc
     1321 gtattatggc ttcgtatgcg tttgaagatt atgcccgtag cgcggcgagc aaaaaacagt
     1381 gcccctgctg tcacggaaaa aaatttattg aaagcgaggt ttttacaaac aagatccagt
     1441 atccggatgg taagccgcca gtgtgggcaa agtgcacaaa aggcgtgtat ccgtcttact
     1501 gggaggaatg gaaaaaagtc agggaggtgg taaaagttgc ctgtccggag tgtggaggga
     1561 agggagaggt atccactgcc tgtaaagatt gtcgtgggcg cggtgttgcc attcatcgtg
     1621 aagagtcgga aaaacagggt gtgccggttt tcagaaactg tcagcgttgt ggtgg
//
LOCUS       DAZGCS010000315         1619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGCS010000315 DAZGCS010000000
VERSION     DAZGCS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.322"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..62)
                     /locus_tag="ACHK01_005325"
     CDS             complement(<1..62)
                     /locus_tag="ACHK01_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HFO8100060.1"
                     /translation="MSIDALRWAKKVKTGSSSSKS"
     gene            complement(34..585)
                     /locus_tag="ACHK01_005326"
     CDS             complement(34..585)
                     /locus_tag="ACHK01_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8100061.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWVLKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPSVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQISARGSYEYRCTTMG"
     gene            complement(569..796)
                     /locus_tag="ACHK01_005327"
     CDS             complement(569..796)
                     /locus_tag="ACHK01_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8100062.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHS"
     gene            874..1281
                     /locus_tag="ACHK01_005328"
     CDS             874..1281
                     /locus_tag="ACHK01_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100063.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSTLDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            1474..>1619
                     /locus_tag="ACHK01_005329"
     CDS             1474..>1619
                     /locus_tag="ACHK01_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8100064.1"
                     /translation="MDTLNLGNNESLVCGVFSNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNS"
BASE COUNT          441 a          425 c          328 g          425 t
ORIGIN      
        1 gacttactgg atgaactgcc ggttttcacc tttttagccc atcgtagtgc atcgatactc
       61 atacgaaccc ctggcagata tttgtttatc tgcaaagtaa tattgatact gctgacgata
      121 cgcatgcttg aaagcaatag ctttttctat aagctcgtca gtctcacgtt ccacaacaga
      181 tggatccgca aaaagcagtc cggactccac cacatcgcca tattctttgt ttaacccggc
      241 gatcatgtac gtgatgcttt ttccgtcact aatttcacga tacaacctga aatcattaat
      301 ccggatagcc tccataattg caggcactag cgccgtgaac ttttcacgct tatccctggt
      361 gtcgatagcc ttccagcgtt cgaatatctt cactcgatta acgccaagcg ctcgctgatc
      421 aaccgcgcca ccttcatctg tgacacgctg aacatcgatg ttcgggcgct ctttcaaaac
      481 ccagaatgct tcagtgatta atatcgtcgc ctgctcctgt gtcattcctg gtcgacatat
      541 ccaggcatcc agagcctcac gagcctgttc aggagtgatt ttcattgttc aaccgccccg
      601 cccgctttgc cttacgatat tcgtcataaa ctttggggtc gtactgaagt tccccgccgg
      661 atgcctcttg caggcgcatc gcgcgacctt caggaactag ctcttgccat tgagaaacag
      721 cagatgggtc aacgccagca gctttcgcta ctttggcttt cgtaccgtaa aaattaatta
      781 cgtctgattt aaacatcgca cctccaatat tgagttttct caatgctaat cactcaagga
      841 atctcaagtc aagggttatt aagatatcta aatatgaacg agaaaacttt aggtcaacgc
      901 attagagaaa gacgtaagca ggtaggctta agtcaaaacg gtttaagcaa agctgctggc
      961 gtatctggct catcaatttc attatgggaa agtgaccata cagccccgcg cgggcaaaat
     1021 ttgcatcgcc tcgccgaggt attgcaatgt tcaccaacct ggatactgtt tggcgacgag
     1081 gacaaaacac cagctccccc tgtttcactc gatagtaccc tagacttatc ggaagatgag
     1141 ttagagatgc tgcgtttgta tcgcgcactt cctaaatcag agcaacaagc acaactcagc
     1201 gaactccgcg cccgcgttga gaattttaat cgcctgttca ccgagctatt agaggctcgc
     1261 aaacgcaaca agcatcaata atcatccctt cacaaaattt taaagcctta catttcaatg
     1321 tattggcttt atcttgcgcc aatacttgag ttttctcatc aaaaaacctt gacgaaaaat
     1381 aatgagaaaa ctaaattatc tccatcaaca caccgcacgg tgttctccgc aaacagttcc
     1441 gctaccccgg cgttaagggg aaatgaggtc aacatggata cgctcaatct tggcaacaac
     1501 gaatctctgg tatgcggagt attctccaac ctggacggca cgtttaccgc gatgacgtat
     1561 accagaagca aaacgtttaa aacggaagct ggcgcgcgtc gctggttagc aagaaactc
//
LOCUS       DAZGCS010000316         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGCS010000316 DAZGCS010000000
VERSION     DAZGCS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.323"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(144..1058)
                     /locus_tag="ACHK01_005330"
     CDS             complement(144..1058)
                     /locus_tag="ACHK01_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100065.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            complement(1117..>1615)
                     /locus_tag="ACHK01_005331"
     CDS             complement(1117..>1615)
                     /locus_tag="ACHK01_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100066.1"
                     /translation="RLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTKEA
                     NVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEGTA
                     ENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALINV
                     IYTWQ"
BASE COUNT          448 a          363 c          337 g          467 t
ORIGIN      
        1 aacatatttt tcaatataaa aaataagact ctggcttcaa ttgtgcgcgg attttcttac
       61 aggtgtagga taatagcctt cacaaaataa ttatccgcgc aattttcgtg aatggagcgc
      121 atagactaag atcaaaatca caattattca tagataaaag tgacaccaat gactgactgg
      181 acggttccgg ctgcaacctt gtttcctgtt tgtccatagg ttgcgcttaa acccagaggt
      241 actttgcttt tatttactgt ccccagggag acattttctc ccgtagccag tatttttcca
      301 ttacgcatca gagagacacc cactcccgat gcttttgttg catcaggtgc tgtgttagca
      361 aacacttgtc ggctactgtc agtggtggca cccgaaagat aaaatgagag cttttgttcg
      421 cttgagcaat atacgccaag gggtatttct gcgcttccag gaaagtccgg gagatccaca
      481 gtgacattac gactatcaac ggtgcagccg cctgttggca taacaacatt gttatttgaa
      541 ataatattcc acgtaaaatt acgcggattt ccgcttccta atgttgcaat tttatacata
      601 tgaatacgag caatgacttc ccccgctttt atgacaactc caccggccgc cccaactggg
      661 gtaatataga gttttaacgg taatggcatc ggtgtcttat caccaatatc caatacgttg
      721 gtatttgtcg tcaacgggaa ggggtaggtc acattattcc agtacagcga tcctttatat
      781 gactgtaggg agcctgcgaa agctgagcct tgtaccaggt ttatatgatc agtgtcgtac
      841 cagccgccgt aatcattcca gcaacttata tgctgagaca agtctacaac cagattctgg
      901 cccggctgta ttacagggtc gagattaaca taaacagagg ttgtacctgc tccaatactt
      961 gacccgccat cgacattaca actgaaggca aaaactttgc ctgccattaa tagaagataa
     1021 ataccgaaca ggacctttat actgattgtt ttacccatga tatatcctaa ggttaaaaat
     1081 tgatttaaaa agaaggctaa gaaaatcgaa tcgacgttat tgccaggtgt agatcacatt
     1141 gattagcgcc tcgatcgttc cctggcttgc gtttccattc accgtgatag ctcttgcctt
     1201 aagtggaaac tgtgcattac gagtgatctc atcaacaata accgttttgc tatcgccatt
     1261 ttttaacgca gcatcctggt catccctcag ctctatctga atattttcgg cagtaccttc
     1321 atttttgtaa taacccgtat tgtcagttga acctgtcacg attgccgtca ctgcacttgt
     1381 ttcaaccgga caatcggtta atgacaaagt aatattgtgc cagccagatg cagaaccagg
     1441 ttgttgcaga ttgcgcgtat aaagatcccc gagattaacg ttagcttctt tggtttgaat
     1501 agtgcagggt ttagcgacta cccgaccatt aacagtgata gtgacatcgg cggcctggag
     1561 agttgctgtg aataacgcgc aaaacgtagc taacaggaac ctcttgtgaa gtctt
//
LOCUS       DAZGCS010000317         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGCS010000317 DAZGCS010000000
VERSION     DAZGCS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.324"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..596
                     /locus_tag="ACHK01_005332"
                     /pseudo
     CDS             1..596
                     /locus_tag="ACHK01_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            858..>1615
                     /locus_tag="ACHK01_005333"
     CDS             858..>1615
                     /locus_tag="ACHK01_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8100067.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNE"
BASE COUNT          547 a          271 c          306 g          491 t
ORIGIN      
        1 gtcatcgttc tggctagaaa tcatgaaatc aattttaatg gtaactatac tgcatgacta
       61 gaaaattata aactctctgc cggtgatacg tataacttaa ttaaaacgct cgatgtttgt
      121 cattatgact cagaaaggaa agttttaacc agtcatcacg gaataataag agatgaagaa
      181 aaaaatgtta ttgtctcggt gctttgcagg ttccatttaa tcagatgaaa aatcctactg
      241 atcctgagga attagccaat atttttaata aaaaacataa agaacacatg gatgatcctc
      301 tattccatct aatccgaagc aatactttga aacccacacc tgtatatgct aattattttg
      361 ataacactac tgatttcaga ccagcacgta aacgtatctt catatatggg gagacactca
      421 aaggtgaaga ccccagcaaa tacattagac aaaaatatgg gcatcatggg gtcggggtag
      481 atcagaatca acaatttgat aatggtatta tggggctaaa ttccctgaag gaagcacgtg
      541 ataaaaataa taaaataatt tattcctcag ggcttagttg ttttcaatct cattaaaact
      601 ccatgaatac ggtggtatgt ctttttaatc ctgccagtat ttcataggtt ttcttaatcg
      661 attgtagagg catgttctga tgacgcctct acaatcttta gggactttct ctgctatgag
      721 ataagaaatt tcatattatg ctttaaggct gaatgatttg tgcaaaatat aattattttt
      781 ccatcgaaca tgaggtgtat gtcataagat taaatgttaa ctatgcattc ctcgcggtga
      841 attatatgag gtaaaagatg aaaattggaa ctgtggcagg cactaacgac agtacaacga
      901 caatcgcaac aaatgatatg gtgcaagagc atgttactaa cttcactaaa gaactgtttg
      961 ggtatattgc aaatggtata ggtgatgata tatcaagtat tgccaggaca atgctcggtg
     1021 aagttgtgga aaaaattgat gattggcaaa tagaacgttt ccaacaatct attcgagatg
     1081 acaaaatatc atttaccata caaacgaatc attcagaaaa atatagtatg ctatcggaca
     1141 tgcgcgctca tattctacgt agggataata attatcagtt tattgtgaca attaatagta
     1201 aaaattacgg ctgctatctt gataataccg atattaattg gtgcagcatt gtttatttac
     1261 ttaataatat gacagtaaat gatgatgcta atgatgttgc agttactgag tcgtacaaac
     1321 ccgtctggga ctggaaaata tcgcaattca atgtatctga tattaaattc gaaacaatga
     1381 taaaccccca gttcgctgac agaacctatt tctccaattg ttcacctgtt gatcccacaa
     1441 gtaccagacc aacctatttt ggcgataccg atggctcagt cggtgcagtt ttgtacgcgc
     1501 tttttgcaac gggacatttg agaatcatgg ctgaaggaga aaatttcctt agccagttgc
     1561 ttaatataga agatgaagtt ctcaacgtat tgctaaggga gaactttaat gagca
//
LOCUS       DAZGCS010000318         1614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGCS010000318 DAZGCS010000000
VERSION     DAZGCS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.325"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..908
                     /locus_tag="ACHK01_005334"
     CDS             33..908
                     /locus_tag="ACHK01_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO8100068.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVTTGKKPYDILFLHEATKQDFDKKKTEFTFPGANRSY
                     LQSSNTDVAAAAAISIAATEMKTILPKDLTPEKYNKIYLPGDGSAGLPLLKCGDEFLS
                     PTDIVNRLVEHNLHEVEDIRLTSCHSANITKNNDFSPEEIEKSTKTNSGWLASALFGQ
                     KRSLAEHVYAEFEGRGINVSVSGYHGTGVFYVPEHGKPTTHLRSTTVPATPEQTVRRS
                     DYRVSLGRTQPTDID"
     gene            complement(1148..1321)
                     /locus_tag="ACHK01_005335"
                     /pseudo
     CDS             complement(1148..1321)
                     /locus_tag="ACHK01_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          513 a          371 c          298 g          432 t
ORIGIN      
        1 ctcacattta aacaaactaa gaaggaggtt gtatgtttag tataagatcc cagctaccta
       61 taagcgccag cgtatcaatt ccggcagaac aatctcaatc catctccaca attcctggag
      121 ggagaaaaat agagacagtg ccagagaaag aaggattgct tgtgttttta ggaacaaaat
      181 ccgtaggtga atatgctctt aatattcttg gtcaaaatgt ttcaagagtc acaacaggga
      241 aaaaacctta tgatatatta ttcctgcacg aagctacaaa acaagatttt gataaaaaga
      301 aaacagagtt tacctttcct ggtgcaaaca gaagttatct acaatcaagt aacaccgatg
      361 ttgctgccgc agcagctata agtattgcgg caacagagat gaaaactatc ctaccaaagg
      421 atttaacacc agaaaaatac aataaaattt atctgccagg ggatggttct gctggcctac
      481 ctcttcttaa gtgtggagat gaatttttat caccaactga tatcgtcaat cgtctggtag
      541 aacataatct tcatgaagta gaagacatca gattaacatc ctgtcactca gccaacataa
      601 caaaaaacaa tgatttctct cctgaagaaa tagaaaaatc aacaaaaacc aacagcggct
      661 ggttggctag tgcattattt ggtcaaaagc gttcattagc agaacacgtc tatgccgagt
      721 ttgaaggccg tggaattaac gtttctgtat ccggttatca tggcactggt gttttttatg
      781 ttccagagca tggcaaacca acaacgcatc tacgctccac aacagtgcct gcaacacctg
      841 aacagacggt aagaagaagt gactacagag tctcccttgg cagaactcaa cccacagata
      901 ttgactaatt gagtctgcat ataggtttgt gtcattacct gatgcgctac gcttatcaga
      961 cctatattag atcctgcaat atgtttaatt tgtaggcaga atagagcgtt cacaccacat
     1021 caggtataaa caaagtacag cttgccaaaa acctgaataa ccccgaattt acggggttat
     1081 tccgcttaaa catgcccata acatcacttc tgatttgtat tgcttacgac ggatacaatt
     1141 tctctgctca gctacgaata tcatcatact cgcgcgtttt atcgaactcg tgtttcgcaa
     1201 acggacatag cgggataatc ttccgcttct cccggcgcat tttttccacc accttcgcca
     1261 ccagccgttt accaatcccc tgccctttca ggctttcatc aacatcggta tgttcgataa
     1321 tggtcagtta tcagggataa ccctgtgagt acaacgcaat ctgttgctac tgggtagact
     1381 ggtctgctga tcacactcac agatttattt cacatatatg aactcatgta tttgatctct
     1441 ctcttatata tttaatacca atcagacagt cttatcccac cagtgatacc tgtccctgcc
     1501 cacaaggcac gggctgcccc tgtagtggca cactaaattt ggccacctga tgaaaggtga
     1561 tattctcacc acaacacaaa acaggtgact taatgaacaa gaaaaccaaa cgaa
//
LOCUS       DAZGCS010000319         1614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGCS010000319 DAZGCS010000000
VERSION     DAZGCS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.326"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..767
                     /locus_tag="ACHK01_005336"
     CDS             6..767
                     /locus_tag="ACHK01_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8100069.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIAQEGAKTCEEI
                     AGKFSVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTENDGKA
                     FPQPACVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            764..943
                     /locus_tag="ACHK01_005337"
                     /pseudo
     CDS             764..943
                     /locus_tag="ACHK01_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            1364..>1614
                     /locus_tag="ACHK01_005338"
     CDS             1364..>1614
                     /locus_tag="ACHK01_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8100070.1"
                     /translation="MTTQVTVKNLSPVTYNQIPVITTELLARLYGTEAIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELKNLRLVLNESQNEVKISPKTRT"
BASE COUNT          430 a          359 c          465 g          360 t
ORIGIN      
        1 ttttaatgga aaccgtattg catgcactga aagcgatggg taaagccaat tctgttgaac
       61 tggcggcacg aattgatatc agccgtgaag aagttctcaa cgaactgtgg gagctcaaaa
      121 aaaatggcgt tgttgataaa acgggtcaca cctggtttct ggctgtcgaa ggtgaatccc
      181 gggtaaccga agagcggcca gtaaaatctg aaacacagga tatgctgacc gaagaggtcg
      241 ctccaaaagt tagcgctaac atgatgattg agtttatcgc tcaggagggg gctaaaacct
      301 gtgaagaaat agcgggtaag ttcagtgtca gtactcgcaa ggttgcttcc acgctggcgg
      361 tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtagattt cgttactgca
      421 tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa aatgatggta
      481 aggcctttcc tcagccagca tgtgttgcgt taccagtccg ggaagcggaa acacaggaag
      541 aaataaaaac ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc accagaaagc
      601 atccggatgg gctgatttta ccatcgctgc atgtggctaa ccgcgagctg cgccgggcaa
      661 aaggtcaggt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagtgcc
      721 gggatattct ccgggatatt accgccacca gagaacagca gcggtgagtg ggtggaagac
      781 gtggtgccgg gctgaaattc tgatactccg gcagtgcgcg ggaacgatga ctgtcgaaag
      841 tatcggcagt ctgattggtc gtagcgaggc agcggtcaga gcgaaagccc gggataaggg
      901 aataagcctg atgctgcagg gtgattttcg cctgtcagcc aaattaccgt ataaagcgat
      961 ggactgcctg aagcgaaacg gactgattgg cgctgaggat agtttacgat gacctggcct
     1021 gaggcattcg taacggcagg agttgcaatg gcggtagcgc tggtggttta ttcgatttgc
     1081 cgctggggtt gacgtaatta ctaatccggg gctatattca ccgcacggtc gagttgaccg
     1141 tcgggattga gaccccggat attcaaccgc gacagacaca cgccgcgagc gtgtttttta
     1201 ttgtcgtatg cgcacgcaca tctgaattat ggtgaggcgt atggggcagc cgaaaggctg
     1261 gccggttggt tgatccggta gtctcaaccc tgtacgtctc gccacctgat gattgagacc
     1321 tgaaggtggt gatagttaaa ttcatcaacc agagggcgtt atcatgacga ctcaagttac
     1381 tgtcaaaaac ctttcccctg tcacttataa ccagattcct gtaatcacaa ctgagttact
     1441 cgcccgcctt tacggaacag aagcaatccg tatacagcag aatcaccatg aaaataaatc
     1501 tcgatttatt gagggaaaac acttctttaa agctgttggt gatgagctca aaaatttgcg
     1561 actagtttta aacgagtcgc aaaatgaggt gaaaatttct cccaaaaccc gcac
//
LOCUS       DAZGCS010000320         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGCS010000320 DAZGCS010000000
VERSION     DAZGCS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.327"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..230)
                     /locus_tag="ACHK01_005339"
     CDS             complement(<1..230)
                     /locus_tag="ACHK01_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8100071.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGR"
     gene            complement(451..861)
                     /locus_tag="ACHK01_005340"
     CDS             complement(451..861)
                     /locus_tag="ACHK01_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100072.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(877..1560)
                     /locus_tag="ACHK01_005341"
     CDS             complement(877..1560)
                     /locus_tag="ACHK01_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100073.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
BASE COUNT          412 a          364 c          375 g          462 t
ORIGIN      
        1 cggcccgggt cgcgcacacg ggtctggcag gcgaagaacg gctgaaagcc ttcccgctgc
       61 agattttcca ggacggtaat ggtgggaatg tacgcatacc gttcactgcg ggaggtgtgc
      121 cggtcttccc caaaaatact gggtacatgg cgtatcagtt cttcatgtgt cagcggacgg
      181 tcacggcgta tctggtttgc agcataacca aaacgacttg ctaatcgcat actttgctcc
      241 ttatcggtgg ttaagattta ctggtgcaat aaatgaaaaa agccgcgtct cccggagaag
      301 acgcggcctg acgggtgaaa tgaataaggt ttattgtctg agaggccttt cactggcgag
      361 ccacgcatta agcggctttc cggtaacaat ggctggggct gtatttacct ttgatattaa
      421 taatgtgcag agaaacgcat ggtttactcc ttagatttta agtttgtcct gggctacaac
      481 gacaggtttt ccggatgtgt cgatgactcc taccgtcggt acgccatttt tgatttcgaa
      541 gaacggtttg ttgctacctg agcacagttg attgtccccg tattcaatca tttgtgcttt
      601 cccggttttc aggttaacta ccgagacagt gtagtcgtag caccaggcac caccacgttc
      661 aagcacgaaa acatagctgt tatctttata gttgaactga ttctttgtct ggcaattcat
      721 accgtcaatt tcggtacagg aaaacgcttc tgtagtatta acgtatttga tgttatttgg
      781 cagttttact tgtttcgcag tagcgaagac agagaacaga atagttacag caacgagtaa
      841 agttttgata aaagttttca tgatagactc cttaaattag ttaaccgcag ctacgtagtt
      901 tttcaccaga tacgcgaatt catgcacgcc aatttcaccg aaccaaacca ggtaggccgg
      961 gtcatctaaa ccaaagtaac ggtagccaac aacttcatgg gtttttgggt tatagagtgt
     1021 tacgataaag tcccagccgc aggtattggc tttgcaaccg tcgccctgga accagactgt
     1081 accgttaatg tttactggcg tgatacctcc accagggcca tccatatagc cgcctttacc
     1141 gaacatgccg ctcagtccga ccggttttaa tgctttgtcg atagcggctt tgtaggtagc
     1201 gtcaccttcg tacaactcgt acgttgatgg acacgctcca ggttgttcag cccaggcttc
     1261 cgggttacaa ctaagagctt gttttacggt agccgcttct gcgttaaagt taaagcccgt
     1321 gaacagaacc gccagaaata acataagggt atgtaaacgt ttcattgtaa atattcttcc
     1381 gttgtaaatg gcgctggaaa atttctcatc agtgaaacct tccggaagtt tccccccctt
     1441 gtatgatgta tatcgccagt attttatttc atcccggctg taatttttat attccccggc
     1501 acgtgcaata acgctgacca gagatataag gctcattaat aactttacag gtaatttcat
     1561 aattaaattt ccagtgagtg aattgacagg tgaggggtaa aactttctct cag
//
LOCUS       DAZGCS010000321         1604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGCS010000321 DAZGCS010000000
VERSION     DAZGCS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.328"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..764
                     /locus_tag="ACHK01_005342"
     CDS             6..764
                     /locus_tag="ACHK01_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8100074.1"
                     /translation="MGTVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTENDGKA
                     FPQPAGVALPVREAETQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRA
                     KSDVQKWGRVCAALRELNKHRDILRDITATREQQ"
     gene            824..940
                     /locus_tag="ACHK01_005343"
                     /pseudo
     CDS             824..940
                     /locus_tag="ACHK01_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1357..>1604
                     /locus_tag="ACHK01_005344"
     CDS             1357..>1604
                     /locus_tag="ACHK01_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8100075.1"
                     /translation="MTTQISVETLVAITYNQIPVITTELLAHLYGADVKNIQNNFARN
                     VGRFQIGKHFFKIEGEELRELKHRPSLSGSVKIARNVRS"
BASE COUNT          419 a          344 c          475 g          366 t
ORIGIN      
        1 ttttaatggg aaccgtattg catgcactga aagcgatggg taaagccaat tctgttgaac
       61 tggcggcgcg gcttgatatc agccgtgaag aagttctcaa cgaactgtgg gaactcaaaa
      121 aaaatggcgt tgttgataaa acgggtcaca cctggtttct ggctgtcgaa ggtgaatccg
      181 gggtaaccga agggcaggca ctacaacctg aagcgccgga tgtggtaacc gaagaggtcg
      241 ctccaaaagt tagcgctgac atgatgattg agtttatcgc tcaggagggg gctaaaacct
      301 gtgaagaaat agcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc acgctggcgg
      361 tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtagattt cgttactgca
      421 tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa aatgatggta
      481 aagcctttcc tcagccagca ggtgttgcgt taccagtccg ggaagcggaa acacaggaag
      541 aaatgaaaac agaaatcgtg gaagacattg tgaagttaca gccatcggtc accgaaacga
      601 aagcagatga cctgattcta ccatcgctgc atgtggctaa ccgagagctg cgccgggcga
      661 aaagtgatgt gcagaagtgg gggcgagtct gtgccgctct gcgggaactg aacaaacaca
      721 gggatattct ccgggatatt accgccacca gagagcagca gtgagtggct ggaagacgtg
      781 gtgccgggct gaaatcctga tactccggta gtgtgcggga acgatgacag tcgaaagtat
      841 cggcagtctg attggtcgta gcgaggcagc ggtcagagcg aaagcccggg ataagggaat
      901 aagcctgatg ctgcgggggg attttcgcct gtcagccaaa ttaccgtata aagcgatgga
      961 ctgcctgaag cgaaacggac tgattggcgc tgaggatagt ttacgatgac ctggcctgag
     1021 gcattcgtaa cggtaggagt tgcaatggcg gtagcgctgg tggtgtattc gatttgccgc
     1081 tggggttgac gtaattacta atccggggct atattcacct cacggtcgag ttgaccgtcg
     1141 ggattgagac cccggataga aaccgcgaca gacacacgcc gcgagcgtgt tttttattgt
     1201 cgtatgcacg cgcacatctg aattatggtg gggcgtatag gggagctgaa aagctcgccg
     1261 gttggtttcc cggtagtctc aaccctgtac gtctcaccac ccgacgattg agacctgacg
     1321 gtggtgatag tttagaaacc accctagggt gtcgttatga ctactcaaat ctctgttgaa
     1381 actcttgttg ctattactta caaccaaatc cctgtaatta ccactgagtt gttggcgcat
     1441 ctttatggtg cagacgttaa gaatattcaa aacaactttg caaggaatgt agggcgcttt
     1501 cagattggaa aacacttttt taagatcgaa ggtgaagagt tacgtgaatt gaagcacaga
     1561 ccatctttaa gtgggtctgt gaaaatcgcc cgtaacgttc gttc
//
LOCUS       DAZGCS010000322         1597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGCS010000322 DAZGCS010000000
VERSION     DAZGCS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.329"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..711)
                     /locus_tag="ACHK01_005345"
     CDS             complement(<1..711)
                     /locus_tag="ACHK01_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100076.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESC"
     gene            complement(733..1272)
                     /gene="yhaB"
                     /locus_tag="ACHK01_005346"
     CDS             complement(733..1272)
                     /gene="yhaB"
                     /locus_tag="ACHK01_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO8100077.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1528..>1597)
                     /locus_tag="ACHK01_005347"
     CDS             complement(1528..>1597)
                     /locus_tag="ACHK01_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO8100078.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          497 a          275 c          290 g          535 t
ORIGIN      
        1 acaactttcg acaagcttaa ttttttcttc agaatacata tcgctaatgc ttgttagaac
       61 aggcgttatt tttagtaaat aatcccagga gattgtggtg ttttgttttg aactaacgaa
      121 atttacctga aacatgttgg cattagtaaa atctgtgtca tccaatttaa aaggatttaa
      181 aattgcattg gccatatgtt tatctgtaaa atcataaagt tctaaatcga tattccgtaa
      241 gtccagagag gatagatcaa ctatacctcc agtcccacaa ctaaaaacta agttcaacct
      301 cctgatttca ttgtaaatgt ctttcaatgg gaaattaaca ttgatattgc ctgtggatat
      361 ttcctggttt tttaacggtt cattaattgt aagttcacag gtattgtctg atcgttgaat
      421 aattagggcg ttataaccaa aaaaatccat acgaagagac gaatcattat aaataattac
      481 attttgtaag tacgctaatt ctttttgaaa atcaccagtg ataatgcatt ttccacctgg
      541 ttgtaacgcg ttaagtaaag tgaccatttg aatagaggtc tgttttagtt tttctatacc
      601 tggtgctagt gcttcgaggt tattaactat ggggctttca ggaagatcat tcatttttcg
      661 acggactaaa tcactactaa tatcattacc aattgatgaa acagggaaca ttattttatc
      721 ctttataaat aatcaataaa aatcattatt aaaatgatcg ggtgaaaaaa agagaatttg
      781 ccaacaatct gaagtaatgc tggttttatt aaagtgcgca ggaggtgtta agatggcttc
      841 ttttcgggtg aattttgcac aatacttaga aatcgtctcc ttaatacatt ccagctcttt
      901 tttattgacg gctgagcaag tacattgttc accaaaaggt gcaaaagtgc ctacatctaa
      961 tgtttgtatg atataatcgt catgttctcc tgtagaaata tctatgatga atggactcat
     1021 attttccggt tggattcgga tcatgacaga aggaacatcg ccttgataga aattcaatgc
     1081 agtaattgca gataatactc tccgcagttc attacttata ctgtaaattt tattaacgtc
     1141 attggtggag acaatttctg caaactttct ttttaccgtc caataatcaa tatttctatt
     1201 gtgattgtta accactgcgt gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg
     1261 gaagcctttc attgatgcct gccttgtcgt catgacaaac aataatagat gcttattttc
     1321 ctcgcagggt tctttcgcac tatcaaaaaa cgctcattta cccggcgtta cagtaaacga
     1381 agcaacattc tgtggcctac gacgatcaaa agctctgtga aaatttccct tgttgtcttt
     1441 gtctgacctt atcgcggcga tcagcaatta ttcgttttgt gatcctcaac acaaaatgct
     1501 attcgtcgcc tatgagggga gaagaattta tggaaaacga ttgcattcac aaaatgacgt
     1561 cctgtcagaa gagaaaaagt tgacgttaat caattgt
//
LOCUS       DAZGCS010000323         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGCS010000323 DAZGCS010000000
VERSION     DAZGCS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.330"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1594)
                     /locus_tag="ACHK01_005348"
     CDS             complement(<1..>1594)
                     /locus_tag="ACHK01_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8100079.1"
                     /translation="IEICDDDYAGISIGGRVLAVSSQTRTLTLDREITLPSSGTTLIS
                     LVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAAN
                     AWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIT
                     DIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAE
                     GYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT          307 a          494 c          412 g          381 t
ORIGIN      
        1 tgctcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca atctggttgg
       61 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtccctg
      121 agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtctgatg
      181 tcagggaaaa taccggaggg ctgccaccgc tggtaatggt gggggctgtc aggcgcttca
      241 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
      301 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagcttgc
      361 cttcctccgt gtcctccatg ctgagaccaa tacccgcgac ataatgtttg ccgtctttgg
      421 tctgctcaat tttgacgccc cacatggcat tccatttatc gttagcgtcc ttccactctt
      481 tcgaaaactc atccagtctg ctggcgttat cctccgtcag atcaactttt tccagcagct
      541 ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat
      601 catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg ttcacactgc
      661 ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct atccagtaca
      721 gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta atccgctttt
      781 ccgagaacca gaactcaaac tgtaccgtcg gatcataaac ggcaagatgc ggcgttgcgg
      841 ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc
      901 ggaacgatac cgacgccgga tcgccctgct gcccccaggc atttgccgcc cggactgtca
      961 gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccgggccg
     1021 tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     1081 cgcccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg
     1141 cggtgacttc ggcagtcagg tgctgcaccg cgggcggcgt gacgccattc accgtgccgc
     1201 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttttcg ggtacatgct
     1261 gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg cagcggaaca
     1321 ggcgctggcg caacgtcggc aacttcagcc cccatacgct gtattcagca acgccgtcag
     1381 gaacacggct cactttcacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     1441 gattaccctg cccgtcaacc aggcttatca gcgtggtgcc ggaggatggc agcgtgattt
     1501 cacggtcgag cgtcagcgtc cgggtctggc tgctcaccgc cagcacgcga ccaccgatgc
     1561 tgataccggc atagtcatca tcgcagattt caat
//
LOCUS       DAZGCS010000324         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGCS010000324 DAZGCS010000000
VERSION     DAZGCS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.331"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            136..906
                     /locus_tag="ACHK01_005349"
     CDS             136..906
                     /locus_tag="ACHK01_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8100080.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            922..1335
                     /locus_tag="ACHK01_005350"
     CDS             922..1335
                     /locus_tag="ACHK01_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO8100081.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT          462 a          302 c          456 g          367 t
ORIGIN      
        1 acacttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg atgagtgacg
       61 tttaactggg atagctggcg tccaaatgtc agcaatatga gggttgtgaa gtaattttgt
      121 ccggaggaaa ttttaatgga aaccgtatct gacgcactga aagcactgaa aaaagcctct
      181 tcacatgtgg tggcagctcg ccttggaatc agtcgtgaag aggctgtcaa cgagctgtgg
      241 gaactcaaaa taaatggcgt cgttgataaa actggtcaca cctggtttct ggctggcgaa
      301 ggtgaatccc gggtaaccga agagcggcca gtaaaatctg aagcacagga tatgctgacc
      361 ggggaggtcg aacaaaaagt taccgcagac atgatgattg agtttatcgg tcaggatggg
      421 gctaaaacgt gtgaggaact ggcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc
      481 acgctggcgg tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt
      541 cgttactgca tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa
      601 agtgatggta aggcctttcc tcagccagca ggtgctgcgt taccagtccg ggaagccgca
      661 acacaggaag aaattaaaac agaaactgtg gcggacattg tgcagccgtt gccatcgttt
      721 accgaaacgc aagcagatga gctgattttt ccgtcccttc gcagggcaaa cctggcgctg
      781 cgcagggcga aaagtgatgt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg
      841 aacaagcacc gggatattgt tcgacagatt actgattctt cccgccgtgt tgtatcggaa
      901 aagtgattgc cggaggcgct tatggcaaaa gtatttacac aggaagagcg ggaaaaaatt
      961 aaagggcagg ttgttgagct agtacgccgg agtgggcgcg agacgttacg gcaactggaa
     1021 gccaagacag gtgcgacaag atatctgatg agcgttcttg ccagagagct ggttgccagt
     1081 ggcgatgtat acaactctgg ttacgggtta ttcccatctg aacaggctcg taaggactgg
     1141 caaaacgccc gcaaaaaact atcgagggca aaactgaaga aaccggttgt ggttgatcct
     1201 gaccttatct ggtcattacc agacggagaa atacgccgct atgacaggca attgaacata
     1261 atttgtagtg agtgccgtaa cagtgaagtg atgcagcgag ttttgatatt ttacacagga
     1321 gtaatgatgg aatagtgaaa ataacatgaa acctttggat tctggtgttt cagtggatgg
     1381 gaaaagagca gatacataga aaatgaatag caataattca cagtctggat gttgtttgta
     1441 tgcttataaa atgatcctag tatgcattgc cggagttgaa tcagctccaa tgaagaaact
     1501 gagaggagta tttagaagat agctaaatgg atgtaattat tagtatctaa aaaatagtat
     1561 ttttgaaatg ggtctaagaa cacaaca
//
LOCUS       DAZGCS010000325         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGCS010000325 DAZGCS010000000
VERSION     DAZGCS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.332"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..656)
                     /locus_tag="ACHK01_005351"
     CDS             complement(<1..656)
                     /locus_tag="ACHK01_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO8100082.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHK01_005352"
     CDS             complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHK01_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO8100083.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHK01_005353"
     CDS             complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHK01_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO8100084.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1199..1339)
                     /locus_tag="ACHK01_005354"
     CDS             complement(1199..1339)
                     /locus_tag="ACHK01_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO8100085.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          431 a          370 c          359 g          408 t
ORIGIN      
        1 ttgatcatag tggtgtgcga gtcagtacca acgagtgtat ccggataagc aatccattca
       61 ccgtcctgca attcactcca cactgctttg ccgagatatt cgaggttaac ctgatggcaa
      121 atgcctgtgc ctggcggcac gacgctaaac cgactgaacg cttgctttcc ccatttcagg
      181 aacacataac gttcgtggtt gcgctccatt tccaggcgta cgttttcttc aaatgcctca
      241 tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc
      301 gggttaacct ttgcagtatc gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga
      361 tcaacaacgg caggtacgcc ggtaaagtcc tgcatcagca cccttgccgg gcggtaggca
      421 atttcacggt cagcatgggc atttttcagc catcctgcca gcgcgtggat atcctcttcg
      481 gtaaccgagt taccatcctg ccagcgcagc aggttttcga gcaaaacttt gagtgatttg
      541 ggcagacggg tgatatcgcc cagtgattta gcagcaagcg gcaggctgta gtagtggtaa
      601 gttttatctt tggcctgcaa cgtgtcctta ctggcttctc gtagggttga cgacatagct
      661 cctccttaat gacagggttg cgaagccctg attctcttca gggtgaatat taaagataac
      721 acaaacatcg cgtaacgatt tgataacaac ccaaattgat aaaagaggag aaaccttgtg
      781 gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag
      841 gagttcggcc cagaagagca tcgacagaga aaacgcgccc atccaggacc aatatttcat
      901 atttgtgttg atcataggct acacccttaa cggttattat tttgtgttga gataactcaa
      961 gctgttattg ctgcggtgta ttaaaagccc tttaattcat taaaaatggg taataagaat
     1021 gatgataatt attgtttcgg ctgatcatct tcagcaaaca gttctataaa ggcttgctgc
     1081 tgcgatgtta gttgccagtc gtctggaata acgacggaac attcccgttt cgtttctgtt
     1141 tcattgtcac tatgctggca taatggtgac gactccagtt gctcctgacc gaccatagtt
     1201 atccccacac tttccaaatc agcagtgcga caaccaccca gaaaagtgca gaaccaagaa
     1261 aaaccgccag ccaggcttta cgttttaatt caggatcgcg gcggggttcc tgatttccag
     1321 aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg aacgattggt
     1381 gtaattaatc atacgatgag acaaatccta aagaaacttt agtcaaaaat ccagcgaatt
     1441 tacgaatcgt ctccagtgaa taattcaatc ttttgacctg aaatagatat ttgaaaactg
     1501 gagtttgcga ggcgggtaat tagacactac tttacaggta atttacccta taaatatgag
     1561 ggataaaa
//
LOCUS       DAZGCS010000326         1565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGCS010000326 DAZGCS010000000
VERSION     DAZGCS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.333"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..603
                     /locus_tag="ACHK01_005355"
     CDS             <1..603
                     /locus_tag="ACHK01_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100086.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRSTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            631..1092
                     /locus_tag="ACHK01_005356"
     CDS             631..1092
                     /locus_tag="ACHK01_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO8100087.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1112..>1565
                     /locus_tag="ACHK01_005357"
     CDS             1112..>1565
                     /locus_tag="ACHK01_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO8100088.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAA"
BASE COUNT          424 a          401 c          448 g          292 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg atcattatgg ggcgcaccta ccaccaggaa
       61 aaccgcaccc ccggcagcct gccgggaaca aagacgcaga tgaccatccg ctccaaaacg
      121 tacatgggca gcggatttaa tgagctgaag tttgatgatg cgacagggag agaacaggtc
      181 tacatccacg cgcagaagaa catggatacc gaagtgctca acgaccgtac caccaccgta
      241 aaacacgatc accgcgaaac cgtaaaaaat gaccagacgg tcacgatcca ggaaggtaac
      301 cgccttctta cggtggaaaa aggccacaag atcaccggag tactgaaagg gtctttatct
      361 gaggatgtct ttcaggacag aagcacgatt gccggttcgg tgcatgttga cgctgtaaac
      421 aatggtggcg aaggcgacgg tatacaggct tatacggcga ttaaggaaat tttgctggcc
      481 gtggaggaaa gcaaaattgc gctgacgccg gatggcattc agctacaggt cggggaatcg
      541 acggtaatca ggctgtcgaa ggatggcatc accatcgtgg gcggttctgt tttcatcaac
      601 tgattcactc agattatcag ggagtatttt atgcagttca cttttaatga aggccacatt
      661 caactgccgt ctcaatggca ggatcagtca atgcaggtcc tggtgtccac agataacagt
      721 ggcatcaacc tggtcattac ccgcgaagcc gttccacagg gaaccctgac accagagctg
      781 taccaggaaa cgctggcgct gtaccagggc aaacttgatg ggtacaccga acatgcctgc
      841 cgggaaatca ctctggcaga ggccccggcc tggctgctgg attattcctg gaacggtccg
      901 gaagatgagg ggaatcaggg gcggattagc cagatagccg tattccagcg gcgtggagat
      961 acgctgctga ccttcacttt cagcacctcc ttatccctga agaacagtca aaagacgatg
     1021 ctgctggagg tgataaagag cttcaccccg ttgccaccgg agaatgacat ccaaaaggac
     1081 cagccgcgct gacataaacg agggataaca catgagtgaa ggaccaggcg ggccacaggg
     1141 agcgaccgca ggcggtacgc tggcaatgcg aatgctgtca cagcaggcga tggtcgccag
     1201 ccagatgaaa cgggcagcca acgacaaagc cattgcacag atgctggcag caaagaagtc
     1261 cggcccacct gccgccaggc tgggcgatga aattcagcat aaaagttttc tgggggcact
     1321 ggcaggggcc gtgctggggg cgatagtgac catcgcagaa ggttgcctga ttatggccgc
     1381 ctgcgccacc ggcccttatg cgctggttct ggtgcctgcg ctgatgtatg ccagctataa
     1441 ggcgagtgat tatgtggagg agaaacagaa ccagcttgaa tcatggataa acagcttttg
     1501 tgacacggac ggcgccatca ataccggttc tgaaaatgta aacattaacg gaaagcccgc
     1561 tgcaa
//
LOCUS       DAZGCS010000327         1549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGCS010000327 DAZGCS010000000
VERSION     DAZGCS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.334"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..565)
                     /locus_tag="ACHK01_005358"
     CDS             complement(<1..565)
                     /locus_tag="ACHK01_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100089.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLG"
     gene            684..1549
                     /locus_tag="ACHK01_005359"
                     /pseudo
     CDS             684..1549
                     /locus_tag="ACHK01_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          498 a          303 c          269 g          479 t
ORIGIN      
        1 ttccaagttt tggaggtttg ataggcgttt taatttcact gcgcaacgca tctaagtttg
       61 taagatcact gtctataatt ttaagatagt ttaaatctaa cgaaccatca gttttatcaa
      121 gcttagtttt cagcctttta acagcctcca aggaagcatc aagaactggt gtctgattct
      181 tcaaagcttc tgaaaaactt ttttgtgttg attttcgagc ttgttgttga gcagattttc
      241 ttaactcttt ttcacgctta acttgttcta aaagctcttt acggtcatca gaacgtgaac
      301 caaaaaatct atcagcaagt tcagttaaca aattagatat aatagttttc agttctcttg
      361 cagcctggtt tcttatgaat ccctctctcc ccgacttatc tttcagctct ttattactgg
      421 attgagtaat tccaatataa ccaaaaatcc ttctattaga ccaataatat cgccctgcat
      481 tccatgaacg tctttcttct atctggaaga aatcattatc tactcgacca taaggtaata
      541 ctctcaagct atccctaaaa atcattagtc ctgcatactt tttggcctta agatcaaagt
      601 gggaatgttc acgttcagta tgtgatgtat tttgtgaaag gaattcaaac gttcctatct
      661 gaagctcaaa tgggccgacc cctgaatggg aaaacctcca tgcattaaaa gaaaagcgtt
      721 taaatggaat gcctaaaaca acccccaaaa gtgaccaggt tattatgctg caaaatcaat
      781 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttacgatgca gcaaaacact
      841 caaaacatga acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcggt
      901 tacacaatac taatttattt aacccaaaat atcatgaaaa agtcgttatg aattacatgg
      961 aatatctggt aacttgtcag ttggatgaag aacaaatgtc atcgctgctt tatgaaagag
     1021 atgatttaag cgccattgat ttttcattgc tcatgaagac gataaaagct ttttcatttg
     1081 gtggcgatct ccaaaccctt gcatcaaaac cgggttccac aatctcaagc atcccatcaa
     1141 aaagacggat attgattaac attaaacacg attttcctaa caatggcaat attttcaatg
     1201 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
     1261 cgttcagcag gcctttagtt tactgggatg gccaagtatt aaaatcaacg actgaaatta
     1321 aaaattatga tgggtcgacg cgtgttggcg atgaggcgtg gcttaataaa ggttgctcta
     1381 cctatcagca gctctacaat gaattccacg cattatatat agataaagca ggaatatgga
     1441 gcgcatttga gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattctg
     1501 tgaacattaa tcactctcct catgatggac taattataat aaataaagg
//
LOCUS       DAZGCS010000328         1547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGCS010000328 DAZGCS010000000
VERSION     DAZGCS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.335"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..790
                     /locus_tag="ACHK01_005360"
     CDS             47..790
                     /locus_tag="ACHK01_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8100090.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGRNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            787..1365
                     /locus_tag="ACHK01_005361"
     CDS             787..1365
                     /locus_tag="ACHK01_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8100091.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          327 a          402 c          495 g          323 t
ORIGIN      
        1 ggcggtttcc tttccattaa taaactttcg cagtaaatcc cggtttatga cacagactga
       61 atcagcgatt ctggcgcatg cccggcggtg tgcgcctgcg gagtcgtgcg gcttcgtgat
      121 aggcaccccg gagggcgaac ggtaccagcc ctgcgtgaat atctccgcag agccggaggc
      181 gtattttcgt attgcgccgg aagactggct gcaggcagag atgcaggggg agattgtggc
      241 actggtccac agccaccctg gtggtctgcc ctggctgagc gaggccgacc ggcggctgca
      301 gataaagagt gccctgccct ggtggctggt ctgccggggg gaaattcaca aattccgctg
      361 tgtgccgcac ctgaccgggc gtcgttttga gcacggggtg acggactgtt acaccctgtt
      421 ccgggatgca taccatctgg cggggataac gctgccggat tttgtgcgtg aggatgactg
      481 gtggcgcaac ggtcggaacc tttacctgga caatatggcg gcgactggtt tttaccgggt
      541 gcccctgtcc tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc
      601 caatcatgcc gccatatact gcggcaacgg tgaactgctt caccatctgc ctgaacaact
      661 gagtaaacgg gagaggtatt ctgaaaaatg gcaacgacga acgcattctg tctggcgtca
      721 ccgccactgg cacgcatctg ccttcacggg gatttgcaac gatttggccg ccgcctcagc
      781 ctgtacgtga acacggcagc ggaagccatc cgtgccctgt cgctgcagat gccgggattc
      841 cgccgtcaga tgaacgaagg ctggtaccag atacgtattc gcggtgagga cacggcaccg
      901 gaggcggtgt acgcccgtct tcacgaacct ctgggtgagg gggcggtcat ccatattgtg
      961 ccgcgactgg ccggagccgg aaagggcgga ctgcagattg tgctgggggc agcagccatc
     1021 gtgggctctt tcttcaccgc cggcgcaacg atggcgttgt ggggcgcagc cctgagtgcc
     1081 ggagggctga ctgccaccac gatgctgttc tcactgggtg ccagcatgat actgggcggt
     1141 gtggcccaga tgctggcacc gaaggcaaaa acaccggatt accgcgcaac ggataacggt
     1201 aaacagaaca cgtatttttc gtcactggat aacatgattg cccaggggaa cccgatgccg
     1261 gtgccttatg gtgaaatgct ggttggttca cgacggatat cccaggacat cagcacccgt
     1321 gatgagggcg gagacgggaa agtggtggtt atcgggcggg gatgaaaata aaaaaatccc
     1381 gcagagttag cggagctgcg ggagagaacg atgaagatta acgttatgga gttatttttc
     1441 aggcatcaaa aaagtaatgc agcgtcatta ttgcggctac aggcaattgc cggaaatgtg
     1501 aagagtttca gaaattttat tccgtcatga cacaggcacc ctccggg
//
LOCUS       DAZGCS010000329         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGCS010000329 DAZGCS010000000
VERSION     DAZGCS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.336"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1226
                     /locus_tag="ACHK01_005362"
     CDS             <1..1226
                     /locus_tag="ACHK01_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8100092.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKENTLALGVYPE
                     VSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQA
                     VAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKF
                     ISPFEYAKINNPLKQ"
     gene            complement(1343..>1510)
                     /locus_tag="ACHK01_005363"
     CDS             complement(1343..>1510)
                     /locus_tag="ACHK01_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO8100093.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
BASE COUNT          407 a          371 c          361 g          371 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggtt catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa ggagaatact ctggcacttg
      121 gtgtgtatcc agaagtttct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcaga ggggattgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc ttttgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaggta ctcaaaagcc tcgaaacaca cgttttcccc tttatcggca
      361 accgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgctgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat acgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttctcacacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag caaacaggct gtagccatac
      841 tgacagaact tcagacatgg gcaggtgaaa atggtctgat atttacgggt gcacatgacc
      901 cgcgtaaacc aatcagtgaa aatactgtaa ataaagccct gagggtgatg gggtatgaca
      961 caacccagga agtctgtggc catggattcc gggcgatggc gtgcagtgca ttgattgaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gtcagatgag ccatcaggaa cgtaacggtg
     1081 tacgtgctgc ttacattcat aaagcagaac atctggaaga acgccgcttg atgctacaat
     1141 ggtgggccga ttttctggat gcaaacagag aaaaatttat cagtccattt gaatatgcaa
     1201 agattaataa tccattaaaa cagtaatcat cccgggcaaa tgcccgggaa cctactccag
     1261 gattattttc tttgttaaaa aaacagacaa aacggtatta acttcgtaag cgtacagcga
     1321 gggccgtatt gacggggatg tgttattcag ctggcagtgc tatgcgccac ggaagcagtt
     1381 cgctgacccg gttgaccggc cagtctgcta tgacgccaag cacatggcga aggtagcttt
     1441 ctggatccac gtcattcagt ttgcacgtcc cgatcaggct gtacagtagc gctccccgct
     1501 caccaccatg
//
LOCUS       DAZGCS010000330         1478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGCS010000330 DAZGCS010000000
VERSION     DAZGCS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.337"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..628)
                     /locus_tag="ACHK01_005364"
                     /pseudo
     CDS             complement(1..628)
                     /locus_tag="ACHK01_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299329.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            complement(684..878)
                     /locus_tag="ACHK01_005365"
                     /pseudo
     CDS             complement(684..878)
                     /locus_tag="ACHK01_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1228..>1478)
                     /locus_tag="ACHK01_005366"
     CDS             complement(1228..>1478)
                     /locus_tag="ACHK01_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100094.1"
                     /translation="HYFRAEGSSPPQSMATGINKVRCDAGMEEKHVNALDIFTHSYPE
                     IYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT          389 a          314 c          289 g          486 t
ORIGIN      
        1 tttgcggatt tctctatttc atcaggagag aagtctttgt tttttaatat gttggctgag
       61 ttacaggatg ttaatctgat atcatcaatc tcatgaagat tatgttgaac aatgcggtcg
      121 acaatatctg tcggcgataa aaattcatct ccacacttaa gaagtggtag accagcagaa
      181 ccatccccag acagataaat tttttgtatt ttcctggtgt taaatcatct ggcaggatgg
      241 ttttcatctc tgtcgctgta atacttatag ctgcagcagc aacaacatcg ctattatttg
      301 attgtagatg gcttttattt gctccaggat atgtaaactc cattttcctt ttatcaaaat
      361 cttgttttgt agcatcattc aggaataata aatcatacgg ttttttcccc gttgtgactc
      421 ttgaaacatt ttggccaaga atattaagag tatagtcatt aacggatttc attcctaaaa
      481 aaacaagtaa tccttctttc tcttgcgctt tttcgattgt tctccctgct aaagttattg
      541 ggatggattg agattgtttt gtcggaactg atacgctggc gctaatataa agacggcgca
      601 atggccgcca caggttcttt ctgctcatcc ggcgttattt ctacaggtaa taattcaacg
      661 ccagtgtcag aagaggttgt taccggaaga cgccgcgaaa cacgcccttc gttctgccag
      721 agcctgagcc atttgaaaat aacattatca ttgacgccat tttctcgtgc aatctgtgca
      781 acacaagctc caggttgtga tgccagttca accatacgaa gtttgaattc attcgaatac
      841 tttttacgag gttcttttcg ccagtcctgt aattccatac ttagatgtcc gtctgtatca
      901 gatgggcgtc taagttacca attcttgtct aatggctaca tacggcgatc ggtttacgct
      961 tacggacaaa aaatcacctt gcgctaatgc tctgtctcag gtcactaatc acctctaact
     1021 cattgattaa tagtggtgcg aaagattggt gagatatcgt tgcgtaacac ttcctggcag
     1081 aagggacagg gcagactctc cttcaggaga atctgtaatc agtatacaat caatgccagt
     1141 tttttcgcat tgattgtaaa attcatcaat aatgcctgga atgtggatta tattcttctg
     1201 gggcgggctt cttcacttag aacattttca atctttggtt tcagcttcgt gcagaattcg
     1261 agcggcttct tgcgtaacat tatacattgc ctgctcatca ttaccgccag ccccagcaat
     1321 cgttaatgca accccagcat aaatttctgg atagctatgt gtgaatatat ccaaggcatt
     1381 tacatgtttc tcttccattc ctgcatcaca acgaacctta tttattcccg ttgccattga
     1441 ttgtggagga ctggagcctt ctgcacgaaa atagtgat
//
LOCUS       DAZGCS010000331         1470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGCS010000331 DAZGCS010000000
VERSION     DAZGCS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.338"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..1470)
                     /locus_tag="ACHK01_005367"
                     /pseudo
     CDS             complement(63..1470)
                     /locus_tag="ACHK01_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
BASE COUNT          277 a          445 c          401 g          347 t
ORIGIN      
        1 tttttaaaca taaaatgcga attatttagt acaaaaaacc ggcgttacat gatctttccc
       61 tggctccacg tctggtcgtt atcggaccag cggatgatcc ggtccgcagc gtcgtactca
      121 taaaacaggt ggtagtccag ccgcgcatcc gcttcggtca gccgcccccg cgcatcctgt
      181 tcgtaggtcg ccagcgtctg tatgccgtta tccgtgcggt gaatggcttt caggtagccg
      241 ctggcgtggt acgtcagttt aagccggatc ccgtcggtgt gactgacacc cgtgatccgg
      301 tactggctgt cgcgctcaaa cgtgatgcgg ttgctggcgg tgtcgctcat cccgcccagc
      361 atgtacatcc ggtcattggg gctgtgtgcc aggcggtcat cgctggtatc gtcagtcacc
      421 gggtggcgca gacgcattcc cggaaaggcg tcataaaaga agcggctgct cagtgcatcc
      481 ctgtcgaaca gctccagata atcatcgcgg cggcgcagaa tgtactgggc gtgatacggg
      541 ttaaccgtcg tatcactgtc accgtcgaag gcaaatctca gcgtggctcc ctggctgatg
      601 atatccaccc ggtttccctg ttcacgcacc caggcgtatt cgctccagga gtcgctccag
      661 cccacgcccg tcaggccatg cagcggcagt acagaactgt ggaaacgggt gaaggagagg
      721 gggagggtct ggcccagggt gaagtcggta cgttcgtcgc agtacgcgcc ggttaccgga
      781 tcgacaggat ctgttaccca tctgatgact ttattggcaa tactttttaa cgacttacct
      841 gttttcccca gcaacgcgcc gagctttgcg cccagcagct tcccgctttt ccccagcagg
      901 tttcccagtt tgcccagttt cgccagattc cccagttttc ccagcagtcc tgctggcggc
      961 agaagatagc tggctatcgt tattcctccc aggatcattc tctgccatgg cgggaactcc
     1021 ggctggatat ccagataggt gacctgctca cccccaataa agacgtccgg cgaaccttcc
     1081 acgatcgccg cactgcactc cagtttgtcg cccttgcgcg cggcaggctg attgttgata
     1141 aagacgttgc tactgccctg ggctatcctc ggtggtttgt tctccttgtc gcagagcgcc
     1201 gtgtcctcac cggcttccgg gaaatccacc tcttttttga tttcgccacg cccgcccacc
     1261 atggtggcaa actccattaa taccggaccg gcgggaaaca gaagcgatgc gccggcgctg
     1321 acgcgatcca taaaaccggc attgctttcc gtcagggtga taacagcgtt cccccaggcc
     1381 ttcgccagtg gtactgcttt ttccgctgcc gattcaagca ggtcagtggc aatatcaccc
     1441 catgagggtt ccccctgtgg gatttcaggt
//
LOCUS       DAZGCS010000332         1432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGCS010000332 DAZGCS010000000
VERSION     DAZGCS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.339"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..761
                     /locus_tag="ACHK01_005368"
     CDS             <1..761
                     /locus_tag="ACHK01_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO8100095.1"
                     /translation="ARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDE
                     EETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGA
                     SVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDP
                     EIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQL
                     DLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            745..951
                     /locus_tag="ACHK01_005369"
     CDS             745..951
                     /locus_tag="ACHK01_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO8100096.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            948..>1432
                     /locus_tag="ACHK01_005370"
     CDS             948..>1432
                     /locus_tag="ACHK01_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8100097.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSVDA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     L"
BASE COUNT          332 a          322 c          474 g          304 t
ORIGIN      
        1 ctgcccgggt ggtttatctg acggcgggca ttgactcgca gcgaaaccgt tttgagatgt
       61 atgtctgggg atgggctccg ggagaggaag cctttctggt ggataaaatc atcattatgg
      121 ggcgtcctga tgaggaagag acgctgttac gtgtggatgc ggcgatcaac aaaaaatacc
      181 gccatgcgga tggcaccgaa atgactattt cccgtgtctg ctgggacacc ggggggatcg
      241 atggtgaaat tgtttatcag agatcaaaaa aacacggtgt tttccgggtg ctgccggtaa
      301 aaggcgcatc tgtctatggc aagccggtga tcaccatgcc aaaaacccgc aatcagcggg
      361 gcgtgtatct gtgtgaagtg ggaacggata ccgcaaaaga aattctctat gcccgtatga
      421 aagccgatcc cacgcctgcg gatgaagcca cgtcgtatgc catccgtttt cctgatgatc
      481 cggagatttt ttcgcagaca gaggcgcagc aactggtggc ggaagagctg gtggagaagt
      541 gggaaaaagg aaagatgcgt ctgctgtggg ataacaaaaa gcggcgtaac gaagcgctgg
      601 actgcctggt gtatgcctac gcggcattac gtgtgtccgt gcaacgctgg cagcttgatc
      661 tggctgtact ggcaaaatcc cgggaagaag agacgacccg gccaaccctg aaagaactgg
      721 cagcgaagct gtccggagga gtgaatggtt acagtcgctg aactgcaggc gctgcgtcag
      781 gcgcgccttg atttattaac cggtaaacgg gtggtgtctg tccagaaaga tggtcgcaga
      841 attgaatata cggcggcttc tctggatgag cttaaccggg cgatcaatga tgcggagtcg
      901 gtactgggga caacccgacg tcgccgtcgt ccgctgggag tgaggttatg aaacgaacgc
      961 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacgggggcg
     1021 gcgcaggatt tggcgggcaa atggcggagt ggttgccacc ggcgcagagt gtcgatgcgg
     1081 ccctgctgcc cgcgttgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     1141 gaatagcggc taatgcggtg gccctgcata aggatcacat tgtcgggcat atgtttctga
     1201 tcagctaccg tccgaactgg cgctggctgg ggatgcggga gaccgcagca aaaagctttg
     1261 tcgatgaggt ggaggcggcc tggtcggaat acgcagaagg gatgtctggc gagatcgacg
     1321 tggaaggaaa acgcacgttc acggaattta tccgtgaagg tgtgggcgtt catgcgttta
     1381 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta tt
//
LOCUS       DAZGCS010000333         1422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGCS010000333 DAZGCS010000000
VERSION     DAZGCS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.340"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..978
                     /locus_tag="ACHK01_005371"
     CDS             1..978
                     /locus_tag="ACHK01_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8100098.1"
                     /translation="MSAFHNGRILVEPEPKSMRALPSGVVPAVHQPLAEDKSLLPFFS
                     DERVSRAAGGAGALSDWLLRHVKSCQWLHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPPQEKTVVS
                     IAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTK
                     SHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            991..1365
                     /locus_tag="ACHK01_005372"
     CDS             991..1365
                     /locus_tag="ACHK01_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8100099.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            1362..>1422
                     /locus_tag="ACHK01_005373"
     CDS             1362..>1422
                     /locus_tag="ACHK01_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100100.1"
                     /translation="MKLEDLPKYYSPKSPGLTDA"
BASE COUNT          344 a          363 c          396 g          319 t
ORIGIN      
        1 atgtcagcat tccataacgg cagaatactg gtggagccgg aaccaaaaag catgcgagct
       61 ctgccgtccg gggttgtacc tgccgttcac cagccgctgg cggaagataa atcactactg
      121 ccatttttca gcgatgagcg ggtgagccgt gctgcgggtg gcgctggtgc actgtctgac
      181 tggttattac gtcacgtgaa atcctgccag tggctacacg gtgattatca tcacagcgaa
      241 accgtcattc accgttacgg taccggcgca atggtgttgt gctggcactg cgacaaccag
      301 ctgcgtgacc agacatccga atcactcgag caacttgctc atcaaaacct gtcagcatgg
      361 atgattgacg tcatcggtca cgcaataagc ggtacgcagg agcgtgaatt atctctggct
      421 gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga agcggtatta
      481 cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcca tgtaccgtga aagcgacatc
      541 gtaccgggag agcagaccgc caccagcata ctgaaacagc gcacaaaaaa tcttgcgccg
      601 ctgcctcacg cccaccagca acagaaccca ccacaggaaa agacggtggt cagcattgcc
      661 gttgatcctg agtctccgga atctttcatg aaacgaccta aacgtcgccg ctgggttaac
      721 gagaaataca ctcgctgggt gaagacacag ccgtgtgcgt gttgtggtaa gccagccgac
      781 gatccccatc acctgattgg tcatggtcag ggcggaatgg ggacaaaatc tcacgatatt
      841 ttcacgctac cgctgtgtcg ggagcatcac aacgagcttc atgcggatcc gctggcgttc
      901 gaagaaaagc atggttctca ggttgattta atttttcgtt ttcttgatca cgcctttgca
      961 actggcgtgc ttgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
     1021 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttatctc ggaggaggga
     1081 aagcgttatc gccgggctgt ggcgcttatt gttcgccagc agcggctgaa attaagcctg
     1141 tccggaaggc tggcgataaa gattattgcc gagccaccgg ataagcgccg ccgtgacttg
     1201 gacaatattc tgaaagcgcc gctggatgcg ctgacgcatg cggggttgct aatggacgat
     1261 gagcagtttg atgaaatcaa tatcgttcgt gctcagccag tatctggtgg acgtctgggg
     1321 gtgaagattt accccataat gcttgaaggg caggtcaaaa aatgaaactg gaagatttac
     1381 cgaaatacta ctccccaaaa tcccccggcc tgactgatgc at
//
LOCUS       DAZGCS010000334         1412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGCS010000334 DAZGCS010000000
VERSION     DAZGCS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.341"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1266)
                     /gene="wzx"
                     /locus_tag="ACHK01_005374"
     CDS             complement(1..1266)
                     /gene="wzx"
                     /locus_tag="ACHK01_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O103 family O-antigen flippase"
                     /protein_id="HFO8100101.1"
                     /translation="MEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLG
                     QLQNFVAFINGFISSQVSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGV
                     LFSSKISEYLFYQSDLYWLIIIALCALPLNIVNNIFLGVLNGLNEYNRFFLANVLAIS
                     SSLLSMVGLVYFFGLKGALVSASLNNAVAGVWLITIIIKRPWFKFKYWVGHTPRHNIT
                     QMKNYFYMGVIGALTGPISMIVVRTILTNNFSLEDAGYWQAVNRISEAYLAVLTTALT
                     VYYFPKTAAARRYSEYITLLKTGACIVVPLALSMALTIYGLKDFIISILFTADFIRAR
                     ELFLFQNIGDFLRICSWLFATILLAKGYFKINAILEVMFSIIFPVLVKFLIINFGLNA
                     VSIAYSITYALYFIVVCIIYCWHLTKKAL"
     gene            complement(1254..>1412)
                     /locus_tag="ACHK01_005375"
     CDS             complement(1254..>1412)
                     /locus_tag="ACHK01_005375"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO8100102.1"
                     /translation="APHNQEAYKEYSFLSLPLTERIHREVLSLPMDPTMSIDDINMVI
                     EAINRWKS"
BASE COUNT          519 a          273 c          206 g          414 t
ORIGIN      
        1 ttatagggcc ttttttgtca gatgccaaca ataaataata cacaccacta taaaatacaa
       61 tgcataagtg atgctgtagg caatactaac ggcatttagt ccaaaattaa ttatcaaaaa
      121 ctttaccaat actggaaaaa ttattgaaaa catcacctcc aatatagcat ttattttaaa
      181 atatcccttt gctaacaata tagtggcaaa cagccaacta caaatccgca gaaaatcccc
      241 gatattttga aataaaaaaa gctcccgagc acgtataaag tcagccgtga acaagatact
      301 aattataaaa tctttcaagc cgtagatggt taaagccatg ctcaacgcga gcggtacaac
      361 aatacaagcc ccggtcttta aaagagttat atattcgcta tatcttcttg cggctgcagt
      421 ttttggaaaa taataaactg tcaatgccgt agtaagaaca gccagataag cctcagaaat
      481 tctatttaca gcttgccaat atccggcatc ctctaaagaa aaattattag tgagaatagt
      541 tcgaacaact atcatcgaaa taggtccagt taacgctcca atcaccccca tataaaagta
      601 atttttcatc tgagtaatgt tatgccgagg agtatgtccc acccagtatt taaatttaaa
      661 ccatggtctt ttaattataa tagtaataag ccagacacct gcaaccgcat tatttaatga
      721 tgcagacact aatgcgcctt tgaggccaaa aaaataaact aagcccacca tagataacaa
      781 cgatgaggat atagctaaaa cattcgcaag gaaaaatcta ttatattcat ttagtccatt
      841 taaaacccct aaaaatatat tgtttactat attaagagga agagcgcata gagcaattat
      901 aattaaccaa tacaaatcac tttgataaaa taagtattct gatattttac tggaaaaaag
      961 cacccccgta cttataataa aacaacaggc tatgaaggat aatttaattg atgcacgcca
     1021 ataaaaacat gcattttcgt aatcaccttg attttctgct gaatatctat taactccttg
     1081 tgaaacttgt gatgaaatga atccatttat aaaggccaca aaattttgca attgtcccaa
     1141 atttgctatt ccaattggac cagtataaat tgcaacaact tttgaaataa gaaggccaca
     1201 aattagtcta aagcatgtta ctacaccact gagtatcgta acttttgcga aatttagctt
     1261 ttccatctat taatcgcttc aattaccata ttaatatcgt cgatgctcat tgtcggatcc
     1321 attggcagcg ataagacttc tctatgtatt ctctcagtta aagggagaga taaaaaacta
     1381 tattctttat atgcttcttg attatgaggc gc
//
LOCUS       DAZGCS010000335         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGCS010000335 DAZGCS010000000
VERSION     DAZGCS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.342"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..544
                     /locus_tag="ACHK01_005376"
     CDS             <1..544
                     /locus_tag="ACHK01_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO8100103.1"
                     /translation="QAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLR
                     RFHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSV
                     LVDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGV
                     LKPAVLERQRKSKGVPRGEA"
     gene            531..>1369
                     /gene="repC"
                     /locus_tag="ACHK01_005377"
     CDS             531..>1369
                     /gene="repC"
                     /locus_tag="ACHK01_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFO8100104.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRP"
BASE COUNT          249 a          425 c          487 g          208 t
ORIGIN      
        1 gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat
       61 ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct
      121 ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt
      181 catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca
      241 tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag
      301 ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga
      361 ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag
      421 caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt
      481 gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc
      541 ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg
      601 gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg
      661 actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc
      721 tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg
      781 aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg
      841 tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg
      901 cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga
      961 aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt
     1021 acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg
     1081 ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca
     1141 gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg
     1201 gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga
     1261 ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct
     1321 ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaa
//
LOCUS       DAZGCS010000336         1358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGCS010000336 DAZGCS010000000
VERSION     DAZGCS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.343"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHK01_005378"
     CDS             <1..375
                     /locus_tag="ACHK01_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8100105.1"
                     /translation="LFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGFYRVPL
                     SSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRH
                     RHWHASAFTGICNDLAAASACM"
     gene            372..953
                     /locus_tag="ACHK01_005379"
     CDS             372..953
                     /locus_tag="ACHK01_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8100106.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQR"
BASE COUNT          323 a          327 c          423 g          285 t
ORIGIN      
        1 ctgttccggg atgcatacca tctggcggga attgatatgc cggattttga gcgtgaggat
       61 gactggtggc gcaacggtca gaacctttac ctggacaata tggcggtcac aggcttttac
      121 cgggtgcccc tgtcctctgc acaggcgggc gatatcctgc tgtgctgctt tggcgcatcg
      181 gtggccaatc atgccgccat ttactgcggc aacggtgaac tgcttcacca tctgcctgaa
      241 caactgagta aacgggagag gtattccgaa aaatggcaac gacgaacgca ttccgtctgg
      301 cgtcaccgcc actggcacgc atctgccttc acggggattt gcaacgattt ggccgccgcc
      361 tcagcctgta tgtgaacacg gcagcggaag ccatccgtgc cctgtcgatg cagatgccgg
      421 gattccgccg ccagatgaac gaaggctggt accagatacg tattgctggt gaggacacgg
      481 caccggaggc ggtgtatgcc cgccttcacg aacaactggg tgagggggcg gtcatccata
      541 ttgtgccgcg actggccggt gcagggaaag gcggactgca gattgtgctg ggggcggcag
      601 ccatcgtggg ctctttcttc acggccggag catcgatggc agcctggggt gcagcgctga
      661 gtgccggcgg tttttctgcc accacgatgc tgttttcact gggtgccagc atgatactgg
      721 gtggtgtggc acagatgctg gccccgaagc caaaaacacc ggaatacagg gcaacggata
      781 acggtaaaca gaacacgtac ttttcgtcgc tggataacat gattgcccag gggaacccga
      841 tgccggtgcc ttacggtgaa atgctggttg gctcccggcg aatctcccag gacatcagca
      901 cccgtgatga aggcgggggc ggaaaggtcg tggttatcgg gcggcagagg taaaaagaat
      961 aaaaaaatcc cgcagagttg cggggacaga caaagattaa cgttaaggag ttatttttgt
     1021 ttctggtgct cgggcaaaaa aaacattaac gcagagaaat tattagcacc acagtcagtt
     1081 tatgaaaatg tgaagatatt cagaagtttt attcagtcat gatacaggca tcctccggga
     1141 tgcctgttgt ttttgtgcgt aacagttatc acagtaaagg gtgagacaat gggcaaaggt
     1201 ggcggcaagg cgcacacgcc ggtagaggca aaggacaatc ttaagtccac gcagatgatg
     1261 agcgtgattg acgccattgg tgaagggccg attgaaggtc cggtgaaggg gttgcagagt
     1321 atcctggtga acaaaacccc gctgacggac acggacgg
//
LOCUS       DAZGCS010000337         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGCS010000337 DAZGCS010000000
VERSION     DAZGCS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.344"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..1168
                     /locus_tag="ACHK01_005380"
     CDS             23..1168
                     /locus_tag="ACHK01_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO8100107.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPFSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          458 a          276 c          243 g          364 t
ORIGIN      
        1 aaatgagata ggggaatctc tcatgataaa cggacttaat aataattccg catctttagt
       61 tttagatgct gcaattagaa ttaattctga ttttaaaaag cagtggaatg atatgtcatg
      121 cgctgaaaaa ttactaaaag ttcttagttt tggtttatgg aatccaacgt atactcgtag
      181 tgaaaggcaa acatttcaag agttgttaac cgttttagaa cctgtatctc ccgcgcctaa
      241 tgaattgggc agaatatatg ctaattttgc agatggctca tccttaagaa tttccgtcac
      301 taacagcgaa cttgttgaag ccgagattcg cacaccagat aatgaaaaga ttcttatgct
      361 cctggagtca aacgaacaaa ataggttatt acaatcttta cccatcaatc tccacatgcc
      421 atacattcag gttcatcgtg ccttatctaa gatggacctg actgatcata agtcaatgca
      481 caatctactt agttttacgt caaaactatc agcaaccttg attcctcata atactcaaac
      541 agatccgttt tccgggccta caccattcag ctctatgttt atggatacat ttcgaggact
      601 cggcaatgca aagctttcac tcaatggtgt tgatatacct gtagatgcac aaaaattgct
      661 tcgcgatgca ctaggactta aagacacaca ttcatcactt gcccggaatg ttataaataa
      721 tggtatttct cgtcatcatg cagagcaaat agcaagggaa agcagcggca gcgataaaca
      781 gaaagctgaa gttgtggagt ttttatgcca ccccgaagcg gcaacagcca tatgctcggc
      841 tttctatcaa tctttcaatg tgccagcctt aatgttgaca catacaagga tctctcaagc
      901 cagagaatac aatgtggaaa gatcgttaga tgtacctaac gcgtgcatta acataagtat
      961 ctctcaatca ccagatggaa gcattcacgt tgccagccat accgggattc tgataatggc
     1021 gccagaagac cgccccaacg agttaggaat gttgacgaac aggacctcct atgaagtgcc
     1081 acaaggtgtg aaatgtgaaa tcgatgagat ggtaagaacg ctacaaccaa ggtatggcgc
     1141 atctgaaaca tacctgaaaa acatttaaaa ataaaaaaat ggacaccaca cactttattt
     1201 atcaaaacaa acaataacta atcacactca caattattaa taatttaacc tcacagcaat
     1261 catacaaaac aaagatattt atttcgcaaa ccatttatag caaatggaga aggaatgaaa
     1321 agaagcttat ttattgcatt t
//
LOCUS       DAZGCS010000338         1333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGCS010000338 DAZGCS010000000
VERSION     DAZGCS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.345"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(221..796)
                     /locus_tag="ACHK01_005381"
     CDS             complement(221..796)
                     /locus_tag="ACHK01_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO8100108.1"
                     /translation="MPLTSGIGSHSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TDGSFSSSGGNGGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQEGIQSKISQYK
                     FSVCPERLQCPPESAQCPITLEIPEEGVFIKNSGDSVVCSLFDVTAFSRLVSEKSPHP
                     LTREKLTASMVVSADKCFYDHGKGSFVIKDS"
     gene            953..1207
                     /locus_tag="ACHK01_005382"
                     /pseudo
     CDS             953..1207
                     /locus_tag="ACHK01_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          419 a          314 c          209 g          391 t
ORIGIN      
        1 atacacacac ctaatctcaa aaaaacaaac ttactatttg acttggattc tgtttttaac
       61 ccaatatttt ccgacagcat ctccacagaa aaatgcattt acgtaaagac gccctcaagt
      121 catctgaaac ctgtcaacca caggcatact cttgtattct tgatgcctgt ggcgttttta
      181 caaattactg acagctaata ttcgctcaga gaaaaatatt ttaactatct tttataacga
      241 agctaccctt accatggtca taaaaacatt tatcagcact tacaaccatt gatgccgtta
      301 gtttctctcg tgtcagtggg tgaggtgatt tttcactaac taaccgagaa aatgcagtaa
      361 catcaaataa actacataca actgaatccc ctgaattctt aataaacacc ccttcctctg
      421 gtatctccaa tgtaatggga cattgagcag actctggcgg gcactgaagt ctctctggac
      481 aaacagaaaa cttatattga gatattttgc tctgaatacc ctcttgcgat tgtgcttcac
      541 ctgagtccga aagcatactt agcattctct cactaagggc tcgaggacca ttattaaatc
      601 ccaaaatcaa taactcagaa agcaatccgc cattaccgcc actggatgaa aagctcccat
      661 cagtagcatc atacacaata ctgacagttt caccacctac tgtaatatct ccgcgcccat
      721 tggctgcaat tcgggcacga aaaacctcca gcccaagatt aaatgaatgt gatccaatac
      781 ctgaggttaa tggcataaca aaactcccta ttttaatttg aactccagac ttaaatagct
      841 gtaacaaaca tctgccttat atggcaacac aaaaaccgga gccggactcc gttttttgtg
      901 tgtcgggctg ttcattctat cctttcaaca ttccgtctga ttggcatttg taggtccagc
      961 aacagtcagt cacgatgtta gttatcttgg cagtgttctg gatgcagcca aacctatata
     1021 cggaattaat tacacatcaa acccggcgaa aagtgctcgt ccatatctac ttaaacttgc
     1081 tttgattggt aaatcaaacc gtcgtaatcg tagaccagca gttgatgaac ttgacatgct
     1141 cattgaagcc cttcaacaac gatctactca taaatgttca aaaattccgt tcgttgatat
     1201 cctcaaaagt tcagcctagg ctatgctgaa ccggttttcc tgaatcattt taccgtatgg
     1261 ttaaatttta acgtcataat acttattatt atgtttacaa ataattgcaa ttttaacaaa
     1321 agctaactac ata
//
LOCUS       DAZGCS010000339         1333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGCS010000339 DAZGCS010000000
VERSION     DAZGCS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.346"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..269
                     /gene="nleC"
                     /locus_tag="ACHK01_005383"
                     /pseudo
     CDS             3..269
                     /gene="nleC"
                     /locus_tag="ACHK01_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            316..426
                     /locus_tag="ACHK01_005384"
                     /pseudo
     CDS             316..426
                     /locus_tag="ACHK01_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            590..1333
                     /locus_tag="ACHK01_005385"
                     /pseudo
     CDS             590..1333
                     /locus_tag="ACHK01_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
BASE COUNT          391 a          233 c          316 g          393 t
ORIGIN      
        1 atataaacat gaaaattccc tcattacagt ccaacttcaa cttttccgcc ccggcaggat
       61 actctgctcc cattgctcct aatcgtgctg aaaatgccta tgcggattac gttttggata
      121 taggtaagcg aataccactt tccgcagcag atttaagcaa cgtatacgaa agtgtaatac
      181 gcgccgtcca tgacagtcgt ggcagactca ttgatcagca tacggtcgat atgattagta
      241 atactatact tgatgctttg agccgctcat aaacttttcg tgatgccgta agctatggca
      301 ttcatattca ggatgctgca gatcgcaacg gatgaagaga catcgttgtt gacagtctgg
      361 aagaggtatc gggtgttact gaaccgtgtt gatacgtcag tatctccaga tttcgagggc
      421 cagtaatacc tgatttatag ttcgtaaacg ttcgtctgac gggatgctgg aaggatgaat
      481 gagttgccag atactcaaaa acaatagtta atttccagtt ttttgttgtc atgtcatggt
      541 gatgtttgtt aagaaagtaa agattgattc attttgaagg ttgaaatgta tgctatcacc
      601 atcttctgta aatttggggt gttcatggaa ttctttaacc agaaacctga ctttgcctga
      661 taatcgtgtt ttatccctct gtaagggatg ctgccgctca ctctgataat ggggcgcaag
      721 taaaggttag caacagaaca tatcgcgttg ttttaagtga taataggttt tgcgtgacaa
      781 gagagagtca tagtggatgt tttactaata tgctgtatag actgggatgg cctaagggag
      841 agatcaccag aaaaattgag gctatgctga attcatcgac agtgagcacg actatagaaa
      901 gaggatctgt tcgttcgaac agacctgatt tacctccagt ggattatgcg cagccggagt
      961 taccgccagc ggattatact cagtcagcgt tgctgaggct tagcaacaac aaatcacccg
     1021 tgctaggtaa cgttattggt aaaggtggta atgctgtcgt gtatgaagat atggaaaata
     1081 caacaaaagt gttgaagatg tttactatat ctcaaagcca tgaagaggtg acaagcgaag
     1141 ttcgttgttt caaccagtat tatggtgccg ggagtgcaga gaaaatatat ggcgataata
     1201 gagatattat tggtattaga atggataaaa taaatggaga atcgctttta aatatttcgt
     1261 ccttgccagc acaggctgag catgctattt atgatatgtt tgagagactg gaaaaaaagg
     1321 aattcttttt gtt
//
LOCUS       DAZGCS010000340         1330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGCS010000340 DAZGCS010000000
VERSION     DAZGCS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.347"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(102..259)
                     /locus_tag="ACHK01_005386"
                     /pseudo
     CDS             complement(102..259)
                     /locus_tag="ACHK01_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            588..1239
                     /gene="nleG7"
                     /locus_tag="ACHK01_005387"
                     /pseudo
     CDS             588..1239
                     /gene="nleG7"
                     /locus_tag="ACHK01_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
BASE COUNT          441 a          285 c          204 g          400 t
ORIGIN      
        1 gtaaaatcag taacggctgg aaatcattca atactcacac tatctaaaag ttcaccagcc
       61 aacagcggta agttctttca tacgacgtgc cgcggatcct attattctag tatgaaatcc
      121 ctaaccgatt tcaaattctc cagactgctc agattctctt ttatcccacg ctggcgctga
      181 taaatctcgt cattgcaatc gacctgcgct atcgctgccg cagttcttcc agtttctgca
      241 tcgataactc catcttctga gcaataaacc tcacctatgc aatattttat gtcaattatt
      301 tcaattgcat taatatcttg aatggattac atagagttaa tgtatccagt actccctatt
      361 ctcttgcata taatacatgt tgcaacttac atctcagcgc tatgaaaaaa caccacctct
      421 ctctttatga aattctggat ttaccaagcg ctaatttatc atttcaatct acctttaagc
      481 attgcattta tctccctacg aggtcatact ttaggaagtt aaatatgaat gataatatac
      541 ctacagcgcg aaatcacaaa caatcgactt gtattacaga aaaaaacatg cctatctttt
      601 taaacttcac agcaggcagt atcttacctg agaatgagct agcatcttta cgttatattg
      661 tgcagcaaaa tcaaaatgat actataatca taaaagaacg ttataaaatg gatatccgtt
      721 atatcgaatc agtcaatggt tttacagtaa atcctgtatg cagtaatcat ttctccatat
      781 ttatggcgag acaaaacact attgctcgca acctggaaca gcagatcaac aacggacgaa
      841 gttttgcaca aatatctcag gattttatgc ttcaattatc ttcaaatata ggatggaaaa
      901 aaggggccga aaacgccctt aaaaataaaa tccattctca ttcatttgtt gtaaatcctg
      961 atgaattctc ttgcgacaca caatttctta agtgcccaat aacattatgc gttccagaaa
     1021 aaggggtttt tgtcaagaac gcactgaact ccaacatatg cactctttat gataagtctg
     1081 cattcatgaa tctcacaaga gaacatctac cccaccctct cagcagggag agggatagta
     1141 aaagaaatga ttattgaaag gaatatgtgt tattttgaca ccataagtca gcatttcata
     1201 attatggatg cagaccaaca gaaacagcat tgtaaataaa atgtaataat tacatactat
     1261 tagtgattct catgcatcgt aagcggctcg ccagaaccgt attgatattt actgagcgct
     1321 cagatcaact
//
LOCUS       DAZGCS010000341         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGCS010000341 DAZGCS010000000
VERSION     DAZGCS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.348"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            352..798
                     /locus_tag="ACHK01_005388"
     CDS             352..798
                     /locus_tag="ACHK01_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100109.1"
                     /translation="MDRDDILDRVLYGYFLEQLFSVATGRLDKLLSVVSIILGSSVIG
                     GFIPEVSGVLIVVIATVQTIYGFGQKSGNAMRKSAEYLQLYDDAEKYSDSELKMQLKL
                     LEKTDDNIWSSLKDIAILKTQIKIGISVEQQEKLSAKSKLMRFLCG"
     gene            complement(897..1048)
                     /locus_tag="ACHK01_005389"
                     /pseudo
     CDS             complement(897..1048)
                     /locus_tag="ACHK01_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
     gene            complement(1059..1238)
                     /locus_tag="ACHK01_005390"
     CDS             complement(1059..1238)
                     /locus_tag="ACHK01_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100110.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
BASE COUNT          359 a          307 c          292 g          355 t
ORIGIN      
        1 tgccacaacc ggaagcgcac gttcgaagaa atctaacgac aagccttcta agggaaagag
       61 cttcgccgta cgctttcgcg ttgtgtgcct gcttttaacc acgtcaggcg aggtggtttc
      121 cgtcgttccc caacgacagg aaatctgtat aatctggata tccccaacaa caggaaggtg
      181 ttttatatgg tggttcaaaa gaattgttca ggtactggta agcctgcgga caggtcggac
      241 attccgaata ccaggaaaca tatacctgag aaaaacattc gaccaaaacc atctccagct
      301 ccttcagagg agagggggaa cagcaacaat caaacaagca ggtgatgata tatggaccgg
      361 gatgatattc ttgacagggt tttatatggt tattttcttg aacaactatt ctctgtagcg
      421 actggtcgtc tcgataaact tctctcagtg gtgagtatta tcctcggttc atctgtcatt
      481 ggtggattta ttcctgaagt ttctggagtt ctcattgttg tgatagcaac cgttcaaacg
      541 atttacggat tcggacaaaa gtcaggtaac gcaatgagaa aatccgcaga atatctgcag
      601 ctttatgatg atgcagaaaa atattccgat tcggaattga aaatgcagtt aaagctcctg
      661 gaaaaaacag atgataatat ttggtcgtcg cttaaagata tcgcaatctt aaaaacccag
      721 atcaaaatag gaatctccgt agaacaacaa gagaaactgt ctgcaaaatc caaattgatg
      781 cgattccttt gtggttagga atacccagat tgttaaagag catgccggat gcttacccgt
      841 gtccggcgaa cgtattccac ttcgcctgtg gagaactctt taattactaa ccttcatcag
      901 tcagagtttc ttgctaacca gcgacgcgcg ccagcttccg ttttaaacgt tttgcttctg
      961 gtaacgtcat cgcggtgaac gttccatcct ggttggggaa cacgccgcac accagggatt
     1021 cgttgttgcc gaggtcgatt ttttgcattt tgcgaatctc acatcttgtt gctacgtata
     1081 gcgacttctg cctgccagag atcccagtcg ttgctgcgta aagcctgcac agcctggttg
     1141 taagtgatac cgcaacaatc catcaaatac tgaactactt cgtaatgcac catcttatct
     1201 atccccttaa cgccgggtgg cggaactaac tgctgcactg caaaatttga atcccgccgt
     1261 catgttcata cgcctcgggc tggctactta acccctgacc actgcctggt aac
//
LOCUS       DAZGCS010000342         1295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGCS010000342 DAZGCS010000000
VERSION     DAZGCS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.349"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..171)
                     /locus_tag="ACHK01_005391"
     CDS             complement(<1..171)
                     /locus_tag="ACHK01_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8100111.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQC"
     gene            complement(396..950)
                     /locus_tag="ACHK01_005392"
     CDS             complement(396..950)
                     /locus_tag="ACHK01_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO8100112.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(959..>1295)
                     /locus_tag="ACHK01_005393"
     CDS             complement(959..>1295)
                     /locus_tag="ACHK01_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8100113.1"
                     /translation="YPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSG
                     RLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQLVSGGRLG
                     VKIYKIESE"
BASE COUNT          311 a          364 c          257 g          363 t
ORIGIN      
        1 gcactgatag tctgccagac ccgccataac cagctgggtc agtattaact ggcagcgttc
       61 gcgtgaaagg taagtattct gcgcaatctc cccgactgtc gccggttcgg taacgcttaa
      121 ttcattaaac accactctgg cggtttctgt catatcctgc tgttttagca tgtctttttc
      181 ccttttccgg ttaacgtgac ataccaataa ctcttgtcga aaaagccagc aagttgaaag
      241 accggtatta gcaaccacca gcgcgtttaa cgccccgtgc cgtttttcag tcataaaaaa
      301 acccgcaaaa agcgggctct ttcaaatgtc catgtctgct attcgcctcg cggtacagct
      361 ttgcgaagct taccggaatt gaagcagttt ttacgtcaaa aagcaataac ttttttctct
      421 ataccaaaag ccataaccat tggtttgtac aaaataaatt ctgccacctt tagccaatgc
      481 tcaatgcgtc tttcacaggt tcttaaactc cattccggat gtgcatcatt cagcaattca
      541 gccattttgc gcttagtcat cccccgcccc acataacgct gactcagaac attgagcagc
      601 ccgggataac ctgccagtac ttcaccaata accctgtcga ttattaacgc ctctgaatcg
      661 gtacaatgtg ccagccagct tttttgattg ccgttgatca tatcccgcaa aaaagcctca
      721 agttcaggtt tgtccagacc cgcttttttc atcctccgga gcgcctcgtt aattgccgtt
      781 tttgtcagct ttttagaggt cagtaattgg ttgaacatat ttcccgtctt accgtcgcca
      841 atatacgacc aacgccccca catacgcagt ttcccctgga tccagacact ttccagcgtt
      901 ttcaggcgta aatgctcacc gcttttgcct gtaatttccg ggtatatcat atttatgctc
      961 actcactttc aattttgtaa atcttcacgc ccagccgccc accagaaacg agctggccgc
     1021 gcacaatatt gatttcatca aactgctcgt cgtctatgag aagtccggca tgcgtcagcg
     1081 catccagtgg tgctttcagg atattgtcca ggtcacgacg gcgcttatcc ggtggctctg
     1141 caataatctt tatcgccagc cttccggaca ggtttaattt cagccgctgc tggcgaacaa
     1201 taagtgccac atcccggcga taacgctcac cggcttttga tacaaaatat gtgctgccac
     1261 gacgtcgcca gtaggtgttc accgtcggcg ggtaa
//
LOCUS       DAZGCS010000343         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGCS010000343 DAZGCS010000000
VERSION     DAZGCS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.350"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..160
                     /gene="azuC"
                     /locus_tag="ACHK01_005394"
     CDS             74..160
                     /gene="azuC"
                     /locus_tag="ACHK01_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO8100114.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            274..525
                     /gene="yecJ"
                     /locus_tag="ACHK01_005395"
     CDS             274..525
                     /gene="yecJ"
                     /locus_tag="ACHK01_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO8100115.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(604..1107)
                     /gene="ftnB"
                     /locus_tag="ACHK01_005396"
     CDS             complement(604..1107)
                     /gene="ftnB"
                     /locus_tag="ACHK01_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO8100116.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          362 a          285 c          297 g          347 t
ORIGIN      
        1 atttttgtga tggttgtcat attataagcg caaagagaat aacacctgta taacaaatgg
       61 tcggagtgcc gcgatgaaac tgcgcaaaat cctgaaaagt atgttcaata actattgcaa
      121 gacgttcaaa gacgtaccgc caggcaatat gttccgataa taaaaaacct gctccggcag
      181 gttttttgtg tcctgatgac ggtggatgtg actatcgctg aagatgtgaa gccgctacta
      241 taacggcttt caaaatgcta tcaggagttt acgatgtccc agccgctgaa tgccgatcag
      301 gaactggttt ctgatgtggt tgcttgccag ttggttatca aacaaattct ggatgttctc
      361 gatgtaatcg cgcccgttga agttcgtgaa aaaatgtcca gtcagctgaa gaatatcgac
      421 tttactaacc atcccgccgc cgcagacccg gtcaccatgc gggcaatcca aaaagccatc
      481 gcgcttattg aactcaagtt taccccacag ggtgaatccc attaaaaaaa ccgctgcttc
      541 aaacagatga ggcagcggtt tctcaaaacc ttcatcggcg caatgcatta gcgccgatga
      601 tgatcaatgc agctggtgtg acacgacatt cagaacatgt tggtcggtct gcacagggca
      661 catacccgca agtttcgcac tgcgcacttc atcaagaatg gtttgcagca acagaccatc
      721 atgctgctgt tctttttcca gatcgcgcag gaaattgacg gttgaatcat cattcagttc
      781 tttcgcttca tcggctaact gtgccaacgt actagaacgt tgctcgtatt cttccatcgt
      841 tttttggaac agttcttcca gagagttcag tttttcaccg ggaacatcaa tggctttaac
      901 gatgggggta gcgccgacac tcttcataaa gttaaacatg cgcatcattt gggtcacatt
      961 actctgtgcc tgggcgcgaa ggaaagtggc ggtgccgttc agactctgtt cagaacacca
     1021 gttactcagg tgaaggtaga gattggatgc gtaaaactcg cggttcattt gagagttgag
     1081 tttgagaagc attccagcgg ttgccatacc taatatcctt atatccagaa gtggagaggt
     1141 gcaagataaa tcaaaacacc ataattactg tttgcagaat aattcctttc tgccggtccg
     1201 aaatgtgatc gtggagtcaa ttctgacgat gatctgaaat aagaataacg tctgaacggg
     1261 tataagaaaa tgcttaaatc atcaagttta t
//
LOCUS       DAZGCS010000344         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGCS010000344 DAZGCS010000000
VERSION     DAZGCS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.351"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..927
                     /gene="nleB2"
                     /locus_tag="ACHK01_005397"
     CDS             <1..927
                     /gene="nleB2"
                     /locus_tag="ACHK01_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO8100117.1"
                     /translation="SPCQTVSFAGKEYELKVIDEKTPILFQWFEPNPERYKKDEVPIV
                     NTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFSKLERDFENVMIIYREDVD
                     FSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELREISKAQDSLISMYAKKRN
                     HAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLDMDMILTGKLGTIYAPDGI
                     SMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVDAHPYYDGLGKGVKKYFNF
                     TPLHNYNHFCDFIEFKHPNMIMNTSQYTCSSW"
     gene            961..1248
                     /gene="nleC"
                     /locus_tag="ACHK01_005398"
                     /pseudo
     CDS             961..1248
                     /gene="nleC"
                     /locus_tag="ACHK01_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
BASE COUNT          413 a          198 c          258 g          408 t
ORIGIN      
        1 tcaccctgtc aaacggtttc ttttgccgga aaggaatatg agttaaaggt cattgatgaa
       61 aaaacgccta ttctttttca gtggtttgaa cctaatcctg aacgatataa gaaagatgag
      121 gttccaatag ttaatactaa gcagcatccc tatttagata atgtcacaaa tgcggcaagg
      181 atagagagtg atcgtatgat aggtattttt gttgatggcg atttttcagt caaccaaaag
      241 actgcttttt caaaattgga acgagatttt gaaaatgtaa tgataatcta tcgggaagat
      301 gttgacttca gtatgtatga cagaaaacta tcagatattt atcatgatat tatatgtgaa
      361 caaaggttac gaactgaaga caaaagagat gaatacttgt tgaatctgtt agagaaagag
      421 ctgagggaaa tttcaaaggc gcaggattct ttgatttcta tgtatgcaaa gaaaagaaat
      481 catgcatggt ttgatttctt cagaaattta gccttattaa aagcaggaga gatattcagg
      541 tgcacatata atacaaagaa tcacggtatt tcattcgggg aggggtgtat ctatcttgat
      601 atggatatga tacttacagg taagcttggt acaatatatg ctcctgatgg aatttcaatg
      661 catgtggatc gtcgtaatga tagtgtaaat attgaaaata gtgcaataat tgttaaccgt
      721 agtaatcatc ctgctctact tgagggactt tcttttatgc atagtaaagt agatgctcat
      781 ccatattatg atggtttggg gaaaggagtt aagaaatatt ttaactttac accattacat
      841 aattataatc atttttgtga ctttattgag tttaaacacc ctaatatgat catgaacaca
      901 agtcagtata catgcagttc atggtaaatg aatttgataa agtttattct gttgtaataa
      961 atgatttgca tggtattagg tatcaacatg aaaattccct cattacagtc caacttcaac
     1021 ttttccgccc cggcaggata ctctgctctc attgctccta atcgtgctga aaatgcctat
     1081 gcggattacg ttttggatat aggcaagcga ataccacttt ccgcagcaga tttaagcaac
     1141 gtatacgaaa gtgtaattcg tgccgtccat gacagtcgtg gcagactcat tgatcagcat
     1201 acggtcgata tgattaataa tactatactt gatgctttga gccgctcata aacttttcgt
     1261 gatgccgtaa gctatgg
//
LOCUS       DAZGCS010000345         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGCS010000345 DAZGCS010000000
VERSION     DAZGCS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.352"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..630
                     /locus_tag="ACHK01_005399"
     CDS             <1..630
                     /locus_tag="ACHK01_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8100118.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASS
                     APNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPT
                     TQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            627..1196
                     /gene="ybfC"
                     /locus_tag="ACHK01_005400"
     CDS             627..1196
                     /gene="ybfC"
                     /locus_tag="ACHK01_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO8100119.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIEIQHELIRSEEK"
BASE COUNT          420 a          235 c          278 g          325 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      121 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      181 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      241 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      301 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      361 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
      421 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
      481 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
      541 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
      601 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
      661 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
      721 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
      781 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
      841 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
      901 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
      961 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1021 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1081 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1141 cttacgaatg tttaatagag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1201 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtg
//
LOCUS       DAZGCS010000346         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGCS010000346 DAZGCS010000000
VERSION     DAZGCS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.353"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHK01_005401"
     CDS             <1..375
                     /locus_tag="ACHK01_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8100120.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHK01_005402"
     CDS             650..1219
                     /locus_tag="ACHK01_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO8100121.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
BASE COUNT          354 a          257 c          347 g          297 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtggac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cataa
//
LOCUS       DAZGCS010000347         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGCS010000347 DAZGCS010000000
VERSION     DAZGCS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.354"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..>1252
                     /gene="nleA"
                     /locus_tag="ACHK01_005403"
     CDS             36..>1252
                     /gene="nleA"
                     /locus_tag="ACHK01_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO8100122.1"
                     /translation="MNVQPNVHTGLTPPLHLAHPPHPAPNQIPESELPPGCETGFVVN
                     IPEDIQQHTPECGETTALLGLIRDKGLLSGLDAYLAPHLEEGSAGKKALDMFGLFNVT
                     QMALEIPSTVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPLQ
                     KFLSQNPNPWFGVTAQVVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVIR
                     PGEFDLKYGSTPLQSTLGLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQY
                     TGTDAMLSPHVGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAETGEGRF
                     TDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGL
                     PHHNPSNHVDIFD"
BASE COUNT          381 a          269 c          278 g          324 t
ORIGIN      
        1 attaatggat ataaactatg taataaggat ttattatgaa tgttcaacca aatgtgcaca
       61 ctggattaac ccccccctta catcttgcac acccaccaca tcctgcgcca aatcagatac
      121 cggaatcaga attgccacct ggatgtgaga ctggatttgt tgttaatatt ccagaggata
      181 tacagcaaca cactccggaa tgcggtgaaa caacagctct gttgggttta ataagagata
      241 aaggtctgct ctcagggtta gacgcatatc ttgctcctca ccttgaagaa ggctcagcag
      301 gaaaaaaagc attggatatg tttggtttat tcaatgttac tcagatggca ttagaaatac
      361 ccagcaccgt tcctggtatc tctggtaaat atggtgttca gatgaacatt gtaaaaccag
      421 atattcaccc cacaaccggg aattattttt tacagttatt ccccctacat gatgaggtgg
      481 gaataaactt cggtgcgctc ccaggaccac tccagaagtt tctctctcaa aatcccaatc
      541 catggtttgg agtaacagct caagttgttc gtaaccacgg catagaacta cctatagtca
      601 aaacagataa tggatggaaa ctagttggag aaacaccact tacacctgaa gggcctaaag
      661 caaattatac ggaagaatgg gtcatcagac ctggtgagtt tgacttaaag tatggcagca
      721 ctccgttgca gagcacttta ggtttagaat ttggagctca ttttaaatgg gatttagata
      781 atcctaatac caaatatgcc gttcttacca atgcacttgc gaatgcagct ggtgctgcag
      841 ggggatatac tctatctcaa tatacaggaa cagacgcaat gctgagcccc catgttggct
      901 cgatggccgg acaggctttg gggcatgctg tacaatgcta cactccagga ttaaagccag
      961 acactatttt atggtgggca ggtacgactc tggggctggc agatttaaac aaagccgaga
     1021 caggtgaagg cagattcact gactatcctc gtatatggtg gcacgcaaga gaaggtgcca
     1081 tcttccctaa taaagaagat attgcccgtg caacaggggc tgatatacga gctatggaag
     1141 aaggtatccc tgttggacag cggcatccaa atccagagga tgtggtaatc gatatcgaaa
     1201 gcaatggctt accacatcat aatccatcaa atcatgttga tatctttgat at
//
LOCUS       DAZGCS010000348         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGCS010000348 DAZGCS010000000
VERSION     DAZGCS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.355"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..306)
                     /locus_tag="ACHK01_005404"
                     /pseudo
     CDS             complement(1..306)
                     /locus_tag="ACHK01_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
     gene            695..1210
                     /gene="ehxC"
                     /locus_tag="ACHK01_005405"
                     /gene_synonym="hlyC"
     CDS             695..1210
                     /gene="ehxC"
                     /locus_tag="ACHK01_005405"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO8100123.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
BASE COUNT          367 a          208 c          255 g          418 t
ORIGIN      
        1 tgagcggtca gtggttctgg ctgacaaggg tgtgatttcg ccgttaggac caaacaccag
       61 tgagccggtc agcgtcatca caaactcttt cagctctttg ttgccgtcaa acattctgtt
      121 tttggaaagc gcattccaca tgatgttgat gtttttggtc acccgctcct tatccttgtc
      181 gctggcttta tccctgacac tgtcagattt cccgccaacg gtacatccct gccgggaagc
      241 cgcccagtca gcaaaaatat tgctctcacc ggcaatgtcc tgacagactt tctgctggga
      301 cacctgcggt tcaaggtttg cttttctgtg taggtttaga ttttgtgttt gcagggtaaa
      361 caaaactgat taactcgtca tttcggtaac tgaaatagtt tgctgtattc tgtttacaat
      421 ctctttaata aggcatttgt cacgtggcta ttcatatgaa aatcatacgt tatcttacaa
      481 atcaatcatc tgagtgttat aatataactt agctgtgata tgtgtaagaa tgtttaggca
      541 atatattatt atcgctaatg ttgttttggt tgggagagat tctccttgcg gtagcttttt
      601 gtttttagat gcttcttgct taaaaagata taatttctgt tcttttatat aggattcttt
      661 acatttactg agttttctta tttatttaaa cattatgaaa tctaatgctt ttgacgtcat
      721 ggggaaggtt gcatggctat gggcctgttc tcctctgcat aaaaaatggc cattatctgt
      781 ttttgctatt aatgtcatac ctgctattca gacaaatcaa tttgctctct taataaagga
      841 tgagcttcct gtagcttttt gtagctgggc cagtttagat ctggaatgtg aggtaaaata
      901 tataaatgat gtaacatctt tgtatgcaaa agactggatg tcgggagaaa gaaagtggtt
      961 tattgactgg atagctccat ttggacacaa catggagtta tataaataca tgaggaaaaa
     1021 gtatccttat gagttattca gagcaataag gttagatgag agcagtaaaa caggaaaaat
     1081 agctgaattt catggagggg ggatagataa aaaactggca tcaaaaatat tcaggcaata
     1141 ccatcatgaa cttatgtctg aagtaaaaaa cagacaagat tttaatttta atattgagaa
     1201 agaaaactaa tatgacagta aataaaataa agaacatttt caataatg
//
LOCUS       DAZGCS010000349         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGCS010000349 DAZGCS010000000
VERSION     DAZGCS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.356"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..429
                     /locus_tag="ACHK01_005406"
     CDS             <1..429
                     /locus_tag="ACHK01_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8100124.1"
                     /translation="QSLGKVRVPHRGNVVEKVIEGAYEVVGVFDRIEEKRDAMQSLVL
                     PPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGI
                     SGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            511..990
                     /locus_tag="ACHK01_005407"
     CDS             511..990
                     /locus_tag="ACHK01_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8100125.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPLWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1006..>1235
                     /locus_tag="ACHK01_005408"
     CDS             1006..>1235
                     /locus_tag="ACHK01_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO8100126.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETL"
BASE COUNT          296 a          331 c          342 g          266 t
ORIGIN      
        1 cagtctctgg ggaaagtgcg tgttccacac cggggaaatg tagtggagaa agttatcgaa
       61 ggggcttacg aggtggtggg cgtgtttgac cggatagagg agaagcgtga tgccatgcag
      121 tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
      181 tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccgcg acgccgggag
      241 gattacggta aggacctgtg gagcgcatat cagaccatcc aggagaatat gctgaaaggc
      301 gggatttccg gtcgcagtgc aaaaggaaaa cgtatccata cccgggccat tcacagcatt
      361 gataccgaca ttaagctcaa tcgcgcattg tgggtgatgg cagaaacgat gctggagagc
      421 ctgcgctgat gccgtttccc tgagtgaggg aatcattcag ctttcgttcc tgaaaaacac
      481 acatttattt gttatttaag gagttatctc atgaaaaccg tttctcagaa tacccccaca
      541 atttattcag ctacaacacc agagaataat ccgcctcagt tggttgccag cctcgtccct
      601 gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccact gtgggtgacc
      661 ctggagcccc gtgtcttcgg ctggatggac cgtctgtgcg aagactactg cggtggtatc
      721 tggaatctgt acaccctgaa caacggcggg gcatttatgg cacccgaacc ggatgacgat
      781 gatgacgaaa catgggtact gttcaatgcc atgaacggta accgcgcaga aatgagcccg
      841 gaagccgccg gtattgccgc ctgtctgatg acgtacagtc atcatgcctg tcgtacggag
      901 aattatgcca tgacggtcca ttattaccgg ttgcgggatt acgccctgca gcatccggaa
      961 tgcagcgcca ttatgcgcat catcgactga atggagagaa gcacaatgca acaactttcc
     1021 tttctgcccg gagagatgac gcccggcgag cgcagcctca ttcaacgggc cctgaaaacc
     1081 ctggaccgcc atcttcatga acccggcgtg gccttcacct ccacccgtgc agcacgggaa
     1141 tggctgattc tgaacatggc gggactggag cgtgaagaat tccgggtgct gtatctgaat
     1201 aaccagaatc aactgattgc cggtgaaacc ctctt
//
LOCUS       DAZGCS010000350         1228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGCS010000350 DAZGCS010000000
VERSION     DAZGCS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.357"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1228
                     /locus_tag="ACHK01_005409"
     rRNA            <1..>1228
                     /locus_tag="ACHK01_005409"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          309 a          285 c          386 g          248 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga
      721 gatggattgg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacct
//
LOCUS       DAZGCS010000351         1218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGCS010000351 DAZGCS010000000
VERSION     DAZGCS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.358"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            579..>1218
                     /locus_tag="ACHK01_005410"
     CDS             579..>1218
                     /locus_tag="ACHK01_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8100127.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAI"
BASE COUNT          394 a          238 c          218 g          368 t
ORIGIN      
        1 cgtaagaggc taacctagat ttgccgttca tagattgagc ctcagattaa tgttaagcgt
       61 cttgcaggac gcgtaatgtt aactggggct tttctctatc tgccgttggt gttcatgccc
      121 gaggcagata gcctcaagca cccgcagcta ttctacttaa cttcccatta cctcgccaat
      181 atgaaatcgg tcagaaagac agacgtccca taaggacaac aacggaaaaa gagattttgc
      241 ttgatgctat ttacctctgc accatgaagt tgcgccaaaa tcccagtggt gataacagga
      301 atttggttgt tggtgattgg ggaaagaagt acgttagact gtgggttagt tcattgacat
      361 gatagttacc tgccttcaat ttcctaagca agcgatgtaa acgataatca atatcaaatg
      421 tattgacagg taaaataata acgatgtacc tttgctgata acatatatca gtcttaaatc
      481 ataatggagg tagataaaat aactaaacac acacaacaaa ttttttatca gtgacatagc
      541 atatcttaag catcctttaa tgctcatggg gagaacgcat gactaaacat atggacattc
      601 attgtcaaac acgcatattt aactcaatta accatgacac caaatataaa atatgtattt
      661 ttactccaaa aaacattaaa aaaaataatg caactcttta tatacttgat gggaatagtg
      721 ccaacaatta catttctgat attctgcctg ttattgatgc actacccaat ccaccagtgt
      781 tagttacact tggttatgaa tcttggaata accttagcat tcatcgccgt gcttacgatt
      841 atactcctga tggtgaaaat gctattgttg ataactctaa accagcatgg atctacttta
      901 ctggtggagg aagccagtct ttccgagaat tattactaac tcagatcatg ccatgggtta
      961 gtactattgc tccgaacagt tccagaatcg gtatatgggg gcactcgcta ggtgctattt
     1021 ttgtgcttga ttgcttaaaa aataattcat gctttaacta ttattatata tctgcaccat
     1081 cacttctatg gcagaatgaa agaatcatca agataattaa agatgatata tcagaatcaa
     1141 agcacactaa aagtatatgc ttgcttaacg gaaatcttag ccttgattac tccgcatctt
     1201 tatatcctga agccatca
//
LOCUS       DAZGCS010000352         1218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGCS010000352 DAZGCS010000000
VERSION     DAZGCS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.359"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..815
                     /locus_tag="ACHK01_005411"
     CDS             12..815
                     /locus_tag="ACHK01_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO8100128.1"
                     /translation="MVVYRLIGVEESVAEPEYTQKRVSLKVGKIGAVNKNSTENGYVS
                     AQKSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEPKDLNPTHNARESAPISEQEVL
                     SLQAAPHVFLDGLSEPIGKFPMTDSWYPSRDFRRRAALWGMALPETEFTPAELTAFRD
                     YWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVNHAAAPGGTASRAVQEIRAARE
                     QWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRTFEALDCTDWRDD"
     gene            808..>1218
                     /locus_tag="ACHK01_005412"
     CDS             808..>1218
                     /locus_tag="ACHK01_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8100129.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYNGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPLGPTPI"
BASE COUNT          336 a          293 c          357 g          232 t
ORIGIN      
        1 gaacaaagca aattgtggtg taccgactta tcggtgtaga agaaagtgtt gccgagcctg
       61 aatacaccca aaaacgggtg tctttaaagg tgggtaaaat tggtgctgtt aataaaaaca
      121 gtaccgaaaa cggttatgtt tcagcacaaa agagccccaa aaacggaact ctttgttgca
      181 tggaaaataa ccaaagacac ccaaattttc catcaaagac acccaaaaac ggatcacgga
      241 acccaaagga acccaaagat ctaaacccca cacataacgc acgcgagagt gctccgatca
      301 gtgagcagga agttttgtcg ttacaggcag ccccccatgt attcctggat ggcctgagcg
      361 aacccatcgg aaaatttccg atgaccgata gctggtatcc gtcacgggat tttcgacgac
      421 gggctgcgtt gtgggggatg gctttgccgg agacagaatt tacacctgct gaacttaccg
      481 cattccggga ctactgggca gcggagggga aagtgtttac gcagattcag tgggagcaga
      541 aattcgcccg tcacgtaaat cacgtcaggg cgcaggttaa accagtcagc aagggggtaa
      601 accatgcagc agcaccaggt ggcaccgcat cacgggcagt tcaggaaatt cgggcagcac
      661 gtgagcagtg ggaacgtgaa aacggattta tcagcgacgg aaacggcctg gaagctgtgg
      721 gaactcatgg gggaggttta ttcgaaccgc tggacccaga agaacggggc cgcaccttcg
      781 aagctctgga ttgcacagat tggcgcgatg actgagcagc aaatccggca ggtctgccgc
      841 cagtgcatgg accgctgccg ggcgggtgaa acatggcctc cggacctggc tgagtttgtg
      901 gcgctgattt cggaaagcgg ggccaatcca tttggcctga cggtggatgc tgtgatggag
      961 gagtatcgcc gctggcgtaa tgagtcctgg cgatataacg gaagcgacaa atatccgtgg
     1021 cctcagcctg tgctgtatca catttgcctc gagatgcgtt caaaggggat tgaacgccag
     1081 atgaccgaag gggaattaaa acggcttgca gaacggcagc tgacgaaatg ggcaaagcat
     1141 gttagtaacg gcctgagcgt tccgccagta cggcgacaac tggcggcacc aaaacgcccg
     1201 ttgggaccaa cgccaatt
//
LOCUS       DAZGCS010000353         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGCS010000353 DAZGCS010000000
VERSION     DAZGCS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.360"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..538
                     /gene="ygeG"
                     /locus_tag="ACHK01_005413"
     CDS             47..538
                     /gene="ygeG"
                     /locus_tag="ACHK01_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8100130.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            872..>1191
                     /locus_tag="ACHK01_005414"
     CDS             872..>1191
                     /locus_tag="ACHK01_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO8100131.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVF"
BASE COUNT          430 a          182 c          207 g          372 t
ORIGIN      
        1 aatcaataat aatttagcat cacgagacac atcacagagg aatattatga gcacagaaac
       61 aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacc tattatcgaa
      121 aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata gaatatatgc
      181 atatgcattc gactaccatg aaaagggtaa tgtaacggac gcagaaattt attataaatt
      241 tttgtgcatt tatgcgttcg aaaatcatga gtatctagaa ggttttgcat cagtatgtca
      301 gtcaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca attacttccc
      361 gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg ctaaaaatat
      421 cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg atagtgttaa
      481 gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata atggctaagc
      541 attacaatca tttggttaaa tttgctggtt atagtgcagt taaccactat aaccagcacc
      601 tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata aatcattatt
      661 tttcaatgcg ttagagaaca atctacttta tcaaaattga ataaggctat ttcagattac
      721 atcactgaat tcctaagata aattagtgaa cacaaccata gaatgtcgaa tgtaatttcc
      781 tgtagcgtta atgacacata aatatgaata gccataattt ctattgctta agcaggatgc
      841 aagaaaccaa ttttttcata gaggttaact aatggactta gaaaataaat tctcatatca
      901 ttttcttgag ggattaacgc tcacggaaga tggaattctt actcaaggaa atgagcaagt
      961 ttatattcca cagaaagagt taggtgtatt aatagtatta cttgaatccg ctggtcatgt
     1021 tgtactgaaa gatatgatca tcgaatcagt atggaaaaat attattgtta gtgacgagtc
     1081 cctgacaaga tgtatctatt ctttgcgctg catttttgaa aaaattggct atgatcgttg
     1141 catagaaaca atctaccgga aaggttatcg tttcagcggg caggttttca a
//
LOCUS       DAZGCS010000354         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGCS010000354 DAZGCS010000000
VERSION     DAZGCS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.361"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1190
                     /locus_tag="ACHK01_005415"
     CDS             <1..>1190
                     /locus_tag="ACHK01_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8100132.1"
                     /translation="RRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSG
                     TLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTT
                     AITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPAGVELTPGYFQ
                     ITAVPKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVSGPHIKPGKDFW
                     FYVRSVNLVGKSAFVEASGRASNDAAGYLELFREKIGKTHLAEALWAEIDNSQLKDEM
                     AEMQTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGI
                     PYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKR
                     LFAV"
BASE COUNT          292 a          307 c          368 g          223 t
ORIGIN      
        1 cgtcgcctgt tccgctgtgt ttccatccgg gaaaacacgg acggcacctt tgccatcacg
       61 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggggcgca ctttgacggc
      121 gaccagagcg gcaccctgaa cagcgtcatc cctccggcag tgcagcacct gacggtggag
      181 gtgagcgcgg ctgacggtca gtatctggca caggcgaaat gggacacgcc gcgggtggtg
      241 aagggtgtgc gcttcagtct gcgcctgacc agcggaagcg gagaagacag ccgtctggtg
      301 accaccgcca tcactgcgga tacagagcat cgtttcagtg gtctgccgct cggggaatac
      361 accctgacag tcagggcaat taacagttat ggccagcagg gcgaaccggc caccaccacc
      421 ttccggatta acgcaccggc agcgcctgcc ggtgttgaac tgacgccggg gtattttcag
      481 ataacggcag tcccgaaact gaccatttat gacccgacgg tgcagtttga gttctggttt
      541 tcggaggcaa aaattgcaga cgccgcacag gtggaaacct ctgcccgtta tctggggacc
      601 ggcagtcagt ggagtgtctc cggtccgcac attaagcccg gaaaggattt ctggttttat
      661 gtgcgcagcg tcaacctggt gggtaaatct gcttttgtgg aagccagtgg acgggcgagc
      721 aatgatgctg cgggctatct ggaacttttc cgggaaaaga taggaaaaac gcatctggca
      781 gaggcgctgt gggcagagat tgacaacagt cagctgaagg acgagatggc ggaaatgcag
      841 accaccatca cagaaacccg caatgaaatc acacagacgg tcagtaaaac gctggaagac
      901 cagagcgcca ccattcagca gatacagcgc gtgcagaagg acacaaatga tgacctggct
      961 gcgctgtaca tgctgaaggt tcaaaaaacg aaagacggca ttccctatgt ggccgggatt
     1021 ggtgcaggga ttgaggatac tgatggccag ccactgagca acatactgct gctggctgac
     1081 cgtatcgcga tgataaatcc ggagagcggc aacagcacgc cgttatttgt ggcgcagggg
     1141 aatcagctgt tcatgaacga cgtgttcctg aaacgactgt ttgcggtgag
//
LOCUS       DAZGCS010000355         1181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGCS010000355 DAZGCS010000000
VERSION     DAZGCS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.362"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..585
                     /locus_tag="ACHK01_005416"
                     /pseudo
     CDS             1..585
                     /locus_tag="ACHK01_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(604..1106)
                     /locus_tag="ACHK01_005417"
                     /pseudo
     CDS             complement(604..1106)
                     /locus_tag="ACHK01_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          374 a          180 c          227 g          400 t
ORIGIN      
        1 gatgggataa ttggatttct cggacattat cctgatttat ataaggttgt tgggggctat
       61 aaagatctga agaaagctat agcggagtgt aacaaatcta cagcctaaat atttatttta
      121 ggagaatttg caggtggaat gatggggtcc gaactcgtaa aatgggttaa atcgcataag
      181 atagatgctc atattattac atttgtagca aagatgccgt atattgattc aataaaattg
      241 cttgaagcag gtgcgaaagg atgcgtatgg aaaaccagtc acccggcgaa actaaatcgt
      301 gctattgatt cgattagtaa tggttacact tattttgata gtgtacatat ggattgtgaa
      361 aaaatatctt ccaggtattc ctctgataat caacttacaa atcgtgagtc agaaatatta
      421 caactcatag ctgatggtaa aacaaacaaa gaaatcgcta atttcttgca gttaagcaga
      481 aaaacagtcg aaactcatag actcaatatc atgaagaaat tagatgttca cagtggtatc
      541 gagttgatca aaacagcttt acgtatgggt gtttgcacta tataaaaaaa agaggcttta
      601 ggctcatttt gctgaagcct tacgattgtc tagttcatta agcctggtat atacaatata
      661 tattttgggg catattgctg tctctttttt tggttatcca ttgcaaaacc cgcattataa
      721 gtccccaggc attgccacgt tactccacaa cgtgaaaaat ggttatataa aatctcggtt
      781 cctattttaa tgttcagaca agggttatct aacaaatctc gttcatcttt tattattcca
      841 cgttttttta acaggggaat atgcatctgg tttatctgca ttaaccaata atctaaactc
      901 tttatattgt tattcttatc tctgttcttg cctatgctat ccttttttag attgctttca
      961 acaagagcga tagctttaat taaatttggt tcgattctga acattgttcc tgcttcatta
     1021 gagcaagagt tgctggcgaa agacattttt gtaataaata tacaaaacgt gaagatggtt
     1081 aagaatttaa cttttggtga gcgcatattt tttcatgtgt gaggattgaa tgaaagggac
     1141 gagatactat tataaaagta tctcgttatg atttatttgg t
//
LOCUS       DAZGCS010000356         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGCS010000356 DAZGCS010000000
VERSION     DAZGCS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.363"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..174
                     /locus_tag="ACHK01_005418"
     CDS             <1..174
                     /locus_tag="ACHK01_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100133.1"
                     /translation="YCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GICNDLAAASTFV"
     gene            138..713
                     /locus_tag="ACHK01_005419"
     CDS             138..713
                     /locus_tag="ACHK01_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8100134.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPVFRQKLSDGWYQVRIAG
                     RDAGETELSSRLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIATAIWMPGISIA
                     FSDILFSMGAAMTLGGVAQMLAPKASTATTTSTDNGKQNTYFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVISQEISTADEGDGGEIVVIGR"
     gene            774..>1177
                     /locus_tag="ACHK01_005420"
     CDS             774..>1177
                     /locus_tag="ACHK01_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8100135.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEV"
BASE COUNT          271 a          285 c          380 g          241 t
ORIGIN      
        1 tactgtggtg acggcgagct gctgcaccat attcctgaac aactgagcaa acgagagagg
       61 tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc atggcgcgca
      121 tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt gtgaaaacgg
      181 gggctgaagc catccgcgca ctggccacac agctcccggt gtttcgtcag aaactgagcg
      241 acggctggta tcaggtacgg attgccgggc gtgatgcagg cgaaaccgaa ttatcatccc
      301 gtcttaatga gccgctggca aatggtgcag tgatccacat cgtgccgcgt cttgcgggag
      361 caaaaagtgg cggtgtgttt caggcggtgc tgggtgcggc gttgattgct acggcaatct
      421 ggatgccggg gatcagtatc gctttcagtg acattctctt ttcaatgggt gcggcgatga
      481 cgcttggcgg tgttgcacag atgctggcac cgaaagcgag taccgcaaca acgaccagca
      541 cggataacgg taagcagaac acgtatttct catcactgga taacatggtt gcccagggca
      601 atgttctgcc tgttctgtac ggtgaaatgc gtgtggggtc acgggtaatt tcgcaggaaa
      661 tcagcacggc agacgaaggg gatggtggtg aaatcgtggt gattggtcgc tgatgcagaa
      721 tgttttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta
      781 aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaag tccacgcagc
      841 tgctgagtgt gatcgatgcc atcagcgaag ggccgataga aggtccggtg gatggattaa
      901 aaagcgtgct gctgaacagt acgccggtgc tggacagtga ggagaatacc aatatctccg
      961 gcgtcacggt ggtgttccgg tcaggtgagc aggagcagac accgccggag gggtttgaat
     1021 cctccggctc cgagacggtg ctgggtacgg aagtgaaata tgacacgccg ataacccgga
     1081 ccatcacgtc ggcaaacatt gaccgtctgc gcctgacctt cggtgtgcag gcactggtgg
     1141 aaaccacctc aaagggggac aggaatccgt cggaagt
//
LOCUS       DAZGCS010000357         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGCS010000357 DAZGCS010000000
VERSION     DAZGCS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.364"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..562
                     /gene="escR"
                     /locus_tag="ACHK01_005421"
     CDS             <1..562
                     /gene="escR"
                     /locus_tag="ACHK01_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO8100136.1"
                     /translation="TSFLKISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMSPII
                     LQINDNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKENVEFFERAAQKKLGNE
                     TILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
     gene            562..831
                     /gene="escS"
                     /locus_tag="ACHK01_005422"
     CDS             562..831
                     /gene="escS"
                     /locus_tag="ACHK01_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO8100137.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            831..>1149
                     /gene="escT"
                     /locus_tag="ACHK01_005423"
     CDS             831..>1149
                     /gene="escT"
                     /locus_tag="ACHK01_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO8100138.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGS"
BASE COUNT          345 a          189 c          211 g          404 t
ORIGIN      
        1 tacttcattc ctgaaaattt cgattgtatt ggggattctg aagaacgcat taggtattca
       61 acaggtacca ccaaatatgg cgctaacatc agtatctttg atactgacaa tgtttattat
      121 gtctccgata atattacaga taaatgataa tatttctcag gaaccaatca attacaccga
      181 ctctgatttt tttcaaaaag ttgatgaaaa aatattatca ccataccgcg gttttttaga
      241 aaaaaataca gagaaagaaa atgttgagtt ttttgaacgt gcagctcaaa aaaaattggg
      301 taatgaaact atcttaaaaa aagactctct atttatactg ttaccagctt tcacgatggg
      361 gcagcttgaa gctgcattca agataggttt tttgctctat ttacctttta ttgcgataga
      421 tttgatcatt tccaatatct tattggcctt gggtatgatg atggtatcgc cagtaacaat
      481 ttcgattcct tttaagatct tgttattcat tcttgttgga ggctggcaaa aattattcga
      541 atttctgttg gtggtgaatt gatggaaact ggatattttg ttcaattatg tgtgcaaacg
      601 ctttggataa tatttatcct ctcactgcct acagtcatag cggcctctgt tgtcggcatt
      661 attattagcc tggttcaggc tataacgcag ttacaggatc aaacattgcc ttttttgcta
      721 aaaataatag cagtgtttgc tacgcttgcc ctgacttatc actggatggg aacaacaatc
      781 atcaacttct cgtcgataat atttgaaatg attccgaagg tgaatggcta atgaatgaaa
      841 taatgacggt catagtatca tcattttttt gtatactcag gccgctgggg atgtttatta
      901 ttctaccaat attttcaaca ggtgttttgc tcagtaactt tatacgcaac tcaattatga
      961 ttgcgtttac acttccaatt atagtagaga actatacatt ttcggaaaaa ttaccatccg
     1021 gaatttttca gttgacaggc atagtgctaa aagaaataag tataggcttt ttcattgggt
     1081 tatcatttac tattcttttt tgggcaatag atgcggctgg gcagattatt gatactttaa
     1141 gaggttcaa
//
LOCUS       DAZGCS010000358         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGCS010000358 DAZGCS010000000
VERSION     DAZGCS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.365"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..836
                     /locus_tag="ACHK01_005424"
     CDS             <1..836
                     /locus_tag="ACHK01_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFO8100139.1"
                     /translation="DLSRAIIEKLKHNANNESRVAQLQGGSVVFIHYKSTEPEDLGKL
                     LIVMVDKQSAYDFDSDKLTPTRLNPINTDALRQAAMFDLTLFEASYPENKGDSYVHFL
                     QGKSKSDFFKDSLGCRHDSDNKRSIQQLFSAIDIFASKNSLGRVLRDTIDNEVKSLLE
                     KKSKDKHGNKSVKIEDISKIIDSCLTDSHKCKGTFVDFVNLNGFQIDPQFEPTPKAAE
                     SALTIEVADNDNNFKLKIMRGAIGDEKSNKPVILTDNKCEIVIKLSQANYDELKRYRD
                     S"
     gene            839..>1147
                     /locus_tag="ACHK01_005425"
     CDS             839..>1147
                     /locus_tag="ACHK01_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100140.1"
                     /translation="MTIADDLSRLAQIINGASSRVEGYYTVISLEESIVIVNSSEIIR
                     LLQSIGYKKATTCIENNEIWLDRQASSWDDAIIYENVESFWSRVNTQNALPKNYIIGT
                     P"
BASE COUNT          406 a          195 c          224 g          322 t
ORIGIN      
        1 ttgatttatc tagagctata atagagaagc ttaaacataa tgctaataac gaatcgagag
       61 ttgcccaact tcaggggggg agcgttgttt tcatccacta taagtctacc gaaccagagg
      121 atttggggaa acttctaatc gtaatggttg ataaacaaag cgcctatgac tttgattcgg
      181 ataagttgac cccaacaaga ttaaatccaa ttaataccga tgccttacgg caggctgcga
      241 tgttcgattt aactttattc gaagccagtt atccagaaaa caagggtgac tcatatgtac
      301 atttcttgca aggtaaatct aaaagcgatt ttttcaaaga ctcattaggt tgccgacatg
      361 attcggacaa taaaagaagc attcagcaat tattcagtgc tatagatatt tttgctagta
      421 aaaactcact cggtcgtgta ctgcgtgata ctattgacaa tgaagttaaa tccttactgg
      481 aaaaaaaatc aaaagataaa cacggaaata agtccgttaa gatagaggat atttcaaaaa
      541 taattgactc atgcctgact gattcacaca aatgtaaggg aacgtttgtc gattttgtta
      601 atcttaatgg tttccagatt gaccctcaat ttgaaccaac tcctaaagca gctgaaagcg
      661 cgcttacaat tgaagtggca gataacgaca ataattttaa gttgaaaata atgcgtggag
      721 ctattggaga cgaaaagtca aataagcctg taattctcac tgacaacaaa tgcgaaattg
      781 tgataaaatt gagtcaggca aattatgatg aactcaaaag atatagagac agctaataat
      841 gacaattgct gatgacttat caagattagc gcagattatt aacggagcct caagcagagt
      901 tgagggctac tacactgtca taagtcttga agaaagcatt gttattgtaa atagttctga
      961 aataattaga ctgttacaat ctataggtta taaaaaggca acaacctgca tcgaaaataa
     1021 tgaaatttgg ctagatcgcc aagcttcatc ttgggatgac gctataattt atgagaatgt
     1081 tgagtctttt tggtctagag taaacaccca aaacgctctt ccgaaaaatt atatcatcgg
     1141 aacgccg
//
LOCUS       DAZGCS010000359         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGCS010000359 DAZGCS010000000
VERSION     DAZGCS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.366"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..508
                     /locus_tag="ACHK01_005426"
     CDS             53..508
                     /locus_tag="ACHK01_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO8100141.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFME
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            609..817
                     /locus_tag="ACHK01_005427"
                     /pseudo
     CDS             609..817
                     /locus_tag="ACHK01_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            918..>1147
                     /locus_tag="ACHK01_005428"
     CDS             918..>1147
                     /locus_tag="ACHK01_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8100142.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGR"
BASE COUNT          302 a          301 c          279 g          265 t
ORIGIN      
        1 tttatcacct gatttgtcag tgtcatcgtc atgactcaga gaggtaaata ccatgattca
       61 cctgttcaaa acctgcatga ttaccgcctt cattctgggg ttaacgtggt ctgccccact
      121 ccgggcacag gatcaacgtt acatcagtat acgcaataca gatacgatat ggctcccggg
      181 aaatatttgt gcctaccagt tccggctgga taatggcgga aacgatgaag gatttggccc
      241 cctcaccatc actctgcaac tcaaagacaa atatggtcag acgctggtga ccagaaaaat
      301 ggaaacggaa gcctttggtg acagtaatgc cacgcgaacc acagacgcat ttatggaaac
      361 ggagtgcgtg gaaaatgtcg ccacaaccga aatcattaaa gcaactgaag aaagtaacgg
      421 ccatcgtgtc agtctgccgt tatcggtttt cgatccccag gactaccatc cactgctgat
      481 taccgtttcc ggaaaaaatg ttaactgatc cctgaagtca gtttcatgct tcagcctctg
      541 ccacaacgta tggcagaggc tatttatcag gagaaaccag atatgccggg ttacacacag
      601 agtatgtcct ggctgaagga tctttctcat acgggcagac ggtggctgtc acaacagctt
      661 accttaatgt gcttattgaa gatgaccagg tgcgcacttc cggctggtta tccgcaacgc
      721 ggaaggtcag cgacgctggc gctgccggaa tactgaaccc tgtgccggaa agcagcttaa
      781 tgcgtggctc gccagtgaag ggcttctcag acaataaacg tcattgattt cgtccatcag
      841 gccgcgtctt ctccgggaga cgcggctttt ttcatttatt acaccagtaa atcttaacca
      901 ccgataagga gcaaagtatg cgattagcca gtcgttttgg ttatgctgca aaccagatac
      961 gccgtgaccg tccactgaca catgaagagc tgatgcacca tgtgccgggg attttcgggg
     1021 aagataaaca cacgtcccga agccagaact atacgtacat ccccaccatc accgtgctgg
     1081 aaagtctgca gcgggaaggc ttccagccat tcttcgcctg ccagacccgt gtgcgcgacc
     1141 cgggccg
//
LOCUS       DAZGCS010000360         1142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGCS010000360 DAZGCS010000000
VERSION     DAZGCS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.367"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..295)
                     /locus_tag="ACHK01_005429"
     CDS             complement(<1..295)
                     /locus_tag="ACHK01_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO8100143.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQ"
     gene            complement(368..943)
                     /locus_tag="ACHK01_005430"
     CDS             complement(368..943)
                     /locus_tag="ACHK01_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO8100144.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMFSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(1056..>1142)
                     /locus_tag="ACHK01_005431"
     CDS             complement(1056..>1142)
                     /locus_tag="ACHK01_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO8100145.1"
                     /translation="DKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          347 a          287 c          193 g          315 t
ORIGIN      
        1 tctgaaaata gttattaaca ctacttaaaa aatcgacacc tccatttaat tgcctttcca
       61 gagccacagc ccttctctcc attcgatgct cacgccccaa tagcctatcc agtaagcctc
      121 ctctcaccgg ttcaactgaa aatccatcca gtagctggac atagtgaaca gaatattggc
      181 cactaccaat ggatattctt attccacttt caagtctggc agcatcctgc aaagactgta
      241 ctcctgcagg agataattga gatatacttg ggatacttaa ggtggtaaca ggcataaaaa
      301 cctcattcag taattcatat cagtttgcag gatgtttcat taaataaaat aaagggtaat
      361 gttcatttca attacccttt ataacgaagt ttcctctggt atcgtcataa atgcattctt
      421 catgttttac aattattgat gccgttattg gttcccgggt cagtgggtgg ggtaagcctt
      481 caccagtcaa acgagaaaat gcagcggcat caaataaagt acatacatct gaaccatctg
      541 aattcttcac aaaaatacct ttttcaggct gctccagtgt aattggacat tgaatagcct
      601 caagcgggca ctgaagtctc tctggacaaa cagaaaactt acattgagat attttgttct
      661 gaatactctc ttgcgattgt gcttcacctg agtccgaaaa catacttagc attctctcac
      721 caagggctcg aggaccacta ttaaatccca aaagcaataa ctcagaaagc aatccgccat
      781 taccgccact ggatgaaaag cgcccattag tagaatcata cacaatactg acagtttcac
      841 caccgactgt aatatctccg cgcccattgg ctacaattcg ggcacgaaca acctccaccc
      901 caagattaaa tgaatgtgat ctaatatctg aggttaatgg cataacaaag ctccctattt
      961 taatttgaac tccagactta aatagctgta acaaacatcc cccatacgtg acaacaaaaa
     1021 ccagagcagg actccggttt ttgtgaaccc gtcggctatt tcatcccgcc aatattttcc
     1081 cacctcccgt cagcacgcag gatttgcagc ggtcttacca cgcactgtat ctgcttttta
     1141 tc
//
LOCUS       DAZGCS010000361         1141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGCS010000361 DAZGCS010000000
VERSION     DAZGCS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.368"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..160
                     /locus_tag="ACHK01_005432"
                     /pseudo
     CDS             72..160
                     /locus_tag="ACHK01_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(576..>1141)
                     /gene="nleF"
                     /locus_tag="ACHK01_005433"
     CDS             complement(576..>1141)
                     /gene="nleF"
                     /locus_tag="ACHK01_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO8100146.1"
                     /translation="PTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLTPELQDKL
                     DAMISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGMTI
                     DDRDGYIRELFFLYSLKVKIEESRQGKEDSKCKVYGLLCPHHSSELYGDLRAMKCLVE
                     GCSDDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT          341 a          267 c          188 g          345 t
ORIGIN      
        1 ttgctgtttt tctttctcat aaaatatttc ctgatatccc ttaacattac tgatactcaa
       61 gtagtgaatt tatgagcaat cctaatccat gcatgacgtg tggtgcctgt tgtgcatttt
      121 tcgcgtctct ttttactggg ctgaagcaga tgatgctggc gaaaggttta tcagaaaacc
      181 gtagcgcctt cttgcatacg acgtgtccgc agttttatat aatcccgacc agaaacctct
      241 tataaaaagt cgatacacaa atatcatgga taatcaccac cttctgacga agaatatctg
      301 atgaaattaa tcgcccctct cctgagacca ttgcgctggg gtaaatttgt aactacgtgt
      361 gtacagtgag gtgtcgtata gcacgccgtc aggaaatgca aggtgccccc gatatccccg
      421 actacaagtc agcaaacaaa gtgattggct ggcaatccac atcacagaaa tggacaatgc
      481 tactttataa tcgagcttgg tagccacctt gatggtcact tttaaaaaaa gcgtttacac
      541 aaaatgataa gcaggctcac aggacctccc tctaatcatc cacattgtaa agctcctttg
      601 ttgtaagtaa gatccggcac cctaataata tcaaaaggat caaaatcatc actgcatcct
      661 tccacaagac atttcattgc tcgtagatca ccatatagct ctgaagagtg atgcggacat
      721 aacagaccat aaactttaca tttagaatct tctttacctt gtctactttc ctcaattttt
      781 actttcaatg aatacagaaa aaaaagctcc ctgatataac catccctgtc atctatcgtc
      841 atcccctccc tcagtgctcc gacaacctca tcagtatttt catttctcac ctcaaataca
      901 ctatttctct tatccatcag cccacttaca aaagcactta gctcttgata aatttcctca
      961 agctcattac tatttctggc atttgagtat atagagatca tagcatcaag tttatcttgt
     1021 aattcaggag taagaaaatg attatgatta agttcactta ctgattccgg agtcgaaggg
     1081 ttacctcttc ctgatacata catccatgaa taaagatttg ctgaagagcc acttgttggt
     1141 a
//
LOCUS       DAZGCS010000362         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGCS010000362 DAZGCS010000000
VERSION     DAZGCS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.369"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            100..1074
                     /gene="cysB"
                     /locus_tag="ACHK01_005434"
     CDS             100..1074
                     /gene="cysB"
                     /locus_tag="ACHK01_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO8100147.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          292 a          267 c          269 g          302 t
ORIGIN      
        1 gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag gtgtgacgaa
       61 aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca acaacttcgc
      121 tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga aggactttac
      181 acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct aggcattcaa
      241 attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcaggaca agaaataatt
      301 cgtatcgctc gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc cggtgagcac
      361 acctggccgg ataaaggctc gctgtatatc gccaccacgc atacccaggc acgctacgcg
      421 ttaccaaacg ttatcaaagg ctttattgag cgttatcccc gcgtttcttt gcatatgcac
      481 cagggctcgc cgacacaaat tgctgatgcc gtctctaaag gcaatgctga tttcgctatc
      541 gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta ccactggaat
      601 cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat taccattgaa
      661 gaactggcgc aatatccgtt ggtgacatat accttcggct ttaccggacg ttcagaactg
      721 gatactgcct ttaaccgcgc agggttaacg ccgcgtatcg tcttcacggc aacggatgct
      781 gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc cagcatggcg
      841 gtggatccgg tcgccgatcc cgaccttgtg cgtgttgatg ctcacgatat cttcagccac
      901 agtacaacca aaattggttt tcgccgtagt actttcttgc gcagttatat gtatgatttc
      961 attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc
     1021 tctaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa ataatttctt
     1081 gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc
//
LOCUS       DAZGCS010000363         1096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGCS010000363 DAZGCS010000000
VERSION     DAZGCS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.371"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..643
                     /gene="nleB"
                     /locus_tag="ACHK01_005435"
     CDS             <1..643
                     /gene="nleB"
                     /locus_tag="ACHK01_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO8100148.1"
                     /translation="IYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            692..>1096
                     /gene="nleE"
                     /locus_tag="ACHK01_005436"
     CDS             692..>1096
                     /gene="nleE"
                     /locus_tag="ACHK01_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO8100149.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKI"
BASE COUNT          361 a          172 c          227 g          336 t
ORIGIN      
        1 aatctaccga gcagatgtgg atttcagcat gtatgataaa aaactatctg atatttacct
       61 tgaaaatatc cataaacaag aatcataccc tgccagtgag agggataatt atctgttagg
      121 cttattaaga gaagagttaa aaaatatccc agaaggtaag gactctttga ttgagtcata
      181 tgcagaaaaa agagaacata cttggtttga ttttttcagg aatttggcca tgttgaaggc
      241 tggaactttg tttacagaga cgggaaaaac tggatgccat aacatatcgc cctgtagcgg
      301 atgtatatat cttgatgccg acatgattat taccgataaa ttaggagtcc tgtatgctcc
      361 tgatggtatc gctgtgcatg tagattgtaa tgatgagata aaaagtcttg aaaatggtgc
      421 gatagttgtc aatcgtagta atcatccagc attacttgca ggcctcgata ttatgaagag
      481 taaagttgac gctcatccat attatgatgg tctaggaaag ggtatcaagc ggcattttaa
      541 ctattcatcg ttacacgatt ataatgcttt ttgtgatttt attgaattta agcatgaaaa
      601 tattataccg aataccagta tgtataccag cagttcatgg taagtcttcc ggttgtggtt
      661 gaaaaatatc gttctttagg tatagattca gatgattaat cctgttacta atactcaggg
      721 cgtgtcccct ataaatacta aatatgctga acatgtggtg aaaaatattt acccggaaat
      781 taaacatgat tactttaatg aatcacccaa tatatatgat aagaagtata tatccggtat
      841 aaccagagga gtagctgaac taaaacagga agaatttgtt aacgagaaag ccagacggtt
      901 ttcttatatg aagactatgt attctgtatg tccagaagcg tttgaaccta tttccagaaa
      961 tgaagccagt acaccggaag gaagctggct aacagttata tccggaaaac gcccaatggg
     1021 gcagttttct gtagatagtt tatacaatcc tgatttacat gcattatgtg agcttccgga
     1081 catttgttgt aagatc
//
LOCUS       DAZGCS010000364         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGCS010000364 DAZGCS010000000
VERSION     DAZGCS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.372"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..830
                     /gene="gadX"
                     /locus_tag="ACHK01_005437"
     CDS             6..830
                     /gene="gadX"
                     /locus_tag="ACHK01_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO8100150.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          322 a          203 c          229 g          323 t
ORIGIN      
        1 caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat aaatatattc
       61 tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt tttgcggatg
      121 caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca agggatacgc
      181 tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca cataaaaaaa
      241 tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag gaagttgcgc
      301 aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga gcattaatct
      361 ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt ttgaacgttt
      421 tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc gcccatgagt
      481 ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta aagaaaaaat
      541 tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg caacgtgctt
      601 tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc tgtggatatc
      661 acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg cccacagagt
      721 atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt attgttgcgc
      781 aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag aattttactc
      841 agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg tctccagact
      901 aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca gtaagttaaa
      961 tataactttt actggaaata agatcagcca tttttttata aacataagct atacgcagtg
     1021 cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca aaactac
//
LOCUS       DAZGCS010000365         1073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGCS010000365 DAZGCS010000000
VERSION     DAZGCS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.373"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1073
                     /locus_tag="ACHK01_005438"
     CDS             <1..>1073
                     /locus_tag="ACHK01_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100151.1"
                     /translation="VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETAL
                     GVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLQRNGNWV
                     TEKDVTINGKTTSQFLASVILENLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEII
                     DVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSL
                     KPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGFGGTEPR
                     MTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDAVWPYTNSDVVVDD
                     NGVGFRYSFSALKDRHTAVEVNY"
BASE COUNT          236 a          296 c          348 g          193 t
ORIGIN      
        1 ggtgaacaaa accccgctga cggacacgga cggtaatcct gtgatacatg gtgtgacagc
       61 ggtctggcgc gccggggagc aggagcagac accgccggaa ggttttgagt catccggctc
      121 tgaaaccgca ctgggcgtgg aagtgacgaa ggcaaagccg gtgacgcgca ccattacgtc
      181 cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag tcactggtgg agaccacctc
      241 aaagggtgac cgtaacccga cgtctgtccg cctgctgatt cagctgcagc gtaacggtaa
      301 ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc acctcgcagt ttctggcgtc
      361 ggtgattctg gagaatctgc ctccccgtcc ctttaacatc cggatggtcc gggagacggc
      421 ggacagcacc acggaccagc tgcagaataa gacgctctgg tcgtcataca ccgaaatcat
      481 cgatgtgaaa cagtgctacc cgaacacggc cattgtgggg ctgcaggtgg atgcggagca
      541 gttcggcggc cagcagatga cggtgaacta ccatatccgc ggtcgcatca tccaggtgcc
      601 gtcaaactat gacccggaaa aacgcacgta cagcggtatc tgggacggca gtctgaaacc
      661 ggcatacagc aacaatccgg cctggtgtct gtgggacatg ctgactcacc cgcgctacgg
      721 gatggggaaa cgcctggggg ccgcggacgt ggacaaatgg gcgctgtatg ccatcgggca
      781 gtactgtgac cagcgtgtcc cggacggctt cggagggaca gagccgcgga tgacctttaa
      841 tgcgtacctg tcacaacagc gtaaggcgtg ggacgttctc agtgatttct gctcggcgat
      901 gcgctgtatg ccggtatgga acggccagac gctgacgttc gttcaggacc gcccgtcgga
      961 tgcggtgtgg ccgtacacca acagcgatgt ggtggtggat gataacggcg tggggttccg
     1021 ctacagcttc agcgccctga aggaccgcca cacggcggtg gaggtgaatt aca
//
LOCUS       DAZGCS010000366         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGCS010000366 DAZGCS010000000
VERSION     DAZGCS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.374"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHK01_005439"
     CDS             <1..759
                     /locus_tag="ACHK01_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8100152.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          252 a          272 c          256 g          288 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgacacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tacttttgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt tgtaaaaacc
      781 gaatgcccag cctttaaaaa aacagctggg cattcggttg cttattaatc gcaataatat
      841 attggctgtc tattcatcgt gttgataaga tatatgttat atgaatgttt attaaatgta
      901 gcgattgcct ttaatgtcac gaaaagggat tatttgcttg ctattaattt cactgcgttc
      961 ttaaacacgt ttttgtatta taaatccagt catcctgccc gtagaacatc acacattatc
     1021 atcctgttct cccgcttcgt ttaaatttat ttatcaatca atttgact
//
LOCUS       DAZGCS010000367         1050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGCS010000367 DAZGCS010000000
VERSION     DAZGCS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.375"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            216..425
                     /locus_tag="ACHK01_005440"
     CDS             216..425
                     /locus_tag="ACHK01_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100153.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(426..>1050)
                     /locus_tag="ACHK01_005441"
     CDS             complement(426..>1050)
                     /locus_tag="ACHK01_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100154.1"
                     /translation="LPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRDAFTGL
                     YFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAGNMF
                     VYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAEKRWNV
                     ATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          290 a          235 c          208 g          317 t
ORIGIN      
        1 tgccacaacc ggaagcgcac gttcgaagaa atctaacgac aagccttcta agggaaagag
       61 cttcgccgta cgctttcgcg ttatgcactg acttttcagg gaaatatcct ttcagtaaac
      121 tgtcagtacc ggattcttat ccgtgtccgg cgcacgacca cacgtgacag cgtgttggtc
      181 tccattttta acccagaacc tcaatggagg ataaaatgcc aaacaaaaaa agaaatccgc
      241 ttattgaaaa acagattgaa tgcctggtaa atcaactcag gcaatcaggg ttattaaaaa
      301 ctcattcaga gttgaggctc acagaatcag cattcgacga taaattaaat aatgtccttt
      361 ataatggcat tattgatttt aatcgttctg ttggtcgccg cggccctgct ggtgtttcct
      421 tataattacc agtcaatcca gagtggaccg tgttcagcgt aaatataact gtacacatcc
      481 agattatatt tgtggtctgt taagaacagg ccgcaaatac atgccgaagc ttccagtgca
      541 gcggctctgt tactgaataa ccatgtagca acattccagc gtttttctgc atcccagtct
      601 ttatcaaggc ctgataccat gaagaaaccg ttagtgttgc catcaaataa ttctgtttcc
      661 aaatttttaa gcaatgcctg atggactctt gccaggtatt ccgccggaat ttcgccacga
      721 attctgatga gattgtcata aacaaacatg ttccccgcat atggcgattt ttctttcttg
      781 ttttttaaac cagcatcatg agcaaactga tcaatttctt cttccgttgg tttcgtattg
      841 atgttttgcg ctgtcgtttc tgcaatttta tttgccacac tctctgagtc gtgtttattt
      901 atagacgcac agaaatacaa tccggtaaac gcatcgcgca cattacgagc catattatca
      961 gtgtcttttt tcgttaccga ttccaattca agttcgttca gacgatgacg aagtgtgtgt
     1021 gctgcaatct cctggattga aggaggtaaa
//
LOCUS       DAZGCS010000368         1037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGCS010000368 DAZGCS010000000
VERSION     DAZGCS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.376"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..952)
                     /locus_tag="ACHK01_005442"
     CDS             complement(137..952)
                     /locus_tag="ACHK01_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFO8100155.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          307 a          240 c          212 g          278 t
ORIGIN      
        1 atttatgagt aaaggattat gtccacgata agcacctggg tcgattcctg ggaggcggcc
       61 atgagggtag ggaagcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
      121 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      181 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      241 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      301 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      361 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
      421 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
      481 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
      541 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      601 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
      661 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      721 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      781 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
      841 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
      901 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
      961 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1021 gtgcaatgta acatcag
//
LOCUS       DAZGCS010000369         1029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGCS010000369 DAZGCS010000000
VERSION     DAZGCS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.377"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1013
                     /locus_tag="ACHK01_005443"
     CDS             <1..1013
                     /locus_tag="ACHK01_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8100156.1"
                     /translation="FPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCND
                     HISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQ
                     FIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTLESIYRAMTIFD
                     RLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKI
                     LAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKS
                     LQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRIC
                     MR"
BASE COUNT          377 a          187 c          170 g          295 t
ORIGIN      
        1 tattcccttt cactacttcg ttgaatacac tggatccatt aatacttaat caggaattag
       61 tgcaaatcat ttcaaataaa aaaatcgatg gtctctatac ctatccgatg gctgcgacaa
      121 atttttgtaa tgatcacata tctcaaaatt cattcttgag cagattcaag ccagattatt
      181 tcgttacagg aagaataaac cagaataatg cagtgaacac tttatacatt gagttgatcg
      241 acgctaaaaa ccttttcctc atcgccagta atcatctccc tgttgatgaa ctacataata
      301 catcacaatt tattatagat aatatccttc aaacggtaca taaaccagaa cgatctgtaa
      361 gattagctaa gcaggaccaa ggatataaga atcattattt atcagatgaa atgttagccg
      421 gaaagaaaga actttacgac ttcacccttg aaagcattta cagggccatg actatatttg
      481 atagattaca aaataaaagt gatatacaga cgctaaaaac agaatgttat tgccttctag
      541 cggaatgcca tatgtctttg gcacttcatg gaaaaagtga acttgaactt gctgctcaaa
      601 aagcattaga gcttttagat tatgtatcag acataaccac tgtcgatgga aaaattttag
      661 ctattatggg actgataact ggtctgtctg gacaagcaaa agtatctcat atcttatttg
      721 aacaggctaa gatacactca actgatatag cctctctcta ctactatagg gcactagtcc
      781 actttcataa tgaaaaaatt gaagaggcaa ggatttgtat agacaaatca ctacaactcg
      841 aacccagaag acgaaaagca gttgtgataa aagaatgtgt agatatgtat gtgcctaacc
      901 cgctcaaaaa caacattaaa ctctactata aagaaactga gagtgaaagc catcgagtta
      961 taattgacaa cattttgaaa ttaaagcagc tgacgagaat ttgtatgcga taaattttta
     1021 ttgacgcaa
//
LOCUS       DAZGCS010000370         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGCS010000370 DAZGCS010000000
VERSION     DAZGCS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.378"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..328
                     /locus_tag="ACHK01_005444"
     CDS             <1..328
                     /locus_tag="ACHK01_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8100157.1"
                     /translation="ADPEQELSDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIM
                     LANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLS
                     INKLSQ"
     gene            333..>1026
                     /locus_tag="ACHK01_005445"
     CDS             333..>1026
                     /locus_tag="ACHK01_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8100158.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHAS"
BASE COUNT          207 a          271 c          336 g          212 t
ORIGIN      
        1 agcggacccg gagcaggagc tgagcgaccg ctgggtggtg gagcagatgt cagagctgac
       61 ggccatgaca gcctcgtttg tgctggcaac accgacggag acggacgggg cgctgtttcc
      121 tggtcgcatc atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta
      181 taacggtcct gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag
      241 atgcagcaag tgcatgcgcg ggtgtgagat gcgcggcatg gtggctaatt ttggcggttt
      301 cctttccatt aataaacttt cgcagtaaat ccatgacaga gacagaatca gcgattctgg
      361 cgcatgcccg gcggtgtgcg cctgcggagt cttgcggctt cgtgataagc acgccggagg
      421 gtgaccggta ccagccgtgc gtgaatatct ctgcagagcc tgaggcatat tttcgtattg
      481 ccccggaaga ctggctgcgg gcagagatgc agggggagat tgtggcactg gtccacagcc
      541 atcccggtgg cctgccctgg ctgagcgagg ccgaccggcg gctgcagata aaaagtgccc
      601 tgccctggtg gctggtcagc cggggtgaca ttcacaaatt ccgctgcgtt ccgcacctga
      661 ccggacggcg ctttgaacac ggggtgacgg actgttacac cctgttccgg gatgcgtacc
      721 atctggcggg gactgaaatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc
      781 agaacctgta cctggacaat atggagggaa cgggctttta ccgggtgccc ctgtcctctg
      841 cacaggcggg cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccgcca
      901 tttactgtgg caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga
      961 ggtattccga aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg
     1021 catctg
//
LOCUS       DAZGCS010000371         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGCS010000371 DAZGCS010000000
VERSION     DAZGCS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.379"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..97
                     /locus_tag="ACHK01_005446"
     CDS             <1..97
                     /locus_tag="ACHK01_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8100159.1"
                     /translation="NPTASQNRSPAPVFVVMSGEYLLDKKEEIIQ"
     gene            110..199
                     /locus_tag="ACHK01_005447"
     CDS             110..199
                     /locus_tag="ACHK01_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100160.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            219..>1016
                     /gene="nleL"
                     /locus_tag="ACHK01_005448"
     CDS             219..>1016
                     /gene="nleL"
                     /locus_tag="ACHK01_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO8100161.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNC"
BASE COUNT          302 a          167 c          217 g          330 t
ORIGIN      
        1 taacccgaca gcttcacaaa accggagtcc ggctccggtt tttgttgtca tgtccgggga
       61 atatttgtta gataaaaaag aggagataat tcaataggga gttaaattaa tgccgataaa
      121 tctgacatct tatttgtggg tacagggaca gaaagttgtc ccggcagttg tgttttctaa
      181 ttttacttta gtggactaag taaaaaggag tgagataaat gctgcccact acaaatatct
      241 ctgtaaattc tggagtaata tcttttgaaa gtcctgtaga ttcaccatct aacgaggatg
      301 ttgaagttgc cctcgaaaag tggtgcgctg agggagaatt tagcgaaaat cgtcatgagg
      361 ttgcatcaaa aatacttgat gttataagta ctaatggaga gactttatca atcagtgagc
      421 caataacaac attaccagac ttgcttccag gttctctgaa agaactggtt ttgaatggat
      481 gtacagagct taaatcaata aactgcttac cccccaactt atcttcatta agtatggttg
      541 gatgctcatc attagaggtt ataaattgca gcatacctga aaatgtcatt aatttatctt
      601 tatgccattg tagttctttg aaacatatag aaggttcctt tcctgaggca ctcagaaatt
      661 ccgtatattt aaatggctgt aattcattaa atgaatcgca atgtcaattc cttgcatatg
      721 atgtcagtca aggccgtgcc tgcctgagca aagctgagct tactgctgac ttaatttggt
      781 tgtcggctaa ccgaacgggt gaagagtctg ctgaagaatt gaattactct ggatgtgact
      841 tgtcaggtct aagtcttgta gggctgaatt tatcatcagt aaatttttct ggagcagtgc
      901 ttgatgatac agatctcagg atgagtgatt tgtctcaggc tgtattggaa aactgttctt
      961 ttaaaaactc gattttgaat gaatgtaatt tttgttatgc taatttatct aattgt
//
LOCUS       DAZGCS010000372         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGCS010000372 DAZGCS010000000
VERSION     DAZGCS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.380"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1005
                     /locus_tag="ACHK01_005449"
     CDS             <1..>1005
                     /locus_tag="ACHK01_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8100162.1"
                     /translation="AGTITVYEDSQPGTLNDFLGAMSEDDVRPEALRRFELMVEEVAR
                     HAEEAKKNAGEAETSARNAGISASQAEEGAANADTSAGEASESARQAAESAAAAKQSE
                     EASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTATEKARESAESAQS
                     AEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPVGATGERGPA
                     GDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVG
                     ATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPDTDGALITGLTFLDPKDATRVQG
                     "
BASE COUNT          251 a          264 c          363 g          127 t
ORIGIN      
        1 atgccgggac catcaccgtg tatgaagatt ctcaaccggg gacgctgaat gattttctcg
       61 gtgccatgtc ggaggatgac gtccggccgg aggcactgcg ccgttttgaa ctgatggtgg
      121 aagaggtggc gcgtcacgca gaggaggcga agaagaatgc cggagaggcg gagacgtcag
      181 cgaggaatgc cggcatatca gccagtcagg cagaagaggg cgctgcaaat gctgacactt
      241 cagcagggga ggcatcggag tcagcccggc aggcggcaga aagtgcagcc gctgcaaagc
      301 agtcagagga agcgtcctcg tcctcggcct ctgcggccgc tcaaaaagcc agtgagtcat
      361 cacaaagtgc agcagaagct gaattgtcaa gaaagacggc agaaagtgca gccggtaatg
      421 cagccaggga tgcaacgacc gcaacagaaa aagcccggga gtcagcagaa agcgcacagt
      481 cagcggaaca aagcaggata gcggcggaag aggccgtaaa ccgaatcccc accgtggtgg
      541 ggcctcccgg gccaaagggg gaaccgggtc ccgcgggtcc tcaggggccg aagggtgata
      601 agggagagcg cggtgacacc ggccctgtcg gggcaaccgg cgaacgggga ccggcaggtg
      661 atgctggtcc ggcaggccca cagggaccga aaggcgacag gggagagcgg ggagagaccg
      721 gtctgacggg aaatgcaggt ccacagggtc caaagggaga taccggtgcg gcaggcccgg
      781 ctggcccaca gggaccgaaa ggagaaacag gtgcggctgg cccggtgggg gcaaccggac
      841 ctcagggacc gaagggcgac ccgggggaga cacaaatccg ttttcgtctg gggccggcga
      901 gcattattga gacaaacagc catggctggt tcccggatac agatggcgca ctcatcaccg
      961 gactgacctt tcttgacccc aaagatgcca cacgggttca gggtt
//
LOCUS       DAZGCS010000373          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGCS010000373 DAZGCS010000000
VERSION     DAZGCS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.381"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /locus_tag="ACHK01_005450"
     CDS             complement(<1..607)
                     /locus_tag="ACHK01_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8100163.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAASAKQSEEASSSSASAAAQKASESLQSATDAELSKK"
     gene            complement(672..>995)
                     /locus_tag="ACHK01_005451"
     CDS             complement(672..>995)
                     /locus_tag="ACHK01_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8100164.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDGRHSNTSLAWGAGVQVNPTESVAIDIAYECSGSGDWRTDGFIVG
                     VGYKF"
BASE COUNT          198 a          320 c          236 g          241 t
ORIGIN      
        1 tcttttttga caactcagca tctgttgcac tttgtaatga ctcactggct ttttgagcgg
       61 ccgcagaggc cgaggacgag gacgcttcct ctgactgctt tgctgaggct gcactttctg
      121 ccgcctgccg ggctgactcc gatgcatccc ctgctgaagt gtcagcattt gcagcgctct
      181 cttctgcctg actggctgat atgccagcat tcctcgcgga cgtctccgcc tctccggcat
      241 tcttcttcgc ctcctcagcg tgacgcgccg cttcttccac catcagttca aaacgacgca
      301 gtgcctccgg ccggacgtca tcctccgaca tggcaccgag aaaatcattc agcgtccccg
      361 gttgagaatc ttcatacacg gtgatggtcc cggcatgtga cggcgggaat ccttccacca
      421 acagaataac gctgtactga ccgtactcaa cgtccatgct gtaacgcccg gcttcatccg
      481 gattttctga ggccagggtg ttcaccacca ccgtggtgct gttacgtttt gctttcagct
      541 ggattgtgca gttctgtacc ggttttcctg tgccgtcttt cagtacacct gaaatcttta
      601 ctgccatatt caccccacaa aaaagcccgc ctgaaccggc gggctgtcat aacactgtgt
      661 tacctggcta atcagaattt ataaccgaca cccacgatga aaccgtcagt gcgccagtcg
      721 ccactgccgg agcattcata agcaatatca atggccacgg attcggtcgg gttaacctgc
      781 acgccagctc cccacgccag agacgtgttg ctgtggcgac cgtcatcact tccggtcagc
      841 acgtcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga gaaagtcgac
      901 acacggctgt aagccatacc cgccatcgca tacgcgctga accattcatt cacgcgcaca
      961 gacggccccg ccatcacgct gaaccagcgg ttacg
//
LOCUS       DAZGCS010000374          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGCS010000374 DAZGCS010000000
VERSION     DAZGCS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.382"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..510)
                     /locus_tag="ACHK01_005452"
     CDS             complement(<1..510)
                     /locus_tag="ACHK01_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8100165.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
     gene            487..629
                     /locus_tag="ACHK01_005453"
                     /pseudo
     CDS             487..629
                     /locus_tag="ACHK01_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(824..937)
                     /locus_tag="ACHK01_005454"
     CDS             complement(824..937)
                     /locus_tag="ACHK01_005454"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO8100166.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          248 a          289 c          239 g          219 t
ORIGIN      
        1 ttttttggcg atatgcagcc cttgccccac ggttccgtac tgcccctttg acaggtccgc
       61 tttcggatgg ttaaggcggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
      121 gatgtcgtta tatttacagg cgacaccgcc cggtgtcacc gtactgcgac gcgccagctg
      181 cttaatgcgc gggtccggac ggtcatatgt ctccggcagc ggaaggcctt caccatatgc
      241 catgctgttt gactgccccg ccagaaccac aacaaagtaa tactccgggt ctctggtggc
      301 gctgattact gtgccttctc catccgacgg cttcaccaac acaggtgtgg tgacatcacc
      361 ttccgccgca atggcctgca tcagagtata aggcgtgatg gccaccgggc tgccaaatgg
      421 ctgccacccc tccttcagtt tttgtgtcag cttttccgca aggtctgacg gcgacgccgc
      481 cctgacaaca tcataatgtt taatcgacat cgaatttctc ccgtgtacag gaacagagtt
      541 aaaaagccgg aaccggaatc aaattacagg atggccatct gccagtggct ggtcgtaaaa
      601 aaaggccacg ccatgcgcag ccggaaataa agggataacg atgatagttt gagaaaaaca
      661 gaaacaacac ttttgtggca aagcatggtg ccgggtgcct cccggtgaat tcagtatcag
      721 cacctgaatc cgcgattatc ccatatacct ggttgctgat cgcccctccg cacaggggga
      781 ttcaccatgc agaagtgttt ttaataaaca gcaaacaaaa aaatcaagca ttatgcaggc
      841 tgtttctttt tatcaccggc cacagcaata ccacaatgcc gcagaccagc accccatccg
      901 ccagcaccga catgattctg ctggtgaaat ccaccatcac caccagaaac agcaggagtg
      961 cagccacagc caggcgcagt tttaccgtca caggt
//
LOCUS       DAZGCS010000375          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGCS010000375 DAZGCS010000000
VERSION     DAZGCS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.383"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..432
                     /locus_tag="ACHK01_005455"
     CDS             166..432
                     /locus_tag="ACHK01_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100167.1"
                     /translation="MRRKESNVSSLPELTNFEVSYSLVTNEVYLSASFTDNMACIPNW
                     PLQEFPDQLICISRAKAVALIEELQKTINYMDAGIDRSSGSLLQ"
     gene            493..>987
                     /locus_tag="ACHK01_005456"
     CDS             493..>987
                     /locus_tag="ACHK01_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100168.1"
                     /translation="MSEFSNLLNSIPGWLSSSLTALVGTLIGGWFTLKGVTQQAKLSK
                     VETERESLELQLSVLKGVKGEVFTLINLYNKRMKTHIDNIKPGQMLILTFPVGDDNFT
                     FYEQNANVIAKLNDSARDSIINIYTYSRSLIQSFKGNNKLIEDYEKILIGMADNNKDK
                     TMYKR"
BASE COUNT          340 a          190 c          169 g          288 t
ORIGIN      
        1 ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc ataagttcag caacacaaag
       61 atcccccccc ctttttctat aacggttcat ttgagactcc cccttgactc cctcttcgaa
      121 attgaagacc atcacctgta cataattaca gtcatggata atattatgcg cagaaaagag
      181 tcaaacgtta gcagtttgcc ggaactcacc aattttgagg taagctattc tctggttacc
      241 aatgaagttt acttgtctgc atccttcact gataacatgg cctgtatacc aaactggccc
      301 ttacaagaat ttccggatca actaatatgc atttctcgag caaaagctgt tgccctaatc
      361 gaagagctcc agaagaccat caactatatg gatgcaggaa tagatcgaag ttcaggtagc
      421 cttctccagt agctgacgag ttttataata atttgatata tatatatcgt tcaatattag
      481 cgagtactta taatgagtga atttagtaat cttttaaata gcatacctgg atggttatcc
      541 tcctccctca cagctctagt tggtactctt attggcggat ggtttaccct aaaaggagtt
      601 acccaacaag ctaagctgtc taaagtagag acagaaagag aatcccttga attacaactt
      661 tcagtattaa aaggagttaa gggagaagtt ttcaccctaa tcaatttata taataaaagg
      721 atgaaaacac acattgataa tataaaaccg ggacaaatgt tgattctcac attccctgta
      781 ggggatgata atttcacatt ctatgagcaa aatgcgaatg tcattgcaaa actaaatgat
      841 tctgctagag actcaataat taacatatac acatattcac gctcattaat acaatcattt
      901 aaaggaaaca acaaactcat tgaggattat gaaaaaatcc ttattggcat ggcagacaat
      961 aacaaagata aaaccatgta taagaga
//
LOCUS       DAZGCS010000376          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGCS010000376 DAZGCS010000000
VERSION     DAZGCS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.384"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            194..>986
                     /gene="cif"
                     /locus_tag="ACHK01_005457"
     CDS             194..>986
                     /gene="cif"
                     /locus_tag="ACHK01_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO8100169.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFE"
BASE COUNT          297 a          193 c          225 g          271 t
ORIGIN      
        1 atggatggag ttggtaaaaa gtggtgtggc tggcaatcca ggccgcgtca cagaaatgga
       61 caatgccgct gagagactgg cgaatggcat acaggcacca gaaaaatttc agacatgaca
      121 taatgcccat gccagaagtt ccttttgcac ctcgcctgta tttatagata aacatagagt
      181 gaatgaatga gatatgaaag acattaccct tccccccccg acgtccgcgt cctgtctgac
      241 aggggccata tctgtaaata ctgaagctgt attatctccc atgcaacaca cttcagcctt
      301 acatgtaaga gattttgctt ccctgtgctc acagaacctc aaagctaatg tattgctaaa
      361 tagtgatgac cacgaagtac ctatacatca gaaaaatcct gctgcaataa tgcaaaatat
      421 cgactctaac atcaaacaga tggcaacaga ctgggggatg tcgattgagg aggttgaggt
      481 tattataggg cgagagaaag gcattgtgga accctcctgc ggagttaccg ctaatgctat
      541 tatgaaacta tttctggaca aggatggctt cagttactgc tttgaaaatg aacagacact
      601 atcgctcgag cagcttcagg aacgcctgtc ctgtatgcct gaatgtaaga gctttgtatt
      661 acgtgttaat gatggtgcgc ttggtcatgc ttacattgtc gatattccca aaggagaaaa
      721 ctcttgtcgt cctgcattct tgtatcagtc agatttagga gagggcgtca ccagaaagtt
      781 aagatttgag gactggatga cgcataaagc attgactccg attttgctgg atgatatttg
      841 taattacttc tcctgcatgt ctcaaaataa gacagatttg gagcagattg caacgttatt
      901 tgatattgat gggaatgtta aaatgttacg aaaggaaaat attcaatatc aaaagcatga
      961 caattttagt ttccagttgt ttgagt
//
LOCUS       DAZGCS010000377          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGCS010000377 DAZGCS010000000
VERSION     DAZGCS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.385"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>986
                     /locus_tag="ACHK01_005458"
     CDS             <1..>986
                     /locus_tag="ACHK01_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8100170.1"
                     /translation="RNGGWVTEKDITIKGKTTSQYLASVVVDNLPPRPFNIRMRRMTP
                     DSTTDQLQNKTLWSSYTEIIDVKQGYPNTALVGVKVDSEQFGSQQVSRNYHLRGRILQ
                     VPSNYNPQTRQYSGIWDGTLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALY
                     VIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFV
                     QDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVE
                     DTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGL"
BASE COUNT          228 a          253 c          315 g          190 t
ORIGIN      
        1 cgtaatggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacctcgcag
       61 tatctggcct cggtggtggt ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc
      121 agaatgacgc cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcatac
      181 accgaaatca tcgatgtgaa acagggctac ccgaacacgg cactggtcgg cgtaaaggtg
      241 gattcggagc agttcggcag ccagcaggtg agccgtaatt atcatcttcg cgggcgtatt
      301 ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat ctgggacgga
      361 acgttaaaac cggcatacag caacaacccg gcctggtgtc tgtgggatat gctgacccac
      421 ccgcgctacg gcatggggaa acgtcttggt gcggcggatg tggataaatg ggcgctgtat
      481 gtcatcggcc agtactgcga ccagtcggtg ccggacggct ttggcggcac ggagccgcgc
      541 attacctgta atgcgtacct gaccacacag cgtaaggcgt gggatgtgct cagtgatttc
      601 tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac
      661 cgaccatcag ataaggtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggt
      721 gcgccgttcc gctacagctt cagcgccctg aaggaccgcc ataatgccgt tgaggtgaac
      781 tggattgacc cgaataacgg ctgggagacg gcgacagagc ttgttgaaga tacgcaggcc
      841 attgcccgtt acggtcgtaa tgtcacgaag atggatgcct ttggttgtac cagtcggggg
      901 caggcacacc gagccgggct gtggctgatt aaaacggaac tgctggaaac gcagaccgtg
      961 gatttcagcg tgggcgcaga agggct
//
LOCUS       DAZGCS010000378          971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGCS010000378 DAZGCS010000000
VERSION     DAZGCS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.386"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..279)
                     /gene="yqeK"
                     /locus_tag="ACHK01_005459"
                     /pseudo
     CDS             complement(1..279)
                     /gene="yqeK"
                     /locus_tag="ACHK01_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            473..963
                     /locus_tag="ACHK01_005460"
                     /pseudo
     CDS             473..963
                     /locus_tag="ACHK01_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          173 c          138 g          307 t
ORIGIN      
        1 acgtttcttt aaaagaatta atttcatcat ctgttaaact aaactcatca ttgacagatc
       61 gtgagatata actgttttta actttactct ttacgttgac tttattgaca gaattaacat
      121 tcacatatct tgaatttaat gtccatgatg ttgtttgcgt gagaaccttc tcagcattaa
      181 tgaatttttt aacggaatcc ttggtttctt tttcgagtcc gaatttacaa tatcatgcat
      241 atagaccatt tcatgaatct gcgaaaactc aatgtccatt gtatacctca catttttacc
      301 gtgactcgat gttactgttc aataatcacc ttccatcaat actaaaatta atacccctaa
      361 tgtgccgata acaaatatag tcattctacg taacgtctcc ataaggtgat atttgacatt
      421 atcagaagct gcgaattcgg attttgctct aatcaaatga gagaaatgta aaatgaagcc
      481 acgaaatatt aataatagcc tatcactgca accattagtt cctgatcagg agaacaaaaa
      541 taagaaaaat gaagagaaat ccgttaatcc agttaaaatc acaatggggt ctggtttaaa
      601 ttatattgaa caagaatctc ttggaggaaa atatctaata catgatttgt caataaagat
      661 agcggatatt tctgaagaga taattcagca agcaatatta tctgctatga gcatatataa
      721 attttcgata acagatgatt taatgagtat ggctgtaaat gaactcataa aactgaccaa
      781 aatagagaat aatgtagacc tgaataaatt cactactata tgcacagacg ttctatcccc
      841 cctcgtcacc agacataata aagaaaaaaa acaaacgaca ttctaccctt cgcaaaaatc
      901 ccctttttaa ttttcattga aaaaatggca attgatacta tgaatccagg tcatacacat
      961 taaggcattt a
//
LOCUS       DAZGCS010000379          961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGCS010000379 DAZGCS010000000
VERSION     DAZGCS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.387"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..678
                     /locus_tag="ACHK01_005461"
     CDS             37..678
                     /locus_tag="ACHK01_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO8100171.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            709..831
                     /locus_tag="ACHK01_005462"
                     /pseudo
     CDS             709..831
                     /locus_tag="ACHK01_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
BASE COUNT          307 a          148 c          223 g          283 t
ORIGIN      
        1 aaagcaaaat taagttatta aaatggaggg agaggaatgc ctgtagattt aacgccttat
       61 attttacctg gggttagttt tttgtctgac attcctcaag aaaccttgtc tgagatacgt
      121 aatcagacta ttcgtggaga agctcaaata agactgggtg agttgatggt gtcaatacga
      181 cctatgcagg taaatggata ttttatggga agtcttaacc aggatggttt atcgaatgat
      241 aatatccaga ttggccttca atatatagaa catattgaac gtacacttaa tcatggtagt
      301 ttgacaagcc gtgaagttac agtactgcgt gaaattgaga tgctcgaaaa tatggatttg
      361 ctttctaact accagttaga ggagttgtta gataaaattg aagtatgtgc atttaatgtg
      421 gagcatgcac aattgcaagt gccagagagc ttacgaacat gccctgttac attatgtgaa
      481 ccagaagatg gggtattcat gaggaattca atgaattcaa atgtttgtat gttgtatgat
      541 aaaatggcat taatacatct tgttaaaaca agggcggctc atcctttgag cagggaatca
      601 atcgcagttt caatgattgt aggaagagat aattgtgctt ttgaccctga cagaggtaac
      661 ttcgttttaa aaaattaaga tagcaatggc gggttaactt ttcgtaaaat gaaattaatt
      721 ctcggtacgg catgtaaaac gcggcacctc gtatgcaaga acgtactgcg gttggttggt
      781 gaactttcga tagtgctagt attggatgat ttccagccgt taccgatttt atataacttc
      841 agtggaatta ttcaacacac aggcaaaacg gaataagctg agaaaaaaag cgtgctttcc
      901 caatctgggg taaatcacgt ctgaatgaca cctgaaaaca ggtggtggcc tcaagaaggc
      961 t
//
LOCUS       DAZGCS010000380          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGCS010000380 DAZGCS010000000
VERSION     DAZGCS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.388"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHK01_005463"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHK01_005463"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO8100172.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          254 a          237 c          211 g          252 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ttcatgagtt
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgc
      601 cgaccaatga cagccagcaa atcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgccg caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcg
//
LOCUS       DAZGCS010000381          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGCS010000381 DAZGCS010000000
VERSION     DAZGCS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.389"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..161)
                     /locus_tag="ACHK01_005464"
     CDS             complement(<1..161)
                     /locus_tag="ACHK01_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100173.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..555)
                     /locus_tag="ACHK01_005465"
     CDS             complement(142..555)
                     /locus_tag="ACHK01_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8100174.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(582..>943)
                     /locus_tag="ACHK01_005466"
     CDS             complement(582..>943)
                     /locus_tag="ACHK01_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8100175.1"
                     /translation="ALQRFDYIKFVSDAEQQETTKHDVVHINQRYLETASLLVAMSLW
                     HSHSLKGTLASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEQTG
                     KAEATEPVTSKKHSKAS"
BASE COUNT          210 a          293 c          222 g          218 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccggctc agtcgcttct gctttcccgg tttgttcagg atcggcatcg
      661 tgccggttat ccagcatacc tgaaagataa agcacccggt tcgttgcctg attcagtgca
      721 tcagcaggcc atcccagcat cacttcacgg cggatctgct gcatctctgt ctccggagag
      781 gccagagtgc ctttgaggga atgggaatgc catagcgaca tcgccacaag cagggatgcc
      841 gtttccagat atcgctggtt aatgtgcacg acatcatgct tcgttgtctc ctgttgttct
      901 gcgtctgaaa caaactttat ataatcaaac cgctgcagcg cag
//
LOCUS       DAZGCS010000382          934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGCS010000382 DAZGCS010000000
VERSION     DAZGCS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.390"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..194)
                     /locus_tag="ACHK01_005467"
     CDS             complement(<1..194)
                     /locus_tag="ACHK01_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100176.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHK01_005468"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHK01_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(573..>934)
                     /locus_tag="ACHK01_005469"
     CDS             complement(573..>934)
                     /locus_tag="ACHK01_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8100177.1"
                     /translation="ALQRFDYMKFVSDAAQQETTKHNAVHINQRYLETASLLVAMSLW
                     HSHSLKGTLASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEPTG
                     KAEATEPVTSKKHSKVS"
BASE COUNT          205 a          286 c          220 g          223 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacgt
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctca ccttcgaatg cttttttgat
      601 gttaccggct ctgtcgcttc tgctttcccg gttggttcag gatcggcatc gtgccggtta
      661 tccagcatac ctgaaagata aagcacccgg ttcgttgcct gattcagtgc atcagcaggc
      721 catcccagca tcacttcacg gcggatctgc tgcatctctg tctccggaga ggccagagtg
      781 cctttgaggg aatgggaatg ccatagcgac atcgccacaa gcagggatgc cgtttccaga
      841 tatcgctggt taatgtgcac ggcattatgc ttcgttgtct cctgttgtgc tgcgtctgaa
      901 acaaacttca tataatcaaa ccgctgcagc gcag
//
LOCUS       DAZGCS010000383          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGCS010000383 DAZGCS010000000
VERSION     DAZGCS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.391"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHK01_005470"
     CDS             <1..75
                     /locus_tag="ACHK01_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100178.1"
                     /translation="EGIRITDIDTSGIFDSDDMAIKAA"
     gene            148..288
                     /locus_tag="ACHK01_005471"
     CDS             148..288
                     /locus_tag="ACHK01_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO8100179.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            281..424
                     /gene="kil"
                     /locus_tag="ACHK01_005472"
     CDS             281..424
                     /gene="kil"
                     /locus_tag="ACHK01_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO8100180.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIH"
     gene            500..796
                     /locus_tag="ACHK01_005473"
     CDS             500..796
                     /locus_tag="ACHK01_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO8100181.1"
                     /translation="MNAYYIQDRLEAQSWARHYQLIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            802..>911
                     /locus_tag="ACHK01_005474"
     CDS             802..>911
                     /locus_tag="ACHK01_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO8100182.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          263 a          213 c          210 g          225 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatggct
       61 atcaaggccg cctgagtgcg gctttaccgc ataccaataa cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgagcga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gttccaaaaa atggataaaa cacttatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat acagaaactg agatcaagca aaagcattca
      421 ctaaccccct ttcctgtttt cctaatcagc ctggcatttc gcgggcgata ttttcacagc
      481 cattttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag
      541 ctgggcacgt cactaccagc tgatcgcccg tgaagagaaa gaggcagaac tggcagacga
      601 catggaaaaa ggtcttccac aacatctgtt tgaatcactc tgcatcgatc atttgcaacg
      661 ctgcggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga
      721 gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat
      781 tgattcagag gtataaaacg gatgagtaca gcactcgcaa cgctggcagg gaagctggct
      841 gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg
      901 gcatttaaag g
//
LOCUS       DAZGCS010000384          901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGCS010000384 DAZGCS010000000
VERSION     DAZGCS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.392"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHK01_005475"
     CDS             <1..75
                     /locus_tag="ACHK01_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100183.1"
                     /translation="EGIRITDIDTSGIFDSDDMTIKAA"
     gene            148..312
                     /locus_tag="ACHK01_005476"
     CDS             148..312
                     /locus_tag="ACHK01_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO8100184.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            281..424
                     /gene="kil"
                     /locus_tag="ACHK01_005477"
     CDS             281..424
                     /gene="kil"
                     /locus_tag="ACHK01_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO8100185.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(429..539)
                     /locus_tag="ACHK01_005478"
                     /pseudo
     CDS             complement(429..539)
                     /locus_tag="ACHK01_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
     gene            complement(536..>901)
                     /locus_tag="ACHK01_005479"
     CDS             complement(536..>901)
                     /locus_tag="ACHK01_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO8100186.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
BASE COUNT          234 a          221 c          208 g          238 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatgact
       61 atcaaggccg cctgagcgcg gcgttaccgc ataccaatta cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgaacga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gtcccaaaaa atggatcaaa caattatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat actgaaactg agatcaagca aaagcattca
      421 ctaacctgaa cttctgaagc tgtgatgacg ccgagccgta atttgtgcca cgcatcatcc
      481 ccctgttcga cagctctcac gtcgatcccg gtacgctgca ggataatgtc cggtgtcatg
      541 ctgccacctt ctgctcagtg gctttctgtt tcaggaatcc aagagctttc actgcttcgg
      601 cctgtgtcag ttctgacgat gcgcgaatgt cgcggcgaaa tatctgggaa cagagcggca
      661 ataagtcgtc atcccatgtt ttatccaggg cgattagcag agtgttaatc tcctgcatgg
      721 tttcatcgtt aaccggagtg atgtcgcgtt ccggctggcg ttctgcagtg tatgcagtat
      781 tttcgacaat gcgctcggct tcatccttgt catagatacc agcaaatccg aaggccagac
      841 gggcacactg aatcatagct ttatgccgta acatccgttt aggatgcgac tgccacggcc
      901 c
//
LOCUS       DAZGCS010000385          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGCS010000385 DAZGCS010000000
VERSION     DAZGCS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.393"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHK01_005480"
     CDS             <1..375
                     /locus_tag="ACHK01_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8100187.1"
                     /translation="LFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGFYRVPL
                     SSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRH
                     RHWHASAFTGICNDLAAASACT"
     gene            372..>899
                     /locus_tag="ACHK01_005481"
     CDS             372..>899
                     /locus_tag="ACHK01_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8100188.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDIS"
BASE COUNT          182 a          245 c          286 g          186 t
ORIGIN      
        1 ctgttccggg atgcatacca tctggcggga attgatatgc cggattttga gcgtgaggat
       61 gactggtggc gcaacggtca gaacctttac ctggacaata tggcggtcac cggcttttac
      121 cgggtgcccc tgtcctctgc acaggcgggc gatatcctgc tgtgctgctt tggcgcatcg
      181 gtggccaatc atgccgccat atactgcggc aacggtgagc tgcttcacca tctgcctgaa
      241 caactgagta aacgggagag gtattccgaa aaatggcaac gacgaacgca ttcagcctgg
      301 cgtcaccgcc actggcacgc atctgccttc acggggattt gcaacgattt ggccgccgcc
      361 tcagcctgta cgtgaacacg gcagcggaag ccatccgggc gctgtcgtta caggtgccgg
      421 gattccgccg tcagatgaac gaaggctggt accagatacg tattcgcggt gaggacacgg
      481 caccggaggc ggtgtacgcc cgtcttcacg aacctctggg tgagggggcg gtcatccata
      541 ttgtgccgcg actggccgga gccggaaagg gcggactgca gattgtgctg ggggcggcag
      601 ccatcgtggg ctctttcttc acggccggag catcgatggc agcctggggt gcagcgctga
      661 gtgccggcgg tttttctgcc accacgatgc tgttttcact gggtgccagc atgatactgg
      721 gtggtgtggc acagatgctg gccccgaagc caaaaacacc ggaatacagg gcaacggata
      781 acggtaaaca gaacacgtac ttttcgtcgc tggataacat gattgctcag gggaacccga
      841 tgccggtgcc ttacggtgaa atgctggttg gctcacggcg aatctcccag gacatcagt
//
LOCUS       DAZGCS010000386          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGCS010000386 DAZGCS010000000
VERSION     DAZGCS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.394"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..548)
                     /locus_tag="ACHK01_005482"
     CDS             complement(<1..548)
                     /locus_tag="ACHK01_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8100189.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGA"
     gene            525..668
                     /locus_tag="ACHK01_005483"
                     /pseudo
     CDS             525..668
                     /locus_tag="ACHK01_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            744..854
                     /locus_tag="ACHK01_005484"
                     /pseudo
     CDS             744..854
                     /locus_tag="ACHK01_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          229 a          246 c          218 g          202 t
ORIGIN      
        1 gcgccctggg tgaatgccga accaccacga cagcatggta ccagcaggat ccccgcgtta
       61 ttcgggatat acgggagcag ttttttggca atatgtaacc cctggccgac acagccgtac
      121 tgccctttgc tcaggtctgc cttcggatga ttcagcgtac tcatatcctg cacatcatgc
      181 aggcagtggt cggccggaat aatatcgtta tatctgcatg cagccccacc cggcgtaact
      241 gtactgcggc gcgccagctg tttaatgcgc ggatccggag catcgtatga atccggcagc
      301 ggaagccctt caccgtaagc catggcattg gactgcccgg ccagtacgat gacgtagtac
      361 caatccggct cagttgcacc actgaccacc acatcacctt ctgctgtaat cgcctgcatc
      421 agggtataag gggttatggc caccggacta ccaaacggct gccagccctc tttcagtttg
      481 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc ataatgttta
      541 atcgacatcg aatttctccc gtgtacagga acagagttaa aaagccggaa ccggaatcaa
      601 attacaggat ggccatctgc cagtggctgg tcgtaaaaaa aaggccacgc catgcgcagc
      661 cggaaataaa gggataacga tgatagtttg agaaaaacag aaacaacact tttgcggcaa
      721 agcatggtgc cgggtgcctc ccggtgaatt cagtatcagc acctgaatcc gcgattatcc
      781 catatacctg gttgctgatc gcccctccgc acagggggat tcaccatgca gtagtatttt
      841 taataaacag caaataaaaa aatcaagcat tatgcaggct gtttcttttt atcac
//
LOCUS       DAZGCS010000387          892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGCS010000387 DAZGCS010000000
VERSION     DAZGCS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.395"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>892
                     /locus_tag="ACHK01_005485"
     CDS             <1..>892
                     /locus_tag="ACHK01_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO8100190.1"
                     /translation="KAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPLS
                     LLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAVL
                     AALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLA
                     RLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNF
                     LSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWT
                     SDAWAWIGDHTLGALSLITDV"
BASE COUNT          200 a          219 c          295 g          178 t
ORIGIN      
        1 ataaggctgc catgcgcaca ctgcccgcac agttcacgga tattgtcact cagcttgccg
       61 gtggtcagaa tcccttcctt atcatgctgc agcagggggg gcagatcagc gattcattcg
      121 gtggaccgct cagcctgctt accctgctga aggaggaact tctcgggatc agggatgcct
      181 ctgaatcatc agaggagtcg ctgtcagata cggcaaatgc actggctgaa aatgcccgga
      241 atgccggtga gctgggacga tttatgtcgg tggcccgtgt ggcggcaggt ggcggggttg
      301 ccgtactggc cgcgcttgct gccgccgcct ggcaggcaga gcaggctgac cgggccttat
      361 tgcgttcact gatcctgacc ggaggggcgg ctgccaccac aacggcagaa ttgtggaaaa
      421 tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca gaaaatctgg
      481 cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg ggggaaacct
      541 ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa gcctttgaag
      601 ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat aacttcctga
      661 gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa caggctgcgg
      721 tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag caacttgata
      781 aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact tctgacgcat
      841 gggcatggat aggtgatcat acactggggg cactcagtct gatcactgac gt
//
LOCUS       DAZGCS010000388          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGCS010000388 DAZGCS010000000
VERSION     DAZGCS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.396"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..833)
                     /locus_tag="ACHK01_005486"
     CDS             complement(<1..833)
                     /locus_tag="ACHK01_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO8100191.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VT"
BASE COUNT          192 a          289 c          204 g          192 t
ORIGIN      
        1 gtcaccccgc tggcctcatt cgcaccgccc acccaggcca taaaggcgtt ttccaccttc
       61 gtgatactac tggaaaccgt ttccggcatg gccgcatatt catcacgtaa tatccccagc
      121 tggctgatta acgcggggac cactttatcc gctgtcagtt ttccgtcatc cgccattgcc
      181 ttcagatctt tacgggccac gcccataccc gcagccagtg cacgtacgat ccggtcacca
      241 ctttcattga ccgaattaaa ctcctcacca cgcaatacac cctgcgccag cgcctgactg
      301 aactgggtga tcaccgagcc cgcctctgcc gtactggcac cggagatttt cagccctgtc
      361 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcattgag
      421 gcggctgagc gggcaaacag ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg
      481 atatccatca gcactttctg tgatgacgaa aattcatcag atgactgcga cgcctgtttc
      541 agacgggcat tcacggaact ccactcatcc gccagtgaaa tcaggtgtcc ggtggcaaag
      601 gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
      661 tgcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg acgtccgcca
      721 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
      781 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
      841 tctccgggca aaaaaaacct gccacagcag gttttca
//
LOCUS       DAZGCS010000389          870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGCS010000389 DAZGCS010000000
VERSION     DAZGCS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.397"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..131
                     /gene="xisR"
                     /locus_tag="ACHK01_005487"
     CDS             <1..131
                     /gene="xisR"
                     /locus_tag="ACHK01_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8100192.1"
                     /translation="REYKHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            151..>870
                     /locus_tag="ACHK01_005488"
     CDS             151..>870
                     /locus_tag="ACHK01_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO8100193.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLANNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELC"
BASE COUNT          279 a          180 c          199 g          212 t
ORIGIN      
        1 gaagggaata taagcatgtc gctcatgacg gtatgccatg ggataacagt ccatgctttt
       61 acaacctgga agaaattgat cgctggattg agcgccaggc atctgcaaga ccaagacgtc
      121 atcttgcttg actaaaagca acacgaacca atgagagaag ctgaaatgaa atatccgaca
      181 ggcgtggaaa accatggagg gaaattacgt atctggtttg tttataaagg tgtaagagtc
      241 cgggaaaatc ttggggttcc tgacacagca aaaaacaggc gcattgcagg tgagctacgc
      301 gcctctgttt gttacgcaat aaaaactggc gctttcgact atgcaaaaca gtttcccgcc
      361 tcacacaatc tggaaaaatt tggtgaggcc cgacaagatt taaccataaa agaactggct
      421 gaaaaatttc tggcactgaa agaaactgaa gtcgcaaaaa cgtcactcaa cacgtaccgt
      481 gccgtcatca aaaatattct gagcataatc ggtgaaaaaa atcttgcatc atcgattaat
      541 aaagaaaaat tgctggcggt acgtaaagag ttactgactg gataccagat ccccaaaagt
      601 aactatattg ttacacaacc agggagatcg gctgttaccg taaataatta catgacaaat
      661 ctttacgccg tgttccagtt tggtgttgat aacggttatc tggcaaacaa tccatttaag
      721 gggatctcac cattaaagga gtcgagaacc atcccggatc cactttcgcg ggaggagttt
      781 atccgcctta ttgacgcgtg cagaaatcag caagccaaaa atttatggtg tgtttccgtt
      841 tatactggga ttcgccctgg tgaactgtgt
//
LOCUS       DAZGCS010000390          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGCS010000390 DAZGCS010000000
VERSION     DAZGCS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.398"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..420
                     /locus_tag="ACHK01_005489"
     CDS             <1..420
                     /locus_tag="ACHK01_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO8100194.1"
                     /translation="KVQFPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSY
                     VVPLAFVKNPEKTLTVNTGALLTMSVSVNGGTPPYKYAWKKDGQPVEGQTTDTFSKAN
                     AQSGDKGAYTCMVMDSAEQPQSITSDACTVTVNGAGG"
     gene            426..800
                     /locus_tag="ACHK01_005490"
     CDS             426..800
                     /locus_tag="ACHK01_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8100195.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
BASE COUNT          191 a          205 c          282 g          189 t
ORIGIN      
        1 aaggtgcagt ttccgtccgg taagggcttt aagttcctgg cggaagtgcg tcagcacacc
       61 tggtcatccg gtaccaacgg cgtggtggct gcaacgtttt cacttcgcct gaagggtaaa
      121 ccggtgtcct atgtggtacc gctggcgttt gtgaaaaatc cggagaagac acttaccgtg
      181 aataccggtg cgctgctgac aatgtcagtc agtgtcaacg ggggaacgcc gccttataaa
      241 tacgcctgga agaaggatgg tcagccggtc gagggacaga ctactgacac tttcagtaaa
      301 gccaatgcgc agtcaggtga taagggggct tatacctgca tggtaatgga ttctgcagaa
      361 cagccgcaga gcattacctc tgatgcgtgt acggtaacgg ttaatggtgc gggcggataa
      421 ggcttatggc aaaagatctg aaaacactgg cgctggccag actgtcgggg ttccgtcata
      481 aaacggtgaa ggtgccggaa tggagaaatg tcagcgtggt gctgcgggag ccttcggcag
      541 aggcctggta tctgtggcag gaagtgctca atggtgatgg agaggatgac gataccctgt
      601 cggtggtggc gaaaacccgc cgtaacctgg aagcggatgt gacgctgttc tgcgatgtcc
      661 tgtgtgatac ggatctgcag cgggtgttcg ctccggacga ccgtgagcag gtgctggccg
      721 tctatggtcc ggtacatgcc cggttgctgc gtcaggcact ggaactgatc gctgatgcag
      781 agtcggccag aaaaaagtag cccgcccgga aattcgcttt ctgatgcgac ttgcgctccg
      841 tctggggcgc accttatccg aactgcg
//
LOCUS       DAZGCS010000391          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGCS010000391 DAZGCS010000000
VERSION     DAZGCS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.399"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..864
                     /locus_tag="ACHK01_005491"
     CDS             <1..864
                     /locus_tag="ACHK01_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO8100196.1"
                     /translation="LLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTLTCSGYEGRF
                     WISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDDVQFTMLNKD
                     VYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTIFFDGYIAPGWTDYPV
                     TELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFITDVQAADLLCAISNEA
                     VMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVKINE
                     DFYFTVSLVCD"
BASE COUNT          258 a          133 c          192 g          281 t
ORIGIN      
        1 ttgttgtcag tcataggaac cggtcgcctc tatattacgc ctgaagggca tgcttgccat
       61 gcatattttc gcgaagtcag cggtaatggt atccgcttca cgttaacatg ctcaggctat
      121 gaggggcgtt tttggatatc agaagaacag ttcattcagt ggtgccagga gttattcccg
      181 tatagcgaaa gccggctgat accggaagat atgataaaac tcatgatctt atgggtaatg
      241 cagactgctt taccagaagg tgatgtgtct gttgatgatg ttcaatttac gatgctaaat
      301 aaagatgttt atccggtaat agaaaataat aatggagaaa acagacttaa tgtaattata
      361 ctcgaaataa cggtacagag tttacaatat ctaattaatg aaaattggca atttgttcca
      421 cattctaata ccattttctt tgatgggtat attgctcctg ggtggactga ttacccggtt
      481 acagagctga ctgttggtga tagtttaagg ctctatcatg ttgatgacag caaagaaaga
      541 gagtgttggc ttgttattaa taacccttta gcaaccgtaa aactttgtga taataattta
      601 tttattacgg atgtccaggc tgcagatttg ttatgtgcta tcagcaatga ggctgtaatg
      661 gatcgcattt attgtgtgat tggaacaatt catgttgata tacatatgtt gcgcaatgcc
      721 aaaaaagatg atattatcga ttcaactgga tatcatcttt ttggtgggtg ccggttaata
      781 cgcaataata cgactatagc atacgggagc attgtaaaaa tcaacgaaga tttttatttt
      841 actgttagtt tagtatgtga ttaa
//
LOCUS       DAZGCS010000392          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGCS010000392 DAZGCS010000000
VERSION     DAZGCS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.400"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            576..>854
                     /locus_tag="ACHK01_005492"
     CDS             576..>854
                     /locus_tag="ACHK01_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFO8100197.1"
                     /translation="MNPITLQIISNAIVLLGVLVAIGTIIYNVRTAKKTQTANFLFES
                     RQDMQYIESLHTLKQVHRSGKSFRSYVFPCDGCIITDEEMAERRKFQYI"
BASE COUNT          226 a          195 c          182 g          251 t
ORIGIN      
        1 cagataattt cacgacgcct tccagtttat cgccaccatc gacggtcaga ctcttacgca
       61 attccaccgc gttgactgca tgttctcgct gcctcagtgc gattttgacg gccttacctt
      121 tcaccccatc aaatcaaaag ccctcacgcc tttttcatgc tcagcgtgag aaatatggcc
      181 attctgttgt gtctctgcga catcgttcag ggaatgctat ttaccccctg aaacgcgggc
      241 tgttcccccg tcacctgcgc gcagaaaaag cgcgtttttt tgtgcacgca cggatccttg
      301 acggatccag ccgccatgcg ggccggaagg gtaaaaagtc gttcaaaaaa attgtgcaaa
      361 tttgtgcact tttgtgcagg taagcatgcc tatatatttg accgtggctt gcctttgatg
      421 tagtttatac gtcacaaaaa ccacaaaacg tggttatgat ttctatgagg gggttgatat
      481 gaaaaaaatg tatgatgaat ttgatggttt ctaagctacg ttgacctcta atgaggcggg
      541 gatttgctcc ccgccttttt tatggatgaa ataggatgaa cccaataaca ctacaaatca
      601 tcagcaatgc tattgttctg cttggcgttt tggttgccat tggaaccatt atctacaatg
      661 tgcgtaccgc aaaaaaaact caaacggcca actttctttt tgagagtcgg caagatatgc
      721 aatatataga gtcattgcat accctgaaac aagtgcatcg ttcaggcaaa tcatttcgtt
      781 cttatgtttt tccttgtgat ggttgcataa taacagatga agaaatggcc gagcgtcgaa
      841 aatttcaata tatt
//
LOCUS       DAZGCS010000393          847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGCS010000393 DAZGCS010000000
VERSION     DAZGCS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.401"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..89
                     /locus_tag="ACHK01_005493"
                     /pseudo
     CDS             1..89
                     /locus_tag="ACHK01_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            89..250
                     /locus_tag="ACHK01_005494"
                     /pseudo
     CDS             89..250
                     /locus_tag="ACHK01_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(300..653)
                     /gene="cmi"
                     /locus_tag="ACHK01_005495"
     CDS             complement(300..653)
                     /gene="cmi"
                     /locus_tag="ACHK01_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO8100198.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYNCEEGYVEYAND"
BASE COUNT          266 a          165 c          142 g          274 t
ORIGIN      
        1 taaaaagagt taatggaatg gatagtatta aattacttga taaagttatt atctatcaac
       61 aacagaatga gcaattatat ccagcacagg cgttgttcgt tcatatgatg ataaatacga
      121 ttttaacgcc agcactcacc gtggcatcat cggagagtcg ctcacaaggc tcggggcgat
      181 gttttctggt aaagagtacc agatactgct tcctggtgaa attcacatta aagaaagtgg
      241 taagcgataa tataagggcc gggattccgg cctttttatg cagcctttat aacagtgtat
      301 tagtcattcg catattcaac atatccctcc tcacaattgt atatgccata atacattctc
      361 ttaccctccg ggccttcagc tttaaatggg acaagaagtg ttttttcatc ttcaacatct
      421 gttaaacttc tctcaatctt ttcccatgta attttcggga tttctgtacc aagaagttga
      481 acatcgttgt cccagcgttt cattctttgc aggtaatcat tttttacaaa cgttctggcc
      541 tgttcatcgc tgacctgata gcatgccgga cctttatctt cagaccagaa aaaaacagca
      601 gcaagagcaa taatcgttaa aataaaaata aatttcatgc taattacttt cataaaatct
      661 ccacaacttc tgtcgttcat tcgatataaa aaacattacg aatatatccg tacaatgtga
      721 gcatactgtg aagtggcttt aacaattatg aaataataca atagatcaca tattgcagtg
      781 gtctcttttt tatagaagaa atcacaattc ataattgtga cttattatgc atgtttgttt
      841 ttcagca
//
LOCUS       DAZGCS010000394          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGCS010000394 DAZGCS010000000
VERSION     DAZGCS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.402"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>845
                     /gene="yfdF"
                     /locus_tag="ACHK01_005496"
     CDS             <1..>845
                     /gene="yfdF"
                     /locus_tag="ACHK01_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO8100199.1"
                     /translation="ISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGISTCIK
                     HLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPANYKDK
                     ELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESG
                     NSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQ
                     YCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYN
                     FQVDDA"
BASE COUNT          319 a          149 c          156 g          221 t
ORIGIN      
        1 atttcaatca acaataccag cgcaacttac ccagaatcca ttaatgaaaa taacaatgat
       61 gaagttaatg gattagtaca agagctcaaa aaccttttta atggtaagga aggaataagc
      121 acctgtatta aacatctact tgagcttata aaaaacgcca tacgagtaaa cgacgatcct
      181 tatagattta atattaataa ttcctcagtt acttatattg atattggctc caatgataca
      241 gaccatatta ctattggtat cgacaaccaa gaaccaatag aattacctgc gaactataaa
      301 gacaaagaac tcgtccgtac tatcattaat gacaacatag ttgagaagac tcatgatatc
      361 aataacaagg aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt
      421 atcttcgata agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg
      481 aatagcgaac caacggactt atttacctgg tacggtaaag ataaaaaagg cgactctctc
      541 gctattgtaa ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag
      601 gacgactacc acattcaaag aggaattcgt attaatggtg atagtctcac ccaatattgt
      661 agtgaaaaag ccaggaatgc ttcagcgtgg tttgaaagca gtaaagctat catggcagaa
      721 tcatttgcaa caggttccga tcatcaggtt gtaaacgagc tcaacgggga aagactgaga
      781 gaacctaacg aagtttttaa acgtttaggt cgagcaataa gatataattt tcaagtggac
      841 gatgc
//
LOCUS       DAZGCS010000395          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGCS010000395 DAZGCS010000000
VERSION     DAZGCS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.403"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..662
                     /locus_tag="ACHK01_005497"
     CDS             204..662
                     /locus_tag="ACHK01_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100200.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            762..843
                     /locus_tag="ACHK01_005498"
                     /pseudo
     CDS             762..843
                     /locus_tag="ACHK01_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          284 a          145 c          137 g          277 t
ORIGIN      
        1 aacacactct tttcattaaa tcaatcacct gattaaacca tgaaagtgtg atgtaagtca
       61 aaattactga tactatcaat tgataatgat tatctctatc attatcgtga aattcgagaa
      121 tgaaaggaga agtagtcatt ataagccttt tataaatcgt catcatcata gtaaagtaga
      181 acacttttta tatgagttac tatatgaaaa tatactggac aagaaaacgt attcctgaac
      241 tttcagcact tccaccttca ttaagaaaga aaaattttac tgacgcatat aatgcagcat
      301 cttctcatat cgaatactgg ataggtgctg gcgtctcatt tatctcgatg atgattttat
      361 ccagagtata tgactttctt ttacccgccc aagatacttt cccgggtgat atcatcagaa
      421 gtttatgtgt tgtgtgtcca tccatattaa tctggtttca gttttcggta tatgtcatga
      481 ggaaatatta cagacatatt ctggtgcgag gaaaagaaac cgaaaccatt tcagaaaggt
      541 tgatacgtga ggctgataca cgagaatatg aactatggcg tccggtccgt cgctttttct
      601 ccattgtttt cctcattgtt ctattaggtt gcattcattc actgataaca actataaaat
      661 aaaagttatg gctgttctga tacaggaaaa taaacgttca ttctatggtt acctcttcct
      721 gttatataga agaattttaa acgacaagta agggaatata aatgaaaata acaaaaaata
      781 aaaatgcaac tataaaaaac agtcatcagt cggctgcttt tcaatgttta ccttatcatt
      841 ttc
//
LOCUS       DAZGCS010000396          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGCS010000396 DAZGCS010000000
VERSION     DAZGCS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.404"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>836
                     /locus_tag="ACHK01_005499"
     CDS             <1..>836
                     /locus_tag="ACHK01_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO8100201.1"
                     /translation="NIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGW
                     NIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTD
                     KIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEF
                     INERKLSDKAYIGVYKTTGTGKYDWLTPESIGVNDVTPADESTWCIGKGRCVDDFLFK
                     DVSTLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFK
                     NL"
BASE COUNT          300 a          146 c          164 g          226 t
ORIGIN      
        1 aaaacattat caagaagata ttaacagaaa tatctaaaat gccagaagat agtattttta
       61 ctaagatcaa tacaaggatt cctcgagaca caatgcccat ccttcgtcgt tatcacctat
      121 ggcctgatgg atggaatatt cgtgggctca atggattcat gctatcacat aaaggtagtg
      181 aagtgattga tgctgttatc gcaggccaga atcaggctta cagggaacta agaagaataa
      241 gagataatat tcatagtgaa atatacttca aacaaactga tgaattgtcc tcacttccag
      301 atacagacaa aattggaggg attctggtaa aaaaatacct ttcaggaagt cttttttcaa
      361 aattcagaca agatactatt atcccggagg cattgagcac cctccagata tcaggtcctg
      421 acttgattca aagaaagatg ttgcaatttt tcaggagtag aggggtgtta ggtgaagagt
      481 tcattaatga aagaaaactg agtgataaag cttatattgg tgtctacaaa acaactggca
      541 cagggaaata tgactggtta acccctgaat cgatcggcgt taatgatgtc acgcccgcag
      601 atgaaagtac ctggtgtata ggaaaaggcc ggtgtgttga tgacttcctg ttcaaagatg
      661 tttcaacact aaaaacagaa aatcttccag aattattctt aacaaaaata gatactgata
      721 cgtttttctc tcagtggtca accaaaacca agaaagatct gcaaaaaaaa atacaagacc
      781 ttactgtacg ttataatgag ctaattgact cgtcgactat cgactttaaa aatcta
//
LOCUS       DAZGCS010000397          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGCS010000397 DAZGCS010000000
VERSION     DAZGCS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.405"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>833
                     /locus_tag="ACHK01_005500"
     rRNA            <1..>833
                     /locus_tag="ACHK01_005500"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          227 a          183 c          261 g          162 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg
      661 gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat
      721 agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag
      781 ctgcggcagc gacgctgatg cgttgttggg taggggagcg ttctgtaagc ctg
//
LOCUS       DAZGCS010000398          831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGCS010000398 DAZGCS010000000
VERSION     DAZGCS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.406"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            10..795
                     /locus_tag="ACHK01_005501"
     CDS             10..795
                     /locus_tag="ACHK01_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100202.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          267 a          174 c          183 g          207 t
ORIGIN      
        1 tgtccaatta tgaattcaaa aaagctttgt tgcatatgtg tgttattctc gctgcttgca
       61 ggatgtgcct ctgaatcttc tattgatgaa aagaagaaaa aagctcaagt cacacaaagt
      121 aatattaata aaaacactcc ccagcaactg acagacaaag atttattcgg taatgaaacc
      181 actctggccg tatccgaaga ggatattcaa gctgcgcttg atggagatga gttccgcgtt
      241 ccccttaatt ctcctgtaat acttgttcaa tccggcaacc gcgcaccgga aaccattatg
      301 caggaggaga tgcgaaaata ttatactgtt tccacattct ctggtatccc ggacaggcaa
      361 aagcctctga cttgtaacaa aaacaaagat aaaaatgaga acgaggatgt tgccagtgct
      421 gagaatatga actggatgca ggcactgcgt tttgtggctg caaaagggca tcagaaagcg
      481 attattgttt tccaggatac gttgcagaca ggaaaatatg actctgcgct gaaatcaaca
      541 gtatggtccg actataaaaa tgaaaaactc accgacgcta tctccctgcg ctacctggta
      601 cgtttcacgc tggtggatgt ggcaacaggt gagtgggcta cctggtcgcc ggtgaattat
      661 gaatataaag tgcttccacc attgcccgac aagaatgaag ccagtacgac tgatatgaca
      721 gagcagcaaa tcatgcaact taagcaaaag acctataaag cgatggtaaa agatttggtt
      781 aaccgctatc aataataaat tatatctgcc gccaggaatt tctggcggca a
//
LOCUS       DAZGCS010000399          830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGCS010000399 DAZGCS010000000
VERSION     DAZGCS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.407"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..687
                     /locus_tag="ACHK01_005502"
     CDS             <1..687
                     /locus_tag="ACHK01_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8100203.1"
                     /translation="KIDDVDIDLVVGFYKNLEDDNDDVLEKRSSDDAGWTVICNDRVV
                     LYCDKTHLTGWGFANVPRFHTQFIAISGVVRFTSKNPEKLPITTTKRGVDLSSTLYND
                     VRNKMIEGMMHFIRFTNQWKGEHLEEGKKLLKSAQSHEAQSLFEITPQSTPEDKKNNW
                     SNPNRNKNEWRYTPKLPTPVKKSSTVRIIFTREKEDVKILSKYFFGHEDASASDVGMQ
                     CFDTVIEEVK"
     gene            687..>830
                     /locus_tag="ACHK01_005503"
     CDS             687..>830
                     /locus_tag="ACHK01_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFO8100204.1"
                     /translation="MSGQYIPYHLRHNKSIDREIFLESLNLLSKRLNIQEYTYIGFGG
                     PMLE"
BASE COUNT          294 a          133 c          163 g          240 t
ORIGIN      
        1 aaaatagacg atgttgatat agatttagtt gttggcttct ataaaaattt agaagatgac
       61 aatgatgatg ttttagaaaa aaggagttct gatgatgctg gatggactgt catttgcaat
      121 gaccgcgttg ttctatattg tgataagaca catctcacag gttggggctt tgctaatgta
      181 ccaaggtttc atacccaatt tatagcaatt tctggggttg ttcgctttac cagtaaaaac
      241 ccagaaaaat taccaataac aacaacgaaa cgaggtgtcg atcttagttc tacattgtac
      301 aatgatgtac gaaataaaat gattgaaggg atgatgcatt ttattcgatt caccaatcag
      361 tggaaaggtg agcatcttga agaagggaaa aaattactta agtcagctca gtcacatgaa
      421 gctcaatctt tatttgaaat taccccacaa tcaactccag aagataaaaa aaataactgg
      481 tccaacccta atagaaacaa aaatgagtgg cgatatacac ctaagctgcc taccccagta
      541 aaaaaaagct ctactgtacg aataatcttc accagagaga aagaagatgt taaaatttta
      601 tctaaatatt tctttggaca tgaagatgct agcgcttctg atgtggggat gcaatgcttt
      661 gatacggtga ttgaagaggt gaagtgatgt ctggacaata cattccctat cacttaaggc
      721 ataataagtc tattgataga gagatttttc tcgaaagcct aaacctctta agcaaaagac
      781 taaatattca agaatataca tatataggtt ttggtggtcc gatgcttgaa
//
LOCUS       DAZGCS010000400          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGCS010000400 DAZGCS010000000
VERSION     DAZGCS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.408"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..460
                     /locus_tag="ACHK01_005504"
     CDS             2..460
                     /locus_tag="ACHK01_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8100205.1"
                     /translation="MSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYALMHSISRSQ
                     NVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLSKAR
                     ISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ"
     gene            504..>826
                     /locus_tag="ACHK01_005505"
     CDS             504..>826
                     /locus_tag="ACHK01_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100206.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNG"
BASE COUNT          278 a          162 c          163 g          223 t
ORIGIN      
        1 tttgagtgga gagacacaag ggctgatttc agcaggttgc tcaacgcttg attctgtcgg
       61 aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa tgcattccat
      121 ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg ttgataaatc
      181 ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat gtgaatttat
      241 actctaccga accagtaaag cgggtattta ccagccttat tataaaataa atctgtcaaa
      301 agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa aagaactgga
      361 accacaggag agaattgcct ttatttatga agatatttcc tgggagcata cgttagccgg
      421 tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa taacataata
      481 aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac acacagaatg
      541 cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata aaccagctag
      601 tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg catcagaaca
      661 ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc ccaataacat
      721 catttgatta cattcatttt atcactacac atccttcagg tataaaagat acactcgcct
      781 ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgg
//
LOCUS       DAZGCS010000401          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGCS010000401 DAZGCS010000000
VERSION     DAZGCS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.409"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..816
                     /locus_tag="ACHK01_005506"
     CDS             7..816
                     /locus_tag="ACHK01_005506"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO8100207.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT          285 a          112 c          162 g          262 t
ORIGIN      
        1 ttaacaatga aagttcaatt gtacaaattc acggaagata aaaataaaac ccttaccttc
       61 agatggacga aaaaacactt tgagttttgt atggataata aaatcttttt aaatcataag
      121 gggaagaagt cgtataaaga aaggaattta tttcttttct caaaaggtga caagataaca
      181 attgaagata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa tttctccagt
      241 gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat aggtcgattt
      301 tgtagtattg catcaaatgt aaaaataatg ggtggaaatc atcctttgaa tcgttttact
      361 actcacatga tgacttataa cggtgaattt gataagtttg ctatgagtga atttgaacgt
      421 agctggacat tgaaaccatt cattaccaaa cctgaaaatc cgattatagg aaatgatgtc
      481 tggataggaa atgatgttgt attgaaaggt ggcatcgcaa taggtgatgg tgctgttatt
      541 gctgccaatt ccgttgttac caaagatgtg cccccatatg ccatagttgc aggagttcct
      601 gcaaaaataa tcagatttag gtttgattca aatgttattg atgaactgtt gagaataaaa
      661 tggtggaatt ataactactc agatcttccg gataataaca agtgtgatga tataaattat
      721 tttgttgaag agatgaatcg attgatatca gatggaaata ttcaagagag ggactataaa
      781 aaattcaatc tttctgaggt attcagaggc ctataattat c
//
LOCUS       DAZGCS010000402          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGCS010000402 DAZGCS010000000
VERSION     DAZGCS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.410"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          224 a          146 c          180 g          261 t
ORIGIN      
        1 aattagagga aatactcctc taatgacgaa caatagattt tcattgattc atcagcaata
       61 gtttttgtgc ctacgaaaag gttgtaatta tttacattaa taatagcatt cagaaaaaat
      121 ttttattgtg ctgataaaat ataccattag tgattagttt agatacctgg ctttcaggca
      181 acttcttttt gtctgtatat aagggatgat tactaaggtg gtacattgat atcagttagc
      241 tttccctttt cgggttactt tatcaaagag tcctctttat agaagagcgc attgtgagtt
      301 ttccagattg acttaaacgt gtggcggcat cacaccgatc aacacgatct gcagcaaagg
      361 gcaggtgtaa ccaggcccat cttagcgcta tgcacttttg agccactgag cttgctaacc
      421 gtttacccgg ctttcactga gctaattacg gcgataatct gatgcccagt aaaacggact
      481 tgatgtatga cgggtcctct tttgcgggat agatatggga atatttctta atgcgaatgg
      541 tacggttatg cgggggggac aaacatggtt gcttgttctg ccatttgttc cttgttcctt
      601 tttataccgc cgccgctccc ggtcagcact ggggaatagg gggttgaggt atatctgctc
      661 cttatgtcag gggaacaggt aatagctata tagcggaacg tcttagtgct gtagttattc
      721 cattttgcta tttgtctaat aggactaatc ttaaagaaaa caaaacgatt aactaaatgg
      781 aaaggcaatt gttaatggcg tttgctaaag a
//
LOCUS       DAZGCS010000403          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGCS010000403 DAZGCS010000000
VERSION     DAZGCS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.411"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /locus_tag="ACHK01_005507"
     CDS             <1..276
                     /locus_tag="ACHK01_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8100208.1"
                     /translation="VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGGATVTPKLNG
                     VAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLMLEINAPAA"
     gene            568..783
                     /locus_tag="ACHK01_005508"
     CDS             568..783
                     /locus_tag="ACHK01_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8100209.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          209 a          165 c          243 g          192 t
ORIGIN      
        1 gtgaatctga tgcatcacag gaaagccgga aacacgaagc tgggggagtt cggcgattac
       61 ggtaacgact ggcagacgct ggagctggtg ttcaccgccg gcggtgccac ggttactccg
      121 aaactgaatg gagtggctgg cccggcattc caggttataa aagacagtct gacactggga
      181 ctgaatgcgc tgacgctgac ggatgttaca aaaaatgcag cgtatggcgt tgagatagaa
      241 agtctgatgc tggagataaa tgcaccggca gcataataaa aaaacagcca gtactgactt
      301 tcgtcggaga agtactggct aagaaggata gttgggtttc acatgatact tatatctggc
      361 agtacatttt ctgacagaca gtgatgggtg ttgtcaagat attgtgtcat ttataacctg
      421 aatcaggggg ggagccggaa tgttatctgg catttttagc agagcctgaa tgccataatc
      481 acggctcccg gcgttggccg tcagtgggcg acactggcgg ctttttgttt tcctttactt
      541 tcattttctg tcggcggtga cggagacata catcagatgg aaaaaatcac aacaggtgtg
      601 tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc
      661 tctccgtccc agtgggtggc aatcggtgtg ctggggagtc tgctgtttgg cctgctgacg
      721 tatctgacta acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggggag
      781 taaagcgatg aagaaaaaat acgaactgg
//
LOCUS       DAZGCS010000404          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGCS010000404 DAZGCS010000000
VERSION     DAZGCS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.412"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..>806
                     /gene="tssD"
                     /locus_tag="ACHK01_005509"
     CDS             37..>806
                     /gene="tssD"
                     /locus_tag="ACHK01_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO8100210.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLL"
BASE COUNT          292 a          132 c          148 g          234 t
ORIGIN      
        1 tgataaaata gcatgccata aaaggacttt tcagggatga gtaatattgt ttacctgaca
       61 gtaacgggag aacaacaagg aagcatctcc gcaggttgtg ggacttctga gtctacaggt
      121 aatcgttggc agagcgggca tgaggatgaa atatttacat tctcactctt aaataatatt
      181 aataatacgg ggcttggttc acagttccat ggtataacat tttgtaaatt aattgataaa
      241 agcactccat tatttattaa ttccattaac aataatgaac aattatttat gggatttgac
      301 ttctatcgaa taaatagatt tggtagatgg gaaaagtatt attatataca actaagaggc
      361 gcttttttat cggctattca tcaccagatc attgaaaacc aactggatac agaaacaata
      421 actattagtt atgaatttat cctctgtcaa catcttatcg caaataccga gttcagctat
      481 ttggcactcc ctgaaaatta taaccgtttg tttttaccaa attcaaaaaa ccaaacaaat
      541 aatcgtttca aaacgttaaa cagcaaagct attggcaggc tacttgctgc tggtggcgta
      601 tacaatggga acattgaagg attcagagat actgcggaaa aactgggtgg agatgcaata
      661 aaaggctatg atcaaatact aaatgaaaaa acagcgggca tagcgatagc aacagcatct
      721 attcttttaa caaagcgttc taatgttgat acatatacag aaataaatag ttacttaggc
      781 aaacttagag gtcaacaaaa acttct
//
LOCUS       DAZGCS010000405          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGCS010000405 DAZGCS010000000
VERSION     DAZGCS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.413"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHK01_005510"
     CDS             complement(<1..275)
                     /locus_tag="ACHK01_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8100211.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSV"
     gene            complement(343..>801)
                     /locus_tag="ACHK01_005511"
     CDS             complement(343..>801)
                     /locus_tag="ACHK01_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100212.1"
                     /translation="ENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVR
                     VYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYS
                     GVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          194 a          219 c          190 g          198 t
ORIGIN      
        1 acggaatctt catgccagcg ggtatcgctg taacgggtaa gctggcgatt cttgtctcct
       61 gcatagctga atgacgtcac cagccccagc gtgtccgtga attcataacg gtatttcacg
      121 ttaatcccgt taagattatc gctaccggga gcgttcgtcc gggcatgaag ataccccgcg
      181 ctcagcgtgg cctgctgctc agacgcccat gcaggcgcac cggatacggt cagacaaatg
      241 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      301 aaaaaaggcg tcatttctga cgcccgttct gggttataaa attcagctga tactgatacc
      361 tgctgtggat tttttcatca ccacaaccag cagatcgctg atacttgctg tgggatacca
      421 gttatttacc agccatgctg acaccgaaaa ctccagcgtc atgtgaccgt gaccggcagg
      481 catatcaata acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc
      541 agcaccgttt ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc
      601 atgtttagcg ccgctgaatg ccaccgccgg aataacaatt tgccggtcaa acggctgatc
      661 gtcataaacc ctgacggtaa tggtccctga tggccaccgc tccggtgcac gggaatcccg
      721 ggggaaagct ttgcccactg ttttaacgag atcgccttca atctggttcg cggacagttt
      781 tcccagaacc cgacagttct c
//
LOCUS       DAZGCS010000406          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGCS010000406 DAZGCS010000000
VERSION     DAZGCS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.414"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..719
                     /locus_tag="ACHK01_005512"
     CDS             <1..719
                     /locus_tag="ACHK01_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO8100213.1"
                     /translation="EYSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARS
                     GDIIGRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKF
                     NKKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKD
                     ISGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLP
                     DESIYVASEPDILAKFVPNK"
BASE COUNT          256 a          163 c          151 g          214 t
ORIGIN      
        1 aggaatactc actttcattt ataaaacaat taccaaatac ggtgaactta tcatctctta
       61 cgtataataa agaggatgat ttcctgtatg ccacccagaa tagccctgcg cagctactta
      121 aaatagccag aagtggcgac atcatcggcc gagccccgct gccttttatc tctgatgccg
      181 aaaccattga gcacatacaa gggaacattt ttgctgcagt agacgaaaag acttctgaac
      241 tattcttttt tacagttaca aaagatatgc atatctcctt gcgaaataaa attcagttag
      301 aaaaattcaa taaaaaaaat agagggtttg aaggactggc atggaaagca gaaggccgga
      361 tgctgtttgt cgctaaagaa cgacgcccta caggcatgtt tgcctatcag ctttctccag
      421 atctgctgag tgcaaaacaa gtaacgattc cagaggaact aaatgatata catgttaaag
      481 atatatccgg cctggacttt aataatgaaa gtctgatgat tctgtccgat gaatcgcgta
      541 agttactcaa gtttaacctg acagaaatgt ctttcgttga gatgatggac ctgacaaaag
      601 gaaatcattc tctgaccagt gatttgctcc aacctgaagg cattgtaact ctgcctgatg
      661 aaagcattta tgtcgctagt gaacctgata tcctcgctaa atttgtccct aataaataag
      721 tctgtcacta gccttagtat tgagcgacca aatctggcaa tctcatgaaa ctcgcaaaat
      781 acga
//
LOCUS       DAZGCS010000407          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGCS010000407 DAZGCS010000000
VERSION     DAZGCS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.415"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..736)
                     /locus_tag="ACHK01_005513"
     CDS             complement(32..736)
                     /locus_tag="ACHK01_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO8100214.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAZGCS010000408          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGCS010000408 DAZGCS010000000
VERSION     DAZGCS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.416"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..276
                     /gene="yddL"
                     /locus_tag="ACHK01_005514"
                     /pseudo
     CDS             1..276
                     /gene="yddL"
                     /locus_tag="ACHK01_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            315..>768
                     /locus_tag="ACHK01_005515"
     CDS             315..>768
                     /locus_tag="ACHK01_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO8100215.1"
                     /translation="MITDLILHNNPRMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRN"
BASE COUNT          273 a          155 c          132 g          208 t
ORIGIN      
        1 atgaaattaa aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac
       61 gctgccgaag tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct
      121 ctacgttatt ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc
      181 tttaaaggag aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat
      241 tttaaaggct ataacgatga agccaacggt tcgcgcggca acaatctctg attgagttaa
      301 gtgcccatca caatatgatc actgacctta tattacacaa taatcccagg atgaaaacaa
      361 tcactttaaa cgacaaccat attgcacatt taaacgccaa aaacactaca gaactggaat
      421 atttaaactt aagcaataac aatttactgc caaccaatga cattgatcaa ctaatatcat
      481 caaaacatct ttggcatgta ttagttaacg gcatcaacaa tgatccactt gcacaaatgc
      541 agtactggac tgcagtaaga aatataattg atgacactaa tgaagtgacc attgatttat
      601 caggacttaa tttaaccact caaccaccag ggctgcaaaa cttcacctct atcaatcttg
      661 ataataacca actcacacat tttgatgcaa ccaactacga tagactcgta aagctaagtc
      721 tgaatagtaa tactcttgag tcaataaatt ttcctcaagg cagaaatg
//
LOCUS       DAZGCS010000409          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGCS010000409 DAZGCS010000000
VERSION     DAZGCS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.417"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHK01_005516"
     CDS             complement(<1..467)
                     /locus_tag="ACHK01_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8100216.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
BASE COUNT          217 a          185 c          187 g          178 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct cccccagttc aaaccaggtc
      241 accacacctt tcacccggcg ctgtacgtca tcgccctgcc ataccgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggaa agaaactgct gactgaccag agaaaggtcg
      361 agggaaaaaa gtgaagagag tgactgggtg agatgaaagg aaaccaccgc aaacgcgtcc
      421 ggtggcaggc cgtccacttc aagtgtgaaa cgtaatccgg ttgacatagc aacctccgtg
      481 aaaatgaata ataatccgca catattgcat gtacaacatg tcgggcggat gagataagcg
      541 agtcgacatc tggcgttacc ggggaatatc agaaaaatgt catggttatg tgatgattta
      601 gtctgggatt ttacttcaga gaaacgggtg tctgtttagt caacaaaata atcacaaacg
      661 caacggatgg gttctcgacg ttattcacga caggctcgta cattacagcg agtaataaaa
      721 aacctgatat gcaaagtcaa accaatattc ataatgtctt tttattc
//
LOCUS       DAZGCS010000410          762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGCS010000410 DAZGCS010000000
VERSION     DAZGCS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.418"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(191..>762)
                     /gene="escD"
                     /locus_tag="ACHK01_005517"
     CDS             complement(191..>762)
                     /gene="escD"
                     /locus_tag="ACHK01_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO8100217.1"
                     /translation="NDILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLN
                     TPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILA
                     IINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILK
                     GGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT          212 a          152 c          133 g          265 t
ORIGIN      
        1 ccttatttag gtaaatttct ttacggcgag gccattattg aattgcccaa ctgcgcttgt
       61 ttaatattct gtataaatgc ttctctcaga actgaccata tttttccacc gctccctatt
      121 agaaaatttg ctattacttc accctacaat aaataaaaaa ccctccgaag agggggaaga
      181 cgataataaa ttaatacgat agcggaatat gtatatattc gtctgggcgg gagatgttga
      241 taccatcttt aggatcaatc gcgtctatga catagcctcc tttcagaatg ctaccaattc
      301 ccaggcttgt accgttatca agagcaattg ttggattctt catcgtgccg ctgattctga
      361 gtatttttcc ggggaaaata tctgctgaaa tgtacggctg aatgttctga aatagtatct
      421 tgacgttact atttttgttc atcgcattta taatggcaag gatcttactc tcgttctgtt
      481 gagaaacctc ccccgcaaca atgatgacat cattcttttt gaaaatattg acatagttta
      541 ttaatttatt ctttttgaac tctgatgcca gttcattgat tttattgtta ctgttgttat
      601 gtatttccca tccagcaacc cctggcgtat tcagtaacag attttcaact tcgctccatt
      661 tgggtgaagg gggaatgtat cccgataaca ggaaaaatcc aggcttatta ccttttgtaa
      721 tgatgatatc cttataaccg tattccgtta aaatatcatt aa
//
LOCUS       DAZGCS010000411          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGCS010000411 DAZGCS010000000
VERSION     DAZGCS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.419"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..669
                     /locus_tag="ACHK01_005518"
     CDS             <1..669
                     /locus_tag="ACHK01_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO8100218.1"
                     /translation="DARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQE
                     LNRTETAREVQRPSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGE
                     KTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALY
                     MPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHN
                     PVAA"
BASE COUNT          184 a          189 c          246 g          138 t
ORIGIN      
        1 gatgcgcgca tgctggcaga gttctgtcgt cagaagcgcc ctgcagcctg ggaagcgcct
       61 cacccgcttg aacgcgcgtt gcgtgccctg gtagtccgcc accaggcgct gacagatatg
      121 cacacgcagg aactgaatcg cactgaaacg gcgcgggaag tccagagacc gagcattgat
      181 gctcaccttc tgtggcttga agcagagctg aagcgtcttg agaagcagat aaaagacctg
      241 acagacgatg atccggatat gaaacaccgc aggaaactgc tggaaagcat cccgggtatc
      301 ggagagaaaa catctgcggt attgctggct tatatcggtc tgaaggaccg cttcgcccat
      361 gccagacagt tcgccgcttt tgcgggtctg acaccacggc gttatgaatc aggtagcagt
      421 gtgagagggg cgagccggat gagtaaggcc ggacatgtgt cgcttcgcag ggcgttgtat
      481 atgcccgcaa tggtagccac cagtaagact gagtggggac gggcgttccg cgaccgtctg
      541 gcggctaatg gcaagaaagg aaaggtgatt ctcggcgcga tgatgcgcaa gctggcacag
      601 gtggcgtatg gagtgctgaa gtcaggcgtg ccgttcgatg cgtcacggca taatccggta
      661 gcggcgtaaa aatcgcggaa gggatgaaaa aaacagcgcc tgacggcgct gtgtctggca
      721 tgcctgcaat ccgggaaacc ggaccaggaa aaaactt
//
LOCUS       DAZGCS010000412          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGCS010000412 DAZGCS010000000
VERSION     DAZGCS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.420"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..390)
                     /locus_tag="ACHK01_005519"
     CDS             complement(46..390)
                     /locus_tag="ACHK01_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO8100219.1"
                     /translation="MTEADLYPYLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(387..>755)
                     /locus_tag="ACHK01_005520"
     CDS             complement(387..>755)
                     /locus_tag="ACHK01_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO8100220.1"
                     /translation="NRVLREATRAGANVLKEEVVSRAPVRRGKLRRNVVVLSRRSRDG
                     GMESGVHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFVRPAFDVRSE
                     QAAQVAIARMNRAIDEVLRR"
BASE COUNT          168 a          253 c          193 g          141 t
ORIGIN      
        1 cccgcacgcg gcggggtatg ggttttgtag aaaaaaaaga aaaagtcaca ccgtgacctg
       61 aaactccagg gttgcacggt aacagcggtt ttccggaata tagtcctgca tttcactgac
      121 ggatcccggg gccagcagca ttatggcttc acgggcgtcc tgacgtatct gacgcgcctg
      181 cgtcacagtc ccggcataaa cgtctatctg caccgacact gaggactccg cctgcccgcc
      241 catcacgtcc gcagacaccg atgaaatcag gctgaaaacc acccacggaa gcgccaccga
      301 cggcctgcca tccagcaggg ggaccacata cgggtacacc tgcccgccgg caagatgcgc
      361 cagataagga tacaaatccg cctccgtcat cgtctcagta cctcatcaat ggcccggttc
      421 atccgcgcaa tcgccacctg agctgcctgt tcactgcgca catcaaacgc cgggcgcaca
      481 aacgggtgcg gtggcatatt cacggtcccc atttccacaa accgccagta gaaagcattg
      541 cgcgggttat ccgccttcat ggtgttatcg ctgttaccgg tgtccggatt aacaccacgg
      601 atatggacac cggattccat cccgccatcg cgggagcgcc gggaaaggac caccacattg
      661 cggcgcagtt ttcccctgcg taccggtgcc cgtgacacca cttcttcttt cagcacattc
      721 gcacccgcac gggttgcctc acgcagcacc cggtt
//
LOCUS       DAZGCS010000413          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGCS010000413 DAZGCS010000000
VERSION     DAZGCS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.421"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>743
                     /locus_tag="ACHK01_005521"
     CDS             <1..>743
                     /locus_tag="ACHK01_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO8100221.1"
                     /translation="RSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQR
                     YHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHISE
                     GHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAV
                     EWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNS
                     LLSDRDYTWNDNGELIRISSPRQTRSYSY"
BASE COUNT          175 a          241 c          221 g          106 t
ORIGIN      
        1 cgcgcagtat ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca
       61 gccacaccac cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg
      121 gccgcacaca gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg
      181 ggctggtcac ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg
      241 gtgaaaccgc agagcggtgg cggtacgacg aacgcggctg gctgacagac atcagccata
      301 tcagcgaagg gcaccgggtg acggtgcatt acgggtatga tgagaaaggc cgcctgaccg
      361 gtgagcgtca gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca
      421 gacatgcgta caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg
      481 tggaatggct gacctacggc agcggctggc tgtcaggcat gaaactcggc gacacaccgc
      541 tggtggagta cacccgcgac cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg
      601 aactcaccac cgcttacacc cctgccgggc agttacagag ccagcacctg aacagcctgc
      661 tgtctgaccg cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc
      721 gccagacccg gagttacagc tac
//
LOCUS       DAZGCS010000414          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGCS010000414 DAZGCS010000000
VERSION     DAZGCS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.422"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..215
                     /locus_tag="ACHK01_005522"
                     /pseudo
     CDS             69..215
                     /locus_tag="ACHK01_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          226 a          124 c          142 g          249 t
ORIGIN      
        1 ctcgttaatg attggtcttg ctcattgtta ataacaaaat gttgaattta ccgattagga
       61 aactttttcc tacgcggatt gcctccagct atggcatgaa ctttgagttt atgccagaac
      121 tgaagtgggg cttcggctac cctggcgcga ttatctttat gatcctcgcg ggcctgacat
      181 cgtatctgta ctttaagcgt aagaactggc tgtaactata cgatgtcgct gtgagtaagt
      241 tagtgtgctg acagcgacat tttagccgag ggaatcgcag ataacataat tgttgtgaat
      301 aatatccatg ccacgttgag ttggacgctt aatgactcac atctcacccc ttcaattgct
      361 gcagagataa tagaaaaaag tgtcaagccc aatgaaatca atacaaagta gattgcctta
      421 catccagtat ctcttattct ttttgctcca atattgatat ttacataggt attattaata
      481 accagaataa aactcaaagc caggttgata tcattcaaag ttattaatat tgaccatgat
      541 gttaatgtat agattatgaa ctgaaagcgt gataaacgct caagcctgga tgggtagaat
      601 atatttctga tggatgattt gaacattttt acattgtttt atatataatt gttttgaata
      661 tatattacct gaattatgtg gtttgcatat atatgcaata tagcagtctt tgcattgata
      721 actatacagt agtaatttag a
//
LOCUS       DAZGCS010000415          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGCS010000415 DAZGCS010000000
VERSION     DAZGCS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.423"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..>731
                     /locus_tag="ACHK01_005523"
     CDS             43..>731
                     /locus_tag="ACHK01_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8100222.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VSEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          242 a          125 c          164 g          200 t
ORIGIN      
        1 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
       61 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      121 ggtgtagaaa tctatgctgc tgctaaacaa ggcgaacccg atccagaatt aaacacatct
      181 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      241 gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg ttatgaaggc
      301 tttggtccta atggctcaat gattatcgct gagacattga cttcaaatgt taaccgtacg
      361 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      421 gtcagctata tgtttgacaa tacgggtgtg attgtattta aagggacaga ccctgaccat
      481 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgagtga agaagaaggt
      541 aacattgtta tttatactga acctactgac cttcataaag gaatcgcggc tctaaaagca
      601 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      661 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      721 gatgtacaaa a
//
LOCUS       DAZGCS010000416          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGCS010000416 DAZGCS010000000
VERSION     DAZGCS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.424"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>724)
                     /locus_tag="ACHK01_005524"
     CDS             complement(<1..>724)
                     /locus_tag="ACHK01_005524"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8100223.1"
                     /translation="SKNEGGHIRMLVYRLTDFLCDATTNVSNEALDVFIKKKAFSKSK
                     SIPVYNGIDTDLFKYDPLQRDKIRHSLNIINDDSLILSVGRLTDAKDYPNLLKALLEL
                     PERYKLVIIGEGEARNEVERTILNYGLEKRVKLLGSIANVATYYSACDIYVSSSKWEG
                     FGLVVAEAMSCQRLVVATNAGGVAEVVGDMNYIVPVSDPQLLANKINEVMEFDHETKM
                     EVMYRNREFVLKMFSIDMIVNQW"
BASE COUNT          241 a          155 c           92 g          236 t
ORIGIN      
        1 ccattgatta acgatcatat ctattgagaa catttttaaa acaaactctc tatttctata
       61 cattacttcc attttagttt cgtgatcaaa ctccataact tcattaattt tatttgccaa
      121 caactgaggg tctgaaacag gtacaatata gttcatatca ccaacaactt cagccacccc
      181 accggcatta gttgcaacga ccaatcgctg gcaagacata gcctcagcta caaccaaacc
      241 aaagccttcc cacttagagg acgatacata aatatcacat gcagaataat aagtggcgac
      301 attagcaata ctacctagca atttcactct tttttcaagg ccgtaattta aaatcgtacg
      361 ctcgacttca tttctggctt caccttcacc tattatgact aatttatatc gttcaggtaa
      421 ttctaacaat gcttttaata agtttggata atcttttgca tcagtaagtc ggcctacaga
      481 aagtataagg ctatcatcat taatgatatt taaactatgc cttattttat ctctttgtaa
      541 aggatcatat ttaaatagat cagtgtcaat gccattataa acgggaattg acttcgattt
      601 gctaaaggct tttttcttaa taaaaacatc aagagcttca ttacttacat tagtcgtagc
      661 atcacataaa aaatcagtta atctataaac gagcatccta atatgcccac cttcattttt
      721 acta
//
LOCUS       DAZGCS010000417          720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGCS010000417 DAZGCS010000000
VERSION     DAZGCS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.425"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..>720
                     /locus_tag="ACHK01_005525"
     CDS             134..>720
                     /locus_tag="ACHK01_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO8100224.1"
                     /translation="MIDFLNLKRINAQYEVELKDACSRVIDSGWYIMGTELKTFEEKF
                     ANWCGVKHVIGVANGLDALILVLRAWKEMGKLNDGDEIIVPANTYIASVLAITENNLV
                     PVLTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYGQISPMAEIMEIAAEFGLLVLED
                     CAQAHGSSIDGRKAGSWGHAAGFSFYPGKNLGALG"
BASE COUNT          234 a           93 c          168 g          225 t
ORIGIN      
        1 taatcgcatt tcataaaaaa acaggagcca ccataataaa agaggatgac gtgaattttc
       61 actttgaatt ttatgtggaa aattttattc gtttaagaga aaaatatagt aaatattttt
      121 gaggggaaag aaaatgattg attttttgaa tttaaaaaga ataaatgctc aatatgaagt
      181 ggaattaaaa gatgcttgct ccagagttat tgattctggt tggtatatta tggggacgga
      241 gttaaagaca tttgaggaaa aatttgcaaa ttggtgtgga gttaagcatg ttattggtgt
      301 tgccaatggc ttagatgcac taatccttgt tcttcgggct tggaaagaaa tggggaaatt
      361 aaatgacgga gatgaaatta ttgtacctgc caatacatat attgcatctg ttttggcaat
      421 aactgaaaat aatctagtcc ctgtattgac tgaaccagat cctttgacat tcaacttaag
      481 tgcgggtggg attcgtaaag taattacagg gaaaacaaaa gcaattttgc cagtgcattt
      541 gtatggacaa atatctccaa tggcagaaat aatggagatt gcggcagagt ttggtttatt
      601 ggttttagag gattgcgctc aagctcatgg ttcctctatc gatggacgca aagctggcag
      661 ttggggtcat gcagctggat tcagtttcta tccaggaaaa aatcttggcg ctttaggtga
//
LOCUS       DAZGCS010000418          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGCS010000418 DAZGCS010000000
VERSION     DAZGCS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.426"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..700
                     /gene="fimE"
                     /locus_tag="ACHK01_005526"
     CDS             104..700
                     /gene="fimE"
                     /locus_tag="ACHK01_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO8100225.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          181 a          143 c          178 g          212 t
ORIGIN      
        1 actccgtgtg tggttatctt tttatctatt gggctaattt tgaccgattg aggtttccta
       61 taggtattca ttcaaatata tctcagttag gagtactact attgtgagta aacgtcgtta
      121 tcttaccggt aaagaagttc aggccatgat gcaggcggtt tgttacgggg caacgggagc
      181 cagagattat tgtcttattc tgttggcata tcggcatggg atgcgtatta gtgaactgct
      241 tgatctgcat tatcaggatc ttgaccttaa tgaaggtcga ataaatattc gccgactgaa
      301 gaacggattt tctaccgttc acccgttacg ttttgatgag cgtgaagccg tggaacgctg
      361 gacccaggaa cgtgctaact ggaaaggcgc tgaccggacc gacgctatat ttatttctcg
      421 ccgcgggagt cggctttctc gccagcaggc ctatcgcatt attcgcgatg ccggtattga
      481 agctggaacc gtaacgcaga ctcatcctca tatgttaagg catgcttgtg gttatgaact
      541 ggcggagcgt ggtgccgata cccgtttaat tcaggattat ctcgggcatc gaaatattcg
      601 ccatactgta cgttataccg ccagtaatgc tgctcgtttt gccggactat gggaaagaaa
      661 taatctcata aacgaaaaat taaaaagaga agaggtttga tttaactcat tgat
//
LOCUS       DAZGCS010000419          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGCS010000419 DAZGCS010000000
VERSION     DAZGCS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.427"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /gene="ykfM"
                     /locus_tag="ACHK01_005527"
     CDS             complement(<1..153)
                     /gene="ykfM"
                     /locus_tag="ACHK01_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFO8100226.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWR"
     gene            complement(163..>710)
                     /locus_tag="ACHK01_005528"
     CDS             complement(163..>710)
                     /locus_tag="ACHK01_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100227.1"
                     /translation="YFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRG
                     ALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGS
                     LASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQEL
                     DKWKRENMTPFMYVLNTPPVI"
BASE COUNT          238 a          145 c          118 g          209 t
ORIGIN      
        1 tctccaatca taaaatattt ccgtggagca ttttattatt gaatatagag gtttaactcc
       61 ggtaaaaaac aaagaagcat tgaatgcagg gaaaaataat atggccataa aaaacatcga
      121 aagaaactct tttaatttaa catgtaaacg catggttaat cctcatatca cgggtggagt
      181 gttaagaaca tacataaatg gagtcatgtt ttcccttttc catttatcaa gttcctgttg
      241 ccgttttagt ccatctctaa ttgcatattt taatttttct gataaatggc attgagcatc
      301 gatttcattt aaaacaactg tacacactat ccctgtcagt aaaattgctc ctccgacaag
      361 aattactgga aagccaaagc taacacaggc aaaagaagcc aggcttccaa tagcggttgc
      421 aacaccaccg gctataactg ctttggccac atccattgaa aaatcgccaa gaaaattaac
      481 cagatctcgc tctgaactca taataaattc tatggttctc caggctactg aaaaccagat
      541 acagaactta actcctttta ttgattcaga taaagctcct cgccagccaa tagcgagttc
      601 cagaacttga ggcgctcgtg aagagaaaat atatttaccg agcttttcta aaagccaggg
      661 gctggatgtt gtgattttta tatatttctc tccaagtatt tcaaagtaac
//
LOCUS       DAZGCS010000420          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGCS010000420 DAZGCS010000000
VERSION     DAZGCS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.428"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   87..603
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=87..115
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          181 a          186 c          181 g          162 t
ORIGIN      
        1 tggatgtgtt gtttgtgtga tactataaag ttggtagatt gtgactggct taaaaaatca
       61 ttaattaata ataggttatg tttacagagt tccccgcgcc agcggggata aaccgccctc
      121 cagtactgtg atgcgatctt taagtccgag ttccccgcgc cagcggggat aaaccgactg
      181 gttctgtaac ccaaatttgg gatcattcga gttccccgcg ccagcgggga taaaccgact
      241 atcggcgctg ttcagttggg acagaaacag agttccccgc gccagcgggg ataaaccgtt
      301 atcgcaccgt gttaaaccgg cagaaaaaca gagttccccg cgccagcggg gataaaccgc
      361 gtgccttcga ctttgcggat cacgtcgtct tgagttcccc gcgccagcgg ggataaaccg
      421 acggggacga atcacagcag cgttatcgtg atgagttccc cgcgccagcg gggataaacc
      481 gaaaccggac gagggcgcgc taaaacgtga ttagagttcc ccgcgccagc ggggataaac
      541 cgcctctcaa tttgtaacgt ctgcaatgtc aggcgagttc cccgcgccag cggggataaa
      601 ccaccatata acccgttatc tctttctcaa gtttttatat tagcagtact tgtaataagc
      661 aacatatcca cgtaacacct catgttcaaa atagttctcc atgccagaga
//
LOCUS       DAZGCS010000421          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGCS010000421 DAZGCS010000000
VERSION     DAZGCS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.429"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /locus_tag="ACHK01_005529"
     CDS             <1..117
                     /locus_tag="ACHK01_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8100228.1"
                     /translation="EPETQRVIYLREGYEHECFSPLEQFRRKFREIEVGHEH"
     gene            107..>708
                     /locus_tag="ACHK01_005530"
     CDS             107..>708
                     /locus_tag="ACHK01_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100229.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWK"
BASE COUNT          190 a          169 c          190 g          159 t
ORIGIN      
        1 gagccggaaa cacaacgagt tatctacctc cgcgaaggct atgagcatga gtgcttcagc
       61 ccgctcgaac agtttcgtcg taaattcagg gaaatagagg tcggtcatga gcactaaatt
      121 aaccggctat gtatgggatg gttgcgctgc atcaggcatg aaattatcca gcgtggcaat
      181 tatggcccgc ctggctgatt tcagtaatga cgaaggtgtg tgctggccat caattgaaac
      241 tattgcccgt cagattggcg cggggatgag taccgtcagg acggctatcg cacggctgga
      301 agcagaaggc tggttaacgc gtaaggcgcg tcgccagggt aaccgcaatg cgtcgaatgt
      361 ttatcagctt aacgttgcga agcttcaggc agcggcattt tctcaactgt cagattctga
      421 cccgtcaaaa tctgacgcat caaaatctga cccgtcaaaa tttgatgcgt cgaaatctgg
      481 caaaaaagcg ggttttcacc cgtcagaatc tggcggggat ccgtcagtaa aatcaaaaca
      541 tgatccgtca gataaaaaac cttctcgtcc ggacgcttcg caaccggaca cgcagacgga
      601 tgaacaggat tttttaactc gccatcctga tgcggttgta ttcagcccta aaaagcgcca
      661 gtggggaacg caggatgatt tgacctgcgc acagtggctc tggaaaaa
//
LOCUS       DAZGCS010000422          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGCS010000422 DAZGCS010000000
VERSION     DAZGCS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.430"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHK01_005531"
     CDS             complement(<1..467)
                     /locus_tag="ACHK01_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8100230.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
BASE COUNT          193 a          173 c          177 g          161 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc
      241 accacgcctt tcacccggcg ctgtacgtca tcgccctgcc atatcgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg
      361 agggaaaaga gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcgtcc
      421 ggcggcaggc cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt
      481 aaaatgaata atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca
      541 gacgttatta gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt
      601 actttacaga aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt
      661 tcttggcgtt attcaggaca ggtacgcata tcacaacgag taac
//
LOCUS       DAZGCS010000423          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGCS010000423 DAZGCS010000000
VERSION     DAZGCS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.431"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>699
                     /locus_tag="ACHK01_005532"
     CDS             <1..>699
                     /locus_tag="ACHK01_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO8100231.1"
                     /translation="FVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFI
                     WGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGY
                     NALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHI
                     EKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVDLNS
                     SKYGYYEKVGGGFY"
BASE COUNT          236 a           85 c          153 g          225 t
ORIGIN      
        1 agtttgtcca tgaaattaat aatatatcat ctgctcgctg gatgtcaaaa tcagaaaaga
       61 ataataaaca aaaagaggca atagaaaagt tcaaaaaaga agtatcccat atgaatggcg
      121 ggcagcagtt tatttggggg tttgataagg ttattcaaga aggcttaagt ggattgattg
      181 agttaagtat cgatattaat gatagtacaa atcatcgtga taagtcttct ctttctcctg
      241 atgggagagc tgtgttacat tttttaggta caatttggaa tatggcgatg ggagctgtac
      301 ctggttataa tgcattgtct ggtgtaagta gcattttaca tagtgctata gtaaaagaat
      361 ctagcaatat ctgtgattat attcaggggg ctgtacgtat tggaatggac tttgttccag
      421 gcactcgctc tgacttacat agccgttcgc tgcagataaa atatgaggct ttgaagcata
      481 tagaaaaaaa cattaatgat aatattattt atcatccgag taataatgct aatttctatt
      541 ctgtaattga gtcaattgat ggtaatgact ttatatataa cgaaaaacaa tctaaaatat
      601 tagaaatgaa acaggatcgt ggggggaata gatatagtgc agtagatctt aactcttcta
      661 agtatgggta ttatgagaaa gttggcggtg gtttttata
//
LOCUS       DAZGCS010000424          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGCS010000424 DAZGCS010000000
VERSION     DAZGCS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.432"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>696
                     /locus_tag="ACHK01_005533"
     CDS             <1..>696
                     /locus_tag="ACHK01_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100232.1"
                     /translation="QIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYHIHSFLTQIP
                     DNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLI
                     ATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSILATLY
                     KMLISEREVSEYECQLLYLTNPIDVMNILNKYIYYFPNENSPFYTLKIDSALSWDAID
                     AIRDYSISDIYLYP"
BASE COUNT          255 a          125 c          105 g          211 t
ORIGIN      
        1 caaataataa aacaaaaata ttttaatgac attgtaccaa tgattctcgc actcgacatc
       61 aggaacacat atcgcccgga taatgagatg gcattttact atcatattca ttcttttctc
      121 actcagatac cggataatga ggatgatata tatcatgctg caaggacata tctgcgaaat
      181 tacgttaagt tatgtttatc cggatacacg ccagcgaatg cgcatttcaa agatatcttt
      241 gatggcgtat atgaattcat tcgtaatatt cgcaaaaaca gtacaccagg aaaaacaaaa
      301 cttatcgcaa ctatcaacac atgcaaagaa acctgtaaac atctgcttta tttaagtaat
      361 gaagacaagg aaaaaataat ttctgactta gataaagttc aggttgcatg ttattatctc
      421 actatattac tggctttcga aagacgaact tcattaacaa gcatcctggc aactttatat
      481 aaaatgctga taagcgaaag agaagtttca gaatatgaat gccagttatt atatttaacc
      541 aacccaatag atgtaatgaa tatactgaac aaatacatat attactttcc taatgagaac
      601 tcaccatttt atacactgaa aattgacagt gcattatcgt gggatgccat tgacgcaata
      661 cgagactata gtatttctga tatttatctt tatcct
//
LOCUS       DAZGCS010000425          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGCS010000425 DAZGCS010000000
VERSION     DAZGCS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.433"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>696
                     /locus_tag="ACHK01_005534"
     CDS             <1..>696
                     /locus_tag="ACHK01_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8100233.1"
                     /translation="AELHEAKKNIPPKNPVDADKLLTASRGEFVEGISDPNDPKWVKG
                     IQSRDDDDQNQDKAEQNSPNALQNEPKTKQPEPVVQQEPEKVCTTCGQTDGGNCPDCG
                     AVMGDATYQETFNEESQDEARKKNPEEMENAVLPNKQCTEGDQHANGNNETGETANPL
                     ITVNGHHVITSTSRTCDHLMIDLKTMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIK"
BASE COUNT          249 a          157 c          164 g          126 t
ORIGIN      
        1 gcagaactcc atgaagccaa gaaaaacatt ccacccaaaa atcctgtcga tgcagataaa
       61 ttactgactg cttctcgagg agaatttgtt gaagggatta gcgacccgaa tgatccaaaa
      121 tgggtgaagg ggatccagag tcgcgatgat gatgaccaga atcaggataa agcggaacaa
      181 aacagcccaa atgcgttaca aaacgagcca aaaacaaaac aacctgaacc agtagtgcaa
      241 caagaaccag aaaaagtttg caccacatgt ggtcagaccg atggcggcaa ctgccctgat
      301 tgtggagcgg taatgggtga cgcaacctac caagagacat tcaacgaaga aagccaggat
      361 gaagcccgga aaaaaaatcc ggaggaaatg gaaaatgccg tgctcccgaa taagcaatgc
      421 accgaaggcg atcaacatgc caatggcaat aatgaaacag gcgagacagc aaatccctta
      481 attacggtga acggtcatca cgttatcaca tccaccagca ggacgtgtga ccatctaatg
      541 atcgacctta aaaccatggg aaaaaatcct gatgccccga ttatctcaat aggtgcaata
      601 tttttcgatc cgcaaaccgg agatatggga ccggaattta gtaagactat cgatctggaa
      661 actgctggcg gagtcattga tcgggacacc attaaa
//
LOCUS       DAZGCS010000426          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGCS010000426 DAZGCS010000000
VERSION     DAZGCS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.434"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(463..675)
                     /locus_tag="ACHK01_005535"
     CDS             complement(463..675)
                     /locus_tag="ACHK01_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO8100234.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
BASE COUNT          233 a          141 c          107 g          214 t
ORIGIN      
        1 atacgctacc gccccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
       61 cactggctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatatc
      121 ataagcaaat agcatagaaa ataatgacaa ttcccccgag gggaccattt ccaaagcact
      181 gcaccaccac ctggtgcccg gatgatcttg cttcagtggt aagagtacct gattcattgt
      241 tcccaaaatg atgttttccc cggccccgcc aataatgtgc agctctcacg tgataatcta
      301 ttgattaggt tatattaaga atcaacattt aaaatacatc cattttcttg aataaatcaa
      361 catgtttatt aatcaattgc tgttttagct ctgactcatc aagctctcca tatttgtcct
      421 acagataatt gcgaataaat acagagaacg ccatttatcc ttttagcata cctgccggat
      481 atttttattt gtattaatat taattgcatc tccaactccg agcatatcca tcattaaaaa
      541 ctttttgaga ttatagaata cctgtgataa caagtgaaac atctgcacaa tgtgaatagt
      601 tagtttcttt tcagaggaaa tgaaatagac aagctgaatg aaggggagag tgatataaat
      661 caaaaaacat aacatgaatt taattaatta cgccc
//
LOCUS       DAZGCS010000427          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGCS010000427 DAZGCS010000000
VERSION     DAZGCS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.435"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>686
                     /locus_tag="ACHK01_005536"
     CDS             <1..>686
                     /locus_tag="ACHK01_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100235.1"
                     /translation="RQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRR
                     LKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWSLRKD
                     DYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEASRLKYTEEAQ
                     KAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPSAVKVS
                     AGERQEDRAHA"
BASE COUNT          192 a          157 c          225 g          112 t
ORIGIN      
        1 cgccagttcc ataacgtgac ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc
       61 ggcgatgaag ccggggcatt gcaggcggcg aacgaggccg cgacgaaagg atttgatgac
      121 cagacccgcc gcctgaaaga gaacatgggt acgctagaga cctgggcaga caggacagca
      181 cgggcattca aatccatgtg ggatgcggtg ctggatattg gtcgtcctga taccgctcag
      241 gagatgctga ttaaggcaga ggctgcgttt aagaaagcgg acgacatctg gagtctgcgc
      301 aaggatgatt attttgttaa cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag
      361 gcccgtcttg cgcttgaagc cgcgagaaag aaggctgaac agcagagtca acaggacaaa
      421 aatgcgcagc agcagagcga tactgaagcg tcacggctga aatataccga agaggcgcag
      481 aaagcttacg aacggctgca gacgccgctg gagaaatata ccgcccgtca ggaagaactg
      541 aacaaggcac tgaaggacgg gaaaatcctg caggcagatt acaacacgct gatggcggcg
      601 gcgaaaaagg actatgaagc gacgctgaaa aagccatctg ctgtgaaggt gtctgccggt
      661 gagcgccagg aagaccgggc gcatgc
//
LOCUS       DAZGCS010000428          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGCS010000428 DAZGCS010000000
VERSION     DAZGCS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.436"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>682
                     /locus_tag="ACHK01_005537"
     CDS             <1..>682
                     /locus_tag="ACHK01_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100236.1"
                     /translation="ERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKH
                     TSPKTRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLK
                     EAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSA
                     KQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAALAKATSALKNTWSAEEQLRGSW
                     MAGLKSGWG"
BASE COUNT          180 a          169 c          237 g           96 t
ORIGIN      
        1 cggaacggga tgcgcgtata cgccaggggg aaatggggta catcaaccgc tcgcgtgcaa
       61 caggcgtcag caaaggtcca gggcagcagg aagccgtcag ccgtctggct gaagagctga
      121 caggtaaaaa gcatacatca ccgaaaacgc gctctgccgg ggagagggaa gaggagcagg
      181 caagagaggc tctgcttgcc cttgaagctg agctcaggac gctggaaaaa cacagcggtg
      241 cgaatgagaa aatcagccgg cagcgccgtg atttatggaa ggcggaaagt cagtatgcgg
      301 tcctgaaaga ggctgccacg aaacgacagt tatctgagca ggaaaaatcc ctgctggcgc
      361 ataaagacga gacgctggag tacaaacgcc agctggctga gctgggcgac aaggttgaat
      421 accagaaacg cctgaatgag ctggcacagc aggcggtgcg gtttgaagag caacagagcg
      481 cgaagcaggc cgccatcagc gcaaaagccc gcggtctcac tgaccgtcag gcgcagcggg
      541 agtctgaagc gcagcgtctt cgggacgtgt acggtgataa tccggctgcg ctggcgaagg
      601 ccacatcggc actgaagaac acctggtctg cggaggagca gcttcgtgga agctggatgg
      661 ccgggctgaa gtccggctgg gg
//
LOCUS       DAZGCS010000429          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGCS010000429 DAZGCS010000000
VERSION     DAZGCS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.437"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..349
                     /locus_tag="ACHK01_005538"
     CDS             170..349
                     /locus_tag="ACHK01_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8100237.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            390..635
                     /locus_tag="ACHK01_005539"
     CDS             390..635
                     /locus_tag="ACHK01_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8100238.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT          185 a          153 c          194 g          146 t
ORIGIN      
        1 ggtggaagtg aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat
       61 ggacgctggc ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct
      121 ggtttttaat gaaaacagtt cttattgtca acaataacgg aaagaaatta tgacatttct
      181 gaaccagtta atgctgtact tctgtacggt ggtctgtgtg ctgtatctcc tttcgggtgg
      241 gtacagggca gtgcgcgatt gctggcgcag gcagattgac aaaagggccg ctgagaaaat
      301 cagcgccggt cagtcagccg gaagcaaacc cgaagagccg ctcatttagc ggcaactttc
      361 ttaatcacac ctttcgacga gaaaatccca tgtcagaaat tacatccctg gtcactgctg
      421 aagcagtgaa ggaagtcctg cgctctgaag aagtccggag cgcactgaaa cagaaacttc
      481 gccataacct ggaagcgcgt cttgatgcag aagtggatgc cattctggat gaactgctgg
      541 gcgcaccggc agctccggag ccggaaggca tcgcgggtga ggggagtgct tcagatagcg
      601 gtgaccccac accggacagc gacatgatga tgtaagcatg cgtcagggac catcggtgtg
      661 tgccggtggt ctttttta
//
LOCUS       DAZGCS010000430          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGCS010000430 DAZGCS010000000
VERSION     DAZGCS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.438"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>663
                     /locus_tag="ACHK01_005540"
     rRNA            <1..>663
                     /locus_tag="ACHK01_005540"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ctccttgaga gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAZGCS010000431          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGCS010000431 DAZGCS010000000
VERSION     DAZGCS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.439"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>660
                     /locus_tag="ACHK01_005541"
     CDS             <1..>660
                     /locus_tag="ACHK01_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100239.1"
                     /translation="QGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRS
                     AGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQ
                     LSGQEKSLLAHEKETLEYKRQLADLGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISA
                     KARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADEDMLRGDWLAGLKSG
                     WG"
BASE COUNT          170 a          167 c          232 g           91 t
ORIGIN      
        1 ccagggggaa atggggtaca tcaaccgctc gcgtgcaaca ggcgtcagca aaggtcctgg
       61 gcagcaggaa gccgtcagcc gtctggctga agagctgaca ggtaaaaagc atacatcacc
      121 gaaaacgcgc tctgccgggg agagggaaga ggagcaggca agagaggctc tgcttgccct
      181 tgaagctgag ctcaggacgc tggaaaaaca cagcggtgcg aatgagaaaa ttagccggca
      241 gcgccgtgat ttatggaagg cggaaagtca gtatgcggtc ctgaaagagg ctgccacgaa
      301 acggcagtta tccgggcagg aaaaatccct gctggcccat gagaaagaga cgctggagta
      361 caaacgccag ctggctgacc tgggcgacaa ggttgaacac cagaaacgcc tgaatgagct
      421 ggcacagcag gcggtgcggt ttgaagagca gcagagcgcg aagcaggccg ccatcagcgc
      481 aaaagcccgc ggtctcactg accgtcaggc gcagcgggag tctgaagcgc agcgtcttcg
      541 ggacgtgtac ggtgataatc cgcaggcgct ggcccgggtc accggggcac tgaaacagac
      601 atgggcggat gaagacatgc tgcgcggtga ctggctggcc gggctgaagt ccggctgggg
//
LOCUS       DAZGCS010000432          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGCS010000432 DAZGCS010000000
VERSION     DAZGCS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.440"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..192
                     /locus_tag="ACHK01_005542"
     CDS             <1..192
                     /locus_tag="ACHK01_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8100240.1"
                     /translation="LSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAY
                     QQQIAESRRPEEETTPRET"
     gene            182..>660
                     /locus_tag="ACHK01_005543"
     CDS             182..>660
                     /locus_tag="ACHK01_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8100241.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     "
BASE COUNT          139 a          169 c          222 g          130 t
ORIGIN      
        1 ctgagcacgt atgagaaaga gctggcgctg atgggcgagg attatcagga cattttccgc
       61 cagcaggtca gggaatctgc agagcggcaa aaagccggac tctcacgtcc ggtgtggata
      121 gcgcaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga gacaacacca
      181 cgtgagacgt aatctttcac acattattgc cgcagcattc aatgaaccgc tgcttctgga
      241 gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgc gagatggggg cagcaagtct
      301 ttcggtacca caacagcagg tacagtttga tgctcccgga atgctggctg aaacggacga
      361 gtacatggcc ggaggtaaac gaccggcccg tgtttacagg gtggtgaacg gtattgctgt
      421 actgccggtg accggcacgc tggtgcaccg gctggggggt atgcggccat tttccggaat
      481 gacaggctat gacggtattg tcgcctgtct tcagcaggca atggcggata gccaggtgcg
      541 gggcgtactg ctggacattg acagtccggg cgggcaggcc gccggcgcgt ttgactgcgc
      601 tgacatgatt taccgcctcc gtcagcagaa gccggtctgg gcactgtgca atgacacggc
//
LOCUS       DAZGCS010000433          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGCS010000433 DAZGCS010000000
VERSION     DAZGCS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.441"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>656
                     /locus_tag="ACHK01_005544"
     CDS             <1..>656
                     /locus_tag="ACHK01_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8100242.1"
                     /translation="GTLNDFLGAMSEDDVRPEALRRFELMVEEAARHAEEAKKNAGEA
                     ETSARNAGISASQAEESAANADTSAGEASESARQAAESAASAKQSEEASSSSASAAAQ
                     KASESSQSAAEAELSRKTAESAAGNAARDATTAAEKARESAESAQSAEQSRIAAEEAV
                     NRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPAGATGERGPAGDAGPAGPQGPK
                     G"
BASE COUNT          169 a          166 c          244 g           77 t
ORIGIN      
        1 ggtacgctga atgattttct cggtgccatg tcggaggatg acgtccggcc ggaggcactg
       61 cgtcgttttg aactgatggt ggaagaagcg gcgcgtcacg ctgaggaggc gaagaagaat
      121 gccggagagg cggagacgtc cgcgaggaat gccggcatat cagccagtca ggcagaagag
      181 agcgcggcaa atgctgacac ttcagcaggg gaggcatcgg agtcagcccg gcaggcggca
      241 gaaagtgcag cctcagcaaa gcagtcagag gaggcgtcct cgtcctcggc ttctgcggcc
      301 gctcaaaaag ccagtgagtc atcacaaagt gcagcagaag ctgaattgtc aagaaagacg
      361 gcagaaagtg cagccggtaa tgcagccagg gatgcaacga ccgcagcaga aaaagcccgg
      421 gagtcagcag aaagcgcaca gtcagcggaa caaagcagga tagcggcgga agaagccgta
      481 aaccgaatcc cgaccgtggt ggggcctccc gggccaaagg gggaaccggg tcccgcgggt
      541 cctcaggggc cgaagggaga taaaggagag cgtggcgaca ccggcccggc aggggcaacc
      601 ggtgaaaggg ggccggcagg tgatgctggt ccggcaggcc cgcaggggcc gaaagg
//
LOCUS       DAZGCS010000434          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGCS010000434 DAZGCS010000000
VERSION     DAZGCS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.442"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..510
                     /locus_tag="ACHK01_005545"
     CDS             85..510
                     /locus_tag="ACHK01_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8100243.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHK01_005546"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHK01_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          186 a          162 c          160 g          147 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccttcac cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atcca
//
LOCUS       DAZGCS010000435          645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGCS010000435 DAZGCS010000000
VERSION     DAZGCS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.443"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..101
                     /locus_tag="ACHK01_005547"
     CDS             <1..101
                     /locus_tag="ACHK01_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO8100244.1"
                     /translation="FAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          200 a          113 c          143 g          189 t
ORIGIN      
        1 aattcgctgt aaataacggt aatattgatg caaagagcaa acttcaagag ctcaaaaagc
       61 taaagcgaat ggatagaagg aagaataagg agaatccgta aaagcaatag ttcatcagac
      121 tgccgcagca gtagtatgat gatgaatcag ggctgtacta taactattat cggtaacatt
      181 aaccgcagca ggggcggtat aacactcagg ctccgattgg gctggaaggt tggtggaatt
      241 tttatcagta tccgttgaac ccgatttctg gaatcgatcc ccttgggatt agccacctgt
      301 ttatacagta taactttagg tatgctttcg tgtgtttcag atataccatg tgatgataat
      361 ttttatgatg tgcttagtat tccagtcgct tctggtaata atatgcaatg taaaaataat
      421 ccccgttgta cacatttata aatagatggc caatacctca aggcctatgg agttagaatg
      481 ttaatgaccc gggagcaagc aatagaaaac ctgacggcat acgattagta ccgtcagtta
      541 atacaggaac ttataatcgc aatggatttt caatacatag ttgtttaaac gcttttggtc
      601 cgtctttagg acctcgattc tgttccgagg gttgtataac tggtt
//
LOCUS       DAZGCS010000436          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGCS010000436 DAZGCS010000000
VERSION     DAZGCS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.444"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..529
                     /locus_tag="ACHK01_005548"
     CDS             <1..529
                     /locus_tag="ACHK01_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8100245.1"
                     /translation="MASYAFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKP
                     PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESV
                     KRGMPVIRDCQRCGGRGYERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFD
                     IEEAWAEQQLKKVTR"
BASE COUNT          174 a          114 c          181 g          172 t
ORIGIN      
        1 tatggcttcg tatgcgtttg aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc
       61 ctgttgtcac ggaaaaaaat ttattgaaag cgaggttttt acaaacaaga tccagtatcc
      121 ggatggtaag ccgccagtgt gggcaaagtg cacaaaaggc gtgtatccgt cttactggga
      181 ggaatggaaa aaagtcaggg aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg
      241 ggaggtttcc accgcctgta aagattgtcg tgggcgcggt gttgccattc atcgtgaaga
      301 gtcggtaaaa cgtggtatgc ctgttatcag agactgccag cgttgtggtg gtcgtggcta
      361 tgaaagatta ccttcaacgg aggcatttaa tgccatatgt aatgtaaccg atgccatatc
      421 tcttgataca tggaaaaaaa cagttaaacg tttttacgat acgctggtgg tgcagtttga
      481 tattgaagaa gcatgggcag aacaacaact gaaaaaggtg accagatagc tttgttgatt
      541 tttcccgaat ctgtggtaaa tttgccctaa cgatgggcgt tttatgcctg acgttagaag
      601 attttttaca cccgtcgcca ggcgggtttt tttatgactg a
//
LOCUS       DAZGCS010000437          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGCS010000437 DAZGCS010000000
VERSION     DAZGCS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.445"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHK01_005549"
     CDS             complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHK01_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFO8100246.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRC"
BASE COUNT          195 a          132 c          136 g          177 t
ORIGIN      
        1 cgcaacgtcc ataaagcgcg acatttttcc catgccatac cagagtgatt gcatgaagtt
       61 tgttttcttt tagctgctgg tatacatcag ctctggtaaa cgctgggggt tcagcttttg
      121 gggaaagcaa aacgtaactg cctatggcgc cagacaacgc aatcgcagca ccaatcacgc
      181 tagccaggat aagacgcttt ttacgtcgat ttttggcaga gaacctttct ggcacatcag
      241 acaatggcaa ggtgtggtca atatcaccaa tgatgaagca acatcccgct atatcgatga
      301 cctttccgac aggtaatgac tgatctgctt cataaggggt gggctgtcca tcaatccaaa
      361 attcaaccgg agataaaagg aaaacacctt cctcacgtat atcaagtaaa aactgattga
      421 ggccttgttc aaggggaaaa taaacaatat cgttatcttc tgtgcctatc gttaggtttc
      481 ccatcggaag ttgtaattcc cgattacgca tcgcaccatt gagaagtttt attttatatg
      541 aggataacat ttttaaattt tattcatcct ggtggttgaa caatgagaaa aaatatggtg
      601 aacttacatc aactaagaat agagtagaaa ggaagctgtt
//
LOCUS       DAZGCS010000438          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGCS010000438 DAZGCS010000000
VERSION     DAZGCS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.446"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>637
                     /locus_tag="ACHK01_005550"
     CDS             <1..>637
                     /locus_tag="ACHK01_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO8100247.1"
                     /translation="PDSRILSSVRDAVASSDNGAQVKVGNRTYRVVVTDNKFCVTRES
                     HSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTMERGAVHSNRPDLPPVDYAPPEL
                     PSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKMFTASQSNEEVTNEVRCFNQYYG
                     AGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQAEHAIYDMFDRLEQKG"
BASE COUNT          190 a          100 c          170 g          177 t
ORIGIN      
        1 tcctgacagt cgtattttat cctctgtaag ggatgcagtt gctagctcgg ataatggggc
       61 gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc actgataata agttttgcgt
      121 tacaagagaa agtcatagtg gttgctttac taatctgttg cacaggctgg gatggcctaa
      181 gggagagatt agcaggaaaa ttgaggtgat gctgaattca tcgccagtga ataggactat
      241 ggaaagaggt gctgttcatt cgaatagacc tgatttacct cctgttgatt atgcaccgcc
      301 agagttaccg agtgtggact ataacaggtt gcctgtgcct ggtaatgtta ttggcaaagg
      361 gggtaacgct gtagtatatg aagatgctga ggatgcaaca aaagtcctga agatgtttac
      421 tgcatctcaa agcaatgaag aggtaacaaa cgaagttcgt tgcttcaacc aatattatgg
      481 tgccgggagt gcagaaaaaa tatatggcga taatggcgat attattggta ttagaatgga
      541 taaaataaat ggagaatcgc ttttaaatat ttcgtccttg ccggcacagg ctgagcatgc
      601 catttatgat atgtttgaca ggcttgagca aaaagga
//
LOCUS       DAZGCS010000439          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGCS010000439 DAZGCS010000000
VERSION     DAZGCS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.447"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..366)
                     /locus_tag="ACHK01_005551"
     CDS             complement(<1..366)
                     /locus_tag="ACHK01_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8100248.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMG"
BASE COUNT          199 a          115 c          127 g          188 t
ORIGIN      
        1 acccataggc agcattgaag tgattgaacc atgaatcagg gagttgctga tgttcaactg
       61 gtttgttgtt ccatcagtaa agtcatgatt caggtaatat gtagagatag tcccgttaac
      121 agtgtcacca ttaaaggtgg tatcgaaatc accgtttgtg ttgttatagt agacaaagcc
      181 actataattc acatagttac catctacagt acccggtgca tccatattgc tccagtcaag
      241 agcggtagtt ggattgtcgt aataagtcca ggtgtcataa gtagttgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaatgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataacctat ggagaaaaac acgcatcaaa
      541 ttgataaaag ggcgatattt caacatgttg cagataatgt tatttttgat acatacgcgt
      601 tataaatatc aaaaacacaa accagataa
//
LOCUS       DAZGCS010000440          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGCS010000440 DAZGCS010000000
VERSION     DAZGCS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.448"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>620
                     /locus_tag="ACHK01_005552"
     CDS             <1..>620
                     /locus_tag="ACHK01_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100249.1"
                     /translation="RLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAESD"
BASE COUNT          174 a          141 c          201 g          104 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgacctcgg ttttgtgggt gatccgtatc aggaggcgag
       61 acaacgttat atcagtaatg ccctggaagc ctggcgcaat aacgaggcga ataaacccaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaaa gagcaactgg cactggccgg tcagaacacg gagctggcga agctgaagta
      241 ccagacagcg ctgggtgaac tgaaaaccct gtcggagata cagaagcagg agctgctgcg
      301 taacgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa gccgaactgc tggggtacgg
      421 gcagggagaa cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggcg ctggcgcaga atgcacagta tctgagtgaa cgtctgaaag agcaggaagc
      601 cttttatgcc gaatcggatg
//
LOCUS       DAZGCS010000441          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGCS010000441 DAZGCS010000000
VERSION     DAZGCS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.449"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>620
                     /locus_tag="ACHK01_005553"
     CDS             <1..>620
                     /locus_tag="ACHK01_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100250.1"
                     /translation="RLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRNA
                     ALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAESD"
BASE COUNT          176 a          141 c          200 g          103 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgaccttgg ttttgtgggg gattcgtatc aggaggcgag
       61 acagcgttat atcagtaatg ctctggaagc ctggcgtaat aacgaggcga acaaacctaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctactgaa
      181 gcagcagaaa gagcaactgg cactggcggg tcagaataca gagctggcga agctgaagta
      241 ccagactgcg cagggcgaac tgaaaaccct gacggagatg cagaagcagg aactgctgcg
      301 taacgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa gccgaactgc tgggatacgg
      421 gcagggagaa cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggca cgggcacaga acgcgcagta tctgagcgaa cgccttaagg accaggcagc
      601 cttttatgcc gaatcggatg
//
LOCUS       DAZGCS010000442          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGCS010000442 DAZGCS010000000
VERSION     DAZGCS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.450"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            347..>618
                     /locus_tag="ACHK01_005554"
     CDS             347..>618
                     /locus_tag="ACHK01_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO8100251.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEG"
BASE COUNT          209 a          117 c          129 g          163 t
ORIGIN      
        1 gtacaataga tgtcatctta ggttctaggt ttataaaaga actgaaagaa agcaggtctt
       61 tctctcgtgt agagataagc gagtcagagt tatggggtat atcaaataat cctgtaaacc
      121 acgctcacca acgaaaccca aacaaaaagt ggtcataacc cgctcaggcg gatttcattt
      181 tcacgcaaac aacagaataa acacagcgct gtgtattcat tccaacgagt gaatacacgg
      241 agcaatgtcg ctcgtaacta aacaggagcc gacttgttct gattattgga aatcttcttt
      301 gccctccaag atgagggcga ttttttatct gtgaggatat gaacagatgt caaacatcaa
      361 aaaatacatc attgattacg actggaaagc atcaatagaa attgaaatcg accatgacgt
      421 aatgacagag gaaaaacttc accagattaa taatttctgg tcagactctg aataccgact
      481 caataaacac ggctctgtat taaatgctgt attaatcatg ctggcgcaac atgctctgct
      541 tatagcaatt tcaagcgact taaatgcata tggtgttgtg tgtgagttcg actggaatga
      601 tggaaatggt caggaagg
//
LOCUS       DAZGCS010000443          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGCS010000443 DAZGCS010000000
VERSION     DAZGCS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.451"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>613
                     /locus_tag="ACHK01_005555"
     CDS             <1..>613
                     /locus_tag="ACHK01_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100252.1"
                     /translation="ATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPE
                     SSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAAT
                     LQSDMEKAGELAARDRAERDASQLKYTGEAQKGYERLLTPLEKYTARQEELNKALKDG
                     KILQADYNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDQAHA"
BASE COUNT          168 a          148 c          203 g           94 t
ORIGIN      
        1 tcgccacgaa aggctttgat gagcagaccc gtcgcctgaa agaaaacatg gggacactgg
       61 agacctgggc ggataaaaca gggaaggcat tcaaatcgat gtgggatgcc attctggata
      121 tcggtcgtcc tgagtcctca gcggatatgc tcgccagtgc gcagaaggca tttgatgagg
      181 cggataaaaa atggcagtgg taccagagcc ggagccagcg ccgcggtaaa acctcttctt
      241 tccgggccaa ccttcagggc gcatggaatg accgggaaaa tgcccggctg gggctggcag
      301 cggccacgct gcagtcggat atggaaaaag ccggtgaact ggccgccagg gaccgggccg
      361 aacgggacgc atcacagctg aagtataccg gagaggcgca gaaggggtat gagcgtctgc
      421 tgacgccgct ggagaaatat accgcccgtc aggaagaact gaataaggcc ctgaaagacg
      481 ggaaaatcct gcaggcggat tacaacacgc tgatggcggc ggcgaaaaag gattatgaat
      541 cgacgctgaa aaagccgaag tcgtcaggag tcaaagtgtc agccggtgag cgtcaggaag
      601 accaggcgca tgc
//
LOCUS       DAZGCS010000444          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGCS010000444 DAZGCS010000000
VERSION     DAZGCS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.452"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>612
                     /locus_tag="ACHK01_005556"
     CDS             <1..>612
                     /locus_tag="ACHK01_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO8100253.1"
                     /translation="NMFREVSISNDTISVKFYRNEKIECACNFLMDKDAQGYIDLSDM
                     NLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGYCNFTTATVDNVIFRCRRLHNVIFI
                     KASGECVDFSKNILDTVDFSRSQLTHSNFRECQIRNSNFDHCYLYASHFTRAEFLSDK
                     EISFIKSNLTAVMFDHVRISTGNFKDCITERVELTIDYSDIFG"
BASE COUNT          201 a           84 c          114 g          213 t
ORIGIN      
        1 taaatatgtt tcgtgaagtc tcaattagta atgatactat aagtgtaaaa ttctacagaa
       61 atgaaaaaat agaatgcgct tgtaattttc tgatggataa agatgcgcag gggtatatcg
      121 acctgtctga tatgaattta acaagttgtc attttaaagg tgacgttata tcgaaggtgt
      181 cttttttatc atcaaatcta caacatgtaa cattcgaatg taaagaaatt gggtattgca
      241 attttactac tgcaacagtt gataatgtta tatttagatg tcgacgttta cacaatgtga
      301 tttttatcaa agcgagtggt gaatgtgtcg attttagcaa aaatattctt gatacagttg
      361 acttctcgcg gagccagctt actcatagta attttcgcga atgtcagatt agaaattcaa
      421 acttcgatca ttgttatctt tatgcttcgc acttcaccag agcagaattt ctttctgaca
      481 aagaaatatc atttatcaaa tcgaatttga cagctgttat gtttgatcat gtgcgaatat
      541 cgacagggaa ttttaaagat tgcattacag aacgagtgga attaactatt gattattcag
      601 atatatttgg ga
//
LOCUS       DAZGCS010000445          606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGCS010000445 DAZGCS010000000
VERSION     DAZGCS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.453"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            365..580
                     /locus_tag="ACHK01_005557"
     CDS             365..580
                     /locus_tag="ACHK01_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8100254.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          157 a          112 c          170 g          167 t
ORIGIN      
        1 ttcacagaca gcaatgatgc ctgtcaatat attatcaata tgcggattgt ttcagttaca
       61 gatgctttat taaggaaaaa aacagccagc actgactttc ggtggagagg tgctggctca
      121 gaaggatagt tggatttcac atgatactta tgcctggcgg tatattttct gacagacagt
      181 gacgggtgtt gtcaagatat tgtgtcattt ataacctgaa tcaggggagg ccggaatgtt
      241 atctggcatt tttagcagag cctgaatgcc ataatcacgg ctcccggagt tggccgtcag
      301 tgggtgacac tggcggtttt tttgtttttc tttactttca ttttctgtcg gcggtgacgg
      361 agacatacat cagatggaaa aaatcacaac gggtgtgtca tacaccacgt cagcggtggg
      421 gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt gggtggcgat
      481 aggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgacaaacc tttatttcaa
      541 gattaaagaa gataagcgta aggcggcgcg gggagagtaa agcgatgaag aaaaaatacg
      601 aactgg
//
LOCUS       DAZGCS010000446          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGCS010000446 DAZGCS010000000
VERSION     DAZGCS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.454"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>595
                     /locus_tag="ACHK01_005558"
     CDS             <1..>595
                     /locus_tag="ACHK01_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO8100255.1"
                     /translation="RRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGT
                     LNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTA
                     ITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQI
                     TAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLG"
BASE COUNT          125 a          175 c          187 g          108 t
ORIGIN      
        1 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
       61 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      121 cagtcaggca ccctgaacag cgtcatccct ccggcagtgc agcacctgac ggtggaggtg
      181 agcgcagctg acggccagta tctggcgcag gcgaaatggg acacgccgcg ggtggtgaag
      241 ggcgtgcgct tcagtctgcg cctgaccagt ggaagcggtg aggacagccg tctggtgacc
      301 accgccatca ccgcggatac agagcaccgt ttcagcggtc tgccgccggg ggaatacacc
      361 ctgacggtca gggcgattaa cagttatggc cagcaggggg aaccggccac caccacgttc
      421 aggattaatg cacctgcggt acccgccacg attgagctga caccgggcta ttttcagata
      481 acagcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg
      541 gagacaaaaa tcgcagacac atctcaggtg gaaacctctg cccgttatct gggga
//
LOCUS       DAZGCS010000447          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGCS010000447 DAZGCS010000000
VERSION     DAZGCS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.455"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..279
                     /locus_tag="ACHK01_005559"
     CDS             <1..279
                     /locus_tag="ACHK01_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100256.1"
                     /translation="GAARHAKMLETDQAWEVFEKLEDSYFNQYEKERAMGKMNIDFHL
                     AIRDDKTAFIRSYAPGQLVTVEEALALLKSRGWLVMPRDELAGKLMEL"
BASE COUNT          170 a          124 c          162 g          129 t
ORIGIN      
        1 ggagcagccc gtcatgcaaa aatgctcgaa accgatcagg cgtgggaagt attcgaaaaa
       61 ctcgaagaca gttacttcaa ccagtacgag aaagagcggg ctatgggaaa aatgaatatc
      121 gattttcacc tggcgatccg tgacgataaa accgccttca tccgaagtta tgcgcctggc
      181 cagctggtga cggttgaaga agccctggct ttgcttaaat cgcgcggatg gctggtaatg
      241 ccacgagatg aactggccgg aaaattaatg gagctttaac cgattgagtt aaccttgata
      301 tgggaattcc catatcggga gaacattacc atcggggata aaatgatttg cggggaaaga
      361 agagttaagt agaattgctg cgggtgcttg aggctgtctg cctcgggcat gccgccgtaa
      421 ggcagacaga gaaaagcccc agttaacatt acgcgtcttg caggacgctt aacattaatc
      481 tgaggccaat ttcatgcttt gcacatgtag gttagcctct tacgtgccga aaggcaagga
      541 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgat
//
LOCUS       DAZGCS010000448          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGCS010000448 DAZGCS010000000
VERSION     DAZGCS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.456"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>566
                     /locus_tag="ACHK01_005560"
     CDS             <1..>566
                     /locus_tag="ACHK01_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO8100257.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYAD"
BASE COUNT          100 a          190 c          175 g          101 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccgat
//
LOCUS       DAZGCS010000449          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGCS010000449 DAZGCS010000000
VERSION     DAZGCS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.457"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..234)
                     /locus_tag="ACHK01_005561"
     CDS             complement(43..234)
                     /locus_tag="ACHK01_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100258.1"
                     /translation="MNKSKNPVCWLQTGFYVYGSKLWEAALIDFSKLIRELRLMISQL
                     PNWKFLLVWSIPFLWVVSQ"
     gene            complement(312..>565)
                     /locus_tag="ACHK01_005562"
     CDS             complement(312..>565)
                     /locus_tag="ACHK01_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO8100259.1"
                     /translation="VPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEA
                     IRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT          145 a          153 c          111 g          156 t
ORIGIN      
        1 ttatgagttt gtctgacata gctacccctt aattgccaca attcactggg atactaccca
       61 taaaaaaggg atgctccaga ccagcaaaaa tttccagttt ggtaattgac taatcatgag
      121 tcgcaactcc ctaatcagtt tgctaaaatc aatcaaggca gcctcccata gcttactgcc
      181 ataaacataa aaccccgttt gcaaccaaca aacggggttt ttacttttat tcacttgggt
      241 tttgccagtt cgcaggattt cgtgttatct gcccgcgttg gccaacgtca tttttaagca
      301 aaatattctg cttatctgtc gataccccag cacgccagcg cactttcctg atctcgccgg
      361 gatacctgac cgtagcaatt atttgagcgg atacggcagt ctctgccacc gtccttaatc
      421 caccagcgaa tcgcttcgca ggcacctttt cgatcacctg cattaattcg tttataaaac
      481 gtcgacggga aacacttacc ggggccaatg ttatacggac agaatgacgc gatccccgct
      541 ttctggggtt cggtcagcgg cactt
//
LOCUS       DAZGCS010000450          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGCS010000450 DAZGCS010000000
VERSION     DAZGCS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.458"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..290
                     /locus_tag="ACHK01_005563"
     CDS             <1..290
                     /locus_tag="ACHK01_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100260.1"
                     /translation="LHMPSNNIIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPP
                     LIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            257..>562
                     /locus_tag="ACHK01_005564"
     CDS             257..>562
                     /locus_tag="ACHK01_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein"
                     /protein_id="HFO8100261.1"
                     /translation="MVIKQRKCSGIKKRDNSRDKTKEHKHMNRKFYFKNILWGMCPII
                     ISILHLIYDTPTEASIKVDLFLILSGLALPCSIYLTKTITLKYTSISFWKKPLFWSYG
                     "
BASE COUNT          221 a          117 c           70 g          154 t
ORIGIN      
        1 tattacacat gccatcaaac aatataattg aaataggcta caatataata aacaccgatc
       61 tggtcattgg cgaaaaaatt catttcagga cactaagcaa tcgttcttta tattttaacc
      121 tcacccaacc cccattaatc gccacaataa aagatgatgt gttttgcact cttcatgatt
      181 actataacca caataatgaa acgaaatcca ctattgacaa ttacatatca aaaataaagg
      241 ataatcataa taccccatgg ttattaaaca acgaaaatgt tcaggaataa aaaaaagaga
      301 caattcccga gataaaacaa aagagcataa acatatgaac cggaaattct atttcaaaaa
      361 tatcctgtgg ggaatgtgcc ccattataat atccatattg catctgatat atgacacacc
      421 aacagaagca tcgataaaag ttgacttatt tctgatactc agtggcctgg cccttccttg
      481 ttcaatatac ctgacaaaaa caatcacact aaaatacaca tccatatcat tctggaaaaa
      541 acctcttttc tggtcttatg gt
//
LOCUS       DAZGCS010000451          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGCS010000451 DAZGCS010000000
VERSION     DAZGCS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.459"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..>559
                     /locus_tag="ACHK01_005565"
     CDS             15..>559
                     /locus_tag="ACHK01_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8100262.1"
                     /translation="MTTPVISFINMKGGVGKTTLCVGITEYLANFMDKKVLIIDIDPQ
                     FNATQSILSKYDRVKEYIADHLENTKTIRRIFETKSSLTGKVQQVTPDEIIIKMSEHL
                     DMIFGDINTIFDIEQPAARLHKLKRFIEEHKLRDIYDFILIDSPPTISLFTDAALVAS
                     DYYIVPVKIDHYSILGATSLL"
BASE COUNT          203 a           92 c           96 g          168 t
ORIGIN      
        1 attagaggga taaaatgact acaccagtaa taagtttcat aaatatgaaa ggtggggttg
       61 gtaaaacaac tttatgcgtc ggcattactg aatatcttgc aaacttcatg gataaaaagg
      121 tattaattat cgatattgac ccacaattta acgctacaca gtcaatactt agtaaatatg
      181 atcgagtgaa agagtatata gctgatcact tagaaaatac aaaaaccatc agacgaatat
      241 tcgagacaaa atcaagctta acgggtaaag ttcagcaggt cactcctgat gaaattatca
      301 tcaagatgtc agagcactta gatatgattt ttggtgatat taatacaata tttgatattg
      361 aacaacctgc ggcaagatta cataagctaa aacgctttat agaagaacat aagcttcgag
      421 atatatatga cttcattttg atcgatagcc caccaaccat ttcattattt actgatgcag
      481 ctctcgttgc ttcagattat tatattgtgc cagttaaaat tgaccactac tcaattttag
      541 gtgcgacaag tcttttaag
//
LOCUS       DAZGCS010000452          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGCS010000452 DAZGCS010000000
VERSION     DAZGCS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.460"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..179)
                     /locus_tag="ACHK01_005566"
     CDS             complement(<1..179)
                     /locus_tag="ACHK01_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100263.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWV"
     gene            339..>558
                     /locus_tag="ACHK01_005567"
     CDS             339..>558
                     /locus_tag="ACHK01_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8100264.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEK"
BASE COUNT          143 a          107 c          133 g          175 t
ORIGIN      
        1 ttaacccatg cgttgatatt atgtttcagc cgttgaagca tcagcggtgt taatgttgtg
       61 gtaataatat ccagcgtttt atgtgagatc ttaccgtaag ggtctgcaag aatgctgctt
      121 gttgcttcgt tattatctgc catcagaaga agtaactctg atttaacgtt ttctgtcatt
      181 agttgtaaaa atcttctgcg caaactttct ttactgttca tttatatggc ttcatttgtt
      241 gtaatctgct gcgtctcaag ggatatgttt atgagagcga ccatgagtgt tggattatat
      301 acctaacata tcaagggatt agaaatcgat aaatccccat gaacgaaaaa ataaaatacg
      361 gcctgtcggc tgccgttctg gcgctgattg gtgcaggtgc ttctgcgcct gaaatcctcg
      421 accagtttct ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcgggga
      481 tctggaccat ctgccgtggt gccattctgg tggatggtaa gcctgttatt cctggcatga
      541 agctgtcaaa ggaaaaat
//
LOCUS       DAZGCS010000453          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGCS010000453 DAZGCS010000000
VERSION     DAZGCS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.461"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..487
                     /locus_tag="ACHK01_005568"
     CDS             272..487
                     /locus_tag="ACHK01_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8100265.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKVARGE"
BASE COUNT          147 a          109 c          149 g          150 t
ORIGIN      
        1 cagcgcccac tctgaaggac gctggctaaa acgggtagat gtacttcaca tgatacttat
       61 atttggcagt acattttctg acagacagtg acggatgttg tcaagatatt gtgtcattta
      121 taacctgaac caggggggcc ggaatgttat ctggcatttt tagcagagcc tgaatgccat
      181 aatcacggct cccggagttg gccgtcagtg ggtgacactg gcggcttttt gttttccttt
      241 actttcattt tctgtcggcg gtgacggaga catacatcag atggaaaaaa tcacaacagg
      301 tgtgtcatac accacgtcag cggtagggac gggatactgg ttactgcaac tgctggacaa
      361 agtctctccg tcccagtggg tggcaatagg tgtgctgggg agtctgctgt ttggcctgct
      421 gacgtatctg acaaaccttt atttcaagat taaagaagat aagcgtaagg tggcgcgggg
      481 agagtagtcg atgaataaac aatacgaact ggttgtaaaa tgaatatttc taactgaaaa
      541 aacgttccat gaggt
//
LOCUS       DAZGCS010000454          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGCS010000454 DAZGCS010000000
VERSION     DAZGCS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.462"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..181
                     /gene="dicB"
                     /locus_tag="ACHK01_005569"
     CDS             <1..181
                     /gene="dicB"
                     /locus_tag="ACHK01_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8100266.1"
                     /translation="LSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEVCIP
                     SMLARLDLLQKGYKQ"
     gene            178..369
                     /locus_tag="ACHK01_005570"
     CDS             178..369
                     /locus_tag="ACHK01_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8100267.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            463..>539
                     /locus_tag="ACHK01_005571"
     CDS             463..>539
                     /locus_tag="ACHK01_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO8100268.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVAT"
BASE COUNT          173 a          110 c          113 g          143 t
ORIGIN      
        1 gttatcatgt aatgccagca cgtctgaact tcgtttcgaa attggcgtta tcactggaga
       61 caaaacattt attgaagacg ccattaagca gagaaaactc gagcaggacc tgttaaatga
      121 agtatgcatt ccttcaatgc tggctcgtct ggacctgctg caaaaaggat ataaacaatg
      181 aatacaacat ttgcactcgt tctgacagtt tatcttgttt ccggcgaatc tcttgagctg
      241 gtgactggct tatacggttc aatgaaagaa tgcatggctg cagcagcaga acaaaaaatt
      301 cccggtaact gttatccggt agataaaact actcacacta ataataacga aataccggca
      361 ggactttaaa acagcaccgt aattaatatc cggtttcatt tttatatgcc agcaatggca
      421 gggatttgtt cacccttaaa tctgtaatga ggttaaaaca aaatgagtaa agtctttatt
      481 tgcgccgcca ttccggacga acaggcaata aaagaagagg gcgcagttgc tgtagccac
//
LOCUS       DAZGCS010000455          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGCS010000455 DAZGCS010000000
VERSION     DAZGCS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.463"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>538)
                     /locus_tag="ACHK01_005572"
     CDS             complement(<1..>538)
                     /locus_tag="ACHK01_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8100269.1"
                     /translation="QTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGTHKVDGNQFE
                     ALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLADEL
                     VNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLATML
                     AGQQGMSVEQARAILAAAA"
BASE COUNT          103 a          180 c          134 g          121 t
ORIGIN      
        1 tgccgccgcg gccagaatcg cccgggcctg ttcaacgctc atgccctgtt gtcctgccag
       61 catcgtggca agctgttcac gtcctttcgc ttcctggcat gtcaggatcc ccatcactcg
      121 ctggttctcc tgcgcggcgg cttccgttgc agttaattgc ggcatagtgc ctcctctgac
      181 attactgttc agcgccgtgg ccatcacact gatggcatcc gacgcattga ctaattcatc
      241 cgccagcccg gcatcaatgc cggactgacc ttcaaaaacg gcggcctctg ttcccgtgac
      301 ggcatcaaca gacagaccgg taaacatcgc cactttttcg gcaaacatcc ggcgcgccgc
      361 atcaatgcgc tgctgcatgt cctggcgaac ctctgccggt aaggcttcaa actgattgcc
      421 atccaccttg tgcgtccctg agtaaatcag cgtgatatcc acaccggcct gcgccagatg
      481 accggcatag ctgacatggc tcatcatcac gccaatggag ccgatacggg atgtctgg
//
LOCUS       DAZGCS010000456          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGCS010000456 DAZGCS010000000
VERSION     DAZGCS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.464"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /locus_tag="ACHK01_005573"
     CDS             <1..339
                     /locus_tag="ACHK01_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFO8100270.1"
                     /translation="WKTSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAG
                     GACANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGA
                     GVFSVFGYQT"
     gene            complement(336..>538)
                     /locus_tag="ACHK01_005574"
     CDS             complement(336..>538)
                     /locus_tag="ACHK01_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100271.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          137 a          119 c          128 g          154 t
ORIGIN      
        1 tggaagactt ctggttcgct caatggttct tacacaaact taggttcaca tagaggttca
       61 ttctcagggc ggaattcagg gggcagtaca ttgtttattt atgcatctgg aggtaatgga
      121 ggatctgccg gaggtgcatg tgcaaataca tcccgactgc agggatatgt tggtgggacg
      181 ctaattagcg tgaatgccag caataaccct gcatatggaa aaacagcctt tatcagcttt
      241 gctgtacctg caggtacttc ctatcagata acatcctatc caacagaaaa tacatcatgt
      301 ggtgccgggg tattttcagt atttggatat caaacttaat tgagcgttac acacgacgca
      361 atacaacctg tcttatgctc gtatttgtac catttgcgtg aacctgctgg gctctcaact
      421 acctggcagt agtgggcatc ctctgcgttg cccattctgg caatggcaca gaagtcgaat
      481 tgccccagat aacctgtggt ggaaagctga gtaactttga gttttccacc tattgtcc
//
LOCUS       DAZGCS010000457          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGCS010000457 DAZGCS010000000
VERSION     DAZGCS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.465"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..480)
                     /locus_tag="ACHK01_005575"
     CDS             complement(<1..480)
                     /locus_tag="ACHK01_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO8100272.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAI
                     "
BASE COUNT          151 a           98 c           82 g          200 t
ORIGIN      
        1 aatggccata atagtactat taactctcaa ttttatattt ttatcatcac tacaaatatt
       61 aactgaattt atatcaatga tttttttatt cttatctttt attttattta ctgtttcaat
      121 gcaatcagcg gcaagttgga gtggatttag atttttcaga tatggtctta tgatctttcc
      181 cttacttaat aaatagcatg tataggattc ggaaagtcca gcatctattt tgtacctcat
      241 tatattagat aaggatgtga gaaattcctt gttatcattc gcagccatat atgccagtcc
      301 gagttcagcc tctgcggcct tacaggcaaa atagcttagt gaacacgctt ttcgtccctc
      361 tttagtactg gctgatatta gtgcccataa tattgctttg tctgccaaat ctaactctct
      421 ctgaaattgt cttgcagtaa ctgcactcgc agaaatagag aaaaaaatac aattgttaat
      481 aaccttgcca tattttttag tccgtaactg ttaatgtgct atctggctct g
//
LOCUS       DAZGCS010000458          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGCS010000458 DAZGCS010000000
VERSION     DAZGCS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.466"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>514)
                     /gene="espL2"
                     /locus_tag="ACHK01_005576"
     CDS             complement(<1..>514)
                     /gene="espL2"
                     /locus_tag="ACHK01_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO8100273.1"
                     /translation="LIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNLVLDTIDKNI
                     DLEYLLSAFKYEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEA
                     RNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISH
                     RHDHVVKLYGK"
BASE COUNT          170 a          100 c           71 g          173 t
ORIGIN      
        1 ctttccgtat aatttgacaa catgatcatg tctatgactg atcgctaatg ataaccccgt
       61 aaaatcataa tgttttgcag tcaataactc ttcgataaaa tcctttgata tatctaacat
      121 tattagtgag tcaaaaaaag actgaatcgt attaatatct ccattttgat acgcaagaag
      181 taatcctggt gcaccatcat aatttctcgc ctcaagcatc ctgattatct cacctctttt
      241 caaattacta ttgccaagta cgccacaata tgcctttatt gcgtctgcat gaccattttg
      301 aaatgcagaa aataatccag gagtatattt attagagcta tgtgcctcat atttaaatgc
      361 tgaaagcaaa tactctaaat ctatattttt atcaatagta tccagaacta agttaccata
      421 actcttaata gcttgagcat gtccattttg aagggcctga tataaaccag gtatggttcc
      481 atccaaagtt cttgcagata atatatgtat taat
//
LOCUS       DAZGCS010000459          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGCS010000459 DAZGCS010000000
VERSION     DAZGCS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.467"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..279)
                     /locus_tag="ACHK01_005577"
     CDS             complement(1..279)
                     /locus_tag="ACHK01_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100274.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(447..>506)
                     /locus_tag="ACHK01_005578"
     CDS             complement(447..>506)
                     /locus_tag="ACHK01_005578"
                     /inference="COORDINATES: protein motif:HMM:NF013968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8100275.1"
                     /translation="RVRTGQTEVAVFTAYEPEE"
BASE COUNT          107 a          125 c          157 g          117 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgcg ggacggacgc ggtggccgtt cggagtaagg
       61 gagcctgacg gagattatcc agtgacggta gtcgaggcta agggcttttt taacctcgca
      121 tccgcgcctg cggtaacact gaatgagcca ttcggcctgt tcttcagtgc atgggggatg
      181 ctggtaccag tcagatttga atgcgtgaaa acaccgtccg cgcctgctgg caaagacggc
      241 agaatcatca gaattgtata atttggtatc gtgcgccatc ggttgtctct gctggcgcag
      301 caggtgccag ttgttcaggc tggcgtgcga attgtaaacc agaatgccag gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgattcat cagaggtggc
      421 gagggatttc tcccccgcca ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gactcg
//
LOCUS       DAZGCS010000460          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGCS010000460 DAZGCS010000000
VERSION     DAZGCS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.468"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>503
                     /locus_tag="ACHK01_005579"
     CDS             <1..>503
                     /locus_tag="ACHK01_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100276.1"
                     /translation="TANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRR
                     KVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYTIQYVQ
                     NVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCLDDENMSIQPEQ
                     INTPGIS"
BASE COUNT          180 a           65 c          102 g          156 t
ORIGIN      
        1 cactgcaaat aattatttga aacaggcctg gaacgatata aaaaatgagt acgaaaaaaa
       61 tcaaacatat tcaatcacgc tttttgaaaa cacactggtg tgttttatgc ggttatacaa
      121 tgaactcaga cgtaaagtaa atgaagagga tactccatgt ctggaatgtg aatcactaga
      181 aaaagaattt gaggaaatgc agaatgataa tgatctatca ttatttatga gaacattgcg
      241 tactaatgat acacaaattt attcaggggt ttcaggaggt attacatata ctatacaata
      301 tgttcaaaat gttgatattg ttagagtgtc cttgccgggc agaggttcag agtctatcac
      361 agattttaaa ggttattatt ggtatggctt tatggaatat attgaaaaca ttactgcgtg
      421 tgatgatgtt ttttctgagt attgtttaga tgatgaaaat atgagtatcc agccagaaca
      481 gataaatacg ccgggaatat ctg
//
LOCUS       DAZGCS010000461          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGCS010000461 DAZGCS010000000
VERSION     DAZGCS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.469"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..265
                     /locus_tag="ACHK01_005580"
     CDS             83..265
                     /locus_tag="ACHK01_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100277.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            278..409
                     /locus_tag="ACHK01_005581"
     CDS             278..409
                     /locus_tag="ACHK01_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100278.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            417..>499
                     /locus_tag="ACHK01_005582"
     CDS             417..>499
                     /locus_tag="ACHK01_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8100279.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYR"
BASE COUNT          112 a           91 c          147 g          149 t
ORIGIN      
        1 ctaagtgaat aaaagtaaaa ccccgtttgt tggccgcaaa cggggttttg tgtttctgac
       61 cttgagtaag gcaagggaga acatgggaaa gtataaacga attctgttga gattgactat
      121 gaaaaacggc cttgaactga aagcgcctgt aactgatgac gtcagcagag cgctggcttt
      181 tgctattaag tgggtggcgg tcggtattgc tgtgtctccg atgctgtatg ggctggcaaa
      241 actggtcatt gcgttgaaat cgtgaagagg attaagcatg tcagacaagc tcataacgct
      301 ggcgaagatc ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg ttgctatttg
      361 cttttccact gcctggcgcg tcttaacttt gtcatggctg gtggggtgag ggggatatga
      421 accgtgttct gtgtgtggtg attattgtcc tgctggtagc ctgtggtgtg cttagtctgg
      481 ggctgaatca ttaccgcga
//
LOCUS       DAZGCS010000462          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGCS010000462 DAZGCS010000000
VERSION     DAZGCS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.470"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          159 a          107 c           91 g          140 t
ORIGIN      
        1 gaaataatga tctggcagcc tgagttcaca gataaaatac tctccaggaa acccggggcg
       61 gttcacccct aattcaacta attggaggca ttaccatgca ttagtgcaag cgccttgtgg
      121 attcttttgc taatgacgga aacatattgt acaatattgc atttatccct caataaataa
      181 atgagttatc agattgcaaa aatctgttat caaactaatt caaataaaaa gagattgata
      241 cgaccaacgt acacaaaaat tttcgatatt cgaactaaat aacagctcct aataccgcta
      301 cactttagct atccagtctg cgcctcagaa atggacgatg ccgttgaagg actggtgtat
      361 ggcaatgagc cgctttatta tcgaattcgg tgaccgcctg gacggtcact tcggagaaaa
      421 ggcatttaca cagcatccta aacaggttca gcataggtga tataccgcct tttcattttc
      481 ttctccatta tttataa
//
LOCUS       DAZGCS010000463          495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGCS010000463 DAZGCS010000000
VERSION     DAZGCS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.471"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..243
                     /locus_tag="ACHK01_005583"
     CDS             <1..243
                     /locus_tag="ACHK01_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100280.1"
                     /translation="WKSSSASIWTNIKTFTLYPKNTQVLGRFKLCINTYRIDGREMAE
                     TEVVPIDMPDSNGEMTWQAKNYTQYSSYFMKITCLK"
BASE COUNT          141 a          111 c          109 g          134 t
ORIGIN      
        1 tggaaatcat catcagcgtc gatatggacg aatataaaaa catttacgtt atatcccaaa
       61 aacacacagg tcttagggag gtttaaactg tgtattaaca cctatcgtat tgacgggcgt
      121 gaaatggcag aaacagaagt cgttcctatt gacatgcctg actctaacgg cgagatgact
      181 tggcaagcca aaaactacac acagtattcg tcctacttta tgaagattac ttgcttgaaa
      241 tagcttattg gcaaatggca tatacagtta ttgaaccatt ctgatcttcg gttgtatcgc
      301 acgcagccga ccatgagtta ttggctgatg ggaatgacct ggtaagccat atgtaaccag
      361 ttcgtgaggt gcatattccg ccgccacctg taacacgttt tccagttgga caagtagctg
      421 ttgctccagg aaagcgccaa cctgtggcct gtgctgtagc aatttgaagc tcaccagatc
      481 ctacctttct ccagg
//
LOCUS       DAZGCS010000464          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGCS010000464 DAZGCS010000000
VERSION     DAZGCS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.472"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..370
                     /locus_tag="ACHK01_005584"
     CDS             74..370
                     /locus_tag="ACHK01_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO8100281.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIGHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            376..>485
                     /locus_tag="ACHK01_005585"
     CDS             376..>485
                     /locus_tag="ACHK01_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO8100282.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          125 a          124 c          127 g          109 t
ORIGIN      
        1 cccctttcct gttttcctaa tcagcccggc atttcgcggg cgatattttc acagctattt
       61 caggagttcg gccatgaacg cttattacat tcaggatcgt cttgaggctc agagctgggc
      121 gcgtcactac cagcagatag cccgtgaaga gaaagaggca gaactggcag acgacatgga
      181 aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc ggtcatttgc aacgccacgg
      241 ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat
      301 ggcagaacac atccggtaca tggttgaaac cattgctcac catcaggttg atattgattc
      361 agaggtataa aacggatgag tacagcactc gcaacgctgg cagggaagct ggctgaacgt
      421 gtcggcatgg attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt
      481 aaagg
//
LOCUS       DAZGCS010000465          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGCS010000465 DAZGCS010000000
VERSION     DAZGCS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.473"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..485)
                     /locus_tag="ACHK01_005586"
     CDS             complement(<1..485)
                     /locus_tag="ACHK01_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8100283.1"
                     /translation="MRVFHGGRVLVESEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     NERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVS
                     RR"
BASE COUNT          119 a          140 c          120 g          106 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcat
//
LOCUS       DAZGCS010000466          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGCS010000466 DAZGCS010000000
VERSION     DAZGCS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.474"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..267)
                     /locus_tag="ACHK01_005587"
     CDS             complement(<1..267)
                     /locus_tag="ACHK01_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO8100284.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNEPSKIDKMLEINSNRDLLGMFFLSLKEDSVLLNIA"
BASE COUNT          168 a           67 c           90 g          160 t
ORIGIN      
        1 tgcaatattc aaaagtacgc tatcttcttt taatgataga aaaaacatac cgagtagatc
       61 tcgattgcta tttatttcta gcattttatc aatttttgaa ggttcattat tgaataaata
      121 acaataaaaa aacaggacct cagattgagg cgcacactcc agtatccatt catgaccatc
      181 gtgatcatgg aaagagaggg cgttagtttg ttcttcaaga gttattccga acgtatgtgc
      241 aagatctgtg cgaaattgtt cattcatatt gtagtcctgt cattctttca atgagggtat
      301 gcttaaacgt aattaactcg tgagttctgg cacaaataat aagacgaaaa tataagatgc
      361 gagtaatagt gttgcatatt tatgatagca tgtgataaat gtagtgcaac tacttgtgga
      421 tagtgccgga ggaaaattta aatataaaac aattattgtt tttaatgaaa atgaaaaata
      481 ttttg
//
LOCUS       DAZGCS010000467          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGCS010000467 DAZGCS010000000
VERSION     DAZGCS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.475"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            240..>481
                     /locus_tag="ACHK01_005588"
     CDS             240..>481
                     /locus_tag="ACHK01_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8100285.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          157 a           97 c           81 g          146 t
ORIGIN      
        1 tttttacgtg ataattcagg agacacagaa tgcgcataaa aataacagca taaaacacct
       61 taccaccacc caagaatttc atattgcatt gtttttcaat gaaaaaatat tattcgcgta
      121 atatctcacg ataaataaca ttaggatttt gttatttaaa cacgagtcct ttgcacttgc
      181 ttactttatc gataaatcct acttttttaa tgcgatccaa tcattttaag gagtttaaaa
      241 tggataagaa gcaagtaacg gatttaaggt cggaactact cgattcacgt tttggtgcga
      301 agtctatttc cactatcgca gaatcaaaac gttttccgct gcacgaaatg cgcgacgatg
      361 tcgcattcca gattatcaat gacgaattat atcttgatgg caacgctcgt cagaacctgg
      421 ccactttctg ccagacctgg gacgacgaaa atgtccacaa attgatggat ttatccatta
      481 a
//
LOCUS       DAZGCS010000468          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGCS010000468 DAZGCS010000000
VERSION     DAZGCS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.476"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>480
                     /locus_tag="ACHK01_005589"
     CDS             <1..>480
                     /locus_tag="ACHK01_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFO8100286.1"
                     /translation="LIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQKR
                     FLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDL
                     QGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVV
                     "
BASE COUNT           88 a          156 c          157 g           79 t
ORIGIN      
        1 ctgatccggc gtgcccggca aggcagcggc ctcgtgacgg atctggtgcc cggccagtcc
       61 ggtggaggta aaggtaaggg gcgcttgccg gaaccggtcg agcgcgtcat ccacgagcta
      121 ctgcaaaagc ggttcctgac caagcagaag cgcagcctag cggcctttca ccgcgaagtc
      181 actcaggtgt gcaaggctca aaaactgcga gtgccggcgc gcaataccgt ggccttacgg
      241 atcgctagcc ttgacccgcg caaggtcatc cgccggcggg aaggccagga tgccgctcgt
      301 gacctacaag gtgtgggcgg cgagcctcct gccgtgaccg cgccgctgga gcaggtgcag
      361 atagaccata cggtcatcga cctgatcgtg gtcgatgacc gcgaccggca acctattggc
      421 cgcccgtacc tgaccctcgc catcgacgtg ttcacccgct gcgtgctcgg catggtcgtc
//
LOCUS       DAZGCS010000469          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGCS010000469 DAZGCS010000000
VERSION     DAZGCS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.477"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>471
                     /locus_tag="ACHK01_005590"
     CDS             <1..>471
                     /locus_tag="ACHK01_005590"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8100287.1"
                     /translation="IAKPRSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQY
                     ENDARALFEFTSGVNVTESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKF
                     RLGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMA"
BASE COUNT          116 a          117 c          131 g          107 t
ORIGIN      
        1 atagcaaaac cccgctccgg aaaaaagtgg cctgacatga aaatgtccta cttccacacc
       61 ctgcttgctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc gctggcctgg
      121 ggaaaacagt acgagaacga cgccagagcc ctgtttgagt tcacttccgg cgtgaatgtt
      181 actgaatcac cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc tccagatggt
      241 ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg ggatttcatg
      301 aagttccggc tcggtggttt cgaggccata aagtcagctt acatggccca ggtgcagtac
      361 agcatgtggg tgacgcgaaa agatgcctgg tactttgcca actatgaccc gcgtatgaag
      421 cgtgaaggac tgcattatgt cgtggttgag cgggatgaaa agtacatggc g
//
LOCUS       DAZGCS010000470          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGCS010000470 DAZGCS010000000
VERSION     DAZGCS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.478"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>471)
                     /locus_tag="ACHK01_005591"
     rRNA            complement(<1..>471)
                     /locus_tag="ACHK01_005591"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          152 c          100 g          132 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcgcacttct gatacctcca g
//
LOCUS       DAZGCS010000471          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGCS010000471 DAZGCS010000000
VERSION     DAZGCS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.479"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>470
                     /locus_tag="ACHK01_005592"
     CDS             <1..>470
                     /locus_tag="ACHK01_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100288.1"
                     /translation="FTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQPINS
                     IFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETISSTPL
                     TQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV"
BASE COUNT          187 a          102 c           69 g          112 t
ORIGIN      
        1 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
       61 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
      121 acccattaat tccatattct ctggagaagg atacaaccct gaacaatttg atttagcgca
      181 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
      241 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
      301 accacttacg caacagacac aatcaatact taatcagatt aataacatta gatatgaaaa
      361 aaacaaaagt gctgaatgtc gaatcattgt tgtcgctaat cctaaacccg acaaggcaat
      421 cataactaaa attagcgtag aagagggaat acccataaca ttcagtgtcc
//
LOCUS       DAZGCS010000472          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGCS010000472 DAZGCS010000000
VERSION     DAZGCS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.480"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>470
                     /locus_tag="ACHK01_005593"
     CDS             <1..>470
                     /locus_tag="ACHK01_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8100289.1"
                     /translation="GWRGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAW
                     DILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNL
                     FGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTI"
BASE COUNT          159 a           65 c          119 g          127 t
ORIGIN      
        1 gggctggagg gggggatgga gtctgtatgc gtacccgctg aatccggtga atggtattga
       61 tccattaggg ttaagtcccg cagatgtagc gctaataaga agaaaagatc aactaaacca
      121 tcaaagagca tgggatatat tatctgatac ttatgaagat atgaagagat taaatttagg
      181 tgggactgat caatttttcc attgtatggc attttgtcga gtgtctaaat taaatgacgc
      241 tggtgttagc cgatcggcga aagggctggg ttatgaaaaa gagattagag attacgggtt
      301 aaatctgttc ggtatgtacg gcagaaaagt aaagctatcc cattctgaaa tgattgaaga
      361 taataaaaaa gacttggctg taaatgacca tgggttgaca tgtccatcaa caacagattg
      421 ctcagataga tgtagtgatt atattaatcc agagcataaa aaaacgataa
//
LOCUS       DAZGCS010000473          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGCS010000473 DAZGCS010000000
VERSION     DAZGCS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.481"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(207..>464)
                     /locus_tag="ACHK01_005594"
     CDS             complement(207..>464)
                     /locus_tag="ACHK01_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO8100290.1"
                     /translation="QKMTQESGCLSRLFSFLSGGVTKALESKLNERHITPLSPTWFPR
                     TPLEGVLTERGLSSLLRCVQSTENLDYSNAMISNSFSNSVV"
BASE COUNT          161 a          103 c           76 g          124 t
ORIGIN      
        1 tgataaatta tgagctggaa ggaattcatc cattcagctc aatcccctcc tcatcacaga
       61 atctctgact tctgccagcc aaactcactc tgaatattca tcccatattt tcctaaaaac
      121 aatgtattat cgacataaaa caaacagaaa aactccagaa tcacaattat tctggagtta
      181 aaccaggagg aaagctcaaa tgataatcac actacagagt tagagaaaga gtttgatatc
      241 atagcattac tataatcaag gttttcagta gactgtacgc aacgtaataa agaagataat
      301 cctctttccg ttaagacccc ctccagtgga gttcgaggaa accaggttgg agaaagtgga
      361 gtgatatgcc tctcatttag tttactttcc aaagccttag tcacaccgcc agaaagaaag
      421 ctaaataatc ttgagagaca cccactttcc tgagtcatct tttg
//
LOCUS       DAZGCS010000474          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGCS010000474 DAZGCS010000000
VERSION     DAZGCS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.482"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..>464)
                     /locus_tag="ACHK01_005595"
     CDS             complement(123..>464)
                     /locus_tag="ACHK01_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100291.1"
                     /translation="LNINVGLGSDIPAGHSPSIFEACLHAITASKALNDGGNSQLSSE
                     VWGYSGASVSFREAFWLATGGDGKILDLPIGKLRKDYFVDTIVIDTNGHNLDIIIYDD
                     TTEDILQKLII"
BASE COUNT          138 a          105 c           70 g          151 t
ORIGIN      
        1 tttcgctaaa aacattgtta ttgataaact gtcagagaag tatttctacc cttctctgac
       61 tacctctttg ataaatattc tcaccattaa cacatacctt gcatatatta ttacgctgcg
      121 tgttatatta ttaacttctg cagaatgtcc tcagttgtat catcatatat tatgatatct
      181 aaattatgac cattagtatc aattactata gtatcaacaa aataatcttt ccgaagtttt
      241 cctattggta aatccagaat ttttccatca ccgcctgttg caagccagaa agcctccctg
      301 aatgaaacag atgctccgga gtatccccag acctcagaag ataactgaga gttgccccca
      361 tcgttaaggg ctttacttgc tgttattgca tggagacacg cctcaaatat cgatgggcta
      421 tgtcctgctg ggatatcact cccaagacca acatttatat tcag
//
LOCUS       DAZGCS010000475          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGCS010000475 DAZGCS010000000
VERSION     DAZGCS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.483"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..300)
                     /gene="yrhD"
                     /locus_tag="ACHK01_005596"
     CDS             complement(145..300)
                     /gene="yrhD"
                     /locus_tag="ACHK01_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO8100292.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT          151 a           70 c           77 g          164 t
ORIGIN      
        1 gagcaaagac gcgttcatct tcgtcaaaga tattattaaa tgctggtttt gaaaaaagtt
       61 catcggataa gatatagtca cgaaataagt atctgctaat attttatgat ttctttttca
      121 gaacgtcgaa cgggattttc ttatttaaga tatcagaatc ttatgattcg ggtaaaattc
      181 taaataaatc aacatgtcat taaaatcatc atagccatta gctatttcgg ctaaaataga
      241 gactacatgt cttcggtcca tctcacttaa ggagtgtagt tccgttgtaa gtttttccat
      301 agcttgcact gctaaatttc gaacaaggaa ttttctgctg gtaatctcta aaaagatggc
      361 atggtttaca atgatttttg tttccttttg attattatga acaactgtcc atgatttcgt
      421 ttaagaatga agagaaatca ctaaacgaac tgaatatatt tt
//
LOCUS       DAZGCS010000476          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGCS010000476 DAZGCS010000000
VERSION     DAZGCS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.484"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..455)
                     /locus_tag="ACHK01_005597"
     CDS             complement(<1..455)
                     /locus_tag="ACHK01_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8100293.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSS"
BASE COUNT          125 a          115 c          101 g          118 t
ORIGIN      
        1 gatgaaaagc gtttcagcgt gagagtattg tcacgatgtt tacgacccag ccatggggaa
       61 aggtctttca ggcatggcac gttccgaatc gtcgcctcca cgtgagactt cataaaatct
      121 tcagcggcag aatccgtggg ctgaaaaagc agactgtttc gggatttatg ctcaataaaa
      181 tacccgacca cccccagcaa catctttgta tagccaacac gggcagattt aatcaggtta
      241 accgtgcgaa cctggtcgtt acccatacag ttcataatgg cgatctggaa tggcagcgtt
      301 ttccattctc cctcaccata tgaagattct ttaggcagat aataattttg atcagcccat
      361 tcaactgccg tcattggtac aaccctgacc agaggctgca gcgcaaccga aacggcagcc
      421 atcatattat tcagttgttg ctctgatata ttcatcgag
//
LOCUS       DAZGCS010000477          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGCS010000477 DAZGCS010000000
VERSION     DAZGCS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.485"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>455)
                     /locus_tag="ACHK01_005598"
     CDS             complement(<1..>455)
                     /locus_tag="ACHK01_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8100294.1"
                     /translation="SEHQSTLSAGYLHASTNVPGSDDLNGINVKYRYEFTDTLGMVTS
                     FSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVST
                     FSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPIESVAID"
BASE COUNT          104 a          139 c          111 g          101 t
ORIGIN      
        1 atatcaatgg ccacggattc gatcgggtta aactgcacgc cagctcccca cgccagagac
       61 gtgttgctgt ggcgaccgtc atcacttccg gtcagcacat catgcgtttt ccccttgttg
      121 tcagttacgc ggagataatc cccggaaaaa gtcgacacac ggctgtaagc cacgcccgcc
      181 atcgcatacg cactgaacca ttcattcacg cgcacagagg gccccgccat cacgctgaac
      241 cagcggttac gaacggaatc ttcatgccag cgggtatcgc tgtaacgggt aatctggcga
      301 ttcttgtctc ctgcatagct gaatgacgtc accatcccca gtgtgtccgt aaactcataa
      361 cggtatttca cgttaatccc gttcagatca tcgctgccgg gaacgttcgt cgaggcatga
      421 agataccccg cgctcagcgt ggactgatgt tcaga
//
LOCUS       DAZGCS010000478          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGCS010000478 DAZGCS010000000
VERSION     DAZGCS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.486"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..409
                     /gene="espX1"
                     /locus_tag="ACHK01_005599"
     CDS             <1..409
                     /gene="espX1"
                     /locus_tag="ACHK01_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO8100295.1"
                     /translation="LCFESDFSNGEINNLFIKKSNIFGASFTNTRIKNLLCVDIMPGR
                     WTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNK
                     FMHDYDKTSSEMRKYLKSINECMFLMKNIAHQN"
BASE COUNT          180 a           73 c           69 g          128 t
ORIGIN      
        1 attatgtttt gaaagcgact tttcaaatgg agaaattaat aatctcttca ttaaaaaaag
       61 caacatattt ggagcatcat tcactaatac cagaataaaa aatttgctct gtgtagatat
      121 tatgcctggt cgatggacca ctcaacttgt caataagcac ttaggttaca gatacacagg
      181 agtattcaaa accctggcaa gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc
      241 attggtacaa actttagttc gcgacaatgt aaagctaaat aatgatgtgt ataaagaact
      301 taataagttt atgcacgatt acgacaaaac atcatcagaa atgagaaaat accttaaaag
      361 catcaacgag tgtatgttcc ttatgaaaaa catagctcat caaaattaaa aaatctcaaa
      421 aaagaagtaa tttcagaatg aaattaactg
//
LOCUS       DAZGCS010000479          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGCS010000479 DAZGCS010000000
VERSION     DAZGCS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.487"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..>448
                     /locus_tag="ACHK01_005600"
     CDS             106..>448
                     /locus_tag="ACHK01_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8100296.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTKPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLIHDEID
                     ILRLCVSYSLKQ"
BASE COUNT          149 a           87 c           63 g          149 t
ORIGIN      
        1 gtataaaccc tattccctca atgttaagca aatataacaa tcactcttat tttaaatttt
       61 acacgtaata tttaccctaa atgattttag agtacaggtg ggcatatgca agtcttttcc
      121 agtgatgtct acttcactgt aggcactaat gcattattag catcccaaaa agaatattac
      181 agcgatttag ttgcgttggt tgatcttgga cattcgttcg ttgttattga tgagcatcaa
      241 catcgaaatc tcaaaccaaa tacaaaacct gtaaacatcc ttttgagcaa caatttcatc
      301 aggatcaata agaatattac attatctgat ttaacgcatt tcctggtttc aaatcttcat
      361 acacaaaatg tgtattccac acaagaacca cttatccatg atgagataga tatcctgcgg
      421 ttatgtgttt cttactcgtt aaaacaaa
//
LOCUS       DAZGCS010000480          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGCS010000480 DAZGCS010000000
VERSION     DAZGCS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.488"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>441)
                     /locus_tag="ACHK01_005601"
     CDS             complement(<1..>441)
                     /locus_tag="ACHK01_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8100297.1"
                     /translation="VTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA
                     EDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSSLTAMTASF
                     VLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFD"
BASE COUNT           88 a          167 c          109 g           77 t
ORIGIN      
        1 tgtcgaactc atccgccacc gcagggccgt tatacccgca ttcatctccc cggtaatccc
       61 acatacaggt gttcgccagc atgatgcgac cgggaaacag cgccccgtcc gtctcggtcg
      121 gtgtggccag cacaaacgag gccgtcatgg ccgtcagcga tgacatctgc tccaccaccc
      181 agcggtctct cagctcctgc tccgggtccg cttccggatt gcctgccaca aagttcaccg
      241 catccagaaa acgggcatac acccggcggc ggaccaccgt ggcacccacc aggctctgca
      301 aatcctccgc catcccggtg acaagaccga acagattgga caccgtcagc gacgggcggg
      361 cactgctgcc ctttccgttc atctcaaagc cactcccctc aatcgggtac gcctgatatt
      421 cacgcccctg ccaggtgacc g
//
LOCUS       DAZGCS010000481          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGCS010000481 DAZGCS010000000
VERSION     DAZGCS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.489"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            145..369
                     /locus_tag="ACHK01_005602"
     CDS             145..369
                     /locus_tag="ACHK01_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8100298.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQSTCSDRFT"
BASE COUNT          167 a           96 c           69 g          103 t
ORIGIN      
        1 catgtcacaa cgtgcatcta cgccatcaaa ccacgagaaa taatcaatta ttacgcaggt
       61 atcgtattaa ttgatctgca tcaaattagc gtaaaagcaa cttcagataa tacaaatcag
      121 caacactgaa tacggggcaa cattatgtca tcaaagaaca gaacccgcag aacaacaacc
      181 cgcaacatcc gatttccaaa ccagataatt gaacaaatta acatcgctct tgacctgaaa
      241 ggttcaggta atttttcagc gtgggttatt gaagcctgca gaagaagatt aattaatgaa
      301 aaatattctc aatttgtacc caacaaagac aaacacgacc agagcacctg ttcagacagg
      361 tttacttaaa cgacttatat atgacacaaa aagcgaccac taaagtcgct ttttcttatg
      421 gtaacaggca ataac
//
LOCUS       DAZGCS010000482          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGCS010000482 DAZGCS010000000
VERSION     DAZGCS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.490"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>435)
                     /locus_tag="ACHK01_005603"
     CDS             complement(<1..>435)
                     /locus_tag="ACHK01_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO8100299.1"
                     /translation="LGDLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELSD
                     INKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQ
                     GGQCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLP"
BASE COUNT          139 a           90 c           61 g          145 t
ORIGIN      
        1 cggtaaaaga tatttgacta tagtccataa gggattatca gaaaatagcc attcaagatt
       61 tcctgcaccg tattttatca gttcagaaca aaaccgttct tcgccatatt ctggtttaca
      121 ttgccctccc tgccaataga atgataactg cttcacattt tgatatccta tctctctgat
      181 cagagtttct cgatataatc gtgaaaaaat atagtcaggg cattgaattt caaataatgc
      241 actacttaga gcaagcaatc ttattttccc aattgactga tataaaaaac cttccttatt
      301 tatatcagat aattccactc cagaaatttt atcttgtatt tcctgacgca atatcttgtt
      361 gagttgatta cgagtagaac tactttctct taaatgccga aaactaacct gattgacacc
      421 ggttaaatca cctag
//
LOCUS       DAZGCS010000483          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGCS010000483 DAZGCS010000000
VERSION     DAZGCS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.491"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..>434
                     /locus_tag="ACHK01_005604"
     CDS             183..>434
                     /locus_tag="ACHK01_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8100300.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVIN"
BASE COUNT          107 a          106 c          107 g          114 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taat
//
LOCUS       DAZGCS010000484          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGCS010000484 DAZGCS010000000
VERSION     DAZGCS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.492"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..348
                     /locus_tag="ACHK01_005605"
                     /pseudo
     CDS             1..348
                     /locus_tag="ACHK01_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            345..>433
                     /locus_tag="ACHK01_005606"
     CDS             345..>433
                     /locus_tag="ACHK01_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO8100301.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVL"
BASE COUNT           99 a           90 c          142 g          102 t
ORIGIN      
        1 attaacgccg catacctggt ggcctgtgca aatggtgaat gtgaggcttc ctagaaagcg
       61 aagatcgaac aggtactgcg taatcagcca gcgctgtccg cgtttacgtc agaaattaat
      121 gcgattagcg caaccattat cggtcagctg gatacgaact ttaaaattgg tcgtcgtgcg
      181 gcgttacgtg agatcgagga tgtgaaacgc gatacgcgtg aagcggaaga tgtgctggat
      241 gtggcggtgg ccattgcgga ggcagacggc gaaattgagc cggaagagcg caaggtgctg
      301 gaagagattg ccggtgttct ggggcttcgt ctggagaatc acctgtgacg gtaaaactgc
      361 gcctggctgt ggctgcactc ctgctgtttc tggtggtgat ggtggatttc accagcagaa
      421 tcatgtcggt gct
//
LOCUS       DAZGCS010000485          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGCS010000485 DAZGCS010000000
VERSION     DAZGCS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.493"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>432
                     /locus_tag="ACHK01_005607"
     CDS             <1..>432
                     /locus_tag="ACHK01_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8100302.1"
                     /translation="DGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDA
                     TGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLI
                     AVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI"
BASE COUNT          108 a          123 c          127 g           74 t
ORIGIN      
        1 gacgggctgg aaatgcgccg caaatatgat gagtacggac gtctgattca ggaaaccgcc
       61 cctgacggcg atatcacccg ctaccgttat gataatccac acagtgacct cccctgcgca
      121 acggacgatg ccaccggcag ccggaaaacc atgacgtgga gccgttacgg tcagttgctg
      181 agcttcactg actgttccgg ttatgtaacc cgctatgacc atgaccgttt tggtcaggtg
      241 acggcggtgc accgcgagga agggctgagt cagtaccgcg catacgacag ccgtggacag
      301 ttaattgccg tgaaagacac gcagggccat gaaacgcggt atgaatacaa cgccgccggt
      361 gacctgacca ccgtcattgc cccggacggc agcagaaacg ggacacagta cgatgcgtgg
      421 ggaaaagcca tc
//
LOCUS       DAZGCS010000486          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGCS010000486 DAZGCS010000000
VERSION     DAZGCS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.494"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>432
                     /locus_tag="ACHK01_005608"
     CDS             <1..>432
                     /locus_tag="ACHK01_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8100303.1"
                     /translation="DGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDA
                     TGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLT
                     AVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAI"
BASE COUNT          107 a          122 c          127 g           76 t
ORIGIN      
        1 gacgggctgg aattgcgccg ggaatatgat gaatccggcc gtctgattca ggaaactgcc
       61 cctgacggcg atatcacccg ctaccgttat gataatccac acagtgactt accctgcgca
      121 acggaagatg ccaccggcag ccggaaaacc atgacgtgga gccgttacgg tcagttgctg
      181 accttcactg actgttccgg ttatgtaacc cgctatgacc atgaccgttt tggtcaggtg
      241 acggcggtgc accgcgagga aggcctgagc cagtaccacg catacgacag ccgtggacag
      301 ttaactgcgg tgaaagacac gcagggccat gaaacgcggt atgaatacaa catcgccggt
      361 gacctgaccg ccgtcattgc cccggacggc agcagaaacg ggacacagta cgatgcgtgg
      421 ggaaaggcca tc
//
LOCUS       DAZGCS010000487          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGCS010000487 DAZGCS010000000
VERSION     DAZGCS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.495"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..>430
                     /locus_tag="ACHK01_005609"
     CDS             19..>430
                     /locus_tag="ACHK01_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO8100304.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTK"
BASE COUNT          147 a           62 c           87 g          134 t
ORIGIN      
        1 aattagcggg tggtattcat ggaaaataat gataaattct tatcacaaga cttattggaa
       61 tcttatgcca ttcgtttgtt gagcggacca ttgaatggat gcgagtatga aatacttaat
      121 gggcgtctac ttgttatcat tggtaatgat gtttcgttag gaagaagtga tgctttttct
      181 gagttaccag aaaacacaat agtcgttcct tatggcgagc ttacaggtag ttttgagata
      241 ataattacta ccgaccctga tctggtagta acattcagag aattaacagc acaagaacct
      301 gaagatagaa cgttaacatt gaatcagcaa atagaagttt taggtctcaa atttgctgta
      361 aaagaaaaaa atgaagtttg gcagtattca ttgccaggca ttattgaaaa taacattatt
      421 tctacaaaac
//
LOCUS       DAZGCS010000488          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGCS010000488 DAZGCS010000000
VERSION     DAZGCS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.496"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..160)
                     /locus_tag="ACHK01_005610"
     CDS             complement(<1..160)
                     /locus_tag="ACHK01_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100305.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYAL"
BASE COUNT          143 a           89 c           66 g          127 t
ORIGIN      
        1 ttaatgcata atcagatgcc gcctcatcat tatcctcagt attatcatag ccgaaaactt
       61 ctcccggaca cgggcgttca cgataatagt catcgtcata gttttcatcg tcagataatg
      121 atatgtcgta ttctgaatat gattcaccac aattatacat gaaatcatct cctttggggg
      181 gggcaataat tagtgagttt attcgaaaaa atgatgagcg ccagaccaat ccacccaact
      241 tttgattata atcatgaaga tacttgtctc agtacaaatt gaatcaaact caatgcagca
      301 tgattcacct ttatattctc tcagggaaac caaataccac tactcaacta ttaagaacaa
      361 aattcgggat gtatgattat cagaagatac ctgcatccta aataatttca gacagatatt
      421 atcgt
//
LOCUS       DAZGCS010000489          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGCS010000489 DAZGCS010000000
VERSION     DAZGCS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.497"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>413
                     /locus_tag="ACHK01_005611"
     CDS             <1..>413
                     /locus_tag="ACHK01_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8100306.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRS
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRD"
BASE COUNT           76 a          126 c          127 g           84 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggtctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg tgccgtccgt gac
//
LOCUS       DAZGCS010000490          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGCS010000490 DAZGCS010000000
VERSION     DAZGCS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.498"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..196)
                     /locus_tag="ACHK01_005612"
     CDS             complement(<1..196)
                     /locus_tag="ACHK01_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8100307.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSG"
     gene            complement(196..>403)
                     /locus_tag="ACHK01_005613"
     CDS             complement(196..>403)
                     /locus_tag="ACHK01_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100308.1"
                     /translation="KTYSVTLSVPREEATVLELFLEEHGGWKSFLWTPPYEWRQIKVT
                     CAKWSSRVSMLRVEFSAEFEQVVN"
BASE COUNT           86 a          137 c           87 g           93 t
ORIGIN      
        1 aaccgctccc ctgaatggga tacggctgat actgtcgccc ctgccaggtg accggctcac
       61 ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc accgacctct gtcaggtcga
      121 tttcccagag caccacgctg gccgactgct ccgcacgggt gcattcattc agtgtttcct
      181 gccggatatc ctgcatcagt tcaccacctg ttcaaactct gcgctgaact caacacgcag
      241 catactgacc cgcgacgacc attttgcgca ggtcaccttt atctgccgcc actcataagg
      301 cggcgtccac agaaaggatt tccagccccc gtgctcttcc agaaacaact ccagtaccgt
      361 ggcctcctca cgggggacag aaagcgtcac gctgtacgtt ttc
//
LOCUS       DAZGCS010000491          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGCS010000491 DAZGCS010000000
VERSION     DAZGCS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.499"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>402)
                     /locus_tag="ACHK01_005614"
     CDS             complement(<1..>402)
                     /locus_tag="ACHK01_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8100309.1"
                     /translation="TTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAE"
BASE COUNT           88 a          124 c          100 g           90 t
ORIGIN      
        1 ctccgctttg taaagaccat tgatgctctc cgccatcgcg ttgtcatacg agtcgcctgt
       61 acttcctgtt gatgccagta atccggcttc cttaagccgc tgtgtgtagg ccagcgatac
      121 atactgagaa cctttatcac tgtgatggac cgtgccggac ggtcgacggg cccataacgc
      181 ctgctccagt gcatccagca cgaatgtcgt ctccatggac gatgagaccc gccaccccac
      241 aatgtatccg gcaaacacat caatgatgaa cgccacatag acgaagccct gccatgtgct
      301 gacgtaagta aaatcagcca cccacagctg gtcaggtcgt tctgccacga actgacggtt
      361 tacgcggtcg cctgcggcaa cggctttccg gctgatggtc gt
//
LOCUS       DAZGCS010000492          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGCS010000492 DAZGCS010000000
VERSION     DAZGCS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.500"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..302
                     /locus_tag="ACHK01_005615"
     CDS             <1..302
                     /locus_tag="ACHK01_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO8100310.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>397
                     /locus_tag="ACHK01_005616"
     CDS             299..>397
                     /locus_tag="ACHK01_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100311.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGT"
BASE COUNT           94 a           86 c          137 g           80 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtaca
//
LOCUS       DAZGCS010000493          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGCS010000493 DAZGCS010000000
VERSION     DAZGCS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.501"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..332)
                     /gene="ler"
                     /locus_tag="ACHK01_005617"
     CDS             complement(<1..332)
                     /gene="ler"
                     /locus_tag="ACHK01_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO8100312.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLV"
BASE COUNT           98 a           91 c           66 g          137 t
ORIGIN      
        1 ttcacaagaa aatcttcttt cttcattcca ttcaacagtg cttctttaag ccagcgtggc
       61 tgtcggccaa ctcccgacca ggtctgccct tcttcattgc gataataaac tcctttcgat
      121 gagttccggc gagcaaggcc atcatcaggc acattagtat atcccagctc ttgtagggtt
      181 atattgtaat aagtaatctg cttttgaatc gactcaatta cactttgaat ttcctgctct
      241 cgcagtcgct ttgcttcctg ttgtaacact tcaatttgct caataagctg aatgtatggg
      301 cttgttgtgt gcgaattagt ttccatattc ataataaata atctccgcat gctttaatat
      361 attaagctat tagcaatctt atccttatca gg
//
LOCUS       DAZGCS010000494          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGCS010000494 DAZGCS010000000
VERSION     DAZGCS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.502"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>391)
                     /locus_tag="ACHK01_005618"
     CDS             complement(<1..>391)
                     /locus_tag="ACHK01_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100313.1"
                     /translation="TQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVG
                     IVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFV
                     FTKEATSRIGVGNLYRLMRGYAEGGYVG"
BASE COUNT           87 a          141 c           90 g           73 t
ORIGIN      
        1 cccacataac caccttccgc atagccgcgc atcagacggt aaagattccc cacacctatc
       61 cggctggttg cctctttcgt gaaaacaaac tccccgcggt gaactatccc cgcaggctca
      121 tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat ggaagttcgc cgccgcagcc
      181 tcaatggccg tccccgagga agcagatgca ccaccaccga aagcaccgcc aatggcgctg
      241 ccgatacgcc cgacaatgcc caccatggcc tgtttaagca ggatttctgt catcatggac
      301 agcaccgaac gggtgaatcc ccgccagtct gcctctgcac cggtcagcat cgccgccata
      361 ttctgtgcaa taccgtcaaa ggtctgcgtg g
//
LOCUS       DAZGCS010000495          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGCS010000495 DAZGCS010000000
VERSION     DAZGCS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.503"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..226
                     /locus_tag="ACHK01_005619"
     tRNA            151..226
                     /locus_tag="ACHK01_005619"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Glu,seq:ttc)
BASE COUNT          112 a           87 c           90 g          100 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaagt aaatagcaag gcgtttacgc gttgggagtg
       61 aggctgaaga gaataaggcc gttcgctttc tattaatgaa agctcaccct acacgaaaat
      121 atcacgcaac gcgtgataag caattttcgt gtccccttcg tctagaggcc caggacaccg
      181 ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg ctggtttgtg
      241 agtgaaagtc gccgacccca atatctcaaa acagactgtt aagtcttgtt tgagatattt
      301 gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt
      361 gagtctctca aattttcgca actctgaag
//
LOCUS       DAZGCS010000496          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGCS010000496 DAZGCS010000000
VERSION     DAZGCS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.504"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>389
                     /locus_tag="ACHK01_005620"
     CDS             <1..>389
                     /locus_tag="ACHK01_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100314.1"
                     /translation="AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQN
                     QEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSL
                     QEKIAGYQYVLANPGWTTGNGFMINHL"
BASE COUNT           99 a           99 c          125 g           66 t
ORIGIN      
        1 cgccgcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgagcggtgc
       61 gctgggactg gttggaggcg tacccggact ggtgatgctg ggagctgccg catggtacac
      121 gctgtaccag aatcaggaac aggccaggga gtctgcgcgc cagtatgcac tgacgataga
      181 tgaaatcgcg cataaaacgc catcaatgtc tctgcctgaa gcctcagata acgaagggcg
      241 aacacgggag gcgctgacag agcaaaaccg gctgattgat gagcaggcca gccgggtgaa
      301 atccctgcag gaaaaaatcg ccgggtatca gtatgtgctg gctaatccgg gctggacgac
      361 cggtaacggc ttcatgataa accatctga
//
LOCUS       DAZGCS010000497          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGCS010000497 DAZGCS010000000
VERSION     DAZGCS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.505"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>389
                     /locus_tag="ACHK01_005621"
     CDS             <1..>389
                     /locus_tag="ACHK01_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100315.1"
                     /translation="AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQN
                     QEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSL
                     QEKIAGYQYVLANPGWTTGDGFMINHL"
BASE COUNT           97 a           96 c          128 g           68 t
ORIGIN      
        1 cgccgcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgagcggtgc
       61 gctggggctg gttggtggcg tacccggact ggtgatgctg ggggctgcag catggtacac
      121 gctgtaccag aatcaggagc aggccaggga gtctgcgcgc cagtatgcac tgacgataga
      181 tgaaatcgcg cataaaacgc cgtcaatgtc tctgcctgaa gcctcagata atgaaggacg
      241 aacacgggcg gcgctgacag agcagaaccg gctgattgat gaacaggcca gtcgggtgaa
      301 atccctgcag gaaaaaatcg caggatatca gtatgttctg gcgaacccgg gctggacgac
      361 cggtgacgga ttcatgataa accatctga
//
LOCUS       DAZGCS010000498          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGCS010000498 DAZGCS010000000
VERSION     DAZGCS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.506"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..217
                     /locus_tag="ACHK01_005622"
     CDS             <1..217
                     /locus_tag="ACHK01_005622"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8100316.1"
                     /translation="SYERQGIPCPWRYYNDRDVRTIVELGKAIDFDVRTAIPFEGERH
                     NALDDARYQAKYVSAIWKKLIPSQADF"
     gene            289..>387
                     /gene="xisR"
                     /locus_tag="ACHK01_005623"
     CDS             289..>387
                     /gene="xisR"
                     /locus_tag="ACHK01_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8100317.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKA"
BASE COUNT          115 a           86 c           94 g           92 t
ORIGIN      
        1 ttcatacgaa cggcagggga tcccctgccc gtggcgttac tacaacgatc gcgatgtacg
       61 cacaatcgtt gagctgggga aagccataga cttcgatgtc agaactgcta tcccattcga
      121 aggtgagcgc cataatgcac ttgatgacgc ccgttatcag gcaaaatacg tttcagctat
      181 ctggaaaaaa ctgatcccga gtcaggctga tttttaatgt tcaaccgtcg tcggttgcaa
      241 ttggtattct gcaaccggcg ttcatctgat gtaagagata aagaatcgat gagcgaagta
      301 atcatgatcg tctctcctgg caaatgggta tccgaagagc agttaattgc acttaaagga
      361 atcaaaaagg gaacgctaaa aaaagcc
//
LOCUS       DAZGCS010000499          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGCS010000499 DAZGCS010000000
VERSION     DAZGCS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.507"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>385
                     /locus_tag="ACHK01_005624"
     CDS             <1..>385
                     /locus_tag="ACHK01_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8100318.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVA"
BASE COUNT           73 a          117 c          119 g           76 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggca ttcagccttc tcccgcacag gcacagaaca
      121 ttcgggagaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgttgccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg acagcacttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccgcgctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tgttgcctct gttgc
//
LOCUS       DAZGCS010000500          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGCS010000500 DAZGCS010000000
VERSION     DAZGCS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.508"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..357
                     /gene="cesT"
                     /locus_tag="ACHK01_005625"
     CDS             <1..357
                     /gene="cesT"
                     /locus_tag="ACHK01_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO8100319.1"
                     /translation="ISLSDANDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGP
                     YLCYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMK
                     IEEISSSDNKHYYAGR"
BASE COUNT          133 a           57 c           77 g          118 t
ORIGIN      
        1 atttcgttat ctgatgctaa tgacgaatat atgatgatct atggcgtctg tgggaaattc
       61 ccgacagata atcctaactt cgctcttgag attttgaatg caaacttatg gtttgcagag
      121 aatggtggac catacctgtg ttatgaatca ggagcacaat cgctgttgtt agcgttacgt
      181 tttccgctcg atgatgctac acctgaaaaa cttgagaatg aaatagaagt cgtcgttaag
      241 tcaatggaaa acctgtattt ggtattacat aatcagggaa taacattaga aaacgaacat
      301 atgaaaatag aggaaattag ttcaagcgat aataaacatt attacgccgg aagataaaag
      361 attatttatt aatataattt actat
//
LOCUS       DAZGCS010000501          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGCS010000501 DAZGCS010000000
VERSION     DAZGCS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.509"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            171..>385
                     /locus_tag="ACHK01_005626"
     CDS             171..>385
                     /locus_tag="ACHK01_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100320.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIA"
BASE COUNT          109 a           70 c          107 g           99 t
ORIGIN      
        1 tatttatgaa aattttccgg gatccatgtc cggtttctct gcaagttaac tatatgaaaa
       61 atataaaaac aggctgtccg tgaaccggac atgtgcaaaa aacggacatg taaaccggac
      121 atgaccggtt ttgttgtgat tgtgaggtga gagtttttgc gaggtgagga gtggctacgc
      181 agactgaagt tgccaggcat ttaagtctga ccgatcgcca gcttcgcaga ttgcagaaat
      241 tgccgggtgc cccgatatcg aataagcgag ggcaactgga tctggatgcc tggcgcgatt
      301 tttacatatc gtatctgagg agaagtaaaa acgatgtgcc tgatggcgat agcgaagacg
      361 actatgagga gaaattgctt attgc
//
LOCUS       DAZGCS010000502          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGCS010000502 DAZGCS010000000
VERSION     DAZGCS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.510"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..333)
                     /locus_tag="ACHK01_005627"
     CDS             complement(<1..333)
                     /locus_tag="ACHK01_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8100321.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQV"
BASE COUNT           85 a          142 c           79 g           76 t
ORIGIN      
        1 cacctgctct atcgtgcaac tgaaatcact gtaccgggca ttatccgtga cactccactc
       61 acggcacaca accctcaccg tccggttatg tttcggcggt cgccacaaaa aggcacggta
      121 accaccatgc cacgataaaa actcttccag ccagcgccgg gttgactcat ccgtcacccg
      181 gaacaccgcc tgaaacgtct tcagttgagg attcagccct gtggggcggc gctgttcata
      241 accgtcacca aaccgcaccc tcaccaccga cggcttctca ctcacctgca tcccttcacg
      301 cgggaccaga tgcagcgttt ttatctcagc cactcagcat tcctccgtca cgtcgcatgg
      361 acagcatcac cgcctgcacc cg
//
LOCUS       DAZGCS010000503          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGCS010000503 DAZGCS010000000
VERSION     DAZGCS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.511"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..>381)
                     /locus_tag="ACHK01_005628"
     CDS             complement(310..>381)
                     /locus_tag="ACHK01_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100322.1"
                     /translation="LWDSRYACQQKMRIFNLLSPKLR"
BASE COUNT          120 a           62 c           97 g          102 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata a
//
LOCUS       DAZGCS010000504          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGCS010000504 DAZGCS010000000
VERSION     DAZGCS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.512"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>371)
                     /locus_tag="ACHK01_005629"
     CDS             complement(<1..>371)
                     /locus_tag="ACHK01_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100323.1"
                     /translation="WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASAST
                     GTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGY
                     AEGGYVGGAGSPAQMRRTEGI"
BASE COUNT           82 a          140 c           86 g           63 t
ORIGIN      
        1 taatgccttc cgtccgccgc atctgcgccg gacttccggc accgcccaca taaccacctt
       61 ccgcatagcc ccgcatcagg cggtacaggt tgccgacgcc aatccggctg gttgcttcct
      121 tcgtgaagac aaactccccg cgatgaacaa ttcccgcagg ttcatattta ccccccgtcc
      181 ccgtaaatcc cccggtcgcg aaatggaagt tcgccgccgc agcctgaatg gccgtccccg
      241 tggaggcaga cgcaccacca ccgaaagcac cgccaatggc gctgccgata ctcccgacta
      301 tccccaccat cgcctgcttc agaaaaatct ctgtcagcat ggagagcaca gaacgggtga
      361 aaccacgcca g
//
LOCUS       DAZGCS010000505          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGCS010000505 DAZGCS010000000
VERSION     DAZGCS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.513"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(189..>369)
                     /locus_tag="ACHK01_005630"
     CDS             complement(189..>369)
                     /locus_tag="ACHK01_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8100324.1"
                     /translation="GLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVPRQKVAIIYD
                     VGVSTLYKKFPVGDK"
BASE COUNT          106 a           93 c           73 g           97 t
ORIGIN      
        1 ataaaaaata ttgatgcaga gctaagagat gctgtcaata agggattcga cattatcgac
       61 caagaagtaa agtcactaca aatgaaatta aacaaattag cttcatagaa tgctccactc
      121 cctgttcaat atttttacaa gcttatcagc cagccgtggc acgttcttgc atacgacgtg
      181 ctacggtttc atttatctcc gaccggaaac ttcttataca aagtcgatac gccaacatca
      241 taaatgatcg ccaccttctg gcgaggaact cctgatgcaa ttagtcgccc ggcctgcgcc
      301 cattgttctg gtgtaagttt gggacgacgt ccaccaattc gtccctgtgc gcgagcagct
      361 tccagtcca
//
LOCUS       DAZGCS010000506          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGCS010000506 DAZGCS010000000
VERSION     DAZGCS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.514"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..>366)
                     /locus_tag="ACHK01_005631"
     CDS             complement(105..>366)
                     /locus_tag="ACHK01_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100325.1"
                     /translation="SIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNV
                     QNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          135 a           74 c           54 g          103 t
ORIGIN      
        1 tgcatgaagc ttagaagact taaacaaaaa taaacaacat cattgtgatg ataaatataa
       61 aataggcatg gcgaaaaaaa atcaccacgc ctaaaatata ataattatgg tagcatcatt
      121 gatacataat ccacaccaat ccttgagcta tactgagacg ctatagcctg atatctttct
      181 gcataaccag ttctcagttg agatttaagt ttgagtcgga caggaacatt ttgcacgttg
      241 ccatccatat tacttaaaaa cacggcagaa ataatatttt tttcttcgcc atcaactgtt
      301 gttccatgat tcaacaccac catataatca acaacaggaa gcgttttatc cccttcgaaa
      361 atagag
//
LOCUS       DAZGCS010000507          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGCS010000507 DAZGCS010000000
VERSION     DAZGCS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.515"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>364
                     /locus_tag="ACHK01_005632"
     CDS             <1..>364
                     /locus_tag="ACHK01_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8100326.1"
                     /translation="ASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAE
                     MKTSITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPY
                     VAGIGAGIEDTDGQPLSNI"
BASE COUNT          113 a           83 c          107 g           61 t
ORIGIN      
        1 agccagtggc cgggccagca atgatgcaga agggtatctg gggctgtttc gggaaaaaat
       61 aggaaaactg catctggctc aggggctgtg ggagctgata gacaacagcc agcttgcgga
      121 tgagatggcg gagatgaaga cctccatcac cgaaacccgc aatgaaatca cacagacggt
      181 cagtaaaacg ctggaggacc agagcgccac catacagcag atacagcgcg tgcagaagga
      241 cacaaatgat gaccttgctg cactttacat gctgaaggta cagaaaacaa aaaatggcat
      301 accctatgtt gccggtattg gagcggggat tgaggatact gatggccagc ccctgagcaa
      361 cata
//
LOCUS       DAZGCS010000508          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGCS010000508 DAZGCS010000000
VERSION     DAZGCS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.516"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            139..>361
                     /gene="grlR"
                     /locus_tag="ACHK01_005633"
     CDS             139..>361
                     /gene="grlR"
                     /locus_tag="ACHK01_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO8100327.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLV"
BASE COUNT          119 a           46 c           83 g          113 t
ORIGIN      
        1 aacaggttgg aatatgagta tatttaatga tatattacat tgcaatctgg agaaaaagaa
       61 aggtctccgt tattcttgat atcgcttatg gatagaacaa attgaaagga gtgaggttag
      121 tatgaaactg agtgagttat gattatgaag gatggcatct atagcattat ctttattagc
      181 aatgaagatt cctgtgggga aggtatactg attaaaaatg gaaatttgat cactggcgga
      241 gatattgctt ctgtgtatca gggggtcctg tctgaagagg aggacatcat acttcatgtc
      301 catcgctata atcatgaaat tccctcggtg ctaaacattg aacaagatta tcaattagtt
      361 a
//
LOCUS       DAZGCS010000509          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGCS010000509 DAZGCS010000000
VERSION     DAZGCS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.517"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..>359)
                     /locus_tag="ACHK01_005634"
     CDS             complement(130..>359)
                     /locus_tag="ACHK01_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO8100328.1"
                     /translation="GQKSSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHL
                     RSTTVPATPEHTVRRSDYRATLGRTQPIDIE"
BASE COUNT           92 a           79 c           81 g          107 t
ORIGIN      
        1 atggcaacag atcgcgttcc cggtttgccg gccagtcctg gatatgctcc cgccgacaat
       61 attaatggcc aggaaatgtt taaaacaatg attctgtttc ctaaccatca agtgtatatg
      121 ctggctcaat tactcaatat ctatgggttg agttctaccc aaagtggctc tgtagtcgct
      181 tcttcttaca gtatgttcag gtgttgcagg cacagttgtg gagcgtagat gtgttgttgg
      241 tttaccatgc tctggtacat aaaaaacgcc agtgccatgg taacctgata tagaaacgtt
      301 aattccgcga cgttcaaact cggcatagac gtgttctgct aaagaactct tttgaccaa
//
LOCUS       DAZGCS010000510          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGCS010000510 DAZGCS010000000
VERSION     DAZGCS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.518"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHK01_005635"
     CDS             <1..>356
                     /locus_tag="ACHK01_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8100329.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZGCS010000511          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGCS010000511 DAZGCS010000000
VERSION     DAZGCS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.519"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>352
                     /locus_tag="ACHK01_005636"
     CDS             <1..>352
                     /locus_tag="ACHK01_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO8100330.1"
                     /translation="LLPDKNSRNGLKLLQKQADLLKIIMEDTSVTENTFKNIEMAITD
                     IKREYYSHTVDIEKNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYLWVDEKAFGAAK
                     FTSVLKQIAFDLAC"
BASE COUNT          127 a           60 c           63 g          102 t
ORIGIN      
        1 atcttttacc ggataaaaac agtcgtaatg gtctaaaact tctacagaag caagctgatt
       61 tactaaaaat aatcatggag gatacctctg ttacagaaaa tacctttaaa aacatagaga
      121 tggctataac agatataaaa agggagtact attcgcatac agttgatatt gagaaaaata
      181 ttcatgccat atgggttgcg ggttccccac ctgaaagcat ctcggactat attaagactt
      241 ttctcaaaac ctacaaagag tttacttact atctttgggt tgatgaaaaa gcatttggag
      301 ccgcgaaatt taccagtgtc ttaaaacaaa ttgcatttga tttagcatgc ag
//
LOCUS       DAZGCS010000512          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGCS010000512 DAZGCS010000000
VERSION     DAZGCS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.520"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>347
                     /locus_tag="ACHK01_005637"
     CDS             <1..>347
                     /locus_tag="ACHK01_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO8100331.1"
                     /translation="TPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGGIQLSDGVFKI
                     TKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGV
                     AMTGTGNPFLMSY"
BASE COUNT           73 a           92 c          102 g           80 t
ORIGIN      
        1 acgccatcgg gtccgtctgc agatacgtcc gttcgcacaa tctccctgct gccggcagcc
       61 ggagaggctg ctgcgcaggg ctggagcatt aaggatggcg gaattcagtt gtcagatggt
      121 gtatttaaga tcaccaagca gagcaataaa acctggtccc tgacgcatcc ggtggatgac
      181 gcaattaccc tgctgacaca gggcggcaga ctgacctgta agttccgcct gtcaggcgca
      241 ctgaccaaca atcagttcgg gctggggatt tatctgtata cggatgctcc cgttcctgat
      301 ggtgtggcga tgacgggtac cggtaatccg ttcctgatgt cgtactt
//
LOCUS       DAZGCS010000513          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGCS010000513 DAZGCS010000000
VERSION     DAZGCS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.521"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..>346
                     /locus_tag="ACHK01_005638"
     CDS             60..>346
                     /locus_tag="ACHK01_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO8100332.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLT"
BASE COUNT          125 a           61 c           65 g           95 t
ORIGIN      
        1 actctgtgaa tgggataaac attattttaa actccaatct tgcgtaagtg aaagttgata
       61 tgtcattagt aggatataat agtaacatca taataaccat cgcgctgata tcgttgctct
      121 ctgcatgcac cagagaaaat tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc
      181 aagcgatagt agatgcaaga cagttcttta gcgaacaccc ggaatatata tcatcagcag
      241 aactggagca aaagctatat cgcgaatttg cacaggttac tacagttcca gcttataaag
      301 agatgagtat gtatcaattg cttatcattt cacaagatcg tttaac
//
LOCUS       DAZGCS010000514          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGCS010000514 DAZGCS010000000
VERSION     DAZGCS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.522"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>344
                     /locus_tag="ACHK01_005639"
     CDS             <1..>344
                     /locus_tag="ACHK01_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100333.1"
                     /translation="GLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQ
                     AAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSAL
                     TKTERELALSRL"
BASE COUNT           83 a           84 c          110 g           67 t
ORIGIN      
        1 agggcttgct caggcaacag agcagcttgc cgttgagcag tcccgtctgg cacagatgca
       61 ggaaaaagcg cagtccattc aggatgtgct tgccgggctg gaagaccgtc gtgtggcgtt
      121 aattcgtcag caggcggcag agcagaataa ggtgtaccag tccatgctgg ttatgaacgg
      181 tcagcatacg gaattcaacc gtctgctggg gctgggtaat gaactgcttc agcagcggca
      241 gggactggtg aatgtgccgt tacggctgcc acaggccact ctggatgata aacagcagag
      301 tgccctgaca aaaacagagc gtgagctggc cctgtccaga ctga
//
LOCUS       DAZGCS010000515          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGCS010000515 DAZGCS010000000
VERSION     DAZGCS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.523"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>344
                     /locus_tag="ACHK01_005640"
     CDS             <1..>344
                     /locus_tag="ACHK01_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8100334.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZGCS010000516          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGCS010000516 DAZGCS010000000
VERSION     DAZGCS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.524"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..306
                     /locus_tag="ACHK01_005641"
     CDS             4..306
                     /locus_tag="ACHK01_005641"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8100335.1"
                     /translation="MLLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKGKIK
                     NNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQE"
BASE COUNT          108 a           60 c           77 g           94 t
ORIGIN      
        1 aaaatattgt tagtttgcgc tgcgggcatg tcaaccagta tgctggttaa acgtatgatt
       61 gatcatgcta acgctatttc acttgaagtt aatatttccg cattggcgat tgcagaggct
      121 aaagggaaaa ttaaaaataa cgaagttgac gttgttttac ttggcccgca agttcgcttt
      181 cagaaaccag agattgaagc cgttgcacag gggaaaatgc ctgtagccgt tatcgagatg
      241 aaagactacg gcaccatgaa cggacaggca gttcttgaat ttgcgatgaa actactgcaa
      301 gagtaagaat ctaatattat taaactatta cctgaacta
//
LOCUS       DAZGCS010000517          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGCS010000517 DAZGCS010000000
VERSION     DAZGCS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.525"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            229..336
                     /locus_tag="ACHK01_005642"
                     /pseudo
     CDS             229..336
                     /locus_tag="ACHK01_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           74 a          103 c           64 g           95 t
ORIGIN      
        1 tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct cactaccatg
       61 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
      121 gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat
      181 ctttgtgcct acggcacacc gatgatcttt ccctgtgatc tttccctgaa tctgcccctg
      241 agaacctgaa tcaaacgtct gactcgcaga accatctgtt tctcgcgctc tgtagaactt
      301 catgaaaaag tcatcggtgc ttttatcgaa aaatac
//
LOCUS       DAZGCS010000518          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGCS010000518 DAZGCS010000000
VERSION     DAZGCS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.526"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>335
                     /locus_tag="ACHK01_005643"
     CDS             <1..>335
                     /locus_tag="ACHK01_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100336.1"
                     /translation="SRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETA
                     TGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLA
                     SCGLTVEQM"
BASE COUNT           71 a           96 c          118 g           50 t
ORIGIN      
        1 tgtcacggaa accgcaagtg acctggtacg gctgggacgg cgaccggctg accacgatac
       61 agaacgacag gagccgcatc cagacgattt atcagccggg gagcttcacg ccactcatca
      121 gggttgaaac cgccaccggt gagcaggcga aaacgcagcg ccgcagcctg gcggataccc
      181 ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg ctggtgcaga
      241 tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct
      301 ggctggcatc gtgcggcctg accgtggagc agatg
//
LOCUS       DAZGCS010000519          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGCS010000519 DAZGCS010000000
VERSION     DAZGCS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.527"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..>333
                     /locus_tag="ACHK01_005644"
     rRNA            1..>333
                     /locus_tag="ACHK01_005644"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           96 a           72 c          103 g           62 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agc
//
LOCUS       DAZGCS010000520          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGCS010000520 DAZGCS010000000
VERSION     DAZGCS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.528"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..328
                     /locus_tag="ACHK01_005645"
                     /pseudo
     CDS             1..328
                     /locus_tag="ACHK01_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          110 a           60 c           54 g          104 t
ORIGIN      
        1 atcccacagg cagaaaatgg tggttacgat ttttcacacc ttagcctgaa aggtatcgtg
       61 attaaagatg aagatttatc caattcgaat tttgcaggtt gcagactaca aaactctatt
      121 ttccaggact gtaatatgta tagaacgaat tttattgcgc cataatggaa aaaatacttt
      181 ttgataattg tattctcgat gactcatatt tcgctcatgt aaaaatgact gacgggactc
      241 taaatgcatg ctccgctatg cattttcaat tctacaatgc agcaatgaat agagccaata
      301 ttaaaaatac cttccttgac tattcaaa
//
LOCUS       DAZGCS010000521          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGCS010000521 DAZGCS010000000
VERSION     DAZGCS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.529"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..205
                     /locus_tag="ACHK01_005646"
     CDS             <1..205
                     /locus_tag="ACHK01_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8100337.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     RNATYPVLIDSIVVEVNSTDTAA"
BASE COUNT           92 a           70 c           86 g           75 t
ORIGIN      
        1 tttccgcttt gccgggaata acagccttca ggtgacgccg gttattgatg gtcaggatgg
       61 cacaccgttc acgctgacgc agtcaccggt cagtgcattt gcggcggata aactgcatgt
      121 gacagacatt accaggaatg cgacttaccc ggtactgata gacagcattg tggtggaagt
      181 gaacagcaca gacactgcgg catgataaaa aaaccgccag cgacaggaat ggacgctggc
      241 ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct ggttttttaa
      301 tgaaaacagt tcttattgtc aac
//
LOCUS       DAZGCS010000522          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGCS010000522 DAZGCS010000000
VERSION     DAZGCS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.530"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>319)
                     /locus_tag="ACHK01_005647"
     CDS             complement(<1..>319)
                     /locus_tag="ACHK01_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100338.1"
                     /translation="NFIRGVFLFPVSAFDKAEHHKTTLHSILIKVFWPQIRDGYINVF
                     SVSTLYRRKGLRGDLFRLAYHYVYEHSKNHQVKMQNYVALYGFCRNITFVFLASVWLL
                     MFLL"
BASE COUNT          128 a           56 c           53 g           82 t
ORIGIN      
        1 gagcaggaac ataagcaacc agactgaagc gagaaaaaca aatgtaatgt ttctacaaaa
       61 accatacaaa gcaacatagt tttgcatttt cacctgatga tttttagaat gttcatacac
      121 ataatgataa gcaaggcgaa aaagatcgcc cctcaagccc ttacgtcgat atagcgtaga
      181 tacagaaaaa acatttatat aaccatctcg tatttgcggc caaaagactt taataagtat
      241 agaatgcagt gtggttttat gatgttctgc tttatcaaaa gctgatacag gaaataaaaa
      301 aactccacga ataaaatta
//
LOCUS       DAZGCS010000523          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGCS010000523 DAZGCS010000000
VERSION     DAZGCS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.531"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>319)
                     /locus_tag="ACHK01_005648"
     CDS             complement(<1..>319)
                     /locus_tag="ACHK01_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO8100339.1"
                     /translation="KLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLD
                     ADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINL
                     REWL"
BASE COUNT          121 a           69 c           39 g           90 t
ORIGIN      
        1 taaccattcc cgtaaattaa tatacattac accagagtta aaatagctac cgttaaaatc
       61 ctcattacac aaacgctcag cgcttttact ttgcatagca tctacatcaa ttaccactgc
      121 cccatattca tctttaaact ctaatgctat taactcgttc aatgaaccct tacagacgat
      181 atcagcatca agatataata atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa
      241 acgataataa atagatactg gccagatatt tgattgaggt agcgccttca atggctcaga
      301 atcaatatga tataattta
//
LOCUS       DAZGCS010000524          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGCS010000524 DAZGCS010000000
VERSION     DAZGCS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.532"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>314)
                     /locus_tag="ACHK01_005649"
     CDS             complement(<1..>314)
                     /locus_tag="ACHK01_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO8100340.1"
                     /translation="LTTYLFMSSGAVTARNVQTFSPTINNESRIRLPRALPVGHPYPE
                     EHKASGFSPFMMGGLSGDILPEILTGNGPSIFFNGKHNNQHDGAFGKIIDFTQNGNKI
                     SA"
BASE COUNT           91 a           67 c           54 g          102 t
ORIGIN      
        1 tgcacttatt ttatttccat tttgggtaaa atctattatt tttccaaaag ctccatcatg
       61 ttggttatta tgttttccgt taaaaaatat agatggtcca ttccccgtta aaatttccgg
      121 aagaatatca ccactcagcc cccccatcat aaaaggggag aaaccagaag ccttatgttc
      181 ctcaggatat ggatggccta ctggcaatgc tctcggcaat cgaatccttg actcattatt
      241 tattgttgga gaaaaggttt gaacatttct ggctgtcacc gctcctgatg acataaacaa
      301 atatgtcgtc aata
//
LOCUS       DAZGCS010000525          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGCS010000525 DAZGCS010000000
VERSION     DAZGCS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.533"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..169)
                     /locus_tag="ACHK01_005650"
     CDS             complement(89..169)
                     /locus_tag="ACHK01_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100341.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(205..>303)
                     /locus_tag="ACHK01_005651"
     CDS             complement(205..>303)
                     /locus_tag="ACHK01_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100342.1"
                     /translation="PLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          113 a           53 c           47 g           90 t
ORIGIN      
        1 agcatgcttt tattttgagc attggtaaca gagcaataat gcattgtgca aggtaaacta
       61 aaatagataa caaaaagatg gaacctcgtt aatcataaaa caataaaatt aaatattctc
      121 gcagtatatg gcagtctaaa gcatcaaagt tttgatcaac atctttcatt ttagacatct
      181 ccttgcaatg taaaatatca tgtatcaaca acatctggtg caaatccatg agtcgaactc
      241 gacattcatc ttagctcgat tattattcct tcgtacagtc gatgtaaaca atacagaaag
      301 agg
//
LOCUS       DAZGCS010000526          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGCS010000526 DAZGCS010000000
VERSION     DAZGCS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.534"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>303
                     /locus_tag="ACHK01_005652"
     CDS             <1..>303
                     /locus_tag="ACHK01_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO8100343.1"
                     /translation="RIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDR
                     HGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLG"
BASE COUNT           69 a          103 c           83 g           48 t
ORIGIN      
        1 ccgcatcccg tatgccacag acccggcagg taaccgcctg cccgacccgg agctgcaccc
       61 ggacagcacc ctcagcatgt ggccggataa ccgtatcgcc cgtgacgcgc actatcttta
      121 ccggtatgac cgtcacggca ggctgacaga gaaaaccgac ctcatcccgg aaggggttat
      181 ccgcacggat gatgagcgga ctcaccggta ccattacgac agtcagcacc ggctggtgca
      241 ctacacgcgg acacaatatg cagagccgct ggtcgaaagt cgctatcttt acgacccgct
      301 ggg
//
LOCUS       DAZGCS010000527          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGCS010000527 DAZGCS010000000
VERSION     DAZGCS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.535"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>298)
                     /locus_tag="ACHK01_005653"
     CDS             complement(<1..>298)
                     /locus_tag="ACHK01_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100344.1"
                     /translation="NGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAA
                     RNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERL"
BASE COUNT           49 a          110 c           79 g           60 t
ORIGIN      
        1 tcaggcgttc ctgtgtggcc gccagacggg cctctgctgc aatctgcatc tccgtcccgc
       61 gggctgccgc cacggcctgc tgagcacggt acacggctgc tcttgcccgc gccgtggcaa
      121 tctgcgttcc cctgaactgt gcttccgcca gtgcaacttc attacgtgca gccgtcacaa
      181 gtcctgccgt ggcagacatc gctccggagg ccatattgcc aaagtaccgg gcaaccccga
      241 cggcaaccag tgcccccacg gctgttgcca cattatcaat ctgtccggca acaccgtt
//
LOCUS       DAZGCS010000528          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGCS010000528 DAZGCS010000000
VERSION     DAZGCS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.536"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>298
                     /locus_tag="ACHK01_005654"
     CDS             <1..>298
                     /locus_tag="ACHK01_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8100345.1"
                     /translation="MACEEANGREQLAATLAAMPEMTVEKARPILAASPQADAGPSLR
                     DQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGSGKAEPVSASTTALFER"
BASE COUNT           63 a           82 c          104 g           49 t
ORIGIN      
        1 ttatggcgtg tgaagaagcg aacggtcgtg aacaactggc ggcaacgctg gcggcgatgc
       61 cggagatgac ggtggaaaaa gcccgcccga tcctggctgc ttcaccgcag gcggatgccg
      121 gaccctcact ccgtgatcag atcatggcac tggatgaggc aaaaggggct gaggcgcagg
      181 ctgaacagct ggctgcctgc ccgggaatga ctgtggagag cgcccgggct gtgctggctg
      241 cgggatcagg taaggcagaa ccggtctctg catccacaac cgccctgttt gaacgcat
//
LOCUS       DAZGCS010000529          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGCS010000529 DAZGCS010000000
VERSION     DAZGCS010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.537"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHK01_005655"
     CDS             complement(<1..242)
                     /locus_tag="ACHK01_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8100346.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           83 a           62 c           66 g           84 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgca
//
LOCUS       DAZGCS010000530          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGCS010000530 DAZGCS010000000
VERSION     DAZGCS010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.538"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>290)
                     /locus_tag="ACHK01_005656"
     CDS             complement(<1..>290)
                     /locus_tag="ACHK01_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8100347.1"
                     /translation="RYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFI
                     AARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDG"
BASE COUNT           76 a           93 c           71 g           50 t
ORIGIN      
        1 ccgtcaatgg ccattcttcc ggcaccaatc cactctgccc gtgaccaggc tgatcgcgcc
       61 tgataaaaat caaaacgcgc ccgtggcgga cggataatcc cccgaagaag tgcctcttcc
      121 agccagcagg aaaacatctg cgtggccagc cgggccgcaa taaattttcg ccgccccata
      181 aaatagcgcc acgactcatt ggcagaggcc ctggcacttg aataactgac cttcgagtaa
      241 tcacgggaca actgttcgta ggaaacgcca agaccggcgg cgatataccg
//
LOCUS       DAZGCS010000531          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGCS010000531 DAZGCS010000000
VERSION     DAZGCS010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.539"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           76 a           59 c           79 g           74 t
ORIGIN      
        1 tgcgtcagtc agtgccatag ttcagccttc tgaatgcgtt aaagagggta caaattctgt
       61 cgaactgggg tatgcccgca attataccca cacaatgagg ttgatgtggg gagaatttgg
      121 ttgagttcgg tagaattgat ttggaggaag aacacttaaa tcatggtgtt ctgaaacgaa
      181 aaacggacct ccgtggaggt ccgttgatat gaatttggtg cccggactcg gaatcgaacc
      241 aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccg
//
LOCUS       DAZGCS010000532          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGCS010000532 DAZGCS010000000
VERSION     DAZGCS010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.540"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..287
                     /locus_tag="ACHK01_005657"
                     /pseudo
     CDS             1..287
                     /locus_tag="ACHK01_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          109 a           48 c           47 g           83 t
ORIGIN      
        1 caaaccatca agataagcat tcagataaag ttaacagtca tcaacaccat ggcaaggttg
       61 ataaaaaata tcgatcaaac acagtttaag ttaatatgct gaatgacttt tcgcaaatag
      121 ataacgattt aggcctgaat atcatctatt tcctgcaaca tggtcactgg agggtaaatg
      181 aacatagtct tcagatggag aaaatatggt tttacaatag tgagccatct atagatattc
      241 aagaatataa tagtttcgcc aagaatacta ctaacacgtt tatcttt
//
LOCUS       DAZGCS010000533          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGCS010000533 DAZGCS010000000
VERSION     DAZGCS010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.denovo.541"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>287
                     /locus_tag="ACHK01_005658"
     CDS             <1..>287
                     /locus_tag="ACHK01_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8100348.1"
                     /translation="ANEKISQQRRDLWKAENQYAVLKEAATKRQLSEQEKSLLTHEKE
                     TLEYKRQLAELGDKVEHQKRLNELAQQAARFEQQQGAKQAAISAQARGLTD"
BASE COUNT           85 a           70 c           94 g           38 t
ORIGIN      
        1 gtgcgaatga gaaaatcagc cagcagcgtc gcgatttatg gaaagcggaa aatcagtatg
       61 cggtcctgaa agaggcagcc acgaaacggc agttatctga gcaggaaaaa tccctgctga
      121 cccatgagaa agagacgctg gagtacaaac gccagctggc tgagctggga gacaaagttg
      181 aacaccagaa acggctgaat gagctggcac agcaggctgc gcggtttgag cagcagcagg
      241 gcgcgaagca ggcggcaatc agtgcccagg cgcggggcct caccgac
//
LOCUS       DAZGCS010000534        15986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.109, whole genome shotgun sequence.
ACCESSION   DAZGCS010000534 DAZGCS010000000
VERSION     DAZGCS010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.109"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..408
                     /locus_tag="ACHK01_005659"
     CDS             <1..408
                     /locus_tag="ACHK01_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFO8100349.1"
                     /translation="LFDTEFGPMAQILVGATIVGSIETVWAGTITPPREGIIKRWTWP
                     AGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSVTKIGQPLAVSTET
                     FVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            430..3753
                     /gene="mscM"
                     /locus_tag="ACHK01_005660"
     CDS             430..3753
                     /gene="mscM"
                     /locus_tag="ACHK01_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8100350.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3904..5406)
                     /gene="yjeM"
                     /locus_tag="ACHK01_005661"
     CDS             complement(3904..5406)
                     /gene="yjeM"
                     /locus_tag="ACHK01_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8100351.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(5625..6602)
                     /gene="epmA"
                     /locus_tag="ACHK01_005662"
     CDS             complement(5625..6602)
                     /gene="epmA"
                     /locus_tag="ACHK01_005662"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8100352.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            6927..8735
                     /gene="frdA"
                     /locus_tag="ACHK01_005663"
     CDS             6927..8735
                     /gene="frdA"
                     /locus_tag="ACHK01_005663"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8100353.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8728..9462
                     /gene="frdB"
                     /locus_tag="ACHK01_005664"
     CDS             8728..9462
                     /gene="frdB"
                     /locus_tag="ACHK01_005664"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8100354.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            9473..9868
                     /gene="frdC"
                     /locus_tag="ACHK01_005665"
     CDS             9473..9868
                     /gene="frdC"
                     /locus_tag="ACHK01_005665"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8100355.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            9879..10238
                     /gene="frdD"
                     /locus_tag="ACHK01_005666"
     CDS             9879..10238
                     /gene="frdD"
                     /locus_tag="ACHK01_005666"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8100356.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            10301..11434
                     /locus_tag="ACHK01_005667"
     CDS             10301..11434
                     /locus_tag="ACHK01_005667"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8100357.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            11523..12056
                     /gene="blc"
                     /locus_tag="ACHK01_005668"
     CDS             11523..12056
                     /gene="blc"
                     /locus_tag="ACHK01_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8100358.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(12053..12370)
                     /gene="sugE"
                     /locus_tag="ACHK01_005669"
     CDS             complement(12053..12370)
                     /gene="sugE"
                     /locus_tag="ACHK01_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8100359.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(12552..12698)
                     /gene="ecnB"
                     /locus_tag="ACHK01_005670"
     CDS             complement(12552..12698)
                     /gene="ecnB"
                     /locus_tag="ACHK01_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8100360.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(12809..12934)
                     /gene="ecnA"
                     /locus_tag="ACHK01_005671"
     CDS             complement(12809..12934)
                     /gene="ecnA"
                     /locus_tag="ACHK01_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8100361.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(12986..13552)
                     /gene="efp"
                     /locus_tag="ACHK01_005672"
     CDS             complement(12986..13552)
                     /gene="efp"
                     /locus_tag="ACHK01_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8100362.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            13594..14622
                     /gene="epmB"
                     /locus_tag="ACHK01_005673"
     CDS             13594..14622
                     /gene="epmB"
                     /locus_tag="ACHK01_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8100363.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            15012..15881
                     /locus_tag="ACHK01_005674"
     CDS             15012..15881
                     /locus_tag="ACHK01_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8100364.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4060 a         4140 c         4112 g         3674 t
ORIGIN      
        1 ctgttcgata ccgaatttgg cccgatggcg cagattctgg tcggggcgac gattgtaggc
       61 agcattgaga cggtctgggc gggcaccatt acgccgccgc gcgaaggtat catcaagcgc
      121 tggacctggc ctgccgggga aaacgacggt tctgtggcac tgctgaaagg ccaggaaatg
      181 ggccgcttta aactcggctc taccgttatc aacctgtttg caccgggtaa agtgaatctg
      241 gttgagcaac tggaaagcct gtctgtcacg aaaattggcc agccgctggc agtatctacc
      301 gaaacctttg ttacgccaga cgctgaaccc gccccacttc ctgctgaaga gatcgaagca
      361 gaacacgacg ccagcccatt ggttgacgat aaaaaagacc aggtctaatc catcaaagga
      421 aacgctgacg tgcgcctgat tatcactttt ctgatggcct ggtgcctcag ttggggggcg
      481 tacgccgcga cggcccccga tagcaaacaa atcactcagg aactggagca ggcaaaagcg
      541 gcgaaacccg cacagccgga agtcgtagag gcgctccagt ctgccttaaa tgcgcttgag
      601 gaacgaaaag gttcccttga gcgcatcaaa caatatcagc aagttatcga taattatccg
      661 aaactctccg ctactctgcg cgcacaatta aacaacatgc gtgacgagcc gcgcagcgtg
      721 tcgccgggaa tgtctaccga cgcgctgaat caggaaattc tccaggtcag cagccagttg
      781 ctggataaaa gccgtcaggc ccagcaagag caggagcgcg cccgcgagat tgccgattcg
      841 ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc agttaaatga gatcgagcgc
      901 cgcctgggaa cgcttactgg caataccccg ctcaatcagg cacaaaattt cgcgttgcag
      961 tctgactccg cacgcctgaa agcgctcgtt gatgaactgg aactggcgca gctgtctgcc
     1021 aataaccgtc aggaattagc gcgcttacgc tcagagctgg cggaaaaaga gagccagcaa
     1081 ctggatgcgt atttgcaggc cttacgtaat cagttgaaca gccaacgtca gcttgaggcg
     1141 gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca gcgccgattt gccgaaagac
     1201 atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg cactgaatca acaggcgcag
     1261 cggatggatc tcgttgcctc gcaacagcgc caggccacca gccagacgtt acaggttcgg
     1321 caggcgctga atacgctgcg tgaacagtcg caatggctgg gatcgtccaa cctgctcggc
     1381 gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc cgaaaccaca acagcttgat
     1441 accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg aggatctgct taataaacag
     1501 ccgctgctac ggcaaattca tcaggccgac ggtcagccgc tgaccgccga gcaaaaccgt
     1561 attctggaag cacaactgcg cactcagcgt gagttgctga actcattgtt gcagggtggc
     1621 gacacgctac tactggaact gaccaagctg aaagtctcca acgggcaact ggaggatgcg
     1681 ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct ggacctctga cgtgcgcccg
     1741 atgaccatcg cctggccgct ggaaatcgcc caggatctgc gtcgtctcat ttcgctggac
     1801 accttcagtc agttgggcaa agccagtgtg atgatgctga ccagcaaaga gacgattttg
     1861 ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt actcgcgccg ctatttcacc
     1921 cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga ctcaggatca cttctggctg
     1981 acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac cgctgccggt gctgtggatg
     2041 acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc tggcggtcgc gattggcgat
     2101 ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga tttgcgccac ctttgcccgc
     2161 ccgaacggct tgtttatcgc tcattttggc tggccgcgcg aacgtgtttc ccgtgggatg
     2221 cgctactacc tgatgagcat cgggcttatt gtgccgctga ttatggcgct gatgatgttc
     2281 gataacctcg acgaccgtga attctccggt tcgctgggac ggctttgctt tatcctcatt
     2341 tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg ccgggatccc gctgtacctc
     2401 aacaaagagg gcagcggcga caacattacc aaccatatgc tgtggaacat gatgattggc
     2461 gcaccactgg ttgccattct ggcgtcagcg gtgggttatc tggcaacggc gcaggcactg
     2521 ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc tactggtggt ttatcacgtt
     2581 atccgccgct ggatgctgat ccagcgtcgc aggctggcgt ttgaccgggc gaagcatcgc
     2641 cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg aagcgcatca tcacagtagc
     2701 ccggaagggg caattgaagt cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcaa
     2761 tccttgcggc tggtgcgctc aattttgatg ttgatcgccc tgctttctgt cattgtgctg
     2821 tggtcagaaa tccattccgc tttcggcttc ctcgaaaata tttcgctgtg ggatgtcacc
     2881 tccacggtac agggcgtaga aagtctggag ccaattaccc tcggtgcggt gctgattgcc
     2941 attctggtgt ttatcatcac cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg
     3001 gcgattttgc agcacctgga tttaacgccg ggtacgggct acgccatcac caccatcacc
     3061 aaatatctgc tgatgctgat tggcgggctg gtcggcttct cgatgattgg tattgagtgg
     3121 tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc tcggttttgg tttgcaggag
     3181 attttcgcca actttatctc tggcctgatt atcctgttcg aaaaaccgat tcgcattggc
     3241 gatacggtaa cgatccgcga tcttaccggt agcgtgacga aaattaacac ccgcgccacc
     3301 accatcagcg actgggaccg taaagagata atcgtgccga acaaggcgtt tattaccgag
     3361 cagtttatca actggtcgct ctctgactcg gtcacgcgcg tggtgttgac gataccggcc
     3421 cctgccgatg ccaatagcga agaagtgacg gaaatcctgc tcaccgcagc gcgtcgctgc
     3481 tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg tggatctgca acaggggatt
     3541 cagattttcg agctgcgtat ttacgccgct gagatgggtc accgtatgcc gatacgccat
     3601 gagatccacc agctgattct ggcaggcttc cacgcccacg gtatcgatat gccattcccg
     3661 cccttccaga tgcgtctgga aagcctcaac ggtaagcaaa cggggagaac attaacttct
     3721 gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga agctgccaaa tgatgtcaac
     3781 gtggctcatt tagcagctta acatcatctt cagttatcac cggccctgga gagtatcatc
     3841 catacgcatt acttcctgac aactcatcgc cggagagacg atgagttgca ggatttcata
     3901 tgatcagtcg agagctaact caggtttatt cgccatgcga ctgcaatagt tctggtaaat
     3961 cgccatcgct aacagcgaga agaagacagg gccgccaatc atccacaatg tgctgtccca
     4021 gtctccggct tcaaccacag gttgaatgat ggtgaagacg ttcgcaaatg tcaccaccag
     4081 tacgacaacc accgtcgcaa tcattgccga catacgcgtt ttgaaaatca caaatggtct
     4141 gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc gcgaggaaca ggtaaggaag
     4201 cgtcatagac acgttcgcca tcagcgtcag cttgttaaag aacgccgatg cggtaccgcc
     4261 accaaacgaa accagcagga tgaagacagt aaccaacccg cactgcatcc acatggcgat
     4321 agaaggcatc cccatcgcat tcaggcgcgt catcggttcc ggccacaacg ctttcggcgt
     4381 cccctggatg atggctttca gcggtgaata gcaaagcgta aagaacgccc cggtataagc
     4441 gaggaacatc gaaagtccgg taatacgcgc aaaccataca cccagcgaca atgacgcttc
     4501 aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg ctcttcatca gcacataggt
     4561 aatattgccg aggttaacgg aaccattact taatacctgc tgccagtttg tgctgacacc
     4621 ccataaaaat attgccagcg aataaccgat tgaaataaca atagcggcaa aaacaatacc
     4681 tttggcaaag ttcttttctg gattttccgt tttatcgacc agaccaccaa ccgcttcaat
     4741 tccgccatag gcaaaaatag caaataccac aaacgatagc attgccagac cggactgata
     4801 acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg aaatgcccac catttaataa
     4861 caaaatagta atgcttacta acagcaatac taaattcaga cacattactg caataccgcc
     4921 caccgcagta atgcgggcaa ttttattaat cccctttgaa gcaacgacgg tgaccagaat
     4981 catccatgct actgccagca gaccaaccac ctgcgtaggc tccagtccgg caatacgcca
     5041 gtgctgggtc atgtcgctac catagaggaa tgttgagaac ggtacccaaa ctttcgcgga
     5101 ggtactcacc atccagatga tataagagga aaaccacata aacgtaccaa tgaaggcaaa
     5161 acgtggtccg acactattat tcatccagga atagatacca ccttcttctt tgcgataagc
     5221 agctcccatt tcagccatca ttaaggcgaa tggaataaag aataacaatg cagaaaatat
     5281 ataaaaggga atcgcactat aacccattaa gtaataagcc gatgggctat tggcaaatcc
     5341 aaatacggaa gtaaagatca tcaatatgag tcctatcaga ctcatctttt ttatcgtgtg
     5401 aggcatgaaa ccatccttca tttcagcgcg tgtataacac gtgcccactg aactgcactg
     5461 cgcaacattg agcaggcagc gtaaaaacca atacagaatt gatacctgga tgatatcaaa
     5521 tatccagtct taaattgtgg cctttaacgg aaaaatgaaa acatatgcta cagaatggcg
     5581 cttatcacgc cattcttcgc tgttaattca gtaatttttc agaattatgc ccggtcaacg
     5641 ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg ccaacatcac cagacgatca
     5701 acacctaatg ccacgccgga acagtcaggc ataccgactt tcaaggcttc aatcagattc
     5761 tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct tacggttatc ttgttcaaag
     5821 cgttgttgct gctcacgggc atccgtcaat tcatggaaac cattcgccag ctcaatacct
     5881 ttataataaa cctcaaagcg ttcagcgacc cgatgatctt cggtactgat ttgcgccagt
     5941 gatgcctggc tggctggaaa gtggtacaca aaggtcggtt tttctttgcc aatatttggc
     6001 tctacgccaa aggtaaacag caattgtagc agcgtgtcgc ggtcttcttc ggtatcagca
     6061 acattgctca aatccagttt cgctgcgact tcccgcagtt gcgttttgtc ggcagagagc
     6121 gggtcaattt ccagataacg caagaaagct tgttgataag aaaggctttc tgctgccggg
     6181 cagtccagca cctgttgtaa gagatcgtcc acctcgttca tcaaccggta catatcatag
     6241 tgcggtcgat accactccag catagtgaac tcagggttgt gataacgccc catctcttca
     6301 ttacggaagc tgcggcacag ctggaatacc ggcccacaac cggcaaccag caggcgtttc
     6361 atatggtatt ccgggctggt cattaaccag agattcatcc cctgcgaatg cccggggcca
     6421 acgaaacgtg tctcaaacgg gaccaaatga atatcggtta ccgtcgcctg gctcatacaa
     6481 ggcgtctcca cctccagcac tccacgatcg gcaaagaaac gacggatctc cgccataatc
     6541 gccgcgcgtt ttaataagtt aggaatggat gcgctcggct gccaggatgc cgtttcgctc
     6601 atagttaaat ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc gacggcgaga
     6661 caaattttac gcaggaatca aacagcggtg ggcagtgact aaaaaaagca cgatctgatg
     6721 gtttagtaat taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa
     6781 tcgatctcgt caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc
     6841 agtggaatag cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg
     6901 ggataaaaac aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag
     6961 gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa
     7021 aaatcgcact aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg
     7081 gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag
     7141 cgggtggcga ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa
     7201 ccgaaatgac ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg
     7261 tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac ctggttcgcc gccgataaga
     7321 ccggcttcca tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc
     7381 gttttgacga acatttcgtg ctggatattc tggttgatga tggtcatgtt cgcggcgtgg
     7441 tagcaatgaa catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg
     7501 ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc gttaccggtg
     7561 acggtatggg tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt
     7621 atcacccaac cggtctgcca ggttccggta tcctgatgac cgaaggctgc cgcggtgaag
     7681 gcggtattct ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg
     7741 aaactccgct gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt
     7801 ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg
     7861 tggtttatct cgacttgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca
     7921 tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg attccggtac
     7981 gtccgaccgc acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca
     8041 ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc
     8101 tgggttctaa ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga
     8161 cagagcgtgc agcaactgcc ggtaatggca acgaagcggc aattgaagcg caggcagctg
     8221 gcgttgaaca acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga
     8281 tccgcgacga aatgggcctg gcaatggaag aaggttgcgg tatctaccgt acgccggaac
     8341 tgatgcagaa aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca
     8401 tcaccgacac ctccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg
     8461 gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg
     8521 cacaccagcg tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca
     8581 ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga gtacagcgac gtgaagatta
     8641 ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg
     8701 aagcagccaa taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg
     8761 gtgcgctata acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat
     8821 gacgcaacta cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac
     8881 ctgagttacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac
     8941 aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt
     9001 gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac ccacttcatc
     9061 gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt
     9121 actaacatcc agaccccggc gcagatggca aagtatcacc agttctccgg ttgcatcaac
     9181 tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg
     9241 gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag
     9301 cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt gggctattgc
     9361 tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa
     9421 agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac
     9481 taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta
     9541 tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact
     9601 gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt
     9661 tttacaaaac ccggttatcg tgatcattaa cctgatcact ctggcggcag ctctgctgca
     9721 caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa
     9781 aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt
     9841 aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc
     9901 gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta
     9961 ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg
    10021 atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc
    10081 tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc
    10141 acgatctgaa aattcacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc
    10201 tgacagttgt cacgctgatt ggtatcgtta caatctaacg tatcgccaat gtaaatccgg
    10261 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
    10321 cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat
    10381 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
    10441 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
    10501 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
    10561 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa
    10621 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac
    10681 ctacactgct ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt
    10741 gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca ggaacacaac gtctgtatgc
    10801 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
    10861 gcaggcgatg caaacacgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
    10921 accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggcaagg cagttcatgt
    10981 ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag tcgaccattg aagatatggc
    11041 ctgctgggta cgaagcaata tgaatccccg tgatatcaac gacaaaacac ttcagcaagg
    11101 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg
    11161 ggaaatgctg gactggccgg taaatcctga cagcatcatt aacggcagtg gcaataaaat
    11221 tgcactggca gcacaccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc
    11281 atgggtacat aaaacagggg caaccggcgg atttggtagc tatgtcgcgt ttattccaga
    11341 aaaagagctg ggtatagtga tgctggctaa caaaaactat cccaatccag cgagagtcgc
    11401 cgccgcctgg cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga
    11461 ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat
    11521 ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca
    11581 gttctcctac gccgccgcgt ggcgtaacag tagtgaataa tttcgacgcc aaacgttatc
    11641 tcggtacctg gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag
    11701 tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata
    11761 accctgacag aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa
    11821 ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg
    11881 cactcgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga
    11941 tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga
    12001 cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag
    12061 tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg
    12121 ggttagcgga ctcaccgagc agcacaatgc cggtgatagc cgcgccgact gcgccaatac
    12181 ccgtccacac ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg
    12241 ccatactgac aatcatcgcc gtcacggtaa taacactcgg cgtcaaacga ctaaagccgt
    12301 gggtatattt caggccaacg gcccatacca cttccagcag accagcaata actaagataa
    12361 tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca
    12421 ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa
    12481 ctattacctt agtgactatc gcagaaaaaa caaacggcac gacatacctg tcgtaccgtt
    12541 attgcttatt gctattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca
    12601 gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc
    12661 agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg
    12721 ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa tgacggaaat
    12781 ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga
    12841 gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg
    12901 caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca
    12961 gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg
    13021 tatccacttt gatgacttcg ccgatttgta cgaacagcgg aaccttaacc acagcgccag
    13081 tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg
    13141 tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc
    13201 acagagttac gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt
    13261 ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt
    13321 cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag
    13381 atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa
    13441 atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt
    13501 ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct
    13561 ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc
    13621 ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt
    13681 ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag
    13741 ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac
    13801 gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc
    13861 tccaccgacc cgctggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac
    13921 aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt
    13981 cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac tgctcttgag
    14041 tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg
    14101 gcgaaagatc acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa
    14161 cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg
    14221 gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc
    14281 aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc
    14341 ctgcttaacc agagcgttct gttacgtggt gtgaacgata acgcacaaac gctggcaaac
    14401 ctgagtaatg cgttgttcga tgccggcgta atgccgtatt acctgcatgt gctcgataaa
    14461 gtacagggcg cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag
    14521 ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg cgcgagaaat cggcggcgaa
    14581 cccagcaaaa cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt
    14641 taaacattag cctgatgcgc tacgcttatc aggcctacat tgtgcattga aattgaatgg
    14701 atttgcaatg aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa
    14761 cgcaagtaca gcaacctgtc attaaactgg gaaatttaat ctttgcacgt tgcataacct
    14821 ttaattattc atatgcattg ctgttgttac tttttatata gctaacacca ataattaacc
    14881 tttcttttct gtttatatag agtgagcacg gctatttatg ttattttaac aactattact
    14941 accaagccca tttatctggc ttataaatta aaattaatta tttaattgag aaatggttag
    15001 ggagaaccta catggccata agcatcaagg gtgttaatac cggtgttatt cgcaagagta
    15061 ataatttcat tgcactggcg ctgaaaatta aagaacctcg caataaagaa tcgttgttct
    15121 ttatgtctgt catggaactg cgcgacctgc tgattgcgct ggaaagtcgt atgcatcaaa
    15181 agcataaact ggatgctgct gcacgccttc agtatgagca agctcgcgat aaagtcatta
    15241 aaaaaatggc agaaaacatc ccagaaattc tggttgatga gcttaaaaat gccgatatta
    15301 atcgtcgggt taacacgctg gaacttaccg ataatcaagg cgaaaatctt accttcgtat
    15361 taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc
    15421 tggcgcgagc aatcattcat gcaattaata atgcggaaat gcgtgaactg gcattacgta
    15481 ttacctcact gttagatttc ctgccgttat atgacgttga ctgccaggaa aatggcaatc
    15541 tggaatatga cacctattcc caaccggaat ggaaacataa cctgttcgac cattatctgg
    15601 cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc gcagtggtga
    15661 aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat cactcgccga atgcttgatt
    15721 tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag
    15781 cggcgaataa cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg catcacttac
    15841 gtgttcaatc caccagcaaa actgcaccac aggcgaaata atctcatctt tgctaatgcg
    15901 aggctggagc catccagcct gaaatgactg ataacgtgat cgttggtcat gccggatgcg
    15961 gcgtaaacgc cttatccggc ctacaa
//
LOCUS       DAZGCS010000535        15986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.110, whole genome shotgun sequence.
ACCESSION   DAZGCS010000535 DAZGCS010000000
VERSION     DAZGCS010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.110"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..408
                     /locus_tag="ACHK01_005675"
     CDS             <1..408
                     /locus_tag="ACHK01_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFO8100365.1"
                     /translation="LFDTEFGPMAQILVGATIVGSIETVWAGTITPPREGIIKRWTWP
                     AGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSVTKIGQPLAVSTET
                     FVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            430..3753
                     /gene="mscM"
                     /locus_tag="ACHK01_005676"
     CDS             430..3753
                     /gene="mscM"
                     /locus_tag="ACHK01_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8100366.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3904..5406)
                     /gene="yjeM"
                     /locus_tag="ACHK01_005677"
     CDS             complement(3904..5406)
                     /gene="yjeM"
                     /locus_tag="ACHK01_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8100367.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(5625..6602)
                     /gene="epmA"
                     /locus_tag="ACHK01_005678"
     CDS             complement(5625..6602)
                     /gene="epmA"
                     /locus_tag="ACHK01_005678"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8100368.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            6927..8735
                     /gene="frdA"
                     /locus_tag="ACHK01_005679"
     CDS             6927..8735
                     /gene="frdA"
                     /locus_tag="ACHK01_005679"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8100369.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8728..9462
                     /gene="frdB"
                     /locus_tag="ACHK01_005680"
     CDS             8728..9462
                     /gene="frdB"
                     /locus_tag="ACHK01_005680"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8100370.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            9473..9868
                     /gene="frdC"
                     /locus_tag="ACHK01_005681"
     CDS             9473..9868
                     /gene="frdC"
                     /locus_tag="ACHK01_005681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8100371.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            9879..10238
                     /gene="frdD"
                     /locus_tag="ACHK01_005682"
     CDS             9879..10238
                     /gene="frdD"
                     /locus_tag="ACHK01_005682"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8100372.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            10301..11434
                     /locus_tag="ACHK01_005683"
     CDS             10301..11434
                     /locus_tag="ACHK01_005683"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8100373.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFFPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            11523..12056
                     /gene="blc"
                     /locus_tag="ACHK01_005684"
     CDS             11523..12056
                     /gene="blc"
                     /locus_tag="ACHK01_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8100374.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(12053..12370)
                     /gene="sugE"
                     /locus_tag="ACHK01_005685"
     CDS             complement(12053..12370)
                     /gene="sugE"
                     /locus_tag="ACHK01_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8100375.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(12552..12698)
                     /gene="ecnB"
                     /locus_tag="ACHK01_005686"
     CDS             complement(12552..12698)
                     /gene="ecnB"
                     /locus_tag="ACHK01_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8100376.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(12809..12934)
                     /gene="ecnA"
                     /locus_tag="ACHK01_005687"
     CDS             complement(12809..12934)
                     /gene="ecnA"
                     /locus_tag="ACHK01_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8100377.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(12986..13552)
                     /gene="efp"
                     /locus_tag="ACHK01_005688"
     CDS             complement(12986..13552)
                     /gene="efp"
                     /locus_tag="ACHK01_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8100378.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            13594..14622
                     /gene="epmB"
                     /locus_tag="ACHK01_005689"
     CDS             13594..14622
                     /gene="epmB"
                     /locus_tag="ACHK01_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8100379.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            15012..15881
                     /locus_tag="ACHK01_005690"
     CDS             15012..15881
                     /locus_tag="ACHK01_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8100380.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4059 a         4140 c         4112 g         3675 t
ORIGIN      
        1 ctgttcgata ccgaatttgg cccgatggcg cagattctgg tcggggcgac gattgtaggc
       61 agcattgaga cggtctgggc gggcaccatt acgccgccgc gcgaaggtat catcaagcgc
      121 tggacctggc ctgccgggga aaacgacggt tctgtggcac tgctgaaagg ccaggaaatg
      181 ggccgcttta aactcggctc taccgttatc aacctgtttg caccgggtaa agtgaatctg
      241 gttgagcaac tggaaagcct gtctgtcacg aaaattggcc agccgctggc agtatctacc
      301 gaaacctttg ttacgccaga cgctgaaccc gccccacttc ctgctgaaga gatcgaagca
      361 gaacacgacg ccagcccatt ggttgacgat aaaaaagacc aggtctaatc catcaaagga
      421 aacgctgacg tgcgcctgat tatcactttt ctgatggcct ggtgcctcag ttggggggcg
      481 tacgccgcga cggcccccga tagcaaacaa atcactcagg aactggagca ggcaaaagcg
      541 gcgaaacccg cacagccgga agtcgtagag gcgctccagt ctgccttaaa tgcgcttgag
      601 gaacgaaaag gttcccttga gcgcatcaaa caatatcagc aagttatcga taattatccg
      661 aaactctccg ctactctgcg cgcacaatta aacaacatgc gtgacgagcc gcgcagcgtg
      721 tcgccgggaa tgtctaccga cgcgctgaat caggaaattc tccaggtcag cagccagttg
      781 ctggataaaa gccgtcaggc ccagcaagag caggagcgcg cccgcgagat tgccgattcg
      841 ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc agttaaatga gatcgagcgc
      901 cgcctgggaa cgcttactgg caataccccg ctcaatcagg cacaaaattt cgcgttgcag
      961 tctgactccg cacgcctgaa agcgctcgtt gatgaactgg aactggcgca gctgtctgcc
     1021 aataaccgtc aggaattagc gcgcttacgc tcagagctgg cggaaaaaga gagccagcaa
     1081 ctggatgcgt atttgcaggc cttacgtaat cagttgaaca gccaacgtca gcttgaggcg
     1141 gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca gcgccgattt gccgaaagac
     1201 atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg cactgaatca acaggcgcag
     1261 cggatggatc tcgttgcctc gcaacagcgc caggccacca gccagacgtt acaggttcgg
     1321 caggcgctga atacgctgcg tgaacagtcg caatggctgg gatcgtccaa cctgctcggc
     1381 gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc cgaaaccaca acagcttgat
     1441 accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg aggatctgct taataaacag
     1501 ccgctgctac ggcaaattca tcaggccgac ggtcagccgc tgaccgccga gcaaaaccgt
     1561 attctggaag cacaactgcg cactcagcgt gagttgctga actcattgtt gcagggtggc
     1621 gacacgctac tactggaact gaccaagctg aaagtctcca acgggcaact ggaggatgcg
     1681 ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct ggacctctga cgtgcgcccg
     1741 atgaccatcg cctggccgct ggaaatcgcc caggatctgc gtcgtctcat ttcgctggac
     1801 accttcagtc agttgggcaa agccagtgtg atgatgctga ccagcaaaga gacgattttg
     1861 ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt actcgcgccg ctatttcacc
     1921 cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga ctcaggatca cttctggctg
     1981 acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac cgctgccggt gctgtggatg
     2041 acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc tggcggtcgc gattggcgat
     2101 ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga tttgcgccac ctttgcccgc
     2161 ccgaacggct tgtttatcgc tcattttggc tggccgcgcg aacgtgtttc ccgtgggatg
     2221 cgctactacc tgatgagcat cgggcttatt gtgccgctga ttatggcgct gatgatgttc
     2281 gataacctcg acgaccgtga attctccggt tcgctgggac ggctttgctt tatcctcatt
     2341 tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg ccgggatccc gctgtacctc
     2401 aacaaagagg gcagcggcga caacattacc aaccatatgc tgtggaacat gatgattggc
     2461 gcaccactgg ttgccattct ggcgtcagcg gtgggttatc tggcaacggc gcaggcactg
     2521 ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc tactggtggt ttatcacgtt
     2581 atccgccgct ggatgctgat ccagcgtcgc aggctggcgt ttgaccgggc gaagcatcgc
     2641 cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg aagcgcatca tcacagtagc
     2701 ccggaagggg caattgaagt cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcaa
     2761 tccttgcggc tggtgcgctc aattttgatg ttgatcgccc tgctttctgt cattgtgctg
     2821 tggtcagaaa tccattccgc tttcggcttc ctcgaaaata tttcgctgtg ggatgtcacc
     2881 tccacggtac agggcgtaga aagtctggag ccaattaccc tcggtgcggt gctgattgcc
     2941 attctggtgt ttatcatcac cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg
     3001 gcgattttgc agcacctgga tttaacgccg ggtacgggct acgccatcac caccatcacc
     3061 aaatatctgc tgatgctgat tggcgggctg gtcggcttct cgatgattgg tattgagtgg
     3121 tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc tcggttttgg tttgcaggag
     3181 attttcgcca actttatctc tggcctgatt atcctgttcg aaaaaccgat tcgcattggc
     3241 gatacggtaa cgatccgcga tcttaccggt agcgtgacga aaattaacac ccgcgccacc
     3301 accatcagcg actgggaccg taaagagata atcgtgccga acaaggcgtt tattaccgag
     3361 cagtttatca actggtcgct ctctgactcg gtcacgcgcg tggtgttgac gataccggcc
     3421 cctgccgatg ccaatagcga agaagtgacg gaaatcctgc tcaccgcagc gcgtcgctgc
     3481 tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg tggatctgca acaggggatt
     3541 cagattttcg agctgcgtat ttacgccgct gagatgggtc accgtatgcc gatacgccat
     3601 gagatccacc agctgattct ggcaggcttc cacgcccacg gtatcgatat gccattcccg
     3661 cccttccaga tgcgtctgga aagcctcaac ggtaagcaaa cggggagaac attaacttct
     3721 gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga agctgccaaa tgatgtcaac
     3781 gtggctcatt tagcagctta acatcatctt cagttatcac cggccctgga gagtatcatc
     3841 catacgcatt acttcctgac aactcatcgc cggagagacg atgagttgca ggatttcata
     3901 tgatcagtcg agagctaact caggtttatt cgccatgcga ctgcaatagt tctggtaaat
     3961 cgccatcgct aacagcgaga agaagacagg gccgccaatc atccacaatg tgctgtccca
     4021 gtctccggct tcaaccacag gttgaatgat ggtgaagacg ttcgcaaatg tcaccaccag
     4081 tacgacaacc accgtcgcaa tcattgccga catacgcgtt ttgaaaatca caaatggtct
     4141 gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc gcgaggaaca ggtaaggaag
     4201 cgtcatagac acgttcgcca tcagcgtcag cttgttaaag aacgccgatg cggtaccgcc
     4261 accaaacgaa accagcagga tgaagacagt aaccaacccg cactgcatcc acatggcgat
     4321 agaaggcatc cccatcgcat tcaggcgcgt catcggttcc ggccacaacg ctttcggcgt
     4381 cccctggatg atggctttca gcggtgaata gcaaagcgta aagaacgccc cggtataagc
     4441 gaggaacatc gaaagtccgg taatacgcgc aaaccataca cccagcgaca atgacgcttc
     4501 aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg ctcttcatca gcacataggt
     4561 aatattgccg aggttaacgg aaccattact taatacctgc tgccagtttg tgctgacacc
     4621 ccataaaaat attgccagcg aataaccgat tgaaataaca atagcggcaa aaacaatacc
     4681 tttggcaaag ttcttttctg gattttccgt tttatcgacc agaccaccaa ccgcttcaat
     4741 tccgccatag gcaaaaatag caaataccac aaacgatagc attgccagac cggactgata
     4801 acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg aaatgcccac catttaataa
     4861 caaaatagta atgcttacta acagcaatac taaattcaga cacattactg caataccgcc
     4921 caccgcagta atgcgggcaa ttttattaat cccctttgaa gcaacgacgg tgaccagaat
     4981 catccatgct actgccagca gaccaaccac ctgcgtaggc tccagtccgg caatacgcca
     5041 gtgctgggtc atgtcgctac catagaggaa tgttgagaac ggtacccaaa ctttcgcgga
     5101 ggtactcacc atccagatga tataagagga aaaccacata aacgtaccaa tgaaggcaaa
     5161 acgtggtccg acactattat tcatccagga atagatacca ccttcttctt tgcgataagc
     5221 agctcccatt tcagccatca ttaaggcgaa tggaataaag aataacaatg cagaaaatat
     5281 ataaaaggga atcgcactat aacccattaa gtaataagcc gatgggctat tggcaaatcc
     5341 aaatacggaa gtaaagatca tcaatatgag tcctatcaga ctcatctttt ttatcgtgtg
     5401 aggcatgaaa ccatccttca tttcagcgcg tgtataacac gtgcccactg aactgcactg
     5461 cgcaacattg agcaggcagc gtaaaaacca atacagaatt gatacctgga tgatatcaaa
     5521 tatccagtct taaattgtgg cctttaacgg aaaaatgaaa acatatgcta cagaatggcg
     5581 cttatcacgc cattcttcgc tgttaattca gtaatttttc agaattatgc ccggtcaacg
     5641 ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg ccaacatcac cagacgatca
     5701 acacctaatg ccacgccgga acagtcaggc ataccgactt tcaaggcttc aatcagattc
     5761 tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct tacggttatc ttgttcaaag
     5821 cgttgttgct gctcacgggc atccgtcaat tcatggaaac cattcgccag ctcaatacct
     5881 ttataataaa cctcaaagcg ttcagcgacc cgatgatctt cggtactgat ttgcgccagt
     5941 gatgcctggc tggctggaaa gtggtacaca aaggtcggtt tttctttgcc aatatttggc
     6001 tctacgccaa aggtaaacag caattgtagc agcgtgtcgc ggtcttcttc ggtatcagca
     6061 acattgctca aatccagttt cgctgcgact tcccgcagtt gcgttttgtc ggcagagagc
     6121 gggtcaattt ccagataacg caagaaagct tgttgataag aaaggctttc tgctgccggg
     6181 cagtccagca cctgttgtaa gagatcgtcc acctcgttca tcaaccggta catatcatag
     6241 tgcggtcgat accactccag catagtgaac tcagggttgt gataacgccc catctcttca
     6301 ttacggaagc tgcggcacag ctggaatacc ggcccacaac cggcaaccag caggcgtttc
     6361 atatggtatt ccgggctggt cattaaccag agattcatcc cctgcgaatg cccggggcca
     6421 acgaaacgtg tctcaaacgg gaccaaatga atatcggtta ccgtcgcctg gctcatacaa
     6481 ggcgtctcca cctccagcac tccacgatcg gcaaagaaac gacggatctc cgccataatc
     6541 gccgcgcgtt ttaataagtt aggaatggat gcgctcggct gccaggatgc cgtttcgctc
     6601 atagttaaat ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc gacggcgaga
     6661 caaattttac gcaggaatca aacagcggtg ggcagtgact aaaaaaagca cgatctgatg
     6721 gtttagtaat taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa
     6781 tcgatctcgt caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc
     6841 agtggaatag cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg
     6901 ggataaaaac aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag
     6961 gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa
     7021 aaatcgcact aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg
     7081 gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag
     7141 cgggtggcga ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa
     7201 ccgaaatgac ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg
     7261 tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac ctggttcgcc gccgataaga
     7321 ccggcttcca tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc
     7381 gttttgacga acatttcgtg ctggatattc tggttgatga tggtcatgtt cgcggcgtgg
     7441 tagcaatgaa catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg
     7501 ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc gttaccggtg
     7561 acggtatggg tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt
     7621 atcacccaac cggtctgcca ggttccggta tcctgatgac cgaaggctgc cgcggtgaag
     7681 gcggtattct ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg
     7741 aaactccgct gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt
     7801 ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg
     7861 tggtttatct cgacttgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca
     7921 tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg attccggtac
     7981 gtccgaccgc acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca
     8041 ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc
     8101 tgggttctaa ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga
     8161 cagagcgtgc agcaactgcc ggtaatggca acgaagcggc aattgaagcg caggcagctg
     8221 gcgttgaaca acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga
     8281 tccgcgacga aatgggcctg gcaatggaag aaggttgcgg tatctaccgt acgccggaac
     8341 tgatgcagaa aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca
     8401 tcaccgacac ctccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg
     8461 gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg
     8521 cacaccagcg tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca
     8581 ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga gtacagcgac gtgaagatta
     8641 ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg
     8701 aagcagccaa taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg
     8761 gtgcgctata acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat
     8821 gacgcaacta cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac
     8881 ctgagttacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac
     8941 aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt
     9001 gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac ccacttcatc
     9061 gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt
     9121 actaacatcc agaccccggc gcagatggca aagtatcacc agttctccgg ttgcatcaac
     9181 tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg
     9241 gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag
     9301 cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt gggctattgc
     9361 tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa
     9421 agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac
     9481 taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta
     9541 tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact
     9601 gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt
     9661 tttacaaaac ccggttatcg tgatcattaa cctgatcact ctggcggcag ctctgctgca
     9721 caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa
     9781 aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt
     9841 aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc
     9901 gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta
     9961 ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg
    10021 atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc
    10081 tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc
    10141 acgatctgaa aattcacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc
    10201 tgacagttgt cacgctgatt ggtatcgtta caatctaacg tatcgccaat gtaaatccgg
    10261 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
    10321 cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat
    10381 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
    10441 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
    10501 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
    10561 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa
    10621 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac
    10681 ctacactgct ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt
    10741 gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca ggaacacaac gtctgtatgc
    10801 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
    10861 gcaggcgatg caaacacgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
    10921 accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggcaagg cagttcatgt
    10981 ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag tcgaccattg aagatatggc
    11041 ctgctgggta cgaagcaata tgaatccccg tgatatcaac gacaaaacac ttcagcaagg
    11101 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg
    11161 ggaaatgctg gactggccgg taaatcctga cagcatcatt aacggcagtg gcaataaaat
    11221 tgcactggca gcacaccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc
    11281 atgggtacat aaaacagggg caaccggcgg atttggtagc tatgtcgcgt tttttccaga
    11341 aaaagagctg ggtatagtga tgctggctaa caaaaactat cccaatccag cgagagtcgc
    11401 cgccgcctgg cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga
    11461 ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat
    11521 ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca
    11581 gttctcctac gccgccgcgt ggcgtaacag tagtgaataa tttcgacgcc aaacgttatc
    11641 tcggtacctg gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag
    11701 tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata
    11761 accctgacag aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa
    11821 ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg
    11881 cactcgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga
    11941 tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga
    12001 cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag
    12061 tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg
    12121 ggttagcgga ctcaccgagc agcacaatgc cggtgatagc cgcgccgact gcgccaatac
    12181 ccgtccacac ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg
    12241 ccatactgac aatcatcgcc gtcacggtaa taacactcgg cgtcaaacga ctaaagccgt
    12301 gggtatattt caggccaacg gcccatacca cttccagcag accagcaata actaagataa
    12361 tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca
    12421 ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa
    12481 ctattacctt agtgactatc gcagaaaaaa caaacggcac gacatacctg tcgtaccgtt
    12541 attgcttatt gctattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca
    12601 gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc
    12661 agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg
    12721 ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa tgacggaaat
    12781 ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga
    12841 gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg
    12901 caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca
    12961 gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg
    13021 tatccacttt gatgacttcg ccgatttgta cgaacagcgg aaccttaacc acagcgccag
    13081 tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg
    13141 tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc
    13201 acagagttac gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt
    13261 ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt
    13321 cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag
    13381 atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa
    13441 atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt
    13501 ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct
    13561 ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc
    13621 ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt
    13681 ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag
    13741 ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac
    13801 gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc
    13861 tccaccgacc cgctggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac
    13921 aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt
    13981 cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac tgctcttgag
    14041 tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg
    14101 gcgaaagatc acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa
    14161 cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg
    14221 gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc
    14281 aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc
    14341 ctgcttaacc agagcgttct gttacgtggt gtgaacgata acgcacaaac gctggcaaac
    14401 ctgagtaatg cgttgttcga tgccggcgta atgccgtatt acctgcatgt gctcgataaa
    14461 gtacagggcg cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag
    14521 ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg cgcgagaaat cggcggcgaa
    14581 cccagcaaaa cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt
    14641 taaacattag cctgatgcgc tacgcttatc aggcctacat tgtgcattga aattgaatgg
    14701 atttgcaatg aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa
    14761 cgcaagtaca gcaacctgtc attaaactgg gaaatttaat ctttgcacgt tgcataacct
    14821 ttaattattc atatgcattg ctgttgttac tttttatata gctaacacca ataattaacc
    14881 tttcttttct gtttatatag agtgagcacg gctatttatg ttattttaac aactattact
    14941 accaagccca tttatctggc ttataaatta aaattaatta tttaattgag aaatggttag
    15001 ggagaaccta catggccata agcatcaagg gtgttaatac cggtgttatt cgcaagagta
    15061 ataatttcat tgcactggcg ctgaaaatta aagaacctcg caataaagaa tcgttgttct
    15121 ttatgtctgt catggaactg cgcgacctgc tgattgcgct ggaaagtcgt atgcatcaaa
    15181 agcataaact ggatgctgct gcacgccttc agtatgagca agctcgcgat aaagtcatta
    15241 aaaaaatggc agaaaacatc ccagaaattc tggttgatga gcttaaaaat gccgatatta
    15301 atcgtcgggt taacacgctg gaacttaccg ataatcaagg cgaaaatctt accttcgtat
    15361 taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc
    15421 tggcgcgagc aatcattcat gcaattaata atgcggaaat gcgtgaactg gcattacgta
    15481 ttacctcact gttagatttc ctgccgttat atgacgttga ctgccaggaa aatggcaatc
    15541 tggaatatga cacctattcc caaccggaat ggaaacataa cctgttcgac cattatctgg
    15601 cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc gcagtggtga
    15661 aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat cactcgccga atgcttgatt
    15721 tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag
    15781 cggcgaataa cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg catcacttac
    15841 gtgttcaatc caccagcaaa actgcaccac aggcgaaata atctcatctt tgctaatgcg
    15901 aggctggagc catccagcct gaaatgactg ataacgtgat cgttggtcat gccggatgcg
    15961 gcgtaaacgc cttatccggc ctacaa
//
LOCUS       DAZGCS010000536         8382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.163, whole genome shotgun sequence.
ACCESSION   DAZGCS010000536 DAZGCS010000000
VERSION     DAZGCS010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.163"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..626)
                     /locus_tag="ACHK01_005691"
     CDS             complement(<1..626)
                     /locus_tag="ACHK01_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFO8100381.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTL"
     gene            887..1345
                     /gene="gpt"
                     /locus_tag="ACHK01_005692"
     CDS             887..1345
                     /gene="gpt"
                     /locus_tag="ACHK01_005692"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8100382.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1437..2681
                     /gene="frsA"
                     /locus_tag="ACHK01_005693"
     CDS             1437..2681
                     /gene="frsA"
                     /locus_tag="ACHK01_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8100383.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2739..3140
                     /gene="crl"
                     /locus_tag="ACHK01_005694"
     CDS             2739..3140
                     /gene="crl"
                     /locus_tag="ACHK01_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8100384.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3179..4234)
                     /gene="phoE"
                     /locus_tag="ACHK01_005695"
     CDS             complement(3179..4234)
                     /gene="phoE"
                     /locus_tag="ACHK01_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8100385.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            4522..5625
                     /gene="proB"
                     /locus_tag="ACHK01_005696"
     CDS             4522..5625
                     /gene="proB"
                     /locus_tag="ACHK01_005696"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO8100386.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            5637..6890
                     /gene="proA"
                     /locus_tag="ACHK01_005697"
     CDS             5637..6890
                     /gene="proA"
                     /locus_tag="ACHK01_005697"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO8100387.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            7005..7080
                     /locus_tag="ACHK01_005698"
     tRNA            7005..7080
                     /locus_tag="ACHK01_005698"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7038..7040,aa:Thr,seq:cgt)
     gene            complement(7207..7545)
                     /locus_tag="ACHK01_005699"
                     /pseudo
     CDS             complement(7207..7545)
                     /locus_tag="ACHK01_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(7596..>8382)
                     /gene="paoD"
                     /locus_tag="ACHK01_005700"
     CDS             complement(7596..>8382)
                     /gene="paoD"
                     /locus_tag="ACHK01_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO8100388.1"
                     /translation="GAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYG
                     EGSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPT
                     QTRTGWNLNGFEVGFRPCVRLMIYGRSLETQATASLAAATGYDSHIFDLFPASASAQI
                     DTDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETT
                     QIRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
BASE COUNT         2020 a         2122 c         2205 g         2035 t
ORIGIN      
        1 tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg
       61 ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag
      121 tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc
      181 ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg
      241 tcagccagaa ctgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg
      301 ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca
      361 tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt
      421 ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg
      481 cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct
      541 tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt
      601 ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt
      661 attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg
      721 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
      781 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
      841 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
      901 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
      961 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt
     1021 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     1081 cgacaaccag cgcgagctta aagtgctgaa acgtgcagaa ggcgatggcg aaggcttcat
     1141 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     1201 aaaagcgcac tttgtcacca tcttcgcaaa accggccggt cgcccgctgg ttgatgatta
     1261 tgttgttgat atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt
     1321 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     1381 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     1441 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     1501 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta
     1561 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg
     1621 acccacgtga aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg
     1681 acgatgattt atacgatact gtgattggct accgtggtgg caactggatt tatgagtggg
     1741 ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg
     1801 gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag
     1861 gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc
     1921 agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca
     1981 ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg
     2041 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     2101 gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     2161 cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg
     2221 tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     2281 tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata
     2341 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     2401 cgagtcgttt ggggatgcat gatgcttccg atgacgcgtt gcgcgtggag ctgaatcgct
     2461 attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct
     2521 actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg
     2581 ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc
     2641 ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc
     2701 aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc
     2761 cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt
     2821 gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac
     2881 cggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta
     2941 cctacagcta ccagtttggt ctgttcgata aagcaggcga ctggaagagc gttccggtaa
     3001 aagacactga agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc
     3061 gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgacgagc
     3121 cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aataatcatt
     3181 aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc aatttgttat
     3241 cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat
     3301 atgtagcccc gacatcgata taattgacca gatcttcatc accgatacct tcaatatctt
     3361 tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag
     3421 cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc
     3481 gtgtttcaga ataaaaagtt gccagataaa tattattggc atcgtatttc agacctgttg
     3541 cccatgcttc tgcacgcttg ccagtgccac ggctttgcag gttctgctcg ttggtgcgat
     3601 ctgagttggt ataggcacca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg
     3661 acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat
     3721 attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg
     3781 ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag tcgccaccaa
     3841 attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacggccat
     3901 agtcgaaaga acccaaatct ttatacttca atccggcaaa agcgagacgc gttttttgct
     3961 gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaacgg ccatagccag
     4021 tcagttgatc gttaatttgt gtttcgcctt taaaaccaaa acggatataa ctctggtcgc
     4081 catctttact gtcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca
     4141 gtttattacc gtctttgtta tatatttctg cggcctgtac ggatgcagat gccacaatgc
     4201 ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg
     4261 cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa
     4321 agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat ctgtaataaa
     4381 agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca tgctttgctt
     4441 gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct
     4501 aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc
     4561 agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atatcgttga acttgttcgc
     4621 cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc
     4681 gccgccggac gtgagcacct ggggtacccg gaactgccag cgactatcgc ctcgaaacaa
     4741 ctgctggcgg cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt
     4801 tatggcattc acgtcgggca gatgctgctg acccgtgctg atatggaaga ccgtgaacgc
     4861 ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc
     4921 aatgagaacg atgctgtcgc tacggcagag attaaggtcg gcgataacga taacctttct
     4981 gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt
     5041 ttgtataccg ctgatccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc
     5101 attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg aactggcggc
     5161 atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga caccattatt
     5221 gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg
     5281 ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg
     5341 ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattcttga acgcggcagc
     5401 tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg tgaagtcatc
     5461 cgcatttgca acctcgaagg tcgcgatatc gcccacggcg tcagtcgtta caacagcgat
     5521 gcattacgcc gtattgccgg gcaccactcg caagaaattg atgcaatact gggatatgaa
     5581 tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc aggctgatgc
     5641 tggaacaaat gggcattgcc gcgaagcaag cctcgtataa attagcgcaa ctctccagcc
     5701 gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa agcgaaatca
     5761 tcctcaacgc taacgcccag gatgttgctg acgcgcgtgc caatggcctt ggcgaagcga
     5821 tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgat gatgtgcgcc
     5881 aggtgtgtaa cctcgccgat ccggtggggc aggtaatcga tggcagcgta ctggacagcg
     5941 gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt tatgaagcgc
     6001 gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat gcggtgatcc
     6061 tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg attcaggacg
     6121 ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat cctgaccgtg
     6181 cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc ccgcgtggtg
     6241 gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc acaggtggta
     6301 taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca ttaaaagtga
     6361 tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg ttgctggtga
     6421 ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg gaaagcggcg
     6481 tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct gcgaaggtgg
     6541 ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg aacgtcaaaa
     6601 tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca caacactccg
     6661 atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa gtggattcgt
     6721 ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt ggtctgggtg
     6781 cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg ctggaagcac
     6841 tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa ataaaaccgg
     6901 gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg gttagtttta
     6961 accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat atagctcagt
     7021 tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca
     7081 ttaaaatcaa agagttaccc catatttaaa tacaccacgt ttcctcctgt gccgcatttg
     7141 tgccattgta accttggcaa ttcatcaaaa tactgttctg acatcaggca gtgcaggtgc
     7201 agctatttaa accaattgct gccgccattc tttgacgtag tcaatcaggg cgcggagctt
     7261 tggtgcaata ttgcgacgct gtgggaaata cagatagaag cccggaaatt gtggaagaaa
     7321 gtcatcaagc agcgatacaa gcttaccgct ttcaatatat ggcctgaaag tttcctgagt
     7381 ggcaattgtt attcctccgc cggcaagagc cagcctcaac atcagacgca gatcattagt
     7441 cgtaatctgc ggttcaatcg caaggtcgaa agttctcccg ttttcttcaa atggccagcg
     7501 ataaggcgca acctccgggg actgacgcca gccgatacac ttatgggtat ttcccccgga
     7561 ggcgagaaag cgctctccac gcccggccgc aaggatcagg acgacggggg caggcatgaa
     7621 tcctcctcct gatggagacg tacagaggcg acttctgcca gcacggagag tgccagagta
     7681 tgcgcatccc gggctttggg gaatatcccg acgggtgccc ggatttgcgt tgtttcctcc
     7741 ctggaccatc ccagctcgtg gagcttttgc agacgtaacg tgtgggttcg atagctgccc
     7801 aatgcgccga gataaaaggg ttttgcttct cgcgcggcct gcaacactgg cagctcccgg
     7861 ttgagatcat ggcacagcaa aatgaccgcc gtatcggtat cgatctgagc gctggctgag
     7921 gccggaaaaa gatcgaagat atggctgtca tagcctgtgg ctgctgcaag actcgcagtt
     7981 gcctgcgtct caagagaacg tccgtaaatc atcagcctga cgcatggcct gaaccccacc
     8041 tcaaagccat tgagattcca gcccgttcgg gtttgcgtgg gcaggcacac cagcgattgt
     8101 gcttgcggat cgtagcgcag ccccgccggt tttctctgtt ccagacggtt cagcacggcg
     8161 agcagaggct gtgccgagcg tagtttatgg agcgtcagcg tgatcccacc gccgcagggc
     8221 agaacgatgt caaaccacgg cgaaccttcg ccatagcgaa tttcgcgatc gcggcctgag
     8281 cccatcatct ccagcgcttc aaaagcggca gcggcctcca cgcagccgcc agagacaaaa
     8341 ccgcagtaac gaccatcttc gcgaaccacc atctgcgccc cg
//
LOCUS       DAZGCS010000537         3085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.256, whole genome shotgun sequence.
ACCESSION   DAZGCS010000537 DAZGCS010000000
VERSION     DAZGCS010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.256"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(190..840)
                     /gene="tetR(A)"
                     /locus_tag="ACHK01_005701"
     CDS             complement(190..840)
                     /gene="tetR(A)"
                     /locus_tag="ACHK01_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO8100389.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            946..2145
                     /gene="tet(A)"
                     /locus_tag="ACHK01_005702"
     CDS             946..2145
                     /gene="tet(A)"
                     /locus_tag="ACHK01_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO8100390.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2177..3037)
                     /locus_tag="ACHK01_005703"
     CDS             complement(2177..3037)
                     /locus_tag="ACHK01_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO8100391.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          579 a          963 c          955 g          588 t
ORIGIN      
        1 cgccgcagca ggagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga
       61 cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga
      121 aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag
      181 cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct
      241 tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc
      301 ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt
      361 gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt
      421 gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca
      481 gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc
      541 atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc
      601 gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc
      661 cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag
      721 cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc
      781 gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat
      841 gcttgacact ttatcactga taaacataat atgtccacca acttatcagt gataaagaat
      901 ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacaga
      961 cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg
     1021 ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat
     1081 ggcattctgc tggcgctgta tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg
     1141 ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc
     1201 gactacgcca tcatggcgac ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg
     1261 gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat
     1321 ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc
     1381 gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc
     1441 gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac
     1501 aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg
     1561 gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc
     1621 ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg
     1681 accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg
     1741 atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt
     1801 gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg
     1861 atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc
     1921 aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc
     1981 ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg
     2041 tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg
     2101 cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag
     2161 gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt
     2221 tggaacgttg gcccagccag atactcccga tcacgagcag gacgccgatg atttgaagcg
     2281 cactcagcgt ctgatccaag aacaaccatc ctagcaacac ggcggtcccc gggctgagaa
     2341 agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt
     2401 aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag
     2461 gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg
     2521 ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca
     2581 cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta
     2641 gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca
     2701 ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca
     2761 gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa
     2821 acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc
     2881 agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc
     2941 gcagcatcgc gaccgtcatc ggtgagaagt tcggcaggta ttgggtggtg acaatgtagg
     3001 tgctgcccca aatggcaggt gctatcgctg tgaacaataa atcggggcag acggcgcttc
     3061 cagcgtgacg accatgccga gcacg
//
LOCUS       DAZGCS010000538         2894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.261, whole genome shotgun sequence.
ACCESSION   DAZGCS010000538 DAZGCS010000000
VERSION     DAZGCS010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.261"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            332..1147
                     /gene="sul2"
                     /locus_tag="ACHK01_005704"
     CDS             332..1147
                     /gene="sul2"
                     /locus_tag="ACHK01_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO8100392.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1208..2011
                     /locus_tag="ACHK01_005705"
                     /note="aph(3'')-Ib"
     CDS             1208..2011
                     /locus_tag="ACHK01_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO8100393.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2011..2847
                     /locus_tag="ACHK01_005706"
     CDS             2011..2847
                     /locus_tag="ACHK01_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO8100394.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          582 a          801 c          816 g          695 t
ORIGIN      
        1 cccggcccaa ggcggcaggc tgaccccccc ccactctatt gtaaacaaga catttttatc
       61 ttttatattc aatggcttat tttcctgcta attggtaata ccatgaaaaa taccatgctc
      121 agaaaaggct taacaatatt ttgaaaaatt gcctactgag cgctgccgca cagctccata
      181 ggccgctttc ctggctttgc ttccagatgt atgctcttct gctcctgcag ctaatggatc
      241 accgcaaaca ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgatactc
      301 atgctcgatt attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat
      361 cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc
      421 cattgcgcag gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc
      481 atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc
      541 ggtgctggac gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc
      601 gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc
      661 agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc
      721 ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat
      781 tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg
      841 ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc
      901 ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt
      961 ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc
     1021 gacactcgct gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga
     1081 gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat
     1141 tcgttaactg cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga
     1201 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1261 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1321 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1381 caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc
     1441 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     1501 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     1561 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     1621 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     1681 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     1741 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     1801 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     1861 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     1921 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     1981 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
     2041 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt
     2101 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     2161 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     2221 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     2281 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     2341 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     2401 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc
     2461 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     2521 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     2581 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     2641 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     2701 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc
     2761 gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc
     2821 aagcaggtgc gacagacgtc atactagata tcaagggcac tgttgcaaag ttagcgatga
     2881 ggcagccttt tgtc
//
LOCUS       DAZGCS010000539         1728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.317, whole genome shotgun sequence.
ACCESSION   DAZGCS010000539 DAZGCS010000000
VERSION     DAZGCS010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.317"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..984
                     /gene="stxA1a"
                     /locus_tag="ACHK01_005707"
     CDS             37..984
                     /gene="stxA1a"
                     /locus_tag="ACHK01_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO8100395.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            994..1263
                     /gene="stxB1a"
                     /locus_tag="ACHK01_005708"
     CDS             994..1263
                     /gene="stxB1a"
                     /locus_tag="ACHK01_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO8100396.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          460 a          312 c          428 g          528 t
ORIGIN      
        1 tagttatcgt atggtgctca aggagtattg tgtaatatga aaataattat ttttagagtg
       61 ctaacttttt tctttgttat cttttcagtt aatgtggtgg cgaaggaatt taccttagac
      121 ttctcgactg caaagacgta tgtagattcg ctgaatgtca ttcgctctgc aataggtact
      181 ccattacaga ctatttcatc aggaggtacg tctttactga tgattgatag tggcacaggg
      241 gataatttgt ttgcagttga tgtcagaggg atagatccag aggaagggcg gtttaataat
      301 ctacggctta ttgttgaacg aaataattta tatgtgacag gatttgttaa caggacaaat
      361 aatgtttttt atcgctttgc tgatttttca catgttacct ttccaggtac aacagcggtt
      421 acattgtctg gtgacagtag ctataccacg ttacagcgtg ttgcagggat cagtcgtacg
      481 gggatgcaga taaatcgcca ttcgttgact acttcttatc tggatttaat gtcgcatagt
      541 ggaacctcac tgacgcagtc tgtggcaaga gcgatgttac ggtttgttac tgtgacagct
      601 gaagctttac gttttcggca aatacagagg ggatttcgta caacactgga tgatctcagt
      661 gggcgttctt atgtaatgac tgctgaagat gttgatctta cattgaactg gggaaggttg
      721 agtagcgtcc tgcctgacta tcatggacaa gactctgttc gtgtaggaag aatttctttt
      781 ggaagcatta atgcaattct gggaagcgtg gcattaatac tgaattgtca tcatcatgca
      841 tcgcgagttg ccagaatggc atctgatgag tttccttcta tgtgtccggc agatggaaga
      901 gtccgtggga ttacgcacaa taaaatattg tgggattcat ccactctggg ggcaattctg
      961 atgcgcagaa ctattagcag ttgagggggt aaaatgaaaa aaacattatt aatagctgca
     1021 tcgctttcat ttttttcagc aagtgcgctg gcgacgcctg attgtgtaac tggaaaggtg
     1081 gagtatacaa aatataatga tgacgatacc tttacagtta aagtgggtga taaagaatta
     1141 tttaccaaca gatggaatct tcagtctctt cttctcagtg cgcaaattac ggggatgact
     1201 gtaaccatta aaactaatgc ctgtcataat ggagggggat tcagcgaagt tatttttcgt
     1261 tgactcagaa tagctcagtg aaaatagcag gcggagattc ataaatgtta aatacatctc
     1321 aattcagtca gttgttgccg gtctgataat agatgtgtta gaaaatttct gcatggtgaa
     1381 tccccctgtg cggaggggcg actggtgaac ggtatgatct ctttgatgat cgtaagcgag
     1441 aatacgcggg tttggtggca ccaggccgaa ctcaccggga ggcacccggc atcatgctgt
     1501 atacagagat taggcatata tccaggctcc tcatcgcagg agccttttta catgcaaaaa
     1561 aaagcccgag tgggttcggg caacagcatg agatacttgc attgtcattt ttatcgtgcg
     1621 gattttaacc aggattcata aggctgcgca actgcgcggc ctttttcgta tttcgggctg
     1681 tagtccctgt gtgtcattca ggcttccgga ctacagccca ctccatat
//
LOCUS       DAZGCS010000540         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.319, whole genome shotgun sequence.
ACCESSION   DAZGCS010000540 DAZGCS010000000
VERSION     DAZGCS010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.319"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..379
                     /locus_tag="ACHK01_005709"
     CDS             <1..379
                     /locus_tag="ACHK01_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8100397.1"
                     /translation="MISLSPPTICNSAADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGAT
                     EIAHQLSIARSTVYKILEDERAS"
     gene            562..1422
                     /locus_tag="ACHK01_005710"
     CDS             562..1422
                     /locus_tag="ACHK01_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO8100398.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1569..1723
                     /locus_tag="ACHK01_005711"
                     /pseudo
     CDS             1569..1723
                     /locus_tag="ACHK01_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          455 a          384 c          440 g          444 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gccgatatga tccaactgat
       61 aaaggaattt gacgctcagg gcgtggcagt ccggttcatt gatgacggga tcagtaccga
      121 cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg ctgaacgccg
      181 gaggatccta gaacgcacga atgagggccg acaggaagca aagctgaaag gaatcaaatt
      241 tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga agggcactgg
      301 tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata aaattcttga
      361 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctggta aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      781 ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc
      961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1081 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1561 tcagacccct tatggcagag cagggaaagg aattgccggg ctatgtgcaa cgggaatttg
     1621 aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc tgcgagtctt
     1681 gccacgccga gcacctggtc gctttcagct gtaagcgtcg cgg
//
LOCUS       DAZGCS010000541         1119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206T74706
            SAMN43267922-rid23083493.guided.370, whole genome shotgun sequence.
ACCESSION   DAZGCS010000541 DAZGCS010000000
VERSION     DAZGCS010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267922
            Sequence Read Archive: SRR30307996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351312.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351312.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:27:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,618
            Genes (coding)                    :: 5,381
            CDSs (with protein)               :: 5,381
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 96 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 56 of 237
            Pseudo Genes (multiple problems)  :: 44 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267922-rid23083493.guided.370"
                     /serotype="O103:H 2"
                     /isolate="2206T74706"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..942
                     /locus_tag="ACHK01_005712"
     CDS             127..942
                     /locus_tag="ACHK01_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFO8100399.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          298 a          231 c          258 g          332 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc tctgatgtta cattgcacaa
       61 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      121 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
      181 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      241 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      301 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      361 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      421 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      481 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      541 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      601 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      661 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
      721 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
      781 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
      841 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      901 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
      961 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcttccct accctcatgg
     1021 ccgcctccca ggaatcgacc caggtgctta tcgtggacat aatcctttac tcataaattg
     1081 gcactgttgc aaagttagcg atgaggcagc cttttgtct
//
